LOCUS       DATIFR010000001        10305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_191713, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000001 DATIFR010000000
VERSION     DATIFR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191713"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..672)
                     /gene="grdA"
                     /locus_tag="VLD60_00005"
     CDS             complement(181..672)
                     /gene="grdA"
                     /locus_tag="VLD60_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(529..531),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HSB43357.1"
                     /translation="MRRKREMLEGKNVIAFGDGQEIKAESLKKCLEAAGAKVVLVRDA
                     CFVUTAALALDSASQEEIKKAADDHGPENLIVVWGVTNEQYLEVGSMTVMEGDPSWSG
                     PLAGEALHLKVYHVLEPEIKNHIPPDVYEAELMLLELAIGDEKIRKTIAMMEGFRAQR
                     VTR"
     gene            complement(647..2155)
                     /gene="grdC"
                     /locus_tag="VLD60_00010"
     CDS             complement(647..2155)
                     /gene="grdC"
                     /locus_tag="VLD60_00010"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HSB43358.1"
                     /translation="MFWRAEVNASHVIFRKCSNILVHLPDFVRHGSKPSREIKKHPEL
                     EGNIRSRLRTYPEAVSYAPNQVFIGNMEPDTLRTLKQPWFQYLLDRDGAFGRSGPFGE
                     IMPQEEFYALLTAATDESSYDLVWLESRFLAETVSPAIAKHRLCNSADLLQKLRHGKT
                     MEEIRKKIDQDRSLPLYHQDVLVGCFQRHHDEDESLQALVLMENLMTKASAFLALRNL
                     LDFANCEASAIDFIISCSEEAAGDRYQRGGGNLARAIGEMAGCRNAISCDVKSFCVGP
                     HYAILHAAALVKAGLFRKVAVVAGGSLAKLGMKFQGHLNKNMPVLEDVIGGVAFLVTE
                     DDGDSPILRLDLLGKYPIGAGGSQQRIQESLILEPLKAHGLKLTDIDRYATELQNPEI
                     TLPIGQDDIPLKNYRLIAALAVQQKDIEKSQMNEFIENRGLVGFAPTQGHIPAGVSYI
                     AHGIREILEGKKKRIMIVAKGSPFLTRMTNLSDGLSFVLEKNPKNAEEERDA"
     gene            complement(2139..2480)
                     /locus_tag="VLD60_00015"
     CDS             complement(2139..2480)
                     /locus_tag="VLD60_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSB43359.1"
                     /translation="MSPVQHLAEVNEGDFEKEVMEAGIPVLVDFFTPPCAPCKALVPV
                     LERTARKYPNVKVVKVNAWNNQSLCARWKIVGVPALLFFKGGQNVLRIDGFDDSTEKR
                     IEDGLNGVLES"
     gene            complement(2509..3837)
                     /locus_tag="VLD60_00020"
     CDS             complement(2509..3837)
                     /locus_tag="VLD60_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2767..2769),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HSB43360.1"
                     /translation="MSDASRNGKYRIVHYVNQFFGGVGGEEKADCEPFTIQGPQGPGR
                     LLEKLFRQGNVDVEIVATVVCGDSYFVDHQEDVLRELIPLIRGYHPDLVVAGPAFNAG
                     RYGIACGGICSSLQPLGIPAITAMFKENPGVDQYRKTALIVETANNVTGMEEAVRKMV
                     RLGIKRLHGEEIEFPQAEGCFEQGIRKNYSHTEPACERAIAMLLRKINGDPFETEYPI
                     PFFDRVDPRPAIADLKGITIALVTSGGIVPTGNPDHIESSSASKYGKYSLEGLETLDA
                     RTHQSAHGGYDNTYANADPNRVLPIDEMRVLEKEMGFKRHPWYYATVGNGTSVDNAQK
                     FAKEIAVELIRDGVQAVILTSTUGTCTRCGATMAKEIERAGIPVAHICSIITISQTVG
                     ANRIVPAVAIPHPLGNPRLPLEEEKELRRNLLKKALAALQTPIEEQTVFS"
     gene            complement(3838..5193)
                     /locus_tag="VLD60_00025"
     CDS             complement(3838..5193)
                     /locus_tag="VLD60_00025"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HSB43361.1"
                     /translation="MSYARPHAGAHPVTHHWRKITVTDLGYSSATRVENGKLLIDKGE
                     LTALLRSDPNIKDVQLAIARPGDSVRIIPVKDVIEPRLSRIPGYPIFPGVFSAWDPDS
                     HGIPSGEIVSLCGMVVTTVGSIVGYQEGLIDMSGPGARYSLFSGKHHLVLDISVKEGT
                     ERHEHEKSVRLAGFKASDYIARSSLDGSLSSGSRETLINPIDPEYHGSEISDGRKRRP
                     RIAYLYMLQSQGLLHDTYYCGVDVKKMLPRRIVPTEVLFGAIVSGNCVSACDKNTTYH
                     HQNNPIILDLLRRHGVDWDFVGCVITNENVTLGDKQRSSTLAAELIVSLNPDGVIVSK
                     EGFGNPDADLMMNCSKIEAHGIKTVLLTDEFAGQDGASQSLVDSHPNADAIVSTGNAN
                     EVIVLPPMEKVIGDDRVITELAGGSSKCRLPDGSVAIELQALIGSTNQLGFENISSRF
                     K"
     gene            complement(5296..6009)
                     /locus_tag="VLD60_00030"
     CDS             complement(5296..6009)
                     /locus_tag="VLD60_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB43362.1"
                     /translation="MLLSVENLHVSYDNIKALHGLSFRIDEGEIVCIIGANGAGKSTT
                     LRAISRLVPVEAGTKMTFMGRNLLNYPADKVVSELGISHVPEGRRLFDNLTVLENLRL
                     ATFARRDGRAIGEDLDRVFSIFPRLSERQNQKAGTLSGGEQQMLAVGRAFMSGRKIML
                     LDEPSMGLAPLLMKSVFESLKEINREGTTILVVEQNARMALQFAGRGYLLENGRVVLE
                     GRSEELLADPRVKKAYLGG"
     gene            complement(6009..6779)
                     /locus_tag="VLD60_00035"
     CDS             complement(6009..6779)
                     /locus_tag="VLD60_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB43363.1"
                     /translation="MPILRVQNMSHDFGGLRAVQNYDLEIEMGQIRGLIGPNGAGKTT
                     IFNLLTGIYTPTEGEILLDGKRINGLPAHQIASMGISRTFQNLLLWRHMTVLEHVKLA
                     TYSRIRYGLFGAFFGTTKRHKEEAEIERKAYTLLEMMGIRHLSDQVVLNLPYGAQRRV
                     EMARALATEPRILLLDEPTAGMNQEELGQIMEIIRRVHDEFGLAILLIEHRLKVVMEL
                     CERIQTLNFGEVIAEGTPDKIQNDPKVIDAYLGKESVI"
     gene            complement(6781..8064)
                     /locus_tag="VLD60_00040"
     CDS             complement(6781..8064)
                     /locus_tag="VLD60_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSB43364.1"
                     /translation="MAIKVRTSRNLWASYSRVPLLGWFCGALIAVTLERFAGTPIALA
                     LGLPKIPVLFGLDIMLKKPILVPNAILYVFFVYGLPMGVTTRSSISLTNRLASRLIRF
                     PIAITVAVHLALLYTTLHLWAGISDYRSLVLKLTLIAVMLTLSLNVINGYMGEFSCSH
                     PGFMALGAYCASVFTVGLFVDDRFFGAVFLPPALGPFLFPIALILGGFLASFGALFVA
                     IPSFRTRGDYLAIISLAFLFIVKSLIENLEFVRGPRGLSGQPDWADLPTVFLWTVLCV
                     WSINNFVRSTIGKALNAVRDNEMAASAMTVDTRRTKIVAFLFAAFWAGVAGGLFAHVL
                     RYVNPSTFGIQNLAEVLAMVYFGGLNSVYGSIVGAVSISLLGEALRPLEIFKWIIIPL
                     LLILVMIFRPTGLIAFKDLDVTSLLRPKQNHGGEV"
     gene            complement(8069..8992)
                     /locus_tag="VLD60_00045"
     CDS             complement(8069..8992)
                     /locus_tag="VLD60_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSB43365.1"
                     /translation="MVIFLQNLVNALQWGSFYALIALGYSMVYSILMLFNFAHGDIFM
                     VGAYIGFGISIGLLALSSSGALPMPNWLILVLTILFSMFLTSFVGMIVERVGYRPLRN
                     APRASAAITGLMIGIILETGNLILMGARRVSFPSLIETATYNVGGVFVTNKKIMIVLV
                     SLLLTLALHQFVRRTKWGMAMRAMAFDFSVVPLMGVPVNTVIALTFAIGSALAAAAGI
                     LFGVAYPVLDPYMGILFGWKAFVAAILGGRGSILGATLAGFLLGFTEIFVAMVFPSTL
                     RDLIAYSIILLILTFRPHGFFGEPYSARLRL"
     gene            complement(9079..10251)
                     /locus_tag="VLD60_00050"
     CDS             complement(9079..10251)
                     /locus_tag="VLD60_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB43366.1"
                     /translation="MRKGSLFGVCVLVVIMAFSLSGWSQAETIKIGINAPLTGDIPKV
                     GEGTKFAAQMWLEDVKAAGGLAAVKKKYTVELVLEDNESKAESAVKVATKMITEDGVL
                     VMVGPQASKQAIPAGGIANNYKTVMISPWSTNPDTTKGRPYVFRACFLDPFQGPVVAN
                     FISKEFKFTKAAVLYDVASDYPKGLAEFFKKAWEDQHGKGSVVAYESFTTKDTDFSSQ
                     LTKIINSGAQVLFTPQYYNEVALIVQQAHQLGWKKPIVGSDSWGSAETVKLCGKDCYG
                     AFFSTHYAAAGATGATKAFIDRYKAKYGYVPDDVAALTWDSMLLVQQAIQNTGGLTGD
                     LKKDRENVRAALSQIKKFKGITGEMTFAGGGDPVKCAVIVKISDAGEFTFYKSVCP"
BASE COUNT         2205 a         3017 c         2845 g         2238 t
ORIGIN      
        1 tcggagatgt ccatccctgc gcaatcgtag cccaatttag aaaaatatat acgtagtttc
       61 ccgctccaca gcccataagg aataacttcg ttccgagaga aaagaggggg cagaatctaa
      121 aggatcctat ccgacggtcg ccctccaatg accatcggtc gcaaccgaaa cgaaaccgac
      181 tcatctggtt acccgttgag cgcggaaccc ctccatcatg gcaatcgttt tcctgatctt
      241 ttcgtcacca atcgcaagtt ccagaagcat cagctctgcc tcgtacacat ccggaggaat
      301 gtgatttttg atttccggtt ccaagacatg ataaaccttc aggtgcaatg cctctcccgc
      361 caaggggccg gaccagctgg ggtcgccctc catgaccgtc atggagccga cttcgagata
      421 ttgttcgttg gtgacccccc acacaacgat aaggttctcc ggcccatgat catctgcagc
      481 cttcttgatc tcttcttgac tcgccgagtc aagcgccagg gcggctgttc agacgaaaca
      541 tgcatcccga accagtacaa ccttcgcccc ggccgcctcc aggcactttt tcagtgactc
      601 tgccttgatc tcctggccgt ccccgaatgc gatcacattt ttcccctcaa gcatctctct
      661 cttcctccgc atttttcgga ttcttttcca agacgaagga cagaccgtcc gacaggttcg
      721 tcatacgtgt caaaaacgga cttcccttgg ctacgatcat gatacgcttt tttttcccct
      781 ccaggatttc ccggattcca tgcgcgatgt aagagacccc ggcagggata tgtccctgcg
      841 ttggggcaaa gccgaccaac cccctattct cgataaattc gttcatctgg gacttttcga
      901 tatccttctg ctgaacggcc aacgccgcga tgagccgata gttcttcagg gggatatcat
      961 cctggccgat cggcagcgtg atctccggat tctgcagttc ggtcgcatag cggtcgatgt
     1021 ccgtcaattt caatccgtgg gctttcagcg gctccagaat caggctctcc tgaatgcgct
     1081 gctgcgaccc tcccgccccg atcgggtatt tccccaacag atcaagccga aggatgggtg
     1141 aatcgccgtc gtcttcggtt accaggaatg ccactccccc gatcacgtct tccagaaccg
     1201 gcatgttttt attgagatgg ccctggaatt tcattcccag cttggcgagc gaccctccgg
     1261 ccacgacggc taccttccgg aacaggcccg ccttgaccag agcggccgca tggagaatgg
     1321 cgtaatgggg gccgacgcag aacgatttta cgtcacagga aattgcgttc cggcacccgg
     1381 ccatttcgcc gatggcgcgg gcgagattgc cgccgccgcg ctggtaccgg tcgccggccg
     1441 cctcttccga acagctgatg atgaaatcga tcgccgacgc ttcgcagttc gcgaaatcga
     1501 gcagattccg gagcgccaga aatgccgaag ccttggtcat cagattttcc atcagcacga
     1561 gcgcctgcag cgactcgtct tcgtcatgat gccgctgaaa acatccgacc aggacatcct
     1621 gatggtaaag aggcaacgat cggtcctggt ctattttctt ccggatctcc tccatcgtct
     1681 ttccgtgacg cagcttttga agcagatcgg cggaattaca caggcgatgc tttgcgatcg
     1741 ccggcgacac ggtttcggcc agaaaccgac tttccagcca gaccaggtcg taagaggatt
     1801 catccgtcgc cgccgtcaag agagcgtaga actcctcctg aggcatgatc tccccgaagg
     1861 ggccggagcg gccgaacgcg ccgtcacgat ccaggagata ttggaaccac ggctgtttca
     1921 acgttcggag cgtgtccggc tccatgttcc cgatgaagac ctgattggga gcgtaactca
     1981 cggcttccgg gtaggtcctc aatcggcttc gtatgtttcc ctccagttcc ggatgctttt
     2041 taatttccct ggatggtttg gagccgtgac ggacgaaatc aggaagatgt acgaggatat
     2101 tggaacattt ccggaagatg acatggcttg cattcacttc agctctccag aacaccgttc
     2161 aagccgtctt cgatccgctt ttcggtcgag tcgtcgaatc cgtcgatccg aagaacgttt
     2221 tgtccgccct tgaaaaaaag aagagccgga acacccacga tcttccacct tgcgcaaagg
     2281 ctttggttgt tccaggcatt gaccttgacg actttgacgt tcggatattt tctggctgtt
     2341 ctttccagga cgggaacaag agctttacag ggggcacacg gaggagtaaa aaaatccacg
     2401 agaacgggga tccctgcctc cattacctcc ttctcgaaat ccccctcgtt gacttcggcg
     2461 agatgctgta ccggactcat ctgggggaac cccggccttt cgtcggtgtc atgaaaaaac
     2521 ggtctgctct tcgatgggcg tctgaagcgc ggcaagcgct ttcttcaaca ggttccgccg
     2581 cagttctttc tcttcctcca gcggcagcct ggggtttccc agcggatggg gaatggcgac
     2641 ggccggaacg atccggttgg cgccgaccgt ctgggatatg gtgatgatgg aacagatgtg
     2701 cgccaccggg atcccggcgc gctcgatctc ttttgccatc gttgccccgc aacgagtgca
     2761 ggtccctcaa gtggacgtga ggatgacggc ctggaccccg tcccggatca actcgaccgc
     2821 gatctccttc gcgaatttct gggcgttgtc caccgaggtt ccgtttccca ccgtggcgta
     2881 ataccaagga tgccgcttga atcccatctc cttttccaga accctcatct catcgatggg
     2941 aagcacccgg ttggggtcag cgttggcata ggtgttgtcg tatccgccgt gagcggactg
     3001 atgggttcgg gcatccagtg tctccagccc ttcaagggaa tatttcccgt atttggacgc
     3061 actcgagctt tcgatatggt ccggattccc cgtgggaacg atgccgccgg aggtgaccag
     3121 ggcgatggtg atccctttca ggtcggcaat ggccggccgc ggatcgacac gatcgaaaaa
     3181 ggggatcggg tattcggttt cgaacgggtc cccgttgatt ttccgaagga gcatggcaat
     3241 agcgcgttca catgccggct cggtatggga ataatttttc ctgatgccct gttcgaagca
     3301 cccctcggcc tgggggaact cgatctcctc gccgtgcagc cttttaatgc cgagccggac
     3361 cattttccgg accgcctcct ccatcccggt tacgttgttg gcggtttcga cgatcaaagc
     3421 cgtctttctg tattgatcca caccggggtt ctctttgaac atggcggtga tggcagggat
     3481 gccaagcggt tgcagcgacg agcagatgcc gccgcaggct atgccgtatc gtccagcgtt
     3541 gaacgccggc ccggcaacga ccaggtccgg atggtatccc cggatcaatg ggatcagttc
     3601 acgaaggacg tcttcctggt ggtcgacgaa atagctgtcg ccacagacga ccgtggcgac
     3661 gatttcgaca tccacattcc cttggcgaaa cagtttctcc agaaggcggc cggggccttg
     3721 cgggccttgt atcgtgaagg gttcgcagtc cgccttctct tccccgccca cacccccgaa
     3781 gaactggttc acataatgga cgatccggta tttcccgttc cgggaggcgt cgctcatcta
     3841 cttgaacctc gaggatatat tttcaaatcc cagctggttg gtggacccga tcagcgcctg
     3901 cagctcgatg gcgacgctcc cgtccggaag acggcatttg ctggagccgc cggccagctc
     3961 cgtgatgacg cggtcgtcgc cgatgacctt ttccatcggt gggagaacga tcacttcgtt
     4021 cgcgtttcct gtggaaacga tggcgtcggc gttcgggtgg gagtccacca aggattgcga
     4081 ggcgccgtcc tgtcccgcga attcgtcggt gagcagaacc gttttgatgc catgcgcctc
     4141 gatcttggag cagttcatca tgagatcggc gtcgggattg ccgaagccct ccttggaaac
     4201 gatgacgccg tcgggattca gggaaacgat cagctcggcc gccagcgtgg acgaccgctg
     4261 tttgtcgccc aaggtgacat tttcgttggt gatgacgcag ccgacgaagt cccagtccac
     4321 cccatgcctg cgtaggagat cgaggatgat ggggttgttc tggtgatgat atgtcgtgtt
     4381 cttgtcgcac gccgagacac aatttccgga gacgatggcg ccgaatagca cttccgtagg
     4441 aacgattcgc cttggcagca tcttctttac atccactccg caatagtacg tgtcatgcag
     4501 aaggccctgg gattggagca tgtacaggta ggcgattctg ggcctccttt tccgtccgtc
     4561 ggatatttcc gaaccgtggt actccggatc gattggattg atcagggttt cgcggctgcc
     4621 ggaggaaagc gacccgtcca aggaggatcg ggcgatataa tcggatgcct tgaaacctgc
     4681 caaccggacc gatttttcgt gttcgtgccg ttccgtcccc tcttttacgg aaatatccag
     4741 caccagatgg tgtttgccgg aaaagagcga gtaccgggcg cccgggccgg acatgtcgat
     4801 gagcccttct tggtagccga cgatcgatcc cacggtggtc acaaccatcc cgcacaacga
     4861 cacgatctcc ccggaaggga tcccgtggga atccggatcc catgcggaaa acaccccggg
     4921 aaagatcgga taccccggga tcctgctcag gcgaggttcg atgacatcct tgacgggaat
     4981 gatccgaacc gaatccccgg gtcgggcgat cgccaattga acatctttga tgttgggatc
     5041 ggatcgtaac agggcggtca actcaccttt gtcgatgagc aacttcccgt tttccacccg
     5101 ggtggctgag gagtatccca gatccgtgac ggtgattttt ctccaatgat gcgttacagg
     5161 atgcgcaccc gcatgaggtc tcgcgtagga cataattccc tcccacagag cgggccgata
     5221 tctcaaagat tcggtttacg tagtatacca tcgccaccgc acgggcagga tgaccgatgg
     5281 cgtttttctc ggcgatcacc ccccgagata cgctttcttg acccgggggt cggcgagaag
     5341 ttcctcggac ctgccctcga gcacgactct gccgttctcc agcaaatacc ctcgacctgc
     5401 gaattggagc gccatgcggg cattctgctc gacaaccagg atggtggttc cttcccgatt
     5461 gatttccttc agcgactcga acacgctctt catcaacaag ggagccaacc ccatggaggg
     5521 ctcatccagg agcatgattt tcctgccgct catgaatgcc cgcccgacgg ccagcatctg
     5581 ctgctctccg ccgctcagcg ttccggcctt ctgattctgg cgctcggaaa gccgagggaa
     5641 gatcgagaat acccggtcca ggtcttcccc gatcgctctg ccgtccctcc ttgcaaaggt
     5701 ggctagccgg aggttctcca ggaccgtcag gttgtcaaac agacgtcttc cttcggggac
     5761 gtgggagatt ccgagctcgc tgaccacctt gtcggcagga taattgagca agttccggcc
     5821 catgaaggtc attttcgtcc ccgcttcgac agggacgagg cgggaaatcg ctcgcaaggt
     5881 agtgctcttc cccgctccgt tggcgccgat gatgcacacg atctccccct catcgatccg
     5941 gaagcttaac ccatgaaggg ccttgatatt gtcgtaggaa acatgcagat tttccacgga
     6001 cagcaacatc agatcacgct ctccttgccc aaataggcat caatcacctt gggatcgttt
     6061 tgaatcttat caggcgtgcc ctcggcgatg acctcaccga agttcagggt ctgaatcctc
     6121 tcgcagagct ccatgaccac cttcagcctg tgctcgatga ggaggatcgc aagcccgaac
     6181 tcatcatgga cccgacggat gatctccatg atctgaccca gctcttcctg gttcatcccc
     6241 gccgtgggct catccagcag caatatcctg ggttccgttg ccagtgcccg ggccatctcc
     6301 acccttctct gggcaccgta gggcaggttg aggaccacct ggtccgaaag gtggcggatg
     6361 cccatcattt ccaggagcgt atatgctttc ctttcgatct ccgcctcttc tttgtggcgc
     6421 ttcgttgtac cgaagaaggc cccgaacaat ccgtaccgga ttcgcgagta ggtggccaac
     6481 ttaacgtgct cgaggacggt catgtgtctc cacaggagaa ggttctgaaa ggtcctgctg
     6541 atgcccatgg aggcgatctg gtgagccgga aggccgttga ttctcttgcc atccagcaga
     6601 atttcccctt ccgtgggcgt gtagatgccg gtaaggaggt tgaagatggt ggtctttcct
     6661 gcaccgttcg gcccgatcaa cccccggatc tgacccattt cgatctcaag gtcgtagttt
     6721 tgtacggccc gcaatccacc gaagtcgtga gacatgtttt gaacccgaag gatcggcatg
     6781 tcacacctct ccgccgtgat tctgtttcgg ccggagaagg ctcgtgacgt cgaggtcttt
     6841 aaatgcgatc aggcccgtcg ggcggaaaat catcacgagg atgagcagga gcggaatgat
     6901 gatccacttg aaaatctcca gaggccggag ggcttcaccg agtagactga tgcttacggc
     6961 gccgacaatg gatccgtaaa cggagttcag tcccccgaag tacaccatcg ccaggacttc
     7021 ggcaaggttt tgaatgccga aggttgacgg gtttacgtat ctcagcacgt gggcaaaaag
     7081 ccctcccgca acgccggccc agaaggctgc gaaaagaaac gccacgatct tggtgcgtct
     7141 cgtgtcgacc gtcatggcgc tggccgccat ttcgttgtcc cgaaccgcgt tgagggcctt
     7201 tccgatcgta gagcgcacaa aattgttgat gctccatacg cagaggacgg tccagagaaa
     7261 caccgtgggc aggtcggccc aatccggctg cccgctcaag ccccgcgggc cccgaacgaa
     7321 ttcgaggttt tcgatcagac tctttacgat gaataggaac gccagggaaa taatggcgag
     7381 gtaatcccct ctcgtccgaa aggagggaat cgcgacaaac aaggccccga aggatgccag
     7441 aaatcctccc aggatcaggg caatggggaa caggaacggt cccagggccg gagggaggaa
     7501 tacggcccca aagaatcgat catcgacaaa cagccccacg gtgaacaccg atgcgcagta
     7561 tgcgcccagg gccatgaacc cggggtggga acaagagaac tcgcccatat agccgttgat
     7621 cacattcaag ctcaaggtga gcatgacggc gatcaaagtg agcttgagaa cgagggagcg
     7681 gtagtcgctg attcccgccc acagatgcaa ggtggtgtaa agcaatgcaa gatggacggc
     7741 caccgtgatc gcaatcggaa accggataag ccggctggca agccggtttg tcagagagat
     7801 gctggaacgt gtcgtgactc ccattggcaa tccgtataca aaaaacacgt aaaggatcgc
     7861 gttggggacc aggatgggct ttttcaacat gatgtccaaa ccgaacagga ccggtatttt
     7921 gggtaatccc aaggccaatg cgataggggt gcccgcgaac cgttccagag tcactgcgat
     7981 caaggcgccg caaaaccagc ccagcagcgg gacccgggaa taggaggccc agagattccg
     8041 ggatgtccgt acctttatgg ccaaaacttc agagcctcaa ccgtgcactg taaggttcac
     8101 cgaaaaaccc gtgaggacga aacgtcagga tcagcagaat gatggagtag gcgatcaggt
     8161 cgcgcaacgt cgaggggaag accatggcca cgaagatttc ggtgaacccc agcaagaagc
     8221 cggcgagagt ggctcccagg atagagcctc ttccccccag gattgccgcc acgaacgcct
     8281 tccaaccgaa cagaataccc atataaggat ccaatacggg gtaggcaacg ccgaagagga
     8341 tcccagcggc ggcggccagg gccgaaccaa tcgcgaacgt gagagcgatc acggtgttga
     8401 ccggtacccc catcaagggt acgacggaaa aatcaaatgc catcgcccgc atggccattc
     8461 cccacttcgt cctgcggaca aactggtgca gggcaagggt aaggagcaac gacacgagaa
     8521 cgatcatgat tttcttgttg gttacgaata cgccacccac gttataggtt gcggtctcga
     8581 tcagggacgg gaaactgacc cgacgtgctc ccatcaggat caggttgccg gtttccaaga
     8641 tgatgccgat catcaagccc gtgatggcgg ccgaggcccg gggggcgttc cgcaacggcc
     8701 gataccctac ccgctccaca atcatgccca cgaaagaggt caggaacatg gaaaagagga
     8761 tggtcaacac caggatcaac cagttgggca ttggaagggc gcccgaggag gaaagcgcaa
     8821 gcaggccgat ggaaataccg aagccgatgt acgccccgac catgaagata tcgccatgcg
     8881 caaagttgaa gagcatgagg atgctgtaca ccatggagta tcccagcgcg atcagagcgt
     8941 agaagctccc ccactggaga gcgttgacaa ggttttgcag gaaaatcacc atcgcttcgg
     9001 tccggtcctt tcaacaaagg cgggctcaga gcctccgctc tggcccgcct ttccaaaaac
     9061 gaatcccggc ggcgcccctt acgggcagac cgacttatag aacgtgaact cgcccgcgtc
     9121 gctgatcttg acgatgaccg cgcacttgac cgggtcgcct ccgccggcga aggtcatctc
     9181 tcccgtgatg cctttgaatt tcttgatctg ggacaacgcg gcgcggacat tttcccggtc
     9241 ctttttcaga tctcccgtga gcccgccggt gttctggatc gcctgttgaa cgagcaacat
     9301 ggagtcccaa gtgagcgcag ccacatcgtc gggcacgtaa ccgtattttg ctttatacct
     9361 gtcgatgaac gccttggtcg ccccggtcgc acctgcggca gcgtagtggg tactgaaaaa
     9421 agccccataa caatctttgc cgcatagttt taccgtttcc gccgaccccc agctgtcgct
     9481 ccccacgatg ggttttttcc accccaactg gtgggcctgc tgcacgatca aggcaacctc
     9541 gttatagtac tggggggtga acagcacctg ggcgccggaa ttgatgatct tcgtcaactg
     9601 ggagctgaaa tcggtatcct tggtggtgaa actttcgtac gccacgacgg aacctttgcc
     9661 atgctggtct tcccaggctt tcttgaagaa ctcggcaagt cccttggggt agtcactcgc
     9721 cacgtcgtac aggactgcag ccttggtgaa tttgaactcc tttgaaatga aattggccac
     9781 caccggaccc tggaagggat cgaggaagca ggcacggaaa acgtagggtc gtcccttggt
     9841 ggtgtcgggg tttgtggacc atgggctgat catcaccgtc ttgtagttat tcgctatccc
     9901 gccagcgggt atcgcttgtt tggaggcctg aggtccaacc atgacgagta cgccgtcctc
     9961 ggtaatcatc ttggttgcga ccttcaccgc ggattccgct ttggactcat tgtcctcgag
    10021 aaccagctct accgtgtact tctttttcac cgccgcaaga cccccggcgg ctttcacatc
    10081 ttcgagccac atctgagccg cgaacttcgt gccctcgccc accttgggga tatcgccggt
    10141 gagcggggcg tttatgccga tcttgatcgt ctctgcctga gaccaaccag aaagtgagaa
    10201 cgccatgata accacgagga cacaaacacc gaacagtgat ccttttcgca tcgccaattc
    10261 cctcctttgg tttccagtga ggttccgtta attcacgaca caacg
//
LOCUS       DATIFR010000002         1042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1533010, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000002 DATIFR010000000
VERSION     DATIFR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533010"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..925)
                     /locus_tag="VLD60_00055"
     CDS             complement(join(168..586,586..925))
                     /locus_tag="VLD60_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSB43367.1"
                     /translation="MSADLFWFDDEQWSKVQPHLPTNQRGPERHDDRRILSGIMHVQK
                     VGCRWKDCPSEYGPHKTIYNRFARWSERGIWQNIFAAVAACSNTPEQVALDSSHVKIH
                     RCASGGKGGLRPQAIGITKGGRNSKVHAVVDNLCRPWVLVLTPGNTADCVMAEACVSL
                     IPGVTQLLADKGYDTDAFRAFLKDNDIKAVIPAKSNRKKRIRHDKQAYKGRNVVERCF
                     CRLKDFRRIATRYDKLARNFFSALCFVATLAYWL"
BASE COUNT          206 a          308 c          302 g          226 t
ORIGIN      
        1 gattcggcgc gctccctccc cttgaaaagg ggagggtcgg ggtggggatc gttccctttg
       61 cacgcgcgct tgtgatcccc acccgccggg cgatcgcgca tcgcggacgc acgtgaatgc
      121 gcttacgggc cgggccgcag caagcctagg gtccggactc aattaggtca cagccagtac
      181 gccagggtag cgacgaagca cagggcagag aagaaatttc tggcgagctt gtcgtagcgc
      241 gtagcgatgc gtctgaagtc cttgagacga cagaagcagc gctcgacgac gttgcggcct
      301 ttgtaagctt gtttgtcatg acggatgcgc ttcttgcgat ttgatttggc gggaatgacc
      361 gctttgatgt cgttgtcctt gaggaaggcg cggaacgcat ccgtatcata gcctttgtcc
      421 gccagaagtt gcgtgacgcc ggggattagg ctgacgcagg cttcggccat cacgcagtcg
      481 gcggtgttgc ccggcgtgag gaccagaacc cagggacgac acaagttgtc gacaaccgca
      541 tgaaccttgc tgtttcgacc gcctttggtg atccctatgg cctgcgcctg agcccccctt
      601 ttccgccgct ggcgcagcgg tggattttca catggctgct gtccagcgcc acttgttcgg
      661 gcgtgttcga acatgccgca acggcggcga atatattctg ccaaattccc cgctcgctcc
      721 aacgcgcgaa gcgattgtag atcgttttgt gcgggccata ctcgctcggg cagtccttcc
      781 agcgacaccc caccttctgc acatgcatga tcccgctcaa aatccgacga tcatcatgcc
      841 gctcgggacc acgttggttg gtcggcaaat gcggctgcac tttcgaccac tgctcatcgt
      901 caaaccaaaa caaatctgcg ctcatgggtc tggccctccg aatcggttga ccaaaccttt
      961 gaatccgaag acgattaatt aggtcctaac cctaggagat gtgggatttg caccaaagct
     1021 gaatctccga ccaacgcaca gt
//
LOCUS       DATIFR010000003         7798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_383668, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000003 DATIFR010000000
VERSION     DATIFR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383668"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1981
                     /locus_tag="VLD60_00060"
     CDS             <1..1981
                     /locus_tag="VLD60_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB43368.1"
                     /translation="NHVSILVKVGAIFIVLSLTFGTFLLTTSNLTRHLIGTSKAIEQA
                     GSERMRIYKLASLVQQLPGDSSPKIGELIRAEIAQWERVLEGLRYGTSDHGPIGEIAT
                     TLPGQLQELQDKWSMQLRPALELAMGAAGENLVVQQQAYVVHADEFVAGLDEMVRSLE
                     RESAARLQSLYLLQIWFLVASVVLMSAALFVLHRIIREPLKRLTEGAERLATGELHTQ
                     IQVHAQDELGQLARTFERMAGTIRQKIEDMRALHATGQEIVILGPGGLDEVLRRIVDR
                     AAESLDADLAVVMARHLTMECWLVEAASGTAFDRIRKQILVVEETPFSNQAFETRRPV
                     VVHNLSDYPDRPIRFRDEFGAQSYVAVPLLGPHGCLGVLVLLSIARVRTFTEWDIRLA
                     EQFASYAAVTMENARLFDEVESESHNLRVKLQAVERNVAELTHEVKGPAGRVAEFASW
                     IEQDYGPRLDSKGIQYLDWIKKEGKDLAHLAERTLDLARINHAPSPLESVDVDAVVRE
                     VLALLRTDCAEKHIRVTIAPGLPRLGCRRIHVKQIFENLIGNAIKYMGDQPDPHLEIG
                     TIESESGPLLFVRDNGIGIDPSMADRIFLPFQRLAPGNIPGSGIGLSIVKTVAEQYEG
                     AVSVDSSPGVGSTFYVRLPVLPMKPSFAADRVQTS"
     gene            2000..3463
                     /locus_tag="VLD60_00065"
     CDS             2000..3463
                     /locus_tag="VLD60_00065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB43369.1"
                     /translation="MTKTTSPAVQETGMPSIPNANAKVFTILLVEDNGGDVEMFLRTV
                     EEELPRDEDEQVELVIAARAEGGLQILDERRIDLVITDIRLPGMSGIELLQRIQARDR
                     RIPVIMISWVDTVDTAVEAMRCGAFDYVTKPFEKLDLAARIHRAMRISEILFRYEPPQ
                     PHDGPPFKELVGVSPAIQNVMAMIEAAARVQSTTLIIGETGTGKELIARAIHERSAER
                     DGPFQVVDCTTFSEGTVESELFGHVRGAFTGAVGDKLGLIEGGFGGTLFLDEIGDLPL
                     PLQAKLLRVLEEGEMRAVGSVQTKKIAARFIAATNQELTEKVKRNEFRKDLFFRLNVM
                     AIKVPPLRERTQDIPLLARHFITRYTKEFGKYVADLHPSAVTELIAYPWPGNVRELRN
                     VIERAVMLAKGDRLSHDDVIGLLQFTDQARRSQTEDYLSLPYAKAKEKVLEDFSRRYI
                     KSKLDHHGGNVTHAAQEAGLPRSYFHEIMRRYVKEDT"
     gene            3925..7371
                     /locus_tag="VLD60_00070"
     CDS             3925..7371
                     /locus_tag="VLD60_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB43370.1"
                     /translation="MQLSHVSRRQFLKITAGTVAAVAVADKVLALTALQPVIEVGNPL
                     GDYPDRSWERVYHDQYRYDSSFTWCCSPNDTHGCRIRAFVRNGVVMRVEQNYDHQTYE
                     DLYGNRGTFAHNPRMCLKGYTFHRRVYGPYRLKGPLMRKGWKEWMDAGAPELTPDVKR
                     KYKFEARFLDDMMRVSWDTAATYAAKGCIIIATRYSGEAGARRLREQGYAPEMIEMMK
                     GAGVRCFKHRAGMPVLGIIGKMMNTRFNGGVLPLLDSWIRKVDPERAQGGKYYSNYTW
                     HGDQDPSHPWWNGTQNCDIDLSDMRFSKLNTSWGKNFVENKMPEAHWKLESIERGARI
                     VVVTPEYNPTAYRADYWIPVRPETDGALFLSACKIIVDENMQDIDYIKQFTDMPLLVR
                     TDTLQYLDPRDVIPDFKFPDFSKSYSGRIQALKPEHIERLGGFMVWDQVKKQAVFLHR
                     EQVGFHFVQSGIDPALTGTYRVKLLNGREVDVSPIYQMYLVHFQDYDLDTSHQITRAP
                     KDLIVRWARDSGTIKPAAMHNGEGVCHYFHMTEMGRAAALVLTLTGNIGKFGTGCHTW
                     SGNYKAGIWNATPWSGAGLAVHTGEDPFNQTLEATAHGKEIKTKSYYYGEEVGYWNHG
                     DTALIVNTPKYGRKVFTGKTHMPSPSKVRWVTNVNILNNAKHHYDMVRNVDPNIEMIV
                     TQDIEMTSDVNHADIAFACNSWMEFTYPEMTATVSNPWIQIWKGGIRPLYDTRNDADT
                     FAAVAVKLTEMTGDVRMRQVFHFVYQNRVDVYAQRVLDASSTCYGYSADIMLKSEKGW
                     MVMTRTYPRHPLWEETNESKPQWTRSGRIETYRIEPEAIEYGENFVVHREGPEATPYL
                     PNAIFTSNPYVRPDDYGIPITAQHHDDKHIRNIKLPWQEIKRNANPLWEKGYQFYCVT
                     PKTRHRVHSQWSVNDWVQIYESNFGDPYRMDKRTPGVGEHQLHINPQAAKDRGINDGD
                     YCYVDGNPVDRPYRGWKPSDPFYKVARLMIRCKYNPAFPYHVTMAKHAPYVSTAKSVK
                     GHETRPDGRAIAVDTGYQSNFRYGAQQSFTRSWLMPMHQTDSLPGKHAIAWKFKWGYA
                     IDHHAVNTTPKECLIRITKAEDGGIGARGPWEPVRTGFTPGQENEFMIKWLKGEHIKI
                     KV"
     gene            7470..>7798
                     /locus_tag="VLD60_00075"
     CDS             7470..>7798
                     /locus_tag="VLD60_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit beta"
                     /protein_id="HSB43371.1"
                     /translation="MPEVYNWQLGRKMLYPYEERHPKWQFAFVFNINRCLACQTCSMA
                     DKSTWLFSKGQEYMWWNNVETKPYGGYPQFYDVKITQLIEQVNPGGQVWNVRVGRKHH
                     APYGVFEG"
BASE COUNT         1813 a         2169 c         2276 g         1540 t
ORIGIN      
        1 caaccatgtg tcgatcctcg tcaaagtcgg agcaattttt atcgttctct ccttgacctt
       61 cggaaccttt ctcctcacca cctccaatct cacaaggcat ctgatcggga caagcaaagc
      121 catcgaacag gcggggtctg aacgcatgcg catctataag ttggcctctc tggttcaaca
      181 actgcccggc gactcctccc cgaagatcgg cgagttaatt cgagcggaga tagcccagtg
      241 ggaacgggtg ctggaagggc tgcgctatgg aacgtcggac cacggaccga tcggcgaaat
      301 cgccacgacg ctcccaggac agcttcagga acttcaagac aagtggtcga tgcaactcag
      361 gccggccttg gaactggcaa tgggcgccgc cggcgaaaat cttgtcgtcc aacagcaggc
      421 ctacgtggtg catgcggatg aattcgtcgc cgggttggac gagatggtgc gatcgctgga
      481 gcgggaatcg gctgcccggt tgcaatcgct ctatctcctg caaatctggt ttctcgtggc
      541 ttccgtcgtg ctgatgtcgg cggccctgtt cgtcctccat cgtatcatcc gcgaaccgct
      601 caaacggctc acggagggag ccgagcgtct cgccaccggc gaacttcaca cacagataca
      661 agtccacgcg caggacgaac tgggacaact ggcgcgcacg ttcgagcgga tggcgggcac
      721 cattcggcaa aagatcgaag acatgagagc actgcatgca acaggacagg aaatcgtcat
      781 actcggaccc ggaggtctcg acgaggtatt gcggcggatc gtggaccgcg ccgccgaatc
      841 cctggatgca gacctggcag tggtgatggc tcgccacttg acgatggagt gctggttggt
      901 cgaagcggcg tcggggacag cgttcgatcg gattcgaaaa caaatcctcg tcgttgaaga
      961 aacacccttc tcgaatcagg cgttcgagac cagacgtcct gtggtggtcc ataatctctc
     1021 agactatccg gacagaccca tacgattccg ggatgagttc ggagcacaga gttatgtggc
     1081 cgtccctctc cttggtcctc acgggtgtct cggtgtcctc gtgctcctga gcatagcgag
     1141 ggtgaggaca tttacagaat gggacatccg tctcgctgag cagtttgcgt cttatgcagc
     1201 agttaccatg gaaaacgccc gcttgttcga cgaggtcgaa tccgagtcgc acaatttacg
     1261 ggtgaagctt caggccgtcg aacgcaacgt tgccgaactg acccatgagg tcaaaggacc
     1321 ggccggccgg gtcgctgagt tcgcttcctg gatcgaacag gactacggcc cgcgcctgga
     1381 cagcaaaggg atccaatatc tcgattggat caagaaggaa ggcaaggatc tggcacattt
     1441 ggcggaacga acactcgatc tcgcgcggat caaccatgca ccctcccccc tggaaagcgt
     1501 cgatgtcgac gcggtcgtcc gcgaagtgtt agctcttctg agaacggact gcgcagaaaa
     1561 gcacatccgg gtaaccattg cccccgggct gccgcgactc gggtgccgac gtattcatgt
     1621 caaacagatc ttcgagaacc tcatcgggaa cgccatcaag tacatgggtg accagccgga
     1681 cccccatctg gaaatcggca cgatagagag cgaaagcggt ccgctcttgt tcgttcgaga
     1741 caacggcatc ggcatcgatc cgtcgatggc ggaccgtatc tttctccctt ttcagcggct
     1801 cgcacctgga aacattcctg gctccgggat cgggctttcg atcgtgaaaa ccgtggccga
     1861 gcagtatgaa ggggccgtga gcgttgactc cagtccaggt gtgggaagca ccttctacgt
     1921 tcgacttcct gtcttaccca tgaagccatc cttcgcagca gatcgagtgc aaacgtccta
     1981 gcccacttga ggtgttgtca tgaccaagac cacgtcacca gcggttcaag aaaccggcat
     2041 gccctccata cctaacgcga acgccaaggt gtttaccatc ttgctcgtag aggataacgg
     2101 cggagatgtc gaaatgttcc tccgcaccgt ggaggaggaa ctgcctcgcg atgaggacga
     2161 gcaggtcgaa ttggtcatcg ccgcgcgcgc cgaaggcggg ttacaaatcc ttgatgaacg
     2221 acgaatcgat ttagtgatca ccgatattcg tcttcccgga atgagtggga tcgaactgct
     2281 gcagcgcatt caagcgcgag accgtaggat tcccgtgatc atgatcagct gggtcgatac
     2341 ggtagatact gctgttgagg ctatgcggtg cggagccttc gattatgtca cgaaaccgtt
     2401 tgaaaagctg gatctcgccg cgcgcattca tcgcgcgatg cggatctccg agattctatt
     2461 ccgttacgaa ccgcctcagc cccatgacgg tcctcctttc aaagaattgg tcggcgtcag
     2521 cccggccatt caaaacgtca tggccatgat cgaagccgcc gcccgggtcc agtccacgac
     2581 gctgattatc ggtgagaccg gtacgggaaa agagctcatc gcgagagcaa tccacgaacg
     2641 aagcgcggaa cgagacggcc ccttccaggt cgtggattgc acgacgttct cagagggcac
     2701 ggtcgaaagc gagctcttcg gccacgtgcg aggcgcattc acgggggccg tcggggacaa
     2761 gctcggactc attgaaggcg gattcggcgg cacactgttc ctcgatgaga tcggcgactt
     2821 gcctctaccg cttcaggcaa agctcctgcg cgtgctggag gaaggggaaa tgcgggcagt
     2881 cggtagcgtc cagacgaaga aaattgctgc gcggttcatc gcggctacga atcaggaact
     2941 caccgaaaag gtcaagcgaa acgaatttcg caaggatttg ttcttcaggc tcaatgtcat
     3001 ggcaatcaaa gtcccgccct tgcgtgaacg gacacaggac attcccctcc tcgctcgcca
     3061 ttttataaca cggtacacga aagaattcgg gaagtacgtc gctgatttgc atccgtcggc
     3121 ggtgacggaa ctgatcgcct atccttggcc cggaaacgtt cgtgagctgc gcaacgtcat
     3181 cgagcgggcc gtgatgctgg cgaagggcga ccgtctgagt cacgacgatg tcatcggact
     3241 gctgcagttc acggatcagg ctagacgcag ccaaacggag gactacctct ccctgcccta
     3301 cgccaaagcg aaggaaaagg tgttagaaga tttcagccgt cgatatatca agagcaagct
     3361 cgaccatcat ggaggaaacg tcacgcatgc cgcgcaagag gccggccttc cgcgatccta
     3421 ttttcacgaa atcatgcgac gatatgtaaa agaagatacc tgacaatcag ctgtcatgat
     3481 ttcctgacaa ctcatccttt aattcgcgcg ttaggccccg ccatttcaga gctttcgcgc
     3541 tcgatgtaca actgtcatcg attcctgaca taagaatcat ctatcaatcc gcgcccttgg
     3601 cttctttaga tgctaagagc gtcaggcaat actgacactg cctctatttg attcggttga
     3661 tgctgccagc cgatcccatt tagctccccg ccaactgatt gaattatcga cgaatcgcgc
     3721 acacgaatgc catgcgacat ttttttgtca aatggcatgc cgcttgtaaa gcatacgtgc
     3781 tgttaaccgc tatgtcctga gactgctatg tcgcgttgta tcaagcgtca gaaagaaagg
     3841 ggggtgatct gaaaggctct tggcctttca tgcagaagct gaggtaacag cagcaagatt
     3901 atcacgaagt aggaggtgtt cgagatgcag ctatcacatg tatcacgtcg acaattcctc
     3961 aagattacag caggtacggt cgccgccgtg gcggtggccg ataaagtcct ggcgttgacc
     4021 gcgctgcagc cggtcatcga agtcgggaac ccgctgggtg actacccgga tcggtcctgg
     4081 gagcgggtct atcacgacca gtaccggtac gattcctcat ttacatggtg ctgttcgccg
     4141 aatgatacgc acggttgtcg gatccgggcc ttcgtccgga acggcgtcgt gatgcgggtc
     4201 gaacagaact acgaccacca aacctatgaa gatctctacg gcaaccgcgg gaccttcgcg
     4261 cataacccgc gcatgtgctt gaaaggctat accttccatc gccgtgtgta tggcccctat
     4321 cgtttgaagg gacccttgat gcggaagggc tggaaggagt ggatggacgc gggggcaccg
     4381 gaactcacgc cggacgtcaa acggaagtac aaatttgagg cccgtttcct ggacgacatg
     4441 atgcgggtgt cctgggacac ggcggcaacc tatgcggcga agggctgtat catcatcgcg
     4501 acccggtata gcggtgaagc cggcgctcgt cggcttaggg agcagggtta tgctcctgaa
     4561 atgatcgaaa tgatgaaggg tgctggagtc cgctgcttca agcaccgtgc cgggatgccg
     4621 gtgctcggca tcatcggaaa gatgatgaat acgcgattca acggtggcgt actgccgctc
     4681 ctggattcct ggattcgcaa ggtggatcct gagcgagcgc agggggggaa atactattcc
     4741 aactatacct ggcacgggga ccaggatccg tcacatccct ggtggaacgg tacgcagaat
     4801 tgcgacatcg acctcagcga tatgcggttc agcaagctga acaccagctg gggcaaaaac
     4861 ttcgtcgaga acaagatgcc ggaagcccac tggaagctcg aatcgatcga gcggggcgcc
     4921 cggatcgtgg tggttactcc ggaatacaat ccgacggcct atcgtgccga ttactggatc
     4981 cctgttcgac cggagaccga cggggccctg tttcttagcg cctgcaagat catcgttgat
     5041 gaaaacatgc aggatatcga ttatatcaaa cagttcaccg acatgccgct gctggttcgg
     5101 acggatacat tgcaatacct ggatccgcgc gacgtcattc ctgatttcaa attcccggat
     5161 ttttcgaaga gctactccgg tcgaattcaa gcgttgaagc cggagcatat cgaacggctc
     5221 ggcggattca tggtctggga tcaggtgaag aaacaggccg tattcctcca tcgagaacag
     5281 gtcggtttcc atttcgtgca gtccggcatc gatcccgcct tgacgggaac gtaccgcgtc
     5341 aaactgctga atggccgtga agtcgacgtt tcaccgattt atcaaatgta cttggtgcac
     5401 ttccaggatt acgacctgga tacgtctcat cagattacca gggcgccgaa agacttgatc
     5461 gtgcgttggg ccagggattc gggcacgatc aagccggccg ctatgcacaa cggcgaagga
     5521 gtctgtcact atttccacat gacggaaatg ggtcgggcgg ccgcgcttgt cctgaccttg
     5581 acagggaaca ttgggaagtt cggcaccggg tgtcatacct ggtccggcaa ctacaaagcc
     5641 ggcatttgga acgccactcc ctggtcgggt gccggcttgg ctgtgcacac gggcgaggat
     5701 cctttcaatc aaaccctcga ggccactgcg catggcaagg aaatcaagac caagtcgtac
     5761 tactacggcg aagaggtcgg ctactggaac catggcgata cggccttgat cgtcaataca
     5821 cccaagtatg gacgcaaggt gttcaccggg aagacccaca tgccgagccc cagcaaggtc
     5881 cggtgggtga cgaacgtgaa cattctcaac aacgccaagc accactatga catggtgcgc
     5941 aacgtcgatc cgaacatcga gatgatcgtg acccaagaca tcgagatgac ctcggacgtg
     6001 aatcatgccg acatcgcgtt tgcctgcaac agctggatgg aattcacgta tccagaaatg
     6061 accgccacgg tctccaatcc gtggattcaa atctggaagg gtggcatcag gccgttgtat
     6121 gacactcgta acgacgccga tactttcgcc gcggtcgcgg tgaaactgac agaaatgacg
     6181 ggcgatgtcc ggatgcgaca agtgttccac tttgtgtatc agaaccgagt ggacgtctat
     6241 gcgcagcgcg ttcttgacgc cagttccacc tgttacggct acagcgcgga catcatgctg
     6301 aagtcggaga agggctggat ggttatgacg cgaacctatc cgcgccatcc gctctgggag
     6361 gaaaccaacg agagcaaacc gcaatggaca agatccggac gcatcgaaac ctatcggatc
     6421 gagccggagg cgatcgagta cggggagaat tttgtcgtgc accgcgaagg accggaagcg
     6481 acgccgtatc tgccgaacgc catcttcacg agcaacccgt acgtccggcc ggacgactac
     6541 gggattccca tcacggcgca gcaccacgac gacaagcata tccgcaacat caagctgccg
     6601 tggcaggaaa tcaagcggaa cgcgaacccg ttgtgggaga aggggtacca gttctactgc
     6661 gtcaccccga agacccggca tcgggtgcac agccagtggt cggtgaacga ctgggtgcag
     6721 atttacgagt cgaacttcgg cgatccgtac cgcatggata aacggacgcc gggggtcggc
     6781 gagcaccagt tgcacatcaa cccgcaggcg gcgaaagacc gcggcatcaa cgacggcgac
     6841 tactgttacg tcgacgggaa cccggtggac cggccgtacc gcggctggaa accgtcggat
     6901 ccgttctaca aggtggcgcg gctgatgatc cgctgcaagt acaatccggc atttccctat
     6961 cacgtgacga tggcgaaaca cgccccgtac gtgtcgacgg cgaagtcggt gaaaggccac
     7021 gagacgcgcc cggacggacg cgccatcgcg gtggacaccg gctatcaatc caacttccgg
     7081 tacggggcgc agcagtcctt taccagaagc tggttgatgc cgatgcacca gaccgacagc
     7141 ttgccgggca agcacgcgat tgcctggaaa ttcaagtggg gctatgccat tgaccaccat
     7201 gcagtaaata ccaccccgaa ggaatgtctg atccgcatca ccaaggcgga agacggcggc
     7261 atcggggcgc ggggtccgtg ggaaccggtc cggaccggct tcacgccggg tcaggagaac
     7321 gagttcatga tcaagtggct caagggcgaa cacatcaaga tcaaagtgta accgtgaggc
     7381 gtgaggcgtg aaacgtgagg cgacaggacg aagagccggc tcttctccta acaccttacg
     7441 cctaacgcct gacggaaagg agagacgcaa tgcccgaagt ctataactgg caactgggac
     7501 ggaagatgct gtatccctac gaggagcggc atccgaagtg gcagtttgcc tttgtgttca
     7561 acatcaatcg gtgcttggcg tgtcagacct gctccatggc tgacaagtcg acctggctct
     7621 tctcgaaggg ccaggaatac atgtggtgga acaacgtgga gaccaagccc tacggcgggt
     7681 atccgcagtt ctacgacgtg aagatcaccc agctcatcga gcaggtgaac ccgggcgggc
     7741 aggtgtggaa cgtgcgggtc ggccgcaagc accatgcgcc gtacggggtg ttcgaagg
//
LOCUS       DATIFR010000004         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2108056, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000004 DATIFR010000000
VERSION     DATIFR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108056"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..449
                     /gene="iscU"
                     /locus_tag="VLD60_00080"
     CDS             66..449
                     /gene="iscU"
                     /locus_tag="VLD60_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HSB43372.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKDEDGVGTGMVGAPECGDVMKLQ
                     IKVQGDTIVDAKFKTFGCGSAIASSSLATEWLKGKTIEEAQKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADTK"
     gene            472..828
                     /locus_tag="VLD60_00085"
     CDS             472..828
                     /locus_tag="VLD60_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSB43373.1"
                     /translation="MDIAKPETQAPVITLTEAALKEVKRLINVQGLTEGGLRLGVKGG
                     GCSGLSYTINFDEKIGQYDQVYEFDGVKVIVDAKSAIYLQGTQLDYHKDLMGGNFKFV
                     NPNANKTCGCGESFSA"
     gene            868..1626
                     /gene="hscB"
                     /locus_tag="VLD60_00090"
     CDS             868..1626
                     /gene="hscB"
                     /locus_tag="VLD60_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HSB43374.1"
                     /translation="MARRHACLVRTGSVMDHRHTHSINPRELQMARSMCWHCQSEVSG
                     EYFCERCVKVQPVSKETDYFTCFGFPRRLTIDPGKLEAKFYELSRAFHPDFYQNKSET
                     EQAISLGNAAMLNTAYRTLRDPIQRTEYLLDLEAGAVKEIRSSPPADLFEEILELQDT
                     LDEYRATDRASEAAQQLRTKLQAEQHMLEQRKQDMEAQLQQFFTDWDALQDRGEATSR
                     ARSERDRLLKQMREILSNRTYVKNIVNDLVATLG"
     gene            1632..>2168
                     /locus_tag="VLD60_00095"
     CDS             1632..>2168
                     /locus_tag="VLD60_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSB43375.1"
                     /translation="MARIVGIDLGTTNSLVAYMKQGTPTVIAGRTGRAMVPSVVAMTD
                     NGLIVGDAAKEHLTRNPERTVYSVKRFMGKGLADVQDELAYFPYSLAEQGGVIRIKLG
                     EKSYSPPQISAMILKELKQRAEAHLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQE"
BASE COUNT          549 a          623 c          608 g          388 t
ORIGIN      
        1 gtattgatct gaaatccgtt cagtgggctg cacactgaat cgcgcgggaa acgaaggagg
       61 aagtcatggc ctatagcgat aaagtcgtcg atcatttcaa taatccccgg aacatgggta
      121 gcttcaagaa ggacgaggat ggggtcggca cgggcatggt cggcgcaccg gaatgcggcg
      181 acgtgatgaa gttgcagatc aaggtccagg gcgacaccat cgtggacgcc aagtttaaga
      241 ctttcggctg cggctcggct attgccagct ccagcctcgc aactgaatgg ctcaagggca
      301 agacgatcga ggaagcccag aagatcaaga acacagacat cgtgcaggag ttgaacctgc
      361 ctccggtcaa gatccactgc tccgtgttgg ctgaagacgc gattaaagct gcgttggccg
      421 attaccagaa gaaggccgat acgaagtaac gactgaggaa aggagtccgc catggatatc
      481 gccaaacctg aaacccaagc tccggtgatc acgctgacgg aggcggcgtt gaaggaagtc
      541 aaacgcctca tcaacgtgca gggccttacc gaaggcggac tgcgcttagg cgtgaagggt
      601 ggcggctgtt ccgggctgag ctacacgatc aacttcgacg agaagatcgg tcaatacgat
      661 caggtctacg aatttgacgg ggtcaaggtg atcgtcgatg ccaaaagcgc gatctacctc
      721 caaggcacgc agctcgacta ccacaaagac ttgatgggcg ggaatttcaa gttcgtcaat
      781 ccgaatgcga acaagacctg cggctgcgga gaatcctttt cggcctaatg agcgacgaca
      841 gctagaggag agcatttttt cacaggcatg gcacgacgcc atgcctgtct tgtcagaaca
      901 ggtagcgtga tggaccatcg ccacacccac agcataaatc cccgcgaact ccagatggcc
      961 agaagtatgt gctggcactg tcaatccgag gtgtccggag agtatttctg cgaacgctgc
     1021 gtgaaagttc aaccggtttc gaaagagacg gactacttta cctgcttcgg atttccccgt
     1081 cggctcacca tcgatccggg caagctggaa gccaagtttt acgaactcag ccgcgcgttt
     1141 catccggact tttaccagaa caagagcgaa accgagcagg ccatcagcct cggcaatgcc
     1201 gcaatgctca atacggccta ccgcacgctc cgcgatccga ttcaacggac cgaatatctg
     1261 ctcgacctgg aagccggggc ggtcaaagag attcgctcgt cgcctccggc cgacctcttc
     1321 gaggaaattc tcgaactcca ggatacgctt gatgagtatc gggccaccga tcgcgcgtcg
     1381 gaagcagccc agcagcttcg aacaaaactc caagcggaac agcacatgtt ggaacaacgc
     1441 aagcaggata tggaagcaca actccagcag ttcttcaccg actgggacgc gctgcaagac
     1501 cgtggcgaag caaccagccg agctcgttct gagcgggacc gcctgctgaa gcaaatgcga
     1561 gagattctct ccaatcgcac ctacgtcaag aacatcgtca acgacctcgt cgcgaccctc
     1621 ggataaacat catggcacgc atcgtcggca tcgatcttgg caccacgaat tccctcgtgg
     1681 cctacatgaa acagggaacg cccacggtaa ttgccggacg aaccggccgt gccatggttc
     1741 cctccgtcgt ggccatgacc gacaacgggc tgattgtcgg tgacgctgcg aaagaacacc
     1801 tgacccgcaa cccggaacgg acggtctact ccgtgaaacg gttcatggga aaaggtctcg
     1861 ccgacgtgca ggacgagttg gcctacttcc cctattcgct tgccgaacaa ggcggggtca
     1921 ttcgcatcaa gctcggcgag aagagctatt cgcctcccca aatctcggcc atgatcttga
     1981 aggagctgaa gcaacgggcg gaagcccacc tcggcgaaag catcacgaaa gccgtcatca
     2041 cggtgcctgc ctacttcaac gacagccagc ggcaagccac gaaggacgcc ggcatgatcg
     2101 ccggcctcga agttctgcgt atcatcaacg aaccgaccgc cgcgtcgctg gcctatgggc
     2161 tccaagaa
//
LOCUS       DATIFR010000005        17152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1533264, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000005 DATIFR010000000
VERSION     DATIFR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533264"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VLD60_00100"
     CDS             complement(<1..370)
                     /locus_tag="VLD60_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43376.1"
                     /translation="MIAECVGLDIGQTAIKAVRFRRRLTGRESVEYFHLPLPYGRPES
                     TEPARRAGFLRNFLWQNGLYGSGDIVTALPCQDLFIRTLSFPFRDTAKLEQVVPFEVE
                     NLIPMALDDVAMGSMVMPVRD"
     gene            complement(439..1287)
                     /gene="gspN"
                     /locus_tag="VLD60_00105"
     CDS             complement(439..1287)
                     /gene="gspN"
                     /locus_tag="VLD60_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HSB43377.1"
                     /translation="MWVVVGTTSFLLFLLLTFPYGALQSRVLSDMTKATGWEVRAADW
                     SPGFPVAVEWNDVTWTRPGGGSVPIQVMRLNIGMLGLIMGQHTVNALVQFAGAGQTGT
                     GKASGTVSASSWSFLGPVSFKGHFHQIDLGAVAKPYVSRGLLQADIAHRWENHGQEGI
                     VFKGDGSWKIEIKELVLDRIPLGTAVVPSLSFSRVNAVLNCRDAVCDVVEFKGDGPDG
                     TVTALGRILLHPSLPASTLDLTVTVLAGAGWAQKAGNLPIPPLPPGTPLTLKLAGSVA
                     NPKLTL"
     gene            complement(1338..1958)
                     /locus_tag="VLD60_00110"
     CDS             complement(1338..1958)
                     /locus_tag="VLD60_00110"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HSB43378.1"
                     /translation="MCSRLAHSSERGFTLVEVLLATALVAVIAAMVFGSLHLSTLAID
                     SARSTASREQLLRSTLRIMADELTASVSVPVGPWMGLNSDWEGQSADTVAFVTLGQFR
                     GVESSQETELVRIVYAREGDRLVRFVRRNLYGLTDESVDQMQLAANVKAFNVRYYDGQ
                     ANVWSDDWDGRARNAAPSAVLIELTLIQDETESRTFRQWVPVGVRS"
     gene            complement(1936..2385)
                     /locus_tag="VLD60_00115"
     CDS             complement(1936..2385)
                     /locus_tag="VLD60_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspI"
                     /protein_id="HSB43379.1"
                     /translation="MEIRLDHNMFMASRLTSNHGFTLLEVLIAIAILALALPVLLGLR
                     NFDLDLHARAGDITAATLLAQEKLVEAELSPAFPLGETSGDFLKPPIGYQALGDISNR
                     ADKYRWKRIVTTTPLNAVREVKIQILWQEGMSDEVLEVSTYVFTPGT"
     gene            complement(2410..3027)
                     /locus_tag="VLD60_00120"
     CDS             complement(2410..3027)
                     /locus_tag="VLD60_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSB43380.1"
                     /translation="MRINGRGVARDPGGFTLLELIIVLFLLGGMLSLVIPRLSIGDSL
                     SSVGRRWVGALKSFQEMSMVTQKTVRLYVDIDRGMYWPMVLEGNQEKIPLDPTWATPI
                     SLPESIRFTDIQVGAATKQSGRTELFFYPNGRIDQATMHLADADNNIMGILIEPTTAL
                     IRVSDQRIEPPKPRTIPDRIRPLLQPTLQPTTAGKPLPLPSGVTK"
     gene            complement(3047..3547)
                     /gene="gspG"
                     /locus_tag="VLD60_00125"
     CDS             complement(3047..3547)
                     /gene="gspG"
                     /locus_tag="VLD60_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSB43381.1"
                     /translation="MGVHTRAERISKRLLQSERGFTFIEIMVVVAILAILAALVVPRI
                     MGRTDEAKRTAAKVQIRNIEGALQLYKLDNGVYPSTEQGLKALVEKPSVGVIPKKWKL
                     GGYLPSLPEDPWGNPYKYLSPAPLQQGQYGQMKGDYEITSLGTDGEAGGEGINADITN
                     WNLDKD"
     gene            complement(3573..4811)
                     /gene="gspF"
                     /locus_tag="VLD60_00130"
     CDS             complement(3573..4811)
                     /gene="gspF"
                     /locus_tag="VLD60_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HSB43382.1"
                     /translation="MPVYHYRGYRADGGSATGIIDAESPKVARLKLRKDGVFPTEMAE
                     QEGASKSITAYSGRSPIAGVGRSATLTVNDVALLTRQMGTLLVAGLPLVDSLGVLLDQ
                     SDKKGIKSLLADVREAIRGGASYSAVLERYPKDFSPIYVHMVRAGEASGALDQILFRL
                     AEFLEKQLALKNKVTNAILYPAIMLVVGTLVLFFLVTFVVPKITAVFEHSKQALPWPT
                     VALMEISHFCRDYWMVLLGLVLGSIWMVRRTLRTEAGRTMADRILLRIPLIGQVARMV
                     SISRLASTLATMLSSGVQLLDALDVSKRVMNNRVLEQAVEEARQNIREGETIAEPLKR
                     SGEFPPLVTHMIGVGEKSGEMEEMLRRIGQIYDGEVDRVITRFTSLLEPVMIVVMGVV
                     VLFIVVAILLPIFEMGQMVR"
     gene            complement(4816..6546)
                     /gene="gspE"
                     /locus_tag="VLD60_00135"
     CDS             complement(4816..6546)
                     /gene="gspE"
                     /locus_tag="VLD60_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HSB43383.1"
                     /translation="MAKQPHITRPLLGAILEQKFGLSEAKLLEALNVQQTKGGRLGEA
                     LLRIRAIEEDLLLQALAIQFEMPWLPHLEVSQVDHELIRKVPIHFARRYRVLPLKVED
                     GAIVVATTDPLETAALDDLRLLLGMPVKAVLTSGVSLMSCLNRAYDEVASPTGAEQVM
                     EDIAASENLDQLAHELDEPQDLLDATDEAPIIRLVNSVLFQAVRQRASDIHFESFERG
                     LVVRYRIDGVLYPILTPPKHLQSSIIARLKIMAGLNIAEKRLPQDGRFAIRTAGKDVD
                     LRVSVLPTSHGERVVLRLLEKENRLLNLSEMGFSPDHLRTIQQLIQLTHGILLVTGPT
                     GSGKTTTLYAALSEINAPDKNIITVEDPVEYQLLGIGQMQVNPKINLTFAAGLRSILR
                     QDPDVIMIGEIRDRETAEIAIHASLTGHLVFSTLHTNDAASAATRLIDMGIEPFLVAS
                     SVVAVLAQRLIRKVCPDCKKAYQPEDEELIRLGVVPGRNRPTFYRGTGCPACSQTGYR
                     GRTGIHELLVMDDEIRRLIGSKADSTSIKHTAIAKGMVMLKEVAVEKIFAGVTTTEEV
                     MRMTQQEVEV"
     gene            complement(6780..7640)
                     /locus_tag="VLD60_00140"
     CDS             complement(6780..7640)
                     /locus_tag="VLD60_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43384.1"
                     /translation="MTSRRALILFVVIGTTFLIAHTINAVIAEALFVPTGISSPQALV
                     QPEVAADRSPLALAEQIRTSGLFPLPANPIGMAGSEPGAVRQTMNVASKIKLLGVVVG
                     DREGVSAIVEELASKRQLFFRLHDRIPDIGEISEIRRGGILIRQGDQQELLALSTGLP
                     DKPPSAQVASASPATSTGGGSIRKALDRREVEQAMNDLPKLLSQARAVPVLTNGAMTG
                     FRLDYIAPGSFYEKIGLQYGDTLKQVNGVEIKDPGTMLSLFQQLRNEQSVKLDVLRNN
                     QRTTMTFDIR"
     gene            7909..8916
                     /gene="gmd"
                     /locus_tag="VLD60_00145"
     CDS             7909..8916
                     /gene="gmd"
                     /locus_tag="VLD60_00145"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSB43385.1"
                     /translation="MKKALITGITGQDGSYLSEFLLKRGYQVYGIIRRSSSFNTGRID
                     PIYEDPHVPQRRLHLIYGDLNDASSLNRIIRTVQPDEIYNLGAQSHVRVSFDIPEYTG
                     EITGLGTIRLLEAIRESGLKPKFYQASSSEMFGKVLEVPQRETTPFYPRSPYGAAKVY
                     AYWITVNYREAYNLFACNGILFNHESPRRGETFVTRKITKAAARIKLGLQQDLYLGNL
                     DAKRDWGFAGDYIEAMWMMLQVDQPDDYVIATGETHTVREMLELAFDRLQLDWKKHVK
                     IDEKYYRPTEVDLLIGDAGKAKRQLGWTPKVGFQELIAMMVDADLAAERERLEGTRGK
                     A"
     gene            8919..9881
                     /locus_tag="VLD60_00150"
     CDS             8919..9881
                     /locus_tag="VLD60_00150"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HSB43386.1"
                     /translation="MRGFWSDTRVVVTGGAGFLGSFVVEKLRARGCSEIVVPRSRDYD
                     LVRMEDVRRLYADARPDIVIHLAARVGGIGANQANPGRFYYDNLMMGAQLIEVGRQQG
                     LRKFVAIGTICAYPKFAPIPFKEDDIWNGYPEETNAPYGLAKKMMLVQSQAYRQQYGF
                     NSIVLFPVNLYGPRDNFDLETSHVIPALIRKCAEAKQAGRPDIVLWGDGTPTREFLYV
                     EDAAEGILEAAEHYNESHPLNLGTGEEVSIKNLARIIGAEVGYLGHIRWDTTKPNGQP
                     RRCLDISRIKQTIGFQPKHSLREGLQKTIHWFQEHRTSLREVHF"
     gene            10079..10594
                     /locus_tag="VLD60_00155"
     CDS             10079..10594
                     /locus_tag="VLD60_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB43387.1"
                     /translation="MPDLTVPKTVPTDITREQGIDGAEQYERGITLRKAGLFKQAIEE
                     LDQAARDRAYALKAYAQIGLCYKSSGRYEEAVLAFRNALKSPQASRKETVQILYVLGR
                     TLESLGRIAETLEAYRWIRREDPDYRDVENRITQLSTKRISVKRSAVSTQRSWVGGVI
                     QSWQDRLRASK"
     gene            complement(10623..14318)
                     /locus_tag="VLD60_00160"
     CDS             complement(10623..14318)
                     /locus_tag="VLD60_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB43388.1"
                     /translation="MSSSSASRPPNRLIHETSPYLLQHAYNPVDWYPWGPEALRLARE
                     KDRPILLSIGYSACHWCHVMEHESFENEAIAALMNRHFLCIKVDREERPDLDEIYMAA
                     TVAMNHGQGGWPMTVFLTPDQEPFFGGTYFPPEDRWGRPGFGTLLKKIAEVWEKNSSD
                     LRTQARELTARLKREWQAPVPVSISAEVLDEAANAFSREFDSRFGGFGSAPKFPPAAG
                     LSLLLRCYRRSGDTESLQMVTKTLDAMAAGGIYDHIGGGFARYSTDERWLVPHFEKML
                     YDNALLVRTYLEAYQVTKQPSYRHVVIDVVEYILREMTDSEGGFYSATDADSEGVEGK
                     FFVWTPSEVRAVLQNDEDARRICACYEITEQGNWEHTNIPNRMRPIESIAQELDVTVD
                     ELQDTMRRVRPLLYEARKQRVPPALDDKVITAWNGMMISAMAEAGRVLGISRFVDAAR
                     RAADFLLRVHRTAEGRMHRTSRKGHAHLDAVLEDYAYFGEGLVDLYEACGNECYLAAA
                     QELAARVLEAFQDRDQGGFFTTSEQHELLILRGREGADGATPSGNAVAASLLARLSFH
                     YDRRDFREAATAAVRAYGKQMARYPRAFAKSLAVVDFLTEGPLELAFVGKKDDPGLAA
                     LWQATCEVFLPNRIIAVTNRVDGTSSHPLLTGRGLVNGQAALYLCRNFACQQPLTHPL
                     AAEKALRTTATQIAQGSRGTRLQGRLLAGYATSEGTARYAVRLVNQPRISGKFEHGYV
                     SLGSTGLTTSKLGFGCYRVDGSDPEQGAALAKALQEGVNLVDTSTNYMDGESERLVGF
                     LLRDLMAKKALAREEVIVVSKIGYVQGHNLKAAEAREQAGRPYPEMVKYGEGIWHCIH
                     PEFLADQLDASLDRLGLETLDICLLHNPEYFLSEAAHHGGSNVAELRDEYYRRIAQAF
                     GYLESQVAAGRIRWYGVSSNTVTSVATDVDATSLSRLLEAAGKAAASQGHQRHHLAVL
                     QCPMNLFESGALTTANTGFEQKDTVLQQARREQVAILVNRPLNAMPMRSAGVWRLTNF
                     LLDGDPVDIEEQLRIVGILENEYRTEIAPAIPHSGEGMAPVDFFNWSAELAQIRLRLQ
                     GLEHWDQIEHQMIAPHVNQVLQAIPRLLNGTAAERWEAWRDRYIPPLLTLLRGLRRES
                     TEKSQIRTGKIMVAVDPLLPAHRQMESLSRKALWVLASTPGVTCVLNGMRTSRYVDDS
                     LAVLGWEPLKEPASVYERVKHVVESAG"
     gene            complement(14454..14681)
                     /locus_tag="VLD60_00165"
     CDS             complement(14454..14681)
                     /locus_tag="VLD60_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43389.1"
                     /translation="MKRMLMAIMAVAVAVTFSAPAFAGEEKKDKKDEKKGGHVLVYGE
                     EKKDKKDEKKGGHADTFSEKKDEKKDEKKGK"
     gene            14877..16319
                     /locus_tag="VLD60_00170"
     CDS             14877..16319
                     /locus_tag="VLD60_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HSB43390.1"
                     /translation="MNDIGPYSNYRLTVRLELANKPGMFAKVAATLAEEEANLGAVDL
                     VSATADRMVRDVTFDVRDEAHGDRVLKRLAGIPQVTVLSASDRIFLLHLGGKIQVQSK
                     LPISTRNVLSMVYTPGVGRVSQAIAKDKTKAYAFTTKSNSLAVVTDGSAVLGLGNLGP
                     EAALPVMEGKSMLFKELAGIDAWPICLGTQDPDEIVRTVQAIAPGFGAINLEDIGAPR
                     CFDIERRLKASLDIPVMHDDQHGTAVVLLAALTNALTVLGKHIEHVRIVVNGLGAAGT
                     ACCRMLLAAGASHLLGCDKEGIILSGEADQLRACRTDLSSCLTPHRPKGSLRDALKGA
                     DVFIGLSVPNVLTADDLDLMAPDRIVFAMANPDPEVSPALAISHCRIFATGRSDFPNQ
                     INNALAFPGIFRGALDVQASEINESMKLAAARALAETIPRAALSEDYIIPSVFDKEVV
                     PRLARAVAAAARETGVTRRRPKFSEERPPS"
     gene            16488..17078
                     /locus_tag="VLD60_00175"
     CDS             16488..17078
                     /locus_tag="VLD60_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSB43391.1"
                     /translation="MQKRIGSLRSKTEDSMDVAVDSMMQERVDSYFRVEEGLEEIVKS
                     LAQIEEELEQVRDLSGAMRLESRLEFVEDRWDDLDSEIRERPRRRRRKISLADMLKAA
                     SGGGGDLSQSSGSVNNAVDAYAIMGVEFGSSLADVTAAFRQKAKLLHPDANNGDRRME
                     PELRRMLDAYQFLKEYLSLSNTEPMRPPDRPYSPAE"
BASE COUNT         3860 a         5002 c         4557 g         3733 t
ORIGIN      
        1 agtccctgac cggcatgacc atactgccca tcgccacgtc atccaatgcc atcggaatca
       61 gattttcgac ttcaaacgga acaacctgtt caagttttgc agtatcccgg aaggggaagg
      121 aaagtgtacg gataaagaga tcctggcagg ggagcgccgt cacaatgtct ccgctcccat
      181 agaggccatt ctgccagagg aagttgcgca agaatcctgc gcgacgagcc ggctcggtgc
      241 tctccggtct cccatagggg agagggagat gaaaatattc gacggattcc cgaccggtca
      301 aacgccgacg gaaccgaaca gccttgatgg ctgtctgtcc gatatcgaga cctacacact
      361 cggcgatcat ggcatcatca ctcggttgtc acgcttgcag cggcggcccg tcgaggcctg
      421 tcgcacagaa tggctatttt acaaggttag cttgggattt gcgactgatc cagccagttt
      481 caaggtgagg ggtgttcccg ggggaagagg ggggatcggt agattcccgg ctttctgagc
      541 ccatccagcc ccggccagca cggtcaccgt gaggtctaac gtgctggctg gtaacgacgg
      601 gtggagaagg attcgtccca aagccgtaac cgttccatca ggtccatcgc ctttaaattc
      661 aacgacatca cagacggcat cccggcaatt cagcaccgca ttaacccgac taaaactgag
      721 ggaaggaacg actgccgtcc cgagaggaat ccggtctaag accagttcct tgatctcgat
      781 tttccatgat ccgtctcctt tgaagacaat gccctcctga ccgtgattct cccatcgatg
      841 agcgatatcg gcctgtaaca atcctcggct cacatagggc ttcgctaccg cgcctaaatc
      901 aatttgatgg aaatgcccct tgaaagacac aggcccgaga aacgaccagg acgacgcgct
      961 cacggttcca cttgctttcc cggttcccgt ttgaccggcc cctgcaaatt gcaccagcgc
     1021 gttcacagta tgctgaccca tgatcaaccc aagcattccg atgttcagac gcatcacctg
     1081 gatgggaacg ctcccgcctc ccggtctggt ccaggttaca tcgttccact ccactgcgac
     1141 cggaaatccc ggcgaccaat cggccgcacg tacttcccac ccggtggctt tcgtcatgtc
     1201 cgagagaact ctcgactgca gagccccgta gggaaacgtg agcaacaaaa acaggagaaa
     1261 gcttgtagtc ccaaccacga cccacatgag cggctctttt ttgaggtcag gaactgatgg
     1321 ccaattcagc ctcccgatca tgatcgcacc cccaccggca cccactgacg aaaggtccgc
     1381 gactccgtct catcctggat gagggtgagc tcaatcaaca cggcgctcgg cgccgcgttc
     1441 cgcgcacgtc cgtcccaatc atccgaccag acgttggctt gcccgtcgta atatcgaaca
     1501 ttgaaggcct tcacgtttgc cgccaactgc atttggtcga cactctcgtc ggtgagcccg
     1561 tagaggttgc gccggacgaa acgaaccagt cggtcgcctt cccgtgcata gacgatccgc
     1621 acaagttccg tctcctgact ggattccaca ccgcgaaatt gtcccagcgt gacaaaggcc
     1681 acggtgtcgg ccgactgacc ttcccaatct gaattcaagc ccatccaggg gccgacgggg
     1741 actgaaacgc tcgcggtcag ttcatccgcc atgatgcgca gggtgcttct gagaagctgt
     1801 tcccgcgaag cggtgctgcg tgcgctgtcg atcgccaggg tgctgaggtg caatgatcca
     1861 aataccatgg cggcaatcac cgccacgagc gcggtggcca gcaacacctc taccagggtg
     1921 aatcctcgct cagagctatg tgccaggcgt gaacacatag gtgctgacct cgagcacttc
     1981 gtccgacatg ccttcctgcc acagaatctg tatttttact tcccgcaccg cattcagcgg
     2041 tgtcgtggtg acgatgcgtt tccaacgata cttgtctgcg cgattggaga tatcgccgag
     2101 cgcttgataa ccaatcggag gcttcaggaa atcaccgctg gtttcaccga gcggaaatgc
     2161 cgggctcaac tccgcctcga ccagcttttc ctgagccagc agagtcgcgg cagtgatatc
     2221 gccggctctc gcatgcaagt ccaggtcaaa attccgtagt cccaacaaca ccggcagagc
     2281 caacgcaaga atcgcgatcg cgatcagcac ctcaagcaac gtgaatccgt ggttgctcgt
     2341 tagtcgggaa gccatgaaca tgttatgatc caatcggatt tccatcgtac cagtccttcg
     2401 tcaggcgcct tatttcgtga caccggatgg aagaggcaga ggcttccccg ccgtggttgg
     2461 ctgaagcgtt ggctgaagca agggtctgat tcggtcgggg atcgtccttg gtttcggcgg
     2521 ttcgatgcgc tgatcggaca cccgaatcag cgccgtggtc ggctcgatga gaattcccat
     2581 aatgttgttg tcggcatcag ccagatgcat ggttgcctga tcgatgcgcc cgttcgggta
     2641 gaaaaacagt tccgtccgcc cggattgttt tgttgcagcg ccgacttgta tatccgtaaa
     2701 ccgaatcgac tcgggaaggc tgatcggtgt ggcccaggtc ggatcgagtg gaatcttttc
     2761 ctgatttcct tccagcacca tcggccagta cattcctcgg tcgatgtcga catacaaccg
     2821 aaccgtcttc tgagtcacca tcgacatctc ttgaaatgac ttcaacgcac caacccagcg
     2881 acgcccgaca ctgctcagac tgtctccgat cgacagacgg ggaatgacca ggctcaacat
     2941 cccgcccagc agaaacagaa caatgatcaa ttcgagcaag gtaaatccgc ctggatcgcg
     3001 cgcaactcct ctaccattga ttcgcatagg ctgcaggccg cttcccttaa tctttgtcga
     3061 gattccaatt cgtgatatcg gcgttgatac cttcgccgcc ggcctcgcca tccgtgccca
     3121 atgacgtgat ttcgtaatcg cccttcatct gcccatattg tccctgttgg agcggtgccg
     3181 ggctgagata cttgtaggga ttgccccagg gatcttcggg cagactgggg agatagccgc
     3241 cgagcttcca tttcttcggg atcacgccga ccgagggttt ctccaccaga gcttttaatc
     3301 cttgttctgt cgaaggatag acgccgttgt caagcttata gagttgcaag gcgccttcga
     3361 tgttgcggat ctggaccttc gccgccgtgc gtttggcttc gtcggtacgt cccatgatcc
     3421 ggggtacgac cagcgctgcc agaatcgcca aaatggcgac aacgaccatg atctcgatga
     3481 acgtgaatcc ccgttcagat tgcaggagcc gcttactgat tcgctccgcc cgcgtatgta
     3541 cacccaccgg ctgcctcctt cgattcaaac gattaccgca ccatctggcc catttcgaaa
     3601 atgggaagca agatggccac cacgatgaac aacacgacca cgcccatcac cacaatcatg
     3661 accggctcga gcaaggaggt aaatcgtgta atgactcgat cgacttcccc gtcatagatt
     3721 tgaccgattc gccgtaacat ttcctccatc tcaccgcttt tctcaccgac accgatcata
     3781 tgggtgacca gcggaggaaa ctctccgctt cgcttcagcg gctcggcaat cgtttctccc
     3841 tcgcggatat tctgtctggc ctcttccacg gcctgctcga gcacgcgatt gttcatgacg
     3901 cgtttcgaga cgtcgagcgc atcgagcaac tgcacgccgc tcgacagcat cgtcgccaac
     3961 gtgctggcca gccttgagat ggacaccatg cgggcaacct gaccgatcag gggaatcctc
     4021 aacaagatcc ggtcagccat ggtccgaccg gcttccgtcc gcagcgtccg gcgaaccatc
     4081 cagatgcttc ccaggacgag ccccaagagc accatccagt agtcgcggca gaagtgacta
     4141 atctccatga gtgcaacggt tggccatggc agcgcctgtt tcgaatgctc aaacaccgct
     4201 gtgattttcg gcacgacaaa tgtcacgaga aaaaacagca cgagcgtccc caccaccagc
     4261 atgattgccg gatagagaat cgcattcgtc actttgttct tcagggcgag ctgcttctca
     4321 aggaattcgg caaggcggaa caaaatctga tccagcgcgc cgctggcttc gccggcacga
     4381 accatatgca catagatcgg cgagaaatcc ttgggatagc gttcgaggac cgcactgtac
     4441 gacgcgccgc cacgaatggc ttcccgcacg tcagccagca ggctcttgat ccctttttta
     4501 tcagactgat ccaacagcac gcccagcgag tccacgagcg gaagacctgc gacaagcagc
     4561 gtgcccattt gcctggtcaa taaggcgacg tcgttcacgg tgagcgtagc ggagcggcct
     4621 actccggcaa tcggactcct gccggaatat gctgtaatcg atttgctcgc cccttcctgt
     4681 tccgccatct cggtggggaa cacaccatcc ttgcgcaatt tgaggcgagc aactttcgga
     4741 ctctcggcat cgatgatacc ggtggcagag ccgccgtccg cacggtatcc cctgtagtgg
     4801 tagaccggca tggcgtcaga cttcgacctc ttgctgcgtc atgcgcatga cttcttccgt
     4861 cgtggtcacg ccggcaaaga tcttttcgac agcgacttct ttgagcatga ccattccctt
     4921 ggcgatggcc gtatgcttga tcgatgtgga atcggccttg ctcccgatca gccggcggat
     4981 ctcgtcatcc atgaccagga gttcatgaat ccctgtgcgg ccgcgatacc cggtttggga
     5041 acaggccgga caaccggtcc cccggtagaa cgtgggcctg ttcctgcccg gcacgacacc
     5101 caaccgaatc agttcctcat cctcaggctg atacgctttc ttacaatccg ggcacacctt
     5161 tcgaatcagt cgttgcgcga gtactgcgac gacagaagac gccacgagga acggctcgat
     5221 tcccatgtcg atcaatcgag tggccgcgct ggctgcgtcg ttcgtgtgca gcgtcgagaa
     5281 gaccagatgc cccgtcagcg aggcatggat tgcaatctcg gccgtttcac ggtcgcgaat
     5341 ttctccgatc atgatcacat cgggatcttg ccgaagaatc gaccggagtc ccgccgcgaa
     5401 ggtcagattg attttggggt tcacctgcat ttggccgatg ccgagcagtt gatactccac
     5461 cggatcttcg accgtaatga tgttcttgtc aggcgcgttg atctcgctta atgccgcgta
     5521 cagtgtggtg gtcttcccgc ttcctgtagg gcccgttacc agaagtatcc cgtgcgtcaa
     5581 ttgaatgagc tgctgaatcg tgcgcagatg atccggtgaa aagcccatct cggacaggtt
     5641 cagcaagcga ttttccttct ccaacagccg caagaccacc cgttcaccat gcgaggttgg
     5701 taatactgac acacggagat ccacatcctt accagccgtg cgaatggcga agcgtccgtc
     5761 ttgggggagc cgcttttcag cgatgttcaa gccggccatg atcttgagac gggcaatgat
     5821 actggactgt aaatgcttcg gtggggtcaa gatcgggtag agcacgccgt caatccgata
     5881 ccggaccacc agcccccgtt cgaaggattc aaaatggata tcacttgccc gctgtcgcac
     5941 ggcttggaag aggaccgaat tgaccagtct gataatgggc gcttcgtccg tcgcatccag
     6001 caggtcttgc ggctcgtcga gttcgtgcgc gagttggtcc aaattttcac tggcggcgat
     6061 gtcctccatc acttgttcgg caccggttgg gctggcaact tcatcatagg cccgattcag
     6121 gcagctcatg agagacacgc cgctggtcag gactgccttc accggcatgc ccagcagaag
     6181 gcgcaaatcg tcgagcgcag cggtttccaa cgggtcggtg gtggccacga cgatggcgcc
     6241 gtcttccacc ttcaagggaa gaacgcgata gcggcgggcg aaatggatag ggactttccg
     6301 gatcaactca tgatcgacct gactcacttc aagatgcgga agccacggca tttcaaattg
     6361 gatcgccaac gcctggagaa gcagatcctc ctcgatcgca cgaattctga gcaaagcttc
     6421 tcccagtcgg cctcccttcg tctgctggac attgagggct tcgagcagtt tggcttccga
     6481 caaaccgaat ttttgctcga gaatcgcccc gagcaacggt ctggttatgt gaggttgttt
     6541 agccacattc ctagtctttg tctatcgaac caacttccaa ggccatgttg gccttcggac
     6601 ggggaggagg gaccacgtaa catatagaaa tataataaat tactcctctc ttgccaacct
     6661 tgacaagcaa caagaagagc ggccgccaag gccacaacct cgcaaccaaa gctctcggga
     6721 tgggaaaaaa cttgaatggg ggttagagag gggtcccccg gcaccggcaa gcgaccggct
     6781 tatcgaatat cgaacgtcat ggtcgtgcgc tgattattgc gcagaacatc cagcttaact
     6841 gattgctcat tgcgaagctg ctgaaagaga gacagcatcg ttccgggatc cttaatctca
     6901 accccgttga cctgcttgag cgtgtctccg tattgcaggc cgatcttttc ataaaagctg
     6961 cctggggcaa tgtaatccaa gcggaagccg gtcatcgctc cgttggtaag cacgggaacc
     7021 gctcgggcct gcgagaggag cttgggaaga tcattcatcg cttgctcgac ttctcgacga
     7081 tcaagggctt tgcgaatcga cccacctccg gtggatgttg ccggagaggc cgaggcaact
     7141 tgagccgagg gaggtttgtc cggcaggccc gtggaaagcg ccagcaattc ctgctggtca
     7201 ccctggcgaa tgaggattcc cccgcgccga atctcgctga tttctccgat gtccggtatc
     7261 cggtcgtgca atcggaagaa gagctgtcgc ttgctggcaa gctcttcgac aattgcagaa
     7321 accccttctc ggtcacccac gacgacgccg agcaatttga tcttggaggc tacgttcata
     7381 gtttggcgaa ctgcgccggg ttcagatccc gccattccga tcggattggc gggaagcgga
     7441 aacagtccgc tcgttcgaat ctgttcggcc aaggccaagg gggaacgatc agcagcaacc
     7501 tctggctgaa ccagggcctg cggcgatgag atgcctgtcg gcacgaacaa cgcctccgca
     7561 atgactgcat tgatcgtatg agcgatcaaa aacgtcgttc ctatgacaac gaagaggatg
     7621 agcgctcggc gcgaagtcat gataatggca ccttagttgg acccacttcc cagctggtgt
     7681 tgcattgtaa ggcggcaacc ggcggggtgc aatagatgtc atttccttgt ccttgcatca
     7741 cacggccagg ggcctacaag ccgacgtccc accgttccgt ggctcgatgc ccgagcagga
     7801 tgggttcagc ctaccaaggc cccgactaat aagagttgag ataatcgagt cagtggacat
     7861 tcctcgggat tcgatccata atttccgcac aacaaaagga gattcgttgt gaaaaaagca
     7921 ctgatcaccg gcatcacggg gcaagacggt tcctatctct cggaatttct tctcaaaagg
     7981 ggctaccagg tttatggcat tattcgacgc tccagttcct ttaataccgg ccgcatcgat
     8041 cccatctatg aagatccgca cgtgcctcaa agacggctgc acttaatata tggcgatctc
     8101 aacgatgcca gttcgctgaa ccgcatcatc cgaacagttc agccggatga gatttataat
     8161 ctcggtgcgc aaagtcacgt tcgtgtgagt tttgacattc ccgaatacac cggagagatc
     8221 acgggtcttg gcacgattcg tcttctcgaa gccatccggg agtctggatt gaagccaaaa
     8281 ttctatcaag cttcatccag cgaaatgttc gggaaggttc tcgaagtgcc tcagcgggaa
     8341 accactccct tttatccccg cagcccctat ggggcggcaa aagtctatgc ctactggatc
     8401 accgtcaact atcgcgaagc gtataatctg tttgcctgca atggcattct cttcaatcac
     8461 gaatctcccc ggcgtggtga aacgttcgtc acgagaaaaa ttacgaaagc cgccgcgcgt
     8521 atcaagctcg gtctccagca ggatctttac cttggcaatc tggacgccaa gcgggactgg
     8581 ggatttgccg gggattacat tgaggccatg tggatgatgc tgcaggtcga ccaaccggac
     8641 gactatgtga tcgcgaccgg cgaaacccat acggttcgag aaatgctcga actcgcattt
     8701 gatcgtctgc aactcgactg gaagaaacac gtgaaaatcg acgagaagta ttaccgcccc
     8761 actgaagtgg acctgctcat cggagacgcc ggcaaagcca agcgccaact gggatggacg
     8821 ccgaaggttg gattccaaga attgattgcc atgatggtgg acgccgatct ggccgcggaa
     8881 cgggagcgat tggaaggcac gagggggaag gcgtaatcat gcgaggcttt tggagcgata
     8941 cacgcgtggt cgttaccggt ggggcaggct tcctcggttc cttcgttgtc gagaaacttc
     9001 gagcaagggg ctgctcggag atcgtcgttc ccaggagccg agactacgat ctcgttcgga
     9061 tggaagacgt gagacgcctc tatgccgatg ccagaccgga tatcgtcatt catctggccg
     9121 cacgcgtggg aggtatcgga gccaaccagg ccaatcctgg gcgattttac tatgacaatc
     9181 tgatgatggg cgcccagctc atcgaagtcg gccggcaaca gggactccgg aaattcgtgg
     9241 cgatcggtac aatctgcgcc tacccgaaat ttgcgcccat tccattcaaa gaagacgaca
     9301 tatggaacgg atacccggaa gaaaccaacg cgccgtacgg gctcgccaag aaaatgatgc
     9361 tggtccagtc tcaagcctat cgccagcaat acggcttcaa ttcaatcgtc ctattcccgg
     9421 tcaaccttta cggaccgcgt gataattttg atttagagac ttcccacgtc attccagcct
     9481 tgatcagaaa atgcgctgag gccaagcaag ccggtcggcc tgacattgtc ctgtggggtg
     9541 atggaacgcc gacgcgagag tttctctatg tggaggatgc cgcggagggt atcctggaag
     9601 ctgcagagca ttacaacgag tcacatcctc tcaatcttgg aacaggagaa gaagtgtcga
     9661 tcaaaaacct cgcccgcatc attggtgcag aagtcggata tctaggtcac attcgttggg
     9721 ataccacaaa accaaatggc cagccacgtc gctgcttgga catcagccgt attaaacaaa
     9781 ccattggctt ccagcccaag cattcactcc gcgaaggact tcagaaaacc attcattggt
     9841 ttcaagagca tcgcacctcc ctccgtgagg tacacttctg acgtaaccgg tagcagcccg
     9901 cctcccagct cttactcttc attgacgacc gatggacgaa atatgcacgg tccggttcat
     9961 tctctctacg gtatgtcgag acaatcatgc ctaactcgtt gacagttcag aacctgcgaa
    10021 gcatgttcaa gtggcccatg ttttgctaga agactaatac taaggagtga ccagcaccat
    10081 gcctgacctt accgttccta agaccgtccc cacagatatc acgagggagc aagggatcga
    10141 tggagcggag cagtacgagc ggggcatcac gctgaggaaa gccggcttgt tcaagcaagc
    10201 cattgaagaa ctcgatcaag cggccagaga ccgtgcctat gccctcaaag cctacgcgca
    10261 aatcggtctc tgctacaagt cgagcggtcg ctatgaagaa gccgtgctgg ccttccgaaa
    10321 tgccctcaaa tcgccgcagg catcgaggaa ggaaaccgtt cagattctat atgtgctcgg
    10381 gcgaacgctc gaatcgctgg gacgcattgc cgaaacgctg gaagcctatc gatggattag
    10441 gcgtgaagat ccggactaca gagatgtgga aaaccgtatc acgcagttga gcacaaaacg
    10501 aatatccgtg aagagatccg cggtctcaac gcaacgatcc tgggtcggtg gggtcatcca
    10561 atcttggcaa gatcgccttc gtgcctccaa gtgagaagaa cccgcaactt gagaggcaga
    10621 atttaaccgg ctgattctac aacgtgcttg acccgctcat agacagaggc cggttctttc
    10681 agcggttccc atcccaagac cgcaagcgaa tcatccacat aacgactggt tctcatcccg
    10741 tttaagacac acgtcacgcc gggagtactt gccaacaccc agagcgcctt cctcgacaat
    10801 gattccatct gtcgatgtgc cggcagaagg gggtcgactg ccaccatgat ttttcccgtg
    10861 cggatctgac tcttctcggt cgattcacga cgcaatccgc gaaggagcgt aagcagaggt
    10921 ggaatatacc gatcccgcca agcctcccat cgttcggcag ctgttccgtt gaggagccga
    10981 gggatcgcct gcaacacttg attcacatgt ggcgctatca tttgatgttc gatctgatcc
    11041 caatgttcca atccctggag acgaagcctg atctgtgcca attctgctga ccagttgaag
    11101 aaatccaccg gcgccatccc ttctccgcta tgcgggatgg ccggggcaat ctctgtccga
    11161 tactcgtttt ctaatatgcc aacgattcgg agctgttcct cgatgtcgac cggatcccca
    11221 tcgagaagaa agttcgtcag tcgccatact ccggcgctcc gcatcggcat cgcgttgaga
    11281 gggcgattaa ccagtattgc cacttgttcc cgtcgggcct gttgcaatac ggtgtccttc
    11341 tgctcgaatc ctgtattcgc tgtcgtcaac gctccggact cgaaaagatt catgggacat
    11401 tgcagtacgg ccaagtgatg tcgttgatgc ccttgcgatg ccgcagcttt ccctgctgcc
    11461 tcgaggaggc gagacaacga tgtggcatca acatccgtcg caacgctcgt caccgtattc
    11521 gaggaaactc cgtaccaacg gatgcggccc gctgcaacct gggactctag atagccaaaa
    11581 gcctgcgcga tcctacggta atattcatcc cgcaattcag ctacgttgga tccgccgtga
    11641 tgtgccgctt cggacagaaa atattccgga ttgtgtagca agcagatatc gagcgtttcc
    11701 aatcctaagc gatccaacga cgcgtccagt tgatcggcca agaactcagg atggatgcaa
    11761 tgccaaatac cctcaccgta cttcaccatc tcagggtaag gtcggccggc ctgttcgcgc
    11821 gcctccgctg ctttcaaatt gtgcccctgc acgtacccga tctttgaaac cacgatgact
    11881 tcttcacggg caagggcctt ctttgccatc aagtcacgca gcaagaaacc gaccaggcgt
    11941 tcgctctcgc catccatata attggtcgat gtgtcgacga gattcacacc ctcttgaagg
    12001 gcctttgcaa gggcggcacc ctgctctgga tcacttccgt ccacacgata acagccgaat
    12061 cccaactttg atgtcgtcag tcctgtcgaa cccaacgaca catagccatg ttcgaacttc
    12121 ccggatattc gtggctgatt caccagacga acggcgtacc gtgcggttcc ttcactggtc
    12181 gcgtatccgg caagcaaccg cccctgcaat ctcgtcccac gagagccctg agcgatttgc
    12241 gtagctgtag ttcgaagcgc cttttccgct gccaagggat gcgtcaacgg ttgttgacac
    12301 gcgaagtttc ggcaaagata gagggcggct tgaccgttta ctagtcctct cccggtgagg
    12361 aggggatggg aggatgtgcc gtcaacccgg ttcgttacgg caatgattcg attaggcaag
    12421 aacacttcac aagtcgcttg ccagagtgca gccaacccag gatcatcctt cttcccgaca
    12481 aacgcgagct cgagcgggcc ttccgttaaa aaatcgacga cggcgagact ttttgcgaat
    12541 gctctcggat atcgcgccat ctgttttccg tacgcgcgga ccgccgccgt cgccgcttct
    12601 cgaaaatccc gacggtcata atgaaacgag agtctcgcca agagggaggc tgccacggca
    12661 ttgccgctcg gcgtcgcacc atcggcgccc tctcgcccac gcaggatgag caattcatgt
    12721 tgttccgatg tggtaaaaaa ccccccctgg tcgcggtcct gaaacgcctc gagcactcgc
    12781 gccgccagtt cctgtgcagc cgcgagatag cactcgtttc cgcaggcctc gtaaagatcg
    12841 acgagtccct ctccgaagta ggcatagtct tccaaaacgg catcgagatg cgcatgccct
    12901 ttccgagagg tccgatgcat gcgaccctca gcggttcgat gtacgcggag aagaaagtct
    12961 gccgcgcgac gggccgcatc gacgaagcgc gatatgccca atacacgacc ggcctcggcc
    13021 atagccgaaa tcatcatgcc attccaggcg gtaatgacct tgtcgtccaa ggcaggaggc
    13081 acccgttgtt tgcgtgcttc ataaagcagg ggacgaaccc gccgcatcgt gtcctgtagt
    13141 tcatcaaccg tcacatccaa ctcctgcgcg atcgattcga tcggtcgcat tcgattggga
    13201 atattcgtat gctcccagtt tccttgctcg gtgatctcgt agcaggcgca gatgcggcgg
    13261 gcatcttcat cgttctggag aacagcacga acttcgctgg gggtccagac aaaaaactta
    13321 ccttctacgc cctccgagtc ggcatcggtt gcagaataaa accctccctc ggagtcagtc
    13381 atctcccgca aaatgtactc caccacgtct atgacgacgt gacgatatga cggttgtttg
    13441 gtgacctgat acgcttcgag ataggttctg acgagcaacg cattgtcgta gagcattttc
    13501 tcgaagtgcg ggaccagcca ccgctcatcg gtcgaataac gagcaaaacc tcccccgatg
    13561 tggtcgtaga tcccgcccgc ggccatggca tccagcgttt tcgtcaccat ctgcaacgac
    13621 tcggtgtcgc ctgatctgcg gtaacatcga aggaggagtg agagaccagc tgccggagga
    13681 aatttcggtg cggagccgaa tcctccaaaa cgggaatcga attcccggct aaaagcgtta
    13741 gccgcctcgt cgagaacctc agcgctgatc gaaacaggga cgggagcctg ccactcgcgt
    13801 ttcaatcgcg ccgtgagctc acgggcttgc gtgcggaggt ctgatgaatt tttttcccac
    13861 acttcggcaa ttttcttcaa taatgtgccg aatcctggcc gtccccatcg gtcctctgga
    13921 gggaaatatg tccccccaaa aaaaggttct tgatccggcg tgagaaacac ggtcatcggc
    13981 caaccgcctt ggccgtgatt catcgccacg gtcgcagcca tgtagatttc atcgagatcc
    14041 ggtcgttctt cccgatccac tttaatgcag agaaagtggc ggttcatgag cgcggcgatc
    14101 gcctcattct caaatgactc atgctccatc acatgacacc aatgacaggc cgaatagccg
    14161 atagaaagca ggatgggacg gtccttttct ctcgcaagcc tcaaggcctc cggtccccag
    14221 ggataccaat cgaccgggtt ataggcatgc tgaaggagat agggactggt ttcgtgaatg
    14281 agccgattgg gagggcgtga cgcggatgat gacgacatga tgtcacctta gcatccgaca
    14341 gaaacgagga tcaacggacc aaaaagaaga gcctatggtc cgaagaccat aggctcttcc
    14401 tcaattcgaa gctggagctg gaatcgagtg ggagtcgaga cggggtttcg aaattacttg
    14461 cccttcttct cgtccttctt ctcgtccttc ttttcgctga acgtgtccgc gtgtccgccc
    14521 ttcttctcgt ccttcttatc cttcttttct tcaccgtaca cgaggacgtg gccgcccttc
    14581 ttctcgtcct tcttgtcctt tttctcttcg cccgcgaacg ccggggcgct gaaggtcact
    14641 gcaactgcaa ctgccatgat ggccatcaac atgcgcttca tagttcccta ccccctatag
    14701 aaattgtgat gtgatgtttg cctgaccatc aggcaaacgg cggatacggt atcgaaatgt
    14761 tccctgtctt gtcaatccga gattctttcg ttcgggagta ttcgactcga cattcttccc
    14821 tcaaaaacac acccttgtgg tatggttaag gcaatctcgc cgtctgagtg gttcgtatga
    14881 atgacatcgg tccatactca aactatcgcc ttacagttcg tctcgaactt gcgaataaac
    14941 ctggcatgtt tgcaaaggtt gctgccacat tggcggaaga ggaggcgaat ctaggcgccg
    15001 tcgacttagt atcggccaca gccgaccgca tggtacgaga cgtcacgttc gacgtgcggg
    15061 atgaagccca tggggatcgg gttctcaagc gcttggcggg aattccgcaa gttaccgtcc
    15121 tctccgcttc ggaccgtatt ttcctcctcc accttggggg aaagatccag gtccagagca
    15181 aactccccat ttccacgcgc aatgtcctct caatggtcta tacccccggt gtcgggcggg
    15241 tgtctcaggc aattgccaaa gacaagacga aagcctatgc ctttaccacg aagagtaaca
    15301 gcctcgccgt cgtcacggac ggatctgccg tgctgggtct cggaaattta gggcctgagg
    15361 cggcacttcc tgtcatggaa gggaagtcca tgctctttaa ggaactggcc gggatcgatg
    15421 cctggccgat ctgccttggc acccaagacc ccgatgaaat cgttcgaacc gtgcaagcca
    15481 tcgccccggg attcggcgcc attaatttag aagacatcgg cgctccgcgc tgttttgaca
    15541 tcgaacgacg gctcaaagcc tcgctcgaca ttccggtcat gcatgatgat caacatggca
    15601 cggcggttgt cttgctggca gcccttacga atgcgctcac ggttctggga aaacatatcg
    15661 agcatgtccg gatagtggta aacggcttag gtgccgctgg aaccgcctgc tgccgcatgt
    15721 tgctggccgc cggcgcctcc catctgctcg ggtgcgacaa agaaggtatc atcctctctg
    15781 gagaggcaga ccagcttcgc gcctgtcgga cggacctttc ctcttgcctc acgccgcatc
    15841 gaccgaaggg tagcttgcgg gatgcgttga aaggcgccga tgtgttcatc ggcctttccg
    15901 tccccaacgt gttgacggct gacgacctcg acctcatggc accggatcgc atagtttttg
    15961 cgatggccaa ccctgatccc gaggtgtcac ccgcactcgc gatttcgcat tgccgcattt
    16021 tcgcaaccgg gcgatccgac tttcccaacc aaatcaataa cgccttggca ttcccgggca
    16081 tcttccgcgg cgcactcgac gtacaagcat ctgaaatcaa cgaatcaatg aaactggcag
    16141 cagcgagagc cctggctgag acgattcctc gagccgccct cagtgaggac tacattatcc
    16201 cgagtgtatt cgacaaagaa gtcgttcctc gcttggcacg tgccgttgca gcagcggccc
    16261 gcgagaccgg tgtcactcgc cggcgaccga aattctctga agagagaccg ccgtcctagc
    16321 caatcctcgc gcgatgggct tatcctgatt cgaccacgaa acgcgaattc cctcgattct
    16381 cccttctggc tcccgacagg gctgtgctac aatgccatcg cgttatccta agcgtgtgga
    16441 tcccgatgcc attttcaacc agcaagttca tgaaatttcg gagtgggatg cagaaacgga
    16501 ttggctcgct ccgttcaaag actgaagaca gcatggatgt ggccgtggat agcatgatgc
    16561 aggaacgggt cgacagttat ttccgagtcg aagagggcct ggaagaaatt gtgaagtcac
    16621 tggcccagat cgaggaagag cttgagcaag tccgagatct ctccggcgcc atgcgattgg
    16681 aatccaggct cgaattcgtc gaagatcgct gggacgatct ggatagtgaa atcagagaac
    16741 ggccccggcg gcggcgacgc aagatcagtc tcgctgatat gttgaaggca gcgagcgggg
    16801 gaggggggga cctttcccaa agctccggaa gtgtcaacaa tgcggtggac gcttacgcga
    16861 ttatgggagt ggaattcggc agctcgctcg ctgatgtcac tgcggccttt cgacaaaagg
    16921 caaaactgtt gcatcctgat gccaataatg gcgatcgccg tatggaaccg gaactgcgcc
    16981 gcatgcttga cgcctatcaa tttctcaaag aatacttgag cctgagcaat accgaaccga
    17041 tgcgcccgcc agatcgtcca tatagcccag ccgagtaacc agtggcagcc acacctcctt
    17101 ctcacaacag ctacatcacg agccgccgcg aacttgaatc actttgcgaa cg
//
LOCUS       DATIFR010000006         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2108982, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000006 DATIFR010000000
VERSION     DATIFR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108982"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..527
                     /gene="spoVG"
                     /locus_tag="VLD60_00180"
     CDS             222..527
                     /gene="spoVG"
                     /locus_tag="VLD60_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HSB43392.1"
                     /translation="MDVTEVRVFPVNNEERLKAYATITIDNVFLIRDLRVINGNAGLF
                     VAMPSRKMKDGTFKDIAHPLNSETRKLIEQKVLAEYERELANPTKREQAPKDGETKQ"
     gene            683..756
                     /locus_tag="VLD60_00185"
     tRNA            683..756
                     /locus_tag="VLD60_00185"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:715..717,aa:Gln,seq:ttg)
     gene            779..1732
                     /locus_tag="VLD60_00190"
     CDS             779..1732
                     /locus_tag="VLD60_00190"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSB43393.1"
                     /translation="MVKLRGQEIKIFSGNANIALAKEICQRMNLPLGNAVVGRFSDGE
                     INVQIVDNVRGMDVFVVQPTSSPVNRHLMELLIMIDALKRASAARITAVLPYYGYSRQ
                     DRKVQPRVPITSKLVADLITTAGANRVLTVDLHAGQIQGFFNIPVDNLFAAPVLLDYL
                     KSKKFNNLTVVSPDAGGVERARAFAKRLGSALAIIDKRRDKPNVAQVMHIIGDVKGRD
                     CLVLDDMVDTAGTLTEGAGALKANGASNIYAACTHAVLSGPAIQRLNESSLAELITTN
                     SISLDSKRQECKKLTVLSVAPLLAEAITRIHEETSLSSLFV"
BASE COUNT          421 a          497 c          555 g          381 t
ORIGIN      
        1 cggatgagcg gaagcgggcc gacggtcttc ggcatctttg agaccgagtc cgcttgccgg
       61 gctgccgcat caaacctctc gagcacgggg tggcggctct tcccggcgga aatactgacc
      121 gaatcgccgt ttgcggggca cctggtgtaa acgagggcgc cgtgggtgtg acggcgcgca
      181 cctccatcag aaagcaaggt gacaccacga ggaggtagca tatggacgtt accgaggtga
      241 gggtatttcc ggtcaataac gaagagcggc tcaaagcata tgcgaccata acgatcgata
      301 acgtgttttt gattcgtgac ctgcgtgtga taaacgggaa cgccggcttg ttcgttgcaa
      361 tgccgagccg gaaaatgaag gacgggacgt tcaaagacat cgcccatccg ctgaacagcg
      421 agacgaggaa gttgatcgaa cagaaggtcc ttgcggaata cgagcgggaa ttggcaaatc
      481 ccacgaagag ggagcaggct ccaaaggacg gcgagacaaa gcagtaattg aagcttcccg
      541 gggcaggctc cgggaatggc tcacttccag aattaccatg gtaagttttg ccgattctga
      601 atcgatgcga gaggataggc acgcgcctat ccttttcatg tcttatccgg ttgtcgaacc
      661 ttgtgcggct ttggctcccg attcggggat cgtctaatgg taggacagag gcctttgaag
      721 ccttttatcg gggttcgact ccctgtcccc gaaccagtgt gctgaaagaa ggggtctgat
      781 ggtgaaacta cgaggtcaag agatcaagat tttttccggg aatgccaaca tagcgctggc
      841 aaaagagatc tgccagagga tgaaccttcc gctcgggaac gctgtggtcg gccggttttc
      901 cgacggagag atcaacgtgc agatcgttga caatgtgcgc ggcatggatg tattcgtggt
      961 gcagcccacg tcaagcccgg tcaaccggca cctcatggag cttctaatca tgatcgacgc
     1021 gcttaaacgg gcgtcggccg cgcggatcac ggctgtgctt ccctactacg ggtacagcag
     1081 acaggaccgg aaggttcagc cacgcgttcc gatcacttcg aagttggtcg ccgatctgat
     1141 cactacggcg ggcgccaacc gtgtgcttac ggtggacctg cacgctggac agatccaggg
     1201 attcttcaac atccccgtgg acaatctttt tgccgcgccc gtgctcctcg actatctcaa
     1261 gagcaagaag ttcaacaacc tgacggttgt atcacccgat gccggaggcg ttgaacgcgc
     1321 gcgggccttc gccaagcggc tcggctcggc actcgcgatc atcgacaagc ggagggacaa
     1381 gccgaatgtc gcccaggtca tgcacatcat cggtgacgtc aaaggtcgcg actgcctggt
     1441 cctggacgac atggtggaca ccgccggcac cctcacggag ggggcggggg cgctcaaggc
     1501 taacggggca agcaacatct acgctgcctg tacgcatgcg gtgctctccg gtccggctat
     1561 ccagcggctg aacgagtcct cgctcgccga gttgatcaca acgaactcca tctcgctcga
     1621 cagcaaacgc caggaatgta aaaagctgac ggttctctcc gtcgcgccgc tcctcgcgga
     1681 agcgattacg aggatccatg aggagacgtc gctcagttcg ctgttcgtgt aataaaagtt
     1741 caccacgaag acccaaagaa gttcttcaag atcctgtttt tctttgtgtc ctcgtgtctc
     1801 tgtggtaacg ttgacgcatg atgctttatt aatgtatcgg aggagaaatg gaaa
//
LOCUS       DATIFR010000007        17317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1343855, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000007 DATIFR010000000
VERSION     DATIFR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17317
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343855"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VLD60_00195"
     CDS             complement(<1..1004)
                     /locus_tag="VLD60_00195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSB43394.1"
                     /translation="MATEIIMPQLGESIAEGTIVKWLIPVGGVIQKDQSLLEVETEKV
                     TLEIPSPATGVLSEIIVQQGETVPVGTLLARIENAPASEVINRVGGVVIRPMEPALEG
                     EDYHSPAVRQLAKESGVDLSQVKGTGAGGRVTKKDVQDYITQGGSPVKAASPAGEHSM
                     GEDILPFTQMRKTIADRMVKSRQTSAHVTTFFEADFTGITKFREGRNLTYLPFVIRAV
                     TRAAREMPVVNSSWSEQGIVIKKDIHIGIATALENGLLVPVVRDADRKGLTQLAKEIA
                     DLAERARTKKLSPEEVQGGTFTITNHGGFGSLFSTPIIHQPQVAILGVGAIQKRAVVV
                     "
     gene            complement(1090..2082)
                     /locus_tag="VLD60_00200"
     CDS             complement(1090..2082)
                     /locus_tag="VLD60_00200"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSB43395.1"
                     /translation="MTTATAQSEVTYLEAISQALDEEMTRDERVFLMGEDIGAYGGAF
                     KITEGFLQKYGEWRVIDTPLAESGFVGAAIGAAMMGLRPVVEMQFADFISCAFDQITE
                     VAAKNHYRWGAAVPMVIRAPFGGGVHGGPFHSECPEGWFFHSPGIKLVAPSTPYDAMG
                     LLKAAIRDPNPVIYFEHKFLYRRIKATLPQDDYIVPIGKADVKRAGNDLSIITYGAMV
                     HLALEAAQVLANEGIDVEVVDLRTLIPLDKDAVYGSVRKTSKAILLHEDNKTGGIGAE
                     IAALLAEDCFDCLDGPIIRIAPPDTPVPFSTPLEEFFLPKTKDIIAAARKLAAY"
     gene            complement(2079..3080)
                     /locus_tag="VLD60_00205"
     CDS             complement(2079..3080)
                     /locus_tag="VLD60_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSB43396.1"
                     /translation="MDLADVTREIKRADLLQMYYYLRLTRALEDRVTALYRQGRIVGG
                     CYTSHGMEAIAVGYASALERDDVIAPFHRDMGAFLVRGFTPGEVLAQYLGKRTGPTKG
                     KDGNVHMGDLKRGVIGFVSHLADNLPVATGVALAFKIRGESRVAFAGTGDGGTSRGDF
                     HEAMNFAAVRKLPVVLFCNNNQYAYSTPQRLQMAVSDVVDRAKAYGMPGEIVDGNDVA
                     AVYLASKRAILKARAGEGPTFLEFKTMRMHGHSEHDSAKYVPRELLEEWKKKDPILKA
                     ERLLTQLGYGDEAYFHEIGERAKKEVEAGLEFAEQSPLPEGPEVLEGVFATADEVES"
     gene            complement(3113..3865)
                     /locus_tag="VLD60_00210"
     CDS             complement(3113..3865)
                     /locus_tag="VLD60_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43397.1"
                     /translation="MSVTMVEQALAELVERHQKLYDGPAIDTKLEPVVRAGSHTGFTN
                     RDVLRVLRMSRSVFFETHVEVVSGHHTATYLRFESIARFPELIKQLAQDMAEWIGRTF
                     HHDPITGIVATTSAAELLARGIADQLHDTMRLRVALTPYSCETGRIGTDITSGAIKSG
                     ERFLALNDVTTRGNCVSKLGKVITDNGGLLAGMMVFARRDSGQFPLMNELVARYPFYW
                     TTDLDMPQWEPTICPLCRMNKPLLTWKDMPEL"
     gene            complement(3862..4860)
                     /locus_tag="VLD60_00215"
     CDS             complement(3862..4860)
                     /locus_tag="VLD60_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSB43398.1"
                     /translation="MKLVSFRITTPVGTFTRVGATHRQNVVDLNMAYTRWLGDQREAQ
                     PYRLANAQVPSTMLEFLEGGSSTMAAARRAFDYVVTLGPSTKGPFGETVFYGTSEVKT
                     TAPLPNPTSLRDFIAFEDHIAATSRKRGQPIPPEWYKAPVYYKGNHRTIIGPEEDLSW
                     PLHTKKLDYELELACVIGRKGEDIPEQEAQDYIAGYTIMNDFSARDIQFQEMACRLGP
                     AKGKDFATALGPCLVTPDEIADLGALTMVARVNGEEWSRGRFGTIHWSFPQMIAHVSR
                     GETIYPGDVFGSGTVGGGCGLEMDRYLNPGDVVELEVQPIGVLRTRVVGATRREGV"
     gene            complement(4857..5966)
                     /locus_tag="VLD60_00220"
     CDS             complement(4857..5966)
                     /locus_tag="VLD60_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HSB43399.1"
                     /translation="MYLNKKGKVPNQAHVGIPEGLFEEEHGREGFAGPVSHLYRTHPP
                     TGWVKIDGPLKPRAFTCTQLSTTSPSHATDQQTLVMQSQDVRLHVSYRAETMRYFVRD
                     ADGDEVYFVHQGRGRFETDYGTLSYEPGDYVVIPKGTTYRTHIDAGPSLFLVIETPEP
                     ITVPDRGLLGQHALFDKGVLVAPELGTVEPPETQGQEWEVRIKRQGDSTRVIYPFYPM
                     DVMGWKGDLWAAKLNVRDFRPVTSARYHLPPSVHVTFRAGGCLISTFVPRPLESDPES
                     LRVPFYHRNTDYDEVLFYHQGEFFSRAGIQPGMLTLHPQGIHHGPQPQAVQASKGKTQ
                     TSEVAVMIESKTPFTVAPEMESVELKDYALSWSRR"
     gene            complement(5995..6582)
                     /locus_tag="VLD60_00225"
     CDS             complement(5995..6582)
                     /locus_tag="VLD60_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB43400.1"
                     /translation="MDQTKGIDHITLCVEDLDAAEFLFTKILGFEVIWSARDVGSDKS
                     SMDTVVVQRGAARVALMQGRDKTMKSQITEFIEKYGQGVQHFALEVDDIQAVCREWET
                     RGVKFSGPLKEGRDGFGPLKQRFTHPLFQSGGLFIELTQRAHGTEKAKTFVRSTVEAL
                     YKDIERDQASGVERTIIDYQSPTVPITKKTMKQAS"
     gene            6915..7646
                     /locus_tag="VLD60_00230"
     CDS             6915..7646
                     /locus_tag="VLD60_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSB43401.1"
                     /translation="MTFYQEMRSVVLGHGAINNSYLDRFQRGNLTDAELKEFAIEFYS
                     FARFFPQILVSQLVNTEDENVADELTKVLYSELGDGQTKHRHELLYRDFLRSIGIDVH
                     EAMSRPMLPSTRAYIEGMERLYSDGNHAKALGASFGLENMAITMWDCLIPGLTSLKST
                     NYSRMDMTYFTFHRELESTHEEAMEHAVAALQGTTSDGMSDQEKADFRCGMNAVLGYL
                     EGFWMGLERSGSPDPEPQRSHSHAA"
     gene            7694..8656
                     /locus_tag="VLD60_00235"
     CDS             7694..8656
                     /locus_tag="VLD60_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB43402.1"
                     /translation="MSEIAKYLEAIKERFDLSSDYALAEKLGIAQPEANLMRRGLKIP
                     KPELCIKIAKLLNKNPVELLLIAQKDKAPSSAKEYWTLALTAVDVMLHVPKTPKYIPR
                     KVEAIGRELRQLETQTLTYQGAEANAEAVRLVQTAENSIDAIMERWNIWKKGEALYPS
                     YLLANQEAANRGVVIRRLLVLTREQLRQESVVADAIQVMDDQQRAGIKIFFAFREELE
                     RAPAFQRFEEDYEAQGAAKDLNAAIFDREILIFSQSYGQVQLGVVGVPTALTMINQLE
                     ITWKPDLLRDLDPAPLFDMTRYVFEYGGPQAFNMELARFKRSVA"
     gene            8653..10059
                     /locus_tag="VLD60_00240"
     CDS             8653..10059
                     /locus_tag="VLD60_00240"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HSB43403.1"
                     /translation="MKFPSEPFKIKVVEPIRRTTREERDRLLCGAGYNLFQLPAESVY
                     VDLLTDSGTSAMSDHQWAGLMLGDESYAGSKNYYHFEKTVRSIFGYKHVIPTHQGRMA
                     ENLLFSTVVKPGMCVPNNIHFDTTRANIEHLGAQALDLVVKEAYDPSCDLPFKGNMDL
                     VRLEDTINRVGRSCIPLVMLTITNNSGGGQPVSLGNIRQTRVLLSRYRIPLFFDACRF
                     AENCFFIKEREPGYAGKSVFDIAQELFSYGDGCTMSAKKDGLVNIGGFLSLNDDQWAQ
                     DITNMLILVEGFPTYGGLAGRDLEAMARGLREVLDEDYLSFRVGQVRYLGELLDQAGV
                     PILKPIGGHAVYLNAKEFLPHIPQDHFPAQSLAISLYRDYGIRGVEIGTVMFGKKDAA
                     TGRTIHPALEMVRLAIPRRVYTNMQITYVAESIIELYRRREGIQGLTLTYEAPMLRHF
                     TARFEEIQHRPLLQQTAS"
     gene            10070..11992
                     /locus_tag="VLD60_00245"
     CDS             10070..11992
                     /locus_tag="VLD60_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSB43404.1"
                     /translation="MATLFKGLEQIEEAREHVTGHGFMAGVYVGRPDFSLLLQPEESP
                     EQKAAWERYHPLLETFLRTHVDPDEIERTAKIPDSVLKGLFALGAFGMKIPTEYGGLG
                     FSYTNYGRALMLMASWSNILALTVAVPQSIGIAMPILLFGSEEQKHKYLPLVAREAIS
                     AFALTESNTGSDAANIRTEAVLDSSGTAFVVNGEKLWCTNGSIARYVTLIARVPAKRL
                     IHNGRPLWVPVPEGKGADEQVHTAFVLDMETPGVLVRQRCQFEGCRGIENAHMTFAGA
                     RIPAANVIGEVGRGLKYALTILNVGRAISVPAICLGMAKQAWQPTLDRANQRLTFQKP
                     LSERQTQRMRLGHMAAGLFAMEALASTVWRMADQHTYDVRIEAAIAKIFCSEQTIQFL
                     KDAQIIFGGMGYETAESKRFRGEPTFSVEQLVRDAEMYRIGEGATDVLRPFVAREGLN
                     THLERARNYVDERVAGLRRLTELRHLLRFYIPWYARQWVRRPLPARSEFQHPQVRPKL
                     AFVERASRRLARAIFYAMLFHRQALQDDQGRQNRIEIIGEDLLVIAAITLYAERQEKI
                     AGHPEIWDLAEEVFREGKKDIDQNIRELICNRDELVTAVGQRALSGTYPSLSDGIIRR
                     GLQDYLFRQESERKAI"
     gene            12218..13216
                     /locus_tag="VLD60_00250"
     CDS             12218..13216
                     /locus_tag="VLD60_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSB43405.1"
                     /translation="MRAWLMDSYDGIERLRLGEVPDPQPGPGQVLLRMRFAALNPADA
                     FLAQGMYPTKPPLPHILGRDGVGDVVAMGAGVNNVRMGEAVGILRCDIGVNVWGTLAE
                     KVMVPAENVIRIPSGWSLEEMAGAPLVFLTAWQALTQWNDPPAPPHTGSVLLVTGASG
                     GVGVASVLLGQSMQLTVVALSRSAEKRVRLKTLGADFVFDPADRNLIKAVMAAISPKK
                     VDLAVDSVGGALLPQVVAMLGYGGRISVVGRSGGIVPEFNTATLLFRRIRMGGVSVGD
                     ETAQAAHMVWKEIVARLDSMGQRPQVDATVSFEDVKKGFARLAQGPMGKVLVQVAA"
     gene            complement(13734..14405)
                     /locus_tag="VLD60_00255"
     CDS             complement(13734..14405)
                     /locus_tag="VLD60_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB43406.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKDDINVVGEAQDGMEAVEK
                     TRTLLPDVILMDVSMPRMGGVEATRQIKREFPHIGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMAQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANSLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LDPRP"
     gene            complement(14392..15501)
                     /locus_tag="VLD60_00260"
     CDS             complement(14392..15501)
                     /locus_tag="VLD60_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSB43407.1"
                     /translation="MPTVHAKPSKRMRRNGATGATHVAIIGAGRGGTALMEIFATDPL
                     VQIVGVAECNPSAPGLALAKRLKIPVTRDYRRLLDLERVDLVIDVSGNAEVWQVLQDF
                     HRMGVTIIGGASAKFMWELIEARIRATAEIEKTLNKYQSLYRLYVKETGAAVTEERTR
                     IACEIHDGLVQSLAGVNFKLELCQQLIRKNPKASLATLRESKEQLKLAIQEARQVIFN
                     LRPLQYDKMELIPGLTNYLKSYQTQYHIKTEFEVSGDEQILFPRTKIFLFRIVQEALS
                     NVVQHAKADRVSVRLEIQFDRLTVTIKDNGVGFDMEHVMQDPEKWDHFGIRGILERAK
                     LVGGEAKIESKKGRGTTIVVEVPLGKKENSDNGKD"
     gene            complement(15596..16060)
                     /gene="nrdR"
                     /locus_tag="VLD60_00265"
     CDS             complement(15596..16060)
                     /gene="nrdR"
                     /locus_tag="VLD60_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSB43408.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGEVIRRRRECLGCKRRYTTYERV
                     EEILPVVVKKDGRRETFDRSKILSGLKKACEKRPISTATIEAVTDRIEKRIQEMGETE
                     IESRVIGEELMKELHQLDQVAYVRFASVYREFKDIDQFMDELKSLAQQRRER"
     gene            complement(16090..>17317)
                     /gene="glyA"
                     /locus_tag="VLD60_00270"
     CDS             complement(16090..>17317)
                     /gene="glyA"
                     /locus_tag="VLD60_00270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSB43409.1"
                     /translation="ATDPEVYAAIDAEEERQRNKLLLIASENFASPAVLAAQGSLMTN
                     KYAEGYPGKRYYGGCQHVDTVEDLAIQRCKEIFGADHVNVQPHSGSQANMAAYLSVLK
                     PGDTILGMDLAQGGHLTHGSKVNFSGMIFRAFSYGVDRQTETINYEAVQKLAEECRPR
                     MIVVGASAYARTLDFPKFQEIAKSVGAYLMVDIAHIAGLIAAGLHPNPVPYADFVTTT
                     THKTLRGPRGGVTMCKAEHAKAVDKFVFPGMQGGPLMHVIAAKAVAFKEALSPSFKRY
                     QQQVVANAKVLAQGLLDRGYKIVSGGTDTHLMLVNLTNKGLTGKEADAALDAAGIIVN
                     KNAVPYDEKPPAIASGIRLGTPIVSTRGMKEAEMKEIVALVDRVLQHRQDPAILEEVR
                     AKAKALCNRFPIFHPY"
BASE COUNT         3597 a         4980 c         4838 g         3902 t
ORIGIN      
        1 ttgacgacca cggcccgctt ctggatcgct ccgaccccca gtatggcaac ctgcggctgg
       61 tgaatgatcg gcgtgctgaa gaggctgccg aatccaccgt ggttcgtgat cgtgaatgtc
      121 cctccctgca cctcctcggg actcaatttc ttggtcctgg cccgttcagc caaatccgct
      181 atttctttgg ctaactgcgt caatcccttt cgatctgcat cccgcaccac tggaacgagg
      241 agtccattct ccaacgccgt ggcgatgccg atatgaatat ctttcttgat gacgatgcct
      301 tgttcactcc aggacgagtt cactaccggc atctctcgag cggcgcgtgt caccgcacgg
      361 atcacgaagg gtagataggt caggttgcgg ccttctcgaa atttggtgat gcccgtaaag
      421 tctgcttcaa agaacgtagt gacatgggcg gaagtctgcc tgctcttgac catccggtcg
      481 gcaatggtct tgcgcatctg tgtgaaggga agaatgtctt cgcccattga gtgctcgccg
      541 gcaggacttg ccgctttgac cggacttccg ccttgagtga tgtagtcctg aacatctttc
      601 ttcgtgactc gcccgccagc tcccgttcct tttacctgcg agagatcgac tccggattct
      661 tttgctagtt ggcggacggc aggggaatga taatcttcgc cttcgagagc cggttccatg
      721 ggacggatga ccaccccgcc gactcggttg attacttcgg aggcaggagc gttctcgatt
      781 cgtgccaata gtgttcccac gggaaccgtc tcgccctgtt ggacgataat ttcgctcaac
      841 actcccgtcg ccggtgaagg aatttctagt gtcactttct cggtttccac ttccaagaga
      901 gattggtcct tttgaatcac ccctccgacc gggatgagcc atttcacgat cgtcccttca
      961 gcgatgcttt ccccaagctg cggcatgatg atttcagtcg ccacagtaac tccgtgaggc
     1021 gttaggcgtg aaacgtgagg cggtaggaaa ctcctcctta tgcctgcccc ctcacgcctt
     1081 acgcaaaaat caatacgctg ccaacttccg cgctgccgca ataatgtctt ttgtcttcgg
     1141 aagaaaaaac tcttccaagg gcgtactgaa cggaactggt gtatccggcg gagcgatgcg
     1201 aatgatcggt ccgtccaaac agtcgaaaca atcttccgcc agcagtgcgg cgatctcggc
     1261 tccgatcccg ccggtcttgt tatcctcatg caataggatg gccttgctgg tcttgcgcac
     1321 cgatccgtag accgcatctt tgtcgagggg aatcaacgtg cgaagatcga ccacttccac
     1381 gtcgattcct tcgttcgcca agacctgagc ggcctccaat gccagatgaa ccatcgcgcc
     1441 ataggtgatg atggagagat cgttcccggc acgtttgacg tcggcctttc cgatcggaac
     1501 aatgtaatcg tcctgcggca gcgtcgcttt gatccgccgg tagagaaact tgtgttcgaa
     1561 gtaaataacg gggttgggat cccgtatggc cgctttaaga aggcccatcg catcgtatgg
     1621 agttgagggg gccacgagct tgatccccgg tgagtggaag aaccatcctt ccggacattc
     1681 ggaatggaaa ggaccgccgt gcacgccgcc gccgaaggga gcgcgaatga ccatcggcac
     1741 cgcggcgccc caacgataat gattcttggc cgcgacctca gtgatctggt cgaacgcgca
     1801 ggagatgaaa tcggcaaact gcatttcgac aacggggcgc aggcccatca tcgcggctcc
     1861 aatggcagct cccacaaagc cggattcggc taatggtgta tctatgactc gccactcgcc
     1921 gtatttctgg agaaagcctt cggtgatctt gaaggcaccg ccataggcgc cgatgtcttc
     1981 gcccatcaga aatacccgct cgtcgcgggt catctcctcg tccaaggcct gcgagatggc
     2041 ctcgagatac gtgacttcgg attgggcggt ggctgtggtc atgattccac ctcatccgcg
     2101 gtggcaaaga ctccttccag gacctcgggt ccttccggca gcggactctg ctccgcaaac
     2161 tccaggccgg cctcgacttc tttcttggcc cgctcgccga tttcatgaaa atacgcctca
     2221 tcgccatagc cgagttgagt gagcagtcgt tcggctttca ggatcggatc tttcttcttc
     2281 cattcttcca gcagttcacg cggcacatac ttggccgagt catgctcgga atggccgtgc
     2341 atccgcatgg ttttgaactc gagaaatgta ggtccttcgc ctgcgcgggc ctttaggatg
     2401 gcgcgcttcg aagccagata gaccgcagcg acgtcgttcc cgtccacgat ctcgccgggc
     2461 atgccgtaag ccttggcccg atcgaccaca tcagagaccg ccatctgcaa gcgctgtggc
     2521 gtcgagtagg catattggtt gttgttgcag aacaatacga cggggagttt gcgcactgcc
     2581 gcaaagttca tcgcctcatg gaagtcgccg cggctggttc caccgtctcc ggtccccgcg
     2641 aacgcgacac gagattcgcc tcggatcttg aacgccaggg caacgccggt tgcaaccggc
     2701 agattgtcgg ccaagtggct gacgaagccg atcacgcccc gcttgagatc ccccatgtga
     2761 acattgccgt ctttcccttt agtcggtcca gttcgtttgc cgaggtattg ggcaagaact
     2821 tctccgggcg tgaatccgcg aacgagaaag gctcccatgt ctcgatgaaa gggggcgatg
     2881 acatcatctc gctccagcgc ggaggcgtag ccaactgcga tggcctccat cccgtggctg
     2941 gtgtagcacc cgcccacgat gcgtccttgc cggtagagcg ccgtcactcg atcctccagg
     3001 gccctggtga gccggaggta gtagtacatt tggagcagat cggctcgctt gatctccctt
     3061 gtcacgtctg ccagatccat gtatccctcc tcgcagagtg atggaacgag cgttagagtt
     3121 cgggcatgtc tttccaggtt agcaacggtt tgttcattcg gcacaaagga cagatcgtcg
     3181 gctcccattg cggcatatca agatccgtcg tccagtagaa gggatagcgc gcaaccaatt
     3241 cgttcatcag cgggaactgg ccgctgtcgc ggcgcgcgaa gaccatcatc ccggccagga
     3301 gccccccgtt gtcggtgatc actttcccga gcttgctcac gcaattgcca cgtgtggtga
     3361 catcgttgag cgccaggaat cgttcgccgg atttgatcgc gccgctcgtg atgtccgtcc
     3421 cgatccgccc cgtctcgcag ctatagggcg tcaatgccac gcgcaaccgc atcgtatcgt
     3481 gaagttgatc cgcgatccct cgggcgagta actctgcggc tgaggtggtg gccacgatgc
     3541 ctgttatcgg atcgtgatga aacgtccgcc cgatccactc ggccatatcc tgggccagtt
     3601 gcttaatcag ctccgggaaa cgagcgatgg attcgaaccg cagatatgtg gccgtgtgat
     3661 ggcccgacac cacctccacg tgagtctcga agaacaccga acgggacatt cggaggacgc
     3721 ggaggacatc ccgattggta aacccggtat gtgaaccggc tcgaacaacc ggttcgagct
     3781 tggtatcgat cgccggtccg tcgtagagtt tctggtgccg ctccaccaac tcggcaagag
     3841 cctgctcaac catggtaacg ctcatacccc ttccctcctg gtcgcaccca ccaccctcgt
     3901 cctcaggacg ccgataggct gaacttccag ctcgaccacg tctcccggat tcaagtagcg
     3961 atccatttca aggccgcacc ccccgcctac ggttcccgag ccgaacacgt cgcccggata
     4021 gatcgtttcg ccgcgagaga catgtgcgat catttgcggg aaggaccagt ggattgtccc
     4081 gaaccgtccc cgcgaccatt cttctccgtt taccctcgcg accatcgtca atgcgccgag
     4141 atctgcaatc tcatcaggag tgacgaggca cgggcccaag gccgtcgcaa aatctttgcc
     4201 tttcgccgga cccaaccggc aagccatctc ttgaaactgg atatcgcgcg cgctaaagtc
     4261 gttcatgatt gtatagccgg caatgtagtc ttgcgcttcc tgttcaggga tgtcttcgcc
     4321 tttgcgtcca atgacacagg ccaattccaa ctcatagtcc aacttcttcg tgtgcaaagg
     4381 ccaggacagg tcttcctccg gcccgataat ggtccggtga ttccccttat aatagacggg
     4441 tgctttgtac cattccggcg gaatcggctg gccccgcttc cttgaggtgg cggcaatgtg
     4501 gtcttcaaaa gcgatgaagt ccctgagcga agtaggattt ggaagcggag cggtagtctt
     4561 aacctcgctg gtcccgtaaa agaccgtctc gccaaacggg cctttggtcg aaggaccgag
     4621 cgtgaccaca tagtcgaacg ctcgtcgggc ggccgccatt gtcgacgatc ctccttcgag
     4681 gaactccaac atggttgaag gcacctgagc attggccaaa cggtacggct gcgcttcgcg
     4741 ttggtcaccc aaccaccgcg tataggccat gttcaggtcg acaacgttct ggcggtgagt
     4801 cgcacccacc ctcgtgaatg tgccaacagg agttgtgatc cggaaactga ccagtttcat
     4861 cggcgactcc aactcagcgc atagtccttc agctccaccg attccatctc cggcgctact
     4921 gtgaatggcg tcttggactc gatcatcacc gccacttcgc tcgtctgagt cttccccttg
     4981 ctggcttgca ccgcttgcgg ctgcgggccg tggtggatcc cctgtgggtg caatgtcagc
     5041 attcctggct gtatcccggc gcggctgaag aactctccct gatgatagaa aagcacttca
     5101 tcatagtccg tattgcgatg gtaaaacgga acgcggagtg actcgggatc gctttccaat
     5161 ggtctcggaa cgaaggttga aatgaggcac ccaccggctc tgaatgtcac atggacgctc
     5221 ggcggcaaat ggtagcgggc actcgtgacc ggccggaaat cacgcacgtt caacttggcg
     5281 gcccacaagt ctcctttcca ccccatcacg tccattggat agaaggggta aatcacccgc
     5341 gtcgagtcgc cttgtcgctt gatgcggacc tcccattctt ggccctgcgt ctccggaggc
     5401 tccacggtgc cgagctcagg cgcgacaaga acccccttgt caaaaagcgc gtgctggccg
     5461 agcagcccgc gatccggcac cgtgataggt tcgggggttt cgatgacaag aaaaagggaa
     5521 ggaccggcat cgatgtgagt tcgataggtt gtgcccttgg gaatcaccac ataatcgccc
     5581 ggttcgtatg acagcgttcc atagtccgtt tcgaatctcc ccctgccttg atggacgaag
     5641 tacacttcgt ctccgtccgc atcacgaacg aaatagcgca tcgtctccgc acgatacgaa
     5701 acatgaaggc gcacatcctg gctttgcatc acaagagtct gttggtcagt tgcatgtgat
     5761 gggctcgtcg tggagagctg ggtacaagta aaggcccgcg gcttcagcgg cccgtcaatc
     5821 ttcacccacc ccgtcggtgg atgtgttcga tacaaatgcg agaccgggcc ggcaaaccct
     5881 tctcgcccat gctcctcctc aaagagccct tcaggaatcc cgacgtgagc ctgattggga
     5941 actttgcctt ttttgttgag atacatggcc ggcctcccca gagtcagact ccgttcatga
     6001 cgcctgcttc atcgtcttct tcgtgatggg aaccgtgggt gattggtagt caatgatcgt
     6061 ccgttcaacg ccggaggcct gatctctttc aatgtccttg tagagcgctt caaccgtact
     6121 ccggacaaaa gtcttcgcct tttccgttcc gtgcgctcgc tgggtcaact cgatgaacaa
     6181 cccgccgctt tggaaaagtg gatgagtaaa ccgctgcttc agcggtccaa acccgtctcg
     6241 cccttctttc aatggcccgc tgaatttcac cccgcgcgtt tcccactcac gacacacggc
     6301 ctgaatatca tcaacctcga gagcgaagtg ctgcacaccc tgcccgtact tctcaataaa
     6361 ctcggtaatt tgcgacttca tcgttttatc tcgaccttgc ataagcgcaa ccctggctgc
     6421 gcctcgctgc acgacgaccg tgtccatcga cgacttgtcg ctgcctacgt cgcgagccga
     6481 ccagatcact tcgaaaccga gaatcttggt gaacagaaat tccgccgcat cgagatcctc
     6541 tacgcagagc gtgatgtgat caatcccctt tgtttggtcc atggtaacgc ctcctctccc
     6601 cccggggcgg aattcattct tgcttcatca cactatcatc catcccggtt ggtttcagac
     6661 cttcgagcaa ggacaatata acatatattt acaatcgctc aatattaaaa cattatcata
     6721 actgtaaatt tgtaacagtg cgattatgca taactagtgc caatatactg aataagattc
     6781 gctctatgct ttgttctact gtgaacaata gttgataaca caatataaaa gttatatcat
     6841 ccacgtggag tgcaagatgg tttattcgtc acttccaatt gcccatgcgc ttccttctct
     6901 ataggaggtg gccgatgacg ttctatcaag aaatgagaag cgtcgtactt ggacacgggg
     6961 ccatcaacaa ttcgtatctc gatcgctttc aacggggaaa tttgacggac gcagagttga
     7021 aggaattcgc catcgagttc tatagctttg cccgtttctt ccctcaaatt ctggtgtctc
     7081 aactcgtgaa caccgaggac gagaatgttg ccgatgaact gaccaaggtt ttgtactccg
     7141 agttgggcga tggacagaca aaacatcgcc acgagctctt atatcgagac ttcttgcgat
     7201 ccatcggcat cgatgtgcat gaggccatgt ctcgcccaat gctgccctcc acccgcgcct
     7261 acatcgaggg gatggaacgt ttgtacagtg acggcaatca cgccaaagcc ttaggcgcat
     7321 cctttggcct tgaaaacatg gctatcacca tgtgggattg cctgatcccc ggtcttacct
     7381 ctctgaaatc caccaactac tctcgcatgg acatgactta tttcaccttt catcgagaac
     7441 tcgaatccac acatgaggaa gcgatggaac atgcggtggc tgccctgcag ggtacgacca
     7501 gcgatggcat gtccgatcag gaaaaagcag acttccggtg cggcatgaat gccgtcctcg
     7561 ggtatctcga gggtttttgg atggggttag agcgtagcgg gtctcccgac cctgaaccac
     7621 agaggagcca ctcgcacgca gcatgatgtc ttagcgcgaa cacgttgcga ccaactcttg
     7681 ggaggagcag tgcatgtccg aaatcgcaaa gtatcttgag gccatcaagg aacggttcga
     7741 tctcagcagc gattacgcgt tggcagagaa actgggcatc gcccagccgg aagccaatct
     7801 catgcgacgc ggtctcaaga tcccgaaacc cgagctctgc atcaagattg ccaaactcct
     7861 caacaaaaat ccggtagaac ttctgttgat tgcgcagaag gacaaggctc cctcctcagc
     7921 caaggaatac tggactttgg cgctgacagc cgtcgacgtc atgctccacg ttccgaagac
     7981 tcccaagtac attccacgta aggtcgaagc gatcggacgg gaacttcgcc agcttgaaac
     8041 gcagacgcta acctatcaag gcgcagaggc gaacgccgag gcggtccggc ttgttcagac
     8101 ggccgaaaat tccatcgacg cgatcatgga gcgctggaac atctggaaaa aaggcgaagc
     8161 cctgtatccg agctatctcc tggccaacca ggaagcggcc aaccgcggag tcgttattcg
     8221 ccgcctgttg gtcttgacgc gagagcaatt gcgacaggag tcggtcgtgg ctgatgccat
     8281 ccaggtcatg gacgaccagc agcgggctgg catcaaaatt ttcttcgcgt tccgcgaaga
     8341 gcttgaacga gcacccgcct tccaacggtt cgaagaagat tatgaagccc aaggagccgc
     8401 gaaggacctc aacgcagcca tttttgaccg agagattctt attttttctc aatcctacgg
     8461 ccaggtccag ctgggcgtgg tcggcgtgcc gaccgctcta acgatgatca accagttgga
     8521 gatcacatgg aagccggatc ttcttcgcga tttggatcct gctcctctct ttgacatgac
     8581 acggtatgtc tttgagtacg gaggcccgca ggcctttaac atggaattgg cacggttcaa
     8641 gaggtcggtt gcatgaaatt tccttcagag ccgttcaaaa tcaaagtggt ggaaccgatc
     8701 cgtcgcacga cgcgcgagga gcgggatcga ctcctgtgcg gggccggcta caacctgttc
     8761 cagctgccgg ccgagagtgt gtacgtggac ctcttgaccg acagcggcac atcggccatg
     8821 agcgatcacc agtgggcggg cttgatgctc ggagacgagt cctatgccgg cagcaagaac
     8881 tactaccatt tcgaaaagac ggtgcgatcc atctttggtt acaagcatgt gatccccacg
     8941 catcaggggc gcatggcgga gaatcttctg ttctccacgg tggtgaagcc aggcatgtgc
     9001 gttcccaaca atattcactt cgacaccacg cgagcgaaca tagagcatct gggagcccag
     9061 gcgttggatc tggtggtcaa agaggcctat gacccgagct gcgacttgcc gttcaaggga
     9121 aacatggatc ttgtccggtt ggaggacacg atcaatcgtg tcgggcggag ctgcattccg
     9181 ctggtcatgc tgacgatcac caacaacagc ggcggagggc aaccggtgtc tctgggcaac
     9241 atccggcaga cccgggtgct gctgagccgc taccgcattc ctctcttctt cgatgcctgc
     9301 cgtttcgccg agaactgctt tttcatcaag gaacgcgagc ctgggtacgc gggcaagtcg
     9361 gtttttgaca tcgcacaaga gctattcagc tacggggacg gctgtaccat gtcggccaaa
     9421 aaggatggcc tggtgaacat cgggggattt ctgagcctaa acgatgatca gtgggcgcaa
     9481 gacatcacca acatgctcat cctggtcgaa ggctttccta cctatggtgg gttggcaggc
     9541 cgtgatctgg aagcgatggc cagaggactc cgcgaagtgt tggatgaaga ctatctgagc
     9601 ttccgggtcg gacaggtccg ctatttgggc gagctattgg accaagcagg agtgcctatt
     9661 ctcaaaccca tagggggaca tgcagtctat ctcaacgcga aggagttctt gccacatatt
     9721 ccgcaagatc actttcccgc gcaatcgttg gccatctctc tctatcggga ctacggcatc
     9781 cggggagtcg aaatcggtac agtgatgttc ggcaagaaag atgcggcaac cggtcgaacg
     9841 atccatccgg cacttgagat ggtgcggctg gccattccgc gccgagtcta tacgaatatg
     9901 cagatcacct atgtcgcaga atcgatcatc gagctgtacc gccgccgcga ggggattcaa
     9961 ggtctcacct tgacctatga ggcgccgatg ctgcgccact tcacggcgcg atttgaggag
    10021 attcaacacc ggcctctcct gcagcaaacg gcgtcatgat ggagaaccta tggcgaccct
    10081 cttcaaaggt cttgaacaaa tcgaagaagc ccgggagcat gtaaccggcc acggttttat
    10141 ggcaggtgtc tatgtgggaa ggcctgactt ttccctgctg ctccagccgg aagaatcacc
    10201 ggaacagaag gccgcctggg agcgatatca ccccctgttg gagaccttcc tcagaacgca
    10261 cgtcgatcct gatgaaatcg aacggacggc gaagattccc gattccgtgc tgaaggggtt
    10321 gttcgcactc ggcgcgttcg gcatgaaaat ccccacggag tatgggggac tgggcttctc
    10381 ctatacgaac tatgggcggg cgctgatgct catggcaagt tggagcaata tcctggccct
    10441 gaccgttgcc gtgccgcagt ccatcggcat cgccatgccg atacttctgt tcggtagcga
    10501 agaacagaaa cacaagtact tgccgctcgt cgcccgcgaa gccatctcgg ccttcgcgct
    10561 caccgaatcg aataccggat cggacgccgc taatatcagg acggaagcgg tgctggattc
    10621 cagcggcacg gcgttcgtcg tgaacggcga aaagctctgg tgtacaaacg gttccatcgc
    10681 ccgctatgtc acattgatcg cacgggtgcc ggcgaaacgg ctcatacaca acgggcgacc
    10741 cttgtgggtc ccggtaccgg aaggcaaagg cgcagacgag caggttcaca ccgcctttgt
    10801 tctcgatatg gaaacgccgg gcgtcctggt gcggcagcga tgtcagttcg aaggttgccg
    10861 gggcattgag aacgcccaca tgaccttcgc cggtgcccgc atcccggcgg caaacgtgat
    10921 cggtgaggtc gggcgaggcc ttaagtatgc tctgaccatt ctcaatgtcg gccgcgcgat
    10981 cagtgtccca gccatctgtc tcggtatggc caaacaggcg tggcagccga cgctcgatcg
    11041 ggctaaccag cgactgacct ttcagaagcc tctcagcgag cgacagacgc agcgcatgag
    11101 gctgggtcat atggctgccg gtctctttgc catggaagca ctcgcttcga ccgtatggcg
    11161 catggccgac caacacacct acgatgtccg catcgaagcg gctatcgcca agatattctg
    11221 ttcggaacag acaattcagt tcttgaaaga cgcccagatc attttcggcg gcatgggtta
    11281 cgaaacagca gagtccaagc ggtttcgcgg agaaccgacg ttcagcgtcg agcaactggt
    11341 gcgcgatgcc gagatgtatc gaatcggcga aggagctacg gacgtcttgc gaccgttcgt
    11401 cgcgcgcgag ggcctcaaca cgcatcttga gcgggctcgc aactatgtcg acgagcgggt
    11461 ggccggtctt cgccggctca cagaattgcg acatctcttg agattctata tcccctggta
    11521 cgcgcggcag tgggtgcgca ggccattacc ggctcgatct gagttccaac atccccaggt
    11581 ccggcctaaa ttggctttcg tcgaacgagc gagtcgtcgg ctcgcccggg cgattttcta
    11641 tgccatgctg ttccatcggc aagcgcttca agatgaccaa ggccgccaaa accggatcga
    11701 gatcatcggt gaagaccttc tcgtcattgc ggcgataact ctttatgccg aacggcagga
    11761 aaagatcgcc ggacatccgg agatttggga tcttgcagag gaggtcttcc gtgagggcaa
    11821 gaaggacatc gatcaaaaca ttcgagaact tatctgcaat cgcgatgaac tggtgacagc
    11881 tgttggacag agggcgctca gtggcacgta cccttcactc tcggacggaa tcatccggcg
    11941 cggccttcaa gattacctgt tcagacagga gagtgaacga aaagcaatct gagccggtac
    12001 tccaccatct atgggcatac cttctagtat cccgcttctt caactttaag aagacgccgc
    12061 ccgtgccaat ccaaaaccgt ccctgagcag taacccttat cgatacgccg atgaggacaa
    12121 tgtcatgcat gccaggagca accatacgat catacacacg ggcacttgag gtcatcactg
    12181 tggcggccat ggagccacgt caaaatgaga tgagcgcatg cgtgcttggt tgatggattc
    12241 ttatgatggc atcgaaaggc tgcgcctcgg cgaagtgccg gatcctcaac cgggtccggg
    12301 ccaggtgctg ctgaggatga ggtttgccgc tttgaacccc gcggatgcgt ttttagcaca
    12361 ggggatgtac ccgaccaagc cgccgttgcc acacattctc gggcgtgatg gggtgggtga
    12421 tgtggtggca atgggggccg gcgtgaacaa tgtgcgcatg ggtgaggccg tgggaatcct
    12481 acgctgcgat atcggggtga acgtatgggg aactctggcc gagaaagtca tggttcccgc
    12541 tgaaaatgtg attcgaatcc cttccggctg gtccctcgaa gagatggctg gcgctccatt
    12601 ggtctttttg accgcctggc aggccttgac gcagtggaac gatcctccgg cacctccgca
    12661 cacggggtct gtcctcctcg tgaccggcgc gtcgggcggc gtcggcgtgg cgtcagtgct
    12721 acttgggcaa tcaatgcagc tcaccgtcgt ggccttgtca cgcagtgcgg agaagcgcgt
    12781 caggttgaag acactcgggg ctgatttcgt ttttgatcca gcggacagaa acctcatcaa
    12841 agccgtgatg gcggcgatca gtccaaagaa agtcgacctg gctgtcgaca gcgtgggagg
    12901 tgcgctactc cctcaagtgg tggcaatgct tggctatggc ggccggatca gcgtggttgg
    12961 caggagcgga ggcatcgtgc cggagttcaa cacggcgacc ctgctcttcc gccgcattcg
    13021 aatgggcggg gtttcggttg gagacgaaac ggctcaggca gcacacatgg tatggaagga
    13081 gatcgtcgct cgactggatt ccatggggca gcgaccacaa gtggacgcca cagtttcgtt
    13141 cgaagatgtc aagaaagggt tcgcgcgatt ggcccagggg cccatgggca aggtgctggt
    13201 gcaagtggct gcctaacgcg acctgacctc gtcctgtcgc taacacggtg cggatggttt
    13261 agggaggatt ttccgtgctc catgtaaaat cgacttggtt ttcacaaaac agattgagca
    13321 atgtcttgaa cagcttcagc ggccgcggtg gcgtgcctcc atctctatgg cgctgcgact
    13381 agataagaga caggcctctc cctttgagaa gactgaactg tctctgaccg gttcacggac
    13441 tccccaaaat ctcgcccgat tcggtcaacc cgtgtgttca gaacgatgat tccacttgcg
    13501 atctttcatg gtagtcgaaa gaaattctcg ccaggattga actgaccagt ctgcaggcac
    13561 gatcagacgt cacctgacaa cgggattatg agccggcact tcagcgttgt tcatgaagct
    13621 gacaaacggg ccgaagaaca atcggcggat caacgagagg cgagcgagaa cgaggtaacg
    13681 aaagctccag aattattgtt ggcttccata atttgttgta cagaaccagc gcattacggc
    13741 cgcgggtcca gttcgatcaa gcctttcctg atagccagga tcgcggcctg tgttcggtcg
    13801 tagacttgga gtttgtggaa gatgttacgg acgtggttct tcacggtctt ttcactgaga
    13861 tccaagctgt tggcaatctc tttattcgtt ttcccgtccg cgaccaagcg caggacggtg
    13921 atctcccgct cggtgaggtc gtgttcaacc caggccggtt ttttcccctt cttctgggcc
    13981 atcaaagaaa actctgcgag gatcttgctg gccacggatg gatggatcag cgattcgcct
    14041 cgatagatcg cacgaatggc ggcgacgatt tgcgaagatt ccgagtcctt gagcaggtag
    14101 ccggtggctc cggcccgcac gagatcgaag atgtattgct gttcttcata catggtcagg
    14161 gcgacgatgc cgatgtgcgg gaactcgcgt ttgatctgcc ttgtggcttc tactccgccc
    14221 atgcggggca tgctcacgtc catcagaatc acgtcgggca ggagcgtcct ggttttttcg
    14281 accgcttcca ttccgtcctg ggcttcgccg accacgttga tatcgtcctt tgttttgagg
    14341 atggcggcga gcccctcgcg caccacacga tggtcgtccg cgatgagaac cttaatcttt
    14401 tccattgtcc gagttctcct tcttgcctaa cggcacctcg acgacgatcg tggtgccgcg
    14461 acctttcttc gattcgattt tcgcctcccc gcccacgagt ttggctcgct ccaagatccc
    14521 gcgaatgccg aaatggtccc acttctcagg atcctgcatc acgtgctcca tgtcgaatcc
    14581 caccccattg tctttgatcg taacggtgag gcgatcgaac tggatctcca gccgcacgga
    14641 gacgcgatcc gctttggcat gttggaccac gttactgagc gcctcttgca cgatgcgaaa
    14701 cagaaagatc ttcgtacggg ggaacaggat ttgttcgtcc cccgacacct cgaactcggt
    14761 cttgatgtga tactgggtct ggtaggactt gaggtaattc gtgagcccgg gaatcaattc
    14821 cattttgtca tactggaggg gacggaggtt gaaaatgact tggcgggcct cttggatcgc
    14881 caatttcagt tgctccttgc tctcgcgcaa cgttgccaag ctggccttgg gattcttgcg
    14941 gatcagttgt tggcacaact cgagcttgaa gttgactccg gcgaggcttt gaaccagccc
    15001 gtcgtggatt tcgcaggcga ttctggtgcg ctcctctgtc accgcggccc cggtttcttt
    15061 cacatacagc cgatacaggg attggtactt attgagcgtc ttctcgatct cggctgtcgc
    15121 ccggatcctg gcctcgatca attcccacat aaatttggcg ctggctccgc cgatgatcgt
    15181 gacgcccatt cggtgaaagt cttggagaac ctgccagacc tctgcgttac ccgagacgtc
    15241 gatcaccaga tccaccctct ccaagtcgag caggcgccgg tagtcacgtg tcacggggat
    15301 ctttaaacgt ttagctaaag cgagcccggg ggcgctgggg ttgcactccg cgacgccgac
    15361 gatttgcacc aacgggtcgg ttgcaaaaat ctccatcagc gcggtaccgc cgcgtcccgc
    15421 gccgatgatc gccacatgcg tggcgcccgt ggctccgttc ctgcgcattc gtttcgatgg
    15481 tttggcgtga actgtcggca tttgtgcgcc tagtcccggc gtctccttgt tgtgtcgcga
    15541 acgaactggg aagggagtca gaaagggccg ggaaagaact atcagaccat tgccttcacc
    15601 gctcccggcg ttgttgggcc aagctcttta gctcatccat gaactggtcg atgtccttga
    15661 actctcgata aacggaggcg aatcgaacgt aggcgacttg atcgagttga tgcagttctt
    15721 tcatcagttc ttccccgatc acgcggcttt caatctccgt ctcccccatc tcctgaatgc
    15781 gcttttcgat cctgtcggtc acggcctcga tggtggcagt gctgatgggg cgcttttcac
    15841 aagccttctt cagacctgaa agaattttcg accgatcgaa tgtctcccgg cgtccgtcct
    15901 tcttgacgac gacggggaga atttcctcaa cacgctcgta ggtcgtgtag cggcgtttgc
    15961 aacccaggca ctcgcggcgg cgccggatga cttcaccctc cttcgccatg cgcgagtcga
    16021 cgaccttgtc ttcgagctca tcgcagaagg gacatttcac cggcggtgac cctgtcctgt
    16081 catgtgtggt tagtagggat gaaagatcgg gaaccggttg cagagcgcct tggctttggc
    16141 gcggacttct tcgagtatcg ccgggtcctg ccggtgttgc agcacacgat cgaccagggc
    16201 gacgatttcc ttcatctccg cttccttcat tccgcgtgtc gagacgatcg gtgtgcccag
    16261 ccggatgccg ctggcaatgg cgggcggctt ttcgtcgtag gggacggcgt tcttgttcac
    16321 aatgatgccg gcggcatcca gcgcggcatc cgcttccttg ccggtcaagc ctttgttcgt
    16381 gagattgacc aacatgaggt gcgtatcggt gccccctgat acgatcttgt atcctcggtc
    16441 gagaagcccc tgggccagga ccttggcatt ggccacgacc tgctgctgat accgtttgaa
    16501 tgagggcgaa agcgcttctt tgaatgcgac ggccttcgcc gcaatcacgt gcatcaacgg
    16561 accgccttgc attcctggga agacgaattt atcgacggcc ttggcgtgct cggccttaca
    16621 cattgtgaca ccgccgcgtg gccctcggag tgttttatgg gtggtggtcg tgacgaagtc
    16681 tgcataagga acaggattcg gatgcaaacc ggctgcgatc aagcccgcga tgtgggcgat
    16741 gtcgaccatg aggtaggcgc cgaccgattt ggcgatctct tggaacttcg ggaaatcgag
    16801 ggttctcgca taggcgctgg cgccgacgac gatcatgcgc ggacggcatt cttcggccaa
    16861 tttctgcacc gcctcataat tgatggtttc cgtctggcga tcgacaccgt aggaaaacgc
    16921 ccggaagatc atcccggaga aattcacctt gctgccatgg gtcaagtgac caccctgggc
    16981 caggtccata ccgagaatgg tgtcgccagg cttgaggacg gacagatagg ccgccatatt
    17041 ggcttgtgat ccagaatggg gctggacatt cacatggtcg gcgccgaaga tctctttgca
    17101 ccgctggatg gccaggtctt cgacagtgtc gacgtgctgg catccgccgt agtaacgctt
    17161 ccccggatag ccttctgcgt acttgttcgt catcagggag ccctgtgccg ccaagaccgc
    17221 cgggctggca aaattttccg aggcgatcag caggagcttg ttgcgttgcc gttcttcttc
    17281 ggcatcgatg gcagcataga cctccggatc ggtcgcc
//
LOCUS       DATIFR010000008         8716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2110127, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000008 DATIFR010000000
VERSION     DATIFR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110127"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VLD60_00275"
     CDS             complement(<1..714)
                     /locus_tag="VLD60_00275"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HSB43410.1"
                     /translation="MITQATPSNASTALQFASALSRKTDTEAAVRDLADAIRTQIGQA
                     PLDVGFVFFSAHHAEKSRLIATMIREELRIEVCLGCSGEGVIAGAEELETAPALTLWV
                     ASLPLVRVTPLRLSFSQVQDQVNMTGWPEPGADASTFILLADPFTTPVHDVLSVMADR
                     YPNGQAVGGLAGGGHGEGENRLILNEEAFEGGLVGVQLSGPLTVRTVISQGCRPIGER
                     FMVTRSEHNLIYELSGKPAL"
     gene            complement(733..960)
                     /locus_tag="VLD60_00280"
     CDS             complement(733..960)
                     /locus_tag="VLD60_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43411.1"
                     /translation="MNEYQIGGGLRLLTAVEKTEAFGDFLKTRMVRALETEDPTELHY
                     LLAQLDDYYSYMWRYYKKLAKDRAERMNPGV"
     gene            complement(1038..2240)
                     /locus_tag="VLD60_00285"
     CDS             complement(1038..2240)
                     /locus_tag="VLD60_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSB43412.1"
                     /translation="MKYNRITISSLILVTLSCTFTPHLPARAEGAPEPRLRARELGIR
                     IGSYPTGPLNAITDVTGVKVGHTTVIKGEGQLKPGEGPARTGVTVIIPREDVWHKKVA
                     AGSFVLNGTGEMTGLAWVAESGFLEYPIALTNTLNVPRVANGVISWMIKQYPEIGITD
                     DTLTPVVAECDDSRLNDIQGRHVTETDVAMALDSASGGPVAEGTIGAGTGMVSYGFKG
                     GIGSASRRLPDKEGGYTVGVLVNANHGRRPELMVSGVSVGRLYDAPAPAAQGNQPGLS
                     EGSIIIIVATDAPLDSRQLTRLAKRAALGLARTGSTARHGSGDFILAFSTANVIPHYP
                     EESTYSLLHMADTHLNPLITATVEATEEAILNALTMATTVTGRDHHRIEAISIARLRT
                     LLERRDIP"
     gene            complement(2230..3627)
                     /locus_tag="VLD60_00290"
     CDS             complement(2230..3627)
                     /locus_tag="VLD60_00290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB43413.1"
                     /translation="MRLSFHGAARSVTGSRHMLEVPGFRLLLDCGLFQGRREEALRQN
                     RDLGFDPKSLGAVILSHAHIDHSGALPVLPRHGFSGKIHMTRATADLAEIMLEDSAHV
                     QESDCSYVNKQERRRGKACVRPFYDGDDVRKIVRRFEGARYGDTLKISPRVTATFHDA
                     GHILGSAAIRVKYTARGNTTTVLFSGDLGRSNMPILRDPEPPPPCDVLILESTYGDRL
                     HEQAGEEMTKKAQDLIAHARTHKSKIIIPAFAVGRTQELVMRIKELVKEGRIDPIPIY
                     IDSPLAGKATEVFRRHPECYDEETFKTFSADGDPFASRYIHFVSSLEDSKRLNTMKGP
                     CVIISSSGMCEGGRVVHHLKHAIEDEANVIVFVGFQAEHTLGRKLVEGWDVVPIFGVP
                     TKRRAQIVKFNGFSAHADRGDLLAYLNAIDPLPDRVFIVHGEEKQALSLATAIQTAHP
                     PIEVTVPHLGSIHEI"
     gene            complement(3624..4478)
                     /locus_tag="VLD60_00295"
     CDS             complement(3624..4478)
                     /locus_tag="VLD60_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSB43414.1"
                     /translation="MSPSPSDHPSSPDSVKPSSSYIPADRDAEFLQRDELRSVRIGLE
                     LLKPELIQRELGIQSTIVVFGSARLQEPSVARETLRLAEEAAARTPEDAACRKKVATA
                     RRQLDLSKYYEIAREFSQLVSSTRQVDGRCDHVIVTGGGPGIMEAANRGAADVNAKSI
                     GLNITLPHEQHPNPYITPELSFQFRYFAIRKMHFLIRAKALVAFPGGFGTLDELFETL
                     TLLQSGKTQDVTVVLVGRAFWDRLINWQWLVEQGLISQDDLALFHYAETAQEAWNLIA
                     RHNGVPSS"
     gene            complement(4554..4862)
                     /locus_tag="VLD60_00300"
     CDS             complement(4554..4862)
                     /locus_tag="VLD60_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43415.1"
                     /translation="MVNGKTSLSEVIEKLGAPNQMLSSSGGVVARYQFTDGKYFRVDF
                     GWGLRFIIPFYSPELVLGDGGMSVDVFQLTYNDQWVVQDHGFAFHSQSSAFRLWPFKD
                     "
     gene            complement(5019..5438)
                     /gene="bamE"
                     /locus_tag="VLD60_00305"
     CDS             complement(5019..5438)
                     /gene="bamE"
                     /locus_tag="VLD60_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HSB43416.1"
                     /translation="MNSISCDHGAILHGKYRGPALTTWLILMLLVGSGCAFVRGSYGE
                     DVNQGDVSTIKKGVSTRAEVAAILGAPDRIITEDKGHDIFHYYHYDVKSGYVLIFSRI
                     NIKSDDIFVIFNSAGIVEDVIAGKKKPPLEFQFWPFD"
     gene            complement(5435..5920)
                     /locus_tag="VLD60_00310"
     CDS             complement(5435..5920)
                     /locus_tag="VLD60_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB43417.1"
                     /translation="MRLALPEDIDSIVAFSAAMALETEGRQLDQARLREGTRSLFTSP
                     DRGFFMVAELSQVDGPKLVGQLMITYEWSDWRNAVFWWIQSVYVDARWRRRGVFKRMH
                     EAVLQEARARNGVCGVRLYVDKDNPIAHISYERLGLVRSAYQIYESDFVLPPKRPKES
                     R"
     gene            complement(5948..6562)
                     /locus_tag="VLD60_00315"
     CDS             complement(5948..6562)
                     /locus_tag="VLD60_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSB43418.1"
                     /translation="MKTLWRHSLAGLLILVPAWATFLILSAMLTTVNRLISDLLSQIG
                     ISATPLFGLILFILLILSAGAIATHFLGKQLIASAERWIEQIPLVRSIYLTIKGMTDL
                     FQFRSRFGRSTVVAFPFPRNGLWALGFVMGPAPQAIQVEPSSTLMMIFVPTAIHPFTG
                     YLAFVPEQEIRPLNLLPEDAMKMEFSAGFYTPGKGWLGAPKATV"
     gene            6712..7002
                     /locus_tag="VLD60_00320"
     CDS             6712..7002
                     /locus_tag="VLD60_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSB43419.1"
                     /translation="MADHAGTRRPVQIDKDLLAILCCPETKLEVSLAEEVLVAKLNDA
                     VSRGALKNKAQKPVTELLDGGLIRADRKILYPIRENIPVMLIDEGIPLDGIA"
     gene            complement(7459..7689)
                     /locus_tag="VLD60_00325"
     CDS             complement(7459..7689)
                     /locus_tag="VLD60_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43420.1"
                     /translation="MMREWIIFALCLGVGGHIALGVVLHAPELWPWSTAGFYGLLSGL
                     AVYAGAQGIRGLWKLLKSSPQASTKGPSGSSW"
     gene            complement(7766..8137)
                     /locus_tag="VLD60_00330"
     CDS             complement(7766..8137)
                     /locus_tag="VLD60_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43421.1"
                     /translation="MSSTLQRAVTSFYNLVYEAQTFATTMARIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDTNLTPSSLQRVLTEVKSDLDHELSVQRREKGRRADGREEALNFV
                     ADYLSSLITATEIESAKTPAA"
     gene            complement(8224..8553)
                     /locus_tag="VLD60_00335"
     CDS             complement(8224..8553)
                     /locus_tag="VLD60_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43422.1"
                     /translation="MTFRQDQPIPFAAQAIPFDEFLAAEKLPEGYLTSEYVAQQFVER
                     LVHYILSVPAGSYTMAQLSHLLEQLDPRTQVFFFKRLKETSPDSLKDFAPLYYGFMNE
                     FHSLLFT"
BASE COUNT         1932 a         2333 c         2569 g         1882 t
ORIGIN      
        1 gagcgccggt tttccgctca gttcatagat gagattgtgc tcggacctgg tcaccatgaa
       61 tcgctcgccg atgggacggc agccctgcga aatcaccgtc cgaacggtga ggggcccgga
      121 gagctggaca ccgacgagtc caccttcgaa ggcttcttcg ttcaggatga ggcgattttc
      181 cccttcgcca tgtccaccgc ccgcgagacc gccaacggct tgcccgttgg ggtaccgatc
      241 ggccatcaca gatagcacgt cgtgaaccgg agtagtgaac ggatcggcca gcaggatgaa
      301 ggtggatgca tctgcgccag gctcaggcca gccggtcatg ttcacctggt cttgcacctg
      361 agagaatgac aagcgcagcg gcgtcacgcg aaccagtggt agagaggcga cccagagcgt
      421 cagcgccggg gcggtttcca attcttccgc gccggcaatg actccttcgc cggaacaacc
      481 caggcagacc tcaatgcgaa gttcctcccg aatcatcgtg gcgatcaggc gcgatttctc
      541 ggcatgatga gcggagaaaa agacgaaccc cacatcgagc ggcgcttgac cgatctgcgt
      601 tcgaatggca tcggccaggt ctcgaacggc tgcctcggta tccgtcttgc gggagagagc
      661 agaggcgaat tgaagggctg tggaagcgtt gctgggagtg gcctgggtaa tcattcgctc
      721 ggccttatgt agttagacgc cgggattcat gcgttcggcg cgatccttcg ccagtttctt
      781 atagtaccgc cacatgtagg aatagtagtc gtctaactga gccagcaggt aatgcagctc
      841 cgtcggatct tccgtttcca atgcgcggac cattctggtt ttgaggaagt cgccgaaggc
      901 ttcggtcttt tccacggcag tcaagaggcg taacccaccg ccgatttgat actcgttcat
      961 ctgattcctc cacactcgaa gtgtgcggcg tgaggatagc gaggggacgg atcaaaatgc
     1021 aagcaggctg tctgctccta gggtatgtct cggcgttcga ggagggtacg caatcgcgcg
     1081 atcgaaatgg cttcgatgcg gtgatggtcc cgtccggtca ctgtcgtggc catggtcagg
     1141 gcgttgagga ttgcttcctc agtggcctcg acggtcgcgg tgatgagggg attcaggtgg
     1201 gtatcggcca tgtgcaggag gctatacgtg ctttcttccg gatagtgggg aatgacattg
     1261 gcggtggaaa aagccaggat gaaatcaccg ctgccatggc gtgccgttga gccggttcgc
     1321 gcgagtccca gggctgcgcg tttggccagc ctcgtcagct gccgactgtc gagcggcgcg
     1381 tcggtagcga cgataataat aatggaacct tcactgaggc ctggctggtt tccttgtgcc
     1441 gccggggcgg gggcatcgta aagtcgaccg actgaaaccc ctgagaccat cagttccggc
     1501 cgccggccat gattagcatt gacgagcacg ccaacggtgt agcctccttc tttgtcagga
     1561 agccggcgcg aggcgctgcc aatgcctccc ttgaagccat acgacaccat gccggttccc
     1621 gctccgatgg tcccttccgc gacaggaccg ccggaggcgc tatcgagagc cattgccaca
     1681 tctgtctcag tcacatgccg accctgaatg tcgttcaagc ggctatcgtc gcattccgcc
     1741 acgaccggtg tcagcgtatc gtccgtgatt ccaatttctg ggtactgctt gatcatccag
     1801 ctgatcaccc cgttcgccac gcgcggcacg ttcagcgtat tcgtgagggc aatggggtat
     1861 tcaagaaatc cggattccgc gacccaggcc agcccggtca tctctcctgt gccattgagc
     1921 acgaaggatc cggctgcgac tttcttatgc cacacatcct cgcggggaat gataaccgtg
     1981 acgccggttc tagcagggcc ttcgcccggc ttcagctgtc cttcgccttt gatcaccgtg
     2041 gtatgcccga ccttgactcc ggtcacgtcg gtaatggcat tgagcggtcc agtcggatag
     2101 gagccgatgc gaatgcctag ctcgcgcgca cgcagtctcg gttccggcgc tccttccgcc
     2161 cgggcaggaa gatgtggagt gaacgtgcag gagagcgtca ccagaatcag ggaactgatt
     2221 gtgatccggt tatatttcat gaatcgatcc cagatgggga accgtcacct ctatgggggg
     2281 atgtgccgtc tgtatggcgg tggcgagcga cagggcttgc ttttcctccc cgtgtacgat
     2341 gaatacccta tcgggcaagg gatcaatcgc gttgaggtag gcgaggagat cgccgcggtc
     2401 cgcatgtgcc gagaaaccat tgaatttgac gatttgggcg cggcgtttag tcggaacccc
     2461 aaagatgggt acaacgtccc atccttcaac caattttctt cccagcgtat gttcggcctg
     2521 aaagccgaca aacacgataa cgttggcttc gtcttcgatg gcatgtttga gatggtgcac
     2581 gacgcgcccg ccttcgcaca tgcctgagga tgagatgatg acgcaaggtc ctttcatggt
     2641 attgaggcgc ttgctgtctt ccagggagga cacaaaatgg atgtatcgtg acgcgaaggg
     2701 atcaccgtcg gcggagaagg ttttgaaggt ttcttcgtca taacattcgg gatgccgccg
     2761 gaacacttcc gtggctttcc cggccagtgg tgaatcgatg taaatgggaa tgggatcgat
     2821 gcgaccctct ttgaccagtt ccttgattcg catgaccagt tcctgagtcc gacccacagc
     2881 gaaggccggg atgatgattt tactcttgtg agttctcgca tgggcgatca ggtcctgcgc
     2941 tttctttgtc atctcctcgc cggcctgctc gtgcagccgg tctccatagg tggattcgag
     3001 gatcaggacg tcgcagggcg gcggcggctc cggatcgcgt aaaatcggca tattggagcg
     3061 gcccagatcg ccgctgaaga ggacggtcgt ggtattgccg cgcgccgtgt actttactcg
     3121 gattgcggca gagcccaaga tgtgaccggc atcgtgaaag gtagccgtca cgcgcggcga
     3181 gatcttgagg gtgtcgccat agcgggcacc ttcgaatcga cgcacgatct tccgcacgtc
     3241 atcgccgtcg taaaatggac gtacgcaggc ctttccgcgt cggcgctctt gcttgttcac
     3301 gtaactgcaa tcgctctcct gtacgtgagc cgaatcttcg agcataattt ccgccaggtc
     3361 tgccgtcgcg cgagtcatgt ggatttttcc tgagaatccg tgccggggca agaccggtag
     3421 ggcgcccgag tggtcgatat gcgcatggga gagtatgacg gcgcccagcg atttcgggtc
     3481 gaagccgaga tcccggtttt gccgaagtgc ttcttcgcgg cgtccttgaa acaatccgca
     3541 atcgagcagc agcctgaagc ccggaacctc cagcatgtgg cgacttcccg ttaccgaacg
     3601 cgcagcccca tgaaacgaga gtctcatgaa gagggcactc cattgtggcg cgcgatcagg
     3661 ttccaggctt cttgggccgt ttcggcataa tgaaacaggg cgagatcatc ttgagagatc
     3721 agtccctgct ccacgagcca ctgccagttg atcagtcgat cccaaaacgc ccggccaacc
     3781 aggacgaccg tcacgtcctg agtcttgccg ctctggagca gcgtcagcgt ctcgaagagc
     3841 tcatcgaggg tgccaaaccc tccgggaaag gccaccaacg ccttggcgcg gatgagaaag
     3901 tgcattttcc ggatggcgaa atagcgaaac tgaaaactga gttctggtgt gatgtacgga
     3961 ttggggtgct gttcatgggg caacgtgatg ttcagcccga tggacttggc gttcacgtcc
     4021 gcggctccgc gattggcggc ttccataatt cctggtcctc cgccggtgac aataacgtgg
     4081 tcgcagcggc catcgacttg gcgggtcgac gacaccagtt gactgaactc gcgagcgatc
     4141 tcgtagtact tggatagatc aagttgccgc cgtgcagtcg ccaccttctt ccggcaggcg
     4201 gcgtcttcgg gtgttcgtgc cgcagcctct tcagccaacc gtagggtttc tcgagcgacg
     4261 gatggctctt ggagtctggc gctgccaaag accacgatcg tcgactgaat gcccagttcg
     4321 cgttggatta gttccggctt caggagttca agtccgatgc gtacggagcg aagctcatct
     4381 cgctggagaa attcggcatc gcggtccgcg ggaatgtagg aagaggatgg cttcacgctg
     4441 tcaggagatg agggatgatc ggacggtgag ggactcatgg agcacgatta tagacggacg
     4501 cgccgtctgc ggcaattctc gcttcagctt gccgatatgt tcaggtcaaa cggttagtct
     4561 ttgaagggcc agagacgaaa cgccgaggac tgcgaatgaa acgcgaaacc atggtcctga
     4621 acgacccact ggtcattgta ggtaagttgg aagacgtcaa cactcatgcc tccatccccc
     4681 agaacgagct ctggcgaata gaacggaatg atgaaccgga gtccccatcc aaagtcgacg
     4741 cggaagtatt ttccatcggt gaactgatat cgagcgacga ctccaccgct ggatgagagc
     4801 atctgattcg gtgcgccaag tttttcgatg acctctgaca gactcgtttt gccgtttacc
     4861 acaaagctca catcctgggg agaaagcggc tggttgagag tcactcgttg caatgacaca
     4921 gggcctcgaa gacaaccgga aatcgcgatc aaataaagta tggcccagag aaaagtctgc
     4981 aatcttcgta ttgtcatgtg gaaggagggt tggaatgatt agtcaaaagg ccaaaactgg
     5041 aactcgagag gaggcttttt ctttcccgct atgacatctt ccacaatgcc agcactgtta
     5101 aaaatgacaa agatatcgtc acttttaata ttgatgcgag agaagatcag aacatatccc
     5161 gatttcacat cgtaatgata ataatgaaag atgtcgtgtc ccttgtcttc ggtaataatg
     5221 cgatccgggg cacctagaat ggccgcgacc tcggccctgg tgcttactcc tttcttgatc
     5281 gtcgatacgt caccctggtt gacgtcttcg ccatagcttc ctcgcacaaa cgcacagccg
     5341 cttcccacta aaagcataag gatcagccag gtcgtgagtg ccggtccacg atactttcca
     5401 tggagaattg caccgtggtc acaagagatc gaattcatcg tgactccttt ggtcgtttag
     5461 gaggtagaac aaaatcagat tcgtagatct gataggccga ccgaaccagc ccaagtcgct
     5521 catagcttat gtgagcgatg ggattatctt tgtcaacata gagtctcacg ccgcagactc
     5581 catttcgtgc cctggcttct tgcagaacgg cctcatgcat tcgcttgaat acgcctcgac
     5641 ggcgccatct cgcgtcgacg tagacactct gaatccacca gaagaccgcg ttccgccaat
     5701 cactccattc gtacgtgatc attaactgac ccacgagctt gggaccgtct acctgcgata
     5761 gctccgcaac catgaagaag ccccgatcgg gagaagtgaa caacgaccgg gtgccctcgc
     5821 gcaatctagc ctgatcgagc tggcgccctt cggtttccag cgccatggcg gcgctgaaag
     5881 cgacaatcga atcgatgtcc tccgggaggg caagtctcac gatcatctga tgtgtctgaa
     5941 gcatgggcta gactgtggcc ttcggggcgc ccagccagcc tttccctggc gtatagaatc
     6001 ctgcggagaa ttccatcttc attgcatctt ccgggagaag attgagcggc cggatctctt
     6061 gttcggggac gaatgccaga tagccggtaa acggatgaat ggcggttggc acgaaaatca
     6121 tcatgagtgt gctggaaggt tcgacctgga tagcttgggg agccgggccc atcacaaaac
     6181 cgagagccca gagtccgttg cggggaaagg ggaaggcgac aacggtacta cggccgaagc
     6241 gggatcggaa ctgaaacaaa tcggtcatgc ctttaatagt cagatagatg cttctcacga
     6301 gcgggatctg ctcgatccag cgttcggccg acgcgatgag ttgcttgccc aggaaatgcg
     6361 tggcgatagc ccctgccgat agaatgagaa gtatgaaaag gataaggccg aagagaggcg
     6421 ttgcagagat tccgatctgg ctcaaaaggt cactaatgag acgattcacg gtcgtgagca
     6481 tggccgaaag gatcaagaat gtggcccaag caggaaccag aatcaacaga cctgccagag
     6541 aatgtcgcca cagagtcttc acgttgttgg atcctcgagc caggtggccg gcaccgccga
     6601 tagtgtacca gaatcgacag aagattggac ctggggctgc ggtcttgata tccgtcaaca
     6661 ggtgctatat cctgcttcag gaaaccgagc ggacatactc aaggattagt catggcggat
     6721 cacgcaggaa cgaggcgtcc agtgcagatt gacaaggacc tgctggccat tctctgctgc
     6781 cctgaaacca agctcgaagt gagtctggcc gaagaggtgt tagtcgcgaa gctcaatgat
     6841 gccgtgagtc gaggcgctct gaaaaacaaa gcgcagaaac cggtcacgga attgctcgat
     6901 ggtggattga ttagagccga ccgaaagatt ctctacccta tccgcgaaaa cattcccgtc
     6961 atgcttattg atgaaggcat cccacttgac gggattgcct agctgttttt cctctcgctt
     7021 cccccagtca tttgtgcagt gccgcaaggc gtgagcgacc ggctcgcgtg atcacacgga
     7081 gaaggatttc ccgggatccc cgttagggcg aagcggcgtt atgctgtggc taatcggttt
     7141 tggagccggt tgacgttccg cactgctgac agagatattc gtagaggttt cccgtcggca
     7201 atacaagcag caggcgttcg cgggtcgggg ttgcgatccg gcagtgaggg caatagagca
     7261 gggaagcgtt gagagatctg aactgttcgg gcggctcagc ggattgaggc cgtagcgttc
     7321 gacctggccg aggaggtgcg ccaggtggcc atccgggaag attcggtttg cgtggaggtt
     7381 gccgcatagg ggaattgtag ggaagaggga ttgtagaggt caatagtgca aggggtcagc
     7441 gatcaatagg ccggttggct accatgacga accggacgga cctttcgtgc tggcctgcgg
     7501 tgacgattta agcagcttcc agagtccgcg tattccttgg gctcccgcat agacagcaag
     7561 tccgctgaga agcccgtaga agccagccgt gctccagggc cagagttccg gggcatgcaa
     7621 caccacaccg agggcgatgt ggccacccac ccccaagcag agcgcaaaaa tgatccactc
     7681 gcgcatcatg atgggccagg atcacgaatt ataggacgct gagcgtgagg ggtgcgaatt
     7741 cggaatgcac gcactggttc agagattagg ctgccggtgt cttggccgat tcgatctccg
     7801 ttgccgtaat gaggcttgag agatagtctg ccacgaagtt cagtgcctct tcccgaccat
     7861 cggcacggcg ccccttttca cgccgctgaa ccgacaactc atgatcgaga tccgatttga
     7921 cctccgtcag gacccgctgg agcgaagaag gagtcaaatt ggtgtccatg tcctcgagtt
     7981 cctggcatcg atccagaacc caattaaggc cttcgatgcg tccagcataa tgctcctgct
     8041 cagcggtatc gatcctggcc atggttgtgg caaaagtctg cgcttcgtaa acaagattgt
     8101 aaaagctggt cacggcacgc tgtagggtcg aactcataga actccttttc ctttcagggc
     8161 aatattacgt ttcaccaatt atacatgaga atacacaagc aacaaggaaa attttcccaa
     8221 gggttacgta aagagaagag aatggaactc gttcatgaag ccataataga ggggggcgaa
     8281 gtccttaaga ctatcggggc tggtttcttt gaggcgtttg aagaaaaaca cctgtgtgcg
     8341 aggatcaagt tgttccaata aatggctcag ttgggccatc gtatagctgc ctgccgggac
     8401 gctcagaatg taatgcacca atcgttcgac gaattgctgg gcgacatatt cgctggtgag
     8461 atacccctcc gggagttttt ctgctgccaa gaactcatcg aagggaatgg cttgggcggc
     8521 aaagggaatc ggttggtctt gacgaaaggt cacgctgagg gtaccccgtg gctcgcaatg
     8581 gaacttcgca atatcgcaca actctactgg acccccccca acgcgtcaag tggacagaag
     8641 gagcagagcc tctgagccga aagaccggta gaaacaaaag gggcagggtc cctttcggaa
     8701 ccctgcccct ccgtga
//
LOCUS       DATIFR010000009         3459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1344204, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000009 DATIFR010000000
VERSION     DATIFR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344204"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VLD60_00340"
     CDS             complement(<1..713)
                     /locus_tag="VLD60_00340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSB43423.1"
                     /translation="MQIKAEEISAIIKEKIKGFDKQVDVKETGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDVGIKEGDPVKRTGRIAEIPVGEA
                     LAGRVVNAIGQPIDGKGPIKSNLSSRIEVVAPGVITRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQRGLGVFCIYVAIGQKRSTVARVVKTLEEHHAM
                     EFSVVVAATASDPAPMQYLA"
     gene            complement(716..1255)
                     /gene="atpH"
                     /locus_tag="VLD60_00345"
     CDS             complement(716..1255)
                     /gene="atpH"
                     /locus_tag="VLD60_00345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HSB43424.1"
                     /translation="MIKTAVARRYAQALFELLDQSNIEATRGTLNGLSQAMKDSISLR
                     HVVASPAFGVDEKIAVLSELGGRLGCPPVGKAFISQLVKKNRIGFLPEIAEAFAKLVD
                     QSKGTQQITVSSAMALPPAEQDRITTRLRDTLKRDVDVTFETNASYLAGLQIHIGSTV
                     VDSTVRGRLKAMQSLLTKE"
     gene            complement(1382..2464)
                     /gene="mnmA"
                     /locus_tag="VLD60_00350"
     CDS             complement(1382..2464)
                     /gene="mnmA"
                     /locus_tag="VLD60_00350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSB43425.1"
                     /translation="MKRPAVLLGMSGGVDSSVAAALLVRQGYDVRGVTLQVWEHEDET
                     VAASKKWQERGCCKVGIARFVAQQLNIPYEVIDSRQNFRSGVIEDFLHGYAGGTTPNP
                     CVRCNERVKLRGLIDLAQARGIPHVATGHYVRISESHAGHRLYRSADSRKDQSYFLYR
                     LQPDWLPHLIFPVGAMQKAEVWREAESLGLPADELKESQEICFVSHGDYRTFIEAEMP
                     EAKKPGAFIDQAGRQLGEHEGIAFYTPGQRRGLGIAASQRLYVQEVQPTTNTVVLGPE
                     DSLLRSSCDVNDLNLFDRSLLASTTDVDVKVRYATPATPARVEPIGTESLRIHFHVPQ
                     RALSPGQSAVFYRGDQVIGGGIIQPF"
     gene            complement(2551..2955)
                     /locus_tag="VLD60_00355"
     CDS             complement(2551..2955)
                     /locus_tag="VLD60_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSB43426.1"
                     /translation="MWARKEKEQVLHAEPDYENFTLLAKDVDFKGVVNFDGTVRVDGR
                     VEGEIHTSGTLIVGEHAVIKGVVSAGVLMTSGKINGTITAVERIQILKPGVLVGDIRT
                     PSIAIEEGSHFHGMCDMGMQSWEEPRAISIAS"
     gene            complement(3036..>3459)
                     /locus_tag="VLD60_00360"
     CDS             complement(3036..>3459)
                     /locus_tag="VLD60_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSB43427.1"
                     /translation="PAPPTEGSGDVIAFVGKGVEFKGIISYSGTVRIDGYLDGEIHTD
                     GILLIGEEAIIQAKVTAGTIVCKGKITGDIIAKERVKLRAPAVFNGSMKTPMLSMEDG
                     VLFNGGLEMSQGVRELPQGTTLHSVASITSPGIKRING"
BASE COUNT          723 a         1009 c          908 g          819 t
ORIGIN      
        1 gccaggtact gcatcggagc cggatcactg gcggtcgccg ccacgacgac gctgaactcc
       61 atggcatggt gttcttcaag tgtcttcacc acgcgtgcca cggttgagcg cttctgcccg
      121 atcgcgacgt aaatacagaa gacaccgaga cctctttgat tgatgatcgt gtcgacggca
      181 atggcggtct tgccagtctg ccggtcgccg atgatcaact cgcgctggcc acgtccaatc
      241 gggatcatcg cgtcaatcgc cttgataccg gtttgcaatg gttcgcggac cgactgacgc
      301 gtaatgacgc caggcgcgac gacttcaata cgcgaagaaa gattcgactt gatcggacct
      361 ttaccgtcga tcggttgacc gatcgcattg acgacgcgac cggccagcgc ttcgccgacc
      421 gggatttccg caatacggcc cgttcgtttg accggatcgc cttccttgat cccaacgtcg
      481 tcgcccatta acacggcccc gacgttgtct tcctcgaggt tcagcgcgat cccgtagagg
      541 cctccgggaa actcgagcat ctcaccggcc atggcaccat cgagtccgta gaccttagca
      601 atcccgtcgc cgacctggat gaccgaaccc gtttctttga catcgacctg cttatcgaag
      661 cctttgatct tttctttaat gatcgcactg atttcttctg ccttgatctg catggctact
      721 ccttggtcaa cagactctgc atggctttca gtctcccgcg gacggtgcta tccacgacgg
      781 tgctgccgat gtgaatttgg agtccggcaa ggtagctggc gttagtttca aaggtgacgt
      841 ccacgtcgcg cttcagcgtg tcgcgcagac gggtcgtaat tcgatcttgt tcagccgggg
      901 gtagcgccat tgcggatgag accgtaatct gctgagtccc ctttgactga tccaccagtt
      961 tggcgaaggc ttctgcgatc tcgggcaaga aaccgatccg gttcttcttc accaactgac
     1021 tgataaacgc tttgcccacc ggagggcacc ccagacgccc gccgagttcg gacaaaacgg
     1081 cgattttctc atcgacgccg aacgccggtg aggccaccac atggcgcaga gaaattgaat
     1141 ccttcatggc ctgactgaga ccattgagag taccccgcgt cgcttcgatg ttcgattgat
     1201 cgagaagctc gaagagtgct tgggcgtaac gtcgcgcaac tgctgtcttt atcactgtcg
     1261 tggtccgctc atgttcattt aggcatagga caaaccctcg tgcgatgcgc acgaaaatcg
     1321 gcgcgcaaag ttagcattgg ctgataggaa ctgtcaatac aggacggaca agttcctctc
     1381 gttagaatgg ctggatgatt ccgccgccga tgacttggtc gccacgataa aacaccgcgg
     1441 actgacctgg actcagggca cgttgcggaa catggaagtg gatgcggagg gattctgtgc
     1501 cgatcggctc gactcgagct ggcgtggctg gcgtggcgta gcgaaccttc acgtcgacat
     1561 cggtcgtgga ggcaaggaga ctacggtcga agaggttaag atcgttcaca tcgcagctgg
     1621 agcgaaggag tgaatcttcg gggcccaaga cgaccgtatt cgtcgttggc tgaacctcct
     1681 gaacgtagag acgctggctc gccgcgatgc ccagaccgcg tcgttgtcct ggcgtataga
     1741 acgcgattcc ttcgtgctcg ccaagctgcc gccctgcttg atcaatgaac gcaccaggct
     1801 tcttggcctc aggcatttct gcctcgataa acgtccgata gtcaccgtga ctcacgaagc
     1861 agatctcttg actctccttt agttcgtcgg ccggcaggcc aagtgattcc gcttccctcc
     1921 atacttcagc cttctgcata gcgccgacag gaaatatcag atgaggtagc cagtcaggtt
     1981 ggagtcgata gagaaaataa ctctgatcct tcctgctgtc tgcagatcgg tataaccggt
     2041 gaccggcatg gctttcggaa atgcgaacgt agtgccccgt agccacatgc ggaatgccac
     2101 gcgcctgagc aagatcgatc aacccgcgca gcttgaccct ctcgttgcat cgcacacagg
     2161 ggtttggcgt agtgccgcca gcatagccat gaagaaaatc ctcgatgact ccgctgcgaa
     2221 agttctggcg actgtcaata acctcatagg ggatgttcag ttgctgcgca acaaacctcg
     2281 cgatcccgac tttacaacag ccccgttcct gccatttctt agaggcagcc actgtttcgt
     2341 cttcatgctc ccacacttgg agagtcacac cacgcacgtc gtaaccctgg cgtaccaaca
     2401 gggctgccgc cacagagctg tctactccac cactcatacc cagaagaacg gctggacgtt
     2461 tcatagcgag cgtattgtac tgataggaga tggaaagggc tagaggctag accggaaggg
     2521 gtcgggcgct gcgctgtgcg gtttattctg tcaggaggcg atcgatatag cgcgtggttc
     2581 ctcccacgac tgcatcccca tatcgcacat gccgtgaaaa tgggaccctt cttcaatggc
     2641 aatcgatggg gttcggatat caccgaccaa gactcccggc ttgagtattt gaatcctctc
     2701 cacggcggtt atggtgccat tgatcttccc gctggtcatc aacactcctg cagacaccac
     2761 acccttgatg accgcgtgtt ctccgacgat gagggtgccg gacgtgtgga tttccccttc
     2821 tacgcggcca tcaacgcgga ccgttccatc aaaattcacc acacccttaa aatcgacatc
     2881 ttttgcaagc aacgtgaagt tctcgtagtc tggctccgcg tgcagaacct gctctttttc
     2941 tttacgtgcc cacatatgct ctccttcctt acctcaacca agaccttgtt tggctccttg
     3001 gtgatataag tatttataac taggtatatt gctgcttatc cgttaatccg cttgatgccg
     3061 ggagatgtga tgcttgcaac agaatgcagg gtcgttccct gcggcaattc ccggactccc
     3121 tgactcatct cgagtccgcc attgaagaga acgccatctt ccatggacag catgggagtc
     3181 ttcatgctcc cgttgaacac ggctggagcc cgaagtttca ctcgctcctt tgcgataata
     3241 tctccggtga tttttccctt gcacacaatc gtaccggctg tgactttggc ttggatgatg
     3301 gcttcttccc cgatcaataa aattccgtcg gtatggattt cgccatccag gtagccgtcg
     3361 atcctgacgg ttccgctgta ggaaatgatg cccttgaatt cgactccctt cccgacaaag
     3421 gcgatcacat ctccagatcc ttccgtgggg ggtgccggt
//
LOCUS       DATIFR010000010         3696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_961244, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000010 DATIFR010000000
VERSION     DATIFR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961244"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VLD60_00365"
     CDS             complement(<1..513)
                     /locus_tag="VLD60_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HSB43428.1"
                     /translation="MTSRFILPLHDCHDASLVGGKAVGLARLLASGIDVPEGFCLTTE
                     AYRLALRALGFSATERWKQALGQVAESRRLVLVECQSMIRQVDISSLVKACAEEMQRI
                     GGLERRWAVRSSASNEDTGQASFAGLYRTELGVLPTEIGRVIKELWASIWDERVVEYM
                     TKAGPDDNPPA"
     gene            complement(528..1865)
                     /locus_tag="VLD60_00370"
     CDS             complement(528..1865)
                     /locus_tag="VLD60_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSB43429.1"
                     /translation="MAIQLPASPVSPLLGKMIRRALDAVNAGAALRRAVKKDGDVLTV
                     GSRRYDLRHYERVVVIGAGKATASMARAMEQVLGSRLHSGFVIVKYGHAVPTTRIVVE
                     EAAHPLPDRAGQLAAGRVHALASSLTRHDLLIVLLSGGASSLMPSPVRGVTLADKQRV
                     TRQLLRSGASIGEVNSVRKHLSNLKGGRLAETTKATVVTLILSDVLGDDLSTIASGPT
                     APDPTTYRQAIDCLKRYHVWSSSPRAVQAHLEQGRRRHLSETPKPGSPVFRRVHHQII
                     GNSKLALAAVIRTAREAGLRTVMHSMTLAGESGVAGARFGKLARTLYAKRSSVSKSCC
                     IVAGGETTVTVKGNGKGGRAQEFAVSAAKAIAGLPNVWVAAIGTDGTDGPTDVAGALV
                     SGETLARAQRLGVDLDEALARNNTYPALRALRSHIITGPTGTNVNDLYLLLIL"
     gene            complement(2155..3216)
                     /locus_tag="VLD60_00375"
     CDS             complement(2155..3216)
                     /locus_tag="VLD60_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB43430.1"
                     /translation="MRGWLLRLLLIVTLVLAAVFPISTKAQPEPDQAPPKEAPARTGA
                     NGELDLILPEPLEGQNEPEDRLVILPEIKREGERFFLSSFKLPDKITFAGQPVPLDNW
                     QVRERIEYEFYQFLEDQGESIILAKRTGRCFPPAERQLAEAGLPDDLKYMLLVESKCI
                     SAAYSKAKASGPWQFIPSTGRRYRLKSDSVRDERRNLEMSTEAAVKYLKYLKDFQQND
                     WFLAMASYNAGEERVRKLLKEQKITDYWRMHGPRETMRYVPRIIAAKEIYSQPEKYLG
                     LSKKDLYAPLEVETVTVNVKESQRALTSIAEEFGTYFLELKMLNPEFKKDVLPHGTYE
                     IRVPRQTCPSRCFKQDKTP"
     gene            3335..>3696
                     /locus_tag="VLD60_00380"
     CDS             3335..>3696
                     /locus_tag="VLD60_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HSB43431.1"
                     /translation="MRSLAILNDRIISCTACPRLVTYRQAVAKEKRKQFRDWTYWGRP
                     IPGFGDADARLYVLGLAPAAHGGNRTGRIFTGDRSGDWLYEALHRHGFANQPTSQHKD
                     DGLALSDCYIGATVRCAPP"
BASE COUNT          772 a         1110 c         1049 g          765 t
ORIGIN      
        1 ggcaggaggg ttgtcatcag gtccggcttt ggtcatgtac tccacgactc gctcatccca
       61 gatcgatgcc cacagctcct tgatcactcg accgatctcc gtggggagca cccctagttc
      121 tgttcgatac aacccagcga agctggcttg tcccgtatct tcatttgagg ccgacgaccg
      181 cacggcccac cgccgctcca gtccaccaat cctttgcatc tcctctgcac atgccttgac
      241 cagtgacgag atatcaacct gtcgaatcat cgactggcat tcgacgagaa ccagccgacg
      301 tgattcagca acctgaccga gcgcctgttt ccaccgctct gtcgccgaga accccaatgc
      361 gcgtaacgcg agacgatacg cctccgtcgt caggcagaag ccctccggga catcaatccc
      421 ggatgccaga agtcgggcga gcccaacggc cttccccccg acgagggagg catcgtgaca
      481 atcgtgcaat ggaagaatga accgcgatgt catgctgcgg atagtaccta gaggatgaga
      541 aggaggtaaa ggtcgttgac gttcgttccg gttggtccag tgatgatatg gcttcggagg
      601 gcccgcagcg ccggatacgt attgttccgt gcgagggctt catcgagatc cacgccaagg
      661 cgttgggccc gggcgagcgt ctctccgctc acgagcgcgc cggccacatc ggtcggccca
      721 tcggtgccat cagttccaat cgctgcgacc cagacattcg gtagcccggc aatggctttt
      781 gctgcactga cagcaaactc ctgcgcacgc ccgccctttc catttccctt gacggtcaca
      841 gtggtttccc ctcctgcaac gatgcagcag gatttggaga cagaagatcg cttcgcgtac
      901 aaggtccgcg cgagtttacc gaaacgagct cccgcaactc ccgattcacc cgccagtgtc
      961 atggaatgca tgacggtccg gaggcccgcc tcccgagccg tgcggatgac ggccgccagg
     1021 gccagtttgc tgttgccgat aatctggtgg tgaacccgac gaaataccgg tgaccccggc
     1081 ttgggggttt ccgagaggtg ccgtcggcgg ccttgctcca aatgcgcctg cactgcccgt
     1141 ggagatgaag accagacgtg ataccgtttc aagcaatcga tcgcctgcct gtaagtggtc
     1201 gggtcaggtg cggtcgggcc ggaggcaatg gtactgagat cgtcgccgag gacatccgat
     1261 aagatcaagg tcacgacggt cgccttcgtc gtttcagcca ggcggcctcc cttcagattc
     1321 gacaggtgct tgcgaacact gttgacctcg ccgatgctgg ctccgctacg aagcagctgc
     1381 ctcgtcaccc gctgtttatc cgccagcgtg acgccgcgga ccggcgacgg catcaagctg
     1441 gacgcgccgc cagacaataa gacgatcagc agatcatgcc tggtcagaga ggaggccagg
     1501 gcgtgaacac gtcccgccgc cagttggccg gctcgatcag gaaggggatg ggccgcttct
     1561 tccactacga ttctggtcgt cggaaccgca tggccatatt tgacgatgac gaatccgcta
     1621 tgtaatcgac tgccaaggac ctgctccatc gcgcgggcca tcgaagccgt cgcttttccc
     1681 gccccgatca cgacgactcg ttcataatga cgcaggtcat accggcgcga cccgaccgtc
     1741 aagacatcgc catctttttt gacggcccga cgcagggcgg cgccagcatt caccgcatcc
     1801 aaggcccgcc tgatcatctt gccgagcaac gggctgaccg gagaagcagg aagctgaatc
     1861 gccatgagta gagaaacgct ctagcaggtt gcagaaacac tagtgtttcg atgaacaagg
     1921 cttcgtgaag tccagcgcgg caacagaatc cgagcagcac gcaggatact caaaaattcc
     1981 gtccagcaag gccgcagcga gcgatgaggc gaggaggtac ataccaagct tcgcttgggc
     2041 cgctcgcttc gatcacatgc gagcggatag ttactttgcc tcaagcgggc ttctccgttc
     2101 gtggagcctc tgagcaacgc gagaacgacg ctggcggact ttttcagcat cctgctaggg
     2161 cgttttgtct tgcttgaaac agcggctggg acaggtttga cggggaaccc tgatttcata
     2221 ggtcccatgc gggagcacat ctttcttgaa ttcgggattg agcatcttga gttcgaggaa
     2281 gtaggtgccg aactcctcgg cgatggaggt caacgcccgt tgtgactctt tgacattgac
     2341 ggtaacggtt tccacctcaa gcggggcata gaggtccttt ttgctcaggc ccagatactt
     2401 ctccggttgg gaatagattt ctttggccgc gatgatgcgc ggcacataac gcatggtttc
     2461 gcgcggaccg tgcatcctcc aataatcggt gatcttttgc tccttaagca gcttgcgaac
     2521 acgttcctca cctgcattat aggaggccat agccaggaac cagtcatttt gctgaaagtc
     2581 tttgagatac ttcaggtact tgaccgccgc ctccgtcgac atttccagat tgcggcgctc
     2641 atcgcgaacg gaatcgctct tgagccggta gcgtcttccg gttgacggaa taaactgcca
     2701 ggggccggat gctttcgctt tcgaataggc ggccgaaata catttgctct cgaccagcaa
     2761 catatacttc agatcatccg gcagaccggc ttcggccaac tgcctctcgg caggagggaa
     2821 acagcgtcct gtgcgtttgg cgagaataat gctttcgcct tgatcctcca gaaattgata
     2881 gaactcgtat tcgatccgtt cccgcacctg ccaattatcc aggggcaccg gttgcccagc
     2941 aaaggtaatc ttgtcgggaa gcttgaacga actgaggaag aatcgctcgc cttctcgctt
     3001 gatttccgga agaatgacaa gacgatcttc cggctcgttc tgcccctcaa gcggctcagg
     3061 aaggatcaag tcgagctcgc cgttagcgcc ggtgcgcgca ggagcctcct tcggtggagc
     3121 ttgatccggc tctggttggg ccttcgtcga aatgggaaat acggccgcca ggaccagcgt
     3181 cacaatcagc aacagacgaa gaagccagcc ccgcataacg atcctgcctc cggattgatt
     3241 ggattcactc accttcaatg tttttatgct agcagcaagc agtccgtgcg gcaagcagga
     3301 tcagctttcg cagtcgtgct acactgcggg atccatgaga tcactggcga tcctcaacga
     3361 tcgaatcatc tcctgtaccg cttgccctcg cctcgtcacc taccggcaag cggtcgcgaa
     3421 agaaaagcgg aagcagtttc gagactggac ttattgggga cggccgattc ccggtttcgg
     3481 agacgccgac gcgcgcctct acgtgctggg gctggcgccg gccgctcatg gcgggaaccg
     3541 aacgggtcgc atctttaccg gagaccgaag cggagactgg ttgtatgaag cgctgcatcg
     3601 ccacggcttt gcgaatcaac cgacatcgca gcacaaagac gatggcctcg cactatctga
     3661 ttgctacatc ggggccacgg tgcggtgcgc cccgcc
//
LOCUS       DATIFR010000011         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2302951, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000011 DATIFR010000000
VERSION     DATIFR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302951"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VLD60_00385"
     CDS             complement(<1..433)
                     /locus_tag="VLD60_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB43432.1"
                     /translation="MTDYNVLGNLLVIFTVSIAVVFVFHQFRLPSIAGFLVAGALIGP
                     HGLNLISDIGTVQVLAEIGVVLLLFTIGIEFSLLQLASLRRLLFVAAPMQVGGVLLVA
                     WVGSMAVGLTWQEGIFWGFLLSLSSTAIVLKALADKGDSDSI"
     gene            550..1266
                     /locus_tag="VLD60_00390"
     CDS             550..1266
                     /locus_tag="VLD60_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSB43433.1"
                     /translation="MQAIIFDFNGVIADDETPHFFCFRQALKESGLSLTSDEYYGTYL
                     GMDERTCTRILLTHRDGVCNDDLLERVTLRKAELFRIHTANHRPEFFPGVVEFVQAAG
                     RRYRLAIASGGRRSQIDAALGDTPIEQVFEVIVAAEDCPVGKPDPAIYRLTLERLNRS
                     SRTTSLLGPRNCFVIEDSLAGIQSAQAAGMPVLAVATTYPIEKLNGANLVTRDLRQIP
                     PDSLLERMAQISNGSHSEGS"
     gene            1407..1982
                     /locus_tag="VLD60_00395"
     CDS             1407..1982
                     /locus_tag="VLD60_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43434.1"
                     /translation="MRPLSLSILVAAIALAMSSIPASAETTEHFDPAKMWECPQADGT
                     SMYTNKERAGCKLMDLKPLSIVPSLDNMPTYRPAVAMAPHYEHPGYVHPNQSGMQDGA
                     QNVPDWARDWQASVASNGSVRAEVCSMYGEWLNLNQKTRGGFFFGSDPSYGGDLSGRN
                     QRGASYSYYDNARYHALSKIFGTGFVPVGCP"
BASE COUNT          480 a          575 c          567 g          404 t
ORIGIN      
        1 ggatggagtc gctgtcgcct ttgtcggcca aggcttttag aacgatcgct gtgctgctta
       61 gcgaaaggag aaagccccaa aagattcctt cttgccacgt gagccccacc gccatcgatc
      121 ccacccaggc cacgagcagc acgcctccca cttgcatcgg tgcggccaca aacagcagcc
      181 ggcgcaagga cgcgagctgc aacaacgaaa actcgattcc gatggtgaac agcaacagga
      241 cgacaccgat ctcggccagg acctggacag tgccgatatc ggagatgaga tttaatccgt
      301 gtgggccgat gagcgcgccg gccacaagaa agccggcaat ggatggaagt cgaaactgat
      361 ggaagacaaa gacgacggcg atcgataccg tgaagatgac gaggagattg ccgagaacat
      421 tgtagtcagt catggcaaag gtttggtgag cggagaatac cctagcctga tgaatcgaac
      481 aaggtgagac tgcttgtcct gcgtcagaca gttcagtaca gtgtgccaac cgccaggtgg
      541 ccgagcgtga tgcaagccat tattttcgac ttcaacggtg tcatcgccga cgacgaaacc
      601 ccccacttct tctgcttccg gcaagccttg aaagaatccg gtttgtcgct gacgagcgat
      661 gaatattacg ggacctattt aggcatggat gaacgtacct gtacacgtat cctgctgacc
      721 catagggacg gggtttgcaa tgatgaccta ctcgagcgcg tgaccttgcg caaggcggaa
      781 ctgttccgca tccataccgc gaaccacaga cctgagttct ttcccggcgt cgtcgaattt
      841 gtgcaggcgg ccgggaggcg ctaccggctg gcgattgcgt ccggagggcg acggtcacag
      901 atcgatgcgg ccctcggcga cactccgatc gaacaagtct tcgaggtaat tgttgctgcc
      961 gaggactgtc cggtcgggaa accggacccc gcgatctatc gtctgactct tgaacgactc
     1021 aatcgctcaa gtcgcaccac gtccctcctg ggcccccgga attgcttcgt catcgaggat
     1081 tctcttgcag ggatacaatc cgctcaagcc gccggcatgc cggtcctcgc ggtggcaaca
     1141 acctatccca ttgaaaaact gaatggagca aatcttgtta ccagagattt gcgacaaatc
     1201 ccgcctgatt ccctgctcga gaggatggct caaataagca atgggagcca ttcagaagga
     1261 tcatgagtcg gtagggaaga ccccctccaa agacgaatag acggctttca cgccgctagc
     1321 ataacttgtc atcaacgacg ccttgtgcca tcgaacaaga aaggcgactc acgatgtctt
     1381 tgctcaagtt tcaagggagg tgctttatgc ggccactctc gttgtccatc ctggtcgccg
     1441 cgattgcgtt ggccatgtct tcaatccctg cctcagcgga gacgactgaa catttcgatc
     1501 ccgcgaagat gtgggaatgt cctcaagcag acggtacatc gatgtatacg aacaaggaac
     1561 gggcgggttg caaattgatg gacctcaagc cgctgtccat cgtgccgtca ctcgacaaca
     1621 tgccgaccta tcgcccagca gtcgccatgg caccgcacta tgaacatccc ggctatgtcc
     1681 accccaacca gagtggcatg caggacggag cgcagaacgt tcccgactgg gcaagggact
     1741 ggcaggcgag cgtggcatcg aacggatccg ttcgtgccga agtctgttcg atgtacggcg
     1801 aatggctcaa tttgaatcag aaaacccgcg gcggattctt ctttgggtcc gacccctcct
     1861 acggtggaga tctctcggga aggaatcagc gaggagcgag ttactcctat tacgacaatg
     1921 cccgttacca tgccctgtct aagatctttg gaacgggctt tgtgccggtc gggtgcccct
     1981 aagccaccat acgaatgacg cgtgaacggg gcgtcgagcc gaagcc
//
LOCUS       DATIFR010000012         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_578854, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000012 DATIFR010000000
VERSION     DATIFR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578854"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLD60_00400"
     CDS             complement(<1..259)
                     /locus_tag="VLD60_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB43435.1"
                     /translation="MVRLALKNPYAVVAISLIVIILGVVSYQKMVVDIFPEINLPVVA
                     VATFYKGMGPSEVEGAITLRLEQLFLQASYVEHIESRSLPGV"
     gene            complement(317..>3395)
                     /locus_tag="VLD60_00405"
     CDS             complement(317..>3395)
                     /locus_tag="VLD60_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB43436.1"
                     /translation="VEGAITLRLEQLFLQASYVEHIESRSLPGVSLIKIYFHPSYDVN
                     AGLAEITSLTYSGLRYLPQGIFPPVIIKFGAASLPIAVQTTSSETLGEKEVRDLAYFN
                     VRPQLGNVPGIAFPTTFGGTVRQITVFLDPERMQARGISTQEIVNAVNDQTALLPAGN
                     VKIGDFDYNVYTNSMIKIVEEMNDIPLKVVNGVPITLRDVGKAVDSTQIQTNVVRIND
                     RRAVYLPIMKQAGANTIQVIDGIKEVLPKLVGLPKELIVKLLFDQSIYIRQAIQTLEH
                     EGLLGGGLACLMVLIFLGSLRSTVIIAMAIPLSVTAAFIGLYFTGHTVNIMTLGGLAL
                     AVGRLVDDAIVVVENFHRHLEMGKSSMQAAGDAVTEVALPMLVITVTVFLVFLPIAFF
                     TGVIKFLFVPLALAVAYSMVASYLVALTVAPVAMERLLRTGYGHAHESSRRSVFSGLQ
                     EFWDRLRFFDVFVEHYVSVLRWCLTHKAVVVVSVTAVFVGSLLMAPKLSTEFFPKVDA
                     GSFILNVSAPEGTRVENTEAIITQVEALIREAVPSEELDQIVSNIGLPQGWMVLYTPV
                     NGPHQAFVLVALKRDHTIKTDAVISKLRSRLIQELPGLKFSFQTGGIVSDVINFGLPA
                     PIDIKVSGPNLTDLADTANQIRNRVALIPGTTDVQVRQGMDYPEVHLDIDRSRASYVG
                     LQEKGIVSDLITGLSSNVTLNPGYWIDPRSNNAYFVVTQFPEQTLVKFEDFLNTPIVG
                     ARVGHPAATTMGGNQLLGSSLASGQTPFPERVRLPGIERESGAPIMLRDLAQVTRQSG
                     PETVDHYNLQRTMDVLVSVKGNDLGSVAGQVEEVVSQINHPKDVTVTFKGEVDSMRAA
                     IAGFGGTLPLAMVFIYLVMVGLFRSWLDPFVIMFAVPLGFIGVIWMLLLTNTSVNVES
                     MIGTLMMIGIVVSNSVLLVDFANNRLREGMPLEEAVVEAGRLRIRPILMTSLATVIGL
                     APMALGIGEGSEANMPLARAVIGGLTVSTFMTLLFIPVLHSIARRRSGSIAAP"
BASE COUNT          814 a         1012 c          882 g          687 t
ORIGIN      
        1 tcacacccgg cagtgaccgg gattcgatat gctcgacata cgaggcctgc agaaacagct
       61 gctccaggcg cagcgtgatg gccccttcga cttccgaagg ccccatgccc ttgtagaagg
      121 tcgcgaccgc cacgaccggc agattgatct ccgggaagat gtccaccacc atcttctggt
      181 aggacaccac gcccagaatg atgacgatca ggctgatcgc cacgacagca taagggtttt
      241 taagagccag acgaaccatc gtatattatc ggcttctcag gagcttctgc accaagtgat
      301 caagctccgg catgaattac ggcgccgcta tggacccaga tcgtcgacgt gcgatcgaat
      361 ggaggacggg gataaagagc agcgtcataa aggtcgacac tgtcagccct ccgatcacag
      421 cacgtgccag aggcatattc gcttcgctcc cctctccgat cccgagcgcc atcggggcga
      481 gaccgatgac ggtggctaac gatgtcatca aaatcggcct gatccgtaaa cgccccgctt
      541 ccacgacggc ttcttcgagc ggcatccctt cacgcaaacg attattggca aaatctacaa
      601 gcaatacgct attcgagacg acgatcccga tcatcatcag cgtgccgatc atggactcga
      661 cattcaccga tgtgttggtc agcaagagca tccagatgac tccgatgaat cccaatggaa
      721 cggcgaacat gatcacaaac gggtccagcc acgacctgaa caagccgacc atgaccagat
      781 agatgaacac catcgccagg gggagagttc cgccgaatcc ggcgatagct gcccgcatgc
      841 tgtcaacctc gcccttgaag gtcacagtga catccttcgg atgattaatt tgtgaaacga
      901 cttcctcgac ctggccggcc acgctcccta gatcgttgcc cttcacgctc accaacacgt
      961 ccatggtccg ttggagatta taatgatcga cggtctcggg tccggattgc ctggtcacct
     1021 gagccaggtc ccgaagcatt atcggtgcac cgctctcacg ctcaatacca ggcaaccgca
     1081 cacgctcggg aaacggagtc tgtccagagg ccaacgaact gcccagcaac tggttccctc
     1141 ccatcgtcgt cgcagcggga tgaccaaccc gcgcacccac gatcggggta ttcaagaaat
     1201 cctcaaattt caccaacgtc tgttcgggga attgtgtcac gacgaagtag gcgttattcg
     1261 aacgaggatc gatccaatac ccaggattca gcgtgacatt cgagcttaaa ccggtaatga
     1321 gatccgacac gatgcctttt tcctgaaggc caacgtacga cgctctggaa cgatcgatgt
     1381 ccagatgtac ctcaggataa tccattcctt gcctgacctg cacatcggtc gttcccggaa
     1441 tcagtgccac tctgtttcga atctggttgg cggtgtcggc taagtccgtg agattcggtc
     1501 cactgacttt aatgtcaata ggagccggga gtccgaaatt gatcacatca ctcacgatac
     1561 cgccggtctg aaaggagaac ttgagcccgg gcagttcctg gatgagacgc gagcggagtt
     1621 ttgagatcac cgcatcagtt ttgattgtat gatcccgctt gagtgcaacc aggacaaatg
     1681 cctggtgcgg cccgttcact ggcgtataaa gaaccatcca tccttggggg agcccgatat
     1741 tggagacaat ttgatccagt tcttctgagg gaaccgcctc gcggatcaag gcttccacct
     1801 gggtaatgat ggcttcggtg ttctcaaccc tcgtcccttc cggcgctgac acattaagga
     1861 tgaacgatcc tgcatccacc ttcgggaaaa attcagtgga cagcttgggt gccatgagca
     1921 aagagcccac gaacactgcc gtcacgctta caaccacgac tgccttatgc gtcaggcacc
     1981 aacgcaacac actgacatag tgttccacga atacgtcgaa aaaacgaagc cgatcccaaa
     2041 attcctgtag cccgctgaac actgaacgac gggaagactc atgcgcgtgc ccatatcctg
     2101 tccgcagaag ccgttccatc gcgaccgggg ccacggtcaa ggcgaccaga taggacgcaa
     2161 ccatcgaata ggccacggcc agtgcgaggg ggacgaacaa aaatttaatc acgcctgtaa
     2221 agaacgcgat cgggagaaat accagaaaca cggtaacggt gatgaccagc atcggcagcg
     2281 ccacttccgt cacggcatct ccagccgcct gcatggatga ttttcccatt tccagatggc
     2341 gatggaagtt ttcaactacc acgatcgcat catccaccag gcgccccacc gcaagggcca
     2401 gaccgccaag ggtcatgatg ttgaccgtgt gccccgtgaa atacagcccg atgaaagccg
     2461 ccgtcaccga tagagggatg gccatggcaa tgatgacggt ggagcgcagg ctgccgagaa
     2521 agatcagcac catgaggcag gccaatccgc cgcccaacag tccttcatgc tcgagcgtct
     2581 gaatggcctg ccgaatatag atcgattggt caaatagtaa cttgacaatc agctcttttg
     2641 gcaaacccac gagtttcggc aggacctcct tgatcccatc gatcacttga atcgtgttcg
     2701 cccctgcctg tttcatgatc gggaggtaca cggctcggcg atcgttgatc cgaacgacgt
     2761 ttgtctggat ctgggtggaa tccaccgcct ttcccacatc ccgcaaggtg atcggcaccc
     2821 cgttgacgac tttcagcggg atgtcgttca tctcctccac gatcttgatc atgctgttgg
     2881 tatagacgtt atagtcgaag tctccaatct tcacgttgcc cgcaggcaaa agggccgttt
     2941 gatcgttgac ggcattgacg atttcctggg tcgagatgcc gcgggcctgc atccgttccg
     3001 gatcgagaaa gacggtgatc tgccgcacgg tcccgccaaa ggtcgtcgga aaggcaatcc
     3061 ccggtacatt gcccaactgg ggccgcacgt tgaagtaagc cagatcccgg acctctttct
     3121 cccccagtgt ctcgctgctc gtcgtctgga cagcgatcgg caagctcgcc gccccaaact
     3181 tgatgatcac cggcggaaaa atcccctgcg gcaggtaccg cagccccgag taggtcaggc
     3241 tggtaatttc ggccagcccc gcattcacgt cgtacgacgg atggaaataa atcttgatga
     3301 gactcacacc cggcagtgac cgggattcga tatgctcgac atacgaggcc tgcagaaaca
     3361 gctgctccag gcgcagcgtg atggcccctt cgact
//
LOCUS       DATIFR010000013         1714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_579240, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000013 DATIFR010000000
VERSION     DATIFR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579240"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1037)
                     /gene="trpD"
                     /locus_tag="VLD60_00410"
     CDS             complement(24..1037)
                     /gene="trpD"
                     /locus_tag="VLD60_00410"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSB43437.1"
                     /translation="MIREAIAQLADRISLTEKEAETVMSEIMEGAATPAQIAAYLMGL
                     RIKGETVEEIAGSVRAMRDRAVRIAIGDPLVVDTCGTGGDGRHTFNISTTTAFVVAGA
                     GLTVAKHGNRSVSSKSGSADVLASLGVKIDLSPDRVADCINEVGIGFLFAPLYHGAMK
                     HCAGPRHDMGIRTMLNVLGPLTNPAGATIQILGVYEPRLTDLLGKVLMHLGSQHCFVV
                     HGMDGLDEITLTDRTIVSEAKGGVLSNYVVTPEEFHLTRVSPRELAGGSPEDNAKITK
                     EILQGRKGPKRDIVCLNAAPALVAGRKAKNLQEGFHLACRTIDSGAAADKLARLVAFT
                     AKG"
     gene            complement(1053..1619)
                     /gene="pabA"
                     /locus_tag="VLD60_00415"
     CDS             complement(1053..1619)
                     /gene="pabA"
                     /locus_tag="VLD60_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSB43438.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVRVFRNNKIGIEQIEALKP
                     DRIVISPGPCTPNEAGISVETIRHFAGRIPLLGVCLGHQSLAVAFGGEVIRAERLMHG
                     KTSMIKHDGRTIFRQLPNPFEATRYHSLIVKRSTLPSCFEVSAETEEGEIMGMRHKTL
                     GAEGVQFHPESILTAAGKDLLRNFLKLS"
BASE COUNT          348 a          485 c          516 g          365 t
ORIGIN      
        1 atgcgggaga gaatcatggg ccgctaccct ttagccgtga aggccaccag gcgagccaac
       61 ttatcggcgg cggctccgga atcgatcgtg cgacaagcca gatgaaaacc ttcctgcaga
      121 tttttggcct tgcgaccggc gaccagtgcc ggggcggcat tcaggcagac gatgtcccgc
      181 ttgggcccct tgcgcccttg gagaatttct ttggtgatct tggcgttgtc ttccggagag
      241 ccgcccgcga gttctctcgg tgacacgcgg gtcaggtgga actcttcggg agtgacgaca
      301 taattcgaga ggactccgcc tttcgcttcc gatacgatcg tccgatccgt caaggtaatt
      361 tcatcgagtc cgtccatccc gtggaccacg aaacaatgtt gcgatcctag atgcatgagg
      421 accttcccga gcaggtccgt caaacgcggc tcgtagaccc ccaggatttg aattgtggcc
      481 ccggccgggt tggtcaacgg ccccaagacg ttgagcatgg tgcgaatacc catgtcgtgc
      541 cggggtccgg cgcaatgttt catggctccg tggtagaggg gcgcaaagag aaacccgatc
      601 ccgacctcgt tgatgcaatc ggcgacgcga tcgggagaaa ggtcgatctt cacaccgagc
      661 gacgccagaa catcagcgct gccggacttg gatgaaacgg agcggttgcc gtgtttggcg
      721 acggtcaacc cggcacccgc gacaacaaag gcggtggtgg tcgaaatgtt gaaggtatgc
      781 cgcccatccc cgccagttcc gcacgtgtcg acgaccagcg gatcaccgat ggcgattctc
      841 acggcgcggt cgcgcatggc ccgtaccgag ccggcgattt cctcgacggt ttcgcctttg
      901 atgcgcaggc ccatgagata ggcggcgatc tgtgccggcg tggcggctcc ctccataatc
      961 tcgctcatca ccgtctccgc ttccttctcg gtgagggaga tacggtcggc caattgtgcg
     1021 atggcttctc taatcatgag cggacaagcg ggttacgaca gtttgaggaa attacggagc
     1081 aggtcttttc cggcggccgt aagaatcgac tccggatgaa attgcactcc ttcggcgcca
     1141 agtgttttgt gacgcatgcc catgatctcc ccttcctccg tttccgcgga tacctcgaag
     1201 cacgacggca acgtggagcg tttgacgatc aacgaatgat accgggtggc ttcgaagggg
     1261 ttcggcagct ggcgaaaaat ggtgcggccg tcgtgtttga tcatcgacgt cttgccatgc
     1321 atcaaccgtt cggcgcgaat aacttcgccg ccgaaggcga ccgcaaggga ctgatggccg
     1381 aggcagactc caagcagcgg aatgcgaccg gcaaaatgcc tgatggtctc gacggatatc
     1441 cctgcttcgt tcggcgtgca gggtcccgga gagatgacga tgcggtccgg tttcagcgct
     1501 tcgatctgct cgatcccgat cttgttgttg cgaaagacac ggacgtcttc gcccaactct
     1561 ccgaaatact ggacgagatt gtaggtaaac gaatcgtagt tgtcgatcat gagcagcatc
     1621 ggtaaatcct gctatcagct gtcagcgctt aggactcagc acaatacaga aatgtgatca
     1681 ttcgggatct gctcggagct tatggctgat ggct
//
LOCUS       DATIFR010000014         1650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2112142, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000014 DATIFR010000000
VERSION     DATIFR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112142"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VLD60_00420"
     CDS             <1..398
                     /locus_tag="VLD60_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSB43439.1"
                     /translation="IGLEKVEIENPFLAKGVRFVADGYDGNFIRDNLERDRDNFLAHL
                     DEGQKIYRAVGDCAPAFGMIGTLIGMVQMFANVTDPSKLGPFMAVALLATFYGAAVAN
                     LLCLPIADKLHLKLVDEEINRTHSSSTES"
     gene            849..1406
                     /locus_tag="VLD60_00425"
     CDS             849..1406
                     /locus_tag="VLD60_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43440.1"
                     /translation="MGARFKEDHKFSLGSASLRQALQEMPEITEVSKHIMLEETKRGL
                     TIENVDQDGRSMFPDGAKEPFKRTRKIIQKIAPQLKAMPYRLSIVGHTASSKVPSKPG
                     YGHGNCRLTEQTPCGKSWKRKVCRLAIFLWLLARLTPIPCFQTIPPCPRPPGDNYVDE
                     GRTASTRKFDPVKGGIEGWGKALCS"
     gene            1460..>1650
                     /locus_tag="VLD60_00430"
     CDS             1460..>1650
                     /locus_tag="VLD60_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HSB43441.1"
                     /translation="MNNGGSETTAATRAGPDAPPSLIALSLGSVGVVYGDIGTSPLYA
                     FREAAMAASNDAIVTRDVVL"
BASE COUNT          365 a          472 c          484 g          329 t
ORIGIN      
        1 cgatcgggct ggagaaggtc gagatcgaaa acccattcct ggccaaaggt gtccgcttcg
       61 tcgccgacgg ttacgacgga aatttcatcc gcgataatct cgagcgggat cgcgacaatt
      121 ttctagcgca tctcgatgaa ggacagaaaa tctaccgtgc ggtcggggac tgcgctccgg
      181 ccttcgggat gatcggaaca ttgatcggca tggtgcagat gtttgccaac gtgaccgatc
      241 cttcgaaact cggcccattc atggccgtcg cgctgctggc aacgttctat ggagcggcgg
      301 tggcgaacct cctgtgcctg ccgatcgctg acaaactaca cctcaagctt gtcgatgagg
      361 aaatcaaccg cacacactca tcatcgacgg aatcttgatg atacgcgaag ccaagagccc
      421 aacgctggtg tgcgagatgc tgctcgcttc cttgccggaa aagcaccggc atacagaagg
      481 ggatgaaggg gaagaagcga aatccatacc cgcttagcgc ggcgcgtttg caggcatagg
      541 atggcgagac gtaaggaagc aggtcacggc ggcggtcatg ggtggttcgt caccttcgcc
      601 gacctttatg ggccttctgg tcagtttctt tgtgatgctc gttgcctcaa tgcagaatca
      661 agccaagctc caggtcgtgg ccggttccat gcgggatgcc ttcggcgttc agggtcgcgt
      721 gcgctattcc ggaattattg aggtcctcgg ccttcccaca tgccccaggc taaagaacgc
      781 cgcacatatc gatccgtccg gagcgtccgc gaccccatcg cccaaagaga acgatcgtca
      841 acggtcatat gggggcccgc ttcaaggagg accacaagtt ctcgcttgga tctgcgtcgc
      901 tgcgccaagc cttgcaggaa atgccggaga ttaccgaggt ctcgaagcac atcatgctcg
      961 aggaaacgaa gcgggggctc accatcgaga atgtcgacca ggacgggcgt tcgatgtttc
     1021 ccgacggcgc aaaagagcct ttcaagcgca cgcgcaagat catccaaaag atcgcaccac
     1081 agctcaaggc gatgccgtat cggctctcga ttgtggggca cacggcctca agcaaggttc
     1141 cgtcaaagcc cggatatggc catgggaatt gtcggctgac cgagcaaacg ccgtgcggca
     1201 aatcctggaa gcggaaggtg tgccgcctgg caatattttt atggttgctg gcaaggctga
     1261 caccgatccc ttgtttccag acgatccctc catgcccccg accgccgggt gacaattacg
     1321 ttgatgaagg aagaaccgcc tctacccgca aatttgaccc cgtaaaaggc ggtatagaag
     1381 gctgggggaa agccctgtgt tcttaacgat ggcgcgatga tataagcccc gccttctgac
     1441 accggcgggc caattctcaa tgaataacgg cgggagtgaa acgacggctg ccacgcgtgc
     1501 cggacctgac gcgcctccaa gccttattgc gctttcgctc ggcagtgttg gcgtcgtcta
     1561 cggcgatatc gggaccagtc cgctttatgc ctttcgcgaa gccgcgatgg ccgcgagcaa
     1621 tgacgcgatc gtaacgcgcg acgtcgttct
//
LOCUS       DATIFR010000015         5901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2499300, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000015 DATIFR010000000
VERSION     DATIFR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499300"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="rpiA"
                     /locus_tag="VLD60_00435"
     CDS             complement(<1..681)
                     /gene="rpiA"
                     /locus_tag="VLD60_00435"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSB43442.1"
                     /translation="MSAIDLDALKRAAALEAVGYVKDGMVVGLGTGSTAKHMVLALGE
                     KVKAGMTLRGVPTSHETAALARQSGITLIDSDNRWVIDVAIDGADQVDPGFNLIKGGG
                     GALLKEKIVAASAKQFIVLVDYTKQVPVLGGSFPLPVEIVPFGWGSTAREIETFTKSR
                     VVLRERNGAPFKTEAGNLIVDVHLAQIDRPRDLEIALNQIPGVVETGLFVGRTDLLIV
                     GTPHGTETH"
     gene            complement(678..1742)
                     /gene="glk"
                     /locus_tag="VLD60_00440"
     CDS             complement(678..1742)
                     /gene="glk"
                     /locus_tag="VLD60_00440"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HSB43443.1"
                     /translation="MILAGDIGGTKTHLALFDWSTERVEPVRLETFHSTDYTSLEEML
                     EEFLVPPKPPTPIDELAQTTTENTEPADEPKPEAPVKIEAACFGVAGPVIQNRCHTTN
                     LPWVIDGATIAKKFEIPHVRLLNDLEAMAHGILLLRPNELATLNAGIPPAQNQAIALI
                     AAGTGLGESILFWDGTSYRPMPTEGGHTDFAPNSDNEIELLRHVRSQYLHVSYERVLS
                     GPGLHAIYEYVRDTKKNEPTWLSEKIKAGDPAAEIAEAGLKGQAEIAKQTLDLFASIY
                     GAEAGNLVLKAMAINGLYLGGGMAPKLLSKLKDGTFMKAFTNKGRYKRLMSNIPVYVV
                     MNQQAALIGAASTAAQLGRS"
     gene            complement(1765..1938)
                     /locus_tag="VLD60_00445"
     CDS             complement(1765..1938)
                     /locus_tag="VLD60_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43444.1"
                     /translation="MVLFLVTGASNAKILTWVLEPETGADRQLSAALVNPGMGHLIWS
                     LDSPAAAPPTANR"
     gene            complement(1991..2632)
                     /locus_tag="VLD60_00450"
     CDS             complement(1991..2632)
                     /locus_tag="VLD60_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43445.1"
                     /translation="MRVPFAAPIILLLFHSLAWAAEPEVIPHLDSNDPVALREAIRLL
                     DEEVKLAARPQTYVVIDLVTNALIIKGRGVELHRLPIQQWTASRLENITSTFRLRERP
                     PVNRPKIEPAGGAERSPISLDDMPSEFSLSFTPSLTVTIEPFAPDNPWRWLAFKGQEW
                     WLWFKGRFLALVSGNAVPSTPSLQLTLTHDHAQSLAWTLTEGMPFLIRRASTQ"
     gene            complement(2658..3290)
                     /locus_tag="VLD60_00455"
     CDS             complement(2658..3290)
                     /locus_tag="VLD60_00455"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSB43446.1"
                     /translation="MNRVAISLWSSLIGLVLLTLAQSSGLPVTDASLVQPSTIDPMAL
                     HALQVRYKSLAKQLTQLMPAQPYILVDTARNHLYIKRRDQVMLDALASTGSGTILDKP
                     GETNGQWIFDTPRGEFSIQSKLINPVWVKPDWAFIEEGLEVPKTQADRIEPGVLGEYA
                     LGFGKGYFIHGTLYTRLLGKNVTHGCIRLNDDDLKSVYRLARVGTPILIF"
     gene            complement(3300..4448)
                     /locus_tag="VLD60_00460"
     CDS             complement(3300..4448)
                     /locus_tag="VLD60_00460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSB43447.1"
                     /translation="MNRDHSYIAGLTRWWGAVAFAGFLAGCAEAVPQEVIDAVESVDQ
                     ALMRLRAAEIAPSDYGSFARQWATLRTRVEAEEDLIRWPWESNDLELALRHLQEEGSR
                     TVARLTEQQETRRRSAELQLAHIENRAKLISVQVSSIDSRLVLGQKPVETDLLLKQAR
                     TFYEQGLYGQSLEASDRASRNLETQAALLSRELGRYANHHHIVRWQQLAKDTVEWSKT
                     HRAPAIIVSKAERTLTLYRNGQKVLSYPVRLGFNGIREKRYQGDGATPEGRYRITSKR
                     GQGQTQFYRALVLDYPNEADRRRFVVERKTGNIPALRGIGGQIEIHGVENELMAQTLG
                     CVMLENPHMAFLFDRVEKGTPVTIVGALHEQNAVALALANLSAHRDET"
     gene            complement(4467..5084)
                     /locus_tag="VLD60_00465"
     CDS             complement(4467..5084)
                     /locus_tag="VLD60_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43448.1"
                     /translation="MKLYKPMLPILCTMILATGCAQPPTDQINAAEQAVKDAEQSGAA
                     TYVANDYAKIEGLMGALKKEVADQDAKFALLRDYGKAEQLAATAKAEAERVKADAIKK
                     MEEAKLAAMQAYQAAQDTVKTTQELVAKAPAGKDRAALESIKTDADALKASLNEVQML
                     IDKADYLGAQTKAKAIQEKGQAVSREIETALAKIGKGKASTPKKK"
     gene            5499..5636
                     /gene="rpmH"
                     /locus_tag="VLD60_00470"
     CDS             5499..5636
                     /gene="rpmH"
                     /locus_tag="VLD60_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSB43449.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLT
                     V"
BASE COUNT         1151 a         1616 c         1728 g         1406 t
ORIGIN      
        1 atgtgtttct gttccatggg gagtgcccac aatcagcagg tccgtccggc cgacgaatag
       61 gccggtctca accaccccag ggatttggtt gagggcgatt tcgagatccc gtggacggtc
      121 aatctgtgcg agatgaacgt ccactatcag attgcccgcc tcggttttga agggagcccc
      181 gttgcgctcc ctgaggacga ctcggctctt ggtgaatgtt tcgatttctc gtgcggtgct
      241 gccccacccg aatggaacta tttcgacggg aagggggaac gaacctccca agaccggcac
      301 ttgcttcgtg taatcaacca ggacgataaa ctgtttcgcc gaggccgcca cgattttttc
      361 tttcagaagc gccccaccgc ctcccttgat taagttgaag ccgggatcga cctgatctgc
      421 gccatcgatt gccacgtcga tcacccagcg attgtcacta tcgatcaacg taatgccgga
      481 ctgccttgcc agtgcggcag tttcgtgcga agtcggcacg ccccgcagcg tcatgcctgc
      541 cttcaccttc tctcccaacg cgagcaccat atgcttcgcg gttgagccgg ttcccaggcc
      601 gacgaccatt ccgtctttga cgtatcccac agcctcgagg gctgcggcgc gtttcaaggc
      661 gtcgagatcg atggcgctca tgaccgtccc agttgtgctg cagtggaagc cgcgccgatg
      721 agggcggcct gttggttcat cacgacatag acggggatat tgctcatcaa tcgtttgtat
      781 cgccctttgt tcgtgaaggc cttcatgaag gtgccgtcct tcagcttcga gaggagtttg
      841 ggggccatgc cgccgccaag gtagaggcca ttgatcgcca tcgctttcaa gaccaggttg
      901 ccagcttcgg ctccatagat ggaggcaaag agatccagtg tctgtttcgc gatctcggcc
      961 tggcctttca gtccggcttc ggcaatttct gccgccgggt cgcccgcttt gatcttctca
     1021 gacagccagg tcggttcatt cttcttcgta tcgcggacat attcatagat agcatggaga
     1081 cccgggccag acaggacgcg ctcatagctc acgtggagat attggctccg aacgtggcgt
     1141 agtaactcga tttcattgtc gctgttgggg gcaaaatccg tgtggcctcc ttcagtcggc
     1201 ataggcctgt agctggttcc atcccagaag aggattgatt cgccgaggcc ggttcctgcc
     1261 gcaatgaggg cgatcgcctg gttctgcgcc ggtggtatcc cggcgttcaa cgtcgccagt
     1321 tcgttcggcc ggaggagcag tatcccgtgc gccatcgcct cgaggtcatt gagcaagcgc
     1381 acgtgaggaa tctcgaactt ctttgcgatg gtggcgccat cgatgaccca gggcagattc
     1441 gtcgtgtggc aacggttttg tatcaccgga ccggctacac cgaaacaggc ggcttcgatc
     1501 ttcaccggtg cttccggttt gggctcatcg gccggctccg tgttttcggt tgtcgtctgt
     1561 gcgagctcgt cgatgggagt cggcggtttc gggggaacca ggaattcttc cagcatctct
     1621 tccaaggatg tgtagtcggt gctatggaag gtttctaggc ggaccggttc gacgcgttct
     1681 gtcgaccaat caaataaggc aaggtgcgtt ttggttcccc cgatatcacc ggcaagaatc
     1741 atgtgttcct cgttagcaca caagctatcg attagccgtc ggcggcgcgg ctgccggact
     1801 atccagtgac cagatgagat gccccattcc aggattcacc agggcagccg acaactgccg
     1861 atcagcgccg gtttccggtt caaggaccca cgtaaggatt ttcgcgttac tggctcccgt
     1921 gacaagaaac agtaccacac tcgcctggct gatgacacct agagatacga caaaacaact
     1981 cagccagctg ctattgtgtt gacgcccggc gaatgaggaa aggcatgcct tccgtcaggg
     2041 tccaggccaa ggactgggcg tggtcatggg tcagggtgag ttggagtgag ggggttgagg
     2101 gaacggcatt cccggatacc agtgcgagga agcggccttt gaaccagagc caccattctt
     2161 gtcctttgaa tgccaaccat cgccagggat tgtctggcgc gaagggttca atcgtcactg
     2221 tcagggaggg ggtaaagctc agggaaaatt cgcttggcat gtcgtcgagc gagatgggtg
     2281 accgctctgc tccgccagcc ggctcaattt tggggcgatt gacaggaggt cgttctcgga
     2341 ggcggaaggt tgatgtgatg ttctcgagtc gagaggctgt ccattgttga atcgggaggc
     2401 ggtgaagttc gacgccccgg cccttaataa taagggcgtt ggtgacgaga tcaatcacga
     2461 catacgtttg agggcgcgcg gcgagtttga cttcctcatc aaggagcctg atcgcttccc
     2521 ggagcgcaac cgggtcattg gagtccagat gaggaatgac ttccggttcg gccgcccagg
     2581 cgagtgaatg aaacaggagc agaataattg gggctgcgaa gggaactctc atgggggtgc
     2641 gtgctgaggg acgcggttca gaagatgagg attggagtac cgactcttgc cagccggtac
     2701 acactcttga ggtcgtcatc attgagtctg atacagccgt gggtcacgtt tttcccgagg
     2761 aggcgtgtgt agagcgtgcc gtggataaag tagcctttgc caaaacccag cgcatattct
     2821 cccaacacac ccggttcgat tcgatctgcc tgggttttgg gaacctccag cccttcctcg
     2881 ataaaggccc aatcgggctt cacccagacc ggattgatca acttggattg gatggaaaac
     2941 tctccccgcg gggtatcaaa aatccactgg ccattcgtct cgccgggctt atcgaggatt
     3001 gtgccgcttc cggttgaggc gagcgcgtcc agcatcactt gatcgcgtcg tttgatgtag
     3061 aggtgatttc ttgccgtatc aacaaggatg tatggctgag ccggcatgag ctgtgtcagc
     3121 tgtttggcta aggacttgta gcgaacttgg agggcgtgca gggccatagg gtcgatggtg
     3181 gacggttgga cgagagaggc gtctgtgacc ggtagcccgc tgctctgggc cagcgtgagg
     3241 agcacgaggc cgatgaggct tgaccagagc gagatcgcaa cgcgattcat gggttccgct
     3301 tacgtttcat ctctgtgcgc gctgagattg gcgagcgcca gtgccacggc attctgttca
     3361 tggagggccc ccacgattgt gacgggggtc cctttctcga cccggtcaaa caagaaggcc
     3421 atatggggat tctcgagcat gacgcaaccc aacgtttggg ccattaattc gttttcgact
     3481 ccgtggattt caatctggcc gcctatccct cgtaatgcgg ggatgttgcc cgttttccgc
     3541 tcaacgacga atcgtcgccg gtccgcctcg ttggggtagt ccagcaccaa cgcacgataa
     3601 aactgtgtct gcccctgtcc ccgtttgctg gtaatgcggt atcgaccttc aggagtggcg
     3661 ccatcgcctt gatagcgctt ttccctgatg ccgttgaacc cgaggcggac aggatatgat
     3721 aacactttct gaccgtttcg gtagagcgtc aaggttcgct cggccttgct gacgataatg
     3781 gccggcgctc ggtgggtttt cgaccattca accgtgtctt tcgccagttg ctgccatcgg
     3841 acgatatgat gatggttggc gtaccgccca agttcgcggc tgaggagggc agcctgagtc
     3901 tcgagattcc gtgatgcgcg gtcggaggct tcaagagact ggccatagag tccttgctca
     3961 tagaaggtgc gtgcctgttt cagcagaaga tcggtttcta caggtttctg tcccaggacg
     4021 aggcggctat caatggaact gacttggact gaaatcagtt tggctcggtt ctcgatgtgg
     4081 gccagctgaa gttcagcaga gcggcggcgc gtttcctgct gttcggtgag ccgtgcaacg
     4141 gtccgcgatc cctcttcttg taggtgccga agagcgagct ccaagtcatt tgattcccag
     4201 ggccagcgaa tgaggtcttc ctctgcttca acgcgggttc tgagggtcgc ccactgccga
     4261 gcgaagctgc cgtaatcact cggtgcgatt tcggctgccc gcaaccgcat caaggcctgg
     4321 tcaacggact ccacggcatc aatcacctct tgaggaacag cctccgcaca gccggcgagg
     4381 aagccggcga atgccacggc cccccaccag cgggtcagtc cagcgatgta tgaatgatca
     4441 cgattcatcg aacgttcaaa caacccttat tttttcttcg gggtcgaggc cttccctttc
     4501 ccaatcttgg ccagtgcggt ttcgatctcc cgagacacgg cctggccctt ctcttgaatg
     4561 gctttagcct tggtctgcgc tcccaggtaa tccgctttat cgatcagcat ctgcacctca
     4621 ttcagcgagg ctttgagtgc atccgcatcg gtcttgatcg attcaagggc tgcccgatcc
     4681 tttccggctg gtgccttagc gacaagttcc tgcgttgttt tcactgtatc ttgggcggcc
     4741 tgataggctt gcatggcggc aagctttgcc tcttccattt tcttgatggc atcagctttc
     4801 acacgttcgg cttcagcttt ggccgtggcc gcaagttgtt ccgcctttcc gtaatcgcgc
     4861 agtaatgcaa atttggcatc ttgatccgcg acttccttct tgagggcccc catcagccct
     4921 tcgatcttgg catagtcgtt cgccacatag gtggctgcgc cactctgctc ggcgtctttc
     4981 acggcttgct cagcggcgtt tatctgatcg gtaggcggct gtgcgcatcc agtcgcgagg
     5041 atcatggtgc agagaatcgg aagcatcggc ttgtatagtt tcatgatggg tttcctcctc
     5101 caagggccga caggaagtcg gtgtttaatg cgacttcgac tcccatagtc tgaatgcctg
     5161 ggaattcgaa ggaaattcgc aagggtgatg ccagaagctc gcgagctaga tcttgtgcaa
     5221 cctcctattc ttgcaacaac ttacagtgtg ggacgattcg tagttataaa atgtctgtgg
     5281 cagtctagcc cagtcacgca aaaagatcca caaaagcgcc tcattcgaaa agggttatca
     5341 aggggttacg gcaattcgaa cttcgttctg tcccctaagg aagtcattca tttcgccatt
     5401 cacctaaggc ggggcggctt ctctgcggtg gtttgacagg gggtattgtc ccccctataa
     5461 tgccacgttc atcagaattt cttcgaagga gagagcctat gtcgtttacg tttcagcagc
     5521 cttcaaatct caagaaaaag cgcgagcacg ggtttcgcaa acgcatgagc acgaagaacg
     5581 gtcggaaggt cttggctcgt cgtcgagcga aaggacgggc ccgtttgacc gtgtaggttc
     5641 gttcatgata ccgcacggat ttcgcgagga ctcttgcctc ccctggtctt tcccaagcag
     5701 ggacgctcct ctcacagcac gatggtgagt ggtcacagcc aagcccgtgg tgcaagggtt
     5761 gaacggacgg gctcacgcac agtgacttca ctcgatgagc cgaatcatgt cacagcgcgg
     5821 aggagatagg gccctctttc tgcgagccag ccgcgacatc gagtacgtca agctgcatgg
     5881 tcgtcggatc tccacgaccc t
//
LOCUS       DATIFR010000016        21634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1541586, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000016 DATIFR010000000
VERSION     DATIFR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21634
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541586"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1145)
                     /gene="aroB"
                     /locus_tag="VLD60_00475"
     CDS             complement(3..1145)
                     /gene="aroB"
                     /locus_tag="VLD60_00475"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSB43450.1"
                     /translation="MTSVSTVPVSLGQRSYDILIKPGLLERLGERLKKLRVTGKVGVV
                     TDRHVARYYAKDVLSELRRIDVEAVPIVLAPGEQSKTLKTIEHILDVLARHRFERASL
                     LLALGGGVVGDLAGFAAAIYQRGIPFIQIPTTLVAQVDSSVGGKTGVDHRLGKNLIGA
                     FYQPKAVWIDPLTLRTLPAREWVAGLAEVIKYGVIADEKFFAYLQREMSGLLRRQPDI
                     VAAVVKRSCEIKADVVAADERESDRRRILNYGHTIGHALEALGGYESLIHGEAVGIGL
                     VQEADLACYQGICERNVVENIRDVVRAAGLSDRMPAWPPDKIWKAMLHDKKVSHGQVV
                     GVWPERIGAVRMGPIDKRVFHRWYTDSCSTVARNRSLRKDSLQRLK"
     gene            complement(1465..3423)
                     /gene="pilQ"
                     /locus_tag="VLD60_00480"
     CDS             complement(1465..3423)
                     /gene="pilQ"
                     /locus_tag="VLD60_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HSB43451.1"
                     /translation="MKPLFVSANKLCSTTRFIGALGISLIALSAHAEPTTLAEGRTEL
                     AQVEKDIAALPAQSVTKIEVRPAGEQVTVVVEGDGRLYPDATLLDESRLVVDIPAVSS
                     ALPHSVVRGNHQLLKKIRVGYHADKVRLVFDIVERPVFSLDQKGNEVLVTLKPRELES
                     IAPDVAHSGGNDGAPSWDSQETKAEPTGRIARRATRIVGSAQSKFRIQPVQMAAEAGP
                     AEKDSRSDDVVAGQTRFVGRRISLDFQQADITNILRLIAEVSGFNIVVGEGVKSKVTM
                     KLVSVPWDQALDMLLKMNGLGMIRQGTIVWVDSLTNIAKQQDEEAKAKDSKTKAEELV
                     DRVFYIRNLQAQELQTSLRQYLSPRGVMNVSQGSNALIVRDTETKLGVMKQLVDGLDL
                     EVPQVQIEARIVQADTIYARGLGVQWGFTNNNLTPSTFSQVGNITGAPYSTQASGNAA
                     PRDFLVNLPAQVGGLPVVPGVGFTFGKLVEGFALDMRLSAGELLGLTKVIAAPKVTTL
                     DKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEIGKTILLRVRA
                     TRNAVGARSNPAGPSIDRREATTQVIVRDGETMVIGGVFVDTQNNNVQGIPYLSRIPV
                     LGWLFKNKSESVSKQELLIFLTPSIVKI"
     gene            complement(3420..4058)
                     /locus_tag="VLD60_00485"
     CDS             complement(3420..4058)
                     /locus_tag="VLD60_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HSB43452.1"
                     /translation="MHRHIEENGRRQRDGGIVRGVTVVTLGVLLVMSGNLHAGSLMVQ
                     KQINPMRQDSIKMSSVPDVPRTTTPTIEPSSTGTVAQSPSQLPASEVGEIGIGFGYDP
                     SGRRDPFAPVVQELQPGKADANLPPLQRVTLTELNLIAIVWGAYGYTAMVQTPEGHGY
                     TVRRGTRIGQNNGVVSAITERGIIVQERFTDVYGKKQEREYVKLLHPKEGSE"
     gene            complement(4073..4717)
                     /gene="pilO"
                     /locus_tag="VLD60_00490"
     CDS             complement(4073..4717)
                     /gene="pilO"
                     /locus_tag="VLD60_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSB43453.1"
                     /translation="MALPSLNLDALRNVPAPQKVALLVLFVGILIAGFYFYIAEPKTA
                     NIEALQAENAKLETEIQALTIKVKHLDELVAANKQLEIELAKKKERLPPEEEAIMLLK
                     QVSDLGVRLGLDIKLWKPGAQSEDASKLFVKMPVNVEVTGVYHTAALFFDRINRLPRI
                     ITVSGLKMGSPKVEQGRIVSQTSFNLIAYAAPQEKPVAAIPAPVVAAKPVQAGK"
     gene            complement(4720..5271)
                     /locus_tag="VLD60_00495"
     CDS             complement(4720..5271)
                     /locus_tag="VLD60_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSB43454.1"
                     /translation="MIRINLLPGPKGRPAKPQYDSRAQALLGVGVILITVAGCWWYSA
                     SLDSEIEARQEEKRDKEKQVAQLKEQVKQVQDFEQKKKLLEDKNRIIDQLEKSRVGPV
                     KVLDHVSQSLEPLKVWLTRLGITNEAVEVEGKALTNDDVVEFVNNLRRTDYFTNINLQ
                     ESKATVENKINVYQFSLAFRLKG"
     gene            complement(5268..6374)
                     /gene="pilM"
                     /locus_tag="VLD60_00500"
     CDS             complement(5268..6374)
                     /gene="pilM"
                     /locus_tag="VLD60_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HSB43455.1"
                     /translation="MLTSFKNIFETDIVSLLTPRRQLVGLDIGSSAIKLVQLKESKGR
                     YFLQKFGVKPLEPEVIVDGTVMDEGRVVSAIRELFDESKVKNKHVAISISGHAVIVKK
                     ISLPPMPDEELEGQVRLAAEQYIPFDINEVNIDFHVLPPDPSGDAQGDMSVILVAAKK
                     DKINELTELVKGAGLTPMVMDVDAFAIENMHAINYPMAQDETTALVNLGASVMNVNII
                     RAGVSLFTRDIPLGGNRYTEAIQREMGMSYEEAEESKKGEGSTDVNAVSLGNVIDSVN
                     GEVASEIARTVDYFKTSMANAELDRVLVCGGVAKAKGLVQQLGDRMQTPVEVANPFAE
                     IDVTGSDFDPDALAELAPLAAVGVGLALRAVGDR"
     gene            complement(6741..7607)
                     /gene="rapZ"
                     /locus_tag="VLD60_00505"
     CDS             complement(6741..7607)
                     /gene="rapZ"
                     /locus_tag="VLD60_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSB43456.1"
                     /translation="MARLNLVIISGLSGSGKSHALKAFEDAGYFCIDNLPPALLPTFV
                     ELCNQQNGEIANVALGVDVRERVFFSDLVGILERVKGLGHSVHLLFLEAREEVLVRRF
                     SESRRPHPILPHLPVLEGVRIEKERVAELRRHADRIIDTSDLTVHELRDLLTKQFQQE
                     PTARKLTISLLSFGYKYGVPYDIDLLFDVRFLKNPYFVPDLKPLPGDDPRVRAFVLTD
                     PDAIALIGQLEGLLKFLIPHFEREQRSYLTIAIGCTGGRHRSVAVAGRLAESLSAMGY
                     EVALKHRDIQKS"
     gene            complement(7722..8291)
                     /gene="raiA"
                     /locus_tag="VLD60_00510"
     CDS             complement(7722..8291)
                     /gene="raiA"
                     /locus_tag="VLD60_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HSB43457.1"
                     /translation="MKLRITGRHMNVTPALRDYVETRFGRLDRYGLKVGSLQVVLGVE
                     KLQHKAEVVGNVNGKRVQAKTSTREMYATIDALVDRVDAQFRKWKERLVNHKPSKSRK
                     PRVSDRRTVKWGQEEPTEVEQQAVPVLSIDQAKKEFSALPATAPFLLFINAETGRVQM
                     VQRLISGRLAVIEPYPEGGRARVLSPLMP"
     gene            complement(8341..9849)
                     /gene="rpoN"
                     /locus_tag="VLD60_00515"
     CDS             complement(8341..9849)
                     /gene="rpoN"
                     /locus_tag="VLD60_00515"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HSB43458.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLAQHLLEN
                     PLLDEVQAEAEEAETASDEEKAEEAPAPTSQDQPDEGGTPEERGSPEESTASGWEEYF
                     GSDRRAGDSEYPSSSQEEFPSYEQTVAKATSLEEHLLWQLSMSGLSDREKALGRLIIG
                     NLDDDGYLRIALTEVVAGTDFTEAEAESVLKDIQTFDPTGVGARDLPECLLLQLGHLG
                     RNPIGSLGSRPGALKGSIVEGIILHHLKDLEKKQYAKIAKALNVTVEEVFQATKVIEN
                     LEPKPGRPFTNTQNYVIVPDVFVVKNEGEWVVLLNDDGLPRMRISPYYKQLMASGQGG
                     SAETKAYMDEKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEQFFEHGVQYLKPLVLKQ
                     VAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPSDMHSSVSVREMIK
                     KMVAEEDTKRPLKDEEIAARLRTQQVLIARRTVAKYRAEENIPSASQRKRFF"
     gene            complement(9868..10650)
                     /gene="lptB"
                     /locus_tag="VLD60_00520"
     CDS             complement(9868..10650)
                     /gene="lptB"
                     /locus_tag="VLD60_00520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HSB43459.1"
                     /translation="MTQGVHTGSELLAESSSSKRVGCLRASGLMKSFRGRKVVKGVAI
                     EVRAGEVVGLLGPNGAGKTTIFDMMVGLCQPDEGEITFDGESVTSLPMYKRALRGIGY
                     LPQESSVFRRLSVEHNVLAILEMLGYAREERTQRVDALLKELDLVHIRRSMAYALSGG
                     ERRRLEITRALATDPSFMLLDEPFAGIDPIAVADIQQIITRLKEKGIGILITDHNVQE
                     TLSITDRAYIINEGLILESGSPDAIVKSETARAVYLGDRFKL"
     gene            complement(10647..11243)
                     /locus_tag="VLD60_00525"
     CDS             complement(10647..11243)
                     /locus_tag="VLD60_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HSB43460.1"
                     /translation="MWIYLLLLNALVFGAAPLAHSAESGLAKKTGEAPAVSTTITAKK
                     MTVRNKDSQAVFEGTVVLTRGPLIVYSDKMVVMFHSQDNDAAQPTLGGRDRGDQTKSP
                     SLPKGSDAMPAVSNRSVSRVEATGHVKIEKENGNATCQKAVYFSQDEKIVLTGDPVAW
                     EKGTRVSGKQITMYLAEDRSVVEGGSHVRIEGEGKSAP"
     gene            complement(11218..11790)
                     /gene="lptC"
                     /locus_tag="VLD60_00530"
     CDS             complement(11218..11790)
                     /gene="lptC"
                     /locus_tag="VLD60_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HSB43461.1"
                     /translation="MWELVARRMLLAVSVILAAFLGYLLFMNADSVPTSQSAPPGSIE
                     QADARISEFTFTQSKGDVIQWQVQAKQARLFEREKRALLSDVDVTLYGAKGKELTVSG
                     EEGTLDTGTKNFVLANRADPLVVETVSGYTIYTNHLVWTDETKEIRTDDPVRIVGHGL
                     VVTGRGLLGRMQSEEFEVLEDVHVDLSPAT"
     gene            complement(11963..12394)
                     /gene="rplQ"
                     /locus_tag="VLD60_00535"
     CDS             complement(11963..12394)
                     /gene="rplQ"
                     /locus_tag="VLD60_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSB43462.1"
                     /translation="MRHRKKGRQLGRQTKHRWALFRSLVTSLLDHERIETTEAKAKEI
                     RGFTDRMISLGKEGTLPARRRALGFLRSKAVVSKLFSDVAGRFKDRPGGYTRIVKTRR
                     RIGDAAEMVAIELVSRAEPATKKKSAAAPAQSPAGESEKSA"
     gene            complement(12417..13406)
                     /locus_tag="VLD60_00540"
     CDS             complement(12417..13406)
                     /locus_tag="VLD60_00540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSB43463.1"
                     /translation="MKDFQVPMRVEVDKDAHSPTFGRFTTEAFERGFGTTIGNALRRV
                     LLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTAIILNIKSLRLALHTDKPKTIRLKK
                     KGPGEAKGSDIIHDADVTILTPDLHIATLDKDATLDIEMTIKHGRGYVPAERNKEEGL
                     PIGVIAIDSVFSPIKRVNFHVENARVGRMTDYDKLTMEIWTDATISPRDALSTAAGIL
                     REHLDIFINPEERAEGKSEAGYEESQREVNKNLFRSVNELELSVRAANCLKNANIKTI
                     ADLVQKSEGEMLKTKNFGKKSLNEIKEILSEMGLSLGTKVDATSPHNGSPKSE"
     gene            complement(13507..14133)
                     /gene="rpsD"
                     /locus_tag="VLD60_00545"
     CDS             complement(13507..14133)
                     /gene="rpsD"
                     /locus_tag="VLD60_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSB43464.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QGRPRTSDYSLQLREKQKLRRIYGLQERQFRGVFERAERQTGVTGDALLRLLECRLDN
                     VAYRLGFGASRKQARQVVSHGHLTINGKKITVAGALVKAGDVIEVRERSRNLAAIQAA
                     LESVDSRGIPDWLELDKGAFKGIVRSLPAKEQITLPVNEQMVVELYSR"
     gene            complement(14209..14592)
                     /gene="rpsK"
                     /locus_tag="VLD60_00550"
     CDS             complement(14209..14592)
                     /gene="rpsK"
                     /locus_tag="VLD60_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSB43465.1"
                     /translation="MSVKKGKKKERRIVQSGVAHVQASFNNTIVTITDLSGNTVVWAS
                     AGNQGFKGSRKSTPFAAQRAGEAAARKAMESGMRQIDVYVNGPGSGRESAIRSLQAAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(14621..15016)
                     /gene="rpsM"
                     /locus_tag="VLD60_00555"
     CDS             complement(14621..15016)
                     /gene="rpsM"
                     /locus_tag="VLD60_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSB43466.1"
                     /translation="MARIAGVDLPRDKRADIGLTYIYGIGRAAALQILKEAGVDGSIR
                     VKDLSEDKIVKLREIIERDYRVEGDLRKEVSLNIKRLVDTGTYRGLRHRKGLPVRGQR
                     TKTNARTRKGRRSGVGSKPRPTAKATPGA"
     gene            complement(15082..15195)
                     /gene="rpmJ"
                     /locus_tag="VLD60_00560"
     CDS             complement(15082..15195)
                     /gene="rpmJ"
                     /locus_tag="VLD60_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSB43467.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRILCDNPRHKQRQG"
     gene            complement(15215..15433)
                     /gene="infA"
                     /locus_tag="VLD60_00565"
     CDS             complement(15215..15433)
                     /gene="infA"
                     /locus_tag="VLD60_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSB43468.1"
                     /translation="MAKEDIIEVQGSVAETLPNAMFRVKLDNGHVILAHISGKMRMHF
                     IRILPGDKVTVEMSPYDLTRGRITYRFK"
     gene            complement(15461..16225)
                     /gene="map"
                     /locus_tag="VLD60_00570"
     CDS             complement(15461..16225)
                     /gene="map"
                     /locus_tag="VLD60_00570"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSB43469.1"
                     /translation="MIILKTPAEIEVMAEASRVVAEALEIVRKEVRPGISTDDLDRIA
                     EEAIRARGGTPAFKGYRSYPKTLCASVNEQVVHGIPSKRKLKDGDIIGLDLGAIVGGF
                     YGDSAVTVAVGRISEATAKLVQVTEEALYLGIQQAVVGNRLTDISHAVQRYVESAGYS
                     VVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQPGMVLAIEPMVNMGGSAVRVLEDRW
                     TAVTADGSLSAHFEHTIAIQPNGPARVLSQRSERAG"
     gene            complement(16222..16878)
                     /locus_tag="VLD60_00575"
     CDS             complement(16222..16878)
                     /locus_tag="VLD60_00575"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSB43470.1"
                     /translation="MQLVFLGAPGVGKGTQADKVAAQYGVLKISTGDLLREAVRNQTK
                     LGLEAKGYMDQGKLVPDSVVIGLVRDKLADPSYAKGFILDGFPRTVPQAEELGKVLNE
                     RGFRLDRVINFQVSREDVVKRLSGRRSCPKCQATYHVDFAPSKKGGLCDRCGEALIQR
                     SDDQREAIETRLRVYEEQTAPLISFYEKKNMLSHLDGAGSVEAVYQGLTKVLVAYKTA
                     "
     gene            complement(16885..18204)
                     /gene="secY"
                     /locus_tag="VLD60_00580"
     CDS             complement(16885..18204)
                     /gene="secY"
                     /locus_tag="VLD60_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSB43471.1"
                     /translation="MFERLLTSFQNIFKIPELRTRVLFTLGMLVVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVVPHLS
                     KLAKEGERGRKKIIQYTRFGTIAIALIQGFGIAIGLEQMNQGAFVLTAGWGFRLMTVI
                     TLTAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTFDLYKVGQLNIVLL
                     VALAALMVAVVAAIVFLESGRRKIPVQYAKRVIGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWVKAIGTQLAPGSLLYTLMYVGLIVFFCFFYTAVVLNPV
                     DMADNMKKYGGFIPGIRPGQRTSDYIYNVLTKITFAGAIYLAIVCVIPEFLIYKLNVP
                     FYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMAPLRGRSG"
     gene            complement(18197..18646)
                     /gene="rplO"
                     /locus_tag="VLD60_00585"
     CDS             complement(18197..18646)
                     /gene="rplO"
                     /locus_tag="VLD60_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HSB43472.1"
                     /translation="MNLHDLTPARGAKKRRKRIGRGPGSGHGKTATKGHKGLLARSGG
                     GKRPGFEGGQMPLVRRLPKFGFTNPFRTEYAIVNVKSFEKWTGVETITPQAMVDAGLV
                     KRKKLPIKILGNGELKKSLVIQAHKFSKSAEAKIQAAGGRVEVIGGV"
     gene            complement(18643..18861)
                     /gene="rpmD"
                     /locus_tag="VLD60_00590"
     CDS             complement(18643..18861)
                     /gene="rpmD"
                     /locus_tag="VLD60_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSB43473.1"
                     /translation="MGTAKPSKTSKSGTQGVRVTLKRSPIGTPERHRLVLRGLGLRHI
                     RQTAVHPDTPQVRGMIHKVGYLLEVGKP"
     gene            complement(18861..19370)
                     /gene="rpsE"
                     /locus_tag="VLD60_00595"
     CDS             complement(18861..19370)
                     /gene="rpsE"
                     /locus_tag="VLD60_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSB43474.1"
                     /translation="MRVNPDELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGHGW
                     VGIGKGKAAEVPVAISKAVEQAKKHLVHVPLKGGSIPHEVHGLFGGEHVLLKPAVDGT
                     GIIAGGAVRAVVELVGAHNVIAKTLGRGNPFNTVRATLDGLTQLRNPDEVLRLRRQTA
                     AERHERAAV"
     gene            complement(19432..19797)
                     /gene="rplR"
                     /locus_tag="VLD60_00600"
     CDS             complement(19432..19797)
                     /gene="rplR"
                     /locus_tag="VLD60_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSB43475.1"
                     /translation="MNTADKVRQLERRRQRVRHTIMGTAERPRLNVFRSSSHIYAQII
                     DDIKGTTLAAASSLDKSLRKSLKSTGSIEAAKAVGKLIADRAKAAKVTAVVFDRGGRM
                     YHGRIKALADASREGGLQF"
     gene            complement(19807..20343)
                     /gene="rplF"
                     /locus_tag="VLD60_00605"
     CDS             complement(19807..20343)
                     /gene="rplF"
                     /locus_tag="VLD60_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSB43476.1"
                     /translation="MSRIGKKPIAIPGGVEVKVSGSTVSIKGPLGKLDWSLAQGVGVA
                     VDNGQVVVSRSSDDRKLRALHGLVRAELNNMIHGVTKGYERSLEITGVGYKTQIQGRT
                     MSFNVGYINPVVYQVPTGIDVKVDKQTLINIKGADKRLVGQVAANLRAIKPPDVYKQK
                     GVRYMGEVLRKKEGKTGK"
     gene            complement(20386..20781)
                     /gene="rpsH"
                     /locus_tag="VLD60_00610"
     CDS             complement(20386..20781)
                     /gene="rpsH"
                     /locus_tag="VLD60_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSB43477.1"
                     /translation="MVTDPISDLLVRLKNGAQRRHETIAVPASKLKRAILEILTKEGY
                     VESVEDGVRDGHPVLNVHLRYVDEGRPMITGLERISKPGRRVYVGSRDITKVRNGIGM
                     SILSTSKGIMTDQESRRNKLGGEILCSVW"
     gene            complement(20833..21024)
                     /locus_tag="VLD60_00615"
     CDS             complement(20833..21024)
                     /locus_tag="VLD60_00615"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HSB43478.1"
                     /translation="MVVSRLALRNKAVTKQKFSSREYHRCGVCGRVRGYLRRFRMCRI
                     CFRFLSLRGEIPGVRKSSW"
     gene            complement(21063..21584)
                     /gene="rplE"
                     /locus_tag="VLD60_00620"
     CDS             complement(21063..21584)
                     /gene="rplE"
                     /locus_tag="VLD60_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSB43479.1"
                     /translation="MYLQQVVPALMKEFGYKNVMQVPKLERVVLNVGMGEAIQNVKLL
                     ESAVTELGTITGQKPVVTRAKKAIAGFKLRQGLPIGTKVTLRSRRMYEFFDRLVTLAL
                     PRIRDFRGVSPKAFDGRGNYTLGLKEQLIFPEIKYDEVASIHGMDITIVTTARTNDEG
                     KALLKHLGMPFRA"
BASE COUNT         4608 a         6739 c         5289 g         4998 t
ORIGIN      
        1 gcctatttca acctctgaag agaatccttg cggagagacc tgttccgcgc aaccgtggag
       61 cacgaatccg tataccaccg atggaaaact cgcttgtcta tcggccccat gcggacagcc
      121 ccgatgcgtt cgggccatac acccacaacc tgtccatgcg agaccttctt gtcgtggagc
      181 atcgccttcc agatcttatc gggcggccag gcaggcatac ggtcactcag ccctgcagcc
      241 ctcacgacat ctcgaatgtt ttcgacaaca ttacgttcac agataccctg gtagcatgcg
      301 aggtcggcct cttgaaccag cccgatcccc acggcctcgc catgaatcag cgattcatac
      361 ccgcccaacg cttcgagggc gtgcccgata gtatgtccgt aattgagtat tcgccttcga
      421 tcggactccc gttcatccgc cgctaccacg tcggctttga tctcgcacga tcgcttcacc
      481 actgctgcga caatatcagg ctgccgccga agcaaaccag acatttcccg ttgaagatac
      541 gcgaagaact tttcatcagc gatcacgcca tacttgatca cttccgcgag ccctgcgacc
      601 cactcccttg cgggcaatgt gcgaagagtc aagggatcaa tccagaccgc tttcggttga
      661 taaaacgcgc cgatcagatt cttgccgagc cgatggtcga cacccgtctt ccctccgaca
      721 ctcgagtcca cctgagccac cagcgtcgtc ggtatttgta tgaagggaat gccgcgttga
      781 tagatggccg ccgcaaaacc cgccagatct ccgacaaccc cacctcccag agccaggaga
      841 agcgaggccc gctcaaaccg gtgacgggct aacacatcga gaatgtgctc aattgtcttc
      901 agagtcttgg actgttcacc gggagcaagt acgatgggca cggcctcaac atcgattctc
      961 ctcagctcgc ttagaacgtc tttcgcgtag tagcgcgcca cgtgccggtc ggtcaccacc
     1021 ccgacttttc ccgttacgcg cagtttcttc agtcgctctc caagcctctc caggaggccg
     1081 ggcttgatca gaatgtcgta gcttcgctgg ccgagagaga cagggacggt cgataccgat
     1141 gtcatggacc aaccctgaac ggcaggtaca ggcggaattc catgagacgc cgggactcct
     1201 tcacatgcta ctgaaatatc acaataatcc tgacaggttg gaagaatgta gcacagccta
     1261 gataaaacta aaaggatggc ctccgcccgc ggatcatctc agcggtgcga aggttctgag
     1321 agagcgcctc cggctcggtg aaaccggcag agagcggtcg cattccgatg gagctcataa
     1381 aaacgaaaca gcgttgtgat gccacgcggg catcacaacg cctgtctccc ccgaagctgc
     1441 acgagggatg gatctaggaa cgggctagat cttgacgatg ctcggagtta agaaaatgag
     1501 cagctcctgt ttcgataccg attcagactt gttcttaaac agccacccga gcaccgggat
     1561 acgggagaga tacggaatcc cctgcacgtt gttgttctgg gtatcaacga agaccccgcc
     1621 gatcaccatc gtttccccgt ctcggacgat cacctgcgtc gtcgcctcac gccgatcgat
     1681 acttggaccg gccggattgc tgcgagcgcc gaccgcattt cgagttgcgc gcacgcgcaa
     1741 caggatggtc ttcccaattt ccttcgggtc acgggaggtg atctgcggcg ttacgttcaa
     1801 ctccagattg gcatccacaa atgtcgtttg cgtaccctgt aatgacgtcg tctggaaagg
     1861 aattgattca ccctgagaga tcttcgcctc tctcttatcc aacgttgtga ccttcggagc
     1921 tgcaatgact ttcgtcaaac ccagcaattc acctgccgat aaccgcatgt ccagtgcgaa
     1981 tccctcgacc aatttgccaa atgtaaagcc cacacccggc accacgggca acccgcccac
     2041 ctgagccggc aaattcacca agaagtcccg cggggcggca ttgcccgatg cctgagtgga
     2101 atacggagca ccagtgatat ttccgacctg gctgaaggta ctgggtgtca ggttattatt
     2161 cgtgaacccc cactgaaccc ccaaccctct ggcatagatt gtatccgcct gaacaattct
     2221 cgcctcaatc tgaacctgcg gaacctccaa atcgagcccg tcgacaagct gcttcatgac
     2281 cccgagcttt gtctcggtat ctctcacgat gagggcattg ctgccctggc tgacattcat
     2341 gacgccacgc gggctgaggt actggcgcaa cgaggtttgc agttcctggg cctgaagatt
     2401 cctgatatag aacacccggt caaccagctc ttcagccttg gtcttggaat ccttggcttt
     2461 cgcctcttca tcctgttgct tcgcaatatt tgtcaggctg tccacccata cgatggtccc
     2521 ctgacggatc ataccgagac cattcatctt caagagcatg tcgagagcct ggtcccatgg
     2581 cacactgaca agcttcatgg tgacctttga tttgaccccc tctccgacga cgatattgaa
     2641 accgctgacc tcggcaatca acctgagaat gtttgtgata tccgcctgct ggaaatccag
     2701 ggagattcgt cgccccacaa aacgggtctg tcctgcgaca acgtcgtcgg accggctgtc
     2761 cttttcagca ggccccgctt ccgccgccat ctgaacgggc tgaatcctga acttggactg
     2821 tgcggagccg acaattcttg tcgcccttcg ggcgatgcga cccgtgggtt cggctttggt
     2881 ttcctgggag tcccatgacg gagctccatc gttgcctccg gaatgggcca catcaggggc
     2941 aatgctttcg agctcacgag gcttgagtgt gacgaggact tcgttgcctt tctggtcaag
     3001 agaaaacacc ggacgttcca caatgtcgaa aacaagacgg accttgtcgg catggtaccc
     3061 gaccctgatt tttttcagta gctgatgatt gcctcgcacg acggaatgcg gaagcgcgga
     3121 ggacacggcc ggaatgtcga cgaccaagcg agactcatcc aacagcgttg cgtccggata
     3181 caaccggcca tcgccctcga caaccacggt gacctgctcc ccggcggggc gtacctcaat
     3241 cttggtaacc gattgggcag gcaacgcggc gatgtctttt tcgacttgcg cgagttcggt
     3301 ccttccctcg gcaagagtcg tcggctcagc gtgcgcgctc agggcgatga gggaaatccc
     3361 caaggctccg atgaaccgcg tggtggaaca caacttgttc gcgctaacaa atagtggttt
     3421 cattctgagc cctctttcgg atggaggagc ttgacatact cgcgctcctg cttcttgcca
     3481 tacacatcgg taaatcgctc ttgcacaata atgcctcgtt ctgtaatcgc gctcaccacc
     3541 ccgttattct gaccaattct tgtcccccgc cttaccgtat acccgtgtcc ctccggcgtc
     3601 tggaccatgg cggtataccc atacgccccc caaacaatcg cgatcagatt gagctccgtc
     3661 agcgtaacgc gttgcagcgg gggaagattc gcatcggcct ttcccggctg cagttcttgc
     3721 acaaccggag caaagggatc tcgtcgcccg gaggggtcat agccaaagcc gatgccaatc
     3781 tctccaacct ctgaggcagg caattgagac ggactctgcg ccaccgtacc ggtcgaggat
     3841 ggttcaatgg ttggagtcgt cgttcttgga acgtctggga cggaggacat cttgatggag
     3901 tcctgtctca taggattgat ctgtttctgc accatgagac tgcctgcgtg caggtttccg
     3961 ctcataacga ggaggacgcc gagggtgacg actgtaaccc cccgtacgat tccaccgtct
     4021 cgctgacgtc gaccattctc ctcaatatgc ctatgcattg cttctcgact ccctattttc
     4081 ccgcttggac cggcttggct gcgacaaccg gtgccggaat ggccgcaact ggtttttcct
     4141 ggggggccgc atatgcaatc agattaaacg atgtttgcga tacaatccgc ccttgctcta
     4201 ccttcgggga ccccatcttc agtccggaaa ccgtgatgat tctaggaagc cggttaatcc
     4261 ggtcaaagaa cagtgcggcg gtgtgataga cccctgtgac ttccacattc accggcatct
     4321 tcacgaacag cttagaggca tcttcggatt gcgccccagg cttccacaat ttgatgtcga
     4381 gccccaatcg aaccccaagg tcggatacct gcttcaataa catgatcgct tcttcttccg
     4441 gggggagccg ctctttcttc ttggccaatt caatctcgag ttgcttattc gcagccacca
     4501 gttcgtcgag atgcttaact tttatcgtga gcgcttgaat ctcagtctcc aacttggcgt
     4561 tctccgcctg gagcgcttca atattcgccg ttttcggttc ggcaatgtag aaatagaatc
     4621 cggcaatcag tatgccgaca aagaggacga gcagcgccac cttctgaggg gccggaacat
     4681 tgcgcagcgc gtcaagattc agtgaaggca gtgccatacc taccccttca ggcgaaatgc
     4741 caggctaaac tgatacacat taatcttgtt ttcaacggtc gctttgcttt cctgcaaatt
     4801 gatgttggtg aagtaatccg tccggcggag attgttgacg aactccacca catcatcgtt
     4861 cgtgagcgcc ttcccctcta cttcgacggc ctcatttgta atacctagcc tcgtcagcca
     4921 gaccttcagg ggctccagac tttggcttac atgatccagg actttgacgg gtcccactcg
     4981 tgatttttca agctgatcga taatgcgatt cttatcttcc agaagtttct tcttctgttc
     5041 aaaatcttga acctgcttaa cctgctcttt cagttgagct acttgctttt ctttatctcg
     5101 tttctcctct tgccgtgctt cgatttcact atccagagag gctgaatacc accagcagcc
     5161 ggcaacggtg attagaatca cgccaacgcc gagaagcgcc tgggcacgcg aatcatactg
     5221 aggctttgcc ggtcttccct taggcccggg aagtaaatta atccgaatca tcgatccccc
     5281 acagctctca acgccaaccc cacaccaact gctgccaacg gagccaactc ggccaacgcg
     5341 tccggatcaa agtcgcttcc tgtgacatca atctcagcaa aggggttggc gacctccacc
     5401 ggagtttgca tccgatcgcc caactgctgg accagcccct tggcctttgc cacacctccg
     5461 cacacgagaa ctcgatcaag ttccgcattt gccatggacg tcttaaaata atccactgtt
     5521 cttgctatct ctgaggcgac ttctccgttc acgctatcaa tgacattgcc cagtgacacc
     5581 gcattcacat cggtggaacc ttcgcccttc ttactctctt cagcctcttc gtaggacatg
     5641 cccatttcgc gttgaatggc ctcggtatag cgattgccgc ccagaggaat atcccgggta
     5701 aataaggaca cccccgcgcg aataatgttt acgttcatca cgctggcacc aagattgacc
     5761 agcgcagtcg tttcatcctg agccatcgga tagttgatcg catgcatgtt ttcgatggca
     5821 aaggcgtcga catccatgac catcggggtc aggcctgcgc ctttgacgag ttcggtcagc
     5881 tcgttgatct tatccttctt ggcagcgacg agaatgacgg acatgtcccc ttgcgcatct
     5941 ccggaagggt ctggaggcag gacatgaaaa tcgatgttca cctcattaat atcgaagggg
     6001 atgtactgct cggccgccaa tctcacctgc ccctccaact cttcatcagg catgggaggc
     6061 agactgatct tctttacgat tacggcatgc ccggagattg aaatcgccac atgcttgttt
     6121 ttgaccttgg actcatcgaa caattcccgg atggccgaca cgactcgtcc ctcatccatg
     6181 acggtcccat cgacgatcac ttccggctca agcggcttca ccccgaactt ctgaagaaag
     6241 taccgcccct tgctctcttt caactgtaca agcttgatcg cgcttgatcc gatatcaagc
     6301 cccacgagct gccgtcgagg agtgagcaac gagacaatgt ccgtttcgaa gatattttta
     6361 aatgaggtca gcatgtgtat tccctgtcac cctacacgac aatcttccgt gcgcgaataa
     6421 ctttcttgat ttcatccaca ttctttctcg tatacagcct ccagtttctc caatcacgag
     6481 gaggcccgga gatgactccc tcgcgttccc agcgaaagag ggtcgccttt gaaatgtcga
     6541 ataagtcaca cacttgattt gttttgaacc ccgtttcctt tgaagttgaa atgcgtttct
     6601 tcacgttgcg aatccgctct acgaaagccg atttcaatat gcacaaggct aagtatagtc
     6661 aatatgctct acctgtcaag aaaaccaccg gaagcagaaa tcccaggtgg aaactcggat
     6721 ccccgctgtt gcggcaaggc ctatgacttt tggatatccc ggtgcttcag ggccacttcg
     6781 tagcccatgg ctgagaggct ttctgcgaga cgtccggcga ctgcaacgga gcgatgtcga
     6841 ccaccggtgc agccgatggc gatcgtcaga tagctacgct gttcccgttc gaagtgggga
     6901 atcaggaact tgagcaaccc ctcgagttgc ccgatcaacg caatggcatc gggatccgtt
     6961 aggacaaacg cgcgaactcg tgggtcgtct cctgggagcg gcttgagatc gggcacgaaa
     7021 taggggttct tgagaaatcg cacgtcgaat aacaggtcga tgtcgtaggg cacgccatac
     7081 ttatagccaa agcttagcaa tgaaatggtc aactttctgg ctgtcggctc ttgctgaaac
     7141 tgcttcgtca gcagatctcg gagctcatgc acagtaaggt cggacgtatc gatgatccgg
     7201 tcggcgtgcc gccgaagttc ggcgacccgc tccttttcga ttcggacgcc ttccagcacc
     7261 ggaagatgcg gcaaaatcgg atgtgggcgc ctcgattccg aaaaccgcct taccaagacc
     7321 tcttcccgcg cttcgaggaa cagcaagtga acggaatgcc cgagtccctt cacacgctca
     7381 agaatgccaa cgagatcgga aaaaaatacg cgctcacgta catcaacgcc gagcgccacg
     7441 ttggcgatct cgccattctg ctggttacag agttcaacga atgtgggcag cagcgcgggg
     7501 ggaagattgt cgatacagaa atatccggca tcctcaaacg ccttgagagc gtgtgactta
     7561 ccggatccgg acaatccgct gatgatgacg agattgagcc gtgccattgc gctgctatgc
     7621 tctgatcaga tggatccgcc tcggcggaac tgaacaagcg acattcagtg gaacaggaaa
     7681 ggcaccgaag acggctgagg tgaatcgcgt tcgcactctc ctcacggcat caaaggagag
     7741 agaacacgtg cccgaccacc ctcggggtag ggttcgatta cagcaagtcg tccactgatc
     7801 aatcgctgca ccatctgcac tcttcccgtt tcagcattga taaagagcag gaatggagcg
     7861 gtggcgggca gcgcactgaa ttccttcttg gcctgatcga ttgaaagcac tggaacagcc
     7921 tgttgttcca cttcggtggg ctcttcctgt ccccatttaa ctgtgcgccg atctgatacc
     7981 cgcggttttc tggacttact cggcttatga ttcacaagcc gttctttcca ctttctaaac
     8041 tgtgcatcaa cccgatccac aagcgcatca atcgtcgcat acatctctcg agtcgacgtc
     8101 ttcgcctgca cacgtttacc gtttacgttt ccgactacct ctgccttgtg ttgaagcttc
     8161 tcgactccca acacgacttg aagggatccc acctttaacc cgtaccgatc caggcgaccg
     8221 aaccgcgtct ccacatagtc ccgaagcgca ggagtcacat tcatatgtcg accggtaatt
     8281 ctaagtttca taccctcccc cgaatataag ataaatgagg catgacgcga tcacgaggat
     8341 ttagaagaag cgttttcgct ggctggctga cgggatgttc tcttcagctc gatacttagc
     8401 gacggttcgc ctggcgatga gcacttgctg agtgcgaagc cgagcggcta tttcctcatc
     8461 cttcaatggc cgcttcgtat cttcctccgc caccattttc ttgatcattt cccgaaccga
     8521 tacggaggaa tgcatatctg acggctggtc ggcgcgctgc aggcccgcat taaagaagaa
     8581 tttcagttcc agcatgcctt gcggacaata catgtacttg ttggctgtaa cgcggctgat
     8641 cgttgactca tgcataccga tatcctcagc cacctgcttc agcacgaggg gtttgagata
     8701 ctgaacgccg tgctcgaaga attgttcctg aaacttcacg atactcgaca caacctttac
     8761 gatcgtcttg ttgcgctgct caatactccg gataacccac tgagccgccc gcagtttctc
     8821 atccatatag gccttggtct cagcgctccc gccctgcccc gaagccatca gttgcttgta
     8881 gtaggggctg atccgcatgc gcgggagccc atcgtcattc agaagcacca cccactcccc
     8941 ttcattcttg accacaaaca catcaggaac gatgacatag ttctgtgtgt tcgtgaacgg
     9001 ccggcccggc ttgggttcga gattttcgat gactttggtg gcttggaaaa cctcttccac
     9061 cgttacattc agggccttgg caatcttggc gtactgcttc ttttcgaggt ctttcaggtg
     9121 atgaagaata atgccttcaa cgattgatcc cttaagcgct ccgggccgtg accctagtga
     9181 gccgatcgga ttgcggccca aatggcccaa ctgaagcaag agacactcgg gaagatccct
     9241 cgcacctacc ccagtggggt caaatgtctg aatgtcctta aggaccgact cggcctcggc
     9301 ttccgtgaaa tcggttcctg ccaccacctc ggtgagtgca attcggagat acccgtcatc
     9361 gtcgagatta ccaatgatca aacgaccgag tgctttttcg cggtctgaca gtcctgacat
     9421 ggacaattgc cagaggagat gctcctcaag agaagttgcc ttcgccactg tctgctcata
     9481 ggagggaaac tcctcctgtg aagaggaggg atactcggag tcgccggctc gacgatcact
     9541 cccgaaatac tcctcccacc cggaggcagt ggattcttcc ggcgaacccc gttcctcagg
     9601 cgtgccaccc tcgtcaggct ggtcctggct ggtcggagcc ggcgcttctt cagccttttc
     9661 ctcatcgctg gcagtttccg cctcttcggc ttccgcctgt acttcgtcca aaaggggatt
     9721 ttccaggaga tgctgcgcga ggctctgttg cagttccagt cgagatagct gcaacagctt
     9781 gatagcctgc tgcagttgcg gcgtcataat taacttttga ctaagcctca gatccagtcg
     9841 cagtttcatt gtcgacaacc tgctccgcta caacttaaac cggtctccca gatagacagc
     9901 acgggcggtt tcactcttaa cgatggcatc cggcgatccc gactccagga tcagtccttc
     9961 attgatgata taggcccgat ctgtgatcga gagcgtctcc tgcacgttat ggtctgtaat
    10021 caaaatacca atccccttct ctttcaaccg tgtaatgatc tgctgaatgt ccgccacggc
    10081 gatgggatcg atgcctgcaa acggttcgtc taacaacatg aaggacggat ccgtggccaa
    10141 tgcgcgcgtg atctccaatc gacgtcgctc tccccccgaa agggcatagg ccatgcttct
    10201 ccgaatgtgc acgaggtcaa gttccttaag gagcgcgtcc acgcgctgag ttcgttcctc
    10261 acgagcatac cccagcattt ccaggatcgc caagacatta tgctctactg aaagccggcg
    10321 aaagaccgag gattcttgcg gcagataacc gattccccga agggcccgtt tgtacatcgg
    10381 caggctggtc acggattccc cgtcgaaagt aatttctcct tcatccggct ggcaaagccc
    10441 caccatcatg tcgaagatcg tcgtcttacc ggctccgttc gggcccaata gtcctaccac
    10501 ctccccggca cgaacctcaa ttgccacgcc cttgacgacc ttgcggcccc gaaaactctt
    10561 catgagcccg ctggcccgta agcacccaac ccgcttcgag gaggacgatt cggccagcaa
    10621 ctctgaaccg gtgtgaaccc cttgagtcat ggcgcgctct tcccctcccc ctcaattcgt
    10681 acgtgggagc ctccctcgac gacactccga tcttccgcga ggtacatcgt gatctgcttc
    10741 ccgctgacac gcgtcccctt ctcccacgcc accgggtctc ccgtcaggac gatcttttcg
    10801 tcctggctga agtacaccgc cttctgacag gtcgcgttgc cgttttcttt ctcgattttc
    10861 acgtggcccg tcgcctcgac ccgactcacc gatcgattgg acaccgccgg catcgcgtcg
    10921 ctccccttgg gcagggaggg gctcttggtt tggtcgcctc gatcccgccc tcccagagtg
    10981 ggttgcgcgg catcattgtc ctgcgaatga aacatcacca ccattttatc cgagtagacg
    11041 atcagcggcc ccctggttaa caccaccgtt ccctcaaaca ccgcctggct gtctttgttc
    11101 cgcaccgtca tcttcttggc ggtaatggtc gtcgaaacgg caggagcttc tccggtcttc
    11161 tttgccagcc cggactcagc tgaatgcgcg agcggcgcgg ctccgaacac aagagcatta
    11221 agtagcagga gataaatcca catggacatc ctctaaaacc tcaaactcct ccgactgcat
    11281 ccgaccaagt aacccacgtc ccgtcaccac aagcccgtgc ccgacaatcc gcacgggatc
    11341 atccgtacga atttccttcg tctcatcagt ccacaccaga tggttcgtgt aaatcgtgta
    11401 tccgctcacg gtttcaacga ccagcggatc cgcgcgattg gccaatacga aattctttgt
    11461 tccggtgtcg agggttccct cctcaccgga tacggtcagt tccttacctt tcgctccata
    11521 cagtgtgaca tccacatcac tgagcaaagc ccgtttctcc cgttcaaaga gccgtgcctg
    11581 tttggcctgc acctgccact gaatgacatc acctttcgac tgggtgaagg tgaactccga
    11641 aatcctggca tccgcttgtt cgattgatcc cgggggagct gactggctgg tgggtactga
    11701 atccgcattc atgaagagga gatagccaag aaacgccgcc aagatcacac tcaccgcgag
    11761 aagcatccgc ctcgctacaa gttcccacat gcgctagcaa ttctcccaac atacgatcaa
    11821 cagaacgaat cttgcataat cgtagcatgc atggcgggtc aagtaaacgt catcggggat
    11881 ctgaaaacaa aacgactccc acatgtttcc acgtgggagt cgcttcacgg acgtgtcaca
    11941 agcgtcgcgt cttgcttgga cgctatgccg acttttcaga ctctcccgcc ggagactgcg
    12001 ctggcgcggc ggccgacttt ttcttcgtcg ccggttctgc acgcgagacc aactcaatgg
    12061 cgaccatctc tgccgcatcc ccgatccgcc ggcgagtttt cacgattcga gtatagcctc
    12121 ccggacgatc cttgaagcgc ccggcgacat cactgaacag ctttgacacg acggccttgc
    12181 tgcgcaggaa tccgagggcg cgccggcgtg cgggaagcgt cccttccttc ccgaggctga
    12241 tcatgcgatc agtgaatccg cgtatttctt tggcctttgc ctccgtggtt tcgattcgct
    12301 cgtggtccag caaagacgtt acgagactcc gaaacagggc ccatcgatgc ttggtttggc
    12361 gcccgagttg tcgacctttt tttctgtgtc gcacagcgtt ccccttggct caggaattac
    12421 tctgacttcg ggctcccatt gtgaggcgac gttgcatcca ccttcgtgcc cagcgaaagc
    12481 cccatctccg acaagatttc cttgatttca ttgagagact ttttcccgaa attctttgtc
    12541 tttaacatct ccccttccga cttctgcacc agatccgcaa tcgtcttgat gttggcattc
    12601 ttcagacaat tggcggctct gacggacaac tccaactcat tcacactgcg gaaaagattc
    12661 ttgttcactt cccgctgcga ttcctcgtac ccggcctcgc tctttccttc tgcgcgctcc
    12721 tcaggattaa tgaagatatc caaatgctcg cgcaggatgc cggctgcagt cgatagagcg
    12781 tcgcgcggac tgatggtggc atcggtccag atttccatgg tcagcttgtc gtagtcggtc
    12841 atacgaccga cccgcgcatt ttcgacatgg aagttcactc gcttgatcgg ggaaaaaacg
    12901 gagtcgatcg cgatcacgcc gatcggcaac ccttcctctt tgttgcgctc agccgggaca
    12961 tacccgcgcc cgtgcttgat cgtcatttcg atgtccaggg tcgcatcctt atccagcgtg
    13021 gcaatatgca ggtccggcgt gagaatcgtg acatccgcat catgaatgat gtcggatccc
    13081 ttggcctcgc ccggcccttt cttcttcaag cgaatagtct tcggcttgtc cgtatgaagc
    13141 gccaaccgca ggctcttgat gttcaggata atggccgtca catcctccgt gacgcctgga
    13201 attgtcgaga actcgtgcaa caccccttcg attttcaccg tcgtgactgc ggcgcccgtg
    13261 agcgacgaca gcaagacacg ccgaagggcg ttcccgatcg tggtgccaaa cccccgctca
    13321 aacgcctcgg tggtgaacct gccgaatgtt ggggaatgcg catccttgtc gacttccacc
    13381 cgcatcggga cctgaaagtc tttcatcgct ttgatcatga ctcccccttc attaccatcg
    13441 actgactatc acattaggat agagcgtcat gtcctcaaaa acagccccgc tctaccggac
    13501 caatcactat cgagaataca actccaccac catctgttcg ttcaccggca acgtaatctg
    13561 ttccttcgcc ggcaacgagc gcacaatgcc cttgaacgcg cccttatcga gttccagcca
    13621 atcgggaatc ccccggctgt cgacggattc cagcgcggcc tggatcgcag ccaggttccg
    13681 gctccgctca cgcacttcga tcacatcgcc ggccttcacg agcgcaccgg ccacggtgat
    13741 cttcttcccg tttatcgtga gatgaccatg actcacaacc tgacgggcct gtttccgcga
    13801 agccccgaac cccaaacggt acgcgacgtt gtcaagacgg cattcgagta aacgcaacaa
    13861 cgcatcgccc gtaaccccgg tttgccgctc ggcccgctcg aacactccgc ggaactgcct
    13921 ttcttggaga ccgtagatgc gccgcagctt ctgcttttca cgcaactgga ggctatagtc
    13981 tgaagtccgc gggcgaccct gtccgtgctg tcccggtgga tagctccgtc gctcgatggc
    14041 gcacttttcg gtcatgcacc gcgagccttt gagaaatagt ttttcacctt cgcgccggca
    14101 caaccggcag actggaccgc gatacttagc cactgctcta cctccggtta ctggacgcgg
    14161 taattccggc gtccatgtta ctgctgctca caacttactt ccgtccacct agacgcgccg
    14221 ccgcttggga ggccggcatc cattgtgagg aatcggcgtc acatcacgga tcaaattgat
    14281 gcggagccct gccgcctgca acgaccggat ggccgattcg cggccggatc ctggtccgtt
    14341 cacgtagaca tcgatctgcc gcatcccgct ttccatcgcc ttccgggctg cggcttcacc
    14401 ggcacgttga gcggcaaacg gcgtgctctt gcgggatccc ttgaagccct ggttgcctgc
    14461 gctggcccag acgacggtgt tcccgctcaa gtcagtgatc gtcacgatcg tattattgaa
    14521 cgacgcctgg acgtgagcga ctccgctctg aacgatccgc cgttcttttt tcttaccctt
    14581 cttgacgctc atacgaactc cttacttctt tctcagaggg ttatgcgcct ggcgtcgcct
    14641 tcgcggttgg tcttggtttg ctgccgacgc cggaacgccg tccttttctc gtcctggcgt
    14701 tggttttggt ccgttgtccc ctcacgggca gccctttacg atggcgcaaa cctctgtagg
    14761 tgcccgtatc aaccaaccgc ttgatattca gcgagacttc cttgcggagg tcaccctcaa
    14821 cccggtaatc acgctcaatg atttcacgca gcttaacgat cttgtcttca ctcaaatcct
    14881 tgacacggat cgacccgtca accccggcct ctttgagaat ctgcaaagcg gccgcccgtc
    14941 cgatcccata aatgtaagtc aagccgatat cggcccgctt atccctcggc aaatcgacgc
    15001 cagcaatacg tgccatgatg tcctgttaat cctttcttct ctgatcgcgg gccactaccc
    15061 cgaccagccc cacaaccatg atcagccctg gcgctgcttg tggcgagggt tatcgcacag
    15121 aattcgaacc acccctcgtc gacgaacgac cttgcatttc gcgcaaattg gctttacgga
    15181 tgatttgact ttcatatgga ctccaatgct cccgctactt gaaacgatag gtaatacggc
    15241 ctctggtcaa atcgtagggc gacatttcca ccgttacctt gtcgccggga agaatacgaa
    15301 tgaagtgcat ccgcattttt cctgaaatgt gtgccaaaat cacgtggccg ttatcgagct
    15361 tcacccggaa cattgcattg ggcaatgttt ccgcaaccga accttgtact tcgattatgt
    15421 cttctttcgc cacgtactcc tgttcctctt ccctgtcgac ttaccctgca cgctctgaac
    15481 gctgactcag aacgcgggcc ggaccattcg gctgtatcgc aatcgtatgt tcgaagtggg
    15541 cggagagact tccatctgcc gtcaccgccg tccaccgatc ctcaagaact cgcacggcac
    15601 tcccgcccat attcaccatg ggctctattg ccagcaccat tcccggctgc aatcgtggtc
    15661 cctgtccagg cttcccgtag ttgggaacct gcggctcttc gtgcagctgc cggccgattc
    15721 cgtgcccgac aaattccgtg acgaccgagt accccgcgga ttcgacgtaa cgttgaacgg
    15781 catgcgaaat atccgtcaac cgattgccga ccactgcctg ctgaatcccc aggtagagcg
    15841 cttcttccgt tacttgaacc agtttggcgg tcgcctcgga aatccgaccg acagccaccg
    15901 tcactgccga gtcaccatag aacccgccga cgatggcacc gagatccagg ccgatgatat
    15961 ccccgtcctt gagcttccgc tttgacggga tcccatgaac gacctgctcg ttcaccgatg
    16021 cacaaagggt tttcggataa ctccggtacc ccttgaatgc cggggttccc cctctggccc
    16081 gaatcgcctc ttcggcgatc cgatccaagt catcggtgct gatgcctgga cgcacttcct
    16141 tccgaacgat ctccagtgcc tcagccacca ctctcgacgc ctctgccatc acttcgattt
    16201 cagcaggcgt tttcagaatg atcatgccgt cttgtacgcc accagcacct tcgtcaggcc
    16261 ctgatacacc gcctcaacgg atcccgcacc atcgagatgt gacaacatat ttttcttctc
    16321 gtaaaagctg atcaacgggg ccgtctgttc ttcatacact ctcagccgcg tctcgatcgc
    16381 ctcccgttga tcatcgcttc gctgaatcaa tgcttcgccg caccggtcgc acaggccgcc
    16441 cttcttcgaa ggagcaaaat ccacatgata tgtggcctgg catttcggac agcttcgtcg
    16501 cccgctcagt cgtttcacaa cgtcctcgcg agagacttga aaattaatca cgcggtcgag
    16561 gcgaaatcct cgttcgttca gcactttccc aagttcttct gcttggggaa ccgtcctagg
    16621 aaatccatca aggataaatc ccttcgcata acttggatcc gccagcttgt cgcgcaccag
    16681 accgatgact accgaatcag gaacgagctt cccctgatcc atatacccct tggcctcgag
    16741 gccaagcttg gtttgattgc ggaccgcttc tcgaagcaaa tctcccgttg aaatcttcag
    16801 gacaccgtac tgagccgcaa ccttgtccgc ctgcgtgccc ttaccaacgc cgggagcacc
    16861 gagaaacacg agctgcataa accgttatcc gcttcttccg cgcaacgggg ccatgccttt
    16921 gcccaggaat ccctcgtagt tgcgcatcaa catatgcgat tcaatctgct gtgcggtgtc
    16981 tagccctacg ccgatcacaa tcaacaaaga agttcccccg aaatagaacg gaacattcag
    17041 cttgtaaatc aagaactccg ggatgacgca cacaatggcc aggtaaatcg cacccgcaaa
    17101 cgtaatcttc gtcaggacat tatagatata gtccgaggtc cgttgtccgg gccggatgcc
    17161 cgggataaac ccgccgtact tcttcatatt gtcggccata tccacagggt tgaggacaac
    17221 cgccgtgtaa aagaaacaga aaaagacgat caatccgaca tacatcaatg tatagagcaa
    17281 cgaccctggg gccagctgcg tcccgatcgc cttcacccat ggcgtttcaa aaaaccctgc
    17341 gatggtggca ggaaacgcaa taatggatga ggcgaaaatc ggagggatga cacccgccgt
    17401 gttgatcttc aatggaatgt gcgtactttg tcctccatac acacgacgac cgataactcg
    17461 cttggcatat tgcacgggaa tcttccggcg cccgctctcc aaaaatacga tggccgcaac
    17521 tacagcaacc atcagcgccg ccagggcgac cagcaataca atgttgagct gtccgacctt
    17581 gtagagatcg aaggtttgag ccaccgccgc cggcaatcgc gccacgatcc cggcaaaaat
    17641 gatcaatgaa atcccgttgc cgattcctcg ctcggtaatt tgctcaccca gccacatcag
    17701 gaagcccgtg cccgccgtca gcgtgatcac ggtcatgagg cgaaaccccc aacctgcagt
    17761 gaggacgaac gcgccctgat tcatctgctc gagcccgatc gcaatgccga atccctgaat
    17821 cagcgcaatc gcaatcgtcc cgaagcgcgt atactgaata atcttcttcc gtccccgctc
    17881 gccttccttc gccaacttcg acaaatgcgg aacgacgacg gtcaacagtt gaagaataat
    17941 cgatgcgctg atatacggca tgatgcccag cgcaaagatc gtcaagcggg acagcgagcc
    18001 gccagagaaa atgtccagaa acccgagaag cgcgccgccc tgcttctgca aaaactcgga
    18061 aagggcctcc ccgttgattc cgggagtcgg gatatgcgcc ccgacacgat acaccaccag
    18121 catcccgagc gtgaacagca cgcgagtacg aagctcgggg atcttgaaga tattctgaaa
    18181 actggtcagg agtcgctcaa acaccgccga taacctcgac tctccctcca gccgcttgaa
    18241 tcttcgcctc tgcagacttg ctgaacttat gagcttgaat gacgagggac ttcttcaact
    18301 cgccgttccc gagaatcttg atcggcagct ttttccgctt gaccaggccc gcgtcgacca
    18361 tcgcctgcgg agtgatggtc tcgacccccg tccacttttc aaagctcttg acgttgacga
    18421 tggcgtactc ggtccggaag ggattggtga aaccgaattt cgggagccgg cgaaccaacg
    18481 gcatctggcc gccttcaaag ccgggccgct tgccaccgcc ggatcgagct aacaaaccct
    18541 tgtgcccctt ggtcgcagtc ttcccgtgac cggaacccgg tccacggccg atacgcttcc
    18601 gtcgcttctt tgcgcctctc gctggagtta aatcatggag attcatggct tcccaacctc
    18661 aagcagatag cccaccttat gaatcatccc gcggacctgg ggcgtgtccg gatggacggc
    18721 ggtctggcgg atgtgtcgga ggccaagacc acgtaataca agacgatgcc gttccggagt
    18781 cccgatcggg ctgcgcttca gcgtcacacg aacaccctgg gtccccgact tgctggtttt
    18841 ggatggcttt gctgtgccca tcagaccgcc gccctttcat gccgctccgc tgccgtctgt
    18901 cggcggagcc gaagtacttc atcaggattt ctcaattgcg tgagaccatc gagcgtcgcg
    18961 cgaaccgtgt tgaaaggatt gcctcggcca agcgtcttcg caatcacatt atgcgcgccg
    19021 accaattcga ccaccgcgcg aaccgctccg ccggcgataa ttcctgtccc gtcgacggcc
    19081 ggcttgagca atacatgctc gccgccgaag aggccatgca cttcatgcgg gatcgacccc
    19141 cccttgagcg ggacgtgcac gaggtgcttc ttggcctgtt caacggcctt tgaaatggca
    19201 acggggactt ctgcggcttt ccctttgcca atcccgaccc acccatggcc atctcccacg
    19261 acaacgagtg cgcaaaagtt aaaccgcttg ccgcccttta ccactttggc cacgcggttg
    19321 atgaagacga ctttgtcttt caggcttagt tcatcaggat tgactcgcac gcagtgcctc
    19381 tcttcttccc tacccactca gagtcagtgg ggcctaatag gattaactgg gctaaaactg
    19441 aagtccccct tcacgggacg cgtcagcgag agccttgatt cgaccgtgat acatccgccc
    19501 cccgcgatca aagaccacgg ccgtcacctt ggcggccttc gctcgatcag cgatcaactt
    19561 tcccaccgct tttgccgcct caatactgcc ggtcgacttg agggacttcc gcaacgactt
    19621 gtcgagcgag gatgcggccg caagtgtggt tcccttgatg tcgtcgatga tctgagcata
    19681 gatgtgggaa ctgctccgaa acacattcag acggggacgt tccgccgttc ccatgatcgt
    19741 gtgacgcacc cgttggcgcc gtcgttcaag ctgtcgtact ttatcagcag tattcatggt
    19801 cagttcctac ttcccggtct tgccttcttt tttccgcaac acttcaccca tgtagcggac
    19861 acctttttgc ttatacacgt cgggcggctt gatcgcccgc agattcgcgg cgacctgacc
    19921 gaccaagcgt ttatcggccc ccttaatatt aatgagggtc tgcttgtcca ccttcacatc
    19981 gattcccgtc ggaacctgat agacgacggg attgatgtag ccgacgttga agctcatcgt
    20041 ccggccctgg atctgtgtct tatagccgac tccggtaatt tccagagatc gctcgtagcc
    20101 cttcgtgaca ccatggatca tattgtttag ttccgcgcgg acaagcccgt gcagcgcacg
    20161 cagttttcga tcatcactgg aacggctcac gacaacctga ccgttatcaa ccgcaacgcc
    20221 caccccttgc gcgagcgacc aatccaattt tcccaaggga cctttgatcg acacggtcga
    20281 cccggacact ttgacttcca ctccacctgg aatcgctatc ggctttttcc caatccgtga
    20341 catcgtcttc tacgcctcta cgctagtcgt gaatgcccac accgcctacc aaaccgagca
    20401 aagaatctcc ccgcccaatt tattccggcg cgattcctga tcggtcatga tgcctttcga
    20461 cgttgaaagg atggacatcc cgatcccgtt ccggaccttc gtgatgtccc tacttcccac
    20521 atagacgcga cgcccaggct tgctgatccg ctccaatccc gtaatcatgg gacggccttc
    20581 atccacatat cgcaagtgca cattcagaac aggatgcccg tcacgcactc cgtcctcgac
    20641 cgactcgacg tagccttcct tcgtcaaaat ttcaagaatc gcgcgcttca attttgaagc
    20701 cggaacggca atcgtctcat gccggcgttg agcgccattc ttcaaccgaa caagaagatc
    20761 gctgattgga tcagttacca tgctgtatcc ttctaccttc ctaagacaat ctgccccgaa
    20821 acgacacgct ctctaccagc tcgatttccg gacgccgggg atttctccgc ggaggcttag
    20881 aaaccgaaaa caaattctgc acatccgaaa gcggcgcaga tatccccgca cgcggccgca
    20941 cacgccacag cggtgatatt cccgagagga aaatttctgc ttggttaccg ctttgtttct
    21001 caacgctaat cgtgacacaa ccactccttc cgttcttcag tccccacggg acgccggcgc
    21061 ccctacgctc gaaacggcat ccccagatgc ttcaagaggg ctttcccctc gtcgttcgtc
    21121 cgggccgtcg tcacgatcgt aatgtccatt ccgtgaatgg aggcgacttc gtcgtacttg
    21181 atctcaggga aaatcaattg ctccttcaaa ccgagcgtat aattgccacg accgtcaaat
    21241 gccttcggag acaccccgcg gaaatcacga atacggggaa gcgccagcgt cacgagccga
    21301 tcgaaaaatt cgtacatccg gcggcttcgg agcgtcactt tggtaccgat cgggaggccc
    21361 tgccgcagct tgaatccggc gatggccttc ttggcacggg tgaccacggg cttttgcccg
    21421 gtgatcgtcc ccagttcggt taccgcactc tccagcaact ttacgttctg aatcgcttct
    21481 cccatcccga cgttcaacac gacgcgctcg agcttgggca cctgcatgac attcttatag
    21541 ccgaactctt tcatgagcgc cggcaccacc tgctgcagat acatgtcgcg gaggcgcggc
    21601 cgaaacttcg actcttcgct tccttgatcc agct
//
LOCUS       DATIFR010000017        24038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_392726, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000017 DATIFR010000000
VERSION     DATIFR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24038
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392726"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VLD60_00625"
     CDS             complement(<1..203)
                     /locus_tag="VLD60_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSB43480.1"
                     /translation="MAKSLIIVESPTKAKTITKYLGRGYTVMASVGHIKDLPTSKLGV
                     DLEHDFKPQYVTIKGKSKVLADI"
     gene            complement(292..1407)
                     /gene="dprA"
                     /locus_tag="VLD60_00630"
     CDS             complement(292..1407)
                     /gene="dprA"
                     /locus_tag="VLD60_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSB43481.1"
                     /translation="MNATLEAWLKLRAIEGVGDQTVLALVREWQRPEAVLQASNDELV
                     QRGCSSRLARAITRGPDPDACRRIDRELAMFQRARIDARTILDPSYPARLRMIPDPPP
                     LLYVSGTLTEQDELALAIVGARRATAAGRVLTEELAKDLATAGFTVISGLARGVDAAA
                     HRGALAAGGRTVAVLGCGIDRTYPIEHERLRREIEEQGAVVSELSCGSPPHSAHFPRR
                     NRIISGLSLGVVVTEAAINSGSLITARLAAEQGREVFAVPGFVKEETSHGTNALLKEG
                     ASLIEKAHDVIEAVLPQLDVSLRARLSCMPAEMKRGDRFGNEEQLVYDALSYDPLTVD
                     HLVAQTGLPVPLVAASLLSLELRQRVRQLSGQRYLRV"
     gene            complement(1509..3017)
                     /locus_tag="VLD60_00635"
     CDS             complement(1509..3017)
                     /locus_tag="VLD60_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HSB43482.1"
                     /translation="MGVEIAITVAREETRVAVLDGGVVTDLFGDRAKHKDFVGNIYKG
                     KVAKVLPGMQAAFVDIGLEKAAFMHVSDLSMDAEPGDTLVDSDDDDKDGDELRPRRES
                     SKPIEQLLSEGQELMVQISKGPIGTKGPRVTTYVSLPGRYLVFMPNVEHIGVSRRIAR
                     DEERARLKDIMKRVRHPGCGYIVRTVSEGVKEDELRSDVDFLHVLWQDIRTKRDQQAA
                     PALLHTDLSLSFRVVRDLFGKKVERLWIDSRQEYEAVRDFVQRFSPEQTSRIHFYDKD
                     ESLFEYLGVEQEMARALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETIL
                     RNNLEAAKEVAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMASDKARTRISR
                     ISDLGLIEISRERVREDLLRSLSEPCRYCEGRGYTKSPTTVAYEIFREIRKIGSGNEQ
                     QRIVVGAHPSVAGLLQDEERQGLEALERECSAKVIVMPDEQLHLEQYDLAVL"
     gene            complement(3181..4302)
                     /gene="rodA"
                     /locus_tag="VLD60_00640"
     CDS             complement(3181..4302)
                     /gene="rodA"
                     /locus_tag="VLD60_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSB43483.1"
                     /translation="MINRVMDRRGLDSFDLRYLGLIFVILGIGVLSIYSVTHDQGGPG
                     LPFYAKQIVWILLGIVAFLIMLVWDYHRIARLAYPAYAIILILLAVVLFEGKTSRGAQ
                     RWIPIGPFAFQPSEFAKLVLILVMAHYYSKAPRVGWLQRVVIPGALMLPGLLLILKQP
                     DLGSGLSFMAVYAAMLLMVGMRSQALGVILLFSLMLFPFAWEIMWGSLHDYQRQRIMA
                     FVDPTYDPGGKGYHALQSRIAIGAGELTGKGLYGGTQSQLKFLPEGHTDFVFSVFAEE
                     WGFIGVLVLLLLFVALIWLSLEIATRAKDELGALLAVGVISMLCFCVVVNIGMTAGMF
                     PIVGIPLPLMSYGGSATIMTMASLGLLLNVKRRRLSLFY"
     gene            complement(4299..6173)
                     /gene="mrdA"
                     /locus_tag="VLD60_00645"
     CDS             complement(4299..6173)
                     /gene="mrdA"
                     /locus_tag="VLD60_00645"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSB43484.1"
                     /translation="MAIARLHDSELGELQRRLVVLRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVILEPARGLIYDRNGVLLANNVPSFSLYVSLEDVKDREALIETL
                     TELIGLDPALVRKKLAVRGAKQLPRKIKDRLTLREATLIESHRLDLPGVMIQVESQRN
                     YPSGVTASHVLGYVGEISAEQLDKPESAELHQGSIVGQYGVEKFFDHIVRGQAGQKSI
                     EVDALGHEKRTVLVDQPQAGDDLYLTIDARLQKVAEDLLGEESGAIVALDPTTGDLLA
                     MASRPAFDPNVLSRELTSKQWVEIVQDEGRPLNNRASQGQYPPGSTFKVLMAAAALES
                     KTMTPYSTVFCTGGYQFGKRVYHDWKASGHGSVDLRKALIHSCDVYFYTVGQRMGIDT
                     MASYAHQFGLGEETGVELPSERIGTVPSTAWKQKVKHEAWLPGETISAAIGQGYVTVT
                     PLQMASLIGTVANDGVTYRPRLVQAIMDRETGQRQEIPAVPKSTLDVKPDTLKLIREA
                     LAGVVTEGTATRAKSSIVTIAGKTGTAQTAALRTGPEKDIPKKFRDHAWFVAFAPVEN
                     PTIAVAVLAEHMGHGGSAAAPLAKEVIETYVKLKSNAPKGSTEAATSAPTQTMEGRAL
                     "
     gene            complement(6195..6698)
                     /locus_tag="VLD60_00650"
     CDS             complement(6195..6698)
                     /locus_tag="VLD60_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43485.1"
                     /translation="MKVLLYLGLVLLLVPVQTTLLPHVSLWGIKPDLGLVIAAVIGLI
                     AGELEGLLVGLAIGWVLNLYSAGDLWLSIVTTGGAGLFAGLLGRQVAQVTPTILCIGL
                     LTLSLAGGVVAVFSLKNATMSETWWMIQSVVLPQACFDALLGAGVLWLMEQRFAVQRL
                     RTFGRFS"
     gene            complement(6695..7570)
                     /gene="mreC"
                     /locus_tag="VLD60_00655"
     CDS             complement(6695..7570)
                     /gene="mreC"
                     /locus_tag="VLD60_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSB43486.1"
                     /translation="MVNVRSPYGARRLALGLFAFILAALFLFPSQSQGLLDFLDGPVG
                     QLVRLPLEAVASLDQGISDVWQRYVTLQGVEDENRRLRQDIEWLRGENNHLRESASAT
                     QRLTTLLRFKEQALPTMVAAQVIGKDATNRYRSVILNKGESDGIHPDMGVVTPSGVVG
                     RVVKTTGGTSVVLLVTDPNNAIAGLIQRTRDEGIVEGTPQGKVRLKYIPLLSGVRDGD
                     RVVTSGLVGGFPRGLAIGTITGIAKEEGALFQTAELTPEVDVNRVEEVLVIQSPQGQS
                     AAVEEKTDSAAKSRP"
     gene            complement(7595..8644)
                     /locus_tag="VLD60_00660"
     CDS             complement(7595..8644)
                     /locus_tag="VLD60_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSB43487.1"
                     /translation="MGFTSDIFGWFSNDLAIDLGTATTLVYVHGKGIVLNEPSVVAVE
                     KKTEKVLAVGAEAKKMLGRTPGNIVAVRPMKEGVIADFEMAEQMLKRFIQKAHNRSAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDVGGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEIREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLAKVAVMSQANTFR"
     gene            complement(8690..9157)
                     /locus_tag="VLD60_00665"
     CDS             complement(8690..9157)
                     /locus_tag="VLD60_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSB43488.1"
                     /translation="MAEQVGVHPLGIGIYPKAQVLNRFIAKLIDLFLAAAAAEVIAPV
                     GFLAGLAYLLVADGFAGGRSVGKRLIGLQTIRPGTRETAGFRESIIRNIPFAVAQLAF
                     AIPYVGPYVGWLVSAVIVAFEAILIIGNEQGRRLGDELAGTQVLDAGRLAMPD"
     gene            9348..10046
                     /locus_tag="VLD60_00670"
     CDS             9348..10046
                     /locus_tag="VLD60_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSB43489.1"
                     /translation="MIKILRSANEKYPWFLALTMGFIAISFIIGMGWWGFGEHQGTVV
                     ASVGDLTISHDEFKRAYENTYRFYKDKVPGEFKDETIKQYVVEQLIDNRVWLIAAKNM
                     GLTVTDEDLRDVIMQIPEFQRNGTFDPELYQRLLAANHLTPAIFEAMEAKELLSNRAR
                     MMISDAVTLTPSELAEAQALTLRQTEPDPAKAASAKDRAIQDVLFQKQQRALMAYTQS
                     LKATVPINIRREML"
     gene            complement(10141..11172)
                     /gene="queA"
                     /locus_tag="VLD60_00675"
     CDS             complement(10141..11172)
                     /gene="queA"
                     /locus_tag="VLD60_00675"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSB43490.1"
                     /translation="MQLSEFNFPFDPALIATEPVLPRDQARLLVLNPTSQSLSHRKVA
                     DLPDLLTPGDLLVVNDTKVRAARVMGRKRISGRPVEILFVKEVAERSWEVLMKGKWRA
                     GQQIDADGGMCLTVLSRDAGRTVVRIEGPLSTHEFFRIHGRMPLPPYIKRTPNDRDRD
                     WYQTIFAHDEGAIAAPTAGLHFTRALLARLADRKIGLARVTLHVGIGTFKPVTVDRIE
                     DHRMGAEWVDVGMETVRAIERTKQSGGRIVAVGTTVVRALETAAQVQGEVRSFQGETR
                     LFITPGFAFHAVDALLTNFHLPQTTLLMLVAAFAGVDLLRKAYEEAVRGRYRFYSYGD
                     AMLISHRRP"
     gene            complement(11172..12386)
                     /locus_tag="VLD60_00680"
     CDS             complement(11172..12386)
                     /locus_tag="VLD60_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSB43491.1"
                     /translation="MNSRFYRVRAGLIVCFLLTLAASWAEGAQSIRVLLAADVHKMDI
                     QAEGAVWVADSSRQGRAIHSTVRVIPYGKGFLVNGVRMAHERLTLSGGEQGLTLLLAK
                     PNGKAHGTSKPANESRLALPVSGLVHVVRRGDGMLVINQVDLEEYVKGVVPAEVNSTW
                     HPEMLKAQAVAARTYALYQQMLSATRDYDVAATVQDQVYRGKNGIDAAVLRAVDETRG
                     LVLTYQNAPIYAAFSSTAAGLTEDAVNVWSKEYPYLKGVECPFDLESPYYQWKVSFKL
                     DALEQNLRRQGFAVGTIASITPLAFSRGGRVAKLLVVHSGGELVLRGEDFRKAVGYSI
                     VPSTQFTIDSVGQEILLSGYGAGHAVGMCQWGAKELAELGYPFSTILEYYYPGTDLKS
                     MALTKQPVPPSF"
     gene            complement(12383..12637)
                     /locus_tag="VLD60_00685"
     CDS             complement(12383..12637)
                     /locus_tag="VLD60_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSB43492.1"
                     /translation="MVEWASIGKLFIGIGAVMAVLGVLFLLADRVPGIGSLFGWFGKL
                     PGDISIKRDNFSLFFPLGTSLLLSVVLSLLFYLVSWIFRR"
     gene            complement(12637..13659)
                     /locus_tag="VLD60_00690"
     CDS             complement(12637..13659)
                     /locus_tag="VLD60_00690"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSB43493.1"
                     /translation="MLKKKSAYVVAILGATGAVGKETLEILEEREFPLASLRLFASKR
                     SAGEVMSCQGKEWKVEELTPESSFVGVDFALISATDAISRDYGQRLGTAGVVVIDDSG
                     VFRMEDDVPLVVPEVNAHALTRMPRGIVSIPNCTTTPLVMALKPLQDAVGVKRVVVTT
                     FQSVSGTGAAAMDELMTQTKDLMAFRDAKAEVYPYQIAFNLLPHIGSFTEGGDCSEEV
                     KIARETRKILEAPALRVTATTVRVPVLRCHSEAINVELERPLKSNEARAALAAMPGVI
                     VYDDPSKKLYPMPLDATGKDEVYVGRIREDESIANGLNLWVVSDNLRKGAALNAVQIA
                     ECLVKG"
     gene            complement(13674..14879)
                     /locus_tag="VLD60_00695"
     CDS             complement(13674..14879)
                     /locus_tag="VLD60_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43494.1"
                     /translation="MSTGVKMGTIRTVAGTGESGFLGDGGPATAACLNEPKAVTVDGR
                     GNLIIADSENHLVRAVNRETGQIRTIAGYVGVPEPRLEVVKAHSVPEPAGDDPFSDAG
                     TTGERAFAQQTDLSGTVRYVVNGAGTARRFGGDGGRADRALLNFPTAVAVDKAGNLYI
                     ADTMNHRIRRVEAGTGLITSIAGVGQPRYGGDGGPAVAAGLNEPAALAVSESGTLYIA
                     DQSNNRVRAIDLSTGLIRTVAGIGLASYNGDGIPATEAALAGPSGLALGKDGTLFIAD
                     TFNGRIRAVDAATGMINTVAGDGGDYRYQGAVEPPSSSLSRPSGIAVDTEGNILLTDS
                     DNHLIRRWNRSTGRLDRLAGVGVANYGGDGGTALEASVNYPFGVAVDCDGHLFVADTF
                     NHRIREIVL"
     gene            complement(14897..15970)
                     /gene="leuB"
                     /locus_tag="VLD60_00700"
     CDS             complement(14897..15970)
                     /gene="leuB"
                     /locus_tag="VLD60_00700"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HSB43495.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLKVVAEKFGHQFEFAAGDVG
                     GQAIDKVGVPLPQDTLTLAKQSDAVLLGAVGGPKWEGLEYSLRPERALLGLREQLGLY
                     ANLRPAKLYDVLADASTLKKEVIAGIDILVVRELTGGIYFGKPKGIEKMPNGSERGIN
                     TEVYTTEEIRRIAKVAFEAARKRRKKVTSVDKANVLESSELWRRVVIEVQKDYPDVEL
                     NHIYVDNAAMQLVRNPRQFDVMLCNNMFGDILSDEAAMLTGSIGMLPSASVGAAVGLY
                     EPIHGSAPDIAGKNIANPIATIASAAMMLSYAFKLDKEADVIEQAIVKTLDLGYRTKD
                     IQGPSTRLVGTTEMGDAIIRNLN"
     gene            complement(15987..16364)
                     /locus_tag="VLD60_00705"
     CDS             complement(15987..16364)
                     /locus_tag="VLD60_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB43496.1"
                     /translation="MAGAPVFKVTPSDRPEFLAGDHTRLREILHPAKHQLNLGYSLAH
                     GTLGPGRRSKWHVLASSEVYYFIAGQGRFMIDDQVLSVEPGTTLYVPPGGKQSLENTG
                     NSEIEFLCVVEPAWKVEDEQVLE"
     gene            complement(16414..17961)
                     /locus_tag="VLD60_00710"
     CDS             complement(16414..17961)
                     /locus_tag="VLD60_00710"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSB43497.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKVMVAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIAQEVEGPTICSLARARPEDIDRAWEALKGAPKVRIHTFLST
                     SDIHLKHQFRMTRDEAKKRAVEMVQRARGYVEDVEFSPMDASRSDPAYLYEVIEAVIA
                     AGAGTVNIPDTVGYAIPQEFGKLIRGIREHVPNSSKAVISVHCHNDLGLAVANSLAAV
                     MEGAGQVECTINGIGERAGNTSLEEIVMGLRTRRDLYQADTKIHTEEISKTSRLVSKI
                     TGMVVQPNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESIGLVESKMVMGKLSGR
                     HAFRQRLEELGYKLTDEEVNHAFERFKKLADHKKEIYEEDLEVIVSEELAKMADRITL
                     KSFHVASGTDQVPTATVELEIDGKSITQTGTGDGPVDAVYRTIAAITQTKSKLLMYVV
                     KAITGGTDAQGEVSVRVQEDGRTVSGHGADTDIITASARAYLSALNKLAYLAAKQAQG
                     EQRVNLI"
     misc_binding    complement(18013..18248)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18245..19051)
                     /gene="pssA"
                     /locus_tag="VLD60_00715"
     CDS             complement(18245..19051)
                     /gene="pssA"
                     /locus_tag="VLD60_00715"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HSB43498.1"
                     /translation="MKANGLRQSFAKGGKRHKAAMHLIPNLFTTGNLFCGVFAILSVF
                     NANYLAAAVAILVAMVFDVLDGKSARLTNSTSHFGLEYDSLSDVVSFGVAPGLLIYSW
                     ALSGQGTFGIAVMFAYVAMGAVRLARFNSTVAVSDGKYFTGLAIPAAAGVVASMVIFD
                     HHIVRTGAEVKPIVVLIVTLVLSFLMVSTIKYRSFKDMKFRGGQHITYLVWGILGLVL
                     VAAWPQVMLFVIFAGYALLGPFERMLGMFAKLAGKQPQLKREQPAVDQKS"
     gene            complement(19048..19698)
                     /locus_tag="VLD60_00720"
     CDS             complement(19048..19698)
                     /locus_tag="VLD60_00720"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HSB43499.1"
                     /translation="MADRAVGVPFAKEGFPFIGAAAGVTLLVGWLGWTVAAVGAALLT
                     LFVSWFFRNPARVIPQGAALVVAPGDGKVIAIEEEFEPRYLKDRSIKLSIFLNVFDVH
                     INRIPCEGLVEDVQYQPGLFMVASKPEATIQNEQNAVMIKTVQGAKVLCVQVAGLIAR
                     RIVCWVSPKEKAVRGERFGLIRFGSRMDTFLPIGTVIKVAVGDHVKGGETILGELR"
     gene            complement(19713..20726)
                     /gene="ilvC"
                     /locus_tag="VLD60_00725"
     CDS             complement(19713..20726)
                     /gene="ilvC"
                     /locus_tag="VLD60_00725"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSB43500.1"
                     /translation="MKIYYDKDADIQQIKNKKVAVIGYGSQGHAHALNMKESGVSVVI
                     GLREGSSWRKAEQSGLKVMPVADAVKASDVIMILAPDEAQASIYRQEIAPNLKPGSYL
                     AFGHGFNIHFGQIVPPSTINVFMVAPKGPGHLVRSEYTKGSGVPCLLAIHQDPSGTTK
                     QVGLAYASAIGGGRAGVIETNFREETETDLFGEQAVLCGGLTSLIQAGYETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTEQTKQEMKKI
                     LAEIQQGQFAKEWVLENQANRPVYNALLAKGEAHPIEEVGARLRAMMPWLKKDQLVDK
                     TKN"
     gene            complement(20788..21306)
                     /gene="ilvN"
                     /locus_tag="VLD60_00730"
     CDS             complement(20788..21306)
                     /gene="ilvN"
                     /locus_tag="VLD60_00730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSB43501.1"
                     /translation="MEHIISVTVENKFGVLSRIAGLFSGRGFNIESLSVAPTLDPSMS
                     QMIIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESDFVSRETALIKVHTRQEDRAEAL
                     RIADIFRANVIDSTPATYTIEVTGDPKKIEAIINLLQPLGIKELTRTGRVAVAREPLR
                     SSSAQPKKVARE"
     gene            complement(21330..23105)
                     /gene="ilvB"
                     /locus_tag="VLD60_00735"
     CDS             complement(21330..23105)
                     /gene="ilvB"
                     /locus_tag="VLD60_00735"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSB43502.1"
                     /translation="MKLTGAEIFIECLKREGVKTIFALPGGVVLKIFDTLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCFSGQV
                     PTSLIGNDAFQEADNVGLSRPCTKYNFLVKDVNDLASTIKEAFYIATTGRPGPVLVDI
                     PKDVSMEKAEFNYPNSVSIRGYNPTYDGNKWQIKQAAEAIMKAKKPILYVGGGVVFSG
                     ASQELIELAELTQIPVDMTLMGLGAFPGEHPLSMGMMGMHGTYQANMAVHYADLVVAI
                     GARFDDRVTGKVSEFCPYAKIVHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILKA
                     TVNGEQKDLRKAWWDQIREWERTHPLTYQQEKDGPIKPQHVIKRLYELTNDRDPIVST
                     DVGQHQMWAAQYFKLAKPNRWLTSGGLGTMGFGFPAAMGAQAAFRNRLVLCIAGDGSV
                     QMNMQEMATAVVHKLPVKIIILNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAE
                     AYGAVGLRAGKVGDLDGILKEAIAIGGPVIVDVPTYPYENVYPMIPAGGCNHEMILED
                     PPELKNKQAGAGKTAPKDSDTILTA"
     gene            complement(23578..24005)
                     /gene="rnpB"
                     /locus_tag="VLD60_00740"
     ncRNA           complement(23578..24005)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLD60_00740"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         5161 a         7657 c         6065 g         5155 t
ORIGIN      
        1 ttgatgtcag ccagaacctt cgatttcccc ttgatggtga catattgcgg cttgaaatca
       61 tgttcgagat cgacgccgag cttgctggtg ggtaaatcct ttatatggcc gaccgaggcc
      121 atgaccgtat aaccacgccc aagatacttg gtgatggtct tcgccttcgt gggtgactcg
      181 acaataatta acgacttcgc catatcgact ccaatgccta cgacacctta ccctatcatc
      241 cgtaccaaat atagcagatc agtgcaactc gcctggtcct tgagcgaacg atcacaccct
      301 gagatagcgt tgtcccgaaa gttgtcgcac ccgctgtcga agctcaagcg acaagagcga
      361 cgccgccacc agcggcaccg gcaacccagt ttgcgcgaca agatgatcca ccgtcagcgg
      421 atcgtacgag agggcatcat acaccagctg ttcttcattt ccaaaacgat cgccgcgttt
      481 catctccgcc ggcatgcatg aaagccgcgc gcgcaacgag acgtcgagtt gcgggagcac
      541 cgcttcaatc acgtcgtggg ccttctcgat caacgaagca ccttccttca gcaacgcatt
      601 cgttccatgg ctcgtctctt ccttcacaaa acccggcacg gcgaagactt ctcttccctg
      661 ctccgcggcc aagcgagcgg tgatcaacga tccgctgttg atcgcggctt cggtcacgac
      721 gacacccaga gaaagtccgc tgatgatacg atttctccga ggaaaatgtg cgctgtgcgg
      781 aggcgatcca caagacaact cggatacaac cgccccctgc tcttcgatct cacggcgaag
      841 ccgttcatgt tctatcggat acgtccgatc gatcccgcac ccgagcaccg ccaccgtacg
      901 acctccggca gccagtgccc ctcgatgggc cgctgcgtca acgccgcgcg cgagcccgct
      961 gatcacggta aacccagcag tggctaggtc cttcgctagt tcctccgtca ggaccctccc
     1021 ggccgcagtc gcgcgcctcg caccgacaat ggccaaggcc agctcgtcct gttcggtcaa
     1081 tgttcccgat acgtagagaa gcggcggtgg atccggaatc atccgaagac gggccggata
     1141 agaagggtca agaatggtcc gggcgtcgat cctggctcgc tggaacatgg ccagttcccg
     1201 atcgatcctc cggcaggcat cgggatcggg accgcgtgtg atcgcccgag ccaaacgcga
     1261 actgcaacct cgctggacga gctcgtcgtt cgatgcttgc agaacggctt ctggcctttg
     1321 ccattcacgc accaacgcca agaccgtctg atcgccgacc ccttcgatgg ctcgaagctt
     1381 caaccaggct tcgagtgtcg cgttcactgt ccttctttct gcaaggcggc tcaccattgc
     1441 tccctgccgg cgatcagcaa gacgacgctg gaatcattgg cccaagctgg ttccatactc
     1501 caacatccct acagcaccgc aagatcatac tgttccagat gtagttgttc atccggcatc
     1561 acgatgactt tcgcggaaca ctcccgttca agagcctcga gaccctgacg ttcctcgtct
     1621 tggagtaggc ccgccaccga gggatgcgca cccaccacga tccgctgctg ttcgttgccg
     1681 ctaccgattt tccgaatctc gcggaagatt tcgtacgcca ccgttgtcgg cgacttcgtg
     1741 tatccccggc cttcgcagta ccgacaaggc tccgagaggg atcgcaacaa gtcctcccgc
     1801 acccgctcgc gggagatttc gatcaggccc agatccgaga ttctcgaaat tcttgtccgt
     1861 gccttgtcgg atgccatcgc gtcgaccaag gcatggtata ccttgtcacg attcttctcc
     1921 cgctccatat cgataaagtc gacgatgatg atcccaccga tcccccgcag cttgacttga
     1981 taggcaactt ctttcgccgc ctccaggtta ttgcgcaaga ttgtctcttc ttgatcgcgc
     2041 ttgccgacaa accgcccggt gttcacgtcg atgaccgtca tcgcttccgt atgatcaatc
     2101 accaaatagc cgccggattt gagccacacc ttccggctaa gggcccgcgc catttcttgc
     2161 tccactccca aatattcaaa caggctttcg tccttgtcat agaaatgaat ccgcgacgtc
     2221 tgctccggcg agaatcgctg cacgaagtca cgcacggcct catactcttg acgtgaatca
     2281 atccacagcc gttcgacttt tttgccgaac aaatcccgga ccacacgaaa gcttaagctc
     2341 agatcggtgt gcagcaatgc cggcgccgcc tgctgatcgc gtttggtccg gatgtcctgc
     2401 cacagcacgt gcaggaaatc cacgtcggac ctcagctcgt cttctttcac tccttcgctc
     2461 acggtccgga cgatatatcc gcaaccgggg tgccggactc gcttcataat gtccttcagt
     2521 cgcgcgcgtt cctcatctcg tgcaatcctg cgcgatacgc cgatgtgttc gacattgggc
     2581 atgaacacca aatagcgacc aggcaacgac acatacgtcg tcacacgcgg ccccttcgta
     2641 ccgatcgggc ccttcgatat ctggaccatc agctcctgcc cttcgctcag cagctgctcg
     2701 atgggtttgg agctttcccg ccgtggccgc aattcatcgc cgtctttgtc gtcgtcgtca
     2761 gaatccacca gcgtatcgcc gggttcagcg tccatcgaca aatccgacac gtgcatgaat
     2821 gcggccttct caaggccgat gtccacaaag gccgcctgca tgcccggcag caccttcgcc
     2881 acttttccct tataaatatt cccaacgaag tctttgtgct ttgcccggtc tccaaagaga
     2941 tccgtcacca ctccgccgtc caacaccgcc acacgggttt cttcccgtgc gaccgttatc
     3001 gcaatttcaa cacccatact gactccttcc gttcagcggg ccgatcgatt cgacgtgagg
     3061 aaggaacgcg ggaaagccca ggttccgata tcactcaatg tcttgccgaa ccagtcgtcc
     3121 gcacaatgat tagcaggtac ctcgcagcgc cgatccattc gacgccgcac ccacatccgt
     3181 tcaataaaac aaactcaatc gccgccgttt gacgttcagc aacaagccga gcgaggccat
     3241 cgtcatgatg gtcgcgctgc cgccatagct catcagcgga agcggaatgc ctacaatcgg
     3301 aaacatgccg gccgtcatgc caatgttcac caccacgcaa aaacagagca tggagatgac
     3361 cccgaccgcc agaagcgcgc ccaactcgtc tttcgcgcgc gtggcgattt caagcgacaa
     3421 ccatatcagg gccacgaata acagcagcaa gaccagcacg ccgatgaatc cccactcttc
     3481 agcaaacacc gaaaatacaa agtctgtgtg cccctccggc aaaaacttca attggctctg
     3541 ggttcctccg tagagtcctt ttcccgtcaa ttctccagca ccgatggcaa tgcgtgactg
     3601 cagcgcatga taccccttgc ctcccgggtc gtaagtcggg tcgacgaagg ccatgatgcg
     3661 ctggcgctga taatcatgca aggagcccca catgatctcc caggcaaagg gaaagagcat
     3721 cagcgaaaac agcagaatca cgcccagcgc ctgggatcgc atcccgacca tcaacagcat
     3781 cgccgcgtag accgccatga aactcaggcc gcttccgaga tccggctgct tgaggatcaa
     3841 caacagtcct ggcagcatca gagctccagg gatcaccaca cgttgaagcc acccgacccg
     3901 cggggccttt gaatagtagt gggccatgac gagaatcagg acgagtttcg caaactccga
     3961 cggttgaaat gcaaacggcc cgatcggaat ccagcgctgg gctccccgac tcgtcttccc
     4021 ttcaaaaagc acgaccgcca gcaaaatcaa gattatcgcg taggcaggat acgctaatcg
     4081 ggctatgcgg tgatagtccc acaccagcat gatcaagaag gcgacgatcc cgagtagaat
     4141 ccacacaatc tgcttcgcgt agaagggaag gcccggtccg ccctgatcgt gcgtcacact
     4201 gtagatagag agcacgccga tcccgagaat gacaaagatc aaaccgagat aacgcagatc
     4261 gaaactgtcc agcccgcggc gatccatgac acgattgatc ataacgcgcg accctccatg
     4321 gtctgcgttg gagccgatgt tgcggcttct gtgctgcctt tcggagcatt ggacttaagc
     4381 ttcacatagg tttcgatgac ttccttcgcc agaggagcag ccgcagaacc tccgtgtccc
     4441 atgtgttccg ccaacaccgc cacagcgatg gtcgggtttt ccaccggagc aaaggcgaca
     4501 aaccacgcat ggtccctgaa cttcttggga atgtcttttt ctggaccggt gcgaagcgcg
     4561 gcggtctgcg ctgttcctgt tttccccgcg atcgtgacaa tcgacgattt ggctctggtc
     4621 gccgttcctt cagtgaccac gccggctaac gcttcccgaa tcagcttcaa tgtgtccggc
     4681 ttcacatcta atgtagattt cggaacggcg ggtatttcct ggcgttgtcc cgtctcccga
     4741 tccatgatcg cctgcaccaa acgaggacga taggtcaccc cgtcgttcgc aacggtcccg
     4801 atcaggctcg ccatctgcaa cggtgtgaca gtcacatacc cttgcccgat ggcggcggag
     4861 atggtttctc cgggaagcca tgcctcatgt ttgaccttct gcttccaggc cgtcgacgga
     4921 acggttccga tccgttcgga cggaagctca accccggttt cttctcccaa accaaactgg
     4981 tgggcataag atgccatcgt atcaatgccc atgcgctgcc ccacggtata aaaatagacg
     5041 tcgcaggaat ggatgagtgc ttttcggaga tcgacggaac catgcccgct ggccttccag
     5101 tcatgataga cgcgtttacc gaactggtag cctccagtgc agaagaccgt cgagtagggc
     5161 gtcatcgtct tcgattcgag tgccgctgcc gccatcagca ccttaaacgt tgaccccggc
     5221 gggtactgtc cctgggatgc tcggttattc aagggacggc cttcatcctg gacgatctcg
     5281 acccattgtt tggatgtcag ttcgcgcgac agcacattgg gatcaaatgc tggacgactg
     5341 gccatcgcca gaagatcgcc tgtcgtcgga tccagggcaa cgatggcgcc ggattcttcg
     5401 cccaagagat cctccgccac cttctgaagc cgcgcgtcga tcgtcaaata cagatcatcg
     5461 ccggcttgag gctgatcgac gagcaccgta cgtttctcat ggcccagggc gtcaacttca
     5521 atgcttttct gcccggcctg tccccggaca atatgatcga agaatttttc cacgccatat
     5581 tgcccgacga tactgccctg atgaagctcg gcggattccg gcttatccaa ttgttcggcc
     5641 gatatctcgc cgacataccc cagcacgtgc gacgccgtga cgccgctcgg ataattccgc
     5701 tgcgactcga cttgaatcat gacgccgggt agatccaggc gatgcgactc gatcaacgtc
     5761 gcttcgcgaa gagtgagccg gtctttgatt ttccgaggca gttgctttgc ccctcgcaca
     5821 gccaacttct tccggaccaa tgcggggtcc aagccgatca attccgtcag tgtttcaatc
     5881 aacgcctcgc gatctttgac gtcttcaagt gaaacataca gactgaaact gggcacgttg
     5941 ttggcgagca ggactccatt tcggtcgtag atcaaccccc tcgcaggctc caaaataacc
     6001 gagcgcgtgc gattgttctc cgagagatcg cgatagtagg gtccctcgcg gatctgcaga
     6061 tgccacagtc gcaaggccag cagcgcgacg accagcaaca ggcccacacg aagcaccacc
     6121 agtcggcgct ggagttcccc gagttctgaa tcgtgaagac gggctatcgc catgttaatc
     6181 gttgcccttt ctcatcacga gaagcgaccg aatgtcctca aacgttgaac ggcaaatcgc
     6241 tgttccatca gccaaagcac gcctgccccc aacaacgcat cgaagcaggc ttgaggcagc
     6301 acaacagact gtatcatcca ccaggtctcg gacatcgtgg cgttcttgag gctgaacacg
     6361 gcaaccacgc cccctgccag agagagcgtc aacagcccaa tgcacaaaat cgtgggcgtg
     6421 acctgcgcca cctgtcgccc taacagtccg gcgaacaatc cggcgccgcc agtcgtaaca
     6481 atactcaacc acaaatcgcc cgcggaatac agattgagca cccagccgat cgccaagccg
     6541 acgagcagcc cttcgagttc cccggcgata agtccgatca cggcagcaat gacgagaccg
     6601 agatccggct tgattcccca caggctcaca tgggggagca gcgtcgtttg gactgggacg
     6661 agcaagagca cgagtccgag atacaggaga actttcatgg tcgtgacttc gccgcgctgt
     6721 ccgtcttctc ttccaccgct gccgactgtc cctggggcga ttgaatgacc aacacttcct
     6781 ccacacggtt gacatcgact tctggcgtca attcagcggt ttggaagagc gcgccctctt
     6841 ctttggcgat tcccgtgatt gttccgatgg ctagcccgcg gggaaagcct ccgaccagcc
     6901 ccgaggtcac cacccgatcc ccgtcccgca cgcccgataa gagtgggata tacttcagcc
     6961 tgacttttcc ctgcggcgtt ccttccacaa tcccttcgtc cctcgtccgt tgaatgagcc
     7021 cggcgatggc attgttagga tccgtaacga gaaggaccac cgaggttccg cctgtagtct
     7081 tgaccacgcg tcccacgact cccgaggggg taacgacgcc catatccggg tgaatcccgt
     7141 cactctctcc cttattcagg atcaccgaac gataccgatt cgtcgcgtct ttcccgataa
     7201 cctgggccgc caccatcgtc ggcagagcct gctccttaaa ccgcaacaac gtagtgagcc
     7261 gctgggtagc ggatgccgat tcccgcagat gattattctc ccctcgcagc cactcgatat
     7321 cctggcgtaa ccgacgattc tcatcctcga caccttgcag cgtgacatag cgctgccaga
     7381 catcggagat accctgatcg agcgaagcca cggcctcgag cggcagccgg acgagttgac
     7441 cgactggtcc gtccagaaag tccaggaggc cctgactttg gctggggaat aagaagaggg
     7501 cagccaggat gaacgcaaac aatcctagcg ccaggcgcct agctccgtag ggggaacgca
     7561 cattgaccat ccacatgaat cgaggatgga accgttaccg gaaggtgttg gcctgcgaca
     7621 taaccgcgac tttggccaga agatcgagtt catcgagaat tttccccacc cccagcacca
     7681 cagaggtcaa gggatcatcc acggtgatga tcggcagatt cgtctcttcc cggaacctgg
     7741 tgtccatgcc cttgagcaag gagcctccgc cggtcagcac gatgccacga tcaatgatgt
     7801 cgcccgccag ttcaggcggg gtgttctcca aggccacttt gatcgcgttg acgatcgttc
     7861 cgattggttc ctgcaacgcc tccctgatct cggcatcatc caccaccaac gtgcggggga
     7921 tgccggaaat caagtcgcgc cctttaatca tcatggtttt tctttcctca aagggatagg
     7981 cggaaccgat ttcgaacttg atgcgttccg ccatgtgttc tccgatgagg aggttatatt
     8041 tctttttgat gtaattcatg atcgcatcgt ccatgcgatc gcccgctacc ttcacggact
     8101 cgctgtagac gatgccgccg agcgagatca ccgcgatatc tgtcgtacca cccccgacgt
     8161 caaccaccat gttgcccgaa ggttctgtaa tcgggagccc ggccccgatc gccgctgcaa
     8221 ccggctcttc aatgagatac acttcccgcg cgccggccaa ttctgccgaa tcacgcaccg
     8281 cacgttgttc gacttgagta atccgcgagg ggaccccgat gatgatgcgg ggcctgacga
     8341 aggcgcttcg attatgcgct ttttggatga aacgcttgag catctgctcg gccatctcaa
     8401 agtcggcaat cacgccttct ttcatcggcc gcacagccac gatattgccc ggcgtacgcc
     8461 ccagcatttt cttggcttcg gcgcccacgg cgagcacctt ttccgtcttc ttctcgaccg
     8521 ccaccactga tggctcgttc aagacgatgc ccttcccgtg gacatagacg agcgtggtcg
     8581 ccgtgcctag atcgatggca aggtcatttg agaaccaacc aaaaatgtcg ctcgtaaacc
     8641 ccacggtgac tctcccttcg cctcaggctc tacatacaat aacaagttac tagtcaggca
     8701 ttgccagccg accggcatct aagacctggg tgccagccaa ttcatcaccg agtcggcgcc
     8761 cctgctcgtt tccaatgatc aagatcgctt caaacgccac aatcacggca gaaaccagcc
     8821 acccgacata tggccccaca tacggtatgg caaaggccaa ttgggcaacc gcaaacggga
     8881 tattgcggat tatggactcc cgaaatcctg ccgtctcacg cgttcccgga cgaatcgtct
     8941 gcagaccgat cagccgtttc ccaacactcc gaccgccggc aaatccgtcg gccacgagca
     9001 gataggccaa cccggcgaga aacccaacag gagcaatgac ttcagccgca gccgctgcaa
     9061 ggaacaggtc gatcagcttg gcaatgaacc ggttgagaac ttgcgccttg gggtagatcc
     9121 cgatacccaa tggatgtacc ccaacctgct cggccaagtg agctcctcgt aattagtggc
     9181 aaagtataac aaattagatg acctggcaca aacgaatcac cattcttcaa ccggggtcac
     9241 tgtcgcttcg ttaaatgtca ttctgatgca gatcgacgca acttgccttt agccttttct
     9301 ctgggtacaa tccgcgccgt ttcactttgt atcgaggaca tggcaccatg atcaaaattt
     9361 tgcgttccgc gaacgagaag tacccctggt ttcttgccct cacgatgggc ttcatcgcga
     9421 tcagcttcat catcggcatg ggctggtggg gcttcggcga gcatcaaggc acggtcgtgg
     9481 cttccgtcgg agatctcacg atttcacacg atgagtttaa gcgtgcctat gaaaacacct
     9541 accgctttta caaggacaaa gttcctggag aattcaaaga cgagactatc aagcaatatg
     9601 tcgtcgagca gttgatcgac aaccgggttt ggctcatcgc tgccaagaat atggggctca
     9661 cggtcaccga cgaagacttg cgcgacgtca tcatgcagat ccccgaattt cagagaaacg
     9721 gaaccttcga tccggaactg taccaacgtc tcctggcagc caatcacctc acccccgcca
     9781 tctttgaagc catggaggcc aaggaactcc tcagcaaccg cgcacggatg atgatcagtg
     9841 acgccgtcac cctgaccccc agtgaactcg ccgaggccca ggccctcacg ctccggcaaa
     9901 cggagccgga cccagccaag gccgcctccg caaaggaccg tgcgatacag gacgtgctgt
     9961 tccaaaagca acaacgagcc ctgatggcct acacccaatc gctcaaggct acagtcccca
    10021 tcaacatccg ccgtgagatg ttatagccgt ctccgcttgt cgagggcaaa ctgtgatgcg
    10081 ctctcggcta cagaacctgc cttcggcccg catctccttc gtctagaccc aatttatctt
    10141 tcaaggacga cgatgggaaa tcagcatcgc atcgccgtaa ctgtagaacc gataccgccc
    10201 ccgcaccgct tcttcatagg cttttctgag aagatcgacg ccggcaaagg ctgcgaccaa
    10261 catcagcagc gtggtctgcg gtaagtgaaa attcgtcagc agcgcatcca cggcatgaaa
    10321 tgcaaacccc ggcgtgatga acagcctggt ttcgccctga aacgacctca cttccccttg
    10381 cacttgcgcg gcggtttcga gggcgcgcac cacggtcgtc ccgaccgcca cgatcctccc
    10441 gcctgattgt ttcgttcttt ctatcgccct cacggtttcc atcccaacat cgacccattc
    10501 ggcacccatt cgatggtctt cgattcgatc aactgttacc ggcttgaagg tgccgatccc
    10561 gacatgcagc gtgactcttg cgaggccgat cttccgatcc gccaatcgag ccagcagcgc
    10621 ccgggtgaag tggaggccgg ccgtcggtgc ggcgatcgca ccttcatcat gggcaaaaat
    10681 tgtttgatac caatccctat ctcggtcatt cggcgtccgc ttgatataag gaggcaacgg
    10741 cattcggccg tggatccgga aaaattcatg cgtcgacagc ggcccttcaa tccgaacgac
    10801 cgtccgcccg gcatctcggc tcaacaccgt cagacacatc ccgccgtccg catcgatttg
    10861 ttggcctgca cgccatttcc ccttcatcaa aacttcccaa gaccgctcgg ctacttcttt
    10921 cacaaagagg atctcgaccg gccgtcctga aatccgtttc cgtcccatga cgcgagcggc
    10981 tcgaaccttc gtatcgttca ccacaagcag atcgccgggc gtcagcaaat cgggaagatc
    11041 ggccaccttc cggtggctta atgattggct ggtcgggttc agtacgagga gtcgcgcttg
    11101 gtctcgcggg agcacaggct cggtcgcaat gagcgccgga tcgaaaggaa agttaaactc
    11161 ggaaagctgc atcagaaaga aggcgggacc ggctgcttcg tcagtgccat gctcttcaaa
    11221 tcggtaccgg gatagtagta ttccaggatc gtcgaaaagg gataccctaa ctctgccagt
    11281 tctttcgccc cccattggca catccctacg gcatggccgg ctccgtaccc ggaaagcagg
    11341 atctcctggc cgacggaatc gatcgtaaac tgtgtgctcg gcacaatcga atacccgacc
    11401 gctttgcgaa agtcctcgcc gcgcaacacc aattcccctc ctgaatgcac caccagcagc
    11461 ttggctactc gtcccccgcg gctaaacgcg agcggcgtta tcgaggcaat cgttccgaca
    11521 gcaaatccct gccggcgaag attttgttct agcgcatcca gtttgaacga aaccttccac
    11581 tgatagtagg gggactccag atcaaaggga cattccacgc ctttgaggta aggatactct
    11641 ttggaccaaa cgttgacggc atcttctgtc aggccagccg ctgttgaaga gaacgccgca
    11701 taaatcggtg cattctgata ggtcaggacg agccctcgag tctcatccac cgcacgcagc
    11761 accgctgcat caattccatt cttgcctcga tacacttgat cctgcaccgt cgccgcgaca
    11821 tcgtagtcgc gtgttgcgct cagcatctgc tggtacaagg cataggttcg cgccgccacc
    11881 gcttgggcct tgagcatctc cggatgccag gtcgaattga cttccgccgg caccacaccc
    11941 ttgacgtatt cttccaggtc gacttgattg atgaccagca ttccatcacc ccgccggacg
    12001 acatgtacca ggccactcac cgggagggcc aggcgggact cgttcgccgg tttcgatgtt
    12061 ccatgagctt ttccgtttgg cttggccagc aacaatgtta ggccttgttc acctccgctc
    12121 aatgtcagtc gctcgtgggc catgcgaacg ccgttgacca gaaacccctt tccgtacggg
    12181 atcacacgaa ccgtcgaatg gatggctcgg ccctgacgac ttgaatcagc cacccatacg
    12241 gcaccctccg cctggatatc catcttgtga acgtctgcgg caagcagcac acgaatcgat
    12301 tgtgccccct cagcccagga tgctgcgaga gtgaggagga aacagacaat caatccggcc
    12361 cttaccctgt agaatcgtga gttcatcgac ggaatatcca tgacactaga tagaatagca
    12421 gactgaggac cacactgagg agcaggctcg tcccgagagg gaaaaacagg ctgaagttgt
    12481 ctcgtttgat ggaaatatca cctggcagct taccgaacca gccgaacagg ctcccgattc
    12541 caggcacgcg atcggcaagc aggaacagca cgcccagcac ggccatgacc gctccgattc
    12601 cgatgaacag cttcccaata cttgcccact cgaccatcag cctttcacca gacactcggc
    12661 aatctgaacc gcgttgagcg cggccccttt gcggagattg tcggatacaa cccagaggtt
    12721 cagaccgttc gctattgatt catcttccct gatacggccg acgtacacct cgtccttgcc
    12781 ggtcgcatcc aaaggcatcg gatagagctt cttactggga tcgtcgtaca caattacgcc
    12841 cggcatggct gccagagcgg cacgggcttc attggacttg agcggacgtt ccaattccac
    12901 gttgatggct tccgagtggc aacgcaggac cggaacccgg acggtcgtcg ccgtaacgcg
    12961 aagcgccggg gcctcgagaa tcttcctggt ctcacgggca attttcactt cttcggagca
    13021 atctccgcct tcagtgaagg atccgatatg cggcagcagg ttgaacgcga tctgataggg
    13081 atagacctcg gccttcgcgt cgcgaaatgc catcagatct ttcgtctggg tcatcagttc
    13141 atccatggcc gccgcgccgg tccccgagac cgattggaac gtcgtgacga cgacacgctt
    13201 cactcccacg gcatcctgta acggcttgag cgccatgacc agcggagtgg tggtgcagtt
    13261 ggggatcgac acgatgccgc gaggcatcct cgtcagggca tgggcgttga cttccggcac
    13321 gaccaacgga acgtcatcct ccatccgaaa gaccccgctg tcgtcgatga ccacgactcc
    13381 ggcagtcccg agccgttgac cataatcgcg actgatcgca tccgtggctg aaatgagcgc
    13441 gaagtcgacc ccgacaaacg acgactccgg tgtcagttcc tccaccttcc attctttccc
    13501 ctggcaggac atcacttcgc cggccgaacg cttcgacgca aagagacgca gcgaggccag
    13561 cggaaactct ctttcttcca aaatctcgag cgtttctttc ccgaccgccc ctgttgcgcc
    13621 gagaatggca accacgtatg cggatttctt tttcagcatc aatgccatcc tacctataag
    13681 acgatctcac gaatccgatg attaaacgtg tcggcgacga agagatgtcc gtcacaatct
    13741 acggcgaccc cgaacggata gttcacactg gcttccagag ccgttccccc gtctccgcca
    13801 taattcgcta cccccacacc agccaaccga tccaaccgac cggttgagcg gttccaccgc
    13861 ctgatcaaat gattgtccga atccgtcagt aagatatttc cctctgtatc gacggcgatc
    13921 cccgatggtc tggacagact gctcgaaggc ggctcaacag ctccttgata gcgatagtcc
    13981 cctccgtctc ccgcaaccgt gttgatcatg cccgtggcag catcaacggc acggatccgc
    14041 ccgttaaacg tgtctgcgat aaagagggtg ccgtctttcc caagcgccag gccgctggga
    14101 ccagccagcg cagcctccgt cgcgggaatg ccgtctccat tgtaactggc caatcctata
    14161 ccggcgaccg tgcgtatgag ccctgtggaa agatcaattg ctcgcactcg attgttgctc
    14221 tggtcggcaa tatacagcgt tcctgactca ctcaccgcaa gcgcagcggg ctcattcaaa
    14281 cctgcagcga cagccggtcc gccatctcca ccgtaccgcg gctggccgac gccggcgatg
    14341 ctcgtaatca agccggtacc ggcctccaca cggcgaatcc gatgattcat cgtgtctgca
    14401 atgtagagat tccccgcctt atccacagcg acggcagtcg ggaaattcaa cagcgcgcgg
    14461 tccgctcgcc ccccgtcccc gccgaagcgt ctggcagttc cagctccatt gacgacatag
    14521 cgtactgttc cgctcaaatc tgtctgctgg gcgaaggcac gctcgcccgt cgtaccggca
    14581 tccgagaaag ggtcgtcacc tgccggttcg ggaactgagt gggcttttac cacttcaagt
    14641 cgaggttcag ggactccaac atagccggcg atcgttctga tctgcccggt ttcccgattt
    14701 acggcccgaa ccagatggtt ctccgaatca gcaataatga gattcccacg cccatccacc
    14761 gtgaccgcct tgggctcgtt gagacaggct gccgtcgccg gtcctccgtc tcccagaaaa
    14821 ccgctctcac cggttccggc cacagtgcgg atcgtcccca ttttgacacc ggtcgacatc
    14881 actcaatcct caccggtcaa ttcaagttcc gaatgattgc gtcgcccatt tccgtcgtcc
    14941 cgacgagacg ggtgctagga ccctggatat ccttggtgcg gtagccgagg tccagcgttt
    15001 tcacaatggc ttgctcgatc acatccgcct ctttatccag cttaaaggcg tacgacaaca
    15061 tcatcgctgc cgatgcgatc gtggcgatgg gattcgcgat attcttcccc gcaatatcag
    15121 gggcacttcc gtgaattggc tcatacagcc ccactgccgc accgacgctg gcggaaggca
    15181 acataccgat tgacccggta agcatcgcgg cttcgtcact gagaatgtcc ccgaacatgt
    15241 tgttacagag catgacgtcg aactgacggg ggttgcggac cagctgcatg gccgcattat
    15301 cgacatagat gtgattgagc tcgacgtccg ggtagtcctt ttgcacctca atgacgacgc
    15361 gacgccatag ctcggatgat tccagcacat tggccttatc gactgatgtc accttcttcc
    15421 ggcgtttgcg cgccgcctcg aacgcgacct tggcaatacg ccggatttct tccgtggtat
    15481 agacctcggt attgatgccg cgctcgctgc cgttcggcat tttctcgatc cccttcggct
    15541 ttccaaaata gatcccgccg gtcaactcgc ggacgacgag gatgtcgatc cctgcaatga
    15601 cctccttctt gagcgttgac gcatcggcaa ggacgtcata caacttggca ggacgcaaat
    15661 tggcatagag ccctaactgt tcgcgcaatc ccaataaggc ccgctccgga cgcaggctat
    15721 actcaaggcc ttcccacttg ggtcctccta cagcacccaa aagcaccgcg tcgctttgct
    15781 tcgccaatgt gagcgtatcc tgcgggagcg gcaccccgac tttatcgatg gcttggcccc
    15841 caacatcacc ggccgcaaat tcgaactgat gcccgaattt ttcggccaca accttcaaaa
    15901 ccttgacggc ctcggggaca atctcgcgcc caactccatc accagctaag actgcaatct
    15961 tcgctttcac tgagtgctcc ttttttctat tctaatacct gctcgtcttc cactttccac
    16021 gccggttcga caacacagag aaactcgatc tcggagtttc cggtattttc cagtgactgt
    16081 ttcccccctg gcggcacata gagcgtggtt cccggctcca ccgacagcac ctggtcatcg
    16141 atcatgaatc gtccctggcc cgcgatgaaa tagtagacct ccgatgaggc caagacatgc
    16201 cacttggatc gccggccagg ccccaacgtg ccgtgcgcaa gactgtagcc caaattcagc
    16261 tgatgcttgg ctgggtggag aatttcccgc agacgtgtat ggtcgcccgc cagaaattcc
    16321 gggcgatccg atggtgtgac cttgaacacg ggtgcccccg ccatgcaaca gcaccggcca
    16381 aataagccga tcactgtacc gtgaggggtt tactcaaatc aagttgaccc tctgctcgcc
    16441 ctgcgcctgc ttcgcggcca aataggccag cttattcaac gcgctgaggt acgcccgggc
    16501 cgaagccgta atgatgtcag tgtcggcccc atggcctgaa acggtccggc catcctcctg
    16561 aacccgaacg gaaacctctc cctgggcatc ggtccctccg gtaatcgcct tcacgacata
    16621 catgagcagc ttgctcttgg tctgggtgat cgccgcaatg gtccgataca cggcgtccac
    16681 cggtccatca cccgtcccgg tctgggtgat ggacttgcca tcgatttcaa gttcgaccgt
    16741 ggcggtcggc acctgatctg ttccactcgc gacatgaaac gatttcaagg tgatccgatc
    16801 agccatcttc gccaattctt ccgatacaat cacctcgagg tcttcctcgt aaatttcctt
    16861 cttatgatcg gccaacttct tgaagcgctc gaacgcatgg ttcacttctt cgtccgtgag
    16921 cttgtacccc aattcctcaa gccgctgacg gaacgcatga cgccccgaga gcttgcccat
    16981 taccatcttg ctctcgacca gtccaatcga ttccggccgc ataatttcgt aggtcgtctt
    17041 gtccttcagc agaccgtcct gatggatgcc tgaggtatgg gcaaaggcat tcgcgccgac
    17101 gatcgccttg ttgggctgca cgaccatccc ggtgatcttg ctgaccaacc gactggtctt
    17161 ggaaatttct tccgtgtgga tctttgtgtc cgcttgataa agatccctcc tcgttcgcaa
    17221 ccccatgacg atttcttcca acgaagtatt gccggcccgt tcaccgatgc cgttgatcgt
    17281 gcactcgacc tgaccggctc cttccattac ggcggctaag ctgttcgcca cagcaagacc
    17341 caaatcgtta tggcaatgca cagagattac ggctttggag ctattcggca catgctcacg
    17401 aataccccga atcaacttgc cgaattcctg cgggatggca tagcccacag tatccggaat
    17461 attgaccgtc cctgctccgg ctgcaatcac ggcctcgatg acttcgtaga gatacgccgg
    17521 gtcggaacgg ctcgcatcca tcggtgaaaa ttcgacgtcc tccacatagc ctctcgcccg
    17581 ctggaccatt tcgactgcgc gcttcttcgc ctcatcacgg gtcatcctga actgatgctt
    17641 caaatggata tcggacgtcg agagaaaggt gtgaatccgc accttcggcg cccctttcaa
    17701 cgcctcccag gcacggtcga tgtcctccgg acgagcgcgg gcaaggctgc agatcgtcgg
    17761 accttcgacc tcctgcgcaa tccggcgaac cgcctcaaaa tcgccgggag aactgtacgc
    17821 aaatcccgct tcgatgatgt ccacgccaag cctggccagc tgcttcgcca ccatgacctt
    17881 ctcttccacg ttcatgctcg cgccgggcga ttgctccccg tcccgtaagg tggtgtcaaa
    17941 aattcttatc atgcgtgtca tggcgtcacc tttctccctt caacccctaa aaaacacaaa
    18001 agcctccatc cctgcaggga cgaaggcttg acgcgctccg tggtaccacc ctggtttggt
    18061 gtggaacgac cggccggtca tccacaccct tcgttcggcc cgtcacgggg gcaaagcgga
    18121 atccattact ctcggttcac ggattctggc tcggaggcga gttcggtgac gcacgggctg
    18181 gtttgcacca tccaccagct ctctgaaaac cgtgcgagtc ccctactact cctcgtcgct
    18241 gccgctacga tttctggtct actgccggct gctccctctt taactgaggc tgcttccctg
    18301 caagttttgc aaacattccg agcattcgtt cgaacggacc gagcagggca taccctgcaa
    18361 agataacaaa aagcataact tgaggccagg ccgccaccag taccaagccc aaaatgcccc
    18421 agaccaaata ggtaatgtgc tggccgcccc ggaacttcat atccttgaag ctccgatact
    18481 tgatcgtgct caccatcagg aacgaaagca ccagcgtaac gattagcacc acgatcggtt
    18541 tgacctccgc accggtccga acgatgtggt gatcgaaaat caccatcgac gcgaccacgc
    18601 cggctgcggc agggattgcc agccccgtaa agtatttccc gtcggatacc gcgaccgtgg
    18661 aattgaaccg agcgagccga acggccccca tggccacata ggcaaacatc acggcgatcc
    18721 cgaacgtccc ctgtccgctg agcgcccaag aatagatcaa cagaccaggg gctacgccga
    18781 acgagacgac gtcggatagc gaatcgtatt ccagaccgaa atggctcgtg ctgttcgtca
    18841 gccgcgcgga cttgccgtcc aacacatcga ataccatggc caccagaatc gcgacggcgg
    18901 ctgccaggta attcgcattg aacacggaca ggatggcaaa cactccgcaa aacagattac
    18961 cggtcgtgaa caggttaggg atcaagtgca tcgcggcctt atgccgcttc ccgcctttgg
    19021 cgaaagattg tcgaagtcca tttgctttca tcgtagttct cccaaaatgg tttccccccc
    19081 ctttacatga tccccgaccg ccaccttgat caccgtcccg atgggaagaa acgtgtccat
    19141 ccgcgaccca aatcgaatca acccgaaccg ctcacccctc acggcctttt ctttcggcga
    19201 gacccaacag acaatccgtc tagcaatcaa cccagcaacc tgaacacaga gcactttggc
    19261 accctgtacc gtcttgatca tcaccgcatt ctgctcgttc tgtatggtcg cttccggttt
    19321 gctggcaacc atgaacaaac ccggctgata ctggacatcc tccacgagtc cttcgcaggg
    19381 gatccggttg atgtgcacat cgaagacatt gaggaatatg ctgagcttga tgctgcgatc
    19441 tttcaggtat cgcggttcaa actcctcttc gatggcaatc actttcccat caccgggtgc
    19501 cacgaccagc gcagccccct gagggatcac gcgggccggg ttacggaaga accaggagac
    19561 gaagagggtc agaagagccg ccccgaccgc tgcaaccgtc catcccagcc agcctacgag
    19621 cagtgtaacg cccgccgctg ctccaatgaa gggaaatcct tctttggcga acgggacgcc
    19681 gacggcacgg tctgccacag cttcctcaat ccttagttct tggtcttatc gacaagctgg
    19741 tccttcttga gccacggcat catcgcccgc agccgggcgc cgacttcttc aatcggatgg
    19801 gcttcccctt tggccagcaa tgcattgtag acagggcggt tcgcctggtt ctccaggacc
    19861 cattccttgg caaactggcc ctgttgaatc tcggcgagaa tcttcttcat ttcctgtttg
    19921 gtctgctccg tcacgacacg tggacctcgg gtgacgtccc cgtattttgc cgtcgtgctg
    19981 atggaatagc gcatgttggc gataccgccc tggtaaatca gatcgacgat caatttcact
    20041 tcgtgtaagc actcgaaata ggccatctcc ggtgaatagc cggcttccac aagcgtctcg
    20101 tacccggcct ggatgagcga ggtgagcccg ccgcacaaca cggcctgttc gccgaagagg
    20161 tccgtttcgg tttcttctct gaaattggtt tcaatcacgc cggcgcgtcc cccgccgatc
    20221 gcgctggcat aggccaaccc gacttgcttg gtcgtaccgc tgggatcctg atggatcgcc
    20281 aacaaacagg gaaccccgct ccccttcgta tactccgatc gaaccaagtg accgggcccc
    20341 ttgggcgcca ccatgaatac attgatggtg gacggcggca caatctgtcc gaaatgaata
    20401 ttgaagccgt gaccgaaggc cagataggag cctggcttca gattcggcgc gatctcctga
    20461 cgatagatcg aggcctgcgc ctcgtcgggc gcgagaatca tgatgacatc cgacgccttc
    20521 acggcatcgg cgaccggcat gactttcagc ccgctctgtt ccgccttgcg ccaggaactc
    20581 ccttcgcgca atccaatcac gaccgaaacg ccgctctctt tcatgttcag cgcatgggca
    20641 tgaccctggc tgccgtagcc gatcaccgcc acttttttat tcttgatctg ctgaatatct
    20701 gcatccttgt cgtagtaaat cttcatcgat gactcctcgg tcacgctcgg ttgatacgat
    20761 agatggatcg cggaacggct cggggcctta ttcgcgagca actttcttgg gttgtgccga
    20821 cgacgagcgc agcggctcgc gcgccacggc gacccggccc gttctggtca gctccttgat
    20881 cccaagcggt tgtagcagat tgatgatggc ttcaatcttc ttgggatcgc ccgtgacttc
    20941 gattgtatag gtagccggcg tcgaatcgat cacgttggcg cggaagatgt ccgcaatcct
    21001 caaggcttcc gcacgatctt cctgtctggt gtgcaccttg atgagtgccg tttcacgcga
    21061 cacgaaatcg ctttcgttca aatcgacgac cttgatgacg tcgatcagtt tattgagctg
    21121 cttcacgatc tgctcgacaa tccgttcgtc cccggaagtc acgataatca tttgcgacat
    21181 tgacggatcg agcgtgggtg cgacggacag actctcgata ttgaatcccc gcccgctgaa
    21241 caatccggcg atccgggaca gcacgccaaa cttattttcc actgtaacag agataatgtg
    21301 ttccatatgc ttcgcgactc ccgacattct taggccgtca gaatcgtgtc agaatccttc
    21361 ggagcggtct tgccggcccc tgcttgtttg ttcttcaact ccggcggatc ctccagaatc
    21421 atctcgtgat tgcacccgcc ggccggaatc atcggataca cattctcgta gggataggtc
    21481 ggcacatcca cgatgactgg tccaccgatc gcgatcgctt ctttcaggat gccatcaaga
    21541 tcgcccacct tccccgcgcg caagcccact gcaccatagg cttccgccaa tttaacaaag
    21601 tccggcgtcg tgtccaggta gctcgacgcg tagcgtcctt catagaagag atcctgccac
    21661 tggcgcacca tgccgtggaa ccgattattc aggatgatga tcttcaccgg cagcttgtgc
    21721 accacggctg ttgccatctc ctgcatgttc atttgaacgc ttccgtctcc ggcgatgcac
    21781 agcaccagcc gattccggaa cgccgcctgc gcgcccatgg ccgcaggaaa tccaaacccc
    21841 atcgtcccca acccgccgga ggtcagccac cgattgggct tcgccagctt aaagtactgg
    21901 gccgcccaca tctggtgctg cccaacgtcg gtggacacaa tgggatcccg atcgttcgtc
    21961 agctcataca gccgcttgat tacgtgctgc ggcttgattg gcccgtcctt ttcctgctgg
    22021 taggtcaaag gatgtgttcg ctcccattcg cgaatctgat cccaccaggc ttttcggaga
    22081 tccttctgct cgccgttgac ggtcgccttg agaatttgat tcaactcgcg caacaccgcc
    22141 ttgcaatcgc cgacgatggg aatatcgacg tggatatttt ttctgatcga tgtcggatcg
    22201 atatcgatat ggacgatttt ggcatagggg caaaactccg agactttccc cgtgacgcgg
    22261 tcgtcaaagc gcgcgccgat ggcaacgaca aggtcggcat agtgcacggc catattcgct
    22321 tgataggttc catgcatgcc catcatcccc atcgacaacg gatgttcgcc gggaaaggcc
    22381 ccgagcccca tcagcgtcat atccacggga atctgagtta attcggccaa ctcgatgagt
    22441 tcctgcgacg cacccgaaaa gacgaccccg ccgcccacat agagaatcgg cttcttggcc
    22501 ttcataatgg cttcggccgc ctgcttgatc tgccacttgt tcccgtcgta ggtcgggttg
    22561 tagcccctga tagaaacgga gttgggataa ttaaattccg ccttctccat cgacacgtcc
    22621 ttaggaatat ctaccagcac agggcccggc cgtccggtcg tcgcgatata gaacgcctct
    22681 ttgatcgtgg acgcgaggtc gttgacgtct ttgacgagaa agttgtactt cgtacaggga
    22741 cggctgagcc cgacgttgtc cgcttcctgg aaggcgtcgt taccgatcaa actggtcggc
    22801 acctgtccgc tgaaacagac caacggaacc gaatccatat aggcatccgc caatgctgta
    22861 atgacattgg tcataccggg tccggacgtc acaaggcaca cacccgcttt accagtcgct
    22921 ttcgcatacc cttccgccat atggccggca ccctgctcat ggcgagtcag gaccacttcg
    22981 atgtcctttt gctgatgcaa cgtgtcgaaa atcttcaaga cgacaccgcc cgggagcgca
    23041 aaaatcgttt tcacaccctc tcgtttgagg cattcgatga atatttcagc gcctgtgagt
    23101 ttcatccaaa cctcctctcc tcgttcgctg gcgtcagcga gtggagacac cgaacacatt
    23161 cgggggattc aattaaagac accttccccc cgatcattca gaattttctg aaaaatcaag
    23221 gggaaagatc cgagatccta ccatcccgcc ctgtggaggt caagggcgcg agagaagacg
    23281 aaggcctttg gcaaggccgg acgccgcgtg ctacctttcc ttttattatc ctctatgagc
    23341 ccgctcaaat tgacgataat ctcgcggatt gactgccacc tctgcgacgt cgtgtatcgt
    23401 atggccctgc atcttcaagc agattttccc atgcagatca cgaaagtctc cgctgagggc
    23461 aatccaccgc tcctgactcg ctacggtgca cgcgtaccag ttgtgctgat tgacggcatt
    23521 gaacgatgcg ccgggaaatt cacggaaggg gacctgcggc gggcaattaa aagagcgcgg
    23581 tggagaagac ctataagccg gattctgtcc cttctgggat atgctcccag aagggggtga
    23641 tcatttctct gggattcgag ttacctcgaa cctcaagcga cctacccgaa gacctcagcc
    23701 gggccagccg gtcgccgctc ccgaaaccgg aaacggcagc tgtcttccta tttggccttg
    23761 catcgagcga cgcttaccct gccggtgatg tcaccatccc cgcggtgggc tcttaccccg
    23821 ccgtttcacc cttacctgat ccgcagcgac gagattgggg tcctatcgca ttggccatcg
    23881 gcggtctgtt ctctgtggtg ccggtgtcgg atcgctccgc ctgggcgtta cccagcgctc
    23941 tgcccacgaa gtccggactt tcctctcgtg acgttaccgc cacgaacggt cacccggtct
    24001 tctccctcgc gcagatccag tatagaaaac cctcgggc
//
LOCUS       DATIFR010000018         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_9692, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000018 DATIFR010000000
VERSION     DATIFR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9692"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VLD60_00745"
     CDS             <1..651
                     /locus_tag="VLD60_00745"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HSB43503.1"
                     /translation="GLVGVQLSGPLTVRTVISQGCRPIGERFMVTRSEHNLIYELSGK
                     PALERLQDAFESLDSPQRRNAHRALHVGLVIDEHRSRFERGDFLVRNLVGADQQAGAI
                     AIGDIVQEGQTLQFHLRDAKSASEDLQVLLAADRAKHQNQPLGALLFSCCGRGEGLFG
                     QANHDSRVIQERMGRIPVAGFFAQGEIGPVGGRNFLHGYTASVAIFAQPDLREEPQ"
     gene            751..1119
                     /locus_tag="VLD60_00750"
     CDS             751..1119
                     /locus_tag="VLD60_00750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSB43504.1"
                     /translation="MPFEEAGPGGKEVTTSSGLQYIDLIVGTGATAQAGQTVTVHYTG
                     WLENGKKFDSSLDRGQPFSFPLGAGRVIKGWDEGVQGMKVGGKRKLTIPSNLGYGARG
                     AGGVIPPNATLIFEVQLLGV"
     gene            1156..2256
                     /gene="gcvT"
                     /locus_tag="VLD60_00755"
     CDS             1156..2256
                     /gene="gcvT"
                     /locus_tag="VLD60_00755"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HSB43505.1"
                     /translation="MKHTPLIEQHRAHRAKLVDFAGWEMPIQYASVVDEYHTVRTKAG
                     LFDVSHMGRITVSGSGSCAVLQRVTTNDVAKLGVDQAHYSMVCNEHGGIKDDIFVYRI
                     ADSEYLLCVNASNREKILSWIKEKRQTGNQCQIEDRTMSLAQLAIQGPASRDILLAVG
                     AEQAAGLRLHHACESRLAGITCFVARTGYSGELGYELNVPAEKASELWRQLLLTGEPK
                     GLKPAGLGARDLLRLEMGYLLYGNDIDEDTTPIEAGADWTVNFQKGDFVGREALKAQA
                     QTGPTRRFAAFELLEKAVPRHGFKILDPSSSQPIGEVTSGNLSPLLQKGIGLGYVPSS
                     FATVGTAVAIEIRGKVLPAAIVKPPFYKKPKS"
     gene            2256..2771
                     /locus_tag="VLD60_00760"
     CDS             2256..2771
                     /locus_tag="VLD60_00760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSB43506.1"
                     /translation="MTKNIYHGKVITLNVDTVTLPNGVTVDLEMVRHPGAAAIIPLKE
                     DGTVILIRQFRHAAGGFIYEIPAGKLHPGEDPKACAARELEEEIGYQAGSLELLSSIF
                     TAPGFTDEVIHIYKATAMTKGRQRLDRDEVLEVVEMPLQEAMEMIRVGTIRDAKTMVG
                     LQSVFIGNGNR"
BASE COUNT          649 a          823 c          827 g          538 t
ORIGIN      
        1 ggactcgtcg gtgtccagct ctccgggccc ctcaccgttc ggacggtgat ttcgcagggc
       61 tgccgtccca tcggcgagcg attcatggtg accaggtccg agcacaatct catctatgaa
      121 ctgagcggaa aaccggcgct cgaacgcctc caggacgcat tcgagtcttt ggacagccct
      181 caacggcgaa atgcccatcg ggcgttgcat gtcggccttg tcatcgatga acatcgaagc
      241 cgcttcgagc ggggcgattt ccttgtacgc aatctcgtcg gagcggacca gcaggccggc
      301 gccatcgcca tcggtgacat cgttcaagag ggtcagaccc tccagtttca tctgcgcgac
      361 gcgaagtctg ccagcgaaga cttgcaggta ctcctcgctg ccgaccgtgc caagcaccag
      421 aaccagccgc tgggagccct cctgtttagc tgctgcggaa ggggagaagg actcttcggc
      481 caagccaacc atgatagccg cgtgatacag gaacggatgg ggcggattcc cgtggcaggt
      541 ttcttcgccc agggcgaaat cggcccagtc ggaggacgga attttctcca cgggtatacc
      601 gcgagcgtag cgattttcgc gcaaccggac cttcgcgagg agccgcaatg attcgtctcg
      661 cgtgctaatt ggatccgcct actactcaga ctcacgcgta agccttgtga gatctcgtcg
      721 ctcgacacaa caatcagaaa aggagtcgac atgccgttcg aagaagcggg tcctggaggg
      781 aaagaagtca cgacctcatc cggattacag tacatcgatc tgatcgtcgg cacaggagca
      841 accgcccagg ccgggcagac cgtcacggtg cactataccg gctggcttga aaatgggaag
      901 aaattcgaca gttcgctgga ccggggccaa cctttctcct ttccgctggg agcagggcgc
      961 gtcatcaagg gatgggacga aggcgtccag ggcatgaagg tcgggggcaa acggaagttg
     1021 accattccat cgaacctcgg ttacggcgcc cgtggtgcag gcggcgtcat tcccccaaat
     1081 gccacgctta tctttgaagt ccaactgttg ggagtataag cacccaactc attgtctcat
     1141 tcagccgaac aactcatgaa acacacaccg cttatcgaac agcatcgcgc ccaccgcgcg
     1201 aaactcgtcg atttcgccgg ctgggaaatg cccatccagt acgcgagcgt cgtcgatgaa
     1261 taccacactg tccgaaccaa ggccggactc ttcgacgtca gccacatggg acggatcacg
     1321 gtatccggct ctggcagctg cgccgtcctc cagcgggtca cgaccaacga cgtggcaaag
     1381 ctgggtgtcg accaggccca ctattcgatg gtctgcaatg agcacggcgg aatcaaagac
     1441 gacattttcg tttatcgtat tgcagattcc gagtatctcc tctgcgtgaa tgcgtccaat
     1501 cgcgaaaaga ttctcagctg gatcaaggag aagcgccaga ccgggaacca gtgccagatc
     1561 gaagatcgca cgatgtcgct cgcacagctt gccatccaag gtcccgcgtc acgagatatc
     1621 ctcctggcag tcggcgccga acaggcagcc gggctcagat tgcatcatgc ttgtgaaagc
     1681 cggctagccg gtatcacgtg cttcgtcgcc cgcaccggct acagcggtga attgggctat
     1741 gaactcaacg tcccggcgga aaaggcaagc gagctttgga ggcaactcct gctgaccgga
     1801 gagccgaaag ggctcaagcc agctggcctc ggagcacgag atctcttgcg cctggagatg
     1861 ggctatctgc tgtacggcaa cgatatcgat gaggacacga cgccgatcga agccggagcc
     1921 gattggacgg tcaattttca gaaaggcgac tttgtcggca gagaagcgtt gaaggcgcaa
     1981 gcacagacgg gacccactcg ccggttcgcg gcttttgaac tgttggagaa agccgtaccg
     2041 cgccatggat tcaagatcct tgacccctca tcctcacaac cgatcggcga agtgaccagc
     2101 ggaaatctct cacccttgct gcagaagggg atcgggttgg gctatgtccc ttcgtccttt
     2161 gcaacggtag gaaccgccgt tgccattgag attcgaggga aggtgctgcc ggccgctatc
     2221 gttaagccgc cgttctataa gaagcccaag agttgatgac caagaacatc tatcacggca
     2281 aagtcatcac gctgaatgtc gacaccgtga cgctgccgaa cggcgtcaca gtcgatctgg
     2341 agatggttcg ccatcccggt gccgcggcga tcatccccct gaaagaggac gggaccgtca
     2401 tcttgatcag gcagtttcgc catgcagccg gaggattcat ctatgaaatc ccggctggga
     2461 aacttcaccc cggcgaagac cccaaggcct gtgcagcacg cgagctggag gaagaaatcg
     2521 gttaccaagc aggcagcctc gagcttcttt cgagcatctt caccgcacca gggttcaccg
     2581 atgaagtcat ccatatctat aaggcaaccg cgatgacaaa aggacggcag cgtttggatc
     2641 gcgacgaagt gctggaggtc gtcgagatgc cgcttcagga ggccatggag atgattcgag
     2701 ttggaacgat tcgcgatgcg aaaacgatgg ttgggttaca gtcggtcttt atcgggaacg
     2761 ggaaccggta ggagaaccgc ttaaggagcc ctccgctttt ggcggaaggc ccctcaagcc
     2821 agatagcctc gccaaaa
//
LOCUS       DATIFR010000019         1745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2500155, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000019 DATIFR010000000
VERSION     DATIFR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500155"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /gene="rplE"
                     /locus_tag="VLD60_00765"
     CDS             complement(<1..289)
                     /gene="rplE"
                     /locus_tag="VLD60_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSB43507.1"
                     /translation="MAKVEKGRSGKPSERKTAKKDAAPQQLDQGSEESKHKPRLRDMY
                     QQQVVPALMKEFGYKNVMQVPKLERVVLNVGMGEAIQNVKLLESAVIELGTI"
     gene            complement(304..633)
                     /gene="rplX"
                     /locus_tag="VLD60_00770"
     CDS             complement(304..633)
                     /gene="rplX"
                     /locus_tag="VLD60_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSB43508.1"
                     /translation="MQALRKSRIRKGDTVVVISGRERGKTGKVLSVDLGAGKVIVEKL
                     NIIKRHTKPNQKAKQGGILEREAPLQISNVMFFCPVTQRPTRVGVRTLEDGRRVRFSK
                     KSNETVE"
     gene            complement(656..1024)
                     /gene="rplN"
                     /locus_tag="VLD60_00775"
     CDS             complement(656..1024)
                     /gene="rplN"
                     /locus_tag="VLD60_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSB43509.1"
                     /translation="MIQNYTYMDVADNSGAKQAMCFHVFGGTRRRYASLGDIVVVAVK
                     EAIPQASVKKGDVSRAVIVRTTKEVRREDGSYIKFDRNACVLINKDGEPIGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            complement(1062..1328)
                     /gene="rpsQ"
                     /locus_tag="VLD60_00780"
     CDS             complement(1062..1328)
                     /gene="rpsQ"
                     /locus_tag="VLD60_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSB43510.1"
                     /translation="MTDGTVKKREWVGRVLSNKMNKTVVVAVERSVIHPVYRKVLRRV
                     TKMKAHDEQNVCRIGDQVRMVETRPISKEKHWRVVEVIQKGRAD"
     gene            complement(1331..1543)
                     /gene="rpmC"
                     /locus_tag="VLD60_00785"
     CDS             complement(1331..1543)
                     /gene="rpmC"
                     /locus_tag="VLD60_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSB43511.1"
                     /translation="MDLKELRQLTGPELADKEKQLVQELFNLRFQFGTGRLENPMQIR
                     KTKRDIARLKTVMTEVSKKEAKAKGL"
     gene            complement(1580..>1745)
                     /locus_tag="VLD60_00790"
     CDS             complement(1580..>1745)
                     /locus_tag="VLD60_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HSB43512.1"
                     /translation="KGKGNPEYWVAVVKPGRILYEMDGVTPEIAKEAFRLASHKLPVA
                     TRLVVRGEFS"
BASE COUNT          334 a          584 c          381 g          446 t
ORIGIN      
        1 tgatcgtccc caattcgatg accgcgctct ccagcagctt tacgttctgg atcgcctcgc
       61 ccatgccgac attcaggacg acacgctcaa gcttgggcac ctgcatcaca ttcttatacc
      121 cgaactcctt catgagtgcg ggtacgacct gttgctgata catgtcacga agtctcggct
      181 tatgcttcga ctcctcgctg ccctgatcta attgttgggg cgccgcgtcc ttcttcgccg
      241 tctttcgctc agatggtttt ccactgcggc ctttttctac cttcgccatt cctaagtcct
      301 cacctactca acagtctcat tcgatttctt gctgaagcgc acccgccggc catcctccaa
      361 tgtgcgaact cccacccgcg tcggcctttg agtgacgggg cagaagaaca tgacgttgga
      421 gatctggagg ggtgcttccc gctcaagaat cccgccctgc ttggcctttt gattcggctt
      481 cgtgtgccgc ttgatgatat tcagcttttc cacgatcacc ttaccggcgc cgagatccac
      541 tgagaggacc ttgccggtct tcccccgctc acgaccggaa atcacgacga ccgtgtcacc
      601 tttgcgaatt ctgctctttc gaagtgcctg cacagcgccc cccactcact cgcccctaca
      661 acacctcagg tgccagggag atgatcttca tgaatttctt ccaccgtagt tcacgcgcaa
      721 ccggaccgaa gatacgggtc ccgatcggct ctccatcctt attgatcaac acacaagcgt
      781 tccggtcgaa cttaatgtag gacccgtctt cacgccggac ttccttggtc gtccgcacaa
      841 tcaccgcgcg gctgacgtcg cccttcttca cgctcgcctg cggaatggct tccttgacgg
      901 ccacgacgac gatatcgccc aacgaggcat agcgccgtct tgttcccccg aagacatgga
      961 agcacatcgc ctgcttcgcc ccggagttat cggccacatc catgtatgta tagttctgaa
     1021 tcatgtgctg gatctttcct tacgtcccgc cacgtccgta attagtcggc ccgacctttt
     1081 tgaatgactt cgaccacacg ccaatgcttt tccttgctga tcggtcgagt ctcgaccatg
     1141 cgaacctgat ctccgatccg acacacattc tgctcgtcat gcgccttcat ctttgtgacc
     1201 cgtcgcagca cttttcgata gaccggatgg atcaccgatc gctcgacggc cacgacgacc
     1261 gtcttgttca tcttattact gagcacacga ccgacccact cacgtttttt cacagtcccg
     1321 tccgtcatag tcataaccct ttagccttcg cttctttctt tgacacctcg gtcatcaccg
     1381 tcttcagtct ggcgatatcc cgcttcgttt tccgaatctg catgggattt tcgagacgcc
     1441 cggtgccgaa ctgaaaacga agattgaaca gttcctgcac gagctgcttt tccttatccg
     1501 ccagctcagg cccagtcagt tgtcgcagct ctttcagatc caacgccata tcctcaactc
     1561 ctcactcaca cacaaacgct tagctgaatt ctccccgtac gaccagcctg gtagcaacag
     1621 gaagcttgtg ggacgcaagc cgaaaggcct cttttgcaat ctcgggcgtg actccgtcca
     1681 tctcatagag aatccggccg ggcttcacga cggcaaccca gtactcagga ttgcccttcc
     1741 ctttt
//
LOCUS       DATIFR010000020         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_968880, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000020 DATIFR010000000
VERSION     DATIFR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968880"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VLD60_00795"
     CDS             <1..928
                     /locus_tag="VLD60_00795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HSB43513.1"
                     /translation="TGVLFPLNSYENRVYEIGIEDEETGKPTAIVAKYYRPGRWSQET
                     IADEHGFVAALAEAEIPVVAHLPLRSHLKALPTLAKTVDGIFYAFFPKFRGREHAEIL
                     NDDRRWLGRSLARMHNVGEAFKAPRRMRLTPETYGHNSLAFILTQSFLPPDLKQSIES
                     HLQKAIELVEPHFKRDWSPIAVHGDCHPANILWNKDGPHLLDFDDMVVAPAVQDVWML
                     FNGTEDEKSEQKKVFLEGYEIFREFDDASLILAEPLRTLRMIRHAAWIGQRYEEPAFQ
                     RAFPYYRERRYWEEYLQSIKEQISLLQELSWS"
     gene            complement(929..2113)
                     /locus_tag="VLD60_00800"
     CDS             complement(929..2113)
                     /locus_tag="VLD60_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB43514.1"
                     /translation="MPHLRPRYLAAILKKDLSWSPVVSVLGMRQVGKTTLLKQLGTSY
                     VTLDDDDVLKRFEQGIWTEIESAPAPLVVDEAQKSPGLFDRVKLIVDRRKRPGQFLLT
                     GSVRFLSRQQIRESLTGRTSILELLPLTVAETHSRPLTDFIKEATRFPPETVLKKCAM
                     RRRFPRKGIEDYVSLGGMPGVCFKRDPLVRRRMQESHLETLLMRDLQFLVKTRIPFVK
                     LRAILSTMAQIQGQSLSLSDLGRKVQLSTPTIIQLIRAFENLFIVRPHGKSWFFADCG
                     MASFLGAALNENALFHMERFVYQELYAQWNYLHASSFRFHPYLTRGGVRIPFVIDVQD
                     SPTLAIVVDATDGASEKSLKSLTWFAKKHKKTVASVVLHRGSKAYLASTSAICLPYDW
                     IV"
     gene            2559..2726
                     /locus_tag="VLD60_00805"
     CDS             2559..2726
                     /locus_tag="VLD60_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43515.1"
                     /translation="MGLFLGQFSAAGPIFAGIVTSVGRQGAEMAILDSGNQSLIAGGP
                     ILFLENNGKNK"
     gene            complement(2763..3053)
                     /locus_tag="VLD60_00810"
     CDS             complement(2763..3053)
                     /locus_tag="VLD60_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB43516.1"
                     /translation="MRLPARRIETGSEKKIDEVSLSIKHYARGGKQRSFALGERIKKL
                     RKERVLTQENLARAAGISRMQMNRIESDVSKPGLETMMRLADALGVPLRDTV"
BASE COUNT          717 a          867 c          864 g          678 t
ORIGIN      
        1 caccggcgtc ctcttccctc tcaacagcta tgaaaaccgc gtctatgaga tcgggatcga
       61 ggacgaggag acgggaaaac ccacggccat cgtggccaag tattaccgcc ccggccgctg
      121 gagccaggag acgatcgccg acgagcatgg attcgtggcg gccctcgcgg aggcggaaat
      181 cccggtggtc gcgcatctgc cgctccggag ccatctgaag gccctcccga cgctggccaa
      241 gacagtcgac ggaatcttct acgccttctt ccccaagttt cgcggccgcg agcacgcgga
      301 aatcttgaac gacgaccgcc gctggctcgg ccggagcctg gcgcgcatgc acaacgtcgg
      361 cgaggccttc aaggcaccgc gccggatgcg gctgactccc gagacctacg gccacaacag
      421 cctggcattc atcctgacgc agtcctttct tccgccggat ctgaagcaga gcatcgagtc
      481 gcaccttcag aaggcgatcg aactggtcga gccgcatttc aaaagggatt ggagtcccat
      541 cgccgtccac ggcgactgcc acccagccaa catcctctgg aacaaggacg gtccgcactt
      601 gctcgacttc gacgacatgg tcgtcgcccc ggcggtccag gacgtttgga tgctcttcaa
      661 cggcacggaa gacgagaaat ccgagcagaa gaaggtgttt ttggagggct acgagatttt
      721 ccgggagttc gacgacgcaa gcctcattct cgccgaaccg ctgcgcacgc tccggatgat
      781 ccgccacgcg gcctggatcg gccaacgcta cgaggaaccg gcctttcaac gggcgtttcc
      841 gtattaccgg gagaggcggt attgggagga gtacctgcag tcgatcaagg agcagatttc
      901 tctgcttcag gaattgtctt ggtcctgatt aaacgatcca atcataagga aggcaaatgg
      961 cggatgtcga agccaagtag gcttttgaac ctcgatggag gacgacggag gcgacggttt
     1021 ttttgtgttt ttttgcaaac cacgtgaggc tcttgagcga tttttcgctg gctccatcgg
     1081 tcgcgtcgac gacaatcgcc agggtcggag aatcctggac gtcgatgacg aaggggatgc
     1141 ggacgccccc gcgggtcaaa tagggatgaa accggaagct cgatgcgtgg aggtagttcc
     1201 attgggcgta aagctcctga taaacaaacc gctccatgtg aaaaagggcg ttttcgttga
     1261 gtgcggctcc cagaaagctc gccattccgc aatcggcgaa aaaccagctc ttcccatgag
     1321 gacggacgat aaaaagattc tcgaaggccc ggatcagctg gataatcgtg ggcgttgaaa
     1381 gctgaacttt ccttcctagg tcggaaaggg acagggactg cccctgaatt tgagccatgg
     1441 tggaaagaat cgcccggagc ttcacgaagg gtatgcgggt tttgaccaga aactgcaggt
     1501 cgcgcatcag aagggtttcc agatggctct cctgcattct ccggcgcacg agagggtccc
     1561 ttttaaaaca gacccccggc atgcctccaa gactcacata atcctcaatt cccttccttg
     1621 gaaaccgtct gcgcatggcg catttcttca gaacggtttc gggagggaat cgtgtcgcct
     1681 ctttgatgaa gtccgttaac ggccgcgaat gcgtctccgc caccgtcaag ggaagcaatt
     1741 ccagaatgga ggtccggccc gtcagggatt cgcggatctg ttgtctcgac aggaaacgga
     1801 ccgaaccggt gaggaggaac tggccgggtc tttttctccg gtcgacgatc aatttgaccc
     1861 ggtcaaaaag cccggggctt ttctgcgcct catcgacgac caggggagcc ggagccgact
     1921 cgatttccgt ccagatcccc tgctcgaacc gtttgagaac atcgtcgtcg tccaatgtaa
     1981 cgtagctcgt cccgagttgt ttcaagaggg tcgtctttcc gacttgccgc attcccagga
     2041 cggaaacgac gggagaccat gagaggtctt ttttcagaat cgccgcgaga taacgaggtc
     2101 gaagatgggg catgagccga cgatatcatg tttatgctat atgtaaatta gttatttata
     2161 cattacataa acgtgaatac cgcagtcgcc gcggcccaac tggcggcggt attgggagga
     2221 gtacctgcag tcgatcaagg agcagatttc gctgcttcaa gagtcggcgt gggcttgaag
     2281 cgaatccaaa gaatgagaca tcttttactt tgaggtgtca gcagatcccg ccacgcgaaa
     2341 tccagcgaac ggaagttgtt tgtccgggta agagctaatt cggaatgggt tgcgcagctg
     2401 atccggatta tatgccgcaa aaaacctcct cgcagaccat agtgatcctt ttgatttgga
     2461 tttctggagg taaattcaaa aacattgcct gtcatgtcat ataggccaaa accattaggg
     2521 ggatatgagc cgacctcaca cgttgtggag gaattatagt gggcctcttc ctcggtcagt
     2581 tctccgctgc aggtcccata ttcgccggga tcgtcaccag cgtagggaga caaggggctg
     2641 aaatggccat tttggactcc ggcaatcagt ctcttatcgc cggcggcccg attttgtttc
     2701 tggaaaacaa cggtaaaaac aaatagttat gccaagagca atgcaaccaa tcggcatgat
     2761 tttcaaaccg tatccctcaa aggaaccccc agcgcatctg ccagacgcat catggtctca
     2821 agccccggct tggagacatc cgactcgatg cgattcattt gcatgcggct gatgccggcc
     2881 gcccgcgcca gattctcctg ggtcaatact ctctctttcc tcaacttctt gattcgttcg
     2941 cccaaggcaa aactcctctg cttccctccc cgcgcgtaat gcttgatcga gagactgact
     3001 tcatcgatct tcttctccga tccggtttca atccttcttg ccggaagcct catggaacct
     3061 cgacccataa gtttatggat aacatgccct tagtatcgta gaatacctcg aaaatcgcac
     3121 ggagga
//
LOCUS       DATIFR010000021         9213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_968967, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000021 DATIFR010000000
VERSION     DATIFR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968967"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VLD60_00815"
     CDS             complement(<1..627)
                     /locus_tag="VLD60_00815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSB43517.1"
                     /translation="MALIVQKYGGTSVGTIERIHRVADRVEQAAKGGNQVVVVLSAMS
                     GETDRLLKLAHEITAIPDERELDMLLSTGERVTIALLAMDLRGRGLDARSFTGRQVGI
                     MTDSAHTKARITRVTAERIRESLRKGVIPVVAGFQGINEQSDVTTLGRGGSDLTAVAL
                     AGALKADRCVIFTDVDGVYTTDPNIVPSARRIDKISYEEMLEMASLGAK"
     gene            complement(633..1874)
                     /locus_tag="VLD60_00820"
     CDS             complement(633..1874)
                     /locus_tag="VLD60_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43518.1"
                     /translation="MKYVILHAEGMADHPRQELAGKTPLQAASTPHLDRLAQGSELGL
                     LTVALENGRHGSGLTGTSILGYEPKKYYQGPGPLEAASLGVTVGEHDVVYRCTMVTLK
                     AEQPSGAKGGGSDLKKLGPHLMMEDATAGLISTEEARELIDAVNEQLGSEMIQFYPGA
                     GHRHLMVWVDGKSRAVCTDPQSLVSRSIVDGLPKGDGADILRKLMDASLLILRDHPVN
                     DDRRDAGSKPANCLWLWGEGRAIPWPSLTERFDISGVVISSSDVHRGLGLVAGLEAVD
                     PARLSGADLRSRAIVALDELKKKDFAYVHLELPDEVVYDPDVKAKVREIEAVDKEFVG
                     PFLDGLAKLGPHRLVTISDPGSVHQGKMAEGQWLYAYQDSAAKSASGSGRRFTEADAK
                     ASATPPRDATKFVVRLFTKGS"
     gene            complement(1899..2957)
                     /gene="thrC"
                     /locus_tag="VLD60_00825"
     CDS             complement(1899..2957)
                     /gene="thrC"
                     /locus_tag="VLD60_00825"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSB43519.1"
                     /translation="MNRWRGVIEEYRKFLPVTERTPVITLGEGNTPLIRAARLAKKIA
                     PGVDLYLKFEGANPTGSFKDRGMTMAISKAVEAGARAVICASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQIDGNFDQALSIVKDLSATYHIELVNSLNP
                     YRIEGQKTAAMEVCDQLGDAPAVHALPVGNAGNITAYWKGYKEYRSANQTTRLPRMVG
                     FQAAGAAPIVLGRVVENPQTVATAIRIGNPASWQAAVTAMKESSGLIDSVTDEEILQA
                     YAAVAATEGVFCEPASAASVAGIMKLSRQGGLREGETVVCTLTGHGLKDADTAISVSV
                     KPKTVQATREDVARLLNV"
     gene            complement(2962..4275)
                     /locus_tag="VLD60_00830"
     CDS             complement(2962..4275)
                     /locus_tag="VLD60_00830"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSB43520.1"
                     /translation="MISRVGVGVIGFGTVGTGVAKILLENAALIRRRVGVPIELVRIA
                     DLDVTRDRGITLPAGVLTNDAKAVLDDPNIHIVVELIGGYDFAKRLLLDAIAAGKEVV
                     TANKALLALHGEEIFAAASRRGVDVGFEASVGGGIPVIQAIKEGLAANTIQSIYGIIN
                     GTSNYILSLMTNEGKGFDETLVEAQRAGYAEADPTFDVAGTDSAHKLAILANLAFGTP
                     INFKELYTEGITHVTKHDIAYARELGYTIKLLGIAKLVEGDVEARVHPTMLPADSPLA
                     KIEGVYNAIQLVGDAVGDVVLVGRGAGDMAAGSAVVSDIIASARNLLKGGAGRVPPAS
                     FQPDERRPLRMRPMEEIRSLYYLRFMVIDRPGVLSQIAGELGRCGISISSMLQKGRRE
                     GQTVPIVIKTHMARERDVQSALREINRMAFMSEPATLIRVEGKDE"
     gene            complement(4272..5468)
                     /gene="alaC"
                     /locus_tag="VLD60_00835"
     CDS             complement(4272..5468)
                     /gene="alaC"
                     /locus_tag="VLD60_00835"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HSB43521.1"
                     /translation="MGMADNFYRLKRLPPYVFAQVQSLKLEARHRGEDIIDFGMGNPD
                     QPTPSHIVEKMIEAARKGKNHRYSASRGITKLRHAIAGWYKRNYDVDLDPETETIVTI
                     GSKEGLAHLSLAMIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRPDSDFFDDLQR
                     VYRQTFPRPRILVINFPHNPTTAIVDLEFFKKIVAFAKEHNVIVIHDLAYADLVFDGY
                     KAPSFLQVPGAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKIKSYLDYGIFQ
                     PLQIASVIALNGPQDCVKETVQRYQKRRDVLVGGLNRIGWNVSKPLATMFVWAKIPLP
                     YRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEYVRFALVENEHRTRQAVRGIRKALK
                     LEGGEE"
     gene            5748..6461
                     /locus_tag="VLD60_00840"
     CDS             5748..6461
                     /locus_tag="VLD60_00840"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HSB43522.1"
                     /translation="MPRLGVNVDHVATLRQTRGGNDPDPLAAAVLVELAGADGIVVHL
                     REDRRHIQDRDLQLLRELIRTKLDLEMAADETMAKIALTVKPDMVTLVPERRQELTTE
                     GGLDVATHKDRIQQIVEMLHNSGIPVSLFIEPDLAQIKAAHKISADFVELHTGKYANA
                     KRSKEADAEFEAITQAAKLAYKLGMGVNAGHGLNYHNVKRLTHIPEIVEYNIGHSIVA
                     RAVMVGLDQAVREMKALLS"
     gene            6540..8090
                     /locus_tag="VLD60_00845"
     CDS             6540..8090
                     /locus_tag="VLD60_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSB43523.1"
                     /translation="MNIVTGTQMQALDRRTITEAQVPSLVLMERAGGGVADFIQHRFS
                     PLRGKTATILCGKGNNGGDGFVVARLLRQRHAKVTVLLLAPGGDLSRDAAMMYRRWLR
                     VAGKSSTRQFRSSEQAGAMLASSDFIVDALLGTGLSTEVTGVYRDAIRLMNSAGRPVI
                     AVDMPSGIHADNGALLGEAVRATATITLGLPKLGLYLGAGIDHAGTIQVVDIGIPPAY
                     VEAVNSRAIVVTNELVAKTLPARPASAHKGTFGHAGIIAGSVGKTGAAALAARAALRI
                     GAGLVTVGTPSSVNDVLEAKLLEAMTVSLPETKARTLARSGLDRVLAFMQTRTALAIG
                     PGLSTHPETVELVQSLMKHLDRPSVLDADALNALASRASLLTECKIPPILTPHPGEMA
                     RLEVDATSQSVNADRLGTARRFARERGVFVVLKGARTVIARPDGLLAICPTGNPGMAT
                     AGTGDVLTGMIVGLLAQAVPAWEAACAATYLHGLAGDLAAQQLGQAGMLAGDLIDHIP
                     YALQAINR"
     gene            8065..8685
                     /gene="tsaE"
                     /locus_tag="VLD60_00850"
     CDS             8065..8685
                     /gene="tsaE"
                     /locus_tag="VLD60_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSB43524.1"
                     /translation="MRSRRSTASRYVHARSVGATRPVSVRSNHVWEVASPSPVHTDRL
                     GHAIGLSLQGGETLALFGPLGAGKTSLVRGIAAGLGAPASAVSSPTFVLIHEYRGRLP
                     LAHVDLYRVNSTREAESTGLQEYFSGSTVAAIEWADKGLSILPQDRLEIQLRHQAVGS
                     RTIRLSATGPTSAALMAEVRRRYARTAKRRVTTRPSQPQPKRKASS"
     gene            8682..9029
                     /locus_tag="VLD60_00855"
     CDS             8682..9029
                     /locus_tag="VLD60_00855"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HSB43525.1"
                     /translation="MIRLILVGTLLLLCLAFFLQNQEQEVTLRYFFGLFEASTPIYKP
                     ILAGFAVGLLVSGILLFPPWVKGRIEVRRKTKALQEAEVDLERLRHSLEKFTGRTSPP
                     VPGDVPQRDRSDE"
     gene            9022..>9213
                     /locus_tag="VLD60_00860"
     CDS             9022..>9213
                     /locus_tag="VLD60_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43526.1"
                     /translation="MSDHDATPLMRQYREIKRGYPEAILLFRVGDFYEMFYEDAQAAS
                     KLLSIALTSRDKSSTDPVPL"
BASE COUNT         1824 a         2924 c         2583 g         1882 t
ORIGIN      
        1 cttggcgccc agactcgcca tctccaacat ttcctcgtag gaaatcttgt cgatccgcct
       61 ggccgacggc acgatgttcg ggtccgtcgt atagaccccg tcgacatcgg tgaaaatgac
      121 gcagcgatcc gccttcaagg caccggctag cgccaccgcc gtcaggtcgg aacccccgcg
      181 acccaacgtc gtcacatcag actgttcatt aatcccttgg aagccggcga ccaccggaat
      241 aacccctttg cgcaacgatt cacgaatccg ctccgcagtc actcgggtaa tccgcgcctt
      301 ggtatgggcg ctgtcggtca tgattccgac ctgacgtccc gtaaaggaac gagcatccag
      361 tccccgacct cgcagatcca tggccaggag tgcgatcgtc acccgctcgc cggtcgagag
      421 gagcatgtcg agttcgcgtt catcaggaat cgcggtgatc tcgtgggcca gcttcagcag
      481 gcgatcggtc tcgccgctca tcgccgagag gacaacgacg acttggttgc ctcctttggc
      541 cgcctgctcc acacgatccg ccacacggtg aatccgttcg atcgtgccga cagaggttcc
      601 tccatatttt tggacgatca atgccacgac cgttacgacc cttttgtgaa gagacgtacc
      661 acgaacttcg tggcatctcg gggcggggtg gccgatgcct tggcatcggc ttcggtaaac
      721 cgacgaccag atcctgaagc ggattttgcc gcgctatcct ggtaggcgta aagccattgc
      781 ccttccgcca tctttccctg atgcacgctg ccgggatcgc tgatcgtcac cagacgatga
      841 ggccccagtt tcgcaagccc gtcgaggaac ggcccgacga actccttgtc gaccgcttca
      901 atctcgcgca ccttggcttt cacgtcagga tcgtagacga cctcgtccgg cagctccaga
      961 tgcacatagg cgaagtcctt cttcttcaac tcgtcgagcg ccacgatggc ccgactccga
     1021 agatcagcgc cactcagacg agctgggtca accgcctcaa gcccggccac caaaccgagc
     1081 ccgcggtgga catcgctcga agaaatcacc acaccggaaa tgtcaaatcg ctctgtcaga
     1141 ctcggccagg gaatcgctcg cccttcgccc cacagccaca gacagttggc tggtttgctc
     1201 ccggcatcgc gccggtcatc attcaccgga tggtcgcgca gaatcagcaa tgaggcatcc
     1261 atcagcttgc ggaggatatc ggcgccgtct cccttcggca acccatccac gatcgatcgc
     1321 gagaccagtg attgcgggtc cgtgcagacg gctcgcgatt tcccgtcgac ccagaccatt
     1381 aagtggcgat ggcccgcacc gggatagaat tgaatcatct cggacccgag ctgctcattc
     1441 acggcgtcga tcaactcgcg ggcttcctcg gtgctaatca gcccggctgt cgcatcctcc
     1501 atcatgaggt ggggaccgag tttcttgagg tctgagcctc cgcctttggc gccggatggc
     1561 tgttcggcct tcagcgtcac catcgtgcaa cggtagacca catcgtgctc gccgacggtc
     1621 acgcccaggc tcgccgcctc caacggcccg ggcccctggt aatatttctt cggttcgtac
     1681 ccgagaatcg acgtgccggt gagaccgctg ccgtgccgcc cgttttccag cgcaacagtg
     1741 agaaggccca gctcgctgcc ttgagccaaa cgatcgaggt gaggagtcga cgccgcctga
     1801 agaggggtct tgcccgccaa ttcctgccgg ggatggtcgg ccatcccctc agcatggaga
     1861 atcacatatt tcatcgaacg cacactcgca tcataccctt acacgttgag gagacgagcc
     1921 acatcctccc tggtcgcttg aacagttttc ggtttcaccg acacgctgat tgcggtatcg
     1981 gcgtctttca gaccatggcc ggtcaacgta cagacaaccg tttcgccttc ccgtagtccc
     2041 ccctgccggc tgagcttcat gatcccagcc acggatgcag cagaggccgg ctcgcaaaag
     2101 accccttccg tcgccgccac tgcggcgtat gcctgcaaga tttcctcgtc ggtcaccgaa
     2161 tcgatcaaac cggacgattc tttcatcgcc gtgacggccg cttgccaact cgcgggattg
     2221 ccgattcgaa tcgccgtggc gacagtttgt ggattctcca cgacccgccc gagcacgatc
     2281 ggagcggccc cggctgcctg aaagccgacc atgcgcggca atcgcgtcgt ttgattggcc
     2341 gaccgatatt ccttataacc cttccaatag gccgtgatgt tgccggcatt cccgacggga
     2401 agcgcgtgca cggccggcgc atcaccaagc tggtcgcaca cttccatcgc cgccgttttc
     2461 tgcccttcga tcctgtaggg gttgagggaa ttcaccaact cgatgtggta cgtcgcggac
     2521 aggtctttca caatcgagag ggcttggtcg aagttgccgt cgatctggat gaccgtcgcc
     2581 tggtgcatca tggcctgcga gagtttcccc atagcgatct ttcccgcagg gatcagcacg
     2641 tatacggcca gccccgccct cgcgccataa gccgcagcgg acgccgacgt gttgcctgtg
     2701 gatgcgcaga tcaccgcacg agcgccggcc tcaacagctt tcgaaatcgc catcgtcatc
     2761 ccgcggtcct tgaacgaacc tgtgggattg gcgccctcga acttcaaata gagatcgacg
     2821 ccgggagcga ttttcttcgc cagtctcgcg gccctgatga gcggggtgtt gccttcgcca
     2881 agcgtgatca ccggcgtgcg ttccgtgaca ggcaggaact tgcgatactc ttcgatgacc
     2941 ccacgccagc ggttcatgac atcactcgtc cttgccctcg acacgtatta gggtcgccgg
     3001 ctcggacatg aacgccatac ggttgatttc gcggagggca gattggacgt cgcgctccct
     3061 cgccatatgt gtcttgatga caatggggac ggtttgtccc tcccgccgcc ctttctgcaa
     3121 catcgacgag atgctgatgc cacagcggcc caattctcct gcgatctgag acagaactcc
     3181 gggacgatcg atcaccatga atcgcaggta gtagagggag cgaatctctt ccatggggcg
     3241 catgcgaagc ggacgtcgtt catccggctg gaacgacgcc ggaggcaccc gtcccgctcc
     3301 gcccttgagc agattgcggg cgctggcgat gatgtcgctg acgacggcgc tccccgctgc
     3361 catatcgccg gccccacggc ctaccaacac gacgtctccg accgcatcac cgaccaattg
     3421 aatcgcgtta tacacccctt caatcttcgc cagaggagaa tctgcgggaa gcatcgtggg
     3481 atgaacgcgc gcctcgacgt ccccctcgac cagcttcgcg atccccaaca gcttgatcgt
     3541 atagccaagt tcacgagcgt aggcgatgtc gtgtttggtg acgtgagtga tgccttctgt
     3601 gtacagctcc ttgaaattaa tcggcgtgcc gaacgctaaa ttggccagga tcgccagctt
     3661 gtgcgcggaa tcagtgccag ccacatcgaa cgttggatcg gcctccgcat agccggcacg
     3721 ctgagcctcc acgagcgtct catcaaatcc tttcccctca ttggtcatga gggaaagaat
     3781 gtagttggac gtcccgttga tgatgccgta gatggattga atcgtgttgg ctgcgaggcc
     3841 ttctttgatg gcctgaatga cggggatccc cccgcccacg ctggcttcaa acccgacgtc
     3901 gacgcccctg cgggaagccg ccgcaaagat ttcttccccg tgcagagcca acaaagcttt
     3961 attggcggtc acaacttctt ttcccgccgc aattgcatcc agcaggagac gcttcgcgaa
     4021 gtcgtagcct ccgatcaact ccacaacgat gtggatgttc ggatcatcca acaccgcttt
     4081 ggcatcgttc gtcagcaccc cggctggaag ggtaatgccg cggtcgcgcg tcacatcgag
     4141 atccgcaatg cgaacgagtt cgatcggcac accgacccgc cgacggatca gcgccgcgtt
     4201 ttccagaaga atcttggcaa cccccgttcc gaccgtgccg aagccgatca ccccgacgcc
     4261 cacgcgcgag atcattcctc tcccccttcg agcttcagcg cctttcgaat cccgcggacc
     4321 gcctgcctag tccgatgctc gttttccacc aaggcgaaac ggacatactc atcgccaccc
     4381 tcgccgaatc cgatcccggg cgacaccgca accttcgctt cccgaagcaa gagtttggaa
     4441 aattcgagtg agcccatgtg ccgatagggc aatggaattt tggcccagac gaacatggtg
     4501 gccaacggct tgctgacatt ccacccgatg cgattgaggc ccccgacgag gacatcgcgc
     4561 cgtttctgat accgctggac ggtttccttc acgcaatctt gcgggccgtt cagcgcgatg
     4621 acgctcgcga tctgcagcgg ctgaaagatc ccgtagtcga gataactttt gatcttggcc
     4681 aacgcgccaa cgacttctcg gttgccgaca cagaacccta cacgccagcc tggcatgtta
     4741 taggccttgg acagggtgta aaactccact ccgacatctt tggcgccggg cacctgaaga
     4801 aagctgggcg ccttgtaccc gtcaaagacc aagtcggcat aggccaaatc atggatgacg
     4861 atcacattat gctctttggc aaaggcgacg attttcttaa aaaactccag atccacgatt
     4921 gccgttgtgg gattgtgtgg aaagttgatg accagaatcc tcggccgggg aaaggtctga
     4981 cgatagaccc gctggagatc atcgaaaaaa tcgctgtcag gacgcaactc gatcccgcga
     5041 acttccccgc ccgcaataat gaaactgtac atatggatcg ggtacgttgg agtcggtgtc
     5101 agcacgacat cgcctggccc gatcatggcc aaggacaagt gggccagacc ttcttttgac
     5161 ccgattgtaa caattgtttc ggtttcgggg tcgagatcga cgtcgtaatt tcgcttgtac
     5221 cagccggcga tggcatgacg cagcttcgtg atgccacgcg aagcggagta gcggtggttc
     5281 tttcccttcc gcgcagcttc gatcatcttc tcgacgatat ggctcggcgt cggttggtcc
     5341 ggattcccca tcccgaagtc gataatgtct tcgccgcggt ggcgcgcctc cagcttcagg
     5401 ctctgaacct gcgcgaagac atacggcgga agtcgcttga gtcgataaaa gttatcggcc
     5461 attcccattg cagttgcctg tcaagtttac ccttgccgga agggagaaac cagctcccgg
     5521 tttcgggaag gcctattcta atggcgcgac tagagtggag tcaaattatc ttgtgattct
     5581 gtggggttct gcatccgaca cctatcgagt aaggtagttc ggccagggcg aaaccgttcc
     5641 atcccagtcc ttgcccgtga cgctggtttc tgttacaaca gcggcgactt ctctgtttca
     5701 tcgcctgagt tcaactctcc ctgttagccc ggatccctgg aggccccgtg cccagacttg
     5761 gtgtcaatgt cgatcatgtc gcgacgcttc gccagacacg cggagggaac gacccggacc
     5821 ccctcgccgc cgccgtgttg gtagaactgg ccggcgccga cggcatcgtc gtgcacctca
     5881 gagaggatcg ccgtcatatt caagatcgcg acctgcagct cttgcgggag ttaatccgga
     5941 ccaagctgga tctggaaatg gccgccgacg agacgatggc caaaatcgca ctgaccgtga
     6001 agcccgatat ggtgacgctg gttcccgaac ggcggcagga actcaccacg gaagggggcc
     6061 tcgacgtcgc cacgcacaaa gaccgcattc agcagatcgt cgaaatgctg cacaacagcg
     6121 gcattcccgt cagtcttttt atcgaaccgg acttggccca gatcaaggcg gcacataaga
     6181 tctccgccga ttttgtcgaa ctccataccg gcaagtacgc caatgcgaaa cgatcgaaag
     6241 aggccgatgc tgaattcgag gcgatcacgc aggcggccaa gctggcctat aaattgggga
     6301 tgggcgtcaa tgcggggcat ggactcaact accacaacgt gaaacgcctc acccacattc
     6361 cggaaatcgt ggaatacaac atcggccaca gcatcgtcgc ccgcgccgtc atggtcggtc
     6421 tcgatcaggc cgtacgagaa atgaaggcgc tgctcagttg atcgtgacct tcgctcagct
     6481 caccctcggt cggcaaagac cgtccgggac gatccgacaa tgaccgcaag ggccaccaca
     6541 tgaacatcgt gaccgggact cagatgcagg ccctggaccg ccggacgatc acggaggctc
     6601 aggttcccag cttggtcctg atggaacggg ccgggggcgg agttgctgat ttcatccagc
     6661 atcggttctc gccgcttcgc ggcaagactg ccacgattct ctgcggcaaa ggcaacaacg
     6721 gcggcgacgg gtttgttgtt gcgcgcttgc ttcgccagcg ccacgcgaag gttaccgtgc
     6781 tgctcctggc accggggggt gacctcagtc gcgacgctgc catgatgtat cgtcgctggc
     6841 tgcgagttgc gggaaagtcc tccacaaggc agttccgatc ttccgaacaa gccggtgcga
     6901 tgctggcctc cagcgatttc atcgtcgatg cccttctcgg cactggcttg tcgaccgaag
     6961 tcaccggtgt ctatcgagat gccatcaggc ttatgaacag cgcgggccgg ccggtcatag
     7021 cggtggatat gccctccggc atccatgccg acaacggagc cctgctgggc gaagccgttc
     7081 gtgccaccgc aaccatcacg cttggcctcc ccaagctcgg cctatacctt ggagccggca
     7141 tcgatcacgc cgggaccatc caagtcgtcg acatcggtat ccctccagcc tatgtcgagg
     7201 cggtgaacag ccgggccatt gtcgtcacga acgagttggt cgcgaaaacc ttgccggctc
     7261 gcccggcctc tgcgcacaaa ggcactttcg gtcatgccgg catcatcgcc ggttcggtcg
     7321 gcaagaccgg tgcggcggca ctcgcggcac gggccgcctt gcgaattggc gccggcctcg
     7381 tgacggtggg aactcccagc agcgtcaatg atgtactgga agccaagctc ctcgaagcga
     7441 tgaccgtttc gctccccgag accaaggctc gcaccctggc ccgttccggc ctggatcgcg
     7501 ttcttgcttt catgcaaacc agaacggctc ttgctatcgg cccagggttg tcgacgcatc
     7561 cggaaacagt cgagctcgtc caatccttga tgaaacattt ggatcgaccg tccgtgctgg
     7621 atgcagacgc actcaatgcg ttggccagcc gtgcttctct gctcacggaa tgcaaaattc
     7681 ctcctatatt gacgccgcat ccaggagaaa tggcgcggct tgaggtcgat gcgacctcac
     7741 aaagcgtgaa tgccgatcgc ctcggcacgg ccagacgatt tgcccgtgaa cggggggtct
     7801 tcgtcgtgct caaaggagcg cgcaccgtga ttgcgcgccc cgatggcctc ttggcaatct
     7861 gcccgaccgg caaccccgga atggcaacag ccggaacggg ggacgtctta accggtatga
     7921 ttgtcggatt gctggcccaa gcagtcccgg cctgggaagc cgcttgtgct gcaacgtatc
     7981 ttcatggatt ggccggcgac ttggcggccc aacaactcgg gcaggccggc atgctcgccg
     8041 gtgatcttat cgaccacatt ccctatgcgc tccaggcgat caaccgctag tcgttacgtt
     8101 cacgctcggt cggtcggggc aacccgtccc gtcagcgtac gatcgaatca cgtctgggaa
     8161 gtggcctccc cctcgcccgt ccatacggat cgtcttggtc acgccatagg cctgtcgctg
     8221 cagggagggg aaacgctcgc gctcttcggt cccctgggag cggggaaaac atctctggtc
     8281 cgaggaattg ccgccggttt aggcgccccc gctagcgctg tgagcagccc aaccttcgta
     8341 ctcatccatg aatatcgagg gcggcttccg ctggcccacg tcgatctgta tcgtgtcaat
     8401 tcaacccgcg aagccgagtc aacgggattg caggaatatt tttcaggttc gacggtcgcc
     8461 gcgatcgaat gggccgacaa gggcctctcg atcctgccgc aggaccggct ggagattcag
     8521 ttgcgccacc aggcggttgg gagtagaacg attcggctgt cggcgaccgg accgacttcg
     8581 gctgccttga tggccgaggt cagacggcga tacgctcgca ccgcgaagcg acgcgttacg
     8641 acgcggccct cgcagccaca gcccaagcga aaggcgtcgt catgattcgg ctcattctgg
     8701 tcgggacact ccttctctta tgcctggcct tctttcttca gaaccaggaa caagaggtca
     8761 cgcttcgcta ctttttcggc ttgttcgaag cctcgacgcc gatttataaa ccgatcttgg
     8821 ctggctttgc cgttgggctc ttggtctccg gtattctctt gttccctccg tgggtgaaag
     8881 ggcgcatcga ggtgcgcagg aaaaccaaag ccctccagga agccgaggtc gatctcgaac
     8941 gattgcgcca ctcgcttgag aaattcaccg gacgcacgtc acctccggtg ccgggtgatg
     9001 tcccgcaacg agaccggtca gatgagtgat cacgacgcta cgccgctcat gcggcagtat
     9061 cgggaaatca aacgcggcta tcctgaagcc attctgttgt ttcgggttgg cgacttctac
     9121 gaaatgtttt atgaggatgc gcaagccgcc tccaaactgc tctccatcgc cctgacgtca
     9181 cgagacaagt cgagcacgga tccggtgccg ctc
//
LOCUS       DATIFR010000022        36490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1543645, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000022 DATIFR010000000
VERSION     DATIFR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36490
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543645"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VLD60_00865"
     CDS             <1..616
                     /locus_tag="VLD60_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43527.1"
                     /translation="IRGRLTYTSAEKDSIKLWTPEGEKEFAVKRNRPKFTMFQDGALI
                     TVQLNDKGEVIDVHRQLELELAVAPAPHIEPGFRIKLEGVVADIKSGQVFVQTPGGQY
                     SLNAKTAPQDVKVGDKLTLWVSGNNVAIDHHAKGKEGVHRLITGSLTYASGDMKEIKL
                     MTPEGERTFVVQHGESKLSGMKEGTPITVELNEAGSVVEIRKAG"
     gene            1073..1570
                     /locus_tag="VLD60_00870"
     CDS             1073..1570
                     /locus_tag="VLD60_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HSB43528.1"
                     /translation="MSTSHLPRDKKDRSVRARKHDQYKAKGKLQEPSVCRECKALYHK
                     GRWMWGPVPSGSHDVVCPACERIRDGSPSGVLLLTGEFVAKHRDEVLGLARNEETRVK
                     SEHPLARIIKIENQTEAPEGVVITTTDPHLARRIGEALHHAHHGTFTCRYEENEDLLR
                     ANWQS"
     gene            1622..1849
                     /locus_tag="VLD60_00875"
     CDS             1622..1849
                     /locus_tag="VLD60_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43529.1"
                     /translation="MAISSWRIKATQKWGEHARWLSGEGPFAVIAPCREFAFSLWDTR
                     EAADKAKAHLDQMGCGGGCNPLLHKVVDLSE"
     gene            1976..2377
                     /locus_tag="VLD60_00880"
     CDS             1976..2377
                     /locus_tag="VLD60_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43530.1"
                     /translation="MFMYLLVLPIVMGFPLSGFAQFVERGEYIVVAKGTKLCANMILE
                     QSKGVLDPGKVFWRVTSNVRANGTQTIAAFARETVYDRKSGETTIKESRYDEDYATFF
                     KRDPEHKGMLLMVSPQDGRVQAIVEVCQKGR"
     gene            2906..3235
                     /locus_tag="VLD60_00885"
     CDS             2906..3235
                     /locus_tag="VLD60_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43531.1"
                     /translation="MRLLTIGMTGLVFVGMTTLTMANPSMLPKHPGYPSGGDYANDKG
                     QQNLSYNQSMVEAAKSGDTNMAPTLIDPNNARLVEHQGAGQLPIVQGPNIKIEPPVKE
                     GTRMPVK"
     gene            3547..4203
                     /locus_tag="VLD60_00890"
     CDS             3547..4203
                     /locus_tag="VLD60_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB43532.1"
                     /translation="MRHISLFTTSRGAWVRITGLSMLFLLVGAPVVSAEPTATESVNR
                     VITQVMSILDDPALKQPARSAERRQAIEDVVKQHVNYEEMARRALGAPWMEINSLERE
                     EFVGLFVGLLRDMLAGRINHYSLKQVVYLSEYREGNVAEVRTAWIGGKVDTSFDVRLA
                     KKAGDWLMYDAVIDGASIVENYRAQFSRIIRDVSYEGLVSRMKQKTLFVKAFEYAAVR
                     "
     gene            4368..4709
                     /locus_tag="VLD60_00895"
     CDS             4368..4709
                     /locus_tag="VLD60_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSB43533.1"
                     /translation="MDKKSMVIGAVVIGTIVVVGGMVWSAEKEKHKEADQTQVIAMAQ
                     AAKVTIDQAIKTALDNFPGKVIEAELEKKHDKAVWEVEILTAEQVIKVVHVDADSGSV
                     IVTEEKPSEKK"
     gene            4830..5729
                     /locus_tag="VLD60_00900"
     CDS             4830..5729
                     /locus_tag="VLD60_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43534.1"
                     /translation="MQRRYEFIDLIVALGLFATIVAGGLLFMAANGTLSLSLSRQSER
                     EQPIGPSNGTQWLQPVLGQAILDQNLLDLHYEKVTPGAVTQLNEVTGEHVRWQNSPFG
                     YLDSIKMFAARGEADHATRVQAFMGRAIVQFTRRGVLSGVLPPGENDSPYNTRMIGGT
                     EIAGRRMHAQFLANWQPNLGHAIVSASQDGTIAIARMQEQLGAAIVQATTFRATYEGL
                     RSANQEQLGSATMVALRTGSFARQSYRGELRQPAAVAVTAPHTLPEIPTTTLVVASLM
                     LMGLFMAGLLVAPTLPRVTVGGF"
     gene            5817..5984
                     /locus_tag="VLD60_00905"
     CDS             5817..5984
                     /locus_tag="VLD60_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43535.1"
                     /translation="MVMMIGFVTIGALVWVLVSSMAVESEAEKRRVIVGSEHAVPLPD
                     GAAREQTREAA"
     gene            6362..6652
                     /locus_tag="VLD60_00910"
     CDS             6362..6652
                     /locus_tag="VLD60_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43536.1"
                     /translation="MANMKTITVQVLDVLQRNQTCEFDLLVRDCSEFTWSQLFHEVDR
                     LRRLGQVCLYPVGEGHYFLRLPQKEDSPQENATESQTEIVALQSYKADKPER"
     gene            complement(6818..7114)
                     /locus_tag="VLD60_00915"
     CDS             complement(6818..7114)
                     /locus_tag="VLD60_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43537.1"
                     /translation="MSRINLDPLFTFPDGSHLLISTQCSRDGDFSCALYTAVIAADDG
                     AAFQMISNHLEASTCLSAQEHAYNYAVRHYPRAAELMKKPPYLIWHGPRSLMPQ"
     gene            complement(7145..7408)
                     /locus_tag="VLD60_00920"
     CDS             complement(7145..7408)
                     /locus_tag="VLD60_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB43538.1"
                     /translation="MGKRVLYVGGLSDTISDSQLRDLFGTYGLVARAHVVRHKHSGRS
                     AGYGFVEMGSGEQALEAVVALEGTEFDGNCLRLYVTPYASSGS"
     gene            complement(7710..8003)
                     /locus_tag="VLD60_00925"
     CDS             complement(7710..8003)
                     /locus_tag="VLD60_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43539.1"
                     /translation="MQNQLSLYRMFLAGGMILFAVSFTSPAHAADKAPAPSAKPVATT
                     PGNTLAAGATEDSLQACLSRIPKDATVGQRMIAEQSCRRDEGDRKPFQATGGR"
     gene            8535..9797
                     /locus_tag="VLD60_00930"
     CDS             8535..9797
                     /locus_tag="VLD60_00930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB43540.1"
                     /translation="MDTSVHVNFHALGLSEPLLRDLSHAGLSTPTPIQAQAIPPALAG
                     RDVIGCAQTGTGKTAAFVIPIIERLALLPKGEPQALILAPTRELAIQILTTIDKLGRS
                     LGISATVIVGGADMQAQVRGLRQRPDILVATPGRLLDHMWNGTVNLAPIKILVLDEAD
                     RMLDMGFAPQINQIFDALPEERQTLLFSATLPADLTRMVQASVRNPVQVMVTPSATTA
                     DGVTQAIHYTTHHDKGDLLLSLLREERETVLVFTRTKHRADQVTGVLGRAGHKVAVLH
                     GDKTLPQRRAALEGFKRGTFRVLVATDIAARGIDVANIGHVVNYDLPICPEDYVHRIG
                     RTARMKATGRATSFVTGEEPQQLRAIEQLLGHPVPLAPGSKRPMSGLQPVAHRPHRSP
                     RPSHGRPSRFAGHRHGGEMPLQGSEPAH"
     gene            9897..10190
                     /locus_tag="VLD60_00935"
     CDS             9897..10190
                     /locus_tag="VLD60_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43541.1"
                     /translation="MRIPDGTKVRLREGGQEGVVDGLTELVVGPGRNPDGKTQYRINV
                     GDQDRMLTVETALLVLIDGEGLVMMLKQKPDYRRLVTDRLRGTLPADCFVSVS"
     gene            complement(10471..10752)
                     /locus_tag="VLD60_00940"
     CDS             complement(10471..10752)
                     /locus_tag="VLD60_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43542.1"
                     /translation="MKISGGWRGGKASKKKVRPSYKPKIRWELLGLTDPSMDETATPI
                     ETIRREVSSLASRGFGQGTRSRLTGTDSKSSGRTHLPGQGRTRRRGATD"
     gene            11082..11333
                     /locus_tag="VLD60_00945"
     CDS             11082..11333
                     /locus_tag="VLD60_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43543.1"
                     /translation="MIIQGTSTWFILMLLLSLLSACQREAPAEKASSQPPSQAGSVEG
                     VVRQTIDGIKEPMDKARTVEGTLEKAAERTAEQVQDATQ"
     gene            11919..12281
                     /locus_tag="VLD60_00950"
     CDS             11919..12281
                     /locus_tag="VLD60_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43544.1"
                     /translation="MTIKIEKPTVIQAAGNQPKRIEEFVGLVNSGTAQVSIARMQSPS
                     GWCEPGQTPEFDEYTLVLNGMLKVETKTSVFEVHAGEAIITSRGEWIRYSSPGPQGAE
                     YIAVCLPAFSPLTVHRDP"
     gene            12458..12808
                     /locus_tag="VLD60_00955"
     CDS             12458..12808
                     /locus_tag="VLD60_00955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB43545.1"
                     /translation="MNRFRRHPRIRIAAPFVCSLSPVRPRRWLRRQTIDLGVVYDLSE
                     RGARVSTEASIRPGDEVRLSLRLPKQIRSAEIAVATVRWKKDQFFGLAFRRLSSDAHG
                     RLKKFVAIAAKTSG"
     gene            12815..13192
                     /locus_tag="VLD60_00960"
     CDS             12815..13192
                     /locus_tag="VLD60_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB43546.1"
                     /translation="MVHAQSQVGMTSELRRYPRLRAAPPFAVAFSRLGLQRWQSTDRR
                     GLGVVLDLSIKGVKVMSASAMNPGDHLTMSLRLPDLPASVNVDATVRWGKDHTFGLEF
                     VAVPQSAETRLKKFLSRTAGFHF"
     gene            complement(13335..15659)
                     /locus_tag="VLD60_00965"
     CDS             complement(13335..15659)
                     /locus_tag="VLD60_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSB43547.1"
                     /translation="MQAKSPSQNLQELQQKVEFAEHVKRITNQIHAANNLDQILLDLH
                     KDILSLFDAEDLTLFAFDSDKKEIFSKVPHVDSIEEVRIPITEQSLAGFCAKYLRPVN
                     IADAYNLAELQGIHPSLNHDTSYDKRTGFKTKQVLTYPIVADNKYLMGVLQLLNKKSG
                     SRFTRKDEESVAEIAKALGIALFNLKKVTTKKAATKFDLLVTNGKISQNELDAAIAEA
                     RKSISSDLETILIEKYKVPKIEIGKSIAQFHKCPYIEYSERTLVDIELLRNLNVDYLK
                     KNHWMPLKRDRTAIEILTDDPGDLDRVADIKRTFPGLNIRMAVSLRRDIAQFLNSATG
                     GSDSGGRKLDENVSDILGELVTEAGAEAMEESGLGGGLDENDSAIVRLANQIIADAYR
                     QNASDIHVEPYGEKRETLVRFRVDGDCFEYMKIPQSYRRAIVSRLKIMASLDIAERRK
                     PQDGKIKFKLSESKEIELRVATIPTAGYNEDVVMRILAASEPLPLDKMGFSDRNLKAI
                     REIAEKPYGIILCVGPTGSGKTTTLHSVLGFINTPDIKIWTAEDPVEITQYGLRQVQV
                     QPKIDFTFAKAMRAFLRADPDVIMVGEMRDKETADIGIEASLTGHLVMTTLHTNSAVE
                     TVTRLLDMGCDSFSFADAMLGVLAQRLARRVCKDCKEQYVGTKEEYEEIRQGYGPEHW
                     DKLGIPQDNTFRLARGKGCEICNRTGFKGRVALHELLLGTDKLKRLIQTKAKTEEMVK
                     AAIEDGMTTLMQDGIQKCLQGHTTFKEVKAVAIK"
     gene            complement(15888..17603)
                     /locus_tag="VLD60_00970"
     CDS             complement(15888..17603)
                     /locus_tag="VLD60_00970"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSB43548.1"
                     /translation="MRTSQLLLPTLREDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRTIRKVETIIRQEMNRAGAQELLMPIASPAELWKETGRWDYYGKELIRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQLPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDADETDAKASYQKMYDAYTRIFTRCGLTFRAVEADTGLIGGEVSHEFMVLADTGE
                     ATVVYSNQGTYAANVERAEVLPPTEADTSPQRPLTAISTPQRRTVDEVTRFLKIAPRQ
                     LVKTLLYKAEKETIAVLIRGDHDVNEVKLARLLRVTEVELASPEVVKTLTASPLGFAG
                     PVGLRNVRILADHAVKSMRNFVVGANQADTHYVDANWNRDFTIDQFADLRNAQSGDPS
                     PRGEGTLTLAKGIEVGQVFLLGTKYSKSMNATILDDQGKERLAIMGCYGIGVGRTAAA
                     AIEQNHDDKGIIWPFPIAPFHVHLLPLGQSSAVADATTNLYATLQQAGLEVLWDDRDE
                     RAGVKFNDADLIGAPFQVIVGDKGLAEGLLEIKARKTGEKIKVGPADVPMKILQLASL
                     MHSSR"
     gene            complement(17610..18959)
                     /gene="rseP"
                     /locus_tag="VLD60_00975"
     CDS             complement(17610..18959)
                     /gene="rseP"
                     /locus_tag="VLD60_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HSB43549.1"
                     /translation="MQKAWWFLVVLGILVAFHELGHFLAARWVGVRVLKFSLGFGPKL
                     FGRQMGETEYLLSAIPLGGYVKLFGEDETEAVTPDDRRRSFAHQGLWGKVLIVAAGPG
                     FNFILAYLIFAAWLSTGYTLPVPSFKDIAPVVEAIAADSPAAKAGLMVGDQIKKINGR
                     DIATRVELLDAAAKGKGAPLNLEILRDGRTETLTITPIPTPGQTSTSEEAGYYLGIEE
                     VPPIVNEVVPGKPAAAAGFKEGDRVLSIEGTPIHTWTQMTGLVKENPGKPLHVEVLRE
                     NHRVSLTVTPARETSTVNGQTVEVGKIGIAGPDRSLMKSANPLAAAYNGLEATWKWTE
                     LTVIGLYKMIIGDISSKNIGGPLTIAKISGEAAAQGPSNVIFLIALLSINLGVLNLLP
                     IPILDGGHLLFFLIEGILRKPLGERQREVAQQVGLVLLVGVMIFAFWNDLERIFFSH"
     gene            complement(19085..20245)
                     /locus_tag="VLD60_00980"
     CDS             complement(19085..20245)
                     /locus_tag="VLD60_00980"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSB43550.1"
                     /translation="MKTIVILGSTGSIGTNTLDIVDRFPAEFRVAGLTAGSNDELLET
                     QIRRFKPAMVALSDPAAAARLRQRCLGLSVEILAGQEGLVQVASASQAELVVSAIVGG
                     AGLLPTLAAIRSKKHIALANKEPMVMAGKLMQEEARRHGVRIFPVDSEHSAIFQSLEG
                     HRIKDVRRLILTASGGALWTLAKEELQDVTPERALQHPNWKMGAKITIDSATLMNKGL
                     EVVEARWLFDIPESRIDVMIHRESIIHSLVEYEDRSMIAQLGLPDMRTPISYAMRYPD
                     RLPLDLPSLDLTEIGKLTFCKPDHDRFPCLGLGYESLRIGGTMPAAMNAANEVAVDAF
                     LQGGIRFTDISDIIRSTMDAHAPREVESIDVALEADRWAREKAGSLVHALPR"
     gene            complement(20256..21131)
                     /locus_tag="VLD60_00985"
     CDS             complement(20256..21131)
                     /locus_tag="VLD60_00985"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSB43551.1"
                     /translation="MLECGTTNPEPVQTVRKTATRFDPRRIYTVLVLAPLLYAAIRFF
                     PAPAITFIIALAGGLSLYELYRMCLPQPYHPTTIGIGLFGCTALIVSPHQSSIALPSL
                     LAALLAILSVPLFVHMPLQERLKEGAIALTGMLYIGLTLSFLTLTRLLPDGEWLILFV
                     LLVTWAGDTGAYYVGTLCGQHPLAPRVSPKKTVEGLVGGLIAATVVAYLARWWFLPAL
                     SGLDCLILAMVITATGLWGDLAESAIKRSVGAKDSGGVLPGHGGMLDRLDSLLFSAPA
                     FYYYVTTISQFGSHA"
     gene            complement(21152..21937)
                     /locus_tag="VLD60_00990"
     CDS             complement(21152..21937)
                     /locus_tag="VLD60_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HSB43552.1"
                     /translation="METRPSSKLDQLSDTELTTKLDPDLLPKHLAIIMDGNGRWAEAR
                     SLPRVAGHREGIKSVREMITLCLELGIQALTIYAFSQENWNRPTTEINALMALLEHYL
                     STERNKLIEQGVRFRTIGRTESLPTSALQWVRAAEQETAHLTKLQLTVALSYGGRSEI
                     VDAIRGLVREAQDGKLQPEDIDESLVQQFLYTHDLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRELLLALLEFQRRERRFGRVFSSLSS"
     gene            22147..23580
                     /gene="tldD"
                     /locus_tag="VLD60_00995"
     CDS             22147..23580
                     /gene="tldD"
                     /locus_tag="VLD60_00995"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HSB43553.1"
                     /translation="MDQLIPLDSFGVTEREVLAALGRVKVRDVDYADLYFESCISDSV
                     SMEESLVKRATKSISQGVGVRATAGEKTGFAYSDELTKKDLELAADTARYIANSPAGS
                     QAVPVPVRHRPTRDLYPVKRAEIEVATADRVILLNQIDIEARRYDSRIKNVMASFNTE
                     YKIVMVATSDGTLIGDVQPLSRLQVTCIAEENGNRQIGSFGGGGRIGFEFYRERDRYL
                     DYAREAARIAVLNLSAVEAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSAFS
                     SLLGKRVASGVCTIVDDGTLPGRRGSLNMDDEGTPTSRTMLIEKGILRGYITDKLNAR
                     LMGIPVTGNGRRESYQSIVLPRMTNTFMLAGDSNPDDIIKSVDKGLYAVSFGGGQVDI
                     TNGKFVFSASEAYLIEDGKITKPVKGATLIGSGPEILTKVSMVGHDLKLDEGIGTCGK
                     EGQSVPVGVGLPTIRIDEITVGGTQQG"
     gene            23594..24961
                     /locus_tag="VLD60_01000"
     CDS             23594..24961
                     /locus_tag="VLD60_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSB43554.1"
                     /translation="MSGALQQTEGYIQLASDLLDKAKRSGATEADVIIADGENLAVQV
                     RLGAVDRLSKAREKRLGLRVFVGKRSACTSTSDFSVDSLNQLVAQTCTLAKAVVEDAV
                     SGLPAADQMATEKPDLDLYDETKLDTDRQIDLAKRAEAAAMSTDERITNSEGADFESS
                     SGRVVLGNSHGFIGEYRSSSFSLSVSPIATDPQTGAMQRDAWYAVQRKFDKLDAPEAV
                     GIEAARRTVRRLGARKVGTKRVPVVFDQEMAGSLLGNLCGALSGYSLYKGASFLAGQL
                     GKPLAPDYITVYDDGRMVGGLGSRPFDGEGLATRRNIIVEKGVLKSYLLDTYSGRKLG
                     LPSTGNASRSIGESPSVGPTNFYLVPREQSAEDIIRTVKDGLYVTELIGFGINMVTGD
                     YSRGAGGFWIEDGELAYPVEEITIAGNLKQMFKDIEIVGSDLVFRGRIACPTLKIAEM
                     MIAGN"
     gene            25114..25578
                     /locus_tag="VLD60_01005"
     CDS             25114..25578
                     /locus_tag="VLD60_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HSB43555.1"
                     /translation="MRIVSTAFSNQSAIPALYTCEGKDVSPPMAWSDLPVGTKSLALI
                     VDDPDAPDPAAPKMTWVHWVLYNLPPTSDGLPESVKTLPAGTKEGLNDWKRTGYGGPC
                     PPIGRHRYFHKLYALDTVLPDLKQPTKAKLEEAMKGHIIREAQLVGTYQKDR"
     gene            25750..26265
                     /locus_tag="VLD60_01010"
     CDS             25750..26265
                     /locus_tag="VLD60_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HSB43556.1"
                     /translation="MAPGVSTATDFRLISPTIKDKGTIGNEHVYNGFGCSGSNASPEL
                     RWERAPKGTKSFAVTVYDPDAPTGSGWWHWVIFNIPANTTSLPAGAGKLDGGGAPSGS
                     IQSMTDFGQPGYGGPCPPPGDKPHRYVFTVFALKVDQLPLNKEASGAMAGFYLNQNVL
                     GKASFTAKYGR"
     gene            26585..27040
                     /locus_tag="VLD60_01015"
     CDS             26585..27040
                     /locus_tag="VLD60_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43557.1"
                     /translation="MPLDAEFGKKMLQLITSRYDDRHWRKKIEKTLSLPQSGVSDPIQ
                     QQIFMYLKVGLKAYKSRRADPDSWIIGGYATREVIDRAKFQPKEVGSEIVKDDVAFLG
                     SDPGSEVDEAWWEEMLVQWFDVPEEEKPAEEETVADSAASAEAEASQKK"
     gene            complement(27078..27722)
                     /locus_tag="VLD60_01020"
     CDS             complement(27078..27722)
                     /locus_tag="VLD60_01020"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSB43558.1"
                     /translation="MTLQELAKSLHNCQQCKLAKLGRTQVVFGVGNPHASIMFVGEAP
                     GFNEDQKGEPFVGAAGKLLNDLLQSANLSRDQIYIANVIKCRPPNNRDPEPDEVETCK
                     PFLLQQIQMIRPKLVCTLGNWATQTLLERKVGITKVKAQAFYMKDFVIFPLLHPAAAL
                     HQGGLLDTLKEDFRKLKEFLDRNTKPAELTDASPATIPTLQIEPPDPAQMDLFE"
     gene            complement(27861..29192)
                     /locus_tag="VLD60_01025"
     CDS             complement(27861..29192)
                     /locus_tag="VLD60_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB43559.1"
                     /translation="MASLSILVTLSTPRSVIVIGPGPAGLTAAYRLTLQGHRVTVVSQ
                     SSALGEHLRREGDPPLAILGCHHATWSLLRSLGITPMPTTFHNASLEFLLPNGRLARY
                     PTSQLPAPLHQLITIGQFAALSWGERWKLLSWLEQLWEGALQLAADLEHRTAQDWFES
                     LGHSQSTIQTVWHPLAQWLTGNDLRNLSADAFVAAAKPIFLSRGADNRICVPRQPWHH
                     MFVQPVADRLQQAGATLLLDTRAMQFQFEHERITGVRVQDGRILQADWYIAAVAHHQL
                     TPLLPERWLTRYAYFQQIAELTTTPFTVIQVRTAQTVHASRHIMMGTGPFPWMVCKPS
                     EADKSLVAVLTIPPDQSTIDTQEQVSALLRSLALLQPDSQLTSFRQHEVAHAVLGLQP
                     GTKARRPIQLSPIPNLLLAGAWTDTGWPAILESAIISGERCAEIINGRKPV"
     gene            complement(29168..30034)
                     /gene="hpnD"
                     /locus_tag="VLD60_01030"
     CDS             complement(29168..30034)
                     /gene="hpnD"
                     /locus_tag="VLD60_01030"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HSB43560.1"
                     /translation="MTMTATEAQAFCVNLTKKSGSNFYYSFLFLPKTRRDAMYTIYAF
                     CKAVDSAVDEPPPDSNPKEELRRWRAELDAVYAGTPTWPITISLAHHARELSIPKAYF
                     EELIKGVEMDLLTTRYKTFDELSVYCYRVASIVGLICLHVFGPTSPHAQDYAVDLGMA
                     FQLTNILRDLGADAEQGRIYLPQEDLARFGCTESDLLQRKDSPQLQDLLRFEADRARR
                     FYTKAQVALESLPGSDQRALTVAEIMRALYSRILKRIELPGHQIFGPRARLSTSHRLV
                     VAAGVWLRSRFS"
     gene            complement(30031..30972)
                     /locus_tag="VLD60_01035"
     CDS             complement(30031..30972)
                     /locus_tag="VLD60_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSB43561.1"
                     /translation="MRICSFIPGATEVVAALGLADHLVGISHECDFPSSVRHIPIMVE
                     PVVGHEPLGSADIDRQVKDLASTGQHLYRLNESALLAAQPDVILAQDLCHVCAVTPDQ
                     LRRVLRSFPAAPRMVTLNPASLNDVVNDVERIAAALGQPSKGRDLAQSLRQRIATVRA
                     RSAALPARPRVICFEWLSPLYIGGHWIPEMVEIAGGVDVLGQMNQPSREVGWNEVAVA
                     HPDIVILMPCGFSVERTLAEIASISKFSKDWPRALASWPRTYVVDAGSYFSRPGPRLV
                     EGLELLAALFSGSARTQFDAFVVQDITGTPLAESPSP"
     gene            complement(30980..31675)
                     /locus_tag="VLD60_01040"
     CDS             complement(30980..31675)
                     /locus_tag="VLD60_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSB43562.1"
                     /translation="MQTLSALIFDFDGVIADTEPLHFAGLRQTLSEIGIDLTESQYYA
                     DYLGYDDRGCFIAALTANQRPIEPNTLAQLMTRKAAAYLESVKHHRVIFPGVHEFVRE
                     AASAYPLAIASGALRHEIEYILEEAGLRKEFFSIVSAEDVTKGKPDPQPFLHALKALQ
                     QRTPSLSASSCLVIEDSLPGIRSAKAAGMKVLAVTNTHTVQDLHEAHAITHSLKEASL
                     ADLRTRLWPPTKE"
     gene            complement(31801..32535)
                     /locus_tag="VLD60_01045"
     CDS             complement(31801..32535)
                     /locus_tag="VLD60_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43563.1"
                     /translation="MLMAALAGNLTAVGNPIQSSLFFARQPEPDLEFTEEELDQTTST
                     RSTSPLKPRKKSGGGRPLLWILLLALIAGIGYIAMEPEQLAEWLAPLIGENTPTEPPA
                     VAMRPRPTTPAPPAPSTQPAPVDAVPAPPGPSAAAPAPEIPAPTPSASTPAPTPAAPA
                     SSPVSSPMFGEGQKVTVIGNPTIPAEMIPLTLDPAGTKPGPAVRPGVTLSVLDGDLQP
                     GGWVYSVRADDGTKGWISENRLRLKF"
     gene            complement(32649..34415)
                     /locus_tag="VLD60_01050"
     CDS             complement(32649..34415)
                     /locus_tag="VLD60_01050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HSB43564.1"
                     /translation="MTFSPRLTPLPASPVPRLSPQAKIVLRRRYLARDSRGVVTETAP
                     QLLWRIATDIAQAEQGYASRARLPQAARRFYESMVRLEFLPNSPTLMNAGRPLQQLAA
                     CFVLPVEDSLSSILDAVKQQALIHQSGGGTGFSFSHLRPQRDLVASTSGIASGPISFM
                     RIFNLTTDVIKQGGARRGANMGILRVDHPDILEFIALKQDPTEMTNFNLSVGLTAEFM
                     RALHRRQHYPLVNPRTGKPAKRLSSQLVFDRLVDAAWATGEPGVIFLDTINAANPTPQ
                     LGDIEATNPCGEQPLLPHESCTLGSIDVSKFILTSPTQPRIDYPKLAGTIELAVRFLD
                     NVIDRNHYPLAQIETATRRTRKIGLGIMGFADLLIALKIPYDSEAAIETAGELMAFVQ
                     THAHEASHQLAVQRGLFPAYLGSQLQRQHRPRRNATVTTIAPTGTISIIAGCSPGIEP
                     LYGIKVVRRVMEGLNLTTLHPAFARMVTARGLDLSSLQRALQASPSVQHLTRIPEDVR
                     RLFVTAHDVSPAHHVRMQAIFQQYSDSGVSKTINLPSTATRADVANAFRLAYQLRCKG
                     LTVFRDGSRERQVLSCSQAKPC"
     gene            34525..34938
                     /locus_tag="VLD60_01055"
     CDS             34525..34938
                     /locus_tag="VLD60_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSB43565.1"
                     /translation="MRSYREELWFETTTRRAYINITPHVEAVVKKSGVNEGLVLVNAM
                     HITASVYINDDEQGLLQDYDRFLEAIAPHKAAYRHNETGEDNGDAHIKRQLMGREVVV
                     AITDGRLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
     gene            35064..36077
                     /locus_tag="VLD60_01060"
     CDS             35064..36077
                     /locus_tag="VLD60_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43566.1"
                     /translation="MQSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQSFLEQGRE
                     HDLLTVLAALDEDMAEWLFDLVAEATCSILLDGPVDDKLSYAVMAALELPLQANLTHP
                     LKLKIDPVATADILHRHLRLSAGTVVRVEPRLLTDATLEALTLQALNDHLGTVADSQR
                     ARSIAARLYPPVENTAFLFFELIGVPDSGLPDSLEDPHRRALLEGLVEQCPELALAPD
                     TLEAPVYAAYAMFDAQNAVRLHRLADETAAVWRDLDPLVRARTIVHLHTQCGNGVYMP
                     VWTDLFDPELPDEHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            36216..>36490
                     /locus_tag="VLD60_01065"
     CDS             36216..>36490
                     /locus_tag="VLD60_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43567.1"
                     /translation="MGQPHSSGRIVAGVVTVVGLGIVWLAILMSLPSQQELSAKEPKL
                     SQQPISASKAQLPSEPTLDLSIPGVPSAPPAPSDQDPAATVGGSLSPP"
BASE COUNT         7913 a         9413 c        11191 g         7973 t
ORIGIN      
        1 gattcgcggc aggttgacct atacgtctgc agaaaaggac tccatcaagc tctggacgcc
       61 tgaaggcgaa aaggagtttg cggtcaaacg gaatcgcccc aagtttacga tgtttcaaga
      121 cggagcgctg attaccgtgc agttgaacga caagggtgag gtcatcgatg tgcaccgcca
      181 gttggaattg gagcttgcgg tagcccctgc tcctcatatt gagcctggtt tccggatcaa
      241 gctggaggga gtggtggcag acatcaagtc cgggcaggtg ttcgtgcaga cgccaggggg
      301 acagtacagt ttgaatgcca agacggcccc gcaggacgtc aaggtcggcg acaaactgac
      361 gctctgggtg agtgggaaca atgtcgcgat cgaccaccat gcgaaaggga aagagggtgt
      421 ccatcgcctc attaccggtt cactgaccta tgcctccggg gatatgaaag agatcaagct
      481 catgacgccg gaaggggaac ggacatttgt agttcagcac ggagagagca agctctccgg
      541 gatgaaagag gggacgccga ttaccgtcga actgaacgag gccggcagcg tggtcgaaat
      601 tcgcaaggcc ggatgaacga gtgcagggaa gagaatccgc ggcatggtcg aacggatcat
      661 gccgcgggac tattcacttc cgccgagcga tgggcctgtt tcagacgatc acggtacgtc
      721 gtctgcaaag ccgacgggcg gcagccagac gctcaaggtt ggtcccgccg ccacgcgcat
      781 agattgtggc attaggggac aggacttcga tgttaccctg gcccataact cctcagcttg
      841 tttagactgt cctgtacagg agggtacgga tttcccgaat catcttgtgg ttccaatacg
      901 tttgagcgtg ccgcacatct gttgcagagt ggcattccac atgcagattc acgattgggt
      961 aggaccatga ggacttacaa agcgagcctg atccaggaag gacgtattgg gggcttccct
     1021 ctccggccca aattatcaga catctctcac gcgatgaagt gaaggagtcg ccatgtccac
     1081 atcacatctt ccccgggata agaaagatcg atcggttcga gcgcggaagc acgaccagta
     1141 caaggcgaag ggcaaactcc aggagccgtc ggtgtgtcgt gagtgcaaag cgctctacca
     1201 caaaggccgg tggatgtggg gccctgtccc gtcgggcagt cacgacgtcg tgtgccctgc
     1261 ctgtgaacgg atacgggacg gatcgccgag cggcgtgctg ctgctcaccg gtgagtttgt
     1321 agccaagcat cgggacgaag ttctcgggct cgcccgaaat gaagagaccc gcgtcaaatc
     1381 cgaacatcct ctggctcgca ttatcaagat cgagaaccaa accgaagcgc ccgaaggggt
     1441 ggtcatcacc acgaccgacc cccacctcgc gcggcgtatc ggcgaggcgc tgcatcacgc
     1501 gcatcacggc acattcacct gtcgctatga ggagaacgaa gacctgctcc gggcgaactg
     1561 gcagagctaa gcggagcaac aagggcgagg aatctctgcc aattgattgc aggagatagg
     1621 catggccata tcgtcttggc gtataaaagc cacgcagaag tggggagaac acgctcgatg
     1681 gctcagcggt gagggcccgt ttgctgtgat cgctccctgt cgggagtttg ccttctccct
     1741 ctgggacacg agggaagcag cggacaaagc gaaggcccat ctcgatcaaa tgggatgtgg
     1801 tggaggctgt aaccccctgc ttcataaagt cgttgatctg agtgaatgac ttcgggcgat
     1861 cgagcgagca cggactgggt gacgttgttg tccaccgggc atgggggcag attctctttg
     1921 agtccttctg aaccacgcat aagtctcttc atgccccttc agggaggctg atcacatgtt
     1981 catgtaccta ctcgtgctgc caatcgtcat gggatttccc ctgtcaggct tcgctcaatt
     2041 cgtggagagg ggcgagtata tcgtggtggc caaaggcacg aagctctgtg cgaacatgat
     2101 cctagagcaa tccaaagggg tgctcgatcc aggaaaggtc ttttggcgag tcaccagcaa
     2161 cgtgcgggcc aatggcacgc agaccatcgc ggcatttgcc cgtgaaaccg tgtatgaccg
     2221 caagagcggg gagacgacga tcaaggagag ccgttatgac gaggactatg cgacgttttt
     2281 caaacgagac cctgagcaca aggggatgct cctgatggtc agcccgcaag atggcagagt
     2341 ccaagcgatt gtggaggtct gccagaaggg gagataggtg aggcgccgag tgtgcggtag
     2401 tcatcctctc gtgattgctg aatgacctcg atgaggagca cgtgaagggg aaacctcttg
     2461 ggaagttaac taaagtaggt taatataaga gctaaagaga tgtttttaga agatattact
     2521 tgactattta tggtaaagtg gtccagagta aatcatgaga tgagatcgct cgagaggggt
     2581 tggatgtaga gtcactcgct ctcgcgcatg atgggccgaa aggaacgcgg tgttgacgtg
     2641 tcgtttcaaa ccctgacgtt ccagttacgc tgactgtcct atccgttgcg cttcccgctt
     2701 ccttcctagc gcgatcgatt ccccagtctc cgccgcttcg acgccagagt acctgcaaca
     2761 tgcgacggcc tcgcgagttg tcctcgtaga cgtgtcacga gggtgtcagg ctgtcgccgt
     2821 ggtgacagag tcgacttccc ggttacgtat cgggatcgac ggctgacaag gagatggtgg
     2881 tgtattcaat acggaggttc ttgccatgag actcttaacg attgggatga caggactcgt
     2941 gtttgtcgga atgactacgc tcaccatggc gaatccgtcg atgctgccaa agcatccggg
     3001 atatccgtca ggtggagatt atgccaacga taaaggccaa caaaacctga gctataacca
     3061 atctatggtg gaggccgcga agtccggcga taccaacatg gctccgacgc tcatagatcc
     3121 caacaatgcc aggctggtgg agcatcaggg tgctggtcaa ttgcccatcg tccaagggcc
     3181 gaacattaag atcgagccac cggtgaaaga aggcacgaga atgcccgtga agtaacgcat
     3241 aaggcagagg cttggtgggg ccgttgtcca aggccccgcc gggtgagact gtcgagatga
     3301 aaaagaagca tatttctcgt gctgcgcctg gtgctggtcg gcagtcatac ggacaatgtg
     3361 gcgatccgcg gcgccttgct gaagaagttt ccgaacagcc agggccctgt gatggtttgg
     3421 tcacacctga ggccccgagc atggagaagg ttgatcgtat gattgcagac gttcaggcac
     3481 agatcaagtg gtcgctcagc cacgtcgagt gtgaacgtgg cagcgttcat catggaggga
     3541 gccgtcatga gacacatatc actattcacg acctcgcgag gggcctgggt caggataacc
     3601 ggcttgagca tgctgttcct gttggttggg gctcccgtcg tttcagctga gcccactgcc
     3661 acggaatccg tgaaccgtgt catcacccag gtgatgagca tcctcgatga tcctgcgctt
     3721 aagcagccag cgcggtccgc cgagcgtcgg caggccattg aagacgtggt gaaacagcat
     3781 gtgaattatg aagaaatggc caggcgtgct ctcggggccc cgtggatgga aatcaattcc
     3841 ctcgagcggg aggaattcgt cggtctgttt gtcggcttgt tgcgcgacat gcttgccggc
     3901 cgaatcaacc actattccct caaacaggtc gtctatcttt ccgaataccg tgagggcaac
     3961 gtcgcggaag tgcgtacggc gtggatcgga ggtaaggtcg acacctcgtt cgatgtgcgg
     4021 ttagccaaga aggcaggtga ctggctgatg tatgacgcgg tcatcgacgg ggcgagcatc
     4081 gtcgagaact accgggcaca gttttcccga attatccgtg acgtctcgta tgaggggctc
     4141 gtgagccgga tgaagcagaa aacattgttt gtcaaagcgt ttgaatatgc ggcagttcgc
     4201 tgagttgtcc tgaatgccag aggccgccgt atcgatgtct gcgcggtgtg tgaaggccat
     4261 gacccacaac cgctgaccgt cggatgatga ttgttgcatg cgtgatcgtc gggggccctg
     4321 cgatggcgtt cttacacctg aggccccgat caaggagcgt catatttatg gataagaaaa
     4381 gtatggtgat tggagcggtg gtcatcggaa ccatcgtcgt tgtgggtggg atggtttgga
     4441 gcgctgaaaa agagaagcac aaggaagcgg atcaaacaca ggtaatcgct atggcgcagg
     4501 ccgccaaagt tacgattgat caggcgatca aaacggctct ggataacttt ccagggaagg
     4561 tcatcgaggc ggaattagag aagaaacacg ataaggccgt atgggaagtc gaaattctga
     4621 ccgctgaaca agtcatcaag gtggttcatg tcgatgcgga ttcaggatcg gtgatcgtaa
     4681 ctgaagagaa gccgtcggaa aagaagtaag cgtatgaagg ggaaacggga gcgcggtttc
     4741 atgcgaggag tggagtgcta tggcggggca gacgagtgcc gacatccctg ccacgtgcgt
     4801 cagcggattt cgatttgaag gaggtggcta tgcagcggag gtatgagttt attgatttga
     4861 ttgtcgcgtt gggactcttt gccacgattg tggcaggagg gctgttattc atggctgcca
     4921 acgggacgtt gtccctgtca ctctcccggc agagtgagag agagcagccg atcggcccgt
     4981 cgaatggcac gcagtggttg cagccggttc ttggccaggc gatcctggat cagaacctgc
     5041 ttgatctcca ctatgagaaa gtcacgccgg gtgcggtcac gcaattgaac gaggtgacag
     5101 gcgagcatgt acggtggcaa aactcaccct tcggttatct tgattcgatc aagatgtttg
     5161 cggcgcgggg ggaagctgat catgcgacaa gagtgcaggc attcatggga cgcgcgatcg
     5221 tgcagttcac aaggcgcggc gtcctgagcg gagtattgcc ccccggagag aatgactctc
     5281 cgtacaacac tcggatgatc ggcggaacag aaatcgcggg tcgacggatg cacgcacaat
     5341 tccttgccaa ttggcagcct aatcttgggc acgcaattgt cagtgccagc caagatggga
     5401 cgatcgcaat agcgcggatg caggagcaat tgggtgctgc aattgtacag gcaaccactt
     5461 tccgcgccac ttacgagggc ctacgctccg cgaatcagga gcaactcggc agtgccacca
     5521 tggttgcgct gcgaactggg tcatttgcca gacaatctta tcgaggagaa ctccgtcagc
     5581 cggcggcagt ggcagtgacc gcgccgcaca ccttgccgga gattccaaca accactctgg
     5641 tggtagcatc actgatgctg atggggctct tcatggcggg actgctcgtc gcgcccacat
     5701 taccaagggt tacagtgggg ggattttagg agttcggggg gtgctgcgct ggtgcatccg
     5761 aaagtcgggt agaagaagag actgcatcag tcgtagtctg tatggagagg tggcacatgg
     5821 ttatgatgat cggtttcgtt acaatcggtg cgttggtgtg ggtcctcgtt tccagtatgg
     5881 cagtagagag cgaggcggag aagcggcgtg tgatcgtggg atccgagcac gctgttcccc
     5941 tgcctgacgg cgcggcccga gaacagacta gagaggccgc gtaacgtgag ttgtcgaagt
     6001 gatgttcgtc caagatggtt tgtgaggtct ggactatgga tgggaaagga gcccacagtg
     6061 aatgccgaag agtagcaagg tgtacgtcca cgggttaccc cctacgacaa ccgacgaaga
     6121 attggatgga ttgtgtggac cacatggaag agtgaagtcg gcccgcgtgg tgttgttgtt
     6181 cgataatata accaggcaga ggcaggggtt cgggatcatc gaaatggcat ccgcaggtga
     6241 caatcaaaaa gttgttgggg cgctccatgg aagcaagctt gatgaatcga tcctgcgctg
     6301 ttttgtcttg ccagttcaac ttcatgagat tgcacaagaa tgatacagag gggaaaaatc
     6361 tttggctaac atgaagacca ttacggttca ggtgttggat gtgcttcagc gcaaccagac
     6421 ctgtgaattc gatctccttg tgcgtgactg ttcggaattc acgtggagtc aactgtttca
     6481 tgaagttgac cggttacgcc gcttgggtca ggtctgtttg tatccggtcg gagaaggcca
     6541 ttactttctt cgactcccgc aaaaggaaga cagcccacaa gagaatgcaa ctgaatcaca
     6601 gacggagata gtcgccttac aatcctacaa ggcagacaag ccggaacgtt gatcgttcac
     6661 gccgacgtca ttgcgatgca attagccgcg gacgatctca tcgccgtcta gcctgtagat
     6721 agatatgagg gtgtcgatcc gataggggac ggagctgccg agagaattgc cttagacccg
     6781 cccgagggca gttgcctatt caccacaagt tcaagaatta ttgaggcatg agcgaacgag
     6841 gcccgtgcca gatcaggtac gggggcttct tcatgagctc agccgcacga gggtaatgcc
     6901 gaacggcata gttgtaggca tgctcctggg cgctgagaca ggtggacgct tcaagatggt
     6961 tggagatcat ttgaaaggct gcgccatcat ctgccgcaat gacagcggta tagagggcgc
     7021 aggagaagtc tccgtctctt gaacattggg tgctgatgag cagatgcgac ccatccggaa
     7081 atgtgaatag cgggtctaag ttaatgcggc tcatgggtgc tcctcacgat gtcgacaaaa
     7141 attgttacga accactggac gcgtaggggg tgacatacag ccgcaaacaa ttgccgtcga
     7201 attccgttcc ttcaagggcg accacggctt cgagagcctg ctcgccggat cccatttcca
     7261 cgaatccata accggccgat cttccgctgt gcttatgccg gacgacgtgg gcccgcgcaa
     7321 ccagcccata ggtgccgaag aggtcgcgaa gctggctgtc agagatggtg tccgagaggc
     7381 cgccgacata caagacccgt ttccccatag ctgctgctcc ctattccccc tgtactggtc
     7441 aagcccgtgg cttacgtttg gtcccgtggt tcgaaatagg cataggtcag aacgccgaag
     7501 actcctaggg catacaacat cgtggccccc gcgagaggat agtgggcaaa ccactgaaca
     7561 atttgcgtga gtgcatccat aaaaattcca gaccttttcg tcctattgcc tcgatgaaca
     7621 ggcatcattg gcgtgacatg gtgaaaccaa tgatgaaacc gcagcctggc ttgcgacaga
     7681 ggccaggctg cgacgggaca cgtaaatgtt taccgaccgc cggttgcttg gaagggtttc
     7741 cggtcacctt cgtctcgccg acaactctgc tcggcgatca tgcgttgccc aactgtggca
     7801 tccttgggaa ttctggaaag gcaggcctgg agagaatctt cggtcgctcc tgccgccaag
     7861 gtattgccgg gggttgttgc aactggtttc gccgatggag ccggggcttt gtccgctgca
     7921 tgagcaggtg atgtgaagga gactgcgaag aggatcatgc ctccggcgag gaacatacgg
     7981 tatagagata gttggttctg cataaaatgt ccttcctgtg atgaggtcga agaaagagga
     8041 cactcgtcgt ggccccgatc cgatcgagtg actacgttct ggtggacttg cgcggacaga
     8101 tctcgctagc cggacgttcc gaggaacggc ggaaactatg gaatcgatcg cgcaggcacc
     8161 ggagtagagg agggaggcgc gagggatagg atagtgaccg tggttcgtaa cgttgctaga
     8221 gtatcacagt tcggcggtaa tagcgaatac accgatggca aggcaggcac gggaagaaag
     8281 ggcgcttaaa tgaaccggaa aatgagggga aaatgaaggg aaaatcaagg aagaggttga
     8341 cggcggggaa ggcatgctgt attgtgtgcg tacatgtcct ctcattgtag tccggtgagg
     8401 ggatgtgcaa atcacaacgg acttcgcgat cgcatcgcga ccaggtccag ttcggagttg
     8461 cacccttccc ggttgtctct cccctcagtt ctctgatcca ggcagtatgc gatcacactg
     8521 aaaggattgc tgtagtggat acgtctgttc acgttaattt tcacgccctt ggtctgtctg
     8581 aacccctctt acgtgatctc agccacgccg gattatcaac accgacaccg atacaggcgc
     8641 aggccattcc gccggctctg gcgggacgag atgtgatcgg ctgtgcgcaa accggtacgg
     8701 gaaagaccgc ggcgtttgtc attccgatca tcgaacgact ggcgctcctg ccgaaaggag
     8761 agccgcaagc gctgattctg gctccaacaa gagagctggc catccagatt ctgacgacga
     8821 tcgataaact cgggcgcagc cttggcatct cggcaacggt catcgtcggg ggggccgata
     8881 tgcaggcgca ggttcgagga cttcggcagc ggccggatat tctcgtggca actccggggc
     8941 gcttgctcga ccatatgtgg aatggcaccg tcaatctggc acccatcaag atcctggtgc
     9001 tcgacgaagc tgatcgcatg ctggacatgg gatttgcgcc gcaaatcaat caaatattcg
     9061 atgccttgcc ggaggagaga caaacgttgc tgttttctgc cacgctgcct gccgatctga
     9121 cgagaatggt ccaggcgagt gtgcggaatc ccgttcaagt catggtgacg ccttcggcga
     9181 cgacagccga tggggtaacc caagcgatcc attatacgac gcatcacgac aagggagatc
     9241 tcttgctgtc gctcctaagg gaagagcggg agaccgtgtt ggtattcacg cgcacaaaac
     9301 atcgggccga tcaggtgacc ggagtgctcg ggcgagcggg gcacaaggtg gctgtgctcc
     9361 atggagataa aaccttgccg cagcggcgag cggcgctgga aggattcaaa cgggggacct
     9421 ttcgtgtgtt ggtcgccacg gacattgcgg cgagggggat cgatgtggct aatattggcc
     9481 atgtcgtcaa ctacgattta cccatctgcc cggaagatta tgtccatcgc atcggacgga
     9541 cggcgaggat gaaggcaacc gggcgggcga cgagtttcgt tacgggcgaa gaaccgcaac
     9601 aacttagggc gatcgaacag ctcttgggac accctgtgcc tctggcgcct ggcagcaagc
     9661 gtcctatgag cggtctgcag ccggtggcgc accgaccgca tcgcagtccg cgaccttcgc
     9721 acggtcggcc atctcggttc gcagggcacc gccatggggg agagatgcct ttgcaaggaa
     9781 gcgagcccgc gcactaggcg gtgtgagtga agcgcatctc ttaattcatg tcgtaagcga
     9841 aaggaaatgg tatgtcacag gattcagtga tagcagtgag taaggcgagc ccgtggatga
     9901 ggatccctga cgggaccaag gtccggctgc gtgaaggtgg gcaagagggc gtggttgacg
     9961 ggttgacgga actggtggtc gggccggggc gcaatcctga cggcaagact caatatcgga
    10021 tcaatgttgg cgatcaggat cgcatgctca cggtcgaaac cgcgctgctc gtcctcattg
    10081 atggagaagg gctggtcatg atgctgaagc agaaaccgga ctatcgccgt ctcgtgacgg
    10141 atcggcttcg cgggacgctc ccggcggatt gctttgtctc agtgtcctaa ccagatcaga
    10201 gtgtaagccg cccctcatcg gggcgcttta gaacagcggg gcttgtggtc cccagcggag
    10261 gccagccaaa tcgggatctt ctccgtcggg ggtggagctt cgcggggctt tctccgcctt
    10321 gcggagtgca cgcttggctt ccttctcctt ccggttcatt tgcagcgatt tctctcggtc
    10381 ccgtttcgcg agcgttgttt ttcctgcgcg tccgatggtg tcctccttgg tgtgaaggtg
    10441 atgaggttat tgccggtcta gtgccgacgc ctaatcggtg gctccacggc gtcttgttct
    10501 gccttgtcct ggcaggtgag ttctgccaga agacttgctg tcggtaccgg tcaatcgtga
    10561 gcgagtaccc tgtccgaatc cacgactggc aagcgaggac acttctcgcc gaatcgtctc
    10621 gataggagtt gcggtttcat ccatgctcgg gtccgtcaat cccagcaact cccaacggat
    10681 tttcggcttg taagatggtc ggaccttctt cttgcttgcc ttgccgcctc tccatccccc
    10741 agagatcttc atgagtcact cctttttcgt gtcccaggct cgagcgggtc gactgtggct
    10801 cgctgagcag ggctcgtctc aatgttggaa ccggactttc ccctctggcc aagcgctccc
    10861 aggcatggca tgcgggccgt aagagcagct gaaacttcga gatcagtcgc gcaagggctg
    10921 gaccggggaa taggaacgcg cgagggattg aaacgtggct cgtacgggaa agcattgtag
    10981 catgctttcg cgaagagggc aaatacggta acgagatggc agagatcgga atatgcggta
    11041 ttattgcgta gtttgctgat ccgggccggt tggaggaatc gatgatcata caaggtacaa
    11101 gcacatggtt tatcttaatg ctgctcctga gcctcctttc tgcctgtcaa cgggaggctc
    11161 cagccgaaaa ggccagctct cagccaccga gccaagctgg ttcggttgag ggagtggttc
    11221 ggcagaccat cgatgggatc aaggagccaa tggataaagc gcggacggtg gaagggacgc
    11281 tcgaaaaggc agccgaacgg acagccgagc aggtgcaaga cgcaacccag tagtggttgc
    11341 agcttcgttc ttcgccgact cagaaagcgg gaaaaacatt aaagctcctt cccatacctt
    11401 gtggaaaaag cctcaagagc cattcggctt cccttccatg tgcccccgga ccgtctgtca
    11461 gtggtattgg aaatagctga ggagaagtct atcctgccgc ctgttgagtt ccgagtgagc
    11521 aggtcttcac cagacgctca cggctcctga tcaaaatgat ccgagcaggg gttatagtct
    11581 tcatagccgc ggcaggctgt acggcttaca cctgtcgtga agtcgacatg tgggatttcg
    11641 tggggtaaga gcctcgattg gagaattgat cgctataagg tcagcgttgg gcatgacgga
    11701 aaaacggaga aatctccata cagtcaaagc accggccact gatgtatcgt ccgaagctgt
    11761 tggcgtacag gggaaaggga ggtggcaggt gaccattcag gcccgtctga aggatggaag
    11821 ctgtagcgag ccagcgctcc catgtcgttt aaggttgtcg atttatggct tgaacaatcg
    11881 ctgggcaggg acatgcgaag aaggagattg gaaacttcat gacgatcaaa attgaaaaac
    11941 ctacggtgat tcaggctgcg ggcaatcaac caaagcgcat cgaggagttt gttgggctgg
    12001 tgaacagcgg cacggcacag gtcagcatcg ccagaatgca gagtccttcc ggatggtgcg
    12061 agccggggca aacgccggaa ttcgacgaat atacgctggt actcaatggg atgctgaagg
    12121 tagaaacgaa aacaagcgtg ttcgaggtgc atgctggtga agcgatcatc acttcgcgcg
    12181 gggagtggat ccgatacagt tctcctggcc ctcaaggagc ggaatacatc gccgtctgcc
    12241 tgcccgcgtt ttctcccctt actgtgcatc gtgatcctta gagccgtgtg tccttccggt
    12301 atggtcaact tcctgcgttg gctgaacgct acgtagcata gctgaatttc cttgctgctc
    12361 gattctcaga aagcattttc tgaacaggcg ttttttgtgt acagtacctc ctatggaagg
    12421 accgactgtt gagtatgagg gccatcgacc ctccttcatg aatcgtttcc gccggcatcc
    12481 gcggatacgg attgcggctc cattcgtctg ttccctttcg ccggttcgac cgagacgctg
    12541 gttgagacgc cagaccatcg atttgggagt tgtctacgat ctgtctgagc ggggcgcgcg
    12601 ggtgagcacg gaagcatcga taaggccggg agacgaggtc aggctgagtc tgcggcttcc
    12661 gaaacagatt cggtcagccg agattgctgt ggcaaccgtg cgatggaaga aggaccagtt
    12721 tttcggtctt gcgtttagac gactctcatc ggacgctcac ggccgcttga aaaagtttgt
    12781 cgccatcgcg gccaaaacct caggataagt catcatggtg catgcccaga gccaagtcgg
    12841 tatgacatca gagttgcgtc gttatcctcg tctgcgcgcg gccccgccgt tcgccgtcgc
    12901 attttcccga ctggggttac aacgctggca gtctaccgat cgaaggggat tgggcgtggt
    12961 gctcgacctt tcgatcaaag gtgtgaaagt gatgagcgcg tcggccatga acccggggga
    13021 tcatctgaca atgagtttgc ggctgccaga cctgccggct tccgtgaatg tggatgccac
    13081 ggttcgttgg ggaaaagatc acacgttcgg gttggagttt gtagccgtcc cgcaatcagc
    13141 tgaaactcgc ctcaagaaat tcctttcgcg tactgcaggg ttccacttct agcggcattc
    13201 ccccattcat tgatggagta gaggttcggg gatttgaatg gcctgtatca ggttccacag
    13261 cggtgtgact tgtggtgtcg ggaaaggacg agatgggtcc cgtccggcaa gtgccggacg
    13321 ggacccgagc agggttactt aatggccacg gcttttactt ctttgaaggt ggtatgacct
    13381 tggagacatt tttggatgcc gtcctgcatg agggtcgtca ttccgtcctc aatcgcggct
    13441 ttgaccatct cttccgtttt cgccttcgtc tgaatcaacc gcttcaattt gtccgttccg
    13501 agcaataatt cgtgcagggc cacgcgcccc ttgaatccgg tccggttgca gatttcacat
    13561 ccctttccac gggcgagacg aaacgtattg tcttgaggga tgccgagttt gtcccagtgt
    13621 tcagggccgt agccttgacg aatctcttcg tattcttctt tcgttcctac atattgttcc
    13681 ttgcagtcct tgcagacccg gcgtgcgaga cgctgggcga ggacgccgag catggcatcc
    13741 gcgaaactga aggagtcgca tcccatatcg agcaaccgcg tgacggtttc gaccgcactg
    13801 ttggtgtgga gcgtggtcat gaccaggtgg ccggtgagcg aagcttcaat gccgatatcc
    13861 gccgtttctt tatcccgcat ttcgccgacc atgatgacgt cggggtcggc acggagaaag
    13921 gctctcattg ccttggcaaa cgtaaaatcg atcttgggct gtacttgaac ctggcggagt
    13981 ccgtactgcg tgatttcgac cggatcctct gccgtccaga tcttgatatc gggtgtgtta
    14041 atgaatccga gtactgagtg cagggtggtg gttttacctg aaccggtcgg tccaacgcag
    14101 aggatgatgc cgtagggctt ttccgcgatc tctctgatgg ccttgagatt ccgatccgag
    14161 aaccccatct tgtcgagcgg caagggttcg ctggccgcga gaatacgcat gaccacgtcc
    14221 tcgttatagc cggcggtcgg gatggtggcg acgcgcaact cgatttcttt cgattcactg
    14281 agtttaaact tgatcttccc gtcctggggt ttgcgacgtt cggcgatgtc gaggctggcc
    14341 atgatcttga ggcgagagac gatcgcacgg cggtagcttt gcgggatctt catgtattcg
    14401 aagcaatcgc cgtcaacccg gaagcgaacc aatgtttcgc gcttttcccc atagggctcg
    14461 acgtgaatat cggaggcatt ttgtctgtag gcgtcggcga tgatttggtt ggcaagacgg
    14521 acgattgcac tatcgttttc atccagtccg cctcccagtc ccgactcttc catcgcctcg
    14581 gcccccgcct cggtgaccaa ttcgccaaga atgtcggaga cgttttcgtc tagcttgcgg
    14641 cctcctgaat cgcttccccc tgtcgctgaa ttaaggaatt gagcgatgtc tcggcggagg
    14701 ctgacggcca tacggatatt gagccccggg aaggtgcgtt tgatgtcggc aacccggtcg
    14761 agatcgcccg gatcgtccgt gaggatttca atcgcggtgc ggtcccgttt caacggcatc
    14821 cagtggttct ttttcaggta atcgacgttc agattcctga ggagttcgat gtcgacgagc
    14881 gtccgttcgc tgtactcaat ataggggcat ttatggaact gtgcgataga tttcccgatc
    14941 tcgatcttcg ggactttgta tttctcaatc aggatggttt cgagatcgga tgaaatgctt
    15001 ttccgggcct cggcaatggc ggcgtccagt tcattctgcg aaattttccc gttggttacg
    15061 agcagatcga actttgtcgc ggctttcttc gtcgtgactt tcttgagatt gaagagcgcg
    15121 atacccaggg ccttggcgat ctctgcaacg gattcttcat ctttcctggt aaaccggctg
    15181 ccgctcttct tgttgagcaa ttggagaacg cccatcaagt acttgttatc ggcgacgatg
    15241 ggataggtca ggacttgctt tgtcttgaag ccggttcgtt tgtcgtagga cgtgtcatgg
    15301 ttcaaggaag gatggatgcc ttggagttcc gcaagattat aggcatcggc gatgttcacc
    15361 ggacgcaggt acttggcaca gaatccggcc aggctttgct cggtgatcgg gatgcggacc
    15421 tcttcgatcg agtcgacgtg ggggaccttg gaaaaaattt ccttcttgtc ggagtcgaag
    15481 gcaaagaggg tgagatcctc ggcgtcgaag aggcttaaga tgtctttatg aagatccaga
    15541 agaatctggt cgagattgtt ggccgcatga atctggttag taatccgttt gacgtgctcc
    15601 gcgaactcga ctttttgctg aagttcctgc aaattctggc tcggggattt tgcctgcatt
    15661 cctgcactcc tctcaataaa accagaggct tgtgtttggt cgatgctgcc tgtggccgaa
    15721 gatgttatct gtccacgacc gctttcacga ggatcgagac tcagtctaga ccaatagggg
    15781 aggaaggcaa ggaaaagata ttttctcgat gaccgcgaac aacgggtggc cgtgaaagac
    15841 aggccaagag gtacggttaa agtgagaaga taataaggga gagcaaatta ccgggaagag
    15901 tgcatcaatg aggcgagttg caggattttc atgggaacgt cggccggccc gaccttgatc
    15961 ttttcccctg ttttccgagc cttgatctcc aggagtcctt ccgcgagacc cttgtcgccc
    16021 acaatcactt gaaacggtgc gccgatcagg tcggcgtcgt tgaacttcac gccagcccgt
    16081 tcgtcacgat cgtcccataa cacttcaagg ccagcctgtt gcaaggtagc gtacaggttc
    16141 gtcgtcgcgt cagcgacggc ggaggattgg cccagcggca agaggtggac atggaacggc
    16201 gcgatgggaa acggccagat gatgcctttg tcgtcgtgat tctgctcgat ggccgcggca
    16261 gcagtccgac ccaccccgat gccgtaacat cccataatgg ccaaacgctc cttaccctga
    16321 tcgtcgagga tcgttgcatt catgctcttg ctgtactttg tgccgagcag aaagacttgt
    16381 cccacttcga tgcccttggc gagcgtcagg gtgccttccc cgcgcggcga gggatcgccg
    16441 ctttgcgcat tacgcaggtc agcaaactga tctatcgtga aatcgcgatt ccaattcgca
    16501 tcaacataat gcgtatcggc ctggttggcc cctaccacaa agtttctcat gcttttgacc
    16561 gcgtgatcgg cgaggattcg tacgttcctg agccccaccg gaccggcaaa gccgagcgga
    16621 gacgccgtga gggttttcac gacctcgggt gaggccagct cgacttctgt cacgcgaagg
    16681 agccgtgcca gcttgacttc attgacatcg tggtcgccac ggatcaggac cgcaattgtt
    16741 tctttctccg ccttgtacag aagcgtcttt acaagttggc gtggagcaat tttcagaaat
    16801 cttgtcactt cgtcgaccgt tcgtcgttgc ggcgtcgaaa tggcggtcaa cggacgttgc
    16861 ggggaggtat cggcttctgt tggcgggaga acttccgccc gttcgacgtt ggcggcatag
    16921 gtgccctggt tgctatacac gaccgtggcc tccccggtgt cggccaagac catgaattca
    16981 tgggatactt cgccgccgat caagcccgtg tctgcttcga cggcccggaa ggtaagtccg
    17041 catcgagtaa agatacgggt gtaggcgtcg tacatctttt ggtagctggc cttcgcgtcc
    17101 gtctcatcgg cgtcgaagct ataggcgtcc ttcataatga actcacgccc gcgcatgagt
    17161 ccgaaacgag gacggatttc atctcgaaac ttcgtctgga tctggtagaa attcagcggg
    17221 agttggcgat aggagcggac ctcccggcga aagagatctg tgatgacttc ttcgtgggtg
    17281 gggccgagac aaaaatcccg ctcatgccga tctttgaagc ggatcaattc ctttccgtag
    17341 tagtcccacc gtccggtttc tttccagagt tctgccggcg aagcgatcgg cataagcagt
    17401 tcttgcgcgc cggcgcggtt catctcttgc cggatgatcg tctcgacctt ccgaatggtg
    17461 cgaagcccga ggggaaggta agtatagatg ccggctgcca ctttgcggat gagtcctgca
    17521 cggagcatca accgatgact gacggtttcg gcttcccctg ggtcttcccg cagggtaggg
    17581 aggaggagtt gagatgtccg catgggatgt cagtgcgaga agaagatccg ttcgaggtcg
    17641 ttccaaaagg cgaagatcat gacgcccacc aataacacca gtccgacttg ctgcgcgacc
    17701 tcgcgctgcc gttcgcccaa cggcttgcga agaatgcctt cgatgaggaa gaaaagcaag
    17761 tgccccccgt cgaggatggg aatgggcaga aggttcagca cgccgagatt gatgctcaag
    17821 agagcgatca gaaagataac attggagggt ccctgggctg cggcctctcc ggagattttt
    17881 gcgatcgtca agggaccgcc gatgttcttg ctcgaaatgt caccgatgat catcttatag
    17941 agaccgatta cggtgagttc ggtccacttc cacgtcgcct caagcccgtt ataggccgca
    18001 gccagcgggt tcgcggactt catcaaagaa cgatccggcc ctgctatgcc gatctttccc
    18061 acttccaccg tctgcccgtt cactgtggaa gtttcccgtg ctggcgtcac ggtcagcgat
    18121 actctgtggt tttccctcag cacctccaca tgaagcggct tccctgggtt ttcctttacc
    18181 aatcccgtca tctgggtcca ggtatggatc ggagttccct cgatggaaag gacgcggtcg
    18241 ccttctttga atcccgcagc ggcggctggt ttcccaggta ccacttcatt gacgatcggc
    18301 gggacttcct cgatacccaa gtagtatccg gcttcttcgc ttgtcgaggt ctggcccggt
    18361 gtcggaatcg gagtgatcgt gagggtttcg gtgcggccgt cccgcaagat ctccaggttc
    18421 aggggcgcgc ccttgccctt tgctgcggca tcaaggagtt cgacccgggt ggcgatgtcc
    18481 cgtccattga tcttcttaat ttgatcgccg accattagac ctgcttttgc cgcgggagaa
    18541 tctgcggcga tggcctcgac gacgggggcg atgtccttaa aagaagggac tggaagagtg
    18601 tatccggtcg acaaccatgc agcaaaaatg agataggcca gaatgaaatt gaatcccggc
    18661 ccggctgcaa cgatgagcac ttttccccac agaccttgat gcgcgaagga ccgtcggcga
    18721 tcgtccggcg tcacggcctc ggtctcatct tctccgaaga gcttcacata gccgccgagc
    18781 gggatggcgg agagtagata ttcggtttca cccatctggc ggccgaagag tttcggaccg
    18841 aatcctagtg agaacttgag tactctcaca ccgacccagc gggcggcgag aaaatggccc
    18901 agttcatgga aagcgacgag aatgcccagc acgacaagga accaccaggc tttttgcatg
    18961 agaagccaca tgctatcagg agaccatgtc aatgcagaca tcatgattgg tgctcagttg
    19021 ttaataatga atggaatgtc agattcaacg taacatgttt cggttaatat tcaaggtaat
    19081 gcggtcagcg cggtaaggca tgaaccaaag atccggcctt ttctctcgcc caacggtctg
    19141 cttcaagcgc cacgtcgatg ctctcgactt cacgcggagc gtgcgcatcc atggtgctac
    19201 gaataatatc agaaatgtct gtgaagcgta tgccgccttg gagaaatgca tccaccgcga
    19261 cttcattggc tgcgttcatg gccgcaggca tggttcctcc gatccggagc gattcgtaac
    19321 cgagtccgag acaagggaac cgatcgtgat cgggtttaca gaacgtcagc ttcccgatct
    19381 ccgtgagatc aagcgaaggc agatccaacg gaagccgatc gggatagcgc atggcataag
    19441 aaatgggggt ccgcatatcc ggcagcccca actgcgcaat catcgagcgg tcttcatact
    19501 ctaccaaaga atggatgatg ctttctcgat gaatcatcac atcgattcgc gattccggaa
    19561 tgtcgaacag ccagcgtgcc tcgacgacct cgagcccttt attcatgagg gtcgcggagt
    19621 cgatcgtaat ctttgcaccc atcttccagt tggggtgctg cagcgcgcgt tcgggggtca
    19681 catcctgaag ttcctccttc gccagggtcc agagcgcccc tcccgaggct gtgaggatga
    19741 gacggcgtac atctttgatg cggtggcctt cgagcgattg gaaaatcgcg ctgtgctcgc
    19801 tatcgacggg aaagattctg acaccgtggc ggcgcgcttc ttcctgcatc aacttgcctg
    19861 ccatgaccat cggctccttg ttggccaggg caatgtgctt cttgctgcgg atggccgcaa
    19921 gcgtcggaag aaggccggcg ccgccgacga tggcggacac aaccagttcg gcttgagatg
    19981 ccgaggccac ctgaacgagt ccttcttggc ctgccaggat ttcgacagag aggccaagac
    20041 agcgctgacg cagtctggcc gcagcagctg ggtcagataa cgccaccatg gcgggtttaa
    20101 atcgacgtat ttgtgtttcg agcaattcgt cattgctacc ggcagtcagt cctgccacac
    20161 gaaattcggc tgggaatcga tcgacgatgt ctagggtgtt ggttccgatc gacccggtcg
    20221 agccgaggat gacgatcgtt ttcatagcaa ctccttcacg cgtgcgatcc gaattgactg
    20281 atcgtggtca catagtagta gaaggcgggg gcactgaaca agagactatc gagacgatcg
    20341 agcatgccgc cgtgcccggg taacacgccg ccggaatctt ttgcaccgac gctccgtttg
    20401 atcgctgact ctgcgagatc gccccacaaa ccggtggcgg taatgaccat tgccaggatc
    20461 aggcagtcga ggccggatag agccggcaag aaccaccagc gcgcaaggta ggcgacgacc
    20521 gttgcagcga tcaatcctcc gaccaagcct tccaccgtct ttttcggact gacgcgcggg
    20581 gctaatggat gctggccgca gagggtgccc acatagtagg caccggtatc gccggcccag
    20641 gtcactaaca gcacaaacag gatgagccac tcgccgtcgg ggagcagtct tgtcagggtc
    20701 agaaaactga gggtcagccc gatatagagc atgccggtca aagcaattgc gccttccttg
    20761 aggcgctcct gcaatggcat gtgcacaaag agcggaaccg ataggattgc cagcagcgca
    20821 gccaagaggc ttggaagggc gatgctggat tgatggggtg acacgatgag cgctgtgcag
    20881 ccgaagagtc cgatgccgat cgttgtcggg tggtatggct gtggaagaca cattcggtac
    20941 aactcataca atgacagccc gccggccaac gcgatgatga aggtgattgc tggggcgggg
    21001 aaaaaccgaa tggcagcata gagcagcggg gccagcacaa ggacggtgta aatacggcgg
    21061 ggatcaaatc gagtcgcagt tttgcggaca gtctggaccg gctccgggtt cgtcgttcca
    21121 cactccagca tggcggtgtt tcctgtgcag actacgacga aaggctgctg aatactcggc
    21181 caaaacgtct ttcccgtcgt tggaactcga gcagagccag caagagttcc cggcgtcgga
    21241 agtcgggcca tagggttggc gtaaagtaga gttcggtgta ggccagttgc cagagcagaa
    21301 agttgctgat gcgcgtttcg ccgctcgtgc gaatcaacag gtccggatcg gggaggtcgt
    21361 gtgtatagag gaattgttgg actaatgatt catcaatatc ttccggctgg agcttgccgt
    21421 cctgggcttc acgaactaac ccgcgaatcg cgtccacgat ctctgaacgc ccgccgtagc
    21481 ttaatgcgac tgttaattga agcttcgtga gatgggcggt ttcctgctcc gctgcgcgaa
    21541 cccattggag cgccgaggtc gggagggatt ccgtgcgtcc gatcgtgcgg aagcggacgc
    21601 cctgttcgat cagtttattt cgttccgtcg aaaggtaatg ctccaagagc gccattaagg
    21661 cgttgatttc cgtcgtaggt ctgttccagt tttcctggga aaacgcgtaa atcgttaacg
    21721 cttggattcc gagttccaga cacagagtaa tcatctcacg gacggacttg attccttcgc
    21781 gatggccagc gactcgggga aggctgcgtg cctccgccca gcgaccattt ccatccatga
    21841 tgatggctag gtgcttggga aggaggtcgg gatcgagttt tgtcgtgagc tccgtgtccg
    21901 aaagctgatc gagctttgag gaaggcctgg tttccatagg gtctggtttt tgtaatgatc
    21961 ctttctctaa agtacgcagg gtaggtgagc aaagtctact gaccgggatg ggcaaagtca
    22021 aacgatattt cgtaattctc ctgaaaaagc gacgagttgg atgtgttcgc attgcgcctg
    22081 ctgaaatgaa tcggctatac tccttcttcc cgcagataaa cctccgactc tgaggcgttc
    22141 aacgatatgg accagcttat tccgcttgat tcgttcggtg tgaccgagcg cgaggtgctc
    22201 gccgcgttag ggcgtgtaaa ggtccgcgac gtcgactatg cggatttgta cttcgagtcc
    22261 tgcatatccg actccgtatc gatggaggag tcgctcgtca aacgagcgac caaaagtatt
    22321 tctcaggggg taggagttcg tgcgacggcg ggagaaaaaa cgggatttgc ctattccgac
    22381 gaattgacga agaaagatct tgaactggcc gccgataccg ctcgctatat cgccaattcg
    22441 ccagctgggt ctcaggcagt ccccgtgccg gttcgccatc gccctacccg tgatctctat
    22501 cctgtcaaac gggcagagat cgaggtcgcg acggctgatc gcgtcatcct gctgaaccag
    22561 attgatatcg aggcccgccg ctacgactcc cgtatcaaga atgtcatggc ctcgttcaac
    22621 accgaataca agattgtgat ggtggcgaca tcggatggca ccctcatcgg cgatgtgcaa
    22681 ccgctctcgc ggctccaagt cacgtgcatt gctgaagaga acggcaatcg acagattggg
    22741 tcgttcgggg gcggcgggcg aatcggattc gagttctatc gtgaacggga ccggtacctg
    22801 gactatgcgc gagaagccgc ccgcattgcg gttcttaatc tgtctgctgt ggaggcgcct
    22861 gctggcgtga tgccggtcgt gttgggaggg ggttggcccg gcattctgct gcatgaagcg
    22921 atcgggcacg gactcgaagc ggatttcaat cgcaagaaaa cttcggcctt ttccagtttg
    22981 ctcggcaaac gcgtggcatc cggtgtctgc acgatagtcg atgacggcac gttgccgggc
    23041 cggcgcggtt cgctcaacat ggacgacgag gggacgccga ccagccggac gatgttgatc
    23101 gagaagggga ttcttcgcgg ctacattacg gacaagttga acgcgcgact gatgggaatt
    23161 ccggtgaccg ggaatggtcg gcgggaaagt taccagagca ttgtgctgcc tcgcatgacg
    23221 aatacgttca tgctcgcagg ggattcaaat ccggacgaca tcatcaagtc cgtcgataaa
    23281 gggttgtatg cggtgtcgtt tggcggtggt caggtcgaca tcaccaatgg gaaattcgtg
    23341 ttctcggcca gcgaggcata cttgattgaa gacggcaaga ttaccaagcc ggtcaaaggg
    23401 gcgacgctga tcggcagtgg cccagagatt cttacgaagg tctcgatggt ggggcatgat
    23461 ctgaaactcg atgaagggat cggcacctgc ggaaaagaag ggcaatccgt gccggtcggc
    23521 gtcggccttc ccacaatcag gatcgatgaa atcaccgtgg gcgggaccca acagggataa
    23581 ggatgcgcat cgaatgagcg gggcgttgca acagacggag gggtatatcc agttagcctc
    23641 cgatctcttg gacaaggcca aacgaagtgg cgcgacggag gccgatgtca tcattgccga
    23701 tggggaaaac ctggcggtgc aagtgcgcct gggagccgtc gatcgactca gcaaggccag
    23761 agaaaagcgg ctgggactgc gggtgtttgt cgggaagcgc tcggcctgca cctccacgtc
    23821 ggatttttcg gtggattctc ttaaccagct cgtcgcgcag acctgcacgc tcgccaaagc
    23881 ggtggtggag gatgccgtgt cagggttgcc ggctgccgat cagatggcga ccgaaaagcc
    23941 ggatctcgat ctatacgatg agacgaaact ggacacggat agacagatcg atctcgccaa
    24001 acgggccgaa gctgcagcca tgtccaccga cgagcggatt acgaactcgg aaggggcgga
    24061 tttcgaatct tcgtcgggcc gcgtcgttct cggcaacagt catggattta tcggcgaata
    24121 tcgcagttcc agtttttccc tttccgtctc tccgatcgcc acggatcctc agaccggtgc
    24181 catgcagcgg gatgcctggt atgcggtgca gcggaagttc gacaaactcg atgcgccaga
    24241 agcggtcggc atcgaagccg cccgccgaac agtcagacgg ctcggagcga ggaaagtcgg
    24301 caccaagcgt gtcccggtcg tcttcgatca ggaaatggcc ggcagcctgc tcgggaacct
    24361 gtgcggggct ctctcgggat attcgctcta taagggagca tctttcctcg ccggtcaact
    24421 gggcaagcca ctggcccctg actacatcac ggtctacgac gacggacgga tggtcggtgg
    24481 gcttggctca cggccgttcg atggcgaagg gcttgcgaca agaagaaaca tcattgtgga
    24541 gaagggtgtc ctcaagagct acttgctcga tacctactca ggaaggaagc tgggactgcc
    24601 ttcgaccggc aatgcatcac ggagtatagg ggagagccca tctgtcggcc cgacgaattt
    24661 ctatctggtt ccgagagaac agagcgcaga ggacattatt cgcaccgtga aggacgggct
    24721 gtatgtgacg gagttgatcg gctttggaat caacatggtt acaggcgact attcgcgagg
    24781 cgccggcggg ttctggattg aagacggcga gctggcgtat cccgtggaag aaatcacgat
    24841 tgccggcaac ttgaaacaga tgtttaagga catcgaaatc gtcggtagtg atctggtgtt
    24901 tcgcggacgc attgcctgcc cgaccctcaa gatcgcggaa atgatgattg cgggcaactg
    24961 acggagcgag agcgaataag atatggcttg tcgcgaattt ctggctatcg acgcgccata
    25021 tacatgcatg cacacacccg ctatgcattg atagcagcgg cgctcacagc tgccataact
    25081 gcaatggccg ccctagcttg tgaggagggc aacatgcgaa ttgtctcgac ggcgtttagc
    25141 aaccagagtg cgattccggc attgtatacc tgcgagggca aggacgtttc ccccccgatg
    25201 gcatggtcgg atctgccggt gggcactaag agcttagcgc tgattgtgga tgacccggac
    25261 gctccggatc cagccgcgcc gaaaatgacc tgggttcatt gggtgctcta taatttgccg
    25321 ccgacgagtg acgggctacc ggaaagcgtc aagacgctgc ccgcgggaac caaggaaggc
    25381 ttgaatgact ggaagcgtac tggctacgga ggaccatgcc cgccgatcgg ccgtcaccgc
    25441 tacttccata agctctacgc gctggacacg gtgctgccag acctgaagca gccgaccaag
    25501 gcaaagctcg aagaggcaat gaagggccac attattcgcg aggctcagtt ggtagggaca
    25561 tatcagaagg acagatgaaa ttcgtctgct cgcgaggtcg agcatcgggc tgtcacgtac
    25621 cggccgcccg ggcggaacac cgcaatccgc gggatgatcg cggggagtta aacaggcaag
    25681 agaggaaagg ttatgcgaag tgtatggagc tcgattcttt tgataatagt aatgcttctg
    25741 ataatagtaa tggctcccgg cgtaagcact gcaaccgatt ttcggctcat cagtcccaca
    25801 atcaaagata aaggcactat cggcaatgaa cacgtctaca acggtttcgg atgcagcggg
    25861 agcaatgcgt cgcccgagtt gcgatgggag cgagcaccta agggaaccaa gagtttcgct
    25921 gtgacggtct atgatcctga tgcccccacc ggaagcggct ggtggcactg ggtcatcttc
    25981 aacattcctg cgaataccac ctcgcttcca gcgggcgccg gcaagctgga tggcggagga
    26041 gcgccgtcag gcagcataca atccatgacc gatttcggtc agccaggcta tggcggtcct
    26101 tgccctccgc caggtgataa acctcatcgg tatgtcttta cggtgttcgc gctgaaggtg
    26161 gatcagcttc ctttgaacaa agaggcatcg ggtgcgatgg ccggtttcta tctgaatcag
    26221 aatgttttgg gaaaggcctc tttcacggcc aagtacggtc gttaataccg gatcaacgtg
    26281 gagtttatgg cgcgtcgcca ggcggaatgc tttgacactc ccctagacct ggtgtttata
    26341 gcgggaaacc tatcagatgc caagaccgct gaacgcgtac tcacggagca aggagtggat
    26401 tatgcaattg ctctcgagca atttaagcat gagtcggtac ttggcgcagt gtttggcaat
    26461 acgtataatg gcatattctt ctacgttcca tctgcagttc atagaatgac gatggatatc
    26521 ttggaaggag ctgggatgca ggatactgtc ccattgaatg cgggaaagag aaacgaggat
    26581 tgatatgccg cttgatgccg aatttggaaa gaaaatgctg cagttgatca cgtcgcgcta
    26641 tgacgatcgg cattggcgta agaagatcga aaagacgttg agtctgcccc aatcgggcgt
    26701 gagcgatccg atccagcagc agatctttat gtatctgaag gttggattga aggcctataa
    26761 gtctcgacgc gccgatcccg attcatggat catcggcggc tatgcgacca gagaagtcat
    26821 cgatcgcgcc aagttccaac ccaaggaagt tggctcagag atcgtcaagg atgatgtggc
    26881 gtttctcggg agcgatcctg gctccgaagt cgatgaggct tggtgggagg agatgttggt
    26941 acaatggttc gatgtgccgg aagaggagaa gcctgccgag gaagaaacag tggctgattc
    27001 agcggcatcg gcggaagcgg aagcttcgca gaagaagtag aggcctggcg tcacagccta
    27061 tcaaagcagc gggctctcta ttcgaataaa tccatctgcg caggatccgg cggttcgatc
    27121 tgaagcgtag gtatggttgc gggcgaggca tcggtcagtt ctgccggttt ggtattcctg
    27181 tccagaaatt ctttcagctt cctgaagtct tctttcagcg tgtcgagcag ccctccttgg
    27241 tgtaaggctg cagccgggtg aaggaggggg aagatcacaa agtccttcat atagaaggcc
    27301 tgcgccttca ccttcgtgat cccgaccttt ctttccaaga gtgtctgtgt cgcccaattg
    27361 cccagcgtgc aaaccaattt gggcctgatc atctgaattt gttgtaagag gaacggcttg
    27421 caggtctcca cttcgtccgg ttccggatcg cgattattcg ggggacggca cttgatcacg
    27481 ttggcgatgt agatttgatc gcgcgagagg tttgcagatt gcaagagatc gtttaagagt
    27541 ttccctgctg ctccaacgaa cggctcgccc ttttggtctt cgttaaatcc cggggcttct
    27601 ccaacgaaca tgatgctggc gtgtgggttg ccgacgccga acacgacttg ggtgcggccc
    27661 aacttagcga gcttgcattg ctggcagttg tgaagcgatt tggcaagttc ctggagggtc
    27721 atgggtgctg accgtagagt gaaactcgaa gggcatatta tggagggcaa ccgtgcttgt
    27781 caatcatgtg gaaccaagga ggcgtgtctg tctggacact cgttacgcga ccgtacctct
    27841 accatcaagc gcggagtccg ttatacgggt ttgcgaccat taataatctc ggcgcaacgc
    27901 tcaccgctga tgatggcact ttccaaaatg gcgggccatc ccgtgtcggt ccaggctccc
    27961 gcgagaagca aattcggaat ggggctcagt tgaatcggcc gccgggcctt tgtcccaggc
    28021 tggagtccca gcacagcgtg agccacctcg tgctgtctga agctggtcag ttggctatca
    28081 ggttgaagga gtgcgagcga cctgagaagc gcggagacct gttcctgtgt atcgatcgtg
    28141 gattgatcgg gaggtatggt cagcacggcg acgagactct tgtctgcttc ggagggctta
    28201 cagaccatcc agggaaaagg acccgttccc atcatgatgt gtcgagacgc atgaacggtt
    28261 tgtgcggtgc gaacctggat gaccgtgaag ggggttgtcg tgagttccgc aatttgctgg
    28321 aaatacgcat aacgggtgag ccaccgttcc ggcaggagtg gcgtgagttg gtggtgtgcc
    28381 actgccgcta tgtaccaatc cgcctggagt atcctcccgt cctggacgcg gacaccggta
    28441 atccgttcat gttcgaattg aaattgcatc gctctggtgt ccagtaacag cgtggcgccg
    28501 gcctgttgta gacggtcggc aaccggctgt acgaacatgt ggtgccacgg ttgtcgtgga
    28561 acacaaatac gattgtcggc tcctcgcgac agaaagatcg gcttcgctgc tgcgacaaag
    28621 gcatcggcgg agaggttgcg taggtcgttg cccgtgagcc attgggccaa gggatgccaa
    28681 acagtttgga tggtcgactg actgtgccca agcgattcga accagtcttg tgcggttcga
    28741 tgttcgagat cggcggcaag ttggagcgcg ccctcccaga gttgttcaag ccaggacagg
    28801 agcttccagc gttcccccca tgacagtgct gcaaactgcc caatggtgat gagttgatga
    28861 agcggcgcag gaagctggct tgtcggatag cgtgccaggc ggccgttcgg aagtagaaac
    28921 tccagcgagg cgttgtgaaa tgtggtcggc ataggagtga tgccgaggga gcgcaacaat
    28981 gaccatgttg catgatgaca acccaggatg gccaggggtg gatcgccctc acgcctgagg
    29041 tgttctccca aagccgacga ttggctcacc accgtcacgc gatggccctg aagggtcaac
    29101 cggtaggcgg cggtgagtcc cgccggccct gggccaatca cgatgactga tcggggggta
    29161 gagagtgtca cgagaatcga gagcgaagcc aaactccggc tgctacaacc agccggtgcg
    29221 aggtcgatag tcgggccctt ggaccaaaga tctgatggcc ggggagttca atccgtttca
    29281 ggatacgaga gtataacgct cgcatgattt ccgcgacggt cagcgcccgt tgatcgctgc
    29341 ctggcaggct ctccagtgcc acctgtgctt tggtatagaa ccgacgggct ctgtcggcct
    29401 caaagcggag aaggtcctga agctgagggc tgtctttgcg ttgaaggagg tccgactccg
    29461 tacagccgaa ccgggcgaga tcttcttgcg ggaggtagat ccgaccctgc tcggcgtccg
    29521 ctccaagatc gcgaaggatg ttcgtaagct gaaaagccat gccgaggtcg acggcataat
    29581 cctgggcatg aggtgaggtc gggccgaaga catgcaggca gatgagtcca acgatggatg
    29641 cgacacggta gcaatagacg gacagttcgt cgaaggtctt gtagcgggtg gtgaggaggt
    29701 ccatctcaac ccctttgatg agttcttcga aataggcttt tgggatcgag agctctcgtg
    29761 catggtgggc aaggctgatc gtgataggcc aagtcggggt accagcgtag acggcgtcga
    29821 gctcggcacg ccaacgccga agttcttctt tcgggttgct gtcgggcggg ggttcatcga
    29881 cggcgctgtc gactgccttg cagaacgcgt agatcgtgta catcgcgtca cgacgagtct
    29941 tcggcagaaa gaggaaggaa taatagaagt tactcccgct ctttttcgtc agattgacgc
    30001 agaaggcctg agcttcagtg gcggtcatgg tcatggagat ggactctcgg ccaaaggagt
    30061 gccggtgatg tcctgaacga caaatgcgtc gaactgagtg cgagctgatc cggagaatag
    30121 cgctgccaga agttcgaggc cttcgacgag acgcgggcca ggccgactga aataggaacc
    30181 ggcatcgacg acgtacgtgc gtggccagga tgccaatgca cgcggccagt ccttggagaa
    30241 cttgctgata gaagcgatct ccgccagcgt gcgctccacg gagaatccgc agggcatcag
    30301 gatgacgata tcggggtgtg ccacagcaac ttcattccac ccgacttcac gcgagggctg
    30361 attcatctgt ccgagcacgt ccactcctcc ggcgatctcg accatctcag gtatccaatg
    30421 cccgccgatg tagagaggag acagccactc gaaacaaatc acccgtggtc gtgccggcaa
    30481 ggcggcagag cgggcgcgga cggtcgcgat tcgctgacgg agcgattgtg cgaggtcgcg
    30541 tcctttcgaa ggctggccga gtgctgcggc aatccgctcc acatcgttta cgacgtcgtt
    30601 tagcgacgca gggttcagcg tgaccatccg aggtgcggct ggaaaagaac gcaggacgcg
    30661 acgtaactgg tctggggtca cggcgcagac atggcaaagg tcttgggcga ggatgacatc
    30721 cggttgagcc gccaacaagg ctgattcgtt caagcgatac agatgttgac ctgtcgaggc
    30781 caggtctttg acctgtcgat cgatgtctgc gcttcctaac ggttcgtggc cgacgactgg
    30841 ttccaccatg atgggtatgt gacgtacgga cgagggaaaa tcacactcgt ggctgatgcc
    30901 gacaaggtga tcggccaggc ccagagcggc gacgacttcc gttgctcccg gtatgaacga
    30961 acagatcctc atcgatcgat tattctttgg ttggaggcca taaacgggtc cggaggtcgg
    31021 cgaggctggc ttctttcaag ctgtgggtga ttgcgtgcgc ttcatggagg tcttgaaccg
    31081 tgtgcgtatt cgtcacggcg aggactttca tgccagcggc tttcgcactg cgaattccgg
    31141 gaagcgaatc ctcgatgacc aaacaggagg aggccgagag ggagggagtc cgctgctgca
    31201 gggccttcaa ggcatgcagg aacggttgcg ggtccggctt ccccttggtg acatcctcgg
    31261 cgctgacgat agagaaaaac tcctttcgca gaccggcttc ttccaagatg tattcgattt
    31321 catgccgtag cgctccagag gcgatagcca aaggataggc actggccgct tcacgcacaa
    31381 actcgtgaac gccgggaaag atcacgcgat gatgcttgac tgattcaagg taggccgcgg
    31441 ccttcctcgt catcagctgg gcgagagtgt tcggctcgat cggcctttga tttgcagtca
    31501 gggctgcgat aaaacatccg cgatcgtcgt atccgaggta gtccgcgtaa tactgggatt
    31561 cagtcagatc gattccgatc tcgctcaacg tctggcgcaa tcccgcaaaa tggagtggtt
    31621 cggtgtcggc aatcaccccg tcgaagtcga agatcagtgc gctgagcgtt tgcatagctg
    31681 gaagtctcag tcctcacgga aaataggacg atcaacggcg cggattatag catagaggaa
    31741 aaaaagagaa ggccggagat gtctccggcc ttccgcaggg aggaataaaa acaatggcgg
    31801 ttagaacttg aggcggagcc ggttttcgct gatccagcct tttgtaccgt cgtcggctcg
    31861 aactgagtag acccagccac ccggctgcag gtcgccgtcg aggacactta acgtgactcc
    31921 aggccgaacc gcaggacctg gtttggtgcc ggcgggatcc aatgtcaaag ggatcatttc
    31981 agctggaatc gtcgggttgc cgatcacggt gactttttgc ccttcgccga acatcggaga
    32041 tgagactgga cttgaggccg gtgccgcagg agttggtgcc ggagtcgacg cagagggcgt
    32101 cggtgcagga atctccggtg ccggagccgc ggcgctcgga ccaggcggtg cgggaacggc
    32161 gtcaacggga gccggttgtg ttgatggtgc tggaggagcc ggggtagtgg gcctgggtcg
    32221 catcgcgaca gcaggcggtt cagtgggcgt gttctctcca ataagggggg ccagccattc
    32281 cgccagttgc tccggctcca tcgcgatgta tccgattcct gcaatcaagg ctagaaggag
    32341 aatccacagt aatggccgac ccccgcctga tttctttctg ggtttcaaag gggaggtcga
    32401 tctggtcgag gtggtctggt cgagttcctc ttcggtgaat tccaagtcag gctccggctg
    32461 acgggcgaaa aagagcgacg attggatagg attcccgaca gctgtcaggt tccctgcgag
    32521 cgcagccatg agcatacctc cctcctgaac ggtcagagga atcacaagcg tacagtctat
    32581 ctgcagaact acttatcacc aaccttccgc actgtcaatt ttcctgaagt gttctgcggg
    32641 agtctgactc aacaaggctt tgcctgggag caggagagga cctgccgctc gcggcttccg
    32701 tcacgaaaga cggtaaggcc cttgcacctc aactgataag ccaggcgaaa cgcattggcg
    32761 acgtcagccc tcgttgcggt tgacggcagg ttgatggtct tggagacgcc gctgtcgctg
    32821 tactgttgaa agatcgcttg catgcgcaca tgatgggccg gcgacacgtc atgggcggtg
    32881 acgaagagcc ggcgtacgtc ctccggaatg cgggtcaggt gttggacgga ggggctcgcc
    32941 tgaagcgccc gttgaagcga agagagatcg agcccgcgtg ccgtgaccat acgtgcaaaa
    33001 gcgggatgga gtgtcgtcag attgagtcct tccatgactc tgcgcacgac cttgatgcca
    33061 tagagcggct caatgccggg agaacagccc gcgatgatac taatggtgcc ggtgggggca
    33121 atcgtcgtga ccgtggcgtt ccgacgaggc cgatgctgcc tttgaagctg actgccgaga
    33181 taggccggga atagtcctcg ctgcacggca agctggtgag aggcctcatg ggcgtgagtt
    33241 tggacgaatg ccattaactc accggctgtc tcgatcgcgg cctcggagtc gtacggaatc
    33301 tttagggcga tcaggagatc agcgaagccc atgatgccaa ggccgatttt tcttgtgcgc
    33361 cgggtggcgg tttcaatctg agcaagcggg taatggttcc gatcgatcac attgtcaaga
    33421 aagcggacgg cgagttcaat cgtgccggcc aatttcgggt aatcaatcct cggttgcgtt
    33481 gggcttgtca gaataaattt cgatacgtcg atggatccca acgtgcagga ttcgtggggc
    33541 agcagcggct gttctccgca gggattagtc gcttcgatgt cgccgagttg gggcgtggga
    33601 ttggcagcat tgatcgtatc cagaaagatc actcccggtt ctccggtcgc ccaggcggcg
    33661 tccacgagcc ggtcgaagac cagttgcgag gagaggcgct tggccggttt gccggtccga
    33721 ggattgacga ggggataatg ttgacggcga tggagggcgc gcatgaattc cgccgtgagg
    33781 ccgaccgaca agttgaagtt tgtcatttca gtcgggtcct gcttcagcgc gataaattcc
    33841 agaatatccg gatgatcgac gcgcagaatg cccatgttcg ctccgcgcct cgctccgcct
    33901 tgtttaatga catccgtggt cagattgaag atgcgcatga aggagatcgg tccggaggcg
    33961 atgccactgg tcgaggcgac gaggtcccgt tgcggacgaa gatggctgaa ggagaatccc
    34021 gtgccacctc ccgattggtg aatgagcgcc tgttgtttga cggcatcaag gatggaactc
    34081 agcgaatctt caaccggcag gacaaaacag gcggccagtt gttgcagcgg tcggccggcg
    34141 ttcatcagcg tgggggagtt ggggagaaat tccagccgga ccatcgattc atagaatcgg
    34201 cgtgccgcct ggggcagccg cgcgcgggac gcatagccct gctcggcttg tgcgatatcg
    34261 gttgcgattc gccagaggag ttgcggggca gtctctgtga ccacgccgcg ggaatccctc
    34321 gcgaggtacc gccgccggag gacaatttta gcttgcgggg aaagccgggg aacaggggat
    34381 gccggaagtg gggtcagccg aggcgaaaac gtcatggcct attatagtct ccgcgtcggt
    34441 gaatgacgat atgccttcaa cgtgatatcg gacttgaatc tctgtccctg cgggaagcag
    34501 aatgcaatcc ctggacttgg cgccatgaga tcctaccgtg aagaactctg gttcgaaacc
    34561 acaacgcgtc gcgcgtacat caatatcacg ccgcatgtcg aggcggtggt aaaaaagagt
    34621 ggtgtcaatg aaggactggt gctggtcaac gcgatgcaca ttaccgcgag cgtttacatc
    34681 aacgatgacg aacaggggtt gctgcaggac tacgaccgat ttttggaagc catcgcacca
    34741 cacaaggccg cttatcgcca caatgagacc ggggaggaca acggagacgc ccacatcaaa
    34801 cggcagttga tgggacgtga agtggtggtg gcgattacgg acgggcgtct ggatttcgga
    34861 ccttgggagc agatttttta cggagagttc gacggacgcc ggcgcaagcg cgtcctggtg
    34921 aaggtcatcg gagagtaaac tgtgacgatt cggtctgtct cggaatcggt attcacgcgc
    34981 aaaccggtcg agcgcttgct cctgggggaa aagtttggca acatgaagga atggattgat
    35041 cgttagtccg cccggagtgt cgcatgcaaa gttggtctcg ccctgatcct ctgaccagag
    35101 atttgatcca tctgatcaat gcgcgccggc acgaccacgg cgacacggat gccgcgatcg
    35161 acacgctgca atcctttctc gagcagggac gggaacacga tctcttgacg gtcttggccg
    35221 cactcgatga agacatggcg gagtggttgt tcgacctggt tgcggaagcc acctgttcca
    35281 tcctccttga tggcccggtc gacgacaagc tgtcgtatgc cgttatggcc gcactggaac
    35341 ttccgttgca ggcgaatctc acgcatccct tgaaattgaa gatcgatccg gttgcgacag
    35401 cagacatcct ccacaggcat ctccgcttgt cggccggaac cgtcgttcgc gtagaaccac
    35461 gactattgac cgacgccaca cttgaggcgt tgactctgca agcgttaaac gatcatctcg
    35521 gcaccgttgc ggattcccag cgggcccgtt cgatcgctgc ccgtttatat ccgccggtcg
    35581 agaataccgc ctttctcttc tttgagttga tcggtgtgcc ggattcgggt ttgcccgatt
    35641 ccctcgaaga tccgcatcgt cgcgcgctct tggagggatt ggtggagcaa tgtcccgaat
    35701 tagcgttggc gccggatacc ctggaagctc ccgtctatgc cgcctacgcc atgttcgacg
    35761 cgcaaaatgc tgtcaggctc catcgtctgg ccgatgagac ggcggctgtg tggagagatc
    35821 ttgatccact cgtgcgtgcc cggacgattg ttcatctgca tacccaatgc gggaacggag
    35881 tctacatgcc ggtatggacc gatttgttcg atcctgaatt gccggatgag catggtccgg
    35941 tctggacctc acctgacgtc tgggtcttca ccgcagacct gccgattgaa tggccgggag
    36001 agatgctgac gaatcgattg ctggccgaag gctgcaccag gttcgagaat catattgtcg
    36061 aagctccgga aaactgaatc cttcctttct cttccttacc gacccgctgg caactttgat
    36121 gttcaaggcc tgcggctatc tgcaaattcc tcatattccg tcgatttttt ggggaccttg
    36181 ccctatcctg tgggctggaa ttgtaaggag gaattatggg gcaaccacat tcgtctggcc
    36241 gtatcgtcgc tggggtcgtt acggttgtcg gacttggcat tgtctggctg gccatcctca
    36301 tgagccttcc atcgcaacag gagctttccg cgaaagaacc gaagctatcg cagcaaccaa
    36361 tttcagcttc taaggcgcaa cttccttccg agccgacgtt ggatctttcg attccgggtg
    36421 tgccttctgc tccgccggct ccttccgatc aggatcctgc cgcaactgtc ggcgggtcgt
    36481 tatcaccccc
//
LOCUS       DATIFR010000023        14387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_203694, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000023 DATIFR010000000
VERSION     DATIFR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14387
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203694"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VLD60_01070"
     CDS             <1..494
                     /locus_tag="VLD60_01070"
                     /inference="COORDINATES: protein motif:HMM:NF042722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6635 family protein"
                     /protein_id="HSB43568.1"
                     /translation="LLASGALSAEALSDLSDIPRLVGEHSSSRALVLDTAATVLTLAA
                     GWLFFGDHSLGISGIGDHIARQRAKDRAASTFILGSSLGSAFYSVFPPKPSFWQVVGA
                     TFTVGLFITGMSLLVGLVSDPCLKRAGFQHARLHLLIDAVEDCLHRQLRTRLKPAIRQ
                     LAA"
     gene            1085..1423
                     /locus_tag="VLD60_01075"
     CDS             1085..1423
                     /locus_tag="VLD60_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43569.1"
                     /translation="MWMVWLLGILLFAAAASAEPGFSEKYERDYNIFHSINQYRSDNP
                     LNPISAYDLTNPFNSINQYDPGNPANPINQYNLNNPFNPINQYNLQNPLNPINEYDPA
                     TPFQPLNRSR"
     gene            1487..1696
                     /locus_tag="VLD60_01080"
     CDS             1487..1696
                     /locus_tag="VLD60_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43570.1"
                     /translation="MTHVIDFDKVTTVLLQHGEWYEVDPGTFTSIPAANFLSGHSGSN
                     AYAWKSHGASLACPCSALVLVRYSM"
     gene            complement(1760..2014)
                     /locus_tag="VLD60_01085"
     CDS             complement(1760..2014)
                     /locus_tag="VLD60_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB43571.1"
                     /translation="MASKIHISGLPFFFTNAQLRRIFVPFGNVESAQVLKSAYGHSLG
                     VVQMSSPEEVEQIFSAQQLFEVDGKHLDIWEPADPEYTQI"
     gene            2525..2692
                     /locus_tag="VLD60_01090"
     CDS             2525..2692
                     /locus_tag="VLD60_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HSB43572.1"
                     /translation="MLYYALVFLVVGLIAGALNLAGVSSVAVQISWILFLIGIVLVAV
                     HMVTGRSARVA"
     gene            2772..2993
                     /locus_tag="VLD60_01095"
     CDS             2772..2993
                     /locus_tag="VLD60_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSB43573.1"
                     /translation="MNADQLTGQWLQFKGELKRQWGKFSDDDLQQIAGNYEKFIGKVQ
                     ELYGEKKDELMKWADRWHEKSEPHAVGKK"
     gene            3069..3626
                     /locus_tag="VLD60_01100"
     CDS             3069..3626
                     /locus_tag="VLD60_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB43574.1"
                     /translation="MQTRTVLTFCAALMIGGALLTGSANALGKADEKTPGNDAYVTAK
                     TKIALFADARVKGSEINVETNQGAMMLRGKVDSDTAKQAAEGIAKGIDGVKSVKNDLQ
                     VVAPAKREAIDDKDEAITKRVNEQISKDPSLTKAGIHAQTNAGVVSLSGEVQDLMSSA
                     QASSIAWQVPGVKSVKNDLTVKEKA"
     gene            3747..3986
                     /locus_tag="VLD60_01105"
     CDS             3747..3986
                     /locus_tag="VLD60_01105"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB43575.1"
                     /translation="MVRETVSNPTTPRVSAGTSKVGPNAEESGPAIRISKADWDHERV
                     ARRAYELYEQRGRQDGRDLEDWAKAERQLAGASSQ"
     gene            4044..4520
                     /locus_tag="VLD60_01110"
     CDS             4044..4520
                     /locus_tag="VLD60_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSB43576.1"
                     /translation="MNTLLRWDPITRTRWNPFKDRDELESRLATMFATREATGNGGKE
                     ALTVAQWSPLVDITEDEKQYVIKAELPEMKKEDVRLTVENDVLTISGERKFEKEEKGR
                     KYHRVERAYGSFVRTFSLPEDADGSKVTADYKDGMLQVHLPKSEKAKPKAIEIKVG"
     gene            4721..5155
                     /locus_tag="VLD60_01115"
     CDS             4721..5155
                     /locus_tag="VLD60_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB43577.1"
                     /translation="MSEFKSGFFTGSEACNGRVLVVDDEPDIRTVVRLSLEKGDYEVL
                     EAENGEQAITIINDGENRLLLDVVMCDVRMPKINGIEAIAYFQANYPRVPLIVLTGFP
                     DTGMAAALFRQGVIDYLVKPIESEALRASVARAMDRREPARL"
     gene            5152..6321
                     /locus_tag="VLD60_01120"
     CDS             5152..6321
                     /locus_tag="VLD60_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB43578.1"
                     /translation="MTTTSTAMGPAGNLEGRTALVFSPLTDVLPKHADGNTNGTVAAP
                     ATNVAIVGAGRGGTALLELLHQIPSIHIVGIADRDPLAPGLQRARELRIPVCDTISTL
                     IGHHHVNLIVDVTGDPEMERSLAGHKGLTVEIVSGSVSRLLRKLVQHESRLHSELIQV
                     DKLAGIGSFAAGIAHDINNPLQLIMGLAENLVDEQNPAVVREHSKEIIEAVKRTSAIC
                     CDLTQYARPASMPGDTVVCLNDRLDEALKIARYAASFHDNDVVKAYTPGVAVRGNPDE
                     LLHMFVNLIINAIHSMDHSGGTLTLATFLSGNGAGVRITDTGCGIDPDTSGQIFEPFF
                     TTKERGKGTGLGLYNVKTMVEKMHGTITVESEADRGTTFTLTFPANDPSGGSGAS"
     gene            6627..7310
                     /locus_tag="VLD60_01125"
     CDS             6627..7310
                     /locus_tag="VLD60_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HSB43579.1"
                     /translation="MVLVPYIDVLSAAFLMGFAAAAPMGPVNMLAIRRGVIGGWRHTL
                     ACGMGSIAGDLILFSVVLLGGDYFLPDLANPTFRAVLAATGVIVLFPLGIYFVFRAVK
                     EPLRAYASACERWDEGTVRGHLIAEVADAAAMTVFNPLTIAYWIGVTANWLPFAHSVL
                     GYSAPGFGIVMVTAGLMTWFTALTVIVRFVPHRIGPNFFRLVNAVLSLILLGFATFCA
                     IILSRHLLY"
     gene            7481..7783
                     /locus_tag="VLD60_01130"
     CDS             7481..7783
                     /locus_tag="VLD60_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43580.1"
                     /translation="MQMVMMVFRTSLEHDLLPWIEQEELPFTRIDSALGKGATGNIPG
                     RATFGGSNSILLLAIPDERLASFRDRAHQFHDELLGRHKGVGTPFHTFILPCIQWF"
     gene            complement(7822..9789)
                     /locus_tag="VLD60_01135"
     CDS             complement(7822..9789)
                     /locus_tag="VLD60_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HSB43581.1"
                     /translation="MKDDQPVVPATVTLPEITVKAVVLSVILAAILAAANAYLGLFAG
                     MTVSASIPAAVASMAILRLFRRSNILENNIVQTAASSGEALAAGVIFTIPALLLVGYW
                     SDFDYWQTASIATVGGLLGVLFTIPLRRALIVTARLRFPEGVATAEVLKVAAPDRRNA
                     APAESTSDFRALLSAALLGGAVKFGESGVRLWAESLEGAAQVGRTVFYAGVNLSPALL
                     AVGFIIGLQTAVVVFLGGVVGWMVLMPAYGFLYGLPPDLTGLAAATTIWSGQIRYVGI
                     GAMLTGGFWTLTMLREPLWRSLQTLRESYRPAGTSRSAAVIARTEQDASLPWIVVPFG
                     LSLIPMAWIYTSVVNSPLIGLLMTIVMVLAAFLFSSVAAYMAGLVGSSSNPVSGVTIA
                     TIMCAALLLVLFMGAGHPAGPAAALVIGAVVCCAAAMGGDNLQDLKTGHLVGATPWKQ
                     QIMQVVGVLTGAVVLVPVLSLLQAKYGIGEPTISHPHPLSAPQATLMASLTRSVFGAG
                     LPWPLVGLGMAIGIIVILVDRRQEARGRDFRLPVLAVALGIYLPLKLSAAIFVGGVVS
                     ALARRATAKGDDASRRGLLFSAGLITGEALMGIMLAIPIALAALWPNLSPDPFMLFES
                     PPFGGWPGLIIVAMVGCILYLIGRGPMRKKR"
     gene            complement(9987..10442)
                     /locus_tag="VLD60_01140"
     CDS             complement(9987..10442)
                     /locus_tag="VLD60_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HSB43582.1"
                     /translation="MRCVECFFENPSGVRRCRICRATLPDDTAPASSPPSTETLAIDT
                     RHRRGGFFSFGTLITPAAIQVAYIIGAVLITLAGVLMVTLVLTGVASEYTDTNRDALL
                     FGGLTVLGIGNLLWRVLCEMGMLLFRILEALLELDDKAHSLIRLLAERK"
     gene            10668..11294
                     /locus_tag="VLD60_01145"
     CDS             10668..11294
                     /locus_tag="VLD60_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43583.1"
                     /translation="MTCLRQLGAIVFLSGILAGCTDGVVKPVGLDVALRGVEEDMKAA
                     SGVSMHDILSGDAAQEEEFKQAIRQEQCFYRRPNPLVPVINKEFTLTLQGTFTGQGKF
                     LVLGIPVPAGGVEISAAKTLQQTLALPVNFTSVSSLPDVYLQQKASYVKDFPESEKAM
                     YLEEVFKERDVIRASIKELIASYSEERCRRMPPDPLSKNHPVAPRPAH"
     gene            11701..12009
                     /locus_tag="VLD60_01150"
     CDS             11701..12009
                     /locus_tag="VLD60_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HSB43584.1"
                     /translation="MITLDEIRQRLVAGTFEFSRHAFKRAVERNISERDIREAGRRAA
                     IIEDYPDDKYAPSCLILGFTDAGHPLHIQVSYIESDMLKIITIYQPDPEEWYDYSRRR
                     "
     gene            12012..12245
                     /locus_tag="VLD60_01155"
     CDS             12012..12245
                     /locus_tag="VLD60_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HSB43585.1"
                     /translation="MFRCHVCGKTEARQELVSEVFDIDDKPVRVEAIPAIVCANCGEA
                     VFSRETTERVRRMVHGEAKPVKSVQMDVFAYTK"
     gene            12520..13542
                     /locus_tag="VLD60_01160"
     CDS             12520..13542
                     /locus_tag="VLD60_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HSB43586.1"
                     /translation="MVTPMSVLAPFLAGLCLVALPVSTHSVQPEPDRGVWRTAAPAPT
                     KRTEVAAATLNGKIYVVGGFEQPGLGNLLNLAITASVQEYDPSTDRWTTKASMPVGLH
                     HVGIGVTGGRLYVIGGYTQSGLTVWHPVATVYAYDPATDSWAERATMPTARGALSVTV
                     HEGKLYAIGGYDRNANSAAVEVYDPERNAWTSRAPLPTPRDHLASATAAGKVYAIGGR
                     VNGDYRRNLSVMELYDPVTNRWARAADLPTARSGITAAEVGGRIYVFGGEGGGGTFRE
                     NEAYDPVRDTWQTMAPMPTGRHGLGSAVVQGRIHVLSGGPTPGGSFSDLNEVFTPPGE
                     RMTKEG"
     gene            13552..14214
                     /locus_tag="VLD60_01165"
     CDS             13552..14214
                     /locus_tag="VLD60_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HSB43587.1"
                     /translation="MRRTPRNSVVVGVDVGGVKKGFHAVALRDGQFFGKLNTLDAVAV
                     IQWCRSLRASAVGIDAPCCWSLSGHARPCESALAMEGIHAFATPSRAVGGRHPFYGWM
                     RKGDALYRRIAHHYPLFDGTLPTSRPVSFETFPHAVGCALAGTILSGKRKRIDRLKLL
                     RETGLSTESLTNIDQIDAALCAVTARYLLAGNVNMYGNLKEGFIVVPKIERSSRKPAS
                     IF"
BASE COUNT         3071 a         4077 c         4337 g         2902 t
ORIGIN      
        1 cgttactggc gtcgggcgcc ttgtcggcag aggcattgag tgacttgtcc gacatccccc
       61 gcctggtcgg tgaacattcg tccagccggg cactggtgct ggatacggcg gcgacggtgc
      121 tgacgctggc ggccggatgg ctgttcttcg gcgatcactc gctcggcatt tccgggatcg
      181 gggatcacat tgcgagacag cgggccaagg accgggcggc ctcgaccttc attcttggat
      241 caagtctggg ctcggcgttc tattccgtgt ttccgccgaa gccgtcgttc tggcaggttg
      301 tgggcgcgac tttcacggtg gggctcttta tcacggggat gagcctgctg gtgggattgg
      361 tgagcgaccc ctgtctgaag cgtgcgggct ttcagcatgc acggctgcat ctcttgatcg
      421 atgccgtgga ggactgcttg catcgacagt tgcgcacgcg gctcaagccc gccattcgac
      481 aattggctgc gtaagacaag gggatcaggt ccagcaaccg aacagccgct tggctgccgt
      541 ccatttacgc tccggccgct cggccgtctg ttcatccgaa acgacgtcgc accccaacat
      601 gcggacacaa agacgacgtg aaacatgatg tcgctccacg gaccgaagag gtagccttca
      661 tctgcgccgt gggcgtaggc gagcaagagg gtcagtgcgc cgaccgacag ccatcgggct
      721 ccggtgatcg ctgccgactt tcctggtcgg agaaacagca gacccccggc ggacacgaga
      781 cttccgagca gagttaagtc gaatgtgctg atcatggagc gctcctcgcg ggcgatatac
      841 caccagacga gtctttctca gtgctgaacg ttcagaagct gagggatcga tcggcgggaa
      901 tgtggtggta ggcgttacga catacgactc acgatttctt cacgacggcc atgccctgga
      961 cggggggtac ggtacaacaa gcgaatagct gattcaagag ggcacgatgg ggccagcttg
     1021 cacgaatccg ccatccggga gtaggctgag cccagtaatt atcccgctca tcccggggtt
     1081 cacgatgtgg atggtctggc tcctcggtat cctcttattc gccgctgccg cttcagctga
     1141 acctggcttc tccgagaagt acgagcgtga ctacaatatc tttcattcaa tcaatcagta
     1201 ccgctccgac aatcccctga atcccatcag cgcgtacgat ctgacgaatc cgttcaactc
     1261 gatcaatcaa tatgatcccg gcaacccggc gaatccaatc aatcagtaca acctgaacaa
     1321 tcccttcaat cccatcaacc aatacaatct gcagaatcca ctgaacccga tcaacgaata
     1381 cgatcccgcc acgccctttc agccgctcaa tcgctcgcgg tgaggtgagg agggtgtgag
     1441 gatcagaaca ggtaccggga gtatgttctg tgggaggagg tgagccatga ctcacgttat
     1501 tgatttcgac aaagtcacca cggtgctgct gcagcatggc gagtggtatg aagtcgaccc
     1561 cgggacgttc acatcaatac ccgccgcgaa ctttttatcc ggtcactcag gaagcaatgc
     1621 ctatgcgtgg aaaagtcatg gggccagttt agcctgccca tgcagcgcgc tcgttctcgt
     1681 ccgctattca atgtaaagct tccggccttc cggggctttc cttggaccca tctccacttc
     1741 tctctcgttg cgcggggagt cagatctgtg tgtattccgg gtctgcaggt tcccagatat
     1801 ccagatgttt gccatccact tcaaagagct gttgcgcact gaagatctgc tccacctctt
     1861 cgggtgagga catttgcacg accccgagag agtggccata cgcactcttg aggacttgcg
     1921 cggactccac attcccgaac gggacgaata tccgacggag ttgcgcattg gtgaaaaaga
     1981 aggggagccc gctgatatgg atttttgatg ccatgcctaa tcctccttgt gagaggtagg
     2041 cattgtcttg ttgagcaggg gcaggaaaat aagcccgaga gtgaggcccg aaacggggct
     2101 tgtttaggag ctcttgcgca aggcaatgtc tgccttaacg gtgatcgaac tacttttacc
     2161 ctacacgtaa gacgccagca aagcaaccag tctcgtcatt cgagcgtagg acaatagcct
     2221 tcgtactgtg aggcgatctc tccgtgattg acagatccag gccttcatta tactgcttga
     2281 ctctcatgcc cgatgcgtcc agggtgaagg tgtccggtgt gaagctccgc tgactgtcct
     2341 atctcttgcg cttcctgctt cttcccagcg tgaccgattc cccagtctcc gccgctccga
     2401 tgccagagtg tctctgaacg gcgcatgccg tgtgttgtgg tctgcttgat ggagcggcca
     2461 ggatgccggc tctgctcgag acacccattt gactgatgtc ccattccata gagaggagtg
     2521 agccatgctt tactacgcac tcgtgttcct ggtcgtcggc ttgattgccg gcgcgttgaa
     2581 tctcgccgga gtgtcctcgg tggcggtcca gatttcctgg atcctgttcc tgatcggaat
     2641 cgtgctggtc gcggtccata tggtcacggg acgcagcgcc cgggtggcgt gaccggcgtg
     2701 gtggggtggt ccgccggttt ccggtgtgat gacctgcccg aagttaatct gtcgagagaa
     2761 aggagaaaac aatgaatgct gatcaactca ccggtcaatg gttgcagttc aaaggtgaac
     2821 tgaaacggca gtggggcaag ttttcggatg atgacctgca gcagatcgcc gggaactacg
     2881 aaaaatttat cggcaaggtc caggaactct acggggagaa aaaggacgag ctgatgaaat
     2941 gggcggatcg gtggcatgag aagtcggaac cgcacgccgt gggaaagaag tagtcccgct
     3001 gcatggtagc gaagcacaca agacgactgt cgacacactt ttactttatt gtggttaagg
     3061 agaagataat gcaaacacgc actgttttga cattctgcgc ggctttgatg atcggtggag
     3121 cactcctcac gggttcggcg aatgcccttg gcaaggccga cgagaagacg ccgggcaatg
     3181 acgcgtatgt gacggcgaag accaagattg cgctgttcgc cgatgcgcgg gtcaaaggga
     3241 gtgagatcaa tgtcgagacc aaccagggcg caatgatgct ccgtggcaag gtggattccg
     3301 acacggcaaa gcaggcggcg gaagggattg ccaaagggat tgacggcgtc aagagtgtga
     3361 agaatgacct ccaagtcgtc gcccctgcga agcgcgaggc gatcgatgac aaggacgagg
     3421 cgattacgaa gcgggtcaac gaacagattt cgaaagaccc ttcgctgacg aaggccggga
     3481 ttcatgcgca gacgaatgcg ggtgtggtct cgctgagcgg cgaggtacag gacctcatgt
     3541 ccagcgccca agcgtcttcg attgcctggc aggttcccgg cgtgaagtcg gtgaagaacg
     3601 atcttacagt gaaagagaag gcctaagtcg ttcctcataa aacaaaggcg ctctccgtcc
     3661 gtcgatccgg atcagtggta tcgacggacg acagagttgc tcactcagtc aaccacgaac
     3721 cacacggcag cgtgaggagg tgcacgatgg tcagggaaac tgtttcgaat ccaacaacgc
     3781 cgagagtgtc ggccggcacg tccaaggtcg gtccaaatgc tgaagagtcc ggtcccgcga
     3841 tccggatatc gaaggccgat tgggatcacg agcgcgttgc gcggcgggcc tacgaactgt
     3901 atgagcaacg cggtcggcag gacggccggg acttagagga ttgggcgaag gcagaacggc
     3961 aactggctgg cgcatcgagt cagtaatcac acacaccggc ggtcgaatgg atttccattt
     4021 cgatcaccaa aggagatgag gctatgaaca cgctcttacg ttgggacccc atcacgagaa
     4081 ctcgatggaa tcctttcaaa gatcgggatg aattggaaag ccggttggcg acgatgtttg
     4141 ccactcggga agcgacgggg aacgggggaa aggaagctct gacggtggcc cagtggtcgc
     4201 cgctggtcga cattacggag gatgaaaagc agtacgttat caaggccgaa ttgcctgaaa
     4261 tgaagaaaga ggacgtgcgg ctgacggtgg agaacgacgt cctgaccatt tccggcgaac
     4321 ggaaattcga gaaggaagaa aaggggagaa aatatcaccg ggtcgagcgc gcctacggaa
     4381 gcttcgtgcg caccttctcc cttccggagg atgcggatgg gagcaaggtg accgccgatt
     4441 ataaagacgg catgctccag gtgcatctcc cgaaatccga gaaggcgaaa ccgaaggcca
     4501 tcgagatcaa ggtgggctag tgttgcgttg attcgggggc gtcagggaag acttcgtacg
     4561 cagcagcgac gatggggtct tgctgacgcc ccgtgcccgt tactcccacg ttataggttc
     4621 tcactcgcgc ccgcctgctg gacagaccag caaagatccc gcatgagcat cccgcgtttc
     4681 tgatgctcta gaggaatact ttaactacac ggaggtcgtc atgagtgagt tcaaatcagg
     4741 attttttacc ggaagcgagg cctgcaacgg gagagtgctc gtggtggatg acgagccgga
     4801 catccggacg gtggttcgac tgtcgctcga gaagggcgac tacgaagtgc tggaagccga
     4861 gaacggcgag caggcgatta cgatcatcaa tgatggcgag aaccgtctcc tgctcgacgt
     4921 cgtgatgtgc gatgtccgta tgccgaagat caacggcatc gaggcgatcg cctatttcca
     4981 agccaattac ccgcgtgtcc cgctgattgt cctgacggga ttccccgaca cgggaatggc
     5041 cgcagcgctg tttcgccaag gcgtcatcga ttaccttgtc aaaccgatcg agagcgaggc
     5101 gctcagagcc tcggtcgccc gcgcgatgga tcgacgcgag cctgcccgct tatgacaacc
     5161 acctccacag cgatgggacc agccggcaac ctggagggga gaacggctct cgtgttctcc
     5221 ccactcacgg acgtcttacc caagcacgct gacgggaaca ccaacggcac ggtcgcggct
     5281 ccggcgacaa acgtcgccat tgtaggggca ggtcgcggag ggacagcgtt gctggaactg
     5341 ttgcatcaga tcccctccat ccacatcgtt gggattgccg atcgcgatcc gctcgccccg
     5401 ggacttcagc gcgctcgcga gttgcgcatc cctgtctgcg acacgatcag cacgctgatt
     5461 ggccatcacc atgtgaatct gattgtcgac gtgaccggcg atccggagat ggagcgctct
     5521 ctcgctggcc acaaagggct gacggtggag atcgtaagtg gctcggtgtc ccgtctactc
     5581 cggaaactgg tccagcacga atcccgcctc cacagtgagt tgattcaagt ggataaactg
     5641 gccggcatcg gctcgttcgc cgccgggatc gcccacgaca tcaacaatcc gctccagctg
     5701 atcatgggat tggcggaaaa cctggtcgat gagcagaacc ccgccgtcgt ccgcgagcac
     5761 agcaaagaga tcatcgaggc ggtcaagcgg accagcgcca tctgctgcga tctcacgcaa
     5821 tatgcccgcc cggcctccat gccaggcgac accgttgtct gcctcaacga ccggctcgac
     5881 gaggcattga agatcgcccg ctatgcggcg agcttccacg acaatgacgt ggtcaaggca
     5941 tacacccccg gcgtcgccgt ccggggcaat ccggatgaat tgctccacat gttcgtgaat
     6001 ctcatcatca acgccatcca ctccatggac cacagtgggg ggacactcac gctggcgaca
     6061 ttcctgtccg gcaacggggc cggcgttcgg atcaccgata ccggctgcgg catcgatccg
     6121 gacacgtcgg gccagatttt tgaaccgttt ttcaccacca aggaacgtgg aaaaggcacg
     6181 ggactcgggc tctacaatgt caaaaccatg gtcgagaaga tgcacgggac gattaccgtc
     6241 gagagcgaag ccgatcgcgg cacgaccttt acgctcacct ttcctgcgaa cgacccatcg
     6301 ggaggaagcg gtgcgtcatg agtcctcctc cgttcgcccc gaccgtttca ccaacgctca
     6361 tgtcagctcc gcaatctgag accgttccaa aggagaaggc cggaccacct atgaactcgg
     6421 cgcgagagaa gaaaaaggta ccgaggtcgt ttgctgggaa ttgacaagtc cttgaggccc
     6481 tacgagaatc agttccgttc atgccaccga agcggatcga actatcgaaa tagaaaaagg
     6541 ggtcgcgagt ctgttctcgc cgcctcattc tctgtacaca cctcttgaca caacttgttt
     6601 ctgggttacc ctagcgctgt cgcaacatgg tgcttgtccc ctacatcgac gtgctttcgg
     6661 ctgcgttctt gatgggattc gcggcggctg cgccgatggg tcctgtcaat atgctggcca
     6721 tccgccgtgg tgtgatcggc ggttggcgcc atactctggc ctgcgggatg ggctccatcg
     6781 ccggcgatct catcctcttt tctgtcgtcc tcctcggggg tgactacttc cttcctgacc
     6841 tagcgaaccc gacgttccgg gctgtcctgg cggccacggg tgtcatcgtc ttgtttccgt
     6901 tggggatcta tttcgttttc cgcgccgtca aggagcccct gcgagcctat gccagcgctt
     6961 gcgaacgctg ggacgaaggc acggtccgcg ggcatctgat tgccgaagtc gccgacgctg
     7021 ccgcgatgac cgtcttcaac cccttaacca tcgcgtactg gattggtgtc accgccaact
     7081 ggcttccctt cgcccattcg gtcttgggct acagcgcccc cggttttgga atcgtgatgg
     7141 tgaccgccgg cctgatgacc tggttcacgg cgctcaccgt catcgttcgc ttcgtgcctc
     7201 accgcatcgg cccgaacttt ttccgcctcg tcaacgctgt cctcagcctt atcctccttg
     7261 gctttgccac cttctgtgcc atcatcctgt cccgccatct cctgtactga gcgagcaatc
     7321 cggagacata cctctcccca tttcttgagg cctcgggaga ttacttgcgc tgtcctacag
     7381 gcctggagta ggctcagctc ctttgccgct cccgcttttc gcaggaacgc gatgtgggat
     7441 cggatatggc attcaccccc agctccatga ggcgacgcct atgcaaatgg tgatgatggt
     7501 attcaggact tcgctcgagc acgacctgtt accgtggatt gagcaggaag agctgccatt
     7561 cacgcgaatc gatagtgcgc tgggtaaagg tgcgacgggc aatatccctg gcagggcgac
     7621 gtttggcgga tcaaatagta ttcttcttct tgctattccg gatgagcgct tggccagctt
     7681 tcgagaccgg gcgcatcaat ttcatgacga actcttgggt cgtcataaag gcgttgggac
     7741 tccgtttcac acgtttattc ttccctgtat ccagtggttt tagtgcgttg tcccttttca
     7801 ggctggctcg tttgtccttt ctcatcgctt cttccgcatt gggcctctcc cgattaaata
     7861 gagaatacat cccaccattg ccacaatgat gagaccgggc cagccgccga acgggggcga
     7921 ctcaaaaagc ataaacggat cgggactaag gttgggccac agtgcagcca gcgcaattgg
     7981 gattgccagc atgatgccca tcaacgcttc accggtgatc aggccggcag agaagaggag
     8041 tccacgccgc gacgcatcgt cacccttcgc tgtcgctctt ctggccaggg ccgagaccac
     8101 gccgcctacg aaaatggctg ctgagagttt cagcggcaag tagatgccca gggccacggc
     8161 gagcaccggc agacgaaagt cgcgtccgcg cgcttcttgc cgtcgatcca ccagaatcac
     8221 gatgatgccg atggccatgc ccaatccgac gagcggccag ggcagcccgg ccccgaacac
     8281 gctccgcgtc aggctggcca tgagtgtggc ctgcggtgcg ctgaggggat gggggtggct
     8341 gatcgtcggc tctccgatgc cgtacttggc ttggagtaag gacaggaccg gaaccaggac
     8401 cacggcgccg gtcagcaccc cgacgacctg catgatttgc tgcttccacg gtgtcgcgcc
     8461 aacaagatga ccggttttca agtcttgcaa attgtctccg cccatggcgg cggcacaaca
     8521 gacgaccgct ccgatcacca gcgccgccgc cggtccggcc ggatgcccgg ctcccatgaa
     8581 caggacgagc aacagcgcgg cgcacataat cgtggcgatc gtaacgcccg agacggggtt
     8641 gctggaactt ccgaccaacc cggccatgta ggccgcgacc gaggagaaga ggaacgccgc
     8701 gagcaccatc acgatcgtca tgaggaggcc gatcaaagga ctgttgacga cgctggtata
     8761 gatccaggcc atcgggatca gtgagagacc aaaaggcacg acgatccagg ggagcgacgc
     8821 gtcctgttcc gtcctggcga tgaccgccgc agatcgagac gttcccgctg gacgataact
     8881 ttcccggagc gtctgcagac ttctccacaa aggctcacgc aacatcgtca aggtccagaa
     8941 tccgccggtc agcatcgcgc cgatgccgac atagcggatc tggccgctcc agatcgttgt
     9001 cgcggccgcc aggccggtga gatcgggcgg cagaccatac aggaacccat aggccggcat
     9061 caggaccatc catcccacga cgccgccaag aaagacgacc accgccgtct gaagcccgat
     9121 gatgaagccg acggctagca gcgcgggcga cagattgaca cccgcataga acacggtgcg
     9181 tccgacctgc gcggcccctt ccagtgattc ggcccacagc cgcacgccgc tttcgccaaa
     9241 cttcaccgcc ccgcccagga gggcggccga caacagcgcc cggaaatcgc tggtcgattc
     9301 ggccggggcg gcatttcgtc gatcaggtgc ggcgaccttc aagacttctg cggtcgccac
     9361 cccttcgggg aacctcagtc gagcggtgac gatgagcgcc cgccgcaacg ggatggtaaa
     9421 gagcacaccg aggagacccc cgactgtggc gatcgaggcg gtctgccagt agtcaaagtc
     9481 ggaccaatag ccgaccagca gcagcgccgg gatggtgaag atcacgcccg cggccagtgc
     9541 ctcgcccgac gaggccgccg tttgaacgat gttgttctcg aggatgttgg accggcggaa
     9601 caagcgaaga atggccatgg acgcgactgc ggctggaatg gatgcggaca cggtcatgcc
     9661 ggcaaacagt cccaggtagg cgttggccgc agccaggatc gccgcgagga tcaccgacag
     9721 cacgaccgct ttgacggtaa tttcagggag tgtgacggtg gcgggaacga cgggctggtc
     9781 gtctttcatg cgccgtgtcg aacagatgat gcaccgtaca tagagacact cgtgcccgtg
     9841 gcagccaggg tgctgacgga ggtggtcgca tcataacgca gcgcacgaaa aaggcaacag
     9901 aggacacatc accgtatcga gtaccgttgt tggccttagc cgtcagttgt caggtttggc
     9961 ctattgcgcg caacgatcgt gtccgttcac tttctttccg ccagcaatcg aatgagcgaa
    10021 tgcgctttgt cgtccagctc cagcaacgcc tcaagaatgc gaaataacag catccccatc
    10081 tcacacaaca ccctccacag gaggttgccg atgcccagga ctgtgagacc tccgaagagc
    10141 agcgcatccc ggttggtgtc cgtgtactcg gaagcaacac ctgtgaggac caaagtgacc
    10201 atgagcacgc cggccagagt gatgagcact gcgccgatga tgtaggccac ttggatagct
    10261 gccggggtga tcagcgtccc gaaggaaaag aatcctccgc gacggtgtct ggtgtcgatc
    10321 gcgagagtct ccgttgaagg aggtgatgaa gcaggtgctg tgtcatccgg gagggtggcg
    10381 cgacagattc tgcaccgacg aaccccgctc gggttctcaa agaaacattc cacgcatcgc
    10441 atacgagacc tcctgaccac cgcagagggt tcaaaaatga taggtcatcc ttgtgcctta
    10501 agaaagggaa aaggtggcag ggaaggaaca cgtgtcggga atctcctctg gctggcttgc
    10561 gcatagcttt gtatcctccc taaaatagat ttccctgcga ggcgttcatg cctgtcaccc
    10621 ttcatatttg tcgcggcttc cctgcgcgct gttccgagga ggtatccatg acttgtcttc
    10681 ggcagttggg cgcaatcgtc tttctgagtg ggatactcgc cgggtgtacg gacggcgtcg
    10741 tgaaaccggt cggtctggat gtggcgctca gaggagtgga agaggatatg aaggccgcct
    10801 ccggagtgag tatgcacgat attctcagcg gggacgcggc gcaagaggag gagttcaaac
    10861 aggcgatccg tcaagagcag tgtttctacc ggagaccaaa cccgcttgtc cctgtcatca
    10921 ataaagagtt taccttgacg ctacaaggaa ccttcacagg acaaggaaag tttctggtgc
    10981 tggggattcc ggttccagca ggaggtgtcg agatcagtgc ggcgaagact cttcaacaaa
    11041 cgcttgccct tccggtcaac tttacgtcag tctcgtcctt gcctgatgtg tatcttcaac
    11101 agaaggccag ttacgtgaaa gacttcccgg agtcggagaa agccatgtac ctcgaagagg
    11161 tgttcaagga acgcgatgtc atacgcgcca gcatcaaaga gctgatcgcg agctattcag
    11221 aggaacgttg tcgccggatg cctccggatc cactctcgaa gaatcatcct gtcgccccaa
    11281 gacctgctca ttaaggccag acgagcgagg caggcgagat gggcgagacg agcgggaggc
    11341 gtaaggcgtg aggcggaagg cggggcaagc gagaggagcg ggaggcgtaa ggagtgagga
    11401 gtaaggggtg aggagtaagg ggtgaggagt tagaagtgag gaggaaagag ctgatggctg
    11461 acagctaatc gcgaagagcg gaaagagcgt gaggtggaag atgcgacgag agcgaaaagc
    11521 gggaggcgtg aggtggcggg gaaggtcgag gccaagcgct ggctcctcgt cgaagcatgt
    11581 aaaccgattt gtttaggcag gagccactca gccagcgatt tatcttctcc ttctgagcct
    11641 atattgatcc atcagaattg tgattattcg tcccctcggg tatagtgcgc cacaccctga
    11701 atgatcacgc tcgacgagat acgtcaacgc cttgtcgcgg gaacgttcga gttcagtcgc
    11761 catgctttca aacgagccgt tgagaggaac atcagcgagc gcgatattcg agaggctggc
    11821 cgtcgagccg ccattattga ggactatccc gacgacaagt atgcgccgag ttgcctgatc
    11881 ttgggattca cggatgccgg gcatcccttg cacattcagg tgtcatacat cgaatcagat
    11941 atgctgaaga tcattacgat ctatcagcct gatcccgaag agtggtatga ttactcccgg
    12001 cggaggtaaa aatgtttcgc tgtcatgtct gcgggaaaac tgaggcacgc caagaactcg
    12061 tcagtgaagt ttttgacatc gatgacaaac ccgtgcgtgt cgaagcaatt ccggctattg
    12121 tctgtgcgaa ttgcggtgag gccgtcttca gccgggaaac gacagaacga gtgcgtcgca
    12181 tggttcacgg ggaagcaaag ccggtgaagt ccgtccagat ggatgtgttc gcctatacga
    12241 agtgaggact ggagtcgttg cctccacatc gggcgccagc gactgttttc gtccattcaa
    12301 tcccccatcc agacagtccg gataatctgc tgactcgctc caaggttcat cactagctac
    12361 gtccgtggtg taggctcctt ccttcgattt tgctacactg ttgcccctgg agaatgtgcc
    12421 cgtgaaagaa caactgaccg cggcgtttca acaagcgccg gaggattata tggtccttgt
    12481 cgaagagctg ccggctagtc atatccagga aacgtgctca tggtgacgcc gatgtcagtc
    12541 ctcgcgccgt tcctcgccgg tctctgtctc gtcgcgctgc cggtgtcgac gcactcggtg
    12601 cagcccgagc cggatcgagg cgtttggcgc acggcggcgc ctgcgccgac gaaacggaca
    12661 gaggtggctg ccgcgacatt gaacggtaag atttacgtcg tgggcgggtt cgagcagccc
    12721 ggcttgggta atctgctgaa tctagcgatt accgcatccg tgcaagaata cgatccttcc
    12781 acggatcgat ggaccactaa agcgtccatg ccggtcggcc tgcatcatgt gggcatcggt
    12841 gtgacgggcg gacgattgta tgtcatcggc gggtacacac agtcgggact caccgtctgg
    12901 caccctgtcg ccactgtgta cgcctacgat ccagctacgg acagctgggc cgagcgggcg
    12961 accatgccga ccgcccgcgg cgcgttgtcg gtgaccgtgc acgaagggaa gctgtatgcc
    13021 atcggcggct acgaccggaa cgccaatagc gcagcggtcg aggtctacga tcccgagcgc
    13081 aacgcctgga cctcgcgcgc accgcttccg actcctcgtg atcatctggc tagcgcgacc
    13141 gcggccggga aggtgtatgc gatcggcggg cgagtgaatg gggactatcg tcggaatctt
    13201 tccgtcatgg aactgtacga tcccgtcacg aaccgctggg cccgcgctgc ggatcttccg
    13261 actgcccgca gtggaatcac cgcggctgag gtcggcggca ggatctacgt cttcggcggc
    13321 gaaggcggcg gcggcacgtt ccgtgagaac gaggcgtatg acccggtgcg cgacacctgg
    13381 cagaccatgg cgccgatgcc aaccgggcgc cacggcctgg ggtcggctgt ggtgcagggg
    13441 cgcattcacg tcctcagtgg cgggccgact cccggaggct cctttagcga tttgaacgaa
    13501 gtctttacac cgccgggaga gagaatgacg aaagaaggat aggacggccg tttgaggcga
    13561 actcctcgaa attcagtcgt tgtcggtgtg gatgtcggcg gagtgaagaa gggctttcat
    13621 gccgttgcac tcagagacgg gcaattcttc gggaaactga acaccctcga cgctgtggca
    13681 gtgatccagt ggtgccggtc tcttcgtgcg tcagccgtcg gtatagatgc tccctgctgt
    13741 tggagccttt caggacatgc gaggccctgc gagagcgcac tggctatgga aggtatccat
    13801 gctttcgcaa cgccgagtcg ggcggtaggg gggcgccatc cattttatgg ctggatgcga
    13861 aagggggatg cgttgtaccg ccgtatcgcg catcattatc cgctgttcga cgggacactc
    13921 ccgacgtcca ggccggtctc tttcgagacg tttcctcatg cggtgggttg tgcgctggcg
    13981 ggaacgatcc tctctggcaa gcgaaagcgc atcgatcgcc tcaaattgct tcgcgagacg
    14041 ggactctcca cagagtcgct caccaacatc gaccagatag atgccgccct gtgtgccgtg
    14101 accgcccggt atctcctggc ggggaacgtc aatatgtacg gcaacttaaa ggaaggcttt
    14161 atcgtcgtac cgaagataga aaggtcctca cgaaagcccg cttccatttt ttgagggcat
    14221 caaggggaag cctcgggcac aagagccaat tgtttgagcc atgagctttc ttgctcgctc
    14281 tgtgaattgt caaagagtca ttcgtgtgag taggccgccc attccaccaa tcgatcctta
    14341 cgaaagacca ggctccattc ataataccgg cctcgttctc tctccga
//
LOCUS       DATIFR010000024        31669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1353709, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000024 DATIFR010000000
VERSION     DATIFR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31669
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353709"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="yajC"
                     /locus_tag="VLD60_01170"
     CDS             complement(<1..334)
                     /gene="yajC"
                     /locus_tag="VLD60_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSB43588.1"
                     /translation="MVMESVAWAQGIGGSGSGSGAGTLLSLVPFVLIFVIFYFLLILP
                     QQKKQKQQKALLDALKKGDKVITSSGIWGTVTNLGKETVTLQIADNTKIKMQREFISR
                     LRADEEDKD"
     gene            complement(356..1528)
                     /gene="tgt"
                     /locus_tag="VLD60_01175"
     CDS             complement(356..1528)
                     /gene="tgt"
                     /locus_tag="VLD60_01175"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSB43589.1"
                     /translation="MNFSVGRQDRSSSARTGTLSTAHGLTETPAFMPVGTLGPVKGIE
                     PQDLKQLGFNLMLNNAYHLYLRPGHKVIAEMGGLHRFTGWPGAILTDSGGFQIFSLAK
                     LCKITDDGVSFQSHLDGSTHFISPETAIEIEEALGADIIMAFDQCVALPAERDVVRDA
                     VRRTTLWAKRCQASRRRTDQALFGIVQGGLDRELRAASARDLVELGFDGYAIGGLSVG
                     ESTDEMYAMLDVTVPELPASKPRYLMGVGMPENLIEGVARGIDLFDCVVPSRHGRTGW
                     LFTSFGRVVIKQARYLRDEQPIDPHCGCPVCARYSRAYLHHLYNAKEMLASRLNTIHN
                     LWYFADFMRRMRTAIGQGSFSEFREAFYRAQKREAPEVAALANREAGTIRRTALDV"
     gene            complement(1555..3309)
                     /gene="argS"
                     /locus_tag="VLD60_01180"
     CDS             complement(1555..3309)
                     /gene="argS"
                     /locus_tag="VLD60_01180"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSB43590.1"
                     /translation="MSQGVVQDKVTTALLDALNGAKEKGQLKTEAWPTLSLDAPKRPE
                     WGDLASTVAMSLASSEKRTPHDIAQIIIDNLPQREQLFDRVEIVRPGFLNLTLKPSLW
                     QEVLKEIATQGADYGKADIGRKRRVLVEYVSANPTGPLHVGHGRGAAVGQAVARLLEA
                     VGYNVVSEYYINDAGRQMKFLGASVYARYQELCGQIVEFPADGYHGTYITAVAERVKQ
                     QLGDALAGHSPAEIEERCRALAYQELLGLIQEDLKSFGIEFQSWFSEASLLTSHAVER
                     VLGELTSRQLLFEQEGAWWFRSSVFGDEKDRVVKKQDGEYTYLASDIAYHWDKLQRGY
                     DVLVDVWGADHHGYIPRMQAVMQAYGHPKERLQVILVQLVKLLRAGVEVKMSKRTGEF
                     ITMREVIDEVGADAAKFYFLMRDANTHLEFDLELAKQRSADNPVYYVQYAHARIASLW
                     RVAASRGIACLPPSQTDLTVLTDPDELGLIRKLSAYPSVLQASAAGYEPHRITYYLQQ
                     LAALLHTFYNKHRILPPAADKEVFEQVLPGIATGEEPERETLSPERTAARLALMSSVQ
                     QVIKNGLGVLGISAPDHM"
     gene            complement(3445..4398)
                     /locus_tag="VLD60_01185"
     CDS             complement(3445..4398)
                     /locus_tag="VLD60_01185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB43591.1"
                     /translation="MRRSILIIHPGALGDILLAVPAMRKLRAQFPQHELVLCSNESAA
                     RLLLEYRVIDAWLSVQGRACAELFDPEASIGGELQEWLERCDCAVAWMQDRHGSLART
                     LEKAGARKAIVCSPFSMTLKASHQSDRFCETVQELLIHDAELHRLQLPDAVSLRGRSC
                     MEESGVGMDCNFTAFHPGSGSPRKSIAIATMATMMARLRGEGMLPIVIEGPADHEAVG
                     CLLQKLPFPAVVLRDLELRTLAAVLSLAKNFVGHDSGVTHLAGLLGVPTIAFFGPTDP
                     DRWAPLGSHVTILKAPHASESLAMPFSDVSATRHSIGDPRD"
     gene            complement(4406..5026)
                     /locus_tag="VLD60_01190"
     CDS             complement(4406..5026)
                     /locus_tag="VLD60_01190"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HSB43592.1"
                     /translation="MITDVTGILLAGGKSRRMGGDKRFLHVGEHTLFDRSLAVLRSIF
                     RDVLIVIAQDSPPLETDAAVIRDLVPDCGSLGGLYTGLSQATTEHVFVVACDMPFLNQ
                     EAVRYVIGLKEEADIVMAEGENGLQPMHAVYSRRCLPVLEEMVRTHHLKIQDIVAHPS
                     LKVRLINVAELSRIDPEGRSFLNVNTPADLEAARRLHRPPRDPHAH"
     gene            complement(5121..6896)
                     /locus_tag="VLD60_01195"
     CDS             complement(5121..6896)
                     /locus_tag="VLD60_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB43593.1"
                     /translation="MKNSLSRKARVVIATAFGVILASGSTGVSLFAQDAVPEGFKKGE
                     LAPEPSAEMIEAGKRVYFTKCVWCHGVDGAGDGPGADRLWPRPRNFNQGTFKIRHTAS
                     GELPLFDAKKPVPGQNDLFETVTHGLPGSAMPSWEGILTDEQRLQVLSFVTTQLVKDR
                     KFTDKQSEAQTILQLGDLKPIAATEESLKKGSELIVEKKCVECHGAEGRGDGNAFNLK
                     DDWGFAIQPADWHKCWNFRGSRQDPYNVKNIFRTFSTGVNGTPMPSFADNTTIEERWH
                     IANWVNFLCERDTDGKPLPIDPLTDKPKINFVVPSSPVEGEIPADMENEAWQKTPKRY
                     VAMGGQITHKPRNFVNRIDDIWVRSLYNDKEVVYLLEWDDRTKSVAEGKLPWAPTQVN
                     VDVKEQDPKTGEEGSIAANQNKYAVYNDAIAIETPVKWKELPAPIKPRYLFGTNEQFP
                     VDIVKWEADGSIRAFKGTGWDKDFEERDTYEESLKVLKSEWKNGRWYVMIQRPLGNKK
                     DQDYDEDTFFEVGQYIPTVFFAWDGHNGDAGRKMAVSAFYYTFMQPPTPKETYIYPVV
                     IAFGVVLLEGWVLTRRANKKKGKTL"
     gene            complement(6946..7983)
                     /locus_tag="VLD60_01200"
     CDS             complement(6946..7983)
                     /locus_tag="VLD60_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB43594.1"
                     /translation="MKALMSVGALVGVVGLLLLVGMIVDVIPSNTVRLVEGYMPVQML
                     LEFALFVAGFTGLSYMASSMGMAIPRFWQGIGFWAFVLLYLKFRVYPPIPFSVRAMYG
                     TVSLVAVFMWVSANEEDWKKFKQPIMNVLDAQSGANKLLRYGYLILIPVLVGWTSFNA
                     MVPKSEEPIELRTVHPAPPASTKVAGKTYVLQTSQNPYRVNPEGKYDQEFTNANIVEQ
                     GMGRLMKPNANPWDDKNQGYLKYVREGGEIFFQNCHFCHGDNLNGRGLHAFAFNPIPA
                     NFTDPGTIAQLQETFIFWRVAKGGIGLPNEGFPWASVMPPWEQHLTVDEIWKVILFEY
                     WHTGYYPRTWD"
     gene            complement(8026..9336)
                     /locus_tag="VLD60_01205"
     CDS             complement(8026..9336)
                     /locus_tag="VLD60_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB43595.1"
                     /translation="MSNLINEALSMGWVVLALLAGLLIYFQVSISDPAAKKRATFKTF
                     IGIIAAFLLFIAIANYKNNFYGENRLLPVSLVMITATSFVLALYFTNLGALLKIGGFM
                     FFVAAFLSGYGNWLPQVEGGFPPKEEKLDFSSMTPQQLADEGEKIIFGGIGQNKVQGA
                     IGKGQCPLCHAFHAGMLGERAPNLLGLPERAGKERLEDPKYSKGNPSKRDYAEKEAFP
                     GAGTAETGQEYIAESHSCPSCYVVAGYGVKGTNDKESPMPKIHKPPISLSLPELAAVD
                     TWLYVREGREAPSFEEIVKSYEKFIPEADRPKQQEDKPAGATALLADGSEPVDQIFAK
                     AQCVVCHTIPGIPGATGTQGPKLEEGTNAPQRIKDPAYKGTAHSTPEYIMESVVSPST
                     YVVKGFPDNLMPKVFGQKLSAGALKKIVDYLSQVKAGAPPPKIS"
     gene            complement(9418..11334)
                     /locus_tag="VLD60_01210"
     CDS             complement(9418..11334)
                     /locus_tag="VLD60_01210"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSB43596.1"
                     /translation="MGLIARKKLFSIMALCAMVGLLLLPIVMSLPTLAAGQETPAVDG
                     AKKDGDKVEKGRDVYYKTEGIVVGAPAPKTTDGPKDYPRYNFESRVLLWFANQQHLYY
                     GSFVLAVPIFCMVIEFMGVVTKDKALAKRYDQLAYDFIKISLTAYSLTAILGGILIFT
                     FLTLYPAFFGYLSSIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMSV
                     ILNVIGTLLMFLANSWIGFMMSPAGVDEQGRFLGNIWHVIHTALWNPLNVHRILGNMA
                     FGGGVVAAYAAYKFLAAKTEEDRAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYAY
                     RQQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQAMGRMRGAEKYQRYIKYLVFL
                     LVCGFLVFITPHTMVMSPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFVW
                     YMRGNKVSTVSWAKFGNIFMGVFFACAYVNIIWLAIYGYYIPANVRVGLSVPQVATTL
                     SCLFFMFALNSVMMKGAKQMGPIEWGKISARSQYALIMLATAFTWMMGLMGYIRSSVR
                     LFWHVNEIMRDNSPWAYTHTVGFAANMISFNVLFFWVSILFVFWLGSLTAKKVPVGEK
                     AGVPGSAPQPAGSH"
     gene            complement(11409..11531)
                     /locus_tag="VLD60_01215"
     CDS             complement(11409..11531)
                     /locus_tag="VLD60_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43597.1"
                     /translation="MGQSIVYGVISLIICVAYFTLVDHFLMDMQGLDYWYLFRK"
     gene            complement(11588..11713)
                     /locus_tag="VLD60_01220"
     CDS             complement(11588..11713)
                     /locus_tag="VLD60_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43598.1"
                     /translation="MATATAPDSELKVKVGKMIFYITAAVGLWFFYWFAGIQCPC"
     gene            complement(11943..12842)
                     /locus_tag="VLD60_01225"
     CDS             complement(11943..12842)
                     /locus_tag="VLD60_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB43599.1"
                     /translation="MLETKFKVAFLLTVLAFAAIPIIGILRGTTLTPFEAGPPGAIET
                     ARSEPEAAPEEEPIREEMVTIPAGPFLRGTTSGGFDEQPQRTIFLETFSIDRYEVTNH
                     QYQEFVVATGHRKAGPPSRYAKSIGKMRGTNQPAVYVSWDDASEYCRWKGKRLPTEAE
                     WEKAMRGSDGRLWPWGNAEQPNGANWARVQDGHEVSARVGSFHADKSPYGVMDGAGNA
                     MEWVADWYDEGYYKSSPEENPPSPEYGTYRVLRGGSYTTTGADIRITSRSKMVPDFRD
                     ETIGFRCAILGISEGQKRQEKSG"
     gene            complement(12849..13766)
                     /locus_tag="VLD60_01230"
     CDS             complement(12849..13766)
                     /locus_tag="VLD60_01230"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB43600.1"
                     /translation="MENKGVLIGSIIFVFGSFILMIGLMLWESNKAYKMKELAQSIKT
                     ESRPTVSSAPAQDYSMYKTKMGDEGREMVQIPEGPFTMGSQDGDPDEVPEHQVFLKGF
                     YLDRKEVTQDEYMRFAKMTKRPMPRIEVFEDDQSKLLQPEFAAMSVSWDEAVAYCKWA
                     GKRLPTEAEWEKAGRGEGKRKYPWGERFAAHAANVAGSDDGYKYLAPPGSFEAGRSPY
                     GIFDMTGNVAEWVADTYDESYYKKSPYRDPKGPTAGDLKVVRGGSWRETEHNARLSKR
                     FAAKHWRTDITIGIRCASDLDGTNSDSSS"
     gene            complement(13794..14171)
                     /locus_tag="VLD60_01235"
     CDS             complement(13794..14171)
                     /locus_tag="VLD60_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43601.1"
                     /translation="MDTTQASSMPLQKVFDMVFGLAVMGTVGTLIGYIMGGGLVPVAI
                     GVGLALGGVIGFLGGRRFLVSILVGTVLGGALAWLVAGDERIWVGAGAGAAMGGFLGV
                     QISMLLDLRAARKTSPEQSGPSV"
     gene            complement(14185..14841)
                     /locus_tag="VLD60_01240"
     CDS             complement(14185..14841)
                     /locus_tag="VLD60_01240"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSB43602.1"
                     /translation="MKSRLVLCGLAAMSWLAGSIAVMEGSARAQGRTLVLEDFQAKEA
                     DGFPSNWDHENQRSQSKGREAYKVQTEGGANFLSAKDAGQRIKKKKIDWDPKAYPVLT
                     WRWRLNKAATGTEPVAAIYASLDTDLLFIPVFTKYIWSSTKPEGTLTEGGMFSGSEIV
                     VQYGTKDVGQWFEEKVNVYEDFKRIHQHEPAAKAWGISIIAGPGVEIDFGPMIAMSGK
                     "
     gene            complement(14867..16720)
                     /locus_tag="VLD60_01245"
     CDS             complement(14867..16720)
                     /locus_tag="VLD60_01245"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSB43603.1"
                     /translation="MLRYFTLSVPLALLAALFVLGPGAQHPAFAEDAADLYFKTQGIP
                     QGPAAPSPTETVYPRIGSFDSRLLVWFVTQQHTYFGGFVLALPLFCALLEFLGLITKK
                     PALSLRYDGLARDLAKVALLALSVTAVVGSLMLAMFIFLYPSFMKYMGGTFKAFMPAY
                     AIIFVGESLLLIVYYYSWNRMAEPGLKWIHAAIGILTNIFGTTLLLLANAWAAFMMSP
                     AGVDAQGRFLGNGWHLLHSALWNPLNVHRFLADIMSGGAVVLAYACYRFFTSKTEGER
                     AYFDWVGYIFLFVTVCALLPMPIAGYWLMRSVYAFRQSMGVTMMGGLLTWLFVVQALL
                     IGALFLGVNYYIWQSMARIKGGERYQPYYQLLLGALTLALFIWLTPHTVMMSAGEVKA
                     MGGAQHPVVGNYGVMSAKNGAINVMILLTTLSFLYYRRANRTMTVSWVRTGNIVIGGL
                     FAVGILNVIWLSIYGFYLPAKVRVGLSSPQAFTTLTVIFASVLINRFMLKGATLHGSV
                     QWGKIPVRGMVGLFGLAAAFTWVMGLMGYIRSSGRLSWHVNELMADVSPWAFTPDLEF
                     AAKMVTLNMVVFWGAVFALFWMCQRGQQPAMGEELVEEKSPALSPAPSQEV"
     gene            complement(16743..17786)
                     /locus_tag="VLD60_01250"
     CDS             complement(16743..17786)
                     /locus_tag="VLD60_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43604.1"
                     /translation="MALRVLPSLSLAVTEDSVRKQKRIVMFIPGLVAFGIYRLVKHML
                     PLTEPVVLLLVSGIVGLITALLAYRVGRRTAWRVISHQDGIKLLTWMAGWIGFVYGIQ
                     LSLLVLALLWLVGYDYLQHPDGPAMMAIIISCTAVARDAFEIGLVRKIGLMGRPFPTF
                     PNGVALRGLIGSRTAEVGPWIVGGLIVGATGALLGHITENSQVAVLIQLSAVTLLGGA
                     IALCAYFGGLRPNESWTAGLLKTKPSELLKYWWWPGLAFSSTYYLVAAGIVIFLLRQP
                     GIPTAYAVVGGGLVAGLMALYGYYLGYRRQVEEQQGQGLSNALLRCPFVMGILGKSTN
                     MGAGVTAAPASKV"
     gene            complement(17870..18337)
                     /locus_tag="VLD60_01255"
     CDS             complement(17870..18337)
                     /locus_tag="VLD60_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSB43605.1"
                     /translation="MHTEGIEERAYEDGALQWFIRSECRGCGFKVGVDVPAGQVNGFV
                     DRVMWTDDALHRLDRMPPYLAPLFREEVEQDVRARGERVITYDTLLRPRTGERIEWEP
                     EAERRLDNVPAPVRAMARIELERTAADRGQTRITVALMEEIKAKYFGMAAQKT"
     gene            complement(18482..19195)
                     /locus_tag="VLD60_01260"
     CDS             complement(18482..19195)
                     /locus_tag="VLD60_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HSB43606.1"
                     /translation="MDWLKNPGFFGTHATIGADISQLMATFFTCLFVIGWIQARKRQS
                     DAHHWLMLGGMVAMLGFFVSYYLFRQLGVLAFEGKEGFGGSDALYHNVFIPLLTFHII
                     LVIIGLVMAVYMIVLGFRAQAVEQGRRILSGRILQTTWGKIGMIFGVLCALVAVYLLY
                     LVAADRFRMGRLVVWVGLLALIAIVFAVEMAIQRIWPDGGQRHRTLGRFTMIIYCVLF
                     VTGTTTYTMLYILYPGKIG"
     gene            complement(19204..20229)
                     /locus_tag="VLD60_01265"
     CDS             complement(19204..20229)
                     /locus_tag="VLD60_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB43607.1"
                     /translation="MPTFKDISLEGMGVSRELSLAEIFQLGYYWETKILLTAVRLDVF
                     SVLDGKPRTAREIAGKIGAHEPTLTLLLNALVAMRLLAKEGETYRNSVAAEKHLVRHS
                     PQYIGHLLLLHDAEWNNWGKLEETIRSGHRSVDQHVFETDPEIGSNVLAVLHRIGQQS
                     GPDFAKRLQLAGRVRMLDLGGGAGTNAIAFCHIYPELTATVFDLPATLRLTEKTVKDA
                     GLESRISLLPGDFNKDPLGGPYDVVLMSDILHYQTFETNEALVKKVFTQVSPGGRLII
                     KDRFLDEAGTGPAWTTAFAVHILVNTQQGGCYKTAEAMQWMTKAGFVSVIELERTAVV
                     QGMRPTR"
     gene            complement(20358..21152)
                     /locus_tag="VLD60_01270"
     CDS             complement(20358..21152)
                     /locus_tag="VLD60_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43608.1"
                     /translation="MKSALSVVFGVAAVAFVAAPLTSFAGGTIAGKVTYAGKSEQKEF
                     SFAKFPNPKFCPKIPDKTLMDGDKRFLKTIEVGKDGGLKGAVVAVSDIEDKPFMDGYA
                     GTEVTAAFCQFLPFSGVVVNNKSFKVENTDADPDDPKSVQGVLHNPHSFVVKGASSAT
                     GFNIGLAKKGDKLDKPVVFRGGAEKDGFYRLQCDQHEFMQSFFLPVSNAHFAVVKDDG
                     SFELKDVPAGKHKVVAWHPFAAKGKKIEFEVEVPEGGTANLKAEIK"
     gene            complement(21372..22733)
                     /locus_tag="VLD60_01275"
     CDS             complement(21372..22733)
                     /locus_tag="VLD60_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB43609.1"
                     /translation="MRNVSVGLGVSFVIGLFWLFVATGPAFAGGTATVPKPIQNLYDK
                     NEFQQTLDEIGKLDKERAASPEVQRLKIRSLLKLGKPKEALEEYDALEAMLRQEDQSV
                     LREVALGFIVVMMKDMREQMRGAAYTALKEIDSDDIVPYFEDGLSDGSGPVRVLAVEG
                     LGRTEAGRKSKKLRAALDDQAGLVKARVVKALGKSGDPSVMPLIEAATKDELTPVRIA
                     AYGALIRLGRKDAWEQLRQGVEAPNPEDRAEAIRAIADLKDQRGASVMMELLTYQQPS
                     VRAAAARGLGHLEKQEARGSIEQLLKDPVPAVRESAAASLADLGGKESLPALNQALKD
                     GVVTVRAAVVAALLQLGQPFETVAPTVRALAQQNDTAARSSAAFALGKATGVNVPGAV
                     DMLSNLAADPLPGPKIVAIRSLGHIGDRQVVPFLKSSLHDQNEAVRSTAAGALLHLMQ
                     RKK"
     gene            complement(22741..23379)
                     /locus_tag="VLD60_01280"
     CDS             complement(22741..23379)
                     /locus_tag="VLD60_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43610.1"
                     /translation="MRNGFARSTRVLVFLGLALSLSIWSPARRAMAGDTLPIQPEFSG
                     TIIKQVNGKKYHAQVFAKGDRLRLEYKYAIRTEHGYAAIEIIRLDQSETWYLLAQQKE
                     LLVVPLDPDDVLPMRAGLPGERDRMLVGDATAAGRTAQLYEVQTDRHGRAERFYEWVD
                     KEVGIVLKLVSRDRDWSFEYERIRLSPQPAYYFDEPPGYKKRIGAAGPRQQG"
     gene            complement(23386..24312)
                     /locus_tag="VLD60_01285"
     CDS             complement(23386..24312)
                     /locus_tag="VLD60_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB43611.1"
                     /translation="MNDKLMTGLLGALSTILVVLTVGPAPAYDVIDVQHGGMIEGTVL
                     LDGAVPEPKGFNLITFPDPAYCGRISNGRGWRLLHDFVVDAQGGLKDAIVLLEGVEAG
                     KPFEMSVPLIEARDCMFQPFMTIVRNGHAVEVINMDPVMHDIQGYETSIEAGARTLFN
                     TPLVMNHQHRRGDLHAIHNHAPGKSLVGPIYLNKGRRTFYMQCGFHAYMESWAMAVNN
                     PYYALTDANGAYKIENIPPGTYQLVVWHPQTGPGVTKMVTIQPDGKLVEQLSLNPPKG
                     NRSAFKVMDNPRFGPDTLGYPVDIQPLVEHQH"
     gene            complement(24332..25243)
                     /locus_tag="VLD60_01290"
     CDS             complement(24332..25243)
                     /locus_tag="VLD60_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB43612.1"
                     /translation="MNSALAIGLCSWLAVTSSVAWAYEEIRVTDGGTISGKVTITAGK
                     PVPKGFNLITFPDPVYCGRISTGTGWRILKEFEVASNGGLKDAVVWLSDVTKGKPFKF
                     EPQTIEARDCRFLPFVTVVQDKADVTIMNMDPVMHDIQAYETSHLGPRVLFNTPLPMN
                     PHHKRSVGAESHEHLAGQPVTETIHMTKGRKFFVMQCGFHAYMESWGLAIENPYYQVT
                     GSDGTFSLSDVPPGDYLLTAWHPGVGTVLEKKITVPAKGAVQTDFLFESPKGRRSAHE
                     IEENPHFGLQALGKTVDIRPTLELQVP"
     gene            complement(25386..30227)
                     /locus_tag="VLD60_01295"
     CDS             complement(25386..30227)
                     /locus_tag="VLD60_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSB43613.1"
                     /translation="MFSHTQRVCVHTRMLGMMAAVALLWAPLASAENSSSHVAMNHQL
                     PAWAEQLKGQTIVEDSLSGKANRSAMVEQQHQRIMDHMSNDPQVQGVNTGMYNTQSMM
                     HQYGAGGQDMLLVSDPRVEPVAMTSGGKCPATAPVKQYNVSAINVEITLNQWLDFYPG
                     YMYVLDENIDKVRAEEAKNKEGREKEGHDPGAVIPGVQAQWIQPLTIRANQGDCVKIK
                     LSNKLEGGEDVSLHIHGSAMVVSATGASAATTNPDSIVVKDKSGEFEWYIHPGTQEGV
                     RQFHTFSNDRELTVMGLFGSFVVEPRGSSYWEPLGSGEATPATSGWQVMIKNGTGPDF
                     REFVLYYHEVGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRAINYRSEPFGINNMHVQH
                     EYFGFEDESMGYSSYTFGDPSPTIPRSYIGDPAKFRLVHGGSEVFHSHHPHGGTIRWP
                     RSPRAIDDMNLWTTSGNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGSGLCQQLA
                     GDFLFHCHVAHHYVAGMWGYWRVYNTIQQGDLRNDVMPDLRELPDRKGRIKTPISSDK
                     LIGQTVDWFGKAFKIVEKGKSDWKSNPATITIKDWVEMQLPTMGKPGHKDDEKGQIIS
                     YDATVLDWTWNGNVAMSEKESTTDNPKYKSSHPGKRHPIMFEPLTGKVAWPHLTPHFG
                     KRVMFSPNHVGAPWLEMIRRDADGQESVDQSLPGENGNWSLCPENAGRKHYNVHFVKM
                     PIKIAKAQGKEPPVVDPNGLIYVLHEEEAAIRANDDLKYPLVVRGNIYDCLDWTLTSE
                     WDDDDYTNFQSSKINTHWHFLQFDNQASDGVITGFSYEQSVRPFTMLEKKTKKGLPAP
                     MNTVLTAAAKKGATSITVKNAGQYHVGTLILVGADNVKGNEVSRIKAIKGNAITLAKG
                     LKNDHPANDIVTVEFVRQRFWVDADVGTVFWHDHAFGATTWPHGGFGTFIAEPVGSTY
                     HDPKTGKAIRSGPIADIHTNEPVGHGVNNSFRELMVQVHDTVPHTVNIVTAGNPPGQP
                     VEVALEAGKTVSFMMPEKLYMTPMPFLNGGTHTTGSGLNFRAGPIAQRLATNPDVSQA
                     FNSQIHGDPYTPTLRAYVGDTMVFRLLHTLMNESMVWTISGHTFWSERYASDANRKNS
                     IHIGIAERYDLVVPEAGGSRHQAGDYIHFNGRSSKFSEGGWGIIRVYDKDQADLKKLP
                     AGFSSKGEMPKALPVCPADAPVKSFNVVALDYPGMKFNAKAPESIEVDFERKIQITNP
                     DAKIYALEEDAAKVSGGAQPMPLTLRVNVGDCVKVNLKNKMKDSKASFSAIGLAFDPK
                     DSLGANVGNNAGDQTIAPGGERTYTYYADPFNGETTSLVWDWGNVMTNPRNGLFGAVV
                     VGPKGSKYRDPKTGADLSNKNAWAADVIIDRSVPGNETRPNYRDVALFFQDEDNIIGT
                     SFMPYVQNVAGLTGVNYRSEPYKYREEQGCSLGKVFQPCKADKPEDPATPIIESHAGD
                     PVRIHVIGASNEQNGMFSVEKHEWPIEPFMRGADQISVVEYSGSETLDAFIPSAGGPY
                     RLPGDYVYSNQRLPYSQSGQWGYLRVLPAGDQRLLPLAGAGAGMKSASLEEAAHAIPV
                     AAK"
     gene            30543..>31669
                     /locus_tag="VLD60_01300"
     CDS             30543..>31669
                     /locus_tag="VLD60_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSB43614.1"
                     /translation="MLLKRWLVGDPLKTAQASHERLSKTLALAVFSSNAISSVAYATE
                     EILLVLILAGTAAIAWSIPVSLAILFLVAVLTVSYRQIIYEYPEGGGAYIVARSNLGD
                     SPALIAAGALMIDYVLTVAVSVAAGVAALTSALPSLFPHREALGLIAILFIVVMNLRG
                     VRESGKFFSIPTYFAIGALGLMVVSGTIQSLLNPSVAPSMPTPSGTSQTVEGLTLFLI
                     LRSFAAGCSAVTGMEVISNGVKAFRPPESHNAAITMVWMSVILAVLFIGISWMAYHYG
                     ILAKEDETVVSQLARLTFGTGTIYYAIQIGTMMLLVLAANSAFAGFPHLSSILARDGF
                     MPHQMATFGDRLVFSNGVIILGFFACLLLVIFHGNTHALIPL"
BASE COUNT         6900 a         9659 c         8226 g         6884 t
ORIGIN      
        1 agtccttgtc ttcctcatcc gcacgcagcc gcgagataaa ctcccgctgc attttgatct
       61 tcgtattgtc ggcgatctga agcgtgaccg tctctttccc taaattggtg accgttcccc
      121 agattccgga ggacgtgatc actttatcgc ccttcttcaa tgcgtctaat aacgcctttt
      181 gttgtttctg cttcttttgc tgagggagaa tcagcagaaa atagaagatg acgaaaatca
      241 gcacaaacgg cacgagtgac agcagcgttc ccgcacccga ccccgatccg cttccaccaa
      301 ttccctgagc ccacgcgaca gattccatca ccatacaaca ggtcccctct ctcgattaaa
      361 cgtccagtgc agttcgccgt attgtcccgg cctcacgatt cgccaacgcg gcaacctccg
      421 gcgcttcccg cttttgggca cgataaaacg cctctcgaaa ttcgctgaac gagccttgcc
      481 cgatcgccgt ccgcatccgg cgcataaaat ccgcaaaata ccagagattg tgaatcgtat
      541 tgagccgcga ggcgagcatc tctttcgcgt tgtacaaatg atgcaagtag gcacgggagt
      601 accgggcgca gaccgggcag ccgcagtgcg gatcgatggg ctgttcatcg cgcagatacc
      661 gtgcttgctt gatgaccaca cgtccgaaac tggtaaacaa ccatccggtt ctcccgtgac
      721 gcgacggcac cacgcagtca aagagatcga tcccgcgcgc gacgccttcg atcaggttct
      781 ccggcattcc cacccccatc aggtaccgag gcttcgatgc aggcaactca ggcacggtga
      841 cgtccaacat cgcgtacatc tcgtccgtgc tttcaccgac cgagaggcca ccgattgcat
      901 acccgtcgaa ccccagctcg accagatcgc gggccgaggc ggcccgaagc tcgcgatcca
      961 atccgccctg tacaattcca aagagcgcct gatccgtccg ccgtctgctg gcctggcaac
     1021 gcttggccca gagggtggtc cgccgaaccg catcgcgaac gacgtcccgc tctgccggca
     1081 atgccacgca ctggtcgaat gccatgatga tgtctgcgcc caatgcttcc tcgatctcga
     1141 tcgccgtttc cggggaaatg aaatgcgtcg agccgtcgag atgtgattgg aaactcactc
     1201 cgtcgtccgt gatcttgcac agcttggcca aactaaagat ctgaaatccc ccgctatcgg
     1261 tgaggatggc tcccggccaa ccggtgaagc gatgaagccc acccatttcc gcaattactt
     1321 tatgacccgg ccgcaaataa agatgatagg cattgttgag catcagattg aagccaagct
     1381 gcttcagatc ttgaggctcg atccccttga ctggcccaag cgtccccacc ggcatgaagg
     1441 ccggcgtctc ggtcaggcca tgggccgtgc tgagcgtgcc ggtccttgcg ctcgaggaac
     1501 gatcttgccg tcccactgaa aaattcacat cccctcacgc atcgcgcact cgacttacat
     1561 gtgatccggc gccgaaattc ccagaacgcc gagcccgttc ttgatcacct gctgcacgct
     1621 gctcatcaat gccaaccgag cggctgtccg ctccgggctg agtgtttccc gctcaggctc
     1681 ctcaccggtc gcgattcccg gcagcacctg ttcaaacacc tctttatccg ccgccggggg
     1741 cagaatgcgg tgcttgttgt agaacgtatg gagtaacgcg gccaactgct gcagatagta
     1801 ggtgatccga tgcggttcgt agccggcggc gctcgcttgc agaaccgacg ggtaggcaga
     1861 cagttttctg atcagtccca actcgtccgg atcggtaagc accgtcaaat ccgtctggct
     1921 cggtggcagg caggcgatcc ctcgcgaagc ggccacccgc cacaaactgg cgatccgagc
     1981 atgggcatat tggacgtaat agacaggatt gtccgcggaa cgttgcttcg ccaattccaa
     2041 atcaaattcc aaatgcgtgt tggcatcgcg catcaagaaa tagaacttcg ccgcatcggc
     2101 gccgacttca tcgatgactt ccctcatcgt gatgaattct ccggtccgct tcgacatttt
     2161 gacttccacg ccggcccgca agagcttgac cagttgaacc aaaatgacct gtaaccgctc
     2221 tttcggatgg ccatacgcct gcatcacggc ttgcatgcgg gggatatacc catggtggtc
     2281 agctccccac acatcgacga gcacgtcata gccccgctgg agtttgtccc aatggtaggc
     2341 gatatcggaa gccaggtagg tgtactcgcc gtcctgcttc ttcaccaccc gatctttctc
     2401 atcgccgaat accgacgagc ggaaccacca ggcaccctcc tgctcaaaca ggagctggcg
     2461 ggaggtcaat tcacccagca cgcgttcaac ggcgtgcgac gtcaagagcg aggcctcgct
     2521 gaaccacgat tgaaactcga ttccaaagga tttgaggtct tcctgaatga gtcccagcag
     2581 ctcctgatag gccagtgctc ggcaacgttc ttcgatttcg gcgggagagt gtcctgcgag
     2641 cgcgtcgccc agctgctgct tcacacgttc cgccacggcg gtaatgtagg tgccgtgata
     2701 gccgtccgca ggaaattcga caatctgacc gcagagttcc tgataacggg catagacgga
     2761 tgcccctaaa aacttcatct ggcgaccggc gtcattgatg taatactcac tgacgacgtt
     2821 gtagccgacg gcctccagca acctggcgac ggcctgtccc accgcagcac ctcgaccatg
     2881 gccgacgtgg agcgggccgg ttggattggc actcacatac tccaccagaa cccgtcgctt
     2941 ccggccgata tccgctttac cgtagtctgc accctgggtc gcgatttctt tgagcacttc
     3001 ctgccacagg gagggcttga gagtcagatt gagaaatccc ggcctgacga tttcaacccg
     3061 gtcgaagagt tgctcccgtt gtggcagatt gtcgatgata atctgggcga tgtcgtgcgg
     3121 tgtccgtttc tctgaggagg caagcgacat cgccaccgtc gatgcgaggt caccccattc
     3181 cggccgtttg ggagcatcga gactgagagt cggccaggcc tccgttttca actgaccctt
     3241 ttctttcgcc ccattgaggg cgtcaagcag agcagtggtc accttatctt gcacgacgcc
     3301 ttgagacacg aaacagctcc taagtcctta ccattgaaag gaaaaattaa cttgccactc
     3361 tagcatacgg ggtaggagga gacaaggcgg ggccgcaaga atgcgggggt ttttcgcagt
     3421 tttccgtggt tcggctccat gtcgttaatc cctcggatcg ccgattgagt gccttgttgc
     3481 agacacgtca gagaatggca tcgctaaaga ttcagaggca tgaggagcct tcaagatggt
     3541 cacatggctt cctagcggag cccaccggtc cggatcggtc gggccaaaga atgcaatagt
     3601 agggaccccg agtaagccgg ctaggtgtgt gacaccggaa tcatgaccga cgaaattttt
     3661 cgcgagagat aagacggcgg ccagtgtccg aagctcaaga tcccgcagga caaccgcagg
     3721 gaaaggcagc ttttgaagca ggcaccctac agcctcatga tcagccggcc cctcgatgac
     3781 aatcggcagc atcccctcgc ctcgaagcct cgccatcatc gttgccatcg tcgcgatcgc
     3841 aatggacttc cggggactgc cgcttccagg atgaaacgca gtgaaattgc agtccatccc
     3901 cacgccagat tcttccatgc atgagcgccc ccgtaaggag actgcgtcag gaagctgcaa
     3961 ccgatgcaac tctgcatcat gaattagcaa ctcttggact gtttcacaaa accggtcgct
     4021 ttggtgggag gccttcaagg tcatcgagaa gggagagcag acgattgctt tcctggcgcc
     4081 ggctttttcc agggtccttg caagacttcc atgccggtcc tgcatccatg ccaccgcgca
     4141 atcgcaacgt tctaaccatt cctgtagctc gcctccaatg gacgcttccg gatcaaacaa
     4201 ttcggcgcag gctcgccctt gcacggatag ccaggcatcg atcacacggt attctagaag
     4261 aagccgagca gcagactcgt tggagcaaag aacaagctcg tgttgcggga attgcgcccg
     4321 gagctttctc atggcaggga cggcaagcaa gatgtccccg agcgcccccg gatgaatgat
     4381 gaggattgag cgtcgcatat cgcgatcaat gagcgtgagg atctcgtgga ggcctgtgca
     4441 gtcttcgagc ggcttcaaga tccgctggtg tgttcacatt gagaaatgat cggccttctg
     4501 gatcaatccg gcttaactca gccacgttga ttagccggac tttgagcgat ggatgtgcaa
     4561 cgatgtcttg gatcttcaag tgatgcgtcc ggaccatctc ctctaagacc ggaagacagc
     4621 gtcggctata gaccgcatgc atcggctgca agccgttctc cccctccgcc atgacgatat
     4681 ccgcctcctc cttcaggccg atcacatatc gcacagcctc ctggttgaga aacggcatgt
     4741 cacaggcaac gacgaagacg tgctcggtcg tggcttggct caatcccgta tagaggccac
     4801 ccaagcttcc gcagtccggc acgagatcgc gaatgacagc cgcatccgtc tccagagggg
     4861 gactatcctg cgcaatcacg ataagaacat cccgaaagat ggagcgaagc accgcaaggc
     4921 tccggtcaaa cagcgtatgc tccccgacgt gaaggaatcg cttatccccc cccatgcgcc
     4981 gactcttccc gccggccagc aaaatgcccg tcacatcggt aatcatgccg tgctcatatt
     5041 ccgtccgatt ccaaaaacaa ggagggggtg ggtttcccca ccccctccgt tgtgtctcac
     5101 actcggtctt ctcgcgaagc ttagagggtc ttacccttct ttttgttcgc gcgacgagtc
     5161 aacacccacc cttccaggag gaccacaccg aaggcgatca ccaccggata gatataggtc
     5221 tcttttggag tcgggggctg catgaacgtg tagtagaagg cagatacggc catcttgcgt
     5281 cctgcatcgc cattgtgccc gtcccaggcg aagaagacgg tggggatata ttgaccgacc
     5341 tcaaagaagg tatcttcatc gtaatcctgg tccttcttgt tccccaacgg acgctgaatc
     5401 atgacgtacc agcggccgtt cttccactcg ctcttgagaa ccttcagact ttcttcgtat
     5461 gtatcgcgct cttcgaagtc cttgtcccat cctgttcctt tgaaggcgcg gatggatcca
     5521 tcggcttccc acttcacgat atcgaccggg aactgctcgt tggtcccgaa caggtagcgg
     5581 ggcttaatcg gagcgggtaa ttccttccac ttcaccggag tctcaattgc gatcgcatcg
     5641 ttgtaaaccg cgtatttgtt ttggtttgcc gcgatggaac cttcctcacc ggtcttcgga
     5701 tcctgctcct tcacatcgac gttgacttgg gtcggcgccc agggaagctt gccctcggcc
     5761 acactcttgg tacggtcatc ccactcaagc aaataaacca cttccttgtc gttatagagc
     5821 gagcgcaccc aaatgtcatc gatgcggttc acgaagtttc gcggcttgtg ggtgatctgc
     5881 ccacccatcg cgacatagcg cttgggagtc ttctgccacg cttcattttc catatcggcc
     5941 ggaatctcgc cctccaccgg actcgatggc acgacgaagt tgatcttcgg cttgtcggtc
     6001 aatgggtcga tagggagggg ctttccatcc gtatcccgct cgcagaggaa attgacccaa
     6061 ttcgcaatat gccaacgttc ctcaatcgtg gtgttgtctg cgaatgaggg catcggggtc
     6121 ccgttcacac cggtcgagaa ggtacggaaa atgtttttca cattatacgg atcctggcgg
     6181 ctgccgcgga agttccagca cttgtgccag tctgccggtt gaatggcaaa gccccagtca
     6241 tctttcagat tgaacgcgtt gccatcgcca cggccttctg caccatgaca ctcaacgcac
     6301 ttcttttcga caatcagctc cgatcccttc ttcagactct cctccgtcgc agctatcggc
     6361 ttgagatcgc caagttggag aatcgtctgc gcctccgact gcttgtccgt gaatttccga
     6421 tccttcacga gctgggtcgt gacgaaggac agcacttgga gccgttgttc atccgtcaga
     6481 ataccttccc aggacggcat ggccgaccca ggcaatccgt gggtcaccgt ctcaaagagg
     6541 tcgttctgac cagggaccgg cttcttcgcg tcgaacaacg gaagctcacc gctcgccgtg
     6601 tgacggatct tgaaggttcc ctgattgaag ttacggggac gaggccagag acgatcggcg
     6661 ccaggcccat cgcctgcacc atccaccccg tgacaccaaa cacacttggt gaaatacacg
     6721 cgcttacccg cttcgatcat ctcggcagag ggttcagggg caagttcccc cttcttaaac
     6781 ccttcaggga ctgcatcttg ggcaaagagt gaaaccccgg tgctgcctga agccaggatg
     6841 actccgaagg cggtcgcgat taccactctt gccttccgac ttaaactgtt cttcatattt
     6901 accctcatct cgtatatcca ggttagcgat gaatcccgta agcgattaat cccaggtacg
     6961 gggatagtag ccagtatgcc aatactcgaa taggatcacc ttccagattt catcaaccgt
     7021 caggtgctgt tcccatggcg gcatcaccga ggcccagggg aatccttcgt tcggcaatcc
     7081 gataccgccc ttggctacgc gccagaaaat aaacgtttct tgcagttggg caatcgttcc
     7141 cggatcggta aagtttgccg gaatcgggtt gaatgcgaag gcgtgcaagc cgcgaccgtt
     7201 caagttgtcg ccgtggcaga agtgacagtt ctggaagaag atctctcccc cttcgcggac
     7261 gtacttcaag tacccctgat tcttatcatc ccagggattg gcattcggct tcattaagcg
     7321 gcccataccc tgttcgacga tgttcgcgtt ggtgaactcc tgatcgtact tgccttcagg
     7381 attgacgcga tacggattct gcgatgtttg caggacatag gtcttgccag ccaccttggt
     7441 gctggctgga ggagccggat gcaccgtacg caattcgatc ggctcttccg acttcggcac
     7501 catggcattg aaggaggtcc acccgaccag aacagggatg aggataagat acccatatcg
     7561 gagtagcttg tttgctccgg actgggcatc caacacattc atgatcggct gcttgaattt
     7621 tttccaatct tcttcattcg ccgacaccca cataaacacg gcgactaacg agaccgtccc
     7681 gtacatcgcc cgaacactaa aggggatcgg ggggtagacg cggaatttga gatagagcag
     7741 gacgaacgcc cagaaaccaa tgccctgcca gaaacggggg atggccatcc ccatcgaact
     7801 tgccatatag cttaagcccg taaaccccgc cacgaataag gcgaactcca gcaacatctg
     7861 aacgggcata tagccctcga ctaagcgcac agtattcgac gggatgacgt cgacgatcat
     7921 ccctactagc aggaggagtc caacgactcc aaccagcgcg ccgactgaca tcaatgcttt
     7981 catgggttca ctccctttag tctacaaaaa gatagacagc aaagattaag aaatcttcgg
     8041 aggcggcgca cccgccttta cctgcgacag ataatcaacg atcttcttca gtgctcccgc
     8101 gctcagcttc tgtccgaaca cctttggcat caggttgtca ggaaatcctt tgacaacgta
     8161 cgtactgggt gacaccacag actccatgat gtactctgga gtgctgtgcg cagtcccttt
     8221 gtacgccgga tccttgatcc gctgaggtgc gttcgttccc tcttctagct tggggccttg
     8281 ggtgcctgtg gcaccaggaa tacctggaat ggtatggcag accacgcact gtgcctttgc
     8341 gaaaatttga tcgaccggct ctgatccatc agccagtaag gcagtggctc cagcaggctt
     8401 gtcttcctgc tgttttggcc gatcggcttc gggaataaac ttctcgtacg atttgacaat
     8461 ctcttcgaac gacggagctt cacgcccttc acgaacatag agccacgtgt ccaccgctgc
     8521 cagctccggc agcgagagcg atattggtgg cttgtggatc tttggcatgg ggctttcttt
     8581 atcgttcgtc cccttcacgc cgtaaccagc gaccacatag cagctgggac aggaatgcga
     8641 ctcggcaatg tattcctgac cggtctcagc ggttccagca cctggaaagg cttccttttc
     8701 ggcatagtcg cgcttggaag gattcccctt ggaatatttg ggatcttcga gccgctcctt
     8761 ccccgcacgt tcgggaaggc ccaacaggtt tggcgcacgt tcgccgagca taccagcgtg
     8821 gaacgcgtgg cacagcgggc actgcccctt accgatggca ccctgaactt tgttctgacc
     8881 gatgccgcca aagatgatct tctcaccctc gtcggccagt tgttgcgggg tcattgaact
     8941 gaaatcgagc ttttcttctt tcggaggaaa accaccttca acctggggca gccagttgcc
     9001 gtagcccgag aggaaggctg ccacgaaaaa cataaatcca ccgattttca gcaaggcccc
     9061 tagattggtg aaatacaacg ccagcacgaa cgaggttgcg gtgatcatca cgagtgatac
     9121 cgggagaagc cggttctcac cgtaaaaatt gttcttatag ttcgcgatcg caatgaacag
     9181 caagaacgcc gcgataattc cgataaaagt cttgaacgtc gcgcgcttct tggcggcagg
     9241 gtcgctgatc gacacctgga aataaataag caaccccgct aacagggcta agaccaccca
     9301 ccccatcgac agggcttcat taatgagatt actcacggtt ttaacctcct gcggctaccg
     9361 ccagggagcg cgctttgggc acgctccccc aacgagccaa tgctcaataa aagatggtta
     9421 gtggctacca gccggctgcg gcgcgctccc gggaactccc gccttctcac caactgggac
     9481 cttcttcgcc gtcaagctac cgagccagaa gacgaacaga atgctgaccc agaagaacaa
     9541 cacgttgaat gaaatcatgt tggccgcaaa ccccacggtg tgggtgtagg cccatggtga
     9601 gttgtcacgc atgatttcgt tcacgtgcca gaacagacgg accgaggagc ggatgtagcc
     9661 catcaatccc atcatccagg taaacgcggt ggccagcatg atcagggcgt attgcgaacg
     9721 ggcggaaatc tttccccatt cgatcgggcc catctgcttt gcccccttca tcatcacgct
     9781 gttcagcgca aacatgaaga acagacaaga caatgtggtg gccacttgcg gcactgacag
     9841 cccgactcgc acgttggctg gaatgtagta cccataaatg gcgagccaga taatgttgac
     9901 ataggcacag gcgaagaaca cgcccatgaa aatattgccg aacttcgccc aagatacggt
     9961 ggagaccttg ttgcctcgca tgtaccatac gaagctcaac actgtggtgg taatgatgac
    10021 gttaatgccg ccgttcttag cggacatgac gccgtagtta cccagcaccg ggtgctgctg
    10081 accgcccata gccttcaatt cggcaggact catcaccatc gtgtgcggcg tgatgaatac
    10141 caagaagccg cagacaagga gaaatacgag gtacttgatg tagcgctgat acttctcggc
    10201 gcctcgcatc cggcccatgg cctgccacaa gtagtagttg gtgctcaaga agaggatacc
    10261 gatcatggtg gcctgaatga tgaaaagcca ggcaagcagt cctcccatga gcgtgatgcc
    10321 catctgttgg cgatatgcat agacttcacg catcagccag taaccagcga agggcagcgg
    10381 gatcaagaag gccacaccga gcgccatggc gatataaccc atccaatcgt aatgagcccg
    10441 atcttcctcg gtcttcgccg cgaggaactt ataggccgca taggctgcga cgacaccgcc
    10501 accgaaggcc atgttgccaa ggatgcggtg cacgttgagc gggttccaca acgcggtgtg
    10561 aatgacgtgc cagatgtttc cgaggaaccg gccctgctca tcgacaccgg ccggcgacat
    10621 catgaagccg atccacgaat tggccaggaa catcagtaag gtcccgatga cgttcaagat
    10681 gaccgacatg ctcagatgga tccacttgag gaatccttcc ttcatcttgt cccaaccgta
    10741 ataataaatg tagagggttc cgctctccgc cacgaacatc agcgcgtaaa tatgcatgac
    10801 cggccggaaa atgctggaaa gataaccgaa gaacgcagga tagagagtca ggaaagtaaa
    10861 gatcaggatg ccgcccagga tcgccgtcag cgaataggcc gtaagactga tcttaatgaa
    10921 gtcgtaggcc agctgatcat accgtttggc gagcgcttta tccttggtca caacccccat
    10981 gaactcgatg accatacaga aaatcggcac ggcgagcacg aagctgccgt agtagaggtg
    11041 ctgctggttg gcgaaccaga gcaacacccg gctctcgaag ttgtaccgcg gataatcttt
    11101 tggaccatcc gtggtcttcg gagcaggggc accgaccaca atgccttccg tcttgtaata
    11161 gacgtcacgc cccttctcaa ccttatctcc atccttcttc gctccgtcaa ccgctggagt
    11221 ctcctggccg gcagccagcg taggcaacga catgacgatc ggaagcagga ggaggccaac
    11281 catcgcgcag agcgccatga ttgagaacag tttctttcgg gctataagac ccatgggata
    11341 cctccttgat gcatttcgaa aaaaaatcac gaaaagtcca acagcgtttt ggggctattg
    11401 ccgcaactct acttgcggaa tagataccag taatccaacc cctgcatgtc cattaggaaa
    11461 tggtcgacca acgtgaaata ggccacacag ataatcaacg agatgacccc gtacacaatc
    11521 gactgaccca tattcgcccc tcctcagctc aaaataatgt tcaggaaatc ccggctcgtg
    11581 gccctgccta acaggggcac tgaatgccgg caaaccaata gaagaaccac agacccaccg
    11641 cggctgtgat atagaaaatc atcttgccga cttttacctt aagttcgctg tctggtgccg
    11701 tcgccgttgc catcgtatct ccttctcctg atgggaatgg ttaggtgagt gaattagatc
    11761 agggcattcc ttgacacgca cacgtccgtg tgttattcaa aatttgtcgt tgggcgcgaa
    11821 aacacaggaa aaatcatgac aaaaaactca aaagtgttcg acattatagt tttggctggt
    11881 atggttgtca atataatttt ggccgtgttt ttgatccttt actactttga ttttctttag
    11941 ttttatccgg acttctcctg ccgtttctgt ccctctgaaa tccccaaaat cgcacagcga
    12001 aacccgatcg tttcatcgcg gaaatccgga accattttgc tccggctggt gatgcggata
    12061 tcagccccgg tcgtggtgta gctcccccct cgaagaacgc gatatgtgcc gtattctggg
    12121 cttgggggat tctcctccgg cgagctcttg tagtacccct cgtcgtacca atcagccacc
    12181 cactccatgg cattgccggc tccatccatg accccgtagg ggctcttgtc ggcatggaag
    12241 cttccgacac gcgctgacac ctcgtgccca tcctgcactc gagcccaatt ggctccattg
    12301 ggttgctcgg catttcccca gggccagagt cggccatcgg aaccgcgcat cgccttctcc
    12361 cattcagctt ccgtcggcag ccgtttgccc ttccagcgac aatattcgct cgcatcgtcc
    12421 cacgacacat agacggcagg ttgatttgtg ccacgcattt tcccaatact cttcgcatag
    12481 cgcgaaggcg gaccagcctt gcgatgtccg gtggcgacga cgaattcctg gtattggtga
    12541 ttggttacct cgtatcggtc gatggaaaac gtttcgagga aaatcgttcg ctgcggctgc
    12601 tcatcgaacc cgccgctcgt tgtcccacgg agaaaaggcc cggcgggaat cgtgaccatc
    12661 tcttctcgaa tcggttcttc ttcgggcgcg gcctccggtt cggatcgagc cgtttcgatg
    12721 gctcccggcg gtcccgcttc aaacggcgtg agcgtggtgc cacgaagaat gcctatgatc
    12781 ggaatcgcgg caaaggctag aacggtaaga agaaacgcaa ccttgaattt ggtttccagc
    12841 ataccggctt aagaagagga atcgctgttt gtgccatcca gatcgctggc gcagcgaatg
    12901 ccaatggtaa tgtcggttcg ccaatgcttc gccgcaaatc gtttggacag acgggcattg
    12961 tgctcagtct cccgccacga cccgccacga acgaccttca aatctccggc cgtcggccct
    13021 ttcggatcgc gataaggcga tttcttgtag tagctctcgt catacgtatc cgcgacccac
    13081 tcggcgacgt taccggtcat gtcgaaaatc ccgtacggac tccttccggc ttcgaacgag
    13141 ccgggaggcg ccaggtactt atatccgtca tcgcttccgg cgacattggc cgcgtgtgcg
    13201 gcaaaccttt ccccccacgg atacttccgc tttccttcgc cgcgccccgc cttttcccat
    13261 tccgcttcgg tgggaaggcg cttcccggcc cacttgcaat aggccactgc ctcatcccac
    13321 gacacactca tggccgcaaa ctccggctgc agcagcttgg attgatcgtc ttcaaaaact
    13381 tcgatccttg gcatcggacg ctttgtcatc ttggcaaatc gcatgtactc atcctgcgtg
    13441 acttctttgc gatcgaggta gaaacctttc aggaaaacct gatgctccgg cacttcgtcc
    13501 ggatcaccat cctgactgcc catcgtaaag ggcccttccg gaatctgcac catttcacgc
    13561 ccctcatcgc ccatttttgt cttatacatc gagtaatcct gtgcaggagc gctgctcacg
    13621 gtcggccggc tttcagtctt aatggactgc gccagctcct tcattttata ggccttgttc
    13681 gactcccaga gcatcaggcc gatcatcagt atgaaggagc cgaacacgaa gatgattgat
    13741 ccaatgagaa cgcccttatt ttccataacc gctcattctc tttcactcat gcttcagact
    13801 gaaggcccag actgctcagg cgacgtcttc cgtgccgcgc gcaggtccaa gagcattgaa
    13861 atctgcacac ccagaaaccc acccatcgct gctccggccc cggcaccgac ccaaattcgc
    13921 tcgtcgcccg ctacgagcca ggccagagcg ccccctagta cggttcccac caaaatgctc
    13981 acgagaaatc gtcgaccgcc gaggaatccg atgacgccgc ccagcgcgag gcccactccg
    14041 attgccacag gcaccaaccc gccgcccatg atgtatccga tcagagtccc cacggttccc
    14101 ataaccgcca acccgaacac catatcgaac accttctgaa gcggcatgga actggcttgg
    14161 gtcgtatcca tgaaaaacgc tcgcctactt tcccgacatc gcgatcatcg gaccaaaatc
    14221 aatttcgaca ccaggcccgg caatgatgga aatgccccag gccttcgctg ccggctcgtg
    14281 ttgatgaatc cgtttgaagt cttcgtagac gttcactttt tcttcaaacc actggcccac
    14341 gtcttttgtt ccgtattgca ccacgatttc tgaaccgctg aacatgccac cttcggtcaa
    14401 cgtaccttcc ggctttgtgg aactccagat atatttcgtg aacaccggaa tgaacaagag
    14461 atccgtgtcg agcgaggcat agatggctgc aaccggttcg gttcccgttg cagccttatt
    14521 caaccgccaa cgccaggtca ataccggata tgccttggga tcccagtcga ttttcttttt
    14581 cttgatccgc tgccctgcat ctttggcgga taagaaattc gcgccgccct ccgtctgcac
    14641 cttataggcc tcgcgcccct tggattgact gcgctggttc tcatgatccc aattcgaggg
    14701 aaagccgtcc gcttcttttg cctggaagtc ttcgagcacg agcgtccgcc cctgcgcacg
    14761 agccgacccc tccatcaccg cgatgcttcc cgccagccac gacatcgccg ccaatccaca
    14821 aagtaccagc ctgctcttca tcggtccgat caccctttct ctgccgttac acttcttgcg
    14881 agggagccgg cgacagcgcc ggagacttct cctcgaccaa ctcttcgccc atcgccggtt
    14941 gctgcccacg ctggcacatc cagaataagg caaacacggc gccccagaac accaccatat
    15001 tgagcgtcac catttttgcc gcaaactcca gatccggcgt gaaggcccag ggggatacgt
    15061 ctgccatcaa ttcgttcacg tgccacgaca accggccgga ggaacggata tagcccatga
    15121 ggcccatcac ccatgtaaag gccgccgcta atccaaagag cccgaccatg ccgcgaacgg
    15181 gaatcttgcc ccattggact gacccgtgaa gggttgcacc cttgagcatg aaccgattga
    15241 tcagcacgct ggcgaagatg accgtcaacg tggtaaaggc ctgcggcgat gaaagaccga
    15301 cccgcacctt ggccggcagg tagaacccgt agatcgacaa ccagatcaca ttcaggatcc
    15361 ccactgcaaa cagccccccg atgacgatat taccggttct tacccaggag actgtcatcg
    15421 ttctatttgc ccggcgatag tagagaaagc tcaatgtggt gaggaggatc atcacattga
    15481 tcgcaccgtt cttggccgac atcacgccgt aattcccgac gaccggatgt tgagccccac
    15541 ccatggcttt cacttcgccc gccgacatca tcaccgtatg cggcgtcagc cagatgaaga
    15601 gcgccaacgt caaggcaccg agcagaagtt gataataggg ttgataccgt tcccctccct
    15661 tgatccgcgc catgctttgc caaatatagt agttcacgcc gagaaacagc gcgccgatca
    15721 acagcgcctg gacgacgaac agccaggtca gcagcccgcc catcatggtc acgcccatgc
    15781 tttgacgaaa agcatagacc gaccgcatga gccaatagcc tgcaatgggc atcggtagaa
    15841 gcgcacagac cgtgacgaac aggaaaatgt atccgaccca gtcgaagtag gcccgctctc
    15901 cttctgtctt gctggtgaag aatcgatagc aggcataggc cagcacgacc gccccaccgg
    15961 acatgatatc ggcgagaaag cggtggacat tcagcggatt ccagagggca gaatgaagga
    16021 gatgccaccc gtttcccaaa aaccgtccct gcgcatccac accggccggc gacatcataa
    16081 aggccgccca ggcattggcc agtaacagca gcgtcgttcc aaaaatgttg gtcaggatgc
    16141 cgatcgccgc gtggatccac ttcaaacccg gttccgccat ccgattccaa ctgtaataat
    16201 aaacaatgag tagtagcgac tcgccgacaa agatgatcgc gtaggcaggc atgaaggctt
    16261 tgaacgtccc gcccatatat ttcatgaaac tgggatagag aaaaatgaac atggccagca
    16321 tgaggctgcc cacaacggcg gtaacagaca acgccaggag tgcaaccttt gcgaggtctc
    16381 ttgccaggcc gtcataccga agcgacaagg ccggtttctt cgtgatgagc cccaaaaatt
    16441 cgagcaacgc acagaacaac ggcaacgcca gaacgaatcc gccgaagtaa gtgtgttgct
    16501 gcgtcacaaa ccacacaaga agacggctgt cgaaggagcc gatccgggga tagaccgtct
    16561 cggtcggaga gggtgccgcc ggcccttgcg gaattccttg cgtcttgaag tacagatcag
    16621 cggcgtcttc ggcaaacgcc gggtgctgcg cgccaggacc aaggacaaag agcgccgcaa
    16681 gcaatgcgag cgggacggag agcgtgaaat atctgagcat ggtgaattgc ctggccatag
    16741 tgttagacct ttgatgcagg ggcagcggtg acaccggcgc ccatatttgt agactttcca
    16801 agaatcccca tcacgaacgg gcaccggagc aacgcattgc tcaatccttg tccctgttgc
    16861 tcttcaacct gccggcgata ccctagatag taaccgtaga gcgccatcag tccggcaacg
    16921 agccctccac cgaccacggc gtaggcggtc gggattcccg gctgcctgag caggaagatc
    16981 acgatgccgg ctgccacgag ataataggtc gacgaaaatg cgagaccggg ccaccaccag
    17041 tatttgagca actcgctcgg cttggtcttg agcaatcccg ctgtccatga ctcgttcgga
    17101 cggagcccgc cgaagtaggc acagagcgcg atggcgcccc cgagcaacgt cacagctgac
    17161 aattgaatga ggaccgcgac ctggctgttc tccgtgatgt gtcccagcaa ggcgcccgtg
    17221 gcaccgacaa tcagacctcc gacgatccaa ggaccgactt cggcggtgcg acttccgatc
    17281 aagcctcgca aggcgacacc gttgggaaac gtcggaaacg gccggcccat gagaccgatt
    17341 ttccgaacaa ggccgatttc aaacgcatcg cgggcgacag ccgtacagga aataatgatc
    17401 gccatcatgg ccgggccgtc cggatgttgc agatagtcat atcccacgag ccacaacagc
    17461 gccagaacaa gcaacgagag ctggatgccg tacacaaacc cgatccagcc tgccatccac
    17521 gttaacagct tgatgccatc ttgatgactt atcacgcgcc aggcagtcct gcgacctact
    17581 cgataggcca acaaggcggt gattaatccc acgatcccgc tgaccagcaa caagaccacc
    17641 ggctctgtca atggcagcat atgcttgacc agtcgataga taccgaatgc gacaagcccc
    17701 ggaatgaaca tcacgattcg cttctgcttg cgaacggaat cctccgtcac cgcgaggctg
    17761 agactcggca agactcgtaa cgccattcga tgcaacattg ttaatccgtt agacgtgaaa
    17821 cgtgaggcgt taggccatgg gaaattttca cccccttacg ccttacccct tacgtcttct
    17881 gagccgccat cccaaaatac ttggccttga tttcttccat cagcgccacg gtgattcggg
    17941 tctgcccgcg gtccgcggcc gtgcgttcca actcaatccg ggccatcgcg cgcaccggag
    18001 ccgggacatt atccagccga cgctctgctt ccggctccca ctcaatccgt tctccggtac
    18061 gcgggcgaag cagcgtgtca tacgtgatca cccgttctcc gcgcgctcgc acgtcctgtt
    18121 caacttcctc tcgaaacaat ggagccagat acggcggcat ccggtccaac cgatgcagcg
    18181 catcatcggt ccacatgacg cgatcgacaa atccgttcac ctgacccgcc ggcacatcca
    18241 ccccaacctt gaagccgcac ccgcggcact cggatctgat gaaccactgc aacgccccat
    18301 cctcgtaggc tcgctcttcg atcccttcag tatgcatcca acgtccgcac ccgcaggtca
    18361 gcatttccaa caccaagttc tgagtgctga gtcgcgagtg ctgagaatca tgtgatagcg
    18421 ccaactctgc atttcactcc attcaacaag tctcagcact cggatctcag cacttcttgt
    18481 gtcacccgat ctttcccggg tacagaatgt agagcatcgt gtaggtggta gttccggtga
    18541 caaataggac gcaatagatg atcatcgtaa agcgtccgag agtccgatgg cgttgcccgc
    18601 cgtccggcca tatacgctga atcgccattt cgacggcgaa tacaatcgcg atgagggcga
    18661 gaagtccgac ccacacaaca agccgtccca tccgaaaacg atctgctgcc accaggtaca
    18721 gcaagtacac cgccaccagc gcgcagagga caccgaagat catgccgatc ttcccccagg
    18781 tcgtctgaag gatgcgaccg ctcaggatgc gtcgcccttg ctcgaccgcc tgggcacgaa
    18841 accccagcac aatcatatag accgccatga ccaatccaat aatcacaaga atgatatgaa
    18901 aggtgaggag agggatgaac acattgtgat aaagtgcgtc cgagccgccg aaaccttcct
    18961 tcccttcaaa ggccagcacg cccaattgcc ggaacagata gtaactgacg aagaacccga
    19021 gcatggctac catgccgccc agcatcagcc agtggtgagc atccgattgg cgcttccgtg
    19081 cctggatcca gccgatcacg aacaggcagg tgaagaaggt cgccatgagc tgactgatat
    19141 cggcgccgat cgtggcgtgt gtcccaaaga agcctggatt ctttaaccaa tccatttgtg
    19201 cggttacctc gtaggtctca tcccttggac gaccgccgtt cgttccaatt caatgactga
    19261 aacaaatccg gctttcgtca tccattgcat cgcctcagct gttttgtaac agccgccctg
    19321 ttgcgtattc accagaatgt gcacggcaaa cgccgtagtc caggccggac cagttcctgc
    19381 ctcatccagg aatcgatctt tgatgatcaa gcgtcctccc ggtgatactt gcgtaaagac
    19441 tttcttcacc aaggcttcgt ttgtctcgaa agtctgatag tgcaggatgt cggacatgag
    19501 cacgacatcg tatgggccgc cgagcggatc tttgttaaag tccccgggaa gaagcgaaat
    19561 tcgcgactcc aaccccgcat ccttcaccgt tttctcggtc aaccgcagcg tggcgggaag
    19621 atcgaagacc gtcgcagtca actcgggata gatgtggcaa aacgcgatgg cattggttcc
    19681 cgcaccacca cccaagtcca acatccgcac ccgtccggcc agctgcagcc gtttggcgaa
    19741 gtcagggccg ctctgctgac cgatgcggtg aaggaccgca agcacattgc tgcctatttc
    19801 aggatcggtc tcgaacacat gctgatcgac cgaccggtgg ccggatctga tcgtttcttc
    19861 cagtttcccc caattgttcc actcggcatc gtgcaacagc agcaagtgcc cgatgtactg
    19921 gggcgagtga cgaacgagat gcttctcagc cgccaccgag ttacggtacg tctccccctc
    19981 tttggccagc aatcgcatgg ccacaagggc attcaaaaga agagtcaggg tcggttcatg
    20041 ggccccgatt ttccccgcaa tttcccgcgc tgtcctcggc tttccgtcca gcacagaaaa
    20101 tacgtccagc ctcacggccg tcaggagaat cttggtctcc cagtagtaac caagctggaa
    20161 aatttctgcc agcgagagtt cccgcgacac acccatcccc tcaagcgaaa tatctttgaa
    20221 agtcggcatc ttacctagat cggaaactga aaggcaaggc ggcggaagct gtggaacata
    20281 gggcaaaaac acaaagggca gcggattgct ccgctgccct ttgacggcgg gttgccccgc
    20341 caaatacgaa atgcggctta cttgatttca gccttgaggt tggcggttcc gccttccggc
    20401 acttcaactt cgaattcaat cttcttgccc ttggcggcaa acggatgcca ggccacgacc
    20461 ttgtgcttgc cggccggaac atccttgagc tcgaatgagc cgtcatcctt gaccactgcg
    20521 aagtgggcat tggatacagg gaggaagaag gactgcatga attcatgctg gtcgcactgc
    20581 aaccgataga aaccatcctt ttcggcgccg ccacggaaca cgaccggctt gtcgagctta
    20641 tcgcccttct tggccaaacc gatgttgaaa ccggtcgcag agctggcacc cttcaccaca
    20701 aagctgtgcg gattgtggag cacaccctga accgacttgg ggtcatccgg atcggcatca
    20761 gtgttctcaa ccttgaagga cttgttgttc acaaccacgc cgctaaaggg caggaactgg
    20821 cagaacgccg ccgtcacttc cgtgccggca tagccgtcca tgaatggctt gtcttcgata
    20881 tcagacacgg ccacgacggc acccttgagg ccgccatcct taccgacttc aatggtcttg
    20941 aggaagcgct tgtctccatc catcaaggtc ttgtccggga tcttcgggca gaacttcggg
    21001 ttcgggaact tcgcgaagga gaattccttc tgttcagact tccccgcata agtgaccttg
    21061 ccggcaatgg ttccgccagc aaacgaggtg agaggcgccg cgacgaacgc gacggctgcc
    21121 acgccaaata caactgataa cgcactcttc atgggactgc ctccttgtga ttaacgattc
    21181 gccctgattc ctagctcgct ctttcctccc ttgatctacc gacctgttga gagaaagatg
    21241 ggaactgctc cctctctggc ggactcgatc gacgacatgc tggcgaggct ggtctgggac
    21301 cgagtatgta tataaaatcc taccctaccc tgtcaacgtc aaacaagggg ctgtaaagga
    21361 gcttccaggg cctatttctt gcgctgcatc aggtgaagca gcgcgccggc tgctgttgag
    21421 cgtactgctt cattctgatc gtgcagcgag cttttcagga agggaacaac ctgccggtcc
    21481 ccgatatgac cgagcgaacg aatcgcgacg atcttgggtc caggcagagg atcagctgca
    21541 agattcgata acatatcgac tgcacccggc acattcacac ctgtcgcttt cccaagcgca
    21601 aacgctgcgg atgatctggc tgcggtatcg ttctgttgcg cgagagcccg tacggtcggc
    21661 gccacggtct caaaaggctg accaagctgg agcaaggccg ccaccactgc ggcacgtacc
    21721 gtcacgacgc cgtctttcag tgcctgattt aacgccggaa gggactcctt ccccccaaga
    21781 tcagccaagc tcgccgcggc agactcgcgt acggcaggaa ccggatcctt gagcaactgc
    21841 tcgatcgaac cacgcgcctc ttgcttttca aggtgaccga gacctcttgc cgccgcagcc
    21901 cgtacggaag gttgctggta cgtcagcaat tccatcatga cagaggcgcc gcgctggtct
    21961 ttgaggtcgg caatcgcccg aatcgcctcc gctcgatctt ccggattggg ggcctcgact
    22021 ccctgtcgca actgctccca cgcgtccttg cggcccagcc tgatcagtgc cccgtaagcg
    22081 gctatcctga cgggggtcaa ttcatctttt gttgccgctt caataagggg catcactgat
    22141 ggatcaccgg actttcccag cgctttgacg acgcgcgcct tcacaagacc agcctggtca
    22201 tcgagcgcag cccgcagctt cttggatttg cgacctgcct cagtacggcc taagccctcg
    22261 accgccaaca cgcgcacagg cccggatcca tcactgagtc catcttcgaa ataggggacg
    22321 atgtcgtcgg aatcgatttc tttgagcgcc gtataggcgg cgccgcgcat ctgttcgcgc
    22381 atgtccttca tcatgacgac gataaatccc agagccacct cgcgtagaac agactggtcc
    22441 tcctgcctca gcatcgcttc gagtgcgtca tattcctcga gcgcttcctt gggcttaccg
    22501 agcttgagga gggatcggat tttcagcctc tgcacctccg gagacgcagc ccgttccttg
    22561 tccaacttgc ctatctcatc cagagtctgt tgaaactcat tcttgtcata gagattttgg
    22621 attggtttcg gcaccgtcgc cgtccctccc gcaaaggcag gcccagtcgc aacaaacagc
    22681 cagaagagcc cgatcacgaa gctcacccca agtccaacgc ttacgttcct catcatcatc
    22741 ctatccttgc tgtcttggac cagcggcgcc gattcgtttc ttgtagccgg gcggttcatc
    22801 gaagtagtac gcaggctgcg gggacagcct gatcctttca tattcgaacg accagtcgcg
    22861 atcccggctg accagcttga ggacgatccc gacttcttta tcgacccatt cataaaaccg
    22921 ttcggcacga ccatgacgat ccgtctgaac ttcgtagagt tgcgctgtgc gaccagccgc
    22981 tgtcgcgtcg ccgaccaaca tccggtctcg ttcccccgga agcccggctc tcatcggcag
    23041 gacatcatcc ggatccaacg gaaccacgag cagttctttt tgttgcgcaa gaagatacca
    23101 ggtctccgac tggtccagac ggatgatttc gatggctgca tacccatgct ccgtccgaat
    23161 cgcatacttg tactccaggc gcagacggtc ccccttggcg aacacctgcg cgtgatactt
    23221 ctttccgttc acctgcttga tgatggtgcc cgaaaactcc ggttgaatgg gaagtgtatc
    23281 gccggccatt gcacgacggg ccggcgacca gattgacaag gaaagagcaa gcccgaggaa
    23341 gaccagtacg cgggtgctcc tcgcgaaacc attcctcatc ggctgtcaat gctgatgttc
    23401 gaccagcggt tggatatcga ccggataccc cagcgtgtcc gggccaaaac gaggattatc
    23461 catcacctta aaagcagagc gattcccctt gggaggattc aaggacagct gttcgaccaa
    23521 tttcccgtcc ggttgaatgg tcaccatctt cgtgacgccg ggaccggttt gaggatgcca
    23581 cacgaccagc tgataggtgc ccggaggaat gttctcgatc ttataggctc cgttggcatc
    23641 agtgagcgca tagtacgggt tattcaccgc catcgcccaa ctttccatgt aggcatggaa
    23701 cccgcactgc atataaaaag tccgccggcc cttgttcaaa tagatcggcc cgaccagcga
    23761 cttgcccgga gcatggttgt gtatcgcgtg aagatccccg cgtcgatgct gatggttcat
    23821 caccagggga gtattgaaga gcgtgcgagc ccccgcctcg atcgacgtct cgtagccctg
    23881 aatatcatgc atcacaggat ccatattgat cacttccacc gcgtggccat tgcgcacgat
    23941 cgtcatgaac ggctggaaca tgcaatctcg cgcctcaatc aaaggcacgg acatttcaaa
    24001 cggcttgccg gcttcaaccc cttcgagcaa cacgatggca tctttgagtc caccttgagc
    24061 atccacgacg aaatcgtgca gcaggcgcca gccgcgaccg ttcgaaatgc gcccgcaata
    24121 ggcaggatca ggaaatgtaa tgagattgaa tccttttggc tccggcaccg ccccatccag
    24181 gaggaccgtg ccctcgatca tcccgccgtg ctgcacatca atcacgtcat aggccggcgc
    24241 gggcccgacc gtcagcacaa cgagaatcgt tgaaagagcg cccagcagcc ctgtcatcaa
    24301 tttgtcgttc atgcgtgcag ctccttatgg ctcacggcac ctgaagttcc aacgtcggtc
    24361 tgatgtctac ggtttttccg agcgcctgaa gcccgaagtg gggattctct tcaatttcat
    24421 gagcgctacg gcgccccttg ggcgattcaa aaagaaaatc ggtttgaacc gccccctttg
    24481 ccggcacggt gatcttcttc tccaacacgg tgccaacccc tggatgccat gcggtcagca
    24541 gatagtctcc cggcggcacg tcagagagcg aaaacgtccc gtcactgccg gtgacctgat
    24601 agtagggatt ctcgatcgcg aggccccaac tttccatata cgcatgaaat ccacattgca
    24661 tgacgaagaa tttcctcccc ttcgtcatat gtatcgtttc tgtgaccggc tggccggcca
    24721 aatgctcgtg gctctcggct ccgacgcttc gcttgtgatg aggattcatg ggaaggggcg
    24781 tattgaacag cacgcgcgga ccaagatgcg acgtctcgta ggcttgtata tcgtgcatca
    24841 cgggatccat gttcatgatc gtcacgtcgg ccttgtcttg caccacggtg acaaagggta
    24901 aaaatcggca gtcacgcgcc tcgattgttt gaggctcgaa cttgaacggc ttccccttcg
    24961 tcacatccga cagccacacg accgcgtcct tcagcccgcc gttggacgcg acctcgaact
    25021 ccttcagaat ccgccagcct gttccggtcg aaattcttcc gcagtatacg ggatcgggaa
    25081 acgtaatcag gttgaatccc ttggggacag gtttcccggc cgtgatcgtc accttcccgc
    25141 taatcgtacc gccgtcagtg acgcgaattt cttcgtaggc ccaggcaacc gaggatgtga
    25201 ccgccagcca cgaacacagt cctatagcta aggcgctgtt cattccatcc tccactccga
    25261 tggcttcgga tcattcatgc aggaagttct gtatcgtacc aaaagcttgc acaaaagaaa
    25321 aagggggaag ccgttggctt cccccttctc cttgttgcac gccctctcag caggagggcg
    25381 tcatgttact ttgctgcaac gggaatcgcg tgcgctgctt cctcaagcga agcgctcttc
    25441 atccctgcac cggctccggc aagaggcaac aaccgctggt cgcctgccgg caacacgcgg
    25501 agatagcccc actggccaga ctgcgagtac ggcaaccgct ggttgctgta cacatagtcg
    25561 cccggcaggc gatagggtcc gccggcggaa ggaatgaagg catccagcgt ttccgaaccg
    25621 gagtattcga ccacgctgat ctggtcggct ccacgcatga agggctcgat cggccactca
    25681 tgcttctcga cactgaacat cccgttctgt tcgttgctgg caccgatcac atggatacgc
    25741 acgggatcgc ccgcatgcga ctcaatgatc ggcgtcgcag gatcttccgg cttgtccgct
    25801 ttgcaaggct ggaatacctt gcccagcgaa cagccctgct cttcacgata cttgtacggc
    25861 tccgaccgat aattcacgcc ggtcagacca gccacgtttt gcacatacgg catgaagctg
    25921 gtgccgatga tattgtcttc gtcctggaag aacaaggcca catcgcgata gttcggacgg
    25981 gtctcgttcc ccggcaccga gcgatcgatg atcacgtcgg cggcccaggc attcttgttc
    26041 gagaggtccg cgccggtctt cgggtcacga tacttcgaac ccttcggacc gaccacgacg
    26101 gctccgaaca atccgttgcg aggattcgtc atcacattgc cccagtccca caccagcgaa
    26161 gtcgtttcgc cgttaaacgg atccgcatag tacgtgtagg tccgttcacc gcctggagcg
    26221 atcgtctgat cgccggcgtt gttcccgacg ttggcaccca gcgagtcctt cggatcgaag
    26281 gccaagccga tggccgagaa ggatgccttg ctgtccttca tcttgttctt caagttgact
    26341 ttcacgcaat ccccgacatt cacgcggagc gtgagcggca tcggttgcgc tcctccggac
    26401 accttggcgg catcttcctc caaggcatag atcttcgcat ccggattggt gatctggatc
    26461 ttccgctcga agtccacttc gatggactcc ggcgccttcg cattgaactt catgccggga
    26521 taatccagcg ccacgacgtt gaagctcttc accggcgcgt cagctggaca gaccggcaac
    26581 gccttcggca tctctccctt gctggagaat ccggccggca gcttcttcag atcggcttgg
    26641 tccttgtcat acacacgaat gatgccccag ccaccctcgg agaacttcga ggagcggccg
    26701 ttgaagtgga tgtagtcgcc ggcttgatgc cgcgatccac ccgcctccgg caccacgaga
    26761 tcatagcgtt ccgcgatccc gatgtgaatc gagttcttac ggttcgcatc cgacgcatac
    26821 cgctccgacc agaacgtatg gccggagatg gtccacacca tcgattcgtt catgagggtg
    26881 tgcaggagac gaaacaccat cgtgtctccc acgtatgccc tcagtgtcgg cgtataggga
    26941 tcgccgtgga tctggctatt gaacgcttgc gacacgtcag gattcgtcgc caaccgctgc
    27001 gcaattggtc ccgccctgaa gttcaagccg ctgccggttg tatgggtccc gccgttcagg
    27061 aacggcatcg gcgtcatgta caatttttcc ggcatcatga aggacacggt cttgcctgcc
    27121 tcgagcgcca cttcaaccgg ctgcccgggc ggattacctg ccgtcacgat attgacggta
    27181 tgcggcacgg tgtcgtgcac ttgcaccatc aattcgcgga agctgttgtt cacgccgtgg
    27241 cccaccggct cgttggtgtg aatatcagca atcggtccgc ttcggatagc ctttccggtc
    27301 ttcggatcgt gataggtcga ccccactggc tcggcgatga acgtaccgaa cccgccgtgc
    27361 ggccaggtgg tcgcgccgaa tgcgtggtcg tgccagaaga ccgttcccac atccgcatcg
    27421 acccagaacc gttgacgaac gaactccacc gtgacgatgt cgttcgccgg gtgatcgttc
    27481 ttgagaccct tcgccaaggt gatcgcgttc cccttgatcg ccttgatccg ggacacttcg
    27541 ttgcccttca cgttatccgc accgacaaga atcagcgtgc cgacatggta ctgaccggca
    27601 ttctttacgg tgatgctggt tgcgcccttc ttcgccgcgg ccgtcaacac cgtgttcatc
    27661 ggagccggaa ggcctttctt ggtcttcttc tccagcatcg tgaacggccg aaccgactgc
    27721 tcgtaggaga agccggtgat gacgccgtca gacgcctgat tgtcgaactg caggaagtgc
    27781 cagtgggtgt taatcttcga cgactggaag ttcgtgtagt cgtcgtcgtc ccactcgctg
    27841 gtcaacgtcc aatccaagca atcatagatg ttgccgcgaa cgaccaacgg atacttcagg
    27901 tcgtcgttgg cacggatcgc cgcctcttct tcgtggagca cgtaaatcaa gccgttcgga
    27961 tccaccacgg gtggctcttt gccctgtgcc ttggcgatct tgatcggcat cttcacgaag
    28021 tgcacattgt aatgcttccg gcccgcgttc tctggacaga gactccagtt gccgttttct
    28081 ccgggcaacg attgatccac actctcctga ccgtccgcat cccggcggat catctccagc
    28141 cagggcgcgc cgacgtggtt cggggagaac atcacccgtt tcccgaagtg cggcgtcagg
    28201 tgcggccatg ccactttacc ggtcaacggc tcgaacatga tcgggtgccg cttgccggga
    28261 tgggaggact tgtacttggg attgtcggtc gtgctctcct tttcgctcat cgccacattg
    28321 ccgttccagg tccaatccaa cacggtggcg tcgtaggaaa taatctggcc cttctcgtca
    28381 tctttgtggc cgggcttgcc catcgtcggg agctgcatct ccacccagtc cttaatggtg
    28441 atcgtggccg ggttggactt ccagtcactc ttgcccttct cgacgatctt gaaggctttc
    28501 ccaaaccaat cgacggtctg gccgatcagc ttgtcggatg aaatcggggt cttgatgcgg
    28561 cccttgcgat ccggcagctc acgcaaatcc ggcatcacgt cgttccgcaa atccccctgc
    28621 tggatcgtgt tgtacacacg ccagtagccc cacatccccg ccacataatg gtgcgccacg
    28681 tggcaatgga agaggaagtc gccggccagc tgctggcaga gaccggaccc gcactccgtt
    28741 tccaggtcaa gcgcttcgga cgggccaata acttcaacgt cgacacggtc tgtcttggca
    28801 cggatcaccg gatacttgac aggaccattt ccactggtcg tccagaggtt catgtcatcg
    28861 atggcccgcg ggctgcgcgg ccaacggatc gtgcctccgt gcggatgatg gctgtggaat
    28921 acttccgatc caccgtgaac caggcggaac ttcgccgggt cgccgatata ggaacgagga
    28981 atcgtcgggg acggatcgcc gaacgtatag gagctatacc ccatcgactc gtcttcgaag
    29041 ccgaagtatt cgtgctgcac gtgcatgtta ttgatgccga acggctcgct gcgatagttg
    29101 atcgcacggc cgcccggacg ataggcgtcg gtcagcggat cgcgctgcgg aaggaaatcg
    29161 cccttcttat tgagcgggcg gaacgcctcg tcgccgacct catgatagta gagcacgaat
    29221 tcccggaagt ccggacccgt gccgtttttg atcatgacct gccagccgct ggtggccggg
    29281 gtcgcctcgc cgcttccgag cggttcccaa taggaggagc cgcggggttc tacgacgaac
    29341 gaaccgaaga gacccatgac ggtcaattca cggtcgttgc tgaaggtatg gaactggcgg
    29401 acaccctctt gagttccagg gtgaatgtac cattcaaact caccgctctt gtccttgacg
    29461 actatcgagt ccgggttggt ggtcgctgct gaagcaccgg ttgcgctcac caccatggct
    29521 gaaccatgga tgtgaaggct aacgtcttcg ccgccttcca acttgttgct gagcttgatc
    29581 ttgacgcaat cgccttgatt cgcacggatg gtcagcggct gaatccactg tgcctgcacg
    29641 ccaggaatca cggcaccagg atcatggcct tccttctcgc gaccttcctt gttcttcgct
    29701 tcttccgcac gcaccttatc gatattctcg tccagcacgt acatgtagcc gggatagaag
    29761 tcgagccact ggttcaaggt gatctcaacg ttgatcgcgg acacgttgta ctgcttgacc
    29821 ggcgccgtcg cggggcactt gccgccgctt gtcatcgcga ccggttcgac acggggatcg
    29881 gagacgagga gcatgtcctg tccacccgct ccatactggt gcatcatgga ctgggtgtta
    29941 tacatgccgg tattcacgcc ctgaacctgc ggatcattgg acatgtggtc catgatgcgc
    30001 tggtgttgct gctcgaccat cgccgaccga ttggccttcc cggagagcga gtcttcgacg
    30061 atcgtctgtc ctttgagctg ttccgcccag gcagggagtt gatgattcat cgccacgtgt
    30121 gacgatgagt tctcagccga tgcgagcggg gcccagagca gagccaccgc tgccatcatg
    30181 ccaagcatgc gggtgtgcac acaaacccgt tgcgtgtgag aaaacatgga ccggcctcct
    30241 tgaagatggt gagtaaaaca accgaaaacg aaacgttacc gaacgataaa cgacctcgcg
    30301 aaattaattc gtatcaacgt cgagccaaaa aacccgcttt catcgacgaa ggaactggaa
    30361 agatacttaa gttcacgggg actgtcaacc ccaaacccga cagatcgatt gacacctctc
    30421 ttgcaagcct ttcaccttat aatgacgttg gtggcgtaca gccagttgag tcttttgccc
    30481 tattggttac aaggacttct tctcctgtac gccgctgccc ccatttgtgc gataatcttt
    30541 tcatgctgct gaagcgctgg cttgtaggcg atccactcaa aaccgctcaa gcttctcacg
    30601 agcgactctc caagaccctg gcccttgctg tcttttcatc caatgcaatt tcctcagttg
    30661 cctatgcgac ggaagaaatc ctattggtgc tcattctggc aggcacagcg gcaatcgcct
    30721 ggtcgattcc cgtcagcctt gcgattctgt ttctcgtcgc agtcctgacc gtttcctatc
    30781 gtcaaatcat ctatgagtat cccgagggag gcggcgccta tattgtggcg cgatcgaacc
    30841 ttggcgattc acctgccttg attgcggctg gcgcgctcat gatcgattac gtgctgactg
    30901 tggccgtcag cgtcgcagcc ggtgtcgcgg ccctcacttc cgcgctgccc agcttgtttc
    30961 cccatcgcga agcccttggc ttgattgcca ttctcttcat tgtggtgatg aacttgcggg
    31021 gtgtcaggga atccggaaag tttttctcca tccccactta ttttgcgatc ggtgccctgg
    31081 gacttatggt ggtttcaggc accatacaat ccctgctcaa ccccagcgtg gccccatcga
    31141 tgccgacccc gtccggcaca tctcagaccg tcgaaggtct cacactcttc ttgattctcc
    31201 ggtcattcgc cgctggctgc tcagccgtaa ccggcatgga agtgatttcc aatggcgtca
    31261 aagcctttag gccgcccgag tcgcacaatg ccgcaatcac catggtctgg atgtcggtta
    31321 ttctcgccgt cctcttcata ggcataagct ggatggccta tcactatgga atcttggcca
    31381 aagaagatga aacggtcgtc tcacaattgg cccggctgac cttcggcacc ggcacaattt
    31441 attatgcaat ccaaatcggc acgatgatgc tcttggtcct cgcagccaat agcgcctttg
    31501 ccgggtttcc tcacctctcg tctatcctcg cacgtgatgg gttcatgcca catcagatgg
    31561 cgacgtttgg cgatcggctc gtcttctcca atggcgtgat cattctcggg ttttttgcct
    31621 gcctgctcct ggtcatcttt cacggtaata cgcacgcctt gattcccct
//
LOCUS       DATIFR010000025         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2503551, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000025 DATIFR010000000
VERSION     DATIFR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503551"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VLD60_01305"
     CDS             complement(<1..762)
                     /locus_tag="VLD60_01305"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HSB43615.1"
                     /translation="MAQSPLTIKQFLVENQPASSDKGNDLSGLFAAIGLIGKSLARDL
                     RIAGLLNILGTTGETNVQGEAVKKLDEIANETFVRILQQGGVACALASEEMEKPVMIS
                     RQGRRSPHMVLFDPLDGSSNTDVNMPLGSIFSIVAAPKSEESPVENDLLQKGTEQVAA
                     GYFLFGPSTMLVFTTGQGVHGFTLDPSLGEYLLSHEAMRIPARGKVYAINEGNYHKWP
                     AGTRQYIDSLKKQDKSTGRPYSGRYSGCLVADVHRI"
     gene            complement(903..1682)
                     /locus_tag="VLD60_01310"
     CDS             complement(903..1682)
                     /locus_tag="VLD60_01310"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HSB43616.1"
                     /translation="MKLLIGFLAATTAVVLSGQCVAAQVADPAPNRSVQVGERLTYEI
                     SWLNIMAGTAVMEVGEGGSSGESNNARLITTAQSRPAITKFFPVDNRVESEFDLRALL
                     PQHMTFRRREGRKKEDIEYTFHHREGTVTATRGGTTETLPILPGTQDLISCLYYVRSI
                     LPMKPGASLTMNVHHDKKNRTIEVRVEDIETVEGVWGKIDAARVLVIMPFQGIFLNQG
                     NIRVWFTTDERRTPVRMKAKVIIGSIVADLVNGFSPAGASR"
     gene            complement(1722..>2725)
                     /locus_tag="VLD60_01315"
     CDS             complement(1722..>2725)
                     /locus_tag="VLD60_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSB43617.1"
                     /translation="VSGQGKLSEHPIIPDYLAYIKESFAGLDAKRLHVVIDCGNGVAA
                     LVAKEALELLGCRVTGLYCELDGRFPNHHPDPTVLENLDDLVRAVTQHKADVGIGYDG
                     DADRIGAIDERGEVLWGDRLMVVYSRDILAVKPGSTIISEVKASQCLYDDIAKHGGKP
                     IMWKTGHSLIKAKMKSEKAVLAGEMSGHMFFADRYFGYDDAIYASCRLVEILAKTGKS
                     LSSLVADLPVTSVTPEIRVDTPDAIKFDLVKKVQARLTSYVQSRQPIGPQKFVVRDVV
                     TIDGVRAIFDDGWGLIRASNTQPALVLRFEATSPDRLAAIRAVIEGELAEVKRLLGP"
BASE COUNT          625 a          802 c          711 g          587 t
ORIGIN      
        1 aatccggtgg acatcagcaa caagacaacc ggagtaccgg ccgctatagg gacgccctgt
       61 cgatttatcc tgtttcttga gggagtcgat gtactgccgc gtcccggccg gccacttatg
      121 gtagttcccc tcgttgatcg cgtagacctt cccgcgggcg ggaatcctca tagcctcatg
      181 ggacagcagg tactcgccga gactcggatc cagcgtaaag ccgtgaaccc cctggccggt
      241 cgtgaagacc agcatcgtgc tcgggccgaa taggaagtat cctgccgcca cctgctcggt
      301 ccccttctgt aagaggtcgt tctcaaccgg cgattcttcg gactttggtg ccgcaacgat
      361 cgaaaaaatc gaaccaagcg gcatgtttac atcggtattg gatgagccgt ccaacggatc
      421 gaacagaacc atatggggag atcgccgtcc ttggcgggag atcatcacgg gcttttccat
      481 ctcttcagag gccagcgcgc aggcaacgcc gccttgctgt aagatcctca caaacgtctc
      541 gttggctatc tcatcgagct ttttgaccgc ttcaccctgt acgttggttt cccccgtcgt
      601 tcccaagatg ttgaggaggc cggcgattcg aagatctcgg gcaagcgact ttccgatcag
      661 accgatcgcg gcgaaaagac ccgagaggtc attccccttg tcagacgaag ctggttggtt
      721 ttcaacgagg aattgtttga tcgtgagcgg agactgtgcc atgaacggga aggaccttta
      781 ttaatccaaa aagactcccg gggcctggtg aaacttcaag cccggcgagc tccgaataat
      841 gggataattg tggctaatta tagccggtct cactggggtg ggcaatgaat cccccaaatc
      901 ccttatctgg aggcccccgc aggggaaaat ccgttgacca ggtcggccac aatagacccg
      961 ataatcacct tagccttcat gcgaactgga gtccgccgct catcggtggt aaaccacact
     1021 cgaatgttgc cttgattgag aaagatgccc tgaaacggca tgatcaccag caccctcgca
     1081 gcatcgattt tcccccatac tccctcgacg gtctcgatgt cctccacgcg gacttcaatc
     1141 gtacgatttt tcttatcgtg atggacattc atcgtcaacg acgcccccgg cttcatgggc
     1201 aatatgctgc gcacataata gagacaagag ataaggtcct gggtgccggg tagaatcggg
     1261 agggtttcgg ttgttccacc tctggtcgcg gtcacggtcc cctcacgatg gtggaacgtg
     1321 tactcgatat cctccttctt ccttccctca cgccgtcgaa aggtcatgtg ttgcgggagc
     1381 aatgcgcgga ggtcgaattc ggactctaca cgattatcga cagggaagaa cttggtgatc
     1441 gcgggtcgag attgagccgt ggtgatcagc ctggcattat tggattctcc tgaactgcct
     1501 ccctccccca cttccatcac ggctgttccc gccatgatat tcaaccagga gatttcatac
     1561 gtcagtctct caccaacttg gacggatcga ttgggagcag gatcggcaac ctgcgccgcg
     1621 acacactggc cactcagaac aacggcagtg gtcgcggcaa gaaaccctat gagcaacttc
     1681 atggtggaag cggaccttcc ggatcggcag ggaggaaatg gctagggacc taagaggcgt
     1741 ttgacctcgg caagttctcc ttcaataacg gctctgatgg cagcaagccg gtcaggggac
     1801 gtggcttcga accgcaagac caatgccggc tgggtattgg aagcacggat cagcccccac
     1861 ccgtcatcga aaatggcgcg aaccccgtcg atggtgacca cgtcgcgcac gacaaacttc
     1921 tggggaccaa tcggctgccg agattgcaca taggaagtca gacgtgcctg gaccttcttc
     1981 accaaatcaa acttgatcgc atctggcgta tcaacccgaa tctccggcgt cacagaggtg
     2041 acgggaagat cggccaccaa cgacgagagc gatttaccag tctttgcaag aatctcgacc
     2101 aaccggcagg acgcgtaaat cgcgtcgtca tacccgaaat agcgatcggc aaaaaacata
     2161 tgcccggaca tctcgccagc caaaaccgcc ttttcactct tcattttagc cttaatcagt
     2221 gaatggccgg ttttccacat gatcggcttg ccgccatgct tggcgatgtc gtcatacaga
     2281 cattgggagg ctttcacctc ggagataatc gtgctgccag gcttcacggc caggatatcg
     2341 cgggaataca ccaccatgag ccggtctccc cataacacct caccccgctc atctattgca
     2401 ccgatcctgt ccgcatcgcc gtcatagccg atcccaacat cggctttatg ttgtgtgacc
     2461 gcgcgaacca aatcatccaa attctcaagg accgtcgggt caggatgatg gttcgggaat
     2521 cgtccatcca gctcgcaata tagcccggtt acgcggcagc ccaacaactc caaggcctct
     2581 ttcgccacca atgctgccac tccgttgccg caatcgatga ccacatgcaa acgcttggca
     2641 tccagcccag caaagctctc cttgatgtag gcaagatagt cgggaatgat cgggtgctca
     2701 gacagtttcc cctggccgga cacaa
//
LOCUS       DATIFR010000026         5060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_971811, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000026 DATIFR010000000
VERSION     DATIFR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971811"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VLD60_01320"
     CDS             complement(<1..471)
                     /locus_tag="VLD60_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSB43618.1"
                     /translation="MFKAFRKQEGFTLIELMIVVAIIGILAAIAIPNFVAYQAKSRQS
                     EAKINLGAIFTSATAFAAEQATPTYVPGPTLDPIGWSPSGQPRYTYFYATGAVGAASA
                     SAATAFTLPSSVVAPTSCTSAPTTGGNAVAASATVGTTPAGFTAGAQGNVDSDAT"
     gene            complement(853..2682)
                     /locus_tag="VLD60_01325"
     CDS             complement(853..2682)
                     /locus_tag="VLD60_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB43619.1"
                     /translation="MGYLPSNTFQFPELGNGLLAALIPLVIMACAAAPLHQETATPVT
                     AQTKQPAAPTPDSSATYHFMLGYQAELAQDNETALREYRAALKADPASREVKARLAGL
                     YFGLGDLTNAVQYAEEVGEGTGQDAQMLTQMAGILASAGKPDRALRLLDLAIDRAPER
                     GESYFPKGIILMNQKRLPEAEQAVKQGLKYAPDSPIGYYYLGRISIEAGNAEQALDSF
                     NRAIAANPSFEPAYLAQASFYESRQEKDKAIAVLQKYLQNVNPRNKDIRQHLIQLYVS
                     TKDYAGGLAELEKLLEEDPGDLDAQLRKALIYGEKKEYSKAIDQLTSILKARPAELKV
                     RDYLGYLYEETKEFPKAIEAYQFNIQLDPTFVESHIHLGVLQYRLKKFPEAITHLSEA
                     VRLAPKQPEPHIVLGLAYLQSEQFEKASQAFEEGIRHNPKSADLHFNLGTAYDKLNRF
                     EDVVRAMETALSLDPHHADAMNYLGYSYAERGIKIDQAVSLTRQAVALKPDNGYYVDS
                     LGWAFYKSGLLAEALTEIKRAVALVGDDPVIYEHLGEIYMKQEKLSDAREAWLHSLEL
                     DPSNEKLLQRFRDQGMGDPSQEDRIQQAKRRVSEKIQTQQVTP"
     gene            complement(2783..4135)
                     /gene="dnaB"
                     /locus_tag="VLD60_01330"
     CDS             complement(2783..4135)
                     /gene="dnaB"
                     /locus_tag="VLD60_01330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSB43620.1"
                     /translation="MKSLTAVDLSQPKLPPQNLEAEQSVLGAILLDNAAMPKAMELLV
                     EEDFYRTAHKKVYRAMLDLSDIGEVIDQITLTERLKGQGELESIGGAAYLAELVQIVP
                     SSANIRYHCKIVRDKAVARQLINTSTEVLARGYEGTASIDDLLDFAERSVFSIAQGKL
                     ERSFSPINQIIKESLDLVDKLSKRKEHVTGVPTGYYDLDDITAGLQPSDLVVVAGRPS
                     MGKTSLALGFATHAAIHANSVVGIFSLEMSKPQIVLRMLSSEARVDSHALRTGKLQKE
                     DWWRLAEAAGRLEQAPIYIDDTGGITVQQMRGKARRLKAEKGLDLLIVDYLQLMQGRS
                     DSESRQQEISDISRSLKALAKELNVPVVALSQLSRAVEARKPPIPMLADLRESGAIEQ
                     DADVVMFIYREDVYDQNSERKGIADIIVSKHRNGPIGKKELFFQDRFAKFESLDLRES
                     "
     gene            complement(4132..>5060)
                     /locus_tag="VLD60_01335"
     CDS             complement(4132..>5060)
                     /locus_tag="VLD60_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSB43621.1"
                     /translation="ALLIECLQAVGLRSFKISLGHVGFFKGLLVGAGLSPEGQKRAEQ
                     AAARKDLPKLEEILSQERVTRAFFRTILEAPELCGQAEVLVRGREVAGDEKPLVQPLD
                     RLTKVYQLLCSAGYQDLLFLDLGEFRGFDYYDGVVFDVFADGVGVELGGGGRYDHLIG
                     RFGRNIPSTGFALNVDRLFRGLNLSGKTSEAARVDVLVAAPRTAAKQLISVARQLRQS
                     GKRVVQQVLAASGNDLISAAGEAGLGAQADTVVVVGADGTKGEQVLVIQQTAQQSAMR
                     GNGRPRMKKKLMSITDLVVQMRRARTGTGRAR"
BASE COUNT         1054 a         1502 c         1336 g         1168 t
ORIGIN      
        1 ggtggcatcg gagtcgacgt tcccctgcgc acctgccgta aacccagccg gtgtcgttcc
       61 taccgttgca cttgcggcta ctgcattacc tccagtcgtc ggtgccgatg tacagctagt
      121 tggtgctact acgctcgaag gaagggtaaa cgccgtggcc gcggatgctg aagcagcacc
      181 gaccgcgccc gtggcataga agtacgtata gcgtggttgt ccacttggcg accagccgat
      241 tggatcgagc gtggggcctg gaacatacgt cggagttgcc tgttcagccg caaatgcggt
      301 ggccgaagta aagatagcgc caaggttgat cttggcctcc gactgccgcg atttcgcctg
      361 gtaggcaacg aagttgggaa tggcgatggc cgccaggatc ccgatgatcg ccacgacgat
      421 catcaactcg atgagggtga aaccctcttg tttccggaat gccttaaaca tcgtactgcc
      481 tccttgttga tggttcactg acggcccaag ctgctctcta tgctcttgcc ttggtcacct
      541 gcggcgctcg cgttgccgcc tacctgcccc tctagcaggt ttggtgcctg tcagtgatgt
      601 cacaaaccct atcatcgacc ctttgaaaag caaggagtta tgaaaactcg acaaagaagc
      661 atacctgttg ggcgtcgctt ctgtactccg cattctgctc gggattgccg agaattggca
      721 ctttttcact cagcccgtac tcaaatcgtg cacaggacgg acctcaggct caacaccgaa
      781 tacctggaaa tcgaagattt tcgcctgttg agggaacaac cagcggctgg atatgagccc
      841 tgaatggaaa gcctacgggg taacctgctg agtttgtatc ttctctgata cgcggcgctt
      901 ggcctgctgg atacggtctt cctgagacgg atcgcccata ccttggtcgc ggaatcgctg
      961 gagcagtttt tcgttggagg gatcgagctc aagggaatga agccaggctt cccgggcatc
     1021 cgatagcttt tcctgcttca tataaatctc gcccaagtgt tcgtaaatca cggggtcgtc
     1081 accgaccaac gccaccgccc gcttgatctc cgtaagcgcc tctgcaagta accctgattt
     1141 gtagaacgcc cacccgaggc tatcgacgta atacccattg tcaggcttga gcgcgaccgc
     1201 ctgtctggtg agggacacag cctggtctat cttgatcccc cgctccgcgt agctataccc
     1261 gaggtagttc atggcgtcgg catggtgcgg gtctaaggat agcgccgttt ccatggcacg
     1321 caccacatcc tcaaatcggt tgagcttgtc atacgccgtg cccaggttga aatgcaggtc
     1381 ggcactcttc gggttgtgac gaatcccctc ctcaaatgcc tgcgatgcct tttcaaactg
     1441 ttcagattga aggtaggcaa gtccgagcac gatgtgaggt tcgggttgct tcggagcgag
     1501 acgcaccgcc tcgctcaggt gcgtaatggc ttcgggaaat ttcttgagac gatactgcag
     1561 gactccaaga tggatatgac tttccacaaa ggtcggatct aattggatgt tgaactgata
     1621 ggcctcaatg gccttcggaa actccttcgt ctcttcatac aaatagccca gatagtcccg
     1681 gaccttgagt tcagcaggcc tggccttcag aatagacgtg agttgatcga tcgccttgga
     1741 atactccttc ttctcgccgt agatcagggc ctttcgcagt tgtgcatcaa gatctccggg
     1801 atcctcttcc aacagcttct ccagctcggc cagtccccct gcatagtcct tcgtcgatac
     1861 atacaactga atgagatgct ggcgaatgtc tttattccgg gggttcacgt tctggaggta
     1921 cttttggaga acggcgatgg ccttgtcctt ctcctggcgg gattcataga acgaggcctg
     1981 ggcaagatag gccggctcaa aagaaggatt cgccgcgatg gcgcgattga aactgtccaa
     2041 tgcctgctcg gcattaccgg cttctatgga gatgcggccc aagtagtagt agccgatcgg
     2101 ggagtccggt gcgtatttca acccttgttt gacggcttgt tccgcttcag gcagccgctt
     2161 ttgattcatt aaaataatgc ctttggggaa gtaggactct cccctttcag gagcacggtc
     2221 gatagccaga tccaatagcc gaagcgcgcg gtcgggcttc cccgcactgg ccagaatgcc
     2281 ggccatttgc gtcagcattt gcgcgtcttg acctgtcccc tctccgacct cctccgcata
     2341 ctgcaccgcg ttggtcagat cgcctaagcc aaaatacagc ccggctaacc gggctttgac
     2401 ctcgcgtgag gcggggtcag ccttgagcgc cgcgcgatac tctctcaacg ctgtttcgtt
     2461 atcctgggca agttcagctt gataccccag catgaagtga taggtggctg aagaatcggg
     2521 agtgggtgcc gcaggttgct tcgtttgtgc cgtaacaggg gttgccgttt cctgatgaag
     2581 cggcgcagcc gcacaggcca tgatgacgag ggggattagt gcggcgagta gcccgttccc
     2641 caactcggga aactgaaacg tattggacgg tagataaccc acggtcgacg gttttccctg
     2701 cactaaaggc tttactaaaa ttgcttggag gtgaggcacg tgagagaatt ataccgaccg
     2761 taggagcgat gcgcaaacca cttcacgatt ctctaagatc gagactttca aacttggcaa
     2821 agcgatcttg gaaaaagagc tctttttttc cgatgggccc gttccgatgc ttgctgacga
     2881 tgatgtcggc gatgcccttc cgttccgaat tttgatcgta gacatcttct cgataaataa
     2941 acatgaccac gtcggcatcc tgttcgattg ctccgctctc gcgcaggtcg gccagcatcg
     3001 ggatcggggg ctttcgggct tccaccgccc ggctcaactg ggacagggcc acaaccggca
     3061 cgttcagctc cttggccaag gctttcaacg atctggagat atcggagatt tcctgctggc
     3121 gagattctga gtcacttcgc ccttgcatca gctgaagata atccacaatc agcagatcga
     3181 gccccttctc agccttgaga cgacgggcct tcccgcgcat ctgctgcacc gtgatcccgc
     3241 cggtgtcatc gatgtagatt ggcgcctgct ctaatcgccc ggccgcctcc gccaatcgcc
     3301 accaatcctc tttctgcaac ttacccgtcc gcaaagcatg cgaatcgacc cgcgcttccg
     3361 aactcaacat acgcagcacg atctgaggtt tggacatttc caggctgaag atgccgacta
     3421 cggagttcgc atgaatagcc gcgtgcgttg caaaccccag cgcgagactc gtcttcccca
     3481 tactcggccg gccagccacc accaccaggt ccgacggctg aagtcctgcc gtaatgtcgt
     3541 cgagatcgta atagccggtg ggcactcccg tgacatgctc cttccgtttc gagagcttgt
     3601 cgaccagatc aaggctttct ttgatgatct gattgatcgg gctgaaggac cgctccaact
     3661 tgccttgagc gatgctgaag acagaccgct ccgcgaaatc gagcagatca tcgatcgacg
     3721 ccgtgccttc atagcccctg gccaacacct ccgtcgacgt gttgatcaat tgtcgcgcaa
     3781 ccgctttgtc acgaacaatc ttgcagtgat atctgatatt ggctgaactg gggacgatct
     3841 gaaccaattc ggcgaggtag gctgcgcctc caatcgattc cagttctccc tgtcccttca
     3901 accgctcagt caaggtgatc tgatcgatca cttcgccgat gtccgacaaa tcgagcatcg
     3961 ctcgatacac cttcttatgc gccgtccggt agaagtcctc ctcgaccaga agttccatcg
     4021 ctttcggcat cgcagcgtta tccaagagga tggcgcccag caccgattgc tctgcctcca
     4081 gattttgagg gggcagcttc ggctgcgaga gatcgacggc cgtgagcgat ttcatcgtgc
     4141 tcttcctgtt cccgtacgag ctcgtcgcat ttgcacaacg agatcggtga tcgacatcag
     4201 tttcttcttc attcgaggac ggccattccc gcgcatcgca ctttgctggg ccgtctgttg
     4261 aatcactagc acctgttcgc ccttggtccc atcagcaccg actactacca cggtatcggc
     4321 ctgggcccca aggccggctt ctcccgccgc gctgattaag tcgttccccg atgccgcgag
     4381 aacctgctgc accactcgct tccccgattg tcgaagttgt cgggcaacag agatgagttg
     4441 cttcgctgct gtgcgcggcg ccgctacgag gacatccacg cgagccgctt ccgacgtctt
     4501 gccggagagg ttaagccccc gaaagagtcg atccacattc agggcaaacc cggttgatgg
     4561 gatgttccga ccaaatcgac cgattaagtg gtcatagcgc ccgccgccgc ccaattcgac
     4621 gccaactcca tcggcaaaga cgtcaaacac gacaccatca tagtagtcga atcccctgaa
     4681 ctcgcccaaa tcgagaaaca ataagtcttg gtatccggcc gaacagagca gctgatagac
     4741 cttggtcaac cgatccagtg gctgtaccag gggcttttcg tcgccggcca cttcccgtcc
     4801 cctcacgagc acttcagcct gaccacagag ctccggcgct tccagaatcg tgcggaaaaa
     4861 cgctcttgtg acccgctcct gcgagagaat ttcttcgagc ttcggcaaat cctttctggc
     4921 cgcagcctgt tcagcccgct tctgcccctc cggcgacaat cccgctccca cgagcaaacc
     4981 cttgaaaaat ccaacgtgtc caagcgagat tttgaatgag cgtaacccaa ctgcctgcag
     5041 gcactcgata agaagcgcga
//
LOCUS       DATIFR010000027         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2313789, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000027 DATIFR010000000
VERSION     DATIFR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313789"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1309
                     /locus_tag="VLD60_01340"
     CDS             53..1309
                     /locus_tag="VLD60_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43622.1"
                     /translation="MKPGEVHSAIITGLVIAFGLLSHSPLLWAASPPAAGQQPATAAS
                     APADDREDQIVANYLRDCSGKGVKDASCDKQRNDFVAILKEDLLTLGSTADRKYIPDI
                     VRLFRKRPEVELRIDAAHALGMIGPEDSDVKMLVPMTNDPVPDVRYAVFNMIPRGKGK
                     ALDLLKERIARQNPGRELEKPADPAKFSMPVAPDSVYLFESSDATKGRLSYLAKGKTE
                     PAQFFKTKAKKGPYKWDQFKEQYRYQLQDEEEALNQAQQAAGKQLGNEKPPDPATNME
                     AYVEYMQKLGSVSTQGSMGRMFFDTYQANLYGTPTVYILEERQIGQRSYPTRYVVVYQ
                     ELALKKPGYRLSWTTVPDDALKAAQVASLKEQREEEVLKAASKKEEEAAKKREAELDA
                     LTKKKDAAEKKQFKKGQEDLEKALGF"
     gene            1336..1827
                     /locus_tag="VLD60_01345"
     CDS             1336..1827
                     /locus_tag="VLD60_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43623.1"
                     /translation="MFNTFKAMLAVTAAFALALLAPAVAGAQNISDRDTNEIAGYVLT
                     DAGLSKYTQAVRKLQPLTGQLPQDCDGEEGPKSLNDAAARMDGIPQVKSALKAAGMTS
                     REYLVFSWSVFQNGLAAWALEQPGGKLPPGVKMANVNFYRAHEAELKKLGELAKHADC
                     DNT"
     gene            1889..2179
                     /locus_tag="VLD60_01350"
     CDS             1889..2179
                     /locus_tag="VLD60_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43624.1"
                     /translation="MKKREAEKKKFKKGQADLEKELGFYERSARVQRQASAAGTSVGD
                     GRMAVSRSQEGNSLTAIGLIATTALLGQHPLTRAGVIRDARRARVQRIFYRS"
BASE COUNT          611 a          646 c          733 g          435 t
ORIGIN      
        1 cgagatagca cagctcttgt agctggacga gtagattgta aggaggatac gtatgaagcc
       61 aggtgaagtc cattccgcca tcatcacagg cttagtcata gcatttgggc tcctctctca
      121 ctctcctctg ctttgggcag catcgccacc ggctgcgggc cagcaaccag cgacggccgc
      181 ttcggcacca gccgatgacc gagaagatca gatagtggcg aactatctgc gggactgcag
      241 tggcaaggga gtgaaagacg cgagttgtga caaacaaaga aacgatttcg tcgcgatctt
      301 gaaggaggac ttgttgacgt taggttctac cgctgatcgc aagtatatcc ctgatattgt
      361 tcgattgttt cgaaaaagac ctgaagtcga attgcggatt gacgcggcac atgcgctcgg
      421 catgatcggc ccggaagata gcgacgtgaa gatgctggtg ccgatgacca atgatccggt
      481 tccggacgtc cgatatgcgg tattcaacat gattcctcgc gggaaaggaa aggccctgga
      541 tttattgaag gagcgcatcg cgcgccagaa tccgggccgg gagcttgaaa agccagccga
      601 tccggccaag ttctcgatgc cggttgcgcc ggacagcgtc tatctttttg agtccagcga
      661 tgcgacaaag ggccgtctgt cgtatctggc gaaaggcaag accgagccag ctcagttttt
      721 taagaccaag gcgaaaaaag gtccttataa gtgggaccag ttcaaagagc agtatcgcta
      781 tcagttgcaa gatgaagagg aagcactcaa ccaggcacaa caggcggcgg gcaagcagct
      841 ggggaacgag aagcctccgg atccggccac gaacatggag gcgtacgtgg aatacatgca
      901 aaaattggga tcagtgtcga cgcaaggaag catgggacgg atgttcttcg atacctatca
      961 ggccaatctc tatggcacgc cgaccgtcta tatcttggaa gaacgacaaa tcggccagcg
     1021 cagctatccc acccgctacg tcgtggtcta tcaggaactg gctttgaaaa agccgggcta
     1081 ccgcttgtcg tggacgacgg tgcccgacga cgcactcaag gcagcgcaag ttgcgtcgct
     1141 caaggagcag cgagaggaag aggttctgaa agccgccagc aagaaggaag aggaggccgc
     1201 gaaaaagcgt gaagcggagc tggatgccct cacaaagaaa aaggatgcgg cggaaaagaa
     1261 gcagttcaag aagggccagg aagatctgga aaaggccctc ggtttttgag cggaatcgag
     1321 ccccggagga tcgtcatgtt caatacgttc aaggccatgc tcgcggtaac agccgccttc
     1381 gccctcgcgc tgctggcgcc ggcagttgca ggagcccaga atatttccga ccgcgacacc
     1441 aacgagatcg ccggctacgt gctgaccgac gcggggttgt cgaagtacac acaagccgtg
     1501 cgcaagctgc aaccgctgac ggggcaattg ccgcaagatt gcgatggcga ggagggcccg
     1561 aagtctctca atgacgcggc ggcgcggatg gacggaatcc cccaagtgaa gtcggcgctc
     1621 aaggccgccg gcatgacgtc gcgcgagtac ctcgtgttct cctggtcggt gtttcaaaac
     1681 ggcttggccg cttgggcgct tgagcagcct ggaggcaagt tgccgcccgg tgtgaagatg
     1741 gcgaatgtca atttctatcg cgcgcatgaa gccgaactga agaaacttgg cgagctagcc
     1801 aaacacgcgg attgtgacaa tacatgatcg taccggcgaa tgggcagagg ggatggaaga
     1861 agtagctcgt aacaacgagg acgaggcctt gaaaaaacgt gaagcggaga aaaagaaatt
     1921 caagaagggc caggccgatc tcgaaaaaga gctgggattc tacgagcgct ctgcccgggt
     1981 tcagcgtcag gcttctgcgg ctggaacgag cgtgggagac ggccgaatgg ccgtctcccg
     2041 atcgcaagaa gggaacagtc taacggcgat tggcttgatc gcgacgacag ctctcctcgg
     2101 ccagcatccg ctgacccgcg ctggcgtcat tcgggatgcg cgccgtgcac gcgttcaaag
     2161 aatcttctac agatcctgag gcgatccggt cgatattctt cgtcgggccg gttgccgtcg
     2221 tcccaccacc ggaggccgag ccggtatcgg acgaacccat cgtcccctcg ctcgcacaac
     2281 cgcccgccat aagactcacc aaggcaacaa cacctaaagc atgcatcgat cgcattctca
     2341 tgacccaagt cctccctacg agatgaatgt gaatacaacc cggcctgggc gatcctacat
     2401 gcgaatcgag ggaaagaaca atccc
//
LOCUS       DATIFR010000028         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2314436, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000028 DATIFR010000000
VERSION     DATIFR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314436"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VLD60_01355"
     CDS             <1..602
                     /locus_tag="VLD60_01355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB43625.1"
                     /translation="MLCTWARDHGVIGIGIDMSQLFTEQAKLRAEELGVAHQVKFIHG
                     DAVGYVSDEKASVAACVGATWIAGGVVGTIELLARSLRTGGIILIGEPYWRQLPPTED
                     VAKGCLANSISDFLMLPELLASFGHLGYDVVEMVLADQDGWDRYEAAKWLTMRRWLEA
                     NPDDEFAKDVRAKLTSEPVRYAAYTREYLGWGVFALMPR"
     gene            602..811
                     /locus_tag="VLD60_01360"
     CDS             602..811
                     /locus_tag="VLD60_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43626.1"
                     /translation="MRWRIRRIVNGCAARQLVARRDVIAPEYIFYGLVIHTVNRKVYL
                     KQAQQFIQADAASRRGLIQALACMM"
     gene            complement(1092..1439)
                     /locus_tag="VLD60_01365"
     CDS             complement(1092..1439)
                     /locus_tag="VLD60_01365"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB43627.1"
                     /translation="MRKIEYPFTIRRLSKAEGGGWLVEFPDLPGCIADGETIEEAVEN
                     ASDSLRSWLATAKEFKDPVPEPSAGLSGKWVQRVPKSLHAKLAAKAEKEGVSLNTLVV
                     SLIAEGLGRRAAR"
     gene            complement(1426..1668)
                     /locus_tag="VLD60_01370"
     CDS             complement(1426..1668)
                     /locus_tag="VLD60_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43628.1"
                     /translation="MTAAAKILGRMRNNPTGWRIEDLKTVADRFNIEYRQPGTSHVTF
                     RHPSGAKLTVPARRPIKPVYVKKFLELIDEVAHEKD"
     gene            2070..2624
                     /locus_tag="VLD60_01375"
     CDS             2070..2624
                     /locus_tag="VLD60_01375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSB43629.1"
                     /translation="MNQYRIAVVVGSLRRDSFNRKLAKALVKLAPSDFSFEFLNIDDL
                     PLYNQDDDANQAASVKRLKGEITAAKGLLFVTPEYNRSIPGVLKNSIDNASRPYGQNA
                     WAGKPAGVLGASIGQIGSALAQQHLRNILAYLDVPTLGQPEVFLHVKDGFFDEAGDIT
                     NTGTIKFLQEWMNRYVAWVKMHTA"
BASE COUNT          566 a          732 c          751 g          588 t
ORIGIN      
        1 agatgctgtg cacctgggca cgcgatcacg gcgtcatcgg catcggcatc gacatgagcc
       61 agttgttcac cgagcaagcg aaactccgtg ctgaagaact cggcgtcgcc catcaagtca
      121 agttcatcca tggcgatgct gtcggctatg tctctgacga gaaggccagt gtggcagcct
      181 gtgtcggtgc cacctggatc gccgggggag tcgtcggcac tatcgagcta ctggcgcgga
      241 gcctgcgcac cggagggatc atcctcatcg gcgagccgta ctggcggcag ttaccgccga
      301 cggaagatgt tgccaagggg tgtcttgcca actcaatctc cgattttctc atgcttccgg
      361 aacttctcgc gtctttcggc caccttggct acgacgtcgt tgaaatggtt ctggctgacc
      421 aagacggctg ggacagatac gaggcggcca aatggcttac catgcgccga tggcttgaag
      481 ccaatcccga cgacgagttc gcgaaagatg ttcgagccaa actgacctcg gaacctgtgc
      541 gctacgccgc atacacgcgt gaatacctgg gctggggtgt gttcgcgctg atgccgcggt
      601 gatgcgttgg cgcatccggc ggatcgtcaa cggctgcgcc gcgcgacagc tggtggctcg
      661 ccgagatgtg attgcgcctg aatacatttt ttatggtcta gttatccata cagtcaacag
      721 aaaggtctat ctcaagcagg cccaacaatt tattcaagcc gacgctgctt cgcggcgtgg
      781 attaattcag gcgttagctt gcatgatgtg agaaaggata atgacgaaga aacttccgat
      841 aggcgcattt ttcgatatta aaaaagttga gcatcgcctt tcgactatgc accgggatgt
      901 tcaacttctt cgtgataatg cggttaaccg gcctgcaatt ttgactcgga tcggcgtcta
      961 accttgaccc atgtgtaatt cgaaagatat tcacagacaa ctggatctta cggtccgaaa
     1021 gagtgggtca gagttggatg ccgaaaacgc cccaagacgg gtcatttttc aaagccgatc
     1081 tacatacgca gctaccgcgc agcccggcgg ccgagccctt cggcgatgag tgagacgacc
     1141 agcgtgttca agctcactcc ctccttctcg gccttggcgg caagcttggc gtgaaggctc
     1201 ttcggaaccc gctgaaccca tttcccgctc aatcccgccg agggttcggg gaccgggtcc
     1261 ttgaactcct ttgcggtggc cagccaggag cgaagcgagt ccgaggcgtt ctccaccgcc
     1321 tcctcgatcg tctctccgtc ggcgatgcac ccggggaggt cggggaactc caccagccat
     1381 ccgccacctt ccgccttcga cagacgacgg atcgtgaagg gatactcaat ctttctcatg
     1441 ggccacctca tcgatcagtt cgaggaattt cttcacatac accggcttga tcggtcttcg
     1501 ggccgggaca gtaagctttg ctccagaggg atgtcggaac gtgacgtgac ttgtccccgg
     1561 ctgccggtac tcgatgttga atcggtccgc gacggttttc aggtcctcga tcctccaacc
     1621 cgtcgggttg ttccgcattc ttccgaggat cttggctgcc gccgtcatga agccatagta
     1681 tcaatggcga tactacaaat caagcggggc cgtgtggaag ttttgtggaa gttgggagaa
     1741 aaaaggggga tattttctat aaaaactgat aaatttggcg agttggccta tttgtaagtc
     1801 gttgatatca tagccacatt tagggcatta tagggtgtgg gcttgcttga ggggatggtg
     1861 gggaaacccg tgggggttcg agtcccccct tcggcaccat cgcaaaatta aaagggaact
     1921 tggctttgcg ccaggttccc ttttttcgta aaatcggcaa atgtggctca aatggggctc
     1981 gcgggcacca agcatcggga tgaaattggg gtatcatcgc aagggaccgt tccggggcgc
     2041 ctaattaacc gatagggagg tcaagcgtca tgaaccaata ccgtatcgcc gtggtcgtcg
     2101 gcagcctgcg tcgcgattcg ttcaatcgga aacttgcaaa ggccctcgtc aaactggcgc
     2161 cgtccgactt ctccttcgag ttcctgaata tcgacgattt gccgctctac aatcaggacg
     2221 acgatgccaa ccaggccgca tccgtgaaac ggctgaaagg agagatcacg gcggccaagg
     2281 gccttctctt cgtcactccc gaatacaacc gctcgatccc cggcgtgcta aagaactcga
     2341 tcgacaacgc ttcccgccct tacggtcaaa atgcctgggc gggcaagccc gccggtgttt
     2401 tgggcgcatc catcggccag atcggctcgg ccttggcaca acagcacctg cgaaacatcc
     2461 tcgcctacct ggatgtgccg acgcttggcc agccggaagt gttcctccac gtcaaggacg
     2521 ggtttttcga tgaggccggc gatatcacca acacgggtac gataaaattc cttcaggaat
     2581 ggatgaaccg ctacgtcgcg tgggtaaaga tgcacacggc ttgatcggaa gcttccg
//
LOCUS       DATIFR010000029        29237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1740470, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000029 DATIFR010000000
VERSION     DATIFR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 29237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29237
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740470"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VLD60_01380"
     CDS             complement(<1..337)
                     /locus_tag="VLD60_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSB43630.1"
                     /translation="MKVPLGKGIFLIAAPNLRDPNFRQAVVLLCEHGAEGALGVVVNR
                     PTAMSISEALPQVPILEGQRHVLFSGGPVQPNQVMLLYRLNQLPENSHHVFDGVCLGG
                     DMELVDKILT"
     gene            complement(544..1332)
                     /locus_tag="VLD60_01385"
     CDS             complement(544..1332)
                     /locus_tag="VLD60_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSB43631.1"
                     /translation="MRVGYYQNNPEFGKVTENLDRISARLDEAAADLIVLPELCASGY
                     QFVSRDEVRALAEPIPDGPTTRRLLDIAKRKHLIIVAGLPERAGASLYNSAVAVGPQG
                     VIGSYRKTHLFFEETLFFTPGDTGFQVWDIAQAKIGVMICFDWYYPEAARTLALKGAE
                     IICHPSNLVLPNCPDSMPVRCLENRVFAITCNRTGSEARGGKDPLTFIGNSEIVTPRG
                     TILHRGPRDQEDLYIVEIDPAEARNKSVTRYNDLLRDRRESLYR"
     gene            complement(1437..1778)
                     /gene="smbP"
                     /locus_tag="VLD60_01390"
     CDS             complement(1437..1778)
                     /gene="smbP"
                     /locus_tag="VLD60_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HSB43632.1"
                     /translation="MTMRIQRGVLMLATVALLGMPLVAQADNKHVSEAIEHAKEAAAH
                     GKQGHADALVKHAEGALHHAEAAQKDVKNPHLDEGVKELKAAVEHGKAGHADVATKHA
                     EGAVTHLSEVK"
     gene            complement(1871..2749)
                     /locus_tag="VLD60_01395"
     CDS             complement(1871..2749)
                     /locus_tag="VLD60_01395"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSB43633.1"
                     /translation="MTHPLLIDTESLQSQLGQPGLVIIDVRGKAAYEFGGHIPGAVHT
                     TWHDYSDPNAVAKGLIDPDLKRMEQKMRALGINADSQVVIYSNPFDNWGDEGRMYWML
                     EYLGHTNLRILDGGWVKWVEERRPFEHGRVTPKPGNFTVKIVSHAAVTKDELKGIIKQ
                     PHPNTVVVDARSLEEYLGKEVSGIPRAGHIPSAIHVAWNGFLNKNATVKDLAVIQAGL
                     DEKGLQKNQEVICYCTGGVRSSWLYFVLKLAGYQNVRNYPGSWWEWSRDFACPVEKDF
                     QSLQKVLGVEAPARPS"
     gene            complement(2773..4062)
                     /locus_tag="VLD60_01400"
     CDS             complement(2773..4062)
                     /locus_tag="VLD60_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSB43634.1"
                     /translation="MTGGVKIGLGAVIAVVAVYVYYTEIKPVVIFGLRSDYAHAIPFQ
                     KIPEGLTSLKAESCGQCHREIYEEWKTSIHAHAYEDPFFQAYWKKDKHIWVCLNCHTP
                     LENQQPTLVKEIPRGRVEKAVEEPNPRYDPEYQKESVTCAVCHVRDGVIYGPFEDSAA
                     PHPTKFDPNFRTVQPCYRCHNVVSGPAQFYNVGPCGTYAEYEGKFFMQERGFICQSCH
                     MPEIDRPVATNSPIRRGRQHLWRGGHDPDMIKRAVAVQVNADTASPKPGDRVGFTLTL
                     INAGAGHKVPTGDPDRHFTVEFIVEDQNGQILDRQTSTMGRWIMWQPAIVELYDNRLL
                     PLASREYQFAYRIPDKAEGLKVKTRVQYHILTDGQHEMLQTKYGLTGTDPYRFLIYER
                     EFPLSENLKAALLDEGHQPSVIGRQHEEASCKLKAAS"
     gene            complement(4059..5003)
                     /locus_tag="VLD60_01405"
     CDS             complement(4059..5003)
                     /locus_tag="VLD60_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSB43635.1"
                     /translation="MGKTGLVYHPAYLDHDMGAGHPESPNRLRAIMQHLEQSGMAARL
                     TKIAPRKAEDEWITQVHTPAYVAALHRHAPTSGRVSLDPDTSMSPGSLTAAYLAAGGA
                     LAAIDAIMNKQVDHVFCAVRPPGHHAEAGRAMGFCLFNNVAIAARYIQKKYGLKRVLI
                     VDWDVHHGNGTQHSFEEDASILYFSTHQYPHYPGTGRASERGKGAGEGYTINVPMESG
                     EGDDEYHAVFLKSLVPAADDFKPEFVIISAGFDAHKDDPLASMGLTEAGYADLTHIVA
                     GIAKRHANGRILSLLEGGYNLTALAASVEAHISGLLKA"
     gene            5102..5422
                     /locus_tag="VLD60_01410"
     CDS             5102..5422
                     /locus_tag="VLD60_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB43636.1"
                     /translation="MPNPRIEPLKKVLAMDPSDDVAWFGLGKAYMDDGNFEEAANALR
                     QCITVKPTYSAAYYALAQSLHKLGRIDECREVSATGIAVSTKNGDAMVTKNLEMLKGT
                     LPAE"
     gene            complement(5446..6294)
                     /locus_tag="VLD60_01415"
     CDS             complement(5446..6294)
                     /locus_tag="VLD60_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43637.1"
                     /translation="MARFRPRKTFKLVAHLREGHRVTVPAWKREEEGLSRDLEERWTS
                     LHRHCADQSSHFRLSRLVRRLTHYDYGVRESALAELELATQLIRVGARVTFLPESQAR
                     TADLECHLGHERFFVEVTAMVGSAERQRLPLRGLMVADDELGDEDDRGVILIHRILAR
                     IQQKAKQLADYCDPVILSISIPRADLQGERIGRHETIRLDLKALAGSVTLLLTKFHHL
                     SAVMISLWDVEPLPSKAATRLANVELVERPKQQRAYPRVRVLVQNPGTSAPLGKIPEA
                     VLCDVL"
     gene            complement(6298..6627)
                     /locus_tag="VLD60_01420"
     CDS             complement(6298..6627)
                     /locus_tag="VLD60_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43638.1"
                     /translation="MTNQEINRAVQYVTASTSYGRDTVADILQTGLRELADLAVQSTR
                     QFEREALLEYVSQWTIKRTGQPEPLVREVLGCAGRWLDEVYEEIVRQQSEALESSSKD
                     DDESEPG"
     gene            6781..8580
                     /locus_tag="VLD60_01425"
     CDS             6781..8580
                     /locus_tag="VLD60_01425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HSB43639.1"
                     /translation="MAGSNVTRRKTSKSSTADRWDLTHLIKNPVQQLEQHLSELNAQV
                     AQIESARPSLTSTMASHEFQSILKMSESIAEGSSRLGAFSYLWFSENTKDAKARSFKS
                     HVEERLTALNNRLLFFELWWQGVDEANAARLMADAGDLRYHLETIRRYKPHTLSEPEE
                     KIIHVKNVTGRSAVNTIYDVVTNGFTFTLKTGGKKKVLNREGLMAYVRSPKNSVRQAA
                     YQELYRVFSAQRDLLGEIYKTLVNDWKSENLGLRHFSSPIATRNLGNDVPDQAVDVLL
                     SVCAKNAGIFQTYFMLKSKICKIRPMNRYHLYAPHRTESKKYNYADAVRMVLDAYRGF
                     SPRLADLAQQVIAEKHIDAPTRPGKIGGAYCYSVVPGLSPYVLLNYTGDARDIATMAH
                     ELGHAVHGLMAAHHSVFTFHSTLPLAETASVFGERILSDALMRQESNRKVRQGLLLGQ
                     LDDIYATVLRQAYFVRFEKTAHQMIGEGATGDQLAKSYLAELRQQFGRAVKVPDEFQW
                     EWLTIPHIFASPFYCYAYSFGNLLVLALYRKYQTEGAAFVPKYLDLLATGGSEPPATI
                     LREVSVDMTSEAFWQSGFDTIREMVSQLESTLS"
     gene            complement(9063..9719)
                     /locus_tag="VLD60_01430"
     CDS             complement(9063..9719)
                     /locus_tag="VLD60_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSB43640.1"
                     /translation="MDSCESVIPAPTPIQRRCLSGFLHAILVLFVLTSSFGAAFAEDP
                     ASITIKVLVAYHSLSGNTERMAEAVVEGAKSLPGTAVVMKRVGQVTADDLFSSDAIIV
                     GSPVYWSNMSGEVKTFFDNWQFKFGVYPEFKMKNKVGAAFATGGQISSGKEVTMLTIL
                     AAMLGNQMIIVSGGGAFGASATTEGESLGIDNKELADAQVLGRRVAEVAALIKRVPSP
                     "
     gene            complement(9746..9931)
                     /locus_tag="VLD60_01435"
     CDS             complement(9746..9931)
                     /locus_tag="VLD60_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43641.1"
                     /translation="MKEVRKVHYVKGVFVCPKCRQSYVQEKWIEEWRVRCHRCDYRGS
                     LTEVSVEERMEEETFRR"
     gene            10072..10524
                     /locus_tag="VLD60_01440"
     CDS             10072..10524
                     /locus_tag="VLD60_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSB43642.1"
                     /translation="MNSQWLIAGFLSLSIFLASGVQAEDGLIQVQLPGGVTIHAEVAD
                     TPKKRAEGLMYRERLAKDRGMLFTFGQAQPWTFWMKNTKIPLDIIWMNEKKQIIHIAR
                     NVPICTRTDDSCPQYQPNDPALYVLELGGGEADRLKLEKGAKLQFRVP"
     gene            complement(10533..12719)
                     /locus_tag="VLD60_01445"
     CDS             complement(10533..12719)
                     /locus_tag="VLD60_01445"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HSB43643.1"
                     /translation="MPLQHQTVMDIDQLLDRVKSYNAEADLGILRKAYDFSAKAHKGQ
                     LRRSGEPYLQHPIAVAGVLTSLKTDVTAIVAGLLHDTLEDTVATPEELEKEFGKDVVH
                     LVDGVTKIGKITFRSHEEKQAENFRKMLLSMADDIRVVLIKLADRLHNMRTLEHLSVA
                     KRQSIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYELLRTRVAKRDEDRQQ
                     YIQEVIQLVNGAMQENGLPGQVYGRPKHLFGIYQKMNKQSISFEEVYDLTALRIVTDT
                     KMNCYALVGVIHSLWRPVPGRFKDYIAIPKSNLYQSLHTTVVGPKGEHVEFQIRTEEM
                     HRVAEYGIAAHWKYKEQGQVAERDSKAFGWLHQFVEWHRDLPDNRQFMDSVKLDLFHD
                     VVYVFTPKGIVKELPRGSTPVDFAFAIHTEVGDHCVGAKVNGKIVPLKHQLESGDTIE
                     ILTSQTQTPHKDWLKFVRTSRAKTKIKHWIKIEEQKRSIEIGRRLLESEFRRHGMAPA
                     QVMKSPELSEVAKQSGFDSTDELIAAVGFGHLPTAQVLGKLTASSAGADGAVQEKPVV
                     HKTVAGKSDDKGVKVKGARDVLMQLSRCCNPVPGDRILGYITRGRGLTIHTVDCPNLE
                     ALDYDRERLVEVEWDSSTPSTHAVKVSVMAVDKTGVLANVSSAIAECHANISRAEITT
                     REDRKAVLDFIVEISGTQHLDQVLKAIERVDGVITARRVRAWQEKS"
     gene            complement(12724..14421)
                     /gene="recJ"
                     /locus_tag="VLD60_01450"
     CDS             complement(12724..14421)
                     /gene="recJ"
                     /locus_tag="VLD60_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HSB43644.1"
                     /translation="MKSKLWVFRDIDPSHRAALAQALAISSATASLLMARGVTTPDEA
                     ASWMSSQQPHDPYLIPDMEQAVERLHRAVTSHEPICFYGDYDVDGMTATSVYLSFFDS
                     LGANVRAYVPHRQREGYGLNLGAVQRLHDEGVSLLVTSDCGTTSHREVALAEQLGMDV
                     IVTDHHQTDDQIPPAVAVMNPHRLGARYPFRGLCSAALAYKVAQAYQMRYGEAGLPLE
                     SLLDLVALATIADVVPLHDENRGFVREGLTQLSRGARCGIRALKQVAGVTRDCTAETI
                     AFKLAPRLNAAGRLDHAMLGVQLLTTESPAEAQRLADRLEQLNRERQKIEAEMMSEAV
                     ATVKEQELPAALVLASRRWHVGVVGIAAARLVERFHRPTIVIAIDDKGMGKGSARTVG
                     GFDLYQGLALCRDLVEAFGGHPSAAGVTVRESRLDEFRGRFAAVVAEWATGVATVPTL
                     HVDAEVQLSEVTTKLIQEIGVLNPFGAGNPEPTFAVKGLEVMDSRTVGDKHLKMTVRQ
                     GRSMPFDSIGFGMKSLIERGIPARTPVDLAFIPELNHWNGYHRIQLRIRDVRISAEE"
     gene            complement(14609..14806)
                     /locus_tag="VLD60_01455"
     CDS             complement(14609..14806)
                     /locus_tag="VLD60_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HSB43645.1"
                     /translation="MEKQERKQEPRREPQGKEEVKANPKVVEAGKKLKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            complement(14916..16967)
                     /locus_tag="VLD60_01460"
     CDS             complement(14916..16967)
                     /locus_tag="VLD60_01460"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSB43646.1"
                     /translation="MSDSAIVTFALIAAVAGIAYGLYLAMWVFKLSAGNAKMQEIAKA
                     IQEGAGAYMNRQYKTVGVVAAVLFVVLWGAGAVSDKFGMLTAIGFLVGAGASSIAGYV
                     GMIIAVRANVRTAQAAHNGMNAALTVAFRGGAVTGLLLIGLGLLAITSFYMIAEQIAG
                     QEKAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTLFKGMSAPILYPLALGGVTIFAT
                     IIGILFVKVSPGGEIMPALYKGLFVAGGIAAVAFFPITSKIMEGVGGVSGTSYYLAAL
                     MGLAVTLALVFITDYYTSKSYAPVKEIAKASETGHATNIIAGLAVGMQSTAAPVVVIS
                     AAILGSFWICGGAASGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMSHLG
                     KEVRDITDPLDAVGNTTKAVTKGYAIGSAGLAAVVLFAEYAREVAKGSAASSFDLSNP
                     SVLVGLFLGGMLPFIFGSLCMKAVGQAAGLVVEEVRRQFRTIKGIMEGTGKPEYGTCV
                     DIVTQAAIKKMMIPGLIPVVSPILVGVILGPQALGGVLVGSIVTGLFVAISMTSGGGA
                     WDNAKKYIEEQGKKGTDTHKAAVTGDTVGDPYKDTAGPAINPMIKVINIVALLIVSLL
                     V"
     gene            complement(17181..18116)
                     /locus_tag="VLD60_01465"
     CDS             complement(17181..18116)
                     /locus_tag="VLD60_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43647.1"
                     /translation="MNGLVPFAMHDVRTATARWAWSSTLTGCVLIGGLYGAEPDAYAL
                     EKYGRPLPEFETENVEQMEQGVDESLIAGYLLSAAFISNPSFTARPDNTGRVGLRHML
                     HAETDLYKKYLTFYSDQNFFSDRDNGWIVLSEWDQTYGFTGAVGHWSWRTQYERDAPL
                     DRSGLKQIYADALVNYRIRTNEDWSWWQKMFPKNNLTVYGGLGWLFHNENYFARPDNT
                     GRALFRYVGHFDLNLYREKVLLFGDVNMFTDRSSSNAARPSELDWILGLAMRWRDYEL
                     SFYREQDRPLDRSGFVQEYWAVQVRFAFDVQKQGR"
     gene            complement(18155..18547)
                     /locus_tag="VLD60_01470"
     CDS             complement(18155..18547)
                     /locus_tag="VLD60_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43648.1"
                     /translation="MTWLWRAFPVCLWVASITGCAAVSPNGSGVHPVQQILIGKTKQE
                     LLACAGPPVSESIKEDRVVFLYYKEASLLEESFPGSKSSFAKEHHGCLATLSLQHDRV
                     SEVRYESVPSSYQDEDHCEEIFDPCTGH"
     gene            18672..18926
                     /locus_tag="VLD60_01475"
     CDS             18672..18926
                     /locus_tag="VLD60_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43649.1"
                     /translation="MGLEPNYIVVDGQMFSKAKLALDNHVYKNCQIDDCDLYYSGGQY
                     ELLDTHITNSRLILNHPAKGMYAALQIFKMKSPSSRIISE"
     gene            complement(19062..20315)
                     /locus_tag="VLD60_01480"
     CDS             complement(19062..20315)
                     /locus_tag="VLD60_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSB43650.1"
                     /translation="MRMTEGKSIGVKVALGTLLLLAAMLLSSPSGDRTFAQDAKSQAT
                     IEKAFPNSNKCKRCHERVFEEWETSPLSKSIHSPAFRATLDAFLASPAGKDKALCFRC
                     HAPHVREFPNQVQTFIDQVKSGEPQMDGVACAQCHLIKQVDRTKQPPEPKYESAGSKI
                     LYGPYKDFAQNLAHQSMELNLFKKSDLCLNCHQSVPSAVNLGKSNDLLGNWDQSKAVK
                     SGKDCQSCHMPEQTGESANGEKKRTVANHTFPGRIGKLRQEAAKLDVETKIEGDKTTV
                     IVKVQSLVPHNLPTTHPAWASVVLDLDIKGKNLRTVFAEKRVYGRTYQDAKGQKTNFD
                     FEAVKVLEDTVLKPEELRVETFTFPTPKDTKTFDVEATLSYAPVTGPAPFLQRIEAES
                     SKGSQDPVFQPIEIVKRTENIPVTK"
     gene            complement(20332..21627)
                     /locus_tag="VLD60_01485"
     CDS             complement(20332..21627)
                     /locus_tag="VLD60_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSB43651.1"
                     /translation="MRQTHRLLGGLFVLLMIAGNAFFHERAIAQKGETQAPADWVSEI
                     EKVFIRSEDCKQCHDRHYEEWKGVREQTPDLKTFGRVDAALLHGTSLESPVFRTVLGV
                     WMQTNPTVQERQRCLSCHVPSTTVFPQHAEKIAAQVLDGSPQVEGIGCASCHLIKSVE
                     TTPAPPPTFKIEPGKTLYGPYADPEENLVHPAVQSELFRGANYCASCHFDKVKDVTQK
                     DLPGEILQGTICQDCHMEPSTGSSTSKRGAMTRSIGRHWFRGVVIPGTLLKNRNLQAE
                     WMPRVDIDTTKSASGVEGTALVKVGSLPHSFPDGDPVLKQFFLTITVKDAKGKVLGQE
                     TKQFGLPYDKILRGAIPDPFIKGGHTRKVPFSVALPAGSTATLVEAVLNYSLIPTPEP
                     ALKDKYLATLTNDKDRDSAKKILDEYTQPRILTYRAKNL"
     gene            complement(21634..22026)
                     /locus_tag="VLD60_01490"
     CDS             complement(21634..22026)
                     /locus_tag="VLD60_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSB43652.1"
                     /translation="MLAGLLTGPVVAADQSPMTVEEAAKLGEEFGVVVGEVDEEIQKE
                     LKLQRPQGVAVFEVIGNSRADYAGIKVRSVIKEIDKTEIRNMLDFGRAIKKATRECNF
                     TVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            complement(22193..23023)
                     /locus_tag="VLD60_01495"
     CDS             complement(22193..23023)
                     /locus_tag="VLD60_01495"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSB43653.1"
                     /translation="MARKTTKTSSRPFLLFFLVILIVAGVLGGLGIPLVSSEGMVIRA
                     HVIEGDLPSAPEDAAWGKVPPMTLPLSGQVITRPVWPEPTARALTVRALHNGTEIAFL
                     LEWQDNTKNDRLTPGTFRDGVAIGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDR
                     RAARSTDKQREGVRTFEVIPRRISSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGVWH
                     VVMRRPLSGEEQENEAKLIPGRVQTVSFAVWNGENKERNGQKSVAPWFQLVIDPVAKI
                     "
     gene            complement(23115..24368)
                     /locus_tag="VLD60_01500"
     CDS             complement(23115..24368)
                     /locus_tag="VLD60_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43654.1"
                     /translation="METELQLALGYIETFAGNGKARSTGDGKRAVKAGIPLPHHAALD
                     KEEHWLYFAESGSDRIRRVNLQEGTLHNFAGIGETCYSGDEGPCGEAGLYLPLDVAFD
                     SHNHLYICDSGSNRIRRVDRDTGVITTVVGTGQHGFNGDGPALEVNLTWPAAIAVDKD
                     DVMYIADTQAHRIRRYDPRTGMVSTIAGTWTAEDDAREQPLVARNLVVLSGDAIGIDF
                     SDDHGWLMPVCSDGLDMSMYLDDGRPALEARLYDVVGIAVDSKGNIVFVDKGSNRVRK
                     IDQESGIISTLAGVCRYGYDGDEKPAVRAMLHAPEAVVFDEQDNLYVSDTMNHRVRKV
                     DAITGIISTVAGNGDSGYEDKNMGGCGAARFVAKESAGTLKHGDGLLGVEAVVNSPVG
                     LALDSHGHLYICERGENKIRRVRLW"
     gene            complement(24392..24664)
                     /gene="tatA"
                     /locus_tag="VLD60_01505"
     CDS             complement(24392..24664)
                     /gene="tatA"
                     /locus_tag="VLD60_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSB43655.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAEAAQQQTATPQQAIPATPTQAAPVVEQPAAQPATRA"
     gene            complement(24690..25499)
                     /locus_tag="VLD60_01510"
     CDS             complement(24690..25499)
                     /locus_tag="VLD60_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB43656.1"
                     /translation="MNEPTASAPEQGRAAVDPGDLPLNPDEEIAEIEKLLATEPDDFQ
                     ARCRLGELYFSKGRLDDALVEVKKSIEMAEGLRAEMNRSLAMYYSNLGTIYATKNMTD
                     EAEAEFKHALEVFQYDVLALFNLGRVYADKKNYMEAKGYYERLVEITPEDPIAWYNLA
                     GVYVELDNPQVSDYNTIDMAIQCYLRTLELDPKHLEASFKLMELALNHKKTDLAMKVM
                     EDAVEQSPDEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKRSKESSGS"
     gene            complement(25528..26649)
                     /locus_tag="VLD60_01515"
     CDS             complement(25528..26649)
                     /locus_tag="VLD60_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43657.1"
                     /translation="MLAPGTTIERLTGKLSDQPLLFQYTIKLVLLVAFLELVLYRLVS
                     RLGMHLSKLAADHQWIIPTFTALTEIGQWLLNVVAILLFLGLSVGMANRLASRGLTGL
                     NRFSIPCVGLLLLLTIGFLLVPPTMLGSGIYNTVALVAIVLLMAEYLITHQDWSHRTL
                     GLTYLLGISGWLYYQIVSTFYSFAGTVAAPPMVYESHRGGEALMVLASILVFWAYGRG
                     VSFRSRNQRQRRRALWFWGTAAAIFTAMFFLDYLLNQYDPALANNVRKGADGIGWIFQ
                     FGMGYTFYLPFALYMAGLICWSYTVIKLLTMGRLAGYGLGLMFIAGYALLYSNLTLMV
                     VLGVMLLTMDRYRRDAPDQVPASSATVMGAPDSLVGGHV"
     gene            complement(26731..28512)
                     /locus_tag="VLD60_01520"
     CDS             complement(26731..28512)
                     /locus_tag="VLD60_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB43658.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLAQAGVTNVSQRDLGIEL
                     LDEIVTKSYGVGLYQELVDKQKFLERERTGETGPGSAEHLARVVESLDRFPYLIDRIE
                     LAKETLRGEGFYDMEAYRESLFLIDKWLEVISSVYFPTRLTVVDNQFSTYSIYSSKHL
                     MQVIRDEQQNPYIRLFRERFLPSIIGDRPELIGVSITATSQIIPGLTLCRLIKEAAPD
                     IHLTIGGSIFTRLVDNLRRCPSLFDLTDDIVVFEGETALLELVNQMAGKKDFGKVPNL
                     IYRQNGKINVNQPFYSENVNQLPAPNYDGFPLGRYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQKEPGRTIEELEWLKQRYGVRNFFFTDECFALSPTKRLCQQMIDRHLD
                     IRWTCEMRFEKHLTRELLTSLRDAGCLKIVFGLESFNQRVMDFMKKGIQQEWVRRISN
                     DCVDLGIAVHCYIIVGFPTEKEEEALETMNFIVENKKLNESYGFSCQPCLFDLEKEAP
                     IMSDPGGYGIRRIMRPSAEDLSLGFFYEVQEGMTPDQAERLYQHVYEKVSEVVCELPF
                     NYSMADGLLYIARAKAEERGAVPAAQL"
     gene            complement(28772..>29237)
                     /locus_tag="VLD60_01525"
     CDS             complement(28772..>29237)
                     /locus_tag="VLD60_01525"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSB43659.1"
                     /translation="SAGIWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNPGI
                     WSGNIMSDPTLRVSSVEDLNANGFSTLTTQAHQDVIGNGVWEPSGSVKGGGYSGPTWR
                     VVVKRTLETGDANDTQFKGGMSVPIAFAVWDGSNIERNGMKALSTWFTLKLP"
BASE COUNT         6304 a         8782 c         7795 g         6356 t
ORIGIN      
        1 tggtgagaat cttatcgacg agttccatgt cgccgccgag gcagactcca tcgaacacat
       61 ggtgagaatt ctctggcaac tgattcaaac ggtacagcaa catcacctga ttcggttgga
      121 caggccctcc ggaaaacagc acatggcgtt ggccttccag gatcggtacc tgcggcagcg
      181 cttccgaaat cgacatcgcg gtcggccgat tcacgaccac tcccagcgca ccttcggccc
      241 catgttcaca gaggaggacg accgcttgtc ggaaattcgg atcgcgtaga ttgggcgctg
      301 caatgagaaa aatgcctttc ccgagtggaa ctttcataat ggtaatccta tcgcgccagc
      361 ctgccgcacg caagccgccc cacatagttt gaattcagag cgattccaga gggtgctcaa
      421 acagttcgct caggaaggcc gctccttcgg ggatagactg agtgcctatg cgcccgcgcc
      481 gtcatatcct taacctaaac ctcagcccgc tcaggaaggc gagcaggccg tttcagtacc
      541 ctgctatctg tagagggatt cgcggcgatc gcggagcaaa tcgttgtagc gggtcacgga
      601 tttattccgt gcttcagcgg gatcgatctc cacgatatag agatcttcct ggtcgcgagg
      661 cccacgatgc aaaattgtcc cgcgaggcgt cacgatctcg ctgttgccga taaacgtcag
      721 cggatccttg ccgccccgcg cctcgctccc cgtacggttg caggtaatgg caaagacgcg
      781 gttctccaag cagcgcaccg gcatggaatc aggacagttc ggcaacacga gattggacgg
      841 atgacagatg atctcggccc ccttgagcgc cagagtccgc gccgcctcag gataatacca
      901 gtcgaaacag atcatcacgc cgatcttggc ctgagcgata tcccagacct gaaagcctgt
      961 atcccccggc gtaaaaaaca acgtctcttc aaagaacaga tgggttttcc gataactgcc
     1021 gatcacacct tgcggaccca ccgcgacagc ggagttatag agagacgccc ccgctcgttc
     1081 tgggagcccg gcgacgatga tcaggtgctt acgcttggcg atgtcgagca gtcttcttgt
     1141 ggtcggtcca tcgggaattg gttccgccaa cgcccgcact tcgtcccggg agacaaactg
     1201 atagcctgaa gcacacagct cgggcagaac gataagatcg gccgccgcct cgtccagcct
     1261 ggcggagatt cggtccagat tttcagtcac cttcccgaac tcgggattgt tttggtaata
     1321 cccgacgcgc acggactcac tcccctcaag acaggaacgg gcagggtgct tgtcccctgc
     1381 ccgttctcac atgtgtagcc gtcagatgac agctatcagt tgtttgcccg tacgtgctac
     1441 ttcacttcgg acagatgagt caccgcacct tcagcatgct tggtggcaac atcagcatgg
     1501 cctgccttac cgtgctcgac ggctgcctta agttccttga cgccctcgtc gagatgcggg
     1561 ttttttacgt ccttctgtgc cgcctcggca tgatgtaatg ctccttctgc atgcttcacc
     1621 aaggcatcgg catggccctg cttcccatgg gcagctgctt ctttcgcatg ctcgatggcc
     1681 tcgctgacgt gtttgttgtc ggcctgagcg accagcggca tgccgagcaa ggcgaccgtt
     1741 gccagcatca ggactccacg ttggattctc atcgtcatag actcctcctc tgtgattaag
     1801 ttgcgtgcag tacgagaaaa cgtacgaaag aatagatgtg cgttcacctt acacagccgt
     1861 tttgggactg tcaagaaggc cgggccggag cctccacgcc caacactttc tggagactct
     1921 ggaaatcttt ctccaccggg caggcaaaat cccgactcca ttcccaccac gatccgggat
     1981 aattgcgcac gttctgataa ccggcaagct tcagaacgaa atagagccac gacgaccgga
     2041 caccgcccgt acagtagcaa atgacttcct gattcttctg cagtcctttt tcgtcgaggc
     2101 cggcttgaat cacggcgaga tctttcaccg tcgcgttttt attcaaaaaa ccgttccatg
     2161 ccacatggat agccgaggga atgtgtcctg ctctgggaat accggacact tccttcccca
     2221 aatattcttc caaacttctt gcatcaacaa ccacggtatt tggatgcggc tgtttgatga
     2281 tccccttcaa ctcgtctttt gtcacggctg cgtggcttac gatctttacc gtaaagtttc
     2341 ccggcttcgg cgtcacacgc ccatgctcga acggccgtcg ctcttccacc catttcaccc
     2401 agcctccgtc gagaatgcgc aggttggtat ggccgaggta ctccaacatc cagtacatcc
     2461 ggccttcatc accccaattg tcgaaggggt tggagtagat gacgacctgg ctgtcggcgt
     2521 tgatccccag ggcgcgcatc ttctgttcca tacgtttcag atccggatcg atcaatcctt
     2581 tcgccacggc attgggatca ctatagtcat gccaggtcgt atggaccgcc cccggaatgt
     2641 gcccgccgaa ttcatacgcc gccttgcctc gcacatcgat aatgacgaga ccgggctggc
     2701 ctaactggct ctgcaatgat tcggtgtcaa tgagcaatgg gtgtgtcatg ctgttccttt
     2761 aggagtgcgc tgtcagctcg cggccttcag cttgcaactc gcttcttcat gctgccggcc
     2821 gatgaccgac ggttgatgcc cttcgtccaa taacgcggct ttcaggttct ccgataacgg
     2881 aaattctctc tcatagatca ggaaccgata gggatcggtt cccgtcagcc catacttcgt
     2941 ctgcagcatc tcatgctgcc catcggtcag aatgtggtac tgcacacggg tcttcacctt
     3001 caagccctcg gccttgtcgg gaatccggta ggcaaactgg tattcccggc tggcgagtgg
     3061 taacaggcgg ttatcataca actctacaat ggctggctgc cacatgatcc agcgacccat
     3121 ggtggaggtt tgccgatcca gaatctgccc gttctgatcc tccacaataa actcgacggt
     3181 aaagtggcgg tccggatcgc cggtggggac cttatgacca gcccctgcgt taatgagcgt
     3241 gagcgtaaac cccacccggt cgcccggttt cggggaggcg gtatcggcgt tcacctgcac
     3301 ggcgacagcc cgcttgatca tgtcgggatc gtgcccacct cgccagagat gctgccggcc
     3361 tcggcgaatc gggctattcg tggccacagg ccggtcgatt tcaggcatat ggcagctctg
     3421 gcaaatgaag ccgcgctcct gcatgaagaa cttcccttcg tactcggcat aggttccgca
     3481 gggacccaca ttgtagaact gggccggtcc tgacacgacg ttgtggcagc ggtaacaagg
     3541 ctggactgtg cgaaagttcg ggtcgaactt ggtggggtgc ggcgcagcgg agtcttcaaa
     3601 tgggccatag atgacaccat cgcggacatg gcagaccgca caagtaaccg actccttctg
     3661 atattcggga tcgtaacgcg ggttcggctc ctccacggct ttctcgactc gccctcgtgg
     3721 gatctctttc accaatgtcg gctgctgatt ttcaaggggc gtatgacaat tcaaacagac
     3781 ccagatgtgt ttatcctttt tccaataggc ctgaaagaac ggatcctcgt aggcatgagc
     3841 atggatgctg gtcttccatt cttcgtaaat ttcgcggtgg cactgcccgc aggactcggc
     3901 cttgagactg gtaagtccct caggaatctt ctggaagggg atggcgtggg catagtccga
     3961 ccgtaggccg aaaatgacga ccggcttgat ctcggtgtag tagacgtaga ccgccaccac
     4021 cgcgattacc gcaccaaggc caatcttcac tccgccggtc atgccttcag caatccagaa
     4081 atatgggcct ctactgacgc agccagggcc gtgagattgt acccgccttc cagcaacgac
     4141 agaatgcggc cgttggcgtg gcgcttggcg atccctgcca caatatgggt taaatcagca
     4201 tacccggcct ctgtgagtcc catgctggcc aatggatcgt ctttgtgagc atcgaatccc
     4261 gccgagatga tcacgaactc aggcttgaag tcatccgctg ccggtaccag tgatttaaga
     4321 aagaccgcgt ggtattcatc atcgccctca ccggactcca tcggcacatt gatcgtgtaa
     4381 ccctctcccg cgcccttgcc tcgctcagac gcccggcccg tcccgggata atggggatac
     4441 tgatgcgtgc tgaaatataa gatcgacgca tcctcctcga aactatgctg tgtcccgttg
     4501 ccatgatgaa catcccagtc gacgatcaag acccgtttga gaccatactt tttctggata
     4561 tagcgcgcag cgatcgccac gttgttgaag aggcagaagc ccatggcacg gcctgcttcc
     4621 gcatgatgcc cgggcggacg caccgcacaa aatacatggt ccacttgctt gttcatgatc
     4681 gcatcgatgg cagccagcgc cccacctgcc gccaggtacg cagcggtcag cgagccgggt
     4741 gacatggacg tatccggatc cagcgacact cgtccgctcg tcggcgcgtg tcgatgcagt
     4801 gccgcaacat atgccggagt gtgcacctgt gtaatccact cgtcctccgc tttccgcgga
     4861 gcgatctttg tcaggcgagc cgccattccg ctctgctcca gatgctgcat gatggcgcgc
     4921 agtcgattag gagattcggg atgacccgcc cccatatcat gatcgagata ggctggatga
     4981 tacacgaggc ccgtctttcc catgcgatgc tccaacgcaa gagcgaaaat cgtatcatgc
     5041 ggctatcgcc atagacaatg agcgctcgcc actgctatcg tctatcgcat tggaggaaac
     5101 aatgccaaat ccacgaatcg aaccgttgaa gaaagtgttg gcaatggatc caagcgacga
     5161 cgtcgcctgg tttgggttgg ggaaagccta catggatgac gggaacttcg aggaagcagc
     5221 caacgccctg cggcaatgca tcacggtcaa acctacctat tcagccgcct actacgccct
     5281 tgcacagtca ttgcacaagc taggtcgaat cgatgagtgt cgtgaggtct ccgccaccgg
     5341 cattgctgtc tccaccaaaa acggcgatgc aatggtgacg aagaatctgg agatgctgaa
     5401 agggaccctt ccggccgagt aatttccgca cgaccagttc acaccttaca gtacatcaca
     5461 caacaccgct tccgggattt tgccgagcgg agcgctcgtt ccgggattct gaaccaagac
     5521 tcgcacacgc gggtaggctc gctgttgctt cggacgctcc accagttcga cgttggctaa
     5581 cctcgttgca gctttcgacg gcagcggttc gacgtcccag agggaaatca tcacggcact
     5641 caggtggtga aacttcgtca gcaacagggt gactgatccg gccagcgctt taagatccaa
     5701 cctgatcgtc tcatgccgac ctatcctctc accctgcaag tcggcccgag ggatggagat
     5761 gctgaggatc acgggatcgc aatagtctgc cagctgcttc gccttctgct gaatgcgggc
     5821 aagtatgcga tggattaata tgacaccccg atcgtcctcg tctcccaact cgtcatcagc
     5881 aaccatcaat cctcgaagag gcagtcgctg ccgttcggcc gatcccacca tcgccgtcac
     5941 ttcgacaaaa aatcgctcgt ggcccaggtg acattctaaa tcggcggtcc tcgcctgaga
     6001 ctccggcagg aacgtcacac gtgctcctac gcggatcaat tgcgtggcaa gctccagttc
     6061 ggcaagggct gactctcgaa ccccatagtc gtagtgagtc aaccgacgga ccaggcggct
     6121 gagcctgaaa tggctcgact ggtcggcgca atgtcgatgg agactggtcc aacgttcctc
     6181 gagatcgcga gatagccctt cctcctcgcg cttccaggcg ggaactgtaa cacgatgccc
     6241 ttcacgcaga tgcgcgacca gcttgaacgt cttccgtggg cgaaaccttg ccatcagtca
     6301 ccctggttcc gactcgtcgt catccttcga tgaggattcg agcgcctctg actgctgcct
     6361 gacaatttct tcgtagactt catccagcca acgccccgcg caacccaaca cttctcggac
     6421 caacggctcc ggctgcccgg tccgcttgat cgtccattgc gagacatatt ccaataatgc
     6481 ctctcgctca aactgccgcg tcgactgaac ggccaggtcg gccagttcgc ggagccccgt
     6541 ttggaggata tcggcgacgg tgtcccgtcc gtatgacgtg ctggccgtga catattgcac
     6601 ggctcggttg atttcctgat tggtcatctg acgctccgct ggaatggatt ttcacatccc
     6661 gtcgaatgcc tgtcaacgcg ggcttactcc ccttcggcct cagatatttt cctcttctca
     6721 gagcttggcg tgatggtaaa atctggcctc cttgaccgca gagacatgaa ggagacgcgc
     6781 atggccggaa gcaacgtgac acgacgcaag acctccaagt cctcaacagc cgatcgatgg
     6841 gacctgacgc acctcatcaa gaatcccgtc caacagctgg agcagcacct ctctgagctc
     6901 aacgcacaag tggctcagat cgaatccgcc cgcccgagcc tgacatcgac aatggcaagc
     6961 cacgagtttc agtcgatcct gaagatgagc gagtcaatcg ctgaaggctc ctctcggctc
     7021 ggcgcctttt cttatctttg gttctcagaa aacaccaaag acgcaaaggc acgttcgttc
     7081 aaatcgcacg tagaggagcg gctgaccgcc ctgaacaatc gccttttgtt ctttgaactg
     7141 tggtggcagg gagtggatga ggccaacgcg gcgaggttga tggccgacgc cggcgatctc
     7201 cgctatcact tggaaaccat caggcgctat aaaccccaca ccctctcgga gccggaagag
     7261 aaaatcattc acgtaaagaa cgtgaccggc cggagtgccg tgaacacgat ttacgatgtc
     7321 gtgacaaacg gttttacatt cacgctcaag acgggcggaa agaagaaagt cctcaatcgc
     7381 gaaggcttaa tggcctatgt ccgcagcccc aagaattccg tgcgacaggc tgcctatcag
     7441 gaactctatc gtgtgttctc cgcccagcgt gatttgctgg gagaaatcta taaaaccctg
     7501 gtcaacgact ggaagtccga gaacctcggc cttcggcatt tctcctctcc cattgccacg
     7561 cgcaatctcg gcaacgatgt gccggaccaa gccgtcgacg tcttactctc tgtatgtgcc
     7621 aagaatgcag gcatcttcca gacctatttc atgctcaaat ctaagatctg caagatcagg
     7681 cccatgaacc ggtatcacct ctatgccccg catcgcacag aatcaaagaa gtacaactat
     7741 gccgatgcgg tcaggatggt gctcgacgcc tatcgtggct tctcgccgcg cctggcggat
     7801 ctggctcaac aggtaatagc cgagaagcac atcgatgcac cgacgagacc cggcaagatc
     7861 ggcggagcct attgttatag cgttgtgccg gggctctccc cttacgtgct gctgaactat
     7921 acgggcgatg cgcgcgacat cgcaacgatg gcccacgagt tgggccatgc cgtacacggc
     7981 ttgatggcgg cacatcattc ggtgtttacc ttccattcca ccttgccgtt ggcggaaacc
     8041 gcttctgtgt tcggcgagcg catcctttcc gatgccctca tgcgtcagga atccaacagg
     8101 aaggtccggc aggggctgct gctcggccaa ttggatgaca tctatgccac ggtcctgcgg
     8161 caggcctact tcgttcggtt tgaaaagacg gcgcatcaga tgatcggcga aggcgctaca
     8221 ggcgatcagc tggccaaatc ctatctcgct gaattgcgcc agcaatttgg cagagctgta
     8281 aaagtgcctg atgagttcca atgggagtgg ctgaccatcc cccacatttt tgccagcccg
     8341 ttctattgct acgcctacag cttcgggaat cttctcgtcc tcgccctcta ccgcaaatat
     8401 caaacagagg gtgcagcatt cgtccccaag tatctcgatc tcctggcaac gggaggatcg
     8461 gaaccgcctg cgaccattct tcgcgaggtt agcgtggaca tgacctctga ggcgttctgg
     8521 caatcaggct tcgatacgat cagagagatg gttagccagc tcgaatcaac cctctcgtaa
     8581 ctccggggaa tcccgaaacg ccgcggccac ggcggatttt ttgagacttg acagggaggt
     8641 caaaggagat cataatgaga atgcttctca taatcgttgt cagcctactc accggcgagg
     8701 cgttgtatgt atgtctgttt gtgcaaagga atcactgaat ctgatgtccg tgaagcagga
     8761 cgggatggga ttgtgatgcc ctgccagctc aaagccaagt ttggcctgaa agacccgggc
     8821 tgttgcggtc gctgctccaa gaacattctg gagttcgcgg aactcgctgc aagcgaacac
     8881 caactcccca cccctcttac tattcgccga tagcccctct tcaccccgac agataactga
     8941 ttcgacccag ctccgtctca acgaagcagc gcgcgaattc cagtcaggat tctacaagac
     9001 agaactggct tgagcggtac tcaatgccgg cgatagaagt cagaaagagt gagctggtga
     9061 ggttatggag agggaacccg tttgatgagg gctgcgactt ccgccacgcg ccgccctaac
     9121 acctgagcat cggccaattc cttattgtcg atcccgagac tctccccttc agtcgtggct
     9181 gatgcgccga aggcgccccc cccgctgacg atgatcatct gattgcccaa catcgcggca
     9241 agaatcgtca acatggtcac ttctttgccg ctcgaaatct gcccacctgt ggcgaaggcc
     9301 gcaccgacct tgttcttcat cttgaattca ggatacacac cgaacttgaa ttgccagttg
     9361 tcgaagaacg tcttcacttc ccccgacata ttcgaccaat agaccggtga gccgacaatg
     9421 attgcatcgg aggaaaacaa gtcatccgcc gtcacttgcc ctacccgctt catcacgacg
     9481 gccgtccctg gcagactttt cgccccttca acaacagcct cggccatacg ttcagtattt
     9541 cctgacaaag aatggtaagc gaccagcact ttgatggtga ttgaagccgg atcttccgca
     9601 aatgcggcgc cgaagctact ggtcaggacg aaaagaacga gaatggcgtg caggaaaccg
     9661 ctaagacaac gtcgctgaat tggggtaggc gcgggaatca cggattcaca ggaatccatt
     9721 tatgatgcat gtgacaggaa atggatcacc gacgaaacgt ttcctcttcc atccgctctt
     9781 cgacggatac ttccgtcaac gatccacggt agtcgcagcg atggcatcga acccgccatt
     9841 cttcgatcca cttctcctgg acatacgatt gccggcactt cggacacaca aacacgccct
     9901 tgacgtaatg tactttccga acttctttca taccgtcctc ctggctattg cggcacagga
     9961 tcgcataggc attgtcggga ttgcaaggcg ggcacaaccc cggtggcctt gcccggtcat
    10021 ggctccaggc gatgtcttga ctcaattccg gacgcccgat aggatgcccg cgtgaatagc
    10081 caatggctca tagccggttt tctgtccttg tcgattttcc tcgcctccgg cgtccaggct
    10141 gaggatggat tgatccaagt ccagcttcct ggcggtgtca caattcatgc agaggtcgcg
    10201 gataccccga aaaagcgagc cgaggggctg atgtatcgcg agcgcctggc gaaagaccgt
    10261 ggaatgttgt ttacgttcgg ccaagcccaa ccctggacct tctggatgaa aaacaccaag
    10321 atcccgctcg acatcatttg gatgaatgag aaaaaacaga ttatccatat cgcacgaaac
    10381 gttcccattt gcacgagaac ggacgacagc tgtccccagt accaacccaa cgatccggcc
    10441 ctctatgtat tggaactcgg cggcggggaa gctgatcggc tgaaactcga gaagggagca
    10501 aagctgcagt ttcgcgtgcc ctaggcacgt tgctaggact tctcctgcca ggcccgtacc
    10561 cggcgggccg taatcactcc atcgacccgt tcgatggctt ttaacacctg atccaagtgt
    10621 tgggtgccgc taatttccac gataaagtcg agaacggcct tacgatcttc ccgcgtcgta
    10681 atttcagcgc gactgatatt ggcatggcat tctgcaatgg cggatgaaac gttcgccaga
    10741 accccggtct tgtccaccgc catgaccgac accttcacgg catgcgtgct cggagtgctg
    10801 gaatcccatt ccacctcaac caaccgttca cgatcataat caagcgcttc gagattcggg
    10861 cagtcaaccg tatgaatcgt caagcctctc ccgcgcgtaa tatagccgag gatccgatca
    10921 ccgggaaccg ggttacaaca gcgtgacaac tgcatcagca catcgcgcgc gcccttgacc
    10981 ttcacgccct tgtcgtcgga tttgccggcg accgttttgt gcacaacagg cttctcttga
    11041 acggcaccgt cggcccccgc tgaagaagcc gtcagctttc caaggacctg agccgtcggc
    11101 agatgaccga atcccaccgc agcgatcaat tcgtccgtgc tatcgaagcc ggattgcttg
    11161 gccacctccg acagttctgg cgacttcatg acctgggccg gcgccatgcc atggcgacgg
    11221 aattcagact ccaacaatcg gcgcccgatt tcgatactcc gcttctgttc ttcaatcttg
    11281 atccaatgct tgattttggt cttcgcccga gacgtcctaa caaacttgag ccagtcttta
    11341 tgcggcgtct gtgtctgcga cgtgagaatt tcgatggtat ctccgctttc gagctgatgc
    11401 ttgagcggca cgatcttacc attcaccttg gctccgacgc agtgatcccc cacctcggtg
    11461 tgaatggcga aagcgaaatc caccggcgtc gagccccgcg gcagttcctt cactattccc
    11521 ttcggcgtga acacatagac gacgtcatga aacagatcta gcttgaccga atccatgaac
    11581 tggcgattgt ccggcaaatc cctgtgccat tcgacgaatt gatgaagcca cccgaaggct
    11641 ttggaatcgc gctccgcgac ctgtccctgt tccttgtatt tccaatgtgc ggcaataccg
    11701 tattccgcca cccggtgcat ttcttccgtg cggatctgaa attcgacgtg ctcaccctta
    11761 ggcccgacca ccgtcgtatg cagcgactgg tagaggttgg acttgggaat ggcaatgtaa
    11821 tctttgaatc gcccgggaac gggacgccac agagaatgga tcacgccgac gagagcgtaa
    11881 caattcattt tcgtgtccgt cacgatccgc agggccgtca ggtcgtagac ttcttcgaac
    11941 gagatcgact gcttgttcat cttctggtag atcccgaaca gatgtttggg acggccatac
    12001 acttgtccag gcaagccgtt ctcctgcatg gcgccgttca caagttgaat gacttcttgt
    12061 atgtattgtt ggcggtcttc atcacgtttc gccacgcgag tgcgcagcag ctcatagaca
    12121 tccggcttga gatgcttgag gcagaggtcc tccagctcgt tcttgatcca tccgatgccg
    12181 agccggttgg ccagcggggc ataaatctcc agcgtctcct gtgcaatgct ctggcgcttg
    12241 gccacgctca aatgctccag ggtccgcata ttatggagcc ggtcggcaag cttgatgagg
    12301 acgacgcgaa tgtcgtccgc catggacaag agcatcttgc ggaaattctc cgcctgtttt
    12361 tcctcatgac tccgaaaggt aatcttgccg atcttggtca caccgtcgac gaggtggacg
    12421 acgtctttcc caaactcttt ttccagttct tcaggggtcg cgaccgtatc ttcgagggta
    12481 tcgtggagca gaccggcaac aatggcggtc acatcggtct ttagcgaagt cagcacgcct
    12541 gccaccgcaa tgggatgttg taaatacggt tctccggaac gtcgcagctg gcccttatgc
    12601 gctttggctg aaaaatcgta ggccttgcgc agaatgccga ggtccgcctc cgcgttatag
    12661 ctcttgacgc gatccagtag ctgatcgata tccatcaccg tttgatgttg aagcggcatc
    12721 aggctactcc tctgcactga tgcggacatc gcggatacgg agctgaatac ggtgatagcc
    12781 gttccagtga ttcagttccg ggataaaggc cagatcgacc ggcgtccgag cgggaatccc
    12841 gcgttcgatt aatgatttca tcccgaaacc gatactatcg aaaggcatcg aacgcccctg
    12901 gcgtaccgtc atcttcaaat gcttgtcacc gaccgtgcgg gagtccatga cttcgaggcc
    12961 cttaacggca aacgtcggct ccggattgcc cgccccgaat ggattgagca cgccgatctc
    13021 ttggataagc ttggtcgtga cctccgacaa ctgcacttcc gcatcgacat gtaaggtcgg
    13081 aacggtggcc acccctgtcg cccattcggc cactactgcg gcaaatcgac cacgaaattc
    13141 atcgaggcgg gattcacgca ctgtgacccc cgccgcgctg ggatgcccgc cgaacgcttc
    13201 aacgagatcc cggcacagcg ccagtccttg atacagatca aatccgccga cggtgcgcgc
    13261 cgatcccttt cccatgcctt tgtcgtcgat tgcgattaca atcgtcggac gatggaagcg
    13321 ttcaaccagc cgtgctgccg caatgccgac aacacccacg tgccagcgac gagaggccag
    13381 cacgagtgcc gccggcagct cctgttcttt gacggtcgcc acggcctctg acatcatctc
    13441 ggcttcaatc ttctgccgtt cgcgattcag ctgttccaat cgatcggcca atcgctgcgc
    13501 ttccgccggc gactctgttg tgagcaattg cacgcccaac atggcatggt ccaaccgtcc
    13561 ggcggcatta agccgaggcg ccagcttgaa cgcgatcgtc tcggccgtgc agtctcgcgt
    13621 gactcctgcc acctgcttca acgccctaat accgcatcgc gcaccacgag acaactgcgt
    13681 aagcccctcc cggacaaacc ctcgattttc gtcgtgcaac ggaacgacgt cggcaatggt
    13741 cgccaacgcg acgagatcca acagcgattc gaggggaagc cccgcttcgc cgtaccgcat
    13801 ctggtaggcc tgcgcgactt tgtaggccag agcggcggag cagagtccgc gaaatggata
    13861 gcgcgcaccg agtcgatggg gattcatcac agccacagct ggcggtatct ggtcgtcagt
    13921 ctgatggtga tccgtcacaa tgacgtccat ccccagctgc tcggccagcg ccacttcgcg
    13981 atgggaggtc gtgccgcaat ccgacgtgac gagtaatgag actccctcgt catgcagccg
    14041 ctgcacggcg ccgagattga gcccgtaccc ttcccgctga cgatgcggca cataggcccg
    14101 cacgttggcg cccaatgagt caaaaaagga cagatacacg ctcgtggctg tcatcccatc
    14161 gacgtcataa tcgccgtaaa aacaaatcgg ctcgtggctc gtgacggctc ggtgcaggcg
    14221 ttcaacggcc tgttccatgt ccggaatgag gtagggatcg tgcggctgct gagaggacat
    14281 ccaggaggct gcttcgtcag gtgtcgtcac accgcgagcc atgagcaatg aggcggtcgc
    14341 agaggaaatc gccaacgcct gcgcgagagc tgcgcgatga ctgggatcga tgtcacgaaa
    14401 gacccagagc ttggacttca ttacgacctc acgaaggagc ctgagcctca aagatgcgta
    14461 accgcctgag atataagggg atactataag gtcatcccgc ctttgtcaaa ccagcagcgc
    14521 aggagctttg caacaattgt cgcagaaaat gagataggag gcggagaggg aaaaaacaac
    14581 gaggggaagt gggctgtcaa cttctcagct actccccccc tttttgcacg tagttcttga
    14641 cgaattcttc ggcgtttttt tcgagcacgt catcgatttc gtcgaccagc ttgtcgatat
    14701 cttccttcag cttcttgccg gcctcgacga ctttcggatt ggccttgacc tcttcctttc
    14761 cctgcggctc tcgtctgggc tcttgcttgc gctcctgttt ttccattcga gcacctcccg
    14821 tgaatctggg aaactctcgt ggatcagcca ccagcgtgat cccgtacggt caccttaccc
    14881 cacgattctt tcaatcgtca agcagaggcg cattatcaga cgagcagcga gacgattaag
    14941 agcgcgacaa tattgatgac tttgatcatc gggttgatgg ccggccctgc cgtgtccttg
    15001 tacggatcgc cgactgtatc gccggtcact gccgccttgt gcgtgtccgt ccccttcttg
    15061 ccctgctctt cgatgtactt cttggcattg tcccaagccc caccgccgct cgtcatggag
    15121 atggcgacga acagccccgt cacaatgctc ccgaccagca cgccgccgag tgcttgcggg
    15181 ccgagaatga ccccgacaag gatgggggac acgactggaa tcaatcccgg gatcatcatt
    15241 tttttgatcg ccgcctgcgt cacgatatcc acgcaagtgc catactccgg cttgcccgtc
    15301 ccttccatga ttcctttgat tgttcggaac tggcgccgca cttcttccac cacgagaccg
    15361 gctgcctgcc cgacggcctt catgcagagc gagccgaaga taaacggcag catcccaccc
    15421 aaaaagagtc caaccaggac agaaggattg gaaagatcaa agctgctggc tgcactgcct
    15481 ttggcgacct ctctcgcata ctcagcgaag agcaccacgg cggcgaggcc ggctgatccg
    15541 atcgcatacc ccttcgtcac cgccttggtt gtattcccga ccgcatcaag cggatcggtg
    15601 atgtctcgca cttctttccc gaggtgagac atctcggcaa ttccgccggc attgtcagta
    15661 atgggcccga aggcatcaat ggccacaaca atgccggcca ttgataacat cgagacggcc
    15721 gccactgcga ctccgtacaa accgcccgag gccgcgccgc cgcagatcca gaaactcccg
    15781 agaatcgcag cggaaatcac gacgaccgga gccgctgtcg actgcatccc caccgccaga
    15841 cctgcaatga tgttcgttgc atggcccgtt tcgctggcct tcgcaatctc tttcaccggc
    15901 gcatagctct ttgaagtgta atagtcggta atgaacacca gcgccagcgt cacggccagc
    15961 cccatcaacg ccgcaaggta atagctcgtg ccgctcacgc cacccacacc ttccatgatc
    16021 ttcgaggtga tcgggaagaa cgccaccgcc gcaatgccgc cggcgacgaa caggcccttg
    16081 tataatgccg gcataatctc gccgcccggg ctgactttca cgaacagaat cccgatgatc
    16141 gtcgcaaaga tcgtcacgcc acccagtgcc agaggataga gaatcggcgc gctcattccc
    16201 ttgaacagcg tgaacgccaa gaccatcgcc gccacggtcg tcacggcgta ggtctcgaac
    16261 aggtctgccg ccatgccggc acaatcgccg acgttatcgc cgacgttatc cgcgatgact
    16321 gctgggttac gtggatcgtc ttccggaatc cccgcctcga ccttcccgac aagatcagcg
    16381 cccacgtccg cagccttcgt atagatgccc ccgccgacac gagcaaacac ggagattaaa
    16441 ctgccgccga aaccgagact caacaacgca tggattgctt tctcctgacc cgctatttgt
    16501 tcagcaatca tgtagaaact ggtgatcgca agaaggccca agccgatcaa cagcaatcct
    16561 gtcacggctc cgccacggaa ggcgaccgtc aacgcggcat tcatgccgtt atgcgccgct
    16621 tgggccgtcc gcacgttggc gcgaaccgcg atgatcatgc cgacgtagcc ggcgatcgac
    16681 gaagcgccag ccccgacgag gaaaccgatt gccgtcagca tcccgaactt atcggagacc
    16741 gcaccggcgc cccaaaggac gacgaacaac acggcagcga cgacaccaac cgttttgtac
    16801 tgacgattca tgtaggcacc ggcgccctcc tgaatggcct tggcaatttc ttgcatcttg
    16861 gcattcccgg cactgagttt gaacacccac atggccaggt acaggccata ggcaatgcct
    16921 gcgacagccg ctattaacgc aaaggtgaca atcgctgagt cgctcacggg ataccctcct
    16981 caaaggttaa acgggcttct tatgtcacac ggttccccat ggcttcaacc catcaaacgc
    17041 acgtgagaca tcgggggaaa ccgcggggtg gtgcagagtc gatgtgcagc taactagtcg
    17101 aaaaactggg ccattctata caggtaggcg catgggatca agatgaaatt cggcacctct
    17161 gtatcctgtc gtccgagcaa ttatcggccc tgtttttgta cgtcgaaggc gaagcggact
    17221 tgaacggccc agtattcttg aacaaagccg gaacggtcga gggggcgatc ctgctcgcgg
    17281 taaaagctga gctcatagtc acgccagcgc atggcgagac cgaggatcca atccagttct
    17341 gatggcctgg cggcgttcga agagctgcga tcggtaaaca tgttgacgtc gccgaagagt
    17401 aagactttct cgcgatacag attgagatcg aaatggccga cgtagcgaaa gagggcacgt
    17461 cccgtattat caggccgcgc gaaatagttc tcattatgga agagccagcc caatccgcca
    17521 tacaccgtta gattgttttt aggaaacatc ttttgccacc aggaccaatc ctcattggtg
    17581 cgaattcgat agttgaccag cgcatcggca tagatctgct tgaggccgga acggtccagc
    17641 ggggcgtctc gttcatactg ggtacgccaa ctccagtgac cgaccgcccc ggtgaagccg
    17701 taagtctggt cccactcgct caatacaatc cacccgttgt cgcggtcgga aaagaagttc
    17761 tggtctgaat aaaaggtcag gtacttctta tagaggtccg tttcggcatg gagcatatgg
    17821 cggagcccga cacgtcccgt gttatcgggt ctggccgtaa acgagggatt cgagataaag
    17881 gccgcactga gaaggtaccc tgcaatcagt gactcatcga caccctgctc catctgctcg
    17941 acattctccg tctcaaactc aggcagcggt cgtccgtatt tttcgagcgc ataggcatca
    18001 ggctctgctc cgtagagccc tccgatcaag acgcagccgg tcaatgtgct gctccaagcc
    18061 catcgagctg tggctgttct tacgtcgtgc atggcgaagg gtaccagccc gttcatttct
    18121 atcttggtca gttcgtgtgg ttccgcggca gccgctagtg gccggtacag ggatcaaata
    18181 tctcttcgca atggtcttcg tcctggtagg aactcggcac actttcgtag cgtacctcgc
    18241 tgacgcgatc gtgctggaga gagagggttg cgaggcaccc atgatgctcc ttggcaaagc
    18301 tgctcttcga accaggaaag gattcttcca ggagagaggc ttctttgtag tagaggaaca
    18361 cgaccctgtc ctcttttatg ctttcgctga ccggcggacc ggcgcaggcc aacagttcct
    18421 gcttcgtttt tccaatcaaa atttgctgga caggatgaac tccagaccca ttgggcgaga
    18481 ctgccgcaca tcccgtgatg ctggccaccc acaggcacac cggaaaggct cgccataacc
    18541 atgtcatgta ttgagtcata gccgggtcgg agaatactcc agaagaatcg gaaaaggaag
    18601 tcgcagaagc ggtcctggct gtttgcggag aggaagggac tttgttatag tccgcgccgg
    18661 aggcggcagc gatgggactg gaaccaaact acatcgtggt cgatgggcag atgttcagca
    18721 aggcgaaact agctttggac aatcacgtgt acaagaactg tcagatcgac gattgcgacc
    18781 tctactacag cggcggtcag tacgaactcc tcgataccca cattaccaat tcgcggctca
    18841 tcctgaacca tccagccaag ggcatgtacg ccgctctgca gatcttcaag atgaaatcac
    18901 ccagttccag aattatctcc gaatagtcct ctctctattt ctcttcgata ttgccaatca
    18961 gtcatcgcga gggccatgcc ttcggaaatt ccgcatggca aggcccggaa gggtcgcatt
    19021 gagaagccag gaatagacct gggccacgcc acgtctccct gctatttagt aacaggaata
    19081 ttttccgtcc gcttgacgat ctcgatcggt tggaagaccg gatcctgcga gccttttgaa
    19141 gattcggctt cgatccgctg caggaaggga gcaggccccg tgacgggggc gtagctgagg
    19201 gtcgcttcaa catcgaatgt cttggtatct ttcggtgtgg ggaaggtaaa tgtttcgact
    19261 cgaagctctt cgggtttcaa caccgtatct tccagcacct ttaccgcttc gaaatcgaaa
    19321 ttcgtctttt ggcccttggc atcctgatag gttcggccat agacacgctt ttcggcaaac
    19381 acggtcctga gatttttccc cttgatatcc agatccaaca cgacgcttgc ccaagccgga
    19441 tgggttgtcg gtaaattgtg aggcaccaga ctttgcacct tcacgattac ggtcgtcttg
    19501 tctccttcga tctttgtctc cacatcgagc ttggcagctt cctggcgcag tttgccgata
    19561 cgacctggga aggtgtggtt ggcgaccgtc cgctttttct caccgttggc cgattcgccg
    19621 gtttgctccg gcatgtgaca ggactgacaa tccttgccgg actttaccgc tttgctctga
    19681 tcccagtttc caagcagatc gttgctcttc cccagattga ccgccgacgg gaccgactga
    19741 tggcagttca ggcagaggtc cgacttctta aagaggttca gttccatcga ctgatgcgcg
    19801 agattttgcg cgaagtcctt gtaggggcca tacaggattt tgctgccggc ggactcatac
    19861 ttcggctccg gcggctgctt cgtccgatcg acctgcttga tcaggtggca ctgggcgcat
    19921 gcgaccccgt ccatttgcgg ctcgcctgac ttcacctgat cgatgaacgt ctgcacctga
    19981 ttgggaaatt cccgcacatg cggcgcatgg cagcgaaaac agagcgcttt gtctttgccc
    20041 gccggcgagg cgaggaaggc gtccaaggtt gcgcgaaacg ccggtgaatg aatcgatttc
    20101 gacagtggtg acgtttccca ttcttcaaag acccgttcat ggcagcgttt gcacttattg
    20161 gaattcggaa acgccttctc gatggtcgct tgggatttgg cgtcctgggc gaacgtgcga
    20221 tcaccagacg gactggagag cagcatcgcc gcgagcagta gcagggtccc aagcgcgacc
    20281 ttcacaccaa ttgacttgcc ttcggtcatt cgcatgatcc gtcttagctg cttagaggtt
    20341 cttggcacga taggtgagta tgcggggctg cgtgtactca tcaagaatct tcttcgcact
    20401 gtcccggtct ttgtcgttgg tcagcgtcgc cagatacttg tccttcaacg ccggctctgg
    20461 cgtggggatc aacgaataat tcaggacggc ctccaccaac gtcgcagtcg aaccggccgg
    20521 cagggcgact gagaacggaa cctttctcgt atgccccccc ttaatgaagg gatcagggat
    20581 ggccccgcga agaatcttgt cgtaaggcaa cccgaactgc ttggtctcct gccccaacac
    20641 cttccccttc gcatccttca cggtgatggt aagaaagaat tgtttcaaca cgggatcgcc
    20701 gtccgggaaa ctgtggggga ggctcccgac cttcacgagt gcagtccctt cgacgccaga
    20761 cgcggacttc gtcgtgtcaa tgtccacgcg cggcatccat tctgcctgga gatttcgatt
    20821 cttcagcaac gtgccaggaa tcaccacgcc acgaaaccaa tggcgaccaa tggatcgcgt
    20881 catcgcacca cgcttggatg tcgaactccc cgtcgagggt tccatgtgac agtcttggca
    20941 gatggtgccc tgaagaattt ctcccggcag atctttctgc gtgacgtctt tgaccttgtc
    21001 gaaatggcac gacgcgcagt agttggcgcc gcggaagagt tcggattgca cggcgggatg
    21061 aaccaagttc tcttccggat cggcataggg cccatagagt gtcttgcccg gctcgatctt
    21121 gaacgtcggc ggcggtgccg gtgtcgtttc gacggacttg atcagatggc acgaggcgca
    21181 cccgatgcct tcgacctgcg ggctgccatc cagcacctgg gcagcaatct tttcagcatg
    21241 ctgcgggaat accgtggttg acgggacatg acaagacaaa caacgctgcc gctcctggac
    21301 ggtcggattg gtttgcatcc agacacccag gactgtccgg aacaccggcg actccagtga
    21361 cgtcccatgc agaagcgcgg catccacgcg tccgaacgtc ttgaggtccg gcgtctgttc
    21421 ccgcacgccc ttccactctt cataatggcg atcgtgacat tgcttgcagt cttcggaccg
    21481 gatgaagacc ttttcgatct ccgataccca gtcggccgga gcttgggtct cgcctttttg
    21541 cgcaatcgcc cgttcgtgaa agaaggcatt gccggcgatc atgagaagga cgaacagccc
    21601 tcccagcagt ctgtgtgtct gacgcatatg cgattagtcc cgcttgcagc cgacaaaatg
    21661 gaaattgacg ccccaaccga ccggatcgcc ggggtccgcc ggctcatagg tcccgacggt
    21721 aaagttgcac tccctcgtcg cctttttgat agcccttccg aaatccagca tgtttctgat
    21781 ttcggtcttg tcgatttcct tgatcaccga acgaactttg attcccgcat agtcggcccg
    21841 tgaattacca atgacctcga agacggcgac gccttgcggg cgctgtaact tgagctcctt
    21901 ttgaatctcc tcatcaactt ctccgaccac gacgccaaat tcttcaccca gcttggcagc
    21961 ctcctcaacc gtcatcggtg actggtccgc cgcgactaca gggccggtca agagcccagc
    22021 cagcatgcat gccagcacag ccacgcacaa gaaaaaggcc cttccaccgc gaagggcctt
    22081 tattggaaaa ccgccggacg tcgtgcgagt accccccact gtctgcgcct cttgttcacg
    22141 caaccgaatt cagcagccag ccccaaggcc tcgcttcatc tcaccaaaga atctagattt
    22201 tcgcaaccgg atcgatcacg agttgaaacc agggcgcaac cgacttttgg ccgttccgct
    22261 ctttgttctc gccgttccag acggcaaacg aaacggtctg cacgcggccg gggatcaact
    22321 tggcttcgtt ttcctgctcc tcacccgata acggcctgcg catgaccaca tgccagaccc
    22381 catctttcca cgtcgccttg ccctgcactc gaccttgctt ttccttcgtg gtcagcgtgc
    22441 tgaatccccc gccgatgagg tcctctaccg aggagatgcg gcgcgggatc acctcaaatg
    22501 tccggacccc ttctcgctgt ttatcggtgc tccgtgcggc ccgacgatcg atatcgcttt
    22561 gccaatcagc tttccaatgc caaatgttga tgtaatgatc gagctgtccc atgcagaaga
    22621 acgcgggcgc atcgccaagg ggaaggccga ttgccactcc atcgcgaaac gttccgggcg
    22681 tgaggcgatc gttcttggta ttgtcctgcc attcgagcag gaacgcgatc tccgtgccgt
    22741 tgtggagtgc ccgcaccgtc aaggcacgtg cggtcggctc aggccagacc ggcctggtaa
    22801 tcacctgccc gcttaacggc agcgtcatcg gaggcacttt tccccaggcg gcatcttcgg
    22861 gggccgatgg cagatcccct tcgatcacat gagcccgaat gaccatgcct tcggagctga
    22921 cgaggggaat gcccaggcct ccgagaacac cagccacgat cagaattacg aggaagaaca
    22981 gcaggaaagg gcgtgaagag gttttcgtcg tcttgcgcgc catgcagggg cccctgttga
    23041 ctaagctcac cgggtccgac gatgtcaaaa tagcacaaaa ttcctgcggg accaatccga
    23101 gcggctgcgt gtgcttacca aagccgcacg cggcgaattt tgttttcgcc ccgctcgcag
    23161 atgtagagat gcccgtggga atccaatgcg agcccgacag gagaattcac gaccgcttcg
    23221 acgccgagca gaccgtcgcc gtgcttgagc gtaccggcgg attcctttgc cacaaaccga
    23281 gccgcgccgc aaccacccat attcttgtct tcgtagccgc tgtcgccgtt gcctgccacc
    23341 gtgctgatga tgccggttat cgcatcaacc ttgcgcacgc gatgattcat cgtatcggag
    23401 acgtacagat tatcctgctc gtcgaacacc acggcctccg gagcatggag catcgcacgg
    23461 accgccggct tctcgtcgcc gtcgtaaccg tagcggcaca ctccggccag agtcgagata
    23521 ataccgctct cctgatcgat ctttcgcacc cgattactgc ccttatcgac aaaaacgatg
    23581 ttgcctttac tgtccaccgc aatcccgacg acgtcgtaga gacgcgcctc cagagccggt
    23641 cttccatcgt ccaggtacat ggacatgtcc aggccgtccg agcagaccgg catcaaccag
    23701 ccgtgatcgt cgctgaaatc gatgccaata gcatcgccgg agagcaccac cagattgcgc
    23761 gccacaagcg gctgttcgcg agcgtcatcc tcggccgtcc aggttccggc aatcgtcgac
    23821 accataccgg tgcgtggatc gtagcggcgg atcctgtggg cctgcgtatc agcgatgtac
    23881 atgacatcgt ctttgtcaac agcgatcgcc gctggccatg tcaggttgac ttctagagca
    23941 gggccgtctc cgttaaaccc atgctgcccg gtacccacca ccgtggtgat caccccggta
    24001 tcccggtcaa cccttctgat gcgattgctg ccggagtcgc agatgtacag gtggttgtgg
    24061 gaatcgaagg caacgtcaag cggcagatac aagcccgctt caccgcaggg tccctcgtcg
    24121 ccgctgtaac aggtttcgcc gattccggca aagttgtgca gggttccctc ttgaagattc
    24181 acacggcgga tacgatcgga ccccgactcc gcaaagtaaa gccagtgctc ttctttgtcg
    24241 agggccgcgt ggtgcggcaa gggaatgccg gctttcacgg cccgtttgcc gtcaccggtg
    24301 ctgcgggctt ttccgttccc ggcaaaggtc tcgatgtatc ccagtgcgag ctgaagttcc
    24361 gtttccatat tgtaatggtc gatcctgccg cttaggcgcg ggtggccggc tgtgccgccg
    24421 gttgttccac aacgggagcc gcctgggtcg gcgttgccgg aatcgcctgc tgaggtgtcg
    24481 ccgtttgctg ttgagcggcc tcggcttcgg cttcgatggc atccgcttca tgcacggact
    24541 tcttaaatcc cttgatcgcc ttgccgattc cttctccaag ctgcggcagt ttgccggcgc
    24601 cgaagatgat cagcacgata aagaggatga ggatcagctc tgtaaaacct agactcccaa
    24661 acatggtgtg ttcctccgct aggggtgaat caggatccgc tgctttcctt gctccgtttg
    24721 gcaaaccgtt ctttcaaccg ctgcctcgcc tgttcagcct gctcgaacat cttgcacttg
    24781 tcgtacacgc tgatcagatt gtagtaggcc aatggctcgt cgggactctg ctcgacggca
    24841 tcctccatga ccttcatggc caggtccgtt tttttgtggt tcaacgcgag ttccatcaat
    24901 ttaaaactgg cttccagatg cttcgggtcg agttccaggg tacgcaggta gcactgaatc
    24961 gccatgtcga tcgtgttgta atccgagacc tggggattgt cgagttcaac gtagacgccg
    25021 gccaaattgt accaggcgat ggggtcttcc ggcgtgatct cgaccaggcg ctcgtagtag
    25081 cctttggctt ccatgtaatt ctttttgtcg gcatagacgc ggccgaggtt gaagagcgcc
    25141 aagacgtcgt attggaagac ctcgagggcg tgcttgaact ccgcctcggc ttcgtccgtc
    25201 atgttcttgg tcgcatagat ggtcccaagg ttcgaataat acatggcgag cgagcggttc
    25261 atttcggcgc gaagcccttc cgccatctcg atagatttct tgacctcaac cagcgcatcg
    25321 tccagccggc ccttgctgaa gtagagctcg cccaaacggc aacgggcttg aaaatcatcg
    25381 ggctcggtcg cgagcaactt ctcgatctcg gcgatctctt cgtccggatt cagcggcaga
    25441 tcgcccggat ccacggcagc gcggccttgt tcgggagcgg aagcggttgg ttcattcatg
    25501 ggcaagtcct atcaaaaagt gtttccgtta cacgtggccg ccgaccagcg agtccggggc
    25561 ccccatcacc gtcgcactcg aggctggcac ctgatctggt gcgtcacgcc gatagcgatc
    25621 catcgtcagc agcatgacgc caagaacgac catgagcgtc aagttcgaat acaacaacgc
    25681 atagcctgcg ataaacatga gccccagccc atagccggcc aatcgcccca tcgtcaggag
    25741 cttgatcaca gtataggacc aacagattaa gccagccatg tagagcgcaa agggtaagta
    25801 gaacgtgtat cccatcccga actggaagat ccagccgatc ccgtcggcac ctttccgcac
    25861 gttgttcgcc aaagctgggt catactggtt caagagatag tccaggaaga acatggcggt
    25921 gaaaatcgca gccgctgtac cccagaacca gagcgcccgt cggcgctgcc gttgattgcg
    25981 tgagcggaac gagacgccac ggccgtaggc ccaaaacacc agaatactgg ctaagaccat
    26041 cagcgcttcg cctccccgat gtgattcgta gaccatgggg ggcgcagcca ccgtaccggc
    26101 gaaactgtaa aaggtcgaca cgatctgata gtacaaccag ccggagatcc ccagcaggta
    26161 cgtcaggccg agcgtccgat gagaccaatc ctgatgcgtt atcaaatatt ccgccatcaa
    26221 gagcacgatc gcgaccaagg ccacggtgtt gtatatcccg gagcccagca tggtcggagg
    26281 cacgaggaga aatccgatcg tcaacaagag gagcaacccg acgcagggaa tcgaaaaccg
    26341 gttgaggccg gtcaaccccc gactcgccag tcgattggcc ataccgacgc tcagccccag
    26401 aaagagcaga atggcaacca cattcaacag ccactgcccg atttccgtga gcgcggtaaa
    26461 ggtcggaata atccactgat ggtccgccgc aagcttgctc agatgcatcc ccagacgcga
    26521 gaccaagcgg tacaggacga gttcgaggaa tgcgaccagc aggaccagct tgatcgtgta
    26581 ttggaacagc aggggctgat cactcagctt tcccgttagc cgttcgatag tcgttcccgg
    26641 tgccagcacg cagctactcc cttaatagtc ggaactgtag attatacaga tcgggcaaaa
    26701 taccggtcaa cgccgcgcca aggcgcagcc ctacaattga gcggcgggaa ctgcaccccg
    26761 ctcttcggcc tttgcccgtg cgatatacag caatccatcc gccattgaat agttgaacgg
    26821 cagctcgcac acgacctcac tgaccttttc gtacacatgc tgatacaacc gttccgcctg
    26881 atccggcgtc atgccttcct gcacttcata gaagaaaccg aggctcaagt cctccgccga
    26941 cggccgcatg atgcgcctga tcccatagcc gcccggatcg ctcatgatcg gcgcttcctt
    27001 ttccagatca aacaggcagg gctggcacga gaacccgtag gactcgttca gtttcttgtt
    27061 ctcgacgata aaattcatcg tctccagcgc ttcctcttcc ttttccgttg ggaagccgac
    27121 gatgatatag caatggacgg cgatcccgag atccacgcaa tcgttcgaaa tccgtctgac
    27181 ccattcctgc tggatgcctt ttttcatgaa atccatgacg cgctgattga aagactcgag
    27241 cccgaatacg atcttcaagc agccggcgtc gcgcagggag gtcagcagct cgcgagttaa
    27301 atgtttttca aagcgcatct cacaggtcca cctgatgtcc aaatgacggt cgatcatctg
    27361 ctggcagagc cgctttgttg gagagagcgc gaaacattca tcggtgaaga agaagttgcg
    27421 cacaccatac cgttgcttga gccattccaa ttcctcgatc gtccgtcccg gctctttctg
    27481 ccggaagttc tggtgatcga gcgtcagcgc gcagaacgcg cagtccttgt aataacatcc
    27541 gcgtgaaaat tgaacgggca gcacgggctc cggcgacaga tagcggccga gcgggaaccc
    27601 gtcgtaattc ggcgccggca gctggttgac gttttccgag tagaaaggct ggttgacgtt
    27661 gatcttgccg ttctggcggt aaatgagatt cggaacctta ccgaaatctt tcttccctgc
    27721 catttgattg accagttcca acaacgccgt ttcaccttca aagaccacga tgtcgtcggt
    27781 caagtcgaac aggctggggc accggcgcaa gttatcaacc aggcgcgtaa aaatgctccc
    27841 cccgatcgtc agatggatgt cgggcgcggc ctctttgatc aaacggcaga gagtgagccc
    27901 cgggatgatc tgcgaagtcg ccgtgatcga cacgccgatc aactctggac ggtctccgat
    27961 aatcgagggc agaaaccgct cgcggaacag cctgatataa ggattttgct gctcgtcccg
    28021 gatgacctgc atgagatgct tggacgagta aatggaatag gtgctgaatt gattgtccac
    28081 gaccgtcaat ctggtcggga aatagaccga ggagatgact tccagccact tgtcgatcaa
    28141 gaagagactt tcccgatagg cctccatatc gtagaagcct tcgcctctca gcgtctcctt
    28201 ggccaattcg atgcgatcaa tcagataggg gaagcgatcc agagactcga ccactcgcgc
    28261 caaatgctct gcgctgccag gaccggtttc gcctgtccgc tcccgttcca gaaacttttg
    28321 cttgtcgacg agttcctggt agaggccgac gccgtacgac ttggttacga tttcgtcaag
    28381 cagttcgatt cccagatctc gctgggagac attggtcacc ccagcctgag ccagaaatcc
    28441 cgtgagcgaa ggaagactca agtagggctg ggagggatgc caggtcgggg gaaagaggag
    28501 tgaaactttc atacggattc tctaccgaga gaattgaaaa ataagataaa ttctcgaaag
    28561 atcccgagtc tgttttatac actccgtacc ggcgcaaaag caaccctgca ccggtagcct
    28621 atttccgatg gtgtctgatc gtcctgtgac atccaccgcg cctgtctcct ggctgaaaaa
    28681 gaaaggcccc agttcggaga actggggcct ttctttacct tatacgttcc actcactaag
    28741 ggtggaacat cggctttctc gaccgatcag cttacggaag cttgagcgtg aaccaggtgg
    28801 agagcgcctt catgccgtta cgctcaatgt tggaaccgtc ccacaccgca aaggcgatag
    28861 gcacggacat cccgcccttg aactgcgtgt cgttcgcatc tcccgtttcc agggtgcgct
    28921 tcacgacgac gcgccaggtc ggaccggagt aaccaccacc cttaactgaa cctgacggtt
    28981 cccacacgcc gttgccgatc acatcctggt gagcctgcgt cgtcagggtg ctgaagccgt
    29041 tggcgttcag atcctcgacc gagctcacac ggagggtcgg atcagacatg atgttacccg
    29101 accaaatgcc ggggttgaag ggcccaaggc tgcgaccgat ccggtccgga taggtcacac
    29161 cgcccgcggg ctcttcgaaa tagtagtccc agaaaatgcc gggatattga tcgtccacgt
    29221 cccagatccc ggcgctg
//
LOCUS       DATIFR010000030        10208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_399812, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000030 DATIFR010000000
VERSION     DATIFR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399812"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VLD60_01530"
     CDS             <1..280
                     /locus_tag="VLD60_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HSB43660.1"
                     /translation="PEEVQGGTFTITNHGGFGSLFSTPIIHQPQVAILGVGAIQKRAV
                     VVNDAIAIRPMGYLSLSFDHRVIDGATADQFMARLKGYLEQSNWEHIL"
     gene            322..1521
                     /locus_tag="VLD60_01535"
     CDS             322..1521
                     /locus_tag="VLD60_01535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSB43661.1"
                     /translation="MNGTKQAVIVSAVRTPMGSFNGMFSAVPATKLGSIAIAEALKRI
                     HVAPERVDDVYMGSVLSAGLGQAPARQASIGAGIPNSVGATTVNKVCGSSLQTVIMAS
                     HAIALGEARIVVAGGMENMTRAPYLLEKARQGYRLGHAELVDSVVKDGLWDVYNNFHM
                     GHGGELCAAKYRLTREELDDFALESYRRAQHAIATGAFKPEIVPVDVPQRKGAPLTVT
                     DDEEPHRVDLSKLRHLKPAFQEDGLLTVGNSPSCNDGAAALVVMAEEEATHLGLAPMA
                     RIVGYAGAALAPEWFTIAPVEAIKLVLKKTGLSLGDIDLFEINEAFSAVSLTINRELG
                     LDPKKVNVNGGAVALGHPIGATGARILTTLLYAMEAQGVRRGVASLCIGGGEALAIIV
                     ERQGVRG"
     gene            1586..2485
                     /locus_tag="VLD60_01540"
     CDS             1586..2485
                     /locus_tag="VLD60_01540"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HSB43662.1"
                     /translation="MRLDDIKTIGIVGAGQMGRGIAQVCATTGYRVQLVDVAEPSLTE
                     ALAKIRTGLERAVEKGSLTDHQAGEVLTLIHPLVQLDRLQDVQLVIEAIPEDLALKQE
                     LFAELNQICQPHTVLASNTSSISITKLGAASGRPDRVVGLHFMNPAPVMRLVEVVRGL
                     ETSERTMNLALDLAKQLGKTPVVAKDVPGFIVNRILIPMINEAVFTLEQGVASAEDID
                     VAMTIGANHPVGPLALADRIGLDTVLAICEVLYQNLGDSKFRPCPLLRRYVEAGWLGR
                     KTGHGFYVYEASGERHEATINKI"
     gene            2528..4123
                     /locus_tag="VLD60_01545"
     CDS             2528..4123
                     /locus_tag="VLD60_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSB43663.1"
                     /translation="MQERKPRFTSLSGLDIDRVYTRDDLKDWSPEDDLGSPGEFPYTR
                     GVYPTMYRGRLWTMRQFAGFGSAQDTNRRFKYLLQHGQTGLSVAFDMPTLMGIDADDP
                     RAHGEVGHCGVAISSLADMEQLFEDIPLDRVTTSMTINGPAAVIFAMYLAVAEKRGIA
                     VESLGGTLQNDILKEYIAQKEWLFPPEPSLGLMTDTIAYCAKQVPKWHPVSVSGYHIR
                     EAGSTAVQELAFTLYDGLTYVEAAVKSGLAVDTFAPQLSFFFNVHNDFFEEIAKFRAG
                     RRIWAREMTRRYQPKDPRSSQLRCHAQTAGCSLTAQQPMNNVVRTTLQALAAVLGGTQ
                     SLHTNSMDETLALPSEEAVKVALRTQQLIAQESEVTNTVDPLGGSYYLETLTNRLEEA
                     VLDFFRKLDDLGGMVKAIERGFPQREILDASQRYQREVERKERIVVGVNDYLEEDSRP
                     IPILKIGPEVEREQVTRLSDLRKSRDSFRMAGALEELQEAASCGENVMPYLIEAVKAK
                     ATLGEICAALKEVFGTYREPVVL"
     gene            4223..5071
                     /locus_tag="VLD60_01550"
     CDS             4223..5071
                     /locus_tag="VLD60_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB43664.1"
                     /translation="MKLGALDIYPVTDGRFRLDGGAMFGIVPKVLWQACCEADELNRI
                     PLSLTCLLIRAQGKNILVDTGLGSKEDGKFQRMFAVERTQTLLDSLKRLDVGPEDIHI
                     VINTHLHFDHAGGNTMKQENGTIMPTFPKAQYFIQHGEFEDASRANERTKGSYRRDNF
                     TPIAHVNRWEFLHGDTELLPGITAVVTAGHTRCHQGVKIESEGKIAFFLGDLIPTVSH
                     LPLPYIMGYDLFPIQTLETKRWVLDRAFEERWLLLFEHDPLIQAGDVGKDLEGKYFLR
                     EVKACQ"
     gene            5062..5469
                     /locus_tag="VLD60_01555"
     CDS             5062..5469
                     /locus_tag="VLD60_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSB43665.1"
                     /translation="MPVAAPPLRVLIGKVGLDGHDRGVKLIARALRDAGVEVIYTGLH
                     QTPEQVVSTAIQEDVDAIGLSILSGAHNTLFRRVVELLKEQSVEDIVLFGGGIIPDED
                     LPALKSAGVKALFRPGAPMNEIVDFVRGLKARG"
     gene            5476..7080
                     /locus_tag="VLD60_01560"
     CDS             5476..7080
                     /locus_tag="VLD60_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSB43666.1"
                     /translation="MHILKTSLVGSSEEFTSNRAHYEGLRADLQKHLVLVRAGGRDEA
                     VALHKQRGKLTARERIEALRDPDSPWLELSPLAACGMYDDQVPAAGLISGVGYISGRP
                     CVIAANDATVKGGTYFPMTIKKHLRAQDIALENRLPAIYLVDSGGVFLPMQAEVFADK
                     DHFGRIFYNQARMSALGIPQIAVVMGMCTAGGAYVPAMCDENVIVKGTGTIYLGGPPL
                     VKAATGEDVTPEELGGADLHTRLSGVSDHLAENDLDALEICRSIVATLPRRPARRREG
                     EIEDPLYQASDLYGLIPSNPRKTFEVREIIARLVDGSRFHEFKARYGQTLTCGFARWM
                     GYQVGIVANNGVLLSEAALKGAHFVQLCTQRRIPLVFLQNITGFMVGKDYEARGIIKD
                     GAKMVQAVATADVPKFTVIIGASHGAGNYAMCGRAYGPRFVFLWPNARISVMGAQQAS
                     HVLVTVKRQQRAREKEMLSEEEQRKISESTLSQYEREGSPYFSTARLWDDGIIDPTDT
                     RHVIGLCLDIAMTTPVRGSHPPVFRM"
     gene            7088..7939
                     /locus_tag="VLD60_01565"
     CDS             7088..7939
                     /locus_tag="VLD60_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSB43667.1"
                     /translation="MKDRVAILVESSNGLACVTLNRPHRRNAFDASMVGELCEVFDEL
                     GRNPSIQVIVLAGTGSAFCAGVDLNWVGSDGAVSAPEAQKDAEQLREMFRAIDECPCP
                     VIARIHGAAFGGGVGLIAVSDIAVAVADATFAFSEVRLGLVPAVITPFLLRKTGESFL
                     RRFCLTGEPFSASAAQQCHLVHDVVERDKLDSRVDELVNMILRLAPQAIRDTKVLFRR
                     LPALSENERWRACIEANVHARLSPEAQEGLRAFLERRPPVWTDLSGARGKEPREGKPQ
                     NVAGQQT"
     gene            7917..8861
                     /locus_tag="VLD60_01570"
     CDS             7917..8861
                     /locus_tag="VLD60_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HSB43668.1"
                     /translation="MSQGSRHRTTSRPIRIIEVGPRDGLQNEPDIVSTEDKVSFVNAL
                     SGTGVAEIEIGSFVSPQAIPQLADSDKVFRKIERRPGVTYSALVPNVRGFERARAVAM
                     EKIAVFTAASETFARHNIKCGICESIDRFAPVVDAAKREGMTVRGYISTVTHCPFEGL
                     VDPSHVVEVIQRLLQIGVDEISLGDTVGKATPSDVRRLLDEILPHIERSRLSLHFHDT
                     YGMAVANVLAAWMDYGIEAFDTSAGGLGGCPYAPGASGNVATEDVVYALKASGAAVLV
                     DEQKVIAAARQIGKVLNHPLSSRLSHIQTEQTAYARLT"
     gene            8858..9661
                     /gene="meaB"
                     /locus_tag="VLD60_01575"
     CDS             8858..9661
                     /gene="meaB"
                     /locus_tag="VLD60_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSB43669.1"
                     /translation="MTASNPHLSIRDNAGVMNLAELVRAGNVRAVSRVITLLENRDPD
                     GSAALGLILLGSHRAAVIGITGYPGAGKSTVVDQLVAAYRRLGMKVGVLAVDTSSPVT
                     GGALLGDRIRMQEHTLDHGVYIRSMATRGHHGGLAQASRDATLVLEAAGYEVILIETI
                     GVGQNEMDVVEIAQTVVAIVAPGLGDEVQAMKAGLLEVAHIVVVNKGDRAGADTTLRD
                     LRDWCPTVLRTVATTGEGIPGLVTAIAEHQRLHDLQGSPARPLQEGTGF"
     gene            9678..>10208
                     /locus_tag="VLD60_01580"
     CDS             9678..>10208
                     /locus_tag="VLD60_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSB43670.1"
                     /translation="MPHQLLTISHHVARIMLHNPPANVLNLSVFKELEQVLNEVEEDE
                     YVRGVIVTGSGRFFCAGADMNELAHLNTVHSGSEFAVRGQSLLSRIERSDKPILAAIN
                     GTCVGGGLELALACHIRVAAAGAMLGLPEIKLGLIPGFGGTQRLPRVVGASKAAEMIL
                     TGESVSAEEAFRMGLVS"
BASE COUNT         2126 a         2864 c         3195 g         2023 t
ORIGIN      
        1 tcccgaggag gtgcagggag ggacattcac gatcacgaac cacggtggat tcggcagcct
       61 cttcagcacg ccgatcattc accagccgca ggttgccata ctgggggtcg gagcgatcca
      121 gaagcgggcc gtggtcgtca atgatgccat cgccatccgg ccgatgggct acttgagcct
      181 gtccttcgat caccgggtca ttgacggggc gacggcggat cagttcatgg ccaggctcaa
      241 gggttatctc gaacagagca actgggagca catcctgtga acgggaccgc gtgaggggtg
      301 aggagtggag gtgagagagc tatgaacggg acgaagcagg cggtcatcgt cagcgctgtg
      361 cgcacgccga tggggagctt caacgggatg ttcagcgccg tgcccgccac caagttgggc
      421 agcatcgcca ttgctgaagc gctgaagcga attcacgtcg cgccggaacg cgtggacgac
      481 gtgtatatgg gctctgtgct cagcgccggg ctcggtcagg cgccggccag acaagcgtcg
      541 atcggagccg gtatccccaa ctcggtcggc gctacgaccg tcaacaaggt ctgcgggtcc
      601 agtctccaga cggtgatcat ggcctcccat gccatcgcgc tcggtgaggc gaggatcgtc
      661 gtggcaggcg gcatggaaaa catgacccgc gcgccctatt tgttggagaa ggcgcggcag
      721 ggatatcgac tgggacatgc agagttggtg gacagtgtcg tcaaagacgg cctgtgggat
      781 gtctacaaca attttcacat gggccatggc ggtgaactct gtgccgcgaa gtaccggctc
      841 accagggagg aactcgacga tttcgccttg gaaagttacc gtcgcgcgca gcatgccatc
      901 gcgacagggg ccttcaagcc ggagatcgtg ccggtggacg tgccacaacg gaaaggcgcg
      961 cccttgaccg ttacagacga tgaagaaccg catcgagtcg atttgagcaa gctgcgccac
     1021 ctcaagccgg cattccagga agacggcctc ctgaccgtgg gtaattctcc ctcttgtaac
     1081 gacggggcgg ctgccttggt ggtgatggct gaagaggagg ccacgcatct cgggctcgcc
     1141 ccgatggcgc gcatcgtcgg ctatgccgga gcggcactgg cacctgaatg gttcacgatt
     1201 gcgccagtcg aggcaatcaa gctggtgctc aagaaaaccg gcctctcact tggcgacatc
     1261 gacctgtttg agatcaatga agccttctcc gccgtctcgc ttacgatcaa ccgggagctc
     1321 gggcttgatc ccaagaaagt taatgtcaac gggggcgcgg tcgcactggg tcatccgatc
     1381 ggcgctaccg gcgcgcgtat tctcacgacg ctgctctatg ccatggaagc ccagggcgtc
     1441 aggcgagggg tagccagtct ttgcatcggg ggtggcgaag cattggcaat catcgtggaa
     1501 aggcaagggg taagaggcta gaggcaatgc gcgagatttg aaatacctct agcccctcgc
     1561 ctcgtgcttc tggcccaagg tagttatgag actcgatgac atcaagacga tcggcatcgt
     1621 cggcgccggc cagatgggcc gcggcattgc ccaggtgtgc gcgacgaccg gctatcgtgt
     1681 gcagctcgtc gatgtcgcgg agccgtcgtt aacggaggca cttgccaaga tccgtaccgg
     1741 cctggagcga gccgtggaga agggaagtct caccgaccac caggcgggtg aggtactgac
     1801 gctcatccat ccgctcgtac agctcgaccg gttgcaagac gtgcagctgg ttatcgaggc
     1861 gattcctgaa gacctcgccc tcaagcagga actcttcgcc gaattgaacc agatctgcca
     1921 gccgcacacc gtcctcgcca gcaatacctc gtcgatttcg atcacgaaac tgggcgcagc
     1981 ctccggtcgg cccgatcgag tggtgggcct gcacttcatg aatccagccc ctgtgatgcg
     2041 actcgtagaa gttgtccgcg gtctcgaaac atccgagcga acgatgaatc tggcacttga
     2101 tctggcgaag cagctgggga agacccctgt ggtggccaaa gatgtgccgg ggttcatcgt
     2161 gaaccgcatc ctgattccca tgatcaatga agctgtattc acgttggagc aaggtgtcgc
     2221 gtcagctgaa gatattgatg tggcgatgac gatcggggcg aatcatccgg tcggaccact
     2281 ggcccttgcc gaccggatcg ggctggatac cgtactcgcc atctgcgaag ttctgtacca
     2341 gaacttgggc gattcgaagt tccgcccctg cccgttgctc cgccggtatg tcgaagcagg
     2401 gtggctggga cgcaagaccg gtcatgggtt ctatgtgtat gaggcaagcg gcgagaggca
     2461 cgaggcgacg atcaacaaga tttgaacctc tatcctgtcg cgcgtttcac gccggaggta
     2521 ccgatcgatg caagaacgga aacctcggtt cacctcgctc tcaggactcg acatcgatcg
     2581 cgtttatacg cgtgatgatc tgaaggactg gtcgcctgag gatgatctcg gttcgccagg
     2641 cgaattcccc tatacgcgcg gcgtctatcc caccatgtat cggggccgcc tctggacgat
     2701 gcggcagttt gctggattcg gatccgccca ggatacgaat cgccgattca agtatctcct
     2761 ccagcatggg cagaccggcc tcagcgttgc cttcgatatg ccgacgttga tgggaatcga
     2821 tgccgatgat ccgcgcgccc atggcgaagt cggtcactgc ggcgtggcga tctcatcact
     2881 tgccgatatg gagcagctct ttgaggatat cccgttggat cgggtcacca cctcgatgac
     2941 gatcaacggg ccggccgccg tgatctttgc catgtatctg gcggttgcag agaagcgcgg
     3001 catcgcagtt gagagcctag gcggcacgct acagaacgac attctgaagg aatatatcgc
     3061 gcagaaggaa tggctcttcc cgccggaacc ctcgctgggc ctcatgaccg atacgatcgc
     3121 ctactgtgcg aagcaggttc ctaagtggca tccggtcagt gtcagcggct atcacattcg
     3181 cgaggcggga tcgactgcgg tccaggaact ggcctttacg ctctatgacg gactgactta
     3241 cgtcgaggca gccgtgaaat ccggtcttgc ggtggacacc tttgcccccc aactgtcgtt
     3301 cttcttcaac gttcataacg acttctttga agagattgcc aagttccggg ctggccgccg
     3361 gatctgggca cgcgaaatga cgcggcgcta tcagccgaaa gatccgcgat cctcgcagct
     3421 ccgctgccac gcccagacag ccggctgctc actcaccgcg cagcagccaa tgaacaatgt
     3481 ggtccgcacc acgttgcagg cgctggcggc cgtgctggga gggacacaat ctctccatac
     3541 caattccatg gacgaaacgc tcgccttacc ctccgaagaa gcggtgaagg tcgcgttgcg
     3601 cacccaacaa ctcattgccc aggagagtga agtaacgaac accgtggacc cactgggcgg
     3661 atcgtactat ctcgaaactc tgacgaatcg actggaagaa gcggtgctcg acttcttccg
     3721 caagctggac gacctgggtg gcatggtgaa ggcaatcgaa cgaggcttcc cgcagcggga
     3781 gatcctcgat gcttcacagc gctatcagcg cgaagtggag cgaaaagagc ggatcgtggt
     3841 cggggtcaac gactacctgg aggaggactc tcgcccgatc cctatcctca agatcgggcc
     3901 tgaagtggag cgcgagcagg tgacgcgtct ctctgatctg cgcaaatcgc gcgattcgtt
     3961 caggatggcc ggcgcactcg aggaattgca ggaagcggcc tcctgtggcg aaaacgtgat
     4021 gccctatctg atcgaggcgg tgaaggcgaa agcgacgctg ggcgagattt gtgcagcgtt
     4081 gaaagaagtc ttcggaacat acagggagcc ggtggtgcta tgagagtaat cagcagtcag
     4141 cgatcagcta tgagcatgaa gcagtcgctc gggaagacct agctgaacgc tgagggctga
     4201 aagctgaatg ctcggtgaaa gcatgaaact aggagcgctt gacatctatc ccgtgaccga
     4261 cggtcgattc cgtttggacg gcggcgctat gttcggaatc gtgccaaaag tcctctggca
     4321 ggcgtgctgt gaagctgacg aactgaatcg catcccgttg agtctcacct gtctgctgat
     4381 ccgggcgcag gggaagaaca tccttgtgga tacgggactg ggctccaagg aagacggaaa
     4441 gtttcagcgg atgtttgccg ttgagcgaac acagacgtta ctggactctt tgaagcgtct
     4501 agacgtgggt cctgaggata tccacatcgt catcaacacc catctgcatt ttgatcatgc
     4561 aggcgggaac acgatgaaac aagagaacgg gacgatcatg cccacctttc ccaaagccca
     4621 gtatttcatt cagcacggtg agtttgaaga tgcctcccgc gccaatgaac ggaccaaagg
     4681 cagctatcgg cgagataact tcacaccgat cgcccacgtg aatcgctggg aattcctgca
     4741 tggtgatacg gaactactcc ccggaatcac cgccgtcgtc accgcgggcc atacgcgctg
     4801 tcaccaagga gtcaagattg agtcggaagg aaagattgct ttcttcctgg gcgatctgat
     4861 ccccaccgtg tctcatttgc cgcttcctta cattatgggc tacgacctgt tccctatcca
     4921 gacgctggag acaaaacgat gggtcttgga ccgagccttc gaagagcgat ggttgctgct
     4981 cttcgagcac gacccgctga ttcaggcagg tgatgtcggg aaggatctgg agggcaagta
     5041 ctttctccgt gaggtgaaag catgccagta gcagcaccac cccttcgtgt gttgatcggc
     5101 aaagtggggc tcgacggcca cgaccgtgga gtaaaactga ttgcgcgcgc gttgcgtgac
     5161 gccggggtgg aagtgatcta taccggcctc caccagacgc cggagcaggt cgtcagcaca
     5221 gcgattcaag aagatgtcga tgcgatcggc ttgagcatcc tctccggtgc gcacaatacg
     5281 ctcttccggc gcgtcgtaga gctgctcaaa gaacagagcg tcgaagacat tgtgttgttc
     5341 ggcggtggga tcattccgga cgaagatctt cccgcactca aatcggccgg cgtgaaggcc
     5401 ctgttcaggc caggggctcc tatgaatgag attgtggact tcgtgagagg gcttaaagcg
     5461 cggggttgaa acatcatgca catcctcaag acctctctcg ttggatcatc cgaagaattc
     5521 acatcgaatc gggctcacta cgaaggcctg agagccgatc tccaaaagca tctcgtcctc
     5581 gttagggctg gtgggcgcga cgaggccgtt gcgctccaca aacaacgcgg caagctgact
     5641 gctcgcgagc ggatcgaggc gttgcgcgat ccggattccc catggctcga gctcagtccc
     5701 ctggccgcct gcggcatgta cgacgaccag gtccccgctg caggcttgat cagcggcgtc
     5761 ggctacatat cgggacgacc ctgcgtcatc gcagccaacg acgccacagt caaaggcggc
     5821 acttacttcc cgatgacaat caagaaacat ctgcgggcgc aggacattgc cctggagaac
     5881 cggctgcccg cgatctatct cgttgactcc ggcggggtgt tcctgcccat gcaggccgag
     5941 gtatttgcgg ataaggacca ttttggacgg atcttctaca accaggcgcg gatgtcggcg
     6001 cttggcattc cgcagatagc cgtggtgatg ggcatgtgca ccgcaggcgg cgcctacgtg
     6061 ccggcgatgt gcgacgagaa tgtcatcgtg aaaggaaccg gcaccattta tttgggcgga
     6121 ccgccgcttg tgaaggctgc gaccggggag gatgtcacac ctgaagagct cggcggcgct
     6181 gatttgcata cgcggctgtc cggcgtgagc gaccatctgg ccgagaatga tctggatgcg
     6241 ttggagatct gtcggtcgat cgtcgccacg ttgccccggc ggcctgccag acggcgtgaa
     6301 ggtgagatcg aggatccgct ctatcaggcc agcgacctct atggactcat cccgagcaac
     6361 ccacggaaaa cctttgaggt gcgcgaaatc attgcccgcc tggtggacgg aagcagattt
     6421 cacgagttca aggcacgcta cggccaaacg ctgacatgcg ggttcgcccg ctggatgggg
     6481 tatcaggtcg gcatcgtcgc caataatggc gtattgctct cggaagccgc attgaaaggg
     6541 gcacatttcg ttcaactctg tacccaacga cgaatcccgc tcgtctttct gcagaatatt
     6601 actgggttca tggtcggcaa agattatgag gcccgcggga tcatcaagga cggtgccaag
     6661 atggtgcagg cagtggcgac cgccgatgtc ccgaagttca ccgtcatcat cggcgcctct
     6721 cacggtgccg gcaattatgc gatgtgcggc cgtgcatatg ggccgcgatt cgtattcctc
     6781 tggcccaacg cgaggatttc ggtgatgggg gcgcagcagg catcccatgt gctggtgacc
     6841 gtcaagcgcc agcagcgagc gcgggaaaag gaaatgctat ccgaggagga gcagcggaag
     6901 attagcgagt ccaccttgtc ccaatatgag cgggaaggca gcccctattt cagtacggcc
     6961 cgcctctggg atgacggcat catagacccc actgacacgc ggcacgtcat cggcctgtgt
     7021 ctcgatattg cgatgacaac gccggtccgg gggtcgcacc cgccggtgtt tcgaatgtga
     7081 ggaatgcatg aaggaccgtg tcgcgatcct ggtcgagtcg agcaacggcc tagcctgtgt
     7141 gacactgaat cggcctcatc ggcgaaacgc gtttgatgcc agcatggtcg gagaattgtg
     7201 cgaggtgttt gacgagttgg gtaggaaccc ttcgatacaa gtaatcgtct tggctggcac
     7261 tggttccgcg ttttgcgccg gtgtagacct taactgggtg gggtcggatg gcgctgtgtc
     7321 cgctcctgag gcgcagaagg atgctgaaca gctgagggag atgttccgtg ccatcgatga
     7381 atgtccctgc cccgtgatcg cgcgaatcca tggggcagct ttcggaggcg gagtcgggct
     7441 gatcgcagtc agtgacattg ccgttgcagt ggcggacgcg accttcgcct tcagtgaggt
     7501 ccgattgggg ttggttcccg ctgtgattac ccctttccta cttcgcaaga ccggtgaatc
     7561 gttcctgaga agattctgcc tgaccggcga gccattttcc gcatcggctg cccaacagtg
     7621 ccacctcgtt cacgatgtgg ttgagcggga caagctcgac tcccgggtcg atgagttggt
     7681 gaacatgatc ttgcgtctcg cgccacaggc gatacgggac acgaaggtcc tgttccggcg
     7741 acttcccgcc ctctccgaga acgagcgatg gagagcctgt atcgaagcca acgtccatgc
     7801 ccgtctttcg cccgaggcac aagagggact ccgagctttt cttgaaaggc gcccccctgt
     7861 ttggacggac ctgtcaggag cacgagggaa ggaaccccga gaagggaaac ctcagaatgt
     7921 cgcagggcag cagacataga acgacgtcgc ggccgatccg catcatcgag gtcggtccac
     7981 gggacggact ccagaatgaa ccggacatcg tctcgaccga agacaaggtg tcgtttgtga
     8041 acgccctgtc aggaaccggt gtggcggaaa ttgagatcgg ttcgttcgtg tcgccccagg
     8101 cgatcccaca actagcagac tcggacaaag tgtttcgcaa gatcgagcgc cggcccggcg
     8161 tcacctattc cgccttggtg cccaatgtgc ggggattcga acgggccagg gccgtggcga
     8221 tggaaaagat cgcggtcttc actgcggcct cggagacgtt tgcccgacac aacatcaagt
     8281 gcgggatatg cgagtcgatc gaccggttcg caccggtggt ggacgcagcg aaacgggagg
     8341 gaatgaccgt ccgcgggtac atttccaccg tgactcactg cccctttgaa ggtctcgtag
     8401 atccgtccca cgtcgtcgag gtcatacagc gccttctgca aatcggagtc gatgaaattt
     8461 cgctcggcga caccgtcggg aaagccactc cgagcgacgt gcggagactg ctggatgaaa
     8521 tattgccgca cattgagcga agccgtctct cactgcactt tcatgacact tacggcatgg
     8581 ccgtggccaa tgttctggcg gcctggatgg actatggcat cgaggcgttt gacacgtctg
     8641 ccggcgggct aggtggctgt ccctatgcgc cgggtgcatc gggtaacgtg gcaaccgaag
     8701 acgtggtcta tgcgttgaag gcgtctggag cggccgttct ggtggatgag cagaaggtca
     8761 ttgcggctgc cagacagatc ggaaaggtgc tcaaccatcc gctctcctct cgactgtcgc
     8821 acatacagac cgaacagact gcgtatgcga gactgacatg actgcttcta atcctcacct
     8881 atccataaga gacaatgccg gcgtgatgaa cttggccgag ttggtcaggg ctggaaatgt
     8941 acgtgccgtc tctcgtgtga tcacgctctt ggagaatcgt gacccagacg gtagcgctgc
     9001 acttggcctg attctcttag gttcgcaccg tgcggcggtg atcgggatta ctgggtaccc
     9061 gggcgcgggc aagagcacgg tggtcgatca actcgtggca gcctatcgac gtttgggcat
     9121 gaaagtcgga gtgttggccg tcgacacgag cagcccggtc actggcggcg ccctgctcgg
     9181 cgatcgcatc aggatgcagg aacatacgct cgatcatggc gtgtatatca ggagtatggc
     9241 cacacgcgga caccacggag ggctcgccca agcgagcagg gacgccacac tcgtgttgga
     9301 ggcggccggg tacgaagtca tcctgatcga gaccatcgga gtcggtcaga atgagatgga
     9361 cgtcgttgaa atcgcccaga ccgttgtcgc aatcgtcgcc cccggcctgg gcgatgaggt
     9421 gcaggccatg aaggcggggt tgttggaagt ggcccacatt gtcgtggtca acaaaggcga
     9481 tcgtgcgggc gcggatacca cgttgcgaga cttgagagac tggtgtccga ccgtgctccg
     9541 gaccgtggct acgacagggg aagggattcc ggggctcgtc actgcaattg ccgagcatca
     9601 acgactgcac gatctccagg gctcgcccgc tcgtcctcta caggaaggaa ccggtttctg
     9661 aagagtagga ggatgttatg cctcaccaac tgttgacgat ctcccaccac gtcgcacgga
     9721 tcatgttgca taatccgcca gccaacgtgc tcaatctctc cgtgttcaag gaactggagc
     9781 aggtcctgaa tgaggtagag gaggacgagt atgtccgggg agtgattgtg accggttccg
     9841 gacggttttt ctgcgcgggg gccgacatga atgagctagc tcatctcaat accgtgcata
     9901 gcggctcgga gtttgctgtt cgcggtcagt cgctcctcag ccgtattgag cggtcggaca
     9961 aacccatcct cgctgccatc aacggtacct gcgtcggcgg cgggctggaa ctcgccttgg
    10021 cctgccacat tcgggtagcg gcagccggag cgatgctggg gctgccagaa atcaaactcg
    10081 gcctcattcc cgggttcggc ggcacccaac gactgccgcg agtcgtcggg gcttccaaag
    10141 cggcggagat gatcctgacc ggagagagcg tgtctgcaga ggaggcattc cgaatggggc
    10201 tggtcagt
//
LOCUS       DATIFR010000031         4605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1549239, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000031 DATIFR010000000
VERSION     DATIFR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549239"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VLD60_01585"
     CDS             complement(<1..759)
                     /locus_tag="VLD60_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB43671.1"
                     /translation="MAQYWQEISALTMRWVLRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADAQVTQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     SSVILSRFIFVMTITSLQVLVILGVAFLFGVQPATGLLGIATILLIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPEWMSFLARFNPMTWAIDAVR
                     PLILSNWSEALPHVGMVVVVMVIFDALCLYGGAKA"
     gene            complement(769..1740)
                     /locus_tag="VLD60_01590"
     CDS             complement(769..1740)
                     /locus_tag="VLD60_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB43672.1"
                     /translation="MDRAIAIDVSRLSKSYDGHVAVADISFQVYTGEIFGLLGPNGAG
                     KSTTLRTLITLLTPSSGTATVLGHNTVRDADKVRHLIGYVPQERAIDRFLTGKEHLEL
                     LAALYHLPKDEANKRIRELLKLVELEAHADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWDYVRMLKARGMTVVMTTNYLDEADQLCDRLAIIDGGTIKTI
                     GSPVELKVGLGGDIVSLTLKETDRIPALESALKGQPAIKAVRATEKGLDIRVESPEKA
                     LPVILDTANRLNCRVEFIQYNRPRLDDVFIAHTGRAITESVPPSETA"
     gene            complement(1740..2429)
                     /locus_tag="VLD60_01595"
     CDS             complement(1740..2429)
                     /locus_tag="VLD60_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSB43673.1"
                     /translation="MQTDREREHHEQDGHEKTTPFSIPATIPVFALPNVVLFPKTYLP
                     LHIFEPRYRQMVDDAVMGGQCIGMALLKEGWESSYYGNPPVFTMGCVGRLVSVQPLAD
                     GRSNILLQGMERFEISEESYDKPYRRASITLKTREAEARLDPAVRQRLVTALEEYLRS
                     RQEPPTWQSWFREDVSDDILVNTLSTYLDCTPLEKQFLLEADSFHQQARRLSDLIQFL
                     MHDHHGAKGWG"
     gene            2623..3735
                     /locus_tag="VLD60_01600"
     CDS             2623..3735
                     /locus_tag="VLD60_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HSB43674.1"
                     /translation="MRRSALLICMLLTVGTALAADIPHEFTPATEAYRYEFPRDHGSH
                     ETFRTEWWYYTGNLTSRDGRTFGYQLTFFRRAMPPDQVRTWPSKWSVSQLYLAHFAVS
                     DLDKKQFHYTEKLSRAGLGKAGAEANHLQVWIDDWRAESPSAQQNLHILKAAEGGLAI
                     QLELSPEKALVIHGTAGISRKGAEPGQASHYYSFTRMATAGRLTIGSTSFEVTGTSWM
                     DHEFGSADLGKDLVGWDWFSIQLDNRTEFMLYRLRRADGSADPASSGTLIDRDGQAYH
                     LSIGDFTLEPMSYWTSPANKARYPQHWRLTIPSKQVSLDLIPRLAEQELSTTRSTQVT
                     YWEGAIETTGTAQGQPIHGQGYMELTGYAERFAKKL"
     gene            3796..>4605
                     /locus_tag="VLD60_01605"
     CDS             3796..>4605
                     /locus_tag="VLD60_01605"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43675.1"
                     /translation="MLIRTIAQFASVALVLFCVGCQKDSEAIVSIALHPTNANILYVA
                     TNDAVYKSRDGGQFWEKFPSFSARRVTTVAIDPQLPATIYAGTMGDAVYKSPDGGQHW
                     LPHNVGLKEHVSFVNQFVFHPALSEKIYAATTVGAYYTKNAGREWEERMNGMKEVHIV
                     TSIAINSKDPTILYGGTTGGIYRSDDAAMSWKKINNGLIPDTELMASMALGVNAIEID
                     RVNSEIVYAGTTKGLFRTANRGEQWERIGLSLPDPFVSSIVIHPTEPSALYI"
BASE COUNT         1050 a         1380 c         1267 g          908 t
ORIGIN      
        1 cgctttagcg ccgccataga gacagagcgc gtcgaagatc accatcacga ccaccaccat
       61 cccgacatgc ggaagcgcct cactccagtt gctgaggatc agcggccgca ccgcatcgat
      121 ggcccaggtc atcggattga acctcgccaa aaaactcatc cactccggca ttgccgccaa
      181 cggcaccagt gctgaactca agaatatcat cgggagggag agaaagccca gcacggagaa
      241 gaaatcgcca tggcttttca cggagaaggc catcgccatg gagatcgccg tgagccctac
      301 cccgaacatc atgccgatga gcagaatcgt cgcaatgccg agcagcccgg tggcaggctg
      361 gacgccgaac agaaaggcca cgccgagaat caccaacact tggagggagg taatcgtcat
      421 cacgaagatg aaccggctca ggatcaccga gctccgatgg atcggcgtcg acattaaccg
      481 ttccaagaac ccgttttctt tatcgaagag cagatcgacc cccccggcga gtccattatt
      541 cagcaccgtc atcacgacca cgccagccgc caggaagctg atatagttcg gcgcctgcgt
      601 cacctgcgca tccgccgcac gctggaacag attgccgaag aagatcagcc agaacagcat
      661 cggctggaca agcgtgaaca gcatgctgaa cttctcgcgg ctcaaccgca gcacccatcg
      721 catcgtcaac gcactgattt cctgccagta ctgcgccaca agactccttt atgccgtttc
      781 gctcggcgga accgactcgg taatcgcacg gccggtatgg gcgataaaca catcgtccag
      841 ccgcggccgg ttgtattgaa tgaattccac gcgacagttc agacgattgg cggtgtccag
      901 tattaccggc aaggctttct ccggcgactc gacacggata tcgagtccct tctctgtcgc
      961 ccgcaccgcc ttgatggccg gttgcccctt caacgccgat tctaatgccg gaatcctatc
     1021 ggtttccttc aacgtcaagg agacaatatc gccgccgagc ccgaccttca gttcaaccgg
     1081 cgacccaatc gtcttgatcg ttcctccgtc gatgatagcc agacgatcgc acagctggtc
     1141 cgcttcatcc aagtaattgg tcgtcatgac gaccgtcatc ccccgtgcct tcagcattcg
     1201 gacatagtcc cagatccgga gccggctctg aacatccaga ccgagtgtcg gttcgtccaa
     1261 aaacagaatc ttggggtcgg gcaagaggcc gcaggcgatg tcgagcttgc gcttcatacc
     1321 gcccgagtac gtttttgccg gacgatcagc gtgggcttcg agttcgacga gcttcagcag
     1381 ctcgcggatc cgcttgttgg cctcgtcttt cgggagatgg tacagagcgg ccaggagttc
     1441 aagatgctct ttgccggtca gaaaccggtc gatcgctcgt tcttgcggca catagccgat
     1501 caggtgacgc accttgtcgg cgtcccgcac cgtattgtgt cctaagacgg tggcagtccc
     1561 cgaggacggc gtcagcagcg tgatgagcgt gcggagcgtg gtgcttttcc ccgcgccgtt
     1621 cggaccgagc agaccgaaaa tttccccggt gtagacttga aatgaaatat ctgcgacggc
     1681 gacatggccg tcgtaggact tcgaaaggcg ggagacatcg atcgcgatcg cccggtccat
     1741 cacccccagc ccttcgcgcc gtgatgatcg tgcatgagaa attggatgag atcgctgagg
     1801 cggcgagcct gttggtggaa actatcggct tccaacagaa attgcttttc cagcggtgtg
     1861 cagtccagat aggtcgaaag cgtgttgact aaaatatcat cgctgacatc ttcgcggaac
     1921 caactctgcc aggtcggcgg ttcctggcgc gaacggagat actcttccaa tgcggtcacg
     1981 agtcgctggc gcaccgccgg atccaaacgg gcttccgcct ctctcgtctt gagcgtgatc
     2041 gacgcacggc gatacggttt atcatatgac tcttccgaaa tctcgaatcg ttccatccct
     2101 tgcaagagaa tattcgacct cccgtccgcc agaggctgca cgctgacgag ccggcccacg
     2161 catcccatgg taaacacagg aggatttccg tagtagctcg attcccagcc ttctttcagg
     2221 agagccattc cgatacactg gccgcccatc accgcatcgt cgaccatctg tcgatagcgc
     2281 ggctcgaaga tatggagggg aagatatgtc ttcgggaaca atacgacatt gggaagggcg
     2341 aacaccggaa tcgttgccgg aatagaaaac ggcgtagtct tctcgtgccc atcttgttcg
     2401 tgatgctctc gctcgcgatc agtctgcatg gctcacgata gccgatggtc gacaaaattg
     2461 tcaacggcgt aagaagccgg aatgatgctc cgcgagatgc ggaaaacccg caccaacaca
     2521 gtcgatttgt gcctcgacgg ccggcatgct ataagcgcga acggaagagg tgtcgcgcac
     2581 gctgcggcga caacagatca aatgttccca accggagatg acatgcgacg ctcggctctt
     2641 ttgatatgca tgctgttgac ggtcggaacc gcgctcgccg ccgacatccc gcacgagttc
     2701 acacctgcga cggaagccta ccgctacgaa ttcccgcgtg atcacggatc gcacgaaacc
     2761 tttcgcaccg agtggtggta ctacaccggc aacctcacct caagagacgg ccgcacgttc
     2821 ggctatcaac tcacattttt ccgccgggcc atgccacccg atcaagtcag gacatggccc
     2881 tcaaaatggt ccgtcagcca gctctacctc gctcacttcg ccgtgagcga cctcgacaaa
     2941 aaacaattcc attacacaga aaaactcagc cgggccggtc ttggaaaagc gggggccgag
     3001 gcgaatcact tacaggtctg gatcgatgat tggcgcgccg aatcgccttc ggcacaacaa
     3061 aatctccata tcttgaaggc tgcggagggg ggcctggcca ttcagctcga actctcgccg
     3121 gagaaagcgc tcgtcattca tggaaccgcc gggatcagcc gaaagggagc cgagccaggc
     3181 caagcttctc actactattc gttcacgagg atggcgacgg caggccgcct cacgatcggg
     3241 agcacatcgt ttgaggtgac gggcacaagc tggatggacc atgaattcgg ctcagccgat
     3301 ctcgggaagg atctggtggg atgggattgg ttcagcatac agctcgataa tcggacggaa
     3361 tttatgctct accgattacg ccgtgcggat ggatcggccg atcccgcctc aagcggtacc
     3421 ctcatcgacc gggatgggca ggcgtaccat ctctcgatcg gagattttac gctggaaccg
     3481 atgagttatt ggaccagccc ggcaaacaag gctcggtatc cccaacattg gcgcctgacc
     3541 attccttcga aacaagtctc gcttgacctc atcccgcgcc ttgcagagca ggaactctcg
     3601 acgacacgca gcacacaagt cacctactgg gaaggcgcga tcgagacgac cggcacagcc
     3661 caaggccagc cgatccatgg ccagggttac atggaactga ccggttatgc cgagcgcttt
     3721 gccaagaagt tgtaacggcc gtgctcttct tgactcctcg tcggatcctg tgtttaggtc
     3781 acgcgccatt acaccatgct gattcgaact atcgctcaat tcgcgtccgt cgcgttggtg
     3841 ctcttctgtg ttggttgcca gaaagacagt gaagcgattg tctcgatcgc gctgcatccg
     3901 acgaacgcga acattcttta cgtcgcaacg aacgacgctg tttataagtc acgcgacggc
     3961 ggccagttct gggagaaatt ccccagtttc agtgccagac gcgtcacgac agtggcgatc
     4021 gatccgcagc tgcctgccac gatttacgcc ggcacgatgg gagatgcggt ctataagagc
     4081 cctgacggcg gccaacattg gctgccgcat aatgtcgggt tgaaagaaca cgtctctttt
     4141 gtgaatcagt tcgtcttcca cccggcactg agcgagaaga tctacgcagc cacgacggtc
     4201 ggcgcttact acacgaaaaa tgcgggccgt gaatgggaag agcgcatgaa cggcatgaag
     4261 gaagtccaca tcgtgacctc gatcgcgatc aattccaagg acccgacgat tctttacgga
     4321 ggcaccaccg gagggatcta tcgctcggac gacgccgcga tgtcgtggaa gaaaatcaac
     4381 aacggactga ttccggacac agaactcatg gcgtcgatgg cgttgggcgt gaacgccatt
     4441 gagattgatc gggtcaactc cgaaatcgtg tatgccggga ccacgaaggg actattccgc
     4501 acggcgaaca ggggggaaca atgggagcgg atcgggctgt cgctccccga tccttttgtc
     4561 agcagcatag tcatccaccc cacggagccc tctgcccttt atatc
//
LOCUS       DATIFR010000032        10036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_976287, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000032 DATIFR010000000
VERSION     DATIFR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976287"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="VLD60_01610"
     CDS             <1..1844
                     /locus_tag="VLD60_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSB43676.1"
                     /translation="QEGARYVKDSFHNYVVQGDKEAVNPDHLGTKAAAHYVLTLAPGA
                     MKNIKLRFTNDESVTGFTKQNFDAVFTQRIHEANEFYNSLAPSDLSEDARRVQRQAFA
                     GLLWSKQFYHYDVNRWLKGDPMMPEPPRERWRGRNSDWTHLYNADVISMPDKWEYPWY
                     AAWDLAFHSIPLALVDSTFAKEQLVLMLREWYMHPNGQIPAYEWAFGDVNPPVHAWAA
                     WRVYKIEKKRKGVGDRRFLERVFHKLLLNFTWWVNRKDAEGKNIFQGGFLGLDNIGVF
                     DRSAPLPTGGHIEQSDATSWMGMYCLNMLAIALELARDNRAYEDVASKFFEHFVYICR
                     AMNNIGGEKIELWDREDGFFYDVLHLPDGKTCHIKVRSMVGLIPLFAVETLDSELIDK
                     LPRFKHRMQWFIENRPDFSQHVETESRDGEVRRFLSLVSRDRLRSVLRYMLDEREFLS
                     SYGIRALSRYHKDHPYVFNVMGHEHRVDYEPAESSTGLFGGNSNWRGPIWFPVNYLLI
                     ESLQKFHYFLGDQFTVDYPTGSGNKATLWEVAGEISRRLTHIFLQDGDGKRPVFGTAR
                     VFQEDLHWRDNVLFHEYFHGDSGAGVGASHQTGWTGLVAKLIQQSGE"
     gene            2550..3044
                     /locus_tag="VLD60_01615"
     CDS             2550..3044
                     /locus_tag="VLD60_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43677.1"
                     /translation="MYWIICLFFLLLTGCQGLPRLDQWETAAPPSVMPLWERYQQCLE
                     ANEPATLLQLVEQFEQVMLAGPEPPTWMRSWNLHVRRQPLRTAVDPHALGAACTIRAA
                     KVMAGQDRILEARALYERLISRYTQPEWAYYHGQAQEALASLPILDVALIASVPRPIS
                     PHTR"
     gene            3120..3320
                     /locus_tag="VLD60_01620"
     CDS             3120..3320
                     /locus_tag="VLD60_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43678.1"
                     /translation="MTTRLYWTIQHMNRWMHDRRFAAGCWWLSQQVDFLEWRRRLDEL
                     MPQPILVPIRTQQRSRYAVRSR"
     gene            3342..3605
                     /locus_tag="VLD60_01625"
     CDS             3342..3605
                     /locus_tag="VLD60_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43679.1"
                     /translation="MCCPSCDRMMSSTSRQEIQDENGAMTITRWRCRPCHETAEEIWI
                     RAAYRGQDPIRISYAVATTQVRKSPARSSGGSRRGTLAHAVAC"
     gene            3589..4416
                     /locus_tag="VLD60_01630"
     CDS             3589..4416
                     /locus_tag="VLD60_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSB43680.1"
                     /translation="MRSPVDTLRAHWPEYLMEAAGLGLFMVSASFVTAALEYPHSPLH
                     ATIADPLLRRVVIGFAMGLTAIGIIYSPWGKQSGAHLNPAVTLTFFRLGKIEGIDASF
                     YVIAQSLGGLTGVLLSAAAIGMAIEHPAVNYVVTTPGESGTAIAFVAETIISFGLMLT
                     VLLVSNHRALNVFTGVIAGSLVAIYISLEAPLSGMSMNPARTFASAFTAHLWTSFWIY
                     LTAPPLGMLLAAEVYMWRKGLAQVYCAKLHHRNDQRCIFRCNYLGLLQAGKRSRTQD"
     gene            complement(4422..5468)
                     /locus_tag="VLD60_01635"
     CDS             complement(4422..5468)
                     /locus_tag="VLD60_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43681.1"
                     /translation="MAVSCRFRTLPVILALLAAGASFSVFTTHATQPPEDLAASGGVV
                     PQSGNCSAARPDDERCLPGGPQGQTGPSQSVIPPVPPITPPAPPVTPPITPPPFPLTP
                     WHIDPQGSPPLDPYSALFFTGWAKARLDVNGDDQDITYESNRADLLFWRSNNQQMPGN
                     THYLLTPVPEGPDSKSTPLSVTWTAKGRVGDCTVEGKTAILFYMLHTDIVDPTRPAYG
                     HLYVIGPDGGDFHSVIISAFNPDARLKKTCPGNPPTVTEEPFEAGYLLHIVFRKNMYE
                     ERGLLVLSGMQVLDASKPDAILDMLPPGPGRDIASQALSQAQASPSRTSRVYTWEWAL
                     YPYGNYDEGLGQPH"
     gene            5770..6561
                     /locus_tag="VLD60_01640"
     CDS             5770..6561
                     /locus_tag="VLD60_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HSB43682.1"
                     /translation="MPRILVVLIALVISLTWLPADAGQISRPQAEYSADARVEDEQGT
                     TQQKIFVTPTKERKEMLTGSGEGAVMIFRYDTKTMWTLMPSENMYMEYSLAGAQEQGQ
                     KNDPSQWTYEDTAMGEETLNGVKVTKYKTIATSTDGKKYGGFSWRTKEGINVKQDLLY
                     KEGNDKKRMLTELSNLIIGKQDPKLFEIPDGFTKFDMGGMMGGMMGREGMGQRGMPNP
                     RAQTGRPPRTMPEPETQPTPEPEPPAEEKSDMQKAGDMLKGLFGR"
     gene            6578..7414
                     /locus_tag="VLD60_01645"
     CDS             6578..7414
                     /locus_tag="VLD60_01645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSB43683.1"
                     /translation="MTDCLRGRWLFVAVFVTAVTVLPRAVLADHVTDRPAIKASALYM
                     VELKSGRVLLEKNATRRLPPASLTKIMTALVALESAPLQEVVKIHPRAIVHHSSNHFR
                     AGEKFLLRDLLTAMLVASANDACEAIAWHIGGDDKRFVAMMNERAVRLGLQNTHFANP
                     CGFDAPGHYSTAADLAKLTEQALQQPFFSMMVRTLVRDISTVDGARKLSLHSTNELLV
                     DPDVNGVKTGYTSKAGRCLIASMFKDGQRLLLVALNMMDQWEQASRLLRYGQTVMQGT
                     NG"
     gene            complement(7435..8838)
                     /locus_tag="VLD60_01650"
     CDS             complement(7435..8838)
                     /locus_tag="VLD60_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB43684.1"
                     /translation="MKTNLRAFLAPMAFCTAALIVYAGAFHGDFHYDDSLTILENPHL
                     SNWHTFIDHLDHMVRPILYATFLVDGSLYGFDPAGYHLLNLLLHLGAGFLIYCIIASA
                     VADECSVVPFWTSLLFLIHPIATETVTYISGRASGLMAFFYLLAFFLYIKASEELRTA
                     LPRRYYLSGAVIFFLLAIGSKETAVTLPIILLLWDTVICRLKGPALRAAILLRHSPFW
                     VALLLAAAWAWSHPRYAALAEFSFTIRPLWDHLLSELHAAAYALLLLFSPWNQNFDHD
                     LPVFHSPTQWPLPLDLLLLSAVTAGIFFSARRLPLVAFGLSWFFLQLLPASLIPRNDF
                     LSERNLYLPAIGLLLAIVALGSHLIQWLLTIVRRPALVRFGSSVLATGLIAALCFFTY
                     QRNLLYQDRLLLWSDAVRKSPNKARPHNNLGYAYALRDDWDRAIDEFRAAARLDPDFI
                     VAQQNLRDAYLHRVGRQ"
     gene            8883..9281
                     /locus_tag="VLD60_01655"
     CDS             8883..9281
                     /locus_tag="VLD60_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43685.1"
                     /translation="MASLRMLLSAVSLSITMLLWPPASPAGPEKAGSGGAKSCAELMN
                     EIYKRVDLYFIMGEGKAQEVINDSLPAFKAACPTEAKSIKDLENGNYLKKLAAEREKR
                     NKAWRHVKAAGGFTGTAGQDEDLEDLEIQR"
     gene            9345..>10036
                     /locus_tag="VLD60_01660"
     CDS             9345..>10036
                     /locus_tag="VLD60_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB43686.1"
                     /translation="MRLGQARSVIMTGVVIAFGIPSHSPLVWAASPPAAGQQPATAAS
                     APADDREDQIVAKYLRDCSGKGVKDASCDKQRNDFVAILKEDLLTLGSTADRKYIPDI
                     VRLFRKTPEVELRIDAAHALGMIGPQDQDVNMLIPLTNDPVPDVRHAVSNMLYNGKGN
                     AISLLKQRAVPMRNGQTPDKPSDPAKFSMPVAPDSVYLFDSSDATRGRLSYMVRGKND
                     PAQFFKAKAKKGP"
BASE COUNT         2316 a         2799 c         2960 g         1961 t
ORIGIN      
        1 accaggaagg cgcccgctat gtgaaagaca gcttccacaa ttatgtcgtg cagggcgata
       61 aggaagcggt caacccggat catctgggga ccaaggcagc agcccactac gtgctgacgc
      121 tcgcaccggg cgccatgaaa aacatcaagc tgcggttcac caacgacgaa agcgtgacgg
      181 gcttcacgaa gcagaacttc gatgcggttt tcacccaacg gattcatgaa gccaatgagt
      241 tttacaacag tctggcccca agcgatctat ccgaagatgc acggcgcgtg caacgacagg
      301 ccttcgccgg cctgctgtgg agcaaacagt tctatcatta cgacgtcaac cgttggctca
      361 agggcgaccc gatgatgccg gagccgccgc gcgaacgatg gcgtggacga aactctgact
      421 ggacccatct ctacaacgcc gacgtgatct cgatgccgga caaatgggag tatccctggt
      481 acgcggcctg ggacctcgca ttccacagca tccccctcgc gctcgtcgat tcgaccttcg
      541 ccaaggaaca gctcgtactc atgctgcgcg aatggtacat gcacccgaac ggccagatcc
      601 ccgcgtacga atgggccttc ggggacgtga atccgccggt tcatgcctgg gccgcttggc
      661 gcgtctacaa aatcgagaag aaacgcaagg gtgtcgggga tcgccggttt ctcgaacggg
      721 ttttccataa actgctgctg aactttacct ggtgggtcaa ccgcaaggat gctgagggca
      781 agaacatctt ccaaggcgga ttcctcggcc tcgacaacat cggggtgttc gaccgcagcg
      841 ccccgctacc caccggcgga cacatcgagc agtcggatgc caccagctgg atgggcatgt
      901 attgcctgaa catgctggcg atcgcattgg agctcgctcg cgacaatcga gcctacgaag
      961 acgtggccag taaattcttc gagcacttcg tctatatctg ccgcgcaatg aacaacatcg
     1021 gcggcgagaa gatcgagttg tgggatcgcg aagacgggtt cttctatgat gtgctgcact
     1081 tgcctgatgg gaagacctgc catataaagg ttcgctcaat ggtgggcctg attcccctgt
     1141 tcgccgtaga aacgcttgat tctgaactca tcgacaagct ccctcgcttc aaacaccgca
     1201 tgcaatggtt catcgagaac cgaccggatt tttctcaaca tgtcgagaca gagtcacgag
     1261 acggagaggt ccggcggttt ctgtcgctcg tcagtcgcga tcgactgcgc tcagtgctgc
     1321 gctacatgct ggatgagagg gaattcctct cctcctatgg cattcgcgcg ctctcccgat
     1381 atcacaagga ccatccctat gtatttaatg tgatgggcca tgaacatcgc gtggactatg
     1441 agccggcgga gtccagcacg ggcctgttcg gcggaaattc caattggcgc ggcccgattt
     1501 ggtttcccgt caactacctc ctgatcgaat cactccagaa gtttcattac ttcctcggcg
     1561 accaattcac agtggactat ccgaccggat ccggcaacaa agcgacactg tgggaagtgg
     1621 caggagagat ctcacggcgc ctcacacaca tttttctcca agacggtgac gggaagcgcc
     1681 cggtattcgg cacggcacgc gtatttcaag aagatctgca ctggcgggac aacgtgctgt
     1741 tccacgaata tttccatgga gatagcggtg ccggggtcgg cgcaagtcac caaaccgggt
     1801 ggacgggcct ggttgcaaaa ctgattcagc agagcggtga ataaataaaa tgagatgaag
     1861 atcccgcagc aaggcaagaa gttttatcgt cggcctcgaa gcccatctgc acgaagcttg
     1921 tctgggtcag gtgtcggaga tattcggtgc ggaggcaccg cactacccgc ggggctgcga
     1981 tgctcaggcc tggtcggttg cagagcccct gcgggcactg ctagaagaca ttcgcattca
     2041 ggccataggc accagagtcg caacaaaacg ggtggttgaa cgacagcgta aggaaaagac
     2101 tccttcatcg actcaacaga acagaagcaa tagccttggg cgaggggacg aggcgtcact
     2161 gaacccgtaa ccttttctta gccatcgacc ggagccgact agcgttgcac caaataatca
     2221 aaaattggtt ggctcgtatc acgccctctt gtaaggacgt cacgcacttg gtgtcgcaat
     2281 ctctggatcg tcccctcccc tggacgacga ggcttaccct ccaacttcac tactggatct
     2341 gcgaagcctg cgcgcagtac cgccggcaac tcattcaggt acgcaaagta ttgcagcaaa
     2401 tggcgaagtc ctctagcgcc gcgcaacaag gcgattccca gctctccccg tcggcgaaag
     2461 ctcgcctcaa agaggcactc cgctcaaaaa accaataagc ccctgtctcc tgaactgtaa
     2521 gggactgtga ccatatctga cagagatcca tgtactggat catctgcctg ttcttcctgc
     2581 ttctcaccgg gtgccaaggg ttgccgcggc tcgatcaatg ggaaacggcc gctcctcctt
     2641 cagtcatgcc tttgtgggaa cgctatcaac aatgtctcga ggcgaacgag ccggctacgc
     2701 tgttgcaact cgtcgaacaa ttcgagcagg tgatgttagc gggccctgag ccacccacct
     2761 ggatgagatc ctggaacctg cacgtcaggc ggcagccgct ccgtaccgcc gtcgatccgc
     2821 acgcactcgg tgctgcctgc acaatccgag cggcaaaggt catggcaggg caggaccgta
     2881 ttctcgaggc ccgggcgctc tatgaacgtc tgatctcccg ctatacgcag ccggagtggg
     2941 cgtactatca cgggcaggcc caggaagcac ttgcctctct cccgatcctc gacgttgctc
     3001 tcattgcatc agtcccgaga cctatttctc ctcacacccg ctgaatccgt ctcccggcat
     3061 ctgtaagaac atcggcggtc tcacgaccaa acagcaggac tgtgaaggtg aggtgaccga
     3121 tgacaacacg actgtactgg acgatccaac acatgaaccg ctggatgcac gaccgccgct
     3181 tcgccgccgg ctgctggtgg ctctcacagc aagtggattt cctggaatgg cgccgtcggc
     3241 tcgacgaact gatgccgcaa ccgatccttg taccgatacg aacacagcaa cgaagccgct
     3301 acgcggtgcg ctcacgatag gaatccgaga aggagggaac catgtgctgc ccaagctgcg
     3361 atcgcatgat gtcgagcacc agccggcagg agatccagga cgagaacgga gcaatgacga
     3421 ttacgcgctg gcgctgccgt ccgtgccatg agacggcgga ggaaatctgg atcagagcag
     3481 cctatcgcgg gcaggatccg atccgaatca gttacgccgt cgccaccact caagtgcgca
     3541 agtcgccggc gcgctcctcc ggaggcagca ggagaggaac cctcgcccat gcggtcgcct
     3601 gttgatacgc tgcgagccca ctggcccgag tatttgatgg aagcggcggg gctggggctt
     3661 tttatggtct cagcttcatt cgtaactgcg gcgctcgagt atcctcactc gccgcttcac
     3721 gcaacgattg ccgaccccct ccttcgtcgc gtcgtgatcg gcttcgcgat gggcctgacg
     3781 gccatcggga tcatctattc accctggggc aaacagtccg gcgcacattt aaatcctgct
     3841 gtgaccttga cgttttttcg attggggaaa atcgaaggga tcgatgcgtc tttctatgtg
     3901 atcgcgcaat cgctgggagg attgacagga gtactgctca gtgccgccgc gatcggcatg
     3961 gctatcgaac atcctgctgt caactatgtc gtcacgaccc cgggagagtc gggtacggca
     4021 atcgctttcg tcgcggaaac catcatctca ttcgggctca tgctgacggt actcttggtc
     4081 tccaatcatc gtgcgctgaa cgtattcacc ggcgtgatcg ccggctctct cgtcgcaatc
     4141 tatattagtc ttgaagcgcc gctctccggc atgagcatga acccggccag gaccttcgcc
     4201 tctgcattca ctgcccatct ctggacgtca ttttggatct acctgacagc accaccactc
     4261 ggcatgctgc ttgcggcgga ggtctatatg tggcggaaag gcctcgcgca ggtttattgc
     4321 gccaaactcc atcatcggaa cgatcaacgc tgcatctttc gctgtaacta tttgggactt
     4381 ctgcaggccg ggaagcgctc gcgcactcag gattgaatgg ctcaatgtgg ctgccccaag
     4441 ccttcatcat aattgccata cggatatagc gcccactccc aggtgtaaac ccggctggtg
     4501 cgggagggtg aagcctgcgc ttgactgagt gcttgactgg ctatgtcacg cccagggcca
     4561 ggaggaagca tgtcgagaat ggcatccggc ttactcgcgt caagaacttg catgccactt
     4621 aatacaagca agccgcgctc ctcatacatg ttctttctga atacaatatg tagaaggtag
     4681 ccggcctcga acggttcctc agtgacggtt ggtggattgc cgggacaggt tttcttgagt
     4741 ctcgcgtccg gattgaatgc gctgatgatc acactgtgaa agtcgccccc gtcgggcccg
     4801 atcacatata gatgcccata ggccggacgc gtaggatcca cgatgtcggt atggagcatg
     4861 tagaagagga ttgccgtctt cccttcgact gtgcagtcac cgactctccc cttagctgtc
     4921 caggtgactg acaatggcgt gctcttgctg tccgggccct ccggtactgg agttaggagg
     4981 tagtgagtgt tgcccggcat ctgctggtta ttgcttctcc aaaatagcag gtccgcccgg
     5041 ttcgactcat acgtgatgtc ctgatcatcg ccattaacat ccaatcgggc tttggcccag
     5101 ccggtgaaaa acagggcgga gtatggatca aggggcgggc tgccttgagg gtcgatgtgc
     5161 cacggcgtga gaggaaaagg cgggggagtt atcggaggcg tgaccggcgg agcgggtggt
     5221 gtgattggag gtacgggggg aatgaccgac tggcttggtc cggtttggcc ttgcggcccc
     5281 ccaggcaggc accgctcgtc atccgggcgc gcggcagagc agttgcccga ttggggaacc
     5341 acgccaccgg atgccgctag atcctctggt ggctgcgtag cgtgcgtcgt gaatacggag
     5401 aaggaagcgc cagcggcgag gagcgcgagg atgaccggca acgtgcggaa ccgacaagat
     5461 acagccataa tcctccctcc gcccgacacg tcacgaccga acgttcagcc gtgagccgcc
     5521 ggattgtaat tatggttagc ggaaaagtcc aggcaaccgc cagacaccca acccttcgcg
     5581 cctgttaccc gaatttccgc caactggctg cgaattcttc tcaagaaata ccatctttac
     5641 ttctttctcc gcacgggata cccttcgccc tcagttttcc gtgtccccgt ggcctttgta
     5701 agtcgccacg gaaccaggcc ggccgaacac atctgcctca ccgttcacgc atatcggaag
     5761 gagttcgcca tgccacgcat cctcgtcgtt ctgatcgccc tcgtgatttc gctgacctgg
     5821 ttgcctgcgg atgcaggtca gataagtcgt cctcaagcgg agtattcagc cgatgcgagg
     5881 gttgaagatg aacaaggcac aacgcagcag aagatctttg tgaccccgac caaggaacgg
     5941 aaggagatgt taaccggctc cggtgagggg gcagtcatga ttttccgtta tgacactaaa
     6001 accatgtgga cgctcatgcc gtcggaaaac atgtatatgg agtattcgct ggcaggagca
     6061 caggagcaag ggcagaagaa tgatccgtcg caatggacgt acgaagacac cgcgatgggc
     6121 gaggagacgc tgaacggcgt caaggtgacg aagtacaaga ccatcgcgac gagcacggac
     6181 gggaaaaaat acggcggttt ttcctggcgg accaaagagg gcatcaacgt caagcaggac
     6241 ctgctctata aggaagggaa tgacaagaag cggatgctga ccgagctgag caatctcata
     6301 atcggcaagc aggatccgaa attgttcgag attcctgacg gattcacgaa attcgacatg
     6361 ggcggcatga tgggagggat gatggggcgc gagggcatgg gtcaacgcgg catgccgaat
     6421 cccagagctc agacgggaag gccgcccaga accatgccgg aaccggaaac ccagccgacc
     6481 cccgagcctg aaccgcctgc cgaggaaaag tccgacatgc agaaggccgg cgatatgttg
     6541 aagggcttgt tcgggcgata ggcgatagac aatcgacatg accgattgtc tgcgaggcag
     6601 atggctcttt gtcgccgtct tcgtaacggc ggtcaccgtt ctgccgcgcg ctgttcttgc
     6661 tgatcatgtc acggatcggc cggccatcaa ggcgtccgcc ttgtacatgg tcgagctcaa
     6721 atcgggtcgg gtcctactgg aaaagaatgc cacccgccgc cttccgcccg ccagcctcac
     6781 caaaatcatg accgcgctgg tggcgcttga atccgcgccg ctgcaagaag tggtaaagat
     6841 ccacccccgc gcgatcgtcc atcattcgtc caaccacttc agagccgggg agaagtttct
     6901 cctgcgcgac ttgctgacgg ccatgctggt cgccagcgcg aatgacgctt gcgaggcgat
     6961 cgcctggcac atcggcggcg acgacaaacg gttcgtcgcg atgatgaacg agcgggccgt
     7021 tcgtctggga ttacagaata cccatttcgc caacccctgc ggtttcgatg cgcccggcca
     7081 ttattcgacc gcggcggatc tcgcaaaatt aactgagcag gctctgcagc aaccgttttt
     7141 ctccatgatg gtgagaacgc tggtccgcga catctcgacg gtggacggcg ccaggaaact
     7201 gtcgctgcat agtacgaatg aactcctggt tgatccggac gtcaacggcg taaagaccgg
     7261 ctacacgagc aaggcaggcc gctgcctcat cgccagcatg ttcaaggacg ggcaacgctt
     7321 gttgcttgtc gccttgaaca tgatggatca atgggaacag gcctcacgtc tgttgcgcta
     7381 tgggcagacg gtcatgcaag gaacaaacgg ttgatcggta aaccggagag atctctactg
     7441 ccgtccgacg cgatgaagat acgcgtcgcg gagattctgc tgggcgacga tgaaatcggg
     7501 atcgagccgg gctgccgcgc gaaattcgtc gattgcgcgg tcccagtcgt cccgaagagc
     7561 ataggcgtag ccgagattat tgtggggcct cgctttgttc ggggattttc ggaccgcgtc
     7621 ggaccagagc aggaggcggt cttggtagag caggttccgc tggtaggtga agaagcaaag
     7681 tgcggcgatc aagcctgtcg caagcacgga cgagccgaag cgcacgaggg ccggtcgccg
     7741 gacgatcgtc agaagccatt gaatgagatg cgaaccaagc gcaacgatcg caagcagcag
     7801 cccgatcgcg ggcaggtaga gattgcgctc gctcaagaag tcgttgcgtg ggatcagact
     7861 ggcgggcagc aactgtaaga agaaccagct caaaccgaat gcgaccagcg gaagccgcct
     7921 ggcggagaaa aagatgccgg ccgttacggc cgagagcagc aagagatcga gcggaagcgg
     7981 ccattgtgtc ggcgagtgaa ataccgggag gtcgtgatcg aagttctggt tccagggaga
     8041 aaacaatagc agcagcgcgt aggccgcggc gtgcaattcg ctcagcaggt ggtcccagag
     8101 cggtcggatc gtgaaactga attccgcgag cgccgcatag cgcggatggc tccaggccca
     8161 ggcggccgcg agtagcaggg cgacccaaaa gggcgaatgg cgtaagagta tggcagcgcg
     8221 gagcgccgga cctttcaacc ggcatatgac agtatcccaa aggagcagaa tgatcggaag
     8281 cgtgaccgcc gtttccttgg agccaatcgc cagtaggaaa aaaatgacgg cgcctgacag
     8341 gtagtaccgg cgaggcagtg cggttctgag ttcctcggac gccttgatgt agaggaagaa
     8401 cgccaggaga taaaagaacg ccattaagcc ggaggcgcgt ccggaaatat acgtcaccgt
     8461 ttcggtagca attggatgga tcaggaagag cagcgaggtc caaaacggaa caacggagca
     8521 ttcatctgct accgcgctgg cgatgatgca ataaatcaga aaccctgcgc ccaggtgaag
     8581 gaggagattc aggagatgat agcccgcggg atcgaaccca tacaacgagc catccaccag
     8641 gaaggtcgcg tacaagatgg gacgcaccat gtgatcgaga tggtcgatga aggtgtgcca
     8701 gtttgaaaga tgcggatttt cgagaatcgt gagtgagtcg tcgtaatgaa aatccccgtg
     8761 gaaggctccg gcatagacaa tcagcgcggc agtgcagaac gccatgggcg ccagaaatgc
     8821 gcgaagattt gtcttcacga caccgttata ctgcatcacg ttaactgagt ataggaggga
     8881 ctatggcaag tttgcggatg cttctctctg cggtctcact gtccatcacc atgctcttat
     8941 ggcctccagc gagtccagca gggccggaga aggctggctc gggcggcgca aaatcgtgcg
     9001 cggaactcat gaatgagatc tacaagcggg tcgacctgta tttcataatg ggcgaaggaa
     9061 aggctcagga agtcatcaac gattccctgc cggcattcaa agcggcctgc ccgacggaag
     9121 cgaaaagcat caaagatctc gagaatggga attatctcaa gaagctggcg gcggaacgtg
     9181 aaaaacgaaa caaggcatgg cgccacgtta aggccgctgg aggattcacc ggcacggccg
     9241 gtcaagatga agacttggaa gatttggaaa tccagcggta gatgatttga ggcgagatag
     9301 cacagctctt gtagctggac gagtagattg taaggaggat acgtatgagg ctaggtcaag
     9361 cccgttccgt catcatgaca ggcgtagtca tagcattcgg gatcccttcg cactctcctc
     9421 tggtttgggc ggcatcgcca ccggctgcgg gccagcaacc ggcgacggcc gcttcggcac
     9481 cagccgatga ccgagaagat caaatagtgg cgaagtatct ccgcgattgc agtggcaagg
     9541 gagtgaaaga tgcgagttgc gacaaacaga gaaacgattt cgtcgcgatc ttgaaggagg
     9601 acttgttgac gttaggttct accgccgatc gcaagtacat ccctgatatt gttcgattgt
     9661 ttcgaaaaac acctgaagtc gaattgcgga ttgacgcggc gcacgcgctc ggcatgatcg
     9721 gaccgcagga ccaggatgtg aacatgctga tcccactgac caacgatccg gttccggatg
     9781 tcaggcatgc ggtgtccaat atgctttaca acgggaaggg caacgccatc agtttgctga
     9841 aacagcgggc tgtgccgatg cgtaatggac aaacaccgga caagccgtcc gatcccgcaa
     9901 aattctctat gccggtcgcc ccggacagcg tctatctctt tgactcgagc gatgccacga
     9961 ggggccggct gtcctatatg gtcagaggga agaacgatcc tgctcaattt ttcaaggcca
    10021 aggcgaaaaa aggacc
//
LOCUS       DATIFR010000033        36313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2701239, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000033 DATIFR010000000
VERSION     DATIFR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36313
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2701239"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..359)
                     /locus_tag="VLD60_01665"
     CDS             complement(147..359)
                     /locus_tag="VLD60_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43687.1"
                     /translation="MKRMLMAIMAVAVAVSFSAPSFAAEEKKDKKDEKKGGHVLVYAD
                     EKKDKKDEKKGGHADEKKDKKDEKGK"
     gene            582..1127
                     /locus_tag="VLD60_01670"
     CDS             582..1127
                     /locus_tag="VLD60_01670"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HSB43688.1"
                     /translation="MATMKQPNPPLRDYLNPGLRVLFIGINPGLRSAQVGHHFAGHSN
                     RFWRLLFESGLVNIPLTYQDDWRLPEWRLGLTNIISRPSAGIDDLDPAEYRAGVSALE
                     RKIIRYQPLTVALLGITIFRRLFPEETGRGSSLDLGPTTSRLADTPVFLLPNPSGRNA
                     HYSYEQMLRAFRTLRKEAGSQ"
     gene            1192..1971
                     /locus_tag="VLD60_01675"
     CDS             1192..1971
                     /locus_tag="VLD60_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HSB43689.1"
                     /translation="MLLEGKKGLIIGVANKHSIAWAIAQSAAGQGAQLFFNYQNDRLK
                     ENVEELVATMPGSKAFPCDAGDDAQIDALMTSVGKEAGKLDFLVHSIAFAPREELTGQ
                     FVNTTRKGFATALDVSAYSLVAVTRAAMPLMTDGGSIVTLTYLGAERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPKNIRVNAISAGPIKTLAARGVSGISKMVDHHKEFAPLRRAT
                     EQGEVGDTALFLVSSLGRGITGEVIYVDGGYNILGSLASVD"
     gene            complement(2041..2454)
                     /gene="arfB"
                     /locus_tag="VLD60_01680"
     CDS             complement(2041..2454)
                     /gene="arfB"
                     /locus_tag="VLD60_01680"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HSB43690.1"
                     /translation="MLHISSHVIIPDSEIVIHAMRSQGAGGQHVNKVSSAVHLRFDIG
                     ASSLPPFYKEELMKLRDHRISEDGVITIKAQQHRSQEQNRADALERLRVLIQSVAIPR
                     KKRKATKPTKSSQNRRIESKKKQGRLKALRATLDD"
     gene            complement(2764..2988)
                     /locus_tag="VLD60_01685"
     CDS             complement(2764..2988)
                     /locus_tag="VLD60_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43691.1"
                     /translation="MSQILNLSQHTTDDELQRLTTLLLFHLVEQCGGQVQFKLEDAQR
                     VRSSLTTKMVQMQVGDEVRLRIIDRLPELQ"
     gene            complement(3213..6101)
                     /gene="gcvP"
                     /locus_tag="VLD60_01690"
     CDS             complement(3213..6101)
                     /gene="gcvP"
                     /locus_tag="VLD60_01690"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HSB43692.1"
                     /translation="MGTPEFLNPTDTFVHRHLGPTEADMREMLALLGLQSLEALSDAT
                     VPRDIQLKGALDLPPHRGEQAVLQELHNLAEENRICRSFIGMGYYDCITPGVIQRNIL
                     ENPGWYTQYTPYQAEISQGRLEALVNFQTMITDLTGLLLANASLLDEATAAAEAMTMC
                     LGIARSAGQERKEFFISLDCHPQTIAVMRTRAEPLGMTLHVGRTKTIDFTNPKLSGIL
                     LQYPTTDGYVGDYSAIVEQAHKAGILVVVATDLLALTLLRPPGEFGADIAVGSSQRFG
                     VPMGFGGPHAAFLSAKEEYKRQMPGRIVGVSKDRTGRTAYRLSLQTREQHIRREKATS
                     NICTAQVLLAVMASMYAVYHGPEGLRKIAERVHGFAAILAMGLRQLGFEVGSEEFFDT
                     LRVRVTKAQSEQILMRADELGINLRRYEDESLGIALDEGTGEGDVQRLMEVFVGHERL
                     PITVQELSRTVTTSFPSNLSRTSAYLTHEVFNRYHAEHEMMRYLHRLQARDLSLTHSM
                     IPLGSCTMKLNATTEMLPVSWPEFARLHPFAPLEQSKGYQELFRQLEAWLAEITGFSA
                     ISLQPNAGSQGEYTGLMVIRAYHRSQGEFHRDVCLIPVSAHGTNPASAAMVGMTVVVV
                     ACDQRGNVDLADLESKAAQYRDRLAALMLTYPSTHGVFEADVRRICQIVHTHGGQVYM
                     DGANMNAQVGLCRPGDIGADVCHLNLHKTFCIPHGGGGPGMGPIGVARHLAPFLPGHP
                     VTGLGGAESIGPVSAAPYGSPSIMTISWVYIALMGRDGLTKATQVAILNANYMAKRLE
                     KYYPILYTGTSGFVAHEFILDLRQFKGTAGVEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESESKSELDRFCDAMIRIRAEIQEIADGRQPRTNNLLKNAPHTAQVVSASEWD
                     RSYSREQAAYPAPWVKDNKFWPHVSRIDEAYGDRHLVCTCPPMENYSS"
     gene            6315..6986
                     /locus_tag="VLD60_01695"
     CDS             6315..6986
                     /locus_tag="VLD60_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB43693.1"
                     /translation="MRILVIEDETKVGCFIKRALEEESYAVDLCEDGAKGLEMALATN
                     YDTIVVDLMLPSMSGLDIVKGIRRERIQVPILILTAQSQVDQRVKGLDAGADDYLTKP
                     FAIDELLARIRALLRRGATDSPGVLQIDDLVLNPATREVTRGGQRIDLTLKEYALLEY
                     LMRHSGRVLTRPMISEHVWNQDFDTFTNVIDVYVNYLRNKIDRGRTKKLIHTIRGSGY
                     MLKAD"
     gene            7236..8648
                     /locus_tag="VLD60_01700"
     CDS             7236..8648
                     /locus_tag="VLD60_01700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HSB43694.1"
                     /translation="MPLRLRLTLWYGSALALILIAFSTVLYFVTARSLRDALDQSLEE
                     TAAAAVRSLEERGFLPLIDEDELMSQFPELARIDKFFQIFSPSGTITIRSPNVKQHEL
                     PLSRRALEVAFSGQTIFESAKYPKEPPLRLISVPIVYRGNLLYIVQVGTSMESVDQTL
                     NRLLLVLLVTMPLALAVSLAGGWFLAGRALRPVDAITLAAQRIAAGDLTQRLNVAASP
                     DEIGRLAATFNNMIARLETSFRQIRQFSSDASHELRTPLTVMKGETELALRRPREAGD
                     YTVVLESNLEEIDRMTRIVDELLFLSRADMGEVKMEHLPVKLEALLEDIHRQASLLDQ
                     DREIQVVLGLVTPATVLGDELRLRELFLNLVDNAVKYSRPGGSVNITLATEAGQAKIS
                     VADHGIGISPEDQTKIFDRFYRTDDARAHTKKGTGLGLAICAWIAESHHGRIDVQSEV
                     GKGSIFTVTLPLAPTLLNNF"
     gene            8750..10261
                     /locus_tag="VLD60_01705"
     CDS             8750..10261
                     /locus_tag="VLD60_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSB43695.1"
                     /translation="MSQFVRRTVGTAIAVAALGTVIFWGSQSLTSSHASNSPAPATNS
                     ATLPMAIPAAQGFTDVAKAVTPAVVNITTVTVESVSDGRDGQDELRERMEEFFRDPNG
                     PFGPRGFRGPQGPPEPRGHRGGGQGSGVIVSPDGYILTNNHVIDHAREVTVTLPDKRE
                     FKGKIVGTDPKTDLAVVKIEGQNLPTVAWGDASKLQVGEYVLAVGNPFGLNSTVTLGI
                     VSALGRGRMGITQYEDFIQTDAAINPGNSGGALVNTKGELVGINTAIFSQTGGYQGVG
                     FAVPTSMSKPIYESLIKNGKVVRGYLGIGIQDLNHDLAKSFGLKDAKGALVSDVREDS
                     PADQAGLKQGDVLMSYQGTPVEDAVALQRMVTRTVVGTKVPVKVIREGHEKELTVTIG
                     EQPDSAKIAKAETGEKDFAFAGVAVQDLDRDRAKELGITGKTQGVVVTSVEPDSGAEK
                     AGLMPGDVIREINRQPVKSVKDFEKVSSAVKKGENVLILINRRGASLFLSAKV"
     gene            complement(10303..11169)
                     /gene="purU"
                     /locus_tag="VLD60_01710"
     CDS             complement(10303..11169)
                     /gene="purU"
                     /locus_tag="VLD60_01710"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HSB43696.1"
                     /translation="MTAQKKDAVVLLIHCKDRKGIVARVSGFIHDFGGNILDSDHHTD
                     EETNDFLMRMEFSTEGLQIPPDDIPAAFAPIAKVFDMRYEVHPTSRRTRVAILVSKQD
                     HCLADLLQRHKRDELHIDIPVILSNHDTCASWAELFKIPFAVYPVTKDSKPQQEQQLL
                     SALHAHRAELVVMARYMQILSAEFLAQAGCPVINIHHSFLPAFIGANPYRQAYDRGVK
                     IIGATAHYATQDLDEGPIIEQDVIRIGHRDTVEDLVRKGRDLEEIVLARAVRFHIERR
                     VLVYGRKTVVFD"
     gene            complement(11242..12633)
                     /gene="rimO"
                     /locus_tag="VLD60_01715"
     CDS             complement(11242..12633)
                     /gene="rimO"
                     /locus_tag="VLD60_01715"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSB43697.1"
                     /translation="MIKLGRPTRAKSRPLSGVKTTIGFVNLGCSKNQVDSEVMLGTLV
                     ADGFDLTADPQKAEVVIINTCGFIEEAKQESINTILEHGKLKQHGACRVLIAAGCLAQ
                     RYQGDLLKELPELDGVVGTGEFGKIADICRDLLAPKKRHQRLWISKPPYLYDEMAPRL
                     RLGKQHSAYVKIAEGCNRSCAFCAIPLMRGKQRSRPVESIVAEAQRLASEGVKEINLI
                     SQDTVNYGVDLGMRHGLVTLLRGLVKVKELRWIRPFYLYPQQVTDELLDLYADEEKIT
                     KYIDMPLQHINDRMLKRMHRLGDRATIEKLVERIRGRIPGVTFRTAFIVGFPGETEEA
                     FEELKEYVEKTEFDRVATFLYSDEEQTPAVDFEGKVDRSLMEERRNELLAVQEAIAAA
                     KSREKIGSVLDVLVDGPSEETELLLEGRHEGLAPEIDGVVYINEGTASAGEFVKVEIT
                     EAATYDLVGHIVL"
     gene            12669..13268
                     /locus_tag="VLD60_01720"
     CDS             12669..13268
                     /locus_tag="VLD60_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSB43698.1"
                     /translation="MKSLSISPLRRTGRFPYCAAMIRTFQGIKPTIPPSCFIEETGVV
                     IGDVVMGESCSVWFHAVIRGDVNYIRIGDRTNVQDLCMLHVTHDTHPLIIGNEVTIGH
                     NVVLHGCTIKDRVLVGMGAIIMDGAIIGEDSVVGAGALITEGTVVPPKSLILGSPAKV
                     KRPVTENELAWIKESAENYVKYARQYMGDSSKAKTGFHL"
     gene            complement(13282..14010)
                     /locus_tag="VLD60_01725"
     CDS             complement(13282..14010)
                     /locus_tag="VLD60_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43699.1"
                     /translation="MLQTINKPRRISQSARAAVASLFGRLEYRTLGGIYCYEGGNQFW
                     RAKREPCRRLGMRVAEALLRRLRPGGRSLYVGAGVGELPALVVETMDLQRQVFPYNLR
                     KAEVSSLNRTCRTTSVRFVGKDAAIAKGTFDHLWIVSVLNDPERFPHLAPLSYGRADP
                     VTFNPARFQKERRMVQSIVDRCMPKLSLPGLVTTSTEEVVWIADWCHRKRIPYIVERQ
                     QFPTALVGDPICFIKIGKGARRQA"
     gene            complement(13995..14870)
                     /locus_tag="VLD60_01730"
     CDS             complement(13995..14870)
                     /locus_tag="VLD60_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB43700.1"
                     /translation="MSNRRTRVTRHLHALTGERHPEGNPKALADCARYLAQQFADCGW
                     TTTRQAFRAWGKTYRNILAVKNPSGSRKDHPAPILIGAHYDTVFNSPGADDNASALAV
                     LLEVAYHLKRAAMKGPVWLAAFSLEEHGLLGSRAFVSRLKRTHQQLAGAIILECVGFA
                     SHDEGSQRIPTGVPIAVPSVGNFLAIVGNEESQSLVTTIERNAARYTPALNTVGLAVP
                     GLGMTLPDARRSDHAAFWDEGFRAVMLTDTANFRNPHYHQPTDTIDTLDLTFLEGVVG
                     TVVASVEELAGCYRP"
     gene            complement(14893..15789)
                     /gene="argB"
                     /locus_tag="VLD60_01735"
     CDS             complement(14893..15789)
                     /gene="argB"
                     /locus_tag="VLD60_01735"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSB43701.1"
                     /translation="MNKLIKKANVLIEALPYIRTFRGKTIVIKYGGNAMTESSLKERF
                     AQDIVLLKYVGINPVIIHGGGPQIDKMLDRLGIESKFLHGVRVTNEATMEIVEMVLAG
                     KINMEITDLINRHGGSAVGLSGKDGGLILSKPLTAKAWAESLERDLDEEDADSDFGFV
                     GDIEKVDPSLLFKLQGDHYIPVIAPIGTDREGNTYNINADLVAGAIAGALRAEKLVMM
                     TDIKGIRDAKGRHLSTVSRKDVQRMVKKGTITEGMLPKVHACLDALEQGVGKAHIIDG
                     RMPHAILLEVFTRKGIGTEITA"
     gene            complement(15888..16826)
                     /locus_tag="VLD60_01740"
     CDS             complement(15888..16826)
                     /locus_tag="VLD60_01740"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HSB43702.1"
                     /translation="MDDAIRAFITFLTVERGASPATLRNYASDLRQLRSFLLSARLAD
                     PPVDPAALSPDSIRAYLQWLDRKGEKRTSLARKLACIRSFYRYLVRTGLVSRSPVEDL
                     RTPKQPKHLPRVLTKDDAEALMAFPDQGTIGSLRDRALLETLYSTGARVSELVSMNVE
                     DLHQPEGVVRLRGKGRKERIVPIGDLAIQAIQTYRGSLQPSTSGNVRLAPVFLNHRGG
                     RLTSRSVARIVARYSSRLTGGSVSPHTLRHSFATHLLDEGADLRAIQEMLGHASLSTT
                     QKYTHVATDQLMAVYDKSHPRAGRTHADRAAKERKR"
     gene            complement(16799..18115)
                     /gene="trmFO"
                     /locus_tag="VLD60_01745"
     CDS             complement(16799..18115)
                     /gene="trmFO"
                     /locus_tag="VLD60_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HSB43703.1"
                     /translation="MRDDIVIIGGGLAGSEAAWQAANRGARVTLYEMRPKEMTQAHNT
                     GGLAELVCSNSLGSVDVLNAPGILKEEMRRLNSLIIKVADEVRVPAGSALAVDRDQFS
                     FKVTQALESHSNIRIRREEVTEIPTDCLCIIATGPLTSDKLSQAIAQLTHSNHLYFYD
                     AISPIVDAESINMDIAFRASRYDKGGDDYLNCPMDEATYNKFYDALLAAEKVQPKDFE
                     KTPYFEACLPIEVMAERGRQTMQFGPLKPVGLEDPRTGVRPYAVVQLRTENAYRSCYN
                     MVGFQTKLTYGEQKRVFRMIPGLEQAEFLRYGSLHRNTFINSPRLLRDTLQFKARGTL
                     FFAGQLVGVEGYTDSAAMGGLAGINAARGLAGLPLVAPPPTTAHGCLLSYITTADPRH
                     FQPMNTNFGLFPPLASPVRDKERKRQLISQRALEDHAAWMTRSGLS"
     gene            18334..18408
                     /locus_tag="VLD60_01750"
     tRNA            18334..18408
                     /locus_tag="VLD60_01750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18366..18368,aa:Val,seq:cac)
     gene            18553..18888
                     /locus_tag="VLD60_01755"
     CDS             18553..18888
                     /locus_tag="VLD60_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43704.1"
                     /translation="MKIRACATGILLLTMVVGCAGNRQQSMDADGNYVAPASIYSVMD
                     STSLVYTDVAAGSPLNDNPWRWLGFLLHPVGQVLDYGFNRPVYSLTGKMPYLFGYTSE
                     DAMLDSTRQ"
     gene            complement(19046..19273)
                     /locus_tag="VLD60_01760"
     CDS             complement(19046..19273)
                     /locus_tag="VLD60_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43705.1"
                     /translation="MGRASISWDYQFLSRPPDLYRGRRMGLKKIGFTLTQASRLEEYQ
                     VSLGRRQILARRGCFASGDETGEAKCTGEYE"
     gene            19310..20170
                     /locus_tag="VLD60_01765"
     CDS             19310..20170
                     /locus_tag="VLD60_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB43706.1"
                     /translation="MVGALFTFSSGLDLAPYSVLRHTLTLLKPSLEFYLNVANFAAKS
                     DMDSSSLYETGDEKTTTQYLVIGGHEHPALLQASITIDPYVRLFSPYRREFLTRLRQP
                     GGQKQGQQVDSQGAEDKEPFPSLQQFARGQTAQLSYCGAQHYEIAADAYHKAITSGFT
                     NKIWVAEVHHKYGQTLFGLGQYEKAKAEMLQSLAIRPNTPEILNNLGSVQQKLGEKAA
                     AQASFEKAITLRPNYAIARFNLAEAVESTNPKRAITEYETYLALVEGIPEEADRIALV
                     QQRLKALKHQ"
     gene            complement(20231..20668)
                     /locus_tag="VLD60_01770"
     CDS             complement(20231..20668)
                     /locus_tag="VLD60_01770"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HSB43707.1"
                     /translation="MKIRTSPKPTARTSGKSLASKGEASTAVISKKYSAIYRDLQRQR
                     AAILEEASEGLTKREGQEAFPDVSDQASAEVDQNFTMRIKEREQKLLKKIDEALDRMK
                     KNTYGICERCEEEIPYQRLKARPVTTLCIACKTLQEHEEKIRR"
     gene            complement(20772..21374)
                     /gene="recR"
                     /locus_tag="VLD60_01775"
     CDS             complement(20772..21374)
                     /gene="recR"
                     /locus_tag="VLD60_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HSB43708.1"
                     /translation="MAVDQQGLLARLVRELVRLPGVGQKTAQRLAFHVLKAEREDALR
                     LAEAIRAVKDGLSFCQQCRNIAEGELCEFCLDPKRDRTKILVVEEPSTLYAIERAGGY
                     RGLYHVLLGALSPLDGVGPSDIRAEELIDRVRLGGIQEVIVATNPTIEGEATAIYLTN
                     QLKPLGARVSRIAYGIPVGMDIEYADEVTLLKSIEGRREL"
     gene            complement(21388..21711)
                     /locus_tag="VLD60_01780"
     CDS             complement(21388..21711)
                     /locus_tag="VLD60_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSB43709.1"
                     /translation="MKNPFGNMSSIMKQAQAMQAQMAKLQEEAATKTVTGSAGGGMVT
                     VTANGAMELVSIVIDPEAVKSGDADMLQDVIMAASNDALRKARQMMADQMKAVTGGLN
                     VPGLF"
     gene            complement(21708..23501)
                     /gene="dnaX"
                     /locus_tag="VLD60_01785"
     CDS             complement(21708..23501)
                     /gene="dnaX"
                     /locus_tag="VLD60_01785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HSB43710.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLVNSVNTKRIAQAYLFS
                     GTRGVGKTTVARILAKALNCEQGPTGTPCATCANCQEIAQGTSVDVIEIDGASNTSVD
                     DVREIRENVKFAPFRGKYRVYIIDEVHMLSNSAFNALLKTLEEPPPHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIARTEIIDRLRHVAQQDGIAIEERSLTALARASEGSMRDGL
                     SLLDQAVAFGGKSVVHEDLEALLGAVPQELLREMIRAITLQDSAAALRVLAQLLDQGH
                     DLRAFCAEVVEYLRHMLVVSVTSQQEWPKLIEASSEDLAQIAADKGSLTPEQLQQIFA
                     LMTQAEDALRLSAHPRFVLETAAVKATRLFLQESEKAGPAVRATPSERPPVPSSSRSD
                     KATSPERSAAPAPSRPTTPVPKPSPSSGPVPRTGGGAMAQTVKSAIAEPTKPMDSAPT
                     TSLRIVQTVALNWEQFQDDVSHAFPNIAPFLEMGRLVAIEGNVVTIGFAKQATVARGM
                     LEKPDNIQTLASLCERISGQPVRIRIVELTEADPPGPTMAQIRAAKEQDQKLVLFERA
                     KAHPVVKQALEIFGADLADVRAVSNQKEVQE"
     gene            complement(23606..23705)
                     /gene="ffs"
                     /locus_tag="VLD60_01790"
     ncRNA           complement(23606..23705)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLD60_01790"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(24002..24091)
                     /locus_tag="VLD60_01795"
     tRNA            complement(24002..24091)
                     /locus_tag="VLD60_01795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24055..24057),aa:Ser,seq:gga)
     gene            24207..26084
                     /locus_tag="VLD60_01800"
     CDS             24207..26084
                     /locus_tag="VLD60_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSB43711.1"
                     /translation="MKIVIGLIVVIVLLVGGILSLPFLVDLNKYQDHYKPLIEEALNR
                     KVQLQDIRLTIWPRIGARVAGFTVLDDPSFSSGPFASLTSLDVGVKLLPLLSGKVEVE
                     EITLREPVLTVITNKNKVMNVSTIGPKTPTPAVESGAPSQSHGDPLQALALLAVDQLS
                     IDGGRLTYRDLSTTPVTEYQIEGLELALKSVRLGQAPTLHFAATVQPYNLPVALDGSF
                     GPLVERLEVKQYDFTLGFGKVALGLKGALLDGTLNATLSSPSISTADLPVTLPLNKPV
                     QIKDLRVVAKAPYPLKQGVSSMELADVTDLDLALVMGNSSLNVKGTVLGGHAKLTLAS
                     PSINTADLPVDTGFQKPVEITHLEANADLKGQEARLTNLSFRLFNGQAKAQGGMSTAT
                     ATPPFNGMVTIQGLQLGPVLDAVSPDSKVSISGTAAMDIAVAGRGFSMPDLTKTLEGP
                     GHLEVKEGKIEGVNLMEEAVTLLKVAGISLDRANATAFSIIETDFTIRQGIINVQKLL
                     MDSHDFQATGNGTVGFDQRLNLAMNLNLSQTLSQKLAGSSPIAKLALRDGRLRLPLLI
                     TGTVQAPSYRLDARGLSGKVQEQVQEKVKEAIGGLLKGESKPSDLEKQGQDLLKGLLD
                     R"
     gene            26406..27512
                     /locus_tag="VLD60_01805"
     CDS             26406..27512
                     /locus_tag="VLD60_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSB43712.1"
                     /translation="MIQTVLVRALLTMTLLIGLVSTVEAQTVPPAPEPAPLDIVTLKD
                     GSILYGEVIGMESGVLHLKTPASPDNVVKVKWESIAKLAVNHPLPFHLKEGSVLIGTA
                     TEGPAGTIMVQAEPLKGTMEVPLDSIAAVNPIVQAPVIYQGTLTGGYSQATGNSHLRN
                     ASLIGDFVARGEQLRLTLNGRYVYGDKDNELSARNARGTIKMDFFITKRFFWFVSAYF
                     EKDTFQDLKLRTALSSGPGYQIIEKGDYNSPWLKDLTLYFDAGLAYFNEDFTTAPDET
                     SMRGRWAVRLNWPMLDDRITVYHYTEFYPSLENTKDYYLTMDNGVRFKLIDGFVSGIQ
                     VTTRYNSNPAQGTGDTDNLYLLTLGYSFDTTRKR"
     gene            27553..27981
                     /gene="mscL"
                     /locus_tag="VLD60_01810"
     CDS             27553..27981
                     /gene="mscL"
                     /locus_tag="VLD60_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSB43713.1"
                     /translation="MLKEFRDFAMRGNVIDMAIGVIIGGAFGKIVSSLVSDVMMPPIG
                     LLMGKVDFSSLFINLSGTPQPSLTAAKAAGAPTINYGVFLQATFDFIIIAFVIFMLVK
                     QVNRLKKEPPAGPPTTKDCPHCLSTIPIKAVKCAHCTSTV"
     gene            28026..29150
                     /locus_tag="VLD60_01815"
     CDS             28026..29150
                     /locus_tag="VLD60_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB43714.1"
                     /translation="MMRWFKTAVLATGLLASASYASAVDLGAICCDGGYEYRHPNRWE
                     LELQRLKDELAAAQKQLADRDREIASLRSGSNEALRSAQADLERSKTRIGDLERQLAD
                     RDREIASLRSGAGDSSKLASQLSSTQGELAQSQARTAELERQLAALQASQAGLQASSG
                     DKDRMAAELAAAQAQLAALQAGAGDKDKLAAECAAAKQRIAELERQLADRDKELAGLR
                     GDLSAEMAKLKEAQRGLVRALRPQIEKGTITVDLNNERLLINLASGYLFGSGEDQLKP
                     AGADALKQVGTILKEFPEYKVAVDGHTDNRPIRGSLKKKFPTNKELSEARAANAAKAL
                     AEGGLTSSTTNGYADTKPVEPNTTEAGRAKNRRVEVRVTR"
     gene            29241..29702
                     /locus_tag="VLD60_01820"
     CDS             29241..29702
                     /locus_tag="VLD60_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43715.1"
                     /translation="MNQMIMILSIAAVSIVYQTNAGAADSTQQLPTLGGIKSAVQQEA
                     NQEKQAAKDAAQAEKSKAEQAVAAEKQKAKDIVESKKSKANAKAAEQQQRAKALAAEK
                     KAKAEAMAAEQKQKATDKAQSQVDQTLQKASEKTDASMGKVDSKLDSLLGK"
     gene            complement(29760..31004)
                     /locus_tag="VLD60_01825"
     CDS             complement(29760..31004)
                     /locus_tag="VLD60_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB43716.1"
                     /translation="MGKMSRRRFLQVSAVTGGALLLGDLLGQVLGRLPAARVAHAAEP
                     IRIGILDPLSSPYKTSSIHDVHGANVAVDLFNKNGGVLGRPVVILEADDESNPETAVK
                     VATKFIKEDRVDVLLGTFNADCALAVSALAQQENKLFMVTGSHLPELTGAACNRHTFV
                     FMPNASMMSRAVAPYLLKAYGNKWYMITTSTLDGKAMAQAMVTAGQSLGVEMVGEALM
                     PFGSTEFSSALTAAKEKRPTLILLNLYGWDLIHALKAYTKLELAKEKIGIGGMIGGEQ
                     IGRPLGYANNAGIWGLIWDPKVNTDSSRRFIQGVIDKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGTTETTALIKAMEGHEFDGLKVGKSSFRAWDHQHVQDVLVGEAFGKELGLGHY
                     KILATVAGESIAGNRDESGCHL"
     gene            complement(31100..31699)
                     /locus_tag="VLD60_01830"
     CDS             complement(31100..31699)
                     /locus_tag="VLD60_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSB43717.1"
                     /translation="MVTKYLDLTFTDAVRRAQKHYYGRAATIKDAPERDPLTRAEAEF
                     IAARDSFYLGTVSESGWPYIQHRGGPPGFLRVINETTVAFADYTGNRQLLSTGNVSVN
                     DRVALFLMDYKHRERLKILGHARIEDARAHQELVTQLADPKMRSRVERLVFIDVVSFD
                     WNCPKYITPRYPLEEIEELVGPLKRRIAELEAALRAANV"
     gene            complement(31772..32146)
                     /locus_tag="VLD60_01835"
     CDS             complement(31772..32146)
                     /locus_tag="VLD60_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSB43718.1"
                     /translation="MKTAIIIMSDPKNGSDEALGRVFNALALASECKQKGEEVAVVFN
                     GTGTRWPAELTKLSHPANGLYNSVRDVVQGASCGCAEVFEATEGIKSCGVPIMKDHTL
                     AGTGGLLSLRRYLAEGWNTVVF"
     gene            32333..32959
                     /locus_tag="VLD60_01840"
     CDS             32333..32959
                     /locus_tag="VLD60_01840"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HSB43719.1"
                     /translation="MTTGHVRTQQPFLFVGNHLCLDFVNTQMIVRGKPTDLLGGYEDL
                     VAWLVQAKMVNAALATAVMTQWNHKDQEQLFEEGILFRKILREMAGRIVARKTIPGSV
                     VTSINRLLSRCPGYPQLVYTKGRFERHFQSLATQKDGLLAPLAEAAGDLLCSGNLSLV
                     KKCGNAACILYFYDTTKNHTRHWCSMQLCGNRMKVAAHYHRKRDNPTH"
     gene            complement(33037..34116)
                     /locus_tag="VLD60_01845"
     CDS             complement(33037..34116)
                     /locus_tag="VLD60_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB43720.1"
                     /translation="MRVTLLRLILAYIIIAIIPLSAWAYRDYFTAEQKAQLDKIQIVL
                     VDVIVLTDKGAGDAGPIAEVVARRLRELGYTTVQDPTKPHDAVFKVKCEQRKIWEGTT
                     AAGGDADLPDAPSRLWKGPACQLTYRLGDMKVKWQKEVRTNFEDAGQAALSANAGDPG
                     AFAASKLKEELEKSDFPLLLAAEWGHPERLLKLLDAADTPQEKKFRIISLLGEMQADE
                     ALPKLKQVLQDRDLSKQAIGAMGNLGKEGIPLLVDIMNTSPQIELQAAAAKGLGQLGG
                     THGDASVVPPLLAKLQDPKTDWVVLTEVAWSLGRIPDKRSIQPLYDLDRKLQAMRDPD
                     NIPLKKLKEAVFWAIKQCDTWDQFS"
     gene            34482..34564
                     /locus_tag="VLD60_01850"
     tRNA            34482..34564
                     /locus_tag="VLD60_01850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34516..34518,aa:Leu,seq:tag)
     gene            34626..35945
                     /gene="tig"
                     /locus_tag="VLD60_01855"
     CDS             34626..35945
                     /gene="tig"
                     /locus_tag="VLD60_01855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSB43721.1"
                     /translation="MKMEVTELGPMKRALKIEVPAEEVAQRFVRAYSELNRQVHIPGF
                     RPGKAPVALLEKRYSKAVEEDVVRSLVPDFYDRAVRQAGISPVLVEIPPLERVKIKKD
                     APFTFTATVEIKPKIELRDYKAPNPISLKPDKRTVTDEQLARGMEVLREQHARLDAPA
                     AGHKVSEGDFVILSLEGRLDGKPLEGSKKEGHLHKVGSKATVLGLEIDSHVLGKTEGE
                     TVEITQPYPSSHPDARVAGKTVIFTIMVNAVKVKKLPDLDDEFAKDCGAYNSLDELKD
                     KLRGELEKALKREIEDTYKDTIVKRLVETHHFDLPETLVERELEAIIRQHMQQRQRRA
                     GGGEGAAAPDTEDLTKLRQDNRDEAGRRVKVGLILEAIAEKENLTVTQEDLNNEIHRL
                     ATELKIAQADLVKMIQAGGQDSIDELRARILAEKSLDFVYRNAVIQG"
     gene            36006..>36313
                     /locus_tag="VLD60_01860"
     CDS             36006..>36313
                     /locus_tag="VLD60_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSB43722.1"
                     /translation="MLVPIVVEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLVI
                     AQLLFLEAEDPEKDINLYVNSPGGSVTAGLGIYDTMQYVKPPINTICLGQAASMGALL
                     "
BASE COUNT         7847 a        10562 c        10049 g         7855 t
ORIGIN      
        1 cgagttggaa cgattcgcga tgcgaaaacg atggttgggt tacagtcggt ctttatcggg
       61 aacgggaacc ggtaggagaa ccgcttaagg agccctccgc ttttggcgga aggcccctca
      121 agccagatag cctcgccaaa aagggattac ttgcccttct cgtctttctt atctttcttc
      181 tcgtccgcgt ggccgccctt cttctcgtcc ttcttgtcct tcttctcgtc agcgtacacg
      241 aggacgtggc cgcccttctt ctcatccttc ttgtccttct tctcctcagc ggcgaaagaa
      301 ggggcgctga aggaaaccgc caccgcaact gccatgattg ccatcaacat gcgcttcata
      361 atatgtcacc tcccttaatg ttgtgagata tgtgccctca gtaggcactg gctggaggtt
      421 gagcaagctt ggtgcccagt gattgcaaat actaaatcat agtaatatca gccagttagc
      481 cgtttttttg tatattagct tgcatgccga ttcatccaat ttgctgtggc agaatagttt
      541 ggccagacta aaccgcctca gcgtcagccg gttggaatca catggctacg atgaaacagc
      601 ccaatcctcc tcttcgggac tatctgaatc cgggattacg ggtcctgttt atcggcataa
      661 atccagggct cagatcagca caagtcggac accattttgc cgggcactca aatcgcttct
      721 ggaggctcct cttcgagtcc ggcttggtca atattcctct gacctatcag gatgattggc
      781 gtctcccaga atggagattg gggctgacca atatcatcag tcgcccaagt gcgggaatcg
      841 atgacctgga tccagctgaa taccgggcgg gtgtttccgc actagagcgg aaaattatcc
      901 gataccaacc gctgaccgtc gccctgcttg gcattacgat ttttcggagg ctctttcccg
      961 aggaaacagg caggggaagc tctctcgatc tcggccccac cacttcacga ctcgccgaca
     1021 ccccggtctt tctcctgccg aaccccagcg gccgcaatgc ccattattcc tacgagcaga
     1081 tgctccgcgc ctttcgaaca cttcgaaagg aagccggttc acagtgaccg cgaatcatgc
     1141 tatcttcaca cgcattgccc atcagatgtt gatcaaggag ccatacagac catgctatta
     1201 gaggggaaaa aaggcctcat catcggcgtc gctaacaagc acagcatcgc ctgggccatt
     1261 gcccaatcag cagccggaca gggagcgcag ctcttcttca actatcaaaa tgaccgtttg
     1321 aaagagaatg tcgaggaact ggtggccacc atgccgggtt ccaaggcttt tccctgcgat
     1381 gccggagacg atgcccaaat cgacgccttg atgaccagcg tcgggaagga agccggcaag
     1441 ctggactttc ttgtgcattc catcgccttt gccccacggg aagaactcac cggccaattc
     1501 gtcaatacca cccgcaaggg ctttgccacc gcgctcgatg tgagcgccta ctcgttggtg
     1561 gccgtcacga gagctgcgat gccgttgatg accgacggcg gatcgatcgt cacattgaca
     1621 tacctgggag cggaacgggt ggtcccccat tacaacgtga tgggcgtcgc caaagctgcg
     1681 ctcgaagcca ctgtccgcta cctggcccac gatctcggcc ccaagaacat ccgcgtcaat
     1741 gctatctccg ccggcccaat caagacgctc gcagcgcgcg gggtttccgg catcagcaag
     1801 atggtggacc atcacaaaga attcgccccc ctccgacgag cgacagaaca aggcgaagtc
     1861 ggcgacacgg cattgttctt agtgagctcg cttggacgtg gaattaccgg agaagtgatt
     1921 tacgtggatg ggggatataa catcttgggg tcgctggcct cagtggacta gcgtggagta
     1981 ccgttcatct ctcgtacctt gtttgacgga attcgcgctt gaggagttcc gattcacgat
     2041 ttagtcgtct agggttgccc tcaacgcctt cagtcgtccc tgtttcttct tgctctcaat
     2101 ccgcctattc tgagaactct tcgtcggctt tgtggccttg cgcttcttcc gtggaatcgc
     2161 cacactctga atgaggactc ggagccgctc cagcgcatcc gcccggtttt gctcctgact
     2221 ccgatgttgc tgagctttga tcgtgatcac accgtcttct gatatacggt gatccctgag
     2281 cttcatgagc tcttctttgt agaacggcgg taacgaagac gcgccgatgt caaaacgcaa
     2341 atggacggcg ctcgacacct tattgacgtg ctgccctccg gctccctgcg accgcatcgc
     2401 atggatgaca atttcagagt cggggatgat gacgtgtgat gagatatgga gcatcgaatt
     2461 tcgttaagac aagtgcccac cttgtctttc cagagcaaat gcacactcgt taatggtcga
     2521 gtctactgta atcgcttatg cgtatccagt tgcatgcttg gccggtgatg gcggtcctgc
     2581 tgaccaacca ccgacgagag ggtatccgtg ataactctgc cacccagagc gcagactaaa
     2641 tatgcccgag gggcatctcc aaacaggttc gcttgacgga cttgaatgtg ttgttctgag
     2701 aggcacgaat cagcagggtg gctgggtgga actgaatgag cgttgagaaa ggactgacga
     2761 aatctattgt agctccggca accgatcgat gatgcgcagt ctcacttcgt cacccacctg
     2821 catctgtacc atcttggtcg tgaggctcga tcggactcgc tgggcgtctt ccagtttgaa
     2881 ttgcacttgt ccgccacatt gctcaacgag gtgaaacagc aacagcgtcg tcaaacgctg
     2941 gagttcatcg tcagtcgtgt gttggctcag attgagaatt tgcgacatga aaaagccccc
     3001 gcttcggtca agaggtgact atcccttatc gccgaagcga gagactttct acaatgcccc
     3061 aaagggaggg ggaggccagt ttactgatgc aggattccga cgggcgctta ttctgataat
     3121 gctgagttat ttcacaacta tcaatggctt acgatgtctg agcacagggc attcctgacc
     3181 tgctcatttt ggtggctgga gcacagtcga acctaactag aatagttttc catcgggggg
     3241 caggtacaca ccaggtggcg atccccgtag gcttcatcga tacggctgac gtgtggccag
     3301 aatttgttgt ctttgaccca gggagccgga taagcagcct gctcacggct gtaggaccga
     3361 tcccactcag aggccgaaac gacctgtgcc gtatggggcg cgttcttgag gagattattg
     3421 gtacgcggtt gacgcccgtc ggcgatctct tgaatctcgg cgcggatcct gatcatcgca
     3481 tcgcaaaacc gatccagctc ggatttcgat tcgctctcgg tcggttcgat catgagagtg
     3541 ccggcaacgg ggaatgacac cgtcggggcg tggaagccat agtccatcaa ccgctttgca
     3601 acgtccattg cttctacgcc tgccgttccc ttgaactgac ggagatcgag gataaactca
     3661 tgggccacga accccgacgt ccccgtatag agaatcggat aatatttctc caaccgtttg
     3721 gccatatagt tggcgttcaa gatcgcgact tgcgtggcct tggtcagtcc gtcacgaccc
     3781 attaacgcaa tatagaccca tgaaattgtc atgatactgg gactgccgta cggcgccgct
     3841 gaaaccggtc cgatcgattc ggctccgcca aggccggtca ccggatggcc tgggagaaat
     3901 ggagccaagt gtcgagcgac gccgattggc cccatcccgg gtccgccccc cccgtggggg
     3961 atgcaaaacg tcttatgcaa gttgaggtgg cagacatcgg ctccgatatc accggggcgg
     4021 cacagcccga cctgcgcatt catgttcgca ccgtccatgt agacctgtcc accatgggta
     4081 tggacgatct ggcaaatgcg ccgcacgtcc gcctcgaata cgccgtgagt tgagggatag
     4141 gtcagcatca atgcggccaa ccgatcgcga tactgtgccg ccttggattc gagatccgcg
     4201 agatcgacat tccctcgttg atcgcacgcg acgacgacca cggtcatacc caccatggcg
     4261 gcgctggccg gattcgtccc atgtgctgaa acaggaatca ggcagacatc ccgatgaaat
     4321 tccccctggc tccgatggta ggccctgatg accatgagcc cggtatactc gccttgagaa
     4381 ccggcattgg gctgtaagga gatagcggag aaaccggtga tctcggccag ccaggcttcc
     4441 aactgacgga acaattcctg gtaccccttg ctctgttcga gcggggcaaa gggatggagg
     4501 cgtgcaaatt ccggccagct caccgggagc atttccgtgg tggcattgag tttcattgtg
     4561 caggacccga gcgggatcat ggaatgggtc aacgacaggt ccctggcctg gagccggtgg
     4621 agataccgca tcatttcatg ctcggcgtga tagcgattga acacctcatg cgtgaggtag
     4681 gcgctggttc tggacaggtt tgaaggaaag ctcgtcgtga cagtacggct tagctcctgg
     4741 actgtgatcg gcagtcgttc gtggccgacg aaaacttcca tcagccgctg gacgtcgcct
     4801 tcccccgttc cttcatcgag cgcgatgccg agcgactcat cttcatatcg acgaagattg
     4861 atgcccagtt catcagcccg catcaaaatc tgttcgctct gcgccttcgt cacacgcaca
     4921 cgcagcgtgt cgaaaaactc ttcgctcccg acctcgaatc cgagctgccg aagccccatc
     4981 gcaagaatcg cggcgaatcc atggacccgc tcggcaatct ttcgcagtcc ctcaggaccg
     5041 tgatacacgg cgtacatgct tgccatcaca gccaacagca cttgggctgt acaaatgttg
     5101 ctcgtcgcct tttcgcgcct gatatgttgc tcgcgggttt gcaacgagag ccggtaggcc
     5161 gttctaccgg tccgatcttt tgatactccg acgattcgac caggcatctg ccgcttgtat
     5221 tcttccttgg ccgaaagaaa cgcggcatgg gggcccccga atcccatcgg cacgccgaac
     5281 cgttggctgg aaccgaccgc gatatcggcc ccgaattccc ccggcggacg taagagggtc
     5341 agcgccagca gatcggtcgc gacaacgacg aggatccccg ctttgtgtgc ctgctcgacg
     5401 atcgcactat agtcacccac atacccgtcc gtggtcggat attgcagcag aatcccgctc
     5461 aacttcgggt tcgtaaaatc aatcgtcttt gtgcgcccca cgtgcagcgt cattcccagc
     5521 ggttccgccc tggtccgcat gacggcaatc gtctgggggt gacaatccaa tgagatgaag
     5581 aactccttgc gctcctggcc ggcactgcgg gcgatgccca gacacatggt catggcttcc
     5641 gccgcagcgg tcgcctcatc caacagtgaa gcattcgcca agagcaggcc ggttaaatcg
     5701 gtgatcatcg tctgaaagtt gaccagtgcc tccaaccgac cctgggagat ttccgcctgg
     5761 tagggcgtgt attgggtgta ccacccggga ttttcgagaa tgttccgctg gatcacgccc
     5821 ggcgtgatgc agtcgtagta acccatcccg atgaaagacc gacaaatccg attctcctcg
     5881 gccagattgt gcagctcttg cagcacagcc tgttcaccgc gatgaggggg aagatccagc
     5941 gcgcccttca gctgaatatc gcgcgggacc gtcgcatcgc tcagggcctc caacgactgg
     6001 agtccgagca gcgcgagcat ttcacgcatg tcggcttcgg tggggccgag atgacgatga
     6061 acaaacgtat ctgtcggatt aagaaattct ggagttccca tatcggctct ttgaccctct
     6121 gtctgcgttg gtctgtattt tgtttcaggc acgccgggcg aacattatca tgcttgcccc
     6181 ttgttctcca agcagagatc gtcgccttcc tatgagtatt ttctgccagg accaatggag
     6241 ggggtgagca gccggctcgt aacatccact ggagatcgac agccctaggt ttgctataat
     6301 cttccatcgc caccatgcgt attctggtca ttgaagacga aacgaaagtg ggttgcttca
     6361 tcaagcgggc gcttgaagaa gagagctatg cggtcgactt gtgtgaagac ggggccaaag
     6421 ggctggaaat ggcgcttgcc acgaactacg acacgattgt tgttgatctc atgcttccgt
     6481 caatgtccgg cttggacatc gtcaaaggga tccggcgcga gcgcatccag gttcccatcc
     6541 tcatcctgac cgcccaatcc caagtggacc agcgcgtcaa ggggttggac gccggtgcgg
     6601 acgactatct caccaagccg tttgcgatcg acgaactctt ggccaggatt cgcgccctcc
     6661 tccggcgcgg cgcgacggat agccccggcg tgttgcagat cgacgatttg gtcttgaatc
     6721 ccgcaacgcg cgaagtcacg agaggcggcc aacgcatcga tctgactttg aaagaatatg
     6781 cgctgctgga gtacttgatg cgccatagcg gccgcgtcct cacacgtccc atgatctctg
     6841 agcatgtctg gaatcaggac tttgacacgt tcaccaacgt catcgacgtc tatgtgaact
     6901 acctccgcaa caagatcgac cggggacgca cgaagaaact cattcatacc atccgcggaa
     6961 gcggctacat gctaaaggcc gactgacagg atgctgaaac agtcgatgtt ttcacccgcc
     7021 caaccccggt gcgccgacac gcaccctaca ccgagcttcg ttcttgcatt gctccgaggc
     7081 tcaccgtacc gaaacaagta cgccttgccc cctcgctcac tgcggcctcg ctggaaggcc
     7141 tgtttgagcc tcctgttggc ctattcgaaa tattctattc gtactgaacc gagtagattc
     7201 gcgttttgtc cggttcatat ttctggagcc tgctgatgcc actgcggctc cggctcaccc
     7261 tctggtacgg aagcgcgctc gcgctgattc ttatcgcctt ctccaccgtc ttgtacttcg
     7321 tgacggcacg cagcctgcgc gacgcgctgg atcaatcatt ggaggaaacc gccgctgccg
     7381 cggtgcgatc gctcgaagaa cgaggattcc tgccgctgat cgacgaggac gaattgatgt
     7441 cgcagttccc cgaactggcg cgcatcgaca agttttttca gatcttcagc ccctcgggca
     7501 ccatcacgat tcgctccccg aatgtgaaac aacatgagct gcctctcagc cgacgtgcct
     7561 tggaagtggc tttttccggc caaaccatct tcgagtccgc caagtatccc aaggagcccc
     7621 ctctccggct gatatcggtg ccgatcgtct atcgcggcaa cctcctttat atcgtgcagg
     7681 tcggaacctc gatggaatcc gttgatcaaa cgctgaaccg attactactg gtcttgttgg
     7741 tcaccatgcc cctggccctg gctgtctcgc tggcaggcgg ttggttcttg gcgggacggg
     7801 cgttgcggcc ggtcgatgcc atcacgctgg cggcgcaacg gatcgccgca ggcgatctca
     7861 cgcaacgttt gaatgttgcg gcctcccccg acgaaatcgg ccggctcgcc gccacgttca
     7921 acaacatgat tgcgcgactc gaaacctcgt tccggcagat acgtcagttc agcagcgacg
     7981 cctcccacga actccggacg cctttgacgg tcatgaaggg tgagaccgag cttgccctcc
     8041 gacgacctcg tgaagccggc gactacaccg tcgtcctgga aagcaatctg gaggaaatcg
     8101 accgcatgac ccgtatcgtt gacgaattgc tgttcctctc ccgggccgac atgggcgagg
     8161 tcaagatgga gcacttgccg gtcaagctgg aggcgctgct ggaggacatc catcggcaag
     8221 cgtcgttgtt ggaccaggac cgagagattc aggttgtact gggattagtc acgccggcca
     8281 cggtgttggg cgacgagttg cggctgcgcg aacttttcct caacctcgtc gacaacgcgg
     8341 tcaagtattc tcggccgggc ggctcagtga acatcacgct cgccacggaa gccggccaag
     8401 ccaagatctc cgttgccgat cacggtatcg ggatttctcc cgaagatcag accaagatct
     8461 tcgaccgctt ctatcgcact gacgatgcca gagcacacac caagaaaggc acgggtttgg
     8521 gtctcgccat ctgtgcctgg atcgcggaat ctcatcatgg ccgcatcgac gtccaaagtg
     8581 aagtcggaaa aggttccatc tttacggtca cgttgcccct cgccccgact ctccttaaca
     8641 acttctaata ccctcctaat cgtgctctca tcgcctgttg ttacgctctt cgtacatctg
     8701 aagagaagca actgtcttca atcggcttga tcacagcaag gaggtcatca tgagtcaatt
     8761 cgttcgcaga acggtcggta ccgccatcgc tgttgccgca ctcggcacag ttattttctg
     8821 ggggagccaa tccctgacat cgtcacatgc ctcgaattcg ccggctcccg ccaccaactc
     8881 cgccacgctt ccgatggcca ttccggcggc acagggattc acggacgtgg cgaaggccgt
     8941 gacaccagcg gtggtcaaca tcactacggt caccgtcgaa agcgtctcag atggtcgaga
     9001 tggtcaggat gaattgcgtg agcggatgga ggaatttttt cgggacccga acggtccctt
     9061 tggaccgcgc ggcttccgtg gaccgcaagg tccgcccgaa ccccgaggcc accgcggagg
     9121 cggtcaggga tccggtgtga ttgtctcgcc ggacggatac atcctgacca ataaccatgt
     9181 catcgaccat gcgcgcgagg tcactgtcac actcccggac aagcgggagt tcaaggggaa
     9241 gatcgtggga accgacccga agactgatct ggccgtcgtc aagatcgagg gacaaaatct
     9301 tccgactgtc gcctggggcg atgcctcgaa gctccaagtg ggagaatatg tgttggccgt
     9361 cgggaatccc tttggcctga actccaccgt gactctcggc attgtgagtg ccctaggccg
     9421 aggccgtatg ggaatcacgc agtatgagga tttcattcag acggacgcgg cgatcaatcc
     9481 tggcaactct ggcggtgcgc tagtcaatac gaaaggtgaa ctcgtcggca tcaataccgc
     9541 catcttctcc cagaccggcg gttatcaggg ggtaggattc gccgttccca ccagcatgag
     9601 caagccgatc tatgagagtc tgatcaagaa cgggaaggtc gtacgcgggt acctcgggat
     9661 cgggattcag gacctcaacc atgatctcgc caagtcgttc ggccttaaag acgcaaaggg
     9721 cgccttagtg agcgacgtga gggaggacag cccggcggac caggcaggcc tcaaacaagg
     9781 cgatgtgctc atgtcctacc agggcacgcc ggtcgaggac gccgtcgcac tgcagcggat
     9841 ggtcacccgt acggtcgtgg gaacaaaggt gcctgtcaag gtcatccgtg aaggacacga
     9901 gaaggaactg acagtcacaa tcggcgaaca gccggacagc gccaagattg ccaaggccga
     9961 gaccggagag aaagattttg ccttcgccgg tgtagcggtc caggacttgg atcgtgaccg
    10021 cgccaaagaa ttggggatca caggtaaaac gcaaggcgtg gttgtcacga gtgtcgaacc
    10081 ggacagcggc gcggaaaagg ccggcctgat gccgggcgac gtcatccgtg agatcaatcg
    10141 gcaaccggtc aaatcggtga aggacttcga gaaggtgtcc tcagcggtca agaaaggtga
    10201 gaacgtcttg atcctgatca accggcgggg cgcttcgttg ttcctcagcg cgaaggtata
    10261 ggctcactct ccagggatgg ggccttggcc ccatccctca cgtcagtcaa agacgacggt
    10321 tttcctgcca tacaccagca cacgccgctc aatatgaaag cgcacggcgc gggccaggac
    10381 gatttcctcg agatctcgcc cctttctaac caaatcctcc accgtgtctc tgtgcccaat
    10441 acgaatgacg tcctgctcga tgatcggacc ttcgtcgagg tcctgcgtcg catagtgtgc
    10501 cgtcgccccg ataattttca cgccacggtc ataggcctgt cgatagggat tggcgccgat
    10561 aaatgcgggc aggaacgagt ggtggatgtt gatgaccgga catcccgcct gcgcaagaaa
    10621 ctccgccgag agaatctgca tgtagcgggc catcaccacc aactccgcac gatgcgcatg
    10681 gagggccgag agcagctgct gctcctgttg gggcttgctg tctttggtaa ccggatagac
    10741 agcgaaggga atcttgaaca gctcggccca gctggcgcag gtgtcgtgat tggagagaat
    10801 gacggggatg tcgatgtgca gctcgtcccg tttatgccgc tggagcaaat ccgccaagca
    10861 gtggtcctgc tttgacacca aaatggcgac tctcgtcctg cggctggtcg gatgcacctc
    10921 gtaccgcatg tcgaacacct tggcaatcgg cgcgaatgcg gctgggatat catcgggagg
    10981 aatttgcaac ccctcggtcg aaaactccat tcgcatcagg aaatcgttcg tctcctcatc
    11041 cgtatggtgg tcagagtcga ggatattgcc gccgaagtcg tggataaatc ccgaaaccct
    11101 ggcgacaatg cccttgcgat ctttgcagtg gatgaggaga accacggcat ctttcttttg
    11161 agctgtcatc cctgtcgctc ctcttatgaa tggatagccg acgcgtagcg gaacaggcgg
    11221 gactctcaat cgaggagtgg attacagtac gatgtgcccg acgagatcgt aggtggcggc
    11281 ctcggtgatc tcgactttca cgaattcgcc agcgctggcc gttccctcat tgatatagac
    11341 gacgccgtcg atctccggag cgagtccttc gtgccggcct tcgagcaaca gctccgtttc
    11401 ctccgaaggg ccgtcgacga gcacgtcgag taccgatccg attttctctc ggctcttcgc
    11461 ggcggcgatc gcttcctgaa cggccaatag ctcgttacgc cgctcttcca tcagggatcg
    11521 atcgaccttg ccttcgaaat cgacggcggg cgtttgctcc tcatccgagt agaggaacgt
    11581 ggccacccgg tcgaattccg tcttctcgac gtactctttc aattcctcaa atgcctcttc
    11641 ggtttccccg ggaaacccga caataaatgc ggtgcggaac gtcacgccgg gaatacggcc
    11701 cctgatgcgc tccaccagtt tctcgatggt cgcacgatcg ccgaggcgat gcatgcgttt
    11761 gagcatccgg tcgttgatat gctgcaaggg catatcgatg tacttcgtga ttttctcttc
    11821 gtcggcgtag agatccaata actcatccgt gacttgctgg ggatagagat agaagggcct
    11881 gatccagcgc agctctttca ccttcaccaa tcctcgaagc agagtcacaa gtccgtggcg
    11941 catgccaaga tcaactccat aattgacggt gtcctgagaa atgagattga tttccttgac
    12001 gccttcagag gccagtcgct gggcttccgc cacgatcgat tcgacgggtc tgctccgctg
    12061 ttttcccctc ataagtggaa tcgcacagaa cgcgcagctt cgattgcagc cttcggcgat
    12121 cttcacatag gcactatgct gtttgccaag cctcagacga ggcgccatct cgtcgtacag
    12181 gtacggcggt ttgctgatcc acaggcgttg atggcgtttc tttggcgcaa ggaggtcgcg
    12241 gcagatgtcg gcgatcttac cgaactctcc tgtcccaacg accccgtcca gctcaggtag
    12301 ttccttgagc aaatcgcctt ggtaccgctg agccagacag ccggccgcga tcagtacccg
    12361 gcaggctcca tgttgcttga gcttgccatg ctcgagaatc gtattgatcg attcctgctt
    12421 ggcttcttcg atgaatccgc aggtattgat gataacgact tcggccttct gtggatccgc
    12481 cgttaagtca aagccgtcgg cgaccagcgt gcccagcatc acttcggagt cgacctggtt
    12541 ttttgaacaa cccaggttca cgaaaccgat cgtggttttg acgcctgaca ggggccggga
    12601 tttggcacga gtcgggcgac cgagtttgat caagggatga ctcataagaa gaccagtcta
    12661 cgagacgctt gaaaagcctg tcaattagcc ccttgcggcg aacaggccga ttcccctatt
    12721 gtgcggccat gattcgcacg ttccaaggca ttaagcccac aattcctccg tcctgcttca
    12781 tcgaggaaac cggcgtcgtc attggcgacg tggtcatggg ggagtcctgt agcgtatggt
    12841 tccacgcagt gatccggggc gacgtaaatt acattcggat cggcgatcgg accaacgtgc
    12901 aggacctctg catgttgcac gtgactcacg atacgcaccc gctgatcatc gggaacgagg
    12961 tcacgatcgg acacaatgtg gttctgcatg gctgcacgat caaggaccgc gtcttggtcg
    13021 gtatgggggc catcatcatg gatggcgcga tcatcgggga ggactccgtc gtcggcgctg
    13081 gggcattgat cacggaggga acagttgtcc cgcccaagag cctgatcttg ggatcaccgg
    13141 ccaaggtcaa gcgaccggtg acagagaacg agctggcctg gatcaaggaa tcagcggaga
    13201 actacgtgaa gtatgcgcga cagtacatgg gcgattcatc aaaagcgaag actgggtttc
    13261 acctctagca tttctcgccc ctcacgcctg acgccttgcc cccttaccga ttttgatgaa
    13321 gcagatcgga tcgccgacca gcgcagtggg gaactgttgc cgttccacaa tatagggaat
    13381 cctcttccgg tgacaccagt ctgcgatcca gacgacttcc tccgtcgaag tcgtcacaag
    13441 tcccggcagg ctcagcttgg gcatgcatcg atcgacgatc gattgaacca tccgtcgttc
    13501 tttctgaaag cgagcagggt tgaacgtgac cggatcggcc cgcccgtagg acaagggcgc
    13561 gaggtgtggg aaccgttccg gatcgttgag cacgctgact atccaaagat ggtcgaatgt
    13621 ccccttcgca atggccgcat ccttcccgac aaaccttacc gacgtcgtgc ggcaggttcg
    13681 attcagggat gacacttctg ccttccgaag attataaggg aagacctggc gctgcagatc
    13741 catcgtttcg accaccaacg ccggaagttc tccaacccct gccccgacat acagactgcg
    13801 cccgcctgga cgtaaccgcc tgagcaacgc ctctgcaacg cgcatcccga gacgacgaca
    13861 aggctcccgt ttagcgcgcc agaattgatt gcccccctca tagcagtaga tccctccgag
    13921 cgtccgatat tccagacgac caaagagact tgccactgca gcgcgcgccg actggctgat
    13981 ccggcggggc ttatttatgg tctgtaacat ccggcaagtt cttcgactga tgccaccact
    14041 gtgccgacaa ccccttccag aaacgtgaga tccaaggtgt cgatcgtgtc ggttggctga
    14101 tggtagtgcg ggtttctgaa gttggcggtg tctgtgagca tgacggcccg gaatccttcg
    14161 tcccaaaatg ccgcatgatc actccggcgg gcatcgggca acgtcatgcc cagaccagga
    14221 acagccaacc ccactgtgtt gagcgcggga gtgtatcgag cagcgttgcg ttcaatcgtc
    14281 gtgaccagtg actgggattc ctcgtttccg acgatcgcaa gaaagtttcc cacgcttgga
    14341 acggcaatag gcactcccgt cggtatacgc tgcgagcctt cgtcatggct ggcaaagccc
    14401 acacattcga ggataatggc accggcaagt tgttgatgag tgcgtttgag cctcgagacg
    14461 aacgcccgac tccccaggag accatgttct tcgagggaga aggctgcgag ccagaccggg
    14521 cctttcatcg ccgcgcgttt gagatggtat gccacttcca gcaagaccgc caatgcgctg
    14581 gcattgtcat ccgcccccgg agagttgaag acggtatcgt aatgtgcccc gatcagaatc
    14641 ggtgccggat ggtctttcct ggaaccggaa ggattcttga cggcaaggat gttccgatag
    14701 gtcttccccc aagcacgaaa ggcctgccgc gtcgtcgtcc atccacagtc agcaaactgc
    14761 tgggccagat accgagcgca gtcggcgagc gccttcggat ttccttccgg atgacgttct
    14821 ccggtcagtg catgaagatg gcgggtaact ctggttcgtc gattcgacaa gggggcagtc
    14881 aggtagtgaa tcctacgcgg ttatctctgt cccgatgccc ttgcgtgtaa acacttcgag
    14941 aagaatggcg tggggcatcc ggccatcgat gatgtgcgcc ttcccgaccc cctgttcgag
    15001 cgcgtccagg caggcgtgca ccttgggcag catcccttcg gtaatcgtgc ctttcttcac
    15061 catgcgctgg acatctttgc gcgacactgt ggaaaggtgg cggcccttgg catcgcgaat
    15121 gcctttgatg tccgtcatca tgactaattt ctccgcgcgc aaggccccgg caatcgcccc
    15181 ggctacaagg tcggcattaa tgttgtaggt attgccctcg cgatcggtcc cgatcggcgc
    15241 gatcacggga atgtagtgat cgccttggag cttgaacaga aggctcggat ccaccttttc
    15301 aatgtcgccg acgaacccaa agtcactgtc ggcgtcctct tcgtccagat ctcgctccag
    15361 gctttcggcc caggctttcg ccgtcagggg cttgctcaga atcagtccgc cgtcttttcc
    15421 gctcagaccc accgcgctcc cgccgtgacg attgatgagg tcggtgatct ccatgttgat
    15481 ttttccggcc agcaccatct cgacgatttc catggtggcc tcgttcgtca ccctcacccc
    15541 atgaagaaat ttcgattcga tgccgagccg atccagcatc ttgtcaatct gcggcccccc
    15601 gccgtgaata atcacggggt taattcccac atatttgagc aggacaatat cttgcgcgaa
    15661 ccgttccttc aacgacgatt ccgtcatggc gttgccgcca tatttgatca caatggtctt
    15721 gccgcgaaac gtgcggatat aaggcaaggc ctcgatcagg acattcgctt ttttgatgag
    15781 cttgttcatc gtcgactcct caggggatag cccggctcac ggctgggctc gacattacca
    15841 tgatgagcct ccttgagcac agagggaatc gtgccgaaac gtccgactca ccgcttacgc
    15901 tccttggccg ctcggtcggc gtgggttcgg cccgctctcg ggtgggactt gtcgtacacc
    15961 gccattaatt gatcagtcgc cacatgcgtg tacttctgtg tcgtgctgag ggatgcgtgt
    16021 ccaagcatct cctgaatggc gcgcagatcg gctccttcat cgagcagatg cgtggcaaat
    16081 gaatggcgaa gcgtatgggg gctcaccgag ccacccgtca gcctgcttga gtaccgtgcg
    16141 acgatccggg ccacgcttct gctggtcaat cgcccgcccc gatggttcaa gaacaccggc
    16201 gctaatcgca cgttgccgct tgtggacggc tgcaatgacc cccgataggt ctggatggct
    16261 tggatcgcga gatccccgat cggcacgatc cgttccttcc gccccttgcc tcgcaagcga
    16321 acaaccccct cgggctgatg gagatcttcg acattcatcg acacgagttc gctgacccgt
    16381 gcccctgtcg aatagagcgt ttcgagcaac gcgcggtcgc gcagtgaacc aatcgtgccc
    16441 tgatcgggaa atgccatcag cgcctctgcg tcgtctttcg tcaacacacg cggcagatgc
    16501 ttcggctgct tcggggtacg caaatcttcg acggggctcc ggctcaccag gcctgttcga
    16561 accaggtagc gataaaaact tcggatgcag gcaagctttc tcgccaagga ggtgcgcttt
    16621 tcgcccttgc ggtcgagcca ttggaggtag gcacgaatcg agtcggggga cagcgcggcg
    16681 ggatcaaccg gcgggtcggc gagacgggcg gagaggagaa aggagcgaag ctgccggagg
    16741 tccgacgcat aattgcgcaa agtggccggt gacgcgccgc gctcgaccgt gaggaacgtt
    16801 atgaaagccc ggatcgcgtc atccatgcgg catggtcctc gagggcacgc tgcgagatca
    16861 gctgtcgctt gcgctcctta tcacggaccg gagaagcgag tggcggaaag agtccaaagt
    16921 tcgtattcat cggctgaaaa tgacggggat ccgcagtggt gatatacgac agcaggcagc
    16981 catgcgccgt cgtcggaggc ggagcgacca gcggtaaccc ggcgagcccg cgcgcggcgt
    17041 tgatcccggc cagccctccc atcgccgccg aatccgtata cccttcaacg ccgaccaact
    17101 gccccgcaaa gaagagcgtc ccgcgggcct tgaactgcag ggtgtcgcgt agcaaccgcg
    17161 gagaattgat gaacgtgttg cggtgcaagc taccgtatcg aagaaactcc gcctgttcga
    17221 gtccggggat catgcgaaac acccgcttct gctcgccata ggtgagcttc gtctgaaacc
    17281 cgaccatgtt gtagcaggat cggtaggcgt tttccgtccg aagctgcacg acggcatagg
    17341 gtctgacgcc ggttctggga tcttcgagac ccaccggctt caacggtccg aactgcatcg
    17401 tctgtctgcc acgttccgcc atgacttcaa tcggaagaca ggcttcgaaa tagggggttt
    17461 tctcaaagtc cttcggttga accttttcag ccgcgaggag ggcatcgtag aacttgttat
    17521 aggtcgcctc gtccatcgga caattcaggt aatcgtctcc gcccttatca tagcgggatg
    17581 cccgaaaggc gatgtccatg ttaatggact ccgcatccac gatcggcgaa attgcatcgt
    17641 aaaaatagag atgattcgaa tgggttaact gggcaatggc ctgggacagt ttgtcggatg
    17701 tcaatggtcc ggtcgcgatg atgcacaggc aatcggtggg aatctccgtg acctcttcgc
    17761 gccgaatgcg gatgttggaa tgactttcga gtgcttgggt aactttgaat gagaactggt
    17821 cacgatccac cgccagtgcc gaaccagccg gaacccgcac ctcatcagcg accttgatga
    17881 tcaaagaatt cagccggcgc atttcctctt tcaggatccc gggagcattc agcacatcga
    17941 cggaccccag cgagttggaa cagacgagct cggccagccc gcctgtgttg tgcgcctgcg
    18001 tcatttcctt cggacgcatt tcgtagagcg tgacccttgc tcctcgattg gccgcctgcc
    18061 aggcggcctc ggatccggct aagccaccac cgataatcac aatgtcgtcg cgcattcagg
    18121 aagaactcca aggaggatta ggcaagaagg aaatgcattg taggaaccaa attttcggga
    18181 tgtcaaggca gaccttgccc agaatagcat gccgtaggat ccggaaaact ggtacgagct
    18241 gatcgagcca ctcctcggtc tacttgccgc cgcaagatcc attgatggtc aagttgaacc
    18301 tcgccgaaga cccatgctag acttcggcct cgtgggcgat tagctcagtg gcagagcgct
    18361 tccttcacac ggaagaggcc acaggttcga tacctgtatc gcccaccatc ctccgaaatt
    18421 ccctcataat tcaagcctct agacattact cccgaacgat tgcgcaaacc caccgatttc
    18481 tggacagaag ccggaacctc ggctacactt cactcatcgg aacatacaac gcaccgataa
    18541 tagaggtcac ctatgaagat acgagcatgt gcaacgggaa tcctactgct gacaatggtg
    18601 gtaggttgtg caggaaaccg gcagcaaagt atggatgcgg atgggaacta tgtggctccg
    18661 gccagcatct acagcgtgat ggacagcacg tcgttggtct acaccgatgt cgctgccggt
    18721 tctccgctga atgacaaccc ctggcgctgg ttaggatttc tcttgcaccc agtagggcag
    18781 gtactcgatt atggcttcaa ccggccggtc tattcactca cagggaaaat gccctatttg
    18841 ttcggctata cgagcgaaga tgcgatgctc gacagtacgc gccagtagtc tgacgatttt
    18901 cacttgatcg taattcaacg cccgctttct tgatgaaagc gggcgttgtc attttgagac
    18961 ctggcaaacc tcttcccatt tctgtatcct cctcaaggtc atgctcggaa tgctcaaaca
    19021 ccgggggtgc aagctgggac gattgttact cgtactcgcc ggtgcactta gcctcgccgg
    19081 tctcatcgcc gcttgcgaaa caccctcgcc gcgccaaaat ctgcctcctt ccgagactga
    19141 cctggtactc ctcaagccgg gaagcctgtg tcagcgtaaa gccaattttc ttcaagccca
    19201 tccgtcgccc acggtaaagg tctgggggac gggacaggaa ttgataatcc cagctgatcg
    19261 aagcccttcc catggagacg aatcctattt ttttgacgag gatggcacca tggttggagc
    19321 gctgttcact ttttcgtctg gccttgactt ggccccctac tcggttcttc gccacaccct
    19381 gacactcctc aagccgtctc tggagttcta cctgaacgtt gccaactttg ctgcgaagtc
    19441 cgacatggat tccagttccc tttatgaaac cggtgacgag aaaacgacca cacaatattt
    19501 ggtgatcggg gggcatgaac atccggcgtt gctccaagcc tctatcacca tcgatcccta
    19561 tgtccgcctg ttctccccct atcgacggga atttttaacc cggctgcggc aaccgggcgg
    19621 tcaaaagcag ggccagcagg tcgatagtca aggggccgag gataaagaac cgttcccctc
    19681 tctccaacaa tttgcacgcg gtcagacggc gcaactgtcc tattgcggcg cgcaacatta
    19741 cgagatcgcg gccgacgcct atcacaaagc gatcacaagc ggatttacca acaagatctg
    19801 ggttgcggaa gtgcaccaca aatacgggca gaccttattc ggactcggtc agtacgagaa
    19861 agccaaagcg gagatgttgc aatcgttggc aatcaggccc aacactcccg agattctgaa
    19921 caatctcggc tctgtccaac aaaagcttgg cgagaaggct gcagcgcagg cgtctttcga
    19981 aaaagccatt accctgagac cgaactatgc cattgcccgc ttcaatctgg ccgaggccgt
    20041 cgaatcgacc aatcccaaac gagccatcac ggaatacgaa acctatttgg ccctggtgga
    20101 aggtattccc gaagaggccg accgcatcgc cctggtccaa cagcgcctga aagcgctgaa
    20161 gcaccaatag ggcaccgacg ctcggggccg gtgagtcatc agacagaaca gtcgtgcgag
    20221 tggacgaaac ctatcggcga atcttctctt cgtgttcttg gagggttttg caggcgatac
    20281 agagcgtggt gacagggcgg gctttcagcc tttgataggg gatctcttcc tcgcatcgtt
    20341 cgcagatgcc ataggtgttc ttcttcattc gatccagcgc ttcatcgatt ttcttcagga
    20401 gcttctgctc acgctccttg atgcgcatcg taaaattctg atcgacttca gcggaagctt
    20461 gatcgctcac atccggaaag gcctcctggc cttcacgttt ggtcaggcct tcactcgctt
    20521 cctccagaat cgccgcacgc tgtctctgaa ggtctcgata aatcgcgctg tactttttgc
    20581 tgatgacggc agtgctggct tctcctttgg aggccagaga ttttcccgat gttcgtgctg
    20641 tgggcttcgg agatgttcgt attttcatcc ttcaccagct aattccggat acaatgcaaa
    20701 acaggggttg gactataaca ggggcttgga aagcgggtca atgggagaga cggccccctc
    20761 ggtccggatt actagagctc tcggcggcct tcgatggact tcagcagcgt cacttcatcg
    20821 gcatactcaa tgtccatccc caccggaata ccataggcga ttcgcgatac acgggcgccc
    20881 agcggtttca gctgattcgt caaatagatg gcggtggctt ctccttcgat tgtcggattg
    20941 gtcgccacaa tcacctcttg gatgccgccg agcctgacgc gatcgatgag ttcttccgct
    21001 cgaatatcag acggtcccac cccatcgagc ggtgaaagtg cgcccaacaa cacgtgatag
    21061 agcccgcgat acccgccggc ccgttcaatg gcatagagcg tgctgggctc ttccacgacg
    21121 aggatcttcg ttctatcccg ttttggatcg agacaaaact cgcagagttc cccttcggcg
    21181 atattgcggc attgctggca aaacgaaagc ccgtccttca cagcgcgaat ggcttccgcc
    21241 aaacgcagag catcctcccg ctccgctttc aacacatgaa acgccagtcg ctgtgcagtt
    21301 ttctgtccaa cgccgggtag acgaactagc tctcggacga ggcgggcgag taatccctgt
    21361 tgatcaacgg ccatcagtga tcactcttca aaagagaccg ggcacgttca agccaccggt
    21421 gaccgctttc atttgatcag ccatcatctg gcgggctttc cgcaaggcat cattcgaggc
    21481 cgccatgatc acatcttgaa gcatgtcggc atctccgctt ttcaccgctt ccgggtcgat
    21541 cacgatgctg accagctcca tcgctccatt ggcggtgacg gtgaccattc cgccgccggc
    21601 tgatcccgtc accgttttgg tagcggcctc ttcctgcagt ttggccattt gtgcctgcat
    21661 agcctgcgcc tgcttcatga tgctcgacat atttccgaat gggtttttca ttcctgcacc
    21721 tctttctgat tcgacaccgc gcggacatcg gcaaggtccg cgccaaaaat ttccagcgcc
    21781 tgcttgacga ccggatgggc cttcgcccgc tcaaaaagga ccagcttttg atcctgctcc
    21841 ttcgccgctc gaatctgtgc catggtcgga ccaggaggat cggcttcggt caactcgacg
    21901 atgcggatcc ggacgggttg accactgatc cgctcacaga gcgaagcaag ggtctggata
    21961 ttgtcgggct tctccagcat gcctcgagcg accgtcgcct gtttggcgaa tcctatcgtc
    22021 acgacattcc cttcaatcgc gaccaagcgc cccatttcca ggaagggagc gatgttagga
    22081 aatgcatgag agacgtcgtc ttggaactgt tcccaattca gcgcgactgt ttgcactatg
    22141 cgaagcgagg tggttggtgc agagtccatc ggtttggtcg gctctgcaat cgcggatttc
    22201 accgtctggg ccatggcacc gcctcccgtt ctaggcacag gaccggatga gggggaaggt
    22261 ttcggcacag gcgtcgtcgg tcgagagggc gccggagccg cagaacgttc cggacttgtc
    22321 gccttgtctg agcgtgaaga ggaggggaca ggtggacgct ccgatggagt cgctcgaacc
    22381 gctggaccag ccttctctga ttcctgcaag aaaagtctcg tggcctttac tgctgcagtt
    22441 tccagcacaa atcggggatg ggcgctcagc ctgagcgcgt cttcggcctg ggtcatcaga
    22501 gcaaagattt gctgaagctg ctcgggcgtg agcgaaccct tgtcggctgc gatttgcgcg
    22561 agatcctcgg acgaggcctc gatcaacttc ggccattcct gctgcgacgt gaccgacacc
    22621 acgagcatat gacggagata ttccacgacc tccgcacaga atgcccgcaa atcgtgccct
    22681 tggtccaaga gctgcgcgag tacgcgaagc gctgccgcgc tgtcctgcag cgtaatggct
    22741 cgaatcatct cgcgtagcag ttcctgcgga acggcaccaa gtaacgcctc gagatcctcg
    22801 tgcacgaccg atttcccgcc gaaggctacg gcttgatcga gtaagctcag tccatcgcgc
    22861 atgctgcctt cgctggccct ggctagggcc gtcaggctcc gctcttcaat cgcgatgccg
    22921 tcctgttggg ccacatggcg cagccgatcg atgatctcgg tccgcgcgat ccgacggaag
    22981 ttatagtgct gacagcgcga aagaattgtg gccgggattt tgtgaatttc tgtcgtcgca
    23041 aaaatgaaga cgacatgagg aggaggctct tccagcgtct tcagcagcgc attgaacgcc
    23101 gagttcgaga gcatgtggac ttcgtcgatg atgtagacgc ggtatttccc tcggaacggc
    23161 gcaaacttca cgttttcgcg gatttcacgg acatcgtcca cgctcgtatt ggaggcgccg
    23221 tcgatctcga tcacatccac ggaagtccct tgggcaatct cctgacagtt ggcgcaggtg
    23281 gcgcacgggg ttcccgtcgg cccctgttcg cagttgagcg ccttcgccag aattcgcgcg
    23341 accgtcgttt ttcccacccc gcgcgttccg gagaacaggt aggcttgggc gatccgtttc
    23401 gtgttgacgg aattcaccag cgtctgcacg acgtgcggct ggccgatcac atcgtcgaag
    23461 gtgccgggtc ggtatttgcg ggcggagacc tggtaatcca tatgacgtca ggcgtaagga
    23521 gtaaggcgtg agggggtgag atgaatgatt cttggaatcc gtatcacctt acgcctcacg
    23581 tttcacacct cacgcattac ataagcgggg ccaggtgatc ctgcggcacc caggactttc
    23641 tgcttaccgt tgcttccttc cggacctggc ggggttcaca gggttctgtt gcacagggcc
    23701 cagccccttc gttcacaagg caatagctcg aatgctctga acacttgtgg tgcttatccg
    23761 cccgcaacaa tcacgatact gcccaaaaga gcgggcggtt cacacgaagt gtggtatggc
    23821 ggagagggtg ggcattcgaa gccgcgctcg cccgactgct cgcgttccta cagagggggc
    23881 cagccccctc gtatgctccc cgcgcggggg acatgtccct cgcgatcccc ctgttcgaat
    23941 cccaccctct gctcgctgtg aaactcagtc gtcagcactc atagctcagc actgcttttg
    24001 atggcggaga gggtgggatt cgaacccacg gtcccgttgc cgggacgcat gctttccaag
    24061 catgtcgatt cgtccactct cgcacctctc cgcgacgatt ttcgagaagc ggcatcttaa
    24121 catgcaccat agggagcggc aaggtgagcg tttgaccctt ccgcacggct ttcctatact
    24181 gccaatgact cgaggggagg tgcagaatga aaatcgtcat cgggctgata gtggtcattg
    24241 tgctcctggt cggcgggatt ctctcgctgc cgttcctcgt cgatttgaac aagtatcaag
    24301 accattacaa accgctcatc gaagaggcct tgaaccgcaa ggttcaactg caagacatcc
    24361 ggctgacgat ttggccgcgc attggtgccc gcgttgccgg ctttaccgtc ctcgacgatc
    24421 cgtcgtttag ttcaggtccc tttgcgtctc tcacctctct ggatgtcggc gtgaaactcc
    24481 tgccgctcct gagcggaaag gtggaagtgg aggagatcac cctccgcgaa cctgtcttaa
    24541 cggtcatcac gaacaaaaac aaggtgatga acgtctcgac tatcggtccg aaaacgccga
    24601 cgcctgccgt ggaatcaggt gctccatcgc aatcccatgg agacccgctg caggccctcg
    24661 ctctcttagc ggttgatcaa ctctcaattg acggcgggag actcacgtat cgtgacctct
    24721 cgacgacgcc ggtcaccgag tatcagatag agggccttga gctcgcactg aagtcagtcc
    24781 gtctcggcca ggcgccgaca ctccatttcg ccgccacggt acagccctat aacctccccg
    24841 ttgcgctgga cggaagcttt ggaccactcg tcgaaagatt ggaggtgaaa cagtacgatt
    24901 tcacgctggg attcggcaaa gtcgcgctgg gattgaaggg cgcgctcctc gatggaacgc
    24961 tgaatgcaac gctctcatcc ccctcgatct cgaccgcgga cttaccggtc acgctgcctc
    25021 tcaataaacc ggttcagatt aaagaccttc gtgtcgtcgc caaggcgcct tatccgctca
    25081 aacagggagt ctcgtcgatg gaactggcag acgtcacgga tctggacctg gcgctggtga
    25141 tgggcaattc gtcgctgaat gtcaaaggca cggtcctcgg cggccacgcg aagctgacac
    25201 tcgcctcccc ctccatcaat accgccgatc tcccggtcga caccggtttt cagaaacccg
    25261 tcgagataac acatctggaa gcgaatgcag acctgaaggg acaggaggct cggttaacca
    25321 acttatcctt ccggttgttt aacggtcagg cgaaggcaca ggggggaatg tctacggcaa
    25381 cggcgacccc gccctttaac ggcatggtca cgatacaagg actccagctc ggaccagtgt
    25441 tggacgccgt gagcccggac agcaaagtgt caatcagcgg aacagcggcg atggacattg
    25501 ccgtcgcagg ccgcggtttc tccatgcctg acttgaccaa aaccttggag ggaccgggcc
    25561 acctggaagt caaagaggga aagatcgaag gggtcaatct gatggaggaa gcggtcacgc
    25621 tgttgaaagt tgcgggcatc tcgctcgacc gggcaaacgc cacggccttc tccatcattg
    25681 agaccgattt cacgatcagg caagggatca tcaatgtgca gaagctcctg atggacagtc
    25741 acgacttcca ggcgaccgga aacggaacgg ttggattcga tcaaagattg aatctcgcca
    25801 tgaatctgaa tctctcccaa acgttgagcc aaaaactcgc cggttcgtcg ccgatcgcca
    25861 agctggcgct gagggatggt cggctgagac tcccgcttct cattaccgga acggtacaag
    25921 ctccttcgta tagactagac gccaggggtc tctcgggcaa ggtgcaagaa caggtgcagg
    25981 aaaaggtcaa agaggcgatt ggcggtttac tcaagggaga atcgaaacct tcggacttgg
    26041 agaagcaggg ccaagacctc ttgaagggac tattagatcg ataagcgtcg cagggaatcg
    26101 atttctgaag gccaaatcga agggtagctc ttcatctcgt tccggcgaga ttcgcttcgc
    26161 acaaataagt tttgactcaa ccaatcagcc ccaacactag gcgccgagcg gcaccacttt
    26221 tgctgatagc tacgaggccc gcgcctcagg attgcgattc ttagcaattg cagcgacgtg
    26281 gctattcagt tatacatcta cggcattttc tcgaggctga catcccaatt gcccgcaatg
    26341 tcgccacttt gccgggataa tggatcatca aatacttgcg gttgctcccg taaacggagg
    26401 gttcgatgat acagacagtc ttggttcggg ctctgttaac gatgacgctt ctcatcggac
    26461 tggtctcaac agttgaagcg caaacagtgc ctcccgcgcc ggaaccggct ccgctcgaca
    26521 tcgtgacgtt aaaggatgga agcatcctct atggtgaggt gatcggaatg gagagcggcg
    26581 tcctacacct caagacccca gccagccctg acaatgtagt gaaggtgaaa tgggagagca
    26641 tcgccaagct tgccgtgaac cacccgctcc cgtttcatct caaagaaggg tccgtgctga
    26701 tcggcacggc caccgagggc cccgccggaa ccatcatggt gcaagctgag ccgctgaaag
    26761 ggacaatgga ggttccgctg gattcgatcg ctgctgtgaa tcccatcgtc caagccccgg
    26821 ttatctatca aggcaccctg accggcggct actcgcaagc aaccgggaac agccacctga
    26881 ggaatgccag cctcatcgga gacttcgtgg ctcggggtga gcagctccgc ctgactctca
    26941 acggtcgata cgtctacggt gataaggaca acgagctctc tgctcgaaac gcgcgcggca
    27001 caatcaagat ggactttttt atcaccaaac gctttttctg gttcgtgtcc gcctactttg
    27061 agaaggatac ctttcaggac cttaaattgc gaaccgccct ttcatccggt cccggttatc
    27121 aaatcatcga aaagggtgat tacaacagcc cctggctcaa agacctcacg ctctacttcg
    27181 acgccgggct tgcctacttc aatgaggact ttacgaccgc tcctgatgaa acgtccatgc
    27241 gcggcaggtg ggcagtcagg ctcaactggc cgatgttgga tgatcgcatc acggtctatc
    27301 actacacgga attttaccct tcgttagaga acaccaagga ttattatttg acgatggaca
    27361 acggcgtccg tttcaaatta atcgacggtt tcgtgagcgg catccaagtg acgacccgat
    27421 ataacagcaa tcccgcgcag ggcaccggag atacggacaa cttgtacctc ctgacccttg
    27481 ggtacagctt cgacacaacc aggaaacgtt aagcgcacag ttcacccata tcctctggaa
    27541 aggaggaatc gtatgctgaa agaatttcga gattttgcca tgagaggcaa tgtcatcgac
    27601 atggcgatcg gtgtgatcat cggaggggcc ttcggaaaaa tcgtctcttc gttggtcagc
    27661 gacgtgatga tgccacccat cggcttgctg atggggaagg tggatttctc cagtctcttc
    27721 atcaacctat cgggaacacc tcagccgtct ctgacggcag ccaaagcggc gggagctccc
    27781 actatcaatt atggggtctt ccttcaagcc acgttcgact tcatcatcat tgcgttcgta
    27841 atattcatgc tggtgaagca agtgaaccgg ctcaagaagg aaccgcctgc tggacctccc
    27901 acgacaaaag attgtccgca ttgtttgtct accattccga tcaaagctgt gaaatgcgcg
    27961 cattgcacat ccactgtgta gatgagagat ccgttcgtca ggtacgtcaa ccaaaggaga
    28021 tgatcatgat gagatggttc aagaccgcag ttctcgcaac cggccttctg gccagcgcga
    28081 gctatgcatc ggccgtggat ttaggggcga tctgttgtga cggcggctat gaataccgcc
    28141 accccaaccg ttgggaacta gaactgcagc gattgaagga tgaactggcc gcagcccaga
    28201 aacagctggc cgatcgcgat cgggagatcg cctccctgcg gtccggctcc aatgaagccc
    28261 tccgatcggc ccaagctgat ctcgaacgat ccaagacacg tatcggggat cttgagcgac
    28321 aacttgctga tcgggatcgt gagatagcct ctctccgctc cggtgcgggc gactcctcga
    28381 aactggcaag ccagttgtcc tctacacaag gagaacttgc tcagtctcaa gcacggacag
    28441 ccgaacttga acggcaactg gctgcactcc aagcttcaca agccgggctt caggcatcat
    28501 ctggggataa ggatcgcatg gccgctgagc tcgccgcggc acaggcacaa ctcgcagcgc
    28561 ttcaagccgg cgctggagac aaggacaagc tcgcggctga atgcgctgct gcgaaacagc
    28621 gaattgccga actcgagagg cagctggccg accgggataa agaactcgcc ggccttcgcg
    28681 gcgatctctc cgctgaaatg gccaagttga aagaggctca gcgcgggctt gtgcgtgcac
    28741 ttcgtcctca gatcgaaaaa ggtaccatca ccgtcgatct gaataacgag cgcctcctga
    28801 tcaatctcgc gtcaggctat ctctttggtt ccggcgaaga tcaactcaag ccggcgggag
    28861 ccgacgcgct caagcaggtg ggaacgatct tgaaagagtt cccggaatac aaagtcgcgg
    28921 tggatggtca taccgataac cggccgatcc gcggctcatt gaagaagaag ttccccacga
    28981 ataaggaact ctctgaagcc cgtgcggcca acgcggcgaa ggcgctcgcg gagggcggtc
    29041 tcacctcctc aaccacgaat ggttatgcgg acaccaagcc cgtggaaccg aacacgaccg
    29101 aggcgggtcg agccaagaac cgccgcgtcg aagtacgtgt gacacgctag aaacccctgg
    29161 gcacagctgc cctatcacca gtgacggggt agcggattct ccgctacccc cgccacatca
    29221 taatagtgaa gaggtgttct atgaatcaga tgatcatgat cctatcaatc gcggcagtct
    29281 cgatcgttta ccagaccaat gcaggtgcgg ctgattctac gcaacaactg ccaacactgg
    29341 gcggtataaa atcagccgtg cagcaagaag ccaatcaaga gaagcaagct gccaaggatg
    29401 ctgcccaggc tgaaaagtca aaggccgagc aagccgtggc agctgaaaag cagaaggcca
    29461 aggatatcgt tgagagcaag aaatcgaagg ccaatgcgaa ggcggccgaa caacagcagc
    29521 gcgcgaaagc cctcgccgcc gagaagaagg caaaggccga agcgatggca gcagagcaaa
    29581 aacaaaaagc gacggataag gcgcagagcc aggtcgacca gaccctgcag aaggcatccg
    29641 aaaagaccga tgcctcaatg ggaaaagttg atagtaagtt ggacagcctg ctcggcaaat
    29701 gacggtagga gagcaatcct ggggacgcat cttatgatgc gtcccccttc gctcacatat
    29761 cacagatggc agccactctc gtcgcgattc cccgcaattg attcgcctgc aacagtagcc
    29821 agaattttgt agtgcccaag ccccagttcc ttcccgaacg cctcacccac gaggacatcc
    29881 tgcacgtgct ggtgatccca tgcgcggaag ctggatttgc ccactttgag cccgtcgaac
    29941 tcatggcctt ccatcgcttt gatcaacgcc gtcgtctcgg tcgtaccggc ccgttggatc
    30001 gcttccagaa tctgcgtcat ggccgcatag ccaagatagc agcgcgaggt cggcgtatga
    30061 ttgtacttgt cgatgacccc ttggataaat cgtctggagc tatctgtatt aaccttgggg
    30121 tcccagataa gcccccagat acccgcattg ttggcatagc ccagcggtcg cccgatttgt
    30181 tccccgccga tcatcccacc gatgccaatt ttctctttcg ccaattccaa tttcgtatag
    30241 gctttgaggg cgtgaatcag atcccacccg tacaagttaa gcagaatcaa cgtcgggcgt
    30301 ttctcctttg cggcagtaag ggctgatgaa aattccgtag atccgaacgg catcagcgcc
    30361 tcacccacca tctcgactcc gagggactgg ccggctgtca ccatggcttg tgccattgcc
    30421 tttccgtcca acgtactggt cgtaatcatg taccacttgt tgccgtacgc cttcagcaga
    30481 tacggcgcta ccgcccggga catcatggag gcattgggca taaacacaaa ggtgtggcga
    30541 ttgcaggccg cgccggtgag ttccggaagg tgtgagcccg tcaccatgaa gagcttgttt
    30601 tcttgttggg cgagcgcgct gacggcgagc gcacaatcgg cattgaacgt ccccaacagg
    30661 acatcgacac ggtcctcctt aatgaacttc gtcgccactt tcacggccgt ttcagggttc
    30721 gactcatcgt ccgcctccag aatgacaacc ggccgcccca gcacaccgcc attcttgttg
    30781 aagagatcaa cggcgacgtt cgctccatgc acatcgtgga tcgacgaggt cttatacgga
    30841 ctcgagaggg gatcgagaat cccgatcctg atcggttccg cggcatgcgc cactcgtgcg
    30901 gcgggcaaac gccccaggac ttggccgaga agatctccca ataacaatgc accgccggtc
    30961 actgccgaca cttgcagaaa acgtctccgc gacatcttgc ccatgtcatg ccctcccgtt
    31021 tggatccatc cgtcacccgt tgcgtaggtt accgccaacg actgtgagat agccagaaga
    31081 ctgtcagcgc ctcaaccgat tagacgtttg cggcacgcaa tgcagcttca agctcggcga
    31141 tgcgtcgctt caatgggccc actaattctt cgatctcctc gagtgggtac cgtggtgtaa
    31201 tgtactttgg gcagttccag tcgaacgaga caacgtcgat gaagaccagc cgctccacac
    31261 gcgaccgcat cttcggatcg gcgagctgcg tgacgagttc ctgatgggct cgggcatctt
    31321 cgatgcgggc atggccgagt atcttcagcc gctcgcggtg cttgtagtcc ataagaaaga
    31381 gtgccacgcg gtcgttcacc gagacattgc cggtgctcag cagttgacga ttgccggtat
    31441 agtcagcaaa cgccactgtt gtctcgttga tcacccgcaa gaatccagga ggcccgccgc
    31501 gatgctgaat atacggccat ccgctttcgc tcacggttcc caggtagaag ctgtcccgtg
    31561 ccgcaataaa ttcggcctcc gcccgggtga gcggatcccg ttcgggagcg tctttgattg
    31621 tcgccgcacg tccataatag tgtttctggg cgcggcgcac ggcatctgta aaggtcagat
    31681 cgagatactt cgtcaccatc gttgcacccc agtggttgcg gaatccatga gtgatcagaa
    31741 ttccgtcgtt cgcggtctta caagatgcga ctcaaaatac gaccgtgttc catccctcgg
    31801 ccaggtaacg gcggagactc aagagaccgc cagtcccggc caaggtgtgg tctttcatga
    31861 tcggcactcc acaggacttg ataccctccg tcgcctcaaa gacctctgca cagccacatg
    31921 aggccccttg cacgacatca cgaaccgagt tgtagaggcc gttggccgga tgcgaaagct
    31981 tggtgagttc agcaggccaa cgcgtgccgg ttccattgaa cacgacggcc acctcctcgc
    32041 ccttctgttt gcactccgac gccagggcaa gcgcattaaa gacacgcccc agtgcttcgt
    32101 ctgaaccgtt cttcggatcg gacatgatga tgatggcggt tttcatgggt cctcctgcta
    32161 cagaattgtt gtgtggttgg taaccatctc cgggccctcg attagctgct cgtccggtca
    32221 tttcttcctg ccggaatata taaccgtcta ataattgttt tgacagttat acgcctggca
    32281 ggctaacctg tcaagtactc ttttgccggt atactcgtgc gaggggaatg acatgacaac
    32341 ggggcacgtg cgcacacagc agccgttcct gtttgtgggc aatcatctat gcttggactt
    32401 cgtgaacaca cagatgatcg tgaggggtaa accgactgat cttctaggag gatacgagga
    32461 tctcgtcgct tggttggttc aagcaaagat ggtaaatgca gcccttgcca cagcagtcat
    32521 gacacagtgg aatcacaagg accaggagca gctctttgag gagggaattc tgttccgcaa
    32581 gattttgcga gaaatggccg ggcgaatcgt ggctcgcaag accattcctg gctcggtggt
    32641 cacatcaatc aaccgacttt tgtcccggtg ccccggctac ccgcaactcg tctataccaa
    32701 gggacgattt gaacgacact tccagtcttt ggccacgcag aaggacggcc tactggctcc
    32761 actcgccgag gctgccggcg acttgctctg ttcaggcaat ttgtctctgg tcaagaaatg
    32821 cgggaatgca gcctgtatct tatactttta cgatacgacc aaaaatcaca cccgacattg
    32881 gtgcagcatg cagttgtgtg gcaatcgaat gaaagtggct gcccattatc acaggaagcg
    32941 ggacaatcca acgcattgaa cagttgctgc gtggcgatat gattatggta tggaaagaac
    33001 cggccgcagg cgaaggcctg cggccggtca tacgttctag ctgaattgat cccaggtgtc
    33061 acattgcttg atcgcccaaa acaccgcttc cttcaatttc tttagcggaa tattatcggg
    33121 atctcgcatg gcctgaagct tcctgtctag atcgtacagc ggttggatcg atcgtttatc
    33181 tggaatcctc cccaacgacc aggcaacttc tgtcaggaca acccagtccg tcttggggtc
    33241 ttggagcttc gccaggagtg gaggaacgac cgaggcatct ccatgtgtcc cccccagctg
    33301 tcccaaaccc ttcgcggcag ccgcttgcaa ttcaatctga ggagatgtgt tcatgatatc
    33361 gacgaggagc ggaatccctt ctttgccgag attccccatc gccccaatcg cctgcttgct
    33421 gagatcccgg tcctgcaaga cttgtttcag tttgggcagc gcttcgtcgg cctgcatttc
    33481 gcccaagagc gaaataatcc tgaacttttt ctcctgggga gtgtcggcgg cgtcgagtaa
    33541 cttgagcaat cgttccggat gtccccattc agccgccagt aggagcggga agtcgctttt
    33601 ctccagttcc tctttcagct tgcttgcagc aaaagccccc ggatcccccg cgttcgccga
    33661 tagagccgct tgcccggcat cttcaaaatt cgttcgtact tctttttgcc atttgacctt
    33721 catgtccccc agccgataag tcaattgaca ggccggtccc ttccagagtc ttgatggagc
    33781 atcggggagg tcggcatcgc caccggccgc cgtcgtccct tcccatatct tacgctgctc
    33841 acatttcact ttgaagacgg catcgtgggg cttggtcggg tcttgcacgg tcgtgtaccc
    33901 cagctcccgc agccggcgag cgacaacttc cgcaatcggg ccggcatcgc cggccccctt
    33961 gtcggtcagg acaatgacat ccaccagcac gatctggatc ttatcgagtt gggccttctg
    34021 ttccgccgta aagtaatcgc gataggccca tgcggagaga ggaatgattg cgataatgat
    34081 gtaggccagg atcaaacgca gtagggtcac acgcatggcg cacctcctca ccagttcgcc
    34141 ggcaactgct ctacgtatag ctcctgaatt ataccggtca cggtcaaccg acgctatcat
    34201 gtgggtcatt atacatcccc tgctgatcgc gttgcgcaaa gccaaattgg cccgtgtcac
    34261 ctcatttcca tctgctcact ctggtgcgac gttccgcgcg tcactgggcc ccttcatgaa
    34321 taactggatc ttcgggcccc tagtggctgg aaaccgcctc tatccccgca ggagtatggg
    34381 ttgacagtgg tgctcatcca acggtacaaa ataccgacag gaacgtgtat tttcgcttgt
    34441 ttgctgactt ggctggaaag gactccagcg ctccccgccg tgcgagagtg gcggaactgg
    34501 cagacgcgcg agacttagga tctcgtgggc aaccgtgggg gttcaagtcc cccctctcgc
    34561 accagaccac gacgcggcac gaaacacgct gtttgtgccg acgctataaa ggaacgactc
    34621 gacacatgaa aatggaagtg acagaactgg gcccgatgaa gcgtgccctg aagatcgaag
    34681 tgccggccga ggaggtggct caacgattcg ttcgcgccta tagcgagctg aaccgtcaag
    34741 tgcatatccc ggggtttcgg ccaggcaaag ccccagtggc gctcctcgag aagcgttatt
    34801 cgaaagcggt tgaagaggac gttgtgcgta gcctggtccc cgacttctac gaccgtgccg
    34861 tccgacaggc cgggatctct ccggtccttg tcgagattcc cccgctggag cgtgtgaaga
    34921 tcaagaaaga tgcaccgttt acattcacgg caacggtcga gatcaagccg aagatcgaac
    34981 tccgcgacta caaggctccc aatcctatct ccttaaagcc ggacaaacgc acggtgactg
    35041 atgaacagtt ggccagaggg atggaggtgc tccgggaaca gcacgcacgg ttggacgctc
    35101 ccgctgcggg gcataaggtg agtgaaggtg attttgtgat tctgtccctc gaagggcgcc
    35161 ttgacggaaa gccgctcgaa ggctccaaga aagaaggtca tctgcataaa gtgggatcga
    35221 aggcgaccgt gctggggctg gaaatcgact cccacgtctt gggaaaaacc gagggggaga
    35281 cggtcgagat cacccagcca tatccctcga gtcatcccga cgctcgcgtg gctggcaaga
    35341 ccgtgatctt cacgatcatg gtgaatgcgg taaaggtcaa gaagcttcct gacctggacg
    35401 acgaattcgc caaggactgc ggcgcctaca attctctgga tgaattgaag gataaattgc
    35461 gcggcgagct ggaaaaggcc ctgaaacgag agattgaaga tacttacaag gatacgatcg
    35521 tgaagcggct ggtggaaacc catcacttcg atctcccgga aacgctggtg gaacgcgaat
    35581 tggaagcaat cattcgtcaa catatgcaac agcgccagcg cagggcaggc gggggtgaag
    35641 gtgctgcggc tcccgacacc gaggatctca cgaagcttcg tcaggacaat cgagatgaag
    35701 caggccggcg ggtcaaggtt ggcttgatcc tggaggccat tgcggagaaa gaaaacttga
    35761 ccgtgacgca agaggacctg aacaacgaaa tccatcggtt ggcgacggaa ctcaagatcg
    35821 cccaggccga tctcgtcaag atgattcagg ccggcggcca agactcgatc gacgaattac
    35881 gcgcgcggat cctggcggaa aagtccctcg atttcgtgta tcgcaatgcg gtgattcaag
    35941 gatagcctcg gcggcagtca cgtggttgcc tcccggcttc acttttgcgg aaaggaagaa
    36001 cgtgcatgct agttcccatt gtcgttgagc aaaccaatcg gggcgaacgg gcctatgaca
    36061 tctactcacg cctcttgaag gacaggatca tcttccttgg tgcgccgatc gatgacgtgt
    36121 ttgccaactt ggtcatcgcc caattgctct ttctcgaagc cgaggatccg gagaaagaca
    36181 tcaatctcta cgtcaactca cccggcggga gcgtcacggc cggcctcggc atctacgaca
    36241 cgatgcagta cgtgaagccg ccgatcaata cgatctgcct gggccaggct gccagcatgg
    36301 gtgcgctgct gtt
//
LOCUS       DATIFR010000034        11040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2509137, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000034 DATIFR010000000
VERSION     DATIFR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509137"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1090)
                     /locus_tag="VLD60_01865"
     CDS             complement(410..1090)
                     /locus_tag="VLD60_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSB43723.1"
                     /translation="MSGGPLVFGLRTLRALCAVIVLSSLVACGGHAPKPTYFPGYPVG
                     YVERGMASWYGPGFHGNKTANGERYDMHLLTAAHRTLPLGSIAVVRSMSTGRKVTVRI
                     NDRGPFAKGRVLDLSLAGAKAIGMVGNGTDQIELRVVGYKGRTADMGPLRIQVGSFSE
                     QQNAVNLFERMKRAYPGGRILVVDLPEGRRYRVQVGEFKTESQAEEAAGRLESELSTQ
                     PYVVRDDG"
     gene            complement(1093..1503)
                     /locus_tag="VLD60_01870"
     CDS             complement(1093..1503)
                     /locus_tag="VLD60_01870"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43724.1"
                     /translation="MPTTTQLVISGQSRPGVMAQVASVLGSAGVNIKAFSAPEVTGTG
                     KLRLLVADLDGARAALKAAKIRFAEETALLLSLENKPGALNEVADLLTRARINIKCGY
                     CTPSREGKRAIVVLTVSNTEKALSILRDQSLDEF"
     gene            complement(1550..1915)
                     /locus_tag="VLD60_01875"
     CDS             complement(1550..1915)
                     /locus_tag="VLD60_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43725.1"
                     /translation="MSLLGLVPPRAVAAIEPRGVEEFISAEQFVVRLRLRIQEMERTL
                     SDNEQLEVAAFLPSGKAITVDTVGYENPALVILNGREQDTGKPSTLLAHQSSVQLLVS
                     VESIQPGESRRILLFTHEK"
     gene            complement(1925..2332)
                     /locus_tag="VLD60_01880"
     CDS             complement(1925..2332)
                     /locus_tag="VLD60_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43726.1"
                     /translation="MSLTLREQGIETVHDQVVSLVAQRWAKAFVCKVTIRTSLEQNPW
                     ADPHQQCDIVGWFVSSGGNAMEWMAEVETEDSLGDPQVVDKWKKTVTRGIPFYLLVPR
                     GLKEPAQKLVTDAALVCSCIYEYTFVNGVCQVL"
     gene            complement(2348..5887)
                     /locus_tag="VLD60_01885"
     CDS             complement(2348..5887)
                     /locus_tag="VLD60_01885"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSB43727.1"
                     /translation="MRIERRFTRSGQSPYEGLTFVKRSSEIRNPDGSTVFKLDQIDIP
                     DSWSQLAIDILAQKYFRKAGVPQRDEAGNPIMGQDGKPVLGGERDARQVFERLAGCWT
                     HWGKAYGYFKTIEDSAAFHDELCYMLAHQMAAPNSPQWFNTGLHYAYGLSGPAQGHYY
                     VDPKTREVVKATNAFEHPQPHACFIQSVDDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FSRLRSDGESLSGGGRSSGLMSFLRIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVVEEQKVAAMVTGSKICAQRLNAVLKACHVVDAEGTPRISIDPKQNPILREAL
                     TAARRDAVPEAYIQRMYSYAQQGFTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNE
                     FFAALEVDGDWQLKRRIDGKVSKTVKARDLWDRIAWAAWICADPGTQYDTTINEWHTC
                     PEDGRINASNPCSEYMFLDDTACNLASLNLAQFFTADGQFELEHFRHAVRIWTVVLEI
                     SVLMASFPSRAIAERSYQFRTLGLGYANLGTVLMRQGIPYDSPNALAICGALTAIMTG
                     EAYSASAEMAAELWPFPGYAKNREAMLCVIRNHRRAAYNAPKEEYEGLTIKPMGIQAE
                     YCPPDLLIAARRAWDRALELGTAYGYRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKLAGGGYFKIINQSLPPALTSLGYTESQVQDIVSYCVGRQTLQGAPFINHESLKQ
                     KGFDDAALARLESGLAQAFEIQFAFNKYVLGEDFCREKLGLTDVQLTEANFNMLKALG
                     FTQEEIAAANDYCCGTMTVEGAPHLKAEHLPIFDCANRCGRIGQRYIAVDAHIRMMAA
                     AQPFISGAISKTINMPADATLDEVKDAYLFAWKSMVKAVALYRDGSKLSQPLSASTDS
                     GKTVEASAEVLTIAEKITERILVRYLAKRRPLPARRNGYTQKAVIGGHKLYLRTGEYE
                     DGTVGEIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVK
                     LNDRIKMSTSIIDYIFRELAVTYLDRYDLAQVKEEDLRMDSVKKDEQDPECSDEEANP
                     ETLASASISTEMFPSRRGLTPRHAGNGHGNGNGSVAHKVELTRETLTLTAVQVARQKG
                     YEGDPCMECKQFTMVRNGTCLKCETCGATSGCS"
     gene            6713..7012
                     /locus_tag="VLD60_01890"
     CDS             6713..7012
                     /locus_tag="VLD60_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43728.1"
                     /translation="MFVWSKRLVAVLLGLFVLMLGVFLVHGAPDSTSGLKIHTTRWMA
                     LNYIGLIIWVFVWMIDQARMRAKNVWIWLVPFALAPLPTLLLFVLFLQRRMASTS"
     gene            complement(7007..8266)
                     /locus_tag="VLD60_01895"
     CDS             complement(7007..8266)
                     /locus_tag="VLD60_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB43729.1"
                     /translation="MATGSLKQLLGNKRIGIMLPLGFASGLPLALTAGTLQAWLAVVG
                     VDLKTIGIFTLVGLPYTLKFLWAPLMDRIVPPWLGRRRGWMLLTQIAVAIGLAFMGLT
                     GPGDHPEWLGVIALGVAFMSASLDIVYDAYRTDLLLPHERGFGAAVWVNGYRFALLLA
                     SAGALILADYIGWQGTYLLLAALMGTGVVTILLSPEPATVAAGPANLAEAVGLPLQEL
                     FARPGIYGLLSLIVLYKVGDAIAGSLQTAFFIGGLGFSASDVGYVKGIGIAATLLGAL
                     AGGLAMAKLGLVRSLLLFGALQALSNLSFMILAWIGKSYVGLAASIVVENVTGGMGTA
                     AFVALVMSLCDHRFTATQFALLSSLEALGRVFSGRPSAELVALVGWAQFFFLSFLLAL
                     PGVWLVWQYRNQLPQEAEEGNMREASL"
     gene            complement(8392..8799)
                     /locus_tag="VLD60_01900"
     CDS             complement(8392..8799)
                     /locus_tag="VLD60_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43730.1"
                     /translation="MIVRRGVTIFFLFIALLGSGSAGWSKELLPVEPDVSTRVDELYD
                     HEARLFLMLFSLNGNGKIDYVTGRLVQEYARSNFGNPVYQTEVHPLFYWWNHTMWNDP
                     EQDGVNGNERVYQENVEFDISRYKPCSFNGQPC"
     gene            8930..10264
                     /gene="mtaB"
                     /locus_tag="VLD60_01905"
     CDS             8930..10264
                     /gene="mtaB"
                     /locus_tag="VLD60_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HSB43731.1"
                     /translation="MTKRASLHTLGCRLNQAETAVLESRLRRDGYDVVEFGQPTDLLV
                     VNTCSVTDEAERTCRYVIRKTLSHSPDAFVAVTGCYAQTGVHELRTIPGIDLIVGNQF
                     KWDLPSFLPAPQELRKQPTPEVLHTKTIDREDFALPDFGEPDSTRALLKIQDGCNVMC
                     SFCLIPFARGHERSRLANDVLLEAKLLVKGGYREVVLTGVNIGQYHQDGVGLVELIKR
                     LETIEGLERIRISSIEPTTVTDALLHRMASSAKLCPYLHIPLQSGDDEILSAMHRPYS
                     VAEFSSLIQRAATTVPGLGLGTDLMVGFPGESETAFAQTVDQARQLPFSYFHVFTFSK
                     RPGTTAAKLSGEVPVHVARQRAKLLAGLSREKRLAFAEQAIGATVPVLFESGETEGFR
                     LGTTPHFLKVAVASEVDLTNQIRQVRITGASDRWAVGQLQASDKAERRIPLL"
     gene            10261..>11040
                     /locus_tag="VLD60_01910"
     CDS             10261..>11040
                     /locus_tag="VLD60_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB43732.1"
                     /translation="MNGQRHPRQVHIETFGCQMNEYDSELVRSLLKREGYVFTEDRER
                     ADIILMNTCAIRENAHTKVYTHLSDLRALKKSRPLVVGVLGCMAQNLKGELTAKEPLV
                     DVLAGPDAYRQLPMLIDNAVRSQEQGEALKGLAVDLSEYETYDDVIPERRDGVNAWIA
                     VMRGCDNFCSFCVVPYTRGRERSRDPIGIVEEARLIAAQGFKQITLLGQNVNSYRFGE
                     WDFARLLTAVADVPGIERVRFTSPHPKDFPPALLEAVATHPH"
BASE COUNT         2458 a         3219 c         3026 g         2337 t
ORIGIN      
        1 cgaagataga gggccatctt ggcccgattg ttgttgttct gggcaagagc acgcagcttg
       61 gtctcattga cggcgaagaa gccgatctcc gccgtcgaaa tcacggcaga aagaacaata
      121 aggcccaata agatcgcaat atccatggga ataggattag ggacggccgg tgaagccgat
      181 taaccagtga taaccgggaa tcagcacacc accacccaaa tgaacagagc gatggcgggt
      241 agcagatcga tctacgggaa cttgaaaggg gcttacgggg caaatcgtat tcatggtgag
      301 tagatctatc ataggaaggg gtcaggggca agcacatggt gaaaaccagt catgaaatca
      361 taggcttgca ttttatgtcc gcgagaacta tgaggtttta tggttcgcgc tatccgtcgt
      421 cacgaaccac gtacggctgg gtcgacaact ccgactccaa gcggcctgca gcctcttcgg
      481 cctgggactc ggtcttaaac tccccgacct ggacccgata gcgacgaccc tccggtaggt
      541 ctacgaccag gattcgccct cccggatagg ctctcttcat ccgttcaaag agattaacgg
      601 cattctgctg ctcggaaaag gaaccaacct ggatcctgag aggtcccata tcagcggttc
      661 tccccttgta ccctacgacg cgcagctcga tctggtccgt tccgttcccg accatcccga
      721 tggccttggc cccggcaagc gacagatcca ggactcgtcc cttcgcgaaa gggccccgat
      781 cattgattcg tacggtgacc ttccggccgg tgctcatgga acgaaccacg gcaatggatc
      841 caagcggcag tgtgcgatgg gcggctgtca gcagatgcat atcgtagcgc tcgccgtttg
      901 cggtcttgtt tccatgaaac cccggtccat accacgaggc catgccgcgt tcgacatagc
      961 caacaggata tcccgggaaa taggtcggct tgggagcgtg gccgccacag gccacgaggg
     1021 aggacagcac aatgacagcg cacaacgcgc ggagggtgcg gagcccaaag acaagaggtc
     1081 cgcctgacac ggctagaact cgtcgagtga ttgatcccgg agaatggaaa gggctttctc
     1141 ggtattcgac acggtcagga cgacaattgc ccgcttccct tcccgagagg gggtgcagta
     1201 gccgcatttg atattaatac gggcccgtgt gagaaggtcg gcgacttcgt tcaacgcgcc
     1261 tggtttattc tccaagctga gcagcaatgc cgtttcttca gcgaacctga tctttgccgc
     1321 tttcaatgcg gcgcgggcac catcaagatc cgcaaccaga agtcggagct tgcctgtccc
     1381 cgtcacttcc ggagctgaaa atgccttgat attgacgcct gccgaaccca agacggaggc
     1441 cacttgggcc atcacacctg gccggctttg tccactgatg accaattgcg tggttgtggg
     1501 catgtctacg actccgtatc ctggttatcg ggaaccagca tgactcctat catttctcgt
     1561 gggtgaacag caatatccgc cggctctctc caggttgaat cgactcgacc gagaccagga
     1621 gctgaacgga ggattgatga gccaggagcg tactcggctt tccggtatcc tgctctcggc
     1681 cgttcagaat gaccagggcg gggttttcat acccgacggt atcgacggtg attgcctttc
     1741 cggatggaag aaatgccgca acctcgagct gttcgttatc cgaaagtgtt cgctccattt
     1801 cttgaatacg aaggcgaagc cgcacgacaa actgctcggc tgaaatgaat tcttccacgc
     1861 cccgaggctc aatcgcggca actgcacgag gaggaactaa tcccaacaaa ctcatgacag
     1921 caccctacag gacctgacaa accccgttca cgaacgtgta ttcatagata cagctacaga
     1981 ccagcgcggc atcggtcacc agcttctgcg ccggttcttt caggccgcgc ggcacgagca
     2041 gataaaaggg aatgcccctc gtcacggtct ttttccattt gtcgacgacc tgtggatcgc
     2101 caagcgaatc ttcggtctcg acttccgcca tccattccat agcattgccc cctgagctca
     2161 caaaccagcc gacgatatcg cattgctgat gggggtcggc ccaggggttc tgttccaggc
     2221 tcgtgcggat ggtcactttg cagacgaagg ctttggccca acgctgagcc accaggctga
     2281 cgacctgatc gtgaaccgtc tcaatgccct gttcccgaag tgtcaagctc atcggatgtc
     2341 cccggcgcta tgaacagccg cttgtcgctc cacaagtttc acacttgagg caggtcccgt
     2401 tgcgcaccat cgtgaactgc ttgcactcca tgcacggatc cccttcgtag cctttttgac
     2461 gggccacttg cacggccgtg agggtcaacg tttcacgggt caattcgact ttgtgcgcca
     2521 cgctgccgtt cccgtttcca tgcccgtttc cggcatgacg cggggtaaga ccccgacgcg
     2581 agggaaacat ctcggtgctg atcgatgccg aggccagcgt ctccggattg gcttcctcat
     2641 cggagcattc cggatcctgc tcgtctttct tcacggaatc catccgcaga tcctcttcct
     2701 tgacctgcgc cagatcgtac cgatccagat acgtcacggc gagttcgcgg aagatatagt
     2761 cgatgatcga cgtcgacatc ttgatgcgat cgttcagttt gaccggtccg ttcggctcaa
     2821 aacgcgtaaa gacgaacgct tcgacgaatt cctccaaggg aacgccgtgc tgcagaccga
     2881 gagaaatcgc gatggcaaaa caattcatca ggctgcggaa ggccgcgccc tccttatgca
     2941 tgtcgaggaa aatttcgccg accgtgccgt cttcgtattc accggtccga agatacagct
     3001 tatgcccccc gatcacggct ttctgcgtgt acccgttccg tctggcagga agtggccgcc
     3061 gtttggccag gtatcgaaca aggatccgtt cagtaatttt ttccgctatg gtgaggactt
     3121 ccgcggaagc ctcgactgtc tttccgctgt ccgtcgacgc ggacaacggc tggctcaact
     3181 tggaaccgtc cctatagagc gccacggcct tgaccatact cttccaggca aagagatagg
     3241 catccttgac ctcgtccagc gtggcgtcgg ccggcatatt gatcgtcttg ctgattgcac
     3301 cgctgataaa cggctgtgcc gccgccatca tgcgaatgtg cgcatcgacc gcgatgtatc
     3361 gctgaccgat gcgaccgcac cggttggcgc aatcgaaaat cggcagatgt tcagccttga
     3421 gatgcggcgc gccttcgacc gtcatggtgc cgcagcaata atcgtttgcc gccgcgattt
     3481 cctcctgggt aaatccaagg gccttcagca tgttgaaatt cgcctcggtc aactggacgt
     3541 cggtcagtcc gagtttctcg cggcagaaat cttcgccgag tacgtattta ttgaaggcga
     3601 actggatttc aaaggcctgc gccaatccgc tctccaggcg cgccagcgcc gcatcgtcga
     3661 atcctttttg tttgagcgat tcatgattga tgaacggtgc gccctgcaag gtctgccggc
     3721 cgacacaata gctgacgatg tcctgcacct gggattccgt ataacccaaa ctcgtcagag
     3781 cgggcggcag gctctggttg atgatcttga agtagccgcc gccggccagc tttttgaatt
     3841 tcaccagggc aaagtccggc tcgattcccg tcgtgtcgca atccatgacc agaccgatcg
     3901 tgccggtcgg cgcaatgacg gtcacctgcg cgttgcgata tccataggcc gttcccaatt
     3961 cgagcgcgcg atcccatgca cgccgtgcgg caatcagcaa gtcgggagga caatattccg
     4021 cctgaatgcc catcggcttg atcgtcaatc cttcatactc ttcctttggc gcgttgtacg
     4081 ccgcacgacg atggttgcgg atgacgcata acatcgcttc acgatttttg gcatagcccg
     4141 ggaatggcca tagttctgca gccatttccg ccgatgcgct gtatgcctca ccggtcatga
     4201 tggcggttaa cgcgccgcaa atcgccagtg cattgggaga gtcgtaggga atcccctgcc
     4261 gcatgagcac cgtaccgaga ttcgcatatc cgaggcccag ggtccggaac tggtagcttc
     4321 tctcggcaat cgcccggctg gggaacgacg ccatgagcac gctgatttcc aggacgacgg
     4381 tccagatgcg cacggcatga cggaaatgct ccagttcgaa ctggccgtcg gctgtgaaga
     4441 actgggcgag gttcaacgag gccaggttgc aggctgtgtc gtcgaggaac atatattcgg
     4501 agcagggatt cgacgcattg atacgtccat cctccgggca ggtgtgccac tcgttgatgg
     4561 tcgtatcgta ttgcgtgccc gggtcggcgc aaatccatgc ggcccaggcg atgcggtccc
     4621 acaagtcgcg cgctttgacg gttttagaaa ccttgccgtc gatccgacgt ttcagttgcc
     4681 aatcgccgtc aacctccaaa gccgcaaaga actcgtttgg aatacggaca ctgttgttgg
     4741 agttttgccc ggataccgtt tgatacgcct tgccgtccca attcgtatcg tattcgtgaa
     4801 atacaaaatg tgtgaaccct tgctgcgcgt aggaatacat ccgctgaatg taggcttcgg
     4861 gcacggcatc gcgccgggcc gccgtgagcg cttccctcag aatcgggttc tgcttcgggt
     4921 cgatcgagat gcgcggtgtg ccttctgcgt cgacgacatg acaagccttg agcacggcat
     4981 tcaggcgttg ggcgcagatt ttcgagccgg tcaccatcgc cgcgactttc tgttcttcga
     5041 ccaccttcca atcgataaat tcctcgatat cgggatggtc caaatccaag cagaccatct
     5101 tcgccgcccg cctcgtggtg ccgccggact tgatcgcgcc agccgcccgg tcgccaatcc
     5161 gcaggaacga catcaatcct gacgaacgcc cgccgcctga aagcgactca ccgtcgctgc
     5221 gcaatctcga aaaattcgtc ccggtgccgg agccatactt gaacaggcgg gcttcccgca
     5281 cccataaatc catgatgccg ttctcgttga cgagatcgtc atcgacggat tggatgaaac
     5341 aggcgtgcgg ctgcggatgt tcgaatgcgt tggtcgcttt cacgacctcg cgggtcttcg
     5401 gatcgacgta gtaatggcct tgtgccggac cggacaaccc atacgcatag tgcagtcccg
     5461 tattgaacca ctggggcgag ttgggtgcgg ccatctggtg ggccagcatg taacacagct
     5521 cgtcatggaa ggcggcactg tcttcaatgg tcttgaaata gccgtaggct tttccccagt
     5581 gcgtccagca accggccaat cgttcgaaga cctgccgggc atctcgctct ccccctaaaa
     5641 ccggcttgcc gtcttgtccc atgatcggat tgccggcctc atctcgttgg ggcactccgg
     5701 cctttcgaaa gtatttctgc gccagaatgt cgatggcgag ctgggaccat gagtcgggaa
     5761 tatcgatttg gtccaacttg aagacggtcg aaccatcggg gttacggatc tccgacgagc
     5821 gtttcacgaa cgtcaagcct tcatagggac tttgtccgga ccgtgtaaat cgtcgttcta
     5881 ttctcacgat ctctcctccc cccaagggta atgtggtcgc tctaggtggc tttggactga
     5941 gtcggaaaac cggaggcgct gagacgggtc atgctacctc agattatcgt gtcaaaaact
     6001 gccggctgca tgttctgcct acggggatcc agcagcggaa tgactacata tagccattcc
     6061 gaataattgt caataccaaa tattgtggtt cggttgggga tagtgaggaa agcctgtgga
     6121 taactgctgc ttcgtaccag tggctgattt cggacgattt acggtgtttt atccacagat
     6181 tttgagggtg aaaacgagcc tgtccgcaca accaagcccc tgccgaaacg tgaaaactag
     6241 aaggccatcc tcccgaggcg aagtatggcc tcgtcgttac cctttagagg aatgaaccgg
     6301 ccaccaaccc caacaacaag aatctgtgag tcgaataatc tactctcata cgagctcgca
     6361 agtccccaat tatggcgaac ggtgcgagga cgaacgaagc tcgtgaccag ctcgtgacta
     6421 tgtgaacgat agattgatct aaccatggcg ccttcgcggc tacctggcgg ctgatcgctt
     6481 cgatccaatg cttttcccaa ttcggcttca acaaacctta agtaatcatc catagtggga
     6541 ttcgtgagcg cgaggccgat gcacaagctc aagaacgcca ccaacaggat caggcggagt
     6601 acagtcacgc ggatcaccct ttgacaaaat ccagtatcat cattagccta gcgtatcctg
     6661 cgtcgtcaaa tcgaacgggt gtagtcgtgc aaccaaccgc gctggagggc atatgttcgt
     6721 ttggagcaaa cgtcttgtgg cagtactctt gggactgttc gtgctcatgc tgggggtctt
     6781 tctggtgcac ggcgctcccg acagtaccag cgggctgaaa attcatacga ctaggtggat
     6841 ggccctcaac tacatcggct tgatcatctg ggtctttgtt tggatgatcg accaggcccg
     6901 catgcgggcg aagaatgtat ggatctggct cgtccccttc gcccttgccc cattgccgac
     6961 gctcctcttg tttgtattat ttcttcagcg tcgtatggca tcgacttcat aggcttgctt
     7021 ctcgcatgtt cccctcctct gcctcctgag gaagttggtt tcgatactgc cagactaacc
     7081 agacaccggg cagagccagc agaaaggaga ggaagaaaaa ctgcgcccat cctaccagtg
     7141 ccaccaattc ggccgaggga cgcccagaga aaactctccc taacgcttcc agtgaagata
     7201 acaacgcaaa ttgcgtcgcc gtaaaacgat gatcgcagag cgacatcacc aatgcgacga
     7261 aggccgcggt acccatcccg cccgtgacat tctccacgac gatcgaggca gcaagaccga
     7321 cataactctt gccgatccac gccaggatca tgaacgaaag attggagagg gcctggaggg
     7381 caccgaacag caacaacgag cgcacgaggc cgagtttcgc catcgcaagc ccaccggcca
     7441 atgcacccag cagcgtggcc gcgattccga ttcctttgac gtagcccacg tcgcttgcag
     7501 aaaaccctaa acctccgatg aagaaggcgg tctgcaacga gcctgcgatg gcatcaccga
     7561 ccttataaag cacgatgagg ctcagcaatc cataaattcc gggacgggca aacaattcct
     7621 gaaggggaag tcccacggct tccgccagat tcgccggccc tgcggcgacc gtcgccggtt
     7681 caggactgag cagaattgtc acgactccgg ttcccatcag cgctgctaga agcagatatg
     7741 tgccttgcca tccgatatag tcggccaaaa tcagggcgcc ggcactggct agcaacagtg
     7801 caaagcgata gccgttgacc cagacagcgg caccgaatcc ccgctcatga ggcaggagca
     7861 gatccgtccg ataggcgtca tacacgatgt cgagtgaggc ggacataaat gcgacgccaa
     7921 gcgcgataac tcccaaccac tccggatgat cgcccggacc ggtgagtccc atgaatgcca
     7981 ggccgatggc cacggcgatt tgcgtcagca acatccagcc acggcgccga cccaaccaag
     8041 gaggcacgat ccgatccatc agcggcgccc acagaaactt caacgtgtaa ggcagcccaa
     8101 ccagcgtgaa gatgccgatc gtcttgaggt caacaccgac cacggccagc caggcctgta
     8161 acgtaccggc cgtcagagcc agcggcagac ctgaagcgaa gcccagcggc aacatgatgc
     8221 cgatgcgctt gttgccgaga agttgtttaa gagaacccgt agccattgga cgtgagtgac
     8281 ttgagctgtg cggctagcat aacaccggcc ggagcacagc ctcaggagat tcacgaaatg
     8341 cgtctgagcg gcaatgaaac ggctggacgg tccgaaggag agactggaga cctagcaagg
     8401 ttggccgtta aaggagcagg gcttatagcg ggaaatgtcg aactctacgt tctcttggta
     8461 cacacgttca tttccgttga ctccgtcctg ctccggatcg ttccacatgg tgtggttcca
     8521 ccaatagaaa agaggatgga cctcggtctg gtacacagga ttgccgaagt tgctccgcgc
     8581 atattcctgc accaatcgcc cggtgacata gtcgatcttt ccgttgccgt tcaaggaaaa
     8641 gagcatgagg aacaaccgag cttcgtggtc atacagctca tcgactcgcg tgctcacatc
     8701 cggttcgacg ggaagcagct ctttcgacca gccggcactc ccggaaccta gcagagcaat
     8761 gaagaggaaa aagatggtga cgccccggcg aactatcacg ataggcttcc cctcgtgcgc
     8821 gcaatgatgc ggtccatgct accacgctat tggaaggcct tcaaccggag gcctggaagg
     8881 cagaggcacc ttgctcggcg atcgagcatc tctctataat gtgcagctta tgacgaaacg
     8941 cgcgtcactt cacacgttgg gctgtcgcct caatcaggcg gaaacggccg tgctcgagtc
     9001 tcgactgaga cgagacggct acgatgtcgt cgagttcggt cagcctacag atctgctcgt
     9061 cgtcaacacc tgctctgtca cggatgaggc agagcgaacc tgccgctatg tcattcgtaa
     9121 aacgttgagc cattcccccg acgccttcgt ggcagttacc ggctgttacg cacaaaccgg
     9181 cgtgcatgaa ttgcggacga ttcccgggat tgatctcatt gtcggtaacc agttcaagtg
     9241 ggacttgccc tcgttcttgc cggcccccca ggaattgaga aagcagccga cccccgaagt
     9301 gctccatacg aagacgatcg atcgcgaaga tttcgcactt cccgacttcg gcgaacccga
     9361 ttcgacgaga gcattgctga agattcagga cggctgcaac gtgatgtgca gcttttgtct
     9421 cattcccttc gcgcggggcc acgaacgcag ccgtttggcg aacgatgtgc tgctcgaagc
     9481 aaagctactc gtcaagggtg gctaccggga agtcgtcctg acaggcgtca atatcgggca
     9541 atatcatcaa gatggggtgg gcctcgtcga actcatcaag cgactggaga ccatcgaagg
     9601 tcttgaacga attaggattt cgtccatcga accgacaacg gtcacggacg ctctcctcca
     9661 tcgcatggca tcatccgcga aactctgccc ctatctccac atcccgctcc aaagcggaga
     9721 tgatgaaatc ctttcagcca tgcatcgccc ctacagtgtc gcggaattta gctcgctcat
     9781 ccaacgagcg gccacgacgg ttcccggact tggtttagga accgacttga tggtcggatt
     9841 tcccggtgaa tccgaaacgg cgtttgcaca gacggtggac caagcccgac aattgccctt
     9901 ctcatacttc cacgtcttca cattctccaa gcgaccaggg accaccgccg caaagctttc
     9961 cggagaagtt ccggtccatg tcgctcgaca gcgagcaaaa ctcttggctg gtctctctcg
    10021 cgaaaagagg ctggcctttg ctgagcaggc gatcggagcc actgtcccgg tcctcttcga
    10081 gtcgggtgaa acagaaggat tcaggctggg aaccacgccg cacttcttga aggttgcagt
    10141 ggcctcagaa gtcgacctga ccaaccaaat ccgacaagtg cgcattaccg gagcatcgga
    10201 tcgttgggcc gtggggcagt tgcaggcgtc cgataaggcg gaacggagaa taccccttct
    10261 atgaacggac aacgccatcc ccggcaggtt catattgaga cgttcggatg ccagatgaac
    10321 gaatacgact cggaacttgt tcgttcgcta ctgaagcgag agggctacgt attcacggaa
    10381 gaccgagaac gcgccgatat cattctcatg aatacctgtg ccatccgtga gaatgcacac
    10441 acgaaagtct atacccacct ctcggatctc agggcgctta agaaaagtcg ccccttggtt
    10501 gtgggggtgc tcggatgtat ggcgcaaaat ctcaaaggag aactgacagc aaaagagccg
    10561 ctagtggatg tgctggccgg tccggatgcc taccgtcaac tgcctatgtt gatcgacaat
    10621 gcggttcgat cccaggagca gggcgaggca ttgaaaggtc tggccgttga cttatccgag
    10681 tacgaaacct acgacgacgt gataccggaa cgccgtgacg gtgtgaacgc ctggatcgcc
    10741 gtcatgcgcg gatgcgacaa tttttgcagt ttttgtgtgg tgccttatac gagaggccga
    10801 gagcgctcga gagacccgat cggcatcgtg gaagaggcgc gcctgattgc agctcaggga
    10861 ttcaagcaaa tcacgctcct cggccagaac gtcaactctt atcgtttcgg tgaatgggac
    10921 tttgcccgat tgctcacagc ggtcgccgat gtgccgggaa tcgaacgcgt gcgctttacc
    10981 tctccccacc ccaaagattt ccctcctgcc ttgctggagg ctgtggccac acacccacac
//
LOCUS       DATIFR010000035        13031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2126153, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000035 DATIFR010000000
VERSION     DATIFR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126153"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VLD60_01915"
     CDS             <1..340
                     /locus_tag="VLD60_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43733.1"
                     /translation="VLLDSTPPGISSSHVAAVIETFPGVKNVHDLHIWAVEPRLIMLT
                     CHVLVDGEDNALTNVLLQSIRDRIASDFGIKHMTIQMETSCSHPEAVHCDLRKLAEQH
                     RDGESLHVHR"
     gene            352..1320
                     /locus_tag="VLD60_01920"
     CDS             352..1320
                     /locus_tag="VLD60_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB43734.1"
                     /translation="MSQPPVPFYVLCGALGAGKTTLLMRLLEHWKGHGIRAGVLMNEA
                     GEVSIDGPRVGTIAEQVMNLAGGCVCCDTKEDLSWGIAQLVKDYGSELIVLECSGLAD
                     PTEVVDAVTDLYTARLAKLERIIALLHPISAEQSASSEYVTTQAIRCADELILNKRDL
                     YVPGHWEQFKDAIIAKNAMARVWETTHARIDPTALLAPHPSAKAQIPMNVVFGKPQLS
                     PATARAAFHPIATTVRLPGPLNRQRFLAWSKTLPKEMERAKGYFRFAQEPELQEFQYA
                     PPDQATITPVMLLDEPDQAIVLIGRGYDVARWQENLLCCVETRKTT"
     gene            complement(1317..1889)
                     /locus_tag="VLD60_01925"
     CDS             complement(1317..1889)
                     /locus_tag="VLD60_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSB43735.1"
                     /translation="MNLDESLFRAINGLAGQSTSLDWLALTLSSPDTLWTPGILLSCY
                     WLWLSWREAVIAAPVLGGLIGLLDFIGAQLKHVVARSRPCMVLSDIHQLEACGKVFGF
                     PSNHAINTATAAAFLQVLYPRSGWVSWPLVFLVGVARVFIGAHYATDVIGGWVLGGVC
                     GAGAAWLLLQWPRFREQSIISAPPVERASD"
     gene            complement(1917..2378)
                     /locus_tag="VLD60_01930"
     CDS             complement(1917..2378)
                     /locus_tag="VLD60_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43736.1"
                     /translation="MSVISDSMINATRRIVFCCALWVCLLPVAAYPVVGDEATHETDH
                     QEDVLELPEVHVHGLPLNKDQQLGPVVKLTPWPGIPPSLDGKELDDWMKARLLVSKDA
                     KVTVVILEPCKHRELTVAGMAALGKWTFEPQMKGDEPIDGELTVRIHFHTR"
     gene            complement(2394..4733)
                     /locus_tag="VLD60_01935"
     CDS             complement(2394..4733)
                     /locus_tag="VLD60_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB43737.1"
                     /translation="MTSLLSAAEGDALTTIQGTVQNQDLRRVAQAVVEVKNQEGVTIA
                     TGVSNDAGEFSLSVPAGGTYSVSAVQETYRSEYVVLVLGADQPKPVALTLSKTKEIAL
                     EVVAPLPPIQYKASSETYSVSRKDIETLPKGNNNDVYEVLLTIPSVAYGALKQTHIRQ
                     DHANAQFRIDGIPIPDTVSGAFADIVPPRAWERADMILGGMEAQYGNRTVLVLDITSK
                     SGTRPGFGSVQMFGGSNETVNPSFEYGGAIGEKVRFYVLNSFVSTNRGIEPPTLGKTN
                     DHNQSEKNNTYLRGDYQHDNRNSISWVFLNSVAKYQIPTIPNLEANPDVLTLLPGFTP
                     NASQSVNQFQKENNQYSHLVLRHDVNAANFFNVGLYFRRGQADFNTDPLNALAFESDV
                     NSAQTSNQRRMAYSGGVRVDHTWVPNSQHLFKTGMQIDYSRANNTAQIFAFDTSGGTP
                     VGPVISREASNQNIQWREEFWVQDQWSPNSQWTFNVGARLDVIQGFYNEGQVSPRVGI
                     TYKYNQANVFHAYYGRLFTPPNIEQVAFVRLNVQNTTAQPDDPTGFSPRAERSHYFEV
                     GSYHAISRYATLELTGYYKLDHFMSDAGQFGSTPLLNSFAFERGWKRGIDGALKVQIT
                     DDLTGRGNVAWGQCKGYGLQSGQYLLDQGEINDINSNGGVFCDHSQFMTSSAVLSYRL
                     LERTHVTGQMLYGSGLRTAQEGAKTNSSHENSYTVYNASITHTIPLPWHDQKMLLGFD
                     ILNLLDQQYFYNRGEGSIGLGIAHAGMPRSYFFRAQWFF"
     gene            complement(4909..5229)
                     /locus_tag="VLD60_01940"
     CDS             complement(4909..5229)
                     /locus_tag="VLD60_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43738.1"
                     /translation="MNSSNLSNRLRSGLSLGLILLLLAIAPFAVAQDVHHELAAADTD
                     GHEHSDTDLCQWVQHHTSGSIDLGGPIFSAWDTVKQFDVPAPTILLSVEFASVGPSRA
                     PPRI"
     gene            5393..6451
                     /locus_tag="VLD60_01945"
     CDS             5393..6451
                     /locus_tag="VLD60_01945"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSB43739.1"
                     /translation="MNRPLDNQHVIEIKALPSPRAIKTKLPITDDAAALVVETRDAIR
                     RILHGQDRERLLVIVGPCSIHDPESAYEYAERLNSVADALRDRLLIVMRTYFEKPRTT
                     IGWKGLINDPHLDGSCDIATGMELARTILLKINQRGVPCASELLDPISPQYIADLISW
                     TAIGARTTESQTHREMASGVSMPVGFKNGTEGSLQVAINAMTSARTPHHFVGINADGQ
                     TSIIKTMGNPDRHIVLRGGGGKTNYDAEHVARAEAALAGEGIARPIMIDCSHDNSSKD
                     HKRQGLVARDVLRQFREGRQSIMGLMLESNLNPGKQTWKQGIPLAYGVSITDACLGWD
                     ETASLLNELADSIVTKPA"
     gene            6496..7275
                     /locus_tag="VLD60_01950"
     CDS             6496..7275
                     /locus_tag="VLD60_01950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSB43740.1"
                     /translation="MLIDTHTHLDDARYNDDRDAMIARAREAGVGAFVTIGCDLSTSR
                     AAVALADQYSFVYASIGVHPHEVKHIGDGWYDDFRRLARNKNVVAYGEIGLDYHYNHS
                     SPKDQRDRFREQIQLARELKLPVIIHTREAQEDTIAILKEEKASDVGGVFHCFSGDAW
                     LAKDALDLGFYLSFAGILTFQNATMLREIAKNTPLDRVLIETDCPYLTPVPHRGKRNE
                     PAYVSYVAQQLAEIHSGTNSLSAARIGELTSANAKRLFKIA"
     gene            complement(7262..7786)
                     /locus_tag="VLD60_01955"
     CDS             complement(7262..7786)
                     /locus_tag="VLD60_01955"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSB43741.1"
                     /translation="MAMHVIIDGYNLLARIGGMGVLHSEMAREALLRDLAAYRQRKGH
                     AVTVVFDGWQHGQPIEGREHRAGVQVIYSKRGERADQVIQRLAREYGSDCAIVSSDHE
                     IVATAKAHGAFIMGAQEFSDKLRALSQFAGVLAHKELDTEEDSRTRRDQEKKGNPRKL
                     PKAQRQRSKQLRRF"
     gene            7916..9196
                     /locus_tag="VLD60_01960"
     CDS             7916..9196
                     /locus_tag="VLD60_01960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSB43742.1"
                     /translation="MPLAYWRTACLILLTGLSGLQGTFPVVAGLDQVLAATQETRREQ
                     PRHSKRSRTEQVSLAPVRMSPAMIENLRTAVESGSTRLVLDLDRKTRARKYIPAQADG
                     VILEIPHAILSKSAQTKIANGTLAKPFVIRQTSADTVAVSLPNGTFHTYKLFSLTNPP
                     RLVIDVAPSSPQDFSRPLEPTEPSRLPDPPPQPNPPRAKSFTTIVIDPGHGGKDPGAH
                     GRRGTEEKDITLKVGLKLRDLLSRQPGIRVLMTRERDVFVELEDRAKFANGNDADLFV
                     SIHVNSHPQRSVRGIEIYHFGEAKDQRALEVAARENGTPLSSTGVGWEYLVADLLTAK
                     KIETSLELAWNTKEAMITHLNGHYALVDHGVKTAPFYVLRYTSMPSILAEIAYISNSA
                     EEDLLRTSAFTTRVAESLLEGVQAFLVASKQPAR"
     gene            9225..9965
                     /gene="truA"
                     /locus_tag="VLD60_01965"
     CDS             9225..9965
                     /gene="truA"
                     /locus_tag="VLD60_01965"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSB43743.1"
                     /translation="MPTVKLIVEYDGSRYAGWQRQPDQPTIQEALETAIYQLTQHKVS
                     VIGAGRTDAGVHALGQVVSFRIERDWTAREWTRGFNARLPDDIAVRSASLMPDEFHAR
                     YGACGKLYEYRILNRPERPAIDRAYVWHVYKPLNDEAMRQAASHLIGSHDFTSFEGTL
                     TDNEDPSCNLQQLSLTRQGDHLVIQAYADRFLKHMVRAIVGTLAEVGHSKRTPQSMTE
                     VLVAKDRTVAGRTAPPQGLFLVRVDYNH"
     gene            10053..10364
                     /locus_tag="VLD60_01970"
     CDS             10053..10364
                     /locus_tag="VLD60_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HSB43744.1"
                     /translation="MKIASLLVLLLFTSGIALAPMASEAADQQNKMQACNEQANAKGF
                     GEGKGDERKAFMKECLSAKPAKSGKTAQQEKMKACNKEAGEKKLAGDERKKFMSTCLS
                     N"
     gene            complement(10521..11171)
                     /gene="cysE"
                     /locus_tag="VLD60_01975"
     CDS             complement(10521..11171)
                     /gene="cysE"
                     /locus_tag="VLD60_01975"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HSB43745.1"
                     /translation="MLKAIRQDLKAVFDRDPAATSRLEVIFTYAGFHALLAYRISHWL
                     KIHRVPFIPRLISQFARWLTGIEIHPSAKIGTGFFIDHGMGVVIGETAEIGDYVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGITIGDNVKIGANSVVLKNVSANSTV
                     IGVPARVIKTQGERLPDATMDHINLPDPVSDRLLALEQELIELRKKLENQDTPPSR"
     gene            complement(11265..11633)
                     /locus_tag="VLD60_01980"
     CDS             complement(11265..11633)
                     /locus_tag="VLD60_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSB43746.1"
                     /translation="MAKIERFEDIKAWVMAKELVAAIYGLSGNGKFAQDFGLRDQIRK
                     ASVSVMSNIAEGFERGSQKEFSRFLRIAKGSSGEVRSQLYIALELGYLDRADFEEVKA
                     KAENLSKALSGFIGYLARGN"
     gene            complement(11693..12178)
                     /gene="ispF"
                     /locus_tag="VLD60_01985"
     CDS             complement(11693..12178)
                     /gene="ispF"
                     /locus_tag="VLD60_01985"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HSB43747.1"
                     /translation="MATGARIGYGYDVHPLGPGRKLILGGIEIPHNKGLLGHSDSDVL
                     VHAVCDALLGAIGEGDLGRHYPSSDPRYKGISSLKLLEDVVSKLKVKGYRIGNIDTVI
                     VAQAPRLGPHLVAMQKKMAEAAGIDADLVNVKVKSGEGLDAVGREEGMTAHAVCLIEP
                     L"
     gene            complement(12181..12894)
                     /gene="ispD"
                     /locus_tag="VLD60_01990"
     CDS             complement(12181..12894)
                     /gene="ispD"
                     /locus_tag="VLD60_01990"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSB43748.1"
                     /translation="MSRKTVALVPAAGRGLRMGGAVPKQFLSIGGLPLIVHSLRTLQA
                     SSVIDAIVLAVPQADLAYCQSDIVDAHRFTKVIKVVAGGQERQDSVRNALAVLDDQVE
                     IVVVHDAVRPFLTERMLEDVVAAARATGAAIIALPMRDTVKYVGADHLIERTVDRRPL
                     WLAQTPQAFRRDWLQEAHRRAQIEAVPATDDASLLEWMGRSVTVVEGSGENIKVTRPE
                     DMIIGEAILASRQSVAGKG"
BASE COUNT         3017 a         3884 c         3408 g         2722 t
ORIGIN      
        1 tgtgctgtta gactcaacgc cgcccggcat cagttcctcc catgtcgcag ccgtgatcga
       61 gacgtttccg ggggtcaaga atgtgcacga tctccatatc tgggcggtcg agccacgcct
      121 gatcatgctg acctgccatg ttctcgtcga tggagaagac aacgcgttga cgaacgtgct
      181 gctgcagtcg atccgtgacc gcatcgcctc cgatttcggg atcaagcaca tgaccatcca
      241 gatggaaacg tcctgcagtc accccgaggc ggtgcattgc gatctcagaa agctcgccga
      301 gcagcatcgg gacggcgaat cacttcatgt ccatcgatag tcgctgacgt catgtcgcaa
      361 ccccccgtcc ctttttatgt cctctgcggt gctctcggag cggggaaaac tacgctcttg
      421 atgagactcc ttgagcattg gaagggtcac ggcattcgag caggagtcct gatgaacgaa
      481 gcgggcgaag tcagtatcga cggcccccgt gtggggacca ttgccgagca ggtcatgaac
      541 cttgccggag gatgcgtgtg ctgcgatacg aaagaagacc tctcgtgggg gattgcacag
      601 ctggtcaaag actatggatc ggaactgatc gtccttgaat gctcaggctt agccgacccg
      661 accgaagtcg tcgacgccgt cacggacctc tatacggcca gactcgcgaa gctcgaacgt
      721 atcatcgccc tgctccatcc gatctcagcc gagcaaagcg cttcgagcga atatgttacc
      781 acccaagcta ttcgctgtgc cgacgaactc atcttgaata agcgggatct ctatgtgcca
      841 ggtcattggg agcagttcaa ggatgcgatc attgcaaaga acgctatggc tcgtgtatgg
      901 gaaacgaccc atgctcgaat cgatccaacg gctctgctgg cgcctcatcc atcagctaaa
      961 gcacagattc cgatgaatgt ggtgtttggc aagccacagc tgtcgcccgc cactgcccgc
     1021 gccgcattcc acccgattgc cacgacagtc cgactaccag gaccgttgaa ccgtcaacga
     1081 ttcctcgcat ggagcaaaac gctgcccaaa gaaatggaac gtgcgaaagg ttacttccgt
     1141 tttgctcagg agccggagct gcaggagttt cagtatgccc cgccggatca agccacgatc
     1201 acgccagtga tgctgctgga cgagccggat caggcgatcg tactgatcgg acgaggatac
     1261 gacgtcgcac gttggcaaga gaatctattg tgttgcgttg agacaagaaa gacaacttaa
     1321 tctgaggctc gctcgacagg tggcgcggat attattgatt gctcacgaaa ccgtggccac
     1381 tgcagcaaaa gccaggccgc gccagcgcca caaactcccc ccaacaccca cccaccgatc
     1441 acatccgttg cgtagtgggc gccgataaac acgcgcgcga cgcccacaag aaacaccagc
     1501 ggccaactga cccagccaga acgaggatac aacacctgca ggaaggcggc cgcggtggcc
     1561 gtgttgatgg catggttcga ggggaatccg aaaacctttc cacacgcctc aagctgatgg
     1621 atatctgaca acaccatgca agggcgtgac cttgcgacaa catgttttag ctgtgctccg
     1681 atgaaatcga ggagcccaat caatccgccg agcaccggcg cggcgatcac agcttcacgc
     1741 cagctaagcc agagccaata acagctcagc agaattcctg gtgtccagag tgtatcgggc
     1801 gaggacaagg tcaatgcgag ccaatcgagg cttgtcgatt gcccggcaag cccgttgatg
     1861 gcgcgaaaga gagattcatc caaattcacg cggccatcat agcatgactg gaaatgttat
     1921 cgggtgtgga agtgaatgcg aaccgtcagc tcgccgtcga tcggctcatc acctttcatc
     1981 tgaggctcga aggtccattt gcccagggcg gccattccgg caacggtcaa ttctcgatgc
     2041 ttacagggct caaggatcac aacagtcact tttgcatcct tcgataccag cagacgggcc
     2101 ttcatccagt catcaagttc cttcccatcg agcgagggag gaatgccggg ccagggagtc
     2161 aatttgacga cgggcccaag ctgctgatcc ttattgagcg gaagcccatg cacatggacc
     2221 tccggcaact ccaacacgtc ttcctgatgg tccgtctcat gcgtcgcttc atcgccgacc
     2281 acagggtacg cagcaaccgg caggagacac acccagagcg cacagcaaaa cacaatccgc
     2341 ctggttgcgt tgatcatgct atcgcttatc acgctcatgt tgaacaccat atatcagaag
     2401 aaccattggg cacgaaagaa atacgacctc ggcatccccg catgggcaat cccgagtccg
     2461 atactgcctt ctccccggtt atagaaatat tgttgatcta ataaattgag gatatcaaaa
     2521 cccagcagca tcttttgatc gtgccacggc aatggaatcg tatgcgtaat cgacgcattg
     2581 taaacggtat aggaattctc gtgggaggaa ttcgtcttgg ctccctcctg tgccgtacgc
     2641 aatcctgaac catagagcat ctggccggta acatgagttc gttccaacag tcgataggat
     2701 agcaccgctg aactcgtcat gaactgcgag tgatcgcaga atactccgcc gttagaattg
     2761 atatcgttga tctccccctg atcaagcaga tattgtccgg actgcagccc gtaacctttg
     2821 cactgccccc acgccacatt gccccgtccg gtgagatcat cggtgatttg aaccttcaac
     2881 gcgccgtcga tacctcgctt ccaccctcgc tcgaaggcaa aggaattcag caacggtgtc
     2941 gacccgaact ggcccgcatc cgacatgaaa tgatcgagct tgtagtagcc ggtcagttcc
     3001 aacgtggcgt atcggctgat cgcgtgatag ctccctacct cgaagtaatg tgaacgttct
     3061 gcgcgcggac tgaaaccggt tggatcgtcc ggctgcgcgg tagtgttctg cacgtttaac
     3121 ctgacgaacg ccacctgctc gatgtttggc ggcgtgaaga gacgaccata atacgcatgg
     3181 aagacgttgg cctgattata tttataggtg ataccgacac gtgggctgac ttgcccctcg
     3241 ttgtaaaacc cttgaatcac gtcgaggcgg gccccgacat tgaaggtcca ttgcgagttg
     3301 gggctccatt gatcctgcac ccaaaactcc tcccgccact gaatgttctg gttcgacgct
     3361 tcgcgcgaaa tgaccggtcc caccggtgtg ccgccgctcg tatcgaatgc aaagatctgc
     3421 gccgtattgt tggcccggct gtaatcgatc tgcatgccgg tcttaaatag gtgctggctg
     3481 ttaggaaccc aggtgtggtc aacccgcaca ccaccgctgt aggccatacg tctctggttg
     3541 gaggtctgcg cgctgttcac atcgctttca aaggccaagg cgttcagcgg atcggtgttg
     3601 aaatcggcct gccctctccg aaaatagagt ccgacgttga agaaattcgc ggcattcaca
     3661 tcgtgccgca agaccagatg gctgtactgg ttgttctcct tctgaaactg attgacggat
     3721 tgtgaggcat tgggcgtgaa ccccggcaat aacgtcagca catcggggtt ggcttcaagg
     3781 ttggggatcg tcggaatctg atacttggcc acagaattga gaaaaaccca gctgatgctg
     3841 tttcgattat catgctgata gtcgccgcga aggtacgtat tgttcttctc gctctggttg
     3901 tggtcgttcg tcttcccgag cgtcggaggc tcaatccccc ggttcgttga gacgaagctg
     3961 ttcaagacgt agaatcgaac cttctctccg atcgctcctc catattcaaa cgatggattc
     4021 accgtttcgt tcgatccgcc gaacatttgg acggacccaa atcccggcct tgttccgctc
     4081 ttgctcgtaa tatccaggac gagaaccgtc ctgttgccgt actgcgcttc catcccgcca
     4141 agaatcatat cggcccgctc ccaagcccgc ggaggaacaa tatcggcaaa ggccccgctc
     4201 acggtgtccg gaattggaat cccgtcgata cggaattgcg cattggcatg gtcctgccgg
     4261 atatgggtct gcttcagtgc tccatacgct acgctgggaa tcgtcagcag cacttcatag
     4321 acatcgttgt tattcccttt cggcaacgtt tcaatgtctt tgcggctgac cgaataggtt
     4381 tcgctcgagg ctttatattg aatgggcggt aacggcgcga ctacttccag ggcgatctct
     4441 ttggtcttgg ataaggtcaa cgccacgggc ttgggttgat ccgcccctaa tacgagcacc
     4501 acgtactcgc ttcgataggt ctcctgcacc gcactgacgg agtaggtccc accggcagga
     4561 acagaaaggg agaactctcc ggcatcgttt gaaacaccgg tcgctatggt cacaccctcc
     4621 tgattcttca cctcgaccac cgcctgggcc acccgcctca agtcctggtt ctggacggtt
     4681 ccttgaatcg tcgtcagcgc atcgccttcg gcggcagaga gcaaggaagt catcccccac
     4741 ccacctgaga ccaccagtat gaggaacaag atcaatccac tacccatcgg cacagaccgt
     4801 cgcatcggaa cctctagaac cattcgctat agaaaacaag aaatggtgcc gtggctgccg
     4861 cctcatgaac agacggagcc aacccataca gcacgattcg ttaggagctt agatgcgggg
     4921 cggggcgcgg gaaggaccga cagaggcaaa ctcaaccgaa agcaggatcg tcggagcagg
     4981 gacgtcaaac tgtttgaccg tgtcccatgc agaaaagatc ggtccaccga gatcaataga
     5041 accggaagtg tggtgctgaa cccactggca gaggtcggta tccgagtgct cgtgcccatc
     5101 ggtatcagcc gcagccagtt cgtggtgcac gtcctgcgcg accgcaaagg gtgcaatggc
     5161 gagcagcaac aggattagcc cgagggagag gccgcttctc aaccggtttg ataggtttga
     5221 agaattcatc gagggggcag tgagtaccac aataccaaga acactgtcaa acaagagcca
     5281 caacctatgg agtcgaaacc tttatacatc aatgagttac acctatctca gtagacattt
     5341 cttcaacatt tcgttatgct gaccctctta tcggaaacga aaaggctggg cgatgaaccg
     5401 acccctcgac aatcaacacg ttatagaaat caaggcgttg ccatcaccgc gcgccatcaa
     5461 gaccaaactg ccgatcaccg atgatgcagc ggcattggtc gtggaaaccc gcgacgccat
     5521 ccggcgcatt cttcatggcc aggaccgcga gcgactactc gttatcgtcg gcccctgctc
     5581 gatccatgat ccggaatcgg cctatgaata tgccgaaaga ctcaattcag tcgcggacgc
     5641 gctccgcgat cggctcctaa tcgtcatgcg aacctatttc gagaaaccca ggacgacgat
     5701 cggctggaag ggactcatca acgatcccca tttggacggt tcctgcgaca ttgccactgg
     5761 catggaactg gccagaacta tcttattgaa aatcaatcag cgtggcgtac cctgtgcgtc
     5821 cgaattgctc gatccgatca gtccccaata tatcgccgat ttgattagct ggaccgccat
     5881 cggcgcccgc actacggaaa gccagaccca ccgggaaatg gccagcggtg tctccatgcc
     5941 ggtcggcttc aagaatggga cagaagggag cctgcaagtt gccatcaatg ccatgacatc
     6001 ggcccgcacg cctcatcatt tcgtcgggat caatgccgat ggccagacct cgatcattaa
     6061 gactatgggc aaccccgatc gccatatcgt gttgcgcggc ggcggcggaa aaaccaacta
     6121 tgatgctgaa catgtcgccc gtgctgaggc cgctctggcc ggcgaaggca tcgcccgtcc
     6181 tatcatgatt gattgttcgc acgacaattc cagcaaggat cacaaacgcc agggccttgt
     6241 cgcccgcgac gtcctccgcc agttccggga aggacgccaa tccattatgg ggttgatgct
     6301 cgaaagcaat ttgaatccgg gcaagcaaac ctggaaacag ggtatcccgc tcgcctatgg
     6361 tgtctcgatc accgacgcct gcctcgggtg ggacgaaacc gcctcgctct tgaacgagtt
     6421 ggccgatagc atcgtcacga agcccgccta acacgtcgtc tctttgttct gctgcaccaa
     6481 agatcgcgca atcacatgct gattgacacg catacacatc tggatgacgc ccgatacaac
     6541 gacgaccggg acgcgatgat cgcccgcgcg cgcgaagcgg gcgtcggggc gttcgtcacc
     6601 atcggctgcg atctgtcaac cagccgggct gccgttgcac tcgccgatca atactccttc
     6661 gtgtatgcct caatcggagt gcacccccat gaggtgaagc acatcggcga cggctggtac
     6721 gacgatttcc gccggctggc ccggaacaag aacgtcgtgg cctacggcga gatcggactg
     6781 gactatcact acaaccattc ctcaccgaaa gaccagcggg atcgattcag agaacagatt
     6841 cagctcgcac gcgaattgaa acttcccgtc attatccata ctcgcgaagc tcaagaagat
     6901 acgattgcga ttttgaagga agagaaggcg tcagatgtcg gcggagtgtt tcactgcttt
     6961 tccggggatg cctggctggc gaaggatgcg ttggatcttg gattctattt gtccttcgcg
     7021 gggatcttaa cgtttcagaa tgcgacgatg ctgcgcgaaa tcgctaaaaa tacgccactg
     7081 gaccgtgtgc tgatcgaaac cgactgcccc tatttgactc cagtgcccca tcgaggaaaa
     7141 cgcaacgaac cggcctacgt ttcctatgtc gcacaacaac tggccgagat tcacagtgga
     7201 acaaacagtc tctccgccgc tcgcatcgga gagctgacat cggcaaacgc caagcgtctc
     7261 tttaaaatcg cctaagctgt ttgctgcgct gtcgctgcgc tttcggcagc tttcggggat
     7321 tacccttttt ctcctggtcc cgtctcgtgc gagaatcctc ttccgtatcc aattccttgt
     7381 gcgccagcac gccggcaaac tgtgacagcg ctcgcagctt atcggaaaat tcctgagcgc
     7441 ccattatgaa agccccatga gctttggccg tcgccacaat ctcatggtcg gaactcacga
     7501 tcgcgcagtc ggagccatac tcccgtgcca gccgttggat gacttgatcg gcccgctcgc
     7561 cccgtttcga gtagatcacc tggactcccg cacgatgctc tcgcccttcg atcggctggc
     7621 catgctgcca tccgtcaaag accaccgtca cggcatggcc tttccgctgt cgatacgccg
     7681 ccagatcccg gaggagagct tcccgggcca tctcagagtg tagaactccc attcctccga
     7741 tacgggcgag aagattgtac ccatcgatga tcacgtgcat cgccatgcga aaccctaccg
     7801 ccggctaaga cgcactgtca atgaacgctc atctcggact taatgcggct ccccttgaca
     7861 agtttcaaaa catctgagac atagatatga atccctgcaa gtttcgtgaa aatccatgcc
     7921 gttagcctat tggcgtacag cctgtctcat ccttctaacc gggctctcag gcctgcaagg
     7981 tacttttcca gttgttgccg gtctcgacca ggtcctggcc gccacacagg agacgcgccg
     8041 cgagcagcca cgccactcga aacggtcacg cacggaacaa gtcagcctgg ctcccgtgag
     8101 gatgtctccc gccatgatcg agaatctaag gaccgctgtc gagtctggct cgaccaggct
     8161 cgtcctcgac cttgatcgga aaacgcgcgc tcgcaaatac atcccggccc aggctgatgg
     8221 tgtcatcctg gaaattcctc acgcgatcct gagcaagtcg gctcaaacca agattgcaaa
     8281 cgggacgctc gccaagccgt tcgtgatcag acaaacatcg gctgacaccg ttgccgtctc
     8341 gctcccgaac ggcacgttcc acacatacaa attattctcg ctgacgaatc ctccacgact
     8401 cgtcatcgac gtcgccccgt caagccccca ggatttttct cgccccctcg aaccaaccga
     8461 gccctcgcgc ttacccgacc cgccacccca gccgaatcct cctcgtgcga agtccttcac
     8521 gactatcgtg atcgacccgg gacacggcgg gaaggacccc ggggctcacg ggcggcgcgg
     8581 gaccgaagaa aaagacatca cgctcaaagt cgggctgaaa ctgcgcgact tgctcagcag
     8641 gcaaccgggg atccgcgtcc ttatgacccg ggagcgagac gtgttcgtgg aactcgaaga
     8701 tcgcgcgaag ttcgcgaatg gcaacgatgc cgacctcttt gtatcaatcc acgtgaattc
     8761 ccacccgcaa cggtccgtga gaggcataga aatctatcac tttggagaag ccaaggacca
     8821 acgcgccctg gaagtagcag cacgagaaaa cgggactcct ctgagcagca ccggagtcgg
     8881 ctgggaatac ctcgtggccg atctcctgac cgcaaagaag atcgaaacat cgcttgaact
     8941 cgcctggaac accaaagaag ccatgatcac ccacttgaac ggccactatg cgctcgtgga
     9001 ccatggcgta aaaaccgcgc ctttttacgt gcttcgctac acgagcatgc cgagcatctt
     9061 ggcggaaatt gcgtacattt ccaattcagc cgaagaagac ctccttcgaa cgagtgcgtt
     9121 tacgacacgc gtcgccgaat cgctgctcga aggcgtgcag gcgttcctcg tagcatcgaa
     9181 acagcccgcc cgatgaatca tcctgaattt gtcccaacaa tgccatgcca accgtgaagt
     9241 taatcgtcga gtacgacggc tcgcgttatg ccggctggca acgccaaccg gaccaaccga
     9301 cgattcaaga ggctctcgaa accgcaattt atcaattgac gcagcacaag gtctcggtga
     9361 tcggcgcggg ccggaccgac gccggtgtcc atgccctggg acaagtcgtc agttttcgaa
     9421 tcgagcgtga ctggactgct cgtgaatgga ccaggggatt caatgccaga ctgccggatg
     9481 acatcgccgt gcgatctgcg tctctcatgc ccgatgaatt tcacgcgaga tacggtgcct
     9541 gcggcaaact ctatgagtat cgtatcctca accgtccgga gcggccagcc atcgatcgag
     9601 cctatgtgtg gcatgtctac aagccactca acgacgaagc catgcggcag gcggcttcac
     9661 acctgatcgg ctcacacgat ttcacgtcgt ttgaaggcac tctgaccgac aatgaagatc
     9721 cctcctgcaa tctccaacaa ctgtctctga cgcgccaagg cgatcatctg gtgattcaag
     9781 cgtatgcgga tcggtttctc aaacacatgg tccgcgccat cgtgggaacg ttagccgaag
     9841 tcggccattc gaaacgtacg ccacaaagca tgacagaggt ccttgtggcc aaggaccgaa
     9901 cagttgcagg tcggaccgct ccgcctcagg gattattcct cgttcgcgtg gattacaacc
     9961 attaatcgga ttccttacag acatcctgca gcgtttctgc tatatatggc caagcatctc
    10021 gcttctcact tactcacaaa aggagattga ggatgaagat tgccagcctg ttagtcctgt
    10081 tgctctttac gtccgggatc gccctggcgc cgatggccag cgaagccgcg gatcaacaga
    10141 ataagatgca agcctgcaat gaacaggcaa atgcaaaggg attcggcgag ggaaagggtg
    10201 acgagcggaa agcgtttatg aaggagtgtt tgtccgcgaa gccggcgaaa agcgggaaaa
    10261 cagctcagca ggagaaaatg aaggcgtgca acaaggaagc tggcgagaag aaattagcag
    10321 gtgacgagcg gaagaagttc atgagcactt gcctatccaa ctaatcgagc aaccaaccca
    10381 cttcgcagtc agcgcggctc ctcgccgcgc cgactgcatg tccaagtcat acccctgaca
    10441 atcagattca aggcaacgaa cctcccttgt ccggatcatt cgggtacatc cacttcagga
    10501 attttgatgg aacagcaagg ctagcgggac ggcggggtat cctggttctc gagtttcttt
    10561 cggagttcga ttaactcctg ttcgagggca aggagacggt cgctgacggg atccggcagg
    10621 ttgatgtggt ccatcgtggc atcggggagc cgctctccct gggttttgat cacacgagcc
    10681 gggactccga tgacggttga attggcggat acattcttga ggacaacgga attggcgccg
    10741 atcttgacgt tgtcgccgat ggtaattcca ccgagaatct tcgcccccgc tcctacgacg
    10801 acatggttcc ccagcgtcgg gtggcgctta cctcgctcct taccggttcc tccgagcgtg
    10861 acaccctgaa agagcgtgac ataatccccg atctcggccg tctctccgat cacgacgccc
    10921 atgccgtggt cgataaagaa tcccgtgccg atttttgccg aaggatggat ctcaatacct
    10981 gtcagccatc gcgcaaactg cgagatgagc cgagggataa acggcaccct gtgtattttt
    11041 agccaatggg aaatgcggta ggccagcaag gcgtggaagc cggcatacgt aaagatgacc
    11101 tccaaccgac tggtcgcagc cgggtcacga tcgaaaacag cttttaagtc ttggcggata
    11161 gctttaagca tatttggtta gaaagtgtga aagttagcaa gttgtaagtt aagaagttta
    11221 agagcaagaa cccagccacc gaaactttcc aactttgaca cttttcaatt tccccgtgct
    11281 aaataaccga taaatcctga caaggctttt gacaggttct ctgccttcgc cttcacttct
    11341 tcaaagtccg cacggtcaag ataccccaac tccagcgcaa tgtacaattg actgcgcacc
    11401 tcacctgagg aacctttggc aatacgtaaa aatctcgaaa actctttctg tgacccgcgc
    11461 tcaaaacctt ccgcgatgtt cgacatgacc gagacagagg cttttctgat ttggtcacga
    11521 aggccaaagt cctgcgcgaa cttcccgttc ccgctgagcc catagatggc agcgactaac
    11581 tccttggcca tcacccaagc cttgatgtcc tcaaatcgct cgatctttgc caatgccgct
    11641 gcactccaag atgctcaact ttcgaacttt ccaactttca cacatttaac tatcagagcg
    11701 gttcaatcaa acagacggca tgggcggtca tgccctcttc cctgccgaca gcatcgagtc
    11761 cctctccgct tttcactttc acgttgacca ggtccgcatc gatgccggca gcttccgcca
    11821 tcttcttctg cattgcaact agatgtgggc cgaggcgcgg agcctgcgcc acaatgaccg
    11881 tatcgatatt tccgatgcga taccctttga ccttgagctt tgagaccaca tcttccaata
    11941 atttgagact cgagattcct ttgtaccgtg gatccgaact tggatagtgg cggccgagat
    12001 caccctcccc tattgcgccg aggagcgcat cgcagacggc atgaaccaag acatcggaat
    12061 cggaatgccc cagaagaccc ttattgtgag gaatttcgat cccgcccaga atcagtttcc
    12121 gccccggccc aagcggatgc acgtcgtacc cgtagccaat tcgagctcct gttgccatat
    12181 ctatcctttc cccgccaccg actgtcgcga agcgagaatc gcctcgccga tgatcatatc
    12241 ctccggcctc gtcactttga tattctcgcc gctcccttcc acgaccgtca cagagcgtcc
    12301 catccattcg agtagagagg catcgtccgt tgcgggcacc gcttcgatct gcgccctccg
    12361 atgcgcttct tgaagccaat cgcgcctgaa cgcctgcggg gtctgcgcga gccagagcgg
    12421 ccgccgatcg accgtgcgct cgatgagatg atcggcccca acatacttga cggtatcccg
    12481 catcggtagc gcgatgattg ccgctcccgt tgcgcgcgcg gcagccacga cgtcctccag
    12541 catgcgctcg gtgaggaatg gccggacggc gtcgtgcacc accacgatct caacctgatc
    12601 gtcgaggacg gccaacgcat ttcgcacgga atcctgacgc tcctgaccac cggccaccac
    12661 cttgatcact tttgtaaagc ggtgggcatc gacgatatcg gattgacaat aggcaagatc
    12721 ggcctgcgga acggcaagca caatcgcatc gatgacggat gaagcctgga gcgtacggag
    12781 tgaatggacg atgagaggca ggccgccgat cgagagaaac tgcttgggga cggccccgcc
    12841 catgcgaagc ccccggccgg cagcaggaac gagggcgacc gttttcctag acacgcgcga
    12901 cctgcaactc ctctcgctcc gcctcttcct tgaggcgggt aaagatcatg cggccggctg
    12961 tcgtctggag cacgctggtc accaccacat cgacattctt gccgatgtag cgcctcgcat
    13021 tatccaccac a
//
LOCUS       DATIFR010000036         6957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1360213, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000036 DATIFR010000000
VERSION     DATIFR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360213"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..549)
                     /locus_tag="VLD60_01995"
     CDS             complement(136..549)
                     /locus_tag="VLD60_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HSB43749.1"
                     /translation="MAQDDDQEHDRLFTIAEANHLIPQLNTRLTSVRQAKAVLVRTKA
                     DIRRASAKAEYGGGSAAGPLYIASLQQISTNLQAIHELGVVVKDLDQGLCDFPHLRDG
                     RVVFLCWKLGEREIKWWHETTNGYKDRCPLEDSVQ"
     gene            1051..2298
                     /gene="rho"
                     /locus_tag="VLD60_02000"
     CDS             1051..2298
                     /gene="rho"
                     /locus_tag="VLD60_02000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSB43750.1"
                     /translation="MHLAELKQKTIADLNDVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVARDKILFDNLTPLYPEERLNLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALVDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRIFPAIDISQSGTRKEELLVDKDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKVQGTKTNQEFLQSMNR"
     gene            2391..2597
                     /gene="rpmE"
                     /locus_tag="VLD60_02005"
     CDS             2391..2597
                     /gene="rpmE"
                     /locus_tag="VLD60_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HSB43751.1"
                     /translation="MKKGIHPLYREATVHCACGNSYKTRSTVGDINVDICSSCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKGK"
     gene            2698..3780
                     /gene="prfA"
                     /locus_tag="VLD60_02010"
     CDS             2698..3780
                     /gene="prfA"
                     /locus_tag="VLD60_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSB43752.1"
                     /translation="MDAGLLKKWESTASRYDELTMQLTDPSVISQPALLVKLTKERTE
                     IESIAQLFHRYQEILKQLDEAAHMLADPAAGSELHSLATEESAQLEAERRGLEAQVLE
                     HLTPKDPRDEKNLFLEIRAGTGGDEAALFAGDLFRMYVKFAERNKLKVEIVEASETGK
                     GGYKTVIAVIEGKGGYGLFKYESGVHRVQRVPVTEAAGRIHTSTVTVAVMPEVDEVDV
                     QIDPMDLRIDTFCSSGAGGQSVNTTKSAVRITHIPTGVVVSCQDERSQLKNRNKAMRT
                     LRARIVEAEREKQEAEIAQSRKAQVGTGDRSEKIRTYNFPQNRVTDHRVGLTLHKLEQ
                     VLAGDMGELVDALRAQRDHMAVAEAS"
     gene            3777..4691
                     /gene="prmC"
                     /locus_tag="VLD60_02015"
     CDS             3777..4691
                     /gene="prmC"
                     /locus_tag="VLD60_02015"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSB43753.1"
                     /translation="MSLGAANPVSTACFTVGTLIAESRRQLLAAGIESAAQEAVWLME
                     QALNLSGLRQILDHNRELSKRETAEVLALLTRRLKREPLQYLVGTQEFCGLEFEVNPS
                     VLIPRPETELLVRETIRRLPRGQHPTLVDVGTGSGCLAVTLARSIPAGKMLAIDLSAS
                     ALQTAKRNADRHGVGSAIIWSEGDLLAPLAGRGLEGAVTVIVANPPYIREAEWETLQP
                     EVRLYEPRAALIAGPGGTELHERLLNEAIPFLMPGGLLIMELGQGQSAALCEMVESMS
                     AYTSAEIVLDEAGIDRVLIVERADHANG"
     gene            4684..5943
                     /gene="murA"
                     /locus_tag="VLD60_02020"
     CDS             4684..5943
                     /gene="murA"
                     /locus_tag="VLD60_02020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSB43754.1"
                     /translation="MDKIIISGGIPLHGEVRVSGAKNSALPILASTILGGGECVITNV
                     PRVVDVVTMGKLLGMLGVTVEHEGNRAVIKADVIKSTQAPYELVKTMRASVLVLGPLV
                     ARWGEATVSLPGGCAIGSRPVNLHLAGLAKLGAEVSIEHGYIRARAKRLTGARIYCDT
                     PTVTGTENLMMAASLALGTTVIENAAKEPEIVDLADFLSKRGARIAGAGTDVITIEGV
                     RELRGSDHEVIPDRIEAGTHLVAGAITKGCVAVNRCRPDHLDPLLNKLQEAGAEVQVE
                     KERIRLDASKVARLKGVDVRTLPYPGFPTDMQAQMVALMSVAEGTSVVTETVFESRFM
                     HVEELCRMGADIRVEGNRLVITGRDRLTGAPVMASDLRASAGLVLAGLAAEGTTEVLR
                     VYHLDRGYERIEEKLRALGAVIERRKE"
     gene            5947..6651
                     /gene="hisG"
                     /locus_tag="VLD60_02025"
     CDS             5947..6651
                     /gene="hisG"
                     /locus_tag="VLD60_02025"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSB43755.1"
                     /translation="MLTIALCKGKMIEPAIELLARAGYNAVQLATDSRRLVFPCPEIG
                     MTFLIVRPTDVPTYVEYGAADLGIVGKDVLLEQDSDVYEPLDLGFGACRIAVAALQGE
                     ESRDRLSSKIRVATKYPKITERYFNQRGVPVEIIKLYGSIELAPIVGLADRIVDLVET
                     GSTLKAHDLVEVECIARSTARLIVNRASLKLKHKAVTELVRKLRAAGLKAQRSRQAAS
                     PKLSRKADRKGKRTRG"
     gene            6648..>6957
                     /locus_tag="VLD60_02030"
     CDS             6648..>6957
                     /locus_tag="VLD60_02030"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSB43756.1"
                     /translation="MKIVGSTDRGFIPTLKKVAARARVQGAAVEKPVKAILQAVERGG
                     DKAVLRFTKQFDKVSLKPDQLKVTPEEIKEAYFRIRKDEGDALRFAAQRITAFHERQR
                     T"
BASE COUNT         1531 a         1836 c         2156 g         1434 t
ORIGIN      
        1 cacccggcct tgctggtcga gtggatccat cttggctaga tgggctgggc gatagatttt
       61 tcgtttggct tcgccatccg gaccgtcaaa accgagaatg acaaatttca tggctgaaaa
      121 tgtggctttc ctgggttact ggacggagtc ttcgaggggg cagcgatcct tatatccatt
      181 tgtggtttcg tgccaccatt tgatttctcg ctcgccgagt ttccagcaga gaaacaccac
      241 acggccgtcg cggagatgag ggaagtcgca gaggccctga tcaaggtctt tcacgaccac
      301 gcccagttca tggattgcct ggaggttggt actgatctgc tgcaagctgg caatatacag
      361 cggaccggcg gcgcttccac ccccatattc agctttggcg ctggctcttc ggatgtctgc
      421 cttcgttcgg accaagaccg ccttggcctg tcggacggag gtgagccgcg tattcagctg
      481 aggaatcagg tgattggcct cggcgatggt aaaaagccgg tcatgttctt ggtcgtcatc
      541 ttgtgccata ggagactccc gatcccattg gctcctttag cacacccgtc tttgcgcctg
      601 caaccaagtt cccgtgccgc atctttcccg aaatgggtcg gattatgccc atgagttttt
      661 actcaatgac tggaatcggg gggtacttga gatatgtggt ttctctgttg acaaccatcg
      721 tcttgcgcgg tatgcttcac ccatcgtttc aattcgagtc tacgttctct ccacacacaa
      781 aacttccggg gcatctctcg aaccacgcaa tatgacttgg tgagcacagg cggatgagtt
      841 cggctgtagt ttcagtttgc aagaatatcc acgattcgaa gacggatacg tccttcacga
      901 cacatcctct ctagcaaaga cacaatcgcc aattcattgt cgaatcatct attgcgcatc
      961 gtttcaatgt ttgatcacgt tcttatttgt ccttgaccat atagccggga gcgtaagggg
     1021 atacctatgg caaacaccaa gggagaggtt atgcatcttg ccgagttgaa gcaaaaaacc
     1081 attgccgatt tgaatgacgt cgcgcgcgac ctcaagatcg agggcgccgc taatcttcga
     1141 aagcaggaac tgatcttcgc gatcctgcag gcacagacgg agaaaaacgg cgtggtgttc
     1201 ggcgagggtg tgttggaaac cctgccggac gggtttgggt ttctccgggc tcctgactcc
     1261 aactaccttc ccggtcccga tgacatctac atctccccct cgcagattcg ccgattcaac
     1321 cttcgaaccg gtgatatcgt gtccggccag atcaggccgc cgaaggaaag cgagcgctat
     1381 ttcgcgctgc tcaaggtcga aaaggtcaac tatgaagacc cggaagtcgc tcgggacaag
     1441 attctcttcg acaacctcac cccgctctat cctgaagaac ggctcaatct tgagtttgat
     1501 cgtgaagaat attgcacccg tgtcatggat ctcaccacgc cgatcggcaa aggacaacgc
     1561 ggcctcatcg tcgcggcgcc acgcaccggt aagacgatgc tgcttcaggc catcgcccgc
     1621 gccattctga agaaccataa ggaagtgacg ttgatcgtgc tgttgattga cgaacgtccg
     1681 gaggaagtca ccgactggca gcggcaggtc aaagccgaag tgatcagttc cacattcgac
     1741 gaacccgcgc agcgccacgc tcaggtggcc gaaatggtgc tggaaaaagc caaacggctc
     1801 gtcgaacaca agaaggatgt ggtcatcctc ctggacagca tcacgcggtt ggcccgcgcc
     1861 tacaatacga ttgctcctcc gagcggcaag gtgttgtcgg gtggtctcga ttcgaacgcg
     1921 cttcagcggc cgaagcgatt cttcggggcg gcgcgcaaca tcgaaaacgg cggcagcttg
     1981 accattatgg ccaccgcctt ggtcgacacc ggcagccgca tggacgacgt gatctttgaa
     2041 gaattcaagg gcaccggtaa catggaagtc catcttgatc gtcgcctcgc cgacaagcgc
     2101 atcttcccgg ccatcgacat cagccagtcc gggacgagaa aagaagagtt gctcgtggat
     2161 aaggaccggc tcaacaagat gtggatcttg cgcaaggttc tcagtccttt agggaccatg
     2221 gaagccatgg aattcctgat ggataaggtg cagggcacca agaccaacca ggagttcttg
     2281 cagtcgatga atcggtaaga accggcttgt atctggccgt cgagacaggg tacagtgacg
     2341 cgccttcgaa tgggcgaagg ttgacgcatc ggatggtagg agtaggacat atgaagaagg
     2401 gtattcatcc attgtatcgg gaagccacgg tgcattgcgc ctgtggcaac agctataaga
     2461 ccagatcgac cgtcggcgac atcaacgtcg atatttgttc cagctgccat ccgttcttta
     2521 cggggacgca gaagatcgtc gataccgaag gacgtgtcga acggttcaag aaaaagtacg
     2581 cgaagaaggg caagtagcga gccagcagta catagaagag accctgcttc gctgaggggc
     2641 agggtctctt ctttttttgt gcagaatcat gggccacgct cggccggttg aggaaatatg
     2701 gatgccggat tgttgaaaaa gtgggagtct acggcgagtc gctatgacga gctgaccatg
     2761 cagctcacgg acccgtccgt catcagtcag cccgcattgc tggttaagct gacgaaggag
     2821 cggaccgaga tcgaatcgat cgcgcagctg tttcatcgct atcaggaaat cctcaagcag
     2881 ctcgatgaag cagcgcacat gctcgcggat cccgctgccg gatcggaact tcattccctg
     2941 gcaaccgaag aaagcgcgca attagaggcg gagcgacgag gtctggaagc gcaggtgctc
     3001 gagcatctga cgcccaagga cccgcgtgac gaaaagaatc tgttcctgga aatcagagcc
     3061 gggaccggcg gcgacgaggc cgcgttgttt gccggcgatc tgttccggat gtatgtcaaa
     3121 ttcgcagagc ggaataagct caaggtcgaa atcgtcgagg cttcggaaac cggaaagggc
     3181 ggctataaaa cagtaatagc ggtgatcgaa gggaagggcg ggtatggcct gttcaagtac
     3241 gagagcggtg ttcatcgtgt gcagcgcgtc cctgtgacgg aggccgccgg aagaattcat
     3301 acttcaaccg tcacggtcgc cgtcatgcct gaggtcgacg aggtcgatgt gcagatcgat
     3361 cccatggatc ttcgcatcga cacgttctgt tcgtctggag ccgggggaca gagtgtcaat
     3421 acgaccaaat cggcggtgcg gatcacccac attcccaccg gcgtggtcgt cagttgccag
     3481 gatgaacgat cgcaactgaa gaaccgcaac aaggcgatgc gaacgttgcg cgccagaatc
     3541 gtcgaagccg agcgagaaaa gcaggaagcg gagattgcgc agagccggaa ggcgcaggtc
     3601 ggaacggggg atcgaagcga aaagatccgc acctataact tcccccagaa tcgcgtgacg
     3661 gatcatcgag taggactgac cttgcataag ttagagcaag tactggcagg cgacatgggt
     3721 gagctcgtcg acgcattgcg tgcccagcgc gaccatatgg cggtcgcgga ggcgtcatga
     3781 gtctcggcgc ggcgaaccct gtcagcactg cgtgctttac agtcggtacc ctgattgccg
     3841 aaagtcgtcg gcagctcctg gctgcgggga ttgagtctgc tgctcaggag gcggtctggt
     3901 tgatggagca ggcgcttaat ctctcggggt tgcggcagat tctcgatcac aaccgagagt
     3961 tgtccaagcg ggagacggcc gaggtacttg cgctgcttac caggaggttg aagagagaac
     4021 cgctccagta ccttgtgggt acacaggagt tttgcggtct ggagtttgaa gtaaatcctt
     4081 cggtcttgat tccccgtccc gagacagagt tgttggtcag ggaaacgatt cgtcggttgc
     4141 cccgagggca gcatccgaca cttgtggatg tcgggaccgg atccggttgc ctggccgtga
     4201 cgctcgcgcg ctcgattcca gcagggaaga tgctggcgat cgatctttca gcctccgccc
     4261 tgcaaacggc aaagcgaaat gccgatcgtc atggcgtcgg ctcagccatt atttggtcgg
     4321 agggtgatct gttggctccc ttagcggggc ggggacttga aggagccgtg acggtgattg
     4381 ttgccaaccc gccctatatc cgggaggctg agtgggaaac gctgcagccg gaagtgcgtc
     4441 tctatgagcc tcgcgcagct ctgatcgccg gtcctggagg gacggaattg cacgaacggc
     4501 tgctgaatga agcgataccg ttcctgatgc caggcggctt gctgatcatg gaacttggtc
     4561 aggggcaaag tgccgcgctg tgtgagatgg tcgagtcgat gtccgcctat acgtcggcgg
     4621 agatcgttct cgatgaggcg ggcatcgacc gggtgttaat cgtcgaacga gcggatcacg
     4681 caaatggata agatcattat ttcaggtggc atccctctcc atggggaggt gcgggtcagc
     4741 ggggcaaaga attccgcctt gccgattctg gcctcgacga ttctcggagg gggcgagtgc
     4801 gtcatcacga atgtgcctcg cgtggtggat gtcgttacga tgggaaagct gctgggaatg
     4861 ctgggcgtga ccgtcgagca tgaaggtaat cgtgccgtca tcaaggctga tgtcatcaag
     4921 tcgacacagg ctccgtatga actggtcaaa acgatgcgcg cgtccgtcct ggtgttgggc
     4981 cctctggtgg cccggtgggg cgaagcaacg gtctcattac caggcggctg cgccataggc
     5041 tctcgcccgg tcaatctcca tttggccggg ttggccaaac tcggggcaga ggtgtcgatc
     5101 gaacatgggt acatcagggc cagggcgaag cggctgacgg gagcccgtat ctattgcgac
     5161 acgccgacgg tgaccggaac ggagaatctg atgatggcgg cttcgctcgc gctgggcacg
     5221 acggtcattg aaaacgcggc gaaggagccg gaaatcgtcg atctggccga cttcctttcc
     5281 aaacgtgggg cacgaattgc aggggccggc accgatgtta tcacgatcga aggggtgcga
     5341 gaactccgcg ggagcgacca tgaggtgatt ccggatcgca ttgaagccgg gacgcatctc
     5401 gtcgccggtg cgattaccaa gggatgtgtg gcggtcaatc gctgtcgacc tgatcacctc
     5461 gatcccttgc tgaacaagct tcaagaggcg ggcgccgagg ttcaggtaga aaaagagcgt
     5521 atccggctcg atgcgtcgaa ggtcgcccgt ctgaaaggcg tggatgtgcg tacattgccc
     5581 tatcctggct ttccgaccga catgcaggcg cagatggtgg ccctcatgtc cgtggccgaa
     5641 ggcacaagcg tggtgacgga aacggtcttt gaaagccgat ttatgcatgt cgaagagctt
     5701 tgccggatgg gcgcggacat tcgcgtggaa ggcaatcggc tcgtgatcac ggggcgcgat
     5761 cggctgaccg gcgcgccggt gatggcctct gatttgcgtg ccagcgccgg tttggtcctc
     5821 gcaggtctgg ctgccgaagg cacgacggaa gtcctgcgcg tctatcatct ggatcgcggc
     5881 tatgaacgga tcgaagagaa gttacgtgcg ctgggagcgg tcattgaacg gcggaaagag
     5941 tgaacgatgc tgaccattgc gctctgtaag gggaagatga tcgaaccggc gatcgagttg
     6001 ttggctcgag ccggctataa cgcggtgcag cttgcgaccg acagccgccg gctggtgttc
     6061 ccttgtccgg agatcgggat gacctttctc attgtcaggc ccaccgacgt gccgacgtat
     6121 gtggaatacg gggcggcgga cctcgggatc gtcgggaaag atgtgttgtt ggaacaggat
     6181 agcgatgtgt atgagccatt ggatttaggg ttcggagcgt gtagaattgc cgtcgccgcg
     6241 ctccagggag aagaatcgcg cgaccggctc tcgtcaaaaa tccgggtcgc caccaaatac
     6301 ccgaagatta cggagcgtta tttcaaccag cgcggtgtgc cggtcgaaat catcaagctc
     6361 tatggctcga tcgaactggc acccattgtc ggattggcgg atcggatcgt cgatttggtc
     6421 gaaaccggca gcacgctgaa ggcgcatgat ttggtcgagg tggagtgcat cgcccggtcc
     6481 accgcgcgtc tgatcgtcaa tcgagccagc ctcaaattga aacacaaggc cgtgacggag
     6541 ctggtcagga agctccgggc cgccggtctc aaggcgcaac ggtctcgtca ggccgcttca
     6601 ccgaagttgt cgcgtaaggc ggaccgcaaa gggaaacgga cgcgcggatg aaaattgtcg
     6661 gctccaccga tcgtggcttc atccccacgc tcaagaaagt ggctgcacga gcccgggtac
     6721 aaggggcggc ggtcgagaaa ccggtcaagg cgattctgca agccgtcgaa cgaggcggcg
     6781 ataaggccgt tctccgcttc accaaacagt tcgacaaagt ctcgctcaag ccggatcagc
     6841 tcaaagtcac tccggaagaa atcaaagaag cctacttccg tattcggaaa gacgaagggg
     6901 atgcgctgcg cttcgccgcc cagcgaatta cggcttttca cgagcggcag cggacca
//
LOCUS       DATIFR010000037         3210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_402587, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000037 DATIFR010000000
VERSION     DATIFR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402587"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..2574
                     /gene="uvrA"
                     /locus_tag="VLD60_02035"
     CDS             67..2574
                     /gene="uvrA"
                     /locus_tag="VLD60_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSB43757.1"
                     /translation="MGNSIVIKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLFARVGRPTCFQCGEEITAQTVQQMVDAIAALPEGVKFQVLAPIVRGRK
                     GEYRKELLEMRRAGYVRARIDGKLVDLGDDIALDKQRKHSIEIIVDRLVMKPGDALTR
                     RLADSVETSLKLAGGLVGVLAEDGKTALYSEKLACIRCGVSYPEVTPRIFSFNSPHGA
                     CPACDGIGYAMTPGCPEEEDLTLLERCEVCKGARLRPESLSVKIAKKSIAEVTQLSVR
                     TAADFFSSLKFTDREMVIAHRILKEIRERLGFLVNVGLDYLTLDRAAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVVVVEHDAETMMA
                     ADHILDMGPGAGSHGGHVIAQGTPQQVMGDPNSLTGQYLRSVQTVAVPQRQRKPKGFL
                     SVVGAQKHNLKNVTAKIPLGLFTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKELHGVLALDKVIDIDQSPIGRTPRSNPATYTGLFGYIRDLYSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLEILHKEKSIADV
                     LNMTVDDALEFFEHIPLIKTKLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RATGRTMYILDEPTTGLHFADVQRLLDVLDRLVEAGNTVLVIEHNLDVIKNADWIIDL
                     GPEGGDRGGEIVAEGSPREIAKSKRSYTGQVLKEAGV"
     gene            3025..>3210
                     /locus_tag="VLD60_02040"
     CDS             3025..>3210
                     /locus_tag="VLD60_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSB43758.1"
                     /translation="MLGDQFKGQWSQFKGELKKQWGKFTDDDLMQIEGDYDKFIGSLQ
                     KRYGDQKEEVRRWVEQWY"
BASE COUNT          752 a          867 c          924 g          667 t
ORIGIN      
        1 agggtggtag cacctgcttt ctcgctttgt caaggcaagg aggccggatg gtataatccg
       61 ccgcctatgg gcaactcaat cgtcatcaag ggtgcgcgcg agcataatct caagaatatc
      121 gacgttgaga ttccgcgcga caaactcgtc gtcatcacgg gcctgagcgg atcggggaaa
      181 tcctcgctcg ccttcgacac gatctatgcc gaaggccagc ggcggtatgt cgaatccctc
      241 tctgcctatg cccgccaatt tctcgagcag atggggaagc cggacgtcga ttcgatcgag
      301 ggactgtctc ccgctatctc cattgaacag aaaagcacca gccataatcc ccgttcaacc
      361 gtcggcaccg tcaccgagat ttacgactat ctgcggctac tatttgcccg tgtcggccgg
      421 cccacctgct tccagtgcgg cgaggagatc accgcccaaa ccgtgcagca gatggttgat
      481 gcgatcgcag cactcccgga aggagtgaag tttcaagtcc tggccccgat cgtccgaggc
      541 cggaaaggcg agtatcgcaa ggaattgttg gagatgcgtc gcgcgggcta tgtccgtgcc
      601 cgtatcgatg ggaagctggt cgatctcggc gacgacatcg cgctcgataa acaaagaaag
      661 cacagcattg aaattattgt cgatcggctc gtgatgaagc ccggcgatgc gttgacgcgc
      721 cgcttggcgg attccgtcga aacatcgctg aagctggccg gtgggttggt gggtgttctt
      781 gccgaggacg gcaagaccgc cctctacagt gagaaactgg cctgcatccg ctgcggcgta
      841 agttatccgg aagtcacgcc tcgtatcttc tccttcaata gcccgcacgg agcctgccca
      901 gcctgcgacg gcatcggcta tgccatgacg ccaggatgtc ccgaggagga ggatttgacg
      961 ctgcttgagc gttgtgaggt ctgcaaaggg gcacgattga gaccggaaag cctctcggtg
     1021 aaaattgcca agaagtcgat cgccgaagtc acgcagttgt ccgtgcggac cgcagccgac
     1081 ttcttttcat cactcaagtt caccgaccgt gagatggtca tcgcccatcg gattctgaaa
     1141 gaaattcgcg agcgcctggg atttctcgtg aacgtcggtc tcgattacct cacccttgac
     1201 cgtgcggcag ccactctttc gggcggggaa gggcagcgca tcaggctggc tacacagatc
     1261 gggtccggcc tcgtgggcgt tctgtatatt cttgatgagc cgtccatcgg gttacatcag
     1321 cgggataatc gccgcctcct ccagacactg ctgcgcctgc gagacctcgg caatacggtg
     1381 gttgtcgtcg agcacgatgc ggaaaccatg atggcggcgg atcacattct cgacatggga
     1441 cccggtgccg ggtctcatgg ggggcacgtc attgcacagg ggacgccgca gcaggtcatg
     1501 ggcgacccca attcgctgac ggggcaatat ttacgcagcg tccaaactgt tgccgtgccg
     1561 caacgacagc ggaagccaaa ggggttttta tccgtcgtcg gcgcgcagaa acataatctg
     1621 aaaaacgtga cggcgaagat tccgctgggg ctgttcacct gtgtgacggg ggtatcagga
     1681 tccggcaaga gcacgctcgt gctcgaggtg ctgttccact cgctctcgca gttgctctac
     1741 cataagaagc cgaagatcga tggatgcaag gaactccatg gcgtactggc gctcgacaaa
     1801 gtgatcgaca tcgatcaatc gccgatcggc cgcacgccac gctcgaatcc ggccacctat
     1861 acgggcctgt tcgggtacat ccgtgatctc tattcaaacc tgccggaatc tcgcgtgcga
     1921 ggctacaaac cgggccgcta cagcttcaat gtcaaaggcg gacggtgcga agcctgccag
     1981 ggtgacgggc tcatcaaaat cgagatgcat ttcctgccgg acgtctatgt gacctgcgaa
     2041 gtctgcaagg ggcaacgcta caaccgggaa acgcttgaga ttttacacaa ggagaaaagc
     2101 attgccgacg tgctcaatat gaccgtcgat gatgcgctcg aattcttcga gcatattccc
     2161 ttgatcaaga ccaagctgca aaccctgcac gatgtcggac tgcactatgt gaagctcggg
     2221 caatcggcga cgactctgtc cggcggcgag gcacagcggg tgaaactttc ccgagaactt
     2281 tcaaaacggg cgaccggacg gacgatgtac atcctcgacg aaccgaccac cggcctgcac
     2341 tttgccgacg tgcaacgatt actcgatgtg ctggatcgtc tggtcgaagc cggaaacacc
     2401 gtgctcgtca tcgagcacaa tctggacgtg atcaagaacg ccgattggat catcgatctc
     2461 gggccggaag gcggcgatcg aggtggcgag attgtagcgg agggatcacc aagagaaatt
     2521 gccaagtcga agcggtcgta tacggggcaa gttttaaagg aggctggagt ataaaagtca
     2581 actgccaatc ttgacggatc accagtaggg taatatcaaa ttgttgagag gaagaccgtt
     2641 cgacgttatc ggatcgtcat cacaaatctt ttctcgcaaa gagtccaatt caatccttcc
     2701 gttttgatct ccctcgacgc aacggccccg ggctcatagc gtcctcaccg agacatcctg
     2761 tcccagtctc caaatacctg gaacgataat gagtttgttc cgttgagcgg gaatatcaac
     2821 cggaaaaaaa gtctcaccgg aagctgagcg ccgttcttcc gttatcctcg gaaacttacc
     2881 agtttcgcgt tacagattgg gaagttagag gggatttgcc atcttgccgg aaatctggtc
     2941 cgtcatttgc gcaactgttc acgtaacggg caagagaaaa tgggcagatt aatcatccta
     3001 aaacctaatc taaaaggggg agttatgctt ggcgatcaat tcaaaggcca atggagtcag
     3061 ttcaagggag agcttaagaa gcaatggggt aagtttaccg atgacgatct gatgcagatc
     3121 gaaggcgact acgacaagtt catcggctca ttgcagaaac ggtacggaga tcagaaagag
     3181 gaagtccgtc gttgggtcga acagtggtac
//
LOCUS       DATIFR010000038         8662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1169629, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000038 DATIFR010000000
VERSION     DATIFR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169629"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VLD60_02045"
     CDS             complement(<1..333)
                     /locus_tag="VLD60_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43759.1"
                     /translation="MREIILGGLKTRLTGGTDGIGGGKGPVVVLLHGFGAPGDDLVPL
                     AEWIAAPRGTRFLFPEGPLSLSMGFGDSRAWWIIDMARLEADRAAGRVRDLSQEVPRG
                     LPQAREALE"
     gene            complement(341..3586)
                     /locus_tag="VLD60_02050"
     CDS             complement(341..3586)
                     /locus_tag="VLD60_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43760.1"
                     /translation="MANKPGDSKLAVAGALTLVLAIAGVLLVKEPLRSSRPVGTGLEM
                     KQSTGEQLVRARLWEDPVAAVERAMREKGSTKAASATSGEPSLAHRLRPLRQAIVERV
                     KNGQRLTVLLVTTSGGPYVESTESRIRDRYAIGTALGVACYAPEEEGHLAFIEWDPNG
                     SVRGLPYEWYSLRKTRVCGGAGSRADSVLVVWLPDEALSRGFFATLTSLSQGLVCQET
                     SRNECLVTEDKRRLVRLNASVQQSVTFKIIGPRSSSAYRALLDEAGKLYGDPHEGIGV
                     WPNADGSIELYSPWASAMKGLLAYGLKSESGKGAACATYEDCEHEFYRRLADVNLRLV
                     YDIGSDDQRFEALIAELERRQVRLGWDAVILIGEWDSFYGRTLPIEFRAAACAKVATF
                     SEADLKQILVPVDIKNWCPTIPRAVDLQIQRPADYESLTLNVFRYSYLGGLDGEIPGD
                     DAARAARAAKATAGDQSKDLTRDRPEGTSQLDYVRALVARIHDEGEGARAIGILGTDP
                     YDALLIIKALRPFFPHAIFFTIDLDARHLHPSEYHSTRNMVVASPFGLQLDGGLQRDV
                     PPFRSSYQTSAYFAVLQAVDHVLCELHGHQRQAGACTTEYHVSLTPANRLYDAGSHPR
                     LYEVGRDGAVDLSVVEKEGVRTVHPLRADLAHTDEYGQLKQGVGFDNTAVAAIVVIGL
                     LIMTVVAWSNQRLWLWVTRNPRLLGIVAIILLASFAAFVALGGATALLAGHDEGEPFS
                     WTAGVSVWPSELIRLFVVVLCLVLLGKGSRDLAKNSDAISEDFLFQVESGPQSFLLAS
                     FWTNLKRVAHPAATMTATTVDQAWAWYREASKPAQWITRTVLLFVLYIGMIWSLGYYV
                     LDEEYIHPCRGQLSCTVDWFLTLSSTVLVVLLNLAVFDAVMLCRRWIGWVTASTGGWS
                     DQVQEEYLRDYGLGQAQKAEFEKLKYLAVVDLIGQRTEVVNRLIRYPFIALLIMIAGR
                     NDYFDIWNYPVLLLLSWALNVILALLAAYLLYQAASGAKAAMLAGLSRQMVQALGTGQ
                     DHDVRMKQVQFIIEEVEANQQGAFVPLYQQPVIESSLYGLVALLQYLYTHH"
     gene            complement(3662..5140)
                     /locus_tag="VLD60_02055"
     CDS             complement(3662..5140)
                     /locus_tag="VLD60_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HSB43761.1"
                     /translation="MTRHSLETLAFDNSYARLPEAFYAKLNPTPFSSPPYLVHANRAV
                     AELIDLDPDEFKRPEFAGVFGGSLLVPGMQPLAMLYSGPQFGVYVPQLGDGRAIMLGE
                     ARNGRGERWELQLKGAGLTPFSRDGDGRAVLRSTIREYLCSEAMHGLGIPTTRALCIV
                     GSDHQVYREQVETAATLLRMAPSHVRFGSFEVFYYRQQHEQLRILADYVIGNYYPHLV
                     DASDKYAGLFNEVMERTAKLIAQWQAVGWAHGVMNTDNMSILGLTLDYGPFGFIDDYD
                     PGFICNHSDHNGRYAFNQQPFIGLWNLSCLAQALLPIIPKEELKVALDRYTPIHQTQY
                     LDLMRSKLGLEGAGAAADEPLVNDLLVLMAESHADYTIFFRALGDFCSAAHEQNEGLR
                     DFFVNRDGFDGWAARYRACLLEQGGTDAERRERMNKVNPKYVLRNYLAQTAIEKAQQK
                     DYSEIDRLLFLLHDPFTDHAGMDSYAAAPPNWGKHISVSCSS"
     gene            complement(5159..5806)
                     /locus_tag="VLD60_02060"
     CDS             complement(5159..5806)
                     /locus_tag="VLD60_02060"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSB43762.1"
                     /translation="MEKLVKGFLKFRTEVFGKKKALFTRLSENQTPRALFITCSDSRV
                     DPTLLTQTDPGELFILRNAGNMVPPYGSMQGGSTATIEYAMAVLKVPHIIVCGHTDCA
                     VMKAVLHPEEVHELPAVKEWVGQAETTRRLMVEHYTNLAGKDRLIKTTQENVRSQLDH
                     LRTHPSVALQLRKGKVDLHGWVYSISTGDVWVYDFESERFTSLLDKNSHRLKAAR"
     gene            complement(5818..6168)
                     /locus_tag="VLD60_02065"
     CDS             complement(5818..6168)
                     /locus_tag="VLD60_02065"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HSB43763.1"
                     /translation="MENAIRNAIIKFEQEFMGRGPDDVRASIVRDLVVVRLKGVLTPA
                     ERQLAKTAEGVDMVKRLRQNLIAQGRDKLCDQVSEIMGARVLGVFTDIDVQLGERVFV
                     FTMDRDMQTSGRTG"
     gene            6541..8250
                     /locus_tag="VLD60_02070"
     CDS             6541..8250
                     /locus_tag="VLD60_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSB43764.1"
                     /translation="MSMFAVLLVPLLPLLTTFIVMAGGDHVSRARAKLAAWPIGVSFC
                     GAIVTLYAVATQGPIAIRLYDTDAILSLPVPFGFYIDRLSAVMMVLISAIGTVIYTYS
                     IDYMYQEPHERRYLAMLGVAVFVLLCMVSSANLLMLFIFWQILSYLLYILIHNHTHPG
                     TLEGAFRTFTMLRIGDVAFLAGIALAYQLYGTLEFPELFAAAAAQSTLMFSLLPGVEL
                     NGATAVTLLLFVGGMSKSAQFPLHIWLPRYLYAPTPVTALLHAGIINAGGFLVNRLAP
                     LFGLSSTTLHVAFLVGTLTAVLGASMMLAQNDIKNMLGFSTIGQMGYMVMECGLGAFS
                     LAVFHLIAHGLFKATVFLNCGNVIDRARLEPHFPHPKRENEEEGRSNLSWLTGFVTTL
                     LLPLLILLLTHGALQIPLLESQGTVIFLFFIWITSSQAILTLTRLRQIESWKVAATML
                     LTLLFVVFVYLFAVESFTAFLYPDPNDVASYFEAAELPGRLFDMMVGAATLTTILGWC
                     YLYMKAHGWSLPAPVWINAIRTRLYVLLLNRLYVDECFHRVAQAQRSALRRVENSAQE
                     RSS"
     gene            8378..>8662
                     /locus_tag="VLD60_02075"
     CDS             8378..>8662
                     /locus_tag="VLD60_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSB43765.1"
                     /translation="MTLVLLVPAFLLVAAAIVLVGSDRTSHARAKLAAYPVGLASLGA
                     MGTLYQVASDGPIEIRFYDLASVASFAIPIGFYVDRLSAIMMTLITGVSVI"
BASE COUNT         1674 a         2628 c         2509 g         1851 t
ORIGIN      
        1 ctccagagct tcgcgcgctt gcggcagccc cctgggcact tcctgagaca gatcgcgcac
       61 acgtcctgcc gcgcgatccg cttcgagccg ggccatgtcg atgatccacc aggcccgtga
      121 gtccccgaaa cccatgctca atgaaagcgg cccttcgggg aatagaaacc gcgtgcctct
      181 cggggccgcg atccactctg ccagagggac cagatcgtcc cccggcgcac cgaatccatg
      241 caggagaacg acgacggggc ccttgccgcc tcctatgccg tcggtgccgc cggtaagtcg
      301 tgttttcaag ccgcccagta tgatttctcg catgggcggt ttaatgatgg gtgtataggt
      361 attggagcag tgccacgagg ccgtagagag aggactcgat caccggttgc tgatagagcg
      421 gtacgaaggc accctgttgg ttggcttcca cttcctcgat gatgaactgg acctgcttca
      481 tccgcacatc gtgatcctgg cctgttccca aggcttggac catttgccgg ctcagcccgg
      541 ccagcatggc ggctttcgcc ccgctcgccg cctggtacag cagatacgcg gccagcagcg
      601 ccaggatcac gttcaacgcc catgagagca gcaagaggac cgggtaattc cagatatcga
      661 agtagtcgtt ccggcccgcg atcatgatta acaaggcgat aaagggatac cggatgaggc
      721 gattcaccac ttcggtccgc tgcccgatca gatcaaccac agccaggtac ttgagctttt
      781 cgaactcggc tttctgggct tgacccagac cgtagtcgcg gagatattcc tcttgcacct
      841 gatcggacca gccgccggtc gaggccgtca cccagccgat ccagcggcgg catagcatca
      901 cggcatcaaa caccgcaaga ttgagtaaca cgaccaagac cgtgctgctc agcgtcaaga
      961 accaatcgac cgtgcagctg agctgaccgc gacagggatg gatgtactcc tcatccagta
     1021 catagtaccc caatgaccat atcatcccga tatagagaac aaacaacagg acggtccgcg
     1081 tgatccactg tgccggcttg cttgcctcgc ggtaccaggc ccaggcctga tccaccgtcg
     1141 tcgcggtcat tgtcgcagcc ggatgggcaa cccgcttaag gttggtccag aagctcgcga
     1201 gtaaaaagct ctgggggccg gactcaacct ggaataagaa gtcctcgctg atggcgtcgc
     1261 tgttctttgc aaggtcgcgc gatccttttc cgaggagaac cagacagagc accacaacga
     1321 ataggcggat caactcgctc ggccagacgc tgaccccggc agtccaggag aacggctcgc
     1381 cttcatcgtg gccagcgagc agcgcagtcg cgccgcccaa tgccacgaag gccgcgaacg
     1441 aagccaggag gatgatcgcg acgatcccca ataaccgcgg attcctcgtc acccaaagcc
     1501 agaggcgctg attgctccaa gcaaccacgg tcatgatcaa gagcccgatg accactatgg
     1561 ctgccactgc cgtattgtcg aatccgaccc cttgcttcag ctgtccatat tcatcggtat
     1621 gagcgagatc cgcccgcaga ggatggaccg ttcgcacccc ttccttctcc acgacgctca
     1681 agtccacggc tccgtcacgc ccgacttcgt acagcctcgg atgagacccc gcgtcataaa
     1741 ggcgattcgc cggtgtcagt gagacatggt attcggttgt gcaagccccg gcctgccgtt
     1801 gatgaccgtg cagctcacag aggacgtggt ccacggcctg cagaacggcg aaataggctg
     1861 aggtttgata gctgctgcgg aagggcggga catcgcgctg gaggccgccg tccagctgca
     1921 gaccgaacgg agaggcgacg accatattgc gcgttgagtg atactcgctc gggtggaggt
     1981 ggcgcgcgtc aagatcgatg gtaaaaaaaa tcgcatgcgg gaaaaacggg cggagggcct
     2041 tgatgatgag cagcgcatcg tacggatccg taccgaggat accgatagcc ctggcgcctt
     2101 ccccttcatc atgaatgcga gcaaccaggg cgcgcacata atcgagttga ctggttcctt
     2161 ccgggcgatc ccgcgtcaga tccttagact gatccccggc ggtcgccttg gcggctcggg
     2221 cagctcttgc cgcgtcatcc cccggaattt ctccgtccag accgccgaga tagctgtagc
     2281 gaaagacatt cagcgtgagc gattcatagt cggctggtcg ttggatctgc agatcgacgg
     2341 cacgagggat cgtcggacac cagtttttaa tgtcgaccgg caccaggatc tgtttcaggt
     2401 cggcttccga aaacgtcgcc accttcgcgc aggctgcagc gcggaactcg atgggcaagg
     2461 tcctcccgta gaatgaatcc cactccccga tgaggatgac ggcatcccag ccgagccgta
     2521 cttgtcgccg ctctaattca gcgatgagtg cttcaaaccg ttggtcgtcg gatccgatat
     2581 cataaacaag tcgcaggttc acatcggcca gccggcgata aaactcatgt tcgcaatcct
     2641 cgtacgttgc gcaggccgct cccttgccgg actctgactt gagcccgtag gccagcaacc
     2701 ccttcatggc gctggcccat ggtgagtaga gctcgatcga cccgtcggca ttcggccaga
     2761 cgccgattcc ttcgtgtgga tcgccataca gctttccggc ctcatccaga agcgcgcgat
     2821 aggccgatga actgcggggg cctatgattt tgaacgtgac ggactgctgc accgaagcat
     2881 tcaaccgcac cagcctgcgt ttgtcttcgg tgaccagaca ctcattccgg ctggtttcct
     2941 gacagacgag cccttgagaa agcgatgtca acgtcgcgaa gaatccgcga ctcaaggcct
     3001 catcgggcaa ccagacgacg aggacgctgt ccgcacgcga ccctgctcct ccacagacgc
     3061 gcgtcttcct gaggctgtac cattcgtagg gaagcccccg aacggatcca ttggggtccc
     3121 attcgataaa ggcgagatgg ccctcctctt ccggagcgta acaagcgacc ccgagcgcgg
     3181 tgccgatggc gtaccgatcg cggatacgcg attcggtgct ctcgacgtag ggacccccgc
     3241 tcgtggtcac cagaagcacc gtgaggcgct gcccgttctt cacccgctcg acgattgctt
     3301 gccggagcgg cctgagccgg tgggccaggg acggttctcc tgacgtcgcg gaggccgctt
     3361 tggttgatcc cttctcccgc atggctcgtt cgaccgccgc cacagggtct tcccacaaac
     3421 gcgcgcggac caactgctcg cccgtggact gcttcatctc gagcccggtg ccgacgggtc
     3481 gcgagctgcg caacggctct ttcacgagca acacgccggc aatggccagc acgagcgtga
     3541 gcgctcccgc tactgcgagt ttcgaatcgc ctggtttgtt cgccatgatg cctgtttcct
     3601 agtctggaca gccccgccac tctacaacag acaataaggg ggactgcaag gtgggaatgc
     3661 gctatgagct acaactgact gagatatgct tgccccagtt tggaggggcc gcggcgtagg
     3721 agtccatgcc ggcatgatcg gtaaatggat catgcagcag gaagaggagt cgatcgattt
     3781 cggaataatc tttctgctga gccttctcga tcgcggtctg agccagatag ttgcgcaaca
     3841 cgtatttggg attgaccttg ttcatacgtt cccgtcgctc ggcgtcggtt ccgccctgct
     3901 caagcaggca tgcgcgatag cgagcggccc agccgtcaaa cccgtcccga ttcacgaaga
     3961 aatcgcgaag gccttcattc tgttcgtggg ccgctgagca aaaatctccg agcgcgcgga
     4021 agaagatcgt gtagtccgca tggctctccg ccatgaggac caagagatcg ttcactagag
     4081 gttcatcggc ggcggcgccg gccccttcaa ggcccaactt gcttcgcatg agatccagat
     4141 actgcgtttg atgaatcggc gtgtagcggt cgagcgcaac cttcaactcc tctttcggaa
     4201 taatcggcaa gagcgcctgg gccagacagc tgagattcca cagaccgatg aagggctgct
     4261 gattgaacgc gtatcttccg ttgtgatccg agtgattgca gatgaacccg ggatcgtagt
     4321 cgtcgataaa gccaaatggg ccgtagtcga gcgtgaggcc gaggatcgac atattgtcgg
     4381 tattcatgac accgtgggcc cacccgacag cttgccattg tgcgatcaac ttcgccgtcc
     4441 gctccatgac ctcgttaaag agcccggcgt atttgtctga ggcatcgaca agatgcggat
     4501 agtagttccc gatcacataa tcagccagga tcctcaattg ctcgtgttgc tgccggtaat
     4561 aaaagacttc aaaggacccg aaccggacgt gtgatggggc catgcgcagc aaggtggccg
     4621 cggtctcgac ctgctcgcga tacacctggt gatcgctgcc gacaatgcag agtgcgcgcg
     4681 tggtaggaat gccgaggccg tgcatcgctt cgctgcagag atattctcgg atggtcgagc
     4741 gcaggaccgc ccgtccatcg ccgtctcgtg agaacggcgt gaggcctgcg cctttcagtt
     4801 gcagttccca gcgttcgcct cgtccgttcc gcgcttctcc caacatgatc gctctgccgt
     4861 cacccagttg cgggacatag acgccgaact gaggcccgga atagagcatc gccaacggtt
     4921 gcatgccggg caccagcaga cttccgccga agacgccggc gaactccggt cgcttgaact
     4981 cgtcgggatc gagatcgatc agctcggcta cggcacgatt ggcgtgcacg agatagggag
     5041 gggaactgaa cggtgtcggg ttcagcttgg cgtagaaggc ctcgggcagg cgggcatagc
     5101 tgttgtcgaa cgcgagcgtt tcaagggagt gtctcgtcat gaacgaagca tgacaggact
     5161 agcgagcggc tttcaagcga tgagagttct tgtccaacag ggacgtaaat cgttcgctct
     5221 cgaagtcgta gacccagacg tcgccggttg aaatggaata gacccatccg tggagatcga
     5281 cctttccttt ccgcagctga agggcgactg agggatgtgt gcgaagatgg tcgagttggg
     5341 atcggacatt ctcctgcgtc gtcttgatca ggcggtcttt gcctgcgagg ttggtatagt
     5401 gctcgaccat caagcgtctc gtcgtttcgg cctgcccgac ccattctttg accgccggca
     5461 actcatggac ttcttcggga tgcagcacgg ccttcatcac ggcgcaatcg gtatggccgc
     5521 agacgataat gtgggggact ttcaacaccg ccatcgcata ctcaatggtg gccgtgctgc
     5581 cgccttgcat ggagccatag ggcggcacca tgttgccggc attccggaga ataaagagtt
     5641 ccccgggatc tgtttgtgtg aggagcgtgg gatcgacccg cgagtccgaa caggtgatga
     5701 agagggcgcg aggggtctga ttctcagaga gcctggtaaa aagcgccttc ttctttccga
     5761 acacttcggt gcgaaacttc aggaagcctt tgacgagttt ttccatcgac ggcgtctcta
     5821 tcctgtgcga ccgctggttt gcatgtcccg atccatcgtg aacacaaaga cccgttcacc
     5881 gagctgcacg tcgatgtcgg taaagactcc aaggaccctc gcgcccataa tctcgctcac
     5941 ctggtcgcag agcttgtccc gcccctgcgc aatcaaattc tgccgcagac gcttcaccat
     6001 gtcgacacct tctgcggttt ttgccagctg tcgttccgcc ggtgtgagga cgcccttcag
     6061 gcggacgacg accaagtcgc ggacgattga ggcccgcacg tcatcgggtc cccggcccat
     6121 aaattcctgt tcgaatttga tgatcgcatt gcgtatcgcg ttttccatga tcgctccggt
     6181 tgcgttggat tataggattg cgcctgagcg gtgaacaagt aggaatgtaa gcccttcgta
     6241 tcacgaccac catctgttct aggcctttcg ggctattgcg cggcgttatt gagacccgag
     6301 atgcaccgcc gttattgacg gtaaggttgg taccgtatta ttgatgaagg cgtgcggcat
     6361 gagagggcgc tgctgattgc cccgaccatg tgtcgaggat aaacgggacg ccgagttttt
     6421 ctgataggcc gtagggtgac cggctcttcg gtgcagaggc cggtcgcagt acaagccaac
     6481 cgagccgcgt cgatcttaca tcgacggacc ggttggcttt cgtttttttg gggtcgcgac
     6541 atgtcgatgt tcgccgtcct gcttgtcccg ttgcttccgc tgctgaccac gttcatcgtg
     6601 atggccggcg gcgatcacgt cagccgtgca cgcgcgaagc tcgccgcttg gcccatcggt
     6661 gtctccttct gcggcgcgat cgtcacgctg tatgccgtgg cgacgcaggg accgatcgct
     6721 atccggctct acgatacaga cgcaatcttg tctcttccgg tcccgttcgg tttctacatt
     6781 gatcgactca gcgccgtgat gatggtacta atctccgcga tcgggacggt catctatacc
     6841 tactccatcg actacatgta tcaggagcct cacgaacgcc gctatctagc catgctcggt
     6901 gtcgccgtct tcgtgctcct ctgtatggtg tcgagcgcca atctcctcat gttgtttatc
     6961 ttctggcaga tcttgagtta cctgctgtac atcttgatcc acaaccatac ccatcctggg
     7021 acgctggaag gcgcctttcg tacctttacc atgttgcgaa tcggggacgt cgcctttctc
     7081 gcggggatcg cgctggccta tcagctgtac ggcacgttgg agtttccgga attgtttgcc
     7141 gccgccgccg cgcaatccac cttgatgttc tcgctgttgc ccggcgtaga actgaatggt
     7201 gcgacggcgg tcaccctgtt gctgttcgtc ggaggcatga gcaagtctgc acagtttccg
     7261 ctgcacattt ggctgccgcg ctatctatat gctcccacgc ccgtcacagc cctgctgcac
     7321 gccggcatca tcaacgccgg cgggttcttg gtcaaccgcc tggccccgct cttcgggttg
     7381 agttccacga cgctgcatgt cgccttcctc gtgggtacgc tcacggcggt cctcggcgcc
     7441 tcgatgatgc tggcgcagaa cgacatcaag aacatgctcg gtttttcgac gatcgggcag
     7501 atgggctaca tggtcatgga atgtggcctc ggggcgttct cgctcgccgt cttccatctg
     7561 atcgcgcacg ggctcttcaa ggcaaccgtg tttttgaatt gcggcaacgt catcgaccgc
     7621 gcgcgcctgg aaccccattt cccccatccc aagcgagaga acgaagagga gggccgctcc
     7681 aacctgagct ggctgacagg attcgtcacg accctgcttc ttccccttct catcttgttg
     7741 ctcacgcatg gagccttgca gattccgctt ctggaatcgc aaggcacggt gatcttcctg
     7801 ttcttcatct ggatcacttc ctcgcaagcc attttgaccc tgacgcgtct gcgccagatc
     7861 gaatcatgga aagtggcggc gaccatgctc ctcacactct tattcgtcgt gtttgtctac
     7921 ttgttcgcgg tggagtcgtt tacggccttc ctctaccccg atccgaacga cgtcgcgtca
     7981 tacttcgaag ctgccgaact gccgggtcgg ctcttcgaca tgatggttgg cgcggccacg
     8041 ctgacaacaa tcctcggctg gtgctacctc tacatgaaag cgcatggctg gtcgctgccg
     8101 gcgcccgtgt ggatcaacgc catccggact cggttgtatg tgctcctgct caatcgtctc
     8161 tatgtggacg agtgcttcca tcgcgtggcc caggctcagc ggtctgcgct tcgacgggtc
     8221 gagaactcag cgcaagaacg gtcgtcgtga gcgatgcgct tgacgaagct ccggaggtcg
     8281 tattaatggt tagcccgccg tcgataggcc ggacggggtc cggcactgct ctgaagcagc
     8341 gagactgcga tcatgctggg agagtgaaga ggtgtccgtg acgctcgtcc tgcttgttcc
     8401 ggcgttcctc ctcgtcgctg ccgccatcgt gctggtcggc tctgaccgca caagccatgc
     8461 gcgcgcaaaa ctcgctgcct atcccgtcgg tctcgccagc ctgggggcca tggggacgct
     8521 ctatcaggtc gccagcgacg gtccgataga gattcggttc tacgatctgg cctcggtcgc
     8581 ctcctttgcc atcccgatcg ggttctacgt cgaccgattg agcgctatca tgatgacgct
     8641 gatcaccggt gtcagcgtga tc
//
LOCUS       DATIFR010000039         4274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1361480, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000039 DATIFR010000000
VERSION     DATIFR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361480"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VLD60_02080"
     CDS             <1..499
                     /locus_tag="VLD60_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43766.1"
                     /translation="LTHEDRFGQTPNWLGEGLRRSMLNFLEGRGLTLDVRQWFDCPAP
                     KPQVASNWLKRLLKNRHQKDDPQIERRFVWLGNAPAYTGSGKRRRFMLPGKTSAPSFT
                     LKETEAQWLADLIEQSSPRLNRSAPYPLIRQAAATFPGSQKEFATFMASPAWSRIRSA
                     GLLLV"
     gene            complement(597..1232)
                     /locus_tag="VLD60_02085"
     CDS             complement(597..1232)
                     /locus_tag="VLD60_02085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSB43767.1"
                     /translation="MALRLGDEAPNFTAETTEGTINFHEWLGNGWGILFSHPKDYTPV
                     CTTELGTVAKITPEFKKRGVKVIAISVDPLDSHKGWISDINETQHTTMNYPIIADPDK
                     KVATLYDMIHPNALDNMTVRSVFIIGPDKKVKLTLTYPASCGRNFDELLRVIDSLQLT
                     SKFKVATPANWKDGEDCIITPAVNNDEAKTLFPKGFKIVKPYLRYTPQPNK"
     gene            complement(1325..>4274)
                     /locus_tag="VLD60_02090"
     CDS             complement(1325..>4274)
                     /locus_tag="VLD60_02090"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HSB43768.1"
                     /translation="FFRPVPLAVTALTCLILYTLVGFFLVPYVIKANLLPAVSEQLKR
                     PVMAKEVEFNPFALTLRLTGFEIQERDGAPLIGFEEFFINLQAVSVIRRAYVFDTIRF
                     TMPFVSVKVAKDGHVNLTDLVPPDESAAPPPASDLNKPKGEIPAVQIGHLEIAQGIVE
                     FRDESKPTPISIDIVPIGIVLKNFYTKPGGDNTYAFTAELGKGETLAWEGTISLEPIR
                     SEGKLALSGVKLSTLFQYIQDQFNFEIPSGTIQARGEYRFDAAGTPINLEVSNTSLHL
                     VDISLVEKGEPEPVITVPTLDIHGIQFDLRQRTISIARFSIADTTARAWRNPDGSLNL
                     QTLFAPVKTESNVPQPVSPPAKPAPTSAAGEQPWSVSLKDMRVTNHTVHVEDRSLGLP
                     MRADVTGLSVKTHDVAFPIKGPLPVEVELTLNESGRMKVEGQVTVEPFQTDVAIGLKD
                     IALQPFQPYLEQLARIAVDSGSIDLDGQLHLALKHPKTPLLTFHGNVGVKSLAIADRD
                     QGSPVASWRRLQFRQIALALDPTTVVIDEVGLEQPKVHLVVQPDGQLNLKSLLVQAEA
                     VPSQPAPEKPVPATKKAAPPTVAVRTVKLLKGMATFRDESIAPAVQAGLFDLTGTVKG
                     LSSKQLAKADVDLSGNIDRVAPLKVTGTINPLSENAFTDLMIKFDNVDLTAVAPYTGK
                     YVGYPINKGKLFLDLSYKVSQKQLEAENKVSIDQLTFGEKTDSPDATSLPVPFAVALL
                     KDRQGRIDIDLPIRGDLNDPDFKYGRVVLSTLMNLLARMVASPFTLVANLIPGGGNAE
                     DLQFVEFEPGSAAMLEGELKKCEALTKGLGERPGLRLEITGTADPSRDRKAIGLKRLN
                     AQLLAQWQQGKSAAKGADLPKAEEERLIKELFDEQHSRQSETQPTQKSDVAPKPPTID
                     EMREEILASIPVEEAALRTLAHERASETLAQLTGEGKLAADRVFLTEVDLTASDHEKV
                     RSRLNVTAGP"
BASE COUNT          901 a         1217 c         1142 g         1014 t
ORIGIN      
        1 tttgactcac gaggatcgct ttggtcaaac cccgaactgg ctcggtgaag gtctccgccg
       61 gtcgatgctg aattttctcg aagggcgggg cctgacgttg gatgtgcggc aatggttcga
      121 ttgtccggcg ccgaaacctc aagtggcatc aaattggttg aaacggttgc tcaagaacag
      181 acatcaaaaa gacgatccac agattgaacg acgattcgtg tggcttggca acgcaccggc
      241 ttacaccggt tcgggaaagc ggaggcgttt catgctgcct gggaaaacct ccgctccttc
      301 attcaccctg aaggaaacgg aagcacagtg gctggccgat ctcatcgagc agagtagccc
      361 ccggttaaac cgctctgctc cctacccgct cattcggcaa gctgccgcaa cgtttccggg
      421 atcccaaaag gagttcgcca catttatggc ttctccagcc tggtcaagga ttcgatcagc
      481 aggacttctc ctcgtttgag taatcggtgg gactctttgt gtgacttgcc ccgaaaacac
      541 gaagggcggg agggggctag cccctcccgc ccttcgtgtt ttctgattcc cgttccctac
      601 ttgtttggct gcggcgtgta gcgaagatag ggcttcacga tcttgaagcc tttcgggaat
      661 aatgtcttag cttcatcatt gttcacagcc ggggtaatga tgcaatcctc tccgtccttc
      721 caattggcag gggttgcaac tttgaacttc gacgtcagct gaagagaatc gatgactcta
      781 agcaattcgt cgaagtttcg accacatgaa gctggatagg tcaaggttaa ctttaccttc
      841 ttatctggcc cgatgatgaa gaccgagcgg accgtcatgt tatccagcgc gttgggatgg
      901 atcatatcgt agagcgtggc gaccttcttg tctggatcgg caatgatggg gtaattcatc
      961 gtcgtgtgct gcgtttcgtt aatgtcgctg atccagcctt tgtgagaatc caatgggtcc
     1021 acgctgatcg cgatcacctt gacaccccgc ttcttgaatt caggggtaat cttcgcgacg
     1081 gtacccaatt cagtcgtaca gaccggtgta tagtctttcg gatgcgagaa cagaattccc
     1141 cagccgttgc caagccactc gtggaaattg atcgtcccct ccgttgtctc cgccgtaaaa
     1201 tttggtgctt catctcccaa tcgcagtgcc ataatcgacc tccttgttaa ctcattcgtc
     1261 cggcctaccg aataatgcac tacgatactg tccttccaac accaagttca agtgggagcc
     1321 attcctacgg cccagccgtg acattcaatc gactgcgcac cttctcgtgg tccgatgcgg
     1381 tcaaatcgac ttccgtcaag aagacccggt ccgcagctaa cttgccttct ccggtaagtt
     1441 gggccagcgt ctcacttgct cgttcatggg ccagcgtacg gagggctgcc tcttcaaccg
     1501 gaatcgacgc caggatttcc tccctcatct catcaattgt cggtggtttc ggagcaacat
     1561 ccgacttctg cgtcggctgc gtttccgact gtcggctgtg ctgttcatcg aataattctt
     1621 tgatcaagcg ctcctcctcg gctttcggca gatcagcccc ctttgctgca ctcttccctt
     1681 gctgccactg ggcgagtagc tgtgcgttga gtctcttgag accgatcgcc ttgcgatctc
     1741 gactgggatc ggcggtgccg gtgatttcca atcgtaaacc gggccgttcc cccagccctt
     1801 tcgtcaatgc ctcgcatttc ttgagttctc cctcgagcat cgcagccgat cccggctcga
     1861 attcgacgaa ttgcaagtcc tcggcattac cgccaccggg aatcaggttg gccacgagtg
     1921 taaagggtga tgccaccatc ctggcgagaa gattcatgag cgtcgacagg accaccctcc
     1981 catacttgaa gtccggatcg ttgaggtccc cgcgaatggg caagtcgata tcgatcctcc
     2041 cctggcgatc tttcaataga gccaccgcaa acggaacagg caacgacgtg gcatccgggc
     2101 tatcggtctt ttccccgaac gtcagctggt cgatcgacac cttgttctcg gcttccagtt
     2161 gtttctgaga caccttgtag ctcaagtcga gaaagagttt gcctttattg atcggatagc
     2221 cgacgtactt gccggtatag ggtgcgacag ccgtcaaatc gacattgtcg aacttgatca
     2281 tgaggtccgt aaacgcattc tcgctcaacg ggttgatcgt gcccgtgacc ttcaacggag
     2341 ccaccctgtc tatgttgccg gataggtcga cgtcagcttt cgccagctgt ttcgacgata
     2401 gacccttcac cgttcccgtc agatcgaaca gtcctgcctg gacagccggt gcgatcgatt
     2461 cgtcccgaaa cgttgccatg cccttgagta acttcaccgt tctgacggcg acggtggggg
     2521 gcgcggcctt cttcgtcgct ggcaccggct tttccggtgc cggttgagat ggaacggctt
     2581 cggcctgcac caaaagactt ttgagattca gctgcccatc cggttgcacg accaggtgaa
     2641 ccttcggttg ttctaagccc acttcgtcaa tcaccaccgt ggtgggatcc agagccaagg
     2701 cgatctgacg aaattgcaac cgtcgccatg atgccaccgg cgagccttga tcccgatcgg
     2761 cgatcgccag cgatttgacc ccaacgttcc cgtgaaaggt gagcagcgga gtcttggggt
     2821 gcttcaacgc cagatggagc tgtccatcca gatcgatcga tcccgaatcc acggcgatac
     2881 gcgcgagctg ttcgagatat ggctgaaaag gctgaagcgc aatatccttt aatccgatgg
     2941 ccacatccgt ctgaaacggc tcgactgtga cctgtccctc cactttcatc ctgcccgatt
     3001 cgttcagagt cagttcgact tcgaccggga ggggcccttt gatcggaaag gccacgtcgt
     3061 gggttttgac cgacagaccg gtcacgtctg cgcgcatcgg cagcccgagc gaacgatctt
     3121 caacatgaac ggtatgattc gtgacacgca tgtctttgag cgagactgac cagggttgct
     3181 cgccggcggc cgacgttgga gccggctttg ccggtggcga aacaggctgc ggaacgttgg
     3241 attcggtctt gaccggagca aatagggttt gaagattgag tgagccatca ggatttcgcc
     3301 aggcgcgggc cgtggtatcg gcaatgctaa acctcgcaat cgagatggtc cgttgtcgaa
     3361 gatcaaattg aatcccgtgt atatcgagcg tcggcacggt gatcaccggc tccggctctc
     3421 ccttttcaac caagctgata tcgaccaaat gcagcgacgt gttggatacc tccaggttga
     3481 tcggagtgcc cgctgcatca aatcggtatt ccccccttgc ctggatcgtt ccggatggaa
     3541 tttcaaaatt gaactggtcc tggatatact gaaacagtgt cgaaagcttc acgcctgaga
     3601 gggcgagttt cccttctgag cggatcggct ccaacgagat tgtgccttcc caagccaagg
     3661 tctcaccctt ccccaactca gccgtaaagg cataggtgtt gtcgccaccc ggctttgtat
     3721 aaaagttctt cagcacgata ccgatcggga caatatcgat agaaatcggc gttggcttcg
     3781 attcgtcgcg aaattccacg ataccctgtg cgatctcaag atggccgatc tgcacggctg
     3841 ggatttcacc cttcggcttg ttgagatcac tcgccggtgg cggcgcggcc gactcgtccg
     3901 gcggaaccag atcggtcaaa ttcacatgac cgtctttcgc cacctttacc gatacgaacg
     3961 gcattgtgaa ccgaatggta tcgaagacgt aggcccgcct gatgaccgac accgcttgga
     4021 ggttgatgaa aaactcctcg aaaccgatca acggagcgcc atccctctcc tggatctcaa
     4081 acccggtcag ccgcaaggtc agggcaaagg ggttgaattc gacttccttc gccatcaccg
     4141 gccgtttgag ctgttccgag acagccggca gcagattcgc tttgatgaca tagggaacga
     4201 ggaagaatcc gacgagcgta taaagaatca ggcaggtcag tgctgtaaca gcaaggggaa
     4261 caggacggaa gaac
//
LOCUS       DATIFR010000040         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2127240, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000040 DATIFR010000000
VERSION     DATIFR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127240"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VLD60_02095"
     CDS             complement(<1..275)
                     /locus_tag="VLD60_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43769.1"
                     /translation="MDTTVLGAWVATGLTLFIFSFLYKDNPLFKLAEHLYVGVSVGYT
                     IVKAYDTVIVHLIVKPIVEQGEVTLLIPVAIGLLMLTRYVPKAAWMSR"
     gene            complement(467..1306)
                     /locus_tag="VLD60_02100"
     CDS             complement(467..1306)
                     /locus_tag="VLD60_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43770.1"
                     /translation="MTFAERMLRIDRRIIFVVIGVCTLLPLLYPVGLPIKISPEVRGV
                     YDYMEALPERSVFLLSLDFDPASKPELHPQAIALLRHAFRKNLRVIAMTLWVSGTGMA
                     DQLLTQVAKEMGKENGKDYVFLGWSPGGTAVIINMGQDLYTAFPSDYGGKPTKGLPVL
                     EGVLSLKDVNYAISLGAGNPGVEAWYVFGKDKYKFELGGGCTGVIAPGLYPLLRSGQI
                     NGLIGGLRGAAEYESLIGQKGRAVAGMDAQSATHMAIIVLVIICNLFYFSLRKVARQQ
                     GGG"
     gene            complement(1410..2060)
                     /locus_tag="VLD60_02105"
     CDS             complement(1410..2060)
                     /locus_tag="VLD60_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43771.1"
                     /translation="MIFLRRQVPLLITMITGLVFAAQYYIPHPASEQLLTSATKWLQI
                     IGGFALVLGVTSLFHQHAAKIRRKEAGWGYSLVLYAGMLVTIAVGLWANGKESIDGAM
                     TAFGWIYSFMMVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLIAAVIVMMGRVPL
                     GEYLIPISGDLSQWILNVLNASVRRAILIGVSLGAVALSFKIIFGVERSYLGGGKE"
     gene            complement(2057..>2500)
                     /locus_tag="VLD60_02110"
     CDS             complement(2057..>2500)
                     /locus_tag="VLD60_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HSB43772.1"
                     /translation="GKPDLFKEDSNFFITSDQFSYTAAVDGIMLRKKPAANFFMGSYF
                     AESLLLTETGASTGAIQIAGTDSDHQLPFFVTTCDYTLIGEELYAASAYLSREPIQIG
                     TLRGQDIGKAFILSVIAAGTALATLAVLTGAQWPHLFLDLFKDLK"
BASE COUNT          578 a          774 c          662 g          486 t
ORIGIN      
        1 cgagacatcc aggccgcttt cggcacatag cgggtcaaca tgagcaggcc gatcgcgacc
       61 ggaatcagga gcgtgacttc cccctgctct acaatcggct tgacgatcaa atgcacgatg
      121 acggtgtcgt aggctttcac aatggtgtac cccaccgata caccgacgta gagatgctcc
      181 gctagcttga agagcggatt gtccttatag aggaacgaga agatgaacag ggtcaaccct
      241 gtcgctaccc acgcgcctaa aacggttgtg tccattcgct agattatcct tgacacactg
      301 gagccaactt ccggtccccg acttaatacg cggccgggat gagtcctcca ctgcgcgcgt
      361 ccgacgaggg tctgcttttc gttctcaccc gcccaccctc gcggcgccaa gacgccgcgt
      421 tttcccaaca aaccgcgcgt tgcgcgagca cagaggactc accaggtcac ccaccaccct
      481 gctgcctcgc gactttccgc aatgaaaaat aaaagaggtt gcagataatc acgagcacga
      541 ttatcgccat atgcgtggcc gactgcgcgt ccatcccagc caccgctctc cccttttgtc
      601 cgatcagact ttcatattca gccgcccctc ggagcccgcc gatcagccca ttgatctgtc
      661 cgcttcgcag cagaggatag aggcctggcg caatcacgcc agtacagcct ccgcccaact
      721 cgaacttgta cttgtccttt ccgaacacat accaggcttc gacgcctggg ttcccggctc
      781 caagactgat cgcgtaattc acatccttca agctgagcac gccctccagc acaggcaagc
      841 ccttcgtcgg cttgccacca tagtcgctgg gaaaagccgt gtagaggtcc tggcccatgt
      901 tgatgatgac ggccgttccg cctggactcc agccgagaaa gacgtaatct ttcccgtttt
      961 ccttccccat ctccttagcc acctgggtca gcaactgatc ggccatgccg gtgccggaca
     1021 cccagagggt catcgcgatc acgcgcaggt tctttctgaa ggcatggcgc aacagggcga
     1081 tcgcttgcgg atgcaactcg ggtttcgagg ccggatcgaa gtcgagcgag agcagaaaca
     1141 ccgaccgttc cggaagcgct tccatatagt catagacacc acgcacctcc ggtgaaatct
     1201 tgatcggaag tccgaccgga tacagcaggg gcagaagcgt gcaaacgccg atcaccacga
     1261 agatgatccg cctatcaatg cgcaacattc gttcagcaaa cgtcatatga gaatccgtga
     1321 ggcgtcaggc gtgaggcgtc aggtgtgtga gcattacgga gctgcgcttc ctctccgatc
     1381 gcctcaccct tcacgcctta ccccttacgc tattctttcc ccccgcccag gtacgaccgt
     1441 tcgacgccga aaataatctt gaacgacaat gccacagccc ccagactcac gccgatgagg
     1501 attgcccgcc gaaccgaggc attgagcacg tttagaatcc attgcgagag atcgccgctg
     1561 atcggtatca gatactcgcc taacggcacg cggcccatca tgacaatcac ggcggcaatc
     1621 aacagcaccg cggcttcacg acttctggct ctgaatgacc gataggcggc cgaagcaatg
     1681 aagaaggcca agatggcgaa catggtgccc tgcagtggga ccatcatgaa ggaatagatc
     1741 cacccgaacg ccgtcatcgc gccatcgatg ctctccttgc cgttggccca gagtcccaca
     1801 gcaatcgtga ccagcatgcc ggcatacagc acgagactgt aaccccaccc ggcctctttt
     1861 cgcctaatct tggcggcatg ctgatgaaag aggctcgtga cgcccaatac cagcgcaaag
     1921 ccaccgataa tttgcagcca cttggtcgcc gatgtgagca gttgttccga agctggatgc
     1981 ggaatgtagt attgggccgc aaagaccaac ccggtgatca tcgtaatgag gagcggaact
     2041 tggcggcgga gaaagatcat ttgagatcct taaagagatc caggaagagg tggggccatt
     2101 gcgctccggt gagaaccgcg agggtcgcca gcgccgtgcc ggccgcgatt acgcttaaga
     2161 tgaacgcctt gccgatatcc tggccgcgca gcgtaccgat ctggatcggt tccctggaaa
     2221 gataggcact ggccgcatag agctcttccc cgatcaacgt gtagtcacag gtggtcacaa
     2281 agaacggcaa ttggtgatcc gaatcggtcc cggcgatttg aatggccccg gtgctggcgc
     2341 cggtctcagt cagcaggagc gactcggcaa agtaggaccc cataaagaag ttcgcagcgg
     2401 gcttcttgcg cagcataatg ccgtcgacag ccgcggtata actgaactga tccgaggtaa
     2461 taaaaaagtt cgagtcttcc ttgaagagat ccggcttacc
//
LOCUS       DATIFR010000041        11817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_788001, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000041 DATIFR010000000
VERSION     DATIFR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788001"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VLD60_02115"
     CDS             complement(<1..646)
                     /locus_tag="VLD60_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSB43773.1"
                     /translation="MSLNYVKFSPGFDAFMPKEYRDMVEHGPFGKKVTVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRIRFGDQPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVAMAKIAGCAYIATVVPNNPRRVE"
     gene            complement(815..2023)
                     /locus_tag="VLD60_02120"
     CDS             complement(815..2023)
                     /locus_tag="VLD60_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSB43774.1"
                     /translation="MDTTSVIGTKNKQGQVITNARVMMNEAPRTPSFFTGSEVIKEAV
                     RRANVDVMVAYPITPQSEAAALCGELFAEGYIKDYFRGESEFAVMSQCAGAAFGGARV
                     FTTTAGPGTMRAMENFPMWAGARLPIQCIVTCRGINSPLSIQPDTIEIAYLMNTGMLV
                     WHAETAQDFYDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDVVDLTPVDMCLPPYD
                     PYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEVWAAAERSRKHTIAWLD
                     GLIETENVDADILIVTSGTAVSQGREAIRLLADEGVRVGLVKIKTLRPWPEEEIREAT
                     KHASHIIVPEFNVIGWMAKEIKATIPHSERVVAGPRVCGGMTLPPEIIVEEIKKAIGM
                     RSGVLAGRGS"
     gene            complement(2085..2405)
                     /gene="erpA"
                     /locus_tag="VLD60_02125"
     CDS             complement(2085..2405)
                     /gene="erpA"
                     /locus_tag="VLD60_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSB43775.1"
                     /translation="MITITQTAEEKIRELMQEEQDTIGLRVYVKGGGCHGYQYGMAFE
                     SKMGDDDTVIEKGGVKVIMDSQSAPLLAGCEVDFQDTVQGSGFAIKNPQAKTTCGCGS
                     SFSA"
     gene            complement(2754..4226)
                     /locus_tag="VLD60_02130"
     CDS             complement(2754..4226)
                     /locus_tag="VLD60_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HSB43776.1"
                     /translation="MAHIKHSEQDIGVRSSVEREIKLAVADHFHLPHLSGTPLPRRQL
                     TSTYYDTAGYDLAHAGITLRYRVARGMHAWQLKLPLTHHRQELEIAGRQPVPPKTFCD
                     LLFLHIGQRPLMPIATLRTWRSGWHVLHDNVPIADVTLDNVAIVKNGAVIQRIREVEI
                     EQVNGEDRALKDIEQQLRRAGAETHDGRPKLFRALSLPASGPYGQPAPEAPVTEHVTW
                     ALTRHVRWLLAHDPGTRLGREPESLHQMRVAIRQLRAVLHAAKPLLVPHWADSLQKKL
                     AWLIQVLGPARDLDVQLAYFHDEAAKLDARDRTSLNRFIEYLESQREKAQEVLLHELR
                     RARYVHLVRRLREAAEKPSFIKSTLTVRDLAAREFKKLRKSIRRANTSPSNATVHKIR
                     IATKRARYIAELAEPAVGKPAMRFIRQSRAVQDVLGLHQDALQAEAHIRAFLKDSTNV
                     RAGFMAGRMVERQLERRKEAKSQMGPLWKALLKRGKQAWG"
     gene            complement(4204..4440)
                     /locus_tag="VLD60_02135"
     CDS             complement(4204..4440)
                     /locus_tag="VLD60_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSB43777.1"
                     /translation="MEKPLVAGKQPAILSLEPGAYYWRHCGRSKNQPFRDGSHKGTGV
                     TPVEFSISEKKQVARCQCKQTKTPPFCDGTHKTL"
     gene            4764..5033
                     /locus_tag="VLD60_02140"
     CDS             4764..5033
                     /locus_tag="VLD60_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43778.1"
                     /translation="MAGSIIPLRFNSRFRLSWSSWDSPLPAVLNRPPRVAIPGFDASP
                     PTLVWQTYNMQTKERGEIAKDGQSLGYSPASNTSSRPLWKISTAG"
     gene            5030..6085
                     /locus_tag="VLD60_02145"
     CDS             5030..6085
                     /locus_tag="VLD60_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSB43779.1"
                     /translation="MKDVILSGDVHFTCAQGGQVENKKFPLETQETKPVPDQENKVPI
                     RASLVYVVEFGKAGCKENWLFGGGELSLVGKAHNFLGGAVSRTLNVLLKKQPASRRGA
                     YMTGKRMVVVVLLMVFVTCPITVQAHDETEPETPMLTVTGTGQVAIAPDTAFVSFGME
                     TAGKSLTEAQRLNNQVMQKVTARLGELLIEKDRIQTSSFTVSPQYKPPPKRPTEAPPT
                     APEIIGYQVSNTVTVEVRNLEKVATVIEESLAAGANHFQGLHWALRDEQQAKLSALKQ
                     AAARAREKATALSEALRVKLVRLMSANEGGHVIRPMAKSSRSMMAMEAGGGEPPIFSG
                     EIKVEATVTLVYEIGRE"
     gene            6185..6334
                     /locus_tag="VLD60_02150"
     CDS             6185..6334
                     /locus_tag="VLD60_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43780.1"
                     /translation="MKQGYRLIIVDKDGVLVSEFQLTERDLEDPVAFVSAIRQSIESI
                     EEEEI"
     gene            complement(6948..7649)
                     /locus_tag="VLD60_02155"
     CDS             complement(6948..7649)
                     /locus_tag="VLD60_02155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB43781.1"
                     /translation="MARVAVKLMIGQLATAALTAVVGLGSLLVSPAFAQQGHQHERMP
                     NVMEYLDRLDRPERDQDQKPAQVVEALRLKPGMSVADLGSGSGYFTRRFVEAVTETGK
                     VYAIDVEPEALKYVEESLVHMHRPYTAEFILARPDNPKLPVESVDLIFICNTYHHLED
                     RTTYVRNMKSALKPGGRIAIVDFYHDARSGELGFPKKHLVPRETVVGEFNAAGYTLLK
                     EHTFLPKQYFIEFVP"
     gene            complement(7655..7960)
                     /locus_tag="VLD60_02160"
     CDS             complement(7655..7960)
                     /locus_tag="VLD60_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSB43782.1"
                     /translation="MPTYVSLVKFTQHGLQTMKEKGVERAELVKKHAQTLGGNLVQAY
                     YCLGAYDVVAIWEFPDNKTAMKAAVMNAAMGHIEITTMPAIGRDEWKTLLHETFGTK"
     gene            complement(8850..11027)
                     /locus_tag="VLD60_02165"
     CDS             complement(8850..11027)
                     /locus_tag="VLD60_02165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSB43783.1"
                     /translation="MAHATDLIERTGVSMKWLSMMASRAVTAFWQMRTLPTDYPNQFT
                     TYLVVIISGVLIFCIGWGACTVERRLIASAGHSLVQAASDSASKLELMISERTADVEM
                     LASSAIARGDNRQALTLYLYRLLEVYHAYQWVGVTDANGILIAATDRAQVSQDRSKSL
                     WFKQAAARNQVTIVDAGTSKDAKGRLSITITAPVRTYDGRFLGAVAAVVEIPYLLDQL
                     DRTTRVLQGVAWLEDSHIEYQLLDGKGDLVADSALRQEGRLNLRTMGLLSAQLVAISE
                     RGFVEEQHLRRNKPVITAYAQVNLPHAHPASRWGILIRIDRDSILTPIRTFLKKLMVV
                     TMLIVAPIGFLLIWLVKQLHRKWHQVKQESERASKAEAALGAKAEALHAMVRAAKRMA
                     STPQIDEMLHQVLDIARKTTRARFAVLGILDREKGTFARTFTIGLDETRVRAIEALPV
                     EGGILNCLTQREGVLRIDDLSTEILLGNDLPPYLPLTSFLGISIRCHGQMFGQLYLAE
                     KVSSEGTTIAFTDLDEQILLMLSAQTAISIENLQLLQDSKEQALRDSLTGLLNHSASV
                     DALNRELSRADREHEGLAVIMADIDHFKRINDTYGHRVGDVVLHEVAKRVQEAARRYD
                     LVGRVGGEEFLVLLPGCDEASASEFAERIRFAICGTPIDTQVGPLTVTVSIGATTWSN
                     DKPAIPHLLWETADQALYRVKENGRNGIAFVPLQAGEDCQTAA"
     gene            11470..>11817
                     /locus_tag="VLD60_02170"
     CDS             11470..>11817
                     /locus_tag="VLD60_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43784.1"
                     /translation="MAKQHLSPSVSKSVSPAPRVPESAVLKKTPAKSPQAVTAVEMGA
                     RQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNALDACEEAGILPDITVKLEVV
                     ADGGTVPPVSQATR"
BASE COUNT         2343 a         3291 c         3535 g         2648 t
ORIGIN      
        1 tttccacccg gcgcggattg ttcggcacaa ccgtggcaat gtaggcgcag ccggcgatct
       61 tggccatggc gaccatatcc atcttctcga acttcttgcc gagcggagcc atcttgagca
      121 ccgctcctct ggtcgtcatc ccgctttcct gcccgccggt gttgccgtat acctcgttat
      181 ccagcatgat cgtcgtgaac cgttctttgc ggaaccagga atggagtacc tgctggaatc
      241 cgatgtcggc ggttccgccg tcaccggcca tcacgacgac atccttgggc tggtctccga
      301 atcggatgcg caagccgcgc gacaagccac tggcgacgcc gttctggtcg ccgtagttgc
      361 cgtacacgaa gggaatcgct gcctgcgaaa tggccagccg gccgcatcca gctgtcccga
      421 ccgtgatggt gtcttcggga ttcggaaacg cgatcatggc cagccggatg aacagagtca
      481 tggcgcagcc ggcacacatg ggatgctcct caagaatttc tttaaagctg cccatctgcg
      541 agacggtcac tttcttcccg aaggggccgt gctccaccat atcccgatat tctttcggca
      601 tgaacgcgtc aaaccctggg ctgaacttca cataattgag actcattcga tacctcgctc
      661 tctgtcttcg ttgcggtgat agtgctgacg cctctggcga aacgtattgc ttcgcggcgt
      721 cctctgtgtc ctcggcggcg atcatggctc agcgcggctg agcgtcacgc tgttcccggt
      781 tgaggtcgta ccgccgttct gcctgtgggc acgttcaact tccacgtccg gccagcacgc
      841 cggatcgcat tccgatcgcc ttcttgattt cctccacgat gatttccggc ggcagggtca
      901 tgccgccgca gacgcgcggt ccggcaacga cccgctcgct gtgggggatc gtggctttga
      961 tttctttggc catccacccg atgacgttga attccggcac gatgatgtgc gaggcgtgtt
     1021 tggtcgcttc acgaatttct tcttccggcc atgggcgcag cgtcttgatc ttgacgagtc
     1081 cgacgcgcac cccttcatcg gccaggagac ggatggcttc acggccctgg gagacggcgg
     1141 ttcctgacgt gacgatgagg atgtcggcat cgacattctc cgtctcgatc aggccatcca
     1201 gccaggcgat cgtatgcttg cgcgaccgtt ccgcggcggc ccagacttcc tgctgccagc
     1261 tcgcgtgcgt ggcgtagctg atgtagttgc tcttcatgat gaacggatcg cgcatcatgc
     1321 ggacaggcgg gcattccatg tccatgcagg gtaccggcga ccggtacgga tcatagggcg
     1381 gcaggcacat atcgacggga gtcaggtcga cgacgtcttt cgtatgggtg acgaagaatc
     1441 catcgcaaca gagcgccaaa gggagatgca cgtccggctc ctccgacacc atatagccct
     1501 tgagaatcca atcatagaaa tcctgcgcgg tttctgcatg ccagaccagc atgccggtgt
     1561 tcatcaggta ggcgatctcg atcgtgtccg gctggatcga caggggtgaa ttgattccgc
     1621 ggcaggtcac gatacattgg atcggcaatc tggctcccgc ccacatcgga aagttttcca
     1681 tggcccgcat ggtgccaggc ccggcggtcg tcgtgaagac acgggcaccg ccgaaggcgg
     1741 cgcctgcaca ctgcgacatc acggcaaatt cgctctcacc gcggaagtag tcctttatat
     1801 acccttcagc gaacagttca ccgcacaggg cggccgcctc gctctggggg gtaatgggat
     1861 aagccaccat gacgtcgaca ttggcacgcc tcacggcttc tttaatgact tcgctgcctg
     1921 taaagaacga tggcgttcga ggggcctcgt tcatcatcac gcgcgcattg gtgatgactt
     1981 gtccctgttt attcttggtt ccgatgacgg atgtagtgtc catggctcca ttccctcccg
     2041 gtctaggggt gccggtcaga gagctggcgg cacgcccgat gacgttatgc gctgaatgaa
     2101 ctgccgcagc cgcaagtcgt cttcgcttgc ggattcttga tcgcaaagcc ggagccttgc
     2161 acggtatcct gaaaatcgac ttcgcagccg gccagcagcg gggcgctttg cgaatccatg
     2221 atgactttca caccaccctt ctcgatgacc gtgtcgtcgt cgcccatttt ggactcgaag
     2281 gccatcccgt actggtagcc atggcaaccg ccgcctttca catacacgcg cagaccaatc
     2341 gtgtcctgct cttcctgcat gagttcccga atcttttctt cagccgtctg tgtgatggtg
     2401 atcatgtcgt tgctccttgt tccgatggac tgacgtgggt ggcaccgcac gaccgactcg
     2461 catgtcgaga caccaatccc ggccggaggc ggtgaacgcc tcctcatcgg aggaatgcat
     2521 gacaagcggt tcggaaggca ctctggcgta gaagcggcgg agaccaggga atcagtcgcg
     2581 cgaggagagt tcgcagaagc gcgaggatag gacagtcggc gtggctctat acgtcataaa
     2641 atgagcctgg gcgcattagg cataggagtc aagcgctatt aaggggtagg tagacggaac
     2701 attagggatg gctccgttgc tgtgaggggt gtcgtggaag gcggccggtg tgcctatccc
     2761 caggcttgtt tgccccgttt caacagcgct ttccagagcg gtcccatttg gcttttcgct
     2821 tccttgcgcc gttccagctg gcgttcgacc atacgtcccg ccatgaaacc ggcccggaca
     2881 ttcgtcgagt ccttgaggaa cgcacgaata tgcgcttcgg cttgcagggc gtcttggtgc
     2941 agaccgagca cgtcttgcac tgcacgggac tgtctgatga agcgcatggc cggtttgccg
     3001 actgccggct cggcgagttc agcgatgtac cgtgcccgtt tggtggcgat tcggatcttg
     3061 tgcaccgtgg cattgctggg tgaggtgttc gcgcgacgga tcgatttgcg caattttttg
     3121 aactcccggg cagcgaggtc gcgtaccgta agtgtggact tgatgaagga cggtttttcc
     3181 gccgcttctc ggagacgcct gacgaggtga acatagcgcg cgcgcctcaa ctcatgcaga
     3241 agcacctcct gagctttctc tcgctgagat tccagatatt cgataaatcg gttcagcgac
     3301 gttcgatctc gtgcatccaa cttggccgct tcgtcgtgaa aataggcaag ttggacgtca
     3361 agatctcgcg ccggtccgag cacttggatg agccaggcca acttcttctg caacgagtcc
     3421 gcccaatgag gaacgagcag gggttttgcc gcatgcaaca cggcgcggag ttgtctgatg
     3481 gcgacgcgca tttgatgcag gctttccggt tcccgtccga gcctggtgcc gggatcatgc
     3541 gcgagcagcc accggacatg acgtgtgagc gcccatgtga cgtgctccgt gaccggcgct
     3601 tccggtgcag gctgaccata ggggcctgag gccggtaggg agagggcgcg aaacagcttg
     3661 gggcgtccgt catgcgtttc ggctcccgct cgacggagtt gctgttcaat atccttcagc
     3721 gcgcggtctt cgccattgac ttgttcaatc tcaacttcac gaatgcgttg tatcaccgcg
     3781 ccgttcttca cgatcgccac attatcgagc gtgacgtcgg cgatcggcac gttgtcatgg
     3841 aggacgtgcc atcccgaacg ccatgttcgc agggttgcga tgggcatgag cggccgctgc
     3901 ccgatatgca gaaagagcag atcacaaaat gtttttggcg gaacgggctg gcgaccggca
     3961 atctcaagtt cctgccggtg gtgagtcagc ggaagcttca gttgccacgc gtgcatcccg
     4021 cgcgcgactc gatagcggag ggtgatcccc gcatgagcca ggtcgtaacc ggcggtatcg
     4081 tagtaggtcg aggtcagctg ccgtcgtggt agcggagtgc cggacaaatg aggcagatgg
     4141 aagtgatcgg cgacggccag cttgatttcg cgctcaaccg atgatcgtac ccctatgtct
     4201 tgttcagagt gttttatgtg tgccatcgca gaagggtggc gtcttggtct gcttgcattg
     4261 gcagcgggcg acttgcttct tttccgatat ggaaaactcg accggcgtga ccccggttcc
     4321 tttatgggac ccgtcacgga agggttggtt cttggaacgc ccgcaatgac gccagtagta
     4381 ggctccaggt tccaacgaca gtatcgccgg ttgcttgcct gcaacgagag gtttctccat
     4441 gagtggttct tcttggtcgg ggtgatagat caattcattt gttcagcgac aggaccgctt
     4501 ccgaaacctg tttccggaat ggatccgttg ccgcgacgca gagcgcgatc tcggtgactc
     4561 cgatcacgaa cgtctcgagt catgttcgca gtgtgaggaa ttaaatcatc aaaggcaaga
     4621 cccgcttttt cgtcatcccg attcttgaca ctatgcaaca gacagcctag ccttgcggcg
     4681 aagtgccttg cgcgtgaacc agtggacgat cgttgatgct cctgtcaact ccgcggacta
     4741 tgcgaaggag ggcgtgacga tggatggcag ggtcgatcat tcccctccgg ttcaactcgc
     4801 gcttccggtt gtcctggtcg tcatgggatt ctcccctgcc ggctgtcctc aaccggcccc
     4861 caagggtggc cattcccggg tttgatgcct ctcctcccac cctggtctgg cagacgtaca
     4921 acatgcagac gaaggagcgg ggagagattg cgaaggacgg ccagtccctt gggtactctc
     4981 cggcgagcaa tacgtcgtca cgcccgctgt ggaagatctc aacagcgggg tgaaagatgt
     5041 catcctaagc ggcgacgtcc actttacttg cgcacaaggc ggccaggttg agaacaagaa
     5101 attcccgctc gaaacgcagg agaccaagcc ggttcccgac caggagaata aggtgcccat
     5161 cagagcctct ctggtatatg tggtggagtt cggcaaggcg ggctgtaaag aaaattggct
     5221 gttcggcgga ggagagcttt ctttggtggg gaaggcgcat aatttccttg gcggcgctgt
     5281 gagccggacg ttgaatgtcc tgttgaagaa gcagccggca agcagaagag gagcctacat
     5341 gacgggaaaa cgcatggtcg ttgtcgtact gctgatggtt tttgtgacgt gtccgatcac
     5401 ggtccaggct cacgatgaaa cggaaccgga aacgccgatg ttgacggtga ccggcacggg
     5461 ccaggtggca attgcgccgg atacggcatt cgtgtcgttc ggcatggaga cggccggcaa
     5521 gtcgctcacg gaggcccagc ggctgaacaa ccaggtgatg cagaaagtga cggcgaggct
     5581 gggggagttg ctgatcgaga aagatcgcat ccagacatcg tcctttaccg tatcgccgca
     5641 atacaaaccg ccgccaaaac gcccgaccga agcgccgccc acggcaccgg agatcatcgg
     5701 ctatcaggtg agcaacactg tgacggtcga agtgcgcaat ctggagaagg tagcgacggt
     5761 gatcgaggag tcgttggcgg cgggggcgaa tcactttcag gggctgcact gggccctgcg
     5821 cgacgaacag caggcgaagc tgagcgcgtt gaagcaggct gcagcgaggg cgcgtgagaa
     5881 ggcgacagcc ctgagtgagg cattgagggt caagctggtt cgactgatga gtgcgaatga
     5941 gggcggtcac gtcatccgtc cgatggccaa atcctcacgg tcaatgatgg cgatggaagc
     6001 cggcggcggg gagccgccga tcttttcagg tgagatcaaa gtagaggcga ccgtcacgtt
     6061 ggtctacgaa atcggacggg agtgattccg gggcgttttg gccggagatc gcgaatctga
     6121 gagcatgtgg agtaatggat cacccttccg gttctcgacc gatgacgttg agaaaggtct
     6181 cacagtgaaa caaggttatc ggctgattat cgtggataag gatggtgtcc tggtcagtga
     6241 gttccaactg accgagcgcg atctcgagga tccggtggcg ttcgtctcgg cgatcaggca
     6301 gtcgatcgag agtattgaag aggaagaaat ctagcgcagc gctttgtgga cgccgtccag
     6361 cccagacttg gcatatacgt cgccttccta tcgacctgcc ttacccccag gaatttcagc
     6421 caaaatgagc gctttttcat gaagaccctt agggcttctt tccacaggaa agccgattcg
     6481 ctgtgtataa ctgctctgtt tcagcgtcgc taatacgtat ttgcccgatt tgctgtcagg
     6541 gtgatgggca atgcttctcc ttgatagcag gaattgtgga aaaggaaaaa aatgagccgt
     6601 caagccacaa aatatagggg gtccatttaa gggggcacaa tgcctaaaat ttaggcaaac
     6661 aggtccgaga tctctgcttg tcggtctttt ctcacataaa agtcgtttca tgcacagagt
     6721 tatccacaga agctggggag tcaaaaaacg atgcattttg ggactgctga aattacatgg
     6781 agatgcgtca tttgtctttc gatccatcgc tttcgtgagg aagaggcatt gaattgatca
     6841 tcgacaattt tgtgcagcgc tcgtcgatgc gtgaaaatat ttttttcact ccgcattgcg
     6901 tgtgatgtgc gcatgcgagg gaaggcgcgc agaagaatgt gcgtttacta cggcacgaac
     6961 tcgatgaaat actgtttcgg caggaatgtg tgttctttga gcaacgtata accggctgcg
     7021 ttgaattctc cgacgacagt ctcgcgggga acgaggtgtt tcttgggaaa gccgagttcg
     7081 ccggatcggg cgtcgtgata gaaatcaacg attgcgatgc gaccacccgg tttcagcgct
     7141 gatttcatgt tgcgaacata ggtggtgcgg tcttccaagt ggtgataggt gttgcagatg
     7201 aagatgagat cgacggactc gaccggcagc ttgggattat ccggacgggc cagaatgaat
     7261 tcggctgtat agggccggtg catgtggacg agactctctt cgacatactt taaagcttcc
     7321 ggttccacat cgatggcata gaccttgccg gtttcggtca ctgcctccac gaaccgccgc
     7381 gtgaaatatc cagagcctga tcccagatca gccacgctca ttccaggctt cagtctgaga
     7441 gcttcgacga cctgcgcggg tttttgatcc tggtcgcgct cggggcgatc gagccgatcg
     7501 agatattcca tgacattcgg catacgctcg tgctgatgcc cttgttgggc aaaggcgggg
     7561 gacaccagga gcgatccaag gccgacgacg gcggtgaggg ccgccgtcgc cagctgtccg
     7621 atcatcaatt tcacggcgac tcgagccatg atgattattt tgtcccgaac gtttcgtgaa
     7681 ggagggtctt ccattcgtcc cggccgatgg caggcatggt cgtgatctcg atgtgtccca
     7741 tcgctgcgtt catcactgcg gccttcatgg cggtcttgtt gtccggaaac tcccaaatgg
     7801 cgacgacatc gtatgcgccc agacagtagt aggcctggac cagattgccg ccgagcgtct
     7861 gggcgtgctt cttgaccaac tcagcccgct cgacgccttt ctctttcatg gtttgaagac
     7921 catgttgggt gaatttcacg agactgacat aggtcggcat gagacacctc ctgggtgagg
     7981 gtggaggaga gaaaaagggt ttccgagaat tctaggcttc tcaggcggtc cgtcacaaga
     8041 gagaaatcag cgacgcacag tcacccttcg catcttgctt gactcaccgc ggtcgcaggc
     8101 ctatcgtaat tgtgtgggag gagttaacgc cccgagggaa tatggttcat gaggagatcg
     8161 agcgctgagg atggtgtgca ggaccagctt gtactgggaa gcctaaagtc ctcccgagat
     8221 ctccgccgta cctaatcgtc ctcggttagg tgcgtgctcc tcccacccta accccgcatc
     8281 gcctaccaac acttctgctg caagagtgga gtcgctgctg cgacaagcca cccggcgggc
     8341 gggctcggcg ctcagtccat cgacgtactg ctgtgagtac gcctcagtcc ttcgcacctc
     8401 cacgcgcccg tctcgccacg cgactccaca ctttcgcgac gaagtgcggc aggcgttgcg
     8461 gagtaacgtc cgttgaaaaa cctcgccggc cgcgttctcg tttcgcttcc atgctcgacg
     8521 tacaaggcaa gagtacgact cgcctgttcg ctcactgcgg cctttcggtg aacggcgcgt
     8581 ctcggcgccc tggggtgggc gggtgagaag atgagctttt tgaacggttg caacaatcca
     8641 gactttcttg cactcatccc cttggtttct gtagccttgc tggcactgtt tttgaccatc
     8701 ctggaaaact cggctacttg ccctgtggtg gcgttgcggg gcattcgata gttgaagatt
     8761 ggcgtcgctt ttggcctcgc tgaacaccca ttgtggactt ctcctctgag ttgatcgcgt
     8821 ctaagcgatg tttacgcgca ggtcgcctct caggcagcag tctggcaatc ctccccggct
     8881 tgtagcggca cgaatgcaat gccgttgcgg ccgttttctt tcacccggta cagggcctgg
     8941 tcggccgttt cccagagtag atgagggatg gcaggcttgt cgttcgacca ggtcgtggcg
     9001 ccgatgctga cggtgaccgt cagtgggccg acttgggtat cgatgggggt gccgcagatg
     9061 gcaaaccgga tgcgttcggc gaattccgat gccgaggctt cgtcgcatcc gggcaggagg
     9121 accagaaact cttcccctcc cacacgcccc accaaatcgt accggcgggc tgcctcctga
     9181 acgcgcttgg ccacctcgtg aaggacgacg tctcccaccc tgtgaccgta cgtatcgttg
     9241 atgcgcttga aatgatcgat gtcggccatg atgaccgcca ggccttcatg ctcgcggtcg
     9301 gctctggaga gttcgcggtt cagggcatcg accgaggccg agtggttcaa gaggccggtg
     9361 agagagtcac ggagggcctg ctctttggaa tcctggagta attgaaggtt ctcgattgaa
     9421 atggcggttt gtgccgacaa catcaacaga atttgttcat ccaggtcggt aaacgcgatc
     9481 gtggttccct cactcgagac cttctcggca agatagagtt gtccgaacat ctggccgtga
     9541 cagcggatcg agattccaag gaacgaggtg aggggtagat acggtggcag gtcatttccg
     9601 agcaagatct cggtgctgag gtcgtcgatg cggagcaccc cctctctctg cgtgaggcaa
     9661 ttcagtattc cgccttccac cggcagggcc tcgatggcgc gcacccgcgt ttcgtcgagt
     9721 ccgatcgtga acgtgcgagc gaatgttcct ttttccctat cgaggattcc caacaccgca
     9781 aaccgggctc tggtggtttt ccgcgctata tccaggacct ggtgcagcat ctcgtcgatc
     9841 tgcggtgtgg aggccatccg cttcgcagcg cgaaccatcg catggagcgc ctcggctttg
     9901 gcgccgagcg ccgcttcggc cttgctggcg cgctccgact cctgtttgac ttgatgccac
     9961 ttccgatgaa gttgtttgac cagccagatc agcaggaagc cgatgggcgc cacaatcagc
    10021 atcgtcacaa ccatgagttt tttcaggaac gttctgatcg gcgtcaggat gctgtcccgg
    10081 tcgatccgaa tcaggattcc ccatcgcgac gcagggtgcg catgcgggag gttgacctga
    10141 gcatacgcgg tgatgaccgg cttgttgcga cgtagatgct gttcctccac gaatccgcgc
    10201 tccgatatgg caaccaactg ggcggagagc agtcccatcg ttctcaaatt caggcgacct
    10261 tcctgtcgaa gcgcggaatc ggcgacgaga tcacccttac catccagcag ctgatattcg
    10321 atatgtgaat cctccagcca ggcgacaccc tgcaggactc gtgtggtccg gtccagctgg
    10381 tcgagaagat aggggatctc caccacagcg gccaccgctc caagaaaacg tccgtcgtac
    10441 gtccgtaccg gtgccgtgat cgtgatcgac aggcggcctt tggcatcctt gctggtcccg
    10501 gcatcgacaa tcgttacctg attccttgcg gcggcctgct tgaaccagag acttttgctt
    10561 cgatcctgcg acacctgggc ccgatcggtc gcggcgatca gtatgccgtt agcgtctgtg
    10621 acaccaaccc actgataggc gtggtagacc tcgagcaggc gatagagata gagggtcagt
    10681 gcctgtcgat tgtctcctcg cgcaatagcc gacgatgcga gcatttcgac gtcggcggtt
    10741 cgttccgata tcatcagctc caatttactg gcggagtcac tggcggcttg taccaggcta
    10801 tgccctgcgg aggctatcag ccggcgttcg accgtacagg ctccccatcc gatacaaaaa
    10861 atcagtacgc cggagatgat gacgacgaga tacgtggtga actgattcgg ataatctgtc
    10921 ggcaatgtcc tcatctgcca gaaagcggtt acggctcggg aggccatcat ggatagccat
    10981 ttcatggata caccggtgcg ctcgatgagg tctgtcgcat gtgccattcg ccacttcatg
    11041 atgctgcaat tcctccgttc agaaacgtac cctttctact acgtaggttt ctgcaaagag
    11101 aaggccaaag gcgccggaga attgcgatcg gggaaatagc agctatttcg agtgcgagcg
    11161 cttcagctcc atctttgcgc acagcaggaa aggaggttcc gcgacatctc tgatgacaat
    11221 ttgccggacc actattttgc acagtacaaa ggaggggtgc agacctatgc tttcgagttg
    11281 gccgatgtgt tctgcctgcg cggtcgatcc gatcatcgtg atgacacgtc accttctcgg
    11341 ttttcgcctg atctcgcgac aattttcccc tgcatactgc ggccgaaggc gtctcctcac
    11401 gaacgaggta gcatcggcgt tgctttttgt ggtaccaaga cacatcgcac ttaactgttg
    11461 agagggtgaa tggccaaaca gcacttgtcc ccatcggttt ccaaatctgt ttctcccgct
    11521 ccaagagttc ctgaatcagc cgttctcaag aagactcctg caaaatcacc gcaggcggtg
    11581 acggcggtcg aaatgggcgc gcggcagcgg gagatttccg tgtccgagtt ctttacgaag
    11641 aaccggcatc tgctcggctt cgacaatccc cgaaaagcgc tgctgacctg cgtgaaggag
    11701 gcggtcgata atgccctgga tgcctgcgaa gaggccggca ttctgccgga catcaccgtg
    11761 aagctggagg ttgtggcgga cggcggcacc gtgccgccgg tgagccaggc gacgcgc
//
LOCUS       DATIFR010000042         4988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_405291, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000042 DATIFR010000000
VERSION     DATIFR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405291"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VLD60_02175"
     CDS             <1..595
                     /locus_tag="VLD60_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB43785.1"
                     /translation="AHISTVGSVRMVREAKSRGIKVTAEACPHHFTLTEEVVRGFNTL
                     AKMNPPLRTWNDVQAIKEGLRDGTIDVIATDHAPHAAQEKQQGFTEAPFGIVGLETAL
                     PLTLALVEEGILTLEAAIEKLSSAPAKTFSLSKGTLAVGADADVAVIDPHVQWEVDPA
                     KFRSKSRNTPFAGWKVKGAVLTTIVGGRVVFEAPVVA"
     gene            592..1113
                     /locus_tag="VLD60_02180"
     CDS             592..1113
                     /locus_tag="VLD60_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSB43786.1"
                     /translation="MSRRTRWGLACALSCEFLALSLWVGGLIVLIGAVIPAVFNTFGG
                     QDSGGIFLTRSFEGYNRLVMGAIAMLALAMIYRWWSGEPLAQVSRGEVVVLAVMVLIA
                     GVIIAVLHPQAAALQAEAFAAKDEQARKTAFEAFFRLHMPIRSLYMVNLVLGIVLFVM
                     KAKRSLGREGVPA"
     gene            1110..2282
                     /gene="carA"
                     /locus_tag="VLD60_02185"
     CDS             1110..2282
                     /gene="carA"
                     /locus_tag="VLD60_02185"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSB43787.1"
                     /translation="MKKAFLALADGAVFEGRALGYEGETTGEVVFNTAMTGYQEVLTD
                     PSYKGQIITMTCPHIGNYGVTPDDIESRRIWAEGFVVKEASQLASNWRSRQHLHEYLR
                     EAKVVGIEGLDTRALTRHLRESGSQQGLISHVEGDVRRAVEKARRAPSIIGRDLAAEV
                     TCRESYQWANGSGEWAPASDGRGAAINRRWKVAAYDFGVKLNILRRLVDVGCDVTVVP
                     ASTTAEQVEALHPDGIFLSNGPGDPEGVPYAMEAVRRLIGRHPIFGICLGHQILGLAL
                     GLKTYKLKFGHHGANHPVMDLRTRKVEITSQNHNFAVQVPGPITTVPDLPPLVESTFG
                     KVAVTHVSLNDHSIEGLACMDQPVFSVQYHPEASPGPHDSAYLFGEFVQLMEKMHG"
     gene            2275..3243
                     /gene="sppA"
                     /locus_tag="VLD60_02190"
     CDS             2275..3243
                     /gene="sppA"
                     /locus_tag="VLD60_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSB43788.1"
                     /translation="MAKIQRLLLVLLLLGASGMQQGCITINLLEPAGPVQEVHLTGTG
                     DGKVLLLDLSGIISSQDKDALIQQPNMVAALKEELTKATKDELVKAVVLRINSPGGTV
                     NASDILYHEIKSFKASRKIPVVVSMMDVAASGGYYAAMASDAILVHPSTVTGSIGVIM
                     LTVNAKGLMEKVGVEANAITSGPRKDMGSPFRTMTAEEKAIFQGVIDSFYHRFLAVVQ
                     EGRPNLSADQIKKLADGRIYSGDQAKAAGLVDEIGYLDEAIELAKKKAGLTEARVVTY
                     RRHGEYQNNIYSRLLGTGSAFNSLAQLDLLSVVRGGSPQFMYLWMP"
     gene            3260..4234
                     /locus_tag="VLD60_02195"
     CDS             3260..4234
                     /locus_tag="VLD60_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB43789.1"
                     /translation="MADNRLSRAGDAVEQVLASSLSRRAALVLGARGVLGICSVLGAG
                     AALGLFSSLTGCYRAPGTARDQLIFFSEEKELQFGLSAYREVLRQARLSDNPEINEQI
                     QRVGKRIAEVANKPEYQWEFAVIEDEKMVNAFCLPGGKVAVFTGILKHTKDDVGLATV
                     MGHEIAHALQRHGVERMSRSILDQIAQLGALGAAASGHSSGAAIQGLLGAYGVNVSLP
                     FNRKQESEADFIGLRLMAQAGYDPRGAVPFWERMSGCPRQMIDKLCFRSQHAIPEFLS
                     THPSDLTRIKQIEAWLPDMLPYYHGPGNVPSPSPAPYRPAIGPLPQTG"
     gene            4241..>4988
                     /gene="carB"
                     /locus_tag="VLD60_02200"
     CDS             4241..>4988
                     /gene="carB"
                     /locus_tag="VLD60_02200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HSB43790.1"
                     /translation="MPKRTDIHTILLIGSGPIVIGQACEFDYSGTQACKALKEEGYRV
                     VLINSNPATIMTDPELADRTYVEPITLDVVEKVIERERPDALLPTMGGQTALNATMGL
                     VKRGTLEKFGVRLIGASAEAIHKAEDRDAFKQAMQRIGLRVPESGTAHSREEAVRVLD
                     RVGFPAIIRPSFTMGGTGGNIAYNREEFEKLIEWALAMSPVSQVLIEQSLIGWKEYEL
                     EVMRDLKDNVVIICPIENLDPMGVHTGDSIT"
BASE COUNT         1058 a         1277 c         1591 g         1062 t
ORIGIN      
        1 tgcccatatc agtacggtcg ggtcggtgcg tatggtgcgt gaagcgaagt cgcggggcat
       61 caaggtcacg gccgaagcct gtccccatca ctttacgttg acggaggaag tggttcgagg
      121 attcaatacc ctcgcaaaaa tgaatccgcc gcttcggacg tggaacgacg tgcaggcgat
      181 caaagaaggg ttgcgcgacg gcacgatcga cgtcattgcg accgatcatg ccccccatgc
      241 cgcgcaagag aagcagcagg ggttcaccga agctccattc ggcatcgtgg gtcttgaaac
      301 ggcacttccg ttaaccttag cgctggtcga agaagggatt ctcacgctgg aagcagccat
      361 cgagaaactc agcagtgcgc cggcgaagac ctttagcctc tcgaagggaa cgttggcagt
      421 cggtgccgac gcggatgtag ccgtcatcga ccctcatgtg caatgggagg tggatccggc
      481 caagtttcgc tcgaagagcc gcaatacgcc gtttgccgga tggaaagtga agggcgcggt
      541 tctgacgacc atcgtcggcg gtcgggtggt gtttgaggcg ccggtggtgg cgtgagccgc
      601 cgcacccgtt ggggacttgc ttgcgccctc agttgtgagt ttctggccct cagcctgtgg
      661 gtcggtgggc tcattgtgct gatcggtgcc gtgatccccg cagtgttcaa tacatttggc
      721 ggacaggatt ccggagggat ttttctgact cgatcgttcg aaggatacaa tcgtctcgtg
      781 atgggtgcca tcgcgatgct ggccctcgcg atgatctatc gatggtggag cggggagcct
      841 ctcgcacagg tcagtcgggg agaagtggtc gtgctggcgg tcatggtgct cattgccggg
      901 gtaattatag cggtgctgca cccccaagct gcagcattgc aagccgaagc cttcgccgca
      961 aaagatgaac aggcaagaaa gacagcgttt gaggctttct ttcggcttca catgccgatc
     1021 cgttcgctct atatggtgaa cctggtgctg gggatcgtgt tgttcgtgat gaaggccaag
     1081 cgatcgttgg ggagagaggg agtgccggcg tgaagaaagc attcttagca ttggctgatg
     1141 gggcggtctt tgaaggccgc gcgctcggct acgagggcga gacaaccggc gaggtcgtct
     1201 ttaataccgc catgaccggc taccaggagg tgttgaccga tccttcttat aagggccaga
     1261 tcattaccat gacctgtccg cacatcggaa attacggtgt gacgccggat gacatcgagt
     1321 cgcgtcggat ttgggcggaa gggttcgtcg tcaaagaagc cagccaactc gcgagcaatt
     1381 ggcgcagccg gcaacacctt cacgaatatt tgcgggaagc aaaggtcgta gggattgaag
     1441 ggcttgatac cagagcgctg acacggcacc tgcgtgaatc tgggtcgcag caggggctca
     1501 tcagccacgt tgagggtgat gtccggcggg ccgtcgagaa agccaggcgg gcccctagca
     1561 ttatcgggcg ggacttagct gctgaagtca catgccggga gagttatcaa tgggcgaacg
     1621 ggtccggcga gtgggctcct gcatcggacg gaagaggcgc ggccatcaat cggcggtgga
     1681 aggtggcggc gtacgatttt ggtgtgaaac tcaacatcct tcggcgtctc gtggatgtcg
     1741 gatgcgatgt gacggttgtg ccggcttcga cgacagcgga acaagttgaa gcgcttcatc
     1801 cggacggcat cttcctttcg aatggaccgg gcgatcccga gggggtgccc tatgcgatgg
     1861 aagccgttcg tcgactgatc gggcgccacc ccattttcgg gatctgtctg ggacatcaga
     1921 ttcttgggtt ggcgttgggg ctcaaaacgt acaagttgaa gttcggtcac catggggcca
     1981 atcatcccgt gatggaccta cgcacgcgaa aggtcgagat tacgtcgcag aaccataatt
     2041 ttgccgtgca agtgccgggt ccgatcacga ctgtcccgga tctgcctccc cttgtcgaga
     2101 gcacattcgg gaaggtcgcc gtgacgcatg tgagcttgaa tgatcactcg atcgagggac
     2161 tggcctgtat ggatcagccg gtgttttcgg tacaatacca tccggaggcc tcaccgggac
     2221 cccatgactc agcgtacttg tttggggagt ttgtccagct tatggagaaa atgcatggct
     2281 aagattcaac ggttgcttct cgtgctcttg ctgctcggcg caagcggcat gcagcagggc
     2341 tgtattacga tcaaccttct ggagccggct ggcccggtgc aggaggtgca cttgactggt
     2401 accggagacg gcaaagtctt gctgctcgat ctgtcgggca tcatcagctc acaagacaag
     2461 gatgcgctga tccagcagcc caatatggtg gcggcgctca aggaagagct gaccaaggca
     2521 acgaaggacg agctggtcaa agcggtcgtc ctccgcatca acagccctgg tggtaccgtc
     2581 aacgcatcgg acattctcta tcacgagatc aagtccttca aagccagtcg gaaaattccg
     2641 gtggtcgtgt ccatgatgga tgtggcggcc tccggaggct attatgcggc aatggcatcg
     2701 gatgccatcc tcgtccatcc ctcgaccgtg acaggcagca tcggtgtgat tatgctgacg
     2761 gtcaatgcga agggactcat ggagaaagta ggagtagagg caaacgccat tacctcaggc
     2821 ccgcgcaagg atatgggctc tccttttcga acgatgacgg cggaagaaaa agcgattttt
     2881 caaggtgtga tcgactcctt ctatcaccgc tttctggctg tcgtgcaaga agggcgtccg
     2941 aatttatccg ccgaccagat caagaagctc gcggacgggc gtatatattc aggggaccag
     3001 gccaaggccg ccgggctcgt cgatgaaatc ggctatctcg atgaggccat cgaattggca
     3061 aagaaaaaag ccggtctgac ggaagcgcgg gtcgtgacgt atcggcggca cggggaatat
     3121 cagaataata tttattcgcg gctgctcggg accggatccg ccttcaacag tctggcccag
     3181 ttggatttac tgtccgtggt ccgcggagga agtccgcagt ttatgtatct ctggatgccg
     3241 taggcaagac atgtctggta tggcagataa tcgattatct cgcgctgggg atgccgtcga
     3301 gcaggtgctg gcgtcatctc tcagccgtcg cgcggccttg gtattgggcg cgcgtggcgt
     3361 cttgggaatc tgtagcgtgt taggtgccgg tgctgcgctg ggactcttca gcagcctcac
     3421 aggctgctat agggcgcccg ggacggcacg tgatcagttg atttttttct cagaggaaaa
     3481 agaactgcaa tttgggctca gcgcctaccg cgaagtgcta cgacaggctc gtttaagcga
     3541 caatccggag atcaacgagc agatccagcg tgtcgggaag aggattgccg aagttgcgaa
     3601 caagccggag tatcagtggg agtttgcggt gatcgaggat gagaaaatgg tcaacgcgtt
     3661 ctgtttgccg ggaggaaaag tggccgtctt taccggcata ttgaagcaca ccaaggatga
     3721 tgtcgggctt gccaccgtga tgggtcatga aattgcgcat gcgctccaac gtcacggagt
     3781 ggagcgaatg agccggagta ttttggatca gattgcgcaa cttggcgcgt taggggcggc
     3841 agcttccggt cattcgagcg gagcggctat ccaaggtctg ttgggggcat acggggtgaa
     3901 cgtgtcgttg cccttcaatc ggaaacagga atctgaggcc gattttattg gattacgact
     3961 gatggctcag gctggctatg atccccgcgg agccgtgccg ttttgggagc gaatgagcgg
     4021 gtgtcccagg cagatgatcg ataaactctg cttccgttcg cagcatgcga tcccggaatt
     4081 tctctcgacc catccctccg acctgacgcg tatcaagcag atcgaagcct ggttaccgga
     4141 catgttgccg tactatcatg ggcccgggaa tgttccttct ccgtcaccgg ctccctatcg
     4201 gcctgcgatc gggcccttgc ctcaaacagg ctaaccagtc atgccaaaac gcaccgacat
     4261 ccacaccata ttattgatcg gttccggccc catcgtcatc ggtcaggcct gcgaattcga
     4321 ttactccggg acccaggcct gtaaggcgct gaaggaagaa ggctatcggg tggtgctgat
     4381 caatagtaat cctgcgacca tcatgaccga tcccgaactg gccgatcgaa catatgtgga
     4441 acccattacg ctggatgtgg tggagaaagt catcgagcgg gaacgtccgg atgcgttgct
     4501 cccgaccatg ggaggccaga cggcgttgaa tgcgaccatg gggttggtga agcgcggaac
     4561 gctcgaaaag tttggtgtga gattgatcgg ggcttccgca gaggcgattc ataaggctga
     4621 agaccgcgac gccttcaagc aggccatgca gcgcatcgga ctgcgggttc cggagagcgg
     4681 cacggctcac agccgtgaag aggcggttcg cgtgctcgac cgcgtgggat ttcccgccat
     4741 cattcgcccg tccttcacga tgggagggac cggcgggaac attgcctaca atcgtgaaga
     4801 gtttgagaag ctgatcgagt gggcgctggc gatgagtccg gtaagccagg tgcttatcga
     4861 gcaatccctg atcgggtgga aagaatacga acttgaagtg atgcgggatc tgaaggacaa
     4921 cgtcgtcatc atctgcccga tcgaaaatct cgatccgatg ggggtccaca ccggcgacag
     4981 tattactg
//
LOCUS       DATIFR010000043        10922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1554567, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000043 DATIFR010000000
VERSION     DATIFR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554567"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1261
                     /locus_tag="VLD60_02205"
     CDS             107..1261
                     /locus_tag="VLD60_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB43791.1"
                     /translation="MLKQNRIVLAIAGAVFLVIVILETVAPANVVGAYGFVLPILLVA
                     TLRSRPLMIATVVACVAATYLGLMQPTKPGRFQSAVINRTVVAGVLLMVAYIGMTREE
                     RKAREEAARAALAQQTESLLRANTLLVDAKDQLNRSERLAAVGQLVASVAHEVGTPLH
                     SIAWHVQALAEEPGVTPEMKKRITVIDEQLNRVVRIIQDLLSSTRQRKPEPIWMSVER
                     VISPATVLMEPAFHAKGVSLTTELAIGLPMVWADAEKIHQVLVNLLTNALVATPQHGV
                     VVVRVGGRPARPEELEIGRRLAPDMSSILVTVSVQDTGIGMPQADVQKAFQPFFTTKE
                     VGKGTGLGLFLSRETVLAHGGALSLESEQGRGTTVTVTLPGKFAEPEGTV"
     gene            1544..2953
                     /locus_tag="VLD60_02210"
     CDS             1544..2953
                     /locus_tag="VLD60_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB43792.1"
                     /translation="MQPAKILVVDDDAVARELLAEALKKEGYDVEAFSSGEEAIARGR
                     EGRVDLVLTDIRMGAVDGLTVLREFKRMSADTAIVVLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKKEEIKLVVQRSLDHCRLVRENSKFREELKHKDDWSPLVGSSPAMLEVYKLVAR
                     VSEGKSTVLLQGESGTGKELIARAIHANSPRRDRPFVPVNCGALPDTLLESEMFGYEK
                     GAFTGAVGTKTGLFESANGGTLFLDEIGELGQALQVKLLRVMQDHEVRRVGGTGSIKV
                     DVRIIAATNRDLEQFVKEGKFRDDLFYRLNVVRITLPSLKERQEDIPMLAHHFLQKCG
                     AGATRAVRGFHPDTLALLKQYRWPGNVRELENAIERAVSLSHGPLLTPEDLPAAVHQA
                     SGAAEQKVEMAETGDEILLTLEEVEKRHLIRVLKEMKGNKVKAAKILGIDRRTLYRMA
                     ERFGLDLGEDAEGGDKDAG"
     gene            complement(3009..3455)
                     /locus_tag="VLD60_02215"
     CDS             complement(3009..3455)
                     /locus_tag="VLD60_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43793.1"
                     /translation="MNSLPCRCLLAFLFVTTTACAGPPLAHEPDIVDVTLPAPVETVR
                     AAVIQVLTDGGYDVDRRDDETLSTGYREEISGPWDWLLRWRFGTGRSRVEARVTSAAE
                     QSTRLWLNVLYEGKDGIFTNWEDSPTALPQSAENQLRLIKNALQIL"
     gene            complement(3452..3838)
                     /locus_tag="VLD60_02220"
     CDS             complement(3452..3838)
                     /locus_tag="VLD60_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSB43794.1"
                     /translation="MKKAKASGTSATLTGTPGIRYVGNEPAIPAVPCTIHAPVDGGVV
                     MADRAYVLIHVLPGQTSHVVRALGEIKQIKTIDPCWGKPDIITVVEVPDQDALTQLVL
                     SRIHAIEGVAQTDTHLVYRLKDTKAK"
     gene            complement(3847..5226)
                     /locus_tag="VLD60_02225"
     CDS             complement(3847..5226)
                     /locus_tag="VLD60_02225"
                     /inference="COORDINATES: protein motif:HMM:NF037406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43795.1"
                     /translation="MARAKTERSRSKTSAIEDLTQSIEQLQSYLPKVEDLGREGFPYI
                     EGARARTELQLRECIKRAFGEKSPEYQAHRHHKLAMGTPAETKQTVSLVKMLIATLEE
                     KKLELQGLKPSAPEKPSATSSPTPQGRPQMTLVPPTASTAQITMTSAAPATPPPITMS
                     VPLTTNVDMGATSTASPPSPTVSAPASITPPPSTEFTASQQVAAPTPKPSQITPDQVS
                     TLFRPNDTASVAPPIPQTQRSVSAPPPAPVSTPVSEPALPASDPSTIQPSISSPQHSA
                     TQEVPLASQPAQVRSVPRPATAVTSDSSLQAVDHLELCRKLCWRFHAIARQLRLRGEY
                     RATVAVEDEVDVQDLLHALLRVQFDDIGTDEWAPSYTNGAPRTTFLLDRDRLAIVVKK
                     TRTGLSTKDLMDQLRVDIERYRAQERCTTLFCFVYDPEGRIGNPRGLESDLATISDQF
                     TVDVLVAPK"
     gene            complement(5342..5587)
                     /locus_tag="VLD60_02230"
     CDS             complement(5342..5587)
                     /locus_tag="VLD60_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43796.1"
                     /translation="MTCRRCQGCMARDHFLDLQESGGEWWMEGWRCINCGHVFDPVVE
                     QNRRLHAAAMASAATAQQAQPVIAVSAQQELPLDIAA"
     gene            complement(5696..6040)
                     /locus_tag="VLD60_02235"
     CDS             complement(5696..6040)
                     /locus_tag="VLD60_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB43797.1"
                     /translation="MSKRSEWKIRTKPACLVAVKYIAKEFCGTGDLLDLSEEGLKIQG
                     SQGVHTGVQVALQITAADSAISIHIARAHVRWTKGGMFGVKFDTLEPAVKTQLLAFLA
                     TLAAPALTPKTV"
     gene            7033..7197
                     /locus_tag="VLD60_02240"
     CDS             7033..7197
                     /locus_tag="VLD60_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43798.1"
                     /translation="MAKAAVNRLDAEFFDVIGDGSKSPEMVAVGEVPWTGSILQLPEV
                     AEKIGAPDTN"
     gene            complement(7176..7252)
                     /locus_tag="VLD60_02245"
     tRNA            complement(7176..7252)
                     /locus_tag="VLD60_02245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7216..7218),aa:Arg,seq:cct)
     gene            complement(7489..8865)
                     /locus_tag="VLD60_02250"
     CDS             complement(7489..8865)
                     /locus_tag="VLD60_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSB43799.1"
                     /translation="MRYWSTLLMALVLAGLGLYLYIVEFPAKETEERQATAQKKVLLL
                     DQASITGLSFKTDRGDLVFERTQDKGWMLTAPLRTEADQREVQNLVRALVTGSVRRVV
                     EENPTTLSPFGLDKPVTTVTITAPSGQETLSIGDSGPLSSTLYVLRESDHALLLTDLA
                     AKDFVNKTLMTFRRKDILRVARSEIERIRLTYPTTEIVLYHLDEKPKGKWKIRYPIEA
                     DADLNEVRMLLFRLEDLKALGIVDPGPERDALARTLTTPKVRITLHAADGDRTVKLYQ
                     SDTSSGEAIAETGPEGPLYRINPTAIKDLTKELFTFQDKRLLGVDYTDIAMLSVTTRA
                     EHFVLINQNREWVLEDRPTEKLNQQVTDLFVSRVANVPAEERVVKQAGPLAPYGLVSP
                     AAEFVATGRDGKLAGKLAVGNAANGLAYAMGLRLQGIFQIRADLLTQIPAKKDLLASS
                     KNDDNTKP"
     gene            complement(8869..10392)
                     /locus_tag="VLD60_02255"
     CDS             complement(8869..10392)
                     /locus_tag="VLD60_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HSB43800.1"
                     /translation="MNLHSLPLGVIGIALAVAGMVGYSLAPDKLWLVTLAEGLALLCL
                     ILFFVLHFQTVKAFSTRRATRMGANSLLMVLLFVCILVIVNFLASRHSVRWDFSENQN
                     FSLSPQTHRIIRSLPRDVKITVFTREKDPGYQSYKERLDSYRQASPKVTVEFVDPERQ
                     PKIAQAYGISRTDTAIFESGELQARVTTPSEAELTGGLIRVSKSSKKRVLFLEGHGER
                     SLDDRERTGFFVAREALIKQGYDVGTLSLLKDTAVPDNTAILVLAGPQRSVTTEEQDR
                     IYTYVQQGGHLLLLIDPNTQADLNPLLKRWGLTAGPGVLVDLQDRLAQGDLTALLVRT
                     FTEHEITQDLTAAVLFPLSRHLTFDEQVGKDWDFVPLARTSPNSWAETDLRGRVVNLD
                     EKEDVKGPLPMAAALSPKSPLAEGKPRPAIVVIGNSTFATNAFLNFPGNSDFFLHTAG
                     WLAEERDLIAIGPKEEALRPFTPNPLQERALLYLQVVLLPAFTVIAGFMVWRKRRRL"
     gene            complement(10389..>10922)
                     /locus_tag="VLD60_02260"
     CDS             complement(10389..>10922)
                     /locus_tag="VLD60_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSB43801.1"
                     /translation="IILPMLTMRLFAEERKLRTFEFLMTSPVGLNEIVAGKFMSVLLV
                     FLGLLGLTGLIPLVLSLFSDFDWYPVLTGYLGLALLGALFLSVGVLASALTENQIVAA
                     FTSFGLLLILWLLAGVGSLLGDTTAGQAISYLSFMEHYDHLVRGLIDTKDLVYFLSAL
                     VLMLFLTHRVVDSARWR"
BASE COUNT         2313 a         2824 c         3409 g         2376 t
ORIGIN      
        1 acggttgcgt gaactcggtg cctgcttgcg ttcgacccgc ccgatcagtt aagacccttt
       61 tgaggatccg agggttgtcg gtgcatttcc tcgtctcata tcttctatgc tgaaacaaaa
      121 ccggatcgtt ctagcgattg cgggagccgt tttcctcgtc atcgtgattt tggaaaccgt
      181 ggcgccggca aacgtcgtcg gggcctatgg atttgtgttg ccgattctct tggtggccac
      241 gttgcggagc cgcccgctca tgattgcgac ggtcgtggct tgtgtcgccg cgacctacct
      301 cggactcatg cagccgacaa agccggggcg gttccaatcc gcagttatca accgaacggt
      361 cgtggccgga gtcctcctca tggtggcata tatcggcatg acgagggagg agcgcaaagc
      421 gagggaagag gcggcacggg cggcgttggc gcaacaaaca gaaagcctgt tgcgggcgaa
      481 cacgctgctg gtcgatgcca aggatcagct caaccggtcg gaacggctgg ctgccgtcgg
      541 gcagttggtg gcctcggtgg cgcatgaagt ggggacaccg ctgcattcga tcgcctggca
      601 tgtgcaggcc ctggcggagg aaccgggggt caccccggag atgaaaaagc ggataacggt
      661 catcgacgaa cagctcaatc gcgtcgttcg tatcatccag gatctgcttt catccaccag
      721 gcaacgaaag cccgaaccga tctggatgtc ggtggagcgt gtcatcagtc ccgccaccgt
      781 gctgatggaa ccggcgttcc atgccaaagg cgtttcctta acaaccgaat tggccattgg
      841 gcttccaatg gtctgggccg atgccgagaa gattcaccag gtcttagtga atcttctgac
      901 gaatgcgctg gtcgcgacgc cgcaacatgg agtcgtggtg gtgcgtgtgg gcgggcgtcc
      961 tgctcgaccg gaagagctcg agatcggacg gcgtttggca cctgatatgt cgtccatatt
     1021 ggtgacggtg tcggtgcaag acacgggaat cgggatgccg caggcggatg tgcagaaggc
     1081 gttccaaccg ttcttcacaa cgaaggaggt tggcaagggg accggcttag gactattctt
     1141 aagtcgggaa acagtgttgg cacatggcgg ggcgctgtcg ctcgagagcg agcagggacg
     1201 ggggacgacc gtgacagtga cattgcctgg caagtttgcc gaaccggaag gtacggtcta
     1261 gaaggatgtt gaaaaacccc gccagctttg tgctcgcctc gttcagagac tcaacgtacg
     1321 gagaagccag cttgaggcaa aataactatc cgctcgcatg tgatcgaatc gagcggccca
     1381 agcgaagctt ggtatgtacc tcctcgcctc ttcgctcgcg gcggcctcgc tggacggtct
     1441 ttttgaacat cctgtgggat ccgttgatcc cgttcctcgc atccggcagt cctgtcgctc
     1501 cagattccca gggcagtgct tcaataatct gctaaaggat acgatgcaac cagcgaaaat
     1561 tctcgtggtc gatgacgatg ccgtggcgcg tgagctgttg gccgaggcgc tgaagaagga
     1621 aggttatgac gtcgaggcgt tttccagcgg cgaagaggcc attgcgcggg gccgcgaagg
     1681 ccgggtggat cttgtgctga cggacattcg catgggggct gtcgatggat tgacggtgct
     1741 tcgcgagttc aagcgcatga gtgccgatac cgcgattgtc gtgctgacgg ctttcgggtc
     1801 gctcgaaggc gcaatcgagg cgattaaaca aggcgcctac gattatctgg cgaagccgtt
     1861 caagaaagag gaaatcaaac tcgtcgttca gcgaagcctt gatcactgta ggctggtccg
     1921 ggaaaattcg aagttccgcg aagagctaaa gcataaggac gactggtcac cgctggtcgg
     1981 aagcagtccg gcgatgctgg aagtgtacaa actggtggcg cgtgtgtcgg aaggcaagag
     2041 cacggtgttg ctgcaagggg agagcggtac gggcaaggag ctgattgccc gggctattca
     2101 tgccaacagt cctcgccgag acaggccgtt tgtgccggtg aattgcggag cgttgccgga
     2161 tacgcttttg gaatcagaga tgttcggcta tgagaagggc gcgttcacgg gagcggtcgg
     2221 gacaaaaacc ggtctctttg aatccgccaa cggcggcacg ttgtttttgg acgaaatcgg
     2281 tgaattgggg caggcactcc aagtaaagtt gttgcgcgtg atgcaggacc atgaagtccg
     2341 gcgcgtcggc ggaaccggtt cgatcaaggt cgacgtgcgg atcattgctg cgacaaatcg
     2401 tgatctcgag cagttcgtga aagaggggaa atttcgggat gatctcttct atcggctcaa
     2461 tgtcgtgcgg attaccttgc cgtctctgaa ggagcggcag gaagacattc cgatgctggc
     2521 ccatcatttc ctgcaaaagt gcggtgcggg ggccacgcgg gctgtgcggg gatttcatcc
     2581 cgatacgttg gcgttgctga aacagtatcg gtggccgggc aacgtgcgcg aattggagaa
     2641 tgccatcgag cgcgccgtgt cgctcagtca cgggccgctt ctgacgccgg aggatttgcc
     2701 ggcggccgtt catcaggcca gcggcgctgc cgaacagaag gtggagatgg cagagacggg
     2761 cgacgagatc ttgctgaccc tggaggaagt ggagaaacgc catctcatcc gagtgctcaa
     2821 ggagatgaag ggcaacaagg tcaaagcggc gaagattctc ggcatcgatc gccggacgct
     2881 ctatcgaatg gccgagcggt ttgggctcga ccttggagag gatgcagagg gaggagacaa
     2941 agacgcaggt tgagatcaag gctcggtgaa gagcacaagc cgaaaacctt taagctgagc
     3001 gcgagcgttc agagtatttg taaggcgttc ttgataagcc gcagttgatt ttccgcgctt
     3061 tggggcaggg ccgtcggcga gtcttcccag tttgtaaaga tcccgtcttt cccttcgtat
     3121 agtacgttca accagagtct ggtactttgc tcggcggccg atgtcacacg ggcctcgacg
     3181 cggctccggc ctgtgccgaa gcgccagcgc aagagccaat cccagggccc gctgatttct
     3241 tcgcgatagc cggttgaaag cgtttcatcg tccctccgat caacatcata accgccatct
     3301 gttagcacct gaatcaccgc ggcccgaaca gtttcgaccg gggcaggcag tgtgacatcc
     3361 acaatatccg gctcatgggc gagagggggg ccggcacagg ccgtcgttgt gacgaagagg
     3421 aaggcaagga ggcatcggca ggggagcgag ttcacttcgc tttcgtgtcc ttcaggcggt
     3481 agacaagatg ggtatccgtt tgggccaccc cttcaatcgc gtgaattcta ctgagcacca
     3541 attgcgtcaa ggcgtcctga tcgggcacct ctacaaccgt gatgatatcc ggcttgcccc
     3601 agcagggatc gatcgtcttt atttgcttaa tctcgcccaa ggcccgaacc acatgactcg
     3661 tttgcccggg cagcacatga attaacacat aggcacgatc ggccatgacc actccaccgt
     3721 ctaccggtgc atgaattgtg cagggtaccg ccggaatggc cggttcgttg ccaacgtagc
     3781 ggatacccgg cgtgcctgtc aatgtggcgg aggtaccgga tgcttttgct ttcttcatgg
     3841 gcggcctcat tttggtgcga cgagcacgtc gacggtaaac tggtcgctga tggtggcaag
     3901 atcgctctcc aaccctcgag ggttgccgat gcggccttcc gggtcatata cgaaacagaa
     3961 gagcgtggtg catcgttcct gcgcgcggta tcgttcaatg tcgacacgga gctgatccat
     4021 gaggtccttg gtgctcaggc cggtccgcgt tttcttgacg acaatcgcca gtctgtctcg
     4081 gtccaggagg aaggtcgtac gcggtgcacc gtttgtataa ctgggggccc attcgtcggt
     4141 gccgatatcg tcgaattgga cacgcaagag ggcatggagc aaatcctgca catccacttc
     4201 gtcctcgaca gctaccgtgg ctcgatattc cccgcggagc cgcaactgac gcgcaatggc
     4261 atgaaaccgc cagcagagtt ttctgcaaag ttcaaggtga tcgaccgctt gaagcgacga
     4321 atcgcttgtg acagcggtgg ctggacgcgg caccgaacga acttgagctg gttgagatgc
     4381 caagggaact tcctgcgtgg ccgagtgttg cggggaactg attgacggct ggattgtcga
     4441 gggatctgat gccggcagag ctggctcgga aactggcgtt gaaacgggag ctggtggtgg
     4501 agcgctgacc gatcgttgag tctgtggaat gggaggggca accgacgccg tgtcattggg
     4561 acgaaataac gtcgacactt gatcgggtgt gatctgtgat ggtttcggcg ttggcgccgc
     4621 tacctgctgc gacgctgtga attcggtcga tggaggcggc gtgatcgatg ctggtgcgga
     4681 cacagtcggt gaaggtggcg atgcggtcga tgtcgccccc atatcgacgt tcgtcgtcag
     4741 cgggacggac atggtaatgg gtggaggtgt cgccggtgct gctgatgtca tggtgatctg
     4801 ggcggtcgat gctgtgggag gaaccagcgt catctgcggt cgtccttgcg gagtagggct
     4861 tgatgttgct gacggttttt ctggtgcaga tggcttgagc ccttgcagct cgagtttctt
     4921 ttcctcaagc gtggcgatca gcattttcac gagactgacg gtttgtttgg tttcagccgg
     4981 ggtccccatc gccagtttat ggtgtcgatg tgcctgatat tcaggcgact tttcaccgaa
     5041 ggcgcgctta atgcattctc ggagttgcag ttcggttctg gctctcgcac cctcaatgta
     5101 agggaaccct tctcgtccga ggtcttcgac tttcggaaga taggattgga gctgctcgat
     5161 gctctgggtc aggtcttcga tggcggaagt tttagaccgc gaccgctcgg tttttgcacg
     5221 tgccatagag tgtgagaaaa gtgtagccca cgccctatgg cctgacaaga aaacacggga
     5281 aaccgagcag ccaacgacgc atctcgcctg ccgatcgaat ctctcgatag gccaatctac
     5341 gctaggctgc tatatcgagt ggcaattctt gctgcgcgct cacggctatc accggttggg
     5401 cctgctgtgc cgtggccgcc gaggccattg ccgccgcatg caaccggcgg ttctgttcca
     5461 cgacgggatc aaacacatgg ccgcagttga tgcagcgcca gccttccatc caccattccc
     5521 ctccactttc ctgaagatca agaaagtggt cgcgagccat gcagccctga caacgtcgac
     5581 aagtcatggt gccctccttt cggtgccaca tggttctagg gtagcggaga ggctctccct
     5641 tggttagacc ttaaaggagt acctcaaaag gagggtgata gaagagatta cttggttata
     5701 cagtttttgg agtcaaagca ggggctgcca gggtcgctag aaacgcgaga agttgcgttt
     5761 tgacggctgg ttccagagta tcgaatttca cgccgaacat gccccctttc gtccaacgta
     5821 catgagcacg agcgatgtga atggagatgg ctgagtcggc ggcggtgatc tggagcgcaa
     5881 cttgaacgcc ggtgtggacg ccttgggagc cctgaatttt gaggccctct tcagagagat
     5941 cgagcagatc gccggtaccg caaaattctt tggcaatgta tttcacagct acaaggcagg
     6001 ctggtttggt tcgaattttc cattcgctgc gcttcgacat cggcggctct ttcttggatt
     6061 cctaggcttt caacatgagt ataactgatg atttggtgag tgcaaggaga agaggcattc
     6121 cgagacgaca tcattcaagt aactggcgaa ggtgatgtgt gcgagggatt caggggagaa
     6181 tccgggagaa aatataccag gggcaactat agagaggcct cacgccgatg aggcgccggt
     6241 acgagcgagc ttggtttgat gccaccgaca cgaatcgacc gtgcgacccg cagtttagga
     6301 attactccta gtcctgcctc agacaacata ggcccacaag acacaacaag acaggctatt
     6361 gatgaaatgg cctgatggcc ctgatttgtc ctatcctggt agcagcgtgg taacagaaat
     6421 aaaatcttgt ggagtcactg ggagagggga attcgcactt gcgcaggatt tgccggaggt
     6481 tgattaactt tagttagcac ttcgttgcaa tagtgcttga cgagcaaaac ttcttcatgg
     6541 gtaaggggcc gatagatcgc gatgcctgca agaaggtgct cacatgcgct ggtaaaatta
     6601 ttcacgtcct ttgagagccg catgaaagac acctttcatt tcggcgaagc agcgctggaa
     6661 tgttatgagc gggacgcggc attttgagct ttctccgacg tcttgtaaag aggttcgagc
     6721 acaatttctt attatcacga gatagcaaca agagagagta agagggtaat cgaaaagcaa
     6781 atgagaaatt ttcagatcgg ccctgcggac ctccccccct tacgccgcga cccgcgattt
     6841 ggttgtcctc gctgagatga aattgcatgg ataatcaaga tgttgtaagc catgctggca
     6901 cgatactacc gtcgggacct gacctgatat agggaagaag accggccgcg gctatcagaa
     6961 gagtggcctt tatacagcga aaacagccct caagcagttc ggtgaccgtg ctatagacgg
     7021 tctcgaacag gaatggccaa ggcggcggtg aatcgcttgg atgcggaatt tttcgacgtg
     7081 attggagatg gtagcaaatc gccagaaatg gttgcagtcg gagaggtccc ctggaccgga
     7141 tcaattctgc aactgcccga agtggctgaa aaaattggtg cccccgacac gaattgaacg
     7201 tgcgacccgc ggtttaggaa accgctgctc tatccaactg agctacgggg gcgtttggga
     7261 tgctgaaaaa gacccctgct tggtttacgc gaccctcaaa ggctcaatga ccggaaacgc
     7321 aaatttcgct ttctcgcttg gccggggcca gcccgggaat gcgcatcttg gcgcgcagga
     7381 gctgggcggg tgggcaggaa gcctttttga acatcccaac agacgactcc tacgatactg
     7441 aataggcaat gaaaagtcta ggcgagtctt gagatcaacc gcagctgctt agggcttcgt
     7501 gttgtcgtcg ttcttcgatg aggcgagcag gtcctttttc gcaggaatct gagttaatag
     7561 atcagcgcga atttggaaga tgccctgcag gcgaagcccc atcgcatagg ccaggccgtt
     7621 cgcagcgttg ccaacagcca gcttgccggc gagttttccg tctcgtccgg ttgcgacaaa
     7681 ctctgcggcc gggcttacta acccataggg cgctaagggg ccggcttgtt tgacgacccg
     7741 ttcttctgcc ggaacattgg cgacacggct cacaaagaga tcggtgacct gctggttgag
     7801 cttctcggtt gggcgatcct ccaaaaccca ttccctgttt tggttgatga gaacaaaatg
     7861 ttccgcgcgt gtcgtcacag agagcatcgc gatgtcggtg taatcgacac cgaggaggcg
     7921 tttgtcctga aaagtgaaca gttccttcgt gagatctttg atggccgtgg ggttgatccg
     7981 gtacagaggg ccttccgggc cggtttccgc aatcgcttcg ccgcttgagg tgtccgattg
     8041 gtagagtttc accgttcggt cgccatccgc cgcgtgcagg gtaattcgta cttttggcgt
     8101 tgtcagggtc cgggcgagcg cgtcacgttc gggccctgga tccacaatcc ccagagcttt
     8161 caagtcttcc agtctgaaga gcagcattcg cacttcgttg agatccgcgt cggcctctat
     8221 cggatatcga attttccact tacccttggg cttttcatcg aggtgataca gcacgatttc
     8281 ggtggtagga taggtgaggc gaatgcgttc gatctcgctc cgcgccacac gcaggatatc
     8341 tttccggcga aaggtcatga gcgtcttgtt cacgaagtct ttcgccgcta aatcagtgag
     8401 caggagtgca tgatcggatt ctctgagtac gtacagggtg gacgacaatg ggccgctgtc
     8461 gccgatcgag agcgtttcct gtccgctggg tgcggtgatg gtgaccgtcg tgacaggttt
     8521 gtcgagtccg aagggggaaa gcgtcgtcgg gttctcttcc acaacccggc gaacggagcc
     8581 ggtgacaagc gcgcggacca ggttctgaac ttcgcgctgg tccgcttccg tccgcaaggg
     8641 agccgtcagc atccagccct tgtcctgggt gcgttcaaag acgaggtcgc cacgatcggt
     8701 cttgaagctc agccctgtga tggaggcctg atcgaggagg aggacttttt tctgcgccgt
     8761 tgcttggcgc tcctcagtct ccttggctgg gaactcgacg atgtagagat agagcccgag
     8821 ccccgcgagc acgagtgcca tgagcagcgt ggaccagtat cgcatgagtt acaaccggcg
     8881 gcgtttgcgc cagaccatga agccggcgat gacggtgaac gccgggagca gcaccacttg
     8941 aagatagagc agcgcccgct cttggagcgg attgggcgtg aacggcctga gcgcctcttc
     9001 cttcggaccg atggcgatca agtcacgctc ttcggcgagc cacccggccg tatggagaaa
     9061 gaagtcgctg ttgccgggaa aattcagaaa tgcattggtg gcgaacgtgg agttgccgat
     9121 tacgacgatg gccggccgtg gttttccttc cgcaagcgga gatttcggcg atagggctgc
     9181 cgccatcggc aacggtccct tcacgtcttc cttctcgtcc aggttcacta cccggcctct
     9241 gagatccgtt tcggcccagc tgttggggga ggtgcgggcc aacgggacga agtcccagtc
     9301 cttgcccacc tgctcgtcga aggtgagatg tcgggagagt gggaacagga cggcggctgt
     9361 caggtcttgg gtaatttcgt gttcggtaaa agtgcggacg aggagcgcgg tcaaatcgcc
     9421 ctgggccaag cggtcctgga gatcgacgag gactccgggg cctgctgtga gtccccaacg
     9481 tttcagtaga ggattgaggt ccgcttgcgt gttcggatcg atgaggagca ggagatgccc
     9541 accttgttgc acgtaggtat agatgcgatc ctgttcttca gtcgtgacgg accgttgcgg
     9601 gcccgccagg acgagaatcg ctgtgttgtc cggaacggcg gtgtccttga gtaggctcag
     9661 agtgccgacg tcatagccct gcttgatcag ggcttcccgg gcgacgaaaa accctgtccg
     9721 ctcccggtcg tccaggcttc gttcgccatg gccttcgaga aagagcacgc gtttcttgct
     9781 gctcttcgac acacggatca atcccccggt gagttcggct tccgacggcg tcgtgacccg
     9841 agcctggagt tcgccgcttt cgaatatcgc cgtatccgtt cttgatatgc cataggcctg
     9901 cgctattttg ggctgccgtt cgggatcaac gaattcaacg gtgactttcg gactggcttg
     9961 ccgatagcta tccaatcgtt ctttataaga ctgatagccg ggatccttct cgcgggtaaa
    10021 gacggtaatc ttcacgtcgc gcggcaggct gcggatgatc cgatgagtct gcggcgagag
    10081 agagaagttc tggttttccg agaagtccca acgaactgag tgtcgtgaag ctaggaaatt
    10141 aacgatcacc agaatgcaga caaacaacag gaccatcaag aggctgttgg cacccatgcg
    10201 cgtggcgcgc cgagttgaaa atgctttcac ggtctggaaa tgcaggacaa aaaacaggat
    10261 caggcacagc agcgcgagtc cttctgccag ggtgacgagc cacaacttgt ccggcgcgag
    10321 gctgtagccg accatcccgg ccacagctag ggcgattccg atgacgccga gtggaaggga
    10381 gtgaagattc atcgccaccg tgccgaatcg acgacccggt gcgtgaggaa cagcatcaac
    10441 acgagggcgc tgaggaagta caccaggtct ttcgtgtcga ttaatcctcg cacgaggtga
    10501 tcgtaatgtt ccatgaagga gaggtaggag atggcttgtc ctgccgttgt atcgccaagg
    10561 agcgagccga ctccggctag aagccacaga atgaggagca ggccgaaact ggtgaaggcg
    10621 gcaacgatct gattttctgt caacgcggag gccaggaccc ccaccgacag gaacagagcg
    10681 cccagcagcg cgagtcccag atagccggtc aagactgggt accagtcgaa atcgctgaac
    10741 agcgacagca cgagcgggat caatcccgtg agtccgagga gccccagaaa gacaagcaag
    10801 acgctcataa atttccctgc cacgatttca ttcaggccga cgggggaagt catgagaaat
    10861 tcgaatgtgc ggagcttccg ctcttcggca aacagccgca tcgtcagcat cgggagtatg
    10921 at
//
LOCUS       DATIFR010000044         5424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1748226, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000044 DATIFR010000000
VERSION     DATIFR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748226"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VLD60_02265"
     CDS             complement(<1..499)
                     /locus_tag="VLD60_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB43802.1"
                     /translation="MLRPSRVMVVDDDIETLALLREVVTKEGYEVVTAEDAESAMRHL
                     AEWQPDLVITDIHMPGMDGLALLAAVREKAPDILVILLTAYGSLKTAVDAIKAGAFDY
                     LSKPFVVEDIRLVVRRAIEHKKLVGENRSLREQLRERYRFDNLVGSSPGMVEVYKLVA
                     RVAGSD"
     gene            complement(499..2112)
                     /locus_tag="VLD60_02270"
     CDS             complement(499..2112)
                     /locus_tag="VLD60_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB43803.1"
                     /translation="MLSWWHQLSLQVKITAIIVLIFMVSAVMTEWIEARLIQRTVEDN
                     VREAALAVGRSVDQNVISLAQLSNRDARTKELEKILANLPDLLNIVLYASPTESGESF
                     VPVASAGPTELPRQEGAPDLVHGVREERRPLIDYADRPKTHRVFLAAPIFLKGVVVGV
                     TYAEFSTLQMDDALDALQAASRNRRLLTWLAIVVAINLFLYFKFHRPVQRVLSAIKAV
                     SGGTMTAAVPVDEQDELGELAGTFNDMVKTIRETTEHNAKLKESLRAMNDNLQSKVIE
                     ATTEVMQKNRALAHTNELLSIAQRDAARAQRLSAIGQMAATVAHKIGTPLTALSGHIQ
                     LMAEDPTLSTESRQRLQTVEAQIERTSKIIQDMLLYARRPEPVRAPLDLNACVAECIA
                     LLQPELERRHIALVTEWSQQVEKVDADTQQLQEVFINLIENALDAMPAGGTLVVRTSP
                     HDESKHPDHRSFVEVEFADTGCGMDAEQIAQIFQPFFTTKKSGRGTGLGLAIALETIR
                     AHGGQINVESEPGHGARFTIVFPTDGGRS"
     gene            2385..2798
                     /locus_tag="VLD60_02275"
     CDS             2385..2798
                     /locus_tag="VLD60_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43804.1"
                     /translation="MAGRILLVESDAVLGAVVGEVLHHSGHEVISVRTLQGQGEHIPS
                     GSTVILDLDTVSAETELACLSSLQPPSNPMPMPIVLIGLEAPEDLGHLLREHLGRRQA
                     KHLTWVQKPFRNEELIAAVQRVNGRGPAGLPFSGE"
     gene            3173..3454
                     /locus_tag="VLD60_02280"
     CDS             3173..3454
                     /locus_tag="VLD60_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43805.1"
                     /translation="MSRLISGLMVLLCCLIFAMPGALAQIETSLQTIKGDLLSVKGDV
                     YLIKDVFGRLVYLHVDKNTKRERMLVPGERIEADVVMTGERVVALRPAK"
     gene            3491..4582
                     /locus_tag="VLD60_02285"
     CDS             3491..4582
                     /locus_tag="VLD60_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB43806.1"
                     /translation="MRIAQVAPLWESVPPKLYGGTERIVSYLTEELVRLGHDVTLFAS
                     GDSLTAARLEPICAHALRLNTGIFNRDAPMTMLMEQALGKPGVFDIIHSHLDFLGFPL
                     GRRNATPMVTTFHGRLDLPELQPVFREYAEMPMVSISDAQRRPVSWANWQGTVYHGLP
                     QDLYTFHEEPGRYLAFLGRIAPEKRPDHAIKIAKRVGLPLRIAAKVDPMDREYFQSEI
                     QPLLTHPLVEYLGEITDAEKNEFLGGAAALVCPYDWPEPFGLVLIEALACGTPVLAYR
                     RGSIPEVIEDQSTGFVCEGLSEMVAAVDRIPEIDRHRCRRAFEQRFSVERMALDYLRV
                     YEMLVAKTQEEPEAEATSVVSWPALAASA"
     gene            4674..5024
                     /locus_tag="VLD60_02290"
     CDS             4674..5024
                     /locus_tag="VLD60_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43807.1"
                     /translation="MKLVQPLNTTGIAVCLTVSIMLITDTYVEAGSVSDSKTVTGEVS
                     KVEGEFHMAKDIRGEDTLKIVDKSYVITDQDGKELRLELRNDTKVRNRVNPGDKIEAK
                     ISSEGHTLSVTRLK"
     gene            5101..>5424
                     /locus_tag="VLD60_02295"
     CDS             5101..>5424
                     /locus_tag="VLD60_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB43808.1"
                     /translation="MVEQGLTVEERTTSGWQLLSTKNFGLLFSGQVVSQIGDSMNKVA
                     LLWFVYDLTGSAFKMTLIGLLQTIPPLVFGPLIGVYLDRVRRKPVMIGVDLIRTVLVL
                     LIPLLY"
BASE COUNT         1132 a         1485 c         1588 g         1219 t
ORIGIN      
        1 tgtcagatcc cgcaacgcga gcaacgagtt tatacacctc gaccatcccg ggactgctgc
       61 ccacaaggtt gtcgaagcgg tatcgctcac gcaattgctc gcgcaacgaa cgattctctc
      121 ccaccagttt tttgtgttcg atcgctcgcc gaaccaccaa tcgaatgtcc tctacgacaa
      181 acggtttgct gagatagtcg aaggccccgg ccttgatcgc atccacggct gtcttgagcg
      241 atccataggc ggtcagcaag atcacgagaa tatccggcgc cttctcgcgc acggcggcca
      301 gcagggccag cccatccatg cccggcatat gaatgtccgt gatgaccagg tcaggctgcc
      361 attcggcaag atgccgcatc gccgattccg catcctcagc ggtcacgacc tcgtagccct
      421 ccttggtcac gacctcgcgt agcaaggcca atgtctcgat gtcgtcgtcc accaccatga
      481 cgcgagatgg ccgtagcatt agctccttcc tccatcggtt ggaaacacga tcgtgaaccg
      541 ggctccatgc cccggctcgc tctcgacatt gatctgtccc ccgtgcgccc gtatcgtttc
      601 cagcgcgatc gccagaccca gtccggtacc gcgcccagat ttcttcgtcg tgaaaaacgg
      661 ctgaaagatc tgcgcgattt gctcggcgtc catcccgcag cctgtatcgg caaattccac
      721 ttcaacgaac gatcgatggt ctgggtgctt cgactcgtca tggggagagg tccgtacgac
      781 gagagttcca ccagccggca tcgcatcgag cgcgttttcg atgagattga taaatacttc
      841 ctggagctgc tgggtatcgg cgtcgacttt ttcgacttgc tggctccact ccgtgacgag
      901 cgcgatgtgt ctgcgctcga gctccggctg gaggagcgcg atgcattccg caacacacgc
      961 attcaaatca agcggagcgc gaaccggctc cggccgcctg gcatacaaca acatgtcttg
     1021 gataattttc gatgttcgtt cgatctgcgc ctccaccgtc tgtaaccgtt gtcgcgactc
     1081 tgtactcagg gtcggatctt cagccatgag ctggatatga ccggacagtg cagtcaacgg
     1141 cgtgccgatc ttgtgtgcga cggttgcggc catctgcccg atcgccgaca gacgctgcgc
     1201 gcgtgccgca tcgcgctgtg cgatcgagag gagctcgttc gtgtgggcga gcgcccgatt
     1261 cttttgcatc acctcggtgg tcgcctcaat gactttcgac tggaggttat cgttcatcgc
     1321 tcgcaaggat tccttcagtt tggcgttgtg ttcggtcgtt tcccgaatgg tcttcaccat
     1381 gtcattgaat gtgccggcaa gttcccccag ttcatcctgt tcgtcaaccg gcacggccgc
     1441 ggtcatggtt ccccccgaga ccgccttgat cgcggacagc actcgctgca cgggacgatg
     1501 gaacttaaag tagagaaaga ggttgatggc aaccacgatt gccaaccacg tcagcagtcg
     1561 cctgtttcgc gaggcggctt gcaaggcgtc cagggcatcg tccatttgca gggtcgagaa
     1621 ctcggcatag gtcactccga ccacaacgcc tttcaaaaag atcggggcgg caaggaaaac
     1681 gcggtgagtt tttggccgat ccgcataatc gatcagtggc cgccgttcct cgcgcacccc
     1741 atgaacgagg tcaggagctc cctcttgacg aggcaactcg gtggggccgg cgctggcgac
     1801 cggtacaaaa ctttctcccg attcagtcgg ggatgcgtag agcacgatgt taagcagatc
     1861 cggcagattc gcgaggatct tctccagttc tttagtgcgc gcatcccgat tcgatagctg
     1921 ggctagactg atcacgttct gatcaacgga tcgcccgacg gccagtgccg cctcccggac
     1981 attgtcttca accgttcttt gaatcaagcg ggcctcaatc cattcggtca ttactgcgga
     2041 aaccatgaag atgagcacaa tgatggccgt gatcttgacc tgaagactga gttggtgcca
     2101 ccagctcaac atgattccat ggtagtcccc ctgggttcct tgttcaatcc gattccgaga
     2161 caagatgtct ggcccggaat aacttgtctc ttattttgat gagcaggcct ccgctagtca
     2221 tgtctccctc gtaccggtca ccatggtaaa accctgaatc ttccgcagcc ttacagccgt
     2281 gaaagccaag gaaaggcaag gagacgcaag ggatctttct caggcatcta gcttgctcaa
     2341 tcccgctacg catggtgcta gtcgaacttc aggagtggct caacatggct ggccgaattc
     2401 ttctcgtcga atccgatgcc gtgttgggcg ccgtcgtcgg cgaggtgctc caccacagtg
     2461 ggcatgaggt gatctccgtg agaacgttac aaggccaagg tgagcacatt ccttctggga
     2521 gcaccgtgat tctcgatctc gatacggttt ctgcagagac agaattggcc tgtcttagtt
     2581 cgctccagcc accgtccaat cctatgccta tgccgattgt cttgataggt ctggaggccc
     2641 ccgaagatct agggcatctt ctgcgcgagc atctcggtcg gcggcaggcc aagcatctga
     2701 cgtgggtgca gaagccgttt cggaacgaag aattgattgc tgcagtgcaa cgggtgaacg
     2761 ggaggggccc ggcaggatta ccgttttccg gggaatagag tttcggggaa atgtcaccag
     2821 ggcggcatgg cgaaactcca gctttcccga tggtgacgcg atgcaggtgc cgagtacgac
     2881 acgtagggaa ggaatctcag gcaatgcgca atccggcacg tatcattcac gagaagacga
     2941 aagatcctgg caggatcaag accgaatggt tgggtgtgga ggcatgtgtg cctcttcttc
     3001 cacggctggc ggcgggagct cggttcatgc tctggagccg gcggttgcct gcccgcgtcg
     3061 ttctatcctc gttctcgtcc ggattcgcaa tgggcggctg cctggccttg ttcgcttgtg
     3121 gatggtttgg gagccgtctc tgtcgggttg gagatggcaa gtgaggtgaa ccatgtcgag
     3181 attgatcagt ggtttgatgg ttctgctgtg ttgtctgatc ttcgccatgc ccggtgcact
     3241 cgctcagatt gagacgtctc tgcaaacgat caagggcgat ctgttgtccg tcaaaggcga
     3301 cgtgtatctg attaaagacg ttttcgggag gctcgtgtat ctccacgtcg acaagaacac
     3361 gaagcgagag cgcatgttgg ttccagggga aagaatcgag gcggatgttg taatgacggg
     3421 tgagagagtc gttgcacttc gaccggcgaa ataggccaat gttcatcata ataacgaaag
     3481 gaacagtccg atgagaattg cccaggttgc gcctttgtgg gagagcgttc cgccgaagct
     3541 gtatggtgga acggaacgaa tcgtgtcgta cctgaccgaa gaactcgttc ggctcggtca
     3601 cgacgtgacc cttttcgcga gcggagactc gctcaccgcg gcacgcctcg agccgatctg
     3661 cgctcatgcg ttgcggttga atacagggat cttcaatcgc gatgcgccga tgacaatgct
     3721 gatggaacag gcgttgggga agcccggcgt gttcgacatc attcactccc atctggattt
     3781 cctgggcttt cccctcggga ggcgcaatgc gacaccgatg gtgactacgt tccatgggcg
     3841 gcttgatttg ccggaactgc agccggtgtt ccgtgagtat gcggagatgc cgatggtgtc
     3901 gatttcggat gcgcaacggc ggcccgtctc ctgggccaat tggcaaggca ccgtctatca
     3961 cggcttgccc caggaccttt ataccttcca cgaggagccc ggaaggtatc tggccttttt
     4021 gggtcgcatc gcccctgaga aacgaccgga ccacgcgatc aaaatcgcga aacgggtcgg
     4081 cttgccgctg cggatcgccg cgaaggttga tccgatggat cgagaatact ttcagtctga
     4141 aatccagccg ttgctgaccc atccgctggt cgagtatctc ggggagatca cggatgccga
     4201 aaagaatgag ttcttgggtg gcgcagcagc gttggtctgt ccctacgact ggccggagcc
     4261 gttcggtctc gtattaatcg aagctctcgc ctgtggcacg cctgtgctcg cctatcggcg
     4321 tgggtccatt cctgaagtca tcgaagacca atccactgga tttgtctgcg agggcctcag
     4381 cgagatggtg gcggcggtgg atcgcatccc ggagatcgac cggcaccgat gtcgtcgggc
     4441 ctttgagcag aggttcagcg tcgagcgaat ggcgttagac tatctccgtg tgtatgaaat
     4501 gctggtcgcg aagacccaag aggagcctga ggcagaggcc acgagtgttg tctcctggcc
     4561 ggccttagca gccagtgcgt aactctcgat cacataggga gaattggaaa gtggaagagc
     4621 tggaggaaga aggggaataa acgcattctc cagctgtgcg gtaggaggtg agtatgaaac
     4681 tcgtacagcc tctcaatact accggtatag cggtgtgcct gactgtgtcc atcatgctga
     4741 tcacagacac atacgtggag gcgggatcag tctccgactc aaaaacagta acaggtgagg
     4801 tgtccaaggt tgagggtgag tttcatatgg ccaaggatat ccgaggtgag gacacactca
     4861 aaatcgtgga caaatcctat gtcattaccg atcaagacgg gaaagaactg cgccttgaac
     4921 tgaggaatga tacgaaagtc cgaaataggg ttaacccagg agacaagatc gaagccaaga
     4981 tctcatccga gggccacacg ctttcggtca cgcgactcaa ataacagggt aaggggtagc
     5041 cacgaaccgt aaaccacgat gcgcgaggcc gagcgcgctg tttgttgagg agatttcatc
     5101 atggttgagc aaggtctgac agtggaggag cggacaacgt ccggttggca gttgctatcc
     5161 acgaaaaact tcggcctgct cttttccggc caggtggtat ctcagatcgg cgacagcatg
     5221 aataaagtgg ccctcctgtg gtttgtgtac gacctgacgg gttcggcatt caagatgacg
     5281 ctgattggat tgctgcaaac gattcctccg ctggtgttcg gtccgctcat cggtgtgtat
     5341 ctcgatcggg taagacgcaa accggtgatg atcggagtgg atctcatccg gaccgtactg
     5401 gtcctgctca tcccgctctt gtat
//
LOCUS       DATIFR010000045         3698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_982920, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000045 DATIFR010000000
VERSION     DATIFR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982920"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="VLD60_02300"
     CDS             <1..1832
                     /locus_tag="VLD60_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HSB43809.1"
                     /translation="ALVNMPFSYSKYPSIQLGTLSALLKSKGIAVDCHHLNVRFAHKI
                     GVPLYEMICEKRALFGEWLFSHLLFRDNPKRAEYPRVFKPVFEQLAQQSGQPIGYFEE
                     LSTRIAPQFLMWALTAFDWEQYKLVGFTSTFDQNVASLTMAKLIKDLYPDVKIVFGGA
                     NYDGEMGMEYFRAFPFIDHVVVGEGEETFPALVRTIVDGNDGAFPDGVAYRQGEKICL
                     SPNRSLFSDFTKTGPPDYDDYYHLLAELGDAAQGLDRILLYEGSRGCWWGEKHHCTFC
                     GLNAQSMKFRAKSFDQVAREMTHLSHRYDTVRFRLVDNIIDMKYVEHLFGKFAADHCD
                     LDVFIETKSNLHKSQIRTLAVGGVKCMQPGLESLSLNQLKAMDKGVTPMQNIICLKWS
                     FYYRVTVSWNILLGFPGESNEDYQRQIDLIPSLVHLQPPEATGKFWLERFSPYYTRPH
                     EYGVRITGPGMAYEYVYDARQVDLKKIAYDFEYELDHWPVDPHVYQELIAAIEGWQRL
                     HASSDRPFMYYSKALDYVTVYDGRNPKAPTRQRYDGLAARVIEICNEAARSPDQIRAE
                     IAGLVDASADTLTPVLNDLTAKRVLYEERGKYFTLAIPENPYL"
     gene            complement(1851..2183)
                     /locus_tag="VLD60_02305"
     CDS             complement(1851..2183)
                     /locus_tag="VLD60_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HSB43810.1"
                     /translation="MDTYYHPHDLGKFADMGKGSPALWEKFMGYYSAVFADGALTERE
                     KVLIALAVAHTVQCPYCIDAYTQASLEKGSNIEEMTEAVHVACAIRGGASLVHGVQMR
                     NVAEKLSM"
     gene            complement(2271..2555)
                     /locus_tag="VLD60_02310"
     CDS             complement(2271..2555)
                     /locus_tag="VLD60_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43811.1"
                     /translation="MASSTPPSGQSNGKPSQPAGGESATWDVELLKVLVSRKGTQVDA
                     QWAIHPQLKQDLLPHEWQEVTDLMGKVAGIVGTRFSQVLSQADPEPPSNA"
     gene            complement(2658..3485)
                     /locus_tag="VLD60_02315"
     CDS             complement(2658..3485)
                     /locus_tag="VLD60_02315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSB43812.1"
                     /translation="MSKSLDTTCSPEALLSVAVEAARAAGTILLNYAKSGFRITHKSP
                     INLVTDADHAAEQCVIDHIRAQFPAHHILAEERGSDVQSGSHYHWVIDPLDGTTNFAH
                     GFPLFTVSIGVELDGQGLIGVVYDPTRDELFTAHAGSGARLNGQPISVSITDQLDRAL
                     LATGFAYDIRDTPNNNLDHFARFALKAQGLRRTGSAALDLSYVAAGRFDGFWEVKLSP
                     WDMAAGVVILKEAGGKVTDFAGHAHSVYQPELVASNGRIHQAMLDVLCEGRTRQITT"
BASE COUNT          796 a         1002 c         1067 g          833 t
ORIGIN      
        1 tcgcgctcgt caacatgccg ttcagctatt cgaagtaccc ctcgatccag ctcgggacgc
       61 tctcggccct cctaaaatcc aaaggcatcg ccgtcgactg ccaccatctc aacgtccgat
      121 ttgcgcacaa gatcggcgtg ccgctctatg agatgatttg cgagaagcgc gcgctcttcg
      181 gcgaatggct cttttcgcac ctgctttttc gggacaatcc aaaacgggcg gaatatcccc
      241 gcgtgttcaa gccggtgttc gagcagcttg cgcaacagag cggccagccg atcggctatt
      301 tcgaagagct atccacgcgg attgccccgc agtttctcat gtgggcgctg acggccttcg
      361 actgggaaca gtataagctg gtcggcttta cgtcgacttt cgatcagaac gtggcgagcc
      421 tgacgatggc gaagctaatc aaggatctgt atccggatgt gaaaatcgtc ttcggcgggg
      481 cgaattacga cggcgagatg ggcatggaat actttcgggc ctttcccttt atcgatcatg
      541 tcgtggtcgg tgagggagaa gagacgttcc ccgctcttgt ccgcacgatt gtggacggga
      601 acgatggggc gttccccgac ggagtggcgt accggcaggg agagaagatt tgtctctcgc
      661 cgaaccggtc tctgttctcg gatttcacca agaccggtcc gccggactat gacgattatt
      721 atcatctcct tgctgaactt ggagatgccg cgcagggact cgatcgcatc ttgctctacg
      781 aaggttcacg cggctgttgg tggggcgaga aacatcattg taccttttgc ggattgaatg
      841 ctcagagcat gaagtttcgc gcgaaatcct tcgatcaggt tgcgcgagag atgacacatc
      901 tttcacaccg gtacgatacc gtgcggtttc gactcgtcga taacatcatc gacatgaaat
      961 acgtcgagca tctcttcgga aagtttgccg cggatcactg cgatctggac gtctttatcg
     1021 aaacgaagag caacttgcac aagagccaga tccgcacgtt ggcggtgggc ggcgtaaagt
     1081 gcatgcagcc cggattggag agcttgagtc tcaaccagct gaaggccatg gacaagggcg
     1141 tcacgccgat gcagaacatc atttgcctca agtggagttt ctactatcgt gtgacggtgt
     1201 cgtggaatat tttgcttggt tttccagggg agagcaacga ggactaccaa cgccagattg
     1261 acttgatccc ctcgctggtg catttgcaac cgccggaggc cacggggaag ttttggctgg
     1321 agcggttcag tccctactat acgcgaccac acgagtacgg ggtcaggatc accgggccgg
     1381 ggatggccta tgagtatgtc tatgatgcgc ggcaggtgga tttaaagaag atcgcgtacg
     1441 atttcgaata tgaactcgac cattggccgg tggacccgca tgtctatcaa gaattaatcg
     1501 cggcgattga aggctggcag cggctgcatg cctcttcgga tcgcccgttc atgtactact
     1561 ccaaggcgct ggattatgtg acggtctacg atggccgcaa cccgaaagcg cctacgcgcc
     1621 agcggtatga cggattggcc gcgcgtgtga ttgagatctg taatgaggcg gctagaagcc
     1681 cggatcagat tcgagcagaa attgccggtc tggttgacgc gagcgcagac acgctcaccc
     1741 cggtcttgaa cgacctcacg gccaagcgag tcctttacga agagcgcggc aagtatttca
     1801 cgctggcgat ccctgaaaat ccctatctgt agatctgcgt ctcaaaccac ctacatcgac
     1861 aacttttcgg caacgttccg catctgcacg ccatggacga gcgaagctcc gccgcggatg
     1921 gcgcaggcca catgcaccgc ttcagtcatc tcttcgatgt tagacccctt ttcgagcgag
     1981 gcctgcgtat aggcatcaat gcagtaggga cattgcacgg tgtgagcaac ggccagcgcg
     2041 atcagcacct tctcgcgctc ggtcagagcg ccatcggcaa aaaccgcgct gtaatacccc
     2101 atgaacttct cccacaaggc cggactgccc ttgcccatgt ctgcgaactt accaaggtcg
     2161 tgcggatggt agtaggtgtc catagtgact cctcgttcga aggttaaggc tggaattgag
     2221 gctgaagagc tggaatgaaa ctgcgctgag gctctctcag cttaagcccg ctaggcgttg
     2281 ctgggtggtt cgggatccgc ttgcgagagg acttgcgaaa accgggttcc gacaatgccc
     2341 gcgactttcc ccatcaagtc agtgacctct tgccattcgt gcggcagcaa atcctgtttc
     2401 agctggggat ggatggccca ttgggcgtcg acttgcgtgc cctttctgct gacgagcact
     2461 ttgagcaatt caacgtccca ggtggcggac tcccctccag ccggttggga tggttttccg
     2521 ttagattgtc ctgaaggagg tgttgagctg gccatgcctt gatactcctt gtggctgata
     2581 atgtgtaatc ataccctatc gcgattctct ctgtatatcc ttgtgcaaac ctttgtgggc
     2641 ggctgtccga atgagtttca ggtagttatc tggcgagtac gtccttcgca gagcacgtcg
     2701 agcatcgctt gatggatccg accgttgctc gcgaccagtt ccggttgata cacagaatgg
     2761 gcgtgtcccg cgaagtcggt cacttttccg ccggcctcct tgaggatgac gacgccggcc
     2821 gccatatccc aggggctgag tttcacctcc caaaatccgt cgaatcgacc cgcggccacg
     2881 taactgaggt cgagcgccgc gctcccggtt cgacgcaggc cttgagcttt cagcgcgaat
     2941 ctcgcgaagt gatccagatt gttgttcggg gtgtcacgga tgtcgtacgc gaaacccgtc
     3001 gccaggagcg cacgatcgag ctgatccgtg atcgacaccg aaatcggctg tccgttgaga
     3061 cgcgcgccgc tacctgcgtg cgccgtaaag agctcgtcac gggtcgggtc atacaccaca
     3121 ccgatcaggc cctggccgtc caattccacg ccgatagaca ctgtaaagag agggaatccg
     3181 tgggcaaagt tggttgtgcc gtcgagagga tcgataaccc agtgatagtg agaaccggac
     3241 tgcacatcag agcctcgctc ttctgccagg atgtgatgcg cgggaaactg ggcgcgaatg
     3301 tgatcaatga cacattgttc ggcggcatga tcggcgtcgg taacgaggtt gataggactt
     3361 ttgtgcgtga tgcgaaatcc ggacttcgca tagttcagca aaatagtgcc cgccgctcgg
     3421 gcggcttcga cggccacgga caatagagct tcaggcgagc atgttgtgtc gagagacttc
     3481 gacataggcg ggcatcatag catgggatcg gagtgagacc aggctgcttg catcagtttc
     3541 tatcagtagc aggatagatc gaaatcaccg ctacaaatag gtggccatga tcatccgtca
     3601 cgggttcgat aatcacgcaa ggcattacaa acacattctc tattgacttg cttcacaagc
     3661 aaggatataa agcaagcatg cttgttaagg agcccgcc
//
LOCUS       DATIFR010000046        13171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_218480, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000046 DATIFR010000000
VERSION     DATIFR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218480"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="VLD60_02320"
     CDS             complement(<1..2232)
                     /locus_tag="VLD60_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HSB43813.1"
                     /translation="MMELPGLPPKQGLYDPQHEKDSCGIGFVVNIKGQKSHRIVRQGL
                     QVLENLTHRGAQGCDPCTGDGAGILLQVPHEFLKRAAGDAGVILPDAGEYGVGQLFLP
                     PNQSARQQCEKLFGKIIEDEGARLLGWRDVPVKSDAIGVQARKTEPFMRQVFIARDVL
                     NEAQFERKLYVIRKRVEKAIKESAIQDREYFYIPSLSANTIVYKGLLLPYQMAAYYQD
                     LTDSSMVSALALVHSRFSTNTFPTWPLAHPYRYVCHNGEINTLKGNVNWMKARQGRLH
                     SDLFGQDMEKLFPIVSENQSDSACLDNALEFLLLGGRSLPHAMMMLIPEPWVANSQMD
                     LDRRGFYQYHAAMMEPWDGPAAVCFTDGKMIGATLDRNGLRPCRYQVTTDGMVVLASE
                     AGVLPAEAKDIRMKGRLMPGRMFLVDTVQGRIIDDEMIKADIVGRKPYRSWVTQYGVS
                     LDELPEPLNVPQPDHPTIRQRQQAFGYTVEELKMVITPMIVTGEEAVSSMGTDTPLAV
                     LSDRPQLLFKYFKQLFAQVTNPPIDPIREQLVMSLVTNIGPKPNLMEESPESCRRIKV
                     QQPILTNADLQKIRGISDPHFKSKTLRMLFRVAEGPDGLGAAVDDMCRQASQAIKDGY
                     KFLILSDRGVNEEWAPIPSLLGISAIHHHLVHECTRTEVGLILETGEPRDVHQFACLI
                     GYGAGTINPYLVFESLVDMERDNYLPEGLDAQTAEGKFIKAINKGLLKIFSKMGISTV
                     QSYC"
     gene            2495..3454
                     /locus_tag="VLD60_02325"
     CDS             2495..3454
                     /locus_tag="VLD60_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSB43814.1"
                     /translation="MWDAYRHELDGVEDQVRRNLNSSVTLVNTVAAHILNSGGKRIRP
                     LLLLLSARLCGYGGRDHHQLGSLVEFIHTATLLHDDVVDDADIRRGRRTARKVWGNQI
                     SILVGDYLYSKAMCQVVEFRSQGINEVLAEACKKMAEGEVLQLYYNGNPAMPETDYLT
                     IVEHKTAGLIAASCRMGAILAEASEVQQDALFRFGQYLGIAFQVADDTLDYTANGESL
                     GKTLGQDLRQGKATLPLLHLLNHCSEADRQMIKDRMETRTLTEEDLSRLIRLMEEYGS
                     INYALGRAQAYIAAAKRDLDSFEDNTAKRALSVAADYMVTRDR"
     gene            3578..4900
                     /locus_tag="VLD60_02330"
     CDS             3578..4900
                     /locus_tag="VLD60_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HSB43815.1"
                     /translation="MAQIIPFRGTLYNPTVVGAVRDVVAPPYDIIDAAGQKALHDRHP
                     QNIIRLELGLDQPGDNPRNNRYTRAADMLHDWLKSGALKRDAQPAIYYHTIEYAPPAS
                     KPGAPKKVLRGFLVTTKLEALDSGHIYPHENTRAAAKTDRLNLLQACKANLSPIWLLY
                     SDPQNAILSLLENATKGTPARIDFQDDSSCHQRLWVVTDPAVLKQIVEIMQNKPLFIA
                     DGHHRYETALNYQRLRRQQAGSPDGLQPYDGVLMLLAPLEDPGLTVLPTHRVTTTPLP
                     PYQKVKALMGEKFEIQEFPFTANTQAAVRAQFIEALRTNGQTTPTFGLAIKGESRYVT
                     LALKAAHRPTAQDSPRAKLDVSLLQQLVVAVLCPTQEEQEAILYTKDDHEALDWVANG
                     TGTGALLLNATKVSEVQAVATAGERMPHKSTYFYPKPLTGLVINVMEE"
     gene            5007..6371
                     /locus_tag="VLD60_02335"
     CDS             5007..6371
                     /locus_tag="VLD60_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB43816.1"
                     /translation="MKAKILIVDDDPDIATMLEDRLQASDYGTVTARDGVQALEMVEQ
                     EAPHLMLLDLDMPRLTGLEVLKRLPKVRPAEDLPVIVMTAHGSIEAAVEAMKDGAYDF
                     LTKPLDKDHLLIVIDKALERDSLKRQVACLKSEVDGRYASIVGTSAKIRTVLDAAQRA
                     AKSDASVLLLGESGTGKELLARSIHQWSPRHAMPLVVINCVALTETLLENELFGHERG
                     AFTGADRLQKGKLEMADGGTVFLDEIGDMSLPLQSKLLRVLQDREFHRVGGTRLVSVN
                     IRVIAATNKELKQAVKAGDFREDLFFRLNVISLTLPPLRERLEDFPALAKFFLNRHAR
                     EAKRPGMMFSPASMDALAHYTWPGNIRELDNVIARAVILSHTDTIEPDVLALDFNSAT
                     GKSDALPYVMLPYHESMDAHSRHIIEQALKQAEGNQTKAAERLRLQRTYLARLIKQKQ
                     SKFI"
     gene            complement(6495..7238)
                     /locus_tag="VLD60_02340"
     CDS             complement(6495..7238)
                     /locus_tag="VLD60_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HSB43817.1"
                     /translation="MKAMILAAGLGTRLRPLTNTIPKPLLPIAGTPLIVWNLLLLKRH
                     GFHDVVINLHHLGPMIEQALGNGSKYGLRIYYSHEPVILGTGGGIKQAEPNFSGEPVL
                     VLNGDTLFELDLEALSAFHHQRKAAATLVLRSDAEAARWGLVEVGPDERIVRITGRGK
                     SEAGPTQPRMFAGIHILHPRLLRDVPKGKESSIIDAYVSAIQRDEVVLGYDLEGYWSD
                     IGTAQRYAEAEHDANAGLISLQARQPVQL"
     gene            complement(7323..8426)
                     /locus_tag="VLD60_02345"
     CDS             complement(7323..8426)
                     /locus_tag="VLD60_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSB43818.1"
                     /translation="MTTIDPAAPKKPLALPDRALVAETVRTKLPFRGTVKKLTALAGD
                     ASNRRYFRIELSEQTARPVILMQLAEPEAFKQSEEAVSGAAHQINDLPFLNIQAHLAK
                     AGVPVPSLYFYDQVAGLLYLEDFGDVTLSDACRDATMTELEARYKLAVDILIQLQSKA
                     TSPADPGCLAFNRSFDVPLLMWEFDHFLEYGIVARQEKPMSGDDQAVMRDEFQKISEL
                     LAGQPRVFVHRDYHSRNLMVDGHRLGVIDFQDALMGPATYDLASLLRDAYIALDEALI
                     DRLIDYYLDRLESGRQVWTNREAFRRLFDLTSIQRNLKAAGRFVYIDRVKGNPKFLAD
                     IPRTLGYVKRNLHKYPELFTLRKLMGAYVPELQ"
     gene            complement(8461..8892)
                     /locus_tag="VLD60_02350"
     CDS             complement(8461..8892)
                     /locus_tag="VLD60_02350"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HSB43819.1"
                     /translation="MEKVLSATGVSSRYPILWYWLPVLLYAMLIFYLSSLPHPEDELP
                     EFVFKKLSDKFLHLVEYAVLGLLCYRALRWATGPSVARRAVIITIMAVSFYGMTDEVH
                     QAFVPFRESSWLDWLADTMGGVIGAVGLRRFTERDTRADLP"
     gene            8970..11168
                     /locus_tag="VLD60_02355"
     CDS             8970..11168
                     /locus_tag="VLD60_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HSB43820.1"
                     /translation="MKTARVCFLWHMHQPYYTDPVTGSASMPWVRLHAAKAYFDMAYL
                     LDKFPAIHATFNFTPSLVLQLQEIGAGTVHDLFLEHAQRRAASLTPEEQAFLIRHYFS
                     INWATMIRPYPRYHELLVKRGLNIDGQDLERLARQFTTQDLLDLQVWFNLAWFGYGAV
                     RRHPRLASLRAKNRGFTEEEKQEVLALQRTSIQEIVPMYRRLAERGQIELTTTPFFHP
                     ILPLIIDTEYTRRARPDLPLPSRFHAPEDAEAQLRRAVEFHTDAFGRPPVGLWPSEGS
                     VCPELIPMLPRVGLRWLATDEGILARSIEATQQSWHRQTQLYRPYRSGPAGQEVLIVF
                     RDREISDAFGFVYHKATAESGGEDLLRRIRQIVSDTPQDDILIPIILDGENPWEHYYE
                     GGERFLSHLYSAFANGVLDQEGSVRVTTATMSEALEAMPAKESLPHLHSGSWINQDFK
                     IWIGHQEDNRGWDLVGHTRARLVELTPTLHPDRAQAAWNELYAAEGSDWFWWYGDDFE
                     TAYKEEFDRLFRMHLRNVWTIAGEIPPETLSHPICEALRLPALDQVASPLSLLHPTLD
                     GLVTDFFEWRGAGTINPNPPLGAMWKAEGFFSAIYFGFDLEQLFLRLDPDDNLRGREK
                     DLRVECHLQTPTQAYRLSISLDTPDHYALAEHVGSGSWQETGSSQLIGWRKVLELALP
                     FKDIHVEAEQVLRMSVVVQSHGLEIARYPHHQPATLTVPGPGFEATVWRV"
     gene            11286..12290
                     /gene="galT"
                     /locus_tag="VLD60_02360"
     CDS             11286..12290
                     /gene="galT"
                     /locus_tag="VLD60_02360"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSB43821.1"
                     /translation="MPDLRRDPIVGRWVIISTERGGRPQDFAQMQQARPISTALCPFC
                     PGQERLTPKEIMAYRPQPSEANLPNWTVRVIPNKFPALQVEGELGREGLGLYDRMNGI
                     GAHEVIIESPEHNTGLGDLPPKKIEDVLWAYRDRILDLKKDVRFRYILIFKNHGAAAG
                     ATLEHSHSQLIALPVIPTSVMEEIDGCRAHYQQKERCIYCDILRQDLSDGDRVVAENP
                     EFVCVTPFAPRFPFEMWILPKRHAAYFQECQKQQFEFLAPILSESLRRMDKVLSKPAY
                     NFILHTSPLHEKTGDFYHWHVEIIPKLTQVAGFEWGTGFYINPVSPEESAKFLRDAEI
                     "
BASE COUNT         2833 a         3942 c         3652 g         2744 t
ORIGIN      
        1 gcagtaggac tgcaccgtcg aaatccccat cttggagaaa atcttcagca ggcccttatt
       61 gatcgccttg atgaacttgc cttccgccgt ctgcgcatcg agtccttccg gaagataatt
      121 gtcgcgctcc atatccacca aagactcgaa gacgaggtac ggattgatcg tcccggcgcc
      181 atagccgatc aagcaggcga actggtgcac gtcgcgcggc tcgccggttt ccagaatcaa
      241 accgacttcg gttctcgtgc actcgtgcac gagatgatgg tgaatagcgg agatgccgag
      301 caagctcgga atcggcgccc attcttcatt gacgccgcgg tcgctgagga tcaggaactt
      361 gtagccatcc ttgatcgcct gcgacgcctg cctgcacata tcgtccaccg cagcgccgag
      421 cccatccggc ccttcagcca cgcggaacaa catgcggagc gtcttgctct tgaagtgcgg
      481 atcggagatc ccacggatct tttgcaggtc ggcgttcgtg agaatcggct gctgcacttt
      541 gatgcggcgg caggactcgg gcgactcttc catcaaattg ggcttcggcc cgatgttcgt
      601 caccagcgac atgacgagct gttcgcgaat cggatcgatc ggtggattcg tcacctgcgc
      661 gaagagttgc ttgaagtact tgaacagcag ttgcgggcga tcggagagca ccgcgagcgg
      721 ggtatccgtg cccatcgacg agaccgcctc ttcgccggtc acgatcatcg gcgtgatgac
      781 cattttcagt tcttcgacag tgtagccgaa cgcctgttgc cgctggcgaa tcgtcggatg
      841 gtccggttgc gggacgttca acggctccgg cagctcatcc agcgagacac cgtattgcgt
      901 gacccagctg cgatagggtt tgcgaccgac gatatcggcc ttgatcattt cgtcatcgat
      961 gatgcggccc tgcaccgtat cgacaaggaa catgcggccc ggcatgaggc gccccttcat
     1021 acggatgtct ttggcttcgg ccggcaacac accggcttcg gaagccagca caaccatgcc
     1081 gtccgtcgtc acctgatagc gacaggggcg caaaccgttc cggtccagcg tcgcgccgat
     1141 catcttgccg tccgtgaaac agacagcggc cggcccgtcc cagggctcca tcatggcggc
     1201 gtgatattgg tagaacccgc ggcggtctaa atccatctgc gagttggcca cccagggctc
     1261 gggaatcagc atcatcattg catgcggcaa cgagcggccg cccaaaagga gaaactccaa
     1321 cgcgttgtcc agacaagccg aatcgctttg attctccgac acgatcggga agagcttttc
     1381 catgtcttgc ccgaacagat cggaatgaag ccggccctgg cgcgccttca tccaattgac
     1441 gttgcccttg agcgtgttga tctcaccgtt gtgacagacg tagcgatagg gatgggcgag
     1501 cggccaggtc ggaaacgtat tcgtgctgaa gcgtgaatgg accagcgcca gcgcgctgac
     1561 catgcttgaa tcggtgaggt cctgatagta ggccgccatc tgataaggca gcagcaggcc
     1621 tttgtagacg atcgtattgg ccgagaggct gggaatatag aaatactcgc ggtcttggat
     1681 ggccgattcc ttgatcgcct tctcgactct cttgcgaatg acgtagagtt tgcgctcgaa
     1741 ctgcgcttcg ttcaagacgt cgcgggcgat aaaaacctgg cgcatgaacg gttcggtctt
     1801 gcgcgcttgc accccgatcg cgtcgctctt gaccggcacg tcgcgccaac cgagcaggcg
     1861 tgcgccttca tcctcgataa tcttgccgaa cagtttctcg cattgctgcc gcgccgactg
     1921 gttcggaggg aggaacaact gcccgacgcc gtactcgccg gcatccggaa gtatgacccc
     1981 agcgtcgccc gctgcccgct tcaagaactc gtgggggacc tgaagcagaa tgccggcgcc
     2041 gtcgccggtg caggggtcgc atccttgcgc gccacggtgg gtcaaattct caagaacttg
     2101 aagcccctgc cggacgatcc ggtgcgactt ctgcccttta atattgacga caaacccgat
     2161 gccgcacgag tctttttcgt gctgagggtc gtagaggcct tgcttcggtg gaagccctgg
     2221 gagttccatc atacgtatct cgtgaaaaag gcaggtcttt tcctgctgga cagaggccct
     2281 actgagagga atcagggacg cggaatccca ttactcttga ggcgatatcc gtttcaaaat
     2341 ggttgctcag attaatggat gggtgaatct cctgtcaagc cgaggagata gccgtttttc
     2401 aaagccgttc ccttgacttt gtttcctgct ctgccctacc atccgccgca tgccacaggg
     2461 tcctgttacc tcaaccattc acagcatggc ggacgtgtgg gacgcttacc gccatgagct
     2521 ggatggtgtc gaggaccagg tccgcagaaa tctgaactcc agcgtcaccc tcgtcaacac
     2581 cgtcgccgcc catattctga acagcggcgg caagcgtatc agacccctgc ttctgctcct
     2641 ctccgcccgt ctctgcggct atggtggccg cgatcatcac caactgggca gtctcgtcga
     2701 gttcattcat accgcgacgc tgctgcacga tgatgtcgtg gacgatgccg acatccgccg
     2761 gggccgccga acagctcgga aagtttgggg aaatcagatc agcatcctgg tgggcgacta
     2821 cctctattcg aaagccatgt gtcaagtggt ggagttccgg agtcagggta ttaatgaagt
     2881 gctggccgaa gcctgcaaga agatggcgga aggcgaagtc ctccaattgt attacaacgg
     2941 caatccggcc atgccggaga ctgactacct cacgatcgtc gaacataaga cggctggcct
     3001 gatcgccgcc tcctgtcgca tgggtgcaat tctggcggag gcttcggagg tgcagcagga
     3061 tgccttgttc cgctttggtc aatatctcgg gatcgccttc caagtcgccg acgacacatt
     3121 ggattacacc gccaacgggg aatcattggg gaaaacactc ggccaggatc ttcggcaagg
     3181 aaaggccacg ctgccgctcc tgcatctgct caatcactgc tcagaggccg atcggcagat
     3241 gatcaaggac cggatggaaa ccagaacctt gacggaagag gatttgagcc ggctcatccg
     3301 cttgatggaa gaatacggtt ccatcaacta tgcgctggga cgggctcagg cttatatcgc
     3361 cgccgccaag cgggatttgg attcatttga agacaatacg gccaagcgag ccctctcggt
     3421 ggctgcagac tacatggtga cccgcgaccg gtagccccgt tttccgcgcg gtcccaggcg
     3481 gtttacgcat gggacgggca acgcgcagga tgaacggccc agcctttcgg ttcttgtcta
     3541 cacatatata tttcactcac ggagaaaagg atcacgcatg gcacagatca ttccgtttcg
     3601 aggcaccttg tacaatccaa ctgtggttgg cgctgtccgc gatgtcgtcg cgcctcctta
     3661 cgacattatc gatgcggcag gccagaaggc cttgcacgat cgtcaccccc aaaacatcat
     3721 ccggctggaa ctcggcctcg atcagccagg ggacaatccg aggaataacc ggtacaccag
     3781 agccgccgat atgctccatg actggctcaa gagcggtgcc ctgaagcgtg acgcccaacc
     3841 ggctatttac tatcacacga tcgaatacgc tccgcctgcc tccaagcccg gcgcgccgaa
     3901 gaaagtcctg agaggatttc tcgtcacgac caagttggaa gcgttggact cgggacatat
     3961 ctacccgcat gagaataccc gggccgctgc gaaaacggac cgactgaatc ttcttcaagc
     4021 ctgcaaagcc aatctgagtc cgatttggct cctctactcc gatccgcaga acgccatctt
     4081 aagcctgctc gaaaacgcca cgaaaggaac gccggcccgc atcgattttc aggacgacag
     4141 cagctgccac cagcgactct gggtggtcac ggatccggcc gttctcaaac agatcgtcga
     4201 gatcatgcag aacaaaccgc tcttcatcgc cgacggacat catcgctatg aaacagccct
     4261 gaactaccaa cgcctgcgga ggcagcaagc aggatcaccg gacgggcttc aaccctatga
     4321 tggcgttctg atgctcctcg caccgttgga ggacccgggt ctgaccgtgc ttcccactca
     4381 ccgcgtgacg accacaccgc tacctcccta tcaaaaggtc aaggcgctga tgggtgagaa
     4441 attcgaaata caagagttcc ccttcaccgc gaacacgcag gcggcagtcc gagcccaatt
     4501 catcgaggcc ctgcgcacaa acggacagac cacgcccacg tttggtctgg cgatcaaagg
     4561 ggaaagccgc tacgtcacgc tggcgctgaa ggctgctcat cgccccacgg ctcaggactc
     4621 cccccgcgcc aagctcgacg tctcattgtt acaacaattg gtggtggccg ttctgtgtcc
     4681 gacgcaagag gagcaggaag ccattcttta tactaaggac gaccatgaag cgcttgactg
     4741 ggtcgccaac gggactggga ccggagcgtt gctcctcaat gcgaccaaag tcagcgaggt
     4801 gcaggcagtg gccacagccg gtgaacggat gccgcacaag tcgacctatt tctatcccaa
     4861 accgctcacg ggactggtga tcaatgtcat ggaagaatag ccggcgtgag gcgtaagggc
     4921 tgaggggtaa ggcgacacgg tgatattccg ggcgggcatg aagcgattct tctatcgcct
     4981 cacgcctgac gcctaacgga gggatcatga aagcgaagat tctcatcgtc gacgacgatc
     5041 cggacattgc gaccatgttg gaggatcggc ttcaagcctc cgattatgga acggtcacag
     5101 cacgcgacgg cgtccaggcc cttgagatgg tcgagcagga agcgccgcat ctcatgttgc
     5161 tcgatctgga catgccccgt ttgaccggac ttgaagtcct caaacgtctg ccgaaggtca
     5221 ggcccgccga agatctccct gtcatcgtca tgaccgccca cgggtcaatc gaagccgccg
     5281 tcgaagccat gaaagacgga gcctacgatt ttctcaccaa accgctggac aaagatcatc
     5341 tcttgatcgt gatcgacaaa gcgttggaac gcgactcgtt gaaacggcag gtcgcctgcc
     5401 tcaagtccga ggtcgatggc cgctacgctt ccatcgtcgg caccagtgcg aaaatccgca
     5461 cggttttgga tgcggcgcag cgcgccgcca aatcggacgc cagcgtcctg ctgctaggcg
     5521 aaagcggaac gggcaaggag cttctggctc gctcgattca tcaatggagc ccgcgccatg
     5581 cgatgccgct cgtggtcatc aattgcgtgg ccttgacgga aaccttgctc gaaaatgaat
     5641 tgttcggcca cgaacgcggc gcgtttaccg gcgccgatcg gttacaaaag ggcaagttgg
     5701 aaatggcgga cggcgggacg gtgttcttgg acgagatcgg cgacatgtcg ctcccgctcc
     5761 aatccaagct gttacgggtg ttgcaggatc gtgaatttca tcgtgtcggc ggcacccggc
     5821 tcgtctccgt caacatccgt gtcatcgccg ccaccaataa agaactgaag caagcggtca
     5881 aggccgggga ttttcgcgaa gacttgtttt tccgcctgaa tgtcatcagc ctgacgcttc
     5941 cccccctgcg cgagcggctg gaggacttcc ccgcgttggc caaattcttc ttgaaccggc
     6001 atgccagaga ggcgaaacga cccggaatga tgttcagtcc tgcctcgatg gatgccctgg
     6061 ctcactacac ctggccgggg aatattcgcg aactggacaa cgtgatcgca cgcgcggtca
     6121 ttctgagcca caccgatacc atcgagccgg atgtcctggc actcgacttc aactctgcca
     6181 ccgggaaaag tgacgctcta ccttacgtca tgttgcccta ccacgaatcg atggacgccc
     6241 atagtcgcca catcatcgag caggcgctca aacaggcgga gggcaatcag accaaagctg
     6301 cagaacgttt acgcctgcaa cgaacctacc tggctcgctt gatcaaacaa aagcagtcca
     6361 agttcattta agcggggatg ctcttacacg cgggtctgcc gggctttgtc agtcgatgca
     6421 agtctgcact tgataaggaa ggtgatttgg cgggccataa gctcgccaag gggttctcgg
     6481 agactgttgt cagttcatag ttggaccggt tgccgggctt gtagagaaat cagtcccgca
     6541 ttcgcatcat gttcggcctc agcatagcgt tgagctgtcc cgatatcgga ccagtaaccc
     6601 tccaggtcat agcccaggac aacctcgtcc cgttgaatcg ctgacacata cgcatcgatg
     6661 atggacgact ccttgccctt cggcacgtcg cgcaagaggc gagggtgtag gatatgaatg
     6721 ccggcgaaca tccggggttg ggttggaccg gcttccgatt ttccccggcc ggtaatacga
     6781 acgatccgtt cgtccgggcc cacctcgacc aacccccaac gcgcagcctc ggcgtccgat
     6841 cttaacacga gcgtcgcggc agccttgcgc tggtggtgaa acgcgcttaa ggcctcaagg
     6901 tccaattcaa agagtgtatc gccgttgagc accaacaccg gttctcctga aaaattcggt
     6961 tctgcctgtt tgatcccgcc gccggtcccc aaaatcaccg gctcatgtga atagtagatc
     7021 cgcaggccgt atttcgaccc gttgccaagg gcctgttcga tcatcggccc cagatggtgg
     7081 agattgatca cgacgtcgtg aaacccgtgc cgcttcagga gcaaaagatt ccacacaata
     7141 agcggagtgc ctgcgatagg gagcagcggc ttggggatcg tattggtcag cggtctgagg
     7201 cgcgtgccca gacccgccgc caggatcatg gccttcatga tgcctcgtga ggcgtgaggg
     7261 gtaaggcgtg aggggtttct gctccccttc gcctgacgcc tgacgcctga cgcctgacag
     7321 ttttactgca attccggcac ataggcaccc atgagtttcc gtaacgtgaa gagctccgga
     7381 tacttgtgca gattccgttt cacatagccc aatgtccgcg ggatgtcggc tagaaattta
     7441 gggttgcctt tcacgcgatc gatatagacg aatcgaccag cagctttgag attgcgttga
     7501 atgctcgtga ggtcgaacag acggcgaaag gcctctcggt tcgtccagac ctgacgcccc
     7561 gactccagcc gatcgagata gtaatcgatg agacgatcga tcagcgcctc atcaagcgca
     7621 atataggcat cgcgcaagag cgaggccagg tcatatgtgg ccggccccat caacgcgtcc
     7681 tggaaatcga tgacccccag ccgatgtcca tccaccatga gatttctgga atggtaatcc
     7741 cggtggacga atacccgtgg ctgtccggca agcagctcgg agattttctg gaattcgtcg
     7801 cgcatcacag cctggtcgtc cccgctcatc ggcttttcct gccgcgccac gataccgtac
     7861 tccaaaaaat gatcgaattc ccacatcaac aacggcacat caaaacttcg attgaacgcc
     7921 aggcagcctg gatcagctgg agaggtggct ttcgattgta attggatgag gatgtcgacc
     7981 gccagcttgt agcgagcctc aagttcggtc atcgttgcat cacggcaggc atctgaaagc
     8041 gtgacatcgc caaaatcttc gaggtacagc aatcctgcaa cttgatcgta gaaatacaga
     8101 ctcggcacgg gaaccccggc cttcgccaga tgtgcctgga tattcaggaa gggcaagtcg
     8161 ttgatctggt gagccgctcc gctgaccgct tcttccgatt gcttgaacgc ctccggttca
     8221 gccaactgca tcaggatgac gggccgagcg gtctgctcgc tgagttcgat ccggaaatag
     8281 cgccgattgg aggcatcccc ggcaagggcc gtgagcttct tcaccgtccc gcgaaaggga
     8341 agcttggtcc gtaccgtctc cgcaacgagg gcccgatcgg gaagggcaag cggttttttc
     8401 ggcgcggcag gatcgatggt ggtcatcagc ggcgcattat actgttgtgt ccacaccttg
     8461 tcacggcagg tcggcccgcg tatcccgctc cgtgaagcgg cgcaatccca ccgcgccgat
     8521 caccccgccc atcgtatccg ccaaccaatc cagccagctg gactcacgga atggaacaaa
     8581 ggcttgatgc acttcatccg tcatcccata gaacgaaacc gccatgatcg tgatgatcac
     8641 ggcgcgacgg gccactgatg gtccggtcgc ccagcggagt gcccggtagc acaacaatcc
     8701 aagcacggca tactcgacaa gatgcaggaa tttatcgctc agcttcttga acacaaactc
     8761 cggcagttca tcttcggggt gcggcagaga agagaggtag aaaatcagca tggcatagag
     8821 cagtaccggc aaccagtacc acaagatcgg atagcgagag ctgacacccg tcgcagacaa
     8881 aaccttttcc atttatcccc gttgattgca agccctgtat ccctatgaca tactcttttt
     8941 cagtccgcaa tattcttcgc acatcgtcta tgaaaacggc tcgcgtctgc tttctgtggc
     9001 acatgcatca gccctactac accgacccgg taaccggttc ggccagcatg ccctgggtac
     9061 gtctccatgc agccaaagcc tattttgaca tggcctatct cttggacaag ttccccgcca
     9121 tccatgccac gttcaacttt accccctcgt tagtcctcca gctgcaggaa atcggggccg
     9181 gaaccgttca tgatctcttt ctcgagcatg cccagcggcg tgccgcttcc ctcactccag
     9241 aagagcaggc ctttttgatt cgtcattact tttcgatcaa ttgggcgacg atgattcgcc
     9301 cctacccccg ttatcatgaa ctgctggtga aacgggggct gaacatcgac ggacaagacc
     9361 tcgaacgtct cgcgcgacag ttcaccacgc aagacctgct cgacctccaa gtctggttca
     9421 acctcgcctg gtttgggtat ggggcggttc gccggcatcc gcggttagct tcgttgcggg
     9481 ccaagaaccg gggatttacc gaagaggaaa agcaggaagt gctggcgctt caacgaacgt
     9541 cgatccagga gatcgttccc atgtatcgcc gattggcgga gcggggccag atcgagttga
     9601 cgacgacgcc gttcttccac ccgattcttc ccctcatcat cgatacggaa tacacgcgcc
     9661 gcgcccgacc ggatctcccg ctgccttccc gttttcacgc cccggaagat gctgaagcac
     9721 aactgcggcg cgccgtggaa tttcatacag acgcattcgg ccgcccccca gtaggactct
     9781 ggccatcgga gggatccgtg tgccccgagt tgattcccat gctcccccgc gtgggtcttc
     9841 gttggctcgc cactgacgag ggcatcctcg cacgttcaat cgaggcgaca cagcagtcct
     9901 ggcatcgaca gactcaactc tatcggccct atcgctcggg acctgcggga caggaagttc
     9961 tgatcgtatt tcgcgaccgg gagatttccg atgctttcgg ttttgtgtac cacaaagcca
    10021 cggctgaatc cggtggggag gatctcttac ggcgcatcag gcagatcgtg tcggacacgc
    10081 cgcaggacga catcctcatt cccattatct tggatggcga gaatccttgg gagcattact
    10141 atgaaggggg cgagcggttt ctctcgcacc tctactcggc ctttgctaat ggggtattgg
    10201 atcaagaggg atccgtccgg gtcacgaccg ccacgatgtc cgaggcgctc gaagccatgc
    10261 cggccaagga gtccttgcct catctgcatt cgggctcatg gatcaatcag gatttcaaga
    10321 tttggatcgg ccaccaggaa gataaccggg ggtgggatct cgtcggccac acgcgggccc
    10381 gccttgtgga gttaaccccg acattgcacc ccgatcgagc ccaagcggcg tggaacgaac
    10441 tctacgcggc ggaagggagc gattggtttt ggtggtacgg cgacgacttc gagacggcgt
    10501 ataaagaaga attcgaccgg ctctttcgca tgcacctgcg caacgtttgg acgatcgccg
    10561 gcgagatacc tcctgagaca ctcagtcacc ccatctgtga agcactgagg ctccccgctc
    10621 ttgaccaggt cgcgtcaccc ctctcactct tgcatcccac gctcgacgga ttggtcacag
    10681 atttttttga atggcgcgga gccggcacca tcaatcccaa cccgccgctc ggagcgatgt
    10741 ggaaagcgga aggtttcttc tcagcgatct acttcgggtt cgatctcgag cagctgtttc
    10801 tccgcctcga ccccgacgac aacttgcggg gtcgggagaa ggatcttcgc gtcgaatgcc
    10861 atctccagac cccgactcag gcctatcggt tgtcgatctc cctggacact cctgatcact
    10921 atgccctggc ggagcacgtc ggcagtggta gttggcagga aaccggttcc tcgcaactga
    10981 tcggctggcg caaggtgctg gaactcgcgc tccccttcaa agatattcat gttgaggcag
    11041 agcaggttct gcggatgagc gtcgttgtgc aaagccatgg attggaaatc gcccgttacc
    11101 cccaccatca gccggccacg ctgacggtgc cggggccagg attcgaagcg acggtgtggc
    11161 gagtgtgact ggggcagttc tgagtgttgg gtgctgcgtt atgtgttgtg aaaatccgat
    11221 gtgtctgaga gactcattac tcagcactca gggctcagta ctgttttaga ctatgcggag
    11281 caccgatgcc cgatctcaga cgtgacccca tcgtcggccg ctgggtaatt atttccacag
    11341 agcgaggcgg ccggccgcag gactttgcac aaatgcaaca agcccgtccg atctctacgg
    11401 cactgtgccc cttctgcccg ggccaggaac ggctcacccc gaaagagatc atggcctacc
    11461 gtcctcaacc ctcggaagcg aacctgccga actggaccgt ccgggtcatc ccgaataagt
    11521 ttcccgcctt gcaagtcgag ggagagctgg gccgcgaagg gctcggcctc tacgatcgga
    11581 tgaacggcat cggggcgcac gaggtcatca tcgaatcccc ggagcacaat accggcttgg
    11641 gggacctgcc gcccaagaaa atcgaagacg tcctgtgggc ctaccgcgat cgcatcctcg
    11701 acctgaagaa agacgttcgt ttccggtaca tcttgatttt caagaaccat ggagccgcgg
    11761 ccggcgccac cctcgaacat agccattcgc aattgatcgc cttgccggtc attccgacca
    11821 gcgtgatgga agagatcgac ggctgccggg ctcattacca gcagaaagaa cggtgcatct
    11881 attgcgatat cctgcgacag gacctctcgg atggcgaccg cgtcgtggcg gagaatccgg
    11941 aatttgtgtg cgtgacgccc ttcgcacctc gattcccctt cgagatgtgg atcctcccca
    12001 agcgccatgc cgcgtatttt caggaatgcc agaaacaaca gttcgagttc ctggctccga
    12061 ttctctctga atcgttacga cgcatggaca aggtgctctc caagccggcc tacaacttca
    12121 ttctccatac ttctcccctc cacgaaaaaa ccggcgactt ctaccactgg cacgtggaga
    12181 ttattcccaa gctcacccaa gttgccggct ttgaatgggg caccgggttc tacatcaatc
    12241 ccgtttcgcc ggaggaatca gcaaagtttc tccgcgatgc agaaatctaa ccggtttcgg
    12301 tccttccggc tgttgttcac ctgtttatct cccgacagag cgacgacggc ctcttcctct
    12361 cgatgaattt gcggaccagc gacgcagcat gacatgaccg tctccctgca gtttatgcag
    12421 ggaggtgtcc ggccatggct tagacgatcc atcctgcaag ccaagggttt tgtatcggat
    12481 tcaaagacat gaccgcggta acggacgcaa tgtttgcaca agcgtgatgg gttgtccctg
    12541 cctctgatac ctcattctca aattcctgcc gcctaaacgg tcgctccatt tggaaaaggt
    12601 cttttccggt cctcgtccga agatttcggc atgtctgttg cttcgtgaag tttctggatg
    12661 acgagcgctc atattgcttc gagttggaga ttcacggatg cctcctctca cctgtgcgaa
    12721 atgccgaaag gatattacca gcgccctttg ggataaccag acgagccagt tttactgctc
    12781 tcagtgcgtt ccaccgttac caagagtcac tcctccagaa gcacagagca agaaagggcc
    12841 acgaccaggg atgatgaccc aaccaaaggg ccgacgacca acgtagtcgt tcgatgaatc
    12901 aaccggaaca tcaacactta gagcctcccg attatccctg tgaacgatgc ggcaactcca
    12961 agccacgact cgcctttgac cagctgagcc ggctccttct ctgtgaatcc tgcttggatc
    13021 gcgtgcaacc acagcagtcc tggtccttac tgcgcgaggc tgacaatgtg tgacagggcc
    13081 ggatggaaca agcagatcac ccgctcgaat ccgtcctctc gagctcccct gacggaggaa
    13141 taaaacaagc tcagttcgtt catggctcgt c
//
LOCUS       DATIFR010000047        17353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1943639, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000047 DATIFR010000000
VERSION     DATIFR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17353
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943639"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VLD60_02365"
     CDS             <1..201
                     /locus_tag="VLD60_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB43822.1"
                     /translation="VAPGFIDTAMTQGLSAEVKDALQKQIPLGRLGTPADIANAVRFL
                     VSEEAAYITGHVLHVNGGMLMS"
     gene            294..527
                     /gene="acpP"
                     /locus_tag="VLD60_02370"
     CDS             294..527
                     /gene="acpP"
                     /locus_tag="VLD60_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSB43823.1"
                     /translation="MATVDERVKKIIAEQLGVEEDEVTPEASFVEDLGADSLDTVELV
                     MALEEEFGIEIPDEDAEKILTVGKALDYIKEKA"
     gene            543..1793
                     /gene="fabF"
                     /locus_tag="VLD60_02375"
     CDS             543..1793
                     /gene="fabF"
                     /locus_tag="VLD60_02375"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSB43824.1"
                     /translation="MSDRTTRRIVVTGLGVITPLGTGVEKTWKAICAGESGIGRITKF
                     DPTGYDAQIAGEVKDFDPARFIEKKEIKKMDPFIHYAVGAAQLATDDAGLKVAPEEAT
                     KVGVYIGSGIGGLGSIEHYHNVLKEKGPGRVSPFFIPMTIINLASGQVAIRLGAKGPN
                     SCAVTACATGNHCIGDAFRLIQRGDADVMVAGGAEAAITTLGVAGFAASKALSFRNDE
                     PTKASRPFDRDRDGFVLGEGAGVVVLEELEHARRRGVRIYAEVIGYGMNSDAYHITAP
                     SEEGEGAVRCMELALKDAGIGKDLVGYINAHGTSTMADVIETKAIKQVFGERAYRIPV
                     SSTKSMTGHLLGAAGGIEAVFSILALHHGILPPTINLDNPDPACDLDYVPHKARPASI
                     NVALSNSFGFGGVNACLLFKKPEA"
     gene            1821..2528
                     /gene="rnc"
                     /locus_tag="VLD60_02380"
     CDS             1821..2528
                     /gene="rnc"
                     /locus_tag="VLD60_02380"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSB43825.1"
                     /translation="MTSASSVDALQSSLAYRFQNLALLEEALTHKSHVNEQKTPAGLH
                     NERLEFLGDAVLSLLVSERLVALLPQSSEGALSKLKARLVSETVLAEVARRLKLGSCL
                     RLGRGEELSKGRDKDSLLADALEAVIAAVYLDGGLDAGRQVVIRLFAEEFAEVATQQH
                     RPGSEDYKTQFQEWCQKRFDGLPQYAVVRESGPDHDKLFEVELRFKGDVVGRGVGHSK
                     KEAEQLAARQALQQADA"
     gene            2589..3176
                     /locus_tag="VLD60_02385"
     CDS             2589..3176
                     /locus_tag="VLD60_02385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB43826.1"
                     /translation="MQRRRTVIVIAVSAILSGALLWGVGLPFAADKAPAKSPRAVIKT
                     KFGDMEVTFFPEKAPKHVENFITLARSGFYNGTIFHRVIPGFMIQGGDPNTKDPNKPE
                     TYGMGGPSHRLKAEFNDIPHRRGVLSMARTNDPNSAGSQFFIVVKDSNFLDGQYTVFG
                     EVVKGMDVADTIVNLPRNARDLPAERAEMTVVVLE"
     gene            3213..3821
                     /locus_tag="VLD60_02390"
     CDS             3213..3821
                     /locus_tag="VLD60_02390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB43827.1"
                     /translation="MAMRGFALFLACAVLSAVAACSARSEVKTTLPPPSDPHPRAIIT
                     TKFGAIEIKLLPELAPNHVENFIKLAKSDFYNGTIFHRVIPGFMIQGGDPNTKNSLRK
                     ETFGMGGPKDEKGNPILLKAEFTDTPHKRGIVSMARGNEPDTAGSQFFIVVEDSHFLD
                     RKYTVFGEVTKGLGVADKIVNLPRDERDLPNERVEMKIIIVE"
     gene            complement(3841..4164)
                     /locus_tag="VLD60_02395"
     CDS             complement(3841..4164)
                     /locus_tag="VLD60_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB43828.1"
                     /translation="MAVEREKKIRKLRRVDLQCQLGFSSGDIEGDARVTNISTGGCRA
                     ESEINLAEGLEFQVLLQLPGELSPVKIERAAVRWVTGDIFGMSFILFLPSERARLKAY
                     LDTFK"
     gene            complement(4188..4889)
                     /locus_tag="VLD60_02400"
     CDS             complement(4188..4889)
                     /locus_tag="VLD60_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43829.1"
                     /translation="MSQCAYCSQRKGKRPCPALGGLICSQCCGEHRIVRVSCPSDCVY
                     LGTGSHYQQKRLSEQFMPMRREFYQELGELGGEKAVALYNLIEVVTFSYFHGRRDGQD
                     AEVVAAVQALRRTLSPLHVPSAPMPVFAEHLKKEYDSFKKQNPQQIADASTAPDVLDR
                     AIKFILAFSGTDFQSQRFLSGLVGYVRTYHPEIAAHLTKQHEPGHIVLPGQSFMPPAA
                     PEPHTHGPGCEHHHH"
     gene            complement(4886..5908)
                     /locus_tag="VLD60_02405"
     CDS             complement(4886..5908)
                     /locus_tag="VLD60_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43830.1"
                     /translation="MNKLAAPYFNMTHTIRHSIACCAGLVLYALAPLAEAQITVTELQ
                     SPYSFVTGQWGKEYFISSVNGEPETADNNGFITKLDANGKLLDLKFIQGGKADVTLHA
                     PKGMALVDQTLYVADLDQIRAFDIYSGKSVATIPIAGAATQTPAHLTDVASDGKGLLY
                     CSDQKANRIYRVELATQKVSILITDPHLAGPTGLAVHPKSGQLIAVSWDKGKIFEISP
                     EGAMSELFSNGFFSSRFANLRGVDFDRWGNMYVSDFTTGKVWRMTRDKRFQVIAEYLP
                     SPGDISIDRTNNLILVPYEFAHAAEMNGLEAPHDGKPKQEKRTLADYGFIPPPPKPAT
                     EGAASK"
     gene            6188..7048
                     /gene="folD"
                     /locus_tag="VLD60_02410"
     CDS             6188..7048
                     /gene="folD"
                     /locus_tag="VLD60_02410"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HSB43831.1"
                     /translation="MAARLIDGKALAQQVRERLAGESAAVLAKTGMKPGLATILVGDD
                     PASHVYVKNKQKACELAGIYVDDHKLSASTTQAELLALIEKKNADPKIHGILVQLPLP
                     KHIDSRVILEAVSPNKDADGFHPYNFGRLVEGHPVFEACTPKGVIKMIESAGVSIEGK
                     RAVVLGRSNIVGKPLALMLLQRNATVTICHSKTKDLPAVCREAELLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKLPDGKLCGDVDFEAVSQKAGWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGML"
     gene            7165..8055
                     /locus_tag="VLD60_02415"
     CDS             7165..8055
                     /locus_tag="VLD60_02415"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HSB43832.1"
                     /translation="MSREPKRLREVLDQGQFAVTIEYNPPKGTNISGVLDSARQLVGR
                     VHGVNVTDNTAAVVRAGSLPVCRLLYELGHDPVMQMTCRDRNRIAMQSDLMGAHMLGI
                     RNILCLTGDYPTVGDHKDAKPVYDLDSVQVMQLVQGLNNGRDMAGNKLDGSTAFNIGA
                     AVTPEADPVGPMLAKFEVKVKAGAQFFQTQAIYNTEQFARFMKAVRPFKVKVLAGILL
                     LRNAKMAEFMNANIPGMSVPQEMIDELRAAGDKSEDVGVEIAVRTINAVRPHCDGVHI
                     MAIKGIHRLGEIITKAQLGS"
     gene            8052..8840
                     /gene="panB"
                     /locus_tag="VLD60_02420"
     CDS             8052..8840
                     /gene="panB"
                     /locus_tag="VLD60_02420"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSB43833.1"
                     /translation="MTVPDLLRYKREKKKVIVVTAYDALFTRIVEQAGIEAILVGDSL
                     GVVVQGKANTLSVTMEDMVYHTRLVAGAAQRAMVIGDMPFMSYQASKEDALRNAGRFL
                     QAGAHAVKLEGGAVVLDRVEAMTQLGIPVIGHLGMTPQSVHQYGGYKVQGKGKDQAET
                     LLSDAKALEAAGAVAVVLEAIPAELAKAISEQVAIPTIGIGAGSDCDGQVLVLYDLLG
                     MFDEFVPKFVKPYAHLKADALQALRRYKEEVEQGKFPSDSESYH"
     gene            complement(8860..9375)
                     /locus_tag="VLD60_02425"
     CDS             complement(8860..9375)
                     /locus_tag="VLD60_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HSB43834.1"
                     /translation="MTEPQPPDPSWPRYSARTFPSYRFVPGNNQHPRRKPEGHSQGFG
                     EPDPTPFAPEQWRESDDYCYGVDLYNYAYWWECHEVFEALWHVAGRQSDQGNFFQGLI
                     QLAAANLKLFMGNHQAAQNLLHSGLVRLQRVPEFYMGLDVAGFTNDLRQCIMRSYEQA
                     PPIRLAMSIGK"
     gene            complement(9368..10183)
                     /locus_tag="VLD60_02430"
     CDS             complement(9368..10183)
                     /locus_tag="VLD60_02430"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HSB43835.1"
                     /translation="MATYAIGDIQGCFVSLQQLLERISFAPKTDKLWLAGDLVNRGPD
                     SLATLRFIKNLGASVRLVLGNHDLFLLAVAEGIAALRPKDTIRDILSAEDRSDLLEWL
                     RRQPFHHREGSFLVVHAGLLPQWTVEEATALAQEVETALHGPGYRTFLKALFHGPTPH
                     WNSSLKGSERLVSIARVLTRLRTCTSTGEMSNFSGPPDEAPAGFTPWFRILDRRNRGT
                     TIISGHWAALGLHMEPELLAIDSGCVWGRQLTAIRLEDRSVFQVDYADKPRRD"
     gene            complement(10229..10642)
                     /gene="queF"
                     /locus_tag="VLD60_02435"
     CDS             complement(10229..10642)
                     /gene="queF"
                     /locus_tag="VLD60_02435"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HSB43836.1"
                     /translation="MRGKGSTRQRTTKLGYNERHAKSGIASPLPDIETFPNQYKGYEI
                     TIEIPEYTAICPKTGLPDFGTITVHYMPDKECLELKSLKMYIHAYRNIGIFYENSVNR
                     ILQDIAKACHPVWANVTGTFAARGGLRSVIEARYP"
     gene            complement(10665..10913)
                     /locus_tag="VLD60_02440"
     CDS             complement(10665..10913)
                     /locus_tag="VLD60_02440"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HSB43837.1"
                     /translation="MELRVLIAGEDEGAQHVGGVHKRPPIPDDSLKAECPKFMSHGPC
                     GGVRKGGLCEVYPEMKCPWVSLFIELEKIGHTEWMKQV"
     gene            11330..12232
                     /locus_tag="VLD60_02445"
     CDS             11330..12232
                     /locus_tag="VLD60_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSB43838.1"
                     /translation="MSLDYVKFSNGFEKFMPKEYRDMVEHGPFGKKISVSQMGSFKEI
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLTRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMVQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKTVENDRYAFSEYISDEAKEYL
                     TERYGYKEFLPKPAAPAAAIPGKA"
     gene            12256..12978
                     /locus_tag="VLD60_02450"
     CDS             12256..12978
                     /locus_tag="VLD60_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSB43839.1"
                     /translation="MAKRFNIRMAGVGGQGVVTGSHILSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKEDGIAL
                     INNDGPMNLHKDQARELEERRAKLYYFPATKISLEVAGMDLATNMALMGCIGAITGLT
                     NMLGLEQAVKDRFLGKGFVVSGGTAALDSVVERKFKKKAELIEKNVAVMRAGWNYAVD
                     HGWAAPEVKRAEEPVATATATA"
     gene            12989..13312
                     /locus_tag="VLD60_02455"
     CDS             12989..13312
                     /locus_tag="VLD60_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HSB43840.1"
                     /translation="MYLVADINVEICASKSCKLCTQYCPEANTILYSEEMGREKGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPKAALTDNITYGEKSTTAVLA
                     SPVVG"
     gene            13328..14098
                     /locus_tag="VLD60_02460"
     CDS             13328..14098
                     /locus_tag="VLD60_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HSB43841.1"
                     /translation="MGVGTTQDTRERIIVPGPAGFHPPSAAQLGVALPDPGEGLFYGL
                     LEPNEEKVIEEMARKMLTSPNATIFPGPLLLWAWNEHAVEKAKATLEIAAQIPEVMII
                     PMPDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNC
                     CTMAVCAEQGHEDAMLTIRDSDTAKIKRVAQIFKKVREEMGIKLPENGENVRFTGTQS
                     KVHGGKTHTNPMAFAPSPAGAGSAAAFGHSPEQMKREG"
     gene            14138..15481
                     /locus_tag="VLD60_02465"
     CDS             14138..15481
                     /locus_tag="VLD60_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSB43842.1"
                     /translation="MSEALEAQQKTNPQGDPITSVAAPKLDGKKDPHADAKKQRIVTP
                     EYMFQEAPRTKEFITGSEAAKEAIRRSNVDLAIAYPITPQSETMQLVGVLYGEGYVKE
                     YYRGEEEVGVMAAIAGGSRSGVRCYTATAGPGTLRGLEGIASWPGHRLPVVAMFTCRV
                     VNAPLAIQPDNIEVAYLLNCGMIVFHAENQQDMFDFTMAGFVISEKNDVTLPVGVCCD
                     GFFVTHARGYVRMQERGIKLPPREAWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNI
                     HAVWQQEVWAAVERSRKYINRYMGGLLTAENVEDAEVLIIASGSAAAQSREAVRLCKE
                     KGMKVGLIKIRSLRPFPTKELRQLCGKAKLIVIPEFNYVGWLAKEVATAIYGFSKAKV
                     IGGPRVYGGQSMPVELIVDEVESGVSGKKSTNVAMSSIMGGAVNPDEVAHFMRSI"
     gene            complement(15628..15876)
                     /locus_tag="VLD60_02470"
     CDS             complement(15628..15876)
                     /locus_tag="VLD60_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43843.1"
                     /translation="MTDAILQAYKEVELAIERYTTLLQDHVTTLQNMEPPGSDKVVRM
                     THGSKAMRDSALIYLSYAKYVAYGMPASEEMVEDEIQG"
     gene            15947..17134
                     /locus_tag="VLD60_02475"
     CDS             15947..17134
                     /locus_tag="VLD60_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HSB43844.1"
                     /translation="MPPLLLRALERLNDVESLPGSSGYAEAVAELSRLFTKERVALRS
                     GYLDDPDHAAAYLRYFLPVNLSKIQVLLDEMPEATLIDKQGSGLNVLDLGSGPGTGAL
                     AVLDWLHQRHPEQAGKLTVVAIDSSADALSQAGQLWDSYCRESGIAGARLTPYETDLE
                     RPLNGPSGGQIYKNGPYDVIIMANCLNELFSRAVDPIGARTGLLAGLLTLLAPHGTMM
                     LVEPALRTTSRELHEVRDRLLHDRRCTVYSPCLHERPCPALVNPDDWCHEERAWDPPA
                     AIQAIDQEVGFIKDALKFSYLLLRTDGRMIVERRPDVYRVVSELRELKGEKRAWLCNE
                     LGRQEVGRQDRLASPENAAVDAWHRGAIVQIERIVRKEMGGKVSALGRIEQDATVKIV
                     RPV"
     gene            complement(17121..>17353)
                     /locus_tag="VLD60_02480"
     CDS             complement(17121..>17353)
                     /locus_tag="VLD60_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43845.1"
                     /translation="AGTGPLAVCPRPIFYPGTAGLIPNVRASNELRQTQLVRPGAIIT
                     DPGDGIKPMCRAALHEHKGLALPALFKPFIRV"
BASE COUNT         3825 a         4561 c         5337 g         3630 t
ORIGIN      
        1 gtggcgccgg ggttcatcga taccgccatg acgcaggggt tgtcggcaga agtcaaagat
       61 gccctgcaga aacaaattcc attgggccgg ttaggaacgc cggccgacat cgccaacgcg
      121 gtgcgctttc tagtttccga ggaagcggcg tatattaccg ggcacgtctt gcacgtcaac
      181 ggaggcatgc tcatgtcgta acgatgtcga ttcagacgga tcaggatagg cccgaggccg
      241 agagccgacg gcgtgaacga gataacagga tggggaagga gggtggtaag gttatggcta
      301 cagtagatga acgggtcaag aaaatcatcg cagagcagct cggagtcgag gaggatgaag
      361 tgacgccgga ggcgagtttc gttgaagacc tcggagccga ctcgctcgat accgtggagc
      421 tcgtgatggc gctcgaagaa gagttcggga tcgagattcc cgacgaagat gccgaaaaga
      481 tcctgacggt cgggaaggcg ctggactaca tcaaggaaaa ggcatagccg tcgagggtgg
      541 taatgagtga tcgtacaacc agacgcatcg tcgtcaccgg cctcggggtc atcaccccgc
      601 tcgggaccgg agtggagaag acgtggaaag cgatctgtgc gggcgagtcc ggcatcggcc
      661 gcatcacgaa gtttgatccc acgggctacg atgctcagat tgccggtgaa gtcaaagatt
      721 tcgaccccgc ccggtttatc gagaaaaaag aaatcaagaa gatggaccct ttcatccact
      781 acgccgtggg tgcagcgcaa ttggcgactg atgatgcggg attgaaggtg gcgccggaag
      841 aggcgaccaa ggtcggcgtc tatattggtt cgggcatcgg cggcctggga tctatcgagc
      901 actatcataa tgtcctcaag gaaaaaggcc ccggtcgcgt ttctccgttc ttcattccca
      961 tgaccatcat caacctggcg tccggccagg tcgccatccg gttgggcgcc aaaggaccga
     1021 actcctgcgc ggtgacggct tgcgcgacgg gcaatcactg tatcggcgat gcctttcgac
     1081 tgatccagcg cggcgatgcc gacgtcatgg tagccggcgg tgccgaagcg gcgattacga
     1141 ccctcggggt ggcgggattc gccgcatcga aagcgctttc cttccggaat gacgaaccga
     1201 ccaaggcgag cagaccgttt gatcgggacc gcgatggctt cgtgctcggc gaaggtgcag
     1261 gtgtggtggt actggaagag ctcgagcatg cgcgccgccg cggtgtgcgt atctatgccg
     1321 aggtgatcgg atacgggatg aacagcgatg cctaccacat taccgctccg tccgaggaag
     1381 gcgaaggcgc ggtacgttgt atggaactgg cgctcaagga cgccgggatc ggtaaagatc
     1441 tggtcggcta tatcaatgcg catggcacct cgacgatggc ggacgttatc gagactaagg
     1501 ccatcaagca ggtttttggc gagcgggcct atcgtattcc ggtcagttcg accaaatcga
     1561 tgacaggcca tttgctcggg gcggcgggtg ggatcgaagc ggtgttcagt atcctggcgc
     1621 ttcaccacgg catactaccg ccaaccatca acttggataa tcccgatcca gcatgcgatc
     1681 tcgactacgt tccccataaa gctagaccgg cgtcgatcaa cgtcgcgttg tcgaattcgt
     1741 tcggattcgg cggagtcaat gcctgcctcc ttttcaagaa gcccgaggcc tgaccggccg
     1801 catcccgggt gcatatctct atgacatcgg cttcttccgt cgatgcgctc caatcctctc
     1861 tggcctatcg attccagaac ctcgctctgc ttgaagaagc gctcacgcac aagtcgcacg
     1921 tcaatgaaca gaaaacgccc gccggtctgc ataacgagcg actggaattt cttggcgatg
     1981 ccgtcctatc tttgctcgtg agcgagcgat tagtggcgct cttgcctcag tcgtcggaag
     2041 gcgccctatc caagctgaag gccaggcttg ttagtgaaac ggttcttgcg gaagtggcca
     2101 ggcggctgaa gctcggatcc tgtctgcgac tagggcgagg cgaggaactc tccaagggac
     2161 gggacaaaga ctcgttgctc gcggacgcgc tagaagcggt catcgccgct gtgtacctcg
     2221 acggcggatt ggacgcggga cgccaggtcg tcatccgtct gtttgcggaa gaatttgccg
     2281 aggtggccac gcaacagcac cggcccggat cagaggatta caagacgcaa ttccaggaat
     2341 ggtgccagaa acgtttcgac ggcctgcctc agtatgctgt tgtgcgagag tcaggaccgg
     2401 atcacgacaa gctctttgaa gtggagttgc gtttcaaagg tgatgtggtc ggcagaggag
     2461 tcgggcatag caagaaggaa gcggagcaac tggcggccag gcaggcgttg cagcaagccg
     2521 acgcgtgagg ctgacggtgg catggtatga tcacacagct gagaggccag caattgaagg
     2581 aggacagaat gcagcgaagg agaacggtta tcgtcatcgc ggtcagtgcg attctgagcg
     2641 gggcattgct gtggggggtc ggattaccgt ttgctgcgga taaggctccg gcaaaatctc
     2701 cgagagctgt catcaagacg aagtttggtg acatggaagt gacattcttc cccgagaaag
     2761 ctcccaagca cgttgagaat ttcatcacct tggcgagatc gggtttctac aatggaacga
     2821 tctttcaccg cgtgattcct ggcttcatga tccagggagg agatcccaac accaaggatc
     2881 ccaataagcc ggaaacgtac gggatgggag ggccgagcca caggctcaag gccgagttca
     2941 acgatattcc tcaccggcgc ggggttctct ccatggcccg cacgaatgat cccaacagcg
     3001 ccggctcgca attcttcatc gtggtgaagg attcgaattt tctcgatggg caatacacgg
     3061 tgttcggcga agtcgtcaaa ggaatggacg tcgccgatac aatcgtgaac cttccaagaa
     3121 atgcccgtga tctcccggct gaacgggctg aaatgaccgt ggtcgtgttg gagtagccta
     3181 tgggtcgagg ccatcagata gggaattctt ctatggccat gcgcgggttt gctctgtttc
     3241 tcgcctgtgc ggtcttgagc gcagtggcgg cctgctcggc aaggagcgag gtgaaaacga
     3301 cgctccctcc cccgtcggat cctcatccaa gagcaatcat caccacgaag ttcggagcca
     3361 tcgagatcaa gttattacct gagctggcgc ccaaccacgt cgagaacttt atcaagctgg
     3421 ccaagtccga tttctacaac gggacgattt ttcaccgtgt gatcccgggg ttcatgattc
     3481 aaggcgggga tccgaacacc aagaactcgc ttcgcaagga gacgtttgga atgggaggtc
     3541 ccaaggacga gaagggaaat ccgatcttgc tcaaggcgga atttaccgat acgcctcaca
     3601 agcgggggat tgtgtccatg gcgcggggga acgagccgga cacggcggga tcgcagttct
     3661 tcatcgtggt ggaggattcc catttcctgg acagaaagta cacggtcttc ggcgaggtga
     3721 cgaaaggtct cggcgtggcg gacaagatcg tcaaccttcc acgagacgaa cgtgacttgc
     3781 cgaacgaacg cgtggagatg aagattatta tcgtggaata ggaagagtca gactgaacga
     3841 ttacttaaag gtgtcgaggt atgccttgag acgcgcgcgt tcggatggga gaaagagaat
     3901 gaagctcatc ccgaagatat ctcccgtaac ccagcgcacg gccgcccgct cgatcttcac
     3961 cggtgacaat tctccgggaa gctgcaggag cacctgaaat tccagccctt cggccaggtt
     4021 gatctccgac tccgcccggc acccgccggt tgagatgttg gtgacgcggg catcgccttc
     4081 gatatcgccg gaggaaaatc ccaactgaca ttgcaagtcg acacgccgta acttccggat
     4141 cttcttttct ctctcaacgg ccataatctc aaacctgttc cggttcgcta gtgatgatgg
     4201 tgttcgcagc ccgggccatg tgtgtggggt tccggtgcgg ctggaggcat gaaggactgc
     4261 cccggcagta cgatatggcc cggctcatgc tgtttggtca aatgcgcggc gatctccgga
     4321 tgatacgtcc gcacatagcc gacgagcccg ctcaaaaacc gttgcgactg aaagtcggtc
     4381 cctgaaaacg ccaagataaa tttgatggcg cgatcgagga catcgggggc ggtagaggca
     4441 tcggcaatct gctgcgggtt ctgtttcttg aagctgtcgt attctttttt gaggtgctcc
     4501 gcaaacaccg gcatgggcgc tgacggcaca tggagagggc tgagggttcg ccgcaaagcc
     4561 tgaacggctg cgacgacttc cgcatcctgg ccgtctcgtc gcccatggaa atagctgaac
     4621 gtcacgacct cgatgagatt gtacagcgcc acggccttct ctccgcccag ttcgccaagc
     4681 tcctgataaa attcgcgccg catcggcatg aactgttcgc tcagacgttt ctgctggtag
     4741 tggcttcccg tcccgagata gacgcaatcc gacgggcagg agactcgcac aatccgatgt
     4801 tcgccgcagc attggctaca gatgagccct ccgagggccg gacaaggccg cttacctttg
     4861 cgttgggagc aatacgcgca ctgactcatt tgctcgcagc cccctcggtt gctggtttgg
     4921 gtggcggagg aatgaatcca tagtccgcca gggttcgttt ttcttgtttc ggctttccgt
     4981 cgtgcggagc ttccaagccg ttcatctctg ccgcatgcgc aaattcatag ggaacgagaa
     5041 tcaagttgtt cgtgcgatcg atactgatgt cgcccggcga aggtagatac tccgcaatga
     5101 cctggaagcg tttgtcccgg gtcatgcgcc agacttttcc cgtcgtgaag tccgagacat
     5161 acatattccc ccaacgatcg aagtcgacgc cgcgtaagtt ggcaaagcgg ctcgaaaaga
     5221 agccgttgga gaagagctcg gacatcgcgc cttcagggga aatttcaaag atcttccctt
     5281 tgtcccaact cacagcgatg agctggccgg atttcggatg gaccgcgagc ccggtcgggc
     5341 cggccaggtg cgggtcggtg atcagaatgg agactttctg tgtcgccagt tcgacccggt
     5401 agatgcggtt ggccttttgg tcggaacagt agagcaaacc cttcccgtcg gaggcgacgt
     5461 cggtcaggtg cgcgggggtc tgtgttgcgg cgccggcaat tggaatcgtc gccacggatt
     5521 tcccgctgta gatgtcgaac gcgcggatct ggtcaaggtc ggcgacgtac aaagtctggt
     5581 caacgagtgc catgcccttc ggggcatgga gcgtcacgtc agccttccct ccttggatga
     5641 atttgagatc gagcaatttc ccattggcgt cgagtttggt gataaagccg ttgttgtcgg
     5701 cggtttcagg ttcgccgttg acgctggaaa taaaatattc cttcccccat tgaccggtga
     5761 cgaaactata gggagattgt agttctgtca cagtaatctg ggcttcggcc aggggcgcga
     5821 gcgcatacag cacaaggccg gcacagcaag cgatagaatg gcggattgta tgggtcatgt
     5881 tgaaataggg tgcggcgagc ttattcaagg gtggagcaat gcctgttcaa gagtcatgat
     5941 tgtcatcgta gccgtggggg tgagaacctg tcaagaaaac tggtgtaggg taatggccga
     6001 gtaatggccg aaaggtagtg agagattgat gatcgtgagc ctattgcttc gggggcggcg
     6061 tttttgaggg ctctccaccc cttaccccta acgcctcacc gcttcgtccg gcgttgactt
     6121 gcataaaaat gccctgctaa ggtgcccacg gttttgaggc caacagcgtg atatcaggga
     6181 gggaatcgtg gcagctcgac tgattgacgg gaaagcgttg gcgcagcaag tacgggagag
     6241 gttggcgggg gagtcggcgg ccgtcctggc caagacagga atgaagccag ggctggccac
     6301 gatcttagtc ggagacgacc ctgcatctca tgtctacgtg aaaaacaagc aaaaggcttg
     6361 cgaattggcc ggtatctatg tcgacgacca taagttatca gccagcacca ctcaggccga
     6421 gcttctggcc ctgatcgaaa aaaagaacgc cgacccgaag attcacggta ttctggttca
     6481 gcttccgttg ccgaaacaca tcgacagcag agtgatcctc gaagccgtgt ccccgaacaa
     6541 ggacgcggac ggtttccatc catataattt cggtcggcta gtcgaaggtc atcccgtgtt
     6601 cgaggcctgt acgccgaagg gtgtgattaa gatgatcgaa tcggctggtg tcagcatcga
     6661 gggcaagcgg gcggtggtgc tgggccgcag caatatcgtc ggcaagccgc tggccctcat
     6721 gctcttgcag cgtaacgcga ccgtgacgat ctgccattca aaaacgaagg atttgccggc
     6781 ggtctgtcga gaggccgaac tcttgttggt cgcgattggg aaggccaagt tcgtcacggc
     6841 tgacatggtg cgagaaggcg cggtggtcat cgatgtcgga acgaacaagt tgccggacgg
     6901 caaattatgc ggagatgtcg atttcgaagc ggtcagtcag aaggccgggt ggatcagtcc
     6961 ggtgccgggg ggcgtcgggc ctatgacgat cgcaatgttg ctggacaata ccgtggagtc
     7021 tgctaagaga atggcaggga tgctgtaagg cagcagggtg ctcctgtgct cgcgcaaggc
     7081 gcggccttag aaagttctcg ttagacgcgc atacgaggga gtttcttagc cacttcttag
     7141 tgaaacaagg ttaggaaaga acacatgagc cgggagccta aacggttgcg agaggtactc
     7201 gatcaagggc agtttgccgt gacaatcgag tacaacccgc ccaaggggac gaacatcagc
     7261 ggtgtgctgg acagtgccag gcagctggtg gggcgtgtgc atggtgtgaa cgtgaccgac
     7321 aatacggcgg cggtggtacg cgccggttcc ttgcctgtct gccggttgct ctacgaattg
     7381 ggacacgatc cggtcatgca gatgacctgc cgcgatcgca atcgcatcgc aatgcagtcg
     7441 gatctcatgg gcgcccacat gctggggatt cggaacatcc tctgcctgac cggcgactat
     7501 ccaacggtgg gtgaccacaa agacgccaag ccggtgtacg atttggattc cgtccaggtc
     7561 atgcagcttg tacagggctt gaataatggt cgggacatgg ccgggaacaa actcgatgga
     7621 tctacggctt tcaatatcgg cgcggcggtg acgccggaag ccgatcccgt gggcccgatg
     7681 ctggcgaagt tcgaagtcaa ggtcaaagcc ggcgcgcagt ttttccagac gcaggcgatt
     7741 tacaacacgg agcagtttgc acgcttcatg aaagccgtcc gtccgttcaa ggtcaaagtc
     7801 ttggcaggga ttctgttgct gcgtaatgcc aagatggcgg agttcatgaa cgccaatatc
     7861 cctggcatgt cggttccgca ggaaatgatc gacgaactgc gcgcggccgg cgacaaatcg
     7921 gaggacgttg gtgtcgagat cgctgtccgc accattaatg cggttcggcc tcattgcgat
     7981 ggagtccaca tcatggcgat caaaggaatc catcgactcg gggaaatcat aacaaaggcc
     8041 caactcggat catgacggtt cctgacctcc tacgatacaa acgcgaaaag aaaaaagtga
     8101 tcgtcgtgac ggcctacgac gcgctgttca cccgcatcgt tgagcaggcg ggaatcgaag
     8161 cgattctcgt cggcgattcg ctcggcgtgg tggtccaagg gaaggccaat acgctctccg
     8221 tcacgatgga ggatatggtt tatcacacca gacttgtcgc cggtgccgct caacgggcga
     8281 tggtcatcgg cgatatgccg ttcatgtcct accaagcgag caaagaggat gcgctccgca
     8341 atgcggggcg gttcctgcag gccggagcgc atgccgtcaa gctggagggg ggcgcggtcg
     8401 tgctcgatcg cgtggaagcc atgactcaac tcggcatccc tgtgatcggt catctcggca
     8461 tgacgccgca gtcggttcac cagtatgggg gatataaagt ccagggcaag gggaaggacc
     8521 aggctgaaac gcttctgtca gatgcaaagg cgctggaggc ggccggggcg gtggcggtcg
     8581 tactggaagc catcccggct gaactggcca aggcgatttc ggaacaggtg gcaattccca
     8641 cgatcggaat cggcgccggt tccgattgcg acggacaggt tctcgtgctc tacgatttgc
     8701 tcggcatgtt cgacgagttc gttcccaagt ttgtgaaacc ctatgcccat ctcaaggccg
     8761 atgccctgca ggcgcttcgg cgatataaag aagaagtcga acagggaaag tttccttccg
     8821 actccgagtc ctatcactga tccttcgttc ggatcgcttc tatttcccga ttgacatagc
     8881 taagcgaatg ggcggcgcct gctcatacga gcgcatgatg cattgacgca ggtcattggt
     8941 gaagccggcg acgtcgagtc ccatatagaa ctccggtacc ctctgcagtc gaacaaggcc
     9001 gctgtgcagc agattttgcg ccgcttggtg attgcccatg aacagtttca gattagctgc
     9061 ggcgagttga atgaggcctt gaaagaaatt gccctggtcg gattgacgtc ccgcgacatg
     9121 ccagagagcc tcaaagactt catggcattc ccaccagtag gcgtaattgt agagatcgac
     9181 cccgtaacag taatcatctg attcccgcca ttgttcggga gcgaagggcg tcggatctgg
     9241 ttctccgaat ccctgtgaat gcccctcggg cttgcgtcgc gggtgctggt tgttccccgg
     9301 cacaaaccgg tatgagggga aagtgcgtgc agagtagcga ggccacgagg gatcaggtgg
     9361 ttgcggttca gtcacggcga ggcttatcgg cataatcaac ctgaaatacg gaacggtctt
     9421 ccagccggat ggccgtcaac tgtctgcccc atacacatcc gctatcgatg gccagaagtt
     9481 cgggctccat gtgcagcccc aatgccgccc agtggcccga aatgatggtc gtgccgcggt
     9541 tgcgccgatc gagaatgcga aaccatggag tgaagccggc gggagcttca tccggcggcc
     9601 ccgagaagtt cgacatctca ccggtcgaag tacaggtgcg aagtctcgtc agcacacgag
     9661 caatgctgac gagacgttcc gatcccttga gcgaggagtt ccagtgcggc gttggcccat
     9721 ggaagagcgc cttgagaaag gtgcgatagc ccgggccgtg cagggcggtt tctacttctt
     9781 gtgccagggc cgttgcctct tcaacggtcc attgcggcag gagaccggcg tggaccacga
     9841 gaaaagatcc ttcacgatga tgaaacggtt ggcgtctcaa ccactcgagg agatcggatc
     9901 gatcctcggc ggagagtatg tcgcggatcg tgtctttggg ccgaagggct gcgatgcctt
     9961 cagccactgc aagtagaaag agatcatggt ttcccaggac aagccggacg gaagcgccaa
    10021 gattcttgat gaaccggagg gtggcgaggg aatcagggcc gcggttgacc aggtctccag
    10081 ccagccagag cttgtcggtt ttcggagcga aagaaatccg ttcgagaagc tgttggagcg
    10141 aaacaaagca gccttggatg tcgccgattg cgtaggtggc catcagtgaa tgcgctcggg
    10201 cacgagtgga tcggggcgtc gtggtctgct aggggtagcg ggcttcgatc acgcttcgca
    10261 ggccgcctct ggcggcaaat gtgcccgtga cgttcgccca gaccggatgg cacgccttcg
    10321 caatatcctg aaggatgcgg ttgacggagt tctcgtaaaa aattccgatg ttccggtacg
    10381 cgtgaatgta catcttgagc gacttgagct cgagacattc cttgtccggc atatagtgga
    10441 cggtgatcgt gccgaaatca ggcaagccgg ttttgggaca gatggcggta tactccggaa
    10501 tctcgatcgt gatctcatac cccttgtatt gattcggaaa cgtttcaata tccggcaacg
    10561 gggaggcaat gccgcttttt gcgtgccgct cgttataccc cagcttcgtg gtccgctggc
    10621 gggttgaccc cttacccctc acgcttcacc ccttacggtt ttgactagac ctgcttcatc
    10681 cactcagtgt gcccgatctt ttccaattcg ataaagagcg aaacccacgg gcatttcatc
    10741 tccgggtaga cctcacagag gccccccttt cgaacccctc cacaggggcc gtggctcatg
    10801 aactttgggc attcggcttt gagggagtcg tcggggatcg gcggccgctt gtgtacgcct
    10861 cccacatgct gggctccttc gtcctcgccc gcgatcagta cgcgtagttc catagccttc
    10921 tagtctagac agatttcaca gcgggggcaa tgtccctttg ttattttggc gattcaccca
    10981 gtggaattgt cttctgcatg ctttgaatgg ccgtgcttgg ttagggcgaa tggcctatgg
    11041 ggatggagtg agctaggcct tctttcggtg gcggtgggcc ttctgagaag ttgctttgat
    11101 tatcgcagcg ttatacactt ttaatcgatc cggagagatc aacgtccggg caacattgtc
    11161 gttgacagtc ttttttgggc tttgctacga tggccgcacg tttgaagagt tttgaactga
    11221 aacagaccgt tcttccagcc tcgaattcct gcgggtttgt tgcgtacaat tgttctattg
    11281 gtcaatgcgg caacgggtgt ccgttgcaac gagagaggga ggtgtcccaa tgagtctcga
    11341 ttacgtcaag ttctccaatg gattcgaaaa gttcatgccg aaggaatatc gggatatggt
    11401 cgagcatggc cccttcggga agaaaatttc cgtctcgcag atgggcagct ttaaagaaat
    11461 tcttgaggag catcccatgt gtgccggctg cgccatgacg ctcttcatcc gtttggcgat
    11521 gatcgcgttc cccaatccgg aagataccat cacggtcggc acggcaggct gcggccggtt
    11581 ggccatttcc caggcggcga ttcctttcgt atacggcaac tacggcgacc agaacggcgt
    11641 ggcgagcggg ttgacccgtg ggcttcggct ccgcttcggc gacaagccga aggatgtggt
    11701 cgtcatggcc ggcgacggcg ggaccgccga catcggtttc cagcaggttc tccattcctg
    11761 gtttcggaaa gagcgcttca ccacgatcat gctggacaac gaagtctacg ggaataccgg
    11821 cggccaagag agcggcatga cgaaccgtgg agccgtgttg aagatggcgc cgctcggcaa
    11881 gaagttcgaa aagatggata tggtgcagat ggcgaaggtc gccggctgcg cctatgtggc
    11941 gaccgtcgtg ccgaacaatc cacgccgtgt cgagagcgtt attaagaaag ccgtgttgat
    12001 cgcccgcgaa gtcggttcca cctatatcca ggcctatacc tcctgcaaca tcgaatacgc
    12061 gattccgacc gacaaggtca tggaagacgc gaagacggtc gagaacgatc ggtatgcatt
    12121 ctccgaatac atcagcgacg aagccaaaga gtatttgacc gagcggtatg gctataagga
    12181 atttctcccg aagcctgctg ctcctgccgc ggcaatcccg ggcaaggcct aagctgataa
    12241 aaaggaggct ggaccatggc aaagcgtttt aacattcgga tggcaggcgt cggcggccag
    12301 ggtgtggtca cgggatcgca cattctgagc acggcggtca tcaatgccgg cggcgaaagc
    12361 acgattgtgc cgttttacgg atcggaaaag cggatggcgc cggtcgaaag ctacgtccgc
    12421 gtgtcggatg aacctatcta cgagatcggc gagattacat tcccgcacat catcatcatc
    12481 ttccacccgc aggtgatcac gcacggcaag tcctatacga tgccgttcta cttcgggttg
    12541 aaggaagacg ggatcgcctt gatcaacaat gatgggccga tgaacctcca caaggatcag
    12601 gcccgtgagt tggaagaacg tcgggcgaag ctctactatt ttccggcgac caagatctcg
    12661 ttggaagtcg cgggaatgga tctcgcgacg aacatggcat tgatgggctg tatcggagcc
    12721 atcacgggtc tgacgaacat gctggggttg gagcaggccg tgaaggaccg gttccttggg
    12781 aagggcttcg tggtgtccgg cggtacggcc gcgctagaca gcgtcgttga acggaagttt
    12841 aagaagaagg cagaattgat cgagaaaaac gtcgccgtga tgcgggctgg gtggaactac
    12901 gccgtcgacc atggttgggc agcaccagaa gtgaagcgtg cggaagagcc tgtggccacg
    12961 gccactgcca ccgcgtaaag gagtacccat gtacctcgta gccgatatca acgttgaaat
    13021 ctgtgcatcc aagagctgca agctctgcac gcagtattgt ccggaagcca ataccattct
    13081 ctatagtgaa gagatgggga gggagaaagg gttcaagtac ggatctgcct atgtggcggt
    13141 tgatcggtgc aaaggctgtg cgcagtgtgt ctgggtttgt gacaatatgg caaaaaacaa
    13201 cgccatcaag atgattatga tcgatcaact gccgaaggcg gcgttgacgg acaacatcac
    13261 ctacggcgag aagagcacga cggcagtact ggcaagccca gtcgtcggat aagcatcgga
    13321 aaggggaatg ggcgtgggaa caacgcaaga tacgagagag cggattatcg ttccagggcc
    13381 tgcagggttt catcctccgt cagcggcaca gcttggcgtg gccttgcctg atccgggaga
    13441 aggacttttc tacgggctgc tcgagcccaa cgaagagaaa gtcatcgaag agatggcgcg
    13501 aaagatgctc accagcccga acgccaccat cttcccagga ccattgctat tgtgggcctg
    13561 gaacgagcat gcggtggaaa aggcgaaggc gacgctggaa atcgcggcgc agatcccaga
    13621 ggtcatgatt atcccgatgc cggactaccg gccgaagtat ccgaagatcg atccggaaga
    13681 agtgatcaat cccaaccatc cgaacctgac gatttggggt aacaagatcg aagcctgcat
    13741 tttcatcggc gtccattgcc attacgcgaa tctgaccctg aagatgattc gggctgggac
    13801 aaactgctgt accatggcgg tttgcgccga acaggggcac gaagatgcga tgctgacgat
    13861 ccgtgattcc gacaccgcaa agattaagcg ggtggcgcag atcttcaaga aggtgcgtga
    13921 agaaatgggt atcaagttgc cggagaacgg cgagaacgtg cgtttcaccg gcacgcagtc
    13981 gaaagtgcat ggtggaaaga cccataccaa tccgatggcc tttgcaccgt ctcctgccgg
    14041 cgccggcagc gctgcggcgt tcggtcattc tcctgaacaa atgaaacgcg aaggataggc
    14101 tgagcaacgc agctcaccat tttttgagag gtcgatcatg agtgaagcct tagaagccca
    14161 acagaaaaca aatccgcaag gcgatccgat caccagtgtc gccgctccga aattggatgg
    14221 gaaaaaagat ccccatgccg atgccaagaa gcagagaata gtgacgcccg agtatatgtt
    14281 ccaagaagct ccgcggacca aagagtttat taccgggagt gaagccgcga aggaagccat
    14341 tcggcgttcg aacgtcgact tggccattgc ctatccgatt acgccgcaat ccgaaaccat
    14401 gcagctggtc ggcgtgctct acggcgaagg ctatgtgaaa gagtattacc gcggagagga
    14461 agaagtcggt gtgatggcgg cgatcgccgg aggatcccgg tccggtgtcc gttgttatac
    14521 ggcgaccgcc ggccctggta ccttgcgagg cctcgaaggc atcgcgtcat ggcccggaca
    14581 tcggctgcct gtggtagcca tgtttacctg ccgcgtcgtg aacgcgccgt tggccattca
    14641 accggacaac attgaagtcg cctacctgct gaactgcggc atgatcgtgt tccatgcgga
    14701 aaaccagcag gacatgttcg atttcacgat ggcggggttt gtcatcagtg agaagaatga
    14761 cgtcactctg cctgtcggtg tctgttgcga cggatttttt gtgacgcacg cgcgcggcta
    14821 cgtccgcatg caggaacgcg gcattaagct tccgccccgc gaggcctggc gcggtgccgt
    14881 gccggtgctc gacgcggaga atcctcccgc tcggttatcc cgcgacgccc cggtccaaaa
    14941 atcgaacttc atggcgtaca acatccatgc ggtatggcaa caggaagtct gggctgcggt
    15001 cgaacgctcg cgtaaataca tcaatcggta tatgggcgga ttgctcacag ctgagaacgt
    15061 tgaggatgcg gaggtgctca ttattgcctc cgggagcgcc gcagcccagt cgcgcgaggc
    15121 ggttcgcctc tgcaaggaaa aaggcatgaa ggtcggcttg atcaagattc gctctttgcg
    15181 cccgttcccg accaaggaac tgcgtcaatt gtgcggaaaa gccaaactga ttgtgatccc
    15241 ggagttcaac tatgtcggat ggctggccaa ggaagtggcg accgcaatct atggtttctc
    15301 taaggcgaag gtcatcggtg gtccgcgtgt gtacggcggt cagtcgatgc cggtggagtt
    15361 gatcgtggac gaagtcgagt ccggtgtgag cggcaagaag tcgacgaatg tcgcgatgtc
    15421 ctcgatcatg ggcggtgccg tcaatcctga cgaagtcgca catttcatgc gcagcatcta
    15481 gtaaacgcgt tgtgatagaa acagaaaggg cccgtccgtt aaaccggacg ggcccttcgt
    15541 ctttttcagt ttgtttcgtt catctagatt gtttggttga acaagacaaa ccagatagac
    15601 cagacaaacc attcaaccct ggttcctcta cccttgaatt tcgtcctcga ccatttcttc
    15661 gctcgccggc atgccgtaag ccacgtattt ggcataggac aggtagatca acgcactatc
    15721 cctcatggcc tttgagccat gcgtcatcct gaccaccttg tcggatccag gcggctccat
    15781 gttttgtaac gtggtcacat gatcctgcag cagtgtcgtg tatcgttcga tggccagctc
    15841 gacttctttg tacgcttgta gaatggcgtc tgtcatggtt gtcctcctcc gctcgatcat
    15901 catacactag ggcatggagc ccttcaaagg caagccaccg gtgcgaatgc cccccctcct
    15961 gctcagagcg cttgagcggc tgaacgacgt cgaatcccta ccggggtcct ccggttatgc
    16021 agaggcagtg gcggaacttt cgcggctctt cacgaaggag agggtagcgt tgcgaagcgg
    16081 atatcttgac gatcccgatc atgctgctgc ctatctgcgc tacttcctgc cggtgaatct
    16141 gtccaagatc caggtgctcc tcgatgaaat gccggaagca acgctgatcg ataagcaggg
    16201 gagtgggctc aacgtgctgg atctggggtc tggaccaggg acgggagcct tagctgtcct
    16261 tgattggctc catcagcgcc accctgagca ggccgggaag ctcacggttg tcgcgatcga
    16321 ctcgtccgct gatgcgctaa gtcaggcagg acaactctgg gacagctact gccgggaatc
    16381 cgggattgcc ggcgcgcgcc tcactcctta cgaaacggac cttgagcgtc ctctgaatgg
    16441 accatcgggc ggtcagatct ataagaacgg tccatacgat gtcatcatca tggccaactg
    16501 cctcaatgaa ttattctccc gcgcagtcga tcccatcggt gcaaggaccg gtctgctggc
    16561 cggacttctg acgctgctcg cgccacatgg aaccatgatg ctggtagaac cggcattacg
    16621 gaccacctcg agagagctgc atgaggttcg tgaccggtta ctccatgaca gacgttgcac
    16681 ggtctacagc ccttgcctcc atgagcggcc ttgccctgcg ctggtgaatc cagacgactg
    16741 gtgccacgaa gagagagctt gggatcctcc agctgccatc caagcgattg atcaggaagt
    16801 ggggttcatc aaagatgcgc tgaagttttc ctatctgttg ctgcggactg acggacggat
    16861 gattgtggag cgacggcctg acgtgtatcg ggtggtgagt gaactgcgcg aactgaaggg
    16921 ggagaagcgg gcctggctgt gcaacgaact cggccgccaa gaagtcggac ggcaggatcg
    16981 gctggcttca cctgagaatg ccgctgtcga tgcgtggcat cgcggggcga tcgtgcagat
    17041 cgagcggatt gtgcgaaagg agatgggtgg caaggtgtcg gcgctgggac gaatcgagca
    17101 agacgcaaca gtcaagattg tcagacccgt atgaatggtt tgaacagggc tggcagggct
    17161 aatcccttgt gctcgtgcaa agcggcacgg cacatgggtt ttatcccgtc gccaggatca
    17221 gtaataatgg ctccgggccg aacaagttgt gtttggcgaa gctcgttgga cgcgcgcacg
    17281 ttagggatca gccctgccgt ccctgggtaa aaaatggggc ggggacagac cgctaatggg
    17341 cctgtccccg ctc
//
LOCUS       DATIFR010000048        25536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2711652, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000048 DATIFR010000000
VERSION     DATIFR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711652"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VLD60_02485"
     CDS             <1..944
                     /locus_tag="VLD60_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSB43846.1"
                     /translation="GGALGKTDIELFPRAQAEQFQTHDRQVLESGVAKEFEEVAWQAD
                     GFHTSIVVKFPLRNASGQIHAIGGVTTDITDRKRTEEALRESHQALRRSQEDLRELAG
                     CLLSAQDDERRRLARDLHDDMNQRLAALALDLEALARSLPPSLIEARHQLASVQHQVV
                     TISDDVHDLAYQLHPSLLDDVGLEVALQDLVRSFGQRENISVLYNPQNLPDSIPTPIA
                     GCLYRVTQESLRNAAKHGKASQVVVDLFGSPEGLDLSVKDNGKGFTAEEGKRPPHRGL
                     GLISMQERLRLVNGVFTVESRPGQGTKICAWVPVREE"
     gene            941..1594
                     /locus_tag="VLD60_02490"
     CDS             941..1594
                     /locus_tag="VLD60_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB43847.1"
                     /translation="MIVARPRILMADDHSIVLAGLRKLVEEEGDVVGTVEDGRALVEE
                     AQKLCPDIILLDISMPLLNGLDAARQLTKLVPESKIVFLTMHATPTYATEAFKAGASG
                     YLIKRSAATELKDAIHAVMKGQHYLTPLITKDVLDATLHPPRGHARRRPPASLTARQR
                     EVLQLIAEGKGMKDIASVLNISVKTVEFHKFRIMEELDLHSTAALTKYAIAEGLVSL"
     gene            1717..1983
                     /locus_tag="VLD60_02495"
     CDS             1717..1983
                     /locus_tag="VLD60_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43848.1"
                     /translation="MAVEGMIQEVHDALDKFGAACPMEEAVALCPNLTWNQVFLAIDY
                     LSRTGQVRVLQDPARTYRVQVLHPTGSAKSFPATAPLAGVGSGK"
     gene            complement(2590..3213)
                     /locus_tag="VLD60_02500"
     CDS             complement(2590..3213)
                     /locus_tag="VLD60_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43849.1"
                     /translation="MKELRRLLICGVSLVALAGCAGTGEVIPLQIHPLNTGSGSVSKP
                     AAPLRVAVGPIEDGRAYKTGLGVRTHLWGGVSYFDVPGGNPMEVVAQTLSDYLTARGW
                     QVTKRGSSDSADVILTGKILDLSVHAKSRVGFTEISTKTRLVVQARNVADESMVRMTL
                     NGSGSDDEFWFDTEDIEELVNDVLTDSFGKLVHDTTVDNRLLRLKTP"
     gene            complement(3284..3946)
                     /gene="pal"
                     /locus_tag="VLD60_02505"
     CDS             complement(3284..3946)
                     /gene="pal"
                     /locus_tag="VLD60_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSB43850.1"
                     /translation="MSMRYRLSYFTFPLCLLVVVMLSGCASRKVVTAVEDQSFTPGAA
                     PKAQAVEEAKVAPPAPAKPEIPAAATVEPPKAEVPPAKTPAAEDMRIVEQPVAPAPAP
                     RPAIEPAPPVAELSDVFFDFDRFTIRGDARAPLEANAGVLKMQPGTKILIEGHCDERG
                     TSAYNLVLGERRAQAARQYLQDLGVPSSQLQITSYGKERPFCTDHSEACWQSNRRAHF
                     TR"
     gene            complement(3960..4298)
                     /locus_tag="VLD60_02510"
     CDS             complement(3960..4298)
                     /locus_tag="VLD60_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43851.1"
                     /translation="MKVIMSYLGLIIVSGFLTLTTPMTNATAETIQRMVRGTVTATNS
                     TVDPQTIVIKVMLPNKEELTVGARVPTDTKITRGTKTVRLADLKVGESADITYLKSPD
                     GLVARSIHVR"
     gene            4741..5271
                     /locus_tag="VLD60_02515"
     CDS             4741..5271
                     /locus_tag="VLD60_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HSB43852.1"
                     /translation="MSTRRPIFNVQKHQASHLHYDFRLEIGGVLKSWAIPKGPSLDPQ
                     VKRLAIEVEDHDPAYADFEGIIEEGHYGAGPVILWDRGWFEAREEGISPEAMWRAGTL
                     EFVLHGRRLRGGFALIRMKGRPSQWLLIKRRDKEARPDSEMPEEWNRSVVSGRTIADL
                     NRAAEAGELEAYRCGA"
     gene            5259..5855
                     /locus_tag="VLD60_02520"
     CDS             5259..5855
                     /locus_tag="VLD60_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSB43853.1"
                     /translation="MRGLMTRDQTLHDNGSEHRWTTPWNVLATARNHEHGRLARRLKR
                     FGDFRWAPYLGVLIGRVEDHEAFFEQLRRAGEVKPGFLFPLARLVPLDRTFVFATETL
                     IPLLKAELLTAAEQIGSGSFYIRIERRGHKGEIHSREIEQELAHALIASLEERGRTPH
                     VDFHDPDVIIAIEIIGDECGVGFITKSLRERYPFIKVP"
     gene            5888..6124
                     /locus_tag="VLD60_02525"
     CDS             5888..6124
                     /locus_tag="VLD60_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43854.1"
                     /translation="MTVSRHVEEAAGKLKEASRQIALAREGPATCENQKIWLEALTAY
                     CEALADIHTYNNESIHEKLHELAGRTGLRKFPAS"
     gene            6239..6799
                     /locus_tag="VLD60_02530"
     CDS             6239..6799
                     /locus_tag="VLD60_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HSB43855.1"
                     /translation="MDVMEAIYQRRAVRAYTDQPVDKATVEVLIKAATHAPSSMNEQP
                     WAFVVLQDPKRLASWSDRIKAHVLLRLKPDSPLAKYRDMIADPDYHVFHRAGTLIIIC
                     ARQGAHNGEEDCSLAAQNLMLAACSMGLGTCPIGFARPWLNQARIKRSLGIPDGYVPV
                     FPVVLGYPIGETKPVERRAPEILFWE"
     gene            6809..7726
                     /locus_tag="VLD60_02535"
     CDS             6809..7726
                     /locus_tag="VLD60_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HSB43856.1"
                     /translation="MTSGKPLQVAIVGFGRVGQVCAEFISMSHDLSLAAIVRRIATAG
                     GKLPEQLRRIPVITRIGQVKDVRAALICVPTNVVEETVSQILPHGIPIVECATLHGDA
                     LHAHKEMIHKLALRYQVPAIIGAGWDPGALSIFRSWFALLTPGGETETRHHTGISLRH
                     ATMARSVTGVKDALCAEVRTAEGRFQRYVYVELEKGADPDKITQAIRADPLFLGADTQ
                     VFSVESLAALEQEGRGVVIDRRGPPRRLGHQHLLLEARFEESVLTAQVMLAAARAVPQ
                     LEPGAYFLSEIPLSAMWGEMMEKAHRDWL"
     gene            7801..8292
                     /locus_tag="VLD60_02540"
     CDS             7801..8292
                     /locus_tag="VLD60_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSB43857.1"
                     /translation="MMRLYAVTKPEEQQLAWEAFPSWAHFTWLYLLSAVSALRGGLFF
                     KFGVDGWEMWVIGAGLLLVCAAILRHWAHYEISQGQITVRNGYTRHEIQSIPLSDVGD
                     VEVQQGRIADFFGIGTIVVHPRSNDRLLSLRGVKDPEEIMIRIKALAWKHNRTGSQPR
                     VSS"
     gene            8391..9380
                     /locus_tag="VLD60_02545"
     CDS             8391..9380
                     /locus_tag="VLD60_02545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HSB43858.1"
                     /translation="MKIALTGDVMLGRLVDQYVIQNRSVRPEALWGDVLPIMLAADCR
                     LINLECVISDRGEPWHPTTKAFHFRARPRALEFLQAAKIDGVTLANNHVLDFGADALL
                     DCLNLLDRTGFKRTGAGAKLEEALVPVLLRLPEGRIAVVALTDNEPEWEATDTKPGVN
                     YVTYNDRGLNEPYRSRVAQVLLSARRQADLVIVSAHVGPNWGAPSRAMQALARDLLDM
                     GADLYWGHSNHTPQGIECYNGKAILYSAGDFVDDYAVDKDERNDLSFLFILEAERGRI
                     ARIRLHPTCIEDLGVRVANEQERQFLLRTMQTKCKVFGTTMDVERQVGTISVK"
     gene            9469..9741
                     /locus_tag="VLD60_02550"
     CDS             9469..9741
                     /locus_tag="VLD60_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSB43859.1"
                     /translation="MGVRGICPTNEAAFEGAGLALTAMITDLGAVAPTQSVPIACEAP
                     DDELLLVDWLNALVYEMAPRKMLFGRYGMAKTPQEGSRKVPNGKWL"
     gene            9759..10106
                     /locus_tag="VLD60_02555"
     CDS             9759..10106
                     /locus_tag="VLD60_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB43860.1"
                     /translation="MLPLQELRVKLLAQRRDLFRQAAKTEEDLLWLETDVEREAEERG
                     QTETMVRLLDRLDEREKREIEAIDLALTRIEAGSYGRCERCGKEIPVARLQALPTATT
                     CLACGEAGEALKR"
     gene            10103..10354
                     /locus_tag="VLD60_02560"
     CDS             10103..10354
                     /locus_tag="VLD60_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43861.1"
                     /translation="MKLGMGPDWRVTVHVECSAGHRGEEVPRAFILGDRRIDVVAVLD
                     CWPGRDHRYFKVRGGDSGLYILRHDTPPDEWEITLFEQR"
     gene            10571..11680
                     /gene="dinB"
                     /locus_tag="VLD60_02565"
     CDS             10571..11680
                     /gene="dinB"
                     /locus_tag="VLD60_02565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSB43862.1"
                     /translation="MRIVGHLDMDAFFAAIEERDNPLLCGLPIVVGADPRGGKGRGVV
                     STANYKAREYGIHSALPISIAWRLSEEARKHGQPPATFLPVRMQRYAEVSAHVMESLQ
                     RFVLLVEQASIDEAYFDLSSCESYTLAEEVCRKVKRAIRSEEYLTASVGIGPNKLVAK
                     IASGMSKPDGLTTVREEDVAEFLAPLPVRTIPGVGPKTESELAAVGVELIKDMRRFSQ
                     DELRAMFGKRGMDLYEKIRGRDESPIEETHEVKSIGEQETFEQDTGNSPFLVERLRAL
                     CRDVMRRLTTEGFTHFHRVVLTVRFADFETKSRSHTLPVPMNSLNVLQGEAMNLLLPF
                     LDDRENPQGKLIRLIGVRVEKLEQFGVHSQTPLIE"
     gene            complement(11748..13130)
                     /locus_tag="VLD60_02570"
     CDS             complement(11748..13130)
                     /locus_tag="VLD60_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB43863.1"
                     /translation="MQIERGEQDPSTLPRHIGWFTAGCVLVSNVVGSGIFTTTGFMAR
                     DLGHPGLILSIWLGGALIALAGALSYSELGAAFPVAGGEYAYLRRAYGPFVGFLSGWT
                     SFTIGFSAAIAAGAVSFAAYLFQILPLDDEGGIRSTAIALALLWFITGFHLAGVGAGG
                     WLQRTLTVLKVGAILLLIAAGLMVGRGDWAHLTPTDTPPLPGIGPYVVSLIFALYAYS
                     GWNAAAYLAGEITDPARTIPRTMIGGTLFVALLYLLLNGFYFYALPVTELAEPPILPV
                     ADKVARALLGIEAGRFVTVMLCLSIAGAVSAMVWAGPRVYYAMAQDGLIPSLFASTPG
                     MERTPINAILLQSLWASALILSGTFERLVIYSGTVLMIFSALAVGAVPILRRREPDLP
                     RPYRTPLYPFVPGFYLLVSAVIVGVALYERPLEGGLGIATVLAGTPLYLLWQWHRRAR
                     QPQDSSGQQH"
     gene            complement(13134..13895)
                     /locus_tag="VLD60_02575"
     CDS             complement(13134..13895)
                     /locus_tag="VLD60_02575"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSB43864.1"
                     /translation="MKSLIRSSTPLVPLCFALIQVLMGASSCNGDRAADSPLIDPNRK
                     TERHRMVDEHIIPRGITDQAVLAAMRHVPRHRFVPAMYAAFAYIDGPLPIGHGQSISQ
                     PSLVAMMTEVVTLKKTDKVLEIGTGSGYQAAVLAELADKVLTIEILEPLARQAERTLT
                     ELGYNNVRVRVGDGYQGWPEEAPFDAIIVTAAPDHVPQPLLNQLAVGGRLILPVGRFY
                     QTLELYRRTTEGYEHKTLTLVRFVPLTRLGEEKEE"
     gene            14207..14917
                     /gene="pcm"
                     /locus_tag="VLD60_02580"
     CDS             14207..14917
                     /gene="pcm"
                     /locus_tag="VLD60_02580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HSB43865.1"
                     /translation="MTQGWIIPPQTEPRKSRDELQKEREDKIRQLIADGLLRSERIRS
                     ALLTVRREDFIPPRYRDYAYQEVPLPLPGTRATISCPHSYPLFYEPLGLDVGHRFLEI
                     GLGSGYGAAVARQVVGPTGVVVSIEIDPVTYEFGRTNLERAGYHDVRVVLGDGGQGSP
                     EHAPYDRICITAACGAVPPPLLEQLTVGGKLIAPIEEQGIQNLTLLTKGEQGLNRQVI
                     CEVLYIPLRGRYGQVPSG"
     gene            14957..16165
                     /locus_tag="VLD60_02585"
     CDS             14957..16165
                     /locus_tag="VLD60_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSB43866.1"
                     /translation="MIEPMIIESSWMSPAREAVEIVERKGLGHPDTICDAVMESVAVE
                     LAQAYLKTCGRVLHFNADKAMLVAGQVACRFGGGSVLAPMRLVMGDRATFEWKNKHIP
                     VKEIAERAAYKWFKRHLPRIKPLKDLECQVELRPASEELRSVVERPREAVANDTSATV
                     GYAPLTPTERLVFQVEQFLNGPSFKKEFPDTGQDVKVLGVRTGRDVNITVAMPFLAPL
                     IRTESEYFKRKASARQALTAFVKRKGVAGFSPQVFLNELDQSGAGEAGAYLTLLGTSA
                     EAGDSGEVGRGNRVCGIISLRRPASAEAAAGKNPVAHVGKIYNVLAQVLAEDIHKKVQ
                     GLQDVTVWITSQIGKPISSPQVVVVEVTLANGTTLAVVRPQIQREVRLAFTHIKSFCK
                     ALTRGVYAVC"
     gene            complement(16190..17005)
                     /locus_tag="VLD60_02590"
     CDS             complement(16190..17005)
                     /locus_tag="VLD60_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HSB43867.1"
                     /translation="MRDAEGVQFLQWCLPRLRLIWPGYRKVRRQVYKRIQRRLQLLDL
                     PSLADYQVYLETHPEEWTVLDSFCWMPISRFYRDKSVFQFLEQEVLPSLARQALAQGE
                     SVLRCWSLGCAAGEEPYSLSLLWKLKLQAQFPTVRLIILATDIDDQAIARAQQGCYPL
                     SSLRDLPESLRAQAFDHTAEGFRIKPEFREPVTFLVQDIRKAAPEETFDLILCRYLAF
                     TYFDATLQDTTLRQLTERIRPGGAIVIGNGESLPEGEFGLIPWSENEGVYRRV"
     gene            complement(17056..17664)
                     /locus_tag="VLD60_02595"
     CDS             complement(17056..17664)
                     /locus_tag="VLD60_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HSB43868.1"
                     /translation="MQAGSERHTADISGSGHTSTIIGRAPHILITGTPGVGKTTLTRE
                     LARQLADHHPAGFYTEEIRDAQGTREGFRLVTLCGHQLVLSHIHHPGPYRVSRYGVDV
                     HGFEQLLSDLDLSHTQSRLVIIDEIGKMECFSRRFTDDITALLNQPKFLVATIALKGE
                     GFIRHVKDRPDCRLITVTRENRDRLLSELAAELKNSLPEGTR"
     gene            complement(17676..18029)
                     /locus_tag="VLD60_02600"
     CDS             complement(17676..18029)
                     /locus_tag="VLD60_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB43869.1"
                     /translation="MITSIAFTVYPVSNMERARAFYEHVLGLHVSWHYQDIWMEYDLG
                     ASTFAISTAETGHTAGAKGAVVAFEVSDFDAFVQKMKERAVAFVTEAFDTPVCRMAVI
                     EDPDGNHITIHKRKS"
     gene            complement(18105..22358)
                     /locus_tag="VLD60_02605"
     CDS             complement(18105..22358)
                     /locus_tag="VLD60_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HSB43870.1"
                     /translation="MSYVGVNIGALTVKVAALRGDARSATVLAHQGRPLEILDEVLAR
                     AEFADAEYFGVSGQLGHISEVAAVQRALREVDGTFDAVASLGGESFLVYLLMEGRITN
                     VVSHNKCAAGSGEFFVQQIGRMGLGMEEAIQRSFSGKVVPLASRCSVHCKSDITHKLN
                     RNEATPEDILHTLHDSMSNKVIALLEKGTRNLNRVLLIGGVTRNEAMLASLRAKLPAT
                     EFVVLPESSWFEAWGTALLVRDSPCDTSPKIAAQPGLGRLPPLHLYGERVQVIAAPPH
                     QAPPEGPLVLGVDAGSTTTKAILLDPSTHAVVASYYGRTKGNPVAATRECLQALIEQV
                     GNRQIGLIGTTGSARELAGAYLGTEHVYNEISAHAAGATHFDPDVDTIFEIGGQDSKY
                     IYLRNGVPIDYAMNNACSAGTGSFLEESAHSDLGITVSDIADLALAAPSPVNFKATCA
                     AFINSDIRLAQQQGHPRDNIIAGLVYAIAGNYLNRVKGPRYVGKKIFLQGGVALNRAV
                     GYAFAHSVGRQVVIPPSPELLGALGVGLLALKRSGEVLGHTVDLLTLGAPEMKLVGRF
                     TCGACKMYCSIDRFEVAGRRFPFGGRCSLYENVWKRKARTSAAQDLVEKRAEILFQVT
                     PAPPASTPKHERLERTHWGVGPAYESAKAFVRESLGRSNGTAVASGTRIGIPRALTTH
                     SLFPLYSTFFSRIGMEVVLSDVDPRGDLSSHSGFCFPAQIAHGAILDLVKKDVDLIFM
                     PHVVRMPHPNQCKDSYLCPITQAGPYFLAKAFPDRRLVSPVLEFINGYETCTTLVDMA
                     VAEFGIGRELATSAWEAAVQAQTDAERRLRDLGKQALDEAIAAGKPAILLAGHSYNAF
                     SPEASQSVGKKLSSMGVSVIPADCLLSSGEGPTSWHFANQILNAVGIAKQHHNLFLLS
                     VSNFSCTIDAFTHSMLASEMGSKPYLVLEIDAHTADAGVQTRLEAFLDIIHNYRDVHT
                     AGAGPFVRCRVVPGGRVIRSNGESVPLTDPRVKVYFPNFSPYHTTAFAMGARWLGLHA
                     GPVIPLDRTQLDKGLQHTSGRECLPLPVCIGQLLQIHEHRQPGEIAGYYMLQGGAPCV
                     SDAYMGYFERFLTEHRLTDLFLFNPQPDNDFLGFDKTVLAQHLSPTIILADILVEIDH
                     VLRVVGHEDALDQMRNEWERFADGARSLDEFHASLPSFVERLTHLPRTRDPRTCPRVV
                     VAGDFFTRFSSFFMDGVRDLYAERGIILKPVDLSELFLYVAYQPMAETATTWGMKPGG
                     LALAKACTRVFQPEGKEYIQQWMSYQTLKREEEHYRGLFRETGLLVAGPNAVSSIFEK
                     GAEHVSPAIYGELLPTVGKGLEAAREGYDGMLVIGPFNCLPFRISEAILKPLSIQQGM
                     PILTYESDGYAVSPSVLRQVDVHIQQVLEHATSNHVTQG"
     gene            complement(22604..23302)
                     /locus_tag="VLD60_02610"
     CDS             complement(22604..23302)
                     /locus_tag="VLD60_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HSB43871.1"
                     /translation="MKDDEATMRIPGLLPLLLFLVLLAMLPFVFGHLFAAALIKLKLE
                     PTTALLVVIGIFMGSPINIPVKRIPRTESMVADPLAVFGLPGRWPSTMRVRRETVIAI
                     NVGGCLIPVALAVYETAHLMAAGWQPLSGLLVAIFINTALCYWMAKPIEGVGIAMPGL
                     FPALVAAMSALFLVPDQAVPVAFVAGVLGPLIGADLLHLRDIEKLATGIASIGGAGTF
                     DGIVLSGIVAAYLA"
     gene            complement(23299..23814)
                     /locus_tag="VLD60_02615"
     CDS             complement(23299..23814)
                     /locus_tag="VLD60_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HSB43872.1"
                     /translation="MNDLQPAKVPVTRFMADAMLGRLARWLRILGYDTAYEKVVTDDV
                     LIERALRENRWLLTRDRRLAQRKLLRGRHTLIASDDVEGQLRQLRRDLTIDLDVDHQR
                     GYRCADCNVVLTSISHDDAAPLVPPFVSQQYREFLQCPHCRRAFWAGTHWDDLDRRLT
                     AIRDGSMDDRA"
     gene            complement(23807..24892)
                     /gene="rtcA"
                     /locus_tag="VLD60_02620"
     CDS             complement(23807..24892)
                     /gene="rtcA"
                     /locus_tag="VLD60_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HSB43873.1"
                     /translation="MLTIDGSRYSGSGSIVRQAVAFSALTGQAIHVVNARAKREKPGL
                     RPQHIRVVEAIAELVNGSAEGLASGSQDFTFRPGSLKSARHYHWDIGSAGSTTMLGLG
                     VLPVLAFSAAPVTVELRGGLFQDFAPSAFHLQYVLFPLLQRMNLCAEVEIVRPGYVPR
                     GEGILRFVVKPLTEPFRAIAQEKAGPVTRVWGISLSSHLEERQVSQRMADMARDILAK
                     AGHQVEIEVRNDTESLQRGAALALFADEGEAVRLGADQAGALRRRAESIGTHVAKQLL
                     DDLMSGATLDRFAADQIIPFAALAAGESRFIIPTVTDHVLTGAWLAELFLEARVRIDG
                     QRLIINGVGFWPNRTRAPGEQKRCSNE"
     gene            24989..>25536
                     /gene="amrS"
                     /locus_tag="VLD60_02625"
     CDS             24989..>25536
                     /gene="amrS"
                     /locus_tag="VLD60_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSB43874.1"
                     /translation="MGSKSAMIVPTKYWHQLKDGRIQCDLCPRSCTLQEGQQGLCFVR
                     ARQQNQIVLTTYGRSSGFCVDPIEKKPLNHFLPGTSVLSFGTAGCNLSCKFCQNWDMS
                     KSREMDTLADEASPETIARAAAKLGCRSVAYTYNDPIIFHEYAIDVAQACREHGIKSV
                     AVTAGYVCDEPREEFYRFMDAA"
BASE COUNT         5358 a         7076 c         7864 g         5238 t
ORIGIN      
        1 ctgggggtgc gctgggcaag acggatatcg agttgtttcc gcgtgctcag gccgagcagt
       61 ttcagactca tgatcgacag gtcctcgaat cgggagtggc caaagagttt gaagaggtcg
      121 cgtggcaggc cgacggcttc catacgagca tcgtcgtgaa attccctttg cgaaatgcgt
      181 ccggccagat ccacgcaatc ggcggcgtta ccacggacat cacggaccgc aagcgaactg
      241 aggaggcctt gcgtgagagt caccaggctc ttcgtcgaag ccaggaggat ctcagggaat
      301 tggcaggatg tcttctgtcg gctcaggatg atgaacggcg gcggctcgcc cgggatcttc
      361 atgatgacat gaaccaacgg ctggcagcac tggcccttga tctcgaagcg ttggcccgat
      421 ccctccctcc ctcgctgatc gaagcgcggc accaacttgc gtctgttcaa catcaggtcg
      481 tgaccatatc tgacgatgtc catgacctgg cgtatcaact gcatccttct ttgctggatg
      541 atgtcggcct tgaggtggcc ttgcaggatc tcgtgaggag ttttggacaa cgagagaaca
      601 tttctgttct ctacaacccg cagaacctgc cggactcaat tccaacgcca attgccggtt
      661 gcttgtaccg ggtgacccag gagagtcttc gcaatgcggc caagcatggg aaggcttctc
      721 aagtcgtcgt cgatctcttc ggatctccgg agggtcttga cctgtctgtg aaggataacg
      781 ggaaagggtt cacggccgag gaggggaagc gtcctcctca tcgaggcctt ggattgatca
      841 gcatgcagga gcgattgcgt ctcgtgaacg gggtattcac cgtggagtca cgaccgggac
      901 agggcacgaa gatttgtgcc tgggtgccgg tccgggagga atgatcgtgg cgcgaccacg
      961 tattctgatg gcggatgatc attcgatcgt gttagccggc ctccgcaaac tcgtggagga
     1021 agaaggagac gtggtcggca cggtggagga cggacgcgca ttggtcgaag aggcgcagaa
     1081 gctgtgtccg gatattatct tattggacat ctcgatgccg ctgctgaacg gcctggatgc
     1141 cgcccgccag ctcacgaagc ttgttccgga aagcaagatc gtcttcctga ccatgcacgc
     1201 gactccgact tatgccacgg aagcgttcaa ggccggcgca tcaggctact tgatcaaacg
     1261 gtccgcggcc acggagctca aggacgccat ccatgccgtt atgaaagggc aacactatct
     1321 gacccccttg attaccaagg atgtcttgga cgccaccctg catccgccga gggggcatgc
     1381 ccgtcggcgg cctccggcga gtttgacagc gcgccagcgc gaggtgctcc agctcattgc
     1441 cgaaggaaag gggatgaaag atattgcctc cgtcctgaac atctctgtaa agacagtcga
     1501 attccataaa ttccgtatca tggaggagct ggatctccat tcgaccgccg ccctgacaaa
     1561 atacgccatc gccgaagggc tcgtcagtct ctaaagggcc ccctagtagc attgctttga
     1621 tcttctggca cattccctag ttctctcctt tcatcggttt cactaagatc cctcccgaag
     1681 acagaaagat gacggccttt gttgtggagg tgtcccatgg ctgtcgaagg catgattcaa
     1741 gaagtgcacg atgcgctgga taagttcggc gcagcttgtc ccatggaaga agcggtggct
     1801 ctctgtccga acttgacctg gaatcaggta ttcctggcca tcgattacct gagtcgaacc
     1861 ggacaggttc gtgttctaca ggacccggcg aggacctatc gtgtgcaggt tcttcacccg
     1921 accggctcgg ccaaatcatt tcctgccacg gctcctttag caggagttgg ctccggaaag
     1981 tagaaaagta cggggttgct cgacgaatct tctctcacac aggccgggcc agagggtttg
     2041 tctcattttg aaatcgagtg ggtagacctt caagtaagga gacggcggca atagctagga
     2101 ggaatgatct gtcgtataga gaagggggaa ccccatgtca ccgacaatac tggctcaaag
     2161 gacagaggca ccgttagcgg ccgccacctg catccacggc cggatcatcg atgatgtgct
     2221 tggagaagac ggtgagagaa caggaaaggt ccgatgtgtc gagtgcgggg tcgtgttcga
     2281 cgaccctttt cgtggtccca attaagttta cgaggcctgc gcgttctccg ttcgtttcac
     2341 gtttgcgaca aatgttctgc tcagatgtaa tcgcgccttc cttttttgtg ctctcatcaa
     2401 acaataaacc ctgaactgcg ctccgcgagt tctgagccgg gagtcgtaca tcccgcgagg
     2461 gcctattggt atttcactaa gcagagcgtc gtcgcatggg gacgaaaatc ccatgttcac
     2521 agatcctgtt catcgcctct atcatgtgga catgcgtcag agtattcttg ccctgcaagc
     2581 ggccgttcct tacggggttt ttagccgtaa aagcctgttg tcaaccgtcg tgtcatggac
     2641 cagcttgcca aaactgtctg tcaggacatc atttactagc tcttcgatat cctccgtatc
     2701 gaaccaaaat tcatcgtcgg atccgcttcc attgagcgtc atgcggacca tgctttcatc
     2761 tgcaacgttc cgcgcctgga cgacaagcct cgtcttcgta gagatttcag tgaagccgac
     2821 ccgactcttc gcatggaccg acagatcgag aattttaccc gtgaggatga catctgccga
     2881 atcactactc cctcgcttgg tcacttgcca gcccctggcg gtgagatagt cgctgagggt
     2941 ttgggcgacg acctccatgg ggttaccgcc cgggacgtcg aaatagctga caccacccca
     3001 cagatgggtg cgaaccccca gaccggtctt ataggcgcga ccgtcctcaa tcggccctac
     3061 cgcgacacga agaggagccg ccggcttcga aaccgacccg gatccagtgt tgagtgggtg
     3121 gatctgaagc ggaataacct cgcctgttcc ggcgcaccca gccaaggcaa cgagactgac
     3181 gccgcaaatg agcagtcttc ttaattcctt cacatcaccc tccttacatg acaaggactc
     3241 ttaccgagcg ttgctctgac aggcctcttc gcccgcttac ctgttacctc gtgaaatgag
     3301 cgcgtcgatt tgactgccag caggcttcac tatggtcagt gcaaaagggg cgttccttcc
     3361 cgtaactcgt gatttggagc tgtgatgagg gaacgccaag atcctgcagg tactgtctcg
     3421 ctgcctgtgc acgccgttca cccaagacca gattgtatgc gctcgttccc cgctcatcgc
     3481 agtggccttc gatcaggatc ttggtgcctg gttgcatctt cagcacgccc gcattggctt
     3541 cgagcggagc tctggcatcg ccgcggatcg taaaccggtc gaaatcgaag aagacatcgg
     3601 agagctctgc gaccgggggc gccggttcaa tagccggtcg aggtgcgggt gcaggagcga
     3661 caggctgttc cacgatgcgc atgtcctcgg cagcaggagt tttggccgga ggaacttccg
     3721 ctttcggtgg ctcaactgtt gccgctgccg ggatttctgg ttttgccgga gctggagggg
     3781 cgaccttcgc ctcttccacg gcttgcgcct ttggggctgc tcccggtgtg aaactctggt
     3841 cttctaccgc cgtgacaacc ttccggcttg cgcaaccaga cagcatcacg acgaccagca
     3901 agcaaagggg aaaagtgaaa taggacaggc gataccgcat cgacatgagc gctccttggt
     3961 tatcggacgt gaatggaccg tgcaaccaaa ccatccggcg atttgagata cgttatgtcg
     4021 gctgattccc ctaccttcag gtcggccaac cggacagttt tcgtgcctct cgtgatcttg
     4081 gtatcagtcg ggacacgggc ccccaccgtc agctcttctt tattgggcag catgactttt
     4141 atcacaatcg tctgaggatc gacggtcgaa tttgtggcgg tcactgtgcc acgaaccatc
     4201 cgctggatcg tttctgctgt ggcgttcgtc atgggcgtgg tcaatgtcag gaatccactg
     4261 acgataataa gaccgagata cgacataata actttcatcg gaacagaccc tcctttgtag
     4321 agcgcagcct tcttccaggg tgtaacagag gaactcaagc tgtctctatg cgcaagaacg
     4381 ggaccacggt gcttcaaagc tgagcctttg ccaacttgta taaaagtctt gagaaagtcg
     4441 tgagttttag tggaaccgag gatggcccgg caagaggtgc ttgaggaaat ccgctacagg
     4501 tgcggcgacg gttctgtggc aaaaggtcgc catcatgtcc cgagaccaga atcgatttta
     4561 ggatttcctg catgattcca tctggtcgga attgttctgc aaatgaagac tcgagcgtaa
     4621 ggcggaagca ataggagtgt gtcggcaccg cgattgctca tcgaaggctg gatgggaggg
     4681 ctggagtatg cggatccggc tgtttccaca tgacgaatct tcgacgtgaa ggggatcgcc
     4741 atgtcgacac gccgtcccat ctttaacgtc cagaaacacc aggcttctca cctccactat
     4801 gacttccggc tggaaattgg cggagtgttg aaatcgtggg ccattcccaa agggccgtcg
     4861 cttgacccac aggtgaagcg gttggcgatt gaggttgaag atcacgatcc cgcctatgcg
     4921 gatttcgaag ggataatcga ggaagggcac tatggcgcag ggccggtcat tctctgggac
     4981 aggggctggt tcgaggcccg agaggaggga atttctcctg aagccatgtg gcgagcaggg
     5041 acgttggagt tcgtcctgca cggtcgacgg ctgcgggggg gctttgcgct catccggatg
     5101 aagggacgtc ccagccaatg gctgttgatc aaacgaagag acaaagaagc caggcctgac
     5161 agtgagatgc ccgaagaatg gaaccggtcg gttgtgagtg ggcgcaccat tgccgatctc
     5221 aaccgagcgg ctgaggcggg agagcttgag gcctatcgat gcggggccta atgacgcggg
     5281 atcagacgct gcacgacaat ggatctgagc atcgatggac gacgccatgg aatgtgctgg
     5341 ccacggccag gaatcacgag cacggacgtt tggccaggcg tcttaaacga ttcggcgatt
     5401 tccgctgggc tccctatctt ggtgtgttaa tcggacgagt ggaggatcac gaggcgtttt
     5461 tcgaacaatt acgccgcgcc ggagaggtga agccgggttt tctcttcccg ctcgccaggc
     5521 tcgtgccgct tgatcgcacg tttgtcttcg cgacggagac gttgatcccg ctgctcaagg
     5581 cggagttgct caccgctgcc gagcagatcg ggagcggatc gttttatatc cgaatcgaac
     5641 gcaggggaca caagggcgag attcacagcc gggagatcga gcaggaactg gcccatgcgt
     5701 tgatcgcatc gttggaagag cgtgggcgta ctccgcatgt cgacttccat gatcctgacg
     5761 tcattatcgc aatcgagatc atcggtgacg aatgtggggt ggggttcatc acgaagtcgt
     5821 tacgtgagcg gtacccgttc atcaaagtgc cgtaggcttg cgggaggcaa cggagaaagg
     5881 aggctcaatg acggtgtctc gtcatgtgga agaagcggca ggaaaactga aagaggcctc
     5941 gcggcagatt gcccttgcgc gcgaagggcc ggccacctgt gagaatcaga agatctggct
     6001 ggaagccctg acagcgtact gtgaggctct cgccgacatc cacacctaca ataatgagtc
     6061 gattcacgag aagctgcatg agttggcggg aaggacgggg ctcaggaagt ttcctgcttc
     6121 ataaaacatg aagcgaggtc ccgtttcggg acgtcaacgt tcgccgcttt accctcggcg
     6181 ctcgtctgat gactaacgca gttctgtcac acgcatgcgg gcctgatgga gggatgtgat
     6241 ggacgtcatg gaagccattt accagcgccg tgccgtgcgc gcctataccg accagccggt
     6301 cgacaaagcg actgttgaag tgctcatcaa ggctgcgacc catgctccca gctcaatgaa
     6361 cgagcagccc tgggcctttg tggtgcttca ggatccaaag cggctcgcat cctggtcgga
     6421 ccgtatcaaa gcccacgtac tactacgtct gaagccggat tcaccgttgg cgaagtatcg
     6481 agacatgatt gccgatcccg actaccatgt gttccaccgg gctgggacac tcattattat
     6541 ctgtgcgaga cagggtgcgc ataacggcga agaggattgt agcctcgccg cgcaaaatct
     6601 catgctggcc gcctgctcga tgggtttagg aacctgtccc atcgggtttg cgcgaccttg
     6661 gttgaatcag gccaggatca agcgaagcct cggcattcct gacggctatg tgccggtgtt
     6721 tcccgttgtc ttgggctatc caatcggaga gacgaagccg gtcgagagga gagcgccgga
     6781 aattctgttc tgggaatgag gtgggattgt gacgtcggga aaaccactgc aggtcgccat
     6841 cgtaggattt ggaagagtgg gacaggtgtg cgcagagttt atttccatga gtcacgacct
     6901 cagtctggcg gcgattgttc gtcggattgc gacggcaggg gggaagctcc cggaacaact
     6961 ccgacgcatt ccggtgatta cccgcatcgg ccaagtgaag gatgtccggg ccgcgctcat
     7021 ctgcgtgccg acaaatgtcg tcgaagaaac cgtctcccag attcttccgc atggaatccc
     7081 gattgtggag tgcgccacgc tacacggaga cgcgctccat gcccataagg aaatgatcca
     7141 caagctggca ctccgttatc aggtgccagc gatcatcgga gcggggtggg atcccggcgc
     7201 gctgtccata ttccgctcat ggtttgccct gctgacaccg ggaggggaga ccgagacgcg
     7261 ccatcacacg ggcatcagcc tccgccacgc caccatggct cgaagcgtga cgggagtgaa
     7321 ggatgctctc tgtgccgaag tgcggaccgc cgaaggaagg ttccaacgct atgtctatgt
     7381 cgaactggag aagggggcag acccagacaa gatcactcaa gctatccgcg ccgatccgtt
     7441 gtttctcgga gcggatacgc aggtgttttc cgtcgagagc cttgcagccc ttgaacaaga
     7501 agggcgggga gtggtgatcg atcgaagggg ccctccgcgc cgacttggac accagcatct
     7561 actgctggag gcgcgctttg aagagtcggt attgacggct caggtcatgc tcgccgccgc
     7621 tcgcgcagtc ccgcaacttg agccaggcgc ctactttctc agcgagatcc cgctcagtgc
     7681 aatgtggggg gagatgatgg agaaggctca ccgggactgg ctgtaggaat cgagcggtgc
     7741 tcaggtcgcc ccaacggagt gctccgcgac caactcggat ccggcgaagg agatgctgtc
     7801 atgatgaggc tatatgcggt cacgaagccg gaggagcaac agctggcctg ggaggccttt
     7861 ccctcctggg cgcattttac ctggctctat ctcctgagtg ccgtctctgc actgcggggc
     7921 gggttatttt tcaagtttgg tgtggacgga tgggagatgt gggtgattgg ggccgggctt
     7981 cttctcgtct gtgccgcgat attgcggcac tgggcacact atgagatttc gcaaggtcag
     8041 atcacggtgc ggaacggcta tacaaggcac gagattcaat ccatcccgtt gagcgatgta
     8101 ggagatgtgg aagttcagca ggggcgtatc gcagatttct ttggaatcgg cacgatcgtc
     8161 gttcatccgc gaagcaacga ccggttgctt tcgttacgag gcgtgaagga tccggaggag
     8221 atcatgattc gcatcaaagc cctggcgtgg aagcacaata gaacgggcag tcagcctcga
     8281 gtgagctcat gatggagcgg aaattgagtg gagaaggtcg gcgttgaatg gccgagagct
     8341 cttgcgcgca atagagcagg gtccatgagc gagacggtac aggagaggcc gtgaagatag
     8401 ccttgactgg tgatgtgatg ctcggtcggc tcgtggatca atacgtgatc caaaaccggt
     8461 ctgtgcgtcc cgaagctctc tggggcgacg tgctcccgat catgctggcc gccgattgcc
     8521 gactcatcaa tctggaatgt gtcatcagcg accggggaga accctggcat ccgaccacaa
     8581 aagcctttca cttccgtgcc cgcccccgcg cgctcgagtt tctccaggcg gccaagatcg
     8641 acggtgtgac cttggcgaac aaccatgtct tggatttcgg agccgatgcc cttctcgact
     8701 gtttgaacct gctcgaccgg acaggattta agcggaccgg cgccggtgca aagctggagg
     8761 aggcgcttgt tccggtgttg ctcaggttgc cggagggacg catcgcggtc gtcgcgctca
     8821 cggacaatga gccggaatgg gaggcgaccg atacgaagcc cggcgtcaac tacgtgacct
     8881 ataacgaccg cggattaaac gaaccctacc ggtcgcgggt ggctcaggtg ttgctctcag
     8941 cacggcggca agcagacctg gtgattgtca gtgcccacgt cggccccaat tggggcgcgc
     9001 cgtcacgagc gatgcaagcg ctggcgcgcg atcttctcga catgggagca gacctgtact
     9061 ggggccattc caaccatacg cctcagggga ttgaatgcta caacggaaaa gccattctct
     9121 actcggccgg cgatttcgtc gacgactatg cggtcgacaa ggatgagcgc aacgatctct
     9181 cgttcctgtt tatattggag gcggagcgag gccgtattgc gcgcatcagg cttcatccga
     9241 cctgcattga ggatcttggg gttcgtgtgg cgaatgaaca agagcggcag tttctcctgc
     9301 gaaccatgca gactaaatgc aaggtctttg gaaccacaat ggatgtagag aggcaggtcg
     9361 gaacaatcag cgtgaagtaa ccggtaacgc tcttagagtt ggcgttggca atctcctcgt
     9421 ttcgaaacta tggaagcaag ctgggaaaat tttccgcact aagccgatat gggtgtgcga
     9481 gggatttgcc cgaccaatga ggcggccttt gaaggagcag ggctcgcttt gaccgccatg
     9541 atcaccgacc tgggggctgt tgctccgaca caatcggtgc cgatcgcctg tgaggcgcct
     9601 gatgatgaac tgctgttggt cgattggctg aatgcgctgg tctatgaaat ggctccgcgc
     9661 aagatgttgt tcggccgcta tgggatggcg aagacccctc aagagggaag tagaaaagtg
     9721 ccaaacggca aatggctata agagggagga agggaatcat gctgccatta caggagttac
     9781 gcgtcaaact gctcgcacaa cgacgggatc tgtttcgtca ggccgcaaag acggaggagg
     9841 atctgctgtg gctcgagacg gatgtggaac gtgaagctga agagcgaggg cagacagaaa
     9901 ccatggtacg gttgctcgat cggttggatg agcgggaaaa gagggaaatt gaagccatcg
     9961 accttgctct cacgagaatc gaggccggtt cgtacggccg ttgtgagcgc tgcggcaagg
    10021 agattcccgt tgctcgactg caggccttac ccacggcgac gacctgcctg gcctgcggtg
    10081 aggcaggtga ggcgttgaag cgatgaaact tggtatgggg ccggattggc gagtgactgt
    10141 tcatgtggaa tgctccgcgg gacaccgagg cgaagaagtg ccccgggcgt tcattttggg
    10201 cgaccgtcga atcgatgtcg tagcggtcct cgattgctgg cctggccggg atcaccgcta
    10261 tttcaaggtt cggggtggtg acagtggtct gtacatcttg cggcacgata ctccacccga
    10321 cgagtgggaa atcaccttgt tcgagcagcg atgaaaccaa aacctgtcag cgcagcgtcc
    10381 aactagtttc gtgaatcggc cgaaggggcg gggggcttca ctcccgtcgc acaggcggtt
    10441 accttccgct gagatccacg cgtgtggtgt tgacgccact cgtgccggag cagcagtggt
    10501 atttctcccc agcgaggcat tgctgtttct gctggaaaga tacggaatac ctctacacgt
    10561 gtgaagcctc atgagaattg taggccacct tgatatggat gctttcttcg cggcgattga
    10621 ggagcgtgac aatccgttac tttgcggatt gcctattgtg gttggggctg atcctcgtgg
    10681 cgggaaaggc cggggcgtgg tatccaccgc gaattacaag gcacgcgagt acggaatcca
    10741 ttcggccttg ccgatctcaa tagcgtggcg gctgtcggaa gaagcgagga agcacggaca
    10801 acccccagcc acgttcctgc ccgtacgcat gcagcgttat gccgaagtct ccgcccatgt
    10861 catggagagc ctgcaacgtt ttgtattgct ggttgagcag gccagcatcg acgaagccta
    10921 tttcgatctg agttcgtgcg aatcctatac cctcgcagaa gaagtctgcc gcaaggtgaa
    10981 acgcgccata cgatctgaag agtatctgac cgcatcggtc ggcatcggcc ccaataagct
    11041 cgtcgcgaaa atcgcctccg gcatgtctaa acctgatggc ctgactaccg tgagagaaga
    11101 agacgttgcc gaattcctcg cgccccttcc tgtgcgaacc attcctggtg tcggccccaa
    11161 gactgagtcc gaactcgcgg cggtcggcgt cgagctgatc aaagacatgc gacggttctc
    11221 tcaggatgag ttacgcgcaa tgttcgggaa gcgaggaatg gatctgtatg agaaaattcg
    11281 tggcagggac gagtctccca ttgaggaaac gcatgaagtc aaatccatcg gggaacagga
    11341 aaccttcgag caggataccg ggaactcgcc gtttctggtc gagcggctca gggccctctg
    11401 tcgggatgtc atgcgccggc taactacaga agggtttacg cattttcacc gcgtggtgct
    11461 tacggtgcgc tttgccgatt ttgaaaccaa gtcacgcagt cacacgcttc ccgttcctat
    11521 gaactcgctc aatgtcctcc agggggaagc gatgaacctg ttgctgccct tccttgacga
    11581 tcgtgaaaac ccccagggga aattaattcg cctcatcgga gtccgtgtcg aaaagttgga
    11641 gcagttcggc gtccattctc agaccccgct cattgagtaa gtcggttgtc ggtcctgctc
    11701 tgcccctcca agccgcagta tttgtcgaag acctgcggtg atgtccctca gtgctgctgt
    11761 ccggacgagt cttgtggttg gcgggccctc ctatgccatt gccacagtag atagagcggt
    11821 gtcccggcta agaccgtcgc aataccgagg ccaccttcca gcggacgttc atagagcgca
    11881 accccgacga tcacggccga cacaagaaga taaaagcccg ggacaaatgg gtagagtggg
    11941 gtgcgatacg gacgaggcag gtccggttcc cgccgacgca gaatcggtac ggctcccact
    12001 gcaagtgcgc tgaagatcat cagcacagtg ccgctgtaga tcaccagtcg ttcgaaggtc
    12061 cccgacagaa tcagggctga ggcccacagg ctttgcagca gaatggcgtt gatcggggtg
    12121 cgttccatgc cgggggtgct cgcgaagagg gaaggaatca acccgtcttg cgccatggca
    12181 tagtagacgc gcggtccggc ccagaccatg gcgctcacgg ctcctgcgat cgaaaggcat
    12241 agcatgacgg tcacgaaccg gccggcctcg atacccagga gcgctctggc gactttatcg
    12301 gcgacgggaa gaatcggcgg ttcggccagt tccgtcaccg gcagcgcata gaagtagaat
    12361 ccgttcagca ggagatagag cagcgcgacg aagagcgtgc ctccgatcat ggtccggggg
    12421 atggtgcggg caggatcggt gatctctccc gccagatagg ccgcggcgtt ccaccccgag
    12481 taggcgtaga gggcaaagat caacgacacg acgtatggtc cgatgccggg gagaggagga
    12541 gtatccgtgg gagtgagatg agcccagtcg cccctcccga ccatcaggcc cgccgcgatc
    12601 agcaagagaa tagctcccac cttcaggacc gtcagcgtcc gttggagcca tccgcctgct
    12661 ccaacgccgg ccaggtggaa tcctgtgatg aaccagagga gggctaacgc gatggctgtt
    12721 gaccggatcc ctccttcgtc atcaagagga aggatctgaa agaggtaggc ggcgaagctc
    12781 acggctcctg ctgcaatggc cgcgctgaag ccgatggtga aggaggtcca gccgctgagg
    12841 aaaccgacga acggcccgta agcccggcgg aggtaggcat attctccacc ggcgaccgga
    12901 aaagcggcac ccaattcact gtaggagagt gctcctgcga gtgcgatgag tgcccctccc
    12961 agccaaatgg agaggatcaa gcccggatgg ccgaggtcgc gcgccatgaa tcccgtggtc
    13021 gtaaagatgc cgctgccgac gacgttgctg acgagcacac agccggctgt gaaccagccg
    13081 atgtgacggg gaagtgtgga tggatcttgc tcgcctcttt caatctgcat ggcctactct
    13141 tccttctctt ccccgaggcg ggtcagcggc acaaaccgca cgagcgtgag ggtcttgtgc
    13201 tcatagccct ccgtggttcg gcgatagagc tcgagggtct ggtagaatcg gccgaccggc
    13261 agaatcagcc gcccgccgac ggccaattga ttgagtaacg gctgagggac gtgatcgggc
    13321 gcggcggtca cgataatggc gtcgaaaggc gcctcctctg gccaaccttg gtagccgtcg
    13381 cccacgcgaa cccggacgtt gttgtagccc agttccgtca aggtgcgttc cgcttggcga
    13441 gccagcggct ccaggatctc gatcgtcagc accttgtcgg caagctccgc aaggacggcg
    13501 gcctggtatc cggatccagt tccaatctcc aataccttgt ccgtcttctt cagcgtcacc
    13561 acctccgtca tcatggcaac caacgagggt tgcgaaatgc tctggccatg gccgatcggc
    13621 aaggggccgt ctatgtaggc gaatgcagca tacatggccg gtacgaagcg atggcgcggt
    13681 acatgccgca tcgcggcgag cactgcttgg tctgtgatac ccctggggat gatgtgctcg
    13741 tcgaccatcc ggtggcgctc ggtcttgcga tttggatcga tgagaggaga gtcagctgct
    13801 cggtccccgt tgcatgagga ggcccccatc agcacctgga tgagggcgaa gcacaacggc
    13861 accagaggcg tggagcttcg gatgagagat ttcatggagg cctcggattt caactctcgc
    13921 agtgtcaaga gatgagcaat cagaatgcca cggcgtgagc ttgataatgt gactgaggag
    13981 atcgttgcga tgtcagtctg tagcgtttca ctacataggc agtcttaggg ctgtagcaag
    14041 ttatccctat gacgaggctg gttcttatga acttcgtgcg ccaagctgcc agatacactc
    14101 gggtttcacc agacaatccg aatggcaccg gccttgctcc aaacctgaga tctgacaatc
    14161 catgaagaac ggtgaccagt gatgagtgct gcgcgatgag ttgcttatga ctcagggttg
    14221 gatcatcccg ccgcagactg agccaaggaa aagccgtgac gagttgcaaa aggagcgcga
    14281 ggacaagatc cggcaactca tcgcggatgg cctgcttcgg tcggagagaa tccgatccgc
    14341 tctgctgacg gtccgccgcg aggattttat tccgcctcga tatcgcgact atgcctatca
    14401 agaagttccg ctcccgctcc ccggcacccg cgccacgatc tcttgccccc acagctatcc
    14461 cctattctat gaaccgctgg gactcgatgt gggccatcga tttttggaga tcggccttgg
    14521 ctcgggctac ggcgccgctg tggcgcggca agtcgtggga ccgaccggcg tggtggtgtc
    14581 gatcgaaatc gatccggtca cctatgagtt cggcaggacg aatcttgaac gggctggata
    14641 ccacgatgtg cgcgtggtgc ttggagacgg tggccagggg agccctgagc acgcgcccta
    14701 tgatcgcatc tgtatcacgg ccgcctgtgg agcggttccg cccccgctgc tggagcaact
    14761 cacggtggga gggaagctga ttgcaccgat cgaggaacag ggaatccaga acctcacgct
    14821 gctgactaaa ggtgaacagg gcctgaatcg ccaggtcatt tgcgaagtgt tgtacattcc
    14881 gttgagaggg cgatatggac aggttccctc cggttagaat gaggttgcga ccgttgcaag
    14941 agcaagagaa ggtagcatga tcgagcccat gataatcgag tcctcttgga tgtcccccgc
    15001 gcgggaagcg gtggagattg tggagcggaa gggattaggc catcccgaca ccatctgcga
    15061 tgccgtgatg gaatccgttg ccgtcgaact ggcacaggcc tatctcaaga cctgcggccg
    15121 ggtcctccat ttcaacgccg acaaggcgat gttggtggcc ggccaggtgg cctgccgatt
    15181 tggtggaggc tcggtgctcg ctccgatgcg gttggtcatg ggcgaccgag ccaccttcga
    15241 atggaaaaac aaacatattc cggtgaaaga gattgcggaa cgggcggcct ataaatggtt
    15301 caaacgtcat ttaccccgaa tcaaacccct caaagatctc gagtgccagg tcgagctgag
    15361 accagcgtcg gaggagctgc ggtccgtggt cgagcggccg cgggaagccg tggccaatga
    15421 cacttccgcc accgtggggt atgccccgct gacaccaacg gagcgcctcg tgttccaggt
    15481 cgagcagttt ctgaacggcc cctcgtttaa gaaggagttt ccggacacgg gacaagacgt
    15541 gaaggtgctc ggcgttcgga ccggcaggga cgtgaatatt acagtggcca tgccgtttct
    15601 ggctccactg atccgcacgg agtctgagta cttcaagcgg aaagcgtcgg caagacaggc
    15661 cctgaccgct ttcgtgaaac ggaagggagt ggccggtttc tctccgcagg ttttcttgaa
    15721 cgagttggat cagtccggcg ctggtgaggc cggcgcgtat ctgacgctgc tgggtacgtc
    15781 ggcggaagcg ggcgattcgg gcgaggtcgg gagaggcaat cgcgtgtgcg ggatcatctc
    15841 actgcgtcgg ccggccagcg ccgaggcagc agcgggaaaa aatcccgtgg ctcatgtcgg
    15901 gaaaatctac aacgtgctcg cgcaagtgtt ggcggaggac atccacaaaa aggtgcaagg
    15961 gctgcaagat gtgactgtct ggatcacaag ccagatcggc aagccgattt cctcgcctca
    16021 agtcgtggtg gtcgaagtca ccctggcgaa tgggacaacg ctggcggtcg tgcgcccgca
    16081 gatccagcga gaagtccggc tagccttcac ccacatcaag tccttctgca aggcgttgac
    16141 acggggagtg tatgcggtgt gctgagagcc tcgctaattc tgcgtcttgc tagactcgcc
    16201 gatagactcc ctcattttct gaccacggga tcaggccgaa ttcaccctca ggcaacgatt
    16261 cgccattacc aatcacgatg gcaccgcctg gtcgaatccg ttccgtgagt tgtcgaaggg
    16321 tggtgtcctg caacgttgca tcgaagtagg tgaaggccaa gtaccggcac aggatgaggt
    16381 caaaggtctc ctctggtgcc gcctttcgga tgtcctggac gagaaaggtc actggctccc
    16441 taaactccgg cttgatccgg aatccttccg ctgtgtgatc aaacgcctgc gcgcgcaagc
    16501 tctccggcag atcccgtagg ctgctcagcg ggtaacaacc ttgctgggca cgggcgatgg
    16561 cttgatcgtc gatgtcggtc gcgaggatga tgagccgaac ggtcgggaat tgggcttgga
    16621 gcttcagctt ccagagaagt gagagactat agggttcttc tccggccgcg cagccgaggc
    16681 tccagcatcg caagacgctc tcgccttggg ccagtgcctg ccgggcaagg gaaggcaaca
    16741 cttcttgctc aagaaattga aagacgctct tatcccggta aaaccgcgag atgggcatcc
    16801 agcaaaatga gtccaggacg gtccattctt ccgggtgggt ttccaaataa acctgatagt
    16861 cggcgagaga tggaagatcc agcagctgaa gccgtcgctg gatgcgctta tacacctgcc
    16921 gtcgcacttt gcgataaccg ggccatatga gccgcagcct cggcaggcac cattgaagga
    16981 attgaacacc ttctgcatcc ctcatcgctc tgcctgtgac cgctgcgctc cgaccagctt
    17041 gtacacgctc tccggttatc tcgtcccttc agggagacta ttcttcaact cggcagcgag
    17101 ctcgctcagg agccggtcac gattctccct cgtaacggtg atgagtctgc agtctggacg
    17161 atctttcacg tgacggatga acccttcgcc cttcagggcg attgtcgcaa cgagaaactt
    17221 cggctgattc agcagcgcag tgatgtcgtc cgtgaagcgg cgcgagaaac attccatctt
    17281 cccaatttcg tcgataataa cgagtcgaga ctgagtgtgg ctgaggtcaa ggtcagacaa
    17341 aagctgctcg aacccgtgga catccactcc gtatcggctg acacggtaag gcccggggtg
    17401 gtggatgtgg gacaagacca gctggtgacc gcagagcgtc acgagtcgga acccttctct
    17461 ggttccctga gcgtcacgga tttcttccgt atagaatccc gccgggtgat ggtctgccag
    17521 ttgccttgcc agctcacgag tcagcgtggt cttgccgact cccggtgttc cggtaatcag
    17581 aatgtgaggt gcacggccga taatcgtact ggtgtgaccg cttccggaaa tgtccgcggt
    17641 gtggcgttcg gagcccgctt gcaccgagtg ctccctcacg attttcgctt gtggattgtg
    17701 atgtgattgc cgtctggatc ctcgatcacc gccatccggc agacaggcgt gtcgaaggcc
    17761 tctgtgacaa acgccacggc ccgctccttc atcttctgca cgaaggcgtc gaagtctgac
    17821 acttcaaatg ccaccactgc tccctttgct cccgcagtat gtcccgtttc tgcggtggag
    17881 atggcgaaag tggaagcgcc aaggtcgtac tccatccaga tatcttgata gtgccaactc
    17941 acgtgcaggc ccagcacatg ttcatagaag gctcgagccc gttccatgtt ggaaaccgga
    18001 tagacggtga aagcgattga agtgatcatg gaaggattcc ttcttcggcg atgcgacgct
    18061 cggctcagcc atcgcaccgc cggcgagcac gagcgagccg cgcattaccc ttgtgtcacg
    18121 tggttgctgg tcgcatgttc gagcacctgt tggatgtgga cgtctacctg cctgagcact
    18181 gacggggaga cggcatagcc gtcgctctcg taggtgagaa tgggcatgcc ttgctggatg
    18241 ctgagcggtt tgaggatagc ttccgaaatc cggaagggga gacagttgaa cggcccgata
    18301 acgagcatgc cgtcgtaccc ctcacgggcg gcctccagtc ccttgcccac cgtcgggagg
    18361 agttccccat aaattgcggg cgagacatgc tcggcgccct tttcgaaaat ggacgaaacg
    18421 gcgttgggac cggccaccaa tagaccggtc tcacgaaaga gcccgcggta atgctcctcc
    18481 tcacgcttca aggtctggta gctcatccac tgttggatgt actctttgcc ttccggttga
    18541 aataccctcg tgcaggcctt ggccagagcc aggccgcctg gtttcatacc ccaggtagtg
    18601 gccgtttccg ccatgggttg atacgcgaca tagagaaaca gctcgctcag gtcgaccggc
    18661 ttgaggatga tgcctcgttc ggcatagagg tcgcgcacgc catccatgaa aaacgaactg
    18721 aagcgcgtga agaagtcgcc ggccacgacg acgcgtgggc aggtgcgcgg atctctggtg
    18781 cggggcaggt gcgtcaggcg ctcgacgaat gaaggcaggg aggcatggaa ttcgtcgagc
    18841 gaacgagccc catcggcgaa ccgctcccat tcgttccgca tttgatcgag cgcgtcctca
    18901 tgtccgacga cacggagcac atgatcgatc tccaccagaa tgtccgcgag gatgatcgtc
    18961 ggtgacagat gctgcgccag gacagtttta tcgaagccga ggaagtcgtt atccggctgc
    19021 ggattgaaaa ggaacagatc agtcagccgg tgctcggtga ggaatcgttc aaaatacccc
    19081 atgtaggcgt cgctgacaca gggcgcgccg ccctggagca tgtagtatcc cgcgatttct
    19141 ccgggctggc gatgctcatg gatctggagt agttgaccga tgcagaccgg caagggaaga
    19201 cattcgcggc cggaggtatg ctgcagcccc ttgtcgagtt gggttcggtc cagtggaatg
    19261 acggggccag cgtggagccc gagccatctt gctcccatgg caaaggctgt ggtgtggtag
    19321 ggggagaaat tcggaaagta gaccttcact cgtgggtccg tgagcggcac ggactcgccg
    19381 tttgatcgga tgacgcggcc gcccggaacg acccggcacc gaacgaaggg gccggcgccg
    19441 gcggtgtgca cgtcccgata attgtggatg atgtccagga aggcctccag cctggtttga
    19501 actcccgcat cggcggtgtg ggcgtcgatc tcgaggacca ggtagggttt cgaccccatc
    19561 tctgaagcga gcatggagtg ggtgaacgcg tcgatggtgc aactgaaatt gctgacgctc
    19621 agcaggaata agttgtgatg ttgcttcgca atgccgacgg cattcaagat ctggttcgca
    19681 aagtgccagg acgtgggccc ctctccagat gacagcagac agtcggccgg gatgacagac
    19741 acccccatgc tggagagttt cttacccacg gattgcgacg cctccggaga gaaggcgttg
    19801 tagctgtggc ccgcgagtag aatggccggc ttgcccgccg cgatcgcttc atcaagcgcc
    19861 tgcttcccga gatcacggag cctgcgctct gcgtctgtct gcgcctgcac agcggcttcc
    19921 caggcgctgg tggccaactc gcgcccgatt ccaaactccg ccaccgccat gtcgaccagg
    19981 gtggtgcagg tctcgtagcc gttgatgaac tccaatacgg gggaaactaa gcggcgatcg
    20041 ggaaaggcct tggccaggaa atagggcccg gcctgcgtga tcggacagag gtacgaatct
    20101 ttgcactggt tcggatgggg catgcgcacg acgtgcggca tgaagatcag gtccacgtct
    20161 ttcttgacca ggtccaagat cgcgccgtgc gcaatctgcg cggggaagca aaaacccgaa
    20221 tgcgaactca gatcccctcg tgggtccaca tccgagagca caacctccat gcctatacga
    20281 gaaaaaaacg tggagtagag cggaaacagc gaatgcgtcg tcagggctct gggaatgccg
    20341 atgcgagtgc cactcgcgac tgctgtgcca ttggaacggc ccaatgactc ccgcacaaag
    20401 gcctttgcgc tttcataggc tggtcccacg ccccagtgag tgcgttccag tcgctcgtgc
    20461 tttggcgtgg aggcgggcgg agcgggggtg acttgaaaga gtatctcggc gcgtttttcg
    20521 accaagtctt gagccgcgga ggtcctggcc tttcgcttcc acacattctc atagaggctg
    20581 cagcggcctc cgaaaggaaa ccggcgtccg gcgacttcga agcggtcgat gctgcagtac
    20641 atcttgcagg cgccacatgt gaagcggccg acgagcttca tctccggtgc tccgagagtg
    20701 agcagatcaa cggtatggcc aagcacctcg ccggatcgtt tcagagcgag aagtccgacc
    20761 cccagcgcgc cgagcaattc cggactgggc ggaatcacca cctgacgacc gacgctgtgg
    20821 gcaaatgcgt acccgacggc gcggttcagt gcgacgccgc cttgcaaaaa gattttcttt
    20881 cccacgtagc gcggtccctt gacccgattc agataattgc cggcaatggc atacaccagt
    20941 ccggcgatga tgttgtcgcg gggatggccc tgttgctggg cgagacgaat atcggaattg
    21001 atgaaggccg cgcaggtcgc cttgaagttg accggggacg gagcggccag cgccaggtcg
    21061 gcgatgtccg agacggtgat gccgagatcg ctgtgggcgc tctcttccag gaaggatccc
    21121 gtgcctgccg aacaagcgtt attcatcgca tagtcgatgg gcacgccgtt gcgcagatag
    21181 atgtacttgg agtcctgccc gccgatctca aaaatcgtgt ccacgtcagg atcgaaatgc
    21241 gtcgcgccgg ctgcatgagc cgagatctcg ttgtagacat gctcggtgcc cagataggcg
    21301 ccggcgagtt cccgggccga tccggttgtg ccgatcaacc cgatctggcg attgcccact
    21361 tgttcgatga gcgcctggag acattcccgc gtggccgcca cgggattccc cttcgtgcgc
    21421 ccgtaatacg aggccacgac ggcgtgcgtc gaggggtcga gcaaaatagc tttagtggtt
    21481 gtcgatcccg catccactcc cagaaccaac ggcccttcgg gaggagcctg atggggcggg
    21541 gccgcgatca cttgcacccg ttcgccatag agatggagcg gcgggagacg gccgaggccc
    21601 ggctgtgctg caatcttcgg ggatgtgtcg cagggtgagt ctcggacgag gagggcggtg
    21661 ccccaggctt cgaaccacga actctccggc agcaccacga attccgtggc cgggagcttg
    21721 gcgcgcaacg acgccaacat ggcttcatta cgcgtcacgc cgccgatcag caggactcga
    21781 ttcaggttgc gcgtgccttt ctcgagcaac gcgatgactt tgttggacat gctgtcatgc
    21841 aacgtgtgga gaatatcctc aggcgtggct tcgttgcggt tcagcttgtg ggtgatgtcg
    21901 gatttgcaat gcaccgagca gcgtgacgcc agcggcacga cctttccgct gaaggagcgt
    21961 tgaattgctt cctccatgcc cagccccatc cggccgatct gctggacgaa aaactcgccg
    22021 ctgcctgccg cgcacttgtt gtgggaaacg acattcgtga tcctcccttc catgaggagg
    22081 tagacgagga aggattcgcc gccgagcgac gcgacggcgt caaacgtgcc gtccacctcg
    22141 cggagcgccc gctgaaccgc ggccacttcg gaaatatgcc cgagctggcc ggatacgccg
    22201 aagtactcag catcggcgaa ttccgccctg gcgagcacct cgtcgagtat ctcgagcggc
    22261 cggccctgat gggccagcac ggtggcgctc ctggcgtcac cccggagtgc tgccaccttc
    22321 acggtgaggg cgccgatatt cacgccgacg taggacatac gaactccttc tgccagctct
    22381 caagtcagga agaagaggag caatgactgt gccttcttgt cgatagcgag caggcaggag
    22441 agggtctcga attgttgagt tttctcatcg aatgatgatg acgttgagat gtcactgtag
    22501 ccttcttacc caatccattt agagttgatg gggcaatccg cttcttcttt cttagagcaa
    22561 tagggtaact gaattgtgtt tgcaaggctg aaagagccgt tgctcaggcc aagtacgccg
    22621 cgacgatccc tgacagcacg atcccatcga acgtgccggc gccgccgatg ctggcaatgc
    22681 cggtggcaag tttttcgata tcacgtagat gaaggagatc cgcgccgatg agcgggccga
    22741 gcacaccggc gacaaaggcg acggggacgg cttggtcagg aacaaggaat aaggcgctca
    22801 tggcagccac caaggcagga aaaagaccag gcatggcaat gccgactccc tcgatgggct
    22861 ttgccatcca atagcacagc gccgtgttga tgaagattgc aaccagcaat ccggacagcg
    22921 gttgccaacc tgccgccatc agatgagccg tttcatagac tgccagcgcc accggaatga
    22981 ggcagccgcc gacattgata gcgatgacgg tttctcgacg tacgcgcatg gtcgagggcc
    23041 accggcccgg caatccgaac accgccagcg ggtctgccac catcgattcc gttcgaggaa
    23101 tccgcttcac agggatgttg atcggacttc ccatgaagat gccgatcacc acgagcaggg
    23161 cggttgtcgg ttccagcttg agcttgataa gggccgcagc aaacaggtgg ccaaagacga
    23221 acgggagcat cgccaacagc acgaggaaga gcagcagcgg cagtagtcct ggaattctca
    23281 tcgtcgcctc gtcgtctttc atgcgcggtc gtccatgctg ccatctctga tggctgtgag
    23341 ccggcgatcg aggtcgtccc agtgggtccc ggcccaaaag gcgcggcgac agtgcgggca
    23401 ttgaagaaat tctcgatact gctgcgagac aaagggcggg acgaggggag cggcatcgtc
    23461 atgtgagatg gatgtgagga cgacattgca gtctgcgcag cgataccctc gttggtggtc
    23521 cacgtcaaga tcgatcgtga ggtctcggcg gagttggcgc agctgacctt cgacatcatc
    23581 gcttgcgatc aacgtgtgac gaccgcggag gagcttgcgt tgcgccagac gcctatcgcg
    23641 cgtgagcagc caccggtttt cgcggagcgc gcgctcgatc agcacgtcgt ccgtgaccac
    23701 cttctcgtac gccgtgtcgt agccgagaat gcgcagccag cgcgcgaggc gaccgagcat
    23761 ggcatcggcc atgaaacgtg tgacggggac tttggccggt tgaaggtcat tcatttgagc
    23821 aacgcttctg ttcacccggc gcccgagtac gattcggcca gaatccgacc ccgttgatga
    23881 tcaatcgctg accatcgatc ctcacgcgag cctcaagaaa caattcagcc agccaggcgc
    23941 cggtcaggac gtgatcggtg acggtcggta tgatgaatcg gctttcgcct gctgccaagg
    24001 ctgcgaaggg aatgatctga tccgcagcga accggtccaa cgtcgcgccg gacatgagat
    24061 cgtcgagcaa ttgcttggcg acgtgtgtgc cgatcgattc cgcgcgtcgt ctcaatgccc
    24121 ctgcttggtc cgcgccgagt ctcacggctt ctccttcatc ggcaaagagg gccagtgcgg
    24181 cgccgcgctg cagagactct gtgtcgttac gaacctcgat ctcgacctga tgccccgctt
    24241 tggcgagtat gtctcgggcc atgtctgcca tccgctggct gacttgccgt tcttcgaggt
    24301 gagaggagag agagatgccc catactctgg tgactgggcc cgctttttcc tgcgcgatgg
    24361 ctcggaaagg ttcagtgagg ggtttcacga caaaacggag tatgccctca ccgcgaggca
    24421 cgtagccggg acgtacaatc tctacctccg cacagaggtt cattcgttgt agcaatggaa
    24481 acaggacata ctgcagatga aatgcagacg gcgcgaaatc ctggaagagg ccccctcgca
    24541 attcaaccgt gactggggct gccgagaagg ccaggaccgg caggactccc aggcccagca
    24601 tggtcgtcga gccggcagag ccgatgtccc aatgatagtg ccgggctgat ttgagagagc
    24661 ctgggcggaa cgtgaaatcc tgcgaacctg atgccagtcc ttccgcgctg ccgttgacga
    24721 gttcggcgat ggcctcgacg acacggatat gctgtgggcg taatccgggc ttctctcgct
    24781 tcgctcgggc attgaccaca tgaatggcct ggccggtgag ggcggaaaag gcgactgcct
    24841 gcctcacgat tgaaccactt ccggaatagc gggaaccgtc gatcgtcagc attgggaggc
    24901 gatatccgtt gaatggctca cgcaccctcg aaaggatctg ctggtataat gctcgcacac
    24961 caccgtgatc tcaagggagc ctgtcgtgat gggatcgaag tcagcgatga tcgtgccgac
    25021 gaaatattgg catcaactca aggatgggcg catccagtgc gatctctgtc cgcgctcctg
    25081 cacccttcag gaagggcaac aaggtctttg ctttgtccgg gcgcggcagc agaaccaaat
    25141 tgtcctgacc acctatggtc gatcgagcgg gttttgcgtg gatccgatcg aaaagaaacc
    25201 gctgaatcat tttcttcccg gcacctccgt gctgtcgttc ggcacagcag ggtgcaatct
    25261 ctcgtgcaag ttctgtcaga actgggacat gagcaagtca cgtgagatgg ataccttggc
    25321 cgatgaggcc tcgccggaaa cgatcgcgcg cgccgcagcg aaactcgggt gtcggtccgt
    25381 ggcgtacacc tataacgatc cgatcatctt tcatgaatat gccatcgatg tggcccaggc
    25441 ttgtcgcgag catggcatca aatcggtggc cgtcacggcg ggatacgtct gcgatgagcc
    25501 tcgggaagaa ttctatcgat tcatggatgc ggcgaa
//
LOCUS       DATIFR010000049         3204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1946112, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000049 DATIFR010000000
VERSION     DATIFR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946112"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1223)
                     /locus_tag="VLD60_02630"
     CDS             complement(138..1223)
                     /locus_tag="VLD60_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSB43875.1"
                     /translation="MARAAAAALPLTAHQRSELVQLTRLPSTPQSIVLRAKIVLGAAD
                     GVANKTLAARLVTTRMTVLLWRNRYAEEGLVGILEDRPRPGRPKEISPEQEAQIVEAT
                     RSTKPKHATHWTVRTLARGQGVSPASVYRIWRAYGLQPHRVETFKFSNDPQFVHKVRD
                     IVGLYMNPPDKALVLSVDEKSQIQALDRTQPILPLRPGLPERQTHDYVRHGTTTLFAA
                     LNVLDGTVIGQCQPRHRHQEFLKFLERLDGTIDRKRQIHLILDNYGTHKHPAVKQWFA
                     LHPRYQVHFTPTGASWLNQIERWFAEITSKRIRRGTFGSVKELERAIHSYIRENNHES
                     KPFVWTATAAGILRKLRKCKETSETGH"
     gene            complement(1383..3002)
                     /locus_tag="VLD60_02635"
     CDS             complement(1383..3002)
                     /locus_tag="VLD60_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43876.1"
                     /translation="MELFAKLFSSLLVFVYHCFDRIVIHGYLSGLSRPEQVVHFFRQV
                     LGLPVVSKEALSQRTNDYQNWVEAFARNHRLPIEWAEKGVRKEDYVLPWLRRMIKRDA
                     YGVYFIFKSMEQGPTFRISVPKYPTRDPNHRILAHQRSRFTHYYFYIRDEVLGPIVMR
                     VASFFPFQATYYLNGHSFIEQELNRRQIGFRKHDNAFLAVDDVAALQAAADRLSPQII
                     RQRLDYWTLILGPKFSKRERAQMNLSRFYAIAQIEYCRNFIFKRHFPIHKIFERSCEI
                     GLWRLTANRISEIFGVRLDKRLRGKLASVLDQIEHGHHVFRAYWKNAFLKQYEKFSAF
                     LRNELCSNNLRDFGLRKGLDHLDQVRKRFQTITDRFATFQAQWLNVHGDFPLLQRIAL
                     PITIGSVRYPGIKIHDARLIRLLEVLLHTGSTLGGWTAKQIHETVLTTFRLSPNAYGL
                     NQLRYDLRKLKGHGLLERDGSHYAYRLTPKGVQVALLFLFFHKRLCGPLANSRFHHRP
                     DPAHRSQGKLEAAYHKADKAIQDIVDLLAVA"
BASE COUNT          630 a          862 c         1007 g          705 t
ORIGIN      
        1 ttcggtaagc tctgtgctgg aaaacgactg gtggcgtcct ggcggaaccg cgtgatggga
       61 tatcctacga cacgatgacg cttgaagagc cgattttcct tcagaacctg ggccgcgtaa
      121 ccatcagttt cgctctgcta gtgtcccgtt tctgaagtct ctttacactt tctgagtttg
      181 cgtagtatgc ctgcggcggt ggctgtccag acgaacggct tggattcgtg attgttctcg
      241 cgaatgtagc tgtgaatcgc ccgttccagt tccttgacgc taccgaatgt cccgcggcgg
      301 atccgtttgc tggtgatctc ggcaaaccaa cgctcgatct ggttcaacca ggaggctcca
      361 gtgggcgtga agtgcacctg atagcgagga tgcagagcga accactgctt cactgccgga
      421 tgtttgtgag tcccgtaatt gtctaagatc aagtggattt gccgcttgcg gtcaatcgtg
      481 ccgtccagcc gttcgagaaa cttcaagaac tcctggtggc gatgccgcgg ctggcactgc
      541 ccaatgaccg tcccatccag cacattgaga gccgcgaaga gcgtcgtcgt accatgccga
      601 acgtagtcgt gggtttgccg ttccggcaac ccaggacgca ggggtagaat cggttgcgtc
      661 cgatccagcg cctggatctg gctcttctca tccacactca gcaccagcgc cttgtcgggc
      721 gggttcatgt ataaccccac gatgtcacgt accttgtgca cgaactgcgg gtcgttgctg
      781 aacttgaagg tctccacccg gtgcggctgt aacccgtacg cccgccagat gcgatacacg
      841 ctggcggggc taactccctg accgcgcgcc agggtccgga ccgtccagtg agttgcgtgc
      901 ttgggttttg tggaacgggt cgcctccacg atctgggctt cttgttccgg cgagatttcc
      961 ttggggcgcc ccggccgcgg ccggtcttcc aagatcccca ccaatccttc ctccgcgtat
     1021 cgattccgcc aaagcaacac cgtcattcga gtggtcacca accttgcagc cagcgtcttg
     1081 ttggcaaccc catcggctgc acccaagaca atcttggctc gcaggacgat gctctgcggc
     1141 gtgctgggta aacgagtcaa ctgcactaac tcggaacgtt ggtgggcagt cagtgggaga
     1201 gcggcggctg cagcgcgtgc catcctgctc tcagtatggc ctagcacacc ttaaatgtct
     1261 agctatttat gaaacatgac actagcctag cggaagtcgg gctagtgcaa ttcgaattgg
     1321 ctccttagat tcttacctcg aagatcgtaa acaggaatac tccaagattt gaacgggcag
     1381 ggtcaggcga ccgcgagcaa gtcgacgatg tcctggatgg ctttgtcagc cttgtgatat
     1441 gcggcttcga gtttgccctg ggaacgatgc gccgggtcgg gacgatggtg aaagcggctg
     1501 ttggccagag gaccgcagag gcgtttgtgg aagaagagga agaggagtgc cacctggacg
     1561 cctttggggg tgaggcggta ggcgtagtgc gaaccatccc gttccagcag gccgtggccc
     1621 ttcagtttgc gtaggtcgta acggagttgg ttcaggccgt aagcgttggg ggaaagccgg
     1681 aaggtggtaa ggaccgtttc gtggatctgt ttggcggtcc agccgccgag ggtgcttccg
     1741 gtgtgcagga ggacctccag caaccggatg agccgggcgt catggatctt gatgccggga
     1801 tagcgcaccg aaccgatggt gatcggcaag gcgattcgct gaagcagtgg gaagtccccg
     1861 tgaacattca accactgggc ctggaaggtg gcgaaacgat cggtgatggt ctggaatctt
     1921 ttgcgcacct ggtccaggtg gtccagacct ttcctcaggc cgaagtcgcg gaggttgttc
     1981 gaacacagtt cgttgcgcag gaaggcagag aacttctcgt attgtttgag gaaggcgttc
     2041 ttccagtagg cccggaaaac atggtggccg tgttcaatct ggtccagcac gctggcaagt
     2101 ttgccgcgga gccgcttatc gagccgcacg ccgaaaatct cgctgatccg gttggcggtc
     2161 aggcgccaga ggccgatctc gcagcttcgt tcgaagatct tgtggatggg gaagtggcgc
     2221 ttgaagatga agttgcggca gtactcgatc tgcgcgatgg cgtaaaagcg cgacaggttc
     2281 atctgcgcgc gctcccgctt ggagaacttc ggccccagga tcagcgtcca gtagtccagc
     2341 cgctggcgga tgatctgggg gctcaggcgg tcggcagcgg cttgcaaggc ggccacgtcg
     2401 tccaccgcca gaaacgcgtt gtcgtgcttg cggaagccaa tctgacggcg gttcaactcc
     2461 tgctcgatga aggaatggcc gttgaggtag taggtggcct ggaaggggaa gaaggatgcc
     2521 acccgcatca cgatcggtcc caagacctca tcgcgaatgt agaagtagta gtgggtgaaa
     2581 cggctccgct ggtgcgccag gatgcggtgg ttgggatcgc gggtcggata cttgggcacg
     2641 ctgatgcgga acgtcgggcc ttgttccatg ctcttgaaga tgaagtagac gccgtaagcg
     2701 tccctcttta tcatgcggcg cagccagggc aggacgtaat cctctttacg aactcccttt
     2761 tcggcccatt cgatcggaag tcgatggttg cgagcgaagg cctccaccca gttctggtag
     2821 tcgttggtgc gctggctcag cgcttccttg ctgaccacag gaagaccgag aacctggcgg
     2881 aagaaatgga ccacctgctc ggggcgggaa agcccgctca ggtagccgtg gatgacgatg
     2941 cggtcaaagc agtggtatac aaaaaccagc aggctggaaa acagctttgc gaacagctcc
     3001 atcggtcgtc tcccggatct cgtctcagca acaagatcat cggtcagacg atcgatggag
     3061 tcaaaatttc aaatatgttt ggctagagtt gtgcattaga aatactgtgc cttttgtctc
     3121 gtggggcgga cgatcgccgt tggtcgtctg ccaccctcgt agggcatgga agacgatggc
     3181 ttgcggcgcc ctctttccgc tgtt
//
LOCUS       DATIFR010000050        35595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2137832, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000050 DATIFR010000000
VERSION     DATIFR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 35595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35595
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137832"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="fabG"
                     /locus_tag="VLD60_02640"
     CDS             complement(<1..684)
                     /gene="fabG"
                     /locus_tag="VLD60_02640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSB43877.1"
                     /translation="MSLQGKVAIVTGAAQGIGRAIAESLAQAGADIVVADLDPTRSKE
                     TVAAVEQFGRKALNVKVNVADANDTKAMAEQVIKEWGKIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLQPMTKQRYGRIFNIASIVGAMGNVGQANYAASKAA
                     VIGFTKTVAREYASRNVTVNAVAPGFIDTAMTQGLSAEVKDALQKQIPLGRLGTPADI
                     ANAVRFLVSE"
     gene            complement(696..1643)
                     /gene="fabD"
                     /locus_tag="VLD60_02645"
     CDS             complement(696..1643)
                     /gene="fabD"
                     /locus_tag="VLD60_02645"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSB43878.1"
                     /translation="MNSGIGLVFPGQGSQSVGMGKALCGAHPHLKTVYEEAASVLGYD
                     VAQLCFEGPAEKLNLTEHTQPALLVSSMAALRALEPAGVKAIAVAGHSLGEYSALVAA
                     GGMGFRDAVGIVQKRGRYMSEAVAPGTGLVAALLGLSADAVKDVCREAGSVGVVQAAN
                     FNSPGQVVIAGEKAAVERAIEIARTKGCKKAVPLPVSVPVHTPLMQQAADRLAEDLAA
                     IRWSDLQMPLINNAEARTITKAEEIQPSLVRQLPSSVLWEDSIKAMAAMGVKTFIEVG
                     PGTVLTGLIRRIVPDAVTMNVNDPKSLDATRAALGAQDV"
     gene            complement(1771..2745)
                     /locus_tag="VLD60_02650"
     CDS             complement(1771..2745)
                     /locus_tag="VLD60_02650"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSB43879.1"
                     /translation="MRARITGTGSYAPAKVLANADLEKMVATSDEWIVERTGIRERRV
                     AGAGEACSDLALKAAQRALAAANIGAADLDMILLATCTGDYPLPATACLIQHQLGATR
                     AAACDLSAACCGFVYALSVADAYVRTGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAAVVSASEGNRGILSTHLRSDGALWELIAVPGGGSRLPPSEKVVAEKLNCIKMKGNE
                     TFKVAVRTLEEIARETLAANNVGIEDLDLYVPHQANLRILKAVADRLGLPLEKVMLNL
                     DRYGNTSAASIPIALDEAIRQGRIREGSLVMLGAFGAGLTWASALIRW"
     gene            complement(2742..3785)
                     /gene="plsX"
                     /locus_tag="VLD60_02655"
     CDS             complement(2742..3785)
                     /gene="plsX"
                     /locus_tag="VLD60_02655"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSB43880.1"
                     /translation="MTASSRTKIALDAMGGDHGPGPCIEGALQAAKELDVDVILVGDE
                     KLLSDECRRLGCTDERLSIKHAPQVVEMHESPAAVARKKRDSSIWIATELVKSGEAHA
                     VVSPGNTGASMVASFFVLGLTKGVERPAIATSLPTLTGEAIMLDVGANVDCTAQHLAQ
                     FAIMGNEYGKHLFRKVNPRVGLLSIGEEDSKGNEVTKEAFKLLKSTPLNFIGNVEGRD
                     VYSGEADVVVCDGFIGNVALKISEGVAETIKKLLIKEISGSFFGRLAYPLIASPLLNL
                     KRKIDYAEFGGAPLLGVNGITMICHGRSSAKAIKNAIRRAKGLAESRVDELIQRDIQE
                     NLGQSRPAEEPPA"
     gene            complement(3960..4145)
                     /gene="rpmF"
                     /locus_tag="VLD60_02660"
     CDS             complement(3960..4145)
                     /gene="rpmF"
                     /locus_tag="VLD60_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSB43881.1"
                     /translation="MPNPKHKHSRARRDKRRTQKLRVTPPGMSVCPQCHELKLPHYTC
                     LNCGTYKGKAVIQVEEV"
     gene            complement(4206..4811)
                     /locus_tag="VLD60_02665"
     CDS             complement(4206..4811)
                     /locus_tag="VLD60_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HSB43882.1"
                     /translation="MQVGATMELLTPLVADITAEGLSLVGEVTAEELSLTDADATLRG
                     PLSVSLDLMNADGMIAVTGVLEGTVVRQCVRCLKEYDDALAFSVHAAFAREGKESKGS
                     GRQPKAVEPRRGRPAPVKMEPDVEEEAEGDDRYFYQGDHVELAPMLREHIILAAPMQP
                     LCRVDCAGLCARCGEDLNAGPCQCPAEPPATAIRVIRTTKH"
     gene            complement(4833..5108)
                     /gene="rpsR"
                     /locus_tag="VLD60_02670"
     CDS             complement(4833..5108)
                     /gene="rpsR"
                     /locus_tag="VLD60_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSB43883.1"
                     /translation="MEREHGGGGRGESGGSGGGGRFFQRRRPCRFCIEKGSIDFKDVG
                     LLRNFLTERGRIVPRRISGNCLGHQRELTTAVKRARHIALISFAEER"
     gene            complement(5133..5543)
                     /locus_tag="VLD60_02675"
     CDS             complement(5133..5543)
                     /locus_tag="VLD60_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSB43884.1"
                     /translation="MAGFNKVILIGNLTRNPELRYTPNGTPVASMGLAVSRRYKQGDE
                     LKEEVCFVDVVVFGKQAEHCGQYLSKGNGIIVDGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFLPKRGDAGGGGGGGSEAPAHDEPHYDYEEPQ"
     gene            complement(5530..5877)
                     /gene="rpsF"
                     /locus_tag="VLD60_02680"
     CDS             complement(5530..5877)
                     /gene="rpsF"
                     /locus_tag="VLD60_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSB43885.1"
                     /translation="MELYESLFIIRPSITDEETNALIEKMKGVAGKTGAQFIKAENWG
                     RKKLAYEVRRERKGTYVYFYFKAPNNTVGELEHAYRLEDNIIKFLTVHLEKELVPRRV
                     AETPSEEFAGGRV"
     gene            complement(6073..7164)
                     /gene="ychF"
                     /locus_tag="VLD60_02685"
     CDS             complement(6073..7164)
                     /gene="ychF"
                     /locus_tag="VLD60_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSB43886.1"
                     /translation="MGLCCGMIGLPNVGKTTVFNALTSGGALAANYPFATVDPNTGIA
                     LVPDPRLLTLTDIFKSKKTTYSTLEVRDIAGLVEGASKGEGLGNQFLGHIREVDALLH
                     VVRCFQGTDVVHVSGGINPLRDIGVIETELMLADLEALDRRRQKTEKKVRAGDKKAAF
                     EVEFLTRLIGLLDKGEWLGNLTYQPEERALLDECQLLSAKPVLFVANVSEGKNADEAM
                     VKTVRDFAEKRGARVVTICGQLEAELSSLPESERSDFLKEMGLTESGLVRLTREAYTL
                     LNIITFFTAGEIESRAWPIVKNTKAPQAAGKIHSDMERGFIRAEVYHYDDLLACGSEA
                     KVKEKGLFRLEGKDYIIKEADIVYFRFNV"
     gene            complement(7170..7757)
                     /gene="pth"
                     /locus_tag="VLD60_02690"
     CDS             complement(7170..7757)
                     /gene="pth"
                     /locus_tag="VLD60_02690"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSB43887.1"
                     /translation="MRLIVGLGNPGASYAQTRHNVGMWVIERAAARWSIHLSRRGIAQ
                     RGSGRLGSELIELAGTLDFMNVTGPPLKGLLRELKLTADDLIVVHDDLDLEPGRLRIK
                     LAGGHGGHNGIKSVVETLGTPQFVRIKIGIGRPAPRQDSADYVLEPITREEMDIVEPC
                     LERAVDALECVIHRGTETAMNQFNVRDQSTEEKEG"
     gene            complement(7760..8545)
                     /locus_tag="VLD60_02695"
     CDS             complement(7760..8545)
                     /locus_tag="VLD60_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSB43888.1"
                     /translation="MKFDLAVNVREKSGKGAARQLRRNGKVPGVIYGQGECLLLTIDP
                     EPLVKILKSQAGSTALISLTITGAKSKPKRTALLRDYQVDPVDGQVLHADLFEISMEK
                     PIRVKVPVHVIGGTPVGVKEGGILHHNMRELHVECLPAALPDYIEVDASPLAIGQGIH
                     LKEIVKKEGLRYLDDSEQMVVSVAVPMSEAKLEALLTSAAVGPEGAKEPEVVAKGKAA
                     VEGAEGVAAAPGPAAPAGEAKAGEKKEAAAAPKAEKKEAEKKK"
     gene            complement(8595..9536)
                     /locus_tag="VLD60_02700"
     CDS             complement(8595..9536)
                     /locus_tag="VLD60_02700"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSB43889.1"
                     /translation="MSRELMIFSGNANPALAHEICAYLGVKLGEATVSSFSDGEIRVK
                     IEENVRGADVFVVQSSCEPVNDSLMELLIMIDALKRSSANRITAVIPYFGYARQDRKD
                     QPRVPISAKLVADLISTAGTDRVLTMDLHAGQIQGFFNVPVDHLYALPVLLDYITKKK
                     ISDLVIVSPDAGGVERARAFAKRLQASLAIIDKRREGPNQTQIMNIIGDIRGKSALLL
                     DDMIDTAGTIVQGAQACADKGAREVWTACTHAVLSGPALERLQNSCLTQVIATNTIPL
                     RGKEKICPKIHQLSVAPLLGEAIRRIHEDESVSSLFA"
     gene            complement(9741..10664)
                     /gene="ispE"
                     /locus_tag="VLD60_02705"
     CDS             complement(9741..10664)
                     /gene="ispE"
                     /locus_tag="VLD60_02705"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSB43890.1"
                     /translation="MTKSDSDSTTSPLSVVTVSAPAKINLVLRILDRRSDGYHYLWSI
                     MQTVALEDELQFRLAPHRNNIQLRCDTEQLAAGQSNLVYRAAAAVLERAPQATGLDIQ
                     LLKRIPMGAGLGGGSSDAAATILGLNRLFHLGWSIAQMAEVGQLLGSDVPFFLFSPSG
                     VVAGRGEAVKPIAIGERRWVVLVNPGFGVETKWAYQELAKTREAVRPLSSMHEDLDRQ
                     ACATWAQVVAAAENDFEAPVFARHESLRRIKQTLLAQGAELALLSGSGATVFGLFHDE
                     GTAHKAEAQFAQEKRLKVFVVPTCSGPLTVR"
     gene            complement(10657..12042)
                     /locus_tag="VLD60_02710"
     CDS             complement(10657..12042)
                     /locus_tag="VLD60_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB43891.1"
                     /translation="MEKILVVDDEKSLREVMSIMLKRAGYAVTEASDGEEAIGQVNKE
                     IYDLVITDLRMPKADGMEVLKAVKSSSPETVVLVVTAFATADSAVEAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLSTENILLKREMASQSSFARIVGQSEAMQKVFDVVRKVA
                     DAKSNVLICGESGTGKELVARAIHYNSGRSTMPFVAVNCSAVPETLLESELFGHMKGS
                     FTGAIANKAGLFEVANGGTIFLDEIGDTTPTIQVKLLRVIQEREFRRVGGNQDMKVDV
                     RVIAATNRDLEKAVADGSFREDLYYRLDVIPIRLPPLRLRTGDIPLLVKHFLEKFAQE
                     SGKAAPVLDQEAMHVLLQHEWRGNVRELENLIERVVAFSTGAPVTDAEIRGWLHPAVT
                     PQLSQGVPTDLPEDGLDLEALINGVEKDLLVKALERSKWVKKKAARLLKLNTRSFRYR
                     LEKYAIKGGRD"
     gene            complement(12027..13724)
                     /locus_tag="VLD60_02715"
     CDS             complement(12027..13724)
                     /locus_tag="VLD60_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB43892.1"
                     /translation="MDELRTRLHWLMGLRVVVVTLMLGLSLIFQATNGERVETFYALI
                     ILTYALTIPSALVLRRLNTAATLAAFFWIQLGIDFFLETVLVARTGGVESPFTVLYII
                     TVAVASLIPQRRVGLLAACGGIILFGLVTNIQLYGLADGWGWLQQSHLTAPEIFQTFG
                     SYALALLVVGLLSGTLADQLQQADQSLREKEQGLYRLQAFHENIVQSISSGVFTADAR
                     GAITSFNPAAQEIMGYALGQVIGRSWRELFNWHPNHASDDQQEDVPPSMTRFEVECKS
                     SRGNRLVLGMTVSPLHEQGEEGGLVGVFKDLTQIRDMEEEMRRKEWLANLGEMSAGMA
                     HEIRNPLGALAGAMQMLRKELSADETDRRLMDIAIREATRLDAIITEFLQYARPPALT
                     LTECDMNKILAETLDLVQHEARTRKNITIVTSLTGRPLLAQVDQDQLRQVFWNLATNA
                     FDAMPAGGQLTIATGCRRVDAGGRHGDVVEIAFQDTGDGIAKDNLDKIFLPFFTTKKE
                     GSGLGLAAVHRIVDLHGGWIKVESHLKQGTRFVVCMPRSGDNGVRLWHEGRTPWKRS"
     gene            complement(13945..15159)
                     /locus_tag="VLD60_02720"
     CDS             complement(13945..15159)
                     /locus_tag="VLD60_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB43893.1"
                     /translation="MATFAYVGRTKSGAVKKGELSAKTKDEAVDQLRKQSVVVTSLEE
                     KSGKGGFNLSLGSGLTDKDLVVFTRQFGTMINAGLPLVQCLEILSTQSENKVLRETIG
                     EVKTQVEAGSTFSDALRRYPKVFDDLYVNLVHAGEVGGLLDTILTRLAKYIEKAMKLK
                     GQIKSAMVYPSAILGVAAIVIMVLMIWVIPVFEKMFKEMSGGKMGLPGPTQLVIDISN
                     IFQSYWYVGLGIIVGCAMAIKKYYATPKGRMAIDKLLLKTPIFGDLIRKASVAKFTRT
                     LGTLLSSGVPLLDGLTICAKTSGNKVIEEALVNARVSISGGKTIADPLATSGVFPKMV
                     THMIAVGESTGALDAMLGKIADFYEDEVDQAVASLTSLLEPIMMVILGVIIGFIVIAM
                     YLPIFTMASAIG"
     gene            complement(15315..15851)
                     /locus_tag="VLD60_02725"
     CDS             complement(15315..15851)
                     /locus_tag="VLD60_02725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSB43894.1"
                     /translation="MNIPNSLTILRILLIPVYIGLLVYERFDQALIVLLVAGLTDALD
                     GTIARAANQRTRLGAFLDPLADKLLLTSGFITLSSIHLIPSWVTIVVVSRDAMLLLGT
                     AVAQFIESPINIAPTFLGKGTTFLQLSYVLLVIFLSSRHMDLAVLTPLLLGMMAFTVC
                     SGFHYLYRGYLHTGSSNS"
     gene            15995..16312
                     /locus_tag="VLD60_02730"
     CDS             15995..16312
                     /locus_tag="VLD60_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43895.1"
                     /translation="MSSLSSLFKKEGLVEKHQLEGVDPSDRYFNRAILVNRTPSGYAA
                     KVMYEALTVEGQSHPTISAAVKELVEKLQPLGFSRLRTRANFKGTKYLAEKETWVDYQ
                     DPA"
     gene            complement(16313..17455)
                     /locus_tag="VLD60_02735"
     CDS             complement(16313..17455)
                     /locus_tag="VLD60_02735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB43896.1"
                     /translation="MTEPDAIQTAMDQAVQDGVFPGAVLAVRLRGSIRCLVVSGRLSS
                     DPSGEPVQPSTVYDLASLTKPLVTVTSVLLLVQGGRLRLENAIQQHLSELEGAPIGLA
                     TVRDLLTHSAGLPGWRPLYERLESKGLEKGSGQPAVIRQAVLQLIRDEPLLYGRRERS
                     LYSDLGFILLGLAIERVTNLTLDQQFQRAVAEPLKAWPLLFGPLTAGTSEQAMTRSVH
                     PSKIAPTEWDEWRGRVLHGEVHDENAAAMGGVAGHAGLFGTAEAVLVLTGAWLRAHRK
                     QSSILDGELVRTFTKRQSDIPQSSWALGWDTPSTPSSSGSFFSPKSFGHLGYTGTSIW
                     IDPQQELEVVLLSNRVHPCRKNDMIRAFRPKIHDLVFKEFVSSGAK"
     gene            complement(17561..18073)
                     /locus_tag="VLD60_02740"
     CDS             complement(17561..18073)
                     /locus_tag="VLD60_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HSB43897.1"
                     /translation="MKITPLDIQQTVFKVSFRGYDKEEVNRFLEEIAETVELLNRDNA
                     VQQEKIVFLEQQLAELKRTEATLSNTLVSAQSLAEDVKRSAQRESELVIKEAELKAGE
                     MMRQARVELTNTQRDLSALQKQRLLMVERLRATLRTFERMLEVEEQEAFHDSATTPEH
                     KLEGESSPAL"
     gene            complement(18093..18389)
                     /locus_tag="VLD60_02745"
     CDS             complement(18093..18389)
                     /locus_tag="VLD60_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSB43898.1"
                     /translation="MFVMGNVLQGVATVLDTVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLERVTEPVLAPIRRWVGWRMGIDLSPIIAILIISFLQIALVRTLSDLARQMN"
     gene            complement(18389..19204)
                     /gene="proC"
                     /locus_tag="VLD60_02750"
     CDS             complement(18389..19204)
                     /gene="proC"
                     /locus_tag="VLD60_02750"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSB43899.1"
                     /translation="MSNMMAKKLAFIGGGQMAEAIIGGLLTGQLCKSESLWATDPVAA
                     RCDRLKSRFGIRVGSDNRQAVSWAEVVILAVKPQTMPDVLHEIGAALGRTLVISIAAG
                     VTIRSIGEQVSGARRIVRAMPNTPALVREGMTALAFSEGISEDDKGVARTVFEAVGRV
                     VPVEERLMDAVTGLSGSGPAYVFQAIEALADGGVKMGLPRQTAELLAAQTVLGAAKLV
                     LESGEHPAKLKDRVASPGGTTIAGLHQLEQGGFRATLMGAVEAATKRSQELGR"
     gene            complement(19197..19907)
                     /locus_tag="VLD60_02755"
     CDS             complement(19197..19907)
                     /locus_tag="VLD60_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSB43900.1"
                     /translation="MDSQTSGSIGERVSSVLDRIRSAEQASGRPKGSVRLVAATKAVS
                     SELIREAIAAGLTLAGENRLQEALPKIEALQGEPIKWHFIGQLQRRKARSVIGIFELI
                     HSVDSFDLAQEIDRRAEAAGLQQEILLEVNIGGEGSKAGFEPGKLEQTLSRFGTLTHV
                     AVKGLMAIPPPVEVAEQARSYFRALRELGRRLALQAIPRISMNELSMGMSNDYQVAIE
                     EGATLVRVGTAIFGARHV"
     gene            complement(19974..20708)
                     /gene="pgeF"
                     /locus_tag="VLD60_02760"
     CDS             complement(19974..20708)
                     /gene="pgeF"
                     /locus_tag="VLD60_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSB43901.1"
                     /translation="MAASVITVPAFASMRDGVQHFFGTRGHAETVSFDVGVPVPAGGS
                     RGRGWLLSVKQVHGTDALVLDRPLTEGDRFDGGWDALVTDQPGVTVAVRTADCVPVLV
                     YDRHRRAAAAIHAGWRGAVAGIVPRTIDLLISQFKSAEGDLRVSIGPSAGACCYEVDE
                     TVLAPLRSGWSDWPAVVQDDRGAKARLNLKELVRRQLTQIGIRPEHVTTVNLCTICHS
                     DLFYSYRREGRVNGTMLSGITLAAHR"
     gene            complement(20730..21911)
                     /gene="ftsZ"
                     /locus_tag="VLD60_02765"
     CDS             complement(20730..21911)
                     /gene="ftsZ"
                     /locus_tag="VLD60_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSB43902.1"
                     /translation="MFSFQEDVLSPVRIKVIGVGGAGCNAVNTMISSGLARVDFISAN
                     TDLQALDRSRAAYKVQLGPERTRGLGAGAKPEIGKESALESKDQIRECLEGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVGVVTKPFSYEGHRRMTHADEGIRDLRRHVDT
                     LLVIPNQRLLGIVDKATPLLEAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTVM
                     SHTGRAVMGMGVSRGTNRAIEAAQKAICSPLLEEGSVEGARGVLLNITGGPNMSLHEI
                     EEAASIIQQTADPEANIIVGQVINPDMGDDLVVTVIATGFERDEQPMPVAVAATRTAR
                     ATQQVLNGVGAAVAERPHKDLDRPTFLRRMGETRETMERMTAVADDEWDVPTFLRRQA
                     D"
     gene            complement(21962..23197)
                     /gene="ftsA"
                     /locus_tag="VLD60_02770"
     CDS             complement(21962..23197)
                     /gene="ftsA"
                     /locus_tag="VLD60_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSB43903.1"
                     /translation="MAKRDQILVGLDIGTTKICAIVSEVTEEGTLNIIGVGSSPSRGL
                     RKGVVVDIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISAENCKGVVALKKAEV
                     TRDDIQRAIESARTLAVIPHERRILHVLPREFMVDGQEGVREPLGLSGNRLEVNVHVI
                     TGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSQEERDLGVAMVDLGGGTTDLA
                     IFLDGSIRHSAVLPIGGQNLTKDLAIGLLTSQTEAEKIKVQHGLARTELVQGHETVEV
                     PSVGDRPARTFSRRDIAEILEPRVEEMFELVRREITRAGYEGMLGAGVVVTGGTSLLE
                     GMPDAAEHVLNLPARRGAPAGVGGLRDIVSNPMHATGVGLLLHARRHTEEMATAGLRS
                     GRPIGKVLDRMKSWMFEFF"
     gene            complement(23202..24071)
                     /locus_tag="VLD60_02775"
     CDS             complement(23202..24071)
                     /locus_tag="VLD60_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSB43904.1"
                     /translation="MKIPFRKRPTKAAGHRMNQWKGPRAMQAAELRQQVRRTQLAVKC
                     RRALRLTGWILGATIIVAVGVAGSRALGPVIQRGLEIRNVRVEGIHQVTIQDILNQLA
                     LKKGIALHQVSLSYLAERLQTHPWIKEATVERLPLHELRVTVLERKPAAVARIGSEHF
                     LTDDGGAVLARLGARDDATLPLLTGLDAKVLLQGDRHLRQTIQSSIELAKAMAQSVDG
                     RVEIELGNPVGLIASAKGVRFQFGSDALMDQWDRFTKVKAVSRATTFEGRKREVTEVD
                     LRYDNRVIFRERG"
     gene            complement(24068..25045)
                     /locus_tag="VLD60_02780"
     CDS             complement(24068..25045)
                     /locus_tag="VLD60_02780"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSB43905.1"
                     /translation="MGDRPLLTGARIGVLMGGQSSERDVSLRTGAAVHRALCRRGYDA
                     VTIDVGPMLSRNLQDQNIAIAFLALHGPGGEDGAMQGFLETLGIPYTGSGIQASAVGM
                     HKVTTKTILAVHGIPVPPGTVIKRGEKISSAKVLRAAKLRWPVVVKPASEGSTIGVTI
                     VKKVSQWNDALALAHKYDRDAMVEAYVPGHEVTVSLLGRQGEAPLVLPAVEIVAPGGF
                     YDFAAKYEKGKTQYLCPAPLSATVTKQIRTFALKTYEVLGCAGAARVDFRITPRGRPY
                     VLEINTVPGMTETSLLPMAAKQAGIGYEELTERILESAVARMKGSQSAA"
     gene            complement(25052..25999)
                     /gene="murB"
                     /locus_tag="VLD60_02785"
     CDS             complement(25052..25999)
                     /gene="murB"
                     /locus_tag="VLD60_02785"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSB43906.1"
                     /translation="MKGTGRRAKETRAGTRFQKKDLQAAVVGLRGSVTFDASLNVYTS
                     FNIGGPADVLVEPADVEDVCRLVQQANARKIPLFVVGGTNLLIRDGGIRGIVVSLSKL
                     RAIKEEPGSVLYAEGGMGMPTLIGHAIRRSLGGLEWAAGIPGTVGGCLVMNAGTRLGE
                     MKDSVKAVRLVTMKGEILDLPATSIPFEYRRARLPKGIVVGVWLQLRQGVRAELERVV
                     KDYLHYRRDTQPLTMPSAGCVFKNPPKDSAGRLIEATGLKGLRVGDAEVSPKHGNFIV
                     NRGHARAADVIELIGKVRRAIRRKTGLRLDLELKIVGEA"
     gene            complement(26004..27503)
                     /gene="murC"
                     /locus_tag="VLD60_02790"
     CDS             complement(26004..27503)
                     /gene="murC"
                     /locus_tag="VLD60_02790"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSB43907.1"
                     /translation="MTSISLLERQESNVVGQRSNSGLDPSQPVRGLRRIPMFRKTQQI
                     HLVGIGGSGMSGIAEVLLTLGYKVTGSDLQASDTTRRLEELGGRIFIGHQESNVGEAQ
                     VVVISSAVSPQNPEVLVAKAKQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSMVA
                     NVLAQGGLDPTMVIGGKVNALGSHARLGRGELLVAEADESDGSFLRLSPTIVAVTNLD
                     REHLDHYGSMERINESFLEFINKVPFYGLAVLCADDDRLRALFPKIVKRYHTYGLHER
                     EGMTPDFRATDISVKQWGAEFRAFFRGRNLGPFRLSVPGIHNVSNALVAIAIGIELDV
                     PVDLIRKALAAFNGVERRFHLRGEASGIMVVDDYGHHPTEIKATLVAAKQGWPDRRLV
                     VLFQPHRYTRTRDLLDDFAQAFKDSDQLFLTEIYAASEQPIPGVSGAKLADAIRAAGH
                     PSVTFVERKDQLIDQVLPHLQPGDFVLTLGAGDIWKSGTGLLAKLTPAT"
     gene            complement(27466..28593)
                     /gene="murG"
                     /locus_tag="VLD60_02795"
     CDS             complement(27466..28593)
                     /gene="murG"
                     /locus_tag="VLD60_02795"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSB43908.1"
                     /translation="MTIVIAAGGTGGHLYPAVALAREFLRRDPSTKVLFVGTSRGLEA
                     KVLAHEGFELALIDAKPVMGTGLLGTVKGLCAMPLSLWQCRRILRARQVRLVIGVGGY
                     TSPMMVMAAALAGVARVILEPNANPGLANKAVGPYVQRVFLAFESAAASFDRAKVRVV
                     GTPIRREFLERAKQGHAPSREPDRRHLLIFGGSQGAKAINSAAIEGLPELIRERPQMT
                     VTHQTGESDHARVAEAYRRAGVSVTATPFLYDMPSAIQAADLVVARAGAMTVAELTTC
                     GKPAVLIPLPTAIYDHQTKNARVMEAAGAAVLLPQSELTGPKLAQTITAILDNPERMR
                     AMGAASATLRRTDAAESIVRECCALIGDHHDINQPLGATGV"
     gene            complement(28593..29786)
                     /gene="ftsW"
                     /locus_tag="VLD60_02800"
     CDS             complement(28593..29786)
                     /gene="ftsW"
                     /locus_tag="VLD60_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSB43909.1"
                     /translation="MASRSAGTLSLPWPTAGQRPGKQRVAMDHTLLFVTLTLALVGLV
                     MVFSASAIVAGNRFQDPEFFLKRQIAWLGFGFLLMHLTSRIDYTLWKKLSIPILGCMV
                     ILLVMVLVPSLGVAAKGARRWLRLGPISIQPAEMVKLVTVIYLAAYLTKKSDKVTAFR
                     SGLLPVLIVVGVLSGLVLLEPDLGTVVVMGLVTVGLLFLGGARISHLFALGLCAIPAV
                     LVLVLGSSYRRQRLMTFLAPWKDASDAGFQITQSFLAFGSGGPFGVGLGEGKQKLFFL
                     PEAHTDFVLALVGEELGLAGTATVILLFAVFVWRGFQIAARARMPFGKYLGMGITLLI
                     GVQALVNAAVVTGLLPTKGLTLPFVSYGGSSLVVSCVGVGILLSISRDRHAGGSKGGR
                     GESDH"
     gene            complement(29819..31234)
                     /gene="murD"
                     /locus_tag="VLD60_02805"
     CDS             complement(29819..31234)
                     /gene="murD"
                     /locus_tag="VLD60_02805"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSB43910.1"
                     /translation="MRMVEVDRVDLAGAQVTVLGMARSGVAACHLLQEAGARVTVADR
                     KEAGELTAVLEKIDRSRICVSVGTQYETSLEHADLVVISPGVPYRLEALERVRRRGVK
                     VISELELASRFLSAPILAVTGTNGKSTTVTLIGKMLAEQGKRAFVGGNLGTALSEAAW
                     TTLQATKQGKPNPFDYLVVEVSSFQLETIDQFHPWIATLLNVTVDHQDRYDSIDEYLS
                     AKRRIFENQSAADFALFNLDDARVAGLRHHVRAKRLGFSRTQAIASDMDGGTFLDGDR
                     IVTTVTGSRQEICRKSDIRMLGHHNVENVMAAATYAALWGCPTEVIHHVVSTFPGLEH
                     ALELVRERRGVRFVNDSKGTNVDATLKALEGIDHPIWLIAGGRDKGGDFSRLIETVRQ
                     RVKHVILIGEAAPLLKQAWTGATAMSEAGSLREAVEFAAREATAGDVVLMSPACASFD
                     MFADYQDRGRQFKSAVQALPA"
     gene            complement(31234..32313)
                     /gene="mraY"
                     /locus_tag="VLD60_02810"
     CDS             complement(31234..32313)
                     /gene="mraY"
                     /locus_tag="VLD60_02810"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSB43911.1"
                     /translation="MLYIWLYPFHTEYGFLNVFRYLSFRIIYAAVTAFLIAFVLTPWL
                     IRKLQEIKLGQQIRDDGPKRHQAKSGTPTMGGLLIIFAVFLSTLLWADITKPYVWLVM
                     VATLGFGAIGFADDYLKFIKAQSKGLSPTQKFAAQVGVAFLIAIVLYYFIPGYSTKLN
                     VPFFKNFMPDLGWFYVVFVVLVIVGSSNAVNLTDGLDGLAIGPIMIAALAYTIVAYVA
                     GHRIMSDYLLIPHIEGAGEIAVFTAAILGSSLGFLWFNTYPATVFMGDVGSLPLGAAL
                     GTVAVISKHELLLLMVGGVFVIEAVSVIFQVVSFKSRGKRIFLMAPIHHHFEMKGWEE
                     PKVVVRLWIIAILLALLSLSTLKLR"
     gene            complement(32346..33845)
                     /gene="murF"
                     /locus_tag="VLD60_02815"
     CDS             complement(32346..33845)
                     /gene="murF"
                     /locus_tag="VLD60_02815"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSB43912.1"
                     /translation="MTLHSGSRAEQSEEPMALFTVEELREVISVKVLAGDPSPSAKRR
                     IRGISTDSRSIRRGDLFVALSGERFDGHEFVPAVLAKGAVGAIVHDEYRLPHGKGPGA
                     KTAGRTGPFLFGVRDPLFAYQQLATHHRSRFEIPVVAVTGSNGKTTTKEMVASVLAQR
                     WRTLKTEGNFNNRIGVPHTLFRLTARHQAAVIEMGVDQQGQTTRLSEIVRPTIGIITN
                     IGPDHLEFFGSMEGSAQAKAELLDLLPANGTAILNADDPYFDYLAARAQCRVVSFGLS
                     EKADVRATNLSSDPRKGTTFRLLLPGKSRHTVVTIKVHGLHNVTNALAAAAVGFALNL
                     SGATIAQGLARFRPAAMRSQVVTHHGVQIINDCYNANPASMKAALQLLAQWSPARERV
                     AVLGDMLELGRDTQHMHREVGQFLAGLHLSRLIVCGPLGHEIAEGANRAGMSESAIIE
                     TPDAAGAAELLRRMVRQGDVVLVKASRGMKMEQIVQVLTGMRAVTKQAS"
     gene            complement(33842..35359)
                     /locus_tag="VLD60_02820"
     CDS             complement(33842..35359)
                     /locus_tag="VLD60_02820"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSB43913.1"
                     /translation="MERGSMTVGQLVAALKGQVEIVEQHGGLDRAVTAVTDDSRAVAS
                     GSLFVAVKGERADGHTFVPQVVQAGAAAIVAQEAVETGSIPFVRVADSRKALGLIGSR
                     FYGDPSARLAMIGVTGTNGKTTTTYLCKAFLEGIGRRVGLIGTVAYQIGSETIPASHT
                     TPGALELQGLLAKMTQAGLNSVVMEVSSHALAMDRTAGCEYDAAVFTNLTQDHLDYHR
                     TMEEYFQAKLRLFAGLGGGKKAGQRAIVNMDDPYGSQVRAACRVPVWGYAVTNRADLK
                     AEQVRLSMNGTTFTAATPAGTFSVESRLVGEHNVYNLLGAIGVALHAGATCDQVREAV
                     VQVSNVPGRFERVSAGQDFTVVVDYAHTEDALLRLLTAAKALKTNRIITLFGCGGDRD
                     RGKRPKMGRVAVEHSDVVVLTSDNPRTEDPMAILQEVEAGVREALQGRTHVEYHLVPD
                     RREAIGTAVRLARTGDIVLIAGKGHEDYQIIGTKKFHFDDREVAREAIQQLRNCA"
     gene            complement(35368..>35595)
                     /locus_tag="VLD60_02825"
     CDS             complement(35368..>35595)
                     /locus_tag="VLD60_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSB43914.1"
                     /translation="RTGTYSNTQFVGSFVGFLPADSPRLAMIVVIDEPQGEAWGGTVA
                     APVFRRVGEQVLTYLGVSRDDQVKIAMVARE"
BASE COUNT         7400 a        11402 c         9461 g         7332 t
ORIGIN      
        1 ctcggaaact agaaagcgca ccgcgttggc gatgtcggcc ggcgttccta accggcccaa
       61 tggaatttgt ttctgcaggg catctttgac ttctgccgac aacccctgcg tcatggcggt
      121 atcgatgaac cccggcgcca ctgcgttgac ggtcacgttg cggctcgcat attcgcgcgc
      181 caccgttttg gtgaatccaa ttaccgccgc cttcgatgcc gcgtagttgg cctgtccgac
      241 atttcccatg gcgccgacga ttgaggcaat gttgaagatg cgaccataac gctgtttggt
      301 catcggctgc aagaccgcct tcgtacaatg aaaggtcccg ttcaaattca cttgcagaac
      361 gagattccaa tcttcttcct tcatgcgcaa cagcagtccg tcgcgggtga tgcccgcgtt
      421 gttcaccaag atatcgatct tgccccactc tttgatgacc tgctccgcca tcgccttcgt
      481 gtcgttggcg tccgcgacat tcactttgac attcagggcc ttgcgcccaa actgttccac
      541 tgcggccacc gtctccttcg atcgagtggg atcgagatcc gccacgacga tatccgcccc
      601 ggcctgcgcc aacgactcgg caatggcgcg cccgattccc tgtgccgcac ccgtcacgat
      661 cgccacttta ccctgtaatg acattcgctc gctccttaca catcctgcgc gcctaacgcg
      721 gcgcgcgtcg catccagcga cttgggatcg ttgacattca tagtcaccgc atccggtacg
      781 attcttctga tcaaaccggt caaaaccgta cccggcccca cctcgataaa ggtcttcaca
      841 cccatcgctg ccatggcctt gatggaatcc tcccacagca cggaagaggg aagctggcgc
      901 acgagcgaag gctgaatctc ttcagccttc gtaatcgtcc gcgcttcggc gttgttgatc
      961 aagggcatct gcaggtcaga ccatcgaatg gcagccaggt cctcagccag tcgatcggcc
     1021 gcctgttgca tcagcggcgt atggaccggg acgctcacgg gcagcgggac ggccttcttg
     1081 caccccttcg tcctggcaat ctcgatggcg cgctccaccg ccgctttctc tccggcaatc
     1141 actacctggc cgggagaatt gaagttggcg gcctgcacta ccccgaccga gcccgcctcg
     1201 cggcaaacat ccttcacggc atcggccgat aagcccaaga gggccgcgac caatccggtt
     1261 cctggagcga cagcttctga catgtaacgt ccgcgttttt gcacgatgcc cacagcatcg
     1321 cgaaagccca tgccgccggc cgcgaccagc gcggaatatt cgccgagact atggcctgcg
     1381 actgcaatcg ccttgacccc ggccggttcc aaggcacgaa gcgcggccat gctgctcacc
     1441 agcaaagcag gctgcgtatg ttccgtaaga ttgagcttct ccgccggccc ttcaaaacag
     1501 agttgggcga catcgtagcc gagaaccgat gcagcctctt cgtagacagt cttgagatgc
     1561 ggatgggctc cgcacagcgc cttccccatc cctacggact gcgatccttg gccgggaaat
     1621 accaggccga ttcctgaatt cataaggggt tagatatctt agaaatgctc ttgaaagtca
     1681 acgagaaaag ttctttgaag ccttcactgt tcgccctcgg ctctgaccac ttcgaaccgg
     1741 ctcatcgcgc gaaagcgtca gccgtgccta ttaccatcga atcaacgcag acgcccaggt
     1801 caatccagct ccaaacgccc ccagcatgac gagcgaccct tccctgatgc gtccctgccg
     1861 gatcgcctca tcgagggcaa tgggaatcga cgccgcggag gtattgccgt agcgatcaag
     1921 attcaacatt actttctcga gcggcaatcc caaacgatcc gcgacagcct tgaggatgcg
     1981 caagtttgct tgatgcggga catagaggtc gaggtcttcg atcccgacgt tgtttgccgc
     2041 gagtgtttca cgggcaatct cttccagggt gcgcaccgcc accttgaatg tctcattgcc
     2101 cttcatcttg atacagttca atttttccgc gaccaccttt tccgaaggcg gcagtctgga
     2161 tccgccgccc ggcacagcaa tcaactccca aagcgcacca tcggaccgca gatgtgtcga
     2221 cagtatcccg cggttccctt cgctcgcgct gacgacggcc gctccggctc catcgccaaa
     2281 caacacgcag gtgttccgat cggtccaatc cgtgatcgcc gacatcactt ccgagccgat
     2341 aaccaacaca tggcgcatgc cggttcggac ataggcatcc gcaaccgaca gggcatagac
     2401 gaacccgcaa caggctgcag acagatcgca ggcggctgcg cgagtcgcgc ccagttgatg
     2461 ttggatcaaa caagccgtgg ctggaagggg ataatcgccg gtgcaggtgg cgagcagaat
     2521 catgtcgaga tcggctgcac cgatgttcgc tgccgccaag gctcgctgtg cagccttgag
     2581 cgccaggtcg gagcaggctt ctcctgcgcc ggccactcga cgttctcgaa tcccggtgcg
     2641 ctcgacgatc cactcgtctg aggttgccac catcttttcg aggtcggcgt tggccaacac
     2701 cttcgccgga gcgtaggatc cggtccctgt gatgcgcgcc ctcacgcggg cggctcctcg
     2761 gcgggccggc tttgcccgag attttcttgg atatcgcgct gaatcagctc atcgactcgg
     2821 ctctcggcca gtcctttggc tcggcggatg gcattcttga ttgccttggc agacgaacgc
     2881 ccgtgacaaa tcatcgtgat tccgttcacc ccaagcagcg gcgcgccgcc aaattccgca
     2941 tagtcgatct ttcgcttgag attcagtagc ggggacgcaa tcagggggta cgcgagccgc
     3001 ccgaagaacg aaccggaaat ctctttgatc aacagtttct tgatggtctc ggcaactcct
     3061 tcggagatct tcaacgccac atttccgata aacccgtcgc agaccaccac gtcggcctcg
     3121 ccgctataga catcccggcc ttcgacatta ccgataaagt tcaacggagt acttttcagc
     3181 agcttgaacg cctctttcgt gacctcgttc cccttgctgt cctcttcgcc gatactcaaa
     3241 agccccacgc gaggattcac ttttcggaaa agatgtttcc catactcatt gcccataatg
     3301 gcgaactgcg ccagatgctg ggctgtacag tccacattgg cgcccacatc cagcatgatc
     3361 gcctcacccg tcagcgtcgg caagctcgtg gcgatcgcgg gccgttcaac gcctttggtc
     3421 agtcccagca caaagaacga ggccaccatg ctcgcgcctg tattgccggg actcaccacc
     3481 gcatgcgcct ctccgctctt caccaattca gtcgcgatcc aaatggaaga gtcgcgtttc
     3541 ttgcgggcaa ccgcagcagg cgactcgtgc atctccacca cctgcggagc atgtttgatc
     3601 gagaggcgct cgtccgtgca accgagacgg cggcattcat ccgaaaggag cttctcatcc
     3661 ccgaccaaaa tgacgtcgac gtccagttcc ttggccgcct gaagggcgcc ctcaatacaa
     3721 ggaccggggc cgtggtcgcc ccccatggca tcgagcgcaa tctttgtccg cgatgatgct
     3781 gtcacgagta cttcgacact ccttgaatga accagcggca gagtcccact gacgggctcg
     3841 aatacggggg aagtttagag gagtacgggc aacgacgcaa cagattggtg aaagaggctc
     3901 ggccccattt cgacagcccg gcgcgaatgt cgggctgtcg gatgtatcaa caggaccggc
     3961 tagacttctt cgacctgaat gacggctttg cctttatagg tgccgcagtt cagacaggta
     4021 taatggggaa gtttcaactc gtggcactgc ggacagaccg acataccagg cggcgtcacc
     4081 cgcaatttct gagtgcgacg cttatcgcgt ctcgcccgtg aatgtttatg tttcggattt
     4141 ggcatggctc ttcctccaaa cagccggcta ccggtgagct ctgcggctca agccggccta
     4201 ccgatttagt gctttgtggt cctgatcacc cgaatcgccg tcgccggcgg ctctgcgggg
     4261 cactgacagg gccccgcatt caaatcttcc ccgcatcgcg cgcacagccc cgcacaatca
     4321 acccggcaga gcggctgcat cggcgccgcc aggatgatgt gttcacgcaa catgggagcc
     4381 agttcgacat gatcgccttg ataaaaatac cgatcgtcgc cttcagcttc ctcctcgacg
     4441 tccggttcca tcttgaccgg cgccggtcgt cccctgcgtg gctcgacagc cttcggctga
     4501 cgtccgctgc cttttgactc cttgccttct cgagcgaacg ccgcatgcac cgagaacgct
     4561 aatgcatcgt catattcttt gaggcagcgg acacattgcc gaaccacggt gccttccaac
     4621 acgccggtga ccgcaatcat cccgtcggcg ttcatgaggt ccaggctgac agagagcggc
     4681 ccgcgaagcg tcgcatccgc gtcagtcagg ctgagttcct cggccgtcac ctcgcccacc
     4741 aatgaaagcc cctcggctgt gatgtccgcg accagcggtg tgagcagctc catcgtggca
     4801 cctacctgca tatcgatttc caaagccgtt tcttaccgct cttcggcaaa actgatcagc
     4861 gcgatatgac gggcccgctt cactgcggtc gtcagctcac gctgatgccc caagcaattg
     4921 ccggaaatac ggcgagggac aatccgtccc cgttccgtca gaaaattccg caggagcccg
     4981 acatctttga aatcgatgga acccttctcg atgcaaaacc ggcaaggccg gcggcgctgg
     5041 aaaaaccgtc cgccgccgcc agacccaccg ctctctcctc gtccaccgcc accatgctca
     5101 cgttccatga gatcacctct tcaatcaaaa aattactgtg gttcttcgta atcgtaatgg
     5161 ggttcatcat gcgccggggc ctcgctccct ccaccgccgc ccccacctgc atctccccgc
     5221 ttgggaagaa acgtcaccgt ctgcgcaacg acttcgtgtt tgctgcgctt ctggccatct
     5281 tccgtttccc accgccgctg ctgcaagcgg ccatcgacga tgatgccgtt tcccttgctg
     5341 agatactgtc cgcaatgttc cgcctgtttg ccgaaaacga ccacatcgac gaaacagact
     5401 tcttccttca actcatctcc ctgcttatac cgccgactga ccgccagccc catcgaggcg
     5461 accggcgtcc cgttcggcgt ataccgcaat tcagggttgc gcgtgaggtt cccgatcaga
     5521 atgactttgt taaacccggc caccagcaaa ctcctcagac ggcgtctcag ccacgcgccg
     5581 cggcaccagt tccttctcaa ggtggacggt gaggaacttg atgatgttgt cctccaaccg
     5641 gtaggcatgc tccagttcgc caaccgtgtt gttcggcgcc ttgaagtaga agtacacgta
     5701 cgtgcccttc cgctcccgcc gcacttcgta ggcgagcttt ttgcggcccc agttttcggc
     5761 cttgatgaac tgcgcgccgg tctttccggc gacacccttc atcttttcga tgagcgcgtt
     5821 cgtctcctca tcggtaatgg acggacgaat aataaacaga gactcgtaga gctccatgta
     5881 gggatcctcc tggacaaagg cccccggacc agccgggagc gaggtgtagg gcgcctattt
     5941 ggcgcaaaga acggtggaag ttatcacagc cgaggcaaag ctgtcaattt cctgagtttt
     6001 cgacaccgga taggccggcc cgagcgttga gcaggcagcc tccacccttc cgaatgttgg
     6061 ttggaaggca ggctaaacgt tgaacctaaa atacacaata tccgcttctt tgatgatgta
     6121 gtccttgcct tcgagccgga acaacccctt ctccttcacc ttcgcctccg atccgcaagc
     6181 caggagatcg tcatagtgat acacctccgc gcgaatgaag ccccgttcca tgtcggagtg
     6241 gattttcccc gcggcttgcg gcgccttggt gtttttcacg atcggccacg cgcgggattc
     6301 gatttctccg gcggtaaaga aggtaatgat gttgagaagg gtataggcct cgcgcgtaag
     6361 ccggacgagc cccgattcgg tcagtcccat ctccttgagg aagtctgacc gctcgctctc
     6421 cggcagcgac gacaattccg cttcgagctg cccgcaaatc gtgacgacgc gcgccccccg
     6481 tttctcggca aagtcgcgca cggtcttgac catggcctca tcggcattct ttccctctga
     6541 cacattggcg acaaagagaa cgggcttggc tgataggagc tggcattcat caagcaacgc
     6601 ccgttcttct ggctgatagg tcaggttgcc gagccattca cccttgtcga gcaagccgat
     6661 cagtctggtg agaaactcga cctcaaaagc ggctttcttg tcgccggccc gcactttctt
     6721 ttccgtcttc tgcctgcgac ggtcgagcgc ttctaggtcg gcaagcatta actcggtctc
     6781 gatcacgccg atgtcgcgca gcggatttat cccgccgctg acgtgcacca catccgttcc
     6841 ctggaaacag cgcacgacat ggagcaacgc gtcgacttcg cgaatgtgtc ccagaaactg
     6901 attaccgagt ccttcgccct tgctggctcc ttcgacaaga ccggcgatat cccggacttc
     6961 gagcgtgctg taggttgtct tcttcgattt gaaaatatcc gtgagcgtga gcagccgcgg
     7021 atcgggcacg agggcgatgc cggtgttcgg atcgacggtg gcgaacggat aattcgccgc
     7081 cagcgcgccg ccgctggtga gcgcattgaa tacggtcgtc ttcccgacat tcggcaaccc
     7141 gatcatcccg caacacaatc ccatgccggt tatccttctt tctcttccgt agactggtct
     7201 cgcacgttga actgattcat cgccgtttca gttcctcgat gaatcacaca ttcgagcgca
     7261 tcgacggccc gctccaggca cggttcgaca atgtccattt cttctctagt gatgggttcc
     7321 agtacataat ccgcagaatc ctgccgcgga gcgggacgcc caatcccaat tttgatccgc
     7381 acgaactgtg gggtgccaag cgtctcgaca acggacttga tgccgttgtg tcccccatgc
     7441 cccccggcaa gcttgatccg gaggcgtccc ggttcgaggt cgagatcgtc gtggactacg
     7501 ataaggtcgt cggcggtgag cttcaattcg cggagcaggc ctttgagcgg gggacccgtg
     7561 acattcatga agtcgagggt gccggccagt tcgatcaatt cggaaccgag ccgccctgac
     7621 ccacgctgag cgatgccgcg cctggaaaga tggatcgacc atcgagctgc cgcccgctcg
     7681 atgacccaca tcccgacgtt gtgacgggtc tgggcatagg aggcgccagg gttgcccagt
     7741 ccaacgatga ggcgcaacgt tacttcttct tttcggcttc tttcttttca gccttgggag
     7801 ccgcagccgc ttcctttttc tctccggctt tggcttcgcc agccggcgcc gcgggtccag
     7861 gcgctgcggc cacaccttcc gccccttcca ctgcagcctt gcccttcgca accacttcgg
     7921 gctctttcgc cccttccggt cccacagcgg cgctggtcag caaggcttcg agcttggcct
     7981 ccgacatcgg caccgcgacg ctcacaacca tctgctccga atcgtcgaga taacggagcc
     8041 cttccttctt gacgatttcc ttcaaatgga taccctgccc gatcgcgagc ggtgaagcgt
     8101 cgacttcaat atagtccggc aatgcggcag gcaagcactc gacgtgcagc tcgcgcatat
     8161 tgtgatggag aatgccgccc tccttcacac caaccggggt acctccaatc acgtgcaccg
     8221 gcactttgac tcgaatcggc ttttccatcg agatctcgaa caggtcggcg tggagcactt
     8281 gcccgtcgac gggatcgacc tggtaatcac gcaggagggc ggtccgtttg ggtttggact
     8341 ttgcccccgt gatggtaagt gagatcaagg cggtgctgcc cgcctgcgac ttgagaatct
     8401 tgaccaacgg ctccggatcg atggtcagta agaggcactc gccctgtccg taaatcacgc
     8461 cggggacctt cccattgcgc cggagttggc gtgcggcacc cttccctgac ttttccctca
     8521 cgttcaccgc taaatcgaat ttcatggtct tcctccgtcc ccttcttctc gttccagatg
     8581 cctcggtccc gctttcaggc aaacagggaa ctcaccgact catcctcgtg aatacgtcgg
     8641 atcgcttcac ccagcagcgg agcaaccgac agttgatgga tctttgggca aatcttttcc
     8701 ttgccccgta atggaattgt gttggtggca atcacttggg tcagacaaga attctggagg
     8761 cgttctaatg ccggcccgga cagcaccgca tgggtgcagg cggtccacac ttcccgtgcg
     8821 cccttgtcgg cgcaagcctg cgcgccttga acgatcgtgc cggcggtgtc gatcatgtcg
     8881 tcgagcagga gcgcgctctt cccccgaata tcgccgatga tattcatgat ctgggtctga
     8941 ttcggcccct cccggcgttt gtcgatgatc gcaagactgg cttgaagccg tttggcgaac
     9001 gcgcgcgcac gctccacgcc gccggcgtcg ggcgacacaa tcaccaaatc gctgattttc
     9061 tttttcgtaa tgtagtccaa cagcaccggc aatgcgtaca gatggtcgac cggcacgttg
     9121 aagaaacctt ggatctgccc ggcatggaga tccatcgtca ggactcgatc ggttccggcc
     9181 gtgctgatca gatcggctac aagtttcgcg gaaatcggga cgcgcggctg gtctttgcgg
     9241 tcttgtcggg cgtacccgaa ataggggatg acggccgtaa ttcgattggc ggaggaccgc
     9301 ttgagggcgt cgatcatgat cagcaactcc atcaaagagt cgttgaccgg ctcgcacgaa
     9361 gactggacga cgaacacgtc cgccccgcgc acgttttcct caatcttgac tcggatttct
     9421 ccgtcgctga acgacgacac cgtcgcctca ccgagcttga cgccgagata ggcgcaaatc
     9481 tcgtgggcga gggccggatt cgcgttgcca gagaaaatca tcaattctct gctcatcgat
     9541 ccctcgaacg gtcctcagat attatgtggt gccggtcctg ctctcgtacg tggttcgtct
     9601 cgacggcggg cgtcggtggg cgtgaaaggt acccgcctac ctcgttcgct acgcgctgtt
     9661 gaggaagcca actaagggtc tgaactgtaa cggattgcct gttcgcttgt caaccgagcg
     9721 atgctttatc ccagtctccg ctaccgaacg gtcaacggcc ccgaacaggt cggaacgacg
     9781 aagactttga gccgtttctc ctgtgcaaat tgggcctcag ccttatgggc cgtgccttcg
     9841 tcatgaaaca acccgaacac ggtggctcca cttccggaaa ggagtgccag ctcagccccc
     9901 tgggcaagca gggtctgctt gatacgccga agcgattcat gacgggcaaa caccggcgcc
     9961 tcgaagtcgt tttccgcagc cgcgacgact tgagcccagg tcgcacaagc ctgccgatca
    10021 aggtcctcat gcatcgacga caatggacgc actgcctccc tggtcttcgc caattcttga
    10081 taggcccatt tggtttcgac gccaaatccc gggttgacca ataccaccca gcggcgctcc
    10141 ccgatcgcga tcggcttcac tgcttctcca cgcccagcga ccacgcccga aggagagaag
    10201 aggaagaacg gaacatcact ccctagcagc tggccgactt cagccatctg tgcgatggac
    10261 caaccgaggt gaaagagacg attcaatccc aggatggtgg ccgcggcatc actgctccct
    10321 ccgccaagac cggcccccat tggaatccgc ttcagcagtt gaatgtccag tcctgtcgct
    10381 tgcggtgcgc gctccagcac ggcagccgcc gctcggtata caagattgct ttgacctgcg
    10441 gccagttgtt ccgtatcaca acgaagttga atgttatttc gatgaggcgc aagccggaac
    10501 tgcagttcat cttccagcgc aaccgtttgc atgatcgacc aaagataatg atagccatcg
    10561 gaacgacgat cgagaatgcg caagaccagg ttgattttgg cgggagcgga aacggtaaca
    10621 acggagagtg gagaagtggt cgagtctgag tcgctcttag tcacggcctc cttttatagc
    10681 atacttttcc agccggtagc ggaaggagcg ggtgttgagt ttcaagagac gcgccgcttt
    10741 cttcttcacc cacttcgacc gttccaaggc cttcaccagc aagtcctttt caacgccgtt
    10801 gataagggct tctagatcca gtccgtcttc gggaagatca gtcggaaccc cctgcgatag
    10861 ttgcggtgtc accgcgggat gcagccagcc tctgatctcc gcgtcagtca caggagctcc
    10921 ggttgaaaaa gctacgaccc gttcgatcag gttctccagt tcgcgcacgt tgcctcgcca
    10981 ttcatgctgc aagaggacgt gcatggcctc ctggtccaac accggcgccg ccttcccgct
    11041 ttcttgagcg aacttttcca ggaaatgttt gaccaacagc ggaatatctc ctgttcgcag
    11101 ccgaagggga ggtagacgga tcgggatgac gtccaatcga taatagagat cttcccggaa
    11161 cgacccatcg gcgacggctt tctcaagatc gcggttcgtg gcggcaatga cccgaacatc
    11221 gaccttcatg tcctgattgc cgccgacgcg ccggaattcc cgctcttgaa tcacgcgaag
    11281 cagcttgact tgaatcgtcg gcgtcgtgtc cccaatttcg tccagaaaga tcgtcccccc
    11341 gttcgcaact tcaaacaagc cggccttgtt cgcaatggcc ccggtgaatg atcccttcat
    11401 gtggccgaac aattcgctct cgagcagagt ttccgggact gcgctgcagt tgactgccac
    11461 gaacggcatc gtactgcgtc cgctgttgta atggatcgcc cgcgcaacaa gttctttgcc
    11521 cgttccgctt tcgccacaaa tcagcacatt gcttttcgca tcggcaacct tgcgaaccac
    11581 atcgaatacc ttctgcatgg cttcactttg cccgacgatc cgcgcgaacg acgattggct
    11641 cgccatctcg cgcttgagca ggatgttctc tgtggaaagc cgtcgcttct caagcgcatt
    11701 tctgatgatc agctgtactt cgtcaacctg gaagggcttt gttaaataat cataggcccc
    11761 ctgcttcatc gcctccacag ccgaatcggc ggtcgcaaac gccgtgacga ccaggacgac
    11821 ggtctcgggg gatgacgact tcacggcctt gagcacctcc atgccgtccg ctttcggcat
    11881 gcggagatcc gtaatcacga ggtcgtaaat ctccttgttg acctgaccga tcgcttcctc
    11941 tccatcggaa gcttcggtga cggcgtatcc cgcccgcttg agcatgatgc tcatcacctc
    12001 acggagactc ttttcgtcat caacaactaa gatcttttcc acggtgttcg tccctcatgc
    12061 cacaaccgca ctccgttatc cccggaacgc ggcatacaaa ccacaaaacg agttccctgc
    12121 ttcaaatgac tctcgacttt gatccacccg ccgtgcagat cgacgatgcg atggaccgca
    12181 gccaggccca accctgatcc ttctttcttt gtcgtaaaga atggaaggaa aatcttatcc
    12241 aggttgtctt tggcgatacc gtctcccgta tcctgaaagg caatttcaac gacgtccccg
    12301 tgacgcccac cggcatcgac gcggcggcac ccggtggcaa tcgttaactg tccgccggcc
    12361 ggcattgcat cgaacgcatt cgtcgcgaga ttccagaaca cttgtcgcag ctgatcctga
    12421 tcgacctgcg cgagaagagg gcggccagtc agcgacgtca cgatcgtgat gttcttccgc
    12481 gttcgagctt catgttgtac cagatcgaga gtctcggcaa gaattttgtt catatcgcac
    12541 tcggtaagag tgagcgcggg cggccgtgcg tactgcaaga attccgtaat aatggcgtcc
    12601 aacctcgtgg cctctctgat agcgatgtcc atcaggcgac ggtctgtctc atccgccgac
    12661 aattctttcc tcaacatctg catggcgccg gctaacgcgc ctaatgggtt tcggatttca
    12721 tgcgccattc cagccgacat ttccccgaga ttggccagcc attcctttcg gcgcatctct
    12781 tcttccatat ccctgatctg tgtcagatcc ttgaacactc cgacgagtcc cccctcctcc
    12841 ccttgctcat gcaaaggaga aaccgtcatg ccgagcacca gccgattgcc tcttgagctc
    12901 ttgcactcca cttcaaatcg cgtcatggaa ggcggaacat cttcctgctg gtcgtcggaa
    12961 gcgtggttag gatgccagtt aaaaagctct cgccaggaac gaccgatgac ttgacccagc
    13021 gcatagccca tgatttcttg cgcagcagga ttaaacgagg taattgcccc gcgtgcatca
    13081 gccgtgaaga caccgctgct gatactttgt acgatgtttt catggaaggc ctggagccga
    13141 tagagcccct gttccttctc ccgtaacgac tggtcggcct gctgtaattg atccgccaaa
    13201 gttccgctca gcagcccgac caccagaaga gccagggcat acgatccaaa ggtttgaaag
    13261 atttcaggcg ccgtcaggtg actttgctga agccatcccc aaccgtcagc taaaccatac
    13321 agctgaatat tcgtcacgag accaaacaag ataatgcccc cacaggcggc aagaagcccg
    13381 acacgccgct gtggaataag gctggcgaca gcgactgtta tgatgtacag aacggtaaac
    13441 ggactttcga cgcctccggt tcgtgcgacg agcacagttt ccagaaagaa atcgatgccg
    13501 agctgtatcc agaaaaaggc ggctagggtc gctgcggtgt taagccggcg aaggacgagc
    13561 gcggaaggga tggtgagggc ataggtgaga atgatgagcg catagaacgt ctcgactcgc
    13621 tcgccgtttg tcgcctggaa gattagcgac agcccaagca tcagggtgac aacaactacc
    13681 cgaagcccca ttaaccaatg aagcctggtc cgcaactcat ccatagcaaa tgtcaactag
    13741 ttcaggcgac aactgcggag attcttcccg aagcggggtg tggaagtgtg gtcctctcat
    13801 aacatggaag gctcaccatc ccgtcgactt caagcactct gcgacagaga gacggcaaat
    13861 tcatcgggat cgtttgattc ttccatacaa cacaaacgcg actatctatt ctatctgcac
    13921 gtcaaatccg acctcattcg cgcattatcc gattgcagat gccatcgtga aaattggcaa
    13981 atacatcgca atcacgataa aaccgatgat cacgccgaga atcaccatca taatgggctc
    14041 gagcaaggag gtgagcgacg ccaccgcctg atcgacctca tcctcataaa agtcggcgat
    14101 ctttcccagc atcgcgtcca aggcaccggt tgattcaccc acggcaatca tatgcgtaac
    14161 catcttcggg aaaaccccgc tcgttgccaa ggggtcagcg atggtcttcc caccactgat
    14221 gctcacacgt gcattgacta gcgcctcttc gatcactttg ttgcccgagg tcttggcgca
    14281 gatggttaaa ccgtcgagaa gcggaactcc gctcgagagg agcgttccca gagttcgagt
    14341 gaacttcgca accgaagctt ttctgatcag atccccaaag atcggagtct tcaagagaag
    14401 cttgtctatg gccattctgc cctttggtgt cgcataatat tttttgatgg ccatggcaca
    14461 accaactatg atccccaacc cgacgtacca atatgattgg aagatattgc taatatcgat
    14521 aactaactgg gtcggacctg gcaagcccat cttcccgccc gacatttcct tgaacatttt
    14581 ctcgaagaca gggataaccc agatcatcaa caccataatc actattgcgg ccacaccgag
    14641 aatggccgat gggtaaacca tggcggactt gatctgcccc ttcagcttca tcgccttctc
    14701 gatatatttg gcgagacgcg tcaagatggt atcgagcaaa cctcccacct cacctgcatg
    14761 caccaaattg acatagagat cgtcgaagac cttaggatag cgccgtaagg catctgaaaa
    14821 ggtcgagcca gcctcaacct gggtttttac ttcgccaatt gtctcacgca gcaccttgtt
    14881 ttcagactgc gtggagagga tctcgaggca ctgcacaagg ggaaggcctg cattaatcat
    14941 ggtgccgaac tgtctggtaa aaacgacaag gtccttgtcc gtgaggccgg agccaagaga
    15001 gagattgaat ccccccttac ccgatttttc ttccaggctg gtcacgacga cgctttgctt
    15061 gcgaagctga tccaccgcct catctttcgt cttggcggag agttcaccct tcttcacagc
    15121 cccggactta gtgcgtccga catacgcaaa tgtcgccata cgtcactctc acccttcact
    15181 gtggccgatc tacggccggt ttttctcaca gaaatgacta caaagcgagt ctactggcga
    15241 ccctaaaagc tgtcaaacaa atgcacaata tgcgcgcatc cgctcaactg attcgagcga
    15301 cgcaagggag gaagtcagga gttcgaggac ccagtgtgca ggtagcctcg gtagagataa
    15361 tgaaacccgg agcagacggt gaatgccatc atgccgagaa gcagaggggt tagaactgcg
    15421 aggtccatat ggcgggagct gagaaaaatg acgagcagca cgtaggagag ttggagaaag
    15481 gtggtcccct ttccgagaaa cgtcggggcg atattaatgg gggactcgat aaactgcgct
    15541 accgcggtcc ccaacaacaa catggcatcc cgactgacga caacgattgt gacccacgat
    15601 ggaatcaagt ggatcgacga aagcgttatg aatccggaag tcaggaggag cttatccgca
    15661 aggggatcca agaacgcgcc gagccgagtc cgttgattcg cggcgcgcgc gatagtcccg
    15721 tccaacgcat cggtcaatcc cgccacgagc aagacgatca acgcctgatc aaagcgctcg
    15781 tacaccaaca gcccaatgta cacggggatc agcagaatgc ggagaatagt gagactgttg
    15841 gggatattca tgcgagcatg aggagcgtcg gcaccctgga gcgatgatag gagggccctt
    15901 gcagtgatgt caacgtcttg cagtcaacca ggatcctccc ctatactgac gccattcgac
    15961 tgtgagctgg atcgcagtgt ctagggaggt cggcatgagt tccctgtctt ctctgttcaa
    16021 gaaagaaggg ttagttgaaa agcatcaact tgaaggagtt gatcccagcg atcgatactt
    16081 caaccgggcc attctcgtga atcgtacgcc ctctggctat gccgccaagg ttatgtacga
    16141 agcgctgact gtcgaaggcc aatcgcaccc caccatttcc gcagccgtca aagagctcgt
    16201 tgaaaaactt caaccacttg ggttcagccg gctgcgaacc agagcaaact tcaaaggcac
    16261 caaatacctg gctgaaaaag aaacatgggt tgattaccaa gatccagcct aatcacttcg
    16321 ctcctgacga gacgaattcc ttgaatacca ggtcgtgaat ttttggtcgg aacgcgcgaa
    16381 tcatgtcgtt ctttcggcac gggtgtacgc gattcgagag taagacgacc tccagttcct
    16441 gctgcggatc gatccagatg gacgtccctg tatagcccag atgtccgaac gacttcggag
    16501 agaaaaacga gcctgacgat gaaggtgtgc tcggtgtatc ccaccccagc gcccaactcg
    16561 actgcggtat gtcagactgc cgcttggtga atgtccgaac cagctcgccg tcgagaattg
    16621 acgattgctt gcgatgagct cgaagccagg ctcccgtgag gacgagcacg gcttccgccg
    16681 tcccgaacaa gccagcgtga ccggcaactc cgcccatcgc cgcggcattc tcgtcatgta
    16741 cttcaccgtg cagcactctt cctctccatt catcccactc tgtcggtgcg atcttcgaag
    16801 ggtgcactga acgagtcatg gcttgttcgg aggtaccagc cgtcagcgga ccaaacagta
    16861 gaggccacgc cttcagcggc tctgccacgg ccctctgaaa ctgctgatcc aacgtcaaat
    16921 tcgtgactcg ctcgatcgcc agcccgagca gtatgaatcc aaggtcgctg tagaggctcc
    16981 gctctctcct tccatataga aggggttcat cccggatgag ttgcagcacc gcctgccgaa
    17041 tgaccgccgg ctgacccgag cccttctcga gcccctttga ctctagacgc tcatagagcg
    17101 gtcgccagcc ggggagtccc gcactatggg tcaagaggtc tcgtacggtg gccagaccga
    17161 ttggagcccc ctctaattca ctcaagtgct gctggatcgc gttctcaagt cggagccggc
    17221 ctccctgcac caacaatagt accgacgtca ccgtgaccag aggtttggtc agagaagcaa
    17281 gatcgtagac cgtcgatggc tgcaccggct cgcccgaagg gtctgatgac aatcgaccgg
    17341 acacgaccag gcaacggatc gagccgcgca gccgaacggc tagaaccgcg ccgggaaaca
    17401 ccccgtcctg aacggcctgg tccatcgccg tttgaattgc gtccggttct gtcataggaa
    17461 aactttagaa aggatcgcgg gggaacggac aaggagatgc cgcatcgtaa gcccttcaac
    17521 aacttcgacc ggcaaggcag cggccacgcc ttcaaacaac ctataacgcc gggctcgatt
    17581 ccccctcgag tttgtgttcc ggagtcgtgg cgctatcgtg gaaggcctct tgttcttcca
    17641 cttccagcat ccgttcgaat gtacgcagag tcgcgcgcag ccgctcgacc atgagcaagc
    17701 gctgcttttg caatgccgac agatcacgtt gcgtattcgt caattccacg cgcgcctgac
    17761 gcatcatctc accggccttg agctcggctt ccttgatcac cagttccgac tcccgctgtg
    17821 cactgcgctt cacgtcctcg gcaagcgatt gcgcggacac caacgtattc gatagtgtgg
    17881 cttcggttcg cttcaactcg gcgagttgtt gttcaagaaa cacgatcttt tcctgttgta
    17941 cggcgttgtc ccgattcagc aactcgaccg tttccgcgat ttcttcaaga aagcggttaa
    18001 cctcctcctt gtcgtaaccg cgaaagctga ccttgaacac cgtttgttga atatcgagcg
    18061 gtgtaatctt catggtgtcc cccttcaaga attcagttca tctgccgagc gaggtcgctc
    18121 aacgttctga ccagtgcgat ctgcaaaaaa ctaatgataa gaatggcgat gatcggcgag
    18181 agatcgatgc ccatcctcca acccacccaa cgacggatcg gcgccaacac cggttccgtc
    18241 acccgctcca aaaattgcac gatcggattc caggggtccg gattcaccca ggagatcaag
    18301 gcccgggcaa tgatcaccca catatagagc cataagacgg tgtccaaaac cgtggccacc
    18361 ccttgcagca cattgcccat cacgaacatc agcgccccaa ctcctgcgac cgtttcgtgg
    18421 ccgcctccac agcacccatc agtgtagcac ggaacccgcc ctgctcaagc tgatggagcc
    18481 ccgcgattgt cgtgccgcca ggcgaagcca cgcggtcctt gagcttcgcc ggatgctctc
    18541 cggattcgag aaccaatttc gcagcaccga ggaccgtctg cgcggctaac aactctgccg
    18601 tctggcgcgg aagacccatt tttacgcctc cgtccgccaa agcttcaata gcctggaaga
    18661 cataggccgg tccgctgccg ctcagtccag tgactgcatc catcaatcgc tcttcgaccg
    18721 gaacaacgcg ccccaccgcc tcgaagacgg ttcgcgccac gcccttatcg tcttcggaaa
    18781 ttccttcgct gaaggccaac gcggtcatgc cttctcgcac gagcgcagga gtattcggca
    18841 tcgcacgaac gattctcctg gcgccggaca cctgctctcc gatcgagcga atggtcacgc
    18901 ccgctgcaat ggaaattacg agcgtacgac cgagagcggc ccctatttca tgtaaaacat
    18961 ccggcatcgt ttggggcttc accgccagga tcacaacctc cgcccacgac acagcttgac
    19021 gattgtcgga tccgacgcgg ataccgaacc ggctcttcaa tcgatcgcat cgtgcagcca
    19081 ccgggtcggt ggcccagaga gactcggact tgcacagctg gcccgtcagg agccctccga
    19141 tgatggcctc ggccatttgg ccgcccccga tgaaggcaag cttctttgcc atcatattag
    19201 acatggcgtg ctccgaaaat agccgtgccc acccgaacca atgtggctcc ttcttctatc
    19261 gccacctgat aatcattcga catgcccatg gacagttcat tcattgagat ccggggaatt
    19321 gcctgaagtg ccaaccgccg gccgagttca cggagggctc gaaagtacga gcgggcctgt
    19381 tctgccactt ccaccggcgg tggaatggcc atcaagccct ttacggccac gtgcgtcaac
    19441 gttccaaatc gcgacagcgt ctgctccagt ttaccgggct cgaagccggc cttgctcccc
    19501 tctcccccga tgttcacttc gagaaggatc tcttgctgaa gtccggcagc ctcagcgcgc
    19561 cggtcgatct cctgcgcgag atcaaagctg tcgacggaat ggatcagttc aaagatcccg
    19621 atcaccgatc gcgctttccg tcgctggagc tgaccgataa aatgccattt gatcggctcg
    19681 ccctgcaagg cctcgatttt cggaagcgcc tcttgcaggc ggttctcacc ggccaatgtc
    19741 agcccggcag caatcgcctc gcggatcagc tcggacgaaa ccgccttggt cgcggcgaca
    19801 agccggacac tccctttcgg acggccggat gcctgttcag cggaacggat ccggtccaac
    19861 acggagctga cacgttcgcc gattgaccca ctcgtctgtg aatccatgct gattgcgaag
    19921 agactgccat ccgcgcttgt cggcttattg taccgaatcc ggcaggacga cctctaccga
    19981 tgcgccgcca acgtaatccc gctgagcatc gtcccattta cgcgtccctc tcgccgatac
    20041 gagtagaaca ggtcgctatg acaaattgtg cagagattca ccgtcgtcac atgttccgga
    20101 cggatgccaa tttgggtaag ctgtcgccgc actaattcct tgagattcag cctggctttc
    20161 gctccccgat catcctggac gacggcaggc cagtccgacc atccgcttcg cagcggagcc
    20221 agcaccgtct cgtctacttc gtaacaacag gcgcctgctg aaggaccaat gctgactcgc
    20281 aagtcgcctt cagcggattt gaattgcgaa atcaggagat cgatcgtcct gggaacaatg
    20341 ccagctacgg ccccgcgcca gcctgcgtga atggcagcag cggctctgcg gtggcgatcg
    20401 tagaccaata ccggaacgca atcggccgtc cgaacggcta ctgtcacacc tggctgatca
    20461 gtcacgaggg cgtcccagcc accgtcaaac cgatcccctt cggtcagggg tcgatccagc
    20521 accaatgcat cggtgccatg gacttgcttc acggacaaca accagcctcg tccgcgtgag
    20581 cctcctgccg gcactgggac accaacatcg aacgacaccg tctcggcatg gcctcgcgtg
    20641 ccgaaaaaat gttgcacgcc gtcgcgcata gaagcgaacg ccggtactgt aatcaccgat
    20701 gcggccatgg ccaagcacgc tcctcctgct cagtcagcct gcctgcggag aaatgtcggc
    20761 acgtcccatt catcgtcggc aaccgccgtc attcgctcca tggtttcacg tgtttctccc
    20821 atccgccgca ggaacgtagg gcgatcaaga tccttgtgcg gccgctcggc caccgcagcc
    20881 ccgacaccgt tcaacacctg ctgggttgcg cgggccgtcc tggtggctgc taccgcaaca
    20941 ggcatcggtt gttcatcgcg ttcaaacccg gtcgcaatca ccgtcacaac cagatcgtct
    21001 cccatatccg gattgatcac ctgtccgacg ataatgttgg cttcgggatc ggcagtctgc
    21061 tggatgattg aagccgcttc ttcgatttca tgcaacgaca tattcggtcc gcccgtaata
    21121 ttcagcaaca cgccgcgtgc gccctccaca ctaccttcct cgagcagggg gctgcaaatg
    21181 gccttttgcg ccgcttcaat cgcgcggttc gtgccgcgcg agacgcccat ccccatcaca
    21241 gcacggcccg tatgtgacat gacggttctg acatccgcaa agtccacgtt cacatggccg
    21301 gtcgtcgtga tgacatccgc aatgccttga atcgcttggc gcagcacatc atcagccact
    21361 ttgaaggctt cgagcaacgg cgtcgcctta tcaacgatcc cgagcaaacg ctggttcgga
    21421 atgaccaaga gcgtatcgac atgccgccga agatcgcgaa tcccttcgtc ggcgtgagtc
    21481 atccggcgat gtccctcata ggagaacggt ttcgtcacca ccccaacggt caagatgccg
    21541 agttcacggg cgatgcttgc cacgatcggc gccgcaccgg ttccagtccc tccgcccatt
    21601 ccagccgtga caaagaccat gtcggcgcct tcgagacatt cgcggatctg atctttgctt
    21661 tccagcgcgg attctttccc gatttccggt ttggctccgg cgccgagccc cctggtccgt
    21721 tcaggaccga gttgcacttt ataggcggcg cgcgaccgat ccagcgcctg gagatctgta
    21781 ttggcggaga taaaatcgac tcgggctaag cccgaggaga tcatcgtatt gacggcgtta
    21841 caccctgctc ccccgacacc gatgacttta atacgaaccg gcgaaagcac atcttcttgg
    21901 aatgagaaca tcaggacacc tcccttggag aagccgcgcg gggcctggcc gcgcacacag
    21961 gttaaaagaa ctcgaacatc caggatttca tgcgatccaa cactttgccg atcggccgac
    22021 cgcttcggag accggcggta gccatttctt ccgtatgacg ccgcgcatgg agcaacaacc
    22081 cgactccagt cgcatgcatg ggattgctca cgatgtcgcg aaggccgccg accccggccg
    22141 gcgcgcctcg tcgagccggc agattcaaca catgctcggc cgcgtccggc atgccttcga
    22201 gcaacgaggt cccgccggtg accacgacgc cggctcccag catgccttca tagccggccc
    22261 gggtgatctc acgacgaacc aactcgaaca tctcttcgac acgcggttcg agaatctcgg
    22321 cgatgtcgcg acgggaaaac gtcctggccg ggcgatctcc gacagacggc acctccaccg
    22381 tttcatggcc ctgcaccagc tccgttcggg ccaggccgtg ctgcaccttg atcttctcag
    22441 cctcggtttg cgaggtgagc agcccaatgg caagatcctt cgtcaaattc tggccgccga
    22501 tgggcagcac cgcggaatgc cggatgcttc cgtcgagaaa gatggcgagg tcggtcgtcc
    22561 caccgccgag atccaccatc gcgacgccca aatcgcgttc ttcttggctg aggaccgctt
    22621 cgctggaagc caagggctgg agaataatat cgacgacgtc gagtcccgca cgattcacgc
    22681 ttttgatgat gttctgcgcc gacgtcaccg ctccggtaat cacgtggacg ttcacttcca
    22741 agcggttgcc cgagagaccg agcggttccc gcacaccctc ctgtccgtcc accatgaatt
    22801 cccgcggcaa cacgtgaaga attcggcgct cgtgcgggat gaccgcgagc gttctcgcac
    22861 tctcaatggc gcgctgaatg tcatcgcgcg tcacctccgc cttcttgagc gccaccacgc
    22921 ctttgcaatt ctcggccgag atgtggctgc cggcgatgcc ggtatagacg gagttgattt
    22981 gcaccgccgc catgagctcc gcttcctcga ctgccttctt gatcgattcg acggtactct
    23041 cgatatcgac caccacaccc ttgcggaggc cacgggacgg actcgatccc acgccgatga
    23101 tgttgagcgt cccttcctcg gtgacttccg agacgatggc gcatatcttc gtggtgccga
    23161 tgtctaagcc gacgagaatc tgatcgcgtt tcgccacagc ctcaccccct ctccctgaaa
    23221 atgacgcggt tgtcgtaacg caaatccact tcggtcactt cgcgcttcct gccttcaaac
    23281 gtcgtcgccc tggaaactgc tttgaccttg gtaaaccggt cccattgatc catgagcgca
    23341 tcgctcccga attgaaaacg cacgcctttg gccgaggcaa tcaaaccgac gggattccca
    23401 agttcgatct ccacacgacc atcgactgac tgggccatcg ccttggccag ctcgatcgag
    23461 gattggatgg tctgacgcag gtgtcgatcg ccctggagga ggactttggc gtccaacccg
    23521 gtcagcaagg gtaatgtcgc gtcatcccgc gcacccaaac gagcgaggac tgcgccaccg
    23581 tcgtcggtca gaaagtgttc ggatccgatc cgagcgaccg ccgccggctt tcgttccaaa
    23641 actgtcacgc gcaattcatg caacggcaat cgttcgactg tggcctcttt gatccaggga
    23701 tgagtctgca agcgctcggc gagataggac agactgactt ggtggagcgc gatgcccttc
    23761 ttcagcgcca actggttcag aatgtcctga atcgtcactt gatggattcc ctcgacgcgc
    23821 acatttcgaa tctcgagccc ccgctggatc accggaccga gcgcgcgcga gccagccaca
    23881 ccgaccgcaa cgatgatggt cgcacccaga atccagcccg tcaggcgcaa cgcgcggcgg
    23941 catttcactg ctagctgggt tcgacgcacc tgctgcctga gttccgctgc ctgcattgca
    24001 cgcggccctt tccattgatt catgcgatgg ccagctgcct tggtcggccg ttttcggaac
    24061 gggatcttca tgcagcgctc tggctcccct tcatcctggc cacggcagat tcgagaatcc
    24121 gctcggtcag ctcttcgtaa ccaatcccgg cttgctttgc cgccatcggt aacagactcg
    24181 tctctgtcat gccgggcact gtattgatct ccagcacata gggacggccc cgcggcgtga
    24241 tacggaaatc cacgcgcgcc gcgccggcac aacccagcac ttcgtacgtt ttgagcgcaa
    24301 aggtccggat ctgtttggtg acggtggccg agagtggagc cggacagaga tattgggtct
    24361 ttcccttttc atacttggcg gcaaaatcgt aaaagccgcc cggcgccaca atctcaacgg
    24421 ctggcagcac caacggcgcc tcgccttggc ggccaagcag cgagaccgtc acctcatggc
    24481 cgggaacgta ggcctcgacc atcgcatcgc gatcgtactt gtgggccagg gccagcgcgt
    24541 cgttccactg agagaccttc ttgacaatgg tcacgccgat cgtcgatccc tcggacgccg
    24601 gtttcaccac caccggccag cgcagctttg cggcgcgcaa aactttcgca ctcgagatct
    24661 tttctcctcg cttgatgacc gtcccaggcg ggacaggaat gccgtgcacc gcgagaatcg
    24721 ttttggtcgt gaccttatgc atgccgacgg cgctcgcttg aatgccagag ccggtgtagg
    24781 gaatccccag agtttccaga aagccctgca tggcaccgtc ttctcccccg ggcccatgca
    24841 aggccaagaa ggcgatggcg atgttctggt cctgcaagtt ccgactcagc atcggcccga
    24901 catcgattgt caccgcgtca tatcctcgac ggcacaacgc acgatgcacg gccgccccgg
    24961 ttcgcaacga gacgtcgcgt tcggacgatt gcccgcccat aagaaccccg atcctggcgc
    25021 cggtcagtag tggtcgatcc cccatgatgc cttacgcctc accgacaatc ttgagttcga
    25081 gatccaagcg cagacccgtc ttccgacgaa tggcccgccg aactttcccg atcagctcaa
    25141 tcacatcggc agcccgcgca tgccctcgat tcacgatgaa gtttccatgc tttggcgaga
    25201 cttctgcatc gccgactcgc aagcctttca aacccgtcgc ttctatgagc cgtccggcag
    25261 agtctttggg agggttcttg aagacgcaac cggcactggg catcgtgagc ggttgggtat
    25321 ctctccgata atgcagatag tctttcacca ctcgttccag ctcagcccgc acgccttgtc
    25381 gaagttgcag ccataccccc accacgattc ctttcggaag gcgcgcgcgc cgatactcaa
    25441 acggaatgga cgtggcgggg agatccagga tttcaccctt catcgtcaca agccgcacgg
    25501 ctttgaccga atctttcatt tctcccagtc tcgtcccggc attcatgacc agacagcctc
    25561 caaccgtgcc ggggatgccg gcagcccatt cgaggcctcc gagggatcgg cggatggcat
    25621 ggccaattaa ggttggcatc cccattcccc cttcggcata gaggaccgat cccggctctt
    25681 ccttgatcgc gcgaagcttg gagagactga ccacgatgcc ccgaatgccc ccatcacgga
    25741 tgagcaggtt tgtcccgccc accacaaaca gcggaatctt tcttgcgttc gcctgttgaa
    25801 cgagcctgca cacatcttcc acatcggccg gctccaccaa cacatcggcc ggcccaccga
    25861 tgttgaacga ggtatagaca ttcagcgatg catcaaaggt gacggacccc cggagtccca
    25921 ccacagccgc ttgcagatcc ttcttttgaa atctcgttcc cgcacgcgtc tccttcgctc
    25981 gtcgaccggt gcctttcatc gcgtcatgtc gcgggagtca acttcgccaa taagccggtt
    26041 ccggatttcc agatatcccc cgctcccaac gtcaggacga agtctccggg ctgtaaatga
    26101 ggcaacacct gatcgatgag ctggtccttt cgctcgacaa atgtcacgga aggatgaccc
    26161 gcggcacgga ttgcgtccgc cagtttggct cccgacacac cgggaatcgg ctgctcgctc
    26221 gccgcataga tctccgtgag aaacagctgg tcggaatcct tgaacgcctg cgcgaaatca
    26281 tcgagcaagt ccctggttct cgtatagcgg tgcggctgaa acagcacgac aagccgccgg
    26341 tccggccatc cctgctttgc cgccaccagc gtcgccttga tttccgtcgg gtgatggccg
    26401 taatcgtcca ccaccatgat gccgctcgct tctccgcgaa gatggaaccg tcgctccaca
    26461 ccgttgaatg ccgccaaggc ttttctgatc aggtccaccg gcacgtcgag ttcgatgccg
    26521 atcgcaatcg ctaccagcgc gttggacacg ttatgaatcc cgggaaccga aagccgaaat
    26581 ggcccgaggt tgcgcccccg gaaaaacgct cgaaactccg caccccattg cttgacgctg
    26641 atgtcggtcg ctctgaaatc cggcgtcatc ccctcgcgct cgtgcaatcc gtaggtgtga
    26701 tagcgcttga cgatcttcgg aaagagcgcc cggagccggt catcgtcggc gcacaacacg
    26761 gccaaaccat agaagggcac cttgttgatg aattcgagaa agctctcgtt gatccgttcc
    26821 atgctgccgt agtggtccag atgctcccga tcgaggttcg tcacggccac aatggtgggg
    26881 gagagccgca ggaaggaccc gtcgctctcg tcggcctctg ccacaagcag ctcgcctcgc
    26941 cccaatcggg cgtgactgcc caaggcattg accttcccgc caatgaccat cgtgggatcc
    27001 aagccgccct gcgccagtac gttggctacc attgaagtcg tggtggtttt cccatgcgcg
    27061 ccggcgatcg ccacgccgaa cttcaagcgc atcaactccg ccagcatttc ggccctcggg
    27121 atcacgggaa tctgcttcgc cttggcgacg agcacctcgg gattctgcgg cgagacggcc
    27181 gacgagatca caaccacctg agcctcgccg acgttcgatt cctggtggcc gataaaaatg
    27241 cggccgccca actcttccaa gcgccgcgtc gtgtcggacg cctgcaagtc agagccggtc
    27301 actttgtatc cgagcgtcag cagcacttcg gcgatgccgc tcattcccga cccgccgata
    27361 ccgactagat gaatctgttg cgtcttgcga aacatgggta tacgcctcag cccacgaacc
    27421 ggctgtgaag gatccaatcc tgagttgctc cgttgcccga caacattaga ctcctgtcgc
    27481 tccaagaggc tgattgatgt catggtggtc tcctataagg gcacaacatt cgcggacgat
    27541 tgattcggcc gcgtcggttc gtctcaatgt cgcacttgca gcacccatcg ccctcatccg
    27601 ttcaggattg tccaagatcg cggtgatcgt ttgcgccagc ttcggtccag tcagctccga
    27661 ttggggaaga agaacggccg cgccggctgc ttccatgacc ctcgcattct tcgtctggtg
    27721 gtcgtagatc gcagtcggca gcggaatgag aacggctggc ttgccacacg ttgtgagttc
    27781 ggccaccgtc atcgctcccg ctctcgccac cacgagatcc gcagcttgga tggccgaggg
    27841 catgtcatac agaaatggcg tcgccgtcac ggaaactccc gcccggcgat aggcttccgc
    27901 cacgcgcgca tgatccgact ccccggtttg atgcgtcacc gtcatctgcg ggcgctctct
    27961 aatcaattcg ggcaatcctt ctatcgccgc actgttaatc gccttcgcgc cctggctccc
    28021 gccgaagatc agcagatgcc gtcggtccgg ctcacgcgag ggtgcgtgcc cctgcttcgc
    28081 gcgctctaaa aattcccttc gaatcggcgt ccccaccact cgaaccttgg ctcgatcgaa
    28141 cgaagcggcc gcggactcga aggcgagaaa tacacgctgc acgtacgggc ccacggcctt
    28201 gttggccaaa cccggattcg cattcggttc gagaatcact cgcgcgacgc cggccaatgc
    28261 cgccgccatc accatcatcg ggctggtata gccgccgacc ccgatcacga gccggacctg
    28321 ccgcgcacgc aagatacgac ggcattgcca caaactgagg ggcatcgcgc aaagcccctt
    28381 caccgtaccc aacaggccag ttcccatcac cggcttggcg tcgattaacg ccaattcgaa
    28441 tccttcgtga gccaggacct tggcctcgag cccccgcgac gtgccgacga acagcacctt
    28501 tgtcgatgga tcgcgccgga gaaactcccg ggccaatgcc acggccggat agagatggcc
    28561 acccgttccc ccggctgcaa tcacgattgt catcagtgat ccgactcacc acgtccgcct
    28621 ttagatccgc cggcatgccg gtcccgcgaa atgctgagca atattccgac ccccacacaa
    28681 ctcaccacga gcgacgagcc gccgtaactg acgaacggca acgtcaggcc tttggtcggc
    28741 agaagtcccg ttacgaccgc cgcattgacc agcgcttgca cgccgatcaa cagggttatt
    28801 cccatgccga gatatttgcc gaacggcatg cgtgcgcgcg ctgcaatctg aaatccgcgc
    28861 cagacgaaga cggcgaaaag gagaatcacc gtggcggtgc cggccagccc caattcctcg
    28921 ccgaccagcg ccaagacgaa atccgtatgc gcttccggaa ggaagaacag tttctgcttt
    28981 ccctcgccca gccctacgcc gaagggaccg ccgctgccga acgccaagaa cgactgcgtg
    29041 atctgaaatc cggcatccga ggcgtctttc cagggagcca gaaacgtcat caagcgctgc
    29101 cgccggtagc tggagcccag cacgaggacc aggacggccg ggatagcgca gagccccagc
    29161 gcgaagagat gagagattcg cgcaccgccg aggaagagca agccgaccgt cacaagtccc
    29221 atcaccacaa ccgtcccgag atccggctcg agcaggacca ggccgctgag cacaccgacc
    29281 acgatcaaca ccggcaggag tccgctgcgg aaagccgtga ccttgtccga tttcttggtc
    29341 agataggctg caagatagat caccgtgacc aacttgacca tctcggccgg ctgaatcgaa
    29401 atgggcccta agcgcaacca gcgacgggcg cccttcgccg ccacgcccaa ggacggaacc
    29461 agcaccatca cgagcagaat caccatgcat ccgaggattg gaatcgaaag cttcttccac
    29521 agcgtgtaat cgatgcgcga cgtgaggtgc atgagcaaga aaccgaatcc gagccaggca
    29581 atctgacgct tgagaaagaa ttcgggatcc tgaaaccgat tgcccgccac gatggcgctc
    29641 gcgctgaaga ccatcactaa tccgaccaag gcaagcgtga gcgtcacgaa gagcagcgta
    29701 tggtccatcg ccacgcgctg cttcccaggc cgttgaccgg ccgtcggcca gggcaacgac
    29761 aaagtccctg cggatcgaga cgccatcgct cagaatcact cctcctcgtt cgctcacgtt
    29821 acgccggcaa cgcttggact gcggacttga attgccggcc tcggtcctga taatccgcaa
    29881 acatgtcgaa gctcgcacag gccggcgaca tcagcacgac atcgcctgcg gtggcctccc
    29941 gcgcggcgaa ctcgacggcc tcgcggaggc tgcccgcctc actcatcgcc gtggcgcccg
    30001 tccacgcctg cttgagcaga ggcgccgcct ctccgatcag aatcacgtgt ttcacccgtt
    30061 gacggacggt ctcaatcaat cgcgaaaaat caccgccctt gtcgcgcccc ccggcaatga
    30121 gccagatggg atggtcgata ccctctaatg ccttcaaggt ggcatcgaca ttcgtccctt
    30181 tggaatcgtt gacgaaacga acgccgcgac gttccctgac gagttccaga gcatgctcga
    30241 ggccgggaaa cgtggatacc acgtggtgaa tcacctccgt tggacagccc cacagggccg
    30301 cataggtggc tgcggccatg acgttctcca cgttgtgatg ccccagcatg cgaatgtcgc
    30361 tcttcctgca aatctcctgg cgcgagccgg tcaccgtggt gacgatcctg tcaccatcca
    30421 gaaaggttcc tccgtccatg tcggacgcga tcgcctgcgt tctgctaaag cccagacgct
    30481 tggcgcgaac gtggtgccga agtcccgcaa cacgggcgtc gtccagattg aaaagggcaa
    30541 aatctgcggc cgactgattt tcaaaaattc tccgcttggc cgagaggtat tcatcgatgg
    30601 agtcatagcg gtcttgatga tcgaccgtca cattgagcag cgtcgcgatc cagggatgga
    30661 actggtcgat cgtctccaat tgaaaactcg agacttccac cacgagatag tcgaacggat
    30721 tgggcttgcc ttgtttcgtc gcttgcagcg tcgtccaggc cgcttcgctc aatgcagttc
    30781 ccaggttccc cccgacgaac gcgcgtttac cctgctcggc caacatcttc ccgatcagcg
    30841 tgacagtcgt gctcttcccg ttcgtgccgg tgaccgccag aatcggcgca gacaggaagc
    30901 gtgacgccaa ttcgagttcg ctgatgactt tgacgccgcg ccgtcgcacc cgctcgagcg
    30961 cctccaatcg atatggaacg ccgggactga tcaccacgag atcggcatgc tccagagagg
    31021 tttcgtattg cgtgccgacg ctcacgcaga tccggcttcg atcgatcttc tcaaggaccg
    31081 cggtcaattc acctgcctct tttcgatcgg ccaccgtgac ccgcgcaccg gcctcctgca
    31141 gcagatgaca agcggcgacg ccgcttctgg ccattcccag caccgtcacc tgagcccccg
    31201 caagatccac tcgatcgacc tccaccattc tcatcaccgt aatttcagcg tactcagact
    31261 caatagagcc aggagaatcg caatgatcca gagccggacg acgactttcg gctcctccca
    31321 ccctttcatc tcgaaatgat ggtgaatggg cgccatgagg aaaatgcgct tgcctctgga
    31381 tttgaacgac accacttgaa agatcaccga gaccgcttca atcacaaaca caccgcccac
    31441 catcagcagc agcaactcat gcttgctgat gaccgccacc gtgccgagag ccgcgccgag
    31501 cggcagcgag cccacgtccc ccataaagac tgtggccgga taggtattga accagaggaa
    31561 gcccagactt gatcccagaa tggcagccgt aaacacggcg atctcaccgg caccttctat
    31621 atgggggatg agcaggtagt cggacatgat gcggtggcct gcgacatagg ccacgatggt
    31681 gtaggccagc gccgcgatca tgatcggtcc gatcgcaagc ccgtcgagac cgtccgtcaa
    31741 attcacggca ttggaactcc cgacgatgac gagcacgacg aacacgacgt agaaccagcc
    31801 aagatccggc atgaagttct tgaaaaacgg cacgttgagt ttcgtgctgt agccgggaat
    31861 gaaataataa agaacgatcg caatcaggaa agcgacgccc acttgcgccg caaacttctg
    31921 cgtgggcgac aatcccttcg attgcgcttt gataaacttg aggtagtcgt ccgcaaatcc
    31981 gatggctccg aatcctaagg tcgcaaccat caccagccag acgtacggct tcgtaatgtc
    32041 agcccacagc aatgtcgaga gaaacaccgc aaagatgata agtaatccgc ccatcgtcgg
    32101 cgtcccgctc ttcgcctgat gacgtttggg tccgtcatcg cggatctgtt gcccgagctt
    32161 gatttcctga agcttgcgaa tcagccatgg cgtcaacacg aaggcgatga ggaaggccgt
    32221 caccgctgcg tagatgatgc gaaagctgag gtaccggaaa acgttcagaa atccatattc
    32281 cgtgtgaaac ggatacaacc agatgtaaag catgcaccct ttccgtagag accgcgcccg
    32341 cttcgctacg acgcctgctt cgtcaccgct ctcataccgg tcaacacttg cacgatttgt
    32401 tccatcttca tgccgcgcga agctttaacg agaaccacat caccctgtcg gaccattctc
    32461 cgcaaaagct cggcagcccc cgccgcatca ggagtttcga tgatggccga ttccgacatt
    32521 ccggcccggt tcgccccttc ggctatctca tgtcccagcg gtccgcatac gatcagccgc
    32581 gagagatgca accccgcaag aaactggccg acctcacgat gcatgtgctg cgtatcccgc
    32641 cccagctcca gcatgtcccc caagaccgcc acccgctcac gcgccgggct ccactgagct
    32701 aacagctgaa gcgccgcctt catcgaagca ggattggcgt tgtaacaatc gttgatgatc
    32761 tgcactccgt ggtgcgtcac aacctgggag cgcatcgccg caggccggaa tctcgccaac
    32821 ccctgggcga tcgtggcgcc agaaaggttc agtgcgaacc cgacggcagc ggcggccaag
    32881 gcattggtga cgttgtgcag gccgtgcacc ttgatcgtga ctaccgtatg gcggctctta
    32941 ccgggaagca ggagacgaaa ggtagttcct ttccgcgggt cggacgacaa attcgtcgcg
    33001 cgcacgtctg ctttttcaga cagtccgaac gagacgactc ggcattgggc gcgtgccgcg
    33061 agatagtcga aataggggtc gtccgcattc aaaatcgccg tgccgtttgc cggaagcagg
    33121 tccaacagtt ccgccttggc ctgcgcggag ccctccatgc ttccaaaaaa ttccagatgg
    33181 tccggaccga tattggtgat gatgccgatc gtcggcctga cgatctcgct cagcctcgtc
    33241 gtttgtccct gttgatcgac ccccatctcg ataacggccg cctgatgtcg agccgtcagg
    33301 cgaaacagcg tatgcggcac accaatgcgg ttattgaaat ttccttccgt cttcagcgta
    33361 cgccaacgct gcgcaaggac actggcgacc atctccttcg tcgtcgtctt accgttgctc
    33421 cccgtcacag cgacaaccgg aatctcaaat cgactccgat ggtgcgtggc aagttgttgg
    33481 taggcaaaca gcggatcacg gacgccgaag aggaacggtc cggtgcgccc ggccgtcttg
    33541 gcgcctgggc ccttgccatg gggcaaccga tactcatcat ggacgatcgc gccgacagcg
    33601 cctttggcca gcaccgccgg gacgaattca tgaccgtcga atcgttcccc gctcagcgcc
    33661 acaaagagat cgcctcggcg aatcgaccgt gaatcggtac tgatgccccg gatgcgtcgc
    33721 ttggctgacg gcgagggatc gcctgccaac accttgacgc tgatgacttc gcgcagttct
    33781 tcaaccgtaa agagcgccat gggctcctcg ctctgctccg ctctggaccc cgaatgcagt
    33841 gtcacgcgca attcctcaac tgctggatcg cttcccgtgc cacctcgcga tcgtcgaagt
    33901 gaaatttttt cgtgccgata atttgatagt cttcatgacc cttgccggcg atgagcacga
    33961 tatcgcccgt ccgagcgagc cgcaccgccg tcccaatcgc ctcgcggcgg tccggcacga
    34021 ggtgatactc cacgtgagtg cggccctgca gcgcttcgcg aacgccggct tcgacttcct
    34081 gaagaatcgc catgggatct tccgtccgcg gattgtcgga cgtcagaacg acgacgtcgc
    34141 tgtgctcgac ggcgacgcgt cccattttgg gccgcttgcc gcgatcgcga tcaccgccgc
    34201 agccgaacag ggtaatgatt cggttggtct tcaacgcctt cgccgccgtc agcagccgga
    34261 ggagcgcgtc ttccgtatgc gcgtagtcca cgacgacggt aaaatcctgg cccgccgaca
    34321 cacgctcgaa acggcccggc acattagaga cctgcacgac agcttcgcgc acttgatcgc
    34381 aggtcgcccc ggcatgaagg gccacgccta tggctcccag caaattatag acgttgtgtt
    34441 cgccgacgag tcggctctcc acgctgaacg tcccggcagg ggtagccgct gtaaacgtcg
    34501 ttccgttcat ggaaaggcgg acttgctcgg ctttgagatc ggccctgttc gtgacagcgt
    34561 agccccacac cggcacgcga caggctgcac gaacctgaga tccgtaagga tcatccatgt
    34621 tgacgatggc ccgctggccg gctttcttcc cgccgccgag ccccgcgaac agtcttagct
    34681 tcgcctggaa gtactcctcc atggtccggt gataatcgag atggtcttgc gtgaggttcg
    34741 taaaaaccgc cgcgtcatac tcgcagccgg ccgtacgatc catcgccagc gcgtgggaag
    34801 acacttccat gacgacgcta ttcagtcccg cctgtgtcat ctttgccaac agtccctgca
    34861 attcgagagc tccgggcgtc gtgtgagaag cgggaatggt ttccgatccg atttgatagg
    34921 ccacggtgcc gatcaatccg acccgccgac cgattccttc caaaaaggcc ttacagagat
    34981 atgtcgtcgt cgtttttcca ttcgttccgg tcacaccgat catggcaagc cgcgcggaag
    35041 gatctccgta aaaccggctg ccgatcagcc ccaaggcctt gcgtgaatcg gccacccgca
    35101 cgaacggaat cgatccggtt tcaacagctt cttgcgcgac gatcgccgcg gctcccgcct
    35161 gcaccacctg aggcacaaag gtatgaccat ccgcacgctc gcctttcacg gccacgaaga
    35221 ggctgcccga cgcgacggct ctcgagtcgt ccgtgaccgc cgtcacggcg cgatccaatc
    35281 cgccatgctg ctcgacgatc tccacctgtc ctttgagtgc cgccaccaat tgcccgaccg
    35341 tcatagaccc gcgttccatc tccgtcgtta ctctcgtgcc accatggcga tcttcacttg
    35401 atcatcgcgc gacactccca agtaggtcag cacttgctcg ccgaccctcc gaaacaccgg
    35461 cgccgccacg gttccgcccc aggcttcccc ttggggttcg tcgattacca cgatcatggc
    35521 cagccgtgga gaatcggccg gcaggaaacc gacaaacgat ccgacgaact gggtgttcga
    35581 gtaggtgccg gttcg
//
LOCUS       DATIFR010000051        25916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2137968, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000051 DATIFR010000000
VERSION     DATIFR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137968"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /locus_tag="VLD60_02830"
     CDS             complement(<1..2426)
                     /locus_tag="VLD60_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HSB43915.1"
                     /translation="MKQQHVEHVRLMPCLVFGFILVLLFAWLTSLVHAASPITQSGLN
                     TRVSDPFLTAGGKTQYDITGGTRAGTNLFHSFGDFNVPTNNIANFLNTPINGVFPETS
                     NILGRVNGGNPSVIFGMIQMTNFGNANLFLMNPYGFLFGANATVNVGGMVSFTTADYL
                     RTQGTGGNGIFYADAAQMSVLTSAPVAAFGFLGSNPAAIAVQGSTLTVTPGQSISLVG
                     GNKGFAYTDPDTGLVAPAPVPGGVTMTGGKLLAPGGQINIASVAGPGEIFAVDFMPTA
                     GTTMGNISLSQGALLDVSADAAGTVRIRGGQLVMDTALISADTIDANGAPVAIDINVT
                     ENVSVSNTDVPALTARTTGAGNAGEITISSNSMDVTSTFNEATFFFPVIDTHTSGSGN
                     AGNVSINTGNLTVTGDPFGASWFIDSGTSGPSPGRGGDVSITANTIDFETTAINTGNL
                     FAFFLGEGGAGTAGNVTITADKLQMNFSQIVTDSNDFVTPIGTSGDITIAAREVNLFG
                     TGLSTTAYDHSGNIRITADQFIATESQIQAITTFRSGGEIRITGNVIEFREGSNIASS
                     TMADGDAGPIIISASDHVAFLESSPGGRPGGIFNNSFGDRGSLGNAGDVVITTPNLQL
                     TGGARINTTTASSGRGGNVTINADSISMSGENSGFEIESLFNLGTTQSSGIYTRTIGG
                     NCIGPCGNAGNISITTGSLTMGNGAQINSGTSSSGQGGNITINAANTIGMSGTLTTGQ
                     PGGIQSRTIGTDPGAGAGGNISLIAGQSVSISNGAAVSASTTGLGNAGNILVKANDVT
                     ISGRGTITAAS"
     gene            complement(2932..3009)
                     /locus_tag="VLD60_02835"
     tRNA            complement(2932..3009)
                     /locus_tag="VLD60_02835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2971..2973),aa:Glu,seq:ttc)
     gene            3168..3659
                     /locus_tag="VLD60_02840"
     CDS             3168..3659
                     /locus_tag="VLD60_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43916.1"
                     /translation="MMAPLTRASWGGALVLISSLLLAHCAHDTYQERANLINDHVEAF
                     YAHLKANRVDSAIRENEQIEAMASDMAQTVKKRAAQQGTTQAEREFALMKTANEAAAA
                     NWLALGQYFAIKKQYPQARATYQRVVNTYTNPTDRTYREQAARALKDLDIVNPPIGST
                     SNP"
     gene            3839..4213
                     /locus_tag="VLD60_02845"
     CDS             3839..4213
                     /locus_tag="VLD60_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43917.1"
                     /translation="MPGITLLEWPAKDLRTATINYIHNRRTFSSVSEDQGSLTLTIKA
                     WLWMRSRGEYRYTIRLESDLGPSGKPPKKSYLAQKEAVGSLVRWVTASDRDPIEEAVQ
                     SALDELLMLIEEDGALYGKRSN"
     gene            complement(4503..5210)
                     /locus_tag="VLD60_02850"
     CDS             complement(4503..5210)
                     /locus_tag="VLD60_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HSB43918.1"
                     /translation="MAELRVINLITNSSRRSAGTDRLPITVHLATGLEKIGLAMKSRT
                     WRREGRAGLGPLQRQVLALLRSKPDQSAQVSAIAEELVVRLPTASEAVATLERKRLVK
                     RRRSMRDGRIVTVELTARGLRACAPAGGMPNHLAAAVGALSTHEQVSLLKSLMKVIRA
                     LQDRGEISVARMCLSCRYFRPHQYEDAEQPHHCDYVNAPFGDQALRLQCAEYEPASVS
                     QARDLWTKFTGDAQAAG"
     gene            5622..6236
                     /locus_tag="VLD60_02855"
     CDS             5622..6236
                     /locus_tag="VLD60_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43919.1"
                     /translation="MRIDAHLLFGTVFGAALLISPLAWADGAGQCQRHSGTSHVMSGH
                     GGHGHSGVSAHMLRHLLKNKQELGLTAEQVTKLRAVALDADRAGIRAEAEVLVSGREL
                     RALLWDEKAELSSIEAKVKEHEALEATARIISIRAKRELIGVLTPEQQGKQKALWEQH
                     RQHDRRHMMRAEVEQGINEAEKIEASTDVSGVKISEMESAPSAS"
     gene            6309..6944
                     /locus_tag="VLD60_02860"
     CDS             6309..6944
                     /locus_tag="VLD60_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB43920.1"
                     /translation="MTAPLGLAGFYLPMTDEAFTGLKSEPGDGDLLERIRRGEIDRFA
                     DLISRYHSHVIRIVGRRVPADRAEEIAHDVFVRAYFGLAQFSGSTSFDHWLAGIAVRT
                     CYDFWRGRSREEVPVSALTHEHHQWIEQMLAAQSADQFYNQVRTREAVEVLDWALAQL
                     SPENRAVLTLVHLDGLSVREAAQLLGWSVINVKVRAHRARQALRKILINRH"
     gene            6955..7272
                     /locus_tag="VLD60_02865"
     CDS             6955..7272
                     /locus_tag="VLD60_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43921.1"
                     /translation="MASNDENLQNLERALIETYRARPDLPVERARLTQDVMRDIRQTT
                     CESGWWSPSIALDQFVWRTATIAASVVLVVTVFTVAVIRTTAGESAVSLAEEFESTPL
                     LGE"
     gene            7299..7718
                     /locus_tag="VLD60_02870"
     CDS             7299..7718
                     /locus_tag="VLD60_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43922.1"
                     /translation="MSRVKLWAGLVVLFGAGMLTGVVGTCLYHERERAHGGERGPAAQ
                     HERIMKRLTQELSLSATQQAEIEPIVTRAHVSILELRFAHQPEIEQILARGIAELKEK
                     LSAEQQPALDRMYAQLQQRWQASRDYTEAKKKERTQQ"
     gene            7759..8988
                     /locus_tag="VLD60_02875"
     CDS             7759..8988
                     /locus_tag="VLD60_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HSB43923.1"
                     /translation="MADSIHFSTMLKRWVFGLCAACFLALGGYALLAKSGEEPSRAGQ
                     VPSGARAFPVVTATAKTGDIGVYLNGLGSVVPLNTVNVKSRVDGQLMKVLFQEGQIVH
                     SGDLLAQIDPRPFEVQLNQAEGQMARDQAQLKNARLDLQRYKELLEQGFIPKQQLDTQ
                     EAMVRQLEGIVKADQSQIDNAKLQLVYSHITAPISGRVGLRLVDAGNMVRANDNNGLL
                     VITQLTPITIIFTIPEDSLPGVLERLKSGQQLTVEAFDREQKHLLATGTLLTVDNQID
                     SNTGTVRLKAVFPNEDGALFPNQFVNARLLLDVKHGVTIIPSAAVQRGAKGTFVYVVK
                     DDKTVAVRPVTIGTAHGENTSIDKGLSPDELVVVDGTEKLREGSKVEARNQSDKPAPE
                     PETSPAGSEKPRRGPRP"
     gene            8985..12101
                     /locus_tag="VLD60_02880"
     CDS             8985..12101
                     /locus_tag="VLD60_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HSB43924.1"
                     /translation="MNPSRLFILRPVATSLFMVAILLAGALAYRQLPVSALPQVDYPT
                     IQVLTFYPGASPDVMASSVTAPLERQFGQMPGLNQMTSTSSGGSSVVTLQFSLDLSLD
                     VAEQQVQASINAASTFLPRDLPNPPVYNKVNPADAPILTLALTSNTLPLPQVEDLAET
                     RFAQKISQLSGVGLVSISGGQRPAVRIRANPRALSAYGLTLEDLRATVAAANVNQAKG
                     SFDGPRRASIINATDQLLSSRDYQPLIVVYRNGAPVVLSDVAEVVDDVENVRQAAWMN
                     EVPAILVNVQRQPGANVIEVADRIKKLLPQLQSALPSSVQVTVLTDRTTSIRASVHDV
                     QFELMFAVALVIMVIFLFLRTLSATVVPAAAVPLSLVGTFGAMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENISRYIERGESPLQAALKGSEQIAFTILSLTISLIAVLIPLL
                     FMGDVVGRLFREFAVTLSITILISAVVSLTLTPMMCARLLHHRRQSEQSTVYRWSQQL
                     FDRTIEAYGRTLRWVLNHQPATLAVAIGTLVFTIALYIIVPKGFFPVQDTGAILGISE
                     AAQSISFPAMVERQQALARTILADPAVASLSSFIGVDGTNTTLNSGRIQINLKPIAER
                     RMSVQDVILRLQGQLAGVEGITLFLQPMQDLTVEDRISRTQYQYTLEDADPEELSRWA
                     PTFAEALQALPELRDVSSDQQDRGLASLLVIDRSTASRLGITPQLIVDTLYDAFGQRQ
                     VSTMFTQLNQYRVILEVIPEFQNGPQALQFLDIRSSTGGQVPLNVFTQFSETTTPLTI
                     SRQGQFPAVTLSFNLAPGTSLGDAVTAIERTARAIDLPASIRGSFQGSAQAFQASLGN
                     ETWLILAALVTVYIVLGILYESYVHPLTILSTLPSAGVGALLALMLFQTEFSVIALIG
                     IILLIGIVKKNAIMMIDFALDAERKEGKQPLEAIYEACLLRFRPIIMTTMAALLGALP
                     LAIGSGVGSELRHPLGIAIIGGLILSQALTLYTTPVVYLAFDRLARRGRSRRSSAPTT
                     EALTPGTV"
     gene            12098..15226
                     /locus_tag="VLD60_02885"
     CDS             12098..15226
                     /locus_tag="VLD60_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HSB43925.1"
                     /translation="MNISAPFIQRPVATTLLTFGVTLVGIVAFRFLPVAALPQVEFPT
                     ISVSANLPGASPEIMATSVAGPLEHQFTRIAGVTEMTSTSMLGSTSITLQFELNRDID
                     GAARDVQAAIMAARGDLPANLPYRPTYRKINPADAPILILSLTSDIVSRGRMYDVASS
                     ILQQKLSQIDGVGQVYVGGGSLPAVRVDLNPTALNKYGIGLGDVRRMLSSTNVNRPKG
                     QLADGTRTWEIRTNDQLHDAEDYLPLIVSYRQGRAVRLSDVATVEHSVEDLRTMGVAN
                     GAPAVLIIISRQPGANIIETVDRVRAILPQLEASIPGSMTLSIVGDRTPVIRASLHDV
                     ERTLAISVLLVILVVFLFLRDVRATLIPCVAVPVSLISTFGVMYLLGYSLDNLSLMAL
                     TIATGFVVDDAIVVLENISRYREQGMAPMQAALRGAREITFTVLSMTLSLVAVFLPIL
                     FMGGMMGRLFREFAVTLSVAILVSLVVSLTTTPMMCARVLRSDQGRSHGWSYRLIARL
                     FESMRGGYARSLTWVLHHPRGMLALTLVTMAVSIYLYTVVPKGFFPQQDTGRMFGNIQ
                     AAQDISFQAMRQKLTEVVDIIKSDPAVETVTGFSGGGGNTNSARMFITLKPLQERQIS
                     VDQVIARLRPKLAKVPGAPTVLQAIQDLRIGGRASSAQYQYTLQGVDLAELNMWAPRV
                     ERQLRTLPEIADVNSDQQDKGLQSLVVFDRSTASRLGLSPQLIDDTLYDAFGQRQVSI
                     LYTPLNQYHVVMEVAPQYWQNPSTLQEIHVRSSTGAQVPLSAVTRFEPTNTLLMVNHQ
                     GQFPGVTLSFNMAPGTSLGEAVQAIEKSMHEIGLPASIRGNFQGAAKAFQASIENQPL
                     LILAALITVYIVLGILYESYVHPLTILSTLPSAGVGALLALLLFKTELSMIALIGIIL
                     LIGIVKKNAIMMIDFALDTERKNGKRPEEAIYEACLLRFRPIMMTTMAALFGALPLAL
                     GTGVGSELRRPLGIAIVGGLLVSQLLTLYTTPVVYLYLDRLRLRFLRITKGLREGTKQ
                     PPPPGTHQPGYS"
     gene            complement(15391..16113)
                     /locus_tag="VLD60_02890"
     CDS             complement(15391..16113)
                     /locus_tag="VLD60_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB43926.1"
                     /translation="MLLDVGNAKLETLVLLPGLDGTEVFFRPLLASFPEWVRPHVVSF
                     PPAGATEYADLLAIVRDAVSAIPSFYVLGSSFAGPLALMLAEAEPDKVQGVILATTFV
                     SPPRRIYVRMRFTSVAPTIWLLRTCRHIPAWLSRGPTDQLRLDTAEIWKRVTAHIVAA
                     RIRSLLNIDARELLRRCPAPVLCIAGSNDGVVPWRNVEEIVRVRPSVSVRRIKGRHFA
                     IYTNPTATMEAITAFMETKSVP"
     gene            complement(16187..16534)
                     /locus_tag="VLD60_02895"
     CDS             complement(16187..16534)
                     /locus_tag="VLD60_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43927.1"
                     /translation="MLLKKCLSLCLIACFLYVATPILIMAESEQVFQKVSANGQRNLR
                     PFTAKDKWEIQWDAKGTLLTITIFTTDGKLSTVAATQEGPGSGSSYQPKGGEYYLQVS
                     GTGEWTITVVQLP"
     gene            complement(16658..17110)
                     /locus_tag="VLD60_02900"
     CDS             complement(16658..17110)
                     /locus_tag="VLD60_02900"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HSB43928.1"
                     /translation="MAFTIQSYRRFLPLFHCSGVWSLITILLLSVEPVDAEWVDVGGK
                     VEEGLTVYTVYVDPDTIRRNGDVVTLWALFDYKTIQSIVGGPWLSSKARREYDCVKER
                     VRLLGFTTFSGNMGSGEAVYSNLDETKWEPVAPDSLDRNLWEVACSMK"
     gene            17407..17772
                     /locus_tag="VLD60_02905"
     CDS             17407..17772
                     /locus_tag="VLD60_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB43929.1"
                     /translation="MMTRQTQGAITDRHDKAVVQDRGGRRAELSCRLFFFGDDDFEGE
                     GTLLDISTRGCRASSLIAVQPGMLLKLSVFLPDYLWPLRVDEAMVRSVNGQEFAVEFT
                     SIRLAQRERLRALVMKEKP"
     gene            17816..18178
                     /locus_tag="VLD60_02910"
     CDS             17816..18178
                     /locus_tag="VLD60_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB43930.1"
                     /translation="MSKILVIDDEESVRMLLRAVLEEDGHEVREATDGRTGIALYRQE
                     RADLVIADILMPDLNGLDMIVELTREFLDVKVIAITGVYGEPDLLKRARLLGARHTFY
                     KPFSPEELLKVVRYELTH"
     gene            complement(18452..18865)
                     /locus_tag="VLD60_02915"
     CDS             complement(18452..18865)
                     /locus_tag="VLD60_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB43931.1"
                     /translation="MSDGITILFVDDDARQYEFCVGQLQGSSSNYHIVFAATGRAGLD
                     ICARQPIDCVVLEIDLPDVSGFAVLSTLVPRASHPEIAVIVLTRLPNPFLLELAIKSG
                     AQAAFHKTTVSGDQLEKSILKAISTVRKRLNGNFS"
     gene            complement(19048..20796)
                     /locus_tag="VLD60_02920"
     CDS             complement(19048..20796)
                     /locus_tag="VLD60_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSB43932.1"
                     /translation="MTTAAEVLIDRLIDWDVSVIFGLPGDGINGIIEALRTRRDRIRF
                     VQVRHEESAAFMACAYAKYTGRLGVCLATSGPGGIHLLNGLYDAKLDGQPVLALTGLH
                     FHDLIGTHAQQDVELDKLFLDVCVYNVRIMGATHVESVTDLACRTALSYRGVAHITFP
                     VDVQEQDAQRDGSKRNVPHHTSEHFARSARLPDSQDLSRAADLLNAGQRVAILAGRGA
                     LNATDELEETAEILGAPIVKALLGKAAVPDDSPYTTGGVGLLGTKPSQNVLEQCDTLL
                     MVGSSFPYIEFLPKPNQARAVQIDLNPARIGLRYPIEVGLVGDCRRTLQLLHPLLQPK
                     QNRDFLQQAQNEMASWREMMKEIGTRPDKPMKPQVVAWELGKKLSANAIVSADSGTIA
                     TWWARHIPAKRGQMHSLSGNLASMANALPYAIAAQIAYPDRQCIAFVGDGGFSMLMAE
                     FATCVKYKLPVKVVIIKNNSLGMIKWEQMVFLGNPEFGCDLQPIDFTLFAQACGGTGL
                     RIDDPADCGRVLAQALSTPGPVIVEAIVDPFEPPMPPKVTLDQAVHFWKALARGEPNR
                     VKIAMTVLSDKVRELV"
     gene            21279..22841
                     /locus_tag="VLD60_02925"
     CDS             21279..22841
                     /locus_tag="VLD60_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HSB43933.1"
                     /translation="MIPNPNYTACKALTLLIPMLAIIAGCSHTIPPANTRPQPPPQLG
                     KTPPVIEPLSASRKQLQAAESVLPVTLTVDLSPVQRAIQAAVPDQFVETNHPLAANYR
                     WRFVREGEPKVQIQDGLVKFQAFYRGEIESTAARACRLDPLYPILEGTGRLMLREQDQ
                     QLLVTMTDIQTTVSLKPESDSKCNMFNLPVKDQMAELIKQESLKQQIAQSIEQAGYTI
                     PIHLVWERLNEPMPMTVASANTQLCLYGKVKEFTVGSLKGPAQQTTISGLARETPVAI
                     YQSPCKQPSVTPVKVQVGSTAAAMAEGQPYNVLLSVPIPYAVLNHQLQDRLFHHEVRL
                     DTTFKDKILVEQVVVSDANGRVLFAVNTSGDLNGTLYYWGTPQLIDEGNRIVISDLQM
                     ANETKVALDDIKVGYWQLVDDQLRDRLQRAATLDLSQRFGNMKTAMSGQRQAGGLTTD
                     VLMGRQEAQRVYSTADALVVDILLQGTASAAGRVPVEQHARMVRIEPMPLDRDPVATT
                     PSPSAPIPADRP"
     gene            23274..24059
                     /locus_tag="VLD60_02930"
     CDS             23274..24059
                     /locus_tag="VLD60_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB43934.1"
                     /translation="MTNEERIQVFDSQGQAYKQAFQTFLDHTDQKRNAKRWLQQIVDG
                     LSTRKVFIDAGAGNGEVTRAFADDFERTIAIEPNIFLVKQLQEAVPTAEVEVIDEPIM
                     AAQPTARGDLVLCSHTLYYIPPEEWLCHLERLVSWMSPDGVTIVVLQHRETGCMNMLH
                     HFYGQRFEIRRTAEAFRVKHGELYDVMTTLDPAHVVTPDLEKTYAVAEFMLNLLEVKC
                     APARREVEAYVQAHFATGRGGYCIPVHQDFLQIRPLRRGEIIT"
     gene            complement(24498..24854)
                     /locus_tag="VLD60_02935"
     CDS             complement(24498..24854)
                     /locus_tag="VLD60_02935"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB43935.1"
                     /translation="MPRIPFQASINYLTPEIMAKGLVIDVSREGLQIESQEPVPIGMR
                     LALVLYLSNDQEPVMIEDATVQWAIGTHFGVKFVKWSTNAKARLNNLFWTGIQERCQS
                     LLHLMTEAAKASPLNA"
     gene            complement(25319..>25916)
                     /locus_tag="VLD60_02940"
     CDS             complement(25319..>25916)
                     /locus_tag="VLD60_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43936.1"
                     /translation="LNLSRSQAHAAGGGQTKFQRISTQFIAALGDPGATSGSGAQSWG
                     LWPLDPGPRGVELNSYTRLKDAGGVAPARWKFDGTDWWLEEHRLIMEQPTFPLPPGKY
                     VVTGNREVTAVLTIHPADRNGDRRWELDKGATLYDVTHLGCRSARYTPAAVGGSCSPA
                     NAQKTAFPVAPGGAMPPVEGCTKQDYAVLIVIGVGVED"
BASE COUNT         5695 a         7341 c         7193 g         5687 t
ORIGIN      
        1 gatgcagccg tgatggttcc tcttccacta atcgtgacat catttgcctt gactagaatg
       61 ttgccggcgt tgcctagacc tgtggtgctc gctgagacgg cggctccatt cgaaatgctt
      121 accgattggc cagctatgag agaaatgtta ccgccagcgc ctgcaccagg atctgttccg
      181 atggttcggc tctgaattcc tccgggctgg ccggtggtca gcgttccgga catacctata
      241 gtatttgctg cattgattgt gatgttgccc ccttgtccac tgctgctggt gccactattg
      301 atctgagctc cgtttcccat ggtcagagaa ccagttgtaa ttgatatgtt tccagcattc
      361 ccacacggtc cgatgcagtt gccaccaatt gtgcgggtgt agataccgct cgattgagtc
      421 gttcccaaat tgaaaagcga ctcgatttcg aagccggaat tctcgcccga catagagatt
      481 gaatcggcat tgatggtcac atttccccct cgaccacttg aggctgtcgt cgtattgatg
      541 cgtgcaccac cggtcagttg aaggtttggc gtggtaatca ctacgtctcc cgcattgccc
      601 aggctacccc tatccccaaa tgagttgttg aagatgccac ccgggcgacc acccggtgaa
      661 ctttccagaa atgcaacgtg atctgaagca ctaataatga taggcccggc atcaccgtcc
      721 gccatagtgc tcgaagcgat gttgctccct tctctaaact cgatgacatt acccgtgatc
      781 cggatttccc cgccggatcg gaaagtggtg attgcctgga tttgagattc agtggcaatg
      841 aattggtccg ccgtaattct gatgtttcca ctgtggtcgt aagccgtcgt gctgaggccg
      901 gtcccaaaca agttcacctc acgggccgct atggtaatgt ccccggaagt cccaatcggg
      961 gtcacaaagt cattggagtc tgtaacaatc tgggagaagt tcatttgaag tttgtcagca
     1021 gtgatggtga cgtttccggc tgttcccgct ccaccttcac ccaaaaagaa cgcgaacaga
     1081 tttccggtat tgatcgctgt ggtttcaaag tcaatcgtgt tcgccgtgat tgagacatca
     1141 ccaccacggc ccgggcttgg gccactagtt ccactatcaa tgaaccatga tgctccaaag
     1201 ggatcgcccg tcacagtgag attgccagtg ttgatgctga cattccccgc gtttcctgat
     1261 ccggaagtat gtgtgtctat gactggaaaa aagaatgtgg cttcgttaaa ggtgctcgtc
     1321 acatccatgc tattagaaga gatcgttatc tcgcctgcgt tgccagcgcc tgtggttctt
     1381 gcggttaaag caggaacatc ggtgtttgaa acagaaacat tctccgtgac gttaatatcg
     1441 atcgcgaccg gagctccatt cgcatcgatg gtatccgccg aaatgagggc cgtatccatc
     1501 accaactgcc cacctcgaat gcgtaccgtc ccggctgcat cagccgaaac atccagcagg
     1561 gcaccttggg agagactgat gttccccata gtcgtgcccg ctgtcggcat gaaatcgacg
     1621 gcgaagattt cgccaggtcc tgcaacactc gcaatgttga tctgtccacc tggagctaag
     1681 agtttgcccc cagtcatcgt gactccacct gggacgggcg caggggcgac gaggcccgta
     1741 tctggatcgg tataggcaaa ccctttgttc ccaccgacga gtgaaatcga ttggcctggt
     1801 gtaactgtca gagtgcttcc ttgtacggcg atcgcggctg gattggagcc caagaatccg
     1861 aaggccgcca ctggggcgct cgtcaataca ctcatctgtg cggcgtcggc atagaagatc
     1921 ccgtttccac cggtcccttg cgttcgaaga taatccgccg tggtgaacga caccatccct
     1981 ccaacgttca cggtggcatt ggcgccaaag aggaagccgt acgggttcat caaaaaaaga
     2041 ttggcatttc caaagttggt catttgaatc atcccaaaaa tcaccgacgg attaccgccg
     2101 ttcacacggc cgagaatgtt ggaagtttcg gggaaaacgc cgttgatggg tgtattgagg
     2161 aaattagcga tgttgtttgt tggaacattg aaatcgccga aactatgaaa cagattcgtg
     2221 cccgctctgg tgccacccgt gatgtcgtac tgagtcttgc cacctgcggt caggaagggg
     2281 tcactaaccc gtgtgttcag gccggactgc gtgatgggcg aggccgcgtg gaccagggag
     2341 gtcagccaag cgaataacag aacgaggatg aaaccaaata cgaggcaggg catcaatcgc
     2401 acgtgttcca catgttgctg tttcaagggc aaacctcctc ttggaattga tgctttcacc
     2461 ccgcaaatgc tggctgctgg atgatccttg aatgaggcgc atgtccatcc ccgacaggcg
     2521 tagggaggca aattctagga gtgccctgtc agcgaagcaa tagggccgaa tgggggtaat
     2581 taaatacgtg tatttggggg gccatcgatt gattcaaaaa aaatgcaaaa aaatctgaga
     2641 gcctatcacg agggctggga ttcgatttac ccccctagct ggcctccgca tggaagaaca
     2701 tgaaagcccg tttctcaggt tccagtgacc gccatacttc taccctgccc ttgaagtgct
     2761 gtctaatttt cttcggaatc tgaacacgcc gatagaacgt cttgcggtcg gatgaagaaa
     2821 aactgtagac atagcccctc ccaaaaccgt agctgaggtt gataagggct acagttcagt
     2881 gaagggctat tggctaaatc gccgctattt atgcgggtta cgtagtattg ctggtgtccc
     2941 caacgggatt tgaacccgtg ttgctggctt gaaaggccaa cgtcctaggc caggctagac
     3001 gatggggaca ggaggaaagt cgcatgaaac gagctggact tgtaccatac ttgaaccccg
     3061 ggtgtcacta gagtcccgac agtcccgtgc attcaccggt cgaattttct ccctcagata
     3121 gagtaggata atgcgcgtct ggaccgcgcc tgagaggaga agagaagatg atggcaccat
     3181 tgacccgcgc ctcatgggga ggagccctcg ttctcatcag cagcttgctg ctggcccatt
     3241 gtgcacacga cacctatcag gaacgcgcca atctcattaa cgaccatgtc gaggctttct
     3301 atgctcattt gaaagccaac cgggtcgact ccgctatccg tgaaaacgag cagattgaag
     3361 ccatggccag tgacatggca cagacggtca aaaaaagggc tgcccaacaa ggcaccactc
     3421 aagcagaacg agaattcgct ctcatgaaga cagccaacga agcagctgct gctaactggc
     3481 tggcgctcgg ccagtacttt gcgatcaaga agcagtaccc acaggcccgt gcgacctatc
     3541 aacgggtggt caatacctat accaatccaa ccgatcgtac ctatcgagaa caggctgcgc
     3601 gcgccctcaa ggatttggac atcgtaaatc cgccgatcgg ttctacgtcg aacccgtgaa
     3661 ttcccatcgt ttcgaacttc gtgtcacgct ctcgctactg tgcctcatag gcttatttgt
     3721 cggtggctgc gtccacaaga tacatgtggc cccgaatcct tcgcttcgct cagaatcgcc
     3781 gatcccagaa tccctccgag ttgaagtacc gtttcttgcc atggagggac cagaccatat
     3841 gcctggaatc acgcttcttg agtggccggc gaaggatctt cgcactgcga cgatcaacta
     3901 tattcacaat cgccgtacgt tttcctcagt cagcgaagac cagggaagct tgaccttgac
     3961 catcaaagcc tggctctgga tgcggtcacg gggggaatat cgctatacca tccggttgga
     4021 atccgacctc gggccatccg gcaagccacc caagaaatcc tatctggccc agaaagaagc
     4081 ggtgggatcg ctcgttcggt gggtcaccgc gtctgatcga gatccaattg aagaagcggt
     4141 tcaatcagca ctggacgagc tgctcatgct gatcgaagaa gatggcgcat tgtatgggaa
     4201 acgatcgaac tgaggaaccc ggtcgctaga ggattcgtcg ggatcgaatg atcagcaggg
     4261 cggcgaaact tcccacagcc aatccagcca taaagagcgc tcctgccatc caggccccag
     4321 cattgccgcc ggccggcgta tttcgttgac gcaactcacg acggagattg ggcatatgcg
     4381 attcagcccc aagccactcc tcggctgagg gagcggaagg ccgtcgaata cctgctcctg
     4441 ctgcatttct ttctaacaat tgactcacca tgtctctcac gtaggtttga gtctgtgccc
     4501 ggtcaaccag ctgcctgtgc gtctcctgta aacttggtcc acaagtcgcg ggcctgcgac
     4561 accgaagccg gttcatattc agcacattgc agccgcaacg cctgatcgcc gaatggcgcg
     4621 ttcacataat cgcagtgatg tggctgttcg gcatcttcat attgatgtgg gcggaaatat
     4681 cgacaagaaa ggcacattcg tgcgaccgaa atttctccgc gatcctgaag cgcacgaatc
     4741 actttcatga ggcttttcaa aagcgaaacc tgttcatgcg tggataatgc accaacggca
     4801 gcagcaaggt gattcggcat acctcctgcg ggcgcacagg ctcgcaggcc ccgcgcggtc
     4861 aattccacgg tcacaatcct cccgtcacgc atggaacgtc ggcgcttgac aagccgcttc
     4921 cgctcaagag tcgccacagc ctcggacgcg gtgggcaagc ggacgaccaa ctcctcggca
     4981 atcgcagata cctgtgcgct ttgatccggc ttggaacgga gaagagccaa gacctgacgt
     5041 tgcagaggac cgagtcctgc ccggccttcc cgacgccacg tgcggctctt catcgccaac
     5101 ccgatctttt ccaatccagt cgcgaggtga acggtgatcg gaagacgatc cgttcccgct
     5161 gatctcctcg aggagttcgt tatcagatta ataacccgca gttcagccat agggaccttt
     5221 caaaaatgca accatttcag tctagtgcga acaatatgcc ccgtcaaccc cccggagttg
     5281 gtacagcgag tgcggctagt ggaatcccgc ggcaattgac gaagctgcag aatgcgagct
     5341 cacaggtaca taggcagtgg aacgaactca ctgcgcaacc ccgtcgaacg aaagtgatga
     5401 aatcggttca ccgagctgag agaactgtgg tgagctacca gggacgaaac ctcagggccc
     5461 tcgcttcaaa cgcgccaaga tgtggatttc tcaggggctt ggtgttgcga aggctcctct
     5521 agacccccct gtaacctttt cagtgcctgc tgcgactagg acatagaagg aactgttctg
     5581 atccgttgag attggcagac tatttcacca aggagaacaa catgagaatt gacgcgcatc
     5641 ttcttttcgg gacggtattc ggagccgcct tgcttatttc ccctctcgcc tgggcggatg
     5701 gtgcaggcca gtgccagcgt cacagtggca ccagtcacgt tatgtcaggg catggagggc
     5761 atgggcatag tggtgtgagt gctcacatgc tgcggcatct gctgaaaaat aagcaggagc
     5821 tgggtcttac cgctgaacag gtcacaaaat tacgcgctgt cgccctcgac gcagatcgag
     5881 ccggcattcg tgccgaggca gaagtgctgg tcagcggacg ggaactgcgt gcgctgctgt
     5941 gggacgagaa agccgagcta tcttcaatcg aagcgaaggt caaagaacac gaagcccttg
     6001 aggccaccgc ccgtattatc agcatcaggg cgaaacggga actcatcggc gttctgacgc
     6061 cagagcaaca gggcaagcaa aaggctctgt gggaacagca tcggcagcat gatcgacgcc
     6121 acatgatgcg agctgaggtt gagcaaggaa tcaatgaagc cgagaaaatt gaagcgagca
     6181 cggatgtctc cggagttaaa ataagtgaaa tggaaagtgc cccatcggcc agttgaccgc
     6241 ccgaacgtgc ccgcggcaga caggatatcg tgcagtgtaa tggtgctatg atgagcggcg
     6301 tttcggtgat gacagcgcca ttgggtctcg cgggattcta tctgccgatg acggatgaag
     6361 cattcactgg tctcaaaagc gagccgggcg atggggacct ccttgagcgt attcggcggg
     6421 gggagattga ccgatttgcc gacctgatca gccgatacca cagccatgtc atccgcatcg
     6481 tcggtcgtcg ggtgccagcg gaccgtgcag aagagatcgc ccatgatgta tttgtcagag
     6541 cgtacttcgg gttggcgcaa ttctcgggct cgacctcatt cgatcattgg ctcgccggca
     6601 ttgctgtccg aacttgctac gatttctgga gggggcgatc gcgcgaagag gtgccagtca
     6661 gtgcgctgac ccacgagcac catcaatgga ttgagcagat gctggccgcc caatcagcag
     6721 accagttcta caaccaggtg cggacacgtg aggcggtaga ggtactggat tgggcactgg
     6781 cccaattatc tccggagaat cgagcggtgc tgactttggt ccatctcgac ggcctttctg
     6841 tgcgtgaggc ggcacaatta ttgggctgga gcgtgatcaa cgtgaaggtg cgggcgcatc
     6901 gggctcggca ggcgcttcgc aagattctaa tcaaccgtca ctgagggaac cataatggca
     6961 tcgaacgatg aaaacttgca gaacctcgag cgtgccctga tcgaaactta tcgggctcgg
     7021 ccagaccttc cagtcgaaag ggcccgtctc acacaggacg tgatgcggga tattcgccaa
     7081 acgacatgtg agagcggttg gtggagtcct tcgattgctc ttgaccagtt tgtctggcgg
     7141 acagcaacca tcgctgcctc agtggttctg gtcgtgaccg tctttacggt cgcggtaatt
     7201 cgaacgactg caggagagag cgccgtgtcg ttggccgaag aattcgaatc gaccccgctc
     7261 ttgggggagt aggaaatcgg gaagaggagg cgcgtaccat gtcacgagtc aaattatggg
     7321 ctggcctggt cgttctgttc ggagcaggaa tgctcaccgg agtggttggg acctgcctct
     7381 atcacgagcg agagcgcgcc catggaggag agcgtggccc agccgcccaa cacgagcgga
     7441 tcatgaaacg gctcacgcag gaactatcgc tctccgcgac acagcaagcc gagatcgagc
     7501 cgattgtgac ccgcgcccat gtgagtattc tcgagttgcg cttcgcgcat caacccgaaa
     7561 tagagcagat cctggcccgc ggaatcgcgg aacttaaaga gaagctctct gcagagcagc
     7621 agccagcgtt ggaccggatg tatgcccaac tccaacagcg ctggcaggcg tcacgggact
     7681 acacggaagc gaagaaaaaa gaacgtacac agcagtaagc tcatgtcgcg cgtggcaaga
     7741 caaaacgagt aagacaccat ggcagactcc attcattttt caacgatgct gaaacgatgg
     7801 gtgttcgggc tctgtgctgc ctgtttcctt gcgctcggcg ggtacgccct cctggcgaaa
     7861 tcgggcgaag agccgtcgcg tgcgggtcaa gtgccgagcg gagcacgcgc gtttcctgtg
     7921 gtgacggcca ccgcgaaaac cggcgacatc ggagtctatc tcaacggact tggctcggtc
     7981 gtgccgttga ataccgtcaa cgtcaagagc cgggtcgatg gccagctcat gaaggtgctg
     8041 tttcaagaag ggcagatcgt gcacagcggc gacctgttgg cgcagatcga cccgcggccg
     8101 tttgaagtcc aactcaatca ggctgaaggg cagatggctc gtgaccaggc gcagttgaag
     8161 aacgcccggc tggacttgca acggtacaaa gagctgctcg aacaaggctt tattcccaag
     8221 caacaactcg atacccagga agccatggtg cgtcagctgg aagggatcgt aaaagccgat
     8281 caaagccaga tcgataacgc caagctgcaa ctggtctaca gccatatcac ggccccgatc
     8341 agcgggcggg tgggactacg gctcgtggat gccggcaaca tggttcgcgc caacgacaac
     8401 aacggtctgt tggtcattac acaactcaca ccgattacga tcatcttcac gattccagaa
     8461 gacagtttgc cgggcgttct ggaacgcctt aaatcgggtc agcagctcac cgtcgaggcg
     8521 ttcgaccggg aacagaaaca cctcttggcc accggtacgt tactgacggt cgacaatcag
     8581 atcgattcaa atacaggcac ggttcggctg aaagcggtgt tcccaaacga agatggtgcg
     8641 ctgtttccga atcagttcgt caatgcgcgc ctgttgctgg acgtgaaaca cggcgtcacc
     8701 atcatcccgt ccgccgccgt ccagcgggga gcgaagggaa cgttcgtgta cgtggtgaaa
     8761 gacgacaaga cggtggccgt ccggccggtt acgatcggca cggctcatgg cgagaacacg
     8821 tcgatcgaca agggactgtc ccctgacgaa ctggtcgttg tggacggtac cgaaaagctc
     8881 cgtgaaggca gcaaggtcga ggcccggaat cagagcgaca agcccgcccc agagcctgag
     8941 acttcaccgg ctggatctga gaaaccgcga cgagggccac gaccgtgaat ccgtctcgcc
     9001 tgttcatctt gcggccggta gcgacctcgc tgtttatggt cgccattctg ctggctggcg
     9061 cgttggcgta tcggcagcta ccggtctcgg cccttcccca ggtcgattac cccacgatcc
     9121 aggtgctgac gttttatccc ggtgccagtc ccgacgtcat ggcctcctcg gtgaccgcgc
     9181 cgctcgagcg gcaattcgga cagatgcccg gactgaatca gatgacgtcc accagctccg
     9241 gcggcagttc ggttgtgacg ctgcaattca gtctcgatct gagcctggat gtcgccgagc
     9301 agcaagtgca ggcctccatc aatgcggcct ccacattcct cccgcgcgat ctgccgaacc
     9361 cgccggtcta caacaaggtc aatccggccg atgcaccgat cctgactctg gccctcacat
     9421 cgaatacgtt gccgcttccg caggtcgaag acctggccga gacgcggttc gcgcagaaaa
     9481 tctcccagct ctccggtgtc gggctggtca gcatcagcgg cggacagcgg ccggctgtgc
     9541 gcattcgggc aaatcctcgg gcgctgtcgg cctatggact caccctggag gacctgcgcg
     9601 cgaccgtcgc tgccgcgaac gtgaatcagg ccaagggcag cttcgatggt ccgcgacggg
     9661 cttccatcat caatgcgacg gatcagttgc tctcaagccg tgactatcaa cccctcatcg
     9721 tcgtttaccg caacggggct cccgtcgtct tatccgacgt ggccgaggtg gttgacgacg
     9781 tggaaaacgt ccgacaggcg gcctggatga acgaggtgcc ggctattctc gtcaacgtcc
     9841 aacggcagcc gggggcaaac gtcatcgaag tcgcggaccg catcaaaaaa ctcctgccac
     9901 aactccaaag cgcattaccc tcttcagttc aggtgacggt cctcaccgac cggacaacat
     9961 ccattcgagc ttcagtgcat gatgtgcagt tcgagctgat gttcgccgtc gcgctcgtga
    10021 tcatggtgat ttttctcttt cttcgaacgt tgtctgccac tgtcgttccg gcagcggcgg
    10081 tccccttgtc actcgtgggc acgtttgggg cgatgtatct catggggttc agcctgaaca
    10141 acctgacgtt gatggcgctc acgatctcga cgggatttgt cgtggacgac gcgatcgtca
    10201 tgatcgaaaa catctcacgg tatatcgaac ggggggagtc gccgctccag gctgcgctca
    10261 aaggatcgga acaaatcgcc ttcaccattc tctcgctgac catctcgctc attgcggtcc
    10321 tgattcccct cctcttcatg ggtgatgtcg taggccggct gttccgcgag ttcgccgtca
    10381 cgctgagcat cacgatcctc atttcggctg ttgtctcact tacgctgacg ccgatgatgt
    10441 gcgccaggct gctgcaccat cggcgacaat cggagcagag tacggtctat cgctggtctc
    10501 aacaactctt cgatcggacg atcgaggcct acggcaggac cttgaggtgg gtgctgaatc
    10561 atcaaccggc cacgctggcg gtcgccatcg ggacgcttgt gttcaccatt gcgttgtata
    10621 tcatcgtgcc caaggggttc tttcccgtgc aggatacggg ggcgatcctg ggcatttccg
    10681 aagcggccca gtcgatctcg ttccctgcga tggttgaacg ccaacaggcg ttggcccgca
    10741 cgatcctcgc tgatccggcc gtcgccagtc tgtcttcctt catcggtgtg gacgggacca
    10801 acaccacgct caacagcggg cgcatccaga tcaatttgaa accgatcgct gagcgtcgga
    10861 tgagtgtgca ggacgtcatt ctccgactcc aggggcagct ggccggcgtc gaaggcatca
    10921 cgctgtttct tcaaccgatg caggatctca cggttgaaga tcgcatcagc cgcacgcagt
    10981 atcaatatac gctggaagac gcggacccgg aggagctgag ccgatgggct cccacgtttg
    11041 ccgaggccct gcaggccctg cccgaactcc gcgacgtgag cagcgaccaa caggaccggg
    11101 ggctggcctc tctgctggtc atcgatcgga gtacggcctc gcggcttggc atcacacccc
    11161 agctgatcgt cgatactctc tacgacgcat tcggacagcg gcaggtgtcg accatgttca
    11221 cacagttgaa ccagtatcgc gtgatcctcg aagtcatccc tgagttccag aacggcccgc
    11281 aggcgctgca gtttctcgat attcggtcgt caacgggcgg tcaggttccg ctgaatgtgt
    11341 ttacccagtt ctccgagacg accactccgc tgacgatcag tcgccaagga cagttccctg
    11401 ccgtcacgct gtcattcaac ttggcgccgg ggacctctct cggcgacgcc gtcacggcga
    11461 ttgaacggac ggcgcgggct atcgacctgc cggccagcat tcgaggaagt tttcaaggca
    11521 gcgcacaggc cttccaagcc tccctgggga acgaaacgtg gctgattctt gccgcgctcg
    11581 tcacggtcta cattgtgctg ggtatcctgt acgagagcta tgtccaccca ctcaccattt
    11641 tatccacgct cccttctgcg ggcgtcggcg cattgctcgc gttgatgctg ttccaaaccg
    11701 aattcagtgt gatcgccctg atcggcatta ttcttctgat cgggatcgtg aagaaaaatg
    11761 ccatcatgat gatcgatttc gcgctcgatg cggagcgcaa agagggcaaa cagccgttgg
    11821 aagcaatcta tgaagcctgc ctcttgcgat tccggccgat catcatgacg accatggcgg
    11881 ccctccttgg agccctgccg ctggcgatcg gctcaggcgt cggttcagaa ctgcggcacc
    11941 cgctcggcat cgccatcatc ggcgggctta tcctgagtca ggcgttgacc ctatacacca
    12001 caccggtcgt ctatttggct ttcgatcgat tggcgcgccg tggccgttcc cgtcggtcct
    12061 ccgcacccac caccgaggcc ttgactcctg gtacggtatg aacatctcgg caccgttcat
    12121 ccaacgtccg gtcgcgacga ccctgctgac cttcggcgtc acgctcgtcg gcatcgtggc
    12181 attccggttc ctccctgtcg cggctctccc tcaagtggag tttcccacaa tcagcgtgtc
    12241 agccaatctg cccggtgcga gccccgaaat aatggcaacc tcggtggcgg gtccgctgga
    12301 acatcagttc acccgcatcg ccggtgtgac ggaaatgacc tccaccagca tgctcggctc
    12361 caccagcata acgctgcagt tcgaattgaa ccgcgatatc gacggtgccg cgcgggatgt
    12421 ccaagctgcg atcatggcgg cgcgcggcga tctgccggcg aatcttccgt acagacccac
    12481 gtatcgcaag atcaatccgg ccgatgcacc gattctcatc ctctcgctga cctcggatat
    12541 cgtgagccgg ggccgaatgt acgacgtcgc ctccagcatc cttcagcaga aactgtcgca
    12601 gatcgacggc gtggggcagg tttatgtggg agggggctcg cttcctgccg tccgtgtcga
    12661 tctgaatccc acggcgctga ataagtacgg gatcggactg ggagacgtcc gccgtatgct
    12721 gagcagtacg aacgtgaatc gtccgaaggg ccagctcgcc gatgggacgc gcacgtggga
    12781 gatccggacg aacgatcaac tccacgatgc ggaggattac ctcccgttga tcgtgagcta
    12841 ccgccagggg cgggctgtcc gcctgtcgga cgtcgcgacc gtggaacatt cggtcgagga
    12901 tctacggacg atgggtgtgg cgaacggggc gccagcggtc ctcatcatta tcagccggca
    12961 gccgggagcg aatatcatcg agaccgtcga ccgtgtgagg gcaatactcc ctcaattaga
    13021 ggcctccatt cccggcagca tgaccctctc gatcgtgggc gaccgcaccc ccgtgatccg
    13081 cgcatccctg cacgacgtgg aacgaacgct ggccatctcc gtgctcctcg tgatcctggt
    13141 ggtcttcctg tttcttcggg atgtccgcgc gacccttatt ccctgtgtgg cggtgccggt
    13201 ctcgctgatc agtacgttcg gcgtgatgta cctcttgggc tacagcctcg ataacctttc
    13261 gctgatggcg ctgacaattg cgaccggctt cgtggtggac gatgcgatcg tcgttctaga
    13321 aaacatcagc cggtatcgtg aacagggtat ggctccgatg caggctgcac tccgcggggc
    13381 gcgggaaatt acgtttaccg tcttatcgat gactttatcg ctggttgcgg tctttctccc
    13441 aatcctcttc atgggcggca tgatgggcag gctgttccgg gaattcgccg tcaccctctc
    13501 cgtggcgatt ctggtttcgc tcgtcgtgtc cctcaccacg acgcccatga tgtgcgccag
    13561 ggtactgagg tccgaccagg gccggtcgca cggatggtca taccggctca tcgcacggct
    13621 cttcgaaagc atgcgcggcg gatacgctcg gagtctgacg tgggtattgc accatcctcg
    13681 cggcatgctc gccctcacgc tggtgacgat ggccgtgagt atctatctct acaccgtcgt
    13741 ccccaaaggc ttctttcccc agcaggatac cgggcgcatg ttcggaaaca ttcaggccgc
    13801 tcaggacatt tcgtttcaag ccatgcgtca aaagctcaca gaggtggtgg acatcatcaa
    13861 aagcgatcca gcggtcgaga cggttacagg gttcagcggc ggtggcggca acacgaactc
    13921 cgcccgtatg ttcattacgc tgaagccgct tcaggaacgg cagatcagtg tggatcaggt
    13981 gatcgcgcgg ctacgcccta agctggctaa ggtgccgggc gcgccgaccg ttctgcaggc
    14041 gattcaagat ctccgcatcg gcggtcgcgc aagcagcgcc cagtaccagt acacgcttca
    14101 aggtgtggac ctggccgagc tgaatatgtg ggcccctcga gtggaacgcc agttacgcac
    14161 cttgccggaa attgccgatg tgaatagcga ccagcaggac aaagggctgc agtctttggt
    14221 ggtctttgat cgcagcacgg cctcgcggct cggtctcagt ccgcagctca ttgacgacac
    14281 cttgtatgat gcgttcggcc aacgccaagt ttctatcttg tacacgcccc tcaatcagta
    14341 tcacgtcgta atggaagtgg ccccccaata ctggcaaaac ccctcgacgt tgcaggagat
    14401 tcatgtgcgg tcgtcgacgg gagcgcaagt tccgctgagc gcggtgacac gctttgaacc
    14461 gacgaacacc ctgctgatgg ttaatcacca gggacaattt cctggtgtga cgctctcctt
    14521 caatatggcg ccggggactt cgctgggcga agcggtgcag gcgatcgaaa aatcgatgca
    14581 tgagatcgga ctgccggcca gcatccgggg aaattttcag ggtgcggcca aagcctttca
    14641 ggcgtccatc gagaaccagc ccttgctcat cctcgccgcg cttatcacgg tctacatcgt
    14701 gttggggatt ctctacgaga gctatgtcca tccgttgacg attctctcga ccctcccctc
    14761 tgccggcgtc ggcgccttgc tcgcactgct cctctttaag acggaactga gcatgatcgc
    14821 actgatcgga attattctcc tgatcgggat cgtgaaaaag aacgccatca tgatgatcga
    14881 tttcgccttg gacacggagc ggaagaatgg caaacggccg gaggaagcga tctacgaggc
    14941 ctgcctgtta cgcttcaggc cgatcatgat gacgacaatg gcagccttgt tcggcgcact
    15001 gccattggct ctggggacag gcgtgggatc ggagttacga cgaccgcttg gcattgccat
    15061 tgttggtggt ttgcttgtca gccagttgct taccctctat acgacgcccg tcgtgtacct
    15121 ctacctcgat cgtctgagac tgcgcttctt gcggataaca aaagggctgc gggagggaac
    15181 caaacagcca cctccgcctg gcactcacca gccaggctac tcataagagg cccaagggca
    15241 gtcggttgtt gcaatcttgt ctcaacctcc gcttgattct ctctaagcgg agtgagcggc
    15301 ccgccgtgct tgtacgaggt cgaatatcac ttacccgcat tgtgatctgc tccgccaata
    15361 caggttaaac aagatctgag tggtctttat ctacgggaca ctctttgttt ccatgaacgc
    15421 cgtgatcgcc tccatagtgg ccgtcggatt cgtatagatc gcgaagtggc ggcccttgat
    15481 cctgcgcaca gacacggacg gtcgtacacg gacgatctcc tcaacattgc gccaggggac
    15541 gacaccgtca ttgctccctg cgatgcagag caccggagcc ggacacctcc tcaagagttc
    15601 tcgcgcatcg atgttcagca acgaccgaat gcgtgctgct actatgtggg cggtcacacg
    15661 tttccagatc tccgccgtat ccaggcgaag ctgatcggtc ggccctctgg aaagccaggc
    15721 gggaatgtga cgacaggtgc gaagcaacca gatagtcggc gcaactgagg tgaaccgcat
    15781 tcgcacatag atcctgcgcg gcggactcac aaacgtcgtg gccaggatca cgccctgaac
    15841 cttgtccggt tctgcctccg cgagcatcaa cgctagcggc ccagcaaacg atgatcccag
    15901 cacatagaag cttgggatcg ctgataccgc gtctcgcaca atcgcgagga ggtccgcata
    15961 ctcagtggcg cctgctggtg gaaaggagac cacgtgcggt ctgacccact ctggaaacga
    16021 cgccagcagc ggtcggaaaa agacctccgt cccgtccagg ccgggaagca gcaccagcgt
    16081 ctccagcttc gcgtttccca catcaagcag caacgagaat cctccctgtc attatccagt
    16141 cgatctgact tgagcacaag gtcaccaaac ggacattaca cagcctttat ggcagctgaa
    16201 ccacagtaat tgtccattcc ccagtcccgc tcacttgaag ataatattct ccacctttgg
    16261 gctgatagga tgatccgctt cccggtcctt cctgcgttgc ggcgacggtt gataatttac
    16321 catcggtggt gaagattgtg attgtcaaca gtgtgccctt ggcatcccac tgtatttccc
    16381 acttatcttt cgccgtgaac ggacgcagat ttctctgccc attcgctgag actttttgga
    16441 atacttgttc gctttccgcc ataataagaa tgggagtagc aacgtagaga aaacaagcga
    16501 tcaaacagag ggataggcat ttcttgagca gcatgttgat tctcccttct cattatttct
    16561 gcttcgccac aatctgctcc ttgatcttgt cgtaggtctc ttcctcggtg gatatggttt
    16621 cacgttcggg ggcgttggcc gttcgtgtat tgcgcgatca cttcatgctg caagcaactt
    16681 cccacaagtt tctatcaaga ctatctggtg ccactggttc ccacttggtt tcgtctaagt
    16741 tgctatacac cgcttcaccg cttcccatat tccctgagaa agtcgtgaac ccaagcagcc
    16801 ggacgcgctc ttttacgcag tcatattccc ttcgcgcttt gctagataac cacggcccac
    16861 ccactatgct ttgtattgtc ttgtagtcga acaatgccca caacgtcacc acgtccccgt
    16921 tgcggcgaat ggtgtctgga tcgacgtata ccgtgtagac agtcaatcct tcttcgactt
    16981 tgccaccaac atccacccac tccgcatcca ctggttctac actcaataac aggattgtta
    17041 tcagtgacca aacacctgaa cagtggaaca gcggcagaaa gcgacggtag gattgaatcg
    17101 tgaaggccat gagagcctcg aaggatggct caattctagg agtgcggtaa cactggcgca
    17161 agggccgagt ggggaaatgc gtatattcct gggctgacac agttttgagg agagtccttc
    17221 caggtcatcc gtgcagaagt gcgaaggatc agggcaggga ggtagctcac agcttctcta
    17281 cttctccgac gatggtccac cccgttttaa gaaaagcatg acagagcgac tcttacgtgt
    17341 taactctaac agggaatgcg ccgtctctag aacacggtag tattcatttg atccttggag
    17401 atgacgatga tgaccagaca gacacaaggg gctatcacag accgtcatga taaggcggtg
    17461 gtgcaagatc gtgggggacg ccgggcggaa ttgtcgtgcc gcctcttctt ctttggcgac
    17521 gatgactttg aaggcgaagg caccctgctg gatatttcga cgaggggttg ccgcgcctca
    17581 tccctgattg cagtgcagcc cggaatgctc ttgaagttgt ccgtgttcct ccccgattac
    17641 ttatggccct tacgagtcga cgaggcgatg gtccgatcgg tgaacggtca ggaattcgcc
    17701 gtggagttca ccagtattcg actggcccag cgggaacggc tccgcgctct tgtgatgaag
    17761 gagaagccat aacgcacctc ctaatgatgg gagacatgac gaccataagg agaccatgtc
    17821 gaagattctc gtgatcgatg acgaggaatc cgttcgcatg ctgcttcgtg ccgtgctgga
    17881 agaggacggc catgaggtga gggaggctac ggatggacga accgggatcg ccttgtaccg
    17941 tcaggagcga gcagacttgg tgattgctga catcctcatg cctgacctca acggcctcga
    18001 catgatcgtg gagctgacgc gcgagtttct cgatgtgaag gtcattgcga tcacaggagt
    18061 atacggagaa ccagacctgc tcaaacgggc cagattgttg ggcgcgcgcc acaccttcta
    18121 taagcccttc agtcctgagg aactcctcaa agtcgtgcgt tatgagttga cgcactgacg
    18181 acctatgtct gaggagaaat accccaaatt gtcggtgact ctgatttggc gacctgccgt
    18241 ttacgagatc ccgtaagtac tgccaccagc aacatatccc cacgggaaac gagagaacag
    18301 gactccggca cagttggtgg tgatggggct ggtttgtcct gtcctggtag cagcgtggta
    18361 gcaaaaaaga tgattgaagg agaggggcca atagatgctc tgcctgggat agaaaaaggc
    18421 gcttgagggt aaaagtgacg gagttgctgg gctaactaaa atttccgttg agcctttttc
    18481 tcacggtcga aatggccttg agtatggact tctcgagttg gtctccgctt accgtcgtct
    18541 tgtgaaatgc agcttgtgcg ccacttttta tggcgagttc taacaaaaat ggattgggta
    18601 accttgtaag tacgatcacg gcgatctcgg gatgagaggc gcgcggaact aacgttgaca
    18661 aaaccgcaaa gccagataca tccggcaaat caatttcaag cacgacacaa tcaattggtt
    18721 gccttgcaca gatgtctagt ccagcccgac cagtcgccgc gaaaactata tggtaatttg
    18781 atgaggatcc ttggagctgg cctacgcaaa attcatattg tcgggcatcg tcgtcgacga
    18841 aaaggatggt aatgccgtcc gacatcgcaa ccgcctctcc ggggaaatct tccctgcgtc
    18901 cattccgcgc tcaggttgga gcagaccgac agtggatatt tcacgggagc ctatatttat
    18961 gccttgtgcg ggagtgtggt tatgaattcg aaccatcctc cttatcgaga ctcctcgtcc
    19021 ggattcttaa tcttctcatc atatttgtta tacgagttcc cgcactttgt cggacaggac
    19081 ggtcatcgcg atcttcactc gattcggttc tccccgcgcc aaagccttcc agaagtggac
    19141 cgcttgatca agcgtcactt tggggggcat cggcggttca aatggatcca cgattgcctc
    19201 gacaatgacc ggccccggtg tggataaggc ttgggcgagt actcggccgc agtcggccgg
    19261 atcatcgatc ctcagtccag ttcctccaca ggcctgcgca aagagcgtga aatcgatcgg
    19321 ctgcagatca catccaaatt ccggattccc tagaaagacc atttgctccc atttgatcat
    19381 gcccagggag ttattcttga tgatcaccac cttgaccggc agcttatact tgacgcaagt
    19441 cgcgaattcc gccatcagca tggaaaagcc accgtctccg acgaaggcga tgcactgtct
    19501 atcgggatac gcaatttgcg cggcgatggc gtacggcaac gcattggcca tagaggcaag
    19561 attccccgaa agcgagtgca tctggccacg ctttgccggg atatgcctcg cccaccatgt
    19621 cgcgatcgtg ccggagtctg ccgacacgat ggcgttggcc gaaagctttt tgccgagctc
    19681 ccaggccacg acttgaggct tcatcggttt gtccggcctg gtaccgatct ccttcatcat
    19741 ctcccgccat gatgccattt cgttctgcgc ttgctgcaag aaatcgcggt tttgctttgg
    19801 ctgtaacaag gggtgcagca actgcagcgt tcggcggcaa tctccgacaa ggccgacctc
    19861 gatcggatag cgaagaccga tgcgggccgg gttcaggtcg atctgtaccg ctcgtgcttg
    19921 gttcggcttg ggcaaaaact cgatataggg aaacgaactc ccgaccatca acaaagtatc
    19981 gcattgctcg aggacattct gggaaggctt ggtaccgagc agtccaacac cgccagtcgt
    20041 atatggactg tcatcgggaa ccgcggcctt gcccaagaga gctttgacaa tcggcgcacc
    20101 gagaatctcc gctgtttctt ctagctcatc tgtggcattc aacgcacctc ggccagccag
    20161 aatggcgact ctctggccag cattgagcaa atccgccgcg cgtgagaggt cctgtgaatc
    20221 cggcagtcga gcacttcgtg caaaatgctc ggacgtatga tgaggaacgt tgcgttttga
    20281 gccatcccgt tgagcatcct gttcctgtac atccacgggg aatgtgatgt gggccactcc
    20341 gcgataactg agggcggtgc ggcaggccag atccgtgacg ctctcaacat gtgtcgcgcc
    20401 cattattcga acgttgtaca cgcacacgtc gaggaacagt ttgtccaatt cgacatcctg
    20461 ctgcgcgtgc gtcccaatca ggtcgtggaa atggagcccg gtaagtgcaa ggaccggctg
    20521 cccatcgagc ttggcatcat agagtccgtt caacaagtga atgccgccgg gccccgatgt
    20581 cgcaagacag actccaagtc gcccggtgta ttttgcataa gcacacgcca tgaaggcagc
    20641 cgactcctcg tggcgaactt gaacaaatcg tatccggtcc cgccgcgtac ggagcgcttc
    20701 gatgatgccg ttgatgccgt cgccgggaag cccgaagatc accgagacat cccaatcaat
    20761 caatcggtca atcaagactt cggcggctgt agtcatagga ggctgccttt cttacttgtg
    20821 aagaagacct tggcactggt ccatcagtat gtaatgtccg tcctgcccat ctcgggatga
    20881 gatgagatgt tcctgcggca ttcgtgcatt tgtccgaatc agcccttgtg catcttccat
    20941 tggaattccc tcttctgctc ctcgctttgg catgtctttt tgagttccct caatgcagag
    21001 aagaacttgc taatgccaag acagattgtc ttgtggagaa caagttgcac ccctgctgat
    21061 tgctataaag acagaataag caagtctgaa tcatttccta atctccaaac atctcaaaaa
    21121 cttgtgcgtt catcgcatct ctggatggat ggataagatg tggtctatca cttgcaaaaa
    21181 ccatgacaag gcttcagaaa ggccagaatt ctacgacatt cttgcaagac cgcatcggta
    21241 tatgccttgc acatttttct gaagtaagga ggcgaacaat gatacctaac ccgaactaca
    21301 cggcatgtaa agcgctcact ctcctaatcc cgatgctcgc gatcattgcc ggatgcagcc
    21361 atacgattcc accggcaaac acgagaccac agccgccgcc tcaattgggc aagacacccc
    21421 cggtcattga gccgctcagc gcaagtcgca agcagctgca agccgccgaa tctgtactgc
    21481 cggtgacact cacggtcgac ctcagcccgg tgcagcgcgc cattcaagcg gcggtgcctg
    21541 accaatttgt cgaaacgaat cacccgctcg cagcgaacta tcgctggaga ttcgttcgag
    21601 agggtgaacc gaaggtccaa attcaagacg gcctggtgaa attccaggca ttctaccgtg
    21661 gggagatcga atcgaccgcc gcgcgagcct gtcggctcga tcccctctat ccaatactcg
    21721 aggggacggg acgactgatg ttgcgggaac aggaccaaca actcctcgtc acgatgaccg
    21781 atatccagac gacggtgagc ttaaagccag agagcgacag caagtgcaat atgttcaacc
    21841 ttcctgtgaa agatcagatg gccgagttga tcaagcagga atcgcttaaa cagcagatcg
    21901 cccaatctat cgaacaggca ggatatacga taccgattca tctcgtatgg gagcggttaa
    21961 atgagcccat gccgatgacc gtggcgtcgg cgaacacaca actctgtctg tacggcaaag
    22021 tgaaggaatt taccgtcggt tcgctgaaag gcccggcaca gcagacgacg atctcggggt
    22081 tggccaggga aacacctgtc gccatctatc agtcaccctg caagcagccg tccgtgacac
    22141 cggtcaaggt gcaggtgggt tcgacggcag ccgcgatggc cgaaggacag ccatacaatg
    22201 ttctgctcag tgtgccgatt ccctatgctg tgctgaatca ccaattgcag gaccgcctct
    22261 ttcatcacga ggtcaggctc gataccacct ttaaggacaa gatcttggtg gagcaggtag
    22321 tcgtctccga cgcgaatggc cgggtcctct ttgcggtaaa caccagcggg gatttaaacg
    22381 gcacgttgta ctattggggc acaccgcagc tgatcgacga agggaaccgc atcgttatat
    22441 ctgatctcca gatggcgaac gaaaccaagg tcgcactaga cgacatcaaa gtcggctact
    22501 ggcaattggt ggatgaccaa ttacgagata gactgcagcg ggccgccacc ctggatttgt
    22561 cccaacgctt cggaaacatg aagacagcga tgagcggcca gcgtcaggcc gggggattga
    22621 ccacggatgt cttgatggga cgtcaggaag cacaacgcgt ctattccacg gcggacgccc
    22681 tcgttgtcga cattttgctg cagggaaccg cgagcgcggc aggacgggtg ccggtagaac
    22741 agcatgctcg gatggtgcgc atcgagccca tgccgttgga tcgggatcct gttgcaacga
    22801 caccgtcgcc gtcagcaccg ataccagcgg accgacctta gaagcatcgc ctaccgtgcc
    22861 gattctgctt tgcctcacga cggtggaatc ggcacgcctc cgtggttcgc ctcgtacaaa
    22921 ctgacgcgaa gcctttgact tcttgcggta gacgttcaac ccgacttttt cctcgcggtt
    22981 ggggatcgtc acattcccct cggtcgaagc gacgacggtg gtcttccccg aggccctcgc
    23041 cttaaaagtg tggagggaca gttttactag cggcttgatt ttacgaaggt ttccccgttt
    23101 tatccgtaca atcagtagtt tcagacgttt ctgttgattc aattgtttct attagatgcc
    23161 atgcgttctg cacagtttta gcacaaattt ggcactccaa cgtcttattg gccgagacat
    23221 cgaaaccata ataggctatc ctagcctccc cggaaggctt ggatgagtga ttcatgacaa
    23281 atgaagaacg tatccaggtc tttgattctc aaggccaggc ctacaaacag gcctttcaaa
    23341 ctttcctaga ccataccgac caaaagcgta atgccaaacg ctggctgcag cagattgtcg
    23401 atggtctttc cacgcggaag gtattcattg acgccggcgc gggcaatggc gaggtcactc
    23461 gggcattcgc ggacgacttt gaacgcacta tcgccatcga accgaatatc tttttggtga
    23521 agcagctgca ggaggcggtg ccgacggccg aagtggaagt gatcgatgag cccattatgg
    23581 ctgctcaacc cactgctcgg ggagatttgg tactgtgctc acacacgctg tactacattc
    23641 cgcccgaaga gtggctctgt catctcgaac gcctggtgtc gtggatgtca cccgacggcg
    23701 tcaccatcgt tgtcttgcag caccgtgaga ccggatgcat gaacatgctt catcactttt
    23761 acggacagcg gtttgaaata cgccggaccg ccgaagcgtt tcgcgtgaag catggagagc
    23821 tgtacgacgt catgacgacc ctcgatcctg cgcacgtggt gaccccggac ttagagaaga
    23881 cctacgctgt cgccgaattc atgctcaatc tgttagaggt caaatgcgca ccggcccgcc
    23941 gagaggtgga agcctatgtg caggctcact tcgccacagg ccgcggcggc tactgcattc
    24001 ccgtgcatca agactttttg cagattcgcc cccttcgccg cggagagatc atcacgtgac
    24061 gtcgcgttca aaggtttttg taatgagtgc catggcttta gagaatggca cgtcgggaag
    24121 tgctgaattg gctggaacat ataagtggtt tatttttttg tgacgcgcgg cagacgtatt
    24181 gaaagccgct ataccgctcg atccggataa ttgggaagcc tatatcactt aggatatgtg
    24241 tacggggata tgggacagca tgaagaagcg attaggcatt aaagcgacga actggatcgt
    24301 tcgaaatcaa aataatgatc gttaaacacc aacaggacat cgcttcgcga gatagtcaga
    24361 aacgcaacga agccacaaag gtttactcct cactcattcg ctaatctgac ttgcgtgcat
    24421 tttgatttca atcggcggga acaggcaggg tgagcgggtt tgcgggtgcg atgaatagct
    24481 tcctgtgacg gtcgatacta tgcgttgaga ggcgatgcct tggccgcctc agtcatgaga
    24541 tgcaataagg attggcatct ctcctgaatc cctgtccaaa acaagttgtt aagtctggct
    24601 ttggcgttcg tactccattt gacaaacttt acgccgaagt gtgttccgat agcccactgc
    24661 actgtggcat cctcaatcat gacgggctct tggtcgttag agagatagag gactagcgcc
    24721 agtcgcatcc cgataggaac cggttcctgg ctttcgattt gaagcccttc tcgtgagaca
    24781 tcgatcacca gtcccttcgc cattatctca ggggtgagat agttgatcga agcctggaat
    24841 gggatgcggg gcacgcatcg atccgtccat gctttctcgt ttatggccat attttcacct
    24901 gtgctggagg caattaccga ttgaccatcg gtattgtcca aatatgctcc aatggtgacg
    24961 gtctgtcacg tgttacagca taaattgcag tcaaatctca ttggacgtcg agtgggcaat
    25021 aggccgcgac tttcattacg ctccaggcgc ggaagccagc agcaaagtgt ttgagaacag
    25081 acagggaacc tatgacccgc tgattaagag ggagaaagag ccattttgga taaggctcgc
    25141 tattgcaacg gttcccccaa attgattgca atctcgttag cttgaagcgt tccattagtt
    25201 ctatcctttc ccgtcctttc ctttcccttc ccgcctagtc tggtcacaaa agtggtcaca
    25261 cagcgcgacc cgccgtgaag gtgttgtcgc gttccttgta gtccgtcgtc accggcgttc
    25321 agtcttccac gcccacgccg atcacgatga gaaccgcata gtcctgcttc gtgcagccct
    25381 cgaccggagg catcgctccc cctggcgcaa ctggaaacgc agtcttctgc gcgtttgccg
    25441 gcgagcacga accaccaacc gctgccggtg tatagcgcgc ggagcggcag cccaggtgcg
    25501 tcacgtcgta gagcgtcgcg cctttatcga gctcccagcg cctatcacca ttcctgtcgg
    25561 cgggatgaat ggtcagcaca gccgtcacct cgcggttacc cgtgaccacg tatttgccgg
    25621 gagggagcgg aaaggtcggc tgctccatga tcaagcggtg ttcctccagc caccagtccg
    25681 tgccgtcgaa cttccagcgc gccggggcga cgccaccggc atccttcaac cgtgtatagc
    25741 tgttcagctc gacgccccga ggacctgggt ccagaggcca cagaccccac gattgcgcgc
    25801 cgctcccgga ggtcgcacct ggatcgccca acgccgcgat gaactgtgtc gatatgcgct
    25861 ggaacttcgt ctgacctccc cctgcggcgt gggcttggct tcgactcaga ttgagt
//
LOCUS       DATIFR010000052        25145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2139584, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000052 DATIFR010000000
VERSION     DATIFR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139584"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VLD60_02945"
     CDS             <1..291
                     /locus_tag="VLD60_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB43937.1"
                     /translation="QRVSIARVLLKDPPIVMFDEATSNIDTETEVKIREALDELTRGR
                     TTIVIAHRLSTVHGMDRIIVLEQGRVVEDGPHDVLIARGGVYAGLYEAQFQT"
     gene            304..498
                     /locus_tag="VLD60_02950"
     CDS             304..498
                     /locus_tag="VLD60_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43938.1"
                     /translation="MVLIYENDPLDNEHARGRFYHVCRGQVDRTELQIPPADQPHECA
                     RCGIDLEAEDFFVAMQKGSV"
     gene            503..712
                     /locus_tag="VLD60_02955"
     CDS             503..712
                     /locus_tag="VLD60_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43939.1"
                     /translation="MAGSSGRKTVSVMRGLMMLCESCYDQVFLEKLVDHKNYVADHEA
                     LFDTICPGCTDINRPLIDDMLGSSE"
     gene            complement(740..1066)
                     /locus_tag="VLD60_02960"
     CDS             complement(740..1066)
                     /locus_tag="VLD60_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43940.1"
                     /translation="MPFSRTLLLSVFFTLLLANSSSAEDKSFYSPVINIDKDAHRILI
                     STLGSVFWIDVPEAAQPHMEKLPLSGLVDFVVEMRGSGQAPLLKTWKVKSGESTCMYF
                     DGKVCK"
     gene            complement(1168..1680)
                     /locus_tag="VLD60_02965"
     CDS             complement(1168..1680)
                     /locus_tag="VLD60_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HSB43941.1"
                     /translation="MSDLIIPQQILDDLVAHARELDPYECCGFLAGKDHTVSHIYRIK
                     NIVSLDGAAQVASFDDAKVQHLSRLSPEERAEIAFIMDAQEMFQAVKDMRKLGLTLQV
                     VYHSHPHDPARPSVTDIKIANEWEENWSRLNLTLPIYLLISLMDKSNPDIRGYRIKGG
                     TVAPAQLTTP"
     gene            complement(1683..2486)
                     /gene="moeB"
                     /locus_tag="VLD60_02970"
     CDS             complement(1683..2486)
                     /gene="moeB"
                     /locus_tag="VLD60_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HSB43942.1"
                     /translation="MELTEQQIQRYSRHIILQDVGGKGQLKLSKAKVLLIGAGGLGSP
                     AALYLAAAGVGTIGLVDGDVVDLSNLQRQILHSTATVGQPKVESGRKTLHAVNPEITV
                     NAYHQLVDAENILSLVRDYDVVLDGSDNFTTRFLVNDACFFAKKTLVSASMFRFEGQL
                     TTIKPHAGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLLIYDALDMKFRKVGRPKDPACRLCGPNPTIKDLNSDYTVSCTI"
     gene            complement(2490..2729)
                     /locus_tag="VLD60_02975"
     CDS             complement(2490..2729)
                     /locus_tag="VLD60_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HSB43943.1"
                     /translation="MSHLRFHVRFPEDKVKEPIIYQIGREYNIVTNVRRADVRETTGW
                     VDVEISGETAEIERAIEGLRKKGCVVDPIELNVVE"
     gene            complement(2732..3004)
                     /locus_tag="VLD60_02980"
     CDS             complement(2732..3004)
                     /locus_tag="VLD60_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSB43944.1"
                     /translation="MIKIRIPTPLRPLTKGQGEVEAQASSIANMIETLNQSHPGLKDR
                     LCDESGELRRFVNIYVNEEDIRFLQGKETSLKDGDEVSIVPAIAGG"
     gene            complement(3001..4251)
                     /gene="thrC"
                     /locus_tag="VLD60_02985"
     CDS             complement(3001..4251)
                     /gene="thrC"
                     /locus_tag="VLD60_02985"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSB43945.1"
                     /translation="MAKMKALVCRECGKEYPTKAIHVCEMCFGPLEVKYNYDEIKRSI
                     SRKKIGEGPQSIWRYADLLPVEGPNYLGPHAGMTPLVRAKNLGAYLGLDELYIKNDTV
                     NHPTLSFKDRVVAVAITRARELGFETVACASTGNLANSVAAHAAAAGMRCYVFIPGDL
                     EAAKVLGNLIYKPNVVEIEGHYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWRTPDQAVIPMASGSLLTKIWKGLHEMKAVGLIDEVRTKINGAQAEGCSPI
                     STAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATAESNGSMDMVTDDEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLQKLVKNGTIKKGDVTVAYITGNGLKTQEAVIDAVGRP
                     VRIQPSLVAFEKTFKMGKNGGGDA"
     gene            complement(4280..5092)
                     /gene="moeB"
                     /locus_tag="VLD60_02990"
     CDS             complement(4280..5092)
                     /gene="moeB"
                     /locus_tag="VLD60_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HSB43946.1"
                     /translation="MEFTEEQISRYSRHILLPEVGGKGQKKIAKAKILLVGAGGLGSP
                     AALYLAAAGVGRIGLIDSDVVDLTNLQRQILHHTPDIGRPKVLSGKEKIQALNPDVAV
                     SMYEERLTAGNAIKIINDYDVVIDGVDNFTAKFLINDACFFADKPLIHGGILRFDGRV
                     TTIIPRKSACYRCVFKTPPPPGLVASCQEAGVIGVLAGIIGTIQATEALKLVLGIGRP
                     LTDRLLDFDARKTQFREIKVKRNPNCALCGEHPTITELFDDGDPFAGCAVRP"
     gene            complement(5098..6027)
                     /gene="cysK"
                     /locus_tag="VLD60_02995"
     CDS             complement(5098..6027)
                     /gene="cysK"
                     /locus_tag="VLD60_02995"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HSB43947.1"
                     /translation="MTMILHKEITELIGRTPLVRLNRLSKPGCATIYGKVEFFNPGGS
                     VKDRICLNMINEAERQGKLKPGGTIVEPTSGNTGIGLALIAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTPAWEGMKGSIREAESLLAQNPSYFMPDQFSNPANPAMHRMTT
                     APEILDALDGKIDAFVAAVGTGGTITGCGEVFKERNPNVKVVAVEPAGSPVLSGGDPG
                     PHKIQGIGAGFIPKVLNRKIIDKILTVTDDEAYQTAKQLSKQEGLLVGISAGANVFAA
                     QKIAEELGPGKNVVTILCDTGERYISIEKYFNI"
     gene            complement(6024..6224)
                     /gene="thiS"
                     /locus_tag="VLD60_03000"
     CDS             complement(6024..6224)
                     /gene="thiS"
                     /locus_tag="VLD60_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSB43948.1"
                     /translation="MQVKINGKAEEFPGGTVLDLLKTKKIEPQMVAVEVNDAMVDREH
                     LGTIHLNDGDRLEFLFYMGGGR"
     gene            6378..6755
                     /locus_tag="VLD60_03005"
     CDS             6378..6755
                     /locus_tag="VLD60_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43949.1"
                     /translation="MWKRNFMFRASDATPLKESENELFHETDPAMDSVGLQLEKFLSV
                     WIQGEGDDEKPSAFTNMYVRTATLDFQKRVGFLQPLQGRTHQIKQLLTPGQKEFLRNW
                     LSTNAPQAWDSTDEHFKMLFELE"
     gene            6752..7324
                     /locus_tag="VLD60_03010"
     CDS             6752..7324
                     /locus_tag="VLD60_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSB43950.1"
                     /translation="MSHRDASTSRFPRRSRFLFCGLLCSLLLAQEPKAWGAGVEVYYS
                     PEDAPLDRVVSLYEHAKRYIYVSVYGLTSPRAVEALVAAKKRGLDVRFITDQERAQEV
                     KQRTALHTLRLAGIPIQVNQHDGLMHLKQVVIDDEVNTSGSMNHTTSGNRYNDERMDV
                     ITDHAITVKAREKFLAMWNDHERYRAWTDD"
     gene            7330..8613
                     /locus_tag="VLD60_03015"
     CDS             7330..8613
                     /locus_tag="VLD60_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSB43951.1"
                     /translation="MTERLEQTDVVVVGAGGGGAVLALALAQKGINTIVLDHAQGPPQ
                     GLRGEILQPNGQSVLDRLGVLQSLPTEATRSVRNFHFCRAGGKRLCSIDYGDLPAPYN
                     RAIVTLPNVAHHAIVDAVQQQPSASLRYGTSFAGLLREGGRVVGVTTQGSEGTRTIRA
                     KLVVGADGAFSKVREALKIPADVYFYPEGYLIAIVESEEPVSESFYYVGHRQILGLFP
                     ATGNKVYLFYMIPSGSYEAVTQRGIASLQQAWSNIAPQFAKLFQNLTDWSQTAYMPTG
                     RVRTPTWVADGAVLIGDAAHAMNPHASQGRMQAMVDAMALADLLPACLADNDCSAARL
                     KPYETVRRPHVTMLQRLADQQVFYWNTGNPVVAYLRDRVFQTLDRNPRLRYQVLSTTA
                     GLRPTAPFNLIDRVIAAGLLPDLRASGRASHPMSS"
     gene            8620..9384
                     /locus_tag="VLD60_03020"
     CDS             8620..9384
                     /locus_tag="VLD60_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43952.1"
                     /translation="MSTTERVIEGLPEETQNLLYAYVKDVKGLIGEQLEGVLLYGSAV
                     RGEFLQGRSNLNLLLFVSSYDQALLKGYSAIHRRWSKEQIVAPLFLTEEELRSSASVF
                     PLEYLEIQEQHRVLGGRDPFVGFRVDTQRLAAEVRQGVVGALLRLRQRYVEGNATDEA
                     ALILLPLSVTALLPLLRGVQRLQGRAVTYQSDAVVKDVAELLKLDLQGFHDALLLKRG
                     QITPGPKEIPRLFGRYLQALTALANRVTGLVPDGDR"
     gene            9381..10100
                     /locus_tag="VLD60_03025"
     CDS             9381..10100
                     /locus_tag="VLD60_03025"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSB43953.1"
                     /translation="MKIWLALLILVLFGGHAEAVPYDRPKIQLPDPRGYVSDYAQVLD
                     GDWKERIRSVCQDLERKTGVEMVVVTVPSIKPFGSANEFATAIYEKWGIGSTQQEHGV
                     LVLVAVQERQAAVTLGRQMLPIITPAVMNQVGGEYLHPSINLGHFGEGLYRTVVVLAS
                     RSQEVRVGSLSKTHFKGVGFWITLFTSIGALSFLWWISRPDMRHPYSRIRKGEYWGTG
                     QGGFGGNWGGFGGGTSGEGFR"
     gene            10350..12611
                     /locus_tag="VLD60_03030"
     CDS             10350..12611
                     /locus_tag="VLD60_03030"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSB43954.1"
                     /translation="MAIVWSVTIFLSAFLLFLVQPMMAKMILPMLGGTPAVWNTCMLF
                     YQATLLAGYGYVHLLNARVAPRRQLPVHLALLALPLLLLPIGLPAGWTPPDESNPVFW
                     VLLLLTVAIGLPFFVLSTTAPLLQKWFSWTDHPSAGDPYFLYAASNAGSMLALLGYPL
                     LIEPWLPLKGSERLAQTTFWSFGYGALVVLIGICAWFVWHAQTTRGTQTATDQDSKAR
                     VPLSPLPTSTLMRWIVLAFIPSSLLLGATTHITLNVAAIPLLWVVPLALYLLSFIIVF
                     AKWPAANHRIMIIALPVVLLVMAFDIMPAAPGLPYWFELFLPLGGLGVVAMVCHGELA
                     RTRPPTDRLTVFYLLMSVGGALGGLFNALIAPLVFTDIYEYEIAMVLACLILPRMESN
                     DWLRTARWSPYRLATENGQIRDWIWVLALAIVTGLSVWFIQSQEETTFLWECIFTGVP
                     LVLCYLCVGRPFRLGLSLAVVLVVANVVSAWFGNPIIYQTRTFFGVLKVEYSKEKNVR
                     KLVHGTINHGIQRMEPEARRESVSYFHPTGPYGQVFAAFSGPIAKKRIAVTGLGIAAL
                     ANYCEQGQSLTYYEIDPAVVKIAQDPTLFTYYSDTKARGVDLRVVVGDARLKMEEAEN
                     GSYDMVILDAFTSDAIPVHLLTREAMEMYLRKLAPDGLLVIHISNRYLALEPVLGNLE
                     EALGLVALKQFDFARGVPFKSGADLVVMGRRREALGPLATDARWTPLKKVKWVGVWSD
                     DFSNVVSIFRWNN"
     gene            complement(12666..13097)
                     /locus_tag="VLD60_03035"
     CDS             complement(12666..13097)
                     /locus_tag="VLD60_03035"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HSB43955.1"
                     /translation="MDLRKNFPRSMRIKLAGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     MDERLMDYAGITAEQFTATVRAIPTDEGVTAWFQQAAKPHSPAELEAWNQKLLLRGPS
                     SPESLERFKKYRDAVDPTRTDLTAWSDLQDLEEGRTVPRRS"
     gene            complement(13098..13772)
                     /locus_tag="VLD60_03040"
     CDS             complement(13098..13772)
                     /locus_tag="VLD60_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HSB43956.1"
                     /translation="MDKQKQEQPDVADDPRARELLRKAFDNTARWQKDFKGFTADLTV
                     NLNGKETRGPVMVKGPREVSVQLGNADVQKWAQEQLGMIAVHRGPRSFEESDGKYSLT
                     MEEDGHPFGIKLIIHGSNSFYRIKENRITQINRKMAHPGMPPFAFTINVEESAVTQDQ
                     KNLTTKYSVYYYSPTDGKLNNVESFTDTHVRVGSSDLPATRRIISYENGEVIVKNLTF
                     TNHHLL"
     gene            13972..14325
                     /locus_tag="VLD60_03045"
     CDS             13972..14325
                     /locus_tag="VLD60_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43957.1"
                     /translation="MERRAASHTEEEEMNQRRRLLNAAKKGDQKAVAKLFELYQVRVY
                     NSEMVNKLNRTAGTHKLQASVAKDRPGKSGRFRPSKPAPSSKSSQKKVHKPAPKTVKK
                     TKPTGSRTKKKAHKK"
     gene            complement(14334..14630)
                     /locus_tag="VLD60_03050"
     CDS             complement(14334..14630)
                     /locus_tag="VLD60_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSB43958.1"
                     /translation="MVDSPTPVAIPLATETPSTGTADGLEARVVVYNCDCHTYQQVIA
                     LFCQCIPGMTPSRAFELAWKIDHQGEAIVFEGTWKQAEEIAGKLAGGGLRVAVQ"
     gene            complement(14664..15296)
                     /locus_tag="VLD60_03055"
     CDS             complement(14664..15296)
                     /locus_tag="VLD60_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43959.1"
                     /translation="MKPASPPQVLFYPFHLCHEDTLAKLLSRFATVHFRDFMALQFTP
                     MVGMTSFADRMGDSFPELLAAGRLVQGYDVSGPLSIEVGGAIDRDLQDSTWRGIFHGS
                     LTTDRRFHRGLFEARHPLSETNSRQEHFSFSVDWVKQLCMNQKPDDSELEYALALVKT
                     SASLVYTYRLALAHGLQVGTDSAAHFALFERTSAREHIALANHLIMRKGY"
     gene            15332..16405
                     /gene="cax"
                     /locus_tag="VLD60_03060"
     CDS             15332..16405
                     /gene="cax"
                     /locus_tag="VLD60_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HSB43960.1"
                     /translation="MKKMFSSWLDVLMIFVPITIALEIAKADDLAIFLSAATAIVPLA
                     GLLGRATEHLTAHVGAGVGSLLNASLGNAAELIIALVALREGLHDVVKASLTGSILGN
                     ILLVLGAAMLAGGLKYERQQFNQTAAGMGASLLLLAAVGLIIPALFHFTAADRGPTIE
                     RELSLEISIVLFAIYVFSLMFSLRTHKHVFAGAAHDATDLGEKPWSRSQSIIVLTVVT
                     LLVAVMSEMLVGALEHAAKTLGLTQLFVGVILVALVGNAAEHSTAVMMAMKNKMDLAL
                     GIAVGSSLQIALLVAPVLVFASYLFGAPLDLIFTPFEVAAVTISVLIVGFVAMDGESH
                     WMEGVMLVGVYTMLAIAFYFLPA"
     gene            complement(16482..16682)
                     /locus_tag="VLD60_03065"
     CDS             complement(16482..16682)
                     /locus_tag="VLD60_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43961.1"
                     /translation="MPVNMDPTKKRRTRQAAAPAQTDAVGNPQAKNQFGEETEEDRIK
                     ALADAEKKNLWDATEVIDMDTF"
     gene            16790..17155
                     /locus_tag="VLD60_03070"
     CDS             16790..17155
                     /locus_tag="VLD60_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43962.1"
                     /translation="MLDAKVWWPLFPLLLLVVIVCLTWALVLVVRGEKDLRARWLQIG
                     AFACYVLAAASAVASERGLVSANLHRPFSILTQLCLFGALLHHWNGNTSSLRWLNGLA
                     WAGILADGALHFLMIRHSA"
     gene            complement(17152..18285)
                     /gene="hemW"
                     /locus_tag="VLD60_03075"
     CDS             complement(17152..18285)
                     /gene="hemW"
                     /locus_tag="VLD60_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSB43963.1"
                     /translation="MTSPIGLYLHIPFFRQRCDFCAFYLELYREGAAESFLRAMRTEI
                     RLQAEQAGIKGRPFQSIYFGGGTPTALRASQLLEILADIRRHLLLAPDCEITVEAHPG
                     TVTQSDLSILYEAGINRVSFGAESMQDDELVRIGRPALARETMAAVHWAREAGFPNIN
                     LDLMYGLPDQTLESWTSTLGQCLELAPAHLSCYALTVEEGTRLAHDIRHGRCQGPDES
                     IQIAMEQTTQTVLNEAGYQQYEISNYAKPDLACRHNLLYWTHGEYLGLGPSAQSFVHG
                     VRFGNVPNLAAYQAALAEETLPVREQTVLTEGEQLRDAVIFGLRLIQGIPTQQLRTHA
                     LNYGHAATLKALREQQLIEEEGERSRLSAQGRLHADTVADKLY"
     gene            complement(18282..18560)
                     /locus_tag="VLD60_03080"
     CDS             complement(18282..18560)
                     /locus_tag="VLD60_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43964.1"
                     /translation="MMMYQEEKTFTLRFSLEASFPEDYEGDEDRLAWVREWETFMKPQ
                     VLKQIFESLRQFPTWTSHIRNRGVSPTDEIEIVVARDYAKPVPFTLSS"
     gene            18626..18850
                     /locus_tag="VLD60_03085"
     CDS             18626..18850
                     /locus_tag="VLD60_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB43965.1"
                     /translation="MPIFEYVCRECNHRFELLIQGSIQAACPQCHATKLEKQFSAFGV
                     GATGGWAPSGGGGACGSCGDPRGPGACSMD"
     gene            18853..19173
                     /locus_tag="VLD60_03090"
     CDS             18853..19173
                     /locus_tag="VLD60_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43966.1"
                     /translation="MDSVELALQRAIATISQSDPLIKLLDQVKLGRMKPADAGLRAIV
                     ESWLMTYRKMIETASLNKQALRRIDPAPRVALLVERGLLPKDHPGAVDLQAGFDQAMT
                     HAVD"
     gene            19183..19845
                     /locus_tag="VLD60_03095"
     CDS             19183..19845
                     /locus_tag="VLD60_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43967.1"
                     /translation="MRTLVLAAVSILCVLTGIDGWAVEIGGQGVLRELSITTLHRILP
                     ADTHILVESSAIEQFLAELDGDPPDWQALYGHGHLDPGHDDRVFLLNRERDAKREGRP
                     ALSWRLAFVWAGELSPYKPELGGFPVAIGPKFIKTKWGMVRFKPEEAPSNLVALVDTL
                     QRDRLHKELAAGKTVEVDVVMTGQLIPDESLVYDFSHDEEGLGLIMPFVRVERVDFVI
                     AP"
     gene            complement(19901..20698)
                     /locus_tag="VLD60_03100"
     CDS             complement(19901..20698)
                     /locus_tag="VLD60_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSB43968.1"
                     /translation="MALIPGGEFFMGSPAGTDGLPDEQPQRKVYLGSFFIDRYEVTHE
                     AYASFVSATKHRVPENAKQASTLWNWQGPMAGIDRHPVVNVSWTDADSYCRWAGKRLP
                     TEAEWEKAARGTDGQRYPWGNEWDIHLANSASFWAGQTIEFAGGNDWDAFWIRGDGAR
                     LAKEKGLNGEVLTMPVGSFPEAVNSYGLYDMAGNVAEWVQDWYDPNYYKSAPLTNPTG
                     PERGAIKSMRGGSWLKPAISLRTSDRDWGTMDSRPSGTGFRCAQDAY"
     gene            complement(20782..21537)
                     /locus_tag="VLD60_03105"
     CDS             complement(20782..21537)
                     /locus_tag="VLD60_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSB43969.1"
                     /translation="MTRPLTKSRFQEEILRIMDQKHHWAWPAFADGTVTIEQLKRHFQ
                     QEYGVYVRDFPVLLARIHGHNPPSTVRRMLAENIYEEDTGGLSLGKSHPELFLTMMAG
                     LGLPPPTFDRISLLPAASRYRAWLDRVSSHRDWVIGAAALTVFVEGSVKDRKELAEPS
                     KPKTAEELEAIVQQHPLVLHYHLSPDAMDLIRAHQMVEAGHRHDAYDMVVNYATTLAQ
                     QRAVLRCLKKCLSLWHAYRDNVARVCGLKKPLR"
     gene            21592..21876
                     /locus_tag="VLD60_03110"
     CDS             21592..21876
                     /locus_tag="VLD60_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43970.1"
                     /translation="MKQVTVQTPEDILSLLADVALRGSGFVTECLLDYVLDEGFTEPI
                     YLNASGEDPDAYYKGQSPAWAVYQVREWKRVLTISGGPGKQRRVQITETP"
     gene            21970..22218
                     /locus_tag="VLD60_03115"
     CDS             21970..22218
                     /locus_tag="VLD60_03115"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSB43971.1"
                     /translation="MSFVHLAEPYNLGELVVLQSLLEGSGIEYVIRHAHMGSLYPGVP
                     GMNSHVMVDEHDYSRAELLLQRLRLDVREVSEEAEDSR"
     gene            complement(22215..22661)
                     /locus_tag="VLD60_03120"
     CDS             complement(22215..22661)
                     /locus_tag="VLD60_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43972.1"
                     /translation="MEQLTSAPANPAASAEEPVSFLRLRPVRLAIYAGLILFLLLMVI
                     WPLVTQMRDPNFQKHIAEHRVMVGMSKEQVLQAWGGPQTINTTFTKDGIRQEEWIFED
                     WESTSVVKHRYLYFEEGTLIGGWFQGSGERLPTQAPSNPHSPKPER"
     gene            22771..23322
                     /locus_tag="VLD60_03125"
     CDS             22771..23322
                     /locus_tag="VLD60_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43973.1"
                     /translation="MRISLTQSFRLRWSNCLAGFLLSGISLLLLDDVLCAKLLYSYRD
                     DQGTNVITDSYDRIPMQYRARVITVDQESDSLNQSGKGVADLLKGADKRIGGATIDVP
                     GLSPYQSHALTITGSLALLCFMLRQFSRSQVLRFLSLWGLVMLGLVTPVFIYLSQDGP
                     LDVLRGGASKIQTKQLEHLKDTR"
     gene            complement(23353..23988)
                     /locus_tag="VLD60_03130"
     CDS             complement(23353..23988)
                     /locus_tag="VLD60_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43974.1"
                     /translation="MPQAVRDRKSALRVFTYYAITDWDKYFRKLVSPENGEDVYTYKR
                     DGIEVRYSFSYAIDPSDQSENRPLFVRLVDIEFSPAVPFSQVPSLVPEFRPSKEPASP
                     SFRSNIWVLLFKGSSSPEARFIVKESGKESLDWTLAYQLFSLQGLPDPLTTRSLVDRM
                     EISTQSIDLVKRRQRHTHDPIMNPYSDEFSQQQTVPRPAQAKKIPLPKYAD"
     gene            24289..>25145
                     /locus_tag="VLD60_03135"
     CDS             24289..>25145
                     /locus_tag="VLD60_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSB43975.1"
                     /translation="MTALESLLRERIVILDGAMGTMIQQRKLDEAAFRGERFKDWKKD
                     LKGHNDLLNLTQPAIIEDIHRQYLEAGADVIETNTFNSQAISLADYEMETLGYEMSKA
                     GAECARRAVNAVQVAQAGRRCFVAGAIGPTTKTSSISTDVNNPGARGTTYEELVAAYS
                     DQVRGLLDGGVDALLVETIFDTLNAKAAFFAIQQAFANGGRRVPIMASVTFIQAGSNR
                     GVTGQTVEAFWNSISHVPLLSVGMNCALGPKEMRPLIGELSRIAPIYVSAHPNAGLPN
                     PLLPTGFPET"
BASE COUNT         5108 a         6735 c         7421 g         5881 t
ORIGIN      
        1 caacgcgtgt cgattgctcg cgtgctgctc aaggatcctc caatcgtcat gttcgacgaa
       61 gcgacatcga acattgacac ggaaaccgag gtcaaaatac gagaggccct ggacgagttg
      121 acaagagggc ggacgacgat cgtcatcgcc catcgtcttt caaccgttca tggtatggat
      181 cgaatcattg tgctcgagca agggcgcgtg gtggaagatg ggccgcatga tgtgctgatc
      241 gctcggggcg gggtttacgc aggtctctat gaggcgcaat tccaaaccta ggggctgatg
      301 caaatggtct tgatctatga aaatgatcca ctggataatg aacatgcccg cgggcgcttc
      361 taccacgtct gtcgcgggca ggtagaccgg acggagctgc agattccacc ggccgaccag
      421 ccgcacgaat gtgccagatg cgggatcgat ctcgaggccg aagatttttt tgtggcgatg
      481 cagaaaggat cggtatagcg acgtggctgg cagcagcgga cgcaagacgg tgtcagtgat
      541 gcggggtctg atgatgctct gcgagtcgtg ttatgatcag gtatttcttg aaaagctggt
      601 cgatcataag aactatgtgg cggatcatga agccctgttt gataccatct gcccaggctg
      661 tacggatatt aatcgtcctc tgattgacga tatgctgggt agctcggagt aatcggcggg
      721 atcagatctc ggcgcgcctc tacttgcaga ctttcccgtc gaaatacatg caggtcgact
      781 ccccggattt taccttccag gtttttagga ggggggcttg gccgcttcct ctcatctcca
      841 cgacaaaatc gaccagtcct gaaagcggca gtttctccat atgcggttgg gcggcctccg
      901 gtacgtcaat ccagaaaacc gaaccgagag tggaaatcag aatgcggtgt gcgtccttgt
      961 cgatattaat gacggggctg tagaagctct tgtcctcggc agaggaggag tttgccagga
     1021 gcaaggtgaa aaagacggag agaagaagcg tgcgcgagaa tggcataacg atcactcctt
     1081 ctaaatgagt cgataatgcg cccattatcg cactcaatca tagagaagga aactgaaatt
     1141 ctcggcgtcg acgatcgtgt ggacggacta gggagtcgtg agttgcgccg gggcaacagt
     1201 gccgcctttg atccgatagc cgcgaatgtc cggattcgat ttgtccatca acgagatcag
     1261 cagatagatc gggagggtga ggttgaggcg gctccagttc tcttcccact cattggcaat
     1321 cttgatgtcg gttacagagg ggcgtgctgg atcatgcgga tgagagtggt agaccacttg
     1381 tagcgtcagc ccaagtttcc gcatatcttt cacggcctga aacatctcct gggcgtccat
     1441 gatgaaggca atctctgccc gctcttcggg ggacaggcgg gacagatgct ggacctttgc
     1501 gtcgtcgaat gaagcgactt gggcagctcc gtcgagactg acgatgttct tgatgcgata
     1561 gatgtggctg acggtgtggt ccttacctgc caagaacccg caacattcat agggatcgag
     1621 ttcccgtgcg tgggccacaa ggtcgtcaag aatctgttgg ggaatgatga gatccgacac
     1681 ggctagattg tgcagctgac ggtgtagtcg ctgttcaaat cctttatggt cggatttggt
     1741 ccacagagac ggcaggcggg gtccttcgga cggccgacct tccgaaactt catgtcgagc
     1801 gcgtcgtaaa ttagcagctt atccgcgatc gtttcaccga tccccagaat ttccttgatg
     1861 gcttcagagg cctggagaat ccccatcgtt cccgctaaga cgccgaggac accagcctcc
     1921 tggcagttgg gcacgagtcc cgcaggagga ggttccgggt aaaggcatcg atagcacgga
     1981 tatcccgcat gcggcttgat cgtggtgagt tgtccctcga agcgaaacat gctggctgag
     2041 acgagcgtct ttttggcaaa gaaacaggca tcgttgacca gaaaacgggt cgtgaaatta
     2101 tcggacccat cgagcacgac gtcatagtct ctaaccaggg agaggatgtt ctcggcgtca
     2161 accaactggt gataggcgtt gacggtaatt tccggattga cggcatggag ggtcttccgg
     2221 cctgattcga ccttgggttg cccaaccgtg gccgtcgaat gaagtatctg ccgttgcaag
     2281 tttgagagat cgaccacgtc gccatcgacc aggccgatcg tcccgacacc cgctgcagca
     2341 agatacaggg cggcagggga gccgagcccc ccggccccga tgagcaacac ctttgccttg
     2401 ctcagcttga gttgcccttt gccgccgacg tcttgaagga taatgtggcg gctgtagcgc
     2461 tgaatttgtt gctcggtgag ctccatttat tactccacca cgttcaattc aatcgggtcg
     2521 acgacgcaac ccttctttcg aagaccttca atggcccgtt cgatttctgc agtttcaccg
     2581 gaaatttcaa catccaccca tccggtggtt tcgcgcacgt cagcgcgccg cacgttcgtg
     2641 acgatgttgt actcacggcc gatctgatag atgatcggct ccttgacctt atcttccgga
     2701 aaccgcacgt ggaatcgtag gtgcgacatg atcagcctcc cgcaatcgcg ggcacgatag
     2761 agacttcgtc gccgtccttc agcgaggtct ccttaccctg cagaaaacga atgtcttcct
     2821 cgttcacgta aatgttcacg aaacgccgca attcaccgga ctcatcgcag agccggtcct
     2881 tcaagccagg atgcgactgg ttcaacgttt cgatcatgtt ggcgatgctg ctcgcctgtg
     2941 cctcgacctc gccctgtccc ttcgtgaggg gacggagagg ggttggaatg cgaatcttaa
     3001 tcatgcgtca ccaccaccgt tctttcccat cttgaatgtt ttttcaaatg cgaccaggct
     3061 cggctgaatg cgaaccggcc tgcccactgc atcgatcacg gcttcctgcg tcttcagccc
     3121 gttgccggtg atataggcca cggtgacgtc gcctttcttg atcgtcccgt tctttacaag
     3181 cttttgaagc acaccgatcg tgacaccgcc ggctgtttcg gcaaagatcc cttccgtctg
     3241 cgccaggagc ttaatgcctt ccaccacctc gtcgtcggtc accatgtcca tcgagccatt
     3301 actctctgca gtggccttca aggcgtagta gccgtcagcc ggattgccga tggccaatga
     3361 ctttgcgata gttttgggtt tcaccggttt gaagaaatcg cgccccgctt tgaacgccgt
     3421 cgaaatgggg gaacagcctt cggcctgcgc cccgttgatc ttggtccgga cctcatcgat
     3481 cagccccacg gccttcattt cgtgcaggcc cttccagatt ttcgtcagca aggaacccga
     3541 tgccatgggg atgacggctt gatccggagt gcgccagccg agttgttcaa ccgtttcaaa
     3601 ggccagcgtc ttcgatcctt cggcgtaata gggacggatg ttgatgttca cgaatgccca
     3661 gccatgttcg ccggcgatct cgctgcagag ccggttcacg tcatcgtagt ggccttcgat
     3721 ttccaccacg ttcggcttat agattaagtt acccagaact ttggcagcct ccagatcgcc
     3781 agggatgaag acatagcagc gcatgcccgc cgcggccgca tgggctgcga cggagttggc
     3841 cagattgccg gtcgatgcgc aagccacggt ttcaaagccg agctcgcggg cgcgggtaat
     3901 cgcgacagcc acgacgcgat ccttgaacga aagagtcggg tgattgacgg tatcgttctt
     3961 aatgtacagc tcgtcgaggc ccaagtacgc gcccagattc tttgcacgca cgagcggtgt
     4021 catccctgca tgcgggccaa ggtaattggg gccttcgacg gggagcaaat cggcatagcg
     4081 ccagatgctc tgtgggcctt ccccgatttt tttcctggag atcgagcgct tgatctcgtc
     4141 gtagttgtat ttcacctcga gcggaccgaa gcacatctcg cagacgtgaa tggccttcgt
     4201 cggatattct ttcccgcact cgcggcacac tagcgctttc attttggcca taggcacacc
     4261 accttcgtga cgtcaatgat tacggtcgta cggcacagcc tgcaaacgga tcgccatcgt
     4321 cgaacagctc ggtgatcgtc ggatgctcac cgcagagcgc acagttgggg ttgcgcttga
     4381 ctttgatttc tctgaactgc gttttacgtg catcgaagtc cagcaggcga tcggtcaatg
     4441 ggcggccgat acccagcacg agtttaagcg cttccgtcgc ttgaatcgtc ccgataatac
     4501 ccgcaaggac cccgatgact ccagcctcct ggcaggaggc caccagccct ggcggcggtg
     4561 gcgtcttgaa aacgcaacgg tagcaggcgg attttctcgg gataatggtc gtgacgcgtc
     4621 cgtcgaaccg gaggatgcca ccatgaatca acggtttgtc ggcaaagaag caggcatcgt
     4681 tgatcaagaa cttggcggtg aagttatcga ctccgtcgat gaccacatcg tagtcgttga
     4741 tgatcttgat cgcattgcct gcggtcagcc gctcctcata catggagacg gccacgtcgg
     4801 gattgagggc ctggattttt tcttttcccg acaacacctt gggccgcccg atgtcggggg
     4861 tgtggtgcaa aatctggcgt tgcaggttcg tgagatccac gacgtcgctg tcgatcaggc
     4921 cgatgcgccc gaccccggcg gctgccagat acagcgcggc tggagaaccc aggccgccgg
     4981 cgccgacaag aagaattttc gccttggcga ttttcttctg tccctttccc ccgacctccg
     5041 gcaacagtat gtgccggcta tagcgggaga tttgttcttc ggtgaattcc atggttgtca
     5101 gatattaaag tatttctcga tactgatata ccgctcaccg gtgtcacaca ggatcgttac
     5161 gacgttcttg ccggggccga gttcttcggc gatcttctgt gcggcaaaga cgttagcccc
     5221 cgccgagatg ccgaccagaa gtccttcttg ctttgagagc tgcttcgccg tctgataggc
     5281 ttcgtcgtcg gtgacggtca gaatcttatc aataatcttc ctgttcagga ccttgggaat
     5341 aaaacctgcg ccgatacctt gaatcttgtg cgggcccggg tcgcctcctg acagcaccgg
     5401 tgaaccggcc ggttcgaccg ctacgacctt cacattggga ttccgttcct taaagacctc
     5461 gccacagccg gtgatggtgc cgccggtccc gactgctgcg acgaaggcat cgatctttcc
     5521 atccagcgcg tcgagtattt ccggcgccgt agtcatccgg tgcatggccg gatttgccgg
     5581 gttcgaaaac tggtccggca tgaagtacga cggattttgc gccaacagac tttccgcctc
     5641 tcgtatcgat cccttcatgc cttcccacgc aggcgtcaga acgagctggg ctccatagga
     5701 tgagagaagg cttgcccgtt ccatgctcat gctttccggc ataaccaaga tcagcttgta
     5761 ccccctgacg gccgcaatca atgcgagtcc gatgccggtg ttgccgctgg tcggctcgac
     5821 aatggtgccg cccggcttca gcttgccttg ccgctcggcc tcgttgatca tattgaggca
     5881 gatacggtct ttcacgctcc cgccgggatt aaaaaattcg accttcccgt agattgtcgc
     5941 gcagccgggt ttggaaagcc ggttgaggcg aacgagggga gtccgaccga tcagttcggt
     6001 aatctcctta tgcaagatca tagtcatcga ccgccaccca tgtagaacaa aaattcaagg
     6061 cggtcgccat cattgaggtg gatcgttcca aggtgctcgc gatccaccat ggcatcgttg
     6121 acctcgactg ccaccatctg cggttcgatt ttcttggtct tgaggagatc cagaacggtg
     6181 ccccccggaa actcctcagc cttgccgtta attttgacct gcatgaaaac tcctaacttg
     6241 gcccagggat taaagaattt ggacagagta acaaactgcc ttccggcttg tcaaggcaac
     6301 ggctcgcgcc ggtccgttgc ttgacattgg tcctccagcg cctccactat gcctcctttt
     6361 ggagggcctt gttcatcatg tggaaacgca acttcatgtt tcgggcatcc gatgcgacgc
     6421 cgctcaaaga atccgaaaac gaactgtttc acgagactga cccggcaatg gatagcgtcg
     6481 ggttgcagtt agagaagttt ttatccgttt ggattcaagg tgagggagac gacgagaaac
     6541 cgtctgcctt taccaacatg tatgtccgca cggcgacctt ggatttccag aagcgggtgg
     6601 gctttcttca gccgttacaa ggacggacgc atcagattaa gcaattgctc acgccgggtc
     6661 agaaggagtt tttgcgcaat tggctttcca cgaatgcgcc gcaggcctgg gattcgaccg
     6721 acgaacattt caagatgttg ttcgaactcg aatgagccat cgtgatgcct ctacctccag
     6781 atttcctcgc cggagccggt ttctattctg cgggctgctc tgttcgctct tgctggcgca
     6841 ggaaccaaag gcctggggcg ccggggtcga ggtctactac tcacctgaag atgcgccgct
     6901 cgatcgagtc gtcagcctct atgagcatgc caaacgttat atctatgtgt cggtctacgg
     6961 attgacgtct ccacgcgccg tcgaggctct ggtcgcggcg aagaagcgcg gcctggacgt
     7021 tcgcttcatt accgaccagg agcgtgcgca agaggtgaag caacgcacgg cgctgcacac
     7081 gcttcgtctg gccggcattc ccatccaggt caatcaacac gatgggttga tgcatctgaa
     7141 gcaagtggtc atcgatgacg aagtgaacac gtccggttcc atgaaccaca cgacgagcgg
     7201 caatcgatac aacgatgagc ggatggacgt catcacggac catgcgatca ccgtgaaggc
     7261 ccgcgaaaag tttctcgcca tgtggaatga ccatgagcgg tatcgcgcct ggaccgatga
     7321 ctgaccgcca tgacagaacg acttgaacag acggatgtgg tcgttgtcgg cgccggtggc
     7381 ggcggagcgg tgctcgccct ggctttggct cagaagggta tcaacacgat cgtgctggat
     7441 catgcgcagg ggcctccgca gggattgcgg ggagaaattc tccaaccgaa cggacaatcc
     7501 gtgttggatc gcttgggtgt cttgcagtcc ttgcccactg aggcgactcg ctcggtgcgg
     7561 aactttcact tctgccgagc cggtgggaaa cgcctgtgct cgatcgatta tggcgacctg
     7621 cccgcaccgt acaatcgagc gatcgtgacg ttgccgaatg tggcccatca cgccattgtc
     7681 gatgccgtcc agcagcagcc cagcgccagc cttcgatacg gaacgtcttt cgccgggttg
     7741 cttcgagaag ggggccgagt tgtcggagtg accacacaag gttcggaggg tacgcgaacg
     7801 attcgagcca aactggtggt cggcgccgac ggcgcctttt caaaagtgcg tgaagcctta
     7861 aagatccctg ccgatgtgta tttctatccg gaaggttacc tcattgccat cgtggaatca
     7921 gaggagccgg tttcggagtc tttctattat gtcggtcatc ggcagattct cgggttattt
     7981 cccgcgaccg gcaacaaggt ctatctgttc tacatgattc ccagcggatc ttacgaggca
     8041 gtgacgcaac gcggcatcgc ctcgctgcag caagcctggt cgaacatcgc gccgcagttt
     8101 gcgaagctgt ttcagaatct caccgattgg agtcagaccg cttacatgcc gaccggtcgc
     8161 gtaaggacgc ctacctgggt ggctgacgga gcggtgctga tcggcgacgc ggcccacgcc
     8221 atgaaccctc acgcatcgca gggacgcatg caggcgatgg tcgacgccat ggcgctggcg
     8281 gatcttctcc cggcttgtct ggcggacaat gattgttccg ctgctcgcct gaaaccgtac
     8341 gagactgttc gacgtccgca tgtgacaatg ttacaacgct tggctgatca gcaagtgttt
     8401 tactggaaca ccgggaatcc ggtggttgcg tacctgcgcg atcgcgtgtt tcaaaccttg
     8461 gatcggaacc cgcgtcttcg ctatcaagtg ctttcgacga cggccgggtt gagaccgacc
     8521 gcgccgttca atctgataga tcgtgtcatt gccgcgggac tgctgccgga tctgcgagcg
     8581 agcgggagag cctcccatcc gatgtcttca tgaggaagca tgagcacgac tgaacgtgtg
     8641 atcgagggat taccggagga aacgcagaac ctcttgtacg cctatgtgaa ggacgtgaaa
     8701 ggtctcatcg gtgagcaatt ggaaggcgta ttgctctacg gcagtgcggt gcgtggcgaa
     8761 ttcttgcagg gtcggtcgaa cctgaatttg ctcctctttg tgtcttcgta tgatcaagcc
     8821 ttgttgaaag gatacagcgc catccatcga cggtggagca aggagcaaat cgttgcgccg
     8881 ctgttcctca cggaagagga gctgcgctcg tcggcctccg tctttccgct ggaatatctc
     8941 gagatccagg aacagcatcg tgtgttgggt gggcgtgatc cattcgttgg atttcgtgtc
     9001 gacacacaga ggttggcggc agaggtgcgg cagggtgtgg tcggagcgtt acttcgtctt
     9061 cggcagcgct atgtggaggg gaacgcgacc gatgaagcgg cgctcatcct cttgccgctc
     9121 tcggtgaccg ctctgttgcc gctactccga ggggtgcaac gcttacaggg gcgcgccgtg
     9181 acgtatcaat ccgatgcggt ggtcaaggac gtggcggaac tgctcaagct ggatctgcag
     9241 ggtttccacg atgcgctctt gctcaagcga ggacaaatta cccctgggcc aaaagaaatc
     9301 ccgcgcctct tcgggcgcta tctgcaagcc ctcacggcgc tcgcgaacag ggtgacgggt
     9361 ctcgtgccgg acggtgaccg atgaagatct ggcttgccct cctcatactg gtgttgttcg
     9421 gtgggcatgc ggaagctgtt ccgtacgacc ggcccaaaat tcaactgccg gatccgcgcg
     9481 ggtatgtgtc tgactatgcg caagtgctcg acggtgattg gaaggaaagg atccggtccg
     9541 tctgccaaga tctcgaacgt aaaacgggcg tcgagatggt tgtcgtgacc gtaccgtcta
     9601 tcaagccttt cgggtcggcc aatgaattcg ctaccgcgat ctatgaaaaa tggggcattg
     9661 ggtcgacgca acaggaacac ggcgtattgg tattagtggc cgtgcaggaa cggcaggcag
     9721 ccgtgacgct gggccgtcag atgctgccga taattacgcc ggccgtgatg aatcaggtcg
     9781 gtggcgaata tcttcatccc tcgatcaatc tcggccattt cggtgaagga ctctatcgca
     9841 cggtcgtggt gctggcctcc cgctcgcagg aggttcgtgt cggatcgctc tcgaaaactc
     9901 actttaaagg tgtgggtttc tggatcacgc tcttcactag catcggcgcc ctttcgtttc
     9961 tctggtggat cagtcggccg gacatgcgcc atccttatag ccgcatcaga aaaggggagt
    10021 attggggaac ggggcaggga ggattcggcg gaaactgggg cgggtttggt ggagggacga
    10081 gcggtgaagg gtttcggtag agaagagtag ccgcccacct agaagtagga cacgtatgat
    10141 aagagggcgt ttcttcgacc tgactgggtt ctcttctgcc agtcacgatc atgatagaca
    10201 accatcgtcg aaggtcaagt aggttgagcc agagattttg tggtcacggg cacgggtcaa
    10261 cgtcctgtct gcaagcctgt atctagttca atcgattttc tgctgatcca gaatctgagc
    10321 gatgttgtga gcatcaagcg gaggaatgca tggcgatagt gtggtcggtg acgatctttc
    10381 tgagcgcgtt cttgttgttc ctcgtgcagc ccatgatggc gaagatgatt ctgccgatgc
    10441 tgggcgggac tccggcggta tggaatacct gcatgctgtt ctatcaggcg acattgctgg
    10501 ccgggtacgg ttacgtccat ctcctcaacg ctcgcgtggc tcctcgccgg cagctccctg
    10561 ttcatcttgc cttgctggcg ttgccgctgc tgctgctgcc gattggcctt ccggcgggct
    10621 ggactcctcc cgatgagtcg aatcctgtgt tctgggtact tctgctcctg acggtcgcga
    10681 tcgggctccc gttcttcgtg ctttccacca cggcgcctct gttgcagaag tggttttcct
    10741 ggacggatca tccatcggca ggagatccgt actttctata tgccgcgagc aatgccggaa
    10801 gcatgctcgc gctgcttgga tatccgttgt tgatcgaacc gtggcttccg ctgaaaggct
    10861 cagaacgact ggcgcagacg acgttctgga gtttcggata cggtgcattg gtcgttctca
    10921 tcggcatctg tgcatggttt gtctggcatg ctcaaacgac caggggaacg caaacggcga
    10981 ccgatcagga ctcgaaggcg agggttcctc tctcgccttt gccgacgagc acgctcatgc
    11041 gatggatcgt cctggctttt atcccttcca gcctgcttct cggagcgacg acccatatca
    11101 cgctcaacgt ggcggcgatt cctttgctgt gggtcgttcc cttggcgctg tatctcctca
    11161 gtttcatcat cgtgtttgcg aaatggccgg cggccaatca ccggatcatg attattgccc
    11221 tcccggtggt cttgttagtg atggcctttg acatcatgcc cgccgcacca ggcttgccct
    11281 attggttcga actattcctc cctctcgggg gattgggcgt ggtggccatg gtctgtcatg
    11341 gtgaactggc gagaaccagg ccgccgacgg accgactcac ggtgttttat ctcctcatgt
    11401 ccgttggggg agccctcggc ggtctcttca atgcgttgat tgccccgcta gtttttacgg
    11461 atatctatga gtatgagatc gccatggtgc tcgcctgctt gatcctgccg cgcatggaga
    11521 gcaacgattg gcttcgaact gcgcgatggt cgccgtaccg gttggcgaca gaaaatggcc
    11581 agattcgaga ttggatctgg gtgcttgctc tcgcaatcgt gacaggccta tctgtttggt
    11641 tcattcagtc gcaggaggaa acgacatttc tctgggaatg tatctttaca ggcgttccgc
    11701 tggtcctgtg ctacctctgt gtgggacgac ctttccgcct gggactgagt ctggctgttg
    11761 tgctggtggt ggcgaacgtt gtcagcgcct ggtttggaaa cccgataatt taccaaacgc
    11821 gcacgttctt cggcgtgttg aaagtggaat attccaagga aaagaacgtg cgcaagttgg
    11881 tacatggcac gatcaatcac ggaatccagc ggatggaacc ggaagcccgg cgtgaatccg
    11941 tgagctattt ccatccgacc ggtccgtacg gacaagtctt cgccgcattc agcgggccga
    12001 ttgccaagaa aagaattgct gtgaccggct tgggcattgc tgcgttggcg aattactgcg
    12061 agcagggtca atctttgacg tattacgaaa tcgatcccgc cgtggtgaag atcgctcaag
    12121 accccacgct ctttacgtac tacagcgaca cgaaggcgcg aggagtcgat ctgcgtgtcg
    12181 tggtcggtga tgcccggctg aaaatggagg aagcggaaaa cggctcgtat gacatggtca
    12241 ttctggatgc ctttacctcc gatgcgattc ctgtgcatct cctgacccgc gaagccatgg
    12301 agatgtatct tcggaagctg gctcccgacg ggctcttggt cattcacatc tcaaatcgat
    12361 atttggcgct tgagccggtg ctggggaacc tggaggaagc gctcggtttg gtggcgctca
    12421 agcaatttga ctttgcgaga ggtgtcccgt ttaagtccgg agccgatctg gtggtgatgg
    12481 gacggcgccg tgaggcgtta ggtccgctcg ccacggatgc ccggtggacc cccttgaaaa
    12541 aggtcaaatg ggtcggggtg tggtcggatg atttctcgaa tgttgtgagt atcttccgat
    12601 ggaacaacta gggaggccgg ccgaccccgt tctcgatggg gcctggtgga gaatgccagg
    12661 ttgtcttaag accgtcttgg taccgttcga ccttcttcga gatcctgcaa gtccgaccag
    12721 gccgtgagat cggttcgtgt ggggtcaacg gcgtcgcggt atttcttgaa tcgttccagg
    12781 ctctccggag aactcggccc acgcagcagg agcttctggt tccatgcttc caactcggca
    12841 gggctatgcg gctttgcagc ctgctgaaac catgctgtca cgccttcatc ggtggggatg
    12901 gctctcacgg ttgcggtgaa ttgctcggcg gtaataccgg cgtagtccat gagccgttca
    12961 tccataggac aggggtagat gtactcgcct tcggtgccgg cgaggacagc tcggcacttg
    13021 tcgatcatgc gcgccaagtg cacataaccg gcaagcttga tgcgcatgct tcgaggaaag
    13081 ttcttccgca gatccattta gagcaggtgg tgatttgtga atgtaagatt tttcacaatg
    13141 acttcgccat tctcatagga aatgatgcgc cgggtagccg gtagatccga tgatcccacg
    13201 cgcacgtgag tgtcggtgaa gctctccacg ttgttcagtt tcccatcggt gggcgaatag
    13261 taatagacgc tgtacttggt cgtgagattc ttctgatcct gggtcacggc actttcttcg
    13321 acattgatcg taaaggcgaa gggaggcatg cctggatgcg ccattttccg gttgatctgg
    13381 gtgatccggt tttctttgat tcggtagaac gagttcgatc cgtggatgat gagcttgatg
    13441 ccgaacgggt gcccatcttc ctccatcgtg agggagtatt taccgtccga ttcctcgaaa
    13501 ctgcgggggc ctcgatggac ggcgatcatg ccgagttgtt cctgcgccca tttctggaca
    13561 tcggcgtttc caagctggac ggagacttcg cgcgggcctt tcaccatgac ggggccccgg
    13621 gtttctttgc cgttcaagtt gacagtgagg tcggcggtga accctttgaa gtccttttgc
    13681 cagcgagccg tgttgtcaaa cgccttccga agcaactcgc gggcacgagg gtcatcggcg
    13741 acatctggct gttcctgttt ctgcttgtcc atgatcgggt ctccttatag cgctgaagtg
    13801 tgaatgtgga attatacccg tcccatcgac cgccactcaa tgccggtcga gcggcttgcg
    13861 ccggcgcaat cgatattttc aatgtgcggc cgtttctgat atagttctgc gattttttcc
    13921 ggtcgagtgt tggtaaggga ctcttgcaac gataagggag agaggatcgg catggaacga
    13981 cgggcggctt cccatactga agaagaagaa atgaaccagc ggcggcgtct cctgaacgcc
    14041 gcgaaaaaag gcgatcagaa ggcggtggcc aaactcttcg agctgtatca agtcagagta
    14101 tacaacagcg agatggtcaa taaactcaat cgaaccgccg gtacgcataa actgcaagcg
    14161 tccgtggcca aagacaggcc gggaaaatcc gggaggttca ggccttccaa accggcgccg
    14221 agttcgaagt cgagtcagaa gaaggtgcac aagccggcac ccaagactgt caagaagacg
    14281 aaacctaccg gctcgcgaac caagaaaaaa gcgcacaaaa aataatcgga tcgctactgt
    14341 accgccacgc gaagcccgcc ccccgcaagc tttccggcta tttcctccgc ctgtttccag
    14401 gttccctcga agacgattgc ctctccctga tgatcgattt tccaggccag ctcgaaggcc
    14461 ctggagggag tcatcccggg aatgcattgg caaaacagcg caatgacctg ttggtacgtg
    14521 tggcaatcgc aattatagac cacgacgcgc gcttcaaggc catcagcggt tccggtagac
    14581 ggggtctcag tcgctagagg gatcgcaacg ggggtgggag agtcgaccat agcgcgggat
    14641 tttagcagac tttcgcgggc tgattaatat ccttttctca tgatgagatg gttcgccagt
    14701 gcaatgtgct ctcgggcact ggtccgttcg aacaatgcaa agtgggccgc agaatccgtg
    14761 ccgacctgaa gaccatgggc cagtgcgagc cgataggtat agaccaagga ggccgatgtt
    14821 ttgacgagtg cgagggcata ttcgagttcc gaatcatcag gcttctggtt catacagagt
    14881 tgtttgaccc aatctacgct gaaggagaaa tgttcctgcc ggctgttcgt ttcagagagt
    14941 ggatggcggg cttcgaagag gccgcgatga aatcgacgat cggtcgtaag ggatccgtgg
    15001 aagatcccgc gccaggttga atcctgcaag tcacgatcaa tcgcgccacc gacctcgatc
    15061 gagagcgggc cgcttacgtc gtagccttgg acgaggcgtc cggcagcaag aagttcgggg
    15121 aaactgtcgc ccatacggtc ggcgaaggat gtcatgccga ccatgggggt aaattgtagc
    15181 gccatgaagt cgcggaaatg cacggtggcg aagcgcgaca agagcttggc caatgtgtct
    15241 tcgtggcaaa gatgaaacgg atagaacagt acctgtggcg gtgatgccgg tttcatcgac
    15301 aaagattccg ggtcatgcta acgttcgccc catgaaaaaa atgttttctt cctggctcga
    15361 cgtactgatg attttcgttc cgatcacgat cgctctggag atcgcaaagg ccgatgatct
    15421 tgcgatcttt ctctcggctg cgacggcgat cgtgccgctt gccggtctac tggggcgggc
    15481 cacagaacac ctgaccgcgc atgtcggagc aggggtggga agtctcctca acgcctcgct
    15541 cggcaatgcg gctgagttga tcatcgcatt ggtcgccctg cgcgagggct tgcacgatgt
    15601 ggtgaaagcc tctcttaccg gttcaatatt gggcaatatc ttgctcgttc ttggggccgc
    15661 aatgctcgcg ggcggtctca agtatgaacg gcaacaattc aatcaaactg ccgcaggcat
    15721 gggggcgagc cttctgctct tggcggccgt cggtctcatt atacccgcgc tctttcactt
    15781 cacggccgca gatcgtggac ccaccattga gcgggagtta agccttgaaa tttccatagt
    15841 gttattcgcc atttatgtct ttagtctcat gtttagcttg cgaactcata agcacgtatt
    15901 tgccggagcg gcccacgatg cgacggatct gggagagaaa ccctggagtc gttcgcagtc
    15961 gatcatcgtg ctcactgtgg tgacgttgct ggttgcggtg atgagcgaga tgctcgtggg
    16021 ggcgttggag catgccgcca agacgctggg gctgacgcag ctcttcgtcg gggtgatcct
    16081 tgtcgcactc gtcggcaacg ccgcggaaca ttcgactgcg gtcatgatgg cgatgaagaa
    16141 caaaatggat ttggccctcg ggatcgccgt ggggtcgagt ttgcagatcg cgctgcttgt
    16201 ggcgccggtc ttggtgttcg ccagctatct gttcggtgct ccgttggatc ttatttttac
    16261 gcccttcgaa gttgcagctg tgacgatctc ggtgctgatc gtcggctttg tcgcaatgga
    16321 tggcgagtca cactggatgg aaggggtcat gctggtgggt gtgtatacga tgctggcgat
    16381 tgcgttttac ttcttgccgg cataaacgct cacagccttc agggatcagc cgctactgtt
    16441 tttatggtga tggctgattg cctagcactg ccaggcgtgt tctagaaggt gtccatgtcg
    16501 atgacttcag tagcgtccca gagatttttc ttctccgcat cggccagagc cttgatccgg
    16561 tcttcttccg tttcttctcc aaactggttc ttcgcttgcg gattgcctac cgcatcggtc
    16621 tgagcgggag ctgcggcctg tcgcgtccga cgctttttgg ttgggtccat attgacgggc
    16681 atggggcctc caaaatgatg ttttagtctc caccctggtg gtagaaaaag tcaatggagg
    16741 ttcatcctct cgttgacctc ctcagaaagg cttgttagag ttcgccccca tgctggatgc
    16801 caaagtctgg tggcccctat tccccctgct gctgctcgtc gtcattgtct gtttgacgtg
    16861 ggcgctggta ttggtcgtgc gaggtgagaa agaccttcgt gcgcggtggt tgcaaatcgg
    16921 ggcttttgct tgctatgtgc tggcagccgc ctcagctgtc gccagcgagc gaggtttggt
    16981 ttcggccaac ctgcatcgcc ccttttcgat cctcacccaa ctctgcttgt tcggcgcgtt
    17041 gctccatcac tggaatggga acacttcctc gctgcgctgg ttgaatgggc tggcttgggc
    17101 tggcattctg gcggacggcg cacttcattt cctgatgatt aggcactcag cctagtacag
    17161 tttatcagcg accgtatcgg cgtgcaggcg gccttgggct gataggcggc tgcgctcacc
    17221 ttcttcttca atcagttgtt gttccctaag ggccttcaac gtcgccgcgt gcccgtagtt
    17281 taatgcatgg gtgcgcagtt gttgtgtcgg gataccttgg atgagccgaa ggccgaagat
    17341 caccgcatcg cgaagttgtt ccccctccgt aagtacagtc tgttcccgga ccgggagagt
    17401 ttcttcagcc aaagccgctt gataggcggc cagattcggg acattcccaa accgaacgcc
    17461 gtgcacgaac gattgtgcgc tcggtccgag tccaagatat tcgccgtgag tccaatacaa
    17521 cagattatgc cggcatgcga gatcaggctt ggcgtagttg gagatttcat attgctggta
    17581 tcctgcttcg ttgagtacgg tctgggttgt ttgttccatc gcaatctgga tcgattcgtc
    17641 cggaccctgg catcgaccat gtcgaatgtc atgggcgagc ctcgtgcctt cctctacagt
    17701 caatgcgtaa caggagagat gtgccggggc cagttccaga cactgaccga gtgtgctggt
    17761 ccagctctca agcgtctgat ccggaaggcc atacattaaa tccagattga tgttggggaa
    17821 tcctgcttct cgagcccagt ggacagccgc catggtttca cgagcgaggg caggtcggcc
    17881 gatgcgaacc agctcgtcgt cctgcatcga ctctgcgcca aagctgacgc ggttgatgcc
    17941 ggcctcatac aatatcgaga ggtccgattg agtcactgtg ccgggatggg cctcgacggt
    18001 gatctcgcaa tcgggagcca ggagcaggtg tcggcgtatg tcggcgagaa tctcaaggag
    18061 ttggcttgct ctgagcgcgg tgggtgttcc tccgccgaag taaatcgatt gaaagggtcg
    18121 ccccttgatg cccgcttgtt cggcttgcag ccggatttcc gttctcatcg ctcggagaaa
    18181 tgattccgct gcaccttccc gatagagctc cagataaaaa gcgcaaaaat cgcagcgttg
    18241 gcggaagaac gggatgtgaa gatagaggcc gatggggcta gtcatgacga gagcgtaaat
    18301 ggaacaggtt ttgcgtagtc tcgtgccacc acgatttcga tttcatctgt tggcgagacc
    18361 ccgcgattgc ggatgtgtga tgtccatgtt gggaattgcc gaagcgattc aaagatctgt
    18421 ttgagtacct gtggtttcat gaaggtttcc cactcgcgca cccaggcgag acggtcttcg
    18481 tctccctcgt aatcttccgg gaaagaggct tccagactga atcggagcgt gaatgttttt
    18541 tcttcctgat acatcatcat cctcacgcgt tgcgaagtgc gctcatgcgc ttctataata
    18601 gagcgttcaa caaggagtgg aacttatgcc tatcttcgaa tatgtgtgcc gggaatgcaa
    18661 tcaccgcttc gaactattga tccaaggctc gattcaagcc gcttgtcccc agtgtcacgc
    18721 cactaagctg gaaaagcagt tctccgcgtt cggtgtcggt gcgacgggag gctgggcacc
    18781 gtcaggcggg ggaggtgcct gcggtagttg cggcgatccc cgcgggcccg gggcctgctc
    18841 gatggactaa tcatggattc cgtcgagctc gcgctgcaac gagcgattgc gaccatttct
    18901 cagtccgatc cgctcatcaa actccttgat caagtgaagc tggggcggat gaaaccagct
    18961 gatgccgggt tacgggcgat cgtcgaatcc tggttgatga cctaccggaa gatgattgaa
    19021 acggccagct tgaataaaca ggcgcttcga cgaattgatc ccgcgccacg ggtggcgctg
    19081 ctagtggaga gaggcctatt acccaaagat catccgggcg ccgtggactt gcaggccggt
    19141 ttcgatcagg caatgactca tgctgtcgac tagcgacttc gtatgcgcac ccttgtcctt
    19201 gccgcagtat cgattctctg cgtattgacg ggaatcgacg gatgggccgt ggaaatcggc
    19261 ggacaaggtg tgttgcgaga actctccatc actaccctcc accggattct tccggcagac
    19321 acccacattt tggtggaatc ttcggccatc gagcagtttc ttgccgaatt ggacggcgat
    19381 ccgccggatt ggcaagcgct ttatggccat gggcatcttg acccgggtca tgacgacaga
    19441 gtctttttgc tgaacaggga acgcgatgcg aagcgcgagg ggcgtccggc attgtcgtgg
    19501 cgcctggcgt ttgtgtgggc gggggaattg tcgccgtaca agcctgaact cggcggtttc
    19561 cctgtcgcga ttgggccgaa atttatcaag acaaaatggg gtatggttcg tttcaagccc
    19621 gaggaagctc cgagcaattt ggtagcactg gtggatacgc tgcaacgtga ccggttgcac
    19681 aaagagttag ctgccggcaa aacggttgaa gtcgacgtcg tgatgaccgg acaactgatt
    19741 cccgacgaat ccctcgtgta tgacttttcc catgatgagg aaggactcgg cctcatcatg
    19801 ccattcgtgc gcgtcgagcg agtggacttc gttatagccc cataaacaga aaatcaaaaa
    19861 agtccgaagg gccgtgtctc cgggcaaggc ctgatgttca ttaataggca tcttgagcgc
    19921 aacgaaaccc tgtcccgctg ggcctgctgt ccatcgtgcc ccaatcacga tcgctggtcc
    19981 gaaggctgat ggcaggcttg agccatgatc ccccccgcat tgatttaatc gctccacgct
    20041 ctggaccggt gggattcgtc agtggagccg acttatagta attcggatcg taccagtcct
    20101 gtacccattc agcgacgttg ccagccatgt catagagacc ataggaattc accgcttcag
    20161 gaaagcttcc gaccggcatc gttaagactt caccattgag ccccttctcc tttgcgagac
    20221 gggccccgtc gcctcggatc caaaaagcat cccaatcgtt gccgccggcg aattcaatcg
    20281 tttgtccggc ccagaagctg gcgctgttcg caagatgaat gtcccattca tttccccatg
    20341 gatagcgctg tccgtccgtc ccgcgcgcgg ctttttccca ctcggcttcc gttggaaggc
    20401 gcttgccggc ccaacggcaa taagaatccg catcggtcca actcacattg acgactggat
    20461 gtcgatcgat tcccgccatc ggaccttgcc agttccagag ggttgaagcc tgcttggcgt
    20521 tttccggtac gcgatgcttg gttgctgaga cgaaggacgc ataggcctca tgtgtcactt
    20581 cgtaacggtc gatgaaaaaa ctgccgaggt acacctttcg ttgaggctgt tcatccggga
    20641 gcccgtcggt gccggctggg gaacccatga aaaattcgcc acccggaatc agcgccatac
    20701 cctcaggcgg agaggagcct atggccggcc gcaaaaagat gactgagagc agcaggagga
    20761 taagcgttct gagacgcaag gctatcgcaa aggttttttg agaccgcata cgcgagccac
    20821 gttgtcgcgg taggcatgcc acagggagag gcacttcttg agacagcgca acacggcccg
    20881 ctgttgcgca agggttgtcg cataattcac gaccatgtcg taggcgtcgt ggcggtgccc
    20941 ggcctcgacc atctggtgag cgcggatcag atccattgca tcgggcgaga gatgatagtg
    21001 gagaaccaag gggtgctgtt gaacgatggc ttcaagctct tcggcggtct tcggtttcga
    21061 cggttcggcc aattctttgc gatctttgac actcccttcg acgaacacag tcagagctgc
    21121 ggcaccgata acccagtcgc gatgagacga cacgcggtcg agccaggccc ggtagcggct
    21181 ggcagccggc aagagcgaga tgcggtcaaa cgtgggcggc ggcaggccta agccggccat
    21241 catggtcaga aacaactcgg gatgggattt gcccagcgaa agtccgccgg tgtcttcttc
    21301 gtaaatattc tctgcgagca tgcggcggac cgtagagggt ggattgtggc cgtggatgcg
    21361 tgccaggagc accggaaaat cccgcacgta cacgccatac tcctgctgaa aatgtcgttt
    21421 caattgctcg atggtgaccg ttccgtcggc gaacgccggc caggcccaat ggtgcttttg
    21481 atccatgatg cgaagaattt cttcctgaaa gcggcttttg gtgagcggtc gcgtcatgct
    21541 ggttgccttc ctgatctggg cctcgttaca atccgagcgc ggaggcggtt catgaaacaa
    21601 gtgacggtgc agactcccga ggacatactt tcattgctgg ccgatgttgc ccttcgcggt
    21661 tcgggcttcg tcacggagtg tctgctcgat tacgtgttgg acgaaggatt taccgagccg
    21721 atctatctga atgccagcgg agaagatccc gatgcctatt ataagggaca atcaccggcg
    21781 tgggcggtct accaggttcg tgaatggaaa cgcgtgctga caatttccgg agggcctggc
    21841 aaacagcggc gcgtccaaat caccgagacg ccatgacctg ttagagtgta tgagtcttca
    21901 gatgaaaaag caatgaaacg gatgagattt gcttcgctcc gattcgtcga aagaggggga
    21961 ctctagctcg tgagcttcgt gcatctggca gagccgtaca atctgggtga gctcgtcgtc
    22021 ctgcagagcc tgctcgaagg aagcggaatc gagtacgtga ttcggcatgc gcatatgggc
    22081 agcctctatc ccggggttcc cggaatgaat tcgcacgtca tggttgacga gcacgattat
    22141 tcccgtgcag aactgctctt gcagcgattg cggcttgatg tgcgcgaggt ctcagaagaa
    22201 gccgaggatt cgcgttagcg ttcgggtttg gggctgtgcg gatttgacgg agcttgtgtc
    22261 gggaggcgct cccctgagcc ttgaaaccat cctccgatga gtgtgccttc ttcaaagtac
    22321 agatagcggt gtttcaccac tgaagtgctt tcccagtctt caaagatcca ttcttcctgc
    22381 cgaatgccgt ctttcgtaaa tgtggtgttg atcgtttgtg gtccccccca ggcctgcagg
    22441 acctgctctt tcgacatgcc gaccatgacc cggtgttccg caatgtgctt ttgaaaattc
    22501 gggtctcgca tttgcgtgac caatggccag atgaccatca agagaaggaa gaggatcaag
    22561 ccggcgtata tcgctagacg gaccggtcgc aggcgaagga acgaaaccgg ttcctcagct
    22621 gaggcggcag gatttgcagg ggcagacgtg agttgttcca tgatattcct tcgcgcatcg
    22681 taacaacctc tcctagaggc cgtcaagctt cggcggacga attatcctga tgaatcaata
    22741 agatccgaat catgcgctat acttactgtt atgaggattt cgcttacgca gagtttcagg
    22801 cttcggtggt cgaattgtct cgccggcttc ttgctgtccg ggatcagttt gctgctcctc
    22861 gacgatgtgc tgtgtgccaa gctcctgtat tcctatcgcg atgaccaagg cacgaatgtg
    22921 atcacggata gctacgatcg cattcccatg cagtatcgtg caagagtcat cactgttgac
    22981 caagaatccg attcgctgaa ccaatcgggg aaaggagtcg cggacctact caaaggggcc
    23041 gacaagcgaa tcgggggggc gacgatcgat gtgcctggcc tctcccccta tcagtcgcac
    23101 gccttgacca ttacaggttc gctggcattg ctctgtttca tgcttcgtca gtttagccgc
    23161 agtcaggttc ttcggttcct ctctctttgg ggtctagtga tgctcgggct tgtcacacct
    23221 gtgttcatct acttgtcgca ggatggtccg ctcgacgtcc tccgcggagg ggcgtcgaaa
    23281 atccaaacca agcagctgga acatctgaag gacacgcggt agggctctcc cgctcgcaag
    23341 ctctccctac attcagtcag catacttcgg cagggggatt ttcttggcct gagccggtcg
    23401 gggaacagtc tgctgttggg agaactcatc cgaatagggg ttcatgatgg gatcgtgtgt
    23461 gtgcctctga cgccgcttca cgaggtcgat gctctgggtg ctgatttcca ttcgatcgac
    23521 gagcgatctg gtcgtgaggg gatcggggag accctgaaga gaaaacaatt ggtaggccag
    23581 cgtccagtcg agcgattcct tgccggattc cttgacgatg aagcgtgctt ctggtgagga
    23641 gcttcctttg aagagcagca cccatatgtt ggaacgaaac gagggagaag ccggttcctt
    23701 ggatgggcga aactccggga ccagcgaggg gacttggctg aacgggacgg cgggggaaaa
    23761 ttcgatatcc accagccgca caaagagcgg gcgattctcg ctttggtcgc tgggatcgat
    23821 cgcatagctg aaagagtagc gaacctcgat gccgtctcgc ttgtacgtat agacatcctc
    23881 tccgttttcg ggtgaaacca acttcctgaa atacttgtcc cagtcggtaa ttgcataata
    23941 cgtgaagacg cgcaaagccg acttgcggtc gcgaacggct tgtggcatgc ctaacttgtc
    24001 gtgaagctcg gtttgcgtaa ggccgagaaa tccgtcttca aaataggggg cggcgaagag
    24061 cgaacccggc agaaggcaga agtgagctag cagagcaaca atgcagaaac ggaagacgtg
    24121 ctgtgaatgg cgatgcagga gcaaggtgat tctccagccg ttgggccatc gtatcgacgg
    24181 ttcccctttc tgtcaaggta gcggggatga gatcggttgc cggtggtggg acttatcagt
    24241 atgatggcca tattattatt gggctgatcc tatggaggaa taaatcaggt gaccgcgttg
    24301 gaatcattac ttcgagaacg cattgtgatc ctcgacgggg ctatgggaac catgatccag
    24361 cagcgcaagc tggatgaggc ggctttccgc ggtgaacggt tcaaggattg gaaaaaagat
    24421 ctgaaggggc ataacgacct gctgaatctg acgcagccgg cgatcattga ggatattcat
    24481 cgccagtatc tcgaggccgg cgccgacgtt atcgaaacca acacattcaa ttctcaggcc
    24541 atctcccttg cggattacga gatggagacc ctggggtacg aaatgtccaa ggccggggcg
    24601 gaatgtgcgc gccgtgcggt caacgccgtg caggttgctc aggctggtcg gcgctgtttt
    24661 gtcgccggcg caatcggccc gaccacgaaa acgtcctcca tctccactga tgtcaataat
    24721 cctggtgcgc gcgggacgac ctacgaagag cttgtggcgg cctacagcga tcaggtccga
    24781 ggcctcctcg acggaggggt cgatgccctg cttgtcgaaa cgatattcga cacgctcaat
    24841 gcgaaagcgg cgttctttgc gatccaacag gcttttgcca acggcgggcg ccgagtgccg
    24901 atcatggctt cagtcacctt cattcaagcg gggagcaacc ggggtgtgac aggacaaacg
    24961 gttgaggctt tttggaattc catctctcac gtgcctctgt tgagcgtggg gatgaactgc
    25021 gcattgggcc ccaaggaaat gcggccgttg atcggcgaac tctcgcgcat cgctccgatc
    25081 tacgtcagtg cccatcccaa cgccggattg cccaatccgc tgctgccgac ggggtttcct
    25141 gagac
//
LOCUS       DATIFR010000053        11395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_416894, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000053 DATIFR010000000
VERSION     DATIFR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416894"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VLD60_03140"
     CDS             complement(<1..744)
                     /locus_tag="VLD60_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB43976.1"
                     /translation="MIDVQQITKRYGQHTAIDRVTFSVAKGEVLAFLGPNGAGKTTTM
                     RILTGFMPPTEGTARVAGFDCADQSLEVKRRIGYLPETPPVYQELTVSEYLQFVGRLR
                     GVAGNALKTAMDREVERLGLGSVQHRLIGNLSRGYRQRVGLAQALLHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSQSVILSTHILPEATAVCQRVVIISGGRIVAEDTPDQL
                     SARLRHSEKLSLTLLRPPQDAAALLGQISG"
     gene            1549..2184
                     /locus_tag="VLD60_03145"
     CDS             1549..2184
                     /locus_tag="VLD60_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSB43977.1"
                     /translation="MAYHTTQSSRSLLFLLVACAGALLGACSWIPKGEAQFDVGMKDR
                     GVASWYGEQFHGKQAANGEIFDMEGLTAAHRTMPLGSMVRVVNLSNGKHLHVRITDRG
                     PYVNGRILDLSRAAATQLGMERGGLAIVQVEIVGERRPEQLLSSDAELNRGSPYILGF
                     SALPEQEAPIMTPPLRALPSDLWMARRIRRVSAMLAADHTAHTEVAALALS"
     gene            2317..2535
                     /locus_tag="VLD60_03150"
     CDS             2317..2535
                     /locus_tag="VLD60_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43978.1"
                     /translation="MAKKKTTEPDELRLKKKIAAKLAGHSNPEGDAAMRALRKRLKRE
                     QRKRRALALRKKHAAGSKPAEVAAPAAG"
     gene            2597..3427
                     /locus_tag="VLD60_03155"
     CDS             2597..3427
                     /locus_tag="VLD60_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB43979.1"
                     /translation="MEEPRKEQDQKTPPGSDIATDELTDQVAAELTGNLAPAAEIPGE
                     QAEPVMLEEEKVKDEIDIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLCNALRDGD
                     WQVREVAIDALGQIGSPAVEMLIKLLRDWDVRKYAIIALGKIRDERVLDPLMVQLRND
                     EFKDDATNALVDLGEPALERLIKALKDKDELVRKQAVLALGRIKHADAIEPLIEMLAD
                     KDWFTRLTAAAALEAIGDDRGRNAIKPLMKDPDMVVRMRVERILAKWKKQPAESASTN
                     "
     gene            complement(3432..3887)
                     /locus_tag="VLD60_03160"
     CDS             complement(3432..3887)
                     /locus_tag="VLD60_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43980.1"
                     /translation="MWKFVGIVALALASFGLGYYFGQRPVGTLEQTVGDLRQSLKQMS
                     RDVLDTTMGIERDLRRRQALLDTKARVVQAKSDLFDKNYGEAAKQLAEGVRALESALK
                     GAKKDEATQALKTVAERLQEIRLELSMGKTIPMKKLDELQQEIDRQLNK"
     gene            complement(3890..5248)
                     /locus_tag="VLD60_03165"
     CDS             complement(3890..5248)
                     /locus_tag="VLD60_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB43981.1"
                     /translation="MLIFLLCSLVAFGFSNAADAATSSTATTLEREASEAFNKAEYGS
                     VLKLWQSLPPETTPSKMLIRLAFQSTLKLGRPEEGLAIYERAVKNDQPDDLAWLRPLA
                     ISFLSAYVRDPQEYIRIAAYTALAELGLPETRSLLEDGLLDASPLVRARAAEGIGRAG
                     LAGKSGSLRRALQDDMLPVRIAAMTALSEANVADIMPRLVEVARTEDGPEAVFAYAAL
                     YRLGKQDMLVDITGAATLPDPETRMAALGVLGRLKRPSSLAVLSQGVYDPEASVRAFA
                     AGALGEFGQPGAVAPLTHALGDENARVRGVAAASLGRIGIRDNRPLLQALTRDASMQV
                     RASAVEGLLRLGDASAVLIATDLARHPDPSVRAAAAQALGAISDKQAIVVLQTLLQDQ
                     QPQPRLFAAKALGKSTVPVLALLKKGLHDSDPAVRLTSAGSLLQQIDRLTKPSQRRGQ
                     KG"
     gene            complement(5311..7470)
                     /gene="glgP"
                     /locus_tag="VLD60_03170"
     CDS             complement(5311..7470)
                     /gene="glgP"
                     /locus_tag="VLD60_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSB43982.1"
                     /translation="MDTTPPITAPSNRIPASLVRLAELSQNLWWSWTLEARQLFEAID
                     PTLWSLTHHNPVKLLTDVNPERLAKLAEDPSYLRHYSAVLKMFDEYRANGRSWFSTKH
                     GTEVKSVIAYFSAEFGLHTSIPIYSGGLGILAGDHCKEASDLGVPLVGIGFMYPQGYF
                     KQRITAEGWQEAAYAPFNREDSPIHQALTPSGEHCRFNVDIGARNVTAVVWKVLVGRV
                     PLYLIDSDIPENSPENRALSARLYGGDQEMRLCQEILLGIGGVRMLRALGIAPSVWHA
                     NEGHSAFLTLERIRELVQKGLSHAEASELVRQSTVFTTHTPVPAGHDVFPHHLMDRYF
                     GGFWEQLGLSRDEFLQLGETPESHGQGFNMTALVMRLSAHVNGVSREHGRVSREMWKH
                     FWPGLPVEQVPIRSVTNGIHAPTWIAPELHRLYSKSLSPVWTETCDDQAMWQRVLEIP
                     DQELWAVRQALKRKLMAFIRERARTGWMHGHIQPSQVLSRGTLLDPEALTIGFARRFA
                     TYKRATLLFQDFERLNRLLQDKWRPVQLIFAGKAHPADEPGRYFIHEVLSFCHDHKLG
                     GHVAFLEDYDMHMAKYLVQGVDIWLNTPRFPMEASGTSGMKAALNGALHFSTLDGWWQ
                     EGYNGANGWGIQPLTGNVEVQAQDHHDGEQLYRMLEQEAVPLFYQRDLDGVPRGWLQL
                     VKESIRTVAPQFCTARMVKDYVETLYRPAMTRTPSTW"
     gene            complement(7539..8387)
                     /locus_tag="VLD60_03175"
     CDS             complement(7539..8387)
                     /locus_tag="VLD60_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43983.1"
                     /translation="MATTKTPYNQQLRSLAELMLAVAGESVTVMKKNAPAQALKLKRQ
                     DEWGLYLEFIKIMFNLTDRLSSFHIPIKDQPEFMNGLEDAVTSQLKTVLEPAFGSSVD
                     QTEIMLTIGGAVAESRQLYERYRFFINEESKTKHEMFEAFGGRVADALGVAGNLQITS
                     AATLCATAIVPAITAILKGDAPPAPAGTLAPSSEKAPATSSPAAPRGQTGSEIKLISV
                     MSNVTGEEVETRWGLHPRFRQDLSPEEVQQLTKLMNRVAKVLGERYATVAFSDEWNSW
                     HKAGHA"
     gene            8533..10560
                     /locus_tag="VLD60_03180"
     CDS             8533..10560
                     /locus_tag="VLD60_03180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSB43984.1"
                     /translation="MTREATPYILKRASDDLAPRKLAIDYAAALNPQQLAAVTAGDGP
                     ALVIAGAGSGKTRTLVYRVAYLIDSGVDPSHILLLTFTRKSAQEMLDRAGELIGARSE
                     RVCGGTFHSVANMLLRRYGRAIGLEPSFTILDRGDAEDLIALVRSQMGLNEKDKRFPR
                     KGTIAEIFSKSENMLRSLDDIVVEEFSHFADHLDALCQLQKGYQAAKRQRQLVDYDDL
                     LVLLRRLLADDQQARQAISRLYRYILVDEYQDTNRLQADVIRNLAATHQNVMVVGDDS
                     QSIYAFRGATFKNIMEFPTLFPGATIYKLEENYRSTQPILNLANCIIDEASEKYSKRL
                     FTRKLDGPLPTLAEAAGENAQSRFIAQKILELREEGVPLTEVAVLFRSSFHSFDLEIE
                     LSRKGLPFIKRGGVKFIETAHVKDLLAHLRVVANPLDAVSWHRVLMLVEGVGPKKAQD
                     LLATLMKSERPYQALREVTGRSGQGLKNLANTLESLSGIDEPGTADQVNHIYEYYLPI
                     LKEQYDDYPKRIRDLDHLHTIAEGYPEVDSFLADLALEPPDGSAVDVEAPDRDDERMV
                     LSTIHSAKGLEWQCVFVIWVVDGKFPSIYSFVTDEELDEERRLFYVAVTRAKRHLFLT
                     YPINVFDKSSGMLLSKPTRFLDHVSPAMFEQLALIEEGSHAEWRGYRDDSY"
     gene            10620..>11395
                     /locus_tag="VLD60_03185"
     CDS             10620..>11395
                     /locus_tag="VLD60_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43985.1"
                     /translation="MTRTSLRFLIAAALLVSLMLSPAGGDGLEAPQVSGHIWKQLVVE
                     AEGLGLPTKFLKAVPPDFVKFEFDDLQAFAAEYHLGEHRMVLNRTLSFNAAGGALRSL
                     SRLTHAEMETLYHELFHANMDYLMTVWEENQPDPLLTFARLQQRCRYSAVLITPVVQR
                     RTETEERFLSEREAWEALNEAWAVFIGWSVWNQLELGQRMGRSIQKPGKSRETWLRRL
                     DEADRDGKLRGYYEPEDFAERAVTRKRFLAPSSRLSVEEAT"
BASE COUNT         2337 a         3081 c         3515 g         2462 t
ORIGIN      
        1 gcccgagatc tgcccgagga gtgccgcagc atcttgcggt gggcgtaaga gtgtgaggga
       61 cagcttttca gaatggcgca gccgcgccga gagttgatcc ggcgtatctt cggcgacgat
      121 acgtccgcca ctgatgatga ccacacgctg gcagacggcc gtggcctccg gcaaaatatg
      181 tgtgcttaag ataaccgact gggaaccggc cagactcttg atcaattccc ggatttcgat
      241 gatctgcttc ggatcaagcc ccaccgtcgg ttcgtccaga atcaggaccg gtggatcgtg
      301 gagcagcgcc tgcgcgagtc ccacgcgttg ccgatagccc cgcgacaggt tgccgataag
      361 ccggtgctgt acggatccca gcccgagccg ctcgacttca cggtccatgg ccgtcttcag
      421 ggcgtttcct gccacgccgc gaagtctgcc gacaaactgg agatattccg atacggtcag
      481 ttcttggtag accggcgggg tttcaggcag gtagccgata cggcgtttca cttcgagcga
      541 ctgatcggcg cagtcgaacc cggccacccg tgccgttccc tcagtgggcg gcataaagcc
      601 ggtgagaatg cgcatggtgg tcgtctttcc cgctccgttg ggaccgagaa atgccaagac
      661 ctcgcccttg gcaacggaga aggtgacgcg gtcgattgcg gtgtgttgcc cgtagcgttt
      721 cgtgatctgt tgaacatcaa tcatagatag caggcatgcg tgccggtcga atggatgacg
      781 tcggggatta caagggcggg ttggtcatcg ccatctgatg cacaactgtt cggcatctta
      841 ctcaggcggc aggaggcagt caatatcgag tcgttgtccg gtcacaacct ttacatgatc
      901 cgggcatcct gctagagtcc tgaccgtctg ggcggacaga cggatgtaac gacgtatcgt
      961 gatgcggcac cactactggt taacggcctt tgttgcggtt ttgtcttttt ccctatcctc
     1021 tctcgcaccc ccttcgagcg aagcaagaca ggcacggacc tacaaggttc aaggtacagt
     1081 gattgctgtg acattaagcc aagaccctcc tttgattgtt gtaaggaccc ccctcggtcc
     1141 ccggaaccat atgacggtgg gagcgacggt gacggctcag actaagatac tccggggcaa
     1201 caaacgggtg gcgctgaata cgatcaaaga gggggaatct gtttggctga cttatgcaaa
     1261 aacgccaagg ggcgtcttgg ccgaaatgat ccgagtcgga ggttgaggct cagtcagggc
     1321 ggttgacgcg gtcggtccta tccatatata tatagactag ttagtccagt gctcaccggt
     1381 gtatttgtgt tcagttccac ccggcgactc tcctttttta tccagccaaa ggtaggggta
     1441 ggggctacag cttcggtatt gttgaacggc ccgtccgggc ttagcttgaa cgtgacaggc
     1501 gagctttacc ggctctgtca gttcaaccaa gtaacacagg tgttgaaaat ggcttaccac
     1561 acaacgcagt ccagccgatc gctcctgttt ctccttgtag cctgtgccgg ggccttgtta
     1621 ggggcttgtt cctggattcc caaaggcgag gctcaattcg atgtaggcat gaaggatcgc
     1681 ggggttgcgt cctggtacgg tgaacagttc catggaaaac aggccgcgaa cggggaaatt
     1741 ttcgatatgg agggcctgac tgcagcccat cgaaccatgc cacttggcag tatggtccga
     1801 gttgtcaatt tgagcaatgg caagcatctc cacgtgcgga ttaccgaccg ggggccatac
     1861 gtgaatggcc gaattcttga cctatcccgt gccgcagcca cccagctggg aatggagcgg
     1921 ggaggcctgg cgatcgtcca agttgaaatt gttggagaac gccgcccaga acagctcttg
     1981 tcgtcggatg cggaactaaa ccggggctct ccttatatac tcggattcag tgctttacct
     2041 gaacaggaag cgccgatcat gacccctccg cttcgcgccc tcccgagcga tctctggatg
     2101 gctagacgaa ttcgccgcgt ttcagccatg ctagctgcgg accatactgc gcataccgaa
     2161 gtcgcagctc tggccttgag ttaggtccta ctcgtcttct ccgatcaggt tgacactgtg
     2221 caaagctctc gattagactg ccctgtccgg ccactcccag atggagcggt cggatattga
     2281 ttctgagggg cggtatacat ggggggcgga aggtcgatgg caaagaaaaa aacgacggaa
     2341 ccggatgagt tacgattgaa gaaaaagatt gcagcaaaac tcgcaggaca cagcaatcct
     2401 gaaggggatg cagcgatgcg cgcgctgcgc aagcggctca agcgtgagca gcggaagcga
     2461 cgggctttgg cgctccggaa gaagcatgct gcaggcagca agccggccga ggttgcagct
     2521 ccggcggcag gataatcaag attttgcaga tgaccagatg acattggtcg agcgcgcgtc
     2581 ataaggaata ttgaccatgg aagaaccacg caaggaacag gatcagaaga ccccccccgg
     2641 ctcggacatt gcgaccgatg aattgaccga ccaggtcgcg gcggagttga cgggcaatct
     2701 tgctcctgcg gcggaaattc cgggggagca ggccgaaccg gtcatgctcg aagaggaaaa
     2761 ggtcaaggac gagatcgaca ttcagatcga tctcctcaaa gaccccgact gggtggtgcg
     2821 tcgtgaagcg gcgattacgc ttggtgaaat gggtgacgag cgttgcgtcg aacccctctg
     2881 caatgcgtta cgcgacgggg attggcaggt acgcgaagtc gccatcgatg cgctgggtca
     2941 gatcggctct cctgccgttg agatgttgat caagttgctg cgcgattggg atgtcagaaa
     3001 gtatgcgatc atcgcattgg gaaaaatcag ggatgagcgc gtcctcgacc cgctcatggt
     3061 gcaactgagg aacgatgagt tcaaggatga tgcgaccaac gccttggtgg atttgggcga
     3121 gccggcgctc gagcgcctga tcaaggccct gaaggacaaa gatgaactgg ttcgtaagca
     3181 ggcggtgctg gcactcggga ggatcaagca cgctgacgcc atcgaaccgt tgatcgaaat
     3241 gctggccgat aaggattggt tcacccggct gaccgctgcc gcggcgcttg aggcgatcgg
     3301 tgatgaccgc ggacgcaacg ccatcaagcc gttgatgaag gaccctgata tggtggtgcg
     3361 gatgcgggtg gagcggattc tggccaagtg gaagaaacag ccggccgagt cggcctcgac
     3421 gaactagtcg gctacttgtt caactgccgg tctatttcct gctgtaattc gtcgagcttc
     3481 ttcatcggaa tcgtttttcc catcgacagt tcgagcctga tttcttgcag cctctccgcg
     3541 acggttttca aggcctgcgt cgcctcatct ttcttagctc ctttcagcgc gctttctaat
     3601 gccctgacgc cctcggccaa ctgcttggct gcctcgccgt aattcttgtc aaagagatcc
     3661 gacttcgctt gcaccacccg ggcctttgtg tccaagaggg cctgccgtcg tcgcagatcg
     3721 cgctcgattc ccatcgtcgt atccaagacg tcacgagaca tttgtttgag tgactgccga
     3781 aggtctccga ccgtttgctc cagtgtgccg acagggcgct gcccgaagta atatccgagt
     3841 ccgaacgagg cgagggctaa ggccacgatg ccgacgaatt tccacatgac tatcctttct
     3901 gtccccgccg ctgcgagggc ttcgtgagac ggtcgatctg ttgaaggagg cttccagccg
     3961 acgtcaggcg aacggctggg tcggaatcat gtaagccttt ttttagcaat gccagtaccg
     4021 ggactgtgct tttccccaac gccttggccg cgaacagtcg cggttgcggc tgctggtcct
     4081 gtaacaatgt ttggagcacg acgatggctt gcttgtcgga gatggcaccc agcgcttgtg
     4141 ccgctgcagc gcgaacggag ggatccggat gccgcgccag atccgtcgca atgaggacgg
     4201 ccgaggcgtc tcccagtcgt agaagccctt cgacggcgct ggcgcgtacc tgcatcgatg
     4261 catcgcgcgt cagggcttgc aggagcgggc gattgtctcg aatgccaata cgtccgaggc
     4321 tagcggctgc cacgcctcgg acgcgggcat tctcgtcacc gagcgcatgg gtgaggggtg
     4381 caacggctcc cggctggccg aactccccca acgctccggc tgcaaacgcg cgtaccgaag
     4441 cttccgggtc gtagacgcct tggctgagaa ccgccaggct tgatggacgt ttgagccgcc
     4501 cgagcactcc gagcgcagcc atacgtgttt cgggatcggg aagcgtggcg gctccggtaa
     4561 tatctacgag catgtcttgc ttgccaagcc tgtagagggc ggcgtaggca aagacggctt
     4621 ccggtccatc ttccgttctg gcaacttcga ccaatcgcgg catgatgtcg gcgacgttgg
     4681 cctcgctcaa ggcggtcatc gcggcgatcc gcacgggtag catgtcgtct tgcagcgcac
     4741 gccgcaaaga gccggacttt cctgcaagcc ctgcacggcc gattccctcg gcagcacggg
     4801 cgcgaacgag cggcgacgca tccagcaagc catcctccag gagagatcgg gtctccggca
     4861 atccgagctc ggctagcgcg gtatacgcgg cgatacggat atattcctgg ggatcacgca
     4921 cataggcgct caggaaactg atggcgagtg ggcgcaacca ggcgagatcg tccggctggt
     4981 cgttcttgac ggcgcgctca tagatagcca gcccttcttc aggccggccc agcttgagcg
     5041 tactctgaaa ggccagccgt atgagcattt tcgatggcgt tgtctccggc ggcagcgatt
     5101 gccaaagctt aagaacactg ccgtactccg ctttgttgaa ggcctcggag gcttcgcgct
     5161 caagggtggt ggcggtcgag gaggtcgcgg catcggccgc gttcgagaag ccgaaggcga
     5221 ccagtgagca gagcaggaag atcagcatgg agggagtgat aacatcgaac ggccaagact
     5281 gtcgagagaa cgtcagccgg agcactctcc ttaccatgtg gaaggagtgc gcgtcatcgc
     5341 gggacgatac aacgtctcga cataatcctt gaccatgcgc gcggtgcaga actgcggtgc
     5401 gacggtccgg atgctttctt tcaccagttg gagccagcct cgcgggactc cgtcgagatc
     5461 gcgctgataa aacaacggaa cggcttcctg ttccagcatg cggtagagtt gttcgccatc
     5521 gtggtgatcc tgagcctgca cctcgacgtt cccggtcaag ggctggatac cccatccgtt
     5581 cgccccgttg tagccttcct gccaccagcc gtcgagcgta ctgaaatgca gggctccatt
     5641 gagggcggcc ttcattccgc tggtgccact ggcctccatc gggaaacgcg gtgtgttcaa
     5701 ccagatatcc acgccttgta caaggtactt cgccatgtgc atgtcgtagt cttcaagaaa
     5761 ggccacgtgg ccgccgagct tgtggtcgtg acaaaaactc aggacctcat gaatgaaata
     5821 acggcctggt tcgtcggccg ggtgggcctt cccggcaaaa attaattgaa ccggtcgcca
     5881 cttgtcctgc agcagccgat tgagccgctc gaaatcctgg aacaagaggg ttgcccgttt
     5941 ataggtggcg aagcgtctgg cgaagccgat ggtcagggcc tcggggtcta agagcgtccc
     6001 gcgagagaga acttgtgagg gctggatgtg gccgtgcatc cagccggtgc gggcccgttc
     6061 gcggatgaag gccatcagct tgcgtttcaa tgcctggcgg accgcccaga gttcttggtc
     6121 ggggatttcc agcacgcgtt gccacatcgc ttgatcatca caggtttcgg tccagaccgg
     6181 gctgagcgac ttactataca gacgatggag ttccggagcg atccatgttg gtgcgtggat
     6241 gccgtttgtg acgctccgga tcgggacctg ttcgaccgga aggcccggcc aaaagtgttt
     6301 ccacatctcc cggctcacgc gtccgtgctc ccggctcacc ccgttgacgt gtgccgagag
     6361 gcgcatgacg agcgccgtca tattgaaacc ctgcccgtgc gactccggcg tctcgcccag
     6421 ttgaaggaac tcgtcgcggg agaggccgag ttgttcccag aaaccgccga aataccgatc
     6481 catcagatgg tgtggaaaca cgtcgtggcc ggccgggacc ggcgtgtgag tcgtgaatac
     6541 cgtgctttga cggacgagct cgctcgcctc ggcatgagac aaacccttct gcacgagttc
     6601 gcggatgcgt tccagcgtga ggaaggcgga gtggccctca ttcgcgtgcc agacggaggg
     6661 cgcaatgccc aacgcgcgca gcatgcggac cccgccgatg cccaagagga tttcctggca
     6721 gagtcgcatt tcctggtcgc caccgtagag acgtgccgac agggctcgat tttcagggct
     6781 attctccgga atgtcggaat caatcaagta gagtggcacg cgaccgacga gcactttcca
     6841 gaccacggcg gtgacgttcc gtgcgcctat atcgacgttg aatcggcagt gctcgccgga
     6901 cggtgtcagt gcctggtgga tcggggagtc ttcgcgattg aacggagcat aggccgcttc
     6961 ttgccagcct tccgcggtga tgcgctgctt gaaataccct tgcggataca tgaacccgat
     7021 gccgacaagc gggacgccaa gatcgctggc ttccttgcaa tggtcaccgg ccaaaatgcc
     7081 aagaccgcca ctatatatgg ggatggaggt atgcaggccg aattcggcgg aaaagtaggc
     7141 gatcacgctc ttgacctctg tcccatgctt tgtgctgaac cagctgcgtc cgttggcacg
     7201 gtattcgtca aacattttga gcacggctga gtagtgcctg agataggaag gatcttccgc
     7261 gagcttcgcc aaccgctccg ggttcacgtc ggtcaggagt ttgacaggat tgtggtgtgt
     7321 cagggaccag agcgtggggt cgatcgcttc gaacagctgg cgtgcttcca gcgtccagct
     7381 ccaccaaagg ttctgagaga gttctgccag ccggacgagg ctggctggga tgcggttcga
     7441 cggtgcagtg atcggcggag tcgtatccac tgggtctcct tagtggtgga gtcgatgtcg
     7501 gcgagaagag atggcctgat cgctctgtga aggtcgattc aggcgtggcc agccttatgc
     7561 caagaattcc actcatcaga gaatgccacg gtggcgtacc gttcgccaag cactttcgca
     7621 actcgattca tgagtttcgt gagttgttga acttcttcgg ggctgaggtc ttggcggaaa
     7681 cgcggatgga gtccccagcg cgtttcgact tcttcgccgg tgacgttcga catcacgctg
     7741 atgagtttga tctcgcttcc ggtttgacct cgtggcgcgg caggcgagct cgtcgcaggc
     7801 gctttctcgg aggatggtgc caaagtgccg gccggagccg gcggcgcatc ccccttgagg
     7861 atggcagtaa tcgccggcac gatggccgtg gcgcaaagtg tcgcggccga ggtgatttgc
     7921 agatttcctg ccacgcccaa ggcatcggcg acgcgcccgc cgaacgcctc gaacatttcg
     7981 tgtttggttt tgctctcttc gttgatgaaa aagcgatagc gctcgtagag ctggcggctt
     8041 tccgcgacgg ctccgccgat cgtgagcatg atctccgtct ggtcgacgct ggaaccgaag
     8101 gccggttcca gtacggtctt cagttgagaa gtcaccgcat cctccaggcc gttcatgaac
     8161 tccggctggt ccttgatcgg gatatgaaag cttgagaggc gatcggtcag attgaacatg
     8221 atcttgatga actcaagata caggccccat tcgtcctgac gtttcagctt gagtgcctgc
     8281 gcaggggcgt tcttcttcat gacggtgacc gattcgcctg caacggcaag catgagttct
     8341 gcgagcgagc gcagctgttg gttgtagggt gtcttcgtcg tggccataag tctctgtagt
     8401 gctccagtga attcgaaggc ccattatagc tgagaaatta tccggcctcc aagtgggcag
     8461 tatgacctgc gcggatcatt gaaaccccct cctctgtagt gctatacagc gtactcacct
     8521 cctatttgcc tcatgacccg agaagccaca ccctacattt tgaagcgtgc gtcggatgac
     8581 ctcgccccgc gcaagttggc tattgattat gcggcggctt tgaatcctca gcaattggca
     8641 gcggtgacgg cgggcgatgg gccagcattg gtgatcgccg gagccgggag cggcaagacc
     8701 cgtacgctcg tttatcgcgt ggcgtatttg atcgattcgg gggtcgatcc ctcacatatc
     8761 ctgctgctga cgttcacgcg aaaatcggcg caggaaatgc tcgatcgggc tggcgaatta
     8821 atcggtgcga ggagcgagcg tgtctgcgga gggacgttcc attccgtggc gaacatgttg
     8881 ctccggcggt atggccgagc gattggtttg gagccaagct ttaccatctt agaccgtggt
     8941 gacgcagaag atttgatcgc gcttgttcgc tcccaaatgg gattgaacga gaaagacaag
     9001 cgctttccgc gcaaaggaac aattgccgag atcttcagca agtccgagaa catgctgcgc
     9061 agcctggacg acatcgtggt ggaggagttc agccactttg ccgatcatct cgacgcgctc
     9121 tgccagctcc aaaagggcta tcaggccgcg aagcggcagc ggcagttggt ggactacgac
     9181 gatctgctcg tcttgctgag gcgcttgttg gcggatgacc aacaggcgag gcaggcgatc
     9241 tcgcggcttt atcgctacat ccttgtcgat gagtatcagg atacgaaccg gctgcaggca
     9301 gatgtgatcc ggaatctagc agcgacgcat caaaacgtga tggtggtggg cgacgacagc
     9361 cagtcgattt atgcatttcg cggcgcaaca tttaagaaca tcatggagtt tccgacgctg
     9421 tttcccggtg cgaccatcta taagctcgaa gagaactatc gcagcaccca gccgattctg
     9481 aacctggcga actgcatcat cgacgaggcg tcagaaaagt atagcaaacg gctctttacg
     9541 cggaagctcg acgggccgct gccgacgctc gccgaagcgg cgggtgaaaa tgcgcaatcg
     9601 cgcttcattg cgcagaagat tcttgaattg cgggaagaag gtgtgccgct gactgaggtg
     9661 gcggtattgt tccggtcgag tttccattcg ttcgatttgg aaattgagtt gtcccggaaa
     9721 gggctgccct ttatcaaacg gggcggggtc aagttcatcg agacggcgca cgtgaaggat
     9781 ctgctcgcgc atctgcgcgt tgtggctaat ccgttggatg cggtgagctg gcatcgggtt
     9841 ttgatgctcg tcgaaggtgt cggtccgaag aaggctcagg atcttcttgc aacactgatg
     9901 aaatcggaaa ggccgtatca ggcattgcgg gaagtaaccg gccgatcggg acaggggcta
     9961 aagaatctgg cgaacacgct tgaaagtttg tcgggtatcg acgagcctgg aacggctgat
    10021 caggtgaacc atatttatga atattacctg ccgatcctga aagagcagta cgacgactac
    10081 cccaaacgga tcagagatct tgatcacctc catacgattg ccgagggtta tccggaagtc
    10141 gacagttttc tcgccgacct ggcgctcgaa cctccggacg ggagcgcggt ggatgtggag
    10201 gccccggatc gcgacgatga acggatggtt ttatcgacga ttcattcggc aaagggcctg
    10261 gagtggcagt gtgtgtttgt aatttgggtg gtcgatggaa agtttccttc gatctactcg
    10321 ttcgtcaccg atgaagaact ggatgaggaa cgtcggctct tctatgtggc ggtaaccagg
    10381 gcgaaacgtc atctgtttct tacctatcca atcaatgtat tcgacaagag cagcggcatg
    10441 ctgctttcaa aacccacgcg ttttcttgat catgtttctc cagccatgtt cgagcagctg
    10501 gcgttgattg aagaagggag tcatgcggag tggagggggt accgggacga ctcctactga
    10561 tccgctggct tgcattctga gtcaccgggc tgcgacgatc tgatcattct gtttttccaa
    10621 tgacacgaac gtctttgcgc tttctcattg ccgccgcttt acttgtcagt ctgatgctat
    10681 ctcctgccgg cggagatggt ctcgaggctc cgcaggtatc tgggcacatc tggaaacaat
    10741 tggtggtcga ggctgaggga ctcggcctcc ccacgaaatt cttgaaagcg gtcccgccgg
    10801 acttcgtgaa gtttgagttc gacgatctac aagcgtttgc agctgaatat catcttggcg
    10861 agcatcgaat ggtgttgaac cggacgcttt cgttcaacgc ggcgggaggt gcgttgcgtt
    10921 cactcagccg gttaacccat gcggagatgg aaacgctgta ccatgaactc tttcatgcga
    10981 atatggacta tctcatgacg gtgtgggagg aaaatcaacc cgatccgctc ctcacgtttg
    11041 ccagattgca gcaacggtgc cgctacagtg ccgtgctgat cacgccggtc gtgcagcgga
    11101 gaacggagac agaagaacga tttctcagtg agcgtgaagc atgggaagcg ttgaacgaag
    11161 cctgggctgt cttcattggg tggtcagtgt ggaatcaact ggaacttggt caacggatgg
    11221 gacgttcgat tcagaagcct ggaaagagtc gcgagacctg gttgcgccga ttggacgagg
    11281 cggatcggga cgggaagctt cgaggttatt atgagccaga ggactttgct gaacgagctg
    11341 tgacgaggaa gcgttttctt gctccgagtt cgcgcctctc ggtggaggaa gcgac
//
LOCUS       DATIFR010000054         1817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2523843, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000054 DATIFR010000000
VERSION     DATIFR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523843"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..810
                     /locus_tag="VLD60_03190"
     CDS             454..810
                     /locus_tag="VLD60_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43986.1"
                     /translation="MVIVTLVMFGHAQAQDEPIPVDLKVGETFDICASGLIRCPPNDN
                     KCDDPKVAIPVTASDGSGGLVFQGVGPGTTLCSALSVSGSRAIYRVTVSPTPQGDAKD
                     NTLRLPAPKGRQKIMY"
     gene            complement(860..1099)
                     /locus_tag="VLD60_03195"
     CDS             complement(860..1099)
                     /locus_tag="VLD60_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB43987.1"
                     /translation="MFTEPCSCGVCVDINKANITGSAYNPETQETYLRHLCKCGEERL
                     TYSNGTPDAVQQRAIDADELMTEILEHHMERPPRW"
BASE COUNT          383 a          517 c          506 g          411 t
ORIGIN      
        1 ggaggataac gtcacgcaaa agcctcacag gctcctcgga gatattatgc tggaaaataa
       61 ctggataacg gaagaacaaa tggaatcaat attggatgaa atgggtgtgg gaaaacaggt
      121 ggtgggctga agctgcctcc gccgcctcct gccgctcgcc gcgcccgttg tggatcaatc
      181 acgcgatgct gcttcttctt caatcctcat attccttcca cctaccgacg gggcaaccac
      241 tcgggccgtc agggacgcta ggggaggtac tgaccgtccg atgcgagcct ttgagatttg
      301 ccccgccgat agccgcacgc cgggagcgtg accgatccaa acgccctaat cgtttggagc
      361 gtgtatttac tcgctcctag cggtactctt taaacatatc ctgtccaagc gaggtgggac
      421 atgcgggcaa gatctgttgt ggtgtgcatc ccaatggtta tcgtcaccct tgtaatgttc
      481 ggccacgccc aggcgcagga tgagcctatc ccggtcgatt tgaaggtcgg agagaccttc
      541 gacatctgcg catccgggct gatccgttgc ccgccgaatg acaacaaatg cgacgatccg
      601 aaggtcgcca tcccggtcac cgcttccgac gggtccggcg ggttggtatt ccagggagtc
      661 ggacccggga cgacgctctg ctcggcgctc tccgtcagcg gatctcgcgc catctaccgc
      721 gtcaccgtct ccccgacccc ccaaggcgac gcaaaagata acaccttgag actcccggcc
      781 cccaagggga ggcagaagat aatgtattga gactctcggc ctatccgggc gtcgtgagga
      841 ccgccccccg gctaccacgt taccaacgag gcggacgctc catgtggtgc tccaaaattt
      901 cggtcataag ttcgtccgcg tcaatcgccc gttgctgcac cgcatccggg gtgccgtttg
      961 aatatgtgag ccgctcctcg ccgcatttgc acaaatgccg gaggtacgtt tcctgcgtct
     1021 cggggttgta ggcggacccg gtgatgttcg ccttgttgat gtctacgcaa accccgcagg
     1081 agcacggctc cgtaaacatg ggctgtaccg gctccgggtt cgccacaaat cggccttgct
     1141 cgtcgcgtcc gcgctcgccg atctatcgct gagcgcccgc agaaggagac aaagatgagc
     1201 ttcacggtcg atctcgaaat acttggtgaa gacgaagtca gacagcgcct ccgaacgaga
     1261 ggcccgagag aaagaaagtt tatcgatctc tcgtaaagct ttttggatct cggtgtgcgc
     1321 aattatcgta gctattgcag cctcctattc agcattttga aaaaatgatc tacttggcgg
     1381 ctcgcatcgc cgagctatac cgcaattaag gaatggactg acctcttgaa caggagggag
     1441 atggccgtgt ggcggaggtt gtggatgtaa tctagggctt tcgggtgtac acaccgaagg
     1501 tcgaatccgt taactagtct ttactgataa agtatctttt cttcggccgt aaattgtgac
     1561 cgtcttgtga ctggattact ggtttcgttg ttggagcagg gtatgatcgt ttccgggacg
     1621 agtgggagat actgatgatg gaaccgcccc catccaccgg ttgatcaccc tgctggccag
     1681 ccccccgtgc cggcctttca ccttgacagc ccatacgttt gattcatatt gattcatatc
     1741 ttgattccat cccgtcctcg ggctcggacc accatgaacg gtgagagccg atatcgaagt
     1801 acttggcgat ccgatca
//
LOCUS       DATIFR010000055        36885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1375295, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000055 DATIFR010000000
VERSION     DATIFR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36885
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375295"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /gene="lpxB"
                     /locus_tag="VLD60_03200"
     CDS             complement(<1..873)
                     /gene="lpxB"
                     /locus_tag="VLD60_03200"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSB43988.1"
                     /translation="MRILIVTGEASGDLHGANLARAIWAIDPKAELVGIGGAAMRAAG
                     VILVPGVPHLDVMGLIGLSAIRAMVQRIRAIRRVLKSEAWDLVVLIDNPGLNFHFARV
                     AKAAGRRVLYYIAPQIWAWRPRRIKWIQRRVDHVVVILPFEPEIYRRAGVRCTFVGHP
                     LLDAVAPQYDRQRLRRTFDLEDSGPVVGLLPGSRVAEVTMLLPVLLETAAKLAAVEPK
                     TRFILAQASSIDDNLLQGLLHQSSVPVRVVRDQASEVMAAADVLLIASGTATLQAAVV
                     GTPMVLLYKTTPLTY"
     gene            complement(960..1922)
                     /locus_tag="VLD60_03205"
     CDS             complement(960..1922)
                     /locus_tag="VLD60_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB43989.1"
                     /translation="MTALKAGVIGVGHLGQHHARLYASLPGAQLVAVTDQSMDRAQLI
                     ADRHRVRATRSLGELLPLVEVVSVAVPTSAHYAVAKACLLAGKHVLVEKPIAVTSAQG
                     HELVQLARQQGRCLQVGHSERFNPVMQVMRPHIGRPVFIECHRLSSFSERGTDVDVVL
                     DLMIHDLDLVLSFNPGPVEDIQAAGVPVLSSTIDIANARIQFKGGCVANLTASRVSMN
                     KMRRLRVFQQDKYISIDFQSRQGVICRRDAKAGQRPAVDVEQLQGGEEEPLKLQLESF
                     LHAAGTGTRPVVSGEDGAAALDVAQQVLQAITAFMARQDEGTKG"
     gene            complement(1919..2782)
                     /gene="lpxI"
                     /locus_tag="VLD60_03210"
     CDS             complement(1919..2782)
                     /gene="lpxI"
                     /locus_tag="VLD60_03210"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HSB43990.1"
                     /translation="MTLAPQVRNGERLGLIAGNGRFPIIFAENARKLGYLVSAVAHEG
                     ETDPDLANHVDRIHWIKIGQLNKLIKAFKEDEVHQAVMLGGIKKTHVFTTVRPDFRTL
                     ALATRLALWKDDDILREFAKELEREGISICESTFGLEGILAEEGTLTARVPSQKEWED
                     IRYGWEVAHDIGRLDIGQCVVIKDRVVVAVEAVEGTDEAIKRGGQLAKEGAVVVKRCK
                     PQQDLRFDLPAVGPRTIDVMKSVKASVLAVEAGRTILLDRELMLEKACSAGIAVVGLV
                     KPDATGGTTAP"
     gene            complement(2788..3594)
                     /gene="lpxA"
                     /locus_tag="VLD60_03215"
     CDS             complement(2788..3594)
                     /gene="lpxA"
                     /locus_tag="VLD60_03215"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSB43991.1"
                     /translation="MQIHPHAIVHSAAQLADDVVVGPFCVVGEHVTIGKGTRLLSHVT
                     VEGWTQIGERCELHPFVSIGGPPQHLGYKGEPTKVIIGDDNIFREYVTVNRATVQGGG
                     ITSIGNKNFLMAYVHVAHDCRLGSHLIMANAASLAGHITIGDHAIIGGLTGIHQYVRV
                     GAYSMVGGCCALGQDLPPFMRAAGGYRARMYGLNSVGLRRHGFSSDRVAVLKRAYDIL
                     FRSGHRTAEAARLAKAEFGDQPDVMAVVAFLEGTKRGICRSVAKEQEDEE"
     gene            complement(3714..4157)
                     /gene="fabZ"
                     /locus_tag="VLD60_03220"
     CDS             complement(3714..4157)
                     /gene="fabZ"
                     /locus_tag="VLD60_03220"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HSB43992.1"
                     /translation="MSVMEQADIQALLPHRYPFLLVDRVLELDPDRRIVGIKNVTVNE
                     PFFQGHFPGRPVMPGVLILEAMAQVGGLLAFKSMGEAKRPVVYLTGVDGAKFRKPVVP
                     GDRLRFEVDVVKKRAPFWKMQGKAFVEDELVCEAEVTAMVTEEKA"
     gene            complement(4203..4739)
                     /locus_tag="VLD60_03225"
     CDS             complement(4203..4739)
                     /locus_tag="VLD60_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSB43993.1"
                     /translation="MRASKLVNLITVVTIWSAMLSAAFAADLSKVAVMDQQLVIEKSK
                     AGKRALEELKAYAATRQKIINADDQELKELEQAAQDSKLSETAKQEKQAQFQAKVEAY
                     QRRLADFNREIQQKQRETVAEYSKKVRDAAQVVAQREGAIAIIDKGNDAVIKIVIYHQ
                     PGLDMTEQVVKEFDRQNK"
     gene            complement(4780..5334)
                     /locus_tag="VLD60_03230"
     CDS             complement(4780..5334)
                     /locus_tag="VLD60_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSB43994.1"
                     /translation="MIQSQTVNKIFWLSIGLAFWVGSAMGCAGAGAKVEGKIGLVDPA
                     RLLNEANAGKKTKDSLAAFSKNRQALLEIEEKELRRMEEDFIKQASVLSPAAKSEREQ
                     AFRRRMAEYQQKAGELNREIQEKQKEAYEGFRDKVEVVVAKVAKRHGLQVVIDKGKGG
                     PAIFGDESLDITNQVIEEFNREYP"
     gene            complement(5360..7747)
                     /gene="bamA"
                     /locus_tag="VLD60_03235"
     CDS             complement(5360..7747)
                     /gene="bamA"
                     /locus_tag="VLD60_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HSB43995.1"
                     /translation="MSRLDDGFVMLSGHVWSADLVREVSSLQGSRWALVLLITLFYGA
                     VLACEAIAQPTAVKVGSIEIRGNKKIELQAIEGRLTLKPGDPYTPENVRGQIKILYDT
                     GYFEDVQVETEPIPNGMALTFLVREKPFITEIVFDGNTEMSDDKLKEKITIKSQAFLD
                     QQQAKESAEKIRLTYQEDGYFNCQVIPVVQTLDEDRKRLTFLVKEGDKARVKVVNFDG
                     MHAASKEEMFKVMATREWVPWYGIFTKLQLPSFLSDAGVLKREESNNDVERIKEVLLN
                     KGFLNVQVGLPSVELSSDRKWFVVTYAVVEGEPFTVAEVGFRGNTVFEDPELREKLKI
                     KPGEIFQRQKIRDEITRLTDLYGSKGYAFADVTPSVTPNPEERTATIILSIKEGEMMR
                     IRQININGNDKTKDNVIRREVRVDEQDVIDTPALKRSFQRLNNLNFFETVEILPQQVD
                     VDKVDLNVRVKEKPTGQFSIGGGFSTLDKLVAIADITEGNLGGNGWMGRIRGQLGQNR
                     TLGLITFRNPYLNDSLTSMQLDIYRSMTDYITYFEKKTGASITFGRWLSEYVSGSFSL
                     MAEQLDYSDPNNNCGFNPGLIICQQLGNQTTTGFRTSIARDTRDYYLDPRTGWRTSAG
                     FDLGTPYLGGSNNFYKYNFDVLKYTPLPYDTRFSIHARYGEANSINGEPVPLTELFFV
                     GGINTMRGFQFGRAGPVDPNTKTLLGAQKQLIFNNDFIFTISSEAKLNGVIFFDYGKG
                     FAQDESVSFDLRKAAGLEGRWISPFGPLRAAYGINLDAREGERKGVFEFTIGSLF"
     gene            8020..8925
                     /gene="galU"
                     /locus_tag="VLD60_03240"
     CDS             8020..8925
                     /gene="galU"
                     /locus_tag="VLD60_03240"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSB43996.1"
                     /translation="MSRTEVRKAVIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVV
                     EEAVASGIEDIIIVTGRGKRAIEDHFDRSVELEENLKGNGKAQVLSQIRHISNLANFC
                     YVRQSEALGLGHAVLCAQHLIGDEPFAIMLGDEIIDAPVPGLAQLIHVYKKRRGAVLG
                     IQEVPHQEVNRYGIISPDRIADGLYKVTNLVEKPVPADAPSNLAVIGRYVLPPEIFPI
                     LRKTRPGKNGEIQLTDALKELAKSSPMYALEVKGNRYDAGDKLGFLIATVEFGLKNPT
                     LGADFADYLRNRPKSSGEQRRRGRA"
     gene            8981..11476
                     /gene="lon"
                     /locus_tag="VLD60_03245"
     CDS             8981..11476
                     /gene="lon"
                     /locus_tag="VLD60_03245"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSB43997.1"
                     /translation="MSDSIEQDLTPPQNVEIPDQLPLLPVRDIVVFPYMVLPLFVGRE
                     MSIKAIEAALAGNRMIFLATQKALDVENPTPDDIHTIGTVGIIMRMLKLPDERIKILV
                     QGLSKAKVSGYIQTEPYYSVRIDKIEESKPVSSALESEAVMRTVKEQIERIVSLGKVL
                     IPDVMVVIENLEDPGRLADMIASNLGLKVEVTQAVLEITDPLKRLRQISEILAKEIDV
                     LSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEVAEFRKRVKDSKMP
                     EKVLKEAEKQLKRLEKMHPDTAESATVRTYLEWMVELPWSKKSKDNLDLKAAAKVLNE
                     DHYDLEKVKERILEYLAVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARALGREFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRMIQGMKQAGTNNPVFMLDEIDKVGMDFRGDPSA
                     ALLEVLDPEQNSTFTDHYLGVPFDLSEVMFITTANLIDPILPALRDRMEVIEIPGYTE
                     EEKLGIAQRYLIPRQLSEHGITEKHVRIAEPAIRQIITNYTREAGVRNLEREIANVMR
                     KVAKKVAEGKGLGFPVNPANLHKYLGVPKYLPESELEKDEIGVATGLAWTEAGGDVLY
                     IEATVMKGKGQLTLTGHLGDVMKESAQAALSYVRSREKTLGINPDMFSKQDLHIHVPA
                     GAIPKDGPSAGITMATAIASAMAQIPARRDLAMTGEITLRGRVLPIGGLKEKILAAKR
                     AQLTTVILPKRNKKDLDEIPKHILKGIQLVFADTMDDVMRVALRRTPRATRSTGRPQA
                     PQQTQKRVAKNHKSRRIDRTVHAQSAIMAHSHE"
     gene            11487..12539
                     /gene="thiL"
                     /locus_tag="VLD60_03250"
     CDS             11487..12539
                     /gene="thiL"
                     /locus_tag="VLD60_03250"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSB43998.1"
                     /translation="MASPARPKAISTIREFDLIRALQQHHGAGDSSILRGIGDDAAVI
                     APSQNCWQLMTTDLLTEGIHFDLRTAVWSDIGFRAAAANLSDIAAMGGTPQYLLVALA
                     IPRHGTGRHVHHLYSGMMKACRPYSVRLIGGDISASAQGWFISITLIGSVLPRRALLR
                     SGARVGDSLYVTGTIGDSRAGLELLQRSRGRKAAASLSETHRHFLIQRHLRPTARVEI
                     GRWLSTHHWATSAIDLSDGLSGDLRHICAESRVGADIDLSALPISPACTAYAESTGQG
                     PITLALAGGEDYELLFTVSPRHRTRFERMAAGQRVRMTKIGTIRPARSGIHGIPLDGQ
                     RQPLPVTSYEHFCSFK"
     gene            12558..13037
                     /locus_tag="VLD60_03255"
     CDS             12558..13037
                     /locus_tag="VLD60_03255"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HSB43999.1"
                     /translation="MASFRSILRQVLHLQESPQRTAVAFSIGAAIAFCPFYGLHMILV
                     VFCTWAFGLNFLALMAGALINNPWTIVPILGATYWTGAAILGHSDSPSFDWHDLGFYA
                     IYEQVMPYVIPFALGGTLLSLIAAAVSYPLAFYFINKHRRQATPSHAMPLPPHDPLG"
     gene            13052..14479
                     /locus_tag="VLD60_03260"
     CDS             13052..14479
                     /locus_tag="VLD60_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSB44000.1"
                     /translation="MAQQETSRLSHAPYDGSALIADPIHKYVSFTVPYSQAAPQERTE
                     KDLIDSPWIQRLRYIYQLQSARWVYPSAEHTRFVHSIGTMHVAGRFARHLYPFLKKVS
                     GDIPSASYVEEFLRVTALVHDIGHGPFCHFFDDNYLQAFQASHEKLGQIIIRDHLGPI
                     IKKIRRSPSGPFAKGEELSPDQIAHVILKEKGKDNSRLPRWLNMLQPVISGSYTADNL
                     DYVLRDSYMCGVAVGPVDLTRLIHYSIVTQKGFTIHKTGLPALQMFLNTRMYLYSNVY
                     FHRTTRAIDIHLRDIFGDTMKLVFPHDPRKKMDEYLTLTDWSLLEQVRSWKKSRHAAE
                     RRLGEEWARILGRDVKWKMAYSTVLKEKGQERGMDFPSHEHFEKQIQKELPSDLRHIQ
                     FRVDMAPLDPRPDPKDQRGNPLFVYDPSTRGVSTEPLEEFLDLLPTRLVQFRIYSLDH
                     RHDASLSRAAATVLNKTPSSIESNY"
     gene            complement(14574..15005)
                     /locus_tag="VLD60_03265"
     CDS             complement(14574..15005)
                     /locus_tag="VLD60_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44001.1"
                     /translation="MAQLQQRSKKAAPLLSHDELCKLVKGQWKRSVSADTDRVNVHLL
                     EALCFEKSDMDTAISGYRFVIDHAKEERHLPFIVPAYLRLIATLLRWGCDEEAGRAIE
                     QFEHRFSASQDDAFAPSAGQRKVLEELKSLLWERQSLKVSA"
     gene            complement(15587..16597)
                     /locus_tag="VLD60_03270"
     CDS             complement(15587..16597)
                     /locus_tag="VLD60_03270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSB44002.1"
                     /translation="MIIPVFKQVRVRISRLDRTLITAVVLLTSVFPVELFPNTPPPPK
                     GIHGQYSGRQGQVLVVKVPESEEATEVKGAFLNRTVPFFREFRSGESPGYVALLGIDM
                     QDDPGTYELAVEIKHGERMKHLSYNVLVAKEKFSVEHLKLPKDKVDLDEKSLARWKAE
                     QEQVKQALAENSRLKLWHSSFVEPVNGRRSGIFGSVRIMNGQPRNPHNGEDISAPQGT
                     EVLATNDGVVRLTVDHFFSGRGIFVDHGLGFYSMYFHLSEILVKDGDLIKAGQIIGKV
                     GATGRATGSHLHWGVKLNGARVNPYALLDLPFKNGSSPGSTVFIPAPESTPAPEDLPA
                     RD"
     gene            complement(16594..17970)
                     /gene="pafA"
                     /locus_tag="VLD60_03275"
     CDS             complement(16594..17970)
                     /gene="pafA"
                     /locus_tag="VLD60_03275"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HSB44003.1"
                     /translation="MKQRIFGLENEYGLIFSPNGRIYLPMEKVLGYIFEGLIPNSWPS
                     NAFLVNGARFYQDTGCHPEYSTPECDNILDLVVHDKAGERLLEACLPAAEERLREEGL
                     SGEIYIFKNNTDSLGNTYGCHENFLMRRDVDFWKVTEQLIPFFVTRQVYSGAGKVLKV
                     SGKPQFFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAERYRRLHIIVGDSNMSEL
                     VTYLKVGTATLVLSMVEEGYAVHGMELEDPVKAIREISRDPTVKRKVKLDDGRQMTAV
                     EIQRVYLDRAQEYLAQQDHDPILDDVWKRWASVLDKLEEDPMQLVREVDWVTKRHLIQ
                     SYIDRKGCGWDDPRVFLLDLQYHDVKRTRGLYYLMESRGLIERVVEEDAVQRAMSTPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRVDELVSQ
                     VSGLRFYR"
     gene            complement(18063..18746)
                     /gene="prcA"
                     /locus_tag="VLD60_03280"
     CDS             complement(18063..18746)
                     /gene="prcA"
                     /locus_tag="VLD60_03280"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSB44004.1"
                     /translation="MPMPYYVSPEQMMQDKAEYAKKGIAKGRSIIAMEYMNGILLAAE
                     NPSATLHKISEIYDNIAFAGAGKYSEFENLRKAGIRHADLKGFMYSREDVTARSLANG
                     YSQSLGTIFSQEMKPLEVEILVVQIGHNGHTNEMYRISFDGSIIDEKQYAVIGGKSEA
                     VQTVLKEKVPAQLPDLKTALTLCIGALEQTGNQKLSLESLEVAVLDRTRDNRKFRRYT
                     PAETRQLLS"
     gene            complement(18749..19576)
                     /gene="prcB"
                     /locus_tag="VLD60_03285"
     CDS             complement(18749..19576)
                     /gene="prcB"
                     /locus_tag="VLD60_03285"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSB44005.1"
                     /translation="MKLPFLPNHDDASFFDFLTAQHPELTPGGRRWAENQPSRLESSR
                     TGGGVAVPQATTVLAIKYQQGVIIAGDRRATEGYQIAERRIEKVFKIDDYSAMAIAGA
                     AGPCIEMAKLFQTELEHYEKLEGMQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYVG
                     FDLKRSEGRIFKYDLAGGRYEESDYHAIGSGGKDARNTMREHFSRGLAEPDALKLALL
                     ALYNAADDDVGTGGPDLIRGIYPTAKIVNAAGITDVSEQQILGVYNSLIETRRSKER"
     gene            complement(19573..21075)
                     /gene="dop"
                     /locus_tag="VLD60_03290"
     CDS             complement(19573..21075)
                     /gene="dop"
                     /locus_tag="VLD60_03290"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HSB44006.1"
                     /translation="MRESTPLTTPRVMGTETEFGIASRDASATDPISNSLAVIGHYPG
                     LSVPHAVWDYENENPLLDARGFEVDGERERPNPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECTNPREIVAYERISERILTQCLDEMTRVRGSEQFVLYKNNSDGKGNSYGYHENY
                     MLSRSVPFERIVKALAPFFVTRPIYAGAGKVGAENQTSSADYQISQRADFFECLVDLN
                     TMVKRPIVNSRDEPHADYGKYRRLHVIVGDANMAELSTYLKVGTLAIVLDLVEAGVEL
                     PQVELDDPVLAIKQVSRDLTMSGTLKLTGGRSTTALEIQRAYLKTAMDFYACHELNQV
                     TKDVLVRWEDVLDKLERDPRLLARELDWVAKRQMMESYMDRKGCGWNDSRVRLMDLQY
                     HDVRPDKGLYYTLERSHLIERVVQDVEVSRAEFMPPPGTRAYFRGRCIGKFSKSIYGA
                     SWTSVLFDVGNTTIKKVPLMDPLRGTESLTADLLDQSDTAEALLARLKAS"
     gene            complement(21087..22856)
                     /gene="arc"
                     /locus_tag="VLD60_03295"
     CDS             complement(21087..22856)
                     /gene="arc"
                     /locus_tag="VLD60_03295"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HSB44007.1"
                     /translation="MADPKGSPSRLRSLRDSVKHITKRLSEGEADVIHRNDEQHREIE
                     KLRVQIQSMEEEIRRLYQSRYQLDQATKQNEKLAMTLQEAKAQIEALRAEVEKLTAPP
                     STYAIFSSLNQDGTGNVYVSGRKMKVSLHPSIKANGLRKGQEVILNEALNVIEARGFD
                     VQGEVVRVKDVLEGNRALVTLHFDEEKVAELGDPLLAERLSVGDHLLYDPRSGCVIEK
                     LPKSEAEELVLEEVPDVDYAHIGGLHRELEQVRDAVELPFLHPELFAEFHLSAPKGVL
                     LYGPPGCGKTLIAKAVANSIAKKLGHLTGKEVRSYFLHVKGPELLNKYVGESERQVRE
                     VFKKAKERAADGTPVIVFFDEMDALFRTRGTGISSDIESTIVPQFLSEIDGVERLRNV
                     IVIGASNRQDLIDPAVLRAGRLDVKVKVGRPDAVAARDIFSKYVGANLPFDHEELQRH
                     GGDVKALVEHLTDMTVDAMYATSEENKFIEVTYANGEKEVLYFKDFASGALIEGIVSR
                     AKKYAVKRAIAQEGRGLRSEDLIRAIREEFKEHEDLPNTTNPDDWAKVAGKKGEKIVH
                     IRTISGGPAESRQIETITTGHYL"
     gene            complement(22990..23622)
                     /gene="thiE"
                     /locus_tag="VLD60_03300"
     CDS             complement(22990..23622)
                     /gene="thiE"
                     /locus_tag="VLD60_03300"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSB44008.1"
                     /translation="MRKLSSRLLVVTDRHQTNGRPLEAILEKVLAAGSPVIQLRERDL
                     APRALLALARTVQTLATQKGSQLVINDRIDLALVLDGVGVHLRSNSLPVSVARRLLGE
                     QRLLGVSAHSLDEAVQAEAQGADYVVLGPVYETPSKQAFGPPLGLEKVKEVCRCVRIP
                     VIGIGGVTPARARDVRRAGAFGVAVITAILGATDVEAAAREMLDAVSADP"
     gene            complement(23721..24494)
                     /locus_tag="VLD60_03305"
     CDS             complement(23721..24494)
                     /locus_tag="VLD60_03305"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HSB44009.1"
                     /translation="MHDDRLVIAGREFKSRLWVGTGKYKDFVETKKAIEVSGADVVTV
                     AVRRVNITDRSKENLLDYLDPKKYIILPNTAGCYTVEDAVRYARLARAAGVSDLVKLE
                     VLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAPI
                     GSGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGARDP
                     IAMAEAMKYAVHAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            complement(24484..24744)
                     /gene="thiS"
                     /locus_tag="VLD60_03310"
     CDS             complement(24484..24744)
                     /gene="thiS"
                     /locus_tag="VLD60_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSB44010.1"
                     /translation="MTDGMQIQVNGERRDCRAGLTVGDLLRELAIKTERVAVELNLEI
                     LDRKEFDHRGLKEGDRVEILSFIGGGAPLGRNGEREACSHAR"
     gene            24854..25348
                     /locus_tag="VLD60_03315"
     CDS             24854..25348
                     /locus_tag="VLD60_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HSB44011.1"
                     /translation="MATPLNNQQIATLFRSMAELLAAQRANPYRVRAYRRAAETLLAQ
                     EEDVAEIAKRNALEEIDGIGKDLSEKIVEFLRTGKIQAFEALKTPLPDQVRDWARLPG
                     LSESLIAYLYSRLNIHTLDELEQLVRSHMLRTLPGFSGSEEKLLQAITILRGHSSLKK
                     DGVP"
     gene            complement(25326..26654)
                     /locus_tag="VLD60_03320"
     CDS             complement(25326..26654)
                     /locus_tag="VLD60_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSB44012.1"
                     /translation="MVVIALVSGVLIGKGWERTGHATESYEELKTFSEVLSQVQRNYV
                     DETKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFGGVGIQIGVKENRLA
                     VIAPIDGTPAQRAGIKAGDYITKVNDESTKDLSLMDAVQKMRGPKGTKVNLTIQRDGS
                     PDPLQFTLVRDTIKIESVKSKVLDNIGYVKLTQFQEATGRDLGKVLKQFKEQKLQATI
                     LDLRNNPGGLLTAAVEVSEQFLPSGKLVVYTKGRESKKDEWFAKGKDQMDDSPMIILV
                     NEGSASASEIVAGALQDYGRAVIVGTTSFGKGSVQTILPLGDGSGLRLTTAKYYTPKG
                     RSIQSTGITPDIVVKPQAPTTVAKADVKPGEKDADAKSTKPPVTAAPGKEQSSVNGKG
                     HEEGTPKNGTTPAVPSADASGEPALEQDVQLQKAVEMLKTWKIFKELRPS"
     gene            complement(26727..27914)
                     /locus_tag="VLD60_03325"
     CDS             complement(26727..27914)
                     /locus_tag="VLD60_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSB44013.1"
                     /translation="MSKSTGRWMSMLVWSLLAWSMPAGAWADSINEKIDKERKALEQL
                     KDQIEEKRRQADEASKKRESILQGIQTLDERHMRYRQAHQEINRKLRKKDQEIETINA
                     QLSRLQDSVQERRDAISARLRVQYIEGRFGHWKTLLASDSYGDFQRRLRYLSAVSERD
                     YDLLETFKGDMARMQEAERQREEARVGMLAYKRSTEKQLEEIKAVKKEKRVYLTKLTH
                     EKESYERALQELERSASRIDNLLRELENRRRAAAARPPSGALAPRGVKGGLPWPADGS
                     VITYFGRQKHPTFNTYVQRKGIEIKTAEGSSIRAVMGGNVVYADWLKGYGLVIILDHA
                     NGFFSLYAHASRILTSVGAQVGSGQAIGETGDTGMTGENTLYFELREGAEPVDPLQWL
                     ARR"
     gene            complement(27911..28810)
                     /gene="ftsX"
                     /locus_tag="VLD60_03330"
     CDS             complement(27911..28810)
                     /gene="ftsX"
                     /locus_tag="VLD60_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSB44014.1"
                     /translation="MRSIFYLLREAWANIWTNRTTTMVAILTTAFTLACVGIFLLLYV
                     NLRAAAGWLQDDIKIMVYLDDRAAATTITEIEQQLRGDRAVAAQHFISKEQALGEFKA
                     QFPSESHLLEGLGQNPLPASFVVTLGPAFRSPDAVKRWAERVGQLAGVVKVDYNQDWI
                     DALSTVIRSIEVVAIGIGLVLSAAAVTIIANTIRLTLFARRDEIAILRLIGATKSFIR
                     IPYLLEGAVLGGLGSAVSLGMLKLLFELFRQQMRMTGRFSGMEQLMMFFPLSVCLMLV
                     AIGVGLGCAGSFVSLRRFDEAKV"
     gene            complement(28807..29475)
                     /gene="ftsE"
                     /locus_tag="VLD60_03335"
     CDS             complement(28807..29475)
                     /gene="ftsE"
                     /locus_tag="VLD60_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSB44015.1"
                     /translation="MIQLIHVTKQYERRPALNDVSLEIEKGEFVLLMGPSGAGKSTLL
                     RMLIGAERPDEGQVFVNGKNVTTLKASEIPFLRRKVGTVFQDFRLLPKKPVFENVALP
                     LLVQGVSPGDIKRKVTEALRAVGVEHKRDQLPVSLSTGEQQRVCIARAIVNGPVVLLA
                     DEPTGNLDPALTGEIIELFKLINARGTTVVVATHDPSVMAQVKRRVVTLVQGSLMPEQ
                     GVGV"
     gene            complement(29483..29875)
                     /locus_tag="VLD60_03340"
     CDS             complement(29483..29875)
                     /locus_tag="VLD60_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSB44016.1"
                     /translation="MTLLDPRRLFGQEGEATAERYLRRKGYRIVAKNLRSTLGELDLV
                     AEDGKVLVFVEVKARRSEDFGGAIHAVHRHKQEKLIQLASQYLAKHHLTDRACRFDVV
                     LLQGTDASAPQIEHIQNAFDVSRSEPRW"
     gene            complement(29872..30573)
                     /locus_tag="VLD60_03345"
     CDS             complement(29872..30573)
                     /locus_tag="VLD60_03345"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSB44017.1"
                     /translation="MRAGTHTVGNFVGPTDEFELEARRCGYRRIAGLDEAGRGPLAGP
                     VVAAAVVLPSRCHLIGCDDSKQLSESDRERLYQIISERAVGIGIGSASQEEVDRLNIL
                     EATRLAMRRAIAALSPQPDCLLIDAVALPGCAIPTRSIIKGDALSISVAAASIVAKVT
                     RDRLMAEYHQLYPHYNFLSHKGYGTDEHLRQLAVHGPCPIHRKTFAPVAEMIAHLSSQ
                     QQTVSRQEESQRTIA"
     gene            complement(30604..31035)
                     /gene="rplS"
                     /locus_tag="VLD60_03350"
     CDS             complement(30604..31035)
                     /gene="rplS"
                     /locus_tag="VLD60_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSB44018.1"
                     /translation="MNQLERVQRSLQKKSVPHFEIGDTVRVHVKVIEGEKERIQVYEG
                     AVIARKGSLNTEMFTVRKVSYGVGVERIFPVHSPIVTKIDVVRQGKVRRAKLYYLRGK
                     KGKFSRVEDREFVGEGKAQPGSVRATESADAPEEAAAAAKS"
     gene            complement(31040..31822)
                     /gene="trmD"
                     /locus_tag="VLD60_03355"
     CDS             complement(31040..31822)
                     /gene="trmD"
                     /locus_tag="VLD60_03355"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSB44019.1"
                     /translation="MRCDVITLFPEMVAPVLGSSILKRAQEKGLLDVKVHNLRDYTLD
                     KHKVADDVPYGGGAGMVMKAEPILCAVEQIKAGYEQAGEQIRLLLPSPHGRPFTQVYA
                     QDLAAESRRLVILCGHYEGIDERVRVALQPEEVSVGDYVLTGGELPALILIDAAARLI
                     PGVLGDPESIVEESFTDSLLEYPHYTRPADVRGMTVPEVLLSGHHEAIRLWRRKEALR
                     ATYEKRPDLLKDRVFTVEDRQLLDDIIREGVRGTPVRCGEEG"
     gene            complement(31812..32348)
                     /gene="rimM"
                     /locus_tag="VLD60_03360"
     CDS             complement(31812..32348)
                     /gene="rimM"
                     /locus_tag="VLD60_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSB44020.1"
                     /translation="MTTVQADLVTIGKIERPFGVHGAVKVRSLSDVPGRFDSLGEVSV
                     MELTGRTVDRTVTHVRRAGPTYIVQFACITTPEEAGTLRGGLIQVPRCEPSARTGNTL
                     YECDLIGLTVVDEDGRELGLVETIWELPGHQVFVVRKNDRELLIPAAKNFVVKVDLVQ
                     RRMTVRGIEGLVEDRHAL"
     gene            complement(32352..32636)
                     /gene="rpsP"
                     /locus_tag="VLD60_03365"
     CDS             complement(32352..32636)
                     /gene="rpsP"
                     /locus_tag="VLD60_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSB44021.1"
                     /translation="MAVHLRLTRTGRHKRPVYRVIAADSRKPRDGRFLEILGIFDPLK
                     EAGLPELKQERVLSWLHQGAQPTVTVKTLLRRSGLWKQFEAEKAAKKKKA"
     gene            complement(32690..34036)
                     /gene="ffh"
                     /locus_tag="VLD60_03370"
     CDS             complement(32690..34036)
                     /gene="ffh"
                     /locus_tag="VLD60_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSB44022.1"
                     /translation="MLDSLSEKFEKILKKLRGSGVLTEQNIAEALKEVRLALLEADVN
                     FKIVKDFIERVREKAVGQDVLQSLTPGHQVVKVVWDELRAMMGGERAGLSLAATPPTV
                     VMMVGLQGAGKTTTCGKLAKLFKNQGKRVLLVAADPRRPAAGDQLASLGRDLGIDVHR
                     LDQAGASQTDVVRICQAGVERGRDQGFDLVVLDTGGRLHIDDELMGELVAVKSAVQPH
                     EVLLVADAMTGQDAVTMASQFDQRVGLTGIILTKVEGDARGGAVLSIRAVTGKPIKFL
                     GIGEKLDALELFHPDRMASRILGMGDVLSLIEKAQETFTREQAEEAQKRLTSSTFTLE
                     DFRAQLGQVSKLGSLDQILGMLPGGQKLKGAMDGHMPEREMKRVLAIIDSMTARERRD
                     HTMVNGSRKKRIARGSGTNVMEVNRLIKQFLSARKIAKVMTGAGGRRQLAQLLRSM"
     gene            34194..34511
                     /locus_tag="VLD60_03375"
     CDS             34194..34511
                     /locus_tag="VLD60_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HSB44023.1"
                     /translation="MVVSVIRRGWSTSYEAVGVRKSPWVLLLFLLIGGLLGGILGEVL
                     QVMAPKGGTIQAFFITAVTPGISPPLTIDLILLKITLGFSLKISLLSVLGMFLGIYLY
                     KNV"
     gene            complement(34508..36511)
                     /gene="uvrB"
                     /locus_tag="VLD60_03380"
     CDS             complement(34508..36511)
                     /gene="uvrB"
                     /locus_tag="VLD60_03380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSB44024.1"
                     /translation="MPQFKLEALFKPCGDQGQAIDKLVAGIEAGLKHQALLGVTGSGK
                     TFTMANVIERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QTDTYIAKDSSINDAIDQMRHAATTSLLQRNDVVIVSSVSCIYGLGSPEVYHGMVVYL
                     EEGMEIRREKILAKLVEIQYARNDVDFHRGTFRARGDVIEIFPASNEAISVRIELFGD
                     VVDAIHEIDPLTGKSLKKLPKVAVYPNTHYLIAPDRYEKAITGIEEELDERVAYFKKH
                     NQLVEAQRIAQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGEPPPTLIDYFPKDFLL
                     IVDESHATIPQVGGMYEGDYSRKRTLVEYGFRLPSAVDNRPLKFPEFERCINQVVYVS
                     ATPGPYELEHVNGEVIEQIIRPTGLLDPLIDVQPAKGQVDHLLGQVRAEVSAGGRVLV
                     TTLTKRMAEDLSEYYHELGIKVRYLHSDIKTLERAEIVRDLRRGVFDVLVGINLLREG
                     LDIPEVSLVAILDADKEGYLRSFRSLIQTAGRAARNVRGRVTLYGDVVTDSMKAAIDE
                     TVRRRRIQADYNAAHGIVPASIKKEIPTLEYADAYVEPEQLALAAESQHEYGTEEACE
                     QLIKRLELEMKAAAKELDFERAAAIRNRIRTLKLKDLELKSEV"
     gene            complement(36643..>36885)
                     /locus_tag="VLD60_03385"
     CDS             complement(36643..>36885)
                     /locus_tag="VLD60_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44025.1"
                     /translation="EEREYLRMVRQLRIQTKKLPLPAFATMPPVAVAPADDQQARRPR
                     PPRHGDSTNRRFHENGSGESFRRHGASSGGPRRFGR"
BASE COUNT         7846 a        11399 c         9576 g         8064 t
ORIGIN      
        1 ataggtcagc ggggtagttt tgtacaacag gaccatggga gttcccacca ccgcggcctg
       61 caaggtggcc gtcccggacg cgatcagcaa gacatccgcg gcagccatca cttcgctggc
      121 ctgatcccgg acgacgcgaa ccgggacgga actctgatgg agcaatcctt ggagcagatt
      181 atcatcaatc gaagaggcct gcgccaggat aaaccgcgtt ttcggctcga cggctgccag
      241 tttcgccgcg gtctccaata gcaccgggag cagcatggtg acttcggcga cccggctgcc
      301 gggcaaaagg ccgacgaccg gaccagagtc ttccagatca aaggtccggc gaagcctttg
      361 tcgatcatac tgaggcgcca cggcatcaag gagcggatgg ccgacgaagg tgcagcgtac
      421 acctgctcgt cgatagatct ccggttcaaa cggcagaatg accaccacat gatccacgcg
      481 gcgctgaatc cacttgatcc ggcggggccg ccaggcccaa atctgcggag caatgtaata
      541 gaggacccgt cggccggcag cttttgcaac gcgtgcaaaa tgaaagttca gcccggggtt
      601 gtcgatcagg acgacgagat cccaggcctc gctcttgagc actcgtcgga ttgcgcggat
      661 ccgctggacc atcgcacgaa tagcagacag accgatcaac cccatcacgt ccaagtgagg
      721 gactccggga accaggatga cgcccgccgc ccgcatcgcc gctccgccga tcccgaccaa
      781 ttcagccttc ggatcaatag cccagatcgc ccttgccaaa ttggcgccgt gcagatcgcc
      841 ggaggcttca cccgtcacaa ttaaaattcg catagattca gaagacgggg tatggggggg
      901 taaagggata agggggtaaa aatctgtgtc ctctctcccg ttacccctat ataccctaac
      961 tacccctttg tcccttcgtc ctgccgcgcc ataaacgccg tgatcgcctg aagtacctgc
     1021 tgtgcgacgt cgagcgccgc ggcgccgtct tctcctgata cgaccggtcg cgtcccggtg
     1081 ccggccgcat gcagaaacga ttcgagttga agcttcaagg gttcctcttc accgccttgc
     1141 agctgctcga cgtcgacggc ggggcgttga cccgctttgg catcgcgccg gcagatcacg
     1201 ccttgtctcg attgaaagtc gatcgagatg tatttgtcct gttggaatac gcgcaaccgc
     1261 cgcattttgt tcattgacac gcggctggcc gtcaaattgg cgacgcatcc gcccttgaat
     1321 tgaatgcggg cgttggcgat atcgatcgtg gatgacagga ccggcacgcc cgccgcttga
     1381 atatcttcca ccggaccggg gttgaatgac agcaccaggt cgagatcgtg gatcatgaga
     1441 tcgagcacga catccacatc ggtacctcgc tcgctgaaac tgctgagacg gtggcattcg
     1501 ataaacacag gccgtccgat gtggggccgc atcacctgca tcaccgggtt aaaccgctca
     1561 ctatgcccga cctgcaaaca gcgaccttgc tgccgcgcga gttgaaccaa ttcgtggcct
     1621 tgcgcggagg ttacggcgat cggtttttcg acgagcacgt gcttgccggc caaaagacag
     1681 gccttcgcga cagcgtaatg cgcggacgta gggaccgcaa cgctgaccac ctcgacaagc
     1741 ggaaggagtt cgccgaggct gcgggtcgca cgaacccggt gccggtcggc gatcagttgg
     1801 gcccgatcca ttgactgatc agtgaccgca acaagctgcg ctccaggcag agaggcatac
     1861 aggcgggcat ggtgctgccc gagatgccct acaccgatga cgccggcttt caaggctgtc
     1921 acggtgcggt agtccctccc gtcgcgtcag gtttgacaag cccgacgacc gcaatgccgg
     1981 cggagcaggc cttttcaagc atgagttcgc gatccaacaa aatcgtacga cccgcctcca
     2041 cggcaagtac cgaagccttt accgatttca tcacatcaat cgtcctgggg ccgacggcgg
     2101 gcagatcgaa ccgcaaatct tgctgaggct tgcaccgctt cacgaccacg gcaccttcct
     2161 tcgcaagttg accgccacgc ttgatggcct catccgtccc ttcaacggct tcaaccgcca
     2221 ccacgacacg atccttgatg acgacgcatt ggccgatgtc gagccgccca atgtcgtgcg
     2281 ccacttccca cccgtagcgg atatcctccc attccttctg actcggtaca cgagccgtca
     2341 acgtcccctc ctccgcaaga ataccttcaa ggccgaaggt cgattcgcaa atactgatcc
     2401 cttcccgctc gagttccttg gcaaactccc ggagaatgtc gtcgtctttc cagagagcaa
     2461 ggcgcgtggc cagcgccagc gtgcgaaagt ccgggcgcac ggtcgtaaac acatgcgtct
     2521 tcttgatgcc gcccaacata acggcctggt gcacctcatc ctccttgaag gccttgatca
     2581 gcttgttgag ctggccgatc ttgatccaat ggatacgatc gacgtggttc gccaagtcgg
     2641 gatccgtctc gccttcgtgc gcgacggccg agaccagata cccgagtttc cgcgcgttct
     2701 cagcaaaaat aatcgggaaa cggccgtttc cggcaatcag gccgagtcgt tcaccgttgc
     2761 ggacctgggg ggctagcgtc acaggctcta ctcctcgtct tcctgctcct tggccaccga
     2821 ccgacaaata ccacgcttcg tcccctcaag aaacgccacc acggccatca catccggttg
     2881 atcgccgaat tccgccttgg ccagcctcgc ggcttcggct gttcgatgtc ccgatcgaaa
     2941 gagaatgtca taggcgcgtt tcaacaccgc gacccgatcg cttgagaatc catgacggcg
     3001 cagcccgacg gaattcaagc catacatccg ggccctgtaa ccgcctgccg ccctcatgaa
     3061 cggcggaaga tcctggccca acgcacaaca cccaccgacc atcgaatagg cacctacgcg
     3121 cacgtactga tggattccgg tcagcccccc gatgatggcg tgatccccga tcgtgatatg
     3181 cccggctaaa ctggccgcgt tcgccatgat cagatggctg cccagacggc agtcgtgtgc
     3241 cacgtgcaca taggccatca gaaaattctt gttgccgatt gacgtgatcc ctccgccctg
     3301 cacagtcgcc cgattgacgg tcacgtactc gcgaaaaata ttgtcgtcgc cgatgatcac
     3361 cttggtcggc tctcctttat atcccaaatg ctgaggcggt cccccgatcg agacaaaggg
     3421 gtggagttcg caccgttcgc cgatttgcgt ccatccttcg accgttacgt gtgaaagcag
     3481 tcgtgtccct tttccaatcg tcacgtgctc tcccacgacg cagaatggac cgaccaccac
     3541 gtcatcggcc agctgcgccg cagaatgaac gatggcatga ggatgaatct gcacgctgaa
     3601 cctccggaga taatgtcaat ggtgacaggc tgctcgtcac acgcatgcac agcagggacg
     3661 gttcgctggg ctcggcgtgg cggccgtcaa ttgacggtta acgatcgtcg cgcttaagcc
     3721 ttctcttcgg tcaccatcgc ggtgacttcc gcttcacaca cgagctcatc ttcgacaaat
     3781 gccttcccct gcattttcca gaagggcgcc cgcttcttca cgacatcgac ctcgaaacgc
     3841 aggcgatcgc cgggcacgac cggcttgcga aacttcgccc catcgacacc ggtcaagtac
     3901 acgaccggcc tcttcgcctc ccccatagac ttgaacgcca gcagccctcc gacctgagcc
     3961 atcgcttcaa gaatcagaac gcccggcatc accggtctcc ctgggaaatg cccctggaaa
     4021 aacggctcgt tgaccgtgac gttcttgatc cccacaatcc gccggtcggg atccaattcc
     4081 aagacccgat cgaccagcag aaagggatac cgatggggca agagcgcctg aatatcggct
     4141 tgttccatga ctgacatcag caaaacctcc tcattggtct atggggcggg acagtagcac
     4201 ggctacttgt tctgacgatc gaactccttc accacctgct cggtcatatc caagcccggc
     4261 tgatgataga tgacgatctt gatgaccgca tcgttcccct tatcaataat ggcaatcgcc
     4321 ccttctcgtt gcgcgacgac ttgggccgcg tcccgaactt ttttcgaata ttcagcgacc
     4381 gtttcgcgct gtttctgttg gatctcacga ttaaagtctg ccaagcggcg ctggtaggct
     4441 tccactttgg cctggaactg cgcttgcttc tcctgtttgg ccgtctcgct caacttgctg
     4501 tcctgagcgg cctgctccaa ttccttcaat tcctggtcgt cggcattaat gatcttctgt
     4561 ctggttgccg cgtacgcttt caactcttcc aatgcccgtt tcccggcttt cgacttctca
     4621 atcacaagtt gctggtccat caccgcaact ttcgataggt cggctgcaaa cgcggccgat
     4681 aacatcgcac tccagatcgt cacgaccgta atcaggttca cgagtttgct tgctctcatc
     4741 tgttccctcc tccggatagc gtccgctcag atctcactct cacggatact cgcgattgaa
     4801 ctcttcgatc acttggttcg taatgtcgag ggattcatca ccaaaaatag cgggaccgcc
     4861 tttgcccttg tcaatcacca cttgcaagcc gtggcgcttc gccactttgg cgacgacgac
     4921 ttccacctta tcacgaaacc cttcgtaggc ttccttctgt ttctcttgga tctctcgatt
     4981 caactctccg gctttctgct gatactccgc catgcggcgt ctgaatgctt gttcccgttc
     5041 actctttgcg gcgggactca ggacgctcgc ctgcttgatg aaatcctcct ccatgcggcg
     5101 cagctctttc tcctcgatct cgagcaaggc ctgacggttc ttggagaagg cggccagcga
     5161 gtccttggtt ttcttgccag cattggcctc attgaggaga cgcgccggat cgacaaggcc
     5221 gatctttccc tcaacctttg cgcccgcacc ggcacatccc atggcactgc cgacccagaa
     5281 agccaacccg atcgacaacc agaagatctt gtttaccgtt tgagactgga tcacgaaccc
     5341 acccctcatc cagcaaccgc taaaacagcg acccgatcgt gaactcgaac acgcccttgc
     5401 gctcgccctc gcgcgcatca agattgatcc cgtaggccgc ccgcaatgga ccgaaaggcg
     5461 aaatccatcg gccttcaagg ccggcagcct ttcgaagatc gaacgagaca gattcatcct
     5521 gagcaaaccc tttcccatag tcgaagaaaa tcaccccgtt caacttcgct tcggacgaga
     5581 tcgtgaagat aaagtcattg ttgaagatca gctgcttttg cgcgccgagg agtgtttttg
     5641 tgttcgggtc gaccggtcct gcacggccga attggaaccc gcgcatcgta ttgatgccgc
     5701 cgacgaagaa gagctcggtg aggggaaccg gttccccgtt gatcgagttt gcctctccat
     5761 agcgggcatg gatggagaag cgcgtgtcgt acggaagcgg cgtgtatttg agcacatcaa
     5821 aattatattt atagaagtta ttggaaccgc cgaggtaggg agtccccagg tcaaaccccg
     5881 cactcgttcg ccaccccgtt cgaggatcaa ggtaatagtc ccgcgtgtcg cgtgcgattg
     5941 atgttctgaa tccggtcgtg gtttgatttc ccagctgctg gcagatgatc aggcctggat
     6001 taaaaccgca gttgttattt ggatcgctgt agtccaactg ctcagccatt aaactgaagc
     6061 tgccggagac atattcggac agccagcgtc caaaggtaat actcgctcca gtcttctttt
     6121 caaaataggt gatgtagtcc gtcatgctgc ggtatatatc aagctgcatg gaggtcaatg
     6181 agtcattcag atacggatta cggaacgtaa tcaatccgag cgtccggttt tgcccgagtt
     6241 ggcctcgaat gcgccccatc catccgttgc ctccaaggtt tccctccgtg atatcggcaa
     6301 tggcgacgag cttatccaat gtgctgaacc cgccgccgat gctgaactgg cctgtcggtt
     6361 tctccttcac ccgcacattg agatcgacct tgtcgacatc gacttgctga ggcaggattt
     6421 cgacggtttc gaagaaattc aagttattca ggcgctgaaa gctgcgctta agtgcggggg
     6481 tgtcgatgac gtcctgctcg tctacgcgaa cctcgcggcg gatgacgttg tccttagtct
     6541 tgtcgttccc attgatgttg atttgccgga tccgcatcat ctccccttct ttgatgctca
     6601 ggatgatggt tgcggtgcgt tcttccgggt tgggagtgac gctgggcgtt acgtcggcga
     6661 acgcataccc cttactcccg tacagatcgg tcaaccgggt gatctcatcg cggattttct
     6721 ggcgctggaa gatttccccc ggcttgattt tcaacttctc ccgcagctcc ggatcttcga
     6781 acacggtatt gccccgaaac cccacttcgg ccacggtgaa tggctcccct tccacgacgg
     6841 cgtaggtcac gacgaaccat ttcctgtcgc tgctgagttc gacggaaggc aaaccaactt
     6901 ggacgttcaa gaagcccttg ttcaatagca cttccttgat ccgctccacg tcattattcg
     6961 actcctcgcg cttgaggacg ccggcatccg ataaaaatga cggcaactgc aacttggtga
     7021 agattccgta ccagggaacc cattccctgg tcgccatgac tttgaacatt tcttctttgc
     7081 tcgcggcgtg catgccgtcg aagttcacga ccttgacacg cgccttgtcc ccctctttga
     7141 ccaaaaacgt caatcgctta cgatcttcgt ccagcgtctg cacgacggga atgacttggc
     7201 agttgaagta cccgtcctcc tgataggtaa ggcggatttt ttcggcgctc tctttagcct
     7261 gctgctggtc caaaaaagct tggctcttga tcgtgatctt ctccttgagc ttgtcatcgc
     7321 tcatctcggt atttccatcg aatacaatct ccgtgatgaa cggcttctca cggacgagaa
     7381 acgtcaacgc catcccattc ggaatcggct ccgtctcgac ttggacatct tcaaaatatc
     7441 cggtgtcata gagaatcttg atctgccccc gaacgttttc cggcgtgtag ggatcacccg
     7501 gcttcagggt cagccgcccc tcgatcgcct gcagctcaat ttttttattg ccgcgaatct
     7561 cgatcgatcc gaccttcaca gcggttggtt gagcgatcgc ctcacaggcc agaacggcgc
     7621 cgtaaaacaa cgtgataagc aacaccaacg cccagcgcga cccctgtaag gacgacacct
     7681 ccctcaccag atcagcactc cacacatggc cagatagcat gacgaatcca tcgtcgagac
     7741 ggctcataag tgacgtggcg cctatcaccg ggagccccgt tgcgaacaac gccaaaccgg
     7801 agccgtcaag gacagcgacg atgaaggttg ttgacgtgag atgcatacgc gctcggcgca
     7861 gacacatcag ccgcggaccg gtgcgtgaaa aactctcgga ttatatgggg caagtatttc
     7921 aatgtaaagc ggttttggcc cgacctgatg cctgtcggga gcgaaccggg agatcgccgg
     7981 tttcttgaca tcccctaccc catcacatag tatctgcata tgtcgcgcac agaagttcgg
     8041 aaagccgtta tccctgccgc tggtctcggc acaagattcc tccccgcaac aaaagcctca
     8101 ccgaaagaga tgctgccgct ggtcgacaaa ccgttgatcc aatatgtcgt ggaagaagcg
     8161 gtggcatcgg gcatcgaaga catcatcatc gtcaccggtc ggggcaaacg ggccatcgaa
     8221 gatcacttcg accgatccgt cgaacttgaa gaaaatctga aggggaacgg aaaggctcag
     8281 gtgctcagcc agatcaggca catttcaaat cttgcgaact tttgctacgt acgccagtcg
     8341 gaagctctgg ggcttggcca cgcagtactc tgcgcccagc acctgatcgg agacgagccc
     8401 ttcgcgatca tgctaggtga cgaaatcatc gacgcgccgg ttcccggcct cgcacagttg
     8461 atccacgtct acaagaagcg ccgtggagcg gtcttgggca ttcaagaagt cccccaccaa
     8521 gaggttaacc gctacggcat catctcgccg gaccgaatcg cggacggctt atataaggtg
     8581 accaacctgg tggaaaagcc tgtaccagcc gatgcaccgt ccaacctggc cgtcattggc
     8641 cgctacgtgc tgcctcctga gatcttcccg attctcagaa aaactcggcc ggggaagaac
     8701 ggagaaatcc aattgacaga tgcgctcaag gagctggcaa aatcctctcc gatgtacgcg
     8761 ttggaagtga aggggaatcg atacgacgcg ggagataaat tgggattctt gattgccacc
     8821 gttgaatttg ggttgaagaa ccctactctt ggagcggatt tcgcagacta tctccggaac
     8881 cggccgaagt cctccggcga acaacgccgc cgcggcagag cctaaggcca cccctgtcat
     8941 caccgtacgc gaaccgcagt gaaatcgtga gacaatcccg atgagcgatt cgattgaaca
     9001 agacctgacg cctccccaaa acgttgagat ccccgaccag ctcccgctgc tcccggtgcg
     9061 ggatatcgtc gtatttccgt acatggtcct tcccctcttc gtcgggcggg agatgtcgat
     9121 caaagcaatc gaagccgcgc tggcgggcaa tcgtatgatc ttcctggcga cgcaaaaagc
     9181 cttggatgtc gagaatccga cccctgatga tatccatacg atcggcacgg tcggaatcat
     9241 tatgcggatg ctgaaactcc cggacgaacg gatcaaaatc ctcgtccagg gtctctcgaa
     9301 agccaaggtc tccgggtata tccaaaccga gccctactat tccgtgcgga tcgacaagat
     9361 tgaggaatcc aagcccgtga gctccgccct cgagagcgaa gccgtcatgc gaacggtgaa
     9421 ggagcagatc gaacggattg tgagcctcgg caaagtctta atccccgacg tcatggtcgt
     9481 catcgagaac ctggaagatc cgggccgcct cgccgacatg atcgcgtcga acctcggcct
     9541 caaggtggag gtgacccagg cagtcctcga aatcaccgat ccactcaaac ggctccgcca
     9601 gatcagcgaa atcctggcca aggaaatcga cgtcctgtcg atgcagcaga agattcaggc
     9661 ccaggccaaa ggggagatgg acaagacgca gcgggaatac tttctgcgcg aacagctgaa
     9721 agccattcag aaagaactgg gcgagttgga cgagcgagcc gaagaggtcg ccgagtttcg
     9781 caagcgggtc aaagattcca agatgccgga gaaggtcttg aaagaggccg agaaacaact
     9841 caaacggctc gaaaaaatgc atccggacac cgcggaatcc gccacagtcc gcacgtacct
     9901 tgaatggatg gtggaactcc cctggtcgaa gaaatcgaaa gacaacctgg acctgaaagc
     9961 cgctgccaag gtgctcaacg aagatcacta cgacctcgaa aaggtgaagg aacggatact
    10021 cgagtacctt gctgtgcgga agctcaaaga gaagatgaaa gggccgattc tctgtttcgt
    10081 cgggccacct ggtgtcggaa agacctcgct ggggaaatcc atcgcacgcg cgctcggccg
    10141 tgagtttgtc cgcatcagtc tcggcggagt ccgcgacgaa gccgaaattc gaggtcatcg
    10201 acgcacctac gtcggtgccc tccccggccg catgatccaa ggcatgaagc aggcgggcac
    10261 caacaacccg gtctttatgc tcgatgagat cgataaggtc ggcatggact tccgtggcga
    10321 tccatcagcg gcgttgctcg aagtgctcga cccggaacaa aacagtacgt tcaccgatca
    10381 ctatttgggc gtgcccttcg atctttcgga ggtgatgttc attaccaccg cgaatctaat
    10441 cgatcccatc ctgccggcct tgcgagaccg catggaggtc atcgagattc ccggctacac
    10501 tgaagaagaa aagctgggta tcgcccagcg ttatctgatt ccgcgtcaac tgtctgaaca
    10561 cggaatcacg gaaaaacatg tgcggatcgc cgagccggcg atccgacaga tcatcaccaa
    10621 ctacacgcgc gaagccggcg tcagaaacct cgaacgcgaa atcgcgaatg tcatgcgcaa
    10681 agtggcgaag aaggttgcgg agggcaaggg cctcggattc cccgtgaacc cggcgaacct
    10741 tcacaaatat ctgggcgtcc ccaagtacct cccggaatcg gagctcgaga aagacgagat
    10801 cggcgtcgcc acaggactgg cctggacgga agcaggcggc gacgtgctct acatcgaagc
    10861 cactgtgatg aaaggcaagg gccagctgac attgaccggg cacctcggcg acgtgatgaa
    10921 agaatccgct caggctgcct tgagctatgt gcgatcacgc gaaaaaacgc tcgggatcaa
    10981 cccggatatg ttcagcaagc aggacctcca tatccacgta ccggcgggcg ctattcccaa
    11041 agacgggcca tcggccggta ttaccatggc aactgcgatc gcctctgcca tggctcagat
    11101 tcccgctcgc cgcgacctgg caatgacggg ggagatcacc ctccgcgggc gcgtgcttcc
    11161 gatcggcgga ctcaaagaga agattctggc cgccaaacga gcccagctca cgaccgtgat
    11221 cctgccaaaa cgaaataaga aggatctgga cgagattccc aagcacattt tgaagggcat
    11281 ccaactcgtg ttcgcggaca cgatggacga tgtgatgcgg gtggcccttc gccgaacgcc
    11341 cagagccaca cgctcaaccg gcagaccgca agccccgcag cagacgcaaa agcgcgttgc
    11401 gaagaatcat aaatcaagac ggatagatcg aacggttcac gcacagtcag cgatcatggc
    11461 acattcgcac gaatagcctc attagcatgg catctccggc caggcccaag gccatctcca
    11521 cgattcggga atttgacctc atccgagctc tgcagcaaca ccatggcgcc ggtgactctt
    11581 cgattcttcg tggcatcggc gacgatgctg cagttattgc tccttctcaa aactgctggc
    11641 aactcatgac aacggacctt ttgacggagg gaatccattt cgatcttcgg actgccgttt
    11701 ggtccgacat cggttttcgc gcggcagcgg cgaacctcag cgacattgcc gcgatgggtg
    11761 gtaccccgca atacctattg gtcgccttgg cgattccccg gcacggaacc ggtcgacacg
    11821 tgcatcacct ctatagcggc atgatgaagg cttgccgccc ttacagtgtt cggctcatcg
    11881 gcggcgatat ctcggcgtcc gcccaaggtt ggttcatcag catcaccctg atcggatcag
    11941 tactgccacg ccgcgcactg ctccgaagcg gcgcgcgcgt gggcgacagc ctctatgtga
    12001 ccgggaccat cggagattca cgggcagggc tggaattgtt gcaacgatca cgtggcagga
    12061 aggccgctgc ttcgctttca gaaactcacc gacatttcct gattcagcgg catctccggc
    12121 cgactgcccg tgtcgaaatc ggccgatggc tctcgactca ccactgggcc acttccgcca
    12181 tcgatctgtc tgacgggctt tccggcgatc tccgccacat ctgcgcggaa agccgcgtcg
    12241 gagccgacat cgacttgtct gcgctcccga tctcacccgc ctgcacggcc tacgcggaat
    12301 ccacaggaca aggccccatc acgctcgcgc ttgccggcgg agaagactat gaactcctct
    12361 tcacagtatc cccacggcat cgcacgcgct ttgaacggat ggcagcagga cagcgagtcc
    12421 ggatgacgaa aatcgggacg atccggccag caagatccgg catccatggc ataccgttgg
    12481 acggtcaaag acagccattg ccggtcacca gctacgagca tttctgctcc tttaaatgaa
    12541 atgaagcatg aacggccatg gcctcttttc gatcaatact gagacaagtc cttcacctcc
    12601 aagaatcgcc ccagcgaaca gcggtcgctt tttctattgg agccgccatc gccttctgcc
    12661 cattttatgg cttacacatg atcttggtgg tcttctgcac ctgggcattc ggcctcaact
    12721 tcttggcttt gatggcggga gcgctgatta ataatccatg gacgattgtg ccgatccttg
    12781 gagcaaccta ttggacaggc gccgccatcc ttggtcattc cgatagcccg tcctttgatt
    12841 ggcatgacct cggcttttat gcgatttacg aacaagtcat gccttacgtc atcccatttg
    12901 cactgggagg aaccctgctg agtctgatcg ccgcagcggt ttcctacccg ctggccttct
    12961 atttcatcaa caagcatcgc cgccaagcaa ccccttctca tgccatgcca ttgccgcccc
    13021 atgacccgct gggctaagat gatggcaacc catggctcaa caggagacct ctcgcctatc
    13081 ccatgctccc tatgacggtt cggctctgat tgccgacccc atccacaagt acgtctcctt
    13141 taccgtacct tacagccagg cagccccgca ggagcgaacg gaaaaagacc tcatcgattc
    13201 cccgtggatt caacgcctcc gctatatcta tcaactccaa agcgcccgct gggtctatcc
    13261 ctccgcagaa cacacccgct ttgtgcattc catcggcacg atgcatgtgg ccgggcgttt
    13321 cgcgaggcac ctctatcctt tcctgaaaaa agtgtccggg gacattcctt cagccagcta
    13381 cgtcgaggaa ttcctccgtg tgacagccct ggttcacgat atcggccatg gtccgttctg
    13441 ccacttcttt gacgataact atctgcaggc gttccaggcg agtcacgaaa aactcggaca
    13501 aatcatcatt cgcgaccact tgggtccgat cattaagaag atccgtcgca gcccatcagg
    13561 cccctttgcc aagggagaag aattgagtcc cgaccaaatc gcccacgtga ttttgaagga
    13621 gaagggaaag gataactcac gccttccgcg ttggctcaac atgctccaac cggtcatatc
    13681 cggcagctat accgccgaca acctcgacta cgtgttacgc gattcctata tgtgcggagt
    13741 ggccgtcgga ccggtcgatc tgaccagatt gatccattat tccatcgtaa cccagaaggg
    13801 ctttacgatt cacaagaccg gcttgccggc actgcagatg ttcttgaaca cgcgcatgta
    13861 cctctattcc aacgtctact tccaccggac gacgagggcg atcgacatcc atctccgaga
    13921 cattttcggg gacacgatga aactggtctt tccccatgat ccacggaaga agatggacga
    13981 atatctcaca ttgaccgact ggtcattact cgagcaggtg cgtagctgga agaagtcccg
    14041 gcacgcggcg gaacggcgac tgggcgagga atgggccaga attctcggcc gggatgtgaa
    14101 atggaaaatg gcctatagca cggtgctcaa agaaaagggc caggagcgtg gaatggactt
    14161 ccccagccac gagcacttcg agaagcaaat tcagaaagag cttccgtctg acctcaggca
    14221 catccaattt cgcgtcgaca tggccccgct cgatccccga cccgacccca aagatcagcg
    14281 tggtaatcct ctattcgtct acgaccccag caccagagga gtgtcaacgg aaccgctcga
    14341 agagttcctt gacctacttc ccacccgcct cgtgcagttc cggatctact cgctcgacca
    14401 ccggcacgac gcatcgctgt cccgtgccgc cgcgacggtg ttgaacaaaa caccatcgag
    14461 catcgaatcc aactactagc cgtgtcgaat tcgccttgaa cgctggctgc ttggctgtta
    14521 gagaatggac ggaggggccg acccgagcgg ggcggaaaca attgcacgac aacttaggcc
    14581 gacaccttta atgactggcg ctcccacaag agagacttca gttcttccaa gactttacgc
    14641 tgaccggcgg agggagcaaa agcatcatcc tgactcgcag aaaaccgatg ttcaaattgc
    14701 tcgattgcgc gaccggcttc ttcgtcacag ccccagcgta acagcgtcgc aatcaatcga
    14761 aggtaggcgg gaacaatgaa tggaagatga cgttcttctt tggcgtgatc gatgacaaac
    14821 cggtaccccg agatggcggt atccatatcg gacttttcga aacagagggc ctcaagcagg
    14881 tggacgttga cgcggtccgt gtcagcactg acactccgct tccattgccc cttcaccaac
    14941 ttgcatagct cgtcgtggga caatagggga gcagctttct tggagcgctg ttgaagttgt
    15001 gccacgatat ccctcgcctc ctgccatcct ccatataacc cgatcaagaa ccccggacag
    15061 ggaactgcgc ctctacactt ttcctcaagc ggagaaaaac ctttccgatg cgcccatgaa
    15121 gacaaaaaca tatggccatg tgccctggca cacacttcac agatcgggct atcgtcgggg
    15181 accatataga cgtacatacg gacgagggac cggtcaaaca gatcaactgc ttgaattcgg
    15241 cctgctcgga cttgtaattg gtggaaaagc cataaaccca gcaaccccag aactgctgcc
    15301 aggtagattg tgccctctga atagctcatt ccctctccca tctagcctct tcgccaactt
    15361 tagtcgactc taaggatacc actcctacct ggaaacccaa gaatttcttg aaattctttc
    15421 cgcagcccct ctgaaaatta ttgtgcattc ccttcagccc ggcgtcttga acaaaccatt
    15481 cgatcgttcg gcccagaagc caatcaatgg tttgatgaga tcaatgccaa taagcgtggc
    15541 gggaagcgcc ctgacaatga cgccctgatg cctctgccca gaagcgttag tcccttgctg
    15601 gaaggtcttc cggagctgga gtggactccg gagcaggtat aaaaactgta gaccccggag
    15661 aagagccatt cttgaagggc aaatcaagga gggcataggg gttcacgcgg gcgccgttga
    15721 gcttgacgcc ccagtggagg tgcgaaccgg tggcgcggcc ggtcgccccc accttgccta
    15781 tgatttgtcc ggcctttatc aaatcgccat ctttgaccag aatttctgac aggtggaaat
    15841 acatgctgta gaaccccagg ccatgatcga cgaagatccc cctgccggag aagaaatgat
    15901 cgaccgtgag gcgcaccacc ccatcgtttg tcgccaacac ctcagtcccc tgcggtgcgc
    15961 tgatgtcttc accgttgtgg ggattcctcg gctgcccgtt catgatccgc acgcttccga
    16021 atataccgct tctccggccg ttgaccggct caacaaaaga gctgtgccat aacttcagcc
    16081 gtgagttctc ggccagagcc tgcttcacct gctcctgttc agctttccag cgcgcaagac
    16141 tcttctcgtc gagatcgacc ttgtcttttg gcagcttcaa atgttcgacg gaaaacttct
    16201 ccttggcaac caggacattg tagctgagat gcttcatccg ttcgccatgc ttgatctcta
    16261 ccgcgagttc atacgtgccg ggatcgtctt gcatatcgat cccgagcagc gcaacatagc
    16321 ctggcgactc accggatcgg aattccctga aaaatggaac cgtgcgattg agaaaagctc
    16381 ctttcacctc ggttgcctct tctgactccg gcaccttcac caccaacacc tggccctgtc
    16441 tcccgctgta ctgcccatgg atgcccttcg gtggaggagg cgtgttcgga aataactcga
    16501 cggggaagac gctggtgagc agcaccaccg ccgtgatcaa cgtgcgatcg agccgagaaa
    16561 tccgcactcg cacctgtttg aaaacgggaa taatcatcgg taaaaccgca acccggacac
    16621 ctgggacact aattcatcca cgcggcgatt gacggcactg aacggatcca tgcagagaat
    16681 cgtttcttcc caatagccgt tcaatttcag gtaggtccaa tcgaccgtat aggaccgatt
    16741 ttttgcacgg gcaaaccgga taaagtctcc tcgcaccttg gctctggtgg tctggggcgg
    16801 cgtggacatg gcccgttgca ccgcgtcctc ctccaccacc cgctcaatca atccgcgtga
    16861 ctccattaaa taatacaacc ctctcgttcg cttgacatca tgatactgca gatccagtaa
    16921 aaatacgcgt ggatcgtccc agccgcagcc cttcctgtca atgtaggatt ggatcagatg
    16981 ccgcttcgtc acccagtcga cttctcgcac cagttgcatg gggtcttctt ccagcttgtc
    17041 cagaaccgaa gcccatcgct tccagacatc gtcaaggatc gggtcatgat cctgctgtgc
    17101 caggtactcc tgggcgcgat cgagatagac acgctggatc tcgacggccg tcatttgacg
    17161 gccgtcgtcc agcttcacct ttcgctttac cgttgggtca cgcgagatct cacggatcgc
    17221 cttcacggga tcctccagtt ccatcccgtg taccgcatag ccttcctcga ccatcgacaa
    17281 gaccagcgta gccgtcccga ctttgagata cgttacgagc tcggacatat tggagtcccc
    17341 gacgatgatg tggagccgcc gatagcgctc ggcatccgcg tgcggttcat cgcgggtatt
    17401 aatgatgctg cgggaggaag tcgtggagga agacgtcttc tcgtgtatgt gctgcgcccg
    17461 ttgcgagata aagaattgcg gctttccaga aaccttcagc actttccccg ctccgctgta
    17521 gacctgcctg gtcacgaaga atggaatcag ctgttccgtt accttccaaa aatcgacgtc
    17581 gcgccgcatt aagaagtttt cgtggcatcc gtacgtgttg ccgagcgagt cggtattatt
    17641 cttaaagatg tagatttctc cggaaagccc ttcctcgcgc agccgttctt cagccgccgg
    17701 caggcacgcc tcaagcagcc gctcgcctgc tttatcatgc acgaccaggt cgagaatgtt
    17761 gtcgcactcc ggcgtggaat attcggggtg gcacccggtg tcctgataga accgcgcgcc
    17821 gttgacgagg aatgcgttgg aaggccagct gttcggaatc agcccctcga agatataccc
    17881 caacaccttc tccatcggca ggtagatccg tccgttcggg gagaagataa gaccgtactc
    17941 gttctccagg ccgaatatcc gctgtttcat aggaccaggg ggcatcatga tggactcaag
    18001 acagaataca cggcggatct cccttcttca acagaaggga aggattcatc aaaacggaga
    18061 ggctaggaaa gcagttgcct ggtttcggct ggagtatagc gacggaattt ccggttgtct
    18121 ctggtgcgat cgagcaccgc cacttccagg ctctcgagcg acagcttctg gttgcccgtc
    18181 tgttcaaggg cgcctataca aagggtcagc gcggtcttca gatctggcag ctgtgccggc
    18241 accttttctt tcaacacggt ctgcaccgcc tcggatttcc caccgatcac ggcatattgt
    18301 ttctcatcaa taatactgcc gtcgaaggag attctataca tttcgttggt atggccattg
    18361 tgcccgatct ggacgaccag gatctcgact tcaagcggtt tcatttcctg gctgaaaata
    18421 gttccaaggc tctgggagta tccgttcgcc aacgaccgag cggtgacgtc ttcccgactg
    18481 tacatgaacc ccttcaggtc ggcatgcctg atgccagcct tccgaaggtt ttcgaactcg
    18541 ctgtacttcc cggcaccggc gaacgcaatg ttgtcgtaaa tttcagaaat cttgtggagg
    18601 gtcgcactcg gattctcggc ggccagaaga attccgttca tatattccat ggcgatgatg
    18661 gacctgccct tggcgatgcc tttcttggca tactccgcct tgtcctgcat catctgctcg
    18721 ggcgagacgt aatagggcat cggcatggtt atcgctcctt agatcggcgg gtctcgatca
    18781 gactgttgta gacgccgagg atttgctgct cggacacgtc ggtaatgccg gctgcattga
    18841 caatcttcgc cgtcgggtaa atccctcgta ttagatctgg cccgccggtc ccgacgtcgt
    18901 cgtccgcggc attgtagagg gcgagaagag ccagtttcag tgcatccggc tccgccagcc
    18961 cgcggctgaa atgttcacgc atcgtattgc gcgcgtcttt gccgccggaa ccgatggcat
    19021 ggtaatcgga ctcttcatag cgaccgcccg ccaagtcgta tttgaaaata cgtccttcgg
    19081 accgcttgag gtcgaacccc acgtagagcg gcatgacgac caggccttgg aacaccatcg
    19141 gaaggtttgc tttaaccatc tggccgagct tgttggcctt tccttcgcag gaaagctgca
    19201 tcccttccag cttctcgtaa tgctccagct cggtctgaaa cagtttcgcc atctcgatgc
    19261 aggggcctgc cgcgccggca atggccatcg ccgagtagtc gtcgatctta aacacttttt
    19321 cgattcggcg ttcggcaatc tgatagcctt ctgtggctct ccgatccccc gcaatgatga
    19381 ccccctgttg atacttgatc gcgagcaccg tcgtcgcctg cggcaccgcc acgcccccac
    19441 cggttctcga cgactcaaga cgcgacggct ggttctcagc ccatcggcgc ccgccgggtg
    19501 tcagttcggg atgctgtgct gtgaggaaat cgaagaaact ggcgtcgtca tggttgggaa
    19561 gaaacggaag cttcatgatg ccttcagtcg cgccagtaag gcttctgccg tgtcagattg
    19621 gtcgagcaag tccgctgtca acgattcggt ccctcgaagc gggtccatta acggaacctt
    19681 cttgatcgtc gtattgccga cgtcgaacag caccgaggtc cagcttgcgc cgtagatcga
    19741 cttggaaaac ttcccgatgc accgtccgcg gaaataggcg cgcgtccccg gcggcggcat
    19801 aaactcggct cggctcacct cgacatcctg caccacacgc tcgatgagat ggcttcgctc
    19861 cagggtatag tagagccctt tgtcgggacg gacatcgtga tactgcagat ccatcaggcg
    19921 cacgcgcgag tcattccacc cgcagccctt gcgatccatg tacgattcca tcatctggcg
    19981 cttggccacc caatccagtt cgcgcgctaa caaccgggga tcgcgttcca atttatcgag
    20041 cacatcctcc caacggacga gcacatcctt cgtgacctga ttcaactcgt ggcaggcgta
    20101 gaaatccatt gccgtcttga gatacgcgcg ttgaatttcc aaagcggtcg tcgaccggcc
    20161 ccccgtcagt ttcaatgtgc cgctcatggt cagatcccgc gacacctgtt tgatcgcgag
    20221 caccgggtca tctaactcaa cttgaggcaa ctcgacaccc gcttcgacga gatccaagac
    20281 gatcgccaga gtcccgacct ttaaatacgt cgacaactcc gccatgttgg cgtcgcccac
    20341 gatgacgtga agccggcgat actttccata atcggcgtgc ggttcatcgc gcgaattgac
    20401 gatcgggcgc ttcaccatcg tattgaggtc gacaagacac tcgaagaaat ccgctcgctg
    20461 agaaatctga tagtccgccg aactcgtttg attctctgcg ccaaccttgc ccgctcccgc
    20521 atagatcggc cgcgtcacga agaagggcgc caacgccttc acgatccgct cgaacggtac
    20581 cgagcgggac agcatataat tctcgtggta tccgtaactg ttccccttcc cgtcggaatt
    20641 gttcttgtag aggacaaact gctcggatcc ccttacccgc gtcatttcat cgaggcactg
    20701 ggtgagaatc cgctcgctga tccgctcgta cgctacgatc tctcgcgggt tcgtgcactc
    20761 cggggtggaa tattccggat gcgcgccgtc gacatagagg cgtccgccat tggcgagcac
    20821 tttgttgagt tggcgattgt agtcggggtt cggccgttca cgctctccgt cgacttcgaa
    20881 ccctcgtgcg tcgagcagcg gattctcgtt ttcgtaatcc cacacggcgt ggggaactga
    20941 gagaccggga taatgcccga tcaccgcaag agagtttgaa atgggatccg tcgcagacgc
    21001 gtctcgggag gcgatgccga attccgtctc cgttcccatt acgcgaggag tcgtcagggg
    21061 tgtggattcc cgcatggagt taggtcttag agatagtggc ccgtcgtgat ggtttcaatc
    21121 tgacgtgact cggccggccc accgctgatc gttcgaatat gcacgatctt ttcgcctttc
    21181 ttgccggcaa ccttagccca gtcatcgggg ttggttgtat tgggcagatc ttcgtgctcc
    21241 ttgaactctt ccctgattgc ccggatcaaa tcctcggagc ggaggccgcg gccttcctgc
    21301 gcaatcgcac gcttcacggc gtacttcttg gcccgcgaga cgatgccctc gatcaaagca
    21361 ccgctggcga aatccttgaa atacaggacc tccttttcgc cgttcgcgta ggtcacttcg
    21421 atgaacttgt tttcttcgga cgtggcatac atggcatcga cggtcatgtc ggtcagatgc
    21481 tcgaccagcg ccttaacatc ccctccatgg cgctgcaatt cttcgtggtc gaacggcaaa
    21541 ttcgccccga cgtatttcga aaagatgtct ctcgcggcca cggcatcagg gcgaccgact
    21601 ttcaccttca catccagacg cccggctcgc aagactgcag ggtcgatgag gtcctggcga
    21661 ttgctcgcgc cgatgacgat cacgttccgc aggcgctcga caccgtcgat ctccgagagg
    21721 aattgcggca cgatcgtgga ttcaatatcg gacgaaatgc cggtcccgcg cgttctgaac
    21781 agcgcgtcca tttcatcgaa aaacacgatg actggtgtgc cgtccgcagc ccgctccttc
    21841 gccttcttaa agacttctcg cacctggcgc tcggactcgc ccacatactt gttgagcagt
    21901 tcaggcccct tcacgtgcag gaaataactg cgtacttcct taccggtgag atggccgagc
    21961 ttcttggcga ttgaattcgc cacggctttg gcgatcaatg tcttgccgca gccaggaggc
    22021 ccgtagagca acacgccttt gggagcgctc aagtgaaatt cggcaaacag ttccggatgg
    22081 aggaacggca gttcaaccgc atcgcgtacc tgctcgagtt cccgatgaag tccgccgata
    22141 tgggcgtaat ccacatccgg aacttcttcc aggaccaatt cttccgcttc ggacttcggc
    22201 aatttctcga tgacgcagcc ggagcgagga tcgtagagca gatggtcgcc cacactcaga
    22261 cgttcagcca gaagggggtc gccaagctcc gcgacttttt cttcatcgaa gtgcaacgtc
    22321 accagcgccc gattcccctc gagcacatcc ttcacccgga cgacttcacc ctggacgtcg
    22381 aacccacggg cttcaatgac gttgagggct tcgttcagga tgacttcctg tcccttgcgc
    22441 agcccgttcg ccttgatgga cggatgcagg cttactttca tcttgcgtcc cgacacatac
    22501 acgttgccgg tgccgtcctg attgagactc gaaaaaatgg cataggtaga tggcggagcc
    22561 gtcaactttt caacttctgc acgaagcgcc tcgatttgcg ccttggcctc ctgcagcgtc
    22621 atcgcaagtt tttcgttctg cttggtggct tggtcgagtt ggtagcggga ttggtacagc
    22681 cgacggattt cttcctccat cgactgaatt tgcacgcgga gcttttcgat ttcgcggtgt
    22741 tgttcgtcgt tccggtgaat cacgtccgct tctccctcag acaaccgctt ggtgatgtgc
    22801 ttgacggaat ctcggagaga ccggagtctg ctcggacttc ctttcggatc ggccatggca
    22861 ccacccgtga atggcgagaa gtcgttgctg aatcatgaag tccaagtctg tgcggattct
    22921 aacacccgct attttaacgg tcaacaggag gaccgattcc cgcacaggcg agctgcgcag
    22981 tcgagaagat catggatccg ccgagacggc gtccaacatc tcgcgcgcag ccgcttcaac
    23041 gtccgtcgct ccgagaatgg ccgtgatgac cgccacaccg aacgcgccgg cgcgacgcac
    23101 gtcgcgcgca cgagccggtg tgactccacc gatgccgatg accgggatcc gtacgcagcg
    23161 gcaaacttct ttgactttct ccaagccgag cggaggaccg aacgcctgct tggacggagt
    23221 ctcgtacaca gggccgagca cgacgtaatc cgctccttgg gcctccgcct gcaccgcttc
    23281 gtccagcgaa tgtgcagaga caccgagcaa acgttgttca cccaacagtc gccttgcgac
    23341 ggagaccggc aaactgttgc tccgtaaatg cacgccgact ccgtcgagca caagggccag
    23401 gtcgattcgg tcgttgatga ccaattgact ccccttctgg gtcgctaatg tctggactgt
    23461 gcgcgcgaga gccagaaggg ctctgggagc gagatcgcgt tctctaagtt ggataacggg
    23521 agaccccgcc gccagcactt tttctagaat cgcttctaac ggacgcccgt tggtctgatg
    23581 acggtccgtg accacaagaa gacgactgga gagtttacgc atcagcggaa cattgagtcg
    23641 aacgtgctga gtggcgagcg tacaggtgtt gcactcttgc tatccatagc ctcagcactc
    23701 atcactccgg acgtatggga ttagagcatg ccttctatgg ggctactcgc agtcgcataa
    23761 agctttctgg gaattctccc ggccttgtaa gccaaccgtc cggcatgcac ggcatacttc
    23821 atggcttcag ccatagcgat aggatcccgc gcgcccgcga tcgctgtatt catcaagact
    23881 gcatcggcac catattccat ggccaatgcc gcgtccgagg ccgtcccgac gccggcatcc
    23941 acgatgatcg gcaccttgac cgtttccatg atgatcttga gattgtaggg gttacgaatg
    24001 ccgaggccag atccgatcgg ggcagcgagg ggcatcacgg cgggacaccc gatatcaacc
    24061 agcttcttcg ccacaatggg atcgtcattc gtatacggaa ggacgatgaa cccttccttg
    24121 atcaggatct ttgccgcctc gatcaacccg gccgtatcgg ggaagagcgt cttctcatca
    24181 cctagcactt cgagtttgac cagatcggaa acgccggcag cccttgcgag ccgagcatag
    24241 cggacggcat cctccactgt gtagcacccg gctgtattcg ggagaatgat gtatttcttt
    24301 ggatcgaggt aatcgagcaa gttctctttg gaacggtcgg tgatgttcac gcgacggaca
    24361 gccactgtga cgacatcggc cccggacacc tcgatggcct ttttcgtctc cacgaaatcc
    24421 ttgtacttgc ctgttcccac ccatagtcga gacttaaatt cccggccggc tatcaccaaa
    24481 cgatcatcgt gcatgactac aagcctcccg ttcgccattc cgccctagcg gtgcgccgcc
    24541 gccgatgaaa ctcaaaattt ccactcgatc cccttcttta aggcctcgat gatcgaattc
    24601 cttacggtca agaatctcca agttcaactc caccgcgacc cgctccgtct tgattgcaag
    24661 ttctcgcagg agatccccga ccgtcaatcc ggcccggcaa tcacgccgct ctccattcac
    24721 ctgaatctgc attccatccg tcacagttca tcatcctagg gaaccataat tccgcttgtc
    24781 aacaaagtcg atctggctcg gtcccttgcc tctgcgccaa aggacccggc acaatagggc
    24841 cataggccta tgtatggcga cacctctgaa taaccagcaa attgccacgc tctttcggtc
    24901 catggcggaa ctgctcgccg cccagagggc caacccctat cgagtccggg cttacaggcg
    24961 agcagctgag acgctgctcg cccaggagga agatgttgcg gagatcgcca agaggaacgc
    25021 gcttgaagaa atcgacggta tcggcaagga cttatcggaa aagatcgtgg agtttctgag
    25081 gacgggtaag attcaggcgt tcgaagccct caagacgcca ttaccggatc aggtgagaga
    25141 ctgggctcga ttgcccgggc tctctgaatc attgattgcc tacctataca gccgcctcaa
    25201 catccacaca ttggatgagc ttgaacaact tgtgcggtcc catatgctca ggacccttcc
    25261 aggtttttca gggtccgagg agaaactact tcaggccatc acaatccttc gcggccactc
    25321 ttctctcaag aaggacggag ttccttaaag attttccagg tcttgagcat ctccacagcc
    25381 ttttgcaact gaacatcctg ctcaagcgct ggttcaccag aagcatccgc tgaaggcaca
    25441 gcgggcgttg tgccgttctt tggcgtcccc tcttcatgcc ccttcccatt gacggaagac
    25501 tgttcttttc ccggtgcagc tgtgaccggc ggctttgtgc ttttcgcgtc cgcgtctttc
    25561 tctcctggct tcacgtcggc cttggcgacc gtcgtcggtg cctgcggctt aacgacgata
    25621 tcgggggtga ttcccgtcga ttggatcgag cggcccttcg gcgtgtaata cttcgccgtc
    25681 gtgagtcgta gccctgaacc atcccccagc gggagaatcg tctgcacgga tcccttcccg
    25741 aatgacgtcg ttcccacaat gacggcccgc ccgtaatcct gcaatgcgcc ggcaacaatc
    25801 tcggaggcgc ttgcggaccc ttcattcacg agaataatca tgggcgaatc gtccatctga
    25861 tccttcccct tcgcaaacca ctcgtctttc ttgctttcgc ggcctttcgt atacacgacc
    25921 agttttccac tgggaaggaa ttgctccgaa acctcgaccg cggcggtcaa gagaccacca
    25981 ggattgtttc gaagatcgag aatggtcgcc tggagcttct gctctttgaa ctgcttcagc
    26041 actttcccca gatcccgacc ggtagcttcc tgaaattggg tgagcttgac atagccgata
    26101 ttgtccaaca ccttcgactt gacgctctcg atcttgatgg tatcgcggac cagcgtgaat
    26161 tggagcggat caggagaccc gtcccgctga atcgtcagat tgaccttggt cccctttggt
    26221 ccgcgcatct tctgcacggc atccatgaga gagagatcct tcgtcgactc gtcgttgacc
    26281 ttcgtaatat agtcgccggc cttgatcccg gctcgttgag ccggagtgcc gtcgatcgga
    26341 gcaatcaccg ccaggcggtt ttcttttacc ccgatttgaa tgccaacccc tccaaactct
    26401 cccttggtct caacttgcat ttccttgtac atctcgggag tcatgtaggc cgaatggggg
    26461 tcgagggttg agagcattcc gcggattgcg ccctgcacga gatctttgac cttggtttcg
    26521 tccacataat ttcgctggac ctgtgacaag acctcggaga aggttttcaa ctcttcatag
    26581 ctttcggtgg catggccggt tcgttcccat cccttgccaa tcaacacgcc ggacacgagt
    26641 gctattacga ccatgggtcc aatcatccag aatcgccgcc gtgggttctg ttccatcata
    26701 tgcattcctt tcgccttcct cgtgtcttat cgtctcgcca gccactgaag ggggtccacg
    26761 ggttccgccc cctcacgcaa ttcgaagtat agggtatttt cccccgtcat gcctgtatcg
    26821 cctgtttctc cgatcgcctg acccgatccg acttgggccc caaccgatgt cagaatcctc
    26881 gatgcatggg catagagtga gaagaagccg ttcgcatgat caaggattat aacgagtccg
    26941 tagcctttca accagtccgc atagacgacg ttccccccca tcacggctcg aatcgaactc
    27001 ccctccgccg tcttgatctc gatgcccttg cgctgcacgt aggtattgaa cgtcgggtgc
    27061 ttctgccggc cgaaatacgt gatgacgctg ccgtccgcag gccacggcaa cccccccttc
    27121 accccgcgcg gtgccagcgc gccgctcggc ggcctcgcgg cagctgctcg gcggcgattc
    27181 tccaactccc gcagcaaatt gtcgatgcgt gatgcggacc gttcgagttc ctgcagtgca
    27241 cgctcgtagg actctttttc atgcgtcagc ttcgtcaggt agaccctttt ctccttcttc
    27301 accgctttga tctcttcgag ctgcttttct gtactccgtt tgtaggccag catgccgaca
    27361 cgcgcctctt cacgctgccg ttcggcttcc tgcatgcgag ccatatcgcc cttgaaggtt
    27421 tcaagcaggt cgtagtcccg ttcagagacc gccgacaggt atcgtagtcg gcgctggaaa
    27481 tccccgtacg aatccgaggc cagcagggtt ttccaatgtc cgaagcgccc ttcaatatat
    27541 tgtacgcgca agcgcgccga gatggcatcc cggcgttcct ggacactgtc ctgcagccgc
    27601 gacagttgcg cattgatcgt ctcgatctcc tgatccttct tccgtaattt gcggttgatc
    27661 tcctggtggg cctgccgata gcgcatgtgg cgctcatcta atgtttgaat cccctggagg
    27721 atcgattccc gctttttgct tgcctcgtcc gcctgccggc gtttctcttc gatctgatct
    27781 ttcagctgtt cgagcgcctt tcgttccttg tcgatttttt cgttgatgga gtcggcccaa
    27841 gcgccggcag gcatcgacca agccagcagg ctccaaacca gcatactcat ccatcgcccc
    27901 gtagacttac tcataccttg gcctcgtcga accgcctgag cgacacaaaa ctaccggcac
    27961 aaccgagtcc caccccgatt gccaccaaca tcaagcacac ggagagcgga aaaaacatca
    28021 tgagctgttc catcccgctg aatcgtccgg tcatcctcat ctgttgcctg aataactcga
    28081 agagaagctt gagcatgccc aatgacacag cgcttcccag cccacccagc acggcaccct
    28141 ccaacaaata ggggatgcga ataaacgatt tggtcgcgcc gatgagccgc aaaatggcaa
    28201 tttcatcgcg ccgggcaaac agagtgagcc tgatcgtatt ggcaataatc gtcacggccg
    28261 ccgccgaaag gaccagtccg ataccgatcg caaccacttc gatagaccga atgaccgtcg
    28321 acagtgcgtc gatccagtct tgattataat ccaccttcac gacaccagcg agctgcccga
    28381 cccgttcagc ccatcgcttg acggcatccg gagaacgaaa cgcgggaccg agcgtcacaa
    28441 caaaggatgc gggcaatgga ttctgaccga ggccttcgag caaatgggac tccgacggga
    28501 actgcgcctt aaattccccg agtgcttgct ctttcgaaat aaaatgctgt gccgcgacag
    28561 cacggtcgcc gcgtaactgc tgttcgatct cggtgatcgt cgtggccgcc gctcgatcat
    28621 cgaggtagac cataatcttg atatcgtcct gaagccagcc cgcagccgca cgcaagttca
    28681 catacagcaa taaaaaaata ccgacacagg ccagcgtaaa cgccgtggtc agaatggcca
    28741 ccatcgtggt cgttcggttc gtccaaatgt tggcccaggc ctcccgcagg agatagaaaa
    28801 tcgatctcat acgccgaccc cctgctccgg catcaacgaa ccttgcacca gcgtgaccac
    28861 ccgccgcttg acttgcgcca tcaccgatgg atcgtgcgtc gccacgacga cggtcgtccc
    28921 ccgcgcattg atcagcttga ataactcgat gatttctccg gtcaacgcgg gatccagatt
    28981 gcccgtcggt tcatctgcca atagcacgac cggaccattg acaatggctc tcgcaatgca
    29041 gacccgctgc tgttcacccg tcgacaggct tacaggcagt tgatcgcgct tatgctccac
    29101 cccaacggcc cgcaaggctt cggtgacttt tcgtttaatg tctcccggag acaccccctg
    29161 cacgagcaag gggagcgcca cattttcgaa caccggcttc ttgggtagca ggcggaaatc
    29221 ctgaaagact gttccgacct tgcgccggag aaaaggaatc tccgacgcct tcagtgttgt
    29281 cacattcttc ccgttgacga acacctgccc ctcgtccggc cgttcggctc caatcagcat
    29341 ccgtagcagc gtcgattttc ctgcaccgct cggccccatc aacagcacaa attcgccctt
    29401 ttcgatttca agcgacacgt catttagggc gggacgacgc tcatactgtt tggtgacgtg
    29461 aatgagctga atcatcgctg tttcaccacc gtggctcgct ccgggagacg tcgaacgcat
    29521 tctggatgtg ttcgatctga ggagccgacg catccgtccc ttggagcaac acaacatcaa
    29581 accggcaggc gcgatcggtc aggtgatgct tggcaagata ttgagacgcc aattggatca
    29641 acttttcctg cttatgacga tggacggcgt ggatcgctcc cccgaaatcc tcgctcctgc
    29701 gtgccttcac ctcgacaaat accaatactt tcccatcctc cgcgaccaga tccaattccc
    29761 ccaatgtgga ccggagattc ttcgcgacga ttcggtagcc cttccgccgc aagtaacgct
    29821 ctgccgtcgc ctctccttct tgcccaaaga gtcgtctggg gtcaagcaat gtcatgctat
    29881 cgtcctctgg gattcctcct gacgagaaac cgtctgctgc tggctggaaa gatgcgcaat
    29941 catctctgcg accggcgcaa aggtcttccg gtgaatcgga cagggtccgt gcaccgcaag
    30001 ctgccgcaag tgttcgtcag tcccgtagcc tttatgggaa agaaaattgt agtggggata
    30061 gagctggtgg tactccgcca taagacgatc cctggtgacc ttcgccacaa tggatgcggc
    30121 cgcaaccgag attgacaatg catcgccctt aataattgaa cgggtgggaa tcgcgcatcc
    30181 aggcagcgct accgcgtcga tcagcagaca atccggctga ggcgacaacg ccgcaatggc
    30241 ccgacgcatg gccaggcgcg tggcttcaag aatgttcagc cggtcgactt cttcctggct
    30301 cgccgagccg attcctatgc caaccgcccg ctcgctaata atttgataca ggcgttcgcg
    30361 atctgattca gatagctgct tggagtcgtc gcagccgatg agatgacagc ggctcggcaa
    30421 gacgaccgca gccgcgacga cggggcccgc gagcgggccc cgtccggctt cgtcaagacc
    30481 ggcgatccgg cggtagccgc atcgcctggc ttccaattca aattcgtcgg tgggtcccac
    30541 gaagttgcct acagtgtgcg tgccggcacg cacgaccctc gtggtcgaca gaacaggaaa
    30601 gaattaggac ttcgcggctg ccgccgcttc ttccggcgca tccgccgact cagtcgcgcg
    30661 cacggacccg ggctgtgctt tgccctctcc cacaaactcc cgatcttcga ccctggagaa
    30721 cttgcccttc ttcccgcgca ggtaatagag cttggcccgg cgcacttttc cttggcgcac
    30781 tacgtcgatc ttcgtgacga tcggcgaatg aacagggaat attcgctcca cgccgacgcc
    30841 gtaagaaacc ttgcgcacag taaacatctc cgtattgagg cttccctttc gggcaatgac
    30901 tgccccttca tagacctgaa tacgttcttt ttctccttcg atcaccttga cgtggacacg
    30961 aaccgtgtcc ccgatctcaa agtgaggaac cgacttcttc tgcaacgacc gttgaacccg
    31021 ctctaactga ttcatgacct cacccctcct ccccacatcg aaccggcgtg ccgcgcacgc
    31081 cttcgcgaat aatgtcatcc aacaattgtc tgtcctcaac ggtaaaaacc cggtccttga
    31141 gcagatcggg ccgcttctca taggtcgccc gcaacgcttc cttcctgcgc caaagacgaa
    31201 ttgcttcgtg gtgacctgaa agcaacacct ccggaaccgt catgccacga acgtccgccg
    31261 gtctggtgta gtgcggatac tccagcaacg aatccgtaaa cgattcctcg acgatcgatt
    31321 cgggatcccc caacacgccg ggaatcagtc gcgctgccgc gtcgatgaga atgagcgccg
    31381 gcaactcgcc tcccgtcaag acatagtctc cgaccgatac ctcctccggc tggagcgcca
    31441 cccggacccg ctcatcgatt ccttcgtaat ggccgcagag gatcacaaga cggcgcgact
    31501 ccgctgccag gtcctgtgca taaacctgcg tgaacggacg cccgtgcggc gatggcagca
    31561 acagccgaat ctgttcgcca gcctgttcat aaccagcctt aatttgctcc accgcacata
    31621 ggatcggctc agctttcatg accataccgg ccccgccgcc atagggcaca tcgtcggcca
    31681 ccttgtgctt atccaacgtg tagtcccgca gattatgcac cttcacgtcg agcaaaccct
    31741 tttcttgcgc gcgcttcagg atgctgctcc cgagcaccgg tgccaccatc tcaggaaaca
    31801 aggtaatgac gtcacagcgc atggcgatcc tcgactaatc cctcgatccc gcgcaccgtc
    31861 atccgccgct gtaccagatc caccttcacg acaaaattct ttgccgctgg aatgagcagc
    31921 tctcggtcat tcttccgcac gacaaatacc tggtggcctg gcagctccca aatcgtctcg
    31981 acgagcccca gttcacgtcc gtcttcatcc accactgtca ggccgatgag atcacactcg
    32041 tacagtgtat tccccgtgcg agccgagggc tcacatcgag ggacttggat caatccgccg
    32101 cgaagcgtcc ccgcttcttc cggcgtcgtg atgcaggcga actgcacgat atacgtggga
    32161 ccggccctgc gcacatgcgt gacagtccga tcgaccgtcc gccccgtcaa ctccatcacg
    32221 ctgacttcac ccagactatc gaaccggccc ggcacatcac tcaaggatcg aaccttcacc
    32281 gcgccgtgca cgccgaaggg ccgttcgatc tttccgatgg tcacgaggtc tgcttgaacg
    32341 gtggtcacga tttaggcctt cttcttcttc gccgccttct ccgcctcaaa ctgcttccac
    32401 aggcccgaac gacgaagcag cgtcttgacg gtcaccgttg gctgagcgcc ctggtgaagc
    32461 caagagagca cgcgctcctg tttcaattcc ggcaagcctg cttccttcaa gggatcaaaa
    32521 atacccaaaa tctctaagaa acgtccgtcg cgaggcttgc gggaatccgc cgctatcacg
    32581 cgatagaccg gtcgcttatg tcgtcctgtc ctcgtcaacc ttaaatgaac ggccactgtc
    32641 atcctccttc tctacgagac acacctgccc ggggtccctg atttcgaact tacattgaac
    32701 gcaagagctg cgcgagttgc cgacgccctc ctgctcctgt catcaccttg gcgatctttc
    32761 gcgccgatag gaactgcttg atcaatcggt tcacttccat cacgtttgtc ccgctgccac
    32821 gcgcgatgcg tttcttccgg ctcccgttga ccatggtgtg atcgcgacgc tcgcgggccg
    32881 tcatcgaatc gatgatcgcg aggactcgct tcatttcccg ttccggcatg tgcccgtcca
    32941 tggcgccttt taacttttgg ccgcctggca acattcccag aatttgatcc aacgagccga
    33001 gcttgctgac ctgtcccaac tgagctcgaa aatcctctaa cgtaaacgtg ctgctggtca
    33061 accgcttttg agcctcttcc gcctgctcgc gcgtaaacgt ctcttgcgcc ttctcgatca
    33121 gggaaagcac atcgcccatt cccaggatac gcgaagccat ccgatccgga tgaaacagct
    33181 caagcgcatc cagcttctct ccaatgccaa gaaacttgat cggtttgccc gtgacggccc
    33241 tgatggacaa cacggcgccg ccccgcgcat caccttccac cttggtcagg atgattccgg
    33301 tcagccccac tcgttgatca aactggctgg ccatcgtgac ggcatcctga cccgtcatcg
    33361 catccgccac cagcaacact tcgtggggct gcacagcgga cttgaccgcg accaattccc
    33421 ccatcaattc gtcatcgatg tggaggcggc caccggtatc cagcacaaca agatcaaatc
    33481 cctgatcgcg gcctcgctcg acaccggcct ggcagattcg cacgacatct gtttgcgaag
    33541 cccctgcctg atcgagtcga tgcacatcga tcccgagatc gcgtcccaaa ctggccaatt
    33601 gatctccggc cgcaggacgc cggggatctg ccgcgaccag gagaactcgc ttaccttgat
    33661 tcttgaatag ttttgccagc ttgccgcagg tggtcgtctt tccagcgcct tgcagaccaa
    33721 ccatcatcac gacggtcggc ggtgtcgctg caagcgaaag accagccctt tcgccgccca
    33781 tcatcgcacg caactcgtcc cagacgacct tgaccacctg atggcccggc gtcagactct
    33841 gcaatacatc ctgcccaacg gccttttcgc gcacccgctc gatgaaatct ttgacaatct
    33901 tgaagttgac atcggcttcg agtagagcaa gacgaacttc cttcaacgct tccgcgatgt
    33961 tctgctcggt gaggacaccg ctaccacgga gcttcttcag gatcttttca aatttttcgc
    34021 tgagagaatc cagcatcaca caccaaagaa aaaggcaacc gaagccttac atccagatct
    34081 ccgatcgcct cgaaaaatct agcaaaagag catcgggcag tctaaaggag gcccctccag
    34141 aagtcaaggg agcctctatc aactttgagt tgacacacta ggggtcattc gatatggtcg
    34201 tatcggtcat tcggcgaggc tggtcaacga gttatgaggc agtcggcgtg agaaaatccc
    34261 cctgggtctt actgcttttc ctcctgatcg gcggattact cggcggaatt ctaggggaag
    34321 tccttcaagt tatggccccg aagggaggca cgatccaagc gttctttata accgctgtca
    34381 cgcccggtat cagcccaccc ctgacgatcg atcttattct tctgaagatc acgcttggct
    34441 ttagcttgaa aatcagcctg ctgagcgtcc tcggcatgtt cctcgggatc tacctctaca
    34501 agaacgtcta aacttctgac ttcagttcca aatctttcag cttcaaggta cggatgcggt
    34561 ttcgtatggc cgccgcacgc tcaaaatcca gctccttggc cgccgccttc atctcaagct
    34621 ccagccgctt gatcagttgt tcgcaggctt cctctgttcc gtactcgtgc tgcgattcgg
    34681 cagccagcgc taattgctcc ggttcaacgt aggcatcggc atactccaac gttggtatct
    34741 ctttcttgat actcgccggc acaatcccgt gagccgcatt gtagtcggcc tggatcctgc
    34801 gcctgcgaac cgtttcgtca atcgccgctt tcatcgagtc cgtcacgacg tcgccgtaca
    34861 gcgtgacccg cccccgcaca ttgcgcgcgg cccggcctgc tgtttggatg agcgaccgga
    34921 aggagcgcag atatccctcc ttatcggcgt cgagaatcgc gaccagactg acttcgggaa
    34981 tatccaatcc ttcgcgtagc aggttgattc ccacgagcac atcgaagact ccgcgccgaa
    35041 gatcgcggac gatttcggcg cgttcgagtg tcttgatgtc tgaatgaaga taccgcactt
    35101 tgattcccag ctcgtgatag tactcgctca agtcttccgc catgcgtttg gtgagagtcg
    35161 taacgagcac ccgccccccc gccgaaacct ctgcccgcac ctgcccgagc agatgatcca
    35221 cctggccttt cgccggctgg acgtcgatca ggggatccag caagccagtc ggacggataa
    35281 tctgttcgat cacttctccg ttcacatgct ccaactcgta tggaccgggg gtcgccgaga
    35341 cgtaaaccac ctgattgatg cagcgctcga actccggaaa tttgagcggt ctgttatcga
    35401 cggcagaggg caggcgaaat ccatactcca ccagggtacg cttgcgggaa tagtctccct
    35461 catacatccc gccgacttga ggaatggtgg cgtgagattc gtccacgatc aacaaaaaat
    35521 ccttgggaaa gtagtcgatg agcgtcggcg gcggctcccc cggagcccga ccgcttagat
    35581 gacgcgagta gttttcgatc ccgtggcaat agcccatcgc acgaatcatc tccaggtcga
    35641 acttcgtccg ctgcgcgatc cgctgcgcct cgacaagctg gttgtgtttc ttgaaatagg
    35701 cgacacgttc atcgagctct tcttcgatgc cggtgatcgc cttctcatag cggtctggag
    35761 cgatgagata gtgcgtattg ggatagaccg cgactttcgg gagtttcttc agggacttgc
    35821 ccgtcagcgg atcgatttca tggatggcat ccaccacatc gccgaacaac tcgatgcgga
    35881 ccgagattgc ctcattcgac gcgggaaaga tctcgatgac gtcacctctt gcacgaaacg
    35941 tcccgcgatg aaaatccacg tcattgcggg cgtattgaat ctcaacaagc ttggccaata
    36001 ttttttcccg tcggatttcc atcccttctt cgagatacac caccatcccg tgatagacct
    36061 cgggcgagcc caggccatag atgcaggaga cggatgaaac aatcaccacg tcgtttcgct
    36121 ggagcaacga cgtcgtcgcg gcatgacgca tctggtcgat cgcatcgttg atcgacgaat
    36181 ctttcgcgat gtaggtatcg gtctggggaa tgtaggcttc aggctggtaa taatcgtaat
    36241 agctgatgaa gtattcgacc gcgttgtggg ggaagaactg tttgaactct tgatagagtt
    36301 gccccgccag cgttttgtta tgaaccagca cgagcgtggg tttctgaacc cgctcaatca
    36361 cgttggccat ggtaaacgtc ttgccggagc cagtcacacc gagtagcgcc tggtgtttca
    36421 gaccggcttc gatgccggct acgagcttgt cgatagcctg gccttgatct ccacacggct
    36481 tgaagagagc ttcgagtttg aactgcggca tgagatgacc ttccgtccga tactgtacca
    36541 cgcagggatg gacccgttca cgaccgacta caagacaaga cgggcaggcc ctcgagggcc
    36601 tgcccgtctt ccgtacagga atgcgccgcg tgaactccta aatcagcgac caaatcgtct
    36661 cggcccgccg gatgacgccc cgtgccggcg gaacgactct ccgctgccgt tctcgtgaaa
    36721 ccgtctgttg gtggaatccc cgtgccgagg cggtcgcgga cgcctcgctt gttggtcatc
    36781 cgccggagcc accgcaaccg gaggcatcgt tgcaaaggct ggaagtggaa gcttcttggt
    36841 ctgaatcctc agctgtcgca ccatgcggag atactcccgc tcctc
//
LOCUS       DATIFR010000056         1547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_609877, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000056 DATIFR010000000
VERSION     DATIFR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609877"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /gene="aprB"
                     /locus_tag="VLD60_03390"
     CDS             <1..424
                     /gene="aprB"
                     /locus_tag="VLD60_03390"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HSB44026.1"
                     /translation="PSDIMHIDKVTRRAYNIEPNMCWECYSCVKACPHQAIDCRGYAD
                     FAPLGHSVRVKRDEEKGVIAWRIKFRNGKKDFELLAPITTKPWGTGQPQLRDVPSPSQ
                     EQRDSQLLYNEPKYIRLDDGDIHTLESAGLKMKEGVYY"
     gene            424..>1547
                     /gene="aprA"
                     /locus_tag="VLD60_03395"
     CDS             424..>1547
                     /gene="aprA"
                     /locus_tag="VLD60_03395"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HSB44027.1"
                     /translation="MAYETIVEDNIDILVAGAGLGGTGAAFEARYWGQDKKIVIAEKA
                     NIDRSGAVAQGLYAINCYMGTRFGENNPEDHVRYARIDLMGMVREDLLFDMARHVDSA
                     VHQFEEWGLPLMRNPKTGAYQREGRWQIMIHGESYKPIVAEAAKKSADKVFNRICVTH
                     LLMDEAKENRVAGAVGFNVRTGNYHVFKSKTVIVGAGGASNIYKPRSVGEGAGRVWYA
                     PWSSGSAYGLMIGAGAKMTQMENRIVLARFKDGYGPVGAYFLHLKTYTQNGLGEEYES
                     KWWPQLQEMVGKEYLDPEASHRTHRPIPTCLRNHALISEVNAGRGPIHMVTMEAFQDP
                     HLEEIGWHNFLGMTVGQAVLWAATDVDPKYENPELTTSEP"
BASE COUNT          343 a          443 c          466 g          295 t
ORIGIN      
        1 cccgtcagac attatgcata tcgacaaggt aacccgtcgc gcctacaaca ttgagcccaa
       61 catgtgctgg gagtgctact cctgcgtcaa ggcctgcccg catcaggcga ttgactgccg
      121 cgggtatgca gacttcgcgc cgctgggcca ctcggtacga gtgaagcgcg acgaggagaa
      181 aggtgtcatc gcctggcgca tcaagttccg caacggcaaa aaagatttcg agctgctggc
      241 gcccatcacg accaaaccct ggggaacggg ccagccgcaa ctacgtgacg tgccgtctcc
      301 aagccaggaa cagcgcgaca gccagctgtt gtataacgag cccaagtaca ttcgtctcga
      361 tgacggtgat atccacacgc tcgagtccgc tggtctgaag atgaaagaag gagtgtacta
      421 ctgatggctt acgagactat cgtcgaagac aacattgaca tcctggttgc tggtgctggc
      481 ctcggtggca ccggtgcggc ctttgaagcc aggtactggg ggcaggacaa gaagatcgtc
      541 atcgccgaga aggcgaacat cgaccgttcc ggtgcagtcg cacagggcct gtacgcgatc
      601 aattgctaca tgggcacccg tttcggcgag aacaatccgg aagaccacgt gcgctacgcg
      661 cgtatcgacc tgatgggcat ggtccgtgag gacctgctgt tcgatatggc tcgccatgtc
      721 gactcggcgg tgcaccagtt cgaggaatgg ggcctgccgc tgatgcgtaa ccccaagacc
      781 ggtgcctatc agcgtgaagg gcgatggcag atcatgatcc acggtgagtc ctacaagccg
      841 atcgtcgccg aggccgcgaa gaagtcggct gacaaggttt tcaaccgtat ctgcgtcacc
      901 catctgctga tggacgaagc caaggagaac cgcgtggccg gtgctgtcgg tttcaacgta
      961 cgtaccggta actaccacgt attcaagtcc aaaaccgtca tcgtcggtgc cggcggtgca
     1021 tccaacatct acaagccgcg ttcggtcggt gaaggtgccg gtcgtgtctg gtatgccccg
     1081 tggtcatcgg gctcggccta tggcctgatg atcggcgccg gcgccaagat gacccagatg
     1141 gagaaccgta ttgtactggc tcgattcaag gacggttacg gtccggtggg tgcctacttc
     1201 ctgcacctca agacctacac ccagaatggt ctgggtgaag agtatgaatc caagtggtgg
     1261 ccgcaactgc aggagatggt cggcaaggag tacctggatc cggaagcttc gcacaggacc
     1321 catcggccaa tcccgacctg tttgcgtaac catgcactga tcagtgaggt caatgccggt
     1381 cgcggtccga tccacatggt cacgatggaa gcgttccagg acccgcatct tgaagaaatc
     1441 ggctggcaca acttcctggg tatgaccgtc ggccaggccg tgctgtgggc ggccaccgac
     1501 gttgacccga aatacgagaa tccggaattg accacctccg agccgta
//
LOCUS       DATIFR010000057         4190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1759984, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000057 DATIFR010000000
VERSION     DATIFR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759984"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VLD60_03400"
     CDS             complement(<1..897)
                     /locus_tag="VLD60_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44028.1"
                     /translation="MKLDDHPTVQLLRRTHPEQSFPVSASTLDAVWLRTICLDAGAAD
                     VGFVELGRPALADQRDDILRIFPQAQSLISFVVRMNREPIRSSARSVANLEFHHVGER
                     VNDTARTIVAALEARGVRALNPSMGFPMEMDRFPGKIWVISHKPIAVQAGLGQMGIHR
                     NVIHPKFDNFVLLGTIVLDAKISAYDVPITYNPCLECKLCVAACPTGAIGADGHFDFS
                     ACYTHNYREFMGGFTSWVEQVASSRTARDYRKQVSDSESASMWQSLSFGANYKAAYCL
                     AVCPAGEEVIAPYLVNSKRHIEE"
     gene            complement(965..1549)
                     /locus_tag="VLD60_03405"
     CDS             complement(965..1549)
                     /locus_tag="VLD60_03405"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB44029.1"
                     /translation="MPRPKEFNPDEALEKAMQVFWHKGYEATSMEELLTAMDINRGSL
                     YATFGDKRELFLKAMDRYCSGGGIGNRISILNEPGPAIPLIRRFIGAMLEFGLSDPLR
                     RGCMITNTVMELASHEKDIARRVAGRLQMAEEAFFKLLTRARREGELANDKDPRALAR
                     VLVTMMQGTIVMIKAGTPADIVKQTAKTALSILD"
     gene            1966..2514
                     /locus_tag="VLD60_03410"
     CDS             1966..2514
                     /locus_tag="VLD60_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44030.1"
                     /translation="MQQVTRLIRERGVAQGGTAEFVWTGPEGELRQTARVAQCQPCSY
                     QMIDHVGGQAIVIETGHAIALDRNAGGVRLLMSRAPEAGQYIEIHTAPVLGRRAAYLF
                     EVWWTKLFRLESKGGRYVAGCCRTFGPCNYFQFFLLFPDCHELSQQRHCAAAPYEVAR
                     AKVYPPPSSLFLFPLLVLWLVP"
     gene            2556..3014
                     /locus_tag="VLD60_03415"
     CDS             2556..3014
                     /locus_tag="VLD60_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44031.1"
                     /translation="MSGYRLGGLLVIALMLVSSAAQAQGIFDGILDRAKDSAEQKARE
                     RTNQRIDQTVDKAINKTEETVQCVATDQECLKRAKEQGKQVTVVNAPAASDSVKCVAT
                     DTNCLKQAKAQGKKVEIVEETELDMLRCSVSDADCLKRAKTMGKKVEIID"
     gene            3138..>4190
                     /locus_tag="VLD60_03420"
     CDS             3138..>4190
                     /locus_tag="VLD60_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSB44032.1"
                     /translation="MTSFAPLRSRFSNLTAPIAGGLSLLVGALVLVGWSIDNTALQSF
                     IPGFVSMKPNAALGFALLGIALSGFSLQQPSAFSRWLAIGAAGAVTAIGALTGLESLL
                     SWNPGFDEWLFRESSALAGAWAPGRMGLNSAMALVLLGSALLMQLKKEHAWAFLCDGL
                     VIGAMLLGFIASLGYLYDERFLYGIGRFNPMAPHTALTLLGVGIGILSLHPGRGMMAV
                     VTSEGAGGYMLRRLLPVALLVFPAIGWIRLYGERQGFYGTAEGLAIFATLATATMFLL
                     LWWIARSLNDADRRRGQTAAALGQSEQRMRLALFAVDHAGDGIFWADMKQRLIYANDA
                     ARRSLGYNSDEIKALTI"
BASE COUNT          847 a         1151 c         1252 g          940 t
ORIGIN      
        1 ttcttcgatg tgacgcttgg agttaacgag atagggggcg atcacctcct cgcccgcagg
       61 gcacacggcg agacagtagg cggccttgta gttggcgccg aatgagaggc tttgccacat
      121 ggaggccgat tccgagtcgg acacctgttt gcgataatcc cgcgccgtgc gactcgaagc
      181 gacctgttcc acccaactgg taaaaccgcc catgaactcg cgatagttgt gcgtatagca
      241 ggccgagaag tcgaaatgtc cgtctgcacc aatcgcaccg gtcgggcagg ccgccacgca
      301 gagtttgcat tccaggcagg gattataggt tattggaaca tcgtaggcgc tgatcttggc
      361 atccaggacg atggtaccca agagcacgaa attatcgaat ttgggatgaa tcacgttgcg
      421 gtgaatcccc atctggccga gacctgcctg gaccgcgatc ggtttatgcg agatgaccca
      481 gatcttgccg gggaagcggt ccatctccat ggggaatccc atcgatggat tcagggcgcg
      541 caccccgcgg gcctcgagtg ctgcgacgat cgtccgggcg gtgtcgttca cacgctcgcc
      601 aacgtggtgg aactcgaggt tggcgactga tcgggccgag ctacgaatcg gctcgcggtt
      661 cattcgcacg acgaaactga tgagggactg cgcctgcggg aaaatccgga ggatgtcgtc
      721 gcgttgatca gccagtgccg gtcgtcccaa ttcgacgaag cccacatcgg cggctcccgc
      781 atcgagacag atcgtccgaa gccatacggc atccagtgtg ctcgctgaaa ccgggaagga
      841 ctgttcgggg tgtgtccggc gcagcagttg aacggtcggg tggtcatcca gtttcatgga
      901 agtccctcca gccatcacgt gtgaacggcc cttccgatat gaaccccaaa aaagagatgg
      961 ccgttcagtc caggatcgaa agcgccgttt tagccgtctg cttcacgata tccgccggcg
     1021 ttccagcctt aatcatcaca atcgtgcctt gcatcatcgt gaccaggact cgtgccagcg
     1081 cgcgcgggtc tttatcgttc gcgagttctc cctctcgcct cgcgcgcgtc aggagtttga
     1141 agaaggcctc ttccgccatc tgcaatcgcc ccgccactct tcgcgcgata tccttctcgt
     1201 gcgaggccag ttccatgacg gtgttcgtga tcatgcagcc gcgtcgcagg ggatctgaga
     1261 ggccgaattc gagcatggcg ccgatgaaac ggcgaatcag cggaattgca gggcctggtt
     1321 cgtttaagat agagatccgg ttgccgatgc cgccacccga gcaatagcga tccatcgctt
     1381 tcaagaacag ttctcgtttg tcgccgaagg tggcgtagag actgccgcga ttgatgtcca
     1441 tcgcagtgag tagctcttcc atcgacgtgg cttcataacc cttgtgccag aagacctgca
     1501 tggctttctc gagtgcttcg tctggattga attctttggg gcgcggcatg aggttatctc
     1561 aattgagaaa tctagagtac tcaattatgg aacgatcagt caagaataac ttttgccctc
     1621 ccctggtgca agaccgggcg aatgggttat gagccaggtg atgacgagat tcatagagaa
     1681 ccttgcttcg gtcgggcttc aggggcgaag gtagatcctt ccattgtgta ggccaaacag
     1741 tacagttgcg agaggcctct gaaaacgcgg atatctgcag cgattcctgc ttgaacctga
     1801 tgtgagtacc cttcgattcc cctctgccgt ggcgagaaga ggttttgtga aggagcctgc
     1861 gatcataagg aagggtgtct tgagtaacag gtacgggcat tgatcagtga agtcacatac
     1921 tacacttgct ggctcggagg tggacgatgg ctgaacagta tacatatgca acaggtcact
     1981 cgattgattc gtgaacgagg ggtcgctcaa ggggggacag ccgaatttgt atggacggga
     2041 ccagagggtg agctacggca gactgcgaga gtggcgcagt gccagccatg ctcctatcaa
     2101 atgatcgatc atgtaggagg gcaggccatt gtcatcgaaa caggtcatgc cattgcgctc
     2161 gatcggaatg caggaggggt gcgtttgctg atgtctcgcg ctcccgaagc cggtcagtac
     2221 atcgaaatcc atacggcgcc ggtccttggc cggagggccg cctatctctt cgaagtgtgg
     2281 tggacaaagc tctttcgcct cgagtcgaag ggcgggcggt atgtagccgg ttgttgtcga
     2341 acatttggcc cctgcaacta tttccaattc ttccttctgt tcccggattg ccacgaattg
     2401 tcgcagcaac gacattgcgc agctgccccc tatgaagttg cgagggcaaa agtgtatccg
     2461 ccgcccagca gtctttttct ctttcccttg cttgtgctat ggttggtccc ctagagtgtt
     2521 tggacatcct gtttcgagtg aaaaggaggc gcatcatgtc tggatacagg ctcggagggc
     2581 tcctcgtgat cgcgctgatg ttggtcagct ccgccgcgca ggctcaaggg attttcgacg
     2641 gcattctgga tcgtgccaaa gatagcgccg agcagaaggc ccgcgagcgg accaaccagc
     2701 ggatcgatca aaccgtcgac aaggcgatca acaagacaga ggaaacggtt cagtgcgtgg
     2761 caacggatca ggagtgcctt aagagggcaa aagagcaagg caaacaagtc acggtggtga
     2821 acgctccggc agcatccgat tccgtcaagt gcgtggcgac ggataccaac tgtctcaagc
     2881 aggccaaggc ccaaggcaag aaagtcgaga ttgtcgagga aactgaactc gatatgcttc
     2941 gttgttctgt ctccgatgcg gactgtctga agcgggccaa aacgatgggg aagaaggtcg
     3001 agatcatcga ctgacggcct ttcccggttg tttcttgagt tgatccccgt tgtcctcgat
     3061 agcccggcga tctcttgaca aacctcccgt ttcaatccat tctgcctgcg ctagctttcc
     3121 ttcatacgcg agttgacgtg acttcatttg cgccacttcg ttcgcgtttc tccaatctca
     3181 cagctccgat tgccggcggc ttgtctcttc tggtaggggc gctggtcctc gtcggctgga
     3241 gtatcgacaa caccgctctg caaagtttca ttcccggctt tgtttcgatg aagccgaatg
     3301 ccgctctggg gttcgcgttg ctcggcatcg cgctctccgg attctcgctt cagcagccat
     3361 ccgcgttcag ccgttggctg gccattggag ctgccggagc cgtgacggcg atcggagcgc
     3421 tcaccggtct cgaatccctg ttgagttgga atccggggtt cgatgaatgg ctattccgtg
     3481 aatcgtcagc cctcgccggg gcatgggccc ccggccgaat gggattgaac agcgccatgg
     3541 ctcttgtgtt gcttgggagt gcgctcctca tgcaattgaa gaaggagcac gcgtgggcct
     3601 tcctctgcga cgggctcgta atcggcgcca tgcttctcgg cttcatcgcc tcgctcggct
     3661 atctctatga cgagcgcttc ctgtacggaa tcggtcgatt caatcccatg gcgccgcata
     3721 cggcgctgac cctcctcggc gtgggaatcg gaattctgag cctgcaccct ggccgcggca
     3781 tgatggcggt cgtgacgagt gagggagccg gtggttacat gctcaggcga ctgctgccgg
     3841 tggccttgtt ggtgtttccg gccatcggct ggattcggtt gtacggagaa cgtcaggggt
     3901 tctacggcac tgcggaaggg cttgccattt ttgccacgct ggccacggca acaatgttcc
     3961 tgttgctctg gtggatcgcg aggtcattga atgacgccga tcgacgacgg ggacaaactg
     4021 ccgccgcact cggccaaagc gaacaacgaa tgcgactggc gttgttcgct gtcgatcatg
     4081 ccggcgacgg aatcttctgg gccgacatga agcagcgcct catctacgcc aacgacgcgg
     4141 ctcgccgttc actggggtac aattccgacg aaatcaaagc tctcacgatc
//
LOCUS       DATIFR010000058         1241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1953133, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000058 DATIFR010000000
VERSION     DATIFR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953133"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..>1241
                     /gene="rnd"
                     /locus_tag="VLD60_03425"
     CDS             71..>1241
                     /gene="rnd"
                     /locus_tag="VLD60_03425"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HSB44033.1"
                     /translation="MAATPPSHNSYITSRRELESLCERLQSSTRLALDTEFVGEDSFV
                     PKLELIQVAAQDISAVIDFPAVQADGSLARFWELLCDPGIEKVVHAGRQDLDLFAIHA
                     GQIPKPFFDTQIAAAMLGFGAQVAYANLVQRIHGTKLAKAHTFTNWSARPLSRDQIAY
                     AAEDVQFLLPIHAHLHKRLHALGRVEWVREEFSRLETSVGEKSKEPLDRYQRIRGWDS
                     LKPRQAAILRELAAWRETEARRRNVPRGRVLRDEVLLQLARHPPRTVEELRGLRGMHG
                     SEIDRNGEQLIAVINTALALPPSGWPEVPRERKPEPESIGLVELLQAVLKARAAEAEI
                     APTLLATSADLQALVDAKDKKATVDLPLLRGWRRELAGNILLQVLEGTVMVSVDPA"
BASE COUNT          275 a          370 c          342 g          254 t
ORIGIN      
        1 atacttgagc ctgagcaata ccgaaccgat gcgcccgcca gatcgtccat atagcccagc
       61 cgagtaacca gtggcagcca cacctccttc tcacaacagc tacatcacga gccgccgcga
      121 acttgaatca ctttgcgaac gtcttcaatc aagcacacgc ctggccctcg atacggaatt
      181 tgttggagaa gacagttttg tcccaaaact tgaacttatt caggtcgccg cacaggacat
      241 ttcggccgtc attgattttc ccgccgtcca agccgacggt tcactcgcac ggttctggga
      301 gcttctttgt gatcctggga tagagaaagt tgtccatgca ggccggcagg atctcgatct
      361 cttcgccatc cacgccggac agatccctaa accgtttttt gacacgcaga tcgccgccgc
      421 aatgcttgga ttcggcgcgc aagttgccta tgccaatctc gtccaacgaa tccatggaac
      481 caaactggca aaagcccata ccttcaccaa ctggagtgcg cgtccgctct cacgagacca
      541 gattgcgtac gcagccgagg atgtccaatt cctcttgccg atccacgcgc atcttcacaa
      601 gcggctgcat gcattgggtc gtgtcgaatg ggtccgggaa gagttttccc gcctggaaac
      661 ctcggtgggg gagaagagca aagagcccct tgatcgctat caacggatcc gcggatggga
      721 cagtctcaaa ccgaggcaag ctgcgattct gagagagctc gccgcctggc gggaaaccga
      781 agcacgccgc cgcaatgttc cgcgtggccg tgtcttgcgg gatgaagttc ttctgcagct
      841 cgcgcgtcat cctccgcgga cggtggagga actgcgcgga cttcgtggca tgcacggctc
      901 tgagatcgat cggaacggag aacaactgat tgctgtgatt aacacggcat tggcccttcc
      961 accctctggc tggccggaag tcccccgcga gcggaaaccg gaaccagagt cgatcggatt
     1021 ggtcgaactc cttcaggccg tattgaaagc acgcgcggcg gaggccgaga ttgctccgac
     1081 attgctggca accagcgcgg atttgcaggc gctcgttgat gccaaggaca agaaagcgac
     1141 tgtggacctc ccgctcttac gtggatggcg acgagaactt gccggaaata tcctccttca
     1201 agtgctggaa ggcacagtga tggtctcggt agatcccgct t
//
LOCUS       DATIFR010000059         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1379218, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000059 DATIFR010000000
VERSION     DATIFR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379218"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VLD60_03430"
     CDS             <1..497
                     /locus_tag="VLD60_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB44034.1"
                     /translation="TPLEFSACDLSTITSNVFRSLGKMAQERNVRLITEDLELLPLIV
                     ADPRRLYNAFFNLINNAIPEVPPGGSVTVRGQTSTPEGSVVVSVIDTGRGMPPEVRDR
                     LFTKRVISTKPGGTGLGTKIVKDVVDAHGGTIWVESELGVGTTIHIRLPHAPKDSTRS
                     SAIV"
     gene            complement(553..945)
                     /locus_tag="VLD60_03435"
     CDS             complement(553..945)
                     /locus_tag="VLD60_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44035.1"
                     /translation="MLMGRVLIVDDEEHVRKTVGLALKQGGYEILEAVDGDQAIAMIQ
                     SSPTGFSVHAIICDIDLPKVNGHDVVAFIRAKLPSVPVIVLTGQPDVQGAASLFKQGV
                     VDYLIKPAQAQTLLDSVRRAIGEQALMG"
     gene            1399..1797
                     /locus_tag="VLD60_03440"
     CDS             1399..1797
                     /locus_tag="VLD60_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44036.1"
                     /translation="MHGTILIVDDDAGIRDTVRLVLAREGYGILTAPDGETAMQLMAM
                     ADTSAKVCALLCDLEMPHMGGKALIEHFRKHFPDIPIVVLSGASDTVFLEGIVQEGVS
                     DWLRKPVSRETLLDKVRTATNLFALRQQHR"
BASE COUNT          461 a          627 c          567 g          481 t
ORIGIN      
        1 gcacgcccct tgagttttcc gcatgcgatc tgtccactat caccagcaac gtgtttcggt
       61 cgttgggcaa gatggctcag gaacgaaacg tcagattgat caccgaagac cttgagttgc
      121 tgccgctgat cgtcgccgat ccgcgacgcc tctataacgc cttctttaat ttgatcaaca
      181 acgcgatccc tgaagtcccc ccagggggct cggtcactgt gcgcggacaa acgtcgaccc
      241 ccgaagggtc tgtcgtggta tccgtcatcg atacgggccg cgggatgccg cctgaggtta
      301 gagatcggct attcaccaaa cgcgtcatca gcactaagcc gggcggcacg ggactcggca
      361 cgaaaatcgt gaaagacgtg gttgatgctc atggggggac catctgggtc gagagtgaac
      421 tcggagtggg aacgacgata cacatccggc tgcctcatgc gccgaaagac tcgactcgct
      481 cctcagcgat cgtctgaaat aaatgggggg tccttggagg gccgatgata gcggcaatct
      541 tgtgcaggag tactagccca tcaacgcctg ttcaccgatc gcccgcctga ccgaatccaa
      601 tagcgtttgt gcctgggccg gcttgatgag atagtccaca acgccttgct tgaacaggga
      661 agccgctccc tggacatcgg gttggcctgt caatacaatg acaggcaccg acgggagctt
      721 cgcccggatg aatgcgacga catcatgtcc gttcaccttc ggcagatcaa tgtcacagat
      781 gatcgcatgg acggagaagc cggttggaga tgattggatc atggcgatcg cttgatcacc
      841 gtcaacagct tccagaatct cgtagccacc ttgtttcagt gcgaggccga ctgtttttct
      901 gacatgctcc tcgtcatcaa cgatgaggac tcgccccatt agcatgctcg tccttcccgc
      961 gccaccgcta aaaaatagtc gctcacttta tgaagtgcgc ctttgcgttg tgccagtcat
     1021 catgattccg tcgcgtactc ttcatggctg accgcaatct ccaccaacca gatatctccc
     1081 cggatacggg aaaccggtta cccatacatc ttgggacatt ttttactctt ctaatccctc
     1141 cctgctcaat cccctcgaat aggctccccc ctcgtttggg gctattcact cttctcctta
     1201 tccacagtac attgcaaccc attgaagtcc gagcctttac agagagaacc cctgctcggc
     1261 actgtcagat taatgactac gggagacggc catgcggtta acggctccgg gcaggcgaaa
     1321 cccactctcc gccggccctg aattggctca actatctcag tttctacccc cagactcatt
     1381 agggaggacc ttcgactcat gcatggaacc atcctgattg tcgatgacga tgccggcata
     1441 cgtgatacag tccgactcgt tcttgcgcgc gagggctatg gtatattaac cgccccagat
     1501 ggtgaaacgg ccatgcaatt gatggcgatg gcggacacct cggccaaagt ctgcgcgctg
     1561 ctctgtgatt tggaaatgcc acacatgggt ggcaaagcgc tcatcgaaca tttccgaaag
     1621 cactttcccg atattccgat tgtcgtgctg agcggggcat ccgatacggt atttctcgag
     1681 ggaatcgttc aggagggggt cagtgattgg ctcagaaagc ctgtcagccg tgagacctta
     1741 ttggataaag ttcgtacggc gaccaatctg ttcgcgcttc gacaacagca tcggtagaaa
     1801 cgggacgcag tattttcgat caccgcacac atggcggtgc cggttccatc gacaggaacc
     1861 atcagcaaaa ggcggatacc atcggtgctg gcaactgatt cggcccctct cttgaccttg
     1921 cttcagttcg tgactttggc gcgccgggcc ggctatgcgg agcctcgcac gtgaagatta
     1981 ggattctggg ctgccatggc tctgacggca tgctggacac cccggagggg cgccgcacct
     2041 gtaacacctg cggcttcttg ttgaatgaca gtctgcttct cgatgccggc accgttgcca
     2101 gcgcactccc cctcagcgaa caacgaaaga tccgct
//
LOCUS       DATIFR010000060         4719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_38174, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000060 DATIFR010000000
VERSION     DATIFR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38174"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VLD60_03445"
     CDS             complement(<1..453)
                     /locus_tag="VLD60_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSB44037.1"
                     /translation="MPDTSQFNTDRTYPGRWTITFSNPPINMFVPTTIVELGALMTDL
                     EGDPSVKVVVFQSANPDFFIAHLDVSKAAERPEVLGLWRDFVLRLSSTPVVSIAKIRG
                     RTRGIGNEFVLACDMRFASRQSALFGNPEIGVGLVPGGGALEWLPRLVG"
     gene            complement(484..1716)
                     /locus_tag="VLD60_03450"
     CDS             complement(484..1716)
                     /locus_tag="VLD60_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HSB44038.1"
                     /translation="MMTQTSATRPDSEQASDKTAIRPFQVSFPESELTELRRRINATK
                     WPERETVTDATQGVQLATTQALARYWGTDYDWRKCEARLNAFPQFITEIDGLDIHFIH
                     VGSRHENALPLIVTHGWPGSIIEQMKIIDPLTNPTAHGGSAADAFDVVIPSMPGYGFS
                     GRPTTTGWDPDHIARAWVTLMKRLGYKKFVAQGGDWGAVIVDLMGVQASPELLAIHTN
                     MPGIFPAEIDQAAFSGKPAPSGLSADEKVAYERLQFVYQKGIAYGFQMGLRPQTLYGI
                     ADSPVGLAAYFLDHDARSYALISRVFAGEPEGLTRDDILDNITITWLTNTALSGARLY
                     WEYWGKGGYFNVKGVSIPVAVSVFPDELYPAPRSWAEKAYPNLIHYNRLPKGGHFAAW
                     EQPKLFSEEVRAGFRSLR"
     gene            complement(1810..2334)
                     /locus_tag="VLD60_03455"
     CDS             complement(1810..2334)
                     /locus_tag="VLD60_03455"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HSB44039.1"
                     /translation="MKRTIFLSVAFATLAGVVAFTASASRHAAREAAPVFVTKLLSGY
                     RDWKLISVAHEEGNLNSFAAILGNDVAIEAYREGKLPFPDGAIIAALHWRFTQSEENN
                     KAFGRSQSFVAGAPTNVQFMVKDSKKYEATGGWGFGHFNSDEKTVDDAFMKTCFPCHQ
                     KMKERDLVFTRYAP"
     gene            2944..3342
                     /locus_tag="VLD60_03460"
     CDS             2944..3342
                     /locus_tag="VLD60_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB44040.1"
                     /translation="MKNTKLMLVLLCLMPGTLMAQQPTATVTPLMSKDLTEVPGREAV
                     MITVEYPPGSKDPIHRHNAHAFVYVLEGTIVMQVKGGKEVTLTPGQTFYEGPDDVHLV
                     GRNASSTKPAKFLVVLVKDKGAPVLVPVPE"
     gene            3439..4260
                     /locus_tag="VLD60_03465"
     CDS             3439..4260
                     /locus_tag="VLD60_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB44041.1"
                     /translation="MTNDAKSKMKIVVIGGTGLIGSKVVTKLGEHGYQTVAASPVSGV
                     NTLTGEGLAEVLKGASVVVDVSNSPSFEDTAVMEFFKTSTGNLLRYEAAAGVGHHVAL
                     SVVGTDRLPDSGYLRAKMAQETLIKKSSIPYSIVRATQFFEFLKRIADEATEGNTVRL
                     PSVRFQPLAADDVASTVAKVASGSPLNRIVELGGPEQLPFDEFIRRSLSAGKDPREVV
                     ADPHARYFGTELSERSLVPGEGAQLGKTRFADWLKSSASQPAKAKSPAATATKRA"
     gene            4298..4567
                     /locus_tag="VLD60_03470"
     CDS             4298..4567
                     /locus_tag="VLD60_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44042.1"
                     /translation="MLNLQSGDHIELFYEDAPGPTIRATVRRLLTDRDEGMGIEVEDY
                     TACWIEITVDEPSDSEMDAKQVVLLGTDFQYRLNGRRVTLRKSPD"
BASE COUNT         1034 a         1300 c         1368 g         1017 t
ORIGIN      
        1 gccgaccaag cgcgggagcc attccagcgc tccgccgccc gggaccagtc cgacgccgat
       61 ctcggggttg ccgaataggg cgctttgccg gctggcgaag cgcatgtcgc aggccagcac
      121 gaactcgtta ccgatgccgc gcgtgcggcc gcgaatcttg gcgatgctca cgactggcgt
      181 ggacgagagg cgcagcacaa agtcgcgcca aaggcccagc acctctggcc gttcagctgc
      241 cttggatacg tcgagatggg cgatgaaaaa atcgggattc gccgactgga acactacgac
      301 cttcaccgac ggatccccct cgaggtcggt cattagcgct cccaattcga cgatcgttgt
      361 gggaacaaac atgttgatcg gcgggttgct gaaggtgatc gtccaccggc ccggataggt
      421 gcggtcggtg ttgaactgcg aagtgtcagg catggcgcat ctcctcctct cttgtgaaca
      481 accctaccgc agcgacctga agcccgcgcg aacctcttct gaaaagagct tcggctgttc
      541 ccaggccgca aagtgcccac cttttgggag tcggttgtaa tggatgagat tgggatatgc
      601 tttctcggcc cagctccgtg gcgcaggata cagttcgtca gggaagacgc tcacagccac
      661 tgggatggag acgcccttca cattgaagta tcctcctttg ccccaatatt cccaatagag
      721 acgggcgccc gaaagcgccg tgttcgtcaa ccatgtgatt gtgatgttgt cgagaatgtc
      781 gtcgcgagtg aggccttcgg gttctcccgc aaagacgcgc gagatcagcg cgtagctgcg
      841 cgcgtcgtga tcaaggaaat aagccgcaag gccgaccggt gagtcggcga ttccatacaa
      901 tgtctgcggc cgcagaccca tctggaaccc gtaggcgatt cctttttgat atacgaactg
      961 tagacgctcg taagcgacct tctcgtcggc tgaaagaccg gatggcgccg gcttcccgga
     1021 aaaagccgcc tggtcaatct cggctggaaa gatgccaggc atgttggtgt gaatggcgag
     1081 caattccgga gacgcttgca cacccatcag atctacgatg accgcgcccc agtcgccgcc
     1141 ttgcgccaca aatttcttgt atccaagacg cttcatcagc gtgacccagg cgcgcgcgat
     1201 gtggtcggga tcccagccgg ttgtagtcgg cctgcccgaa aaaccgtagc ccggcatcga
     1261 tggaatcacc acgtcgaatg cgtccgctgc gcttccgcca tgggccgtgg gattggtgag
     1321 cggatcgata atcttcatct gttcgatgat cgaacccggc cacccgtgcg taacgatgag
     1381 cggcaacgcg ttctcgtgtc tcgaaccaac gtgaatgaaa tgaatatcca gcccatcgat
     1441 ctcggtgatg aattgcggga aggcgttcag tcttgcctcg cacttgcgcc aatcgtagtc
     1501 cgtgccccaa tagcgtgcca gcgcttgagt cgtcgcgagt tgcacgcctt gggttgcgtc
     1561 ggtgaccgtc tctcgttcag gccacttggt cgcgttgatg cgcctgcgca attcggtcag
     1621 ttcgctttcg ggaaaactta cctgaaacgg acgaatagcg gtcttgtcag atgcctgctc
     1681 gctgtccggt cgtgtagcac tggtttgcgt catcatctcc tccttcttac ttatagctct
     1741 cgattttgaa tcagcgattg cctcgcccga tatcgggcct tcagcttcgc ccccgctttc
     1801 ggtcattcat caaggtgcgt agcgcgtaaa gaccaggtcg cgctctttca tcttctggtg
     1861 gcaggggaaa caggttttca taaatgcgtc gtcaactgtc ttctcgtcgc tgttgaaatg
     1921 accgaacccc cagccaccgg tcgcctcata cttttttgag tccttgacca taaactgaac
     1981 gttcgtcggg gccccggcca cgaaagattg ggaacgacca aaggctttgt tgttttcctc
     2041 cgactgtgtg aaacgccagt gcaaagcggc aataattgcg ccgtccggga acggaagctt
     2101 tccttcccgg taggcctcga tcgctacatc gttgcccaga atggcagcga aactgttgag
     2161 gttaccttct tcgtgggcca cagagatcaa cttccaatca cggtatccgg acagaagttt
     2221 ggttacgaag acgggggcgg cctctcgagc ggcgtgtcga gatgcggacg ccgtgaaggc
     2281 gacaacgccg gccagagtag caaatgcaac cgataagaaa atggtccgct tcatggtgct
     2341 tcctccttcg agcggattag ttcggattca ttgaacaagt tgcaagtaca ccacgagcga
     2401 gactgcgaat ctattgcacc aaggtatcga tcgatacctt ggtatcggtc cgagtgctga
     2461 gcgtcggtcg gcgagggagc aggaggtccg tgcctcgatt tgagatgaac agacgacggc
     2521 ttacggtgtg acgtattcaa ctagtcccgg accggagtca caagttggag acagaattgt
     2581 tcaaagtaat gctcgtttct cgatattcct tcaagaacct tacgatgctt gcttcagccg
     2641 ctattgtcta tcgtactttg gcatcggtct aacgatctcc ttgtcttccg tacggtcgaa
     2701 catcgatcgt cgctccgact gataccaagg tattgcccga taccaacgtc caatatcttc
     2761 accattctgt ctgtggttta ctcgcgtgtg tgacggaaag ccatgtaaga aatggccact
     2821 gtctgcggat gcattttaga cgtcgcgcca gtcagctggg tgtcggcaag caatcgccgt
     2881 tgcgtcccga attccaaggt cgctgggcca tatggggctt ggcgaccgaa cgagaggtta
     2941 atcatgaaga acacgaaatt aatgttggtt ctgctatgtc tcatgccggg cacgctgatg
     3001 gctcaacagc ccacggccac ggttacaccg ctcatgtcga aggacctgac ggaggttccc
     3061 ggcagagaag ccgtgatgat cacagtggag tatccgccgg ggagcaagga tcccatccac
     3121 cgacacaatg cacatgcgtt cgtttatgtg ctggagggca caatagtgat gcaggtgaaa
     3181 ggtggaaagg aagtgacgct gacaccgggc cagacctttt atgaaggccc cgacgatgtt
     3241 catctcgtag ggcgaaacgc aagcagtacc aagccggcaa agttcctggt ggtcctggtc
     3301 aaggacaagg gcgctccggt gctagtgcct gtgccggaat gaatcagcaa aaaccgcaac
     3361 acaactccga gtatgtctcg gagatcaccc gtccgaactt cggaccgaca aacgaaaggc
     3421 cacacatgga ggtgaataat gactaatgat gcgaagtcga agatgaagat tgtggttatt
     3481 ggcggcactg ggctcatcgg gtcaaaggtc gtaacgaagc ttggtgagca cggctaccag
     3541 actgtggcgg cgtcaccagt ctcgggggta aataccctca cgggtgaggg acttgccgaa
     3601 gttctcaagg gcgcttcggt agtcgtcgac gtgtcgaatt cgccatcgtt cgaggacacg
     3661 gcggtgatgg agttcttcaa gacgtcaacc ggaaacttgc tcaggtacga agcagccgca
     3721 ggcgtgggcc atcacgttgc attgtcggtc gtcggcactg accgtctgcc cgacagcggc
     3781 tatctgcgcg cgaagatggc tcaggagacg ctgatcaaga aatcctcgat cccttactcg
     3841 atcgtacgtg ccacgcagtt tttcgagttc ctgaagcgca tcgccgacga ggccaccgaa
     3901 ggcaacacgg ttcgcttgcc atcagtccgg ttccagcccc tggcggcgga cgatgtcgcc
     3961 agtacggtgg ccaaagtcgc gtctggatcg ccgttgaatc gaatcgttga actgggcggg
     4021 ccggagcagt tgcctttcga cgagtttatt cgccgcagtc tcagcgccgg caaggatccg
     4081 cgtgaggttg ttgctgaccc gcacgcgcgc tacttcggca ctgagctgag cgagcgctca
     4141 ctcgttccgg gcgaaggtgc gcaactcggt aagactcgtt ttgccgactg gctcaagagc
     4201 tccgcgtctc aacccgcgaa ggcaaagtca ccggccgcaa ccgcgacgaa acgagcctga
     4261 gaatagggca gataacgaat tcggatgtgg aggtggaatg ctaaatctcc aatctggtga
     4321 tcacattgaa ctgttctacg aagacgcccc tggaccgacg attcgcgcaa cagtccgccg
     4381 tttgctgact gatcgggacg aaggaatggg catcgaggtt gaagactata ctgcctgttg
     4441 gatcgagatc actgtggatg agccgagcga cagcgagatg gacgcaaaac aggttgtgtt
     4501 gctcggcacc gacttccagt atcggctaaa cggccgccgg gtaactcttc gtaagagccc
     4561 tgactagctg atgctgttaa acctggaagt tacaagagcg gcacaagaag aaaggagagt
     4621 ttcatgagta ctatcacaac gaaagatgga acacagattt actacaagga ctggggtact
     4681 ggccccgtca ttacgttctc gcacggctgg cccttgaat
//
LOCUS       DATIFR010000061        22016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2721531, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000061 DATIFR010000000
VERSION     DATIFR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22016
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721531"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VLD60_03475"
     CDS             <1..1158
                     /locus_tag="VLD60_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB44043.1"
                     /translation="IDFLEFYAAEWLRLALPQRLGHEPGELNHRLFHPRGVTAVISPW
                     NFPLAIPTGMVSAALVTGNPVIFKPSERSSLIGHLLVGILLEAGVPEAVLACLPGGPE
                     IGQALVRHPEVVTIAFTGSKSVGLGIMKEASAVASGQRMVKRVLAEMGGKNAIIVDET
                     ADLDDAIAGVVASSTGYAGQKCSACSRAIVHASIYDLFLARLKDAMLSLRVGNPEDPG
                     TQVGPVIDERAQAKILEYIKLGNQEARMLVEGRVTQPGWYIGPTVFADVKPTDRIAQE
                     EIFGPVLAVIKAATFGEALTIANATDYALTGGVYSRSPAHLELARQGFDVGNLYLNRP
                     ITGALVHRQPFGGHRLSGIGAKAGGEEYLTQFLVTRIVSEQTLRRGFAPVE"
     gene            complement(1051..1944)
                     /locus_tag="VLD60_03480"
     CDS             complement(1051..1944)
                     /locus_tag="VLD60_03480"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44044.1"
                     /translation="MLPTGLRIRFVLCWIGLWALGVTLTPASLPAEETGIITQSADYF
                     PDALGSHWEYRGQITEGPLQTIEHKFFTNVSSVTGTRIIRGMTVTVFHDTNPGNHGPS
                     DSFYRRDVVGIVYHGSEPGTPLEKQLIPYQIVRFPMKVLSTIQQFDKTNLDFGTDMDR
                     DGVNEKADVQGTSTVVGIESITVPAGTFKDTVKVEARMGMKIHLSGAGKVASGTDIMT
                     AWFAKGVGLVKYVERQELSPLEDRGVITEIMEELESYEIKPAKASLDRGESSTEGLFT
                     DNPRDQELRQILLTSRLRSDS"
     gene            2156..2860
                     /locus_tag="VLD60_03485"
     CDS             2156..2860
                     /locus_tag="VLD60_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB44045.1"
                     /translation="MVTGASGGIGRAVCLAFAKAGWSIGVHYFQNKIAAEETIRQIEE
                     TGSASALFQADIRDQQAVRQMIESYSRQAAAPFTFVCNAGIGTSRLVLRQDEADWANV
                     IATNLTGTFHCLQAMAPPLLAQGGGSIIVIGSHAGFHGLIGQAAYAASKAGLIGLVKT
                     AAREWGPQNLRVNLVLPGWQKTGLSEGTMPETDGWSDHALWRPASIDEVASTVLHLAQ
                     LKDVSGQVWNCDSRNL"
     gene            2860..3618
                     /gene="bioD"
                     /locus_tag="VLD60_03490"
     CDS             2860..3618
                     /gene="bioD"
                     /locus_tag="VLD60_03490"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HSB44046.1"
                     /translation="MKQRRLARNAGVFITGTDTGVGKTLVTAVLAMALKKGGLDVGIM
                     KPIETGVSHTRLAQSDAARLQAIVESDDTLGAICPYQFGLPVAPLAAAQAERRAINPD
                     IIRQVYQLLSRRYACMVVEGIGGVHVPVTPKANVMDVIARLKLPVVIVGRAGLGGINH
                     ALLTIEALRRKKIPIVALVLNRTQPTRSVLARTQERTTVEILRKQSGVRVLGPLPYVS
                     GLARRFRQSVVRLARTAAIKNLEKLVKTSGRGSR"
     gene            complement(3590..4228)
                     /gene="thiE"
                     /locus_tag="VLD60_03495"
     CDS             complement(3590..4228)
                     /gene="thiE"
                     /locus_tag="VLD60_03495"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSB44047.1"
                     /translation="MPSHLARQPISGLYVILDPSVCSERPLRDALVLSAKAGAKIFQY
                     RNKTASMKEAYAEALPLRQAAADLGVLFIVNDRCDLALAVDADGVHLGQEDLPYAEAR
                     KVMGPNKIIGLSTHNADQVREADLLKPDYIGFGPIFKPGSKQDHDPIMGVAGLRAIRS
                     LTPLPIFAIGGITLDQAGEVMRAGANGIAVISAVLKAPDIKQAVSDFLSRMS"
     gene            complement(4382..5722)
                     /gene="accC"
                     /locus_tag="VLD60_03500"
     CDS             complement(4382..5722)
                     /gene="accC"
                     /locus_tag="VLD60_03500"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSB44048.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADANSLHARA
                     ADERICVGPPEAALSYRNIPNVLSAAEITGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKAKAREIVMRRGLPVTPGSPGELKSEQEAREAATKIGYPVIIKAA
                     AGGGGRGMRVVNTAEELGRAFQAAQAEAKSTFGNDSVYLERYFLEPRHIEVQILADHR
                     GHVVHLGERDCSIQRRHQKLVEETPSPAVDEKLRREIGRVAVDAVKAVHYRNVGTVEF
                     LLDKDRNFFFMEVNTRIQVEHPITEMVTGIDLIKEQIRLADGQPLSFRQQDIQLNGHS
                     LECRINAEDPEKFTPSPGLITKYSAPGGFGVRVDSVMESGAVVVPYYDSMIAKLITHG
                     RDRQESMARMRRALDEFVIEGIKTTIPLHKRILNDPDFQKGHVSTTFLDRFLTS"
     gene            complement(5757..6329)
                     /gene="accB"
                     /locus_tag="VLD60_03505"
     CDS             complement(5757..6329)
                     /gene="accB"
                     /locus_tag="VLD60_03505"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HSB44049.1"
                     /translation="MSSKKSRRSKARGTRIVLPQPFSDSRGTQDPAILATIQNRQIQD
                     MIDLLKRNQLTELEIEHAGVRIRVRYEVGGRTFAATVAEQMTAPTAATSQAAAQVSMP
                     AEDTTGLITITSPIVGTFYRSPSPDADPYVEEGDYVKKGQVLCIVEAMKLMNEIESEV
                     DGRVTKILVESTKPVEYGQALFLIDPKATP"
     gene            complement(6326..6886)
                     /gene="efp"
                     /locus_tag="VLD60_03510"
     CDS             complement(6326..6886)
                     /gene="efp"
                     /locus_tag="VLD60_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSB44050.1"
                     /translation="MISTTDFRGGVRLMVDGEPYYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGERFEEPDLEEREMQFLYATGSEYTFMDTETYEQLTFDKKQLGDNA
                     DLLKENMIAKILIYEHRPIDVELPIFIELKVVDADPGVRGDTASGGTKPAVVETGATI
                     KVPLYLEVGTVIKIDTRTRAYVERVR"
     gene            complement(6937..7431)
                     /gene="aroQ"
                     /locus_tag="VLD60_03515"
     CDS             complement(6937..7431)
                     /gene="aroQ"
                     /locus_tag="VLD60_03515"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSB44051.1"
                     /translation="MLRILVLHGPNLNLLGTREQSIYGTTSLDTLDAALTKLAEELAV
                     AVDIRQSNSEGELVTWIQEAGSGFDGIIINPAAYTHTSVAIRDAIAAVGLPAVEVHLS
                     NVHQREPFRHHSYIAGVALGQVSGLGPTGYLLALRGLHEHLTGMRKSKKALPAPRRQK
                     ASSR"
     gene            complement(7548..8372)
                     /locus_tag="VLD60_03520"
     CDS             complement(7548..8372)
                     /locus_tag="VLD60_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44052.1"
                     /translation="MASKQKSAASAMEIDRLATQLAKDPHSKAFLPLAEEYCKVGMWE
                     EAVAVLEDGLKYYPGFITAMVALGRAYDQLNQPTKARAVLEGAVKLSPDNLRAHRTLI
                     KIYAAQGLADAAIQSCNVILAMNPRDEEALSLQASLGVPAKSEPVQPEPPVPIQAKSL
                     TRDQPALSEESVEQVDATGEEPPAFRIEAPSTLGKGAAATDSPVGITTSFMDSALHEN
                     IHEQEEQSSKFEPSNGGSRSTSEPSPHAKTIAQLESWLRSIERQRRDRATSGDPSS"
     gene            complement(8376..9635)
                     /gene="coaBC"
                     /locus_tag="VLD60_03525"
     CDS             complement(8376..9635)
                     /gene="coaBC"
                     /locus_tag="VLD60_03525"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSB44053.1"
                     /translation="MDASTKGPTLVGIRIVLGVTGSIAAYKAVSLLRAFVREGAAVDV
                     AMTQSAIKFVTPLTFEVLSGRPVSTDLFEAHQEMRHLTVPEQADAIVIAPATANFLAK
                     AALGLGDDLLSTILLTAQCPLICAPAMDGGMWTHPTVVEHVRTLRARGVVVVDPEDGP
                     LASGRTGQGRLAEEERILDALQTVLAKKRDWQGYRILVSAGPTQEPIDPVRFISNRST
                     GKMGYAIAEAAQARGAQVVLVSGPTNIPPPKGVEVVPVETAEEMKKALCTRLSWATVV
                     VMAAAVADFRPKHTATQKMKKQAKTETTLDLERTTDILAELSAQRTNQILVGFAAETN
                     DLLTHATEKLNRKGLDLVVANDVTIPGGGFGSEQNAATLIERNGQTTELSLRPKRELA
                     DNILDAALRLLRTAPLHRTVSKTAEGR"
     gene            complement(9639..9995)
                     /locus_tag="VLD60_03530"
     CDS             complement(9639..9995)
                     /locus_tag="VLD60_03530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSB44054.1"
                     /translation="MIDMLSLLPQYTPDQFDSRHRLVIVAAQRAKHILQGSRHGASRY
                     TKETTIALDEVLRGEIKYLIGKEARDAMREAKRGKEGETERMAMMTGEDAREIKKELS
                     VYVDDSPKPAEAETEE"
     gene            complement(10017..10724)
                     /gene="gmk"
                     /locus_tag="VLD60_03535"
     CDS             complement(10017..10724)
                     /gene="gmk"
                     /locus_tag="VLD60_03535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSB44055.1"
                     /translation="MSTTTASASTTPSGPARSSTERRGILFIISAPSGTGKTTLCKQL
                     STNIPGLWHSISYTTRKPRPSEEHGREYFFTDEATFQSMIDRHEFVEWARVYGHLYGT
                     PRKMLADKIEQGIDVLLEIDVQGAMQVKKKFDDAVSIFILPPSMTVLRQRLQARASDS
                     TEEIQRRLQKVKEEVWSYREYAYIVRNDDLTRSLRDLESIFLAERLKTKRLDMTWIEN
                     NFILDDDSKPGDRKDLP"
     gene            complement(10721..11599)
                     /locus_tag="VLD60_03540"
     CDS             complement(10721..11599)
                     /locus_tag="VLD60_03540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSB44056.1"
                     /translation="MIRSMTGFGRRQTPWQDGSVTVELRSVNHRFLEIACRLPRSLTQ
                     LEESFKKAVQQRCTRGRVDMTVIVQGGKGRVGTVNLDQALAKQYHQALRILKKTLKLS
                     GSIDLALIAGLRDVVSVSDQPAEDPKLTKLVQKLALQALDDLEGMRKREGAALAKDMQ
                     SRIQAIRERRSSIAARSPKSVQESFERMKSRVEKLLGTEPPDLPRLHQELALYADRGD
                     ITEEIVRLDSHMIQFEQALNRAESVGKTLDFLLQEMGREVNTIGSKANDAEIAGQVVQ
                     MKAELERIREQVQNVE"
     gene            complement(11596..12258)
                     /gene="nth"
                     /locus_tag="VLD60_03545"
     CDS             complement(11596..12258)
                     /gene="nth"
                     /locus_tag="VLD60_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSB44057.1"
                     /translation="MTRTAPSPAAQRTRVQKIADALQQTMPAPKMELDHRSPWELLVA
                     TILSAQCTDERVNKVTPALFKRYRTAADLAKARLTDVEQLIRSTGFFKSKAKNIVTCS
                     QALTERFHGQVPSTMDDLTSLPGVGRKTANVVLGNAFDQPAIIVDTHVKRVANRLGLT
                     KSDDPNQIETDLQHLFPPSQWTAASQRLLLHGRYLCTARAPQCHQCPISAHCPWNKKG
                     SR"
     gene            complement(12291..13901)
                     /gene="hflX"
                     /locus_tag="VLD60_03550"
     CDS             complement(12291..13901)
                     /gene="hflX"
                     /locus_tag="VLD60_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSB44058.1"
                     /translation="MPHDKVLSPELARTICQLSQDFRRPIGVLLTRRGQVQEIIVGTD
                     LTLSPTTLAKFRAGSRSLRGLRLIRTQLHDQPLSQESLTDLAYLRLDLIGVLSVTPDG
                     HPGNLYLAHLLPPNGTGQLCNLLKSTPFHQFTMAFDQFIEELEAELQRARETHSVDRG
                     EESAILVSAASRSRAEQEDQLVELAELATSAGVTVIDRLVQRTQDGYQRYLLGSGKLK
                     DVLIQTLHKGADMVIFDQTLTPAQSRAISEMTDIKIIDRTQLILDIFARRAHSREGKV
                     QVELAQLHYLLPRLSGKGTQLSRLGGGIGTRGPGETKLETDRRRIRDRITHLERELNQ
                     FARHQDQRRARRTRYGLPVISLVGYTNAGKSTLLNVLTKSRVSAEDRVFETLDTTSRR
                     LRFPQDREVIITDTVGFIRDLPQELVGAFRTTLEELREADLLVHVVDAGARDIDAHIK
                     AVDTVLTELGFQHIPKVLVFNKCDRIPQGQAELLCHRYHAIGISALQPETLHPLLRHL
                     EQRLATILPAQPPTLVPHAAAPALASRP"
     gene            complement(14012..15073)
                     /gene="tsaD"
                     /locus_tag="VLD60_03555"
     CDS             complement(14012..15073)
                     /gene="tsaD"
                     /locus_tag="VLD60_03555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSB44059.1"
                     /translation="MKTQAVSYNGSWTEGPLLALETSCDETAAAVLAADGSVLSNVIS
                     SQHAVHQRFGGVVPELASRAHIEMIERVATSAIDEAQLTWKDLAGIAVTQGPGLAGAL
                     IVGISYAKTLAYSLDLPLVGISHLEGHIASAWIQNPQFPLPCIVLVVSGGHTHLYRKN
                     HDGTCELLGCTRDDAAGEAFDKGAQMLGLGFPGGPALDRLAQTGNPCAIRFPKSSLKR
                     GSLEFSFSGLKTALLYKLQAMNQESVEAKKRDLAAGYQEAIVTVLVEKAFTAVRQTGV
                     RALAVVGGVSANSRLRILLQQRADMEGIALSIPPLHYCTDNAAMIAAAGRQALRNGRR
                     MFIDAEALATLEPVTLGTM"
     gene            complement(15196..17622)
                     /locus_tag="VLD60_03560"
     CDS             complement(15196..17622)
                     /locus_tag="VLD60_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HSB44060.1"
                     /translation="MFERFTDKGRKIIILAREEAERHQNDYLGTEHLVLAILRESDGI
                     ALMILKKMGLSTEQIRLEIERNLPGGGTTMTFGEIPFSPRVKKVIEYGVEEARLLGHN
                     HIGSEHLLLGLLREEEGIGGKILRSLGANLLTARQLTVTFLRKSAPRERDRKSNTPAL
                     DEFGRDLTQMAQEGQLDPVIGRADEIERVLQILSRRTKNNPVLIGESGVGKTAIVEGL
                     AQRIVQSEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFID
                     ELHTLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPI
                     YVQPPSLDETVRIIQGLRDRYEEHHGVEITEDAILEAVKLSDRYITDRFLPDKAIDLI
                     DETGSRAKLQTYALPGELKAMEQELKKVSRDKELAISMQNFEEAVRHREEEERLRKLL
                     DESKREWKKNQEKHKPTIGKEDVAYVVSKMTGIPLFKLEEEESNKLLRMEDFLHKRVV
                     GQNEAISAVARAIRRSRAGLKEAKKPIGSFIFLGPTGVGKTELARTLAEFLFNTEDAL
                     IRIDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTERVRRRPYSVVLFDEIEKAHPDVF
                     NLLLQVLDDGVLTDSLGRKVDFKNTVVIMTSNIGTKLIQKGVSLGFQSTEAEQDRHKK
                     EEVMGELRRSFSPEFLNRIDEIVVFHQLDKSHLTNILDILLRELNLRLIDKGVEIEVD
                     DEVKAWLIKEGYEPLYGARPMRRTIQRAIGDPLSEEMIKGRFKDSRKIRVVLRDGAPA
                     FVEQEAMAGV"
     gene            17873..18733
                     /locus_tag="VLD60_03565"
     CDS             17873..18733
                     /locus_tag="VLD60_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSB44061.1"
                     /translation="MKSKSIGILTKPKFPEVKATLESVVTWLRARHIDVLLDTTSATL
                     LGEQGGIQKTQLAGKADVLLILGGDGTMLNAARLAGERGIPILGVNMGGLGFLTEVRL
                     EHLYPSLERVFANDFVLDERLMLGTHIHRHGETVAQGVVLNDVVVSKGTLARMIELQI
                     AIEGRFVTNLRGDGLIVSTPTGSTAYSLSAGGPIINPAVQSLILTPICPHTLTHRPLI
                     VPCDAGIDVTLTSKDEGAMATLDGQVGVALTQGDTVALKVAEHRTRLIRFPESNYYEV
                     LREKLKWGDG"
     gene            complement(18748..20100)
                     /locus_tag="VLD60_03570"
     CDS             complement(18748..20100)
                     /locus_tag="VLD60_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB44062.1"
                     /translation="MTAAQKHVIIVAGAGPAGMAVASSLAKAGHEVIVLNRDIKFGGL
                     AEYGIFPSKLKLRGGLRKQYWELLEQPNVHYFGNVSIGNGKDLSVDEVRGLGASAVVF
                     SIGAQGTKAIGVEGDSAKGVFHAKDVVYHFNRLPGFGDRHFDMGKHVAVIGAGDVMVD
                     ISHWLIRYKKVERVTAIVRRGPVERKYNPKEIRAVCANMDVEGIKNEIERIKDRMVAV
                     GQKPDDVLKGLLDEFTKCEPKVSDTKMGFKFLASPKRILVDGNNRVRGLEMEDNKLDP
                     KGEDTVAVGLKQYYEFPCDAVVFAVGDKVDETVGLPYKNGMFVTNPNKTGNDPDDALF
                     QAYDEVTGKVVDGVFLAGWARKASEGLVGVAKRDGDWCAEVVTRYLANKSSRSDTKVV
                     LDKLHALLKSRKSHPVDIKGLRALEAAEKAHRGASDSIGEFKYASNQEMLALIERGKA
                     "
     gene            complement(20212..21651)
                     /locus_tag="VLD60_03575"
     CDS             complement(20212..21651)
                     /locus_tag="VLD60_03575"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSB44063.1"
                     /translation="MKNDQNRKSSEATRIERDTMGELAVPSEAYYGVQTARAIENFPI
                     SPLRMPRSVIRAMGLIKRAAASVNHSLGLLDNKPAEAIRKAATEVVEGKLDAEFPVDI
                     FQTGSGTSTNMNTNEVISNRATELLGGTRGSKLVHPNDHVNLGQSSNDVIPTAIHIAA
                     SETIHRHLTPALTRLHKALDRKAKEFDKIVKIGRTHLQDATPVRLGQEFGGYARQIEL
                     GLARMKRAQEALSEVALGGTAVGTGLNCHPDFSAKVMAIISKETGCSFKEAQNHFEAQ
                     STQDSLVEASGELRTLAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSIMPGKVN
                     PVIAESVTMVCAQVIGNDVTVTVGGQAANFELIVMLPVMAYNLLQSIELLATASNNFA
                     AKCIEGIKANEERCKSLIEESLAMCTALAPEIGYEAAAKLAKDAYKSGKTVREVAMEQ
                     KVLPEKRLTELLDPWRMTEPGGPVGSAGG"
     gene            21736..21975
                     /locus_tag="VLD60_03580"
     CDS             21736..21975
                     /locus_tag="VLD60_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44064.1"
                     /translation="MDLPRVRVESYAGYKGEETPRAFTIDGVHFLVLEVVERWCSETH
                     GYFRVRANDQHRYVLRCHHNSGDWELVMQERSPGK"
BASE COUNT         4403 a         6109 c         6291 g         5213 t
ORIGIN      
        1 atcgattttc tggagttcta cgccgcagaa tggctcaggt tagcacttcc tcagaggctg
       61 gggcatgaac cgggcgaatt gaaccatcgg ctatttcacc cgcgtggggt gaccgcggtg
      121 atctcacctt ggaactttcc actcgcgatt ccaaccggga tggtttccgc cgcgcttgtg
      181 accggcaatc ctgtgatttt caaaccctcc gagcgatcct ccttaattgg ccatctcctt
      241 gttgggatat tactcgaagc tggtgtgcct gaagcagtcc ttgcctgttt gcctggtggg
      301 cctgagattg ggcaggcgtt ggtccgtcat cccgaggtag tgacgattgc ttttaccggc
      361 tcgaaatcgg ttgggctagg catcatgaag gaggcatcgg ccgtggcttc aggacaacgg
      421 atggtgaagc gtgtgcttgc cgaaatggga ggcaagaatg ccatcatcgt tgacgaaaca
      481 gccgatctcg atgatgcaat cgccggcgtg gtggcatcca gtaccggtta tgcagggcaa
      541 aaatgctctg cctgctcgcg ggcgatcgtg catgcctcga tatacgacct gtttcttgcg
      601 cggctcaaag acgcgatgct gagccttcgg gtgggaaatc cggaagatcc cgggacacag
      661 gttggtcctg tgatcgatga gcgggcccaa gccaagattc tcgagtacat caaactgggg
      721 aatcaggagg cgcggatgct cgtagaagga cgcgtcacac aaccaggctg gtacatcggc
      781 cccacggtgt ttgccgatgt gaagccgacg gaccggatcg cgcaagagga aatcttcggc
      841 ccggtattgg cggtcatcaa agcggcaacg ttcggggaag ccttgacgat agcgaatgca
      901 accgactatg ccttgaccgg tggagtgtac tcacgcagcc cggctcacct cgaactggcg
      961 cggcagggct ttgatgtcgg gaatctctac ctgaatcggc ccatcaccgg cgcgctcgta
     1021 catcgccagc cattcggagg gcatcggctc tcaggaatcg gagcgaaggc gggaggtgag
     1081 gagtatctga cgcaattcct ggtcacgagg attgtcagtg aacaaaccct ccgtcgagga
     1141 ttcgccccgg tcgagtgatg ctttcgccgg cttgatctcg taggattcga gctcctccat
     1201 aatctcggtg attacgccgc gatcttccaa tggggagagt tcctgtcgtt cgacgtattt
     1261 caccaggccg acaccctttg caaaccaggc ggtcataatg tctgtgccgc tggcgacttt
     1321 gccagccccg gagagatgaa ttttcattcc cattcgggct tcgactttga ccgtgtcctt
     1381 gaaagtaccg gccggtacag tgatcgactc gatgcccacc acggtcgagg tgccctgaac
     1441 atccgctttt tcattgacgc cgtcacgatc catatcggtc ccaaaatcca gatttgtttt
     1501 gtcgaactgc tgaatcgtgg acaggacctt catcggaaac cggacgattt gatacgggat
     1561 caattgcttt tccaatggcg tcccgggttc cgacccgtga tagacgatcc cgacaacatc
     1621 gcggcggtaa aaactgtccg aaggaccgtg attgccagga ttcgtatcgt gaaacacggt
     1681 gaccgtcatg cctcggatga ttcgtgtgcc cgtcaccgag gagacgttcg tgaaaaactt
     1741 gtgctcgatt gtctgcagag gcccttcggt aatttggccg cgatattccc aatggctgcc
     1801 gagcgcatcc ggaaaatagt cggccgattg tgtgatgatt cctgtctctt ccgcaggaag
     1861 cgacgcaggc gtcagagtga cgccgagggc ccagaggccg atccaacaaa gaacaaagcg
     1921 aattcgcagt ccggttggca acatgcagcg agtcctccag aattgggtct gccgctgacg
     1981 gtatcatagg catcctgaag gcagcaagca tgcggtagcg cagtcctcaa agatggaacg
     2041 cgggcttgcc ttcgtcctat cgccgaccca taatcccatg cctatggccc aacgtgattc
     2101 ctcctccagc gctgccgcat cgcatcaggc aacgccacct ccgtcaagag ctgtcttggt
     2161 taccggtgcc tcaggcggca ttggccgggc cgtgtgtctc gcctttgcca aggccggatg
     2221 gtcgatcggg gtccattatt ttcagaacaa gatcgccgcc gaggaaacca tcaggcagat
     2281 tgaggagact ggttcggcca gtgcgctctt ccaagccgat atccgagacc aacaggcagt
     2341 ccgacagatg atcgaatcct attctcgaca agcggccgcg ccattcacat tcgtgtgcaa
     2401 cgcagggatc ggcacaagcc gactcgtcct gcgacaggat gaggcagact gggccaatgt
     2461 gatcgctacg aatctgacgg gaacctttca ctgcctgcaa gccatggccc ctcctctcct
     2521 ggctcagggc ggcggctcga ttatcgtaat cggatcgcat gccggctttc atgggttgat
     2581 cggccaagca gcctatgccg cgtcgaaagc cgggttgatc ggtttggtaa aaacggcggc
     2641 gagagagtgg ggcccccaga acctgcgcgt gaatctggtg ttgcctggct ggcagaaaac
     2701 gggcctctcg gaggggacca tgccggaaac ggatgggtgg agcgaccatg cactatggcg
     2761 tccggcgtca atcgatgaag ttgcgagcac cgtgttgcat ttagctcaat tgaaggatgt
     2821 ctccgggcag gtctggaact gcgacagcag gaatctttga tgaagcaacg tcgattagca
     2881 agaaacgccg gggtttttat cactggaacc gacacgggtg tcggcaagac actggtcacg
     2941 gctgtgctag cgatggcatt gaagaagggc ggtctcgatg tcggcattat gaaaccgata
     3001 gaaactgggg tctcgcacac gagactggct cagtccgatg ccgcacgact ccaggccatt
     3061 gttgaaagcg acgataccct tggagccatc tgcccttacc agtttggatt gcctgttgct
     3121 ccgcttgcgg cggcgcaggc ggaacggcgc gccatcaatc cggacataat ccgacaagtg
     3181 tatcaactcc tctcccgtcg ttatgcctgc atggttgtcg aaggaatcgg cggggttcat
     3241 gtgccggtca cgccgaaggc gaatgtcatg gatgtgatcg ctcggttgaa gctgcccgtg
     3301 gtcatcgtcg gacgggctgg gcttggcggc atcaaccacg cgctgttgac aattgaggcg
     3361 ctccgccgga aaaagatccc gattgttgca ctggtcctca atcggacgca gccgacacga
     3421 tcggttctcg cacgcaccca agagcggaca acagtcgaga ttctccgcaa gcagtcaggg
     3481 gtgcgagtgc ttggccccct cccctatgta tcgggactgg cacgacgatt tcggcaatcg
     3541 gtcgtccggt tggcgcgcac cgctgccatc aagaatctgg aaaagttggt caagacatcc
     3601 gggagaggaa gtcgctgact gcttgcttga tatccggagc tttgagaacg gctgaaatca
     3661 cggcaatgcc gttggctcct gcccgcatga cctccccggc ctgatcaagc gtgattccgc
     3721 cgatcgcaaa gatcggcaac ggtgtcagcg aacgaatcgc ccgcaggcct gcaaccccca
     3781 taatcggatc gtgatcctgt ttagagccgg gtttaaagat cggcccaaag ccgatatagt
     3841 ccggcttgag caggtcggct tccctcacct gatcggcatt gtgagttgac aggccgatta
     3901 ttttgttcgg tcccatcact ttccttgcct cggcgtacgg taagtcttct tggccgagat
     3961 gcacaccgtc agcatcgaca gccagcgcca gatcgcaacg gtcatttacg ataaacagta
     4021 ctcccagatc ggctgcagcc tgccgtaatg gcaatgcctc cgcataggcc tctttcatcg
     4081 aagcggtctt gttgcggtac tggaagattt ttgctcctgc ctttgctgat agaacgagtg
     4141 cgtcacgcag tggccgttct gaacaaacag aaggatcgag gatgacatag aggccggaaa
     4201 tgggctgccg agcaagatga gagggcatag tggcagaatt ttagcccgct ttgatcagag
     4261 gaggctaggg caaatagaga tcgagatggg aatgtgcgat ttcaaaaact gcccacctct
     4321 cacgcacgtt ttttgttcag cagaaggctg cgactcaggc gtacagtctc cccgtggatg
     4381 gctagctcgt gaggaaccga tcgaggaacg tcgtcgagac gtggcccttt tggaagtcgg
     4441 ggtcgttaag gattcgtttg tgcaggggga tggtggtctt aataccttcg atgacgaatt
     4501 cgtcgagggc gcggcgcatg cgggccatgg attcttggcg atctcgtcca tgcgtaatca
     4561 gcttggcaat catggaatca tagtatggaa ccaccaccgc gcctgattcc atgacagagt
     4621 ctacgcggac cccgaatcca cctggcgcgc tgtacttggt aatcaagccc ggtgaggggg
     4681 tgaacttctc cgggtcttcg gcattgattc ggcattccag gctgtggccg ttcaattgaa
     4741 tgtcttgttg tctgaacgag agcggctgtc catcggccag tcgaatctgc tccttgatca
     4801 aatcgatgcc cgtaaccatc tcggtgatag gatgctcgac ttggatgcga gtgttcactt
     4861 ccataaaaaa gaaattgcgg tctttgtcca acagaaactc gaccgtgccg acgttgcgat
     4921 aatgcacagc tttcacggcg tcgacggcga cgcggccgat ctcgcggcga agtttttcgt
     4981 ccacggccgg cgacggcgtc tcctcgacca acttttgatg gcgtcgttga atcgagcaat
     5041 cccgctcgcc gaggtgcacc acatggccgc ggtggtcggc aagtatctgc acctcgatat
     5101 gccgcggttc gagaaagtag cgttccagat acacgctgtc gttcccgaag gtggatttgg
     5161 cttcagcctg ggccgcttgg aacgcgcgcc cgagttcctc agcggtgttg acgacgcgca
     5221 tgcctctgcc gccgcctccc gccgctgctt tgatgatgac cggatagccg atcttggtcg
     5281 cagcctcgcg ggcttcttgc tcgctcttga gttcaccggg gcttcccggt gtcaccggca
     5341 agccacgccg catcacaatt tcgcgggcct tggccttgtc acccatcaac gcgatgttct
     5401 ctgatgtcgg tccgatgaat ttgacaccga tggattcgca gacttcggcg aagtgggcgt
     5461 tttccgaaag gaagccgtag ccgggatgga tggcatcggc gccggtaatt tcggcggcac
     5521 tcaagacgtt gggaatgttg cggtagctta aggcggcttc gggcggtccg acacagatgc
     5581 gttcgtccgc cgctcgggca tggaggctgt tcgcatcggc ttcggaatgg atggcgacgg
     5641 tcttgatgcc gagttctttg caggcccgga tgacccgtaa tgcgatctct ccacgattgg
     5701 cgaccaagac tttcttaaac acgcaatggc tccgctatcc gattgaggga ggaaaattag
     5761 ggggtggctt ttgggtcgat gagaaagagc gcctggccat attcgacggg cttggtgctt
     5821 tccaccagga tctttgtcac acgcccgtca acctccgact caatctcgtt catcaacttc
     5881 atggcttcga cgatacacaa gacctgacct tttttcacgt aatccccttc ttccacatac
     5941 gggtctgcat ccggcgaagg cgatcggtag aaggttccca caattggcga ggtgatggtg
     6001 atgaggccgg tggtgtcctc ggctggcata ctgacttggg cagccgcttg ggaagtcgcg
     6061 gccgtagggg ccgtcatttg ctcggccacg gtcgcggcga aggttcttcc cccgacttca
     6121 taacggaccc tgatccggac ccccgcatgc tcgatctcca gttccgtcag ttgattgcgc
     6181 ttgagtagat cgatcatgtc ctggatctgc ctgttttgta ttgtggccag gatagcggga
     6241 tcttgggtgc ctctggagtc ggagaatggc tgcggcagca cgatccgcgt tccgcgcgct
     6301 ttcgatctgc gggatttctt cgagctcacc ggactcgctc cacgtaggct ctggtacggg
     6361 tatcgatctt aatcacggtg ccgacttcca aatagagggg gaccttgatc gtggcgccgg
     6421 tctcgacgac ggccggtttg gtgccgccag atgccgtgtc cccgcgaacg cccggatccg
     6481 catcgacgac tttgagctcg atgaaaattg gcagttcgac gtcgatcggc cggtgctcat
     6541 agatgaggat cttcgcgatc atgttctctt ttaagagatc cgcattgtcg ccgagctgct
     6601 ttttgtcgaa ggtaagctgt tcgtaggttt cggtatccat gaaggtatac tcgctgcccg
     6661 ttgcatagag gaactgcatt tcccgttctt cgagatccgg ttcctcgaac cgctcgccgg
     6721 accggaaggt acggtcgagc acgttgccgg acaggtagct ttttaacttg gttcggacga
     6781 atgctccccc tttgccaggt ttcacgtgct gaaactcgac gatgtaatag ggttctccat
     6841 cgaccatcag ccggacgcca ccccgaaaat cagtcgttga aatcactcgg tactcccttc
     6901 accacataat tctgagttgg atgatacttg tctacactat ctggatgatg ctttctgtcg
     6961 gcggggtgcc ggcagggcct tctttgactt acgcattccc gtcaggtgtt catgtagccc
     7021 tcggagcgca agcagataac cggttggtcc gaggccggaa acttgcccaa gcgcgactcc
     7081 tgcgatgtag gaatggtgac gaaacggctc tcgctggtgg acgttcgaca gatgcacctc
     7141 gaccgcgggt aaccccaccg ccgcaatggc atctcgaatc gcgacgctcg tatgcgtgta
     7201 ggctgcaggg ttgataataa tcccgtcgaa tccgcttccg gcttcctgaa tccatgtcac
     7261 cagttcgcct tcgctgtttg actgccggat gtcgaccgcc actgccagtt cctcggccag
     7321 tttggtcagc gcagcatcga gcgtgtccag agaggtcgtg ccatagatgg actgctccct
     7381 cgtgccgagg aggttcagat tggggccatg taagaccaga attcgcagca tgtttcaatc
     7441 ggcatattcc aagaataacg gatcgttcgc ctgcgagggt ttttcgcaga gcggcattgt
     7501 aacaggccgc ccaggaccgg tcaaactatc gaaatctctt ggaaaattta cgagctgggg
     7561 tctccggatg tcgcacggtc tcgtcgctgt ctctcgatag agcgcagcca cgattccagt
     7621 tgggcgatcg tcttggcatg aggcgagggc tccgatgttg aacgtgaacc cccgttgctt
     7681 ggttcgaatt tggaactttg ttcttcctgc tcgtgaatat tctcgtggag cgcgctatcc
     7741 atgaagctgg tagttatgcc gacaggagaa tcagttgcgg cggctccttt tcccaaggtc
     7801 gaaggtgctt cgatacgaaa agccggcggt tcctctcccg ttgcatcaac ctgttcgact
     7861 gattcttcag agagcgccgg ctgatcccgt gttagcgact tcgcttgaat cgggactgga
     7921 ggctccggtt gcaccggttc tgatttggcc ggcacaccga gggaagcctg gagcgacagc
     7981 gcctcttcgt cacgcggatt catggccagg atgacattgc aggattgaat tgccgcatcg
     8041 gccaaccctt gagcagcgta aatcttaatc aaggtgcgat gggcgcgaag gttatccggg
     8101 ctcagcttca cggctccttc gagcacggcc ctcgccttgg tcggttgatt taactggtca
     8161 tacgcacgtc ccagtgccac catcgccgtg atgaatcccg gatagtattt gagtccatct
     8221 tccagaactg cgacggcctc ttcccacatg ccgaccttgc aatattcttc ggcaaggggc
     8281 aggaatgcct tggaatgagg gtctttcgcc agttgagtcg ccagccggtc tatttccatc
     8341 gcgctcgcgg cgcttttctg tttagaagcc atggcctaac gtccttccgc tgtcttcgag
     8401 acggttcggt gcaatggagc ggtgcgaagc aatcggagtg ctgcatccag aatattatcg
     8461 gccagttcac gtttgggtct gagcgagagt tccgttgtct gtccattccg ctcgatcaag
     8521 gttgccgcgt tctgttcact gccgaacccg ccgcctggta tggtcacgtc gttggcgacg
     8581 accaggtcga gaccttttct gttcagtttc tccgtggcat gggtcagtag atcgttagtt
     8641 tcggcggcaa agccgacaag aatttgattg gttcgctgag ctgaaagctc agccaagatg
     8701 tcggtggtcc gttcgagatc cagcgttgtt tccgtcttgg cctgtttctt catcttctgt
     8761 gtcgcggtgt gctttggacg gaaatctgcc acggccgccg ccatgacgac gacggtagcc
     8821 cagctcaaac gggtgcagag ggccttcttc atctcttccg cagtttctac cggcacgacc
     8881 tcgacacctt tcggaggtgg aatgttggtc ggtccgctta cgagcaccac ctgagctccc
     8941 ctcgcctgcg cggcttctgc aatggcatac cccatttttc ccgtggagcg attcgagatg
     9001 aagcgcacgg gatcgatggg ctcttgagtc ggccctgccg atacaaggat acgatagcct
     9061 tgccaatcgc gttttttggc cagcacagtc tgaagggcat cgagaatacg ttcttcctct
     9121 gcaaggcggc cttgtccggt gcggcctgaa gccagcggtc cgtcttcggg atcgaccacg
     9181 acgacgcctc tcgctcgcag ggtgcgtacg tgttctacga ccgtcgggtg ggtccacatg
     9241 cctccgtcca tggccggggc acagatcaag gggcattggg cggtcaacaa aatcgtcgag
     9301 aggagatcat cgcccagtcc taatgcagcc ttggccaaaa agttggccgt ggctggagcg
     9361 atcacgatcg cgtcagcctg ctcggggacc gtgagatgtc tcatttcctg atgggcttcg
     9421 aacaggtcgg tcgagaccgg ccgtccggag agaacctcga aggtcagcgg cgtaacgaac
     9481 ttgatggccg actgcgtcat ggcgacatca acggcggcgc cttcgcgaac gaaggcccgg
     9541 aggaggctga cggctttgta ggctgcgatg ctgccggtca cgccgagcac gatgcgtatt
     9601 ccgacgagcg tcgggccttt cgtgctggcg tccatacctt attcctcggt ttctgcctca
     9661 gccggtttgg ggctgtcatc gacatagacg ctgagttctt tcttgatctc gcgagcatct
     9721 tcgcccgtca tcatcgccat gcgctcggtt tcgccctcct tgccgcgctt ggcttctctc
     9781 atggcatccc gtgcttcttt tccgatgaga tatttgatct cgccccgaag gacttcgtcg
     9841 agggcgatcg tcgtttcttt tgtatagcgc gacgcgccat gtcgcgatcc ctgcagaatg
     9901 tgtttggcgc gttgggccgc cacgatgact aaccggtggc gcgaatcgaa ctggtccggt
     9961 gtgtactgtg gtaacaggct tagcatgtcg atcatggatt cggtactccc cttcggttat
    10021 ggtagatctt tccgatctcc gggttttgaa tcatcgtcga gaatgaaatt gttctcgatc
    10081 caggtcatgt ccaatcgctt cgttttgagg cgctcagcca gaaagatgct ctcgaggtcg
    10141 cgcaacgagc gcgtcaagtc gtcgttccgc acaatatagg catattcccg atagctccag
    10201 acctcttcct ttaccttttg caaccgccgc tggatctctt cggtcgagtc cgaggctctg
    10261 gcttggaggc gttgtcgtag cacagtcatc gacggcggaa ggataaagat cgaaaccgcg
    10321 tcgtcgaact tcttcttgac ctgcatggcg ccctggacgt cgatttccag cagcacgtcg
    10381 atcccctgtt cgattttatc ggccagcatt tttctcggtg tgccgtagag gtgcccgtag
    10441 acgcgtgccc actcgacgaa ttcgtgtcgg tcgatcatcg actgaaaggt cgcctcatcc
    10501 gtaaaaaaat attcgcgacc atgttcttcg cttggccgtg gttttctggt cgtataagag
    10561 attgagtgcc agagtcccgg gatattggtc gaaagctgtt tacaaagcgt ggttttcccc
    10621 gtcccggacg gcgccgaaat aataaaaaga atcccccgcc gctcggtcga agaccgagct
    10681 ggcccagaag gggtggtgga ggcgctggcg gttgtggtgc tcattcgacg ttctgaacct
    10741 gctccctgat ccgttccaac tcggctttca tctgtacgac ctgaccggca atctcggcgt
    10801 cattggcttt cgagccgatc gtattcacct ctcgtcccat ttcctgaagg aggaaatcga
    10861 gagttttgcc cacagattcc gcgcggttaa gcgcctgttc aaactgtatc atatgtgagt
    10921 ctaatctgac tatttcttct gtaatgtccc cacggtccgc gtacagggct aattcctgat
    10981 gaagacgagg gaggtccgga ggctctgtcc caaggagctt ctcgacacgg cttttcatcc
    11041 gctcgaaaga ctcctgaacc gactttggcg atctggcagc gatggacgat cgacgttccc
    11101 tgatagcctg aattcggctc tgcatgtcct tggccagggc ggcgccttcc cgtttcctca
    11161 tcccttccag atcatcgagc gcctgtagag ctaacttctg gaccagcttg gtcagtttcg
    11221 gatcttctgc cggttggtcg gaaaccgaga ccacatctcg aagccccgcg atgagcgcga
    11281 gatcaattga cccgctcagc ttgagggttt tctttaagat acggagggct tgatggtact
    11341 gttttgcgag cgcctggtca aggttgaccg ttcccacacg tcccttgccg ccctggacga
    11401 tgacggtcat atcgacgcgt ccccttgtgc agcgctgctg gacggctttc ttgaaactct
    11461 cctccagttg cgtcagcgac cgcggcaagc ggcaggctat ttcgagaaac cgatggttta
    11521 cggaacgcag ttcaactgtc actgagccgt cttgccaggg tgtctgtcgt cgcccgaatc
    11581 cggtcatgct acggatcatc gtgagccttt cttgttccag gggcaatgag ctgaaatcgg
    11641 gcattgatga cattgtggcg ctcgtgccgt gcagagataa cggccgtgaa ggagcaagcg
    11701 ttgcgatgcc gctgtccatt gtgatggagg gaataagtgc tgcagatccg tttcaatctg
    11761 gttcggatcg tctgatttgg ttagccccag tctgttggca acgcgcttta catgcgtatc
    11821 cacgatgatg gccggttgat cgaatgcatt cccaagcacg acgttggcag ttttcctccc
    11881 gacccccggc agtgaggtca agtcgtccat agtgctcggc acctgcccgt gaaatcgttc
    11941 cgtcaaggcc tggctgcagg tcacaatgtt cttggccttg ctcttgaaga agccggtcga
    12001 acgaatgagt tgttcgacgt ccgtgagacg ggccttcgcc agatctgcag cggttcgata
    12061 gcgtttgaaa agtgccggtg tgactttgtt gactcgttca tcggtgcact gggctgacaa
    12121 aatagtggcc accagtaatt cccaaggaga ccgatggtct agctccatct tgggtgcggg
    12181 catggtctgt tggagcgcat cggcaatttt ttgcactcgt gtgcgctgag cggctggtga
    12241 gggggctgta cgtgtcatgg atgaacttgt ggaaggcgat tgtgcagcgg ttacggccga
    12301 gatgcaagag ccggggcagc agcatgtgga acaagagttg ggggctgagc aggcaggatc
    12361 gtcgcaagcc gttgctcgag atgacggaga aggggatgga gggtctccgg ttggagcgcc
    12421 gaaatgccaa tagcatggta gcgatggcac agcagttcgg cttgtccctg aggaatgcga
    12481 tcgcacttgt tgaagaccag gactttgggg atgtgctgaa agcccaactc tgtcagtacc
    12541 gtatcgacgg ctttaatatg tgcatcgatg tctctggccc cggcatcgac cacgtgcacc
    12601 agcaggtccg cctcgcgcaa ttcctccaga gtcgttcgga acgccccgac gagttcttgc
    12661 ggaagatccc ggatgaaacc gacggtgtcc gtaatgatca cttctcgatc ctgtgggaat
    12721 cggagcctcc ggctggtcgt atcgagcgtt tcaaagactc ggtcttcagc cgagacacgg
    12781 ctcttggtaa ggacattcag gagggtcgat tttccggcat ttgtgtaccc gacgagagag
    12841 ataaccggca agccatacct tgtacggcgg gcgcggcgct ggtcctggtg acgggcaaat
    12901 tggttcagct ctcgctccaa atgtgtgatg cgatctctga tccgtcgtcg gtctgtttct
    12961 aacttcgttt cgccgggccc cctcgtcccg atgccaccgc cgagacgaga cagttgcgtg
    13021 ccttttccgg aaagtcgtgg aagcaggtaa tgtagctgag ccagctcaac ctgaaccttt
    13081 ccttcccgac tgtgggcccg gcgggcaaaa atatccaaaa tgagttgagt gcggtcgatg
    13141 atcttgatat cggtcatctc agaaatggca cgcgattggg caggagtcag tgtctgatca
    13201 aagatcacca tatcggcgcc cttgtgaagc gtctggatca ggacatcctt caatttcccg
    13261 cttccgagga gatagcgttg atagccgtcc tgagttcgtt gtaccagacg atcgatgacg
    13321 gtcactccgg ctgaggtcgc gagctccgca agctcaacca gctgatcctc ctgctcggct
    13381 cgactacgcg acgcggcgct gaccagaatt gccgattcct ctcctcggtc aactgagtga
    13441 gtctcgcgcg cccgttgcag ttccgcctcg agctcttcga tgaattgatc gaatgccatc
    13501 gtgaattggt ggaacggggt agatttgaga agattgcaga gctgaccggt tccgtttggc
    13561 ggaagaagat gcgcaaggta gagattgccc ggatgtccgt ctggagttac ggagagcacc
    13621 ccaatgaggt ccaaacggag gtatgcaagg tccgttaaag attcctgact cagcggctga
    13681 tcgtgcaatt gcgttctgat taaccgtaag ccgcgcaagg atcgggatcc agcgcggaat
    13741 ttggccagag tggttggaga caacgtgagg tctgtcccca caatgatttc ttggacctgt
    13801 ccccttctgg tcaacagaac gccgatggga cgtcggaaat cctgcgagag ctggcatatg
    13861 gttctcgcca gttcggggga gaggactttg tcgtgaggca cacggcgtcg atagaggcgt
    13921 tcaagtgctt caatctggct ggatcggagg ccggttactt gtccgtgaat gtctgaaatg
    13981 agctcgctcc ctttagattc tagattcgtc gttacatcgt gcccagagtt accggctcca
    14041 aggtagccag cgcctctgca tctatgaaca ttcgccgtcc attccgcaga gcctgccggc
    14101 ccgcggcagc aatcatggcg gcgttatcgg tgcagtaatg caacggcggt atactcaacg
    14161 caatcccttc catatccgcc cgctgttgga ggagaattct gaggcgcgaa ttggctgata
    14221 cgccgccgac gaccgctaat gctctgacgc cggtttgacg aaccgctgtg aacgctttct
    14281 cgaccaagac tgtaacgatc gcttcctggt accccgctgc aaggtcacgc tttttcgctt
    14341 cgacagattc ctgattcatc gcttgaagtt tatagagcaa tgccgtcttt aacccactga
    14401 agctgaactc gagactcccc ctcttcaacg acgactttgg gaaccggatc gcacacgggt
    14461 tgccggtttg agcgagacga tccaatgccg gaccgccggg gaagccgagc cccagcattt
    14521 gagcaccctt gtcgaaggcc tcccctgctg catcatccct tgtgcagcct aacagctcac
    14581 atgtgccgtc atgattcttc cggtagaggt gcgtatgccc gcctgacaca accagtacaa
    14641 tgcagggtag ggggaactgg gggttctgga tccaggcaga ggcgatgtgt ccttccaaat
    14701 gactgatgcc gacaagtgga agatcgagac tgtaggcgag tgttttggcg taactgatcc
    14761 ccacaatcaa cgctcctgcc aaaccaggcc cctgcgtgac ggcaattccg gccaagtctt
    14821 tccaagtcag ctgtgcttcg tctatggcgg aggtggccac ccgttcaatc atctcgatat
    14881 gtgcgcgaga ggcgagctcc ggtacgacac cgccgaaccg ttggtggacg gcatgctggg
    14941 aggagatgac gttggagagg accgatccat cggctgcgag aacagcggca gccgtttcat
    15001 cacaggacgt ttcgagagcg aggagtgggc cttcggtcca tgagccgttg taggaaacag
    15061 cctgtgtttt cataaaaaga gtcggggatg attgtccccg gaaagcagtg cgacccttgc
    15121 cgccattgta gcaacaaggg tcgctgtgat gtgatgtcgt gattggttaa gaaggacgtg
    15181 attcacagcc gtccgttaga caccagccat ggcctcctgc tcgacaaaag ccggtgcgcc
    15241 gtcacgcaac acgacacgaa tcttccggct atctttaaag cgacccttga tcatctcttc
    15301 cgagagagga tcgccgatcg cgcgctggat cgtccggcgc atgggtctgg cgccatagag
    15361 gggctcatag ccttccttga tcagccaggc tttgacctcg tcgtccacct cgatttccac
    15421 acccttgtcg atgaggcgca ggttcagctc acgcaacaga atgtcgagaa tattcgtgag
    15481 atgcgacttg tccagttggt ggaagactac gatctcatcg atccggttca aaaattccgg
    15541 actaaacgag cgtcgcagtt ctcccatgac ttcttctttc ttgtgacgat cttgctcggc
    15601 ttccgtgctc tggaacccaa gcgatacacc tttttggatc agcttcgtcc cgatattgga
    15661 tgtcatgatc acgacggtgt ttttgaaatc aaccttccgt cccaggctgt cggtcaacac
    15721 accatcgtcc aacacttgca gcaagagatt gaagacatcc ggatgggcct tttcgatttc
    15781 gtcgaagagg accaccgaat agggccgacg gcgaacccgc tcggtcaact ggccgccttc
    15841 ctcatagcca acgtaaccgg gaggtgctcc gaaaagccgc gaactggtga acttttcctg
    15901 atattcggac atgtcgatcc gaatgagcgc atcttccgta ttgaagagga actcagccaa
    15961 ggttctggcc aactcggttt tcccgacacc ggtcggtccg aggaagatga aggagccaat
    16021 cggcttcttc gcctccttga ggccggcacg cgaccgccga atagcacgag ccaccgctga
    16081 aatggcttcg ttctggccga cgacacgttt gtgcaaaaaa tcttccatgc gaagaagttt
    16141 attggactct tcttcttcga gcttgaagag cgggatgccc gtcatcttcg aaacgacata
    16201 cgccacatct tccttgccga tggtcggctt atgcttctcc tgatttttct tccactcccg
    16261 cttggattcg tcgagcaatt tgcgaagccg ttcctcttct tcgcgatggc ggaccgcttc
    16321 ttcgaaattc tgcatcgaaa tcgccagctc tttgtcgcgc gagactttct tgagctcctg
    16381 ctccattgcc tttaattcgc cgggcaatgc ataggtctgg agttttgcgc gcgaaccggt
    16441 ttcgtcgatc aaatcgattg ctttatccgg caggaagcgg tcggtgatgt accggtcgga
    16501 cagcttcacc gcttcgagga tggcatcttc ggtgatctcg acaccgtggt gttcttcata
    16561 gcgatcgcgc agcccttgaa tgatgcgaac tgtttcatcg aggctcggcg gctggacata
    16621 gatcggttgg aaacggcgct tgagcgcccc gtccttttca atatgtttac gatattcatc
    16681 caaggtggtc gcgccgatgc actgaatctc tccgcgcgat agcgcaggct tcaacatgtt
    16741 ggacgcgtcg atggagccct cggcagcgcc ggctccgacc aacgtatgca actcgtcgat
    16801 gaagatgatg atgttgccgg cttgcacgat ttccttcatc acgaccttga ggcgttcttc
    16861 gaattggccc cgatatttcg tgccggcgac caaagagccg aggtccaagg cgataacgcg
    16921 gcgtgacaac aagttgtcag gcacttcaga ctgcacgatc cgctgcgcca atccctcaac
    16981 gatggcggtt tttccgacac cggattcgcc gatcagaacc ggattgtttt ttgtccgacg
    17041 gctgagaatc tgcagaacac gttcgatttc gtcggctcgt ccaatgaccg gatcaagctg
    17101 gccttcttgc gccatttgcg tgaggtcacg tccgaactcg tcgagcgccg gcgtgttgct
    17161 cttccggtcg cgttcgcgtg gagcagactt gcgcaggaag gtcacggtca gttgtcgcgc
    17221 agtgaggaga ttcgcaccga ggctgcggag gattttccca ccaatcccct cctcctcacg
    17281 gagcaatccc aagaggagat gttcgctgcc gatgtggttg tggccgagga gacgggcttc
    17341 ttcaaccccg tactcaatga cctttttcac tcgagggctg aagggaatct ctccgaatgt
    17401 catggttgtg ccgccgccag gcaaattccg ctcgatctcc aggcggattt gctctgtgga
    17461 gagccccatc ttcttcaaaa tcatcagggc gatgccatcg gattcgcgga ggatggctaa
    17521 gacgagatgc tcggtcccga ggtagtcatt ctgatgacgc tcggcctctt ctctcgccag
    17581 gatgatgatc ttccgaccct tgtccgtgaa tcgttcgaac atcctgcctc cttattgatg
    17641 ggggtctgta cgtatcactg actgcgcaaa cccttcgaaa aatctggtct aattctaact
    17701 agaagggtgt cgagtgtcaa gattacgaaa gaatcgaacg acctcactct tcacacatag
    17761 ggaatgctgg cattcgagcg atgtccattc gttgacttca aaagaaccct cccgatagaa
    17821 tcccgcacgt tccggccaaa atcgcgtggt tgtttatgac acgatgggca cgatgaaaag
    17881 taaaagcatt ggtattctca ctaaacctaa atttcccgaa gtcaaggcta ccttggaatc
    17941 ggtagtcact tggctgaggg ctcgacacat cgatgtgttg ctggatacga cgtcagcgac
    18001 tctgctcggc gagcagggcg gaattcagaa aactcaattg gcgggaaagg ccgacgttct
    18061 gctgatcttg ggcggcgacg ggaccatgct caatgcggca cgtttggctg gagagcgagg
    18121 cattcctatt cttggcgtca atatgggtgg gctggggttt ctcaccgaag tccgattgga
    18181 acatctgtac ccgtcgctgg aacgggtgtt cgccaatgac ttcgttcttg atgagcgtct
    18241 catgcttggc acacatattc atcgccatgg tgaaacggtc gcgcagggag tggtgctcaa
    18301 cgacgttgtt gtaagcaagg gcacgctggc tcgcatgatt gaattgcaga tcgccatcga
    18361 aggacggttc gtcaccaact tgcgtggcga cgggttgatc gtgagcaccc cgacgggatc
    18421 gaccgcgtat tccttgtcag caggagggcc gatcatcaat ccggcggtcc aatcgttgat
    18481 tctgacgccg atctgtccgc atacgctcac ccatcggccg ttgatcgtac cgtgcgatgc
    18541 gggaatagac gtgacgctga cgagcaagga tgagggggcg atggccacac tcgacgggca
    18601 agtgggcgtg gcactgacgc aaggcgatac ggtcgcgctc aaagttgcgg agcatcggac
    18661 caggttgatc cgctttccgg aaagcaatta ttacgaggtt ttgcgggaaa agctgaaatg
    18721 gggcgacgga taatcagacg ctagagttta ggccttcccg cgctcgatca gcgcgagcat
    18781 ttcttggttg ctcgcgtact tgaactcccc gatagagtcc gacgcacctc tgtgagcttt
    18841 ctcggcggct tcgagagcac gcagtccctt gatatcgacg ggatgactct tgcgggattt
    18901 gaggagagca tgcagcttgt cgagcaccac cttcgtgtcg ctgcggctcg atttattggc
    18961 caagtatcgc gtcacaactt ctgcgcacca gtcaccgtcg cgtttcgcca ctccaacgag
    19021 gccttcgctg gcttttctgg cccagccggc cagaaagaca ccgtctacca ctttccctgt
    19081 gacttcgtca taggcctgga agagcgcatc atcggggtca ttgcccgtct tgttcgggtt
    19141 agtgacgaac atgccgttct tatagggtag gcctacagtc tcatcgactt tgtcgccgac
    19201 ggcgaaaacg actgcatcgc acgggaattc ataatactgc ttcaggccga ctgccaccgt
    19261 atcctcgcct ttgggatcga gcttattgtc ttccatctcg agcccacgca ccctgttgtt
    19321 gccatcaacg agaatgcgct tcggcgaagc caggaacttg aaacccatct tggtgtcgct
    19381 gaccttcggt tcgcatttcg tgaattcgtc taagagcccc tttaacacgt catcgggctt
    19441 ttggccgact gcaaccatac gatccttaat acgttcgatc tcgttcttga tgccttcgac
    19501 atccatgtta gcgcagactg cccggatttc tttggggttg tatttccgtt ccaccggtcc
    19561 gcgccggaca atcgccgtta cccgttccac cttcttatag cggatcagcc agtgggaaat
    19621 atccaccatc acatcccctg ccccaatcac cgccacgtgc tttcccatgt cgaaatgccg
    19681 gtcgccgaag ccaggcagcc gattgaagtg gtatacgaca tcctttgcat ggaagacccc
    19741 cttggcggag tctccttcaa caccgatggc tttggtccct tgagccccga ttgagaagac
    19801 gacagcgctg gcaccaagcc cccgtacctc atcgacgcta agatccttac cgttaccgat
    19861 tgaaacattg ccgaaataat gcacgttcgg ctgttcgaga agctcccaat attgtttcct
    19921 gaggccgccg cgcaatttga gcttcgaggg aaagatccca tattccgcaa gtccgccgaa
    19981 cttgatgtcc cggttcagga caataacttc atggcctgcc tttgcgagag aggaagcgac
    20041 tgccatgcca gctggtccgg caccggccac aataatcacg tgcttctgcg ctgcggtcat
    20101 gcgttgttcc ctaggtaaag tggatagaga aaagtctaga acctcaacag ttccgcggac
    20161 tttagcatag ccattcctga agtgtcaaaa tatcctgtgt ggagcgctca gctagccgcc
    20221 tgcactccct accggtccac caggctcagt catacgccat ggatcaagca gctcggtcag
    20281 gcgtttctcc ggcaggacct tttgctccat cgcgacttca cgcaccgttt tgcctgattt
    20341 gtaagcatcc ttcgcaagct tggctgccgc ttcatagccg atctcggggg ccaaggccgt
    20401 gcacatggcg agactttcct cgatcaaact cttgcaacgt tcttcattgg ccttaatgcc
    20461 ttcgatacat ttcgcggcaa agttattgga ggcggttgcg aggagttcga tggattgcag
    20521 gaggttgtag gccattaccg gcaacatgac aatcaattcg aagttagccg cctgtccacc
    20581 gacggtcacg gtgacatcgt tgccgatcac ctgcgcgcag accatcgtga ccgattcggc
    20641 aatgaccgga ttgaccttgc ccggcattat cgaggagccc ggttgtgttt ccggcagatt
    20701 gatctctcct aatccgcagc gtgggcctga accgagccag cgaatgtcgt tggcaatttt
    20761 catgaggctc actgctaacg tgcgcaactc cccgctggcc tcaaccagcg agtcctgcgt
    20821 ggactgggct tcaaaatggt tttgcgcttc cttgaacgaa caaccggtct ctttggaaat
    20881 gatcgccatc actttcgcgg aaaagtccgg atgacaattc aatccggtcc ccaccgccgt
    20941 gccgccgagc gcgacttcgc tcaaagcctc ttgtgcgcgt ttcatccggg cgagacccaa
    21001 ttcgatctgt ctggcgtacc ctccgaactc ctggccgaga cgaaccggcg tggcgtcctg
    21061 taaatgggtt cggccgatct tgacaatctt gtcgaattct ttcgctttgc gatcaagcgc
    21121 cttatggagc cgcgtgagcg cgggagtgag gtgtcggtga attgtttccg atgccgcgat
    21181 atggatggcc gttggaatga cgtcgttgct ggattggccc agattcacat ggtcgttcgg
    21241 atgcacgagt ttgctgcccc gagtgccacc aagcaattct gtggcccggt tcgatatcac
    21301 ctcgttggta ttcatgttgg tcgaggtgcc ggagccggtt tggaaaatat caaccgggaa
    21361 ctctgcatcg agttttcctt ccacgacttc ggtcgctgcc ttcctgatgg cttcggccgg
    21421 tttattgtcg agcagtccga gcgaatgatt caccgatgcg gcggcccgtt taattaagcc
    21481 catggccctg atgacggagc ggggcatgcg cagcgggctg atcgggaaat tttcgattgc
    21541 acgggctgtt tggaccccgt aataggcttc agatggaacg gctaactcgc ccatcgtatc
    21601 ccgttcgatt ctcgtcgctt cggatgattt tctgttctga tcgttcttca tgccttcgac
    21661 tccataagta agggttctca ctcaccgcgg gcgccatgat aaactcagcc tacctacttg
    21721 cacaagggtg tggagatgga cctaccgcgt gtcagggttg aatcctatgc gggctacaaa
    21781 ggggaagaga cgccgcgtgc gttcacgatc gacggtgtcc attttctcgt gctggaggtg
    21841 gtcgaacggt ggtgctcgga gacccacggt tactttcggg ttcgagcgaa cgatcagcat
    21901 cgctacgtgc tgcggtgtca tcacaatagc ggagattggg aactggtgat gcaggaacgg
    21961 tcacctggaa aatagaaagg cccgcgctcg cgggcctttc ggggtgagct gttctg
//
LOCUS       DATIFR010000062        13250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1380446, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000062 DATIFR010000000
VERSION     DATIFR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380446"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VLD60_03585"
     CDS             complement(<1..882)
                     /locus_tag="VLD60_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB44065.1"
                     /translation="MSKHIAILGAGPGGYVAAIRAAQLGARVTVVESQALGGVCLNWG
                     CIPSKALLSVVELGDKIKKAGPLGIQISGPVAYDLAQMVARKNQVVAGLVKGIATLFN
                     AWNIEHIEGTGVIEGPGTVRVSMKEGTEQVVTADAIVIATGSSWPNLPQFPIDGVQIL
                     TSQQLLDLQQVPARMLIIGGGVEGCEFACLYSGLGTDVTVVELMERILPLEDEEISST
                     MTRELKKRNVTVMTGTTVESLNRNPTTILVRCKDGTSFEVEKLLVSVGRGFNSRGIGL
                     DRAGVQTGRRGDILVNER"
     gene            complement(892..1278)
                     /gene="gcvH"
                     /locus_tag="VLD60_03590"
     CDS             complement(892..1278)
                     /gene="gcvH"
                     /locus_tag="VLD60_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSB44066.1"
                     /translation="MIPADLRYHQEHEWARVSGSQATVGISHFAQDALGDIVFIDLPK
                     IGAVVKAGQQIGEVESTKTTSSIYTPVSGTIVKVNADLKDHPEVVNSDPYGKGWMVVV
                     DLSNAGEVDKLMTAAQYEAFLSTQKH"
     gene            complement(1317..1736)
                     /gene="ndk"
                     /locus_tag="VLD60_03595"
     CDS             complement(1317..1736)
                     /gene="ndk"
                     /locus_tag="VLD60_03595"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSB44067.1"
                     /translation="MSERTLAIIKPDAVKKHAIGDIINRYEKAGLKPVAMKLMQMSKM
                     TAEGFYAVHKARPFFDSLCSFMCSGPSVVLVLQGDNAIKKNRELMGATDPAKAEAGTI
                     RKAHGANIEFNAVHGSDSPETAQFEITYFFPGMEILG"
     gene            complement(1834..2865)
                     /gene="mtnA"
                     /locus_tag="VLD60_03600"
     CDS             complement(1834..2865)
                     /gene="mtnA"
                     /locus_tag="VLD60_03600"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSB44068.1"
                     /translation="MVPTVEWKDGAVRLIDQSRLPEQVEFLDCRDVEAVVQAIRELKV
                     RGAPAIGVTAAMGVALGAQSIAATDYQEFSTALFAICDRLAAARPTAVNLFWAIERMR
                     QRVAALRTESVAAIKAALMKESQAILDEDIALCKAMGRHGAALIATGQTVLTHCNAGA
                     LATAGYGTALGVIRAAWEQGKKIQVIADETRPVLQGARLTAWELMQDKIPVTLITDNM
                     AGSLMREGKIHLCVVGADRIAANGDVANKIGTYSVAVLAKAHGIPFYVAAPYSTIDLK
                     TKTGAEIPIEQRNPLEVTTIHGSHPVAPKGVAVYNPAFDVTPAELIAGIITERGVFKP
                     HEIAEHFAG"
     gene            complement(2992..3189)
                     /locus_tag="VLD60_03605"
     CDS             complement(2992..3189)
                     /locus_tag="VLD60_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44069.1"
                     /translation="MPKLVRIVIQLPEELKTQLDTLKQQGYTTSGFIRAMLERELAKL
                     DQRAEQKPVTLLPVGKRANSR"
     gene            complement(3402..5939)
                     /locus_tag="VLD60_03610"
     CDS             complement(3402..5939)
                     /locus_tag="VLD60_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HSB44070.1"
                     /translation="MLPSLTLAFTCGLVVGSLIPYFPFSVSLLLFTIAIGLSILEVRQ
                     RVVGSHATAGFGCLLLGIIYWIVAVEGFSKVVFVESEQNLLQTVTGRIMAPVQQAPDR
                     LLMVVQPDGEMTAPGRPRLIRLTWRMPERLLFEGDRIRFKAKLRVPSGTSNPGGFDYA
                     TYLERQGIDAVATATGVDGVEFLESGQASAWWRVWNRIDRWRGSIRLAALQSLSQPAL
                     GLFLGIIVGDRGFLDAELRDQFMVTGTVHLLSISGSHLGLVALLVFVSVRAAVLRLPQ
                     AWLLAMSRTITATRIAAAATVVPVTAYACLAGAELATVRSLVMVLTTLLAKWLGYDQR
                     MFHALALAAMVILLHDPQALSDISFQLSFLSVCAIAAWLSWSLPSEEAEPSHHATALA
                     TGMKWSRDVVVMSAAVTLATTPLVAFYFNQVSWLGLVTNIVAVPLMGLLLVPLGLFAG
                     LGQIMSGGTVLPLSSLLQLLIDWFIEGIHFVSRMPGGEWHVASPSILTMLVFYACLGV
                     IWLRWRQVLFRMTAGAGIMLLLLWWIWSPRIALEGDQFLVTFLDVGQGDSTVIEWPDG
                     RVVVIDGGATYERFDMGRGVVAPYLWNRGIRTIDHMVGTHPQLDHVGGLTWLLRHFNV
                     KHYWGTGDGRDELFYRRLLQALAERGLTEQQAKEGQEILSSHDCRLMVLNPPDAAVTR
                     EQQPANHREGHLLNNRSIVTELSCGAHRMIFAADVEQDSLRRMQLTSRREPVAVLKVP
                     HHGAISSLNPEWIGGLRPQHAVFSVGRHNPYGHPAPKVVDAYAGQGASIYRTDLDGGI
                     WIAGKSSNSILQVHRTRDEKLHPTALSACLLDCEIANWRRLAGQWMQ"
     gene            complement(5997..7346)
                     /gene="glmM"
                     /locus_tag="VLD60_03615"
     CDS             complement(5997..7346)
                     /gene="glmM"
                     /locus_tag="VLD60_03615"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSB44071.1"
                     /translation="MRKLFGTDGVRGIANLEPMTSETAMQLGRAAAHIFKRRAGRHQI
                     VIGKDTRISGYMLEAALVSGICSMGVDVLLVGPMPTPAIAFLARSLRADAGVMISASH
                     NPYQDNGIKFFSNDGLKLPDEIEARIEQLIVSNEIAHLRPTADAIGKAFRIDDAEGRY
                     IEFVKRSLPKELDFQGIKLVVDCANGAAYKVAPTVLRELGAKVEVIGNKPDGMNINAG
                     CGAVHPELLQKAVREHNANLGVALDGDADRAIFVCEQGHVVDGDHMMAALGLDLHRNG
                     LLARETVVGTVMSNFGLELAMTRAGIQLVRTAVGDRYLLERMMADGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQVLSLMKRTEKPLSELAKAMTAVPQVLLNVKVSKKPNLDSIPD
                     LQQAIRESERRLNGSGRVLVRYSGTEPLLRIMVEGEQDTVIREEADRLATIITAHLG"
     gene            complement(7339..8202)
                     /gene="folP"
                     /locus_tag="VLD60_03620"
     CDS             complement(7339..8202)
                     /gene="folP"
                     /locus_tag="VLD60_03620"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSB44072.1"
                     /translation="MEFQAGNQLIGLEERPLLMGIVNVTPDSFSDGGCYFDCEAAVAH
                     AIQLVKEGADLLDIGAESTRPGALPIDATEELRRLIPVVTRVAKAVSVPISVDTSKAS
                     VAKAAIEAGAAIVNDVTALRGDPAMVDVVAQAESGLVLMHMKGSPQTMQEAPQYHDVV
                     EDVAAFLSERARHAIERGVARNRIILDPGIGFGKVLVQNLDLLAQLPRFTQLGYPLLV
                     GPSRKSFIGQLTGQPVKDRGWGTAAVVALAVEQGVQILRVHDVAAMRDVVQVASAIVR
                     RRKSLLREQHA"
     gene            complement(8407..10218)
                     /gene="ftsH"
                     /locus_tag="VLD60_03625"
     CDS             complement(8407..10218)
                     /gene="ftsH"
                     /locus_tag="VLD60_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSB44073.1"
                     /translation="MNSRAKNLLFWVVVGLFMILLFNLFSVPTHAPEEEVIFSDFMAK
                     LDKGDIEKVIIKSSHVSAVLKDKTRIRTYTAEYPDFVKVLREKGVQIEAKPPDESPWY
                     ITFLVTWGPFVLFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGID
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRSEILK
                     VHTKKVPVAANVELEKIARGTPGFSGADLENLVNEAALWAARQNKKEVEVVDFEMAKD
                     KVLMGAERKSMILSDEEKRTTAYHEAGHALMAKLLPGTDPVHKVTIIPRGRALGVTMQ
                     LPTDDRHNYSKEFLYNNLAILMGGRVAEELILKHITTGAGNDIERATDLARKMVCEWG
                     MSEKLGPLTFGRKEEEIFLGRELTTKRDFSDQVALEIDLEIKRLVTENYERAKRLLTE
                     HLTSLKALAEALLEKEVLDATEIDQILLQSSSQTVPA"
     gene            complement(10321..10866)
                     /gene="hpt"
                     /locus_tag="VLD60_03630"
     CDS             complement(10321..10866)
                     /gene="hpt"
                     /locus_tag="VLD60_03630"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSB44074.1"
                     /translation="MSMDRIFGRPIVTQEEMRARIKELGKQIAADYAGKDFVLVGVLK
                     GAYAFYADLARAIRIPMRIDFMIVTSYGSQAKTSGKVKVITELTEDIKGKDVLLVEDI
                     VDSGLTVQYLIKRLSAHKPKSIKVCTLLSKPERRTIDVPLDYVGFKIPNEYVVGYGLD
                     YQQKYRNLPYLAVLDLKGEQE"
     gene            complement(10868..12337)
                     /gene="tilS"
                     /locus_tag="VLD60_03635"
     CDS             complement(10868..12337)
                     /gene="tilS"
                     /locus_tag="VLD60_03635"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSB44075.1"
                     /translation="MVVTQINRRTKPALLKRMLRTIREQRLFAPGHHLLVAVSGGPDS
                     TALLSLLSRLAPSWRLTLTAVHFNYRLRRSESDEDEAFVAAFCRERQIPLIVQRPSLT
                     KRRRISSLQALARDARYGAMKSIAREIGADRIVVGHTANDQAETVLMWMLRGAGLTGL
                     AGMPFVRENLIVRPLLTATRDEIVEYLKQEGLPYRQDSSNETSSYRRNRIRRELVPVM
                     MQIAPSIIHVLQRQAEVLREDERYLEKAADALYATAVERELPGGQRLERQTFAALPVA
                     LQRRLVRMVLRATDGEKRSPSLPVVETVRRFFLSGAQGARLSLRHAELTRDREKVRID
                     PKGQRWQEDAKVSGTCTDLEVEVSIPSTAYWPRTKQHIHVQILPREEAEPFLKKPSSS
                     RAVFDADRCSGPLLLRNWRPGDRLYPCGMKGKSKKLQDLFTDWKVARCDRQAMPLLAA
                     REGILWVIGRRQDERFLVRACTSRCLLVTITESTSEGAL"
     gene            complement(12337..>13250)
                     /locus_tag="VLD60_03640"
     CDS             complement(12337..>13250)
                     /locus_tag="VLD60_03640"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HSB44076.1"
                     /translation="RSRPYPVATIGNFDGHHLGHRALLQTVVETARKAQGTALVLTFD
                     PHPVKILAPHVDLRFLTSPEEKLAHFELAGVDEVVFLEFTPALAAMTPEEFAESTLHR
                     QLKIAEIFVGEHFAFGKGRAGRIADLARFGDTYGFKVHPIAPITLNGGVVSSTRIRQL
                     IQAGDMEEATALLGRAYGISGRVVSGRQQGQAMGWPTANLPVPPHRVMPPDGVYAARA
                     SCESTTYDAIAYIGTRPTFGPGERLIEVNLLDQQHDLYGRELMVEFLERLRGDHTFAS
                     AEDLSAQISRDVVRAKMSCRRYSRGTQ"
BASE COUNT         2947 a         4002 c         3414 g         2887 t
ORIGIN      
        1 ccgttcattc acgaggatat cgccacgccg ccctgtctgg acaccggctc gatccaaacc
       61 aatcccccgg ctgttgaatc cgcggcccac cgagacaagc agcttctcga cttcaaagct
      121 cgtgccgtct ttgcaacgga ccaatatcgt tgtcgggtta cgattaaggg attcgaccgt
      181 tgttccggtc atcaccgtca cgtttcgctt cttcagttca cgcgtcattg tggaagagat
      241 ctcttcgtct tccagcggga gaatgcgctc catcaactcc accactgtca catctgtccc
      301 gagaccgcta tacagacagg caaattcgca gccttctact cctccgccga tgattaacat
      361 tcgagccggc acctgttgaa ggtccagtaa ttgctggctc gtcagaatct gcactccatc
      421 gatggggaac tgcggaaggt tcggccatga cgaaccggtt gcgatcacta tggcatcagc
      481 cgtgaccact tgctctgtcc cctccttcat ggaaacgcgc acagtcccgg ggccttcgat
      541 cacgccggtt ccttcaatgt gctcgatatt ccaggcattg aagagtgtgg caatcccttt
      601 gaccaagcca gccaccacct gattcttcct cgcgaccatt tgagctagat cataggcaac
      661 tggtccggag atctgaatcc caagcggccc tgctttctta attttgtctc cgagttcgac
      721 gacagagagc aatgccttgc tgggaatgca gccccagttt aaacagacgc cgccgagtgc
      781 ctgggattcg acaacggtca cacgcgcgcc aagctgggcg gcccggatcg ccgcgacata
      841 cccaccaggc ccggcgccca gaatggcgat gtgcttagac atgggaacga atcaatgctt
      901 ttgcgtggag agaaaggcct cgtactgcgc tgccgtcatc aacttgtcga cttccccggc
      961 attggagaga tcgacgacta ccatccagcc cttgccatag ggatcgctgt tcacaacttc
     1021 ggggtgatcc ttcagatcgg cattcacttt tactatagtc ccactgactg gcgtatagat
     1081 actcgaagtg gttttggtcg attcgacttc tccaatttgc tgtccggctt tcacgaccgc
     1141 tccgattttc ggcaagtcga tgaatacgat atcgcccagg gcatcttggg cgaaatgact
     1201 gatgccaacg gtagcctgcg aaccgctgac acgtgcccac tcatgttctt gatggtatcg
     1261 aagatcagct ggaatcatgc tggtcctttc atgctctgac gcccaaggaa cgggccttag
     1321 cccaatatct ccatcccggg gaaaaagtag gtgatctcaa actgggccgt ctcgggtgaa
     1381 tccgacccat gcactgcatt gaattcgatg ttggctccgt gcgcctttcg aatcgtacca
     1441 gcttcggcct tcgccggatc tgtcgcaccc atcaactcgc ggtttttctt aatcgcattg
     1501 tccccctgca acacgagcac taccgatgga ccggaacaca tgaagctgca aagactgtcg
     1561 aaaaacggtc gggccttgtg gacagcgtag aacccttcgg ccgtcatttt cgacatctgc
     1621 atcaatttca tggcaacggg cttcagaccg gccttttcgt agcggttgat aatgtcgcca
     1681 atcgcgtgct tcttgaccgc atcgggcttg ataatcgcta aggttcgttc actcattgct
     1741 tgaactcttc ctccaatcta atgatgggac attgcaaaaa aatatttggt cattataggg
     1801 atggcccgcc ggagtttcaa gggacgggcg ccactaccca gcaaaatgtt ccgcaatttc
     1861 gtgaggcttg aagacgcccc gttcggtaat aattcccgca atgagttcgg cgggcgtgac
     1921 gtcgaaggcc gggttataga cagccacgcc cttgggagcg acagggtgac ttccatgaat
     1981 cgttgtgact tcaagcgggt tacgttgttc aatcggaatc tccgccccgg tttttgtttt
     2041 taagtcgata gtcgaatagg gtgcggccac gtagaacgga atgccgtgag ccttcgccag
     2101 aacggcgacg gaataggtgc cgattttatt ggccacatct ccgttggcgg caatgcgatc
     2161 cgcgccaacg acgcataaat gaatcttccc ttctcgcatg agcgatccgg ccatgttatc
     2221 agtgatcaac gtgacgggaa tcttgtcttg catgagttcc catgccgtca gcctggctcc
     2281 ctgcagcaca ggcctcgtct cgtcggcaat gacctgaatc ttcttcccct gctcccacgc
     2341 tgcgcgaatg acgcccagcg cagtcccgta accagcggtc gcgagcgcgc cggcattaca
     2401 atgggtcagt accgtctgcc cagtcgctat gagtgcagcc ccgtgccgtc ccatcgcttt
     2461 gcaaagcgcg atgtcttcgt caagaatcgc ctgagactcc ttcatgagcg ccgccttgat
     2521 tgccgcaacc gattcagttc gcaacgcggc gaccctctgc ctcatccgct caatggccca
     2581 gaagaggttg accgcggtcg gtcgagccgc agcaagacga tcgcagatgg caaacaatgc
     2641 agtggaaaat tcctgataat cagtcgcggc aattgattgc gcaccaagtg caactcccat
     2701 ggcagccgta acaccgattg ccggagcgcc cctgactttc aattcgcgaa tagcttgcac
     2761 gaccgcttcc acgtcgcgac aatccagaaa ttcgacctgc tcgggcagcc gactctgatc
     2821 gatcaaccgt accgcgccgt ctttccattc aacggtagga accataggag actcttgtaa
     2881 cgagaatctc tcgtgagtgc aagaggcagc acatgtcgat tttatgaagg agaatgggag
     2941 acgggcagga tcggctgaat acttctaaga gaggtcgatg aaacctgtgc gctaccggct
     3001 attggctcgt tttcccactg gaagcagcgt aacaggtttt tgttccgccc tttgatctag
     3061 ctttgccaat tcacgttcca gcatcgccct gataaaacca ctggtggtat agccctgctg
     3121 tttgagggtg tccaattgag tcttgagttc ctcaggtaat tgaatgacga ttcgtacgag
     3181 cttcggcata gcaagctccc ttcaacagca aaaacagggt gatccggccg aagtgattgc
     3241 acacgctatg ccttgctatc atttgcaggg ccttgaatag aattgaggac ttatgcgcat
     3301 ccagtgaaaa aagtatgagg aatcttgcct attttcgtct gccatcgccc tcaattgtac
     3361 aaccattgtg cgtaggaacc tgctcaaggc ccagccctac gtcactgcat ccattgcccg
     3421 gccaaacgcc gccagtttgc tatttcgcag tccaataggc aggcggataa ggctgtcgga
     3481 tgaagtttct catctctcgt acggtgaacc tgtaggatag aatttgatga cttcccggca
     3541 atccatattc caccgtccag gtctgttcga tagatcgatg ctccttgtcc ggcataggca
     3601 tcgacgacct tgggagcggg atgcccatag ggattatgtc ggccgaccga gaacacggca
     3661 tgctgtggac gtaacccccc aatccattcc ggattcaacg aactgatggc tccgtgatga
     3721 ggcaccttca agactgcgac cggttcccgt cgagacgtga gctgcattcg gcgaagacta
     3781 tcctgttcaa catcagcagc aaaaatcatc cggtgagccc cacaggacag ctcggtgaca
     3841 atggagcgat tattcaacag atgtccttcg cgatggttgg caggttgttg ctctcgagtc
     3901 acggcagcat ccggtggatt caacaccatg agtcggcagt catgggatga aaggatctct
     3961 tggccttcct tggcctgctg ttcagtaagt ccacgctctg caagcgcctg caaaagcctg
     4021 cgatagaaca attcgtcacg gccgtctccg gttccccagt aatgtttcac attgaagtgc
     4081 cgcagcagcc aggttagtcc tcccacatgg tcgagttggg gatgagttcc gaccatgtga
     4141 tcaatggttc tgattcccct gttccagagg tatggagcaa ccacaccacg tcccatgtcg
     4201 aatcgttcat atgtcgcccc tccatcgatc acaacgaccc ggccgtcagg ccactcaatc
     4261 accgtactgt caccctggcc cacatccaag aaggttacga gaaattgatc gccttcgagt
     4321 gcaatcctcg gagaccagat ccaccagagc aacaacagca taatcccagc cccggctgtc
     4381 attcggaata agacttgtct ccatctcagc cagatcaccc ccagacacgc ataaaacacc
     4441 agcatcgtga ggattgaggg ggaggcaacg tgccactccc ctcctggcat cctcgatacg
     4501 aagtgtatgc cctcgatgaa ccagtcgatt aaaagttgca gcagcgagct taatggcaag
     4561 acggtgcctc cactcatgat ctgacccagg cctgcaaaga gacccaacgg gacaaggagc
     4621 aaacccatca gtggaacagc gacgatgttc gtaacgagac ccagccacga cacctgattg
     4681 aagtagaagg cgacgagcgg cgtcgttgcc agcgtcacag ctgcgctcat gactacgacg
     4741 tctctgctcc acttcatgcc tgtcgcaagc gctgtcgcgt ggtgtgaggg ctccgcttct
     4801 tcagagggga gagaccaaga aagccaggca gcgattgcac agaccgaaag aaacgacagc
     4861 tgaaatgaaa tgtcagagag cgcctgagga tcgtgcagca atatcaccat ggcagccagt
     4921 gccagtgcat gaaacatgcg ctggtcatat ccaagccatt tggcgagcaa cgttgttagt
     4981 accatcacca gcgaacgcac agtcgccagc tctgctccgg ccagacaggc ataggcagtc
     5041 acggggacaa ctgtcgccgc agcggcaatg cgggtcgccg tgatcgttcg agacatcgcc
     5101 agcaaccatg cctgcggcag tcgcagtacg gcggctctca ccgaaacaaa tacgaggagg
     5161 gccaccaggc cgagatggct ccccgaaata gacagcaggt gtacggttcc cgtgaccatg
     5221 aactgatcgc gcagctcagc atccagaaat ccccgatcac cgacgatgat gccgagaaac
     5281 aaccccaacg ccggctgtga aagcgactgg agggccgcaa gccgaatgct ccctcgccag
     5341 cggtcaatgc gattccagac cctccaccag gctgacgcct gccctgattc cagaaattcg
     5401 accccgtcaa ctccggtagc ggtagccacg gcatcaatcc cctgccgctc gagataggtt
     5461 gcatagtcga accctccggg attcgacgtg ccgctcggaa cccgcagctt ggccttgaac
     5521 cggatgcggt caccctcgaa caggagccgc tctggcatac gccaggtcag tcgaatcagg
     5581 cgcggacgcc cgggcgccgt catctctcca tcaggctgga cgaccatgag caatcgatcc
     5641 ggcgcctgtt ggacaggggc catgatccgc cccgtgacag tctgaagaag attctgttca
     5701 ctctcgacga acacaacctt ggaaaacccc tcgactgcga caatccaata gatgattccc
     5761 agcaagagac aaccaaagcc ggctgtggca tggcttccca ccacgcgctg ccgtacttcc
     5821 aggatggaca atcctatggc gatggtaaaa agaaggagcg aaacggagaa ggggaaatac
     5881 gggatgaggg aaccgacgac gagaccacag gtgaaggcga gagttaggga cggtagcatg
     5941 ggctcccgtc gatcaggatg gctggacgac cagccagtct cgtatcaatc caacgcttag
     6001 ccgagatggg ccgtgatgat ggtcgccagg cgatctgctt cttctcgaat gacggtgtcc
     6061 tgttcgccct ccaccataat tcgcagcagc ggttcggtcc cggagtagcg gaccaggacc
     6121 cgcccgctgc cgttcaggcg gcgctcgctc tcgcgaattg cctgctggag atcgggaatg
     6181 ctgtccaggt tcggcttttt cgataccttt acattcagca acacttgagg caccgcggtc
     6241 atggccttgg ccaattcgga cagaggcttc tccgtccgct tcatcaacga taacacctgc
     6301 agagctgaaa tgagcccgtc gccggtcgta ttatgatcga ggaagatgaa atggccggat
     6361 tgctcgcccc cgaagttata gccatcggcc atcattcgct ccagcaaata gcgatctccc
     6421 acggccgttc gaaccagctg aataccggct cgcgtcatgg cgagctctaa cccaaagtta
     6481 ctcatgacgg tgccgacaac cgtctccctc gcgagcagcc cattccggtg aagatccagc
     6541 cccaaggcgg ccatcatgtg atcgccatcg acaacgtgtc cctgctcgca cacaaatatc
     6601 gcgcgatcgg catcgccgtc gagtgccact ccgagattgg cgttatgttc acgcaccgcc
     6661 ttctgcaaga gctcaggatg gaccgctccg catcccgcat tgatgttcat gccgtccggc
     6721 ttgttgccga tcacctcaac tttggcgcca agctcgcgca ggacggtcgg cgctactttg
     6781 taggccgcac cattggcaca atcaacgacc aatttgatgc cttgaaaatc gagttccttc
     6841 ggcaacgacc gcttgacgaa ctcgatgtag cgcccttcgg catcgtcgat tcgaaaggcc
     6901 ttcccgatcg cgtcggccgt cgggcgcaaa tgagcgatct cgttggacac gatgagctgt
     6961 tcgatccgcg cctcgatctc atcaggcagc ttcaacccgt cattggaaaa gaatttgatc
     7021 ccgttgtctt gatacgggtt atgggacgcc gaaatcatca cgccggcatc tgctcgcaag
     7081 cttctggcca ggaaggcgat cgcaggcgtc ggcattggtc caaccaatag cacatctacg
     7141 cccatcgaac agataccgga gaccaatgca gcctcgagca tataacctga aatgcgggta
     7201 tccttcccga tgacaatctg atgccggccg gctcggcgct tgaaaatatg cgccgcagcc
     7261 ctgcccagct gcatcgcagt ctcactggtc atcggctcga gattcgcaat accccgaacc
     7321 ccgtcggttc caaagagttt acgcatgttg ttcccgtaac aaggatttcc tacggcggac
     7381 gatcgcggat gccacctgaa ccacgtcgcg catcgccgcg acgtcatgga cgcgaaggat
     7441 ctgcacccct tgttccacgg ccagcgccac caccgctgct gttccccacc ctcgatcctt
     7501 caccggttgg ccagtcagtt ggccgatgaa cgactttctg gacggaccga caagcaatgg
     7561 ataacccaat tgagtgaatc ggggaagctg cgccaagaga tcgagattct gtacgagaac
     7621 cttaccaaac ccaattcccg gatcaaggat gattcgattt ctcgcgacgc ctcgttcgat
     7681 ggcatgtcgt gctcgttctg atagaaatgc ggcaacatct tcaaccacat cgtgatactg
     7741 cggtgcctcc tgcatcgtct ggggactccc cttcatatgc atcagcacca aacccgattc
     7801 agcctgggct acgacgtcaa ccatcgccgg atcgccccgt aatgccgtga cgtcattaac
     7861 gatcgccgct ccggcttcga tggcggcttt ggccacggat gcttttgacg tatccaccga
     7921 tatcggaacg gaaaccgcct tcgccaccct cgttacgacc gggatcaacc gtcggagttc
     7981 ctccgtcgca tcgatgggca aggcgcccgg tctggtcgat tctgcaccga tatccagcag
     8041 atcggcccct tccttaacaa gttggatggc atgagccacc gcagcctcac aatcaaaata
     8101 gcatccaccg tcggaaaagg aatccggtgt cacgttgacg atccccatga gcaacggacg
     8161 ctcttccaat ccaatgagct ggtttccagc ttgaaattcc atggtcttca gcgaaatgcc
     8221 cgcagcagat ccacatcaac tgttgcaggc cagatacaat ttcccaccgc agtgaatcga
     8281 ctccaacaga tctatcctgg cgaattatca cgaaagagga aagaacagcc tcttttaaat
     8341 ggctttacgt atgaacgccg tgcaactcca atgccctgaa gttgcacggg aggaacagca
     8401 ggatcgttag gcaggaaccg tctgcgacga agactgtaac agaatctgat cgatttccgt
     8461 tgcatccaat acttcttttt ctaaaagggc ttcggcaagg gccttgagac tcgtcaagtg
     8521 ctccgtcaga agccgcttgg cccgctcata gttctcggtc acgagccgct tgatctcaag
     8581 atcgatttcg agcgcaacct gatcgctaaa gtcgcgcttc gtcgttagct cgcggccgag
     8641 gaagatctcc tcctccttgc gtccgaaggt caacggcccg agtttctcgc tcattcccca
     8701 ttcacagacc attttcctgg caagatccgt ggcgcgctcg atgtcgttcc cagcaccggt
     8761 cgtgatgtgc ttgaggatga gttcttccgc cacacgcccg cccatgagaa tggccagatt
     8821 gttgtacaaa aactcctttg aataattgtg gcgatcatcc gtcggcaatt gcatggtcac
     8881 gcccagtgcg cggccgcgtg ggatgatcgt cactttgtgc accgggtctg tgcccggcag
     8941 cagcttggcc atcaacgcat gacccgcctc atggtaagcc gtcgtccgtt tctcttcgtc
     9001 gctgaggatc atgctcttgc gttctgcgcc catgagcacc ttgtccttcg ccatctcgaa
     9061 atcgacgact tccacttctt tcttattctg acgcgccgcc cacagagccg cttcgttgac
     9121 caggttttcc aaatccgcgc cggagaatcc cggcgtccca cgggcgatct tttccagttc
     9181 gacattggct gcaaccggaa ctttcttcgt gtggactttg agaatttccg aacggccgcg
     9241 caagtccggc cgattcacga cgacttggcg atcgaaacga cccggtctca gcaaggcggg
     9301 atcgagcacg tcgggacggt tcgtcgcggc gattaagatg acaccttccg ttgtatcgaa
     9361 tccgtccatt tcaacgagca gctgattgag tgtctgctcg cgctcgtcgt gaccaccgcc
     9421 gagtcctgca ccgcgcaacc gtccgacagc atcgatctca tcaatgaaaa taatgcaggg
     9481 tgcatgtttc ttcccttgct cgaagagatc gcgcacacga gaggcgccga cgccgacgaa
     9541 catttcgacg aaatccgatc cgctgatgct gaagaacggg acacccgctt ccccggcgat
     9601 cgccttggcc aacagcgtct tgccggtccc aggaggtccc acgattaaca cgcccttcgg
     9661 gatgcgcccg ccaagcttct ggaacttgcg cggatccttc aggaactcga tgatctcgag
     9721 cacctcttct ttcgcttcat cgatgcccgc gacgtccgag aacgtcacct tcttacgttc
     9781 ttccgtcaac atccgggccc ggctctttcc aaaagaaagg gctttatttc ctccgatctg
     9841 catttgacgc atgaggaaaa accacaatcc caggaagagg acaaacggtc cccaggtcac
     9901 cagaaaggta atgtaccaag ggctctcgtc aggcggcttg gcttcgatct gaacgccctt
     9961 ttccctcaac accttcacga agtccgggta ttctgcagtg tacgttctga tacgggtttt
    10021 gtctttgagt accgcgctga catgactgga cttgatgatc accttttcga tgtcaccctt
    10081 gtcgagtttg gccatgaagt cgctaaaaat gacttcttct tccggcgcat gggtcggtac
    10141 gctgaagaga ttaaacagga ggatcatgaa caggccgact acgacccaga agagcagatt
    10201 cttcgccctg gaattcatcc agttctcctt gtgggtagca ctgtcaactg gtgagtaaaa
    10261 ccgcgaactt gagagatgtt aacataggct tcttcctggt gacaaccgcc ttcgcattcc
    10321 ctactcctgc tctcccttca aatcgagtac cgcaaggtag ggcagattcc gatatttctg
    10381 ttgatagtca aggccgtacc ctaccacata ctcgttcggg atcttgaatc ccacgtagtc
    10441 gagcggcaca tcaatggtcc ttcgttccgg cttgctgagc aaggtacaga ccttaataga
    10501 cttgggcttg tgcgcggaca ggcgtttgat cagatattgc acggttagcc cggagtcaac
    10561 aatatcttcc accaacagga catccttccc tttgatgtcc tccgtcaatt ccgtgataac
    10621 cttcactttc cctgacgtct tggcttgact cccgtagctg gtcacgatca taaaatcaat
    10681 tcgcatcgga atcctgatgg cgcgggcaag gtccgcgtaa aacgcgtagg cccctttcaa
    10741 cacgccgacc agcacaaagt ctttgcctgc gtagtccgcg gcgatctgct tgccgagttc
    10801 tttaatgcgg gcacgcattt cctcttgtgt cactatcggc cgcccaaaaa ttcggtccat
    10861 gctcatgcta tagggctcct tcgctcgtcg actctgttat cgtcacaagc aggcaccgtg
    10921 atgtacatgc ccgcacgaga aaacgttcat cttgccgccg gccaataacc cagagaattc
    10981 cctctcgagc agccaataaa ggcatggctt gccggtcaca tcgggccact ttccaatccg
    11041 taaacaaatc ttgcagcttc ttgctcttcc ccttcatgcc acagggatac agccggtcac
    11101 ccggtcgcca gttccgaagg agcagcggac ctgaacaacg gtcagcatca aagaccgccc
    11161 gggatgaaga cggctttttc aagaatggct cagcttcctc tcgtggcagt atttgtacat
    11221 gaatatgctg cttggttcta ggccaatatg ccgttgaggg aatcgacact tcgacttcca
    11281 agtctgtaca ggttcctgaa actttcgcgt cttcctgcca cctttgccct ttgggatcga
    11341 tcctgacctt ctcccgatct cgtgtcagtt cagcatggcg aagggataat ctcgcgccct
    11401 gtgccccaga gagaaagaac ctccgaaccg tttcgacaac cgggaggctc ggcgaacgct
    11461 tctccccgtc cgtggctcga agcaccatgc gaacgagtcg tcgctgcaga gcaaccggca
    11521 gtgcggcaaa ggtctgtcgc tctaggcgct gtcctccggg caactcccgt tccacagcgg
    11581 tggcatacaa cgcatcagcc gctttttcca agtagcgctc gtcctcacgc agcacctcgg
    11641 cctggcgctg cagcacgtgg atgattgacg gcgcaatctg catcatcacc ggtaccagtt
    11701 ccctcctgat acgattgcgc cggtaggaac tggtctcatt gctggaatct tgacggtacg
    11761 gcaatccttc ctgtttcaaa tactccacaa tctcgtcgcg ggtagcagtc aagagcgggc
    11821 gcacgatcaa attctcacgg acgaacggca tccccgcaag acctgtcaat ccggcaccgc
    11881 gtagcatcca catcaaaacg gtttcagcct ggtcattggc tgtatggccg acgacgatac
    11941 ggtcggcgcc gatctctcga gcgatcgatt tcattgcccc gtagcgagcg tcacgggcca
    12001 gcgcctgcag cgaagaaatt ctccgccgtt tggtaagcga tggccgttga acgatcagag
    12061 gaatctgccg ctcacggcag aatgcggcaa cgaaggcttc gtcctcgtca gattcgcttc
    12121 tccgcaaacg atagttaaag tggaccgcgg tgagcgtgag ccgccacgac ggggcaagcc
    12181 tgctaagcaa ggaaagcaat gcggtagaat caggtccgcc tgagaccgcc acaagcaaat
    12241 ggtgccccgg agcaaacagg cgctgttcac gaatggtccg cagcattcgt ttcagcaatg
    12301 caggcttggt tcgacggttg atctgggtca ccaccattat tgggttcctc gcgaataccg
    12361 acggcaactc atcttggccc gaaccacatc acgagaaatt tgggcggaca ggtcttctgc
    12421 cgaagcgaac gtgtggtctc cccgcagacg ttcaagaaac tccaccatca gctcccgacc
    12481 gtacaaatcg tgctgctgat ccagcagatt gacttcgatc agccgctcac ccggcccgaa
    12541 cgtcggtctc gtgccaatgt aggcaatcgc atcatacgtt gtcgattcgc agctggcccg
    12601 ggccgcatag accccgtcgg gcggcatgac ccggtgcggc ggaacaggga gattcgcggt
    12661 cggccagccc atagcctgcc cctgctgccg ccctgagacg accctcccgc tgattccata
    12721 ggcgcgaccc aacagcgccg tcgcttcttc catgtctccg gcctggatca gttgcctgat
    12781 tctggtggaa ctgacgaccc cgccgttcaa cgtgatcggc gcaatcggat gaaccttaaa
    12841 cccgtaggtg tcaccgaatc ttgccaggtc ggcaatacgg ccggctcggc ctttcccaaa
    12901 ggcaaaatgt tcgcccacaa aaatctccgc aatcttcagc tgccggtgaa gcgtggactc
    12961 ggcaaattcc tcgggggtca ttgcagccag agccggagtg aactcaagaa acacgacctc
    13021 gtcgactccg gccaattcga aatgcgcaag cttctcttcc gggctggtta gaaagcgcaa
    13081 atccacgtgg ggagccagaa tcttcaccgg gtgcgggtca aacgtcagga cgagcgccgt
    13141 gccctgagcc ttccgagccg tctccacgac ggtctgtaag agcgcacgat ggccgagatg
    13201 gtgaccatca aagtttccga tcgtggcgac tggatagggc cgtgacctgg
//
LOCUS       DATIFR010000063         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_615583, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000063 DATIFR010000000
VERSION     DATIFR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615583"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VLD60_03645"
     CDS             complement(<1..331)
                     /locus_tag="VLD60_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44077.1"
                     /translation="MANQLAAAFELYRSALQKTWQSLLRGWMTIVAVIGFLFLLLLAQ
                     QLTAPLGLAGGFLLGAINALLVGATLSLIEQSISFARSLTFQDVLHSFGHYFWDVIGV
                     GFVLWLPL"
     gene            452..>2971
                     /gene="clpB"
                     /locus_tag="VLD60_03650"
     CDS             452..>2971
                     /gene="clpB"
                     /locus_tag="VLD60_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HSB44078.1"
                     /translation="MDMNRMTIKLQEALQSASSHAMRRSHQGMDVEHLLLALLEQEGG
                     VVPALCEQAGLSLPSVRNAAEQALTKLPQVQGAAGGPGQLHLTPRLNQVLTKAEDEQR
                     SLKDDFLSVEHVLLAMVQEGGLFKKLGLSRDKLLGALQHVRGHQRVTSQDPEGTYQSL
                     EKYGRDLTKFANQGKLDPVIGRDEEIRRVVQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AIRIVKGDVPEGLKQKRVVALDMGALVAGAKFRGEFEERLKAVLKEIQASQGQVLLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQT
                     VFVDQPSVEDTISILRGLKERYEVHHGVRIKDAALVAAAKLSHRYIADRFLPDKAIDL
                     VDEAAARLRTEIDSLPAELDEVSRKVMQLEIEREALRKEKDQASQARLATLEKELGEK
                     QRDLQALKTRWESEKASVSRLRKMREAIDEVKQAIEKAERAYDLNRVAELRYGEMPRL
                     ERELAIEQQQLGKKQGESRLLKEEVDEDDIAAIVSRWTGIPVSRLVEGETEKLLKLED
                     LLHQRVVGQDEAVRAVADAVLRARSGIKDPNRPIGSFLFLGPTGVGKTELARALAATL
                     FDDEGNMVRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPFSVILFDEIE
                     KAHHDVFNVLLQVLDDGRLTDSQGRTVDFKNTVLIMTSNIGSPQILEAQQQGSPYEDV
                     RKVVMDELRQHFRPEFLNRVDETVVFHPLGTDQLTKIVEIQLERLRARLTERRITLSV
                     TPAALRHLGERGYDPVYGARPLKRLIQQELETPIARQLVKGE"
BASE COUNT          720 a          785 c          906 g          560 t
ORIGIN      
        1 tcagcggaag ccagaggaca aagcccactc cgatgacatc ccaaaagtag tggccgaaac
       61 tgtgcaggac atcctggaag gtgagtgaac gagcaaagga gatcgactgt tcaatgaggc
      121 tcaatgtcgc cccgaccagc aaggcgttga ttgcgccgag cagaaagcca ccggccaggc
      181 ctaatggtgc ggtgagttgc tgggcaagca ggagcagaaa aaggaacccg attaccgcta
      241 caatggtcat ccagccccgg aggagggact gccatgtctt ctgaagggca ctgcggtaga
      301 gttcgaatgc ggcagccagc tgattcgcca taagggacaa tagtctggac gctcttccat
      361 ggtcaagccg ttctccatcc tccggcgctt gactttccct cccctgtgat tatgttgctg
      421 tcatcggcac acctatgagg agacggcgac aatggacatg aaccggatga cgatcaagct
      481 acaggaagcg ctgcaatcag cttcatctca cgccatgagg cgcagtcatc aagggatgga
      541 tgtcgagcat ctgctgctgg ccctcctcga acaggagggc ggtgtcgtac ctgccttgtg
      601 cgaacaagca gggctgtcgc ttccctccgt cagaaatgca gccgaacagg ctcttaccaa
      661 gcttcctcaa gtccagggag cagccggcgg tccgggccag ctccatctga ctccccgcct
      721 caaccaggtt ctgaccaaag cggaagacga acaacgatca ctgaaagatg attttctcag
      781 cgtcgagcat gtccttttgg ccatggtgca ggaaggcggg ttgttcaaga aattaggcct
      841 gtcacgagac aaactcctcg gagcgctgca acacgtgcgt ggacatcaga gagtgaccag
      901 ccaggatccc gaaggtacct accagtcgtt ggagaagtat ggccgtgatc tcacaaaatt
      961 tgccaaccag gggaaattgg atcctgtgat cggacgcgat gaggaaattc gccgcgtggt
     1021 acagattctt tcccggcgca caaagaataa tccggtctta attggggaac caggtgtcgg
     1081 caagacggcc atcgtcgaag gactggccat ccgcatcgtg aagggcgatg ttccggaagg
     1141 cctcaagcag aaacgtgtcg tggcactcga tatgggcgct ttggttgccg gcgccaaatt
     1201 ccgcggcgaa ttcgaggaac ggttgaaggc ggttttgaag gagattcaag cgtcgcaggg
     1261 gcaagtcctg ttgtttatcg atgagctaca cacggtcgtt ggagccggtg cggcagaagg
     1321 ggcgatggat gcggcaaacc tcctcaagcc gatgctggcg cgcggcgaac tgcacctgat
     1381 cggcgcgacg acattggatg aatatcgcaa gcatatcgag aaagatgcgg cgttggagcg
     1441 acgcttccag accgtgtttg tcgatcaacc atccgttgaa gatacgattt caatcctgcg
     1501 cggactgaag gaacgatatg aagttcacca cggcgtacgg atcaaggatg cggccttggt
     1561 cgctgccgcc aaactttcgc atcggtatat tgcggatcgg ttcctccctg ataaagcgat
     1621 cgatctcgtg gacgaggcgg ctgcgcgtct ccggacggaa attgatagtc tgccggccga
     1681 gctcgatgag gtttcgcgga aagtcatgca attagaaatc gaacgcgaag ccttgcggaa
     1741 agaaaaggat caggccagtc aagcgcggtt ggcgacgctc gaaaaagaat tgggtgagaa
     1801 acagcgagac cttcaggctc tcaagaccag atgggaatcg gagaaagcgt ccgtatcgcg
     1861 gctccgaaaa atgcgagaag caattgatga agtcaaacag gccatcgaga aggcggagcg
     1921 ggcctacgat ctcaaccgtg tggccgaatt gcgatatggc gaaatgccac ggctcgaacg
     1981 tgaattggcg atcgaacagc aacagctggg gaagaaacag ggagaaagcc gcctcctcaa
     2041 agaagaagtt gatgaagacg atattgccgc gattgtcagc cgatggaccg ggattccggt
     2101 ctcccggttg gtcgaaggtg aaacggaaaa attgctgaag ttagaagatc tcctccatca
     2161 gcgcgtcgtc ggacaggacg aagccgtccg agcggtcgcg gatgcggtgc tccgcgccag
     2221 atccggcatt aaagatccga acaggccgat cggctccttc ctcttcctcg gcccaaccgg
     2281 cgtaggcaag accgaactcg ccagagcgct ggcggcgaca ctttttgacg acgaaggaaa
     2341 catggtccgc atcgacatgt ccgaatacat ggagaaacac acggtcgcgc gtctcatcgg
     2401 agctcctccg ggttacgtgg gctatgaaga aggcggccag ttgacggaag ccgtgcgccg
     2461 ccggccgttc tccgtcatcc tgttcgatga aatcgagaag gcgcaccatg atgtcttcaa
     2521 cgtgctgctt caagtgctgg atgacggccg cctcacggat tcccagggcc gcacggtgga
     2581 ttttaagaac accgtgctga tcatgacctc caatatcggc agcccgcaga ttctcgaggc
     2641 gcagcagcaa ggctcgccct acgaagacgt gcgcaaggtg gtgatggacg aattgcggca
     2701 gcacttccgt ccggaattct tgaatcgcgt ggacgagaca gttgtattcc atccgctggg
     2761 cacggatcag ctcaccaaaa ttgtggaaat tcagttggag cggctgcgtg ctcgcctgac
     2821 tgaacgacgg atcacgctgt cggtcacgcc ggcggcgctc cgacatctgg gagaacgagg
     2881 atacgatccc gtgtatggtg cgcgaccgtt gaagcggctc attcagcagg agctggaaac
     2941 cccgattgcc cggcagttgg tgaaggggga g
//
LOCUS       DATIFR010000064         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_998727, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000064 DATIFR010000000
VERSION     DATIFR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998727"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /gene="metG"
                     /locus_tag="VLD60_03655"
     CDS             <1..421
                     /gene="metG"
                     /locus_tag="VLD60_03655"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HSB44079.1"
                     /translation="VSESPIPPQPTPVAPAPGTSTSLEATPAPAAPTQITIDEFMNIQ
                     LKTAKVLSAERVPKSEKLIKLQVSLGAEQRQIVAGIGKKYEPEALVGKTIIIVANLKP
                     AKLMGIESQGMVLAAGDSEVRGLATILEEVDPGTKVK"
     gene            418..1275
                     /locus_tag="VLD60_03660"
     CDS             418..1275
                     /locus_tag="VLD60_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44080.1"
                     /translation="MTRPVFIPLLLALTLLVPLPSSGLPAEDEPPPDPHLNDPHPTTI
                     LVRVVAQGAMVLGRAVGGARVTITDIETKQILATGIQQGEAGDQNQIMRTPHLMEEPL
                     YSSRPSAAFTTTLELRRPTLVEVTAEGPLAYPASLRRTSKTVLLIPGQDLTSDGIVLH
                     LHGYIVQIEHPKPGEPLIAKDDVKLRASIRTLSGAIVRPHGDWDSRKVRIYGDLLIGD
                     RIVERLQMFYSSDTASFEAPFFVPPYKDAPDGITLRVTATDVSTGNSGLGTANYSVLT
                     ERLPPTKRN"
     gene            1447..2190
                     /locus_tag="VLD60_03665"
     CDS             1447..2190
                     /locus_tag="VLD60_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB44081.1"
                     /translation="MKLSIIIPAYNEEATIESVVRRIEAVDLGPIKKEIIVVDDASRD
                     RTREVLKQLRNVRAIHHPMNSGKGAALTTGIKAATGDIVIFQDADLEYAPEDYPLVIK
                     PLVGKDCDAVMGSRFLRERPVFWGKRKSPYLNHYIGNLLILWVTNTLYRQRFTDYEGC
                     YKAFYRSTLLETPIQATGFEFDNELVCKLMRKRIRIAEVPIQYSPRTYAQGKKITWRH
                     GLTMLWTIIKWRFLPIESAEVVTAREMAA"
     gene            2279..>3085
                     /locus_tag="VLD60_03670"
     CDS             2279..>3085
                     /locus_tag="VLD60_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HSB44082.1"
                     /translation="MSLGILKVAAVLEQEGITVELVDLSGVKNYEEALADHLAQSQTQ
                     CFGLTATTPQMPAATRIQEIIRRLRPSARIILGGPHVTLVYAAAKYERKRSVIGRAAR
                     SIRQLTAMFDVLVTGDGEVAVFEALQVSPPRIVDGDDPKSQLFLNNAALEEMPFPARH
                     LVDVSTYQYSIDGTRALSMIAQLGCPFGCGFCGGRMSPFLRRVRTRSSENIVREMVHL
                     HHTYGISGFMLYDDELNVNPKLVSLMSLIANTQQQLGVEFRLRGFIKAELF"
BASE COUNT          731 a          914 c          794 g          646 t
ORIGIN      
        1 cgtgagcgaa tccccaatac ctccacaacc gactcccgtt gcaccggcac cgggaacatc
       61 taccagcctc gaagcgacac ctgccccagc tgcaccgacg cagatcacga tcgacgaatt
      121 catgaatatt cagctgaaaa cagcaaaagt gctcagcgct gagcgtgtcc cgaaatcgga
      181 aaagctgatc aagcttcaag tctcactcgg cgccgaacag cgtcagatcg tcgcgggcat
      241 cgggaaaaag tatgagccgg aagcactcgt cggcaagacc atcatcatcg tggccaattt
      301 gaaaccggca aagcttatgg gcatcgaatc gcagggaatg gttctcgccg ctggcgacag
      361 cgaagtccgc ggcctggcca ccattctgga agaagtcgac cccggcacca aagtcaaatg
      421 acgaggcctg tctttatccc gcttctgttg gccctgacac tcctcgttcc tctaccttcc
      481 tccggattgc cggcggaaga tgaacctcca ccagacccgc atttgaacga tcctcaccct
      541 acgacgatac tcgttcgggt cgtagcccaa ggcgcgatgg tactggggcg agcggtcgga
      601 ggagcgcgcg tcactattac ggacatcgaa acaaagcaaa tcctggccac tggcattcaa
      661 caaggggagg ccggtgatca gaatcagatc atgcgcacgc cgcacctcat ggaagaaccg
      721 ctatacagtt cgcgtccatc cgcagcattc accacgacct tggaacttcg tcgaccgaca
      781 ttggttgagg tgactgcgga gggtcccctc gcctaccccg cctcattgag gcgcaccagc
      841 aagaccgttc tgctcattcc agggcaagat ctcaccagcg atggcattgt gcttcatctc
      901 cacggctata tcgttcagat cgagcacccc aaaccaggcg aaccattgat cgccaaggat
      961 gatgtcaagc tgcgagcctc cattcgaacc ttgtccggcg ccatcgtccg cccgcatgga
     1021 gattgggact ctcgaaaggt tcgcatctac ggcgatctct tgatcggcga ccgcatcgtc
     1081 gaacggctcc agatgtttta ttccagcgat accgcaagtt ttgaagcgcc cttcttcgtt
     1141 cccccctaca aagacgcccc ggacggaatc accctgcgcg tgactgccac agatgtttcc
     1201 acaggaaatt ccgggctggg aacggccaat tattcggtgc tgaccgaacg cctccccccc
     1261 acaaaaagga attaggccgc aggtcccctc gttctggctt gaatctcagc ccctcctcca
     1321 cacgaacaaa gcaaattccg tcccatgcaa attggctgat ttgattgaca aaaaatcggg
     1381 cctgggtgac ccttcagaaa ggttggcatc caccttattc actcataacc tggaacatac
     1441 gaacgcatga aactttcaat aatcatcccg gcctataacg aggaagctac gattgaatcg
     1501 gtcgtgcggc gaattgaagc cgtcgatctc gggcccatca agaaagaaat catcgttgtc
     1561 gacgatgcct cacgagatcg cacgcgtgag gtcttgaagc aactccgaaa cgtgcgggca
     1621 atccatcacc ctatgaatag cgggaaagga gccgccctca ctaccggcat caaggcagcg
     1681 acaggcgaca ttgtgatctt tcaagacgcc gaccttgaat acgcgccgga agactacccg
     1741 cttgtcataa aaccactggt tggcaaggat tgcgacgccg tgatgggatc ccgctttctt
     1801 cgtgaacgcc cggtcttctg gggaaagcgc aaatctccct atctcaatca ttacatcggg
     1861 aatttgttga tactctgggt caccaacacc ctctatcgcc agaggtttac cgactatgaa
     1921 ggttgctaca aagccttcta ccgctccacc ctcctcgaaa cgcctatcca agccaccggc
     1981 tttgagttcg acaatgaact cgtgtgcaaa ctgatgcgga agagaatccg cattgccgaa
     2041 gttcccatcc aatacagccc gcggacctat gctcagggga aaaagatcac ctggcggcat
     2101 ggcttgacta tgctctggac catcatcaag tggcgattcc tgccgatcga gtcagccgag
     2161 gtcgttacag ccagggaaat ggccgcatag tcaatacgct caacaggcac agcagatggt
     2221 caaacggatt tgtctcatta tcccgccctc ccttttcctg cttgatgaac gagtttttat
     2281 gtcgcttggt atcttgaaag tggctgcggt gctcgagcag gaagggatta cggtagagct
     2341 ggtggatcta tcgggggtga agaattatga ggaggcgctc gctgaccatc tcgcgcagag
     2401 ccaaacccag tgtttcgggc tgacggccac aacgccgcaa atgccggctg cgacaaggat
     2461 tcaggaaatc atccggcggc ttcgtccatc ggcgcgaatc atcctcggcg ggccccacgt
     2521 gacgcttgtc tatgcggccg ccaaatatga acgcaagcgg agcgtcatcg gacgagcagc
     2581 ccgatcaatc aggcaactga ccgccatgtt tgatgtcctc gtcaccggag acggcgaagt
     2641 ggctgtcttc gaagcgctcc aggtttcgcc tccaagaatc gtggacggcg acgatccgaa
     2701 gtcgcagtta ttcctcaaca atgcggcgct agaggaaatg ccgtttccgg cacgtcatct
     2761 ggtcgatgta tcaacctatc agtacagcat cgatgggacg agagctctct ccatgatcgc
     2821 acaacttggc tgtcctttcg gctgtggatt ctgtgggggc aggatgagcc cgtttctacg
     2881 ccgggtccgt acccgctcgt ccgagaacat cgtcagggag atggtccatc tccatcatac
     2941 gtacggcatc tcagggttca tgctgtatga cgacgaattg aatgtgaacc ccaagctcgt
     3001 ttcgttgatg agcctgattg ccaatactca acagcaactc ggggtggaat tccgtttgcg
     3061 tggctttatc aaggccgagt tgttt
//
LOCUS       DATIFR010000065        24962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1765510, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000065 DATIFR010000000
VERSION     DATIFR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24962
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765510"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /locus_tag="VLD60_03675"
     CDS             <1..1999
                     /locus_tag="VLD60_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSB44083.1"
                     /translation="SLIVTPLITFIVMQRVGLSANLMTLGGLAIAIGEIADGSLVVVE
                     NVYRHLAQSNGATQVSKLDVILHATKEVGRPILFGILIISVVFLPLMTLHGMEGKMFA
                     PLAYTLVIALLASVVVTLTLSPVLASLLLRGDHPEETRLTIWLKQRYLPVLRWTLRHR
                     GPVLFGSTAIVLISVVLVPFIGREFIPLLEEGALTPQVVRLPSVSLAESIEMEKQTHK
                     AMLEFPEVRMAVSKIGRPDIAYGPEEPNESDPIVSLSDRSTWKTATTQPQLTDAIRKK
                     LAEIPGISVLMSQPIQERVDELISGIRTECAIKLFGDDLDLLHDKAEEIAALLQQING
                     VKDVKVEQIAGQPYLTVDIDRQKIARFGINVADVQEIITTAIGGKVATHVYEGERRFQ
                     LALRFPELQRNSINTIGEIRVKSASGALIPMSDLATIEMREGPARISRQQVKRRIYIG
                     FNVVGRDIGSVVDEGRKKLAAQIRLPEGYTITWGGAFENMERANARLLIVVPITLGLV
                     FFLLFWAFRSLRYATLIILNLPFALIGGFVSLWWSGQYLSVPASIGFIELFGLAVGNG
                     IVLVSYINQLRQEGKQTDDAIITGCSLRLRPVVMTMMTTLLGLLPLALAQGIGAEVQR
                     PLATVVIGGLFTSTALTLVVLPALYRWFEEKADAKFAPEWV"
     gene            2011..3015
                     /locus_tag="VLD60_03680"
     CDS             2011..3015
                     /locus_tag="VLD60_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44084.1"
                     /translation="MTWLFFIGSAAVIVLAGTKLSRYGDQIAEFTGLGRLWIGVVLMA
                     AATSLPEVFTTISAGWLNAPDLAAGDLFGAGMSNMLTLGLIDLLHRQKRVWQQAALGH
                     TLTAGLAMVLTGLAAFFVLLKVNVTHVGVGLGSLTLFILYVLGMRLVFRQEDMERRQR
                     EQEVLIEGTIAKQDAVSRRAELRRAVIGFSLGAFALLVAAPFLAWSAERIAEETGTSS
                     TFIGTSLVAITTSLPELVTAIAAVRLGAFDLAVGNLFGSNAFNMAAFLFADLAYREGG
                     LLGSVSSAHALTALWSILMMNIGLMGIIYRAEKRFMLIEPDSLLMIVSYVLGLWLLFR
                     "
     gene            3030..5885
                     /locus_tag="VLD60_03685"
     CDS             3030..5885
                     /locus_tag="VLD60_03685"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HSB44085.1"
                     /translation="MEARNDAPAARPWHSIPADALAMELRTNLEAGLSAAEADRRQKS
                     EGFNELPEAPPPSPLKLFLSQFTSVIVWVLIGAAVVSGLLAEWLDAAAIIAIVFLNGL
                     LGFVQEFRAERSLAALRKMSVAMARVVRDGILRQIRASELVPGDVIHLEAGDRVPADA
                     RLIYSTNFQTQEASLTGESTPVQKDVRVLTGPDMPLADRTNMAFMGTIAVSGKARALV
                     VSTALRTELGRIATMIQQASEAERTETPLQRRLEQFGYTLLWLALAVVAVVFALGYWR
                     GEPLLEMFLTSVSLAVAAVPEGLPAVVTITLALGVTRMVNRHALIRKLPAVETLGSAT
                     VICTDKTGTLTKNEMTVTKLIVGDRAYDVTGEGYEPVGEIREASGVKKNGSELKVQSS
                     ELSGEGVNLRSDNSELKTQNSALQSLPPGLRELLTAAVLCNGASLREENGAWQVIGDP
                     TEGALLVAAAKATVTKQELEQAAPFEGEIPFDAERKMMTVIRRTGEGTVAYVKGAPDV
                     LLKCCTHRLTLDGVVEPLGDRERKAILESNASLAHESLRVLAAAHRRLDDAHANLQPE
                     TVERDLVFLGLFAMKDPLRPEAVQAVRLCREAGIKTVMITGDHKETAVAIARDLGLSD
                     GPGTAFSGAEINNLTDEQLTQFVERVTVYARVSAEHKLRIVQAWKRRGAIVAMTGDGV
                     NDAPAIKAADIGVAMGLAGTDVTKEASDMVITDDNFASIAAAVEEGRGVFDNIRKTVH
                     FLLSCNASEVLVMLFATLLGLPLPLLPIQILWMNLVTDGIPALALAVDPKAPDLMGRP
                     PRPPDGRLLDGRRLFAIGGEGLILGMIALGAFSYSLYGLHQNLEQARTVVFTVMVVVQ
                     LVHAFNCRNERLSLFQIGVGTNRALLGAFLLSIAMQVAVLTAPPVSLVFKVAPLPVED
                     WALMGAMGVLPLVIIEAIKLLRRTRRPPSHYSRPVHH"
     gene            complement(5900..8437)
                     /gene="mgtA"
                     /locus_tag="VLD60_03690"
     CDS             complement(5900..8437)
                     /gene="mgtA"
                     /locus_tag="VLD60_03690"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HSB44086.1"
                     /translation="MNERPYWAMPADELLARLQSRPEGLHAEEAQQRQAIFAASRLKP
                     HQDIRPLYALLSQFRSPIILILLFAATVSLFLADRTDALIILTIILISALLGFWQEHG
                     AAKAVAALLALVRVKTEVWRDGRLIEVPVEEIVPGDVINLSAGSSIPGDGTVLESKDL
                     FVDEATLTGETYPAEKTASVVSAAAPLSKRTNSLFMGTHVVSGDAKAVIAHVGKDTEF
                     GHIASHVMLRPPETEFERGVRRFGYLLLEVTLLLVFAIFAINVYLQRPVLDSFLFSMA
                     LAVGLTPQLLPAIISVNLSHGAKRMAQQKVIVKRLASIENFGSMNVLCSDKTGTLTEG
                     TMRLHAALSLDGLPSERVLFHACLNATYETGFINPLDEAIRTQCICDLSGYRKLDEVP
                     YDFLRKRLSILVATPTTHLLIAKGAVEQMLAVCTRVELPDGTTVSIEDRSEKIRQQFQ
                     DLNRQGLRTLGLASRDMGQAEHIDKGHEAEMTFLGLLVFADPVRPDIAETIASLRRLG
                     VSLKIITGDHRLVASHVSQQVGMDHQRLLTGQDLRRMTDEALLQRVNDIDVFAEVEPN
                     QKDRIILALKHVGNVVGYIGDGINDAPALHAADVGISVDSAVDVAKDAADIVLLEKDL
                     AVLIQGVREGRKTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNLL
                     TDIPEMTIATDRVDHELIDQPRRWDIAFIRKFMLTFGLVSSVFDYLTFGALLWILDAS
                     PEQFRTGWFVESVISASAIVLVIRTRRPFFTSTPGRYLSLATLAVAAVTLLLPLLPTG
                     APLGLTPMPLSFLFLLAAILAGYVLTAEIVKRRFYANGRNSGAVSAP"
     gene            complement(8455..9351)
                     /locus_tag="VLD60_03695"
     CDS             complement(8455..9351)
                     /locus_tag="VLD60_03695"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSB44087.1"
                     /translation="MTITQNSEIAQKLREVALLLEEQGANRFRVRAYRTAADRLEHLE
                     VPVSRIVEQQGTDGLQALPGIGVSLARSIHTLIVTGRLPMLDRLRGHTDPIALLTSVP
                     GIGSAFAHRLHDDLHIETLEQLEMAAHDGRLADIEGVGPKKLQGIIDSLTARLGRVHR
                     PPQTVGGHQAGEPSIEELLDVDREYRESALAGTLQKITPHRFNPKREAWLPILHTQRG
                     PRHYTALYSNSALANQLRKTRDWVILYYDDDHGERQCTVVTGRSAPITGKRIVRGREA
                     DCTSFYHSPKAGAVAGDSDSRA"
     gene            complement(9514..10746)
                     /locus_tag="VLD60_03700"
     CDS             complement(9514..10746)
                     /locus_tag="VLD60_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB44088.1"
                     /translation="MPFLWLTILSALRILRRNPLRAGLTMLGIIIGIGAVVAMVSLGQ
                     GATASVQAEIASLGTNVLIIIPGATTVGGVRGGLGTISTLTVDDAEDIEKKVGSVTMV
                     MYGSRSVLQVIGESKNWSTVVLGTTPQFTDIRSWPVAQGNFFTQSDMESAAKVAVLGK
                     TVVQNLYEPGDEVVGSQIRIRNVPLRVIGVLESKGQSVTGQDQDDLVILPFTTAERKV
                     LGTKFLGTVGIILVATQTRHDIPVVVDDIKELLRARHHVHPSEEDDFTIRTMEDIAKT
                     IAGTSRTMMFMLMGIASISLIVGGIGIMNILLVSVTERTKEIGLRMAVGAKRAHILLQ
                     FLVEAIIMTAIGGVLGVGAGIGSARLLTTMIGWPTIINVQVVAAAFFFSLVVGVFFGL
                     YPANKASKLNPIEALHYE"
     gene            complement(10840..12177)
                     /locus_tag="VLD60_03705"
     CDS             complement(10840..12177)
                     /locus_tag="VLD60_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB44089.1"
                     /translation="MSTNPDEPTRHVPVTEVIPPHSADLPAHRDPDSSPAYVRRLPTS
                     VIPLPPRMSRSHLPWLISIIIFLAFMAVASWYWWNSTSSPVQYKTALVDRGPITAVVT
                     ATGTVNPVISVLVGSQVSGKIAELSADFNSVVTKGQVLARIDQQPFQARVRQARAAVK
                     SANGNLAKAKNLAAQRKRERDRMAVLRPESFVSQADLDLAETNYRDAEANVEVTQAQL
                     DQAEATLASAELDLGYTTIYSPVNGIVVSRNIDVGQTVAASFQTPTLFVIAQDLTQMQ
                     VDSNVSESDIGGVAEGKPASFRVDAYPKQFFQGTVTQVRNAPINIQNVITYDVVITVD
                     NRELKLKPGMTANVTIVTAKKEDPLRVPNGALRFRMPNVPVDRKISQVWVLDPAAAPR
                     QATITTGIADSLFTEVVGGPVKEGDKVIVGIETAEEQAQKKLPPGFDGGPRMR"
     gene            complement(12180..14981)
                     /locus_tag="VLD60_03710"
     CDS             complement(12180..14981)
                     /locus_tag="VLD60_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSB44090.1"
                     /translation="MPELSQPAIYKAPAEAIFKQLETDPGGLSLEEAKQRLSRYGPNV
                     LVEPDHYSLLRGLVHQFTHFLAVLLWIAAGLAFAADFMRPGEGMATLGWAILGVIIIN
                     AAFAFLQEYKAEHAVQALRRLLPDKAWVTRSGQSVEISRKEIVPGDLLILEEGERVPA
                     DARLVEATGMRVDNAALTGESKPKRRTAEAAEDGHWLDLPNLVFAGTTILSGHGRAVV
                     FATGMRSEFGKIANLTTTVETSLGPLQKEIIKVTRIVAAISLAMGTAFFVIGLWTGLG
                     FWISAIFGIGIIVANVPEGLLPTVTLSLAMGSQRMAKRNALVKHLSSVETLGCTTVIC
                     TDKTGTLTENRMKVDRFYADGLVIESREGCFFTRGRIVSNTDAERWQSLFDALLHCHN
                     AKRVRRSDGRFVVTGDPTEVALVQFTIDHGLAHRPPLRRMGELAFDADRKRMSTLHWS
                     EGRLIAFTKGAPESVLALCTKAQIHGDAVTMTPDDRARILSQSRSFAEQAYRVLAIAM
                     REVAHQPEHIEVETIEEDLTFLGLVAMMDPPHREVPDAIVKCRKAGVRVVMITGDHPL
                     TALAIARKIGLVPNGAVQQPIGFVPVIEGSQLDGMSEEQLRQLLTPSRSGEPDPIFAR
                     MAPRHKLLVVSTLKEMGQVVAVTGDGVNDAPALKKADIGVAMGIAGTDVAKEIADMIL
                     LDDNFATIVSAIEEGRAVYENIRKFTSYILASNVPEIVPFLGYGFIGMPLALTIPQVL
                     AVDLGTDMIPALGLGTERPLAGVMDTPPRPRTERLLNRPLLLRSYLFLGGIEAIVAMG
                     GFFLYLFANGWTWGTPLSWSSPLYKEATTVTFAGIVMAQVANVFACRSHRVPAYRLGW
                     FSNPLILWGIATELTILVLITYTPTGHEIFGTSSLPFWIFGPVALGALVLLFAEEGRK
                     IIANRAVHKPAYKPTQAVH"
     gene            complement(14974..15879)
                     /locus_tag="VLD60_03715"
     CDS             complement(14974..15879)
                     /locus_tag="VLD60_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB44091.1"
                     /translation="MRILCAVDGSEFSGWGIQALAALAGKEPEHVALLHVINKPVLRS
                     VKNRHPAAEKRAIAAMEKAGELVLREAERSAKVALGQAATGPGTKFHKVLAYGPIAST
                     VAQQARRWKADLILTGSRGLSDIKGFLLGSVSRRVASMAPCSLLVVKGPTPALGRVTL
                     AVDDSKHSRAAARFLRSQILPETAAVTIVSSAENPVTDLAARYLSESQLAELTEPVLE
                     RSASLVEALRDDFIKEGYSVTTRVHMDHVIDTIVKQVEADHADLLVIGSRGLTKSERL
                     HLGSVSESLLRHAPCSVLIVRGVRA"
     gene            16207..16728
                     /locus_tag="VLD60_03720"
     CDS             16207..16728
                     /locus_tag="VLD60_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44092.1"
                     /translation="MGKRFILVMAMWLGLQAGFVSAEGNTVARIGQSGEVLELEQQVG
                     GKVLKNYLPLHEKGGVRYFSAGVGLEERSAEYPAFSLKLVFTAGGKPFLAGVDVAIQP
                     AKGGAALRIPREQVEGPWLFVDLPSGIYDITATHGDRTQVLKGVNVSAGKQRIVYLRW
                     AEDRGSAGRLPAE"
     gene            16769..17212
                     /locus_tag="VLD60_03725"
     CDS             16769..17212
                     /locus_tag="VLD60_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB44093.1"
                     /translation="MPRKKKAGPAKAKDFDSITVGQVMEKRVQFARLKTKGDVIASLM
                     IEGFGAVPVVDGGRRLAGIVSEHDLLAAMDDGHELGRITAGDIMTGNPYSVRPETTVG
                     TLVHVLRASDLVRVPVVDAKDKLVGIIARRDVLRAYLAAGLSKRK"
     gene            17223..17657
                     /locus_tag="VLD60_03730"
     CDS             17223..17657
                     /locus_tag="VLD60_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB44094.1"
                     /translation="MRQTEFFMGACDPKTLTVGQLMQDAVFSCGPRTTASTLAHLMTH
                     RNFGSLPIAEEDGTLVGIVSEYDLLQAMIEGRDLRKILAVEVMSAHPVTVTEEQTLTQ
                     VADLFQDRYITRVPVVRNKKLVGILARRDLLFGYLKASQYWS"
     gene            17776..18588
                     /locus_tag="VLD60_03735"
     CDS             17776..18588
                     /locus_tag="VLD60_03735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSB44095.1"
                     /translation="MLLSRAIVGALMMATCLSVSVQADPVRTHRAPCEITYPSDATIE
                     WECRTLRSGESLEKLFGERWVDVARFNRIDRRHARAGISIKVPQRLDELVAFAPLPFF
                     YPPAEQDEQFILIDLSEQFLGAYEHGTLRFALPIVSGNGHKETPTGEFRLTAAHRAHQ
                     SCLYTVEGTDRPYPMNYALRFQVNREGVSYWIHGRDLPGYPDSHGCIGLFDEPMQKEQ
                     YGIPKDPELSDAKRLFDWVLGAEEQDERVIPLKHGPRVHIIGEAPKSGRESP"
     gene            complement(18780..19181)
                     /locus_tag="VLD60_03740"
     CDS             complement(18780..19181)
                     /locus_tag="VLD60_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44096.1"
                     /translation="MTRVRHVVLARRNLLLIFAGVIAMVESALGCPGVGEWRLPAELE
                     SKLESTAAAEDHLSAALIYQKQAEQVEITALKFEDSAQKITQLEDPKNFRRSGLKIAG
                     QQCRKEAIDLQRLADHHRQKAETLQANHQAK"
     gene            19771..19950
                     /locus_tag="VLD60_03745"
     CDS             19771..19950
                     /locus_tag="VLD60_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44097.1"
                     /translation="MDCPKCQGLMLLERFSDFFLVFYAWKCLNCGAIIDRTISNNRKN
                     SLAANAVKEVEAATV"
     gene            complement(20194..21621)
                     /locus_tag="VLD60_03750"
     CDS             complement(20194..21621)
                     /locus_tag="VLD60_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSB44098.1"
                     /translation="MRSASFGLSIAYVILTAVLTMSLTASVQAEEAGLTTFHGKPCTS
                     AHKKHTIPAKTLRAIVQSHGKWLDERHNPDYKRADLCQADLRQAVLIGANLEKANLEG
                     AQLQQANLYQSTLAQASLAGADLTKAGMEDSNISGADLRKARLSKVNLFRSVGDEAAL
                     FDAVLTGTQLHESTFERAHFEGADLSSADLTNASFVDSYFYGANLHRAILAGTDLMGA
                     DLRRTNLTKANMHQTNLQGALLDGAQLSGARLEEADLEGAYFDEADLTEANLREALLR
                     GADLRFTNLQSAGLQQADLEGVNFEGARLVKVNAQSANATMAILYHANLDEADFRDAR
                     LHHAVLIEARGEGAIFAKADLTEIYAPKVSLRRARFNEATLEAANLVAADLRGANFSH
                     AILAHANLQEANLQGALFHGADLSGARLDTADLRQANLRGANLTAVTGLSQPQLDSAC
                     LDEQTKLPPELKRPAPCTEKKSKKK"
     gene            complement(21867..23201)
                     /locus_tag="VLD60_03755"
     CDS             complement(21867..23201)
                     /locus_tag="VLD60_03755"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HSB44099.1"
                     /translation="MNPSTSALLTDLYQLTMIQAYLQQGMEEEAVFEFFVRKLPPHRN
                     FLLAAGLEQVVEFLAQLYVSTEEVAWLESTGRFTPALLKYLQGVRFTGTVDALPEGTI
                     FFPNEPILRIIAPLPLAQLLETRVMNLLNFQTMIASKAARSVLVSGGKPIIDFGLRRA
                     HGAEAGLLAARASYLAGFAGTATVLAGMQYGIPLYGTMAHSFVQAHKDEGVAFEHFAQ
                     AQPENVVLLIDTYDTEAGARRVVGLAPQLRTMGIPVKGVRLDSGDLAEHARNVRGILD
                     EGGLRDAQILASGNLDEYRLRDLTNTGAPIDSFAVGTAMTTSADAPSLDCAYKLQEYA
                     GRPCRKRSEGKATWPGRKQIYRRYASDGTLAHDVVTTLDDRQEGEPLLEPVMKEGRRL
                     THQPNLTDLRHRAATQLTRLPETMRTLETVTPYEVRIAPSLQRLAQVVDRAV"
     gene            complement(23198..23779)
                     /locus_tag="VLD60_03760"
     CDS             complement(23198..23779)
                     /locus_tag="VLD60_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSB44100.1"
                     /translation="MKSLDAHDALLVADIQNDFLPGGALGISGGNEIIPVLLTYIHRF
                     HSKSLPIFLTRDWHPPNHCSFRDRGGPWPVHCLAGSSGSLPPRSFEAPLSAVIIYKAI
                     DADQEAYSAFQNTPLHRHLQAIGVRRLFIGGLATDYCVLNTVKDARALGYDVCLLTDG
                     IRAVNVHPDDGRNAEQEMLRLGVAPIRLEALAV"
     gene            complement(23822..24181)
                     /locus_tag="VLD60_03765"
     CDS             complement(23822..24181)
                     /locus_tag="VLD60_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44101.1"
                     /translation="MRASLFCGFLLCGALAVPTALLAQDYPADITRGKAVYERHCLAC
                     HGTGGWGDGPNAASLKVAPANFHRFNSFLKSDEELLRTIEHGVVFSPMHSWRGQLTDG
                     EMQDVVAYIRLLSQQGR"
     gene            complement(24190..24354)
                     /locus_tag="VLD60_03770"
     CDS             complement(24190..24354)
                     /locus_tag="VLD60_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSB44102.1"
                     /translation="MPMYDYKCLDCGKESLIVLTLREHETGKVACPSCKSTNMRQLYT
                     SFIAHTTKKS"
     gene            complement(24372..>24962)
                     /locus_tag="VLD60_03775"
     CDS             complement(24372..>24962)
                     /locus_tag="VLD60_03775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSB44103.1"
                     /translation="IHSKGVMTLGGFLAGKFAGAHPFALSATLTFEQTYSEIEGDSAT
                     VAELVAILSSLADLPVRQWLAVTGSANQLGEVQPIGGVNEKIEGFFESCKKRGLTGKE
                     GVIIPSRNSKHLALRRDVVEAVEAGQFAVYAVGTVEEAIELLTGLPAGERGLDSLYPP
                     DTVYGRAAARLEEMARAVAEWGEGEMKSEGRIITES"
BASE COUNT         4890 a         7032 c         7742 g         5298 t
ORIGIN      
        1 ctcactcatc gtcaccccgc tgatcacgtt catcgtgatg cagcgggtgg ggctctcggc
       61 caacttgatg acgctcggcg gactcgccat tgcaatcggc gaaatcgccg acgggtccct
      121 cgtcgtcgtc gaaaatgtct atcggcatct cgcacaaagc aatggcgcga cgcaggtgag
      181 caagctcgat gtcattcttc atgccacgaa agaagtaggc cgaccgattc tgttcggcat
      241 tctgatcatc agcgtcgtgt tcttgccgct catgacgctg cacggcatgg aaggcaagat
      301 gttcgcaccg ctggcttaca cgctcgtgat tgcgctgttg gcctcggtcg tcgtcacgct
      361 cacgctgtcg ccggtcctgg cctccctgct gttgcgcggg gaccatccgg aagaaacgcg
      421 tctgaccatc tggttgaaac agcgctattt gccggtgctc cggtggacgc tccgacaccg
      481 cggccccgtc ctgttcggtt cgacagctat tgtgctgatc agcgtggttc tggtcccgtt
      541 tatcgggagg gagttcatcc ctcttctgga agaaggcgcc ttgactcccc aagttgtccg
      601 actgccgagc gtttcgctgg cggagtccat cgagatggag aaacaaacac ataaggcgat
      661 gttggaattt ccggaagtgc ggatggcggt gagcaagatc gggcggccgg acattgccta
      721 tggaccggaa gagccgaacg agagcgaccc gatcgtgtct ctgagtgatc gtagcacgtg
      781 gaagacagca accactcagc cgcaattgac cgacgccatc aggaaaaagc tggcggagat
      841 tcccggtatc tccgtgctca tgagccagcc gattcaggaa cgggtggacg agctgatctc
      901 cggtatcagg accgagtgtg cgatcaaatt attcggggat gatctcgacc tcctgcacga
      961 caaggcggag gagattgcgg ccttgctgca gcagatcaac ggcgtcaaag acgtcaaggt
     1021 agaacagatc gccgggcagc cctatctgac ggtcgacatc gaccggcaga agattgctcg
     1081 cttcggcatc aacgtggctg acgtacagga gatcatcacg accgccattg gcggcaaggt
     1141 ggcgacccat gtctacgaag gcgagcggcg gtttcagttg gcgcttcggt ttccggaact
     1201 ccaacgcaat agcatcaaca ccatcggcga aattcgggtg aagtcggctt ccggcgccct
     1261 gatcccgatg agcgacctcg ccacgatcga gatgcgcgaa ggtccggcgc gcatcagccg
     1321 ccagcaggtg aaacgccgca tctacatcgg cttcaacgtg gttgggcgcg acatcggcag
     1381 cgtggtcgat gaagggcgaa agaagctcgc cgcgcagatt cgcttgcccg aagggtacac
     1441 catcacctgg ggcggagctt tcgagaacat ggagcgggcc aacgcacggc tcttaatcgt
     1501 ggtgcccatc accctcgggc tcgtgttctt tctcctcttc tgggcgttcc gttcgctgcg
     1561 gtatgcgaca ttgatcattc tgaacctgcc gtttgccttg atcgggggat ttgtctcgct
     1621 gtggtggagc gggcaatatc tgagcgtgcc ggcgtcgatc ggcttcatcg aactgtttgg
     1681 gctcgcggtc gggaacggga ttgtgttggt gtcgtatatc aatcagcttc gccaggaagg
     1741 caaacagaca gacgatgcca tcatcaccgg ttgcagcctg cggctccgtc ctgttgtcat
     1801 gaccatgatg acgacgttgc tggggctcct gccgcttgcg ctcgcacagg gcatcggcgc
     1861 ggaagtgcag cggccgcttg cgaccgtcgt catcggcggc ctcttcacct caacggcgct
     1921 cacactggtc gtgttgccgg cgttataccg gtggttcgaa gagaaagcgg acgccaagtt
     1981 cgcaccagag tgggtgtgac cggagaatcg atgacctggc ttttctttat cggtagtgca
     2041 gccgtgatcg tcctggccgg gacgaagctc tcacgatacg gagatcagat cgccgagttc
     2101 acgggcttgg ggcggttatg gatcggcgtg gtattgatgg ccgccgccac gtcgcttccc
     2161 gaagtcttca cgaccatcag cgccgggtgg ctgaacgccc cggatctcgc ggccggcgat
     2221 ctgttcggcg ccggcatgag caacatgctg acgcttggcc tgatcgatct cctccatcga
     2281 cagaaacgag tctggcaaca ggccgcattg gggcatacgt tgacggctgg gctggccatg
     2341 gtattgacgg gccttgccgc gttcttcgtc ctgctgaagg tgaatgtgac ccatgtcggc
     2401 gtaggcctcg ggagtctcac cctctttatc ctgtacgtgc tgggcatgag actcgtcttc
     2461 cggcaggagg acatggaacg tcgccagcgt gaacaggaag tgttgattga aggaaccatc
     2521 gccaagcagg atgccgtcag ccgccgggct gagttgcgtc gtgcggttat cggcttctca
     2581 ctcggcgctt ttgcattgct cgtggccgcg cccttcctgg cctggtccgc cgagcgcatc
     2641 gccgaagaga ccggtaccag ctcgacgttt atcggcacgt cgttggtcgc catcacgacc
     2701 tccttgccgg agctggtgac tgcgatcgct gcggttcgat tgggcgcgtt cgatctggcc
     2761 gtggggaatc tgttcgggag caatgccttc aacatggcgg cgttcttgtt tgccgatttg
     2821 gcctatcgcg aaggcggctt gctgggcagt gtgagctcag cccacgccct gaccgcactc
     2881 tggtccatcc tgatgatgaa catcgggctg atggggatta tctatcgagc cgagaagcgg
     2941 tttatgttga tcgagcctga cagtttgctg atgatcgtca gctatgtgct gggtttgtgg
     3001 ctgttgtttc gatgaagcat ggagggggaa tggaagcgcg gaacgatgct cctgctgcga
     3061 ggccgtggca ctcaataccg gccgacgcgc tggccatgga gctgcgcacg aatctcgaag
     3121 ccgggctcag cgccgctgaa gccgaccgtc gacaaaagag tgaggggttc aacgagctgc
     3181 ctgaagcgcc gcctccctcg cctctgaagc tgttcctttc ccaattcact agtgtcatcg
     3241 tctgggtctt gatcggagcg gcggtggttt ccggtctgtt ggccgaatgg ctcgatgcag
     3301 cggcgattat cgccattgtg tttctcaacg gcctgctcgg attcgtgcag gagttcaggg
     3361 ccgaacgatc cttggcagcg ctgcgaaaga tgtctgtggc gatggcccgt gtcgtccgtg
     3421 acggtattct ccggcagatt cgagcgagcg aattggtgcc cggcgatgtc atccacctgg
     3481 aagccggcga ccgtgttcct gcagatgcgc gcctgatcta cagtacgaat ttccaaacac
     3541 aggaagcctc gctgaccggg gagtccacac cggtccaaaa ggacgtgcgt gtccttactg
     3601 gacccgacat gccgctggcc gaccggacca acatggcgtt catgggcacg atcgcggtgt
     3661 cgggcaaggc gcgtgcgttg gtcgtgtcga cggcactccg gacggagttg ggccggatcg
     3721 cgacgatgat ccagcaggct tcggaagcgg agcggacgga aactccgctc caacgccggc
     3781 tggagcagtt cggctatacc ttgttgtggc tggcgttggc ggtggttgcc gtcgtattcg
     3841 ctttgggcta ttggcgtggc gaaccgttac tcgagatgtt tctgacctcg gtgagcctgg
     3901 ccgtggcggc ggtgcccgaa ggtcttcccg ctgtggtgac aatcacgctg gcgttgggcg
     3961 tcacccgcat ggtcaaccgg catgcgctga ttcgcaaact gccggccgtc gaaacgctcg
     4021 gatccgccac ggtcatctgt accgacaaga ccggcacgct cacgaagaac gagatgacgg
     4081 tgacgaagtt gattgtcggt gacagggcct atgacgtgac aggtgaaggc tatgagccgg
     4141 tgggcgaaat ccgtgaggcg tcaggggtca aaaagaacgg ttctgagttg aaagttcaga
     4201 gttctgagtt gtccggagaa ggcgtgaatc ttcgatctga taactcagaa ctcaaaactc
     4261 agaactcagc acttcaaagc cttccccccg gcctccgtga actcctgacg gcggcggtgc
     4321 tctgcaacgg tgcgagcttg cgggaggaga atggggcttg gcaggtgatc ggcgatccga
     4381 cagaaggcgc gctgctcgtc gcagcggcaa aagcgacggt gaccaaacag gaactggaac
     4441 aagccgcccc gttcgaaggc gaaatcccct tcgatgccga acggaaaatg atgaccgtca
     4501 tccgtcggac cggcgaggga actgtggcct acgtcaaggg agcgccggac gtcctgctca
     4561 aatgttgcac gcatcgcctg acgctcgatg gcgtggtgga gccgttgggc gatcgggagc
     4621 ggaaggcgat cctcgagagc aacgcatccc ttgcgcatga gtcgctccgc gtgctggccg
     4681 cggcgcatcg tcgtctcgac gacgctcatg cgaacctgca gcccgagacg gtggaacggg
     4741 atctggtgtt tctcggcctc ttcgcgatga aggatccctt gcgccctgaa gccgtccagg
     4801 ccgtgcgtct gtgccgagag gccggcatca agaccgtcat gatcaccggt gaccataagg
     4861 aaacggcggt cgcgatcgcg cgggatcttg gcctctccga cggccccggc acggcgttct
     4921 ccggagccga gatcaataat ctcacggacg aacagctgac ccaattcgtc gagcgggtca
     4981 cggtctatgc ccgtgtctcg gccgagcaca agctgcgcat cgtccaggcc tggaaacgca
     5041 gaggtgcgat cgtggccatg acgggcgacg gcgtgaacga tgcgcctgcc attaaggctg
     5101 cggacatcgg cgtggcgatg ggtcttgccg gcaccgacgt gacgaaggaa gcgtccgata
     5161 tggtcatcac ggacgataac tttgcctcga ttgccgcggc agtggaagaa gggcgggggg
     5221 tcttcgacaa catccgcaag accgtccatt tccttctgtc atgtaatgcg agcgaagtgc
     5281 tggtcatgct gtttgcgacg ctgctgggcc tgccgcttcc gctcctgccg atccagattc
     5341 tctggatgaa tctcgtgacg gacggcattc cggcgctggc gctggcggtg gatcccaagg
     5401 cgccggatct gatggggcgt cctccccgcc cgcctgatgg ccgattgttg gacggccgac
     5461 gattattcgc gatcggggga gaaggcttga tactggggat gatcgccttg ggggcgttca
     5521 gctacagtct ttacggcctg catcaaaatc tcgaacaggc aaggacggtg gtgttcacgg
     5581 tgatggtcgt cgtgcagctg gtgcatgcgt tcaattgccg gaacgaacgg ctgtcactat
     5641 tccaaatcgg cgtcgggacc aaccgggcgc tcttgggggc cttcctcctc tcgatcgcca
     5701 tgcaggtggc ggtgctcacg gctccacccg tctcgctggt cttcaaggtt gctccgctgc
     5761 cggtcgagga ttgggcgctg atgggagcca tgggcgtgct acccctcgtg atcatagaag
     5821 cgatcaagtt gttgcggcgt acccgtcgtc cgccatccca ttattcacgc ccggtccacc
     5881 actgacaccg aacgaacact cagggtgcag aaacggcgcc cgagttccgc ccgttggcat
     5941 aaaatcgtcg cttcacgatc tcagcggtca ggacatagcc tgccaggatc gctgccaaga
     6001 ggaacaggaa cgacagcggc atcggggtca agcccaatgg ggcaccggtc gggaggagag
     6061 gaagcagcaa tgttacagcg gcgacggcga gggtggccag cgagagatac cgacccggtg
     6121 tgctggtgaa gaatggtcgt cgagtccgaa tgaccagcac aatggctgag gcggaaatga
     6181 cggattccac gaaccagccc gtcctgaatt gctccggcga ggcgtcgagg atccacaaca
     6241 gcgctccgaa cgtcaggtaa tcgaagacag agctgaccaa tccgaacgtc agcataaact
     6301 tccggatgaa ggcaatatcc cagcgtcggg gctggtcgat gagttcatgg tcgacgcggt
     6361 ccgtggcgat cgtcatctcg ggaatgtcgg tgagaagatt cgtcaagaga atctgtttcg
     6421 gcaggagggg gagaaacggt agaaacagtg aggcgccggc catgctgaac atgttgccga
     6481 aattggcgct agttgccatg aagacgtatt ttaacgtgtt ggcaaaggtc ttgcggcctt
     6541 cgcggactcc ttggatcagc accgccagat ccttttcgag cagcacgatg tcggcggcgt
     6601 ccttcgccac gtcgacggcg ctgtcgacag aaatgccgac atctgccgca tgcagtgcgg
     6661 gcgcatcgtt gatcccgtcg ccgatatagc ccactacgtt gcctacatgt ttcagggcta
     6721 ggatgatgcg atctttctga ttgggttcca cctctgcaaa cacatcgatg tcgttgacgc
     6781 gctggaggag ggcttcgtcc gtcatgcgtc gaagatcctg cccggtcaag agccgttgat
     6841 ggtccatccc aacctgttgg ctgacgtgag aggccacgag ccggtgatcg cccgtgatga
     6901 tcttgagcga gacgcccagc cgtcgtaagg acgcgatggt ttctgcgatg tccggcctga
     6961 ccggatcggc gaagaccaag agcccgagga acgtcatctc cgcttcatgc cccttgtcga
     7021 tgtgttcggc ttgacccatg tcgcgagagg ccaggccgag cgttcgaaga ccctgtcggt
     7081 tcaggtcttg aaattgctgt ctgattttct cgctgcggtc ctctatggag accgtcgttc
     7141 cgtccggcag ttcgacgcgg gtgcagacgg ccagcatctg ttccaccgcg cctttggcaa
     7201 tcagaagatg ggtcgttgga gtggccacga gaatggagag ccgtttgcgc aggaagtcgt
     7261 acggcacttc atccagtttc cggtatcccg atagatcaca gatgcactgt gtccggatcg
     7321 cttcatcgag cgggttgata aagccggtct cgtaggtcgc gttgagacag gcatggaaga
     7381 ggacgcgctc gctggggagc ccgtcgagac tcagcgcggc atgcaagcgc atcgtgcctt
     7441 ccgtgagcgt gccggtcttg tccgaacaga gcacgttcat gctgccgaag ttttcaatgg
     7501 aggcgaggcg tttgacgatg accttctgct gggccatgcg ttttgccccg tgtgaaagat
     7561 ttacgctgat gatggccggc aggagttgcg gggtcaggcc caccgcgagc gccatggaaa
     7621 agagaaacga gtcgagcacc gggcgctgga gatagacgtt gatcgcgaag atcgcgaaca
     7681 cgagaaggag cgtcacttcc agcaataagt agccaaagcg ccggacgcca cgttcgaact
     7741 ccgtttccgg aggtcgaagc attacgtgag aggcaatgtg gccgaattcc gtgtccttac
     7801 ccacatgcgc gatgacggcc ttcgcatctc cactgaccac gtgggtgccc atgaagaggc
     7861 tgttggtccg tttgctgagg ggggctgcag cggagacgac cgatgcagtt ttctcggccg
     7921 gataggtttc ccctgtcaat gtggcttcgt cgacaaagag atccttggat tcgagcacgg
     7981 tgccgtctcc gggaatgctg gagccggccg agaggttgat gacatccccc gggacgatct
     8041 cttcgaccgg cacctcgatg agccgcccgt cccgccagac ctccgtcttg acccgaacga
     8101 gtgcgagtaa tgcggcaacc gccttagccg cgccatgctc ctgccaaaac ccaagcagcg
     8161 cgctgatcag aataatggtc aggatgatga gcgcgtcggt ccgatcggcg agaaagagcg
     8221 acacggttgc cgcgaacagg aggatcagga taatcggact ccgaaattgc gagagcaggg
     8281 catacagggg gcgaatatct tggtgtggct tgagccggga agccgcgaag atcgcttggc
     8341 gttgttgggc ctcctcggca tgcagacctt ccggcctcga ctgcagacga gcgaggagtt
     8401 catccgccgg catcgcccaa tagggtcgtt cgttcacaga aatgcttctt tcgatcaggc
     8461 ccggctgtcc gagtccccgg caaccgcacc agccttaggc gagtgataga acgatgtgca
     8521 atcggcttct ctgccacgaa caatccgctt gcctgtgatg ggcgcgcttc gtccagtgac
     8581 caccgtgcat tggcgttcgc cgtggtcatc gtcatagtag aggatgaccc agtcccgagt
     8641 cttcctcagc tggttggcca gggcgctgtt tgagtagagg gcggtatagt gacgagggcc
     8701 tcgctgtgta tgaagaatgg gcagccaggc ttcccgcttg gggttgaacc ggtgcggcgt
     8761 gattttctgc agtgtgccgg ccagagccga ctctcgatat tccctgtcga cgtccagcaa
     8821 ctcctcgatt gatggttctc cggcttggtg acccccgact gtttgaggcg gtctgtgaac
     8881 gcggcccaac cgcgcggtga gtgaatcgat gatcccttga agtttcttcg ggccgactcc
     8941 ttcgatgtcc gccagtcgtc catcgtgtgc cgccatctcg agttgctcca acgtttcaat
     9001 gtgcagatcg tcgtgcagcc ggtgggcgaa cgccgaaccg atccctggga ctgaagtcag
     9061 cagggcgatg ggatccgtat gtccgcgcag gcgatccagc atcggcagcc ggccggttac
     9121 gatcagcgta tggatcgacc gcgccaggct cacgccgata ccgggtaagg cttggagtcc
     9181 atcagttccc tgttgctcga caatccggga gacgggaacc tccaggtgct ccaaccggtc
     9241 tgcggcggtc cggtaggccc ggacacggaa gcgattcgcg ccttgttctt caagaagaag
     9301 cgcgacttcc cgcaactttt gagcgatctc gctgttctgc gtgatagtca tggccttggg
     9361 cggatgagac gcgcgggaag ggcgggacgg gcgcgacgag cgagggttcg aggtccgaag
     9421 ttcgagattt ttggaacttc gaaccccgaa cttcgaaccc cgaacttcga acttcggatc
     9481 gcgcctttct cgcctgtctc gcgcctctcg cactcattca tagtgcagcg cctcgatggg
     9541 attcaacttc gaagctttgt tcgccggata gagtccgaag aacacgccga caacgaggga
     9601 aaagaaaaag gcggcggcca cgacttgcac gttgatgatg gtcggccagc cgatcatggt
     9661 ggtcagcagc cgcgcgctgc cgatgcccgc tccaacgccc aggactccgc cgatggccgt
     9721 catgatgatg gcttcaacga gaaactgcag caggatgtgc gcgcgtttgg cgccgaccgc
     9781 catgcgcaaa ccgatctctt tggtccgttc cgtcaccgat accaaaagaa tattcatgat
     9841 gccgatgccg ccgacgatca gcgagatcga ggcgatgccc atcagcataa acatcatcgt
     9901 ccgactcgtg ccggcgatgg tcttggcgat gtcctccatg gtgcggatgg tgaaatcgtc
     9961 ttcttcggat gggtggacat gatgcctcgc ccgtaggagt tccttaatgt cgtccacgac
    10021 cacagggatg tcatggcgcg tctgggtcgc caccaggatg atcccgacgg tcccaagaaa
    10081 cttcgtgccc agcactttgc gttcggcggt cgtgaagggg aggatgacca gatcgtcctg
    10141 gtcctgccct gtaaccgact gtcccttcga ctccagcacg ccaatcactc gtagtgggac
    10201 gtttctaatg cggatctgac ttcccacgac ttcatcgccg ggctcgtaca ggttttggac
    10261 gaccgttttc cccaacaccg cgactttggc cgctgattcc atgtccgact gggtaaagaa
    10321 gttgccttgg gcgaccggcc agctacgaat gtcggtaaat tggggcgtgg tccccaggac
    10381 gacggtgctc cagttcttgc tttcgccgat gacctgaagg acggagcggg agccatacat
    10441 caccatggtg acgctgccca cttttttctc gatgtcttcg gcgtcatcga cggtcagggt
    10501 cgagatcgtg ccgaggccgc cgcgtacgcc gccgaccgtg gtcgctcccg ggatgatgat
    10561 cagcacattc gtgccgaggc tcgcgatttc cgcctggact gacgccgtcg ctccctggcc
    10621 caggctgacc atcgccacga ccgcgccgat cccgatgatg atcccgagca tggtcaatcc
    10681 ggcccgcaac ggattcctgc ggagaatgcg gagcgcggag agaatggtaa gccagaggaa
    10741 aggcatgatg cgtcgctttc agccttcagc cattcgcctt cagctccgga tcgggagccg
    10801 aatgctgaag gcttgttgct gatagcttcc ttgttctcct catctcatcc tcgggccgcc
    10861 gtcgaagccg ggtggcaatt ttttctgcgc ctgttcttct gctgtttcga tcccgacgat
    10921 caccttgtcg ccttctttaa ccggccctcc gacgacttcg gtaaacaggg aatcggcgat
    10981 gccggtcgtg atcgtggctt gtcgcggagc tgcggcgggg tccagcaccc acacctgtga
    11041 gatcttccgg tccaccggga cgttcggcat ccggaaccgc agcgccccgt tcggtacccg
    11101 caggggatct tctttcttgg ccgtcacaat cgtgacgttg gcagtcatac cgggtttgag
    11161 cttcaattct cgattgtcca ccgtgatcac gacgtcgtaa gtgatcacgt tctgaatgtt
    11221 gatgggcgca ttccgcactt gcgtgaccgt cccctggaag aattgtttcg gataggcgtc
    11281 cacgcgaaac gatgcaggct ttccttcggc caccccgccg atatcggatt cgctcacgtt
    11341 ggagtcgacc tgcatctggg tcaggtcttg cgcgatcaca aagagcgtcg gagtttggaa
    11401 gctggcggcg acagtttggc cgacatcgat gttgcgggag acgacgatgc cattcacggg
    11461 tgaataaatc gtggtgtagc ctaaatccaa ctcggcggat gccagcgtgg cttccgcctg
    11521 gtccagttgg gcctgcgtca cctcgacgtt cgcctcggcg tcacggtagt tggtctcagc
    11581 gagatcgaga tcggcctgcg agacaaaaga ctccggtcgg agaacggcca tgcgatcgcg
    11641 ttcccgcttg cgttgggccg ccaggttctt tgccttggcc agattgccgt tggcgctttt
    11701 cactgcggca cgggcctggc gcacacgcgc ttggaacggt tgctggtcga tgcgggcgag
    11761 gacctgccct ttcgtgacga ccgaattgaa atctgccgag agctccgcga tcttgccgga
    11821 aacctgactg cccaccagga cggagatcac gggattgacg gtgccggtgg cggtgacgac
    11881 ggctgtgatg ggtcctcgat ctaccaaggc ggttttgtac tggacgggag atgaggtcga
    11941 gttccaccag taccagcttg ctacagccat aaaggccagg aagattatga tgctgatgag
    12001 ccagggtaga tgagatcggc tcatccttgg agggagcggg atgactgacg tggggagcct
    12061 gcgcacgtag gcaggtgatg agtcgggatc acgatgtgcc ggcaggtcgg ccgaatgggg
    12121 cggaattact tcggttacgg gaacatggcg ggtgggttcg tcgggattcg tggacatggt
    12181 taatgcactg cttgagtcgg cttgtaagcc ggtttgtgta cggcccggtt tgcgatgatt
    12241 ttacgacctt cttcggcgaa tagcagcacc aaggcaccca atgcgacagg cccgaagatc
    12301 cagaacggga gggagctggt tccgaagatc tcgtgtcccg tcggcgtgta ggtaattagc
    12361 accaggatgg taagctcggt cgcgattccc cacaaaatga gcggattgct gaaccagccg
    12421 agacggtagg cgggcactcg atgagagcgg caggcaaaga cattggcaac ctgcgccatg
    12481 acaatgcctg caaaggttac ggtcgtggcc tccttatata aaggcgaaga ccagctcagc
    12541 ggggttcccc aggtccaccc gttcgcgaag agatagagaa agaatccgcc cattgccact
    12601 atggcttcga tgccgccaag gaagagatag gatcgtagta agagcgggcg attcaggagc
    12661 cgctcagtcc tcggccgcgg cggggtatcc atgacgccgg caagaggacg ttctgtgccg
    12721 aggccaaggg cgggaatcat gtcggttccg agatcgaccg ccaatacttg agggatcgtc
    12781 agagctaacg gcatgccgat gaaaccatag cccagaaacg gaacaatttc agggacgttg
    12841 ctggccagga tatagctggt gaacttgcgg atgttctcgt agaccgcccg gccttcttca
    12901 attgcgctga cgatcgtcgc gaagttatcg tcaagcagaa tcatgtcggc gatctctttg
    12961 gccacatccg tgccggcgat tcccatggcc acgccgatat cggctttttt cagagcgggc
    13021 gcgtcgttca cgccatcgcc ggtgactgca accacctgac ccatctcctt gagtgtggag
    13081 accaccagca gtttgtggcg cggcgccatc cgcgcaaaga tcgggtccgg ctccccggac
    13141 cgagagggtg tcagaagttg acgaagctgt tcttcgctca ttccgtcgag ctgcgatcct
    13201 tcgatcacag ggacgaagcc gattggttgc tgtacggccc cgttcggcac gaggccgatc
    13261 ttacgggcaa tggccagtgc cgtgagtggg tgatcgccgg tgatcatgac gacacgcaca
    13321 ccggctttgc gacacttgac gatcgcatca ggcacctcac ggtgcggcgg gtccatcatg
    13381 gccaccagtc ccaggaacgt gaggtcctct tcgatggttt cgacttcaat gtgttccggc
    13441 tggtgggcaa cctcacgcat ggctatggcg agcacacggt aggcttgctc ggcaaaagac
    13501 cggctctggc tcagaatgcg cgcgcggtcg tcaggagtca tcgtcacggc atccccgtgg
    13561 atctgggctt tggtgcagag ggcaagcacc gattccggag cccccttcgt aaatgcgatg
    13621 agccggcctt cagaccagtg gagtgtgctc atgcgttttc gatcggcgtc gaaggccagc
    13681 tctcccatgc ggcgcagggg tggccgatgg gcgagcccgt gatcgatcgt gaattgcacg
    13741 agcgccactt cggtgggatc gccggtcacg acgaaccgtc cgtcagagcg ccggacgcgt
    13801 ttggcgttat gacagtgcaa gagcgcatcg aagagcgatt gccatcgctc ggcgtcggta
    13861 ttgctgacga tgcggcctct ggtgaagaag cagccttccc gtgattcgat cacgagccca
    13921 tcagcgtaga accgatccac cttcatccga ttttccgtca gggtgccggt cttgtcggtg
    13981 cagatcaccg tggtgcaacc gagggtctcg acggaagaca aatgtttgac caacgcgttg
    14041 cgcttcgcca tgcgctgact gcccatggcc agcgacagag tcactgtggg gaggagtccc
    14101 tctggcacat tggccacgat gatgccgata ccgaaaatgg cgctgatcca gaaaccgagt
    14161 cccgtccata gtccgatgac gaagaacgcc gtgcccatcg ccagcgatat tgccgcaaca
    14221 atccgcgtga ccttgatgat ctctttctgg agtggaccaa ggcttgtctc gaccgtcgtg
    14281 gtgagattgg cgatcttgcc gaactccgaa cgcattccgg tggcgaagac caccgcacgg
    14341 ccgtgcccgg agaggatcgt ggtgccggcg aagacaaggt tcgggagatc aagccagtgt
    14401 ccatcctccg cggcttcggc ggtccgtcgc ttcggtttcg actcgccggt gagggcggcg
    14461 ttgtccaccc gcatgccggt cgcttcgacg aggcgcgcat cggccggcac ccgttcccct
    14521 tcttccagga tgagcaggtc gcctggcacg atctctttcc gtgagatttc gacggattgc
    14581 ccgcttctcg tcacccaggc tttgtccggt agcaggcggc ggagtgcctg tacggcgtgt
    14641 tccgctttgt attcctgcaa aaaggcgaag gcggcattga tgatgatgac acccaggatg
    14701 gcccagccca gcgtggccat cccctcgccg ggtcgcatga agtccgcagc aaatgcgagg
    14761 ccggcggcga tccagagcag cacggcgagg aagtgggtga actgatggac gagcccgcgc
    14821 aggagtgaat agtgatccgg ttctacgaga acgtttggcc cataccgact cagacgttgc
    14881 tttgcctctt cgagagaaag accgccgggg tcggtctcca gctgtttgaa gatggcttcg
    14941 gccggggcct tgtagatagc cggttgagac agctcaggca cgcacacctc gtacgatcag
    15001 gacggagcag ggggcatgtc gcagcaggct ttcggacacg ctcccgagat gaagccgctc
    15061 gcttttcgtc aatccgcgag agccgatgac caggaggtcg gcatggtctg cctcaacctg
    15121 tttgacgatc gtgtcgatca catggtccat gtgcacccgg gtggtgacgg aatagccctc
    15181 tttgatgaaa tcgtctcgca gggcttcgac caggctcgcc gagcgctcca gcaccggttc
    15241 tgtcaactcg gcgagttgcg attcggataa gtaccgtgct gccagatccg tgacggggtt
    15301 ctcagccgag gacacgatcg tgacggcggc ggtttctgga agaatctggg atcggagaaa
    15361 acgcgcggct gcgcgggagt gcttcgagtc gtcgaccgcg agcgtcacgc gccccaatgc
    15421 aggagtcggt cccttgacga cgagcagtga acatggggcc atcgaggcga ctcgccgcga
    15481 gacgcttccc aataagaacc ctttgatatc gctcaagcct cgcgagcccg tgaggataag
    15541 gtccgctttc catcggcggg cctgttgcgc gacggtggat gcgatggggc cataggccaa
    15601 tactttatgg aatttcgtgc ctgggccggt tgcggcctgt ccgagggcga ctttggccga
    15661 tcgctccgct tcgcggagga cgagttcgcc ggccttttcc attgcggcaa tggcacgctt
    15721 ctccgcagcg ggatgtctgt ttttaactga tcgaaggacc ggcttgttga tcacatggag
    15781 gagcgcaaca tgctcaggct ccttccctgc aagcgccgcc agcgcttgga ttccccagcc
    15841 agaaaattct gatccatcga ccgcacaaag aattcgcatg ataggttcct ccctgctcgg
    15901 acaacattct ggatgaggtt caccttacca agttgcattc gatgtgcctg gaaaaggttg
    15961 gattgtgggc tgaaaatcgc cccattccaa ggggttggag agcgtaggga ggagattggt
    16021 ggtggggcgc tgcggcgttt tgcagcagga cagcaattgc tactgtggtg attggcgtca
    16081 gtcgagaggg aatagaacgt cgtgttgaag atcgaacctc gctcggacaa gcgatttcga
    16141 ggtgttattg gctgtgagct gcgtggcact cacattgcga agcctgtcag gaaggagggg
    16201 atggcgatgg gcaaacgatt cattcttgtc atggcgatgt ggcttggcct ccaagccgga
    16261 ttcgtttccg cagaggggaa cactgtcgct cggatcggac aatccggtga ggttttggaa
    16321 ttagaacagc aggtcggcgg gaaggttctt aagaattacc tgcctctcca tgagaaggga
    16381 ggggtgcgat atttcagtgc gggcgtggga ttagaagaac ggtcggcaga gtacccggcg
    16441 ttctcgttga aactggtctt taccgccgga ggaaagccgt ttctcgccgg tgtggatgtg
    16501 gcgatccagc ccgctaaggg cggggcagca cttcgcattc cccgcgagca ggtcgagggg
    16561 ccttggttgt tcgttgatct tccttcaggc atctatgaca tcacggcgac ccatggcgac
    16621 cggacacaag tactcaaggg tgtcaacgtc agcgcgggga agcagagaat agtatatctg
    16681 cgctgggcgg aagatcgtgg cagcgcagga cgtttgccgg cggagtgaat cggactgccg
    16741 tgtgacgatt acagaggggg gaaatgtcat gccgaggaag aagaaagccg gcccagcaaa
    16801 agccaaggac tttgattcca tcaccgtggg ccaggtcatg gagaagcggg tccagttcgc
    16861 ccgcctcaag acgaaaggcg acgtcattgc gtcgctgatg atcgaggggt tcggcgcggt
    16921 gccggttgtc gacggggggc gaagattggc cggtatcgtg agcgaacacg atctgttggc
    16981 ggctatggac gacgggcacg agctgggccg gattacggcg ggagacatta tgactggcaa
    17041 tccgtactcg gtgcggccgg aaacgacagt ggggacgctc gtccacgtct tgcgcgcgag
    17101 cgacctcgtc cgcgtgccgg tggtggatgc caaagacaag ttggtgggta ttattgcgcg
    17161 gcgcgatgtc ctgcgggcct atctcgccgc cggtttatcc aaaagaaagt gagggagatt
    17221 ccatgcggca aaccgaattt ttcatgggcg cctgcgatcc gaaaaccctg acggtcgggc
    17281 agttgatgca ggacgcggtg ttttcttgtg gcccgcgcac gaccgcctcg accctcgcgc
    17341 atctgatgac acaccgcaac ttcggctcgc ttcccatcgc cgaggaagac ggcacgctgg
    17401 tcggcatcgt cagcgaatac gatctgctgc aagcgatgat cgagggccgc gaccttcgca
    17461 agattctcgc cgtcgaggtc atgtcggcgc atcccgtgac ggtgacagaa gagcagacgc
    17521 tcactcaggt agctgatctt ttccaggatc gctacatcac gcgtgttcca gtcgtgcgga
    17581 ataagaaact ggtggggatt cttgccaggc gcgatctcct gttcgggtac ctgaaggctt
    17641 cgcagtactg gtcgtgaaaa gccgtgaggc gtgaggggtt aggcgtgaag cgtggggtgc
    17701 cgcgactgtg atgcggccag gagaatgcgt tgtggttatc acgttaccac ggaatgagtt
    17761 ccgtccgagg tggtgttgct gctctccaga gccatcgttg gtgccctgat gatggcaacc
    17821 tgtctgtccg tttcggttca ggcggatcca gtcaggacac atcgagctcc ttgcgagatt
    17881 acatacccga gcgatgcgac gattgaatgg gagtgccgga cgttacgatc cggcgaatcg
    17941 ctggaaaagt tattcggcga acgctgggtt gatgtcgcac gattcaatcg tatcgatcgg
    18001 cgacatgcac gagcgggcat ttccatcaaa gtcccccagc gcctcgacga gttggtggcc
    18061 tttgctccgc ttcctttctt ctatccaccc gccgagcaag acgagcaatt cattctgatc
    18121 gacctgtcgg aacagttcct tggtgcctat gaacacggta cgctgcgatt tgccctgcct
    18181 atcgtctccg ggaacggaca caaggaaacg ccaaccggcg aatttcgcct gacagccgcg
    18241 caccgcgctc atcaatcctg cctctacacc gttgaaggga cagatcgccc ctatccgatg
    18301 aactacgcgc tacgattcca agtgaacaga gagggcgtgt cctattggat tcatggccgc
    18361 gatctgcctg gctatccgga ctctcacggc tgcatcggcc tctttgatga accgatgcag
    18421 aaggaacagt acgggatccc gaaggatccg gaactgagcg atgcgaagag gttatttgac
    18481 tgggtcttag gagctgagga gcaagacgaa cgcgtgattc ctctcaagca cggaccccgc
    18541 gttcacatca tcggggaagc gccgaagagt ggacgagaat cgccttgatg ataacttaat
    18601 gaagccaatg tggtgccgaa gccgggatga gtttaggggg taagacgccg ttctttggct
    18661 gggatcagct ggtcatctag tctcttgaag tcgcgatatc agaagacgag ggtgacctca
    18721 tcgaatgggt gaccagcttg aaggacgtat cccgcaatcc ttcacacaag ctgattgaat
    18781 tacttagctt gatggttggc ctggagagtc tccgcttttt gccgatggtg gtctgcgagc
    18841 cgctggaggt cgattgcttc cttgcggcac tgttggcccg caatcttgag gccgctccgc
    18901 cggaagtttt taggatcttc gagttgggta attttctgcg ccgaatcttc aaatttcaga
    18961 gccgtaattt ccacctgctc agcctgtttc tgatagatca aggccgctga gagatgatct
    19021 tccgcggctg cggttgattc cagtttgctc tcaagctccg cgggcaggcg ccattcaccc
    19081 actccagggc aaccgagtgc tgactcgacc atcgcgatta cacctgcaaa aataagcagc
    19141 agatttctcc ttgcaagaac aacatgccgt acacgtgtca tagcctttac cctttgcgta
    19201 tgaattgagc gtggaggccg gttagcgaag tggcaagtat gcatgaatat tattccgagg
    19261 ccaataacat cttggcacaa agtgatctcg gtgtgcgcat tgtgcggatc cgctcgctgg
    19321 ccaggctcaa tgaatcagct tgtcactcga gaacgcgcag attctttgtg cgacgttgca
    19381 agaggagagt caacgaggat tattttttct gggcaggcta aagatctccg ttgacactcg
    19441 cgatgagtga cggtcgcgac ctcccgactg accggtcacg tgaactggaa tgcggactta
    19501 aacagaactt tgacgggaga gtacgggccc ggaagacggg ttgtctctca aaatattcgc
    19561 gtccttcgtg cctacgatat cttcatgctc gatcacatcg aatggctgga caaaattgaa
    19621 aaccaaaatg caacaatgcg tacaggattg tacgtataag tattctccac agggctaagt
    19681 cgttgataat gcgacgatgg tcatgctgga tcgcaaattg cttttccctc ctgcaagttc
    19741 gtcttcatca taacgaagga ggaaagaatt atggattgcc cgaagtgcca aggattgatg
    19801 ttgttggaac gtttctcgga tttctttctg gtcttctacg cctggaaatg cctcaattgc
    19861 ggagccatca tcgatcgaac gatctccaat aatcggaaaa acagcctcgc agccaacgcc
    19921 gtgaaagaag ttgaagcggc gactgtgtaa cgctttgacc attggacaca gaagttccgg
    19981 tttaaacagg tccgaattcc gagcatttgt ggaccagaac atcggcgggg cttgcggaca
    20041 acggctgtct gtgacgtgac agcacaagga gcccaccatc acatcctcgg ctccgttatt
    20101 cccctctact ccaacctcaa cataatctcg tgcttcctgg cggatggcgc agtgaaccgt
    20161 gcgctatccg caggtaggcc gacgtaatca ggcttatttc ttcttgctct ttttctctgt
    20221 gcagggcgcc ggacgtttga gttccggcgg tagtttggtc tgctcgtcga gacaggccga
    20281 gtcgagttgt ggctgtgaaa ggcctgtgac cgccgtgagg tttgcgccgc gcaagttggc
    20341 ctgccgcagg tccgcggtat ccaggcgtgc tccggagaga tccgctccat gaaacaatgc
    20401 gccttggagg tttgcttcct ggagattagc gtgcgccagg atggcgtggc tgaaatttgc
    20461 gcctctcaag tctgccgcca cgagattcgc cgcttccaac gtcgcctcgt taaaacgggc
    20521 tcgacgcagc gacactttcg gcgcatagat ttcggtcaag tccgccttcg caaagatggc
    20581 gccttctcct cgtgcctcga tcagcacggc atgatgcagt cgcgcgtctc ggaagtcggc
    20641 ctcgtcgaga tttgcatggt acaggatggc catggtggcg ttcgccgact gagcattgac
    20701 cttgacgaga cgggctccct caaaattgac gccttccagg tcggcctgtt gtaacccggc
    20761 cgactgcaaa ttggtaaagc gcagatcagc gcctcggagc aacgcctcgc gaagattggc
    20821 ttccgtcagg tcggcctcgt cgaaataagc gccttcgaga tccgcttcct ccagccgcgc
    20881 accgctcaac tgagcaccgt cgaggagggc gccttgcaaa ttggtctgat gcatgttcgc
    20941 cttggtcagg ttcgtccggc ggaggtcggc ccccatgaga tcggtaccgg ccagaatggc
    21001 ccgatggaga ttcgctccgt agaaataact gtcgacaaag ctggcgttgg tcaaatcggc
    21061 cgagctgagg tcggctcctt cgaaatgggc ccgttcgaag gtggattcgt gtaattgagt
    21121 tccggtcagg acagcatcga agagtgccgc ctcgtccccg acgctgcgga acagattgac
    21181 ctttgacaat cgagcctttc gcaggtcggc gccggagatg ttgctgtcct ccatgccagc
    21241 tttggtcagg tctgcaccgg cgaggctcgc ctgcgcaagc gtgctctgat acagattggc
    21301 ttgctggagc tgggctcctt ccaaattcgc cttctcaaga ttggcgccga tgagcacggc
    21361 ttggcgaaga tcggcctggc agagatctgc acgcttgtag tccgggttat gccgctcgtc
    21421 cagccacttc ccgtgtgatt gcacgatggc ccggagagtc tttgcgggga tcgtgtgctt
    21481 cttgtgcgcg ctggtgcaag gctttccatg aaacgtcgtc aacccggctt cctcggcctg
    21541 cacggacgcc gtgagactca tggtcaggac tgcggtcaaa atgacatagg cgatcgacaa
    21601 tccgaaagag gcgctacgca tgtatgttct ccttaaaaag tgttcctcgg tgttagcaca
    21661 tctgcaggat gttgaaaagg cccgccagct ttgttctcgc gtcgctcaga ggctcaacgt
    21721 acggcacaga gtacgattcg cctcttcgct cgctgcggtc gtgctgggca ggccttttga
    21781 acatcctacg ggctgtctcg atcctttgag tgacctccaa ctgtactctc tttcgggggg
    21841 tggcaacgta gtttttcaac gacttgctag accgcgcggt ctacgacttg agccaggcgc
    21901 tgcagtgatg gagcgatccg cacctcatac ggcgtcacgg tctcgagcgt tctcattgtt
    21961 tcggggagcc tggtcaattg agtggcagct cgatgacgca gatcagtcag attgggctgg
    22021 tgcgtgaggc gccgcccttc tttcatcacc ggctccaaga gcggttcgcc ctcctgccga
    22081 tcatcgagcg ttgtgacaac atcatgggca agcgttccgt cggatgcgta tcgccgatag
    22141 atctgtttgc ggccgggcca ggtggctttc ccctccgacc gtttgcggca aggcctcccc
    22201 gcgtattcct gcaacttata cgcgcagtcc aaggagggcg catccgctga agtggtcatg
    22261 gcggtgccga cggcgaagct gtcgatcggg gcgccggtgt tcgtcaagtc tctcagcctg
    22321 tattcatcaa gattcccgct ggccaggatc tgagcgtcgc gcaggcctcc ttcatccaga
    22381 atgccgcgaa cgttgcgggc gtgttcggca agatcgccgc tatccaaccg cacgcccttg
    22441 accggtatgc ccatggtcct gagttgcgga gccaggccca cgacgcgccg cgcgccggcc
    22501 tccgtgtcgt aggtatcgat cagcaggacg acattctcgg gttgcgcctg tgcaaaatgc
    22561 tcgaacgcga cgccttcgtc cttgtgggcc tgcacgaacg aatgggccat agtgccgtag
    22621 aggggaatcc cgtattgcat tccggcgagg accgtcgcgg tgccggcaaa accggccagg
    22681 tagcttgcac gcgcggccaa gagtcctgct tcagcgccgt gcgctcgtct caggccgaag
    22741 tcgatgatcg gtttcccccc ggaaaccaag acggaccgtg cggccttcga ggcgatcatg
    22801 gtttgaaagt tcaacagatt catgacgcgc gtttcgagga gttgagccag cgggagaggc
    22861 gcgatgattc ggagaatcgg ttcgttcggg aaaaagattg taccctccgg cagggcgtcg
    22921 acggttccgg tgaaccgcac tccttgcaaa tacttcagca gcgccggtgt gaatcgtccg
    22981 gtcgattcga gccaggccac ttcctcggtt gacacgtaga gctgcgcaag aaattccacg
    23041 acttgttcga ggccggcagc caacaaaaag ttacgatgtg gaggcaactt ccgaacgaaa
    23101 aactcgaaga cagcctcttc ttccatgccc tgttggagat aggcctggat catggtgagc
    23161 tgatacagat cggtgaggag cgcgctggtg gacggattca tacggcgagc gcctctagac
    23221 gaatcggcgc aactcctaat cgcaacattt cctgctcggc attgcggcca tcgtcgggat
    23281 gcacgttcac cgccctgatt ccgtccgtta acaggcagac gtcatacccc aatgcacggg
    23341 cgtctttgac cgtattcaac acgcaatagt cggtggccaa tccgccgata aacaggcgcc
    23401 ggacaccgat tgcttgcagg tggcgatgca atggagtgtt ttgaaacgcg gagtaggctt
    23461 cctgatccgc gtcgatcgct ttataaatga tcacggcgga gaggggagct tcgaatgaac
    23521 gcggcggtaa cgagccggac gatccggcca aacagtgaac cggccagggc cccccgcggt
    23581 ctcggaagga acagtggttg ggagggtgcc aatcgcgtgt cagaaaaatc ggaagcgatt
    23641 tcgagtggaa gcgatggata taggtcagca gaactggaat gatctcgttg ccgccgctga
    23701 tgcccagtgc gccgcccggc agaaagtcgt tctgtatgtc cgcgaccagc aaggcgtcat
    23761 gggcgtcgag cgacttcatt atggatgagt tctcctgtct gattcatccc ggcaagcggt
    23821 ctcaccgtcc ctgttgagac agcaaccgga tgtacgcgac gacgtcctgc atttctccgt
    23881 ccgtgagctg gccccgccag gagtgcatgg ggctgaagac gacgccgtgc tcaatcgtgc
    23941 gcaacagctc ctcatccgat ttgagaaatg agttgaaccg gtgaaaattg gccggcgcga
    24001 ctttcagtga cgcggcattc gggccgtcgc cccatccgcc cgtgccgtga caagcgaggc
    24061 agtggcgttc gtacacggct ttccctcgcg tgatgtcagc ggggtagtcc tgtgcgagaa
    24121 gcgccgtcgg cacggcgagt gccccgcaca ggagaaagcc acagaacagt gacgcgcgca
    24181 tagttctcct caacttttct tcgtcgtgtg agcgatgaac gacgtataga gctgccgcat
    24241 gttcgtactc ttacaggatg gacaagccac ttttcccgtt tcgtgctctc tcaaggtgag
    24301 gacgatcaat gattcttttc cgcagtccag gcacttatag tcgtacatcg gcatcgtgca
    24361 cctcgcagcg ttcagctttc cgtgatgatc cgcccctccg acttcatttc accctcgccc
    24421 cactcggcga cagcacgggc catttcttcc agccgtgcgg ccgcgcgccc gtatacggta
    24481 tccggaggat acagactgtc cagtccgcgt tctccggcgg ggaggccggt taataactcg
    24541 atggcttcct ccaccgtgcc gacggcatag acggcgaact gccccgcttc aactgcttcg
    24601 acgacatctc ggcgcagggc caaatgcttg ctgttgcggg agggaatgat gaccccttct
    24661 ttgccggtca atccgcgttt cttgcaggat tcaaagaagc cttcgatctt ctcgttgacg
    24721 cctccgatgg gctgcacttc accgagctga ttggctgagc ccgtgaccgc caaccactga
    24781 cggacgggca ggtcagcgag actggacagt atcgcgacaa gctcggccac ggtggcgctg
    24841 tctccctcaa tttccgaata tgtctgttca aaggtcaacg tcgcgctcag agcgaaagga
    24901 tgggctccgg cgaatttgcc cgccagaaac ccgccgagcg tcatgactcc tttgctatga
    24961 at
//
LOCUS       DATIFR010000066         1886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_807660, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000066 DATIFR010000000
VERSION     DATIFR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807660"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VLD60_03780"
     CDS             complement(<1..720)
                     /locus_tag="VLD60_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44104.1"
                     /translation="MNCRGTGLRNAVVLFLGLAILGCSDISQDGAPTGRVLGSNSGVG
                     KGTVSSYAEFDKNGAPKAIGIVFQAGALDGLPSAHSDGHHCFDRNNDGKVELAKECFG
                     THEWVIPLPSEAARRSEIPFKWVGLNWNPYGHIPPGVYDLPHFDVHFYIEPIEKIFAI
                     GSGPCGPEFVRCDQFKVATRPLPSNYMHSDFKNVDAVAPAMGNHMMDLTSPEFDKKKF
                     TQTWIYGAYDGRITFYEEMLTR"
     gene            complement(849..1793)
                     /locus_tag="VLD60_03785"
     CDS             complement(849..1793)
                     /locus_tag="VLD60_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HSB44105.1"
                     /translation="MTDKKIIAVVGSTGSQGGGLVHAILNDPNGGFAARAITRDPNKD
                     KAKALAAKGAEVVKADLDDVDSLKKAFAGVYAVYGVTNFWEHFSAEKEKAQAKNIADA
                     AKAARVKHIIWSTLEDTRKLMQPDDKRMPILQDKYRVPHFDAKAEANASFAGLPVTYL
                     VTSFYWDNLYMFGFAPKKGDDGVYSWTFPMGNAKLAGMAAEDIGKTAYGIFKAGDRYI
                     GKTVGIVSEDLTIAEMGDKLTKGLGIGPVTYNAVDANMYRGFGFPGAEEMGNMFQVYR
                     DFEKEVLSARSVETTRSLNPQLQSFDQWLAKNKSSVPV"
BASE COUNT          369 a          544 c          509 g          464 t
ORIGIN      
        1 gcgcgtcaac atctcttcat agaaggtaat gcggccgtca taggcaccgt agatccaggt
       61 ttgcgtgaac ttcttcttgt cgaattcggg gcttgtcagg tccatcatat gattgcccat
      121 cgccggagcc accgcgtcca cattcttgaa gtcggagtgc atgtaattgg acggcagcgg
      181 cctggtcgcg accttgaact ggtcgcagcg gacaaattca ggaccgcagg gaccagagcc
      241 gatcgcaaag atcttctcga tcggctcgat gtaaaagtga acgtcgaaat gcggcaggtc
      301 atacacccca ggagggatgt gtccgtacgg gttccagttc agaccgaccc atttgaacgg
      361 aatctccgaa cgtcgcgccg cctcgctggg cagcgggatg acccactcgt gcgtgccgaa
      421 acactctttg gccagttcaa ccttcccatc gttgttccgg tcaaagcagt gatgtccgtc
      481 ggaatgtgcg ctgggaagcc cgtccaatgc gcccgcctga aaaacgatgc cgattgcttt
      541 gggggcgccg ttcttgtcga actcggcgta gctgctcacc gttcctttac cgacgccgga
      601 attgctgccg agtacacgcc cggttggcgc gccgtcctgg ctgatatcag aacatcccag
      661 gattgccaga ccaaggaaca acaccactgc gtttcgtaat ccggtgcctc gacaattcat
      721 ggtcttcccc ctttactgtt gaagcatttc attaagtgtt ggttgactca ttgttgccgg
      781 tggcgaaatg ccacttcggg ggaaagacct gcgcgtcggt catggtgtgt tttgagacgg
      841 ccaagcgatt agaccggaac gctgctctta ttcttcgcga gccactggtc gaaactttga
      901 agctgcggat tgagcgaccg agtcgtttca acgcttcgtg cactcaggac ttctttttca
      961 aagtctcgat acacctggaa catgttgccc atttcctcgg cgccggggaa cccgaagccg
     1021 cgatacatgt tggcatcgac agcattgtat gtgaccgggc cgatgccgag acctttagta
     1081 agtttgtcac ccatttcggc gattgtcagg tcttcactca cgataccaac cgtcttgccg
     1141 atgtaccggt cgccggcttt gaaaattcca taagccgtct taccgatgtc ttctgctgcc
     1201 ataccagcca gcttggcgtt gcccatcgga aacgtccagc tatacacgcc atcgtcgccc
     1261 tttttcggtg cgaagccgaa catgtagagg ttgtcccagt agaacgacgt cacgagatac
     1321 gtgacgggaa gccctgcaaa ggaagcattt gcttctgcct ttgcgtcaaa gtgcggaacc
     1381 cggtatttgt cctgcaggat cggcatccgc ttatcgtcgg gctgcatcag tttccgcgtg
     1441 tcttcaagtg tggaccagat gatgtgcttc acgcgggccg ccttcgccgc gtcggcgatg
     1501 ttcttggcct gagccttttc tttctcggca gagaagtgct cccagaagtt ggtcacgccg
     1561 tagacggcgt agacgccggc gaatgccttc ttcaggctgt cgacgtcgtc gagatcggcc
     1621 ttcaccactt cagcgccttt ggcggcgagg gccttcgcct tatctttatt tggatcgcgg
     1681 gtgatcgcgc gtgctgcgaa acccccgttc ggatcattca ggattgcgtg aaccaatcca
     1741 ccgccctgtg aaccagtcga accgactact gcaatgattt tcttgtcagt cacgtctatc
     1801 tccttggtaa gtagttaccc attagttctt tgcgacgatt gccgcgcctt cggcaagcgc
     1861 tctcagcctg gagtactcct agtccc
//
LOCUS       DATIFR010000067         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_617652, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000067 DATIFR010000000
VERSION     DATIFR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617652"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VLD60_03790"
     CDS             <1..772
                     /locus_tag="VLD60_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HSB44106.1"
                     /translation="FYGMRLIQFPLGIFGVALAMAILPTLSAQAARGALEELRTTLGF
                     GLRMIVFIMLPAMLGLIFLRTPIVHLFFEHGTFTAQDTAETAMAVLCYAVGLWAFGGV
                     RIIVAAFYSLQDTKTPAISAAIAVTANIAFSLLLMSPLGASGLALATALAAMVNGGIL
                     VAVLNRRLGGVDWGSVGRSALRVFVACVPLVAACLWVSSAQVWTHADEWTAKSVMLAV
                     AIGLSVSGYLGVHALLRSEELDLVWGMVRRKLGRAMGK"
     gene            781..1311
                     /locus_tag="VLD60_03795"
     CDS             781..1311
                     /locus_tag="VLD60_03795"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSB44107.1"
                     /translation="MRRTMIFKSRWGWMGVVETENGIATIVLPKASRMMVAADVRGGS
                     RGQSEEGSSPGLRAARKQLTEYLAGRRTSFTLPLDLSAGTAFQRRVWRALQNISYGYL
                     HSYQELAALVGGPQYARAVGNAVGANPLPIVIPCHRIVSQDGSLGGFSCGLPAKRRLL
                     ALEGSLSRICRRGHNS"
     gene            1308..1769
                     /gene="dtd"
                     /locus_tag="VLD60_03800"
     CDS             1308..1769
                     /gene="dtd"
                     /locus_tag="VLD60_03800"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSB44108.1"
                     /translation="MKAVIQRVTRASVEVDGCVVGKIGEGLLVLLGVAKGDGEADCQF
                     LVEKIRTVRIFSDEAGKMNRSLTDVKGSVLLVSQFTLLGNTRNGRRPSFDEAAPPEEA
                     KRWYDATGALLRAQGIPVETGIFAAHMKVELLNDGPVTFVLDSRSGQSIAS"
     gene            1823..>2032
                     /locus_tag="VLD60_03805"
     CDS             1823..>2032
                     /locus_tag="VLD60_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44109.1"
                     /translation="MGTTVPARHPLRQLFGSLTEKSFTEHLGWPDLNVTSYVSNLLVD
                     FTRTDQLYKIRNRQDKPVETVVDLLF"
BASE COUNT          367 a          527 c          669 g          469 t
ORIGIN      
        1 gttctatggc atgcggctga ttcaatttcc cttaggcatc ttcggcgtgg cgctggcgat
       61 ggccatcctg ccgacgttgt cggcgcaggc ggcgcgcggg gcgctggagg aactccggac
      121 gacgctcgga ttcggtttgc gtatgattgt gttcatcatg ttgccggcga tgctgggcct
      181 gatttttctc cgcacaccga tcgtccatct gttttttgag catggcactt tcacggcaca
      241 ggatacagcc gagaccgcca tggcggtgct gtgttatgcc gtgggcctgt gggcgttcgg
      301 cggcgtgcgg atcatcgtgg cggcgttcta ttcgttgcag gacacgaaga cgcctgccat
      361 ttccgccgcc attgcggtga cggcgaacat cgcgttttcg ttgctgctga tgtcgccgtt
      421 gggcgcgtcg ggcttggcct tagcgaccgc gttggccgct atggtgaatg gcggtatcct
      481 ggtggcggtg ctcaatcgcc gtctgggtgg cgtggattgg ggatcggtgg gacgttctgc
      541 attgcgtgta tttgtggcct gtgtgccgtt ggtcgcggcc tgcctgtggg tctcaagtgc
      601 tcaggtctgg acgcacgcgg acgaatggac ggccaagtcg gtgatgctgg ctgtggcgat
      661 cggtctcagt gtgagcgggt acctgggcgt acatgccttg cttcgttcgg aagagctgga
      721 tctcgtctgg ggaatggtca gacgcaaact cggtcgcgcc atggggaagt aggagacagc
      781 atgcgccgca cgatgatctt taagtcgcga tggggctgga tgggggtggt tgaaacggag
      841 aacggcatcg cgacgatcgt cttgccgaaa gcttcgagga tgatggtggc ggctgatgtg
      901 cgaggcggtt ctcgtggaca gagtgaagag gggtcgtcac ctggactgcg cgcggcacgg
      961 aaacaactga ccgagtatct agcgggaagg cgcacctcat ttacgcttcc cctcgacctc
     1021 tcggcgggga cggcatttca acgacgtgta tggagagcac ttcaaaacat ctcttatggc
     1081 tacctccatt cctatcaaga attggccgct ctggtcgggg gccctcagta cgcaagagct
     1141 gtcggcaacg ccgtcggcgc caatcccttg ccgattgtca taccctgtca tcgaatcgtt
     1201 tcacaggatg gttcgcttgg cggtttctct tgcggattgc cggcgaaacg gcggctgctt
     1261 gcccttgagg gttcgctctc cagaatttgc cggagaggtc acaattcatg aaagcggtga
     1321 tccagcgcgt gacgcgagcc tccgttgagg tggatggttg cgtagtcgga aagatcggag
     1381 aagggttgct agtcttgctc ggcgtcgcca aaggcgatgg ggaagcggac tgccaattcc
     1441 tcgttgagaa gatccgcacc gtccgaatct tctccgatga agcggggaaa atgaatcggt
     1501 cgttgacgga cgtcaaaggg tcggtcttgc tcgtctccca gtttacactg ctggggaata
     1561 cgagaaatgg tcggcgcccg agttttgacg aggccgctcc gcctgaggag gcgaagcgat
     1621 ggtatgacgc aacgggagcc ctcctgaggg cacaaggaat cccggttgag accggcatct
     1681 tcgccgccca tatgaaggtc gagttgctca acgatggacc ggtcaccttt gtgctggata
     1741 gtcgcagcgg gcagtccatc gcgtcctgaa gagagtctgc tatactatgg tttgatctct
     1801 gtgcatccag tgcgagggag agatgggaac aacggtcccg gctcgacatc cgctgcgaca
     1861 actcttcggt agcctgaccg agaagagctt taccgaacat ctcggctggc ccgatcttaa
     1921 tgtgaccagt tacgtctcaa acctcttggt cgactttacc cgcaccgatc aactctataa
     1981 gatcagaaat cggcaggaca aaccggttga gacggtggtg gacttgctgt tc
//
LOCUS       DATIFR010000068        14472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2726012, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000068 DATIFR010000000
VERSION     DATIFR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726012"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="tolB"
                     /locus_tag="VLD60_03810"
     CDS             <1..1323
                     /gene="tolB"
                     /locus_tag="VLD60_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HSB44110.1"
                     /translation="SLVMGGAVALAGAIGILESGAADVFLEATRPDFQKIPLGVLGIE
                     NGGGPDWLGGRLEEVLKKDVKRSLVFELMDLPSLGIKVHDLGNGLGNGSKAVFKQAAE
                     NGVSVLVWGKAGLKKGEKEDDVNMEGFVYDSGSDEVVGGKRYAGSPSVVRLMAHRFAD
                     ELVFRYTGEPGIARTKIAYVSAQGSARELFVMDYDGYDARQLTADGFLNLMPRWSPDR
                     RFLVFTAYRDRNTQDIDMIELATGKRWTLVSFGGLNITPTLSPDGNFLAFASSYEGNS
                     EIYRLDTRTKAIQRLTVNPAGELSPSWSPTGRELAFVSDRGGGPQVFLMSADGSNVRR
                     LTFEGDYNAAPAWSPRGNWIAYVCRTPRKEYKLCAISPDGQKRMQLTTGGGVDDSPSW
                     SPDGRHLTFSSTADGKSHIYMIDADGKNLERLTFDGTHNSAPTWSPAS"
     gene            1392..2072
                     /gene="pal"
                     /locus_tag="VLD60_03815"
     CDS             1392..2072
                     /gene="pal"
                     /locus_tag="VLD60_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSB44111.1"
                     /translation="MRTSTMKIAVALVATFALAGVWGCSKKAVQSGGDTQSSQQGAAK
                     SGTPDSSQSGVGSFPDTSMQSGGGSGLRGLDKNPSEERIGSGTVLAKADTSGSANRQL
                     DEMRAEQAASAAAGLRDVFFGYDSWTIGEDGRQALSRDAEWMKTNLGAAVKVEGHCDE
                     RGTSAYNLVLGEKRAKAVRNYLVELGVSANRLSVVSYGKERPFCNEHAESCYQQNRRG
                     HLVVKSGK"
     gene            2127..3647
                     /gene="ybgF"
                     /locus_tag="VLD60_03820"
     CDS             2127..3647
                     /gene="ybgF"
                     /locus_tag="VLD60_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSB44112.1"
                     /translation="MSSVGGVMQKAWNLRSIGTLFCLLSAGLMSGCVAQQADLKSAER
                     RFKESNEALAKRGAQQRQELEELRGEELPKLRGELERAQHQAQEIQKAQDDLRQRSAV
                     LEQQTKKLEQLSGKLEAESTNRYNQVRESLNAQDVKNKADRDQLRMDVNNRLDEINRQ
                     MDLLRKDIIEAVQKTNSGLVKSVDAKLDEQRKTLADNQARTDQLSGKFTQFSQALTGF
                     RESLTTLGERVGQEEQANKALAAKVEADTKASTAHINETTKAMSGHLGEVNKSVTAVA
                     QKLATRLDEQDHRLEALSKAVDQVAQDGHTRGGNNKAQTRQQPTKSTQRSSLAPAPEP
                     VSQHDPEPVTVSAQEPEHQTQAVEPAGGTGESPEPASPAVARAAERSDKVEYERLLAF
                     FRNGNLEEARQGFSTFLTEYPNSDLAPNARYWLGESHYGKKDYKQAIDSYDRVELDYP
                     QSEKVPAAILKKGYAYLALKDKKRASSAFKQVVTLYPKSPEAGKAYDKLSQLKDSR"
     gene            3644..4426
                     /gene="ybgF"
                     /locus_tag="VLD60_03825"
     CDS             3644..4426
                     /gene="ybgF"
                     /locus_tag="VLD60_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSB44113.1"
                     /translation="MIVTQAIVRVIGAGIVVWMAVLVAGCAKHADFIELRDHLATVSK
                     SQDQDQKRLDALQRRMESLERIKDAEPTKTKLDEILARVQKLEARVAKLDEGTGVAAV
                     PKGEPLPIDPARPGKPQKPGGSPEGGSIVPNVPAITPTSAFNLSYNDYLNGKYDLAVA
                     GFQRFVKDFPGTSLTPNAYYWLGESYYQQKDYVRAMQSFEYVATEFPGNEKVPAALYK
                     LGLAAAETGDLVKSKKNLKRVLEEFPTSDEAKLAKNKLAEIR"
     gene            4490..6283
                     /gene="mutL"
                     /locus_tag="VLD60_03830"
     CDS             4490..6283
                     /gene="mutL"
                     /locus_tag="VLD60_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSB44114.1"
                     /translation="MPAASDVTTIRVLPGEVVSRIAAGEVIERPAAVVKELIENSLDA
                     GSRHISIDVKDGGVALIRVTDDGEGIRRADLPLAFERHATSKLKSDEDLASIRTMGFR
                     GEALPSIASISKVQVTTVTRREKLGAQFTILGGMSQGIADAPPMPGTKIEVSDLFYNQ
                     PARKKFLKSTVTEFSHISNVVQQAGLGWPSVHFRLTHNGHEIMNYPAVASDRDRILQV
                     YRSAFLDKAIDLRGRMAGCAISGVIIDPVHARSSRVPQDLFVNRRPIRNATVFHAVTD
                     GFGSFLPKGHHPTFVLFLDIDPERIDVNVHPAKREIRFADQEAIHQFVRHAVRHALSG
                     PERKVVLTVGGSEALPRADSPSVHPARRVQDRPVPSQSAQGAHDDPAVVTGSQSLFTH
                     EAMASYADSSAPAVVPLGQMQRTYLVAQVGQELQVIDQHTAHERVLFQRLWKKWLNRE
                     IATQPLLIPEPIELAAAQSMRLQKHLGDLEQLGLIIEPFGSGAVALRAVPAGLGQMNG
                     GLLVQDLLEDLTEWDSASSLEARVQPLLASLACRSAVQAGRSMALPEIEQLVRDWVEE
                     GLIMTCPHGRRTAFRLSTDELDKLFGRVGWS"
     gene            6280..7317
                     /gene="miaA"
                     /locus_tag="VLD60_03835"
     CDS             6280..7317
                     /gene="miaA"
                     /locus_tag="VLD60_03835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSB44115.1"
                     /translation="MTTIPILTMYPLSETLIHLKPLVVIVGPTAVGKSRIAIEVAKRF
                     ETEIVTADSRQVYRGMDIGTDKPHEESRQGIPHHLIDLVNPDHSFNAGLFRRHAVETI
                     ERLYQRRQLPLVVGGTGLYVRTLLQGLCEAPPADPAVRAQLHEEARTQGHDRFYARLV
                     AVDPVTASKLHPRDTSKVIRALEVHQLSGRAMSEFQQRHGFSERPYSPLVIGLNRDRG
                     ALYRRIEERIDWQLAHGLLQETQRLLDQGYKRNCTAMKGLGYRQVAAHLAGEYDQAEM
                     VRRFKRDTRHFAKRQMTWFRREPAISWLMVSESEATAETVDRVIHRVEQFLAGLDGVD
                     AGQRSVEENRR"
     gene            7325..8224
                     /gene="mtnP"
                     /locus_tag="VLD60_03840"
     CDS             7325..8224
                     /gene="mtnP"
                     /locus_tag="VLD60_03840"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HSB44116.1"
                     /translation="MGKRIPASRAAIGIIGGSGLYDIEGLRHVKHVPVRTPFGQPSDD
                     IVLGELEGVRVAFLSRHGRGHRLNPSDINYRANVYAMKSIGVRRIISVSAVGSMKESM
                     KPGDVVLPDQFIDLTKRRISTFFEGGAVAHVAFGEPVCASLGASLLAAARSTGATVHQ
                     GGTYICIEGPQFSTKAESNLYRQWGVSVIGMTNLPEAKLAREAELCYATVALVTDYDC
                     WHETEEAVTVEAILATLRQNVALAKRLLRGAVASAASVESCGCHGALQNALVTDPNRI
                     PAALRRKLAPLTERVLSPKKGTR"
     gene            8229..9143
                     /locus_tag="VLD60_03845"
     CDS             8229..9143
                     /locus_tag="VLD60_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB44117.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEAAEVLGGSATYFSTSASYFTAVD
                     LIAVVGDDFPSQHLTFLKSRGIDLTGLERRSGATFRWKGEYTHQLNEAHTLDTKLNVF
                     ETFRPKIPESYRSPEVLFLGNIDPELQLDVLHKLPRPPLVACDTMNFWINGKRDALWR
                     VLEQVDILIINDGEARALGGDSNLVKVAQKVLARGPKHLIIKRGEYGVLMFNEKQVFG
                     APAFPLEDVRDPTGAGDTFAGGFLGHLAATGNRNVESFKQAIVFGSVMASFTVESFSL
                     DRLRVLDYKEIQERFRAFKRLTHFEDLP"
     gene            9140..9820
                     /locus_tag="VLD60_03850"
     CDS             9140..9820
                     /locus_tag="VLD60_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44118.1"
                     /translation="MTFIPARFPLRRCPILAVVVAVGLLSGCATSEEAKQRSRGYYQE
                     GMASIDTDRQKAFVSFQKAVQIDPDNKEARYGLGHILVYQGKLSQAEREFREAIRIDE
                     DYSEAHTYLGQVLASQDRWPEAIAEFRKALNNPLYPTPDLARFHLGRALAHEGDLQGA
                     MEALEDAQAVNPSSVPPALLQLELGRVYYKMGFERRAKDALTKVTKMDNSGEYATAAK
                     EILARLKP"
     gene            9831..10769
                     /locus_tag="VLD60_03855"
     CDS             9831..10769
                     /locus_tag="VLD60_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HSB44119.1"
                     /translation="MESIGDFFKQVRETKGLTVDEVASKTRIRSDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFIQSAGAFYEKQDERERLKARQAEEERRRQA
                     NRKAVAIAIGIAILTLVVLLSREQSSILVRRASTETPPASTKRTVTPESGSHDVPSRP
                     EGEGASSTLKPKSSETPSSSTASRGDTSPTPHGTAAPSVGASAQQQTALPVPPPISTD
                     SPLGGTSPESSPNAEEKLVLDLEATELSWVVIQIDGGSPQEALLRPGEKARWKGQDQF
                     ILTLGNAGGVKAELNGKPQKPFGPSGKVARDILLKR"
     gene            11253..11702
                     /locus_tag="VLD60_03860"
     CDS             11253..11702
                     /locus_tag="VLD60_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44120.1"
                     /translation="MGIFSKFLGISAAVLMLSATVVGAEEKDPLKPRVPADQIADAKA
                     MKNPVAATPESIAKGKALFEGKGTCFNCHGKEGKGDGPAGAILNPSPRNFTNCKFHKK
                     RKDGELFWVIKNGSAGTGMVSLIPAAINEEEAWTIINYERSFCKGEE"
     gene            complement(11912..13624)
                     /locus_tag="VLD60_03865"
     CDS             complement(11912..13624)
                     /locus_tag="VLD60_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSB44121.1"
                     /translation="MQYQNGCASLRILDVGPGIESVLTDAAAKNFQAVTVTGGAPGPI
                     PPDTEILVTLQRSGLKLWQDNLYDRVPADITFEALLTFKDAAGKELGQQTITIAHNER
                     LILEPTQKRCDYGNVDEFVYDAGVKLSVQFILAARSQLASAGSAPLASAGSTPSEKVA
                     ASSSPGRPAPPPAAVEAKGAPSTLSFKATMLDENGNLVFESGERIRVRVDLVNAGSQE
                     LQNVTATITGSPSLLAQFPATTLSAGRLQPGQSRSLEFVATLPQGPQQQKAELLVSVF
                     DPAAGAPPSQALSLSIQPTGVTADDVDQVPAAAAGFKRPHTYLLSIGVGSYRDQHLSI
                     RKYAASDAEILTSYFQSLGGLPASNVRLLQDWKALRPDIDEALLDWLPPHMNKEAVVI
                     VYFAGLATVSSSGEVFLLPYDGNATNTSRAYPLKDLEAALARLKAKHTILLFDGMVAR
                     LGGESRAKSTVPQWSPAGNSTLHIIASNGIGRIIEDDKHRHGLFTYYFLRAMRGEADS
                     NHDGEVTLGEIVLYLTQKVLWASKTQYGQEQRPLVLPQFRNGDPAASFPLSRLATAQA
                     RETP"
     gene            13993..>14472
                     /locus_tag="VLD60_03870"
     CDS             13993..>14472
                     /locus_tag="VLD60_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HSB44122.1"
                     /translation="MLTLGLSNMRDAAAALVDNGRVIAAAEEERFVRVKHVTALPVHA
                     IRFCLQAAGIRLCDIDAVAVPWKFWQLGRRASLAVGSMLRSPQLFRVKGIRSVERLAH
                     EWKELAFLRWYLSRLIDGTASPPPVFLDHHLCHAASTVLVSPFDRAAVLIADGASEAH
                     "
BASE COUNT         3179 a         3760 c         4477 g         3056 t
ORIGIN      
        1 agcttggtga tgggtggggc ggtggctctt gccggcgcga tcggcattct ggaatctggc
       61 gcggcagacg tatttcttga agcgacacga cccgacttcc aaaagattcc actcggcgtg
      121 ctgggcatcg aaaatggcgg aggtcccgac tggctcggcg ggcgtctcga agaagtgctg
      181 aaaaaagacg tgaagcgttc cctggtgttc gagcttatgg atcttccgag cttggggatc
      241 aaagttcacg atttggggaa tggcttgggg aacggatcga aggccgtctt caagcaagcc
      301 gcggaaaatg gggtgtcggt tttagtgtgg ggcaaggcag gtttgaagaa aggggagaaa
      361 gaagacgacg ttaatatgga aggcttcgtg tatgacagcg ggagcgacga agtcgtcggt
      421 ggaaaacgct acgcgggatc cccctctgtc gtccgcttga tggcgcatcg ctttgccgat
      481 gaactcgtgt ttcgctatac cggagagcct ggcatcgcaa gaaccaagat tgcatacgtg
      541 tccgctcagg gatcggcccg agagctgttc gtcatggatt atgatgggta cgacgcgcgg
      601 caactcaccg ctgatggatt cttgaatttg atgccccgct ggtctcccga tcgtcggttt
      661 ctggtattta ctgcctatcg tgatcggaac acccaagaca tcgacatgat cgaactcgcg
      721 acggggaagc gctggacgct ggtttcattc ggcgggctga acatcacgcc gaccctctct
      781 ccggatggaa actttctggc atttgcgtcc agttacgaag gcaactccga gatctatcgg
      841 ctggatacga ggaccaaagc cattcagcga ctaaccgtca atcctgcggg ggaactctcg
      901 ccatcctggt ctccgactgg tagagaactt gcttttgtct ccgatcgtgg gggtggacct
      961 caggtgttct tgatgagcgc ggacggctcc aatgttcgcc gtttgacgtt cgagggggac
     1021 tataacgccg cccctgcctg gtctcccagg gggaattgga ttgcctatgt ctgccggact
     1081 ccccgcaagg aatataagct ctgcgcgatc agcccagacg ggcaaaagcg tatgcaattg
     1141 actacagggg gtggcgttga tgattctcct tcctggtcac cggacgggag gcaccttacc
     1201 ttcagctcga ccgctgatgg gaagagccac atttacatga tcgatgccga tggaaaaaat
     1261 cttgagcgcc tcacgttcga cgggactcac aatagcgcgc cgacctggtc cccggcatct
     1321 tagtccatgg actccctccg agggagtttc acaacatcac gatgaataac tttgagagag
     1381 gagaggcatg tatgcgcaca tcaaccatga aaatcgcggt ggcgctggtt gctactttcg
     1441 cgcttgcggg agtttggggc tgttcgaaga aagccgttca atcggggggt gacacacaat
     1501 catcgcagca aggtgctgcc aagagcggga ccccagacag ttcgcagtcc ggcgtcggaa
     1561 gctttccaga cacgagcatg caatcgggag ggggctccgg cctcagagga cttgataaga
     1621 acccctccga ggagcgtatc ggcagcggaa cggtgctcgc aaaagccgat acctccggca
     1681 gtgcaaaccg gcagctcgat gagatgcgcg cggagcaggc cgcttctgcc gccgctggat
     1741 tacgtgatgt gttttttggc tatgacagtt ggacgattgg tgaagacggg cgacaggcgt
     1801 tgagccgcga tgccgagtgg atgaaaacga atctcggcgc ggcggtcaaa gtcgagggcc
     1861 attgcgacga gcgcgggacc tccgcctata acctggtgct gggtgaaaag cgtgcgaagg
     1921 ccgtgcgcaa ttatctcgtc gaactcggtg tgagtgccaa tcgactgtcc gtggtgtcgt
     1981 atgggaaaga gcgtccgttt tgcaacgagc atgcggaatc ctgctatcag cagaacaggc
     2041 gcggacatct tgtcgtcaag tcaggcaagt aataaggcgt ccttgccgcg tctaggatcc
     2101 ggcgagggcg gaagaggaac atggtcatgt ctagcgtagg tggcgttatg caaaaggctt
     2161 ggaatctgcg aagcatcggc acccttttct gcctgctctc cgccggcttg atgagcggtt
     2221 gtgtcgctca acaggccgat ttgaaaagcg cagagcgtcg gtttaaggag tcgaacgagg
     2281 ctctggccaa gcgaggtgcc caacagcgac aagagctcga agaactgagg ggagaggagc
     2341 ttcccaaatt acgcggcgaa ctcgagcggg cgcagcacca ggcgcaggag attcaaaagg
     2401 cccaggacga tctcaggcag cggtcagcgg tcctggaaca acagacaaaa aagttggaac
     2461 aattgtccgg aaagctggag gctgagagca cgaatcgtta taaccaggtg cgcgaaagtc
     2521 tcaacgcgca ggacgtcaag aacaaggcgg atcgggatca gctccgtatg gacgtcaata
     2581 atcgtctcga tgagatcaat cgacaaatgg atctgctgcg caaagacatc atcgaggccg
     2641 tgcaaaaaac taacagcgga ctggtgaaga gcgtcgacgc caagttggac gagcagcgaa
     2701 aaacgctggc ggacaatcaa gccaggaccg atcagctctc tgggaagttc acgcaattca
     2761 gccaagcttt gacgggattt cgggagtcgt tgacgacgct cggtgagcgg gttgggcaag
     2821 aggagcaggc aaacaaggcg ttagccgcca aggttgaggc tgataccaaa gcctcgacag
     2881 cccatattaa tgagacgacc aaggcgatgt ccggtcattt aggcgaagtc aataagagtg
     2941 taacggccgt tgcccaaaag ttggcgactc gtctcgatga gcaggatcac cggctcgagg
     3001 cattgagcaa ggccgtcgat caagttgccc aggacggtca tactcgtggt ggcaacaata
     3061 aagcccaaac ccgtcagcag ccgacaaaat caactcagcg ttcttcgctt gcccctgcac
     3121 cggagccggt ttcgcaacat gatccggaac cggtcacggt atccgcgcag gagccggagc
     3181 atcagactca agccgtggaa ccagcggggg gtacagggga gtctccggaa ccggcatcac
     3241 cggcagttgc ccgtgcggct gaacggtccg acaaggtcga atatgaacgt ctgttggcgt
     3301 ttttccgcaa cggcaacttg gaagaagccc ggcagggatt ttcgaccttc ttgacggagt
     3361 atcccaattc cgatcttgcc ccgaacgcgc gttattggct tggcgagtcg cattacggga
     3421 agaaggatta taagcaggcg atcgactcgt atgatcgtgt cgaactggat tacccgcaga
     3481 gcgagaaagt gccggctgct attttgaaga agggatatgc gtatctggcg cttaaagata
     3541 agaaacgggc ctcgtcggct ttcaagcagg tcgtgacgtt gtatcccaag agcccggagg
     3601 cagggaaagc gtatgacaaa ctcagtcagt tgaaggactc gcgatgatcg tgacgcaggc
     3661 gatcgtcagg gtcattggag caggtattgt ggtatggatg gctgtactgg ttgccggctg
     3721 tgccaaacat gccgatttca tcgagctgcg cgaccacctg gctacggtgt ccaagtcgca
     3781 agatcaagat caaaagcggc tggatgcgtt gcagcgacgc atggagtcgt tggaacgcat
     3841 caaagatgcc gaaccgacga agacgaagct ggacgaaatt ctggcgcgtg tgcagaagtt
     3901 ggaggcccgc gtcgccaagc ttgatgaggg aacaggagtg gcggctgtgc cgaagggtga
     3961 accacttccg attgaccctg cacggccagg gaaaccgcag aagccagggg gctctcctga
     4021 agggggctcg atcgttccca acgtccccgc aatcactccg acctctgcct tcaatctttc
     4081 gtataatgac tacctgaacg gcaaatatga tttggcggta gcggggtttc agcgattcgt
     4141 gaaagatttc ccaggaacct cgctgacccc gaacgcgtac tactggctgg gggaatcgta
     4201 ctaccagcag aaggactatg tccgcgctat gcagtcgttc gagtatgtcg caacggagtt
     4261 tccgggaaat gaaaaggtgc cggccgcatt gtataaactc ggacttgcgg cagcagagac
     4321 cggagacctg gtcaagtcga agaagaatct taaaagggtc cttgaagaat tccctacatc
     4381 ggatgaagcc aagctggcga agaataaatt ggccgaaatt cgatgatcga tcgtttcctg
     4441 gcacgcgacc agaagtaccg ctcgccttcc tgccttggag tctaacctga tgccagccgc
     4501 aagcgatgtg accaccattc gagtcctgcc gggtgaggtc gtcagccgca ttgcagccgg
     4561 tgaagtgatc gagcggccgg ccgcggtcgt caaagagctg atcgaaaaca gtctcgacgc
     4621 gggcagccga catatttcga tcgacgtcaa agacggcggt gtggcgctca tccgtgtgac
     4681 ggacgatgga gaagggatca gacgggcgga tctccccctg gccttcgagc gacatgcgac
     4741 aagcaagctg aagtcggacg aagatcttgc ctccatcaga acgatgggtt ttcgcggtga
     4801 ggcccttcca agcattgcct ctatctctaa ggtacaggtg acgaccgtaa ccaggcggga
     4861 aaagctgggt gcgcaattca ccatcctcgg cgggatgtcg caagggatcg cggatgcccc
     4921 gccgatgccc ggaacgaaaa tcgaagtatc ggatctgttc tacaatcagc cggcacgaaa
     4981 aaagtttctc aaatccaccg ttacggagtt ttcccatatc agcaatgtgg tccagcaggc
     5041 cgggttggga tggccttccg tacattttcg cctgacacac aatggccatg agatcatgaa
     5101 ttatcccgcc gtggcatccg accgagaccg gattcttcaa gtgtatcgat cggcctttct
     5161 ggataaggcg atcgacctcc gcggccgaat ggccggttgt gcgatcagcg gggtcatcat
     5221 cgatcccgtc catgcacgtt catcgcgggt tccccaagat ctgttcgtca atcggcggcc
     5281 gatcagaaac gccacggtgt ttcatgcggt gaccgacggg tttggatcgt ttctcccgaa
     5341 agggcatcac ccgacgttcg tgctgttcct cgacattgat cccgagcgaa tcgacgtgaa
     5401 tgttcatccg gccaagcgag aaatccgatt cgcggatcag gaagccattc atcaatttgt
     5461 gcgccatgcg gtgcggcatg cgctcagcgg accggagcga aaagtggtgc tcaccgtggg
     5521 aggaagcgaa gccttgccgc gcgctgattc cccttcggtg catccggcaa ggagggttca
     5581 agatcggcca gtgccttccc agtccgccca aggtgctcat gacgacccgg ccgtggtgac
     5641 cggttctcaa tcattgttca ctcatgaggc aatggcctct tatgccgatt cgtcggctcc
     5701 tgctgttgtt cctctgggcc aaatgcagag gacgtatctg gtggctcagg tggggcagga
     5761 attacaggtg atcgatcaac atacggcgca cgaacgcgtg ctgtttcaac ggttgtggaa
     5821 aaagtggctg aatcgtgaga tagccacaca acccctgctc attcctgagc cgattgaact
     5881 cgcggccgcg cagagtatgc ggttgcagaa acacctcgga gatttggaac agctgggctt
     5941 aatcatcgaa ccattcggat ccggtgcggt cgcgctccgg gccgttccgg cggggctggg
     6001 ccagatgaat ggcgggctat tagtgcagga tctgcttgag gatctgacgg agtgggacag
     6061 cgcctcgtcg ctggaagctc gtgtccagcc gcttttggca tctctggcct gccggagtgc
     6121 cgtgcaggcc gggcggagca tggcattgcc tgaaattgaa caactggttc gagattgggt
     6181 ggaagagggg ctcatcatga cctgcccgca cggaagacgg acggccttcc gtctctcgac
     6241 tgacgaactc gacaaactct ttggtcgggt cggatggtcg tgacgaccat accgattctc
     6301 accatgtatc cactgtcgga aacactgatc catctcaagc cgctggtcgt catcgtcgga
     6361 ccgactgccg tcgggaagag ccgcatcgcc atcgaggtcg ccaagcggtt tgagaccgag
     6421 attgtgaccg ctgactcccg tcaggtctat cgcgggatgg acattggcac cgacaaacca
     6481 cacgaggaga gccggcaagg gattccgcac caccttattg accttgtcaa tccggatcat
     6541 tcgttcaacg caggactgtt tcgacgtcat gcagtcgaga ctattgagcg gctctatcaa
     6601 cgtcggcagc tgcccctcgt tgtcggcgga accggcctct atgtgcgaac cttgctgcaa
     6661 ggactctgtg aggcaccacc ggccgatccg gcggtacggg cgcagctgca cgaggaagcg
     6721 cgcacgcaag ggcacgatcg cttctacgcg cgcctcgtgg cggtcgatcc ggtgaccgcg
     6781 tcgaagctgc acccccgtga tacgtccaag gtgattcgtg ctctggaagt gcaccaactg
     6841 tccggtcggg cgatgtcgga gtttcagcag cggcatggat tttccgaacg gccctattca
     6901 ccgctggtaa taggactgaa tcgagaccgt ggagcgctct atcgacgtat tgaggaacgg
     6961 atcgattggc agcttgctca cggactgttg caggaaaccc agcggttgct cgatcaaggc
     7021 tacaagcgca attgcaccgc gatgaagggt ctcggttatc ggcaggttgc agctcacttg
     7081 gctggtgagt atgaccaagc cgaaatggtt cggcgattca aacgagatac gaggcatttc
     7141 gccaaacggc agatgacctg gttcagacgg gaaccggcaa tcagctggct gatggtctcg
     7201 gaatcagaag ccaccgcgga gacggtcgat cgcgtcattc atcgggtcga acagttctta
     7261 gcgggcctcg atggggtgga tgcaggccag cgcagtgtcg aagagaacag gagatagcga
     7321 caatatgggg aaacgcattc cggcatctcg ggccgccatt ggaatcattg gcgggagtgg
     7381 gttgtatgac atcgaaggtt tgcgtcacgt aaaacacgtc cccgtgagaa caccgttcgg
     7441 gcagccatcg gatgacattg tgcttggaga attagaaggc gttcgcgtgg cgttcctgtc
     7501 gagacatggg cgcgggcatc gtctcaatcc atcggacatc aattatcgag cgaatgtcta
     7561 tgccatgaaa tccatcgggg tgcggcgcat catctccgtg agcgccgtcg gtagcatgaa
     7621 agagtcgatg aagccgggtg atgtggtatt gccggatcaa tttatcgatc ttacgaaacg
     7681 gcgcatctca accttcttcg aggggggtgc ggttgcccat gtcgccttcg gggagccggt
     7741 ctgcgcctcg ctgggcgcgt ccctgctggc tgccgcccgt tcgactggtg cgacggtgca
     7801 tcagggcgga acctatatct gcatcgaggg gccgcaattt tcgaccaagg cggagtcgaa
     7861 tctttaccgg caatggggcg tgagtgtgat cggcatgacg aatctgccgg aagcgaagct
     7921 tgcccgcgag gcggaactct gctacgccac agtggcgttg gtgaccgatt acgactgttg
     7981 gcacgagaca gaagaagcgg ttaccgtcga ggcgatcttg gcgacgctcc gtcagaacgt
     8041 ggcattggcc aagcggcttc tgcggggagc ggtggcttcc gccgcttcag tcgagagctg
     8101 tggatgtcat ggggcactgc aaaacgcgct cgtcacggat cccaatcgca ttccggccgc
     8161 tcttcgccgc aaattggcgc ccctcacgga gagggttctg tcgccgaaaa aaggaacaag
     8221 gtgaatcaat ggggaaactg cttgtcgtcg gatcggtcgc gttggatacg gtcaagacgc
     8281 cgttcggtga ggcagcggag gtgctgggag gatcagcgac ctatttttcc acctctgcga
     8341 gttattttac agccgttgat ttgattgccg tggtcgggga tgacttcccc tcccaacacc
     8401 tcaccttctt gaagagccgt gggattgatt tgaccggact ggagcgtcgg tccggtgcca
     8461 cgtttcgctg gaaaggcgag tatacgcatc aactcaatga agcgcatacg ctggatacca
     8521 aactgaacgt cttcgaaacg tttcgaccga agatccctga gtcctatcgg tcccccgaag
     8581 tccttttcct cgggaacatc gatccggaat tgcagttgga tgtgctgcac aagctccccc
     8641 ggcctccgtt agtggcctgc gacaccatga atttttggat taatgggaag cgcgatgcgt
     8701 tatggcgtgt actcgaacag gtcgatatct tgatcatcaa cgacggcgaa gcgcgtgcat
     8761 tagggggcga ctcgaattta gtgaaagtgg cgcagaaagt tctggcccgg gggcccaagc
     8821 acctgatcat caaacgtgga gagtatgggg tgctgatgtt caacgagaag caggtctttg
     8881 gcgccccagc ctttccgttg gaagacgtgc gtgatccgac cggcgccggc gatacctttg
     8941 ccggcggttt cctgggccat ttggccgcga cgggaaatcg gaatgtggag tccttcaagc
     9001 aagcgatcgt gttcgggagc gtcatggcct catttaccgt agaatccttt agtcttgacc
     9061 gtttgcgagt cctggattac aaagagattc aagaaaggtt ccgtgccttc aagcgtctga
     9121 cccattttga ggatcttccg tgacatttat cccagccagg tttcccctcc gccgctgtcc
     9181 gatcctggcc gttgtcgtcg cagtagggct gctgagcggg tgcgcgacct ctgaagaggc
     9241 gaagcagcgg tcaagggggt attaccagga ggggatggcg agtattgata ccgaccgtca
     9301 aaaggcattc gtctcatttc agaaggcagt acagatcgat cctgataaca aggaagcccg
     9361 gtacgggctt gggcatattc ttgtgtacca gggcaagttg tcccaggcgg aaagggagtt
     9421 tcgggaagcc atcaggatcg acgaagacta ctcagaagcc catacctacc tgggccaggt
     9481 tttggcgagt caggatcgtt ggcctgaggc gatagcggag tttcgcaagg cgctgaataa
     9541 cccgctctat cccaccccgg atctcgcgcg ttttcatctg ggcagggcgt tggcacatga
     9601 gggagatctt caaggtgcca tggaagcgtt ggaagatgcg caggctgtca acccgtcgag
     9661 cgtgccacca gcgctgttac agctggagtt aggacgggtc tactacaaga tgggctttga
     9721 gcgacgcgcc aaggatgcgc tgaccaaagt gacgaagatg gacaactccg gcgagtatgc
     9781 cacggctgca aaggaaattc tggcacggct gaagccgtaa agagagacgc atggaatcga
     9841 ttggagattt cttcaagcag gttcgggaaa ccaaggggct tacggttgat gaggtggcct
     9901 cgaagacccg tattcgatcg gatttcgtca aggcgttgga agaaggcaac tttgccaaac
     9961 tgccggatca agtattcgcc aggggcttcg tccgatccta tgcgcgctca ttggggcttg
    10021 atgaagagga tgccattcat cggttcattc aatcggctgg ggcattttat gaaaaacagg
    10081 atgagcgaga acgactgaag gcgagacagg cagaggaaga acgtagacgg caagcgaatc
    10141 gaaaggcggt cgcgatcgcg atcgggattg cgatcctgac gcttgtcgtg cttctaagcc
    10201 gagagcaatc ttccattctc gtgcggcgcg cgtccacgga gacgcctccg gcgtcgacca
    10261 aacgaaccgt gacgcctgag tccggttccc atgacgtgcc gtctcgtccg gaaggtgaag
    10321 gcgcaagctc taccctcaag ccaaaatcaa gcgagacccc ttcttcgtct acggcatcga
    10381 ggggcgatac gagtcccacg ccgcatggaa ctgccgcccc atctgttggt gcttctgccc
    10441 aacagcagac ggcgcttccc gttcctcctc cgatttccac cgacagcccg cttggaggga
    10501 cttcaccaga aagttcaccc aacgcagaag agaagttggt gttggatctt gaggcaacag
    10561 aactcagttg ggtcgttata cagatcgacg ggggcagtcc ccaagaggcc ctgcttcgcc
    10621 ccggagagaa agccaggtgg aaggggcagg atcaatttat cctgaccctt ggcaatgccg
    10681 ggggcgtgaa ggcggagctt aatggaaaac ctcagaaacc atttggtccc agtgggaaag
    10741 ttgcccggga tattctgctc aaacgctgat cttctttcct cgtatctctc aaaccacttc
    10801 ttctcgcatc ttgcgtgttt tgccattgac gtgtctgctc gctctcttgc tgctttctgt
    10861 ggcagggagg tcttccatgg ggcccaatgg tgtcaccagg aaatgagaaa acaagatcga
    10921 ccgaaagccc gcagagctaa ctaggcgaaa tatcgacggc tcggcgttga tcaatttggc
    10981 acagcgtttg attgagagaa gcaatgagga ggggaaaagg cagagaccgc ttgcggcgga
    11041 aggtgcctga gtcatcctca gggaggcaga caaggagggg tggtgccggc tccatttcca
    11101 tcagcaaacc ggggctggtt tgcttgacat taacggaagt gcgttgttat agtcccgacg
    11161 ttttttcgct ttagatagat atggggattg atgagtcagc ggccttgggc cgattctgta
    11221 tgtggtgggt acaacataaa ggaggatgtg tgatggggat tttctctaag ttcttgggaa
    11281 tcagcgcggc agtgctgatg ctgtccgcaa ctgtggtggg agccgaggaa aaggatcctc
    11341 tcaagccccg cgttccggcc gatcagattg ccgatgcaaa ggccatgaag aatccggtgg
    11401 ccgcaactcc ggagagcatt gccaaaggca aggcgctgtt cgaggggaag ggcacctgct
    11461 ttaactgcca tggcaaggaa ggcaagggcg atggtcctgc cggcgcgatc ctgaatccaa
    11521 gcccgcggaa cttcaccaac tgcaagtttc acaagaagcg gaaggacgga gagctgttct
    11581 gggtcatcaa gaatggcagc gcaggcacgg gaatggtttc gttgatcccc gctgcgatca
    11641 acgaagaaga agcctggacg atcatcaact acgagcggag cttctgcaag ggcgaagagt
    11701 agtctcgtag cgatttcagc atgaaggagg ggtggaggac ttgtcctcca cccttttttt
    11761 ttgccaaaac gagcagtttc ttcaggaggg aacaattctg ttgaggggaa cctcatcctg
    11821 gcctgttcga tcgcactggg acgtgcggac tcgggcagga gtatcgcctt ggtagagctg
    11881 gggttttacg cggctgagga gaaaagcgcg gttagggagt ttctcgtgcc tgtgccgtgg
    11941 ccagtctgct gagggggaag ctggctgcag gatcaccgtt ccggaattgt ggaaggacga
    12001 gcggacgttg ctcctgacca tactgtgtct tggatgccca gagcactttc tgcgtgaggt
    12061 ataagacgat ttctcccagt gtgacctcgc catcatgatt gctgtcagct tcgcctcgca
    12121 tggcgcgcag gaagtagtat gtgaagagcc cgtgtctgtg cttgtcgtct tcgatgatcc
    12181 gaccaatccc atttgaggca atgatatgga gagtcgagtt tcccgctggg ctccattgag
    12241 ggacagtgga tttggcccgg ctctctccgc ccagtctcgc gaccatgccg tcaaagagaa
    12301 gaatggtgtg cttggccttc aatcgtgcaa gggccgcttc cagatcttta agagggtagg
    12361 cgcgcgacgt gtttgtcgca tttccatcat aggggagtag gaacacctct cctgatgagg
    12421 acacggtggc aagtccggca aagtacacaa tgaccacggc ctctttgttc atatgaggag
    12481 gcaaccaatc gagcaaggcc tcatcaatat cggggcgcag agctttccag tcttgaagca
    12541 aacgcacatt cgacgcgggc agccctccga gcgattggaa gtagcttgtg aggatttcgg
    12601 cgtctgacgc cgcgtatttt ctaatcgaga ggtgctggtc gcggtaagag ccgaccccga
    12661 tcgataataa ataggtgtgg ggccgtttaa atccggcggc tgcagccggg acttggtcca
    12721 cgtcatcagc tgtgactcca gtcggttgga tcgacagcga gagggcttgc gaaggtggcg
    12781 cgccggctgc tgggtcaaat acggagacca ggagttcagc cttctgctgt tgcggacctt
    12841 gcgggagtgt cgcgacgaat tcgagtgagc gcgattggcc cggttggagc cgtccggccg
    12901 acagcgtggt ggctggaaat tgtgcaagca acgacggact gccggtaatg gttgcggtca
    12961 cgttttggag ttcttggctg cccgcgttca ccaggtcaac gcggacacga atgcgttccc
    13021 cactctcaaa cactaaattg ccgttctcgt ccagcatggt ggccttgaat gaaagcgtgg
    13081 aaggtgcacc tttcgcttcc acagccgctg gtggaggtgc tggccgcccg ggagatgatg
    13141 atgcagccac cttctccgaa ggggtactac ctgcagatgc caaaggggca ctccctgcgg
    13201 atgccaattg agagcgagcg gcaagaataa attgtacgga cagcttgaca cccgcgtcat
    13261 atacgaattc atccacattg ccgtagtcac aacgcttctg ggtcggttcc aaaatgagcc
    13321 gttcgttgtg tgcaatggtg atggtttgct ggccgagctc ttttcctgcc gcatccttga
    13381 aggttaagag cgcttcgaag gttatgtccg caggcacccg atcatagaga ttatcttgcc
    13441 agagtttgag ccccgagcgc tgcagcgtca caaggatctc cgtatcaggc gggataggtc
    13501 ctggggcgcc gccggtgacc gtcacggcct gaaagttctt ggcggctgca tctgtcagga
    13561 ccgattcgat ccctggtcca acgtcgagga tccgcaggct ggcgcagccg ttttggtact
    13621 gcatcttcag actcgtcaaa ctagggtcga acgtcaattt gacggaattg gggagacggg
    13681 ggcccatgtc gggcaggggg ctgcgcttga acacatcgca ccccccaagt ccacctgcag
    13741 cgaggaggag ggatagcaca accgcccact gcagcgtgag tcgttgaatt atttggtgcg
    13801 ggtattgtct gagaaccata ggccggggaa gttctatgtc gcgactggtt tggctgctaa
    13861 caatccacgg tctgcaccgt acctaaatgt ctgagcgaat gcaacaggga gaaggtgtct
    13921 tgcgacagtt ggaggcctca gctatcctgc gctttcgtag tcctgcgcgg cttaagtacg
    13981 ggcgatgatt cgatgctgac actcggttta tcgaacatgc gggatgccgc cgcggcgttg
    14041 gtagacaatg gccgggtgat cgctgctgcg gaagaagaac gatttgtacg ggtcaagcac
    14101 gtgacggctt tgccggttca cgccattcgg ttctgtctcc aggccgccgg gatccggttg
    14161 tgcgatatcg atgcggttgc agtgccatgg aaattttggc aattgggccg ccgtgcaagc
    14221 ctggctgtcg gctccatgct tcgctccccg cagctatttc gagtcaaagg gattcggagc
    14281 gtggaacggt tggctcacga atggaaagag ctggcgtttc ttcgctggta tctctcacgt
    14341 ctgatcgatg ggacagcctc cccgcctcct gtcttcctcg atcatcacct gtgtcatgcg
    14401 gccagtacag tgcttgtttc gccgtttgac cgtgcagcag tgctcatcgc cgatggagcg
    14461 tcggaagccc at
//
LOCUS       DATIFR010000069         1329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1961011, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000069 DATIFR010000000
VERSION     DATIFR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961011"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..838
                     /gene="fabG"
                     /locus_tag="VLD60_03875"
     CDS             98..838
                     /gene="fabG"
                     /locus_tag="VLD60_03875"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSB44123.1"
                     /translation="MSLTGKTALVTGAAQGIGRDIALALAADGADVAICDVNLEAAGK
                     TAADIEAKGRKSLALKANVAASAEVNAMIEEIVGKFGRIDILVNNAGITRDGLILRMK
                     DEDWDLVLSINLKGAFLCARAALKYMSKQRGGTVINIASIVGAMGNAGQANYVASKAG
                     LIGLTKTIAREYANRGITANAVAPGFIDTAMTQALPENVRQELAKQIPMGKLGTPEDV
                     ANAVRFLASPWASYITGQVIHVNGGMYM"
     gene            912..1148
                     /gene="acpP"
                     /locus_tag="VLD60_03880"
     CDS             912..1148
                     /gene="acpP"
                     /locus_tag="VLD60_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSB44124.1"
                     /translation="MADVDAKVKKIIAEQLGVPEGDVKPEASFVNDLGADSLDTVELV
                     MALEEEFGVEIPDEDAEKIATVGNAIEYIKAKAK"
BASE COUNT          336 a          331 c          414 g          248 t
ORIGIN      
        1 aacgtggagg accagaagag catgcgtgac acgctcggcg cgttgggact ataaaaaagt
       61 tcaaggttca gagttcaaag ttcgggagca atcctagatg agtcttaccg gcaaaacagc
      121 attggtcacc ggtgccgcac agggtatcgg cagggatatc gcgcttgcgc tcgcggcgga
      181 cggcgccgat gtcgccatct gcgatgtcaa tcttgaggcg gccgggaaga ctgcagcaga
      241 catcgaagcg aagggaagaa aatcactggc gctcaaggcc aacgttgctg catctgccga
      301 ggtcaacgcc atgatcgagg agatcgtggg aaaattcggc aggatcgata tcctcgtgaa
      361 caatgcaggc atcacccgcg acgggctcat tctgcgcatg aaagacgagg attgggacct
      421 cgtcctgagc atcaatctca aaggcgcttt tttgtgcgcg agggcggcgc ttaagtacat
      481 gtccaagcag cggggcggca cggtcatcaa catcgcctcc attgtcggcg cgatgggcaa
      541 cgccgggcag gcgaactatg tggcttccaa ggccggtctc atcggcctca cgaagacgat
      601 agcacgggag tacgcgaacc gcggcattac ggcgaatgcc gtggcgccgg gctttatcga
      661 cacggccatg acacaggccc tgccggagaa tgtcaggcag gaactcgcca agcagatccc
      721 catgggcaag ctcggcacgc ccgaggacgt tgcaaacgcg gtaaggtttc tcgcctcgcc
      781 ctgggcgtcg tacatcacgg gtcaggtgat ccacgtgaac ggcggcatgt atatgtaacg
      841 gcactgcgaa gaacagagtg cagagttcaa agttgaaagt ttaaaaagaa tattttaaga
      901 ggaggacgaa aatggctgat gtggatgcaa aggtaaagaa gatcattgct gagcagctcg
      961 gcgtgcccga aggggatgta aaacccgagg cctcattcgt gaacgatctc ggcgctgatt
     1021 cgctcgatac ggtggagctg gtcatggccc tggaggaaga gttcggtgtg gagatccccg
     1081 atgaggacgc tgaaaaaata gcaaccgtag ggaatgccat cgagtatatc aaggcgaaag
     1141 cgaaataagg attcaccggg cagcaccggt tcatgattca ggatttttca gaaagccaga
     1201 agacctctcc cgcttccgcg agagaggttt tttggctttt cggaccagag aggcaggagg
     1261 taaccgttct tgagaaaaag agtcgtcgtc accggcatcg ggatgatcac ccccgtcggt
     1321 cttgatacc
//
LOCUS       DATIFR010000070        15322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2726828, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000070 DATIFR010000000
VERSION     DATIFR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726828"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1506
                     /locus_tag="VLD60_03885"
     CDS             16..1506
                     /locus_tag="VLD60_03885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB44125.1"
                     /translation="MLVEAARKFSKPLLTSWMGHSQIIEGRGLFAQAGIPSFSTPEAA
                     VEAYSYITAYYRNQRLLAQVPPPLSHHDAPDVEGARMLIESVLAERRKVLSEMESKAL
                     LSAFHIPVAKTVVVRAPNEGLLMAEEFGFPVAMKINSPDISHKSDAGGVKLNLMNAAA
                     VRAAYHEIIEEVKAHRPDARVDGIAIQPMVRKPNGRELMVGVISDPVFGPVISFGAGG
                     TAVEVLGDRAIALPPLNNFLVKELISGTRIAKLLGAFRHMPPVQMEALESLLLRVSEM
                     VCELPWLKEMDINPLIVDEQGLLAVDARVVVDYAPVSADRYAHMAIHPYPAHLVTHWQ
                     LPDGTGVTIRPMRPEDAAIEQEFVRNLSEEAKYFRFMHAVQELSEDMLVRFTQLDYDR
                     ELALIAVTEEKGREVEIGVCRYAINPDGETCEFALVVSDQWQHKAIGHRLMGSLFDAA
                     RSKGLKTMEGEVLANNHNMLKLVATLGFTIATSPTDPSIKKITKVL"
     gene            complement(2031..2636)
                     /locus_tag="VLD60_03890"
     CDS             complement(2031..2636)
                     /locus_tag="VLD60_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HSB44126.1"
                     /translation="MRQPYIRSAMVLFMMIALTGCGSSPAATYHTLSPAAVMNGAAPA
                     SGESSYSVAVGPVTLPEPVDRPQLVLRVSPNEVTFVELHRWAGSLKTEIPRIIADNLA
                     AYLNVKRVAAYPQNAGDNADYRVLVDIQRFDSALGESVTIDALWTIKRQSDGALRTGR
                     STAKESSGGSYDAMVAAHSRALATISRDIAEAIRSANSSPQ"
     gene            complement(2633..4270)
                     /locus_tag="VLD60_03895"
     CDS             complement(2633..4270)
                     /locus_tag="VLD60_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSB44127.1"
                     /translation="MFDGTRGSVLEDVPSAVAVSKRRRSPQLVWLIPIIAALVGGGLV
                     VKTILEKGPTITITFKTAEGLEAGKTKVKFKNIDIGEVKTVGFTKDLQQVVVTAEMKK
                     GVKPHLVEDVKFWIVKPRIAGGQVSGIGTLFSGSYIEMDRGKSSTPREEFEGLELQPM
                     ITGDMQGRQFILQGKDLGSHDVGTPVMFRRIQVGKIAANELSQDGSSVTLKIFIESPY
                     DKFVTSNTRFWDVSGIDITADASGVSVNTEGLMSILLGGVAFEAPTGAGELPEADPDT
                     VFPLYPNRAAALKQPDRETLMFTMYFAESLRGLTVGSPLEVNSVTLGEVKGIKVEYND
                     ERGQFRFPVDVAVFPGRLRAMMRQGVAEPTPAEQKARLDAMIASGLRAQLKTGSLLTG
                     QMFISMDFFPKAAPAKIDWTKNPPVFPTEPGALVELQATLTNISKKIESMPLQQIGEN
                     LRIALASLNRTLMGMELAVKRMDKDVAPAAKAALEDARRMLNTADKTLSSDAPLQQDL
                     RTSLRDLSKAAQSLRELTEMLERQPESLIQGKKETKR"
     gene            complement(4263..4895)
                     /locus_tag="VLD60_03900"
     CDS             complement(4263..4895)
                     /locus_tag="VLD60_03900"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HSB44128.1"
                     /translation="MSDSEVTASQLGLCACHGCGGLSQLIPGIHEMRCRRCGAALHLR
                     KPNCISRTWALLIAAYIMYIPANLLPIMNTGSLFGSQSDTIMSGIVYLWHSGSWHLAL
                     IVFIASILVPLAKILALTYLLIAVQLGWREGALERTKLYRVLEFIGPWSMLDVYVVTI
                     LVALVQLKSLATIQPGPAAAAFGAVVVLTMFAAMAFDPRLLWEPKEAHHV"
     gene            complement(4892..5500)
                     /locus_tag="VLD60_03905"
     CDS             complement(4892..5500)
                     /locus_tag="VLD60_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HSB44129.1"
                     /translation="MNESALTACHECDLLQREPILPPGGVACCRRCNAVLFRHIPDSI
                     DRGLAYTLGAAILFIVANAFPIVGLEVQGTLNAASLYGAVEAIWDNNMEGVASLVFVT
                     TILIPAVEISLMLYVLLPLKFGRLPGEMASILRILQSVRPWSMTQVFILGVLVALVKL
                     AHLATVVPGVSLWAFGGLILLLTAAVNNFNTHELWSFLTVKE"
     gene            complement(5691..6539)
                     /locus_tag="VLD60_03910"
     CDS             complement(5691..6539)
                     /locus_tag="VLD60_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HSB44130.1"
                     /translation="MRPGQSIQTQSMQGAWILTCTKTIGWMQGSKGIVAALFSAAVIV
                     TGCARTVVQEGYESATIQLDRPSRILVYDFAVTREQVKENEGLLQGTINDFEGTTTYQ
                     HEGEIASEVRQVAAEEMVKGIQNLGLPAERAPAYASVPAGAVAITGQFLNVDEGNKAR
                     RLVIGFGSGKSRVDLRIQMFGYGLDRSNSSDVGPKKLVEFDTHADSGSMPGAAVTAGA
                     GAAAGAGAAAVVGVNAAVGGVKVYRSAMGSMTARSVEEAVDYLSEFFGRQGWIRPDKV
                     KSADRP"
     gene            complement(6645..8147)
                     /locus_tag="VLD60_03915"
     CDS             complement(6645..8147)
                     /locus_tag="VLD60_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSB44131.1"
                     /translation="MRHAILASLAFVAAVLPACMMGPDYTRPDTPKADSWRLTASTAE
                     SIANMPWWELLKDQALQTLIRASLQENLDLQIATANIEEFQAQLMIAKFDLVPSLNYS
                     ATAFGFRNTNENVFPAGGAGVQTGGKGGGLSVSYESADVGLKWEVDLWGRIRRSIEAA
                     RAQLLSKAENQRAVILGLVSTVAQTYFDLRGLDYEVEITKRTLKTWDESVRLSELRFK
                     QGVIPKLDLDRFQAERAGTAAQLADLERQVVQTENRLSILLGRKPSDIARGLHLTEQT
                     MPPAVPAGLPSDLLQRRPDILKVEQDLTATNATIGVAQATRFPQLALTGAVGGAGIRI
                     SGDSTGPFAIFKGSASLTGPIFNASALGYQVKSTEAQTQAAVAQYRKTVLTAFQEVED
                     ALIAVQKTGEQRTAQEQQVKALQSAYSLADFRYQGGRASYLDVLTAQRSLFDAELGLA
                     RTRRAQLTSVVQLYKALGGGWTPPGPGTTPQPVAATTDTAGPPAAMLVDR"
     gene            complement(8254..11523)
                     /locus_tag="VLD60_03920"
     CDS             complement(8254..11523)
                     /locus_tag="VLD60_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HSB44132.1"
                     /translation="MAKFFINRPIVAMVISIIMVILGVIAMVQLPIAQFPDIAPPEIQ
                     LQATYVGADAVSLEKSVATPIEQMMSGVDNMIYMYSVNASNGQMTLRVDFDINTRPND
                     DQILAQMRYTQSESQLPQDVRNFGVTIKKSTTSPLALFSLYSPNGTYDEIFLANYAYV
                     NINDPMTRVPGLGQVTIFGAGQYAMRFWVRPDQLAKLGITVTEILNAIKAQNTVNPAG
                     QVGMEPVPTGQEFTYTVRAQGRLETIEEFENIVIRANPDGSMVRLKDVARAELGAQMY
                     NIIGRMNGKPAAIIAIYQLPGSNAIQTMDAAKKLMEEMKSRFPADLDYVTSLDTTQAV
                     REGINEIVHTLFEALVLVIIVVYIFLQGWRATLIPLLAVPVSLVGTFVLFPMLGFSIN
                     TLSLFGLVLAIGLVVDDAIVVVEAVEHHIDHGLSPKEASLKAMEQVSGPVVAIALILA
                     AVFIPTAFIPGITGRLYQQFAVTIALSVVISAFNALTLSPALSALLLRPKKPARGPLG
                     VFFGWFNKWFGRATDGYVSFCGRLIRKAGLSMLFLLGVAVASGWLGGQLPTGFLPIED
                     QGFVYLNVQLPEASSLQRTDRLTREIEAILKDTPGVRYATSVIGYSLLSTVSTTYNAF
                     LFVTLDPWDERKKPEEQLLPIFKNVNARLAELPEAKAFVFPPPAIPGVGTSGGVTFIL
                     EDRSGRDIDFLAENTQKFMEAARKRPEISRIDTTFIPEVPQVFARVDRDKVLKQGVNL
                     SDVYQTLQAFMGGVMVNYFNRFGRTWQVYVEAEGEFRTKAENVGQFYVRNNSGDMVPL
                     SALVAMDTFHGPEFTMRYNEYRSSQLLVGSATGYSSGQAMTALEEVFKETMPAGMGFD
                     YMGMSFQEKVAAEGVPASVIFGFSLLFVFLILAAQYESWALPFSVLLVTPIAVFGAFG
                     ALWLLKLVAPQASENDVFTQIGLVMLIGLSAKNAILIVEFAKVELEGGKSIMDAALAA
                     AQVRLRPILMTAFAFILGVVPLVLATGAGAHGRVLLGLAVFGGMLAATVIAIFLIPVT
                     FAFVENLVHRGEPHAKPTPGAPLPGGPKGSGDGHPTQPIPQPTLSPTHARESTGYGGG
                     H"
     gene            complement(11582..12856)
                     /locus_tag="VLD60_03925"
     CDS             complement(11582..12856)
                     /locus_tag="VLD60_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB44133.1"
                     /translation="MALLLLDPLGQRLLQDVEMEEGTIAGFMNTLSRMKQGGRDLSKN
                     AGSALLIGLLALSLTHCKGETASAPPPPLLKVDVADVIQKDVPIQSEWVGTTDGMVNA
                     VIRAQVTGYLMKRPYTEGSFVKKGDLLFELDPSKFRAILNQAQGDLAKAEAQLLKTKQ
                     DVERDTPLAKAGAVSQKELDDSIQANAAAKGNTASAKAAVEQAKLNLGWTRITAPIDG
                     VVGIAKAQIGDLIDANSELTSMSTVDPIKVYFPLSEQEYLGAAEKVRERYKEMEKGQE
                     YGGAQVELILSGERVYPHKGQFYLVDRQVDVKTGTIRVAALFPNPDNLLRPGQFARVR
                     AVTKMREKALLVPQRAVTEMQGSYQVAVVTPENKVDIRPVKVGERAGNLWIIDKGLNP
                     GERVVVEGLQKLKAGMTVEPKPFQETPDEKTT"
     gene            complement(13210..14538)
                     /locus_tag="VLD60_03930"
     CDS             complement(13210..14538)
                     /locus_tag="VLD60_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSB44134.1"
                     /translation="MSSRLYQWLDSRLNLKSVERTLLDEPIPGGASWIYVFGSATLFL
                     FILQAITGMFLAVYYAPTPDHAYDSVQFIEHQVTFGWFVRGLHHWGASGMVVAVGLHM
                     LQVFLYGAFKPPREMMWMVGVVLFLLVMGFAFTGYLLPWDQTAYWATQIGINMVGTVP
                     LVGEFLMKVMRGGETLGALTLSRFFAVHVLFLPALIIMGIMLHLFILRRVGPAGPWDE
                     QKAKSGSETFYPRQVYMDAVVMLGVFGVLALLAVLVPFPLADKANPSDTSFVPVPEWY
                     FLFYYELLKHVHGPLEPFATWVLPAMAVLVMLLWPFIDRQKTVRAPASRRIGMTLAVL
                     FLVTVFSLLGISLKNLYAVKRIDPGVAHGQALVEHLGCTGCHRIHGTGGAVGPDLSYV
                     GDQRPDREWHLKHFRDPQSVSPGSIMPKFPLTDQELNDLTSYMLSLRRAT"
     gene            complement(14599..14985)
                     /locus_tag="VLD60_03935"
     CDS             complement(14599..14985)
                     /locus_tag="VLD60_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSB44135.1"
                     /translation="MEKPHKRLNVWKQAVELSLMINQATEKFPLEERYSITDQMRRAA
                     TSIATNIAEGAARNSKRDFARFLHMAQGSLSELDTQLEIVKRLGYLHDESWRILDDRM
                     NEIDKMLSGLIRHQISLHGKGGAKSK"
     gene            complement(15067..>15322)
                     /locus_tag="VLD60_03940"
     CDS             complement(15067..>15322)
                     /locus_tag="VLD60_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB44136.1"
                     /translation="VKQSDGQVTVFSPMCTHLGCGYHWDVTDRKFKCPCHGSVYDVTG
                     QVLAGPAPRHLDALPSKVDQGRLLVMYKEFKSGVSDQVEI"
BASE COUNT         3324 a         4609 c         4205 g         3184 t
ORIGIN      
        1 ctggaagtcg ccacattgct ggtcgaggcg gcaagaaaat tcagcaagcc gctgctcacc
       61 agttggatgg gtcattctca gattatagag ggacgaggcc tgttcgctca ggccggcatt
      121 ccctcattca gcacccctga agcggccgtc gaggcctatt cctacatcac cgcgtattac
      181 cgcaatcaac gcctgttggc gcaagtcccg ccaccgttat cgcaccatga tgctccggat
      241 gtagagggcg cgcgcatgct gattgagtct gttctggcgg aacggcgcaa ggtgttgtct
      301 gagatggaat ccaaggcgct cctgtcggcc ttccatattc ccgtggccaa aacggtggta
      361 gtccgtgctc ccaacgaggg gctgctgatg gcagaggagt tcgggttccc ggtggcgatg
      421 aaaatcaatt cgccggacat ctcccacaaa tccgatgccg gcggcgtcaa gctgaactta
      481 atgaatgccg ccgccgttcg cgcggcctat cacgagatca tcgaggaggt gaaagcacac
      541 cgtcccgatg cacgagtcga tggcatcgcg atccagccga tggtgcgtaa gcccaatggg
      601 cgcgaattga tggttggggt catctcggat ccggtgttcg gaccggtgat cagcttcggc
      661 gcaggcggca ccgcagtaga agtgctgggg gaccgcgcca ttgcgttgcc gcctctgaac
      721 aatttcctgg tcaaagaact cataagcggc acgcgcatcg ccaaactgct cggtgcgttt
      781 cggcatatgc caccggtgca gatggaggcg ctcgaaagcc tgcttctccg ggtgtccgag
      841 atggtgtgcg agctgccatg gttaaaggaa atggatatca atccgctgat cgtggatgag
      901 cagggtctgc tggcggtcga tgcgcgcgtc gtcgtggatt atgcgccggt ctcagccgac
      961 cggtacgccc acatggcgat ccatccgtac ccggcccacc tggtcactca ttggcaatta
     1021 ccggatggca ccggcgtcac cattcgtccg atgcgcccgg aagacgcggc gatcgaacag
     1081 gaatttgtgc gcaacctgtc ggaagaagcg aaatacttcc ggtttatgca cgccgtgcag
     1141 gagctgtccg aagatatgct ggtgcgcttt acccaactcg actacgaccg ggaattggcg
     1201 ctgatcgccg tgaccgaaga gaagggccgc gaagtggaaa tcggcgtctg tcgctatgcc
     1261 atcaatcctg atggagaaac atgcgaattc gcgctggtgg tcagcgacca gtggcagcac
     1321 aaggcgatcg gccacaggct gatgggcagc ttgttcgacg cagcccgcag caaggggctc
     1381 aagacgatgg aaggcgaagt cctggcaaac aaccacaaca tgctgaaatt ggtagccacg
     1441 cttggcttta ccatagccac cagtccaacg gacccctcca tcaagaagat caccaaggtg
     1501 ttgtagcaag caactgaagc atcttttttc agccgaccgg ctaacacctg cacggcacat
     1561 cgctcgtgcc aacctcactg tcactctcgc tgcttcaacc tctccggcac gtcggctacg
     1621 gtttctgcct cccgtttcct ccgtaaactg tgcgttgact ctcctctttc cgtgtgagag
     1681 ccaactgcat ttcaacaatg gatcaaatga tcctcaaacc ttgtcagctc gtgctgactc
     1741 gttactgaga aatccgtgag ggattggatg atcggttttt tagtggcgcg agcaaatgca
     1801 gcgaccggaa cggagaatta ctagattacc agcggagcga ggcgccacca gtggcatctg
     1861 gctttgtaac tggttgattg cccatgccac gaaatgtcac ttctatcatt attttctgct
     1921 tctaaatgat tgatctaact taatgttgtt tatgatatag aaaggctggc gtcgacagtt
     1981 cggttgtgtc cctcggcacc acaacccttc acaaccgaag aacgcgaagg ctactgtggg
     2041 gaagagttcg ctgaccggat ggcttcggcg atgtcccggc tgatcgtggc caaggcacgg
     2101 ctgtgtgcag cgaccatagc atcgtaactt ccgccgctgg attccttggc agtcgatcgt
     2161 ccggttcgca aagctccatc agactggcgc ttgatcgtcc agagcgcgtc gatcgtgacc
     2221 gactcgccaa gtgcggagtc gaagcgttgg atgtcgacga ggacacgata atcggcattg
     2281 tcccctgcat tctgcggata ggctgccacc cgctttacat tcaaataggc agccagattg
     2341 tccgcaataa tacgtggaat ttcggtcttc agcgacccgg cccatcgatg caactccacg
     2401 aaggtgacct cattcggact gacgcggagc acgagttgcg gacggtccac cggctcggga
     2461 agggtcacgg gacccaccgc cacactgtac gacgattcgc cgctcgcggg cgccgctccg
     2521 ttcatcacag cggcggggct gagggtatgg taggttgcgg cgggtgaact gccgcagccg
     2581 gtcagagcaa tcatcatgaa caacaccatc gccgatcgga tatatggctg cctcaccgtt
     2641 tagtctcttt cttcccttga atcagggact caggttgacg ctcgagcatc tccgtcagct
     2701 cgcggagcga ttgtgcggct ttcgagaggt cacgcagcga ggttcgaaga tcttgctgca
     2761 ggggtgcatc ggaagacagc gtcttgtcgg ccgtgttcag catgcgccgc gcgtcttcca
     2821 aggcggcctt cgccgccggt gcgacgtcct tatccatccg tttcacggct agctccattc
     2881 ccatcaatgt acggttcagc gaggccagcg ctatgcgaag attctcgccg atctgctgga
     2941 gcggcatact ctcgattttc ttcgaaatgt tcgtcaaggt agcttgcaat tccaccaatg
     3001 cgcccggttc cgtcggaaac accggcggat ttttcgtcca atcgattttt gccggagcgg
     3061 ccttggggaa aaaatccatg ctgataaaca tctgaccggt caggagacta cccgtcttca
     3121 gctgtgcgcg caggccgctc gcgatcatcg catcgagtcg agccttttgt tcggcaggtg
     3181 tcggttctgc caccccttgc cgcatcatcg ctcgcaggcg tccagggaaa acggcgacat
     3241 caaccggaaa acggaattgc cctcgttcat cgttgtactc caccttgatg cccttgactt
     3301 caccaagcgt cacactgttg acctcgagcg gagaacccac cgttaacccg cgcagggact
     3361 ccgcaaaata catggtaaac atcagcgtct cgcgatccgg ctgcttcagg gccgccgcgc
     3421 ggttgggata gagcggaaag accgtatcag gatccgcttc aggcagctcc cctgcgcctg
     3481 ttggcgcctc gaaggcgaca ccgccgagca ggatggacat cagcccctcg gtattgacgc
     3541 tgacgcccga cgcgtcggca gtgatgtcga ttccgctcac gtcccaaaag cgcgtgttgg
     3601 aggtcacgaa cttgtcatac ggcgactcga tgaagatttt cagtgtgacg ctcgagccgt
     3661 cctggctcag ctcattggcc gctatcttcc ccacctgaat gcgccgaaac ataaccggcg
     3721 ttccgacgtc gtgcgacccg aggtctttac cctggaggat gaactggcgg ccctgcatgt
     3781 cgccggtgat catcggctgc aattccaggc cttcaaattc ctcacgcgga gtcgaggatt
     3841 tgccgcgatc catctcgatg taggatccgg agaagagcgt cccgatcccc gagacctgcc
     3901 cgcccgcgat ccgcggcttg acgatccaga acttgacatc ctccacgaga tgaggcttga
     3961 ctcccttttt catctccgcc gtcacgacga cctgctggag atccttggtg aagccgacgg
     4021 ttttcacctc gccgatgtcg atgttcttaa acttgacttt ggtcttgccg gcctcgagtc
     4081 cttccgccgt cttgaaggtg atcgtgatcg tggggccttt ctcgaggatt gttttcacca
     4141 ccagcccgcc tccgacgagg gcggcaatga tcggaatcaa ccacaccagt tggggagacc
     4201 ggcgtcgctt agaaacagcc accgccgatg gaacatcttc caaaaccgac ccgcgcgttc
     4261 cgtcaaacat gatgagcctc ttttggttcc cagagcagcc gaggatcgaa cgccatcgcc
     4321 gcaaacatcg tcaagacgac cacggcgccg aacgccgccg cagccgggcc cggctgaatc
     4381 gtcgcgagcg acttgagttg gacgagcgcc acgagaatcg tcaccacata cacatccagc
     4441 atcgaccagg gaccgatgaa ctcaagaacc cggtagagct tcgtccgctc cagggccccc
     4501 tccctccatc ccaactgcac tgcaatgaga agataggtga gggcaagaat cttcgcaagc
     4561 ggcacgagga tgctcgcaat gaagacgatc agcgccagat gccacgaacc cgaatgccaa
     4621 agatagacga tgccgctcat gatcgtgtcg gattgagagc cgaacagcga accggtgttc
     4681 ataatcggca agagattcgc cggaatatac atgatatacg cggcgatgag taacgcccac
     4741 gtccgcgaga tgcagttggg tttgcgcagg tggagtgccg cgccacagcg gcggcaacgc
     4801 atctcgtgga ttcccggaat cagctgggac aagccgccgc atccgtggca ggcgcacaat
     4861 cccaactggc tggctgtcac ctcagaatcg ctcattcttt caccgtcagg aaactccata
     4921 actcatgcgt gttgaagttg ttcaccgcgg cggtcaacaa caaaatcagg ccgccgaatg
     4981 cccagagcga gacacccgga accaccgtgg ccaggtgggc aagcttgacc agcgccacca
     5041 gcacgccgag gataaacacc tgcgtcatac tccacggcct gacggattgc aggatacgca
     5101 ggatcgacgc catttctccc ggcagcctcc cgaacttcaa cggcaggagc acatacagca
     5161 tgagagaaat ttctacggca gggatcagaa tagtcgtgac aaacaccagg gaagccacgc
     5221 cttccatatt attgtcccag atggcctcga ctgccccata caggctcgcc gcattcaacg
     5281 taccctggac ctccaagccg acgatcggaa acgcattggc aacgatgaag aggatcgctg
     5341 caccgagcgt ataggccagg ccgcgatcaa tactgtcggg aatatgacgg aagagaacgg
     5401 cattgcagcg ccggcaacag gcaacgccac ctggcgggag gatcggttcc cgttgcaaga
     5461 gatcgcattc gtgacaggcg gtcaacgccg actcattcat gcaagattct ccaccgttca
     5521 agtctccgtg cccaagggac aacgaacacg ctccgggacg atcacgagag ccgggaacat
     5581 ccgaccagcg ggcattttcc tgatcccgtt gccctcgcca gtagattcta gagacatctg
     5641 gtcatacctg caacaggagg atattgttcc gaggcctttg cccgtctggc tcagggacga
     5701 tctgcggact tgaccttatc cggtctgatc cagccctgcc ggccaaagaa ctcggacaag
     5761 tagtccaccg cctcctcaac actcctggcc gtcatcgatc ccatggctga ccggtacact
     5821 ttcacgccac ccactgccgc atttaccccg accactgccg cagccccggc acctgctgca
     5881 gcgccagctc cagccgtcac tgctgctccc ggcatagacc cgctgtcggc atgggtgtcg
     5941 aactccacca gcttcttcgg cccaacatcg gatgaattcg aacggtccaa gccgtatccg
     6001 aacatctgaa ttcggaggtc gaccctcgat ttccccgacc cgaatccgat caccagccgc
     6061 cgggctttgt tgccttcatc cacattcaga aactgtcctg tgatcgccac cgcccccgcc
     6121 gggacagatg cataggcagg ggcccgctcc gccggaagcc cgaggttctg aatccccttg
     6181 accatctctt cggccgcgac ctgccgaacc tcgctggcga tttctccttc gtgctgatag
     6241 gtcgtcgtcc cttcgaaatc attgatcgtc ccctgaagca gaccctcatt ttccttcacc
     6301 tgctctcgcg tcacggcgaa gtcatacaca agaatgcgcg acgggcgatc gagttgaatc
     6361 gttgcacttt cgtacccctc ctgcaccacc gtccttgcgc agccagtgac gatgacggcc
     6421 gcgctgaaca gggctgcgac gattcccttg ctcccctgca tccatccaat tgttttggta
     6481 caggtcaaaa tccaggcccc ctgcatcgac tgagtttgaa ttgattgacc tggccgcata
     6541 cagagtctcc tttgccaaag ggaactcatc gtactcattt cataaaacct tgtgatctcc
     6601 cattcgctcc gagaacggtc attgcctcag cgagaatagc gaacctaccg gtcaacgagc
     6661 atcgccgcgg gcggcccggc ggtatcggtt gttgcggcga caggctgcgg tgtggtcccc
     6721 ggtcctgggg gagtccatcc tccgccaagt gctttataga gttggaccac ggatgtcagc
     6781 tgcgcgcgcc gcgtgcgggc caacccgagt tccgcatcaa acagactgcg ttgggccgtc
     6841 agcacatcga ggtaactggc ccgtcctcct tgatagcgaa aatcggcaag gctataggcg
     6901 gattgcaggg ccttcacttg ctgttcctgt gccgttcgct gctctcccgt cttctgcacg
     6961 gcaatcaatg catcctccac ttcctggaat gccgtcagaa ccgtcttcct gtattgtgcc
     7021 actgcggcct gagtctgcgc ctccgtggac ttgacctgat agcccaatgc cgacgcattg
     7081 aaaatcgggc cggtcaacga ggcagaacct ttgaagatgg cgaacggccc agtggaatct
     7141 cctgagatgc ggattcctgc tcccccgact gcacccgtca gagcaagttg cggaaacctg
     7201 gtcgcttgcg ccacaccgat ggtcgcgttc gtcgccgtga gatcctgctc gactttcagg
     7261 atgtcaggac gccgttgaag gagatcggac ggcaatccgg ccggaacggc aggcggcatc
     7321 gtttgctcag tcaggtgaag ccctctcgcg atatccgacg gctttcggcc gagcagaatg
     7381 ctgagccggt tctccgtctg gacgacctga cgctccaggt ccgccagttg agccgcggtt
     7441 cccgcccgtt ccgcctgaaa tcgatcgaga tcgagcttcg ggattactcc ctgcttgaaa
     7501 cgaagctctg acaaccgaac ggattcatcc catgtcttga gggtgcgctt ggtgatttcc
     7561 acctcgtaat ccagtccccg gagatcaaaa tacgtttggg ccacagtgct gaccaatccg
     7621 agaatcaccg ctcgctgatt ttccgctttg gacagcaatt gcgcccgggc cgcctcaatc
     7681 gatcgccgaa tgcgccccca gagatcaact tcccacttga gaccaacatc ggcgctttcg
     7741 tacgagacgc tcaatccccc gcctttcccg cctgtctgaa caccggcgcc gccggccgga
     7801 aagacgttct cattggtgtt ccgaaagccg aaggccgtcg ctgagtaatt caacgaggga
     7861 accagatcga acttggcgat catgagctgc gcctgaaact cttcgatatt ggctgtagca
     7921 atctgcaaat ccaggttttc ctgcaacgaa gcgcggatca atgtctgcag cgcctgatcc
     7981 ttcaacagtt cccaccaggg catgttcgcg atggactcag ccgttgatgc tgtcagacgc
     8041 caggagtccg ctttcggggt atcagggcgg gtataatctg gccccatcat gcaggcgggc
     8101 agcaccgccg cgacaaatgc gagactagcg agaatcgcgt gacgcacaag agagcttcgt
     8161 ggcccccatg tttcttgcct gtcgcgcctt tcgcgcgagt ctcgcctgtc ccgcctgtcg
     8221 cgcaactctc gcttttctcg cgtcaggtcc atctcagtgc cctcctccgt aacctgtgct
     8281 ctcacgagca tgggtcggcg acagtgtcgg ctgcgggatc ggctgcgtcg gatgcccgtc
     8341 gcccgatccc ttcggccccc ctggaagagg tgcgcctggc gttggtttcg cgtgaggttc
     8401 gccgcgatgc acgaggttct ccacaaaagc gaacgtcacc gggataagga aaatcgcgat
     8461 gacggtcgcg gccagcatgc cgccgaacac ggcgagacca agaaggactc gtccatgcgc
     8521 tccggctccc gtggcgagca cgaggggcac gacgccgagg atgaatgcga aagcggtcat
     8581 gaggataggc cggagccgca cctgggccgc agccagggcg gcgtccatga tggacttccc
     8641 tccttcaagc tcgactttcg caaattccac gatgaggatc gcgttcttgg cagatagccc
     8701 gatcaacatc accaggccaa tttgagtaaa cacatcgttc tcactggcct gaggagccac
     8761 aagcttcaga agccacaggg cgccgaacgc gccgaagacg gcgatcggcg tgacgagaag
     8821 cacgctgaag ggcaaggccc aactctcata ctgcgccgcg agaatcaaga acacgaatag
     8881 cagagagaac ccgaaaatca cgctggccgg cacgccttcc gctgccacct tctcctgaaa
     8941 cgacatgccc atatagtcga atcccatacc ggccggcatc gtttccttga agacctcttc
     9001 cagcgcggtc atggcctggc cggaactata gccggttgcc gatcccacaa gcagctgaga
     9061 tgaacggtat tcgttgtagc gcatcgtgaa ctcggggcca tggaaggtgt ccatcgccac
     9121 cagcgccgag agcggaacca tatccccgga gttgttgcgg acgtagaatt gtccgacgtt
     9181 ttcggctttc gtgcgaaact ctccttccgc ttccacatac acctgccagg tgcgaccgaa
     9241 acggttgaag taattcacca tcactccgcc cataaaagcc tgcaatgttt ggtacacgtc
     9301 ggagaggttc actccctgct tgagcacctt gtcccggtct accctggcga acacctgagg
     9361 aacctcaggg atgaacgtcg tatcgattct cgagatctca ggacgcttcc tggcagcctc
     9421 catgaacttc tgggtattct ccgccagaaa atcgatatcc ctcccggagc ggtcttccag
     9481 gataaacgtc acaccaccgg acgtaccaac acccggaatc gcgggcggcg gaaacacgaa
     9541 ggccttggcc tcaggcaact cagccagtcg tgcgttgacg ttcttgaaaa tcggcaggag
     9601 ctgctcctca ggcttttttc gctcgtccca ggggtcgagc gtgacgaata agaacgcatt
     9661 gtaagtcgtg ctcaccgtgc tcaagaggct gtagccgatg acgctggtgg catacctgac
     9721 gcccggcgtg tcttttaaaa tggcctcgat ctctctggtc aaccggtcag ttcgctgcag
     9781 agaggacgcc tcagggagct ggacattcag gtacacaaag ccttggtcct cgatcggaag
     9841 gaaaccggtc ggcaattgac cccccagcca gccggacgcg acagcgactc cgagaagaaa
     9901 gagcatcgag aggccggcct ttcgtatgag acggccgcag aaactcacgt agccatcggt
     9961 cgcgcgcccg aaccatttat taaaccagcc gaagaaaact cccagcgggc ctctcgccgg
    10021 tttcttcggt cggagtaaga gagccgagag ggcggggctg agcgtgaggg cgttgaaggc
    10081 cgagatgacc acggacaggg cgatggtgac ggcaaattgt tgatacagtc ggccggtgat
    10141 ccctggaata aacgccgtcg gaatgaagac cgctgccagg atcagggcaa tcgccaccac
    10201 aggcccggac acttgttcca tagccttgag actcgcctcc ttcggcgaca ggccatgatc
    10261 gatgtgatgc tcgactgcct cgaccacgac gatggcatcg tccacgacca agccgatggc
    10321 cagcacgaga ccgaagagcg acagcgtatt gatcgagaaa ccaagcatcg ggaacagcac
    10381 gaatgttcca accagcgaga ccggtacggc cagcaacggg atcaacgtgg cccgccagcc
    10441 ctggaggaag atgtacacga cgatgatcac cagcaccagg gcttcgaaga gcgtgtgcac
    10501 aatttcatta ataccctctc gcacggcctg cgtcgtatcg agcgaggtca cataatcgag
    10561 gtccgccgga aaccgcgact tcatctcctc catcagtttt ttcgccgcgt ccatggtctg
    10621 gatcgcgttg gatcccggca actgatagat ggcaatgatc gccgccggtt ttccgttcat
    10681 ccggccgatg atgttgtaca tctgcgcacc gagctcggct ctggccacgt ctttcaatcg
    10741 caccatggag ccgtccgggt tggcccggat cacgatattc tcgaattctt ctatcgtctc
    10801 caaacgaccc tgcgcgcgaa cggtgtaggt aaactcctgt cccgtcggca ccggctccat
    10861 gccgacctgg cctgcagggt ttacggtgtt ctgggccttg atggcgttga gaatctccgt
    10921 cacggtgatg cccagcttgg cgagctggtc cggccgcacc cagaaacgca tcgcatactg
    10981 gcccgccccg aagatcgtca cctggccaag gcccggcaca cgggtcatgg gatcattgat
    11041 gttgacgtag gcatagttgg ccaggaagat ttcgtcgtag gtcccgttcg gggaatataa
    11101 cgagaacagc gcaaggggac tggtcgtgga tttcttgatc gtgaccccga agttccgcac
    11161 gtcctggggc aattgggatt cggactgggt atagcgcatc tgtgccagga tctgatcgtc
    11221 gttgggcctg gtgttgatat cgaagtccac acgaagcgtc atctgcccgt tgcttgcgtt
    11281 gaccgagtac atgtagatca tgttgtccac cccgctcatc atttgctcga tgggagtggc
    11341 aaccgacttt tcgagggaaa ccgcatcggc cccgacatag gtggcttgca gctggatttc
    11401 aggtggcgcg atgtccggaa actgcgcgat cgggagctgc accatcgcga tgacgccgag
    11461 gatcaccatg atgatcgaga tcaccatcgc gacgatcgga cgattgatga agaacttggc
    11521 catagaaatg aggagacaga ttcagcggca gtagcggaga aacgtcagat aggcatctac
    11581 atcacgtcgt cttttcatct ggcgtctcct ggaacggttt aggttccacg gtcatgccgg
    11641 ccttgagttt ttgcaaccct tcgacaacca cgcgctcgcc cggatttaac cctttgtcga
    11701 taatccagag atttcccgct cgctccccga ctttgacagg gcggatatcc actttgttct
    11761 ccggagtcac gactgccacc tgataacttc cctgcatttc ggtgaccgca cgctgaggta
    11821 cgagcaacgc cttttccctc atcttcgtca ccgcccgcac gcgagcaaat tgaccgggcc
    11881 tgagaaggtt gtcggggttg gggaagagcg ccgccacgcg aatggtgccg gtcttgacat
    11941 ccacctggcg atcgaccaga tagaactgtc ctttatgcgg ataaactcgt tcgccgctca
    12001 ggatcaattc gacttgcgct ccaccgtact cttgcccctt ctccatttcc ttgtagcgtt
    12061 cccgaacttt ttccgcggct ccgagatatt cctgctcgct gagcgggaaa tagaccttga
    12121 tcgggtctac cgtcgacatc gacgtcaatt cagagttggc atcaatgaga tcgccgatct
    12181 gggccttggc aatgccgacc actccgtcga tcggcgccgt gatcctggtc caacccaaat
    12241 tgagtttcgc ctgctccacc gccgctttgg cagaagcggt gttacctttt gcggctgcat
    12301 ttgcttgaat ggaatcgtcc aattctttct ggctaacggc tcctgctttg gcaagcggcg
    12361 tatcacgctc cacatcctgc ttggttttca agagctgcgc ctctgctttg gccaaatcgc
    12421 cctgagcttg attaagaatc gcccggaatt tgctgggatc caactcgaac aacagatcac
    12481 ccttcttgac gaacgatcct tcggtataag gccgcttcat gagatacccg gtgacctggg
    12541 ctcgaatgac ggcattgacc atgccgtccg ttgtgcctac ccattctgac tggatgggca
    12601 catctttctg gatgacgtcg gcaacgtcga ccttgagcaa gggaggagga ggtgcagagg
    12661 cagtttcacc tttgcagtgg gtgagactca gcgccaacag gcctatcagt aaggccgacc
    12721 ctgcgttctt agacaaatca cgtccccctt gcttcattcg gctgagcgta ttcatgaagc
    12781 ccgctatagt cccctcttcc atttcaacgt cttgcaacag tctttgacct aagggatcga
    12841 gcaagagcag tgccattagt gcctgattgc aaggtggtca tgagtttcct tcggatcctt
    12901 gcattctcta gaagttctga ggaagcacac cctgatgaat gagaggcaac tggggaggat
    12961 ggcaacagaa ggaacgtagg acatagtccc aaatctcctc aatcaaatcg cctctaccgt
    13021 ccgccgctcg ggcccgtcca ttgccgcaac gcggccatct tcgccactcc gagggtaggg
    13081 actttacctg agatctgcaa cagatagcaa cggctgggta aggggcagtg aaggaggacg
    13141 aatatgcgga cactcagcct aataggaaat aaccgtgcca gcctctgaca agctatgaga
    13201 tacggggaat caggtcgctc gacggagact cagcatgtag ctcgtcaggt cattgagttc
    13261 ctgatcagtg agggggaact tcggcatgat ggaaccgggc gatacagact gcggatcacg
    13321 aaaatgcttg agatgccatt cccgatcagg gcgttggtcg ccgacatagg aaagatccgg
    13381 tccgacggct ccaccagttc catgaatccg gtgacatcct gtgcaaccga ggtgctccac
    13441 caacgcttga ccgtgcgcga cgccagggtc aattcgcttc accgcgtaaa gattcttcaa
    13501 tgagatgccg agcagagaga agacagtgac cagaaataat acggccaagg tcatgccaat
    13561 ccgtcgtgag gccggagcgc gcacggtttt ctgtcgatcg atgaacggcc agagcaacat
    13621 cacgagaaca gccatcgctg gtaaaaccca tgtggcaaag ggttccagtg gcccgtgcac
    13681 atgcttgagc aactcatagt agaagaggaa ataccactcc ggcaccggca caaagctggt
    13741 gtccgacgga ttggctttat cggcaagcgg aaagggaacc agcacagcca gcaaggccag
    13801 caccccgaaa acacccaaca tgaccaccgc atccatgtag acctggcgtg gatagaaagt
    13861 ctcgctgccg gacttggcct tttgctcatc ccagggtccg gccggtccca cccgccggag
    13921 aatgaagagg tggagcatga tacccatgat gatgagggca ggcagaaaga gcacgtgaac
    13981 cgcaaagaat ctggagagag tcagcgcccc cagcgtctct cccccgcgca taaccttcat
    14041 gagaaactcg ccgacaagag gaaccgtccc gaccatattg atgccaatct gtgtggccca
    14101 gtatgccgtc tgatcccagg gcagaagata tccggtgaag gcgaatccca tcacaagcag
    14161 aaacaacacc acgccgacca tccacatcat ctcgcgcgga ggcttgaatg ccccgtagag
    14221 aaacacctgg agcatgtgca atccgactgc aacgaccatt cctgatgcgc cccagtggtg
    14281 cagccctcgg acgaaccagc caaaggtcac ctggtgttcg atgaattgca cgctgtcata
    14341 ggcatggtcc ggcgtcggcg catagtagac ggcgagaaac atacctgtga tcgcctggag
    14401 gataaacagg aagagtgtgg ccgagccgaa gacatagatc cagcttgcgc cgcctggaat
    14461 cggctcatcg agcagcgtgc gttcgacgga cttcagattc aaccggctat cgagccactg
    14521 atagagtcgg gaagacatgg tctagctagg agtaaggagt gagtagtgag gaggtccgaa
    14581 cgatcataaa tctcctcact acttactctt cgctcctccc tttccgtgaa gagagatctg
    14641 atgtcggatc agaccgctaa gcatcttgtc gatctcgttc attcgatcgt caagaatgcg
    14701 ccaagattcg tcgtgcagat accctaaccg tttgacgatt tcaagctgag tgtccaactc
    14761 gctcagtgat ccttgggcca tgtggagaaa tctcgcaaaa tcccgcttgc tatttctcgc
    14821 agcaccctcg gcaatattcg ttgcaatgct tgtcgcggcg cgtctcatct ggtcagtgat
    14881 ggagtatcgc tcttccagtg gaaatttctc agtggcctga ttgatcatga gggatagctc
    14941 aacggcctgt ttccaaacgt tcagcctctt gtgcggcttt tccattgctt acccaatctg
    15001 tttctcctcc cctttcattg ctggctcctc actcctccct cctgactcct tactcctcac
    15061 tgattactag atttcaacct gatcagagac ccccgacttg aattctttgt acatgaccag
    15121 cagtcgcccc tggtcaacct tggaaggaag ggcatccaga tggcgcggtg cagggccggc
    15181 aagtacctga ccggtcacat cgtagacact gccatggcag ggacatttga acttccgatc
    15241 ggtgacatcc cagtgatagc cacatcctaa atgagtgcac atgggagaaa acactgtcac
    15301 ctgcccgtct gattgcttga cc
//
LOCUS       DATIFR010000071        10229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_236935, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000071 DATIFR010000000
VERSION     DATIFR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236935"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VLD60_03945"
     CDS             complement(<1..661)
                     /locus_tag="VLD60_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSB44137.1"
                     /translation="MFLASVDKPMDRIEINYRALLAVAVVLNSQRDMNGLWEAITAEI
                     TKVIPWARASVTLYDREADGFRFYVMATTMAQVVLLRKTVVPRVGSGMGWAYDHKAVH
                     IRPDLKRDQVFLEDKWYLQEGLERMINLPLLVGDRCLGVLNIGSREPGTPDPGVLEFL
                     TQVAIQIASAIDHVQAYEQIDRLRDQLAKENVYLSEELKLEKDMGSLVGKSPAFQHVL
                     DL"
     gene            complement(1716..2978)
                     /locus_tag="VLD60_03950"
     CDS             complement(1716..2978)
                     /locus_tag="VLD60_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSB44138.1"
                     /translation="MTEGSWKPVVIVGGIVLAAYLTWELRSLILPLTVSGLLAYVSRP
                     LVTGLERHHVPRGLAIGLVLAGFLLACLVIIVGVQAAIPTEHKVIELKVHALYALNER
                     YKALMGLDQSPKTGNRLYHLVHTDLDPLMDHVNEVLGLTPEEHAEFMASHAGPAGIEP
                     GSDQLLSEHRANLQIHNVRGRTLSPDAGTAGRADQALGQGWTKFVRSPLTALGDVLST
                     WIVAPLVFFFLLRDTGEIKRGLLRLVPNRLFEPALAILADLDHAVGDYLRGVSLSCSL
                     LGLTITLFLALIGVPLRWAFAIGLLAAVTNVVPYLGSVVALLAGLAYAFFGEDFHPVL
                     PMVERESLAIWVIVAVGVAELIKNAVYEPLVLGGAVRLHPLVIIIGFVGGTLMFGLVG
                     AILAIPAITVFTVFISSTTRHLKAYGVI"
     gene            complement(2975..3574)
                     /locus_tag="VLD60_03955"
     CDS             complement(2975..3574)
                     /locus_tag="VLD60_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSB44139.1"
                     /translation="MTQNKFNLIGIKKLLLPVKHVDLIRGGGPTRALFGFLPSIAAKK
                     SGQDLADFLKQVPLFEDVGVGDLRRLARLLHERSYRDGEYILEQGKPSAAMFVIRTGA
                     VEILRRSQNSEEVHLATLESPASLGESVLMAAEAVNWFSARAHGPVSIVALGRSDLEA
                     LSHNFPLLANKVLAKLAQITALRLQMLVVELTSEQKDSE"
     gene            complement(3705..6182)
                     /locus_tag="VLD60_03960"
     CDS             complement(3705..6182)
                     /locus_tag="VLD60_03960"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HSB44140.1"
                     /translation="MNENEGLKEVQRRYGCGPAQFHGTDDALYDRHLIFDSVIDPKEA
                     APRDQFEAVARSLRDVCSQRWLLTEKTYQRKNPKRVYYLSMEFLLGRSLVNNALNAGL
                     LEIVRDILMKKGYVDPALIETEPDAGLGNGGLGRLAACFLDSMATLELPGMGYGLRYE
                     YGMFKQSIKDGWQIERADNWMRRPDPWEVARLEEAAEIHLHSSVELHEGELRPVIGRP
                     STLLGIPFDRPVIGYGGKTINTLRLWSAATPEFFDFQQFSRGHFVGALTETLASESIT
                     RVLYPDDSTSQGQRLRLVQEYFLVACSLADLVRRFRRINGDWSAFPEKVAIQLNDTHP
                     AMAVAELMRILLDEAHLSWEQAWELTERTMAYTNHTLLPEALEKWPLSWLEMWVPRLA
                     EIVCEINRRFLGSVRAQFPGDEARLNRVSLIEEGTDPKVRMAHLAIVGSHSTNGVAAI
                     HSELLRTMTVKDLAEMFPKRFNNKTNGVTPRRWLLLCNPAWARAINDAIGDSWITDLS
                     QLARLKPLANDSSFQEAFRKAKREAKTQFVDWLRASTGEVVDPDTMFDSHIKRIHEYK
                     RQLLNALHIVVLYNRLRENPRLDFAPRTFFFAGKAAPAYALAKLVIKFINNLAGTIKG
                     DPAVRGRLKVVFVPDYSVSVAERLIPASDVSEQISTAGYEASGTSNMKFMMNGALTIG
                     TRDGATIEIAQEAGEDNVFLFGLTAEQVANSRGWYNPQWHYENEPETRRALDLIFSDH
                     SSLYEPGLFNPLREALLTHGDYYMHLADLTSYSEAQERLGKLYADPHGWTHKAIVNVA
                     SSGRFSSDRTIAEYAADIWNVKPCPVL"
     gene            complement(6179..8269)
                     /gene="glgX"
                     /locus_tag="VLD60_03965"
     CDS             complement(6179..8269)
                     /gene="glgX"
                     /locus_tag="VLD60_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSB44141.1"
                     /translation="MALHPRTQPGRSSPLGSTVSPEGVNFSVFSKSSTRIELLFFERV
                     DDSKPSTVIPLDPITNRTYHYWHVFVPDIQPGQIYGYRAHGSFAPGKGLWYDPDKILI
                     DPYGKAVAVPERYSRVSASAPGDNCATAMKSVVVDPSLYDWEGDIPRKRPFAQTVIYE
                     MHVRGFTRHPSSGLPAETRGTYRGLIDKIPYLKDLGVTAVELLPVFQFDEQDAPKGLV
                     NYWGYSPVSFFAPHQGYSSRKDHLGPTDEFRDMVKALHRAGIEILLDVVFNHTAEGNH
                     TGPTLCYRGLENAAYYMLQEDRSRYADYTGTGNTVNANQPIVRRLIMDSLHYWVEEMH
                     VDGFRFDLASILSRDEDGQPLANPPILWDIESGPALAGTKLIAEAWDAAGLYQVGSFV
                     GDSWREWNGQFRDEVRSFMKGDRGTVTCLARRLLGSPDIYGHQEREPEKSINFVTCHD
                     GFTLNDLVSYNHKHNEANGEGNRDGSENNFSWNCGVEGPTDNPDVERVRTRQVKNFLA
                     LTLLAVGAAMVLMGDEVRRTQRGNNNAYCQDNEISWFDWTRLEKHADIHRFTRLLLRL
                     RSMRDLTRREGDLTLNEILGQARISWHGVKLNQPDWSEESHSLALTFNSLEGCFLVHV
                     IMNAYWESLAFELPPFEGNSHEGWRRAIDTALAPPDDISEGPDDAPRVTDGNYLTQPR
                     SVVLLFAFRSMEEL"
     gene            complement(8454..8549)
                     /locus_tag="VLD60_03970"
     CDS             complement(8454..8549)
                     /locus_tag="VLD60_03970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HSB44142.1"
                     /translation="MKKLTLQYNKTRQAAITKELMDIVGGAEALR"
     gene            8618..>10229
                     /locus_tag="VLD60_03975"
     CDS             8618..>10229
                     /locus_tag="VLD60_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSB44143.1"
                     /translation="MGQHYLRMLFSPKSVAVIGASERTNAVGEIVFRNMLESGYTGLL
                     YPVNPAHQEVQGRPAYATIEAIGAPVELAVICTEAETVPDLIEACGKHGVRAAVVLSA
                     GFSEVGAHGAALEHAMLANAKNYGVRIIGPNCLGMMRPAIGLNATFFNGHIKSGDLAL
                     VSQSGALCTAILDWAPAHDIGFSSVVSMGAASDVDFGEILDYLASDVRTSSILIYVEG
                     IRKARGFMSALRAAARSKPVFVIKVGRHDAGSRAVMSHTGALVGSDDVFDAALRRAGV
                     VRVESIGGLFSAAKSLASHHRYSGNRLAIITNGGGPGVMAIDRAVDLGVSVASLSAAT
                     IERLNQVLPATWSHNNPVDIIGDATPQRYHDAVAICMEDPGVDGALVMLTPQAMTQPL
                     EVATLLVEAARKFSKPLLTSWMGHSQIIEGRGLFAQAGIASFSTPEAAVEAYSYITAY
                     YRNQRLLAQVPPPLSHHDVPDVEGARMLIESVLAERRKVLSEMESKALLSAFHIPVAK
                     TVVVRAPNEGLLMAEEFGFPVAMKINSPD"
BASE COUNT         2103 a         2991 c         2943 g         2192 t
ORIGIN      
        1 caaggtctaa cacatgctga aatgcagggc tcttccccac caatgagccc atgtccttct
       61 cgagcttcag ttcttcgctg agatagacgt tttccttggc aagttggtct cgcagtcgat
      121 cgatctgctc gtacgcttgg acatgatcga tcgcagaggc aatctgtatc gccacttgcg
      181 tcaagaactc gagaaccccg ggatcgggag ttcccggctc tctactgccg atattgagaa
      241 cgccaagaca cctgtcgcca acaagaagcg gcagattgat cattcgttcg agaccttctt
      301 ggaggtacca cttgtcttcc aggaagactt gatcccgctt caggtcagga cggatgtgta
      361 cggctttatg atcgtaggcc caacccatgc cgcttccgac acgtggaacc accgttttgc
      421 gtaacagcac gacttgtgcc atcgtcgttg ccatgacata aaacctgaac ccgtcggcct
      481 cgcgatcgta tagggtgaca gacgcacgtg cccaagggat caccttcgtg atctccgcag
      541 taatcgcttc ccacaggccg ttcatatccc gctgcgaatt gagcaccaca gcgacagcca
      601 agagggctcg gtaatttatc tcaatccggt ccatcggttt gtcaacgctc gctaagaaca
      661 tgaattgaat cacacagcct gcgctgatgt ctatgacgtc ctcctagaaa acttgatcat
      721 ccaatacagg actattctgc tcgttcggaa tcacgttcgg acaatcgcat atttgagaag
      781 ggagtctacg gattccaccg cgagcaagca gtgctgaatg accttccctc tgaaaactag
      841 accattagct agaaagctcg actaggtgtc gaccagccct gcgaatatag gttaaacacg
      901 atctgaagaa ttggcctgag taaccacgtt cagacgattg gattttatag gcgggagcaa
      961 atggaaatca tcgcaacaga taacacttga aagcgaacgc aatgaggtgc cactagtggc
     1021 acctgactct gtaactcatt gaatttccat gcaaccaaat gtcacttttt gcactgcctt
     1081 tttctcttaa cttcttgatt ttgcttagct tgatgatgcc ctgaaaaggc tggtgtcgac
     1141 agttcgattc tgtccctcgg caccacttca atcacttaca caattcaaaa ttagttctag
     1201 ctaggcaagt ggcactcaat tagcatctgt gtcagattat tagtgtcttt cgttggctcc
     1261 cctaacatct tccttctctc atccattctc aggatcggcc aggcgcagcc tggaacatcg
     1321 agacctcatt cagtctcaat aaccttgagg cccttctgaa cgtcgcgcgt gaggccaggg
     1381 agtggattgc cgctcacgcc ttgaagcgtt gagatgtttt catgggaagc catccggcca
     1441 tccggaggct tctcaagctt ttttgtttgt cggtctgtcg gtaccagtgc agtagaaaga
     1501 atctctaact agtggtcaca tcaccaaatt tacaggagta gcgttctaac aaactgcagg
     1561 tcacttttct cagtgggatt cagagacgct gctggatacc cgtcattctg tgccagggtt
     1621 ttggcatggt gtggcgggca agattgccac gattgcttgg ggaaaaacgc tgtaggaggc
     1681 aacccgctcg gagcgaacgg cctgtgaaag tgctttcaaa taaccccgta ggcctttaag
     1741 tgtctggtcg tgctcgagat gaagactgtg aagacggtga tggcgggaat cgcaagaatg
     1801 gctccaacaa gaccaaacat gagagtgccg cccacgaacc cgataatgat cacaagggga
     1861 tggagccgca ccgcgcctcc gagtacgagc ggctcgtaga ctgcgttctt gatcagctcg
     1921 gccacgccca cggccacaat gacccagatc gccaagctct ccctctccac catgggcaag
     1981 accggatgga agtcctcccc gaagaaggca tacgcgagtc cagccagcaa ggcgacaacg
     2041 gatcccaggt aaggaaccac gttggtcact gcggccaata aaccgatggc gaacgcccag
     2101 cggaggggca cgccgatgag ggcaaggaac agcgtaatgg tgaggccgag caaggagcac
     2161 gacaaggaga ccccccgcaa gtaatcacct accgcatggt cgagatccgc caggatggcg
     2221 agcgccggct caaagagccg attcgggacc agtctgagaa ggccgcgttt gatctcgccc
     2281 gtatcgcgca gcagaaagaa gaacaccaga ggggcgacga tccaggtgga gagcacatcg
     2341 ccgagcgctg taagaggcga tcgcacaaac ttggtccacc cttggccgag ggcctggtcc
     2401 gcacggcccg ccgtgccggc atcgggggac aatgtccggc cgcgcacgtt gtgtatctga
     2461 agattagcgc ggtgctcact gagcagttgg tctgagccgg gctcgatgcc agccggtccc
     2521 gcatgggacg ccataaactc agcatgctct tcgggcgtca agccgaggac ctcgttcacg
     2581 tgatccatca gcggatcgag gtcagtgtgc accagatggt agaggcggtt tccggtcttc
     2641 ggcgactgat cgaggcccat taaagccttg tagcgctcgt tgagggcata gagcgcgtgg
     2701 accttcagct cgatgacttt gtgttccgtg ggaatggcgg cctgtacacc cacaatgatg
     2761 accaggcagg ccaacaggaa gccggccagc accagtccaa ttgccaggcc acgtggcaca
     2821 tgatgacgct ccaaaccggt gacaagagga cgactgacgt acgccagcag gccgctgacc
     2881 gtaaggggaa gaatcagact ccgcagctcc caggtcagat aggcggcgag cactatgccc
     2941 cccacaatca caaccggttt ccatgaacct tcggtcactc cgaatccttt tgctcgctgg
     3001 tcagctccac aaccagcatt tgaagtctca gggcagtgat ctgagccagc ttcgcaagaa
     3061 ccttgttcgc caggagtgga aagttgtggc tgagtgcctc caggtcagaa cgtccgagcg
     3121 caacgatgga gactggacca tgagcccgcg ctgaaaacca gttcacggcc tcggctgcca
     3181 tgagtacgga ttcccccaag gaggcaggcg actccaaggt agccaagtgt acttcctcgc
     3241 tgttctggct gcgcctcagg atctcaacgg caccggtgcg gatgacaaac atggctgcgc
     3301 ttggtttccc ttgctcgagg atgtactcgc catcccggta acttcgttcg tggaggagcc
     3361 gggccagtcg cctgagatcc ccaacgccga cgtcctcgaa gagcgggacc tgcttaagga
     3421 agtccgcaag gtcctggccg ctcttcttgg cagcgatgct tggaagaaag ccgaacagcg
     3481 cgcgagttgg accgccgccc cgtatcaggt ccacatgctt gaccggtaac aggagcttct
     3541 tgataccgat cagattgaat ttgttctgcg tcattgtggt caccgaagtg gcggcgttct
     3601 cagaagcgtg tgattcagat cctcaccgac tcacaacctg tcagggagca ccttacacca
     3661 tgcacgagtg cctcgggcgt tagttgaatg catcgccgtt gccattacag aaccgggcac
     3721 ggcttcacat tccaaatatc agccgcatac tcggcaatgg tccggtcgct ggagaacctc
     3781 cccgagctcg cgacattgac gatagctttg tgagtccagc cgtgcgggtc agcgtaaagt
     3841 ttccccaatc gttcctgcgc ctcgctgtac gatgtgagat cagccagatg catgtaatag
     3901 tcgccgtgcg tcagcagggc ctcgcgaaga ggattgaaga gtcccggctc atacaggctg
     3961 gagtgatctg aaaagatcag atccagcgcc cggcgtgttt ccggttcgtt ctcgtaatgc
     4021 cactgcgggt tgtaccatcc ccggctgttc gcgacctgtt ccgctgtcag gccgaagaga
     4081 aagacattgt cctcgccggc ctcttgagcg atttcgatgg tcgctccatc gcgtgtgccg
     4141 atcgtcaatg cgccattcat catgaatttc atattgctcg tcccgctggc ttcataaccc
     4201 gccgtcgaga tctgttccga aacatcgctg gccggaatca gccgttccgc caccgagacg
     4261 gaataatccg gcacaaagac aactttcagc cgcccccgca cggctggatc tcccttgatg
     4321 gttccggcaa gattgttgat aaacttgatg acgagcttgg cgagcgcgta ggccggagcc
     4381 gccttgccgg cgaagaagaa cgttcggggc gcgaaatcga ggcgaggatt ctcgcgcaga
     4441 cggttgtaga gcacgacgat gtgtaaggca ttgagcagtt gcctcttata ttcatggatg
     4501 cgcttgatgt ggctgtcgaa catggtgtcg ggatcgacta cttcaccggt cgacgctctg
     4561 agccaatcca cgaactgcgt cttggcttcg cgtttggcct tgcgaaacgc ctcctgaaag
     4621 ctgctgtcgt tggcgagcgg cttcaaccgg gccagttggc tcaggtctgt gatccagcta
     4681 tcgccgatcg cgtcattgat cgctcgtgcc catgcgggat tgcagagcag caaccagcga
     4741 cgtggggtga ccccgttcgt cttgttgttg aaacgtttcg gaaacatctc ggcgaggtcc
     4801 ttcacagtca ttgtccgcag aagttctgag tgaatggccg ccacaccgtt ggtgctgtgc
     4861 gaaccgacga tcgcgagatg cgccatgcgc accttcggat ccgtgccttc ctcgatgaga
     4921 ctcacgcggt tcagacgtgc ttcgtcgccg gggaactggg cacggaccga gccgaggaag
     4981 cgccggttga tctcgcaaac gatctcggcc agccgcggga cccacatttc gagccaactg
     5041 agcggccatt tctcaagcgc ttccggcaac agtgtatggt tcgtgtaggc catcgtccgt
     5101 tccgtgagtt cccaggcttg ctcccagctg aggtgcgctt cgtcgagcag gatccgcatg
     5161 agctcagcca cggccattgc cgggtgcgtg tcgttcaatt gaatggcgac cttctccggg
     5221 aaggcgctcc agtcaccatt gatgcgtcgg aagcggcgga ccagatcggc gagcgagcac
     5281 gcgaccagga aatactcctg cacaaggcgc agcctctgcc cctgactggt cgagtcgtcc
     5341 gggtacagca cccgggtgat ggattccgat gccaacgttt cggtcagcgc gccaacgaag
     5401 tgcccccggc tgaattgctg aaaatcgaag aactccggcg tggcggcgga ccagagccgc
     5461 agcgtattga tggtcttccc gccgtagccg atcaccggcc ggtcgaacgg gatgccgagc
     5521 aaggtcgagg gccgtccgat gaccggccgg agttcaccct cgtggagctc gactgacgag
     5581 tgcaaatgga tttcagcggc ttcttccagc cgggccacct cccatggatc ggggcgccgc
     5641 atccagttgt cggcccgttc gatttgccag ccgtccttga tgctttgctt gaacatgccg
     5701 tattcatagc gtagaccgta ccccatgcct ggcagctcga gcgtggccat cgaatccaga
     5761 aagcaggcgg ccaaacggcc gagtccgccg ttcccgagcc cggcgtcggg ctctgtctcg
     5821 atcagagccg ggtcgacata gccttttttc atgaggatgt cgcggacgat ctccagcagc
     5881 ccggcattaa gcgcattgtt gacgagcgag cggcccagga ggaattccat ggagaggtag
     5941 tacacccgct tcggattctt gcgctggtac gtcttctccg tcagcagcca gcgctgtgaa
     6001 cagacgtctc gcagcgaccg tgccacggct tcgaactggt cgcggggcgc ggcctccttc
     6061 ggatcgatca cactgtcgaa tatcagatgc cggtcgtaga gcgcatcatc agtcccgtgg
     6121 aactgggccg gtccacatcc atagcgcctc tgaacttcct tcaagccttc gttctcgttc
     6181 ataactcctc catcgagcga aacgcgaaca acagtacaac ggagcggggt tgagtcagat
     6241 aattcccatc cgtgacccgc ggtgcatcat ctggtccctc ggaaatgtcg tcgggcggtg
     6301 ccagcgcggt gtcgatcgcg cgacgccatc cctcgtgcga gttcccctcg aatggcggca
     6361 gctcaaaggc cagcgactcc cagtacgcat tcatgatcac atgaacaagg aaacacccct
     6421 ccagggagtt gaaggttaga gcgaggctgt gcgactcctc gctccagtcg ggctgattca
     6481 gtttcacccc atgccagctg attcgtgctt gcccaaggat ctcgttcagc gtcaggtccc
     6541 cctctcttcg cgtcagatct ctcatggagc ggaggcggag caggagacgg gtgaaccgat
     6601 ggatgtcggc gtgcttctcc agcctcgtcc agtcaaacca gctgatctcg ttgtcctgac
     6661 agtaggcatt attattcccg cgctgagtcc gtcggacctc gtcgcccatg aggaccatcg
     6721 ctgcaccgac ggccagcagt gtcaacgcca ggaagttttt cacctgccgg gtgcgcacgc
     6781 gctcgacgtc cgggttgtca gttggacctt ccaccccgca attccagctg aagttgttct
     6841 cgcttccgtc ccggttgccc tctccgttcg cctcattgtg tttgtggttg tacgacacca
     6901 ggtcattcaa cgtgaacccg tcatgacagg tgacgaaatt gatgctcttc tccggctccc
     6961 gctcctgatg gccatagata tccggactgc ccagcaggcg gcgagccagg caggtgactg
     7021 tgccccggtc ccccttcatg aagctgcgca cctcatcccg gaattggccg ttccactctc
     7081 tccaactatc cccgacaaac gagcccacct ggtacagccc ggccgcatcc caggcttccg
     7141 cgatgagttt cgtcccggcc agggctggac ccgattcgat gtcccagaga atggggggat
     7201 ttgccagcgg ctggccgtcc tcatcccgag ataggatcga ggcgagatcg aaacgaaagc
     7261 cgtcgacgtg catctcttca acccagtaat gaaggctgtc cataatcaaa cggcgaacga
     7321 tcggctgatt cgcgttcacg gtgttgccgg tgccggtata gtcggcataa cgggatcggt
     7381 cctcctgcaa catgtaatag gccgcgtttt ccagcccgcg atagcagagc gtcgggccgg
     7441 tgtggtttcc ctccgccgta tggttgaaga cgacatcgag gaggatctcg atgccggccc
     7501 gatggagggc cttgaccata tcacgaaact catccgtcgg gcccaagtga tccttgcggg
     7561 agctataccc ctggtgcggc gcgaagaacg agaccggact gtagccccaa tagttcacca
     7621 aacctttcgg ggcgtcctgt tcgtcgaact ggaagaccgg cagcaactcc actgcggtca
     7681 cgccgagatc tttgagatac ggaattttgt cgatcaatcc gcgataggtg cctcgtgtct
     7741 cggccggcag ccccgaactg ggatgacggg taaatccccg cacgtgcatc tcatagatca
     7801 cggtttgggc gaacggccgc ttccggggaa tatcgccttc ccagtcatac aggctaggat
     7861 caaccaccac gctcttcatc gcggtcgcgc aattgtcgcc gggtgcggag gcgctgacgc
     7921 gactgtagcg ctcaggcacg gcgactgcct tgccgtacgg gtcgatcagg attttgtccg
     7981 ggtcgtacca aaggcctttt cccggtgcga atgaaccgtg tgcacggtag ccgtagatct
     8041 ggccgggctg aatatcgggc acaaaaacat gccaataatg gtacgtgcgg ttcgtgatcg
     8101 gatcgagagg aatgactgtg ctcggcttcg aatcgtcgac cctctcgaag aacagcaatt
     8161 caatgcgtgt gctgcttttc gagaacacgc tgaagttgac tccctcagga gaaactgtcg
     8221 agccgagcgg ggagctcctg cccggctggg ttcttgggtg caatgccatc gggtcgcctt
     8281 cctgcggcgg cttccagcgt gttatctatt gtgaggccac ttcccgtgcc tgacttccgc
     8341 atatcatcgc ggtgcttggc aacatcctct gtgtgctcca agcctttgtc gataactctg
     8401 cacctgaggc atcgaccggt caagtctact tcacgacgcg tagcttcagg tgattaccgc
     8461 agggcttcgg caccaccaac gatgtccatc aattccttcg taatggcggc ttggcgggtc
     8521 ttgttgtatt gcagcgtcag ttttttgatg aaagatgcgg atagagcatc ccgccgtgta
     8581 gtgttatatt cacctcaacc aaccacgagt gcaagccatg ggacagcatt acctacgcat
     8641 gcttttttcg cccaagtcgg ttgccgtcat tggtgccagc gaacgtacca atgccgtggg
     8701 cgagatcgtc ttccggaaca tgctggagag cgggtatacg ggtttgctct atccggtcaa
     8761 tccggcgcat caggaggtgc aaggccggcc ggcctatgcc acgatcgaag ccatcggagc
     8821 cccggtcgag ttggccgtga tctgcaccga ggccgagacg gtgcccgatc ttatcgaggc
     8881 ctgcggcaaa catggtgtgc gtgctgcggt ggtcctgtcc gcaggattca gcgaagtcgg
     8941 tgcccatggc gctgcgctcg aacacgccat gctggccaat gccaaaaatt atggtgtgcg
     9001 cattatcggc cccaattgtt tgggcatgat gcgtccggcg atcgggctca acgccacgtt
     9061 ttttaacgga cacatcaagt ccggcgacct tgccctggtt tctcagtcgg gggcgctgtg
     9121 cacggccatt cttgactggg cgccagccca tgacatcggt ttttccagtg tggtgtcaat
     9181 gggcgccgca tccgacgtgg attttggtga gattctcgat tacctcgcga gcgacgtgcg
     9241 gacgagcagt attctgatct acgtcgaagg catccgcaaa gcgcgtggct tcatgagtgc
     9301 gttgcgggcg gccgcacgta gtaaaccagt ctttgtgatt aaggtgggcc gtcatgatgc
     9361 gggatcgcga gcggtgatgt cgcacactgg cgcgctggtt ggttcggatg atgtgttcga
     9421 tgcggcgctg cgccgcgccg gcgtggtccg tgtggaatcg atcggcgggc tgttttctgc
     9481 cgcgaagtca ctcgcctcgc accaccggta cagcggcaac cgtctggcca tcattaccaa
     9541 tggcggcgga cccggcgtga tggccatcga ccgcgcggtg gatctgggcg tgagcgtggc
     9601 cagtctgtcc gctgcaacca tcgagaggct gaaccaggtg ttgcctgcga cctggtcgca
     9661 caacaacccg gtcgacatca tcggcgacgc cacgccgcag cgctatcacg acgcagtcgc
     9721 catttgcatg gaagaccccg gcgtggatgg tgcgctggtg atgctcaccc cccaggcgat
     9781 gacgcagccg ctggaagtcg ccacattgct ggtcgaggcg gcaagaaaat tcagcaagcc
     9841 gctgctcacc agttggatgg gtcattctca gattatagag ggacgaggcc tgttcgctca
     9901 ggccggcatt gcctcattca gcacccctga agcggcagtt gaggcctatt cctacatcac
     9961 cgcgtattac cgcaatcaac gtctgttggc gcaagtcccg ccgcccttat cgcaccatga
    10021 tgttccggat gttgagggtg cgcgcatgct gattgagtct gtcctggcgg aacggcgcaa
    10081 ggtgttgtct gagatggaat cgaaggcgct cctgtcggcc ttccatattc ccgtggccaa
    10141 aacggtggta gtccgtgctc ccaacgaggg gctgctgatg gcagaggagt tcgggttccc
    10201 ggtggcgatg aaaatcaatt cgccggaca
//
LOCUS       DATIFR010000072         9664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1576843, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000072 DATIFR010000000
VERSION     DATIFR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576843"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VLD60_03980"
     CDS             <1..1425
                     /locus_tag="VLD60_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSB44144.1"
                     /translation="KAVITVPAYFNDSQRQATKDAGMIAGLEVLRIINEPTAASLAYG
                     LQERTQGTIAVYDFGGGTFDISILKLKNGIFEVLATNGDTHLGGDDLDRTLADRFVGE
                     IRNAHGIDLSAYPDHMQAVRLEAERAKIRLSDELKTQVSVELPENRGRYTRELTRDQL
                     QSLTMEIIERTMAPCRLALKDAGLTPNDIDEIVLVGGSTRMPLVRQRVQDLFGKTPHC
                     ELNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMGGVMSSLIRRNTTIPAS
                     AKEMFTTYVDGQTGVDIHILQGERELAKDNRSLARFRLKVPPLPAGVPRIEVTFLIDA
                     NGILNVKASDIRTGQSQSIEVKPSYGLSDNEVERMIEDSFKFAADDVNARKLIEARLD
                     AGALITTSEKSLAEGGHLIPQEDVAAIRAALSVLATAKDGNDPRAIRARMAELEESAK
                     GLTVAMLDDSLKRGLQGKKVSEVT"
     gene            1448..1660
                     /gene="iscX"
                     /locus_tag="VLD60_03985"
     CDS             1448..1660
                     /gene="iscX"
                     /locus_tag="VLD60_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HSB44145.1"
                     /translation="MDLKWQDAEDIAIRLVEEHPETDPLMVRFTDMHAWIVALPDFRD
                     DPKKSNEKILEAIQMAWHEEYKDSKS"
     gene            complement(1720..4107)
                     /locus_tag="VLD60_03990"
     CDS             complement(1720..4107)
                     /locus_tag="VLD60_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HSB44146.1"
                     /translation="MTTIDRPVQLSDMPRQPPPRRPWRWWQVALIAVAVIGMLGVLAV
                     AGVLWHYAQDLPDLNQLQNYQPSLVTQVYSGDNQLIGQFFLERRILTPLSQIPEKLRG
                     AVIAVEDVRFFEHPGLDYIGILRAAWTNLRRGGKVEGASTITQQLARSLFLSSERTID
                     RKIRELVLAYKMELVSTKEQILETYLNQIYFGQGAYGVASAAQSYFGKDLSALTVAEA
                     AFLAGLPKSPNNFSPFKNYERAKKRQEHVLARMEEAGFLTSAEREQAAAEPLSFRRPG
                     SDQTAPYFVEYVRQLLMAKYGEAMVYKGGLKVTTTLNLEMQRAAEAAFEVGLRELDKR
                     QGWRGPLRTVDPASVTPTNLPVVSTVQPVAVGDYREAVVTKVGKDFYMAQVGSTPAKL
                     LFDDMAWAKRRLTGPDPTKDVVVNQNLKHILKPGEVIEVRVKKIEKDTVYVQLEQTPL
                     VEGGLIALEPDKGAIRAMVGGYDFNRSEYNRAVQAHRQPGSAFKPIIYATAVNQGMSP
                     ASVVLDAPVVYEQVEEEKTWKPENYGKKFHGMVSLRDALAQSHNLATVRLLDKVGVKS
                     VIEFARQVGITSPLAADLSLGLGSSSVGLMELTSAYSVFLNQGSRIEPYAIFSVIDNS
                     GQVLEHAELQAQDVISKETAYLITNMMEDVVQKGTGQAAKSLGRPIAGKTGTTNEFIN
                     AWFIGGTPNLVTGVYVGFDDRRSLGETESGAHAALPIWINFMREALKPLPVVPFAIPE
                     GITFVKVDPATGLLNSEQEDQPGNVEIFTRGSEPTVAAQRRVDPIDFYKLDQPSE"
     gene            4314..6170
                     /gene="typA"
                     /locus_tag="VLD60_03995"
     CDS             4314..6170
                     /gene="typA"
                     /locus_tag="VLD60_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSB44147.1"
                     /translation="MSQLRAPNNRRNDIRNIAIIAHVDHGKTTLVDAVLRQTHVHRKI
                     EDMGERIMDSMDQERERGITIRAKNASVIYNGVKINIVDTPGHADFGGEVERTLRMVD
                     GVLILIDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVNRTFDLFVHL
                     GATDEQLDFPIVYASAIKGQATLDVNKPGTDITPLLDTVLEKIPPPAINPNAPLQILV
                     LSLIQDTYKGKMGVGKIQSGSIARRQNVVVLGKDGTQIPGKVSDLAVYSGLERTDMER
                     AEAGEIIAVAGLDEVSIGDTIADAEQPVALPRVTVDEPTVQMTFSVNNSPFAGREGKY
                     LTSRHLRDRLFKELETNVSLRAQETDSADKFLVAGRGELHLGVLIEQMRREGYELQVS
                     QPEVIVHREGDKVLEPYEELTIQVPETYQGTVIEELGKRRGELRHMKLVHSDAGPSEM
                     HLEYHIPTRGIMGLKNLLVAKTRGTIIMHHVFVAYEPVEERDLAVASHGSLVAYEDGT
                     STGYAIFMTQERGTMFIGPGVEVYRGMVVGENSRDEDMDVNVCKEKHLSNMRASGTDE
                     ALVLTPPREMSLEFALEYIGPDELLEVTPQNLRMRKRLLNPDDRRKARKSGK"
     gene            6187..6936
                     /locus_tag="VLD60_04000"
     CDS             6187..6936
                     /locus_tag="VLD60_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44148.1"
                     /translation="MRIKKKSPKPSSKRPPLYFIGYRGTAPQPDELKTWYDLEYGGAL
                     TVKPEADAPESWHVSHGPWTAHVVIPLPASHTAGFKEQLAWEHELIGAVAPSVMPPRD
                     MPDAILFAARISRGLTLLTQGTAYDITTQQYLNPSDWKDRGLTAFVADDHVMIAQEDQ
                     AKPEEVWWYTLGLSKFGIDELETFQPRGLSDRPAKELLIESAHEMIRIGQSPKVGMAL
                     RLPILGSTIRIVNHRTAAPIGRMLSFREVRS"
     gene            7221..7691
                     /locus_tag="VLD60_04005"
     CDS             7221..7691
                     /locus_tag="VLD60_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSB44149.1"
                     /translation="MSDVAPEIKVGDTAPDFNLKDQDQKDVKLSDYKGKKNVVLAFYP
                     LDWSPVCQGENKCLTDDFPKFQSANAELFGISCDSFFSHKAWADSMDLKHRLLSDVHR
                     TTAKAYGLYFEPLNCSKRATVIVDKNGKVAYVKVQEIKVAREDKEILAALAKLN"
     gene            7759..8091
                     /locus_tag="VLD60_04010"
     CDS             7759..8091
                     /locus_tag="VLD60_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSB44150.1"
                     /translation="MSGTVQDVRDENYKEFTDSTAAVVAYGLATCEPCKTYDPILEET
                     AAKFPQIKVGKAKMHVPGRCREIKKTHTFETYPTTHFFANGKLLLTREGVVEPAELAA
                     LISDHLLK"
     gene            complement(8262..8741)
                     /locus_tag="VLD60_04015"
     CDS             complement(8262..8741)
                     /locus_tag="VLD60_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB44151.1"
                     /translation="MTRPAISAMIIALQVFAGSVVLAGQDEDAVQALLIDSAKATSEF
                     SRTRDPQSVLKAYAKDYVGIQDGESETRETIEKWLSEYGAELDKGNPTRFLGEVFGIK
                     VRVSGALAWATYDYVFKLIANGEIQTEDRGLCTSILRKEGSTWLIQHEHCSKPRPAK"
     gene            complement(8786..9535)
                     /locus_tag="VLD60_04020"
     CDS             complement(8786..9535)
                     /locus_tag="VLD60_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSB44152.1"
                     /translation="MPPVLLTKRIEFAAAHRYIKPEWDEAKNRAVFGPCYNPPAHGHN
                     YMLEVTVSGEVDPKTGMVINLFDLKRVLLTIIEEFDHKNFNLDMPYFNDRIPTSENIA
                     RLLWTKLEPQQDIGTLHTVRLYEDEDLYADITAAGGLDVASVTRRYSFTAVQEGNQGH
                     VWDCFVTVHGPIDSVTGMVTDIGALDRLAQERVVKAFDRQVLERVLGQPVTGNALARH
                     VWGRLAGSVNGGALQLVRLVQTRDLSFEYSG"
BASE COUNT         2168 a         2954 c         2665 g         1877 t
ORIGIN      
        1 aaagccgtca tcacggtgcc tgcctacttc aacgacagcc agcggcaagc cacgaaggac
       61 gccggcatga tcgccggcct cgaagttctg cgtatcatca acgaaccgac cgccgcgtcg
      121 ctggcctatg ggctccaaga acggacccag ggcacgatcg ccgtctacga cttcggcggt
      181 ggcacgttcg atatttccat tctcaagctc aagaacggca tctttgaagt gctcgccacc
      241 aacggcgaca cacatctcgg tggcgatgat ttggatcgga cgcttgccga ccgcttcgtc
      301 ggagaaattc gaaacgccca cggcatcgac ctcagcgcgt accccgacca catgcaggcc
      361 gttcgcctgg aagcggaacg agccaagatc cgcctgtccg acgaactgaa gacgcaggtg
      421 tcggtcgaac ttccggagaa caggggacgc tatactagag agctcacgag agaccaactc
      481 caatccctga ccatggagat catcgaacgg accatggcgc cttgccgact ggccttgaaa
      541 gatgcggggc tgacccccaa tgatatcgac gaaattgtgc tcgtcggcgg ctccacccgt
      601 atgccgctcg ttcggcaacg tgttcaggat ttgttcggga agaccccgca ttgcgagctc
      661 aacccggacg aagtggtcgc cctcggcgca gccgtgcagg ccgatattct gagcggcggc
      721 acgaccgaca tgctcttgct cgatgtcacc cccctctcgc ttggcatcga aacgatgggc
      781 ggcgtgatga gtagcttgat caggcgaaat acaacgatcc cggcaagtgc gaaggagatg
      841 ttcaccactt atgtcgacgg ccaaaccggt gtggacatcc acattctcca aggcgagcgc
      901 gagctggcaa aagacaatcg gagtctggcc agatttcgac tcaaagttcc tccacttccg
      961 gccggcgttc cccgcatcga agtgacattc ctcatcgacg ccaatggaat cttgaacgtg
     1021 aaggccagtg atatacggac cggacaaagc cagtccatcg aggttaaacc ctcctacggt
     1081 ttatccgaca acgaagtgga gcggatgatc gaggattcgt tcaagtttgc cgcggacgac
     1141 gtcaacgcca gaaaactgat cgaagcgcgg ctcgatgccg gtgcgttgat cacgacatcc
     1201 gagaagtccc tggcagaagg cggccatctg atcccacagg aagacgtcgc ggccattcga
     1261 gccgcgttgt ccgttctggc taccgcgaag gacggcaacg acccacgcgc gattcgcgcg
     1321 cgcatggccg agcttgagga atcggcaaag ggcttaacgg tcgcgatgct ggatgattcg
     1381 ctcaaacggg gacttcaggg gaagaaggtg tcagaagtca cctagaccag ggaagcgagg
     1441 aatcggcatg gatttgaaat ggcaagatgc ggaagatatc gccattcgac tggttgaaga
     1501 gcacccggaa acggatccgc tgatggttcg ttttaccgat atgcacgcct ggatcgtcgc
     1561 gttgcctgat tttcgggatg accccaaaaa atcgaacgag aagatcttgg aagcaattca
     1621 gatggcctgg cacgaggagt acaaagactc caagtcctga ccgaataggc aaatcaggcg
     1681 cccgccctgc ggctcatctt ccacgcttac ttctttacat cattctgaag gctggtccag
     1741 cttgtagaaa tcgatcggat cgactcggcg ctgtgccgct acggtcggct cgctccccct
     1801 ggtgaagatc tccacatttc ccggctgatc ctcctgttca gaattcagca gtccggtcgc
     1861 cggatcgact ttgacgaagg tgatgccctc gggaatggca aaaggcacga ccggcaacgg
     1921 tttgagcgcc tctcgcatga agttgatcca gatcggcaga gccgcatggg cccccgactc
     1981 ggtctcgccg agcgaacggc gatcatcaaa cccgacgtag actcccgtca ccaaattcgg
     2041 cgtgccgccg ataaaccagg cattgatgaa ctcattggtg gttcctgttt ttcccgcgat
     2101 gggacgacca agtgatttcg ccgcttggcc cgtccccttt tgcacgacat cttccatcat
     2161 gttggtgatc aggtaggcgg tttccttcga aatgacgtcc tgggcttgca gttcagcatg
     2221 ttcaaggacc tgtccgctat tatcgatgac ggaaaagatg gcatagggct cgatccggct
     2281 cccctggttt aaaaacacgc tatacgcgga ggtcagttcc atcagcccca cggaggagga
     2341 tcccagcccc agcgacagat cagcggcgag cggactcgtg atgccgacct gtcgcgcaaa
     2401 ctcgatcacg ctcttcacgc ccactttgtc gagcaatcga accgtcgcca agttatggga
     2461 ttgcgccaag gcgtcgcgga gactgaccat gccatgaaat tttttcccgt aattctccgg
     2521 cttccacgtt ttctcttcct cgacctgctc atacaccacc ggtgcatcaa gcaccaccga
     2581 tgcgggactc atgccctgat tgactgcggt cgcataaatg atcggcttaa acgccgatcc
     2641 aggctggcgg tgggcttgca ccgcccggtt atactcgctg cgattgaagt catacccgcc
     2701 gaccatggct cgaatcgccc ctttgtccgg ctcaagcgca atcaatcctc cctccacgag
     2761 gggagtctgt tccaattgaa cgtagaccgt gtccttttcg atcttcttga cccggacttc
     2821 gatcacctcg ccgggcttca ggatatgctt cagattctga ttcacgacca catccttggt
     2881 cggatccggc cccgtcaaac gcctcttcgc ccaggccatg tcgtcgaaca gcagcttcgc
     2941 aggcgtacta ccgacctgcg ccatatagaa atccttgccg accttcgtga ccaccgcctc
     3001 tcggtagtcg ccgaccgcga caggctgaac ggtcgacacg accggaagat ttgtcggcgt
     3061 gacggaggcc ggatcgacgg tgcgcaaggg acctcgccag ccttgtcgtt tatccaattc
     3121 ccgcaacccc acctcaaagg ccgcttccgc ggcgcgctgc atctccagat tcaacgtggt
     3181 cgtgactttg aggccgcctt tgtagaccat cgcctcgccg tacttggcca tcagcaactg
     3241 ccggacgtat tccacaaaat acggagctgt ttgatcactg cccggacgtc gaaagctcag
     3301 cggttctgcg gcagcctgct cgcgctcggc ggaggtgaga aaaccggctt cttccatgcg
     3361 cgccaggaca tgttcctggc gcttcttcgc ccgctcatag ttcttgaacg gagaaaagtt
     3421 gttcggtgat ttcgggagtc ctgccaggaa cgccgcttcg gcaaccgtca acgcagaaag
     3481 gtccttccca aaataggact gtgccgccga tgcgacaccg taggcccctt gcccaaagta
     3541 gatctggttc aagtacgttt caagaatctg ttcctttgtc gagaccagct ccatcttgta
     3601 cgccaacacc aactcgcgga ttttccgatc gattgtgcgt tcagacgaga ggaacaatga
     3661 acgcgccagc tgttgggtga tcgtgctggc tccttcgacc ttccctccgc gccggagatt
     3721 cgtccaggct gcgcggagaa tcccgatgta atccaggccg ggatgctcga aaaagcgcac
     3781 gtcctcgacg gcgatcactg cgcctcgcag cttctcgggg atctgtgaga gcggtgtcag
     3841 gatccgccgt tcgagaaaaa actggccgat gagctggttg tcgcccgagt agacttgggt
     3901 cacaaggctc ggttgatagt tttgcagctg attgaggtcg gggagatcct gcgcataatg
     3961 ccacagcacg cccgcgaccg cgagcacccc gagcatgcca atcaccgcca cggcgatgag
     4021 cgcgacctgc caccagcgcc aagggcgccg gggcggcggc tgacgcggca tatcagagag
     4081 ttggactggt cgatcgatcg ttgtcatggg aatcacgcgc ccgcaggggg ctggtgctct
     4141 tcgagaggat acttgttcac cataccgtgg agtcccccaa aactcaagca acttgcaggc
     4201 cagacctcac tcaatctgac ggcttgtagc gctgcaatgc ttcgggtata ctatgcagtc
     4261 gacgcccgac agtcggggcg tctttttttt cgccacgaga agcacaggaa cctatgtcgc
     4321 aactacgcgc ccccaacaac cgccggaacg atatccggaa catcgccatc atcgcacacg
     4381 tcgatcacgg taagacgacg ctcgtcgacg ccgtgcttcg acagactcac gtccaccgca
     4441 agatcgagga tatgggcgaa cgcatcatgg actcgatgga tcaggagcgc gagcgcggga
     4501 ttacgatccg cgccaagaac gccagcgtga tctacaacgg tgtgaagatc aacatcgtcg
     4561 acacaccggg ccatgccgac ttcggcggcg aagtggagcg gacgctccgg atggtcgacg
     4621 gggtcttgat cctgatcgat gcgaaggaag ggcccatgcc gcagacgacc ttcgtgctgc
     4681 gcaaggctct ggcgctcggt cacaaagcca tcgtcgtgat caataagatc gatcgtcccg
     4741 acgcggtcat cgacgacgtg gtcaatcgca cattcgacct gtttgtccat ctgggtgcga
     4801 ccgacgagca gctcgacttt cccatcgtgt acgcctccgc cattaaggga caagccacgc
     4861 tcgacgtaaa caaaccggga acggacatta ccccgctcct cgataccgtc ctcgaaaaga
     4921 ttccgccacc ggccatcaac cctaacgcac cgctccagat tctggtcctc tccctcattc
     4981 aggacaccta caaaggcaag atgggcgtgg ggaagattca gtccggttca atcgcccgca
     5041 gacagaatgt ggtcgtcctc ggaaaagacg gcacacagat ccccggcaaa gtctccgacc
     5101 tcgcggtcta ctccggcctg gaacggacgg atatggaacg ggcggaagcg ggagagatca
     5161 tcgcggtggc cggcctggac gaagtgagca tcggcgacac gatcgcggac gcggaacagc
     5221 cggtcgcatt acctcgcgtc acggtcgacg agccgacggt ccagatgacc ttttcggtca
     5281 acaatagccc gttcgccgga cgggaaggca agtacctgac ctcgcggcat ctacgcgatc
     5341 gactcttcaa agaacttgaa accaacgtgt cgttgcgcgc gcaagaaacg gatagcgcgg
     5401 ataagttcct cgtggccggg cgcggcgaac tccacttggg cgtcttgatc gaacagatgc
     5461 ggcgagaagg atacgaactt caagtatcgc aaccggaagt catcgtccac cgggaagggg
     5521 ataaagtcct cgaaccctac gaggagctga ccatccaagt gccggagacc taccagggca
     5581 ccgtgatcga agaactcggc aaacgccgcg gcgagctgcg ccacatgaaa ctcgtgcact
     5641 ctgacgccgg cccgagcgaa atgcacctgg agtaccatat cccgacgcgc ggtatcatgg
     5701 ggctgaagaa cctcctggtg gccaagactc gcggcaccat catcatgcac catgtgttcg
     5761 ttgcgtacga accggtggag gagcgggacc ttgcagtggc aagccatggt tcgctcgtcg
     5821 cgtacgaaga cggaaccagc accggttatg ccatcttcat gacccaagaa cgaggcacca
     5881 tgttcatcgg ccctggcgtc gaagtctatc gcggcatggt cgtcggcgag aacagccggg
     5941 atgaagacat ggacgtcaac gtctgtaaag aaaaacacct gtccaacatg cgggcgtctg
     6001 gcaccgatga agccttggtc ctcacgccac cgcgagaaat gtcgctcgag tttgcgttgg
     6061 agtacatcgg tcccgacgaa ctgcttgagg tcacaccgca gaacctgcgc atgcggaagc
     6121 ggctcctcaa tccagacgac cggcgcaagg cccgcaaatc cggaaaataa gccctcgagc
     6181 cactcgatga ggatcaagaa gaaatccccg aagccctcat ccaaacgccc acccctgtat
     6241 ttcatcggct atcgcggcac cgccccgcag ccggatgaac tgaaaacctg gtatgacctc
     6301 gagtacgggg gggcgttgac cgtcaagccg gaagcggacg caccggaatc ctggcacgtc
     6361 agccacggtc cttggaccgc ccacgtcgtt attccccttc ctgcctcaca cacggccgga
     6421 ttcaaagaac agctggcctg ggagcatgag ctgatcggcg cggtcgcccc gtcggtgatg
     6481 ccgccgcgtg acatgccgga tgcgattctc ttcgccgcgc gcatctcccg cggcctcact
     6541 ttgctcacgc agggtaccgc ctacgacatc acgacccagc aatacctcaa tccttccgat
     6601 tggaaagatc gggggctcac cgcctttgtc gctgacgatc acgtcatgat tgcccaagag
     6661 gatcaggcca agcccgaaga agtctggtgg tacacccttg gcctctcgaa gttcggcatc
     6721 gatgagctcg aaacgttcca gcccagaggg ttgtcggatc gcccagccaa agaactcctg
     6781 atcgagtccg cgcacgaaat gatccggatc ggccaatccc ccaaagtcgg catggccttg
     6841 cgcctcccga tcctcggctc cacgatccga atcgtcaatc accgcaccgc tgctccaatc
     6901 gggcgcatgc tcagcttccg tgaagtcaga tcgtaagcag cctcgtgata tcaacgagtt
     6961 agatacgctg tcccatctgt cctaggcctt ccggaccgtt gacaaggttt caagccgcac
     7021 ggtacaaagt tcgaggtcgg aagaaggggt ctggcctctg ccagacctcg agcaaacgcc
     7081 aacacccagc aaagaatgtt caaactggcg gcattctcac ccgcccaacc ccaggcgcac
     7141 ggagttgcgc ctctacccgg ggcggccgca gcgggttaga acaagcaatc ggtttcaacg
     7201 ttcataaagg aggaatcccg atgagtgacg tagccccgga aatcaaggtg ggcgataccg
     7261 ccccggactt taacctgaaa gaccaggacc agaaggatgt gaagctgagc gactataaag
     7321 gcaagaagaa tgtggtgctc gccttctatc cgctggattg gagccccgtc tgtcaaggcg
     7381 agaacaagtg cctgaccgac gactttccca agttccaatc ggccaacgca gaactcttcg
     7441 gcatcagctg cgacagcttc ttctcacaca aggcctgggc ggattccatg gatctcaagc
     7501 atcgcctctt gtcggatgtg catcggacca cggcgaaggc atacggcctc tactttgagc
     7561 cgctcaactg ctccaagcgc gcgaccgtca tcgtcgataa gaacggcaag gtcgcctatg
     7621 tgaaggtgca ggagatcaag gtggcgcggg aagacaagga aatcctcgca gccctcgcaa
     7681 aattaaacta accagtgctg aggactgggt gctgagatcg gggagaatat ctggtctcag
     7741 cactcagcag tttgggccat gtctggcaca gttcaagacg tcagagacga aaactacaag
     7801 gaattcaccg acagcaccgc ggcggtcgtg gcctacggcc tcgccacctg cgagccctgc
     7861 aagacctacg acccgattct ggaagagacc gccgccaagt ttccacagat caaagtcggc
     7921 aaggcgaaga tgcacgtgcc gggccgctgc cgcgaaatca agaagaccca tacattcgaa
     7981 acctacccga ctacccattt ctttgccaac ggcaagctgc tcctcacccg cgaaggcgtg
     8041 gtcgagccgg ccgaactggc cgccttgatt tcggatcacc tgctcaagta atcgcctgcc
     8101 agtcagggtt acagcggttc gtgcgcaggc ttgtgcacct cccgcaatac cctgccatca
     8161 cagagaaaat ctatcgatgc cgagcccttg agagttactc aggaggcgag ggaacgagag
     8221 accacgcgct tattcaaaag tttcaattca gaagcaggaa gctatttggc cggtcgtggc
     8281 tttgagcagt gctcgtgctg aataagccag gtggaacctt ctttgcggag gatactcgtg
     8341 caaaggccgc ggtcttcggt ctgaatttcg ccgttcgcaa tgagcttgaa tacgtagtcg
     8401 taggtcgccc aggccaaggc tccggacact cgaaccttaa tcccgaagac ctcacccaga
     8461 aaacgagtgg gattcccttt gtcaagttca gcgccgtact cagacaacca cttttctatc
     8521 gtctctctgg tttccgattc tccatcctga attccgacat agtctttcgc gtaggccttc
     8581 agcaccgatt gcgggtctct tgtgcgggaa aattcgctgg tagcttttgc ggaatcgatg
     8641 agcaaggctt gcacggcatc ttcgtcctgg ccggccagga ccaccgatcc ggcaaagact
     8701 tgcagcgcga tgatcattgc cgaaatagcc ggacgtgtca tcggtcatgc tcctctcgca
     8761 tccacaacaa tcgctgccgt ttgccttaac cggagtactc gaaggaaaga tcgcgggtct
     8821 gaacgagccg aaccagctga agggcacccc cgttgacgga tccggccagc cggccccaca
     8881 cgtgcctggc caaggcgttc cccgtcaccg gttggccgag cacccgctca agtacctgcc
     8941 ggtcgaaggc cttcacgact cgctcttgcg cgagccggtc caacgcaccg atatccgtga
     9001 ccatgccggt aacggaatcg atcggaccat ggacggtgac aaaacaatcc caaacatgtc
     9061 cctgattacc ttcctgcacc gccgtaaacg aataacgccg cgtcacactg gcaacgtcca
     9121 acccaccggc agcggtaatg tcggcataca gatcctcgtc ttcatagagg cgaacggtat
     9181 ggagggtgcc gatgtcttgt tgcggttcaa gcttggtcca gagcaggcgg gcgatatttt
     9241 ccgacgtcgg gatgcgatcg ttgaagtacg gcatatccag attgaagttc ttgtggtcga
     9301 actcttcgat aatcgtgagc agcaccctct tcagatcgaa caaattgatc accatgccgg
     9361 ttttgggatc gacctcaccg gacacggtca cttcgagcat gtaattgtgg ccatgcgcag
     9421 gcgggttata gcagggcccg aagactgcgc ggttcttcgc ctcatcccat tccggcttga
     9481 tgtaccggtg cgcggcagcg aactcaatgc gttttgtcag caatacaggg ggcatcagcc
     9541 tcttctcgct cctacactca acactccatt ctcaggaccc aagactattg taagtcagcc
     9601 agccattctt cgcgcatact ccccggcaca ggcacttctt catgatagct gccatggttc
     9661 acgc
//
LOCUS       DATIFR010000073        14595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_45113, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000073 DATIFR010000000
VERSION     DATIFR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14595
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45113"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1766
                     /locus_tag="VLD60_04025"
     CDS             138..1766
                     /locus_tag="VLD60_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44153.1"
                     /translation="MKIWDSLPKNQYLSLAPWCWVQLESAEAPGPFPFVAGVAPEVVA
                     SLQEAHTLLASSIDTAISDVFSKRAPLDDPDRQRRLEDAYAELVNSRPYLRQYIKCQR
                     NQDGTFQWEFPTDPTKSSTVTNGGLRIFHSVKRQAIPIGFDQRPLGPMVGKLLGMLDG
                     THQTEEIRTVVMSAPREAQALLTRLMESLHQYECLRSSSQAPIRQRWFDTVQDRDTVH
                     LGHAALLYRQQQNIFLFDPWLLPWFAESSVPSLWGSLLPKPTAVFLTHDHDDHVDPRT
                     LLHLPKETSIIIPSRRNRKKLFYDYLSLLRELGFGRVVELAHGETWPFEGGTIVSVPF
                     YGEDPCDLEMPRNCYLISDRGHNVLVHADSGPTNIGRSALKEHIIERLVDTYGPIPLV
                     FASQQQLLEVRSHAAHGPLSHPGKWLDVGENGYLTNAYLADLCVTAKAKLFVSYATGG
                     ADWYPDHLSFMFSQRNPARTALLTAHWEPPEKLKDLLAPHGCRYHYAHALERYRATND
                     GPIDVQSTDGALDPLSLYRLAHGEPPFMKAGSGA"
     gene            1799..2821
                     /locus_tag="VLD60_04030"
     CDS             1799..2821
                     /locus_tag="VLD60_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HSB44154.1"
                     /translation="MTVSMPLTLVTGVAGFIGFHVVRRLIARGDHVIGLDNVNDYYDV
                     RLKAARLAELTPLEKFQFVKLDLANRQGMKELFTEYPISRVVHLAAQAGVRYSLVNPY
                     AYTDSNIEGFMNILEGCRQAKVEHLVYASSSSVYGGNTKMPFSIHDNVDHPVSLYAAS
                     KKANELMAHCYAHLYRIPCTGLRFFTVYGPWGRPDMALFIFTKAILEGKPIEVFNHGK
                     MRRDFTYIDDIVEGIVRTLDHTAQPNPAWAADRPDPGTSSAPARIYNIGNHQPVELLR
                     FIEVLEQALGKKAEKKLMPLQPGDVPATYADIDDLARDVGFAPATPIEEGIPRFVKWY
                     REFYKI"
     gene            complement(2878..3459)
                     /locus_tag="VLD60_04035"
     CDS             complement(2878..3459)
                     /locus_tag="VLD60_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44155.1"
                     /translation="MRPHAMFIMVAATALAMFSTPAFSETATTAEFDPARMWECSQAD
                     GSSIYTNKERPGCKLMTLKPLSIVPSLDNMPTYRPAVAAAPHYDIPGYVERQQAGMSG
                     GGQNIPDWAKDWHASVASSDSVQAQVCSMYGEWLNLSQKTRGGFYFGSDPSYGGDLSG
                     RNQRGASYSFYDNARYVTLSKIFGQGFVPIGCQ"
     gene            complement(3597..4325)
                     /locus_tag="VLD60_04040"
     CDS             complement(3597..4325)
                     /locus_tag="VLD60_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSB44156.1"
                     /translation="MIRAIIFDFNGVIADDETPHVVCFQQALAEVGLSLTKDEYYGTY
                     LGMDERTCARMLLVARDGICEEGILSRIAQRKAELFRACTAIHKPALFPGIVEFVKAA
                     KPAYRLAIASGGRREQIDAALAGTPIEQDFELIVSAEDCPIGKPDPAIYQYTCERLNA
                     TGIKSHAIAPAHCLVIEDSLAGIRSAKAAGMRVFAVATTYPVDKLDGADGVLTSLHRI
                     TPDLAIGKIVQTGQESRLSITQPR"
     gene            4524..6524
                     /locus_tag="VLD60_04045"
     CDS             4524..6524
                     /locus_tag="VLD60_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB44157.1"
                     /translation="MTDYNVLGNLLVIFSVSIAVVFAFHQFRLPSIAGFLVAGALIGP
                     NGLNLISDIGTVQVLAEIGVVLLLFTIGIEFSLVQLASLRRLLFVAAPIQVGAVLLVV
                     WLGATLVGLPWQECIFWGFLLSLSSTAIVLKALAERGDSDSIHGRTTIGILVFQDLAV
                     VPMMLLTPTLASRTEGSAVSILVTLGTSILVVGLVIAAAWYIVPKLLEHIVRSRSREL
                     FLLTIIVLCLGIAWLTSLGGLSLALGAFIAGLVISESEFSHQAMAEVLPFRDSFNSLF
                     FVSIGILMDWRVLFSHPLLVPGLLLAVILVKFLTGAGASLALEIPPRAAIMVGIALAQ
                     VGEFSFLLAQQGQEYGLLRGDPYQVFLAVSILSMIVTPFLMQWSPSMARRVEAVQRLR
                     HWLPSRTEAHALQTEGKNIRLKDHVIIVGYGLNGRNLARVLSETEIPHLALDLDGDTV
                     RRESRHGVPIYFGDATNPNVLRHAKIEDARVLVVAISDPFITRRTVQLAKGLNPKIRV
                     VVRTRYLRELEELHELGADDVVPEEFETSIEIFALVLRTYNMPHDFVINKAEQVRREG
                     YALLRRSELPELAHHLRGGTLTDAEVETCRIDDDSPAVGKTFADLSIRPRTGASVIAW
                     TRGGVTESNPSLKTRLLVGDIVVLLGSRPQIRRAISMLLSGD"
     gene            complement(6575..7663)
                     /locus_tag="VLD60_04050"
     CDS             complement(6575..7663)
                     /locus_tag="VLD60_04050"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HSB44158.1"
                     /translation="MNSDLITEIKPSKSGPLLGFWYPACQSSDLRPGTMKGVVLLGLP
                     ILACKTSRGVVAAMRDICPHRGMPLSYGQMKDDCVECAYHGWRFDSTGRCRGIPALVE
                     DSPIQADKIGITTYPCRERDGYVWVFLADPQQPQQAVPDVPPLSLPSSPYRMIQISTL
                     LDCTIDDGIVGLMDPAHGPFVHQSSWWRTQASMHEKAKTFEPIPNGFRMVPHAPSKNS
                     GPYKILNLFYGGPLTTTIDFVLPNQRFEFVQCGNMFVSTRATVTPITDHQCRIDFCAA
                     WNALGWLPFSKTLFRYFANIFMKQDKEAMERQAVGLRYRPSLMLIDDADTPAKWYYKL
                     KAAHLASVQAGQPLDHPLKGRVTLRWKS"
     gene            7899..8432
                     /locus_tag="VLD60_04055"
     CDS             7899..8432
                     /locus_tag="VLD60_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSB44159.1"
                     /translation="MMTTTQMDPAVALADLQATRPDKVTIVLLSGDLDRAMAAFIIAT
                     GAAAMGMQVTMFFTFWGLNTIRRKGATSSAKDWLRRMFGLLNKGGADSLPLSRFHFGG
                     LGTKMMQKVMKQNRMPGVPELMQTALDLGVRFIACTTTMGLMGISKDTLIEGIDQFAG
                     VTTYLAEAKQGNVNLFI"
     gene            8522..8767
                     /locus_tag="VLD60_04060"
     CDS             8522..8767
                     /locus_tag="VLD60_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSB44160.1"
                     /translation="MIQADVKLDTLGYFCPMPIIMTSKKIKELTLGQVLEVVSDDEGI
                     KKDMPAWCDTTGHQMVGLEEEQAKSGPIYKAFVKKTK"
     gene            8859..10532
                     /gene="ftcD"
                     /locus_tag="VLD60_04065"
     CDS             8859..10532
                     /gene="ftcD"
                     /locus_tag="VLD60_04065"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HSB44161.1"
                     /translation="MEPLVECVPNFSEGRNRTTVQALIDAVASVTGVRVLDHTMDRDH
                     HRCVLTFVGEPEGMIEAAFRAILVATDLIDLRQHSGAHPRVGAADVVPFVPIRGATMQ
                     DCVQLAKRLGQRVGQELDIPVFLYERAASHPDHAALESVRRGGLEGLAYRLASDPDWS
                     PDFGPAHLHKTAGAIVIGARQPLIAFNVNLHSKDLTVAQSIAKAIRQSNGGIPHLKAI
                     GVELASRRMVQVAMNLTDYQETPIHVAFEAVQAQAALHGVEIAGSEIIGLVPQSALVE
                     AAVDALRLDHFDPAQVLETRIETALSSEGADHMDQAEFGPKDLRRLSVSQLLDAVAAA
                     TPVPAGGAVAALAGALAGSLGLMAARLSQQHATEHRLDEIGRRLSDLLQADGEAYRTF
                     IEATRLPKSDPDRPTALSSALHVATEIPLEIAERSADAGALLHACSGGVKSRVRSDLK
                     VGLFMALAAGEAGVHTAKENLKLQPNQRLRRALQPRILRVSQYLEDLKCLCYTPPLSQ
                     RGAGKKLAQASPGKVRKRNEWKSKSSITTSKKRSKLRRQSSRGKGSSGN"
     gene            10442..10636
                     /gene="rpsU"
                     /locus_tag="VLD60_04070"
     CDS             10442..10636
                     /gene="rpsU"
                     /locus_tag="VLD60_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HSB44162.1"
                     /translation="MEIKVFNNNVEKALKVAKTKLAGEGLFRELKRRRYYEKPSVRKK
                     AKQREAQRRRQKWLSKRKPE"
     gene            10706..11365
                     /gene="nth"
                     /locus_tag="VLD60_04075"
     CDS             10706..11365
                     /gene="nth"
                     /locus_tag="VLD60_04075"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSB44163.1"
                     /translation="MRQDQIHAAIRIVKREVRQWQEPVVGVVARESNRDPFRILISCL
                     LSLRTKDKTTSEASARLFALAQTPSALLKLPLKKIEKVIYPVGFYRTKAKSIHAICRR
                     LLDVYGGKVPDSIDELVTLSGVGRKTANLVVTVGYRKPGICVDIHVHRISNRWGYVKS
                     KTPEETEAALRRKLPKQYWITFNDLLVPYGQNLCQPVSPYCSQCKLTEFCDRVGVKRS
                     R"
     gene            complement(11449..11871)
                     /locus_tag="VLD60_04080"
     CDS             complement(11449..11871)
                     /locus_tag="VLD60_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44164.1"
                     /translation="MSQPKPKFVLFAHNATYDKLHQVATLGLTAAAMGKDVIVILLFW
                     TIKKLAEGKFDQIDFPPEYAASADEVARLLKEKKVPKISEMFEEAKLVGTFRVIACSA
                     GLEYMGVDSEVVAKRVDEVMGLPAILSLTAHAETKLFI"
     gene            complement(11874..12221)
                     /locus_tag="VLD60_04085"
     CDS             complement(11874..12221)
                     /locus_tag="VLD60_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44165.1"
                     /translation="MRATSCRVFLPLPLLLWLAATGADPGCALAAADELRIEITKKGL
                     GKEVIITQGDREWFMMVEVTPENTVIVRQEKDQDKYLVDESETHDRPLAPIEVDAAIN
                     DYVNSVKARAPKK"
     gene            12320..12751
                     /locus_tag="VLD60_04090"
     CDS             12320..12751
                     /locus_tag="VLD60_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSB44166.1"
                     /translation="MEWLLSTVQQYVSTDTLVTLTVLSIVFFIGSLIAIPYILVRLPA
                     DYFDIRIPRPWMKDHHPILRVMGHIVKNAVGAIFLFVGFLMLFLPGQGVLTMVIGVSM
                     LDFPGKRKVEASLIGQPTVLGVINSMREKFGKPPLILAPDP"
     gene            12840..>14595
                     /locus_tag="VLD60_04095"
     CDS             12840..>14595
                     /locus_tag="VLD60_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HSB44167.1"
                     /translation="MSDSSHPKTLYLIDGSAYIYRAFFALPALNNSKGLQTNAVYGFT
                     TTLMKIIREHQPDCLAVAFDEKGPTLRHEEFKEYKAQRPPMPDGMKAQIPYIHRVVEA
                     MNIPAVRQAGYEADDLIGTLARQAERAGYDVVIVTGDKDMLQLLTPHIRIYDPVKNKW
                     SGEAECRERFGVEPARVIEVMGLMGDSTDNIPGVKGIGEKTAMKLITQFGTIDELLQR
                     VDEVTPARIKTLLIEQADNARLSRRLATIQTESPVEFHPESYRIKPPHSDQLADLLRE
                     LEFTTLLKIVQPAPRTAEAKSDQATAIIDNEASAQRFVSELPKGGFLGVCCLLAGSTG
                     VDTDVRGIALSTGEKTAFVPIDVRTFMRPITALLHDQARTKVVHDLKATLLAFHRIGL
                     TLAAPYLDTMIADYLLNPNRRDHQLETIALEVLGDRLGSGKQGRATSQSLFEVETGSQ
                     EEAAEAAAVLARLAPALTQRLAEQGSLNLFTDVEMPLVPVLAEIERNGFLLDVEALHG
                     LSKELERDLDRMMETIAGLAGGEFNINSPKQLATVLFDKLGLKPVRKTKTGYSTDEDT
                     LTQLSTQHELPAQILNYRS"
BASE COUNT         3025 a         4116 c         4348 g         3106 t
ORIGIN      
        1 tatgagccga cgatcagcaa actggaattc acacgtaagg ggtaaggggt gaggggtgag
       61 gtgatgttcg gagtgcgctt cctcaaaccg agagtttttc tcgccttacg cctcacgcct
      121 cacgtttcac gcatattatg aagatctggg acagtcttcc gaagaaccag tatctcagcc
      181 tggcgccctg gtgctgggtg caactcgaga gtgcggaagc gccgggcccg ttccccttcg
      241 tcgccggtgt ggctccggaa gtcgttgcga gtcttcagga ggcccacacc ttgctcgcca
      301 gctccatcga cacggccatc agcgacgtgt tttcgaaacg tgccccgctc gacgaccccg
      361 atcgtcagcg tcggttggaa gacgcctatg ctgagttggt gaattcccgg ccttatctgc
      421 gccagtacat caagtgccaa cgcaaccagg acggcacgtt tcaatgggag ttcccgaccg
      481 atcccaccaa gtcgtcgacc gtcacgaacg gcgggcttcg gatcttccat tcggtgaaac
      541 ggcaggcgat cccgatcgga ttcgatcagc gcccgctcgg gccgatggtt ggaaaactgc
      601 ttgggatgtt ggatggcacc caccagaccg aagaaatcag aacggtggtg atgtcggctc
      661 cacgtgaagc gcaagctctg ctgacacgac tcatggaatc gctgcatcag tacgaatgtc
      721 tgcgcagttc gtcgcaagcc ccgatccgtc aacggtggtt cgacacggta caggaccgcg
      781 ataccgtcca cctgggtcat gcggcgttgc tctaccggca gcaacagaat atctttctgt
      841 tcgatccctg gctcttgccc tggttcgctg aatcttccgt gccttcgtta tggggatcgt
      901 tgctgccgaa accgacggcg gtctttttga cgcacgacca tgatgaccac gtcgacccgc
      961 gcacgctgct ccatcttccc aaggagacgt cgatcatcat tccgagccgc cgtaaccgca
     1021 agaagctctt ctatgattat ctctcgcttc tgcgggagct tggattcgga cgtgtcgtcg
     1081 aattggcgca tggggaaacc tggccattcg aagggggaac aatcgtctcg gtcccgttct
     1141 acggagaaga tccctgcgat ctagaaatgc cgagaaactg ctatctgatc agcgatcgag
     1201 gccacaacgt cctggttcac gcagacagcg gacctacgaa catcgggcgg tcggcgctca
     1261 aggagcacat cattgagcgg ctcgtcgaca cctatgggcc gatcccgctg gtctttgcct
     1321 cgcagcagca attgctggag gttcgcagtc acgcggccca tggtccgctc tcgcatcccg
     1381 gaaaatggtt ggatgtcggc gagaacggct atctcacgaa cgcgtatctg gccgaccttt
     1441 gcgtgacggc caaagccaag ctgttcgtgt cctatgccac cggcggggcc gattggtatc
     1501 cggaccatct ctcgttcatg ttcagccaac ggaatcccgc aaggacggca ttgctgaccg
     1561 ctcattggga accgccggaa aagctgaaag accttcttgc acctcacggt tgtcggtatc
     1621 attatgctca tgcgctcgag cggtaccgtg cgacgaacga tggccctatt gacgtgcaat
     1681 cgaccgatgg cgcactcgat ccgttgagcc tctatcgtct ggcccatggc gagccgccct
     1741 ttatgaaggc gggtagcggg gcctgaccta accgctgtcg gaatcacgag gaatctggat
     1801 gactgtatcg atgcctttga cgctggttac cggggtagcg gggtttatcg gttttcatgt
     1861 cgtccgacga ctcatcgcgc gcggcgacca cgtcatcggg ctcgataacg tgaatgacta
     1921 ttacgatgtc cgcctcaaag cagcacgcct tgcggaactg accccgttgg agaagttcca
     1981 gttcgtgaaa ctcgacctgg ccaatcgcca gggaatgaaa gaactcttca ccgaatatcc
     2041 gatcagccgc gtcgttcatc ttgctgcgca ggccggcgtg cgctattcgc tcgtcaatcc
     2101 ttatgcctac accgacagca atattgaagg ctttatgaac attctggaag ggtgccggca
     2161 ggcgaaggtc gagcacctgg tctatgcctc gtccagttct gtgtatggtg ggaacacgaa
     2221 aatgcccttc tccatccacg acaacgtgga ccaccccgtc tcactgtacg ctgcgagcaa
     2281 gaaggccaac gagttaatgg ctcattgcta tgcgcatctg taccggatcc cgtgtaccgg
     2341 gctgcggttt tttacggtgt acgggccttg gggccggccg gacatggccc tcttcatttt
     2401 caccaaggcg attctggagg ggaaaccgat cgaggtgttc aaccacggga agatgcggcg
     2461 ggactttacc tatatcgatg acatcgtgga aggaatcgtc cggacgctgg accatacggc
     2521 ccaaccgaat ccggcctggg cggccgacag gccggatccc ggcaccagtt cagcgccggc
     2581 caggatttac aacatcggga atcaccagcc ggtggaactg ttgcgtttca tcgaggtgtt
     2641 ggagcaggcg ttgggaaaga aagcggagaa gaaactgatg ccgttgcagc cgggcgatgt
     2701 gccggcgacc tatgcggata tcgacgacct cgctcgcgac gttggatttg caccggcgac
     2761 tccgattgag gaaggcatcc cccgtttcgt gaagtggtat cgagagttct acaaaatata
     2821 agagggcgtc aggctcctgt gcgcccgacg ccccctatgc tctgctcgcg tgacttctta
     2881 ctgacagccg atcggcacga aaccttggcc gaaaattttg gacaaggtca cgtagcgcgc
     2941 gttgtcgtag aacgaatagc tggcaccccg ctggttccgt cccgagagat ccccgccata
     3001 ggatgggtca gatccgaagt agaacccgcc gcgcgttttc tggctgagat tgagccattc
     3061 gccgtacatt gagcacacct gagcctgcac ggaatctgac gaggccacgc tcgcgtgcca
     3121 gtccttggcc cagtccggaa tattctgccc tccgccgctc ataccggcct gctgccgttc
     3181 gacatatccg gggatatcat agtgaggggc cgcggccact gcgggacgat aggtcggcat
     3241 gttgtcgagc gacggcacga tggacagcgg cttgagcgtc atcaatttac agccgggccg
     3301 ttccttattc gtgtagatcg acgagccgtc cgcctgtgaa cattcccaca tccttgcggg
     3361 atcgaactcg gccgtggttg ccgtttccga aaacgccggc gtcgagaaca tggccagcgc
     3421 ggttgctgcg accatgataa acatggcgtg tggccgcata aggcacctcc cctgaatctg
     3481 tggcaaagac aacgtgcgca tggtgaatcg cctttcttga tagtacttaa tgatcgtgtt
     3541 gacaagctat gacagcagcg aggagggcgt ctattcgtct ttggaggggt atgttgctac
     3601 cgaggctggg tgatcgagag acggctttct tggccggttt gaacaatttt gccgatcgcg
     3661 aggtcaggcg taatcctgtg cagactcgtg agcacgccgt cggccccgtc tagtttatca
     3721 acgggatagg tcgtcgccac ggcaaagacg cgcatgccgg ctgccttggc cgagcggata
     3781 ccggcaaggg aatcttcaat cacgaggcag tgagccgggg cgatcgcgtg cgactttatg
     3841 ccagtagcat tgaggcgttc gcatgtatac tgataaatcg caggatcggg ttttccgatt
     3901 ggacagtctt cggctgagac gatcaactca aaatcctgtt cgatgggagt tcccgcaaga
     3961 gcggcatcga tttgctcccg gcggccgccg gacgcgatgg ccagcctata ggctggcttg
     4021 gcggctttca cgaactcgac tatgcccggg aagagtgccg gcttgtggat cgcggtgcag
     4081 gcgcgaaaca gttctgcctt ccgttgtgcg atacgcgaca atattccttc ctcgcatatt
     4141 ccgtcgcgtg cgacaagcag catgcgcgcg caggtccgtt catccatgcc gaggtaggtg
     4201 ccgtaatact catccttcgt cagagaaagc ccgacctcag ccagcgcctg ctggaaacag
     4261 acgacatgcg gagtctcgtc gtcggcaatg acgccgttga agtcaaaaat gatggctcgg
     4321 atcatggtgg ccccttctcc gtctgcttct ccactctact caaccgtcta ttcaacgaca
     4381 agcaccttta ccttgtccct ttgctcttgc tggggtattc tccgccctgc gctcgtgaaa
     4441 cgtgatgggt gaaaggtcag ctgctcgaat tgttccgcgt gcatctcctt acccctcacg
     4501 cctgactcct tacggtattt cgtatgactg actataacgt cctcggcaat cttctcgtca
     4561 tcttttcggt atcgatcgcc gtcgtcttcg cgtttcatca gttccggctc ccgtcaatcg
     4621 ccggattcct ggtggcaggc gcattgatcg gtccaaacgg actgaatctc atctccgata
     4681 tcggcacggt ccaagtgttg gccgaaatcg gcgtagtcct cctgctgttt acgatcggaa
     4741 tcgagttttc gttggtgcaa cttgcctcgt tgcgccgcct gctcttcgtg gcggcgccga
     4801 tccaggtagg cgccgtactc ctcgtcgttt ggttgggagc gacgctggtc gggcttccct
     4861 ggcaggagtg cattttctgg ggatttctgc tctcgctcag cagcaccgcg atcgtactga
     4921 aggcgttagc cgaacggggc gatagcgatt cgatccacgg ccgcacgacc atcgggattc
     4981 ttgtcttcca ggatctggcc gtcgtgccga tgatgctcct gacgccgacc ttggccagtc
     5041 gaacagaagg atcggccgtt tcgatcctcg tgaccttggg gacgtcgatt ctggtcgtgg
     5101 ggctggtgat tgccgccgcg tggtatatcg tgcccaagct cctcgagcac attgtccgca
     5161 gcaggagccg cgaactattt ctgctcacaa tcatcgtgct gtgtctcggc atcgcgtggc
     5221 tgacgtcgtt aggcggcctg tcgctggccc tgggagcctt cattgccggc ctggtcatat
     5281 ccgaatcgga gttcagccac caggcgatgg cggaggtgct gccctttcgg gacagtttca
     5341 acagcctgtt ttttgtgtcg atcggcattt tgatggactg gcgcgtcctg ttcagccacc
     5401 cgctgctggt gccgggatta ctgctggcgg tcatcctggt caagtttttg accggtgccg
     5461 gcgcgtcact ggcactggag attcccccgc gtgcggccat tatggtgggg atcgccctgg
     5521 cccaagtcgg tgagttcagt ttcctgttgg cccagcaggg gcaggagtac gggttgttac
     5581 gcggcgatcc ctaccaagtg tttcttgcgg tctcgatcct ctcgatgatt gtcacgccgt
     5641 ttttgatgca gtggtccccg tcgatggcgc gccgggtcga ggcggtgcag cggctgcggc
     5701 attggcttcc cagccggacg gaggcccatg cgctgcagac cgagggaaag aacatccggc
     5761 tgaaggacca tgtcatcatt gtcggctacg gattgaacgg aagaaatctc gcccgggtgt
     5821 tgagcgaaac cgagatccct cacctggccc tggatctcga cggcgacacc gtccgccgcg
     5881 aatcccggca cggcgtaccc atctacttcg gggatgcgac gaatccgaac gtcttgcgcc
     5941 atgccaagat cgaggatgcc agagtgttgg tcgtggcgat ctccgatccg tttatcacga
     6001 ggcggacggt gcagttggcc aaagggctga acccgaaaat ccgagtcgtg gtgaggaccc
     6061 gttacctgcg tgagctggag gaattgcacg aactcggagc ggacgacgtg gttccggagg
     6121 aatttgaaac gtcgatcgaa attttcgcgc tggttctccg gacctataat atgccgcatg
     6181 actttgtcat caataaggct gagcaggttc gacgggaggg gtatgcgttg ctgcggcgca
     6241 gcgagttgcc ggagctggcg caccatctgc gcggcggaac gttgaccgat gctgaagtcg
     6301 aaacctgccg gatcgacgac gactcaccgg ccgttgggaa aacgtttgca gacctctcga
     6361 tacggccacg aacgggggct tcggtcattg cctggacgag aggtggagtg acggaatcga
     6421 atccctcgct gaagacgcgg ctgctggtgg gggatatcgt ggtgttattg ggatcgcgac
     6481 cgcaaattcg gcgggcgata agcatgctgc tgtcgggtga ctagcgcgat ccttctccct
     6541 ccgctctcgg gcaggtgctt tcagcgaaat cgtcctagct cttccaacgg agcgtcaccc
     6601 ggcctttcaa cggatggtcg aggggctgtc cggcctgcac cgaagccaag tgcgcggctt
     6661 taagtttgta ataccacttg gccggcgtgt ccgcatcgtc gatcaacatc aaggacggtc
     6721 tgtagcgcag tcccaccgcc tggcgctcca tcgcctcctt atcctgcttc ataaatatat
     6781 tggcaaagta gcggaagagg gttttggaga atgggagcca acccaaggcg ttccatgcgg
     6841 cgcagaaatc gatccgacat tgatggtcgg taatgggcgt caccgtggcc ctggtggaga
     6901 cgaacatgtt cccgcactga acgaactcga atcgttggtt gggcaatacg aagtcgatgg
     6961 tggtggtcag aggcccgcca tagaacaagt tcaagatctt atacggcccg ctgttcttgg
     7021 aaggggcatg ggggaccata cggaaaccgt ttggaatcgg ttcaaaggtc ttggccttct
     7081 cgtgcatgct cgcttgcgta cgccaccagg aactctgatg aacaaagggg ccgtgagccg
     7141 ggtccatcaa gcccacaatc ccatcgtcaa tcgtgcaatc cagcagggtc gatatctgga
     7201 tcatgcggta gggagaggaa ggaagcgaga gcggcggtac gtccggaacc gcttgttgcg
     7261 gctgttgggg atcggcgagg aacacccaca cgtacccgtc ccgctcgcgg cacggataag
     7321 tcgtgatgcc gatcttgtcc gcttgaatcg gagagtcttc caccagtgcc gggatccccc
     7381 ggcagcggcc ggtgctatcg aagcgccagc cgtggtaggc acactcgaca cagtcgtctt
     7441 tcatttggcc atacgagagc ggcataccgc ggtgaggaca gatatcacgc atcgccgcga
     7501 ccactccccg cgatgttttg caagcgagga tgggcaggcc caataatacg acgcccttca
     7561 tcgtccccgg tctgagatcg gaactctggc aggccgggta ccaaaatccg agtaacggac
     7621 cgctcttaga gggtttaatt tcggtaatca ggtctgaatt cattgacggg gaagggagta
     7681 cgtgagagga ccagcctaga ctgcagaagc agcttgatgg tgaatataac ggctgggtta
     7741 tcgaacgtca agccgggaga gtctggtttg gctgtctccc ttcttgcagc aaggagcctt
     7801 ccgcttcccc tcttccttga catgaaaaag aagcggggac tatagtgaaa aaatggcgta
     7861 aacagcgggt ttcttgcgcc accgacccaa gagagggcat gatgacaacc acacaaatgg
     7921 atccagccgt ggccctggcc gatctccagg cgacgagacc ggacaaggtc acgatagttt
     7981 tgctgagcgg ggatttggat cgagccatgg cagcattcat cattgccacc ggcgcggcgg
     8041 cgatgggtat gcaagtcacc atgtttttca cgttttgggg attgaacacc atccgtcgga
     8101 aaggcgcgac gagttcggcg aaagattggc tccgccgcat gtttggcctc ttgaacaagg
     8161 gaggtgccga cagcctaccg ctctcccgat ttcacttcgg cggactcggc accaagatga
     8221 tgcagaaggt catgaagcag aatcgaatgc ccggcgtgcc ggaactgatg cagactgctt
     8281 tggatctggg cgtgcggttc atcgcctgta cgaccaccat gggactcatg ggtatctcca
     8341 aggacacgtt gattgaggga atcgatcaat tcgccggtgt gaccacatat ctggcggaag
     8401 ccaagcaagg caacgtcaat ctctttatct agactcgagt gctgagtgct gtgtgttgag
     8461 cgcagaggga atactctcga gtctcagcat tcatcgctca gtactgacaa ggaaggacgg
     8521 tatgatacaa gccgatgtga agctcgatac actcgggtac ttctgcccga tgccgatcat
     8581 catgacgtcg aagaaaatca aagaactgac gttgggccaa gtgcttgaag tcgtgtcgga
     8641 cgacgaaggc atcaagaagg acatgccggc ctggtgtgat acgaccgggc atcagatggt
     8701 ggggctcgaa gaggagcagg ccaagtcggg accgatctac aaggcatttg tgaaaaagac
     8761 gaagtagagc ctacggattt acagtgcaaa gacagtcgga agccggaggg atgatcgatc
     8821 agacctccgg cttcgtattt tgaatgagca gagggttcat ggagcctctc gtcgagtgcg
     8881 tgccaaattt cagtgaaggc cggaatcgga cgacggttca ggccctgatc gacgcggtgg
     8941 cgtcggtcac gggagtccgg gtgctggatc acaccatgga ccgagatcac caccggtgcg
     9001 tcctgacctt cgtcggcgaa ccggagggca tgatcgaggc ggcgtttcgc gcgatccttg
     9061 tcgcaacgga tctcattgat ctacgacagc acagcggtgc gcatcctcga gttggggcgg
     9121 cagacgttgt tcctttcgtg ccgatcaggg gtgcgacgat gcaggattgc gtgcagctgg
     9181 ctaaacggct tggacaacgg gtaggacagg agctcgacat accagtcttc ttgtacgaac
     9241 gggcggcctc ccatcccgat catgccgcgc tcgagtcggt tcgtcgagga gggctggaag
     9301 gcctggccta tcgcctagct tccgatccgg attggtctcc cgatttcggt cctgctcatc
     9361 tgcacaagac agcaggtgct atcgtgatcg gggcgcggca accgttaatc gcatttaacg
     9421 ttaaccttca ttcaaaggac ctcaccgtgg ctcagtcgat tgccaaagcc atcaggcagt
     9481 cgaacggggg cattcctcac ctgaaggcga taggggtgga attggccagc cgtcgcatgg
     9541 tccaggtcgc gatgaacctg actgactacc aagaaacgcc catccacgtc gcatttgaag
     9601 cggtccaggc tcaagctgcc ttacatggtg tagagatcgc cgggagcgag atcatcgggc
     9661 ttgtgccgca gtcggcgctt gttgaagcag cagtcgatgc attacgtctc gaccatttcg
     9721 atcccgcgca ggtgctggaa actcgaattg agacagctct ctcctcagaa ggcgccgatc
     9781 acatggacca ggcagagttc ggcccaaagg acctgcgacg gctatcggtt tcacaactct
     9841 tggacgctgt cgccgcggcc acaccggtcc ctgccggagg tgcggtcgcc gcgctcgccg
     9901 gcgcgctcgc cggctcgctc ggcctgatgg ccgcgcgact gagtcagcag catgccacag
     9961 agcaccggtt ggacgagatc ggccggcggt tgagcgactt gctgcaagcc gacggcgagg
    10021 cctatcgaac ttttattgag gcgacgaggt tgccgaagtc ggacccggat cgccctaccg
    10081 cactttcttc tgcgttacat gtggcgacgg aaatccctct ggaaattgcc gagcggtcgg
    10141 cggatgccgg agctctgctc catgcctgct caggaggggt gaaatcgcgg gtgcgatccg
    10201 atttgaaggt cggcctgttt atggcgctgg cggcaggtga ggcaggggtg cacacggcga
    10261 aagaaaatct gaaactacag ccaaatcagc ggcttaggcg tgcattgcaa ccaagaatcc
    10321 ttcgagtttc gcaatatctt gaggatctga agtgcctatg ctacactccg ccccttagtc
    10381 aacggggtgc tggaaaaaaa ttagcgcagg catcgcctgg gaaagtgcgg aagcggaacg
    10441 aatggaaatc aaagtcttca ataacaacgt cgaaaaagcg ctcaaagttg cgaagacaaa
    10501 gctcgcgggg gaagggctct tccgggaact gaagcggcgg cggtactacg agaagccgag
    10561 cgtcaggaag aaagccaagc aacgtgaggc tcagcgtcgc cgtcagaagt ggttgtcgaa
    10621 gcgcaagccg gagtagagag tttccttcgg gcctgttcct tcgaattagc atctctttac
    10681 agctatcctg ctcggtttct catccatgcg ccaggatcag atccatgcgg cgatcaggat
    10741 cgtcaagcgc gaggttcgcc aatggcagga accggtcgtg ggggtggtgg cccgcgaatc
    10801 taaccgagat cccttccgca ttctgatttc ctgccttctg agcctgagaa ccaaagacaa
    10861 gacaacgagc gaagccagcg cccggttgtt cgcgctcgcg cagacgccat cagcactact
    10921 caagcttccc ctgaagaaga tcgagaaggt catctatccg gtcgggttct atcgaactaa
    10981 agcgaaatca atccacgcca tttgccgccg cctcctggat gtctacggag ggaaagtccc
    11041 agactccatc gatgagctgg tcacactctc gggagtcggg cgtaagacgg cgaatctcgt
    11101 cgtgacggtg ggctaccgga agccgggtat ttgtgtggat attcacgtgc accggatcag
    11161 caatcgctgg ggctatgtaa agtccaagac accggaggag acggaagcag cgttgcgccg
    11221 aaaacttccg aaacaatatt ggattacctt caacgatctg cttgttccgt acgggcagaa
    11281 cctctgccag cctgtctcac cttattgcag ccaatgcaaa ctgacggagt tctgtgatcg
    11341 ggtgggagtg aagcggtcgc ggtaagagcg cgcaggcgag aagcgcggga cgagcgagaa
    11401 acgcaagata cccctcgcgc aagtctcgca tttcgcgcga gtctcgaatt aaatgaacag
    11461 cttggtctcg gcgtgcgcgg tcagcgagag aatggccggc agacccatca cctcatcgac
    11521 tctcttggcc acgacctcgc tatccacacc catgtattcc aagccggcgc tacaggcgat
    11581 gacacgaaag gtgccgacca gcttggcctc ctcgaacatc tctgagattt tcggcacctt
    11641 cttttccttg agcaggcggg ccacttcgtc ggcggaggcg gcatactccg ggggaaaatc
    11701 aatctgatca aacttgcctt cggccagttt cttgatggtc cagaacaaca agatcacgat
    11761 cacgtccttc cccatcgcgg cggcggtcag ccccagtgtg gcaacctgat ggagcttgtc
    11821 gtacgtggcg ttgtgggcaa agagaacgaa cttcggttta ggctggctca tgctcacttc
    11881 ttcggcgcgc gggctttcac gctgttcacg taatcgttga tggcggcatc gacttcgatg
    11941 ggggccaacg gacggtcgtg ggtttcgctt tcatccacga ggtacttatc ctgatctttc
    12001 tcctggcgca cgatgacggt attttccggt gtcacctcga ccatcatgaa ccactcgcga
    12061 tcgccttgtg tgatgatcac ttccttcccc agccctttct tggtgatttc aatccgaagc
    12121 tcgtcagcgg cagccagggc gcaaccggga tcggcccccg ttgcggctag ccagaggagg
    12181 agaggcagtg gcaggaagac acgacacgag gtggcgcgca tggcaggatt atagccttcg
    12241 cgagaggcgc agtaaaccgg ctttcccgcg tcctctctgg cttgagtgcc cctaggtcgc
    12301 atgttaggat gcccgtccga tggagtggct tctctcaact gttcagcagt atgtttcgac
    12361 cgataccctg gtcacgttga cggtcctgtc gatcgtgttt ttcatcggtt cgctgattgc
    12421 tatcccttat attctggtgc gacttcccgc cgactacttt gacatccgca tcccccgtcc
    12481 ctggatgaaa gatcaccatc cgatcctccg ggtgatgggc cacattgtca aaaatgccgt
    12541 cggggcgatc ttcctgttcg tcggttttct gatgttgttt cttcccggac agggtgttct
    12601 cacgatggtc atcggagtgt ccatgctcga ctttccgggc aagcgaaagg tcgaggcctc
    12661 actgatcgga cagccgacgg tattgggcgt catcaacagc atgcgcgaga aattcgggaa
    12721 gccgccgctc atcctcgcac cggaccccta acatgctttc agctgtcagc gatcagccat
    12781 cagtgagagg cgaaaatcct gtgctgactg ccgatggctg aaagctgtag gctgtatcta
    12841 tgtccgattc gtcacatccc aagacgctct atctcattga cgggagcgcc tatatctatc
    12901 gtgcattctt cgcccttccg gctctgaaca attccaaagg ccttcaaacc aacgcggtgt
    12961 acggcttcac caccacgctg atgaaaatca tccgtgaaca ccagccggat tgccttgccg
    13021 tggcgttcga cgaaaagggc ccgaccctgc ggcacgaaga attcaaagaa tacaaggctc
    13081 agcgccctcc catgccggac ggcatgaagg cacagattcc ctatatccac cgtgtcgtgg
    13141 aggccatgaa cattcctgcc gtcaggcagg ccggatacga ggccgacgat ttaatcggta
    13201 ccttggcgcg ccaggccgag cgggccgggt atgacgtggt gatcgtcaca ggcgacaaag
    13261 acatgctcca gctgctcacg ccgcacatcc gtatctacga tccggtgaaa aacaaatggt
    13321 cgggcgaggc ggaatgccgc gagcggttcg gcgtcgagcc ggcgcgtgtc atcgaagtga
    13381 tgggattgat gggggacagc accgacaata tcccgggcgt gaaggggatc ggtgaaaaaa
    13441 cggcgatgaa gctgatcacc cagttcggga cgatcgacga attgctgcag cgggtcgatg
    13501 aggtgacacc cgcgagaatc aagaccttgc tgatcgaaca ggcggacaat gcgcgcctca
    13561 gccggaggct ggccaccatt cagaccgaga gtccggtgga gttccatccc gaatcctatc
    13621 ggatcaagcc cccgcacagc gatcagctcg ccgatctgct gcgcgaactc gaattcacca
    13681 cgctcttgaa aattgtgcag cccgcaccta ggactgccga ggccaagagc gatcaggcga
    13741 cggcgatcat cgacaacgaa gcttcggcgc agcggttcgt gagcgaattg ccgaaaggcg
    13801 gctttcttgg cgtctgctgt ctgcttgccg gttctactgg tgtggatacg gatgttcgcg
    13861 gcatcgcgct atcgacaggc gaaaagaccg cgtttgttcc tatcgatgtc cggacgttca
    13921 tgcgacccat caccgcactc ctgcacgacc aggcaaggac caaggtcgtg cacgatctga
    13981 aggcgacctt gctggccttt catcgaatcg gcctgacgtt ggcggctccc tacctggaca
    14041 cgatgatcgc cgattatttg ctcaatccca accgccgcga tcaccaactc gaaaccattg
    14101 cgctggaagt attgggcgat cggctgggga gtgggaagca gggccgggcg acctcgcaat
    14161 cgttgttcga agtcgagacg ggttcgcaag aggaagcggc cgaagcggcg gctgtcctgg
    14221 ccaggcttgc cccggcacta acccagcggt tggcggaaca ggggagccta aacctcttta
    14281 ccgatgtcga aatgccgctg gtgccggtgc tggctgaaat cgaacgaaac ggcttcttgc
    14341 tggacgtgga ggcattgcac gggctcagca aggagctgga acgcgatctc gaccgtatga
    14401 tggaaacgat cgccggacta gccggcggcg agttcaatat caattcgccc aaacaattgg
    14461 caacggtctt gttcgacaaa ctcggcctca agccggttcg taaaacgaag acgggctatt
    14521 ccacggatga agatacgttg acgcaactgt cgacccagca tgagctgccg gcccagattc
    14581 tgaactatcg gagca
//
LOCUS       DATIFR010000074         4429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_812849, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000074 DATIFR010000000
VERSION     DATIFR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812849"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VLD60_04100"
     CDS             <1..849
                     /locus_tag="VLD60_04100"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HSB44168.1"
                     /translation="WKVNTFLAQLEGDQTFSHENIFGLRISYLPTAWLELGATRLTQF
                     GGEGSGGSFPRTMVDCYTNPPNQTGVANCNEQAMLDFRARVPHVPYLVPFPAGMQLYG
                     ELGSEDKWSKIPIPSRAAYLAGLYIPQLFEGDTMDLRIEFADTDYTRRKTSDHLARVW
                     YNNGSFTNGMRQYGFPLGHSMGTDAIDLFIRTTRYLTDELQLGHNLDYQERDRGQPVH
                     ERKYEAAVDLTWWYSRRTQFGFGYTFQHIYNPGQISSVTPFVETFAPNVTASNHLLWT
                     SVTMEF"
     gene            986..3475
                     /locus_tag="VLD60_04105"
     CDS             986..3475
                     /locus_tag="VLD60_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HSB44169.1"
                     /translation="MNISILPHVAKACIWLIVFLMVMPPPLIAQTLGGPGIPSLGGTG
                     LGARDILPGSPVVTNPTAIQPLAPPQTPCPAVPSRNLQSVAHEGTTLNDFWPMEPSSV
                     LPASAEERMKQERAEHQKGLALTDPLQAGQAGGKGLSSPLETQVETKPRGLGTQLETK
                     LQGLGTQPETKPQGAIQDITTKDFTVEEAFSQFSVLHGVKGRLQQFGYDFFDTQATSF
                     APVLDAPVGPDYVIGPLDSLSIHIWNVPDQSLNRSYIAPVERDGMIVIPQIGAIPIGG
                     LTFSQAERAITSRLSAHLKRFEVHVAMARLRTIKVYVVGEVVRPGAYEISSLATVSNA
                     LYAACGPARSGSLRQVRLVRESQLMAEVDFYQFLLAGDRRQDARLQSGDVIVVPPLGP
                     VAAISGAVKRAGIYELKSGIRLTDLLQLASGLTPAANHQRCQLFRLDPGKGRVILDVD
                     LNAVVDTTGKKRPTSNGSDLVIQDGDYVRVGSLPTQVANVVTLAGAVKNPGPYEYKPG
                     MGIRDLLKPDQLTVDAYLDSAELIRTDPFTYQTKVISFSPKALFDSEKGNDLPLQRMD
                     QVVVGTQMRPPNVVLLEGEVKRPGHFTLETGERLSSVLKRAGGLTNNAFPEGIILVRE
                     SVRKRQQAELDRFLASERQRLTAQSASYAAGAVGVPGGAASGQAAEQQVLALRLQQLE
                     AVAVRLELGRVVVNVRSIEELEGTEDDITMEARDRIVIPQPPKTVSIIGSVKNPSTVI
                     YRTGLNFEDYVQQAGGLTEDANKHEMYVMRANGSTEGAYVKIKEMRPGDTVVIPQKIE
                     AKTPTLALWQSIASIVGSVALVAAAISVIGN"
     gene            3564..>4429
                     /locus_tag="VLD60_04110"
     CDS             3564..>4429
                     /locus_tag="VLD60_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HSB44170.1"
                     /translation="MNQANQLNPAIQNEEEVTLLDYWQVVSKRKKSIIAFCSVMVGAA
                     LVVSLLLPKIYESTATLLPQLESNTGFGLGALLGSSAAGSAAQSLGISLPGAPATPTD
                     IFTAMLKSRIMADEVIRQFNLQERYEKKTMQETRLALENATRIVVSKEKVIKITVEDK
                     DPQLAADIANFYVLNLDRLNQTLSVSKARENRKFIEQRVAETKDALVKVENALKEFQT
                     QNRTVAIEAQSKAMIEAAATIQAQIMAQEVQLQVMGSYLSPDNPDVARVQSSINELRK
                     QLRFMESGKGGK"
BASE COUNT         1008 a         1171 c         1303 g          947 t
ORIGIN      
        1 tggaaggtca atacctttct ggctcagctg gaaggcgatc aaactttttc tcatgaaaat
       61 atatttgggt tgcggatcag ttatctgccg accgcttggc tggagctggg agcgacaaga
      121 ctgacccagt tcggcgggga aggaagtggg gggtcgtttc cgagaacgat ggtcgactgc
      181 tacacgaatc caccgaatca gaccggagtg gcaaattgta atgaacaggc catgctggat
      241 ttccgcgctc gagttcctca cgttccctat ctggtcccct tcccggcggg aatgcaactc
      301 tatggggaac ttgggtcgga agataaatgg agcaaaattc ctattcccag ccgggccgcc
      361 tatctggccg gtctctacat cccccagctc ttcgagggag atacgatgga tctccggatt
      421 gaatttgcgg acaccgacta tactcgccga aaaacgtccg accacctggc tcgggtttgg
      481 tacaacaacg ggagttttac gaacggcatg cggcagtacg gatttccgct cgggcacagt
      541 atgggcaccg atgcgatcga tctctttatc cgaaccactc ggtatctgac ggatgagctc
      601 cagctgggcc acaacctcga ctatcaggag cgtgaccgag gccaaccggt ccatgaacgc
      661 aagtatgaag cggccgtgga tcttacctgg tggtattctc gacggacgca gtttgggttc
      721 ggatacacgt tccaacacat ttacaatcca gggcagatca gctctgtgac cccctttgtc
      781 gaaacgtttg ctccgaatgt gaccgcttcg aaccatttgc tgtggacctc ggtgacgatg
      841 gagttttaac cggccttcat aagagagcct tgttgacgat ccggcattct cattcttgac
      901 gcaatgaccg caatgggcta gggttcgtcg gcgttgcgta tgctgtgatg ccgcgacatc
      961 cggccgagac gataagggga caactatgaa tatttctatt cttccgcatg tggcgaaggc
     1021 ctgcatctgg ctcatcgtct tccttatggt catgccccca ccgttgattg ctcagaccct
     1081 cggaggtcca ggaattccat ctctcggcgg aaccgggtta ggagctagag atattctgcc
     1141 cgggtcacct gttgtgacga acccgaccgc gattcagccg ctggccccgc cccagactcc
     1201 ttgtccggcc gtaccgtcac gcaacctgca gagtgtggct cacgaaggca ccacgctcaa
     1261 cgacttttgg ccgatggagc cctcttcagt tcttccggcc tcggcggaag aacggatgaa
     1321 acaagagcgg gctgaacacc aaaagggact cgccttgacg gatccgctac aggcaggaca
     1381 ggccggggga aaggggttgt ccagtcctct tgagacacaa gttgagacga aacctcgcgg
     1441 ccttggaaca caactggaga caaaacttca gggtcttgga acacaaccgg agacaaaacc
     1501 tcagggagcc attcaggaca tcaccaccaa ggattttacc gttgaagaag ccttttcaca
     1561 gttctccgtc ctccatggcg tgaagggacg gttgcaacag ttcggctatg acttctttga
     1621 cacccaagcc acgagttttg caccggtgct cgatgctccg gtcggacccg attatgtcat
     1681 cgggccgctc gactcccttt cgattcatat ctggaatgtg ccggaccaaa gtttgaaccg
     1741 gagttatatc gcgccagtgg agcgtgacgg catgattgtc attccacaga tcggggcgat
     1801 tccgatcggt ggcctgactt tttcccaggc tgagcgagcg attacgagtc ggctgagcgc
     1861 gcatttgaaa cgattcgagg tgcatgtggc gatggcacgg ttgcgtacca tcaaagtgta
     1921 tgtggtcggt gaggtggtca ggcccggtgc gtacgaaatc agctcgctgg cgaccgtgtc
     1981 gaatgctctg tacgcggcct gtggtcctgc acggtccgga tcgttacgcc aggttcgact
     2041 ggttcgcgag agccaactca tggccgaagt ggatttctac caattcttat tagccggaga
     2101 tcgacgccaa gacgctcgac tgcagtccgg agatgtgatt gttgttcctc cccttggtcc
     2161 tgtggccgcg atcagcggcg cagtcaagcg ggccgggatt tacgagctga aatcggggat
     2221 tcggttgacc gatctgctcc agctcgccag cgggctgact cctgcggcca atcatcagcg
     2281 atgccagctg ttccgccttg atccgggcaa ggggcgggtt atccttgacg tcgatctcaa
     2341 cgcggtcgtc gatacgacgg ggaaaaaacg gccaacgagc aacgggtccg atcttgtgat
     2401 tcaagatgga gattacgtcc gcgtcgggtc gttgccgacg caagtggcga acgtggtgac
     2461 gttagccgga gctgtgaaaa atcccggccc ctatgaatac aagcctggga tgggcatccg
     2521 cgatctgctc aagcccgatc agttgacagt ggacgcttat ctggacagtg cggaactcat
     2581 tcgcaccgat ccctttactt atcagacgaa agtgatctcg ttcagtccca aggcactatt
     2641 tgacagcgag aaaggcaacg acctgcctct ccagcgcatg gatcaggtcg tcgtgggtac
     2701 ccaaatgcgt cctcccaacg tggtgcttct ggagggcgag gtcaaacggc ctggtcattt
     2761 cacgttggaa accggcgaac ggctcagttc cgtgctgaaa cgggctggag gacttaccaa
     2821 caatgcattt ccggagggga ttatccttgt tcgggaatcg gttcgaaagc ggcagcaggc
     2881 tgaacttgat cgattcctgg cttccgaacg gcagcggctg accgcgcaat cggccagcta
     2941 tgcggccgga gctgtcggtg tgcctggcgg cgctgcgagc ggccaagcgg cggaacagca
     3001 agtgttggca ctgcgcctgc agcaactgga ggctgtggcc gtacggctgg agttgggtcg
     3061 tgtggtggtg aatgtccgat ccatcgagga gttggagggc accgaggacg atatcacgat
     3121 ggaagcgcgg gatcgcattg tcattccgca gccgccgaag acggttagta ttatcggctc
     3181 ggtgaagaat cccagcacgg tgatttaccg gacgggtctc aacttcgagg attatgtgca
     3241 acaagccgga gggctgaccg aggatgccaa caaacatgaa atgtatgtca tgagagccaa
     3301 tggctcgaca gaaggcgcgt atgtgaagat caaagagatg aggccgggcg atactgtcgt
     3361 cattccacaa aagatcgagg ccaagacacc gacgttagcc ttgtggcaga gcatcgccag
     3421 cattgtcggc agcgtggctt tggtggcagc ggccatttct gtaattggaa attaatgccg
     3481 gatgtatccc ttcacataac cgtttgccgt cgtctcacgt ctcttgctgt aatcggccca
     3541 gaaagacggg gcaaaccaat cagatgaatc aagccaacca gctaaaccct gcaatccaaa
     3601 acgaggaaga ggtcaccctg ctcgattatt ggcaggtcgt ctcgaagaga aagaagtcca
     3661 tcatcgcgtt ctgcagtgtg atggttggcg ccgctctcgt ggtgagcctt ctgcttccca
     3721 agatctatga atcgacggcc acgcttcttc cccagttgga atccaacacg ggatttggct
     3781 tgggtgccct cctgggatcc agtgcggctg gaagcgcggc gcaaagtttg gggatttcac
     3841 tgcctggcgc accggcgacg ccgacggata tcttcaccgc catgctcaaa tcgagaatca
     3901 tggcggatga ggtaattcgc caatttaact tgcaggaacg ttatgagaaa aaaacgatgc
     3961 aggaaaccag gctggcctta gaaaacgcga cccgcatcgt ggtcagtaaa gaaaaagtca
     4021 tcaagattac ggtcgaggat aaggaccctc aattggcagc tgatatcgcc aacttctatg
     4081 tattgaatct cgaccggctc aatcagacgt tgagcgtgag taaagcgagg gaaaatcgga
     4141 agttcatcga gcagcgtgtt gctgagacga aagatgcatt ggtgaaggtc gagaacgcgc
     4201 tcaaggaatt ccagacgcag aatcgaaccg tggccatcga ggcgcagtca aaggccatga
     4261 tcgaggcggc cgcgacaatc caagcccaaa tcatggcgca ggaagtccag ttgcaggtga
     4321 tggggtccta tttgtccccg gacaatcccg atgtggcgcg ggtgcaatcc agcatcaacg
     4381 agctgcggaa gcaactccgc ttcatggagt cgggaaaggg ggggaagga
//
LOCUS       DATIFR010000075        11166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1196960, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000075 DATIFR010000000
VERSION     DATIFR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196960"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VLD60_04115"
     CDS             <1..219
                     /locus_tag="VLD60_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSB44171.1"
                     /translation="NAVGAIFLFVGFLMLFLPGQGVLTMVIGVSMLDFPGKRKVEATL
                     IGQPTVLGVINSMREKFGKPPLILAPDP"
     gene            225..2885
                     /gene="polA"
                     /locus_tag="VLD60_04120"
     CDS             225..2885
                     /gene="polA"
                     /locus_tag="VLD60_04120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HSB44172.1"
                     /translation="MSTAESNRKTLYLIDGSAYIYRAFFALPALNNSKGLQTNAVYGF
                     TTTLMKIIREHQPDCLAVAFDEKGPTLRHEEFKEYKAQRPPMPDGMKAQIPYIHRVVE
                     AMNIPAVRQAGYEADDLIGTLARQAEGAGYDVVIVTGDKDMLQLLTPHIRIYDPVKNK
                     WSGEAECRERFGVEPARVVEVMGLMGDSTDNIPGVKGIGEKTAMKLIAQFGTIDELLQ
                     RVDEVTPERIKTLLIEQADNARLSRKLATIQTESPVEFHPESYRIKPPHSDQLADLLR
                     ELEFSTLLKAVQPAPRTSDAKADRSTTIVDNEASAQRFVSELPKGDPLGICCLLAGPP
                     GVRSEVRGIALSTGEKTAFIPIDVRTFMRPITALLHDQSRTKVVHDLKATLLAFHRIG
                     LTLAAPYMDTMIADYLLNPNRRDHQLETIALEVLGDRLGRGKQGQTTSQSLFEADTGS
                     QEEATEAAAVLARLAPALTERLAEQGSLNLLTDVEMPLVPVLAEIERNGFLLDVETLH
                     GLSKELERDLDRMMETIAGLAGGEFNINSPKQLATVLFEKLGLKPVRKTKTGYSTDED
                     TLTQLSTQHELPAQILNYRSISKLKSTYVDALPELVNPDTKRLHTSLNQTVAATGRLS
                     STEPNLQNIPVKGDYGLRIREAFIAPRGHELLCADYSQIEPRILAHLSQDPRLVAVFA
                     KGEDIHMATAMEIFGLPSSQITRDMRRAAKTVVFGIVYGISPFGLSQNIGVTQAEAKK
                     YIDTFFERFSAVRALMDRNIAEGKEKGYTTTILGRRRPIPELQSGDPVQRGFGERMAV
                     NSPIQGSAADLIKVAMINVHKRLHEDLPHTKMILQVHDELIFEVPEKELDEAKKLVKQ
                     EMEGVGKQLHLSVPLKVDLGVGKNWRAAHP"
     gene            2912..3172
                     /locus_tag="VLD60_04125"
     CDS             2912..3172
                     /locus_tag="VLD60_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44173.1"
                     /translation="MQHIRCHAAGIMAGASLLVAVTVLIAQWPLFTATAQEPKRFEYR
                     IVEVLPDTQNMQTKLTEFGANGWELVAVSMGNMTEPRLIFKK"
     gene            3545..4411
                     /locus_tag="VLD60_04130"
     CDS             3545..4411
                     /locus_tag="VLD60_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44174.1"
                     /translation="MIHAVELDRQTAGFVHQLEDQRKLEKNLMIERVRGTKEGVESVF
                     HDIKERVEALERLTGSVSTSYIAPIGWSQLIFTGADSVSSPNTIGFGDRDETLCKHDQ
                     IRYLEDTNYLRLSANPHYAEHVLPNKSAHARAGANPSSWDNLGYVHVGYSTLLTLPAS
                     LEPTTVSIMSRVNTLFNWVEVNAVGDEWYAHVAVTARLWATYDGEFRQTPPAQFVNLL
                     ATPKQVRTHQEIESLAPSAALAMQVDVSAGSGTTLSIFETIELVATTPNGHAYVDGVF
                     SWEPLAVQLREG"
     gene            complement(4469..4885)
                     /locus_tag="VLD60_04135"
     CDS             complement(4469..4885)
                     /locus_tag="VLD60_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44175.1"
                     /translation="MSDVSIVRRGWWKTTVTVSLILMLGQTVAEGASARAAKDASERN
                     KAVTAAARYLQGQGFTEPMLATESVENNRLAVEVDNKGWARMNKNQKMDFLEKVNGAV
                     LAASGGVAVDLHVSMNGSKVADSAFSAGQQVIRLLE"
     gene            complement(5156..6427)
                     /locus_tag="VLD60_04140"
     CDS             complement(5156..6427)
                     /locus_tag="VLD60_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44176.1"
                     /translation="MPIENNFQHDELSRKNPGERLTTAELATSMPPDSPAWIEAMARY
                     GSTLFHAGVRAIVFLHGAIHGTDLFGMQRLDEVGGLKRGYSRGVSGLDALLAAMREDS
                     NGIPLLPGGLKPPLANDDATKKLLDEQIGDAGNFTNANVDLFHNSINKTLAQPIVCQR
                     MLWASEHHHLGRALAAVRLLDALRQLVEQDRLTQGQRILLQAHGQAGLVLALASNLLC
                     PSQITGRPKLLGLLASYAEQTDQPELIATIKQIEPLLATSSLLNGAMLDVVTFGTPVR
                     YGWDPSGIGRLLHIVNHRNLRTDGKAWLAKMELPQLTMEMPIAWGGDYVQELAVAGSD
                     AVPKCEPAKVANKAIWEMVEPYDGFERWLECARRAVRFPSEGRCLLVDYKDSTGSTNP
                     RDHYYGHATYTRREAILFNTAQIVGCMQYSV"
     gene            complement(6473..7036)
                     /locus_tag="VLD60_04145"
     CDS             complement(6473..7036)
                     /locus_tag="VLD60_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44177.1"
                     /translation="MNQVNGYTVCMGLGVCLTLSPLPVRSADSGDVRSHAEQSFEQLK
                     RQTDHKGLTTSNAGPGHSTEERSSYSPDQYLIGKGQGDMAKGAITCQRVSELSARSDI
                     AKQIRILVKEHMVDRVRERTGRDAEQDIEVTREEIVQEYLQGVKIVDRQVDEDTKTCS
                     ATAVMSKSQIQSKPAPDHPEPAATITR"
     gene            complement(7033..7278)
                     /locus_tag="VLD60_04150"
     CDS             complement(7033..7278)
                     /locus_tag="VLD60_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44178.1"
                     /translation="MAKIRVVTSQGKHRMGDRDTNEDEVRLATKAAKRNALEQVATHL
                     ESITVGGGDITNDEIRIGGMVEVGAGVAVKEERDRAQ"
     gene            7599..8612
                     /locus_tag="VLD60_04155"
     CDS             7599..8612
                     /locus_tag="VLD60_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSB44179.1"
                     /translation="MAVGFWRLHASALGLCFVLLGTSSGAATPVPPADGAAPSSPPTE
                     HVILFVLEGFGQDSLKGGTMPTLNKLIKEGAVTWSATAVKPALRLPTMASLITGMPVE
                     KHGITWNAFEFSRGYPRAPSVFDYLDLSGGRDSAIFFMDESLYQLARPEPYTDYQMCG
                     PLKPECNPDRIVSYIQQYFKKATSGHGYGHAILSLPHFLVVHLPEAGRVGASRGWASK
                     EYREALRTVDKAMNSILDIYKAQGLLKRTTVFVTSLSAEGKDANQEHGGNGTPVVPWI
                     ASGVGIKHGYMIHQPVSIIDTGATVMRTLGLQTHTEWESRPVEEIFQFAFVAAAPNSS
                     LPQ"
     gene            8619..9062
                     /locus_tag="VLD60_04160"
     CDS             8619..9062
                     /locus_tag="VLD60_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44180.1"
                     /translation="MKFMNRRMWANVTRGSLLSLMGGTLLFSAAQAGPPAAYLTEHSI
                     TIDATVLEPPSWWSVPGVTPSIYNSDPESMDAHKTTDKRTLALKPGKYKFVSFTFDFP
                     FSVNLEGKLEFAPSLDQCVAGRGTQTLIVRCKRTYPHGGQPDYTY"
     gene            9078..10448
                     /locus_tag="VLD60_04165"
     CDS             9078..10448
                     /locus_tag="VLD60_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB44181.1"
                     /translation="MPMTPALDDLLEALEDVDDATREEAAKALAGIGDPKSLDALIGA
                     CSDEFWSVRAHAGCAVAKIGGPKAVEALIGLFNDPIMEVRNQAVEATARMGVGVVDRM
                     IAAMKDERWRVREHAAKTCGEIKDRKSVDALIVACRDRDGAVKSAAAEALGKIGDPKA
                     IPALIKLFRDTSKIVRETAGTALVYIGQPSVDPLIECFKDKDFVVRCHAARALGGMTT
                     DYQIGRTWVREPKVVDALIAALKDPDRAVREDATIALGMIGDPRAIDSLIEAMKDGAV
                     KRHAIASLGMIGDPRALPPVLDALKGKGIRQDGTPTPGCIVSEDAFIKEAAATALGQF
                     RDPRVIPDLIMLLKDGVLREKASAALATIGDTAIEPLIAFLYDPKASEVVGEGERVLS
                     YASVRLSAKDALRQLVMETLEKLGWTLPQDDLAVDSSSADNARVDRPLGDTGRFGPSG
                     DVAKSS"
     gene            complement(10569..>11166)
                     /locus_tag="VLD60_04170"
     CDS             complement(10569..>11166)
                     /locus_tag="VLD60_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44182.1"
                     /translation="TATPMKVVATLDNNQVHPAGCGGIESGGTMYINSGGGWPIAPLS
                     YDVYALDMTNLPKSISARLISQRDDKFADSHGMASVGRYVWSADRAGNNIEIIDTLSS
                     LSVGSVDLVTNANSDPAPDLMDVAPDGQYVFVGLRGPTPLTGNDKNANNAKGTIPGVG
                     VVHVDAGGKVGHYKGQAAITNMKDGKETADTHGIAVRK"
BASE COUNT         2305 a         3159 c         3362 g         2340 t
ORIGIN      
        1 aatgccgtcg gggcgatctt cctgtttgtc ggctttctga tgttgtttct tccaggacag
       61 ggcgttctca cgatggtcat cggggtgtcc atgctcgatt ttccgggcaa gcgaaaggtc
      121 gaggccacac tgattgggca gccgacggtg ttgggcgtca tcaacagcat gcgcgagaaa
      181 ttcgggaagc cgccgctcat cctcgcaccg gacccctgaa catcatgtcg accgccgaat
      241 ccaaccgcaa gacgctctat ctcatcgacg ggagcgccta tatctatcgc gcgttcttcg
      301 cccttccggc tctgaacaat tccaaaggcc ttcaaaccaa cgccgtgtac ggcttcacta
      361 ccacgctgat gaaaatcatc cgtgaacacc agccggattg ccttgccgtg gcgttcgacg
      421 aaaaaggccc gaccctgcgg cacgaagaat tcaaagaata caaggctcag cgccctccca
      481 tgccggacgg catgaaggcg cagattccct atatccatcg tgtcgtggag gccatgaaca
      541 ttcccgccgt cagacaggcc ggatatgaag cggacgatct gatcggcacc ttggcgcgcc
      601 aagccgaggg ggctgggtac gacgtggtaa ttgtcacagg cgacaaagac atgctgcagc
      661 tgctcacgcc gcacatccgc atctacgatc cggtcaaaaa caaatggtcg ggggaggcgg
      721 aatgccgcga gcggtttggc gtcgaaccgg cgcgtgtcgt cgaagtgatg ggactgatgg
      781 gcgacagcac cgacaacatt ccgggcgtga agggaatcgg tgaaaaaacg gcgatgaagc
      841 tgatcgccca gttcgggacg atcgacgaat tgctgcagcg ggtcgatgag gtgacgcccg
      901 agcggatcaa gacgttgctg atcgaacagg cggacaatgc gcgcctcagc cggaagctgg
      961 ccaccattca gactgagagt ccggtggagt tccatcccga gtcctaccgg atcaagcctc
     1021 cgcacagcga tcagctcgcc gatctgctgc gcgaactcga attcagcacg ctgttgaaag
     1081 ccgtgcaacc cgcgcccagg acttccgatg ccaaggctga tcgctcaacg acgattgtcg
     1141 acaacgaagc ttcggcgcaa cggttcgtga gcgaactgcc gaaaggcgat ccccttggca
     1201 tctgctgtct gcttgccggt cctcctggtg tgcggtcgga ggttcgcggc atcgcgctgt
     1261 cgacaggcga aaagaccgcc tttattccca tcgatgtccg gacgttcatg cgcccaatca
     1321 ccgcactcct gcacgaccag tcaaggacca aggtcgtgca cgatctgaag gcgaccttgc
     1381 tcgcctttca tcgaatcggc ctgacgttgg cagcccctta tatggacacg atgatcgccg
     1441 attacttgct caatccaaac cgtcgcgacc accaactcga aaccattgcg ctggaggtgt
     1501 tgggcgatcg actgggaaga ggaaaacagg gccagacgac ctcgcaatcg ctgttcgagg
     1561 ccgatacggg ctcgcaagaa gaagcgactg aagcggcagc cgtcctggcc aggcttgccc
     1621 cggcactaac cgagcggctg gcggaacagg ggagtctgaa cctcttaacc gatgtcgaaa
     1681 tgccgctggt gccggttttg gctgaaatcg agcgaaacgg tttcttgctg gatgtggaga
     1741 cgttgcacgg gctcagcaag gagctggaac gcgatctcga tcgcatgatg gaaacgatcg
     1801 ccggattagc cggcggcgag ttcaatatca attcgcccaa gcaattggcg actgtcttgt
     1861 tcgaaaaact cggcctcaag ccggtgcgta aaacgaagac gggctactcc acggatgaag
     1921 atacgctgac gcaactgtcg acccagcatg aactgccggc ccagattctg aactatcgga
     1981 gcatcagtaa gctcaagtct acctatgtgg atgcgctgcc ggagttggtc aatccggaca
     2041 cgaaacggct gcacacgtcg ctgaatcaaa cggtcgccgc aaccggccga ctgtcctcca
     2101 ccgagcccaa tctgcaaaat atcccggtga aaggcgacta cgggcttcgc atccgcgaag
     2161 cattcatcgc cccgagaggt catgagctgc tctgcgccga ctacagccag atcgagccgc
     2221 ggatcctggc gcatctctcg caagaccccc ggctggtggc cgtcttcgca aagggggaag
     2281 atatccacat ggctacggcc atggagattt ttgggctgcc gtccagccaa atcacgcggg
     2341 acatgcggcg cgcggcgaag acggtcgtct tcggcatcgt ctatggcatc agcccgttcg
     2401 gtctctccca aaacatcggc gtcacgcagg ccgaggccaa gaaatacatc gatacgtttt
     2461 tcgagaggtt ttcagccgtg cgtgcgctca tggatcgcaa catcgcggag ggaaaagaaa
     2521 agggctacac gaccaccatc ttgggccggc gccggccgat tccagaattg cagagcggcg
     2581 atccggtgca gcgcgggttc ggcgagcgca tggcggtgaa cagccccatc cagggctcgg
     2641 cggcggatct gatcaaggtg gccatgatca atgtccacaa gcggctgcac gaggatctgc
     2701 cgcatacgaa gatgattctc caagtccacg acgagttgat tttcgaggtg ccggagaagg
     2761 agttggacga agcgaagaag ctggtgaagc aggagatgga aggggtggga aaacaactcc
     2821 acctctcggt gccgttgaaa gtggatctgg gcgtcgggaa aaactggcgc gcggcgcatc
     2881 cataaccgaa cacacaacaa cgaggtgaat catgcagcac atacgttgtc atgcggccgg
     2941 gattatggcc ggggcgagcc tcctggtcgc tgtcacagtg ctcatcgcgc agtggcccct
     3001 gttcacagcg accgcccagg aaccgaaacg cttcgagtac aggattgtgg aggtgctgcc
     3061 ggatacccaa aacatgcaaa cgaaattgac cgagttcggc gcaaacggct gggagttggt
     3121 ggcggtttcg atggggaaca tgacggagcc gaggctcatc tttaagaaat aatgggcaag
     3181 gtaccttcag aataatttac tcgctgatga ttaatcagcg ggtcaaatat gatgacgcgg
     3241 ggcttgcaga aaatctggac ccgtttgaag ggggccgacc cacacctttc ggcaatgata
     3301 gctcatcctg cgggtcttcg ctggggttct taatcagcgg ttccccttga atatgtgatc
     3361 cgggggcgaa gctcttgatc tatccccgtt caaacctgtg cctctcgttg gtccctgcct
     3421 tcatctagtc tagatccaga gccagtttgc gcgggcttag aggtggcacc ccttttgcgg
     3481 tgatgcccat ggctttcatc attcatggga agccgaggtt gaaccataag aaaggggcac
     3541 catcatgatc catgctgtag agctcgatcg gcagacggcg gggttcgtcc atcaacttga
     3601 agaccagcgc aagctcgaga aaaatctcat gatcgaacgg gtgcgcggga cgaaagaggg
     3661 cgtggaatcg gtgtttcacg acatcaagga gcgagtcgag gccttagagc gcctgacggg
     3721 atcggtgagc acgagctaca tcgcgccgat cggctggagc cagttgatct ttacgggtgc
     3781 cgacagcgtg tcctcgccca ataccattgg cttcggcgac cgggacgaga ccctttgcaa
     3841 gcatgatcag atccgctacc tggaagacac aaactatctg cggctgagtg cgaatcctca
     3901 ctatgctgag catgtgctgc cgaataaatc agcccatgcg cgtgccgggg ccaatccctc
     3961 gagctgggac aacctcgggt acgtgcatgt cggctactcg accttgctga cacttcccgc
     4021 ttcgctcgaa ccgacgacgg tgtcgattat gtcgcgggtg aacacccttt ttaattgggt
     4081 ggaagtcaac gcggtgggcg acgaatggta cgcccatgtg gcggtaacgg cgcggctctg
     4141 ggcgacctat gacggggaat ttcgccagac gccacctgca cagttcgtga atcttctggc
     4201 gacgccgaag caggtgagga ctcatcagga gatcgagtcg ctggctccca gcgcagcgct
     4261 cgcgatgcaa gtggatgtct cggccggcag cggaacgacg ctcagcatct ttgaaacgat
     4321 tgaactggtt gcgacaacgc cgaatggtca cgcctacgtg gacggggtct tttcgtggga
     4381 gcctctggct gtccaattgc gcgaagggta aggggttcga aggaatcggc tcccctccag
     4441 gagagaaggg gagccgccga gtgtgagatt actcgagcaa tcggatgacc tgttgtcctg
     4501 cagaaaacgc tgagtctgcc accttgcttc cgttcatgga gacgtgaagg tcgaccgcca
     4561 caccgccgct ggcagccaac actgcgccgt tcaccttctc gaggaaatcc atcttctgat
     4621 tcttgttcat gcgcgcccag cccttattat ccacttcaac ggctaaccgg ttattctcca
     4681 cgctctcggt cgcgagcatt ggttcggtaa agccctgtcc ttggagataa cgagcagcgg
     4741 cagtcactgc cttgttgcgc tcgctcgcat ccttcgctgc acgggccgag gccccttcag
     4801 cgactgtttg gcctaacatg agtatgagac tgaccgtcac cgttgttttc caccatcccc
     4861 gccgaacaat tgacacgtcg ctcattcgaa gccctccatg ttcatgttga tgtttgataa
     4921 tgagacgcgg gcggaatgag caaacttcgg gccgcggatt tgcggctcgt tggtttaggg
     4981 aggggagctg ctccaacgaa aatccaaggg tttccgtcac ccgtattcag aaccgttctc
     5041 gtcaaacgat ttaccttccg aaaaatgaca gtgtccgaca gaaaatgttc gcgactttca
     5101 gcgggcgatg ggacaaatga gcgtccgagg gagataggga aggtgagact gctgcttaca
     5161 cagaatactg catgcaaccg acgatctgag ccgtgttgaa gagtattgct tcgcggcggg
     5221 tgtaggttgc atggccgtag taatggtcac gcggattcgt ggagccggtc gagtccttgt
     5281 aatcgactag caggcagcga ccctcgctgg gaaagcgcac ggctcgccgc gcacattcca
     5341 accatcgttc gaatccatcg tacggttcga ccatttccca aatggctttg ttggcgacct
     5401 tggccggctc acatttgggc accgcatcgc ttccggcgac ggccagttcc tgcacgtagt
     5461 cgccgcccca ggcgatcggc atctccatcg tgagttgcgg caattccatc ttcgccagcc
     5521 aggctttccc gtcggttcgg agattccggt gattcacgat gtgcaatagc ctgccgatgc
     5581 ccgagggatc ccagccgtac cggaccggtg tgccgaatgt caccacatcc agcattgctc
     5641 cgttcaacaa tgaggatgtg gcaagcaacg gctcgatctg tttgattgtg gcgatgagct
     5701 ccggctgatc ggtctgttcc gcgtaggacg ccagcaggcc gagtagcttg ggcctgccgg
     5761 taatctgact ggggcacagc agatttgagg caagtgccag gaccaggccg gcttgcccgt
     5821 gggcctgcaa gagtattctc tggccctgag tcaggcgatc ttgttcaaca agttgccgga
     5881 gggcatcgag caggcgaact gcggcaagag cacggcccag gtggtggtgt tcggacgccc
     5941 agagcatgcg ctggcagacg atcggttgcg ccagggtctt attgatcgag ttgtgaaaaa
     6001 gatcgacgtt cgcgtttgtg aaattccctg cgtcgccgat ctgctcgtcg agtaatttct
     6061 tcgtcgcatc atcgttcgcg agcggcggtt ttagcccgcc gggaagcagc gggatgccgt
     6121 tgctgtcttc ccgcatggcg gcgagcaagg cgtcgagtcc cgaaaccccg cgcgagtacc
     6181 cgcgcttgag gccgccgact tcgtccagcc gctgcatccc gaacaggtcg gtgccgtgga
     6241 ttgcgccgtg cagaaagaca atggcccgaa ctccggcgtg aaagagcgtt gacccgtatc
     6301 gggccatggc ctcaatccag gcgggggagt ccggcggcat actcgtcgcc agctcagcgg
     6361 tggtcagccg ttcaccggga tttttccgcg agagttcgtc atgctgaaaa ttattctcga
     6421 tcggcatggc gggggattct tacccgaaag cgcgagaaga gtgcaactgg acctagcggg
     6481 taatcgtggc ggctggctca ggatggtctg gagccggttt tgattggatc tgactttttg
     6541 acatgactgc ggtggcggag caagtcttcg tgtcctcgtc aacctggcgg tcgacgattt
     6601 ttacaccttg cagatactcc tgaacgattt cttcacgtgt gacttcgatg tcttgctcgg
     6661 catcgcggcc ggtccgttcg cgaacgcggt cgaccatatg ttccttcacc agaatgcgaa
     6721 tctgtttggc gatatcgctg cgggcagata gttccgacac ccgttggcag gtaatcgcac
     6781 ctttagccat atcaccctga cctttaccga tcaggtattg gtccggagag taactgctcc
     6841 gctcctctgt gctgtggcct ggtccggcgt tcgacgttgt cagacccttg tgatcagtct
     6901 gtcgcttcag ctgctcgaaa gactgttcgg catggctgcg aacatcgcct gagtcggccg
     6961 agcgtaccgg gagaggtgaa agggtaaggc agactccaag ccccatgcag actgtgtatc
     7021 catttacttg gttcattgcg ctctatctct ctcttccttc accgccacac ccgctcccac
     7081 ctccaccatg cctccgatac ggatttcgtc attcgtaatg tcgccgccgc cgaccgtgat
     7141 gctttcgaga tgggtcgcga cctgttccag cgcatttcgt ttggctgctt ttgtggccag
     7201 gcgaacctca tcttcatttg tatcgcggtc acccatgcga tgtttgccct gtgacgtgac
     7261 gacgcgaatc tttgccatag ccggtaaaac acagaacagc cttgtaatgg taacgagggg
     7321 cggctgacgg gatgacggga acatcctccg actggcctga cgcggcggca tcatccgtga
     7381 ctccgtcggg catcgccttc accctctcca tgaggactga cgcatcagcc accagttggt
     7441 tgacagccgg ccggatgaac cacgcaggtt gagcatagca cccgccaaac cgtttcgcca
     7501 gccgctccgc gggacctcag ggctggcttg ccttccagaa aattcgcgtg ctaaggtaca
     7561 aattccctat ggtaattcaa aggaaaagag ggtgttctat ggcggtcgga ttctggcgtc
     7621 ttcatgcgag tgctcttggt ttgtgcttcg tgctcctcgg aacctcgtcc ggtgcagcca
     7681 ccccggttcc accggccgat ggcgcagctc cctcatcccc tccgacggag cacgtgattc
     7741 tttttgttct ggaaggattc gggcaggact cgctgaaggg cggcacgatg ccgaccctca
     7801 acaagttgat taaagagggg gccgtcacat ggtcggcgac tgccgtgaag ccggctctgc
     7861 gcttgccgac gatggcctca ttaatcaccg ggatgccggt tgaaaagcac ggcatcacgt
     7921 ggaatgcctt cgaattcagc cggggctacc ctcgggctcc cagtgtgttc gactatctgg
     7981 acttaagcgg cgggcgtgac agcgcgatct tcttcatgga cgagtcgctg tatcaattgg
     8041 caagaccgga accctacacg gattatcaga tgtgcggtcc gttaaagccg gaatgcaatc
     8101 cggatcggat cgtgtcctac attcaacaat atttcaagaa ggccaccagc ggtcatggct
     8161 acggacacgc cattctctct ctgccgcatt ttctcgttgt gcatcttccg gaagcggggc
     8221 gagtcggggc ttcgcgagga tgggcatcca aagagtatcg agaggcgctg cgcacggtcg
     8281 ataaggcaat gaattcgatc ctggacatct ataaagccca agggttgctg aagcgcacca
     8341 ccgtgttcgt gacgtcgttg agcgccgaag ggaaagatgc caatcaggaa catgggggaa
     8401 acggtacgcc ggtcgttcca tggatcgcct ccggtgtcgg catcaagcac gggtatatga
     8461 tccatcagcc cgtgtcgatc atcgataccg gtgcgacggt gatgcgcacg ttggggttgc
     8521 agacccatac cgaatgggag agtcgtccgg tggaagaaat tttccagttt gcctttgtcg
     8581 ccgcggcgcc gaattcgagt ctgccgcagt agagtcgtat gaagttcatg aatcgtcgta
     8641 tgtgggcgaa cgtgacgcga gggtcgctct tgagcctgat gggcggtacg ttgctctttt
     8701 cagcggccca agccggaccg ccggcagcct acttgacgga acattcgata accatcgatg
     8761 cgacggtgct ggagcctccg tcatggtggt cggtgccggg tgtcacgccg tcgatctaca
     8821 attctgatcc ggaatcgatg gacgcccaca agacgaccga taaacgcacg cttgccttga
     8881 aaccgggcaa gtataaattt gtgagtttca ccttcgattt tcccttctcg gtgaatctcg
     8941 aaggcaagct cgaatttgcc ccctcgcttg accaatgtgt ggcgggtcgc gggacacaaa
     9001 cgttgatcgt tcgctgcaag cgaacctatc ctcacggcgg gcagccggac tatacgtact
     9061 aatgaaatga ggggacgatg cctatgacgc cggctctgga cgatttgcta gaggcactcg
     9121 aggatgtcga cgatgcgacc cgtgaagagg cggcgaaggc tctggctggc atcggcgatc
     9181 ccaagagcct ggatgcattg atcggcgcct gcagcgacga attctggtct gtgcgtgccc
     9241 atgccggctg cgcggtcgca aagatcggcg gtcccaaagc cgtcgaagcc ctgatcggcc
     9301 tgttcaatga tcccatcatg gaagtgcgca accaggcggt tgaggcgaca gccaggatgg
     9361 gggtcggtgt ggtggaccgc atgattgcgg cgatgaaaga tgagcggtgg cgtgttcgag
     9421 agcatgccgc aaagacctgc ggagagatta aagatcggaa gtcagtcgat gcgctcattg
     9481 tcgcctgccg ggatcgcgat ggtgccgtca aaagcgcggc ggcggaagcg ctggggaaga
     9541 tcggcgatcc gaaagccatc cctgcgttga tcaagctgtt ccgggacacc tcgaagatcg
     9601 ttcgagaaac cgcgggcacg gcgctcgtgt atatcgggca gccgtcggtc gatccgctga
     9661 tcgagtgttt caaggacaaa gatttcgttg tccgttgcca tgcggcccga gctcttggcg
     9721 gcatgaccac cgactatcag atcggacgta catgggttcg ggagcccaag gttgtcgatg
     9781 ccctgatcgc agcgctaaag gatccggacc gcgcggtgcg cgaagatgcc acgatcgcgc
     9841 tggggatgat cggcgacccg cgcgccattg attccttgat cgaggccatg aaggacgggg
     9901 cggtcaagcg gcacgccatt gcgtcgctgg ggatgatcgg cgatccacgc gcattgcctc
     9961 ccgtgctcga cgcgttgaag ggaaaaggca ttcgccagga tggcacgccc actccgggat
    10021 gcatcgtcag cgaggatgcg tttatcaagg aagctgcggc gacagcgctg gggcaattcc
    10081 gagatccccg cgtcattccg gacctgatca tgctgctcaa agacggtgtg ttgcgggaaa
    10141 aagccagcgc cgcgcttgcg acgatcggcg ataccgccat cgagcccttg attgcattct
    10201 tgtacgaccc gaaggcttca gaggtggtgg gtgaagggga acgggttcta tcgtatgcct
    10261 cggtgcgtct cagtgcaaaa gatgcattac ggcagctcgt gatggagacg cttgaaaagc
    10321 tcggctggac attgccacag gacgatctgg ccgtcgactc cagctctgcg gacaacgcgc
    10381 gcgtggatcg gccattaggg gatacaggcc ggttcgggcc gtccggcgac gtggccaaat
    10441 caagttgacc ggtcaggcgc cccttgctac gaagctgtcg gccttcagct ttcagccgga
    10501 ggcggcaaag tgccccacgt gatctttact ttctcatctg ctgattgctg accggggata
    10561 gctgcttgct acttcctcac agcaatcccg tgagtgtccg ccgtttcttt tccgtccttc
    10621 atgttcgtga tggcggcttg gcctttgtag tggccgacct tcccgcctgc atccacgtgg
    10681 acaacgccca caccgggaat cgtgcccttc gcgttgttcg cgttcttgtc atttcctgtt
    10741 agcggagtcg ggccccgtaa tcctacgaac acgtactgtc catccggcgc gacgtccatc
    10801 aggtcagggg cggggtcgct attcgcgtta gtgacgaggt ctaccgagcc gacactcaag
    10861 ctgctgaggg tatcgatgat ctcgatattg ttgccggcgc ggtccgcact ccagacatag
    10921 cggccgaccg atgccatgcc atgggaatcg gcaaatttgt catcccgttg tgaaatgagt
    10981 ctggccgaga tcgacttcgg caaatttgtc atgtcgaggg cataaacatc gtacgacagc
    11041 ggggctatcg gccatccgcc gccggaattg atgtacatcg tcccgccgga ttcgattccg
    11101 ccgcagccgg ccgggtgcac ttggttatta tcgagcgtgg ccacgacttt catcggggtt
    11161 gcggtg
//
LOCUS       DATIFR010000076         1533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2153666, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000076 DATIFR010000000
VERSION     DATIFR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2153666"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1422
                     /locus_tag="VLD60_04175"
     CDS             253..1422
                     /locus_tag="VLD60_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSB44183.1"
                     /translation="MNAGRTVFSQLIEHVPSKEFQKCVARYQGDYYLKRFSCWDQFLA
                     MAFAQLTYRESLRDIEACLRAMSGKLYHMGFRSKVARSTLADANESRNWRIFADFAQA
                     LIAIARPLYARDPIGVDLDQGLYALDSTTIDLCLSLFPWAKFRKHKAAVKMHTLLDLH
                     GNIPTFIRITDGKVHDVNILDEIFPEAGAFYVMDRGYIDFERLYVFTLCSAFFVVRTK
                     EKIVLQRRYSHPVDKTTGVRSDHIVVLAAIESAKAYPDPLRRVSYFDAETNTRLKFLT
                     NNFVLPALTIARIYKCRWQVELFFKWIKQHLRIKAFYGTSQNAVKTQIWIAVSVYVLV
                     AIVRKRLGLEASLYQILQILSVTLFEKTPILRALQAPDSEDDLGESGKQLILFDF"
BASE COUNT          336 a          443 c          442 g          312 t
ORIGIN      
        1 aaaacctttg tataacacct cggcggtaat gaccgcggtc gggacttgga taaaaggacc
       61 gcccggagtg aatgggctgg cgtagacaac gtgcacattg cagttcactg ctgtctgggg
      121 aaaatgggca cggcgtctga caaccctcga aggatcagtg gaatagatgc tgctcggttt
      181 gggaacgatt tgaaatgcga ccgcccgaaa gggagggcct gatcgcgagt ttaggatgcg
      241 gaacaatggt tggtgaacgc cggccggacc gtcttctctc aactcatcga gcacgtgcca
      301 agcaaggaat tccagaagtg cgtagcgcgc taccagggcg actactacct gaagaggttt
      361 tcctgctggg accagtttct ggcgatggct ttcgctcagt tgacttaccg cgaaagcctg
      421 cgcgacatcg aggcatgtct gcgcgcgatg agcgggaagc tctaccacat gggattccgc
      481 agcaaagtgg cgcgctccac gctggccgat gccaacgagt cccgcaactg gcgcatcttc
      541 gcggatttcg cgcaggcctt gattgccatc gccaggccac tgtacgcccg cgatccgatc
      601 ggagtggatc tcgaccaggg cctgtacgcc ttggactcca cgaccatcga cctttgcctg
      661 tcgctgtttc cctgggccaa gttccgcaag cacaaggccg ccgtgaagat gcacacgctg
      721 ctggacctgc acggcaacat ccccacgttc atccgcatca cggatggcaa ggtgcacgac
      781 gtcaacatcc tcgacgagat ctttcccgag gccggcgcgt tctacgtcat ggatcggggc
      841 tacatcgact tcgagcgtct gtacgtgttc acgctgtgct cggccttctt cgtggtgcgc
      901 acgaaggaga agatcgtgct ccagagacgc tactcgcatc cggtggacaa gaccacgggc
      961 gtccggtccg accacatcgt cgtgctggcc gccatcgagt cggccaaggc atacccggac
     1021 ccactgcggc gtgtcagtta cttcgatgcc gagaccaaca cacggctgaa gttcctcacc
     1081 aacaacttcg tgctgcccgc acttacgatc gcccggatct acaagtgccg ctggcaggtg
     1141 gagttattct tcaaatggat caagcagcac ctccggatca aggccttcta cggcaccagc
     1201 cagaacgcgg tgaagacgca gatctggatc gctgtgtcgg tttacgtgct ggtcgccatc
     1261 gtccggaaga ggctggggct ggaagccagt ctttaccaaa ttctacagat tctcagcgtc
     1321 acgcttttcg agaaaacgcc cattttacgg gcactccaag cgcccgactc cgaagacgat
     1381 ttaggcgaga gcggcaagca gttgattctg ttcgactttt aaccggacag tagtggttcg
     1441 cgaaaaaatg aagccgcgtc cttggaaggt caagaagtac aggatcgacg gacggacctt
     1501 agaacggtgg tttttgaaaa ccctgatcaa tct
//
LOCUS       DATIFR010000077        12026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_432266, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000077 DATIFR010000000
VERSION     DATIFR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432266"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VLD60_04180"
     CDS             complement(<1..1447)
                     /locus_tag="VLD60_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSB44184.1"
                     /translation="MPPSIHPVDLITALRQKHLTPAIVFLTSRRACDEAMDSFDHTNS
                     ILPPARQEAIAKVLEQVIAQYPSIAEHPLIPTVQRIGVAAHHAGHLPSWKIAVEELMR
                     HGCLDAVFATTTLAAGVDFPARTVIITQSSIRKARDFADLTIGEVQQIAGRAGRRGKD
                     LVGFAIVTPSPYIDLGVLTKGFTGHPEAINSQFVISYPMVLNLLKAHPLDQMQPILAK
                     SFAQFQLNQRAEILERKLDDLHQQMEPYGPRVCTDWISQWHLFDQVRRQKSHRAPLRK
                     HEPPEVAARFHFLTPGRIAGLAKNRGVVLRQYRSKGQKSPMVTLLRSGGAITECPAAI
                     VTEVYDRVLDCTEAPAYPWATSESLDTIMRELEDLPTRLPVLPILVPKDQEALPDAIV
                     QTLGDFSCPTCPSRSACQKDHAHASKLRQEQHRHIKTIQALRTSLWHRFQEKIDALQH
                     FGYLSPTTHLTEAGEWARLIRIDHSLLITELI"
     gene            complement(1422..2660)
                     /gene="glgC"
                     /locus_tag="VLD60_04185"
     CDS             complement(1422..2660)
                     /gene="glgC"
                     /locus_tag="VLD60_04185"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSB44185.1"
                     /translation="MNNIFTMVLAGGRGERLHPLTEHRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRKVAVLIQYKSHSLDRHIRAGWDVLNAELGEYIASIPPQQRISEEWYRGTAD
                     AVYQNLFLLDNEQADYVLVLAGDHIYKMNYMEMFHWLVAKNADVVVGALDIPIEEASR
                     FGVMSVDEEFRVTEFNEKPASPTPIPGDPSKAFASMGIYLFRTQSLREELMADAQEGT
                     AHDFGKNIIPRMIAARRVFTFKFQDANKKAVKYWRDIGTLDAYWEANLDLVAVDPLFN
                     LYDQEWPIRTYQGQFPPAKFVFAQDYQGGRMGVALDSIVCGGCIISGGRVQTSVLSPN
                     VRVQDHADVRESVIMENVVIGEHCRIRRAIIDKDVIIPPKMEIGYDREADAQRFTVTE
                     SGLVVISKGMKLNASLDTSG"
     gene            2787..3089
                     /locus_tag="VLD60_04190"
     CDS             2787..3089
                     /locus_tag="VLD60_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44186.1"
                     /translation="MKQDVVVSSLAKKSVMLPKKKAAPESQEALLWRLEHLESQMQKL
                     SELVRDHAEDVDRLVRIVAEDRNAIRQQLLRKHHEQVRVRKHLRASNSNSKVGLDF"
     gene            3467..4732
                     /locus_tag="VLD60_04195"
     CDS             3467..4732
                     /locus_tag="VLD60_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB44187.1"
                     /translation="MFSVFPPASAAPSPAPPAGEENGAAAKALRLSLNEAIGLFIKQN
                     IDVLVAKFGIEYSKGQEITAALFPNPVATIGTLSSFTQGRTASNSGALFGQVQQLFEL
                     AGKRGYRIESSAYGTQSSEAAFEDAVRQLGFTVKDTYYRIQLAQRRLTLAEENRDRFA
                     RILDINTIRFKKGYIAEVDLIRIRLQMVDFQSQVIQSLQEAETARGDLRQLLRLSPKI
                     TLELTTDLDFRRIDPDISKLRTVALDVRPDIKARRFTYSQRESDLKLAKAYRVPDLTV
                     GGGYTIQGAQGPDNPQQWALNLGLPLPLFNRNQGGIRQAEVAVQTAEADLNKTLNLVE
                     NQVEVAYRNLLQSRRLVEAYVGGVLEDARATFTIVERAYERGGATILDLLDAARTSRT
                     IQQNYIEALFNYQRNLLQLESAVGQEIAS"
     gene            4729..5877
                     /locus_tag="VLD60_04200"
     CDS             4729..5877
                     /locus_tag="VLD60_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB44188.1"
                     /translation="MMRGTPSAWITILLIASVGCGKGDQPGNSDAATPPAKRAVISTD
                     IGSRVETGLVDYSGSQQDLSLAGKVAYGEDRYSKISSPLQGRVVEVRVHLGDRVKAGD
                     VLMVVDSPDIAQAYSEYVKEDSDLQYATRAHELAKDLYENKAMPLKDLKQAENELVKA
                     RAEFRRAKERLLSLRIPAEELSKPLDKQKITSRFEMKSPLTGIVVERSVTPGQSVSGD
                     SDHVLFTVADLDMLQVVADVYERDLALVKEGQFAMVKVEAYPDVDFPATVASIGDVVD
                     PASRTIKLRAWVNNQDHRLKPEMFARLHIQIGDATRILVVPKEAVLEVDGRQFVYIVE
                     DSNRYVKREVKATNFSPDQMRVLEGLVPGQRIVIKGAVLIKGQEVKGG"
     gene            5923..8988
                     /locus_tag="VLD60_04205"
     CDS             5923..8988
                     /locus_tag="VLD60_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSB44189.1"
                     /translation="MIARVVEISLVQRIMVCALGFALLFGGLYAFHLLDIVAYPDPSP
                     PMVELITQNPGWSSEEMERQITIPLEVALTGMPGLTDIRSLSIFGLSDIKVYFDFGTD
                     IFRDRQEVLNRIASVELPQGVQPQLSPWWAIAEIYRYELTGDPGVSLTDLKTIQDWQV
                     RRSFRRIPGVIDVTGFGGTTKEYHVDIDPGKLISYGVNLSQVMTALQKSNANVGGNYL
                     ALGAQNYNIRGLGLINSIQDIENVMVVQKEGTPIFVKTLGQVKVGNRVRLGKVGIDDR
                     DDVVEGVVLLQRGYKALPVLDKVRQKVDDLNTWKLPPGVKVRTFYDRTALINTTVETV
                     TDILISGMVLVFVLLYVFLGNIRAAGIVALTIPLSLLFTFSMMVVIGQSANLISLGSI
                     DFGIIVDATLIIVESIFFNLSHGKTPGLTVHQQIVRAARQVGQPIFYSTTIIVVAFIP
                     LFTMTGVPGKIFAPMSITYGFALLGALLMAFTLAPVLCSFLLKGHIKEEDTAVVRPLR
                     RLYHGTLEWALDHRKSVVMFACMLLVVALIALQFMGGEFMPALEEGNLWVRATMPVDI
                     SFDQAARLTTDIRAMFRESPEVTTIVSQLGRPDDGTDPTSFFNAEFLANLKPQAEWRK
                     GLTKDALIEEIEGKLRTIPGVIFNFSQVIQDNVEEAMSGVKGENSIKLFGTDLKVMEA
                     VAVEIEKEMSQIRGVKDLGIFRLVGQPNLLIKVDREASARYGLQVSDVNAVVQAAVGG
                     QAVTQVYEGERLFDLVVRFLPEYRQDMESISNILVSTPDGARIPLKQLATITTQTGAF
                     IIYRENNERYIPIKFSVRDRDLQSTVEEAQAVLAKKVKLPERYRMEWAGQYDQLKDEQ
                     NRLAKVVPVSLLLILFLLYTTFDSLKNALLVLSAVPFALIGGIVALVATHTHFSISAA
                     VGVISTLGVAILGGVLLISRIEDFRQTGLDLREAVVKAADVQMRPILMATLGAAIGLL
                     PAALATGIGSQAQKPLARVVVGGMLTAAFLILVVLPVLYEMVHRRAGKH"
     gene            9001..9804
                     /locus_tag="VLD60_04210"
     CDS             9001..9804
                     /locus_tag="VLD60_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB44190.1"
                     /translation="MVRYAYGWMVVAVVAVVTAGAGSARADSLGTGRVFPVQMPYEMP
                     PKGEDTPAVSVPQNTKPLSPEELQRAEALLPLLEGKQEFWAMGEFVHLGEPSVPVLVK
                     ALTMPGPRIRYNAIETISMLHAVSAVPALITTAKEPNEIPRVREHALRVAIRLDPSKS
                     PEAIQVMSKDPNSSIRKAAAFEARYVREKTVIPILIDMIDDDERFVALSAVQSLWILT
                     LHETEFHDWDASSKQDRQEWMAEWTEWWNGQKDTFQIPEPKRRSQGRQG"
     gene            9899..10429
                     /gene="def"
                     /locus_tag="VLD60_04215"
     CDS             9899..10429
                     /gene="def"
                     /locus_tag="VLD60_04215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSB44191.1"
                     /translation="MAVLPIAKLGNPVLRKISSAVDPRDIRTREFQRFIDDMFETMLD
                     EPGIGLAAPQVSRSIQLIVMGCEGEGGFPTTVLINPKIVYYGPQQAENWEGCLSVDGL
                     RGKVTRPSVVRVQALDRDSKPVDIEADGLYAVCIQHEMDHLIGKLFLDRMADLSTLTQ
                     LTEFDKYWRKEPTPVI"
     gene            10452..>12026
                     /locus_tag="VLD60_04220"
     CDS             10452..>12026
                     /locus_tag="VLD60_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44192.1"
                     /translation="MKTLLRVLQYLRPHRAMVVGTFVFAGLTTAFELVPPWLIKIVID
                     DVIQAGKTQLLAVVFLGLCGAYVLRNVCASTRIKLNNSLEQQIVHDLHVQVFGALQRL
                     SISFFENRPTGEVMSRVLNDTEHMQRIFVDGLEEIITAGLTLVGIMTVLFWLNWKLAL
                     LALVPIPILVVGAVAFTGRIHGHYRKIRKGSAELNALLQDSLAGIRETMGFNRQPYEE
                     DRFGRKSDQCRRDTLKAMYLWSYYSPGMMLIGSLGAALVLWHGTQEVLQQHLSVGELV
                     MFVSYLGLFYVPINQIHSVNHMLQHALAASERVFEVLDIVPDVKDQPGVRPPVPRLRG
                     EIEFHRAGFHYRNEAPILRDVSLFVKAGERVALVGPSGAGKSTTLKLMTRFYDVTEGA
                     ITIDGYDIRQMPLAFLRSQIGLVQQEPFLFNGTVRENILYGNLSASQAEVEAAAKAAR
                     AHEFIVTLPEGYDTWIGERGVKLSVGQRQRVSIARVLLKDPPIVMFDEATSNIDTETE
                     VKIREALDELTRGRTTI"
BASE COUNT         2593 a         3120 c         3574 g         2739 t
ORIGIN      
        1 ggatcaactc cgtaatgagg agagaatgat cgatgcgaat gagcctggcc cattcaccgg
       61 cttcggtcag atgggtggtg ggcgacaggt aaccgaaatg ctgcaacgca tcgatctttt
      121 cctggaagcg atgccagagg ctagtgcgaa gagcctggat tgtcttaatg tggcgatgct
      181 gttcctggcg cagcttcgat gcatgggcgt gatctttctg gcaggccgaa cgggacggac
      241 aggtcggaca ggaaaaatcg cccagtgtct gtacgatggc atccggaagc gcttcctgat
      301 ctttcggcac caggatcgga agaacgggaa ggcgtgtcgg aaggtcctcc agttccctca
      361 taatcgtgtc aagactttcc gacgtagccc agggataggc cggagcttcc gtacaatcca
      421 atacccggtc gtagacctcg gtcacgattg ccgcgggaca ctcagtgatc gctccccccg
      481 agcggagcag ggtcaccatc ggactctttt ggcctttgct gcgatactgc ctgagtacga
      541 ctccacgatt cttcgcgagc cctgcaatgc ggcccggcgt caaaaaatga aatcgtgccg
      601 ccacttccgg aggttcgtgc tttctcaggg gggctcgatg ggatttctgg cgacggactt
      661 ggtcgaacag gtgccattgg ctgatccaat cggtgcagac gcgcggacca taaggttcca
      721 tctgctgatg caggtcatcg agtttgcgtt cgaggatttc ggcccgctgg ttgagttgga
      781 actgcgcaaa gcttttggcc aggatgggct gcatctgatc caacggatgg gctttgagga
      841 gattcagtac catcggatag ctgatgacga actggctatt gattgcttcg ggatggccgg
      901 tgaagccttt ggtgagaact ccgagatcga tatacggaga aggagtgacg atggcgaaac
      961 ccaccaggtc ttttccccgg cggccggcgc ggccggcgat ctgctggact tctccgatcg
     1021 tcaaatcggc aaaatccctg gccttacgaa tactggattg tgtaatgatg accgtgcggg
     1081 cgggaaaatc gacgccggcg gccagtgtcg ttgtggcgaa cacggcgtcg aggcaaccgt
     1141 ggcgcatgag ttcttcgacg gcaatcttcc atgagggcag gtgacccgcg tggtgagccg
     1201 ccacgccgat tcgttgaacc gtgggaatta gaggatgttc cgcgatgctc gggtattgtg
     1261 cgatcacttg ctccaggact ttggcgatcg cctcttggcg ggctggcgga agaatggaat
     1321 tcgtatggtc gaacgagtcc atcgcctcat cgcaggcgcg gcgtgacgtc aagaacacga
     1381 tggccggggt caggtgcttc tgccgaaggg cggtgatgag gtcaaccgga tgtatcgagg
     1441 gaggcattca atttcattcc cttcgaaatg acgaccaggc ctgattcagt gacagtaaac
     1501 cgctgggcgt cggcttcgcg gtcataaccg atttccattt taggcgggat aatgacatcc
     1561 ttatcgatga ttgcccgcct gatgcggcaa tgctcaccga taaccacgtt ttccatgatg
     1621 acggattccc gcacgtcggc atgatcttgc acgcggacgt tgggcgaaag aacggatgtt
     1681 tgcacgcggc cgccggagat gatgcagcct ccgcacacga tggagtcgag cgccactccc
     1741 atgcgtccgc cctggtaatc ctgagcaaac acgaatttag caggagggaa ttgcccttga
     1801 taggtacgaa tcggccattc ctggtcatag agattaaaga ggggatcgac cgcaaccagg
     1861 tccaagttgg cttcccagta ggcgtcgagt gtcccgatgt cacgccaata cttcacggct
     1921 ttcttattgg cgtcctgaaa tttgaaggta aagactcggc gcgcagcgat catacgcggg
     1981 atgatgtttt tgccaaagtc gtgggcggta ccttcctggg cgtcggccat taactcttcc
     2041 cgcaggctct gtgtacggaa cagatagatg cccattgaag cgaaggcctt gctgggatct
     2101 cccggaatag gagtggggct ggccggtttt tcattgaatt ccgtgacccg gaattcctcg
     2161 tccacgctca tcacgccgaa tcgacttgct tcttcgatag ggatgtcgag ggcaccgacc
     2221 acgacgtccg cgttcttggc gacgagccag tgaaacattt ccatgtaatt catcttgtag
     2281 atgtggtcgc cggcgagaac gaggacgtaa tcagcctgct cattgtcgag gagaaacaga
     2341 ttctggtaca cggcatcggc ggtgccgcga taccactctt cgctgatgcg ctgttggggg
     2401 gggatcgacg cgatgtattc accgagctcg gcgttcaaca cgtcccagcc ggcacggata
     2461 tgtcgatcga gcgaatggga tttgtattga ataaggacgg cgactttgcg gagcccggag
     2521 ttgagacagt tgctcaatgt aaaatctata atacggtact tcccgccgaa aggaacggcc
     2581 ggtttagccc ggtgttccgt caggggatgg agccgttcgc cccggcctcc tgccagcacc
     2641 atcgtaaaaa tgttattcat gggcgggatt ttagcaggac ccccgggaaa tagaaagaaa
     2701 gcgtatccgt tgacttccga gagggggcag ccgatactac gggtacacta gagagagaat
     2761 tgaggagcgt catcaaagga gttggtatga agcaggacgt cgtagtttcc tcgttagcaa
     2821 agaagagcgt catgttgccc aagaagaagg cggctccgga gtctcaagag gccttgttat
     2881 ggcggttgga gcacctggag tcccagatgc agaaactatc ggaattagtc cgcgatcatg
     2941 cggaagacgt cgatcgcctg gttcggatcg tcgccgaaga tcgcaatgcc atccggcaac
     3001 agctactgcg caagcatcat gagcaggtac gggttcgaaa acatcttcgc gcgtccaatt
     3061 ctaattctaa ggtagggctc gatttctgat cgcctggacc tctggcactc ctcgctccca
     3121 ttgcatcatc cgccgatcgc ctttccgatt cgagcaatcc aagttattca atgacctgta
     3181 ttctatcacc tgatcgctgg tcataaatcc gtcgtgctga cggttccctt cgccccggtc
     3241 tttatgcctc attccatatc ggcagccctg tacagtctaa ctgcctgttt ctcaagagat
     3301 tatttgcatc gggggtagga cactgtataa tgcggatatt ccttgaggac gatgaggtgg
     3361 gttccattat cgcctccagc cgcattgagt ttcgtgcgag ggaccatacg tgcttgcgca
     3421 ttattcgcga cgatcgttct tcttctgcat cctactgatt agcctattgt tctcggtatt
     3481 ccctccggct agcgcggccc cttccccagc ccctccggct ggtgaagaga atggagcggc
     3541 agccaaagcc cttcgtctca gcctcaacga ggccatcggg ttattcatca agcagaacat
     3601 cgatgtgctc gtcgcaaaat tcgggattga gtacagcaag gggcaggaaa tcactgctgc
     3661 gctctttcct aatcctgtag ccacaatcgg aacgctcagc tcgttcacgc aagggcggac
     3721 tgcatcgaat agcggggcgc tgttcggtca ggtccagcag ttattcgaat tggcaggaaa
     3781 acgcggctat cgaattgaga gttcggccta tggcacccaa tcgtcggaag cggcgtttga
     3841 agatgcggtg cgccaattag gttttactgt caaggacacg tattaccgga tccagttggc
     3901 tcagcggcgt ctgacgctgg cagaagaaaa tcgtgatcgc tttgcccgga tcctcgatat
     3961 caatacgata cggtttaaga aggggtatat tgcagaagtc gacctcatca gaatccggtt
     4021 gcagatggtc gattttcaat cgcaggtcat tcaatcgttg caagaggctg aaacggctcg
     4081 aggcgatctg cgacaactct tgcggctgtc gccgaaaatc acgctggagt tgacgacgga
     4141 cctcgatttc cgacgcatcg atccggacat cagcaagctg cgtaccgtcg cgctcgatgt
     4201 tcgtcccgat atcaaggcgc gacgatttac ctactcgcaa cgtgagtccg atctcaagct
     4261 cgcaaaggcc tatcgcgtcc cagatctcac ggtcggaggc gggtatacca ttcagggagc
     4321 ccaagggccc gacaacccgc aacaatgggc actcaacttg ggactgccat taccgttatt
     4381 caatcgaaat cagggcggga ttcgccaggc cgaggttgct gtccagacgg ccgaagccga
     4441 cttgaataag accctcaatc tagttgagaa tcaagtggaa gttgcctatc gcaacttact
     4501 gcaaagccgt cggcttgtgg aagcgtacgt agggggggtc ttagaagatg cccgtgcgac
     4561 tttcacgatt gtcgaacggg cctatgagcg aggaggggca accattctcg acctccttga
     4621 cgccgcgcgt acctcacgga cgatccagca aaattatatt gaggctcttt tcaactacca
     4681 gcgcaatctg cttcagctcg aatccgcagt aggacaggag atcgcttcat gatgcgaggg
     4741 accccatcag cctggatcac gatcctactg attgcttctg taggttgtgg aaagggagat
     4801 cagccgggaa attcagatgc ggcgacgcct cctgcgaagc gtgcggtgat ttccaccgat
     4861 ataggttccc gcgtcgaaac agggctggtt gattacagtg gatcgcaaca ggacctgagc
     4921 ttggccggaa aagttgccta cggcgaagat cggtactcca agatttcctc gccgttgcaa
     4981 gggcgcgtgg tcgaagtccg ggttcacttg ggcgatcgcg tcaaggccgg agatgtgctg
     5041 atggtggtcg atagtcccga tatcgcgcaa gcatactccg agtacgtcaa ggaggattcc
     5101 gatcttcagt atgccaccag ggcgcacgaa ctggctaagg acctctatga aaacaaggcg
     5161 atgcctctca aagacttgaa gcaggctgag aacgagttgg taaaggcccg cgcggagttc
     5221 cggcgcgcga aggagcgact gctctctttg cgcattccag ccgaagagtt gagcaagcct
     5281 ctggacaaac agaagatcac gtcccgattt gagatgaaga gtcccctcac gggcatcgtc
     5341 gtcgagcgtt cagtcacgcc gggccagtct gtaagcggag attcggacca tgtcctgttc
     5401 accgtcgccg acttggacat gctgcaggtg gtcgcagacg tgtacgagcg tgatctggcg
     5461 ttggtgaagg aaggccagtt tgccatggtc aaagtcgaag cttatccgga tgtcgatttt
     5521 cccgcaacgg tggcgtccat cggcgatgtg gtggatccgg ccagccgcac catcaaattg
     5581 cgcgcatggg tcaataatca agaccacaga ctgaagcccg aaatgtttgc gcggttgcat
     5641 atccaaatcg gcgatgccac gcgaattctt gtcgtaccga aggaagcggt cttggaagtc
     5701 gacggccggc agttcgtcta catcgttgaa gatagcaatc gctatgtaaa gcgagaggtc
     5761 aaagccacaa acttttcccc ggatcagatg cgtgttctcg aggggttggt ccccggccaa
     5821 cgcatcgtga tcaaaggcgc ggtgctgatt aagggccaag aagtcaaagg tggctgaggc
     5881 ggtgtgctcc cttcctcggt aatccctggg taaattgact cgatgatcgc acgcgtcgta
     5941 gagatatccc ttgtccagcg gattatggtt tgtgcgctcg gatttgcatt gttgttcggc
     6001 ggactttacg cgtttcatct cctcgacata gtggcctacc ctgacccttc tcctccgatg
     6061 gtcgaactga ttacccaaaa tcctgggtgg tcgtcggagg aaatggaacg tcagatcacg
     6121 attcctctcg aagtcgcgct gaccggcatg ccaggcctga cggacattcg gtctctgtcg
     6181 atcttcggcc tgagcgacat caaagtctac tttgatttcg ggacggatat tttccgagat
     6241 cgccaagagg tgttgaaccg aattgcatcc gtcgagctgc cacaaggtgt gcaaccgcag
     6301 ctgtctccct ggtgggcgat cgcggagatc tatcgctacg aattgaccgg tgatcccggc
     6361 gtcagtttga ccgatctcaa gaccattcag gattggcagg ttcgccgctc tttccgccgc
     6421 attccggggg tcatcgacgt gacaggattt ggagggacca ccaaggaata tcacgtcgac
     6481 atcgatcctg ggaaattgat cagttatgga gtcaacctct cgcaggtcat gaccgcgctc
     6541 cagaagagta atgcgaatgt cggcggcaat tacctcgcac tcggcgcgca gaactacaac
     6601 atacgcggct tgggtctcat caacagtatc caggatattg aaaatgtgat ggtcgtgcaa
     6661 aaagagggga ctccgatttt tgtcaagacc ctcggtcagg taaaagtcgg aaatcgtgtg
     6721 cgtctcggca aagtcgggat cgatgaccgg gatgatgtcg tggaaggtgt cgttctgctg
     6781 caacgggggt ataaagccct cccggtgctc gataaggtgc gacagaaagt cgatgatctc
     6841 aacacgtgga agttaccccc gggagtcaag gtcagaacat tttatgatcg gactgcgctg
     6901 atcaacacga cggttgaaac cgtcacggac attctcatca gcgggatggt gctggtgttt
     6961 gtcctgctct atgtgtttct cggcaacatt cgtgcggctg gcatcgttgc cctgacgatc
     7021 ccgttgtcgc ttctatttac cttctcaatg atggtggtaa tcggtcaatc ggctaatctg
     7081 atttcactgg gctcgatcga ttttggcatc atcgtcgatg cgacgctgat catcgtcgaa
     7141 agcatcttct tcaacctctc ccatgggaag acgccgggtt tgacggttca tcagcagatt
     7201 gtgcgggcag ctcggcaggt cgggcagccc attttttatt ccaccacgat tatcgtggtc
     7261 gcatttatcc cgttgtttac gatgaccggc gtgcccggga aaatatttgc ccccatgtcc
     7321 atcacctatg ggtttgcctt gctcggcgcc ctgttgatgg cgttcacctt ggccccggtc
     7381 ctctgctcat tcctcctcaa agggcatatt aaggaggaag atacagcggt ggtaaggccc
     7441 ctgcgtcgtc tgtatcacgg gacattggaa tgggcgcttg accaccgcaa aagcgtcgtg
     7501 atgtttgcct gtatgttact cgtcgtggca ctcatcgcct tgcagttcat gggtggcgag
     7561 ttcatgccag ccttggaaga ggggaatctc tgggttcgtg caaccatgcc cgtcgatatt
     7621 tcattcgacc aggctgcgcg tcttacgacc gatatccgtg cgatgtttcg agagtcgccg
     7681 gaggtgacca cgatcgtgtc gcagcttgga cgccctgatg atggcaccga tcccaccagc
     7741 tttttcaacg cagaattcct ggcgaatttg aagccgcagg cggaatggcg caaggggttg
     7801 acgaaagatg cgctcattga agaaatcgaa gggaagttgc ggacgattcc gggcgtcatt
     7861 ttcaactttt cgcaagtcat ccaagacaat gtggaagaag cgatgtcggg tgtcaaaggg
     7921 gagaactcca tcaagctatt cggaaccgat ttaaaagtta tggaggctgt ggcggtcgaa
     7981 attgagaagg agatgagcca gattcgaggg gtgaaggatt tggggatttt ccgattggtg
     8041 gggcagccga atttattgat caaagtggat cgggaagcga gtgcgcgcta tggacttcaa
     8101 gtatcggacg taaatgccgt ggtgcaggct gcggttggcg gccaggcggt gacgcaggtg
     8161 tatgaagggg aacggctttt tgatctcgtc gtgcgattcc tgcccgagta tcggcaggat
     8221 atggaatcga tcagcaatat tctcgtgagc acaccggacg gagcgcgcat cccgctcaag
     8281 caactcgcga cgatcacgac ccagacgggg gcgttcatca tctatcgtga aaataacgaa
     8341 cgatatattc cgatcaaatt cagcgtgcgt gatcgggatc tccagagcac cgtcgaggaa
     8401 gcacaggcgg tccttgccaa gaaggtgaaa cttcccgaac ggtatcgtat ggagtgggcc
     8461 ggtcaatacg accaactgaa ggatgagcag aatcgattgg cgaaggtggt tcccgtgagt
     8521 ctccttctga ttctctttct tctctacacc actttcgact cactgaagaa cgccttgctg
     8581 gttctctcag ccgtgccgtt tgccttgatc ggcgggatcg tcgcgctggt tgcgacgcat
     8641 acgcatttca gtatttccgc cgcggtcgga gtgatttcga cgttgggagt tgccattctt
     8701 ggcggtgtct tactgatctc acggatcgaa gattttcgac agacggggtt ggatcttcgt
     8761 gaagccgttg tcaaagcggc ggatgtgcag atgcggccca ttctcatggc cacgctcggc
     8821 gcggccattg ggttgttgcc tgccgctttg gccacgggga tcggatccca ggcgcagaag
     8881 ccgttggcac gggtggtggt cggaggaatg ctgacggcgg ccttcttgat tcttgtggtt
     8941 ttgccggtgt tatatgaaat ggtgcaccgg cgggcaggca aacactaacg aaagaggatc
     9001 atggtgcgtt acgcatatgg atggatggtt gtggcagtcg tggcggttgt gaccgcgggc
     9061 gcgggctcgg cacgtgccga ttccttgggg acagggcggg tatttccggt tcagatgccc
     9121 tatgaaatgc cgccgaaagg agaggatacg ccggcggtgt ccgttccgca gaataccaaa
     9181 ccgttaagcc cggaagaact gcagcgggct gaagcgctgc ttccgttatt ggaaggcaaa
     9241 caggaatttt gggcgatggg ggaattcgtt catcttggtg aaccgagcgt ccccgtcctc
     9301 gtgaaggcgc tcaccatgcc agggccgagg attcggtata atgcgattga gacgatctcg
     9361 atgttgcacg cggtgtcggc agtgccggcc ctcattacga cggccaagga gcccaacgag
     9421 attccacgtg tgcgggagca tgcgcttcgg gttgccatcc ggctagatcc ctccaagtcc
     9481 cccgaggcga ttcaagtgat gtcgaaagat cccaactcct cgattcgaaa ggccgccgct
     9541 ttcgaggcgc ggtatgtccg ggagaagacg gtgattccga ttctgatcga catgatcgat
     9601 gatgacgagc ggtttgtggc cctgtccgcg gtgcagtcac tatggattct gacgctccat
     9661 gagacggaat tccatgactg ggatgcctct tccaagcaag atcggcagga atggatggcc
     9721 gaatggacag aatggtggaa cgggcagaag gacacgtttc agattccgga acccaagcgc
     9781 cgctcacaag ggcgtcaggg ttaagccgat cccttgcggg tcaaaaatgc cccgtgttat
     9841 aggtgtaagc cgatcggcaa acccttcatc aaccgttatg ttaggctagc aatcgtttat
     9901 ggctgtcctt cctatcgcaa aactcggcaa tcctgtgctg cgcaaaatct cgagcgcggt
     9961 cgatcctcgc gacattcgca caagggaatt tcagcgcttc atcgacgaca tgttcgaaac
    10021 gatgttagat gaaccgggga tcggcttggc ggcgccgcag gtgtcccgat cgattcaatt
    10081 gattgtgatg ggatgcgagg gagagggtgg tttcccgacc accgtcctga tcaatcccaa
    10141 gatcgtctat tacgggccgc aacaggccga aaattgggaa ggctgcctga gtgtcgatgg
    10201 tctgagaggg aaagtgacga ggccatcggt ggttcgcgtg caggcgctcg atcgcgacag
    10261 caagcctgtc gatatcgagg cagacggcct ctacgccgtg tgcattcaac atgaaatgga
    10321 tcatctgatt ggaaaactgt tcttggaccg catggcggac ctgtccaccc tgacccagtt
    10381 aaccgaattc gacaagtatt ggcggaaaga gccgaccccc gtcatttagc ttaccggttc
    10441 agtcaatcat cgtgaaaact ctacttcggg tcctgcaata tctccgcccg caccgtgcga
    10501 tggtcgtcgg caccttcgtg ttcgcaggcc tgacgacggc gtttgagctt gtgccgccct
    10561 ggctcatcaa gattgtgatc gatgacgtga ttcaggccgg caagacccaa ctgctcgcgg
    10621 ttgtatttct gggtctctgc ggcgcgtacg tcctgcgcaa cgtgtgcgca tcgacgcgca
    10681 tcaagctgaa caacagcctg gagcagcaga tcgtccacga cttgcacgtg caggtgttcg
    10741 gcgcactcca gcgtctctcg atcagtttct ttgagaatcg cccgaccgga gaagtcatgt
    10801 cgcgcgtgct gaacgacacc gaacatatgc agcgcatctt tgtcgacggc ctcgaagaga
    10861 tcattacggc cgggctgacg ctggtcggca ttatgaccgt tctgttttgg cttaattgga
    10921 agctggcgct gttagccctg gtcccgattc ccatcctcgt agtgggggct gtggcgttta
    10981 ccgggaggat tcacgggcat tatcgaaaga tacggaaggg gtccgcggaa ctcaatgcgc
    11041 tcttgcagga ttccctggcc ggcatccgag agacgatggg gttcaaccgc cagccttatg
    11101 aagaggatcg ttttggccgg aagagcgatc aatgccgccg cgatacgctg aaagcgatgt
    11161 atctctggtc ctactattca ccggggatga tgctgatcgg aagtctcggt gccgcgctcg
    11221 tcctgtggca cgggacccag gaagtgctgc agcagcatct ctccgtcggt gaattggtca
    11281 tgttcgtctc ctatctcggc ctcttctacg tgccgatcaa ccagatccat tccgtcaacc
    11341 acatgctcca gcatgcgctg gctgccagtg agcgggtgtt tgaagttctc gacattgtgc
    11401 cggatgtcaa agatcagcct ggggtgcggc ctcctgtgcc tcggctcagg ggggagatcg
    11461 aatttcaccg cgcgggattt cattatcgta atgaggcgcc gatcttgcgg gacgtgtccc
    11521 tgttcgtgaa agcgggggaa cgcgtggcgc tcgtcggccc cagcggggct ggaaagagca
    11581 cgacgcttaa actcatgaca cgtttttacg atgtgacgga gggcgcgatt accattgacg
    11641 ggtacgatat tcggcagatg ccgctggcgt ttctcaggag tcaaatcggg ttagtacagc
    11701 aggagccgtt cctgttcaat ggcacggtgc gagagaatat cctgtacgga aatctttccg
    11761 ccagccaggc tgaggtcgag gccgcggcaa aggctgcccg ggcccatgag tttattgtga
    11821 cgcttccaga gggatacgat acatggattg gcgagcgggg cgtgaagctg tctgtcggcc
    11881 agcgccaacg cgtgtcgatt gctcgcgtgc tgctcaagga tcctccaatc gtcatgttcg
    11941 acgaagcgac atcgaacatt gacacggaaa ccgaggtcaa aatacgagag gccctggacg
    12001 agttgacaag agggcggacg acgatc
//
LOCUS       DATIFR010000078         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1199090, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000078 DATIFR010000000
VERSION     DATIFR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199090"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3013
                     /locus_tag="VLD60_04225"
     CDS             <1..3013
                     /locus_tag="VLD60_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSB44193.1"
                     /translation="AATTIKSAKRLVEKERPEVWDVLDEVIREHPVLLNRAPTLHRLG
                     IQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAVHVPLSVEAQVEARVLMMSINNIL
                     SPANGKPIAVPSQDMVLGCYWLTKERVGVKGEGKVFGSPEEVRIAFDAREVEEHARIK
                     VRIEGNLVQTTVGRVILSEVLPPGMPFAYANKLMTKKEVTKLIDSVYRQSGHRETVTF
                     LDKIKDTGFYYATRAGVSICIDNMHIPSKKEELIGKAQREVNEIERQYAEGLITNGER
                     YNKVIDIWAHVTEQVAGEMMKELGAGGDPAKDEAFNPIFMMADSGARGSSQQIRQLGG
                     MRGLMAKPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRL
                     VDIAQDVIISEIDCGTSDGIFVSALVEGGEIIQPIEERILGRLAAEDIRDPVTGEVIV
                     SFNEEITEERTKDVVEAAVDKVKIRSVLTCQSRRGVCRACYGRDLARGRLVEKGEPVG
                     VIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLEAKHAGRMKFMSFDAKKNADVHNG
                     GIAVRNKEGEWVVMNRNAKIAIVDESGREREKYPVVYGAKIKIKDGDRVELAQKLVEW
                     DPYSLTILTEVGGKVAFGDIVEGVTMKEEFDEVTGLSRKVIIEHSGATLRPRVSIKDE
                     SGKTAKVSGGVNTVARYLLPVGAHIFVEKGAAVHPGDVLAKIPRETTKTKDITGGLPR
                     VAELFEARKPKEQAVISEIDGEVSYGGFVKGQRKVLVDNKMGDVKEYFIPKGKHVNVH
                     EGDWVRAGEPLMDGSANPHDILDVLGPNELQKYLVDEVQDVYRLQGVTINDKHIEIIV
                     RQMLRKVRIEDPGDTEFLPGSQVSKMLFEEENDRVLKKGGKPALGKPVLLGITKAALT
                     TDSFISAASFQETTRVLTEAAINGREDNLLGLKENVIVGRLIPAGSGFEEYRDTFVIS
                     PKPEPVVVGQADASSLPREGATAASGDGGGA"
     gene            3134..3505
                     /gene="rpsL"
                     /locus_tag="VLD60_04230"
     CDS             3134..3505
                     /gene="rpsL"
                     /locus_tag="VLD60_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSB44194.1"
                     /translation="MPTINQLVRKGRQLAHAKTKSPALKSCPQKRGVCLRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAVGVADRKQGRSKYGAKRPK"
     gene            3542..4012
                     /gene="rpsG"
                     /locus_tag="VLD60_04235"
     CDS             3542..4012
                     /gene="rpsG"
                     /locus_tag="VLD60_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSB44195.1"
                     /translation="MPRSRFLGQREPLPDVRYRDKVVGKFLNILMEGGKKSTAERICY
                     GAFDVIQEKTGGDPLKVFKAAIDNVKPVVEVKSRRVGGASYQVPVEIRPARRMSLALR
                     WLSEFSRTRGGKSMREKLAAELLDASNNTGAAVKKREDVHRMAEANKAFAHYRW"
     gene            4126..>4584
                     /locus_tag="VLD60_04240"
     CDS             4126..>4584
                     /locus_tag="VLD60_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB44196.1"
                     /translation="MARQTSLDHTRNIGIMAHIDAGKTTTTERILYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFDSVQGVEPQSETVWRQADKYSVPRIAFMNKMDRIGADFYVSVQTMIN"
BASE COUNT         1077 a         1143 c         1468 g          896 t
ORIGIN      
        1 cgcggcgacg accatcaaga gcgccaagcg tctggtcgaa aaggagcgtc ctgaagtctg
       61 ggacgtattg gacgaagtca ttcgtgaaca tccggtgctg ttgaatcgtg ccccgacctt
      121 gcaccgattg ggcattcaag cgttcgatcc tgtgttggtg gaaggaaaag ccatcaggct
      181 gcatccgctg gtctgcgcgg cctttaacgc cgacttcgac ggagaccaaa tggcggtgca
      241 cgtgccgttg tcggttgaag cgcaagtgga agcgcgcgtc ctgatgatgt cgatcaacaa
      301 cattctttct ccggccaacg ggaagccgat tgctgttcca tcacaggaca tggtccttgg
      361 ctgttactgg ctcaccaaag aacgagtcgg tgtgaagggc gagggaaagg tgttcgggtc
      421 tcccgaggag gtgcgcattg cattcgatgc gcgtgaagtc gaggagcacg cccggatcaa
      481 ggtccggatc gagggcaacc tggtgcagac caccgtcggt cgcgtcattc tttccgaggt
      541 cctgcctccc gggatgccgt ttgcctatgc gaacaagttg atgacgaaaa aggaagtcac
      601 gaaactcatc gactccgtct accgtcagtc cggccatcgg gagacggtca cgttcctgga
      661 taagatcaag gataccggtt tctactatgc gacccgcgcg ggcgtgtcga tctgtatcga
      721 taacatgcac attccgagca agaaggaaga attgatcgga aaagctcagc gggaggtcaa
      781 tgaaatcgag cggcagtatg ccgaagggtt gatcaccaac ggcgagcggt acaacaaggt
      841 gatcgatatt tgggcccacg tcaccgagca ggtggcgggc gaaatgatga aggagttggg
      901 cgcaggcgga gatccggcca aggatgaggc gttcaacccg atcttcatga tggccgattc
      961 aggagcgcga ggcagctctc agcagattcg gcagcttggc ggcatgcgcg gactgatggc
     1021 caaaccgtcg ggagaaatca tcgagacgcc cattacggcg aatttccgcg aaggtctcac
     1081 cgtgttgcag tacttcatct cgacacacgg agcccgcaaa ggattggccg acaccgcgtt
     1141 gaagaccgcg aactccggat atctcacgcg acgtctcgtc gatatcgcgc aagatgtgat
     1201 cattagcgag atcgattgcg gcacgtccga tggaattttc gtgagcgcgt tggtcgaagg
     1261 cggagaaatc attcagccga tcgaggaacg tatcctgggc cgcttggcgg cggaagacat
     1321 acgcgatccc gtgacgggag aagtgattgt ctcgttcaat gaggaaatta ctgaagagcg
     1381 gacgaaggac gtcgtggaag ccgcagtcga caaggtcaag atccggtccg tgttgacctg
     1441 tcagtcccgt cgcggggtgt gccgggcctg ctacggccgc gatcttgcca gaggccgctt
     1501 ggttgagaag ggcgaaccgg ttggggtcat cgccgcacaa tccattggtg agcctggcac
     1561 gcagctgacc atgcggacct tccacatcgg tggtacggcg agcaaggtgg tcgagcaaac
     1621 ggtactcgaa gcgaagcatg cgggtcggat gaagttcatg agtttcgatg ccaagaagaa
     1681 tgccgacgtg cacaacgggg gcatcgccgt tcgcaataaa gaaggcgaat gggtcgtcat
     1741 gaacaggaat gcgaagattg cgatcgtgga tgagagcgga cgcgagcggg agaagtatcc
     1801 cgtcgtctat ggtgccaaga tcaagatcaa ggatggtgat cgggtcgagt tggcacagaa
     1861 gcttgtggaa tgggatccct attctttgac gattctgacc gaagtcggtg ggaaagtggc
     1921 tttcggcgac attgtcgaag gtgtgacgat gaaagaagag ttcgacgagg tgacggggct
     1981 ttcgcgcaaa gtcatcatcg aacattccgg cgcaacattg cggccgcgcg tgtcgatcaa
     2041 ggacgagagc gggaagactg ccaaagtttc gggcggcgtg aataccgtgg cgcgctattt
     2101 gttgccggtc ggcgcgcata tcttcgttga gaagggcgct gctgtccatc cgggcgacgt
     2161 gctggcaaaa atccccagag aaacgacgaa gaccaaggat atcaccggtg gtctgcctcg
     2221 cgtggcggaa ttgttcgagg ccagaaaacc caaagagcag gccgtcatta gtgagatcga
     2281 cggtgaagtg tcctacggcg ggttcgtcaa gggtcagcgc aaagtgctcg tcgataacaa
     2341 gatgggcgat gtgaaggaat acttcattcc caaaggcaag cacgtcaacg tgcacgaagg
     2401 tgactgggtg cgagccggag agccgctgat ggatggctcc gccaatccgc acgacatcct
     2461 cgacgtgctc ggcccgaatg agctgcagaa gtatctcgtc gacgaagtgc aggatgtcta
     2521 tcgcctgcaa ggtgtgacga tcaacgacaa gcatatcgag atcatcgtgc gccagatgtt
     2581 gcgaaaggtg cgcatcgaag atccgggcga cacagaattt ctgcctggaa gccaggtcag
     2641 caagatgttg ttcgaagaag aaaatgatcg cgtcttgaag aagggcggaa agcccgcgct
     2701 cgggaagccc gtactcttgg gcatcacgaa agccgcgttg accaccgaca gcttcatttc
     2761 ggcggcgtct ttccaggaaa ccacgagagt cttgacggaa gcggcgatca atggacgtga
     2821 agacaatctc ttgggtctga aggaaaacgt cattgtcggc cgactgattc cggctggcag
     2881 cggattcgag gagtatcgcg atacattcgt gattagtccg aaacccgaac cggttgtcgt
     2941 aggccaagcg gatgcttcaa gcttaccgcg agagggagct accgccgcat caggcgatgg
     3001 cggaggcgca taaacattga tgattcgtgc ctcgcttgac agcgagacac ttgatcatta
     3061 taatccgcgg gctttgcact tgcatgggtg cgagcgggga ttaaactgtt ctgaagacga
     3121 gagaatacga ccgatgccaa cgatcaatca attagtgagg aagggccgcc agttggccca
     3181 cgcgaagacc aagagccctg cgctgaagtc ctgcccgcaa aagcggggag tctgcctgcg
     3241 cgtgtatacg tccacaccga aaaagccgaa ctcggcgctc cggaaagtcg cccgcgtgcg
     3301 gttgacaaac gggatggagg tcaccactta catccccggt gtcggacata atttgcaaga
     3361 acactcgatc gtccttgtgc gaggcggtcg cgtcaaagac ttgcccggcg tgcgctatca
     3421 catcgtccgc ggtgcgctcg atgcggtcgg cgtggcggat cgtaaacagg gccgatcgaa
     3481 gtatggcgcg aagcgcccga aataaaaact tcataccgaa gctttaagag agtacacgac
     3541 aatgccacga agcagatttt taggtcagcg agagccgctt ccggacgtgc gttaccgcga
     3601 taaggtcgtg gggaaatttc tgaacatttt gatggaaggc ggcaagaaga gtacggcaga
     3661 gcgaatatgc tacggcgcct tcgacgtcat tcaggagaag accgggggag atccgttgaa
     3721 ggtgttcaag gctgcgatcg acaacgtcaa gccggttgta gaagtcaagt ctcgccgggt
     3781 tggtggcgcg tcctatcagg tgccggttga aatccgtccg gcacgacgga tgtcgctcgc
     3841 gcttcgttgg ttgtctgaat tttcgcgcac gcgtggcggc aagagcatga gagagaagct
     3901 cgcagccgag ctgctggacg cgtcgaataa tacaggggcc gcggtgaaga aacgggaaga
     3961 cgtgcatcgg atggcggagg caaacaaagc cttcgcgcac tatcgctggt aacgtctcgt
     4021 tctgtgctgc agttgggccg tgctgcgatc cgcagacgcg gagtgcccct gaatcgcagc
     4081 gattatcggc tcattctgca gcacgtgtat ttttaggggt tgtacgtggc taggcaaaca
     4141 tcattagatc acactcggaa tatcggcatc atggctcaca ttgatgccgg caagacgacg
     4201 accactgagc ggattctcta ttacacggga atgacgcata agatgggtga ggtccacgag
     4261 ggcgcggcca cgatggactg gatggagcag gagcgggagc gcggcattac gatcaccgcc
     4321 gcggcgacca cctgtttttg gcgggatcac cgcatcaaca tcatcgacac gcccggtcac
     4381 gtcgatttca cgatcgaggt agaacgatcg ctgcgcgtgc ttgacggggc tgtggcggtg
     4441 ttcgattccg ttcaaggtgt tgagccgcaa tctgagacgg tgtggcggca ggcggataag
     4501 tatagtgtgc cgaggatcgc gttcatgaac aagatggacc gaatcggggc ggatttttat
     4561 gtcagcgtcc agacgatgat caat
//
LOCUS       DATIFR010000079        34142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2731418, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000079 DATIFR010000000
VERSION     DATIFR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34142
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731418"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..808
                     /gene="purC"
                     /locus_tag="VLD60_04245"
     CDS             104..808
                     /gene="purC"
                     /locus_tag="VLD60_04245"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSB44197.1"
                     /translation="MTTGSMLYEGKAKKIFATDKPDQVVHYFKDDATAFNAQKRGTIV
                     EKGIINNKVSERLFRLLEQNGVPTHFVQRLSDREMLTKKVTIVPVEVVVRNVVAGSLA
                     KRLGLKEGDPIQPAIVEWYYKNDALGDPLIADEHVRLLKLATPEQMGEIRRMALKVNG
                     LLQPFFAERKMILVDFKLEFGLHNGQLILADEISPDTCRFWDQTTKESMDKDRFRKDL
                     GKIEEAYQEVLKRVCG"
     gene            1063..1278
                     /locus_tag="VLD60_04250"
     CDS             1063..1278
                     /locus_tag="VLD60_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44198.1"
                     /translation="MLRAFLNYGLVAAVVVWAAVVGMMAYRLEESPWRWAFIALVFAG
                     GLTVAGIFWIRRYVNALNKASEEQGKP"
     gene            1547..1789
                     /gene="purS"
                     /locus_tag="VLD60_04255"
     CDS             1547..1789
                     /gene="purS"
                     /locus_tag="VLD60_04255"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HSB44199.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEV
                     DVNETDKAKADAQVKSMCEKLLANTIIEEYRYELSA"
     gene            1830..2552
                     /gene="purQ"
                     /locus_tag="VLD60_04260"
     CDS             1830..2552
                     /gene="purQ"
                     /locus_tag="VLD60_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSB44200.1"
                     /translation="MKIGVVVFPGSNCDHDCQHVFRDVLGQSVEMIWHKETFLAGLDA
                     IVVPGGFSYGDYLRTGAIARFSPVMGAVKEFAKKGGLVIGICNGFQILLEAGLLPGAM
                     LRNRSLHFICKDVYVRVENAATPFTSACKPGQVLKIPIAHAEGNYYTDPVTLAGLQAN
                     AQVVFRYCTADGEVTPEANPNGSLDNIAGIRNAEGNVLGMMPHPERCAEDMLGNEDGR
                     IIFLSMIDSLKKATSPLSGVGS"
     gene            2549..4804
                     /gene="purL"
                     /locus_tag="VLD60_04265"
     CDS             2549..4804
                     /gene="purL"
                     /locus_tag="VLD60_04265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HSB44201.1"
                     /translation="MKATNPTIITKDLIAQHNLTDDEYKKIVEILGREPNLTELGMFS
                     VMWSEHCSYKSSRVHLRKLPTTGPRVVQGPGENAGAVDIGDGLCVVFKMESHNHPSFI
                     EPYQGAATGVGGILRDIFTMGARPIALLDSLRFGQLDTAKNRHLMKGVVAGIAGYGNC
                     MGVPTVGGEVRFDDIYSLNPLVNAFCLGVAHKDKIFRGTAAGVGNPVIYFGSKTGRDG
                     IHGATMASDSFDDQSEQKRPTVQVGDPFQEKLLLEACLELMAGDLLVGIQDMGAAGLT
                     SSSCEMASRAGNGIELDLTDVPRREPGMTPYELMLSESQERMLMVAKAGKEEECIRIC
                     KKWDLDVAVVGKVTGDGLLRVIDQGKVVAEIPAKSLADDGPRYERPYSPPAYHDMLTN
                     LNYDAIPDVKAPNAALLSLLESPTIASKRWVYEQYDHMVRTNTMVRPGSDAAVMRIKG
                     TNKAVAMTVDCNGRYCLLHPYEGARLAVVEAARNLVCSGAEPIGLTDCLNFGNPERPD
                     IMWQFVLAIEGMKDACEHFKIPIVSGNVSFYNETNGLSIYPTPMLGMVGLIEQVDRAM
                     TQWFKDEGDVILLLGKTRDDLGGSEYLRVLHHREQGSPPFLSLETEKAVQDFILKVIQ
                     EGLVQSAHDCSDGGLAVALAECCVSAPDVKRGAVIRLTLESLRRDALLFGESQSRVVL
                     SVKPEHAESILSRAWDGGIPAMKIGTAGGERFVIDVEKGQWSDGCRLDLPIDQLYDRW
                     AFSIERTLNQA"
     gene            4832..6304
                     /gene="purF"
                     /locus_tag="VLD60_04270"
     CDS             4832..6304
                     /gene="purF"
                     /locus_tag="VLD60_04270"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSB44202.1"
                     /translation="MSQNQGFLPHASRLTPHTLPMISPDKFHDECAVFGIFGHKEAAN
                     LTYLGLYALQHRGQEASGIVSGDGEQFHAHKGMGLVADIFNDSILKNLVGTTAIGHNR
                     YSTAGGQDLKNVQPLTVNFAFGNLALAHNGNLINAQVLRNELEAYGAIFQSTSDSEVI
                     IHLIAHSRADTLLARIIDALSQVRGAFSVVLLTDDGIIAARDPHGFRPLCLGRLRDSY
                     LVASETCAFDLLDAELIREIEPGELVVLNEQGVTSYKPFAPVQPAMCVFEYVYFARPD
                     SKIFGANAVYATRKALGRQLAQESWVPADIVIPVPDSGVPAALGYAEGGGIPFETGLI
                     RNHYVGRTFIEPEQSIRHFGVKVKLNAVPEVLHGKRVVVVDDSLVRGTTSRKIVKMIR
                     QAGAKEVHVRISSPPIISPCFYGIDTPTKKELIASSHSTEEIRKYITADSLAYLSLEG
                     MLKSAPGTPGQYCNACFTERYPISFTRAEEFQLGLFERST"
     gene            6568..6948
                     /locus_tag="VLD60_04275"
     CDS             6568..6948
                     /locus_tag="VLD60_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HSB44203.1"
                     /translation="MRQPFSTPDHEAEVAAMTIQREELSQGEDWGRLRELVPVIHPKV
                     QGVALEGETVLFDLRTGRSYRLNRVGTAIWEQCSGTATLEEIHRSVCAHLNLSLERVH
                     DHVLSFVAQWSHEGLLTHGFSAKQ"
     gene            6997..8022
                     /locus_tag="VLD60_04280"
     CDS             6997..8022
                     /locus_tag="VLD60_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44204.1"
                     /translation="MTIAVRPLEYRFSFHGVGLRYLTSSPTLRASMAEWLKHFRGDGP
                     TEGVSLTIHFEEAPGRDAVPLALSPAARRLFSGTKPVMGSAMRALWQCDILQDGSHLV
                     VDIHDQGLLDIDGAQGTARGYFYHPAAMHQDRLEGFFHYALAELLKRNNMFTLHATAL
                     EYHGRGLLILGNTGCGKTTTLLSLLRSGYRYLSDDHPVLWDSGTRLELRAAPMKIDVT
                     EQTIGFFPELRDAMSGLLRQGAYKKSFQLEDIYSDSAGRSCEPTMILFPHVTHMPHSC
                     LEPLPKSRALELFMPEQSLVYDEEAAAREFQALSKLVQQAACYRLHFGQDVLDLPQLI
                     TPLLERH"
     gene            8222..8755
                     /locus_tag="VLD60_04285"
     CDS             8222..8755
                     /locus_tag="VLD60_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB44205.1"
                     /translation="MKRLALFLSVLIFPVFAAHLLLAAGFFKVGEKAPSFSLNAITGE
                     AVSLDAYKGKVIVLGLFHICDPCMMQGSNLQKVHEAMQGKNVAVIGVNSNGNSKKDVG
                     EFLSAFPVKVTYPYLLDPGKVTDKLYGGGKFIPNVYVIDQNGIIRWQRVGNMDLAGAD
                     VIVAEVEKLLAGGSSKM"
     gene            8900..9139
                     /locus_tag="VLD60_04290"
     CDS             8900..9139
                     /locus_tag="VLD60_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44206.1"
                     /translation="MEELEEGKQKFLEVVKAIDEGVHVVIPVTPSNSMFLISLTKGSN
                     RKFITVPEDDIIDLPNEAGILAKVTKTVKDAVASL"
     gene            9143..9739
                     /locus_tag="VLD60_04295"
     CDS             9143..9739
                     /locus_tag="VLD60_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44207.1"
                     /translation="MMKQVLPGFWQWSWFSEEKQLDFNGLFLVVGEHKILIDPPPIAG
                     EDQSFIRRNGPVDYLIVTNRDHAREAGPCQGNFGCQLFVPEADASQMDLKPTKTFKDQ
                     ELLPGGLWVVHLKDQKSPGESALFIQLGKGILIVGDALIGKPAGALSMLPAEKYADAA
                     KAKEGLRRLLKYNFDAMLVGDGTSILTGAKQAVERALQ"
     gene            9752..10576
                     /locus_tag="VLD60_04300"
     CDS             9752..10576
                     /locus_tag="VLD60_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44208.1"
                     /translation="MTLRNGLSEELNRQGNEHFGRGHYTDAYTCYAKALECDRITGDQ
                     RALAATLGNLGNICAVSGRREAAQNYYHEVLELQKILGDEKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDLMAKSHDEAAKAVLFSDLGLVARETGKFDDAITYYENSLVLMRR
                     LGNDGGAADAWRMMARTFLMQHRYEDAIACCQTSQSIAERARDELRAGGARYVLADCY
                     ESMGRLEEAANLLERVVDMDRKYHLPKLEENTRRLQALRDRICDASSPPTLRESHG"
     gene            10573..11010
                     /locus_tag="VLD60_04305"
     CDS             10573..11010
                     /locus_tag="VLD60_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44209.1"
                     /translation="MSEQASVLWTEVRRSLSQAFPQFYELEPGGPLALDLGNDGWLLE
                     IKPEGKLLCQYGVAMDDVMSLMSEGTPEDLGTDEVAKQAKYFIQPAVSKYRPLLLQSG
                     FSEQTDMNEEYVAVAFERTVDFENLSKVKDLIRWCCRQIGGSA"
     gene            11007..12074
                     /locus_tag="VLD60_04310"
     CDS             11007..12074
                     /locus_tag="VLD60_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB44210.1"
                     /translation="MMRAIKSFDDFRDTVSAYRLPRVILTALELKLFTVVGARIWRMS
                     DLARELKVSERGLSILCRNLAMAGLLHKKGDLYRNSRLAATALNADDPAYRGNYLNLI
                     TSHWADWMRLTESVRTGESLDKDEPEAQDYRRQFTWAMHHRTLETAPKIAAQISLKGA
                     KTLLDLGGGPGTYAMAFMANNPGLRATVCDRPAALEVAKEIAATHKAGPRLSYLPLDL
                     LKEDIPGSFDMIWYSNVLHIYSPEQNQAVFQRAKKALVPGGRLIIQDAFLHDREGLFP
                     EEASLFAVSMLLFTETGNTYSAAETARWLKEAGFGAIRMLKMKQGMEDWEGGILEAVV
                     PVGNSRTHGRQKRSTRSSRSR"
     gene            12430..12912
                     /locus_tag="VLD60_04315"
     CDS             12430..12912
                     /locus_tag="VLD60_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HSB44211.1"
                     /translation="MTDPSPDHDPRPCLLIYDGECRLCVTAKKGIEQLQSETAGSSLR
                     MITYQSEEAKQALGMRYRPGRPDVAFLVDPDGKVAKGLDAFLPLLPGLKGGRVLARVL
                     AFPLAKPLGDLLYWFVARYRYRLFGVVPLERCSEPQQSKKADSIDSHTPSSGGPTKLP
                     "
     gene            13038..13946
                     /locus_tag="VLD60_04320"
     CDS             13038..13946
                     /locus_tag="VLD60_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44212.1"
                     /translation="MRLIAWWSEVIRQLKEGFRLVQRDPVPVCSRSTGFIRPIKTYSM
                     FYVAIVGAALLGLNSANPAWSADASTEKNRKTVAAGLGYQDADISAITVKTYDAESGE
                     VLSAETYELDIKDDGPPSSRPRTRIFAGGVGVGSDGLSEFTLRVYDAANGRFLWEGRL
                     NLSSGSNPDVAAFPVVAQVQSRTAMVKVTGRTKTSGQPYFVLRAVNPETGQLAWADEF
                     SADPANVRVERISRSVIGMTGVIPREIDFRIKMPDEAGQQLLWEDKVVPAIDEEDAIP
                     ERSDDSAGKLPMWPQGSSQRVERDGI"
     gene            complement(13989..15416)
                     /locus_tag="VLD60_04325"
     CDS             complement(13989..15416)
                     /locus_tag="VLD60_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44213.1"
                     /translation="MYRRNISHVLYPAACILGLFIVYEAWLKPAYVHPPSPIQTEGES
                     QSGNETDPEPQSGEQLEPAPKQSRAIETSNVPDTKYQDDLATIRDDIEKGNLNEAETK
                     LLRLPSAVQSDALARSYVATLWNNLGIEQEKHDGTRVSVKAFKNAAALDAKNPVIQMN
                     LAHAYWEQRDPAMSQEFLERLIALAPDEPFPHLALADLLQERDRMSEAAPHLDQAADR
                     ARNDPSLQSYLRAVTAKVHRTEQAESRLNSRDGAHFTVKYDGSTDHDTWTAVLEILEE
                     AYREIGQKFGHFPSRPIVVVLHANQTFQGVTGSPAWADGLFDSILGRIQIPTQGALTD
                     RVWLKRVLRHEFVHALLQDLQGHHTTLPTWLNEGLAMQLSSDRWSDLEELNQGDVPVL
                     PLTALEGGWGGLSSEAASVAYLEANSATRYLIRRYGMHEVQQLLARMKKNQTLTSAMQ
                     SQLSLSYEQFQSRWLEQLHEEQKKG"
     gene            15563..18100
                     /gene="recG"
                     /locus_tag="VLD60_04330"
     CDS             15563..18100
                     /gene="recG"
                     /locus_tag="VLD60_04330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSB44214.1"
                     /translation="MWRSSEPKPAEQFQQWLDRLARPIEFASRDAFAHLPTVKNLSSF
                     VSTQVMQALSDRVYPRAVEAALLKLRELFGEDQQRLSQAEQQRRLQEATAILRTLRRT
                     ADDPARAWQDPEPLAWSAAVTKSAPTHELWAHPIRFAKGVGPKRMAVLQRLRIETVED
                     ALWTVPWRYEDRSVMTPIGELVPGMATSICGEVVKSEAKRTRNRRFNVLEVCVEDRTG
                     RMQVVFFNQPYLEQVLAVGKTVMMSGRVIAGRQGWMVPRMDVAQYEVVGEGTESTLHV
                     GRIVPIYHETKGWTSRQMRVLMKCLLDEHAGDLRDQLPVPVRARQRLVPIQEALQDIH
                     FPKEGAELQLLEQGKSPAHRRLAFEELFLLQVALAARQRSVQSEKKGLHFSPRTPLLE
                     KLGRLLPFQLTAAQDQVIREIFRDMISPRPMNRLVQGDVGSGKTVVALHAIVMACGSG
                     YQAALMAPTEILAEQHYRNLKGILESLGLNVVLLRGGEKAALKKVQATQLASGQIQVA
                     IGTHALIQKGVVFKNLGLAVVDEQHKFGVLQRKTLKEKGYKPDVLVLTATPIPRTLAM
                     TVYGDLDVSIIQMLPPGRKPVRTFLFGESQRRRAYQILRDELRNKKQAYVVYPLVEES
                     EKVDLQAAIQGAELLQNGEFKEYQVGLLHGRMKPAEKEAIMADFKTGKIHLLVATTII
                     EVGVDVPNATIMMIEHAERFGLAQLHQLRGRVGRAGHQSYCLLMAATMGRTRTQLGKR
                     PSGNEESSTAKERLEALVRSNDGFVIAEEDLRIRGPGEFFGFRQWGMPEFRVANIVRD
                     SDLLQQARLEAFSLLKQDPQLSAPAHQGLREAMLRKWEKKLELGSVS"
     gene            18299..18622
                     /locus_tag="VLD60_04335"
     CDS             18299..18622
                     /locus_tag="VLD60_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44215.1"
                     /translation="MGLLQRLKHDLMSGLATLRLGTAQAANRALEETELLRLRLEVRK
                     IGQQLNDLYRDVGERAVSLREAGEPAERVLYDMEIGRLVKEIHQLKEARKKLEDDMAE
                     IRNAE"
     gene            18619..19554
                     /locus_tag="VLD60_04340"
     CDS             18619..19554
                     /locus_tag="VLD60_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSB44216.1"
                     /translation="MNTQRLAGIDIGTLTCRLLIADLPSHGPLKELRSERRILRLGEG
                     VDQSKRLNAAAMDRAIHCLKEWREVIDSYRVDACTAVATSAVRDAGNRSEFLERVKRE
                     AGFDVEIITGEVEAERTLLGIRSGLPHGVTDILALDIGGGSTEFILDRPEQKPVVRSI
                     DIGVVRLCERILRHDPPTDEEIRRAREWVAGETKIAVADMGNYQTATFVGTAGTITSL
                     AAMAQKLPAYEPVRIHNYQLKLGAIHALEQELLSRKKTDRIGLPGLEEGREEVIVAGA
                     IIIRTVMETLGMSSVLVSDLGLREGVLIDLARRGR"
     gene            complement(19592..21082)
                     /locus_tag="VLD60_04345"
     CDS             complement(19592..21082)
                     /locus_tag="VLD60_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB44217.1"
                     /translation="MKPSVAVHEPRSLAGETLTLLAWHIPDLVAYQHHEDEWWFAPLD
                     DNLPIVRVNRIGLEMLKAMNGHTTVGALLEKYGTRVCGPDGQPGRWHLERWALPNFSL
                     CYFGTEPPGGHRHKAKWDVLLQQIREGWSGQEGFEGEEHLEDFHHHELTDSTQEDGHF
                     DLIETTVSHLFREPNAALNGLTYGRLLMQQLRKLGWFNPKPKILLEVGGGLGYVAREM
                     AKEILPFERQAIKYVSLDITQPFLKLQTTRAKEGGWSGIGTRANAEGLPFKDQSVDLV
                     IDNENMADMTPVKLTSKELLENKGDTEQHQEALDWIRRLRLPIEAHPPDEVIFNLGPI
                     RFLAELWRVLKPGGRAFLTEFGIEEGWPAPVKLPGHTEYEVQYSHLRQAVRWLGFQER
                     YLTLPQFLAIKPDTKVLCTGAAYTIQRFCQAMNKPFTVRAYTESELKQALGDLLPKFQ
                     GTHFHDVIDPAWFGLIDFKVVLLEKPGGAPKPTFTEQKGYRWYSQK"
     gene            complement(21176..21553)
                     /locus_tag="VLD60_04350"
     CDS             complement(21176..21553)
                     /locus_tag="VLD60_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44218.1"
                     /translation="MTLKFHLTIAVLAMGLLAVWPTTATPPHTAIIIDSGSPYFVPKL
                     ATVSTGAPIRWENPTPTEHTITHMGCMEDGEPCAFDSGIVLPNGTFTLPGLAPGRYPY
                     FCRVHPIMRGMLIVTDSFVPSQL"
     gene            21744..22769
                     /locus_tag="VLD60_04355"
     CDS             21744..22769
                     /locus_tag="VLD60_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44219.1"
                     /translation="MPEAGERRSKRGQRLCLMLLWTSLQGLLWSTTGETVWAITLTTP
                     IQGATLQSGKSVPVAVSVGKEVSLRAVRYYWYRLDEEPLASHQATAAPFVATEAGAPF
                     SGSVVVPAEALGAMRLLVVGEVARGRLESYEEFDEVLVTVETAAALSAIEFTVQKPWR
                     FDKIGKRVMVPAVGQFNDGFVRSLTGPDVGSRFRSSDDRVVSIDAAGWVQVLGSGKAS
                     IIVDHRGKSGTLEVVVEADEEPNRLPIAEVAKELQVKSGEIVVLDGLRSRDPDGDPLH
                     YEWKQVRGHRVGLTNVNESKATFVAPKVSERRMYQFSLVVTDMAGPDTIKGADSAPAV
                     ITVWVSP"
     gene            22949..23392
                     /locus_tag="VLD60_04360"
     CDS             22949..23392
                     /locus_tag="VLD60_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44220.1"
                     /translation="MAKKKRPAKKGTAKAKAKGAAKKAVKKPVAKKAAKKPSAAKAKT
                     APKKKGVEPSRSAKGAAAVTKTATAALDEEPKKATLTKPAPAMMPKEPSLDDEEIGEE
                     ELDIGGEEEIDEDIEEDLSLDDDEEDVDYLEKSEDLLDDADDYRH"
     gene            23513..23974
                     /locus_tag="VLD60_04365"
     CDS             23513..23974
                     /locus_tag="VLD60_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44221.1"
                     /translation="MSHFIFIAAALRERTSEESAELQLSLHLWGLCTALIRSNLQTYL
                     TPQSRGLVYVLKVGLCAEFHIASPPQDFSALDDFLKDELRTEARYGFVRIDGIRRWTW
                     SSQASQALLLRVLGIADQAELTRRLTLGMHRLTQAEYDAMAQGIAEGVGQG"
     gene            complement(23971..24615)
                     /locus_tag="VLD60_04370"
     CDS             complement(23971..24615)
                     /locus_tag="VLD60_04370"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSB44222.1"
                     /translation="MRQWGLAAFAFITVLVFTIPFPTLAGDLIELPVLAAVPYNNLGV
                     FEVLLLKWDRLPDPNPALLQWLQGGVRFGKTHVGAMAQAFDYAVERTPAVQHTGVVSV
                     IGVTYRPTGSDGPSAGAAMAVGFIAMFKGDRVQRGTALTGTIEAGGQIGWVGGIPDKI
                     RAAKREGYHTVLVPRGQVHTPQWNLNELGFQLNIAVKEVDTIDEAYQIMTGSRL"
     gene            24756..25817
                     /locus_tag="VLD60_04375"
     CDS             24756..25817
                     /locus_tag="VLD60_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSB44223.1"
                     /translation="MLDRSSLTILIGLLVFSGSGAALAAPDCLVEEHGGGAQHLFKIH
                     LNAGCTEADREARAVDASKILAAVKQGEAIDVSGVVIHGGLVFDTLPVIANPPAVEGV
                     TLQQEAEVRVVTGAVSIVNSIVRGAVRHQSTQGILLFRGPVTLTGTTFEQPVDLSRSV
                     FAQPVTLSGAIFLRESYFVQGQFVREVISEKTAYGPHTRFHRSQFHGPVTFQQSGFSG
                     LAEFLEVVFDRDANFSRTYFKMGTGFSGGEFKGLADFSEALFDGDAFFTFTRFDGDAF
                     FRRATFRATADFDDAQFNAREDFSKVFFEKGPQFTRAKRSAQGSQSLGIENPQVQYVI
                     TLSLLLFSALLIAYLIRSR"
     gene            25894..26688
                     /locus_tag="VLD60_04380"
     CDS             25894..26688
                     /locus_tag="VLD60_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSB44224.1"
                     /translation="MDNQLDGAEQTELPYQVRIENFEGPLDLLLHLIKKNEINIYDIP
                     VHLIARQYLEYIEAMKDLNLTVAGEFLVMAATLLQIKSKMLLPVDEAAEDELEGPDPR
                     EELVRRLLEYKTFKEAARQLDTQERMWRDIYTRTPIDSEDLETDEQSLENVGLFDLVD
                     ALQGVLKRHPGKQLIEIIPDNLTVRDRMNVILEALEGQESVSFVSLFEASCHRLVIIV
                     TFLALLELIRLRTARVFQAESFGPILVSRAFSLVPDPAELDDSEWR"
     gene            26691..27560
                     /gene="scpB"
                     /locus_tag="VLD60_04385"
     CDS             26691..27560
                     /gene="scpB"
                     /locus_tag="VLD60_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSB44225.1"
                     /translation="MSATELQGAEVENVNVMETVSAEQDNPAVGHEINGNGTASANAQ
                     AEAFQGQELAAVLEALLFVSAEPVPLPRLASVIGTVSKAEVEQALKILQQQLEQEGRG
                     IQLVKLAGGYRLVTKPDYAPWLKRLDKAKAAQKLSRSALESLAIIAYKQPLVRAEIEE
                     IRGVETSGVIRTLLERKLVRIVGRKEVPGRPIMYGTTKFFLEHFGLQDLSQLPPLREF
                     KELGEAEQALLPIEDTTVVDVEPSEDMVSQEGAAESAILDSEERDAVLVGGASTMEAQ
                     LHEPLLGEPVEQA"
     gene            27812..30781
                     /locus_tag="VLD60_04390"
     CDS             27812..30781
                     /locus_tag="VLD60_04390"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HSB44226.1"
                     /translation="MIKAWLMDEMFRFDRAYLTVEGSQGEWGAGDSIAEEEELPPAPA
                     NVAAKCGNGRITITWDPVPDAMYYNLYFQTTKGVQIKFSELTRPIAGEDDFKAVIGVK
                     KEKATCLEGVQSPFVHDDLANGTCYHYVVTVVTPKGESPESQEVMAIPSPYLLAMSLG
                     QEGVDDGEFSSPTGVALDKDGNIYVADTDNHSIQKFDKTGKFLARWGSEPSSQEGSFY
                     YPRGLAIGANDTLYVADSGNNRVQKFDLEGNVMQAWGKFGFAWRGADMGKFDVPWGLA
                     TDQEGNLYVSDTSNARIQKFKADGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNIYVAD
                     ESNNRIQKFDTRGSFLTKWGREGAGPGQFKSPWGIACDALGNVYVVDTGNHRIQKFDG
                     NGTFLCAWGNRGKTEGQLNFPYGIAVDKEGCVYVVDSGNNRILKYVPTDEELNRGKDE
                     SPKSLEPNTTPPPHSVAVKAGDTEAFLSWMEVPGAVSYNLYFNTTPQLTIQSATKIEG
                     VTNPFAHTGLTNDVPYFYAVTAVFEDGTESALSNEVTATPVLIDITAPQNPYAVMNHG
                     AFMTNSPEVVVTISANDIDTGVGAYFISEAPMTPMAGTPGWVDVTPAYKFGATIPFIL
                     SPGDGQKSIYVWFKDVGGNVSTPASTSILVNTSGYLCVSKWGRTGRGASLLHGGEFMA
                     PLYGLCVDQQGSLFVVDNGNNRVQKFDNTGNFIILWGNFGSANANFHNPTGIACDGKG
                     DVWVVDTNNHRVQKFDGKLGGYLMKFGSRGNGEGQFNSPWGIAVDRVRGYVYVVDSAN
                     FRVQKFDMTGEFIMSWGSFGNGDGQFYFPRGIAIDQTDGSVYVVDMGNHRIQKFDTST
                     NVLPQLLTKWGGSSEPGHASSPLAQEAGQLRSPWGIAVDSAGDVFVTDTGNHRIEKFD
                     KEGNFITQWGGYGNGDGQFNFPYGISVDAKGSVFVVDSGNTRVQQFMPADEGSERLQE
                     EAEASAEIEKAQGTAST"
     gene            30806..32272
                     /gene="guaB"
                     /locus_tag="VLD60_04395"
     CDS             30806..32272
                     /gene="guaB"
                     /locus_tag="VLD60_04395"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSB44227.1"
                     /translation="MLDKEPRLGLTYDDVVLVPAKSAILPSEVELCTSLTRNIQINIP
                     VISSAMDTVTESRLAIAMAREGGLGIIHRVLSPADQAAEVDRVKKSESGMILDPITIS
                     PDGTIRDVYELMSKYRISGIPVTKNKKLVGILTNRDLRFETRMDLKVSQVMKRDKLIT
                     APEGTSLEKAREILHEHRIEKLPVVNKQGELKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAAVGVGPDTEDRVVQLKKAGVDLIVVDTAHGHSQAVLDTVKMIKKQHPTMELVAGN
                     IATAEAARELLKAGVDAVKVGVGPGSICTTRIVSGSGMPQLTAIADCAKALEGSGVPI
                     IADGGIKFSGDISKALAAGASSVMLGGLLAGTEESPGETVLYQARTYKVYRGMGSIGA
                     MERGGGDRYGQGGRPAKKLVPEGIEGRVPYKGPLSAVIYQLVGGVKSGMGYCGCKTIP
                     ELQQKATFIRQTVAGLRESHVHDVIITKEAPNYRMDWE"
     gene            32381..33937
                     /gene="guaA"
                     /locus_tag="VLD60_04400"
     CDS             32381..33937
                     /gene="guaA"
                     /locus_tag="VLD60_04400"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSB44228.1"
                     /translation="MELWHNRILVLDFGSQYTQLIARRIREAQVYSQILPCTAPLATI
                     LAYRPQGIILSGGPSSVYEKKAPTISKELFDQGIPILGICYGMQLVTHLSGGDVAKSK
                     HREYGRADLTIDDASDLFKGIGTGGSTVVWMSHGDRIERMPSGFRSIAHTSNSPIAAM
                     KRNDHKRRIYCLQFHPEVAHTAEGTQMLRNFVYDICGCKPTWTMQSYVESAVLQIREQ
                     VGKERVICALSGGVDSSVAAALTHKAIGDQLTCIFVDNGVLRAGERDQVQKTFASQMH
                     LNVRILDRTTQFLAGLNNVTDPERKRKIIGRQFIKNFEEESRKLKGIKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPARMKLKLIEPLRELFKDEVRVLGKELGLPDEIIW
                     RQPFPGPGLAIRVLGSINSERLAILRAAETIVDQEIRGAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYENVIAIRAVTSLDGMTADWAKIPNEVLGRMSNRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            34010..>34142
                     /locus_tag="VLD60_04405"
     CDS             34010..>34142
                     /locus_tag="VLD60_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HSB44229.1"
                     /translation="MEIRLQKLIAGAGLASRRKAEALIAAGRVTVNGKVVTELGTKVD
                     "
BASE COUNT         7342 a         8975 c        10614 g         7211 t
ORIGIN      
        1 gcgattcggc catccgacgt tgatcggaac tacgacgtcg ctggatgagc tcatggaaac
       61 gctggcgcag taacattcac agcagcggga tgaaggacag gttatgacga caggcagcat
      121 gctgtacgag gggaaggcga aaaaaatctt cgcgaccgat aagccggatc aggtcgtcca
      181 ctatttcaag gacgacgcga cggccttcaa tgcccaaaag cgcgggacga tcgttgaaaa
      241 gggaatcatc aataataagg tgtcggagcg actcttccgg ttgctggaac agaatggcgt
      301 accgacgcat ttcgtgcagc gcctcagcga ccgtgaaatg ctcacgaaga aagtcacgat
      361 cgtgccggtc gaagtggttg tgcgcaacgt tgtggcaggc agtctggcga agcggctggg
      421 cttgaaagaa ggcgatccga tccagccggc tatcgtcgag tggtactaca agaatgatgc
      481 gctgggtgat cctctcattg cagacgagca cgttcgattg ctcaagctgg ccacgccgga
      541 gcagatgggt gagatcaggc ggatggcgct gaaagtgaac ggcctgctcc agccgttctt
      601 tgccgagcgc aagatgattc tggtcgactt caaactcgag ttcggcttgc acaacgggca
      661 gttgattctg gcggacgaaa tttcgccgga tacctgccgg ttctgggatc agacgacgaa
      721 ggagtcgatg gacaaggatc ggtttcggaa ggatttgggg aagattgaag aggcgtatca
      781 ggaggtgttg aagcgggtgt gcgggtagca gaggcctgag ccttgcccgc tcgaacactt
      841 caactcgacc gcactatgga tatcgggggc gggtcctgtc gtcgatcatc gctcgtcctc
      901 gcaccccgcc cggagtagga accccgctgc gggcgagcga ttgatcgacg ccacccgatt
      961 agaggagaag gtggggtgca gtagcccgag ccttcgtgca agtcgtcttg aaagttcagc
     1021 ccagccttcc tcaactgtgg ggggaagagg aaaaaggaag agatgctgcg ggcgtttttg
     1081 aattacgggc tggtggcggc ggtggtggtc tgggcggcgg tggtcggcat gatggcctat
     1141 cgcctggagg aatcaccctg gcgctgggcg ttcatcgcgc tggtgtttgc cggtgggctg
     1201 accgtcgcgg gaattttctg gatcaggcgc tacgtcaacg cgttgaacaa ggcatcggaa
     1261 gagcagggga agccataata gaagagacgg cgtatgtcca aggatgttca aagggtcgtc
     1321 cggcaatgcc gcagcgagcg aaggagcgag gaggtaccta ccaagcttcg cttgagccgc
     1381 tcgcttcgat cacatgggag cggatagtta ctttgcctca agtcagcttt tgtatacgtt
     1441 gaccttctga gcgatgcaag aacgaagcat ggtgagaagt gcgtctcggc gcacagggtg
     1501 ggtgggtgag aacaaggtct tttcaacatc cgctgaggag gaataggtga aagccaagat
     1561 tcatgtgacg ttgaagcagg gaatcttaga tccgcagggc aaggcgatcg agcatgcgct
     1621 cgattcgctg ggcttcaaga atgccgcgaa tgtccgtgtc ggcaagtaca tggaagtaga
     1681 tgtcaatgaa acggataagg cgaaggccga tgcccaggtc aaatcaatgt gtgaaaagct
     1741 tctcgccaac acgatcattg aagaatatcg gtacgaactg agcgcgtgag gcgtgaaacg
     1801 tcaggggtga ggcgaaaagg cgaggtgcga tgaagatcgg agtggtggtt tttcccggaa
     1861 gcaattgcga tcacgactgc cagcatgtct ttcgagatgt gctggggcag tctgtggaga
     1921 tgatctggca caaggaaacc ttcttggccg ggctcgatgc gattgtggtt ccgggtgggt
     1981 tttcgtacgg tgactacctg cggactggcg cgatcgcgcg gttttccccg gtaatggggg
     2041 cggtaaagga gttcgccaag aagggtggtt tggtcatcgg catctgcaac ggttttcaga
     2101 tcctcttgga agcgggactt ttgccaggcg cgatgttgcg caaccgctca ctgcatttca
     2161 tttgtaagga cgtgtatgtg cgggtggaga atgcggcgac gccgttcacg agtgcctgca
     2221 agcccggtca agtcctgaag atcccgattg cccatgcgga agggaactac tataccgacc
     2281 cggtgacgct ggcggggttg caggcgaatg cccaagtcgt atttcgctac tgcactgcgg
     2341 acggcgaggt cacgccggag gcgaatccga acggctcgct cgataacatc gccgggattc
     2401 gcaatgccga agggaatgtg ttgggcatga tgccgcatcc ggagcgctgc gcggaagaca
     2461 tgctgggtaa cgaagacggg agaatcatct tcctgtcaat gatcgattcg ctgaaaaaag
     2521 ctacatcacc gctcagtggc gttggatcat gaaggccacg aaccccacca ttattaccaa
     2581 agatctcatc gcccagcata atctgacgga cgacgagtat aagaaaatcg tcgagatact
     2641 cggtcgcgag ccgaatctga cggagctggg catgttttcc gttatgtggt cggaacattg
     2701 ttcctataag agttcgcgcg tgcatttgag aaagttgccg acgacggggc cgcgagtcgt
     2761 gcaaggtccc ggcgagaatg ccggcgcggt ggatatcggc gacgggctct gcgtcgtgtt
     2821 taagatggag tcgcacaacc atccttcgtt tatcgagcct taccagggtg ccgcgacggg
     2881 agtcggcgga attttgcgcg atatcttcac gatgggggcg cggccgatcg cactgttgga
     2941 ttcgctccgc ttcggccaac tggatacggc gaaaaatcgc catctgatga agggcgtcgt
     3001 ggccggcatt gccggatacg ggaactgcat gggtgtgccg acggtcggcg gtgaggtgcg
     3061 gttcgacgat atctattcgc tcaatccgct ggtcaacgcc ttttgtctcg gcgttgcgca
     3121 caaggacaag attttccgag gcaccgccgc cggtgtcggc aacccggtga tttatttcgg
     3181 ctcgaagacg ggccgcgacg gaattcatgg agcgacgatg gcgtcagatt cgttcgatga
     3241 ccagtctgaa cagaaacggc cgaccgttca ggttggcgat ccgtttcagg agaaactgct
     3301 gctcgaggcc tgcctcgagc ttatggcagg agatctgctg gtcgggattc aggatatggg
     3361 cgcggccggt ttgacgagtt cctcctgcga aatggcttcg cgtgcgggca acggcatcga
     3421 actggatctt acggatgtgc cgcgccgcga accaggcatg acgccctatg agttgatgct
     3481 gtcggagtcg caagagcgca tgctgatggt ggcgaaggcg gggaaggaag aggagtgcat
     3541 caggatctgc aagaagtggg atctcgatgt ggcggtggtc gggaaggtga ccggcgacgg
     3601 gttgctgcgg gtcatcgacc agggcaaggt ggttgcggag atcccggcga agtcgctggc
     3661 cgacgatggt ccgcggtacg aacggcccta ctcgccgccg gcctatcacg atatgctgac
     3721 gaatctgaat tacgatgcca ttccggatgt gaaggccccg aatgcggcgt tgttgtcgtt
     3781 gttggagtcg cccacgatcg cgagcaagcg gtgggtgtat gaacagtacg atcatatggt
     3841 acgaacgaat acgatggtcc gtcccggctc cgatgccgcg gttatgcgca tcaagggtac
     3901 gaacaaggcg gttgcgatga ccgtggattg caacgggcgc tactgcctgc tgcatccgta
     3961 cgaaggggcg aggctcgcgg ttgtcgaggc agcgagaaat ctggtctgtt caggtgcgga
     4021 acccatcggc ctgaccgatt gtttaaattt cgggaatccc gagcggccgg acatcatgtg
     4081 gcagtttgtg ctggcaatcg aggggatgaa agacgcctgt gagcatttca agattccgat
     4141 cgtgagcggc aacgtgagtt tctacaacga gactaacggg ctctcgatct atccgacccc
     4201 catgctgggc atggtcgggc tcatcgagca agtggaccgg gcgatgaccc agtggttcaa
     4261 agacgaaggg gacgtcattc tcttgttggg gaagaccagg gacgatctgg gcgggagtga
     4321 atacctgaga gttcttcacc atcgagaaca ggggtcccca ccgtttctga gccttgaaac
     4381 cgagaaggcg gttcaagatt tcatactgaa agtgattcaa gaggggctgg ttcaatccgc
     4441 ccatgattgt tcggatggcg gtttggcagt tgcactggct gaatgctgtg tctccgcacc
     4501 ggacgtaaag cggggggctg tgataagatt gactctcgag tcgttgcgcc gcgatgccct
     4561 cttgttcgga gaaagccagt cccgcgtcgt gctctcggtg aagccggaac atgccgaatc
     4621 gattctcagc cgggcgtggg atggcggcat tccggcgatg aaaatcggga ccgccggcgg
     4681 ggagcggttc gtgatagacg tcgagaaggg gcaatggagc gacgggtgcc gccttgatct
     4741 gccgatcgac cagttgtatg accgatgggc tttttcaatc gaacgtacgc tgaatcaagc
     4801 gtaaggggtt aggcgtaagg ggtaaggtgt catgagtcag aaccaaggat ttcttcctca
     4861 cgcctcacgc ctcacgcctc acaccttacc catgatcagt cctgataagt ttcacgacga
     4921 atgcgccgtg tttgggattt tcgggcacaa agaagcggcg aatctgacct acctcgggct
     4981 ctatgctctg cagcatcgcg gacaggaagc ttccggcatc gtgtccggcg atggcgaaca
     5041 gttccatgcg cataaaggca tggggctggt agccgacatc tttaacgact cgatactcaa
     5101 gaacttggtc gggacgacgg cgatcgggca taaccggtac tcgaccgcag gaggccagga
     5161 tctcaagaac gtgcagcctc tcaccgtaaa tttcgccttc ggcaatctcg cactcgcgca
     5221 caacggcaat ctgatcaatg ctcaggtgct gcgcaatgaa ctcgaagcct atggcgcgat
     5281 tttccagtcg acctctgata gcgaagtcat catccatctt atcgcccact cacgagctga
     5341 tacgttgctg gcccgcatca tcgacgcact cagccaggtt cgcggcgcgt tttccgtcgt
     5401 gctgctgacc gatgacggca tcatcgcggc gcgtgatccg cacggattca ggcctctctg
     5461 tctcgggcga ttgcgcgaca gctatttggt ggcctcggaa acctgtgcgt ttgatctatt
     5521 ggatgccgaa ctcattcgag agatcgaacc gggagaattg gtcgtcctca atgagcaggg
     5581 cgtcacgagc tataagccgt ttgctccagt ccaacctgca atgtgtgtgt tcgaatatgt
     5641 gtacttcgcg agacccgaca gcaaaatctt cggtgccaat gccgtctatg ccacaaggaa
     5701 agcattgggg cgacaactcg cgcaggaatc gtgggtgccg gcggacatcg tgattccggt
     5761 tcccgattcc ggtgttccgg cggcgctggg ctatgcggaa gggggaggga ttccattcga
     5821 gacgggactg attcgcaatc actacgtggg acggaccttt atcgagcctg aacagtccat
     5881 tcgccatttc ggcgtgaaag tgaagctgaa tgccgtgccg gaggttctgc acgggaaacg
     5941 ggtggtggtt gtggacgatt cgcttgtgcg gggcacgacc agcagaaaaa ttgtgaaaat
     6001 gatccgtcaa gccggtgcaa aagaagtcca cgtgcgcatc agttcgccgc cgattatctc
     6061 gccctgtttc tacgggatcg atacgccgac gaaaaaagaa ctgatcgcgt cgagtcactc
     6121 gacggaggaa attcgcaagt acatcacggc ggacagcctg gcctatttga gccttgaggg
     6181 catgctgaaa tccgctcccg gtacgcccgg tcaatactgc aacgcctgct ttaccgaacg
     6241 gtaccccatc tcgttcacca gggccgaaga attccagctc ggtctcttcg aacggtctac
     6301 gtagttcgct tcagtcctcc tgctccgatt tccctgcctc gccgtctacg cagatcacca
     6361 cgacattgct agggtcgtta aggcttttcc cctctgatcg ctcagggccg ttacgcactg
     6421 tattccccaa aagtaggggg aagctcccag ctttcaggga actaggcagg aatccggcat
     6481 aggcataatg tgaggcggga aacggcaagc cgcggagact gctgtatgtg gtgcggatgt
     6541 cgagcaatca acgattcggg aggtgtgatg cggcaaccgt tcagtacgcc cgatcatgag
     6601 gccgaggttg cggcgatgac catacagcgg gaagagttat ctcagggtga ggattggggc
     6661 aggctgaggg aattggtgcc agttattcat ccgaaggttc aaggtgtggc gttagaaggc
     6721 gagaccgtcc tcttcgatct tcgtaccggg cggtcttatc gactgaatcg tgtagggaca
     6781 gctatctggg aacagtgttc tggcaccgct acgctcgaag agattcaccg ctcagtgtgt
     6841 gcccacctca atctgtcgct cgagcgagtg catgaccatg tgctgtcgtt cgttgcgcaa
     6901 tggagccacg agggactcct cactcacgga ttctctgcca aacaataaga tgtctggatt
     6961 atcagtgtgc agggagaggc aggagcacag ccgccgatga ccatagccgt acggcccctc
     7021 gaatatcgat tctcgtttca tggtgtcggg ctgcgctatc tgacgtcttc gcctaccttg
     7081 cgcgcatcca tggcggagtg gctcaaacat tttcgagggg atgggcccac agaaggcgtc
     7141 tcactgacga tccacttcga ggaagcccct ggtcgtgacg cggtgccgct cgctctctca
     7201 cctgccgcac gccgtctgtt ttccggcacc aagcctgtca tggggagcgc catgagagcg
     7261 ctctggcagt gcgatattct gcaggacggc agccatctcg ttgtcgacat ccatgaccag
     7321 gggctgctgg acatagacgg agcgcagggc acggctcgag gctatttcta tcatcccgct
     7381 gcgatgcatc aggaccggct ggaaggtttt tttcactatg ccttggccga actgctcaag
     7441 cggaacaaca tgttcacgct gcacgccacc gcgctggagt atcacggccg aggattgttg
     7501 attctcggaa atactggatg tggaaagacg accaccctgc tctcactgct ccgttccgga
     7561 taccgctatc tttccgatga tcacccggtt ctctgggatt ccggcacgcg attggagttg
     7621 cgagccgctc ccatgaagat cgatgtgacg gagcagacga tcgggttctt tccagaactg
     7681 cgcgacgcca tgtccggcct gctgcgccag ggagcctata aaaagtcctt ccaattggaa
     7741 gacatctact cagactcggc ggggcggtcc tgcgagccaa ctatgatcct gtttcctcac
     7801 gttacccata tgccccacag ttgtctggag ccgctcccca agagccgagc gcttgagttg
     7861 tttatgcccg aacagtcgct tgtgtacgac gaagaggctg ccgcacgaga gtttcaggcg
     7921 ctctcaaagc tggtgcagca ggcggcttgt taccggttgc atttcggcca agacgtgttg
     7981 gacctgcctc agctgatcac gccgttactc gaacgtcact gaagccgcca gtttctctct
     8041 gagttcgtcc tgctcgcgcg gatcggtgcg tgagctcgcc tcgcgtgccg ttgctcctga
     8101 aaaaatctgc atggtaggat acccggcaga agtcctgagt tctgagtgcc gagtcctgtg
     8161 aaatgaggga ctcagcacag cgtactcagc attcagcgct tcataagaaa tggaggcccc
     8221 tatgaaacgt cttgccctct tcctcagtgt cctcatattt ccggtctttg ccgcccatct
     8281 gctgttggct gcaggcttct ttaaagtcgg agagaaagcc ccgtcgtttt cgttgaatgc
     8341 cattaccgga gaggcggtat ctctggatgc ctacaaagga aaggtgattg tattggggct
     8401 cttccacatt tgcgaccctt gcatgatgca ggggagcaat ctccagaaag tgcacgaagc
     8461 gatgcaaggc aagaatgtgg ccgtgatcgg ggtgaattcc aatggcaatt ccaagaagga
     8521 tgtcggcgaa tttctttccg cgttcccggt gaaggtgacc tatccctatc tgttggatcc
     8581 gggcaaagtc acggacaagc tttatggcgg ggggaaattc attcccaacg tctatgtcat
     8641 cgatcagaac ggcatcatcc gctggcagcg tgtgggcaat atggaccttg ccggtgcgga
     8701 tgtgattgtc gccgaggtag aaaagttgtt ggcgggcgga agcagcaaga tgtgagagcg
     8761 cgtcggctag gggttccgct gctcgcggaa cgcgcgccct cagaaggaat tcgttcgacg
     8821 cgcgcatact ggaaccccat ggccgccacg cataaggaag atgccactgg ccgcacgagg
     8881 aggagaggta ggaagagata tggaagagtt ggaagagggg aagcagaagt tcttggaggt
     8941 ggtgaaagcc attgatgagg gagttcatgt cgtcattcct gtgactccgt ccaacagcat
     9001 gttcctgatt tcgctgacca aggggtcgaa tcggaaattc atcaccgtgc cggaagacga
     9061 catcatcgat cttccgaacg aggccggtat ccttgccaaa gtcaccaaga cggtaaagga
     9121 cgcggtcgcc tcactgtaaa tcatgatgaa acaagtcctc ccgggattct ggcagtggtc
     9181 ctggttttcc gaggaaaagc agctcgactt caacggcttg ttcctcgtcg tgggagaaca
     9241 caaaattctg atcgacccgc ctcctattgc aggcgaggat caatcgttca ttcgacggaa
     9301 cggaccagtc gactatctca tcgtgacgaa ccgtgaccat gcgcgtgagg ccgggccgtg
     9361 ccagggaaat ttcggctgcc aactatttgt accagaggcc gacgcgtcac aaatggatct
     9421 caaaccgacg aagacgttca aagatcagga attgctgcct ggcggcctat gggtggtgca
     9481 tttgaaagac caaaagtctc ccggcgaatc ggccctcttc attcagctcg gcaaaggaat
     9541 cctcatcgtg ggtgacgccc tcatcggaaa accggccggg gcgctcagca tgttgccggc
     9601 tgaaaagtat gcagatgcgg caaaagccaa ggaagggctt cggcgtctcc tgaagtacaa
     9661 cttcgatgcg atgcttgtcg gtgacggcac gtcgattctc acgggtgcca agcaagcggt
     9721 ggaacgggcg ttacagtagc gagtgactgg tatgacctta cgaaacggcc tgtccgaaga
     9781 actcaatcgc caagggaacg agcacttcgg ccgcggccac tacacggatg cctatacctg
     9841 ctatgccaag gcgctggaat gcgatcgcat caccggagac cagcgggccc ttgcggcgac
     9901 actcggcaat ctggggaaca tttgcgcggt gagcggacgg cgcgaggcgg ctcaaaatta
     9961 ttatcatgaa gtgttggagt tgcagaagat cctgggcgat gagaaaggga tcggcaccac
    10021 gctcgcgaat ttgggcaatc tgcgcgcaga cgcgggcgag tgggaccggg ccagggccta
    10081 ctacttagag gcccttgatc tcatggcgaa gtcccatgac gaggccgcga aggccgtcct
    10141 gttttccgat cttggactgg tcgcgcggga aaccggaaaa tttgacgacg cgatcaccta
    10201 ctatgagaat tctctcgtct tgatgcggcg cttgggcaat gacggcggag ccgcagatgc
    10261 ctggcggatg atggcaagga cgtttctgat gcagcaccgc tatgaggatg cgatcgcctg
    10321 ctgccagacg agtcagtcca tcgccgaacg ggcgcgcgat gagctccgcg caggcggggc
    10381 tcgctacgtc ctagcggact gttatgagtc aatgggacgg ctcgaagaag cggccaactt
    10441 gttggagcgg gttgtggaca tggaccgcaa gtatcatctg cccaagctcg aggaaaatac
    10501 cagacggctg caggcattgc gggatcggat atgcgacgcg tctagtcccc caaccctccg
    10561 ggagtcgcac ggatgagcga gcaagcctca gtattgtgga ccgaggtgcg ccgctcgctg
    10621 tcccaagctt ttccgcaatt ctatgagttg gagccaggag gtccgcttgc gctggatctc
    10681 ggcaacgacg gctggctttt ggaaatcaag ccggaaggca agctgctctg tcagtatggc
    10741 gtggcgatgg acgatgtgat gagcttgatg tcagaaggga ccccggagga tttgggcaca
    10801 gacgaagtgg cgaagcaggc gaagtacttt attcaaccgg cggtttcgaa atatcgcccg
    10861 ctgctattgc aatccgggtt ttccgagcaa acagacatga acgaggagta tgtggcggtg
    10921 gcattcgaac gcacggtcga cttcgagaac ctttcgaaag tgaaagacct catacgttgg
    10981 tgctgccggc agattggcgg gtcggcatga tgcgagcaat caagtccttt gacgactttc
    11041 gcgacaccgt ctcggcctat cgtctgccgc gggtgatcct cacggcgctg gagttgaagc
    11101 tctttacagt cgtcggggca cgaatatgga ggatgtccga ccttgctcgc gaactgaagg
    11161 tgagcgagcg agggctttct atcctctgtc gaaacctcgc catggccgga ttgctgcaca
    11221 agaaggggga cctgtatcgc aacagccgac tcgcggcgac ggcgttgaac gccgatgatc
    11281 ccgcctatcg aggaaattat ctgaacctta tcacaagcca ctgggcggat tggatgcgac
    11341 tgacggaatc ggtcaggacc ggtgaatcgc tcgataaaga cgagccggag gcgcaggact
    11401 atcgccgcca attcacctgg gcgatgcacc atcgcacgct ggaaaccgcg ccgaaaatcg
    11461 ccgcacagat ttccctgaaa ggggccaaaa cactgctgga tctcggtggg gggcccggga
    11521 cctatgcgat ggcattcatg gctaacaatc ccggcctccg tgcgacggtg tgtgatcgcc
    11581 ccgccgcgtt ggaagtggcc aaggagattg cggcgacgca caaggccggt ccccggctct
    11641 cctatttacc gttggacctg ttgaaggagg atattccggg atcgttcgac atgatctggt
    11701 actccaacgt cttgcacatc tattcgccgg aacagaatca ggcggtgttt caacgtgcga
    11761 agaaggcgct ggtcccaggg ggacgcctca tcatacaaga cgcattcctg catgatcgag
    11821 aaggcctctt tccagaagag gcgagtctat tcgctgtctc catgctgctt ttcacagaga
    11881 ccggcaatac ctattccgct gccgagacgg cgaggtggtt gaaagaagca gggtttggag
    11941 cgatcaggat gctgaagatg aaacagggga tggaggattg ggagggtggg atcctggagg
    12001 cggtggtccc tgtaggcaac tccagaacgc acggccgcca aaaacgatca acgagaagtt
    12061 cacgatcccg ctgattccgc tcagtacgag tatcttgtct gttcccccat gccctccaag
    12121 aatgctcgtc acggttgcca gatccaacgc cagtccgatc gtgccgtaga tgacgcaggc
    12181 catgggggtc catctccatc ccacccagac cacgctaccg agtgccagag gcaggccgac
    12241 aagagtacac agccacaaga atcgagcagt cggcgaaagg tctggtgaaa gggctaactg
    12301 gactgtcgcg agtccgatgg cgctcaggac acacaacagc agaagcaatg ttcctcgata
    12361 ttgcatgccg gcgatcatac ctgtgacttc acacgtgagc aacgaggtgt tttaaacgga
    12421 gccctggcca tgaccgatcc ttctcctgat catgaccccc gaccctgtct tttgatttat
    12481 gatggcgaat gccgcctctg cgtgaccgcg aagaagggga tcgagcagct gcagtctgaa
    12541 actgcaggca gctcactacg tatgattacg tatcagagcg aggaggccaa gcaggctctc
    12601 ggcatgcgat atcgcccagg ccgcccggac gtcgcctttc tcgtggaccc ggatggcaag
    12661 gttgcgaaag ggctcgatgc atttctcccc ctgcttccgg gactgaaggg aggccgggtc
    12721 ctcgcgagag tactcgcatt ccccctggca aaaccgctag gtgaccttct gtattggttc
    12781 gtggcgcggt accggtatcg tctgttcgga gtagttccac ttgagcgctg ctctgagccc
    12841 cagcagtcaa agaaggccga ctccatcgac agccatactc cttcgtcagg cggccctacg
    12901 aagttgccct agccagcttt ctgcacattt ccgttcacgg catatccccc tcttttgagg
    12961 aatttctccc cacagtgggg aatttccacg tcgcagcgta actgagatag ttaggcatca
    13021 ataagggggc accgctcatg aggttgatcg cctggtggtc agaggtaatc cgccaattga
    13081 aagaaggttt caggcttgtt caacgagacc cggttcccgt ctgctcccgc tcaaccggct
    13141 tcattcgacc catcaagact tatagcatgt tctacgtagc catcgttggg gcggccttgc
    13201 tcggtttgaa tagcgccaac ccggcctgga gtgcagatgc gagcacagag aaaaatagga
    13261 aaaccgtggc ggcgggtttg ggctatcaag acgccgacat ctcagcgatt accgtgaaga
    13321 cctacgatgc ggaatccggc gaagtccttt cggctgaaac gtacgaactc gatatcaagg
    13381 atgatgggcc tccgtcgagt cgcccgcgta ctcgtatctt cgcagggggt gtcggcgtcg
    13441 gttcggatgg cttatcggag ttcacgcttc gtgtgtacga tgcggcaaac gggcgtttcc
    13501 tctgggaagg gaggctgaac ctcagcagcg gcagcaatcc cgacgtcgcg gcctttccgg
    13561 tcgtggccca ggtccagtcc cgcactgcga tggtgaaggt caccggcagg accaaaacga
    13621 gcggccagcc ctattttgtc ctccgtgccg tgaaccctga aacagggcag ctggcgtggg
    13681 cggacgagtt ttccgccgat ccggcgaatg tgagagtgga gcgtatcagc cggagtgtga
    13741 tcggcatgac gggagtgatt ccgcgggaga tcgatttccg gatcaagatg ccggacgaag
    13801 ccggtcagca gttgttgtgg gaagacaagg tcgttccagc catcgatgaa gaggacgcca
    13861 taccggagcg atcggacgat tctgcgggca agctgccgat gtggccgcaa ggctccagcc
    13921 aacgtgtaga gagagacggg atttgatccc gctgtaagtg cgcagcgacc gttaagcgaa
    13981 gcttgctctt accccttctt ctgttcctcg tgcagttgct ccaaccaacg agattgaaac
    14041 tgctcgtacg agagcgagag ctgggactgc attgcgctcg tcaaggtttg attcttcttc
    14101 atgcgagcca gtagttgctg gacctcatgc atgccgtatc gacggatgag ataccgcgtt
    14161 gcagagttgg cttcaagata cgcgacggat gccgcttcgc tcgagagccc tccccaacca
    14221 ccttcgaggg ccgtcaacgg aagaaccggc acatctcctt gattgagctc ctccaggtct
    14281 gaccagcgat cactggagag ctgcatcgcc aacccttcgt tcagccaggt tggcagagtc
    14341 gtgtggtgtc cctgcaggtc ttggagcagc gcatgtacga attcgtggcg cagcacgcgt
    14401 tttagccaga cgcgatctgt caaggcgcct tgtgtgggga tctggatgcg accgaggatg
    14461 gaatcgaaca gtccatcggc ccatgccggg ctgcccgtga cgccttgaaa tgtctggttc
    14521 gcgtgcagga cgaccacaat cggccttgac gggaagtgcc cgaacttttg cccgatctcc
    14581 cggtaggctt cttccagaat ttccagcacc gcagtccagg tgtcgtgatc agtgcttccg
    14641 tcgtacttca cggtgaaatg agcgccgtcg cggctattca agcgggattc tgcctgctcg
    14701 gtccgatgga ctttggcagt cacggcacgg agatacgact gcaggcttgg atcgttcctc
    14761 gcccgatcgg cagcctgatc gaggtggggg gcagcttcgc tcatccggtc acgctcctgc
    14821 agcagatcgg cgagggccag gtgcggaaag ggttcatcgg gtgcgagcgc gatcaaccgt
    14881 tcaaggaatt cctggctcat ggcagggtcg cgttgttccc agtaggcatg ggccaagttc
    14941 atctggatca cggggttctt ggcatcgagc gccgcggcat tcttgaaggc tttgacggag
    15001 actctggtcc catcgtgctt ttcctgttcg atgcccaggt tattccagag ggtcgccaca
    15061 taggaacgag cgagggcatc ggactggacg gccgacggga gcctcaagag tttcgtttcg
    15121 gcttcgttga ggttgccctt ttcgatgtcg tctcgaatcg ttgccaagtc atcctggtac
    15181 ttcgtatccg gtacgttact cgtttcgatt gcgcgcgatt gcttgggcgc cggctctaat
    15241 tgttcaccgg attgcggttc cggatccgtc tcattgccgg attgggattc cccttcggtt
    15301 tgtatcggtg aaggaggatg tacgtatgcc ggcttcagcc aggcttcata cacgatgaag
    15361 agcccgagaa tgcaggctgc cggatagagg acgtgcgaga tattccgacg gtacataaat
    15421 gacgtatcct tgcttgcgct cagtgtaaca ccggtttctc ctacgccata gaattgtaag
    15481 gaaatctacc tggcgtccct ggtcattgac gccgaggtcc tcgttgaggc gcacgcgggc
    15541 ctgtgatacg atctcgcccg tgatgtggcg ctcttctgag cccaaacccg ccgagcaatt
    15601 tcaacaatgg ttggaccgtc tcgcacggcc catcgaattt gccagccgtg atgctttcgc
    15661 gcatctaccc actgtcaaga atctcagtag ttttgtctcc acacaggtga tgcaggccct
    15721 ttccgatcgg gtgtatccga gggcagtcga ggctgcattg ctcaagcttc gtgagttgtt
    15781 cggtgaggat cagcagcggt tgtcgcaagc ggaacaacaa cgccggcttc aggaggctac
    15841 cgccattcta aggaccttgc gccgcacggc cgatgatccc gctcgtgcct ggcaggatcc
    15901 ggagcccctc gcatggagtg cggccgtgac gaagagcgca ccgacgcacg aactgtgggc
    15961 gcaccccatt cgctttgcga agggcgtcgg tccaaagcgc atggcggtcc tgcagcggtt
    16021 acgtatcgag acggttgaag atgcgctctg gacggtgccc tggcggtatg aagatcgttc
    16081 ggtcatgacg ccaatcggcg agttggtgcc gggaatggcg acgtcaattt gtggtgaagt
    16141 ggtaaagagc gaggccaaac ggacgagaaa ccggcgtttc aacgtcctgg aagtctgtgt
    16201 cgaagatcgg acgggacgca tgcaggtggt gtttttcaat cagccgtatt tggagcaggt
    16261 attggccgtt gggaaaaccg tcatgatgag cgggcgcgtg atcgccggcc ggcagggctg
    16321 gatggttccc cggatggatg tggcgcagta cgaggtggtg ggtgaaggaa ccgaatccac
    16381 gttgcacgtc ggtcggatag tgccgattta tcacgaaaca aaggggtgga cttcacggca
    16441 gatgcgcgtg ctgatgaagt gcctgctcga tgagcatgcc ggggatctga gggatcagtt
    16501 gcctgtgccg gttcgtgccc gccagcgcct ggttcccatc caggaagcgc tacaagacat
    16561 ccattttcca aaagagggcg ctgagttgca gctgctcgag caagggaagt cgccggcgca
    16621 tcgccggctg gcgttcgagg aactctttct cctgcaagtc gcgttggccg cgcgccagcg
    16681 ttccgtccag agtgagaaaa aaggtctgca cttcagcccg agaacgccct tgttggaaaa
    16741 actggggcgg ttgctgccgt tccaattgac tgcagcgcag gatcaggtga tccgggaaat
    16801 tttcagggac atgatttcgc cgcgcccgat gaatcggttg gtgcagggcg atgtgggatc
    16861 ggggaagacc gtcgtggcac tgcatgcgat cgtgatggcc tgcggctcgg gctatcaggc
    16921 ggcgcttatg gcgccgacag aaatcctagc cgaacagcat tatcgcaatt tgaagggcat
    16981 cctggagagc ctggggttga acgtagtttt gttgcgcggc ggagagaagg cggcactgaa
    17041 aaaggtacag gcgacgcagc ttgcgtctgg gcagattcag gtggcaatcg gcacgcatgc
    17101 gttaattcag aaaggcgtgg ttttcaagaa tttgggcctg gccgtggtag atgagcagca
    17161 taagttcggt gtcttgcaac ggaagacgct gaaggaaaaa ggctataagc cggatgtgct
    17221 cgtgctgacg gcgacaccga ttcctcgtac tctcgcaatg accgtgtacg gggatctgga
    17281 cgtctcgatc atacagatgc tgcctccggg acggaaaccg gttcgcacgt ttctcttcgg
    17341 tgaatcgcaa cggcggcgcg cctatcaaat cctgcgagat gagttgcgga acaagaaaca
    17401 ggcgtatgtg gtctatccat tggtcgagga gtcggagaaa gtcgatctgc aagccgctat
    17461 ccagggggcc gaattgctgc agaacggcga gtttaaggag tatcaagtcg gtctgttaca
    17521 tgggcgcatg aagccggcgg aaaaagaagc aatcatggcc gacttcaaaa ccggcaagat
    17581 tcatctgctc gtcgcgacga cgatcatcga ggtcggtgtc gatgtaccga acgcaaccat
    17641 catgatgatc gaacatgccg agcgtttcgg tctggcgcag ttgcatcaat tgcgcgggcg
    17701 tgtcggacgg gccggtcatc aatcctattg cctgctgatg gccgcgacga tggggcgcac
    17761 cagaacccag ctaggcaaac ggccgtcagg caatgaggaa tcgtcgaccg ccaaggaacg
    17821 gcttgaggcg ttggtccgat caaatgatgg gtttgtgatc gcagaagaag acttgcgcat
    17881 cagagggccc ggtgagtttt tcggatttcg ccaatggggg atgccggagt ttcgcgttgc
    17941 caacatcgtg agggacagtg atctgctgca gcaggcaagg ctggaagcat tctccttatt
    18001 gaagcaggac cctcagctga gcgcgccggc ccaccaaggg ctgcgcgaag cgatgttgag
    18061 aaaatgggaa aagaaattgg agttgggatc ggtgagttga gattcgcggg gactgtcggc
    18121 gcccaagccc attcgtcctc gcttcgccca tcccccagtg ggacccttcc gctgcgggcg
    18181 cttggtgaaa agcgcgtctc ggcgcggcaa gggtcgggcg ggtgataaag tcgcccggac
    18241 gccgccaccc gcgcagagga agaccgatcc caatggtaga gagaaagtag aggagaagat
    18301 gggactgttg cagcggttga agcatgattt gatgtccggg ctggcgacgt tacggctggg
    18361 aacggcgcaa gcggcaaacc gggcgctgga agaaacggag ttgctccgct tgcgtctcga
    18421 agttcgcaag atcggtcagc aactaaacga tctgtatcgc gatgtcggcg agcgggcggt
    18481 aagcttgcgg gaagccgggg agccggcgga gcgggtcctg tacgacatgg aaattggtcg
    18541 gctggtcaaa gaaatccatc aactaaagga agcgcgtaag aagttggaag atgacatggc
    18601 ggaaattcgg aatgccgaat gaacacgcaa cgcctggccg gtatcgatat cggcaccctg
    18661 acctgtcgcc tcctcattgc tgatctcccc tctcatgggc cgctcaaaga gcttcgatca
    18721 gagcgccgca tccttcggct tggcgaggga gtcgatcagt cgaagcggct gaatgcggct
    18781 gcgatggatc gggcgattca ctgcttgaag gaatggcgtg aagtgatcga cagctatcgc
    18841 gtcgatgcct gcacggcggt tgccacaagt gcggttcgcg atgcggggaa ccggagcgag
    18901 ttcttggagc gagtaaaacg cgaggccggt ttcgacgttg agattatcac aggcgaagtc
    18961 gaagccgagc ggacattgtt aggcattcgc tcggggttac cacatggcgt aaccgatatt
    19021 ctggcgctgg acatcggcgg tggcagtact gagttcatcc tcgatcgacc ggaacagaag
    19081 ccggttgtgc gatcgattga tatcggggtc gtgcgactct gtgaaaggat attgcgccac
    19141 gatcctccga ccgatgaaga aatccgccgg gcccgcgagt gggtagcggg agaaacgaaa
    19201 attgcggttg cggacatggg caattatcag acagcgacct tcgtcggcac agccggcacg
    19261 attacatcgc ttgctgccat ggcgcagaag cttcccgcct acgaaccggt cagaatccat
    19321 aattaccagt tgaagctcgg tgcgattcac gcactcgaac aggagctctt gagccgcaag
    19381 aagaccgacc gtatcggtct gcccggcttg gaggaaggcc gagaggaagt cattgtcgcc
    19441 ggtgcaatca ttatccgaac agtcatggag acgttaggga tgtcgagcgt acttgtcagc
    19501 gatttaggat tgcgggaagg tgtgctgatc gatttggcca ggcgggggcg atgagtcgca
    19561 gagtaaaaga ggcgccctct cggctcatgt tttacttctg cgaataccac cgatagcctt
    19621 tttgttcggt gaacgtcggc ttcggcgccc ccccgggttt ttccagcaac accactttga
    19681 agtcgatgag gccgaaccag gccgggtcta tcacgtcgtg gaaatgcgtg ccttggaact
    19741 tcggcaagag atcaccgagt gcctgtttga gttcgctttc agtgtaggcc cgcacggtga
    19801 aaggcttgtt cattgcctga cagaatcgtt ggatcgtata ggcggcgccg gtgcagagca
    19861 ctttcgtgtc cggcttgatc gccagaaact gcggcagcgt cagatagcgt tcctgaaacc
    19921 cgagccaacg gacggcttgg cgcaggtggc tgtattgcac ttcgtattcg gtatggcccg
    19981 gcagtttgac cggagcgggc cagccttctt cgatcccgaa ttctgtcaga aaggcgcggc
    20041 cgcctggttt taatacccgc cagagttctg ccaagaagcg aatcgggcct aagttaaaga
    20101 tcacctcgtc cggtggatgg gcttcgatgg ggaggcgcag ccgcctgatc caatcgagcg
    20161 cctcctgatg ctgttcggtg tcacccttgt tctcaaggag ttccttgctg gtgagtttga
    20221 ccggcgtcat atcggccatg ttctcgttgt cgatcacgag gtcgaccgac tggtctttga
    20281 aggggagtcc ttcagcgttc gcgcgggtgc cgataccgct ccagccgcct tccttggccc
    20341 gcgtcgtttg gagcttcaga aacggctgtg tgatatcgag cgacacgtac ttgatcgcct
    20401 ggcgctcgaa cgggaggatt tcttttgcca tctcccgagc cacgtacccg agcccgccgc
    20461 cgacttcgag gagaatcttc ggtttcggat tgaaccatcc gagcttgcgc aattgctgca
    20521 tgagcaagcg gccgtaggtc aggccgttga gcgcggcatt cggttcgcga aaaagatgtg
    20581 agacggtcgt ctcgatcaag tcaaagtgcc cgtcttcctg agtgctgtca gtcaattcat
    20641 ggtgatgaaa gtcttccagg tgttcttcgc cttcgaatcc ctcctggccg gaccagcctt
    20701 ctcggatttg ttggaggagc acgtcccact tggctttgtg ccgatggccg ccgggtggtt
    20761 cggtgccgaa gtagcagagc gaaaaattgg ggagcgccca acgttctaaa tgccaccggc
    20821 ctggttgtcc gtcaggtcca cacacgcgcg tgccatattt ttcgagcagg gcgccgacag
    20881 tcgtgtgacc gttcattgct ttcagcattt caagaccgat gcgattgact cgcacgatcg
    20941 ggaggttgtc gtcgagcggg gcgaaccacc attcgtcttc gtgatgttgg taggcgacga
    21001 gatcggggat atgccaggcg aggagcgtga gggtctctcc tgcgaggctg cgaggctcgt
    21061 gaacagcgac ggatggcttc atgtcggcaa tactatatga gcgtcgcagc ctacacgacg
    21121 ggccaggatt cctgcaacat ccttggaatg tgggttcgct tggggagggg tctcgttaga
    21181 gctgcgaggg aacaaagctg tcagtcacga tgagcatgcc tcgcatgatg ggatggacgc
    21241 ggcagaagta gggatagcgg cctggtgcga gacctggcaa tgtgaatgtc ccgttgggca
    21301 ggacgatacc ggaatcgaag gcgcagggct caccgtcctc catgcaaccc atgtgggtaa
    21361 tggtatgttc ggtgggggtc ggattttccc atctaatggg agctccggtc gaaacggtcg
    21421 ccaattttgg tacgaaatag ggagatccgc tgtcgataat gatggcggta tgaggcggcg
    21481 tagcagttgt aggccaaact gccaagaggc ccatggccag aacggcgatc gtcaagtgga
    21541 actttagtgt catcttcacc tgccccaggt ttcaactcta ttgctttaga gcttgatctt
    21601 cgcagaagga ctcatcgcat tccatgttga ttattttacc atggtagtcg acggatcaat
    21661 agggcgtctg ctgtccgcct gatactgcgg acttgacctg gaggcaggat tgtacgattc
    21721 tccgctgcat tccagggggc ggtatgcctg aggcgggcga gcgcagatca aaacgggggc
    21781 agcggttgtg cttgatgctc ctgtggacca gccttcaagg gcttctctgg tcaaccaccg
    21841 gagagacggt ctgggccatc actctgacga caccgatcca gggtgcaact ctgcaatccg
    21901 gcaagtcggt accggtggca gtgtccgttg ggaaagaggt cagtcttcgt gctgtacgct
    21961 actactggta ccggctcgat gaagagcctc ttgcatcaca ccaagcgaca gcggccccct
    22021 tcgttgcaac ggaagcagga gctccattct ccgggagtgt ggtggttcct gctgaagcct
    22081 tgggagcaat gcggctgctg gtcgttggag aagtggccag aggccgattg gaaagttacg
    22141 aggagttcga cgaggtgctg gtcaccgtcg agaccgcggc tgcgctgagc gccatcgaat
    22201 tcacggttca aaagccttgg cggttcgaca agatcggcaa acgggtgatg gttccggctg
    22261 tcggacagtt caacgacggg ttcgtgcgtt cactgacggg tccggacgtg gggagccgtt
    22321 ttcgctcctc tgatgatcgg gtggtttcga tcgatgctgc agggtgggtg caggtcttgg
    22381 gatccggtaa ggcatcgatt atcgtcgatc atcgtggaaa aagcgggacg ctggaggtcg
    22441 ttgtcgaagc cgatgaggag ccgaaccgac tcccgattgc cgaggtggcc aaagagctgc
    22501 aggtcaaatc gggagagatt gtcgtgctcg acggccttcg cagccgtgac ccggacggtg
    22561 atccgttgca ctatgaatgg aagcaagtgc gtggacatcg tgtgggcctc accaacgtca
    22621 atgaatccaa agcgactttc gtcgcgccga aggtgtctga gcgcaggatg taccagttct
    22681 ctctcgtcgt tacggatatg gcaggaccgg ataccatcaa gggagcggat agtgccccgg
    22741 ccgtgattac ggtctgggtc agcccgtgag atggagcgga gtggacctct tcatgcagag
    22801 tatacggtgg ccgtgaatgg ctgtcagtag aggctttgca agggatcttg gacgtgctgc
    22861 gcccccccgt tgcaacgtcc ggcaagttca tgctagatgg aggccgcatc agtctccacc
    22921 tgtttgtgga tgagagagga gcagatcgat ggcgaaaaag aagaggccgg caaagaaagg
    22981 aaccgccaaa gccaaggcga agggtgcggc aaagaaggcc gtgaagaaac ccgtcgcgaa
    23041 gaaggcggcc aagaagccgt ccgcagcaaa ggccaaaacc gcgccgaaga aaaaaggcgt
    23101 ggaaccgtct cgctcagcaa agggggcggc cgcagtcaca aaaacggcaa cagccgctct
    23161 tgacgaggag ccgaagaagg cgacgttgac gaaaccggct cctgcgatga tgcccaaaga
    23221 gcccagtctt gacgatgagg agatcggcga ggaagaactc gacatcggcg gcgaagaaga
    23281 gattgatgaa gatattgagg aagacctctc gctcgatgac gacgaagagg atgtcgacta
    23341 tctcgagaaa tctgaagact tgctcgacga cgccgatgat tatcgccatt gaccctctca
    23401 ggttcaacgg aatctaatgg tttccatcgc atcgccccgc ccctcgtaat cagtcttcaa
    23461 acagacagtc ccgcccgctg ttgtgttggt cgatccacat ggagacgtgc tcgtgagtca
    23521 ttttattttt atcgcggctg cgctgcgtga acggacctcg gaagagagtg cggagcttca
    23581 gctctccctt cacctctggg ggctctgcac ggccctcatt cgatcaaatc tgcaaacgta
    23641 tctgacgccg caatcgcgag ggctggtcta tgtcctgaaa gtcgggctat gcgccgaatt
    23701 tcatatcgcg tccccgccgc aggatttttc tgcattggat gactttctga aggatgagct
    23761 gcggacggag gcgcgctacg gattcgtgcg gatcgacggg attcgccgat ggacctggtc
    23821 ttcccaggca agccaagccc tgttgctacg ggtgcttggt attgcggatc aagcggagtt
    23881 gacgcgtcgg ctcacactgg ggatgcatcg gttgacgcaa gccgaatacg acgcgatggc
    23941 tcaaggaatt gccgagggag tcggacaggg ctagagacga ctgccggtca tgatctggta
    24001 cgcctcgtcg atcgtgtcca cttcttttac ggcgatgttc aattggaagc cgagttcatt
    24061 gaggttccat tgcggtgtgt ggacctgccc acggggcacg agcacagtgt ggtacccctc
    24121 tcgtttcgcc gcacggattt tgtccggaat cccgccgacc cagccgatct gaccacccgc
    24181 ttcgatcgtg ccggtcaacg cggtcccgcg ctgaacgcgg tcgcctttga acatcgcaat
    24241 gaagccgact gccatggcgg ctcccgcact cggtccatcg cttccggtcg gccgatacgt
    24301 gacgccaatg acggacacga cacccgtgtg ctggactgcg ggagttcgct cgacggcgta
    24361 gtcgaatgct tgagccatcg cgccaacgtg ggttttgccg aatcgtacac cgccttgcaa
    24421 ccattgtaag agggcgggat tggggtctgg aagcctgtcc catttgagca ggagtacctc
    24481 gaaaacgccc aggttattgt agggcacagc agcgaggacc ggcagttcaa taaggtcccc
    24541 tgcaagagtc gggaacggga tcgtgaaaac aaggacggtg atgaatgcga aggctgcaag
    24601 gccccattgt cgcatcgggc gcgctccaat tttggtggtc ttgtcgagtt gtctcagaga
    24661 cgtcattctg aagtttacca aatcatcagt cgccgtaaag tagaagggtc tttgctgtct
    24721 tgccatgggc tgaggcattc cgtataaggg ccttcatgct ggatcgttcc tccctcacta
    24781 ttttgattgg actgttggtc ttcagtgggt caggggctgc tcttgctgcg cccgattgtc
    24841 tcgttgagga gcatggggga ggtgctcaac acctcttcaa gattcatttg aacgcgggct
    24901 gtaccgaagc cgatcgtgag gcccgtgcgg ttgatgcttc gaagatactg gcagcggtga
    24961 agcagggaga ggcgatcgat gtttccggtg tggtgatcca tggaggtctg gtcttcgata
    25021 ccttgcccgt cattgccaac ccaccggcgg tcgaaggtgt gaccttgcaa caggaagcgg
    25081 aggtccgggt cgtgaccggt gcggtttcca tcgtcaactc gatcgtgcgc ggagcagtca
    25141 gacatcaatc aacgcagggt attctgctct ttcgcggtcc ggtcacgttg accggcacga
    25201 cattcgaaca gccggtcgat ctttcgcgat cggtgttcgc gcaaccggtc acgttgtccg
    25261 gggcgatatt tctgcgggag agttatttcg tgcaagggca atttgttcgc gaagtgatca
    25321 gcgaaaaaac ggcgtatggc ccccatacga gattccaccg ttcgcagttc catggtccgg
    25381 tgacttttca acagtctggc ttcagcggcc tggcggaatt tctcgaagtc gtcttcgacc
    25441 gtgacgcgaa cttctcgcgg acctatttca agatggggac gggattttcc ggcggcgaat
    25501 tcaaggggct ggcggatttt tccgaagccc ttttcgacgg cgacgcgttc tttaccttta
    25561 cgcgatttga cggcgacgcg ttcttccgcc gggcgacctt tcgtgccacg gcggatttcg
    25621 atgatgcgca gttcaatgcg cgcgaagatt tctccaaagt gttctttgag aagggaccgc
    25681 agttcacgag agcgaaacga tcggctcaag gatctcagtc gctcgggatt gaaaatcctc
    25741 aagtccagta tgtcatcaca ctttctttgc tccttttcag cgctctcctg atcgcctatc
    25801 tgattcgatc cagatagggc agcctcccct ccggaccaca actaatagat ccacgcttgc
    25861 tcagcccccc ccaaagctgg tataaagccc cttgtggata accagctcga cggtgccgaa
    25921 caaaccgaac tgccctatca agttcgtatc gagaactttg aggggcctct cgatcttctg
    25981 ctccacctca tcaagaagaa cgagatcaac atctatgaca ttcctgttca tctcatcgcc
    26041 cggcagtatc tcgagtacat cgaggcgatg aaagatctga atttaaccgt agccggcgag
    26101 tttctcgtta tggctgcgac gctgctccaa atcaaatcca aaatgctgct tccggtggat
    26161 gaagccgcgg aggatgagct tgaggggccg gatccgcgcg aagaattggt ccggcgtctg
    26221 cttgaataca agacgttcaa agaggcggcg cgccagttgg atacccagga acgcatgtgg
    26281 cgggatattt atacccgcac tccgatcgat tcggaggatc tggagaccga cgaacagtcg
    26341 cttgagaacg tcgggctgtt cgatctggtc gatgccttgc agggtgttct caaacgacat
    26401 ccgggcaagc agctcattga aattattccg gataacctga ccgtccggga tcggatgaat
    26461 gtaattttgg aagctctgga aggccaggag tcggtgagct tcgtgtcgct gttcgaggct
    26521 tcgtgccacc gcctggtcat tattgtgaca tttctcgcgt tgttggaact catccgtctc
    26581 aggactgccc gtgtgtttca ggcggagagt tttgggccga tcctggtctc gcgcgccttc
    26641 tcgttggtgc cggaccccgc ggaattggat gactcggaat ggaggtaagt atgagcgcaa
    26701 cggagctaca gggggcagag gttgagaatg taaatgtaat ggagacggtg tcggcggaac
    26761 aagataaccc tgctgtcggc catgagataa acggaaacgg caccgcatcg gccaacgctc
    26821 aggccgaggc attccaagga caggaactcg cggcggtcct cgaggcactt ctcttcgtgt
    26881 cggccgagcc ggttccgttg ccacggctgg cctctgtcat cgggacggtt tcaaaggcgg
    26941 aagtcgagca agcgctcaag atcctccagc agcaactcga gcaggaaggg cgaggcatcc
    27001 agctcgtgaa gctggcaggc gggtatcgcc tggtcacgaa acctgattac gccccctggt
    27061 tgaagcggtt ggataaggcc aaggccgcgc agaaactctc ccgctccgcc cttgaatcgc
    27121 tggcgatcat tgcgtataag caaccgctgg tgcgggccga aattgaagaa attcgcggtg
    27181 tggaaacctc gggcgtcatc cggacgttgt tggagcggaa actcgtgcga atcgtcggcc
    27241 gaaaggaagt gccgggtcgt ccgatcatgt acggcacgac gaagttcttc ctcgaacatt
    27301 tcgggcttca ggacctcagc caactgccgc cgcttcgcga attcaaggaa ctgggtgaag
    27361 ccgagcaggc gcttctgccg atcgaagaca ccaccgttgt tgatgttgag ccttcagaag
    27421 acatggtttc tcaggaaggt gcagcagagt cggcaattct ggattctgag gaaagagacg
    27481 cggtgctcgt gggaggagca tcgaccatgg aggcgcaact tcatgagcca ctccttgggg
    27541 agccagtcga gcaggcctag tctgaagagc tattttcccc gcgaaattca ctggatgaag
    27601 agtgcgacgt tggagcagat ctcgcgcaag caagtaggca agtaggattg tggagaggtt
    27661 tccgcttctc catgctatcc cctcagcttg atcgcccagg cccgttgact cctctctcac
    27721 ccctttgtta gactcctcaa tcccaaccta agttgttgaa agacgcaagg ttcttggttg
    27781 taacctgttc gagcaacacg aggcaccatc tatgatcaaa gcatggctga tggacgagat
    27841 gtttcggttc gatcgcgcat acctgacggt cgagggatcg cagggtgaat ggggcgccgg
    27901 cgattctatt gccgaggaag aagaactccc tcccgctcct gcgaatgttg cggccaagtg
    27961 tggaaatggc cggatcacta ttacatggga tccggttccg gatgccatgt actacaacct
    28021 ctatttccaa acaaccaaag gtgtccagat aaagttttca gaactgacca gaccgatcgc
    28081 gggcgaagat gatttcaaag ctgtcatcgg cgtgaagaaa gaaaaggcga cctgcctcga
    28141 aggcgttcag tctcctttcg tgcacgacga tctggccaac gggacttgtt accactatgt
    28201 cgtgacggtg gtgactccca agggggaaag tccggagtcg caggaagtta tggcgattcc
    28261 ctctccttat ctgttggcga tgagcctcgg gcaggagggc gtagacgacg gcgaattcag
    28321 ctcgccgacc ggcgtcgcct tggacaagga cgggaatatc tacgttgccg acaccgacaa
    28381 ccattcgatt cagaaattcg acaaaacggg aaagtttctt gcgcgctggg gcagcgagcc
    28441 ctcttcccaa gaaggcagtt tctactatcc ccgcggcttg gcgatcggag ctaacgatac
    28501 cctctatgtg gccgacagcg gcaacaaccg ggttcagaag ttcgatctcg aaggcaacgt
    28561 catgcaggcc tggggaaagt tcggtttcgc ctggcgtggc gccgatatgg gcaagttcga
    28621 tgtgccctgg ggattggcga cggaccagga aggcaacctg tatgtgtccg acaccagcaa
    28681 cgctcgaatt cagaagttca aggcggacgg cacaccgctt ttgaagtggg gccgggatgg
    28741 gagcttcgac ggggcgttct ttttcccgcg cggtgtggcg gtcgatttcg tcggcaacat
    28801 ttatgtggcc gatgaaagca acaaccgcat ccagaaattc gatacgcgcg gcagcttcct
    28861 gacgaagtgg ggccgtgaag gagccggtcc cgggcagttc aaatcgccct ggggcatcgc
    28921 gtgcgatgcg ttgggaaatg tctatgtcgt cgatactggc aaccaccgta ttcagaagtt
    28981 cgatgggaac ggcacgttcc tctgcgcctg gggtaatcgt ggaaagacgg aaggccagtt
    29041 gaacttccca tacgggatcg cagtcgacaa agaaggatgc gtctatgttg tcgacagcgg
    29101 caacaatcgc atactcaagt acgtgccgac ggacgaagaa ctgaatcgcg gcaaggatga
    29161 atcgcccaag tcactggagc cgaacaccac gccgccgccg catagcgtgg cggtgaaggc
    29221 cggggatacg gaagccttcc tcagctggat ggaagtccct ggcgcggtgt cctacaacct
    29281 ctatttcaac accacccctc agctgacgat acagagcgcg accaagatcg aaggggtgac
    29341 caatcccttt gcgcataccg gtctgaccaa cgacgttcct tatttctatg ccgtgacggc
    29401 ggtgtttgaa gacgggacgg aaagcgcgct ctcgaatgaa gtcaccgcga cgccggtcct
    29461 gatcgacatc acagcgccgc agaaccccta tgccgtgatg aatcacggcg ccttcatgac
    29521 gaactctccc gaagtggtcg tgacgatttc cgccaacgat atcgatacgg gtgtcggcgc
    29581 ctatttcatc tcggaagcgc cgatgacgcc aatggccggc acgccaggat gggtcgatgt
    29641 gacgccggca tacaagttcg gagcaacgat tccgttcatt ctctccccgg gcgacggaca
    29701 aaagtcgatc tatgtctggt tcaaggacgt cggcggcaac gtgtcgaccc cggcttccac
    29761 cagcattctg gtgaacacct ctggctatct ctgtgtttcg aaatggggcc ggacgggtcg
    29821 cggggcctca ctgctccatg gcggcgagtt catggcgccg ttgtacggcc tctgcgtcga
    29881 tcagcaaggc tcgttgtttg tcgtggataa cggcaacaac cgtgttcaga agttcgacaa
    29941 caccggcaat ttcatcatcc tctgggggaa ctttggttcc gccaatgcca acttccataa
    30001 tcccaccggc attgcctgcg acggcaaagg cgatgtgtgg gtggtcgata cgaacaatca
    30061 tcgcgtgcag aaattcgacg gcaaacttgg cggctacctc atgaagttcg ggtcccgcgg
    30121 gaatggcgaa ggccagttca actctccgtg gggtatcgcc gtggatcggg tgcggggcta
    30181 cgtgtatgtg gtcgacagcg cgaacttccg cgtgcagaaa ttcgacatga ccggtgagtt
    30241 catcatgtcc tggggcagct tcgggaacgg ggacggacag ttctatttcc cgcgcggcat
    30301 cgccatcgat cagacagacg gttcggtcta tgtcgtcgac atgggcaacc atcggattca
    30361 gaagttcgat acgagcacca acgtgttgcc gcagttgctg accaagtggg gcggcagttc
    30421 ggaaccgggc catgcgagca gcccgttggc tcaggaggcc ggtcagttgc gttcgccatg
    30481 gggaatcgcc gttgacagcg ccggcgacgt atttgtgacg gataccggga atcatcggat
    30541 tgaaaagttc gacaaggaag gcaacttcat cactcaatgg ggcggatacg gaaacggcga
    30601 cggacagttc aatttcccct atgggatttc ggtcgatgcg aagggaagcg tgttcgttgt
    30661 cgacagcgga aacacccgtg tccagcaatt catgccggct gatgaaggta gcgagcggct
    30721 tcaggaagag gccgaagcgt cggcggaaat tgaaaaggct caggggaccg cgagcacgta
    30781 agaggcgata ttatgcaggg ccattatgct ggataaagaa cccagattag gtctgactta
    30841 cgacgacgtc gtccttgtcc cggcgaaatc cgccatcctg cccagcgaag tcgagctctg
    30901 taccagcctg acacgaaaca ttcagatcaa tattcccgtc atcagttcgg ccatggatac
    30961 ggtgaccgaa tcccgcttgg cgattgccat ggcgcgtgaa ggcggactag ggattatcca
    31021 tcgtgtactc tcgccggcgg atcaagcggc agaagtggat cgggtgaaga aatctgaaag
    31081 cggaatgatt ctcgacccaa tcacgatctc gccggacgga acgattcgcg atgtctacga
    31141 gttgatgtcg aagtaccgca tttccggcat tcctgtcacg aagaacaaga agctggtcgg
    31201 gattctcacg aaccgtgatc tgcggttcga aacgcggatg gatctcaaag tgtcgcaggt
    31261 gatgaagcgc gacaagctca tcacggcccc tgaggggacc agtctggaga aggcccgcga
    31321 gatcttgcat gaacaccgga tcgaaaagct cccggtcgtc aacaagcagg gcgaactgaa
    31381 gggcctcatt accattaagg atatcgagaa gcggatcaag tatccgaatg cttgcaaaga
    31441 cgcgcatgga cggcttcgcg tgggcgcagc ggtcggtgtc ggccccgata cggaagatcg
    31501 cgtggtccag ttgaagaaag ccggcgtcga tctgattgtg gtcgatacgg ctcatggtca
    31561 ttcgcaggca gtgctggata cagtcaagat gatcaagaaa cagcacccga ccatggaatt
    31621 ggtcgcgggg aacatcgcca cggctgaggc ggccagagag ttgctcaaag ccggcgtcga
    31681 tgccgtgaag gtgggagtcg gtcccggctc gatctgcacg acacgaatcg tgtccggatc
    31741 cggcatgccg cagttgacgg cgatcgccga ttgcgcgaag gcgctagagg gcagcggcgt
    31801 accgatcatt gccgacggcg ggatcaagtt ttccggagac atcagtaagg ccttggctgc
    31861 cggcgcttcg tcggtgatgc tcggcggctt gctcgccggg acagaggaat ctcccggtga
    31921 gaccgtgctc taccaagcga gaacctataa ggtctatcgc gggatgggct cgatcggtgc
    31981 gatggagcgt ggagggggcg atcgctacgg gcagggcgga cgtccggcaa agaagctggt
    32041 gcctgaaggc atcgaagggc gtgtgcccta caaaggaccg ctgtcggcgg tcatctacca
    32101 gctcgtcggt ggcgtgaagt ccggcatggg ctattgcgga tgcaagacga ttcccgaact
    32161 ccagcagaaa gcgaccttta tacggcaaac cgtggcgggc cttcgtgaga gccatgtgca
    32221 cgacgtgatc attaccaaag aggcgccgaa ttaccggatg gattgggaat aggctccttg
    32281 aggcgtcagg cgtgagacgt taggcgatta gtttcgcaat ctgatcagat ctgtgactca
    32341 acactcctta cccctcaccc cttacgccta acgaattctg atggaactct ggcacaatag
    32401 aattctcgtc ctcgatttcg gatcgcagta cacgcagctg atcgcgcgcc gcattcgcga
    32461 agcgcaggtc tattcgcaga ttctcccctg caccgcgccg ctggcgacca ttctggccta
    32521 tcgaccgcag ggcatcatcc tgtccggcgg cccgtccagc gtctatgaga agaaggcgcc
    32581 gaccatctcc aaagagcttt tcgatcaggg cattccgatt ctcggcatct gctacggcat
    32641 gcaactggtc acccatctca gcggggggga tgtcgccaag tccaagcacc gcgagtacgg
    32701 ccgagccgat ctgacgatcg acgatgccag tgatctgttc aaaggaatcg gcacgggagg
    32761 atcgacggtc gtctggatgt cgcacgggga tcgcatcgag cggatgcctt cagggtttcg
    32821 gtccatcgca catacgagca attcgcccat cgcggcgatg aagcggaatg atcacaagcg
    32881 acggatctat tgcctgcagt tccatccgga agtcgcccac accgcggaag gcacgcagat
    32941 gctccgcaat ttcgtctacg atatctgtgg ctgtaagccg acctggacga tgcagtctta
    33001 tgtcgagtcg gccgtgctgc agatccgcga acaggtcggc aaggaacgtg tcatctgtgc
    33061 cttgagcggt ggcgtggatt cttccgtggc tgccgccttg acccataagg ccatcggtga
    33121 ccagttgaca tgcatttttg tcgacaacgg ggtacttcgg gccggtgaac gcgatcaggt
    33181 gcaaaagacg tttgcttcgc agatgcatct caatgtccgg attctcgacc ggaccacgca
    33241 gtttctggcc gggctcaaca atgtcacgga cccggagcgg aagaggaaaa ttatcggccg
    33301 gcagttcatc aagaatttcg aagaggagtc caggaaactg aagggcatca agtatcttgt
    33361 ccagggcacg ctctatcccg acgtgatcga gagcgtcagc ttcaaagggc cgtcggcgac
    33421 gatcaagacg catcacaatg tcggcggctt gccggctcga atgaaactga aattgatcga
    33481 gccgttgcgc gagctgttca aagacgaggt acgggtgctt gggaaggaat tgggattgcc
    33541 ggacgagatc atttggcggc aaccgtttcc cggtcccggc ctggccattc gcgtgctcgg
    33601 ctctatcaac agtgaacggc tggccatctt gcgggcggca gagacgatcg ttgatcagga
    33661 aattcgaggc gcgggactgt atcgtgagat ctggcaggcg tttgcggtcc tgttgccgat
    33721 tcgcaccgtc ggtgtcatgg gggatcaaag aacctacgag aacgtgatcg cgattcgcgc
    33781 ggtgaccagc ttggatggca tgactgccga ctgggcgaaa atcccgaacg aggtgttggg
    33841 ccggatgtcg aatcggatta tcaacgaagt gaagggcgtc aatcgagtgg tctacgacat
    33901 cagctccaag ccgccttcta cgattgaatg ggagtgagac gtcaatcgtg aatcgttaac
    33961 cgcaagagag agtctggcga ttcacgtttc acgcataacg tttaacgaga tggaaatcag
    34021 gcttcaaaaa ctcattgccg gagcgggcct ggcgtcgcgc cgcaaagcgg aagctctgat
    34081 tgcggccggc cgtgtgacgg taaatggcaa ggttgtgacg gaactgggta caaaggttga
    34141 tc
//
LOCUS       DATIFR010000080         4020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2540820, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000080 DATIFR010000000
VERSION     DATIFR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540820"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..472)
                     /locus_tag="VLD60_04410"
     CDS             complement(20..472)
                     /locus_tag="VLD60_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44230.1"
                     /translation="MAKLWVRKPGGAIRRKERMGVSPAGSLSLLWVSGSVTVLLTNSA
                     VANASGLETQPPGSAGKLSRPATVKLLVFPAASAAHRWLKSVVAISGFCPSGTILPLA
                     GEMGDWIEIVPCWPNCEEAEITLAVSGNRRLVVIEMFPPGPKTAFARI"
     gene            731..886
                     /locus_tag="VLD60_04415"
     CDS             731..886
                     /locus_tag="VLD60_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44231.1"
                     /translation="MLTTLTTTLSELNRLYGEYYDATSRSKDEAEKAWTAYIEYVKRY
                     KQERGII"
     gene            complement(959..1123)
                     /locus_tag="VLD60_04420"
     CDS             complement(959..1123)
                     /locus_tag="VLD60_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44232.1"
                     /translation="MKHVIYNTLIFGLLTAATAAVLFSIGVSLGHAQSSYLYSDGERY
                     PYGSDGITRF"
     gene            complement(1143..1283)
                     /locus_tag="VLD60_04425"
     CDS             complement(1143..1283)
                     /locus_tag="VLD60_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44233.1"
                     /translation="MRTVMATSIERFVSFALFTLGIAAAAVAAAALLLIIGDTLGHAW
                     GA"
     gene            1641..2498
                     /locus_tag="VLD60_04430"
     CDS             1641..2498
                     /locus_tag="VLD60_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSB44234.1"
                     /translation="METRNYVLWAVAILAVGMLSVAAGPSTESKDGQAPSFETIKGKD
                     GAPMVLIPAGPFTMGSNDGLPNERPEHTVTLEPYYIDQYEVTLALYRKFLESGKHDSP
                     PTWDDEAATTVGDRPAIGMKWADADAYCKWAGKRLPTEAEWEKAARGTDGRRYPWGHM
                     QPFVDIANYNRGLWVSEAITLVGVTSGLEGMSVRHGLKEGGKSPYGLSHMAGNAAEWV
                     ADWYDRDYYQKSSEKNPAGPPQGEKRVLRGGSWADLPAALRVTARFSAEPDFEDRTIG
                     FRCAMTPIK"
     gene            2602..3084
                     /locus_tag="VLD60_04435"
     CDS             2602..3084
                     /locus_tag="VLD60_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44235.1"
                     /translation="MSEKTVSALIVAVIALGLIWALRPVHQADPKPASAIVSLTPDMV
                     PLVTGDEPIVVIFTRAGCAVCHTIPGIPGADGKVGPPLLLGRTGPERLRDPTYRGRAK
                     TIHEYVMESVVEPAAFVVAGYPPNTMPTWYGAKLSALALEKMASYLEQQTSEAVSAGK
                     "
     gene            complement(3144..>4020)
                     /locus_tag="VLD60_04440"
     CDS             complement(3144..>4020)
                     /locus_tag="VLD60_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSB44236.1"
                     /translation="AEIQTVEELRLPAIMKDIAMTKRGLVLVVGATGSGKSTTLAAMI
                     DHRNSVHPGHIVTVEDPIEFVHNHKKSLITQREVGFDTLSFQNALKNALRQAPDVILI
                     GEVRDTETMEAAITFAETGHLCIGTLHSNNSNQAIERIMNFFPVERHAQIYLQLSLNL
                     RAIVSQRLVPSTDGKRVPALEIMMDTPRVKDLIKKAEVDTLKEAMEQGVDEGCQTFDY
                     VLLQLYKEGKISLEQALINADSANNLRLKIKLEGLKGDDAVNALLDKSATATGESPFK
                     IQGGAAGNVTPLRKR"
BASE COUNT          826 a         1070 c         1224 g          900 t
ORIGIN      
        1 ttgtgatctt gcagaacagt cagatcctgg caaacgccgt cttcgggccg ggtggaaaca
       61 tctcaatcac gaccaatctt ctgttgccgg atacggctag cgtgatctcg gcctcctcgc
      121 aattcggcca gcaaggcact atctcgatcc aatcgcccat ttctcctgcc agcggcaaga
      181 tcgttccgct cgggcagaag ccgctgatcg caaccacact cttgagccaa cggtgcgcag
      241 cactcgccgc cgggaacacc agcagcttta ctgtcgccgg gcgagacagt ttgccggccg
      301 aaccaggtgg ctgggtttcg agtccgctgg cgttggccac cgctgaattg gtgagcagta
      361 ccgtcactga gccggacacc cacaggagac tgagtgaccc agcaggggag acgcccatcc
      421 tttccttgcg gcggatcgcc cccccgggtt tcctgaccca gagcttcgcc acggactcgt
      481 ctggaggctg cacgtcataa cgaggatggc cgtgcggcag acgatggctt agaacactgt
      541 agacgaagct gaatggcaga ccgtggcgac cggctaacag cgaggaccgt gccgacgggg
      601 gaaggagtcg cagcgccgtt cgcgggttcc gtcggacaca gggaaacgca cacgtcaggg
      661 ttcctctttt gccaagtggt tgcttgggat cagtgtccag taggatcgtt ggcccgcgaa
      721 aagtaagtac atgctaacga ctctcaccac tacgttaagt gaactcaatc gcctctacgg
      781 ggaatactac gacgccacct ctcgctccaa ggacgaagcg gagaaagcct ggactgccta
      841 catcgaatac gtaaagcggt acaagcagga gcgcgggata atctagtagc gcaaagcggc
      901 ctaaggacgc atacgcctcg gcttgatgcc tgacattttg atttcgattg gtgcggaatc
      961 agaagcgggt tattccgtcc gatccgtaag gatatcgctc accgtcactg tagaggtacg
     1021 acgactgagc atgtccgaga gaaacaccaa tactgaagag gactgcggcc gtcgcagcgg
     1081 tgagtaaccc gaagatgagc gtgttgtaga tcacgtgttt catggcggct gttcgtttgg
     1141 tgttatgccc cccacgcgtg acccaaggtg tccccgatga taagcaggag ggcagcggcg
     1201 gcgacggctg cggcggcaat gcccaacgtg aataaggcga agcttacgaa tcgttcgatg
     1261 ctggtagcca ttacagttcg catggtaccg gggttccttt ccttgttgtt ttatgctcca
     1321 ctctgcggag cacaaacaat taccctttag ggttggaagg aaccgaagta catcgaacga
     1381 gggggttgtc aaaactaggc ggggaaagct acagtttcgc gtatgtcaac agctacggtt
     1441 tcttgtgaag tggctcccag cgaggatttc aagtagtcct gtctgagtct cccgttgggg
     1501 acaccttacc ttgtcatgcc tgtcatcttc tttggcgtcc attcgctctc tcactccatt
     1561 gagtttgctc cttaagctgg gtagactgtt tcccgagttg cgggatggtc tgccgctgag
     1621 atgaaggagg ggatggtcgt atggaaactc ggaactatgt cctttgggct gtggccattc
     1681 tagctgtggg aatgctgtcg gttgccgcgg ggccatcaac agaatcaaaa gacgggcagg
     1741 ctccttcttt cgaaaccatc aaaggcaagg acggcgctcc gatggtcctg attcctgccg
     1801 gtccctttac aatggggagt aacgatgggt taccgaacga gcgtcctgaa catactgtca
     1861 cgcttgagcc ctattacatc gatcagtatg aagtcacgct ggcgctttat agaaagtttc
     1921 tcgaatcagg gaagcatgat tcgccgccta cctgggatga tgaagctgcg acgacggttg
     1981 gggatcgccc tgcgatcggc atgaaatggg ctgacgcaga tgcctactgc aaatgggcgg
     2041 gaaaacggct tccgactgaa gcagagtggg aaaaggcggc gcggggaacc gatggtcgac
     2101 gctatccctg gggccatatg cagcccttcg tcgacattgc caactacaat aggggactct
     2161 gggtctccga ggcgattacg ttggtcggag tcaccagcgg actcgaagga atgagcgtcc
     2221 gccatgggct gaaggaaggg ggaaagagcc cctatggttt gagccacatg gctgggaatg
     2281 ccgccgaatg ggtcgcggat tggtacgatc gtgactatta tcagaagagc tccgagaaaa
     2341 accccgcagg gccgccacag ggtgaaaagc gggtcttgcg cggcggttcg tgggcggacc
     2401 ttccggccgc gttgcgtgtc acagctcgat tttccgcaga gccagatttt gaggacagga
     2461 caatcggttt ccgctgcgcg atgacgccga tcaagtaagc cgctgcgccg agcaggtgcg
     2521 atcgcatcgt gcttggcgca cgaatcggtt tttctcatac tgccgctacg acccctcgta
     2581 tcatgcccgg attttcttct catgtctgaa aaaaccgtga gcgcgctcat tgtcgctgtc
     2641 atcgccttgg gcttgatttg ggcattacga cccgtgcatc aggcagatcc caaaccggct
     2701 tccgcgatcg tgtcgctgac gccagacatg gttccactgg tcacaggaga tgaaccaatt
     2761 gtcgtgatat tcacgcgtgc cggttgcgcg gtctgtcaca cgatcccggg tattccaggg
     2821 gctgatggca aggtcggtcc cccgcttctg cttggaagga cggggccgga acgattgcgg
     2881 gatccgacct ataggggccg ggcgaaaacg attcacgaat atgtgatgga atcggtggtc
     2941 gagcctgctg cgttcgttgt ggcgggctac ccccccaaca cgatgcccac gtggtatggg
     3001 gcaaaattga gcgcgctggc tttggagaaa atggcgtcgt atcttgagca gcaaacgtcc
     3061 gaagcggtga gcgcggggaa gtagcggcgc ccgtcagttc acgacgagcg cgcattcgtg
     3121 aatcagtttg ataagggaag acgctatcgc ttgcgcaagg gggtgacgtt gccggcggcg
     3181 ccaccttgaa tcttgaaggg gctttcgcct gtggccgtgg cgctcttatc gagcagcgca
     3241 ttgaccgcat cgtctccttt gaggccttcc agcttgattt tcaagcgcag gttatttgcg
     3301 ctgtcggcgt tgatcagggc ctgttcaagc gagatctttc cttccttata gagttgcagg
     3361 agcacatagt cgaaggtttg gcaaccttcg tcgactcctt gctccatcgc ttccttcaac
     3421 gtatcgactt cggccttctt gatcaggtcc ttcacgcgag gcgtatccat catgatctcc
     3481 agcgccggca cccgtttccc gtccgtagag ggaacgaggc gctgggagac aattgcgcgc
     3541 agattcagcg agagttggag atagatttgg gcatgtcgtt ccaccgggaa gaagttcatg
     3601 atgcgttcga tggcttggtt cgagttattg gagtgtaacg ttccgatgca gaggtgaccg
     3661 gtttccgcga aggtgatggc ggcttccatt gtttccgtgt ctctgacttc gccgatgaga
     3721 atcacatcgg gcgcctggcg tagcgcgttc ttgagcgcgt tttgaaacga gagtgtgtcg
     3781 aaccccacct ctcgttgcgt gatcagtgac tttttgtgat tgtggacgaa ctcgatcgga
     3841 tcttccacgg ttacgatgtg gccggggtgg acggaattgc ggtgatcgat catcgccgcg
     3901 agcgtggtcg atttccctga gccggtggcg ccgacgacga gaaccagccc gcgcttcgtc
     3961 atcgcaatgt ctttcatgat ggcagggagt cggagctctt ccactgtctg gatttcggct
//
LOCUS       DATIFR010000081         9303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1199989, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000081 DATIFR010000000
VERSION     DATIFR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199989"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1797)
                     /locus_tag="VLD60_04445"
     CDS             complement(67..1797)
                     /locus_tag="VLD60_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44237.1"
                     /translation="MSTFSRFLPFLKPYLSRMVLAGMLVMAVAAINLALLRLAGMLWD
                     VITVQHDAPRMTEMIALFLGLVILQGLCSMGHSYLTAWVSQHIVADFRTHLFAHLQTL
                     SVSFFARRRTGELLSRLMNDVTVIQSVVTETPIDTAKQLVTFVGGIAFLLAMNWRLCL
                     LILLLLPLLVVVAKVFGRKLKSLSTSIQDQTAALSTLIEEVISGIRIVKSFVQTKREE
                     TRFTAQVGQNLSLTMRRAGIMAVFIPVISLLTFSAAAAVLWYGGRQVIDGTVSPGDLF
                     AFVLFAGILIGPFSSAARVFAQVREAQGATERVFEILDQQPEVSDSPTADVLANVSGH
                     IRVEHMSFAYDARQPVLTEVSFEAQPGELVAIVGPTGAGKTTAMNLIHRFYDPTEGRI
                     TIDGQDLRQVTLESWYRQIALVPQETILFGGTILDNIRYGNREASEEMVEAASRAAHA
                     HDFIMSFPDQYHTVVGEKGINVSGGQRQRIAIARAILKNPRILLLDEATSALDSESER
                     LVQEALEQLMKGRTTFVIAHRLTTIQRADCILVLNKGRLVEAGTHSELMDRQGLYHYL
                     YTLRLTELPS"
     gene            complement(1878..2096)
                     /locus_tag="VLD60_04450"
     CDS             complement(1878..2096)
                     /locus_tag="VLD60_04450"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44238.1"
                     /translation="MPEGKKVRIRVRTVNCVYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVIINEKDRSDFVAVNKNLIESIAQI"
     gene            complement(2126..2221)
                     /locus_tag="VLD60_04455"
     CDS             complement(2126..2221)
                     /locus_tag="VLD60_04455"
                     /inference="COORDINATES: protein motif:HMM:NF019820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HSB44239.1"
                     /translation="MASVLKKRRKKMRKHKYKKLRRRQKFLRRKS"
     gene            2387..2899
                     /locus_tag="VLD60_04460"
     CDS             2387..2899
                     /locus_tag="VLD60_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44240.1"
                     /translation="MKISFRSRAFVLTLAAAALFFVGAGCSAKTIQYPEDHERYLRID
                     QAVESLRYSYERKNASKMADLMLPTEQIDRLQRDVDNDFESFYSITLEFAIERIMIEG
                     DDIDVYVHWQGVWKRDADDPGFRQRGHSRLQWVGTKVVLLRGIQGDAPFGAKARQALA
                     SPATTPAVKK"
     gene            2903..4546
                     /gene="nadB"
                     /locus_tag="VLD60_04465"
     CDS             2903..4546
                     /gene="nadB"
                     /locus_tag="VLD60_04465"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HSB44241.1"
                     /translation="MPARYSHSDHQADFLVIGSGVAGLRAALELSRSGRVLVLTKGHP
                     LQSSSIHAQGGVAVAMSEEDDVAIHLTDTLKAGHGLCRKEAVRVLVGEGPQRIQELIK
                     WGAKFDKTGGKFAFAREAAHSRSRILRARGDATGNEMVRVLIAEAMRQKRIQRLDYHF
                     TVDLIIEAGRCCGALVLDEHAGRQFLLPTRAVVLTTGGAGQVYARTTNPANATGDGMA
                     MALRAGAVLQDMEFVQFHPTALYLPSSPPFLLSEAMRGEGAQLRNNKGELFMQRYHPM
                     SALAPRDIVSRAIWAEMAATKARHVYLDVTHLGADFIKRRFPTIYATCLRYDIDITEE
                     WIPVSPSAHYMMGGVWTDLNGATTVPGLFAAGEVACSGVHGANRLASNSLLEGLVFGA
                     RAATAAVAYAGRYDIPSLAAHEAALKTEHVGSIEDVEKLRSSLRRTMWGQVGVIRSGE
                     SLIRACAQLSRWAQLVAQPFSNRAALEVKNMVQVAQCIAEAALWRENSVGAHYRSDFP
                     QAKRPGWQQHSRLSMGEELSEKKSRKSRGLLLALRGRAG"
     gene            complement(4482..5183)
                     /locus_tag="VLD60_04470"
     CDS             complement(4482..5183)
                     /locus_tag="VLD60_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB44242.1"
                     /translation="MAMKSKQSRQKAPFRMAAIAIFGFSLLGITSPAASQAEIYQYIG
                     ANGTISLTNVPSDARYRKIELESARFHAILSERELEPVIKRHSSQQQLHPALIRAVIK
                     AESNFDPRAVSRAGAIGLMQLMPQTAARMDVHDMYDPDDNVAGGTKYLRQLLDRFHGN
                     LPLALAAYNAGENIVDRYQALPPIDETRQYVRKVLRYYRTFLVRDGVITERPVSRHAP
                     VAPAEDPAISGTSFR"
     gene            complement(5300..6169)
                     /locus_tag="VLD60_04475"
     CDS             complement(5300..6169)
                     /locus_tag="VLD60_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSB44243.1"
                     /translation="MWIYLNNRFVKETEALVSVFDHGFLYGDGVYETIRSYGNRIFMR
                     DQHLARLRRSANAIGLTIPDLDWPALLHEAMNRNEVGNDRTDAYMRITISRGVGDIGL
                     DPALCPTPTIVIITKPLRPPSSQLYQEGVSLITARTKRNLPSALDPQIKATNFLNNIL
                     AKREAIAAGAFDSLLLNWESHITECTVSNVFFVKTGQLCTPSLDCGILDGITRTIILT
                     LAREEQISVKEGRFGIDALRQADECFLSNTTMEIMPVTILDRQPIGLGRPGPLTQRLH
                     QLFVIQRRRFVEP"
     gene            complement(6172..7632)
                     /locus_tag="VLD60_04480"
     CDS             complement(6172..7632)
                     /locus_tag="VLD60_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSB44244.1"
                     /translation="MARFSQPTFLNGPPEPLVLTLDGWNESPLELYGKIASPSRPSFL
                     LESGKRLTDEPSYSFFGSDPIAILAGTTAVANGTAEEPFKQLARIFDGSNFIRTPDFP
                     PFFGGAVGYFSYDFVRRYETLPSNATNDLDIPDLQFCLFDLITAIDHRTNRVQIIFCP
                     SMERFLGEARDKLYREGQDRLAECEARLRDGTVSEAAASPLESLSFTPNQTKEAYLNR
                     VRRCQEYIAAGDIYQANLSHRFTVETPPWSRRGSDRLRYEQALYRQLRAVNPSPFSGL
                     LHFDDVSLISCSPERLVRLQGRHVETRPIAGTRPRGCNAMDDQRLVGELLANEKERAE
                     HLMLVDLERNDLGRVCEFGSVHVDEFMAIEQYSHVSHIVSNISGTLRKDATPLDLIRA
                     TFPGGTITGVPKIRCMEIIDEVEPVRRGPYTGSFGYIGWNGDLDLNIIIRTLVLCGAK
                     GYLQVGAGIVADSDPTKEYEETLHKAQAFFSSLQRT"
     gene            7810..8055
                     /locus_tag="VLD60_04485"
     CDS             7810..8055
                     /locus_tag="VLD60_04485"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSB44245.1"
                     /translation="MPPTQDPFYAGLGQAVRIGTELLAALIVGGGLGWAADTYLFGTT
                     PWGMVVGLVLGAAAGVRNAYRAAQRWQDSSDNSDKQK"
     gene            8067..8819
                     /locus_tag="VLD60_04490"
     CDS             8067..8819
                     /locus_tag="VLD60_04490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSB44246.1"
                     /translation="MEESPLHPFELHNFIPLSLGGLDISINKAVIMMWVVVAVVTFLM
                     VKAALARQLIPGKVQSLAEILVDFIRSMILDTMGKEGIKFFPLIATLFLFILFCNWLG
                     LIPGTYTVTSQIIVTGVFAGSVYGLSIILGFVLHGMKFLGILVPPGTPGWLLPLVIPI
                     EIVSQLARPVALAVRLFANMTAGHTILAVLFGMVVSLPFYVSWLPFAATLFVNTLEVC
                     LIAPIQAYIFTILTCVYLGDAVTLHGHSEQAH"
     gene            8876..9106
                     /gene="atpE"
                     /locus_tag="VLD60_04495"
     CDS             8876..9106
                     /gene="atpE"
                     /locus_tag="VLD60_04495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSB44247.1"
                     /translation="MDSAAAALLGMGMAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
BASE COUNT         1968 a         2384 c         2866 g         2085 t
ORIGIN      
        1 ccagggtcgg tcaaagcaag ctggtcttac agagatgcgc aggagattat gggcgccggg
       61 cgagagttac gagggcagct ccgtcagccg gagggtatag agatagtggt agagcccctg
      121 acggtccatg agttcgctgt gcgtgccggc ttccacaagg cggcctttat tcagaacgag
      181 gatgcaatcg gcccgctgga tggtggtgag gcgatgggca atcacgaacg tggttcggcc
      241 tttcatcaat tgttccaacg cttcctgcac cagccgctcc gattcgctgt cgagggctga
      301 agtggcctca tccaacaaga gaatccgcgg attcttgaga atcgcacgag cgatggcgat
      361 gcgttgccgc tgcccgcctg atacgttgat ccctttttct cccacgacgg tgtgatattg
      421 gtcggggaag ctcatgatga aatcgtgggc gtgggccgca cggctggctg cttccaccat
      481 ttcttcgctg gcttcccggt taccgtagcg gatattgtcg agaatggttc cgccaaacag
      541 aatcgtttct tgcggcacga gtgcaatctg ccgataccag ctttccaggg tgacctggcg
      601 gagatcctgg ccgtcgatcg tgattcgtcc ctcggtcgga tcgtaaaacc ggtggatcag
      661 attcatcgcg gtcgtttttc ctgcgcccgt agggccgacg atcgcgacga gttcgcccgg
      721 ttgggcttcg aaggagactt cggttagcac cggctggcgg gcatcgtaag cgaaactcat
      781 gtgctcgaca cggatatgtc ctgacacgtt cgccaagacg tcggcggtgg gggagtcgct
      841 gacttccggc tgctggtcca aaatctcgaa tacccgctcg gtggcgccct gcgcttctct
      901 gacttgggcg aagacgcggg cggcggaact gaacggaccg atcagaatgc cggcgaagag
      961 cacgaaggca aagagatcgc ccggtgaaac cgtgccgtca atgacctggc ggccgccgta
     1021 ccacaagacg gcggccgcgg cggaaaaggt caggaggctg atgaccggaa tgaagacggc
     1081 catgatgccc gcgcgccgca tcgtgagaga cagattttgc ccgacctgtg cggtgaatct
     1141 cgtctcttcg cgcttggttt gaacgaagga tttcacgatg cggatgccgg aaatcacctc
     1201 ttcgatcaag gtgctgagag cggcggtctg gtcttggatc gaggtcgaaa gcgatttcaa
     1261 tttgcgcccg aaaacttttg cgacgacgac gagcaaagga agcaggagca ggatcaagag
     1321 gcacagtcgc caattcatcg ctagcaagaa ggcgatgccg ccgacgaacg tcacgagttg
     1381 tttggcggtg tcgatgggcg tctcggtcac caccgactga atgaccgtga cgtcgttcat
     1441 caggcgcgac aggagttcac cggttctccg gcgggcgaag aagctgacgg ataaggtctg
     1501 caaatgcgca aacagatggg tgcggaaatc ggcgacgata tgctgagaaa cccaagcggt
     1561 cagatagctg tggcccatgg agcagaggcc ttgcagaatc acgagcccga ggaacagcgc
     1621 gatcatttcg gtcatgcgcg gagcgtcatg ttggacggtg atgacgtccc agagcatgcc
     1681 ggccaatcgc aacagcgcca gattgatggc ggccacggcc atgaccaaca ttcccgccag
     1741 gaccatgcga gagaggtatg gtttgagaaa cggaagaaag cgcgagaacg tcgacatcgt
     1801 ggggagcgat cttagaagag atgaccgatg aaaaccaagc gagaaacacg agccagcacg
     1861 agggagaaaa acggaagtta aatctgggcg atggactcga tgaggttctt attgacggcc
     1921 acgaaatccg accgatcctt ttcgttgatg atcacgtcgg tgaggctgat aaacagccgc
     1981 tgctcttcgt tgatcgcgtc ggaaacgcga ttgcgcatgg gaggaatgag gaaatcgccc
     2041 acgtagacgc agttgacagt gcggacgcga atgcgaacct tcttcccttc gggcacggat
     2101 ccctcctccc ctgtgcgggg tgagattaac ttttacgacg aaggaatttt tgacgccggc
     2161 gcagcttctt gtacttgtgc ttgcgcatct tcttccggcg ttttttcagc acgctagcca
     2221 tacgatctct ctcctacggg cgtgagtcta gaagttttcc ggccaaattg caagtaacag
     2281 aaggaattgt aagcagtctt gagcatcgca tcgtacgata gatgcgtgtg gaatagcgac
     2341 gacgagcagc ttgacctctc ccgcggccca ttcctatact gcaaacatga aaatttcatt
     2401 tcgcagtcgt gcgtttgtcc tgacgcttgc cgccgcggcg ttgttcttcg ttggtgccgg
     2461 ctgcagtgcg aagacgattc aataccccga agatcatgag cggtatctcc gcatcgacca
     2521 ggcggtggaa tcgttacgct attcctatga aagaaagaat gcgtccaaga tggccgacct
     2581 gatgcttcca acggagcaga ttgatcggct tcagcgtgat gtggacaacg atttcgagtc
     2641 gttttactcc ataacgttgg agtttgccat cgagcgcatc atgatcgaag gcgacgacat
     2701 cgatgtgtat gtccattggc aaggggtgtg gaaacgggat gccgatgatc ccgggttccg
     2761 ccaacggggc cactcacgcc tgcaatgggt ggggacaaag gtcgttctgc ttcgagggat
     2821 ccagggggat gcgccgtttg gggcgaaggc gcgacaagcg ctggcttcgc ctgccacgac
     2881 tccagcggtg aagaagtaat tcatgccagc ccgctattct cactccgacc atcaggctga
     2941 ttttctggtc attggcagcg gtgtcgcagg cctacgcgcc gcgcttgaac tcagccgttc
     3001 cgggcgtgtg ttggtgctga cgaaggggca tccgcttcag agcagctcga ttcatgcgca
     3061 gggcggcgtc gcggttgcga tgagtgaaga agacgatgtg gccatccacc tgaccgatac
     3121 gctcaaggcg ggtcacggtc tgtgccggaa ggaagcggtg cgcgtgctgg ttggagaggg
     3181 tccacagcgg attcaggagt tgatcaaatg gggagccaag ttcgacaaga ccggtggaaa
     3241 atttgcgttt gcgcgcgaag ccgcgcacag ccgcagccgc attctccggg cacgtggcga
     3301 tgcgacgggc aatgaaatgg tgcgcgtgtt gatcgccgaa gccatgcggc agaagcgcat
     3361 ccaacggttg gattatcact tcaccgtgga tctgatcatc gaagccggcc gttgttgcgg
     3421 cgcactcgtg ctcgatgagc atgcaggccg gcagtttctg ttgcctaccc gcgcggtggt
     3481 attgacaacc ggcggggccg gtcaggtgta tgctcgaacg accaacccgg cgaatgcgac
     3541 cggtgacggc atggcgatgg cgcttcgcgc cggcgcagtg ctccaggaca tggaattcgt
     3601 acagttccat cccaccgctt tgtatcttcc gtcgagcccg ccgtttctgc tttcggaggc
     3661 gatgcggggc gagggggctc agttgcgcaa caacaagggc gaactcttca tgcagcgcta
     3721 ccatccaatg agcgcgctgg cgcctcgtga cattgtgtcc cgggcgattt gggccgagat
     3781 ggccgcgacg aaggcccggc atgtgtatct cgacgtcact cacttgggcg cggacttcat
     3841 caaacggcgc tttccaacca tctacgcgac gtgtttgcgc tacgacatcg atatcacgga
     3901 ggaatggatt ccggtctctc ccagtgccca ttacatgatg ggcggggtct ggaccgatct
     3961 gaatggggcg accacagttc ccggtttgtt tgctgcagga gaagtggcgt gcagcggcgt
     4021 gcacggcgcg aatcgtctgg cgagcaattc gttactggag ggcttggtgt tcggtgcgcg
     4081 ggcggcgacg gcggcggtgg cctatgcggg gcgctatgac attccttcgc tggcagccca
     4141 cgaggcagca ttgaagaccg agcatgtcgg ttccatcgag gatgttgaga agcttcgcag
     4201 ctccctccgg agaacaatgt gggggcaagt cggcgtgatt cgatcaggcg agtcgctcat
     4261 cagagcctgc gcgcagcttt cccgctgggc gcagcttgtg gcccagccgt tttccaatcg
     4321 ggcggcattg gaagtgaaaa acatggtgca ggtcgcgcaa tgcattgcgg aagcggcatt
     4381 gtggcgggag aacagtgtcg gggcccatta tcggtccgat ttcccgcagg ctaagcgccc
     4441 gggctggcag cagcacagtc gattatcgat gggggaagaa ctcagcgaaa agaagtcccg
     4501 gaaatcgcgg ggtcttctgc tggcgctacg ggggcgtgcc ggctgacggg tcgttccgta
     4561 atgactccgt cgcgtaccaa gaatgtgcgg tagtaacgta gaactttccg aacatactgc
     4621 ctcgtttcgt cgatcggagg aagggcttga tagcggtcga cgatattctc accggcatta
     4681 taggccgcca gtgccagcgg taaatttccg tggaagcgat cgaggagctg ccgcaggtac
     4741 tttgttcctc ctgccacgtt gtcgtccgga tcatacatgt cgtgcacgtc cattcgcgcc
     4801 gcggtttgcg gcatcaactg cataagaccg atcgctcctg cgcgagagac agcccgggga
     4861 tcaaagttcg attcggcttt gatcaccgcc cggatcaatg ccgggtgcag ttgctgttgg
     4921 gatgagtggc gcttgataac cggctccaat tcgcgttccg acaagatggc gtgaaagcgg
     4981 gccgattcca gttcgatttt tcgataacgg gcatccgagg ggacattggt gagtgagatg
     5041 gtaccgttgg ctcctatata ttggtagatc tccgcctgag aggctgcggg ggaggtaatc
     5101 cccaggaggc tgaatccaaa aatggcgatg gcagccattc ggaagggggc cttctgccga
     5161 gattgttttg atttcatggc catatgcatg ttcccaacga taccagccta gggtggcatg
     5221 tcaaggaaat gcagttcctg tgccggagta ttccggagaa agtctcgcaa gaacaagagg
     5281 atcgagcgag gcattgagct cagggttcga caaatctacg gcgctggatg acaaaaagtt
     5341 ggtggagtcg ctgggttaag ggacctgggc gccccaggcc gatcggttgg cggtctaata
     5401 tcgtgacggg catgatttcc atagtggtgt tgctgaggaa acattcgtcc gcctggcgga
     5461 gagcatcgat gccgaatcgg ccttctttta cggagatctg ctcctcgcga gccaaggtca
     5521 gaatgatggt gcgagtgatt ccgtcgagaa tgccgcagtc gagggagggg gtacagagct
     5581 ggcctgtttt aacgaaaaat acgttgctga cggtacattc ggtgatgtgc gattcccaat
     5641 tcagcaacaa gctgtcgaag gctccggcag cgatggcttc acgcttggct aaaatattgt
     5701 tgaggaagtt ggtggctttg atttggggat ccagtgcact cgggagattg cgtttggtcc
     5761 tggccgtaat gaggctgaca ccttcttggt aaagctgcga tgaaggcggc ctgagcggct
     5821 tggttatgat cacgatggtc ggagtggggc agagagcagg atcgaggccg atgtcgccga
     5881 cgcctcgtga aatcgtaatt ctcatgtaag cgtcggtgcg gtcgtttccc acttcgttgc
     5941 ggttcatcgc ctcgtgcaag agcgccggcc agtcgaggtc gggaatcgtc agaccgatcg
     6001 cgttcgcgga gcgtcgaagt ctggccaggt gctggtcccg catgaagatc cggttcccat
     6061 aggaacgaat ggtttcgtag acgccgtcgc catacagaaa cccgtgatcg aagaccgaga
     6121 cgagcgcctc cgtttctttg acaaaccggt tgttgagata aatccacatc gtcaggtccg
     6181 ttggagtgaa ctgaaaaatg cctgggcttt gtgcagcgtc tcctcatatt ctttggtcgg
     6241 gtcagaatcg gcgacaattc ctgctccgac ctgcaagtat ccctttgccc cgcacaggac
     6301 gagcgttcga atgatgatgt tcaggtccag atccccgttc catccgatat acccgaagga
     6361 tccggtgtaa ggtcctcgtc ggaccggctc cacttcatcg atgatttcca tgcaacggat
     6421 tttagggacc cccgtgatgg tgccgcctgg aaaggtggcc ctaatcaagt cgagtggggt
     6481 cgcatccttg cggagcgtgc cgctgatatt ggagacgatg tgactgacat gcgaatactg
     6541 ttcgatggcc ataaactcgt cgacatggac gctcccgaat tcacagaccc gtccgaggtc
     6601 gttgcgttcg agatcgacga gcatcaggtg ctcggcgcgt tccttttcat tggccagcag
     6661 ctcgccgaca agccgctggt catccattgc attgcagccg cgcggtctgg tgccggcaat
     6721 cgggcgtgtc tcgacatggc gtccctggag gcgtaccaat cgttccggtg agcaactgat
     6781 aagtgacaca tcgtcgaagt gcaggagtcc ggagaaggga gaaggattca ccgcacgcag
     6841 ctgccggtag agtgcctgct cgtatcgaag ccgatctgat ccgcgacgag accatggggg
     6901 tgtctcaact gtgaatcgat gcgaaagatt ggcctgataa atatcaccag cggcgatgta
     6961 ctcttgacat cgacggacac gattcaaata ggcttccttc gtttgattcg gcgtgaacga
     7021 cagcgactcg aggggtgaag ccgctgcttc ggagactgtg ccgtcacgaa gcctggcttc
     7081 acactccgcc aatcggtctt gtccctctcg atagagcttg tcacgtgcct cccccagaaa
     7141 tcgctccatt gagggacaaa aaataatctg aacgcgattg gttcggtgat cgatagccgt
     7201 gatcagatca aagaggcaaa attgcaggtc tggaatatcg aggtcattcg ttgcattgga
     7261 cggcagcgtc tcgtagcgac ggacgaaatc gtagctgaag tagccgactg ctccaccgaa
     7321 gaacggaggg aagtccggtg tccgtataaa gttggaccca tcaaagattc gagcgagctg
     7381 cttgaatggc tcctctgctg ttccgttagc gaccgcggtg gtgcccgcaa ggatggcaat
     7441 ggggtcactg ccgaaaaatg agtatgaagg ctcgtctgtg aggcgcttcc cactttcaag
     7501 cagaaaggat ggacgtgaag gagaagctat tttcccgtag agttcgagcg ggctctcatt
     7561 ccaaccatcc agggtaagga caagcggctc gggggggcca ttgaggaatg tcggttgaga
     7621 gaatcgggcc atgggacagt atctgctcgt gatccacaca agggaggttc actatactcc
     7681 aggtgtcggt cgtttcgcag cagctgcaac ttgacaaatt cacgaggatt ctgcttgaat
     7741 tgcgcgcccg ccattgggag agtccttact cagcaactgc atgagtactc gcctgcacgt
     7801 gggtaacaca tgccccctac acaggatccg ttttatgcgg ggctcggcca ggccgtccga
     7861 atcgggacgg aactgttggc tgcgctgatc gtcggaggag gactggggtg ggcggcagat
     7921 acctatctct ttgggacaac tccctgggga atggtggtgg gtttggtgct cggcgccgcg
     7981 gccggtgttc gaaatgccta tcgagccgct cagcgatggc aagattcatc agataattct
     8041 gataaacaaa aataggtcaa agggcaatgg aagagagtcc gctacacccg tttgagctgc
     8101 acaactttat ccctctcagc ctcggtgggt tggatatttc catcaataaa gctgtgatca
     8161 tgatgtgggt agtggttgcc gtggtcacat ttcttatggt caaggcggcg ttggcacggc
     8221 agctcattcc cggcaaggtg cagagcctgg cagagatttt ggtagatttc atacggagca
     8281 tgatactcga cacgatgggc aaagaaggga ttaagttttt tcccctcatt gccactctct
     8341 ttctattcat tttgttttgc aattggctag gccttatacc cgggacttac acggttacca
     8401 gccagattat cgtaactggc gtgttcgccg gatcggtata cggactcagt attatcctag
     8461 ggtttgtgct tcacgggatg aagtttctcg gaatactggt tcctcctgga acacctgggt
     8521 ggttgctccc tctagtaata ccgattgaaa tcgtcagtca acttgcacgg ccggtcgcgc
     8581 tcgcggttcg gttgttcgcc aacatgactg ctggacacac gattctcgcc gtgctgtttg
     8641 gtatggtggt ttcgttgccc ttctatgtaa gctggcttcc gtttgcagcc acgctctttg
     8701 tgaataccct ggaagtgtgt ctgatcgcac cgattcaagc ctacattttt acgattctaa
     8761 cttgtgtcta tcttggggat gcagtgacat tacatggcca tagtgagcag gcgcactaag
     8821 cacactaata aaatgggtgt gagtcagcaa tagataagaa ggaggagcac atcaaatgga
     8881 ttcagcagca gcagcattgt taggtatggg catggccgca gcaggatttg ccggggccgg
     8941 cgtagggatt ggatatattt tcggcaaaat gatcgaagct gtggcacgtc agccggaagc
     9001 agaaggccgc gtcgggaagt acatgtggat cgggtttgcc ttggttgaag cgatcgccct
     9061 gtacggcctg gtcatcgcat tcattatcat gggcctccgc aaataggagc aggcccaggt
     9121 ttcggtcaag agcgcgaagg gttgtatttc ttcctccgcc tcaaccttaa ccttcctgat
     9181 gtgaactatg ccgcagtttg attcacactt tttttcgtcg ttgatcttct gggaggtcat
     9241 ctccttcgcc attctgttct ttgcgctcta caagttcgcc ttccctggca tcttgagtat
     9301 gct
//
LOCUS       DATIFR010000082        29948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1008864, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000082 DATIFR010000000
VERSION     DATIFR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 29948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29948
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008864"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VLD60_04500"
     CDS             complement(<1..1078)
                     /locus_tag="VLD60_04500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSB44248.1"
                     /translation="MAWIRHVDAVRWLVLASMIVGAGSGCAVNPVSGRPELTLISAKQ
                     EQELGAEEAKKVEAGMGFADESGFTPYLDQLGQRLAEQSPRKNVTFQFHIVDMAEPNA
                     FALPGGYIYVSRGLLALTNTEGELAGVVGHEIGHVAGRHAVQRISKQAPMAVVFGLAS
                     GITGLVSQAVGDLIGGVGNLAQSAIFSPYSRSQETEADEVGQKIAAAAGWDPLELATF
                     LGNLEREVTLHEKEPRKPSFFDSHPATPDRVADTTRHAKSLTRATRDPISPTREAYLA
                     RLNGVVVGPRAANGIFRGSAFLHPDLNFFVQFPEKWPHENAPGKIVVAAPKGDAAVVL
                     EGVATGDDPLEGARVLGKAIQVDLA"
     gene            complement(1161..2276)
                     /locus_tag="VLD60_04505"
     CDS             complement(1161..2276)
                     /locus_tag="VLD60_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44249.1"
                     /translation="MRQRKKKAPGRFANALLAGMMLWIVTGCGSIGPTTVNRDRFDYI
                     TAISESWKQQMLLNIVKLRYADVPVFMDVGQVISGYELEGTLTAGGGLLLGDKGAQGN
                     LGDFLNFGAGGRYLDRPTVTYAPLTGADFVKTMMTPFPPGAIMFLVEAGWPVEILLQI
                     GVQSINGLRNQRGGPYAHPPDPEFVEVVRLLSRIQAAGGVGFRVRREKESEEATMMMF
                     HTRRLTPETVKDVAEVRRLLRLDPQATDIQITYGADALSDKEIALHTRSGYQVLIELA
                     SFVTVPPEHVTEHRTLGSAATMQEGVPALPPFVSIQNQTERPADAFARVKYRDHWYWV
                     DDRDFRSKRVFTFLTVLFTLSETGQKIQQPILTIRAN"
     gene            complement(2328..2969)
                     /locus_tag="VLD60_04510"
     CDS             complement(2328..2969)
                     /locus_tag="VLD60_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HSB44250.1"
                     /translation="MYIRHRYAVLFYSLLLTMVGVPIVRALGLTGNLLQVFVGLNLLA
                     AAAPLESRKGHWVLPVILLAAVLSRVGSVQIGHSGLSTASLVLWILIALYAVFRALRL
                     VLRSSTITFEHLYAALSAYLLAGVFLGVLYWSMEEAWPGSVAVSGPAASRPFHLAEGI
                     YFSFITLATVGYGDMVPGNDIVRGLAVVEAIAGQFYLAVMIARLMSLYMREVH"
     gene            complement(3047..3313)
                     /locus_tag="VLD60_04515"
     CDS             complement(3047..3313)
                     /locus_tag="VLD60_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44251.1"
                     /translation="MMRANWTLWILCGMIMLVAGCGGNYYRVNDPAGTREYYTTEIDK
                     TKAGAITFKDEKSGGVVTLQSSEIKEISEEEFKAAVKPEEKKSK"
     gene            complement(3508..4581)
                     /gene="ydiK"
                     /locus_tag="VLD60_04520"
     CDS             complement(3508..4581)
                     /gene="ydiK"
                     /locus_tag="VLD60_04520"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HSB44252.1"
                     /translation="MAERQFELTRDTLAVVFLFALIAASVWILLPFLGAMVWATMIVV
                     STWPVLLTLQRWFLNRRWLAVTTMTLLLLAVFVVPFSILIGALVQNFHQIIDWTTGLS
                     NFTLPPPPDWLEKIPMVGPRMASRWQQFATLSRDEMTTRLSPYLVGVITWVAQQMGGL
                     GSLLVQIILTVFVSSVFYATGESAAAMAVRFGARLAGERGEQAIRLAGHAIRGVALGV
                     VLTALVHALLGGIGLAVVGVPFTVILTAVMFISATVQIGVVPIMLCAVGWLYWTGNSA
                     GGTVLLIWTIAIAPVDNVLRPILIKKGADLPLLLIIAGVIGGLFAFGVVGLFVGPVVL
                     AVSYTLFMAWIDDDRTAKVPAES"
     gene            complement(4746..5456)
                     /locus_tag="VLD60_04525"
     CDS             complement(4746..5456)
                     /locus_tag="VLD60_04525"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSB44253.1"
                     /translation="MNIRRLMVGVVCAAVLTACAHSEQAGGYGKVEPSGFLKDYSKLQ
                     PAKDDTEASLVYFMPDKAKFKAYTKVWLEPVQVWRGEKSDSKNLEQEDALYVSEYLWS
                     RVDEELRKDYMMTREPGPGVMRVRIGITEAGKGIPVLDNLTAAYPGTLVMSKGKKAIG
                     GTESFVGKASVEVEVTDTQTGELIAEAVDRRGGGKYAWKALHRWEDFEQAANYWAKKF
                     RWRACAMRNEAKCEMPEE"
     gene            complement(5622..6344)
                     /locus_tag="VLD60_04530"
     CDS             complement(5622..6344)
                     /locus_tag="VLD60_04530"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSB44254.1"
                     /translation="MRQMMASILALMLVVTGCAATQEAKSVEKSGFLGDYSLLKEGER
                     STVSGGSAENQALLIYKNPAADWRKYKKIQLDPVTVWMSQKDSQLKDVSAEDRQRLAA
                     LLWSKLDEQLRKDYEMTSQPGPDVLRIQVAITEAGSSNAVLDTVTSVIPQTRLLSGMK
                     SLATGVSAFTGSASAEMKVTDSATGTILLEGVDRRGGTKSLRGVTNSWNDVEEAYRYW
                     AEKTRYRLCQWRGGMNCVEPKA"
     gene            6600..7625
                     /locus_tag="VLD60_04535"
     CDS             6600..7625
                     /locus_tag="VLD60_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSB44255.1"
                     /translation="MHQHILNTFFTVCFLITVLTTIPVSVQAAVVVLKNGDRITGLIV
                     KMQDKKLEIDPEYSTTNITIDWGDVRSITSERPMSIQHFGDVELPENVGQRVRDRIIL
                     ETLEEGGPIRLEDVRSINLAEQDYYGYISAGGNQTSGNTETQALNLSGTLTYRKNEHR
                     FILDGKYNRAQADNEDTANNGAINIKYDYFLTRRLFTTASNLSETDQFQNLSLRNTTS
                     VGLGYDILASKQQLLSLAAGPGAVYQDYTTPPATITPSATWGLRAEFRFRGDDVILFH
                     RHQGFKDLGHGSGTRLNADQGIRVTITDNWRVNFEYDIRYNTLPVAGKKTTDTNIIFG
                     FSYDIKP"
     gene            8130..8375
                     /locus_tag="VLD60_04540"
     CDS             8130..8375
                     /locus_tag="VLD60_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44256.1"
                     /translation="MPPAYDIDQKVLEIIVRSQGSMLDDIALQCSDLTWNQIFMAIDR
                     LSREGMLTLMPTGRGLYKVHLSNHARHSASPHQYLSH"
     gene            8421..8675
                     /locus_tag="VLD60_04545"
     CDS             8421..8675
                     /locus_tag="VLD60_04545"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB44257.1"
                     /translation="MSMKSSPAKQTGAKKSSGMMTDQAQAASAISKPAHQRKASFDDL
                     HARISARAYELYAERGYRDGCAQEDWLDAEREILKREFPA"
     gene            8910..9242
                     /locus_tag="VLD60_04550"
     CDS             8910..9242
                     /locus_tag="VLD60_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSB44258.1"
                     /translation="MNTSDDPIARTKKMVILARECSISITQAIAVVLVRIGGTVVDAK
                     LKGKPEKVHWRIKLLTAEGPVKVYVDGRTGEILEAWSEGSFPSADERVSPKELVPGHT
                     EELESAPR"
     gene            complement(9314..11029)
                     /locus_tag="VLD60_04555"
     CDS             complement(9314..11029)
                     /locus_tag="VLD60_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSB44259.1"
                     /translation="MVDTPTTRGNNRNALIGRFAPGLRQLLEYRREWLGIDLVAGVSV
                     AAVAVPTAIAYAQLIGFEPIVGLYAAILPLVAYALFGTSRQLIVNPDAATCAIFAATV
                     LPLAAGDSDALRSLSMALAVLTGLVCIAAGIFRLGFLADFLAKPILVGYLNGVAISIF
                     VGQVGKVVGFAMHSRGMIPMFIELAGKVEQTHLPTLAVGLTTLAVFMGSKRLLPRAPA
                     PLLATVAAVALVYGLGLEDRGVAVVGELPAGLPGLSWPQFDAENLKLLLGGALGVALV
                     SFSSGMVTARSFAARNRYDIDVDQEFIALGACQIASGLSQGFAVTGADSRTAVNDAMG
                     GKSQMVGLIAAATMAAVLFFLTQPLRYLPVAALGAVLIVAAIGLFDIGALRKMWRVNR
                     AECVLSLFTMLGVIWLDLLQGILLAVGSALLLLITRVSRPPDSLLGRVPGMRGWLDMA
                     HTVEAATPPGLIVYRFGAGIVFFNAGYFKKRVMELIAGHSGIQWFILDGSTINTVDVT
                     GAEMLESLVGELASSGVRFGLANVQHGVRNHLERAGVLERIGAEFIFPTLNTASEAFL
                     SRTRT"
     gene            11317..12543
                     /locus_tag="VLD60_04560"
     CDS             11317..12543
                     /locus_tag="VLD60_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44260.1"
                     /translation="MDADQGIRVKIAGNWRVNFGYDPCYKTLPVVAVLRRTRMSSLDS
                     ATISNQKLRLFGLMPCLAMHDLPSRATVTWVARAVFMSVLLAGLWLLPWPVSAEEGWS
                     LEGRGILFYTDDVGLFSATRRLSRDGDPTQPAIDSKLTDQGSDMVFEPLLSVTRSLTN
                     GLGRLDLNVQGQGFVFTDNPQFNHGTLRLQAIQELSSSTRVQARFYYAPNQFLGDNEE
                     RQSGQRQLSAEKLTSYIWSTRLIHDVTPDLSIRLLGRYGLRRYNESFSERNTDFWTIG
                     PHLDWRVAPKIKLGLSYHYERGLAEGRNQPQFEDDVSYINHYLSADVDIDLAEQLSLL
                     TAFHYEHNIWTSSLAGDERNGAHENIYQGEMILVYRVTDSIQGFAGVQRSSRKESFES
                     SSTKNTNVGIGMTAQF"
     gene            complement(12625..13431)
                     /locus_tag="VLD60_04565"
     CDS             complement(12625..13431)
                     /locus_tag="VLD60_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSB44261.1"
                     /translation="MSSSISTVTRAQTARVRTLLKDKRARQAEGAFVVEGANAVRDLL
                     LTHPAKLLKLLITAEYQRRESDQDRRIRQSVDVPSYACTDRMFTTISELEAPQGILAV
                     VRQPSWSEDELFACPTLFGLYGEQLQDPANVGAIIRTAAALNVSALWLTPDSADVYNP
                     KVVRATSGALLSLPIFIAADVTGFVRKDCAIFAAEAGSREAVPMDTIHSVPPRMVLAV
                     GNESRGLSAQTSQLAACRFTIPLSRQVESLNVAATVAIATHYFGGLPKRA"
     gene            complement(13460..13801)
                     /locus_tag="VLD60_04570"
     CDS             complement(13460..13801)
                     /locus_tag="VLD60_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44262.1"
                     /translation="MLGTDIRGIMAEEEEVQRRRQALKSLLSMRTKQLRESLEQRIKR
                     AKSGGDWMQLSREECAMLHKQEKAHLKSQVEQLQHEDDRTRGKLTLLKRAKARAQRIR
                     AAEAASGRKRR"
     gene            13951..14436
                     /locus_tag="VLD60_04575"
     CDS             13951..14436
                     /locus_tag="VLD60_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB44263.1"
                     /translation="MTNSVLSAMAIGSQIQAWRFFRGFSVDRLASEADLSTETIVEIE
                     AEVVDPSTTILESLAAALRVPAAWLFAHPSAFQLLFHEQDEPESLTPDGPDPITERIL
                     TASRMDRSLYVMLTALIQSGEPKLLRAAEMSLKSLVKQSRQSTIPWQNRPSGHFEPPS
                     D"
     gene            complement(14437..15630)
                     /locus_tag="VLD60_04580"
     CDS             complement(14437..15630)
                     /locus_tag="VLD60_04580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HSB44264.1"
                     /translation="MSTLQEVPAARVTLARANEIADSGHLWIYGGFVHAVSGEPAAGD
                     LVDIVMPNGRLYARGLYNPASKIRVRILTFEDEPINESFWRGRIAQAVRLRKRIVSET
                     NAYRMVYGEADRMPGLIVDRYGDVLVMQTLSAGMDRRKELLADLLCEETGASQIYLRN
                     DAKSRALEGLPVATGFLRGEGPTTVEIYEGAARFLVDIARGQKTGWFCDQRENRLAAV
                     RFASGGEVLEVFCHTGAFGLHAALAGAKRVEGLDVSEEALALARRHAELNHVQDRCSY
                     RTADAFDEMRQLDRAGRRYDLVLLDPPAFARSKQAVPRALAGYKDVNLLGIRLAKPEG
                     ILITSSCSHHVSEQDLWRAIGRAAQDAKRPVRLLEQRGQASDHPIMASMPETRYLKCF
                     ILQVL"
     gene            15772..17433
                     /locus_tag="VLD60_04585"
     CDS             15772..17433
                     /locus_tag="VLD60_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSB44265.1"
                     /translation="MGTGKTATIGSAVIDRLHQLGVRHIFGIPGDYVLTLYQLIERSP
                     IRHIGTTREDCAGFAADAYARINGIGAVCVTYCVGGLNCVNAIACAYAERSPVVLLTG
                     SPGLSERTRTPYMHHMVRDFSTQRDVFEKMTVAAVSLDDPLTAEREMDRAFAALLRYR
                     RPIYLEIPRDMVHTPLPSSSHPSCILDEPSDPAALEEAIGEVRTMLAAAKRPCILAGA
                     EIGRFGLHDDLARLVERLNVPIASTLLGKSIIREDHPLYVGVYGGLIARDEVQQFVNE
                     SDCLLILGSILSDVEDVDAQSPLLTEGRTIHATADRVAIKHHRYDAIRFQDFVRAVGQ
                     ASLPSFPSRLLPPPPPIVSKTIAPGAPITLNGLFLHVDTVLNDKTIVIADVGESLFAA
                     ADLHVHRRFEFLSPAYYTSMGFAVPAALGASCADPSLRPIVLVGDGAFQMTGSELSSC
                     VRYGQTPIVIVLNNRGYSTEREILEGPYNDVHEWQYERICDLIGGGVGTRIATQGEFE
                     QGLATALNDKNRIHVLNVLLNPADRSPSMIRLARRLGKKLSTDKP"
     gene            complement(17522..18463)
                     /gene="otsB"
                     /locus_tag="VLD60_04590"
     CDS             complement(17522..18463)
                     /gene="otsB"
                     /locus_tag="VLD60_04590"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HSB44266.1"
                     /translation="MSAGAECAKCDSISPPTTSMPGRIAVSVTRSWAPTDKDMFSPGT
                     ASLRARLTSIGLSRLVLFLDFDGTLVPIARRPELVRMDRAMIRILDRLGRHVPIVVVS
                     GRPVKDLRRVIGLESLHYIGLHGFSYGAPHNPPSWLASRPPRRIVRTWKDALVAVAEK
                     VQGAWVEDKGISVALHNREVAARDRHSLHRNAMNTLQDRLHGKQIRLLRGKHVMEILP
                     ATHCDKGSAVLRLLKRPWARSRVPVYIGDDITDIPALKLVARSGVAIQVGSIPQAPKT
                     WTKLVDTDAVRDLLRYLANQLDRTSAWPSAVPVQRCS"
     gene            complement(18354..19817)
                     /locus_tag="VLD60_04595"
     CDS             complement(18354..19817)
                     /locus_tag="VLD60_04595"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HSB44267.1"
                     /translation="MIVVDRLIVVSNRQPYEHSRENNRLVCKRTDGGLTSALDPVLRR
                     CGGTWIAWGSGSADRDVVGLDMSVEVPPESRAYRLRRVWLTPDEIKDGYLGYANQVLW
                     PLCHITLDRINYLKAFWSGYQSQNVHFSEAVLAELDRETAPVWIHDFHLALLPALVKA
                     AHPTVPVSVFWHIPWPGPDVWRILPERREFLHGLLAADRIVFQTPSYAEAFLQCAQEF
                     LGAIVHSSRDRVLYNGHDTWVSAYPISVDYRTFSAHARSLLVDEAEMVVRERFALPMG
                     MLVGLGVDRLDYTKGLMKRLWALDTFFSKFPEYRGKFTFLQVAVPTRGELDVYQRYRD
                     LIRETVAEINQRHITQFNVEEKGEKAWTPIEFHEGRISFDDLLAMYRMADFALVSSVY
                     DGMNLVAKEYVAAQVHESGVLLVSQMAGAAEELPGAIVINPYETEGVAEAIKGALEMP
                     CDERRRRMRQMRFYLAAHDVHAWADRCLSDAFVGAHR"
     gene            complement(19838..21046)
                     /locus_tag="VLD60_04600"
     CDS             complement(19838..21046)
                     /locus_tag="VLD60_04600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB44268.1"
                     /translation="MAPILAEYQDVAPTGTVDFLRRLGELAEGKTILHVNSTRYGGGV
                     AEILHRLLPLMDELGVKARWEVLTGTDLFYRTTKSFHNSLQGTKQRLTKEMFDAYLEC
                     NRENGRRLNLDADVALIHDPQPAALIDHRSPGSKWIWRCHIDLSTPQRSVWQFLRPYI
                     IRYDAAVFSLPKFAQRLPIPQFLIYPSIDPLSDKNRELPEEEVGAILDRLEVPRDKPL
                     LVQISRFDRFKDPVGVVEAYRIVKKSHDCRLVLAGGSADDDPESAEVLAEVREAVGVD
                     PDVHLLLLPPTANIEINALQRAAAIVLQKSTREGFGLTVTEAMWKGKPVIGGEAGGIT
                     AQVIHGKTGYTVNSPEGAAFYIKHMFNDVELMAGIGRRAKEYARHRFLITRQLQDYLG
                     VMIHLSRQAA"
     gene            complement(21072..21746)
                     /locus_tag="VLD60_04605"
     CDS             complement(21072..21746)
                     /locus_tag="VLD60_04605"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HSB44269.1"
                     /translation="MEDRSADSPFRFVSCMELRESLGKRAQDLERLLEIIEEVPADSI
                     YYHTHSYYLRHPYTQELYPNDFASWVVRHAQDRVLGERLGVVDPFDFDDIETLRAELI
                     GIIGDHLDHTRGAVSTRSTEPFEFIRSHVIEVDLFIEVATLHEFRDELSRVEIGTVYN
                     HICEARMRKRRPSGDFAAWLSSKAGLGLVELGARVVNVGRLGLNLEGMRTRIVNLCDQ
                     ALSAAA"
     gene            complement(21890..23158)
                     /locus_tag="VLD60_04610"
     CDS             complement(21890..23158)
                     /locus_tag="VLD60_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HSB44270.1"
                     /translation="MEQKRVSDFHQNGVVTVLHRLGRPNLEQLEAELQRHAGTNPIAL
                     VLPMLFSELSGPALKGIVETLTQIRYLNEIVISLDRATPLQFRQAKECFSVLPQRVRI
                     VWNDGARIQQILKVLTSHAMDIGLQGKGRGCWTAFGYVLARHQSKVIALHDCDILSYD
                     RQYLARLCYPIAHPNLGYEFAKGYYSRVTNRLHGRVSRLFMTPVLRSLQHLVGFHPFL
                     TFLDSFRYPLAGEFAMVNDLAWTNRIPGDRGLEVGVLAEVYRNCAPRRVCQVDIADAY
                     EHKHQPLSPDDPESGLLRMCVDITKALFRNLANEGVVLSESILKTLRATYLQAAQEAI
                     SRYENDAAINGLGFDRHEERTAVEVFLTGMKIATERFLEDPLGVPMISNWSRVTAAIP
                     DIFGQLIEAVEADHEWEAIAELARAGSREK"
     gene            complement(23324..23539)
                     /locus_tag="VLD60_04615"
     CDS             complement(23324..23539)
                     /locus_tag="VLD60_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44271.1"
                     /translation="MRCQRCQGYMVRDQFMDILNVSGELDFKGWRCLNCGDIVDPVIV
                     RHHLSVGSTTAKARRRWSSVGFVETGS"
     gene            complement(23662..23874)
                     /locus_tag="VLD60_04620"
     CDS             complement(23662..23874)
                     /locus_tag="VLD60_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSB44272.1"
                     /translation="MSTTTFFVGLVAGIGTGILLAPQSGARTRRQLGNLAEDLEEQTS
                     HFLGDAKDSLKKVIRQGKHLVNHAAS"
     gene            complement(24567..26732)
                     /locus_tag="VLD60_04625"
     CDS             complement(24567..26732)
                     /locus_tag="VLD60_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSB44273.1"
                     /translation="MEDVVCRNDQCHIVASSSLTDSHIEVLKQGDTFSLCDQYGDINS
                     LRTGSQGLYHEGTRFLSCFELTINGQRPLLLSSTVKEDNVLLNVDLTNPDMSYDGQTA
                     IGRGALHLSRTRFIGQGLCFERLRVHNYSLLPLPVRLSFSVDADFADLFEVRGKTRPR
                     RGRRLETVTSKDGMSLGYEGLDHVERWLTLRCAPAPTTVDPGMIHCDTLLQAGDAAQW
                     DLTISCEVECRNTHVPTYEHALAEVERALHVARAEDCLVATSNEQFNVWLNRSLADLH
                     MMVSDTPHGPYPYAGVPWFSTPFGRDGLITAFELLWVNPTIARGVLAFLAATQARETV
                     PEQDAEIGKILHETRKGEMAALNEIPFGRYYGSVDATPLFIKLAGTYYERTGDLAFIT
                     QIWPNVELALQWIDEAGDFDHDGFVEYRRRSTGGLVHQGWKDSQDAVFHADGSLAEGP
                     IALCEVQGYVFAAKRRAARLALALGHVEQARRLLKEADNLRAQFERSFWCHDLSSYAL
                     ALDGFKRPCRVRTSNAGHCLWTGIADQRHGMRTAKTLMGEEYFNGWGIRTVAASESCY
                     NPMAYHNGSVWPHDNAVIAAGMASYGFKEGALRILNGLFDASLFLELHRLPELLCGFS
                     RRPGESPTLYPVACSPQTWSSAAVFLLLQSCLGLRIEAPRARLSFSQPVLPPYLECVE
                     IKNLRIGNAVVDLSLERHAHDVSVNVLRRDGRVEIVVTK"
     gene            complement(26742..28043)
                     /locus_tag="VLD60_04630"
     CDS             complement(26742..28043)
                     /locus_tag="VLD60_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44274.1"
                     /translation="MRTWQTLTINRTSTRQNGTTPGAARAVSHWRLLLARDFGLVWFS
                     QLVSQVGDGISNLALLWFVYSITGSPVKTTIIGLLHTIPPIILGPFIGVYVDRLPKKL
                     FLIGSNTLRAVLIGIIPCAISTDTFTVNLLYVLVLLDAVAMAMFSPALTSSVPLIVPR
                     AQFTAANALIQSTTSLGIIFGPVVSGIGIALFGSQEVLCLNAVTYFVSALCLGLVRLE
                     VRDPSAGPKASHGSVLHDLLEGLAFVAIKQRAILLLIVTAGCYGFGAGALTTLFPVFA
                     RKLLGLGPVEVGYLWSSLGVGLLLMSMMLLRFTEWNVSERTRIIAGTSVASALAIGAL
                     VWTRELFLVSLLMIAIGGGMGAFTPIAWGVVQELTPAVMVGRVLGLYNTGAMVATISG
                     ISAFGWVTERFGESTGVAGIGTTFLVTASLGGWLSRSVRSR"
     gene            complement(28040..28801)
                     /locus_tag="VLD60_04635"
     CDS             complement(28040..28801)
                     /locus_tag="VLD60_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB44275.1"
                     /translation="MEMKDKRPLFRRTGIEQSALLLVFCVCALVVSQVTNPMLAQIDQ
                     SSILWAQESKPAQPKTQASKSDESKVKKDNGEEVTDKDKEKDKDKEKDKEKEKEGTGK
                     KAVSSLILTVKLALLEDSRLFPYEIEVEAGSDGIVLSGKVSTEAEKSVAAEIARTVPG
                     VKSVANKIEILKELPEVLARRQDDIITHQVKERFAKSATLKAAGFEVKTEGGVVSLVG
                     TVRFQVIVFEAAEAARQVPGVKAVKTAKVRIESEG"
     gene            complement(28842..>29948)
                     /locus_tag="VLD60_04640"
     CDS             complement(28842..>29948)
                     /locus_tag="VLD60_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44276.1"
                     /translation="IGLLQTIPPLVFGPLIGVYLDRVRRKPVMIGVDLIRTVLVLLIP
                     LLYALDILTLERLYVLVFVTSIVSTIFGPALASSVPLIVSRAQLTAANALIQSTTNIG
                     LLIGPAVGGLGIAMIGAQNVLYLDAATFLVSALCLMPIHLHESPRRTAWQADRTGLVQ
                     DLLVGFRFVFIQHRTVLLLMLTATLYSLGASAFVFLLPVFATQLLDATPVELGWLWSS
                     LGVGMLAASVWLAGVKQGDIRNRLRLISAALAVGAVAVCTLGMLEAPVMAAALIVVFG
                     GSTAVFTPVVWAMLQELTPEHLLGRVFTTFSTGAMASAMAGMAGFGWAADVLGPAVSL
                     VGIGVVFLGTATVAAAFSQRHLHPGTTAPDLLPA"
BASE COUNT         6478 a         9249 c         8082 g         6139 t
ORIGIN      
        1 cggccagatc aacttgtatg gccttgccga gcacccgtgc cccttcaagc gggtcgtcac
       61 ctgtcgcgac gccctcgagc acgaccgctg catccccctt cggtgcggcc accacaattt
      121 tccccggtgc attctcatga ggccacttct ccggaaactg cacgaagaaa ttcaagtccg
      181 gatgcaggaa cgccgatccg cgaaagatcc cgttggctgc gcgcggcccg acgaccacac
      241 cattcaaccg ggccagatag gcttcgcgtg tcgggctgat cggatcccgc gtggcacggg
      301 tcaacgattt tgcatggcgc gtggtatccg caacccggtc aggagtcgcc ggatgcgagt
      361 cgaagaaact cggtttccgc ggttcttttt catgcagcgt cacctcacgt tccaggttgc
      421 cgagaaatgt ggcgagttca agagggtccc agccggctgc tgcggcaatt ttctgcccga
      481 cctcgtctgc ctctgtttcc tgcgagcggc tgtagggaga aaagatggcg ctctgggcga
      541 gattcccgac gccgccgatc agatcgccga ccgcctggct caccaacccg gtgataccgg
      601 aagccagtcc aaacacgact gccatggggg cctgcttcga gatccgctgc acggcatgtc
      661 ttcctgcgac atgaccgatc tcgtgcccga ccacaccggc caattcccct tcggtattcg
      721 tgagggccag cagaccgcgt gacacataga tatatcctcc cggcagcgcg aaggcgttcg
      781 gctccgccat atccacgata tgaaattgga atgtcacgtt cttgcgcggg gactgctcgg
      841 ccagtcgctg gcccagttga tcgaggtacg gggtgaagcc cgattcgtcg gcgaacccca
      901 tccctgcttc gaccttcttg gcttcttctg cgccgagttc ctgttcctgc ttggcggaga
      961 tcagtgtgag ttcgggccgc ccgctaacgg gattgaccgc gcagcctgat cccgcgccca
     1021 caatcatgct cgccaagacg agccagcgca ccgcatcgac gtgtcgtatc catgccatgt
     1081 tcatagtcct gtccccttac cggcagatgt cgtgcggacg ctcctcccgc tcgatttcct
     1141 gctcctgtgc ggcccttcgg ttagtttgcc cgaatcgtca aaatcggctg ctgaatcttt
     1201 tgaccggttt cgctcaacgt gaacagcacc gttaaaaacg tgaagacccg cttggagcga
     1261 aaatcccggt cgtcgaccca ataccaatgg tcccgatatt taactcgcgc aaatgcgtct
     1321 gcgggccgct cggtttggtt ttgaatcgac acgaacggcg gcagcgccgg tactccttct
     1381 tgcatcgtgg ccgccgaacc cagcgtgcga tgctccgtga cgtgttccgg aggcacggtc
     1441 acgaacgagg cgagttcgat caggacttga tagccggatc tggtatgaag cgcgatctct
     1501 ttgtcgctca gcgcatcagc tccataggtg atttggatgt ccgtcgcctg ggggtccaac
     1561 cgcaacagcc gcctgacctc cgcgacatcc ttcacagtct ccggagtcaa gcggcgcgta
     1621 tgaaacatca tcatggtggc ttcctcgctc tccttctcgc gtcggaccct aaagccaacc
     1681 cccccagccg cttgaatacg actcagtagc cggacgacct cgacgaactc cggatcaggc
     1741 ggatgggcat atggcccgcc tctctggttt cggaggccat tgattgactg cacgcctatt
     1801 tgcagcagaa tttccacggg ccacccggcc tcaaccagaa acatgattgc tcccggtgga
     1861 aacggcgtca tcatggtctt gacgaagtcc gcgcccgtaa gcggcgcata ggtcacggtc
     1921 ggccgatcga gatagcgccc cccggcgccg aaattcagaa agtctccgag gtttccctgc
     1981 gcgcccttgt cgccgaggag gagacccccg ccggctgtga gggtgccttc gagttcatag
     2041 ccgctgatca cttggcccac atccataaac acgggcacat ccgcataccg cagcttgacg
     2101 atgttcaaca gcatctgctg cttccaggat tcactgatcg cggtgatgta atcgaaccgg
     2161 tctcgattaa cggtggtcgg accaatgctc ccgcagccgg tgacgatcca gagcatcatt
     2221 ccggccagca gcgcatttgc aaaccggcca ggcgctttct tttttctctg tcgcacgaat
     2281 tcttctcctt ccatccaacc cctctcacct gcgaaatcca caacgtttca atgcacctcg
     2341 cgcatgtaga ggctcatcag acgcgcgatc atcacggcga ggtagaactg ccccgcaatc
     2401 gcctccacca ccgccagccc tcgcacgatg tcatttcccg gaaccatatc gccgtagccc
     2461 acggtcgcga gcgtgatgaa gctgaagtag ataccttctg ccagatggaa cgggcgagac
     2521 gccgccgggc cgctgacggc caccgagcct ggccaggcct cttccatcga ccagtacagg
     2581 acgcccaaga aaacccccgc cagcagatag gcgctcaatg cggcatagag atgttcgaac
     2641 gtaatggtag acgagcgtag aacaaggcgc agggcacgga ataccgcata cagcgcgatc
     2701 aggatccaga gtacaaggct tgcggtcgag agtcccgaat ggccgatctg aacggaccca
     2761 actctgctga ggacagcggc cagcagaatc accggcagaa cccagtgccc ttttcgactt
     2821 tcgagcgggg cggcggcggc cagtagattc aagcccacaa acacctgcaa gagattgccg
     2881 gtcaatccga gggctctgac gatcggcact ccgaccatgg tcaacaagag cgaatagaac
     2941 aggacggcgt agcgatgccg aatatacatt cgccaccact ccgagagtcc acccggatgc
     3001 accgaaccgc ttgaggcgtg ctgcgtcatt tcctcagcga cccgggttat ttgctctttt
     3061 tctcctcggg cttcaccgcc gccttgaact cttcctcgga aatctccttg atctccgacg
     3121 attgcagcgt caccacaccg ccactcttct catctttgaa ggtaatggcc ccggccttgg
     3181 ttttgtcgat ttccgtggtg tagtactccc tggtaccggc cggatcgttg acgcgatagt
     3241 aattcccgcc gcagccggcg acgagcatga tcattccgca gagtatccaa agggtccaat
     3301 tcgctctcat catctggtcc tccaacgtta aaaaaagcgc acagtgctct gttgtacgag
     3361 ggcattgtca cgatgtcgag ccgccgggac cggagatcac tccttgcgat ccccagttca
     3421 gggccaaggc gaagatcggc cgaccttata ccacacctgt cgaaacaatg gaccagtcgg
     3481 cgcgacgacg atttcttccg agagccttta gctttccgca gggaccttgg cggtccgatc
     3541 atcatcgatc caagccatga acaacgtgta ggacactgcg agcacgaccg ggccgacgaa
     3601 cagaccgacc acgccgaatg cgaacaagcc cccgatcacg ccggcaataa tcagcaggag
     3661 tggaagatcc gctcccttct taatcagaat gggccgaagc acgttatcga cgggggcgat
     3721 cgcgatggtc catatgagca gcacggtccc cccggcggag ttgccggtcc agtacagcca
     3781 gcccaccgca cacaacatga ttgggaccac cccaatctgc acggttgccg aaatgaacat
     3841 caccgcggtc aagatgacgg tgaacggcac gcccacgacc gcgaggccga tcccgccgag
     3901 caacgcatgt accaatgcgg tcaacaccac tccgagcgcg accccccgga tcgcgtgtcc
     3961 agcaagacga atggcctgct ccccccgctc tcccgcgagt cgagcgccga atcgcaccgc
     4021 catcgccgcg gcggattccc ctgtcgcgta gaacacggac gagacgaaca ccgtcaagat
     4081 gatttggaca agcaacgatc caagaccgcc catctgctgg gccacccatg taatgacacc
     4141 gaccaggtac ggtgacaggc gagtggtcat ttcgtcacga ctcaacgttg caaattgctg
     4201 ccagcgcgac gccatgcggg gaccaaccat gggaatcttt tccagccagt cgggtggtgg
     4261 gggcagagtg aagttggaga gtcctgttgt ccagtcaata atctgatgga agttctgcac
     4321 gagggcgccg ataagaatcg agaagggtac gacgaacacc gccaacaaga ggagggtcat
     4381 tgtcgtcacc gcaagccacc gccggttcag gaaccagcgt tgaagtgtga gcaatacggg
     4441 ccatgtggag acgacaatca tggtggccca gaccatggct ccaaggaacg gtaacaggat
     4501 ccagaccgac gcggcgatca aggcaaagag gaacaccact gccagcgtat cgcgggtcag
     4561 ctcaaactgc cgctctgcca tggatacacc tcataaattc cggtgggtta gcccaaaatt
     4621 gatgggccgc gattgactgt tcatatgcac agtagtaggg ccgcacccgc acgtagacgg
     4681 atgcggcccc gctctctcac tcggcatcct gccgacgcca cggcgcggac gctaatccaa
     4741 cagggctatt cctccggcat ctcgcacttg gcctcgttgc gcatggcgca ggcccgccag
     4801 cgaaacttct tggcccaata gttcgcggcc tgctcgaaat cttcccagcg atgcagcgct
     4861 ttccaggcgt acttccctcc gccgcgccga tccaccgctt ccgcgatcag ttcaccagtc
     4921 tgagtatctg tcacttctac ttccaccgac gccttcccga caaaactctc ggtcccgccg
     4981 atggctttct tccccttgga catcaccagc gttcccggat aggcagcggt caggttgtcc
     5041 aggactggta tgcccttgcc cgcttccgtg atcccaatgc gcacccgcat gaccccaggc
     5101 cccggttctc gcgtcatcat atagtctttg cgcaattctt cgtcgacacg cgaccagaga
     5161 tattccgaca catagagggc atcctcctgt tcaagatttt tgctgtcgga cttctcccca
     5221 cgccagacct gcaccggctc cagccacacc tttgtgtagg ctttgaactt ggccttgtcc
     5281 ggcatgaaat agaccagcga cgcttccgta tcatccttcg cgggctggag cttggaatag
     5341 tctttcaaga acccggacgg ttcgaccttc ccatatccgc ccgcctgttc tgagtgggca
     5401 caggcggtga gcactgccgc gcagacaacg ccgaccatca atcggcggat gttcatgaac
     5461 aggtcccttt cttctgagcc ggcacctctc atgatcgacc attcacccct gtgagcttga
     5521 tcgatgatga aggctcagcc cattgaccga tcgtatcgac gattgctcac gatccgctga
     5581 gatcccgcag gacctcagcg gacaattgcc tcagtcgccc atcaggcttt cggctcgacg
     5641 caattcatcc cgccacgcca ctgacacaag cgataccgcg tcttctcggc ccagtagcga
     5701 taagcctcct ccacgtcgtt ccaagagttg gtcacacccc tcaggctctt ggtgccaccc
     5761 cggcggtcca ccccctcaag caagatcgtt ccagtggcac tgtcggtgac cttcatctcc
     5821 gcgctcgcac tgcccgtgaa cgccgacacc ccggtcgcga gactcttcat gccggaaagc
     5881 aaccgtgtct gtgggatgac gctcgtcacc gtatccagga cggcattgga tgatcctgct
     5941 tcggtaatgg caacctgaat ccgcaggaca tccggaccgg gttggcttgt catctcgtaa
     6001 tcttttctga gttgttcgtc gagtttcgac cacaacagcg ccgccagcct ctgtcgatcc
     6061 tcggctgaca cgtccttgag ctgcgagtcc ttctggctca tccagaccgt cacgggatcc
     6121 aactgaatct tcttgtactt gcgccaatcg gccgcaggat tcttgtagat caacaacgcc
     6181 tgattctcgg cgctcccgcc gctcaccgtg ctgcgctccc cctccttcag cagagaataa
     6241 tccccgagga acccggactt ttccaccgac ttcgcctctt gcgtcgcggc gcagccagtc
     6301 accactagca taagtgccag aattgatgcc atcatttgac gcatgctcgc ctcctcacta
     6361 gttatcccga taccgcggca atcgcggtta atttgggcgt atggtagcca ccttaaacaa
     6421 acaacgcaag tcgtttttcc gacgacttgc gaatgccgtt atcttgaaag atcagcaggt
     6481 gttccgtaga atgcgcactt catacattgg cgcacgttgc acgcagccgc aaatcttccg
     6541 gctaccggac atagcgcggt tctcttgctc accactgcac gactcgcaca cattaatcga
     6601 tgcaccagca tattctaaat accttcttca cggtctgctt ccttatcact gtcctcacaa
     6661 caattcccgt ctcggtccag gccgcggtcg tcgtgctcaa gaacggcgat cgaatcaccg
     6721 gtttgatcgt caaaatgcag gacaaaaagc tcgagatcga tcccgagtac tcgaccacta
     6781 acatcacgat cgactgggga gacgtccgga gcatcacatc ggaacggccg atgtcgattc
     6841 aacattttgg ggacgtggag ttgcctgaga acgtcggaca gcgcgttcgc gaccggatca
     6901 tcctggagac cctcgaagag ggggggccga ttcgcctcga agatgtgcgg agtatcaatc
     6961 tcgcggaaca ggattattac ggctacatct cggcgggcgg caatcaaacc tccggcaaca
     7021 cagaaacgca ggcactgaac ctctcgggca cgctcaccta ccgcaaaaat gaacaccgct
     7081 tcatcctgga tggaaaatat aatcgcgcac aagccgacaa cgaagacacg gccaataatg
     7141 gcgcgataaa tattaaatac gattattttc tcacccgccg actcttcaca accgcctcga
     7201 atctctcgga aaccgaccag ttccagaact tgtcgctacg caatacgaca agtgtcggcc
     7261 ttggctacga catcctcgcg agcaaacaac agctgctatc cttggcggct ggcccgggag
     7321 cggtgtacca ggattacacc acgccccctg ccacgattac tccctccgcc acgtgggggc
     7381 tacgggctga attcagattc cgcggcgatg acgtgatcct cttccatagg catcaaggct
     7441 ttaaggacct ggggcatggg tcaggaaccc gcttgaatgc cgatcagggg atccgggtga
     7501 cgatcacgga taactggcgg gtcaattttg agtatgacat ccgctacaac accctgccgg
     7561 tggcaggcaa gaagacaacg gatacgaata tcatttttgg attcagctac gacatcaaac
     7621 cataagctaa gcctgtcttt tgggcctctc gcctgtgtct tacgatgcac gatcgccgag
     7681 caggcgctcg gataccctgc cggtgcgtgc ggtcgtgctg ttcctgcttc tcacaggtct
     7741 gtggctcctg ccagagccgg acgctgcaga agaaggaaag gcctcagccc aatcaccaac
     7801 gattcttgga ccggcctatc cgctcgctgt ttcttaaaca ctcgcttggg ttcggaagaa
     7861 cttcagctgc ttactccaat cacgttggaa acgtcctgtt gcctggccaa acggacacgg
     7921 ctgaggaaaa cagctacagc tttcctataa gtagttgccc caaatcaccc cacccctcag
     7981 agtgatgtcg tcgcatcgtt tgcctcgcga ggcacggaag ctcttgttat tccagttttc
     8041 ccgcttgtca tcgtcctcca aacccagcaa caggcattga agctgcactg gacgctgacc
     8101 atgcaccaaa ctaatcggga ggtacggcta tgccgcccgc atatgacatc gatcaaaagg
     8161 tgctggaaat catcgttcgg tcacagggca gcatgctgga tgacatcgcg ctccaatgct
     8221 cggacctcac ctggaaccaa atcttcatgg cgattgatcg tttaagtcgc gaagggatgc
     8281 tcacgctgat gcccacaggg cgaggtctct ataaggttca tctgtcaaac cacgcacggc
     8341 acagcgcatc accgcatcag tatttgagtc actgagtagg tactcgtgcc tgaagtcaat
     8401 ccacagatga aaggagtagg atgagcatga agtcctcgcc ggcaaaacag acaggagcga
     8461 agaagtcgtc cgggatgatg accgaccagg cccaagcagc ctcggccatt agcaaacccg
     8521 cacatcagag gaaagcctca ttcgatgatt tgcacgcccg catatccgca cgcgcgtatg
     8581 agctgtatgc cgaacggggc tacagagacg gctgtgcaca ggaggattgg ctggacgcgg
     8641 aacgggaaat tttaaaacgt gaattcccag cttagacagc ggatgcaacc tcgaccttgc
     8701 tcaggttagc cccggcaggt ttcgatctgc atcaagcgga acggaggtcc cgacgcgatc
     8761 ttcggcacga tccatgcgcc ttgacagatc attcgcgttt gacatagcct taatgaccgt
     8821 ctcagccggt ccttcatctt attcttcagt tattgcagtg agattctggc attcggcagc
     8881 ttcagaacga cctactactt ggaggtgcca tgaacactag cgacgatccg attgcacgaa
     8941 ccaagaaaat ggttattctg gctcgcgaat gttccatctc gatcactcag gcgatcgcag
     9001 tcgttctggt gcgtatcgga ggaacagtag tcgacgcgaa gctgaaagga aagcctgaaa
     9061 aagttcactg gaggatcaaa ttactgaccg cagaaggccc cgtcaaggtc tatgttgatg
     9121 gacgtaccgg cgagatcctt gaggcgtggt ctgaaggctc gttcccctcg gctgacgaga
     9181 gagtgagtcc gaaggaactc gtgcccggtc atacggaaga gctcgaatcg gctccacgat
     9241 aatccccccc tcgccctcat cactccagag ttcctggata tccactcccc tgttccagcc
     9301 ctgcgggacc ttctcacgtc cgcgtgcggg agaggaatgc ttcgctcgcg gtattgagcg
     9361 ttggaaagat gaactcggcg ccgatacgtt ccaatacacc ggcacgctca aggtggttgc
     9421 gcaccccatg ctgaacgttc gcaaggccga agcggacgcc gcttgaggcc agttctccga
     9481 caagggattc gagcatttcg gccccggtga cgtctaccgt attgatcgtg cttccatcca
     9541 gaatgaacca ttggatgccg gagtggccgg cgatgagttc catgacgcgt ttcttgaagt
     9601 accccgcatt gaagaagacg atcccggcgc cgaaccgata gacgatgaga ccgggagggg
     9661 tcgcggcttc cacggtatga gccatgtcga gccatcctct catccccggt acacgtccca
     9721 acaagctatc cggcggtcgc gaaacgcgcg tgatcagcaa gagcagcgcg ctccccaccg
     9781 ccaagagaat gccctgcagc aaatccagcc agatgacccc gagcatcgtg aaaagggaca
     9841 agacgcactc cgctcggttc acacgccaca tcttgcgcag cgcgccaata tcgaaaagtc
     9901 cgatagcagc cacgatcagc accgcgccca acgccgccac cggcaaataa cgcaggggct
     9961 gggtcaggaa gaagagcacc gccgccattg tcgcggccgc gatcagccca accatttgag
    10021 atttgccgcc catggcatcg ttgactgcag ttcgcgagtc ggccccggtg acggcgaacc
    10081 cctgcgacaa gccggaggca atctggcaag caccaagcgc gatgaactcc tggtcgacgt
    10141 cgatgtcata gcggttccgc gcggcaaagc tccgcgccgt caccattccg ctgctgaagc
    10201 tcaccagggc cacgcccaag gcaccgccga gaagaagttt caggttttcc gcatcgaatt
    10261 ggggccagct caggcccggc agtcccgccg gtaactcgcc cacgaccgcc acacctctat
    10321 cctccagccc gaggccatag accagcgcca cggccgccac cgtggccagt aacggcgccg
    10381 gcgcgcgagg caacaagcgt ttgcttccca taaatacagc gagggttgtc aatcccacgg
    10441 caagcgtcgg cagatgggtt tgctccacct tcccagctag ctcgatgaac atcgggatca
    10501 taccgcgcga gtgcatggca aagccgacca ctttgccgac ctggccgaca aagatgctga
    10561 tggccacgcc attgagataa ccgaccagga tcggttttgc gagaaaatcg gcaaggaagc
    10621 cgagtcggaa gatccccgca gcgatgcaca ccagcccggt caataccgcc agcgccatgg
    10681 acagtgaccg gagggcgtcg gagtcgccgg cggcgagcgg aagcactgtg gcggcaaaga
    10741 tggcgcacgt ggctgcgtcg ggatttacga taagttgacg cgaggtaccg aacagcgcat
    10801 aggctacgag cggaagaatg gccgcataca gcccgacgat cggctcgaag ccgatcagtt
    10861 gcgcgtaggc aatcgccgtg ggcactgcca cggcagcgac cgaaacgccg gccaccaggt
    10921 cgatccctag ccattcacgt cggtactcca ggagttgccg aagaccaggc gcaaaacgtc
    10981 cgatcaacgc gttgcggttg ttccctctag ttgttggggt atcaaccatt tcaatggtgt
    11041 ccggattcag taattctcag agaaaatgca ttggctcttg gggaatgccc cattatcgtg
    11101 ctcgccgctg atcgatcggc tggaccgttt catcttgtcg cgtggcattt ttgtcggcca
    11161 tccactccag cacgagagaa ctgtggctga ggctcgtgct tcgtggcgtg cctcgcaagt
    11221 gagtagcatc caactcgggg caatcatacc tgtccttttg agattagtga aggttgacca
    11281 gcccgcccac acgggccact tgcttcagcg actcgcatgg atgccgatca agggattcgg
    11341 gtaaagatcg cgggaaattg gcgggtgaat tttggatatg atccctgtta caagactctt
    11401 cctgtcgtcg ccgtattaag acggacacga atgtcgtctt tggattcagc tacgatctca
    11461 aaccagaagc tgcgtctctt cggactcatg ccctgtcttg ccatgcatga tcttccatca
    11521 cgcgctacgg taacctgggt tgcgcgagcg gtcttcatgt ccgtgcttct cgcaggtctg
    11581 tggctcctac cctggccggt ctctgcagaa gagggatggt cgctcgaagg tcgggggatt
    11641 ctcttttata ccgatgacgt cggattgttt tccgccaccc gccgcctcag tcgggacggc
    11701 gatccgaccc agccggccat agattcaaag ctgacagacc agggatcgga tatggtcttc
    11761 gagcccttgt tgagcgtgac gcggtcgttg acgaacggcc ttggacggct cgacctgaac
    11821 gtgcaaggcc agggcttcgt cttcacggac aatccccagt tcaaccacgg cacgctgcgg
    11881 ctccaagcga ttcaagagct atcctccagc acccgcgtgc aggctcggtt ctactatgcc
    11941 ccgaaccagt ttctcggcga caacgaagag cgccagtcag gccagcgtca gctgtcagcc
    12001 gaaaaattga ccagttacat ctggtctacc cgtctcattc atgatgtgac gccggatctt
    12061 tcgatccgat tgctcggacg atatggcctg cggcgttaca atgaatcttt ttcggaacgc
    12121 aacaccgact tctggacgat cggccctcat ctggactggc gggtcgcgcc gaaaattaag
    12181 ctcggcctga gctatcatta tgaacgcgga cttgcggagg ggcgcaacca accgcagttc
    12241 gaggacgacg tatcctatat caaccattac ctgtccgcgg acgtcgacat cgatctggcc
    12301 gaacagctct cgctcctcac agcgtttcat tatgaacaca acatctggac cagcagtctg
    12361 gcaggcgatg aacggaacgg tgcccatgaa aatatctacc agggcgaaat gattctggtc
    12421 tatcgcgtga cggattcgat tcagggattc gccggggtcc agcgttcaag ccggaaagag
    12481 agtttcgagt cgagttccac caagaacacc aacgtcggca tcggcatgac cgcgcaattc
    12541 tgatgcggcc gcggatcgtg accgggccgg tagtttctgg tcatggacgc aagacgtttg
    12601 agcagtgagg ctgagggagg aaggttacgc ccgtttcggg aggcccccga agtaatgcgt
    12661 cgcaatggcc acggtggccg caacgttcaa agactcaacc tgtcggctca acggaatcgt
    12721 gaaccggcag gcagccagct ggctcgtttg ggcggatagt ccgcgacttt catttcccac
    12781 agcgagaacc atgcgcggcg gaacggaatg aatagtgtcc atcggcactg cttcacgact
    12841 tccagcttcc gcagcgaaaa tcgcgcagtc cttcctgaca aatcccgtga catcggccgc
    12901 gatgaaaatc ggaagggaca acagcgcccc gctcgtggct cgtacgactt tgggattgta
    12961 cacatcggcc gaatccggcg taagccagag cgcgctaaca ttcaacgccg ccgctgtgcg
    13021 aatgatcgcc ccgacgttcg ccggatcctg caactgctcc ccgtataacc cgaacagtgt
    13081 tgggcaagcg aacagctcgt cttcgctcca gctcggctgc cgcacgaccg cgagaattcc
    13141 ttgaggagct tcgagttccg agatggtcgt gaacatccga tccgtacagg catatgacgg
    13201 gacatcaacc gattgccgaa tccgccgatc ctggtcgctt tcgcgccgtt gatactcggc
    13261 agtgatcagg agcttcagga gtttggcggg atgggtaagg agcaggtcgc gaacagcgtt
    13321 ggcgccttcg acgacgaagg cgccttctgc ttgtcgagcc cgcttatctt tgaggagagt
    13381 tcgaacgcgc gcggtttgcg cgcgcgttac ggtagagatg gaagaagaca cgccaggatg
    13441 cggtaccaat cagcgccggt tatcgacgct tacggccgga tgccgcctct gccgctcgaa
    13501 tccgttgggc gcgagccttc gcccgcttga gcagcgtcag tttgccgcgc gtacggtcat
    13561 cttcatgttg caactgttcg acctgggact tcaggtgagc cttctcctgc ttgtggagca
    13621 tggcacattc ctcacgcgaa agctgcatcc aatcgccccc gctcttggcc cgcttgatcc
    13681 gttgttccaa cgactcacgc aactgctttg ttcgcatgga aaggagcgac ttcagtgcct
    13741 gcctgcggcg ctgaacttcc tcctcctcgg ccatgattcc acggatatcg gttcccaaca
    13801 tgatgcgcct cctcacaaca tcaaaaatag actaatgtgc ttattctacc tgatttgatt
    13861 actcatcttc cactacgaga attcttccat aacatattga tcataaaggc ataataccca
    13921 ttgaagaccg gaaatcccgg ttgcagagga atgacaaatt cagtactatc ggccatggca
    13981 atcggctcac aaatccaggc ctggcgcttc tttcggggtt tttccgttga tcgtttagcc
    14041 agcgaagccg atctttccac tgagaccatc gtggaaatcg aggcagaagt cgttgatccc
    14101 tccaccacta tattggaatc tctcgcagcg gctctgagag ttcctgctgc ctggctcttt
    14161 gcccatccca gtgcttttca actgctcttc cacgaacagg acgaaccaga aagtcttact
    14221 ccggatggtc cggatcccat caccgaacga atcctgaccg cgtctcgaat ggaccgatca
    14281 ctctatgtaa tgctgaccgc actgatccaa agcggtgaac ccaagctgct ccgggcggca
    14341 gaaatgagtc tcaagagcct ggtcaagcaa tcgagacaat caaccattcc ctggcagaat
    14401 cgcccttcag gccacttcga accaccgagt gattgattac aagacttgta ggatgaagca
    14461 cttgaggtag cgggtctccg gcatggaggc catgatggga tgatcactcg cctgcccccg
    14521 ttgttcgagc aaccggactg gccgtttcgc atcctgcgca gcgcgaccga tggccctcca
    14581 gagatcctgc tctgatacat gatgggaaca ggaactcgtg atcaatattc cttccggctt
    14641 cgccagccgg ataccgagca gattcacgtc cttataccct gccaacgcgc gcggtaccgc
    14701 ctgcttgctc cgggcaaagg cgggaggatc gaggaggacg agatcataac gtcgaccggc
    14761 ccggtctaat tgacgcatct cgtcgaacgc atcagcggtt cgataggagc agcggtcctg
    14821 cacatgattg agttcggcgt ggcggcgagc cagggcaagc gcctcctcac tcacatccaa
    14881 cccttctacc cgcttcgcac cggccagtgc cgcatgaagt ccgaaagctc cggtgtgaca
    14941 gaacacctcc aacacttcgc cgccggaagc aaaccggacc gcggcaagcc ggttctccct
    15001 ctggtcgcag aaccagccgg ttttctggcc gcgagcgatg tccacgagaa atcgtgcagc
    15061 accctcgtag atctctaccg tcgtgggacc ttcgccgcgc aagaatcccg tcgccacggg
    15121 aagtccttcc agcgcccggc tcttggcgtc gttgcgaaga tagatctggc tcgcaccggt
    15181 ttcctcgcag agaagatcgg cgaggagttc tttccgtcga tccatcccgg ccgacaatgt
    15241 ttgcatcacc aagacgtcgc cgtaccggtc cacaatcagg ccgggcatgc gatcggcctc
    15301 tccatagacc atccgatagg cattcgtctc ggatacgatt cgcttccgga gccgcacggc
    15361 ctgcgcaatt ctgcctctcc agaatgattc gttgatcggc tcatcttcga aggtgaggat
    15421 gcgcactctg atcttcgagg cgggattgta cagaccgcgc gcgtacaatc gaccgttggg
    15481 catgacgata tcgacgagat cgcctgctgc aggttctccg cttacggcat gaacaaaacc
    15541 gccatagatc cagagatgcc ccgagtcggc aatctcgttg gcccgcgcca gtgtcacccg
    15601 cgccgctgga acctcctgta acgtagacat ggcttactta tccgatgctg gaggccgaag
    15661 gtcaagccct cctcatggcc gattgttgct tgacggcccg cgcgctctgt ggtttgctga
    15721 atctcggcgc tgcgtgattc gcgctatcgc gacgagcaag gagagtcgag catgggaacg
    15781 gggaagacgg caacgatcgg ctcagcagtc atcgatcggc tgcatcagct gggtgtccgt
    15841 cacatcttcg gaatccctgg cgactacgtc ctgactctct atcaactcat cgaacgctcc
    15901 cccattcgac acatcgggac gacccgggaa gactgcgccg gcttcgccgc agacgcctat
    15961 gcacgcatca atggaatcgg cgccgtgtgc gtgacctatt gcgtcggcgg attgaactgc
    16021 gtcaatgcca tcgcctgcgc ctacgctgaa cgctcgcccg tcgtgctgct caccggttct
    16081 ccgggactct ccgaacggac gcggacgcca tacatgcatc acatggtgcg ggatttttcg
    16141 acgcagcgcg acgtcttcga aaaaatgacc gtcgccgccg tgtccctcga tgatccactg
    16201 accgccgagc gtgaaatgga tcgcgccttt gccgccctcc tccgctatcg acgaccgatc
    16261 tatctcgaaa tccctcgcga catggtccat accccgcttc cgtcctcatc ccatccctcc
    16321 tgtattctgg atgagccgag cgatcctgcc gccctcgaag aagcgatcgg cgaggtccgc
    16381 accatgctgg ccgcagccaa gcggccctgc atcctcgccg gggctgaaat cggacggttc
    16441 gggctgcacg acgatctggc gcgcctcgtc gaacggctga acgtgccgat cgcctcgacg
    16501 ctgttgggaa agtccatcat ccgagaggac catccactct atgtcggtgt ctacggcgga
    16561 ctcattgctc gcgacgaagt gcagcaattc gtcaacgagt ccgactgcct gctgattctt
    16621 ggatcgattc tgtccgacgt cgaagacgtg gacgcacaat cgccgctgct gacggaaggg
    16681 cgaacgattc acgcgaccgc cgatcgggtt gccatcaagc accaccgata cgacgcaatc
    16741 aggtttcaag acttcgttcg ggccgtcggc caggcctcgc tcccctcgtt cccctcgcgg
    16801 ttgctaccgc cgcctccgcc tatcgtatcg aaaacgattg cccccggcgc gcccatcacg
    16861 ttaaacggcc tgtttctgca cgtggacacc gtcttgaacg acaagacgat cgtgatcgcg
    16921 gacgtcgggg aatcgttgtt tgccgccgca gacctccatg tgcatcgcag attcgaattt
    16981 ttgtcgccag cctactatac gtcgatgggc tttgcggtcc ccgccgctct gggcgcgtcc
    17041 tgtgccgacc catcactccg gccgatcgtt ctcgtcggcg acggtgcatt tcaaatgacc
    17101 ggttccgagc tgtccagttg cgtccgatac ggccagactc ccattgtcat cgtcttgaat
    17161 aatcgcggtt attcaaccga acgggagatt ctcgaaggcc cctacaacga cgtccacgaa
    17221 tggcaatatg aacgaatctg cgacctgatc ggtggtggag ttggaacacg gatcgcgaca
    17281 caaggagagt ttgagcaagg acttgccact gccctgaacg acaaaaaccg gatccacgtc
    17341 ctcaacgtac tgctgaatcc cgccgaccgt tcccccagca tgatccgcct ggccaggcgc
    17401 ctcggcaaga aactgtcgac agataaaccg taattcatgc agagcgggat caggacctct
    17461 cacaaggtgc cccccgacga gagtcgtgca gagagtcagg gggctcttcc ctcctctatg
    17521 atcacgaaca tcgttgaacc ggtacggctg aaggccaggc acttgtgcga tcgagctgat
    17581 tcgccagata gcggagaaga tcccgaacgg cgtcggtatc gaccagtttc gtccaggttt
    17641 tgggggcctg cgggatcgat ccgacctgga tcgcgacccc cgatctggca acaagcttga
    17701 gcgccggaat gtccgtgatg tcatcaccga tgtataccgg gactcgagat cgcgcccagg
    17761 gtctcttgag caaccgcaac acggcacttc ccttgtcaca atgggtcgcc ggaagaattt
    17821 ccatgacgtg tttccctcgc aacagacgga tctgcttccc atgaagccgg tcttgcaagg
    17881 tattcatcgc gttgcgatgt aaactgtggc gatcccgcgc cgcgacttct cgattgtgca
    17941 atgccacgga gatccctttg tcttctaccc acgcaccctg gactttttcc gctacggcaa
    18001 ctagtgcgtc cttccatgta cgaacgattc gacgcggcgg ccgagaagcc aaccatgaag
    18061 gggggttgtg aggcgccccg tatgagaagc cgtgcaggcc tatataatgg agggactcga
    18121 gacctattac gcggcgcaaa tccttcaccg gacgaccgct cacgaccacg atcggaacat
    18181 gtcgccctaa ccgatcgagt atccgaatca tggccctgtc cattctgaca agttccggcc
    18241 ttctggcaat cggaaccaac gtcccgtcga aatcgagaaa cagcaccaac cgggacagac
    18301 ctatcgacgt aagccgtgca cgtagtgatg ctgtgcctgg cgagaacata tctttatcgg
    18361 tgggcgccca cgaacgcgtc actgagacag cgatccgccc aggcatggac gtcgtgggcg
    18421 gcgagataga atcgcatttg gcgcattctg cgccggcgct catcacacgg catctccaat
    18481 gcccctttga tggcctccgc gacaccttct gtctcgtaag ggttgatcac gatggcaccc
    18541 ggcagttctt ctgccgctcc ggccatctga ctcacgagca gcacgccgct ttcatgcact
    18601 tgcgcggcaa catattcctt ggccacgagg ttcatcccgt cgtacacaga actgaccaac
    18661 gcgaaatctg ccatgcgata catggccagg aggtcatcga aactgattcg cccctcatga
    18721 aattcgatgg gagtccaggc tttctccccc ttctcctcga cattgaactg agttatgtga
    18781 cgctgattga tttcggccac cgtttcccta atcaagtcgc ggtagcgctg gtagacgtcg
    18841 agctcgccgc gtgtggggac ggcgacttgg aggaaggtga atttcccacg atactcggga
    18901 aactttgaga agaatgtatc aagtgcccac agtcgtttca tcagtccctt cgtataatcc
    18961 aaccggtcaa ctcccaatcc caccagcatt cccattggga gggcgaaccg ctctcgcacg
    19021 accatttcgg cttcatccac gagaaggctg cgcgcatgcg cggagaacgt tcgatagtct
    19081 acgctaatcg gataggctga cacccaggta tcgtgcccgt tgtacagcac gcggtctcga
    19141 gaggaatgaa caatggcccc aaggaattcc tgcgcgcatt gaagaaacgc ttcggcataa
    19201 ctgggagtct ggaacacgat ccggtcggcc gccaggagcc catgcaggaa ttcccgacgt
    19261 tcggggagaa tacgccatac atccggcccg ggccagggga tgtgccagaa gacggacacc
    19321 ggtacggtcg ggtgtgccgc tttcaccagc gcgggtagaa gcgccagatg gaaatcatgg
    19381 atccagacgg gcgccgtctc tcggtccaat tcagccaaca cggcttcgga gaaatggaca
    19441 ttctgcgact ggtagccgga ccaaaatgct ttcagatagt tgatacgatc caacgtgatg
    19501 tgacagagcg gccacagtac ctggttggcg tatccgaggt agccatcctt gatttcatcc
    19561 ggagtcagcc agacgcgccg aagccgatag gctcgcgatt cgggaggaac ctcaacggac
    19621 atgtccagcc ctaccacatc ccgatcggcg gatccgcttc cccaagcgat ccacgttcca
    19681 ccgcaccgtc gaagtaccgg gtccagagcc gatgtcagtc ctccgtcagt ccgcttacag
    19741 accagtcgat tgttctctcg agagtgttca tatggttggc ggttcgagac gacaataagt
    19801 cgatcaacaa cgatcataaa acctcctctt ggttcattca cgcagcctga cgggagagat
    19861 gaatcatcac accgaggtag tcttgaagct gcctggtgat caagaaccga tgcctggcat
    19921 attccttggc tcgccggccg atcccagcca taagctcaac gtcattgaac atgtgcttaa
    19981 tatagaaggc tgctccttcc ggagaattga cggtatatcc cgttttcccg tgaatgacct
    20041 gggccgtgat ccctcccgcc tccccgccga tcaccggttt gcctttccac atggcctcgg
    20101 tgactgtcag cccgaatccc tcgcgggtgg atttttgcaa cacaatcgcg gcggcccgtt
    20161 gcagcgcatt aatctcgatg ttcgccgtcg gaggaagcag caagagatga acgtccggat
    20221 cgacacccac cgcttcacgc acttcagcca gaacctctgc gctttccgga tcgtcatcgg
    20281 cgctcccgcc cgccagcacc aaccggcagt cgtgactctt ctttacaatc ctgtaggctt
    20341 cgaccacccc aaccggatcc ttgaaacgat cgaagcgtga gatttgcacg agcagcggtt
    20401 tatcccgggg aacttcgagc cggtcgagga tggctccgac ctcctcttcc ggaagttccc
    20461 ggttcttgtc gctgagcgga tcgatcgagg ggtaaatgag aaattgcgga atcggcagcc
    20521 gctgagcaaa tttcggcaac gagaacacag ccgcgtcata gcgaatgatg taagggcgca
    20581 ggaattgcca gacggagcgc tgcggcgtgg acagatcgat atgacagcgc cagatccact
    20641 tcgagccggg ggagcgatgg tcgatgagcg ccgccggctg agggtcatgt atcaaggcga
    20701 catcggcgtc caggttcaat cgtctgccat tctcgcggtt gcattcgagg taggcatcga
    20761 acatctcttt ggtgaggcgt tgcttcgtgc cctgcagcga gttgtggaaa ctcttcgtcg
    20821 tccggtagaa caggtcggtt cctgtgagca cttcccaacg ggccttcaca cccaattcat
    20881 ccatcaacgg aagtaatcgg tggagaattt ctgcgacacc gccaccatag cgggtggaat
    20941 tcacgtgaag gatcgttttc ccctccgcca attcgcccaa ccgtcggagg aaatccactg
    21001 ttccggtcgg agccacatcc tgatactccg ccagtattgg tgccatcccg ctctcctcac
    21061 tcggtaccgt ctcaggcagc tgcgctcaag gcctgatcac agaggttgac gatgcgcgtc
    21121 ctcatgcctt ccaggttcaa tccgaggcgc cccacattga cgacccgcgc acccaactcc
    21181 accaggccca gccctgcttt ggaggacagc caggcggcaa aatccccgct gggtcgacgt
    21241 ttccgcatcc gcgcctcgca gatatggttg tacacggttc caatctccac gcggctcagt
    21301 tcgtcacgaa attcatgaag agtggccacc tcgatgaata agtccacttc gatgacatgc
    21361 gaacggatga actcgaacgg ttcggtgctc cttgtcgaca cggcccccct cgtatggtcc
    21421 aggtgatcgc cgatgatgcc gatcaattcg gcccgcagag tctcgatgtc gtcgaagtcg
    21481 aaggggtcga cgacgcccaa tcgctcaccg agcacgcgat cttgcgcatg tcgtaccacc
    21541 caactcgcaa agtcgttggg atacagttcc tgagtatagg gatgccgcag gtaataactg
    21601 tgggtgtgat aataaatgct gtccgctggc acctcttcga tgatctccag cagccgctcg
    21661 agatcctgcg cccgtttccc gagactctcc cgcaactcca tacacgagac gaaacgaaac
    21721 ggcgagtcgg ctgaacggtc ctccattcag aacctcctct ctgttcgtga gcgatggatc
    21781 atgtcttgtc tgcgtcgcac atcatcggtg gtgcagcctc ttcgacacga gaggcgacgc
    21841 ctcctgccac gcggatatgc ttcggctttg ggtatccttc acgacgtgct catttctccc
    21901 tggaaccagc gcgagccagc tctgcaatcg cctcccactc atggtccgct tcgaccgctt
    21961 caatcagctg tccgaagata tcgggtatcg ccgccgtcac gcgactccag ttcgaaatca
    22021 tcggaactcc gagcggatct tcgaggaatc gctcggtggc gatcttcatt cccgtgagaa
    22081 acacttcaac agctgtccgc tcttcatgtc gatcaaagcc caggccattg atggcggcat
    22141 cgttttcata ccggctgatc gcctcctgcg ccgcctgaag gtaagtggcc ctcagcgtct
    22201 tcaagatgct ttcggaaagg acgacccctt cgttcgcgag attgcgaaac agcgctttcg
    22261 tgatgtcgac acacatcctc agaagaccgg attcgggatc gtccggcgaa agcggttgat
    22321 gtttgtgctc ataggcgtcc gcaatatcga cttggcagac tctacgcggc gcgcagttcc
    22381 tgtacacctc cgcgaggaca ccgacctcca agcccctgtc tcccgggata cgattcgtcc
    22441 aggccagatc gttgaccatg gcgaattccc cggcgagcgg gtaccggaaa ctgtctaaga
    22501 acgtcaggaa tggatggaag ccgaccaggt gctgcaggct gcgcaagacc ggggtcatga
    22561 acaaacgcga cacgcggcca tgcagtcggt tcgtcactcg gctgtaatag ccctttgcga
    22621 actcgtatcc aagattcgga tgagcgatgg gataacagag gcgggcgaga tattgacgat
    22681 catagctcaa gatatcgcag tcatgcagcg cgatcacctt gctttgatgg cgcgccagca
    22741 catatccaaa cgccgtccaa caaccacgcc ccttcccttg cagaccaatg tccatcgcat
    22801 gcgatgtcag caccttcagg atctgctgga tacgagcgcc gtcgttccac acaattcgga
    22861 cgcgttgggg cagcaccgaa aaacattctt tggcttgccg gaattgcaac ggcgtcgctc
    22921 gatccaatga aatgacgatc tcgttcagat agcggatctg agtgagcgtt tcgacgattc
    22981 ccttcaacgc gggtccgctc agttccgaaa acaacatcgg caacaccagc gcgataggat
    23041 tcgtgccggc atgtcgttgg agttcagctt ccagctgctc aagattcggt cgccctaacc
    23101 gatggaggac ggtcacgacg ccattttgat gaaaatcaga cactcgcttc tgctccatcc
    23161 cctctttttt cgagcctcag caccgcgcca tccacagcac acagcagaat catctgcctt
    23221 tcaggatctc ggttgtgatt ccaccccgtg atcctcattg atggctcgtc ttctcgcgga
    23281 gaagtggagt cggatgttgt ggcaacggac ccgttctttt ctttcacgac ccggtttcta
    23341 caaaaccaac actcgaccaa cgccgcctcg ctttcgcggt ggtgctccct accgagagat
    23401 gatggcgcac gatcacgggg tcgacgatgt ctccgcagtt cagacaacgc cagcctttga
    23461 aatccaactc gccgctcacg ttcaggatat ccatgaactg gtccctgacc atatagccct
    23521 gacaacgttg gcatcgcatg ctgtctatcc tccgttagtg tgccagttga ttctgaccgg
    23581 tccattcctc tccagtacgc tctgcgctcc ccgccacggg gctgaatgat cggcagccac
    23641 gccctgccgg atgcgggaac ctcatgaggc cgcatgattg acaagatgct tgccttgccg
    23701 gattaccttt ttgagtgaat ccttcgcgtc acccagaaag tgactcgtct gctcttccag
    23761 gtcttcagcc agattgccaa gctgacgtct cgtcctggcg cctgattgag gcgccaataa
    23821 aataccggtg ccgattcccg ccacgagacc aacgaagaaa gttgtcgtac tcatcaatgt
    23881 gcttcgtgtt ccctcggtca taactctatc ctcctcttgt gcgtattatt gcctgtgctt
    23941 tctcacctca taaggtcgat aaggtcggca ttctcgttcg atttcacttg gcgtcagctc
    24001 tttgggttca cggcaattgc tgcagcgggc cgccagtaaa ccacttcggg tattcccctt
    24061 tacgcgaaac acaaggacgt agcggcgact cccacagttg gggcacatgt gaccttccag
    24121 attgcgcaga accggtacgc ctccgaccgt caggcccctc tgcatgtcgg aatgcatcat
    24181 tgcgtgatcg cgacgtatgt cttccatgat ttattcctcg ttcgaaacgc gatgttgaaa
    24241 gagcgactcc gcagcgtcga ggccagaccc atgatgtcgt gaagaattgc aaaacccgga
    24301 ccgtttcgcc tgatcaccac atcatcatcg gacatctcgc aatgattaca acgaataagg
    24361 gcgcgacagg ccaacaaggt gaggaggatg atcgatcatg aattttgaca cacgatcgga
    24421 ctggttggat tgcactgact gacggatttt atccaagttg cagtcatata aggagattct
    24481 ctgtgcaagg tacagtttat ccgctcggga caaaatgtct tgatgaaaac gtcatcatgt
    24541 ggaaaacacg acggtggcgg ctcgggttac ttcgttacga caatttcgac tcggccatct
    24601 ctccgcagca cattcacgct gacatcatgc gcatgacgtt cgagcgagag gtccacgacc
    24661 gcgttcccga tccggagatt cttgatttca acgcactcca ggtacggcgg cagaaccggc
    24721 tgtgaaaatg agagccgagc ccggggggct tcgatccgca gaccgagaca ggattggagc
    24781 agcagaaaca cggcggccga ggaccatgtc tggggactgc acgcgacggg atacagggtc
    24841 ggactttcac ctggccggcg cgagaaaccg cacaacagtt ctggcaagcg gtgcagctcg
    24901 agaaacaggc tggcgtcgaa gaggccgttg agaatcctca atgcgccttc tttaaatccg
    24961 taggaggcca ttccggctgc gatcaccgca ttgtcgtgcg gccagaccga tccattgtgg
    25021 taggccatcg ggttgtagca gctttcggaa gctgcgacgg ttcggatgcc ccagccgtta
    25081 aaatactctt cgcccattaa ggtcttggcc gttctcatgc catgcctctg gtcggcaatg
    25141 ccggtccaga gacagtggcc ggcatttgag gtcctgactc gacagggccg tttgaatccg
    25201 tccaaggcta aggcatagga cgacaggtcg tggcaccaga agcttcgctc gaactgcgcg
    25261 cggaggttgt ccgcttcttt gaggagacgc ctcgcctgct ccacatggcc cagcgccagc
    25321 gccaatcggg ctgcgcgccg cttggcggca aacacgtacc cttgtacttc gcacaacgcg
    25381 atgggccctt ccgccaaact gccatccgca tgaaagacgg catcctgcga atccttccat
    25441 ccttgatgga cgagccctcc tgtcgatcgg cggcgatact ccacgaagcc atcgtgatcg
    25501 aaatctcctg cctcgtcgat ccactgcaag gccagttcaa cattcggcca gatctgggtg
    25561 atgaacgcca gatctcccgt ccgttcgtaa taggttccgg ccaacttgat gaacaacggc
    25621 gtcgcgtcaa cgctcccata gtagcggcca aacggaatct cgttgagggc cgccatctcc
    25681 cccttacgtg tttcatgcaa tattttgcca atctcggcgt cctgctccgg aactgtctcc
    25741 ctcgcctgag tcgctgccag aaacgccagc acgccccgtg cgatggtcgg gttgacccaa
    25801 agcagttcga aggcggtgat gagcccgtcg cgtccgaacg gcgtgctgaa ccaggggaca
    25861 ccggcatagg ggtacgggcc atggggcgtg tccgacacca tcatatggag atcggccagc
    25921 gaacggttca gccagacgtt gaattgctcg ttcgatgtcg cgaccagaca atcttccgct
    25981 ctggcaacat gaagcgcccg ttccacttct gcgagcgcgt gctcatacgt gggaacgtgc
    26041 gtgttgcgac attcgacttc acaggagatc gtgaggtccc attgggcggc gtctcccgcc
    26101 tgcaaaagcg tgtcgcagtg gatcataccg ggatcgactg tcgtgggggc cggcgcacag
    26161 cggagggtca gccagcgctc cacatgatcc aacccctcat accccagcga catgccgtcc
    26221 ttggatgtga cggtttccaa ccgtcgccct cgccggggcc gcgtcttccc gcggacttcg
    26281 aagagatcgg caaaatcagc atccactgaa aacgacagcc tgaccggcag gggaaggagg
    26341 ctatagttgt ggactcgaag ccgctcaaag cagagacctt gcccgatgaa tcgggtgcgc
    26401 gagagatgaa gtgctccacg cccgatggct gtctgtccgt cgtagctcat gtcaggattt
    26461 gtaagatcaa cgttgagcaa cacgttgtct tccttaaccg tcgaactgag cagcaacggc
    26521 cgttgcccgt tgatggtcag ttcgaaacag gacaaaaagc gggtcccttc gtgatagagg
    26581 ccctgcgacc cagttctgag agaattgatg tcgccgtatt gatcgcacaa gctgaacgtg
    26641 tcgccctgct tcaagacctc gatgtggcta tcggtcagcg acgaggatgc aacgatatgg
    26701 cattgatcat ttcgacaaac gacatcttcc atgaggtcct ctcatctgct ccggactgac
    26761 cgactcaacc agcctccgag ggaggccgtg accagaaagg ttgtgccaat gcctgccact
    26821 cccgttgatt cgccgaaccg ttccgtgacc caaccgaatg cgctgatccc gctgatggtc
    26881 gcaaccatcg cccccgtatt gtagaggccg agaacacgcc ccaccatcac cgctggtgtc
    26941 aattcttgca cgacgcccca cgcaatcgga gtgaaggctc ccatgcctcc cccgatcgcg
    27001 atcataagga gactgaccag aaagagttct ctcgtccaga cgagagcccc gatcgccaac
    27061 gcgctcgcca cgctcgtccc tgcgataatt ctcgtacgct ccgacacatt ccactcggta
    27121 aaccgcaaca gcatcatcga catgagcagc aatcccactc ccaatgagga ccacaggtag
    27181 cctacttcga ccggccccag acccaacagc tttctcgcga agacggggaa caacgtcgtc
    27241 agggcgcctg cgccaaaccc gtaacacccg gcagtcacga tcagcagcag gatggcacgt
    27301 tgtttgatcg cgacaaaggc caagccttcc aggagatcgt gcagaactga accatggctc
    27361 gcctttggtc cagcggatgg gtcccgtact tccagtcgta ccaacccgag acataaggcc
    27421 gaaacgaaat aggtgaccgc gttcagacag agcacttcct gcgaaccgaa cagggcgatt
    27481 ccgatcccgc tcaccaccgg accgaagatg ataccgaggc tggtcgtgct ctggatgagc
    27541 gcatttgctg ctgtgaattg tgcgcgcggg acaatcaggg gcaccgacga cgtgagcgca
    27601 ggactgaaca tcgccatcgc caccgcatcg aggagcacca gaacatacaa cagattgacc
    27661 gtgaaggtgt cggtcgagat ggcacaggga atgatcccga tcagcaccgc ccgcaaggtg
    27721 ttcgatccga taaggaagag ttttttcggc agccggtcca catatacgcc gatgaaggga
    27781 ccgaggatga tgggcggaat cgtatggaga agtccgataa tggtggtttt caccggcgaa
    27841 ccggtaattg aatacacgaa ccataggagg gcaagattcg agataccatc gccaacttgg
    27901 gataccagtt ggctgaacca aaccaatcca aaatcccgcg caagaagcag gcgccagtga
    27961 ctcacagcac gcgccgctcc tggtgtcgtt ccgttctgtc gcgtcgaggt tcgattgatc
    28021 gtcagggtct gccaagtcct catccctcac tctcgatcct gaccttggcc gtcttcacgg
    28081 cttttacccc gggaacctgc cgcgccgctt ctgcagcttc gaacacaatg acttggaatc
    28141 gcacggtgcc aacgagcgaa accactcccc cctcggtttt cacttcgaag ccggccgcct
    28201 tcaaggtggc actcttggcg aaccgttcct tcacttggtg agtaatgatg tcgtcctgcc
    28261 tgcgcgccaa gacttccgga agttctttca gaatctcaat cttgttagcc acggacttca
    28321 cgcccggcac ggtgcgtgcg atctcggccg cgacggattt ttcagcctcg gtggaaacct
    28381 ttcccgacaa gacgattccg tccgatccgg cctccacctc gatctcataa ggaaagaggc
    28441 gggaatcttc cagcagggcg agtttgaccg tgaggatcag cgaggagacc gctttcttgc
    28501 cggtaccctc tttctctttt tctttgtctt tttctttgtc tttgtctttc tctttgtctt
    28561 tgtctgtcac ctcttcgcca ttgtcttttt tcaccttcga ttcgtcactc ttggatgcct
    28621 gcgtcttcgg ctgagccggt ttcgactcct gagcccacag gatcgaagac tgatcaatct
    28681 gtgcgagcat cggattcgtg acctgactca cgaccaatgc acacacacag aaaaccagga
    28741 gcagcgcact ctgctcaatg ccggtgcgcc ggaacagggg acgtttatct ttcatttcca
    28801 tcgagatcag cccaggcgat gaacaacgat ggaaccgctt tttacgctgg caggagatcc
    28861 ggagccgtcg tgccagggtg aaggtgtcgt tgactgaagg cagccgcaac ggtcgcggtg
    28921 cccaagaaca ccactccgat accgacaaga ctcacggccg gaccgaggac atccgcggcc
    28981 caaccaaacc ctgccatccc ggccatcgcg gaagccatgg caccggtgct aaacgtggtg
    29041 aagacgcggc ccagcaggtg ttcaggcgtc agttcttgca gcatcgccca tacgaccggc
    29101 gtaaatacgg ctgtgctgcc gccgaacacg acgatgaggg cagccgccat caccggagcc
    29161 tccagcatac cgagcgtgca caccgcgacg gcaccgacgg ctaaagcagc cgaaatgagc
    29221 cgcaagcggt ttcggatatc tccctgcttc acgccggcca gccagacaga tgcggccaac
    29281 atgcccactc caagcgacga ccagagccac ccgagttcga cgggagttgc gtcgaggagt
    29341 tgcgtcgcaa acacgggcag cagaaacacg aaggcgcttg cgcccaagct atacaacgtg
    29401 gcggtgagca tgaggagcag tacggtccga tgctggataa agacaaatcg gaatccgacg
    29461 aggaggtctt ggacgaggcc tgttctgtca gcctgccatg ctgttctccg cggactctcg
    29521 tgcaggtgga tcggcatgag gcacaaggcg gagaccagga acgtagccgc atcgaggtac
    29581 aggacgtttt gggctccgat catggcgata ccgagaccgc cgaccgctgg accgatcagg
    29641 agaccaatat tcgtcgtact ttggatcagc gcattagccg ccgtgagttg tgcgcgcgac
    29701 acgatgagcg gaacgctgga cgccaacgcc ggaccgaaga tcgtagacac gatcgacgtc
    29761 acaaacacca ggacatacag ccgttcgagc gtcaggatgt ccagcgcata caagagcggg
    29821 atgagcagga ccagtacggt ccggatgaga tccactccga tcatcaccgg tttgcgtctt
    29881 acccgatcga gatacacacc gatgagcgga ccgaacacca gcggaggaat cgtttgcagc
    29941 aatccaat
//
LOCUS       DATIFR010000083         1647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2350047, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000083 DATIFR010000000
VERSION     DATIFR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350047"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1570)
                     /locus_tag="VLD60_04645"
     CDS             complement(8..1570)
                     /locus_tag="VLD60_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB44277.1"
                     /translation="MKPRPTKDHWRGRWLIAGVWLLSASPSVYAAEEMKAQPQERREA
                     ISLADAALRALKNNLDISISRQTKESRLTDIIVEQAKFDPTLSLNGQYNRQVNPLNRP
                     VFGGTGFNLNTIQTFDQRSTSVTVDAQTNLITGGNIDLNYSPARTNVNQDVASGFLFN
                     PAYTGGLALTLTQPLLKNAGIEVNKTFIKIAQNNATVEEHVFRDRVLTVLATVEQTYW
                     EVVFANENLKVAEAALKAAQELLATNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAE
                     KAIRDQEDQLRRLLNPGEEELRLEVRLTPSDRPVVALEPISLQEAIDIAIEQRPEIVQ
                     AKKNLETSEINTKFAKNQILPTLSFQGTMGLAGLGSDYSNSVNNNFSGDFYNYGAGLV
                     LSYPLGNRSAWSTFNRRQMEGKNAEASLVSVRQQIIIGVKEAVRRVQTDFKRIETTRS
                     ARIMAEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAVVDYNKSLSNLARHE
                     ATTLNHYNLQLQ"
BASE COUNT          296 a          450 c          485 g          416 t
ORIGIN      
        1 tggatggtta ttgtaattgg agattgtagt ggttgagagt ggtggcttcg tggcgagcca
       61 ggttggagag ggacttgttg tagtccacca ctgcccggag ttcgttcccc tgcgccgtcg
      121 ccaggtcgcg ttggaaatcc agcacgaatc gcgtcgtgct cagcccgacc ttcagccgtt
      181 cctgttcggc ctggagctgc ttctccgcca tgatgcgcgc cgaccgggtc gtttcgatac
      241 gtttgaaatc ggtctgcacc cgccggacgg cttctttgac cccgatgatg atctgctgcc
      301 ggacgctgac aagtgaggct tctgcgttct ttccttccat ctgacggcgg ttgaatgtgc
      361 tccaggcgga ccggttgccg agcgggtagc tcaggacgag gcccgcaccg taattgtaaa
      421 aatcgccact gaaattgttg ttgacagagt tggagtagtc gctacccagg ccggccagac
      481 ccatggttcc ttgaaaggag agggtcggca gaatctgatt cttagcgaat ttcgtgttga
      541 tttcgcttgt ttccaggttt ttcttcgcct ggacgatttc cggcctctgc tcgatggcga
      601 tatcgatcgc ttcctgcaag ctgatcggtt ccaaggcgac gaccggccta tcggacggag
      661 tgagccgtac ctcaagtcgc aattcctctt caccgggatt taagagccgg cggagttgat
      721 cttcctgatc gcgaatcgct ttctccgcga caagtatctg ctcgacgcgt gaggcgacgg
      781 cggcttcggc ctgaaggacg tcgacgatcg acatgacacc agccttggcc ttggcccggt
      841 tggtggccag caactcctgc gcggctttca gagccgcttc cgcgaccttc aaattctcgt
      901 ttgcgaagac gacttcccag taggtctgct cgaccgtcgc cagcacggtc aggacacggt
      961 cgcggaagac gtgctcttcc acggtcgcat tgttctgggc gatcttgatg aaggttttat
     1021 ttacttcgat cccggcgttc ttcaacagcg gctgcgtgag cgttaaggcg aggccgcctg
     1081 tgtaggctgg gttgaacaag aatcccgagg cgacgtcctg attcacgttc gttcgagcag
     1141 gactgtagtt gaggtcgata ttcccgccag tgatcagatt ggtctgtgca tccaccgtga
     1201 cggaggtgct ccgctggtcg aatgtctgga tagtgtttaa gttaaagcca gtgccaccga
     1261 aaaccggccg gttcaacgga ttcacttgac gattgtactg tccgttaagg ctgagggtcg
     1321 ggtcgaactt ggcctgttca acgatgatat ccgtcaaccg gctttccttg gtctgccggc
     1381 taatgctgat atccaagttg ttcttgagcg cccgaagtgc ggcatcggcc agggagatcg
     1441 cttcccgtcg ctcctgaggc tgcgccttca tctcttctgc cgcatagaca gatggggaag
     1501 ccgagaggag ccagacgccg gcgatgagcc accttccgcg ccagtggtct ttcgtaggtc
     1561 gaggtttcat aatgcccccc tttggtcgac aaaggttcgt tggccatact ataatcattt
     1621 catgcgtggc tgtcatgaat ttcctca
//
LOCUS       DATIFR010000084        10191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1392263, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000084 DATIFR010000000
VERSION     DATIFR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392263"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..946
                     /locus_tag="VLD60_04650"
     CDS             122..946
                     /locus_tag="VLD60_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HSB44278.1"
                     /translation="MMELLTYDFMQRSLLAAAMVGGLCSVIGVFVVLRGLAFVGAGTA
                     HAAFAGVALGFLIGWPPLLLAILFGLATVWITGWVEEKGTMKLDVSIGILYTATMALA
                     ILFIGLMKTYNAEVYGYLFGNVLSVTTEELRTIGGLAVLVLGTLLLFSKELYFIAFDQ
                     EMAEASGVPARRIFFLLLTLVALTVVISLKTVGAILVFAMILIPASTAYQLTHSLSTL
                     TWYSVFIGVCCSVGGVLLSAFWDIPSGPAIVLLSTLVFFVSVLLSPKHLRQRLAAH"
     gene            1362..2006
                     /locus_tag="VLD60_04655"
     CDS             1362..2006
                     /locus_tag="VLD60_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB44279.1"
                     /translation="MGTVTYRGWGWMSLLLVGALLLGVVPDQAQAEMQNGGIEPGKFV
                     LGMRAGFAPVTQTVSAGTSTDVGSLVNFEAMYSLNNWLLVGMMLEWERHSMSQNNPSL
                     DLGHQDTVSILPTVELRPVRFGSLTPYVNMSFGVNVNSFGESSAISGNTISPSNTFAW
                     RLGWGADYMLTNQLALNTEMAYKRNDGHVTVNGVQNNDWNASSFGFLFGVKMFF"
     gene            complement(2180..2611)
                     /locus_tag="VLD60_04660"
     CDS             complement(2180..2611)
                     /locus_tag="VLD60_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB44280.1"
                     /translation="MPPTLKPAVTFSEKKDLEASQLLRLFHQAPWAKDRTLEDAKEML
                     RHTDVALCAWDGDQLVGFGRVLTDFVYRASIWDVIVDKAYHKQGIGTEIVQRILNHPR
                     LRKVELFWLCTRRPAFYEKLGFSSKEQTGMVWSRSKHGRLE"
     gene            2673..2852
                     /locus_tag="VLD60_04665"
     CDS             2673..2852
                     /locus_tag="VLD60_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44281.1"
                     /translation="MSTQVRSCPKCFQLMWLKQDEYVLLDEETVRAKCPHCGSTVRFK
                     LVSEGANAAGPKMGH"
     gene            complement(2962..3384)
                     /locus_tag="VLD60_04670"
     CDS             complement(2962..3384)
                     /locus_tag="VLD60_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44282.1"
                     /translation="MQTGDMLDAIGGLTSQLKTYAAKLPPIVHLTAVPTGVKPKLEAV
                     DDYEDIVSRVRSQSAGTAYKGLNESFVASLEAFEVGNLLGAVQPLLAVLDHIERMQRD
                     KEIEVGRIDEKRLAEYRAALHKILPGNKPELDGAGRGM"
     gene            complement(3417..4382)
                     /locus_tag="VLD60_04675"
     CDS             complement(3417..4382)
                     /locus_tag="VLD60_04675"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSB44283.1"
                     /translation="MITEFIVTNGEQPKRLDVFLVNRERDISRAAIQRLIEQGRVKIN
                     GHVVKPSQKIKPGDVIAFDIPKAEPLTLKGEAIPLEILFEDDALLVLNKPSGLVVHPA
                     PGHWSGTLVNALLHHFATSGGTVSTIGGKERPGLVHRLDKDTSGVMVIAKTDQAHRQL
                     AGQFKHHTITRVYEALIWAVPKKGHGLIELAIGRDTKERKKFSARTTNPKESVTEYKV
                     DHRYGKVAAHVLLYPRTGRTHQLRVHLTSLGHPILGDLTYGGRKVCTMDEVPIPRVML
                     HARTLGFHHPVTGQFLEYTRPSPPDMEAVMQALDRLAHPKTVRSA"
     gene            4509..5096
                     /locus_tag="VLD60_04680"
     CDS             4509..5096
                     /locus_tag="VLD60_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44284.1"
                     /translation="MMGRTAIGVRSTMAMALSLGLSVSSATATDLPAIPPETVADYIH
                     SVIEADRTFYTIHVVERLQKHGKTAAAENWRAKKNVLPLPAQFLMESSELATKTGSRV
                     RYRLISLWPINPHNAPYSESEKSGLDVVREHPEKSVTGTIKVGSQTYFQAIYADRAVS
                     QACVGCHNAHPRSSKKDFKINDVMGGLVIEIPLDR"
     gene            complement(5186..6064)
                     /locus_tag="VLD60_04685"
     CDS             complement(5186..6064)
                     /locus_tag="VLD60_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44285.1"
                     /translation="MPKRILLLTMLLSCIGCAGPALTSRVVQDETSWFVRLDSYKDPG
                     NSSVRYEHPATWTTEDVLAILSRLLLEERGGLMDSARPPHSVFSAEEVTLLVPAIQDS
                     FRMAKPSEWVAFSISTPSDSGLAVTSGGMFLADSRLHVIVANHRALVAQNSEELASVR
                     ANPFHSIRGSGGVLTFESSRFVIGRQSNWSGGHRASASELILDHRGFLSFLKLAGTTP
                     VPAQAAGSSGPAGSSESGASHTRSSTNGDSDPNRSLIRLQEEVERLKKQVAEQAAEIE
                     RLNRRLDHSPRTPPGP"
     gene            complement(6179..6601)
                     /locus_tag="VLD60_04690"
     CDS             complement(6179..6601)
                     /locus_tag="VLD60_04690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HSB44286.1"
                     /translation="MAGKILLEVVTPEKLVLSQQVDELIAPGVEGEFGVLPGHTYFLS
                     MLKIGELRYRVGDQTIYMSVLWGFAEVTPKKVTILAEIAEKAEDIDVDRATQKVAEAE
                     ERLKVGGLPSDLKEAQISLEKARLRKRIAERARKTGLA"
     gene            complement(6771..8219)
                     /gene="atpD"
                     /locus_tag="VLD60_04695"
     CDS             complement(6771..8219)
                     /gene="atpD"
                     /locus_tag="VLD60_04695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSB44287.1"
                     /translation="MATAIGKVIQVIGPAVDVEFPPGQLPNIYNALTVNQAENKAAGK
                     PAIRITLEVAQHLGENRVRSVAMSTTDGLTRGMDVQDTGAPISVPVGRETLGRLINVL
                     GEPVDEKGAIKTKKTYPIHRPAPKLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFG
                     GAGVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDFTKS
                     KAALVYGQMNEPPGARLRVGLTGLTVAEYFRDEENQDVLLFIDNIFRFTQAGSEVSAL
                     LGRMPSAVGYQPTLSTEMGALQERITSTKRGSITSVQAVYVPADDLTDPAPATAFAHL
                     DATTVLSRQQAELGIYPAVDPLDSTSRILDPQILGEEHYKVARGVQSILQRYKDLQDI
                     IAILGMDELSEDDKMVVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFKEIL
                     DGKYDHLPEQAFYMVGPIEEAVAKAEKMGIKV"
     gene            complement(8312..9184)
                     /gene="atpG"
                     /locus_tag="VLD60_04700"
     CDS             complement(8312..9184)
                     /gene="atpG"
                     /locus_tag="VLD60_04700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSB44288.1"
                     /translation="MPSLQSLRRKIASVKNTQKITKAMKMVASAKLKRAQGRILAFRP
                     YGLKMREVVANLSRRVNRDLHPLLQKRPTKMVRVVVVTSDRGLCGAFNANIVRRALEF
                     IRECEARGVKVEIGLVGRKGRDFFKRRHWPTHEPYIDIFDRLSYEHGMEIAQRALEMY
                     TQGAFDEAHIIYNEFKSALQQRVIVEQLFPIESLEEGGQPSETLGGSYLYEPDELELL
                     DPLLMKHLQAQTYRILLESSAAEQAARMAAMDGATRNAGEVIKKVTLYYNKTRQAAIT
                     KELMDIVGGAEALK"
     gene            complement(9246..>10191)
                     /gene="atpA"
                     /locus_tag="VLD60_04705"
     CDS             complement(9246..>10191)
                     /gene="atpA"
                     /locus_tag="VLD60_04705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSB44289.1"
                     /translation="FCIYVAIGQKRSTVARVVKTLEEHHAMEFSVVVAATASDPAPMQ
                     YLAPFAGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLLLRRPPGREAYPGDVFYL
                     HSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIYLGSDLFYS
                     GVRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREMAAFAQFGSELDKATQRQ
                     LARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVQVDKIQQFEADLLRYVAQN
                     HPELKKEITSIAKIDDKVSSRLQEILTAFKQKMGYGAK"
BASE COUNT         2055 a         2918 c         2865 g         2353 t
ORIGIN      
        1 agccgacgtc ctgaagcccg atatcctgag ccaagtctat gggaaagacc tggtcatcac
       61 ggaaaaggat ctgatcctcg tcgaggacta ccaccatcat cactaagcag cataaatctt
      121 catgatggaa ctgctcacct acgactttat gcaacggtcc ctcctcgctg cggcgatggt
      181 gggggggctc tgctccgtga tcggcgtgtt tgtcgtgctg cgaggactcg cgtttgtcgg
      241 agcggggacc gcgcatgccg ccttcgccgg ggtggcattg ggctttctga tcggctggcc
      301 accgcttctc ctagcgattc tgttcggact cgcgacggtc tggattacgg gatgggtgga
      361 ggagaaggga actatgaagc tcgatgtgtc gatcgggatt ctttacaccg cgactatggc
      421 attggccatt ctgttcatcg gcctgatgaa aacatacaat gccgaggtgt acggctattt
      481 gtttggaaat gttttgtcag tgacgacgga ggagctgcga acaatcggcg ggctcgccgt
      541 tctggtgctc ggcacgttgc tgctgttctc caaagagctg tacttcattg cgttcgacca
      601 ggaaatggca gaagcctccg gtgtgccggc cagacggata ttctttctcc tcttgactct
      661 tgttgccctg accgtcgtga tctcactgaa gacggtcggt gcgattttgg tattcgcgat
      721 gatcctcatc ccggcatcga cggcctacca gctcactcat agcctctcaa ccctcacgtg
      781 gtattcggtg ttcatcggcg tctgctgttc ggttggtgga gtcctcctgt cggcattttg
      841 ggacattccc tccggtcccg caatcgtcct tctctcaaca ttggtattct tcgtctcagt
      901 cctcttgtcg ccgaaacatt tacgccaacg acttgctgct cactgattga gcgcaacggg
      961 agctgatcga aacttccgtt gatcggttct tccggatttt cacttttgct ttatgctatt
     1021 ccacatgact ttgtttccca ctagtggtaa gaattatctc tagatagcgg cggcaacggt
     1081 ggtttggcta tcaagagcca tgctatagcc gtccacttct gtccgggcaa agatgagtag
     1141 ggttggggat acagtgctgt ggcacagagg agtcactttt tggctttttg tagacgatga
     1201 caacagcttc acaggtcgtc tagagccaga aaggtgtaat tcgacagtta actgaggctg
     1261 gtcttccgca acatgcacca gcctccagcg gtatcaatct tgccctacaa agctgccagt
     1321 gcatgtttct gtggtctatt tacatcaaga ggagggttgg tatgggtact gtgacatatc
     1381 gcggttgggg atggatgagc ctcctgctgg tcggggcgct cctgcttggc gtagtgccgg
     1441 atcaggcgca ggctgagatg caaaacggcg gtatcgaacc gggcaagttc gtgctgggga
     1501 tgcgggcagg gtttgcaccg gtgacccaaa ccgtttcagc aggtacttcg actgatgtcg
     1561 gctccctcgt gaattttgaa gcgatgtaca gtctcaacaa ttggctcctc gtcggcatga
     1621 tgttggagtg ggagcggcac agcatgtcac agaataaccc gtctctcgat ctcggtcatc
     1681 aggatactgt gtccatcttg ccgacggtgg aactgcgtcc cgtgcgtttc ggctccctca
     1741 ctccttacgt caatatgagc ttcggcgtga acgtgaacag cttcggggaa agcagtgcga
     1801 ttagcggcaa taccatctcc cccagcaata ccttcgcctg gcgtctgggt tggggagctg
     1861 attacatgct caccaatcaa ttggcgctga acaccgaaat ggcctacaaa cgaaatgacg
     1921 ggcatgtaac agtcaacggc gtccagaaca acgattggaa tgcctcgtca ttcggctttc
     1981 tctttggcgt gaagatgttc ttctagactt ccgggcgtag tctttcccga caaggcaaaa
     2041 ggcaggatgt ctggtcacta gggacatcct gcctttcttt tctgtcctcg ccctaagaca
     2101 tcggctcgat gacctcttta acggcttttc acgctgcggg actatttaga ttggaggaag
     2161 aggtagaagc taaaccgagc tactcaagtc gaccgtgctt gctgcggctc cagaccatcc
     2221 cggtctgttc ctttgagctg aagccgagtt tttcgtaaaa agccggtcgg cgggtgcaga
     2281 gccagaagag ttcgactttt ctgagtcggg ggtgattgag aatccgttga acgatctcgg
     2341 tgccgatccc ttgtttgtgg taagccttgt cgacaatcac atcccaaatg ctcgcacgat
     2401 acacgaaatc cgtcaggaca cgcccgaatc ccaccagctg atccccatcc cacgcgcaga
     2461 gcgccacatc ggtatgacgc agcatctctt tggcatcctc aagcgtgcgg tctttggccc
     2521 agggcgcctg atggaaaagc cgaagcaact gggacgcttc gaggtctttc ttttctgaaa
     2581 aggttactgc gggcttgagg gttggaggca tcttgtacta gattatcttc cgatcaccgc
     2641 gtccatgaca ggcggagtgt acgaggaaaa cgatgtcaac gcaagtccgt agctgcccca
     2701 agtgcttcca gctcatgtgg ctcaagcagg atgagtacgt gctgctcgac gaagagaccg
     2761 ttcgcgccaa gtgtccacac tgcggctcaa ccgttcgctt caagcttgtg tccgaagggg
     2821 cgaacgccgc tgggccgaag atgggacact gatttagacg ctgtcagcat acagcgatca
     2881 gctctcgcta ttaagatctt ctcctctgaa tcccttccct tcaggggctg acagcggatg
     2941 gcggacagct tccccgctca atcacattcc ccgaccggcc ccgtcgagtt ccggtttatt
     3001 tcccggaagt atcttgtgca gcgctgcgcg atattccgca agccgctttt catcgatgcg
     3061 accgacctcg atttctttat cccgctgcat ccgttcgata tggtcgagca ccgccagcag
     3121 cggctggacc gcccccagta agttgccgac ctcaaacgct tcgagcgacg cgacaaacga
     3181 ttcgttgagt cctttgtagg cggtccctgc gctctgactc cgaacgcgcg acacgatatc
     3241 ttcgtagtca tcgacagcct caagcttcgg cttcaccccg gtcggaaccg ccgtcaaatg
     3301 aacgatgggc ggtagcttgg ccgcgtaggt tttgagttga gaggtcaacc ctccgatcgc
     3361 atcaagcata tctcccgttt gcataggacc gtcctaatcg agtaggcgga gggctgtcaa
     3421 gcgctccgga ccgtctttgg atgcgccagt cgatcgagcg cctgcatgac cgcctccata
     3481 tcgggcggag aaggtctcgt atattcaaga aactggccgg tcaccggatg atggaatccc
     3541 aatgtcctgg cgtgcagcat cactcgaggt atgggaactt catccatcgt gcagaccttc
     3601 cgtccgccgt acgtcagatc tccaaggatg ggatggccca aggatgtcag atgcacccgc
     3661 agttggtgtg ttcgtcctgt ccgcgggtac agcaaaacat gagcggcgac cttcccatat
     3721 cgatggtcca ccttgtactc cgtcaccgat tccttcgggt tcgtggtgcg cgctgagaac
     3781 ttcttccgct ctttggtatc ccgtccgatc gccaattcga taaggccatg acccttcttc
     3841 ggcacggccc agatcagggc ttcatagacg cgtgtaatcg tatggtgctt gaattgtccc
     3901 gccagctgtc gatgggcctg atctgtcttg gcgatgacca tgacaccgga cgtgtcctta
     3961 tccaggcgat gaacgagccc cggccgttct ttaccaccga tggtcgaaac cgtccctccg
     4021 gatgttgcaa aatgatgcag caaggcattg accaacgtgc cggaccaatg gccaggcgcc
     4081 gggtggacaa ccaggccgga cggcttgttc agcaccagga gagcatcatc ctcaaacaga
     4141 atctcgagcg gaatcgcctc gcctttcagg gtcagtggct cggccttcgg aatgtcgaag
     4201 gcgatcacgt ccccgggttt gattttctgg ctgggcttca cgacgtggcc gttgatcttc
     4261 acccgacctt gttcgatcag tcgctggata gccgcgcgcg agatatctct ctcgcgattg
     4321 acgagaaaaa catctaaccg tttcggctgt tctccgttcg tcacgataaa ttcggtaatc
     4381 atgtgcggca caaactacta aagagtttca gtcaaatgca atcgaaggat atggagaatt
     4441 tgcctccttg ttgtcggagg attatgctaa ccgtgtgagg ctggtccaca cgaagaaagg
     4501 agcacgcgat gatggggaga acagccatcg gagtccggag taccatggct atggcgctga
     4561 gccttggtct gtctgtcagc tccgctaccg caacggactt gcccgctatt ccaccggaaa
     4621 cggtcgcgga ctacattcat tcggtcatcg aagcggatcg cacgttctac acgatccatg
     4681 tggtcgaacg actgcagaag catgggaaaa ctgctgcggc cgagaattgg cgcgccaaga
     4741 agaacgtcct tccgttgccg gcgcagtttc tcatggagtc cagcgagctc gcgacaaaga
     4801 ccggctcgag ggtccgctat cgactcatca gcctttggcc catcaatcca cacaacgccc
     4861 cgtacagtga atcggaaaaa agcggcctgg atgtggtgcg ggagcatccc gagaagtctg
     4921 tgaccggcac gatcaaggtc ggtagtcaga cctatttcca agccatctac gccgatcgcg
     4981 cggtcagcca ggcctgcgtc ggttgccaca acgcgcatcc gcgaagttcc aagaaagatt
     5041 ttaaaataaa tgacgtgatg ggcggcctgg tgattgaaat tcccctcgat cgctgaagtc
     5101 ccgacccgga accctgttcg cttcgacgcc gggttcgatc cagacaggtg cccggtgtcg
     5161 ggtccggacc gcagggacat gatgctcacg gaccgggggg agtgcgaggg gagtggtcga
     5221 gtcggcggtt cagccgctcg atctccgctg cctgttcggc cacttgcttt ttcagtcgct
     5281 cgacttcttc ctggaggcga atgagcgaac gattgggatc ggaatccccg ttggtcgatg
     5341 aacgcgtatg agaagctccg gactcagagg acccggcagg tcctgatgaa ccagcagcct
     5401 gtgccggaac cggcgttgtg ccggcgagtt tgaggaagga gagaaaccct cgatggtcga
     5461 ggatcagttc gctcgctgac gctcggtgtc cccctgacca attcgactgc ctgcctatca
     5521 caaaacgaga cgattcaaat gtcaggaccc cgccactgcc tctgatagag tggaagggat
     5581 tggcgcgcac gctcgccagt tcctccgaat tctgcgcaac gagcgcgcga tggtttgcca
     5641 cgatcacgtg taaccgagag tcggcgagaa acattccgcc cgacgtgacc gctaatccag
     5701 aatcgctcgg cgttgagatc gagaatgcca cccattcgct cggcttggcc atccgaaaag
     5761 aatcttgtat ggcggggaca aggagggtga cctcctccgc cgaaaacaca gaatgcggcg
     5821 gccgcgcact gtccataaga ccgccccgtt cttcgagcag cagccggctg agaatggcaa
     5881 gcacgtcttc ggtagtccat gtggccggat gttcataacg aacggacgaa ttgccgggat
     5941 ccttgtacga atccagacga acgaaccagg aagtttcgtc ttggactacg cggctcgtca
     6001 gcgccggtcc ggcacaaccg atacacgaga gcagcatagt gagcagaaga attctctttg
     6061 gcatcctgtt ggcgctcaca gaatcggaca ttgccacacg tttcagtctg atagaagctg
     6121 aacagatcgt tccgagaggc ggcagcaagg gactaagcgg agcgacgcgg agcgggactt
     6181 acgccaggcc ggtcttgcgg gcgcgttcgg cgatcctctt gcggaggcgg gctttttcga
     6241 ggctgatctg agcttcttta agatcggacg gtaggccgcc cactttaagg cgttcctcgg
     6301 cctcggccac tttctgcgtg gctcgatcga cgtcaatatc ctcggctttc tcggcgatct
     6361 cggccaggat tgtcaccttc ttcggcgtga cctcggcaaa tccccagagg acggacatgt
     6421 agatggtctg atcgccgaca cgatagcgca gctcgccgat tttgagcatg gaaagaaagt
     6481 atgtgtggcc gggcaggacg ccaaactcac cctctacgcc gggcgcaatg agctcatcca
     6541 cctgctggct cagtaccaac ttctccggcg tcaccacttc taacagaatc ttcccagcca
     6601 ttctttcctt cttcctactt tccaaacaga agggggaaaa gtaggcgggc tgctctgacc
     6661 tgcgcgcctt attctcaccc gcccaccccg agcgcgccga gacgcgctct gacccaggcg
     6721 agcaccttct gaggccgcgc gttccgcgag cagaagagca cccgcctgct ttagaccttt
     6781 atccccatct tttcagcctt cgccaccgct tcctcgatcg gccccaccat gtaaaaagcc
     6841 tgttccggca ggtggtcata cttgccgtcg aggatctcct tgaaactccg gacggtgtcc
     6901 ttgagcttca cgtacttgcc tggagcacct gtaaaggctt ctgcaacgtg gaacggctgt
     6961 gagagaaacc gctgaatctt tcgagcacgg gccacgacca tcttgtcgtc ttccgagagt
     7021 tcgtccattc cgagaatcgc gataatgtcc tgcaggtctt tgtaccgttg caggatcgac
     7081 tgcacaccgc gggccacctt ataatgctcc tcgccgagga tctgcggatc gagaatacgc
     7141 gaggtggagt cgagagggtc aaccgccggg tagatgccca gctctgcttg ttgccgtgac
     7201 aacaccgtgg tcgcgtccaa atgtgcgaag gcggttgccg gcgccggatc ggtcaagtcg
     7261 tcggccggca catagacggc ctgcaccgag gtgatcgatc cgcgcttggt cgaagtgatc
     7321 cgttcttgca aggcacccat ttcagttgag agggtgggct gatagccgac tgcggacggc
     7381 atgcgtccga gcaatgcgga cacttccgaa ccggcttggg taaaccggaa gatgttgtcg
     7441 atgaagagca gcacgtcctg attctcttcg tcgcggaaat attcagccac tgtgaggccg
     7501 gtgagaccga cacggagacg cgcaccgggc ggctcattca tctggccata cacgagtgcc
     7561 gctttcgatt tcgtaaaatc atccggatcg atgaccttgg actcctgcat ttcgtgccag
     7621 agatcgttgc cttcacgggt ccgttcgcct acgccagcga atactgagaa acctccgtgg
     7681 tggagagcaa tgttgttgat gagttccatg atgatgacgg tcttgccgac tccggccccg
     7741 ccgaagagtc cgaccttgcc gcctttgcta tagggctcga gcaggtcgac gactttgatc
     7801 ccggtctcga gcacttctgt cttggtatcc tgatcttcca gttttggagc gggccggtga
     7861 ataggatagg tcttctttgt cttgatcgca cccttttcat cgaccggttc gccgagcacg
     7921 ttgataaggc gtccgagcgt ttcgcggccg accggcacgg agatcggtgc cccggtatcc
     7981 tgcacatcca tcccgcgtgt gagaccatcg gtcgtggaca tggccacgct gcggacccgg
     8041 ttttcaccga ggtgttgggc gacctcgagg gtaatccgga tggctggttt gcctgcagcc
     8101 ttgttctccg cctgattcac cgtgagcgca ttatagatgt tgggcagctg gccgggggga
     8161 aactccacgt ctaccgccgg tccgatgact tgaatgactt tgcctattgc agtcgccacg
     8221 ggatgctcct ttatctagag tgctgagggt ctggcaccga gtgcagagtc gaatgctcga
     8281 cactcagaac gtgcatcttt gtaatttcgt cctatttcaa tgcttcggct ccgcctacga
     8341 tgtccatgag ttcttttgta atcgccgcct gtcgtgtctt attgtagtag agcgtgactt
     8401 tcttgatcac ctcacccgcg ttgcgcgtcg caccatccat cgcagccatc cgcgcggcct
     8461 gctctgccgc ggacgattca agaagaatcc tgtacgtctg cgcctgcagg tgcttcatca
     8521 acagagggtc cagcagttcc agttcatcag gctcatagag gtagctccca cccaacgtct
     8581 cgctgggttg tccgccctcc tcaagcgatt cgatggggaa gagttgctca acgatcaccc
     8641 gctgctggag cgccgacttg aattcattgt agatgatatg ggcttcgtcg aacgctccct
     8701 gcgtatacat ctcaagggct ctctgcgcga tctccatccc atgctcgtag gacaatcggt
     8761 caaagatgtc tatgtacggc tcgtgcgtcg gccagtgccg gcgcttgaaa aagtcccgcc
     8821 ccttacgccc cacgaggccg atttccactt tcacgccgcg agcctcgcac tcacggatga
     8881 attccagcgc ccggcgaacg atgttggcat taaatgcgcc gcacaatccc cggtcgcttg
     8941 tcacgacgac gacacgcacc atctttgtcg ggcgtttctg tagtagagga tgcagatcgc
     9001 gattcacgcg gcgactgagg ttggccacca cctcacgcat ctttagcccg tagggccgaa
     9061 aggctaggat gcggccctgg gcacgcttga gctttgcgga ggccaccatc ttcatggcct
     9121 tcgtaatctt ctgcgtattc ttaacgctgg cgatcttccg tcttaggctt tgcaggcttg
     9181 gcatggtttg aattctctgc tctcagcttc agccatagct tgccgctgac agcgaaggct
     9241 gacggctact tcgcgccgta tcccatcttc tgtttgaagg ccgtaagaat ctcctgcaat
     9301 ctgctgctga ccttgtcgtc aatctttgcg atgctggtga tctctttctt cagttcaggg
     9361 tggttctgcg cgacgtagcg gaggaggtcg gcctcaaatt gctgaatctt gtcgacctgg
     9421 acgtcgtcga gaaacccatt gacgccggca tagattgaaa gcacctgatc ggccacgggc
     9481 atcggcttat attgtccttg cttgagcaac tcgaccatgc ggacgccccg cgcgagctgt
     9541 ctttgcgtcg ccttgtcgag ttcgctgccg aattgggcaa aagctgccat ctcacgatac
     9601 tgggcaaggt ccaaccgcaa tgttccagca acctgtttca tcgttttaat ctgagccgag
     9661 ccaccgacgc gagagacgga cagaccgacg ttaatcgccg gacgaacacc ggaatagaac
     9721 aggtcactgc cgaggtagat ctggccgtcg gtgatggaaa tgacgttcgt cggaatgtag
     9781 gccgacacgt cgccggcctg cgtttcaata ataggaagcg ccgtcaagct gcctccaccg
     9841 agcttgtcgc tcagtttcgc tgcacgttcc aataatcggg aatgcagata gaacacgtcg
     9901 cccgggtagg cttcgcggcc cgggggcctg cgaagcaaca acgacagctg tcgataggcc
     9961 acggcgtgct ttgacaggtc gtcgtagacg atcaacgcgt gtttgccgtt atctcggaag
    10021 tactcgccga tcgcggcacc cgcaaagggg gccaggtact gcatcggagc cggatcactg
    10081 gcggtcgccg ccacgacgac gctgaactcc atggcatggt gttcttcaag tgtcttcacc
    10141 acgcgtgcca cggttgagcg cttctgcccg atcgcgacgt aaatacagaa g
//
LOCUS       DATIFR010000085        23052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_243905, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000085 DATIFR010000000
VERSION     DATIFR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243905"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VLD60_04710"
     CDS             complement(<1..655)
                     /locus_tag="VLD60_04710"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HSB44290.1"
                     /translation="MACSNDGNGGSGANLNQIPAFNSTVLIIAPAGDGTGDVYVGGDF
                     TSYNGIAVTRLVRLNADGTVDPVLVTGVGFNGAVRSIASVGDGTGDLYVGGDFTSYRG
                     IAVAGLVRLNADGTVDPALVTGVGFNGAVRSIASVGDGSGDLYVGGDFSNYRGIAVAG
                     LVRLNADGTVDPVLVTGTGFTDTVHRIAPASDGTGDLYVGGAFTSYNGTSANDLVRLN
                     "
     gene            complement(1318..2094)
                     /locus_tag="VLD60_04715"
     CDS             complement(1318..2094)
                     /locus_tag="VLD60_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB44291.1"
                     /translation="MPKRVRTGLTRADILDRYIERFKQQLIKFRPFLSRKRGNSSLED
                     FDEAAEELISQVFGAASDEAEAYLYAKTGESASIPEEAQESGTHNIERESLHQRRQVL
                     ENCYSDLQLRNRLQAARRGGRDGQKMVRASVEDYMSHDVRSIHRAATIKEAGQLLQKY
                     RVGSLIVDDGSRYIGIVTDSDLSRKAVAKGLDPNTTTVTTCMSKPVVSIEESESLADA
                     VAVMKESGIRHLAVTADRTIIGVLSISDVLRAYEDMGGPR"
     gene            complement(2162..3724)
                     /locus_tag="VLD60_04720"
     CDS             complement(2162..3724)
                     /locus_tag="VLD60_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HSB44292.1"
                     /translation="MNKIEVIKAERDGLAVEDMIAQYAKSGWESISEADVQRLKWYGL
                     FLRNPTPGFFMIRVRIPGGKTGSYQLRALADIAGTYGNGVLDLTTRQQIQLRQIRIED
                     VPVVFARMDEVGLTSLQTGMDNVRGVMSCPVAGMNPDEVSDATQIVRAITEEILGNRD
                     YSNLPRKFNVAVTGCPDNCIHMETQDLALVPAYLDDAHGKRLGYNVLAGGKLGSGGYR
                     IATPLDIFVTEDEALDVSRAIIQIFRDHGSRENRTQARLAFLLDEWGESRFRQEIEMR
                     VGKTLSTAGVDARKPTEKSHVGIYRQKQPGMNYVGLKVLVGRIQHGDLIKVAALAEKY
                     GTGEIRISPAQALIIPNVSDRKIGDLDREPLVKHFAYNPSPVYKGLVSCVGSDYCNMA
                     VIETKGRAVETAKALETRLGSSLKPITMHWSGCPAACGNHLVADVGLLGKKAKIDGKV
                     VDAVDIFVGGRSGPDPKLAIKIMEDVPCDKLPMVLEGLVPYHTREKMHRVRGGGSKKV
                     PAGKEPVISTAA"
     gene            complement(3721..3939)
                     /locus_tag="VLD60_04725"
     CDS             complement(3721..3939)
                     /locus_tag="VLD60_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HSB44293.1"
                     /translation="MATTFDRVLTSNEDRVIQRLRECGASSFEDLTRLPGLDWATVFS
                     IVDRLNRAGFVVLERHGADYRVSLEKGT"
     gene            complement(3970..4797)
                     /locus_tag="VLD60_04730"
     CDS             complement(3970..4797)
                     /locus_tag="VLD60_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HSB44294.1"
                     /translation="MYTTDHERVALSAEKKYRFLEVSLPRYLVLSALAGVYVGFGIAL
                     IVSIGGPLATAGSPIVKLVMGVSFGIALTLVIFAGSELFTGNNMTGVIGGLSRSLTWA
                     QVAQLNVWSWVGNLVGSLALAWLVVESGVLSKGPQAELVGNVAAMKMSLPAWELFLRG
                     LLCNWLVCLAVWTSGRTTNDAAKIMLIFWCLFAFIGTGFEHSVANQSLLGMALFLPHG
                     HAVTWAGYWYNQFYVVLGNIVGGGLFVGGLYWLASPYTVNRKEVRELPRPIPAREAC"
     gene            complement(5076..5198)
                     /locus_tag="VLD60_04735"
     CDS             complement(5076..5198)
                     /locus_tag="VLD60_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44295.1"
                     /translation="MTQMLVASSLLVGIVGLLWVVVLAIRQDNRCEHNKKVRTF"
     gene            complement(5484..5924)
                     /gene="cynS"
                     /locus_tag="VLD60_04740"
     CDS             complement(5484..5924)
                     /gene="cynS"
                     /locus_tag="VLD60_04740"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HSB44296.1"
                     /translation="MEKEEVRKAIKAKRLAKKVTIAEVAKAVGRNPTFVAAVLQGTHK
                     LPAEEAKKVGELLGLDAELTASLSKFPVRTDFPNTTDPFKYRLMEIIGVYGDSMREQA
                     NEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(5987..6838)
                     /locus_tag="VLD60_04745"
     CDS             complement(5987..6838)
                     /locus_tag="VLD60_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HSB44297.1"
                     /translation="MPYLQIDHVSKSFPSHTGEGAVCIFEDVTIKIDQGEFVTVIGHS
                     GCGKSTLLNIIAGLETASEGGVILEGKEVVGPGLDRMVVFQNFSLMPWMTVFENIALA
                     VRSAYPNWSHAQVAAHVNKYIALVGLKGAETKRPTALSGGMKQRVGLARAFSIEPKVL
                     LLDEPFAQIDALTRGVIQEELIQMWNSSRNTVFMVTHDVDEAILLSDRILLMTNGPSA
                     RVGEIVDVTIPRPRSRESIIEHPHYYKIRNHVIHFLVRHASHVARGEGESGADGGGDA
                     PLVVNFS"
     gene            complement(6947..7834)
                     /locus_tag="VLD60_04750"
     CDS             complement(6947..7834)
                     /locus_tag="VLD60_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSB44298.1"
                     /translation="MARQATGSPWMITVFLVVIFLLGWHIATLREAFDAKGLTEEQLQ
                     MMEFNGDIVHLPDGTYKWNPEKEKVKGIPGPLAVMEKAREELSEAFVKKGTNDHGIAY
                     LVMYTVQRFGAGFLAASLVAVVLGVFLGLNKVLFKAVNPFIQILKPISPLAWMPLLLY
                     TVRDPKWTAILVVFMASLWPTLATTAFGVNGIRKDYLHVAAILQLSWFKRLFTVILPG
                     AAPTIVNGLRISFGSALVAVVPAEMLLGELGVGYLSWIEWNNLDISGVIFAILVVGCV
                     GVILDAGFNKLAGLVTYQE"
     gene            complement(7956..9269)
                     /locus_tag="VLD60_04755"
     CDS             complement(7956..9269)
                     /locus_tag="VLD60_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HSB44299.1"
                     /translation="MTDDRASRTETVIPPDQSDEGYYKASVERAVAAAVLNAADPGRR
                     RFLALLGTATLTALIADVFPLGRLISFAAESGGKPEKKDLSIGFIPITCATPIIMAEP
                     LGFYKKYGLNASVKRAAGWAMIRDWAINGEVDAAHMLTPMPLAISLGAGSVPKPFYMP
                     AVENINGQAITLHIKHKDVKSAADMKGFRFCVPFDYSMHNYLLRYYLAEGGVHPDKDV
                     QIRVVPPPEMVANLKAGNVDGYLAPDPFNQRAIYENVGFIFKLSKEIWDRHPCCAFTV
                     SEEFATKYPNTFNALFRAIVDATHYASDAAHRKEIAAAIAPTNYLNQPVTVLEQVLTG
                     TYADGLGNIRKDPSRIDFDPYPWHSMAIWILTQMKRWGHLKGDVNYKSVAEQVYRAAD
                     CDRIEKELGYPAHQATTMKHVIMGKEFDPSKPEAYVKGFAIHSMA"
     gene            complement(9610..11019)
                     /gene="glnA"
                     /locus_tag="VLD60_04760"
     CDS             complement(9610..11019)
                     /gene="glnA"
                     /locus_tag="VLD60_04760"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSB44300.1"
                     /translation="MNAREVLEFAKKNKVQVVDLKFVDLIGTWQHFTIPTNELTEDLF
                     KDGSGMDGSSIRGWKAINNSDMLVVPDPSTACLDPFTAVPTLSLIGNVVDPISRETYD
                     RDPRYIAQKAEKHLQSTKIGDISYWGPEAEFFIFDHARYDQTSHSGFYYIDSDEGTWN
                     MGQEGVNLGGKIRHKEGYFPVPPTDTQQDIRTEMILEMEKVGIPIEKHHHEVATAGQA
                     EIDIRFDSLVRTADKMLMYKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQSIWKD
                     GKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAFTNPTTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPGANPYLAFSAMLMAGLDGIENKINPGEPA
                     EKDLYDLEAKEAAKIRTMPGSLDESLRNLEKDYQFLLKGGVFSEDLIEAWIGYKRSKE
                     IDPMRLRPHPYEFFLYYDV"
     gene            complement(11071..13761)
                     /gene="glnD"
                     /locus_tag="VLD60_04765"
     CDS             complement(11071..13761)
                     /gene="glnD"
                     /locus_tag="VLD60_04765"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HSB44301.1"
                     /translation="MTITSGQQGAGLFTHEGDAESLGLFLAEQRRVIGERVMGGASGG
                     ETLAAMTDLVDGLIVGRYREAVRHGGEALANAGLRQCCVMALGGYGRRELAPHSDIDL
                     MFLFRPGAEEAAKSFVRAVLHPLWDCGFQVGHSVRTIADSIELAMADVTVRTSMMESR
                     FLAGSPELFEKFHSRYMRSVVVKGTDKFLDYKLEERRREYEKFGETVYLLEPNVKKSK
                     GGLRDLHLLQWAGMARYRAPTIRELSDRGVLSRADSLALTEAREFLWRVRALLHVHAG
                     TAQEILSFDEQVWLAQHFGFQDQPHLLAVEQFMQQYYRHTMGLHERCMRFIDRCKSVP
                     FWQRMAKFLPKPRVDEYFVVQGTALTVAEEARAKVLESPALLLRLFDLARSRQLTIAP
                     ALLEDIHRHVESVSAEAYRTPEVSRIFLKIMAGPGTTATLEAMHRAFLLEKMVPAMQT
                     VRGLMQFNQYHKYTVDEHSLLAVGRAEALANEPTALGEVYRSIKRKDILHLSVLMHDL
                     GKGQEEDHSDVGKRMAEEVAVRLGLDEQDTRTLSFLVHKHLLMAHTAFRRDPNDVKVV
                     LPFAREIGTPEVLKKLLALTAADIAAVGPGVLTKWKESLLVELYVRTMPEVSGERETQ
                     IAPERLNQVAEEVIRHPCLAGQSGVNRAWIEAQLKQFPERYLYGTPPARIAAHVAAVS
                     RLHVGDVLVDSDFNKELSVCEYTVVTHNELTPGIFSKIAGVMAASGLQILDAQILTRA
                     DGIVVDTFQVTDPDYQGEPPADRRHTVGDRIASVLKGWEHVDDLLRRGARLKLTRPMP
                     KAREATEVRIDNETSDGFTIIDVFADDRQGLLYVITNAIFQLGLSIHAARISTRLDQV
                     ADVFYVTDLHGKKLDDHGQVERLRTGIEKAIENFLEAKAA"
     gene            complement(13758..14096)
                     /locus_tag="VLD60_04770"
     CDS             complement(13758..14096)
                     /locus_tag="VLD60_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSB44302.1"
                     /translation="MKLIEAIVKPFKLDEVKDALLEMGVQGMTVSEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVTDAQVPRVIETITRAAKTGSIGDGKIFVRDLSAAVRI
                     RTGETGDTAL"
     gene            complement(14194..15594)
                     /locus_tag="VLD60_04775"
     CDS             complement(14194..15594)
                     /locus_tag="VLD60_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSB44303.1"
                     /translation="MVIDLKRNCKRNSKRQGTPGGMCLTIVAVMLGTLALFIGSVAMA
                     QDAMAQAGASVATPLLKVDTGDTAWVLMSTALVLAMTMPGLALFYGGLVRSKNVLGTI
                     MHSAIILCMVSLIWILFGYSLAFGPDKGGIIGGLEWIGLNGVGSEPHPTYGPTIPHQV
                     FMLFQLMFAAITPALITGAFAERMRFSALLLFAALWSVVIYTPVAHWIWGGGWLGKMG
                     ALDFAGGAVVHISSGVSALICALVMGQRKGYGTEYMAPHNLPFTLLGTGLLWFGWFGF
                     NAGSALGANGVAGSAFLATHTAAATAALAWMWMEWMHRGKPTVLGVASGAVAGLATVT
                     PGAGFVGPFSAIVIGGFAGILCYFAVVWKGRMGYDDSLDVVGIHGVGGIFGILATGLF
                     ASKAINTAGADGLFFGNTAQFGIQAIMVAAVAIFSAVGTWIILRLVDAMVGLRVSPED
                     EATGLDLSQHNERAYS"
     gene            complement(15625..17019)
                     /locus_tag="VLD60_04780"
     CDS             complement(15625..17019)
                     /locus_tag="VLD60_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSB44304.1"
                     /translation="MPSHVSTMRKVLPVLVCMVTLTGATALYAQDAGAPAPAAPPAAA
                     SEATTAPAAPALPKIDTGDTAWLLVSTALVLAMTAPGLALFYGGMVRSKNSLGTIMQS
                     FIILCLISIQWVLWGYSLAFGPDKGHIIGGLEWLGLNGVGLEPNADYGATVPHQAFMI
                     FQMMFAVITPALITGAFAERMKFSSFLVFTLLWATFIYDPLAHWVWAVGGWVRNLGAL
                     DFAGGTVVHISSGISALACAIVLRQRLGYGREHMSPHNLPMTVLGASLLWFGWFGFNA
                     GSAVASGALATSAFVVTNTATASAALAWMIAEWMYRGKPTVLGAASGAVAGLVAITPA
                     SGFVGPMSSIVIGLGAGVFCYLAVLWKSKFGYDDALDVVGIHGVGGIWGALATGLFAS
                     KAINAAGADGLFYGNPAQFGIQAMAVLISVVFAFTGTFIILKLVDGVMGLRVSEEEER
                     MGLDLSQHDERAYS"
     gene            complement(17043..18365)
                     /locus_tag="VLD60_04785"
     CDS             complement(17043..18365)
                     /locus_tag="VLD60_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB44305.1"
                     /translation="MSRRSLIGIGLCALFLLIDGNSVFAQSPAPAPASTPTVQERLDA
                     LEKKVDAPSLWKTLGFKASGFVDLAYTQNFNNPNSDLNQGHIFDTDANSFMPHLAQLM
                     LERPADAGGSAVERAGFRARLNFGADARVTRARTNYQTGTSNDEIDLQEVYAEYILPV
                     GNGLKVQFGKINTLIGYEVINSFENVNFSRSFMFGTGQAFTTTGVRFTYTFNPLVTAS
                     FGVVNGWDNIDDNNRGKTIEYLVALTPHEKFGVSWYGSYGPEQANRQFGDATSGGAPG
                     DPSAKRFANGLIFTLKPTEKDTVVLEPYYVNESNNPARAANPNLKTNARWNGLAGYLI
                     HDFDEQWSARFRGEIFEDAGGSRVCTGTWNNAGGTNTCAGATNTTAAIPVAQTLWEIT
                     PTLQFKPVPSLITRIEFRYDKSDKNVFLYGTRPTNHQETLSFEVMYLF"
     gene            18921..20384
                     /locus_tag="VLD60_04790"
     CDS             18921..20384
                     /locus_tag="VLD60_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSB44306.1"
                     /translation="MEKEKMERITFVMLVGMAGTIFLVDLLSPPGLAWWALYLFPLML
                     AFSLPNRMAPVLFAVIATALISIRAFSSPSTSPTWLTLGPSVLALAVLWGSVALLGHL
                     QKKWRTISAHRDVSQHEEQRRLRHLFGERVKELTALHRTVRLLQDSAKPIEEALSEIV
                     ALLPPAWQYPEITVARITFDGMVFTTPFFVESPWKQTATWVTPDGAKGMIDVMYREER
                     PSEAEGPFLAEERDLIHSLADSLCSYLTRRRAELALREAHERLKALSHQLMEVQENER
                     RRIAHDLHDEIGQALTVVKMNLQTMQRIADTSAIAIPLMDSSAVIDQTLQHVRDLSLD
                     LRPSLLDDLGLAPAVRWYLSRQAERTGWTIDVTVDESLPPPPQPVAIACFRVLQEAVT
                     NIMRHSNASSVSVSLRQHEGDLLLVVRDNGVGFDVRNALDKAASGQSMGLIGMQERIR
                     GMNGSISIESEPGQGTEVRVRIPVSRLSQPIRAEVST"
     gene            20389..21042
                     /locus_tag="VLD60_04795"
     CDS             20389..21042
                     /locus_tag="VLD60_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB44307.1"
                     /translation="MGTIRVLLADDHRLVRAGLRALLQSLAGVQVVAEAGNGYEAIQL
                     TEQHQPDVVIMDIGMEGLNGLEAAARLAKFTPAPRVIILSMHANEEYVRRSLQAGAVG
                     YLLKGAEPAELEFALQAVVRGETYLTPAVSKQVVQDYLQGGGVKADPLQELTPRQREV
                     LQLIAEGHSTKEIASKLNLSIKTVETHRSELMNRLNIHDVAGLVRYAIRTGLVTPDS"
     gene            21250..21648
                     /locus_tag="VLD60_04800"
     CDS             21250..21648
                     /locus_tag="VLD60_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44308.1"
                     /translation="MTRISQDLTRKPLWMLSAMFLLLPMGNVGCSFATDQVLPAQLES
                     RVSSNATADDHLAAALLYQEQAQLAKTEAGKYEQAAASIRAIEDPKGFRRGALMTAAQ
                     EHRRTADEMQQLYASHEAKAQTMMGKQQAQ"
     gene            complement(21802..22209)
                     /locus_tag="VLD60_04805"
     CDS             complement(21802..22209)
                     /locus_tag="VLD60_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44309.1"
                     /translation="MKVRTRMIVGLIGALALVAIGLPALWHQPDVSGPGGLPEALAGG
                     DVMKEAAQEHLDVAKQYDKDAENHEAQAKHYEQKAAAITPLMDTKGFRREGMKMAADS
                     HSTMATEFRFRANVHRLEAELLLEKAMHAEKEK"
     gene            complement(22264..>23052)
                     /locus_tag="VLD60_04810"
     CDS             complement(22264..>23052)
                     /locus_tag="VLD60_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44310.1"
                     /translation="ELAFVQTLATTPRIIEGSVLSPLPTRPTPFTVEDRRVHLERLGM
                     VTIREVACPGSLAIVHAWVADHSESQGLRRYTACIQLYAGAYRVHLIDSRLVSKSSDG
                     LTGLAENRLKSLPSLLPHIGQAFVAHMKVAREVTNSIATESFASDWQTDEKASIAPSP
                     STGKGVPSAVSQSETSGGTSVARRSDQDVVATSPLVCLAPRYQAAAVRTQHGRGKVIQ
                     VLEQGSLMAVDESVDAAYFRVMTTEGLAGWVNRSDVRRLPCPIG"
BASE COUNT         4791 a         7241 c         6100 g         4920 t
ORIGIN      
        1 cattgagccg caccaggtca ttcgccgacg ttccgttgta gctggtgaag gcacctccca
       61 catacagatc gccggtccca tcactcgccg gggcaatcct gtggacagtg tcggtgaatc
      121 ccgtccccgt caccaagacc ggatccactg ttccatcggc attgaggcgc acgaggccag
      181 ccaccgctat tccgcggtag ttggagaagt cgcctcccac gtacagatcg ccgctcccat
      241 cgcccaccga cgcgatgctg cgtaccgctc cattgaaccc gacccctgtc accaaggccg
      301 gatctactgt cccatcggca ttgaggcgca cgaggccagc caccgctatt ccgcggtaac
      361 tggtgaagtc gcctcccaca tacaggtcgc cggtcccatc acccaccgac gcgatgctgc
      421 gcaccgctcc attgaacccg acccctgtca ccaagaccgg atccactgtt ccatcggcat
      481 tgaggcgcac gaggcgagtc accgctattc cgttgtagct ggtgaagtcg cctcccacat
      541 acacatctcc ggttccatca cccgctggcg cgatgatcaa cacggtacta ttaaacgcag
      601 ggatctgatt aagattggct ccgctgccac cgttcccatc attgctacat gccatggcca
      661 cggcagtgaa tgccgcgaca agtccggctc cgcataagcg tgaaatgccc ctgtggccca
      721 tggatgactt gctatacaag gtagggaaga ccctcctgtt gaaacgtaac cccgctgtga
      781 gcacgctccg aacgatcttg cgaaagtcta tttatatcgt ctgtcttgcg tctatccctc
      841 gaacaggggg gcaaaggccc acctcgactg ttgctgtctc gctggcttgg gatcctgtcc
      901 aggatcccag tgtgacggga tactacgtgc atttcggaat gcaatcgccg cgtttggcgg
      961 gatcctgcac gtacgcgcag agtgccttct gcaatctcgc ttccctgtcc aataaattat
     1021 caccaacggt gattgtgagc ggccttgcgt cgaacacaac ctatttcttt gccgtcggtg
     1081 catataacga cggcctcgaa agcagttatt cagctgaagt gtcgactgtg actaaatccg
     1141 tttagggatc aacagagaag aaggggaaaa ggggataggc aacattccaa agagatgtaa
     1201 cgaccggaca caaacgggag gtggagtctt ctctgattgc actgcatgat gagctgataa
     1261 ccagcccatt gttgccgcgc ggcaagaccc gcgcggcaac aagagcattg gtagcttcta
     1321 gcgcggtcct cccatgtctt cgtaggcccg cagcacgtct gagatcgaca ggacgccgat
     1381 gatcgtgcgg tcggcagtca cggccaggtg gcgaatcccg gattctttca tgacggcaac
     1441 cgcgtcggcg agggactcgc tttcttcgat actgaccacc ggcttgctca tgcaggtcgt
     1501 cacggtggtg gtattgggat cgaggccttt ggccacggcc ttgcggctga ggtcggaatc
     1561 ggtcacgatc ccgatgtagc gggagccgtc gtccacgatc agggagccga ccctgtactt
     1621 ctgcagcagc tgtccggctt ctttgattgt cgcagcacgg tgaatgctgc gcacgtcatg
     1681 ggacatgtag tcctctactg aagcccgaac catcttctgc ccgtctcgtc ctccccgcct
     1741 ggcggcctgc aaccggttcc gcagttgcag atcggagtaa cagttttcca ggacctgccg
     1801 tcgctgatga aggctttcgc gttcgatgtt gtgcgtgccg ctctcctgcg cttcctccgg
     1861 gatcgaagcc gactcgccgg tcttcgcgta gagataggct tccgcttcgt cggacgcggc
     1921 gccgaacacc tgactgatca actcctccgc cgcttcgtcg aaatcctcca atgacgagtt
     1981 cccccgcttg cgcgagagga acggccgaaa tttaatcaat tgctgtttga atcgttcgat
     2041 ataacgatcg agaatgtctg cccgtgtcaa acctgtgcga actcgtttcg gcatctggtc
     2101 cctcctgagt tgaggcacga gaataacgca acacatgtcg cacgctcaag aggtgtcact
     2161 gttatgcggc ggtcgaaatc acaggctcct ttcccgccgg aaccttcttg gatcccccgc
     2221 cccgaacgcg atgcatcttc tctcgcgtgt ggtagggaac cagaccttcg agcaccatcg
     2281 gcagtttgtc acaaggcaca tcttccatga ttttgatcgc cagcttggga tcgggccccg
     2341 aacgcccgcc gacaaagatg tccacggcat cgaccacctt gccatcgatc ttcgccttct
     2401 tccccaacag cccgacatcg gcgaccaaat gattgccgca ggcggcggga caaccggacc
     2461 aatgcatggt aatcggcttg agcgagctgc caaggcgcgt ctctaaggcc ttggcggtct
     2521 ccaccgcccg tcccttggtt tcgatcaccg ccatattgca gtagtcgctc cccacgcaac
     2581 tcaccaaccc cttgtacacc ggcgaggggt tgtaggcgaa gtgcttcacc aacggctccc
     2641 ggtcgaggtc gccgatcttt cgatcggaca cgttgggaat gatcagggcc tgcgccgggg
     2701 aaatccgaat ctcccccgtc ccgtactttt ctgccaacgc ggcaaccttg atcagatcgc
     2761 catgttgaat acggcccacc aggactttga gccctacata gttcatgcct ggctgttttt
     2821 ggcgatagat gccgacatgg ctcttctcag tcggctttcg cgcgtcgaca ccggcggtcg
     2881 acaacgtctt cccgactcgc atttcgattt cctgccggaa gcggctttcg ccccactcat
     2941 ccagcaggaa ggccaaacgc gcttgagtgc ggttctcgcg acttccatga tcccgaaaga
     3001 tttgaatgat ggcacgggac acgtccaagg cctcgtcctc ggtgacaaag atgtcgagcg
     3061 gcgtggcaat ccgatacccg ccggaaccaa gtttgcctcc ggctagcacg ttgtacccga
     3121 ggcgcttgcc gtgagcatca tcgagataag ccggcacgag ggccagatcc tgggtttcca
     3181 tgtgaataca attgtccgga caaccagtga ccgcaacgtt gaacttcctc ggcaaattgg
     3241 aatagtcgcg gttgccgaga atctcctcgg tgatcgcccg gacgatctgc gtcgcatccg
     3301 agacttcatc cgggttcatg cccgcgaccg ggcagctcat gacgccccgc acgttgtcca
     3361 tgccggtttg aagagaagtc agccccacct catccatcct ggcaaataca accgggacgt
     3421 cttcgatccg gatctgccgg agttgtattt gttgccgcgt cgtgaggtcc aggacgccgt
     3481 tgccatacgt cccggcgatg tcggccagag cccgcagctg atatgaaccg gtcttgccgc
     3541 ccggtatccg gacacggatc atgaagaagc ccggtgtggg attccgcaga aagaggccat
     3601 accatttgag gcgctggaca tcggcttcgg aaatggattc ccagccgctt ttggcatact
     3661 gggcgatcat gtcttccacc gccaacccat cacgctccgc cttgatgacc tcgatcttgt
     3721 tcatgttcct ttctccaatg acacacgata atcggccccg tgtctttcaa gcaccacgaa
     3781 gccggcacga ttcagacggt cgacgattga aaaaaccgtg gcccagtcaa ggcccggcaa
     3841 acgagtcagg tcctcaaacg aactcgcacc acactcgcgc agccgttgaa tcacacgatc
     3901 ctcgttcgac gtcagtacac gatcaaaggt ggtcgccatg gttccctcct tcctattcgt
     3961 caccggggat tagcaggcct cccgcgccgg aatgggacgc ggcagttccc ttacctcctt
     4021 ccggttgacg gtatacggac ttgccagcca gtacagacct ccgacgaaca gcccgccgcc
     4081 gacgatgttt cccagtacga catagaactg gttgtaccag tagcctgccc aggtgacggc
     4141 gtgcccgtgg ggcagaaaca gcgccatgcc gaggagcgac tgattcgcga cactgtgctc
     4201 aaatccggtg ccaatgaaag caaagaggca ccagaagatc agcatgatct tggccgcatc
     4261 gttcgtcgtc cgcccggagg tccagaccgc aaggcagacg agccagttgc acagaagacc
     4321 ccggagaaac agctcccacg ccggcaggga cattttcatc gccgccacgt ttccgaccag
     4381 ctccgcctgc ggccccttcg acaggacgcc cgattcgacg accagccagg ccagcgccag
     4441 cgagccgacg agattgccga cccacgacca tacattgagc tgcgcaactt gggcccaggt
     4501 gagactgcgc gacagcccgc cgatgacacc ggtcatatta ttgccggtga ataattccga
     4561 cccggcaaag atcaccagag tgagggcgat cccgaacgat acgcccatca ccagcttcac
     4621 gatcggcgat ccggccgtgg ccagcggccc gccgatgctg acaatcaacg cgatgccgaa
     4681 tcccacatag acgccagcca gagccgagag cacaagatag cgtggcagcg agacttccaa
     4741 gaaccgatat tttttctcgg ccgacaatgc aacccgctcg tgatctgttg tgtacatcac
     4801 cccctccttc atcgtcacca ggttccaaaa acaaagcggc gtccgtctcc caattaagaa
     4861 gacggacgcc gttgtccgta ctgcttcgtt ctctcttagt acgcagcggg aacgccgttg
     4921 ttccccaaac cgacacaata ccctgccaca ttctctgacc ttgagcacca atcccgcaac
     4981 gagtccgacc acccggcgac aactcccctt cttagacaat gatgctgtct cgctgaggag
     5041 cgacgttcag gacatccaat gccaggcaga agcaatcaga aggtacggac cttcttgttg
     5101 tgctcacaac gattgtcttg acggatggcc aggaccacca cccagaggag cccgacgatt
     5161 ccaaccagca agctcgacgc caccagcatt tgagtcatca gaacgctcct ttctgttgat
     5221 tttgctcgac catcgttctg tgttcggttg tcgccacgac ctattacacc ccgacagata
     5281 agcggctcct tttatgggta ttgcctggtc cctccccttc ctgaaatgcc tgactcgacc
     5341 ctgctgctta ggcgcaaaac aaaatagcgt ccgccactct ttcagaatga cggacgccat
     5401 tgtccgcttt gcatcactct ctatttatct tgctgcggaa acgcctttgt tcccgcactc
     5461 gacaacatgc tcttcgactg aacctagaag gtcttatact cgagccactt cccgttcagt
     5521 gtaattttgc agcgggcctc gccctggctg cctgtcacct tttccatgtc taacgtaaag
     5581 tcgatcgcgc tcatgatccc gtctccgaac atctcgttcg cctgctcccg catcgagtct
     5641 ccatagacgc cgatgatttc catcaatcga tacttgaacg gatccgtcgt attcgggaaa
     5701 tccgtgcgaa ccggaaattt gctcaaggag gccgtgagct ctgcatcgag gcctaggagc
     5761 tcgccgacct tcttcgcttc ttcagccggc agtttgtggg tcccttgcag caccgccgct
     5821 acgaatgtcg ggtttctccc caccgccttt gccacctcgg caatcgtgac tttcttagcc
     5881 agccgctttg ccttgatcgc cttccgcact tcttccttct ccatcactgg cctccttctt
     5941 ggttgtttcc tgtcttattc ttacttctct tccaaaggtc aatcgctcac gaaaagttca
     6001 ccacgagcgg cgcgtctccg cctccatcgg caccggactc cccttctcca cgggcgacat
     6061 gcgaggcgtg acgcaccagg aaatgaatga cgtggttgcg aatcttgtag tagtgcggat
     6121 gctcgatgat ggattcgcgc gagcgtggcc ggggaatcgt gacatcgacg atttcgccga
     6181 cacgtgccga gggaccattc gtcatcaaca aaatccggtc agacaacaga atggcttcat
     6241 cgacgtcgtg ggttaccatg aacacggtgt tcctcgagct attccacatt tggatgagct
     6301 cctcctgaat gacacccctt gtcagcgcat cgatctgcgc gaagggctcg tccagcaaca
     6361 ggactttcgg ctcgatcgaa aaggcgcgcg ccagcccgac ccgttgcttc atccccccgg
     6421 acaaggctgt cggccgtttt gtctccgcgc ccttgagtcc gaccaacgcg atgtacttgt
     6481 tcacatgggc agccacctgg gcgtgactcc agttcggata ggccgatcgg actgctaggg
     6541 caatgttctc gaacacggtc atccacggca tcaacgagaa gttctggaac accaccatcc
     6601 gatcgagtcc cggccccacc acctctttcc cctccaaaat gacgcctccc tcggaggctg
     6661 tctccaaccc ggcgatgatg ttcagcagcg tgctcttgcc gcagcccgag tgcccgatga
     6721 ccgtgacaaa ctcgccttga tcgatcttga tcgtgacatc ttcgaagatg cacacagctc
     6781 cctcccccgt gtgactcgga aaggacttgc tgacatggtc gatttgaaga tagggcatag
     6841 gttactcctt ggatatagct ttcagccctc agcaatcagc tttcagcttc agagtaggaa
     6901 aatggttgtg cccatgctga tagctgacgg ctgatggctt ctttgctcac tcctggtacg
     6961 tgacaagtcc ggccagcttg ttgaatcccg catccaagat gacgccgaca catcccacga
     7021 cgagaatggc aaagatcacg ccggaaatat ccaggttatt ccactcgatc cagctcaaat
     7081 acccgactcc caactctccc aacagcattt cagccggcac aaccgccacc agcgcgctgc
     7141 cgaacgatat acgcagtccg ttgacgatcg tcggcgccgc ccccggaagg atgaccgtaa
     7201 acagccgctt gaaccaggac agctgtaaaa tcgctgcgac gtgcagatag tctttccgaa
     7261 tgccgttcac gccaaaggcc gttgtcgcaa gagtcggcca cagagacgcc atgaagacca
     7321 cgaggatggc cgtccatttc gggtctctga ccgtgtacag gagcagcggc atccacgcta
     7381 acggcgagat cggtttcaaa atctggatga acgggttcac cgccttaaag aggaccttat
     7441 tgagacccaa gaagacgccc agcacgaccg cgaccagtga cgcagcaaga aaccctgccc
     7501 cgaatcgttg cacggtatac atgacgagat aggcgattcc gtgatcgttg gtgcctttct
     7561 tgacaaatgc ttcgctcagt tcctcccgtg ccttttccat gaccgcgagg gggccgggaa
     7621 tccctttcac tttttccttc tctgggttcc acttgtatgt cccgtccggc aaatggacaa
     7681 tgtcgccgtt gaactccatc atctggagtt gctcttcggt caaccccttc gcatcgaacg
     7741 cctcccgcag ggtcgcgata tgccacccga gcagaaagat caccaccaga aacaccgtga
     7801 tcatccatgg actgcctgtc gcttgacggg ccatcgtgct cccctttgtc ttcctcgatg
     7861 tcactcgagg gttgcttgca tccacttcta cgggcagatt cttcgctctc aacaactccg
     7921 ccggcatgtc acgctccttt tccgttgact gcccatcacg ccatgctgtg gatggcaaac
     7981 cccttcacat aggcttcagg cttggatgga tcgaattcct tccccatgat gacgtgtttc
     8041 atcgtggtcg cctgatgagc cggataccct aattcctttt cgatacggtc gcaatccgcc
     8101 gcccgataga cctgctcggc aacggactta tagttcacgt cgcccttcaa atgcccccag
     8161 cgcttcattt gcgtcagaat ccagatcgcc atggaatgcc acggataggg atcgaaatcg
     8221 atgcgactgg gatctttccg aatgtttccc agtccatctg cataggtgcc cgtcaaaacc
     8281 tgttcgagca ccgtgaccgg ctggttcaga taattcgtcg gagcgatcgc cgccgcgatt
     8341 tccttgcgat gcgctgcatc ggaggcatag tgcgtcgcgt ccacgatggc tcgaaacagc
     8401 gcgttaaagg tgttcggata cttggtcgca aactcttccg acaccgtgaa ggcgcagcag
     8461 ggatgccgat cccaaatttc cttcgacaac ttgaagatga accccacatt ctcgtaaatc
     8521 gcccgttgat tgaacgggtc cggcgccagg tatccatcca cgttgccggc tttcaggttg
     8581 gcgaccatct cgggcggcgg caccacgcga atctggacat ccttatcggg gtgcacccct
     8641 ccttcggcta agtagtagcg aaggagatag ttgtgcatcg agtagtcgaa gggcacgcaa
     8701 aagcgaaatc ctttcatgtc ggccgccgat ttgacgtcct tgtgcttgat gtgcagcgtg
     8761 atcgcctgcc cattgatgtt ctcgacggcc ggcatgtaga agggcttggg aaccgacccg
     8821 gcacccaggg aaatcgccaa cggcatcggc gtcagcatgt gcgccgcatc gacttcgccg
     8881 ttgatcgccc aatcccggat catcgcccag ccggccgcgc gtttgactga cgcgttcagg
     8941 ccatacttct tatagaaccc caatggctcc gccatgatga tcggagtggc gcaggtgatg
     9001 gggatgaagc cgatgctcaa atccttcttc tccggttttc ctccggattc cgccgcaaag
     9061 gagatgagcc ggccaagcgg gaacacatcg gcgatcaacg ccgtcaaggt cgccgtcccc
     9121 aggagcgcga gaaaccggcg ccggcctgga tcggccgcat tgagtacggc cgctgcaacc
     9181 gcccgctcaa cactggcctt gtaatagccc tcgtctgatt gatccggcgg aatcacggtc
     9241 tctgtcctgc tcgctctgtc atctgtcatg ttgtcgctcc tttggttcat cgcaggcatg
     9301 gcattctgac ctcctactcc agcgatcccc aaaaaacaaa caggcgccct cattcccgac
     9361 cgggaacaag gacgccttcg tcctgacgtg gagtctcagc cccacttgtg cgagacgaca
     9421 ccgtctcgca tttcgcatca actaaaaagg cgcccgccac tcttttcaga atgacggacg
     9481 cctttgtccg tgtacgtatt tcctgagccc agatcgaagc gccattgctt cgattcagct
     9541 cacagggacg atttatacca agaatccccc tgaaccgtca acctatactt tcgaataggc
     9601 gccgtacgcc tacacgtcgt agtacaggaa gaattcgtac ggatgcggcc gaagtcgcat
     9661 cggatcgatt tccttgctcc gcttatagcc aatccaggct tcgatcagat cctcggagaa
     9721 caccccgccc ttgagcagga actggtagtc cttctcgagg tttctgaggg attcatcgag
     9781 gctgccgggc attgtccgga ttttggctgc ttccttggcc tcaagatcgt agaggtcctt
     9841 ttccgccggc tcgcccggat taatcttgtt ctcgattcca tccaagccgg ccatcagcat
     9901 agcggaaaac gccaggtagg ggttggcgcc cgggtcgggg aaacgcacct caatccgctt
     9961 ggctttcgga cttggcgagt acatgggaat gcggataccg gccgatcggt tccggctgga
    10021 ataggccaag agcaccgggg cttcaaatcc cggcgtcaac cgcttgtacg agttcgtcgt
    10081 cgggttcgta aacgccgcca acgccggcgc atgcttgaga atcccgccga tgtagtgcag
    10141 gcacatctgt gaaacacccg cgtattcctt tccggcaaac aacggcttcc cgtccttcca
    10201 gatgctctgg tgggtatgca tgccgctgcc gttgtccccg aaaatcggct tcggcatgaa
    10261 ggtcacagtc ttgccgtggc ggcgcgcgac gtttttgacg atgtatttgt acatcaacat
    10321 cttgtcggcg gttcgaacca gcgaatcaaa ccggatatcg atttcggcct gaccggccgt
    10381 ggccacttca tggtgatgct tttcgattgg aatgccgacc ttttccatct ccagaatcat
    10441 ctcggtacga atatcctgtt gcgtatcggt cggcggaacg gggaagtagc cttccttgtg
    10501 gcggatcttg ccgcccagat tgacgccttc ctgccccatg ttccacgtgc cttcatccga
    10561 atcgatgtag tagaagccgc tgtggctcgt ttgatcgtag cgggcatggt caaaaataaa
    10621 aaattccgct tccggccccc agtaggagat gtcaccgatc ttggtgctct ggaggtgctt
    10681 ttcggccttc tgggcgatat agcgcggatc acggtcgtag gtttcacggg agatcggatc
    10741 caccacgttg ccgatcaggc tcagcgtcgg cacggcagtg aacgggtcca gacaggccgt
    10801 gctcggatct ggcacgacca acatgtcgct gttgttgatc gccttccagc cgcgaatgga
    10861 cgaaccgtcc atgccggagc cgtccttgaa cagatcttcc gtcaattcgt tggttgggat
    10921 cgtaaaatgc tgccacgtcc ctatcagatc aacgaatttc aggtctacga cctgcacctt
    10981 gttcttcttc gcaaactcta atacctcgcg agcattcatc cccctcctcc tttcagaact
    11041 accctagtga gactacctgc gcagccgtga ctaggctgct tttgcctcga gaaaattctc
    11101 gatggccttt tcgattcccg tccgcaatcg ctccacctgc ccatgatcgt ccagcttctt
    11161 cccgtgcaga tccgtgacat agaacacgtc ggccacctgg tccagcctgg tggagatgcg
    11221 tgcggcatga atcgacaatc cgagctgaaa gatcgcgttc gtgatgacat acaacaagcc
    11281 ctgacggtcg tcggcaaaca cgtcgatgat cgtgaagcca tcggacgttt cattgtcgat
    11341 ccggacctct gtcgcttcgc gggccttcgg catggggcgc gtcagcttca accgcgctcc
    11401 gcgtctgagc aggtcgtcga catgctccca gcctttcaag accgatgcga tccgatcgcc
    11461 gacggtatgg cggcgatcgg ccggcggctc cccttggtaa tcaggatccg tcacctggaa
    11521 ggtatccacg acgatgccat cggccctcgt gagaatctgg gcgtcgagaa tctgcaaccc
    11581 gctggccgcc atcactccgg caattttcga gaagatcccc ggcgtcaact cattgtgcgt
    11641 gacgacggta tactcacaga cggacagttc cttgttgaag tccgaatcga cgagcacgtc
    11701 gcccacgtgg agccggctga ccgcggccac atgcgccgca attcgggccg gcggcgttcc
    11761 gtagaggtac cgctccggaa actgcttcag ctgcgcttcg atccaggctc gattgacccc
    11821 gctctgcccg gccagacagg gatggcggat gacctcctcc gctacttggt tcagccgttc
    11881 aggcgcaatc tgggtctctc gctcgcccga tacctccggc atcgtcctga cgtacaactc
    11941 aaccagcaac gattctttcc atttggtgag cacaccgggg ccgacggcgg caatatccgc
    12001 cgcggttagt gccagcagct tcttcaagac ttcgggcgtg ccgatttcac gggcaaacgg
    12061 caataccacc ttgacgtcgt tcggatctcg ccggaacgcc gtgtgagcca ttaggagatg
    12121 cttatggacg agaaaagaca gcgtcctcgt atcttgctcg tccaatccga ggcgaactgc
    12181 gacctcctcc gccattcgct tgccgacatc gctgtggtcc tcttcctgcc ctttccccaa
    12241 atcgtgcatg agcacggaca ggtgaaggat gtctttgcgt ttgatgctgc gatagacctc
    12301 ccccaacgca gtcggctcgt tcgccagggc ttcggcccgg ccgacggcga gcaggctgtg
    12361 ctcatcgacc gtgtacttgt gatactgatt gaattgcatc agcccgcgga cggtttgcat
    12421 cgcaggaacc attttttcga gcagaaaggc gcggtgcata gcctcgagcg tcgcagtcgt
    12481 gcccgggcct gccatgatct tcaaaaagat ccggctgacc tccggcgtgc gataggcctc
    12541 ggccgacacc gactcgacat gccggtgaat gtcttccagc aaggccggcg caatggtgag
    12601 ttgtcgggaa cgcgccagat caaacaggcg caggagcagg gccggacttt ccagcacctt
    12661 cgcgcgcgct tcttccgcca cagtcaatgc cgtgccctgc acgacaaagt attcatcgac
    12721 tcgaggcttc gggagaaatt ttgccatgcg ctgccagaac ggcacgctct tgcagcgatc
    12781 gataaatcgc atgcagcgct catgcaaccc catcgtgtgc cgatagtact gctgcatgaa
    12841 ttgctcgacc gccagcagat ggggctgatc ctgaaaaccg aaatgttggg ccagccacac
    12901 ctgttcatcg aacgagagaa tctcctgagc cgtgccggcg tgcacgtgga gcagcgcccg
    12961 cacccgccac aagaattccc gtgcctcggt cagcgccagc gagtcggctc gcgacagcac
    13021 accccgatcg gaaagttcgc gaatcgtcgg cgcccgatag cgggccatgc cggcccactg
    13081 gagcaggtgc agatcccgaa gcccgccctt gcttttcttg acgttcggct ccagcagata
    13141 caccgtctcg ccgaacttct cgtattcacg ccggcgctct tccagcttat agtcaagaaa
    13201 tttgtccgtc cctttgacga caacactccg catataccgg ctgtggaatt tctcgaacag
    13261 ttccggactg cccgccaaaa aacgcgactc catcatcgag gttctgaccg tcacatccgc
    13321 catggccagt tcgatactgt cggcgatggt ccggacgctg tgcccgacct ggaacccgca
    13381 gtcccagagc ggatgcagca cggcgcggac aaacgacttc gcagcctcct ccgctccggg
    13441 gcggaacaaa aacatgaggt cgatgtccga atggggcgcc agttccctcc gcccatatcc
    13501 gcccaatgcc atgacgcaac actgccggag accggcgttg gctaaggcct caccgccgtg
    13561 ccgcaccgcc tcgcgatagc gcccgacgat aagcccgtcg accaaatcgg tcatggcggc
    13621 gagggtctcc ccacccgacg cgccgcccat aacccgttcg ccgatgacgc gacgctgctc
    13681 cgccagaaag aggccgaggc tctcggcatc gccttcatgc gtaaagaggc cggccccctg
    13741 ttgtccggac gtgatcgtca caatgcggtg tctccggttt caccggtcct gatgcggaca
    13801 gccgcgctca agtcacgcac gaaaatcttg ccgtctccga tgctgccggt tttggctgct
    13861 cgagtgatcg tctcaatgac gcgcggcacc tgggcgtccg tcacggccac ctcgattttc
    13921 actttcggca caaattctat ggtgtattcc tgtccccgat aggtttcctt atgacccttc
    13981 tgacgaccga accctttgac ttcggacacg gtcattccct gcactcccat ttccaacaac
    14041 gcatctttga cttcatccaa tttgaacggc ttgacgatgg cttcgatcag tttcatgtgc
    14101 ttctcctcct tgcctggctt gcccgaggtc gtcgatctcg cgtcgtcgct cggatcgccg
    14161 gcatcatcga tcggtcgttt gccttatgtc catttacgaa tatgcacgct cattgtgctg
    14221 gctgaggtcc aacccggttg cctcatcctc aggtgacacc cggagaccga ccatcgcatc
    14281 aacaagccgc agaatgatcc acgtgccaac cgcagaaaag atcgctacgg cagcgaccat
    14341 gatggcctga atcccgaact gcgctgtatt gccgaagaac agcccatcgg ctcccgcagt
    14401 attgatcgcc ttggaggcga acaaccccgt cgccaggatg ccgaagatgc cgcccacacc
    14461 gtggatgccc acgacatcga gggagtcatc atagcccatc ctccccttcc aaacaacggc
    14521 gaagtagcac agaatcccag caaatccgcc aattacgatc gctgagaagg gaccaacgaa
    14581 cccggcgcca ggggtcactg tcgccaggcc ggcgacggct ccgctcgcca cccctagtac
    14641 cgtcggtttg ccgcgatgca tccactccat ccacatccat gccaaggcgg cagtcgccgc
    14701 ggccgtatga gtagccagga atgcgctccc ggctactcca ttcgctccta acgcgctgcc
    14761 tgcgttgaat ccaaaccagc cgaaccacag cagccctgtt cccaacaacg tgaacgggag
    14821 attgtggggc gccatatact ccgttccgta cccctttcgc tgtcccatga ccagcgcaca
    14881 aatcagggca ctgactccgg aactgatatg cacgaccgca ccgcctgcga aatccaacgc
    14941 gcccattttc cccaaccatc cgccacccca gatccagtgg gccaccggcg tgtaaatcac
    15001 cacggaccag agagccgcga acagcaggag cgcggaaaag cgcatccgtt ccgcaaaggc
    15061 accggtgatc agtgccggcg taatggctgc aaacatgagt tggaacaaca taaacacctg
    15121 atgaggaata gtcggtccgt aagtcggatg cggctcgctt ccgaccccgt tcaggccgat
    15181 ccactccaaa cccccgatga ttcctccctt atcgggaccg aaggccaggc tgtaaccaaa
    15241 cagaatccag atcagactga ccatgcagag aatgatcgca ctgtgcatga tcgtgcctaa
    15301 cacgttcttg cttcgaacca gtccgccata gaacagcgcc aatcccggca tcgtcatggc
    15361 caggaccaac gcggtagaca tgagcaccca ggcggtatcc cctgtatcga ctttgaggag
    15421 aggggttgcg acgctggctc ccgcttgagc catggcgtcc tgtgccattg cgactgaccc
    15481 gatgaacaga gctagagttc ccagcatgac ggccacgatg gtcaggcaca taccgccggg
    15541 cgtcccttgg cgcttgctgt tccgcttgca gtttcgtttc aggtcaatca ccatgtccct
    15601 cctcttgttt gaacacgcca tcctctaaga ataggcccgt tcgtcatgtt gactgagatc
    15661 caggcccata cgctcctctt cctcactcac ccgcagaccc attactccat cgactaactt
    15721 cagaatgatg aacgttcccg tgaacgcaaa cacgaccgag atcagcaccg ccatggcttg
    15781 aatgccaaat tgcgcaggat tgccatagaa caatccgtct gcaccggccg cattaatcgc
    15841 cttcgatgcg aaaagccccg tcgccagcgc accccagatg cctccgactc cgtgaatccc
    15901 gaccacatca agggcgtcat cgtaaccgaa cttggacttc cacagtaccg cgagatagca
    15961 gaagactccc gctccaagac cgatcacaat cgacgacatc ggaccgacaa accctgaggc
    16021 cggggtaatg gccaccaagc ctgccaccgc tccgctcgcc gcgccaagta ctgtcggttt
    16081 gccacgatac atccactccg caatcatcca tgccaacgcc gccgaggcgg tcgccgtatt
    16141 cgtcaccaca aacgcgctgg tcgccagcgc gccggaagcg actgcactgc cggcattgaa
    16201 cccgaaccac ccgaaccaga gcagcgacgc tcctaagacg gtcatgggca aattgtgtgg
    16261 agacatatgc tccctgccgt atcccaaacg ctgtctcaac acgatggcgc aggccaacgc
    16321 ggagataccg gaactgatat gaacgaccgt cccgccggca aagtccagcg ccccgaggtt
    16381 tctgacccat cccccgaccg cccacaccca atgtgccagc ggatcataga tgaaggtcgc
    16441 ccacagcaag gtgaacacga gaaagctgct gaacttcatc cgttccgcaa aagcgcctgt
    16501 gatgagggcc ggagtgatca cggcaaacat catctgaaaa atcatgaaag cctgatgcgg
    16561 aaccgtcgcc ccatagtcgg cgttgggctc caagcccacc ccgttgaggc ccagccattc
    16621 caagcctccg atgatatgtc ccttgtccgg cccgaaggcg agagagtatc cccacagcac
    16681 ccactggatg ctgatcaagc agagaataat aaagctctgc atgatcgtgc cgagggaatt
    16741 tttcgaacgc accatcccgc cgtagaacag cgctaaaccc ggcgccgtca tggcaagcac
    16801 caacgcggtc gataccagca gccaggccgt atcaccggtg tcaatcttcg gcaatgctgg
    16861 ggcggctgga gcagtcgtcg cctccgatgc tgcagccgga ggcgctgcgg gagccggcgc
    16921 gcctgcatct tgcgcataca gagccgtcgc ccccgtcaac gtgaccatgc agacaagaac
    16981 cggcagaact tttctcatag tgctgacatg tgatggcata atcgcgtcct ttccttccct
    17041 gtttagaaca ggtacatgac ttcgaacgac agggtttctt gatggttggt cgggcgggtg
    17101 ccataaagaa agacattctt atcagatttg tcgtaccgga attcgatccg cgtgatcagc
    17161 gacgggaccg gcttgaactg cagcgtgggg gtgatctccc acaacgtttg ggccaccggt
    17221 atggcggccg tggtattcgt ggccccggcg cacgtattcg tgccgcccgc gttgttccac
    17281 gtgccggtgc acacacggga tcctcccgca tcctcgaaaa tttccccgcg gaaccgcgcg
    17341 ctccattgct cgtcgaaatc gtggatcagg tagccggcta agccgttcca ccgcgcgttg
    17401 gtcttcagat tgggattcgc cgctcgcgcc gggttgttcg actcattcac gtaatacggc
    17461 tcaagcacca ccgtgtcctt ttcagtgggc ttcagcgtga agatcaagcc attggcaaaa
    17521 cgtttggcag aggggtcccc gggcgcacct cccgaggttg cgtcgccgaa ttgacggttc
    17581 gcttgctccg gtccatagga gccgtaccag ctgactccga atttttcatg cggcgtcagc
    17641 gcaaccaggt actcgatcgt cttgccccgg ttgttgtcat cgatattgtc ccagccgttg
    17701 accaccccga atgaggccgt cacgaggggg ttgaacgtat aggtgaatcg cacaccggtc
    17761 gtggtgaagg cctggcccgt tccgaacatg aaggagcggg aaaagttgac gttctcgaaa
    17821 ctgttgatca cctcatagcc gatcagcgtg ttgatcttgc cgaactggac cttgaggccg
    17881 ttgccgaccg gcaggatgta ctcggcatag acctcttgca aatcgatctc gtcgttgctg
    17941 gttcctgttt gataattggt tcgtgccctt gtcacccgag catcggcgcc gaagttcagc
    18001 cgcgcgcgaa agcccgcccg ctccactgcg cttccgcccg catcagccgg ccgttccaac
    18061 atgagttgcg ccaggtgcgg catgaaggaa ttggcgtccg tgtcgaagat gtggccttgg
    18121 ttgaggtcgc tgttggggtt gttgaaattt tgggtgtagg ccaggtccac gaatcccgag
    18181 gccttaaagc ccaaggtttt ccagagagac ggcgcatcca ctttcttctc taaggcatcc
    18241 agccgctcct gcacggtcgg cgtcgaggcc ggagccggcg cgggactttg ggcgaatacg
    18301 gagttgccgt cgatcagcaa gaacagcgca cacaacccta ttccgatgag actgcgtcgt
    18361 gacatggtga cgtcctccct cgtctgatct ggtgattgcg ttcttcatac gccttgcctc
    18421 actcaccacg acgacgtcgt cgccacgcac gcctttgtgc gcaacatctc gtttcttccc
    18481 gtccacctga cttgttgtgc cacaccgatt cccgccagcc gacgcgagaa catcgcgggt
    18541 cagcggagat gcgcctgcgg gcggcgtctg cccagtgttt cccgcgactc gattcatcag
    18601 cacgatcccg attcagtccg cacgtctgtg gtcaaaaaaa aacgtcctct agccccttgg
    18661 gccggaggac gccattgtcc acgccgtgat gttatgcagg cggacgggaa acgcctttgt
    18721 ttccctgcca attttcggga cttgtagcat ctagacagag aatctgtcaa cttgttttcc
    18781 gctcgaccag catgcccaat ccctcttttt tacctcgaac ggtggcattg acagcgttgc
    18841 ctttgcgaga caatttcatg cagactgtag gcttgattca ataccctaat caaaacaaga
    18901 ggttctacag gatattctct gtggaaaagg agaagatgga gcgaatcacg tttgtgatgc
    18961 tggttggaat ggccggcaca atctttctcg tcgatcttct ttccccgccc gggttggcat
    19021 ggtgggcgct ctatctcttt cctctcatgc tggcattctc tcttccgaac cggatggccc
    19081 cagtcctctt tgcggtgatc gcaacggctc tgatctcaat cagggcattc tcctccccct
    19141 cgacgtctcc aacatggttg acgttgggtc ccagcgtctt ggcactggct gtcttgtggg
    19201 ggagtgtggc ccttctcgga catcttcaga agaagtggcg tacgatatcg gcccatcggg
    19261 atgtctcgca gcacgaagaa cagagacgac ttcgtcatct gttcggcgaa cgagtcaaag
    19321 agttgaccgc gctccaccgg accgtgcgtc tcttacaaga tagtgccaaa ccgatcgaag
    19381 aggcgctgag tgagatcgtc gcgttgctcc ctcccgcctg gcaatacccg gagattactg
    19441 tcgcccgcat tacctttgac ggcatggttt tcaccacgcc cttcttcgtc gaatcgccgt
    19501 ggaaacagac cgcaacctgg gtcacaccgg acggcgccaa ggggatgatc gacgtgatgt
    19561 atcgggagga gcggccatcg gaggccgaag gcccgtttct cgctgaagaa cgcgacttga
    19621 ttcattcgct tgccgatagc ctctgctcct atctcactcg ccggcgtgcg gaactagccc
    19681 ttcgcgaagc ccatgagcgc ctgaaagctc tctcccatca actgatggag gttcaggaaa
    19741 acgaacgtcg tcgcatcgcg catgatctgc acgatgagat cggccaagcg ctgacggttg
    19801 tgaaaatgaa tctccaaacc atgcaacgca ttgccgacac atcggcgatt gctatccctt
    19861 tgatggacag ttcagctgtc atcgatcaga cactccaaca tgtccgcgat ctctcactcg
    19921 acttgcgccc ttccctgctc gacgacctcg gacttgcacc ggctgtccgc tggtatttaa
    19981 gtcggcaggc agaacggact ggctggacca tcgacgtgac ggtggacgag tctcttcctc
    20041 caccgccaca acccgtcgcg atcgcctgct tccgcgtact tcaagaagcg gttacgaaca
    20101 tcatgcgcca ttcgaacgcc tccagcgtga gcgtgtcttt acggcagcat gaaggcgatt
    20161 tgctcttagt cgttcgtgat aacggcgtag ggttcgacgt ccggaacgcg ctcgacaaag
    20221 cagccagcgg gcagagtatg gggttaatcg gaatgcagga acgcattcgg ggcatgaacg
    20281 gttccatatc catcgagtca gagccggggc agggaaccga ggtccgggtt cgtattcctg
    20341 tatcccgcct ctcacaacct attcgggccg aggtttcgac ctaatcatat gggtacgatt
    20401 cgcgttctgc tcgctgatga tcaccgcctt gtacgggcgg ggcttcgcgc cctcttgcaa
    20461 agtcttgcag gggtccaggt cgtcgcggaa gcaggaaacg gctacgaggc catccaactc
    20521 acggagcaac accagcctga tgtcgtgatc atggatatcg gcatggaggg actcaacggc
    20581 ttagaggcgg cggccagact ggcgaaattc actccggcgc ctcgggtcat catcctctca
    20641 atgcatgcca atgaagaata tgtccggcgg tccctgcagg cgggagcggt gggttatctt
    20701 ctgaagggtg cggagccggc cgagctggaa ttcgcgcttc aggccgtggt gcgcggagaa
    20761 acctacctca ctcccgccgt gtcaaagcag gtggtgcaag actatctcca gggtggcggc
    20821 gtgaaggcag atcctctcca ggagctcaca cctcgccagc gcgaggtcct ccaactcatt
    20881 gcggaaggcc actcaaccaa agagatcgca tccaaactga acctcagcat caagaccgtg
    20941 gagactcacc gtagcgagtt gatgaaccgg ttgaacattc acgatgtggc aggtttagtg
    21001 cggtatgcca tacgaacggg cctcgtaact cctgattcgt aatcctcccg gacccccaag
    21061 attcctttca acttttcaat gtcttaggga aacgttcccc cctgatctca ggggatttcc
    21121 cccctcattc tcatgttttc cccgataccg caggacgcga tctgatcgga taatgacaca
    21181 gaggaagaaa gaacagatgc caaacaaata cagaatcatt caaccatcat tgagtgaggg
    21241 aggtgcacga tgacgaggat tagtcaggat ttgacaagga agccactgtg gatgctctca
    21301 gcgatgttct tgctcctgcc gatgggaaat gtcggctgct cgttcgcaac ggatcaggtc
    21361 ctcccggcac aactcgaaag tcgcgtctca tcaaatgcga ctgccgatga tcatctcgcc
    21421 gcggccctgc tgtatcaaga acaggcccag ctagccaaaa ccgaagcggg caagtatgag
    21481 caggccgctg cttcgatcag ggcgattgaa gatcccaaag ggttccgacg gggtgcactc
    21541 atgacggcgg cccaggagca ccggcggacg gcagacgaga tgcaacagct ctatgcgtcg
    21601 catgaggcga aggcccagac catgatgggc aagcagcagg cccaataaga gatttttcgg
    21661 ggaggccggt tatccgcact ggacggtggg ctgatctcga ctgtttggcc gatcagccca
    21721 tcggactggt aaatctgctc tctgccgcac gttcttgcgt tccgctacca atcctatcgc
    21781 gagcagtgct gttcgctttc cttacttctc tttctctgca tgcatcgctt tctccagtaa
    21841 cagctccgcc tcgagccgat ggacattcgc acgaaagcga aattcggtcg ccatggtgct
    21901 gtgcgagtct gccgccattt tcatcccctc gcgccggaac cctttcgtgt ccataagtgg
    21961 tgtaatggca gccgcttttt gttcataatg cttcgcttgg gcttcatggt tctccgcatc
    22021 cttgtcatac tgtttcgcga catcgagatg ttcctgcgcc gcttccttca tcacgtctcc
    22081 acccgcgagc gcttcaggga gcccgcccgg gccagataca tcaggctgat gccacagtgc
    22141 cggcaggccg atggcgacaa gcgcgagagc ccctatgaga cctacgatca ttcgcgtccg
    22201 taccttcatc gatttatacc tccttgttta ttgagaaaga attgcggtcc ggctcacccc
    22261 ggatcatccg atcggacagg gcaatcttct gacatcagag cgattgaccc acccagccag
    22321 tccctctgtc gtcatcacac gaaaataggc cgcatcgacc gattcgtcca cggccatcag
    22381 agagccctgc tcgaggactt ggatgacttt ccctcttcca tgctgtgtcc tgacggcagc
    22441 cgcctggtac cgtggagcaa gacagaccaa aggggaagtt gctacgacgt cctggtccga
    22501 ccggcgtgcg acagaggttc ctccagacgt ctcggattgt gaaacagccg atggaacccc
    22561 ctttccggtt gaaggagaag gggctatgga cgccttctcg tcggtctgcc aatcagatgc
    22621 aaatgattct gtcgcaatgg agttcgtcac ttctcttgcc actttcatgt gcgcaacaaa
    22681 tgcttggcca atatgaggca ggagactcgg aagggacttc agcctgtttt ctgccaggcc
    22741 agtaaggccg tcgctgcttt tcgagaccaa gcgactgtca atgaggtgga ctcggtacgc
    22801 cccggcatag agctgaatgc aggcggtata ccgacggaga ccttgagatt cagagtgatc
    22861 cgccacccac gcgtgcacga tcgccagact gcctggacat gcgacctcac gaattgtgac
    22921 catgcccaga cgttcgagat gcactcgccg atcttccacg gtgaaagggg taggcctcgt
    22981 cggcaggggg gagagaacac tcccctcgat gatccgaggc gtcgtcgcaa gggtttgaac
    23041 aaaggccagc tc
//
LOCUS       DATIFR010000086        32408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2350769, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000086 DATIFR010000000
VERSION     DATIFR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32408
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350769"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VLD60_04815"
     CDS             <1..379
                     /locus_tag="VLD60_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44311.1"
                     /translation="RREIHRRQQLYRQGRHPLVLKDRIAIVVDDGIATGATFFASVEA
                     IRQQAPGQLVAAIPVGPRETIAKARSLVDELVVLATPEPFWAVGNHYVDFTQVEDRDV
                     LEYLNLADESLREWRERIHSPSR"
     gene            402..902
                     /locus_tag="VLD60_04820"
     CDS             402..902
                     /locus_tag="VLD60_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HSB44312.1"
                     /translation="MAAKAKATARRGARRDRLVQEYQHDTYKVRGKLKEPTVCSGCGA
                     LFHKGRWTWGSVPRGAHSTTCPACHRTHDKYPKGLVTITGNFKEAQHEQVRGVIRNTE
                     AREMKEHPLSRIMAIERRPEGLVISTTDTHLPRRIGEALTHAYRGDLNFHYDRDEDFI
                     RVNWIR"
     gene            951..1418
                     /locus_tag="VLD60_04825"
     CDS             951..1418
                     /locus_tag="VLD60_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB44313.1"
                     /translation="MKVGDIMTREVQCCGPDTNLAAAAKMMWDSDCGVVPIVNAQGRV
                     MGMLTDRDICMAAATKTRPPASITAWETSSGKAITCRPDDDIRAALELMERGKVRRLP
                     VVDEDGMLQGMLSMNDLVLAAGEHRGRSAPELSVDDVMRALKAICGHRIHVEL"
     gene            1431..2288
                     /locus_tag="VLD60_04830"
     CDS             1431..2288
                     /locus_tag="VLD60_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB44314.1"
                     /translation="MKIVVGVDWSEQAFATVQQVLHLYRPSELTLVHGVDLGIFEYPI
                     VAQAANLQGYDDFRNSLIDAGRQVLARTANMLPFDSDTVKQVNEVGNPAEIILKTANA
                     VEADLVAVGARGHSRVTELVLGSVSHRVLMHSTRPTLIVKGTSRPIQRVLVAIEGGED
                     AERIKQWLLVHPFANPVDLCVLSVVVPIRVADPYNIGGFETWSDSLKTYAEDLVKTVG
                     AELMGSRYSISTRVATGEVAATVAEQAKGMDLVVVASHGRKGLERFLLGSASHGIVHH
                     VTCPILVIR"
     gene            2301..2660
                     /locus_tag="VLD60_04835"
     CDS             2301..2660
                     /locus_tag="VLD60_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44315.1"
                     /translation="MKTILTVTVLLVFAATSWVSAQGARGNPKNGQIVFEQHCLRCHG
                     SKLDGNGPDAKDLIVRPANLQSQGSRAKTDWELLVTITNGALFTPMHGFRGKLTDEQI
                     LDVLSYIRSVVPFDAVS"
     gene            2787..3821
                     /locus_tag="VLD60_04840"
     CDS             2787..3821
                     /locus_tag="VLD60_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdH-redoxin"
                     /protein_id="HSB44316.1"
                     /translation="MFVRSGCPHCEAAEHFIDQLRRERPSLHIVFHDVAQDPTALQRL
                     KTLAAEHGFANVGVPTFLIGEEVLVGFLSAETTGAEIRARLDKRVQSDRASPVTESIK
                     TTWFGELRVNDMGLPLFTLAIGLLDGFNPCAMWVLLFLLSLLVNLHDRLKMALIAGTF
                     VAVSGLLYFAFMAAWLNVFLLVGLSRAVQIGLGGIAIFVGTVHVKDFFGWQQGISLSI
                     PESAKPGFYARARRILQAENLAGAMAGIVVLAGLVNMIELLCTAGFPALYTQILTLQQ
                     LPIWQYYGYLGLYNLAYIFDDSLMVTIAVITLGHRKLQERAGRWLKLTSGLVMAGLGS
                     VLLLEPKWLI"
     gene            3871..6039
                     /locus_tag="VLD60_04845"
     CDS             3871..6039
                     /locus_tag="VLD60_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSB44317.1"
                     /translation="MAGASQKYGVESLKPFFYPRRVAVVGASRERTGVGHRILETLQG
                     SGFTGSIIPVNPHANEIAGLHAYPSLSAIPDPVDLAVIAVPPHLVLSIIDDCAAKQVR
                     AVVLITAGFAETGGSGTSLEQQLHVKVRQYGIRLIGPNCFGLMNLDPAVRLNATYTPA
                     FPTTGRVAFASESGGLGLAVVTAARRIHLGLSSFVSVGNHADVSINDLLEYWEQDDAT
                     GVILLYLETIVEPRRFRLLTERVGKKKPIVVLKAGRTLAGQSAAGSHTAALATDETAV
                     NALFKQCGVIQATSLEDFLALATGLSNQRLPAGRRVGILTNSGGPGVLCADSCAEEGL
                     SLPELSERTRSTLASFLPPTAALRNPVDVIGFATEEQHALAVETMLKSDEIDTLIILH
                     VSVRANDNDPVAAGIIRGISTARKAIGRNKPVYLCWMAEGDLERTFMVGGETIPTYPH
                     PEIPARIISRALTYDDRRHQPAGQSPRYSDVEFAKIKAICAKALSERGSGWLTTEETF
                     AVLALMTLPIVQGAVAVSAEEAGKLAHKMGFPVAVKLASRHIVHKTGIAAVRLNLQSE
                     QEVRDAFHAIRMRLLQDRKLDAMEGVLVQPMVSGGVEVMAGMTRDPLFGPLLAFGLGG
                     IHVEILRDMQFRTAPLTEHDARAMVEEIKGHRLLTGYRGQPAVDAKAIEDVLLRISRL
                     VEEIPEISELDLNPIVVLPDAQGCKIVDARIRIVKDTQKV"
     gene            complement(6493..6879)
                     /locus_tag="VLD60_04850"
     CDS             complement(6493..6879)
                     /locus_tag="VLD60_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44318.1"
                     /translation="MPMQTFLICCAVSVLFLGASINGDGHFALTSEGGSQGTDKDSPS
                     GMASRRPAGDHTRGEKLYNASCVVCHGQRASGGIGPRLTGNPVLSNEQAFWKTVYEGR
                     HVMPPLKDAVTEQQMADIRAWLQTLR"
     gene            complement(7027..7878)
                     /locus_tag="VLD60_04855"
     CDS             complement(7027..7878)
                     /locus_tag="VLD60_04855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HSB44319.1"
                     /translation="MSKRHEIDDHIRTLGEISEIMSAMNNLAMMEIHKLTRLLSAQQR
                     VVVSMETAARDFLAFHPEALPRDRGGTPLYLVIGSERGFCGDYNGRLLSELERHLHTA
                     GEKEPLMVVVGRKLSGRMAGDPRLVAAIDGPTVSEEIPRVLVEIIQRIHDLRQERQDS
                     RTFVMTAVYRVAGTDGITIQPLRPFETIPRVETGGSTAPLLTLPPRVFIAELVDLYLF
                     AILHEIFYSALMAESRERLTHLDGALKRLEKETSELRLRRNILRQEEITEEIELIMLS
                     AEQLKSQ"
     gene            complement(7875..9362)
                     /locus_tag="VLD60_04860"
     CDS             complement(7875..9362)
                     /locus_tag="VLD60_04860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSB44320.1"
                     /translation="MPNQSLSPNQLLAGQAAWLRDYRFGLRVDEQGTVVAVGDGIAWI
                     EGLPSAAMDEILDLEDGSRAMVFYLDTHVVGAILLEQTSRLTAGTIAHLSGRRMGVPT
                     GDALIGRVIDPLGNPLDGLDRPDCSASGLLDILSPPITARDFVHEPLYTGSKIVDTLI
                     PIGKGQRQLIIGDNGLGKSSLAIDAVINQRGRGVRCVYVMVGQKRSSVVDTIEILRQY
                     GALDHTALVVAEATSLPGLKYLAPFAGCAVAEHWMWQGSDTLVIYDDLTTHAEAYREL
                     SLLLRRPPGREAYPGDIFFLHSRLLERSTCLAPAHGGGSMTALPIVETKEGEIAAYIP
                     TNLISITDGQIYFDKRLFASGILPAIDVTKSVSRVGGKGQHHRIKEKAGRMKLDYLQF
                     LELEVFTRFGSRLEASMEAKIARGRVLRELLKQDRLSPLAIEFHLAWLTGFNQGLFDK
                     VPPDSIPALLEDLAKHVRESPLTLEAGEEEWTEAVGHWLRKDRSP"
     gene            complement(9355..10101)
                     /locus_tag="VLD60_04865"
     CDS             complement(9355..10101)
                     /locus_tag="VLD60_04865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HSB44321.1"
                     /translation="MELDWTTFILELVNFVVLIWILNRFLYRPVMDVIVQRKAAIQKT
                     LAEAETTRSNAQALQSQYENRLTEWEQEREKARTQLHDEIRVERNRLLEDLRAELDQE
                     RQKAAAVEQRRLKDFTQRAEASAIALGGTFVSRLLSRLGGPELERKIIDMVTEDLSHL
                     PDDQMQVIRATSAKTGQPMKLTSAYPLNTAQREMLSQACRTVAGRDIICEFFEDRTLI
                     AGLRISFGSWMLRANVQDEMNFFVESSRHA"
     gene            complement(10160..10429)
                     /gene="atpE"
                     /locus_tag="VLD60_04870"
     CDS             complement(10160..10429)
                     /gene="atpE"
                     /locus_tag="VLD60_04870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSB44322.1"
                     /translation="MRDISWFALASTVVAALAIAIGTMIPAMAMGRAISQALEALARQ
                     PEAERSITRALFIGLAMIESLAIYCLVIVLIILFRNPLLEYMLKP"
     gene            complement(10432..11121)
                     /locus_tag="VLD60_04875"
     CDS             complement(10432..11121)
                     /locus_tag="VLD60_04875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSB44323.1"
                     /translation="MGSSDLFRAKGLQLGPLFLTEPVLTTWGIMVALIAGSWIVTMRL
                     SLDPGPVQVVLEGLLNAADEAIRAVLPAQTARLLPFIATLWLFIIVANLSGLIPGVHA
                     PTGDLSTTAALAVLVFLSVHWFGIRAEGFKAYARHYLAPMPLMLPFHLMSELTRTLAL
                     AIRLFGNIMSLEMAALLVLLVAGFLVPIPLLMLHIVEALIQAYIFGMLALIYIAGAIH
                     SQQSQPPQAQE"
     gene            complement(11125..11394)
                     /locus_tag="VLD60_04880"
     CDS             complement(11125..11394)
                     /locus_tag="VLD60_04880"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSB44324.1"
                     /translation="MKSENQLKEEVAKQIRRMKQAEHDRPTLLRQTVYLGTLGLLFVV
                     PVIVGAYVGRWLDGLSAGYSMRWTLSLIILGVGVGAMSVYFNVKE"
     gene            complement(11391..11801)
                     /locus_tag="VLD60_04885"
     CDS             complement(11391..11801)
                     /locus_tag="VLD60_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HSB44325.1"
                     /translation="MKSFVLHLQDATHYERIDQVTSFVGQDDSGLFGILADHARMMTV
                     LAFGLARYRTTDDRWHFLAVPRGLLYCVDNNLYLSARRYIRDDEYVRISQALEEQLVT
                     EETALRTIKDSLHRLEEEMFKRLWRMGRGEYAAS"
     gene            complement(11798..13213)
                     /gene="atpD"
                     /locus_tag="VLD60_04890"
     CDS             complement(11798..13213)
                     /gene="atpD"
                     /locus_tag="VLD60_04890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSB44326.1"
                     /translation="MQNVGSAVNSNGPHVDDSLIGTIAKVHGPVVDISCTRLPPLHQA
                     LRSALNHETYIFEVYQHLDEHNVRAITLHRTGGLRRGMPVFDTGAPVHVPVSPDSLGR
                     VLNLFGKPLDGGAPLATREFRNILAQPAPLHETKPASGILETGIKVMDLLFPFIRGGK
                     TGLFGGAGIGKTVLLTEFMHAIVALHRGVSVFAGVGERIREGHELWHEMQKAGVMPQT
                     VMIFGQMDESPGVRFRVGLAAVTYAEYFRDTLQKDVLFLMDNVFRFVQAGSEISGLLG
                     RMPATVGYQPTLMTEVAELQERIISTTKGAITSVQAVYVPADDMTDPAVSGILNHLDT
                     LVILTRAQASKGIYPAVDPLQSTSKLMDRHVLGDRHYRIAEGVREHLARYRELEDILT
                     MLGIEELSETDRRIVVRARKLQRYLTQPFHVTAELTGIRGASVSLADTLLDCEAFLRG
                     EYDDVPEDRCYMRASMKGVRA"
     gene            complement(13459..15978)
                     /gene="glgP"
                     /locus_tag="VLD60_04895"
     CDS             complement(13459..15978)
                     /gene="glgP"
                     /locus_tag="VLD60_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSB44327.1"
                     /translation="MLAYFFNRPLPPGLEGLADLALDLRWTWSHVSDRLWETLDPDVW
                     ERTENPYFILQNVSQSRLEEVAKDPSFKEEIQKWLAERQRYLQDAGWYGRERAASGLK
                     TIAYFSMEFGLGEAMPIYSGGLGILAGDYLKTASDLGVPVVGVGLLYQQGYFRQILDP
                     NGRQQEVFPYNDPTSLPVTPVQNREGGWLRIRLELPGRPILVRVWQAQVGKAKLYLLD
                     SNDLLNSPADRGATALLYPSGVRPRLTQEILLGVGGWRVLEELGLDVEICHLNEGHAA
                     FAVLARALSFMRRTGQSFSVALWATRAGNVFTTHTPVEAAFDRYPLELIRPYATYLAE
                     LTHVPIQDLLALGRRDLNNGDEPFNMAFLAMRGCGVVNGVSKLHGTVSRHIFQPLYSN
                     WPQYEVPIGHVTNGVHVPSWDSQEADTLWTCTCGKDRWLGRLDELCSLIEQLKDSDLW
                     SFRASQRQALIRYARHRLVRQMQMRGAAPDHVRQAGHVLDPNAITIGFARRFAEYKRP
                     NLLLHDSDRLARMLCNPERPVQLIVAGKAHPNDEEGKRLVHELTGFAVRADVWDRVVF
                     LEDYDIALAQYLVAGIDVWLNTPRRPWEACGTSGMKVLVNGGLNLSELDGWWAEAYNP
                     EVGWALGDGREHHEPEYDAVEATRLYEILEQQVIPEFYDRDRAGIPTTWLKRIRASMA
                     RLTPQFSSNRMVREYVETIYLPAYAALKRRIANGAKLAIELEEWHARLREDWGGVRFG
                     DMQVTKTEKCWHFEAQVYSGDLEPHRVQVELYADPPNETESPTRIAMSRQEAIPGTVN
                     GYVYKADCPLTRPTHDFTPRIVPFHPDALIPLEEPLIVWKR"
     gene            complement(16252..16725)
                     /locus_tag="VLD60_04900"
     CDS             complement(16252..16725)
                     /locus_tag="VLD60_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44328.1"
                     /translation="MTSFGGVAEKGFAWAGISMVICLAVGWWTPLIDVFGGGRSTRTT
                     ARAEGNAEAGRIVFNGKGVCYYCHGIDGRRDQLPQLEDGTAELIAQLHPQPTDLRNPK
                     TLHLKTNKQRARIIREGHTGTGMFPDTRMTDQELADTLAYLATIRREGLFKSPSQ"
     gene            complement(16788..17339)
                     /locus_tag="VLD60_04905"
     CDS             complement(16788..17339)
                     /locus_tag="VLD60_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44329.1"
                     /translation="MTDITLNLDGAFPSEQALREMIATRKVCYDHDPFYVRTGTGEVV
                     KIGFQLNLYAAFQDPRHLPIGEDAEYRELIGDLRRLCRVFFQSLELLKPCEYPDPPGH
                     RIVYSPERGYRAEVCLQIPIFDREHFGAKVGRGIEELLTTAECLLRHLGSRRGRWEVP
                     PPSVEPDACLAHAEATRQALEQH"
     gene            complement(17336..17821)
                     /locus_tag="VLD60_04910"
     CDS             complement(17336..17821)
                     /locus_tag="VLD60_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSB44330.1"
                     /translation="MSEPETISAFFEQDHDRLDALFQSFQVLKRHNFTKATEAFKEFK
                     FGLQRHIVWEEELLFPLWEEKTGMSEGGPTFVMRNEHRQIGEQIEAIHRKVAEQNPDS
                     DHEEQALLNILTSHNLKEERVLYPSIDQVTTEDERKTLFQNMKEIPEERYSMCCGENH
                     T"
     gene            complement(17845..18300)
                     /locus_tag="VLD60_04915"
     CDS             complement(17845..18300)
                     /locus_tag="VLD60_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44331.1"
                     /translation="MGTLCGVLFTGCTDVDHRHTEERPNPYLTSDTRQAIPLSSSARK
                     EHRAVMLQHLETIQVIVNALVEEDYELAKGLTESHLGFFMHRQAMAQQQPENFPRAYH
                     DLAMAHHQAAEELAQIIPTKDLKQIMPRFNNVLKACVACHLEYKVREQS"
     gene            complement(18402..19349)
                     /locus_tag="VLD60_04920"
     CDS             complement(18402..19349)
                     /locus_tag="VLD60_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSB44332.1"
                     /translation="MGWEPHIFKELQQIPREEHVFPGTPLCAGCGGLESLRLASKILG
                     SHVVYVNAAGCFTNLATFPFTPLDASWLYTTMGSAPAGAQGVRDALDVLIAKGRLPKE
                     EDVKVVVLGGDGSTYDMALSSTSGAITRKLDFYYFCYDNEAYGNTGVQLSPATPYGAR
                     TTTSPCGVQHPAGTTQEKKDIFEIWRAHKPPYMATVSPRYPLDLEEKFVRAAKYPGPK
                     LFLAFAACPTGWLFDPANTPDVARQAVECGLWPLKEAINGEVVHTYMPKLQPVDDYLK
                     LQGRFRHLFEPTVQTEAISHIQGRVNAYWEQVRKAEKTA"
     gene            complement(19358..20593)
                     /locus_tag="VLD60_04925"
     CDS             complement(19358..20593)
                     /locus_tag="VLD60_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase"
                     /protein_id="HSB44333.1"
                     /translation="MTAEMMTGNLAASWGARLADVDYIPSFPITPQTEIVETLSKWCG
                     NGALNARFLTLDSEHSMMTAAGAAAATGARVFTATSSQGLLHAFEVLYSVAGWRVPLV
                     LVNVSRALASPITLEPDHNDVLAARDSGFVQIHTETCQEIVDSILMAYRIAEDPRVCL
                     PVMVNLDGFVLSFTREPVILPDANKVRAFLPPFRPAHLGFKASAPHALGTAVLGGTTY
                     SYFRHQMHLASMNALQVHEEAAADFERLFGRRYDVIEGYRLDDAEDVLVMTNAYASKG
                     KVAVDVARVEGRRVGLLRLRLIRPWPADAIRHALQGRRAVAVLDQNLAPGQGGILFQD
                     IAACLYHEALRPRALCSFIGGLGGKNISEGEFRAIFEQTAEAGVRGKGIGPVLLYTEA
                     EHREMEQLQYLAHELKTGG"
     gene            complement(20590..21495)
                     /locus_tag="VLD60_04930"
     CDS             complement(20590..21495)
                     /locus_tag="VLD60_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSB44334.1"
                     /translation="MTAIRFHGRGGQGAKTASRIVGTAAFLEGYQAQDSPVYGAERRG
                     APVSAFTRIAREPIRERGLIARPDLVVVADASLISDPPARVTEGVDERTVVFVNSSLS
                     ADDVRSQTSLPGRLTILDLNEMALQHFGKRGAISALLGAVAGRLVGLRRESIREAIAR
                     ELTDLGLVTTVIERNQTVALQCYDRVQTVALETSARQPAATAQLRPPTYESPTRGTAS
                     ITAGPNSAIRSMSGWRTFRPVLHPDLCNGCWLCFASCPEGAITIKPDGKPAIYYPHCK
                     GCLDCVEVCPTDAITAEREAQGAQS"
     gene            complement(21505..21816)
                     /locus_tag="VLD60_04935"
     CDS             complement(21505..21816)
                     /locus_tag="VLD60_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSB44335.1"
                     /translation="MENQNGKCSGQTVALAFLGGAIAGAIAGILLAPKSGEETRRVLK
                     GYARKAEEEVIEKAKEARAALDEVIERGKHFMAEKRADVEAAVKSGKEAIKDKMEKCC
                     S"
     gene            complement(22049..22579)
                     /locus_tag="VLD60_04940"
     CDS             complement(22049..22579)
                     /locus_tag="VLD60_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB44336.1"
                     /translation="MMKVWKSVFGILVVSLGVPAVAAEQHMMQPRVPADKLAEARALT
                     SPFPESPEMLAKGKALYEGKGTCMNCHGQSGAGDGPVAAPLNPSPRNFHHHGFWRHRT
                     EGEIFWVIKHGSAGTSMIGFGDQLTDEEIWAIIQYERSFAGEHGPGAMGQGRGRGSMR
                     ESGGMGPGGEGCCSDR"
     gene            complement(22652..23686)
                     /locus_tag="VLD60_04945"
     CDS             complement(22652..23686)
                     /locus_tag="VLD60_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HSB44337.1"
                     /translation="MEANNHARNPLRQLVYAASVLALVIAFGTAGYVLIEGWSAFDAL
                     YMTVTTVTTVGYREIYPLSKAAQAFTLILILAGVGTLFYVLGNFARFVLEGELQHVFG
                     RYRSGGKVKTFSHHYIVCGYGRMGQHICKELRAKPYAVVIVEKNPDRVAAAQRDGFVA
                     VEGDATQDHVLVQAGVDRAKGLVSVVNTDVENLYIVLTARGLNKDLYIVARAGEEGSE
                     QKLMRAGANRVSSPYLIGGMQVAQILLRPNVVDFLELATKSEHLDLQIEEITIEKGSR
                     FDGKTPCECGLDQDRGLIMVGIKRSNGHLEFNPGPQVMLEEGDTLIVLGQPQSLKKIQ
                     TALRSPLGAG"
     gene            complement(23746..24159)
                     /locus_tag="VLD60_04950"
     CDS             complement(23746..24159)
                     /locus_tag="VLD60_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44338.1"
                     /translation="MKRTYGNCRSNWFGPNDGCEQTGSALRFWSKEEPQHRFDVRRQN
                     PLKQWEFSPVDEKAQELWDSYTRYKEEMFSKTHTTFCPWITVKANDKQAARLESLRYV
                     LNLLPYKGKEEAQVRLASDPNVVRRFHHQMAELDF"
     gene            complement(24338..24697)
                     /locus_tag="VLD60_04955"
     CDS             complement(24338..24697)
                     /locus_tag="VLD60_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSB44339.1"
                     /translation="MATLIISGSRGTDDPTMATLPFMAAKVAKEQGHDVVLWLWNEAV
                     TLGRKGTADHVTGVNLTPLKDLLAAVQAAGVQIWVCGACAVARQISSGDLVAGASIKG
                     MPDYIKAVAERDRTVAF"
     gene            complement(24788..26191)
                     /locus_tag="VLD60_04960"
     CDS             complement(24788..26191)
                     /locus_tag="VLD60_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB44340.1"
                     /translation="MNVRRGVMLGGALLTLVGLMSFPRLLFGSDQSRAKELIQQACVQ
                     CHRLEGQADSRFNLRAPDLIWAGSKYQRPWLIRWLTGKEAPLYAKGYRWDLSEGSVKH
                     PVVSEADANAMADYFEKNNKDPRVKIGAFDVSKVSKFEVTFGGMAYKAHACLGCHTIE
                     ENGKLIGGPQSAALQNAGQRYNMDWLYRFGQNPQDFIIHTGEFLADATDPQLRAVIGY
                     LAAQGVPDFKYYEPWTTQEFGMASVDRGKVLYKEYCAQCHGFTGKGDGPAASGLEPKP
                     AIHANIPFEKLPIDYLYNVINHGGAAMGKSPSMPYWNLTIGQQGVADVIAYLKATFKG
                     APEAAAPSGGQGDACVQPRKTAKAPDDFLARTNPFPASVGTVQAGKTLFLKTAQPVAC
                     AMCHGEQGDGRGFMGAALIPPPRNFTCGGMMKNIPDGQLFWIIKNGSPGTGMMSFAGL
                     PDEQVWQLIHYIRSLAK"
     gene            complement(26231..26878)
                     /locus_tag="VLD60_04965"
     CDS             complement(26231..26878)
                     /locus_tag="VLD60_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB44341.1"
                     /translation="MSVRPETSGVRREKGTVIGLLLLGGLLFIAMTTAAQEDDLATVS
                     AALAPRAEGLIIARCSVCHSPDLITQQRLPRVRWEATVEKMKHWGAEISNDEADLLVR
                     YLSARYHPGAPDQLAPLDIDLRKAEPLAPEPAAAGPLVGVATRGAGIFEHNCQACHGT
                     GAMGGMGPKLAKNPILKHEDLFWETVLHGRGPMPAWGSVLSHQDIADIHAWLSTR"
     gene            complement(26875..28056)
                     /locus_tag="VLD60_04970"
     CDS             complement(26875..28056)
                     /locus_tag="VLD60_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HSB44342.1"
                     /translation="MNVSRRAWLKNLLYGLGAGLAVSGCRSPVQAGEEDAAARDAGPL
                     KVHVSRPFDAETPVHEFTSFLTPNHRFFVRSHFGPPVPDLVSEAVWKLRVGGLVERPR
                     EFALKELRQMEAVTVTAVVQCSGNGRAFHRPKVPGVQWERGAVGNARWTGVRLRDVLS
                     QAGVKEASRHVQLQGADRPVVASVPLFVRSIPLEKARHPDTILAYEMNGRPLPLLHGA
                     PVRVITPGWMADSCIKWLTDITLQAEEAQGYYMQTAYRVPVTPVTPGGSPSGQAMIPV
                     EAMVVKSLIAAPQEGAIVGAGPISIQGVAWGGEVKVVKVEVSVDDGKNWEPTRLVGED
                     QPYAWRQWQYVWKAKTAGMFTILCRATDERGNVQPAASPWNPSGFLWNGWDRVTITVA
                     A"
     gene            complement(28080..28490)
                     /locus_tag="VLD60_04975"
     CDS             complement(28080..28490)
                     /locus_tag="VLD60_04975"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB44343.1"
                     /translation="MKLQVAFHGGTRYDITSGKHRVVTDQPFEEGGQDAGMSPVELFV
                     GSLASCVGYFVGRFCARHDIPRDGLTVEAEWAMAEGPHRVGQINLVIHLPHRMTPEQK
                     EKLLKVAHGCTVHQSIAAPATVAIKLNPHSHAVS"
     gene            complement(28487..29017)
                     /locus_tag="VLD60_04980"
     CDS             complement(28487..29017)
                     /locus_tag="VLD60_04980"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HSB44344.1"
                     /translation="MTLALGLVLGALFGAVLQLGGASSHQKMTDALRLRDLTMIKLIV
                     MAIGVGLIGVNLLDAIGVANMKVKDLFLPGVVIAGAIFGVGFAVAGYCPGTALAAAAE
                     GKPDAWATILGGLLGALMFAFMFPELEESLLRIGRYGPLTIPGTLGLPGIIIAAPLGA
                     LCIWLAFRLPEPGGGR"
     gene            complement(29014..29553)
                     /locus_tag="VLD60_04985"
     CDS             complement(29014..29553)
                     /locus_tag="VLD60_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HSB44345.1"
                     /translation="MTHDAEHAVPVIPWWAGGLGIGLVLIVAVGLVQPIGVSTQYVVL
                     DGVLLHTLLPAVAAQSPYLAETAQGWTLGTYQFLFVVGIPIGAFTSAWVTGRFTRRMV
                     PVEWERRFGPTPGTRLVWSFIGGFLLLFGARFGGGCTSGHMISGISQLAVSSFLFSAA
                     VFASGIVTARWLYRERERG"
     gene            complement(29683..30534)
                     /locus_tag="VLD60_04990"
     CDS             complement(29683..30534)
                     /locus_tag="VLD60_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB44346.1"
                     /translation="MDMKRAMCLCLLTGGLWWLQVQPGASSQDESPAGNQAGDSAIDA
                     VFTPPSPETIPAGLAGEQIRLGYEIVVHTQQYAKKYVGNKLNCTNCHLDGGLNPNAAS
                     FVGLAAVYPEYRARADRIMTLADRINECFERSMNGKALPPDSSKLRAVVSYIEWLSKD
                     VPRGSKVAWRGIPRINPSRQPDPGEGKNVFAKKCSFCHGTDGQGTMAAPPVWGPNSYN
                     IAAGMARVSIAASFIKGNMPRGWGWSVSDDEAFNVAAYINSQPHPDFPAKKFDWPKGG
                     KPADVPY"
     gene            complement(30581..30799)
                     /locus_tag="VLD60_04995"
     CDS             complement(30581..30799)
                     /locus_tag="VLD60_04995"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSB44347.1"
                     /translation="MTCNVGGIERPIRIVAGIGLLGVGAFAGLPVEATAAAFLFGTVA
                     LVTGAIGYCPLWSLFGVNTCPTAPPGKY"
     gene            complement(30893..31261)
                     /locus_tag="VLD60_05000"
     CDS             complement(30893..31261)
                     /locus_tag="VLD60_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44348.1"
                     /translation="MYRDALSIAGTILLVALGWAWSLTAQAGDLSRGKNVYMDVCMVC
                     HGLDGRGAASVKLNPPPADLTSAVVQGKLDAGLFKSIHDGRKNTAMGSWKYALSDEEI
                     HDVIAYVRTLGGGTGSLPKP"
     gene            complement(31528..32013)
                     /locus_tag="VLD60_05005"
     CDS             complement(31528..32013)
                     /locus_tag="VLD60_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44349.1"
                     /translation="MTQDLAFVLLNLVQVRTHNAHLLRHGEVMTVPENEVDPAHPVQV
                     ATRRQADSGIMVLSSACKVLYANQAAHHFFRRLNRKENGHLTDGAFPVSVADLFDKIL
                     KSLETRTSHRERVRLEVKRLAVEQEQQVLVQGFGIPDQLDIQRSRIVLTIQEIVPPME
                     S"
BASE COUNT         6824 a         9959 c         8803 g         6822 t
ORIGIN      
        1 gcggcgtgaa atacaccgtc gccaacaact ctaccggcaa gggcggcatc cattggtgct
       61 gaaggaccga attgccatcg tcgtggacga tgggatcgca acgggagcga cgttcttcgc
      121 ctcggtggag gccattcgcc agcaggcgcc cgggcagctc gtggcggcca ttcccgtcgg
      181 gccaagagag accatcgcca aggcgagaag tctcgtcgat gaattggtcg tgctggcgac
      241 tccagaacca ttctgggccg tgggcaatca ctatgtggat ttcacacagg tcgaggatcg
      301 cgatgtgttg gagtacttaa atctggccga cgagtctctt cgcgagtgga gagaacgaat
      361 tcattcgcct tcgcggtaac cgaaaaggaa aggacatatt catggcagcg aaagcgaaag
      421 cgacggcaag acgaggggct cgacgagatc gcctggttca agaataccag cacgatacgt
      481 ataaggtacg gggcaagttg aaggaaccga cggtgtgcag cggatgcggc gcgctctttc
      541 acaaaggacg ttggacctgg ggttccgtgc cgcgaggagc ccattcgacg acatgcccgg
      601 cctgccatcg cacgcatgat aaatatccca agggactcgt gacgatcacg gggaatttca
      661 aggaggccca gcacgaacag gtgcggggtg tcatcagaaa cacggaagcg agagagatga
      721 aagagcatcc gctgtcgcgt atcatggcaa tcgaacgccg gcccgaaggg ctggtgattt
      781 cgacgacgga tacacatctc cctcgccgga ttggcgaggc gctgacccat gcgtatcggg
      841 gcgacttgaa ctttcactac gatcgagacg aagattttat ccgggtcaat tggattcgtt
      901 gatgacgcac cggagaccgg cctctcgcgc cgggtgaagg agatccggcc atgaaggtcg
      961 gagacattat gactcgcgag gtgcagtgct gcggaccgga tacaaacctg gcggccgcag
     1021 ccaagatgat gtgggacagc gactgcggcg tggtcccgat cgtcaacgcg cagggccggg
     1081 tgatgggcat gctcacagat cgcgacatct gcatggcggc agccacgaaa accaggcccc
     1141 cggcatccat cacggcctgg gaaacctcgt cgggaaaggc catcacctgc cgtcccgatg
     1201 atgacatccg cgcggctctc gaactcatgg agcggggcaa ggtccggcgt cttccggtgg
     1261 tcgatgagga cggcatgctc caaggcatgc tgtcgatgaa cgatctcgtg ttggcagccg
     1321 gcgaacaccg gggacggagc gcgccggaac tctctgtcga tgatgtcatg cgcgcgttga
     1381 aagccatctg cgggcaccgc atccatgttg aactttaacg tgaggacttc atgaagattg
     1441 tcgtcggcgt cgactggtcc gagcaggcat ttgccaccgt gcaacaagtc ctccatctct
     1501 atcgtccgtc ggagttgact ctggttcacg gggtcgatct cggcatcttc gaatatccca
     1561 tcgtcgctca agctgccaat cttcaaggct atgacgattt ccggaattcc ctgatcgatg
     1621 cggggcgcca ggtcctggca cggaccgcca acatgctccc ctttgactcc gacacggtca
     1681 aacaagtcaa cgaggtcggc aacccggcgg agatcatcct caagacggca aacgccgttg
     1741 aggcggatct cgtggccgta ggcgcgcgcg gccacagtcg cgtgacggaa ctcgtcctcg
     1801 gcagcgtgtc tcaccgcgtg ctcatgcact ccacccgccc gacgctcatc gtcaaaggaa
     1861 cgagcaggcc gattcaacgg gtgctggtcg caatcgaagg aggggaagac gccgagcgga
     1921 tcaagcaatg gttactcgtc catcccttcg ccaatcccgt cgatctgtgt gtgctcagcg
     1981 tcgtcgtccc gatccgagtg gccgatccct acaacatcgg cggatttgaa acgtggtcgg
     2041 acagcttgaa aacgtacgcg gaagatctcg tcaagacagt cggagcagag ttgatgggga
     2101 gccggtacag catcagcacg cgcgttgcta cgggggaagt ggccgcaacc gtggcggaac
     2161 aggctaaagg catggatctc gtcgttgtgg cctcacacgg gcgaaaggga ctcgagcgct
     2221 tcctccttgg cagcgcctcc catgggatcg tccatcatgt cacgtgtccc atacttgtca
     2281 tacgctgaaa ggaattgagc atgaaaacta tcttgaccgt taccgtcctg ctggtctttg
     2341 ccgcgacctc ctgggtgtct gcgcaaggcg cgcgcggaaa tcccaagaac gggcagatcg
     2401 tgtttgaaca acattgcctt cgttgtcatg gcagcaaatt agacggaaac ggaccggacg
     2461 caaaagacct gattgtccga cccgccaatc tccaatcgca gggatcacgg gcgaagaccg
     2521 attgggaatt gctcgtgacg atcaccaacg gcgcgctatt tacccccatg cacggatttc
     2581 gcggcaaact gacggatgag cagatactcg atgtgctttc gtacatcaga tccgtggtgc
     2641 cgttcgacgc ggtgagctag cgcattgtgt ggcaccagct gacaagctgg aggggaaccc
     2701 ttcgcagtct cgtcttgtcc ctggctttcg tcttgtttat aagcggaaca ggcgttcacg
     2761 cgggcgagcc tgctccagat ctcgaagtgt tcgtacggag tggctgtccc cactgtgaag
     2821 ccgcagaaca tttcatcgat caactccggc gcgagcgccc gtcgctccac atcgtcttcc
     2881 atgacgtcgc tcaagaccca accgcgttgc agcggctcaa aacattggcc gccgaacacg
     2941 ggttcgcgaa tgtcggcgtt ccgaccttcc tgatcggcga agaagtgctc gtcggctttc
     3001 tctcggcgga aacgaccggc gctgagattc gcgccaggct ggacaagcgc gtacaaagcg
     3061 acagagcgtc ccccgtcaca gagagcatca agactacatg gttcggcgag cttcgtgtca
     3121 acgacatggg cctcccgctg tttactcttg cgatcggcct gttggatggg ttcaacccct
     3181 gcgccatgtg ggttttgctc ttcttgttat cgctgcttgt caatcttcac gatcggctca
     3241 agatggctct catcgccgga acgttcgtgg ctgtgagcgg gctactgtac tttgccttca
     3301 tggcggcctg gctcaatgtg ttcctcctgg tcggcctctc acgcgccgtg cagatcgggc
     3361 tcggcggcat cgccatcttc gtcggaacgg tccacgtgaa ggacttcttt gggtggcaac
     3421 agggtatttc gctgagcatc ccggaatccg cgaagccggg attctacgcc cgcgcccgcc
     3481 gtatactcca agccgaaaac cttgccggcg ccatggcggg cattgtcgtc ctggccggcc
     3541 ttgtgaatat gatcgagctg ctctgtaccg ccgggttccc ggctctctat acacagattc
     3601 tcacgttgca acagctgccg atctggcaat actacggtta cctcggcctg tataacctgg
     3661 cctatatatt cgacgacagt ctcatggtca ccatcgcggt aatcacgctg ggtcacagaa
     3721 agctgcaaga gcgggcggga cgttggctga agttgaccag cgggctggtg atggccggct
     3781 tgggatccgt cttgcttctt gaacccaagt ggcttatctg atggtgcgca catactgaac
     3841 tgcgcgtcat catggcgcag gaaggtcccg atggctggtg cttcacagaa atacggcgtc
     3901 gagtcgctaa agccgttctt ctatccacgt cgcgtcgcgg tcgtcggcgc atcccgcgag
     3961 cggaccggcg tcggacatcg aatcctcgaa actttacagg gaagcggctt caccggatcg
     4021 atcatccccg tcaatccaca tgcaaatgaa attgccgggc ttcatgccta tccttcgctg
     4081 agcgccattc ccgaccctgt ggatctcgcc gtcattgccg taccgcctca tctcgttctt
     4141 tcgatcatcg acgattgcgc cgcgaagcag gttcgggccg tggtcctcat cacggcagga
     4201 tttgcggaaa caggcggatc gggaacgtcg cttgaacagc agctgcatgt aaaagtccgt
     4261 caatacggga tacgcctcat cgggccgaat tgcttcggtt tgatgaacct cgatcccgcc
     4321 gtccgtctca acgcgaccta tacccccgca tttccaacta ctggccgggt tgccttcgcc
     4381 tcggaaagcg gtggactcgg cctcgccgtg gtgaccgcgg cgcggcggat acatctcggc
     4441 ctctccagct tcgtcagtgt cggcaaccat gcagacgtct ccatcaacga tctccttgag
     4501 tactgggagc aggacgacgc caccggggtg attctcctgt atctcgaaac catcgtggaa
     4561 cctcggcggt tcagattgct cactgagcgg gtggggaaaa agaaaccgat cgtcgtcctg
     4621 aaagcaggcc ggactctggc cggccaatcg gcggcgggat cgcacactgc ggcgctggcg
     4681 acggacgaga cggcggtcaa tgcattgttc aaacaatgcg gcgtgattca ggcaacgtcg
     4741 ctggaagact ttttggcgct ggccacagga ctgtcgaatc aacggctccc tgccggtcgc
     4801 cgcgtcggca tcctgacaaa ttccggcggc cctggcgtcc tctgtgccga tagctgtgcc
     4861 gaagaaggac tcagcttgcc ggaactcagc gagcgcacga ggtcaacact cgcgtccttt
     4921 cttcctccca cagcagccct cagaaaccct gtcgatgtga tcgggttcgc cacagaggaa
     4981 caacatgccc tggccgtcga aacgatgctg aaatcggacg agatcgacac tctcatcatc
     5041 ctgcacgtgt cggtcagagc gaatgacaac gaccccgttg ccgccggcat tattcgaggt
     5101 atcagcacgg ccaggaaagc gatcggacga aacaaaccgg tctacctttg ctggatggcg
     5161 gaaggagacc tggagcgaac attcatggtc ggtggagaaa cgatacccac ctacccgcat
     5221 cctgagatcc cggcccgcat tatcagccgc gccttaacct atgacgaccg gcgacaccaa
     5281 ccggccgggc agtcgcctcg ctacagcgat gtcgagtttg cgaagatcaa agcgatttgc
     5341 gccaaagccc tatcagagcg tggatccgga tggctcacca cagaagagac ctttgctgtt
     5401 cttgctctga tgacgttgcc catcgtgcaa ggagccgtag cggtcagtgc ggaggaagcc
     5461 gggaaactgg cgcataagat gggattccct gtcgcggtga aactcgcgtc ccgtcacatc
     5521 gtgcacaaga ccgggatcgc tgcagtgcgc ctcaatttgc agagcgagca ggaggttcga
     5581 gacgccttcc atgccattcg catgcggctg cttcaggatc gtaagctcga tgccatggaa
     5641 ggtgtgctcg ttcagccgat ggtatcaggc ggcgtggagg tgatggccgg catgacccgc
     5701 gatccactct tcggtccgtt gctcgccttc ggcctcggcg gcattcacgt cgagattctc
     5761 cgcgatatgc agttccggac ggctcccctc acggagcacg atgcaagggc catggtcgag
     5821 gagatcaaag gtcatcgact cctcaccggt taccgcggtc aaccggcagt cgatgcgaaa
     5881 gcgattgagg acgtcctgct gcgcatctct cgcctcgtcg aggagattcc agaaatcagt
     5941 gaactcgatc tcaatccgat tgttgttctt cctgatgcac aaggttgcaa gatcgtagac
     6001 gcgaggatcc gaatcgtaaa ggatacgcag aaggtttaag gatctgttcg acaatcgcgt
     6061 tctggcctta ctcagattga ttcgtcagat tgcgccgctt tagggagtac tgcagacagg
     6121 cggctcatag gcacaatcct cccacctgag cattttctca cttggcactc ttggaggggt
     6181 tgcgcctctt cttcgtgcgg cgggaagtcc ggcgtggctt gttgaatagg gtccgcagct
     6241 cgtccttcgc agcttcgagt cgagcgcgct gcgtcccacg aagctgtcgg ccggctcgat
     6301 tgaggtaaaa ggtcaacatc gacatcgccg aacggaacgg atcagcctta cggcgatcgc
     6361 ttcgttctgc ggagcgcttg agcgagcggg caatgtcagc cgggtcatct aaggtgaaga
     6421 cgccagggtc caaatccaac gcgttgcttg tttcggttac acgttgagac cactttttgg
     6481 atttgatggg cttcaccgta gcgtttgcaa ccaggccctg atatcggcca tctgctgctc
     6541 ggtcacggca tctttcaacg gcggcatcac atgtctcccc tcgtacacgg tcttccagaa
     6601 tgcctgctcg ttcgacagca cgggattacc cgttaatcga gggccgatgc ctcctgacgc
     6661 tcgttggccg tgacagacga cgcaactcgc gttgtagagt ttctcgccgc gtgtatgatc
     6721 accggcggga cgcctcgaag ccatcccgct tggactatct ttgtcagtgc cctgcgagcc
     6781 gccctcgctt gtcagagcga aatgaccatc cccgttgatg gaagctccta gaaacaacac
     6841 tgagacggca cagcaaatca agaatgtttg catgggcatg gctccatttc tcctccttca
     6901 ttacggaggc actgtgtcat ttgcgtgtcg cggaaccttc gctccggaga aaaaccacac
     6961 gattcaacgc atctgagagg tccttcgccg gtatcgtcgg ccacgagact cgcttcacga
     7021 tcagatttat tgcgacttca gttgttcagc acttaacatg atcaattcga tctcctccgt
     7081 aatctcttcc tgacggagaa tgttcctccg gagtcgcagc tcggaagttt ccttttcgag
     7141 gcgcttcagg gctccgtcca gatgcgtgag cctttcgcgg ctctccgcca tcagcgccga
     7201 gtagaagatc tcgtgcaaaa tggcaaataa atagagatcg accagttcgg caataaaaac
     7261 ccgaggtgga agtgtcagga gtggagccgt agagcccccc gtctcaacgc gcgggattgt
     7321 ttcaaacggc cgcaatggtt ggatcgtaat accgtccgta ccggcgacac gatagaccgc
     7381 ggtcatcacg aacgtccgac tgtcctgccg ttcttgccgg aggtcatgaa tgcgctggat
     7441 gatctcaacc aacacgcgcg ggatttcctc cgaaacggtc ggcccatcga tggcggcaac
     7501 cagacgagga tcaccggcca tacggcccga tagttttcgt cccacgacca ccatcagggg
     7561 ctccttctcg cccgccgtgt gaaggtgtcg ctccaactcg gacaacaatc gcccattgta
     7621 atcgccacag aatccccgct ccgacccgat taccagatac agcggtgtcc cgcctcggtc
     7681 ccgaggcagg gcttcgggat ggaaggcgag aaagtctctg gccgcggttt ccatgctcac
     7741 gaccacccgt tgctgggcgg aaaggaggcg ggtcagttta tggatctcca tcatcgccaa
     7801 gttgttcatg gcgctcataa tctcgctgat ctcaccgagc gtgcgaatgt ggtcatcgat
     7861 ctcatgccgt ttgctcatgg ggatcgatcc ttcctgagcc aatgccccac ggcttccgtc
     7921 cattcctctt cgccggcctc caatgtgagc gggctctccc gcacgtgctt ggccagatcc
     7981 tcgagtaaag cagggatcga atccggagga accttgtcga atagcccctg attgaaccct
     8041 gtcagccagg caagatgaaa ctcgatggcc agaggagaca atcggtcctg cttcagtaat
     8101 tcccgcaaga cacggccacg ggcaattttc gcttccatcg acgcctcgag gcgcgacccg
     8161 aaccgcgtaa acacctccaa ctcgaggaat tggagataat ccagcttcat gcgtccggct
     8221 ttttctttga tgcgatgatg ctggcctttc ccgccgacac gtgacacaga ttttgtcacg
     8281 tcgatggcgg gaagaatgcc ggatgcgaag agccgtttat cgaaataaat ctgtccatcg
     8341 gtgatcgaaa tcaaatttgt gggaatatag gcggcgattt ctccctcctt cgtctccacg
     8401 atcggcaaag ccgtcatgct gccgccgcca tgggccgggg cgagacatgt cgagcgttcg
     8461 agtagacgcg agtggagaaa aaagatgtcg cccggatagg cctcgcgccc gggaggacgg
     8521 cggagcagga gcgacagctc gcgataggct tccgcatgcg tcgtcagatc gtcgtaaatc
     8581 accaacgtgt cgctaccttg ccacatccag tgctcagcca ccgcgcaccc agcgaacgga
     8641 gccagatact tgagaccggg caacgacgtg gcctccgcga cgacgagggc cgtatggtcc
     8701 aacgcaccgt actgccggag gatctcgatc gtgtcgacga cgctggaccg cttctgtccg
     8761 accatcacat agacacagcg cacgcctcgg ccgcgctgat tgatgaccgc atcgatggcc
     8821 aaggagcttt tcccgagccc attgtcaccg ataatcaatt ggcgttggcc cttgccgatc
     8881 ggaatcagcg tatccacgat cttgctgcct gtatacagcg gttcatggac gaagtcgcgg
     8941 gccgtaatcg gcggcgagag aatatccagc agaccactgg ccgaacagtc cggccggtcc
     9001 aatccatcga gtgggttccc caagggatcg atgacccgcc cgatcagcgc atctccggtc
     9061 ggaacgccca tgcgccggcc ggacagatgt gcgatcgtac cggcagtcaa tcggctggtc
     9121 tgctcgagga ggatcgcgcc gacaacatgc gtatcgaggt agaacaccat cgcgcgactg
     9181 ccgtcttcga gatccaagat ctcatccatc gcggcagagg gtaagccctc gatccaggcg
     9241 atgccatctc cgaccgcgac gacagtcccc tgttcgtcca cccgcaggcc gaaccgataa
     9301 tcgcgcagcc acgcggcctg tcctgccaac agttgattgg gtgacaatga ttggttaggc
     9361 atggcgtgag gattcgacaa agaagttcat ttcatcctgc acgttggcgc ggagcatcca
     9421 ggatccaaaa ctgatccgta agccggcgat gagagtcctg tcttcaaaga actcgcagat
     9481 gatgtcgcgt cccgctaccg ttcggcaggc ttgggagagc atttcccgtt gggccgtatt
     9541 gagcggatag gcgctggtga gtttcatcgg ctgccctgtc ttggccgagg tagcccggat
     9601 cacctgcatt tggtcgtcag gcaagtggga aaggtcctct gtgaccatgt cgataatctt
     9661 ccgttccaac tccggccccc caagtcgtga caggaggcga ctcacgaacg ttccgcctag
     9721 cgcgatcgcg ctggcctccg ctctctgcgt gaagtccttc agccgacgct gttccactgc
     9781 cgcggctttt tgtcgttcct gatcgagttc ggcacgaaga tcttccagca atcgattccg
     9841 ctccaccctg atctcgtcat gcagctgtgt ccgggccttc tcccgttcct gttcccattc
     9901 tgtgaggcga ttctcatatt gactttgaag agcctgcgca ttgctccgcg tggtttctgc
     9961 ttccgccaaa gtcttttgaa tggccgcctt acgctggaca atcacgtcca tcaccggtcg
    10021 atagagaaac cggttgagaa tccagatgag aacaacaaag ttgacgagtt caagtatgaa
    10081 tgttgtccaa tccagttcca cggatcaccc tctcccgttc acgcacgggc agcagtagaa
    10141 cgcgttgacg acttcttcgt cacggcttca gcatgtattc gagcaacgga ttcctgaaca
    10201 gaatgatgag aacgatgacc agacagtaga tcgccaacga ttcaatcatg gccaggccga
    10261 tgaacagggc ccgcgtaatc gagcgctccg cttcaggctg ccgtgccagg gcttccaacg
    10321 cctggctgat agctcgtccc atcgccatcg caggaatcat ggttccgatg gcaatggcca
    10381 acgcggcaac gaccgtcgag gcgagggcaa accagctgat atcacgcatg attactcctg
    10441 tgcttgaggt ggttgcgatt gttgcgagtg gatcgcgccg gcgatgtaga tcaacgcgag
    10501 cataccaaag atataggcct ggatcagcgc ttcaacgatg tgcaacatca acaaggggat
    10561 cggcaccaga aagcccgcga cgagaagcac cagcagcgcg gccatctcga ggctcatgat
    10621 gttgccgaac agccggatgg cgagggccag agtgcgggtg agttcgctca tgaggtggaa
    10681 cgggagcatc agcggcatcg gagccagata gtgacgcgcg tacgctttga agccctccgc
    10741 acggatcccg aaccagtgaa ccgagaggaa cacgagcacg gccaatgccg cagtcgtcga
    10801 gagatcgccg gtgggggcat gaactccggg aatcagaccg gagaggttgg ccactatgat
    10861 gaataaccac agcgtggcga taaatgggag gagccgcgcc gtttgtgcag gcaacacggc
    10921 tcgaatcgcc tcatccgccg cattgagcag cccttccaac acgacttgga ccggccccgg
    10981 atcgaggctc agccgcatgg tcacgatcca ggaccccgca atgagagcca ccatgatacc
    11041 ccacgtggtg agaaccggct ctgtaaggaa gaggggtccg agctgcagcc ctttcgctct
    11101 gaacagatca gacgatccca tccgctactc ctttacattg aaatagacgc tcatggcgcc
    11161 gacacccacg ccgaggataa tcaggctcag cgtccaacgc atcgaatagc cggcggacaa
    11221 accgtccagc caccgaccga cataagcacc gacgatcacg ggcacaacaa acagcaagcc
    11281 cagggtcccc agatagacgg tctgcctgag gagggtgggc cggtcatgct ccgcttgctt
    11341 catgcggcga atctgcttgg ccacttcctc cttcaattgg ttctcgcttt tcatgacgca
    11401 gcatactccc ctcggcccat tcgccagagc cgcttgaaca tttcctcttc cagccgatgc
    11461 aggctgtctt tgatcgtgcg cagggcggtc tcctctgtga ccaactgctc ttcgagagcc
    11521 tggctgattc ggacgtactc atcgtcacga atgtatcgtc gcgcactcag atagaggttg
    11581 ttatcgacac aatagagcag gccgcgcggc acagccagga aatgccagcg gtcgtctgtg
    11641 gtccgatagc gtgcgaggcc gaaggccaac accgtcatca ttcgtgcatg atcggcgaga
    11701 atgccgaaga gacccgagtc gtcctgaccc acaaaactcg tgacctgatc gatccgctcg
    11761 tagtgcgtcg catcctgcag atgaaggaca aaggacttca tgcccgcacg cctttcatcg
    11821 atgcacgcat gtaacaccga tcttccggga catcgtcgta ctccccgcgc aggaacgcct
    11881 cacaatcgag gagcgtgtcg gccaaagaca cagaggcacc tcgaatgccg gtcagctccg
    11941 ccgtgacatg gaacggctga gtcagatatc gttggagttt tcgtgcgcgg acgacaatgc
    12001 gccggtccgt ctcggacagt tcttcaatac cgagcatggt gaggatatct tcaagttccc
    12061 gataccgcgc aaggtgctct ctgactcctt cggcaatgcg atagtgccgg tctccaagaa
    12121 catgacggtc catcagcttg ctcgtggatt gcagcgggtc cacggccgga tagattccct
    12181 tgctggcctg agcccgagtc aggatgacca gcgtgtccag gtgattcagg atgccgctca
    12241 cggcaggatc cgtcatatca tcagccggaa catagacggc ttgtacggat gtgatcgcac
    12301 ccttcgtggt ggaaataatg cgctcctgca actccgcgac ttcagtcatc aaagtcggct
    12361 ggtatcccac cgtcgccggc atccgtccca acaaaccaga gatttcactc ccggcctgta
    12421 cgaagcgaaa tacgttatcc atgagaaaca gcacatcctt ttggagtgtg tcgcggaaat
    12481 attctgcata ggtcaccgcg gccagcccaa cccggaaccg aactccagga gattcgtcca
    12541 tctggccgaa gatcatcact gtttgaggca tgaccccggc tttctgcatc tcatgccaga
    12601 gttcatggcc ctcccgaatc cgttcaccca ccccggcaaa tacggacacc ccgcgatgca
    12661 acgccacaat ggcgtgcatg aattccgtga ggagcacggt ctttccaatg ccggcaccgc
    12721 cgaataaacc ggtcttgccg ccccgaataa acggaaagag cagatccatc actttgatcc
    12781 cggtctcaag aattccgctc gcgggcttcg tttcatgcag tggagccggt tgggccaaaa
    12841 tattccgaaa ctcacgggtg gcgagcggcg ctccgccatc cagtggttta ccaaacaggt
    12901 tcaagactcg accaagagaa tcaggagaaa ccggcacatg cacgggcgcg cccgtatcga
    12961 atacgggcat gccccgacgc aatccgcccg ttcgatgcaa cgtgatggcg cgcacgttgt
    13021 gctcatcgag gtgttgatag acctcgaaga tgtaggtctc atggttcagc gcagaacgga
    13081 gcgcttggtg caatggagga agacgggtgc acgagatgtc gacgaccggt ccgtggacct
    13141 tggcaatcgt gccgatcaga ctatcgtcaa catggggtcc gttcgagttc actgctgaac
    13201 cgacgttctg cacaaggtgc ctcttgcaat cacttcatgg gatcagcgct ggaatgatgg
    13261 gtgagcacgg tgccaccggt tgatctcagc ggttgaacag gcgataccca cttatcctta
    13321 ccggaaccag acgatgacct gccgtgattg cccgtatgat ttgatatcgc tcgcatgacc
    13381 cagataactc gagaatggac gcttcttgtc tgctccaatt tctgacgagc gtctcctcca
    13441 atagtctgca gcgcgtgctc accgtttcca tacgataagc ggttcctcca gtgggatcaa
    13501 cgcatctgga tggaacggga caatcctcgg cgtaaagtcg tgagttggac gggttaacgg
    13561 gcagtccgcc ttatagacat atccgttcac cgtgccgggt atcgcttcct gccgagacat
    13621 agcgatacga gtcggtgact cggtctcgtt cgggggatct gcatacaatt ccacctgcac
    13681 tcgatggggt tcgaggtcgc cagaatagac ttgcgcttcg aaatgccagc acttctccgt
    13741 ctttgtgacc tgcatgtctc cgaaacgaac tcctccccag tcttctcgta gtcgtgcatg
    13801 ccactcttcc aattcgatgg ccagcttggc cccattggca atgcgacgct tcaatgccgc
    13861 gtatgccggc aggtaaatcg tctcaacata ttcgcgcacc atcctgttac tgctgaattg
    13921 cggtgtgaga cgtgccatgc tggctcgaat acgcttcagc cacgttgtcg gaatgcccgc
    13981 acgatcccgg tcatagaact ccgggatcac ctgttgttca agaatctcgt acaatctcgt
    14041 ggcctccacc gcatcgtact ccggctcgtg atgctcacgc ccatccccaa gcgcccaccc
    14101 gacctccggg ttgtaggcct cggcccacca tccgtccagc tccgacaagt tgagcccccc
    14161 gttgacgagg accttcatcc cgctcgtgcc gcaagcctcc cacggccgcc gcggcgtgtt
    14221 gagccacacg tcgatgccgg ccacgagata ctgagcaagt gcgatatcgt agtcctccag
    14281 aaacacgaca cgatcccaga catcggcgcg tacggcgaag cctgtcaact catgcaccag
    14341 gcgtttgcct tcctcatcat taggatgcgc tttccccgcg acgatcaact gtacgggacg
    14401 ttccggatta cacagcattc gcgccagccg atctgaatca tgcaacaaga gattgggacg
    14461 cttgtactcc gcaaaccgtc tggcaaatcc aatggtgatc gcattgggat caaggacatg
    14521 tccggcctgt cgaacgtgat ccggcgccgc acctcgcatc tgcatctggc gaaccagacg
    14581 gtggcgggcg tatcggatga gtgcctgccg ctgagatgcg cgaaaagacc acaagtcgct
    14641 gtctttgagc tgctcgatta aggaacacaa ttcatccagt cgccctagcc aacggtcctt
    14701 tccacaggta caagtccaga gggtatccgc ctcctgtgag tcccaggaag ggacatgcac
    14761 gccattggtc acatgtccga tcggcacttc atactgaggc caattggaat agagcggctg
    14821 gaaaatgtgc cggctgacgg ttccatgcag cttactgact ccattcacca caccacatcc
    14881 gcgcatcgcc aggaaagcca tgttgaaggg ctcatccccg ttgttgagat ctcgacgccc
    14941 caatgcgaga agatcctgaa tcggtacatg cgtcagctcc gcgaggtacg tcgcatacgg
    15001 acgaatcagt tcgagaggat accggtcgaa cgccgcttcg accggcgtat gggtcgtaaa
    15061 tacattgccg gctctggttg cccagagtgc cactgagaac gactgcccgg tccggcgcat
    15121 aaagctgagg gcacgtgcca gcaccgcaaa agcggcgtgc ccctcgttca gatggcagat
    15181 ttccacatcg agaccgagtt cttccagaac tcgccaaccc ccgacgccta gaaggatctc
    15241 ctgggtcagg cgcggcctca ctccacttgg gtaaagaagg gcagttgcgc cacggtcggc
    15301 agggctattg agcagatcgt tgctgtcgag caagtacagc tttgccttac caacctgcgc
    15361 ttgccaaact ctcaccagaa tcggacgccc gggcaattcc aatctaattc tgagccagcc
    15421 tccctcgcga ttttgcaccg gcgtgacagg gaggcttgtg gggtcattgt aaggaaagac
    15481 ctcctgttgc ctgccattcg gatccaagat ttgccgaaag tatccctgtt ggtataagag
    15541 cccgacccca acaaccggca ctcccaagtc gctcgctgtc ttcagatagt cgcccgccag
    15601 aatccccaac ccgccggaat agatcggcat ggcttcaccg aggccgaact ccatgctgaa
    15661 ataggcaata gtcttcagcc cgctcgctgc tcgttcccgt ccataccagc cggcgtcctg
    15721 caggtagcgc tgccgttccg cgagccattt ctgtatttcc tctttgaagc tggggtcctt
    15781 ggcaacctct tccagccgag attgcgacac attctggagg ataaagtagg gattttcggt
    15841 gcgttcccac acatcgggat ccagtgtttc ccagagccgg tcactgacgt gactccatgt
    15901 ccagcggaga tcaagcgcaa gatcagccag gccttcgagt cctgggggca acggtcgatt
    15961 aaaaaaatat gctagcatcc taccgggctc cgtcgttcag gtcccaaccg acttcgctcc
    16021 aaccactggc tggcatcaga gaggttcatc gccagctcca gcgaaagggc acgacaagga
    16081 accacttcca tcctgtgaat tttcggaagt agatgcctcc tggtcggcgc tgcttacaat
    16141 cggtgctgtt tcaaaggtcg cttgggattg cgccatatag ggcgaaaggt tttccaaaag
    16201 ctcaagagga aatgcaccgt tcgttggaga ttcgccgctg gctcatctct ttcattgaga
    16261 tgggctttta aataatcctt cgcgccgaat cgttgcgagg tatgccaatg tatccgcgag
    16321 ttcctgatcg gtcatcctcg tatccggaaa catccctgtg cccgtgtgcc cttcacgaat
    16381 gattcgtgcg cgttgcttgt tggttttcag atgcagcgtc tttggattgc gcagatcggt
    16441 cggttgtgga tggagctgcg cgatcagttc ggcggtgcca tcctccagct ggggcagctg
    16501 atctcgacgc ccgtctatgc catggcagta ataacagacg cctttcccgt tgaagacgat
    16561 ccgcccagcc tctgcgttgc cttcagcacg tgcagttgtt cgtgtcgatc gtccgccccc
    16621 aaatacatct ataagagggg tccaccaccc gaccgccaga cagatgacca tagatatgcc
    16681 agcccaagcg aatcctttct ccgcaactcc tccaaacgag gtcatgctgg ttcttcctat
    16741 tttcccctaa cttcggtcaa tgatcccgtc tcacgaagcg ggactcacta gtgctgttct
    16801 agagcttgac gtgtcgcctc cgcatgagcc agacaagcgt ctggttccac tgagggtggc
    16861 ggcacctccc accggcctcg cctcgatcca agatgcctta gcaaacactc cgccgtcgtc
    16921 aggagttcct caatgcctcg accgaccttt gccccgaaat gttctcggtc gaagattggg
    16981 atttgtaagc agacttccgc ccgataaccc cgttccgggg agtagacgat gcgatggcct
    17041 ggtggatccg gatattcaca aggcttcagg agctccaacg attggaaaaa gacacgacag
    17101 agacgacgga gatctccgat aagttcgcga tattcagcat cctcaccaat cggaaggtgg
    17161 cgcgggtcct ggaatgcggc ataaagattc aattggaagc caattttgac aacctccccg
    17221 gtaccggtgc ggacatagaa aggatcatga tcgtagcaga ctttccgtgt cgcgatcatc
    17281 tcccggaggg cttgctcgct tgggaacgcg ccgtctagat tgagcgtgat gtccgtcatg
    17341 tgtggttctc cccacaacac atgctgtacc gctcctccgg aatctccttc atattttgaa
    17401 aaagcgtttt ccgctcatcc tctgtcgtga cctgatcgat ggaggggtat agcacccgtt
    17461 cttccttcaa gttgtgggag gtcagtatgt tgagcagcgc ctgctcctca tggtcactgt
    17521 cgggattctg ctccgccacc ttccggtgga tggcttcaat ctgctccccg atctggcgat
    17581 gttcgttccg catgacaaac gtcggcccgc cttctgacat tccggttttc tcttcccaca
    17641 agggaaagag cagttcttct tcccacacaa tatgccgctg caagccgaac ttgaactctt
    17701 tgaaggcctc cgtggccttc gtgaagttat gtcgtttcaa cacctgaaat gattgaaaca
    17761 aggcatccaa tcggtcgtga tcctgctcga agaacgcgct gatcgtctct ggctcgctca
    17821 tgatgcctcc gcaataaatt atagttatga ctgctctcgc accttgtact cgagatgaca
    17881 ggccacgcag gctttcagca cgttattgaa ccgcggcatg atctgtttta agtctttcgt
    17941 gggaatgatt tgtgcgagtt cttccgcggc ttgatgatgc gccatggcca aatcatgata
    18001 ggcgcgcggg aaattctcgg gctgctgctg tgccatcgcc tgccgatgca tgaagaagcc
    18061 gaggtgtgat tcggtcaacc ctttggccag ctcgtagtcc tcctcaacca gcgcattgac
    18121 gatcacctga atggtttcca gatgctgcaa catcaccgcc cgatgttctt ttcgagccga
    18181 ggaggaaagt ggaatggcct gcctggtatc ggatgtgagg taggggttgg gccgctcttc
    18241 agtatgtcga tggtcgacat cggtgcaacc cgtgaataac acgccacaaa gagttcccac
    18301 cgccgccaca ccaaggatga tctgcttccc tctatataat ggtggagacg atcttccgga
    18361 catattgaac cactctcaat acaatcaatt agttagcgcc atcaggccgt tttttctgct
    18421 tttctgacct gctcccaata ggcgttcacc cgtccctgaa tatgcgagat cgcttccgtc
    18481 tgcaccgtcg gttcgaacag atgccggaac cgtccctgaa gcttgaggta gtcgtcaacc
    18541 ggttgcagct tgggcatgta ggtatgcacc acctccccgt tgatcgcttc cttcagcggc
    18601 cagagcccgc actcaacggc ttgtctcgcc acatccggcg tgttggccgg atcgaatagc
    18661 cagccggtcg gacaagcagc gaatgccaga aacagtttgg ggccaggata cttcgcagcg
    18721 cgcacgaact tttcctccaa atcgagcgga tagcgcggcg acaccgtcgc catatacggc
    18781 ggcttgtgtg cacgccaaat ctcgaaaata tcttttttct cctgcgtggt gcctgctgga
    18841 tgctgcacac cgcaaggaga cgtagtcgtc cgtgctccat agggcgtagc ggggcttaac
    18901 tggactccgg tgttaccgta ggcttcgttg tcgtagcaga aataatagaa gtccagtttc
    18961 cgagtaatcg cgcctgaagt ggaagaaagt gccatgtcat aggtggagcc gtctccgccc
    19021 aacacgacga ctttcacatc ctcttctttt ggtaatcggc ccttcgcgat taagacgtcg
    19081 agcgcgtctc ggacgccttg cgcgccggcc ggcgccgatc ccatcgtggt atagagccat
    19141 gacgcatcga gcggcgtaaa cgggaaggtc gctaagttgg taaagcatcc cgccgcattc
    19201 acatagacga catgactgcc gagtatcttg gatgcgagcc gcaacgattc cagtccgccg
    19261 cacccggcac agaggggcgt tccggggaac acgtgttcct cacgggggat ctgctgcagt
    19321 tctttaaaga tgtgaggttc ccagcccata ccgactctta tccgcccgtc ttgagttcat
    19381 gtgcgagata ctgcaactgc tccatctccc gatgttccgc ttctgtgtag agcagaaccg
    19441 gcccgattcc tttcccacgg actcccgctt ccgctgtctg ctcgaagatc gcacgaaact
    19501 cgccttccga aatattcttt ccccccagcc cgccaatgaa tgaacagagg gcccgtggcc
    19561 gcagggcttc gtgatagagg caggctgcaa tgtcctgaaa gagaataccg ccctgcccgg
    19621 gagccagatt ctgatcaaga accgccaccg ccctgcggcc ttgcaatgca tgacggatag
    19681 cgtcagccgg ccagggtctg atcagacgca gccgcaacaa tcccacccgc ctgccttcca
    19741 cccgcgccac gtcgacggca accttgcctt tactggcata cgcattggtc atgaccagca
    19801 catcctccgc gtcgtcgagg cgataacctt cgatcacgtc atagcggcgg ccaaagagcc
    19861 gttcgaaatc tgcggcggct tcctcatgca cttgaagcgc gttcatcgag gcgaggtgca
    19921 tctgatgccg gaagtaactg tacgtcgtcc cgcccagcac cgctgttccc agtgcatggg
    19981 gcgccgacgc tttaaatccc aaatgagccg gtcgaaatgg cggtaggaag gcacgcactt
    20041 tgttcgcatc cggcaggatc accggctcgc gcgtgaacga gaggacgaag ccgtctaagt
    20101 tgaccatgac cggcaggcag acgcgggggt cttccgcaat ccggtacgcc atgaggatcg
    20161 aatccactat ttcctggcag gtttctgtat ggatctgcac gaatccggag tcgcgtgccg
    20221 ccagcacatc attgtgatcc ggctccagtg tgatcggaga ggccagagcg cgcgagacat
    20281 tcaccaacac caatggcacc cgccacccgg caacgctgta caacacttca aacgcgtgca
    20341 gcagcccttg actggacgtc gcagtgaaga cgcgtgcgcc agtcgcagca gcggcccccg
    20401 cggccgtcat catcgaatgt tccgagtcca gggtgaggaa gcgggcattg agggctccgt
    20461 tgccacacca tttggacagc gtttcgacaa tttccgtttg cggagtaatc gggaaggacg
    20521 gaatgtagtc cacatcggcg aggcgagctc cccaggatgc cgcaaggttg ccagtcatca
    20581 tttcggccgt cacgactgcg ctccttgcgc ttcccgctcg gccgtgatgg catcggtcgg
    20641 gcaaacttcg acgcaatcga gacagccctt gcagtgcgga taatagatgg ccggcttgcc
    20701 gtccggcttg atcgtaatcg caccttccgg acaactcgca aagcaaagcc agcatccgtt
    20761 gcagagatca ggatgcagga caggccggaa cgtccgccag ccactcatcg atcggatcgc
    20821 tgagttgggg ccggctgtaa tactggcggt ccctctggtc ggtgattcgt aggtcggggg
    20881 gcgcagctgg gcggttgcag caggctggcg ggcacttgtc tccagagcca cagtctgcac
    20941 tctgtcataa cactggagcg ccaccgtttg attgcgttca atgaccgttg tcacaaggcc
    21001 cagatcggtc aactctcgcg cgatcgcctc gcggatcgat tcccggcgaa gcccgacgag
    21061 tcgtcccgct acagcaccca acaaggcact gatcgcgcct cgtttgccaa aatgctgaag
    21121 cgccatctca ttcagatcaa ggatcgtcag ccggcctggg agagaggtct gactgcgcac
    21181 atcgtcggca ctgagcgatg aattgacgaa cacaaccgtt cgttcatcga caccttccgt
    21241 cacccgcgcg ggtggatctg agatgagaga cgcgtcggcg accaccacaa gatcgggacg
    21301 ggcgatcaga ccccgctccc gaatcggctc cctggcgatc cttgtaaatg cggacaccgg
    21361 cgcaccgcga cgctcagcgc catacaccgg cgagtcctgt gcctgatagc cttcgagaaa
    21421 ggccgccgtc cctacaatgc ggctggcagt cttggcaccc tgcccccctc ggccatggaa
    21481 gcggatggct gtcacggctt gtcctcagct acaacacttc tccattttgt ctttgatggc
    21541 ttctttgccg gacttgactg cagcttcgac atccgcccgt ttttccgcca taaagtgctt
    21601 gcctcgttcg atgacctcgt cgagggcggc ccttgcttct ttggccttct caatcacctc
    21661 ttcctcggcc ttcctggcat agccctttag cacgcggcgg gtttcttctc cggacttggg
    21721 agcaagcaga atacccgcaa tggcaccggc aatggcgccg cccagaaaag cgagtgcaac
    21781 cgtttgacct gaacactttc cgttctgatt ttccatgacc tctcctccgt ggtgaataaa
    21841 ttctactcgc ctgaaaagcg aggtccgtgc cacgggtgcg caggaactgg gccggcgatt
    21901 actctgattt gacgggctgt tacggaagcg ttgaaatgaa ctggctaggt tcctggggca
    21961 ttccaggtct gatgagacaa ggtgcctgat gcaaaaggca acagtgccga tttcgcacgc
    22021 aagcggggtc cagtcttagg acaatacctc accggtcact gcagcagcct tcacctcctg
    22081 gccccatacc gccggactcc ctcatggacc ctctgcccct tccttggccc atcgcgccag
    22141 gcccatgctc cccggcgaag ctccgctcgt actggatgat ggcccagatc tcttcgtcgg
    22201 tcagttgatc gccgaacccg atcatgctcg ttcctgccga gccatgcttg atcacccaaa
    22261 agatttcgcc ttccgtgcga tggcgccaga atccatggtg gtgaaaattc ctgggggaag
    22321 gattcagcgg tgcagcgacc ggaccgtcgc ccgctccgct ctgaccgtgg cagttcatgc
    22381 aggttccttt gccctcatag agcgctttcc ctttggcgag catctcgggc gattccggga
    22441 acgggctggt gagggcacgg gcctccgcca gcttgtcggc cggcacccgc ggttgcatca
    22501 tgtgctgctc ggcagccacc gcgggaactc caagacttac caccagaatc ccgaatacac
    22561 tcttccatac cttcatcatg gttatcctct tttcagaaca atgcccatca ctcgtggcaa
    22621 tatcgagagg ttaggaaccg ccccactaag gctatcctgc tcccaaaggg gatcggagag
    22681 ccgtttggat tttcttgaga ctttggggct gacccaggac gatcaaggta tccccttctt
    22741 ccaacatgac ttgaggcccc ggattaaact caagatgacc gttacttcgt ttgatgccga
    22801 ccataatgag ccctcggtcc tgatcgagtc cgcattcaca tggggttttc ccgtcgaaac
    22861 gtgacccttt ttcaatcgtg atctcttcga tttgcaaatc caagtgctcg ctcttcgtgg
    22921 ccagttccag aaaatccacc acattcggtc taaggagtat ctgcgcgacc tgcatcccac
    22981 cgatcaaata gggagaggac acgcggttgg cccccgcgcg catgagcttc tgctcggatc
    23041 cttcctcccc ggctcgggct acaatataca aatctttgtt gagaccacga gccgtcaaca
    23101 cgatgtacag attctccacg tcggtattca caaccgatac caagcccttt gcgcgatcga
    23161 caccggcctg aacgagaacg tggtcctgtg tcgcatcccc ttccaccgca acaaacccat
    23221 cccgttgcgc cgcagccaca cgatcgggat tcttttccac tatcacgacg gcatacggct
    23281 tcgcgcgcaa ctccttacag atgtgttgtc ccattcggcc atagccacag acaatataat
    23341 gatggctgaa cgtcttcact ttacctcccg accgataccg accaaacacg tgctggagct
    23401 caccctcgag cacaaacctg gcaaaatttc ccaacacata gaaaagagtt ccgactcccg
    23461 ccagaatcag aatgagggta aaggcttgcg cggccttcga caggggatag atctccctgt
    23521 accccaccgt cgtcacggtg gttaccgtca tgtagagcgc gtcaaacgcc gaccatcctt
    23581 caatcagcac atacccggct gtgccgaatg cgataacgag cgcgaggacg cttgccgcat
    23641 ataccagctg ccgcagggga ttgcgggcat gattgttcgc ctccataccg gtccatcaac
    23701 cttgacaaca tgcaatcagg actgactccc gggtggccgg cttcatcaga aatccaattc
    23761 cgccatctgg tggtggaagc gcctgaccac gttcggatca gacgccagac gaacctgcgc
    23821 ctcttccttt cccttgtagg gcagaaggtt cagcacgtag cgcaagcttt cgaggcgcgc
    23881 cgcctgtttg tcgttggcct tgacggtaat ccaggggcag aacgtcgtgt gggttttgct
    23941 gaacatttcc tctttgtagc gggtatagga atcccacaac tcttgcgctt tttcatcgac
    24001 gggactgaat tcccactgct tgagcggatt ctgccggcgc acgtcgaatc ggtgctgcgg
    24061 ctcttccttc gaccagaatc gcaatgcgct ccccgtctgc tcgcacccat cgttggggcc
    24121 aaaccaattc gacctgcaat tgccgtaggt cctcttcata caacagcgcc ctccgtacct
    24181 cctccggatc gacgttccgg ctttcgagca actggttgaa ccctttccgc gtattgatcc
    24241 gtcgcagatc ctcctctgtc agcacgcggt cacgctcgcc gattagcccg tccgcctccc
    24301 tgccatctcc ccgacgcagg tcttgatatc cactcgatca gaacgccacg gtccggtcac
    24361 gttcagccac ggccttgata tagtcaggca tccccttgat cgacgcaccc gccaccagat
    24421 caccgctgct gatctgcctg gccacggcgc atgcgccgca cacccagatc tgcacacctg
    24481 ccgcttgcac ggcggccagc aagtccttga gaggagtcag gttgacgcca gtcacatgat
    24541 cggctgtgcc cttgcgccca agtgtcaccg cttcattcca aagccagagc accacgtcat
    24601 gcccctgctc cttggctact tttgccgcca tgaacggtag cgtggccatg gtgggatcgt
    24661 ctgtgcctcg actgccggaa atgattaagg tcgccatgat cgactccttg gacaagcaat
    24721 cagctgtcag cgatcggcca ttagcctttg gccaccagcc tgctgcagag ggctgaaagt
    24781 tgactgccta tttcgctaaa gaccgaatat agtgaatcaa ctgccagacc tgttcgtccg
    24841 gcaatccggc gaacgacatc atgccggttc ccggcgaacc gttcttaatg atccagaaca
    24901 gctggccatc gggaatattt ttcatcatcc cgccacaggt gaagtttctc ggtggcggga
    24961 tcaaggctgc acccatgaat cccctcccgt caccctgttc tccatggcac atcgcacagg
    25021 ccaccggctg cgccgtcttg aggaacaggg tcttccctgc ctgaactgtg ccgaccgatg
    25081 cagggaacgg atttgtcctg gccaagaaat cgtccggcgc cttcgccgtt ttgcgcggtt
    25141 gcacacaggc atcgccttgg cccccgctcg gagcagccgc ttcaggcgcg cccttgaagg
    25201 tcgccttcag ataggcgatc acatcggcca caccctgctg cccgatcgtc aggttccagt
    25261 agggcatgct cggcgacttc cccatcgcag caccgccgtg atttatcacg ttgtagagat
    25321 agtcgatcgg aagtttttcg aacgggatat tggcatggat cgcaggcttc ggttccaggc
    25381 cggatgcagc aggaccatcg cctttgccgg tgaacccgtg acactgcgcg caatattctt
    25441 tgtacagcac tttgcctcgg tccacgctgg ccatgccgaa ctcctgggtg gtccaaggct
    25501 cgtagtactt gaagtccggc acgccttgcg cggcgagata tccgatcact gcccgcaatt
    25561 gcgggtcggt cgcgtcagca agaaattccc cggtatggat gatgaagtct tgcggattct
    25621 gtccaaaacg gtagagccag tccatgttgt aacgctggcc ggcgttctgc aacgcggcgc
    25681 tctgcggacc gccgatgagc ttcccgttct cctcgatcgt gtggcaaccg agacaggcat
    25741 gagccttata ggccatcccg ccgaaggtca cctcgaactt gctcaccttc gacacgtcga
    25801 atgcgccgat cttgacgcga ggatctttat tgttcttttc gaagtagtcg gccatggcat
    25861 tcgcatcggc ctcgctcacg accggatgtt ttaccgagcc ttcactgagg tcccagcggt
    25921 agcccttggc gtacaaaggc gcttctttcc cggttagcca acggatgagc caaggtcgct
    25981 gatacttgct gccggcccag atgagatcgg gcgctcgcag attaaatcgt gaatccgcct
    26041 gcccctccag gcgatggcat tggacgcagg cctgctggat cagctccttc gcccggcttt
    26101 gatcgctccc gaacaacagc cgcgggaacg acatcagccc caccaaggtc agcagcgctc
    26161 cgccaagcat cactcctcgt ctcacattca tgtcacgact cctttcaatt gatcacttga
    26221 tttcctgcgc tcatctggtc gagagccagg catggatatc cgctatgtcc tgatggctca
    26281 agaccgaacc ccaggccggc attggccctc gcccatgcaa caccgtctcc caaaatagat
    26341 cttcgtgttt gaggatggga ttctttgcca gcttcggacc cataccgccc atagccccgg
    26401 ttccgtgaca ggcctggcag ttgtgctcga agatccccgc gcctctcgtg gccacgccga
    26461 ccaacggacc ggcagctgca ggttccggtg caagcggctc agccttgcgc agatcaatgt
    26521 caaggggcgc caactgatcc ggtgcgcccg ggtgataccg agccgacaga taccgcacga
    26581 gcaggtcggc ttcgtcattc gaaatctccg ccccccaatg tttcatcttc tcgaccgtcg
    26641 cttcccatcg caccctcggc aatcgctgct gggtaatgag atcggggctg tgacagacgg
    26701 aacagcgcgc aatgatgagc ccctccgcgc gcggagccag cgccgcactc accgtcgcca
    26761 gatcgtcctc ctgtgccgcc gttgtcatcg caatgaacag aagaccgccg aggagaagca
    26821 gcccgataac cgtccccttc tctcgcctca cgcctgacgt ctcaggtctc acgctcatgc
    26881 cgccaccgtg atcgtcacgc gatcccatcc gttccacaga aacccgcttg ggttccaagg
    26941 actcgcggct ggttgtacat ttcctctctc gtcggtcgcg cgacacagaa tggtgaacat
    27001 gccggcagtc ttcgccttcc agacatactg ccattgtctc caggcatagg gctgatcctc
    27061 tccgaccaac cgggtcggtt cccagttttt tccatcgtcg accgacacct caaccttcac
    27121 cactttgacc tcaccacccc aggccacgcc ttgaatgcta atggggcctg cacccactat
    27181 tgcaccctct tgcggcgcag cgatgagcga cttcaccacc atggcttcca ccggtatcat
    27241 ggcttgcccg cttggcgatc cgccaggagt cactggtgtg acgggaacgc ggtaggccgt
    27301 ctgcatgtag tagccctgcg cctcctctgc ctgcagagtg atatcagtca gccatttgat
    27361 gcaggaatcg gccatccagc ccggcgtaat gacgcgcacc ggcgcgccat gcaggagcgg
    27421 caacggccgt ccgttcattt cataggcgag aatcgtatcg ggatgccgtg ccttctcgag
    27481 cgggatactt ctgacaaaca gcgggacaga tgcgacaacg ggtcgatcgg ctccctgtaa
    27541 ctggacatgc cgtgatgcct ctttcacccc tgcctgcgag agcacgtcgc gcagacgcac
    27601 tccggtccac cgggcattcc cgactgctcc gcgctcccac tgaactccgg gtactttggg
    27661 acgatgaaag gcgcggccgt tcccgctgca ttgcaccacg gccgtgacgg tcacggcttc
    27721 catctgacgc aactctttca gcgcgaactc gcgcggacgt tccaccagcc caccgacccg
    27781 cagtttccaa accgcctccg agacgaggtc cggcacgggc ggcccaaagt ggcttctgac
    27841 gaaaaatcga tgattcggcg tgaggaatga ggtgaattcg tgaaccggcg tttcggcatc
    27901 aaagggtctg gagacgtgta ccttgagcgg accggcatct cgtgctgcgg catcctcttc
    27961 accagcctgt accggcgagc gacagccact cacggcgagg ccggccccga gtccgtagag
    28021 cagattcttc aaccaagcgc gccgggagac gttcacgtga tcctctccgt ttgttactgc
    28081 tagctcaccg catgactgtg cggattcaat ttgatggcca ccgtcgcggg agccgcgatc
    28141 gattgatgca ccgtgcagcc atgcgcgact ttcagcaatt tctctttttg ctccggcgtc
    28201 atgcgatgcg ggagatgaat caccagattg atctggccga cacgatgcgg gccctcggcc
    28261 atggcccatt ctgcttccac cgttaatcca tcgcgaggaa tgtcgtgccg cgcgcagaat
    28321 cggccgacaa aataccctac acaactggcc agcgagccaa cgaataactc gaccggactc
    28381 atcccggcat cctgcccgcc ctcttcaaac ggttgatcgg tgacgacccg gtgcttgccg
    28441 ctcgtgatgt cgtaacgcgt gccgccgtga aacgccacct ggagtttcat cgtccacctc
    28501 ctggctcagg cagtcgaaac gccaaccaga tacagagtgc gccgagcggc gccgcaatga
    28561 tgatccccgg caggcccagc gttcccggaa tcgttagcgg cccgtaccga ccgattcgca
    28621 gcaaggactc ttccaattcc gggaacataa aggcaaacat gagcgctcca agcagtccgc
    28681 caagaatcgt cgcccatgca tcgggttttc cctccgctgc ggccgctaac gccgtccccg
    28741 gacaataccc tgccaccgca aaaccgacgc cgaagatcgc tccggcgatg acaacaccgg
    28801 ggaggaacag atctttgact ttcatattcg ccaccccgat cgcgtcgagg agatttacac
    28861 cgatcagtcc aaccccgatc gccatcacga tcagtttgat catggtcaaa tctctcaatc
    28921 tcagcgcatc ggtcatcttc tggtggctgg acgcgccgcc taattgcagc accgcaccga
    28981 acaaggcacc caagaccaat cccagggcca gtgtcatcct cgctccctct cgcgatacag
    29041 ccagcgagcc gtgacgatcc cgcttgcgaa gaccgctgct gaaaacagaa acgaactgac
    29101 ggccagttgg gaaatcccgc tgatcatgtg accggaagtg cacccgcccc cgaagcgcgc
    29161 cccgaacagg agcagaaacc ctccgatgaa tgaccagacc agcctggttc ctggcgtcgg
    29221 cccgaaccgc cgttcccatt ccacaggaac cattcgtctc gtgaaccggc cggttaccca
    29281 tgcagatgta aaggccccga tcggaatgcc gaccacgaac aagaactgat acgtacccaa
    29341 cgtccacccc tgtgccgttt ccgccaaata cgggctttgc gcggcaacgg caggcaagag
    29401 ggtgtgcagt agcactccat caagaacgac gtattgtgtt gatacgccga tcggctggac
    29461 cagccctact gccacgatca acacaagccc gattcccagg ccgccggccc accagggaat
    29521 cacaggaacc gcatgttctg cgtcatgtgt catgcgtgtc tcacgagtgc gggaagggcg
    29581 agactcgcgc gaaaggcgcg actcggcttc acaagattat cggcccgtcg cgcttttccc
    29641 gccagtcccg cttgtcgtgg gagcgagata gtcttcacga tctcaatacg gcacatccgc
    29701 cggcttccca cctttgggcc aatcaaactt cttggcagga aaatcgggat gaggttggct
    29761 attgatatag gccgccacgt tgaacgcctc gtcatcggat acagaccagc cccacccacg
    29821 cggcatatta cctttgataa acgaggccgc aatgctcact ctcgccatac cggctgcaat
    29881 gttgtaagag ttcggccccc agaccggagg ggccgccatc gttccttgcc cgtcggtccc
    29941 gtggcagaag gagcatttct tcgcgaacac gttcttgccc tcgccgggat ccggctgccg
    30001 gctcggatta atcctcggta tcccgcgcca ggccaccttg ctcccacgcg gcacgtcctt
    30061 cgagagccac tcgatgtatg aaaccacagc ccgcagtttg gaactgtcag gcggaagcgc
    30121 ttttccattc atgctgcgct cgaaacattc attgatccga tccgccagcg tcatgatccg
    30181 atcagcgcga gcccggtact ccggatagac ggcagccagt ccgacaaatg atgcggcatt
    30241 cgggttcagc cccccgtcaa gatggcagtt ggtgcaattg agtttgttgc cgacatactt
    30301 ctttgcatac tgctgtgtat gcaccacaat ttcataccct aatcgaatct gttcccctgc
    30361 cagaccggca ggaatagtct caggagatgg aggagtgaac accgcatcaa tcgccgaatc
    30421 tcccgcttga tttccagccg gcgattcgtc ctgagaggat gcccccggtt gcacctgaag
    30481 ccaccagagc ccaccggtca ggaggcacaa gcacattgcc cgtttcatat ccatccctta
    30541 ctccctcgac tgcccgagca aaacagtcaa ctccgtgtca ttagtatttc ccggggggtg
    30601 cggttggaca ggtgttcacg ccaaagagcg accacaatgg acagtacccg atcgcgcccg
    30661 tcaccagcgc aaccgttcca aacaggaacg cggctgcggt cgcttcaacc ggcagccccg
    30721 caaacgcccc aacacccaac aggccgatac ctgcgacgat ccgaatcggt cgttcaatcc
    30781 cgcctacatt gcatgtcatc gttccacctc cctgttgatt gcctcaatcg atatgaggca
    30841 ctcgaccatg aaaagattcc tggaataaac aggaactttc catggatact ccctagggct
    30901 ttggaaggga gccagtcccc ccgcccaagg tgcggacgta cgcaatcaca tcgtgaatct
    30961 cttcatcgga cagcgcatac ttccacgaac ccatggcagt gttttttcgt ccgtcatgaa
    31021 tcgacttgaa gaggccagca tccaacttcc cttgtacaac ggcgctcgtg aggtctgcgg
    31081 gaggaggatt gagcttcact gatgcagcac ctcttccatc cagaccatgg cacaccatgc
    31141 agacgtccat gtagacgttc ttccccctcg agagatcgcc ggcctgcgcc gtcaaagacc
    31201 aggcccatcc taatgccacg agcagaatag ttcccgcgat agagagagca tctcgataca
    31261 tggtgtcacc cctttcctct acattaaccg gaccatgtgc cagccttatg ccgtgcagtt
    31321 atcgcaagaa aacaagcaaa aacttcgtgc tttaacaatt ggttagaaat gagacgggaa
    31381 acgctgtctt gcaatgtgga gagaaacgcg ccaacaagaa tctactcgct ggcgcaagat
    31441 gctacaggtg ggaagccaag cagttctgtg gtgctataaa gcagaagcga gaaaagagcg
    31501 agaaagcgat tgtccagcct tacatggtca ggattccata gggggaacaa tctcctgaat
    31561 cgtcaggaca atgcgcgatc gctggatatc cagttgatca ggtatcccga atccctgcac
    31621 caacacctgc tgctcttgtt ccacggccag ccgcttcacc tcgagtcgca ctcgctcgcg
    31681 atgactcgtt cgagtttcga gcgatttcag aatcttgtcg aagagatcag cgactgaaac
    31741 cggaaatgcg ccatcagtca aatgaccgtt ctcctttcga ttcagccgcc tgaagaaatg
    31801 atgcgcagct tgattcgcgt acaacacctt gcaggctgaa gagagcacca tgattcccga
    31861 gtcggcctgc cgcctcgtcg caacctggac gggatgtgcg ggatcaactt cgttctctgg
    31921 cacagtcatc acctccccat gccgcaatag atgcgcattg tgtgtcctga cttgaaccag
    31981 attgaggagc acaaacgcaa gatcttgtgt cataccgccc cgccgttctg tacggtcatc
    32041 cgaggccgtt tgaatcgacc tctatcacag ccatcataat gtggcggcgg gcgctcaggc
    32101 atgacgatcg atcatccaga tgctgactcg ttttgcagaa tggctacgct cgactggctc
    32161 caccttgctc ggaaggcggt cgcgtctgga tcatcattca tgcaatcacg gcagcaagtg
    32221 taggcaaaga taatgtcctc tggcctcagc ttgcgcagac ccatatagat ctgcaaattc
    32281 tgccaaagcc cctgggcggc ctcactcctc gtgttatctc ggactttgtc acagaagcaa
    32341 cagaccgaca gcatcgattt catcttacct aacccgtgca tgttcttgca cggtcctcct
    32401 tgtgtcag
//
LOCUS       DATIFR010000087         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_436026, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000087 DATIFR010000000
VERSION     DATIFR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436026"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VLD60_05010"
     CDS             complement(<1..302)
                     /locus_tag="VLD60_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB44350.1"
                     /translation="MRYLSMTVTGIAMAIAASVSYAESVSDKRSLTLEGAKKVIAATV
                     AEAKRVSAPGGSIAVVDDGGNLLALERLDQTFAASANIAIGKARTAALFKKPTKVF"
     gene            complement(395..880)
                     /locus_tag="VLD60_05015"
     CDS             complement(395..880)
                     /locus_tag="VLD60_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB44351.1"
                     /translation="MITKLLWSLTLTMAMVAGTVQAQLVEKTALTLEGAKQIAAVAEA
                     KAKAEGARVVIAVVDDGGNLLLLQRLDDTQVASVNVGIDKARTAAIFRRPSKVFEDQV
                     KNGRVSALALHGAVALQGGVPIIVNGKVIGAIGVSGETPSQDEDIAMAGAAIAASFTK
                     P"
     gene            complement(969..1418)
                     /locus_tag="VLD60_05020"
     CDS             complement(969..1418)
                     /locus_tag="VLD60_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44352.1"
                     /translation="MSTKTVFKMGFLSIITATGLFVASNGFAADVHTTTKFEGVKANS
                     GTASHARSGNNDTLTWSEEFKIPDTPAPHWQVVDSKGNVFLLQRLKIKGDKENRTITI
                     PSYVHDVAKVQVYCSWAEALLGEASFAKPITTASGERMHSDSMAMSR"
BASE COUNT          402 a          612 c          579 g          479 t
ORIGIN      
        1 tcgaagactt tagtgggttt cttgaacaat gccgccgtgc gtgccttacc gatcgcaatg
       61 ttcgcgcttg cggcaaaggt ctggtccaac ctctccaacg ccaggaggtt gccgccgtca
      121 tcgaccacgg cgatggaccc gcccggcgca ctgacgcgct tggcttcggc gaccgtcgcc
      181 gcaatgactt tcttggctcc ttcaagtgtg agactgcgct tgtccgatac cgattcggcg
      241 taactgacgg atgccgctat cgccatcgca atccctgtga ctgtcatact gaggtatctc
      301 atgacccttc tccttttctg ttggtatcgc ggtcagcgtt cggcaatcag cttccgacat
      361 ccattcagga gagccgatag cggaaagctg acggctacgg tttggtgaaa gaggccgcga
      421 tggccgctcc tgccatggcg atgtcttcgt cctggctcgg cgtttctcca ctgactccga
      481 tggcaccgat cactttgcca ttgacgatga tgggcacgcc gccttgcaat gccacggctc
      541 cgtgcaacgc taacgcggac acacgcccgt tcttcacctg atcctcaaac actttgctcg
      601 gtcgccggaa gatggccgcg gttctcgcct tgtcaatacc gacgttgacg ctcgcgactt
      661 gcgtgtcgtc caaacgctgc aacagcagga gattcccgcc gtcgtccacc acggcgatga
      721 ccacacgagc gccctcggcc ttggccttcg cttccgccac ggcagcgatc tgcttggctc
      781 cctccaacgt gagcgcggtt ttctccacta gttgggcctg cacggttcct gcgaccatgg
      841 ccatggtcag cgtgagactc cagagcaatt tcgtgatcat caccgtctcc tttgtttcag
      901 gcaggcgcga ggcccgggcg ggtcgggaag atgcggtcgc acctccccgc cacgaaccga
      961 gcattccatc accggctcat cgccatgcta tctgagtgca tgcgctcgcc agaggccgtg
     1021 gtgatcggct tggcaaacga ggcttcgcct aagagggctt cggcccacga gcaatagact
     1081 tgcactttgg cgacatcgtg cacataggag ggaatcgtga tcgtccgatt ctccttatcg
     1141 cccttgatct tcaaccgctg taacaggaac acattgccct ttgaatccac gacctgccaa
     1201 tgcggcgccg gggtgtcggg gatcttgaac tcctccgacc aggtcaacgt atcgttgttg
     1261 cccgatcgcg cgtgactggc cgtgccgctg ttggccttca cgccttcaaa cttcgttgtg
     1321 gtatgcacat cggccgcaaa gccgttcgac gccacgaaca atcctgtcgc tgtaattatg
     1381 gacaggaatc ccatcttgaa taccgtcttc gtactcataa cgccctccat gttgtttgat
     1441 tgaacgccgg tgctgagggg actcagctcg gcggcattga ccgcttctgc acgttggtct
     1501 gatgtattca gaagttctta cagaggaggg tgctgctgct gggggaggag tttgagcaag
     1561 acaagaaccc taagatccga gacacagaga actctatgac ctggtcaagg ctgctgaagt
     1621 acagggggca cgaaggcgac ggctagaaat cgacgatcgt gataggctct ggatcgggct
     1681 aagggcgtcg attcatttgg caagccttcc ctttattagc ctgaaacttc gtgaggcagc
     1741 gaggcttact cgctctggct ccgatttcga cgatagtgcg cggctacttt gcttcgatta
     1801 ccacagatac tcatgctgca ccaatctggg acacggatcg atggaacatg gattctccgg
     1861 ccgaattggc gggttatagc aacttgcgtt tccaaggcac gccagcgctg ttttggagaa
     1921 atggaaatat gaccatcttt gtgatgacca ccgacggtcg cttagcgcag atttttgaca
     1981 ctaaccggtg gaatcttgac tttcccgcag aactggctgg ccagggacag tagcgttttc
     2041 aggggagccc cgcagttcac ggaagggtgg cg
//
LOCUS       DATIFR010000088        20081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1584583, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000088 DATIFR010000000
VERSION     DATIFR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20081
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584583"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..158)
                     /locus_tag="VLD60_05025"
     CDS             complement(33..158)
                     /locus_tag="VLD60_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44353.1"
                     /translation="MIECRNHAFGTIVTQPVQRTSNGLLYLSMAITTGAGVWTEK"
     gene            264..2588
                     /locus_tag="VLD60_05030"
     CDS             264..2588
                     /locus_tag="VLD60_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB44354.1"
                     /translation="MSSPDLEEALRRSEEFAARLIESSRDCIKVLDLKGHLLSMNAHG
                     MTALEICDFGPLIGSPWVDFWEGQDKDQAKAAIELARQGKVGQFTGFFPTRQSNTPKW
                     WAVSVTAILDKDGQPEKLLAVSRDVTELKRAEQVLRESQVLLERQVAERNSDLAKTSA
                     ALREIVEGVEAKVGEQFFPALVQQLAKVLGVDYAFVSEFCVDRSKVRTLAFWSIDHFL
                     ENFEYAIAGTPCEKVLAGELYHCANQVADRFPHHKEELEQLGVQSYLAIPVTSVSGHV
                     LGHLAVMDRKPMVLEQLDLSVFKIFGARAGAELERTHMEAQLKDNEQRLRDLFDEAPI
                     AYVNEGLDSRFIRANRTAMKTLGITPDEVEGLYGASFIPDTPEAQRRLKEAFDSIGKG
                     IDTSGVVLELRRKDNGKPLWIQWWSRPDPSGTYTRTMFVDITDKVLMEQEKARLEAQN
                     VYLQEEIKGSHNFEELIGGSTSLKKVLKNVERVAPTDSTVLITGETGTGKELIARAIH
                     NLSPRKERPLVKVNCAAIPAGLIESELFGHEKGAFTGALTKKMGRFEVADRGTIFLDE
                     IGELPLDLQSKLLRVLQEGEFERVGGTQTFKVNVRVIAATNRDLAQLSKEGHYRPDLY
                     YRLNVFPIHLPSLREREGDIPLLVQYFVRKSAANLGKKIDRIPERMMTALQRYQWPGN
                     IRELEHVIERAVILSEGQELEPIDWLSQSNGKPGSVKVLTLEEVERQHIVDVLEQTNW
                     RVSGEKGAAKILGLNPTTLEARMKKLGIERKTKF"
     gene            2823..3092
                     /locus_tag="VLD60_05035"
     CDS             2823..3092
                     /locus_tag="VLD60_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSB44355.1"
                     /translation="MLKITKVEENGTKVLMKLEGKVMGQWAALLDGECRSFLRQKKAV
                     YLECSNVDFVDGKGIEVLNNLSRTQVTLMSAPGFVTELLQIGGRS"
     gene            3089..3799
                     /locus_tag="VLD60_05040"
     CDS             3089..3799
                     /locus_tag="VLD60_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB44356.1"
                     /translation="MNPHAFTYTESLGRTPSLNRMVPAPQPSGLAKGEDALLARLRRG
                     DEGAFDELVNRHHGALIRMAMSHVADREVAEEVVQDTWMAVIESLDRFEGRSSLRTWI
                     FGILIHKAKDRGVREKRHTTFSAFESYDDDNEEAVDPSRFQQSGEWAGHWAFPPQPWD
                     DQTPEKLLANQQAVHAMQQAIEALPTTLKEVLILRDVEGVEAKEVCELLKITETNLYV
                     RLHRARERVRVAVETALG"
     gene            3904..4176
                     /locus_tag="VLD60_05045"
     CDS             3904..4176
                     /locus_tag="VLD60_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB44357.1"
                     /translation="MKDHPSQWIQSGLTCRDVTDRVSEYLDDRLPILTKIRIGLHLAS
                     CADCRTYVSHIVLVRDTLTGLPKLAPSPINRLRLRRHFSLLHGSLT"
     gene            4328..4840
                     /locus_tag="VLD60_05050"
     CDS             4328..4840
                     /locus_tag="VLD60_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44358.1"
                     /translation="MTTALLCSGCPRVLYLDYHPSTPIKGKGPVRVAAFEYAGHPTGL
                     MRQKELESGSRNTERLYLSQNIGEFFTGALTKELAFAGYDLKPDSERVVWGTIEQFFL
                     DYVRADEQHFQIRATFSVSRNDTPTFTTSCRSDRQQVKDWMKSGLLIEQGIRDCIEEF
                     LRNAQAASAL"
     gene            4920..5444
                     /locus_tag="VLD60_05055"
     CDS             4920..5444
                     /locus_tag="VLD60_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSB44359.1"
                     /translation="MASFNRTLSLLALCLAVGCSPVPKKYLREAESGATLTTIVRSSE
                     YYQDRLVILGGVIMGEENRDGQLWLHVKNRPLDQDYRPQLPPSVDDPEGGWYWVVVGN
                     YQTFPSSYHHWGDMVLVGRMTGLASGKEPVIKMVYVRGSGVTSAHDGVWEDLVDANYI
                     PSMPAGAVGEMGQQ"
     gene            5540..6877
                     /locus_tag="VLD60_05060"
     CDS             5540..6877
                     /locus_tag="VLD60_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSB44360.1"
                     /translation="MASALLYDRLQFAFTATFHYLFPQLTMGLALLLLCVKSWALATG
                     DDHYDQVAQFWTKIFALSFAFGVVTGIPLEFQFGTNWAKFSNYAGGVIGQTLAMEGMF
                     AFFLESSFLGILLFGNNRCSRRLQWAASLMLFLGSWLSGYFILATNAWMQHPVAYTVA
                     QDGRLFVNSLSGLLTNPWLFWQYTHNMTAAVVTASFVMAALGAFYLLSGQYAAHAKTF
                     LRIGVLTGAAAAALMGFPTGHGNARQVFEHQPIKGAAFEGLFKTERGAGLLLVGQPNI
                     ETMTIDNPLVVPDALSILVYDELYAEVKGLDAFPREDWPDNLPLLYYSYHVMVGLGTI
                     LVSLMGLALLWLWRGRLFTARWLLWLLMLSAPFPYIATTAGWMTAELGRQPWLVYGLL
                     RTSDGASPLVHSGNALFTLLGFLGIYLGLGLLFLFLIADTIRHGPSERPTGHA"
     gene            6874..7902
                     /gene="cydB"
                     /locus_tag="VLD60_05065"
     CDS             6874..7902
                     /gene="cydB"
                     /locus_tag="VLD60_05065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HSB44361.1"
                     /translation="MMETFWYGAVTMMLAVYVVLDGFDFGVGMIYPFVARTDDDRRTV
                     LGSIGPVWNGNEVWLLAAGGLLFFAFPKAYAAGFSGFYLALIIVLWLLITRGLALELR
                     SHVDHALWTQFWDVAFAGASSLLAIVFGAALGNLIRGVPLNRSGYFFVALWTDFMPGP
                     DPGILDWFTVLMGLTSAAVLTVHGANYLAMKTEGELRGRVRRAAWLTGLVVTVLVGMA
                     LTAVPFVQPSFRLNYAAHPIGYLFPCLGLAALVAALELRRRDWDAGAFFATSLLILSM
                     LASTAWGSYPNILIASNDPANSLTVTNATAGIYGMQIGLWWFLFGFGLIIVYQVIAHR
                     AFWGKVRH"
     gene            7996..9099
                     /locus_tag="VLD60_05070"
     CDS             7996..9099
                     /locus_tag="VLD60_05070"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB44362.1"
                     /translation="MKAVAVIPGQSDSIHLAELPKPSVHEIPNGRGVLVQVLRVGVDG
                     TDKEINAAEYGQAPPGYDFLVIGHECFGRVLEVGRNVTEFMPGDYVVPTVRRPGGSFY
                     DQVGQYDMTVEDTYFERGINLRHGYLTELFVDDPEYLVKVPKGLKDVAVLLEPTSIIE
                     KGIIQAYEAQRRFKIWRPKKAAVLGAGTVGLLAALSLRMKGLEVTSFGKQSGPSRNLD
                     LLAQLGVRYISTNDLSIREAAKRYGPFDLMFEATGYSPVVFEAMECLGKNGVLVLASV
                     TGGDREHAIPADKINLDFVLGNKLVVGTVNANREYFEAGVYDFARAQLEFPGWLPKLL
                     THPVTGLENYQEMMSTLTTERNAIKVFVNVAYE"
     gene            9188..9652
                     /locus_tag="VLD60_05075"
     CDS             9188..9652
                     /locus_tag="VLD60_05075"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HSB44363.1"
                     /translation="MIRNIRHAVPAVAVLLMASVVSSIAGEYFEKDGTAIGGYDPVAY
                     FTDMKPVKGLQEFPAEYQGSTFYFSSAANRDRFAANPAKFVPQYGGYCAFGMAKGYKA
                     AIDPGAFTVVGDKRYLNYNGTVRALWVLDIQGYVKKADQNWPELRTSSQIIH"
     gene            9758..10675
                     /locus_tag="VLD60_05080"
     CDS             9758..10675
                     /locus_tag="VLD60_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HSB44364.1"
                     /translation="MANIMDAETTIDERQDETAAFPPSSASQEQGPSLPITPSVRVER
                     LYRHRLPVRISHWLNVPCLIILIMSGLQIFNAHPALYWGDRSDRDKPLLSIRPMRTDS
                     GEMKGVTTILGHQFETTGVLGYSNGMGRAFPAWATIPSAKWLAMGRQWHLFFAWVFVI
                     NGLIFGTYIFLSRHVKKDLLPTGRDLKHIPQAIKDHIVLRHAKGEEAKHYNVLQKLAY
                     LNVIFVLAPLIVLTGLTMSPTIDTAFPSLLSIFGGRQSARTIHFIACFSFVGFIVIHV
                     LQVILTGFFNNIRSMITGYFVVKHEGAQS"
     gene            10672..11442
                     /locus_tag="VLD60_05085"
     CDS             10672..11442
                     /locus_tag="VLD60_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB44365.1"
                     /translation="MKRDTALKRRQFLKGAAGAASLVALAGCDNLTQSSWFPGILNKA
                     EKLTDRVQRAITPANALAKEYTEADLSAVFPANGNTDPGTQDYAEMAKDDFADWDVQI
                     VGLVQTPMSFSLAALKDMPARTQITRHDCVEGWSAIGKWTGIPLGDLMHKVQPLPNAR
                     YAVFHCADIDDEGTPYYESMAIQDCYHPQTILAYELNGKPLDIPHGAPLRLRFERQLG
                     YKQAKYVERIELVESLDGIGGGKGGYWEDQGYEWYAGI"
     gene            11722..12921
                     /locus_tag="VLD60_05090"
     CDS             11722..12921
                     /locus_tag="VLD60_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44366.1"
                     /translation="MSILIGGALLNLLTGCLSPIAMHRAVIEYDRTVSYVEADLLLLN
                     IARARYHRPVHFTAVSSVAATFDFRTTAGIRGGIGPATDSTERALNLEYSASVAENPT
                     ITIVPITGEEFTKRVLRPLDEDKFEFLVRQGYDINMILRLMARGIAIDDERGPIVLFN
                     APSQGDGYLEFRRRLLHLAGLDEERKLFVGPILFEESQTVRTNRPPNPDEVVAALEKG
                     FRWEGDSEGNVHTVRRKAVGRILIANYDPSRLSNEERRRLHEEAQRMPTDSILVDIRP
                     DFPGGDYPLHGSVLLRSMNAIIGFVAKSIEEEPEMMVSPDSRTKTVVRNPARTLEIEE
                     SDSKPGDYEFSVPFDGRYYSIRKYPVSQGMVPSWNQEAFAVLSNLFQMTVTDLTKYPT
                     PAIAIAK"
     gene            12978..13496
                     /locus_tag="VLD60_05095"
     CDS             12978..13496
                     /locus_tag="VLD60_05095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB44367.1"
                     /translation="MIQRILTGIGVVLIGLFITWVVANAGVGMKAPEISNQTWLNSAP
                     LHLSDLKGNVVMVEFWTFGCYNCRNVEPYVKQWHQKYGGQGFVVIGVHSPEFSHEREI
                     ERVRSYIKEHDIQFAVPIDNDFSTWNKYGNRYWPAMYLIDKQGVIRHIRIGEGGYQET
                     EQLIRNLLAELS"
     gene            13506..14504
                     /locus_tag="VLD60_05100"
     CDS             13506..14504
                     /locus_tag="VLD60_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB44368.1"
                     /translation="MPRPSRRAFLKAAGGFLLASTLPSPSLGGLLDRVFGRAEAKATK
                     PITSNEEFYLTSYRSPPTVRVNEWSLSITGLVERPRTLDYSQLLAKPTVSQIVTLECV
                     GNTVAGEFMSTAAWEGASLRSLLEESGAQTDAYDVVFRAADGYSDSIRLDRAIPGDVM
                     LAHRMNGVPLPPGHGFPVRVIVPGHYGMKSVQWLTEIEVVARDYKGYYAQKGWTDDAV
                     VKTTSRIDLPGHGSTITGLRHKVEGLAFAGTRGIRLVEISTDGGERWTPATLEAPLSP
                     ASWVFWHYDWTVPKPGRHTLLARATDGTGTLQTSIEQDPAPDGATGLHEITVTVEA"
     gene            complement(14546..15166)
                     /locus_tag="VLD60_05105"
     CDS             complement(14546..15166)
                     /locus_tag="VLD60_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44369.1"
                     /translation="MMNRTAGVVAVCICVLAGCAAKGERVDIMIPGVAAAPAATGASA
                     TGPRVVVMPFDDARSDRAHLGSRSHFWGGSSHFELPKGTVGEAAAKALVQQLNKRGWQ
                     ASLASQGGGSQPDVTISGSIQDLSVNAISKFGYTEIAAKNTMMVRVANHSDQSSIQER
                     VLGSGSDEVFWFDPEDAQQLVNEVVEKNIEKFLADTKVDGRAVRLR"
     gene            complement(15235..15861)
                     /gene="pal"
                     /locus_tag="VLD60_05110"
     CDS             complement(15235..15861)
                     /gene="pal"
                     /locus_tag="VLD60_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSB44370.1"
                     /translation="MQTRSILSSVFLGLMCLSVVVLAWGCSGKRVASSSGDASSTTKE
                     KSAPATAAAPVETIKPEGMVTVPEERRSTTETARAPLAAPSAQSEGPGSSLSGDMSAV
                     ADIFFDFDRYAIRRDAQPVLEGNAIWMKKTPGKSVLIEGHCDERGTQAYNLVLGEKRA
                     RSTKHYLEDLGIPASRLKIVSYGEMRPFCKERDEDCYQKNRRAHFVVQ"
     gene            complement(15900..16844)
                     /locus_tag="VLD60_05115"
     CDS             complement(15900..16844)
                     /locus_tag="VLD60_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB44371.1"
                     /translation="MMNTMWLRLVAMIGSAILSQMVFDPAMAYEEIAVTEGGAVTGTV
                     TLNGKVPTPKGYNLTTLPDQIYCGRISDGRGWRLFQPFDIGEGGVFRQVVVYIESISR
                     GKPFAEAKPPRIEAKDCRFLPLVNLVRDDHDVVVVNMDPAMHDIQAYETSQLGPRVLF
                     NVPLPISQRYPHEAGLSAHFAKHYEGQPITQRVHMTKGRRVFVMQCGFHAYMESWALV
                     KDHPYYAITDEQGRFSLTDIPPGTYRVVVWHPYLREAATREVTIKPKESISWDVVIAA
                     PTGRLYANQMVENPYTRYNITDEVQGSIVPTLEKQRYQ"
     gene            complement(16951..17418)
                     /locus_tag="VLD60_05120"
     CDS             complement(16951..17418)
                     /locus_tag="VLD60_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44372.1"
                     /translation="MLTEGEGAVVPVRLLVINGGCLSGQLCEMLRSLGYEVTTLSCEP
                     DALAQMAHPDGIDGILLDVHQLLAGGMKGIASIRERFPHVPVITTGGVDQLKDLRRSI
                     ELGAAEYVVTPTDQELLKRKCSHVFSGRTGSPPVRSPRLQSPQTSPHGTERKG"
     gene            complement(17978..18622)
                     /locus_tag="VLD60_05125"
     CDS             complement(17978..18622)
                     /locus_tag="VLD60_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB44373.1"
                     /translation="MARPRILLADDHALVLEGFRRILEGQYDLVGTVEDGRALLDAAR
                     ALSPDLVILDVSMPLLNGIDAASQLKKLLPKTKIIFVTMHADRDYVRSAFEAGASAYL
                     LKRSAVDELEQAIRAVMAGHSYITPLITQDVVDVFLAKEPESQPRGTGLTFRQREVLQ
                     LLAEGRTVKEIATLLDVSTRTVEFHKAQIMGQLNLHSTADLIKYALTHGFIAAS"
     gene            complement(18615..19784)
                     /locus_tag="VLD60_05130"
     CDS             complement(18615..19784)
                     /locus_tag="VLD60_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSB44374.1"
                     /translation="MRQQSHNVLLITIVGLGLGLFVLDLYLPLGIGNGVLYGGLVILS
                     FLLPNRKVPVITAAVCSVLTMADVIFSPTLEGVPLWMGVTNRLFSLTAIWLPVLFFLH
                     RRRAEEALQQAHDELEARVRERTQELAALNQALVAEIDERMGTETSLRASEAVLQASQ
                     EELKQSREDLRALAGQLLTAQEEDRRRISRDLHDDINQRLAMLSMDLRQVEKSPPGDV
                     EELRSTMRSISSRLTALSDDVRQMAYRFHPSILDDLGLVKAIRRLVDDFSARAGVKVS
                     YVHKDPTTALSAELSTCLYRVVQESLNNVSRHADASLIEVELICDEDLITLSIRDNGR
                     GFDAARPTNAGGHLGLLSMHERVRLAKGHLEVESAEGKGTHIRVEIPLMKGESHG"
BASE COUNT         4214 a         5968 c         5718 g         4181 t
ORIGIN      
        1 taggaaccaa ccggacgagc cccttggcgc gcctatttct cggtccatac cccggccccg
       61 gtggtgatcg ccatggatag atacaagagt ccgttgctgg tccgctgcac cggctgcgtg
      121 acgatggtac cgaaggcatg atttcgacac tcgatcacag cacttccgta ccgatttgcc
      181 cttagttgca gctttttccc atccgatgct acacttcgcc cctgtttcgc cttccccatc
      241 aggcccatcg caaggtgatg ccgatgtcct ctcctgacct tgaagaagcc ctcaggcgca
      301 gtgaagagtt tgccgctcgt ttgatcgaaa gcagccgcga ttgcattaaa gtgctcgatc
      361 tcaagggcca tctgctctcg atgaacgcgc acgggatgac cgcgctggaa atctgcgact
      421 tcggtccgct tatcggctcg ccctgggtgg acttctggga gggacaggac aaagaccagg
      481 caaaggcggc gatcgagttg gcgcgccagg ggaaggttgg acaatttacc ggcttcttcc
      541 caacgaggca gtcgaacact cccaaatggt gggccgtctc cgtgactgcc atcctcgaca
      601 aggacggtca gccggaaaag ctgctggccg tatcccgcga tgtgaccgaa ctgaagcggg
      661 ccgagcaggt cctgcgggaa tcccaggtgc tgctcgaacg gcaagtcgcc gaacggaata
      721 gcgacctcgc caagaccagc gcggcgcttc gtgaaatcgt ggagggggtc gaggcgaaag
      781 tcggcgagca gtttttcccc gctctcgtcc aacaactggc caaggtcctc ggcgtggact
      841 atgccttcgt gtcggagttc tgcgtcgatc gatcgaaggt ccgcaccctg gccttctggt
      901 ccatcgacca tttcctggaa aatttcgagt acgcgatcgc cggaacacct tgcgaaaaag
      961 tgctggccgg cgagctctac cattgcgcga atcaggtggc cgacaggttc ccgcaccaca
     1021 aagaggagct cgagcagctc ggcgtgcaga gttatctggc gattccggtc acgagcgtga
     1081 gcgggcatgt gctggggcat ctcgccgtga tggatcgcaa gccgatggtg ctcgagcaac
     1141 tggatctttc ggtcttcaag atcttcggag cgcgggccgg cgcggaactc gaacgcacgc
     1201 acatggaagc ccaactcaaa gacaacgagc aacggctgcg cgatctgttc gacgaggcgc
     1261 cgattgccta cgtgaacgaa ggcctggact cccggttcat ccgcgccaat cggaccgcga
     1321 tgaaaaccct cggcatcacg ccggacgaag tcgaaggcct ctacggcgcc tcgttcattc
     1381 ccgatacccc tgaagctcaa cggcggttaa aagaggcttt tgattccatc ggcaaaggca
     1441 tcgacacaag cggcgtggtg ctcgaactgc ggcgcaagga caacgggaag ccgctctgga
     1501 ttcaatggtg gtcgagaccg gacccgagcg gaacctatac acgcacgatg ttcgtcgaca
     1561 tcacggacaa agtgctgatg gagcaggaga aggcccggct cgaagcgcag aacgtctatc
     1621 tccaggaaga aattaaaggc agccataact tcgaagagtt gatcggcgga tcgacttcgc
     1681 tgaaaaaagt gctcaaaaac gtggagcggg tggcaccgac cgattcaacc gtgctcatca
     1741 ccggcgagac cggcactggt aaggagttga ttgcgcgcgc gattcacaac ctgagcccgc
     1801 ggaaggagcg gccgctcgtg aaggtcaatt gcgcggcgat tccggccggc ttgatcgaaa
     1861 gcgagctgtt cggccatgag aaaggcgcgt tcaccggcgc gctcacgaaa aaaatgggcc
     1921 gcttcgaagt agcagacagg gggacgattt tcctcgacga aatcggcgag ctgccgctcg
     1981 acctgcaatc gaagctgttg cgtgtgctgc aggaagggga attcgagcgc gtgggcggga
     2041 cacagacctt caaggtcaac gtgcgggtaa tcgccgccac gaaccgagac ctcgcacagc
     2101 tgtcgaagga gggccactat cggcctgacc tgtattaccg cctcaatgtg ttcccgattc
     2161 atctgccctc cctccgtgaa cgggagggtg acattccact tctggtgcaa tatttcgtgc
     2221 gcaaatctgc agcgaacctc gggaaaaaga tcgaccgcat tccggaacgg atgatgacgg
     2281 cgctccaacg ctaccaatgg ccgggcaaca tccgcgagtt ggaacacgtg atcgaacgcg
     2341 ccgtgatctt gagtgaagga caggagctgg aaccgatcga ctggctctcg caatcgaacg
     2401 gcaagcccgg gtcggtgaag gtgctgacgc tcgaagaagt ggaacgccag catatcgttg
     2461 atgtgctgga acagacgaac tggcgcgtca gcggcgaaaa aggcgcggcc aagatcctcg
     2521 gccttaatcc cacgacgctg gaagcgcgta tgaaaaagct cgggatcgag cggaaaacca
     2581 agttctgacc ctccctttct cacctcccct cttcattcca atattttttc ccaactaatt
     2641 gggacgctcc caatatttta ggaattaacc tccgcggccc attcgctcgt tttcgaggac
     2701 aatggggaat tctgtcattt caaatcaatc gtttacgatc gctcgcccgc ctcgcgtctg
     2761 atgtggcacc gctgttgctg aatacccagt catcagagga acggtgaaag agggatgaca
     2821 cgatgttgaa gatcacgaaa gtggaagaga acggaacgaa ggtgctcatg aagctggaag
     2881 gcaaggtgat gggccagtgg gcggcgcttc tcgacggcga atgccgctcg ttccttagac
     2941 agaagaaggc ggtctatttg gaatgttcga atgttgattt cgtcgatggc aaagggatcg
     3001 aggtgttgaa caacctgtcc cgaacgcagg tcacccttat gagcgctccc ggatttgtga
     3061 cggagctgct gcagatcgga ggacggtcat gaatccccac gcattcactt ataccgaatc
     3121 gcttggtcgc acgccgtcgc tgaatcggat ggttccggct cctcaaccgt ccggtctggc
     3181 gaagggcgag gacgccctcc tggcgaggct caggcgcggt gacgaaggcg cattcgatga
     3241 actggtgaat cgacaccatg gggccctgat ccgcatggca atgagccacg tggcggaccg
     3301 ggaagtggcc gaagaagtcg tgcaggacac atggatggcg gtgatcgaaa gcctggatcg
     3361 gttcgaaggc cggtcgtcgc tcagaacctg gatcttcggt attctgattc acaaggccaa
     3421 agatcgcggt gtgcgcgaga agcgccatac gaccttctcc gctttcgagt cctatgacga
     3481 cgacaacgaa gaggccgtcg atccttcccg tttccagcaa tcgggcgaat gggccggaca
     3541 ttgggccttc ccgccacagc cctgggacga tcagactccg gaaaaactcc tcgccaacca
     3601 acaggcggtc catgcgatgc agcaggccat cgaggcgctc ccgacgacgc tgaaagaggt
     3661 gttgatcctg cgcgacgtcg aaggggtgga agccaaagaa gtctgcgaac tcttgaagat
     3721 cacggaaacc aatctgtacg ttcggctgca ccgggccagg gaacgggtgc gcgtcgcggt
     3781 agagacggct ctcggataag atcgaggttg aggcttcggc tgagcctcaa cccgattggc
     3841 tcctgtaagg aacccacaaa tagggagacg aagggacgtc accacaattg gaggacgtcc
     3901 cgcatgaaag accatccgtc acaatggata cagagtggct tgacctgccg ggacgtcacg
     3961 gaccgggtct cggagtatct ggacgaccgc cttccgatat tgaccaaaat tcgcatcggg
     4021 ctgcacctcg catcctgcgc cgattgccgc acctacgtca gccacatcgt gttggttcgg
     4081 gatacattga ccggcctgcc gaaacttgct ccttccccca tcaatcgcct tcgcttgcgc
     4141 cggcacttct ccttgctgca cggctccctg acgtagcctg gactgctttc cccccttcgc
     4201 ccttgacaga tgagcccgtg ctccgtagtc tctccgcgca ttcccacgtt ctatcaccgt
     4261 ggtctgcggt cggcttgtgc gcatgacacc aactcttctg ccccatcgat ggatgctgac
     4321 agcgctgatg acgacagccc ttttgtgctc cggctgtccg cgggtgttgt atctggatta
     4381 tcacccttcg acaccaatca aggggaaggg gccggtacga gtcgctgcct ttgaatatgc
     4441 aggccatcca acgggactga tgaggcagaa ggagctggaa tcgggatccc ggaacactga
     4501 acgtctgtat ctgtcacaaa acatcggcga atttttcaca ggcgcgctca cgaaagaact
     4561 cgcttttgcc ggctatgacc tcaagccaga ctcggagaga gtcgtctggg gcacgatcga
     4621 acaatttttc ctcgattatg tcagggcgga tgagcagcac tttcagattc gggcgacctt
     4681 ctccgtttcg cggaatgaca cccctacgtt taccacgtcc tgccgctccg accggcagca
     4741 agtgaaggac tggatgaaga gcggcttgct cattgagcag ggtatcaggg actgcatcga
     4801 agaattcctg cgcaacgcgc aggcggcgtc tgcgttgtga agcacgacgc cgggaggatc
     4861 gctcggaatc gggacaaccg ccacatggcc cgcttccgat ccgtgggagg tatctgagca
     4921 tggcctcgtt caaccgaacc ctctccctgc tggcgctgtg cctggctgtc ggatgcagcc
     4981 cggttccaaa gaagtatctg cgtgaagcgg aatccggcgc gacgctcacg acgattgtac
     5041 ggagctcaga atattaccag gacaggctcg taatcctcgg tggcgtgatc atgggagaag
     5101 aaaaccgtga cggacagttg tggctgcacg tgaaaaatcg tccgctcgac caggattacc
     5161 gaccgcagct tccccccagc gtagacgatc cagaaggcgg atggtattgg gtcgtcgtcg
     5221 ggaactatca gacctttccc agttcctacc accattgggg cgacatggtc cttgtcggtc
     5281 gcatgacagg attggcatca ggaaaagaac ccgtcatcaa gatggtctac gtgcgaggat
     5341 cgggtgtgac gtccgctcac gacggggtct gggaagacct cgtggatgcg aattatattc
     5401 caagcatgcc ggccggcgcg gtcggggaaa tgggacaaca atagggcgcg ccgcaccacc
     5461 ggtttgttag tttttccacc cctgcccgac ccattaccac ggacccatcc gatctcagcc
     5521 tgtccacatg gggggcccca tggccagtgc gctgctctac gaccgtctgc agtttgcctt
     5581 caccgcgacc tttcactatc tcttcccaca gctgacgatg gggttggctc tgctgctgct
     5641 ctgtgtcaaa agctgggcac tggctaccgg agacgaccat tacgatcagg tcgcgcaatt
     5701 ctggaccaag atctttgctt tgagttttgc attcggcgtc gtgaccggta ttccgctgga
     5761 gtttcagttc ggcaccaact gggcgaagtt ttccaactat gccggtgggg tcatcggcca
     5821 gactctggcg atggaaggaa tgtttgcgtt tttccttgaa tcctcgttcc tcggcatctt
     5881 gctgtttggc aataaccgat gcagccggcg tctgcaatgg gctgcgtcgt tgatgctctt
     5941 tctcgggtcc tggctctccg gttatttcat cctggcgacg aacgcctgga tgcagcatcc
     6001 cgtcgcctat acggtcgccc aagacggtcg attgttcgtc aatagcctct ccggtctgct
     6061 tacgaatcca tggctcttct ggcagtacac ccacaacatg accgcagcgg ttgttaccgc
     6121 ctcgtttgtg atggctgcac tgggggcgtt ctatctgttg tccggccagt acgcagccca
     6181 tgccaagacg ttcctccgca tcggcgtatt gaccggcgcc gccgcggcgg cgctcatggg
     6241 gttcccgacc ggccacggca atgcgcggca ggtctttgaa catcagccga ttaagggagc
     6301 agccttcgaa ggactgttca agaccgagcg tggagctggt ttactgctcg tcggtcagcc
     6361 gaacattgaa acgatgacga tcgacaatcc gctggtcgtg ccggacgcgt taagtatcct
     6421 cgtctatgac gagctctatg ccgaggtgaa ggggctcgac gccttcccgc gtgaagattg
     6481 gcccgacaac ttgcccctgc tctactactc ctaccatgtc atggtcgggc tcggaacgat
     6541 tctcgtctcg ctcatggggc tggcattgtt gtggttgtgg cggggacgat tgttcacggc
     6601 ccgttggctg ctctggttgc tcatgctgtc ggcgcccttt ccctacattg cgactacggc
     6661 cggctggatg accgccgagc tgggtcgcca gccctggctc gtgtacggtt tgctccgcac
     6721 ctctgacgga gcgtcgccgt tggttcattc ggggaacgcg ctgtttacgt tgctcgggtt
     6781 tctcggaatt tatctgggac tggggctgct ctttctgttc ctgatcgctg acaccattcg
     6841 ccacgggcct tcggaacgtc cgacggggca cgcatgatgg aaaccttttg gtatggtgcc
     6901 gtgaccatga tgctggcggt ctacgtggtc ctcgacggct tcgactttgg ggtgggcatg
     6961 atctatccct tcgtcgcgag aaccgacgac gatcgtcgca cggtcttggg ctcgatcggc
     7021 ccagtgtgga acggcaatga agtgtggctg cttgccgccg gcggcctgct cttcttcgcc
     7081 tttcccaaag cctatgcggc cgggttcagc ggcttttacc tggcgctgat tatcgtcttg
     7141 tggctcttga tcacccgagg actcgcgctc gagttacgtt cgcacgtgga tcatgcgctt
     7201 tggacacagt tctgggacgt ggcatttgcg ggcgcgagtt cgttactggc gatcgtcttc
     7261 ggcgcggcct tgggcaacct gattcgcgga gtaccgctga accgaagcgg ctatttcttt
     7321 gtcgcgctct ggaccgactt catgccggga ccagacccgg gcattctcga ttggttcaca
     7381 gtcctgatgg ggctgacaag cgcagccgtt ctcaccgtgc atggggccaa ttacctggcc
     7441 atgaaaacgg agggagaatt gcgcggacga gtaagacgcg cggcctggct gaccggattg
     7501 gtggtgacag tcctggtcgg aatggcgctg accgccgttc cattcgtgca accatctttc
     7561 cggctcaact atgccgccca tccgatcgga tacctgtttc catgcctcgg actggccgcg
     7621 cttgtggcag ctctcgaact acgaaggcgg gattgggacg cgggggcatt tttcgccaca
     7681 agtctcttga tcctgtcgat gctggccagc acagcctggg ggtcttaccc gaacatcctg
     7741 attgcctcga atgatcctgc caacagcttg actgtcacca acgccaccgc cggcatctat
     7801 gggatgcaga tcggcctgtg gtggtttctc tttggattcg gcctgatcat cgtctatcaa
     7861 gttattgcac accgagcctt ctggggaaaa gttcggcact gaaacacaac ctgctgccct
     7921 gtaagattgc cctccggccc tcgactcacc ggtacagatc cactcgtcat cagcctcttc
     7981 gtaaggagca cgcgtatgaa agccgtcgcc gtgattccag gacaatccga ttcgattcat
     8041 cttgccgaac tgcccaagcc gtcggtccac gagattccga acggacgcgg tgtgctcgtg
     8101 caggtgctcc gcgtcggtgt cgacggcacg gataaagaga tcaacgccgc cgaatacggc
     8161 caggcccctc ccggatacga ctttctcgtc atcggccatg aatgtttcgg acgcgtgctg
     8221 gaagtcggcc gcaacgtgac ggaattcatg cctggcgact atgtggtacc gaccgtgcgg
     8281 cgtcccggtg gaagtttcta cgatcaagtc ggccagtacg acatgaccgt ggaagacacg
     8341 tacttcgagc gaggcatcaa ccttcgccat ggctatctca ccgaactgtt cgtcgatgac
     8401 cctgaatacc tggtgaaggt ccccaaaggc ctgaaggacg tggccgtgct gctcgaaccg
     8461 acgtccatta tcgaaaaggg gatcatccag gcctatgaag cgcaacgacg attcaagatt
     8521 tggcggccga agaaggccgc ggtgctaggc gccggcacgg tcggcctgct tgcagctctc
     8581 tcgctgcgca tgaaaggcct tgaggtcacc agcttcggga agcagagcgg gccgtcgcgg
     8641 aacctcgacc tcttagcgca actgggcgta cggtacatct cgacgaatga tctctcgatc
     8701 agggaagcag cgaagcggta cgggccgttc gatctcatgt tcgaagcgac cggatattcg
     8761 cctgtggtct tcgaggcgat ggaatgcctc ggcaagaatg gcgtgctggt cctggcgagc
     8821 gtcacgggag gcgaccgcga acatgcgatc ccggcggaca aaatcaatct ggatttcgtc
     8881 ctgggcaaca aactggttgt cgggacggtc aacgccaatc gcgaatactt cgaagcgggc
     8941 gtatacgatt tcgcgcgggc ccagctggaa ttccccggct ggctgccgaa gcttctgact
     9001 catccggtga cggggctgga aaactatcaa gagatgatgt cgacgctcac aacggaacga
     9061 aacgccataa aagtatttgt caacgtcgcc tacgagtaga agcgatcagc cttcagctgc
     9121 cagtttcgaa aagctacctt cacgctaaga gctgatggct gaaagccgaa cgcgatagag
     9181 taaaggcatg attaggaaca ttagacacgc tgtccccgcc gttgccgtcc tgctaatggc
     9241 aagtgtcgtg tcctccatcg ccggagaata cttcgagaag gacggcacgg ccatcggcgg
     9301 gtacgatccc gtcgcctatt tcaccgatat gaaaccggtg aagggtttgc aagagttccc
     9361 tgcggagtat caaggctcga cgttttactt ctcgtcagcg gccaaccgcg acaggttcgc
     9421 cgccaaccct gctaagtttg tcccgcagta tggcggctat tgcgccttcg gcatggcaaa
     9481 gggatacaaa gcggccatcg atcctggcgc gttcaccgtc gtcggcgaca aacgctatct
     9541 caactacaat gggacggtcc gtgctttatg ggtcctcgac atccaaggct atgtgaaaaa
     9601 ggccgatcag aattggcctg agctcagaac atcctctcaa attatccact agtcctcccc
     9661 gcttcccctt accgtaagaa accgcgcctc cgatcgacaa aatccctgtg ggccgggctg
     9721 ccggtccggc cgcttctttc acaccgggag aactgccatg gccaacataa tggacgcgga
     9781 gaccacgatc gacgagcgcc aggacgagac cgccgccttc cctccttcat ctgcatcaca
     9841 agagcaaggg ccttcactgc caatcactcc ttccgtcaga gtcgaacgtc tctaccgcca
     9901 tcggctcccg gttcggatca gccattggct caacgttccc tgtctcatca tcctgatcat
     9961 gagcggtctg caaattttca acgcgcatcc ggcgctctac tggggggacc gctccgatcg
    10021 cgacaagcct ctcctctcga tccgtccgat gagaactgat agcggggaga tgaaaggcgt
    10081 cacgacgatt ctcggtcacc agttcgagac gacgggcgtg ctcggttatt caaacgggat
    10141 gggccgcgcc tttcccgcct gggccacaat tccaagcgcg aagtggctgg cgatgggccg
    10201 gcaatggcat ctgttcttcg cctgggtctt cgtcatcaac ggtctgatct tcgggactta
    10261 tatcttcctc agccgtcatg tgaagaaaga cctattgccg actgggcggg acttgaaaca
    10321 catcccgcaa gcgataaagg atcacattgt attgcggcat gcgaaaggcg aggaggcaaa
    10381 acactacaac gtgcttcaga agctggccta tctcaatgtg atcttcgtcc ttgcaccact
    10441 gattgtattg accggtctca cgatgtctcc aacaatcgac acggccttcc cctcgctcct
    10501 atcgatcttc ggcggcagac agtcggctcg caccatccac ttcatcgcct gcttctcgtt
    10561 cgtcggattc atcgtcatcc acgtcttgca ggtcatcctg acggggttct tcaacaacat
    10621 ccgctcgatg atcacaggat atttcgtcgt caagcatgaa ggagctcaat catgaagcga
    10681 gataccgccc tgaagcgacg ccagttttta aagggggcag caggagccgc cagtctggtt
    10741 gccttggccg gctgtgacaa cctgacgcaa agcagttggt tccccggcat cctgaataag
    10801 gcagagaaac tgaccgatcg agtacaacgg gccattacgc ccgccaatgc gctggcgaag
    10861 gaatataccg aggcggatct ctcagccgtc tttcccgcca acggcaatac cgaccccggc
    10921 acgcaagact atgccgagat ggccaaagac gacttcgccg attgggacgt gcagatcgtc
    10981 ggcctggtac agaccccgat gagcttctcc cttgcggcgc tcaaagacat gcctgctcga
    11041 actcaaatta cacgccatga ctgcgtggaa ggctggagtg ccattggaaa atggaccggc
    11101 atcccgctcg gagatctgat gcacaaagtt cagcctctcc cgaacgccag gtacgccgtc
    11161 ttccactgcg ccgacatcga cgacgaaggt acgccatact acgagagcat ggcgatccag
    11221 gattgttatc atccacagac gattctggcg tacgaattga acggcaaacc gctcgatatt
    11281 ccccatggcg ctccgctgcg gctgcgtttc gaacggcaac tcggttacaa gcaggccaaa
    11341 tatgtggagc gtatcgagtt ggtcgagtcg ctcgacggca taggaggcgg caagggcgga
    11401 tactgggaag atcaagggta tgaatggtac gccggtatat agtccggatg gtgaaacgag
    11461 ccgccaccgg cgttctcgcg tcgctcagac cctcaacgta ccccagaggg tacgttgagg
    11521 cccatcactc gatgcggcct tgcctggggc agggcgtgtc ttggcgcgcc ggggttgggc
    11581 gggtgggaac gttgactcgt ttgaccatcc tgtgaattgt gttcctcgta aatcctgagt
    11641 caccgaaaga caagatccat gatgtgtcac tcatcgacag cctcgggtgc tcagtcgctc
    11701 gggccttgct ggaagatccg tttgagcatc cttatcggtg gagccttgct caacttattg
    11761 accggctgtc tctcgcccat cgccatgcat cgggcggtga tcgagtatga ccgcacggtg
    11821 agttatgttg aagcggatct gttgctgctg aacatcgcac gcgcccggta ccatcggccg
    11881 gtccatttca cggccgtttc gagtgtggcc gcgacgttcg acttccggac cacggcggga
    11941 atcaggggcg gaatcggtcc ggcgaccgac tcaaccgagc gggccctcaa tctcgaatac
    12001 agcgcgagcg tcgcggagaa tccgacgatc acgatcgtgc cgattaccgg cgaggaattt
    12061 accaagcgcg tcttgcggcc gttggacgaa gacaaattcg aatttctcgt ccgccagggg
    12121 tacgacatca acatgatctt gcgcctcatg gcgcgcggga ttgcgatcga cgatgaacgc
    12181 ggcccgatcg tcctcttcaa cgctccctcg cagggcgacg gctatctgga atttcgtcgc
    12241 aggttgctcc acctggccgg cttggacgaa gagcgaaaac tcttcgtcgg gccgattctc
    12301 ttcgaggaaa gccagaccgt tcgcacgaat cgcccgccca acccggacga agtcgtcgcg
    12361 gcgctggaaa aaggttttcg gtgggaaggc gacagcgaag ggaatgtgca tacggtccgg
    12421 cgtaaagcgg taggccggat cctgatcgcc aattatgatc ccagccgcct ctcgaatgaa
    12481 gagcggcgcc ggcttcatga ggaagcgcaa cgaatgccga ctgattccat cctggtcgat
    12541 attcgaccgg actttcccgg aggcgactat cccttgcacg ggtctgttct gctccgcagc
    12601 atgaacgcaa ttatcggctt tgtggccaag agcatcgaag aggagccgga gatgatggtg
    12661 tctcccgatt ccagaaccaa gactgtcgtc agaaatccgg ccaggacgct tgaaatcgaa
    12721 gaatccgact caaagccggg cgactacgaa ttctccgtgc cattcgatgg ccgctactac
    12781 tcgattcgaa agtatcccgt cagccagggg atggttccca gctggaacca ggaagccttc
    12841 gccgtcctgt cgaacctgtt ccagatgacg gttacggacc tgacgaagta cccgacgccg
    12901 gccatcgcca tcgcgaaata gatctgttgt gcgccccggc gcgaagtttc tgtcgggacg
    12961 ctaggaagga ggagccgatg atacagcgta tcctgaccgg aatcggtgtc gtattgatcg
    13021 gtttgtttat cacgtgggtc gttgcgaacg cgggagtcgg catgaaggcg cctgagatct
    13081 cgaaccagac gtggctcaat agcgcgccgc tccatttatc ggacctgaag gggaacgtgg
    13141 tgatggtcga gttctggaca ttcggctgct acaactgtcg gaatgtcgaa ccgtacgtca
    13201 agcaatggca ccagaaatat ggcggccagg gcttcgtggt catcggcgtc cattccccgg
    13261 agttctccca cgaacgtgag atcgagcggg tgcggagtta cattaaagag cacgatattc
    13321 agtttgccgt gccgatcgac aacgattttt cgacttggaa taaatacggc aaccgttact
    13381 ggccggcgat gtacttgatc gacaaacaag gcgtgattcg ccatattcgc atcggcgaag
    13441 gcggctatca ggaaacggaa cagctcatcc gcaatttgct ggccgagctt tcatagaggc
    13501 gcagcatgcc aagaccttcg cgacgggcat tcctcaaggc tgccggagga ttcctgctgg
    13561 cttccaccct accctcacca agtctgggag gccttttgga ccgcgtcttc ggtcgcgcgg
    13621 aggcgaaggc caccaagccc atcacgtcaa acgaggaatt ctatctcacc tcgtatcgga
    13681 gcccgccgac ggtgcgcgtc aatgagtggt ccctctcgat cacgggactc gttgaacgcc
    13741 cgcgcacgct ggattattcc caactgctcg cgaaaccgac ggtttcgcag atcgtcacgc
    13801 ttgaatgtgt cggcaatacg gtggcagggg aattcatgag cacggctgcc tgggaaggcg
    13861 cctctctccg atcgctcctg gaggaatcag gagcgcagac cgatgcctat gacgtggtct
    13921 ttcgcgcggc agacggctac tcggacagca tccggcttga tcgtgccata cccggcgacg
    13981 tcatgctcgc gcatagaatg aacggcgtac cgctgccgcc ggggcacgga ttccctgttc
    14041 gcgttatcgt gccgggccac tatggcatga agagcgtgca gtggctgacg gagatcgaag
    14101 tcgtcgcgcg ggactacaag ggctattatg ctcagaaggg ctggacggac gacgccgtcg
    14161 tcaagacgac gtcacgcatc gatctgccag gccatggatc aacgattacg ggacttcggc
    14221 acaaggtgga gggactcgcc ttcgccggca cgcggggcat cagactggtg gaaatcagca
    14281 ccgacggagg ggaacgctgg acgccggcaa ctctggaagc tcctctctct cctgcttcct
    14341 gggtgttctg gcactatgat tggaccgtgc ccaaaccagg ccggcataca ttgcttgccc
    14401 gcgcgacgga cggaacgggc accctgcaga cctcgatcga gcaggacccg gctcccgatg
    14461 gcgccaccgg actgcacgag atcaccgtca cagtggaagc ctgaatctct gccgcagcgg
    14521 gtggcccgcg acggcaatac tcgtgctacc gtaaacggac cgcgcgccca tcgactttcg
    14581 tgtcggccaa gaatttctcg atgttcttct cgaccacctc gttcactaac tgctgggcat
    14641 cttccgggtc gaaccaaaac acttcgtcgc tcccgctccc caacacccgc tcttgaatcg
    14701 agctctgatc gctatggttg gccacgcgta ccatcatggt gttcttggcg gcgatctcgg
    14761 tatagccaaa tttgctgatg gcgttcaccg acaaatcttg aatgctgccg gagatcgtca
    14821 catcgggttg tgaaccgccg ccttgactgg ccagacttgc ctgccaccca cgcttgttga
    14881 gctgttgcac gagagccttg gccgcagctt cgccgaccgt gcctttcggc agttcgaagt
    14941 ggctggagcc gccccagaaa tgggaacgac tgccgagatg cgcccggtcc gaccttgcat
    15001 cgtcgaatgg catgacgacg acccgaggtc cggttgcact tgcccctgtc gctgcaggag
    15061 cggcagcgac accgggaatc atgatatcga cccgctctcc tttggcggca catcccgcca
    15121 agacacagat acaaacggcc acgactcccg cagttcgatt catcatcaca agtctctccc
    15181 ctttctcgtt aaaccggcct gcacatacct caattcattc gcagcaacag cgaattactg
    15241 tacaacgaaa tgtgcccgcc gatttttttg ataacaatcc tcgtcacgct ccttgcaaaa
    15301 cggtctcatt tctccatagc tgacgatctt caggcgtgaa gcgggaatcc cgagatcctc
    15361 caggtaatgt ttcgtcgatc gggcgcgttt ttctccgagc accaagttat aggcttgcgt
    15421 gccccgttcg tcgcaatgcc cctcgatgag cacagatttg cccggcgtct ttttcatcca
    15481 gatcgcgttg ccttccagga caggctgagc atctcgtctg atggcatagc ggtcgaagtc
    15541 gaagaagatg tcggccaccg cggacatatc gcccgacaat gaactgcccg gcccttcact
    15601 ttgcgccgac ggagccgcca gcggcgcgcg cgctgtttca gtggtcgatc gccgttcctc
    15661 cggaactgtg accatgccct ccggcttaat ggtttccacg ggagctgcgg cagtcgccgg
    15721 cgcgctcttc tccttcgtcg tggaggacgc atcgccggag gatgatgcca cgcgcttccc
    15781 tgaacaaccc caggccagga ccaccacgct caggcacatc aaaccgagga atacgctgga
    15841 caggatcgaa cgagtctgca ttctgcacct ctccacggtg aattgcgcgc aggataccgt
    15901 tattgatacc gctgcttctc caacgtcggc acgatcgagc cctgtacctc gtcagtaatg
    15961 ttatagcgag tgtacggatt ctccaccatt tgatttgcat agagccggcc ggttggtgcc
    16021 gcgatcacca catcccaaga aattgattcc ttcggcttga tcgtcacctc ccgagttgca
    16081 gcctctcgta gatagggatg ccacacgacg acacgatagg tgccgggcgg aatgtccgtc
    16141 agactgaatc gtccctgctc gtctgtgatc gcgtagtaag gatggtcctt caccagcgcc
    16201 caactttcca tatacgcgtg gaacccgcat tgcatcacaa acacccgtcg tcctttggtc
    16261 atgtgcacgc gctgggtgat cggctggccc tcatagtgct tggcgaaatg ggcgctgagt
    16321 cccgcctcgt gcggatagcg ttggctgatc ggcaagggca cgttaaacaa cacccgcggt
    16381 ccaagctgcg atgtttcgta ggcttggatg tcgtgcatcg ccggatccat attcacgacc
    16441 accacatcgt gatcgtcacg caccagattc acgaggggaa gaaagcgaca gtccttcgct
    16501 tcaatgcggg gaggtttggc ctccgcaaat ggcttccccc gtgagataga ctcgatgtat
    16561 accacgacct gacgaaacac tccaccctct ccgatatcaa acggttgaaa cagccgccag
    16621 ccacgaccat cggagatccg cccgcagtag atctgatcgg gcaaggtcgt caaattgtag
    16681 cctttcggcg taggcacttt gccgttgagc gtcactgtcc cggtaaccgc gccgccttct
    16741 gtcaccgcta tttcctcgta ggccattgca gggtcaaaca ccatctgcga gaggattgcc
    16801 gacccaatca tcgccaccaa cctgagccac attgtattca tcatgttcct tcactccatc
    16861 gttcatcgtg accgaaggcc ttcagaggcc aaaccagccg ttcacctgag cttcgcgatc
    16921 actgccgggg gaggccgatc cctgaggcag ctatcccttc cgctctgtac cgtgaggact
    16981 ggtttggggg ctctgcaagc gagggcttcg aacaggaggg gagcctgtcc ggccggaaaa
    17041 tacatgcgag catttccgct tcaacaactc ttgatccgtc ggcgtcacaa catactctgc
    17101 cgctcccagc tcgatcgatc gccgaaggtc tttcagctgg tcgacacccc ccgttgtaat
    17161 cacggggaca tggggaaagc gctcgcgaat cgaagcaatc cccttcatgc cgccggccaa
    17221 aagctggtgg acatcgagca agatcccatc gattccatct ggatgcgcca tttgggcgag
    17281 cgcgtcaggc tcgcaagaca acgtcgtcac ctcgtatccg agtgaacgca gcatctcgca
    17341 cagttggccg gagagacagc cgccattaat gaccagcagt cgcacaggga ccacagcgcc
    17401 ctcgccttct gtcagcacgg gtgagtttcc cggtaatcaa caggtattga tgaacgacat
    17461 cctgttccgt cgccttctgc gaaccatatg caccgcatgg acatttcctt atccttgacc
    17521 accctcctga tcggtaaaat ggcggtcatc agaaaaacga ccccccggac tccggcgctt
    17581 cttctggcgg cgtcatggat ttattgtgcc ctctgatcga tctagcgaat cttgacaccg
    17641 atcacaggtc gtccgccagt accagacatc tgatggcatg agtgcctgtg tcgctaaata
    17701 agccgtacag gcttgccagg aatcttcgaa ctcgctcgtg gcaggtggcc gaacacgatc
    17761 gcaacaacgg catcgcagga ggactgcacg gccgaacatt tgccacggcg gaagaggttc
    17821 atctggctct agtgacgacc tcatgcgggc accttccaac gagagcagag atatagctct
    17881 tctcatatag tacgttcgtc tttgtgtggg aactcgtaaa acccgaagct gaataagaga
    17941 aacctactgg ggtggaatat tctcgactac cgcctcccta ggaggccgca atgaacccgt
    18001 gcgtcaatgc gtacttgatc aagtcggcgg tggaatggag gttcagttgc cccatgatct
    18061 gcgccttatg aaactcgacg gttctagtgg acacatcgag cagcgtcgcg atttccttca
    18121 cggttcggcc ctcggcgagg agctgcaaga cttctcgttg gcgaaatgtc agccccgtgc
    18181 cccgcggctg cgactcaggc tctttcgcca gaaagacatc gaccacatct tgagtgatca
    18241 gtggcgtaat ataggaatgg cccgccatca cagcccgaat cgcctgctcc agctcatcga
    18301 cagccgaccg cttcaataaa taggccgaag cgccggcctc aaacgccgac cggacataat
    18361 cgcggtcggc atgcatcgtc acaaagatga tcttcgtttt tggcaggagc ttcttcagtt
    18421 gcgacgcggc atcaattccg ttgagcaacg gcatcgacac atcaagaatc accaggtccg
    18481 gagaaagcgc tctcgccgca tcgagcaacg cccgcccgtc ttccaccgtg ccgacaaggt
    18541 cgtactggcc ctcgagaata cggcgaaagc cttcgaggac aagggcatgg tcgtcagcca
    18601 gcaggatgcg aggcctagcc atgagactct cccttcatca gcggaatctc gacgcgaata
    18661 tgcgtcccct tgccctcagc ggattccacc tctaagtgcc ccttcgccaa gcgcacgcgt
    18721 tcatgcatgc ttaataatcc aagatgccct cccgcattcg ttggtcgtgc ggcatcgaac
    18781 cctcggccat tgtcgcgaat cgagagggtg atgagatcct cgtcgcagat caattccacc
    18841 tcgatgagtg aagcatctgc atgccgcgag acgttgttca gactctcctg cacaactcgg
    18901 tagaggcaag tggagagctc ggcagagagc gccgtggtcg gatccttgtg gacataggat
    18961 accttcacgc cagcccgagc ggagaaatca tccactaatc ggcgaatggc tttgacgagt
    19021 cctaaatcgt caaggatcga aggatggaag cgataggcca tctggcgcac gtcatcggac
    19081 aacgccgtca gacggcttga aatcgatcgc atcgtcgagc gaagttcctc gacatcgccc
    19141 ggcggactct tttccacctg ccgcaagtcc atcgacagca tggccaagcg ctgattgatg
    19201 tcatcatgca gatcccggga gatccgacgg cgatcttcct cctgcgcggt cagcagttgt
    19261 cccgctaacg ctcgcaaatc ctcccggctt tgcttcaact cctcctggct cgcctggagc
    19321 acggcttcgc tggcccgtag agatgtctcc gtccccatcc tttcgtcgat ttccgccacg
    19381 agcgcctggt tgagcgctgc caactcctgc gtccgttccc gcaccctcgc ttcgagttca
    19441 tcatgggcct gctgaagcgc ctcctcggct cttcggcggt gaagaaagaa cagtaccggg
    19501 agccaaatcg ccgtcaggct gaacaggcga ttcgtcactc ccatccaaag cggcacccct
    19561 tcgagcgtcg ggctgaagat cacgtcggcc atggtgagca ctgaacacac ggccgcagta
    19621 attaccggaa cctttcgatt gggcaggagg aacgacagga tgacgagccc accgtacagc
    19681 acaccgttgc cgatcccgag aggcagatac aaatcgagta caaacaggcc gagccccagg
    19741 ccgacgatcg tgatcaggag gacgttatgg gattgctgtc gcatagtcgc tcgtcaaccg
    19801 ctattatcat ctggtccgtc acgtgcttca agacccgctg cctgaacctt gccttagcga
    19861 gatcatgcaa ggctagcttt atcaacggag agttgatgca cgccatgcat ggagaggacg
    19921 agcagagaag aaacgccctt attgagtcgc cttccgaagc gggggtataa gcaacacaag
    19981 ggctgcaagg gcgacgctga ctcccatgag tgtcatcgtg gctatccgca gcgtcgcatc
    20041 cgcctctttg cggacctccc ccgcaatccc tgccacgact t
//
LOCUS       DATIFR010000089         5195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_52651, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000089 DATIFR010000000
VERSION     DATIFR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52651"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1307)
                     /locus_tag="VLD60_05135"
     CDS             complement(114..1307)
                     /locus_tag="VLD60_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB44375.1"
                     /translation="METVCHVITKLELGGAQEVALYAVSHLDRTRFRPVLVAGPGGML
                     TEEARRLPGVQTIIVPSLGRRIHAVSDLFALFGLVALFLRLRPAIVHTHSSKAGILGR
                     WAAWLARVTVIIHTVHGFGITPAQPKWLRYWLMALERMTGWITTHWITVAQVDAQKGR
                     RWRLFDQNVSVIRPGIDPRPFQMPIQPALRARLREEFGAGPDNQLIGTVACLKPQKAP
                     EDFILVAKRVCDVMPEARFVLIGDGELRPRIEALIQLYRLQNRVRLTGWRRDIEMAMN
                     AFDALLLTSHWEGLPRVILEASAAGLPIVTTKVGGVDEVVTHGPTVRLCAAGDIKSLA
                     AGVGEILQQPAQRARGTAEHRAVLPKEFHIEEMVKQYQSLYNRLLFEQRQQPMPNSLG
                     PASQP"
     gene            complement(1319..2647)
                     /locus_tag="VLD60_05140"
     CDS             complement(1319..2647)
                     /locus_tag="VLD60_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB44376.1"
                     /translation="MIVIVGAGLAGLSTAYHLSGIPYRLYEREQEVGGLCRSYRKNGF
                     TFDYTGHLLHFRQPDIKALVERLLAGKLEKHGRKSFIYSHRTYTEYPFQVNTHGLPPE
                     VVRECLMGFIETLTRPASSLASKDRSFKQWILDNLGEGMAKHFMMPFNEKLWQVSLDE
                     LTSDWVSWLVPKPELKDVINGALGIKDKAFGYNPSFLYPASDGIKVLPESFLPGIDGV
                     THNMELIEVDTKRRRALFHDRLRGESREEHYGSLVSTIPVPELVRRCLDFPVHLKEAA
                     EGLRWVSVYNVNLGVSREQVSDKHWLYFPEPEYPFYRVGFPMNFSPSLGRTGCSSMYV
                     EMSHRPTEHQSTEQLINRARSGMEQAGILRPDDEIIVADVKDLHYAYVYFDRHRARAL
                     PAILAELERRGIYSIGRYGRWEHTSMEDAIGQGKQLAEQLRAQGAQPVPA"
     gene            complement(2644..3690)
                     /locus_tag="VLD60_05145"
     CDS             complement(2644..3690)
                     /locus_tag="VLD60_05145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HSB44377.1"
                     /translation="MLTLLLTFGLAFLFVLYGVPIARQAALKYGIVDAPDGQLKHQKE
                     PVPYFGGLAIYLAFLMSLAFTFEFRHDVLGIILGGTIVVMLGLIDDFGVLTPWTKLTG
                     QLLAVFVLIKSGIRIEIAALPDWLDLLLTVLWMVGLINAFNLLDIMDGLSAGVGAVSA
                     AGLLVVALLQGDQAVAFMLAALIGSLLGFLKYNWQPARIYMGDTGAMFIGLLLGAMAM
                     IGKYPSDHSLSLLTPVFILGIPIFDTLFVMYIRYQRGLPIFWGSPDHIAIRLRHWGMS
                     VRQVVATSYVATAVVAGVGLMMMTVSEDMAWVLCLGTMTVLLIATFMLKKIDVRKPVA
                     SLVASRAEEGTHAA"
     gene            complement(3693..4814)
                     /locus_tag="VLD60_05150"
     CDS             complement(3693..4814)
                     /locus_tag="VLD60_05150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB44378.1"
                     /translation="MRVVYFSKSSEEGPSSRYRIYQFLPYLERAGIQCSIRPLVGPTY
                     FTLLEIKSAALRTLGKTLYVGARFLKRIWDLVRMGRPDVVVVEDQLFPYCPPWVERLL
                     VLSGKKLVLEFDDAIYLTRFHGRKMPALLKISAAVIVGNATLSEYARRNASAVTVIPT
                     VVDTERFIPQARITRGAIEGSDGQITIVWIGLAYNFSYLEILSPALRLVQKEMGARFR
                     VISSRPAVLAGVEVEFRPWDLCNEVRDLQECQIGVMPLPDSEWARGKCGLKLLQYMAV
                     GIPAIASAVGVNQEIIKPGENGFLATSSDEWSSHLLSLCRDVGLRRRMGGAARRTVEE
                     RYSLHIWGPRLAGQYQAIGNQSRSMNVGGSVPQTFSEHS"
     gene            complement(4811..>5195)
                     /locus_tag="VLD60_05155"
     CDS             complement(4811..>5195)
                     /locus_tag="VLD60_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44379.1"
                     /translation="EGDPLSDLVKQNRLGMTVPCGNIEMLAEAIVQAADAPETRRVWK
                     NNCRATAEEFNWNRVFEPVVAWCRAPSPAKDKEHAALWRSPMELPPLGARSQWTYYPR
                     RAVHYYRAAGLNGLVAQTIQWWKRR"
BASE COUNT         1244 a         1545 c         1328 g         1078 t
ORIGIN      
        1 gcacgaaccc ttgctcgtca cagacagttt ccatgactgc cagcacttcg gatttgatgc
       61 tgcgatactg cgccttaaga tccaatagtg ggatgttcac atttgctccg tagctatggt
      121 tgtgatgccg ggcctaacga gttaggcatc ggctgctgtc gctgctcaaa aagcagccta
      181 ttatacagag attgatactg tttgaccatt tcttcaatgt gaaattcttt ggggagcacc
      241 gctctatgtt ccgctgtccc cctcgccctc tgtgccggtt gctggagaat ctctcccacc
      301 ccagccgcca ggctttttat atcccccgcc gcgcagagcc gcacggtagg cccatgcgtc
      361 acaacttcat ccacgccgcc gaccttcgtt gtgacaatgg gcaaccctgc cgcagaggcc
      421 tcaagaatca cccgtgggag accttcccaa tgcgacgtga gcaacagtgc atcgaacgcg
      481 ttcatcgcca tctcgatgtc gcgccgccat cctgtcaacc gcactcggtt ttgtaatcta
      541 tacagttgaa tgagtgcttc gatgcgcggg cgtaactccc cgtcgccaat taaaacgaac
      601 cgagcctccg gcatcacgtc gcatacccgc ttggctacca ggataaaatc ctccggggcc
      661 ttctgaggct tgagacaggc gacggtgccg atcaactggt tatcggggcc ggctccgaat
      721 tcctcgcgca acctggcccg caaagccggt tgaatcggca tctgaaatgg acggggatcg
      781 attcctggtc tgatcaccga gacattctga tcaaacagcc gccaccgcct gcctttttgg
      841 gcatcgacct gcgcgacggt gatccagtgc gtggtgatcc agcccgtcat tcgctcgagt
      901 gccatgagcc aataccgaag ccatttcggc tgcgccggcg tgatgccaaa cccatggaca
      961 gtgtggatga tgacggtaac ccgtgcaagc cacgcggccc atcgacccaa tattcccgct
     1021 ttggagctat gagtatggac gatagcagga cggagacgga gaaacagcgc gactaagccg
     1081 aagagcgcga agagatcgct tacagcatga atccggcggc cgagcgatgg gacaatgatc
     1141 gtctggacgc cgggaagacg tcgggcctcc tcggtcagca tccccccggg accagccaca
     1201 agcacaggac gaaacctcgt ccgatccaaa tgcgacaccg cgtagagagc gacttcttga
     1261 gcccctccca attcaagctt cgtgatgaca tgacagaccg tctccacggc ggccgtcctt
     1321 aggctggaac aggctgggcg ccctgtgctc gtagttgttc ggccaactgc ttgccctggc
     1381 caatggcatc ttccatcgac gtatgctccc accgcccgta ccgtccaatc gagtagatcc
     1441 cccggcgttc aagttcagcc aaaattgccg ggagcgcacg ggcacgatgg cgatcgaaat
     1501 agacataggc atagtggagg tctttgacgt cggcgacaat gatctcgtcg tccggccgca
     1561 agataccggc ctgttccata ccggatctgg ctcgattgat gagctgctct gttgactgat
     1621 gctccgtcgg acgatgagac atttcgacat acatcgagct gcaaccagtc cgccccagcg
     1681 aaggagaaaa gttcatgggg aacccgactc gatagaacgg atattccggc tcagggaagt
     1741 acagccaatg cttgtccgag acctgttcgc gggatacccc cagattcacg ttatagacgg
     1801 agacccaccg caaaccttcc gccgcctcct tcaagtggac gggaaagtcg agacagcggc
     1861 gcacaagctc gggaaccggg atcgtcgaaa ccagtgatcc gtaatgttct tcgcggctct
     1921 ccccccgtag gcggtcgtga aagagcgctc gtcgacgctt cgtatcgacc tcgatcagct
     1981 ccatgttgtg tgtgacgcca tcaatgccgg gcagaaacga ctccggcagc actttaatgc
     2041 cgtcggacgc cggatagaga aatgaggggt tgtatccgaa cgccttgtcc ttaatgccta
     2101 gcgccccatt gatcacgtcc ttcaactccg gcttcggcac cagccacgac acccaatccg
     2161 aagtcagttc gtctaacgac acttgccaga gtttttcgtt gaagggcatc ataaagtgct
     2221 tggccatccc ttcaccgaga ttatcgagaa tccactgttt gaaggaccgg tctttagatg
     2281 cgagggagga ggccggacgc gtcagcgttt cgataaagcc cattaaacac tcacggacga
     2341 cctctggcgg aagaccgtgg gtatttacct ggaacgggta ctccgtataa gtgcgatggg
     2401 aatagataaa agattttcgc ccgtgcttct cgagcttccc tgccaaaagg cgctcgacca
     2461 gggccttgat gtcgggttgg cgaaaatgaa gcaaatgccc cgtgtagtcg aaggtgaatc
     2521 cgtttttccg ataagaacga cagagtccac cgacctcctg ctcgcgttca tacagccgat
     2581 agggaatccc ggagaggtgg taggccgtgc tgagaccggc caagccggcg ccgacgatca
     2641 cgatcatgct gcgtgagttc cttcctctgc gcgagatgcg acaagcgacg ccacgggttt
     2701 tcgcacatcg atcttcttca gcatgaatgt ggcgatcaag agaactgtca tcgttccaag
     2761 acagaggacc cacgccatat cttcgctgac ggtcatcatc atgaggccga cacccgctac
     2821 caccgccgtc gccacataac tcgtggcgac aacctgacga acagacatcc cccaatgccg
     2881 caaacggatg gcgatgtgat cagggcttcc ccagaagatc ggcagtcctc gctgatatcg
     2941 aatgtacatc acaaaaagcg tatcgaatat cggaatccct aaaataaaca caggagttaa
     3001 aagagagagc gaatggtcgc tgggatactt accgatcatc gccatcgctc ccagcaggag
     3061 tccgatgaac atcgctcccg tgtcccccat gtagatccgc gccggttgcc aattgtattt
     3121 aagaaatccc aacagacttc cgatgagcgc cgccagcata aaggcaacgg cctgatcgcc
     3181 ctggagcaat gcgacgacga gcagaccggc ggcactcacg gcgccgacgc cggctgagag
     3241 gccgtccatg atatccaaaa ggttaaacgc attgatcagt ccgaccatcc ataatacggt
     3301 cagcaagaga tccagccagt caggcaaggc cgcgatctcg atcctaatcc cgctcttgat
     3361 caagacgaac accgcaagca gttgccccgt caattttgtc caaggcgtca gcactccgaa
     3421 atcgtctatg agccccagca ttacgacaat tgttccacca agaataatgc ccagaacgtc
     3481 atgacgaaac tcgaaggtaa aggccagact catcagaaat gcgagataaa tcgcgagccc
     3541 gccgaagtag ggcacgggtt ccttctggtg cttgagctgc ccgtccggcg catcgacgat
     3601 gccgtacttc agtgcagcct gccgagcaat cggcacgccg tacaagacga ataagaaagc
     3661 taacccgaac gtcagtagta gagtgagcat gatcaggaat gttcactgaa cgtttgaggc
     3721 actgaacctc caacgttcat cgatctcgat tgatttccga tggcttggta ctgtccagcc
     3781 agcctaggac cccagatatg caatgagtag cgttcttcaa cagttcttct ggcagcccca
     3841 cccatccgac gtctcagccc tacgtcacgg cacagactaa gaagatgact ggaccactcg
     3901 tctgatgatg tggcgaggaa accgttttct cccggcttga tgatttcctg gtttactcca
     3961 acggctgacg caatggccgg aattccgacg gccatatact gcaagagctt gagcccacat
     4021 ttcccccttg cccactctga gtcagggaga ggcatcaccc cgatttggca ttcttgaaga
     4081 tcccgaactt cgttgcatag atcccacggt ctaaattcca cctcaacccc agccaaaacc
     4141 gccggccggg aactgatgac tcgaaatcga gcacccattt ctttttgtac taaccgtaat
     4201 gcaggagaca ggatttcaag ataagagaag ttgtaggcca acccaatcca tacgatcgtt
     4261 atctgcccat ctgatccctc aatagctccc cttgtgatcc gtgcctgagg aataaatctc
     4321 tccgtatcca caacggtggg aatcaccgtg acagcggaag cattgcgtcg agcgtactcc
     4381 gataatgtgg catttcccac aataacagcg gcagatatct tcaaaagggc tggcatcttg
     4441 cgcccatgaa aacgagtcag atagattgca tcatcgaact ccagcacgag cttcttgccc
     4501 gacaaaacca gtagtcgctc gacccacggc gggcagtaag ggaataattg atcttctact
     4561 acgacaacat ccggcctccc catcctgaca agatcccaga tgcgcttgag aaatctggcc
     4621 cccacataaa gcgtcttccc aagtgtacgc aatgccgcac ttttgatttc cagcaacgtg
     4681 aagtaggttg gtcccacgag aggacgtatc gagcattgaa tacctgctct ttccagatac
     4741 ggcaggaact gataaattct atagcgacta ctcggcccct cctccgaact tttcgagaaa
     4801 tagacgactc tcaccgcctt ttccaccatt ggatagtttg agcgacgagg ccgtttaatc
     4861 ccgcagcacg gtaataatgc accgcccgcc gcgggtaata ggtccattgt gacctcgcac
     4921 cgagtggggg caattccata ggagagcgcc acagtgccgc atgctccttg tcctttgccg
     4981 ggctcggggc gcgacaccat gcgacgaccg gttcaaacac acgattccag ttgaactctt
     5041 ccgcggtagc gcggcaatta tttttccaaa cccgacgagt ctcaggagcg tcggcggctt
     5101 gaacaatggc ttcagcaagc atctcgatgt tgccgcaggg aaccgtcatt cccaaacggt
     5161 tctgcttgac gagatcacta aggggatcgc cctcg
//
LOCUS       DATIFR010000090         7763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1777066, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000090 DATIFR010000000
VERSION     DATIFR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777066"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VLD60_05160"
     CDS             complement(<1..729)
                     /locus_tag="VLD60_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44380.1"
                     /translation="MKRRVLVSLVLGGAVALAGAIGILESGAADVFLEATRPDFQKIP
                     LGVLGIENGGGPDWLGGRLEEVLKKDVKRSLVFELLDLPSLGIKVHDLGNGLGNGSKA
                     VFKQAVDNGVSVLVWGKAGLKKGEKEDDVNMEGFVYDSGSDEVVGGKRYAGSPSVVRL
                     MAHRFADELVFRYTGEPGIARTKIAYVSAQGSARELFVMDYDGYDARQLTADGFLNLM
                     PRWSPDRRFLVFTAYRDRNTQDIDM"
     gene            complement(726..1721)
                     /locus_tag="VLD60_05165"
     CDS             complement(726..1721)
                     /locus_tag="VLD60_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSB44381.1"
                     /translation="MVAPAHVQSALWLDQEEQDRFFARMGRTLTISLVLHCAILLVVA
                     GLRLAPHGERPLASVEVSLVSLPAPAKLVEVSKPVETAKRAEPAPAPAPAPPVKSVTP
                     AVTPAAKPVRSSVGKDILKDLELPPDAPKFGELTPAKSVAQPQPQAHTKVKVPDLPRV
                     PDVTPDPAVKIPQRTSISDELDRELAEELKKVKAFKPAEKLEIPKDVPVKPAPMQEAA
                     IPQTKAPQTQLKTSGSSGTNPYWGRIESIIKSQWEPPPIDVTGQMYSAVVKFRFYRNG
                     TVKDVVIQQTSGNSYFDMAGQRAVLKPRAFPPFPAEMSEAYQDVEMVFRVGETAG"
     gene            complement(1750..2202)
                     /locus_tag="VLD60_05170"
     CDS             complement(1750..2202)
                     /locus_tag="VLD60_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB44382.1"
                     /translation="MTFESRQRRFMAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIN
                     LPKSASNTIKPEMRTVLTIEKDQRLYLDKDPIGVAQLEQKLKILKHEHADVSLYLRAD
                     RDVPYGIVVQVMDGVKKAGIEKIGMVTEPVGPERVGGPSIPRTPSRKS"
     gene            complement(2199..2903)
                     /locus_tag="VLD60_05175"
     CDS             complement(2199..2903)
                     /locus_tag="VLD60_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSB44383.1"
                     /translation="MFQIGLTGLLGSLGAVSKIVLLLLFIFSVVSWAIIFAKWRAFRA
                     ADQEDQRFMALLAKSKDLDDLCRQVRRMESSPAAVLFEGVMDRLAGLHGQAQDGGGSG
                     SADRHIIERTAAHLSHSQLSKLESYLPFLATTGNITPFVGLLGTVMGIIDAFREIGAQ
                     GTASISAVAPGVAEALIATAAGLFTAIPAVIAYNYFLTRIRRTSFRLDTVTIELMASL
                     SASPAKAKPIPVGAQR"
     gene            complement(2978..3056)
                     /locus_tag="VLD60_05180"
     tRNA            complement(2978..3056)
                     /locus_tag="VLD60_05180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3018..3020),aa:Asp,seq:gtc)
     gene            complement(3179..3254)
                     /locus_tag="VLD60_05185"
     tRNA            complement(3179..3254)
                     /locus_tag="VLD60_05185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3219..3221),aa:Val,seq:tac)
     gene            complement(3363..4304)
                     /gene="xerD"
                     /locus_tag="VLD60_05190"
     CDS             complement(3363..4304)
                     /gene="xerD"
                     /locus_tag="VLD60_05190"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HSB44384.1"
                     /translation="MGVIRYHRVMETDPLVERYLNHLRVEGGLSRNTVEAYRRDLAKF
                     QAYLHQQSRIPIRQVTTDTVRGFLGSLNTAQLSQVSSARCLSTVRGWFRFLRQERMID
                     DNPASGVTVGHRGMRLPNTLSQREVTTLLEAPSHPGLEDQRDRVMLELLYATGLRVSE
                     LVSLQTAQVNFEVGFLRVTGKGSKQRIVPMGEPARRLLQQYVDQIRPSVLKQRLSRFV
                     FVTRRGGPLSRQAFWKNLRQRAKRAGITKRISPHVLRHSFATHLLEGGADLRSVQAML
                     GHASIATTQIYTHVERERLKEIHATYFPRQGRRKKMG"
     gene            4441..6387
                     /locus_tag="VLD60_05195"
     CDS             4441..6387
                     /locus_tag="VLD60_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HSB44385.1"
                     /translation="MPLKLATARLTFTLTFFCVGLGSPWPVFHPTVAEAAKNTPANHP
                     VLNQAKRLIDAGQAEEAITMLRQFLNTNPKQDLLDDTYLLLGAALYAAQQYGEGLKFL
                     QQLQGEFPNSEVIDRGKLMLARTHAAMGNPDLALPILANLRTLSQDDITKREAVHLSG
                     ELYFQKKEPTRAIQAWLDEIALSPEDQTDEIRAHIREVINGSFDKKMLDRIREAYPKS
                     FPGDLASLRLIDLYVGRGEDHQAARQIQRFLANFPAHPQAAKSSDLLASLQTKLKSYQ
                     FVIAAVLPLSGKLAPFANDVLSGVQLAVELNGDRAGGPSVGLLVRDFESTQASFLDDF
                     GGLLNYDRPIAVIGPLLSKNLPVMAELAERSRIPLLTPTATLPNVRRLGAFTFSTALT
                     FQLQAKRIAAFATGEQGYRRFCILHPDTAYGRELARLFAEEVRLHQGEIIAIESYKEG
                     ESDVGNQLKRMKAEDLKKYGLAVPVDPTKTGGKLTKLDKKILYTPGFDAIFIPSRASD
                     VGLITAQLNFHDMKVPILGSNGWNAPDFARTADQSIDGAVFVDGFFADSPNPNVQDFV
                     ERYKKRFQATPTLFAMQGYDAVKFVIEAVRKGATSGEAVRDYLMSQQDLPALGGPASF
                     GQDGTLNRPLFLIQVKRGRFTQVD"
     gene            6400..7134
                     /locus_tag="VLD60_05200"
     CDS             6400..7134
                     /locus_tag="VLD60_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSB44386.1"
                     /translation="MNDLPQLLFSISYKALPLLFAMVLHEYAHGWVANKCGDPTAKLQ
                     GRLTMNPLAHIDLFGTIIMPLLCLMLPGSFLLGWAKPVPVDARNMRQPRRDMALVAAA
                     GPGMNLALALVSALLLAVLLAIDPSLSIDNSSADKTESGATLFLLPIAVMALYSVLVN
                     VFLAIFNLIPIPPLDGGRILVSLLPPRPAMALVRLEPYGMLILVGLLVFDRDLRVIHT
                     ITNTFASGLSGTILSTAIGLGAGASQ"
     gene            7131..>7763
                     /gene="trpS"
                     /locus_tag="VLD60_05205"
     CDS             7131..>7763
                     /gene="trpS"
                     /locus_tag="VLD60_05205"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSB44387.1"
                     /translation="MTMSRKRVLSGMQPSGLMHLGNYLGALENWKALQDQYECYFFVA
                     DWHALSTNYADTSRIREYVRELLIDWLAAGIDPARATVFIQSRIPEHAILHLLLSMMI
                     PVSWLERNPTYKEKQEEIKEKDLSTYGFLGYPVLQAADILLYKPDFVPVGKDQLPHLE
                     LTREVARRFNDLYKKPVFPEPKEHLTKFPKVLGTDGRKMSKSYGNTINLSD"
BASE COUNT         1700 a         2383 c         2015 g         1665 t
ORIGIN      
        1 catgtcgatg tcttgagtgt tccgatcacg ataggcggta aatacgagaa accgacgatc
       61 gggagaccag cggggcatca aattcaagaa tccatcagcg gtgagttgcc gcgcgtcgta
      121 cccatcataa tccatgacga acagctctcg cgccgatccc tgggcggaca catacgcaat
      181 cttggttctt gcgataccag gctctcccgt atagcgaaac acgagttcat cggcaaagcg
      241 atgcgccatc aagcggacga cggacgggga ccccgcgtag cgttttccac cgacaacttc
      301 gtcgctcccg ctgtcataca cgaagccttc catgttgaca tcgtcttctt tctccccttt
      361 cttgagacct gctttgcccc agaccaggac cgacaccccg ttatccacgg cttgcttgaa
      421 gacggccttc gatccgttcc ccaaaccatt ccccaaatcg tgaactttga tccccaagct
      481 tggaaggtct agaagctcga acacgaggga acgcttcacg tcttttttca gcacttcttc
      541 gaggcgcccg ccgagccagt cgggacctcc gccattttcg atgcccagca cgccgagtgg
      601 aatcttttgg aaatcgggtc gtgttgcttc cagaaatacg tctgccgcgc cagattccag
      661 aatgccgatc gcgccggcaa gagccacggc cccacccaac accaagctga caagcacccg
      721 tcgtttcatc ccgcggtctc ccctactcga aataccattt caacatcctg ataggcttcg
      781 ctcatctccg ccggaaaggg agggaacgcc cgcggtttca gaacagcccg ctggcctgcc
      841 atatcaaaat agctgttccc cgacgtctgc tgaatgacaa cgtccttaac ggttccattc
      901 ctatagaacc taaactttac caccgcgctg tacatttgac ccgtgacatc gatcggaggc
      961 ggctcccact ggctcttgat aatcgactcg atacgacccc aataggggtt ggttcccgat
     1021 gaaccggacg tcttcaattg ggtttggggc gccttcgttt gaggaatcgc cgcttcctgc
     1081 atcggtgccg gcttaaccgg aacatccttt ggaatctcta atttttctgc tggcttgaat
     1141 gctttgactt tcttgagttc ttccgccaat tcacgatcca attcgtcact gatcgacgtc
     1201 cgctgaggaa tctttacggc cggatcaggc gtcacgtccg gaactcgcgg caggtcggga
     1261 actttgacct ttgtatgagc ctgaggttgg ggttgagcca ctgatttcgc tggggtcagt
     1321 tccccgaact tcggcgcatc gggaggcaac tcgagatcct tgagaatgtc ctttccgaca
     1381 ctgctacgga caggcttggc cgcaggagtc acagccggtg tcacagactt gaccggtggg
     1441 gcaggagcag gtgctggtgc tggctctgct cgtttggccg tttcaacggg ctttgacact
     1501 tccacaagct tggccggcgc aggcaaactg accagagata cctcgaccga tgccaacggc
     1561 cgctctccgt gcggagcgag ccggagtccc gccaccacca gcaatatcgc acagtgcaga
     1621 accagcgaga tcgtgagggt ccggcccatg cgggcaaaga accgatcctg ctcctcctga
     1681 tccaaccaga gagccgactg cacatgtgcg ggcgccacca tacgtccgag cccctcattg
     1741 cctccgatat caactctttc gagaaggtgt tcgcggaatc gatggtcctc caacgcgctc
     1801 aggccccacc ggttccgtca ccattccgat cttctcgatt ccagccttct tcactccatc
     1861 catcacctgg accacgatgc catagggaac atcacgatca gcgcgcaggt acagggacac
     1921 gtcggcatgt tcgtgtttca ggatcttcaa tttttgttcg agctgtgcga cgccgatcgg
     1981 atccttgtct aagtagagcc gttgatcttt ttcgattgtc agcactgtcc gcatttcagg
     2041 cttgatcgtg tttgatgcag acttcggcag gttgatatcc atccccctat agagcatggg
     2101 ggccgtgacc ataaaaatca cgagcagcac gagcacgaca tcgacgagcg gaatgacatt
     2161 gatctccgcc atgaaccgcc gctgtcgcga ctcaaaggtc atcgctgcgc tccgacaggt
     2221 ataggtttgg ccttggccgg agatgcggac aacgacgcca tcaattcaat cgtgaccgta
     2281 tccaaccgga acgaggtgcg gcggatacgc gtgagaaaat agttataggc gatgaccgcc
     2341 ggaatggccg tgaataatcc ggccgcggtc gcgatgaggg cttccgccac tccgggagcc
     2401 acggccgaaa tactggccgt tccttgtgct ccgatctcgc gaaacgcatc gatgatcccc
     2461 atcaccgtcc ctagcaagcc tacaaaggga gtaatgttgc ccgtcgtcgc caagaagggg
     2521 agataggatt cgagtttgga cagctggcta tgagaaaggt gcgccgccgt gcgttcgatg
     2581 atgtgacgat cggccgatcc tgatccgccg ccgtcttgcg cttgtccgtg caaccccgcc
     2641 agacgatcca tcactccttc gaacagcacg gctgccggac tcgattccat gcggcgaacc
     2701 tggcggcaga gatcatccaa atccttcgac ttcgccaaca gcgccatgaa ccgctggtct
     2761 tcctgatctg cagcacgaaa tgcgcgccat ttggcaaaaa taattgccca cgacacgaca
     2821 gaaaagatga acagaagcag gaggacaatt ttcgagaccg caccgagcga gcccaacagt
     2881 ccggttaatc cgatctgaaa cattggctta catcctcttc cggctgcgtg ggcaagccac
     2941 acgcctacgt acggagtcta acaaatgctg gggaaaatgg cggggccgac gggatttgaa
     3001 cccgcgacct ccagattgac aatctggcgt cctaaaccag gctgaacgac ggcccctctt
     3061 gactctactt atagcggaag atctgattga caagactatt tgtagcggtt tgtcccgtct
     3121 tgaatttcaa ttttctgttt ctatccttga ttccggccac ccactcgtca cgcgaggttg
     3181 gtaggcggaa cagggatcga acctgtgacc tctgccttgt aagggcagcg ctcttccaac
     3241 tgagctatcc gcccgatttt tcaagcaagc tggccgatgc taccaagtcc acccggcctt
     3301 gtcaaccgaa tctcgcactc ttcttgcaga ttctttcaaa gtcttcattt tccttctctc
     3361 tatcacccca tcttttttcg cctgccttgg cgcgggaagt acgtggcatg aatttccttg
     3421 agccgttccc gttcgacgtg cgtataaatt tgagtggtcg cgatacttgc atgacccagc
     3481 atcgcctgaa cagaccgaag gtctgctccg ccttccaaca agtgggtcgc aaaggaatgt
     3541 ctcaggacat ggggagaaat tcgtttcgtg ataccggccc gtttcgctcg ttgacgaaga
     3601 ttcttccaaa acgcctgtct gctaagcggt ccgccgcggc gggtgacaaa gacgaaccgc
     3661 gacagccgct gcttcaaaac agacggccga atctgatcca catattgctg gagaaggcgg
     3721 cgggccggtt cgcccatagg gacgatgcgc tgtttcgaac ccttccccgt cacccgaaga
     3781 aatcctactt cgaaattcac ctgcgcagtc tgcaaggata ccaattccga aactcgcaaa
     3841 cccgtcgcat agagcaattc cagcatcacc cggtcccgtt gatcttccaa cccgggatgg
     3901 gacggcgcct ccagcagtgt agtgacttcg cgctgcgaca gcgtgttggg tagccgcatc
     3961 ccccgatgcc ctaccgtgac accgctggca ggattgtcgt cgatcatgcg ctcctgccga
     4021 aggaaccgga accatccacg aaccgtcgac agacagcgtg ccgaggacac ctgagagagt
     4081 tgcgcggtgt tcagtgagcc gagaaatcct cgcacggtgt ctgtggtcac ctgccgaatc
     4141 ggaatacggc tttgctgatg aagataggcc tgaaatttgg cgaggtcgcg tcgataggct
     4201 tcgacggtgt tgcgggagag gccgccttct actcgaagat ggttcaaata gcgctcgacg
     4261 agcggatccg tctccatcac gcgatgataa cgaatcactc ccatgaagac aacgcgcagg
     4321 cagagataac cttgacaccc taccgggatt ccgatactag tcactcttga cgagtgagtt
     4381 cgtgacacca tcgacacaga catggccagc cggttcacac cgcgcctatg taccagtccc
     4441 atgccattga agctcgcaac agcccgcctc accttcacac tgacattttt ttgtgtcggc
     4501 ctcggctcac cgtggcccgt cttccatcct accgtcgcag aagcggctaa aaacacgccg
     4561 gccaatcacc cggttctcaa ccaggccaag cgattgatcg acgcaggcca ggcggaagaa
     4621 gccatcacga tgctccgcca attcctcaat acgaacccca agcaagatct tctcgacgac
     4681 acctatctgc tcctaggggc ggccctgtat gccgcccaac aatatggaga gggcctcaag
     4741 ttcttacagc agcttcaagg agagttcccc aactcagaag tcatcgaccg cgggaagctg
     4801 atgctggctc gcacacacgc ggccatggga aatccggatc ttgctctccc catcctggcc
     4861 aatcttcgca cgctgtccca agacgacatc accaaacggg aagccgtgca tctgagcggt
     4921 gaactgtatt tccaaaagaa agaaccgacg cgcgccattc aggcctggct cgatgagatc
     4981 gcgctcagcc cggaggacca aaccgacgaa atccgtgccc atatccgcga ggtgatcaac
     5041 ggatccttcg acaagaaaat gctcgatcgt atcagggagg cctatccaaa gagttttccg
     5101 ggtgatcttg cctcgcttcg cttgatcgat ctctatgtgg gccgcggaga agaccaccaa
     5161 gccgccaggc agattcaacg attcctcgca aatttccccg cgcatccgca ggcggccaag
     5221 tcgtccgacc tactcgcatc gttacaaacc aaactcaaat cttaccaatt tgtgatcgcc
     5281 gcggtacttc cgttgtcggg gaaactcgcc cccttcgcca acgacgttct cagcggagtc
     5341 cagctggctg tcgaactgaa tggtgatcga gcgggaggcc catctgtcgg tctcctcgtg
     5401 agagacttcg aatccaccca agcctcgttc ttggacgact tcggtggcct gctcaactac
     5461 gatcgcccga ttgccgtcat cggcccgcta ctgtcgaaaa atttgcccgt gatggcagaa
     5521 ctggccgaac gttcacgcat tccgctccta acccctacag ccaccctgcc gaatgtccga
     5581 cgactgggtg cgtttacgtt tagtacggcc ttgaccttcc agctacaggc caaacggatc
     5641 gccgcctttg cgacaggcga acagggatac cgacggttct gcatcctgca tcccgataca
     5701 gcctatgggc gtgaactggc gcgcttattc gccgaggaag tgcgcctcca ccaaggcgaa
     5761 atcattgcga tcgaatccta taaagaaggc gagtccgatg tcggcaatca gctcaaacgg
     5821 atgaaggctg aagatctcaa aaaatacggc ctcgcggtgc cggtcgatcc gacgaaaacc
     5881 ggtgggaaac tgacgaagct cgataagaaa atcctctaca cgccgggctt cgacgcgatc
     5941 ttcatcccga gccgcgcctc agatgtcggg ttaatcaccg cccaactgaa tttccacgac
     6001 atgaaagtgc cgatcctggg cagcaacggt tggaatgccc cggacttcgc ccgcacagcc
     6061 gatcaatcaa ttgacggcgc cgtgttcgtc gacgggtttt ttgcggacag cccgaatccc
     6121 aacgtgcagg atttcgttga gcgatacaag aaacgctttc aggcgacgcc gacgctcttt
     6181 gccatgcagg ggtacgatgc ggtgaagttc gtcatcgaag cggtgaggaa aggcgcaacc
     6241 tccggtgagg ccgtgcggga ctatctcatg tcgcaacaag atcttcctgc actgggagga
     6301 ccggcttcgt tcggacagga cggcacgctc aatcgaccgc tgtttctcat ccaagtcaaa
     6361 cgagggcgct tcacacaagt cgactagcgc tacaccggca tgaacgatct accgcaactg
     6421 cttttttcca tttcctacaa agcgcttccg ctcctcttcg ccatggtgct gcacgaatac
     6481 gcccacggat gggtcgccaa caaatgcggt gatcccaccg cgaagctgca gggacgactc
     6541 acgatgaacc ccttggccca tatcgatctg ttcggcacca tcatcatgcc cctcctctgt
     6601 ttgatgctgc cggggagctt tctcctcggc tgggccaaac cggttcccgt cgatgccaga
     6661 aacatgaggc agccacgccg cgacatggcc ttggtggctg cggcagggcc agggatgaat
     6721 cttgcgctgg ctttggtgag cgcgctcctg ctggccgtct tgttggcaat tgacccctcg
     6781 ctctcgatag acaacagctc cgccgacaaa acggagtcag gcgccaccct tttcctcttg
     6841 ccgatcgcgg tcatggcgct ctactccgtc ctggtcaatg tgttcttggc aatattcaac
     6901 ttgattccca tcccgccgct cgacggagga cgcattctcg tgagcctgtt gccgccgcga
     6961 ccggcgatgg ccctcgtgcg gcttgaaccg tacggcatgt tgattctggt cggcctcctc
     7021 gtattcgacc gcgatcttcg cgtgatccat acgattacca ataccttcgc ttcgggcctt
     7081 tcgggtacaa tcctttccac cgcgatcggc ctcggtgcag gagcgtccca atgacgatgt
     7141 cacggaaacg cgtgttgagc ggcatgcagc ccagcgggct catgcacctc ggcaattatc
     7201 tcggcgcctt ggaaaactgg aaagcgctgc aggatcaata cgagtgctat ttttttgtcg
     7261 cggactggca tgccctctcg accaattatg ccgataccag ccgcatccgt gagtatgtgc
     7321 gcgagttgct catcgactgg ctcgccgccg ggatcgaccc cgcccgcgcg accgttttca
     7381 tccagtcgcg cattccggaa catgcgatct tgcatttgct cctttcgatg atgatcccgg
     7441 tgtcctggct ggagcggaat ccgacctata aggaaaagca ggaagagatc aaagaaaagg
     7501 acctgagcac ttacgggttt ctcggctatc ccgtgcttca agctgccgac attctgctgt
     7561 acaagcccga tttcgtcccg gtcggaaagg accagctccc ccatctcgaa ctcacgcgcg
     7621 aagtggcaag gcggtttaac gacctttaca agaaacccgt gtttcccgag ccaaaagagc
     7681 acctgacaaa gtttccgaaa gtcctcggca ctgatggccg taagatgagt aagagttacg
     7741 gaaacacgat caatctctcc gat
//
LOCUS       DATIFR010000091         6307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_53853, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000091 DATIFR010000000
VERSION     DATIFR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53853"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..287)
                     /locus_tag="VLD60_05210"
     CDS             complement(18..287)
                     /locus_tag="VLD60_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44388.1"
                     /translation="MESEDNVLDELLSEIAGLINAYPKAIERRAAMIQATGKDPELAE
                     KLVKAADTMRDSGNLYLTWAKHYAAMAAGNTDASSDEDETEDFDV"
     gene            complement(361..639)
                     /locus_tag="VLD60_05215"
     CDS             complement(361..639)
                     /locus_tag="VLD60_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HSB44389.1"
                     /translation="MYNVAQVIDEKCTAKKGCRLCIMYCPEANCLDLNPTKMVAEVNI
                     DRCKGCELCVVVCNAAKHMAIEMQAVSATGQLMHHKGESAALGQAYQG"
     gene            complement(663..1370)
                     /locus_tag="VLD60_05220"
     CDS             complement(663..1370)
                     /locus_tag="VLD60_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSB44390.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVLAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIEVFHPQVITMGKSYTMPFYSGVKEGGVV
                     IINSDQSLLSEEDIQRLKDLNVAVFYIAGTELAIEVAGTELSTNMAMIGSVAGITKCV
                     SMEALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIASEW
                     AEKNNIELRVGNPAVAA"
     gene            complement(1389..2291)
                     /locus_tag="VLD60_05225"
     CDS             complement(1389..2291)
                     /locus_tag="VLD60_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSB44391.1"
                     /translation="MSKEKIKISEDLYDIMPSDYQDLVKSATYGKEDRGWKDIGNSKE
                     LIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTGCTSLVFPMVAVHNIHSLFGNQ
                     NAIASGLKRALSVRFPDRTKDVVVLAGDGATVDIGLDMTLQAWFRQEKFTTICFDNEL
                     YANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIARESGCHYVVQCTVSKPSLIEKV
                     IRNAVFIAREIGPTYLQLYTPCILEIGKNSMEGLQEMRDSEKPTERFAYKEYVSEPAK
                     QLLAEIAAKDKERKTAAKQLAGQA"
     gene            complement(2347..3558)
                     /locus_tag="VLD60_05230"
     CDS             complement(2347..3558)
                     /locus_tag="VLD60_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSB44392.1"
                     /translation="MADTHSVIGTKNKKGQTYTDTWKMMNEAPRTPSFYTGSEVIKEA
                     VRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGML
                     VWHAETAQDFFDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEIWAAVERSRKHSIKWL
                     NGLIDTENTDAEIMIVASGTAVSQGREAIRLLEDEGIRCGLVKVKTLRPWPEEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIKATIPNHHRVHAGPHVCGGMTMPPEIIVQEIKTALG
                     MKTFSLAGRGS"
     gene            complement(3600..4235)
                     /locus_tag="VLD60_05235"
     CDS             complement(3600..4235)
                     /locus_tag="VLD60_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HSB44393.1"
                     /translation="MSQYRVLPGPEHFLPPAAASMGICLPNPGEAHINGVIVPEEKAY
                     EEAAKQFLMAKVPTIFPGPLVLWAWNEKAAKKATAIRHLYNTLKECVQPGQTPMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFVGVHCHQANLSLKIIRGGTSCYT
                     IAMCAQAGHEDAMLSFRDASVEKIMKLADWVKKLKGTVQPRLTSAKSGASN"
     gene            complement(4595..5863)
                     /locus_tag="VLD60_05240"
     CDS             complement(4595..5863)
                     /locus_tag="VLD60_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44394.1"
                     /translation="MRFQGWTFFAAGLSLLLLSNPSVVAAAGKIVVAEAQYVMADGDT
                     LASAEEKVLQRAQRRAVEEAGVYLESTFHDFEKESQGQSTRATSLEIRTIAAAITETE
                     ILESHRSFENDRPVFFVRIRATVDLHNLAEAVRRLQSEAQLAQHFRQLQNENQHLRAQ
                     LKELQQQPTGVRMLAIDPSGKSMPAQRAKSLLEAALHTQDLREKIQLASEASQLDQDS
                     AEPLIVRGQTYLRLVSVAYSQRGNPDDYAAQVEKAKGDFDRAVQLDGKNPWGWVGKGD
                     AQTWLKHMDDAAISYEQALALDPFFDIARQRLIGVYTTQARRQAEGKHWHQALATLKR
                     LLDAQTPESWIPYQKEALLLRSEILLKLNRQEEALGDLSTVIRVDPTNANALVTRANI
                     YRNRLQGRMAKDDLERACVLGSIPACEQLP"
     gene            complement(5999..6277)
                     /locus_tag="VLD60_05245"
     CDS             complement(5999..6277)
                     /locus_tag="VLD60_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB44395.1"
                     /translation="MKFVILGFDGPDGEAKRKIYRPAHLAKMDPLDQQGRVVLAGPLT
                     DKTGSLIVIEADSLEEAQRFAQEDPYTVNGVFERVEVHPFTQVFPKPR"
BASE COUNT         1239 a         1753 c         1761 g         1554 t
ORIGIN      
        1 ttttttcacg ctccgtgcta cacgtcgaag tcttcagttt cgtcttcgtc cgaggaggcg
       61 tcggtgttgc ctgccgccat tgccgcgtag tgcttggccc aggtcaaata gagattgccg
      121 ctatcgcgca tcgtgtccgc cgctttcacg agtttctcgg ccagttccgg atctttccct
      181 gtggcttgaa tcatggcggc ccgccgttca atcgctttgg ggtacgcatt gatgagtcct
      241 gcgatctcac tgaggagttc atcgagcaca ttgtcttcgc tttccatggg ttacctctta
      301 tctcaacaaa aaaccccata gcgcggacgc catggggttt tttgctcgaa ctgtttcagc
      361 ttagccctgg taggcttgcc ccaaggccgc ggactcgccc ttgtgatgca tcagctgtcc
      421 ggttgcactg acggcctgca tttcaatcgc catgtgtttg gccgcgttgc acacgaccac
      481 gcagagctcg cagcccttgc agcgatcgat gttgacctcg gccaccatct tggtcggatt
      541 gaggtctagg cagtttgctt ccgggcagta cataatgcac agtcggcaac ctttcttggc
      601 cgtgcatttc tcgtcgatga cttgcgcaac gttatacatg cgacgtggtt ccttctctct
      661 cgttaagccg caacggcagg attgccgact cgcagttcaa tgttgttctt ctcggcccat
      721 tcgctggcga tctcataggc tctcttgacc gtcgccagat tcttcgccaa caacatttct
      781 ttcttggcga atttcttctt aatggcttcg tccagggatg ccgtaccgcc tgaagccacg
      841 aatttctttc caaagcgctc ttggagcgcg ccgtcgagcg cctccatgga gacgcatttg
      901 gtgatgcccg ccacggatcc gatcatcgcc atattggtcg ataactcagt gccggcaacc
      961 tcgattgcga gctcggttcc cgcaatgtaa aacaccgcga cgttcaaatc tttgagacgt
     1021 tgaatgtctt cttccgagag cagagattga tcggagttga tgatgacgac gcccccttcc
     1081 ttcacgccgg aatagaaggg catcgtgtaa ctcttgccca tggtgatcac ctgaggatga
     1141 aacacctcga tcacgtccgg gaacaccaac tcgccacggt cgtatattcg ctcgattccg
     1201 atacggcagt agctctctgc cggcgccatg cgcttctcgg caccgaaaaa cggattagaa
     1261 atcgaaaact tcccgtcccg gttggcagcc atggccaaga catgcgccgc agtgacggcg
     1321 ccttgcccgc ccaggcccga catccggatg ttgagtctct tcttaatcat gaatgacctc
     1381 cggcttcttc aggcttgtcc tgccaattgc ttggcggcgg ttttccgttc cttgtccttc
     1441 gcggcgatct cagccaagag ctgcttggct ggctcgctga catattcctt gtacgcgaac
     1501 cgctcggtcg gcttctccga gtcacgcatt tcctgcagcc cttccatgct gttctttccg
     1561 atttcgagga tgcagggcgt gtaaagttgg agataggtcg ggccgatttc tcgggcgatg
     1621 aagaccgcat tgcggatcac cttctcaatg agagacggct tgctaaccgt gcactggact
     1681 acgtagtgac aaccagactc acgagcgatt tccggcaacc ggaccttgtc gaagagtttc
     1741 ccaactggcg ccatttttgc cacgaagccc ttctgcatga gcccgctctc ctggccgccg
     1801 gtgttggcgt agagctcgtt atcgaagcag atggtcgtga atttttcctg gcgaaaccaa
     1861 gcctggagtg tcatgtcgag accgatatcc acggtcgcgc cgtctccggc caacacgacg
     1921 acatcttttg tccggtcggg gaaacggacg ctcaaggcgc gcttcaatcc ggacgcgatg
     1981 gcattctggt tgccgaacag ggagtgaatg ttatgaacgg ccaccatcgg gaacaccaag
     2041 ctggtacagc cggtggagcc caccatgact gtatcttccg ggttcgggag cgaagctagg
     2101 atgtagcgga acgccataga ctccgggcag cccgcgcaga gcgagtgttg ctcgataagt
     2161 tctttggaat taccgatgtc tttccagcca cgatcctctt tgccgtaggt cgcgctcttg
     2221 acgaggtcct gatagtccga cggcatgatg tcgtagaggt cttctgaaat cttgattttc
     2281 tctttgctca tgtattcctc ctcagattcg caagtgcatc gcgaactggt tctggatgtt
     2341 tactgctcag cttccacgac cggccaggga gaaggtcttc atcccgaggg ccgtcttgat
     2401 ttcctgcacg atgatctccg gcggcatggt catacctccg cacacatgcg gtccggcatg
     2461 gacccgatga tgattgggaa tggttgcctt gatctctttc gccagccagc cggtcacgtt
     2521 gaactccggt acgaagatgt gcttcgcgtt cttggtggct tcgcggattt cttcctccgg
     2581 ccacggtcgg agcgtcttta ctttcacgag tccgcagcga atgccttcgt cttccagcag
     2641 gcggatcgct tcgcgtccct gtgacacggc cgtgcccgac gccacgatca tgatctctgc
     2701 atcggtgttc tcggtatcga tcaagccgtt cagccacttg atcgagtgtt tgcgggatcg
     2761 ctcgacggcc gcccagattt cctgctgcca gcttgcgtgg gtggcataac tgatgtagtt
     2821 gctcttcatg atgaatggat cgcgcatcat tcggaccggt ggacattcca tatccatgca
     2881 gggaaccggc gagcgatagg gatcatatgg cggcaggcac atgtcggccg gcgtcagatt
     2941 cacgacgtcc ttggtgtgcg tcacaaagaa gccgtcgcag cagagcgcga gcggcaggtg
     3001 cacgtccggt tcttccgaaa ccatgtagcc cttgagaatc caatcgaaga agtcctgcgc
     3061 ggtctccgca tgccaaacaa gcatgccggt gttcaacaga taggcgattt cgagcgtgtc
     3121 aggctgaatc gacagcggcg aattgatgcc gcggcaggtg acgatcattt ggatcggcaa
     3181 acgcgcaccg gcccacatgg ggaagttttc catggcgcgc atcgtgcccg gtcccgctgt
     3241 ggtcgtgaag acgcgggcgc caccgaatgc ggcgccggcg cattgcgaca tgacggcgaa
     3301 ttcgctctcg ccgcggaagt agtcgccgat atagccttcg gcgaagagtt caccgatgag
     3361 tgccgcggct tcgctctggg gagtaatcgg gtaggcgatc atgacgtcac aactggctcg
     3421 ccggaccgct tccttgatga cttcgctgcc cgtatagaac gacggcgtac gtggcgcttc
     3481 gttcatcatc ttccaggtgt cggtatacgt ttggcccttc ttattttttg tgccgatgac
     3541 tgagtgtgta tccgccatgg acccagcctc ctttcactgc gtacggtgct tcaaacggtt
     3601 tagttcgaag ctccgctttt ggctgacgtc agccgtggtt ggactgtccc tttcaacttt
     3661 ttcacccagt cagccagctt catgatcttt tcgacagacg cgtcgcggaa agacagcatg
     3721 gcgtcttcat gcccggcctg ggcgcacatc gcaatcgtat agcacgaggt tccgcctctg
     3781 ataatcttga gcgagaggtt cgcctggtga caatgcaccc caacgaacat gcagcaatcg
     3841 atcttattgt gccagatggt caggttggga tggtttggat taatttcgac ttcgggattg
     3901 atcttcggat acttggggcg atagtccggc atgggaatca gcatgggcgt ctgacctggc
     3961 tgcacgcact ctttcagcgt gttgtagagg tgccggatgg ccgtggcttt cttcgcggct
     4021 ttctcattcc aggcccacag gaccaaaggg cctgggaaga tcgtcggcac cttggccatc
     4081 aagaactgct tggcggcttc ttcgtacgcc ttttcttcgg ggacgatcac gccgttgata
     4141 tgtgcttcac ccgggttggg caagcaaata cccatggagg ccgctgcagg aggaaggaaa
     4201 tgctcaggtc ctggtaacac acgatattga ctcattacga cctacactcc gcgtaagggg
     4261 ttaacgacgt gagaacttag atttttcaac cgaatccatc tatcaacaat ctatgctttt
     4321 cacggggaat ctgcaacctc gcagaaggcc caacttctgg aaataatggg ctatcgggca
     4381 agcgagaatt aggccttttg tcctaatgtg ctggagaaat tgcccactca aaacagcaca
     4441 atcttgcgac aggtggggca ttataggagg gccatttccg tgaagtcaaa cagacttccg
     4501 taggggagtt ttgcgatgga agagtgacga tgaaggcgga taatggcttg aaatttaatg
     4561 gcttaggggg gttgccggcg tggaaattgc tcatctaggg cagttgttcg cacgcgggga
     4621 tagagccaag cacgcaggct cgctcaagat cgtccttggc catccggcct tgaagccgat
     4681 tgcgataaat attcgcgcga gtcaccagcg catttgcatt cgtcggatcg acgcgaatca
     4741 ccgtggatag gtccccgagc gcttcttcct gccggttcag ttttagcaga atctcgcttc
     4801 gaaggagtaa cgcctctttt tgataaggaa tccagctttc gggagtctgt gcgtcgagca
     4861 gcctctttaa ggtggcgaga gcctggtgcc agtgttttcc ttccgcttgt cgccgtgcct
     4921 gggttgtata gactccgatg agacgctgtc tggcgatatc gaaaaacgga tcgagagcca
     4981 gggcttgttc atacgagatt gctgcgtcat ccatgtgctt caaccatgtc tgcgcatcgc
     5041 cttttccaac ccagccccaa ggattcttgc catcgagttg gacggcacga tcgaagtcac
     5101 ctttggcctt ctccacctgc gccgcgtagt catccggatt cccacgctgt gaataggcca
     5161 ctgagaccaa ccgcaaatag gtttgtcctc tcacgattaa cggctctgca gagtcctgat
     5221 ccaattgaga ggcttcagag gccagctgaa tcttctctcg tagatcttga gtatggagcg
     5281 cagcttcaag cagactcttg gctcgctggg ccggcatgga ttttccgctc gggtcgatgg
     5341 caagcattcg gactcccgtc ggctgttgct ggagttcttt gagttgggcg cgcaggtgct
     5401 gattttcatt ctggagctgg cgaaaatgtt gagccagttg cgcctcggat tgcagccgac
     5461 gcacggcttc ggcgagattg tgcaagtcga cggtggcccg tatccgcacg aaaaatacgg
     5521 ggcggtcatt ctcgaatgag cggtgcgatt cgaggatttc ggtttcggtg atcgccgcgg
     5581 cgatcgtccg aatttccagc gacgttgcgc gggtgctttg tccttgcgat tctttctcga
     5641 aatcgtgaaa cgtcgattcc agatagacgc cggcctcttc caccgctctg cgttgggcgc
     5701 gctgtagcac cttttcttcc gcgcttgcca acgtgtcccc atcggccatt acatactgag
     5761 cctcggccac aacaatcttc ccggcggcag caacgactga aggatttgaa agcaggagga
     5821 gagaaaggcc agcggcgaag aacgtccaac cttggaatct catggaatta actataccgc
     5881 ccgtccgggg cctcagcaag gcaacgaaag gtgggaatcg cttctcgcgc gatgtgagga
     5941 gttgagggaa ttgtccgtgt aaacggggag ttgcgctcgc cctggattgg gggccaatct
     6001 atcgcggctt gggaaagacc tgggtgaagg gatgaacttc gactcgctca aacacaccat
     6061 tgacggtata ggggtcttcc tgggcaaaac gctgagcctc ctccaatgag tctgcttcaa
     6121 tgacaatcaa gctcccagtc ttatcggtaa gcggaccagc cagcaccacc cggccttgct
     6181 ggtcgagtgg atccatcttg gctagatggg ctgggcgata gatttttcgt ttggcttcgc
     6241 catccggacc gtcaaaaccg agaatgacaa atttcatggc tgaaaatgtg gctttcctgg
     6301 gttactg
//
LOCUS       DATIFR010000092        12462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2353547, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000092 DATIFR010000000
VERSION     DATIFR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353547"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..422)
                     /locus_tag="VLD60_05250"
     CDS             complement(195..422)
                     /locus_tag="VLD60_05250"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB44396.1"
                     /translation="MKLSTKPQKFHATPSGHGAKTARVGASRQSETDSFDGVYSRIAF
                     LAYQFYEQRGRVDGYDMEDWIRAEQTILAAK"
     gene            complement(441..1463)
                     /locus_tag="VLD60_05255"
     CDS             complement(441..1463)
                     /locus_tag="VLD60_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSB44397.1"
                     /translation="MDQEGGDGMAGRVKDLSRRWSIVMAGSEAECVRPFIQRWLGHPR
                     PKQYCTFVGTRSLFQHTLDRAARLSRPEHIVTVIPREHRPEAWSQLEGRAVGTVLFHP
                     KNRNTAAGIFLSLTYIRARDPQAIVVIYPSDHFVYPEERFLELVQRAAWTAEWLPDRL
                     VFLGGSPDGLELEYGWIIPGKKLDGSPKYQIRAVDSFLPQPTAAQADAALTRGALWNS
                     SVVVAKAALLWELGWKCFPDIMPHFERLSHSIGTPQEVRILDEVYRDMPAHHFSSELL
                     QHVPDQAAVIEMSGVLWSNWGKPERIVHTLRQIGRQPAFPLTCLNRPFAPISQVGSDN
                     GVLAEI"
     gene            complement(2295..2597)
                     /locus_tag="VLD60_05260"
     CDS             complement(2295..2597)
                     /locus_tag="VLD60_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44398.1"
                     /translation="MASTGEVEKDILQDLERRGPCSFEEIVTHLPAYTWNQVFSAVDR
                     LSRNAQVTLQHPSRFGYLIALAQAYRPSAPVTGTTGGHRSAGRVDRAHATVRGTAS"
     gene            complement(2825..3457)
                     /locus_tag="VLD60_05265"
     CDS             complement(2825..3457)
                     /locus_tag="VLD60_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB44399.1"
                     /translation="MLRILVVDDHPFFRRGVKDILVEGFGTVKFGEGGNAHEMLELAR
                     QKKWDIVVMDISMPGRSGPDALKQLKAEHPKLPVLVMSMHPEDQYAIRMFKAGADGYL
                     TKASAPEELVRAVKKVLEGGQYVSPSLGEKLALRMKVGEAGAPHEQLSDREYEVLCLI
                     ASGKTVSEIAGVVNLSVTTISTYRTRILEKMNMKNNAELTRYALQRGLVS"
     gene            complement(3476..6475)
                     /locus_tag="VLD60_05270"
     CDS             complement(3476..6475)
                     /locus_tag="VLD60_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB44400.1"
                     /translation="MRILLVEDNPGDIRLLQEYLVKDSSTTRGFQIVHADRLEKGLTH
                     VAEAGFDAVLLDLSLPDSHGMDTLIRMRAAAKGIPIVVLTSIEDDALGMRLIQAGAQD
                     YLVKGQVTGHLLMRSLRYAAERNRTESALRDSEERYRTLVEGARDVIFTLSKEGVITS
                     LNPAFERITQWTSDAWVGRPFGELLHPEDLPLAIDIFRHIVETGEALTCVFRIRSQDR
                     GYVVGEFVGVPHIQNGRPCGVLGIARDITERKRVEEALERLRHRYVLLLTSAHDGIYG
                     IDMQGHAIFVNPAAARMIGSSSEELVGRCMHDIVHHTKQDGGTYSVEECPIHRTIRDG
                     TVQYMADDVFWRKDGTSFPVEYSSTPIIERGEMVGAVVMFRDISERQRTERALRESEA
                     RLRAILDNSPNLVFLKDTQGRYLDVNRQFERTFHMSREAIAGKTDEEVFPSEQAAAFR
                     ANDLMVLRAGVPLQFEEVALQDDGPHTSIVFKFPLHNGNGKLYAICGITADITERKRA
                     DEERRKLLNDRLLLLESTSDGIYGLDLDGRCTFINTAAAKMLGYEQSEVVGKNMHDLI
                     HHHRADGTPYPVNECQIFQVFRSGRASHVDGEVFWRKDLSHFPVDYSSSAIVEEGIIK
                     GAVVSFTDISQHKQAEEAIRQAEERYRSIFENAVEGIFRTTIDGKYLVVNPALARLYG
                     YESPEEMISAVTDIAKQVYVEPRRRDEFLRRMHEEEMVIGFESQARRKDGGVIWVSEN
                     VRAVRDRNGAIVGYEGTVENISQRKEAEEQLQQSLDRLRTLSGRLEVVREEERARIAR
                     ELHDELGVGLTCLKIDLSQANTIVSEVEGSRLRRKLSEKIRAMIEQVEGTITDVQRIV
                     SELRPGVLDDLGLVAAIEWQSQEFQRLTGITCTCTATAEDIEVEPERATAVFRICQEA
                     LTNVARHAHATAVRVSLQEQGGSLLLSVSDDGKGIPDQKISDPRSFGLLGMRERAALL
                     GGEVTITGSPEKGTTITMRLPVRTPAA"
     gene            complement(6485..6949)
                     /locus_tag="VLD60_05275"
     CDS             complement(6485..6949)
                     /locus_tag="VLD60_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44401.1"
                     /translation="MSVSELVKPIEILLIEDNPGDVRLTMEALKETKVINKLTVMKNG
                     LEAMAYLRREGPHANAARPHLILLDLNLPIKDGREVLAEIKADESLRRIPVVVLTTSK
                     DEQDVLKSYHLHANCYITKPVDLDQFITVVKSIEDFWLGIVVLPKNGKHRSV"
     gene            complement(7024..9321)
                     /locus_tag="VLD60_05280"
     CDS             complement(7024..9321)
                     /locus_tag="VLD60_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44402.1"
                     /translation="MNPTRYGRFLPAVLLLCFAIAVGLGLHALGVLRAVLVSERGADL
                     ARAAAQAADTLDRVLFERFGDIQVFANDRILLQGSRDEKEKRLQEYRRLYWYYSWIGV
                     ADASGRLVAATDLSVSRSKNAESDESSGSGVSADQRDWFEIVRQTGKIHLAEARPSGE
                     SGGVMAVGFSAPILGPHGEFQGAVATQVPLENLRAILQQEGALRYREEAFDWLLLDSR
                     GVIISEKEQANGMTGGALKEALPSVVRAAADRSQSGFVEELHQRRQVPVVTGYARTRG
                     YNKFPGFDWTLLVRLDRDRAYAPINRLIWTVGGVGLLLVAPLTGFGIWAARRVAQEQR
                     DLLVAQRDLAESIQISEERARALHSLVGAVREVTASPELDRLLEQILHGAREVTGARY
                     AALGIVDEAQGKLARFITSGMDEATKTAIGALPTGRGILGLLSHQEGALRLKNLMEHQ
                     AAIGFPSHHPAMRSFLGVSIRTHERVFGRIYLTEKQGADEFTQLDEEMIIALASHAGK
                     AIDQTLLLQQVQRAENQLRQVNEELIRSNADLQQFAYVASHDLQEPLRMVASYTQLLA
                     KRYKGKLDADADEFIAYAVDGATRMQRLINDLLAYSRVTSQAKAFELVDCNQLLEGVL
                     STLRLAIEESRAVVTRDALPKVMADSGQLAQLFQNLVSNAIKFHGAEPPSVHVAAERR
                     NDEWLFSVRDNGIGVDPQFADRIFVIFQRLHDREEYPGTGIGLALCKKIVERHGGNIW
                     VESQPGRGATFFFTIPIEQRRDSMS"
     gene            complement(9776..10225)
                     /locus_tag="VLD60_05285"
     CDS             complement(9776..10225)
                     /locus_tag="VLD60_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSB44403.1"
                     /translation="MWNRIVPAACLVAIVMVVPSVQAEGFSDKGGADRSHRVVMHLNS
                     GDGKMQKGTLNNIRHLYQELGREQLQLELVVHGAGLSLFTKNATALGQELAILKADYG
                     VHYTACSNTMKAMKITRDDLIGEVEDTVPAMVRLMERQEQGWVYIKP"
     gene            complement(10246..10797)
                     /locus_tag="VLD60_05290"
     CDS             complement(10246..10797)
                     /locus_tag="VLD60_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44404.1"
                     /translation="MSDREDGAERNLEQSYDHLLTLWTSGVRDYHSMLSDYLTANSIF
                     VAVIGLTVSRESPALSLTVLILLLCVFGILLCLQMAIVLGRFSGQNALWEWQLRGIEQ
                     AQEWNKHKLITGLYRLHETQQPVEDLQNDPPVFKPNWAFRQHRQWWAHRAVSFPWFFG
                     CVYGVFLLWALTQLSRQLSGGSS"
     gene            complement(10815..>12462)
                     /gene="glgP"
                     /locus_tag="VLD60_05295"
     CDS             complement(10815..>12462)
                     /gene="glgP"
                     /locus_tag="VLD60_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSB44405.1"
                     /translation="GLGVLAGDTIRSAADLDVPMVAVTLLHRRGYFYQRLDAQGWQTE
                     EPVAWPINDFLKPVDQRVTVEIEDRIVQVRAWSCRVTGESGGEVPVYFLDTDLSDNQP
                     WDRTLTDVLYGGDDHYRLCQEMVLGFGGYRMLKALGYGDVRRFHLNEGHAALLVLALL
                     EDKLASRAQGDAVPSDLIDAIRERCVFTTHTPVAAGHDQFPPDLAHRVLGPRRCGWLQ
                     ACGQDSRLNMTQLALRGSRYVNGVAMKHGEVSHNLFPGYPIHSITNGVHAATWAAQSF
                     QQLYDRHLPDWRRDQLSLRYAVSIPASEIWDAHLEAKRALIEYANRETNTGFEHDVFT
                     IGFARRATAYKRVDLIVRDIERLKAIAQRVGRLQLVFAGKAHPADQDGKQVIHHIHEI
                     REALRGVIAVSYLGNYDMSLAKLMCAGVDVWLNTPLPPMEASGTSGMKAAVNGVPSLS
                     VLDGWWIEGHVEDVTGWSIGDRVDSSKPRVDVDAEHAVDLYRKLEEKVIPCFYQDHER
                     FMEIMRHAIAVNAGFFNSQRMVTQYLHNAYRLAGDYLRHG"
BASE COUNT         2628 a         3824 c         3271 g         2739 t
ORIGIN      
        1 gatctgcaaa ttctgccaaa gcccctgggc ggcctcactc ctcgtgttat ctcggacttt
       61 gtcacagaag caacagaccg acagcatcga tttcatctta cctaacccgt gcatgttctt
      121 gcacggtcct ccttgtgtca gaggcccacc aaagaaccaa acctgttgtc tttcgtcatc
      181 gccacgagcc actatcactt ggcggcaaga attgtttgct ctgcccgaat ccagtcttcc
      241 atatcataac cgtccacgcg accccgctgt tcgtagaact ggtaggccag gaaagcgata
      301 cgactataaa cgccatcgaa tgagtccgtc tcggactggc gcgaggctcc gactcttgct
      361 gtcttcgcac cgtgcccgga aggggtcgca tggaacttct gcggtttggt actgagtttc
      421 ataaccgatt tctccttttg ctatatctcg gccagcacac cgttatcgga accgacctgt
      481 gagatcggcg caaagggccg attcaggcag gtcagcggga aagccggttg acggccgatc
      541 tggcgaagag tgtggacgat ccgttctggc ttaccccaat tactccagag cactccgctc
      601 atctcgatga ctgcagcttg gtcaggcaca tgttgaagca actcggacga gaagtgatga
      661 gccggcatgt cccgataaac ttcatcaagg atccgcacct cctggggagt tccgattgag
      721 tgactgagac gttcgaaatg cggcatgatg tccggaaaac acttccatcc gagctcccac
      781 aataatgcgg ccttggctac cactacagac gagttccaca aagctcctct ggtcaacgcc
      841 gcgtcagcct gagctgcggt cggttgcggc aggaacgagt cgacagcccg gatctgatac
      901 ttgggggatc catcgagctt tttacccggt ataatccaac cgtattccaa ttcgagacca
      961 tccggggaac caccgaggaa gaccaggcgg tctgggagcc attcggcagt ccaggcagca
     1021 cgctggacca attcgagaaa tcgctcctca ggatacacaa aatgatctga tggatagatc
     1081 acgacgattg cctgaggatc gcgggctcta atgtacgtca gagataagaa gattcccgcg
     1141 gctgtgttcc ggttcttcgg atgaaagagc acggtaccga ccgcccgccc ttcaagctga
     1201 gaccatgctt ctggtctgtg ttcacgcgga atcaccgtga caatgtgctc cgggcggctg
     1261 agtctggccg ctcgatctaa cgtatgctgg aacagcgacc gcgtgccgac aaaggtgcaa
     1321 tactgctttg gtcttgggtg acctaaccat cgttgaatga acggccggac gcattccgcc
     1381 tcacttccgg ccatgacaat ggaccaccgc cggctcaagt ccttcacgcg cccagccatc
     1441 ccatcgcctc cttcttgatc catcgcgaat agcctcctct agcaggatgt tgaaaaagtc
     1501 caccagctgc gttctcgcct cgctcagaga ctcaacgtac ggaacagagt acgattcgcc
     1561 tcttggcttg ctgcggcctt gccggacggc ctttttgacc atcctgcgag ctgttctggt
     1621 tctgtcagga acattcccat caatatttcc cacaggggtc gataatagtt tttcaacagc
     1681 ctcctagggt tcgccacacc tcgatgtcct cgtcagagcc tgctggttac ctgtggcgcc
     1741 cagacgctac aagcgaccac tccataaatc acccgttgcc aagcacggat gagactggct
     1801 gtccggattc tgctcgaagg ggaatcgctc gcgggtcatc cttcaggcaa tcatgacagt
     1861 acgtgtggga gaagttgatg tcgtcaggcc tcaccttgtg cgccaccatg taaattctca
     1921 actcctgcca cagccctcgg ccggcctcag tcccggtgtc atctcgaact ttctggcaga
     1981 agcagcaaac gggcagtatt tgtttcatgt gttcctccat ccgacagttc atcatttgaa
     2041 tctccccatg accggcctgc gtctcatcga cggacgatgc gggctgaccg caaggatcga
     2101 cactctgcga ctgacgcgcc gcccgcatgg gagaaccttc ccctgtgggc tgttcattcg
     2161 attctgtacg atcacgggat ccacgatctc tccgcagacc aggcaacgaa aggcgcgaaa
     2221 ccggtctgcc ttcatcccac attgccagtc ctgaaactcc tcggcgtgca tgagcccttc
     2281 acagcgggca cagttcatga tgcagttcct ctgacagtcg cgtgggcacg atcgactcgg
     2341 ccggctgaac ggtgaccacc tgttgtaccg gtgacgggag cactcggccg ataagcctgc
     2401 gccaatgcaa tcagataccc gaaccgggaa ggatgctgca gtgtgacctg ggcattccgg
     2461 ctgagccggt caacggcgct gaacacctgg ttccatgtat aggcaggtaa atgggtgacg
     2521 atctcctcga aggaacaggg tcctcgacgt tctaaatcct gcagaatgtc cttttcgacc
     2581 tcacctgtgc ttgccatggt cacctccccg gcgccctgcg cttgatggtt cgtgcagttg
     2641 caacatttcc gcgcatgaac gatgtcttcg cagctgcatc aggtctcatg gatggcattt
     2701 ctacctgacc ggccgaagct gcgctatcgg tcatggtccg attctgtcat cgaactctca
     2761 aatggtctta ttgtcgtggt ttgcactaca agacgaagag cggacaggcg aacggggggg
     2821 aacgtcagga aacgagcccg cgttgaaggg cgtagcgggt caattcagca ttattcttca
     2881 tgttcatttt ttcgagaatg cgcgtccggt acgtgctgat ggtcgtgacg ctgagattca
     2941 caacgccggc gatctcagaa acggttttcc ctgaggcgat caaacacaag acttcatact
     3001 cacgatcaga cagttgttca tgcggcgcgc cggcctcccc gactttcatc ctgagagcca
     3061 acttttctcc cagcgacgga ctcacgtact gtccgccttc cagcaccttc ttcacggccc
     3121 ggaccaattc ctcaggcgcg ctggcttttg tcagataacc gtcggcgcct gccttgaaca
     3181 tacggatcgc atattggtct tccgggtgca tgctcatcac gagaacggga agcttcgggt
     3241 gttcggcttt taactgtttg agcgcgtccg gtccgcttct cccgggcata ctgatgtcca
     3301 tcaccacgat atcccatttc ttctgtctgg ctaattcgag catctcgtgc gcattgccac
     3361 cctcgccgaa cttcacggtg ccaaatccct caacgaggat atccttcacc cctcgacgaa
     3421 agaaagggtg atcatccaca acaaggattc ttaacatggg agaactccca agccattacg
     3481 ccgctggcgt ccggacgggt aaccgcatcg taatcgtcgt acccttctcc ggacttcccg
     3541 tgattgtgac ttccccgcct aacagagctg ctcgctctcg catcccgagc aggccgaatg
     3601 accgcggatc gctgattttc tgatcaggaa tgcctttccc gtcatcactc accgacagga
     3661 ggagagaacc gccttgctcc tgtaaggaaa cgcgcacggc ggtcgcgtga gcgtgacgcg
     3721 ccacgttggt gagtgcttcc tggcaaatgc ggaacaccgc ggtcgctcgc tccggctcaa
     3781 cctcgatgtc ctctgcggtg gcggtacagg tacaggtgat gccggtcagg cgctgaaact
     3841 cttgactctg ccattcaatc gccgccacca acccaagatc atccaacacc ccgggcctca
     3901 gctcgctgac aatccgttgg acatcggtaa tggtaccttc gacttgctcg atcatggccc
     3961 gaatcttctc actcagtttg cggcgaagcc tcgaaccctc cacctcgctt acaatcgtgt
     4021 tcgcttgaga gaggtcgatc ttgagacacg tgagcccgac ccccagctca tcgtgcaact
     4081 cgcgtgcgat gcgagcgcgt tcttcctctc gaaccacttc caggcggccc gacaaggtac
     4141 gcaggcggtc caatgactgc tgcagttgct cttccgcttc cttccgctgg ctgatgtttt
     4201 ccaccgtgcc ttcatatccg acaatcgctc cgttccggtc gcgtaccgcg cgcacattct
     4261 ctgaaaccca tatcacaccg ccgtccttac gacgggcttg cgattcgaac ccgatgacca
     4321 tctcctcctc atgcatccgg cgaagaaact cgtcccgtcg cctgggttcg acatagactt
     4381 gtttcgcgat gtcggtgacg gcggatatca tttcctcagg cgactcgtat ccatacaggc
     4441 gtgcgagcgc gggattcaca accagatatt tcccgtcgat cgtggtgcgg aagatccctt
     4501 ccaccgcatt ttcaaagatg ctccgatacc gttcctcggc ttgtctgatc gcctcttctg
     4561 cctgtttgtg ctgcgagata tccgtaaagg acaccactgc gcctttgatt atgccctcct
     4621 cgacgatcgc agaggaggaa tagtcaaccg ggaagtggga caagtccttg cgccagaaca
     4681 cttcgccgtc aacatgactg gcccggccgc ttcgaaacac ctggaaaatt tgacactcgt
     4741 tgacagggta cggtgtgcca tcggcacgat gatgatgaat caggtcatgc atattctttc
     4801 cgacgacttc actctgttca tagcccaaca ttttcgctgc tgctgtattg atgaaggtgc
     4861 accggccatc cagatcaagt ccgtagatgc catcgctcgt cgactccaac agcaacaacc
     4921 tgtcgttcaa caacttccgg cgctcctcgt ctgcccgctt ccgctcagtg atgtcggcgg
     4981 taatgccgca aatggcgtag agtttcccgt ttccattgtg cagcggaaac ttgaacacaa
     5041 tgctcgtatg aggaccatca tcttgcagcg caacctcttc gaactgcagc ggaactcccg
     5101 ctcgaagaac catgagatcg ttggcacgaa aagcggccgc ctgctctgat gggaagacct
     5161 cctcgtccgt tttgcctgcg attgcctcac ggctcatatg gaaggtgcgt tcgaattgac
     5221 gattgacatc cagatagcgg ccctgcgtgt ctttcagaaa cacgaggttc gggctgttat
     5281 cgagaatggc ccggagtcgc gcttcgcttt cccggagtgc tcgctcggtc cgttggcgtt
     5341 cactgatatc cctgaacatc accaccgcgc cgaccatctc gcctcgctct atgatcggag
     5401 tgctgctgta ctcaaccggg aaactcgtcc catcctttct ccagaacaca tcatcggcca
     5461 tgtactgaac cgttccatca cgaatcgtcc ggtggatcgg gcattcttct acggagtagg
     5521 tccccccatc ctgtttcgta tggtgcacga tgtcatgcat gcaacggccg accagttcct
     5581 cgcttgaaga cccgatcatt cgcgcagcgg cggggttcac gaatatcgca tgtccctgca
     5641 tatcgatgcc gtaaatccca tcatgggctg aggtcaacag aaggacatag cgatgccgga
     5701 gccgctcgag agcctcctcc acccgtttcc gttcagtaat atcacgcgca attcccaata
     5761 cgccgcaagg ccgcccgttc tggatgtggg gcaccccgac gaattctccc acgacatagc
     5821 cgcgatcctg tgagcgaatg cggaagacac aggtgagtgc ctctcctgtc tccacgatgt
     5881 gtcggaagat atctatcgcg agaggcagat cctcagggtg aagaagttcc ccaaatggtc
     5941 gtccgaccca ggcatcactg gtccattgtg taattctttc aaacgccggg ttcagcgagg
     6001 tgatgacccc ctctttggag agcgtgaaga tgacgtcccg tgccccttcg acgagggtgc
     6061 ggtaccgttc ttctgagtcg cggagggctg attccgtccg atttcgttcg gccgcgtagc
     6121 gcagcgagcg catcaacaga tggccggtca cctgaccttt gaccagatag tcctgcgcac
     6181 cggcctggat caaccgcatc cccagcgcgt catcctcgat gctggtcagg acgacgatcg
     6241 ggatgccctt cgccgcagca cgcatgcgaa tcagcgtatc catcccgtgg ctgtcgggca
     6301 gcgagagatc cagcaggacg gcatcaaaac cggcttcggc cacatgcgta agccccttct
     6361 cgagccggtc cgcgtgcaca atctgaaatc cccgggtagt gctggagtct ttcacaagat
     6421 attcctggag caggcgaata tcaccgggat tgtcctcgac aagcagaatt cgcatggaga
     6481 tcccttacac tgacctgtgt ttcccgttct tcggcagcac gacaatcccg agccagaagt
     6541 cctcaatcga cttcactacc gtgatgaact ggtccaaatc aacgggcttg gtgatgtaac
     6601 agttggcatg gaggtgatag cttttcaaca catcctgttc gtctttcgac gtcgtgagaa
     6661 caaccaccgg aattcgcctc aggctctcat cggccttgat ctcggccagc acttcccggc
     6721 catcttttat cggaaggttc aggtcaagca ggatcaggtg tggacgagcc gcattggcat
     6781 gagggccctc ccggcgtaga taggccatcg cttcgaggcc gttcttcatc acggtcagtt
     6841 tattgatcac cttggtctct ttgagcgctt ccatggtcaa gcgcacatcg ccgggattgt
     6901 cttcaattaa gagaatctcg atcggtttca caagttcgct cacggacatg ttgctgtgcc
     6961 tcctctgtct tgatgagaac ggttatgtat tgagaaaagg gagctccaaa tcgattgcgc
     7021 cggtcaactc atgctgtctc tcctctgttc gattgggata gtgaaaaaga aggttgcccc
     7081 tcggcccggc tgtgattcca cccagatatt tccgccatgg cgttccacaa tcttcttgca
     7141 cagcgccagg ccgatgccgg taccgggata ttcttctcgg tcatgcaggc gctgaaagat
     7201 gacaaagata cggtctgcga actgaggatc cactccgatg ccgttatcac ggaccgagaa
     7261 caaccattca tcgttccggc gctcagccgc cacgtgaaca ctggggggct ctgctccatg
     7321 gaacttgatg gcgttgctga ccaggttctg aaagagctgc gccaattgtc cactgtcggc
     7381 catcaccttg ggaagagcat cacgggtcac gacggcgcga ctttcctcga tggccagccg
     7441 taacgtgctc agcacaccct ccagcagctg attgcagtcg accaactcaa aggctttcgc
     7501 ttggctggtc acccgcgagt aggccaagag gtcgttgatc agccgttgca tacgggtggc
     7561 gccgtctacc gcataggcga tgaattcgtc cgcatcagca tcgagtttgc ctttgtagcg
     7621 ctttgcaagc agctgcgtat aacttgccac catgcgcaac ggctcttgca agtcatggga
     7681 cgcgacatag gcgaattgct gcagatcggc attcgaccgg atcagctcct cattcacctg
     7741 ccgcagttga ttctcggctc gttgaacctg ctgcaacagc aacgtctgat caatggcctt
     7801 gcccgcatga gaggccagag caatgatcat ttcctcgtcg agttgggtaa actcgtcggc
     7861 gccctgcttc tccgtcaggt aaatccgtcc aaaaactcgc tcgtgggtcc tgatggagac
     7921 gcccaggaag gaacgcatcg ccggatgatg cgatggaaat ccgatggcag cctggtgttc
     7981 catgaggttc ttgagccgaa gcgcgccttc ttgatgcgat aacagcccta aaatgcctcg
     8041 cccagtcggg agagctccga ttgcagtttt cgttgcctca tccatccccg aagtgataaa
     8101 tcgagcaagc tttccctgtg cctcgtcaac gattcccagc gcggcataac gagctcctgt
     8161 cacttccctc gccccgtgaa ggatctgctc gagaaggcgg tccagctcgg gactcgccgt
     8221 cacttcccga actgccccga caagggagtg gagggccctc gcccgctcct cgctgatctg
     8281 aatggactcc gccagatccc gctgggctac aagcaggtca cgttgttctt gagccactct
     8341 gcgcgccgcc catatcccga atcctgtcaa tggggccacc aacaacagcc ccactcctcc
     8401 gaccgtccag attaatcgat tgatcggggc ataggcccga tcccggtcca accgtaccag
     8461 gagcgtccag tcgaacccgg gaaacttgtt gtagcctcgc gttctggcgt atccagttac
     8521 cacgggaacc tgccggcgct gatgcagttc ttccacaaaa cccgattggc tgcggtccgc
     8581 ggctgccctg accacggaag gtagtgcctc cttcagagca ccgccggtca tgccattcgc
     8641 ttgttccttc tcactgatga tgacccctcg actatccagc agcagccagt cgaacgcttc
     8701 ttccctgtat cgcagcgctc cctcctgctg aagaatggcc cgtaggttct caagcggtac
     8761 ctgagtcgcc accgctcctt gaaattctcc gtgtggcccg aggatcggag ctgagaaccc
     8821 aacagccatg actccaccag actcccctga gggtcgcgcc tccgcgagat ggatctttcc
     8881 cgtctgtcgc acgatttcaa accagtctcg ttgatcggcg gacacgcctg atccggaact
     8941 ctcatcgctt tccgcattct tgcttcgcga gacggataaa tctgtggccg cgacaagtcg
     9001 tccggaagcg tcggcaaccc caatccagga gtaataccag tagagccttc tgtattcttg
     9061 taatcttttc tctttctcat cccggcttcc ttggagcagt attcggtcgt tggcaaacac
     9121 ctggatgtcg ccaaatcgtt caaagagcac gcggtcaagc gtgtccgcgg cctgtgctgc
     9181 cgctctcgcc agatcggctc ctcgttcgct gaccaacaca gctcgcaaaa ctcccagtgc
     9241 atggaggcca agcccaactg caatcgcaaa gcacaacaga agcactgcgg gaagaaagcg
     9301 accgtatctg gttggattca tggtctgata cgtcccgaga gtccggtcaa gcgttctcgg
     9361 tctccttacg ccttcaatcc gcttcctgtt gcggcagaac ggaaaggtct atcggcaccg
     9421 gttaacacaa tgacgtggcg cacatctgac cagtgcgaag gcaggctcgg gaagggacct
     9481 attcctcgaa ggaccaagat ccgtggaaat ggaccgcaga gaaagaggga gccgatgcgc
     9541 ttattgatcc tgcgcaaagt ccgcatgcca gtacttagaa aaaggggctt tgatcactac
     9601 cgagaggtaa tgtggcgaga gaatagcagt cttcatgatc tgagacaata gacacctcat
     9661 tgagtcccac caccaaccgc ccatgaagtc attggaggga ctgtcacctg tgtttttaag
     9721 gagcacccgc caatccggcc cgctcagcta cggcaagaca cgcacgattc acgcctcatg
     9781 gtttgatgta aacccagccc tgttcttgac gttccatcag ccggaccatc gccggcactg
     9841 tatcctcaac ctccccgatc aaatcatctc gggtgatctt catggccttc atcgtgttgg
     9901 aacaagcggt ataatgaact ccatagtccg ctttcaatat ggctagctct tgtccgagag
     9961 cggtcgcgtt tttcgtgaac aaggagagcc cagctccgtg caccaccaat tcgagctgga
    10021 gctgctcacg ccccaattcc tgatagagat gacggatatt gttcaatgtg cccttttgca
    10081 tctttccgtc gccagaattc agatgcatca cgacacgatg agacctgtcg gctccgcctt
    10141 tgtccgagaa cccctcagcc tgcactgacg gaacgaccat cacaatcgcg accagacaag
    10201 ctgccgggac aatcctgttc cacatgtgac actcctctcg cgaacctaag atgatccgcc
    10261 gctgagctgt cgactcagtt gcgtcaatgc ccagagcagg aacacaccgt acacgcagcc
    10321 gaagaaccag ggaaagctga cggcgcgatg ggcccaccac tgccggtgct ggcgaaacgc
    10381 ccagttcggc ttgaagaccg gcgggtcatt ctgcagatcc tcaaccggtt gctgtgtttc
    10441 gtggagccga tagagccccg tgatgagttt atgtttgttc cactcctgag cctgttcaat
    10501 gcccctcaac tgccactccc acagtgcatt ctggcctgaa aaacgaccga ggacgatcgc
    10561 catttggaga cagaggagaa ttccaaagac gcacaagagg agaatcagca cggtcaagga
    10621 caaggccggc gactccctgg acacagtcag accgatgacc gcgacgaaga tggagttggc
    10681 ggtcaggtag tcggagagca tcgagtgata atccctgacc ccggaagtcc agagcgtcag
    10741 cagatgatcg taggactgct cgagattccg ctccgcacca tcttcacgat cagacatggg
    10801 tcccccgtcc ggactcagcc gtggcgcaga tagtccccgg ccaatcggta ggcattgtgt
    10861 aaatattgcg tcaccatgcg ctggctgttg aagaatccgg cgttcaccgc gatggcatgg
    10921 cgcataatct ccatgaagcg ctcgtggtct tgataaaaac acgggatgac cttctcctcg
    10981 agtttgcgat acagatccac agcatgctcg gcatccacgt cgactctggg tttggatgaa
    11041 tcgacccggt cgccgatcga ccatccggtc acgtcttcca cgtggccttc gatccaccag
    11101 ccatcgagca cgctgaggct cgggactccg ttcaccgctg ccttcatccc gctggtcccc
    11161 gaagcctcca tcggaggaag gggcgtgttg agccacacat cgaccccggc acacatcaac
    11221 ttggccaggc tcatgtcata attgccgagg tacgagaccg cgatgacgcc tcgcaacgcc
    11281 tcacggatct catgaatgtg atggatgacc tgcttgccgt cctgatcggc tggatgggcc
    11341 ttgccggcga agacgagttg aagccgcccg actcgttgag caatggcctt gagtcgttcg
    11401 atgtcccgga caatcagatc cacgcgcttg taggcggtcg cccgacgcgc gaagccgatc
    11461 gtgaacacgt cgtgttcgaa tccggtgttc gtttcccgat tggcgtactc gatcaaggcg
    11521 cgtttcgctt cgagatgcgc atcccagatc tccgacgcag gaatgctcac ggcatatcgg
    11581 agcgagagtt gatcgcggcg ccaatccgga agatgccgat cgtagagctg ttggaacgat
    11641 tgtgccgccc acgtcgctgc atggacgccg ttggtaatgc tgtggatcgg atagccgggg
    11701 aagaggttgt gcgacacctc cccgtgcttc atcgccactc cattgacgta gcgagaaccg
    11761 cgcaacgcga gctgggtcat gttgagacga ctgtcctgcc cacaggcctg gagccatccg
    11821 cagcggcgtg gtccgagcac acggtgggcc agatcgggag gaaattgatc gtgcccggca
    11881 gctaccggag tatgggtcgt gaagacgcac cgttcgcgga tcgcgtcgat caggtcgctc
    11941 ggaaccgcat caccctgggc gcgggaggcc agcttgtctt ccaacagcgc cagcacgagc
    12001 aaggccgcat gtccttcatt cagatgaaac cgacggacat cgccgtaccc caacgccttg
    12061 agcatccgat agccgccgaa tcccagaacc atttcctgac agagtcgata atggtcgtct
    12121 cccccgtaca gcacatcggt caatgtccga tcccacggtt gattgtcgct taagtccgtg
    12181 tcgagaaaat acacgggcac ttcgcctcca gactcgcccg tgacgcgaca actccaagcc
    12241 cgaacctgta cgatacgatc ttcgatctcc accgtcacgc gctgatccac cggcttcagg
    12301 aaatcattga tcggccaggc aaccggctcc tcggtctgcc agccttgcgc atcgaggcgc
    12361 tgatagaaat aaccgcgccg gtggaggagt gtgaccgcca ccatgggcac atccaaatcg
    12421 gcggcggacc ggatcgtgtc gccggccagc acgccaagcc ct
//
LOCUS       DATIFR010000093         5948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1971887, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000093 DATIFR010000000
VERSION     DATIFR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971887"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VLD60_05300"
     CDS             <1..618
                     /locus_tag="VLD60_05300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HSB44406.1"
                     /translation="PKQHDIRFIQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIG
                     GIELSPITGEITYGTERIAMYLQQVNNVFDLAWTDQIKYGDIHHETEVQFSRYNFEQG
                     DVGMLMTAFQAYEAECKRLLAQTDTRLTLPAYDYCIKSSHVFNLLDARGAISVAERTG
                     YIGRVRALARQCAERYIEERAAMGHPLIERNAAAVARPKAGSGRR"
     gene            629..2797
                     /gene="glyS"
                     /locus_tag="VLD60_05305"
     CDS             629..2797
                     /gene="glyS"
                     /locus_tag="VLD60_05305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSB44407.1"
                     /translation="MKKSTVKKSRQKTARSTAGAELLLEIGSEELPYEFIAPALASLK
                     EHAALLFKEARLTTGSIQTYGTPRRLVLVVEELSIHQASVVKEAMGPSKAVAFGQDGQ
                     PTKAAIGFAAGQAVPVERLQVRQTPKGEYLFAVKQDAGRPTKTVLQELLPQLVAKLSF
                     PKAMKWNEAGVRFARPVRWLVALFAGGVVPVDVAGIKSGNRTYGHRVLGGGKPITVRD
                     YQSYRQGLERQGVMVEPDRRRAAVQEQLERLCAKVGVRLNVDESLLDQAVFTTEWPYS
                     VVGTFKPEYLAMPPEILMTSMKEHQGFFSVRDRKSGSLAAHFIAVANNQLKDMSLIRA
                     GNERVLAARLADAKFFFDEDRKSKLEDRVKKLGGVTFQQKLGTMAQKQERIRKLANMI
                     AARLSVPEEVVRACDRAGALCKADLLTGIVGEFPELQGIMGGEYAKHDGEPGAVSQAI
                     REQYLPRALEGALPETLAGQVLSMADRFDSIAAFFHVGMMPTGSEDPFALRRHATAIV
                     RIILEANLRLDLGPVIDQARAAVREQGFKAAQDTEAQGKRRITEFVFERVRHYGRTVH
                     GLRDDVMDAVLRSASISDVDLVDLLLKMKALQGVTTRPEFDPLIVGFKRASRIVEKEQ
                     WDRKPVDPGLFKDPAETELYRQTGACRKEFDKQMGKGHYDHALDALVRLKPAIDAFFA
                     GVMVNAEEAVLRSNRLSLLKEVEEFFRSFADFSRIVVQGS"
     gene            2815..3324
                     /locus_tag="VLD60_05310"
     CDS             2815..3324
                     /locus_tag="VLD60_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44408.1"
                     /translation="MAQVEPLATDLPPDDERAAKVRRFSMVVTVGMIVICNMLLLFGV
                     WVSGVNLDELVKTPDLFDSKQDICLRLTWQQVAGAAEPVRLCSEWINLADPSGKPHLL
                     AKDVKVRQGPDGRYYFDHAVRADFRLLGLALFVGAVLLFGMWVRRYLVGRYRVQLGMA
                     APNSSICLP"
     gene            3351..>5948
                     /gene="ppdK"
                     /locus_tag="VLD60_05315"
     CDS             3351..>5948
                     /gene="ppdK"
                     /locus_tag="VLD60_05315"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSB44409.1"
                     /translation="MAKKYVYYFGDGKAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYKQGKKYPPGMWEAALQSLKRVERSMGMGFGDPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVSMYGSIVMGVPREHFEAI
                     LKHKKAEVGVAHETHLDARHLRELVASFKALVKEETKKEFPDEPLDQLRMAVNAVFSS
                     WFGARAITYRRLYGIPDSWGTAVNVVAMVFGNMGETSGTGVAFTRSPSSGDKAFFGEC
                     LMNAQGEDVVAGIRTPLPVSALAKNVPEAYKELEHTYKKLEKHYRDMLDLEFTIQEGK
                     LYMLQTRVGKRTGIAAVKIAVDMVKEGLISKAEAVQRIGPDQLAQYLYPIFDTKEESK
                     SNPLGKGLPAGPGAAAGKIALTPDRAVDMKAAGNRVVLVRQETSPDDIHGMNAAAGFL
                     TARGGMTSHAAVVARQMGKVCVAGCDSVEVLDSQTVKIGAKTFREGDYLSINGSTGNV
                     YEGDIPVVESEIIQVIQGKLNPKKSDKYQRFATILSWADSFRKLHVRANADVPDQANI
                     ARGFGAEGIGLCRTEHMFFAEDRIPIMQKMILARTKEEREKYLDQLLPLQKQDFVGLY
                     REMKGHPVTIRLLDPPLHEFLPKREELMVEIAQLELTGKDAAKLEEQRRLLARVEELH
                     EFNPMLGLRGCRLGITMPEITRMQARAIMEAACEVTKEGAKIVPEIMIPLVGMVSEMK
                     SQKDIVREVAQETMKKYNVKLSYLVGTMIELPRAAVTADQIAKEAEFFSFGTNDLTQT
                     TFGFSRDDAAKFIDFYRTANIMDADPFAVLDREGVGSLMKQAIAAGRTTRPGIKLGIC
                     GEHGGDPSSV"
BASE COUNT         1280 a         1525 c         1925 g         1218 t
ORIGIN      
        1 cccaaacagc acgacatccg gtttatccag gacgattggg aatcgccgac gctcggcgcc
       61 tgggggctcg gctgggaagt gcgcctcgac gggatggaga tcacccagtt cacctatttc
      121 caggaaatcg gcggcattga gctgagtccg attaccggcg agattaccta tggcacggaa
      181 cgcattgcca tgtatttgca gcaagtgaac aacgtgttcg atctcgcgtg gaccgatcag
      241 atcaagtacg gcgacatcca ccacgagacc gaagtgcagt tctcgcgcta taacttcgaa
      301 caaggtgatg tcggcatgct gatgaccgcg ttccaggcgt acgaagctga atgcaaacgg
      361 ctgctcgcac aaaccgacac acggctcaca ctgccggctt acgactactg tatcaagtcc
      421 tcgcatgtgt tcaacttgct cgatgcgcgc ggagcgatca gcgtcgcgga gcgaaccggt
      481 tacatcggcc gtgtgcgtgc gctggccagg caatgtgcgg aacgatatat tgaagagcgc
      541 gcagccatgg ggcatccttt gatagagcgg aacgcagccg ctgtcgctcg ccctaaggcc
      601 ggttctggcc gcaggtaacg caccacccat gaagaaatca acagtaaaga agtcccggca
      661 gaagactgcg cgttccaccg ctggcgcgga gttgttgttg gaaatcggtt cggaagaact
      721 tccttacgaa tttatcgctc ctgcgcttgc atcactcaaa gagcatgccg cgctcctatt
      781 caaggaggcc agactcacga ccgggtcgat tcaaacctat ggaacacccc ggcggttggt
      841 gttggtggtc gaagaactta gtatccatca ggcgtctgtc gtgaaagagg cgatgggccc
      901 gtcgaaagca gtggcgtttg gccaggacgg ccaaccgacc aaggcagcga tcgggtttgc
      961 cgccggtcaa gctgtgccgg ttgaacgtct gcaagttcgc caaacgccca agggcgagta
     1021 tctctttgcg gtgaagcagg acgcgggtcg tccgaccaag acggtcttgc aggagctgct
     1081 gccgcaactt gtcgccaagc tgtcctttcc gaaagccatg aagtggaatg aggcgggcgt
     1141 gcgttttgcc aggcctgtcc gatggcttgt ggcgctcttt gccggcggcg tcgtccccgt
     1201 tgatgtggca ggaattaaga gcggcaatcg gacctatggt caccgtgtgc tggggggcgg
     1261 gaaaccgatt acggtgcgtg actatcagag ctatcgtcaa gggcttgaac gtcagggcgt
     1321 catggttgag ccggatcgcc gtcgggccgc ggtccaagaa cagttggaac gcctctgcgc
     1381 caaggtcgga gtgcggctga acgttgatga gtcgttgctg gatcaagcgg tatttacgac
     1441 ggagtggccc tattcggtag tcggaacgtt caagcctgaa tatctggcga tgccgccgga
     1501 aattttgatg acttcaatga aggagcatca ggggttcttc tcggtgcgcg acaggaagag
     1561 cggatcgctg gcagcgcatt ttattgccgt ggcgaataac caattgaaag acatgtcctt
     1621 gattcgagcg ggtaatgaac gggtgctggc tgccagactg gcggatgcca agtttttctt
     1681 cgacgaagac cgcaagtcga agttggaaga ccgcgtgaag aagcttggcg gtgtcacgtt
     1741 ccaacaaaaa ctcggcacca tggcacagaa gcaagagcgc atacggaagc tggctaatat
     1801 gatcgcggca cgattgtcgg tccctgagga agtcgtgcgg gcctgcgatc gagctggcgc
     1861 tctttgcaag gccgatctgc tcacggggat cgtgggggag tttcctgagt tgcagggtat
     1921 catgggcggc gagtatgcga agcatgatgg ggagccgggg gccgtcagcc aggccatccg
     1981 tgagcaatac cttccacgag ccctcgaagg cgctttaccc gagacgctgg cgggccaggt
     2041 cctctcaatg gccgaccgat tcgactcgat tgctgcgttt ttccacgtcg ggatgatgcc
     2101 gaccggatct gaagatccct ttgcgctccg tcgccacgcc actgctatcg tgcggatcat
     2161 tttggaagcc aatctgcgtc tcgatctggg acccgtgatc gatcaggccc gagcggccgt
     2221 gcgcgagcag ggctttaaag cagcccagga tacggaggcg caaggaaaac gacgcatcac
     2281 agaattcgtc tttgagcgcg tgcgtcacta tggccggacc gtgcacggac tgcgtgatga
     2341 tgtgatggat gcggtgctgc gatcggcttc gatctccgac gttgatctgg tcgatctgct
     2401 cttgaagatg aaggcgctgc agggcgtcac aacgaggccg gagttcgatc cgttgatcgt
     2461 ggggtttaag cgggcttccc gcatcgtcga gaaggagcaa tgggatcgga agccggtcga
     2521 tcctggtttg tttaaagatc ccgccgagac tgagctctat cgacaaacgg gggcgtgccg
     2581 caaggagttc gataagcaga tgggcaaggg ccactatgat catgcgctgg atgctctggt
     2641 tcgcctcaaa ccggcaatcg atgccttttt tgccggtgtc atggtgaatg cagaggaggc
     2701 ggtgctccgc agcaaccggc tttcactcct gaaagaagta gaggagttct ttaggtcatt
     2761 cgccgacttc tcgaggattg tggtacaagg gagttgagcg gagcatgtag gccgatggcc
     2821 caggtcgaac cactcgcgac ggatctgcct ccggacgacg agcgcgcggc aaaagtacgc
     2881 cggttttcaa tggtggtgac ggtggggatg atcgtcatct gtaacatgct cttgttgttc
     2941 ggtgtctggg tcagtggcgt caatctcgat gagttggtca agactcccga tctgtttgat
     3001 tctaagcaag atatttgttt gcgtttgacg tggcagcagg tggccggggc tgccgagccg
     3061 gtgcgactct gttcggaatg gattaacctg gccgatccgt ccggcaagcc ccatctgctg
     3121 gctaaagacg tgaaggtgag gcaggggccg gacggtcggt actatttcga tcacgcggtt
     3181 cgggcggatt ttcgattgtt agggctcgcg ctgtttgttg gagccgtact gttgttcggg
     3241 atgtgggtgc ggcgctatct ggtcggccgc tatcgggtac aactcggcat ggcagcgccc
     3301 aacagttcaa tatgtctccc ctgagggtgg aggagaagga gagggcaggc gtggcaaaaa
     3361 aatacgtcta ctatttcggc gacggcaaag ccgagggcac atcgaatatg aaggaattgc
     3421 tcggcggcaa aggggcgggg ttggcggaga tgaccaacct gggcatctcg gtgcctcctg
     3481 ggttcacgat ctcaacggaa gcctgtgttg aatattacaa gcagggaaag aaatatcctc
     3541 cgggcatgtg ggaggccgcc ctccagtcat tgaagcgcgt cgaacgctcg atggggatgg
     3601 gtttcggtga tccggaacgg ccgctgcttg tctctgtccg atccggtgcc cgcgcgtcga
     3661 tgcccggcat gatggatacg gtcttgaatg tgggtctcac gaccaagacg gtcgagggac
     3721 tggcggcaaa aacgcggaac gaccggttcg cccaggacag ctatcgccgg ttcgtctcca
     3781 tgtacgggag catcgtcatg ggggtcccgc gagaacattt cgaagccatc ctaaagcata
     3841 agaaggccga agtcggcgtg gcgcatgaga cacacttgga tgcccggcat ctccgcgagt
     3901 tggtggcgag ctttaaggct ctggtgaaag aggaaacgaa gaaagagttt ccggacgagc
     3961 cgctcgatca gttgcgtatg gccgtcaacg cggtgttttc ctcctggttc ggtgcgcggg
     4021 cgatcaccta ccggcggctc tacggcattc ccgattcctg gggtactgcg gtgaacgtgg
     4081 tggcgatggt gttcggcaat atgggtgaga cgagcggaac cggcgtcgcc tttacccgaa
     4141 gcccgagctc aggcgacaag gcgttcttcg gtgaatgtct gatgaacgca cagggcgaag
     4201 acgttgtggc ggggatcaga acgccgttgc cggtcagtgc gctcgcgaag aacgtgccgg
     4261 aagcctacaa ggagttggag cacacctata agaagctcga aaagcactac cgcgacatgc
     4321 tcgacctgga gtttacgatt caggagggca aactctacat gctgcagacc cgcgtcggga
     4381 agcgcacggg gatcgcagcg gtcaagatcg ccgtcgatat ggtgaaggaa gggctgatct
     4441 ccaaagcgga ggcggtgcag cggattgggc ctgatcagtt ggcccagtat ctctatccga
     4501 tcttcgatac gaaggaagaa tccaagtcca atccgctcgg caaagggttg ccggccggtc
     4561 ccggcgcggc tgcagggaag attgctctca cgccggatcg ggctgtcgac atgaaggcgg
     4621 caggcaatcg tgtcgtgttg gttcggcaag agacgagccc agatgatatt cacggcatga
     4681 acgcggcggc cggatttctg accgctcgcg gcgggatgac ctcgcacgct gcggtggtgg
     4741 cccggcagat gggcaaagtc tgtgtggccg gatgcgattc ggtggaagtt ttggacagcc
     4801 agacggtgaa gatcggtgcc aagacgttca gagagggcga ttatctgtcg atcaatggat
     4861 cgaccggtaa cgtctacgag ggagacattc cggtcgttga atccgaaatc attcaagtga
     4921 tccaagggaa gttgaatccg aagaaatcgg acaagtatca gcggttcgcc acgatcttat
     4981 cctgggccga tagttttcga aagctgcacg tgcgggcgaa cgccgacgta ccggatcagg
     5041 caaacatcgc gcgggggttc ggggcggaag ggatcggcct ctgccggacg gagcatatgt
     5101 ttttcgccga ggatcgtatt cccatcatgc agaaaatgat cttggcgcgg acgaaagagg
     5161 aacgtgagaa atatctcgat cagttattgc cgctccaaaa acaagatttc gtcgggctct
     5221 atcgcgagat gaagggccac cccgtcacga ttcgtttgct cgatcctccg ctgcatgaat
     5281 tcttgccgaa gcgggaggag ttgatggtcg agatcgccca gctcgaattg acggggaaag
     5341 acgcggcaaa gctcgaggaa caacgtcggt tgctggcgcg cgttgaagaa ctgcatgagt
     5401 tcaaccccat gctcgggttg cgcggctgcc ggctcggcat caccatgccg gaaatcacca
     5461 gaatgcaggc gcgggccatc atggaagcgg cctgcgaagt taccaaagaa ggcgcgaaga
     5521 tcgtcccgga aatcatgatc ccgctcgtcg gaatggtctc ggagatgaag tcccaaaagg
     5581 acatcgtgcg agaagtcgca caggagacga tgaagaagta caacgtgaag ctgtcctatc
     5641 tcgtcgggac gatgattgaa ttgccgcgtg ctgccgtgac cgccgatcag atcgccaaag
     5701 aggccgagtt cttttcattc ggaacgaacg atctgacaca gacgacattc ggattctccc
     5761 gggacgatgc cgcgaagttc atcgattttt acaggacggc gaacattatg gatgcggatc
     5821 cctttgccgt tctcgatcgg gaaggtgttg gatcgctgat gaagcaagcg attgcagccg
     5881 gccgtacgac gcgccccggc atcaagctcg gtatttgcgg cgagcacggg ggtgatccca
     5941 gttccgtg
//
LOCUS       DATIFR010000094         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_821622, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000094 DATIFR010000000
VERSION     DATIFR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821622"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..141
                     /locus_tag="VLD60_05320"
     tRNA            66..141
                     /locus_tag="VLD60_05320"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Asn,seq:gtt)
     gene            complement(107..391)
                     /locus_tag="VLD60_05325"
     CDS             complement(107..391)
                     /locus_tag="VLD60_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44410.1"
                     /translation="MQELEVEKQDLDARKERLELPALDRKFLSKLLDEFDETMENGTN
                     QKKKHLLRQLVKKVLIHDRRTVEIWYGLPNRASVRTPGHLAPWAGLEPAT"
     gene            complement(711..1127)
                     /locus_tag="VLD60_05330"
     CDS             complement(711..1127)
                     /locus_tag="VLD60_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB44411.1"
                     /translation="MFYLWHVIDSRKEKLARALASTRFRPSEKAMPLTLPKPVTNYLA
                     AVEAKDTDMLALCFTDDALVHDEGRDHRGLDAIRSWAKKTQRKYKYVMEPLNTSVSAK
                     FVKLHARLTGDFPGSPIELDYTFTIANDKITSLEIG"
     gene            1486..2481
                     /locus_tag="VLD60_05335"
     CDS             1486..2481
                     /locus_tag="VLD60_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB44412.1"
                     /translation="MGKALEQLKTISNEDYAKVKGLASIVKPLRSVLHKTPDDYGMIG
                     WQDIYFPSDDGTPLEGWYIPAKGGESDKLILFNHALPMSRAGFQGHFGEPWSSFDAVE
                     IDFVIQMKHLTDAGYNVLAYDLRNHGTSSAANGGICGIGRYEWRDCVGVKKYVDSQPY
                     VDSNPALSKMKLGLYSQCTGGNAQFEAIYRHPELFDDILCMCCPMVVSMGAIFSAFSE
                     LQGISQYLELIDFELIKMGAYTAAEMNPQLFASAVKMPVLMFQVLKDAWTRNPEDAQK
                     TFDLISSTDKELFWIENTTRRFRDGYNYFGRNPERIIAFFDKHMKEAATVERDAA"
     gene            2928..3317
                     /locus_tag="VLD60_05340"
     CDS             2928..3317
                     /locus_tag="VLD60_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB44413.1"
                     /translation="MANKTLLLVLLCLVSSPLMAQEPSVTPLISKDLTDRPGKEVLMI
                     IVEYPPGGSDPVHRHNAHGFLYVLEGSVVMQVKGGKEITLTPGESFYEGPDDVHVVGR
                     NASGTKPAKFLVFLIKDKGAPVLVPVK"
     gene            3430..4179
                     /locus_tag="VLD60_05345"
     CDS             3430..4179
                     /locus_tag="VLD60_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB44414.1"
                     /translation="MKIVVIGGSGLIGTKLVNNLREHGHEAVAASPASGVDTLTGAGL
                     VEALTGTQVVVDVTNSPSFEDAAVLRFFETSTRNLLSAEAAAGVRHHVALSVVGADRI
                     PDSGYMRAKFAQEKLIKAATVPYTILRATQFFEFIGRIADGSTDGNTVRLSPALMQPV
                     AADDVAAELDHVALGEPVDGMVELAGPEPIRLDELARRLLSARHDARQVTTDVHARYF
                     GAEVNDQSLTPGDNPRVGPTRFDEWLSRLAA"
     gene            4212..>4640
                     /locus_tag="VLD60_05350"
     CDS             4212..>4640
                     /locus_tag="VLD60_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB44415.1"
                     /translation="MSTITVKDGTTIYYKDWGTGPVVTFSHGWPLSSDAWDGQMLFLA
                     QNGFRVVAHDRRGHGRSSQASSGNDMNGYADDLAAVIEALDLTEVTLVGHSTGGGEVA
                     RYIGRHGTKRVAKAVLVAAVPPIMLKSAANPEGLPMEVFDN"
BASE COUNT         1037 a         1292 c         1302 g         1009 t
ORIGIN      
        1 cgcggaccaa tccgcttgac tgtccgccgc cgggggacta tattctccgt ctgccgcttg
       61 ttcgttccct ggtagctcaa ccggcagagc gggtggctgt taaccactag gttgcaggtt
      121 cgagtcctgc ccagggagcc aaatgtcccg gtgtacgaac cgaggcccgg ttcggtaacc
      181 cgtaccagat ctcgacagta cgccgatcat ggataagcac cttcttcacc agttgccgga
      241 gaaggtgctt ctttttttgg ttggtcccgt tctccatcgt ctcgtcgaac tcgtcaagga
      301 gctttgaaag gaacttccga tcgagagccg ggagttcgag ccgctccttc cgagcgtcca
      361 gatcctgttt ctcgacctcc aattcctgca cctgcgcttg gagatcccgg accttctggg
      421 agcgctggac ccgccaacgc agttcaccag gatatcaatg ccgccccagc tcttcagaag
      481 tttatccacg acctcttgca cgccatatac tgtaccgatg tctgcttgga caaacagcgc
      541 cggctgctgt ccttcgggga ggggtgaacg cgcagtagtc gcgacagaag cgctgcccat
      601 tgtcagcctg cgtacgatgg cctctcccat gcctttggtc ccgccggtca ccaggacccg
      661 tttgcctttg aattcatttc cgtcaacagg aaaagggtag gttgctgtca tcatccaatc
      721 tcaagtgagg tgattttgtc gtttgcgatc gtgaacgtgt agtcgagttc gatcgggctt
      781 ccggggaagt cacccgtcaa ccgggcatgg agtttcacga actttgcgct caccgaagta
      841 ttgagcggct ccatcacata cttgtatttc ctttgggtct tcttcgccca ggaccttatc
      901 gcatcgagcc cgcgatgatc gcgcccttcg tcatgcacca gggcatcgtc cgtaaagcaa
      961 agggcgagca tgtccgtatc ctttgcttcg actgcggcga gataattggt gactggttta
     1021 ggtagtgtga gcggcatcgc tttctcactc ggtcgaaatc gcgtgcttgc caaagcccga
     1081 gccagctttt ctttccggct gtcaatgaca tgccacaggt aaaacatctt ctccttgcga
     1141 tgccactgaa aatgggcaag attctcaacg atacccgccg ccaggtgccg gtcttccggg
     1201 ggggtggtgg ggaaacccgt gggggttcga gtcccccctt cggcaccata gcaattcaaa
     1261 aagggatttc ggcctgacgc cgaagtccct ttttttgtga agaacgcaaa tgtggctcat
     1321 ttatggctcg tgggtctttt ccgtctctca cgcagtctct tgagactcaa caggtcggct
     1381 gtcccgcccg tggcatctaa agaaacacca ggcgagcagc aaccgaacga gaagcttcac
     1441 tcgagagagg ggggagaaac gatgagtcga atatcaaaag aaagtatggg caaagccttg
     1501 gaacaactca agacgatttc gaatgaagac tacgccaagg tcaaagggct ggctagcatc
     1561 gtcaagccgt tacgaagcgt tcttcataag actccggatg actacgggat gatcggatgg
     1621 caagacatct attttccatc agatgacggc actcctttgg aaggttggta cattcccgcc
     1681 aaagggggcg aaagcgacaa gttaattcta ttcaaccatg cgctgccaat gagtcgcgcg
     1741 ggttttcagg ggcacttcgg agaaccgtgg agcagttttg acgccgtcga gatcgatttc
     1801 gtgatccaga tgaagcacct gaccgacgcc gggtacaacg tcctcgcgta cgacttgaga
     1861 aaccacggca ccagcagtgc cgcaaatggc ggtatctgtg gaataggcag atacgaatgg
     1921 cgggattgcg tgggagtcaa gaaatacgtg gacagtcaac cttacgtgga cagtaatcct
     1981 gcgctcagca aaatgaagct cgggctttac agtcagtgca cgggaggcaa cgcgcaattc
     2041 gaagccatct acagacatcc cgagctgttt gacgacattt tgtgcatgtg ttgcccgatg
     2101 gttgtttcca tgggggcgat cttttcggcg ttctctgaat tgcagggaat cagccagtac
     2161 ttggaactta tagatttcga gttgataaag atgggcgctt acacggccgc cgaaatgaat
     2221 ccacagctgt ttgcctcggc cgtcaagatg cccgtgctca tgttccaggt gttgaaagac
     2281 gcttggacac ggaatcctga agatgcacaa aagaccttcg atctaatttc aagcacggac
     2341 aaggaattgt tctggatcga aaacaccaca agacgcttca gggatggcta caactacttc
     2401 ggtcggaatc cagaacgaat catcgcgttc ttcgacaaac acatgaagga agccgcgacc
     2461 gtggagcggg acgcggcata accatttgct gaacttgacc ggggtggtgg gaaacccgtg
     2521 gggttcgagt ccccccttcg gccccattgc aatactcaaa gggatttcgg ctcgccgaag
     2581 tccctttttt ttatgaaaac gggcagagtg gctcaaaagg gactcgcggg tttcatggtg
     2641 gcgtttcgtc tgctcctgcc gcggagccaa ttcttcggtt agacgtaaaa atgaacagaa
     2701 ctcgaatcga tcatataacg agaagcacca catgaattag attcaatttt atacggggag
     2761 cccgatcggt tcccacgctg ttacgtgtac gttttcgaac ttgcgggccc cgtaaagccg
     2821 caatgggatg acaaatggct cggtcatcgg ccgcctctca tagaggtcaa gtgggtcagg
     2881 gctgccttcg tccgcccgcg caggcggcga tcaacgaaag gtcaattatg gcgaacaaaa
     2941 cgctgctttt ggttctgctg tgtctcgtat ccagcccact aatggctcaa gagcccagtg
     3001 ttacgccgct catatcgaaa gatctgacgg accgccccgg caaggaagtt ctgatgatca
     3061 tagtggagta tcctcccggc gggtcggacc ctgtacatcg gcacaatgcg cacgggttcc
     3121 tttacgtgct ggagggctcc gtcgtgatgc aggtgaaagg cggaaaggaa atcaccctga
     3181 caccaggtga gagtttttat gaaggacccg acgatgttca tgtcgttggc cggaacgcaa
     3241 gcggtaccaa gccagcgaaa ttcctcgtgt tcttaattaa ggacaaagga gctcctgtgc
     3301 tagtaccagt gaagtgaata gggaatgagc gccgtgccac ctctgaggag gaactacatc
     3361 ctactaactc tccgagaatg atctcggact tcactcgccc gttgcgggcg cccagcgaga
     3421 ggtcaactta tgaaaatcgt agtcattggc ggcagtggtc ttatcggaac aaaactcgtg
     3481 aacaaccttc gtgaacacgg ccatgaagcg gtggcagcat caccagcttc tggcgtcgac
     3541 acgcttaccg gtgcaggact cgtcgaagca ctgacgggca cccaagttgt cgtcgatgta
     3601 accaactcgc cttcctttga agacgcagcg gtgctgaggt tcttcgaaac ctcaacccgc
     3661 aacctccttt ccgcagaagc ggccgcaggt gtaagacatc acgttgctct gtcggtcgtc
     3721 ggcgctgacc gtattcccga cagcggttac atgcgcgcca agtttgctca ggagaaattg
     3781 ataaaggccg cgacggtgcc ctacacaatt cttcgcgcga cgcagttctt cgagttcatc
     3841 ggcagaatcg ccgatgggag caccgacggc aacacggtcc gcctgagtcc tgccctcatg
     3901 cagccggtcg cggccgatga cgtcgccgcc gaactcgacc acgtcgcgtt gggtgagccg
     3961 gtagatggca tggtcgaatt ggccggaccc gaaccgatcc ggctcgacga actcgcccgg
     4021 cgactcctga gcgcaagaca cgacgcgcga caggtaacca ccgatgttca cgcgcgctac
     4081 tttggcgcag aagtgaacga tcagtcgctc acgcccggcg ataacccgcg cgtcggccca
     4141 actcgcttcg acgaatggct cagccgcctt gcggcctagc agcccgaaaa ttcgattcag
     4201 gaggaaatca tatgagcacg attacagtga aagatggcac cacgatctac tacaaagact
     4261 ggggcaccgg cccggtcgtc acgttttcgc acggctggcc gctgagttcc gacgcttggg
     4321 acggccagat gcttttcctc gcacagaacg gtttccgtgt ggttgcacac gaccggcgcg
     4381 gccatggccg gtctagccag gcctcgtccg ggaacgacat gaacggatac gccgacgatc
     4441 tggcagccgt tattgaggcg cttgatctca cggaagtcac gcttgtgggc cactccactg
     4501 gtggagggga agttgcgcgc tacatcggac gccacggcac gaaacgggtt gccaaggcgg
     4561 tcctcgtcgc cgccgtgccg ccgatcatgc tcaagtccgc agccaatccg gagggtctcc
     4621 cgatggaggt gttcgacaac
//
LOCUS       DATIFR010000095         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_55571, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000095 DATIFR010000000
VERSION     DATIFR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55571"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VLD60_05355"
     CDS             complement(<1..560)
                     /locus_tag="VLD60_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HSB44416.1"
                     /translation="MKQGDLKDILQQYGVGPIQFSGTNDALYERHLTFDHVVDVKKAD
                     LREQFEAAAHSIRDIISQRWIRTEDTYAKKNPKRVYYLSMEFLLGRSLTNNIVNALLA
                     PFIAERAKKKGLDPLALVEMEPDAGLGNGGLGRLAACFLDSMATMQLPGMGYGLRYEY
                     GIFKQTIKDGWQCEQPDNWLRRPDPW"
     gene            complement(662..>2932)
                     /locus_tag="VLD60_05360"
     CDS             complement(662..>2932)
                     /locus_tag="VLD60_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HSB44417.1"
                     /translation="PRLLGHWGTTPGLNFLYVHLNRAIKKRDLSMIYIIGPGHGGPGI
                     VANAYMEGTYSEVYPNIPQDEEGMKRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYA
                     VSHAYGAAYDNPDLVVACVVGDGEAETGPLATSWHSNKFLNPATDGAVLPILHLNGYK
                     IANPCVLARISHEELDQLFRGYGYSPHFVEGRDPAKMHQLMAATLDTCLEEIRRIQTK
                     ARTHGVKERPRWPMIILRSPKGWTCPKEIDGKKTEDYWRSHQVPMGEMHEKPEHIKIL
                     EKWMKSYKPEELFDTTGRLKPELADLPPKGERRMSANPHANGGLLLRDLRLPDFRDYA
                     VKVTTPGAITAESTRLMGKFLRDTMKLNMESRNFRLFSPDENNSNRWQDAMEVTNRAW
                     MADRYPYDDHLAPDGRVMEMLSEHQCQGWLEGYLLSGRHGFFSCYEAFIHIIDSMFNQ
                     HAKWLKVCHDIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEVVRVY
                     LPPDANTLLSVTDHCLRSRNYVNVIVAGKQPAPQWLTMDQAIRHCTAGIGIWEWASTD
                     KGGDPDVVMACCGDVPTLETLAAVDLFRQHLPTLKIRVVNVVNLMKLQPQSEHPHGLS
                     DQDFDALFTKDKPIVFAFHGYPWLIHRLTYRRNNHKNLHVRGYKEEGTTSTPFDMVVM
                     NDLDRFHLVGDVIDRLPQLGSRAAYAKQAIRDKLLEHKEYIAKYGEDMPEITDWEWGK
                     VKAEKRRRTTTEGDNV"
BASE COUNT          556 a          872 c          843 g          661 t
ORIGIN      
        1 tcccagggat cgggacgccg caaccaatta tccggctgct cgcattgcca gccgtccttg
       61 atcgtttgct tgaagatccc gtattcgtaa cggagcccgt agcccatacc gggaagctgc
      121 atcgtcgcca tcgaatcgag gaagcaggct gctaaccgtc caagtccacc attgcccaag
      181 cctgcatccg gctccatctc gaccagcgcg agagggtcga ggcctttctt ttttgctctc
      241 tcggctataa agggggcgag caaggcattc acgatgttat tggtcaagga ccttccgaga
      301 agaaattcca tcgacaggta atagacccgc ttgggattct tcttggcgta ggtgtcttcc
      361 gtccggatcc agcgttgtga aatgatgtcg cggatcgaat gtgccgctgc ttcaaactgt
      421 tcacgcagat cggccttctt gacatcaacg acgtggtcga acgtcagatg ccgctcgtag
      481 agcgcgtcgt tcgtaccgga aaattggata ggcccaactc cgtattgttg aagaatgtcc
      541 ttcaagtcac cttgtttcat acaacctccg cggtgcagtt caaggaagcg gtgtctttgc
      601 cgacctggct tccttttccc gctcccaaat catcaagcaa gcaaacacat tcagttacgg
      661 ctcagacgtt gtctccctcc gtcgtggtcc tccgcctctt ttccgctttc accttgcccc
      721 attcccagtc ggtgatttca ggcatgtctt cgccatactt ggcgatgtat tctttgtgct
      781 cgagcagctt gtcgcggatc gcctgcttgg cataggctgc acgggacccg agttgcggca
      841 gacgatcaat cacgtcgccg acaaggtgaa accgatcgag atcattcatc accaccatgt
      901 cgaacggcgt gctcgtcgtg ccctcctcct tgtagccgcg cacgtgcaag ttcttgtggt
      961 tattccgccg gtacgtcagc cggtggatta accaggggta accgtggaag gcaaacacga
     1021 tcggcttgtc cttggtgaac agcgcatcga agtcctgatc ggacaggccg tgcgggtgtt
     1081 cgctctgcgg ttgtagcttc atcaaattga ccacgttgac gacgcggatc ttcagcgtcg
     1141 gaagatgctg gcggaacaaa tccacggcgg ccagagtctc cagtgtcggc acatctccgc
     1201 aacaggccat gacgacatcg ggatctcccc ccttatcggt gctggcccat tcccagattc
     1261 cgatgcccgc agtgcagtgt ctgatcgcct gatccatcgt caaccattgc ggagccggct
     1321 gcttgcccgc gacgatgacg ttcacataat tacggctgcg caggcagtga tcggtgaccg
     1381 acagcagcgt gttcgcatcc ggcggcagat agacgcgcac gacctccgct ttcttgttga
     1441 cgacgtgatc gatgaagccc gggtcctggt gactgaaccc gttgtgatcc tgccgccaaa
     1501 cgtgtgagct gagcagataa ttgagcgaag cgatcggtcg ccgccacgga atatcgtggc
     1561 agaccttcag ccacttggca tgctggttga acatcgagtc gatgatgtga atgaacgcct
     1621 cgtagcagga gaaaaacccg tgccggccgc tcaacagata cccttccagc cacccctggc
     1681 actggtgctc actgagcatt tccatcacgc gtccgtcagg ggccaaatgg tcatcgtacg
     1741 gatagcgatc cgccatccag gcgcgattgg tgacctccat cgcatcctgc cagcggtttg
     1801 agttgttctc atccggactg aacaggcgga aattgcggct ctccatattc agcttcatcg
     1861 tgtcgcgcag gaactttccc atcaggcggg tggattccgc cgtgattgcc cccggtgtcg
     1921 tgaccttgac ggcgtagtcg cggaagtcag gcagacgcag gtcccggagc agcaagccgc
     1981 cgttggcgtg aggattggcg ctcatgcggc gctcgccctt cggcggcaga tcagcaagct
     2041 cgggcttcaa ccggccggtt gtatcgaata gctcctccgg cttgtagctc ttcatccact
     2101 tttcgagaat cttgatgtgc tcgggctttt cgtgcatttc ccccatcggc acctggtggc
     2161 tgcgccagta gtcctcggtc ttcttgccgt cgatttcctt cgggcaggtc caccccttgg
     2221 gcgaacggag aatgatcatc ggccagcgcg ggcgctcctt cacaccgtgt gtgcgcgcct
     2281 tggtctggat gcggcggatt tcttccagac aggtatcgag agtggcagcc atcagctggt
     2341 gcatcttggc cgggtccctg ccttcgacaa aatggggcga gtagccgtag ccgcggaaga
     2401 gctgatccaa ttcttcgtgg ctgatacggg ccagcacgca gggattggcg atcttgtacc
     2461 cgttcaagtg caggatcggc agcacggctc cgtcggtcgc cgggttgagg aacttgttgg
     2521 agtgccagga cgtcgccaat ggtccggttt ccgcctcgcc gtcgccgacg acgcaggcaa
     2581 cgaccaggtc ggggttgtca tacgcagccc cgtaggcatg agagaccgca tatcccaatt
     2641 cgccgccttc gtgtatcgag ccgggagtct ccggcgccac gtggctggga atgcctccgg
     2701 ggaacgagaa ctgcttgaac agccgcttca taccctcttc gtcctgcggg atgttcggat
     2761 agacctcgga gtaggtgcct tccatgtagg cattggcgac gatgccggga ccgccgtggc
     2821 cgggaccaat aatgtagatc atgctgagat cccgcttctt gatcgctcga ttcagatgga
     2881 catacaggaa attcaggccc ggggtggtcc cccagtgacc gaggagccga gg
//
LOCUS       DATIFR010000096         4020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2163158, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000096 DATIFR010000000
VERSION     DATIFR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163158"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VLD60_05365"
     CDS             complement(<1..1022)
                     /locus_tag="VLD60_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB44418.1"
                     /translation="MVSRAIFVLLSALAGMALFIKAEGADASYWLYGFGIGASTGGLI
                     VAGEYALRNLSFGIIVGGTAGLAVGLLLTGLIEWVGGEIFDVQTFLFHIGGLVFLLGF
                     PYLGLVFGARFGKEKFPSGEQKFFELTGNTVCPKVLDTSVIIDGRVADLCETGFMEGT
                     FLVPHFILDELQHIADSSDSLKRARGRRGLDILNKIQKMGDVDVRIIDEDFPHVKEVD
                     AKLVVMAKKLNAKIVTNDLNLNKVAELQGVRVLNINELCNALRPVVLPGETIRVFVLK
                     EGKEAGQGVAYLDDGTMIVVDNARRYIGKNVDVVVTSVLQTTAGRMIFTRLKEEAERE
                     ELQVARV"
     gene            complement(1076..2593)
                     /locus_tag="VLD60_05370"
     CDS             complement(1076..2593)
                     /locus_tag="VLD60_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSB44419.1"
                     /translation="MKHGRSWLVPFILITVGIVIGVVVASDMGWLPTGTAGPDPIPNG
                     IVRPVATAPQMPMGGGNAKNFVEIAKMVKPAVVNIAATRSGKAGEGPQSSPFDDPFFR
                     KFFGDEFFKRDAPQRERKERGQGSGVIVDPNGLIITNNHVVNKADEIRVILSDKREFK
                     GKLIGTDTKTDIAVVKIEATGLSTIPLADSDQLEVGEFVLAVGSPFGLTQSVTMGIVS
                     AVGRASMGIAEYEDFIQTDAAINPGNSGGALVNVRGELVGINTAIFSQSGGNMGIGFA
                     VPSNLARTIMDQLVKTGKVVRGWLGVAIQELTPELATQFGLADTKGVLVSDVMDESPA
                     KKAGFERADVITEYDGKPMDSPTHLRNAVAQTPIGKKVTVKLIRDKKPKQLEVTIAEQ
                     PKSFGQPSVEEGGESTAPTGVLSDLDVRELNEELAARYGLKTTERGVIIVRVKPGSTA
                     EEMGVREGDMVLEVNRKPVSSLKSYERAVSGLTKDQAVLLLLKRKGQTIYLTLRP"
     gene            complement(2603..3526)
                     /locus_tag="VLD60_05375"
     CDS             complement(2603..3526)
                     /locus_tag="VLD60_05375"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSB44420.1"
                     /translation="MADRVQEILSGYGSDNAGTKANIGRLLRSGRLAGTGKLVILPVD
                     QGFEHGPARSFAPNPPGYNPLYHFQLAIDAGCNAYAAPLGFLEAGASHYAGQIPLILK
                     LNNHDVLHDEKDPLPSVTGSVKDALRLGCSAVGFTIYPGSSHCNAMYEQVRAIAEEAK
                     DHGLAVVVWSYPRGSALSKEGETAMDVVAYAAQIAAQLGAHIIKVKLPSAHLEQAAAK
                     KVYETEQIPIKTLAERVKHVVQSAFDGQRIVIFSGGAKSEDKNVFEEARAIREGGGFG
                     SIIGRNSFQRPKAEAIKFLNTIMDIYAGKIQ"
     gene            complement(3637..>4020)
                     /locus_tag="VLD60_05380"
     CDS             complement(3637..>4020)
                     /locus_tag="VLD60_05380"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HSB44421.1"
                     /translation="AINEGNYHKWPAGTRQYIDSLKKQDKSTGRPYSGRYSGCLVADV
                     HRILLGGGIYLYPGEIEKPEGKLRLLYEANPMALVVEQAGGRASTGLKRILDLEPKKL
                     HQRVPLIIGSRDDVAEAEEFIQQRR"
BASE COUNT          827 a         1265 c         1015 g          913 t
ORIGIN      
        1 acacgcgcga cctgcaactc ctctcgctcc gcctcttcct tgaggcgggt aaagatcatg
       61 cggccggctg tcgtctggag cacgctggtc accaccacat cgacattctt gccgatgtag
      121 cgcctcgcat tatccaccac aatcatggtg ccgtcgtcga gataggcgac gccttgaccc
      181 gcttccttcc cttctttcag cacaaagacg cgaatggttt cccctggcag caccacggga
      241 cgcaacgcat tgcagagttc gttgatgttc aggacccgca ctccctgcag ttcggccact
      301 ttattcaagt tcaggtcgtt ggtgacaatt ttggcattca gttttttcgc catcacgacc
      361 agtttggcat cgacttcttt cacgtgcggg aaatcttcat caatgatccg cacatccaca
      421 tcgcccatct tctgaatctt gttgaggata tcgagtccgc gtcggccccg cgcccgcttc
      481 aacgagtcgg atgagtcggc gatatgctgc aattcgtcga gaataaaatg agggaccaga
      541 aaggtccctt ccataaatcc ggtttcgcag agatccgcca cccgcccatc gatgatcacg
      601 ctggtatcga ggactttcgg acagaccgtg ttcccggtca actcgaaaaa cttttgctcg
      661 ccgctgggga acttttcctt cccgaaccgc gccccaaaca ccaacccgag gtacgggaac
      721 cccagtagaa agaccaaccc cccgatgtga aacaagaagg tttggacgtc gaatatttcg
      781 cccccaaccc actcgatcaa accggttaag agcaagccca ccgccagacc ggcggttccc
      841 cccacaataa tgccgaatga caggtttcgt aatgcatatt ccccggcgac aatgagccct
      901 cccgtcgatg ccccgatccc aaacccgtac aaccaataag acgcgtcagc gccttcggcc
      961 ttgatgaaca aggccatgcc ggctaacgca ctgagaagga cgaatatggc tctcgatacc
     1021 atacctttac ctcctccctc gattgccccg ctctcgcgag gcgatggagc gatccctacg
     1081 ggcgcaacgt cagatagatc gtctggccct ttcgtttgag cagcaggagg accgcctggt
     1141 ctttggtgag gcctgagact gcccgctcgt aggatttcaa cgagctcaca ggcttgcgat
     1201 tgacttccag aaccatgtcg ccttctcgta cgcccatttc ctcggccgtg ctgccgggtt
     1261 tcacccgaac gatgatgacc ccgcgttctg tcgtcttcaa gccatatcga gcggccagct
     1321 cttcattcag ctcgcggacg tcgagatccg acaatactcc agttggcgcg gtcgattcgc
     1381 cgccctcctc gacgctgggc tgcccgaacg acttcggttg ctccgcgatg gtgacctcga
     1441 gctgcttcgg cttcttgtcg cgaatcaact tcacggtaac ctttttcccg atcggcgttt
     1501 gcgcgacggc attccgcaaa tgtgtgggag aatccatcgg cttcccgtcg tattccgtaa
     1561 tcacatcggc gcgctcaaac ccggctttct tggccgggct ctcgtccata acatcgctga
     1621 ccagcacccc cttggtatcc gccagtccga actgcgtcgc cagttccggc gtcagttctt
     1681 gaatcgcaac gccaagccag ccccgaacca ctttccctgt cttcaccagc tgatccatga
     1741 tggtgcgggc cagattgctg ggaacggcga atcctattcc catgttcccg ccactttggc
     1801 tgaagatcgc cgtattgatc ccgacaagct cgccccgcac attgaccagg gcccccccgg
     1861 aattgccagg gttgatcgcg gcatcggttt gaataaagtc ttcatattcg gcaatgccca
     1921 tgctggctcg acccacggcg cttacgatgc ccatcgtgac gctctgcgtc aaaccaaagg
     1981 gactcccgac cgccaagacg aactcgccca cttcaagctg atcggaatca gccagcggga
     2041 tcgtggagag ccctgtcgcc tcgatcttca cgaccgcgat atccgtcttg gtatcggtgc
     2101 cgatcagttt gcccttgaac tctcgcttgt cggagagaat taccctgatc tcgtcggcct
     2161 tattcaccac gtgattgttg gtgataatca acccgttcgg atccacaatc acgcctgatc
     2221 cctgtccccg ttcttttcgc tcccgctgcg gagcgtcacg tttgaaaaat tcatcgccga
     2281 aaaacttgcg aaagaacgga tcgtcaaacg gcgaggattg cgggccttct cccgctttac
     2341 cgcttctggt cgcggcaata ttgacgacgg cgggcttgac catcttggca atttcgacga
     2401 agtttttcgc gttccctccc cccatcggca tctgcggcgc tgtggcaacg ggacggacga
     2461 tgccgttcgg gatgggatcc ggtccggccg tgccggtcgg caaccaaccc atgtcggacg
     2521 ccacgacgac gccgatgaca atcccgacag tgatgaggat gaacggaacg agccaagaac
     2581 gaccgtgctt catacgtgac ccttactgaa tttttccggc gtaaatatcc atgatcgtat
     2641 tcaggaactt gatggcttcg gccttgggtc gctggaacga atttcggccg atgatcgagc
     2701 cgaatcctcc gccttctcgg atcgcccgcg cttcttcgaa cacattctta tcttcactct
     2761 tggcgcctcc ggaaaatatg acgatccgct gtccatcgaa ggcactctgc acgacatgct
     2821 tgacccgttc ggccagcgtc ttaatcggaa tctgttcggt ttcatagact ttcttggcgg
     2881 cggcctgttc gaggtgcgcg gacggaagct tgaccttaat gatatgcgcg ccgagttgag
     2941 cggcaatctg cgcggcatag gccacgacgt ccatcgcggt ctccccttcc ttactcaatg
     3001 ccgacccacg gggataggac cagaccacga ccgccaatcc gtgatccttg gcctcctccg
     3061 cgatggcacg gacctgctca tacatggcgt tgcaatggga ggagccggga taaatcgtaa
     3121 accccacggc ggagcagcct aagcgaagcg catccttgac gcttccggtc accgaaggca
     3181 gcggatcctt ctcatcgtgg aggacatcat gattgttcaa cttcaagatg aggggtattt
     3241 gtccagcata gtggctggca ccggcttcaa ggaaccccag cggcgccgca taggcattgc
     3301 aaccggcatc gatggccaat tgaaaatgat agagcggatt atagccgggt ggattgggcg
     3361 caaaactccg cgcgggtcca tgttcgaatc cctggtcaac cgggaggatc accagtttgc
     3421 ccgttccggc taatctaccg ctccgcaaca accggccgat gttggctttc gttcccgcat
     3481 tgtcgctacc gtatccactt aagatctctt gcacgcgatc agccatgctc aacctcctgt
     3541 agattcgtga tacgtgaaac gttattcgtt aaacgtcaat cgtcgagaac ctgggccatc
     3601 caaaatttgt ctcgtttcac gtgtaacgat tcacgattaa cgcctttgtt ggatgaactc
     3661 ctccgcttcg gcgacgtcat cccgacttcc aatgatcaac ggtactcgct gatgcaactt
     3721 cttgggttcg agatccagaa tccgcttcaa gccggtactg gcccggccac cggcctgttc
     3781 gacgaccagg gccatcggat tggcttcata gagcaaacga agcttacctt ccggcttttc
     3841 gatttcacct ggatacagat agatcccgcc gccgaggaga atccggtgga catcagcaac
     3901 aagacaaccg gagtaccggc cgctataggg acgccctgtc gatttatcct gtttcttgag
     3961 ggagtcgatg tactgccgcg tcccggccgg ccacttatgg tagttcccct cgttgatcgc
//
LOCUS       DATIFR010000097        17787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2738965, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000097 DATIFR010000000
VERSION     DATIFR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738965"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="VLD60_05385"
     CDS             complement(<1..1808)
                     /locus_tag="VLD60_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB44422.1"
                     /translation="MPTVLLLTPPLTQLNTPYPATAYLTGFLRKHHIPVSQADIGIEM
                     VLKLFSHPGLASVFAEVRMRRDDLPPEARAMLEAEQSYMTTIDTVIAYLQSRDPALAP
                     RIVSPGFLPRGPRFHRRTRKQPPEQDRATQFATFYLEDLADLVQAVVSPHFALSRYAE
                     HIARAASSFDRILDALNAEPTLTDRFMLEALWQHIKRAQPSLIGLTVPFPGNLYGAFR
                     LAQAIKAQRPDIVVALGGGYANTELRRVSDQRVFDYVDFITLDDGERPLLSLIEHLAG
                     TRPKTKLTRTFHREKDHVVYTNDTSDRDFSMDEVGCPTYSGLDLNRYLTILDSTNPMH
                     RLWSEGHWNKLTVAHGCYWKQCTFCDVGLDYIGRYEMTPTVRLIRQIEQLIAETGRRG
                     FHFVDEAAPPAALKALALGLLERGIDITWWGNIRFEEAFSPDLCQLLAASGCIAVTAG
                     LEAASDRILEKIKKGITVDNTALVSAAFNDAGILIHAYLMYGCPSETIQETIDSLERV
                     RQLFAAGLMQSAFWHRFTATAHSPVGLQPARHGIRILGPAFEGFAENDLTHEDRFGQT
                     PNWLGEGLRRSMLNFLEGRGLTLDVRQWFDCPAPKP"
     gene            1938..2495
                     /locus_tag="VLD60_05390"
     CDS             1938..2495
                     /locus_tag="VLD60_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSB44423.1"
                     /translation="MSDSYILYSDFNFPFCYALHERLYDMDLLGHCEWRGVQHAPHLP
                     RPMKPWEGPLGAELRQEVAVVRLVAPGLPIALPSGKPNTKPAIEQAVQWLRRDRSKGM
                     EYVRQIYRALWCDGRDISDPSVLAHLAMAPSVGEVDEESRRIVREWEAAWNATGQAGV
                     PLIVSPAGDLLIGSVPVEEIQRFFV"
     gene            complement(2563..3456)
                     /locus_tag="VLD60_05395"
     CDS             complement(2563..3456)
                     /locus_tag="VLD60_05395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB44424.1"
                     /translation="MTEDIRIGFVGVGRMGANMARRLKDKGYHVTAVYDQQAAASKSL
                     AVELQCEDAKTPARVAELSDVVLTAVSDDAAMLEIYEQGNATTLLTHAKGRLFLNCAT
                     VSPQIHIDVETLVERYGGLSLEACMASSITQARQGTLYLICGGKQTAFDRAKPILQSL
                     SSNLRYVGKSGEAAKVKALVNMVMNCNTAALAEGLGLGEALGLDLTMLREVFSQTGAA
                     SRVLETDGEDMQLRAHDCYFSAAHAAKDSGIALALAKSAGLRLPLAQATFDQYQRLVA
                     AEKGELDKSGIAELTFKDRMG"
     gene            3621..4028
                     /locus_tag="VLD60_05400"
     CDS             3621..4028
                     /locus_tag="VLD60_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSB44425.1"
                     /translation="MQDNDAATIDEIFRICRTIAVVGASSNPARPSNRVAGYMKAQGY
                     RMIPVNPKEQTILGEQVYPSLTSVPDAIDLVDVFRKAEEVLLIVEEAITKGAKAIWLQ
                     EGIVNEAAAVRAKQAGLLIVMDRCLLKEYRRRL"
     gene            complement(4038..4457)
                     /gene="msrB"
                     /locus_tag="VLD60_05405"
     CDS             complement(4038..4457)
                     /gene="msrB"
                     /locus_tag="VLD60_05405"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSB44426.1"
                     /translation="MPQNINIGPIVKVVKTDEEWKKLLSPEAYRVLRHEDTERPFTNN
                     MHDNKKAGIYYCAGCDLPLFSSEHKFDSGTGWPSFWQPIDPKVVETTTDYKLIFPRTE
                     VHCARCEGHQGHIFKDGPKPTGLRYCINGAALKFVAA"
     gene            complement(4601..6991)
                     /gene="lon"
                     /locus_tag="VLD60_05410"
     CDS             complement(4601..6991)
                     /gene="lon"
                     /locus_tag="VLD60_05410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSB44427.1"
                     /translation="MNTETRTLTLPVLPIKRTVLFPGIMMPLTVGRERSVAAVNAAMQ
                     TEDKTILVVAQRDPLTEEPGLNDLYTIGTKATVKQLGRSDDGNIHALVQGLERVALLK
                     VEQAKPYILAQARRLDPPTNVDTEAKALHRAIQELVTDLPRLIQAPGIQEAAAAFNNE
                     DDPVALAYRVASLLNLTVQEEQKLLEHSSATELLRALYAALSKEIQILQLRNKIASEA
                     QANIGKNQREYVLREQLKAIQQELGEGEDEENEVARLKKQIQEADLPDHVRKEAEREI
                     TRLGKVPPSSPDHQVLRTYLELVLELPWKKASEEHLDLAKVRQVLEEDHYGIKEVKER
                     IVEHLAVLKLNPTAKAPILCLVGPPGVGKTSLGQSIARAMGRTFERFSLGGVHDEAEL
                     RGHRRTYVGALPGRIIQAMRRANVNNPVLMLDEVDKLGRDFRGDPAAALLEILDPAQN
                     HTFRDHYLDLPFDLSKVFFITTANTLDTISQPLLDRMEIIRVQGYSEREKAEIARRYL
                     WPRRLKEAGLDASQALLSDEVLNLVISRYTREAGVRQLEQMLGRLTRKVALAFAELPE
                     GQERTAATIQPAQLSEWLGSERFMPEEARKHLPPGVATGLAWTPTGGDVLYIETTLLP
                     GSHELTLTGQLGDVMQESARAARSYLWSHAEGMGLDISRFKRNGVHIHVPSGAIPKDG
                     PSAGITMATALASSYEGKAVRSDTAMTGEISLSGLVLPVGGIKEKMLAAHRAGIRRVI
                     LPKANEKDLKDVPQEVRDDMTFIPVERIEEVLPAAFDHDRAAHGASAPEQPLSASA"
     gene            complement(7145..9106)
                     /locus_tag="VLD60_05415"
     CDS             complement(7145..9106)
                     /locus_tag="VLD60_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44428.1"
                     /translation="MVQGFFSFLGPPATATPTSRMRESALERVTPTVRHDLDRLVPVA
                     QSAFIAAHETVETVPARFKRRLVVDSLKQPWEGLPFLETQGLLVAELAEGGAVSLPGL
                     LDILEAGVDRTSAFHKSIPLPSKLTAQELATFMLETLELASRHREKAVENLTEEEGRF
                     LFLHGKNLVEQFTPQISTSSDQAIARAKADTRFTELIEEHVDYANLIAAAQVLARLAN
                     VPWQQYVHIVFPRTVPSVQTPAGITGDILYAEETPYGLIVIGGPGPNTYELDNRFGLV
                     IDVGGDDLYRGMIAASTDPEHGNATVIDVSGNDTYQGAVLGLATGRLGVGLLIDQSGD
                     DVYQLDMGSGGAGLGGLGILFDAKGNDVYMGSRFTQGAAVGGLGLLFDGGGNDRYTSH
                     GFSIGFGGPLGVGAVLDVQGDDQYQCGNKYPSAYNAEDAPNGKPGDPLFQYDCFGLGT
                     GSGKRMLTKRPEGLVHSLAGGWGLLLDIEGHDDYQSANFSQGHGYFFGAGMLLDLDGD
                     DEYHAARYGQGSSAHYGVALFTDRHGDDRYSSTGPFYNSGVSWDYGVSLFLESGNGKD
                     QYDLERSTGLGGADLSGWGLFIEEGGNDQYRVKSGFGRSSEQGLAAFFDLNGKDTFIL
                     PQNSTGPVDESPRNNRVLRYPKGGLFVDR"
     gene            complement(9194..10372)
                     /locus_tag="VLD60_05420"
     CDS             complement(9194..10372)
                     /locus_tag="VLD60_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSB44429.1"
                     /translation="MDSTKHRFFAPCPRGLESVLEDELHGLGVPATTKTDGGIAFHAP
                     WSSMYWVNLNSRIASRVLWEVGHSPYRSEEDVYRAAYSLAWPDWFLPSQTIKVKVSAR
                     HCPLKSLDFVTLRIKDAVCDKFVSVRHKRPPVDTQRPNIKIDAFLDATTVTFYLDTSG
                     EPLFKRGHRIATVDAPLRENLAAGLIKLSGWTPTDVLLDPMCGGGTIPLEAALMARHI
                     APGFSRTFAFERLLLHDAKRWGNLREVARLKQKPETPVAIYASDQDPAAIKIAQRNFQ
                     GAGVAIDIRLRQSNILALEAPTEQGIMLINPPYGVRLSQPDELEFFYPRLGDWLKQRF
                     AGWRVYVFTGDARVPKLIGLAPTKRTPLFNGALECRLYEFIIVQGSMRKNRSSVHGNP
                     "
     gene            10405..10944
                     /locus_tag="VLD60_05425"
     CDS             10405..10944
                     /locus_tag="VLD60_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSB44430.1"
                     /translation="MNYCSECGKPVSKKIPPGDNLPRFVCSTCQSIHYHNPKIVAGCI
                     PEWEDQILLCRRAIEPKSGLWTFPAGFMEIGEGIEQAARRETMEEAQAQVAGIRLHSV
                     LSLPHIGQVYMTFVGALLAKQYGAGSESLDVQLFERAKIPWGEIAFPVVKEALRRYLE
                     DVAHGQFRLHVADIPDRSV"
     gene            complement(10974..11237)
                     /locus_tag="VLD60_05430"
     CDS             complement(10974..11237)
                     /locus_tag="VLD60_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSB44431.1"
                     /translation="MAWYRLTFQPAVVQDLAAIGTPMAQRLFDKTKWIASNVENLRHE
                     NVDAALPGIHKYAVGEWRLFYAIDQVEHLLEIHAIVHRNQLRT"
     gene            complement(11244..11447)
                     /locus_tag="VLD60_05435"
     CDS             complement(11244..11447)
                     /locus_tag="VLD60_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44432.1"
                     /translation="MDLASLSPEQLKELVRGLVDDRLRELIGDPDLGLEVGEALRTRL
                     KSSLANNARLSGEEIAEKLGLHW"
     gene            complement(11499..11825)
                     /locus_tag="VLD60_05440"
     CDS             complement(11499..11825)
                     /locus_tag="VLD60_05440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB44433.1"
                     /translation="MVTRKHRRFAVTMPSTLVQRDQFRHNGSIRDISARGCRVESMIS
                     PFTGMQVTLLLHVPGEPNPIMIENAAVRWCGAQGIGVEFLIVAQPEQDRLGRFIKRLE
                     AAAPSN"
     gene            complement(11847..12170)
                     /locus_tag="VLD60_05445"
     CDS             complement(11847..12170)
                     /locus_tag="VLD60_05445"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB44434.1"
                     /translation="MGVKDQREHDRYLVEFPATFAGECAGVGIVYNLGMGGCKIVTDC
                     PPLVGAMVAIYLKVPEQTFAITIRMATVRWTMEYEFGAEFLGMEELERDRLTKFLEGL
                     EAAAA"
     gene            12329..13048
                     /locus_tag="VLD60_05450"
     CDS             12329..13048
                     /locus_tag="VLD60_05450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB44435.1"
                     /translation="MKRLEDKVAIVTGSSSGIGKAIALRFGEEGAKVVVAARRKSLCE
                     EVARTICQKGGDAFVIQTDVSHEQQVDALFEKTIQQYGRVDIVVNNAGIFGGRRIAET
                     TTKAFDDVMSVNLRGTFFCCRAAFKHMKKQGGGVILNMSSVAGVQAWAGTGAYSASKH
                     GVMALSRSLAEEGRPFGIRVSAICPGGVANDLVDASQKEILRSEKISPFDIAETAIYL
                     ATLGKYAVVHQIVVDRWAADW"
     gene            complement(13070..13546)
                     /locus_tag="VLD60_05455"
     CDS             complement(13070..13546)
                     /locus_tag="VLD60_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44436.1"
                     /translation="MTGMEASPLRSHHHWLLAAVCLSATALTATPALANKAQVPWECS
                     NYSDEAQTRCLNAFIEQQREQIGKLEGQLRAQQEAVGQLKGQIDRQAAATAGIQQQLS
                     QRPTTVVVPGPYAFTYVYPPVGLGFYLGSPWIYGPPSFYGRVYWGPRYHGRWGRHW"
     gene            13799..14452
                     /locus_tag="VLD60_05460"
     CDS             13799..14452
                     /locus_tag="VLD60_05460"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSB44437.1"
                     /translation="MKRRFQRRLLAWYEKNGRDLPWRRTADPYHILVSEVMLQQTQVD
                     RVVPKYHEFLERYPSFEDLADAPVTEVKQTWYPLGYNIRPERLHGIACETVARYGGKL
                     PSDHEELLSFKGIGRYTAGAIRSFAFNEDAPILDTNVMRVLHRVFVAKGDPKTRKAGL
                     WELSEHLIPRGKGYDFNQALMDFGATCCTARNPECSRCPMKSFCRSYPFNPKPRARS"
     gene            14449..14847
                     /locus_tag="VLD60_05465"
     CDS             14449..14847
                     /locus_tag="VLD60_05465"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HSB44438.1"
                     /translation="MKVVEVAAGLIRHKGRYLIAKRKPGVHLAGFWEFPGGKREPGET
                     LEECLQRELFEELNIRIDTPIPFRIIRHDYAEKAVELHFFHCRIEKGFASPLDCAEIR
                     WVYPHELAGFQFPPADRPIIDALQREGVNS"
     gene            14844..15560
                     /locus_tag="VLD60_05470"
     CDS             14844..15560
                     /locus_tag="VLD60_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSB44439.1"
                     /translation="MKVVLDIETIQAPREEWERLVGKPQTKGDADPSGEGFDLFAAGA
                     AEEERRAEDEQYAKSAFDGTFSSIVCIGLLEFSDQMEPRGAVAWYGGNERELLRQFWS
                     RLAQDRPSLFITHNGLGFDLPFIKKRSIIQQVKPSLEINLAKFRTEPVYDTMAIWSNW
                     DTRGWVKLDVLARALNVETKSGSGEQVAAMWAKGHGLELARYCLQDTYVTYACYCRMS
                     FRQPLSREVVLLQPELLEVG"
     gene            complement(15567..16454)
                     /locus_tag="VLD60_05475"
     CDS             complement(15567..16454)
                     /locus_tag="VLD60_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44440.1"
                     /translation="MPTRSFPTKTLLMHVCLGAALVMSGCSSTSKIAQNAKGSVYLEE
                     VMDWSFEASHPAVIDQLTIMKVVKGVYGDGSSRMSAGGSKPMRVFSDEDAEFLAPLLA
                     QGLSKAKPEQLVGFRVSSSAGSGSEPTAGSIYVRQGLIHFTISKGAGSTTFAPESAAR
                     TEYAPSYAAGGMGGVTAHVIDLQALAKAPMPAAMPVAKTSQKTATILTADSAKTQQAP
                     TASASLVDENQFQQREIDQLSKAKETIAKKDTEISVLRKESEWMKRELRERDEELKAL
                     KASVKQAPKKKRAEARPTR"
     gene            complement(16533..16670)
                     /locus_tag="VLD60_05480"
     CDS             complement(16533..16670)
                     /locus_tag="VLD60_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44441.1"
                     /translation="MNSASNQPQNTEKESSTSVSLETIIAMGLFGWLALSMTLMGLGI
                     W"
     gene            complement(17086..>17787)
                     /locus_tag="VLD60_05485"
     CDS             complement(17086..>17787)
                     /locus_tag="VLD60_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB44442.1"
                     /translation="YRFGEWDFARLLTAVADVPGIERVRFTSPHPKDFPPALLEAVAT
                     HPHICKHIHLPLQSGNDRILEMMNRTYTRKEYLALVDQIRAMNPDIALTTDVIAGFCS
                     ETDAEFSDTYALLEEVRFHSAYVFLYSERKNTIAARKFADEIPDQVKSKRVTALVELQ
                     RRISTERNREYLGTTLRVLVEGDAKKSHLQAMGKTDGNITVVWEKAAAPCSPGQILSL
                     EVYDASASTLYARPA"
BASE COUNT         3758 a         4749 c         5312 g         3968 t
ORIGIN      
        1 tgaggtttcg gcgccggaca atcgaaccat tgccgcacat ccaacgtcag gccccgccct
       61 tcgagaaaat tcagcatcga ccggcggaga ccttcaccga gccagttcgg ggtttgacca
      121 aagcgatcct cgtgagtcaa atcgttttca gcgaacccct caaatgccgg tccgagaatg
      181 cggatgccgt gacgtgccgg ctggagcccg accggactgt gcgccgtggc agtaaagcga
      241 tgccaaaagg cggattgcat caggcctgcc gcaaacaatt gccgcactcg ctcgagcgaa
      301 tcgatggttt cctgaatggt ctcggaaggg catccataca tgaggtaggc gtgaatcaaa
      361 attccggcat cgttgaaggc tgccgacaca agtgcggtat tgtcgacggt gatgcctttc
      421 ttgatcttct ccaggattct gtcggaggcg gcttccagtc cggccgtgac ggcaatgcat
      481 cctgaggcag cgaggagctg gcacaggtca ggcgaaaagg cttcttcgaa ccggatgttg
      541 ccccaccatg taatgtcgat ccctcgctcc agcaggccga gcgccagcgc tttcaaggct
      601 gcgggcggcg cagcttcgtc gacgaagtgg aatccccgcc ggccggtttc ggcgatcaac
      661 tgctcgatct gtcggatcaa tcgaacggtc ggcgtcatct catagcggcc gatgtaatcc
      721 agccccacat cgcagaacgt acattgcttc cagtagcagc cgtgggccac ggtcagtttg
      781 ttccagtgcc cttcggacca gagacgatgc atggggttgg tgctgtcgag gatcgtcaga
      841 tagcgattca agtccaagcc gctataagtc gggcagccga cttcgtccat actgaaatcc
      901 ctatcggatg tatcgttcgt gtagaccacg tgatccttct cacgatggaa ggttctggtc
      961 aatttggttt tgggtctggt gccagccaga tgctcaatca aggaaagtag gggccgctcg
     1021 ccatcgtcga gtgtgatgaa atccacatag tcgaacacgc gctggtctga tacgcgccgc
     1081 aattcggtat tggcgtagcc cccgccgagc gcgacgacga tatccggtcg ctgagccttg
     1141 atggcctggg caaggcgaaa cgcgccgtag agattgccgg ggaacggaac ggtgaggccg
     1201 atcagagagg gttgtgcccg cttgatgtgt tgccacagtg cttcgagcat gaatcgatcc
     1261 gtcaatgtcg gctcggcgtt cagtgcatcc aggatccggt cgaaggacga ggcggctcgc
     1321 gcgatgtgct cggcatagcg gctcagggcg aaatggggcg acacgacagc ctggaccaag
     1381 tcagccaggt cttcgaggta gaacgtcgcg aattgtgtgg cgcggtcttg ttccggcggc
     1441 tgtttacgcg tgcgtcggtg gaagcgcgga ccgcggggaa gaaaaccggg agaaacaata
     1501 cgcggcgcca acgccgggtc gcgtgattga agatatgcga tgaccgtatc aatggtagtc
     1561 atgtacgact gttcggcttc gagcatcgct ctggcttcgg gcggcagatc atctctacgc
     1621 atccgtacct cggcaaagac ggaggcaagt cccgggtgac tgaacagttt gaggaccatc
     1681 tcaatgccga tgtccgcttg cgagactggt atgtgatgct tgcggagaaa gcctgtgaga
     1741 taggccgtgg cagggtaggg cgtattcagc tgggtcaagg gcggtgtgag cagaagaacg
     1801 gtcggcatcg gcatgcgctc acataacata ccggtttgag ggattgccag gctggttatg
     1861 tcatcgatag cagaagacta ttggaatatc ttccgagacc tgcgctaccg tgatgtcttg
     1921 cgaggagagg gattcctgtg agtgattcct acattcttta cagcgatttc aactttccgt
     1981 tctgttatgc tctgcatgaa cggctgtacg acatggatct acttgggcat tgcgaatggc
     2041 gcggcgtgca acatgcgccg catctgcctc ggccgatgaa gccctgggag ggaccgctgg
     2101 gcgctgagtt gcgacaggag gttgcagtgg tgcgactggt agcgcccggt cttccaattg
     2161 cgctgccgtc gggcaagccc aatacgaaac cggcgattga acaggctgtc caatggctgc
     2221 ggcgggatcg cagtaagggg atggagtatg ttcggcagat ctatcgagcg ttgtggtgtg
     2281 acggacgaga catctcagat ccgtctgtat tggcacattt ggcgatggcg ccatcggtcg
     2341 gcgaagttga tgaagagagt cgtcgcatcg tccgggaatg ggaagcggcc tggaatgcga
     2401 cgggccaggc cggggttcct ttgattgttt cgccggccgg cgatctgctc attggctctg
     2461 tgccggttga agagatccag cggttctttg tttgagtttc acgctgtgcc ttgaagtaga
     2521 cgaagacgat cccggcatat cgccgtttcg ttcgacgggg cactatccca ttcgatcctt
     2581 aaacgtcagt tcggcgatgc ctgacttgtc caactcgcct ttctcagcag ccacgagccg
     2641 ttggtactgg tcaaaggtgg cctgggcgag gggcaatcgg agcccggcgg atttggcgag
     2701 ggcgagagcg atgcctgaat ccttcgctgc atgggcggcg gagaagtagc agtcgtgggc
     2761 tcgaagctgc atatcctcgc catccgtctc cagcacgcgt gaggccgcgc cggtttggct
     2821 gaagacttca cgtagcatcg tgagatccag cccaagcgcc tcaccgaggc ccaagccctc
     2881 cgcaagcgcg gccgtgttgc aattcatcac catgttgacc agcgctttca ctttggctgc
     2941 ctcgccgctt tttcccacat agcgcaggtt actgctcaga gactgaagga tcggcttggc
     3001 ccgatcgaac gcggtttgtt ttcctccgca gatgagataa agcgtgcctt gacgcgcctg
     3061 ggtgatgctg ctggccatgc aggcttcgag gctcaaaccg ccatatcgtt ccaccaacgt
     3121 ttcgacatcg atgtgtatct gagggcttac cgtcgcgcag ttgaggaaga ggcggccttt
     3181 cgcatgggtc agcagggtcg tggcgtttcc ttgttcgtag atctcgagca tcgcggcatc
     3241 gtccgatacc gcggtgagga ccacatcgga taattcagct actcgcgcag gagtctttgc
     3301 gtcttcacac tgtaactcga cggcaaggct cttggaggca gcggcttgct gatcgtagac
     3361 cgctgtgaca tggtaaccct tgtctttcaa gcgcctggcc atgttggcgc ccatgcgacc
     3421 gacaccgacg aagcctatgc ggatgtcctc tgtcatggga cgactccttc acgtttgcgg
     3481 gacggctgac acgcacagaa ttaccttgtg gtggtatgcc tgatgccgtc gacaccgtca
     3541 agagaacagt ccggtctgat ggaattgtgc ggattcgcgg gtacatggta gggttcggtc
     3601 ggcggaagga gtctgaactc atgcaggaca atgacgctgc gaccatcgat gaaattttcc
     3661 gtatctgtcg aaccatcgcc gtcgtggggg catcatccaa tcccgcgcgt ccctcgaatc
     3721 gagtggccgg ctacatgaag gcccagggct atcggatgat tcccgtcaat ccgaaggaac
     3781 agaccatact tggcgaacag gtctacccct cgctgacgtc ggtacctgac gcgatagatc
     3841 tcgtggacgt gtttcgtaag gccgaggagg tcttgctcat tgtcgaagag gccattacga
     3901 aaggcgcgaa ggctatttgg ttgcaggagg gaattgtgaa cgaggccgcc gctgttcgcg
     3961 ccaagcaagc aggtctgctg atcgtgatgg atcgctgctt gttgaaagaa taccgccgtc
     4021 gactgtaacg acacggatta cgccgccaca aactttagcg ccgcaccgtt gatgcagtat
     4081 cgcaggccgg ttggtttcgg tccgtccttg aagatatggc cttggtggcc ttcgcagcgg
     4141 gcacaatgga cttccgtcct ggggaagatg agtttgtagt ctgtagtcgt ctcgactact
     4201 ttcgggtcga tcggctgcca gaagcttggc cagcctgttc cgctgtcgaa cttgtgctca
     4261 gaagaaaaca ggggcaggtc gcagcctgcg cagtagtaga taccggcttt cttattgtcg
     4321 tgcatgttat tggtaaaggg ccgttcggta tcttcgtgcc gcaagacccg gtaggcctcc
     4381 gggcttaaga gcttcttcca ttcttcgtcg gtttttacca ctttaacaat cggaccgatg
     4441 ttaatatttt gcggcatggg tttgctcctt tccagcggga cttccccaac gtcagtcggg
     4501 aagccggagt catccttaca taccggtcgg cagatgggca agacgagggg gacgaaagaa
     4561 ggggagggac tccggaggtt ggttccggag tccctggatt ttaggctgac gcagacaaag
     4621 gctgctcagg ggctgaggct ccatgagcgg ctcggtcgtg atcaaaggca gcgggaagaa
     4681 cttcctcgat ccgctcgacc ggaatgaagg tcatatcgtc ccgcacttcc tgcggcacat
     4741 ccttcaaatc cttctcgttg gcttttggca ggatcacccg cctgataccg gcgcgatgag
     4801 ccgccagcat tttctctttg atccctccga ccggaaggac caacccgctc agactgatct
     4861 cgccggtcat cgccgtatcg ctgcggacgg cttttccttc gtacgaggaa gccagcgcgg
     4921 tggccatcgt gatcccggct gaggggccgt ccttcgggat ggcaccggac ggcacatgga
     4981 tatgcactcc gttacgtttg aagcgggaga tatcgagtcc catgccttcg gcgtgtgacc
     5041 agagatagct tcgcgcggcg cgcgcagact cctgcatcac atcgcccagc tggcctgtga
     5101 gggtcagttc atggcttccc ggcagcagag tcgtttcgat atagaggaca tctcctccgg
     5161 tcggagtcca ggccaaaccg gtcgcaacgc ctggaggcaa atgtttacga gcctcttccg
     5221 gcatgaaccg ctccgacccg agccattccg acaactgggc cggttgaatg gtcgccgcgg
     5281 tgcgttcctg gccctctgga agttccgcga atgccaaggc cacctttctc gtgaggcgac
     5341 cgagcatctg ctccagctgg cgcacgcccg cttcccttgt atagcggctg atcacgaggt
     5401 tcaagacctc gtcagagagc agtgcttggc tggcatccag gcccgcttcc ttgagccgtc
     5461 ttggccagag atagcgacgg gcgatctcgg ctttttcccg ctcgctgtac ccctgcaccc
     5521 ggatgatttc cattcgatcg agcaagggtt ggctgatcgt gtccagcgtg ttcgcggtcg
     5581 taatgaagaa taccttcgac agatcgaagg ggagatccag ataatggtcg cggaatgtgt
     5641 gattctgcgc tggatccagg atctccaata gcgctgccgc tggatcgcca cggaaatcgc
     5701 gtcccagctt atcgacctcg tccaacatca gcacgggatt gttgacattg gctcgacgca
     5761 tggcctgaat gattcggccg gggagcgcac cgacataggt tcggcgatgg ccgcgcaact
     5821 ccgcctcgtc atgcactccg cccaggctga accgttcgaa ggtccttccc atggctctgg
     5881 caatcgattg gcccagactc gttttcccca cgccaggcgg tcccactaag cagaggatcg
     5941 gcgccttcgc cgtgggattc agtttcagaa cggcgagatg ctccacgatc cgttctttca
     6001 cctctttgat gccgtaatgg tcttcttcca gcacctggcg aactttggcg aggtcgaggt
     6061 gctcctcgga ggctttcttc catggcagtt ccagaaccag ttccagatag gttctgagta
     6121 cctggtgatc cggcgatgaa ggcggaacct ttccaagacg cgtgatttct cgttcggctt
     6181 ctttgcgcac atggtcgggc aggtcggctt cctgaatctg cttcttgagg cgagcgacct
     6241 cgttctcttc gtcctcgcct tcgccgagct cctgctggat cgccttgagt tgttcgcgaa
     6301 ggacatactc acgttggttc ttgccgatat tggcctgcgc ctcgctggca atcttattgc
     6361 gaagctgaag aatctgaatc tctttcgaga gcgccgcgta caatgcccgc agaagttccg
     6421 tcgcggagga atgttcgagc agcttttgct cctcctggac ggtgagattg agcagagagg
     6481 cgacgcgata ggccagtgcg acagggtcgt cctcattgtt gaaggctgcg gccgcttctt
     6541 gaatgcccgg tgcctgaatc agccgcggaa ggtctgtaac cagttcctgg atggcccggt
     6601 gaagagcctt ggcctctgtg tcgacattcg tggggggatc gagtcggcgg gcctgtgcga
     6661 gaatataggg cttggcttgc tccaccttga gcaaggcaac ccgttcgagt ccctgaacga
     6721 gggcgtggat gttcccatcg tctgaacgtc ctagctgttt gacggtggct ttcgtgccga
     6781 tggtatagag gtcgttgagt cccggctctt cggtcaaagg atcacgttgg gccacgacga
     6841 ggatcgtctt gtcctccgtc tgcatcgcag cattcactgc ggcgaccgag cgttcgcggc
     6901 cgaccgtcag gggcatcatg atgccgggga agagcacggt gcgtttaatc ggtaggaccg
     6961 gcaatgtgag agttcttgtc tcggtattca tgtcagcatc ccttcgctcg gtcggtaatc
     7021 agacggtaca tgcgtgagtc gtgaagaagg agataggttc gaccaagatc aagtcaaggg
     7081 gaggacgcaa cgcgactgga ttaaccgttc ctaatcatgg agatgtctga ctgcaagacg
     7141 ttgttcaccg atcgacaaat agtccgccct tcgggtatcg cagcacgcgg ttattgcgag
     7201 gtgattcgtc gaccggaccg gtcgaattct gcggcagaat gaaggtgtcc tttccattga
     7261 ggtcaaaaaa tgcggcaagg ccctgctccg atgagcggcc aaatccggac ttgacccggt
     7321 actgatcgtt acctccttct tcaatgaaga gaccccagcc agaaagatca gcgccgccca
     7381 aacccgtcga acgctcaagg tcgtactgat ccttgccgtt gccgctctcg aggaaaagac
     7441 tcactccata gtcccaggac acgccgctgt tgtagaaagg cccggttgag ctgtagcgat
     7501 catcgccgtg acggtcagtg aacagcgcga ccccgtagtg tgcggatgaa ccttgtccat
     7561 agcgagcggc gtgatactcg tcgtcaccgt cgagatcgag gagcatgccg gctccgaaga
     7621 agtagccgtg gccctgcgag aaattggcac tttgatagtc gtcgtgtcct tcaatatcga
     7681 gtagcagccc ccatcctccg gcgaggctgt ggacgagccc ctccggtcgt ttggtcagca
     7741 tacgtttccc tgaaccggtg cccaacccga aacagtcgta ctggaagagg gggtcaccgg
     7801 gcttaccgtt cggcgcgtct tcagcattgt aggcactcgg gtacttgttg ccgcattggt
     7861 attgatcgtc tccttgcaca tcaaggacag cgccgactcc taatgggcca ccaaagccga
     7921 tggagaaccc gtggctcgtg taccggtcgt tgccgccccc atcgaacagg agaccgaggc
     7981 ctcctactgc ggcgccctgc gtgaatcgac ttcccatgta gacatcattg cctttggcat
     8041 caaagagtat cccgagtcct ccgagtcctg ctcctccgga tcccatgtcg agttgataga
     8101 cgtcgtcgcc tgactggtcg ataaggagcc cgacgccgag gcgtcccgtg gcaagcccca
     8161 ggactgcacc ttggtacgtg tcgtttccgc tcacgtcaat caccgtcgca ttgccatgct
     8221 ccggatcagt ggaggcggcg atcatgcccc gatagagatc gtcgcctccc acatcgatca
     8281 cgagtccaaa gcgattgtcc aactcgtagg tattgggccc cggcccgccg atgacgatca
     8341 ggccgtaggg agtctcttcg gcatagagaa tatcgccggt gattccggca ggagtttgca
     8401 ctgaaggcac agttcgggga aacacgatgt gtacatactg ttgccacggg acattggcca
     8461 atctggctag aacctgcgct gcggcaatga gattcgcata atcgacatgt tcctcgatga
     8521 gttcagtaaa tcgcgtatcg gctttggccc gggcgatggc ctggtccgag gaggtcgaga
     8581 tctgaggagt aaactgttcg acgaggttct tcccgtgaag aaacaggaag cgtccctcct
     8641 cttccgtgag attctccacg gccttttccc gatgccgcga agcgagctcc agtgtttcca
     8701 acatgaaggt ggcaagctct tgagcggtga gtttggatgg cagagggatc gatttatgaa
     8761 acgccgaggt ccggtcaacc cccgcctcga gaatatcgag gagaccgggc aaactcaccg
     8821 ctcctccttc tgccagctct gccacgagca atccttgcgt ttccagaaag ggcaaacctt
     8881 cccatggctg cttcagcgaa tcgacgacga ggcgacgttt gaatcgagcc gggaccgttt
     8941 ctactgtttc atgggctgcg atgaaagcag actgagcaac agggacgagg cgatcgaggt
     9001 catgccgaac cgtcggggtc acacgctcca aagccgattc gcgcatgcgg ctggtcggcg
     9061 tggcagttgc cggggggcca agaaatgaaa agaagccttg aaccatgcta cagccgggca
     9121 aggagacaag gaacacaatt gtcactggca ggagccatcg gctagtcatg gtcatggaga
     9181 atagcgtgga cggttatgga ttgccatgga cagaagaacg attcttccgc atcgacccct
     9241 gcacgatgat gaactcatag agccggcatt ccagtgctcc attgaaaaga ggtgtgcgtt
     9301 tggtcggagc cagaccgatc aacttgggaa cgcgagcatc tcccgtaaaa acgtacacgc
     9361 gccatcctgc aaaacgttgt ttgagccaat caccgagcct gggatagaaa aattccaact
     9421 catccggctg actcaggcga acaccgtaag gggggttgat cagcatgatg ccctgttcgg
     9481 taggggcttc aagagcaagg atattgcttt gacggagtcg aatgtcgatg gccacgccgg
     9541 ctccttgaaa gtttcgctgg gcgatcttga tggccgcggg atcttgatcg gaagcataga
     9601 tggcgacagg agtttcgggt ttttgcttga ggcgagcaac ttcacggaga tttccccaac
     9661 gtttggcgtc atgaagaagt aaccgctcaa acgcaaaagt ccgcgagaac ccaggcgcga
     9721 tgtggcgggc cattaacgcg gcttccagcg gaatcgtgcc gcctccacac atggggtcaa
     9781 gcagcacgtc ggtgggagtc cagccgctca gcttgatcaa tccggccgcg agattttcac
     9841 gcagcggagc gtcgaccgtc gctattcgat gcccccgctt gaagagcggt tcgccggagg
     9901 tatcgagata gaaggtgacg gtggtggcat cgagaaacgc gtcgattttg atattgggcc
     9961 gttgggtatc caccggcgga cgtttgtggc gaacggagac gaacttgtcg cacaccgcgt
    10021 ccttaatccg gagcgtcacg aagtccaaac tcttgagcgg gcagtgacgg gcgctgacct
    10081 tgactttaat ggtctgcgac ggcagaaacc agtcaggcca ggccagcgaa tacgcagcac
    10141 gatagacatc ctcttccgag cggtaggggc tgtggccgac ctcccaaagc acgcggctgg
    10201 cgatgcgact gttgagattg acccagtaca tgctggacca cggagcatga aacgcgatgc
    10261 caccgtcggt tttggtggtg gccgggacac caagaccgtg gagttcgtct tccaggacgc
    10321 tctcgagacc tcgcgggcag ggtgcaaaga atcgatgttt tgtgctatcc atcggttgag
    10381 cctgtggtat tactggggtg cgctatgaat tattgcagcg aatgcgggaa gccggtttcg
    10441 aagaagattc ccccgggtga taatctgccc cggttcgtct gtagcacctg ccagtctatt
    10501 cattatcaca atcccaagat cgtcgcgggc tgcattcctg aatgggaaga ccagatcctg
    10561 ctctgccgac gcgcaatcga gcccaagtcc gggctctgga cgtttccggc gggcttcatg
    10621 gaaatcggcg agggaatcga acaggccgca cgacgggaaa ccatggaaga ggcccaagcg
    10681 caagtcgccg gcatccggtt acattcggtc ctgagcctgc cccacatcgg tcaggtatac
    10741 atgacctttg tgggagcctt gctcgcaaag caatacggag caggatcgga aagcctggat
    10801 gtgcaactgt tcgagcgagc caagattccg tggggcgaga tcgcctttcc tgtcgtgaaa
    10861 gaggcgttac gtcgatattt agaagatgta gcgcacggcc aatttcgcct ccacgtcgct
    10921 gacatacctg atcgttcagt ctgacgagtg tacccccgaa catagatggc cggttatgtc
    10981 cgaagctggt ttcgatgcac gatcgcgtgg atctcgagga gatgttcgac ctgatcgatc
    11041 gcatagaaga ggcgccattc gccaaccgcg tacttgtgta taccgggtaa ggctgcgtcg
    11101 acgttttcgt gtcggaggtt ctcgacgttg gacgcgatcc attttgtttt atcaaaaaga
    11161 cgttgcgcca tcggtgtgcc gatggcggcg agatcctgga cgacggcggg ctgaaatgtc
    11221 aggcgatacc aggccatcga gtgttaccag tgaaggccga gcttctcggc gatctcttcc
    11281 cccgacaatc gcgcgttgtt ggccagcgag gacttgagtc tcgttcgaag cgcttcccca
    11341 acctcaaggc cgaggtccgg gtcgccgatc aattcacgca accgatcatc aacgagaccg
    11401 cgcaccaatt ctttgagctg ttccggcgag agagaagcaa ggtccatgaa atggaaggat
    11461 acggaggcag gggatgaaat gcaatgtgct gaaccggatc agttcgaggg tgccgcggct
    11521 tcgagccgtt taataaaccg gccgagccga tcctgttccg gctgggccac gatcaggaat
    11581 tcgacgccga taccttgagc gccgcaccat cggaccgccg cattctcgat catgatggga
    11641 ttgggctcgc ccggcacgtg aaggaggagc gtgacctgca tgccggtaaa cggcgagatc
    11701 atgctctcga cgcgacaacc tctggcagaa atatcacgaa tcgatccatt gtgacggaac
    11761 tgatctcgct ggaccaaggt gctgggcatg gtaacggcaa aacgtctgtg ctttcgagta
    11821 accatatttc aatcgtttcc tctaggttaa gcggctgcag cctctaaccc ctccagaaac
    11881 tttgtgagac gatcccgttc taattcttcc attccgagaa attccgctcc gaactcgtat
    11941 tccatcgtcc aacgtaccgt cgccattcga atggtaatgg cgaaggtctg ttcaggaact
    12001 ttcagataga tcgcgaccat cgccccaact agggggggac aatccgtgac gatcttgcat
    12061 cctcccattc cgaggttgta tacgatcccc acgccggcac actcaccggc aaaggtggcc
    12121 gggaattcga ctaggtatcg gtcatgttcc cgctgatcct tgacgcccat atcactgctc
    12181 cagtgattat gcgggaaggg ccagcatgaa atgcgtaaga cgaacgactg gtacttcaga
    12241 gtgaaagcct aatggtgaag ggaacatttg tcaaagaaat cattgatttt gtaatggaaa
    12301 tacaaccatt tgtgatcggc ggccgactat gaagcgatta gaggacaagg ttgcgattgt
    12361 tacaggtagc agcagcggga ttggcaaggc tatcgcactt cgttttggcg aagagggagc
    12421 aaaggttgtg gtggcggcga ggaggaaatc gctctgtgaa gaagtggcca gaacgatatg
    12481 ccaaaagggt ggcgatgcct ttgtcattca aacagatgtc agtcatgaac agcaggtcga
    12541 tgcgctgttc gaaaagacca ttcagcagta tggtcgtgtc gatatcgtcg tcaacaacgc
    12601 ggggattttt ggagggagac ggatcgctga aacgacgacc aaggcgtttg acgatgtcat
    12661 gagtgtgaat ctccgaggaa ccttcttctg ttgtcgggcc gcgttcaaac acatgaagaa
    12721 gcagggcggg ggagtcattc tcaacatgtc cagtgttgct ggtgtgcagg cctgggcagg
    12781 aacgggcgcc tacagcgcct cgaaacacgg agtcatggcg ctcagtcgat cgttggcgga
    12841 agaaggtcgg ccgtttggca tccgggtgag cgcgatctgt cccgggggag ttgcaaacga
    12901 cttggtcgat gcgtcacaga aagagattct gaggagtgag aagatcagcc cgttcgatat
    12961 cgcagagacc gcaatctatc tcgccacttt ggggaaatat gctgtcgtcc accagatcgt
    13021 ggtggaccga tgggcggcag attggtgatg tctcccctat tcgcttcgct taccagtggc
    13081 gaccccaacg cccgtgataa cgggggcccc aatacacccg gccgtagaaa gagggtggcc
    13141 cgtagatcca ggggctgccc agatagaaac ccaatccaac tggcggatag acataggtga
    13201 aggcgtaagg ccctggcacg accaccgtcg tcgggcgttg cgacaattgc tgttgtatgc
    13261 cggccgtcgc agccgcctgt cgatcaattt gacctttgag ttgtccgacg gcttcctgtt
    13321 gcgcgcgtaa ttggccctcg agtttgccga tctgttctcg ctgttgctca atgaaggcat
    13381 taaggcagcg ggtttgggct tcatcgctgt agttggagca ttcccaggga acctgggctt
    13441 tgttggcgag agcgggagtt gccgtcaatg ctgtagcact caggcaaact gctgcgagca
    13501 accaatggtg atgagatcgc aacggtgagg cttccatccc tgtcataaga cgattcttgg
    13561 ttcccctcct tgctgaatct atacacacgt taccacgcga atcgtgctct gaacagaccc
    13621 tgcaatctca actgagatgg cgttcggaag tgcccccctg gcggtttgat tttatgacag
    13681 ttgcttcata caatgcgggt tcaagaatgc gtatagtatg actctacgac acaactctcg
    13741 taagaaggca ggccgaaaaa ggaagccatc tcaaaggccg ttcatgccgg agactggcat
    13801 gaaacgacgc ttccaacgac ggttgcttgc ctggtacgag aaaaacggcc gcgatttgcc
    13861 ctggcgaagg acggcggatc cctatcacat cctggtttcg gaagtgatgc tgcaacagac
    13921 gcaggtggat cgtgtcgtgc cgaagtatca tgaatttctc gaacgctacc ccagctttga
    13981 ggatttggcc gacgcgcccg tgactgaagt aaagcagacc tggtacccgt taggatacaa
    14041 catcaggcct gaacgacttc acggtatcgc ttgcgaaacg gttgcccggt acggaggaaa
    14101 acttcccagc gatcatgaag agctgctctc gtttaaaggg atcggacgct acacagctgg
    14161 agcgatccgt tcctttgcct tcaatgaaga cgcgccgatt ctggatacga acgtcatgcg
    14221 ggtgttgcat cgggtctttg ttgcgaaggg tgatccaaaa acccggaagg caggattgtg
    14281 ggagctttcc gaacatttga ttccacgagg caagggctac gattttaatc aggccctcat
    14341 ggatttcggg gcaacctgtt gcacggcacg gaacccggaa tgttcccgct gcccaatgaa
    14401 gtccttttgc aggtcgtatc catttaatcc caagccacgg gccagatcat gaaggttgta
    14461 gaagtcgccg caggattgat ccgccacaaa ggccgttatc tgatcgccaa acgaaaaccg
    14521 ggcgtgcatc tggccggctt ttgggagttt ccgggaggga agcgggaacc gggagagaca
    14581 ttggaagaat gtctgcaacg ggaattgttc gaagaattga acattcgcat cgataccccc
    14641 attccctttc ggatcatccg gcatgactat gccgaaaagg cggtggagtt gcatttcttc
    14701 cattgcagga tcgaaaaggg cttcgcgtct ccgctcgact gtgccgagat acggtgggtc
    14761 tatccgcatg aactcgccgg ctttcagttt cctcccgctg atcgaccgat catcgatgcc
    14821 ttgcagcgcg aaggagtgaa ttcatgaaag tcgttctcga tattgaaacg attcaggcgc
    14881 ccagggagga atgggaaagg cttgtcggca agcctcagac gaaaggtgac gccgatccaa
    14941 gcggagaagg gttcgatctc tttgctgctg gggcagccga agaagaacga cgtgccgagg
    15001 acgagcaata tgccaagtct gcattcgacg gcacgttcag ctcgatcgtc tgcatcggcc
    15061 tgctggaatt ttccgatcag atggaaccgc gaggagcggt ggcgtggtac ggtggcaacg
    15121 aacgggagct gcttcgtcaa ttttggagtc gtcttgccca ggaccgcccg agcctcttca
    15181 tcacccataa cggattaggc ttcgatctcc catttataaa gaagcgctcg atcatccagc
    15241 aggtaaagcc cagtctggag atcaatctgg ccaagttcag aaccgaaccg gtatacgata
    15301 cgatggcgat ttggagtaat tgggacacgc ggggttgggt caagctggac gtgctggctc
    15361 gcgcgttgaa cgtggagacc aagtcaggca gtggagaaca ggtcgcggcg atgtgggcaa
    15421 agggtcatgg gcttgagctg gcccggtatt gtctccaaga cacgtatgtg acgtatgcgt
    15481 gttattgccg gatgagtttt cggcagcccc tatctcgaga agtcgtgttg ctacagccgg
    15541 aacttctaga ggtcggctga ctgttttcag cgcgtcggtc gtgcctccgc ccgctttttc
    15601 ttgggggcct gcttgacgga ggctttcagg gctttgagtt cctcgtcacg ctcccgcagt
    15661 tctcgtttca tccattcaga ttccttgcgc agcacgctga tctcggtatc cttcttagca
    15721 atcgtttcct tcgccttgct gagctggtcg atctcccgtt gctggaactg gttctcgtcg
    15781 acgagtgagg cggatgccgt tggagcctgc tgagtcttag ccgaatcagc cgtcaggatc
    15841 gtcgcggtct tttgtgatgt ttttgccacc ggcatggccg ccggcatagg ggcctttgcc
    15901 aaggcttgga ggtcgatgac atgggcggtc acgcccccca tccctcctgc tgcataggaa
    15961 ggcgcgtatt ccgtgcgcgc ggctgattcc ggcgcgaacg ttgtcgagcc ggcacccttt
    16021 gagattgtga agtggatcaa cccttgccgg acatagatgc tgcccgcggt gggctcagag
    16081 cctgaccctg ccgatgagga tacgcggaac ccgacgagtt gttcgggttt cgcttttgat
    16141 aagccttgtg cgagcagggg agcaagaaac tccgcatcct catcgctgaa cactctcatc
    16201 ggcttgctgc caccggcgga cattctcgat gacccgtcgc cgtagactcc cttgacgact
    16261 ttcatgatcg tgagttgatc gatcacggct gggtgacttg cttcaaaaga ccagtccatg
    16321 acttcttcca ggtacacgct gcctttggcg ttttgagcga ttttcgatgt gctcgaacag
    16381 ccgctcatga caagcgcggc tccgaggcag acatgcatca ggagagtctt tgtcggaaac
    16441 gagcgggtcg gcataggggg ggtctccttt tccggaatga ggattggaac gataacgagc
    16501 agatgaatgt cacgtgcgat ttcgttgaag gtctaccaaa ttcccagacc catgagcgtc
    16561 atgctgagtg ccagccatcc gaagaggccc atcgcaatga tggtctcaag gctgaccgaa
    16621 gtggaagatt ccttctctgt attttgaggc tggttcgagg ctgagttcat agcgacgctc
    16681 catgatgggt agggttaagc tagtataagt ccctctaaag gcaagcaaag attgtgcccc
    16741 tagcgcttgc tcttgatggc tggtgatatc agtagggaat gggctcagaa acagaaaaca
    16801 gagttagttt gacgaaggag ctcaagcgga agaagtaact tgaaatataa ctcataactc
    16861 attgttaatt cgtggcctag cttgcgtttt ttaaaatgat gagtggagtg ccaaggtcgg
    16921 catcgagata aaacgtgcag ggctatcgac gtggaaaata ccagtagagg atagggggga
    16981 gatataaaga agactgctag ggagtgacga ttctcgtgca gctccgttcg tttttgcatg
    17041 cgggaggacg gaacgaagca cattggctag agttgctttg ccgcatcatg ccggtcttgc
    17101 ataaagcgtc gatgccgagg cgtcatagac ttccagcgag aggatctgac cgggactgca
    17161 tggcgctgca gctttttccc acacaaccgt gatgtttccg tccgttttcc ccattgcttg
    17221 caggtgagac ttcttggcgt cgccttcgac caatacgcgc aacgtagttc cgaggtattc
    17281 gcgattccgt tcagtggaga tcctgcgttg gagttccaca agtgccgtca ctcgcttgga
    17341 ctttacctgg tccgggatct catcggcgaa ttttctggcc gcgatcgtgt tcttccgttc
    17401 cgaatacaag aatacgtacg cagaatgaaa tcggacttct tcgagcagtg cataggtatc
    17461 gctaaactcg gcatcggttt ccgagcagaa gcctgcaatg acgtcggttg tcagcgcgat
    17521 atcgggattc atcgcccgaa tctgatcgac gagggccaag tattccttcc tcgtataggt
    17581 gcgattcatc atctccaaaa tacgatcgtt tcccgattgc agcggtaaat gaatgtgttt
    17641 gcaaatgtgt gggtgtgtgg ccacagcctc cagcaaggca ggagggaaat ctttggggtg
    17701 gggagaggta aagcgcacgc gttcgattcc cggcacatcg gcgaccgctg tgagcaatcg
    17761 ggcaaagtcc cattcaccga aacgata
//
LOCUS       DATIFR010000098         1610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2740267, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000098 DATIFR010000000
VERSION     DATIFR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740267"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..785)
                     /locus_tag="VLD60_05490"
     CDS             complement(297..785)
                     /locus_tag="VLD60_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44443.1"
                     /translation="MPPRAAVAAYLLLATIAACAGPLKVSSSWTSLEDVCGPVFVELI
                     PYSWVDEHYFISLDGHDPSDAYISHLKDLAHAAHRSANLKKRSQLAADQASQPLLKAV
                     NISCGNLRWEGADRVRVAAGGLIAPMPGQRFALGGSGSDFVLERRNGQWAIVAREGFW
                     VS"
     gene            complement(869..1150)
                     /locus_tag="VLD60_05495"
     CDS             complement(869..1150)
                     /locus_tag="VLD60_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HSB44444.1"
                     /translation="MKKKHLGSDFDDFLRTERLLEAADSTAVKRVIAFQIAQEMHRRR
                     LTKSEMAHRMKTSRPALDRLLDPANGSVTLSTLERAASAVGKKLKVELS"
     gene            complement(1155..1478)
                     /locus_tag="VLD60_05500"
     CDS             complement(1155..1478)
                     /locus_tag="VLD60_05500"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSB44445.1"
                     /translation="MVFYRTEAGNEPVREWLRGLPREDRRVVGQDIKTAQYGWPLGMP
                     LIRKLEPGLWEVRSRLTQGIARVLFTVDGSVMVLLHGFVKKSQKMPLSNLRTARQRLA
                     TLREE"
BASE COUNT          338 a          485 c          464 g          323 t
ORIGIN      
        1 caggagggta atgccgacaa acacgttgct gagtgtttcc gaccacacac cggccctcgc
       61 gaacaagggg gtgagcacga ggcccgcagg aagggccata aacgtgaaaa tgatacttac
      121 gagagcaggg aagactatca acacgataag gactttcatg ctgcggtgct cattcatcgc
      181 ttcctcatgc ggtggcgtcc ggtaggtccg cgattgcctc aatcgtgctt cctaacgttc
      241 ggtatcagcc gtggccgaag gccatcggtt ggatgcgatc gttagacgag ataccgtcag
      301 cttacccaga atccttcgcg cgcaactata gcccattgcc cgtttcgccg ttcaagcacg
      361 aaatcgctac cagatccccc gagcgcaaat cgttgtccgg gcattggcgc aatgagaccg
      421 cccgcggcaa cgcgcactct gtccgccccc tcccagcgca ggttgccaca agatatgttg
      481 actgccttga gcaacggttg actggcttgg tccgccgcga gttgtgaacg cttcttaagg
      541 ttcgcagagc gatgcgcagc atgagccaaa tccttgagat gggaaatgta ggcatctgag
      601 gggtcgtgcc cgtcgagact gatgaagtaa tgctcatcga cccaagaata aggaatcaat
      661 tcgacgaaga caggcccgca cacgtcttcg agtgacgtcc atgagcttga caccttaagc
      721 gggcctgcgc aggctgcaat agtggcgagg agcagatacg cggctacggc cgctcgagga
      781 ggcatttacc atcctccgtc taacgccttg cgcatgacca gcgtggcgaa gccgcgtctg
      841 tgttgatgcg cttgttgggc caaccccctt acgacagttc tactttgagc ttcttgccga
      901 cggccgaagc ggcccgctcc aaggtcgaga gcgtcacgga gccattcgca ggatccagca
      961 ggcgatcgag cgctggtcga ctggtcttca tgcggtgagc catctccgat ttggtgaggc
     1021 ggcggcggtg catttcctgg gcgatctgga aagcgatcac tcgtttgacg gccgtagagt
     1081 ccgcagcctc aaggaggcgt tcagttcgca ggaagtcgtc aaagtcactc cccagatgct
     1141 tcttcttcat cccattactc ctcgcgcagg gttgccagac gctgccgagc cgtcctgagg
     1201 tttgacagcg gcatcttctg cgatttcttg acgaagccgt gcagcaacac catcacgcta
     1261 ccgtcgactg tgaacaagac cctcgcgatg ccctgggtga gacgggaacg gacctcccac
     1321 aagccgggct ccagtttccg gataaggggc attcccagcg gccagccgta ctgcgccgtc
     1381 ttaatatcct gaccgacgac cctgcgatcc tcccgcggca ggcccctgag ccactcgcgc
     1441 accggttcat ttccggcctc ggtgcggtag aaaaccacgg acagaattgg ctctctactc
     1501 gttggaggct ccgtcatgga tggagtgtac cacaaatggt acgtacgtca atggcagcaa
     1561 gcggtccggc agaacaactc tgtgcctccg gcccgatggt cggcccaacg
//
LOCUS       DATIFR010000099         5205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2167655, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000099 DATIFR010000000
VERSION     DATIFR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167655"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="VLD60_05505"
     CDS             complement(<1..1713)
                     /locus_tag="VLD60_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HSB44446.1"
                     /translation="MALSHHLTGWLLDTYPVYVGMAVWLLADNGVRHRLIDPYTPAFY
                     LAGSHTDLTHATHLLTRRHIAHRSRMVPRRELWDAETIPVCEVAIVHPTQFHDAVMLV
                     MKSLPCLRFYQADVALPQLYFYDRRLFPFCRCEADVTGDAIIRAIQACDSPWETDYRL
                     PPLSIMELMVEGASPNPNHGGVFHLLVRVEGRERYLEGDNATEFVQTVNGLLRRYDPD
                     VLLTDWGDSYILPRLIHLAQSVGMSLELNRDTAQSVAVRSARSYFSYGRVLAQAGART
                     LYGRLHLDRHNSFALAETGLAGLFEQARVTKVPIQQMARTTTGTGITSMQLERAHQGG
                     ILIPYRKQQAEAFKTAFELLETDQGGLTYAPLSGYHEQVGELDFASMYPTIMTRFNVS
                     PETVNCVCCEDDPSARVPEIYHHTCRKRQGLVPQTLEPLLAKRARYKQQLKTITNVMV
                     RHVIDQRQTALKWLLVVSFGYLGYKNARFGKIEAHEAVTAYSREVLLRAKEIAEAQGF
                     RFLHAIVDSLWLQKQGAAREDYENLASAISAQTGLPIAVEGLYRWIGFLPSRVNPRMP
                     VHNQF"
     gene            complement(1676..1966)
                     /locus_tag="VLD60_05510"
     CDS             complement(1676..1966)
                     /locus_tag="VLD60_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44447.1"
                     /translation="MGRTLTTFTQLVQQEIASWRRYRRALRAEDQQALDDLFAAARRH
                     SAAGAYLARDMPFEVMLLSMLLEHQKHLHALSRAIADRDAHGSVPPSHGVAP"
     gene            complement(1971..2693)
                     /locus_tag="VLD60_05515"
     CDS             complement(1971..2693)
                     /locus_tag="VLD60_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44448.1"
                     /translation="MRAVAETARLPFSDRRSQEPASRVERDAVRDWFLALRHAQVPFH
                     PDDRLLLLYGHPLVFRLGLYAATERVLAGESVLYLDGANTFDPFVIGRLARAHRQQPR
                     KVLTMIHVARAFTCHQMERLVSDCLAAALDRYQARIAILSGLFDTFYDQAVPEQEALR
                     LFGRMMEGVKHLTQQGYTLLCLCPPAPILTRTSRRCLDQLRGQADRVIRVHEEQGLVK
                     LEEEGRQAGQTWEVARSVLEYR"
     gene            complement(2865..3113)
                     /locus_tag="VLD60_05520"
     CDS             complement(2865..3113)
                     /locus_tag="VLD60_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44449.1"
                     /translation="MVERNVATKEQLMDLERTVGALHGRLRDMQWEIQQAKAGSLTER
                     FWKNLMSPWQGSEFMSAVCNRRARPNTKVVDHVGTTRH"
     gene            complement(3131..3676)
                     /gene="lexA"
                     /locus_tag="VLD60_05525"
     CDS             complement(3131..3676)
                     /gene="lexA"
                     /locus_tag="VLD60_05525"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSB44450.1"
                     /translation="MKPTDLKRIREQLGLTQQQLADALRTTRVSVARYEAGMRRIPGM
                     VKVAMDQLGRSPEIPMAGMVAAGSPIEPVQQSELVDVPPSMLRGGNTFALRVKGESMK
                     DDGILPGDLVVVRKQETAQNGQTVVALVNHEATIKTYFKKPAHIELHPANAAMQPIIV
                     QPSDAFHIEGVLVGVIRHCAI"
     gene            complement(4081..>5205)
                     /locus_tag="VLD60_05530"
     CDS             complement(4081..>5205)
                     /locus_tag="VLD60_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44451.1"
                     /translation="LCDQSKGLGGMAEEVAIDIVKTFPGRARIHVELMYQLDQATSLV
                     LFGPSGSGKTTLLRCIAGLEWPEEGTIRFISRTWLDTAARIRVSPQERQIGYMPQDYA
                     LFPTYSVAGNIAYGLSELPSAARTKRVEEMIELFHLQGLERAKPGELSGGQQQRVALA
                     RAVAPRPQLLLLDEPLSALDVPTRLQLRGELRELLKQLAVPSMVVTHDWAEALTLGDA
                     IAVMQGGKILQTGSPQDVFSRPANAEVAQIVGIETVVQGQVTGNENGLATVRVNGTTL
                     KGLGQDLNGSSVYVCIRAEDVLLEQAGSGVTSARNHLAGRVTEISPQGVLVKVTVDCG
                     FPLRALITRGAKEDLGIEMGSPVVAAIKAGAVHLVGRVSP"
BASE COUNT         1155 a         1617 c         1394 g         1039 t
ORIGIN      
        1 gaattgattg tgtacgggca tgcgcgggtt gacccgggag ggcaaaaagc cgatccaacg
       61 atacagccct tccacggcaa tcggcagccc agtttgcgcg ctgatcgccg acgcgagatt
      121 ctcatagtcc tcccgggcag ccccttgctt ctgcagccac agtgaatcga caatcgcgtg
      181 cagaaaacgg aacccctggg cttccgcaat ttctttcgcg cgcaagagga cctcgcggct
      241 ataggccgtg accgcctcgt gcgcctcgat tttcccgaac cgagcattct tgtagccgag
      301 atagccgaac gacacgacca gcagccattt cagcgcagtc tgccgttgat cgatgacgtg
      361 ccgtaccatg acattcgtga tggtcttcag ttgctgcttg taccgcgcac gcttcgctag
      421 aagaggctcc agcgtttgtg gaaccaagcc ctgcctcttt cggcaggtat ggtgataaat
      481 ttccggtacg cgcgcggatg ggtcatcttc gcaacagaca cagttgaccg tctccggcga
      541 cacattgaac cgcgtcatga tggtgggata catggaggca aaatcgagtt cgccgacctg
      601 ctcgtgatag ccggagagcg gcgcataggt gaggcctccc tgatctgtct ccaacagttc
      661 aaaggcggtt ttgaaggcct ccgcctgctg cttgcgatag ggaatcagaa ttccaccctg
      721 atgagcgcgc tccaattgca tcgaggtgat accggttccg gtcgtcgtgc gcgccatctg
      781 ctgaatcggc accttcgtca ccctcgcctg ctcgaacagg ccggccaatc cggtttccgc
      841 gagtgcaaag gaattatgcc gatcgagatg gagccgtccg tacagtgttc gtgcaccggc
      901 ctgggccagc acgcgcccat aagaaaaata ggacctggct gatcggactg cgaccgattg
      961 cgcggtgtcg cgattgagct ccagtgacat ccccactgac tgcgcgagat gtatcagccg
     1021 cggcagaatg taggaatctc cccaatcggt cagcagcaca tccggatcgt accggcgcag
     1081 gagtccattc accgtctgca caaattccgt cgcgttatcc ccttccagat atcgctccct
     1141 cccttcgact cgaacaagga gatgaaagac accgccatga ttgggattgg gcgaggctcc
     1201 ttctaccatc agctccataa tggacaacgg cggcaagcga tagtccgtct cccaaggaga
     1261 atcgcacgcc tgaatagccc gaatgatggc gtcgccggtg acatccgctt cacaacggca
     1321 gaaaggaaac agacggcgat cgtagaaata caattgcggc aacgccacat cggcctgata
     1381 gaaacgaagg cagggaagcg acttcatgac gagcatgacg gcgtcatgaa actgcgtcgg
     1441 atggacgatg gcgacttcac acaccggaat cgtctcggca tcccataatt cccggcgtgg
     1501 gaccatacga gaacgatggg cgatgtgacg tcgcgtcagg agatgagtgg catgagtcaa
     1561 gtctgtatga gatccggcga gataaaaggc cggtgtatag ggatcgatca ggcgatggcg
     1621 aacgccgttg tcagcgagaa gccagacggc catgccgaca taaacagggt aggtatcaag
     1681 gagccacccc gtgagatggt gggacagagc catgagcatc gcgatcggct atcgcacgag
     1741 acagcgcgtg aaggtgtttc tgatgttcca acagcatcga gagcagcatc acctcgaaag
     1801 gcatatcgcg ggccaggtag gcgccggcgg ctgagtgtcg gcgagcggcg gcaaacaagt
     1861 cgtccaatgc ctgctggtct tcggctcgaa gcgcgcgccg ataccgccgc cagctcgcga
     1921 tctcctgctg gaccaactgc gtaaaggtgg tgagggtccg ccccatagca ctatcgatac
     1981 tccaacactg agcgtgcgac ttcccacgtt tgaccggcct gacgaccctc ttcttccagt
     2041 ttcactaatc cctgctcctc atgcacacgg atcactcgat cggcctgtcc ccgtaactga
     2101 tcaaggcaac gccggcttgt acgcgtcaga atcggagccg gcgggcacaa acagaggagc
     2161 gtataacctt gctgcgtcaa atgcttgacc ccttccatca tgcgaccgaa gagccgtagc
     2221 gcctcttgtt ccggcaccgc ctggtcatag aaagtgtcga acaatccgga caagatcgca
     2281 atgcgcgcct ggtatcgatc gagcgcggcc gccagacagt cggagaccaa tcgctccatc
     2341 tgatggcagg taaacgcgcg ggcgacatgg atcatcgtca acaccttgcg gggttgctga
     2401 cgatgggccc gagccaatcg cccgatcacg aaggggtcga aggtattggc gccgtcgagg
     2461 tagagcaccg actctcctgc caacactcgc tccgtcgccg catagaggcc caaccgaaag
     2521 accagcggat gcccgtagag gaggagcaac cggtcgtccg gatgaaaggg cacctgggca
     2581 tggcgaagcg ccagaaacca atcccgcacc gcgtcacgct caacgcgtga agccggttcc
     2641 tgactccggc gatcagaaaa tggcagcctg gctgtctcgg caactgctcg catggcacac
     2701 ctctctgtta tccgcagcac ccaccggcaa agacttgtcg ttgccgatag gcattccaac
     2761 aggtttcgca cagctctagc gtcgaatggg tcatcccgtc cacacattcg atccgctgcc
     2821 gcaccgtcat accgcaatcg gaacaggctc ccgtcattcg cttctcagtg tctggttgta
     2881 ccaacatgat ccaccacctt ggtgttcggc cgagcccgtc tattgcagac cgccgacata
     2941 aattcgctcc cctgccaggg gctcatcaga ttcttccaaa accgctccgt cagtgaaccg
     3001 gctttcgcct gctgtatctc ccactgcata tcccgcaacc gtccatgcaa cgcaccaacg
     3061 gtccgctcca ggtccatcaa ctgttctttg gtcgccacat tccgttctac catgatcgtt
     3121 ccctccttct ttagattgcg caatgtcgaa tgaccccgac caataccccc tcgatgtgaa
     3181 acgcatccga cggctgcacg atgatcggct gcatcgccgc attcgccgga tgcagttcaa
     3241 tatgtgccgg cttcttgaaa taggtcttga tcgtcgcttc gtggttaacc agcgccacga
     3301 cggtttgtcc gttctgcgcc gtctcctgct tgcgcaccac gacgagatca ccaggcagaa
     3361 tcccatcgtc cttcatcgac tcccctttca cccgcaacgc aaaggtattc ccaccccgca
     3421 acatactggg cggcacgtcg acaagttcgg actgttgcac cggttcaatc ggcgaaccgg
     3481 ccgcgaccat ccccgccatc ggaatttccg gcgaccggcc cagttgatcc atcgccacct
     3541 tcaccatgcc gggaatccgc cgcattcccg cttcgtatcg cgcaaccgac acgcgcgtcg
     3601 tgcgcaacgc atcggccaat tgctgctgcg tgagtccgag ttgctcgcga attcgtttga
     3661 gatcagtcgg cttcatgatg taaccaatgg ttacacacag gacaacacct tgtcaagccc
     3721 tgtgtttgct ctccttatat ggttgggaaa cggctgttgc ggcgcggagt ctgtgtataa
     3781 cttcaaccga aacgacaagc cggacaaggc aatgcgaatc tgacatcacg aaacatcgct
     3841 gccagcatgc gaataaccat ttgtccgctt tctccaggaa gaaccacaag atataggggg
     3901 gagatacaac acttacccaa tgcctaaaaa ataggcaaac aggtcgccgc taggctgaaa
     3961 tgcaccggta tcttcattgg cctcatcgtt acacacaggg ttatccacag aagctgggga
     4021 agagcggaac aaccaatcaa gcaggtacaa aacagagcga caaaccgcgc ttggtctctg
     4081 tcatggcgac acacgcccga ctaaatgcac cgccccggcc ttgatcgcgg cgacgaccgg
     4141 agagcccatt tcaatcccga gatcctcctt ggcccctcgc gtgatcagcg ctctcaacgg
     4201 aaatccgcaa tcgaccgtca ctttcaccaa gacgccctga ggcgatatct ccgtcacgcg
     4261 gccggccaga tgattgcgcg cgctggtgac tccgctccct gcctgctcga gcaaaacatc
     4321 ttcggcgcga atacacacgt agacggatga accgttgagg tcttggccta agcctttcaa
     4381 cgtcgtgcca ttcacacgga cggtggccaa tccgttctca ttccctgtca cctgtccctg
     4441 cacgaccgtc tcaatgccga caatctgcgc gacctctgca ttagccggtc gagaaaagac
     4501 gtcttgcggc gaaccggtct gaagaatttt cccgccctgc atcaccgcaa tggcatcgcc
     4561 taacgtcaag gcttccgccc aatcgtgcgt gacaaccatg gaaggcactg cgagttgctt
     4621 gagcagctcc cggagttctc ctcgaagttg cagccgagtg ggaacgtcca acgccgacag
     4681 cggttcatcc agtaagagca actgcggccg cggtgcgaca gcccttgcca gagccacccg
     4741 ctgttgttga ccacccgata attctcctgg tttggctctt tccaatccct gcagatgaaa
     4801 cagttcgatc atttcctcga ctcgcttcgt tcgggcggca gaaggcagct cgctcagccc
     4861 gtaggcaata ttgcctgcca ccgagtaggt tggaaatagt gcgtagtcct gaggcatgta
     4921 gccgatctgc cgttcctgcg gagacacgcg aatccgtgcg gccgtgtcga gccaagtcct
     4981 cgaaatgaaa cggatggttc cctcctccgg ccattcgagt ccggcgatgc accggagcaa
     5041 tgtcgtcttg cctgatccgg atggaccgaa aagcacgaga gacgtcgctt ggtcgagctg
     5101 atacatgagt tccacatgaa tacgcgcacg accgggaaac gttttgacga tgtcgatcgc
     5161 tacttcttcg gccataccgc ccagaccttt cgattgatcg catag
//
LOCUS       DATIFR010000100         1999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1593795, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000100 DATIFR010000000
VERSION     DATIFR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593795"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="tal"
                     /locus_tag="VLD60_05535"
     CDS             <1..1089
                     /gene="tal"
                     /locus_tag="VLD60_05535"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSB44452.1"
                     /translation="QSIWMDFIRRGMISSGELKQLIEEDGLRGVTSNPSIFEKAITGS
                     NDYDDAIRALALEGKNVDQIYSALTVEDIQGVADLFRPVYDRLDGTDGFVSLEVSPHL
                     AYDTVGTIADARRLWVAVARPNVMIKVPGTIEGLPAIRQLIGEGINVNITLLFGLPRY
                     RQVAEAYIAGLETLAVQNKPMKSVASVASFFLSRIDTLLDPMLEKTMAVDKSKADFVE
                     NIHGQVAICSAKAAYRIYKEIFNSERFRKLKEQGARIQRLLWASTSTKNPAYSDLKYV
                     EPLIGPETINTVTLETLAAYRDHGKPASRLSKGGVKAAGTLRRLSKAGIDLDRATQQL
                     EDEGVEKFGKAFDVLLNSLEEKRVAAIR"
     gene            1302..>1999
                     /locus_tag="VLD60_05540"
     CDS             1302..>1999
                     /locus_tag="VLD60_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HSB44453.1"
                     /translation="MDKTRFNYLYVFLAMLGVLLLHDAIVRFQQVAPIPYSDFQRLLR
                     EGKVKEVVVLADRIQGELKEPEGGKKRFVTNRVEQDLVAELSRHDVKFTGHVESTWLP
                     TLLSWIVPVALFAGIWIYMSRRMAGRIGADGLMSIGKSKAKVFVETDTKVTFADVAGV
                     DEAKQELQEIVTFLKDPGQYGRLGARMPKGVLLVGPPGTGKTLLARAVAGEAGVPFFS
                     ISGSEFVEMFVGVGA"
BASE COUNT          461 a          549 c          604 g          385 t
ORIGIN      
        1 caaagcatct ggatggattt catccggcgc gggatgattt catcgggtga actgaaacaa
       61 ctcattgaag aggacggtct gcgcggcgtg acctccaatc cgtccatctt tgaaaaggcc
      121 attacaggca gcaacgatta cgacgacgcc atccgcgcac tggcgctgga aggcaagaat
      181 gttgaccaga tatactcagc cctcacggtg gaggatattc aaggtgtggc cgatcttttt
      241 cgtcccgtct atgatcggct ggatggtaca gatgggtttg tgagcctgga ggtctctccc
      301 cacctcgcgt atgacaccgt ggggaccatc gccgatgcac ggcggctatg ggttgccgtg
      361 gcccgcccga atgtaatgat taaagttcct ggaaccattg aaggattgcc ggcgattcga
      421 caactcatcg gcgagggaat caacgtcaac atcactttgc tcttcgggct tccccggtat
      481 cggcaggtgg ccgaagcata catcgccggc ctcgaaactc tagccgtaca aaacaaaccg
      541 atgaaatccg tggcctccgt cgccagcttc ttcctcagtc gaatcgacac gttgctcgat
      601 ccgatgctag agaaaacgat ggcagtggac aaatcgaagg ctgatttcgt cgaaaacatc
      661 cacggacaag tggcgatctg cagtgccaaa gcagcttatc ggatctacaa agaaatattt
      721 aacagtgaac gcttccgcaa gctcaaggaa caaggcgcga gaattcaacg cctcctgtgg
      781 gccagcacaa gcaccaaaaa tcccgcttac agtgatctga aatacgtgga acctctcatc
      841 gggcctgaaa ccatcaacac ggtcacgctt gagacactcg ccgcctatcg agaccacggt
      901 aaaccggcgt ctcgtttgag caaaggcgga gtgaaggccg ccgggacact ccgacgatta
      961 tctaaagcag ggattgatct ggacagggca acccagcaac tcgaagacga aggcgtcgaa
     1021 aagttcggca aggcattcga tgttttgttg aactcgctgg aagagaagcg agtcgcagcg
     1081 atacgatagt atgaaaagga atacttccaa gcggtcggtt ccagccaaaa ccgtttttct
     1141 gttcgacgtg gacaatacgc tgccatcatt caggggggca ggtcagttgg tataaaaagg
     1201 ggggcaggtc agaggttgaa cggttgaaac ctccctcggc ggggcgtgca tctaaggcgt
     1261 tatttcaggc gcaatacccg ataccgggga ggaaacgggc cgtggacaaa acccgcttca
     1321 attacctgta cgtattcctt gcgatgctgg gagtcctcct cctccacgac gctatcgtgc
     1381 ggttccagca ggtggcgccc ataccataca gtgactttca gcggctgctc cgggagggaa
     1441 aggtcaagga agtcgtcgtt ctggccgacc ggatccaggg cgaactcaag gaacccgaag
     1501 gggggaagaa acggttcgtc acgaaccggg tggagcagga cctcgtggcc gaactctccc
     1561 gccacgacgt caagttcacc gggcacgtcg agagcacctg gctccccacg ctcctttcct
     1621 ggatcgtccc cgtcgcgctc ttcgccggga tctggatcta tatgtcgcgc cgcatggcgg
     1681 gccggatcgg ggcggacggg ctcatgtcca tcgggaagag caaggcgaag gtattcgtcg
     1741 agacggacac gaaggtgacc ttcgccgacg tcgccggggt ggacgaggcg aagcaggagc
     1801 ttcaggagat cgtaaccttc ctcaaggacc ccggccagta tggccgcctg ggcgcgagga
     1861 tgccgaaggg ggtcctcctc gtggggccac cggggacggg gaagacgctg ctggcgcgcg
     1921 ccgttgcggg agaggcgggg gtcccgttct tctcgatcag cggctcggag ttcgtcgaga
     1981 tgttcgtcgg cgtcggcgc
//
LOCUS       DATIFR010000101         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2169010, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000101 DATIFR010000000
VERSION     DATIFR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169010"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VLD60_05545"
     CDS             <1..265
                     /locus_tag="VLD60_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSB44454.1"
                     /translation="TAYADDTQFDKRSHHYDAASTKAAPRWDMVDIKYRERFKRSLPL
                     DQLRREPKLKGMVLLRKGSRLSVQPVTESEWAVIHKLAQSARE"
     gene            complement(310..561)
                     /locus_tag="VLD60_05550"
     CDS             complement(310..561)
                     /locus_tag="VLD60_05550"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HSB44455.1"
                     /translation="MVLRIPSRVALPFGYRISVRQLSDSEMDRRDPNADGIWDDETKT
                     IYLRKRLPVTRRRYILAHELGHAWLDWQHRHLDNGKAKT"
     gene            825..1904
                     /locus_tag="VLD60_05555"
     CDS             825..1904
                     /locus_tag="VLD60_05555"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSB44456.1"
                     /translation="MMATRITLPVGVAGVSLAVGLWLGASILPVDASPSGYAPHGTVT
                     IDGITVADIGPLPTSVPIPSTNLNYAAKVELGKQLYFDGRLSKNGAIACAFCHNPGTG
                     WADPRQTSIGIGGGLGGRQAPTVLNTAFNHLQFWDGRARSLEEQAIGPIHNPIEMGET
                     HEHVVAKLNKIKGYREQFRAVFGTDVNLQGIAEAIAAYERTVISTNSPFDKYVGGDRT
                     AMNEAAVRGMALFKGKARCILCHSGSNFTDNQFHNLGVPQVGPMKEDLGRFYVTKAEK
                     DKGAFKTPTLRSITETAPYMHDGAFKTLEEVVDFLNNGGGAHPNLSAMVKPLNLTAEE
                     KTDLIAFLKALTGEAIKVSLPRLPK"
BASE COUNT          498 a          544 c          605 g          451 t
ORIGIN      
        1 gacggcctat gccgacgaca cgcaatttga caagcgaagt catcactatg atgccgccag
       61 taccaaagcg gctccgcgat gggatatggt cgatatcaag tatcgggaga gattcaaacg
      121 tagcctgcct ctggatcaac ttcgacggga acccaagctg aaaggtatgg tgttattacg
      181 aaagggctcc cggctgtccg tgcaaccggt gacggaatcc gaatgggcag tcatccacaa
      241 attagcccaa agcgccaggg aataagcaat cagacgagag agttgaaagg ggccgtagct
      301 gtcgagaggc taggtcttgg cttttccgtt gtccaaatgg cgatgttgcc aatcgagcca
      361 cgcatggcca agctcatggg cgagaatata gcgccgcctg gtgaccggga ggcgttttct
      421 gagataaatc gtctttgtct cgtcgtccca gatcccgtcg gcattcggat cacggcgatc
      481 catttcagag tcggagagtt ggcgaacgga gatccgatat ccaaagggca gcgcgactcg
      541 tgagggaatt ctcaacacca tttgaaaagg ctcctctttc ccgattagcc taccacaaac
      601 atttcaagcg attcatggca aaacaatcag aaatatcaag tgcttatgcg atcatcgcct
      661 ctgaggtcgc ctagatcgat ctcactctgt gacctgcctg tctccctggg gaaggaggcc
      721 aagattcgat gtttctcaaa ggccgcctga cctcgcctcg tgatagtacg tatgctattc
      781 tgccggttcg aatgaggcct tttctgatgc acaaccagga ggggatgatg gcgacacgaa
      841 ttactctgcc agtcggtgtt gcaggcgtga gcctggcggt cgggctctgg ctcggcgcga
      901 gtatcttacc ggtagatgcg tctccgtctg gctatgcgcc tcatggtacg gtcacgatcg
      961 acgggattac ggtggcggac atcgggccgt tgcccacatc ggttccgatc ccctcgacga
     1021 acctgaacta tgcggcgaag gtcgagttgg gtaaacaact gtacttcgat ggtcgattgt
     1081 cgaagaacgg ggcgattgcc tgcgcctttt gccataaccc cgggaccggg tgggccgatc
     1141 ctcgtcagac ctcgatcgga ataggcggcg ggctcggcgg ccgtcaggcg ccgacggtgc
     1201 tgaataccgc cttcaatcat ttgcaattct gggacggccg ggctcgctcg cttgaagaac
     1261 aggccatcgg ccccattcat aatccgatcg agatgggcga gacccatgaa catgtcgtgg
     1321 ctaaattgaa caagatcaaa gggtatcgag agcaatttcg tgcggtgttt gggacggacg
     1381 tcaacctgca gggcatcgcc gaagccatcg cggcctatga gcgaacagtg atttccacca
     1441 attcaccctt cgacaagtac gtagggggag accggacggc gatgaacgaa gcggcggttc
     1501 gaggcatggc tctgttcaaa ggcaaggcac gctgcattct ctgtcacagc gggtccaatt
     1561 ttaccgacaa tcagttccat aacctcggag ttccgcaagt gggtcccatg aaagaagatc
     1621 tcggccggtt ctacgtgacg aaggcggaga aggacaaagg cgcgttcaag actccaacgt
     1681 tgcgaagcat caccgagacg gccccctaca tgcacgacgg ggcgttcaag acgctcgaag
     1741 aagttgtcga tttcttgaac aacggcggtg gcgctcatcc caatctgagc gcgatggtca
     1801 aaccgctgaa tctcacggcg gaagagaaga cggacctcat cgcgtttcta aaggctctca
     1861 ccggagaggc gatcaaggtt tctttgccga ggttaccgaa gtgataagga aatctgacgt
     1921 tggctgtccc taacgcatca agattgaccc ggcgagaaaa aatgtgaatc ttttcccttc
     1981 ggcatggctc tgagagaggt gagtgccatt tctagagcaa gaggtcaggt ctgtttaatt
     2041 tcatccttca acccagagga ggtttattgt gatgagaaaa agactgctag ccgttgtc
//
LOCUS       DATIFR010000102        20632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1213261, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000102 DATIFR010000000
VERSION     DATIFR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213261"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="VLD60_05560"
     CDS             complement(<1..1595)
                     /locus_tag="VLD60_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSB44457.1"
                     /translation="MSSPDLEEALRRSEEFAARLIESSRDCIKVLDLKGHLLSMNAHG
                     MTALEICDFGPLVGSPWVEFWEGEDKKNARAAIDAAREGKVGRFTGFFPTTQTRTPKW
                     WDVSVTAILAKDGKPDKLLAISRDVTELKEAEQLLLRSQEELEEQIHVRTRELAQTGA
                     VLQDIVEGVEAKVGEQFFPSLVQQLATSLGVDYAYISELSEDGATFRSRAGWGKGQAL
                     PPFDVPARGPCETVLTRKCVHHPDQLRELYPHVQLIQQINVNSYCGVPVVDSNDRVLG
                     HLAIMDSKPMPDHVRATSILGIFAMRAAAEFERLRFETTIRKSDLTLRKIDEGTAGAT
                     GAAFFPSLVRNLAEALQTKYAFVSKFVEGNRARVRTLAFWHGDGFLENFEYDLPHTPC
                     ERVLAGEVCLFPEKVQDLFPDHRKELAELGVESYLAIPVSDRSGTVMGHLAVMDTKPM
                     HDDPRVLSVFKIFGVRAGAELERERMDFQLKDNEERLRDLFDEAPIAYVNEGLDSKFI
                     RANRTAMKSLGITPDQVEGTYGAS"
     gene            complement(1907..3094)
                     /locus_tag="VLD60_05565"
     CDS             complement(1907..3094)
                     /locus_tag="VLD60_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSB44458.1"
                     /translation="MVAQTLDTALEAVDAECGSILIADPASQQLVNYRCVGAKPVPPG
                     SANPWHQGIAGAVFKSAQHAIIVDAETDPLLPSRVDKATGSVIKNMIILPLKRWQGEP
                     IGVIEILNKRSGPINDDDVAVLTVISSLATPVIEEARLFEAKKLAEVAQLAGDMSHDI
                     KNLLMPVLCGAGVLKDELDELFGKLSDRDSPQSAKSRALCNEVLDMVQEDVRRISDRV
                     RELADCVKGMSAPPKFAPCCVANVVLSVFKTLELMAKKKGVTLSSEGLDRLPAIEADE
                     QRLFNAFYNLVNNAIPEVPSGGSVTILGKAPLTAEVVDISVTDTGRGMPPEIRDSLFT
                     AHARSQKAGGTGLGTKIVKDVVDAHKGHINVESTMGKGTSFRLILPLRQTQASQYNDS
                     AAA"
     gene            complement(3297..3704)
                     /locus_tag="VLD60_05570"
     CDS             complement(3297..3704)
                     /locus_tag="VLD60_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44459.1"
                     /translation="MGLGRILVVDDEAHIRTTVKMVLTKAGYDVVEAEDGEKGIHAIK
                     SGDNPLMVDMILCDMQMPKIDGIGAIAHFLSQFPSVPIVVMTGHPDVQAATKLMKQGI
                     MDYLVKPIEKDKLLAVTEEAVKKHILFKDHFGA"
     gene            complement(3857..4612)
                     /locus_tag="VLD60_05575"
     CDS             complement(3857..4612)
                     /locus_tag="VLD60_05575"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HSB44460.1"
                     /translation="MTTWIKVIVGILAVAAGWWGLLSIDYERYLTPTALAQWLNNAGP
                     WAPFLLIGSMVCAVIIPPIPSLPLDLAAGAAFGPFYGALYAVIGAEIGAIACFLVARA
                     LGRDALSRFMKTETTFCQMCTDHQLMGAMFLARFIPIFSFDVVSYGAGLTNISLKSFS
                     LATLFGMAPPTFAFVYLGSSVVTAQWPLITAGVAMVLFFLAMPKLLTTSRTSAFARLL
                     LGPPPAPVMIASDGNEILSHTRCTGCGATVEKS"
     gene            complement(4651..5685)
                     /locus_tag="VLD60_05580"
     CDS             complement(4651..5685)
                     /locus_tag="VLD60_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB44461.1"
                     /translation="MGLKLLRILTLVSLSLLTPYRSPFTAAAAPPVVVQSVASVGFTV
                     SDMDRSIAFYRDVLTFKPVSDVEVDGPEYDRLWGVFGVRARVVRMQLGGQQLELIEFL
                     SPPDVRPIPVPSYSNDLWFQHFAIVVRDMDAAWARLRQHHVRQISPRPQTIPISNQAA
                     AGIKAIKFRDPDGHNLELLWFPDGKGNPVWKEPGSNLFLGIDHTAMTVRNTESSIKFY
                     RDLLGLTMAGGTLNMGTEQEHLDSLPGARARVTGLATKMGPPGVEFLEYELPTAGKPY
                     PTDSHPTDLWHWQTTMMVSDIEAAAATLRGKAQFVSSGVASIADGNLGFKKGFLVRDP
                     DGHVLQVISP"
     gene            complement(5690..7246)
                     /locus_tag="VLD60_05585"
     CDS             complement(5690..7246)
                     /locus_tag="VLD60_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSB44462.1"
                     /translation="MASHYDIIIIGTGPGGGTLAYKLAPSGKKILLLERGGYLPREKD
                     NWNTKTVFIENRYKAKETWKDKDGGTFHPGIHYNVGGNSKVYGAALLRMRAQDFGEVK
                     HHGGISPEWPIRYDDLEPYYTQAEHLYHVHGNHGEDPTEPKASAPYRYPALTHEPRIQ
                     ELHEDWQKCGYKPFHLPVGIMLNEQRPEQSACIRCNTCDGYPCLVNAKADSQVVCVDP
                     ALQYPNVTMITHALVTRLETSGTGREVTGVIVERNGQKETYKSSIVVVSAGAINSAAL
                     LLRSANDKHPNGLANSSDMVGRHYMCHNNSAMLAISKTPNPTVFQKTIGLNDFYHASA
                     DWDFPMGHISMIGKSDMDTLRAGAPAFAPGFALDQMAKHSLDFWMTSEDLPDPNNRVL
                     VGKDGGITLAYTENNLEAHQRLAAKLKSMLNHIGCEDHLLPTHLYLGKKIPIAGTAHQ
                     CGTVRFGRDPKTSVLDIHCKAHDLDNLYVVDASFFVSSSAVNPSLTIIANALRVGDHV
                     LQRLGVRSEG"
     gene            7745..8305
                     /locus_tag="VLD60_05590"
     CDS             7745..8305
                     /locus_tag="VLD60_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44463.1"
                     /translation="MEDEHDQESVDTRTAAAAVVPSACDAKPIYFPVSLTKFVVMNFC
                     TFGMYQFYWFYENWKFIREREQSDVSPFWRTAFAFIYCYPLFEKIWSSAASLEIGQPF
                     SVPFLATGWILSSLLGVLPDPYAMAAFLSFLFLIPVQQTANRINESLAPGHDRNDRLT
                     AWNTVAVVIAALLFILVVIAIIISRE"
     gene            8456..9004
                     /locus_tag="VLD60_05595"
     CDS             8456..9004
                     /locus_tag="VLD60_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44464.1"
                     /translation="MAMDWSVLIGLLALVIVGATASFYVHAFVVRTRNAFRNRQPNLR
                     ITNLSALNSGDVLTLCPELENVGGGVAYDCVMHLGGWEGSFAVKSLHPRGPRYQKHLI
                     SIVLGPDAPIRVKPMTNDFLRLRYRDRWGFTYECWYHVAQIRSGSMRFHNVHIDLEHP
                     DVTEPHPSFWEMWKLLRTSSPT"
     gene            9158..9667
                     /locus_tag="VLD60_05600"
     CDS             9158..9667
                     /locus_tag="VLD60_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44465.1"
                     /translation="MNRYYVVIVLVGLLTLVGCISHPQTAEEFRKAVPGAFSAKVETF
                     EVDRPFSQVASTFQKMGPDCLSKTIKTTSQTNRSYQVIVTTYKPTVLVTGKKAELHVQ
                     QHHEQGVLNVSKEPDGGYYLLVADAQPVTQTKTRIDLYRQSIGHGVLIQAVKNWATGE
                     NVGCPDLTK"
     gene            9646..10200
                     /locus_tag="VLD60_05605"
     CDS             9646..10200
                     /locus_tag="VLD60_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44466.1"
                     /translation="MSRSDEVTPLARNRAAGNPLGTVRIAFVAFSICTASLLAGCFGS
                     KTYPNTLDRNLHVHTATDSGSWFSHLRAAVDIHRVGEDCALGYEGTVQLTDPKIDIGI
                     PPHRWSYLVFVFDSSSFLGNRSGTITYETLLKPRSNYHYDVAVRYRDEMYHVEIRETP
                     PDQSAGRELDRLAFSACRSSSGRK"
     gene            complement(10345..10890)
                     /locus_tag="VLD60_05610"
     CDS             complement(10345..10890)
                     /locus_tag="VLD60_05610"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44467.1"
                     /translation="MRQRVSVTTSVLVSVLAIGVAIPMVFAEEFSRDSAARYILATAK
                     AFRTVYAKQVVAQAAKSNVKPDENWTKDDHSIMLPAQFLKAAGTELKDFELSLIGLTP
                     IYKENLPKSKAEEEALKKLAANPATGVLTFEDGKQFKGIAADLAIADSCVSCHNAHPM
                     SPKKDFKKGDLMGAIVVRFNK"
     gene            complement(11023..11478)
                     /locus_tag="VLD60_05615"
     CDS             complement(11023..11478)
                     /locus_tag="VLD60_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HSB44468.1"
                     /translation="MQGSRFQILEEYKGEAVLDTETHLIWERTPSSGETAWANASLRC
                     ALTSTGGRLGWRLPSFLELMTLVEPAPLATATKPSLPASHPFRGVRADPYWTTSSQDA
                     DPTNAYAIDFLRGDVTSQRKNQAHAWWCVRGGASGPPAMSARVRPQESI"
     gene            11956..13239
                     /locus_tag="VLD60_05620"
     CDS             11956..13239
                     /locus_tag="VLD60_05620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB44469.1"
                     /translation="MARKRIVIIGGGFAGLAAARSLRHADVVLIDRTNHHLFQPLLYQ
                     VATAALSPSDIAWPLRTVFRSQSNIRVVMDEVLSIDRPARLVRLRDSAPMAFDVLIVA
                     PGSRHAYFGHDEWEPFAPGLKTMTDAVRLREKMLLAFEDAERRRAATGMQDRLTFVIV
                     GGGPTGVELAGALAEIGRKAMGPDFPTLRLDDLSIVLVEGGPRILPGFSEDLSVKASA
                     ALTHMGVTIKLNSPVSAVRADGVMIGNELVASTNIIWAAGNRASPLLNTLSVPQDASG
                     RVKVRSDLTIPGDPWIFVIGDAAHCVGVDGTPLPGLAPVAMQQGRYVAKLIAHDTAPE
                     QRRPFVYAGRGMLATIGRAQAVAQLGPVHVSGLFAWLLWCVVHIFFLIGFRNRVRVMS
                     EWMWYYITFKPGARLLFERPREPRNGPMPRDHSHE"
     gene            complement(13432..13893)
                     /locus_tag="VLD60_05625"
     CDS             complement(13432..13893)
                     /locus_tag="VLD60_05625"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HSB44470.1"
                     /translation="MTIASKFLVSIMVLVASMASASAFAQDFSHSTPGISGYDPVAYF
                     TDGKPIRGSGFHVTVVDGVTYAFANAEHQKMFEANPQKYLPAYGGYCAYGVGVGKKFV
                     ADPEVWRIVEGKLYLNLDKSIQQKWEKDIPGYIKKGDVNWATIKDKKPSDL"
     gene            14490..14690
                     /locus_tag="VLD60_05630"
     CDS             14490..14690
                     /locus_tag="VLD60_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44471.1"
                     /translation="MGQGMVTDQILEFVKAHPNCTLDEVTLRLQEWNWSEVFIEVDRL
                     NRLGRLRLIRSGTGFVTRLSAL"
     gene            15104..16300
                     /locus_tag="VLD60_05635"
     CDS             15104..16300
                     /locus_tag="VLD60_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HSB44472.1"
                     /translation="MIDQTPILIVDDSPDDLVLLDQMLRAHGYSTIPASSGQEALKKI
                     ESHSPQLALLDIMLPDMNGYEVCKLIRQDPRTALLPVIMVTALREDRITGIEAGADDF
                     LVKPINRDELIARVRSLLRIKSLHDAVKGHVGELAEWNKKLEIRLAQEAKLAEVGRVL
                     GDISHEIKNLLMPIVTGTGLLHEELKDIFALMPQKGADKLRASEKQCAEIEEMLLRAA
                     NRLHEHVQEIADCVRNISSPPAFAPCSIASVADGVFKTLRVVAEGKDVSLCLDHLTDL
                     PPIQADERRMFNVFYNLINNALSEVPPGGSITIRAAHEPDSGTVCISVKDTGPGMPPE
                     IRDRLFREGVISRKAGGTGLGTKIVKDVIDAHGGRIRVESELGHGTTFFITLPIEQVA
                     RTASFD"
     gene            16492..17175
                     /locus_tag="VLD60_05640"
     CDS             16492..17175
                     /locus_tag="VLD60_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44473.1"
                     /translation="MGFSLCVLVLLMLSGCGGFRGGIESVPYVGDDEPQQTSTNRSWP
                     HEINLPGLTLYLSLNNTVQSYQYEVMLYIIPTYLNFWDEFQHGDAENVELTLQMTPHE
                     ASISIDPRKLVLTVDDREFRPIAVWVNNLERERQVIDAYVKARRQAPPDQPPSIPSSS
                     EWRDSVMAPVIIRPREKSPRFIVTFPAQLPSPERTLSLNLGTAIIEPLSFDIPLIRFK
                     PMHWSEGYS"
     gene            17359..18405
                     /gene="ada"
                     /locus_tag="VLD60_05645"
     CDS             17359..18405
                     /gene="ada"
                     /locus_tag="VLD60_05645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HSB44474.1"
                     /translation="MPSVDTGSAWDAVLKHDCRFDGSFVYAVFSTRIYCRPSCPSRRP
                     TRRQVTFFKSPQEAEAAGFRACLRCRPQSTAGSEIETRVVGLRRYLDEHADEPVTLQQ
                     LAKEAGVSPFHLQRTFTRIVGLSPKAYQDARRMERFTLSLKQGATVTHATYEAGFGSS
                     SRLYERVNATLGMTPSALRSGGTGVTLRYATVATPVGRLLVAITERGVAAVSLGDTES
                     WLVASLRQEFPKARLRKDPDGLRPYLQAMLQCMTGYGDLAQLSLDVNATAFQRKVWQA
                     LQRIPRGETRSYQEIARTIGQPTASRAVARACAKNRIAVAIPCHRAIRGTGQLAGYRW
                     GLERKKQLLDLERA"
     gene            18402..19106
                     /locus_tag="VLD60_05650"
     CDS             18402..19106
                     /locus_tag="VLD60_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44475.1"
                     /translation="MSERTFRSASMFPFVAGPLEARTAGAYSEGILGALRMRAIFVII
                     VACLAAGCLSTTREREVHCLSAMMMDTWQAKNDVESAERAWRTAQQARFERSRAHRDS
                     SVLSVLIDNRYPSSGTVASDSLQGDGAGNRPPEIDEERTLYHRVAAAQARHRETVVWY
                     GRVAHRVQTRIEEDDMLYPVLGMLATSTGIVLYPLIRWNVRSVLWEGVDPDADDDPVQ
                     VYCAARLGHENPGPPP"
     gene            19275..19484
                     /locus_tag="VLD60_05655"
     CDS             19275..19484
                     /locus_tag="VLD60_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44476.1"
                     /translation="MKTLGLAVLAAIGGYLIGLFGGMLLVETFSSNQHDKSVEAAMTG
                     AFVVGPLMAVVAVIVLLIFRSRRAQ"
     gene            19518..20183
                     /locus_tag="VLD60_05660"
     CDS             19518..20183
                     /locus_tag="VLD60_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44477.1"
                     /translation="MIHMLQRIVGSVAVRDLLGNAVLGLILLGLLPSPLDAARGGGGK
                     TEVLPVEKAAPAVVVVEASGSEQELRAQLRKKSGVVELNVPQSKFSEAPTGSFGFIAP
                     QFLGMALVTQSPDLLINKVAPTANAYEIHKLADGSGLLIGFIAKDLAPQVTPASRPHH
                     VRIALYSTPSDKAPLIAALPMIKLMVDRLPVKLDPKKADGPVMLDMDLQGTANRKSVP
                     GTQ"
     gene            20417..>20632
                     /locus_tag="VLD60_05665"
     CDS             20417..>20632
                     /locus_tag="VLD60_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44478.1"
                     /translation="MKTSLMLFIALLWMSAPAFANPDMLPKHPGYPMGKAVDPVKGQP
                     LANDPGQGNATGEGSLSKAAAFDDAHVK"
BASE COUNT         4092 a         5868 c         6018 g         4654 t
ORIGIN      
        1 gaggcaccat aggtgccttc cacctggtcc ggtgtgatcc ccagcgattt cattgccgtt
       61 ctgttggccc ggatgaactt ggaatcgagg ccttcattga cataggcgat gggggcttcg
      121 tcaaacaggt cgcgcaagcg ctcctcattg tctttgagct ggaagtccat gcgttcacgt
      181 tccaactcag cgccggcccg gacgccgaaa atcttgaaga cggagagcac gcgcggatcg
      241 tcgtgcatcg gctttgtgtc catcacggcg aggtgtccca tgaccgtgcc ggaccggtcc
      301 gacaccggaa ttgccaggta gctttccact cccagttcgg cgagttcttt ccggtggtcg
      361 gggaacaggt cctgaacctt ttcaggaaag agacacactt cgccggccag cacgcgttcg
      421 cagggggtat gaggcaggtc gtattcgaaa ttctcgagga agccgtcgcc gtgccagaag
      481 gccaatgtcc gcacgcgagc ccgattacct tcaacgaatt tggagacgaa cgcgtatttg
      541 gtctggaggg cttcggccag gttcctgacc agcgacggaa agaatgcggc cccggtggct
      601 cccgccgttc cttcgtcgat cttgcgcagg gtcagatcgc tcttgcgtat ggtggtctcg
      661 aaccgtaacc gctcaaattc cgctgcggcg cgcatggcga agatcccgag gatggaggtg
      721 gcgcgcacat ggtccggcat cggcttggaa tccatgatgg ccagatgtcc gaggactcga
      781 tcgttggaat ccacgaccgg cacaccgcag tagctgttga cgttgatctg ctggatgagc
      841 tgcacatgcg gataaagctc gcgcaactga tcagggtgat gcacgcactt tcgcgtcaac
      901 accgtttcgc agggaccgcg cgccggaacg tcgaatggag gcagggcctg gcccttgccc
      961 catcccgctc tggagcggaa cgtagcaccg tcttcactga gttccgagat ataggcgtag
     1021 tcgacaccca gcgacgtggc cagctgctgc acgagcgagg ggaaaaactg ctcgccgact
     1081 ttcgcttcca cgccttcgac gatgtcctgc aggaccgcgc cggtctgagc cagctcgcgg
     1141 gtgcgtacgt ggatctgctc ttccaactcc tcctgcgagc ggagcagcag ctgctcggcc
     1201 tccttgagtt cggttacgtc gcgggaaatc gccaggagtt tgtccggctt tccgtcttta
     1261 gcgaggatgg cggtaacgga gacgtcccac catttcggcg tcctggtctg agtcgtcggg
     1321 aaaaatccgg tgaagcggcc aaccttgccc tcgcgggcgg cgtcgatcgc tgcgcgggcg
     1381 ttcttcttat cctcgccttc ccagaattca acccaggggg agccgaccag cgggccgaaa
     1441 tcgcagatct ccaacgcggt catgccgtgg gcgttcatgg agaggagatg acctttgaga
     1501 tcgaggactt tgatgcagtc gcggctgctt tcgatcaagc gagcggcaaa ttcttcgctg
     1561 cgcctgaggg cttcttcaag gtcgggagag gacatcggca tcaccttgcg atgggcctga
     1621 tggggaaggc gaaacagagg cgaagtctag catcggaggg gaaatagctg caactaaggg
     1681 caaattgtta ggaaaggatt gcggtcgagt gtccaaatca tgcctccggt accgtcatcc
     1741 cgcagccggt gcagcgaacc cgcaacggac tcttgtttct gtccaaggcg atcaccaccg
     1801 gtgccggggt atggaccgag aagtagccgc gccgacgggc ttgtccggta tgtcgtcagc
     1861 attgtggcat ggccaaagaa tgcgaccctg acaacgcccg ctgcagtcac gctgccgctg
     1921 agtcgttgta ttgactggct tgtgtctgtc ggagagggag gatcagacgg aagctcgtgc
     1981 ctttacccat tgtactctcg acgttgatat ggcctttatg ggcatcgacc acatccttga
     2041 cgatcttggt tccgagtcct gttccaccgg ctttttggct gcgggcgtga gcagtgaaga
     2101 ggctgtcacg gatttccgga ggcattccac gccctgtatc ggtcacggaa atgtccacga
     2161 cttcggcagt cagaggtgcc tttcccaaaa tggtgaccga gccgccggag ggaacttccg
     2221 ggatcgcatt attcaccagg ttgtagaagg cgttgaagag tcgctgttcg tctgcctcga
     2281 tggcaggcag cctgtcgagg ccctcgctgc tgagcgtgac tccctttttt tttgccatca
     2341 actccagggt tttgaacaca ctcaacacca cgttcgcgac gcaacatggc gcaaatttgg
     2401 gcggcgcgct catgcccttc acgcagtccg caagctctcg aacccgatcc gagatccttc
     2461 gaacatcctc ctggaccatg tccagcacct cattgcacag cgcacgactt ttcgccgact
     2521 gaggcgaatc tcgatcggac aattttccga ataactcatc gagctcgtct ttgagtactc
     2581 ctgccccaca gaggaccggc atgagcaaat tcttgatatc gtggctcatg tcaccggcca
     2641 gctgcgccac ctccgccagt tttttggctt caaataacct cgcctcctcg atcaccggtg
     2701 tggcaaggga cgaaatgact gtcaggactg cgacgtcatc gtcattgatc ggaccgctgc
     2761 gtttattgag aatctcaatc acgccgatcg gttcgccttg ccatcgcttg agcggcaaga
     2821 tgatcatatt cttgatcacg gatcctgtgg ctttatcgac tctgctaggc aacaggggat
     2881 cagtctcggc atccacgatg atggcatgct gggccgattt gaacaccgca cctgcaatac
     2941 cctggtgcca cggattggct gatccaggcg gaaccggctt tgccccgacg caacgataat
     3001 tgaccagttg ttgagatgcc ggatcggcaa tcagaatgga cccgcattcc gcatcaaccg
     3061 cttccagagc ggtgtccagg gtctgcgcga ccaaatcttc taatcgggtg tattgtgaga
     3121 gtgctcgaga gataaggagg gttgtctgta attctctatt tccctgggca aaatgaaggt
     3181 ccgccattcg ccggggagcc cggcggcggt cttgatgtgc gttaggctct gggggcagtt
     3241 tctcctcagg aagcacccag tcatcctttc aacggcctca gtgaactgcg cggcgattac
     3301 gcgccgaaat ggtccttgaa gaggatgtgt ttcttgaccg cttcctcggt gacagccaga
     3361 agcttgtcct tctcaatcgg cttgaccagg taatccatga tcccctgctt cataagcttc
     3421 gtggcggcct ggacatccgg atggcctgtc atgaccacga tcggaacaga aggaaattga
     3481 gagagaaaat gggcgatcgc tccgataccg tcgatcttcg gcatctgcat atcgcagaga
     3541 atcatatcga ccatgagcgg gttatcgcct gatttaatcg cgtggatgcc cttttcacca
     3601 tcctccgcct cgaccacatc atagccagcc ttggtcagga ccatcttgac ggtcgtcctg
     3661 atatgcgctt catcatcaac cacgagaatt ctgcccagtc ccatagtccc ctccttgaag
     3721 gttgtgatta tgttgagcca gcgtaaactt cagccatcct gcggcatcag tttgagtttg
     3781 acaagaattt agtcgaaata tgtcagaggg cctggtcgag aacggaatta gtggaagggt
     3841 gggccgtcca gaaagtttac gatttttcca cagtcgcccc gcagccggtg caacgggtgt
     3901 gcgatagaat ttcgttcccg tccgacgcga tcatcactgg tgccgggggc ggaccgagaa
     3961 gcaggcgcgc gaaggcgctc gtccgggatg tcgtgaggag cttcggcatc gccaggaaga
     4021 acagcaccat cgctacaccg gctgtgatca acggccactg agccgtcaca acgctgctgc
     4081 cgagatagac gaaggcgaac gtcggcggcg ccatgccgaa cagggtcgcg agcgagaagc
     4141 tcttgagcga gatgttcgtc aggcctgctc cgtaactcac gacgtcgaac gaaaagatcg
     4201 ggatgaaccg cgcgagaaac atggcgccca tcagctggtg atcggtgcac atctggcaga
     4261 acgtcgtctc ggtcttcatg aagcgggaga gggcatcgcg tcccaaggcc cgcgcgacca
     4321 gaaaacaggc aatcgcgccg atttccgcac cgatcaccgc atagagcgcc ccatagaacg
     4381 gaccgaaggc agccccggcc gcgaggtcca gcggcaaact cggaatcggt gggatgatca
     4441 cggcgcagac catgctcccg attagcagaa acggcgccca cggaccggcg ttgttcagcc
     4501 actgtgcgag tgcggtcggc gtgagatagc gctcataatc gatcgacaat aggccccacc
     4561 agccagccgc gactgccagg atcccgacaa tgactttgat ccaggtggtc atcgtccttg
     4621 gcaatcctgt tgcagtgagc gcggcaatac tcatggcgaa atcacttgca acacgtggcc
     4681 atccggatca cgaacaagaa aacccttctt gaatcccaaa tttccatcgg cgatcgacgc
     4741 gacgcctgat gacacgaact gcgccttgcc tcgcaaggtc gccgccgccg cttcgatatc
     4801 ggagaccatc atcgtcgtct gccagtgcca gagatcggtc gggtgcgaat cggtcgggta
     4861 gggttttccc gctgtcggca gttcgtattc gaggaactcg acgccgggag gtcccatctt
     4921 cgtagccagg ccggtcacgc gcgcgcgggc accgggaagg ctgtcgagat gttcctgttc
     4981 cgtgcccatg ttcaatgtgc cgcctgccat cgtcaggccg aggaggtcgc ggtagaactt
     5041 gatgctgctc tccgtgttcc gtaccgtcat ggccgtgtgg tcgataccaa gaaacagatt
     5101 ggaacctggt tctttccaaa caggattgcc cttgccgtcc gggaaccaga gcaattccag
     5161 attgtgtccg tccggatcgc ggaacttgat ggccttgata ccggctgcag cttggttcga
     5221 aatcggaatc gtctgagggc gcggtgaaat ctggcggacg tgatgctgac gcagtctcgc
     5281 ccaggctgcg tccatgtcgc gcacgacgat cgcaaagtgc tggaaccaga ggtcgttgct
     5341 gtatgacggc acgggaatcg gtcggacgtc aggcggtgac agaaattcga tcagctcaag
     5401 ctgttgcccg ccaagctgca tccggaccac acgggcccgc acgccgaaga cgccccagag
     5461 tcggtcgtac tccggcccat ccacctctac gtcgctgact ggcttgaacg tcagcacatc
     5521 gcggtagaag gcgatcgacc ggtccatgtc ggagacggta aagccgaccg aggcgacgga
     5581 ttgaacaacg acgggcggcg ctgcagccgc agtgaaaggt gaacggtaag gggtgagcag
     5641 tgagaggctg acaagggtta ggattcggag cagcttcaat cccatcacct cacccctcac
     5701 tccttacccc taaccgttgt aagacgtggt ctcctactct cagggcattt gcaatgatcg
     5761 tcagcgaggg attcaccgct gagctcgaca cgaagaagct tgcatcgacg acgtagagat
     5821 tgtccagatc gtgcgccttg cagtgtatgt cgagcacgga cgtcttcgga tcgcgcccga
     5881 aacgaaccgt tccgcactgg tgcgctgttc cggcgatagg aatcttcttc ccgagataga
     5941 gatgcgtcgg cagcagatgg tcctcgcagc cgatgtggtt gagcatgctc ttcagcttgg
     6001 ctgcaaggcg ctgatgcgct tccagattgt tttccgtata agcgagggtg atgccgccgt
     6061 ccttgccgac gagcacgcga ttgttgggat caggcaggtc ttccgaggtc atccagaaat
     6121 cgagcgaatg cttcgccatt tgatcgagcg cgaagccggg ggcaaaagcc ggcgctcccg
     6181 cccgcagcgt atccatgtcc gatttgccga tcatcgagat gtgccccatg gggaaatccc
     6241 agtcggcaga ggcatggtag aaatcgttca agccgatcgt tttttgaaag accgtgggat
     6301 tgggcgtttt cgagatcgcg agcatggccg aattgttgtg gcacatgtag tggcggccaa
     6361 ccatgtcgga agaattcgcc aggccgttcg gatgtttgtc gttcgcggaa cgtaagagga
     6421 gtgccgcgga attgatcgcg ccggccgaca ccaccacgat gctgctcttg tatgtttctt
     6481 tctgtccgtt ccgttcgacg atgacaccgg tcacctctcg tcccgtgcca ctggtctcga
     6541 gcctcgtgac gagcgcgtgc gtgatcatcg tgacgttcgg atactgcaac gccggatcga
     6601 cacagaccac ttgcgagtcg gccttggcgt tcaccagaca aggatagccg tcgcaggtgt
     6661 tgcagcggat gcaggcgctt tgttccggcc gctgctcgtt cagcatgatg ccgaccggca
     6721 ggtgaaaggg cttgtaacca catttctgcc agtcctcgtg cagttcctgt atccgtggtt
     6781 cgtgggtgag ggctggatac ctgtagggcg cgctggcttt cggttcggtc gggtcctcac
     6841 cgtgattgcc gtgcacgtga tacaggtgct cagcctgggt gtagtaaggt tcaaggtcgt
     6901 cgtagcggat cggccattcc ggtgagatgc cgccgtgatg cttcacctcg ccgaagtcct
     6961 gcgctcgcat gcgcagcagc gccgcaccgt agactttgga attgccgccg acgttgtagt
     7021 ggatgccggg gtgaaacgtc ccgccgtcct tgtccttcca ggtttctttg gccttgtacc
     7081 ggttttcgat gaagacggtc ttggtattcc agttgtcttt ctcgcgcggc aggtagccgc
     7141 cacgttccag gaggaggatc ttctttccag atggggcgag cttgtaggcg agggtgccgc
     7201 cgcctgggcc tgtgccaata ataatgatgt cgtagtgaga tgccatggcg gtaggtcgat
     7261 tgaggatgag aggtcttaca gaatgacaga agagattagg atggcaggac ttctctgctc
     7321 cagtgctgtg actcgaccac actaagtttc tcatctgctc gatgccgaaa ctcattccgt
     7381 tccgagcttc gcttgaacgg aacatcggac atcggcatcg ccagcccaag gctggtcgca
     7441 tctgttcaac aagtgtggtg cccgagcctt cgcacaagtc gcaggatagc cctgccatcc
     7501 tcaccccgac ggagagggga gaaactagca agcttggagg gatcattagt atggcgggcc
     7561 tgctctgctc cggcgggggg taaaaccgca tggcctcgtc gccgctcgag aagacaacgg
     7621 tgtcatgaac tgattcgcgg cgctcggcgt cccgccgctt gattgacggg acttccgcag
     7681 atcgctccgt tgacacctgt ttcatgtgga cgtagtcttt tccccatatc gggaggcgcc
     7741 atcgttggaa gacgagcatg atcaagagag tgtagatacc cgtaccgctg ctgccgctgt
     7801 cgttccttcc gcatgcgacg cgaagccgat ttactttccg gtgtctttga ccaaatttgt
     7861 ggtcatgaat ttctgtactt tcgggatgta tcagttttat tggttctacg agaactggaa
     7921 gttcatccgg gaacgtgaac agtcggatgt cagtccattc tggagaacgg catttgcgtt
     7981 catctactgc tacccgctgt tcgaaaagat ttggagtagc gccgcttcgc tcgagatcgg
     8041 tcaacctttc tctgtcccat ttctagcaac cggttggatt ctgagttcgc ttctcggggt
     8101 gctcccagat ccatacgcga tggctgcttt cttgtccttt ctcttcctta tcccggttca
     8161 acagactgcg aaccgaatca acgaatcact ggcccccgga catgatcgga atgaccggtt
     8221 gacagcctgg aacacggttg ccgttgtcat tgcggctttg ctgttcattc tggttgtcat
     8281 cgcaatcatc atctcacgtg agtaggagca ggcttacagg tccatttctc tgcgcgcagc
     8341 atcccaccgc ttgaaaggcc ggttttcttg atacatccgg ttgacacttc tctcgctaga
     8401 acgtagcctc tccccatttc agcctttgaa ccagatcgtt cacgaggacc tacgtttggc
     8461 catggattgg tcggtgttaa tcggtttgct cgctcttgtg attgtcggcg cgaccgcctc
     8521 attctatgtg catgcgttcg ttgtccggac gaggaatgcg ttccgcaatc gtcaacccaa
     8581 tctgcgaatt accaatctgt ctgcgttgaa ctcaggggac gtcctcaccc tgtgtccgga
     8641 acttgagaac gtaggcggcg gggtggcgta tgactgtgtg atgcacttgg gcggttggga
     8701 agggagtttc gccgtcaaga gccttcaccc gcgcggcccg cggtatcaga agcatctgat
     8761 ctccatcgtg ttggggccgg acgcgccgat ccgcgtgaaa cccatgacca atgactttct
     8821 ccggctccgc tatcgagatc gctgggggtt cacgtacgaa tgttggtacc atgtcgccca
     8881 aatcagaagc ggatcgatgc ggttccacaa tgtccacatc gacttggagc atccggacgt
     8941 cactgagcct catccatcgt tctgggagat gtggaaactg cttcggacca gttcccccac
     9001 gtgactgagc aggacaagcc ttcagccccc tttcgcggcg cgcggcgtcc ccttggaaaa
     9061 atacaattcc gttgaccctt ctgcggtttg gatttcaaat acagctatcg tcatcgttgt
     9121 cggaccggtc catcgcttgc acaggctgga gggtgccatg aaccgttatt atgtggtcat
     9181 tgttctcgtc ggtctcctca ctcttgtcgg ctgtatctct catccgcaga ccgccgagga
     9241 gtttcgcaaa gccgttcccg gggcgttttc agccaaagtc gagacgtttg aggtcgatcg
     9301 ccccttcagc caggtggcca gcaccttcca aaagatggga ccggactgtt tgtcgaagac
     9361 gatcaagacg acgtcccaga ccaacaggag ctatcaggtc attgtgacga cctacaaacc
     9421 cacagtgttg gtgacgggga agaaagccga gttgcacgtg cagcagcacc atgaacaggg
     9481 cgtgctcaat gtcagcaagg aacctgacgg gggctattat cttctggtcg ccgatgccca
     9541 acctgtcacg caaaccaaga ctcgcatcga tctttatcgc cagtccatcg gtcatggcgt
     9601 cctgatccag gctgtgaaga attgggcgac cggcgagaat gtcggatgtc ccgatctgac
     9661 gaagtgacgc cgctcgccag aaaccgagca gcggggaacc ctctcggcac cgtcaggatc
     9721 gccttcgtcg cattctcgat ctgtactgca agcctccttg ccggctgctt cggaagcaag
     9781 acatacccga acacgctcga cagaaacctg cacgtgcaca ctgcaacgga ttcagggtcg
     9841 tggttctcac accttcgcgc ggccgtcgac atccatcgtg tgggcgagga ttgcgccctc
     9901 ggctatgaag gcacggtcca gctgactgac ccgaagattg atatcggaat cccgccccac
     9961 cgatggagtt atttggtgtt cgtctttgac agttcctcgt tcttgggaaa ccggagcggc
    10021 accatcacat acgagacgct gctgaagcct cgctcaaatt atcactatga tgtcgcggtg
    10081 aggtacaggg acgagatgta tcacgttgag attcgggaga ctcctccaga tcaatccgcc
    10141 ggccgtgaac ttgatcgatt ggcattcagt gcctgtcgat cgtcctccgg ccggaagtag
    10201 gcatgcgatg ccggtccccg tttcgcggcg cgcggaaggt ttaccgggct aggtggatgg
    10261 aagcgatggg aatgttaccg tgcaacgggc cggaaggaga gatccttccg gcccgtcatg
    10321 tgtgcagaac agctgcgtga ggtcctattt gttgaagcgc accacgatcg cccccattaa
    10381 atcgcccttc ttgaaatctt tcttcgggct catcggatgg gcgttatggc aagacacgca
    10441 ggagtccgcg atagcgaggt cggctgcgat tcccttaaat tgcttgccgt cttcaaacgt
    10501 cagaactcct gtcgcgggat tcgcagccag ttttttcagc gcctcttctt ccgctttact
    10561 cttcgggaga ttctctttgt aaatcggcgt caatccgatg aggctgagct cgaagtcttt
    10621 cagttccgtt ccagccgcct tgaggaactg agctgggagc atgatcgaat gatcgtcctt
    10681 cgtccagttc tcatccggct tgacgttcga ttttgccgct tgcgcaacga cctgttttgc
    10741 atacacggtc cggaacgctt tcgccgtagc gagaatatag cgggctgcgg agtcacgtga
    10801 gaactcttcg gcgaatacca tcgggatcgc gactccgatt gccaacaccg acacgagcac
    10861 gcttgtagta acgctgaccc tttgcctcat gcgccacctc ctcgttaacg taagggttga
    10921 ccgaggggac gagatttcct ttaacggtgc gcctctccgc ttgcccttgt caagcgcgct
    10981 gcggggagcc tgcgtgaaga ttgtgtggtg agacttaacg agctagatgg attcctgggg
    11041 gcggacgcgc gcgctcatgg caggggggcc cgaggctccg ccacgcacgc accaccaggc
    11101 atgggcctgg tttttccgtt gcgaggtgac atctcctcga agaaagtcaa tggcataggc
    11161 gttggtcgga tcagcgtctt gtgagctggt ggtccaatag gggtccgctc tcaccccgcg
    11221 gaatggatgg ctggccggca gactgggctt ggtcgcggtg gcgagtggag ccggctcgac
    11281 gagagtcatc agctcgagga acgacggtaa gcgccacccg agtcgtcctc cggttgatgt
    11341 cagggcacag cgaagcgagg cgtttgccca cgccgtctcg ccagagctgg gagttcgttc
    11401 ccatatcaag tgagtttcag tgtcgagcac ggcttctccc ttgtactctt caaggatctg
    11461 gaatcttgac ccttgcacct gcacatcagt cctcggatct gaagggagcc ccacagcagg
    11521 gatgctcaga agtgatatgc cagccagaat ccccatgatt cttcgtgtca ggcttgggac
    11581 tgtatccggc gacataacag cggcctcgct tctgaatcgt tagtggttcg ctctcgtcat
    11641 cgtcagcaaa acccggaccc cgcacgatga tccgatggag ccggagagga ggtccgagag
    11701 cagactcgtt caatgcaaca gaggtgggga ttttcgtgcg gaagatcaga ggtttgatta
    11761 aatgatgacg gctcgtcggg atgaaggagt gccgcctgac cgagagacgg gcacacctcg
    11821 ccccgctcct tcgctcgttc caaattgaac tgggacaagc cactttgcgg cgatctgttt
    11881 ccatctatcg acattccctc gcgtgcccgt tgacaccttt ctctccgaga cgtagcctgc
    11941 ccgtcattcc atcctatggc tcgcaagcgg atcgtgatta tcggaggtgg gtttgcaggc
    12001 ctggcggccg cccgatctct gcgccatgcc gacgtcgtgc tcatcgaccg cacgaatcat
    12061 cacctgttcc aaccgctgct ctaccaggtt gccaccgccg cgttgtctcc gagcgatatc
    12121 gcctggccgt tgcgtacggt cttccgctcg caatccaata tccgtgtcgt gatggacgag
    12181 gtgctgtcga ttgatcggcc ggctcggctg gttcgtttac gggacagcgc gccgatggcc
    12241 ttcgacgttc tcatcgtcgc tccaggctcg cgccacgcct acttcgggca cgacgagtgg
    12301 gagccgtttg cgccggggct caagacgatg accgacgccg tcaggctgcg cgagaagatg
    12361 ttgctcgcgt ttgaggacgc cgaacgacgg agagccgcga ccggcatgca ggatcgactc
    12421 accttcgtga tcgtcggcgg aggtcccacc ggggtggaat tggccggcgc gctggccgag
    12481 atcgggcgaa aggccatggg gccggatttc cccaccttac gactcgatga tctctccatc
    12541 gtgctcgtgg aagggggccc tcgcattctg cccgggttca gcgaggacct ttcggtgaaa
    12601 gcctcggccg cgttgacgca catgggagtg acaatcaagc tgaacagccc ggtgagcgca
    12661 gtccgcgccg acggcgtgat gatcggaaac gaattggtcg cgtccacgaa tatcatctgg
    12721 gcggccggga acagggcctc gccactcctg aacacgctct cggttcccca ggatgcatcg
    12781 ggccgggtca aggtccggtc ggatctgaca attccgggag atccctggat ctttgtcatc
    12841 ggcgatgccg cacattgtgt cggggtcgat gggacgccgt tgccggggtt ggctcctgtc
    12901 gccatgcagc agggccgcta tgtcgccaag ctgatcgccc atgacacggc tccggagcag
    12961 cggcgaccgt ttgtctatgc gggccgcggc atgctggcca cgattggccg cgcacaagcg
    13021 gtggcgcagc tcgggccggt ccacgtttcc ggactcttcg cctggctgct ctggtgcgtc
    13081 gtgcacattt tcttcctgat cggcttccgg aatcgggtgc gagtgatgtc ggaatggatg
    13141 tggtactaca tcaccttcaa gccgggagcg agattgctct ttgaacgacc gcgagagccc
    13201 cgcaacggac cgatgccgag agaccattca cacgaatgag ccggatccgg gaacgcatgc
    13261 gttacgagaa cgccagttgc ggcggtgtcg actttcgctt tcatttacgc tgcaggtgcg
    13321 gtgatatgaa gaggtcccgg aaccgtcttt tcgcttcatc atggtggatg tcggctggtc
    13381 tgtttttagg gacggtctct caccgatgcc atgagagacc gtctcttccg atcacaggtc
    13441 tgagggcttc ttgtccttta ttgtggccca attgacatca ccttttttga tgtatccagg
    13501 gatgtctttc tcccatttct gctggatgct cttgtccagg tttaggtaga gtttcccctc
    13561 gacgattctc cagacctcag ggtctgccac gaatttcttc ccaacgccaa caccataggc
    13621 gcagtagcct ccataggccg gcagatattt ctgcgggttc gcctcgaaca tcttttggtg
    13681 ttcggcattc gcgaaggcgt acgtcacacc gtcgacgacc gtgacgtgaa atccgctccc
    13741 ccgaatcggc ttgccatcgg taaaataagc caccgggtca tacccactaa tccccggcgt
    13801 gctgtggcta aagtcctgag cgaaggctga agccgaggcc atgctcgcga cgagcaccat
    13861 gatggagacc aagaacttgc ttgcgattgt catgacatct ccccctttat tgtgtagttc
    13921 ctccgtcgga cgcctgctgt cggatcgtca acggaccctt caactcaatg gcgatccttg
    13981 tgaaggtctc acgcatcgcc tgtcgccccc tttctccgtt cctccgggtc cttgaagtcc
    14041 aggtagacaa ccctgcctgt cgcttggtcg tgccaggact ccgcttctta cagactgacg
    14101 ataaatactt ggaccgtgtc agaacggcgg tgctgctcgc cgaatctcac actctcagat
    14161 tccctatcgg ctcagggcga aatttgaagt tgcctggcta gtggcacgtg gccgaaccct
    14221 gcccgctcca atcgagcgac ttcgctcaaa acgcacggta tgggattgtc actattcgat
    14281 ggttggtggc gttcggcatc cacgcttgtg tcggatgttc tcccaatcct gttgttttgt
    14341 tagatcctct cccgcgccgt cccggtgatc ctcatgattc ctttaaggga atacaacttg
    14401 cttatcccct tgctccaaca agtccgtaga aaatccaatc cgattgcagg tcgaagcatt
    14461 catcgcgagc aaggaggcgc tacgtaatcg tgggacaagg catggttact gatcagattc
    14521 tcgagtttgt gaaggctcat cccaactgca cattggacga ggtcacgcta cgtctgcagg
    14581 aatggaattg gtccgaggtc tttatagagg ttgaccgcct gaatcggtta ggccggttgc
    14641 gtctgatcag gagtggcacc ggatttgtga cgaggctctc tgctctctaa gcgaccggtg
    14701 cgtcattggt tcggtccaag cgcgatggga atggacaagg gtggaggttc tggcagtagt
    14761 tgcgcctgct gaccacgaag cgatctcttc ttccctctgg ggagacctcc accccattcc
    14821 ctcttagccc attctttttc cgaagcgacc agcctgaaca gcctggacat cgtaagcgtc
    14881 aagacggttt gcttcctctc cttcccaacc tggccggagc tgtcgaccct ggagcattcg
    14941 gcaagatctt caagagacac tgaagtcggc ggattccctt gacacaggga ttcagtgcgg
    15001 tactgttgct ctaaggtgag cggattcatt ggaggtttcc tctaatcgac ctcaccgtat
    15061 cctcaaggca gtcgtctggg acgccggcca cttagagcaa tccttgatcg atcagacacc
    15121 tattctcatc gtcgacgatt cgccggacga tctcgtcctc ctcgatcaga tgctccgtgc
    15181 gcatggctat tccacgattc ccgcttcgtc gggacaagaa gcactcaaga agatcgaatc
    15241 gcacagtcct caactcgccc tgctcgacat catgttgccg gacatgaatg gatacgaggt
    15301 ctgcaaactc atccgacagg atccccggac ggccctgctc ccggtcatta tggtgaccgc
    15361 cctgcgcgaa gatcgaatca ccggcatcga agcgggagca gatgactttc tggtgaaacc
    15421 gatcaataga gatgaattga tcgctcgtgt tcgctcgctt ctaaggatta agtctctgca
    15481 tgacgctgtg aagggacatg tcggtgaact tgccgagtgg aacaagaaac tggagatccg
    15541 actggcgcag gaagctaagt tggccgaagt gggccgggtg ctcggcgaca tttcccacga
    15601 gatcaagaat ttactgatgc cgatcgtcac agggacgggt ctgctgcacg aggaactgaa
    15661 agatatcttc gcccttatgc cgcagaaggg cgcggacaag ctgcgggcga gcgaaaaaca
    15721 atgcgctgag atcgaggaga tgctcctccg ggccgcgaac cgtctccatg aacacgtcca
    15781 ggagattgcg gattgcgtga ggaatatcag ttctcccccg gcgtttgccc cgtgctccat
    15841 cgcgagcgtg gcggacggcg tcttcaaaac cctgcgtgtc gttgcggaag ggaaggacgt
    15901 ttcgttgtgt ctcgaccacc tgaccgatct cccccccatt caagccgacg agcgacgcat
    15961 gtttaacgtc ttctacaatt tgattaataa cgcgctgtcg gaggttcccc ccggaggctc
    16021 cattacgata cgagctgcgc acgagcctga ctccggaacg gtctgcatct ccgtgaaaga
    16081 caccggtccc gggatgccgc cggagattcg cgaccgcctg tttcgggaag gcgtcatcag
    16141 tcggaaggcg ggaggcacag gattggggac caagattgtg aaagatgtga tcgatgcgca
    16201 cggcggtcga atccgcgtgg aaagcgaact cggacacgga acaaccttct tcatcaccct
    16261 tccgatcgaa caagtcgcaa gaactgcctc attcgattaa ggtcgcgacc attcaggcgc
    16321 ggattcctcc ccctctctcc ggcaattctt cttcgtcccc attcccatgc cgcggcgttc
    16381 tgcctctgaa ggctgacact ttcttcttca agacgcattc tgtttcattc ttcaatttgt
    16441 accatgtaag aaacacatcc tttgaatgac aactgctcca agaggctgcc tatggggttt
    16501 tctttgtgtg ttcttgtctt gttgatgctt tcggggtgcg gcggctttcg aggggggatc
    16561 gagagcgttc cgtatgtggg tgacgatgag ccgcagcaga cttcgactaa ccgttcttgg
    16621 ccacatgaaa tcaaccttcc cggcctgacc ttgtacctct ccttaaataa tactgttcaa
    16681 agctatcagt atgaagtcat gctctatatc atccccactt atctgaactt ctgggatgag
    16741 ttccagcatg gggatgccga aaacgtggag cttacgcttc aaatgacccc gcacgaggca
    16801 agtatatcga ttgatccccg gaagctcgtg cttactgtgg acgacagaga atttcgtcca
    16861 atagccgtgt gggtcaataa tctggaacga gagcgtcagg tcattgatgc ctatgtcaaa
    16921 gcccgtcgcc aggcacctcc ggatcagcca ccctcgattc cgagttcctc tgaatggcgt
    16981 gattccgtca tggcaccagt gataattcgc ccacgagaaa agtcccctcg atttatcgtc
    17041 acgtttcctg ctcagcttcc atcgccagaa cgaacgctat cattgaattt gggcacagcg
    17101 atcatcgagc cattgtcctt tgatatccca cttatccgct tcaaaccgat gcactggtct
    17161 gaaggatata gttgattgct ccttgggccg gcacaaagat gggaggattg cataggagta
    17221 ggtatgcgga cgaagctccg tttcaaggca tgctgtgtcc attactgaat cgccgaacag
    17281 tttgggcgca agaatcggat agtctgccag gcagcgttgg cggtattatg agctctcttt
    17341 tgtgagggag aagatcgaat gccgtcagtc gatacaggtt ctgcatggga cgcagtgctg
    17401 aagcacgact gccggttcga tgggtccttc gtctacgcgg tgttctccac gcggatatat
    17461 tgtcgccctt cctgcccctc acgccgaccg acccgccgac aggtcacgtt cttcaaatca
    17521 ccacaagagg cggaagcggc aggatttcgc gcctgcttgc gttgccgacc gcagtcgacg
    17581 gctggttcag agattgaaac gcgggtggta ggactccgcc gctatttgga tgaacacgct
    17641 gatgagccgg tcacgcttca gcagttggcc aaggaagcag gcgtgagtcc ctttcatttg
    17701 cagcgaacat tcacacggat cgtcgggctc tctccaaagg cctaccaaga tgcgcggaga
    17761 atggaacgat tcacgttgtc gctgaagcag ggggccaccg tgacacatgc cacctacgag
    17821 gccgggttcg ggtccagcag ccgtctgtat gagcgagtga atgcgacatt gggcatgacc
    17881 ccatcggcgc ttcgatcggg cggaaccggc gtcaccttgc gctatgcgac cgtcgccacc
    17941 ccagtcgggc gattgctcgt agcgatcacg gaacggggtg ttgcggcggt gagtctcggc
    18001 gacaccgagt cgtggttggt cgcctcgctt cgacaggagt ttccgaaggc gaggcttcgc
    18061 aaggacccag acggattacg accgtatctc caggcgatgc tgcagtgcat gaccggatat
    18121 ggtgacctcg cgcagctctc gctggacgtg aacgcgacag cctttcaacg gaaggtgtgg
    18181 caggcgctgc aacggattcc tcgcggagag acgagatcgt atcaagagat tgcacgcacg
    18241 attgggcagc cgacggcgtc gcgcgccgtg gccagagcct gcgcgaagaa tcgtattgcg
    18301 gttgccatcc cttgccaccg cgcgatacgt ggaaccggcc aacttgccgg ctatcgctgg
    18361 ggccttgagc gaaagaagca gttgctcgac ttggaacgtg cgtgagcgag agaacattcc
    18421 gctcggcgtc aatgtttccg ttcgtggctg gccctcttga agccaggacg gcaggagcat
    18481 acagtgaagg cattctcggg gcattgcgta tgcgtgccat cttcgtgatt attgtggcct
    18541 gtcttgcggc gggttgtctg tcgacgactc gtgagcgcga agtccattgt cttagtgcca
    18601 tgatgatgga tacgtggcag gcgaagaatg atgtcgagtc cgctgaacgt gcctggcgga
    18661 ctgcgcaaca agctcgcttt gaacggtcgc gtgcccatcg ggattcctcc gtgctatccg
    18721 tcctgatcga caacaggtat ccctcctccg gcaccgtcgc atcggactct ctgcaaggcg
    18781 atggagctgg caaccggccg ccggaaattg atgaggagcg aaccctgtat catcgcgtcg
    18841 cggccgcgca ggcccgacat cgcgagacag tggtgtggta cggtcgtgtg gctcatcgtg
    18901 ttcagaccag gatcgaggaa gacgacatgc tctatccggt gttggggatg ttggcgacgt
    18961 cgacagggat tgtgctctat ccgctgatac ggtggaacgt acgctcggtg ctctgggagg
    19021 gcgtcgatcc cgacgcggat gacgatcctg tgcaggtcta ttgcgcggcc cgactgggac
    19081 atgagaatcc tggcccccct ccgtaaacgc gatccagcat catcagaggt cgggagtctt
    19141 atctgctcgc gcagaggtct ggcttcattc aatcacaggg acttagggga aggggagtgc
    19201 acggtccgct tcctgtaccg ccgcgacagg ctctgtgaaa ctgtcgcctg gtccgttcgc
    19261 tggatgactc ggtcatgaaa acattgggac tggctgtact ggcggcgatc ggcgggtatc
    19321 tgatcggatt attcggcggg atgctgctgg tcgagacctt ttcttcgaat cagcacgaca
    19381 agtcggtcga ggctgcgatg accggggcct tcgttgtggg gccgctgatg gcagtcgtcg
    19441 ccgtaattgt gctattgatc ttccggtccc gtcgtgcgca gtagtctatc gttgttgtcc
    19501 caggagaacc tgtcgccatg attcacatgc ttcaacgaat tgttggttcc gtcgccgttc
    19561 gtgatttgct tggaaatgcg gtactggggc tcatattgtt gggcctcctt ccttctccgc
    19621 tcgatgcggc gcggggaggg ggcgggaaga cggaggtgct gccggtggag aaggccgcgc
    19681 ctgcggtggt tgtggttgaa gcaagcggca gcgaacagga actccgggct caattacgca
    19741 aaaagagtgg tgtagtcgaa ctgaacgtgc cccagtcgaa gttttccgaa gctcctaccg
    19801 ggagtttcgg ctttattgca ccacagttcc tcggcatggc cttggtgacc caaagtccag
    19861 atttgcttat caacaaagtg gctccgaccg ccaacgccta cgaaattcac aaattagccg
    19921 atggtagcgg attgctgatc ggatttattg caaaagatct cgctccacaa gtaacgccgg
    19981 caagccggcc ccatcatgtc cgtatcgccc tttactcaac cccttcagac aaggccccgc
    20041 tcattgcggc gttgccgatg atcaagctca tggtcgatcg tctacccgtc aagcttgatc
    20101 cgaagaaggc cgatggtcct gtcatgctcg acatggactt gcaaggcacg gcgaaccgca
    20161 agtcggtgcc aggcacacaa tagctctgcg gggctgggag ccttcccccg tgccgattct
    20221 cgctcgcttg actaagtccc agagcgggag cgatgctaaa cgatgtcccg gaggtgttat
    20281 gtgaaaccgg ggtagcactt ctctgcggtt cacgggtcac tacgagtttc tctcttctac
    20341 gtctgtgcgc ccttgccgtc ctcatgcctg cgagggcgct cttgtttttc ccctctcaca
    20401 tcaaggaggt tgcaccatga agacctcact catgctcttc attgctctgc tgtggatgtc
    20461 tgcgcctgcg tttgcgaatc cggacatgct cccgaaacat ccgggttatc ccatgggcaa
    20521 ggctgtggac ccggtcaaag gacaaccgct tgcgaatgat ccaggccagg gtaacgcgac
    20581 gggagaaggt tccctgagca aggcagcggc gtttgatgat gcccacgtga aa
//
LOCUS       DATIFR010000103         5718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_451316, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000103 DATIFR010000000
VERSION     DATIFR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451316"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1917)
                     /gene="mnmG"
                     /locus_tag="VLD60_05670"
     CDS             complement(19..1917)
                     /gene="mnmG"
                     /locus_tag="VLD60_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HSB44479.1"
                     /translation="MSQSFEVIVVGGGHAGCEAALAAARMGASTLLLTMDRERVAQMS
                     CNPAIGGIAKGHLVKEIDALGGEMARNTDRAGIQFRFINTSKGPAVRALRAQCDKKLY
                     RQAMRETLERQAGLTILQGTVDRLLTKAGAVTGVVTDSGDKIDAKAVILTSGTFLKGL
                     IHIGLNHFPAGRAGEASAEHLSDCMREFGFEVGRLKTGTPPRLDRDTIDFSVMIPQPG
                     DNPPPTFSYRTDRILVPQRECHLTHTSQATHEIIRKNLDRSPLYSGVIESVGPRYCPS
                     IEDKVVRFAEKPRHQIFIEPEGLDTPEFYPNGISTSLPVDVQATMLKTIPGLERARIL
                     KPGYAIEYDYFPPRQLHPTLETKLVEGLYHAGQINGTSGYEEAAAQGLMAGINAVLKL
                     QGNPPLVLDRSQAYIGVLIDDLITKDAREPYRMFTSRAEYRLLLRHGNADLRLTEIGR
                     HVGLISDQVYEMFSKKRHSIEREIGRLEELRPRLTDDIRRRLADANIQDISGPCTVAQ
                     LLRRQEVTYEKLLDCLDLNRSQDYEIIEEIEIQVKYSGYIKRQLQQIGRFKKLEARAI
                     PAAFPYDRIVGFSEEVREKLKRVRPASIGQASRISGVTPAAISLLLVALEKHRGEASI
                     EGVLQSSS"
     gene            complement(1932..3374)
                     /gene="mnmE"
                     /locus_tag="VLD60_05675"
     CDS             complement(1932..3374)
                     /gene="mnmE"
                     /locus_tag="VLD60_05675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HSB44480.1"
                     /translation="MIGTTMRGGLEDTICAIATPAGEGGIGIVRLSGSQAMSIAGQFV
                     RLRSGRLFTSVTTHTLHLADLLLPPSARRDAPSKSGGDPSLVDHLDEALVVYMKAPRS
                     FTGEDVVEIQTHGGGLVLALLCHVCVNAGARLAEPGEFTKRAFLNGRLDLSQAEAVLD
                     TIRAKSSASLAVAQRQLRGDVAREVESARAALLGLLVHLEAGIDFVEEDISFIEQGEL
                     MQTIERVTALLRKLRATTREGRLLREGARVVILGRPNVGKSSVMNRLLKEERAIVTAI
                     AGTTRDVIEESIDLGGLTAHLVDTAGIRETDDEVEREGVKRSRAAQQDADLQLIVIDG
                     SLPLTGDDVTLINRATGQRHVVAINKCDLPLDVTPSSLGAGVVSTAISAKSGVGIDAL
                     RETIRAQLVGSAGDAPDGIMITNVRHQTALERAESALHQARGSIMEGMAAELVAIDVR
                     AAADALGEITGAITTDEILEKIFSEFCIGK"
     gene            complement(3382..5061)
                     /gene="yidC"
                     /locus_tag="VLD60_05680"
     CDS             complement(3382..5061)
                     /gene="yidC"
                     /locus_tag="VLD60_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSB44481.1"
                     /translation="MEKRVVIFLILSLAVILGYDFVLKQLGLLPSSSSVQEPASQESV
                     APGSKAGSSSDTNGGSIQVNGPVPAPSIPVSPPAAPATSVLPTERTVTVETDLIRVGL
                     NARGGVINSWELKRYHTAAPEQKPVQLVYQGGKFRGPLSITTPNASVEKALREGLYTI
                     ETDFTTLDISHPVGHVTLQFEDAVSGLRLQKRLTFHHGSYVVDVAVTIEGLADAYEVV
                     LGTNFGIVEWGDGFIGLIGSASLVDGKVEKETPDNELERKGLVQWAALQDKYFLSVLM
                     PQNGAAVLVKKEGDKLVSSSVRMTPLGTGASSLLQLYAGPKEYDTLRSLNVGLEDTID
                     FGWFIFGSWSVVKAVAKPIFYVLRFINEYTHNFGVTIILLTMGIKMLFVPLQYKSYKS
                     MKQMQVIQPKVLAVQEKFKDDRDRLNKELIKLYRDHKVNPVGGCLPMVLQMPVFVALF
                     NILYMTIDLRQAPFIGWITDLSVQDPYYVLPIIMGVTMVIQQKITPTTMDPTQAKIML
                     ILPVFMTFLFINFPAGLVLYWLTNNVLTISQQIVTDRVLFANKPLVVETAK"
     gene            complement(5063..5305)
                     /gene="yidD"
                     /locus_tag="VLD60_05685"
     CDS             complement(5063..5305)
                     /gene="yidD"
                     /locus_tag="VLD60_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSB44482.1"
                     /translation="MRTIFLTLIAWYRYWISPWLAPACRFEPTCSDYAGQAIGRFGVI
                     RGCRLALCRLLKCHPFHPGGFDPVEEERCGSQRVMH"
     gene            complement(5307..5672)
                     /gene="rnpA"
                     /locus_tag="VLD60_05690"
     CDS             complement(5307..5672)
                     /gene="rnpA"
                     /locus_tag="VLD60_05690"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSB44483.1"
                     /translation="MSQRGGDRALFLRASRDIEYVKLHGRRISTTLFNMLVCRTDEAG
                     ISRIGIVVGKRFGIAVRRNRAKRLFRELTRQVRPVLAPGHALLIFPKREALGLPFDEL
                     KNAWLSTLRRQGVLRSSEV"
BASE COUNT         1313 a         1689 c         1440 g         1276 t
ORIGIN      
        1 ccgaactgat agttgtcgct atgaagacga ctgaagcaca ccttcaatgc tggcttcgcc
       61 tctatgcttt tcgagtgcta caagaagaag agagatcgct gcgggggtaa ctcccgatat
      121 tcgagaggcc tgccctatgg acgcaggacg aactctcttc agtttctcgc gaacctcctc
      181 agagaaacca acaattcgat cataaggaaa tgcagctgga atagccctcg cttcaagctt
      241 tttaaatcgc ccgatctgtt gaagctgtcg cttaatgtag ccggaatatt tcacttgtat
      301 ttctatttct tcaattattt cataatcttg cgacctattt agatcaaggc aatcgagcag
      361 tttctcataa gtcacttctt gacgacgtaa taactgagcc accgtgcacg ggcctgaaat
      421 atcctgaata ttggcatcgg ctaaacgcct tcgaatatcg tctgtaagcc taggcctaag
      481 ctcttcaagt ctaccaattt ctcgttcgat actgtgacgt tttttgctaa acatttcata
      541 gacttgatca gaaattagtc ccacatgccg accgatttct gtgagccgga gatctgcatt
      601 gccgtgtcgg agcaggagac gatactcggc ccttgaggta aacattcgat agggttctcg
      661 cgcgtctttc gtaatgagat cgtcaataag caccccgatg taggcttgcg accgatcgag
      721 taccaatgga ggattgccct gcaacttgag cacggcattg atgccggcca tgagaccctg
      781 agctgcggcc tcctcataac cggacgttcc gttgatctgc cctgcgtggt acaaaccctc
      841 aacgagtttt gtctctagtg tcggatgcaa ctgccgagga ggaaaatagt catactcaat
      901 tgcataaccg ggcttgagta tgcgcgcccg ctccagaccg ggaatcgtct tgagcatggt
      961 agcctgtacg tctaccggaa gactcgttga gatgccgttg gggtaaaact ccggagtatc
     1021 aagaccctcc ggttctatga agatctgatg ccgcggcttc tctgcaaatc gcacaacttt
     1081 atcctcgatc gatggacaat acctgggacc gaccgattcg atcacaccgc tgtagagggg
     1141 cgacctgtcg agattctttc ggataatctc gtgcgtggcc tgactcgtgt gcgtcaggtg
     1201 gcactcacgc tgcgggacga ggattcggtc ggtcctgtac gaaaacgtcg gtggcggatt
     1261 atctcccggc tgcgggatca tgactgagaa atcgatcgtg tcccggtcca atcgaggcgg
     1321 ggtgccggtt ttcagccgtc cgacctcaaa tccaaactca cgcatgcagt cggacaaatg
     1381 ctcagccgat gcctctcccg ctcgtccagc cggaaaatga ttaagcccga tatggataag
     1441 acccttaaga aatgtacctg atgtcagaat gacagctttc gcatcgatct tgtcaccgct
     1501 atccgtgacg acaccggtca ccgcgccggc cttcgtgagc agtcgatcga ctgtaccctg
     1561 caggatcgtg agcccggcct ggcgctccag cgtctcacgc atcgcctgac gatacagttt
     1621 cttgtcgcac tgggcccgaa gcgctctcac cgccggacct ttgctcgtgt tgatgaaccg
     1681 aaactggatg ccggctcgat cggtgttgcg agccatttca ccacctaacg catcaatttc
     1741 tttcaccaaa tggccctttg cgatgcctcc gatcgcggga ttgcaagaca tttgggctac
     1801 ccgctcacga tccatcgtca gtaagagggt gctcgctccc atccgagcgg ctgccagggc
     1861 ggcctcgcag cctgcatgac cacctccaac cacaataacc tcaaatgact gggacacgat
     1921 tctgctcctc gctatttccc gatgcagaat tcagaaaata tcttttcgag aatctcgtca
     1981 gtcgtgatcg ccccagtaat ctcacccagc gcatcagcgg ctgcccgaac atcgattgcc
     2041 accaactccg ccgccatgcc ttccatgatc gagcctcgag cctggtgcaa agccgattcg
     2101 gcgcgctcca gcgcggtttg atgccgaaca ttcgtaatca tgatcccgtc gggtgcatct
     2161 ccagcagagc ccaccaattg cgctcgaatt gtttccctta aggcgtcgat gccgacgccg
     2221 gactttgccg agatcgctgt actcaccact cctgcgccca gggaagaagg ggtcacatcc
     2281 aacgggagat cacatttatt gatggccacg acgtgcctct ggcccgtggc acgattgatc
     2341 agggtgacat catcgcctgt caacgggaga ctgccgtcaa tcacgatcaa ctgaaggtcc
     2401 gcgtcttgtt gtgccgcacg agagcgtttg accccttctc gctccacctc gtcatccgtt
     2461 tctcggatgc cggctgtatc gacaagatgc gccgtcaatc ctccgaggtc gatcgattcc
     2521 tcgatcacgt ctctcgtggt tccggcaatt gccgtcacaa tagcgcgttc ttccttcaga
     2581 aggcggttca tcacgcttga tttccccacg ttcggccttc ccaaaataac cacacgggct
     2641 ccttcccgca gcaatcgccc ctctctagtc gtggccctca gcttccgcag aagcgcggtt
     2701 accctttcta tggtctgcat caattcgccc tgttcgataa acgagatatc ttcctcaaca
     2761 aaatcgatac ccgcttcgag atggactaat agccccagca atgccgctcg agccgattca
     2821 acctctctcg ccacgtctcc ccgaagctga cgttgagcca cggcgagact ggcagaagat
     2881 ttcgcgcgta ttgtgtcgag cacggcctcg gcttgcgaga gatccagcct gccattcaga
     2941 aatgcccgct tggtaaactc acccggttcc gccagccgcg cacctgcatt cacgcaaaca
     3001 tggcacagca aggcgagcac caatccgccg ccatgagtct gaatctcgac gacatcttcc
     3061 ccggtaaagg accttggagc cttcatatac accaccagcg cctcatccag atgatccacc
     3121 aatgaaggat caccgccgga cttggatggc gcatcgcgtc gcgccgaagg gggaagcagc
     3181 agatctgcga ggtgcagggt gtgggtggtg acggaagtga acagacgtcc tgaacgaagc
     3241 ctgacgaact ggccggcaat actcatggcc tgagaaccgc tgaggcgaac aatcccgata
     3301 ccgccctcgc cggcaggagt cgcaatcgcg cagatcgtgt cctcaagccc tccgcgcatc
     3361 gtcgtcccaa tcacttatca cttatttcgc ggtttccacc accagcggtt tgttggcaaa
     3421 gagcacgcgg tccgtgacga tctgttggct gatcgtcagc acgttattgg tcaaccagta
     3481 tagaactaat ccggcgggaa aattaataaa cagaaacgtc atgaacaccg gcagaatcaa
     3541 catgatcttg gcttgcgtcg gatccatcgt cgttggcgta atcttttgtt gtatcaccat
     3601 ggtaaccccc atgatgatcg gcaagacata atacggatct tggaccgaga gatccgtaat
     3661 ccacccgata aaaggtgcct ggcgcagatc gatcgtcata tagagaatat taaacagagc
     3721 gacaaacacc ggcatctgca gcaccatcgg taggcagccg ccgaccggat tgaccttatg
     3781 atctcgatag agcttgatga gctctttgtt cagacggtcg cgatcgtcct tgaacttctc
     3841 ttgaactgca agcaccttcg gctggatgac ttgcatctgt ttcatggact tgtagctctt
     3901 atactgcaag gggacgaaaa gcattttgat gcccatcgtc aataaaataa tggtcacccc
     3961 gaagttgtgc gtatactcat tgatgaaacg cagcacgtag aagatcggct ttgcaaccgc
     4021 cttcaccaca ctccaactgc cgaagatgaa ccacccaaaa tcgatggtgt cctccaggcc
     4081 cacattcaac gagcgcaggg tgtcatattc cttgggaccc gcgtagagct gcagcaggct
     4141 ggatgcgccg gtgcccaaag gcgtcatccg tacactggat gacaccagct tatcgccttc
     4201 ctttttcacc agaacggcgg cgccgttttg gggcatcaag acagacagga aatacttgtc
     4261 ctgcagcgcc gcccactgca ctaaaccttt acgttccagc tcattgtccg gcgtctcttt
     4321 ctcgactttg ccatccacaa gcgaagcgga cccgatgagg ccgatgaacc catcgcccca
     4381 ttctacgatg ccgaagttcg tgcccaatac gacctcgtac gcatcagcca atccttcgat
     4441 cgtcaccgcc acgtctacga catagctgcc atgatgaaat gtcagtcgct tctgcagtct
     4501 caaaccggag acagcatcct cgaactgcag cgtgacatgc ccgacaggat gggaaatatc
     4561 cagtgtcgtg aaatccgtct cgatcgtgta gagcccttcg cgtagcgcct tctcaacact
     4621 cgcattcgga gtcgtaatcg ataacggccc tctgaacttg cctccctgat agaccaactg
     4681 aaccggcttc tgttcaggag ctgccgtatg ataccgcttg agctcccaac tgttgatcac
     4741 accgccgcgt gcgttgaggc caacccggat caaatccgtc tcaacagtta cagtacgctc
     4801 agtaggcaga accgacgtgg ctggagcggc gggcggggac acggggattg acggagccgg
     4861 gaccgggccg ttgacttgga tagaaccccc gttcgtgtcc gatgaggatc ccgccttgct
     4921 tccaggagca acggactctt ggctcgcggg ctcttgcacc gaagatgacg atggaagcag
     4981 ccccaattgc ttgaggacga aatcgtagcc gagaatgacc gctaatgaga ggatgagaaa
     5041 aatgacgacg cgtttttcca tatcaatgca tcaccctctg tgacccgcaa cgttcttctt
     5101 caaccggatc gaagcccccg ggatggaatg gatggcattt gagcaatcga cacaaggcaa
     5161 gacggcaacc gcggatgacg ccgaatcgcc cgatggcttg tccggcgtaa tccgaacacg
     5221 tcggctcaaa ccgacatgcc ggagccaacc acggagaaat ccagtaccgg taccaggcga
     5281 tgagagtcag aaagatcgtt cgcatgctac acctcgctgg agcgaagcac tccttgccgc
     5341 cgaagcgtcg atagccaagc gttcttgagt tcatcaaaag gaagacccaa cgcttctcgc
     5401 tttggaaata tcagcagggc atgaccggga gccagcaccg ggcggacctg ccttgttaat
     5461 tctcgaaaca gacgcttggc acgattgcgc cgaaccgcta ttccgaaccg ttttcctacg
     5521 acgatgccga tgcgactgat accggcctcg tctgttctgc aaaccagcat attgaagagg
     5581 gtcgtggaga tccgacgacc atgcagcttg acgtactcga tgtcgcggct ggctcgcaga
     5641 aagagggccc tatctcctcc gcgctgtgac atgattcggc tcatcgagtg aagtcactgt
     5701 gcgtgagccc gtccgttc
//
LOCUS       DATIFR010000104        23322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1218014, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000104 DATIFR010000000
VERSION     DATIFR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218014"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VLD60_05695"
     CDS             complement(<1..310)
                     /locus_tag="VLD60_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSB44484.1"
                     /translation="MKRLRSMRIGVIADTHGLYDPAIEDHFAEVAEILHAGDIGGQSV
                     IRRLRRIAPVIAVSGNVDNYEKSGFPRRTILRRGRTTIAVCHVLYENGQLTKEAREWL
                     D"
     gene            complement(307..1440)
                     /locus_tag="VLD60_05700"
     CDS             complement(307..1440)
                     /locus_tag="VLD60_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB44485.1"
                     /translation="MNANALCFAAEFPATGAPCHVEVTSAGLVLHFEPPSPPIHGTTV
                     AFADLAVSVGGLDHDQLVVKWGEEPSARTLYLKDPVLISAFRRAAPPELTHHVEAAAT
                     KVRDARTHRRRMWGLALGSVSAVLLFLWLGSDWLVELAVSRIPVEWEQKLGESAYRDF
                     LAQQTVIKEGPAVAAVHEMTQRLIEKIPDNPYKFEISVVKSDVVNAFALPGGYVVVFT
                     GLMKKAENGEEVAGVLSHELNHVLQRHGLDRMVKQLGLIAVVTIVLGDQQGLIGLMKQ
                     VGVELLTLKFGREQETEADITGLQLLHRAKIDPNGMIRFFERLSEKDKERVEILSTHP
                     MSEHRAARLRAEIAALPKQQPEPFEFDWKTVQESMGVHEAVRP"
     gene            complement(1544..2227)
                     /locus_tag="VLD60_05705"
     CDS             complement(1544..2227)
                     /locus_tag="VLD60_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HSB44486.1"
                     /translation="MKSEILYPGAFPMVRVDLAAGESIKAESGAMVASSPTIDIESKM
                     EGGFLGALSRKFLTGEKFFFQTLRATRGAGEVLLAPTVPGEIVVFELDGVNEYLVQKD
                     GFLAGAETIKIDRQMQSLSRGLLGGEGFFLLKMSGKGTLMVNSFGAIHKIELKPEQEY
                     IVDNSHLVAWSSTTSYNIERASSGWVASFTSGEGFVCRFRGPGVVYIQSRNPGSFGAW
                     IRQFIPVSE"
     gene            complement(2736..4112)
                     /locus_tag="VLD60_05710"
     CDS             complement(2736..4112)
                     /locus_tag="VLD60_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB44487.1"
                     /translation="MPQTKEQVRVDRALEDLSRQSASRLGMTRRNFLRTSGGLAAVLL
                     AMNSVFGRFFDVFETELFEPAAFAEQQGAPYFIFDVQTHYVGSHYDPTDGESRRKGAV
                     AKEALLGLRRRTREAGLNPRLTADRGTLDDLSWENFVKEVFLDSETSIGLISTPPGPY
                     PQEAVVPPKEMAHIRDEINRLAGSQRMLAHGLATPQLGAADLDFMAIQAETLKVDAWK
                     CYTGSCPKGFDRGWWMDDEKIAYPMLEQARRLNVKRVCVHKGLPLGPVPDYNHPKDLI
                     KAAKDFPDIDFLVYHSGFKTVAGVDEGFARSGEIPWTTEFCRMKQAEPGIHNIYMELG
                     STFGQLVTTHPGICAHLLGQIIEAFGVDHVLWGTDSIWYGTPQWQIEAFRRFQIPSRI
                     VEQRKYQELTREIKGKIFGLNAARLFGVDVMATRNAVPKDFVGRLRMSYLEEGPEPSH
                     RLYGWVAG"
     gene            complement(4635..6794)
                     /locus_tag="VLD60_05715"
     CDS             complement(4635..6794)
                     /locus_tag="VLD60_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44488.1"
                     /translation="MPASFSTVVDAQDIPEAEPVTVELSESRVPVETASPDPSPVALT
                     QSTAQVEEPPQAVGGAETVTQVPSKPEPPAQSVEVTSHWNTGEVAVQLHRPTAKKKKW
                     EKDSAEAPGIPATPDPAFNTLSEKLSEAIREWESTPVETAPPSISEPVAAPQPDTRPE
                     WMQASEQITFSRPHVAPREEWQNSSSDSLSRPLEPVSAPTVASSAVDVLFSSTERGNY
                     SPTQERLLSLKPRPRLVARLHRIRIGVQSFVGSCFSTTRSLTFLALAIAATTGIVAAL
                     GAGALGLAWIVMEEPPSALYQNLTITPPRTITDPKKNGYLLLLGFEAPSGQDPVQFGY
                     EQKAGGQDVAASQVCMGGDDAKEGSASGGATGHVVKGWFRSANPMAQLKGQGDTIKAL
                     AAREASSLARYRQWLTMPFDDWGYGQLLSPNCDHLLLAHRLFLLEGFAQDSATGLDRL
                     ETDMQAWRTALGQSKTLMMKMLAVAAVQDDAAMASGLLSRSELDGTSVTRLSKMVRPL
                     DQVELSVRWPMQSHFVWATKAVSAELKHDQANDRPWHVSLVAGMRLPIQRRANAYAEY
                     YEATNKAVAEGRYMNLPKPSGFLRTPATGLMDYLANPVEHIVGIEPLLSWDPYVMRMM
                     ETDAQLRLVGLQAWIRRGPQDGELLTRLAKAGQAYYDPFTGLPMLVNQRKGLIYSVGR
                     DGKDQEGDRTNDVAVAIPSVPSSLSEGKRSTTPSPSR"
     gene            8151..8846
                     /locus_tag="VLD60_05720"
     CDS             8151..8846
                     /locus_tag="VLD60_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44489.1"
                     /translation="MFDRLRTDEKLPGLHIEQRSKLDGDRTPWCQFPDLCIELCGPLG
                     IFRVRIVAKNLDGFPQFAHRRHLVPLLPIDMGKELEKWNTAGAGGQILLQQRFGEPII
                     LPLNSFIGERFECLSGRDQASKSQMEGRKQPPGLSILGIKGEEFFEGCGRPAIFAGIH
                     VRDRFFEKGAFLAVADDTPLINTRGCFLVRFLGGSLVGPHVINLSRSLRTQTGFPSWF
                     QPDIRFDRLWQTL"
     gene            9222..10838
                     /locus_tag="VLD60_05725"
     CDS             9222..10838
                     /locus_tag="VLD60_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSB44490.1"
                     /translation="MYKLADLLFMYFLKSNQHNRLRWALIACLLVLLPALLVPIPLLA
                     ESGGGGQGRVTLDFNEVELAVFVRFISELTGKNFVLDDAVKKAGGKITVISPTKVSPD
                     QAYSLFVSALEVSRLAVVPKGPGYQIVPMGELPPERGVFVYKLKHANATDLAAVLTNL
                     VARSQTVAQTAPGARPPIRPLTEFEAPVQVFADKPTNSIIISATKNAYSKLQSVIRDL
                     DTRRNQVFVEAVILEVTLDRLRQIGTDPLQVLAAGESGSVRAIGGLNRAPEDLSTIAQ
                     AIVGVATGGTVSVLNTVNIRAFMQFLLSLTDTNVLSTPQVLAADNQKAKIVVGENVPF
                     PTGQAQGITGGTLVTIERKDVGVTLELTPQVLENDLIRLEIKQEITAIEPTAPQTIGT
                     GTSSVPVGPTTTKRSMETTTIAKDLQTLVIGGLVRDNILLTENKIPFLGDIPILGWLF
                     RSQSRQIQKLNLLVFLTPQLVRDETEMVELNARKAKELSTFQREGRIEEPTRLKQEVL
                     EKLERPHTAPPPVPNHAEPSAVTPPPGKPD"
     gene            complement(10943..11019)
                     /locus_tag="VLD60_05730"
     tRNA            complement(10943..11019)
                     /locus_tag="VLD60_05730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10983..10985),aa:His,seq:gtg)
     gene            complement(11106..11819)
                     /gene="pyrF"
                     /locus_tag="VLD60_05735"
     CDS             complement(11106..11819)
                     /gene="pyrF"
                     /locus_tag="VLD60_05735"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSB44491.1"
                     /translation="MSKIIARDRLILALDVPSAAEADRLMDQVQDQITFVKVGLELYT
                     AAGPEVVQRMLQRGKRVFLDLKFLDIEETVRRATERVAAMGVEFLTIHANRKALAAAV
                     KGRGSSPLKLLAVTVLTNFDSHDLREMGIPYTVQELVTARALLASEMGCDGVVASGEE
                     PGVLRPKVGPRFVIVTPGVRPAGKDVDDHARATTPTQTIAAGADYLVLGRPIRAASDP
                     AAAAAAIIMEMQAAFDSRS"
     gene            11905..12180
                     /gene="rpsT"
                     /locus_tag="VLD60_05740"
     CDS             11905..12180
                     /gene="rpsT"
                     /locus_tag="VLD60_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSB44492.1"
                     /translation="MPVIHKSTIRRARQAVKRQARNRATLSAVKNLVKKVQAAVADKK
                     VDDAKTSLREATSALSKAVTKGVLKRNTASRRISRLTQHVNSLAASR"
     gene            complement(12155..13264)
                     /gene="holA"
                     /locus_tag="VLD60_05745"
     CDS             complement(12155..13264)
                     /gene="holA"
                     /locus_tag="VLD60_05745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSB44493.1"
                     /translation="MPQAVSVAQLTTQLAQGKVAPVYLLIGEEDLLRDAALSQLKQSL
                     LGDGGDDFNCDVFYGDEAEGAQIVSCASEIAVFASRRLVIVKAADKLPARQCDSLLPY
                     LKAPNESSTLIFVASKLDGRLKFTQALIQAAVAVDCAPLKDSQLVPWLKQEADRLGVR
                     IDEEAIHLLKEACGGSLYSVRREVEKVASYVPAGRIVTARDVATLRGTEPGASVFDLA
                     AAIGAKDRGRALAIVARNMDAGEAPLRILGSLAWQYRRLWKVKELMRLGGREGEAART
                     LRMDPYKVRAFVGQFPDAHLQEALRAFLEADGKLKGGSGGRPGMLLDRLILQLCDRPQ
                     AEARKAETSSPAPAGPARARTLSNVRTISATRPRN"
     gene            complement(13279..13914)
                     /gene="lptE"
                     /locus_tag="VLD60_05750"
     CDS             complement(13279..13914)
                     /gene="lptE"
                     /locus_tag="VLD60_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HSB44494.1"
                     /translation="MSRDEKVRSLTILSIYVSALCVSLALLSACGYQFRVEGAGPTIG
                     EPAAAASSTPPPRVVIQTLKNSTFEPNLESRFTNYLRHEFSSGSGAQIVPQAEAADLI
                     VSGDILSVTLPTLSFTQTTTLESRAEVVVAIKVEETRTKRVVWTQTVKGASEFYVTTD
                     LQFNRVLQNRALEQAGRFVAEDLASRFLLQLESGRLTKPVANAAPSQPDKP"
     gene            complement(13954..16389)
                     /gene="leuS"
                     /locus_tag="VLD60_05755"
     CDS             complement(13954..16389)
                     /gene="leuS"
                     /locus_tag="VLD60_05755"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSB44495.1"
                     /translation="MSKTYDHHAIETKWQAYWEEHRPFRAMDDPIKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAIKTGIHPAITT
                     AQNIATFKRQMKRVGLSYDWDREISTTDPAYYRWTQWIFLQLFKKGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYADRLLDDLSLVEWPPSTLE
                     MQKNWIGRSIGAEVEFAIAGINGRIRIFTTRPDTLFGATYMVLAPEHPLVEVVTVADR
                     RAAVTAYREAAARKSDLQRQELEKEKTGVFTGGYAVNPVNGERLPIWIADYVLMGYGT
                     GAIMAVPAHDARDWAFAKTYSLPIREVIAGGDVDKEAFVETAKGKVVNSTAPDQSFSI
                     DGLTPVDAIPKITAWLESNGSGRKAVNYKLRDWLFARQRYWGEPFPIIWVDGEARPLP
                     EEQLPLVLPETSNFKPSGSGESPLANLEDWLVTADPASGKPARRETNTMPQWAGSCWY
                     YLRFIDPKNSKQLVDPVKEQYWMPVDLYVGGSEHAVLHLLYSRFWHKVLHDIGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMMDQFGADAVRLYEMFMGPLEAMKPW
                     STRGVEGLTRFLERAWRLMVNEEGRLSSAIVSSVPSQDHQRLLHQTIKKVTDDIEQLR
                     FNTAISQMMVFTNEMTKTEQRSRSLIEPFVLLLSPFAPHLAEELWALLGHRASVSQQP
                     WPIFDPALTVSDRLTIPIQVNGRLRAKIEVGADAARDVVESLARTEAAEWLQGKEPKK
                     VVYVEKKLINFVV"
     gene            16496..17110
                     /locus_tag="VLD60_05760"
     CDS             16496..17110
                     /locus_tag="VLD60_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB44496.1"
                     /translation="MGLYSHYIFPLLMDWVMGGEEFQRLRTAVLRDAQGDALEIGFGT
                     GLNLPHYPENISSLTLVDPARLLPHRVTERASAVSFPIHTRHMTAESLPFHDHRFDTV
                     VSTWALCTIPDPAKALRDVRRVLKPDGQFLFLEHGRSDDETVAKWQDRLNPLQNVIGC
                     GCNLNRRIDQLIEQSGLHLVHLDRFQMCNVPRIAGEMYQGLARP"
     gene            complement(17123..17806)
                     /locus_tag="VLD60_05765"
     CDS             complement(17123..17806)
                     /locus_tag="VLD60_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB44497.1"
                     /translation="MTSIQLNWNEIDDVLLDMDGTLLDRHFDNYFFEEELPRRYAAMH
                     GLLFEESRDRLMAMYQSVEGELAWTDLQYWTRRVGIDVVAMHKELDHMVDFLPGAEAF
                     LRELQRRGKRITILTNAHRAGVEVKAAKTGLDRQVDRIVDAFEVGYLKMRTEYWPACQ
                     RLVGFNPKRSLYVDDDESCLDAASRFGIAEIVHSAKSSSQLPPSPTTGFASIEQLSYL
                     LNGTPLSHS"
     gene            17876..19252
                     /gene="der"
                     /locus_tag="VLD60_05770"
     CDS             17876..19252
                     /gene="der"
                     /locus_tag="VLD60_05770"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSB44498.1"
                     /translation="MPRTKAAPAPTLPLPSTQGGPIPLVAIIGRPNVGKSTLFNRILG
                     KKTAIVDDVPGVTRDRNYADANYRNRPFRLVDTGGLDPTASEGMLALIKRQSELAIAE
                     ADILILLMDGRTGLTPADHEVVRLLRGTTKPLFVAINKIDTPKVETLVADFYQLGTDQ
                     LHPISAEHGIGVSELLDEIYPLLPVLDDKQEQSQMPRIAVVGRPNVGKSTLVNAVLGE
                     DRVVVSDVPGTTRDSIDSLAVYQGRRYLFTDTAGIRRRGKIDRGIEGYSVARSLRAIG
                     RSDVAVLLLDAQEGVTEQDTKIAGVVIRQGRACMLMVNKWDLREGDSQARQQVEQDLR
                     RRFPFLSWAPVLFGAAVKPDSLRQLFPTIDEVYASFSKRIPTGQLNQFLQNILATHPL
                     PVRKGKPTKITKSAFMTQVAVQPPVFALFVGHPDNITPAYLRFLENQLRDVYGFTGTP
                     VRLLVRKK"
     gene            20099..20902
                     /locus_tag="VLD60_05775"
     CDS             20099..20902
                     /locus_tag="VLD60_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44499.1"
                     /translation="MIGIAHDTVSLQRQLLAVMVSGLATLLCTHPLSAAEPITAVTVE
                     SPAQAPAAESAPANGSSVEADPSITLRGSVWRTKAGIVFLKTPVGILTLSSKTTLKDL
                     KGSHEVAFWVHDRNSVVEIRKRSDGSLVHRYFSGPMTLGPDASKTLRRWSAEGEQIVH
                     FGTQEEKLAAYHEGDPLTVEVDESQTVIGVHDLQFDLQVSQVPPAGSQAHLLLSGTVS
                     KLKSYFVFFRTPVGVVMVNAKIGLPRVKVGQAMTLHIDNGRVTVDLAGK"
     gene            21018..21653
                     /locus_tag="VLD60_05780"
     CDS             21018..21653
                     /locus_tag="VLD60_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB44500.1"
                     /translation="MSSIPRQKPSQPQSDQDSDGFDKLYRDHVDLIYRYAHRLCGEAE
                     SAKDLVQETFLNAYRGLKGFRGDAQVSTWLYTIASRACMRMRRKRKGEPERELSLDDF
                     VPTSEGEFRLQIPMDGLTPEEALQNKELRVALDQAINKLPKKYRMTLVLRDMEGLSAK
                     EVGAIMGANERAVKSRLHRARLFVRRELSARGITQEDHQTGKQRESGGSNA"
     gene            21650..21949
                     /locus_tag="VLD60_05785"
     CDS             21650..21949
                     /locus_tag="VLD60_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB44501.1"
                     /translation="MTKKAPARKGPGQASGRRHQHRPGNCLNILRQLSAYIDDELPSD
                     ICRELRRHLGACPNCEVFITSLRQTVTLCRHSRTPQLSAKDRMALRQKILRTAQS"
     gene            21958..22347
                     /locus_tag="VLD60_05790"
     CDS             21958..22347
                     /locus_tag="VLD60_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44502.1"
                     /translation="MARLVGPLVLISSLIGITALSFALPPAPIQLEEGRAIYERHCAD
                     CHGSEGRGDGKQSISLSPRPGNLISAQTSAKADQDLLKIIANGRPRTAMEGWKDRLSE
                     DEQRAVLAYIRSLVRFTRSATPPPPTP"
     gene            22442..>23322
                     /locus_tag="VLD60_05795"
     CDS             22442..>23322
                     /locus_tag="VLD60_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44503.1"
                     /translation="MIRLFSHTRSRLIVLVLFASASITLSTAFAAPLQKKSKGLAPGA
                     SVAQRYADAVSKGDKIEVAKLDFACQYRLVAASKSGLAAYPDQADASYDACWQGFKTA
                     HEAALTRVDVGMDVLWPSNGALAFFREELYRYPASAFVMDVVGLSPPGSGLHLKTIST
                     KALPNASFHLRPNQPLISVPATLVTMTVSYQDPLTSPVTKAPGSYKWTNTVKPARRAL
                     KSIDLQWIVLSGLKKHGFPGDAAVVNLLVSTGAPVDGIPQEIIPFVTETSKALPGSLV
                     WWGPEDAPGLLVAAAARA"
BASE COUNT         4945 a         7047 c         6364 g         4966 t
ORIGIN      
        1 gatccagcca ctcccgagcc tccttcgtta attgtccgtt ctcgtacaac acgtggcaga
       61 cggcaatggt tgttctgcct cgacgaagga tcgtccgtct ggggaatccg cttttttcat
      121 aattatcaac gtttcctgaa accgcaataa ccggtgcaat ccgtcgtaaa cgtcgaatga
      181 cgctttgtcc gccaatgtca ccagcgtgca ggatctctgc cacctcggcg aaatgatctt
      241 caatggccgg atcatacagc ccgtgtgtgt cggcaatgac accgatgcgc atggatcgta
      301 gccgtttcat ggccgcaccg cctcgtgcac gcccatcgac tcctggacgg tcttccaatc
      361 gaactcgaag ggctcaggct gctgcttcgg caaggccgca atttccgctc gaaggcgcgc
      421 agcacggtgc tcgctcatcg gatgagtcga caggatctcg acgcgctcct tgtccttttc
      481 cgacaggcgc tcgaaaaatc ggatcatgcc gttgggatcg atcttcgcac gatggagcaa
      541 ctgcaggcct gtgatgtcgg cttctgtctc ttgctcacga ccgaatttca aggtcaagag
      601 ctccacgccg acttgcttca tgaggccgat caagccctgt tgatctccga gcacaatcgt
      661 cacaaccgcg atcagaccga gttgtttcac cattcgatca agaccgtgcc gctgcaggac
      721 gtgattcagt tcatggctga gcacgccggc cacttcctct ccgttttcag ctttcttcat
      781 caatccggta aagacgacga catagccgcc gggtaacgcg aatgcgttga cgacatcgct
      841 tttcaccaca gaaatttcaa acttgtaggg attgtcggga atcttctcga tgagccgttg
      901 cgtcatctca tgcaccgccg cgaccgccgg gccttctttg atcaccgtct gctgcgcgag
      961 aaaatctcga tacgccgatt cgccgagctt ctgctcccat tcgactggta tccgactcac
     1021 tgccagctcc accagccagt cggatccgag ccagagaaag agcaacaccg cgctgaccga
     1081 tcccagcgca aggccccaca tccgtcgccg atgagtgcgg gcatcccgaa ccttcgttgc
     1141 cgccgcttcg acatgatgcg tgagttccgg gggcgcggca cgccgaaatg cgctgatgag
     1201 aacgggatct ttcagataga gtgtcctggc cgagggttcc tctccccatt tcaccacgag
     1261 ttggtcatgg tccaagcctc ctacggacac ggccagatcc gcgaacgcaa ccgtggtccc
     1321 gtgtatgggg ggggagggag gctcgaaatg aagaacgagt cctgcggatg tcacctcgac
     1381 atggcagggc gcaccagtcg cgggaaattc ggcagcaaaa cagagggcgt tggcgttcat
     1441 aagggataac cagggtaacg gggtaaatag agcaaccggg gtagaggttg gggatggtac
     1501 ctgttgtccc gctagcccgc tagccctcta ccccgctaac cccttactca gagaccggaa
     1561 tgaattgcct gatccatgcg ccgaaactgc cggggttccg actttggatg tacacgacgc
     1621 cgggcccgcg gaacctgcag acgaatcctt cgccggacgt gaagctcgct acccatccgg
     1681 aggacgcgcg ttcaatgttg taggacgtgg tggacgacca ggcgacaaga tggctgttgt
     1741 ctacaatgta ttcctgctcc ggcttcaact cgattttatg gatcgcgccg aaactattca
     1801 ccatcagcgt gcccttgccg ctcattttca gcagaaaaaa accctcgccg cccaacaatc
     1861 cgcgactcag actttgcatc tgcctgtcga tcttgatcgt ctcggctccg gcgagaaaac
     1921 catccttctg cacgagatat tcgttcacgc cgtcgagttc gaagacaacg atttctccgg
     1981 gcactgtcgg ggccaacagg acttccccgg cgccccgcgt cgcgcgcaag gtttgaaaaa
     2041 agaatttctc gccggtgagg aacttacgcg ataacgcacc gagaaaaccg ccttccatct
     2101 tgctttcaat gtcgatcgtc ggcgatgacg ccaccatcgc ccctgactca gccttgatgc
     2161 tctcacccgc cgccaaatca acccgcacca tggggaaggc ccccggatat aaaatttcgc
     2221 tcttcatgtg atcaaacctc caagcttcgt aaggagttag gcgtgaggcg ttaagcgatt
     2281 cagactgaca aaggtcaaga tctcatatcg acgcttttga caccattcgc tttttgatga
     2341 cggatcaggc cactgagcat cttgttgatc cgctcaactt gtccatcgag cattgtccag
     2401 cgatcctgcg caagatatcc agtctcctcg caggctctag gtgagtatct aattcgcgga
     2461 gcgacccttg tgccatgtga agatcgttga tgaattcctt tttcgtttgc cgaccagccc
     2521 cctcggcaac gttgctggga atgccagaaa ctgcccgcct gatttgatca gtcaagctgg
     2581 atcgctcttc cctcgggaat gaatcggtta ggtggtagat ggtgacagcc aattccatcg
     2641 ccgcattcaa gagatccaat ttcttgtgcg gtttgtccat ttctaagcta tgttcttaac
     2701 cgccattccg ctttagccct aacgcctcac ggcatctatc ccgccaccca cccgtacaac
     2761 ctatgacttg gctccggccc ttcctcaaga tagctcattc gcaggcgccc cacaaaatcc
     2821 ttcggaacgg catttctggt cgccatcacg tccacgccga acagtcgtgc agcattcaac
     2881 ccgaagattt tcccctttat ctcacgtgtc agctcctgat acttgcgctg ctccacaatc
     2941 cggcttggaa tctggaaccg tcgaaaggct tcgatttgcc attgcggcgt cccgtaccag
     3001 atggagtctg tcccccagag cacatgatcg accccaaagg cttcgatgat ttggcccagc
     3061 aggtgagcgc atatccctgg gtgggtcgtg accagctgac cgaacgtcga accgagttcc
     3121 atatagatat tatggattcc cggctctgcc tgcttcattc ggcagaattc cgtcgtccag
     3181 ggaatctcac cgctacgcgc gaacccctca tcgacaccgg ctacggtttt gaaccccgag
     3241 tggtaaacca gaaaatcgat gtccggaaaa tccttggcgg ccttgatcag atctttcgga
     3301 tggttgtagt cgggcaccgg cccaagcggc agccccttgt ggacgcagac ccgtttcacg
     3361 ttcagccttc tcgcctgctc gagcatggga taggcgatct tctcatcgtc catccaccag
     3421 ccccggtcga atcctttcgg acatgaaccc gtgtaacact tccaggcatc gaccttcagc
     3481 gtctcggcct gtatcgccat gaaatccaga tctgcggcgc cgagctgcgg cgtcgccagc
     3541 ccgtgagcca acatgcgttg tgatcctgcg aggcgattga tctcgtcacg aatgtgggcc
     3601 atttccttgg gcggcacaac ggcttcttgt ggatagggcc ccggcggcgt gctgatgagg
     3661 ccgatcgacg tctcgctgtc gagaaagact tccttgacaa aattttccca agacaaatcg
     3721 tcgagcgtgc cgcgatcggc ggtcaatcga ggattcagcc ccgcctcgcg cgtgcgccgg
     3781 cggagcccga gcaaggcctc cttcgccacc gctcccttgc gccggctctc gccatccgtc
     3841 ggatcatagt gcgacccgac ataatgcgtt tgcacgtcga agatgaagta cggcgcaccc
     3901 tgctgctcgg caaaggctgc cggttcaaag agttctgtct cgaacacgtc gaagaaccgg
     3961 ccgaagacag agttcatcgc gagcagcacc gcggccaggc caccggaagt acggagaaaa
     4021 ttccggcgcg tcattcccag gcggctggcg ctctgccggc tcagatcctc caaggctcga
     4081 tccacgcgta cctgctcctt cgtctgaggc aaaggaacaa actcctcgtt ggagacatgt
     4141 tgggtgggaa tcgggcccgg tgagatgact cctctccgga aacgcgcagc ccggtcagat
     4201 gaatcgctgt gtcgcatgca aatcccttct ggtgcgagtg cgtggcaaca cgattctgtg
     4261 gaatgctgtt cgggctgtca agggactgga cgaagaggct cctgaacgcg agcgcagcga
     4321 tcgatggcgc cggatgatcc gaacgcaact acccctccca ccgttacaga catacctgca
     4381 ccttctcaat cagcatgcct gaccggtccg acggcgaaga ttgttgatcc gaatggaacc
     4441 aaggtcaagc ggaagatgta cgcgctacgc aaagacctgg tcgcgtcggt cttaggtctc
     4501 tgctcggtgt tttcatcgtg gattcctccc gctacttatt gcaacgcatg agagcgattt
     4561 gcgttgaccg tgacagcggc cagtgcgggc gcgagagtga tcagaaatcc ctaaagagga
     4621 aggaagttac ggctctaccg ggacggggat ggggtcgtgg agcgcttgcc ctccgagagg
     4681 gaagatggaa cagagggaat ggccaccgcc acatcgttcg tgcgatcgcc ttcctggtct
     4741 ttgccatctc gtccgacact atatatgaga cctttgcgtt ggttgaccag catcgggaga
     4801 ccggtaaacg gatcgtaata ggcttgcccg gccttggcca aacgggtgag caactctcca
     4861 tcttggggcc ctcgtctgat ccatgcctgc aagccgacga gacgcagctg cgcgtccgtc
     4921 tccatcatcc gcatgacgta gggatcccac gacagaagtg gctcgatccc aacgatatgc
     4981 tcgaccgggt tggccagata atccatcagc cctgtcgcgg gcgttcgcag gaaaccggac
     5041 ggcttcggca gattcatata gcggccctcc gctacggcct tgttcgtcgc ttcatagtat
     5101 tcggcatagg cattggctcg acgctggatc ggcagtcgca tccccgccac cagcgacaca
     5161 tgccatgggc gatcgttggc ctggtcatgt ttgagttccg cggaaaccgc cttggtcgcc
     5221 cagacgaaat ggctttgcat cggccagcga acggagagtt cgacctgatc gagcggtcgc
     5281 accattttac tcagccgagt tacggaggtt ccgtcgagtt ccgagcggct cagcagaccg
     5341 gacgccattg ccgcgtcatc ttgcacggcg gcgacggcca acatcttcat cattaaggtt
     5401 ttggattgcc ctaaggcagt gcgccacgct tgcatatcgg tttcgagccg gtcgagaccg
     5461 gtagccgaat cctgggcgaa tccctccaag aggaataagc gatgcgcgag taagaggtga
     5521 tcacaattcg ggctgagcaa ttgcccataa ccccaatcat cgaacggcat tgtcaaccat
     5581 tgtcggtatc gggcaaggga cgaggcttcc cgagccgcca acgctttgat cgtgtccccc
     5641 tgtcctttga gttgcgccat ggggttggcg cttctgaacc aacccttcac gacatggccg
     5701 gtcgctcccc ctgaagcgct tccttctttc gcatcatctc cgcccataca aacctgtgac
     5761 gcagccacgt cttgtccccc cgctttttgc tcatatccga attgcaccgg atcctgtccg
     5821 gacggagcct caaatcccag gagcaacaga tatccgttct tcttgggatc cgtgatcgtg
     5881 cgcggaggag tgatggtcaa attctgatag agcgcgctcg gcggctcctc catcactatc
     5941 caggcaagac ccagtgcgcc agctcccaag gccgccacga ttcctgttgt ggcagctatc
     6001 gccagggcta ggaaggtgag cgagcgggtc gtcgaaaaac aggaaccgac gaaggactgc
     6061 acgccgatcc gaattcgatg gagacgggca accaagcgcg ggcggggttt caacgacagg
     6121 agacgttcct gcgtcgggct atagtttcca cgttccgtcg aactaaagag tacgtccacc
     6181 gctgaggagg caactgtggg agcggaaact ggttcaagtg gtctcgaaag cgaatccgac
     6241 gaagagttct gccattcctc acgcggcgcc acatgtggtc gcgagaatgt gatttgctcg
     6301 ctcgcctgca tccattccgg cctcgtatcg ggttgaggag ccgcgaccgg ctccgaaatg
     6361 gaaggaggag cagtctctac aggagtcgat tcccattctc gtatcgcctc ggagagcttt
     6421 tcggacagag tgttgaacgc agggtctgga gtggcgggaa tccctggcgc ctctgccgaa
     6481 tctttctccc attttttctt cttggccgta gggcggtgca attgcaccgc cacctcgcct
     6541 gtattccaat gtgaagtcac ttcaactgac tgagccggcg gttcaggttt cgacggaacc
     6601 tgcgtcacgg tttccgcccc gccaaccgcc tgaggcggct cctcaacctg agcagtggat
     6661 tgagtcagcg cgacaggtga tggatcgggg ctcgcggtct caaccggaac cctcgattca
     6721 gataactcca ccgtgactgg ttccgcttcc ggaatgtcct gcgcgtcgac aactgttgaa
     6781 aaggaggccg gcattgcggg agaaacagaa agctgcgtcg cttcatctgc agacgagtga
     6841 gctagaaatt ccggtagatc cgcagtcctg ctttcggccg gcattgggta agaaggggtg
     6901 ctatccggga cagctgaggt cgtgacagag ttttcggcga cctggacttc ttgtctgctc
     6961 gtcaaactct ccgccgcaac ctgattgggc tggccagatg aaagagaaac ttcgtctgag
     7021 ctggttgact cagttgctgg aagtgaatcg tctgtgagaa gcgcctccga ttcaacggaa
     7081 acagagctag tcgttgtggc ctccggttca taaaccacct cagtgtgctg tgcctcagtg
     7141 aggacctcag gcgcgatctc ggatggagcc gaagggctcg gcgccgtgga tcccgccgcg
     7201 aacttccatg ctgaatcaaa gactgatgtc catgagaagg aacttgaacg aggaacctcc
     7261 gtagtcggtt gaaacggggc atcctgttcc ggcggtgcga cgctggctgg aggctcttca
     7321 actgaagacg cacggtctag agcgaacgtg gttagtatgg tgtccgccgt ctgtgctgga
     7381 agtgcttcgg atgaaagaga agacaacgga ttctctaggt cctttctgaa gtcggagagg
     7441 gtcgattccg ccgacgcggt aggagtttgt tcaggggaag actccatctc ctggacttgt
     7501 atcgacgaat cggacacttg ctcccacggc atcggagacg aaagtgcgac cgagccaccc
     7561 gccgataaag gctccgtcga tgccgacaac ggtgccggac ctgttgcttg ttccaaagca
     7621 ggcattggcg agaaaggagc tacaggaggt agctctacat tttccgaaga cgaaaggata
     7681 ggctgaaggc ttggggatga ctgaagctca gattcgggta tttggaccga ggcatctgat
     7741 acctgttccc atggcatggg ggatggcaat gaatcccctg taccagtcgc cgactctgaa
     7801 tgatcctgtg gcgggtcctg atcggcggct tcttcctttc gataggcagg ttctactttc
     7861 gagtcggtct gatccaggcc ctgagaagtt agcgccggag aaaggatcga aaattcctgc
     7921 acatcagccg caggttcatc ctcacgcacc gcattctgcg ttggtagttc ctccgctatc
     7981 cgaaactcgg cttgctcaat tggagcagca cccgatgatg ggtcctcttg tgcgtctggc
     8041 gatgattcct cgctctcctc ccatggcatg acttcggaca acgaggtctc tgcttttatg
     8101 gaagtcgaat catcttgggc catggtggtg gcatcgtgaa gagactctgg atgttcgacc
     8161 gtctgaggac ggatgagaaa ctccccggtc tgcacatcga acaacgaagc aagctggatg
     8221 gcgacaggac tccctggtgc cagttccctg atctttgcat agagctgtgc ggccctttgg
     8281 ggattttccg cgtccggatc gtcgcgaaga atctcgatgg ctttccccaa ttcgcccacc
     8341 gccgccatct cgtccccctt ctcccgatag acatgggcaa ggagctcgag aaatggaaca
     8401 cagcgggggc cggcggccaa atactcctgc aacagcgatt cggcgagcca ataatcttgc
     8461 cgctcaatag cttcattggc gagagattcg aatgcttgtc tggccgtgac caagcttcca
     8521 agtctcagat ggagggtcgc aaacaaccgc cgggcctcag tattttgggg atcaagggcg
     8581 aggagttctt tgagggctgc ggacgaccgg ccatatttgc cggtattcat gtgcgagatc
     8641 gcttcttcga gaagggcgct tttcttgctg tagccgacga caccccgctt attaacacgc
     8701 gagggtgctt tcttgtcagg tttctgggag gctctcttgt cggtccgcac gttattaacc
     8761 ttagcagatc attaagaaca caaaccggct tccccagctg gtttcagcct gatatccgct
     8821 ttgaccgcct atggcagact ctgtagatgg ggaaactgga cacagatgtg caatcctgat
     8881 gccatcttga cactctggtt cgaccgcata tttctttaat tttcggtagt tagcgaactg
     8941 tagctaccca accgctgccc ctaattggat tcaaaaatgg acagccatac cgagaatggg
     9001 catcctcata ttggttagga acaagtgcga atcgaccggt cacacccatt gagcatagag
     9061 atttcttccg taaaatcctc aaaatgtaga ggagatgccg acatccatct gccccagcta
     9121 attcgaaatg atcctgcctg ttggttgtgt cgttacacaa ccttatgata cggtcctcca
     9181 ggtcgtttcc tttatttttc acgacaaacg acaatcccct cttgtacaag ctagccgacc
     9241 tgttgtttat gtattttctc aagtcgaatc agcataatcg attacggtgg gctctgattg
     9301 cctgtctgtt agtcctactt ccagcattat tggtacctat tcctctcctc gcagaaagcg
     9361 gcgggggggg gcaaggccgt gtcacgttgg acttcaatga agtcgaactg gccgtctttg
     9421 ttcgctttat cagtgaactg accggcaaaa attttgtttt ggacgatgcg gtgaagaagg
     9481 ccggaggaaa aatcacggtc atctcgccaa cgaaggtttc gccggatcag gcctatagcc
     9541 tcttcgtgtc agcgctggaa gtcagtcggt tggccgtggt tccgaaaggg cccggctacc
     9601 agatcgttcc aatgggagaa ctcccccctg aacgcggagt cttcgtgtac aaattaaagc
     9661 acgccaacgc gacagatctc gcggccgtgc tgaccaactt ggtcgctcga tcacagaccg
     9721 tcgcacagac cgcccccggg gctcgaccgc caatcagacc tttgaccgaa ttcgaagcgc
     9781 ccgtgcaagt gttcgccgac aagccgacga attctatcat catcagcgcg accaaaaacg
     9841 cctatagcaa gttgcaatcc gtgatccggg acctggacac cagacgcaac caagtctttg
     9901 tcgaagcggt catcctggaa gtcacattgg atcggctgag acagatcgga acggatccac
     9961 tccaggttct cgcagccggc gagagcggct cggtgcgggc tatcgggggg ctcaatcggg
    10021 cgccggaaga tttgtccacc atagcccagg ccatcgttgg cgtcgcgacc ggtggaactg
    10081 tgtcggttct gaacaccgtc aacatccgcg ccttcatgca gtttcttctc agcctcaccg
    10141 atacgaatgt actttccaca ccacaggtcc ttgcggcgga taatcagaaa gccaagattg
    10201 tagtcggcga gaatgtcccg ttcccaaccg gacaggcgca gggcatcacg ggaggcactc
    10261 ttgttacgat cgaacgcaaa gacgtggggg ttacattaga attgaccccg caagtgcttg
    10321 aaaatgattt gatccgtctc gagatcaagc aggaaattac cgccatcgag ccaacggccc
    10381 cccaaactat tggtactggc acgtcttcgg taccagtggg tcccaccacc accaagcgct
    10441 ccatggaaac caccacaatt gcaaaagatt tacagacgct tgtcatcgga gggctggtac
    10501 gggacaacat cttgctcact gaaaacaaga ttccctttct cggagacatt cccatattgg
    10561 gttggctttt tcgatcacag agccgtcaga tacagaagct caatctcctc gtctttttga
    10621 cccctcaact cgtccgcgat gaaactgaaa tggtcgaact gaatgcccgc aaagcgaaag
    10681 agctcagcac ttttcagcgc gaaggtcgca tcgaggaacc caccaggctt aagcaagagg
    10741 tcctggaaaa actcgagcgt ccccacacag ctccccctcc ggttcccaac cacgcggaac
    10801 cttccgccgt caccccgcct cccggcaaac ctgactagag gctcacgaaa taccagccgc
    10861 tggcggtacc ctgccacatc tggcaactgc atcgccacac cggaggacca tcgcgcataa
    10921 attgtcagga agaggagcgg catggggtgg atgacgggtt tcgaacccgc gacctccgga
    10981 tccacaatcc ggcgctctaa ccagctgagc tacacccacc acgcagggaa caggtcggga
    11041 ggaaatgaac agggagatgg atattagcaa agagcgaaga aggcccgcaa ctcgcaggat
    11101 ccgtcttaac ttctggaatc aaacgccgct tgcatttcca tgataatagc ggccgccgcg
    11161 gcggccggat cggacgcggc gcgaatcggc cgaccgagca caagatagtc ggctcctgcg
    11221 gcaatcgtct gggtcggcgt cgtggccctt gcatgatcat cgacatcctt gcccgccggg
    11281 cgaacgcccg gtgtcacgat cacgaatcgt ggccccactt tgggccggag cacgcccggt
    11341 tcttcgccag acgctaccac accgtcgcac cccatctcag aggcgaggag ggcgcgcgcc
    11401 gtcaccagct cctgaaccgt atacggaata cccatctctc gcaaatcatg gctatcgaag
    11461 ttggtcagga cggtaaccgc caggagcttg aggggcgagc ttccgcgtcc tttcaccgcc
    11521 gcagccagcg cttttcgatt ggcatggata gtcaggaatt caacacccat cgctgcaacc
    11581 cgttccgtcg cacgccgaac ggtttcctcg atatcgagaa atttgaggtc gagaaagacc
    11641 cgcttccccc gttggagcat tcgctgcaca acctcaggtc ctgccgccgt atacagctcc
    11701 aacccgactt tcacgaaggt aatctggtct tgcacctgat ccatcagccg atccgcctcg
    11761 gccgcggatg gcacgtcgag ggcaagaatc agccggtcac gcgcgatgat cttactcatc
    11821 gatcaatcct tgattcggca gctcaacttg acgatcctgt gaaccatatg atacaaacgc
    11881 gcttctcttg tacaggagtt tgctatgcca gtcatccata aatctacgat tcgtcgagcc
    11941 agacaggccg tgaagcggca ggccaggaac cgtgccacgt tgagcgccgt gaagaacctg
    12001 gtcaagaagg tccaagcagc cgttgccgac aaaaaggtcg acgatgccaa gacctctctg
    12061 cgcgaagcca cgtcggctct cagcaaggcc gtgacgaagg gagtgttgaa acgaaacacg
    12121 gcttctcgcc gcatttcgcg cctcacgcaa cacgtcaatt ccttggccgc gtcgcgctaa
    12181 tcgtccggac attcgacaac gttctcgccc gtgccggccc agcaggcgcc ggagaggatg
    12241 tctcggcttt gcgcgcctca gcttgcgggc ggtcgcacag ttgcaagatc agccggtcga
    12301 ggagcatccc aggcctgccc ccgctgcctc cttttagttt tccatccgct tcaagaaacg
    12361 ctcgcaacgc ctcctggaga tgcgcatcgg gaaactgacc gacaaatgcg cgaactttat
    12421 acggatccat tcgcaacgtc ctggccgcct cgccttctcg ccctcccagt cgcatcaact
    12481 ccttcacttt ccacaaccgg cgatactgcc atgccagcga acccaaaatt ctgagcgggg
    12541 cctcacccgc atccatgttg cgagccacga tggccaaggc ccttcctcgg tccttcgctc
    12601 caatagccgc agccaagtca aatacggacg caccgggctc cgtgccacgc aatgtcgcga
    12661 cgtcgcgagc cgtgacaatc ctgccggccg gtacgtatga agccactttt tcaacttcgc
    12721 gtctgacgga atacagcgag ccgccacagg cttctttcag caagtgaatc gcttcctcgt
    12781 cgatccgcac gccgagacga tcggcctctt gtttgagcca gggcacgagt tgtgagtcct
    12841 tcagcggagc gcaatccacc gcgaccgcgg cctgtatgag agcttgcgta aatttgagtc
    12901 ggccgtcgag cttagaggcc acgaagatga gtgtcgacga ctcgttgggg gccttgaggt
    12961 agggaagcaa cgagtcacat tgcctggcag ggagcttgtc cgccgccttc acaatgacca
    13021 gacggcgcga cgcaaacacg gcgatttcag aagcacagga gacaatctgc gccccttcgg
    13081 cctcatctcc atagaacacg tcacagttga aatcgtcacc gccgtcaccc agcagtgact
    13141 gcttgagttg ggacaacgcg gcatcgcgga gcaggtcttc ttcccctatc agcaaataga
    13201 cgggagctac ctttccctgc gcaagttgtg tcgtcagttg agccactgaa acggcttggg
    13261 gcataaccac gttcagtctt agggtttatc cggttgactt ggcgcggcgt tcgccaccgg
    13321 tttcgtcaac cgaccggatt ccaactgcaa gagaaagcga gacgcaaggt cttctgcgac
    13381 gaatcggcca gcttgttcca aggcccggtt ctggaggacg cggttgaact gcaggtccgt
    13441 cgtcacgtaa aactctgagg cccccttcac cgtttgagtc cagactaccc gcttcgtcct
    13501 cgtctcttca actttgatcg cgaccactac ctccgcccgg ctctctaacg tggtcgtctg
    13561 ggtaaagctg agggtcggca aggtgaccga gagaatgtct ccggacacta tcagatccgc
    13621 ggcttctgcc tgcgggacaa tctgagcacc gcttcccgat gaaaactcat gcctgagata
    13681 attcgtgaac cgcgattcca gattcggctc gaaggtcgag ttcttcaatg tttggatgac
    13741 gacgcgcggc ggcggagtcg aagacgctgc tgccgccggc tctccaatgg tgggaccagc
    13801 accttcgacg cgaaattgat aaccgcaggc gctgagcagg gccagcgaca cacacagtgc
    13861 tgagacgtaa atactgagaa tggtaaggga ccgtaccttc tcgtctcgac tcataccctt
    13921 cttgaattca gctctcagca ctcaggactt cctttagacg acaaagttaa tgagcttctt
    13981 ctcgacgtag accaccttct tgggttcctt tccttgaagc cattcagccg cttcggtacg
    14041 ggccaggctc tccaccacgt cgcgagccgc gtcagcgccg acttcgatct tggcgcgaag
    14101 cctgccgttg acctggatcg gaatcgtcaa tcgatcgctc acagtcaggg ccggatcaaa
    14161 aatcggccaa ggctgctgag agacgctcgc ccgatgtcct aagagggccc acagttcttc
    14221 ggccagatgg ggcgcaaagg gcgaaagcag caacacgaat ggttcgatca aggaccgaga
    14281 ccgctgctcg gtcttcgtca tctcattggt gaagaccatc atttgcgaga tggcagtatt
    14341 gaaccgcagc tgctcgatgt cgtccgtcac tttcttgatc gtctgatgca gcagccgctg
    14401 atgatcctgg ctagggacgg acgatacgat cgcacttgat aaccggcctt cttcgttaac
    14461 catcaagcgc caggcgcgct cgagaaagcg cgtgaggccc tcgacccccc tcgtgctcca
    14521 gggtttcatc gcctccagcg gccccatgaa catttcgtac aggcgcactg cgtcggcacc
    14581 gaactgatcc atcatctcgt cgggattaac gacgttgccg cgtgacttgg acatcttctg
    14641 attgtcttct ccgaggacga tgccctgatg gaccagcttc ttgaacggct ccggcgtact
    14701 caccacccca atatcgtgca ataccttgtg ccagaaccgg gaataaagca gatgcaggac
    14761 ggcatgctcg ctgccgccga cgtagaggtc caccggcatc cagtattgtt ctttaaccgg
    14821 atcaaccagc tgcttggaat tcttcggatc gataaaccgc agatagtacc aacaggaccc
    14881 tgcccattgc ggcatcgtgt tggtttctcg tctggccggt ttgccgcttg cgggatccgc
    14941 ggtcacaagc cagtcttcca gattggcgag cggactctcg ccgcttccgg acggcttaaa
    15001 attgctcgtc tccggaagga ccaacggcaa ctgttcttcc ggcagcggcc gtgcttcccc
    15061 atcgacccat ataataggga acggttcacc ccagtagcgt tggcgggcaa agagccagtc
    15121 gcgcaatttg taattcacgg ccttccggcc gctgccgttt gactccagcc aggccgtgat
    15181 cttcggaatg gcatcgaccg gcgtcaaacc atcgatggaa aaactttggt cgggcgccgt
    15241 ggagttcacc actttgccct tcgccgtctc gacaaaagct tctttgtcga catcaccgcc
    15301 ggcaatgact tcacgaattg gcaaggaata ggtcttcgcg aaggcccagt cgcgtgcatc
    15361 gtgcgccggt actgccatga ttgcgccggt tccgtagccc atcagcacat aatcggcgat
    15421 ccagatcggg agccgttcac cgttgaccgg attgacggca tagccgccgg taaagacgcc
    15481 ggtcttttcc ttttcaagtt cctgccgttg cagatcgctc ttgcgcgcgg ctgcctccct
    15541 gtaggccgtt acggcggctc gacgatcggc aacagtcacg acttcaacca ggggatgctc
    15601 gggtgctaag accatatagg tcgcaccgaa gagcgtatcg ggcctggtcg taaagatccg
    15661 gatccggccg ttgatccccg caattgcgaa ctcgacctcc gcgccgatcg accggccgat
    15721 ccagtttttc tgcatttcga gcgtgctggg cggccactcc accagggaaa gatcgtccag
    15781 gagacgatcc gcataggcgg taatcttcag cacccactgc cgcatgggct tgcggatgac
    15841 gtcgaatccg ccgacctcgc ttttgccatc gacaatctct tcattggcca gcaccgttcc
    15901 caaggccggg caccaattca ccggaacttc tgccacatag gccaggccct tcttgaacag
    15961 ttgcaggaag atccattgcg tccagcgata ataggccggg tccgtcgtgc tgatttcgcg
    16021 atcccagtcg tacgacaatc ccacccgctt catttgccgt ttgaacgtgg cgatgttttg
    16081 ggccgtcgtg atggcaggat gaatacccgt cttgatcgcg tactgttcgg ccggcagtcc
    16141 aaatgcatcc cagcccatcg gatggagcac gttgaacccc cgcatccgtt tgtagcggga
    16201 cacgatgtcc gttgcggtat agccctccaa gtgaccgacg tggagaccgg accctgaggg
    16261 atagggaaac atgtcgaggc aatagaattt cggcttgatg ggatcgtcca tcgccctaaa
    16321 ggggcgatgc tcctcccagt aggcctgcca cttggtttcg atggcgtgat ggtcgtaggt
    16381 cttgctcatg gtatcagggg gcaggttcag aagaagaagg actctaacat agacttctca
    16441 ggctcaacaa gatttcccgg cggcggagaa tctctttgct atactgcgca ctacaatggg
    16501 gctgtacagc cattacatat ttcctctcct gatggactgg gtgatgggcg gagaggagtt
    16561 tcagcggctt cgaactgcgg ttcttcggga tgcgcaggga gacgctctgg aaatcggctt
    16621 cggtaccgga ctcaatctcc cccactatcc cgagaacatt tcgagtctca cgctcgtcga
    16681 cccggccagg ctccttccgc acagggtcac agagcgggcc tcggcggtct cgtttcccat
    16741 ccatacccgg cacatgaccg ccgaatcact cccgtttcac gaccatcggt tcgatacagt
    16801 cgtaagcacg tgggcactct gtacgattcc agatcccgca aaagctttgc gagatgtccg
    16861 ccgagtcctc aagccggacg gacaattcct cttcctggag catggccgga gcgacgacga
    16921 gacggttgcg aagtggcagg atcgactcaa tcctcttcaa aacgtcatcg gttgcggctg
    16981 caatctgaat cgccggatcg accagctgat cgagcagagc gggttgcacc tcgtgcactt
    17041 ggatcgattc cagatgtgca acgttccgcg gatcgctggc gaaatgtatc aagggctggc
    17101 ccgtccataa ggaaggcccg gatcaagaat gcgacagcgg cgtgccgttc aagagatagg
    17161 acagttgctc aatcgacgca aagccggttg tcggagaggg agggagctgc gagctggact
    17221 tggcactgtg gacaatctct gcgataccga atcgacttgc ggcatctaaa caactttcat
    17281 cgtcgtccac atacagcgac cgtttcgggt taaatccgac caggcgctga caggcaggcc
    17341 agtattccgt acgcatcttg aggtagccca cctcgaacgc gtccacaatc cggtcgacct
    17401 gacgatccaa accagtcttg gcggctttca cctccacgcc ggcgcggtga gcgtttgtga
    17461 gaatcgtgat ccgtttccca cgccgctgga gttcacgaag aaatgcttcg gcgcccggta
    17521 gaaaatcaac catgtggtcg agttccttgt gcatagccac gacatcgata cccacccgcc
    17581 tcgtccagta ctggagatcc gtccaagcca gttcgccttc cacagactgg tacatcgcca
    17641 tcagacgatc acgggactct tcaaagagca ggccgtgcat ggccgcatag cgacggggca
    17701 actcttcttc gaaaaagtag ttgtcgaaat gccggtcgag cagcgtcccg tccatgtcga
    17761 ggaggacatc gtcgatctcg ttccagttca gttgaattga agtcatagga gacagtgtac
    17821 ctgaggccgg ttgtgtttgc caaaattcat gtgatacggt ccggtgggag tcaccatgcc
    17881 acgtaccaaa gccgcgccag cgccgacact tccattgccc tccacacaag ggggcccgat
    17941 tcccctcgtc gccattatcg gccggccgaa tgtcggcaag tcgacgctat ttaatcgcat
    18001 actcggtaag aaaaccgcaa tcgtagacga tgtgccggga gtcacacgcg atcggaatta
    18061 cgctgatgcc aactatcgga atcgaccgtt tcgcctcgtc gataccggcg gactcgatcc
    18121 cactgcatcg gaaggaatgt tggcgctgat caagcggcaa tcagaactgg ccatcgccga
    18181 ggccgatatt ctcattcttt tgatggacgg acgaaccgga ttgacgcccg cggaccatga
    18241 agtggtccgc ctgttacgtg ggacgaccaa accgttgttc gtcgccatca acaagatcga
    18301 taccccgaaa gtcgagacat tggtcgcgga tttctatcag ttggggaccg accagcttca
    18361 tccgatctcg gccgaacatg gaatcggtgt ctctgaactg ttagatgaga tctatcccct
    18421 gctccctgtc ctggacgaca agcaagaaca gagccagatg cctcgcatcg cggtggtcgg
    18481 ccggccgaat gtcggcaagt cgacgctggt caacgcggtg cttggcgaag atcgcgtcgt
    18541 cgtgagcgat gtccctggca cgacgcgcga ttccattgat tcgctcgccg tctatcaagg
    18601 gcggcgttat ctgttcaccg atacggccgg cattcgccgc cgcggaaaga tcgaccgggg
    18661 catcgaaggc tacagcgtgg cgcgatcgct ccgtgccatc gggcgctccg atgttgccgt
    18721 cctgttactt gacgcgcagg aaggcgtcac cgaacaggac accaagatcg ccggtgtcgt
    18781 catccgtcaa ggacgcgcct gcatgttaat ggtgaataag tgggatctga gagaaggcga
    18841 tagccaggcg agacagcaag tcgagcagga cctacggcgg cgctttccat ttctctcctg
    18901 ggcgcccgtt ctctttggag ccgcggtgaa acccgattcc cttcgacaac tgttcccgac
    18961 aatcgacgag gtctacgcct ccttctcgaa acgcattccc accggtcagc tcaatcaatt
    19021 tctccaaaat attctggcaa cccatcccct gcccgtccga aaggggaagc cgacaaaaat
    19081 caccaagtca gccttcatga ctcaagtggc tgtgcaaccg ccggtctttg ccctgtttgt
    19141 cggccatccg gacaatatca cgcctgccta cctccgattc ctggaaaacc aacttcgcga
    19201 tgtctacggt ttcaccggca cccctgttcg gctgctcgtc agaaagaaat aacggacagt
    19261 tccgtccgcc aagcagcgac ctttgtacgc gaaccggtct ctgaagccgt tgggggcatc
    19321 gattcctcaa cgattccggc ctccgacctc ataatgggta taggtgcaac tcttgcaaag
    19381 tcttcacggt atttttctgg gaggaatccc gtgccgaaaa cagctcaacg aaaccctgct
    19441 cccgctcctc accacgtctc tcccatgcca agagagacat ccacttggca ggcccctcat
    19501 catagtcata gctccggtcc ttctcctgac acgggattga tggtcctctc ctcctcgatg
    19561 cgtatcctcc atatgaatca tagggctcga tccctcatgg cccagttcgg cgaggcccac
    19621 gagctctggc cccatttgtc cccagaatcc atgccggcga ttctgatcga attttgcggt
    19681 gaagtcctcg ctcagctcca acggcaggac ggaaaccacg aatgggcgga gtttgaaatg
    19741 tgccggatct gtcacatgct gacgcctgcg ctgttgctcc gaggattcgg tgtaccatca
    19801 acggatggac gaacgccccg gatgattctc acactccagc cctgcgttcc ctcatccgac
    19861 cccgcaatgc tccggaaccg gccggatgcg tcaaccagcg catcgcccga tctcggccac
    19921 tcgacacact agcaggatgc cctcgcacag cgtctcgcag tgcctttcaa cttgactcct
    19981 ctagaacatc atggttaatt cgctcggaga cagcaggccg gggccgcagc actgttgtag
    20041 cgcctgatag gaacagcctt ctggaacact ctgcccaaaa tcgcatcaac ccccttcaat
    20101 gatcggcatc gcccacgata ccgtcagcct gcagcgccaa cttcttgccg tcatggtgag
    20161 cgggctcgca accttgctct gtacccatcc cctctcggcc gcagaaccga tcacggcagt
    20221 cacagtcgaa tcccccgctc aagctccagc ggctgagagt gctcccgcta acggatcatc
    20281 cgtcgaagct gatccttcga ttaccttgcg gggaagcgtg tggcgcacca aagccgggat
    20341 tgtgtttctg aaaactcccg tcggcatcct gacgctcagc tccaaaacaa cgttgaagga
    20401 tctgaaaggt tcccatgagg tcgcattctg ggtgcatgac cgcaactcgg tggtcgagat
    20461 ccgcaaacgc agcgacggct cgttagtcca ccggtatttc agcggaccga tgactctggg
    20521 acccgacgcc tcgaagacct tgcgccggtg gtccgcggag ggggaacaga tcgtccattt
    20581 cggcacgcaa gaggaaaagc tcgccgccta ccacgaagga gatccgctca ccgtcgaagt
    20641 cgacgagtcg cagaccgtga tcggagtcca cgatctgcaa ttcgacctcc aggtgagcca
    20701 ggttcctcca gccggctccc aggcccatct gctcctttcg ggcaccgtct ccaagttgaa
    20761 atcctacttc gtgttcttcc ggaccccggt cggcgtggtc atggtcaacg cgaagattgg
    20821 tctgccacgc gtgaaggtcg ggcaggctat gacgttgcat atcgacaatg ggcgggtgac
    20881 cgtcgatctg gcaggcaagt agacagcggc tgttccgttt tgcgacgata tgcctgcttc
    20941 tccgccactt ctccttctga aacggatcga tctcccgttt gacagacccc gctgaactcc
    21001 ctagactcgg gcagacgatg tcttcaattc ctcgacagaa acccagccag ccgcagtccg
    21061 accaagactc cgacgggttt gataaactgt atcgtgatca cgtggacttg atttaccgct
    21121 atgcgcatcg tctctgcgga gaggcagagt cggcgaagga tctggtccag gagacatttc
    21181 tcaatgccta tcgggggctc aagggttttc gcggcgatgc tcaagtttcc acctggctct
    21241 ataccatcgc atcccgggct tgcatgcgca tgcggcggaa acgcaagggg gaaccggagc
    21301 gcgaattgtc gctcgatgac ttcgtgccca cttcggaggg cgagttccgc ctccagattc
    21361 cgatggacgg gctcaccccc gaggaggcgc tccaaaataa ggagctgcgg gtggccctcg
    21421 accaggccat caacaagctg cccaagaaat atcggatgac actggtcctt cgggatatgg
    21481 aaggactgag cgccaaggaa gttggcgcga tcatgggagc gaacgaacgg gcggtgaaat
    21541 cacggttgca ccgtgcgcgt ctgttcgtga gacgtgagtt gagcgcccgt ggaatcacgc
    21601 aggaagacca tcagaccggc aagcaaaggg aatcgggagg gtccaacgca tgacgaagaa
    21661 agcaccggct cgcaagggcc ctgggcaggc ttcgggacgc cgtcatcagc ataggccggg
    21721 caactgcctg aacatcctcc ggcagctgtc ggcctacatc gacgacgaat tgccgtcgga
    21781 tatttgtcgt gaactccgtc gtcacttggg cgcctgtccg aattgcgaag tgttcattac
    21841 atccctccgg cagaccgtca cgctctgtcg ccacagccgg acaccccagc tgtcagcgaa
    21901 ggatcgcatg gcgcttcgcc agaagattct ccgaacagca caatcatgat cggtccaatg
    21961 gctcgactcg tcggccccct tgtcctaatc agcagcctga tcgggattac cgctctctcg
    22021 ttcgccttgc ccccggcgcc gattcaactc gaagagggtc gggcgattta cgaacgtcat
    22081 tgcgcggact gtcacggttc ggaggggcgg ggagatggga aacagtccat ctcgctttct
    22141 ccccgtcccg gcaacttgat ttcggcccag acctcggcga aagccgatca ggatctgctg
    22201 aagatcattg ccaatggacg gcctcgcaca gccatggagg gctggaagga ccggttgtcg
    22261 gaagacgagc agcgcgcggt cctcgcctac attcgatctc tcgtccggtt tacgcggtca
    22321 gcgactcctc ccccgccgac tccctagtcg ttcgaacccg ctcccctcgc ccctttcgga
    22381 tcacgaaatc cgcatggtac agtatcgcct tcatcttgac cttctaagga gtggaaccgt
    22441 catgattcgc ctgttttccc atactcgcag ccggttgatc gtccttgtcc tattcgcatc
    22501 tgcgtcaatc acgctctcga ctgccttcgc agcaccgctt cagaagaagt cgaagggcct
    22561 cgcgcccggc gcctcagttg cgcagcgcta tgcggacgcg gtctcaaaag gcgacaagat
    22621 cgaggtcgcc aaactcgact tcgcatgcca ataccggttg gttgccgcgt caaagtcagg
    22681 cctcgctgct tatcccgatc aggccgacgc ctcctacgac gcctgctggc aaggattcaa
    22741 gaccgctcat gaagcggcac tgaccagagt ggacgtcggc atggatgtgc tttggcccag
    22801 caacggagcc ctggcattct tccgcgaaga gctctatcgc tacccggcct cggcattcgt
    22861 catggatgtt gtcggcctgt cgcctccggg cagcgggttg cacctcaaga cgatcagcac
    22921 gaaggcctta cccaatgcct cgttccacct gcgcccgaat cagccattga tctcggtccc
    22981 agccacactc gtcacgatga cggtcagcta tcaagacccg ctgacctccc ctgtcaccaa
    23041 agcacccggc agttataagt ggacgaatac agtcaagccg gcccgtcgcg cattgaagag
    23101 catcgatctc caatggatcg tgttgtccgg attgaagaag cacgggttcc ctggtgacgc
    23161 agcggtcgtg aatctcttgg tgtcgaccgg cgcaccggtc gatggcatcc cgcaggaaat
    23221 cattcccttt gtgacagaga ccagcaaagc gctgcccggc tcactcgtct ggtgggggcc
    23281 ggaggatgca ccgggtctgt tggtcgccgc cgctgctcgc gc
//
LOCUS       DATIFR010000105        13784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1599748, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000105 DATIFR010000000
VERSION     DATIFR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599748"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLD60_05800"
     CDS             <1..232
                     /locus_tag="VLD60_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44504.1"
                     /translation="MVPPDGQEAPPPLIRIVSGTTKPAEAFAIIRYNDLWYWIDNRDL
                     RSKGTFTFLLILMTLADTGEKAQTPIMTIQAN"
     gene            complement(297..1022)
                     /gene="pgl"
                     /locus_tag="VLD60_05805"
     CDS             complement(297..1022)
                     /gene="pgl"
                     /locus_tag="VLD60_05805"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSB44505.1"
                     /translation="MADILMKINVLADADAVAREAAAVIAAEARASVAARGRFIMAVS
                     GGQTPWIMLRALSTQDVPWDKVHIFQVDERVAPAGHQDRNLTHLRESLLTHAPLSEER
                     IYPMPVEAVDLEAAAEQYTGTLQKIAGSPPVLDLVHLGLGPDGHTASLVPGDPVLEVT
                     ESDVALTGSYQGRRRMTLTYPIINRSRRILWLATGSGKRDMLFRLRDGDRSIPAGLVR
                     SDHALVLVDRAAAAGLGPDSRMK"
     gene            complement(1028..2407)
                     /gene="zwf"
                     /locus_tag="VLD60_05810"
     CDS             complement(1028..2407)
                     /gene="zwf"
                     /locus_tag="VLD60_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSB44506.1"
                     /translation="MNEFQSDALVFYGATGDLAYKKIFPSLQAMVKRGHLNVPVIGVA
                     KAGWTLEQLKARAQASIETHAGGLDRSAFEKLCGLLRYVDGDYQDSATFQAIRRELGS
                     AKRPLHYLAIPPVLFATVIEQLAKSGCHEGAGVVLEKPFGTDLASARELNRILLGTFP
                     ETRVFRIDHYLGKRPIKNMLYFRFANALPETFWNRNHVESVQITMAENFGVEGRGAFY
                     ERTGAIRDVIQNHLFQVLANLAMEPPIRMDSESIRDEKVKVLKAIPPLDEHNLVRGQF
                     RGYRTEKGVAPDSQMETFAALRLEINSWRWQGVPFYIRAGKCLPVTCTEILLRLRRPP
                     SAYPATGLLPNHLRFRVSPDTTLAFGMMAPKTSDEMEGQPFEAMVNRHPQPDEVDAYE
                     RVLTEAMAGDATLFARQDYVEEAWRIVDPVLKQRTPVYEYEPNSWGPGEVERRVMPPD
                     GWQNPVVSL"
     gene            complement(2415..3434)
                     /gene="gnd"
                     /locus_tag="VLD60_05815"
     CDS             complement(2415..3434)
                     /gene="gnd"
                     /locus_tag="VLD60_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSB44507.1"
                     /translation="MQLGMIGLGRMGANMVKRLVKGGHECVVFDMSPKTVQELVREKA
                     VGASSLADLVKNMHKPRAVWLMVPAAVTDKTIADLLPHLEAGDILIDGGNSYYIDDIR
                     RAKELAPKKIHYVDVGTSGGVWGLERGYCMMIGGEKDVVKHLDPIFTTLAPGRGDIPR
                     TKGREKIGGTAEQGYLHCGPVGAGHFVKMVHNGIEYGLMAAYAEGMGLLHQANIGKRE
                     HEIDAETTPLRDPEHYQYEFNLPDIAEVWRRGSVVASWLLDLTATALVEDPKLSQFSG
                     RVSDSGEGRWTIKAAIDEAVPVPVLSAALYQRFSSRGEADYQDKLLSAMRFQFGGHHE
                     KPAKP"
     gene            3737..4849
                     /locus_tag="VLD60_05820"
     CDS             3737..4849
                     /locus_tag="VLD60_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44508.1"
                     /translation="MHQCTTAACRPYANRSNRAMIRRIVTCAAVSIWISLSFNGLVAE
                     AVTLPPVDDLMKELKLSDRDREKVRHGEIVDWSPSEGSDRELALGMVFLVKAKKEDLF
                     EIYRQAMVLREVSVITAHGTLTGQGSMEDLAGVELKPNAEKEAKRYLNAEPGEELNLD
                     AKEMEAFRALQSASKDGQVPVKEVEALIRKGLLARYQGFHTKGLAGIAPYERGSGKQR
                     LAGDELLLSTKQLSGVAKYLPAFHKFLLNYPNGLPEGEAKNLEEYFYWLNIDIFGRPT
                     YVLVQRILYHVGDAAMAVERQYYSSHDYNSMLQGVAVIPTKEGMFLFYIGRVSTDQVA
                     GFTSSALHPVSRAIAAPYIKDMFRDLQARAAKKQKK"
     gene            complement(4904..5689)
                     /locus_tag="VLD60_05825"
     CDS             complement(4904..5689)
                     /locus_tag="VLD60_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44509.1"
                     /translation="MPSQIERGTYAQNREPEAQEQEEANRLSTIAARMGTDPTAIIGR
                     VETLFRYDALEGGKRTNNLVARLDVPYHGNFVFRADLPYVWSSPNQPGTSNQKGVSDL
                     FVRAGGRVYTDPANVLFAGMDFTFPTADQQLGSGKYTVGPGVATAHVLPAWGSLFYTL
                     LQHQASVGGDPSRQAVSLSRIQGWFNTIWTDQWWTRLEVVTQVDWERKATNSMTLEFE
                     GGYRFVKDWGVWARPGVGLWGRNVAGAYDWNIEVGIRRMFTTF"
     gene            complement(5874..7316)
                     /locus_tag="VLD60_05830"
     CDS             complement(5874..7316)
                     /locus_tag="VLD60_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB44510.1"
                     /translation="MEPGWIDSGAAEKPQPIIRAGGDPPSDESQSASAAMPAGGPPNL
                     FKGLTFWRVLIILSVVIAVTAFFVEWQRGLVLEYFRQGRAFDRFALVLLAIVILPRQF
                     ERFGLPGMLGLILGGVLVGQWGLALAREVGAVGHFFSEMGRVFLMFMAGLEISLSDFR
                     RYAKPSTLFGVATFGGPMIGGFLLGQVFGYGINASVLIGSLLASHTLLGYPILEKLGL
                     VRRPFALTTIGATIFTDIASLLVLAVCLSVHESGILSWYMLAALIGSLAVYCVFVMGA
                     IPWFGRFYLRLRHDDEIAQFQFILAVIVICAVGAKLIGLEDIVGAFLCGIAVNQVLQH
                     GPAREKVEFLGRSLFIPGFLFVIGAELDPPAFVRNLIQGLPFVLGMIAVLCLGKFAAA
                     SITAWIYRYSQPEGLSMWALSLPQLAATLAATVTAYKSINAAGERLIGEEVVNAIIVL
                     MVLTATVGPILTEWFGRSLQESPAPEPAKG"
     gene            complement(7344..8126)
                     /locus_tag="VLD60_05835"
     CDS             complement(7344..8126)
                     /locus_tag="VLD60_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44511.1"
                     /translation="MGINYNFVAFLLVLGLFLGMLAMLALGQRLGKRTFKQESDLIHT
                     RLTGVEAAIFGLIGLMIAFTFSGAAARYELRRQLVVEEANAIGTAYLRLDLLPAASQP
                     ALRDLFRRYSTARIAVYRVLPDIEASNAQAANATALQKEIWTGVLAALKEAPAQATIV
                     VTPALNQMIDITTTRAIAARTHTPILIFSVLLILGLVCSFLAGYVLANARTRNVRLHM
                     VAFAVVITLTIYVIFDLDYPRFGLIQLDFADQALIDLLAGMK"
     gene            complement(8290..9267)
                     /locus_tag="VLD60_05840"
     CDS             complement(8290..9267)
                     /locus_tag="VLD60_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44512.1"
                     /translation="MRLLIALGIFWIVGSGTGSLSIAAENSAGSTDEPASAEPEKPQE
                     APGGLRGRIESRFYGATPEEHARLEEERKRLSAAAAGFGTDPTAINGYYQLEYSHSTF
                     TNNLRSDSALAEARLPITPNFVMRVTMPYVFADLNQPRGFTTNGASDLTVRNGGRLYA
                     TPDVALFVGGDIVFPTGSNDRLSTGKYTIGPTGAVAVPLPRLRSLFFTFVQDFNSVGG
                     DPSRANIHFARVASAFNTIWSERWWTLASMEWDMDWNRNRKTTMNLLGEVGHRFDKHW
                     NVLISTGAGVMGRDTFLGLDWTVQAGVRWVFQTPFFSEQVVESLPMPGK"
     gene            complement(9735..9998)
                     /locus_tag="VLD60_05845"
     CDS             complement(9735..9998)
                     /locus_tag="VLD60_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44513.1"
                     /translation="MTDAKQQLIKATEHLKQQRDELRVKLHLAKAEAKDEWARLEKQW
                     EEMRPKLEAAKEEAGKTAESVGTALGLAVEELKKGYERLRSRL"
     gene            complement(10133..10843)
                     /locus_tag="VLD60_05850"
     CDS             complement(10133..10843)
                     /locus_tag="VLD60_05850"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HSB44514.1"
                     /translation="MQSFTRITVCVLALVVAAGCASTETSKRKILVTEKIPRPGHIWL
                     YDFAATPAEIPTESALAGKPVEHATPQTPEQVAAGRQMGALITTTLVEEINGMGLLAA
                     RASSGTKPQINDLVIRGYLLSIDEGDATKRVAIGFGSGASQLSVAVEGFQVTAQGLRK
                     LGSGNVDAGGGKTPGGAVGVAALIVTGNPVGLIVGGGAKAYGEYSGSSKIEGRAKAIA
                     KEIAEKLRPRFQQQGWIQ"
     gene            complement(11182..11661)
                     /locus_tag="VLD60_05855"
     CDS             complement(11182..11661)
                     /locus_tag="VLD60_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44515.1"
                     /translation="MMVLQIGVLLLSFLFMGQAQAQEAAQMNMEILKEKIKADKKLVV
                     AANMNLTDAEAKNFWPLYDGYQKELEQINQRLLTTIKSYADAFNAGKGELSNDTAKKL
                     LGEVLAVEESEVKLRQSYAAKMGKVLPATKVARYLQIENKIRAIVKFELAAQIPLVS"
     gene            complement(11737..12297)
                     /locus_tag="VLD60_05860"
     CDS             complement(11737..12297)
                     /locus_tag="VLD60_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HSB44516.1"
                     /translation="MRCQTLTAVVLSMILGLSMQASMAIAGQSAEAAQIDREADAALK
                     KLLEDTPEAEMFRKDAKGILVFPSIVKGGFILGAHYGKGTLKKGGGTAGYYSTVAASY
                     GLQAGVQSFGYILFFMNEKSLEYLDNSEGWEVGVGPSIVVMDKGAAKSLTTTTGRSDV
                     YAFIFSQQGLMAGLGLQGSKITRIDP"
     gene            complement(12432..12854)
                     /locus_tag="VLD60_05865"
     CDS             complement(12432..12854)
                     /locus_tag="VLD60_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44517.1"
                     /translation="MTRPRSLTVIAWLALAQGMIGGLAGLLWLQIVSIFDQGNGALSS
                     IMVMIAEAKGWILITLALMYVLFAAGAWKVRSWAWAVGLLVSVLTLLYLFAVLLRGGS
                     VVAVVLWLIVPVIMISYLLSSEGRQAFGQDGGLSRPLH"
     gene            complement(12864..>13784)
                     /locus_tag="VLD60_05870"
     CDS             complement(12864..>13784)
                     /locus_tag="VLD60_05870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSB44518.1"
                     /translation="QETEADEVGQKIAAAAGWDPLELATFLGNLEREVTLHEKEPRKP
                     SFFDSHPATPDRVADTTRHAKSLTRAKRDPISPTREAYLARLNGVVVGPRAANGIFRG
                     SAFLHPDLNIFVQFPEKWPHENAPGKIVAAAPKGDAAVVLEGVATGDDPLEGARVLGK
                     AIQVDLAAKAESLTINGLPAARAVVPTDGRAHAEVTWIAHGGLVFQFLTIAPTEQSKT
                     FQPLFESVTHSFRQLSPAERAGITEKRIRLITARKGETIEAVAARAHSAWKVDEVAVS
                     NGLNVTAPLREGQVLKVAVEERYESQEPRR"
BASE COUNT         2890 a         4292 c         3750 g         2852 t
ORIGIN      
        1 gatggtcccg cccgacgggc aggaggctcc cccgcccctg attcggattg tgagcggaac
       61 gaccaagccg gctgaagcct tcgcgattat tcgatacaac gacttgtggt attggatcga
      121 taaccgtgat cttcgctcca aaggcacgtt caccttcctc ctcatcctga tgaccttggc
      181 cgacaccgga gagaaggccc agacgccgat catgacgatc caggccaatt gaaccatgat
      241 cacccacaga atcacgttca gaaactttct acgaatcacg ccctgccggc tctcttctac
      301 ttcatgcgtg agtccgggcc taatcccgct gccgcggcac gatcgacaag caccagggcg
      361 tgatcactcc gcaccagacc ggcgggaatc gatcggtccc cgtcgcgcaa gcggaacagc
      421 atgtcacgct ttccgctccc ggtcgccagc cagaggatac ggcgtgaacg attgatgatc
      481 ggataggtca acgtcatgcg acgccgtcct tggtagcttc cggtcagcgc gacatcgctc
      541 tcggtcactt caagcacagg atctcccggc accagcgacg ctgtatggcc atcaggccca
      601 agcccgaggt ggaccaaatc aagcaccggc ggcgagccgg cgatcttctg gagcgtcccg
      661 gtgtattgtt cggccgctgc ctccaggtcg actgcttcca ccggcatggg ataaatgcgc
      721 tcctctgaga gcggcgcatg tgtgagcaag ctctcgcgca gatgtgtcaa gttacggtcc
      781 tggtgacctg cgggggccac acgttcgtcc acttgaaaga tgtggacctt gtcccaggga
      841 acgtcctgcg tcgacaacgc gcgcaacatt atccagggcg tctggccgcc gctcacggcc
      901 atgataaatc tgccgcgcgc ggctacggag gctcgagcct ccgccgcgat gacagcagct
      961 gcctctcgtg ccactgcgtc ggcatcggcc agcacattaa tcttcataag gatgtcagcc
     1021 atgcggccta gagtgagacg acgggatttt gccagccgtc cggaggcatc actcttcgct
     1081 caacctcacc cggcccccag ctgttcggct cgtactcata gaccggcgtc cgctgtttca
     1141 gcaccggatc cacgattcgc cacgcctctt ccacataatc ctgacgtgcg aacaacgtcg
     1201 cgtcgccggc catggcttcc gtcaacaccc gctcgtacgc atccacttca tcaggctggg
     1261 gatgacgatt gaccatcgcc tcgaacggtt gcccctccat ctcatcactg gtttttggag
     1321 ccatcatgcc gaaggcgagt gtcgtgtcgg ggctgacccg gaatcgcaga tggttcggca
     1381 acaaacctgt agccggatag gccgagggcg gccgacgcag tctcaagagg atttccgtgc
     1441 atgtgaccgg caggcacttg ccggcccgga tatagaatgg caccccttgc caacgccagg
     1501 aattgatttc caggcgcagg gccgcgaagg tttccatctg cgaatccggg gccactccct
     1561 tctccgtcct gtacccgcgg aactgaccgc gcacgagatt gtgctcgtcc agcggcggga
     1621 tcgccttcaa gactttcacc ttttcatccc gaatggattc gctgtccatc ctaatcggag
     1681 gctccatggc cagattggcc aggacttgga acaggtgatt ctggatcaca tcgcgaatgg
     1741 cccctgttct ctcatagaac gccccccgac cctcgactcc gaagttttcc gccatcgtga
     1801 tctgaacact ctccacatga ttgcggttcc aaaaagtctc tgggagcgca ttcgcaaagc
     1861 ggaagtacaa catgttcttg atcggccgtt tcccgagata atgatcgatg cgaaagaccc
     1921 ttgtttccgg aaacgttccg aggagaatgc ggttgagctc ccgcgccgag gcaaggtcgg
     1981 tgccgaacgg cttctcaagg acgacgcccg ccccttcatg gcaaccggac ttcgcgagct
     2041 gctcgatcac ggtcgcgaac aacacaggag gaatcgccag atagtgcagc ggtcgtttcg
     2101 cagagccgag ttccctgcgg atcgcctgaa acgttgcaga atcctggtaa tccccgtcca
     2161 cgtatcgcag taaaccacac aatttctcga atgcggaacg gtcaaggccg ccggcatgcg
     2221 tctcgatgct ggcttgggcc cgagccttga gctgctccag ggtccagccg gccttggcca
     2281 ccccgatgac cggaacgttg aggtggccgc gcttgaccat cgcctgcagc gacggaaaga
     2341 tcttcttgta ggcgaggtcg cccgtggccc cgtaaaaaac gagggcgtcc gattggaact
     2401 cgttcattcg gccctcatgg cttcgccggt ttttcgtggt ggccaccgaa ctggaatcgc
     2461 atcgccgaca atagcttgtc ctggtagtcc gcttcgccgc gtgagctgaa ccgctggtac
     2521 agcgcggccg agagcaccgg caccggcacg gcctcgtcga tggccgcctt aatcgtccac
     2581 cgcccttcgc ctgaatcaga cacccggccg gaaaattgtg aaagcttggg atcctctacc
     2641 agagcagtcg ccgtcaggtc cagcaaccac gaggcgacta cgctgccgcg ccgccagact
     2701 tcggcgatgt cgggcaggtt gaactcgtac tgataatgct cgggatcgcg cagcggcgtc
     2761 gtttcagcat caatctcatg ttcacgcttg ccgatgttgg cctgatggag caggcccatg
     2821 ccctcggcat acgcggccat gaggccatac tcgatcccgt tatgcaccat cttgacgaaa
     2881 tgcccggcgc cgacaggacc gcagtgcagg tagccctgct cagcagtgcc gccgatcttc
     2941 tcgcgtccct tggtcctggg aatgtcgcct ctgccgggtg ccagcgtcgt aaagatggga
     3001 tcgaggtgtt tcaccacgtc cttctcgcct ccgatcatca tgcagtaccc gcgttcgagt
     3061 ccccatacgc cgccgctcgt gccgacgtcc acatagtgaa tctttttcgg agcgagttct
     3121 ttggcgcgcc ggatatcatc gatgtagtac gagttgcccc cgtcgatgag aatgtcgccc
     3181 gcttcaagat gcggcagcaa gtcggcgatg gtcttgtccg tgaccgccgc cggaaccatc
     3241 aaccagaccg ccctcggctt gtgcatgttc ttcacgaggt cggcaagcga cgaagccccg
     3301 acggcctttt cacgaaccag ttcctggact gttttcggag acatgtcgaa gactacgcac
     3361 tcatggcctc ccttcaccaa ccgcttcacc atgttcgcgc ccattctgcc aagaccgatc
     3421 attccgagct gcatgatatg tccctttcct taaaattgat tgttcacggg ttggacgtca
     3481 atgcttcgtg gtcaacaaca cctcgaaacg gcttcagaac tgcgaccgcc tcatcacgaa
     3541 acacgaatga ccgatgcgtg acggcaccgg aaccggattt tgcgctgtag ttggcccacc
     3601 tatcaagaaa tttgtggagg gagggatagg aatgttctcc agagagccgc ctccatgcct
     3661 tgactttcaa agggcaatat aggaaatatt gggcctgctt ctcaccgctt catacagaaa
     3721 gatctggaga caattcatgc atcaatgcac aacagcagcc tgtcgccctt atgccaaccg
     3781 gtccaaccga gcgatgatac gccggatcgt cacctgcgcc gccgtcagta tctggatcag
     3841 cctgtcattc aacggactcg tcgctgaggc agtgaccctc ccgccagtcg atgatctcat
     3901 gaaggagttg aagctctcgg accgcgatcg ggagaaagtt cggcatggag agatcgtcga
     3961 ttggtcaccg agtgaagggt cggatcgtga gctggcgctc ggcatggtct ttctcgtgaa
     4021 agccaagaaa gaagatctct ttgagatata tcgacaggcg atggtgctga gagaggtatc
     4081 ggtgatcacc gcccatggca ccctcacggg gcaaggcagc atggaggatc tggccggggt
     4141 ggaattgaag ccgaatgcgg aaaaggaagc caagcgttat ctgaatgcag aaccgggcga
     4201 ggaactcaat ctggatgcaa aagagatgga ggcctttcgg gcgcttcagt cagccagcaa
     4261 ggatggacaa gtgcccgtga aagaggtgga ggcgcttata cgaaaggggt tattggcccg
     4321 gtaccagggc ttccatacga aagggctggc aggcattgcg ccctacgagc gtggaagcgg
     4381 aaagcaacga cttgcagggg atgagttgct cctgtccacg aagcaattga gcggggtggc
     4441 caaatacctc ccggccttcc acaagttttt gcttaattat ccaaacggct tgccagaagg
     4501 agaagcgaaa aatctggagg aatacttcta ctggctgaat atcgatatct tcgggcgtcc
     4561 gacttacgtc ctggttcagc gcattctcta tcacgtcggc gacgcggcca tggctgtcga
     4621 gcgccagtat tattcgagcc acgattataa cagcatgctc cagggcgtcg ccgtcattcc
     4681 gaccaaggag ggcatgtttt tgttttacat cggccgggta tcgacggatc aagttgcggg
     4741 gttcacgtcg tccgccctcc atcccgtgtc tcgcgcgatc gcggcaccct atatcaaaga
     4801 catgttcaga gatcttcaag ccagagcggc aaaaaagcaa aaaaagtagc cgcgtgagtc
     4861 cagcacctac ttcgtcaatc ttgcgggggg accggggcac cgcttaaaac gtggtaaaca
     4921 tgcgccggat gccgacttcg atattccaat cataggcgcc agcgacattc cgtccccaga
     4981 ggccgacccc gggtctggcc cagactcccc aatcctttac gaatcgatat cccccctcaa
     5041 attccagcgt catgctgttc gttgccttcc tctcccaatc cacttgagtc acgacctcga
     5101 gcctggtcca ccattggtcc gtccaaatcg tgttgaacca gccttgtatg cgagacaggg
     5161 agacggcctg tcgagaagga tccccgccca ccgaggcttg atgctgaagc agcgtgtaga
     5221 aaagggaccc ccacgcgggt aatacgtggg ccgtcgccac acctggtccg accgtgtatt
     5281 taccactccc caactgctga tccgctgtcg gaaacgtgaa gtccatcccg gcaaacaaca
     5341 cattcgcggg atccgtatat acacgcccgc cggctcgcac gaacagatcg ctcaccccct
     5401 tctgattgct tgtaccgggc tggttcgggc ttgaccagac gtatggcaag tccgcacgaa
     5461 agacgaaatt tccgtgatac ggaacatcga gccgggctac cagattgttg gtccgtttgc
     5521 ccccctccaa ggcatcatat cgaaagagag tctcgactcg cccgatgatc gccgtgggat
     5581 cagtacccat cctggcagca atcgtagaca gacggttcgc ttcttcttgc tcttgcgcct
     5641 ctggttcgcg attctgagca taggtgcctc gttctatctg agatggcact atttcctgtc
     5701 cgaacgacag gtggtgagga ctaagcagaa agagaaacca ggcgcatcct gccagaatgt
     5761 gagtatgaaa tcgcgtcctt gctctaaccg ccggacgatc gttcacaact cgccgccgaa
     5821 tataatccgg ttcaccatcg cggagaggtt gcaaggagac cgtgataaaa atattacccc
     5881 ttggctggtt caggtgccgg actctcttgg aggctccgcc cgaaccattc ggtcagaatt
     5941 ggcccgacgg tcgcggtcag gaccatcagc acgatgatgg cattgaccac ttcctccccg
     6001 atcaatcgct cgccggctgc gttgatcgat ttataagccg tcaccgttgc agcaagcgtg
     6061 gcggcgagtt gtgggagaga cagcgcccac atcgaaagtc cctcaggttg actgtaccga
     6121 tagatccagg ctgtgatgga tgccgcggca aatttgccga gacacaagac cgcgatcatg
     6181 ccgagcacga acggcagtcc ctgtattaag tttctcacga aggccggcgg atccagctcc
     6241 gctccgatga cgaagagaaa tccaggtatg aagagactcc ttcccaggaa ctccaccttt
     6301 tcccgcgccg gtccgtgctg gagcacctga ttgacggcga tgccgcagag aaaggccccg
     6361 acgatgtcct ccaggccgat caatttcgct cctacggcac aaatgacaat aactgcgagg
     6421 ataaactgga actgggcgat ttcatcatcg tgacgcagcc ggagatagaa cctgccgaac
     6481 cagggaatcg cacccatcac gaacacgcaa tagaccgcca gcgatccaat caatgcggcc
     6541 agcatatacc aggacaaaat cccgctctcg tgaacggaca gacacactgc aagaaccagc
     6601 agcgaggcga tatcggtaaa gatggtcgcg ccgatcgtcg tgagggcgaa gggcctgcgc
     6661 accagaccca gcttctcgag aatcggatag cctagcaatg tatgagatgc cagcagcgag
     6721 ccgatgagca cagaggcgtt aataccgtag ccgaacacct ggccgagcag aaatcctccg
     6781 atcatgggtc cgccaaaggt ggccacaccg aatagggtgg agggtttggc ataccggcga
     6841 aaatcgctca aactgatttc gagtcctgcc ataaacatga gaaacacccg ccccatctcg
     6901 ctgaagaagt ggccaaccgc tcctacctct ctagccaggg cgagacccca ttgcccaacc
     6961 aacaccccgc caagaatcaa tccgagcata cccggcaacc cgaatcgttc aaactgccgg
     7021 ggtaagatga cgatggccag caggacgagt gcgaaccggt cgaaggcccg accctggcga
     7081 aagtattcaa gaaccaaccc ccgctgccac tcaacgaaga acgccgtgac ggcaataacg
     7141 actgatagga tgattaacac tcgccagaag gtcagaccct tgaacagatt cgggggccca
     7201 ccggccggca tcgcagcgga agccgattgg ctctcatcgg aaggaggatc accgccggca
     7261 cgaatgatgg gctgaggttt ttctgcagct ccactgtcga tccatcctgg ttccattttt
     7321 atgcctcctg agaatccagc ggcttatttc atcccggcga ggagatcgat gagcgcctgg
     7381 tcggcgaaat cgagttgtat caagccgaat cgcggatagt cgagatcgaa gatcacatag
     7441 atcgtgagtg tgataacgac cgcaaaggcg accatgtgga gcctcacatt gcgcgtcctg
     7501 gcattggcca acacgtaccc ggccaggaaa gaacagacca gcccgagaat cagcagcacg
     7561 gagaaaatta atatcggggt gtgcgtgcgc gccgcaatgg cgcgagtggt cgtgatgtcg
     7621 atcatctggt tcagcgccgg ggtcaccaca atcgtggcct gcgccggggc ctctttcagc
     7681 gcagcgagca ctccggtcca gatctccttc tgcaacgccg tagcattcgc cgcctgggca
     7741 ttcgacgcct cgatgtcggg caagacccga tagacggcga tccgcgctgt ggaatagcgg
     7801 cggaacaggt cccgcagcgc cggttgagat gcagcgggta gcagatccag cctcagatac
     7861 gcggtgccga tcgcgttcgc ttcctccacg accaactgcc ggcgcagctc ataccgggcc
     7921 gccgcgcccg agaaggtgaa cgcgatcatc aagccgatga ggccgaaaat agccgcctcg
     7981 acaccggtca gacgcgtgtg aataagatcc gactcctgtt tgaaggttct cttccccaag
     8041 cgctgcccga gtgccagcat cgccaacatc ccgaggaaca aacccaagac gagcaagaaa
     8101 gcgacgaaat tataattgat gcccatttct cctccgatgc ttgccctact cctcaaatcg
     8161 aacggcacga aggccaacga gcaaacaaca acgagtgctt cgatcagagc atatataatc
     8221 gaagaggcac gcgagtctcg ctcgtgcgag tgtcgtctct gttcaacccg gtcacggtct
     8281 aacctcgtgt tactttcctg gcatgggaag cgattcaacg acttgttcgg aaaagaacgg
     8341 ggtctggaac acccaacgca cgccggcttg tactgtccag tccaaaccca aaaatgtatc
     8401 ccttcccatc actcccgcgc cggttgagat aaggacgttc caatgtttat caaaccggtg
     8461 gccgacctcg ccgagcaggt tcatcgtggt ctttcggttt ctgttccaat ccatgtccca
     8521 ttccatggat gctagggtcc accatcgctc ggaccagatg gtgttgaacg ctgacgcgac
     8581 tcgcgcaaaa tggatgttgg cgcggctggg atcacctccg acagagttga agtcctggac
     8641 aaatgtaaag aacaacgagc gcagcctggg taacggaacc gccaccgcgc ctgttggccc
     8701 aatggtatat tttcctgtgc tcagtcgatc gttcgagcct gtcggaaaca cgatatcgcc
     8761 gccgacgaac agggccacat ccggggtggc atacagccgt ccgccgtttc gaaccgtaag
     8821 atcactcgcg ccattcgtcg tgaatccgcg gggctggttg agatcagcga acacataggg
     8881 catggtcacc cgcatgacaa agttgggtgt gatgggcagt ctggcctctg ccaacgccga
     8941 atcgctgcgc agattgttcg tgaaggtact gtgagaatat tcgagttggt agtagccgtt
     9001 gatggcggtg gggtcggtcc cgaatcctgc tgccgccgcc gacaggcgct ttcgttcttc
     9061 ctcgaggcgc gcgtgctcct caggtgtggc gccgtagaac cgcgactcta tcctgccacg
     9121 taggccaccc ggagcctctt gcggtttttc cggttctgca ctggccggct cgtcggtcga
     9181 tcccgcagag ttttccgccg caattgagag cgagcctgtt ccggaaccga caatccaaaa
     9241 gataccgagc gcaatgagga gcctcattct cacgtcccgc ccattcggga acagtgcgaa
     9301 tcccaggtcg cggatccctt tcccgcatga ccagacagca gcggaaattg tcccgggccg
     9361 gcgagactgt gcgccagaat ttatccctcc cattgggcgt attcggtcac atgcgacgca
     9421 gcgaacatgt tcaccatcgg ctcgaccttg cgccttctcc tcactcgaca atccccgacc
     9481 ccgggttccc actctcaacg gtcagccttg tatcagatcc gagcgactcc ctacaaggcg
     9541 agcagcgacg gacgttcagg acagcgggag agggtcagca gcgagattgg gctctacctt
     9601 cgagacgaca cagggcgctc ccgcgaagcg aaagcgccct gttctaattc accgagaatt
     9661 actcactcca tcagacctta cgcgcacccg cgacaagatg gctccagctg ataatcaccg
     9721 tcgggatgag cccttcacaa ccggctccgc agccgctcat agcccttctt caattcctcg
     9781 acggccaggc ccagcgccgt gccgacagat tcagccgtct tgccggcttc ctccttcgcc
     9841 gcctccagtt ttggcctcat ctcttcccac tgtttctcca gcctggccca ttcgtctttc
     9901 gcctccgcct tcgccaggtg cagcttgact cgaagctcgt cgcgctgctg tttgagatgc
     9961 tccgttgctt ttatgagctg ctgctttgcg tccgtcatgt tgcttcctcc ttgtttgact
    10021 caactacctc atgccagcag attcatgcac catcaggttg catgcgtgcg gctcttcttc
    10081 atacgagcgc tcgcttcacg tcaaatcagc tacctatcct tcgccaggtc tgttactgaa
    10141 tccagccctg ctgctggaac ctgggcctga gtttttccgc gatttccttg gcaatcgcct
    10201 tggctcgacc ttcaatcttg ctgctgccgc tgtattcccc atacgctttc gccccgccgc
    10261 cgacgatgag gccaaccggg tttcccgtga caatcaaggc ggcaactccc accgcacccc
    10321 ctggcgtttt gcctccgccc gcatcgacgt tgccggagcc gagttttcgg agtccctgag
    10381 ccgtcacctg gaaaccctcg accgctacgc tcaactggga cgccccggag ccgaatccga
    10441 ttgccacgcg ttttgtcgcg tcgccttcgt cgatcgaaag gagatagccc cggatcacaa
    10501 ggtcgttgat ctgcggtttg gttccactcg aagcccgcgc cgccagcagc cccatgccgt
    10561 tgatctcctc gaccagcgtc gtggtaatga gagctcccat ctgccggccg gctgcgacct
    10621 gctcgggggt ttgaggcgtg gcatgctcga caggcttgcc ggcaagcgcg gattcggttg
    10681 gaatctcggc gggcgtggca gcaaagtcgt atagccagat gtgaccgggc cgaggaatct
    10741 tttcggtcac gagtatcttg cgcttggagg tttcggtcga agcacacccg gcagccacga
    10801 ctaaggccaa cacacatact gtgatacgcg taaaggattg catggcagac ctcctattat
    10861 gatgaggcgg attggtcagc tgaacctatt gtgggtgggg aatcgttaac gggccgggct
    10921 ctttgggtat tccctgcgac cggttgcgcg gtgctggagg ttgcaccgcg cgtcgctctc
    10981 gccgacgaga ggcccgtgtc gaatgtcaga cctgcctgcg tcgaggccgc gccgacggct
    11041 gcgtatgcgt tgacgcatac caccaaccgc cgagcgctgc gcccgccacg acgacgaacc
    11101 cgagcgggtg tcccgctgcc caccggtcca ttgcgattta ccagccccgt actcggtact
    11161 tcgccgtcgt tctgaagtcg attaactcac gagcgggatc tgtgccgcca gttcgaactt
    11221 cacaatggcc cgaatcttgt tttcgatctg cagatagcgg gcgacttttg tggcagggag
    11281 caccttgccc atcttggccg cgtacgattg ccgcaacttc acttccgact cttcaacggc
    11341 cagcacttcc cctaagagct tcttggccgt gtcgttggaa agctcgcctt tgcccgcatt
    11401 gaaggcatcg gcataactct tgatcgtggt cagcaatcgt tgattgatct gctccagttc
    11461 cttttgatag ccatcataca agggccagaa gttcttggcc tccgcatcgg tgagattcat
    11521 gttggcggcg acaacgagct tcttgtccgc cttgattttc tctttcaaaa tttccatgtt
    11581 catttgtgcc gcttcctgag cctgagcctg acccatgaac aggaacgaca gcaacaacac
    11641 cccaatttgc agaaccatca tgacgcactc tcccttctgt gttgactcac cattgcatac
    11701 tatccacccg taccggcgcc ctcatggcac cttgatttac gggtcgatcc ttgtaatctt
    11761 cgacccttgc aatcccaagc cggccatgag tccctgctgg ctgaagataa aggcataaac
    11821 gtcgctccgg cccgtcgtcg tcgtgagact tttcgccgca cctttatcca tgacgacgat
    11881 gctgggaccg acaccgacct cccatccctc gctattgtcc aggtactcca gtgacttctc
    11941 gttcatgaaa aacaggatat agccgaacga ttgtacaccg gcttgaagac cgtacgacgc
    12001 ggccacggtg ctgtagtaac cggccgttcc tccgcctttc ttcagcgtgc cttttccata
    12061 atgcgcgcca agtatgaacc cgcccttcac gatgctcggg aacacgagga tgcctttggc
    12121 gtccttccga aacatctccg cttccggggt gtcctcgagc agtttcttca gtgccgcgtc
    12181 ggcctcgcgg tcgatctgcg ccgcctcagc cgactgccct gcaatcgcca tcgaagcctg
    12241 catcgagagt cccaggatca tggacagcac gacggccgtc agcgtctgac acctcatgtt
    12301 acacctcatt gatgtattcc tccgctcgcg caagatcaaa agcataccga ctttcaaaca
    12361 attcttctac tgcgattttc gtagggggag ctccgtgtgt cattgcggcg cacggcgggc
    12421 ctgtcagatt ctcaatgcag cggacgggag agcccaccat cctgaccaaa cgcctgtctc
    12481 ccctccgaag aaagcaagta cgagatcatg atgaccggaa cgatcagcca gagtacgacc
    12541 gccaccacgg atcctcctct gagcaggacc gcgaacaggt acagcagagt cagcaccgag
    12601 accaacaacc cgaccgccca ggcccagctt cggaccttcc acgcccccgc tgcaaatagc
    12661 acatacatca gcgcgagcgt gatcaagatc catcccttgg cctcagctat catcaccatg
    12721 atcgaagaca atgccccgtt tccctggtca aagatgctga cgatttgcag ccacagcaat
    12781 cctgccagcc ctccgatcat cccttgcgcg agggcgagcc aggctatcac tgtcaggcta
    12841 cgtggccgtg tcatctcgtt tccctatcgt cgcggctctt gcgattcata ccgttcttca
    12901 actgccactt tgagcacctg cccctcacgg agcggcgccg tcacgttgag tccattggag
    12961 acggctactt catcgacctt ccacgccgag tgggcgcgag cggccacggc ctcgatcgtc
    13021 tcgcccttcc gggctgtgat taatctgatt cgcttctccg tgatgccggc gcgctcggct
    13081 ggagacagct ggcgaaaact gtgcgtgacc gactcgaaca gcggctgaaa cgttttggat
    13141 tgctctgtcg gtgcgatggt caggaactgg aacaccagcc ccccatgggc gatccatgtg
    13201 acctcggcat gggcacgacc gtcggtgggc accacagctc gcgcggccgg aagaccgttg
    13261 atggtcaggg actcggcttt tgcggccaga tcaacttgta tggccttgcc gagcacccgt
    13321 gccccttcaa gcgggtcgtc acctgtcgcg acgccctcga gcacgaccgc tgcatccccc
    13381 ttcggcgcgg ccgccacaat tttccccggc gcattctcgt gaggccactt ttccggaaac
    13441 tgcacgaaga tattcaagtc cggatgcagg aacgccgatc cgcgaaagat cccgttggct
    13501 gcgcgcggcc cgacgaccac gccattcaac cgggccagat aggcttcgcg tgtgggactg
    13561 atcggatcgc gcttggcacg ggtcaacgat tttgcatggc gcgtggtatc ggcaacccgg
    13621 tcaggagttg ccggatgcga gtcgaagaaa ctcggtttcc gcggttcttt ttcatggagc
    13681 gtcacctcac gttccaggtt gccgagaaat gtggcgagtt caagagggtc ccagccggct
    13741 gctgcggcaa ttttctgccc gacctcgtct gcctctgttt cctg
//
LOCUS       DATIFR010000106        20593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2174595, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000106 DATIFR010000000
VERSION     DATIFR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174595"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            819..908
                     /gene="kdpF"
                     /locus_tag="VLD60_05875"
     CDS             819..908
                     /gene="kdpF"
                     /locus_tag="VLD60_05875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HSB44519.1"
                     /translation="MNVMYVLGGILSLGLLVYLMVALLKPEWF"
     gene            908..2713
                     /gene="kdpA"
                     /locus_tag="VLD60_05880"
     CDS             908..2713
                     /gene="kdpA"
                     /locus_tag="VLD60_05880"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HSB44520.1"
                     /translation="MTINGLVQIGLYFVLLLALAKPLGWYMARVYEGQACGLDRVLGP
                     LERLIYRLCGVRQTEEMDWKTYGIAMLLFNAMGLVALYALQRLQGFLPLNPAGLGAVT
                     PDLAFNTAASFATNTNWQAYGGETTLSYLTQMLGLTVQNFVSAATGMAILVVLIRGLA
                     RRSAMTIGNFWTDLVRSTLYILLPLALLLSLLLVSQGVVQTFASYQTVTLLQPASYAK
                     PVMDANGQPVLDEQGQPKTEPATAVEQVLAVGPAASQIAIKQLGTNGGGFFNVNSSHP
                     FENPTPWTNFLEVLAILLLPAALCYTFGMMVGDTRQGWALLAAMTILLVCFIPIGLWA
                     EQSGNPAFTALGIDQTSGAGQSGGNMEGKETRFGIANSVLWASATTAASNGSVNSMHD
                     SYTPLGGLVPLFLMQFGEVVFGGVGSGLYGMIVFAIIAVFVAGLMVGRTPEYLGKKIE
                     PYEMKMASLLILIMPIIVLGFTAIGVATEAGTSSILNPGAHGFGEILYAYSSQGNNNG
                     SAFAGLNANTPFYNLTGGIAMLISRFWLAIPTLALAGALARKKLVPAGPGTLPTYTPL
                     FVVLLIGVVVLVGALTFVPALALGPIVEHILMVSR"
     gene            2739..4823
                     /gene="kdpB"
                     /locus_tag="VLD60_05885"
     CDS             2739..4823
                     /gene="kdpB"
                     /locus_tag="VLD60_05885"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HSB44521.1"
                     /translation="MTTQGKRRQLFDPQIMRQAMLESFRKLDPRHQIRNPVMFVVTVG
                     SVLTTVLFFQALFGTGEAPVWFILAVSLWLWFTVLFANFAEAMAEGRGKAQAESLRSA
                     RRELNAKKLGKPESGDQYLSVPSSIVPASALKRGDVVLVEAGDFIPCDGEVIQGVASV
                     NESAITGESAPVIRESGDRSAVTGGTRVLSDWLVIRITASPGETFLDRMIAMVEGAKR
                     QKTPNEIALNILLAALTVIFLLATATLLPFSLYSVQSVSQGAPVTVTVLVALLVCLIP
                     TTIGGLLSAIGIAGMDRMVQANVIAMSGKAVEAAGDVDVLLLDKTGTITLGNRQATAF
                     LPADGVSNQALADAAQLSSLADETPEGRSIVVLAKEQYGLRARDIGELGATFIPFTAQ
                     TRMSGVNLTGREIRKGSADAIEAYVRVHEGQFSVAVRSSVEAISTQGGTPLVVAEGPN
                     VLGVIALKDIVKGGIKERFGELRRMGIKTVMITGDNPQTAAAVAAEAGVDDFLAQATP
                     EAKLKLIRDMQAGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNLVDLDSN
                     PTKLIEIVEIGKQLLMTRGALTTFSIANDVAKYFAIIPAAFASTYPALGVLNIMGLAT
                     PASAVLSAVIFNALIIVFLIPLALRGIRYQPMGAGLLLRRHLLVYGFGGILVPFVGIK
                     LIDLLLVALHLI"
     gene            4834..5466
                     /gene="kdpC"
                     /locus_tag="VLD60_05890"
     CDS             4834..5466
                     /gene="kdpC"
                     /locus_tag="VLD60_05890"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HSB44522.1"
                     /translation="MWTQLRPALVSLVTLTLITGLLYPMVITVLAQILFPWQANGSLI
                     LVDGKPVGSSLIGQPFDAPKHFWGRPSATSPFPYNAAASSGSNLGPTNVTLMKSIQAR
                     IDALRAVDPGNPLPIPVDLVTASGSGLDPHISPAAAEYQVRRVARVRGLDETVVRRLV
                     AQHTEGRQLGVLGEPRVNVLTLNLALDAHGQENRSHDHSRNQRSTVFGLS"
     gene            5567..8272
                     /locus_tag="VLD60_05895"
     CDS             5567..8272
                     /locus_tag="VLD60_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HSB44523.1"
                     /translation="MEERRPDPDALLKRVQAEEAKQARGKLKVFFGATAGVGKTYAML
                     GAAHEQQNDNVDVIVGWVETHGRAETEALLEGLTILPPRVVEHRGTTLREFDLDAALA
                     RRPQLILMDELAHTNAPGSRHPKRWQDVKELLKAGINVYTTVNVQHLECLNDVVTQIT
                     GVRVSETVPDSVLEQADDVELIDLPPDDLLQRLKDGKVYMPEQAQHAIQNFFRKGNLI
                     ALRELALRRTAERVDQQMEVYRRDHAVVRTWPAGETLMVCVNMKPRGPRLVRAARTMA
                     TGLHAKWIAVYVQTPRHLRMPQADRDRVNQTLRLAELLGAETVVLSGANVAQELLSYA
                     RTRNVTKIIVGKPVRARWKEWVFGSVVAELVQQSGETDIYVITGEAGESRPLATQVVK
                     RSSDWSSYGLGVVGVGLSTGVAWLMFPYFGLANLIMVYLMGVVLVATRYGRGPSVLAS
                     VLSVAAFDFFFVPPYLSFGVSDIEYLLTFAVMLIVALTISGLAVRTKQQAELARHQER
                     RTAVLYALSRDLATHRGLATLTQLAVKHVREVFDSQAAIFVADINKHLVLQRGEQLFF
                     EFDPKESGVAQWVFEHGERAGPGSDALPGASALYLPLVGSRGPIGVLAVRPTLATRFL
                     DLEQLHLLEVLANQIALALERARLAEEAQQAHVSVETERMRNAILSSVSHDLRTPLAT
                     ITGAASGLLEEKNQLGSTARLELAKSIYNEAHRLDRLVRNLLDMTRLESGAVQLQKEW
                     HPFEEIIGAALTRLESRLNGHQVRTNFQQDLPLVLLDGVLMEQVLVNLLENAIKYAPP
                     STAIELSAKVSDHELVFEIADRGPGIPPGDERRIFDKFYRAGPAREGGVGLGLTICRG
                     IIEAHGGRIWVERRRGGGAVFRFTLPIEQPQPMIEPEKAERHPAV"
     gene            8298..8996
                     /locus_tag="VLD60_05900"
     CDS             8298..8996
                     /locus_tag="VLD60_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44524.1"
                     /translation="MVTHEATVLLIEDEAEIRRFLRTSLPAHGYRLYEATTATDGLAQ
                     AKARNPDVILLDLGLPDVDGTEVIRQVREWSAIPIVVLSAREQEQAKVAALDLGADDY
                     VTKPFGVNELLARMRAALRHIAGSPGEPTEPVFTLGDLKVDLGRRQVFVSGKETHLTP
                     IEYKLLTTLIRYAGKVMTHRQLLKEVWGPLHVEEGHYLRVYMRQLRNKLEKNPAHPRY
                     LVTELGVGYRLRTE"
     gene            9213..9605
                     /locus_tag="VLD60_05905"
     CDS             9213..9605
                     /locus_tag="VLD60_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44525.1"
                     /translation="MFHRTYGLVMASLIAGAIGGFAASVIMGTPGVAQQSDQTTFQVV
                     RTRDFQLIDAEGRTRGRIGFSAETQPYLQLVDEKDIAGVWVGVARETGVAVRDTDGKT
                     RLVLSVDETGNPSLVVRNRQHQMRSFQP"
     gene            complement(9743..10381)
                     /locus_tag="VLD60_05910"
     CDS             complement(9743..10381)
                     /locus_tag="VLD60_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44526.1"
                     /translation="MRQFAAFAMLLSLTILWGCSNTVLVTVPPRMDLRNYETLGIVEF
                     TSNSDPAINSYATQQFQEHVQGAYPGTPILELGSREAVLEAVGATRFDAEAMTKIGKK
                     YGVSAVFLGDIMYSEPKTDVRLTDIARLEGGLRTEVRGDVSGKLMETRSGASVWNSSA
                     WARRQIGRVSVSAKEGVSAAMRDSNPRRDMVPALIFHLTEDFRPRSVRQQKE"
     gene            10919..11368
                     /locus_tag="VLD60_05915"
     CDS             10919..11368
                     /locus_tag="VLD60_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44527.1"
                     /translation="MVDNWAEVKADDKTVAGILAAFNRAEAALEAGNVPDLMPLYSDQ
                     YSYHGLNKTDLQKVWEDLVSHHREFRSNHILSRIVTVPGDPLSAEVTCTGSLWAISKE
                     TGQQVNIDSWYGEVHHMHFENGAWRVRGHRGEAPKTPAFGAAPHPFF"
     gene            complement(11543..12589)
                     /locus_tag="VLD60_05920"
     CDS             complement(11543..12589)
                     /locus_tag="VLD60_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HSB44528.1"
                     /translation="MKHTRIIVTHYGGPDALQVLEEECPEPKDGEVRVRVLAAGVSLP
                     DLMMREGIHPETPALPFTPGWDLIGVVDRLGDGISGLESGQIVAALPITGAYAEFVCL
                     PQRDLVPVPSGLDAAEAVSLVLNYVTAYQMLHRSAKARRGQRVLIHGAAGGVGSALLQ
                     LGHLAGLEMYGTCSSRGASTVSDLGGVPIDYQRQDFVKEIHRLTSDGVDVVFESIGGT
                     HIWRSRKALRSGGTVVAYGITSSLRGGGLASGHSGGRHRFRAIAIFGLYIAGGWLLPA
                     RKRVVPYSIQWLKRLRPALFRQDLIALFDLLQQQKIKPLIAQRLPLSEARHAHELLGT
                     GGVTGKIVLVPNSA"
     gene            complement(12720..13352)
                     /locus_tag="VLD60_05925"
     CDS             complement(12720..13352)
                     /locus_tag="VLD60_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44529.1"
                     /translation="MRSLLHRAGLAVPVLWLLSGALLVQGCYSKAPPPGVRFPITSGS
                     HTVLPTAQQRILLWGDPPLADVALEWLRSHHYSSILMPEKGPFHTPQVSYSFSDRKAA
                     LAVAKEMKADFVLFLEREESKEGALIEPQCGSLFHVSVNVRGLSVESGDTALRGSAHY
                     PHCVDPSGETSRNLTCQAFATAWGFRPSGQLDIPSSLMCTSGQTEPTPIR"
     gene            complement(13841..14155)
                     /locus_tag="VLD60_05930"
     CDS             complement(13841..14155)
                     /locus_tag="VLD60_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44530.1"
                     /translation="MSVKLYTNMVEAIQELKKRGFTANFEFLNQSFRDVDSGRTFKAD
                     ELTIVEHYRFEGASDPDDMSVVYAIEVDGGPKGIIADAYGLYANPNLGGFLEKVNIRE
                     EK"
     gene            complement(14370..14843)
                     /locus_tag="VLD60_05935"
     CDS             complement(14370..14843)
                     /locus_tag="VLD60_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44531.1"
                     /translation="MRKRIISHVQKETASPDQDWLNVEGLAEVEITSEDAAHPIEAAL
                     LPGQASGWRAAGPGKQTIRLLFHHPQRLRRIWINFLEPGTERTQEYVLRWSPDSGQSF
                     REIVRQQWNFSPQGTTCETEDYHVELPAVTVLELNINPDISRGNAFASLAQLRLA"
     gene            15209..16456
                     /locus_tag="VLD60_05940"
     CDS             15209..16456
                     /locus_tag="VLD60_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44532.1"
                     /translation="MPRLPTRPFERPMPDLDELEDSGLSSDHPALQDHIKLGTAWLCV
                     TVTGIGALVYSTISQFSRTSTMLGLIGLLIAYAAYGYSLRKKNTLQFADSLYYMGFLW
                     ALFALIETFLVWPAPKLTADAVLTTFGYALVTTFSGMLLRLLVVQFQVTVPDRLVHAQ
                     ETIDRRVAALTQQLNEATMEITSFRDRAASDLGGTLQDLVRSLEDVRETITEQQRTMT
                     TMMSAGFESSLKDILGRLAAIQIPQEILTTEVAKLIAALGKRGEDVEEAVQKLVKSLM
                     QATDTVTRFGDSLYGSDAAKRIGVAVNDLSSTIKDRTEEFVKMTTALETSRTELDSQL
                     SSLQSLRSAFAKVSTQLSTLETELRDLSPSALRADVRSGLLDVQKSIQSSLDASRAIE
                     STMRGVMFFLKERVTEEHAFDGK"
     gene            16446..17069
                     /locus_tag="VLD60_05945"
     CDS             16446..17069
                     /locus_tag="VLD60_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44533.1"
                     /translation="MESESRSGWALGLSLVEVMLLLVFAALMVSVTDNVESRGQEPPQ
                     SVDKPKNPDAILQTRLDAAMEKSEDLERQLNEVTRFVNELKLMVGARVPTKEGFHEAI
                     ESLKRGYALCQKDNNTLIEASVQNGVETLQVIGEIPSDLVVNFEKGDQTSDLDQIVLF
                     IQDVFQYETDHKCRFNYRLKYATDNDYRKAREGFEKYFYPEKMIRAG"
     gene            complement(17139..17468)
                     /locus_tag="VLD60_05950"
     CDS             complement(17139..17468)
                     /locus_tag="VLD60_05950"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HSB44534.1"
                     /translation="MALGNQPQRGQTYREQALKIFPWICAHCGREFDGKKLSQLTVHH
                     KDHNHDNNPPDGSNWELLCLYCHDNEHQRYLASDASGEVPPSREPDRPFTPFAHLANV
                     LKRKHAD"
     gene            17669..18103
                     /locus_tag="VLD60_05955"
     CDS             17669..18103
                     /locus_tag="VLD60_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44535.1"
                     /translation="MERINRFTLEKHDGPYETWPLHSRLFLDGKPTDLSLPGYDLLHQ
                     FETSDWYILITDYDCPYEEITNFALISKHLSLISCRWLGWMYETFLLERIERIDERTF
                     IAVIGGSDHCWRLSIRSWGIPYIRPRLKLQWLANARSDCRLD"
     gene            18075..18866
                     /locus_tag="VLD60_05960"
     CDS             18075..18866
                     /locus_tag="VLD60_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HSB44536.1"
                     /translation="MLVVIADSIEQGHQTAASLVGQAIKKNPTLRLGLAAGNTPIGFY
                     QSLVDLHQSTHLDFSGVEFFSLDEFLGLPPGSSMSYEAFFHQHLFRSVNADPAHIHLL
                     KAEPHGDIFSYCHSYERLINDRGGIDVQLLGIGINGHLAFNEPMSSFRSRTRMCLLTP
                     ETRENLSRTFNETEVPEWAVTMGLGTIGEAQTLLMLAFGKEKAKAIARAVEGPLTAAI
                     PASSIQLHPNAVVILDPAAAGDLKYKNYYRQQSSRLAMLLPRGLA"
     gene            19135..20172
                     /locus_tag="VLD60_05965"
     CDS             19135..20172
                     /locus_tag="VLD60_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44537.1"
                     /translation="MMVIRAGWSKRMWALAGIGILLLLSVDPSWPTAEATRPIQIHKR
                     GEQTFAFPGSSERDAAPRVNRPYFDRAGQLRWADALVHEQATGSCDTHPLQENPNGYQ
                     IYPRRTVLLSPRVDVRLCSRKGASSETTVIGLTDDGHKVWERETVFRSGVHRLDQYLI
                     GSSREGLVLSSLEVWSPDTGQTIVPASTRSITPESREVPLYAYTGAALYHPARHTVFV
                     YDADVTLTKKRGGLVEINPSTGATTLVHPVVTTLFRGFDRIEEMALMSGGRYLCLAQR
                     LSWRGPTDVSFTILDLATKRLLFHERFCQDSHDTCADPHVVIGPSGAIGFSYSNLNRR
                     EHHLLLYQGRE"
     gene            20174..>20593
                     /locus_tag="VLD60_05970"
     CDS             20174..>20593
                     /locus_tag="VLD60_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44538.1"
                     /translation="MKMFVAWAGTIVGLCLASFGWAGPSSDRDAIHKTLLQSRHLGAH
                     GLGYNAESYAELARRLSPAAIPTLLDLLVHDKEVRVGVVVGLASQCGAAIEPILNAAE
                     RGEVPFISLSDLHDALAQMERAAICSSMDQEQAVKAIM"
BASE COUNT         4419 a         5680 c         5981 g         4513 t
ORIGIN      
        1 ctgtgaacca gtcgaaccga ctactgcaat gattttcttg tcagtcacgt ctatctcctt
       61 ggtaagtagt tacccattag ttctttgcga cgattgccgc gccttcggca agcgctctca
      121 gcctggagta ctcctagtcc cactctgcgc gtgtcgtgaa gagggcgcag agcgctttca
      181 gtgttggaac cgcggagact ggagaatcga tcgcgttagg aaggaccagg aagcgcgagg
      241 gatgagacag tcggcgtggt tgaaacgcct gggatcgatg ctacgagcga aagggccact
      301 tgtcaagcag ccttatccgt gtgctctcgt gtcgtcgaac cacacacgag acagaccgaa
      361 gaaaccagat ggaccaaatt cctgccacgc gccagaaatg atcggactaa caaggactcc
      421 cgaacccgtt tctgatcctg tgaggtcgac tggtccgaag aaggagcaca ccacgtacag
      481 ctagcttacg cgtcttacgc agccttcgaa gtcacgagtt tactccgggt ataaggtcgc
      541 gatttgcgcg ggcggcaggt cgttactgcc agcccggcac cgttgtattc aatcagaaac
      601 aattgttcca aaagctccgc ggtttttacg ccttttttac ttcctgtact ttactatgaa
      661 tgcgcacaag tagagaaaga cagatggaca acattttctt tgccgtcgat tgaggggtat
      721 gcacatggat ctcatctttc tgaccatcgc agtgatattt tttctgctgt ccggttggct
      781 catctcggca ttggatcggc tgtaaggggg ggcacgccat gaatgtgatg tacgtactcg
      841 ggggaattct gtcattagga ttgttggtct atctcatggt cgcgttgctg aaaccggagt
      901 ggttctgatg acgatcaatg gactggttca aatcggactc tactttgtct tgctgctcgc
      961 gctggccaag ccgctgggat ggtatatggc acgggtgtac gaaggacaag cctgcgggct
     1021 cgaccgggtg ttgggccctc ttgagcggct tatctatcgc ctgtgcggag tgcgtcaaac
     1081 cgaggagatg gactggaaga catacgggat cgccatgctg ctattcaatg cgatggggct
     1141 ggtcgcgctg tatgcattgc agcgactcca aggattcctg ccactgaacc cagcggggtt
     1201 gggagccgtc acgcccgatc ttgctttcaa cacagccgcg agcttcgcga caaataccaa
     1261 ttggcaggcg tatggagggg aaacgacact gagctatctg acgcagatgt tggggctcac
     1321 cgtgcaaaat tttgtgtccg cggcaaccgg tatggcgatc ctggtcgtgt taatcagagg
     1381 cctggcgcga cggagcgcca tgaccatcgg caatttttgg accgatctcg tccgcagcac
     1441 actctacatt ctcctgccgc tggccctgct cttgtccctg ctgttggtgt cccaaggcgt
     1501 cgtccagacc ttcgcgtcat accagaccgt cacactgctc cagccggcca gttacgccaa
     1561 gcccgtaatg gatgcgaacg gccagccggt gctggatgaa cagggccagc cgaaaacaga
     1621 gcccgcgacg gcagtcgaac aggtacttgc ggtcggaccg gcagcctctc agattgcgat
     1681 caagcagctc gggaccaacg gcgggggttt cttcaacgtc aattcctcgc atccgttcga
     1741 gaacccaacg ccatggacca atttcttaga ggtattggcg attctgctcc tcccggcggc
     1801 gctctgctac acgtttggga tgatggtcgg agatacgcgc cagggttggg cgctccttgc
     1861 tgcgatgacg attctcctgg tttgtttcat ccccattggg ctgtgggcgg aacaaagcgg
     1921 caatccggcc ttcaccgctc tagggatcga tcagacttcc ggggcaggac aatcaggcgg
     1981 gaacatggaa ggcaaagaga cgcgattcgg catcgctaat tccgtcctgt gggcctctgc
     2041 aacgaccgcg gcctcaaatg gctcggtgaa ttcgatgcac gattcgtata ctcctttggg
     2101 cggactggtc ccgctcttcc tgatgcagtt cggagaagtg gtgttcggcg gagtcggctc
     2161 cggactctac ggcatgatcg tcttcgccat cattgccgtg ttcgtcgcag gactgatggt
     2221 gggacgcacg cccgaatacc tgggcaagaa gatcgagccc tatgagatga agatggcctc
     2281 gttgctgatc ctgatcatgc ccatcatcgt gttggggttt acagcgatcg gcgtggccac
     2341 cgaggccggt acttcgtcga tactcaatcc gggagcgcat ggcttcggcg aaatactgta
     2401 cgcgtactct tctcaaggca acaacaacgg cagcgcattc gcagggctca acgcgaatac
     2461 tcccttctac aacctcaccg gcgggatcgc gatgctgatc tcccgcttct ggttggcgat
     2521 tcccacgttg gccttggccg gagcgctggc gaggaaaaaa ctcgtaccgg ctgggcctgg
     2581 aaccctgccg acctacacgc cgctgtttgt cgtactactg atcggcgtcg tcgtgctcgt
     2641 gggagcgctc accttcgtgc ccgcccttgc cctgggacca atcgtagaac atatcctgat
     2701 ggtgagccga tgacggtact ggcgtggagg atttcgagat gacaacgcaa ggaaagagaa
     2761 ggcaattgtt cgatcctcag attatgcgac aggcgatgct ggaatcattc cggaagcttg
     2821 atccgcgcca ccaaattcgc aatccggtca tgttcgtggt cacggtcggg agtgtactga
     2881 cgaccgtcct gttcttccag gcgctgttcg ggacaggcga agcgcctgtc tggttcatcc
     2941 ttgccgtatc tctctggctc tggttcaccg tgctgttcgc gaattttgcc gaagcgatgg
     3001 cggaaggccg cggaaaggca caggcagaat cgctgcggag tgcccggcgt gaattgaacg
     3061 cgaaaaagct gggtaagccg gagtccggcg accagtatct tagtgttccc tcctccatcg
     3121 tcccggcgag cgccttgaag cggggagacg tcgtgctggt ggaggccggt gattttattc
     3181 cctgcgacgg ggaggtcatc caaggagtcg cttcggtgaa cgaaagtgcg atcaccggtg
     3241 aaagtgcccc cgtcattcga gagagcggcg atcgcagcgc cgtgacgggg ggcacacgcg
     3301 tcctgtcgga ttggctggtc atacggatca cggccagccc cggcgagaca tttctggacc
     3361 ggatgattgc gatggtcgag ggggccaagc gccaaaagac gccgaatgag atcgccctca
     3421 acattctgct ggcggcactc acggtcatct ttctcttggc gacggccacg ttgctcccct
     3481 tttcgctcta cagtgtgcag tcggtcagtc agggggcccc tgtcacggtg acggtcttag
     3541 ttgcgctgct ggtgtgtctg atcccgacga cgatcggagg attgctgtct gccatcggaa
     3601 ttgccggcat ggatcgtatg gtgcaagcca atgtcatcgc catgtcaggg aaggcggtcg
     3661 aggcggcggg cgatgtggat gtgctgctgc tcgacaagac gggtaccatc acgctgggca
     3721 atcgtcaggc gaccgccttc cttccggctg acggggtgag caaccaggcg ctggccgatg
     3781 cggctcagct ctcgtcgctg gcggacgaga cgcctgaggg gcgcagcatt gtcgttctgg
     3841 ccaaggaaca gtatggattg cgcgcgcgcg acattggcga gttaggtgcg acatttattc
     3901 cctttacggc tcaaacccgc atgagcggcg tcaatttgac tggccgggaa atcaggaagg
     3961 gctcagccga tgcgatcgag gcctatgtca gagtgcacga gggacagttt tctgttgctg
     4021 tgcggtcgag cgtagaggcc atctccaccc agggcgggac gccgctggtt gtcgccgagg
     4081 ggccaaacgt gctgggtgtc atcgcgctca aagacattgt gaaaggcggg atcaaagaac
     4141 gattcggaga gctgcgacgc atgggcatca agaccgtcat gatcaccggc gacaatccgc
     4201 agactgcggc ggccgtggct gcggaagctg gtgtggacga tttcctggcg caggccacac
     4261 cggaagcgaa gctcaagctg attcgcgaca tgcaggccgg cggacggctc gtcgcgatga
     4321 cgggggatgg cacgaacgac gctccggcgc tggctcaggc tgacgtcgcc gtggcgatga
     4381 acaccggcac gcaagccgcc aaagaagccg gcaatctggt tgatctggat tcgaacccaa
     4441 caaagctcat tgagatcgta gaaatcggca agcagctctt gatgacccgc ggtgccctca
     4501 ccacattcag cattgccaat gatgtcgcga agtatttcgc gatcattccc gcggccttcg
     4561 cctcgaccta tcccgcgctc ggcgtactca atattatggg attggctacg ccggccagtg
     4621 ccgtgttatc ggccgtcata ttcaacgcgc tgatcatcgt gtttctcatt cccctcgctc
     4681 tccgtggtat ccggtatcaa ccgatgggcg ccgggctgct gctccgccga catctcctgg
     4741 tctacgggtt cggagggatt cttgttccat tcgtgggtat caagctcatc gatttattgc
     4801 ttgtcgccct gcacctaatc tgaggaggca tacatgtgga cccaacttcg cccggctctg
     4861 gtgtctcttg tcaccctgac cctgatcacc gggcttctgt acccgatggt gatcacagtt
     4921 cttgctcaga ttctgtttcc gtggcaagcc aacgggagcc tgatccttgt cgacggcaag
     4981 ccggtcgggt cttcgctgat cggccagccg ttcgacgcac cgaagcattt ttggggccgt
     5041 ccttccgcta cgtcaccgtt cccgtacaac gcagccgcct cgtccgggtc gaatctgggt
     5101 ccgacgaatg tcacgctgat gaagtccata caggctcgta tcgacgcgct cagagctgtg
     5161 gatcccggca accctcttcc tataccagtt gatttggtca ccgcttccgg cagcggatta
     5221 gacccgcaca tcagtcctgc tgcggccgaa tatcaggtcc gtcgcgtcgc gcgagtgcgg
     5281 ggactggatg aaacggtcgt tcggcggctc gtggcacaac atacggaggg acgtcagctc
     5341 ggagtcctgg gcgaaccacg ggtcaatgtg ctgacgttga atcttgccct ggacgctcac
     5401 gggcaagaga accgctcgca cgaccattcc cggaaccaac gatcaacggt gttcgggttg
     5461 tcgtgagaca gcaggagctc tcgttgaccg attgtggcat ctcttctaga ctgaggcgga
     5521 catttcaatc cgccctgatt tccgtgttac cgatcgcaca catctgatgg aagaacgacg
     5581 acccgatccg gatgccctgt tgaagcgcgt gcaggctgaa gaggctaagc aggcgcgcgg
     5641 caagctcaag gtctttttcg gcgccacggc cggtgtggga aaaacgtatg ccatgctcgg
     5701 agcggcgcac gagcagcaaa atgacaatgt ggatgtcatc gtcggatggg tcgaaacgca
     5761 cggtcgtgcg gaaacggaag ccctgctgga aggactgacg attctgccgc cacgcgtcgt
     5821 ggagcatcgc ggaacgacat tgcgggaatt cgatctcgat gcagctctcg cccgtcgtcc
     5881 tcagctcatt ctcatggacg aactggccca caccaatgcc cccgggtcgc ggcatccgaa
     5941 acgatggcag gacgtgaagg aactcctgaa ggctggcatc aacgtttata cgaccgtcaa
     6001 cgtgcagcac ctggagtgcc tgaacgatgt ggtgactcag atcaccggag tgcgggtgag
     6061 tgagacggta ccggactcgg tgctcgaaca ggccgatgat gtcgagctga tcgatcttcc
     6121 ccccgacgac cttctccaac gtctgaaaga cggcaaagtc tacatgccgg agcaagcgca
     6181 acatgccatc cagaatttct tccgcaaagg gaatctgatc gcgttgcgcg aattggctct
     6241 ccggcgtaca gccgagcggg tcgaccagca gatggaggtg taccgccgcg accatgcagt
     6301 ggtccgtacg tggccggccg gcgaaaccct catggtctgt gtgaacatga agcccagggg
     6361 accgcgttta gtgagggctg ccagaaccat ggcgacgggt ctccatgcga aatggatcgc
     6421 cgtgtatgtt cagactccca ggcatctgcg catgcctcaa gccgatcgtg atcgagtgaa
     6481 tcagacactt cgtctcgccg agctgcttgg cgccgagacg gtggtgctga gcggagccaa
     6541 tgtcgcgcag gaactgttgt cctatgcccg cacgagaaat gtgacgaaga tcatcgtggg
     6601 aaagccggtc cgtgccagat ggaaggaatg ggtgtttggt tcggtcgtgg ctgaactcgt
     6661 gcaacagagc ggggagacgg acatttatgt catcacgggg gaggccggtg aaagccgtcc
     6721 gttggcgacg caagttgtga agcggagcag tgattggtcg tcctacgggc tgggtgttgt
     6781 gggagttggc ctgtcaacgg gcgtggcgtg gctcatgttc ccgtatttcg gcctggcgaa
     6841 tctcatcatg gtgtacttga tgggagtcgt actcgtggcg acgcggtatg ggcgggggcc
     6901 gtcggtcctc gcatcagtcc tgagtgtcgc cgcattcgac tttttctttg tccccccata
     6961 tttgtccttc ggcgtgtcag acattgaata tctcctcacc ttcgccgtca tgttgatcgt
     7021 cgcgctgacg atcagcgggt tggcagtccg taccaagcag caggctgaac tcgctcggca
     7081 tcaggaacgg cgtacggcag tgctctatgc cttgagccgt gatctggcca cacaccgtgg
     7141 gctggccacc ctcacgcaac tggccgtgaa acacgttcga gaggtattcg atagccaggc
     7201 ggccattttt gtggcggata tcaacaaaca tctggtcctt caacggggcg agcaactgtt
     7261 cttcgaattc gatccaaaag aatcaggcgt ggcgcaatgg gtcttcgagc atggggagcg
     7321 ggcgggcccg ggcagcgatg ccttgcccgg cgccagtgcg ttgtatcttc cattagtcgg
     7381 atcaagaggt cctatcggcg tgctggcggt tcgtccaacg ctcgcgacgc gtttcctgga
     7441 tctggagcaa ctccatctcc tggaagtgct cgcaaatcag atcgccctcg cgctggagag
     7501 agcgcgcctg gcagaagagg cgcagcaggc gcacgtctcg gtggagacag aacggatgcg
     7561 caatgccatc ctcagttcgg tttcccacga tctccggacc cccctggcga cgattaccgg
     7621 agccgccagc ggtctccttg aagagaaaaa ccagcttggt tccactgcgc gtcttgagtt
     7681 ggcgaagtct atctataatg aggcgcatcg gctggatcga ctagttcgaa atttattaga
     7741 catgacgagg ctagaatcag gcgcagtcca gttgcaaaaa gaatggcacc cgttcgaaga
     7801 gatcataggg gcggcattga cgcgtttaga aagtcggttg aacggacatc aagtgcgcac
     7861 taatttccag caagacctgc cgctcgtgct cttggatggt gtcttgatgg aacaagttct
     7921 cgtcaacctg cttgagaacg ccataaagta tgcgccgccg tccacggcca tcgagctgtc
     7981 tgcaaaggtt tctgatcacg agttggtttt tgagatcgct gatcgagggc ccgggattcc
     8041 acccggcgat gaacggcgca tcttcgataa gttctatcgg gccggtccgg cccgtgaagg
     8101 gggagtgggg cttggcctca cgatttgccg aggcatcatc gaagctcatg gggggcgcat
     8161 ctgggttgaa agacgtcgcg gtggcggcgc cgtgttccgg ttcactcttc ctatcgagca
     8221 acctcagccg atgatcgagc cggaaaaggc cgaacgccac ccagccgtgt gaggggatgg
     8281 aaggatcctg aactgtcatg gtaactcacg aggcgaccgt tctcctcatc gaagatgaag
     8341 cagagatccg gcgatttctt cggacctcct tgccggccca cggctaccgg ctctatgagg
     8401 cgaccactgc cacggatggg cttgcgcagg cgaaggcgag aaatcctgac gtcatcctgc
     8461 tcgatctggg tcttcccgat gtggatggga cggaggttat tcggcaagtc cgcgagtggt
     8521 ccgccattcc gatagtcgtg ctgtcggcgc gtgagcaaga acaggcaaag gttgccgcac
     8581 tcgatttggg cgcggacgat tatgtaacca agccgtttgg agtcaacgag ctgctcgcgc
     8641 ggatgcgtgc cgcacttcgc cacatcgccg gatccccggg agaaccgacc gagccggtgt
     8701 ttaccctcgg cgatctgaag gtggatctcg ggcggcgaca ggtgtttgtc tcggggaagg
     8761 aaactcacct cacgccgatt gaatacaagc tgctcaccac gctgatccgg tacgccggca
     8821 aggtgatgac tcaccgtcag cttctgaagg aagtgtgggg tccgctgcac gtggaagaag
     8881 ggcattatct gcgagtgtac atgcggcagt tgagaaacaa actggagaaa aacccggccc
     8941 atccgcgcta tctggtgacg gagttgggag tgggataccg gcttcggaca gaatgaccca
     9001 ccgcaaccca aaccaactaa accaaccaga taaaccagat aaactagatc cccgccacgc
     9061 gccgcgaagc catttctgac agcgttattg ttcttcttgg ggaggatcgt tgcttcctgt
     9121 tcggtgcctg aatagaatca cgccggctgg atcttcggat accgcagccg tgctaacaga
     9181 atcggattac acactctcga aggaggacag gcatgtttca taggacctat gggttggtga
     9241 tggccagttt gattgccggt gcgatcggtg ggtttgcggc atcagtcatc atggggacgc
     9301 ccggcgtcgc tcaacagtcc gatcagacca cgtttcaagt cgtgcgcacc agggattttc
     9361 agttgatcga tgcggagggg cggacgcgtg gcaggatagg attctcagca gagacgcagc
     9421 cctatctcca acttgtcgat gagaaagata ttgctggggt atgggttggt gtggccaggg
     9481 aaacaggagt ggccgtgcgt gacacagacg ggaagactcg gctggtcctc agcgtcgacg
     9541 aaacaggaaa tccctcgctc gtcgtccgca accgtcagca tcaaatgcgt tcattccaac
     9601 cgtgatatga gtcagcttct caaggagtgt tgtgagcgtc tcacgcggtg ccgatgctca
     9661 accgctcccg cacggccact gatgtctgct atgagcccca gccacgcgac gggaaaacga
     9721 tcctgacact ggcttctttt cctcattcct tttgctggcg gacagatctc gggcggaaat
     9781 cttcagtgag atggaagatc agcgccggta ccatgtccct gcgaggattg gaatctctca
     9841 tcgcggcgct cacgccttcc ttggccgaca cactgacgcg accgatctgc cttctcgccc
     9901 aggccgaact gttccacacg ctggcacccg atcgggtttc catcagcttc ccggacacgt
     9961 cgcccctgac ctcggtgcgc aacccgccct cgagcctggc gatatcggtg agcctgacgt
    10021 cggtcttcgg ctcggaatac ataatgtcac cgaggaatac cgcggacacg ccgtacttct
    10081 tgccgatctt cgtcatcgcc tctgcgtcga accgagtagc gcccaccgcc tcgagcaccg
    10141 cttcgcggct tcccaactcc aggatcggcg tccccgggta cgccccctgg acatgctcct
    10201 ggaattgctg tgtggcatag ctgttaatgg ccgggtcgga attggaggtg aactcgacga
    10261 tgccgagcgt ctcgtagttc ctcagatcca tacgcggcgg cacggtgacc agtacggtgt
    10321 tggagcatcc ccacaggatt gtgagtgaca gcagcatcgc gaacgcggcg aattgtctca
    10381 tcgtgcctcc tcctccttgt caggcagggc ctcaacagta aagtatagct gaacactcag
    10441 gcagacctgt ggcggggaaa caggaccggg agtcggtctg gcgagtcgta aacgcccctg
    10501 accggtaggc agtctgacag gccagcctga tagggtgagg agcgaacaaa aaggtgaata
    10561 gcccaggcga ttgaccaagg aggctttacg aacaacgggc cagagacccg agccgccacg
    10621 cgccgggaaa tggtttctga ctcccttttt cctgcccatg ttctcattga tggtagctac
    10681 taagccattc tcaaagaaga tcacctttca tatttcagac attctagcta atgatcgatt
    10741 attatgggac tcagacggca ccgttgatgg ttccgttgtc acactgagac gcgtccttaa
    10801 aaataaggag tgaagcatgc gatccatggg tccggttacg attctcatga ctacgttagg
    10861 cctgctgtgg tcggttcagg gttgtacggt tccgccattg atgcaccgag gggggatcat
    10921 ggtggataat tgggctgagg tgaaggcaga cgacaagacc gttgctggaa ttctggccgc
    10981 attcaatcgt gccgaagcgg cacttgaggc tggcaacgtg ccggatttga tgccactcta
    11041 ttcagatcag tatagctatc acggattaaa caaaacagac ttacaaaaag tgtgggagga
    11101 tcttgtctcc caccatcgag aattcagaag caaccatatc ctttcgcgga ttgtgactgt
    11161 ccctggcgat cccctgagtg cagaggtcac gtgcacaggg agtctgtggg ccatttcaaa
    11221 ggaaacggga caacaggtta acattgatag ctggtacgga gaagttcacc acatgcactt
    11281 tgagaatggt gcatggcgtg tgcgcggaca tcgtggagaa gcacccaaga caccggcatt
    11341 tggtgctgca ccacatccat ttttttagtc aaagaaacgc aggaaagaac ggggacaggc
    11401 tgagcgagcc cgccacgcgt cgcgaaatgt tgaagtgcaa gaccggcccc tcaatccggg
    11461 attcagggca atggctaacg ctcggcatca gcggcggcgt gaagcgtcgt ccattgcatg
    11521 ccgttgtgat gccgctcctg actcaagcgc tgttgggcac gagcacgatc ttgcctgtca
    11581 cgcctcctgt cccgagcaac tcgtgcgcgt gccttgcctc ggaaagaggc aggcgctgcg
    11641 cgatgagcgg cttgatcttc tgctgttgaa ggagatcgaa cagggcgatc aaatcctgcc
    11701 gaaacagtgc cggccgcagc cgtttgagcc actgtatgct gtaggggacc acccgtttcc
    11761 gggccgggag aagccagccg ccggcaatat acagcccgaa aatggcgatt gcacgaaagc
    11821 gatgacgacc acctgaatga cctgaagcca atccaccccc acgtagcgac gaggtaatgc
    11881 cataggccac taccgttccg cctgaacgca gagccttgcg ggaacgccag atatgcgtgc
    11941 caccgatgct ctcaaagacg acatctacac catcgctcgt gaggcggtga atttctttca
    12001 cgaagtcctg acgctggtaa tcaattggga caccccccaa gtcggaaacc gtcgacgccc
    12061 ctcgcgatga acaggtaccg tacatttcca gcccggcgag gtgcccaagc tgcaagagtg
    12121 ccgagccgac cccgcctgcc gcgccgtgaa tcagcacgcg ctggccccgt ctggccttgg
    12181 ccgaacgatg cagcatctgg tacgccgtga catagttcag cacgaggctc acagcctcgg
    12241 cggcgtccaa cccggatggc accggcacca gatcacgttg cggcaggcag acaaactccg
    12301 cgtacgcgcc ggtgatcggc agcgcagcaa ctatctggcc tgattcgagt ccggagatgc
    12361 cgtcgccgag tcgatccacc acgccgatca gatcccaccc cggcgtgaag ggcagtgcgg
    12421 gtgtctcggg atgaatgccc tcgcgcatca tgaggtccgg caaggaaaca cctgcggcta
    12481 gcactctcac ccgcacttcg ccgtccttcg gctcggggca ctcttcttcg agcacctgaa
    12541 gtgcatcggg cccgccatag tgggtaacga tgatgcgggt gtgtttcacg gaccgcttcc
    12601 tccctcgccg tcgctccagg ttatgcggcg taactatgga gtatgcagaa ccggcatagt
    12661 acgccgatac cgagcgtcat ggggcctgac accgtgtctt ctgaaggccc tccgtagagt
    12721 tagcggatcg gtgtcggctc ggtctggccg gatgtgcaca tcagactcga tggaatatcg
    12781 agttgccccg atggccggaa tccccacgct gtggcgaaag cctggcaagt caggttccga
    12841 gacgtctcgc cgctgggatc gacacagtgc gggtaatggg cactgcctcg cagtgctgtg
    12901 tcgccacttt ccaccgacag gcctcgtaca ttcacgctta catgaaaaag ggagccgcac
    12961 tgcggctcga tcagcgctcc ttccttactt tcctcgcgtt ccagaaacag tacaaagtcc
    13021 gccttcatct ccttggcgac agcaagtgcc gccttgcggt ccgagaaact gtatgaaacc
    13081 tgcggggtgt ggaaagggcc cttctcaggc atcaggatac tcgagtaatg atgggaccgg
    13141 agccattcca aggccacatc ggccagggga gggtcccccc agagaaggat tcgctgttgg
    13201 gcggttggca agacggtatg agaaccggat gtgatcggga atcggacacc gggtggcggc
    13261 gctttcgagt agcatccttg cacaagaagc gcgccggaga gcagccaaag cacggggaca
    13321 gccagaccgg cgcgatggag tagggagcgc atgacaacgg attctattcc tcgtctccct
    13381 cagtgaccag ggggtgccgt cgggacctga atggaccgtc cagaaaccat tcgaacttga
    13441 tggctttccg ctaaagccag accgcctcgc gccgggaaat gttggcttac aacacctgac
    13501 gcctgagttc tctccggcaa agagatttca tggaatttcc ccagtgtctg caaaatgttt
    13561 taagtcttga acactctcct caaagagcgg cttccagtca gactgcgacg catagtggga
    13621 tggttcgcag aaggacgaat gatagagacc tgccgtcttg ttagcccaat gtttggattg
    13681 ctctagtcca tcccgtgcaa tcaaggtttc aagccgaaga cgttcgctca ttctcaagac
    13741 cggtcccaaa ataccaaagc aaccagacta cgcacataaa ccacatcgac cagatagaac
    13801 ggatcccccc tcatgcgccg aaaaagcggc ctgacaccct ttatttctct tctcgaatgt
    13861 tcactttctc tagaaacccg ccgaggtttg gattggcgta gagaccgtac gcatcagcaa
    13921 tgatcccttt gggtccgcca tcaacctcta tggcatagac gacagacatg tcatccgggt
    13981 cgcttgctcc ctcgaatcga tagtgctcca cgatcgtcag ttcgtcagcc ttgaaagtcc
    14041 ttccgctgtc cacatcccgg aacgattgat tgagaaactc aaaatttgcg gtgaaccctc
    14101 ttttcttcag ctcttgaatg gcttcgacca tattcgtgta aagcttcacg ctcattgaac
    14161 tccctcaaga aacaaagact catcattatt ctactccttt gtcgtgctct attcttatcc
    14221 cgcctgacct cgacggcggt tgctgtctta ggggagtctt ctatgagcgc gcagaggcag
    14281 cgaaaggagc cggaccgcca cgcacagtga aatgaggcct gaaacctttt cgttcccccc
    14341 aggccctgat tcttcaatcg agcgtgaagt taggcaagcc gcaactgcgc caaagaagcg
    14401 aacgcatttc cccgacttat gtccggattg atgttcaatt cgagcacagt gaccgccggg
    14461 agctcgacgt ggtagtcttc cgtctcgcag gttgtgcctt ggggactgaa gttccactgc
    14521 tgccgcacga tttcccggaa ggactgcccg ctatccggtg accaacgcaa gacgtactcc
    14581 tgcgtgcgtt cagttccggg ctccaagaaa tttatccaaa tccgccgcag ccgttgcgga
    14641 tggtggaaga gaagccgaat cgtctgcttt ccgggtcctg cggcacgcca tcccgaagcc
    14701 tgacctggca gcaacgctgc ctcaatagga tgagcggcgt cctccgaggt gatctccacc
    14761 tccgcgagcc cctccacatt caaccaatcc tggtcaggag atgcggtctc tttctgaacg
    14821 tggctgatga tccgcttgcg catcgccttg cctcctggtt tcaagctgga gaaattccca
    14881 gctacgtgac tactaatagg tctactgcag tcagtctact gcaaagtcgg acgcatattc
    14941 atcactatga aggcgatggg gtaggcgagt ctgtcgagtt tgtccggttt gttggtttat
    15001 tttgtgccgc caaccacact gaccagacaa accaaatcaa caagatgaac gaaacgaccg
    15061 ccacgcgccg cgaaatggtc ccgccatgtc aaccattccg gtgactaggg gaaatcccca
    15121 gtattgtata agggaagggg ctggataaga atacatggga aatgttgggc taaattcgga
    15181 aacttctgca tgacgtggag gaggagttat gcctcggctt ccaaccagac ccttcgaaag
    15241 gccaatgccc gaccttgatg agcttgaaga cagcgggcta tccagcgatc accctgcgct
    15301 gcaggatcac ataaaactcg gaacagcttg gttgtgtgtg acagtgacgg gtattggcgc
    15361 gctcgtctac tcgacgattt cgcaattttc ccgaacgtct accatgttgg gtctgatcgg
    15421 gctcctcatt gcctatgcgg cctatggtta ttctctcaga aaaaagaaca cccttcaatt
    15481 tgccgactcg ctctactata tgggttttct ctgggcactc tttgccctta tcgagacctt
    15541 cctggtgtgg cccgctccca aactgactgc tgacgcggtc ctcaccacct ttggctatgc
    15601 gctggtcacg acattttccg gaatgcttct tcgattgttg gtcgttcaat ttcaagtcac
    15661 ggttcccgat cggctggtcc atgcccagga aactattgat cgccgcgttg cggccctcac
    15721 tcaacagctc aacgaagcca cgatggaaat cacgtccttc agagatcgcg cagccagtga
    15781 tctcggtggc acgctccagg atcttgtgcg gtcactagaa gatgttcggg aaacgatcac
    15841 tgagcaacag cggacgatga ccacgatgat gagtgcagga tttgaatctt cgttaaaaga
    15901 tattcttggc cggctcgcag ccatacagat tccccaggaa attctcacga cagaagtggc
    15961 caaactcatt gccgcattag ggaagcgggg agaagacgtt gaagaggcag ttcaaaaact
    16021 ggtgaagagt ttgatgcaag cgaccgatac cgtaacccgc tttggggatt cgttgtatgg
    16081 atcagatgcc gcaaagcgaa ttggagtcgc cgtgaatgat ttgtccagta cgatcaaaga
    16141 tagaaccgaa gaatttgtca aaatgaccac ggcgttggag accagtcgta cagagctgga
    16201 cagtcagctc agtagcctgc agtcattacg atcggcattt gcgaaggtct cgacacaatt
    16261 gtcgacattg gaaacagagt tgagggactt atcgccaagt gcgctaagag ccgatgtcag
    16321 gagcgggctt ctggatgtgc agaagtccat acagtcaagt ctcgacgcga gtagagccat
    16381 cgaatcgaca atgcgcggag tcatgttctt cttgaaggag agagtaactg aggagcatgc
    16441 cttcgatgga aagtgaatcg cgttcaggtt gggcactcgg cttgtcactt gtcgaagtga
    16501 tgctgctgct cgtctttgcg gcgctgatgg tctctgtgac ggacaatgtg gagagcaggg
    16561 gacaagagcc accccaaagc gttgacaaac caaaaaatcc agacgcaatt cttcaaacca
    16621 gactcgatgc ggcgatggag aagagcgagg acttagaaag acagcttaac gaggtgacgc
    16681 gctttgtgaa tgaattgaag ctcatggtgg gcgcgagggt tccaacgaaa gaaggctttc
    16741 acgaagcgat tgaaagctta aaacgcggtt atgccttgtg tcagaaagac aataatacgt
    16801 tgattgaagc aagcgttcaa aatggtgtgg aaacacttca agtcatcgga gagattcctt
    16861 ccgatttggt cgtgaatttt gagaaggggg atcagacgtc ggatcttgac caaattgtgt
    16921 tgttcattca agacgtgttt caatacgaga cagaccacaa gtgtcgattc aattaccgac
    16981 tgaagtatgc aacggacaac gattacagga aggcgagaga aggattcgag aagtattttt
    17041 atcctgagaa aatgatcaga gccggatgat gtggacgtat ctcattacaa gtggaatcag
    17101 atccgccacg cgccgcgaaa ggttccgggc caatttcatt agtctgcatg tttgcgtttg
    17161 agcacgttcg cgagatgggc gaagggcgtg aagggccgat ctggttcacg gctcggcggc
    17221 acctcacccg aggcgtcaga cgccaggtac cgctggtgct cattgtcgtg acagtagagg
    17281 cagagcaact cccagttgct cccgtcaggc ggattgttgt cgtggttgtg gtccttgtga
    17341 tggaccgtga gttggctcag cttcttgccg tcaaattctc gtccgcagtg cgcacagatc
    17401 cacggaaaaa ttttgagcgc ttgctcccga taggtctgcc cccgctgagg ctggttgccc
    17461 agtgccatgc gagcctccgt gaggatggcg actcgtcacg gggatatttt acatcaagtc
    17521 ttggaagaaa gcccgccacg cgcgaggaca ggaggccgga caccgttcag caatgagcaa
    17581 aggttgctga cgccattttc ctcctcttcc cgcaggacat atggactgag ttagtagcct
    17641 ttcgcagcca tgtagctata atgagcacat ggagcggatc aaccgattta cgctggagaa
    17701 gcacgacggc ccatatgaga catggccgct gcattcgcgc ctgttcctgg atggcaagcc
    17761 caccgacctt tccctgccgg ggtacgacct gttgcatcaa ttcgagacat cggattggta
    17821 catcctcatc accgattacg attgtcctta tgaggagatc acgaacttcg ccttgatcag
    17881 caaacacttg agtctgatat cctgtcggtg gctcggctgg atgtacgaaa cattcttgct
    17941 agagcgcatc gagcggattg acgagcgcac atttattgcg gtcattggcg gcagcgatca
    18001 ttgctggcgg ctctctattc ggtcatgggg cattccgtat atccggcccc gtttgaaact
    18061 tcagtggtta gctaatgctc gtagtgattg ccgactcgat tgagcaaggt catcaaacag
    18121 cggcgtccct ggtcggtcag gcgatcaaga agaatccgac cttgcgactc gggttagctg
    18181 caggcaacac accgattggt ttctatcaga gcttagtaga tctgcaccaa tcgacacatc
    18241 ttgatttctc gggggtggaa ttcttcagtc ttgatgaatt ccttgggcta ccccccggga
    18301 gttctatgag ttatgaagcc ttctttcatc aacatttgtt ccgatctgtc aatgctgatc
    18361 ctgctcatat ccatctgtta aaagcggaac ctcacggaga catcttctcc tattgtcact
    18421 cctatgagcg gctgattaat gatcgtggcg gcattgatgt tcaactgctc ggcatcggca
    18481 taaatggcca tctcgcattc aatgaaccga tgtcgagttt caggtctcgc acaaggatgt
    18541 gcctgctgac tcccgagacc agagaaaacc tttcccggac tttcaatgaa acggaggtgc
    18601 ctgaatgggc tgtcacgatg ggcctcggta cgattggcga ggctcagact ctattgatgc
    18661 tggcattcgg taaagaaaag gcaaaggcta tcgcccgagc tgtagaaggg ccattaactg
    18721 ctgcaatccc tgcgtcttcc attcaattgc atccgaatgc cgtcgtgatt cttgacccag
    18781 cagccgctgg ggatctcaag tacaagaact attaccgaca gcaatcatct cgtttggcca
    18841 tgttgctgcc acgcggcctt gcgtagtcat tccattgcgc tggaaaactg tgactggccc
    18901 tccacgcgcc ccgaaatggt tttggctatt ggttcacttt ctaaaattca gcatgtctct
    18961 cttttccggc cacgacagag gttctgggac gtgttctttc catggacctc ggaaggtccg
    19021 aactatttct ggaatgatcc gaaaaagact cccaccccgt tttcttcttg ccggctcaat
    19081 tgacagcttc aagaaatcgg ttggccctat ccgtcgtccg tactaggctt gggtatgatg
    19141 gtcatcagag ccggttggtc gaaacggatg tgggcgttgg cagggatcgg aatcctcctt
    19201 cttctttctg ttgacccctc ctggccgacg gctgaggcaa ctcggcccat tcagattcac
    19261 aaacgtggcg agcagacctt tgcatttcct ggcagttcgg aacgtgacgc agcgccgcgg
    19321 gtaaaccggc cctacttcga tcgggccgga caactaaggt gggccgatgc cttggtgcat
    19381 gaacaggcaa caggctcctg cgatacccat ccattacaag aaaatccaaa tgggtatcaa
    19441 atctatccac gccggactgt tttgctctct cctcgtgttg atgtgcgact gtgcagcagg
    19501 aaaggcgctt catcggagac tacggtgatc ggattgacgg acgatggtca caaggtctgg
    19561 gagcgagaga ccgtctttcg atcgggcgtg catcgcctcg atcaatacct catcggctcg
    19621 tccagggagg gattggtgct cagttcgctc gaggtgtggt cgcccgacac agggcagacg
    19681 attgtgccgg cgtcgactcg gtcgatcaca cctgaatcgc gtgaggttcc gctgtatgca
    19741 tataccggag cggcgttgta tcacccggcg cgccacacgg tttttgtgta cgatgcggac
    19801 gtcacgctga cgaagaagcg cggggggctg gtggaaatca atccgtcgac cggggcgacc
    19861 accctggtcc atccggtcgt aacgacgttg ttcaggggat tcgatcgtat tgaagagatg
    19921 gcgctcatgt ctggcggacg ctatctctgc ctcgcgcaga gactatcctg gcgtggcccg
    19981 acagatgtgt cgttcacaat tcttgatctc gccacgaagc ggctcctgtt tcacgaacga
    20041 ttctgccagg actcgcacga tacctgtgcc gacccccatg tggtcatcgg acccagcgga
    20101 gcgatcgggt tcagttacag taacctgaac aggcgcgagc atcacctact cttatatcaa
    20161 gggagagagt gagatgaaaa tgttcgtggc atgggctgga acaatcgtag gactatgtct
    20221 cgcttcgttc ggctgggccg ggccttcgtc cgaccgtgac gcgatccaca aaacccttct
    20281 acaaagccgt cacctcggtg cccatgggtt gggatataac gcggagtcct atgcagaact
    20341 tgcgaggagg ctctcacccg ctgccattcc gacgttgttg gacctgctcg tccatgacaa
    20401 agaggtacgg gtgggggtgg tcgttggatt agcctcacaa tgcggagcgg cgatcgaacc
    20461 gatcctcaat gcggctgagc gaggcgaagt cccgttcatc agcctgagcg atctgcacga
    20521 tgctcttgcg cagatggaac gcgcagccat ctgttcgagc atggatcaag agcaagcggt
    20581 caaggcgatc atg
//
LOCUS       DATIFR010000107         6912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_261853, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000107 DATIFR010000000
VERSION     DATIFR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261853"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..705)
                     /locus_tag="VLD60_05975"
     CDS             complement(370..705)
                     /locus_tag="VLD60_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB44539.1"
                     /translation="MVFISAGPFTMGQEARPSKNQEKPVVGVDWYDAAEYCQWANKRL
                     PTEAEWEKAARGMDGWVHPWGNDPPTPVHANFEQKAGIGYAALSKVGSFEKGQSPYGD
                     YDLIGSVWE"
     gene            complement(949..1025)
                     /locus_tag="VLD60_05980"
     tRNA            complement(949..1025)
                     /locus_tag="VLD60_05980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(989..991),aa:Pro,seq:tgg)
     gene            complement(1208..1810)
                     /locus_tag="VLD60_05985"
     CDS             complement(1208..1810)
                     /locus_tag="VLD60_05985"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HSB44540.1"
                     /translation="MIHELVLATRNRHKGEELAALLGDLGIRIRTLDEFPGVPDVVED
                     GNTCEANAVKKATEIAAATGLPAVADDTGLEVDALDGRPGVYAARYAGEQATYEDNYK
                     KLLRELSGVPRGRRTARFITVAAIALPWNGVQIATGRLEGLIAEEPAGSRGFGYDPVF
                     FLPELGKTLAELSADEKNRISHRAKAFIQARDLLRSMQLA"
     gene            complement(1821..2564)
                     /gene="rph"
                     /locus_tag="VLD60_05990"
     CDS             complement(1821..2564)
                     /gene="rph"
                     /locus_tag="VLD60_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSB44541.1"
                     /translation="MSGAAGLLRADGRRKDQLRPVKVTRNFIKHAEGSVLIEMGDTKV
                     ICTASVEEKVPPFLKGKGTGWVTAEYSMLPRSTHERSPRESVKGKQGGRTLEIQRLVG
                     RSLRAVTDMSQLGERSIWIDCDVIQADGGTRTASITGSFIALADALSTLKTRGLLKKR
                     PLTDYLAAISVGKVGGEILVDLAYSEDSMAEVDMNLVMTGKGQFVEVQGTAEKTPFAK
                     QDMDGFLAQGWSAIQTLTALQKDLIGALD"
     gene            2999..4141
                     /locus_tag="VLD60_05995"
     CDS             2999..4141
                     /locus_tag="VLD60_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSB44542.1"
                     /translation="MTLLLDRPSRQALLDLKRVLVIDDEEPIRRLLGYMLQSHGYETV
                     MAADAKEARLRLEENPFALVLCDVNMPGESGMDLVRCILSEYPHTATIMVTGLDSSVL
                     ANAALDMGAFGYIIKPFESNEVLINVSNALRRRRLEIENRLHRDNLEDVVRTRTMALQ
                     QALEWLERSEKELRLSREETIQRLAIAAEYRDSATAQHIHRMSHYCELLARRYGLSAE
                     RCDLIRTASPMHDIGKIGTPDHVLLKPGKFTQDEFNVIAQHAEIGYRILAGSDAEILN
                     VAASIALTHHERWDGSGYPRKLKGEAIPVEGRIASIADAFDALTTARVYKPAFEFDHA
                     VELMSKHRGEHFDPELLDVFLASLTDIKRIHEEYADRPPPGLPRES"
     gene            4274..4783
                     /locus_tag="VLD60_06000"
     CDS             4274..4783
                     /locus_tag="VLD60_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44543.1"
                     /translation="MPKKTAAARKSAVTRNWIAYLCEALVTSGAMPTWEAATYLTEHL
                     FGESPNPKLLGTTNAHEITTQFLHRIYHPLVEAASRDVTLPHGAIQHVFTDISLTGKS
                     AVLVVYFPGNGQPHQLLMLDAVKAWDLVFQDSDAFNRWAEDRYRLLKSALTAAIGKPA
                     RQPLLAVVS"
     gene            complement(4809..>6912)
                     /locus_tag="VLD60_06005"
     CDS             complement(4809..>6912)
                     /locus_tag="VLD60_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HSB44544.1"
                     /translation="VATGIEEHNNYAVNFIEATRWIKLHLPGAKVSGGISNISFSFRG
                     NNVVREAMHAAFLYHAIKAGLDMGIVNAGQLAVYEEIPKDLLELVEDVLLNRRPDATE
                     RLVNFAETVKQKGKGVAKDDEWRKGTVEERLSHALVKGITDYIDQDTEEARRKYPKPL
                     SVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLMPYMEEEKKRVGGYQA
                     QGKVLLATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPCEKILATAKDQKVDVVGL
                     SGLITPSLDEMVHVAKEMTREGFDVPLLIGGATTSKAHTAVKIAPSYEPSVIHVLDAS
                     RAVGVVGSLMNREQKPAYVQQVRGDYSRIRQAHHDRGAKPVLPLEKARARRFVCDWTT
                     LDIPVPAFLGPRVISDQLLEQLVSFIDWSPFFHTWELNGRYPSIFEDATIGVRARELF
                     DDAQQLLQRIVKERLLRAKGVYGFFAAASVGDDIELYHDASRTSTLTIIHTLRQQSEK
                     PQGQPNLALADYVAPKGVGRQDHIGAFAVTAGIGLDELCRQFDQDHDDYNSIMAKALA
                     DRLAEAFAEYLHMQVRAEWEYGKAERLTHEELIREKYRGIRPAPGYPACPDHTEKRLL
                     FDLLRVEENTGIVLTESFAMLPASSVSGFYFAHPEARYFAVGKIGKDQIEEYARRKNM
                     PLAAIERWLAPNLNYDPA"
BASE COUNT         1501 a         2128 c         1733 g         1550 t
ORIGIN      
        1 aacctgtcag cgggagggaa cttattcgga aagcggagaa tttcgccgca ctggagcacc
       61 gggatgtcct gctcatctta aattatgact taggtcctga gggtgatggt ctggtgaaaa
      121 ttcggtcatt tgatcagagc atagtcaagg acgaaaggta tgttctgtac cgtaaatcct
      181 attccatgga tgacgcgatt cagcgtcctc tgtgtgtttc tgggaaccac tcgcccccgg
      241 actcaaggta aagaccggcg ttgttcgtac tcttcaccag atgtcctgta agcccattcc
      301 taattttcat ggcaggaaac accgcgccac accatactat gggaagcaca ggctcatgcg
      361 gtctgccact cattcccata cacttccgat caagtcataa tcaccgtaag ggctctgccc
      421 tttttcaaaa ctaccgactt tggagagcgc ggcataacct atccccgcct tctgttcaaa
      481 gttggcatgg acgggggttg gcgggtcgtt tccccaggga tgcacccaac cgtccattcc
      541 ccgcgctgcc ttttcccact cggcctctgt gggtaaccgt ttgttggccc attgacaata
      601 ttctgcggca tcataccaat ccactccaac aaccggcttc tcctgattct tgcttggtct
      661 ggcttcctgc cccatggtga atggcccagc agaaatgaag accatcgacg ccctgtcttt
      721 gtcacccaaa atggctgcga actctgatta ggtataaatg caataccata acgctacgcc
      781 atatatcggc gggcacaagc ctgccaaggg gtttgtggcc tgctgttgtt tcttgtgctc
      841 ctctgtgtaa tccgttcacc gtgactcttt ttggatcgga accgtgacca gaactggact
      901 tgaggagaat gaggaaaaca agaagacaac ggaagtggtt gaagttgttg gtcggggcga
      961 gaggatttga acctccgaca tcctgctccc aaagcaggcg cgctaccggg ctgcgctacg
     1021 ccccgacgtt aggatgttga aaaagaccgc cagcttcgtt ctcgcatcac tgacaggctc
     1081 accgtacagc acaaagtacg actcgcccat tcactcactg cggcctcgct gaacggcctt
     1141 tttgaacatc ctgaattcat gatccacact cagtgccttg ccagaaacct ttttgagcgc
     1201 ccacttgtta agccagctgc atggatcgta acaggtctct ggcctggatg aaagctttcg
     1261 cccgatgact gatgcggttc ttttcatccg ccgacagctc ggccaatgtc tttcccaact
     1321 caggaaggaa gaacacggga tcgtatccaa acccccgtga gcctgccggc tcttccgcaa
     1381 tcaagccttc aagccggccc gtcgcaatct gcaccccgtt ccagggcaat gcaatggctg
     1441 ccaccgtgat gaatcgggca gtccggcgcc cgcgcggcac tccggacagc tcgcgaagca
     1501 actttttata gttatcttcg tatgtcgcct gctcgccggc atagcgagcg gcatacacgc
     1561 ccgggcgccc atcgagggca tccacctcca agcccgtatc gtcggcaacc gccggcaatc
     1621 ctgtggcagc ggcaatctct gtagcttttt tcactgcgtt cgcctcacag gtatttccgt
     1681 cttcgaccac gtctggcaca cctggaaatt catcgagcgt ccggatacga atacccaggt
     1741 ctccaagcag tgcagccaac tcttctccct tatgtcgatt ccgagttgcc aggaccaatt
     1801 cgtgaatcat gtctgccgcg tcagtcgaga gcgccgatca gatctttttg cagcgccgtc
     1861 aacgtttgaa tcgccgacca tccctgcgca aggaatccat ccatgtcctg cttggcaaac
     1921 ggagttttct ccgccgttcc ctgcacctcc acaaactgcc ccttcccggt catcaccaag
     1981 ttcatatcca cttccgccat ggagtcttcg ctgtaggcca aatcgacgag aatttctccc
     2041 ccgaccttcc ctacgctgat cgcagccagg taatccgtca acggccgttt cttcagcaga
     2101 cctcgcgtct ttaacgtgct gagcgcatcg gccagcgcaa taaaggatcc cgtgatggag
     2161 gccgtgcgcg ttcccccatc cgcctgaatg acgtcgcaat caatccaaat ggaccgttca
     2221 cccaattggg acatatcggt gaccgctcgc agggagcgtc cgaccaaccg ctggatctcc
     2281 agcgttctcc ctccctgctt gcctttgacc gattcacgcg gcgaccgctc atgggtcgat
     2341 cgaggaagca tggagtattc cgccgtcacc caaccggttc ctttcccctt caaaaatgga
     2401 ggcacttttt cctccacaga ggcggtacaa atcactttgg tatcacccat ttcgatcaag
     2461 acggaccctt ctgcatgctt gataaaattc ctggtcactt ttacgggacg aagctgatct
     2521 ttccgtcgcc cgtccgctcg aagcagccct gcagcaccgc tcatgaaaat ctcctcctgt
     2581 tgaatcgcgc attatagtga accccctttc aaagcgcaat gaaatcgcct cctggccggc
     2641 actggaagca aactccgata tttcgcgtgc gaccactccg gtgatggtgt gcgtacaaag
     2701 tcgtggggtc ctgtcaaaaa ctgtccgtgg aacggttatc gagaaccgac ggcagtacat
     2761 gacgcacgat gcaccgccag ctcgcaagaa gtccgaaatc aacccccaat cagacttttc
     2821 tcgcagtgat acagaaggcg tagcgttcct ttcaaaaatc tggaaagttg taaaaaatca
     2881 taaattagtg gcacaaaagg tgcagaccat tctagagttc ttcagacaat gcgtgagcgg
     2941 caaaaacgac catctggctc tcgaccgcaa cctgacaata gatagaattg cagcacccat
     3001 gaccttgctc ctcgatcggc ccagccgaca agctcttctc gatctcaagc gtgtgctggt
     3061 tattgacgac gaagaaccca ttcgtcgcct gctgggatat atgcttcaat cccacggtta
     3121 cgaaaccgtc atggcggccg atgccaaaga agcccgcctt cgcctcgaag aaaacccctt
     3181 cgcgctggtg ctctgcgatg tcaacatgcc cggcgagtcc ggcatggatc tcgttcgctg
     3241 catcctgagc gagtatcccc atactgccac gatcatggtg accggcctcg acagctctgt
     3301 cctcgcaaat gcggccctcg acatgggagc gttcggctac atcatcaaac cgtttgagtc
     3361 gaacgaggtt ctgatcaacg tcagcaacgc attgcggcgc cgacgcctgg aaatagaaaa
     3421 tcgcctccat cgagacaatc tcgaggacgt cgtcagaacc aggacgatgg cattgcagca
     3481 ggctttggaa tggctcgaac ggagcgaaaa ggaattgcgc ctctcgcggg aggaaacaat
     3541 tcagcggctg gcgattgcag ccgaataccg ggacagcgcc acagcgcagc atattcatcg
     3601 catgagccac tactgcgaat tgctcgcgcg tcgttatggc ctctccgccg aacgatgcga
     3661 cttgatccgc acggccagcc ccatgcacga tatcggcaag atcggcacgc cggaccacgt
     3721 gctcctgaaa ccgggcaagt tcacgcaaga cgagttcaat gtcatcgccc aacacgccga
     3781 gattggctat cgcattctgg ctggttccga cgccgaaatc ttgaacgtcg ccgcgtcaat
     3841 cgccctcacc catcatgaac gctgggatgg tagcggttat ccccgcaagc tgaagggcga
     3901 agcgatcccc gtcgaaggac ggatcgcttc aattgcggac gcctttgatg cgctcaccac
     3961 cgctcgcgtc tacaaacctg cttttgaatt cgatcacgct gtcgaattga tgagcaaaca
     4021 tcgcggagaa catttcgacc ctgagttact cgatgtcttt ctggcttcgc tcaccgatat
     4081 caaacgcatt cacgaagagt acgccgatcg cccgcccccc ggtctgcctc gagaatccta
     4141 gaactcgttt agttcatttc attgacagtt tttcgctcac cgatatactt caatcagtga
     4201 gtgcagtcgt ttttgcccac cagcaggttc tgtacccaga ccaacgcggt ggcaccagaa
     4261 aggatggctg atcatgccca agaagaccgc ggccgcgagg aaatcggcgg tgacgagaaa
     4321 ctggatcgcc tacctctgcg aggccctcgt gacctccgga gcaatgccga cttgggaagc
     4381 cgccacctac ctcaccgagc acctgttcgg cgaatcaccg aacccgaagc tgctcggcac
     4441 cactaacgcg catgaaatca cgactcaatt cctgcatcgc atctatcacc cgctggtcga
     4501 agccgcttcc cgtgacgtta ccttgcccca cggcgccata cagcacgtct ttacggatat
     4561 cagcctgaca ggcaagtcgg ccgtcctggt tgtatatttc ccgggcaatg ggcagccgca
     4621 ccagttgctc atgcttgatg ccgtcaaggc ctgggacctg gtgtttcagg attcggatgc
     4681 gttcaaccgt tgggcagagg accgttatcg actgctcaag tctgcgctga cggccgcgat
     4741 cggcaagcct gcccgtcaac cgcttctggc ggtagtctcc taactacgct gccgtctgtg
     4801 atggtggatt aagccggatc gtagttcaga ttcggtgcga gccatcgctc aatcgcagcg
     4861 agtggcatgt tctttcggcg ggcgtattct tcaatctgat ccttcccgat ctttcccacg
     4921 gcaaaatacc tggcctcagg atgggcgaaa tagaagccgc tcacggacga ggccggcagc
     4981 atggcaaagc tttctgtcag gacgattccg gtgttctctt ctacccgcag cagatcgaat
     5041 agcaaccgct tttctgtatg gtcgggacag gccggatagc cgggggccgg acgaatcccc
     5101 cgatattttt cgcgaatgag ttcttcgtgc gttaggcgtt ccgccttgcc atattcccat
     5161 tcagcccgaa cttgcatgtg aaggtactcg gcaaaggctt ccgctaaccg atcagcgagg
     5221 gctttcgcca taatcgaatt gtagtcatca tggtcctgat cgaactgccg acagagctca
     5281 tccagtccga tcccagcagt cactgcaaat gctccgatgt gatcctgtcg gcctactcct
     5341 ttcggagcga cataatcggc caatgccagg ttgggctggc cctggggctt ttccgattgc
     5401 tgtcgcagcg tatggataat cgtcaaggtg ctcgtacggg acgcatcatg atagagctca
     5461 atatcatccc cgacgctcgc cgctgcaaag aacccgtaga cgcccttggc tctcaacagg
     5521 cgttccttga cgatgcgttg gagcaattgc tgcgcgtcat cgaacaactc cctggcccgg
     5581 accccgatcg tggcatcctc aaaaatcgag gggtagcgtc cattgagttc ccaagtgtga
     5641 aaaaatggcg accagtcgat gaacgagacc aactgttcaa gaagctgatc gctgattaca
     5701 cggggcccca gaaacgcagg cacgggaata tcgagcgttg tccagtcaca gacgaaacgt
     5761 cgcgcgcgag ccttctcaag cggtaaaacg ggcttcgccc cgcgatcgtg atgggcctgc
     5821 ctgatgcggc tgtaatcgcc cctgacctgt tgcacgtaag ccggcttctg ctctctattc
     5881 atcaagctcc cgacgactcc gacggctcgc gacgcatcca gcacatggat gaccgacggt
     5941 tcgtaggacg gtgcgatctt taccgccgta tgggctttgc tcgtcgtggc gccgccgatc
     6001 aacaacggca cgtcgaaacc ttcgcgggtc atctccttcg cgacatgcac catctcatcc
     6061 agcgacggcg tgatgagacc gctgagcccg acaacatcga ctttctgatc tttcgcggtc
     6121 gccagaattt tctcacaggg caccatgacg cccagatcga tgacttcgta attgttacaa
     6181 cccagcacga ccccgacgat gttctttccg atatcatgaa cgtcaccctt gacggtggcc
     6241 aacaacacct tcccctgggc ttggtagccg cccacccgct tcttttcctc ctccatgtac
     6301 ggcatcaggt aggcgacggc ttttttcatc acccgcgcgc tcttcacgac ttgcggcaaa
     6361 aacatctttc cggatccgaa gagatccccg acgacgttca tcccggccat caagggacct
     6421 tcaatgacgg agagcggctt cgggtacttc cggcgggctt cttccgtatc ctggtcaatg
     6481 tagtctgtga tgcccttgac gagcgcatgc gacagccgct cttcgaccgt gcctttccgc
     6541 cattcatcat ctttcgcgac acctttgccc ttctgcttca cggtctcggc aaagttgacc
     6601 aggcgctcgg tggcatcggg gcggcgattc agcagaacat cttccaccag ttccagcagg
     6661 tccttgggaa tttcttcgta gaccgccaat tggccggcgt tgacaatgcc catatcgaga
     6721 ccggctttga tcgcgtgata cagaaacgcc gcgtgcatcg cctctcgcac gacattgttc
     6781 ccgcggaagg agaacgagat attactgatg ccgccgctga ccttggcgcc cggaagatgc
     6841 agcttgatcc aacgcgtcgc ctcgatgaaa ttcaccgcat aattattgtg ctcctcgatc
     6901 cccgtcgcga cc
//
LOCUS       DATIFR010000108         1998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_836381, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000108 DATIFR010000000
VERSION     DATIFR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836381"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VLD60_06010"
     CDS             complement(<1..1279)
                     /locus_tag="VLD60_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB44545.1"
                     /translation="MTRPNLTMDTTMPDREHPLELWAVWWNAILLLRPAFSRLRTFMW
                     FVTAVAGLTVRVELLGVTSIVRALNLRPRLYTKLLDHFHSSGVKLERLSALWAQVVLQ
                     LFPGIVRVNNRLVLVGDGIKAAKRGKKMPAVKLLHQQSESNTKPEYIMGHSMQAVGLL
                     VHAAKSVFSVPLAVRIHEGLVWSNRDKRSLLDKMLGLLDILAIEAPFYFVADAYYAAG
                     KMVSGLLEQGNHLVTRVKSNAVAYAPAPPKKGRKTRGRPKIYGKKTKLTSLLADINSM
                     QQVPSPVYGECNVTLRYRVRDLLWRPAGRLVRFVAVIHPTRGACILMCTDTSLSAVDI
                     IRLYGLRFKIEHSFKQATRQIGSFAYHFWMKDMIPLRYRNGNQYLHRKSADYRSRVKR
                     KMRAYHTFIQAGVVAQGLLQYLAVVAPKLVWDSF"
     gene            1480..1899
                     /locus_tag="VLD60_06015"
     CDS             1480..1899
                     /locus_tag="VLD60_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44546.1"
                     /translation="MGKQRKEHPQDSIFANNPFLDGLFDWMDSPEGQQCIAVRDVLWD
                     LLEDVQLDAKQRTLIWPDAEPLDLEQSIQRIQKEYPDFPRDEVEEFLLDWIDMGYDPE
                     NYSQAQLDELDSLTERWVADHLRKKKASKKQKRTRHS"
BASE COUNT          432 a          569 c          618 g          379 t
ORIGIN      
        1 cgaaggagtc ccagacgagc ttcggggcga cgacggcgag atactggagc agtccctggg
       61 cgacgacgcc agcctggatg aaggtgtgat aggcgcgcat tttgcgtttg acacggcttc
      121 ggtaatcggc cgatttgcga tggaggtatt gattgccgtt gcgatagcgc aacgggatca
      181 tgtctttcat ccaaaagtgg taggcgaatg agccgatctg gcgcgtggct tgtttgaagc
      241 tgtgctcgat cttgaagcgc aggccataga gacggatgat gtcgacggcg ctcagcgacg
      301 tatcggtgca catcaggatg caagctcccc ttgtgggatg aatgacggcc acgaagcgca
      361 cgagccggcc cgccggacgc cacaacagat cgcggacacg atagcgcaga gtgacgttgc
      421 actctccgta gaccggactg ggaacctgtt gcattgaatt gatatccgcg agcagagacg
      481 tcagctttgt cttcttgccg tagatcttgg gccgccccct tgtcttgcgg cctttcttgg
      541 gcggagcggg agcgtaggcc accgcattgg atttgacccg ggtcaccaga tggttgcctt
      601 gctcgagcaa gccgctcacc atcttgccgg cggcgtagta ggcatcggcg acaaagtaga
      661 agggagcctc gatagccagg atatcgagca agccgagcat cttgtcgagc aagctgcgct
      721 tgtcgcggtt ggaccatacc agaccttcat ggatgcgcac ggcgagcggc acggaaaaga
      781 cgctcttggc cgcatgcacg agcaggccga cagcctgcat ggagtgcccc atgatgtatt
      841 ctggcttggt gttcgattcc gattgctgat gcaggagctt tacggctggc attttcttgc
      901 cgcgtttagc ggctttgatg ccgtccccga ccaggaccag ccggttgttg acccgcacga
      961 tgcctgggaa cagctgcagc acgacttgcg cccacaaggc cgaaagccgc tcgagcttga
     1021 cgccgctgct gtgaaagtga tcgagcagct tggtgtagag cctgggtcgt aggttcaagg
     1081 ctcgcacgat gctggtgaca cccagcaatt ccacccgcac cgtcaagccg gccacggcgg
     1141 tgacgaacca catgaaggtg cgcaggcgcg agaatgccgg ccgcagaaga aggatcgcat
     1201 tccaccagac agcccacagc tcgagtgggt gttcccgatc cggcatggtc gtatccatgg
     1261 tcaagttggg tcgggtcacc caataggcac catagattca caattcgtta accatgctcg
     1321 ggctcatgat ccggacaaga cgagacgagc tacgttgcgg gaatcggggg gcgacgagta
     1381 ttgcgatccc ttgccggcac gatggatatc tcgaagcgcg gcgtcgccgc gcccacacga
     1441 aacgggccgc gatggctcag acctgattga gggcagctca tgggcaagca gcgcaaggag
     1501 catccgcaag acagtatctt cgcgaataac cctttcctcg acggactctt cgattggatg
     1561 gattcgcccg agggccagca atgcatagcg gtacgcgatg tcctctggga cttgctggag
     1621 gacgtgcagc tcgacgccaa gcaacgaaca ttgatctggc cagacgcaga gccgctcgat
     1681 ctcgagcaat ccattcagcg tatccagaaa gagtacccgg actttccccg cgacgaggtc
     1741 gaggagttcc tgctcgactg gatcgacatg ggctacgacc cggaaaacta ctcccaggcg
     1801 caactcgacg agctcgacag cctcacagaa cggtgggtcg ccgaccacct tcgaaagaaa
     1861 aaggcatcaa aaaagcagaa gagaactcgt cactcttgag ttggtctgag tccggtccgg
     1921 gtcatgagcg ccgatatggt cgcccgagca accattcgca tgtccggttc gcccccaatc
     1981 gcgaccgcga cgttgcag
//
LOCUS       DATIFR010000109         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1029507, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000109 DATIFR010000000
VERSION     DATIFR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029507"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..860
                     /locus_tag="VLD60_06020"
     CDS             132..860
                     /locus_tag="VLD60_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB44547.1"
                     /translation="MKRKTSKRSVPAKIVFLFDVDNTLLDNDRVTADLKHHLEKEVGR
                     EGAQCYWNNFERLREELGYADYLGALQRYRSENPHDPRLLTVSRFLIDYPFANRLFPN
                     SLDVVARVKQWGPAVILSDGDVVFQPLKVERSGLFDAVNGNVLIYVHKEKELGNVEDR
                     FPADHYVMVDDKLRILAAIKNIWGRRVTTVFVRQGHYALDPKILATYPAADVSIERIG
                     DLLDHDLAYFTPRTAKGAPDSAIY"
     gene            862..1458
                     /locus_tag="VLD60_06025"
     CDS             862..1458
                     /locus_tag="VLD60_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSB44548.1"
                     /translation="MNPPANPVAFGAPIVLTIGHSTRTLEEFIGLLRFHEATRVVDVR
                     TVPRSRYNPQFNEASLPGSLKKAGFKYVHMPGLGGLRHAKRDSLNPAWHNVSFRGFAD
                     YMQTPEFEQSLDELIQLANRERIVLMCAEAVPWRCHRSLIADALLVRGIRTEDIMGPK
                     RRQAHTLTPFAKVQGTTITYPAEASRTTPKKPSTKRSR"
     gene            complement(1656..1985)
                     /locus_tag="VLD60_06030"
     CDS             complement(1656..1985)
                     /locus_tag="VLD60_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB44549.1"
                     /translation="MGNIIDVDLLEWETVRPDVSHGVYGKTLLNTGVKLVLTRVLPGG
                     GFMAHRDAYGHLLYFIGGSGKIGIGGEEVSIKPGLVVKVETGEEHYYTSSADEELTLL
                     SVNIPGK"
     gene            complement(2127..>2439)
                     /locus_tag="VLD60_06035"
     CDS             complement(2127..>2439)
                     /locus_tag="VLD60_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44550.1"
                     /translation="PKSQKRCVATVRDDKVTVKEVHLGNYTEPLGDFIDSDKAMEVAK
                     KNGLKGNEPSMSVMRPAGARADGTTWIVTGGWKMGVDTTISIEAKTGKFSKRTVIGTD
                     K"
BASE COUNT          531 a          707 c          653 g          548 t
ORIGIN      
        1 atctaaagca gggattgatc tggacaaggc aacccagcaa ctcgaagacg aaggcgtcga
       61 aaagttcggc aaggctttcg atgttttgtt gaaatcgctg gaagagaagc gggtcgcggc
      121 gctacgatag catgaaaagg aaaacttcca agcggtcggt tccagccaaa atcgtatttc
      181 tgttcgacgt ggacaatacg ctgctggata atgatcgcgt aacagcggac ctcaagcatc
      241 atctggaaaa ggaggtcggc cgcgaagggg cgcaatgtta ttggaacaac ttcgagcggt
      301 tgcgggagga actcggttac gccgattatc tcggcgcact gcaacgctac cgcagtgaaa
      361 acccgcacga tccgcgtctc ctcaccgtct cccgtttcct gatcgactat ccgttcgcca
      421 atcgtctgtt cccaaactcg ctcgacgtcg tcgcgcgagt gaagcaatgg ggcccggcag
      481 tcattctgtc cgatggcgat gtggttttcc aaccgctcaa ggtggagcga tcgggactgt
      541 tcgatgcggt gaatggcaac gtattgatct acgtgcacaa ggagaaggaa cttgggaacg
      601 tcgaggaccg ctttcctgcc gatcattacg tgatggtgga tgacaagttg cgtattctcg
      661 ccgccatcaa gaatatctgg ggacggaggg tgacgaccgt cttcgtgcga cagggacact
      721 acgcgctgga tccaaagatt cttgccacct acccggccgc ggacgtgagc atcgagcgca
      781 ttggcgattt gttggatcac gatctggcat acttcacacc acgaacggca aagggtgccc
      841 ccgacagcgc gatatactga tatgaatcca cctgcgaacc cggtcgcatt cggcgcgccc
      901 atcgtgctga cgattgggca ttccacacgc actctcgagg aattcatcgg cctgcttcgg
      961 ttccacgaag cgactcgtgt cgtggatgtg cggaccgtgc cacggtcccg gtacaaccca
     1021 caattcaacg aggcttctct gccaggctct ttaaagaaag ccggctttaa atacgttcac
     1081 atgccgggac tgggcggcct acgtcatgcg aagcgcgatt cgcttaaccc agcctggcac
     1141 aacgtttcct ttcggggttt tgcggattac atgcaaacac cggaattcga gcagagcctc
     1201 gacgaattga tccagttggc gaaccgggag cggatcgtat tgatgtgcgc cgaagcggtg
     1261 ccgtggcgat gtcatcgttc actcatcgcg gatgccctgc tggtccgagg aatccgcacc
     1321 gaggacatca tgggtccaaa acgccgacaa gcccatacgc ttacgccctt cgccaaagtt
     1381 cagggcacca cgatcactta cccggccgag gcctcacgga ccactccaaa gaagccgtcg
     1441 accaaacgct cccggtagca gcctgtcggg aaaactcgac aaaccaaaac gccgaggtcc
     1501 accccataag tccgatgctg gacgacctct gaccagcccc ccgtgatcga tccaccggta
     1561 tcgatgaact ggatactcaa tcttcctctt caagtggcaa ttgtttgcac gtggtttgag
     1621 gtctgtgcgg actctgtcag aatactcttc cggtatcatt tcccggggat attaaccgac
     1681 agcagagtaa gctcttcatc ggcactgctg gtatagtaat gttcctctcc agtctcgacc
     1741 ttgacgacca gcccaggctt gatgctgacc tcttcgcccc cgatgcctat tttccccgaa
     1801 ccaccaatga agtagagcag gtggccataa gcatcgcgat gcgccataaa gccaccgccg
     1861 ggtagcaccc gggttaggac cagtttgact cctgtgttga gcaaggtctt tccatagact
     1921 ccgtgtgaaa cgtccgggcg cacggtttcc cattccaaca ggtcgacgtc gatgatgttg
     1981 cccatgtcta ctccctttca aatggcggtt gtttgcacgg ggtttgctgt ctgtgcggcg
     2041 gtcccttcaa taactcgatt gaggcggatc gtctaaactg taggcggcgt aatggctagg
     2101 gagaaccaat ccaacgtggt tcctattcac ttatcggtcc ctatgaccgt acgtttggag
     2161 aacttgcccg tcttggcttc gatggaaatg gtagtatcaa cccccatttt ccaaccgccg
     2221 gtgacaatcc atgtggtgcc gtcggcacga gcgcccgccg gcctcatgac gctcatcgac
     2281 ggttcgttgc ctttcaaccc gttctttttc gcaacctcca tcgccttgtc gctgtcgatg
     2341 aaatccccga gcggttcggt atagttcccg agatgcacct ccttcaccgt gaccttgtcg
     2401 tcgcggacgg ttgccacaca ccttttctga ctcttgggt
//
LOCUS       DATIFR010000110         1277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_838999, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000110 DATIFR010000000
VERSION     DATIFR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838999"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="groEL"
                     /locus_tag="VLD60_06040"
     CDS             complement(<1..830)
                     /gene="groEL"
                     /locus_tag="VLD60_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSB44551.1"
                     /translation="MPKQLMFDQDARASILKGVNVLTDAVKATLGPKGRNVMIDKKFG
                     APTITKDGVTVAKEIELKDPYENMGAQLVREVASKTSDVAGDGTTTATVLAQAIYREG
                     MKNVTAGANPMDVKRGIEKAVEAVIENLKKMSKLVVDKKEIAQVGSISANSDTTIGDL
                     IAEAVDKVGKDGVITVEEAKSMSTSLDVVEGMQFDRGYISPYFVTNAEKMTAVMDDAF
                     ILLSEKKISSMKDLLPILEQTAKMGKALLIIAEDVEGEALATLVVNKLRGTLQVCAVK
                     A"
     gene            complement(905..1171)
                     /locus_tag="VLD60_06045"
     CDS             complement(905..1171)
                     /locus_tag="VLD60_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSB44552.1"
                     /translation="MSVKFKPLKDRVFVSYTAELEKTAGGLYIPDTAKEKPQSGKVEA
                     VGDEVKSLKVGDKILFDRYSGSKINIDNIEYLIVKEEDVLGILS"
BASE COUNT          233 a          389 c          320 g          335 t
ORIGIN      
        1 gccttcaccg cgcagacctg aagggtgccg cggagcttgt tcaccacgag cgttgccagg
       61 gcttcgccct cgacgtcctc ggcgatgatc aggagcgcct tgcccatctt ggcggtctgc
      121 tccaggatcg gaagcaggtc cttcatgctc gagatcttct tctcagagag caggatgaac
      181 gcatcatcca tcaccgccgt catcttttcg gcgttggtca caaaataggg ggagatgtag
      241 ccgcggtcga actgcatgcc ctcgaccacg tccaggctcg tggacatgct cttcgcctct
      301 tccacggtga tgacgccgtc cttgccgacc ttgtccacgg cttcggcaat gaggtcgccg
      361 atagtggtgt cgctgtttgc cgagatgctg ccgacctggg cgatctcctt cttgtccacg
      421 accagcttgg acatcttttt caggttctca atgaccgctt ccacggcctt ctcgatcccg
      481 cgtttgacgt ccatggggtt cgcaccggcg gtcacgttct tcatgccttc gcggtagatg
      541 gcctgggcca gcacggtcgc cgtggtcgtg ccgtcgccgg caacgtccga ggtcttcgag
      601 gccacttcgc gcacgagctg cgcgcccatg ttttcatagg ggtccttcag ttcaatttcc
      661 ttggccacgg tcacgccgtc cttcgtaatc gtcggcgcgc cgaatttctt gtcgatcatg
      721 acgttgcggc ccttgggccc caacgtcgcc ttcacggcat ccgtcaggac attcacaccc
      781 ttcaggatgg acgcccgggc atcctggtca aacatcagct gttttggcat aacttttttc
      841 ctcctctggt attcttcgat aacgttgggt aaattctgct ctcaagcggg ttttcgcggc
      901 gcacttagct caaaataccg agcacatctt cttctttcac gatgaggtac tctatgttgt
      961 cgatgttgat cttggagcct gaataacggt cgaagagtat cttgtcaccg accttgagag
     1021 acttcacctc gtcgccaacg gcctctacct tgccgctctg cggtttctcc ttggcagtgt
     1081 ccgggatata aaggccgcct gccgtctttt ccaactctgc cgtgtagctc acgaagaccc
     1141 tgtccttcaa tggcttgaat tttacactca tacaattaac tcctttctgc gatggaatat
     1201 tttaaagtat atcagaacat tagcactcat tcaaggcgag tgctaatata tttcccgacc
     1261 gcaaaaaaag caaaccc
//
LOCUS       DATIFR010000111        45594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1605000, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000111 DATIFR010000000
VERSION     DATIFR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 45594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45594
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605000"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VLD60_06050"
     CDS             <1..375
                     /locus_tag="VLD60_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44553.1"
                     /translation="MKGHVMKILGLCLLLILPATLCWADSESQKPEEQKSEDQKPEEQ
                     EPTLYDHYHAMKQLQEAAASRTIDEQARFQPLIQREQRLACERLRKDHRKRVQKEEYR
                     RQGGDEFLVFSWELEQWCGTLR"
     gene            509..1711
                     /locus_tag="VLD60_06055"
     CDS             509..1711
                     /locus_tag="VLD60_06055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSB44554.1"
                     /translation="MLELQSRGSCSRSSLKDSAFTHVPAVDGLRGIAILLVLWYHAPF
                     LFRDLPEFGINHSPWQALGVAGTMSLGGWIGVDLFFVVSGFLITSILLRLRDWGGSSW
                     VFWGRRGVRILPLVMLYLLILFALAWLGDPLKMLPHFEGWAWYALYLGNIHITLYGWQ
                     PLAVMILWSLAIEEQFYLVWPLVVRACSRQRLLWCGAGMIAVAPVVRAIMLSTSDYPA
                     TYVFTLCRLDALAAGSVVAVIFGSDTWKSEAACLCKRLAPFALVLILMTFLVPFSPSL
                     PETRPWFFSVFGYSWLAVSYAILLGASLNADGIFGAILKSPVLTILGRRCYGLYLWHV
                     LIAGFVIAGLQLFQLGFYVHMVLWLGTLLIVASGSWRFFEEPILRLKQFLPYGDNESE
                     RLESKLVA"
     gene            2110..2670
                     /locus_tag="VLD60_06060"
     CDS             2110..2670
                     /locus_tag="VLD60_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HSB44555.1"
                     /translation="MKKGLWGLTILAWSCSAALVGVMAEESQAAQKKAKEQASVVSAK
                     SPAPVPELSQKPSNANGKKAAASVVLERQSEVLQQQAKQTMSEVKPTTQKGHRRINVH
                     KKHAPKAVVQPRQDLAHHGLLEDSQRYDPRLNHRTAGVQNPQTPDLTYDHFQELDRNQ
                     DGKIDPVERAFARLDMDRDLHDRHLQ"
     gene            2862..3596
                     /locus_tag="VLD60_06065"
     CDS             2862..3596
                     /locus_tag="VLD60_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HSB44556.1"
                     /translation="MIREQTMPNDITAAQPASGSSLGKIAIAVMIVCGIGAFVYFDLG
                     RFLSLETLKENRDHLLAFTEANYAASVVLFILAYIAVTGLSLPGAVILTLAGGFLFGS
                     VFGTLFVNLGATTGATLAFLAARYLLRDWVEQKFGKWLGPLQQGFTANAFSYLMTLRL
                     IPLFPFFVVNLVSGLTRMNIGTYVAATALGIIPGSFVYAYAGRQLGTINSLKEIASPN
                     VIAAFVLLGLLALAPIVYKKFTAKQA"
     gene            3686..5239
                     /locus_tag="VLD60_06070"
     CDS             3686..5239
                     /locus_tag="VLD60_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HSB44557.1"
                     /translation="MRQHEEGLVLPNDEHNRILAENVHPSDWVNPEPTGRYNIVVLGA
                     GTAGLITAVIAASLGAKVALVEKHLMGGDCLNVGCVPSKGVIRAARAWADLRNAAEFG
                     LHIPPGVKYDFGAAMARMRKLRARISHNDSAHRYTKLGVDVYIGSGRFTAADTIQVEG
                     SAGSRTLTFAKAAVCTGARASSPPTPGLKEAGYLTNETVFSLTELPQRLGVIGAGPIG
                     CELAQAFARFGSQVYLIEALHGIMPNEDRDAADIVEQQMVRDGVKLLCCGKDLKVQKA
                     EDGKRLTVDSHGQQYDVTVDEILVGVGRTPNVDGLGLEAAGVEYDKNGVMVNARLQTT
                     NPRIFAAGDICSRHKFTHAADAMAQIVIQNALFPHPFGFGYASVESLTMPWCTFTEPE
                     VAHVGMYEKDAKEKGLEVETYTYKLDEVDRAILDGEDAGFARIHIQKGTDKIVGATIV
                     AAHAGEMISEFSVLMKAGAGAKTLAGTIHPYPTQAEVNKKVVNLWRKAHFTPRTKSLL
                     TKLFAWLRR"
     gene            5253..6005
                     /locus_tag="VLD60_06075"
     CDS             5253..6005
                     /locus_tag="VLD60_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSB44558.1"
                     /translation="MTGARSVHRAWLFGLGLLIGPICLWAQDPPLLEVAKFSAGQVGT
                     GLPEGWKPLTFKKVPKQTAYELVKDGDVVVVKAVSQASASGLTKEVKIDPKEFPIIQW
                     RWKVENLLKHSDVARREGDDYPARLYITFEYDPEKVSFGKKLKFKAGQAIFGDIPIGA
                     LNYIWETNAPVGAILDNAYTDFAKMVVVESGPQKVGLWVDEARNIYEDYKRAFGEDPP
                     MINGVAIMSDTDNTKETAIAYYGDIQFVRAKK"
     gene            complement(6108..6500)
                     /locus_tag="VLD60_06080"
     CDS             complement(6108..6500)
                     /locus_tag="VLD60_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44559.1"
                     /translation="MKKAKKKKILPVIVAALAVTALAVGPAVAKNSKKSSSHSSAAAA
                     ASSMGSIPQAPDAPKAGRHDEKPGPAWRTVGGTVKQIENDAYLVEDYEGNQVKLYVGQ
                     GTKHINKKKVGDTVRAEITRGGFANSIQ"
     gene            6757..7563
                     /locus_tag="VLD60_06085"
     CDS             6757..7563
                     /locus_tag="VLD60_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HSB44560.1"
                     /translation="MGSIMNRSVSLGLAFILAGCASVPTSFHPRDPISPPGFSHHAFD
                     AVVRSAVSNGQVNYRAIQSDLRFRQYLDQLDRIDPNGFSREHRLAFWINAYNAFAIKG
                     ILDGESPAPYVGWYRYFKARDYAVGGARVNLYDLEHGILRKQFNEPLVHFAIVCASAS
                     CPKLQPWAYDGATLDRQLDEAVRGFINDSARNRFDRQQKVASLSMIFKWFESDFAGAA
                     GSVLAFIAPYVNDPELARELSQPGYRIEYLEYDWSLNGLPPEESAYARTS"
     gene            7547..8251
                     /locus_tag="VLD60_06090"
     CDS             7547..8251
                     /locus_tag="VLD60_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44561.1"
                     /translation="MLVRLDEKVPIARLLTFLEHGERMANECAKAQAGFALDAGSRRF
                     LITQARQESAHAMVFQGAIAWLAPRHLGDAPFLPALEEYRSILNEALIRQDLCETFLA
                     EQVILEGLGEAILTRIEEGLVKRAAPFGRLRRILLQQEEAHHGFGRRMLERATAEGRT
                     DAETLRRRAQDYLALTDQMVLTLSDLFESIAEDPTAWAKDVRKFLPEWLHQEEQSSFN
                     NQSSALGCSEKLIAER"
     gene            8269..8979
                     /locus_tag="VLD60_06095"
     CDS             8269..8979
                     /locus_tag="VLD60_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSB44562.1"
                     /translation="MISVVIPAYNEEKALPETLRALFRQPGDYEVIAVDGGSTDRTLA
                     VLSEFGFERPSPSSPHPSRQLLTARKGRASQMNAGALQARGEWLLFLHADTTLPPEAI
                     QRLNEMDLDLTIQAGGFTHQFSGNDWRLKMISFLDNFRCTRSRIIYGDQALFVRRALF
                     KRLGGFPDQPILEDVAFCERLIAGTTPVLLSPPVITDARKFLKMGVWRSFLRVLLIIL
                     HVEFRLPVLPRRFFQDVR"
     gene            9208..10527
                     /locus_tag="VLD60_06100"
     CDS             9208..10527
                     /locus_tag="VLD60_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44563.1"
                     /translation="MRTMTNQVKRTFLLERLPARHLGMLRQIGRLADEQDLPVYLVGG
                     VVRDLLLKRTNWDLDLTVEGDGIAFARLVADRYRAGLATFERFGTARLVMPNGIRVDI
                     ASTRRESYMRPAALPNVEPATLLEDLRRRDFTINAMAIQLNSATFGRLHDPYGGQRDL
                     RARTIRVLHEGSFVDDPTRMFRAIRFTQRFGFRLESQTAQLLKAGAATDLIAQLSGPR
                     LCSEILLLAGEVFPQRSFDWLRRLKLLRFLHPDLRYTGPARRIVQSLPSALAWWRTRC
                     DGNSVDRPLVYLMALLGGAGQPIIEAVVTRLMLSNEQGRKVRAGGQRLKSVVCGLGET
                     RRLRPSEVHHLLNGLPDEALVLCLAMVKKRTAMLARVKRRIRDYVQRLRATKPALGGD
                     DLLHMGVKPGPAVGTILALLLEAKLDGVAKGKAGERAFVRARLAGRG"
     gene            complement(10797..11468)
                     /locus_tag="VLD60_06105"
     CDS             complement(10797..11468)
                     /locus_tag="VLD60_06105"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HSB44564.1"
                     /translation="MDNNQTPRALSHDEKNAAEAAFAGKPLNPKWSARARTVYEGIIK
                     VLPAPSEIPVDASKISEEVAPTSDLAPPESVTEPEGSTAEPSTEGVEQTGNVPTKIPF
                     KQALETGALIDVTPTAKQLGFSFPVTVTKPLWDVGIAPVKTLSQEDQANRLRDVLMAF
                     RLRLATQPTVSPLIDFPALLAFPPNSVPQPIPLFALIQPDEENRATVTLLLPNEVAAS
                     IIPLN"
     gene            complement(11518..12786)
                     /locus_tag="VLD60_06110"
     CDS             complement(11518..12786)
                     /locus_tag="VLD60_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44565.1"
                     /translation="MSGQSWRSGVTRYQWLVLFVAWLGWVFDAMDATIYAIVLHPALR
                     ELLQTSAGATVLPEEIGWYGGIIFSIFLIGWAIGGITFGILADRFGRTKILIATILIY
                     AVFTGAAALSQDWWHLAIYRFLTALGIGGEWAAGAAIVAETWPEAKRAKAAGILQSAW
                     AVGFFLAATFNLTLQDAYGWRGLFLIGILPAFVALLVRWWVKEPERWTHAHEQQVLPL
                     TAIFEGNLRRGTLVGSALAFVAVFGLWGSTNWAPTLIRELPDLNGQDSATLSKYVSYA
                     IMALNAGAIFGYLGFGPLADVFGRRPVFAFMCLGSFIMLPVTYLLPSTYAGVLMLLPI
                     LGFFNNGIFSGFPIYLPELYPTVLRTTGAGFCFNAGRVLASVAPFVTGWLVTTLGTFG
                     RAASTVASIYLLGLLVLLFAPETKGKPLPD"
     gene            12938..13576
                     /locus_tag="VLD60_06115"
     CDS             12938..13576
                     /locus_tag="VLD60_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSB44566.1"
                     /translation="MARVDFYRVLGVSRESSDETIKKAYRKLVFEHHPDRNPDSKEAE
                     SRIREINAAYEIIGDPESRRNYDRLNWGDEFREDVADLSVILDEMEKKLFDEGRKELF
                     AVLVKEVARIKAELALIRERTVADQGYDSFKEDLIHARAAEVMHEFVTDEMERRKQRL
                     VDVATQMMVSQGVVGKNDEGGIRSLRGRLDEQFCKGRLHGFASALELFYERR"
     gene            complement(13659..14867)
                     /locus_tag="VLD60_06120"
     CDS             complement(13659..14867)
                     /locus_tag="VLD60_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB44567.1"
                     /translation="MAIAIHNTRLIDGNGQAIERATVIVRSGKIHAAGPSRAVSIPRG
                     ATRIDGRGLTLLPGLIDCHVHLCLGAEPDVVQAIEQETPTETLLKSSQMARQTLEAGV
                     TTVRDVGSRDHSIFALKRSIDKGLTAGPRIVGAGLAICMIGGHARFIGQEVEGVEQVR
                     KVVQAQIAAGAQVIKVIASGGVLTPGTSPDQAQLTLDELQAAVAEAKRAGLKVAAHAH
                     GASGMKNAVRAGAHSIEHATLMDEESAALMNEQAVYMVPTLSALATTAACRRGCGIPD
                     SALDKAKAMTKRHQASFKQAHRRGLLIAMGTDAGTPFNYHGDNAQELERMVTFGMSPM
                     EAILASTSSAARLIGIQDIVGTIERGKEADLILIDGNPLRKIELLRDRGRIVGVMRSG
                     KFVAGLLSKD"
     gene            complement(14872..15468)
                     /locus_tag="VLD60_06125"
     CDS             complement(14872..15468)
                     /locus_tag="VLD60_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44568.1"
                     /translation="MNNKPLLWALGGGAFAFVAVVSYWIFALTLANQMKSDQVSPEKT
                     ASFIHAILEANRNNYTDNVVVKLRRDGVEAHEHWRDERGVPLPAQFLLESGRLVALKD
                     LNFSFRLASLTPIYVWNGANSDLERVGLAAVQKDPSRPHYGLIRKGELRLFQAIYADR
                     AVSQACVDCHNQHPNSPRRDFKLDDVMGGIVITFPIGE"
     gene            complement(15488..16618)
                     /locus_tag="VLD60_06130"
     CDS             complement(15488..16618)
                     /locus_tag="VLD60_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSB44569.1"
                     /translation="MVHPRVLRALSAPLLGHLDPVFLTVMNDVQTLLRYVFQTSNRFT
                     IAISGTGSAGMEASIVNFVEPGDAVVVGINGVFGGRLASVIERCGGKAIRVEAPWGEC
                     LDPSALDQALRRSGPVKAVAVVHAETSTGAWQPLDDIAKICRQHGALFVVDAVTSLGG
                     VPVDVDRLGIDVCYSGTQKCLSCPPGLAPFTMSDRALAVVKQRRTPCQSWYLDMSLIA
                     DYWAEGKRAYHHTAPISMLYALREALRLVEEEGLPARFNRHQMNSDALVAGLEELGLT
                     PLPRIGQRLPVLNCVTVPSHILEAEVRAELLTNYGIEIGGGLGPLKGKVWRIGLMGES
                     STEAHVLTLLNALESLFIRGGWLTTPGIALQAASRVYSHVNL"
     gene            16816..18318
                     /locus_tag="VLD60_06135"
     CDS             16816..18318
                     /locus_tag="VLD60_06135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB44570.1"
                     /translation="MDDFVTPSSQPPSSRSWWRETEAPPAVGQFPARFSPLISWLAAI
                     FLVASFAAVVVMASSTPKIDRIEAPERALSLMVGRTMEVQEGLKRAPAWEQEWFGWVS
                     GNGEAEQAYAIEWYRELSKHSDDPVVPLDLAILQAETGQISQALLSAHEWDEREDPLP
                     RFAELIRAAYSEDLPPDRETIEAMQADLPELLPQGWFYDRLAIRLAQRSNNPELVARL
                     QEQAAIQADKLYARSRKLTLFELGAMLLGTLLLLFILHGRAADANPIRLHEPGIPPPW
                     PGRVGAAVLLRGGAIGAVVMAVCLMYLPPENASLRALAIPMTNVPLLALAYYHLFRPA
                     GMTFEEGFGLSLPWGHFGRLLVAVLAVVAAGLWGEWVMARLSEQWHLASHWTEWFDGD
                     LVWGSPSVTMVSLLEYVIFAPLFEELAFRGLLFAILRRKFSFLPAALISAGIFGLAHG
                     YGAVGLISVCWSGVLWAWIYEKTGSLAPGILAHAINNLLVCLAVMALLRW"
     gene            complement(18323..19111)
                     /locus_tag="VLD60_06140"
     CDS             complement(18323..19111)
                     /locus_tag="VLD60_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44571.1"
                     /translation="MDNRALVILGSGYTGRFLAPRAARQYPYVFVTSRSPEQHLPDVP
                     AQQRIEFDLERSATWAHIPPAADLLWCFPATPLELVHQFAEAIKAPSRRIVLLGSTSA
                     YDVGDSHNYPPAWLDESAPIDRSKSRVQGEEFLRTQCHAIVLRVAGIYGPGRNPLDWI
                     KNGRVGPSRKYVNLIHVDDLVVTCLAALERGKPGETYNVSDGHPHTWEVICSTAHERW
                     NVPLRTESQADASGKRINTHKLTTELGVRVQHFDLFTALEGLER"
     gene            19274..20713
                     /locus_tag="VLD60_06145"
     CDS             19274..20713
                     /locus_tag="VLD60_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44572.1"
                     /translation="MTSMRSLRFLAAACLMLAGCNNGGDVTGGSINLSASGAVVYLAD
                     QNTVGVFELYLASSGTKLTPPLTPGGDVISFALTPDKTAVVYIADQDTNDVFELYKVT
                     IATPGVSVKLSGTLVSGGDVSSFAITPDGTSVVYLADQQIDQVFDLYRVLFSNPASST
                     LLNPALPSGRTVTQFAVTPDSTRVVYIADQTTDNVNELYQVAFLNPPSDNTKLNSSLV
                     AGGNVVNFAISPNSASVVYLADQTIDQVFEIFLAAVTGPGSGTSLNPPLVAGQNVTTF
                     AVTPDSTAVIYRADEDTTGTFELYRVLFLSPQVSVVKLNGTLTAGGNVSSFSIAANGS
                     AVVYSADQTILGVVELFRTPLAPVGPAQKVNPTYVAGQNVTAFAITPDSSAAIYMANQ
                     TSAAVQLYQVSFSSLQVSSPPLNGALASGGNVASFSISPNSTLVLYLADQTTFGTEEL
                     YSVNVGAPGTSSKLNGPLVLGGNVTAFTF"
     gene            20809..25317
                     /locus_tag="VLD60_06150"
     CDS             20809..25317
                     /locus_tag="VLD60_06150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB44573.1"
                     /translation="MPLTRFHPLIAEWFQSHVGQPTDVQLQAWPAIQSGADALIAAPT
                     GSGKTFAAFLSCIDYLFKQALGRELDDHTQILYVSPLKALSNDIQKNLQKPLADIGQA
                     ALQAGLLMPELRVLVRTGDTPMADRQQMLKRPPHILVTTPESLFILLTAEKSRRLLQT
                     VRTVIVDEIHALAPNKRGAHVALSLERLEALTLVKPQRIGLSATQQPIELVAQFLVGA
                     GVRSSKCNVRSSPIPETSNGEPRISISIAPCRIIDVGHRRKMDVAVEVPKDELSAVAT
                     NAIWSDIYDRVAALVREHRTTLVFVNTRRLAERVSHHLEERLQDLGADVVAAHHGSLS
                     RQIRLSAEERLKSGKTRVVVATASLELGIDVGTVDLVCQIGSPRSIATCLQRIGRAGH
                     WIHAIPKGRLFATTRDELLECAALIRAMKGGQLDHIVVPPEPLDILAQQIVAAAATQA
                     WTEQDLFTLVRRAFPYRDLDRATFDAIVQMLADGIATNRGRGLAYLYHDRINHRLKGR
                     RGARLAAITSGGAIPDTANYAVVAEPDGTVVGSVDEDFAVESLAGDIMLLGNTSWRIK
                     GVEMGKVRVEDAHGAPPNIPFWRGEAPSRTAELSAEVAALRQEIAQQASGTRQEASGD
                     ETVPRAPLASRLSPLAWLKEECGLDQRGAQQAVEYVLAGRAILGTVPTQETIVAERFF
                     DESGGMQLVIHAPFGGRINRAWGLALRKRFCVTFDFELQAAATDNGLVISLGEKHSFP
                     LESVFGYLHSNSVREVLIQAVLLAPMFATRWRWNASRSLALLRFSNGKKVPPQIQRMK
                     AEDLLAAVFPDAIACQENLTGARATRQIPDHPLVQETLRDCLTEAMDIDGLIDVLRRI
                     EAGTITCVAIDTPAPSLFSHEILNANPYAFLDDAPLEERRARAVELRRTLPADVAAGI
                     GALDPEAIEEVARESWPVVRDADELHDALLTLVWVPSSEASEWSAYVPRLVEEGRVVE
                     LSVRGEGLGVRGDGREVKGWVARENQESVEQLYVRGSEPTLDAIVQGWMESIGPTTTG
                     ELAERLHLSSDAVNESMVRLESQGQVLRGQFRERRGEASGVRREADGISPSPSPLTPY
                     GVEWCHRRVLARIHRLTIGKLRKEIEPVTAAEFMRFLLQWQHVASGSRHHGESGLLEV
                     IKQLAGFETAASSWDTQVLRVRMAKYEPELLDRLCLSGAVSWGRLSPHPRLAQSDGPE
                     RAKRIIPTSVAPISVFPREDSEWLLDTFQDGTGQGPEPFAALGAVAQDLRRALQERGA
                     CFFADLVRATGHLASEVEEGLWELVAAGLVTGDGFDNLRALMDPHRRRAEGRERTRRP
                     RHAAGRWSILRNASSHQPSAVSSGSVSPVTLRLTPYASRSVELVARQLLRRYGVVFRD
                     LLGRETLVQSWRDLLVAYRRMELKGEVRGGRFVSGFVGEQFALPEAVEALRAIRKGGG
                     GTSGLEVKLSACDPLNLAGVILPGPRIPAVPTNFLVFKDGVVARTVLGREIVEQSDST
                     VAWARRHD"
     gene            complement(25345..25737)
                     /locus_tag="VLD60_06155"
     CDS             complement(25345..25737)
                     /locus_tag="VLD60_06155"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HSB44574.1"
                     /translation="MKQLRTYCLMFAAMTALLMISAGCTLKGTTNETTDTTSNITGTT
                     SGRIWWNEDGLLNPEHKAIAFATYNEANLEQDLARGQGEYLTSMGTLLGVAPEAQPTF
                     QAAAQDRFAQLKTADQQARLEQLRTLSR"
     gene            complement(25884..27233)
                     /locus_tag="VLD60_06160"
     CDS             complement(25884..27233)
                     /locus_tag="VLD60_06160"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HSB44575.1"
                     /translation="MPNRRFYVLALALLVIFLVGNGTAFAFCGFYVGKADTKLFNKAS
                     EVAIARHDNKTVITMANDFKGEVKEFAMVVPVPTVLEKEQIHIGNPAVLKHLADYSAP
                     RLVEYFDENPCIKYELMERKRDAMKNMAPASAAAPERDRALGVTVEAQYTVGEYDILI
                     LSAKESAGLETWLSENGYRIPNGASSVLQSYLKQGMKFFVAKVNLAEQSKLGFTHLRP
                     LQIAFESPKFILPIRLGTVNADGAQELFIYFLTKQGRVETTNYRTVRLPEAQEIPLYV
                     KDKFGDFYRDLFTQQVKRENERGVFLEYAWDMNWCDPCAANPLSAEELRSLGVFWQDN
                     AGRMGKMMPVAQNVFLTRLHVRYDAAHFPEDLIFQETSDRGNFQARYILRHPWTGSDE
                     CPTATAYRQQLRERYEREAQTLATLTGWNIGEIRKSMNIASLPPVEEKKWYQRLWSN"
     gene            complement(27515..28414)
                     /locus_tag="VLD60_06165"
     CDS             complement(27515..28414)
                     /locus_tag="VLD60_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HSB44576.1"
                     /translation="MGQDNGKSQPNTPANWRDPRLYQIASLSTLLLYGLGWLHFDVSP
                     WQIAITFGTALLTQYVGTRYFKLRAFDPRSPLISALSLCLLLRTNDLVVAALASFIAI
                     GSKFIIRWKEKHLLNPTNLALVVVLAGGLGWISPGQWGQVAWFGFLIACLGSLVVTRA
                     ARADVTLAFLTFYVGLLFTRSIWLGDPLTIPLHQIESGALLIFAFFMISDPKTTPDSR
                     SGRILFALFVALAALYVQFGLFRPNGPLWGLIVCFPLVPLLDYLLPGVRYDWSKPSSG
                     KLSVSISLPVLVPLSISQQRRIS"
     gene            28937..30109
                     /locus_tag="VLD60_06170"
     CDS             28937..30109
                     /locus_tag="VLD60_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44577.1"
                     /translation="MRQAFIIMQIGNAQLDAICKDAIVPALAACGFDAKRVDKHNEGG
                     LLKSEIIGFIERSEIIVADLTNERPNCYLEIGYAMGIEKFRNLVLTVREDHNPESPSY
                     VRGGPKIHFDLAGYDILFWHPDNLDAFRAELEKKVRRRLAVVASPPAVPALSWDEEWI
                     AAQRQVAMKGLSAAGKKAFMEARFALEVPKPNKTQRELDEAARQAEIHTFGWPIGAYL
                     GNRADCRPRPRADGIVAELAFKDRSSYDYWAVRRNGDFYFLGSLFEDERDPSKIFFNT
                     RIVRVTETLLYCARLYSRLEIDPTVFVNVAVRHGGLKGRFIGSSSPGRILSPRAASTE
                     DEIDCEVRTSLQEIESNLVPLVKQLVAPVFVLFDFFELADSVYEHIVNSFVDGRVV"
     gene            30224..31042
                     /locus_tag="VLD60_06175"
     CDS             30224..31042
                     /locus_tag="VLD60_06175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSB44578.1"
                     /translation="MAFEDLIRIGSYLSVLGVMATWELLAPRRMLTASKLCRWGGNLT
                     IVILNTAIARLLFAGGVAATAAIAQERGWGLLNWVDAPTWLEFGLAVVALDLVIYGQH
                     QVFHGVPILWRFHMMHHSDLDLDVSSGVRFHPVEIIISTVVKGAAVVALGVAPLAVVA
                     FEIVLNSTALFNHSNVKIPLAFEPILRWFVVTPDMHRIHHSVDPRETNSNYGFNVPWW
                     DRLFGTYCAKPALGQQGMKIGLEHLGPPVCLNLFMMLQFPFVAQLGRYAGRTQP"
     gene            complement(31086..32546)
                     /gene="pyk"
                     /locus_tag="VLD60_06180"
     CDS             complement(31086..32546)
                     /gene="pyk"
                     /locus_tag="VLD60_06180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSB44579.1"
                     /translation="MMRKAKIVCTIGPASNPPAMLDRLIESGMDVARLNFSHGTHESH
                     AAAITAIRYAAERRRAAVAIIQDLQGPRIRVGTVADEGIEVRAGQQVHLQTILARSGG
                     QLGVQSVAIARSAVPEIPVTYQYLARDIQPGARILINDGLIELIADRITGGSVECTVT
                     IGGRITSTKGINLPGTRVSAPTLTDKDREDIRFGIAQGVDYLALSFVRGPEDIVAARK
                     LVAECGGNLPIIAKIERSEAVANLDEILEQADGVMIARGDLGVEMGPEAVPVLQKRII
                     AEANRGRRLVITATQMLESMTSALRPTRAEASDVANAVFDGTDAVMLSAETAIGSHPI
                     ETVQVMDRIIRAAEEGAKPGWLPKADGEQGELSFPEAICTAASSAARTINAGAIVAFS
                     EQGMTARLISKQRPAAPIIALTPFEPVRQRMALYWGVRPYTMPQIEQTDARVDEAERR
                     LRTEGLVKTGERIVILSGTRIGQPGGTNLIKLHEVG"
     gene            32662..34989
                     /locus_tag="VLD60_06185"
     CDS             32662..34989
                     /locus_tag="VLD60_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSB44580.1"
                     /translation="MQINTDPLDFVTIEGLLAYGEEFARRPSQDGVLLPPAPPPSNDR
                     AGRARAAMEKIAAFVQTAQGGFPGADTYRAARKAVIDEACAGDDLVFFAAWNRLLAEG
                     KLQPLLRAPIGSVLKPTRRRPVAIVPRAQLTPQLAEGRIVLDLGDERFWLLPRNLADR
                     TLLFTMRHGISRVESKTHRVGRRLANQLDPERGIPRADAVGAALARMVGVVGQQLDFL
                     HLTNYLDPRTFLHFISRSPNTRQLFERVVAALVQGPTAPPEPVWEPALESSDFGWLTG
                     VEKTVEAQELAKAFGVDVPIAKRLIKDPLYCYPGGNSFFDLYVDVIDGLHGLGEANRG
                     KVACLYTHSSTLRALMIYLDPRPFHEAFNEFSDYKESQDNVVLLTFEKGRLSGYSTAV
                     GLSERERASRDSWVAVERVRKDRVALKPGQIKRIVALVSGGDFAGAGAALKELRVAGN
                     RFGLDVHFVRHGFLGLANNWIETVTEEDTRGMSSHASSPIGSSRFEDFKEEQVQQAAM
                     RHLAPFLKDAALVVLGGDGSLRGARAIYEGFGIQVAGIPGSIDNNIEGTTSLGYHSAI
                     ALADKSIESLKATSAAMGSVFFVEVMGAGSGHLALGCAYQARAEGLLVNEYPDPDAYI
                     DEVILGTLKRTLGVPNKSHLFVVAERTPHRHHREGGVHGLVDYVAGVIAEWPQRHARP
                     GQYPLNLATKATILGHTLRGAPPTPEDKAIAQHLAYETVRRLVERPEDVVGCMLAYRE
                     RGTIEAIPLHAVAAKQFDWDAFARMHGSELLENRS"
     gene            complement(35039..36367)
                     /locus_tag="VLD60_06190"
     CDS             complement(35039..36367)
                     /locus_tag="VLD60_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44581.1"
                     /translation="MSEHELEKLLGGFAAGTLTAEERNMLYTAALHDQQLFNGLADEQ
                     ALKELLSDPAVRRRLLQALNQTSPSGAGGSLSWLDWFRRPASLAFAGSLAVAVFAVVL
                     GTKIYQDSLKQAAQPVVTEHMTPAAPPAPAPSTSQPTSPQVAEPESKAKDNLLADKMA
                     KRERAAPPALEQQQTPDAARDSAPLRSEQDAARKQGEPPVAALGKTTAEPTAATDQKP
                     SASSASTVSARTLFYGEPVARPDGPVTAQEKEQPMKPLAESAPQGNRPEQKLDRFAAA
                     GKAAEAVVQLKALGLRYSFVIRGADGQDREVDAATASRSGGHSRLTVEANQPAYLQIW
                     KTEGSSTPQLLFPEKEFGQISIKLAAGQRQQVPLPPEIGTAALTVRLSRVPIGPITRQ
                     EAAMLGRLSPNQFQESIGGAPNSQEQATYVVTQDSSSTAQLSTEISLSLP"
     gene            complement(36455..36952)
                     /locus_tag="VLD60_06195"
     CDS             complement(36455..36952)
                     /locus_tag="VLD60_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB44582.1"
                     /translation="MDRDQILASLRERILAFATLRVSREQAEDLTQEVLVLLHEKYAL
                     VTELTELVPLAFQVLRFKMLDAHRKSLRRGEYNQQSVEDLPLADPGDDPATRLDQKQR
                     VDRLLAAIAQLGERCRELFKWKLEGKSFPEIQELMGQTSLNTIYTWDFRCRKQLLSLM
                     GGSWE"
     gene            complement(36968..37939)
                     /locus_tag="VLD60_06200"
     CDS             complement(36968..37939)
                     /locus_tag="VLD60_06200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB44583.1"
                     /translation="MTGKTLIDCHVHLAALPDGDNGCYISPRLLKSPLFRFLLRKHEL
                     SPAHPRESNQKYLDHLITELRASRHVHKAVLLGMDGFYDQTGLLNRQHTEFLISNDYV
                     LKTAQAYPDIFLAGPSINPQREDAVEEVHRCAEAGAVLVKVLPNAQHFDPADSKYRPF
                     YRALAQRKLPFLSHVGFEFSLIGKDQSVGDPDRLRTALDEGVTVIAAHACSYGLMLYE
                     KFIPTFHDFAKRYHNFYADISALTLPNRMRMLLHLRRHPEFQDRLIFGTDYPLSVFHL
                     AAWGRVGIRALAKMIRTTNRFDRQVAVCRGLGLGFRSFNDIVAQPGL"
     gene            complement(37969..41394)
                     /locus_tag="VLD60_06205"
     CDS             complement(37969..41394)
                     /locus_tag="VLD60_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HSB44584.1"
                     /translation="MTKHSRYPLRGLLIAQFCGAFNDNAWKLMVALLGIRQLASTMAP
                     GPDFETASQTQTTLAFVVFTLPLALVSIVAGVLADRISKRTVIISMKSVEVLLMAAAT
                     AALWINPAGGILPLIVLGGMGVHSALFSPAKYGILAELLPHERLAAGNGQLEMWTFLA
                     ILTGTAAPGILLSLTGDSTWLAPLLLVGFSLVGFRSAWTIPRVAPARAEGGVVNTLQG
                     AWSALAADRMLRLAITGAVFFWTIASLFAQNILIYAKAVLGVSDWQSGLPLMMLSIGI
                     GLGAILVGRLSQSRVEYGLIPLGAAGVAIILTSLGLFAPGFAATMALMVVLGTSSALI
                     FVPLNALIQWRAPADRRGSVIAFENTCVFTGILLGSLGAGALAQSGLATIGIFLATAA
                     FTILGTAWALWLLPEALLRVVLVILTNSLYRLRIFGHEHVPTTGGALLVPNHVSFIDG
                     FLLIASLDRPVRFVVDAEYAEQRLFKPFMKMLGVIPISQHGGLRVVLRALRDAGAAID
                     RGEIVCIFPEGQITRTGTLLPFRRGFERIMKDKTAPIVPVHLDRVWGSIFSFNRGRFL
                     WKLPDRIPYPVTVSFGSPLPSDTSADELRSVISSLGEAAWQRRRPDRRPLHREFISAM
                     RRRPFRFVMADHIRPRVSSLQALIGSIALARALRSYWDGQNNVGILLPPSVAAALVNV
                     AAPLCGKTSVNLNFTVGKSGLEAAVRLAELRTVVTSRAFIEKAKLELPDGPTIIWLED
                     VAKTIGRAQKLLAALFALGAPGRLIELACGQRTVLKIDDLATIIFSSGSTGEPKGVML
                     SHFSVDSNAQAATQVLHLYQKERVLGILPFFHSFGYLVFWFVMFNDAAMVFHPSALDV
                     AGIGEVIRRHRITFLVTTPTFLQLYARRCTPEQFGSLRVILTGAEKLPARLAQTVEDK
                     FGIEPIEGYGVTECAPVIAVNSPDFRAAGFYQPASRRGTVGQPLPGVSVKIVEPDSFV
                     PLPPGTPGMLLVRGPNVMKGYLRREDLTAQVMRDGWYITGDIAILDDDGFLTITDRLS
                     RFSKIGGEMVPHGKVEEALQLAAGLETQAFAVTGIPDERKGEQLAVLHILEEARIPEI
                     LAKLAAEGLPNLFVPPRSNFVRVEALPVLGTGKMDLRGLKRIAMERLAGERKG"
     gene            complement(41429..43168)
                     /locus_tag="VLD60_06210"
     CDS             complement(41429..43168)
                     /locus_tag="VLD60_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB44585.1"
                     /translation="MSVTWRNSLSHIATGCSVSENFASSLALLLVGSFSFFYNLDIAL
                     FEGSEGLYAHISREMQLSGKWFQLTYHDEPYVNKPPLFFWILALFTRWFGENEIALRL
                     PGALFSLGTMMLTYFLGKSLFSRTAGFWAALVVATSHLMLWYGRRALFDSMQTFFITL
                     ALFAWAKAHLQNAGRWWYPVVGVGMAFAVMTKGLHGAALPLVVIIGFLALTRDSSPLK
                     NRSVHTTVLLLFALIAAYASLLSEDLVLHFNPFKGLKSAFAFSSHTATPGHPMYWYLL
                     IIWFDFFPWIVLLPSSLIWFMSRHPLRLERSELFVLLWFLGLFTALSLSTLKRESYLM
                     PLIPPLGLMIGRYCQSLYCSPASKGLATMLLRAMLGLLAIAFVIAMFYGPGLLQKRWE
                     TSLSVFPPAFVMIVLSLATLLAYAVIRSTIKLALGTVGALAVSFSIAVVGLILPGIDD
                     ATSARRASDTIKSFPHNPSEPLHLFSPRWPDNEDVVYYLNLEPALPRLASEDMLMSKV
                     REAGHVLAVMDNTSFATLKRKPDLALELVREFRQRRNKNLYILKITLAAAADDREPGL
                     SMLDVALPSDGLG"
     gene            complement(43347..45176)
                     /locus_tag="VLD60_06215"
     CDS             complement(43347..45176)
                     /locus_tag="VLD60_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HSB44586.1"
                     /translation="MAPFSRTAQLQRLIARLGRLIDQGTSASSRFTKWRLAIFIAGLL
                     CVVTLYKSGWYSTGNLVLAGFVTLFLIVAAYHTRLEQRIHRLRMWTGIKSAHLARVRL
                     DWPNIPTRPSETQASHLYAQDLDLAGSHSLMHLLDSTVSDQGRLRLSGWLMTQPPIQP
                     VWEARQRLVKELTPRSLFRDRLELEAHLAGEQEINGRRLAAVLDHAVGFPRLNLILTI
                     QSLLALTTIVLGLGTLLNWLPGYWMFSFAAYALTYLQTDQGEELLEHAIGLHHEMERL
                     GKVLGYLERHAKRRGSALATACAPLLVPGARPSAHVAQAARTLHAISIKAHPLVHLAV
                     NVLCPWDLWWTKRLQQIQQAIQKQMPTWLDRLAEVEAACALGTFAYLHPSYVWPVPGQ
                     HMTLIATQLGHPLIQQDTRVANDISLSKPGTIHLITGSNMSGKSTFLRTIGINICLAQ
                     AGAPVCATTFQWGWSRLACCIRVNDSLDAGMSFFYAEVKRLKSILDATEDRAASPVFF
                     LIDEIFKGTNNRERLIGSRAYIKALSTGNGLGLVSTHDLELTDLEKEIDSLTNAHFQE
                     TVSGGALEFDYKLRPGPCPTTNALRIMQLEGLPVSSEKTAGQS"
BASE COUNT         9372 a        12586 c        13954 g         9682 t
ORIGIN      
        1 atgaaaggac atgtcatgaa gatcctcgga ttgtgcctcc tcttgatcct gcccgctacg
       61 ttgtgttggg ctgattctga atcgcagaaa cccgaggaac agaaatcaga ggaccagaaa
      121 cccgaggagc aggagcccac cttgtacgat cactatcatg ccatgaagca gctgcaagag
      181 gcggcggcaa gtcgtaccat tgatgaacaa gctcgattcc agcccttgat tcagagagag
      241 cagcggctgg cttgtgaacg gctcagaaag gatcaccgta agcgagtcca gaaggaggaa
      301 taccgtcgcc agggagggga cgaattcctg gttttctcct gggaactcga acaatggtgt
      361 gggacgctcc gctaagcagc gcttggttaa gaagctttct gcaagaaatc tttcagctcg
      421 atccggcact cgaaatcata ttcttattcc gggctcgagt ttgggcatat gtttgtcgca
      481 ggtgtactta ttgctgggca gaggcgaggt gctggaattg caatcccgtg gctcatgctc
      541 acgatcatcc ctcaaagata gcgcctttac acacgtaccg gcggtcgatg ggctgcgtgg
      601 tatcgccatt ctcctggtcc tttggtatca cgccccattc ctctttcgag atcttcctga
      661 gtttggtatt aaccattccc cctggcaggc tctcggtgtt gctggaacga tgagtcttgg
      721 tggctggata ggcgtggatc ttttcttcgt cgtcagcggg tttctcatca catcgattct
      781 gcttcgcttg cgagattggg gaggctcctc atgggtgttt tggggaagac ggggagtgcg
      841 gatccttccc cttgtcatgt tgtatctcct gatcctattt gcccttgcat ggctcggcga
      901 tccactgaag atgttgcctc actttgaggg gtgggcgtgg tatgccctct atctcggcaa
      961 tatccatatc accttatacg gatggcaacc gctggccgtt atgatcctgt ggagtttggc
     1021 gatagaggag cagttctacc ttgtctggcc gttggtcgtc cgagcgtgca gtagacagcg
     1081 gttgctgtgg tgtggcgctg gcatgattgc tgtggcaccc gtggtaaggg ccatcatgct
     1141 ctcgacatct gattaccctg ctacatacgt cttcaccctc tgccgccttg atgcactcgc
     1201 tgctggctcg gtggtggcgg ttatatttgg gagtgacacg tggaagtccg aagccgcctg
     1261 tctctgcaaa agactcgcac ccttcgcgtt agtcttgata ctcatgacat tccttgttcc
     1321 atttagtcca tccctgcctg agaccaggcc atggttcttc agcgtcttcg ggtactcatg
     1381 gttggccgtg agctatgcta tcctcctcgg cgctagcctc aacgcggatg gaatttttgg
     1441 tgcaattttg aaatcaccgg tgctcacgat cctggggagg cgctgttacg gactctatct
     1501 atggcatgtt ctcatcgcag gtttcgtcat cgctgggttg caactattcc aactcggatt
     1561 ttatgtacat atggtcctct ggctagggac tctcctaatc gtcgcgagcg ggtcctggcg
     1621 gtttttcgaa gagcctatcc tccggcttaa acaatttctt ccatacgggg ataacgaatc
     1681 agaacgtttg gagtcgaaac tagtggcctg aatgaatgcc tcgacgccac gtgccgtcgg
     1741 atcatgggca tcgatcccta tgaggtttct tatttagaaa gggccggtaa ctggccgggg
     1801 ccaattgcca agggagcgat ctagcagcga ggagagtcta tagccgtagt acggacaaac
     1861 ttcaatctga ttgttcaggt ccaagaaagc ttggcaggtc gtttgcctga tgagctagtc
     1921 cggtcgcaac ctcagatcct gctcagttgg cgtctctcag gatactctac atgatttaag
     1981 ccatccccaa tatgagcgct aagtgtcgaa caatgaaggg agctaactcg ttgggagatc
     2041 aatgagcctg ctcgccgttg ttgcccgcca gacctgctac acttgaattg gacttgtaca
     2101 gggaggggta tgaagaaggg gctgtggggt ctgacgatac tcgcgtggtc ttgctctgcg
     2161 gccttggttg gagtcatggc ggaagagtca caggcagctc aaaagaaggc caaagagcag
     2221 gcctctgtcg tctcggccaa gtctccggcg ccagtgcctg agctctctca aaagccttcc
     2281 aatgccaacg gaaagaaggc cgccgcatca gtcgtgctag aaaggcagtc tgaagttctc
     2341 cagcaacagg ccaaacagac gatgtcagag gtcaagccga cgactcagaa gggccatcgg
     2401 cggatcaacg tccataagaa gcatgcgccc aaggccgtgg tacagccaag acaggacctt
     2461 gcgcatcacg gcctgcttga agattctcaa cggtacgatc ctcgtctcaa tcaccgcacg
     2521 gccggcgtcc agaatccgca gacgcctgat ttgacctacg atcattttca ggagctggat
     2581 cgtaatcagg acgggaagat cgatccggtt gaacgggcct tcgcccgtct ggatatggat
     2641 cgagacctgc acgaccgcca cctgcagtag ctgctccttc acctcaacag cttcagttcg
     2701 tttcattcct caaaagttcc ctcactttcg tagaactgat acgagttgaa gtgctgcggc
     2761 tgcccgcctg ccgatcggat tatcctgtca atgtaaggag accgggctct gaccgactga
     2821 gggtgtagca accgacattg acagtgaaga aaggagcccc catgatacgc gaacagacca
     2881 tgccgaatga catcacagct gcccaaccag ccagcggatc aagcctcggg aaaattgcca
     2941 ttgcagtcat gattgtttgc gggatcggag cttttgtgta cttcgacctc ggaagatttc
     3001 tttcgcttga gacgctgaaa gagaaccgtg atcatctcct ggcctttacc gaagccaatt
     3061 atgctgcttc ggtcgtgctc ttcatcctcg cctatattgc ggtgacaggt ttgtcgctgc
     3121 caggtgcagt gattcttacg ctcgccggcg ggttcttgtt cggcagtgtg ttcggcaccc
     3181 tgttcgtgaa tctcggagcg acgacgggag ctacgctcgc cttcctcgcc gcccgctatc
     3241 tgttgcggga ttgggttgaa cagaagttcg gaaagtggct ggggccgctg cagcaggggt
     3301 ttacggctaa tgccttcagc tacctcatga cgctccggct gattccactc ttcccgtttt
     3361 tcgtcgtcaa tctcgtgtcc ggcctgacac ggatgaacat cgggacttat gtggctgcca
     3421 cggcgctcgg catcatcccg ggatcattcg tctatgccta tgccggccgc caattaggaa
     3481 cgatcaactc gttgaaagaa atcgcctccc ccaatgtcat tgccgcgttc gtgctgctcg
     3541 gactcctggc gttggcgccg atcgtctaca agaaattcac ggcgaaacag gcgtgaccgt
     3601 aaaccgtgtg aaacgtgagg catgaggcat aaaagtacag gaaggttgcg atatgattac
     3661 tcatacatca gagaaggtgc cgccaatgcg tcagcatgaa gaaggtctcg tcctgccgaa
     3721 cgatgagcac aatcgcattc ttgctgaaaa cgtccatccg tccgactggg tcaaccccga
     3781 gccgaccggc cgctataaca ttgtggtcct gggggctggg acggcgggat tgatcacggc
     3841 tgtcatcgcg gcgagtctgg gggccaaagt cgcattggtc gaaaaacatt tgatgggcgg
     3901 cgattgcttg aacgtcggat gcgtgccctc aaaaggtgtc attcgcgccg cccgggcctg
     3961 ggccgatttg cggaatgcgg cggagttcgg tctgcatatt cctcccggag tgaagtacga
     4021 cttcggggcg gcgatggcgc gtatgaggaa gctgcgtgcg cggatcagtc acaacgactc
     4081 ggcccatcgc tatacgaagc tgggcgtaga cgtgtacatc ggcagcggac gctttactgc
     4141 cgccgatacc atccaggttg aagggtctgc cgggagtcgg acgttgacgt tcgcgaaggc
     4201 ggcggtttgc accggtgcac gagcatcgtc acctccgacg ccggggttga aggaggcggg
     4261 ctatctcacc aacgaaacgg tgttctcgct gacagagctg ccgcaacgcc ttggcgtcat
     4321 cggcgcaggc cccatcggtt gtgagctggc gcaggcgttt gctcgcttcg gtagtcaggt
     4381 ttacttgatt gaagctttgc acggcatcat gccgaacgaa gatcgtgacg cggcggatat
     4441 cgtcgaacag cagatggtgc gagatggtgt gaagttactc tgttgcggaa aggatctgaa
     4501 ggtccagaag gccgaagacg gcaagcggct tacggtcgat tcgcacggac agcagtacga
     4561 cgtgacggtc gacgagattt tagtcggcgt cgggcgcacc ccgaatgtgg acggactggg
     4621 attggaagcg gccggcgtcg agtacgacaa gaacggcgtg atggtcaatg ccaggctcca
     4681 gacgacgaat cccaggatct ttgctgcggg cgacatttgc tcaaggcaca aatttacgca
     4741 tgcagccgac gccatggcac agatcgtcat tcagaacgcc ctgtttccgc atcccttcgg
     4801 gtttgggtac gccagcgtgg aatcattaac catgccctgg tgcaccttta cggaaccgga
     4861 ggtcgcgcat gtcgggatgt acgagaagga tgcgaaggag aaaggtctcg aggtcgaaac
     4921 ctacacctac aaactcgatg aggtcgatcg cgcgatcctt gacggggaag acgcggggtt
     4981 tgcgagaatt cacatccaga agggcaccga taagatcgtg ggcgcgacga tcgttgccgc
     5041 ccatgccggc gaaatgatca gcgagttttc cgtgctgatg aaagccggtg ccggagccaa
     5101 aacgcttgcc ggaacgatcc atccttatcc aacgcaggct gaagtcaata aaaaggtcgt
     5161 gaatctgtgg cgcaaggcgc atttcacgcc acgaaccaag agtttgctaa ccaagctatt
     5221 tgcgtggctg cgtcggtagc gattaacaag acatgacagg ggcgcgatcg gtacatagag
     5281 catggctatt cggcctaggg ctgttgatcg ggccgatatg cctctgggcg caggatccgc
     5341 cgctactcga agtagcgaag ttttcagccg gtcaggtagg tacgggtctg ccggagggtt
     5401 ggaagcctct gaccttcaag aaagtgccga agcaaacggc ctacgagttg gtgaaggatg
     5461 gagacgtggt cgtcgtcaaa gccgtcagcc aagcctccgc atccggtctc accaaagaag
     5521 tcaagatcga tccgaaagag tttccgatca tacagtggcg gtggaaagtc gagaatcttc
     5581 tgaagcacag cgacgtggct cgcagggagg gggacgatta tcccgcccgg ctctatatca
     5641 ccttcgaata cgatcccgag aaggtcagtt tcgggaagaa gctcaagttc aaagccggtc
     5701 aggctatctt cggggatatt ccgatcggtg ccttgaatta catttgggag acaaacgcgc
     5761 cggtgggcgc catcctcgac aatgcctata ccgattttgc aaagatggtc gtggtggaga
     5821 gcgggccgca aaaggtaggc ctatgggtgg acgaagcgcg caatatttat gaggattaca
     5881 agagagcctt cggggaagat ccgccgatga tcaatggcgt ggcgatcatg agcgatacgg
     5941 acaataccaa ggaaacagcc attgcctact atggcgatat ccagtttgtt cgcgcgaaaa
     6001 aatgacagag gcagggaaag tagaaaaaga agagaccgac ctgttcaggg ccggcctctt
     6061 catggacacc aacgcgccca gggtgggtgc gcggattcaa aggggggtta ctgtatcgaa
     6121 ttcgcgaagc cgccccgcgt aatctctgcg cgaacggtgt ccccgacctt cttcttgttg
     6181 atgtgcttag tgccctggcc gacgtacagc ttcacctgat tgccctcgta atcttcgacc
     6241 aggtaggcat cgttctcgat ctgcttgacg gtgcccccga ccgtcctcca ggccgggcct
     6301 ggtttctcgt catggcgacc ggctttaggt gcatctggtg cttgggggat ggatcccatc
     6361 gatgatgcag ctgccgccgc agacgaatgg gacgaagact tctttgagtt cttggccact
     6421 gccggcccga cggcaagagc tgtcaccgcc aacgctgcca cgatgaccgg gagaatcttt
     6481 ttctttttgg cctttttcat gagtcaacct ccttaactga cgtgaaaatg cttgttcagg
     6541 ccggaataat cagcaaggcg tgtgcccgca ggccatgcgt tgataatcct taattgtcag
     6601 gaggttatga ccagttgacg aagagcaagc tcaaaagccc agagaaagcg gaaaaaaaac
     6661 ggcgataccc tgtgcaaatg tgatcacaat ggcatcagat ggcatgatca aaccgaaggc
     6721 ttctttggag ccggaaaacc ttttcctacg gagcgaatgg gttcaattat gaatcgctcg
     6781 gtttctctgg gcctggcgtt cattctggct ggctgcgcct cggtgccgac ttcatttcac
     6841 ccacgagatc cgatctcccc gcctgggttt tcacatcatg cgtttgatgc cgtcgtacga
     6901 tctgctgtca gtaacgggca ggtgaactac cgtgctatcc agagtgacct gcggtttagg
     6961 cagtatctcg atcagttgga ccgcatcgat cccaacggct tctctcgcga gcatcgattg
     7021 gcattttgga tcaatgccta caatgcgttc gccatcaaag gcattctcga cggcgaatcg
     7081 ccggctccct atgtaggctg gtaccgctat ttcaaggcac gggactacgc cgtcggggga
     7141 gcacgagtga atctctatga cctggaacat gggatcttac gcaagcaatt caatgagccg
     7201 ctggtgcact tcgccattgt ttgtgcctcg gcctcttgcc ctaagttgca gccatgggcc
     7261 tatgacggtg cgacgctcga ccgacaactc gacgaagcgg tgcggggatt catcaacgat
     7321 tcggctcgca atcgattcga tcggcaacag aaagtggcct cgctgtcgat gatctttaaa
     7381 tggttcgagt cggatttcgc cggggctgca gggtcagtgc tcgcctttat cgccccctac
     7441 gtcaacgatc ccgaactggc aagagaactg tcacagccag gctaccgcat cgagtatctc
     7501 gagtatgact ggagcctgaa cggtcttcca cccgaggagt cggcgtatgc tcgtacgtct
     7561 tgatgaaaaa gtcccgattg cgcggctatt gacctttctc gaacatggtg agcgaatggc
     7621 gaacgagtgc gcgaaggccc aggcagggtt tgccctcgat gcgggatcgc gccggttcct
     7681 catcacccag gcgcggcaag agtccgcaca cgcgatggta tttcaaggag caattgcctg
     7741 gctggcgccg aggcatcttg gcgatgcgcc gtttctccct gcgctggaag agtatcgctc
     7801 gatcctcaac gaggcgttaa tccgacagga tctgtgtgaa acgtttctgg ctgagcaagt
     7861 cattcttgaa ggattaggcg aagccattct gacgcgcatc gaagaggggt tggtcaagcg
     7921 tgcggcaccg ttcggtcggc tccggcgcat cctgttgcag caggaagagg cccatcatgg
     7981 attcggacgc cgcatgctgg aacgggcgac ggccgaaggc cggaccgatg cggagacgct
     8041 gcgccgtcgt gcgcaagact acctcgcctt gacggaccag atggtgctga ccttgagcga
     8101 tctgttcgaa tcgattgccg aagatcccac ggcgtgggcg aaggatgttc ggaagtttct
     8161 gcctgagtgg ttgcatcaag aggagcagtc atctttcaac aatcagtcat cagccctagg
     8221 atgttccgaa aagctgatcg ctgaacgctg atggctgtga gctgacccat gatttccgtc
     8281 gtgatccctg cttacaacga agagaaagcg ctgcctgaga cactccgcgc gctctttcgg
     8341 cagcctggcg actacgaagt catcgccgtc gacggcggga gcacggatcg taccctcgcc
     8401 gtcctttccg agtttggttt tgaacgtccc tcgccttctt cccctcatcc ctcacggcag
     8461 ttgctaacgg cacgaaaagg ccgtgcgtca cagatgaacg ccggggccct gcaggcgcgc
     8521 ggcgagtggc tgctttttct gcatgccgat acgaccttgc cgcccgaggc cattcagcga
     8581 ctgaacgaga tggacttgga cctcacgatt caagccggag gattcacgca tcaattttcc
     8641 ggcaacgact ggcgactcaa gatgatttcg tttcttgaca attttcgttg tacgcgcagc
     8701 cgaatcatct acggcgatca agcgctattc gttcgccgcg cattgttcaa acggctcgga
     8761 gggtttcccg atcagccgat cctcgaagac gtggcgttct gtgagcggtt gatcgcagga
     8821 accactccag tactcctctc gccgcccgtg atcacggatg cccggaaatt cttgaaaatg
     8881 ggcgtttggc gtagctttct ccgcgtgctc cttatcatcc tccacgttga attccgtttg
     8941 ccggtgcttc ctcgcagatt ctttcaggat gtccgatagg accctgccaa aatcacacaa
     9001 gcgcaggatc gtgcgtccgg ccgtatcgac tgcgagaaac acgatcgcca aggcaagcgt
     9061 caccactagc gttcgaaccg atgcagaaga gaagggggct cacgagatcg atcgtgagcc
     9121 ccccttctcg ttcgaaggag tgaacctatc gaccagggaa cagcggggcg ctagcagggg
     9181 tgttgcgaat gcagtgagat tgctacaatg cgcacaatga cgaaccaggt gaagcggact
     9241 ttcctgctag agcgactgcc tgcacggcac ttgggcatgc tcagacaaat cggccggctt
     9301 gccgacgagc aggacctgcc tgtctatctg gtcggaggag tggtccgcga tctcctgttg
     9361 aagcggacga actgggatct tgatctcacg gtagaaggcg acggtattgc cttcgcgcgt
     9421 ctcgtcgcag accgatatcg cgcaggattg gcgacctttg agcggtttgg gacggccagg
     9481 ctcgtgatgc cgaacgggat cagggtggat atcgcctcaa cgcgccgcga gtcgtacatg
     9541 cgaccggcgg ctttgccgaa cgtcgaaccg gcgactctac ttgaagatct ccgtcgtcga
     9601 gatttcacga ttaatgccat ggcgattcag ctgaattctg ccacgttcgg gcggttacac
     9661 gatccctacg ggggacagcg ggatctcagg gcacggacca ttcgcgtgct gcacgaaggt
     9721 agtttcgtcg acgatccgac gcgcatgttt cgcgccattc ggtttacgca gcgcttcgga
     9781 ttccgtctgg agtctcaaac ggcgcagctg ctaaaagcag gagcagccac ggacctgatt
     9841 gcgcaattat ccgggccacg gttatgcagc gaaatcttgc tgctcgctgg ggaggtcttc
     9901 cctcagcggt ctttcgactg gctgagacgg ttgaagctgt tacgattctt gcaccctgac
     9961 ttgcgatata caggaccggc gagacgaatc gtgcagtctc ttccaagtgc gttagcctgg
    10021 tggcggacgc gctgcgacgg caattcagtc gaccgcccgc tcgtgtatct gatggcattg
    10081 cttggtggag cggggcagcc cattatcgaa gcggtggtga cgcggctcat gttgtccaac
    10141 gagcagggaa ggaaggttcg cgccggtggg cagagactga agagcgtcgt gtgcggactt
    10201 ggtgaaacgc gacggctcag gccatcagaa gtccatcatc tgttgaacgg cttgcccgat
    10261 gaagcgttgg tgctctgctt ggcaatggta aagaagcgga ctgcgatgct tgcccgggtg
    10321 aaacggcgaa tacgtgatta tgtccagaga ttgcgcgcga ccaaacccgc gttaggcgga
    10381 gatgacctat tgcacatggg agtcaagcca ggcccagcgg tcggcaccat actcgccctg
    10441 ttgcttgagg cgaaactcga cggtgtggca aagggaaagg ctggcgagcg agcctttgta
    10501 cgggctcgac tcgccggtag aggttaggtt gagactgttg tcagacccag agaacactgg
    10561 gaacacgggt tcttggcgaa tccgaaaggg gacagacgac accccccgag ggtgttggaa
    10621 agctcgtcca gcaggccgca gcaagcgaaa ggacgaggag gtacataccg agcttcgctt
    10681 gagccgctcg cttcgatcac atgggagcgg atagttactt tgcctcaagt cggctttagt
    10741 atacgttgag gccttgagtg atgcgagaat gccgctggag gacttttgca gcaccctcaa
    10801 ttcaacggaa tgatgcttgc ggcgacttcg ttcggcagca agagggtcac tgtcgcccga
    10861 ttctcttcgt ccggctggat caaggcaaaa agcggaatcg gctgcggcac gctgttcggc
    10921 ggaaaggcca gcaaggccgg gaaatcaata aggggcgaaa cggtcggttg tgtcgccagg
    10981 cgaagcctga aggccatcaa gacgtcgcgc aatcggttgg cctggtcttc ctggctgagc
    11041 gttttgacag gggcaatgcc cacatcccag agtggcttgg ttaccgtcac aggaaacgaa
    11101 aatcccagtt gcttggccgt cggtgtgacg tcgatcaagg ctcccgtttc gagtgcttgc
    11161 ttaaagggta tcttggtcgg cacattccca gtctgctcca ctccctcggt gcttggttca
    11221 gcggttgagc cttccggttc tgtgactgat tcaggaggag ccagatcact cgttggggca
    11281 acttcctctg agatcttcga ggcatctacc ggtatttcgg agggtgccgg taagactttg
    11341 ataatgccct catagacagt tcgagcccga gccgaccatt tagggttgag gggctttccg
    11401 gcaaacgcgg cttccgcggc attcttctcg tcgtgggaga gtgctcgagg ggtctgattg
    11461 ttatccatga gaatggagat aagtagggag gcgcgcaagt caagggctga aagcgggcta
    11521 gtccggtagc ggtttccctt tcgtttcagg cgcaaaaagc aagactaaca gacccaggag
    11581 gtagatcgat gccacagtgc tggcggcgcg accgaacgtg ccaagtgtcg tcacgagcca
    11641 gccggtcaca aatggtgcga ctgatgcgag cacgcgtccg gcattgaagc agaagccggc
    11701 accggtcgtc cgcaggacgg tcggatatag ttctggcaga tagatgggga acccgctgaa
    11761 aattccgttg ttaaagaagc cgagaattgg cagcagcatc aacacgccgg cataggtcga
    11821 gggtagcagg taggtcaccg gcaacatgat gaaactgccg aggcacatga acgcaaagac
    11881 tggtctcctt ccaaatacgt cagccagcgg accgaatccg aggtagccga aaatggcgcc
    11941 ggcattcagt gccatgatgg catagctgac gtacttgctg agtgtggcgg aatcctgtcc
    12001 gttcagatcc ggtagctcgc ggataagtgt cggggcccaa ttcgtcgaac cccaaaggcc
    12061 gaacacggcg acaaacgcta acgctgaccc caccagtgtt cctcgccgta agttgccctc
    12121 aaagatcgcc gtgagaggga ggacctgttg ttcatgcgca tgtgtccaac gttccggttc
    12181 cttgacccac caccggacga gaagcgccac gaatgccgga aggatgccga tgaggaagag
    12241 gcctcgccac ccgtacgcat cttggagcgt caggttgaat gtcgcggcaa ggaagaaccc
    12301 gacggcccag gccgattgga ggatccctgc agcttttgct cgcttcgctt ccggccaggt
    12361 ttccgccacg atcgctgcgc cggctgccca ttcgccgccg atgccgagtg ccgtgagaaa
    12421 ccggtaaatg gcgagatgcc accagtcttg cgacagggcc gccgcgccgg taaacacggc
    12481 atagatgaga atcgtggcga tcaggatctt cgtccggccg aaccggtcgg caaggatgcc
    12541 aaaggtaatc ccgccgatcg cccagccgat caggaaaatc gaaaagatga tcccgccgta
    12601 ccagccgatc tcctcgggca aaaccgtggc accagcggag gtttgaagca gttcacgtag
    12661 agcggggtgc aacacgatcg catagatcgt cgcgtccatg gcatcgaaca cccagccgag
    12721 ccaggcgacg aacagcacaa gccactggta acgcgtgaca cctgatcgcc aggactgacc
    12781 actcacgaag gcaccccgca ccgaggaaac tgaatcactt gagtcctgag tcgtgagtgc
    12841 tgggctgcga gtgaaggcat gggttgcgac aggctagagt ccttctaggc aggagtcaag
    12901 gtgaagcgtc aagaagggcc gtgctatagt cgcggccatg gccagggtcg atttttacag
    12961 ggtcctcgga gtctcgcgag aatcgtctga cgagacgatc aagaaagcct accggaagct
    13021 tgtcttcgag caccatcccg atcgcaatcc cgatagcaag gaggcggaat ccaggatccg
    13081 cgaaatcaat gcggcctacg agatcattgg tgatccggag tcgcggcgca actacgatcg
    13141 gctcaattgg ggcgatgagt tcagggaaga cgttgccgat ctctccgtca tcctcgacga
    13201 gatggagaag aagctgttcg acgaagggcg aaaagaactc ttcgccgttc ttgtcaaaga
    13261 ggtcgcacgg atcaaggctg agctcgccct cattcgggag cggacggtgg cggatcaagg
    13321 gtacgattca tttaaagaag atctcattca tgcgcgcgcg gcggaggtca tgcacgagtt
    13381 cgtaaccgat gagatggaaa gacgcaaaca gcggttggtc gacgtggcga cgcagatgat
    13441 ggtatcccaa ggtgtcgtgg ggaaaaacga cgaagggggt atccgttccc ttcgcggccg
    13501 gttagacgag cagttttgca aaggtcgcct gcacgggttt gcctcggcgc tggagctatt
    13561 ttacgaacgg cggtaataga aaacagttct gggttctgag ttcagagttt cccggcccgt
    13621 cttcggaaac tcagcactca gaacttacaa ctcagaactc aatcttttga gagcagtcct
    13681 gccacaaact tacccgatct catgacgccg acgattcggc cacggtcgcg gagcagttcg
    13741 atcttccgca gcggattgcc atcgatcaaa atcaggtcgg cttccttgcc tcgttcgatc
    13801 gtgcctacga tgtcttgaat cccgatcaat cgtgcggcgg acgacgtgga agccaggata
    13861 gcctccatcg gactcatgcc gaacgtgacc atccgttcga gttcctgcgc attgtcgccg
    13921 tgataattga acggcgtgcc ggcgtccgtg cccatcgcga tcaagagacc gcggcgatgg
    13981 gcctgtttga agctggcctg atggcgcttg gtcatggctt tggccttgtc gagcgcgctg
    14041 tcgggaatgc cgcagcctcg gcggcaggcc gcggtcgtag cgagagcgga gagggttggc
    14101 accatgtaga ccgcctgttc gttcatcaag gcagcggatt cttcgtccat gagcgtggca
    14161 tgttcgatcg aatgggcgcc cgcgcgaact gcgttcttca tcccggacgc tccgtgcgca
    14221 tgggccgcca ccttcagccc cgctcgtttg gcttcggcga cggcggcctg cagttcgtcg
    14281 agcgtcagct gggcttggtc cggagaggtg ccgggcgtga gcacgccgcc tgaggcaata
    14341 actttgatga cctgcgcgcc ggcggcgatc tgggcttgaa cgaccttgcg gacttgctcc
    14401 acaccttcga cttcttgccc gatgaatctg gcgtgcccgc cgatcatgca aatggcgagt
    14461 ccggctccga caattcgggg ccccgcggtg agtcctttgt cgatcgaccg tttcagcgca
    14521 aagatggaat gatctcggga gccgacatcg cggacggtgg tgacgcctgc ttccagggtt
    14581 tgccgcgcca tctgactcga tttgagcaat gtttcggtcg gagtttcctg ctcgatggcc
    14641 tgaacgacat ccggctccgc gccgagacag agatggacat ggcaatcgat gagacccggc
    14701 agcagggtca gtccgcggcc gtcgatgcgg gtggcgcctc gcgggatcga aacggcgcgg
    14761 ctgggaccgg ctgcgtggat tttgccgctg cgaacgatga cagtggcccg ttcgatggct
    14821 tgcccgttcc cgtcgatcag tcgggtgttg tggatggcga tggccacggc cctattcccc
    14881 gatcggaaat gtgatgacga tgccgcccat gacatcgtcg agcttgaagt cccggcgagg
    14941 gctgttcgga tgttggttgt ggcaatcgac gcaggcctgc gacacggcgc ggtctgcata
    15001 gatcgcttga aagaggcgga gctcgccttt tctgatgaga ccgtaatgag gtcgcgaggg
    15061 gtctttttgg acggctgcta aaccgacccg ttcaagatcg ctgttggccc cgttccagac
    15121 atagatcggc gtcaagctgg ccagccgaaa cgagaaattg aggtctttga gcgcaacgag
    15181 ccggccggac tccaacaaaa actgcgcagg caacggcaca ccgcgttcat cgcgccagtg
    15241 ttcatgagcc tcaacgccgt cgcgccgaag cttgacgacg acgttgtccg tataattgtt
    15301 gcggttggct tccagaatgg catggatgaa agatgccgtt ttttcagggg agacctggtc
    15361 ggacttcatc tggttcgcga gcgtgagcgc gaagatccag taggagacga ctgcaacgaa
    15421 ggcaaaggcc ccgcctccaa gcgcccacag caatggtttg ttgttcatac agttgatcaa
    15481 cctccggtca taaattcacg tggctataga ctctggaggc ggcttgcaag gcgattccgg
    15541 gagtcgtcag ccagccgcct cgaataaaaa gtgattctag agcgttcagc aaggtcaaga
    15601 cgtgggcctc cgtcgacgat tcacccatca ggccgatgcg ccagacctta cccttcaatg
    15661 gtcctaatcc tcccccgatc tcgatgccgt agttcgtcag caactctgcg cgcacctctg
    15721 cttccagaat gtgagaggga acagtcacgc agttcagcac cggcagccgc tggcctatcc
    15781 gaggaagggg agtcagcccc agttcttcca gcccggccac cagcgcatcg ctgttcattt
    15841 gatgacggtt gaatcgggcc ggcagtcctt cttcttcaac gagccgcagc gcttctcgca
    15901 gagcatagag catcgagatc ggggcagtat ggtgataggc tcttttccct tccgcccaat
    15961 agtcggcaat gagactcata tccaggtacc aactctggca aggggtgcgg cgttgcttca
    16021 ccaccgcaag cgctcgatcg ctcatcgtaa agggcgcaag tcccggcggg cagctcaggc
    16081 atttctgtgt gccgctgtaa cagacatcga tgccgaggcg atcgacatcc accggcacac
    16141 cgcccagtga cgtgacggca tcgacaacga agagggcgcc gtgctggcgg cagatcttgg
    16201 cgatgtcatc gagtggttgc catgcgccgg ttgatgtttc tgcatgaacg accgccacgg
    16261 ctttgaccgg ccctgatcga cggagggcct ggtcgagggc tgaagggtcg aggcattcgc
    16321 cccagggcgc ttccacgcgg atggctttcc caccgcaccg ctcgatgacc gaggccaatc
    16381 tgcctccgaa cacgccgttg atcccgacca cgactgcatc gcccggttcg acgaagttga
    16441 cgatcgatgc ctccatgccg gccgatccgg taccggaaat ggcaatggtg aagcggttgc
    16501 tggtttgaaa aacgtagcgc aagagggttt gaacgtcgtt cataaccgtg agaaagacgg
    16561 gatccaaatg gccaagaagc ggcgcggaga gagcccgcaa gactcgggga tggaccatgc
    16621 tcggcccagg gccgaggaga agtcgacttg ggggagtaaa gtcctgaagg gtcatacgac
    16681 tccgaataaa gaaaacatag tatagcggag ttttatgcca aggggccatg agtttatgtg
    16741 cgacgagggc cgaggcctcg ttacttttga ggggtttctg gctcgaaaaa ggccatgtta
    16801 tagttgagct tacttatgga tgacttcgta acaccatcat cccaaccgcc atcgtcccgc
    16861 tcctggtggc gtgagaccga ggctcccccc gcagtcgggc agtttccagc gcggttttct
    16921 ccgctgatca gctggcttgc cgcgattttc ttggtcgcgt cgttcgccgc cgtggtggtg
    16981 atggcctcct cgacgccgaa aatcgatcgg atcgaagcgc ccgaacgagc cctcagcctg
    17041 atggtgggtc ggacaatgga ggtgcaggaa ggactcaagc gtgcaccggc atgggagcaa
    17101 gagtggtttg gttgggtctc cggcaacggg gaagccgagc aggcctatgc catcgaatgg
    17161 tatcgagaac tgtcaaaaca ttcggacgat ccggtcgtcc ccttggatct ggccattctg
    17221 caagcagaaa ccgggcagat ctcccaagcg cttctttcgg cgcatgagtg ggacgaacgg
    17281 gaagatccgc ttccgcgatt cgcagaactg atccgtgcgg cctacagcga ggaccttccc
    17341 cctgatcgag agacgatcga ggcgatgcaa gcggatttgc ctgaactgtt gccgcaaggc
    17401 tggttttacg atcgcttggc gatcaggttg gctcagcgct ccaacaatcc ggagttagtg
    17461 gccagacttc aagaacaggc cgccatccaa gccgacaaat tgtacgcccg ttccagaaaa
    17521 ctgacgctgt tcgaactggg tgcgatgctg ctcgggactc tgctgttgtt gttcattttg
    17581 cacgggcgag cagccgatgc caatcccatc cgacttcatg aaccggggat tccaccacct
    17641 tggcccggcc gcgtcggcgc ggcagtgtta ctgcgcggcg gcgcgatcgg ggccgtggtc
    17701 atggccgtct gtctcatgta cctgccgccg gagaatgcct cattgagggc gctcgcgatt
    17761 cctatgacga acgtgccctt gctggcctta gcctactatc acctgttcag gcctgccggg
    17821 atgaccttcg aagaaggttt cggtttgagt cttccctggg ggcacttcgg ccggcttctc
    17881 gtcgcggttc ttgcggttgt cgctgccggt ctgtggggcg aatgggttat ggctcgcctg
    17941 tcggaacagt ggcatctggc gagccactgg actgaatggt tcgatggaga tttggtctgg
    18001 ggatctccat ccgtcaccat ggtcagtctg ctggagtatg tcatctttgc gccgcttttc
    18061 gaagaactcg cctttcgagg tttgttgttc gccattctgc ggcgcaagtt cagctttctc
    18121 cctgccgccc tcatcagcgc aggcattttc gggctcgcac atggctatgg tgcggtcggt
    18181 ctgatcagcg tctgctggag cggcgtgctt tgggcctgga tctatgaaaa aaccggaagt
    18241 ctcgcgcccg gcatcttggc ccatgcgatc aataatctct tggtgtgcct cgccgtcatg
    18301 gcgctgctgc gctggtagtc ctttatcgct cgagtccttc caaagcggtg aagagatcga
    18361 agtgttgaac gcgaacacca agctctgtgg tgagcttgtg cgtgttgatt cgttttccag
    18421 atgcgtccgc ctgtgattct gtccgaaggg ggacgttcca tcgttcatgc gcggtggagc
    18481 agatgacctc ccaagtgtgc gggtggccgt cgctcacgtt atacgtctct cccggttttc
    18541 ccctctccaa cgccgcgaga caggtcacca ctaagtcatc cacatgaatc agattcacgt
    18601 attttcgtga gggcccgacg cgaccgttct tgatccagtc aagcggattg cgccccggtc
    18661 cgtaaatgcc ggccacgcgg agcacgatcg catgacactg tgtccgcaga aactcctctc
    18721 cctgcacgcg cgatttactt cgatcgatcg gagccgattc gtcgagccag gccggcggat
    18781 aattgtgcga gtcgccgacg tcgtaggccg acgtgctgcc gagtaggacg atccggcggg
    18841 agggagcctt tatggcttcg gcaaactgat gaaccagttc gagtggggta gcgggaaagc
    18901 accacagcag atcggctgcg ggaggtatat gcgcccaggt ggcagaccgt tccaggtcga
    18961 actcgatccg ttgctgtgcc ggaacgtcag gtaggtgctg ctccgggctg cggcttgtga
    19021 caaatacgta tgggtattgc cgagcggctc taggcgcgag gaagcggccg gtatagcctg
    19081 agccgagaat gacgagagcc cggttatcca tattggtgcc ggcctgtttg aaggtcccga
    19141 cagtctgcca gtgaccatcc gccagagtcc attgaaaaca tttcgttctt tgtttggcac
    19201 actgtttcca tggtgatata aacaatgtac gtgtgaataa gtgcggttgc caaacaggac
    19261 ctacggtggc tctatgacca gcatgagatc gctccgcttc ctggcagcgg cttgcctgat
    19321 gcttgcggga tgtaacaacg gcggtgatgt gaccggcgga agcattaatc tttccgcaag
    19381 cggggctgtc gtgtatcttg ccgatcaaaa taccgtcggt gtcttcgagc tgtatttggc
    19441 gtcgtccgga accaagctta ctcctccgct aacccctggc ggcgatgtga tcagctttgc
    19501 cctgacgccg gataaaaccg ctgtggtgta cattgcggat caggatacga acgacgtttt
    19561 cgagttatat aaagttacca ttgcgacccc cggagtgagc gtcaagctta gcggtacctt
    19621 ggtatctggc ggagatgttt cgagtttcgc gatcacgccg gatggaacgt cggtcgtcta
    19681 tcttgccgat cagcagattg atcaagtgtt cgacctatac cgggtactgt tctcgaatcc
    19741 agcgtcgagc accttgctca accctgcatt gcctagcggg cggactgtca cgcaatttgc
    19801 cgtgactcca gatagcacca gggtcgtcta tattgccgac caaaccacgg acaatgtaaa
    19861 cgagttgtac caggtggcat ttttgaatcc ccccagcgac aacacaaaac tcaactcatc
    19921 gttggtggcc ggaggaaacg tcgtcaactt cgccatctct cccaacagtg cctcagtggt
    19981 ctatttggcc gatcaaacca tagatcaggt ttttgagatt tttctggctg cggttaccgg
    20041 accagggagc ggaacgagcc tgaatccacc tctggttgcg ggccaaaacg tcaccacttt
    20101 tgctgtgacg cccgatagca cagcggtgat ctatcgggcc gatgaggaca caaccggcac
    20161 gttcgaactc tatcgagtgc tattcctgtc tccccaagtc agcgtcgtga aattgaacgg
    20221 gacgttgacc gcgggaggaa atgtgtccag tttctccatc gcggcaaacg gttcggctgt
    20281 ggtctacagc gcggatcaga ctattcttgg ggtggtggaa ttgtttcgta cgccgcttgc
    20341 accggtggga ccggcacaaa aggtcaatcc cacttatgta gccggccaaa atgtcacggc
    20401 gtttgccatc acgccggaca gctccgcggc gatttatatg gccaatcaga cctctgccgc
    20461 tgtccagttg tatcaggttt cattttcatc cctgcaggtg tctagtccgc ccctcaacgg
    20521 ggctttggca tccggtggga acgtggcgag cttttcgatt tcaccgaaca gcacgcttgt
    20581 cctctatctg gccgaccaga cgaccttcgg taccgaggaa ctgtactccg tgaatgtcgg
    20641 agctcccggg acatcgtcca aacttaacgg accgctggtg cttgggggta acgtcacggc
    20701 tttcacgttt tgacagtttc accgtacaat attcctcggc ccctgctgaa gtatcctgtt
    20761 tcccgtgatt tggtgattgg aagctggccc gtgttatcat gacgggccat gccgctcact
    20821 cgattccatc ctctcatcgc tgagtggttt caatcgcatg tcggtcagcc gactgatgtg
    20881 caactacagg cctggcctgc catccaatca ggggccgatg cgctgatcgc cgctccgaca
    20941 ggctccggca aaaccttcgc cgcctttctc tcctgcatcg attatctctt caaacaagcg
    21001 ctcggccgtg aactggatga ccatactcag attctctacg tctcgcctct caaagctctc
    21061 agtaacgaca ttcagaagaa tttgcagaag ccgctggccg acatcgggca ggccgccttg
    21121 caagccggtt tgttgatgcc ggagttgcgc gtgttggtac ggaccggcga tacgccgatg
    21181 gccgatcggc agcagatgct caagcgcccg ccgcatattc tggttacgac gccggaatcg
    21241 ttgttcatcc tgttgaccgc ggagaaaagc cggcgactct tgcagactgt ccggacagtg
    21301 attgtcgacg agattcatgc gctcgcgccc aataagcggg gcgcgcacgt ggcactgtct
    21361 ctggaacggc tggaggcgct gactctcgtg aagccgcaac gcatcgggct ctcagcgacg
    21421 cagcagccca tcgaactggt tgcgcaattc ctcgtcggcg ccggcgttcg aagttcgaag
    21481 tgcaacgtgc gaagttctcc gattccggaa acctcgaatg gtgaacctcg aatctcgatc
    21541 tcgattgcgc cctgccggat tatcgacgtc ggtcacaggc gcaagatgga tgtggcggtt
    21601 gaggttccga aggacgagtt gagcgcggtc gcgacgaatg cgatctggtc tgatatctac
    21661 gatcgggtcg ctgcgttggt ccgtgagcat cgcaccacgc tggtcttcgt caacactcgc
    21721 cggttagccg aacgtgtgtc acaccatctc gaagagcgcc tccaggatct cggagccgat
    21781 gtggtcgcgg cccatcacgg cagcctttcg cggcagatcc gtctttcagc tgaagagcga
    21841 ttgaaatccg gcaagacgcg cgtggtcgtg gcgacggcgt cgctcgaatt gggtatcgat
    21901 gtcgggacgg tcgatctcgt ctgccaaatc ggttcgcccc gctccattgc cacctgcctg
    21961 caacggatcg ggcgtgccgg acattggatc cacgccattc ccaaaggccg gctcttcgcc
    22021 acgacgcgcg atgagctgct cgaatgtgcc gcgctgatcc gggcgatgaa gggcggccag
    22081 ttggaccaca tcgtggtgcc tccggagccg ttggatattc tggcccagca gatcgtggcc
    22141 gcggcggcga cgcaggcctg gacggaacag gacctcttta cactcgtccg acgggccttt
    22201 ccctatcggg acttggaccg ggccacgttc gatgccatcg tgcagatgtt ggccgacggg
    22261 attgcgacca atcggggacg aggcctggcc tatctctatc acgaccggat caatcatcga
    22321 ctcaagggac gacgaggggc gagattggcc gcgattacgt caggcggggc gattcctgac
    22381 acggccaact acgctgtcgt ggcagagccg gacggcaccg tcgtaggatc ggtcgatgaa
    22441 gattttgcag tcgaaagtct tgcgggcgac atcatgctgt tgggaaatac ctcctggcgg
    22501 atcaaaggcg tggagatggg taaggtgcgt gtggaagatg cccacggagc cccgcccaat
    22561 attccatttt ggcgaggcga agctccatcg cgaacggcgg aactctccgc tgaggtcgct
    22621 gcgctacgac aagaaattgc gcagcaggcc agtggtacga ggcaagaggc aagtggggat
    22681 gagacagttc ctcgcgcgcc cctcgcctct cgcctttcgc ctctcgcctg gctcaaagaa
    22741 gaatgcggcc tcgaccagcg aggagcccag caagcggttg agtatgtgtt ggcgggaaga
    22801 gcgatcctgg gaaccgtgcc gacacaagag accatcgtgg ccgagcgctt tttcgacgag
    22861 agcggcggga tgcaactggt gatccatgcg cccttcgggg ggcggatcaa tcgagcctgg
    22921 gggcttgctc tccgcaagcg cttctgcgtg acgttcgact tcgaactaca ggcggcggcg
    22981 acggataacg gactcgtcat ctcgcttggg gagaaacata gcttcccgct ggaatcggtg
    23041 ttcggctatc tccattcgaa ctcggtgcgc gaggtgctca ttcaggcggt gctgctggcg
    23101 ccgatgtttg caacgcgatg gcggtggaat gcctcgcgct cgctggcgct gctccgattt
    23161 tccaacggca aaaaggttcc gccgcagatc cagcggatga aggccgaaga cctgctggcc
    23221 gcggtgtttc cggacgcgat tgcctgccag gaaaatctga ccggtgcgcg tgccacacga
    23281 cagattccgg atcatcctct ggtgcaggag accctgcggg attgtttgac cgaagcgatg
    23341 gatatcgacg ggctgattga tgtccttcgg cggattgagg cggggaccat cacctgtgtg
    23401 gcgatcgata cgcctgcgcc gtccctcttc tcgcacgaaa tcctgaatgc caatccctac
    23461 gccttcctcg acgatgcccc gctcgaagaa cgaagagcga gagctgtgga gctacgcagg
    23521 acattgccgg ccgacgtagc ggctggaatc ggtgcgctcg atccggaggc tattgaagaa
    23581 gttgcccgtg agtcctggcc ggtggttcgg gatgccgacg aactgcacga cgcgctgctc
    23641 accttggtat gggtgccatc ctccgaagct agcgagtgga gtgcgtatgt gccacgcttg
    23701 gttgaggagg gacgggttgt tgaactttcc gtaaggggtg aggggttagg ggtgaggggt
    23761 gatgggcgag aggtgaaagg gtgggtcgca cgggagaatc aggaatcggt cgagcaactg
    23821 tacgtccgag ggagtgagcc gacgctcgat gccatcgtgc aaggctggat ggagagcatc
    23881 ggcccaacca cgacaggcga actggctgag cgtcttcacc tatcgagtga tgcggtgaat
    23941 gaatcgatgg tccggctcga atctcaaggc caagtgcttc gcggtcaatt ccgtgagcgc
    24001 cgcggtgagg cgtcaggcgt caggcgtgag gcggacggga tatcgccttc cccctcaccc
    24061 cttacccctt acggtgtgga atggtgccat cgtcgtgttc tcgcgcgcat ccatcgtctg
    24121 acgatcggca aactccgcaa agagatcgag ccggtgacgg ccgccgagtt catgcgtttt
    24181 ctcttgcaat ggcagcatgt ggcttccggt tcgcgccacc atggagagtc aggcctgctg
    24241 gaggtgatca aacaactggc cggattcgaa acggcggcat cgagctggga tacacaggtg
    24301 ttgcgggtca ggatggcgaa gtacgaaccg gaactcttgg atcggctctg tttaagcggg
    24361 gcggtgagct gggggcggct gtctccgcat ccgcggctgg cgcaatccga tggcccggag
    24421 cgggccaagc ggatcatccc gaccagtgtc gcgccgatta gtgtgttccc gcgtgaggac
    24481 agcgagtggc tgctggatac gtttcaggac gggaccgggc aaggtcccga accttttgcc
    24541 gcgttgggcg cagtggcaca ggaccttcgt cgcgcgctgc aggaacgagg agcctgtttc
    24601 ttcgccgacc tcgttcgagc cacagggcat ttggcgagcg aggtcgaaga agggctctgg
    24661 gaattggtgg cggccggctt ggtgactggc gatggcttcg acaatctccg tgcgctcatg
    24721 gatccacatc gccgtcgcgc ggaagggcgc gagcgcacac gtcgtccacg acatgccgcc
    24781 ggtcgctggt cgatcctgcg caacgccagc agccatcagc cgtcggccgt cagctccggg
    24841 tccgttagtc cggtcacctt acgcctcaca ccttacgcct cacgctcggt tgagctcgtt
    24901 gcccgccaac tgttgcgacg gtacggcgtc gtgttccgcg acttgctcgg gcgcgaaacg
    24961 ctcgtgcagt cctggcgcga tctcctggta gcctatcgac gaatggagtt aaagggcgag
    25021 gtccgtggag ggcgctttgt gtcggggttt gtcggcgagc agtttgccct accggaggct
    25081 gtggaagcac ttcgtgcgat caggaagggc ggcggtggca cgagcgggct tgaggtcaag
    25141 ctgtcggcct gcgatccgct gaatcttgcc ggtgtgattc taccaggacc acgcattcca
    25201 gccgtgccga cgaactttct cgtgttcaaa gacggtgtcg tcgctcgtac cgtgctgggc
    25261 cgcgagatcg tcgaacaatc ggattcgaca gtcgcgtggg cccgtcggca tgactgagcc
    25321 ggcggaccca ttgaggtgcg gtgattatcg ggacaaggtg cgcaactgct ccaggcgcgc
    25381 ctgctgatct gctgttttca attgagcaaa gcgatcctgg gcggcagctt gaaatgtcgg
    25441 ctgagcctcc ggcgccacgc ctaacagagt gcccatggac gtcagatatt cgccctgtcc
    25501 tctggccagg tcttgttcca ggttcgcttc gttatacgtc gcaaaggcaa tggctttgtg
    25561 ttccgggttc aacagcccgt cttcattcca ccagatgcgc cccgaggttg tgcctgtgat
    25621 attcgatgtc gtgtcggttg tttcattggt ggtgcccttc aacgtgcagc cggcggagat
    25681 catcaacagc gcggtcatgg cggcaaacat caagcagtag gtgcgaagct gtttcatggt
    25741 tagcctcctt gcactgagta aggttcatat cctgcgtccg gcttttaact ataacaggaa
    25801 ccacacggct gtactatccc cggaccgtgc aaagcagaaa tagcggtgca ggggaggcat
    25861 ctcctgcacc gcggaagcag aacttagttg ctccataacc tctggtacca cttcttctct
    25921 tcgacaggag gcaacgaagc aatattcatg gattttcgga tctcgccgat gttccagccg
    25981 gtcagtgttg ccagagtttg tgcttcccgc tcgtaacgct cgcgcaactg ttgacgataa
    26041 gccgttgcgg tggggcattc atcggagccg gtccaaggat ggcgcaaaat ataacgggcc
    26101 tggaagtttc cccggtccga cgtttcctgg aatatcaggt cctcgggaaa atgggctgca
    26161 tcgtagcgga cgtggagccg ggtcaggaaa acattctgcg ccacaggcat catcttgccc
    26221 atccgcccgg cgttgtcttg ccagaagaca cccagactac gaagctcttc agcggagagg
    26281 ggattggccg cgcagggatc gcaccagttc atatcccagg catattccag aaacacacct
    26341 cgttcatttt cacgcttcac ttgctgcgtg aatagatcgc gatagaagtc gccgaactta
    26401 tccttcacat acagcggaat ctcctgtgct tccggcaatc tgacggtgcg atagttggtc
    26461 gtctcgacgc ggccctgttt cgtcagaaaa taaatgaaca gttcctgagc cccatcggca
    26521 ttcaccgtgc cgaggcggat cggcagaatg aacttgggcg actcgaaggc gatctgtagc
    26581 gggcgcaggt gtgtgaagcc cagtttagat tgctccgcca gattcacctt ggcgacgaag
    26641 aacttcatcc cctgtttgag ataactctgc aggacagagg atgccccgtt tggaatgcgg
    26701 tagccgtttt cgctcagcca ggtttcaaga ccggcgcttt ccttcgccga gaggatcaga
    26761 atgtcgtatt caccgacggt gtattgcgcc tcgaccgtca cgcccagggc acggtcgcgc
    26821 tcgggggctg ccgccgatgc cggcgccatg ttcttcatgg cgtccctctt ccgttccatc
    26881 aattcgtact tgatgcaggg attctcgtcg aagtactcga cgagtcgcgg cgccgagtag
    26941 tcggccaggt gtttgagtac tgccggatta ccgatgtgga tctgttcttt ctctaagacg
    27001 gtcggcaccg gtaccaccat cgcgaactct ttgacctcgc ctttgaagtc gttcgccatc
    27061 gtgatgacgg ttttgttgtc gtgtcgggca atagccacct cggacgcctt gttgaagagc
    27121 ttcgtatcgg ccttgccaac atagaatccg caaaaggcaa aggcggtgcc attcccgacg
    27181 agaaagatca caaggagtgc caaggcaagg acgtagaagc gtctattcgg catacgcggg
    27241 tcctgtcgcc aaccagccca tccgtcctcg caccccgtcc cgtgtaggga tcgagtggcg
    27301 gcctggtcgg tccctgtgct cgcggaacgc gacacgcggg aacttatcga atgtttaaat
    27361 tgcgggcggt gttcgttcga cgcgcgcacg gtgggaccaa tccaggccgc tcttgattcg
    27421 gggagcgaaa aacccggagg aggcggatgg gctcccaggt tggcgccacc cgcacccttg
    27481 ggatgaatgt taagtgtctc gtcgtcatgc ctactcatga gatcctcctt tgttgtgaaa
    27541 tggacaaggg aacgagcaca gggagggaaa tggagacaga taatttaccg ctcgagggtt
    27601 ttgaccagtc ataacgaaca ccgggcagca agtagtccaa cagtggcacc agcgggaaac
    27661 aaacaatcag cccccagagt ggaccgttcg gacggaacaa gccgaactgg acgtagagtg
    27721 ccgcgagcgc gacgaacagc gcgaagagaa tccggccgct tcgtgaatct ggcgtggtct
    27781 tcgggtccga gatcatgaaa aaggcaaaaa tgagcagcgc gccgctttcg atctgatgca
    27841 ggggaatcgt cagcggatca ccgagccaga tcgaccttgt gaaaagcagg ccgacgtaga
    27901 aggtaagaaa agccagcgtg acgtccgctc gcgcggcgcg ggtgacgacg agactcccaa
    27961 ggcaggcgat cagaaatccg aaccaggcga cctgtcccca ttgccctggc gagatccagc
    28021 cgagtccgcc ggctagtacg acgaccaacg cgagattggt gggattaaga agatgcttct
    28081 ccttccagcg gatgatgaat ttgctgccaa tggcaatgaa cgaagccagc gcggcgacga
    28141 ccagatcgtt ggtgcggagc aacagacaca gcgacagggc ggagatcaga ggactgcggg
    28201 gatcaaacgc acggagtttg aaatagcgtg tcccgacata ctgcgtcagc agggctgtgc
    28261 caaacgtgat ggcgatttgc caaggggaga cgtcgaagtg cagccagcct aggccataga
    28321 ggaggagtgt gctcaggctg gcaatctggt acaggcgggg atcgcgccag ttggctggtg
    28381 tgttcggttg gctcttgccg ttgtcttgcc ccatggagcc ctcccttcac aggaggatat
    28441 ggggcgaggt tctgtttcct ttcagggggc tgagcctttt cgcagggcct gttggcgagc
    28501 actgctcgtc ctcgcacccc tccctgtgta gggggaaaaa ggggtgggcc attagtctcc
    28561 tttgctcgcg cgacgcgcac accaggccaa tcgaatgttg atcaaagggg gcggtgctcg
    28621 ccgcatgcgc gcagttggag accaattggc ccaccccctc aggatgcggt agccgcgaga
    28681 ctgggaggag cattgagagg tgtcgggcgc ccgctcgctg ccaccccgca gtcattgcag
    28741 gctagaactg agcgttgaca agcgattgag cacgcgcaag aatcacgcgc ctgccagaag
    28801 tatttcggtg ggtactattc cgcttagcat aatcaatagt taaccctgaa aaggtttcaa
    28861 gaaccggtgg ttgccctaag aacaagtaga atgcctcagt ttaaaagttc gagaacctgc
    28921 cgattgcgtg aataacatgc gacaagcatt cattatcatg cagatcggca atgcacagct
    28981 cgatgctatt tgcaaagacg caattgtgcc tgcacttgca gcatgcggct tcgatgcgaa
    29041 gcgggtcgac aagcacaatg aaggtggatt actgaagagc gagataattg ggttcatcga
    29101 gcggagcgag atcatcgtcg cagatttgac taacgaaaga cccaactgtt acctcgagat
    29161 cggctatgcc atgggtatcg agaagttccg caacctcgtc cttaccgtcc gcgaagatca
    29221 taatccagag agtcctagct acgttcgggg tgggcctaag atccacttcg atctcgccgg
    29281 ctacgacatt ctcttctggc atcctgacaa ccttgatgcc ttccgtgctg aactcgaaaa
    29341 gaaggtccgc cggcgtctgg ctgtcgttgc ctctccgcca gctgtacccg cgttgtcctg
    29401 ggatgaggag tggattgcgg ctcagcgcca agttgccatg aaaggtttat cagcggctgg
    29461 caagaaggct ttcatggagg cccggttcgc cctggaggtc ccaaaaccca acaagacaca
    29521 acgcgagctc gatgaggctg cgaggcaggc agaaatccat acctttggtt ggcccatcgg
    29581 tgcctatctt gggaaccgag ctgactgccg cccacgccct cgcgccgatg gaattgttgc
    29641 ggagcttgca ttcaaagaca ggtcctctta cgactattgg gcggttcggc gtaacggaga
    29701 tttctatttt ctcggctcac tgttcgaaga cgagcgtgac ccgagtaaga tattcttcaa
    29761 cacgcggatc gttcgcgtta ctgagacctt gctctactgt gcgaggctct attctcgcct
    29821 cgagatcgac ccaaccgttt tcgtcaacgt ggcagtcagg catggtggcc tcaagggacg
    29881 cttcattggg tcttctagcc cgggacgcat tctctcgccc cgtgccgctt cgacagaaga
    29941 tgagattgac tgtgaggtca ggacttcact tcaagaaatt gagtccaatc tcgtacctct
    30001 cgtcaagcaa ctcgttgcac cggtcttcgt gctcttcgac ttcttcgagt tggcggattc
    30061 ggtctacgag cacatcgtca acagtttcgt ggatggcagg gtggtgtgaa aagacaaagg
    30121 ttccagaacc attgattcat ccctccgtat agcatgaatc catttcatcg atggtgcctc
    30181 ttacgttcta caaggttaga tttgtggcga ggagaggaaa tccgtggcgt ttgaagatct
    30241 gatccggatt ggctcgtacc tgtccgtact gggcgtcatg gctacgtggg aactgctggc
    30301 accgcgaagg atgctgacag cctcaaaact ctgccgttgg ggcggcaatc tcacgatcgt
    30361 gatcttgaat acggctatcg cccggttact ttttgcggga ggcgttgcgg cgacagcggc
    30421 gatagcgcag gaacggggct gggggttgtt gaactgggtc gacgcgccaa catggctcga
    30481 attcgggctg gcggtagtgg cgctcgatct ggtcatctat gggcagcatc aggtgtttca
    30541 tggggttccg atcctctggc ggttccacat gatgcatcac tcagatctgg atctggatgt
    30601 gtccagcggg gtgcgatttc atccggtgga gatcatcatt tcaacagtgg tgaaaggggc
    30661 ggcggtggtg gcgctgggag ttgctccttt ggcggtggtc gccttcgaga ttgtgcttaa
    30721 cagcacggcc ctgttcaatc acagcaacgt gaagattccc ctggctttcg aaccgattct
    30781 ccggtggttc gtcgtgacgc cggatatgca tcgcatccat cattcggtgg atccccgcga
    30841 gaccaatagc aactacggat tcaatgtgcc ctggtgggat cggctgttcg gaacctattg
    30901 cgcgaaaccg gccttgggac agcaggggat gaagatcggc ctcgaacacc ttggtccccc
    30961 ggtctgcttg aatctgttca tgatgttgca attccccttc gtggcgcagc tcgggcgcta
    31021 tgccggtcgc actcaaccgt gattcctgcc aagatagaac ctagtgaatt catagattga
    31081 aaaacttatc cgacttcatg cagcttgatc aggtttgtcc cgcccggctg cccaatgcgc
    31141 gtccccgaga gaatcacgat ccgttctccg gttttgacca gcccctctgt tcttaaccgt
    31201 cgttcggcct cgtccacgcg tgcatcggtc tgctcgattt gcggcatcgt ataggggcgc
    31261 acgccccaat agagtgccat tcgttgcctg accggctcaa acggggtgag ggcaataatc
    31321 ggcgcggctg gccgttgctt cgagattaag cgggccgtca ttccttgctc gctgaacgcc
    31381 acgattgccc cggcgttgat cgtccttgcc gcagaagagg ccgcggtaca gatggcttcc
    31441 ggaaaggaca gttccccttg ttccccatcg gccttcggca gccagcccgg cttggctcct
    31501 tcttctgctg cccgaatgat gcgatccatg acctggaccg tttcgatcgg atgcgagccg
    31561 atggctgttt cggccgagag catcacggcg tcggtgccat cgaaaacggc attggcgaca
    31621 tccgaggctt ccgctctcgt cggacgaagt gccgacgtca tcgactccaa catttgcgtc
    31681 gccgtaatga caaggcggcg accgcggttg gcttccgcga tgatgcgttt ctgtaaaacc
    31741 ggcacggctt cggggcccat ttcgacgccg agatcaccac gggcgatcat cacgccgtcg
    31801 gcctgctcca aaatctcgtc gaggttagcc accgcctcgg atcgttcgat cttggcaatg
    31861 atcggcagat tcccgccgca ctcggcgact aactttcgcg ccgctacaat gtcctcggga
    31921 cctctgacga acgagagggc caaataatcc acgccctgtg cgatgccgaa ccgaatgtcc
    31981 tcccgatctt tgtcagtgag ggtaggcgcg ctgacacggg tgcccggcag attaattccc
    32041 ttggtcgacg tgatcctgcc gccgatggta acggtgcatt cgacggaacc gccggtgatg
    32101 cgatcggcaa tcagttcaat aagtccatcg ttgatcagaa tcctcgcgcc aggttggatg
    32161 tcgcgtgcga ggtattggta cgtgactgga atctcgggaa cggcggagcg agcgatggcg
    32221 acggattgaa cgccaagctg tccccctgac cgtgctaata tggtctgtag gtgcacctgc
    32281 tggccggctc tcacctcgat tccctcgtcc gccacggtgc ccacgcgaat ccggggtcct
    32341 tgcagatcct ggatgatcgc caccgccgca cggcgacgct ctgcggcgta acgaatcgct
    32401 gtgatagcgg ccgcgtggga ttcatgggtg ccgtgagaga aattcagccg ggcgacatcc
    32461 atgccgctct caatgagccg gtcgagcatt gccggcggat tgctggccgg accgatcgtg
    32521 cagacaatct tggcttttcg catcatgtgt tcctcagctc ttgacaagat attaacggac
    32581 acccaatgat gctctcgtcc attccgttaa tgctcgccac cggcatcggg cggcgcaaac
    32641 cgccgagggg gtgaagaggg tatgcagatt aacacagatc cgctcgattt cgtgacgatt
    32701 gagggtttgc tggcttacgg agaagagttc gcgcgacgtc cgtcacagga cggagtgctg
    32761 ttgccgcctg ctccacctcc ctcaaacgac cgagccggcc gagcccgtgc cgcgatggag
    32821 aagatcgcgg cgtttgtcca aaccgcccaa ggcggatttc caggagccga cacctatcga
    32881 gcggcaagga aagccgtcat tgatgaagcc tgtgccggtg atgacctggt gttttttgcc
    32941 gcatggaacc ggctgctggc ggaaggaaag ctccagccct tgttgcgggc cccgatcgga
    33001 tcagtgttga agccgactcg gcgccgtccg gtagcgatcg tcccgcgcgc gcagctcacg
    33061 ccgcagctcg cggaaggacg catcgtgctc gacttaggcg atgagcgatt ctggctgttg
    33121 cctcgcaatt tggccgatcg cactctgctc tttacgatgc gccacggcat ctctcgcgtg
    33181 gagagtaaaa cgcatcgggt cgggcgtcgt ttggcgaatc aactcgatcc ggaacggggc
    33241 ataccgcggg ccgatgccgt gggggccgcg ctggcccgga tggtcggcgt ggtaggccag
    33301 caactcgact ttctccattt gactaattac ctggatccgc ggacgtttct ccacttcatc
    33361 agcaggagtc ccaacacccg gcagctcttt gaacgcgtgg ttgccgcgct cgtccaaggg
    33421 ccgacggctc ctcccgaacc tgtgtgggag cccgcgctgg agtcgtcgga ttttggctgg
    33481 cttaccggcg tggaaaaaac ggtggaggct caggaactcg ccaaggcttt tggagtcgat
    33541 gtgcctatcg ccaaacgcct gatcaaagat ccgctgtatt gttatcccgg cggcaattcg
    33601 tttttcgatc tctacgtcga tgtgattgac gggcttcacg ggctgggcga agccaatcgt
    33661 ggcaaagtgg cctgcctgta tacgcacagt tcgacgctac gggccctcat gatctatttg
    33721 gacccgcgcc cgtttcacga agcgttcaac gaattcagcg actacaaaga gagtcaggac
    33781 aatgtggtcc tcctgacgtt cgaaaagggg cgcttgtccg gctattccac ggcggtcgga
    33841 ttgtcggagc gggagagggc ctcgcgggat tcgtgggttg cggtcgaacg ggtacggaag
    33901 gatcgggttg cactgaaacc tggacagatc aaacggatcg tggcattggt ctcgggagga
    33961 gacttcgccg gtgccggcgc cgccctgaag gagttgcgcg tggcaggcaa ccgattcggc
    34021 ctcgacgtcc acttcgtccg gcacggattt ctcgggcttg cgaacaactg gattgaaacg
    34081 gtgaccgaag aggacacgcg cggcatgagc agccatgcga gcagccccat cggcagcagc
    34141 cgctttgagg actttaagga ggaacaggtt cagcaggccg cgatgcgaca cctcgcgccc
    34201 tttctgaaag acgcagccct ggtcgtcctg ggaggtgacg gcagcctccg gggcgcgagg
    34261 gctatctatg aagggttcgg catccaggtt gcgggcattc ccggcagtat cgacaacaat
    34321 atcgaaggca cgacctcgct gggctaccat tcggccatcg ccctggcgga taagtcgatt
    34381 gagtctctca aggctacgag cgccgccatg ggaagcgtct tcttcgtcga agtcatgggt
    34441 gcgggatcag ggcatcttgc cctaggctgt gcctatcaag cgagggccga gggcttactg
    34501 gtcaacgagt atcccgatcc cgatgcctat atcgacgagg tcattctcgg aacgctgaag
    34561 cgaacattgg gggtgccaaa caaaagccac ttgttcgtgg tggccgagcg aaccccgcat
    34621 cgccatcatc gggagggcgg cgtgcacggc ctggtcgatt atgtggctgg cgtgattgcg
    34681 gaatggccgc agcgtcacgc gcgtcccggc cagtatccat tgaacctggc gaccaaggcc
    34741 accatacttg gacacacctt gcgtggggct cctccaacgc cggaagacaa agcgatcgcc
    34801 cagcatttgg cctatgagac cgttcgccgt ttggttgaac ggccggagga tgttgtcgga
    34861 tgcatgctgg cgtaccgtga gcgaggcacg attgaggcta tccccctcca tgcagtggcg
    34921 gccaaacaat tcgactggga cgcctttgcc aggatgcatg gcagtgagct gctcgagaat
    34981 cggtcctgac agacccgcag gccgttctgc agattccgca ggagggtcgt gccggatttc
    35041 aaggcaaact gagggagatt tcggtcgaga gctgagcagt tgacgatgag tcttgagtca
    35101 cgacgtaagt ggcttgttcc tgtgagttgg gtgctccgcc aatggattct tggaattggt
    35161 tgggagagag ccggccgagc atggcggctt cctgtctggt gatcgggccg attggaacac
    35221 gtgagagacg aactgtgagg gccgcggtgc cgatttcagg cgggagcgga acctgttgtc
    35281 gttgaccggc tgcaagcttg atagaaatct gcccgaattc cttctcggga aacaacaatt
    35341 gcggcgtgga tgatccctcc gttttccaga tctgcagata ggctggttga ttcgcttcta
    35401 ccgtcagtcg agagtgccct ccgctccttg atgccgtcgc ggcgtccact tcccggtctt
    35461 gtccatccgc tccacgaatg acgaagctat agcggagacc gagtgctttg agttgcacaa
    35521 cagcttcagc cgctttgccc gctgctgcaa atcgatccag cttctgttcc ggccggttgc
    35581 cttgcggggc ggattcagcc agcggtttca tcggttgttc tttttcttga gccgtcacag
    35641 gtccgtccgg gcgcgcgaca ggctcgccgt aaaaaagtgt gcgggcgctg actgtcgatg
    35701 cggagcttgc tgagggtttc tggtctgtcg ccgcggtcgg ttccgctgtt gttttcccga
    35761 gcgcagccac gggcggttcg ccctgcttgc gagctgcgtc ttgttcactt cgcagcgggg
    35821 cactgtcgcg ggcggcatca ggggtctgct gttgctccag cgccggtggc gcggcgcgtt
    35881 ctcgcttggc catcttgtcg gcgagtaggt tgtctttagc cttcgactcg ggctcagcga
    35941 cttggggcga cgtcggttga gatgtcgagg gtgctggggc tggcggagcc gccggcgtca
    36001 tatgttcggt cacaaccggt tgagccgctt gcttgagact gtcttgatag atcttggtcc
    36061 cgagtacgac ggcgaagacg gccacggcga gacttccggc aaaggcgagg cttgccggtc
    36121 gtctgaacca atccagccat gagagggaac cgcccgctcc ggacgggctg gtttgattca
    36181 atgcctgcaa aagccgacga cgaaccgctg gatcggacag caactctttc agcgcttgtt
    36241 cgtcggcgag cccattgaac aactgctggt catgaagcgc agcggtatag agcatgttgc
    36301 gctcctctgc tgtcaacgta ccagccgcaa atccccccag cagtttttcc aattcgtgct
    36361 cagacatgtt cactgcaccg taaggcgtaa ggcgtgagag gtgaggcgat gagtctcatg
    36421 tccattcacc atttaaacat tgtgcattgg gcgttcactc ccaagatcca cccatgaggg
    36481 agagcaattg tttccggcag cggaaatccc acgtgtagat cgtattgagg gaggtttgcc
    36541 ccatcagttc ttggatttcg gggaaactct ttccttccag cttccatttg aagagttcac
    36601 ggcagcgttc gccgagttga gctatggcgg ccaacagccg gtctactcgc tgtttctggt
    36661 cgagtcgcgt cgccgggtcg tcgccgggat cggcgagcgg gaggtcctcc accgattgct
    36721 gattatactc gccccggcgc agtgatttgc ggtgggcgtc gagcatcttg aagcgcagga
    36781 cctgaaaggc caggggaacc agctcggtca gttccgtgac tagggcgtat ttctcatgca
    36841 ggagcacgag cacctcttgc gtgaggtctt cggcctgctc cctcgaaact cgtaatgtcg
    36901 caaaggccag aatcctttcc cggaggctgg caagtatctg atctcgatcc atggtgcgtt
    36961 gaacgagcta tagcccgggt tgagccacaa tgtcgttgaa cgaccggaac ccgagcccca
    37021 acccccggca gaccgcgacc tgccggtcga agcgattggt ggtgcgaatc atcttggcta
    37081 acgcgcgtat ccccacccgg ccccaggccg ccaggtgaaa caccgacaga ggataatcgg
    37141 ttccgaagat gaggcgatct tgaaattccg gatgtcgcct gagatggagc agcatccgca
    37201 tgcgattcgg tagcgtgagt gcggagatgt cggcgtaaaa gttgtgatag cgcttcgcga
    37261 aatcatggaa ggttggaata aacttttcat agagcataag gccgtagctg caggcatggg
    37321 ctgcaatgac agtgacccct tcgtcgagcg cggtgcggag ccgatcggga tcgccgacgg
    37381 actgatcttt tccgatgagg ctgaactcga atcccacatg gctgaggaag gggagcttgc
    37441 gttgggccag cgcgcgataa aacggtctgt acttggagtc ggccgggtcg aaatgctggg
    37501 cattgggcag gactttcacc aggactgccc cggcctctgc acagcgatgg acttcctcca
    37561 ccgcgtcctc ccgttgaggg ttgatcgacg ggccggcaag gaagatgtcg ggataggcct
    37621 gcgcggtctt caacacgtag tcgttgctga taagaaattc cgtgtgctgt cgattgagca
    37681 ggccggtctg gtcgtagaac ccgtccatgc cgaggagcac ggccttgtga acgtgacgtg
    37741 aggcgcgcaa ctccgtgatg aggtggtcga ggtatttctg gttgctttcg cgcggatgag
    37801 ccggcgagag ttcgtgcttc ctcaagagaa aacgaaacaa cggacttttg agcagcctag
    37861 gtgaaatgta acagccattg tccccgtccg gcagggctgc gagatggacg tgacagtcga
    37921 tgagcgtttt cccagtcaat ggtccttatc ccccttaccc cccacgcctc accccttacg
    37981 ctcgccggcg agtcgttcca tggcgatccg tttcaggcca cgcaggtcca tcttaccagt
    38041 ccccagcact gggagagcct cgactctgac gaagttgctt cggggaggaa caaagaggtt
    38101 gggaagtcct tctgccgcga gtttcgccag gatctcaggg atgcgggctt cttcaagaat
    38161 atgcagcacg gccaattgtt cgcccttccg ctcatcggga ataccggtca cggcaaacgc
    38221 ttgtgtctca agccccgctg ccagttgcaa ggcctcttca actttcccgt gcgggaccat
    38281 ttctccgccg attttcgaga agcgcgacaa tcggtccgtg atggtcagga acccgtcatc
    38341 gtccagtatg gcgatgtcgc cggtaatgta ccagccgtcg cgcatgacct gggccgtgag
    38401 atcttctcgc cgaaggtagc ctttcataac gttggggcct ctgacaagca gcatgccggg
    38461 tgtgccggga ggcaacggga caaagctgtc cggttcgacg atcttgacgg aaacgcccgg
    38521 gaggggttga ccgacagtgc cgcgccgcga tgccggttgg taaaagccgg ccgcgcgaaa
    38581 gtccggagaa ttgacggcga tgacgggcgc acattccgtc acgccgtagc cttcgatcgg
    38641 ttcgatgccg aacttgtctt cgaccgtctg agccagtcgt gccggaagtt tttctgcgcc
    38701 ggtcagaatg acccgcaagg agccgaactg ttctggcgtg catcggcgtg catagagttg
    38761 aagaaacgtc ggggtggtga cgaggaatgt tatccggtgg cgtcgtatca cttccccgat
    38821 gccggccacg tccagtgccg atggatgaaa caccatggcg gcgtcgttga acatcacgaa
    38881 ccagaagacg agatagccga acgagtggaa gaaggggaga atgccgagca cgcgttcctt
    38941 ttgatagaga tgcagcactt gcgtggccgc ttgcgcgttg gagtcgacgc tgaaatgaga
    39001 cagcatgacg cctttcggtt ccccggtgct gccgctgctg aagatgatgg tcgcgagatc
    39061 gtcgatcttc aagacagtgc gttggccgca ggccagttcg atcagccgtc cgggagcgcc
    39121 cagcgcaaag agagccgcga ggagcttctg tgctcgcccg atcgtcttgg ccacgtcctc
    39181 aagccatatg atcgtcggtc cgtccggcag ttccagcttg gctttttcga taaaggcgcg
    39241 gctggtgacg acggtgcgca gttcagccag tcgcacagcc gcttcgaggc cggacttgcc
    39301 gactgtgaag ttgagattca cgctggtctt cccgcacaaa ggcgcggcca cattgaccag
    39361 cgcggccgcg acgctcggag ggaggagaat tccgacattg ttctggccgt cccaatagct
    39421 acgcaaggct ctggcaaggg caatcgatcc gatcagcgct tgcagggagg acacacgggg
    39481 gcggatgtgg tcggccatga cgaaccggaa gggccgcctt ctcatcgcgc tgatgaactc
    39541 gcggtgcagt ggacggcgat ccggccttct gcgctgccac gccgcttcgc ccagcgagct
    39601 gatgacacta cgcagttcgt cagccgaggt gtccgatggt agcggcgagc cgaatgagac
    39661 ggtcactggg taagggatgc gatccggcag tttccagaga aagcggccgc ggttgaagct
    39721 gaagatgctg ccccaaacgc ggtccagatg gaccgggaca atcggcgcag tcttgtcttt
    39781 catgatgcgc tcgaagccgc ggcggaaggg caacagtgtg cctgtccgag tgatctgacc
    39841 ttccggaaag atgcagacaa tctcgccccg gtcgatcgcc gcaccggcgt cacgaagggc
    39901 ccgcaggacc actcgcaggc cgccgtgttg ggaaatcggg atcacgccga gcatcttcat
    39961 gaaaggtttg aagagccgct gctccgcgta ttcggcatcg accacgaagc gcacgggccg
    40021 gtcgagactg gcaatgagta agaagccatc gatgaatgac acgtgattgg gaacgagtaa
    40081 ggccccaccc gtcgtcggca catgctcatg cccgaaaatc cggaggcgat agagactgtt
    40141 ggtcaggatc accagcacga cgcggagcag ggcttcgggc agtagccaga gcgcccacgc
    40201 cgtgccaagg atggtgaatg cggcggtggc cagaaagatt ccgatcgtcg ccaggccgct
    40261 ctgtgccaaa gctccggcgc cgagcgatcc cagaagtatg ccggtaaaga cgcaggtgtt
    40321 ctcaaacgcg atcaccgatc cgcgacggtc agccggtgcg cgccattgaa tgagcgcgtt
    40381 cagcggaacg aagatcagcg cgctggacgt gcccaggacc accatcagcg ccatggttgc
    40441 ggcgaagccg ggggcaaaga ggccaaggct tgtgaggatg atggcgacac cagcggcacc
    40501 gagtggaatc agcccgtact ccacgcggga ttgggacagg cgtccgacga ggatggcacc
    40561 gagaccgatg ccgatcgaga gcatcatgag gggcagcccc gactgccagt ccgacacacc
    40621 cagtacggct ttggcgtata tcagaatgtt ttgggcaaag aggctggcga tcgtccagaa
    40681 gaagacggcg ccggtgatgg cgagccggag catccgatcg gcagccagcg ccgaccaggc
    40741 tccctgcaac gtattgacga ctccgccttc ggctcgtgcc ggtgccacgc ggggaatcgt
    40801 ccaggccgaa cggaatccga cgagcgagaa tcccaccaac aacaatggtg ccagccaggt
    40861 ggaatctccg gtgagggaca agagaatccc cggtgctgca gtgccggtga ggatggcgag
    40921 gaatgtccac atctccagtt gaccgttgcc tgcggccagc cgttcgtgcg ggagcagttc
    40981 ggccagaatc ccgtacttgg ctggactgaa cagcgcgctg tgcacgccca tgccgcccag
    41041 cacgatcagc ggcaggatgc cgccggctgg attgatccag agcgctgcag tggccgctgc
    41101 catcagtagg acttccaccg atttcatcga gatgatcacg gtgcgcttgc tgatgcggtc
    41161 ggccagtacg ccggccacga tcgagacgag cgcaaggggg agggtgaaca cgacgaacgc
    41221 gagggtggtc tgcgtctgcg aggcggtttc gaagtcagga cccggcgcca ttgtcgaagc
    41281 cagttgccgg atgcccagca acgccaccat gagcttccag gcgttgtcgt tgaatgctcc
    41341 gcaaaactga gcgatcaaca ggccgcggag gggatatcgg gagtgcttcg tcatatcagt
    41401 gtcatcacaa cattttgtga ccccgtcctc acccgaggcc gtcggaaggg agtgcaacat
    41461 ctagcataga tagcccgggc tctcgatcgt cagcggcagc cgccagcgtg atcttcagga
    41521 tgtacaggtt cttattgcgc cgttggcgga attcacggac gagttcgaga gccaagtccg
    41581 gtttccgctt gagggtggcg aaggacgtat tgtccatgac ggccaacacg tggccggctt
    41641 cacgaacttt ggacatcagc atgtcctctg atgccaggcg cgggagcgcc ggttccagat
    41701 tcaagtaata caccacgtct tcgttatccg gccatcgagg actgaagaga tggagcggct
    41761 ccgatggatt gtgcggaaaa gatttaattg tatcactggc gcgccgtgct gaagtggcat
    41821 cgtcaatgcc tggcaagatc aatccgacga cagcgattga aaaggacaca gccagtgcgc
    41881 caacggtgcc gagcgccagc ttgatcgtag agcggatgac tgcataggcg agcagcgtgg
    41941 ccagagagag aacgatcatg acaaaagccg gcggaaacac ggacaacgaa gtctcccagc
    42001 gtttctgcaa caggcccgga ccgtaaaaca tcgcgatgac gaatgcgatg gcgagcagac
    42061 ccagcatggc tctcagcaac attgtcgcga gccctttgga tgctggggaa cagtacaggg
    42121 attggcaata acgcccgatc atgaggccga ggggaggaat caggggcatc aaataggact
    42181 cgcgcttgag ggtggacagg ctgagcgccg tgaataatcc caggaaccag aggaggacga
    42241 agagttccga ccgttccagg cgaagtggat gcctggacat gaaccatatc aggctggacg
    42301 gaagcaggac gatccacggg aaaaaatcga accagattat caagagatac caatacatgg
    42361 gatgtcccgg tgtggccgtg tgagatgaga aggcaaacgc ggatttcaag cctttgaagg
    42421 gattgaaatg caagaccagg tcctcgctca acaaactcgc gtaggcagct atgagggcga
    42481 acaggagcag gacggtcgtg tggacagagc gatttttgag cggagagcta tcacgggtca
    42541 atgccagaaa cccgatgatt acgacaagcg gaagggctgc tccgtgcaga cccttggtca
    42601 tcaccgcaaa cgccatcccg actccgacaa ccggatacca ccagcgtccg gcattctgaa
    42661 gatgagcttt ggcccaggcg aagagcgcca aggtgatgaa aaacgtttgc atggaatcga
    42721 aaagcgctcg ccgtccgtac cagagcatga gatgcgaagt ggctacaaca agggctgccc
    42781 agaaccctgc cgtgcgtgag aacaaagatt tgcccaggaa gtaggtgagc atcatcgtgc
    42841 cgaggctgaa caatgctccg ggaagacgaa gggcaatctc gttttcaccg aaccatcttg
    42901 tgaacagagc gagtatccag aaaaacagcg gtggtttgtt gacgtatggt tcatcgtgat
    42961 aggtcaactg aaaccacttc cctgaaagtt gcatctccct gctaatgtgc gcgtacagac
    43021 cttcgctacc ctcaaagaga gcaatatcca ggttgtaaaa gaacgaaaag ctgccaacga
    43081 gcagaagcgc caggctgctc gcgaaattct ctgaaacgga gcatccggtc gcaatgtggc
    43141 tgagggagtt ccgccaagtt acactcaagt tgttcctcca aacttgcacg gtgatatgtt
    43201 ggtcccaatc gtcttttcaa tacgacgcgt tctccgcatg acgctcatca ccgccgctgt
    43261 ccgcctgatc gctgggctcc tccggccgga tgggtgaggc ggtggtaccc cttggtgtcc
    43321 ttttctgtga aggctacggt acgtcgtcag gactgccccg ctgttttttc gctcgataca
    43381 gggagtcctt ccagttgcat gatccgcaat gcgttcgtcg tcggacaggg acccggccgc
    43441 agcttgtagt cgaattcgag tgcgccgcca ctcacggttt cctggaaatg cgcattcgtc
    43501 agcgagtcga tttctttctc caagtcggtc aattccaagt cgtgggtgct gaccagcccg
    43561 agcccgtttc ccgtcgagag ggccttgatg taagcccggc tgccgattaa ccgttcgcgg
    43621 ttattcgtgc ctttgaagat ttcatcgatg agaaaaaaca caggcgatgc cgcacgatct
    43681 tccgttgcgt cgagaatcga cttcagccgc ttcacttcgg cgtaaaagaa cgacatgccg
    43741 gcatccagag agtcgttgac gcggatgcag caggcaagac ggctccatcc ccattgaaag
    43801 gtcgtcgcgc agaccggcgc accggcttga gccaggcaga tgttgatgcc aatggtccgc
    43861 agaaatgtac tcttacccga catgttcgag ccggtgatga ggtgaatcgt gcctggcttg
    43921 gagaggctga tgtcgttggc tacgcgagtg tcctgctgga tcagcggatg gccgagttga
    43981 gtggcgatca gtgtcatgtg ttgacccgga acgggccaga cgtaagaggg atgcaaatag
    44041 gcaaaggtgc cgagtgcaca ggctgcttca acctctgcga gccgatcgag ccacgtaggc
    44101 atctgtttct ggatagcctg ctgaatctgt tggaggcgct ttgtccacca caagtcccag
    44161 ggacagagga cgttaacggc tagatggaca aggggatggg ccttgatgct gatcgcatgg
    44221 agggttcgcg cagcttgggc gacatgggcc gaaggcctgg cccctggtac gaggagtggc
    44281 gcgcaggctg tggccaaggc cgagcctcgc cgcttggcgt ggcgctctaa ataaccgaga
    44341 acctttccga gacgctccat ttcatgatga aggccgatgg catgttcgag gagctcttct
    44401 ccttggtccg tttggagata ggtgagcgca taggccgcga acgaaaacat ccagtagccc
    44461 ggcagccaat tcaacagagt gccaaggccc aggacgatgg tggtgagcgc caagagactt
    44521 tggatggtga gaatcaggtt cagccgcggg aatcccacgg catggtccag aaccgcggca
    44581 agacggcggc cgttgatttc ctgttcgccg gccagatgcg cttccagttc gaggcgatcg
    44641 cgaaacaatg accgcggagt caattctttc accaatcgct ggcgggcttc ccataccggc
    44701 tgaattggcg gctgggtcat gagccagcca ctcaatcgaa gccgcccctg gtcggacacc
    44761 gtactgtcga ggagatgcat cagggaatga gagccggcaa gatcgagatc ctgcgcgtag
    44821 aggtgtgagg cctgtgtctc cgaaggtcgt gtcgggatgt tgggccagtc cagccgcacg
    44881 cgggcaaggt gagcagactt gatgcctgtc cacatgcgca gccgatgaat ccgttgttcc
    44941 agcctggtat gatacgcggc cacgatgagg aagagggtca caaagccggc caggacgaga
    45001 ttgccggtgc tgtaccagcc ggatttgtag agcgtgacga cgcaaagaag gccggcgatg
    45061 aagatcgcca agcgccattt ggtgaaccgg gagctggcgc tcgttccttg atcgatgaga
    45121 cgaccgagtc ttgcgatcag gcgttggagt tgggctgtgc gggaaaatgg agccatcgtt
    45181 taggggacct tactggaagc ctcgaatgtc gtcaacgatt tctgacagac tgttgaaaaa
    45241 acttgccagc ttcgttctcg ctacgcgtag agggtcaccg tacgacgaga agtatgtttc
    45301 gcttcctcgc ttgctgcggc cttgaacgtg gagcggcgcg tttcggcgcg ccggggttgg
    45361 gtgggtgaga ccagcagctt tttgaacagt ctagggggac ggtctatggc gatgtttgtt
    45421 tgtagagcaa ggcatgtcct ctgactggat agacgttcag atttactcct cggtcgatgc
    45481 gggggaggtg ctgggattga tcgacgatcc cgccgtgcag ggcgcctggc aggacggagg
    45541 aatgatccat ctctattggc ccagccggaa ctggtcgccg gatcatcttg tccg
//
LOCUS       DATIFR010000112        21802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1414015, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000112 DATIFR010000000
VERSION     DATIFR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21802
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414015"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VLD60_06220"
     CDS             <1..355
                     /locus_tag="VLD60_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB44587.1"
                     /translation="EAYKDYPGITSGWILFFAGCLWQLDPTFFNVLLHDFESFTKGYD
                     YKQILAKIDCPVLFLWGEPKLGAVMTDDEISWLRQNFSNARFAPIDGVGHLLHLQDQG
                     QTPVLNAMTAFLDRI"
     gene            1089..2105
                     /locus_tag="VLD60_06225"
     CDS             1089..2105
                     /locus_tag="VLD60_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44588.1"
                     /translation="MLRDKLLIVRNFMHGTAGILYHIVVVALSAGIALSLPTVARSFL
                     TQWSRVENDKVYLIAVEVTMAIFLIVSINYLHRSFRDRILANTATSAGLTAFFSWRKL
                     SAQKRIRKLKEKQGTGRTVMLIGSTGYGTFVDQEGDLHTVLKKCLGAHILLVNPYSQQ
                     ATSRIDAVLHPHFTLEKFRTEVRESITLLKRLKATGKAVKLKLYSDPPLVKLTILGDH
                     LWLKHYHTDLDVQTMPQYLFQHNLNDHGFYSLFYQYFMQRWDNPEIPEYDFDTDELVY
                     RGHNGNVILRERFETGKESEDNRQALGSSPDIFPVPGRLGRAAVAMPSRNQTSEPRTT
                     LPHL"
     gene            complement(2152..2595)
                     /locus_tag="VLD60_06230"
     CDS             complement(2152..2595)
                     /locus_tag="VLD60_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSB44589.1"
                     /translation="MASYLSRSCARVCVVTLLLATVAAHAGDKSSQPSTAQQQRIEIV
                     IQDKTFFLANAVPIQVGQSIEIIVRNEDQVRHGFASASLSGLLVSGEDDQIVMYGKGV
                     EGFYVNPGKTLIIRIDAERPGSMPFHCDLHEHMKGELYVLEVPTA"
     gene            3292..5013
                     /locus_tag="VLD60_06235"
     CDS             3292..5013
                     /locus_tag="VLD60_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HSB44590.1"
                     /translation="MLRTYGPVSCLNLTLSTATFLLDMVDASCPATFPWWQSAVFYQI
                     YPWSFQDSNGDGVGDLRGIRSRLDYLRDGTSASLGVDAIWLSPIYSSPMRDFGYDVAD
                     YCNIDPRFGALTDFDELLRDAHRRGIRVIMDLVLNHTSDQHRWFLESRASRHSPTGNW
                     YCWADSKTFGRRPSNWNARFGGSSWTWDPSRRQYYLHSFLKEQPDLNWQHPSVRAAMW
                     DVVRFWLDRGVDGFRLDAINWLGKDMRWPNNPFRFGLRGYTRQHHLYDRDQPMVHQIM
                     QELRHVVSAYPDVVLIGEAAADTAGGPASFYGHGNDELHLVFDFRLMKSPWQADRFRR
                     FLSEHVPAIPKGGWPSIVFSNHDQPRHIDRYGAGGDAQARARAAALLLLSLPGTPFLY
                     YGEELGMRNGKLRYRDLRDPYTKHFWPFRPGRDPARTPMQWDTTCQAGFTSGRPWLPV
                     AQDYPQLNVAREASDPRSLWCLYRRLLELRRISPALSRGECRLIEGSPRDCLLYQREA
                     RTEEGTIDRMLVAVNFVERACEFTLPAGSSHGRVLISTDPDAGNALWNPGRIQLGPNE
                     GLLVRLS"
     gene            complement(5176..7107)
                     /gene="glgB"
                     /locus_tag="VLD60_06240"
     CDS             complement(5176..7107)
                     /gene="glgB"
                     /locus_tag="VLD60_06240"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSB44591.1"
                     /translation="MPKTPSATKKADDMSLFSKDDLYLFNEGTHFHLYDKLGAHPARK
                     DGTSGTYFSVWAPNAEEVSVTGLFNDWKPGVHPLHQREQSGIWEGFLPSVEKGALYKY
                     HIRSRFDVYQAEKTDPFAIFNEIPPKTASIVWDCEYDWRDDEWRKMRRSRNALDAPIS
                     IYEMHLGSWKRVPDEDNRSWSYRELAAVLPQYLQDLNFTHVEFLPIMDHPFFGSWGYQ
                     TTGYFAPSGYYGTPQDLMYLIDELHRHGIGVILDWVPSHFPTDGHGLSYFDGTHLYEH
                     ADPRQGYHPDWQTYIFNYGRNEVRSFLISSALFWLDKYHVDGLRVDAVASMLCLDYSR
                     KEGEWIPNQYGGRENLEAIGFLRRLNEEVYKRFPDVQTIAEESTAWPMVSRPTYLGGL
                     GFGLKWDMGWMHDTLDYMSQDPYFRRYHHNKLTFRMLYAFHENFVLPLSHDEVVYGKG
                     SLLGKMPGDEWQKFANLRLLFGYMYAQAAKKLLFMGSEIGQWSEWAHDRTIEWHLLEY
                     ASHRGLERWVADLNRLYRDEPALHERDLDPAGFEWIDCNDAESSVISLLRKGKSADEI
                     MLAACNFTPVPRPNYRIGAPCGGFWKEVLNSDATCYGGGGWGNMGGVEAVPISLHGRS
                     HSLTLTLPPLAAMFFKWVR"
     gene            complement(7206..8855)
                     /locus_tag="VLD60_06245"
     CDS             complement(7206..8855)
                     /locus_tag="VLD60_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HSB44592.1"
                     /translation="MLTASQDWLHLFERETVECLIRMLPSVLRTRRWFGGKGRRIEAA
                     RMVESIAIPSGSTTTMLLLIQVEYGDGGFETYTLPVTAAFGEEEERIQQDFPQAVIAP
                     VAVQRNDREETGLLYDALWNRDAALALLQAISQESQFKGTAGSLNASSTNAFADLISA
                     GTLPEPAVMKAEQSNTSVAYDNHVILKLYRRLERGMNPDLEIGRVLTRMQFPYTPPIA
                     GALEYHCGTGEPVTLALLQKFVSNDGDAWRYSLEAVNQFLVRIIGEHLRDEPPPQTGF
                     PPLELARQEYSPVARQLIGPYLESAERLGRQTAELHVALSQVVNDPAFAPEPFTKEYR
                     QARYDYMVRSTAGTLALLEERMEVLSTVGQTQARRLLNLKPALERTFHAFRDLESPIS
                     RIRCHGDYHLGQVLCTGADFVITDFEGEPARSLAERRMKHPAMVDVAGMARSFHYAPF
                     AFFEGKRAGIVTASQENPRHSALWARFWSDWANAAFLKGYLNIATGARFWPRDDEDIR
                     LLFDAFLVEKAMYELGYELNNRPDWAEIPLHGLVEILEIKG"
     gene            complement(8887..10881)
                     /locus_tag="VLD60_06250"
     CDS             complement(8887..10881)
                     /locus_tag="VLD60_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HSB44593.1"
                     /translation="MIDEYGRQRVVVERVRPEIDGGRFPIKRTVGEDVVVEADVFADG
                     HDALSVLLQYRHEADQQWMDVPMEFLGNDRWRASFPVTTVGRYRYTVTGWVDHFVTWR
                     RDLIKKIDGGQDVQVDVLIGAKLIDEAVGRATKEDGRKLAIWVTELRSAATPESHRIR
                     VTLSPEVATLMAKYPDRRFATMYDRELAVVVDREKARFSAWYEMFPRSCSPKAGQHGT
                     FKDCEARLPYIASMAFDVLYLPPIHPIGRTHRKGKNNAPTCGAADPGSPWAIGALEGG
                     HTSVHPQLGTLQDFQQLMQQARSYGIEIAMDIAFQCSPDHPYVKEHREWFRIRPDGTV
                     QYAENPPKKYQDIFPLEFETDQWRELWDELKNVIDYWIGEGVRIFRVDNPHTKPFALW
                     EWLIREVKQTHPDVLFLSEAFTRPKVMYRLAKLGFTQSYNYFPWRNTKSQLMQYFTEL
                     TQTEVREFLRPSLWPNTPDILTEHLQHGGRPAFMSRLVLAATLGASYGIYGPAFELQE
                     NLPREAGGEEYLNSEKYELKQWDLDRPDSLREFIGCVNRIRRENPALQSDWSLCFHEI
                     ANESLVSYSKRTSDGSNIILVVVNLSPHHVHSGWLELDLGSLGLDAEHPFQMHDLLTN
                     AHYLWQGPRNYIEVDPLSAPAHIFRIRRRIHTERDFDYYL"
     gene            complement(10926..11624)
                     /locus_tag="VLD60_06255"
     CDS             complement(10926..11624)
                     /locus_tag="VLD60_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44594.1"
                     /translation="MSEMWQEAMIEGFRETMKRIGLFLPKLTALLTFLVLGLAIGWLV
                     KVLLLRTLKTFRFDQLCERLGVTPALGKAGMKQPVSHMIGRLSFWIVFLLFTFMGIDA
                     LDLPATANLMNVILGFLPQVLAAILVALIGALLANFSGEATLIAAVNAQIEEAQLIAN
                     LIRWGVLLFTAAMVLTQLGIAKEIVVAAFSITFGGIVLALAIALGLGGRTIAKEALER
                     RLRRRTTVDEVSHI"
     gene            complement(11621..12901)
                     /locus_tag="VLD60_06260"
     CDS             complement(11621..12901)
                     /locus_tag="VLD60_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HSB44595.1"
                     /translation="MTQVTTWEQVREQCPDVSKVDLLVGVPTFDHASTVEPVMKAIVA
                     GLEASFSHASILLVNADAGSQDGTPEMVKGAVGGPLPVALVQHVTGSMFSNPFAHLRL
                     SESGVPAREHAFRAFFTIAEELQAKACIVIDANLRSVTPEWIERLARPVLEKEADYVA
                     PLFQRQRYEGSLTNSLIAPLTRALYGKRIACQTGGGYGFSGKLASLYLQRDVWEGDAA
                     RFGIDSWLTTVAVAEGSNVWQAFLGTKVQDTKASGLDVSTVLAQAVGASYHYMERYQD
                     VWEKQMGSSPVPTSGPSYELGSESVVINVERMVKGFRQGLRDLLPIWEMILAPETLAG
                     ILPLGLVDEDGFRFPIPLWVQIIYDFALAYRDKVIHREHLLKSLTPLYLGRTASLVLE
                     TKDGGPDEIERTIEQVCEWFELLKPYLVGRWRFQ"
     gene            complement(12969..14168)
                     /locus_tag="VLD60_06265"
     CDS             complement(12969..14168)
                     /locus_tag="VLD60_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB44596.1"
                     /translation="MAQELDEYRDYAPKGTVEFLYRLSDLVAGRSFLHVNAVRYGGGM
                     AEILRRLVPMMKALGVDARWEVLAGDQEFFHISKQLASALQGQEETLTEQLEQIYLKI
                     TQRNAQSLNLDADLVMIHDPPPVALIEHRKGGTWIWRCYLDLARPQQRSWSFLRRFVV
                     RYDAAIFSLPGFAHRLPITKFLIYPSIDPLSEKNRELSRVEINQVLVRLGIPRDKPIL
                     LQLARYERFKDPVGAIKVYRLIKKHHDCRLILAGAGVTHDPEGEMVLAEVLEAAGGDP
                     DIHVLQLPPEADFEINALQRAATIVLQKSIKEGFGVAVSEAMWKGKPVIGSKAGGIAA
                     QIVDGVTGYTVHSVEGAAFRGRYLLNNPGVIQRMGGAAREHVRRNFLVTRHLIDYLTL
                     VKLFANR"
     gene            complement(14201..15880)
                     /gene="treS"
                     /locus_tag="VLD60_06270"
     CDS             complement(14201..15880)
                     /gene="treS"
                     /locus_tag="VLD60_06270"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HSB44597.1"
                     /translation="MPRADPLWFKDAVFYELHVKAFYDGNDDGIGDFLGLIQKLDYLE
                     WLGVDCLWLLPFFPSPLRDDGYDVADYRGIFPDYGSMEDFRTFLNEAHRRGMRVIADL
                     VLNHTSDQHPWFLESRSSPQSPKRDYYVWSDSDKKYGNARIIFIDTEKSNWTWDPVAK
                     AYYWHRFFSHQPDLNYDNPELQKEMLDVMSFWLDLGLDGFRCDAVPYLFEREGTICEN
                     LPETHAYLKEVRKRIDQTYQGRILLAEANQWPADVRPYFGDGDEFHMAFHFPLMPRLF
                     MGVGSEDWHPIEDMFTHTPPIPENCQWCLFLRNHDELTLEMCSGEERDYMYYAYARDP
                     QMRRNIGIARRLAPLLDNDRRKIELLNSLVFTLPGSPIVYYGDEIGMGDNVHLGDRNG
                     VRTPMQWSADRNGGFSKADPSKLYLPVTSDSVYGYQATNVEAQRQSPHSLLHWMKQMI
                     SVRKKHPAFRRGTLAFLHPRTDNVLAYLREYRGDTLLLVHNLARSAQAVELDLAKFEG
                     AVPIELFGGSRFPTVGKHPYAFSLAPYGFYWFKLQQPLSPDGSYGIEGSII"
     gene            complement(15898..16566)
                     /locus_tag="VLD60_06275"
     CDS             complement(15898..16566)
                     /locus_tag="VLD60_06275"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HSB44598.1"
                     /translation="MRGTDRPFRFTGCSELREILGEEAEDEKRLVELLEAVPLDSIYF
                     HTHSYFLRHSYVERVYPNDFAQWVAMEVRDHVLGERLAVVDLFEFEGLDALRAELISI
                     IDDHLSRTPIVPRVIFGEPFYFNQSRILAVPTGLEVRTLREFRHALGEVEVSAIYFHI
                     FEARHRLKGEESDFSEWIRQSLGMPELAEKLRAINPYLGSLERLRSALLTVCDEFLAR
                     EPMR"
     gene            complement(16809..16964)
                     /locus_tag="VLD60_06280"
     CDS             complement(16809..16964)
                     /locus_tag="VLD60_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44599.1"
                     /translation="MKSIAFSGVLELNVYNATVEEKMSAEPHSNPVEERFIRSGTSLC
                     VGTFVEQ"
     gene            complement(16995..18413)
                     /locus_tag="VLD60_06285"
     CDS             complement(16995..18413)
                     /locus_tag="VLD60_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44600.1"
                     /translation="MGLGQDQRTASQPTILELGSGGTGSRVERTRWLLTRDFSLIWWN
                     QVLSQVADGVSRLALLWFVYSVTGSPLKTSVIGLLQTLPPILLGPLIGVSVDRLPKKA
                     ILIGSDVARAILIGLIPCWVSTDMFTVESLYVLVFLYGIATAMFVPALSASVPFLVGR
                     SRFTAANALLQSTTSIGMIMGPVLSGLGIAFSGSQEVLCANAVTYVAAALCLVPIQLP
                     RPMMAHPRSPVAATRGDLTEGIRYALRSQPTIQVLILMASVYTFGTSALTTLFPVFGK
                     KMLALGPVEVGYLWAWLGIGMFLVSLGLVSVTQWEVRKRLQVVSVSSVIGGAALCGLT
                     WTQDLMMATVLMASIGMGFGTWTPIAWGMIQEVSPAHMVGRVMAIYTAIATATSMAGI
                     AFFGWLTERFNEDTSVIGIGFVLFLLALVAAWFSRRVTDTGDPADVTEREARTIVRTF
                     ADEPLTREGNLCFASPCPPRTG"
     gene            complement(18574..19842)
                     /locus_tag="VLD60_06290"
     CDS             complement(18574..19842)
                     /locus_tag="VLD60_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44601.1"
                     /translation="MQSESADQDNRGWRLVKTWDFGCLWWGQVTSQVGEALSKVALLW
                     FVYDLTGSALMIALVGLLETIPPLVFGPLIGVYLDRLPKKTVMIWVDLTRSVMTFLIP
                     ALYGLGLLSLEGLYILIFLTATVSTVFGPALVSAVPLLVRRCELVSANALIQGTNNIG
                     ILLGPAISGVLIALIGAQHALYVNSATFFISALCLLPIRFNETRWRTQEISARSGAVL
                     SDLTVGFRFVFGDRSTVFLLVIIAALYNLGASAFVFVLPVYAKELLHAEPVQLGWLWS
                     SLGVGMLIASSWLAWRRKDDVQSRMHLLIRGTTVGGLAVCSLSLLESPLIAAAVVILV
                     GASGAVLNPVVWALLQEATPSHLMGRVLTTFSTGSMAAAMIGMTGFGWAADAIGPAAS
                     LVGLGFVLLLTAGVATLFARQAMSMEVAVT"
     gene            complement(21535..>21802)
                     /locus_tag="VLD60_06295"
     CDS             complement(21535..>21802)
                     /locus_tag="VLD60_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HSB44602.1"
                     /translation="FYFPREVRIRTGEKVVWFYADGSRAPHTVTSGACRGADCSGGGQ
                     AFASGPTLLKPGDRFEHTFTRPGTYPYHCEFHTATMQATVIVSP"
BASE COUNT         4801 a         6456 c         5850 g         4695 t
ORIGIN      
        1 cgaagcatac aaagattatc ccggcataac cagcggatgg atcttattct tcgccggctg
       61 tctgtggcag ctcgatccca ccttcttcaa tgtgctgcta cacgatttcg agagcttcac
      121 taaagggtat gactacaaac aaatcttggc gaagatcgat tgtcctgttc tgttcctatg
      181 gggggaacct aagctcggcg cggtgatgac agatgacgaa atttcctggc tgcgacagaa
      241 tttcagcaat gcgcgattcg ccccgatcga cggagttggt catcttctgc atctccagga
      301 ccaaggtcag acaccggtgc tgaatgcaat gacggcattt ctcgatcgca tttagcttgt
      361 tcctgtacga gtgcttggcc acatcagcct ctcccataga cacaagacct taaatgagag
      421 tgcgatctgt atggtgcaga atccttgcgt ccaaatcaga aataactgat gaaatatgac
      481 ggactttcat ccatcacatc aggtgatcaa taagaactgt tttggagtgc gtcatgcggt
      541 catgtttttg tcgtgcactg ggtagatatt cgctgattct cgcagttggg tttcttgggt
      601 ggcgaacaca tcaagacctc aaaatttttc acccttctaa ctctttaaca acacagagag
      661 tcgctttaac aacacagaga gtcgatcaac ggagtacgat cacgcgtatg gccttcttgc
      721 ttgacgctca gtcccctgcg ttgtgccctc tggagaccga ggtcaagcct agatgaataa
      781 caatccatcg tgccaggcga gattaggtgc gttgacatcg ctgcatgtgc agcctatggt
      841 gtagatcttg cagttagaac tgatcattgc ttaagcgctc gctaatattc tcacagcgaa
      901 tcgcgtagct gccccctaaa ttagccggga accgaccaac tcctgacttt gaaaccggca
      961 ggagtcgcag cgggtttatt tttatagaac cgatcgaaca tcttgctggg aaatggggca
     1021 acgcacgatc ggcccagctg aggaaccatg gtgtttattg tattgggaac acgggaagag
     1081 gtgactcgat gttgagagac aaactgttaa ttgtccgaaa tttcatgcat ggcacggcag
     1141 ggattctcta ccacatcgtc gtggtggcac tgagtgcagg gatagccctg tctctaccca
     1201 cagttgcgag gagtttcctt actcagtggt ctcgggtgga gaatgataaa gtctatctca
     1261 tcgccgtgga agtgacgatg gcgatttttc tgatcgtctc cattaactat cttcatagaa
     1321 gctttcggga ccggattctc gctaacacgg caactagtgc tggcttgacc gcttttttct
     1381 cttggcgaaa actcagcgct cagaagagga tcagaaagtt aaaggaaaag cagggaacgg
     1441 gaagaactgt catgttgatt gggtcaactg gctatggaac ctttgttgac caagagggtg
     1501 atctccatac ggtactgaaa aagtgtctgg gagcccacat actgcttgtg aatccgtata
     1561 gccagcaagc gacaagtcgc atcgatgcgg tcctccatcc ccattttact cttgagaagt
     1621 ttcgaacgga ggtcagggaa agcatcacat tgctaaagag gctcaaggca actgggaaag
     1681 ccgtcaaact caagctctat tcggatccac cgctcgtcaa actcaccatc ctcggtgatc
     1741 atctttggct aaagcattat cacaccgatc tggacgtgca aacgatgccg caatatctgt
     1801 tccagcacaa ccttaatgac catggcttct atagcttgtt ttaccagtac ttcatgcaga
     1861 gatgggacaa tcctgaaata ccggaatatg atttcgatac tgacgagctg gtgtatagag
     1921 ggcacaatgg caacgtgata ttaagagaac ggtttgaaac ggggaaagag tctgaggaca
     1981 atcggcaagc gttggggtct tcccccgata tattcccggt accaggacgg ttaggtcgag
     2041 ctgccgtggc gatgcccagc cgcaaccaga caagtgagcc tcgtactact ttgcctcacc
     2101 tgtaaagcga ttgctaatcc atcgcccgta cgaaaatatt tctttttgga actaggcagt
     2161 gggaacctcg agcacgtaca gttcgccctt catgtgttca tgaagatcgc aatggaacgg
     2221 catactgcct ggccgctcgg cgtcgattcg gatgatcaat gtcttgcccg gattcacgta
     2281 gaacccctcc acccctttcc catacatcac aatctggtcg tcttctccag agaccaagag
     2341 gcctgagaga ctcgcggagg caaatccatg gcggacttga tcttcattgc gcacgatgat
     2401 ttcgatggac tgcccgactt ggatggggac cgcgttcgcc aggaagaacg tcttgtcctg
     2461 gatgacgatt tcgatccgct gttgttgtgc cgtcgaaggc tgcgatgact tatctcctgc
     2521 atgcgcagcc acagtggcca acagcagtgt gaccacgcag acacgcgcac agcttcggct
     2581 cagatagctc gccatcttgc acctctctca tgtcggaaag ttcagaacca ttactttttg
     2641 aaccgacgtt agtatggggg aaaatccttg agaaatcgac tagcctcctt ggcattctaa
     2701 acaaggattt tcacaccgat gagtcgcgag ttttcacatt gcctaattct cccctcccca
     2761 acctgtgatc tccggtgtca aggcggcctg atcggtctcg ctgggcccgg acgagacgaa
     2821 acggttcccg acacctttcc tttctaggcc gtaggaacag caattaggaa agcgcgattg
     2881 tgaccctact taggcctact ggatctcact caagccaact ggggccaagg cttttgaaaa
     2941 atgcaaactt tggctgcagt agggctgagt gggctggatt gaggtgggct tcagcacaaa
     3001 tcgagcacaa tcaccacaac ccatggcaat gccccctgat gccgtgtata cgaccttaag
     3061 cgggttagct cctgatccta gctgctgaac ttttactgga gcaaatcaat ccatcggcca
     3121 tcaatgccct cttcgccctc ttcggtcggc cacgcgacca ctgcgcggcg actcccattg
     3181 ctcgcaagtt ccatcgagtc cccgagaccc ttaaagatcc agtgagaatt atttgccagc
     3241 ctcaacgtca caggcgggga ccacacattg ctggtttcat tggcttcagt gatgttgaga
     3301 acgtatggcc ccgtttcctg cttgaatttg accctgtcaa ccgctacgtt tctgctcgat
     3361 atggttgatg catcctgccc tgcaacgttc ccctggtggc aatccgcggt cttttatcaa
     3421 atatatcctt ggagttttca ggactcaaat ggcgacggcg ttggtgatct gcgtggcatc
     3481 cggtccaggc tggattatct ccgggatggg acttccgctt cactcggagt ggatgcgatc
     3541 tggctgtctc ccatctattc ctctcctatg cgggatttcg gctatgacgt ggctgattat
     3601 tgcaacatcg acccgcggtt cggagcgctg accgacttcg acgagctcct ccgcgacgcg
     3661 caccgtcgcg ggatccgcgt gatcatggat ctcgtcctta accacacctc cgatcagcat
     3721 agatggtttc tggaatcacg cgcctcacgc cactcgccca caggtaactg gtactgctgg
     3781 gcggacagca agacgtttgg gcgtcgaccg agcaactgga acgcccgctt cggcggatcc
     3841 tcctggacct gggatcccag ccgccggcag tactatctcc actctttttt gaaagaacaa
     3901 ccggatctga actggcagca tccctccgtg cgggccgcca tgtgggacgt cgtccgcttc
     3961 tggctcgacc gaggtgtcga tggctttcgc cttgacgcca tcaattggct gggcaaagac
     4021 atgcgatggc cgaacaaccc gtttcgcttc gggttgcgtg gctataccag gcagcatcac
     4081 ctctacgacc gggatcagcc catggtgcat cagatcatgc aggagttgcg tcatgtcgtc
     4141 tcggcctatc ctgacgtcgt cttgattggc gaagcggcgg ccgacacggc gggcggaccc
     4201 gcctcgtttt acgggcatgg caacgacgag ctacatctgg tgttcgattt tcgtttgatg
     4261 aagtcaccct ggcaggccga ccgctttcgt cggttcctgt cagaacacgt gcccgccatt
     4321 ccgaaaggag ggtggccatc gatcgtgttc agcaatcacg atcagccgcg tcacatcgac
     4381 cggtatggtg ccggcgggga tgcccaggcc cgagcgagag cggccgcact gttattgctc
     4441 agcctgcccg ggaccccatt tttatattac ggcgaagagc tcggcatgcg gaatggaaag
     4501 ctccgatacc gggacttgcg cgatccctat accaagcact tctggccctt ccgccccgga
     4561 cgcgatccgg cgcgcacacc gatgcaatgg gataccacgt gccaagccgg gttcacgtcc
     4621 ggccggccct ggctccccgt cgcgcaggac tatccgcaac tcaacgtcgc tcgggaggcg
     4681 agtgatccgc gctcgctctg gtgtttgtac cggcggctac tcgagctccg ccgaatatct
     4741 ccggcgttga gtcgaggaga atgccggctt atagaaggaa gcccccgtga ctgtctcctc
     4801 taccagcggg aggcgcgcac tgaggagggc acaatcgaca ggatgctggt cgccgtcaat
     4861 tttgtggaac gggcgtgtga attcaccttg cccgctggtt cttcacatgg ccgcgtgttg
     4921 atctcgacgg atcccgatgc cggtaacgcc ttgtggaatc ccggtcgtat ccagctgggt
     4981 cccaacgagg gtcttctagt gcgcctgtct taacgagcgc cgctatagtc tcgacatcgc
     5041 gagggactat ttggcatgca attgcacgcg ttgcagttga tcaatcacga tacgagagaa
     5101 aagcagaggg ccacccgctg gttttagcat cgattcccta tcgtacccga gtctaaggcc
     5161 gtccgctcac ggtgctcacc gtacccattt gaaaaacatt gcggcaagcg gtggcagtgt
     5221 cagggtaaga gaatgtgagc ggccgtgcag ggaaatagga accgcttcta ccccgcccat
     5281 attcccccag cccccgccgc catagcaggt cgcgtcgctg ttcaggactt ctttccagaa
     5341 tccaccgcac ggagcgccga tgcggtagtt cggtcgtggt acgggcgtga aattgcaagc
     5401 ggcgagcatg atctcgtcag cagatttccc tttgcgcagc aggctgatca cactcgactc
     5461 ggcatcatta caatcgatcc attcgaagcc ggcggggtcc aggtcgcgtt cgtggagagc
     5521 cggttcatca cgatacagcc gattcaagtc cgccacccaa cgctcgagcc cgcggtgcga
     5581 agcatattcc aacaaatgcc actctatagt acggtcgtgc gcccattcgc tccattggcc
     5641 gatttcactg cccatgaaaa gcaacttctt tgcagcttgc gcatacatgt atccgaacag
     5701 gagccggagg ttggcaaact tttgccactc gtcgccgggc atctttccga gcaaagatcc
     5761 tttcccgtaa acaacttcat catgcgagag cggcaggaca aagttttcgt ggaatgcata
     5821 gagcattcgg aacgtgagct tgttgtgatg gtaccttcgg aaatagggat cctggctcat
     5881 atagtccagc gtatcatgca tccatcccat gtcccatttg agaccgaaac caagtccgcc
     5941 gaggtaggtt ggcctcgaga ccatcggcca agcggtggac tcctcggcaa tcgtttgcac
     6001 gtccggaaag cgcttgtaga cctcctcgtt caaccggcgt aagaacccaa tggcctcgag
     6061 gttttcgcgt cctccgtact ggttcgggat ccactcccct tccttgcgcg agtaatccaa
     6121 acacagcatc gacgcgaccg cgtcaacgcg gagtccatcg acgtggtact tgtccagcca
     6181 gaacagggcg ctgctgatga gaaaacttcg tacttcgttt cgcccatagt tgaagatgta
     6241 ggtctgccaa tcgggatggt atccttgtcg cggatcggca tgctcgtaga gatgcgtccc
     6301 atcgaagtaa cttaatccat gtccgtccgt cgggaaatgt gacgggaccc agtcgaggat
     6361 cacgccgatc ccgtgacggt ggagttcgtc gatcaggtac atcaagtcct gcggcgtacc
     6421 gtaatagccg gatggcgcga aataccccgt cgtctgatag ccccaagacc cgaaaaacgg
     6481 atggtccatg attggcaaga attccacgtg tgtaaaattc aggtcttgga ggtactgcgg
     6541 cagcacggcg gccagttctc ggtaactcca cgagcggttg tcttcatccg gcactcgctt
     6601 ccaggaaccg aggtgcatct catagattga aatcggcgcg tcgagcgcat tgcgggaacg
     6661 tcgcatcttc ctccactcgt catctcgcca atcgtactcg cagtcccata cgatcgaagc
     6721 cgtcttaggg ggaatctcgt tgaagatggc aaacggatcc gtcttctcgg cctggtacac
     6781 atcgaagcgc gagcggatgt gatacttgta caacgcgcct ttctccacgc tgggcagaaa
     6841 gccctcccag attcctgatt gctcacgctg gtgcaaggga tgcacgccag gtttccaatc
     6901 gttgaagagg ccggtgacgg aaacttcctc tgcattcggt gcccagacag agaaatacgt
     6961 tccacttgtg ccatccttcc gagcggggtg ggctcctaac ttgtcgtaga ggtggaagtg
     7021 cgtcccttca ttaaagagat acaggtcatc tttgctgaac aaggacatat catcggcctt
     7081 ctttgtcgca ctcggtgttt tcggcatcgt gtcacctcat tcatgcaact gatgcaagct
     7141 gtgacaattc ctcagttcga accagttgaa cactccagct ggctcgcgcc ccatcgcaaa
     7201 gtgccctaac ctttgatttc caaaatctcc acgagaccgt ggagcggaat ctccgcccaa
     7261 tcgggccggt tattcagttc gtaccccagc tcatacatgg ctttctcgac gagaaacgca
     7321 tcgaacaaga ggcgaatatc ctcgtcgtcg cgcggccaga atcgtgcgcc ggtcgcaatg
     7381 ttgagataac ctttcaagaa tgcggcgttg gcccagtcag accagaaacg ggcccacaga
     7441 gcgctgtggc ggggattctc ttgcgaggct gtgacgatgc ctgcccgttt cccttcaaag
     7501 aatgcaaagg gagcatagtg aaatgaccgg gccatcccag cgacatccac catggcggga
     7561 tgcttcatcc ggcgctcggc caatgaacga gccggctctc cctcgaagtc ggtaatcacg
     7621 aaatcggctc cggtgcacaa cacctgcccc aggtgataat cgccgtgaca gcgtatgcgg
     7681 gaaatcggcg attcgaggtc tctgaatgca tggaaagttc gttcgagcgc tggtttgagg
     7741 ttcaagagac ggcgcgcctg tgtctgtccg actgtcgaga gcacctccat gcgctcctcc
     7801 aggagggcta aggttcccgc cgtgctccgc accatgtagt catatcgcgc ctgtcggtat
     7861 tccttcgtga aaggctccgg ggcaaaggca ggatcattga ccacctggct gagtgccacg
     7921 tgaagttcgg ccgtctgtcg gcccaatcgt tcagccgact ccaggtacgg cccaatcaat
     7981 tgacgcgcca cgggagaata ttcctgccgt gccagttcca ggggcggaaa tccggtctgg
     8041 ggaggcggtt cgtcccgaag gtgttctcct atgattcgga ccaaaaattg attcaccgcc
     8101 tcaaggctat accgccatgc gtcgccgtcg ttggaaacga atttttgcag aagcgccagc
     8161 gtgaccggct cgccggtgcc gcagtggtac tcgagtgccc cggcaatcgg tggagtgtac
     8221 gggaattgca tacgggtcag cacgcgccca atttcgagat ccggattcat ccctcgttct
     8281 aaacgccggt acagcttcag aatgacatga ttgtcatagg ccaccgatgt attgctctgc
     8341 tccgccttca ttacagctgg ttccggtaag gttccggctg atatgagatc tgcaaaggca
     8401 ttcgtggatg aggcgttgag agaaccagcg gtccccttga actgcgattc ctgactgatc
     8461 gcctgcagaa gcgcgagtgc ggcgtcgcga ttccagaggg catcgtacaa tagcccggtc
     8521 tcttcccggt cgtttctctg aaccgcaacc ggcgcaatga cggcctgcgg aaaatcctgc
     8581 tggatacgtt cttcctcttc cccgaacgcc gccgttactg gtagcgtgta tgtctcgaaa
     8641 ccgccatctc cgtactcgac ctgaatcaac aggagcatcg tcgtcgtcga gccggacgga
     8701 attgcgatag attcgaccat ccgggccgcc tcgatacgac ggccttttcc cccgaaccag
     8761 cgccgtgtcc gaaggacgct cggcagcatc ctgatcaggc actccacggt ttctctctcg
     8821 aagagatgca accagtcttg gctggcggtc agcatgcctc tgcgctcctt tgaatcgatc
     8881 gagccactag aggtaatagt cgaagtcgcg ttccgtatga attcgccgcc gtatacggaa
     8941 aatatgcgcc ggagccgaaa gagggtcgac ctcaatgtag ttcctgggcc cctgccacaa
     9001 gtaatgcgcg ttcgtcagca gatcgtgcat ttggaaagga tgctccgcat cgagccccaa
     9061 acttccaaga tccaattcca gccaccccga atgaacatgg tggggactca ggttgacgac
     9121 gacgagaata atgttggacc cgtctgacgt gcgcttgcta taagaaacta agctttcgtt
     9181 cgcgatctcg tgaaaacaga gactccagtc gctctggagc gcgggatttt cgcgccggat
     9241 acgattcacg cagccgataa attccctcag actgtccggc cgatcgagat cccactgctt
     9301 gagctcatat ttctccgaat tcaaatattc ctcgcctccc gcctcgcgcg gaagattctc
     9361 ctgcagttcg aatgccggac catagatgcc ataactggct ccgagcgttg ccgctaacac
     9421 caaccgagac atgaatgcag ggcgtccccc atgctgcaga tgctctgtca gaatatccgg
     9481 ggtgttgggc cagaggctcg gtcgcagaaa ttcccgtacc tctgtctgag tcagttctgt
     9541 gaaatactgc atgagctgcg acttcgtatt ccgccacgga aaataattgt aggactgtgt
     9601 gaaaccgagt ttcgccaggc gatacatcac cttcggccgt gtgaatgctt ctgagagaaa
     9661 gaggacatcc gggtgtgttt gtttcacctc cctgatgagc cattcccata atgcgaaggg
     9721 cttcgtgtgc ggattgtcca cgcggaaaat ccgcacaccc tccccgatcc aatagtcgat
     9781 gacgttcttc aattcatccc acagttcacg ccactggtcc gtctcgaact caaggggaaa
     9841 gatgtcttga tatttctttg gaggattctc cgcatactgc accgtgccgt ccggcctgat
     9901 ccggaaccac tcacgatgct ccttcacgta gggatgatct ggcgagcatt gaaacgcgat
     9961 gtccatggcg atctcgatgc catagctgcg tgcctgttgc atcaattgct gaaagtcctg
    10021 gagggttccc aactgaggat gaaccgatgt atgtcctccc tccagagcgc cgatggccca
    10081 agggctgccc ggatccgcgg caccacaggt aggcgcattg ttctttccct tccgatgtgt
    10141 ccgcccaatt ggatgaattg gaggtaaata caatacgtca aatgccatcg acgcaatata
    10201 aggcaggcgt gcttcgcagt ctttgaaggt gccatgttgg ccggccttgg ggctgcatga
    10261 gcgcgggaac atctcgtacc aggcactgaa gcgggccttc tctcgatcca cgacaacagc
    10321 gagttctcga tcgtacatcg ttgcgaagcg ccgatccggg tacttcgcca tcaaggtcgc
    10381 gacctctggg ctcaatgtca cgcggatgcg gtgcgactcc ggggtagctg cggaccggag
    10441 ttctgttacc catatggcga gctttcgacc gtcttccttc gtggcccgcc cgaccgcctc
    10501 atctatcagc ttggcgccga tgaggacatc aacctggaca tcctgtccgc catctatttt
    10561 cttgatgagg tctcggcgcc atgtgacaaa gtgatcgacc cagccggtca cggtgtatcg
    10621 ataccgtcct actgtggtca ctggaaacga ggcgcgccag cggtcgtttc caaggaactc
    10681 catcggaacg tccatccatt gctggtctgc ctcgtggcga tactggagca ggaccgagag
    10741 ggcgtcgtgc ccgtcggcga agacatctgc ttcgacgaca acgtcttcgc cgaccgtccg
    10801 cttgatgggg aatctcccac cgtcaatctc cggccgcacc cgctcgacga caacccgctg
    10861 tctgccgtat tcatcgatca tgcaatccgc gcttccttcc cggtcctgtt cacgcgtgtg
    10921 tcgatctaga tgtgcgacac ctcgtcgacc gtcgtcctcc ggcgcagccg ccgttccagg
    10981 gcctccttgg cgatcgtgcg tcctcccaag ccgagcgcga tagccaacgc caatacgatt
    11041 ccaccgaagg tgatggagaa cgcggcaacg acgatttcct tggcaattcc caactgcgtc
    11101 aagaccatcg cggctgtaaa gagaagaacg ccccatcgga tcaggttcgc gatgagctgg
    11161 gcttcctcaa tctgcgcatt gaccgcagcg atcagcgtcg cctcaccgct aaagttcgcg
    11221 agaagagctc cgatcagggc gacgagaatg gcggcgagca cttgaggcaa gaatccaagg
    11281 atcacattca tgagattggc ggtggccgga agatccagtg catcgatacc catgaacgta
    11341 aagagcagga acacgatcca aaaggagagc cggccgatca tgtgagagac cggctgcttc
    11401 atcccggctt ttcccagggc aggcgtcacc cccaaccgct cgcacaactg atcgaagcga
    11461 aacgtcttca acgtgcgcaa gagcagtacc ttcacgagcc atccgattgc caggcccagg
    11521 accagaaacg tcagcagcgc cgtgagcttc ggtaagaaga gcccgattcg tttcatcgtc
    11581 tctcgaaagc cctcaatcat tgcctcctgc cacatttcac tcattggaac ctccatcgcc
    11641 caaccaggta gggcttcaaa agctcaaacc actcacagac ttgttcgatc gtccgttcga
    11701 tctcgtcggg cccgccatct ttcgtctcaa gcaccagcga cgcggtacgc ccgagataca
    11761 gcggggtgag cgacttgagt aggtgctctc gatggataac cttgtcgcga tacgccaatg
    11821 caaagtcata gatgatctga acccagagcg ggatggggaa gcggaacccg tcctcatcca
    11881 ccaaccccag cgggagaata ccggctaacg tctcgggggc gagaatcatt tcccagattg
    11941 gaagcaaatc ccgcagccct tgacgaaacc ccttcaccat ccgctcgaca ttgatcacga
    12001 cggactcaga tccaagctcg tacgacggac cactcgttgg aaccggactc gaccccattt
    12061 gtttttccca tacgtcttga tagcgctcca tataatggta cgaggctccg accgcttgcg
    12121 ctagtaccgt cgaaacgtcc aatccgctcg ccttcgtgtc ctgaaccttg gttccgagga
    12181 atgcctgcca tacattgctc ccttcagcga ccgcgacggt ggtcagccaa ctgtcgattc
    12241 cgaagcgagc ggcatctcct tcccagacat ccctttgcaa atacagactc gccagttttc
    12301 cggaaaaccc atacccgccg ccagtctgac aggcgattcg ctttccataa agcgcgcggg
    12361 tcaaaggcgc aatgaggctg ttagtcagac tgccctcata tcgctggcgc tgaaacagcg
    12421 gcgcgacata gtcagcctct ttctccagca ccggccgcgc caatcgttcg atccattctg
    12481 gtgtgaccga tcggagattg gcgtcgatga cgatacaggc cttcgcctgc aactcctctg
    12541 cgatcgtaaa gaacgcacgg aacgcatgct ctcgtgccgg cacccccgat tccgacaatc
    12601 gaaggtgggc gaaggggttc gagaacatgc tgcccgtgac atgctggacc agggcgacgg
    12661 ggagcggacc tccgaccgcc cccttgacca tctctggcgt tccatcctgt gatccggcat
    12721 cggcattgac gagcaagatc gacgcatggg aaaacgaggc ctcgagtcca gcgacaatcg
    12781 ccttcatcac cggctccacc gtcgatgcat gatcgaacgt cggtacgccg acgaggagat
    12841 cgaccttgct gacgtcggga cattgttccc gcacctgctc ccacgtcgtc acttgcgtca
    12901 cggattcctt tctctgatgc gacggtccga agctcccggt gttccgtgcg cacgcccgat
    12961 ctgctctgtc accggttcgc gaacagcttc accagcgtca ggtaatcgat caaatggcgt
    13021 gtgaccagaa agtttctcct gacatgctcc cgcgccgcgc cgcccatgcg ttggataaca
    13081 ccggggttgt tcaaaagata tctgcctctg aacgccgctc cctcaacgga atgaaccgtg
    13141 taccctgtca ccccgtccac gatctgggcc gcaatgccgc cagctttgct tccaattacg
    13201 ggcttcccct tccacatggc ctcggacacc gcgacgccaa acccctcctt gatggatttc
    13261 tgaaggacga tcgtagcagc ccgttggagg gcgttgattt caaaatctgc ctcgggaggc
    13321 aattgcagca catggatatc cggatcacct ccggccgctt ccaggacctc tgctaacacc
    13381 atttcacctt ccggatcgtg cgtcacaccg gccccggcca ggatcagacg gcaatcgtgg
    13441 tgttttttga tcaatcgata aaccttgatc gcccccaccg gatccttgaa ccgctcgtag
    13501 cgcgccaact gcaacaggat gggtttatcc ctcggaatgc caagacggac aagaacctgg
    13561 ttgatttcca cacgcgacaa ctcccggttt ttctcactca atggatcgat cgacgggtag
    13621 atcaagaact tggtgatggg aagtctgtgg gcgaagccgg gaagcgaaaa gatggcagca
    13681 tcgtaccgga cgacaaaacg acgcagaaaa ctccagctgc gctgttgcgg cctggccagg
    13741 tcgaggtagc atcgccagat ccaggtgccg cccttccggt gctcgatcaa ggccaccggt
    13801 ggggggtcgt ggatcatgac gaggtccgca tccaggttca ggctctgggc attgcgctgg
    13861 gtgatcttga gatagatctg ttccaactgt tcggtcaggg tttcctcttg accctgcaac
    13921 gcgctggcca attgcttgct aatatggaaa aattcttgat cgcccgccaa gacttcccag
    13981 cgagcgtcca cgcccaacgc cttcatcatc ggcacaaggc gacggaggat ctcggccatg
    14041 ccgccaccgt atcgaaccgc gttgacgtgc aggaaactcc gccccgcgac caggtcactc
    14101 agccgataca gaaattcgac cgttcctttc ggtgcgtagt ctcgatattc gtccagttct
    14161 tgagccattc tattaccgcc ctccgactcc accagatgcg ctaaatgatg ctgccttcaa
    14221 ttccgtagga tccatcgggg ctcaatggct gctgcagttt gaaccagtag aatccgtatg
    14281 gagccagact gaacgcgtaa gggtgcttcc cgacagtcgg aaatcgcgaa ccgccgaata
    14341 gttcaatcgg gactgctccc tcgaatttag ccaggtccaa ctcaactgct tgcgccgatc
    14401 tggccaggtt atgcacgagg agcagcgtgt cgccccgata ctctcgcagg taggcgagga
    14461 cgttgtcggt ccgtggatga agaaaggcga gcgttccacg ccgaaacgca ggatgcttct
    14521 tgcgcacgga gatcatctgc ttcatccagt gcaggagcga gtgcggcgac tgtcgctgag
    14581 cctctacgtt ggtggcttga tacccgtaca ctgaatcaga cgtgaccggc aaatagagct
    14641 tcgacgggtc ggcctttgag aaccccccgt tccggtcagc actccactgc atcggcgtcc
    14701 gaacaccatt gcgatctccg agatgaacat tgtcccccat cccaatctca tcaccgtaat
    14761 acacgatcgg gcttccgggg agggtaaaga caaggctgtt aagcagttcg atcttgcgac
    14821 gatcattatc cagcaaggga gccaggcggc gggcaatgcc gatattgcgc cgcatctgcg
    14881 gatcacgcgc ataagcatag tacatgtagt cccgttcttc gccggaacac atttccaacg
    14941 tcagctcgtc gtggttacgc agaaacaggc accactggca attctccggg atcggaggcg
    15001 tgtgcgtgaa catgtcctca atgggatgcc aatcttcact gccgaccccc atgaaaagcc
    15061 tcggcatgag agggaaatga aacgccatgt ggaactcgtc gccatccccg aaatacggac
    15121 gcacatccgc gggccattga ttggcctccg ccagtagaat gcgtccctga taggtctggt
    15181 cgatccgttt gcggacttct ttgagatagg cgtgggtctc cgggaggttc tcgcagatcg
    15241 tgccctcccg ctcgaacaag taagggacgg cgtcgcaccg gaatccatct agccccaggt
    15301 cgagccagaa ggacatcacg tccagcatct ccttttgcag ctctggattg tcgtagttca
    15361 agtccggctg atggctgaaa aaccggtgcc agtaataggc tttcgcaacc ggatcccatg
    15421 tccagttgga cttttctgtg tcgataaaga taatccgcgc gttcccatac ttcttgtcgc
    15481 tgtcgctcca gacatagtag tcgcgcttcg gcgactgcgg agagctcctc gactcaagga
    15541 accaagggtg ctgatcggag gtgtggttca gtacgaggtc cgcaatcacg cgcatgcccc
    15601 tccggtgtgc ttcgttgagg aatgttcgga aatcttccat cgacccgtag tccggaaaga
    15661 ttccgcgata gtccgcgaca tcgtaaccgt catcacgcaa gggggagggg aagaagggca
    15721 gcaaccagag gcaatcgacc cccagccatt ccaggtaatc cagtttctgg atcaggccga
    15781 ggaaatcccc gatgccatcg tcattcccgt cataaaaggc tttgacgtga agttcgtaaa
    15841 acaccgcgtc tttgaaccag agtggatctg cccttggcac tgtatccctc ctctcattta
    15901 gcgcataggc tctcgagcca gaaactcatc gcacaccgtg agcagcgcgg aacgcaggcg
    15961 ctcgagactg cccaaatacg gattgatggc gcgtaatttc tctgccagct ccggcatgcc
    16021 gagactctgg cgaatccact cagagaagtc gctttcttcg cctttgaggc gatgccgagc
    16081 ttcgaagata tgaaaataga tcgcgctcac ctccacctcc ccgagcgcgt gccgaaactc
    16141 ccgcagcgtc cgtacttcca gtccagtcgg caccgccaga atacgggatt gattgaagta
    16201 gaacggctca ccgaaaatca ctcgcggcac gatcggcgtc ctggatagat ggtcgtcgat
    16261 aattgaaatg agttccgcgc gaagcgcatc aagtccctcg aactcgaaca ggtcgaccac
    16321 tgccagccgt tctcccaaca catggtcccg cacttccatc gccacccact gcgcaaaatc
    16381 gttcggatac acccgttcga catagctgtg cctcagaaaa taactgtgcg tgtgaaaata
    16441 gatcgaatcg agcggaaccg cttcaagcaa ttccaccaac cgcttttcgt cttccgcttc
    16501 ttcgccgagg atctctcgca gttcgctgca tccggtgaac cggaagggcc ggtctgtccc
    16561 gcgcatgatg ttctcttctg gcatctcaac ctgttatttg agagcgccga ctcaacgctg
    16621 gatcgggagt ataaggggca ctgatgagcc ccctgtggct gaagccttgc ggaaaatctc
    16681 gcagatgctc gccgctctgc gacagttcct tggcagagaa tccgacgtga ctcgcgtaac
    16741 tcaacatcgt ctcgaacatt aatcgggcct cctcgagtca actagccaga gcgtgcacgt
    16801 cgctcccttc actgctcaac gaacgtgccg acgcataggc ttgtcccgct cctgatgaac
    16861 cgttcctcca cgggattcga gtgaggctct gccgacattt tctcttccac ggtcgcatta
    16921 tacacattca gctcgagaac tccggaaaac gcgatagatt tcatggtcga cacggcatcc
    16981 gtctttcggt tgcctcaccc cgttcgaggc ggacaaggag aagcaaaaca gaggttgccc
    17041 tccctcgtca atggctcatc ggcgaaggtt ctgacaatcg tacgggcttc gcgttctgtg
    17101 acgtcggcgg ggtcgcccgt atccgtgacc cggcgactaa accaggcggc taccagcgcc
    17161 aacaggaaga ggacgaaccc gatcccgatc acgctggtat cttcgttgaa ccgctccgtc
    17221 agccacccga aaaaagcgat gccggccatc gaagtggccg tcgcgatcgc cgtatagatg
    17281 gccatgaccc gccctaccat gtgggccggc gagacttcct ggatcattcc ccaagcgatg
    17341 ggggtccatg ttccgaaccc catcccgatg ctggccatca gcaccgttgc catcatcagg
    17401 tcctgcgtcc aggtgagccc gcagagcgcc gcgcctccga tcacactcga caccgacacg
    17461 acttggaggc gcttgcgtac ctcccattgt gtcaccgaca cgagaccgag cgacaccaga
    17521 aacatcccaa tgcccagcca ggcccagaga tacccgacct ccaccgggcc caacgccagc
    17581 atctttttcc cgaagaccgg aaacagcgtc gtcaaggcgc tcgtcccgaa tgtgtacacc
    17641 gacgccatca gaatcagcac ctggatggtc ggctgggagc gtagcgcata ccggatcccc
    17701 tccgtcaggt cgccgcgcgt cgcggcgacg gggctccttg ggtgcgccat catggggcga
    17761 gggagctgga tcggcacgag acacagtgcc gccgcgacat acgtcaccgc attggcgcac
    17821 aggacctcct gtgacccgga gaaggcgatg cccaggccgc tgagcacggg ccccatgatc
    17881 attccgatgc tggttgtgct ctggagcagt gcattggccg ctgtgaaccg ggatcggccg
    17941 accagaaacg ggacagacgc ggacagtgcg ggcacgaaca tcgccgtggc aatgccatat
    18001 aggaagacca ggacatagag gctttcgacc gtgaacatgt ctgttgatac ccaacagggt
    18061 atcaacccga ttaagatcgc tcgcgcaacg tcgctgccga tcagaatcgc tttcttgggc
    18121 agccggtcga ccgagacacc aatgagcggg cctaagagga taggcggcag cgtctggagt
    18181 aagccgatga cgctggtctt cagcggcgat cccgtcacgg agtacacgaa ccagagaagg
    18241 gccaaccgac tcacgccgtc ggccacttgc gacagcacct gattccacca gatcaggctg
    18301 aaatcacgcg tcagcagcca ccgtgtcctc tcaacgcgcg aaccggtgcc acccgaacca
    18361 agttcgagga ttgtcggctg gctggcggtg cgttggtctt gaccgagccc cactgacgtt
    18421 gttctccccc ggcggcgatc tcctgggacg cgtcgccctg cggtcgtcac ctcagctccg
    18481 tacacgataa cgatgccgcg ttccggtggt caagaacaga gggacggaac agcacgctga
    18541 agagaaggag aaccgttgac cccgggatcc cggctaggtg accgccacct ccatgctcat
    18601 ggcttggcgg gcgaacaggg tcgccacccc cgccgtcagc aggagcacaa atccgaggcc
    18661 aacaagactc gccgccggtc cgatcgcatc cgccgcccac ccgaatcccg tcatgccaat
    18721 catggctgcc gccatcgaac cggtgctgaa cgtcgtgagg acccgcccca tgagatgcga
    18781 tggcgtcgcc tcctgcagca gcgcccacac caccggattg agaactgccc cgctggctcc
    18841 caccaggatc acgactgccg ccgcgatcag cggggattcc agcaggctga gactgcagac
    18901 cgccagtccc cccacagtcg tgccgcggat gagcaggtgc atccggctct gcacatcgtc
    18961 cttccgtctc caggccagcc acgaggaggc gatgagcatc ccgacgccca gagaagacca
    19021 gagccagccg agttgaaccg gttcggcatg caacagctcc ttggcataca cgggcagaac
    19081 aaagacaaag gcgctggctc ccagattgta gagggccgca ataatcacca acagaaacac
    19141 cgtcgagcgg tccccgaaca cgaaccggaa ccccaccgtg agatcgctca gcaccgctcc
    19201 cgaccgcgct gatatctcct gagtccgcca gcgtgtttca ttgaagcgga tgggcaggag
    19261 acacagcgcc gagatgaaga acgtcgcgct attcacgtac agcgcgtgtt gcgcgccaat
    19321 cagcgcgatc agcaccccgc tgatagccgg gcccaacagg atgccgatat tgttcgtgcc
    19381 ctggatgagc gcattggctg agacaagctc gcaccgcctg accagcaacg gaaccgccga
    19441 taccaacgcc ggaccgaaca ccgtcgagac cgtcgcagtc aaaaaaatca agatgtacag
    19501 cccttcaaga ctcagcaggc ccagtccata cagcgccggg atgaggaagg tcatgacgct
    19561 gcgagtcaga tccacccaga tcatcacggt tttcttcggg agacggtcga gatagacgcc
    19621 gatgaggggg ccaaagacca gtggcggaat cgtctctaag aggccaacca acgcgatcat
    19681 cagcgccgac ccggtgaggt catagacaaa ccagagcaag gccactttgc tcagagcctc
    19741 gcctacttga gacgtgacct gcccccacca gaggcaacca aagtcccagg tcttgactaa
    19801 gcgccatcct ctattgtcct ggtcggctga ttcggattgc atgtgtgttt ctcgggctcc
    19861 ggccactgtt ttggacggat catcgcaatg cggcgcccct cggagtattg cttggttaga
    19921 tgagcaagtt ccatcgtgtt tccatccagt caggctcgag ccttctcatg caggctctgg
    19981 acttcatgct gcagggaaga agtctgtggt agaggcggcg ccggtgggac cggcggcttc
    20041 ggcggaagtg ggatcggttg ggggggaagg ggcgacgttg ggcctggggg taatggatcg
    20101 gtcggcccgg gagccggatg cggtcttgga tctgcaacca tcattatcat ccctcccacg
    20161 atccctccag ccacactcac cactccggca aggagaatgc ctctcacaag agtcttcatc
    20221 gtgatgtccc tacggcacct gttgcgtatc gtggaacgcg tcgatctttg tccgtccacc
    20281 gcaaaagaga tttcggcaca aggcccgctt ccatcaggtg ctgtgggttc ctccgattcc
    20341 tctactaagc aaatcacaga cctggccact cggcgcacga cggctttaag taatatgaga
    20401 aaggatttgt atttcaggag gattgggtaa aacgctttgc agcgatagct ctaaagacga
    20461 gacgaaaagc aggacaattc gtcctcataa aagacaacct gtctaggccc cagctcacgc
    20521 atcgacctgg taagctgcgt gtcttggctt atggtgcctc tgattagtct ctgtattaca
    20581 gcaacatcca gccacatgtt cagcacatcg acttgccaat ctcgaatcta gtcacggttc
    20641 atcaacaaaa acgcttcaac tttggtcatg tgcggggcac cgggttggca ccccggaccc
    20701 ggagggagtg aaaattgaaa accatgatgc ctgcgagaat atagggcctg atctcatctt
    20761 attatcggtt cgttctcaat catttacaga ggagtcagag gtagacgaaa tgtagcaaca
    20821 cgttcgattt gaggtagagc ccctggtgcc gcaggggttg ctcacgagag ttgaaaatta
    20881 atcgtgcacc catatgcccc ggaattgact tgaaattggt cggcatataa atcaggggct
    20941 tcagtcataa atcctcctta gaatgcattt gaattcgccg agggatgata tttctcgctc
    21001 attaattggt ccatattatt caaacccgac tattggctca tgctgatgtg ttcagccaga
    21061 aatccgcaag agtggtcacg gataatatgc tgttgcggct agagggaccg aggcggctga
    21121 tccgaaggaa actattcctg acaccgtttt ctttttgcca agttgatgag acggagccgg
    21181 cactatattt ctgtacttga ttgctcgttc agccacgctt cgaacgcgtc atcgagcatg
    21241 cgtttatggt gcggttgata ccctgggatg atagagtaat ctcccgactc cagcatcttt
    21301 cgaatcatgt ccgcagtata cgacgcgcct tgctccttat gatctttgtg ccacacggca
    21361 agacgactct cagactcgcc tttctgagcc tcatcgaaaa cacaccaatc acagtctttc
    21421 gaaaatcgaa ggtaggccat ggcgttattc aagaactaca acaatgccgc gcgcggacga
    21481 ggcgatcaaa caaagactcc cgcccctttt ctgatcccct ttcgatccag ctcctcatgg
    21541 gctaacgatc accgtggcct gcattgtagc cgtatggaat tcgcagtggt acggataggt
    21601 cccgggtcgc gtgaaagtgt gctcgaagcg atccccgggc ttgagcagcg tcggcccgct
    21661 cgcgaacgcc tgccccccgc ctgaacaatc agccccgcgg cacgcaccac ttgtgacggt
    21721 gtgcggtgcg cgagaaccgt ccgcgtagaa ccagaccacc ttttcaccag ttctgatccg
    21781 gacctctcgg gggaagtaga at
//
LOCUS       DATIFR010000113         3833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1032469, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000113 DATIFR010000000
VERSION     DATIFR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032469"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..806
                     /locus_tag="VLD60_06300"
     CDS             45..806
                     /locus_tag="VLD60_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSB44603.1"
                     /translation="MGGHSHWATIKRHKGAQDAKRGKIFTRIIRELTIASRSGGDPDG
                     NPRLRLAIAKAKEANMPGDTMKKAIQRGTGELPGVTYEEFTLEGYGPGGTALLLEITS
                     DNRNRTVAEIRSLFTKNHGNMAEAGAVAWQFQKKGLLTIDKGKVDEDTLLSVALDAGA
                     EDVKVGEKLYEVVTNPHDFEAVKRALAEAKIDTTLAEITFIPQNTIRLQEKDAEQMLR
                     LMEIMDEHDDVQKVHANFDIPDDVMEKVAAAAAAG"
     gene            855..1463
                     /gene="ruvA"
                     /locus_tag="VLD60_06305"
     CDS             855..1463
                     /gene="ruvA"
                     /locus_tag="VLD60_06305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSB44604.1"
                     /translation="MIALLTGRLAFKAPTHLALDVQGVGYEVFIPLSTFYSLPDLNHT
                     VSLSIHTHVREDAIQLFGFVTQQEKDAFVLLTTVSGIGPKSALGILSALPLPDLVSAI
                     QSGDVEKLETVPGIGKKTAGRLVLELKDKSAKLHPALAHSSDLSSNGQDSTFDDALSA
                     LTNLGYRAQDAKEVLKQVRKSKVGPVSLQELIRETLKDLARG"
     gene            1467..1946
                     /locus_tag="VLD60_06310"
     CDS             1467..1946
                     /locus_tag="VLD60_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44605.1"
                     /translation="MTSTHRCSILKLSMMVIVLMLAPIPVIAEDTPAGPKSIGKTCGK
                     CPEGYATTGVTEAPGICKDGDPTLVQCVPLGANMLPVCGSCPEGYREVGSSSVPARCG
                     NQDGGRLSQCQLERMELNLPDPTKGGKICPPDCGSTPTPGQGALPPPPRYIPAPEKP"
     gene            1972..2976
                     /gene="ruvB"
                     /locus_tag="VLD60_06315"
     CDS             1972..2976
                     /gene="ruvB"
                     /locus_tag="VLD60_06315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSB44606.1"
                     /translation="MSDRIVNAQLTDDERTIEQALRPHTLDEYIGQERMKESLRICIE
                     AAKQRRETLDHTIFYGPPGLGKTTIAHIIAREMGGTLRATSGLVLAHAGDLASILTNL
                     QEQDVLFIDEIHRLPASVEEALYPAMEDFQLDLVVGQGPAARTVKLDLPRFTLVGATT
                     KAGALTSPLRDRFGLVHRLEFYSPAELKIIVMRSAGLLEIPIEPAGAEEIARRARGTP
                     RIVNRLVKRVRDYAQVKAEGRITKSVAQEALAWLGVDTAGFDEMDRKILLTIIEKFGG
                     GPVGVESLAAAVQEDKSTLEDVHEPYLIQAGFLDRTGRGRQATKLAFDHFSRPASQLF
                     "
     gene            3256..>3833
                     /gene="pheA"
                     /locus_tag="VLD60_06320"
     CDS             3256..>3833
                     /gene="pheA"
                     /locus_tag="VLD60_06320"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HSB44607.1"
                     /translation="MPGDLSDYRKEIDRIDDEILRLLNERSKSVIEIGKLKKQKDADA
                     NLHTPAREAAIIERLARQNTGPFPSEAIRPVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACTQKFGSSAQYVPVNSIKEVFSEVERGRAHFGVVPIENTTEGVVNHTLDMFIDSH
                     LLIYGEVLQEVSHHLLSKSGVPEEVKKICSHP"
BASE COUNT          874 a         1076 c         1119 g          764 t
ORIGIN      
        1 gttggacaag ccggtccgta ttgcaccggc aaatggagta agccatgggc ggacatagcc
       61 actgggccac aatcaaacgg cacaaaggtg cccaggacgc caagcgcggg aagatcttta
      121 caagaatcat ccgggagttg acgattgcat cccgttcagg gggcgatccg gacggcaacc
      181 cccgcttgag attggcgatc gccaaggcga aggaagccaa catgccaggc gataccatga
      241 agaaagccat ccagcgtggt acgggtgaac ttccaggagt tacctacgag gaatttacat
      301 tggaagggta tggaccggga ggtactgcac tattgctgga aattacgagc gacaatcgta
      361 acaggaccgt tgccgagatt cggagcctct ttaccaagaa tcatggcaat atggccgaag
      421 cgggggcagt ggcctggcag tttcagaaga agggccttct gacgatcgac aagggcaaag
      481 ttgatgaaga tacgctcttg tccgttgcgc tcgatgcggg cgcagaggat gtgaaggtcg
      541 gcgagaagtt gtatgaggtc gtcacgaacc ctcatgattt cgaagccgta aagagagccc
      601 ttgccgaagc gaaaatcgac acgaccttgg cggaaattac ttttatcccc cagaacacca
      661 tcaggctcca agaaaaagat gcagaacaga tgctcaggct catggagatc atggatgagc
      721 acgacgatgt ccaaaaggtg cacgcaaact tcgatatacc cgacgatgtc atggagaaag
      781 tcgccgcggc agcagcagcg ggctagcaga cgatctcatc atccatcgct tcaggagtct
      841 cgcacgaccg acgcatgatc gccttactga caggccgact ggccttcaag gcgccgacgc
      901 atctggccct cgatgttcag ggggtcggct acgaagtctt tatccccctt agcacgttct
      961 atagcctccc cgacctcaat cacacggtct cgctcagcat ccatacccac gtgcgtgaag
     1021 atgccatcca gctgttcggc ttcgtcacgc aacaggagaa agacgccttt gtgctgctga
     1081 cgacggtttc cggcatcggc cccaagtcgg cgctcggcat cctctcggcg ttgcctctac
     1141 ccgatctggt ctccgcgatt caatcgggcg atgtcgagaa gctggagacc gttcccggaa
     1201 tcgggaaaaa gacggcaggc cggcttgtcc tggagttgaa ggacaagtcg gcgaagttgc
     1261 atccggcgct ggctcattcc tccgatttgt cttccaacgg acaggattcc acgtttgacg
     1321 atgcgctctc ggcattgacg aacctcggct atcgggccca agatgccaaa gaagtgttga
     1381 agcaggtgcg caagtccaag gtaggacccg tgagtctgca ggaactgatc cgtgagacat
     1441 tgaaagactt ggcaaggggg tagccaatga cctcgaccca tcggtgttcc atactcaagc
     1501 tatcgatgat ggtaatagtg ctcatgctcg cgccgattcc ggttattgct gaggatacgc
     1561 cggcagggcc gaaaagcatt ggcaagactt gcgggaagtg ccccgagggc tatgccacaa
     1621 ccggcgtgac cgaagcgccg ggaatttgta aggatgggga tccgactttg gtccaatgcg
     1681 tcccgcttgg agcgaatatg ttgccggtgt gcggatcgtg ccctgaaggg tatcgtgaag
     1741 tggggagttc ctcggtgccg gctcgctgtg gtaaccagga cggggggcgg ctctcgcaat
     1801 gccaacttga aagaatggaa ctgaacctgc cggatcccac aaaggggggg aagatctgtc
     1861 cgcctgattg cgggagcact ccgacaccgg gtcagggcgc tctgccgccg cccccgagat
     1921 acattccggc cccggagaaa ccgtgaggcg gggccgagag accgagagag catgagcgat
     1981 cgaatcgtga atgcccagct gaccgatgac gagcgaacca tcgagcaggc cttgcgcccg
     2041 cacacgctcg atgaatacat cggccaggag cgtatgaagg agtcgctgag aatttgtatc
     2101 gaagcggcga agcagcgtcg cgaaaccctc gaccacacga tattctacgg tccgcccggt
     2161 ctcggcaaga cgacgatcgc tcatatcatc gccagggaaa tgggtggaac tctgcgtgcc
     2221 acctccggcc ttgtcctggc gcatgccggc gacctcgctt cgattctgac gaatctccaa
     2281 gaacaggacg tgctgttcat cgatgagatc catcgccttc cagcgtcggt cgaagaggcg
     2341 ctctatccgg cgatggagga tttccagctc gatctcgtgg tggggcaagg gcctgcggcc
     2401 aggacggtga agctggacct ccctcgtttt acgcttgtcg gcgccacaac gaaagccggc
     2461 gctctgacct ctccgcttcg agacaggttc gggctcgttc atcgcttgga gttttattcg
     2521 ccagctgaac tgaaaattat cgtcatgcgg tcggccgggc tgctcgagat tcccattgag
     2581 ccggccggcg cggaagaaat agcccgccgt gccagaggga cgccgcggat cgtgaatcgg
     2641 ctcgtcaagc gggtccggga ttatgctcaa gtcaaggccg aaggccggat caccaaatcc
     2701 gtcgcgcaag aggccttagc ttggctgggt gtggatacgg cagggtttga cgaaatggat
     2761 cgaaagatcc tgctcacgat tattgaaaag tttggaggag gtcccgtagg ggttgagtcc
     2821 ctcgcggcgg ccgttcaaga agataagagc acgctcgaag acgtccacga gccctatctc
     2881 attcaagccg gatttcttga tcgcaccggg cgcggccgac aggccacgaa gttagcgttc
     2941 gatcatttca gcaggccagc ctcgcagctg ttctaacagc gaatacggta ttcaaggccc
     3001 ggcaccggca acttgctccc ctggctcgcc cgactgtttc gataagccga aacactcgat
     3061 gccaaagcca ccccgttgat ctcgctcacc accccatgga attcattctg tacctcaatc
     3121 gcgcaagttc ctgaaactcc aggggtctcc ttgcatttcc gggaccggtt cctgtatcat
     3181 ttctctcttg ctcacacccg tcctgcctct ggacgctcgt ccaactcatg aggcacgggt
     3241 ggagggggtc cgttcatgcc cggagacctt tccgactacc ggaaggaaat agaccgaatc
     3301 gatgacgaga ttttgcgtct cctcaacgaa cggtctaaga gcgttattga gatcggcaag
     3361 ctgaagaagc agaaggatgc cgatgccaat ctgcacacgc cggcccgtga agccgcgatc
     3421 atcgaacgac tagccaggca gaataccggc ccctttccgt ccgaagcgat ccgtccggtc
     3481 taccgtgaaa tcatgtccgc ttcgttgtcc ctggaagggc ctcaaaaagt tgcctatctc
     3541 ggccccaggg ccacgtttac gcatatggcc tgtacgcaga aattcggttc gtcggcccaa
     3601 tatgtaccgg tcaacagcat caaagaagtg tttagcgaag tcgaacgggg ccgcgcgcat
     3661 tttggtgtcg tccccattga aaatacgacg gaaggtgtcg tcaatcacac cctggacatg
     3721 tttatcgatt cgcacctcct gatctacggg gaggttctcc aagaagtgtc gcatcatttg
     3781 ttgtcgaaat ccggggttcc cgaagaggtc aagaagatct gttcgcatcc cca
//
LOCUS       DATIFR010000114        59831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_266330, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000114 DATIFR010000000
VERSION     DATIFR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 59831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59831
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266330"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..680
                     /locus_tag="VLD60_06325"
     CDS             135..680
                     /locus_tag="VLD60_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HSB44608.1"
                     /translation="MIKALSVLCSWFILAIAAGPSQGQEAPPASPAAPETAAKATPAD
                     GHVIHVTAPHLIDGKVRGPFHHYCKIVSPEPFIECLLYETEDHNANLIGVEYIVAKTV
                     TRDEKVLPKKVWKKVWHDHSVEGVIGNIKILDMPPDKAKEFADTVAKTDGIIFALWPE
                     GAKVPTGKVSIGTMVGHAVHQ"
     gene            complement(1357..2046)
                     /locus_tag="VLD60_06330"
     CDS             complement(1357..2046)
                     /locus_tag="VLD60_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44609.1"
                     /translation="MTKKEGGSSARATFSLLILVLFLLGSFVLPSFNLSRSQAHAAGG
                     GQTKFQRISTQFIAALGDPGATSGSGAQSWGLWPLDPGPRGVELNSYKRLKDAGGVAP
                     ARWKFDGTDWWLEEHGLIMEQPTFPLPPGKYVVTGARDVTAVLTIHPADRNGDRRWEL
                     DKGATLYDVTHLACRSARYTPAAVGGLCSPANVQKTAFPVAPGGAMPPVEGCTKQDYA
                     VLIVIGVGVED"
     gene            complement(2533..2608)
                     /locus_tag="VLD60_06335"
     tRNA            complement(2533..2608)
                     /locus_tag="VLD60_06335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2573..2575),aa:Lys,seq:ttt)
     gene            complement(2654..3985)
                     /locus_tag="VLD60_06340"
     CDS             complement(2654..3985)
                     /locus_tag="VLD60_06340"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSB44610.1"
                     /translation="MGNLIVIGAQWGDEGKGKIVDILARDADAVVRYQGGSNAGHTVI
                     NDHGTFIFHLIPSGILYRGTLCVIGNGVVVDPASLIEEMDHLQGQGLKFGKNFVISQR
                     AHVILPYHKAIDKAAEQSKGSRRIGTTGRGIGPCYADKISRIGIRMGDLLNPKVFRKK
                     LEENLVEINWFLEQLYKVERFEVEKVYQQYMGYADRLRSHVVDATMLVNRMIDARKTI
                     LFEGAQGTHLDVDLGTYPYVTSSSSTAGGASTGTGVGPTKIDAVLGVAKVYSTRVGSG
                     PFPTELTDEVGAGLQDRGKEFGATTGRARRCGWFDAVVVRYATRVNGFTSLAMTKLDV
                     LDGCKELQVCTGYRYEGQLYRDMPADLQTLTDCEPVYKRFKGWSASTTGVTTYKGLPA
                     EAKRYLQYIEDIAECPIDMISTGSKRAATIVLKNPLKIPPRTGKRYGRVLL"
     gene            4216..4291
                     /locus_tag="VLD60_06345"
     tRNA            4216..4291
                     /locus_tag="VLD60_06345"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4249..4251,aa:Ala,seq:ggc)
     gene            4484..6325
                     /locus_tag="VLD60_06350"
     CDS             4484..6325
                     /locus_tag="VLD60_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSB44611.1"
                     /translation="MLQIESIHKQFSTKVLFDDASAHLRPHTRVGLVGPNGTGKTTLF
                     KMILGEESPGEGTIRKRPRLRIGYLPQELETITGKTALDAAHRDEYPEHEAKRILSGL
                     GFSEADFARPVENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFEEFLLN
                     SKLTLLVISHDTAFLDRIATHIWELRDKKIQEFRGNYTKFRELRAERDAQLQAAANRQ
                     AKEVARVQQFVDRFRYQANKARQVQSRIKQLEKVKLIELQRDTKRVKFKFPLPSTSGR
                     HVLELKGVSKRYGEKVVYSSLDFSVERGQRIALVGENGAGKSTLLKMLAGVLAPDSGT
                     RTVGHGVTLHYFAQHQAETLNPEHTILESLAEVSTTAETNFLRGLAGAFLFSGPDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDVLTDALTEFPGTIIFIS
                     HDPTFLTRISTRIVEIEEGNARNFFGDYEYYLWKKAQEFESIKESSEELIGKQPVKSS
                     GPTKAMAVQSQPKSTSGDRRDLNKTQARLEKQVARAESEIAEHESKIKERDQILADPG
                     LYKEFAKWNGLHQEQEIWKKELERLTARWESLSAELTDVKQKLEAFR"
     gene            complement(6322..6711)
                     /locus_tag="VLD60_06355"
     CDS             complement(6322..6711)
                     /locus_tag="VLD60_06355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB44612.1"
                     /translation="MKRPESKPTPAMVASNERRRLVRATLVGSALVSPKSGAKAITAV
                     LDNVNKVGAGLHAKEKLVMAEKVTVSLAFLDADRAEQQEKLDGTVAWVKPWEKGFLIG
                     VVWDEVVTKEKNKWLYYYLEETIKSSS"
     gene            complement(6763..8055)
                     /locus_tag="VLD60_06360"
     CDS             complement(6763..8055)
                     /locus_tag="VLD60_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSB44613.1"
                     /translation="MSAQHDQAPDLFSSPERARSVTAPLAERMRPCEFADFVGQEEIT
                     APDRPLRRAIETDRLSSVIFWGPPGSGKTTLAHLIARYTKAQFVPFSAVTSGVPELRT
                     IIKAAEQRQAINGQRTILFVDEIHRFNKAQQDAFLPHVERGTVILVGATTENPSFEVI
                     SPLLSRSLVVVLKPLSDEALGHILDRALSDAAIGLGKWNARLSPEARQRLIAFGNGDA
                     RALLTALEFVVTQAPVNADGVRAIDGIAVDAALARKSLRYDKSGEEHYNVISAYIKSL
                     RDSDPNGALYWLARMLEAGEDPKFIARRMVIFASEDIGNADPSGLLVANAVAQAVQFV
                     GLPEGQINLAHGTTYLATRPKDNASYVGLLEAIKDVKTHGNLGVPLHLRNAVTSMMRD
                     MDYGKGYRYVHDDPQASKEQEHLPEALKGRKYYRPKGA"
     gene            8169..8966
                     /locus_tag="VLD60_06365"
     CDS             8169..8966
                     /locus_tag="VLD60_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB44614.1"
                     /translation="MQARINGISLAYSDKGTGLPIVFLHAFPLNRTMWAQQEAVLSVK
                     CRVITIDLRGHGESDAPLWHYTLEQAADDVRALLDQLSIQQAVFVGLSMGGYILLAFY
                     RKYAERVKGLVLADTRAQADTAEGKEGRFQMAQTAYKKGPAAIADIMIPKLLSPVTVQ
                     TRTEIVQNVRAMIESNQISGIAGDLMAMAERQDSVPLLKQIACPTQIIVGGLDQATPP
                     SDARLMAEQIPGARLALIPNAAHLANLEQPESFNQILASFVSSLADR"
     gene            complement(8963..9610)
                     /gene="folE"
                     /locus_tag="VLD60_06370"
     CDS             complement(8963..9610)
                     /gene="folE"
                     /locus_tag="VLD60_06370"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSB44615.1"
                     /translation="MAKRGRAQGRRRQVEDVNGSGRPPDLEILQSLVTEMLLALGEKP
                     GRNGLIKTPERVAKAFAFMTQGYQRNVDHLLNGALFPIEYDEMVIVKDIDFFSMCEHH
                     LLPFFGKVHVGYLPNKKVVGLSKIPRIVDTFARRLQVQERLTVQIAETLQSKLNANGV
                     GVVVEARHLCMMMRGVEKQNTVAVSSSMLGAFRTQQQTRLEFLKLIRRGSVGDSD"
     gene            complement(9603..10031)
                     /locus_tag="VLD60_06375"
     CDS             complement(9603..10031)
                     /locus_tag="VLD60_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSB44616.1"
                     /translation="MAQVTVTRRYRFCAAHRLHTDYLTPEENWAAFGKCNNPNGHGHN
                     YVVLVTVKSGGSQEARDLERLDRVVEGAIVKRFDHHDLNQDPAFAEYTTTGENLVKLI
                     WDLLVTQLPAGQLEKVGVIETRDNYFEYAGAAQCIGGLHG"
     gene            complement(10148..10468)
                     /gene="erpA"
                     /locus_tag="VLD60_06380"
     CDS             complement(10148..10468)
                     /gene="erpA"
                     /locus_tag="VLD60_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSB44617.1"
                     /translation="MVTITAVAEQKIQELMKEEKDVVGLRIYVRGGGCHGYQYGMAFE
                     SKMADDDTVIEKGDVKVIMDSQSAPLLQGAEVDYVDSVQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            complement(10535..10882)
                     /locus_tag="VLD60_06385"
     CDS             complement(10535..10882)
                     /locus_tag="VLD60_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSB44618.1"
                     /translation="MPRVTFLHPQGKSGVVPRNTTLLDAAKALGFPLNHDCGGNASCT
                     TCRVEVQSGAEHLSEIDFEEQDLLDREALSEAWHRLGCQAKVLGDVVVRVPETKWAAP
                     TTEKSEAQGVDIR"
     gene            complement(10895..12199)
                     /gene="nuoF"
                     /locus_tag="VLD60_06390"
     CDS             complement(10895..12199)
                     /gene="nuoF"
                     /locus_tag="VLD60_06390"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSB44619.1"
                     /translation="MTTLVEPRLVQKLEGSPWDIDAYIKVGGYEAWKKCVKELSPDQV
                     VQELKTSGLRGRGGAGFPTGVKWDKVLNHRVKERYFVCNAGEHEPGTFKDRHLLKTIP
                     HQLIEGCLIASHTAQAKASFIYVNHEYHEERENLKKALAQAKERGFIGKNIMGSGIDI
                     DLEVFEGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDYGLYGKPTLVNNVETLCN
                     IPRIIHKGAAWFTQVGTEKCPGTMMFSLSGSINRPGVYEMPMGVTVRELIDTCGGGVP
                     GGRKIKAVFPGGPAFSMITADQLDLPMDFDSLKKAGTGLGSAGVIVVDDATCMVRQTL
                     KFSNFFKGESCGQCPPCRMGTINLATLMAKIEAGEGTQKDLDSLLQLCGFVKGTGYCT
                     LVTGASVLVQSSVKLFRHEFDEHIRLQRCPYPAVPVEAVAHH"
     gene            complement(12419..13006)
                     /locus_tag="VLD60_06395"
     CDS             complement(12419..13006)
                     /locus_tag="VLD60_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HSB44620.1"
                     /translation="MKFLEDPMQTMGAGFALALVLVVLFLGMSGVGAGEADWVGILFR
                     WVHFLAGITWIGLLYFFNLINAAFLKSLDGPTKNVVIPKLMPAALNWFRHGATVTVLA
                     GIALYFYMYSKGGTGAMALGIGGLLGIIMMANVHAIIWPNQKKIIAAVSAAAQGTPAP
                     AEMAQWGRTALLASRVNFMLSIPMLFFMGAGSHFK"
     gene            complement(13080..14018)
                     /gene="mdh"
                     /locus_tag="VLD60_06400"
     CDS             complement(13080..14018)
                     /gene="mdh"
                     /locus_tag="VLD60_06400"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSB44621.1"
                     /translation="MARPKITVVGAGNVGGTTAQRLAEKDTYDVVLVDIVEGVPQGKA
                     LDISQAGPVCGYSTRVVGTNGYAETAGSSVAVITSGIPRKPGMSRDELLVTNAKIVRS
                     VVTELVSRSPEIILILVTNPLDAMVHVARKVSGLPKTKIIGMAGVLDSARMRTFIADA
                     LKVAGPDVQAMVLGGHGDTMVPLPRYTTVKGKPVSELMSKEKLDAIVKRTRDGGAEIV
                     GLLKTGSAFYAPSASAVDMVEAIHKDQKRVLPCAVFCEGEYGLHNVVVGVPVKIGRGG
                     AEQVVEYELTTEERTALEVSANAVRELCTAVDRLMA"
     gene            complement(14110..14517)
                     /locus_tag="VLD60_06405"
     CDS             complement(14110..14517)
                     /locus_tag="VLD60_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44622.1"
                     /translation="MANSAERPTVPPWLYKLFTGHQYPYVRRLAKFAQPVKPGEDRQE
                     PTKEMVEAKFWEVYPRCSAKILQEVKAGMIVVFHDLGEFPPGGFQELVDNPEAFLAKT
                     YGKKKIKVNFYDGENFVCTINFKVAGWTEHEHA"
     gene            complement(14561..17590)
                     /locus_tag="VLD60_06410"
     CDS             complement(14561..17590)
                     /locus_tag="VLD60_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB44623.1"
                     /translation="MADLPAQKALIDRLTGELGAAISNAVVARLAESAGQADRVAALL
                     ALLDELEEVSSKAARAAIEALPELDRRAGLSQVIPWLDLGVALAESSGATALKYFKDS
                     SLILGLIEQADARASILAIGLEIADEDANVTLEYLRTAPQILAVVPSEQLKPWLEISV
                     ELTKVDVVVGLEYIRQIPALAPVLPLGEVRNWLSFGMKLIAPNTIGKPDYMATMEYLR
                     TSPAILGDIEHIPVRSKVVALGALLAERSPEAGVAWLAESPRLLRALPTMEWQVKVLQ
                     YGSLLAEKDAQAALSYMRQCPEIVGLIGDVSQALSRFENWFKAGMEVLAYSPEGARAY
                     FAMESQKALSSVEQALSGVPLRQVARKIKLFVQGLCGTDVSITALPESGTALATRAMV
                     SANGRTIALPALLRRYPTAEENERLFLIMAAHEAGHLEFGTYRLTMEPLTDLVETVCR
                     RYGRSQQTRPDSLAALFRLYPHPRLIHDLWAVLEDARIEFLLQAEYPGIRRDLARLAA
                     EAITPRDPAHGLTAKELIVDCLLRLSTGESEATAVPHAVIEEVLILWNLCQPVFATTA
                     TAEKTVRLVDTIYVRMEELLAPRAEMITAAQAKEDAQELGVGPTASENQSEVYRPVTN
                     WVYRGEMNPEFIKRDQEQAEESHADLDRMASQGGGSKERSGAGQGSRRDEQESISGDM
                     LGGGRSLPSVVEELLALDVEQQPVSESTGGGERAVRYPEWDCSIQDYRMNWCRVIERS
                     ADAGSDDSVSAALSAHRSEIKSLRRFFEGLRPPAFRRVAGQADGEDVDIDAVVRRATE
                     QRAGFEGSDRVYVRHEKKERDVAVAFLVDVSGSTSRQVENGRRVIDVEKESLVLLCEA
                     LDAIGDQFGLYAYSGQGRASVDFLTVKDFDERLGPAAAHRLGGLGPRQQNRDGAAIRH
                     AISKLKSREARIKILILLSDGRPLDGDYKDEYSLEDTKAALREARRQGIDPFCVTIDH
                     EADSYLRRMYGDVQYAVIDRVEALPAKLPRIYRQLTT"
     gene            17694..18119
                     /locus_tag="VLD60_06415"
     CDS             17694..18119
                     /locus_tag="VLD60_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HSB44624.1"
                     /translation="MAEPKQESLDKMWKYVKGFAEKSGTTMHPNPVVTEAVVKGLAMH
                     VDELGKPLCPCNFYKDKGVEAKLRRWICACDEMQIYKYCHCLLFVREDGMPITEYLPE
                     GHEGREVYGVITDPSPDKGRALKHKAISTSLPAPETQKR"
     gene            complement(18137..18565)
                     /locus_tag="VLD60_06420"
     CDS             complement(18137..18565)
                     /locus_tag="VLD60_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44625.1"
                     /translation="MMKCKSVLVMTLTMSLLACAGPEPMLRSNAKVQLEGREASKLDV
                     VACQEKAEAAGLQHGTNRTSNAASGAVLGLVGGAAAGASAGLIGGASGAAIGAAVGGG
                     LGLIIGLAGGAYKPLEPDPPYADAVVRCLIEKGYEVSGWQ"
     gene            complement(18764..19189)
                     /locus_tag="VLD60_06425"
     CDS             complement(18764..19189)
                     /locus_tag="VLD60_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HSB44626.1"
                     /translation="MTRSLRYLVCGVVLLNACSSAHPILYPNTHFKSVGKEVAEQDIE
                     ACKQLAETSGAEEGSGKEGQVATSTAVGAGVGAASGAVGGAIVGAAGQGSAIGAASGA
                     VWGLLMGLFRAGSSQPNQAYTNFVNRCLQEKGYEVTGWQ"
     gene            complement(19186..19497)
                     /locus_tag="VLD60_06430"
     CDS             complement(19186..19497)
                     /locus_tag="VLD60_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSB44627.1"
                     /translation="MTPRIVQDSKDGAVLTVHVQPKASRTACVGIHGDALKIRIAAPP
                     VDGAANEELIRFLAQTLSLPLSAVHIDSGASGRHKRVRLDGVNAAHVTARLLEKGPAA
                     T"
     gene            complement(19518..20336)
                     /locus_tag="VLD60_06435"
     CDS             complement(19518..20336)
                     /locus_tag="VLD60_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HSB44628.1"
                     /translation="MTDKGREIDIGHYRIQQEPFYAEVRGEVDLFTTAAKNRMPVMLK
                     GPTGCGKTRFVQYMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWVDGPLTLGV
                     KHGAIVYLDEVVEARKDTTVIIHPLSDDRRLLPIEKKGQVIEAADDFLLVISYNPGYQ
                     SVLKDLKQSTKQRFMAIEFDYPSPDIETAIVQREAGVAADVAASLVKLGGKVRNLKNH
                     GLEEGVSTRLLIYAGTLIREGVAIERACDVAIARPITDDPDMQRSIMELVKAIF"
     gene            complement(20451..23951)
                     /locus_tag="VLD60_06440"
     CDS             complement(20451..23951)
                     /locus_tag="VLD60_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB44629.1"
                     /translation="MSRQDFQRPLDSQRYRWLPQLIILMNLVALVAGAFLLRNVEHRL
                     IVAAGEELTIAAAEVSDKLDRMLFQRFGDAQMMARAFALRSSDARYVSSYLEWMKENY
                     APIYLWLGVTDGQGVMVASTEPSKRGRDYSRADWFQRARETRKIQADDIAVHDGDGGV
                     ETVAFTAPILDSEGTFLGVVTTRVETSVLEEVTTKTVRSLEGRQEFTGPVAIQMVTKQ
                     GTVFVDSDVMHKGLLNTQQSGHRSVTASHFGEPGFVEEEYQGESVVTGYAHTKGYGEF
                     PGHGWYVLVRMDRDRILDPIKSIMWKFGLAGAVLWVPMMLLLFWSTAKLRAEYQQAQQ
                     ESSWARAAEAALLQSQERTRAIVETALDGLVTIDAVGVVTDWNSQAAAIFGWSREEAL
                     GQRLAELIIPPREREAHERGLRHYLASGETHILNRRIEVLALHRSGNEFPVELAISPV
                     RIGDTIFFSAFIRDITVRREAETKLKESELRYRSVVNALDEGVVVIDAEGMLKTANAS
                     AERILDLSLEELTGRSVRDGRWQTIREDGSPFPLEEYPALITLRTGQPCASAVMGIYR
                     PDGKLRWIDINSRPLAAEGRMTPASVVVSFSDITERKRADERLTAQYAVTRVLAESRT
                     LEEAVPRIMQAVGQNLEWDCGIFWRVDKATGVLRCLDQWHSAVLKADVFLTDTWERGF
                     KVGESLPGRIWANGKAAWISDVVEDESFSRRKQAREIGFHGAFGFPIRVGGEVEGVIE
                     LFSRQVRVPDEQLLKMIDDVVLKLGQFADRARAEEALQQTETQLRQSQKMEAVGRLAG
                     GVAHDFNNLLTVIRGYTELLLGRLSQDDPMRKDMEEVKKAADRASGLTRQLLAFSRRQ
                     FIAPKVIDLNALIANMDGMLRRLLGEDIVELCTELTPHAGAIKADPGQIEQVIMNLAV
                     NARDAMPKGGRLTIETHNVTVGNPTRQNPSVVKPGAYVLLAVRDTGHGMDEETQSHLF
                     EPFFTTKEKGKGTGLGLSTVYGIVKQNGGSIVVESKPGQGATFKIYFPLIEQGAQNRG
                     GQQDITERPYGRETILLVEDEPAVRGLVQETLRLHGYTVLEARHGIEALMTGAKHQGP
                     IHLLLTDVVMPQMSGPEVAEKIQVVRPGIKVLYMSGYPDHPAFERGGILRPSSFLPKP
                     FTPNVLAKKVRDVLDGVQVA"
     gene            complement(23948..25453)
                     /locus_tag="VLD60_06445"
     CDS             complement(23948..25453)
                     /locus_tag="VLD60_06445"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HSB44630.1"
                     /translation="MNSLTTKTASPAAATRPDLPQFVPSSTCLRCDICCRFPDPDSPL
                     RPYFTKEEVDQAIRGGVDERVFSDRGGCQILVVPDPEGEGFHCPAFASQTGTCRIYER
                     RPLDCQLYPLAIMWNAVQDEVLLGWDAKCPFMPEQLPESIRLHAERVMELLRQPEIVR
                     MIVNHPGLVGRFQEDVVVLGPLTHLAQALMERWGAHPVHRLLLEDLPRLTNALNRSGL
                     AGVRPLAAHSASYHYVWNGLLAYWWTELHGAFCLFIQSPDGWFMPLPPLKEGSILNAI
                     EEGFQLMRRRNGMTAVSRIENIPAVLAAELEAVGYRMTPKEPDYLYRAADLAALAGDR
                     FKSQRALCNRFERIGDVTSEPYHPRDRAECRALFHEWGQQKRTGGLDTFATHLLEDVL
                     SAHEVVWAHGSELSLHGMVLRLKGRIRAYTFGYWLSKDTWCVLLEVADREISGLAQYL
                     FRDTCRRALSEGAEFINTMDDAGLSGLRLSKQAYHPMMQIQGFICSEAMRA"
     gene            25624..26637
                     /locus_tag="VLD60_06450"
     CDS             25624..26637
                     /locus_tag="VLD60_06450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSB44631.1"
                     /translation="MANSPDLVQYDPQVLLNSQPVIITVIDPLTHKVVFQNQTSQNKF
                     GDISNLTCHEKIAGCATPCAFCRMPECFESGNPSTSEVSIPNEEHLLVQWAQVKTASG
                     QTHIVETITDITAIKRQQAEAEGLVQRLSSTNRDLIQANQLLRDQSIRDSLTGLFNHS
                     HFQETLTQLCALSLRSMRPLSLLFIDLDNFKQINDAYGHTMGDQVLRELGWLLDSQQG
                     PGRGIARSSDFSARYGGEEFALILPDTSMEGALSAAERLRLRVTTLTMLPELTALNSK
                     PCSLTCSIGVASFPIHASTSSDLIIAADTAVYTAKKSGKNCVRMATPANPSLPLFDPL
                     VRS"
     gene            26850..28205
                     /locus_tag="VLD60_06455"
     CDS             26850..28205
                     /locus_tag="VLD60_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44632.1"
                     /translation="MTPLAITPDQLTFFQALGPQLLLFYGAVNRLYAESVRRTQPAWI
                     ADYLDQGKPEGLITYSRMKRFREHLPAVIRPDIIPTQDGMVITELDSVPGGIGLTACL
                     SHAYSDISSQHSALSTHHMGLIGGRDGMIDGFAAMLQAQRGQLNGVIAILVSEEAKDY
                     RPEMTWMAAQLSARGLETYCVEPRQIRFTEEELLITTPERQIPVGLVYRFYELFDLPN
                     IPKAELIQYAAKKGWVSVTPPYKPALEEKSAFGLLHHPMLQPYWRQELGEDLFLHLTT
                     IMPRTWILDPAPVPPSATIPGLSIGGRATMSWSDLAGATQKERHYVIKPSGFSEFAWG
                     SRGVSVGHDLPQGEWAAAIDSALKAFPRTPYVLQEFHKGRQYRLEYFDSATEEIKEMA
                     GRARLSPYYFVSGEKAELAGILATICPVDKKVIHGMKDAIMVPCAVAAEAHQTKDLSA
                     E"
     gene            28271..28525
                     /locus_tag="VLD60_06460"
     CDS             28271..28525
                     /locus_tag="VLD60_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSB44633.1"
                     /translation="MSLTLYHVDWCPECDLVRQKLAELNLPYTDVIVPDFRPLRKQVF
                     EVSGQYYVPVLQDGDKVLSETHEILAHLERKAESDRLDQG"
     gene            28541..29734
                     /locus_tag="VLD60_06465"
     CDS             28541..29734
                     /locus_tag="VLD60_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HSB44634.1"
                     /translation="MEEWRRILAESIVKPKDLADRFGLDENEVEAIVGPYPMRITPTV
                     LGTIREKGDAIWKQVVPDVAELADIDAEDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAITYLRQHSEVRDVILSGGDPLLLPDHLLER
                     VLKALRTIPHLELIRIGSRVPGTLPERITPRLCELVKKYHPIYMNLHFNHPDELTPAV
                     KAACGMLADAGVPLGAQTVLLKGVNDDPETMKRLVHQLLLARVKPYYLYQADLTKGTN
                     HFRTSVETGLNIIKALQGNTSGMAVPHFVIDAPGGGGKIPLLPDHYLVHLDDEGAVLR
                     NYENKTFHYPQPKAGNSRELPMVGARASYDDSNEAFASCGDSFPDRDGYSSWGNSCGG
                     ESDDL"
     gene            29731..30885
                     /locus_tag="VLD60_06470"
     CDS             29731..30885
                     /locus_tag="VLD60_06470"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HSB44635.1"
                     /translation="MTKNAAQGILPYQQIRRLVTGGAITASVGIEDRQIQPASLDLRL
                     GKKAYRLISSFLPELSAISSRLDVMDFYQSDLVMYEMDLTGGAILEKGHVYLVPLLES
                     LKLPGTIRARANPKSTTGRLDVFTRIVTDLTAGFDEIRAGYRGNLFLEVVPRSFAIRV
                     KTGQSLNQIRFIRGEAAASDATLQSLHRHEPLLYHNTPNRKPLRAKDFRTDRGLFLRI
                     DLKGEEGEGSTVIGYRAKKNSHVIDLSKIGHYAAADFWEPLYRHRQDSLLLEPEEFYI
                     LASKERIRVPPDYAAEMVAYEAACGELRTHYAGFFDPGFGYGADGEIKGTQVVLEVRP
                     HDVPFLIHDGQTFFKVVYDRMLDVPDQLYGVGLGSSYQQQALTLSKHFKV"
     gene            30889..31302
                     /locus_tag="VLD60_06475"
     CDS             30889..31302
                     /locus_tag="VLD60_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44636.1"
                     /translation="MAPTDLPPPHDPAQRPPAQPTGSEGQDSGSDHQMHVVGLMVGTA
                     LMFIGFLNVFLSISGGFEINVVPFLIYFGGLAVWANAAIETPALRYGVMTAAIALALG
                     FFHYGEVLFWHKQVVFWSTVVIVMYFMFNEPKKPD"
     gene            31313..32020
                     /locus_tag="VLD60_06480"
     CDS             31313..32020
                     /locus_tag="VLD60_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSB44637.1"
                     /translation="MTISVIIPTFDEAITISETLSHTASLGFDELIVVDGGSADQTRT
                     IVETFISGIQHSALSTVRVLSAPCCRAAQLNAGAQASRGDVLLFLHADTHLPETAKQR
                     IAAALADTHIVGGRFDVRFDRPSVLGRIISTLMNYRSRWSGIFTGDQAIFVRRDIFER
                     LGGFADIPLMEDVDFSARLKRAGPTEALRETVVTSFRRWEQQGPLRTILLMWTLRFLY
                     WMGVSPSRLVAWYRAVR"
     gene            32038..32838
                     /locus_tag="VLD60_06485"
     CDS             32038..32838
                     /locus_tag="VLD60_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSB44638.1"
                     /translation="MSSLRNRQLHASRPESSSSPHASRLTHHAAALVIFAKAPIPGQV
                     KTRLCPPLTADEAATLHGSFVLDTLERTKAAAGKLKLPFDRYLACSPSSTAVFFKIME
                     ERQGVKLIDQVGEDLGARMKHAFETLFARGYRHVLIVGTDVPSLPLAHYQQALAFLDA
                     HDLVLGPALDGGYYLIGLNKPTAALFENVSWSTDRVLSMTRERADGLGVRTALLPAWR
                     DIDTIDDLNALIEAGALDAKKPKNEQSFSSRTAGALQLIAKRLRSRAT"
     gene            32848..33582
                     /locus_tag="VLD60_06490"
     CDS             32848..33582
                     /locus_tag="VLD60_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB44639.1"
                     /translation="MSSHVAVITGGAKGIGRGIALDLAAQQWNVAFCYRTSEAEAKKT
                     AAAITERGGQPLAIRCDVSDPVAAKNFIARVEKEWGQIDALINGAGPYHRVNLFEETV
                     TGWNEMFEGNLHPIFYLAQAVAPGMKARKSGRIVNFSMANADQMISQPDVTAHYIAKA
                     GVLILTRTLAKLLAPHGITVNAVSPGFIDSGSAPPEELAGMTKRIPAGYIGTVEDTVA
                     AVRYLLSDQARYVNGANIQISGAWGI"
     gene            33715..34119
                     /locus_tag="VLD60_06495"
     CDS             33715..34119
                     /locus_tag="VLD60_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44640.1"
                     /translation="MKTEQERQAHHRFVQALQHEHLTCAKPGCGGPMDVVDLTPHNAR
                     IKTYEAECERCRMKEHITGTEQNQPPWDDASITMMAEVHLLHDQPTCPYDDTPITFIS
                     MPNPRRKARYRLACFYCGRHTEMNWPPPEAKR"
     gene            34305..35837
                     /locus_tag="VLD60_06500"
     CDS             34305..35837
                     /locus_tag="VLD60_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44641.1"
                     /translation="MNGIVLEQIAVDPAGAVETLKLFPTFPNRVHHFAHLASVYAKAG
                     RVDEVERMYAEILVEDRSSREGKLAAANALGQLAIAHANTGNMEEAFRTLFRIKERSK
                     EESLAIVDIATAGIADAQARHGDIPGAVQTALSIVRENPYPLMKIVGSRALKGSAGGE
                     VEEIVSSLDEGAQQYAQWGIVQARIQQGRFTDAQVAASAIKPGHAKAGALLELATRHI
                     QLRSKPLAVVLLQEAATSARSTINEWTRADILWHIAAGMAEAGDAPAAIDTAKSIEKD
                     GHRISALHDIVRTQAKQGDIAGAFNTALQLKPAFNIEFPGSSTYEVAVSDVLVQLVKS
                     GKAQEARETVERLQDMQSEHPLLYSAITTAQADTGNIKAAKTTLALVETERQRKARKQ
                     ELLDLAEKFREGQNPDDDSRWRKVQSLDVAIRQAQEAIAKAYARKGDLASAIATANEL
                     NLSSERLRLIKEISALQTQTAATESALRWARGLPSPSDKAYALLGIATGLSEVKNKKT
                     PK"
     gene            complement(36327..36650)
                     /locus_tag="VLD60_06505"
     CDS             complement(36327..36650)
                     /locus_tag="VLD60_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSB44642.1"
                     /translation="MAETMEEEIQNEVKAHKILIYGKGTKTMPMCGFTRETMQFFDKY
                     GYPYEVIDVLSQPAKREALTKMTNWPTLPKVFIDGTFYGDTDVLDPMAAKGEMEPLLK
                     KAFGK"
     gene            complement(36698..36937)
                     /locus_tag="VLD60_06510"
     CDS             complement(36698..36937)
                     /locus_tag="VLD60_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HSB44643.1"
                     /translation="MISQETLTDYIRRAMPDAMVTMTDRTGTMDHLKVIVVSDTFHGK
                     NLLDRHRLIYQALDAPMKDGRIHALELTAKTKDEL"
     gene            37588..38022
                     /locus_tag="VLD60_06515"
     CDS             37588..38022
                     /locus_tag="VLD60_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44644.1"
                     /translation="MNEFTSGFFMGDKETNGRVLVVDDEADIRKVVRMTLQKAGYDVL
                     EAENGEKAIETINAGENRLLLDVVICDIRMPKINGVEAIAYFRSNYPRVPVIVLTGFP
                     DTEMATSMLRQGVVDYLVKPVEGEKLREAVSRAMEQRELAHL"
     gene            38044..39159
                     /locus_tag="VLD60_06520"
     CDS             38044..39159
                     /locus_tag="VLD60_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44645.1"
                     /translation="MGRTSQLASPPPEVNAVQHLTDRVVVSKIAPTKVAILGAGRGGS
                     ALLDLLHQIPSIEIVGITDRDLRAPGIQRAKDLGIPVVAQAEELVSHDGINLIMDVTG
                     DPSMERFVHANKRPEAEVLSGPASRVLWELAQHEAKLQAELFHAEKLAGIGSFATGIA
                     HDINNPLQLIMGLAENLADEKDLDTIHEQAREIIGAVRRTSAICRDLTQYARRKSDSG
                     ETMVYLNGRLDEALKIARYAVSFHDITVLKEYQQGAVAKGNADELLHAFVNLITNAVH
                     AMDHGGGMLTLATAISDGGVTVQITDTGCGIPKEMLGQIYEPFFTTKEPGKGTGLGLY
                     NVKTIVTKMHGKIAVKSRVDHGTTFTITFPPVPEAPS"
     gene            39156..41669
                     /locus_tag="VLD60_06525"
     CDS             39156..41669
                     /locus_tag="VLD60_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44646.1"
                     /translation="MTQPPGKSSSSTRGWRLQTKLIVSMLLVGVVPLLVGLGMAFWQG
                     SKEIQEVSGESFKALATEAARKIDILLAEEIARSSRITTDPFIVRELERRRDALQDPA
                     TSASYDDLGRKWDSQDPATLKALTENPIANLLREYYSGARSEPDQLIPQVVRGATKMF
                     FVTDIRGDLAGALTTKPPFSHGKTAWWQGAFNKGIGQLYIEDVHYDDRAGTYAFTISL
                     PIMDSLRYEAVGVLHRVIDAKEFFSPSTQPIRFGKTGHVMLIDSRGVVMSCPILPTGV
                     SLSDSSLIPLVTPMHPGWTSAPSDGHGGRSSSIIGFAPLPETSRATNGAYERGSWHTF
                     VWQSSDELFSPIRHLFTWMTVFGFIAVGLLATLGYLAAIRIVTPVRQLQQAAQSIGRG
                     ELRQSINIKTGDELEALAEEFNRMNKQLEAAFAGLTDQVTLKTQEVQYLQRSTDQILD
                     AVPTPILIVDDQETVQYVNRTGRQAFGLAEDSASTGRLFDILPVESSQQERLRQELWK
                     HQMAGTASLPKIEESAAVDHVRDPLAPGTGATQRERQGELRLDAHVYQYQWFRVIGRP
                     GESDRIGLVLRDSTDDSRMQEKLIQAEKSGSLGVLTAGIGHELNNPLFGILGLGEALQ
                     EECDPARIRSYAQDIVQQGKRMASIIRDFTGVAARETSDQRQSVHLERELDQALATIT
                     ASLGTPELTVAKQYAGDTTVSALPDQLRQAFTNLLMNAVQAMKGRGLLRLSTVVAGTS
                     VVTTIADTGPGIPKEHLSKIFDPFFTTKGQGEGSGLGLTVARRIIRKFGGDIQLESVE
                     GTGTSCMVTLPILSSTPSSKEVPWTASESRVLPSQPSRS"
     gene            41618..42214
                     /locus_tag="VLD60_06530"
     CDS             41618..42214
                     /locus_tag="VLD60_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44647.1"
                     /translation="MDRFGISRAAVATIALLTVGLWWGTVGSSAKEELKTAGIPPEKV
                     AGFVHAVLQAHRTIYTTQIVNRLQEKGIVAAAEHWEQENALPLPAQFLQHSGRLVAES
                     GQGIRYRLIGLSPIYQRNAPSTDFERKALESLNRSPDAPVTGIVTSGRKQYFQAIYPD
                     RAVSSACVNCHNSHHLSPKRDFKLNDVMGGIAITIPIE"
     gene            complement(42308..42751)
                     /locus_tag="VLD60_06535"
     CDS             complement(42308..42751)
                     /locus_tag="VLD60_06535"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSB44648.1"
                     /translation="MKKNGFGGDDNITLLAKGVQLKGEIRVEGTVRIDGRLDGDIHTK
                     GQVIVGEDGLVQGTIAAGTVVSSGRIKATVTAVERVQLLKTAILIGEVHTPVLMMEEG
                     AKIQGVTDMGVTAWPDELPKLPGAVRDLSSHRAKQGVVLDKESNG"
     gene            complement(42873..45197)
                     /locus_tag="VLD60_06540"
     CDS             complement(42873..45197)
                     /locus_tag="VLD60_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HSB44649.1"
                     /translation="MLNKMLTRLSHILRSPWFFRGGLIVAGMVVLLIAGLALYGAYLA
                     TFLELPKSEDHPPLRLYTAPFQLKSDLSVSAARLQERLQRLGYRAVSGSVAAPGDYQL
                     TPQSLTIYLHAQPENLTKASIVSLELEQGHVTQVLSEPDRSPIFPVFLEPELISGLRG
                     TSRQVREWIPLSQIPPRLVDTVLAVEDRRFYSHIGIDPFAIGRAVWRNLTKGGVVQGG
                     STITQQLAKNLFYSPQRTFVRKVKEALAAVVLETKYRKQEILESYLNEIYLGQAGFVS
                     IYGVGEAAHRYFGKSLDELTAGEIAMIAGLIKGPNTYAPTKHLELAQQRRDVVLRLLR
                     DQGHLNEEEWKIAVNQPVRVAPPDDVLADAPYFVDTVLRQVEDAIGTPLPEGLRIDST
                     LDPLVQQVAAEGLENGLARLERAYPALKSSEQSLQGAVVVLEPSTGAILGLVGGRDYR
                     VSQFNRAVQARRQAGSLFKPFVYLAAFEASRMGKQPYLTPASLIADEPVTFESENGTW
                     SPQNYDRQFRGNVTIRSALEQSLNVPAVRVAKAVGTQPILEMIRQLGVTTALTDDLSM
                     ALGSPAVSLLEMVAAYGTVANGGIWVRPTSLRATSDREGTALWSAAPDRRQAVSPQAA
                     YLVTSLLEGVIQRGTATKAKVLGLNGAIAGKTGTTDGYRDAWFVGYTSDVVIGVWVGF
                     DDERAVRLTGAQAALPIWMEIARRMLPDHAPPFPRPSGVLVRAIDPKTGQLATSQCPE
                     QVDEVFIEGTEPDVYCEIHGGGIWERLRQTFGFS"
     gene            complement(45262..46677)
                     /locus_tag="VLD60_06545"
     CDS             complement(45262..46677)
                     /locus_tag="VLD60_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB44650.1"
                     /translation="MILIFVILYLFVSVSIGLYAATRVRNAKDFAVAGRSLPLPVVMA
                     TVFATWFGAEAVMGISATFAKEGLRGVVADPFGSSLCLIIAGLFFAPRFYRLNLLTVG
                     DYYRLRYDRRVEVLCTLCIVASYLGWVAAQFKVLGLVLNVVTDGAVSQKLGMVIGALI
                     VLTYTTFGGMFSVAILDFVQISVIIGGLLYIVSVMSDLTGGVGTVIAHAAQAGKLEFF
                     PPATLNAWIPFIGAWVTMMLGSIPQQDVFQRITSAKNERTAVRASLMGAALYFSFCFV
                     PMFIAYSALLVDPEKFGALLQRDSQLVLPTLIVEHTSAFAQVMFFGAVLSAVMSCSSA
                     TLLAPSVILSENVIKGIMLHMDEWALLRTMRIVLICFAAVVLAIALWSDATIYKLVVN
                     TYKVTLVAAFVPLCAGLFWKKATSWGALCAIVTGLIVWLGLEFFGSDNPVWPAQLVGL
                     LMATVGMVVGSLLRPDQKVRA"
     gene            complement(46797..47162)
                     /locus_tag="VLD60_06550"
     CDS             complement(46797..47162)
                     /locus_tag="VLD60_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44651.1"
                     /translation="MNRATVQLSTAVIALAFGLTTFAQEPAPAPTAPPTSIPAPAPAP
                     APMPAPSSGEMKQEGEKKMGGERHRDKAERKAEKERRKAERKAEKEKRKAEKKARKHG
                     RDKEKDGNKDDLDKNDGKN"
     gene            47471..48145
                     /locus_tag="VLD60_06555"
     CDS             47471..48145
                     /locus_tag="VLD60_06555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSB44652.1"
                     /translation="MRTLIAIVLGLFFIGMGTSATAADPTTDDQKTLYALGLAISQSL
                     GAFTLNESELEMVKTGITDGVLKKPQKVDLQAFGPKIQQLQQARIGALAEGEKKAGTA
                     YMAKAAAEAGTKKTESGILITTLKEGKGPVPKATDTVKVHYHGTLVDGTVFDSSVKRG
                     EPATFALNQVIKCWTEGVQQMKVGSKSKMVCPSSLAYGDRGAPPNIKPGATLIFEVEL
                     LEIVKQ"
     gene            48336..50153
                     /locus_tag="VLD60_06560"
     CDS             48336..50153
                     /locus_tag="VLD60_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSB44653.1"
                     /translation="MPPTILLSCESISKSYGVKPLFRDLSLALCEGDHVGLVGPNGSG
                     KSTLLKILAGLEDPDDGTRSARRQLRIGYVPQESVFPDGHTVEEVLAQVLIDEGLNPH
                     EHGGRIAKALSLGEFTRADESTGVLSGGWKKRLAIARSLMMEPDVLLMDEPTNHLDLE
                     GILWLERLLRNESQAFLVVSHDRRFLESIATRMLELNRCYPHGVFEAKGRYSDFLEQR
                     DAALAAQADYQASLANRVRREVEWLRRGPKARTTKAKSRIDTAGQLIDELQEMESRQK
                     QATAGIDFSSSGRKSKQLIVTTGLSFSLGGKPIVSGLDLRLGPGQRLGLLGPNGSGKT
                     TLLKLLAGQLKPDSGSVMHADKLRIVTFEQHRESLDQQATLRRALAPAGDAVVYQDRS
                     VHIASWAKRFLFRPEQLDLPVSRLSGGEQARLLIARLMLQPTDVLILDEPTNDLDIPT
                     LDVLEDSLLEFSGALVLVTHDRWLLDRVSTTLLALDGQGHAEWFADYAQWEAAQTRKT
                     AGKPSAASNSGDAVDSRPMPKRKGLSYQDQREWDKIEGNIVKAEAALAACRAAVNDPS
                     IVSDADALRTRYAALESAQDVVEQLYTRWAELEEKRAKV"
     gene            complement(50179..50826)
                     /locus_tag="VLD60_06565"
     CDS             complement(50179..50826)
                     /locus_tag="VLD60_06565"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44654.1"
                     /translation="MRTRQVVTTLSLASLLTMLGWTVTTAEPSFEQTARYILEVVKAF
                     RTAYVLQVVEHTRGGGTSPQEDWQKDAHFLPLPAQFVKAAAEQVESFDIGLIGLIPLN
                     PANRPKTAAETDALIQLEKDRQRRFVSFMDGDQFKAVSADLALVQSCVDCHNQHPRAA
                     RKNFQRWDVMGALVVRFKRDLDTEGFSLPAEPQKRPPGTLERLTPPPTTAPPWIR"
     gene            51070..52317
                     /locus_tag="VLD60_06570"
     CDS             51070..52317
                     /locus_tag="VLD60_06570"
                     /inference="COORDINATES: protein motif:HMM:NF023301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HSB44655.1"
                     /translation="MHSLTRPAAFLAIFTLTLTSGCETTDRLLTGAERAVTSRTGQTV
                     IDLAAGKDPAQIAKRRLDQYTRDPEAFLRDLRAAQRDFQALYDALSGKVGEKWGKKEI
                     KLPEQKKYVKYTQNYRSRAIVDFDKGEILVETVDNQAPRRSLKNAVVTTLLTPQDPRA
                     VDLFSDKEIVLTGDKEPYLLGLVLDQQGKPIRSPNEAEPFAEHLLDNKFGTREVDQDG
                     TKKTAAFVTIKMAANLPQRQAEKYRPMVQQFAQQYRISPSLVFAIIRTESNFNPYAVS
                     SAPAYGLMQLVPTSGGREAYRRAKGKDIAPSRDYLFDPENNVELGTAYLNVLTFTQLD
                     EVANQVSREYCVISAYNTGPRNVLRAFAKEIEAALNMINSLQPAAVYDQLRHHLPYQE
                     TRTYLEKVTTFRKAFISAANSSN"
     gene            complement(52326..52727)
                     /locus_tag="VLD60_06575"
     CDS             complement(52326..52727)
                     /locus_tag="VLD60_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44656.1"
                     /translation="MLRPIIRWSLAVSLCGGVLSGALTGFAQTSSPPPPSLQIQPAPA
                     PQVGPAANVSGVSSSATPAGPVATPVPGVGTIPSSSLGKSFGSAGRGLPGMPGGPPVS
                     GTPGGQDPSSTWMRPLQIPPLLCDPATDIPC"
     gene            complement(52745..53968)
                     /locus_tag="VLD60_06580"
     CDS             complement(52745..53968)
                     /locus_tag="VLD60_06580"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB44657.1"
                     /translation="MGTLTASVRCCIAGGGPAGMMLGFLLARAGVSVLVLEKHADFLR
                     DFRGDTLHPSTLEVMHELGLLDRFLALPHQKVPRLNARFGDLAITVADFSTLPTRCGF
                     VAFMPQWDFLNFLAEEGRRYPTFQLRMNTDVTDLIEEAGTIVGLRADTVDGPLDVRAD
                     LVVGADGRHSVVRARAGLSIDEFGAPMDVLWFRLSRQPGDPVDPMGRFDAGRIFIMLN
                     RGEYWQCGFVIAKGSRDQLEAKGLPAFRENVAALAPFARDRVGELHEWGPIKLLTVQV
                     DRLRRWYRAGLLCIGDAAHAMSPVGGVGINLAIQDAVAAANILAGPLYERNLATEHLQ
                     RVQVRREWPTKMTQRVQLMVQNRVIKTTLGSTGPVMPPLAFKIMAHLPVLRRIPARMI
                     GLGFRPEHVRTIVAS"
     gene            complement(54113..54241)
                     /locus_tag="VLD60_06585"
     CDS             complement(54113..54241)
                     /locus_tag="VLD60_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44658.1"
                     /translation="MIHPILGRLVPVKGTAGYIVISSFETLGYEGGVSNQVSETIS"
     gene            complement(54238..54834)
                     /gene="sigZ"
                     /locus_tag="VLD60_06590"
     CDS             complement(54238..54834)
                     /gene="sigZ"
                     /locus_tag="VLD60_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HSB44659.1"
                     /translation="MAQDLTEIWREVHDSLRGFIAKRMRNEVEADDILQEVFLRVHRR
                     VDRLNDPDRVMAWLYQITRNAIVDYYRSPDRRRELPVGLAGDVETTVPVSLPSDDESG
                     QLRTELAACLQPMLDRLSKDYREAVMMVELQGLTQEAAARRLGLSLSGMKSRVQRGRK
                     QLKQMLDECCLIQLDRRGGVADYEIRDAGCDSCRGSET"
     gene            complement(54875..55321)
                     /locus_tag="VLD60_06595"
     CDS             complement(54875..55321)
                     /locus_tag="VLD60_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HSB44660.1"
                     /translation="MRPHISLDVRSVPRSVEFYAEVFGLSPQKQTADYAKFDLAAPAL
                     NLSLVSSTGRISSVNHFGIEVDSIEEIRSWKQRLQEHGLLERVEENEVCCFARQDKLW
                     FTDPDGNAWEIFTVHEQLPVHGPLSSSGCCVPKHAGAAGPAACVAS"
     gene            complement(55553..56176)
                     /locus_tag="VLD60_06600"
     CDS             complement(55553..56176)
                     /locus_tag="VLD60_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB44661.1"
                     /translation="MNILLVDDHAVVRVGLKQLLADAFDDVKITEAGTGHDAIRHVQE
                     APWDLLILDINLPDTNGLDVLKKVKAIRPALPVVVLSFHPEEQYALRALKGGAAAYVT
                     KDSASHELATAVKTVLAGRQYVTPSLAERLEATPAQVAGRPLHTLLSDRELEVLSMIG
                     SGKSVTEIAEALDLSVKTVSTYRMRLLSKLQLKTTSALIRYALTHQL"
     gene            complement(56383..57009)
                     /locus_tag="VLD60_06605"
     CDS             complement(56383..57009)
                     /locus_tag="VLD60_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB44662.1"
                     /translation="MNMVIVDDHAIVRIGLKQLVQDAYPYATIWEAGSGREALMLLDK
                     VRWDLAILDVNLPDQNGVELLKAIKLRQPLLPVMMLSLHPEEQYALRALKAGAAAYLT
                     KERAPEELVTAIKQVLAGRKFITATLAERLATVAFSPVEQLPHERLSDREFQVLCAIG
                     KGKTVSAIAGDLSLSVKTVSTYRTRLLDKLHMKNTTELIRYTLDHTLV"
     gene            complement(57042..>59831)
                     /locus_tag="VLD60_06610"
     CDS             complement(57042..>59831)
                     /locus_tag="VLD60_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB44663.1"
                     /translation="MRLALFAVDHAGDGIFWADMKQRLIYANDAARRSLGYNSDEIKA
                     LTIADIVPHHDPERYAQCLVLLRQGQVVNYESQHRRKDGTIFPVEVSLNYLEHAGRGY
                     TCAIVRDITERKAAETALQQAHEELERRVKERTAALRESQERLELILDSTGVATWDWD
                     VTTGAVQYNERWATNRGMSFDEVIPHVSSWSDGVHPEDRDHVMQALENSLEGKIEPFE
                     AEMRVKTAAGEWTWIMGRGRVIKRDAQGKPLRMAGIEIDITARKRAERELKEAQAFVL
                     SVLENIPNMLFVKEAKDLRFVELNKAGEDLLGYHRNELIGKTDYDFFPREEADFFTRK
                     DREVLESGRLKDIPREPIQTRYRGTRILHTKKIPLYDQSGKPRYLLGISEDITERQMM
                     EEALRASEERYSSLVSQATDVIYTAGMDGRFTYINAAACAVMGYREEELLGKHYLELI
                     RPDAREAAQRFYKQQVAERIPSTYLEFPAITKDGREVWFGQRVQLRVAEGRIIGVEAI
                     TRDITARKLAEQALEEGAKCAAFAAEVSLLLNRDEPLDSQLQRCTDAAVKHLGAAFTR
                     IWLREPGDLCAECHKASWCQDRTECLHLHASSGLSTNLNGEYRRIPMGALKVGRIAQG
                     AGALFTNDVAQDERLPNKGWLKDNGLRSFAGFPLIVEDQVYGVFATFGREEISEAMRQ
                     TMESVCNGLATAIARKRAETERQAAYDRLREVTRQLAQAEEGERRRIARELHDEFGQA
                     LTGLKLDVAWLAKRLTPMISESQSAHVSSKFEAMLDTVDGLIRSVRETAAALRPGVLD
                     DLGLVAAIEWLVSSFRERTGLPCELTVDPGIQRLSLEGELSTTVFRSAQELLTNVMRH
                     AQASTVSVELSMAASQLILRVCDDGRGIQDCEWNGTHSLGLRGLYERVKSVGGTVTII
                     GAPGAGTEATLIFPL"
BASE COUNT        13077 a        18238 c        16258 g        12258 t
ORIGIN      
        1 ccattattcc tcaatatggt tgcggacacc ttttttctcc catccgctcc gctatccaac
       61 cgagctacgg gcatttgcct gtcttctgtt gttttcgtaa actagaatca cttgtcttag
      121 gaaaggaggc ctccatgata aaggcactga gtgttctatg ctcttggttc atccttgcaa
      181 tcgcggctgg gccttcacaa ggccaagagg cacctcctgc ctcgccagct gcaccggaaa
      241 ctgccgcaaa agcgactccc gcggacggcc atgtcatcca cgtcacggca ccgcacttaa
      301 tcgatggaaa ggtacggggg ccattccatc actattgcaa gatcgtctct cctgagccct
      361 tcattgagtg ccttctctac gagacggaag accataacgc gaatctcatc ggtgtggaat
      421 acattgtcgc aaaaactgtc actcgtgacg agaaggtcct tcccaagaag gtttggaaaa
      481 aggtttggca tgatcattcc gtggagggtg tcatagggaa catcaaaata ctggatatgc
      541 ctcccgataa ggccaaggaa tttgcggaca cggttgccaa gaccgatggg atcatctttg
      601 cactatggcc cgagggcgcc aaagtgccga ccggcaaagt cagtattggc acgatggttg
      661 ggcatgccgt tcaccagtga cagggaacta taagagcccc ttggctccat cccttttagc
      721 agagcggaag cccgctgatt aagggtccgc gtgatttgtc ccggccgtgg cgggagatgc
      781 attcaatggc gctgcaggat aagacaggcc cgcgcgcggg aaacggccgg agggcgtggc
      841 ctctgccggg ggaataggtg ctgacatctt tcctcctgta ggcattcgtg cgaagtggtt
      901 gtgtttccca tgagcttctc ttaggatcgc aggcggagag tacggacata tgttccctga
      961 gaaatcacct ggccaaggaa gcctgatagt tgccgccttg atcgtcctag ggatcgtatt
     1021 tgccgggcat tggatcgtca gctacgttca gaccagcaat ccgtgggcta tccttccctg
     1081 gtatggcggc gccgcagtgt taatcatcgt gtttggggtt ctacgccgcg gaggcggcta
     1141 ggacgaatcg accaacactt ggccgggcct cggtttcggc tgatttgtaa tgtgattgtc
     1201 atatgacaca gcgatccaac ccgctctccc gccgatgaag agtcagctgg gtttgtcccg
     1261 tccgttctgc agggatgaga gacgatcaac tgagaggcgg gcgataacgc cgggcccgcc
     1321 acgcgccggg aaattcagaa tgtccccgtt ttatcctcag tcttccacgc ccacgccgat
     1381 cacgatgaga accgcatagt cctgcttcgt gcagccctcg accggaggca tcgctccccc
     1441 tggcgcaact ggaaacgccg tcttctgcac gtttgccggc gagcacaaac caccaaccgc
     1501 tgccggtgta tagcgtgcgg agcggcaggc caggtgcgtc acgtcgtaga gcgtcgcgcc
     1561 tttatcgagc tcccagcgcc tgtcgccatt cctgtcggcg ggatgaatgg tcagcacagc
     1621 cgttacgtcg cgggcacccg tgaccacgta tttgccggga gggagcggaa aggtcggctg
     1681 ctccatgatc aagccgtgtt cctccagcca ccagtccgtg ccgtcgaact tccagcgcgc
     1741 cggggcgacg ccaccggcat ccttcaaccg tttatagctg ttcagctcga cgccccgagg
     1801 acccgggtcc agaggccaca gaccccacga ttgcgcgccg ctcccggagg tcgcacctgg
     1861 atcgcccaac gccgcgatga actgtgtcga tatgcgctgg aacttcgtct gacctccgcc
     1921 tgcggcgtgg gcctggcttc gactcaaatt gaatgagggg agcacgaacg aaccgagcag
     1981 aaagagaacc aggatgagca gagaaaacgt agcacgggca gaagaaccac cctctttttt
     2041 tgtcatcttt gctccttcgt ctcgtcgccg atttgggggc tggcgcaaaa cctgaggcag
     2101 cagttcctcg atggtctcat atctccattc tcgaagtggt gagattgctc ggaatcagta
     2161 cgggtgtgga aagtaaaaag gtggaaattg cgaagctaga catgagaaga cctcagccac
     2221 ggcgtttttg tagcgtagaa aagaattaca taggctgcca gtagcagggc aagtatcgtc
     2281 gccgtccaaa aaactgatgc acaaaggctt cgagatcgct catacctctc cctcgcacga
     2341 aacatagcct tttccacgat ctggttactt ttgcaacata acaaatccgg cgtaatgatc
     2401 gcttggagca gaacgaaatg tccatagcta atggtaagcg ctggacgatg acataatcag
     2461 gggaaatgct gggcagccgg gaaaagattg aaaaaagttt tgaaggttct gccctttgta
     2521 ttttgtataa ggtggtgagc cgcctgggac tcgaacccag ggccctcgcc ttaaaagggc
     2581 gatgctctac cgtctgagct agcggctcac ctaatggagg gtgcgaattc tatcactccc
     2641 cgtcggcctg tgcctagagg agtactcgcc cgtaccgttt ccctgttcgt ggagggatct
     2701 tgagcggatt ttttagaacg atggtggcgg cgcgcttcga accggtcgaa atcatatcga
     2761 tgggacattc ggcgatatct tcgatgtact gaaggtaccg cttggcttcc gcgggaagac
     2821 ctttataggt tgtcacgccg gtcgttgaag cggaccaccc cttgaagcgc ttgtacaccg
     2881 gttcacagtc agtcaatgtt tgaaggtcag ccggcatgtc ccgatagagc tggccttcgt
     2941 agcgataacc ggtgcagacc tgcagttctt tgcaaccatc aaggacgtcc agtttggtca
     3001 tcgccagtga agtaaatccg ttcaccctcg tggcataccg aaccacgacg gcatcgaacc
     3061 agccgcaccg tctcgcccgt ccggttgtag ccccgaactc tttcccccga tcttgcagcc
     3121 cggctcccac ttcgtcggtt aattcggtcg ggaacggtcc actgccgact ctggtgctgt
     3181 agaccttggc tacaccaagc acggcatcga tcttcgtggg ccccacgccg gtcccggtcg
     3241 aggcgcctcc ggctgtcgag ctcgacgagg tgacgtacgg ataggtcccc agatccacat
     3301 caaggtgcgt cccctgcgca ccttcgaaca ggatggtctt tctggcatca atcatccggt
     3361 tcaccagcat ggttgcatca actacgtggc ttctcagtcg gtctgcgtag cccatatact
     3421 gctggtagac cttttcaact tcgaaccgct cgaccttgta cagctgttcc aagaaccagt
     3481 tgatttcgac caggttctct tccaatttct tgcgaaagac ctttggattc agcaggtcgc
     3541 ccatgcgaat cccgatgcgg gaaatcttgt cggcatagca gggaccgatg cctcgaccgg
     3601 ttgttccgat acggcgagaa cccttcgatt gttcagctgc tttatcgatg gccttgtgat
     3661 agggcaggat cacatgcgcc cgctggctga tgacaaagtt cttcccgaac ttgagcccct
     3721 ggccctggag atgatccatt tcctcgatca gggatgccgg atcgacgaca actccgttgc
     3781 caatcacaca gagcgtgccg cgatagagaa ttccggaagg aatgaggtga aagataaacg
     3841 tcccgtggtc gttaatgacg gtgtgtcccg cattcgagcc gccttgatag cggacgacgg
     3901 catcggcatc acgggccaag atatcgacga tcttgccctt gccttcgtcg ccccactgcg
     3961 cacctatgac aatcagatta cccatcgatg cgtagatccc aaaaaacagc cctggcagga
     4021 ttcaaggcca gggccgaggg gtcatggtag gggcccgtct aatgtttgtc aaggaccgtg
     4081 ctggtcggtt ctttcttcgc tcatttcttt ctttcatatt caagggcagg tcccttttct
     4141 tgactgccaa caggttgcag tgttaggatc accgcgattt cacggtgctt tttcatcgtt
     4201 ccgaagaatc agagtggggc tgtggcgcag ttgggagcgc gcgtgaatgg cattcacgag
     4261 gtcgccggtt caatcccggc cagctccacc aaaccaagct tgctcgtacc gtcggctatt
     4321 catcggttct ttgtaacggg cgccgacgga taaaacccta actgtgagct tcccttttgt
     4381 aaaccaacct tcggttgaac cgcgcgtgtt tcatcgctgt accatgattc gcgcgcgcgg
     4441 ataaacactt ccagttcatt ctcggtcagt tgatgctatt gccatgcttc aaattgagtc
     4501 aattcataag cagttttcga ccaaagtcct attcgacgat gcctccgcgc atctgcggcc
     4561 gcacacgcgt gtcgggctcg ttggaccgaa cggaacgggc aagaccacat tatttaaaat
     4621 gattctcggc gaagaatcgc ccggcgaagg tacgatccga aagcgcccgc gcctccgtat
     4681 tgggtacctt cctcaggaac tcgaaaccat cacggggaaa acggcgctcg acgctgccca
     4741 tcgagacgaa tatcccgaac acgaggcgaa gcggattctc tctggactcg ggttcagtga
     4801 agcggatttt gcgcgaccgg tcgagaacct ttccggcggc tatcgcatgc gcgtggcgct
     4861 ggcccatctg ttgctttcca atccggacgt cctcatgctg gacgagccga ccaaccattt
     4921 ggacaagccg actcagcggt ggttcgaaga gtttctgctg aattcgaaac tcaccttgct
     4981 cgtcatcagt cacgacaccg cctttctcga ccgcattgcg acccacatct gggaacttcg
     5041 cgataagaag atccaggagt tccggggaaa ctatacgaag ttccgcgaat tgcgggctga
     5101 gcgcgacgcg caacttcaag ccgcggccaa tcggcaggcc aaggaagtgg cgcgggtcca
     5161 acaatttgtc gaccgctttc gttatcaggc caacaaggcc cgccaggtgc agtcgcgcat
     5221 caagcagctg gagaaggtca aattgattga gctccagcgt gataccaagc gggtgaagtt
     5281 caagtttccc ctcccctcaa cgagcggacg ccacgtcctg gaactcaagg gggtgagcaa
     5341 gcgatacggc gagaaggtcg tctattcatc gctggacttt tccgtcgagc gcggccagag
     5401 aatcgccctc gtcggtgaaa acggcgcggg gaaaagtacg ctgctcaaga tgctggccgg
     5461 cgttctggca ccggacagtg gcacaagaac ggtcggccac ggcgtcaccc tccattattt
     5521 cgctcaacac caggcggaaa cgctcaatcc tgaacacacg attctggaat cattagcgga
     5581 agtctcaacc acggcggaga cgaatttcct tcgcgggctt gccggcgcgt tcctattctc
     5641 cggtcccgac cagaagaaac cgatcaaagc gctcagcggc ggcgaacgga accgcgtcgc
     5701 actggcgaga atgttggtcg agccggccaa tacgctgttg ctcgacgagc cgaccaatca
     5761 cctggaccct gcctcggtcg atgtgctgac cgacgccctc acggagtttc ccggcacgat
     5821 catctttatt tcgcacgacc ctacgttcct gacgcgcatc tccacgcgca ttgtggaaat
     5881 tgaggagggc aacgccagga acttcttcgg cgactacgaa tactatttgt ggaaaaaggc
     5941 tcaagagttc gaatcgatca aggaatccag cgaggagttg atcggaaaac agccggtgaa
     6001 gtcgtcgggg ccaacgaagg caatggccgt ccagagccag cccaaatcaa cgtccggaga
     6061 ccgtcgtgac ttgaacaaga cacaggctcg attggaaaaa caggtcgcac gagcggaaag
     6121 cgagatcgcg gagcatgagt ccaagattaa ggagcgcgat cagatcctgg cagaccctgg
     6181 gctctacaaa gagtttgcga aatggaatgg gctgcatcag gaacaagaaa tctggaagaa
     6241 agagcttgaa cgcttgaccg cgcggtggga atcgttatcg gcagaactca cagacgtgaa
     6301 acagaaactc gaagccttcc gttaggagga agatttaatc gtttcttcga gatagtaata
     6361 cagccatttg tttttctctt tcgtcaccac ttcatcccat acgaccccga tcagaaatcc
     6421 cttctcccag ggcttgaccc acgccacagt cccatcaagt ttctcctgct gctccgcacg
     6481 atccgcatcg agaaacgcga gagacacagt taccttctca gccatcacca gcttttcttt
     6541 ggcgtgcaaa ccggcaccga ccttgttcac attatccagc acggctgtga tggccttggc
     6601 gccgctcttg ggagacacca atgccgatcc gaccaatgtg gcccgcacaa gtctgcgccg
     6661 ttcgttgctg gccaccatgg ctggagtggg tttcgactcc ggacgtttca tgggtctaag
     6721 tataaccgat tagaaatctt tgtccatcgg gaagaaatgc acctatgctc ccttcggccg
     6781 gtagtatttt cggcccttta aagcctccgg gagatgctcc tgctccttgc tggcctgcgg
     6841 atcatcgtgc acgtagcggt atcccttccc ataatccata tcgcgcatca tggaggtcac
     6901 ggcatttcga agatgcagtg gaacccctag atttccatgg gttttgacat cttttatggc
     6961 ttcaagcagc ccaacgtagg aggcattgtc cttcggtctc gttgcgaggt aggtcgttcc
     7021 atgggccaga ttgatctgac cttccgggag tccgacaaac tgcacagctt gggccacggc
     7081 gtttgccacg agcaagccgg aagggtcggc attgccgata tcctccgacg caaaaatcac
     7141 catacgccgg gcgatgaatt ttgggtcttc tccagcttcc agcatcctcg cgagccaata
     7201 gagcgcgccg ttcggatcgg aatcgcgcag gctcttaata taggcggaga tgacgttgta
     7261 atgctcttcg cctgacttat cgtagcgaag agatttccgc gccagcgccg catcgaccgc
     7321 tatgccatcg attgctcgta cgccgtccgc attaaccggc gcttgcgtca caacgaactc
     7381 caacgccgtc agcagcgccc tcgcatcgcc gtttccgaat gcaatgagtc gctgccgcgc
     7441 ctccggtgat agccgggcat tccatttccc taatccaatg gcagcatccg agagcgctcg
     7501 atcgagaata tggcccaatg cctcatcaga aagtggcttg aggacgacga ccagtgaacg
     7561 cgacaggagc gggctgatga cctcgaatga cgggttctcg gtcgttgcgc caaccaggat
     7621 gaccgtgccc cgctcgacat gcgggagaaa cgcatcctgc tgcgctttgt tgaatcgatg
     7681 gatttcgtcg acgaataaaa tggtccgttg accgttgatc gcctgacgtt gctcggccgc
     7741 tttgatgatc gtccgcaact ccgggactcc actcgtcacg gcggagaacg ggacgaactg
     7801 cgccttcgtg tagcgggcga tgaggtgcgc cagagtggtc ttgcccgagc cgggcggtcc
     7861 ccagaaaatc acggaagaaa gtcgatcggt ctcgatggcg cgccgcaagg gccgatccgg
     7921 ggcagtgatc tcctcttgcc cgacgaaatc ggcgaactcg caaggccgca tccgctcggc
     7981 taacggcgct gtaacgcttc gtgctcgttc gggggatgaa aacaggtcag gtgcctggtc
     8041 gtgctgtgcc gacatcgact tgcctcgggg attgccggga ttttatacgc ccgtcgaacg
     8101 gatcgcaaac gccccttgac cataagcgcc gccgatgtta cgaacagccc taagccacgg
     8161 aggagattgt gcaggccagg atcaacggca tttcgctcgc ctacagcgac aagggaacag
     8221 gccttcctat cgtttttctc cacgctttcc ccttgaatcg aaccatgtgg gcacagcagg
     8281 aagccgtcct ttcagtgaag tgccgggtca tcacgatcga tctgcgaggg cacggtgaat
     8341 ccgacgcccc gctctggcac tacacgcttg aacaagcagc cgacgatgtc cgggcgttat
     8401 tggatcagct ctcgatccag caagcggtgt tcgtgggact ctcgatgggc ggttacatcc
     8461 tcttggcgtt ctatcgaaaa tatgccgagc gcgtgaaggg tctggtttta gccgatacga
     8521 gggcacaggc agacacagcg gaagggaaag agggtcgctt tcagatggcg cagacggcct
     8581 ataaaaaggg accggctgcc atcgcagaca tcatgattcc aaaactgctc agtcctgtga
     8641 ccgtccaaac gagaacggaa atcgttcaga acgtgagagc catgatcgaa agcaaccaga
     8701 tcagcggtat tgccggagac ttaatggcaa tggctgagcg acaggattca gttccactct
     8761 tgaaacagat cgcctgccct actcaaatca tcgtcggcgg cctggaccaa gccaccccgc
     8821 cctcggatgc caggctgatg gccgaacaaa ttccaggggc tcgattggcg ttgattccga
     8881 acgccgccca cctggccaat ctcgaacagc cagaatcctt caatcagatc ctggcttctt
     8941 ttgtgtcctc tctcgcggac cgctaatcgg agtcgccgac gctgccccgg cggatcagct
     9001 tcaaaaattc caggcgggtc tgttgctggg tacgaaaggc tccgagcatc gaactgctga
     9061 ccgctacggt gttctgtttt tccactccgc gcatcatcat gcagaggtgt cgcgcttcca
     9121 cgacgacgcc caccccattc gcgttgagtt tcgactggag cgtttcggcg atctggaccg
     9181 tcagccgttc ctgcacttga agccgtctgg caaacgtgtc cacgatgcgc gggatcttgc
     9241 tcaggcccac gaccttctta ttgggcaggt agcccacgtg gaccttgccg aagaatggca
     9301 acaagtggtg ctcgcacatg ctgaagaaat cgatgtcttt cacgatcacc atctcatcgt
     9361 actcgatcgg aaagagggcc ccgttcaaca gatggtcgac gttacgctgg tagccctgcg
     9421 tcataaaggc aaacgccttg gcgacccgtt cgggtgtttt aatcagaccg ttgcggcccg
     9481 gcttctcgcc gagagcaagc aacatctcgg tcaccaatga ctgcaagatc tccaaatcgg
     9541 gcggtctccc gctgccgttc acgtcttcga cctgccgccg ccgtccctgt gcccttcccc
     9601 gcttagccat gcaatccccc tatacactgc gccgctcctg catattcaaa gtaattgtct
     9661 ctggtctcga tgacgccaac cttttccaac tgcccggccg gtagttgggt caccaacaaa
     9721 tcccagatca gtttcacgag attctccccg gtcgtcgtgt attccgcgaa ggccggatcc
     9781 tggttcaagt cgtgatggtc aaaccgcttc acgatggcgc cctcgaccac gcgatcaagc
     9841 cgttcaagat cgcgagcctc ctgactccca ccgctcttga ccgtgaccag caccacgtaa
     9901 ttgtggccat gtccgttggg attattgcat ttcccgaagg ccgcccagtt ttcctcgggc
     9961 gtgagataat cggtgtgcag tctgtgcgcg gcgcaaaacc gatagcgcct ggtgacggtg
    10021 acttgtgcca tggatgcccg cagctcgtga aaagagagaa gccaggatca cccgttcatc
    10081 cgagtaaccc tggcttcgtc attcaaaccc tattgtcggc gctcgtccgg caagcccgta
    10141 atgccgctta tgcgctgaat gagctaccgc agccgcacgt cgtcttggct tgcggattct
    10201 taatggagaa tccggagccc tgcacgctgt cgacataatc gacttcggcg ccctggagca
    10261 gtggcgcgct ctgggagtcc atgatcacct tgacgtcgcc cttctcgatg acggtgtcgt
    10321 cgtcggccat cttggactcg aatgccattc catattgata cccatgacag ccgccgccgc
    10381 ggacatagat ccgcaacccg acgacatcct tttcttcctt catcaactcc tggatcttct
    10441 gttctgccac tgcggtgatc gtaaccatcg tcgtcctccc tttcgtcaga ccggccttct
    10501 gaacctgcct ggatttcgtt agtttaacac ctctttaccg aatatcaaca ccctgcgcct
    10561 ctgatttttc cgtggttggc gccgcccact tcgtctccgg gaccctcacc accacatcgc
    10621 ccaagacctt cgcctggcat ccaagtctgt gccaggcctc gctgagagcc tctcgatcca
    10681 gtagatcctg ctcttcgaaa tcgatctccg acagatgctc ggcgccgctt tgcacttcaa
    10741 ctcgacatgt ggtgcaggag gcgttgcctc cgcaatcgtg gttgagagga aaccccaacg
    10801 ctttcgccgc atccagcaag gtggtgttcc ggggaacgac tccgctcttg ccctgcggat
    10861 gcagaaatgt cacgcgcggc atccctatgc cctcttaatg atgcgcaacg gcctcgaccg
    10921 gtaccgccgg gtatggacag cgctgtagtc gtatgtgctc gtcgaactcg tggcggaaga
    10981 gcttcacgct gctctgcacc agcaccgagg cgccggtcac gagcgtgcag tacccggttc
    11041 ccttgacgaa tccgcagagc tgcaacaggc tgtcgagatc cttctgcgtc ccctcgccgg
    11101 cttcgatctt ggccatcagc gtcgccagat tgatcgtgcc catgcggcaa ggcggacatt
    11161 gcccgcaact ctcgcctttg aagaagttgg agaatttcag ggtctgccgc accatacagg
    11221 tcgcatcgtc gacgacaatg acccctgctg agcccaaccc cgttcccgcc ttcttcaacg
    11281 agtcaaaatc cattggcaaa tccagctgat cggcggtaat catcgaaaac gccggtcccc
    11341 cagggaatac cgcttttatc ttccggccac ccggcacgcc gcccccacac gtatcaatca
    11401 gttctcgaac agtcaccccc atcggcattt catacacgcc gggacggttt atcgagccgc
    11461 ttaaggaaaa catcatggtg ccgggacatt tctctgtacc gacttgtgtg aaccaggccg
    11521 ctcctttatg gatgatacga gggatattac agagcgtctc gacgttattc accagtgtcg
    11581 gtttgccgta gagaccgtaa tccgtcggat agaacggagg tttctgtcgc ggcatggcag
    11641 gacgcccttg catcgactcc aacatcgccg tctcttctcc cgccacataa ctcccatgcc
    11701 cttcgaacac ttcgagatcg atatcgatcc cgctccccat gatattcttc ccgatgaatc
    11761 cccgctcctt cgcctgagcc aaggctttct tcaggttctc gcgctcttcg tgatattcgt
    11821 gattgacata gataaacgac gctttggcct gtgccgtatg agaagcaatc aggcagcctt
    11881 cgatcagttg atggggaatc gttttcagca aatggcgatc tttaaacgtc ccaggctcgt
    11941 gctcgccggc gttacacaca aaatagcgct ctttgacccg atggttcagg accttgtccc
    12001 acttcacgcc agtaggaaat cccgcgccgc cgcgaccacg aagaccggag gtcttgagtt
    12061 cttgaacgac ctgatccggt gagagctcct tcacacattt tttccatgct tcatagcccc
    12121 cgaccttgat gtaggcatca atgtcccagg ggctgccctc gagcttctga acgagacgtg
    12181 gctcaaccaa tgtcgtcatg ccgatcctgt gctccgacct gaggatagag gtgagtggct
    12241 actatacgag ccccttcgcc cctccgtcaa ggtaaaggca tcagaatagg cctgaatctc
    12301 aatgagttag gaccacagcc ctattccaga ctgggcatat tggccatgga ggaaatgaaa
    12361 tcgtggagcg tgagaaaagt ccgggccggt agggagcccc accggcccgt gaaatcgact
    12421 acttgaagtg gctgccggct cccatgaaga acaacatcgg gatcgacaac atgaaattca
    12481 cgcgagaggc cagtaacgcg gtccggcccc attgagccat ttcagccggc gccggagtcc
    12541 cctgcgctgc ggcagaaacc gccgcgataa tcttcttttg attcggccag atgatcgcat
    12601 gaacattggc catcatgatg atgcccaaca agcctccgat cccgagtgcc atggctccgg
    12661 ttccgccctt ggaatacata tagaagtaca gcgcgatgcc ggccagtacc gtaaccgtgg
    12721 cgccatggcg aaaccagttg agcgccgccg gcatgagctt aggaatgacc acgttcttcg
    12781 tcgggccatc caaactcttg aggaatgccg cattgatcag gttgaaaaag tacagcaacc
    12841 cgatccacgt aatgcctgcc aagaaatgga cccagcggaa gaggatgccg acccagtcgg
    12901 cctcaccggc gcccaccccg gacatgccaa gaaacaacac aaccaacacc agggcaagag
    12961 caaacccggc ccccattgtc tgcatcggat cttcaagaaa tttcatagcg tctcctcatt
    13021 cagtgcggtg ctaagctatt ttctgtactg tagcccctct ctattgtcaa gcgaagggct
    13081 cacgccatca accggtcgac cgctgtgcag agctctcgca ccgcgttggc ggatacttcg
    13141 agagcggtcc gctcctcagt cgtgagttca tattccacta cctgctcggc gccgccgcgt
    13201 ccgatcttca caggtacccc aacgacgaca ttgtgcaatc catattcgcc ctcgcagaac
    13261 accgcgcagg gcaacacccg cttctgatct ttatgaatcg cctcgaccat atccaccgcc
    13321 gaagccgacg gggcgtagaa agcactgccc gttttgagca agccgacaat ctcggctccg
    13381 ccgtcgcgcg tccgcttgac gatcgcgtcc agcttttcct tcgacatcaa ttccgacacc
    13441 ggcttccctt tcaccgtcgt gtaccgcggg agcggcacca tggtgtcgcc atgtcccccc
    13501 agcaccatgg cctgcacatc gggaccagcc accttcaacg catccgcaat gaaggtccgc
    13561 atcctggccg aatccaatac accggccata ccgataattt tagttttcgg caaaccgctg
    13621 acctttctgg ccacgtgtac catcgcgtcc agaggattcg tcacgaggat caggatgatc
    13681 tcgggagatc gggacaccaa ttcggtgacg accgatctca cgattttggc gttcgtgaca
    13741 agcagctcat cccggctcat gcccggcttt ctcggaatgc cggaggtaat gaccgcgacg
    13801 gaggacccag ccgtttccgc atacccgttg gtccctacga cccttgtact atagccgcac
    13861 acaggaccgg cctgcgaaat gtccagggcc tttccttgtg gcacaccctc gacgatatcg
    13921 accagcacaa cgtcataggt gtccttctcc gccagccgtt gtgccgtcgt cccaccgaca
    13981 tttcccgctc cgactaccgt gatcttcggt ctcgccatac ttcctccgga gtttctaagt
    14041 cctgagtgaa cggtcccgga atttcaatct tccgggcctc ggcattcagc actgctctct
    14101 cagcattagt cacgcgtgtt cgtgttccgt ccaccccgcc actttaaaat tgatcgtgca
    14161 aacaaagttc tccccgtcgt agaagttgac cttgatcttt ttctttccgt atgtttttgc
    14221 cagaaacgct tccgggttgt cgaccagttc ctggaagccg ccaggaggga actcgccaag
    14281 atcgtgaaag acgacgatca tcccggcctt cacttcctgg agaatctttg cggagcagcg
    14341 cggatagacc tcccaaaatt tggcctcgac catctccttc gtcggctcct gtcgatcttc
    14401 accgggcttc acgggctgcg cgaattttgc cagcctccgg acatagggat attgatgccc
    14461 ggtaaacaac ttgtacaacc agggtggaac cgtcggtctc tctgctgaat ttgccatcga
    14521 tgctccgtac gcctggcgtt gctctcgacc gatgcccctg ctacgtggtc aactgtcgat
    14581 agatccgcgg aagtttcgcc gggagcgcct cgacccgatc gatcaccgca tactgcacgt
    14641 cgccatacat gcggcgcagg tagctgtccg cttcgtggtc gattgtcaca caaaacgggt
    14701 cgatgccctg ccgccgcgct tctcgcaggg cggctttggt atcctccaac gaatattcgt
    14761 ctttgtagtc gccgtcgagc ggtcgcccat cgctcaacag gatcagaatt ttgattctgg
    14821 cttcgcggga tttgagtttg gaaatcgcat gacgaatggc cgcgccgtcc cgattctgct
    14881 ggcgaggacc aagaccgccc aagcggtgag cagcggcagg acccaatcgc tcgtcgaaat
    14941 ccttgaccgt caagaaatcc accgatgcac gcccctggcc cgaataggcg tagagaccga
    15001 actgatcgcc gatcgcatcg agcgcttcac agaggagcac gagactttct ttctcgacat
    15061 cgatcacccg cctgccattt tcgacctggc ggctcgtcga tccgctcaca tcgaccagga
    15121 aggccacagc cacgtcgcgc tccttctttt catgacgaac gtagacgcgg tcgctgccct
    15181 cgaaaccggc gcgctgttcc gtggcccgcc gcaccaccgc gtcgatatcc acatcctcgc
    15241 catccgcctg tcctgctacg cggcgaaatg ccgggggacg caggccctcg aaaaaacggc
    15301 gaagtgactt gatctcgctc cgatgagccg acagcgccgc agatacgctg tcgtcagagc
    15361 cggcatccgc ggatcgctcg ataacccggc accagttcat ccggtaatcc tgaatcgaac
    15421 agtcccactc gggataacga acagctcgtt cgcctccgcc ggtcgattca gaaacaggct
    15481 gctgctccac atcaagagcg agcaattcct ctacaaccga cggcagggac cgtcctcctc
    15541 ccagcatgtc accggagata gattcctgtt cgtcgcgacg gctaccctga ccagcgccgg
    15601 atcgctcctt cgaaccgcca ccttggctgg ccatgcgatc gaggtcggcg tgcgactctt
    15661 cggcctgctc ctgatcccgc ttgatgaact caggattcat ttctccgcga tagacccagt
    15721 tggtgacggg acgatagacc tcgctctggt tctccgaagc cgtcggcccc acaccgagtt
    15781 cttgcgcgtc ttccttggcc tgcgccgctg tgatcatttc agcgcgcggc gctaacaact
    15841 cttccatccg aacatagatg gtatcgacga gacgaaccgt cttttccgct gtcgctgtgg
    15901 tcgcgaagac aggctgacac aggttccaga ggatcaagac ttcttctatg acggcatgag
    15961 ggactgccgt cgcttccgac tctccggtgg acaaccgcaa gaggcaatcg acaatcagtt
    16021 ccttggcagt taaaccgtgg gcggggtcac gcggggtgat ggcttcagca gccaaccgcg
    16081 cgaggtcgcg ccggattcca ggatactctg cctgaagcaa aaattcgatg cgtgcatcct
    16141 ccaacaccgc ccagagatcg tggatcaagc gtggatgagg gtagagccga aatagtgcgg
    16201 ccagtgaatc cggtctggtc tgctgagaac ggccatagcg tcgacatacc gtctcgacca
    16261 gatccgtaag cggctccatc gtcaggcggt aggtgccgaa ctccaggtga ccggcttcat
    16321 gcgctgccat gattagaaag agacgttcgt tttcttccgc ggtcggatag cgacgaagga
    16381 gagcaggtag tgcaatcgtc cgtccattgg cgctcaccat cgcgcgggtt gcgagagccg
    16441 tacctgattc cgggagcgcg gtaatggata cgtccgttcc gcagagccct tggacaaaga
    16501 gcttgatctt gcgggccact tgcctgagcg ggacgccgct cagcgcctgt tcaaccgacg
    16561 agagggcctt ctgcgactcc atcgcaaaat aggcccgcgc accctcagga ctataggcca
    16621 gcacttccat gccagcttta aaccagttct caaaacgcga cagcgcctgc gatacatcgc
    16681 cgatgaggcc gacgatctca gggcattggc gcatgtacga cagcgccgcc tgggcatctt
    16741 tctccgcaag caaggagcca tactgcaaga ctttcacttg ccactccatc gtcggcaggg
    16801 ctctgaggag gcgcggcgac tcggccagcc aggcgacgcc ggcttccgga gaacgttcag
    16861 cgagcagggc tcccaaggcc acgaccttcg agcgaaccgg tatatgttcg atatcgccga
    16921 ggatggcggg gctggttctg agatactcca ttgtagccat gtagtccggc tttccaatgg
    16981 tattcggcgc aatcagtttc atgccgaagg acaaccagtt tcgcacctcg ccgagcggaa
    17041 ggacgggagc cagcgcgggg atttggcgaa tgtattccag cccgaccacg acatcgacct
    17101 tggtcaattc cacgctgatc tcaagccacg gcttcagttg ctctgaagga acaaccgcga
    17161 gaatctgcgg agcagttctc agatactcca acgtcacatt ggcatcctca tcggcaatct
    17221 ccagcccgat cgccagaatc gacgcgcgtg catcggcctg ttcaatcaga ccaagaatca
    17281 acgagctgtc tttgaaatat ttgagcgcgg tggcaccgga ggattccgcc agtgcgacac
    17341 ccagatcaag ccaggggatg acctgcgaga gcccggcacg gcgatccagt tctggaagcg
    17401 cctcgatcgc cgcacgcgct gctttcgaag acacctcttc cagctcatcc agcaaggcca
    17461 acaatgccgc aaccctgtct gcttggcctg ccgactcagc caatcgagcg acgaccgcgt
    17521 tcgaaatcgc ggcccccaac tcgccggtca gccgatcaat caaggctttt tgtgccggta
    17581 aatccgccat ggctccttac tcaaaacgcg cgaagttgtg gattgtaaag gagcgaattc
    17641 cccttgtaaa ctaggcgatc gtatcacttg tcgtctctga gaggagcatt ttcatggccg
    17701 aacctaaaca agagagcctc gataagatgt ggaaatacgt gaagggtttt gctgaaaaaa
    17761 gcgggaccac catgcatccc aatcctgttg tcaccgaagc ggttgtgaaa ggcctcgcca
    17821 tgcacgtaga tgaactgggc aaaccgctct gtccctgcaa tttctataag gacaaagggg
    17881 tcgaagccaa actccgccgc tggatctgtg cctgcgacga gatgcagatc tataaatact
    17941 gccactgcct gttgtttgtt cgcgaggacg ggatgccgat cacggagtat ttgcccgaag
    18001 gccatgaagg tcgagaagtc tacggagtga ttacggaccc aagtcccgac aaaggcagag
    18061 ccctaaaaca caaagccata tcaacatctc ttccagctcc tgagacacaa aaacgataga
    18121 tgtctcgctt ttctcctcac tgccagccgg atacttcata tcccttttca atcaaacaac
    18181 gcacgaccgc atccgcgtag ggcggatccg gttccagcgg cttataggcc ccgccagcca
    18241 ggccgataat gagtcccagc cctcctccta ctgccgcccc gattgcagcg ccgctggcgc
    18301 cgccgatcaa gccggcagac gcaccagccg ccgcgcctcc caccaaccca agtacagcgc
    18361 cgcttgcagc attactcgtg cgattcgttc cgtgctgaag acctgccgcc tcggcctttt
    18421 cctggcaagc cactacatca agtttcgacg cttcacgccc ctcaagctgc actttcgcat
    18481 tcgaccgcaa catcggctcc ggccccgcac aggccaaaag gctcatcgtg agtgtcatga
    18541 ccaacacact cttacacttc atcatgcgct tctctttctt ttcgaaagcg ggatgggcat
    18601 tctttcctgc gggatggtga cgttaggctc cactgttcgc atgcagccag gacctgcttc
    18661 tggttctcac cctccagccc tcgcggcacc aagacggcgc gttttccgtc agatcgcgca
    18721 tggcgcgagc aaagagagca cccccactgt ctcgcgcaat cctctactgc caccccgtta
    18781 cttcataccc tttctcctgc agacagcgat tcacgaaatt cgtataggcc tggttcggct
    18841 gcgaagagcc ggctctaaac aggcccatga gcaatcccca cactgcgccg ctcgccgcgc
    18901 caatcgccga tccctgacca gctgcgccga cgatcgcgcc gcccaccgcg ccgctcgccg
    18961 caccaactcc tgcgccgacc gccgtactcg tcgccacttg tccctccttg ccgcttcctt
    19021 cctcagcacc ggaggtttca gccagctgct tgcaggcttc gatgtcctgt tccgccactt
    19081 ccttccccac cgatttgaaa tgcgtgttgg ggtagagaat aggatgagca ctggaacagg
    19141 cgttgaggag aactacccca caaacaaggt atcggagaga acgtgtcatg tagcggctgg
    19201 tcctttctcg agcagacgag ctgtcacgtg agcggcattc acgccatcca gacgcacgcg
    19261 cttatgccgt ccactcgcgc cggaatcgat gtgcacggca gagagaggaa gagacaacgt
    19321 ctgcgccaga aaccggatca actcttcatt agccgcgccg tcgacagggg gagccgcaat
    19381 tcggatcttg agcgcatcgc cgtgaattcc gacacaggct gtgcgagaag ctttcggctg
    19441 gacatggacc gtgaggacgg ctccgtcctt gctgtcctgc acgatccgag gagtcattcg
    19501 tgatagggcg gcaccggtta gaaaattgcc tttaccagtt ccatgatact gcgttgcata
    19561 tcgggatcgt cggtgatcgg cctggcaatc gccacgtcgc aggctcgttc gattgcgaca
    19621 ccctcgcgaa tcagggtgcc ggcatagatc aacagcctcg tgctcacacc ttcttccaag
    19681 ccatgattct taagattgcg caccttgccg ccgagcttca ccaggctggc ggcgacatcg
    19741 gcagcaacac ccgcttcccg ctgcacgatc gccgtttcga tgtcggggct gggataatcg
    19801 aactcgatgg ccatgaagcg ctgcttggtg ctctgtttca aatctttgag cacgctctgg
    19861 tagccgggat tgtaagaaat gaccaggagg aaatcgtcgg cggcctcgat gacttggccc
    19921 ttcttctcga tcggcaacaa acggcggtcg tcgctgaggg gatggatgat caccgtcgta
    19981 tccttgcgag cctcaaccac ttcatcgagg tagacaatcg cgccgtgttt cacgccaagc
    20041 gtcagcgggc catcgaccca gacggtctcc tgtcccttca acagatagcg tccaaccaga
    20101 tccgatgccg tgaggtcttc gtgacaggcc acggtaatca acggacgtcc cagtcggtag
    20161 gccatgtact gcacgaagcg cgtcttgccg cagccggtgg ggcccttgag catgacgggc
    20221 atccggttct tcgccgccgt cgtaaacaaa tcgacttccc cgcggacctc ggcatagaac
    20281 ggttcttgtt ggatacgata gtgcccgatg tcgatctcgc gtcccttgtc agtcatgttc
    20341 tcgtttccag tcgcactgca tcgtgaccgt catcatgttc ccactgtccg ggcacgataa
    20401 acggaacggc ggctgaagat caagccggga gcgagacccg taacccatga tcaggccact
    20461 tgcacaccgt ccagcacgtc gcgcactttc ttcgcgagca cgttcggagt aaagggtttg
    20521 ggaaggaacg acgatgggcg caaaattcct cctcgctcga atgccggatg gtccgggtag
    20581 ccggacatgt agagcacctt gatgcccggc ctgacgactt gaattttctc cgccacctcg
    20641 ggaccgctca tctgcggcat gacgacgtcc gtgagtaaca aatgaatggg cccctgatgc
    20701 ttcgccccgg tcataagtgc ctcgatccca tggcgggcct ccagcaccgt gtagccatgg
    20761 aggcgcaagg tttcttggac caggccgcgc acggccggtt cgtcttcgac caacaggatc
    20821 gtttctcggc cgtatggccg ctccgtgata tcctgctgtc cgcctctgtt ttgcgcgccc
    20881 tgctcaatca gcgggaaata gattttaaag gtcgcgccct gccccggctt actctcaaca
    20941 acgatgcttc cgccgttctg cttgacgatt ccatacacgg tggacagccc caatcctgtc
    21001 cctttgcctt tttccttggt tgtgaaaaat ggttcaaaga gatgggattg cgtctcttcg
    21061 tccatcccgt ggccggtatc gcgaaccgcc agcaacacat aggctccggg cttcacgacg
    21121 ctgggattct ggcgcgtcgg gttcccgacc gtaacgttgt gggtttcaat cgtaagccgc
    21181 ccgccctttg gcatcgcatc gcgggcattg accgcgagat tcatgatgac ctgctcgatc
    21241 tgcccaggat cagccttgat ggccccggcg tgcggagtca actcggtgca gagttcaacg
    21301 atgtcctctc ccaacagacg gcgtaacata ccgtccatat tggcgatgag agcattgagg
    21361 tcgatcactt tgggtgcgat aaactgacgg cggctgaagg cgagcaactg gcgggtcaga
    21421 ccggaagcgc gatctgcagc cttcttcacc tcttccatgt ccttacgcat gggatcgtct
    21481 tgacttaaac ggcccagcag aagttcggtg tatccgcgaa tcaccgtgag cagattattg
    21541 aaatcgtgcg ccaccccgcc ggctaaccgg ccaaccgctt ccattttctg cgactgccgt
    21601 aactgtgttt ctgtctgctg cagcgcctcc tcggcccgcg cacgatcggc gaattgcccg
    21661 agtttcaaaa cgacgtcgtc gatcatcttc aacagctgtt catcgggcac acgaacctgg
    21721 cggctgaaca attcgatgac gccctcgacc tcgccaccga ctcggatcgg aaatccaaag
    21781 gcgccgtgga acccaatctc acgagcttgc tttcgtcgtg agaagctttc atcctcgacc
    21841 acgtcgctga tccacgcggc ctttccgttg gcccaaatcc gtccaggcag gctctcccct
    21901 accttaaagc cccgttccca cgtgtcggtt aagaatacgt ccgccttcaa caccgcgctg
    21961 tgccactgat ccaggcagcg aagcaccccg gtggccttgt ccacccgcca aaaaattccg
    22021 cagtcccatt caaggttttg tcccaccgcc tgcatgattc tcggcaccgc ttcctccagc
    22081 gtccgcgact cagccagcac gcgtgtgacc gcatactggg ccgtcaaacg ttcgtccgcc
    22141 cgtttgcgtt cggtaatgtc ggaaaacgac acgacgacgg aggccggagt cattcggcct
    22201 tcggcagcga gcggccggga gttgatgtcg atccatcgca gttttccatc cggtctgtag
    22261 atccccatga ccgcactggc gcagggctga ccggtccgaa gagtgatcag cgccgggtac
    22321 tcttctagtg gaaacggcga gccatcttcg cggatcgtct gccagcgtcc atcgcggacg
    22381 gaacgtcccg tcaattcctc cagggacaag tccaaaattc gctcggcact cgcgttagcg
    22441 gttttgagca ttccctccgc gtcgatgacc acaacccctt cgtccagcgc attcaccacg
    22501 gagcgatacc gcagctcgct ctctttcagt ttcgtttccg cctcccgccg cacggtaatg
    22561 tctcgaatga aggcgctgaa aaaaatggtg tcgccgatgc gaaccggtga aatggcgagt
    22621 tccacgggaa attcgtttcc agagcgatgg agcgccagca cttcgatacg acggttgagg
    22681 atgtgcgtct ccccgctggc caggtagtgg cgaaggccgc gctcgtgggc ctcccgctct
    22741 ctgggaggaa tgatcaactc cgccaggcgc tgcccgaggg cttcttcccg tgaccatccg
    22801 aaaatagcag ccgcttggct gttccaatcg gtcaccacac caacggcatc gatcgtgacg
    22861 agcccgtcca gagctgtttc tacgatcgca cgtgtccgct cctggctctg cagcaacgcg
    22921 gcttcggctg cgcgcgccca ggaactctcc tgttgcgcct gttgatactc cgcccgcaat
    22981 ttcgccgtcg accagaagag gagcagcatc atgggaaccc acagcacggc gccggctaac
    23041 ccgaacttcc acatgatcga cttgatcggg tcgagaatgc gatcacgatc catccgtacc
    23101 aacacgtacc atccatgccc cgggaattcc ccgtacccct ttgtgtgggc gtagccggtg
    23161 acgacagatt ccccctgata ttcttcttcg acgaagcccg gttcgccgaa gtggctggcg
    23221 gtcacggacc gatgacccga ttgctgggta ttcaacagcc ccttgtgcat aacatcggag
    23281 tcgacaaaca ccgtgccctg tttcgtcacc atctgaatgg cgaccggacc ggtaaattcc
    23341 tggcggcctt cgagcgagcg cacggtcttg gtcgtcacct cttccaatac ggaggtctcg
    23401 acacgggtcg tgacgacgcc caggaaggtc ccctccgagt cgagaatcgg cgcggtgaat
    23461 gcgacggtct cgactccgcc gtcgccgtca tggacagcta tgtcatcggc ttgaatcttc
    23521 ctcgtttctc gcgcacgctg aaaccaatcg gctcggctgt agtctcgacc tcgtttggag
    23581 ggctcggtcg acgcgaccat taccccctgg ccgtcggtca cacccaacca gagatagatc
    23641 ggcgcgtaat tctctttcat ccactccaaa tagctggaca catagcgcgc gtcggacgac
    23701 cgtaaagcga aggccctggc catcatctgg gcatcgccga atcgctggaa cagcatgcga
    23761 tccagtttgt cggagacttc ggccgcggca atggtgagct cttccccggc cgcgacaatc
    23821 aaccgatgct ccacattgcg caggagaaaa gccccggcca ccaacgccac cagattcatc
    23881 aggatgatta actggggcaa ccagcgataa cgctgcgagt cgagaggccg ctggaaatct
    23941 tgcctgctca tgcgcgcata gcctcgctac agataaaacc ctggatctgc atcatcggat
    24001 ggtaggcctg cttcgacaga cgtaaccccg acagccccgc atcatccatc gtattgatga
    24061 actcggcccc ttcagacaac gcgcgcctgc acgtatcgcg aaacaggtat tgtgccaatc
    24121 cagaaatctc tcgatcagcc acttccagca agacacacca ggtatccttg ctcaaccaat
    24181 agccgaacgt gtaggcacga atccgtccct tgagacgaag gaccattcca tgcagtgaca
    24241 actccgatcc atgcgcccaa acaacttcgt gagcggacaa gacatcttcg agcaaatggg
    24301 tggcgaatgt gtccagcccg ccggtgcgct tttgctgccc ccactcgtga aacagcgcgc
    24361 ggcattccgc tcgatctcgc gggtgatagg gttcactagt gacgtctccg atccgttcga
    24421 aacgattgca gagcgcccgc tgcgatttga atcgatcacc ggccagagcg gccaggtcag
    24481 cggcccgata gaggtagtcc ggctctttcg gcgtcattcg atatcccacc gcctcaagct
    24541 cagcagccag cacagccgga atattttcaa ttcgactgac ggcggtcatg ccgttccgtc
    24601 gccgcatcag ctggaaccct tcctcgatag cattcaggat cgagccttct ttcaacggcg
    24661 gtaggggcat gaaccagccg tccggtgatt ggataaaaag gcaaaacgcc ccgtggagtt
    24721 cagtccacca ataggcgagc agcccattcc atacgtaatg gtaggacgct gaatgggccg
    24781 caagcggccg taccccagca aggccggatc gattcagggc atttgtgagg cgagggagat
    24841 cctccagcaa gagccggtga acggggtgag ctccccatcg ctccatcaat gcctgcgcga
    24901 ggtgggtgag cggacccagt acgaccacgt cctcctgaaa ccttcccacc agcccgggat
    24961 gattcacgat catgcggaca atttccggct gccgaaggag ttccatgacc cgttccgcgt
    25021 gcaagcgaat ggattccggg agttgctccg gcatgaaggg acacttggca tcccacccga
    25081 gcaacacttc gtcctgaact gcgttccaca tgatcgccaa cggatagagc tgacaatcga
    25141 gtggacgtcg ctcgtagatt cggcaggttc cggtctggct cgcgaacgca gggcaatgaa
    25201 agccctctcc ctccggatcc ggcacaacga gaatttgaca tccgccacgg tcagaaaaaa
    25261 ctcgctcgtc cacaccccca cggatggctt gatccacttc ttctttcgta aagtaggggc
    25321 gaagcgggct atcggggtcc gggaaccggc aacagatatc gcatcggaga caggtactac
    25381 tgggcacgaa ttgagggagg tcaggccggg tggctgccgc aggcgacgcc gttttcgtgg
    25441 ttagcgagtt catgcaggat tccaatagaa tggccatcat tctagcatta ttccccaggg
    25501 gggcctacta ggatatggtt tccccagcat cctctctcat gtaaaaaccc agaaatcctt
    25561 gaccgaaatc cccgtcgctg aaatactggc tgcatcgtcg ctattcactg acgcttggaa
    25621 acgatggcga actctccgga cttggtgcag tacgatccgc aggtgttgct gaattcgcaa
    25681 ccagtgatca tcaccgtcat cgaccccctt acccataaag ttgtctttca gaaccagacc
    25741 tctcagaata aattcggcga catctccaac ctaacctgcc acgaaaagat cgccggctgt
    25801 gcaaccccct gtgccttctg taggatgccg gaatgttttg aatcaggaaa ccccagtacc
    25861 agtgaagttt ccatcccgaa tgaggaacac ctcctcgtgc aatgggcaca agtcaagacc
    25921 gcgtccggac agacgcatat cgtcgaaacc atcaccgaca tcacagcgat caagcgccaa
    25981 caggccgaag ccgagggact cgtccagaga ttgtccagca ccaaccggga tctgatccag
    26041 gctaatcaat tgctgcgtga tcaatcgatt cgggacagcc tcaccggact tttcaaccac
    26101 tctcatttcc aagaaaccct gacccagctc tgcgcgctct ccctgcggtc tatgcgtccg
    26161 ctctcgctgc tcttcatcga ccttgacaat ttcaaacaga tcaacgatgc ctacggacat
    26221 acgatgggag atcaagtgct ccgcgaactc ggctggctcc tggacagcca gcagggacca
    26281 ggccgtggca tcgcacgatc cagcgacttt tccgctcggt atggcggaga ggagtttgcg
    26341 ctcatccttc cggacacatc gatggaaggc gcgctcagcg cggccgaacg gcttcgtctt
    26401 cgcgtcacca ccttgacgat gctgcccgaa ttgacggccc tcaacagcaa gccctgttcg
    26461 ctcacgtgca gtatcggcgt cgcgtcgttt cccattcacg cctccacatc atccgacctc
    26521 ataatcgccg ccgacaccgc cgtctatacg gcaaaaaaat ccggcaaaaa ctgcgtgcgc
    26581 atggcgacgc ccgccaaccc ttccctgccc ctcttcgatc cacttgtacg atcctaaagc
    26641 ccgtgttacg attcgtgcgt catgcggcat cggcatgatc catgaaatga acatatttcc
    26701 tgtctgattt ggctacctcc gaccttctcc accgtggctc cagtatccgg gacgctctga
    26761 tcgaagctgg gttgtttgcg cctcgagttg gggcgaaccc tccctctcca tcggaaaagc
    26821 cgcccgtcga aactttttgg cggatcggca tgaccccttt ggcgatcacg ccggaccagc
    26881 tgacattttt ccaagcccta gggcctcagt tgctcctctt ctatggcgcc gtgaatcgcc
    26941 tctatgccga gagcgtccgt aggacacagc ctgcgtggat tgccgattac ctggaccaag
    27001 gaaaaccgga aggactgatc acctacagcc gcatgaaacg gttccgggaa catctgcccg
    27061 ccgtcatccg tccggatatt atcccgaccc aggacggcat ggtcattact gagctggact
    27121 cggtccccgg cggcattggg cttaccgcct gcctctctca cgcctactcc gacatttcat
    27181 ctcagcactc agcacttagc actcaccaca tgggtttgat cggtgggcgc gatgggatga
    27241 tcgacggatt cgctgccatg ttacaggcac agcgtggcca gcttaatggg gtaatcgcca
    27301 tcctcgtctc ggaagaggca aaggattatc gaccggagat gacttggatg gccgctcagc
    27361 tctcggctcg cggactggaa acctattgcg tcgagccacg tcagattcgc ttcaccgaag
    27421 aagagcttct gatcaccacg ccggaacggc agatccccgt aggattggtg taccgcttct
    27481 atgaattgtt cgaccttccg aacataccca aggccgagct catccagtac gcggcgaaga
    27541 aagggtgggt gtccgtcacg ccgccgtata agccggcact ggaagaaaaa tcagccttcg
    27601 ggctgctcca tcatccgatg ttacaaccgt attggcgcca ggaattgggt gaagaccttt
    27661 tcctgcatct caccacgatc atgccccgaa cctggatact tgatcccgct cctgtaccgc
    27721 cttcagccac gatccccggc ctctcgatcg gaggccgtgc cacaatgagt tggagcgatc
    27781 tcgctggcgc gacacaaaag gaacggcact atgtcatcaa gccatccggg ttctctgaat
    27841 tcgcctgggg cagccgagga gtttccgtag gccacgacct cccgcaaggt gaatgggccg
    27901 ccgcaatcga ttccgccctt aaggccttcc cccgtacacc ctatgtgctg caggagtttc
    27961 acaaagggcg gcagtatcgg cttgaatact ttgactctgc cactgaagaa ataaaagaaa
    28021 tggcgggacg cgccagactc tccccctatt atttcgtcag tggcgaaaag gcggaactgg
    28081 ctggtatcct ggctacgatc tgtcctgtgg acaagaaggt catccacggc atgaaagacg
    28141 cgattatggt cccgtgcgcg gttgcggcag aggcgcatca gacgaaggac ttgagtgctg
    28201 agtgagttcc ttggaaaaac gaatggaaat cgttctcttg cctttcacca ctcagcactg
    28261 agcacacaca atgtcgctga ctctctacca tgtcgattgg tgtccagaat gtgacctcgt
    28321 tcgccagaag ctggccgaac tcaatttgcc atatacggac gtcatcgttc cggacttccg
    28381 gccgctgcga aagcaggtct tcgaggtgtc cgggcaatat tacgttccgg tgttgcagga
    28441 tggcgacaag gtattgagcg agacacacga aattcttgcc catcttgaac gcaaggccga
    28501 atcagatcgc ctcgaccagg gctgaagagg gagtgatccg atggaggaat ggagacgcat
    28561 cctcgctgag agcatcgtga agccgaagga tctcgccgat cgtttcgggc ttgacgagaa
    28621 cgaggtcgaa gcgatcgtcg ggccgtatcc catgcgcatc acaccgaccg tgctgggtac
    28681 gatcagagaa aaaggcgacg cgatctggaa acaggtcgtg cccgacgtcg ccgaactggc
    28741 cgacatcgac gcagaagatg atccactcga agaagacctc atgagcccgg tcccgcatct
    28801 ggtccaccgc tacccggacc gggtgctcct catggtcacc aaccaatgcc cgatctattg
    28861 ccggttttgc acaaggaaac gattggtcgg gaaacccgga tttttaaaaa aaggcgaact
    28921 cgaccgagct atcacgtatc tgcgccagca cagcgaagtg cgggacgtga tcctttcggg
    28981 aggcgatcct ctgctgcttc cggatcattt gttggagcgc gtattgaaag ctcttcgaac
    29041 cattccgcac ctcgaactga tccgtatcgg ctctcgcgtg cccggcacac tgcccgaacg
    29101 tatcaccccc agactgtgcg agctcgtgaa aaagtaccac cccatttata tgaatctgca
    29161 cttcaaccac ccggacgagc tgactccggc ggtcaaagcg gcctgcggaa tgctggccga
    29221 tgccggtgtg cccctcggcg cacaaacggt gttgcttaaa ggcgtcaatg atgatccgga
    29281 gacgatgaaa cgcctggtcc atcagctgct gctcgcgcgg gtgaaacctt actatctcta
    29341 tcaggccgac ttgaccaaag gaaccaatca tttccgcacg agcgtcgaga ccggcttgaa
    29401 catcatcaag gcactgcagg gcaacacaag cggcatggcc gtgccgcatt tcgtcattga
    29461 tgccccaggg ggaggcggga aaatcccact ccttccggac cactacctgg tgcacttgga
    29521 cgatgagggc gccgtgctgc gcaactatga gaacaagacc tttcactacc cccagccgaa
    29581 agcagggaac agccgtgaac tgccgatggt cggagcacgg gcgtcctacg acgattcgaa
    29641 cgaggccttc gcatcctgcg gcgacagctt tcccgatcgc gacggctatt ccagctgggg
    29701 caacagctgt ggcggggaat cggacgacct gtgaccaaga acgcggccca aggtattctt
    29761 ccctaccagc agatccgacg gctcgtaacc ggcggcgcga tcaccgcctc cgtggggatc
    29821 gaagaccgcc agattcaacc ggcgagcctc gatcttcgct tagggaagaa agcatatcga
    29881 ttgattagca gctttctccc ggagctgtcg gccatctcca gccggctcga cgtcatggat
    29941 ttctaccagt cggatctcgt gatgtacgag atggatttga ccggcggagc cattctcgag
    30001 aaaggccacg tctacctggt tccattgttg gaatcgttga aactccccgg caccatccgc
    30061 gcacgcgcga atcccaaaag caccaccggc cggcttgacg tgttcacgcg catcgtcacg
    30121 gacttgactg ccggcttcga cgaaatccgc gccggctatc gcggcaatct gtttctcgaa
    30181 gtggtcccgc gttccttcgc cattagggtc aagaccggcc aatcattgaa tcagatccgg
    30241 ttcatacgcg gcgaagcggc ggcatcggat gcgacgttgc agagcctgca ccgacacgag
    30301 ccgcttttgt accacaacac cccgaatcga aagcccctgc gggcgaagga tttccgaacg
    30361 gatcgcggcc tcttcctgcg tatcgacctg aagggagaag aaggggaagg ctcaacggtc
    30421 atcggttacc gggcaaagaa aaacagccac gtcatcgacc tgtcgaagat cggccattac
    30481 gcggcggcgg acttctggga gcccctctac cggcatcgac aggacagtct gctgctggaa
    30541 ccggaagaat tttacattct cgcctccaag gaacgcattc gagtgccgcc agattacgcc
    30601 gctgagatgg tcgcctacga agccgcctgc ggagagctcc gcacccacta cgcgggattc
    30661 ttcgatccgg gtttcgggta cggcgccgat ggcgaaatca aaggcaccca ggtcgtgctg
    30721 gaggtacgtc cgcacgacgt gccgttcctg atccacgatg gacaaacctt cttcaaggtt
    30781 gtatatgaca ggatgctcga tgtgccggat caactctacg gtgtcggcct cggatcgtcc
    30841 tatcaacaac aagccctcac gctgagcaag catttcaagg tgtaacccat ggcaccaacc
    30901 gatcttcccc ccccgcacga cccggcgcag cgccctccag cccaaccgac cggatcggaa
    30961 gggcaggaca gcggaagcga ccatcagatg catgtcgtcg gactgatggt tggaacggcg
    31021 ttgatgttta tcggctttct caacgtgttt ctttcgatca gcgggggctt tgaaatcaat
    31081 gtcgtgccgt ttttgattta cttcggaggc ctggcggtat gggccaacgc ggccattgag
    31141 actcccgccc ttcggtacgg ggtcatgacc gcggccattg ccctggcact gggattcttt
    31201 cactacggcg aagtgctgtt ctggcataaa caagtcgtct tctggtccac tgtcgtcatc
    31261 gtcatgtact tcatgttcaa cgaaccgaag aagcctgatt aatccccgcc cgatgacgat
    31321 ctccgtcatc atcccgacgt tcgacgaagc aatcaccatc tcagagaccc tttcgcacac
    31381 ggctagcctt ggctttgacg aacttattgt cgtcgacggg ggaagtgcag accagacccg
    31441 taccattgtc gagacgttca tttcgggaat tcagcactca gcactcagta ccgttcgggt
    31501 actgtcagct ccgtgctgcc gcgccgctca actcaatgcc ggtgcccaag ccagccgtgg
    31561 tgacgtgcta ttgttcctgc atgccgatac gcacctgccg gagacggcca agcagcggat
    31621 tgcggcggcc ctggccgaca cacacatcgt cggcgggcga ttcgacgtgc ggttcgatcg
    31681 gccgtcggtc ctgggacgaa tcatcagtac gctgatgaat tatcggtccc gctggagcgg
    31741 catcttcacc ggagaccaag caatcttcgt ccgccgcgat atcttcgagc gactcggtgg
    31801 cttcgccgac atcccgctca tggaagacgt cgacttcagc gccagactca agcgcgcagg
    31861 gccgacagaa gcgttgcgcg aaactgtcgt cacatccttc cgccgctggg agcaacaggg
    31921 ccccctgagg accatcctct tgatgtggac cttgcggttc ctgtattgga tgggtgtcag
    31981 cccatcgcgt ttagttgcat ggtacagagc cgtccgatag atcgtaaggg gtgaggggtg
    32041 tcgagcttaa gaaatagaca gctgcatgcc tcaagaccgg aatcctcttc ttcgcctcac
    32101 gcctcacgcc tgacgcatca cgcggccgcc ttagtgatat tcgccaaagc accgatcccc
    32161 ggccaggtca aaacgcgtct ctgcccgccg ctcaccgccg atgaagccgc aacgctccac
    32221 ggcagcttcg tgctcgacac gctggagcgg accaaggctg ccgcggggaa gctgaagctt
    32281 ccgttcgatc ggtatctcgc ctgttcgcca tcctcaacgg ccgtcttctt caagatcatg
    32341 gaggaacgtc aaggggtgaa gttgatcgat caggtcggcg aagacctcgg ggcgaggatg
    32401 aaacacgctt tcgagacact gttcgcccgc gggtaccggc acgtgctgat cgtggggacc
    32461 gatgttccct cactcccgct cgcacattac caacaggcct tggcgttcct ggacgcacat
    32521 gatcttgtcc tgggccccgc cctcgacggc ggctactatt tgatcggcct caacaagccg
    32581 acggcggcac tcttcgagaa tgtctcgtgg tccacagatc gtgtgctctc gatgacccgc
    32641 gaaagagccg acggcttagg cgtgaggaca gccctgctcc ctgcatggcg cgatatcgac
    32701 accatcgacg atctcaatgc attgatcgag gccggtgcgc tcgatgccaa gaagcccaag
    32761 aatgaacaat ctttttcaag ccggaccgcc ggcgcgctcc aactgatcgc taaacgacta
    32821 cggtccaggg caacataagg agtgatcatg agttctcatg tcgctgtcat caccggagga
    32881 gccaaaggaa tcgggcgcgg aatcgcgctc gaccttgctg cacaacaatg gaacgtcgcc
    32941 ttctgctatc ggaccagcga agccgaagcc aagaagaccg ccgcagccat caccgagaga
    33001 ggcgggcaac ccttggcgat acgctgtgac gtgtcggatc ccgtggccgc gaagaacttc
    33061 atcgcgcggg ttgaaaagga atgggggcag atcgacgcct tgatcaacgg tgccggcccc
    33121 tatcatcgtg taaacttgtt cgaagaaacg gtcaccggct ggaacgagat gttcgaggga
    33181 aacctccacc ccatcttcta tttagctcag gctgttgctc cgggcatgaa agcccgcaaa
    33241 tctggtcgga tcgtgaattt cagcatggcc aatgccgatc agatgatctc ccagccggat
    33301 gtcaccgccc actatattgc caaagccggg gtgctgattc tgacccgcac gctcgccaag
    33361 ctgctcgcgc cgcacggcat cactgtcaat gccgtctcgc ccggtttcat cgattccggc
    33421 agtgcgccgc ccgaggaact ggccggcatg accaagcgga ttcccgccgg ctacatcggg
    33481 accgttgaag ataccgtcgc ggctgtccga tatttactga gcgatcaagc tcgctacgtg
    33541 aacggcgcga acatccaaat cagcggtgcg tgggggatat gaggaggctg ggtggtctta
    33601 tctttgtgct cgcgcaacgc gcggcctcag aaggccctcg ttggatgcgc gcagttgagc
    33661 caagaccacc cagcctccta ggaaagacga agaaaggagc gtgagggaga catcatgaaa
    33721 acagagcagg aacggcaggc ccatcatcga tttgtgcagg cgctccagca tgagcatttg
    33781 acctgtgcca aacccggttg cggcggacca atggatgtcg tcgatctcac cccgcacaat
    33841 gcccgcataa aaacctatga agccgagtgc gaacgttgtc gcatgaagga acacattacg
    33901 ggaacggaac agaaccagcc gccgtgggat gacgcttcca tcaccatgat ggccgaggtg
    33961 catctactcc acgatcaacc gacctgcccc tacgacgata ctcccattac ttttatttcc
    34021 atgccgaatc cccgccgcaa agcccgttat cggctggcct gcttctactg cggcaggcac
    34081 acagagatga actggccgcc acccgaagcg aaacgatgac gggcaacgtc gttacccata
    34141 gccgtgcagc atgtagactc tctcttgttc ttttcagcct ctttgtctgt accaactcag
    34201 ctggggcagc cctacccaac aagccagctc agccggggaa actccttctc gaagcacgag
    34261 cagtcgcgtc ctccatccaa gaaccagacg aacgatcacg ggcgttgaac ggcattgtac
    34321 tcgagcagat tgcggtcgac ccggcaggag ccgtggaaac actcaagttg tttcccactt
    34381 tcccgaacag ggtccaccat tttgcccatc tggcttccgt ctatgcaaaa gccggtcggg
    34441 tcgatgaggt ggaaagaatg tatgccgaaa ttttagtcga agaccgctct tcacgggaag
    34501 ggaaacttgc tgccgccaat gcccttgggc aacttgccat tgcgcatgca aacacaggaa
    34561 acatggaaga agcgttccgc acactattcc gaatcaaaga gcgatccaag gaggagtctc
    34621 tcgcgattgt cgacatagcc acggcaggca tcgctgacgc ccaagccaga cacggcgata
    34681 tcccaggagc tgtccaaacc gcgttgagca tagttcgaga aaatccctac ccgctcatga
    34741 agatcgttgg cagtcgggct ctgaaaggaa gcgctggggg ggaggtcgaa gaaatcgttt
    34801 caagtctcga tgaaggggct caacaatatg cccagtgggg catcgtccag gcccggatcc
    34861 agcaaggtcg attcacagat gcccaggtcg cggcttcggc cattaagcct ggtcatgcga
    34921 aggctggcgc ccttttggaa ctcgcgaccc gccacattca gctgcggtcc aagcctcttg
    34981 cggtggtcct cttacaggaa gcggcgacat cggcccgctc gacaatcaac gaatggacca
    35041 gggccgatat cctgtggcat atcgccgccg gtatggcgga ggccggcgat gcgcccgccg
    35101 ccatcgacac agccaagtcc atcgagaagg atgggcatcg catctccgcg cttcatgata
    35161 ttgtaagaac gcaggccaaa cagggagaca tagcaggcgc tttcaataca gcactccagc
    35221 tgaagccagc atttaatata gaattcccag gatcgagcac ttacgaagtt gccgtttcag
    35281 atgtcttggt ccagctggtt aaatccggca aagcgcaaga ggcgcgagaa acagtcgagc
    35341 gtcttcagga catgcaatca gagcaccccc tgctctacag cgcgatcacg acagcacaag
    35401 ccgatacggg aaacatcaaa gccgcgaaga ccacgcttgc actcgttgaa acagagcggc
    35461 agcggaaggc aagaaagcaa gagctcttgg acctcgcaga gaagtttcgg gagggtcaga
    35521 atccagatga tgactctcga tggagaaagg tacagagcct tgacgttgcc atccgtcagg
    35581 cacaggaagc catcgccaaa gcgtacgcga ggaaaggcga tctcgccagt gcgatcgcaa
    35641 cggccaatga actgaatcta tcgtcagaac ggcttcggtt gatcaaagag attagcgcac
    35701 ttcagaccca gaccgccgcc accgagtcag cgctcagatg ggctcgcggt ctgccttctc
    35761 cttccgacaa agcctatgcg ctcttaggca tcgcgaccgg gctttccgaa gttaaaaata
    35821 agaagactcc gaagtagcgg gggtgtgttg tgctctcact gcacgcgtcc gaggaaggcc
    35881 gaaggcctgt tttccgttct caccagccca ccctcgcggc gccaagatgc cgcgttttcc
    35941 aggccggccg tgcgttgcgc gagcacaaga gcatgacagc cgctctcctc tccatcccct
    36001 agccgttgcg cgatcccccg tgagaggcag acgagcggat acggacgccg ccatcgccga
    36061 agagggcggg acacgacttg tcctcttccc gtcatcaaga aagagagtcg gcgggatggg
    36121 cgggtagcga ctttgcaggc gagccacgct gatgtgcctg cgcggcaacc gagcgaggtc
    36181 cgacagcacc ttccgtttca tgggaaggtg ctgatcgtaa accttcacat tctgatttcg
    36241 gggatgccga ggaaccgcag ccggttgacg ggcagctaca gcagctcaac gagcccgcaa
    36301 tgaagcgaat cggccagacg ccgtctctat ttcccaaacg ccttcttcaa cagcggctcc
    36361 atttcgccct tagccgccat cgggtctaac acatccgtat cgccatagaa cgtcccgtcg
    36421 ataaacactt ttggcagcgt gggccagttc gtcatcttcg tgagagcttc tctcttcgcc
    36481 ggctgcgaga gcacatcgat gacttcatac gggtacccgt acttgtcgaa gaattgcatc
    36541 gtttcccggg tgaacccgca catcggcatg gttttcgtac ccttcccata gatgagaatc
    36601 ttgtgggctt tgacttcatt ctgaatttcc tcttccattg tttctgccat cgtcggcctc
    36661 cttcttaaaa tgtcgcgata ccaaaccggg atgcgtctta taattcgtcc ttggttttcg
    36721 cggtcaactc gagggcatgg atacgtccgt ctttcatcgg tgcatcaagc gcctgataaa
    36781 tcaggcgatg tcgatcaagc aaatttttac cgtgaaaggt atcggacacg acgatgacct
    36841 tcaaatgatc catcgtaccg gtccggtcag tcatggtaac catggcatcc ggcatcgcgc
    36901 gccggatgta gtccgttaac gtttcttgag atatcactgt gcctcctatt tttatgcagg
    36961 ataccgtagc ggctttctga aaggcaattc gggcaagctc gcccaataac cacgtgacgc
    37021 aataaataag gggtcgcaat gtgatccagc gctgaatcac atctgattcg atatacttca
    37081 acttctcatc gctctttgca tccttccttg gcagctgcta ccactccaac gcaccaacaa
    37141 caaaagggat ttcaccggat aacgctcatg ctttcccctt ggtacagatc ttgggaatga
    37201 agatcggcaa gattgatcgt ttacggagga ggcgacttat gacgagcatg atgaccttga
    37261 ccgcgatcac agtgttactg agttcactac tgctcctgca ccgcatctgg tgacgagggt
    37321 cggcacacca tgatgatgcg aatacagaaa caacctggtg gtggatatct accaatggtg
    37381 gtgcatacaa accagctgcc agttgcttgt agtggagact cgttaaaata tactgataaa
    37441 taaggacatt ttcgcatcta ttctcatggc acatcagttg ctgtgtactc agacaggcaa
    37501 gagagatgag agtcctgaag caaaccgtcg gacagagcga agaggagcgg aacgattaac
    37561 ttactagtca cattaaagga gggtgctatg aacgaattta catcggggtt ttttatgggg
    37621 gacaaggaga caaatgggcg tgtgctggta gtggatgatg aagcggatat ccggaaggtc
    37681 gtccgcatga ctctgcagaa ggccggctat gacgtgctgg aagcggaaaa cggcgaaaag
    37741 gcgatcgaga ctatcaatgc cggtgagaac aggctgctcc tcgacgtcgt catctgtgat
    37801 atccgcatgc cgaagattaa cggcgtcgaa gcgatcgcgt acttccgcag caattatccg
    37861 cgcgtgccgg tgatcgtcct caccggtttc cctgacacag aaatggccac gtccatgctc
    37921 cgccaaggcg tcgttgacta cctcgtcaaa ccggtggaag gcgagaaact gcgagaggcc
    37981 gtgagccggg ccatggagca gagagaatta gcgcacctct aaatggctct cgtgcatgac
    38041 acaatgggga ggaccagcca gctggcctcc cctcctccgg aggtgaacgc ggtgcaacac
    38101 ctgacggaca gagttgtcgt gtcaaaaatc gcgccgacca aagtggcgat ccttggggca
    38161 ggacgaggag gcagcgctct tcttgacctc ctccatcaga ttccttcgat cgagatcgta
    38221 ggcattactg atcgcgacct gcgcgctcct gggatccaac gcgcgaaaga cctcggtatt
    38281 cccgttgtcg ctcaggcgga ggaactcgtc agccatgacg ggatcaacct gatcatggat
    38341 gtcactggcg acccttcgat ggaacggttt gttcacgcga ataaacggcc tgaagccgaa
    38401 gtgctgagtg gaccggcctc gcgtgtattg tgggaattgg cccaacatga agccaagctt
    38461 caagccgagc tgtttcatgc cgagaaatta gccggcatcg gctcctttgc caccggaatc
    38521 gcacatgaca tcaataaccc gctacaattg atcatgggac tggcggagaa tcttgcagac
    38581 gagaaagact tggacacgat ccacgaacag gctcgcgaga ttatcggtgc cgtgcgaagg
    38641 accagcgcca tctgccggga tctcacgcaa tatgccagga gaaagtcgga cagcggtgag
    38701 acgatggtgt atctcaacgg acgtctggac gaagcgctca agatcgcccg ctatgcggtg
    38761 agttttcacg atatcacggt gcttaaggaa taccagcaag gagccgtggc caaggggaat
    38821 gcagatgagt tgctgcatgc gttcgtgaac ctgattacga acgcagtcca cgcaatggat
    38881 catgggggtg gcatgctcac gctcgccacg gcaatatccg acggcggggt gacggtccaa
    38941 ataaccgata ccggatgcgg catccctaag gaaatgttgg gacagattta cgagccgttc
    39001 tttaccacga aggagccggg gaaaggcacg gggctcggcc tctacaacgt aaagaccatc
    39061 gtcaccaaga tgcatggaaa gatcgcggtc aaaagccgcg tcgatcacgg cacgacattt
    39121 acgatcacct ttcctcccgt cccagaggct ccgtcgtgac tcagccgccc ggtaaatcct
    39181 catcttcgac ccgcgggtgg agactccaga ccaaactgat cgtctcgatg ctgctggtcg
    39241 gcgtcgtccc tctgctcgtc ggcctcggca tggcgttctg gcaggggtcc aaagaaatcc
    39301 aagaggtcag cggagaaagc tttaaggcgt tggcgactga agcggcgaga aaaatcgaca
    39361 ttctcttggc cgaagaaatc gcccgcagct cccggatcac cacggacccg ttcatcgtgc
    39421 gcgagctgga acggaggcgc gatgcgctgc aagaccccgc gacctcggcc tcctacgacg
    39481 acctcggacg caaatgggat tcccaagacc ctgccacatt gaaagccctc accgaaaatc
    39541 ccatcgcgaa cctcctgcgt gagtattact cgggagcgcg cagtgaaccg gaccaattga
    39601 tcccgcaagt cgtccgcggc gcaaccaaga tgttcttcgt cacggacatt cgtggagacc
    39661 tcgccggtgc cctcaccacc aagccgccct tctctcatgg gaagacggcg tggtggcaag
    39721 gcgccttcaa taagggcatc gggcagctct atatcgagga tgtacactac gacgaccggg
    39781 ccggcactta cgcatttacg atctcgcttc cgatcatgga cagcctccgg tacgaggcgg
    39841 tcggagtctt gcaccgcgtc atcgacgcca aggagttttt ctccccctcc acccaaccga
    39901 ttcgattcgg caagaccgga cacgtcatgc tgatcgacag ccggggcgtg gtcatgagct
    39961 gcccgattct tcccaccggg gtcagcctct cggatagcag cttgatccct ctggtgaccc
    40021 ccatgcaccc gggctggaca agcgcgccga gcgacggcca tggcggtcgc tcctcctcca
    40081 tcatcggatt cgcgccgctt cccgaaacga gccgcgcgac caacggcgct tacgagagag
    40141 gctcctggca tacgttcgtc tggcaatcat ccgacgaatt gttctctcct atcagacacc
    40201 tgttcacatg gatgaccgtg ttcggattca ttgccgtcgg cttgcttgcc acgctgggct
    40261 acctggcggc aatccgtatc gtgacgccgg ttcgtcaact gcaacaggcc gcccaatcaa
    40321 tcggccgtgg ggaattgcgg caatccatca acatcaagac cggcgacgaa ctagaagcgc
    40381 tcgcggaaga atttaaccgc atgaacaaac aactggaggc ggccttcgct ggtctaacgg
    40441 atcaagtcac gttgaaaacg caggaagtgc agtacttaca acgatccaca gaccaaattc
    40501 tcgatgcggt gccgacgccc atcttgatcg tcgacgacca ggaaaccgtg cagtacgtca
    40561 accggacagg acgtcaggcc ttcgggctgg ctgaggattc cgcctccacc ggacggctct
    40621 ttgatatcct gcctgtcgaa tcctcacagc aggagcggct acggcaggag ctctggaagc
    40681 atcagatggc aggaacggcc tccctgccaa agattgaaga gtcggctgct gtcgatcatg
    40741 tcagagatcc gctcgccccg ggcacaggcg cgactcaacg cgagcggcaa ggcgaattgc
    40801 gattggacgc gcacgtttat cagtatcagt ggttccgggt catcgggcgg ccaggagaat
    40861 cagaccgcat cggccttgtc ctccgcgaca gtaccgacga tagccgtatg caggagaagc
    40921 tcattcaggc ggaaaagtca ggaagcctgg gagtgctcac cgccgggatc ggtcatgaac
    40981 tgaacaaccc gttgttcggc attttaggcc tgggagaagc gctgcaggaa gaatgcgacc
    41041 cggccagaat ccgatcatac gcacaggaca tcgtacagca aggcaaacgg atggcttcga
    41101 tcatcagaga tttcaccggc gtcgccgcac gcgaaacgtc ggatcagcga cagtccgttc
    41161 atcttgaacg ggaactcgat caggccctcg ccacgatcac ggcgagcctg ggcactcccg
    41221 aactgaccgt cgcgaaacag tacgccggag acactacggt gtcggcactt cccgaccaac
    41281 tcagacaggc attcaccaat ctcttgatga acgcagtcca ggccatgaaa ggaagggggc
    41341 tgttgcgcct ctcgacggtt gtggccggga catccgtcgt gacgacgatt gcggatacag
    41401 gccccggcat tccgaaagaa catctctcga aaatcttcga cccgttcttc acgaccaaag
    41461 gccagggcga aggatccggt ttgggattga ccgtcgcgcg ccgcatcatc agaaagttcg
    41521 gtggcgatat tcagttggag agtgtcgaag ggaccggcac atcctgcatg gtgacgcttc
    41581 cgattctttc ctctacgccc tcgtcaaagg aggtgccatg gaccgcttcg gaatctcgcg
    41641 tgctgccgtc gcaaccatcg cgctcctgac cgttggactg tggtggggga ccgtgggctc
    41701 ttccgccaag gaagaactca agacagctgg aatcccgccg gagaaggtcg caggcttcgt
    41761 gcatgccgtg cttcaggcgc accggaccat ctatacgacc cagatcgtca atcgcttgca
    41821 ggaaaaagga atcgtggcgg cggcggaaca ttgggaacag gagaatgcgc tcccgctccc
    41881 ggctcagttt ctacaacatt caggacggct ggtcgctgaa agcggtcagg gaatccgcta
    41941 tcggctcatc ggactgtccc cgatctatca acggaatgcg ccgtcgacag acttcgagcg
    42001 gaaggcactg gaatcgttga atcgctcgcc ggatgcgccg gtgacgggca tcgtcaccag
    42061 cggacgcaaa caatacttcc aggcgatcta tccagaccgc gccgtgtcga gcgcctgcgt
    42121 caactgtcac aacagccatc atctcagccc gaaacgagat ttcaaattga acgacgtgat
    42181 gggcggaatc gcgattacga ttccgatcga gtgaccaaga cggcgggaag gcgtggagac
    42241 actctcatcg atgggcgaaa tgaaggttcc ggcgctcgcc atcgacaccg aatcacacga
    42301 gggggaatta cccgtttgat tccttgtcga gaacgacccc ttgcttggcg cggtgtgaag
    42361 agaggtcgcg aaccgctccg ggcaacttgg gtaactcatc cggccaggcc gtgacaccca
    42421 tatctgtcac cccctgaatc ttcgcacctt cttccatcat cagcaccggc gtatggacct
    42481 cgccgatcag gattgccgtc ttcagcagct gaacccgctc gaccgctgtc accgttgcct
    42541 taattcggcc gctgctgacc acggttcccg cagcaatggt tccctggacc agaccatctt
    42601 cgccgacgat gacttggcct ttcgtgtgga tgtcgccatc gaggcggccg tcaatccgca
    42661 cggtcccctc gacacgaatt tctcccttga gctgcacccc cttggccaag agagtgatgt
    42721 tgtcgtcgcc tccgaatcca tttttcttca tgcgtggctc cttccagctc gaatttcact
    42781 gtcagcttac atcaatccca ccgggagcac ctagcagatt gttgaattac ggttcggacc
    42841 ggcgaaagtg aatgagaccc ttcgcaatcc aatcacgaga agccgaacgt ctgccgcagt
    42901 cgctcccaga tgccgccgcc atggatttca caatacacgt ccggttcggt accctcgatg
    42961 aacacttcgt cgacttgttc gggacattga gaggtcgcga gctgtcctgt cttgggatcg
    43021 atcgcccgca caagtacacc cgacggtcgg gggaacggcg gcgcatgatc cggcaacatc
    43081 cgcctggcga tttccatcca gatcggcaac gccgcttgag cgcctgtcaa ccgtaccgcc
    43141 cgttcatcgt cgaatcctac ccagacaccg atgacgacat ccgaagtgta gccgacgaac
    43201 caggcatcgc gatacccgtc ggtcgttccg gttttgccgg caatcgcacc attgagaccc
    43261 aacactttgg cctttgtcgc ggtcccgcgc tgtatgacgc cctccaggag ggaggtgaca
    43321 agataagccg cctgaggcga cacggcctga cgccgatcgg gagccgcact ccacaaggcc
    43381 gtgccttctc ggtcggaagt cgcgcgcaag ctcgtcggcc gaacccagat gcctccatta
    43441 gccacagtcc cgtaggctgc caccatctcc aacaacgaca cggcagggct tcctaacgcc
    43501 atggaaagat catcggtcaa cgccgtcgtc acccccagct gacggatcat ctcgagaatc
    43561 ggctgtgtcc ccaccgcttt cgccacccgc accgccggca cgttcagcga ttgttccagc
    43621 gcgctacgta tcgtcacgtt cccacgaaat tgacggtcat aattttgagg agaccacgtc
    43681 ccgttttccg attcgaacgt gaccggttcg tcggcgatca gacttgcagg cgtcaggtac
    43741 ggctgttttc ccattcgact cgcttcaaaa gccgcgaggt agacgaacgg cttgaacagc
    43801 gagccggcct ggcgacgcgc ctgcaccgct cgattgaact ggctgacgcg gtagtcccgt
    43861 cctccgacca gcccgaggat cgcccccgtc gatggttcga gcaccaccac ggctccctgc
    43921 agcgactgct cagagctttt gagcgccgga taggctcgct cgagccttgc cagcccattc
    43981 tccaaaccct cggccgcaac ctgctgcacc agtggatcga gggtactatc gatgcgcagt
    44041 ccctccggta atggcgtccc gatggcgtct tcgacctgcc tcaagaccgt atccacaaaa
    44101 tatggtgcat cggccaagac atcgtcgggg ggcgcaacgc gtaccggctg gttgacagca
    44161 atcttccatt cctcttcgtt taggtgcccc tgatcccgca gaagccgtaa caccacgtcg
    44221 cgacgctgct gggctagctc gagatgcttc gtgggcgcat aggtgttcgg acccttgatc
    44281 aggcccgcaa tcatcgcgat ctctccggct gtcagttcgt ccagcgactt cccgaaatac
    44341 cgatgagccg cctccccgac tccatagatc gaaacgaatc ctgcctgccc caggtagatt
    44401 tcgttcagat agctttcaag gatttcctgc ttccggtatt tggtctccag caccacggca
    44461 gcgagcgctt ccttcacctt tcgaacaaac gttcgctgcg gcgaatagaa aagattcttc
    44521 gccagttgtt gggtgatcgt gctgcctccc tgcaccaccc cgcctttggt caggttcctc
    44581 cagaccgcgc gcccgatggc gaacggatcg atcccaatgt gcgaatagaa tcgacggtcc
    44641 tcaaccgcca acaccgtgtc aaccaggcgc ggaggaatct gagatagcgg aatccattcc
    44701 cgcacttgac gcgaagtacc ccgcaatccg ctgatgagct ccggctcgag aaagacggga
    44761 aagatcgggc tccggtccgg ctccgacaac acctgggtga catggccttg ttccaactcc
    44821 agggaaacga tgctggcctt tgtcagattc tccggctggg catgcaaata aatcgtgagg
    44881 ctctgcgggg tgagctgata gtcgcccggc gccgccaccg acccgctgac cgccctgtaa
    44941 ccaagccgct gcaatcgctc ctgcaatcgc gcggcgctca cggaaaggtc cgacttgagc
    45001 tggaacgggg cggtatagag gcgcaatggc ggatgatctt cgctcttggg taattcaaga
    45061 aacgtcgcaa ggtaggcccc gtagagcgca agcccggcga tcaggagcac gaccattcca
    45121 gcgacaatca gccccccgcg aaagaaccag ggtgagcgca ggatatgact cagccgcgtg
    45181 agcatcttgt tcagcatacg tgagcgcgag tgggaaagcg attgcgccgc accgggacga
    45241 ggcgtcgagg ctcgcggagg gttacgcccg gaccttctgg tcgggacgca gcagcgaacc
    45301 cacaaccatg ccgactgtag ccatcagcaa tcccactaac tgagccggcc agaccggatt
    45361 gtcggaacca aagaattcca ggcccaacca gacaatcaac cctgtgacaa tggcacagag
    45421 cgctccccag gaggtggctt ttttccaaaa cagccctgcg cataacggaa cgaaggcagc
    45481 caccaaggtc accttgtagg tgttcacgac cagcttgtag atggttgcgt ccgaccagag
    45541 ggcgatcgcc agcaccacgg cggcaaaaca gatgagcacg atccgcatgg tccgtaacag
    45601 agcccactca tccatgtgga gcatgattcc tttgatcacg ttctcgctca ggatgaccga
    45661 tggagccagg agcgtggcgc ttgaacagct cataacggcg gagagcacag ccccgaaaaa
    45721 catcacctgc gcgaacgctg acgtatgttc cacgatcaag gtcggcaaga ccagttggga
    45781 atccctttgc aataacgccc cgaacttctc gggatcgacc agcaacgccg aatacgcgat
    45841 aaacatcggc acgaaacaaa aggaaaagta gagcgccgcg cccatcaacg atgcccgaac
    45901 cgccgtgcgt tcgttcttcg cggaggtgat gcgctggaag acgtcctgct gtggaatcga
    45961 gccgagcatc attgtaaccc acgcgccgat gaaaggaatc cacgcgttca gtgtggccgg
    46021 aggaaagaac tccaatttcc cggcctgcgc ggcatgggca atcactgtac cgacgccgcc
    46081 ggtcaggtcg ctcatgacgc tcacgatgta gagcagcccg cctataatga cggaaatctg
    46141 cacaaagtcg agaatcgcaa cggagaacat cccgccgaag gtcgtatagg tcaagacgat
    46201 caatgcgccg atgaccatgc caagcttctg actcactgct ccgtccgtga cgacgttcaa
    46261 caccaatccg agcaccttga actgcgccgc aacccatcca agataggaag cgacgataca
    46321 gagcgtacag agcacttcca cacgacggtc gtaccggagc cgatagtagt cgccgacagt
    46381 cagcagattg agccgataga agcgaggcgc aaagaagagt cccgcaatga tgaggcaaag
    46441 gctggagccg aatggatcgg cgaccacccc gcggagaccc tctttggcaa acgtcgccga
    46501 gattcccatc accgcttcag cgccaaacca cgtcgcaaat accgtggcca tgacgacggg
    46561 aagcgggaga ctccgtcctg caaccgcaaa atccttcgca ttacgcacac gggttgcagc
    46621 gtagagtccg atgctgacgg agacgaagag atagagaatg acaaagatga ggatcatgca
    46681 ggaatcgaac acgaaccagg ttgggggtgc agtgtacctt cgcacgcatg aaggcgcaac
    46741 atcggagagg gccggggccg gctttgaatc cggcaccctg caatgacaat cgacaattag
    46801 ttcttgccgt cgttcttgtc cagatcgtct ttattgccgt ctttctcttt gtcacggccg
    46861 tgcttgcgag ctttcttttc ggctttgcgc ttctctttct cggcctttcg ctcggctttg
    46921 cgacgctctt tttctgcttt ccgctccgcc ttgtcgcggt ggcgctcccc acccattttc
    46981 ttctctccct cctgcttcat ctccccggaa gaaggagcgg gcataggagc cggcgcagga
    47041 gctggggccg gaatggaagt cggtggtgct gtcggcgcag gagctggctc ctgggcgaat
    47101 gtggttagcc caaatgccaa ggcgatgacc gctgttgaaa gctgcacagt cgcacgattc
    47161 atcgttgagt cctcctttga tgacaagaaa tgagggatgc tctctccctc tcgttcgcgt
    47221 acccggcata ctttctcgca agaaagaagt gccttgtcaa ctccggagca gcctcgtatg
    47281 caattgatcg cagacgtctc tccatacctg tggtactccg tatgaaagag agggccggag
    47341 gctctcgtcc agcgactcgg ggcatcccgt tcctccagcg gtcagactcc taaaaaagtg
    47401 ttgagcttct ccgccgggtg tcatattgtg ggcccagatc tttttgaaat ttgtcacgaa
    47461 aggacaggtc atgcggactc tcattgcaat cgtactcgga ttgttcttca tcggaatggg
    47521 aacgtcagcc acggcggctg accccaccac cgatgaccaa aaaactctct acgcgctggg
    47581 cttggccatc agccaatcgc ttggtgcctt taccctcaac gagtctgaac tcgagatggt
    47641 caagaccggc atcactgacg gcgtcttgaa gaagccgcag aaggtcgacc ttcaagcatt
    47701 cggtcccaag atccagcaac tccaacaggc acgcatagga gcgcttgccg agggtgaaaa
    47761 gaaagcgggg acggcctaca tggcaaaagc ggcagcggaa gcaggcacca agaaaaccga
    47821 gtccggaatc ctcattacga cgctcaagga aggaaaaggc ccggtaccga aagccaccga
    47881 cacggtcaag gtccactacc atgggaccct ggttgatgga acggtcttcg acagttccgt
    47941 gaagcggggt gagccggcga ccttcgcgct gaaccaggtg atcaagtgtt ggacggaggg
    48001 ggtgcagcaa atgaaggtgg ggagcaagag caaaatggtc tgcccctcct cgcttgccta
    48061 tggtgaccga ggcgctccgc cgaatatcaa gcccggcgcc acgttgattt ttgaagtaga
    48121 gctgttggag atcgtgaaac agtagcagtt cgtgcatcgt cgggtcgggc tcgcgcgtaa
    48181 tcgcgacccg acccagcacc aaccgcaacc ccatgtacct acttgcagtt ctcctggcct
    48241 accccgtaca ctgacttcgt tgcgatccag cctgacccca tcagtcctgc ctatcgtcta
    48301 tcatcacact atcgactgat tacgtttacg ccgccatgcc acctacgatt ctcttgagtt
    48361 gcgagtccat cagcaaaagc tacggcgtga agccgctgtt tcgcgatctg tcgctcgcgc
    48421 tgtgtgaagg cgatcatgtc gggttggtcg gccccaacgg atcagggaaa tcgacgctgt
    48481 tgaagatcct tgccggcctt gaagatccgg atgacggcac cagatctgcc cgaagacagc
    48541 tccgcatcgg ctacgtccca caggaatcgg tcttcccgga cggccacacg gtcgaagagg
    48601 ttcttgccca ggtcctcatt gatgaaggct taaaccctca cgagcatgga gggcgcatcg
    48661 ccaaagcctt gagtctcggc gagttcactc gcgcagacga gtcaaccggc gttctgtcgg
    48721 gaggctggaa gaaacgcctg gcgatcgcac gctcgttgat gatggaaccg gacgtgctgt
    48781 tgatggatga gccgaccaac catctggatc tcgagggcat tctctggctg gaacgattgt
    48841 tacgcaacga gtctcaagcg tttctcgtgg tcagccacga ccgccgcttc ctcgagtcga
    48901 tcgcaacgag gatgctggag ctcaatcgct gttaccccca tggggtgttc gaagccaaag
    48961 gccgctacag cgatttcctg gagcagcgcg acgcggctct ggcagcccag gccgattatc
    49021 aggcgtcgct cgcgaaccgc gttcgccgag aagtcgaatg gctgcgccgc ggaccgaagg
    49081 cgcgtacgac caaagccaaa tcccgcatcg ataccgccgg gcaattgatc gatgaactgc
    49141 aggaaatgga atcgcggcag aagcaggcga ccgccgggat cgacttcagc tcatcgggac
    49201 gaaaatccaa acagctgatc gtcaccacag gactcagttt ctcgctcggc gggaaaccga
    49261 tcgtctccgg tctcgacctc cggctcggac cgggacagcg actcggcctg ctcgggccca
    49321 acggaagcgg aaaaacgact ttgctgaagc tgctcgccgg ccaactgaag cccgacagcg
    49381 gatcggtgat gcatgccgat aaactgcgta tcgtgacatt cgagcaacat cgcgagtccc
    49441 tcgaccagca ggccaccctc cgccgcgccc tcgcgccggc cggtgacgcc gtcgtctacc
    49501 aggatcgctc cgtccatatc gcttcctggg ccaagcgatt tctgttcaga ccagaacaat
    49561 tggacctgcc ggtctcacgg ttatccggag gcgagcaagc acgactgttg atcgccagac
    49621 tcatgctcca gccaaccgac gtcttgattc tcgacgaacc gacgaacgac ttggacatcc
    49681 cgacactcga tgtactggag gacagcctgc tggaattttc cggtgcgctg gtgcttgtca
    49741 cacatgaccg ctggctcttg gaccgtgttt cgacaacgct cctggcactt gacggacagg
    49801 gtcacgccga atggtttgcc gattatgcgc aatgggaggc ggcgcaaaca cgcaagactg
    49861 cggggaagcc atccgctgcc tcaaactccg gcgatgcggt ggattcacgc cccatgccga
    49921 aacggaaagg attgtcctat caagatcagc gcgagtggga caagattgaa gggaatattg
    49981 tgaaagccga agcggcgctc gctgcatgcc gagcggccgt caacgacccc tcgatcgtct
    50041 ccgatgccga cgcgctccgc acccgctatg ccgcgctcga aagcgcgcag gatgtcgtcg
    50101 aacaactcta tactcggtgg gccgaactcg aagagaagcg cgccaaggtg taaatcggtt
    50161 ccgtcctcgg agagtgtgtc accgaatcca tggaggcgca gtcgtcgggg gaggcgtgag
    50221 tcgctcaagt gttccgggcg gccgcttttg gggttctgcc gggagtgaaa agccttccgt
    50281 atcaagatcc cgcttgaacc gaaccacgag ggcgcccatc acatcccacc gctggaagtt
    50341 tttgcgcgca gcgcgaggat gctggttgtg acaatcgacg cacgattgca ctaaggccaa
    50401 gtccgccgac acagctttga actgatcccc gtccatgaag ctcacgaaac gccgttgtcg
    50461 gtccttctcc aactgtatca acgcatccgt ctcagcagcc gtcttgggac ggttcgcagg
    50521 attcaggggt atcaggccga tcagcccgat atcgaaactc tccacctgtt cggccgctgc
    50581 tttcacgaat tgtgcgggaa gcggaaggaa atgcgcgtcc ttttgccagt cttcttgcgg
    50641 cgaagttccg cctcccctcg tatgctcgac gacttggagc acataggccg tgcggaaggc
    50701 cttgacgact tcaaggatat atcgagccgt ctgttcaaag gacggttccg cggttgtcac
    50761 ggtccacccg agcatggtca ggaggctcgc gagactgaga gtcgtgacga cttgacgtgt
    50821 tcgcacgaag agtcctcgca attcaggagg cgtaatgatc tctcgagatc gcgggaagcc
    50881 tacctgatca gaacttgact cgtcaacgcg agatccacct ctctccgtcc tgcagtggtt
    50941 tgcgagacca caacgcatgt ttatcccctg gcatgatgcg gcgaagcgca acaaacaacc
    51001 acatcacgtg aggaaaagcg cgcgcgaggc caaccttgtg aggcgcaaga atccgtgtaa
    51061 aatagtctga tgcattctct cacaaggcct gcagcgtttc tcgccatctt caccctaacc
    51121 ctcacgtccg gatgcgagac aacggatcgg ttgctcaccg gcgcagagag agctgtgacg
    51181 agcaggaccg gacagacggt cattgacttg gctgcaggca aagatcccgc gcagatcgcc
    51241 aaacggcggc tcgaccagta cacgcgtgac cctgaagcgt tcctcagaga cctgcgcgcc
    51301 gcccaacgcg acttccaagc gttatacgat gcgctcagcg gcaaggtcgg cgagaagtgg
    51361 gggaagaaag agatcaagct gcctgaacag aagaaatacg tgaagtacac gcagaactac
    51421 cggagccgcg ccatcgtgga cttcgacaag ggggagatcc tcgtcgaaac ggtcgacaac
    51481 caggcccccc ggcgcagtct gaaaaacgcc gtcgtcacaa cgctgttgac acctcaagat
    51541 ccccgcgctg tcgacctgtt ctccgataag gaaatcgtgc tgacggggga caaggagccg
    51601 tacctcctag gactggtctt ggaccagcag ggcaagccga ttcgttcgcc gaatgaggcg
    51661 gagccgttcg cggaacattt gctcgacaac aaattcggta cgagggaagt cgaccaggac
    51721 gggacgaaaa agacggcggc ctttgtcacg ataaaaatgg cggccaacct cccgcagcga
    51781 caagccgaga aataccgccc gatggtccag caattcgcgc aacagtatcg catcagtccc
    51841 agtctcgtct tcgcgatcat ccgcacggag agtaacttca acccctatgc cgtgagttca
    51901 gctccagcct acgggttgat gcagctcgtt cccacgagcg gtggcagaga ggcgtaccga
    51961 cgagcgaaag ggaaagatat cgccccctcg cgcgactacc tcttcgatcc tgagaacaac
    52021 gtcgagctgg ggacggccta tctcaacgtc ttgaccttta cacagctgga tgaggtcgcc
    52081 aatcaggtgt cacgcgaata ctgcgtgatt tccgcctaca acaccggccc tcggaatgtg
    52141 ttgcgcgcct tcgcaaaaga aatcgaagcc gcgctgaata tgatcaacag ccttcaaccg
    52201 gctgccgtct acgaccaatt gcggcaccac ctgccctacc aagagaccag gacgtatttg
    52261 gaaaaagtga cgacgttcag aaaagctttt atcagtgcgg caaactcgtc aaattgaacg
    52321 gcgggctaac agggaatgtc ggttgccgga tcgcacagga gcggcgggat ctggagaggc
    52381 cgcatccacg tgctggaagg atcttgcccg ccaggcgtac cggaaaccgg cggacctccc
    52441 ggcatgcccg gaaggcctcg gccggccgac ccgaacgact tcccgaggct cgagcttgga
    52501 atggtaccga cacctggaac cggtgtggct accgggcccg ccggtgtcgc agaactgctg
    52561 acgccgctca cattcgctgc agggccaacc tgaggagcag gagccggttg gatctgcaga
    52621 ctgggaggag gcggcgacga cgtctgggcg aagcctgtga gggctcccga tagcacgcct
    52681 ccgcagaggc tgacggctag actccaccgg atgatcggac gaagcataac tcctcctctc
    52741 gcattcatga agcgactatg gttcgaacat gctcgggacg aaacccaagg ccgatcatcc
    52801 gagccgggat ccggcgtaag acgggaaggt gggccatgat cttgaatgcc aagggaggca
    52861 tcaccggtcc cgtactgccc agcgttgtct tgatgacacg attctggacc atcaactgaa
    52921 ctcgctgcgt catcttcgta ggccactcgc gccgaacctg caccctttga agatgctccg
    52981 ttgccagatt cctttcatag agtggtcccg caagaatatt ggcagcggcc acggcgtcct
    53041 gaatcgcgag attgattccg acgccaccga ccggtgacat agcatgcgcc gcgtcaccga
    53101 tgcagagcaa ccctgctcgg taccaccgac gcaatcggtc gacctgtacc gttaacagct
    53161 taatgggccc ccactcgtgc aattcgccga ctcgatcgcg cgcaaacgga gccagagctg
    53221 ccacattctc ccgaaacgcc ggcaatccct tcgcctccag ctgatctcgc gaccccttcg
    53281 caattacaaa gccgcattgc cagtactcgc cccgattgag cataatgaaa atacgtcccg
    53341 catcgaatcg tcccatcgga tccaccggat cgccgggctg tcgcgagagc cgaaaccaga
    53401 ggacatccat cggtgcgccg aattcatcga tcgacaatcc cgcccgagct cgaaccacgg
    53461 aatgccgtcc gtcggcacca acgaccagat cggcacggac gtctaatggt ccgtccacgg
    53521 tatcggcgcg tagcccgacg atcgtaccgg cctcctcgat gaggtccgtc acgtccgtat
    53581 tcatgcgcaa ctgaaaagtc ggataccgcc gcccttcttc agcaagaaag ttgagaaagt
    53641 cccattgggg catgaaggcc acaaacccgc atcgcgtcgg cagcgtcgaa aagtcggcca
    53701 cggtaatcgc caagtcgcca aaccgcgcat tgagacgcgg cactttctga tggggcaagg
    53761 ccaagaatcg atcgagtagt cccagctcat gcatcacctc aagcgtggag ggatgaagcg
    53821 tgtctccacg aaaatctcgg agaaagtccg cgtgcttttc caggaccagg accgacaccc
    53881 cggcgcgagc cagcaagaaa cccagcatca tgccggcagg accaccacca gcaatgcaac
    53941 aacggacaga agccgtcagc gtgcccatgc accccctcga acgaccgtga ctccagacga
    54001 ggctacgctt tactgtttaa cgaggcaagt gggcgctgga gacacgggga gcaggagact
    54061 cttgatgatg cgttctggag ctcagtccct tggaccaagg aacagagaag ggttacgaga
    54121 tcgtctcgct gacctgattc gagacaccgc cttcataccc cagtgtctca aacgagctaa
    54181 tcacgatata gccggccgtt cccttcacgg gaaccagccg accaagaatc gggtggatca
    54241 tgtctcggac ccacggcaag aatcgcaacc ggcatcgcgg atctcgtaat ccgcaactcc
    54301 tccccggcga tccagctgga tcaggcaaca ttcgtcgagc atctgcttga gttgttttcg
    54361 gccacgctgc acccgggact tcatccccga tagcgaaagc ccgaggcggc gggccgctgc
    54421 ctcttgtgtg agaccttgaa gttcaaccat catgaccgcc tcacgatagt ctttcgataa
    54481 tcgatccagc atcggttgaa gacaggctgc cagctccgtg cggagttggc cggattcgtc
    54541 atccgagggt aaggagaccg gaacggttgt ctccacgtca ccggccaagc cgacaggcaa
    54601 ttcacgtcga cggtcaggcg accggtaata atcgacgatc gcgttgcgcg taatctgata
    54661 gagccaagcc ataacgcgat cgggatcgtt caaacgatcc acacggcgat gcactcgcag
    54721 aaacacctcc tgcaggatat cgtcggcttc gacttcgttt cgcatacgtt tcgcaataaa
    54781 tcctcgtagg ctgtcgtgaa cctcgcgcca gatttctgtc aggtcttgtg ccattctaga
    54841 tcccgcctgc gtgatcccat acggtaccgg ctctttacga cgccacgcaa gcggccggtc
    54901 cagccgctcc cgcatgcttc ggcacgcagc aaccgctgct cgagagcggt ccatggacgg
    54961 gcaactgctc atgcaccgta aatatttccc aggcattgcc gtccggatct gtgaaccaga
    55021 gtttatcctg cctggcaaag cagcagacct cgttttcctc gacccgttca aggaggccgt
    55081 gttcctgcag tcgctgcttc catgaccgga tctcttcaat cgaatcgacc tcgatcccga
    55141 aatggttcac cgaactgatt ctgccggtgg acgacaccaa cgaaagattc aacgccggag
    55201 ccgccagatc gaacttggca tagtcggccg tctgtttctg cggcgacagc ccgaatactt
    55261 ctgcatagaa ttcgacggag cgtggcacgc tgcgcacatc gagagaaata tggggacgca
    55321 tggggagctc ctttcttcag catgggcggg aaatccgcct cttacttgta aagacggagc
    55381 accggcaaaa aggacgcagg catgtaccag caccggtttc cccattccct tgacattaaa
    55441 taaggagcaa aaccccatct gcaagtagtg cggagagggg aaagatacac gcaaccatgt
    55501 ggccgacatc gactgagtga tcacaggcac tgagcagctt agcgactcga aactataatt
    55561 gatgggtcaa tgcgtagcgg ataagcgcgg acgtggtttt cagctgcagt ttcgacagca
    55621 gcctcatacg gtaggtgctt acggtcttga cgctgaggtc gagtgcctcg gcaatctccg
    55681 tgaccgactt gccgctgccg atcatcgaca agacctccag ctcacgatca gataacagtg
    55741 tgtgaagggg cctgcccgcc acctgcgccg gagtcgcctc cagccgctcc gccagtgacg
    55801 gggtgacata ctgccggcct gctagcacgg tcttcactgc tgtggccaac tcatgagatg
    55861 cgctgtcttt ggtgacatag gcagcggctc cgcccttcaa ggctcgcagc gcatattgct
    55921 cttcagggtg gaaactcaag accacgacag gaagggctgg acggatcgct ttcaccttct
    55981 tcagaacatc caagccattc gtgtccggaa gattgatgtc aagaatcaac agatcccatg
    56041 gtgcctcttg gacatgccgg atcgcatcgt ggcctgtacc ggcctccgtg atcttgacat
    56101 catcgaatgc atcagcgagc agctgtttga gaccgactcg aacgaccgcg tggtcgtcga
    56161 caaggagaat gttcataggc tcaaggcggc taccctcaag aaatggacca gaagcgatgc
    56221 cggttgattg cttcacgaac atcatgggag taggcggtaa cttattgatc agtcgaactt
    56281 tatctcattg ggcagatgaa gcaattggac gggatccgat cgagtcgtag gataaatcct
    56341 acagcctgcc ccatttcgtt tggtcaacca tgacttgttg aactagacca gcgtgtggtc
    56401 gagagtgtag cgaataagct ccgtggtgtt cttcatgtgc agtttgtcga gcaaccttgt
    56461 ccgataggtg ctgactgttt tgacgctcaa ggataggtct cctgcgatcg ctgacacagt
    56521 tttccccttt cctatggcac agagaacttg gaactcccga tcagagaggc gctcatgcgg
    56581 cagctgctct acaggcgaga aggccacagt cgccaggcgt tcagccaggg tagccgtaat
    56641 gaattttcga ccggccagca cctgtttgat agcggtgact aattcctccg gtgcccgctc
    56701 cttggtcagg tacgcagccg ctcctgcctt gagggcacgg agcgcatatt gttcttcagg
    56761 gtgaaggctc agcatcatga ccggaagcaa cggttggcgc aacttgatgg ctttcaacaa
    56821 ctcgacccca ttctgatcgg gaagattcac atcaagaatg gccaagtccc accggacctt
    56881 atccaaaagc atcagggctt cccgaccgga tccagcctcc catattgtgg catacgggta
    56941 ggcatcctgg acaagctgct tcaacccaat ccggacaatg gcatgatcat caacaatcac
    57001 catattcatg aaaactcctt tgtcccctgc gggaccccga gctacagtgg aaatatcaac
    57061 gtcgcttcag tccccgcacc gggggccccg ataatcgtga cggttccgcc gacggatttc
    57121 acccgttcat acaaccctcg caaccccagc gaatgagtgc cgttccattc acagtcctgg
    57181 atgccccgtc catcatcgca gactcgcaag atcaattgcg aagccgccat cgacagctcc
    57241 accgagaccg ttgatgcctg ggcgtgccgc atcacattcg tcaataactc ctgtgcgctt
    57301 cggaacaccg tcgtggataa ctctccttcg agtgagagcc gctgaatgcc tggatcgacc
    57361 gtcagttcac aaggcagccc cgtccgttca cgaaaggagg aaaccagcca ttcaatagcc
    57421 gctaccagcc ccagatcgtc caggactccg ggccgtaagg cggccgctgt ttctctcaca
    57481 gaccggatca gtccatccac agtatccagc atggcctcaa acttggagct cacatgcgcc
    57541 gattgactct cagatatcat gggcgtcaac cgtttggcca gccaggccac gtcgagtttc
    57601 aagccggtca aggcctgccc aaattcatcg tgcagctcac gcgcaatacg gcgccgctct
    57661 ccttcttccg cctgcgccaa ctgtcgagtg acttcacgta accggtcata ggcggcttgg
    57721 cgctccgttt ccgcccgttt gcgcgcaatg gcggtcgcca atccgttgca gacggactcc
    57781 atcgtttgac gcatcgcttc agagatttcc tcccggccga atgtggcaaa gaccccgtag
    57841 acttgatcct cgacaatcaa cggaaagccg gcgaacgaac gcagtccgtt atccttcaac
    57901 catcccttgt ttggcaatcg ctcatcctga gcgacatcat tcgtaaagag agcccccgct
    57961 ccctgagcga ttcgtccgac cttgagggcc cccatcggaa tacggcggta ctccccgttc
    58021 aggtttgtgg agagaccgga actcgcatgg aggtggaggc attcagtgcg gtcttggcac
    58081 caggaagcct tatggcattc cgcacagaga tcgccgggtt ccctcagcca gatccttgta
    58141 aaagccgctc cgagatgctt cacggccgca tcagtgcaac gctggagttg cgaatcaagc
    58201 ggctcatcgc ggttcagtag aagactgact tcggcggcaa atgctgcgca cttggccccc
    58261 tcttctaagg cctgctctgc gagttttcgc gcggtaatgt cgcgggtgat ggcttcgacg
    58321 ccgatgatcc gcccttccgc cactcgcagc tgcacgcgtt gaccgaacca aacctcgcgg
    58381 ccatccttgg tgatggctgg aaactcaagg taggtgctgg gaatccgctc ggctacttgt
    58441 tgtttataga agcgttgcgc cgcctctcga gcgtccggac gaatgagttc gagataatgt
    58501 ttgcccaaca actcctcttc tcgatacccc atgaccgcac aagcggcggc gttgatgtag
    58561 gtaaaacgcc cgtccattcc tgcggtatag atgacgtcgg tggcctgcga gacgagtgaa
    58621 ctataccgtt cttcactcgc gcgcaaggcc tcttccatca tctgccgctc ggtgatgtcc
    58681 tcggaaattc ctaatagata gcgtggtttt cccgactgat cgtagagtgg aatcttcttc
    58741 gtatggagta tcctggttcc tcgatatcta gtttgaatcg gttccctcgg aatatccttc
    58801 agacgccccg actcaagcac ttcccggtct ttgcgagtga agaagtcggc ttcctcccgc
    58861 ggaaagaaat cataatccgt tttcccgatc aattcgttac ggtggtaccc gagcagatcc
    58921 tccccggctt tattgagctc cacaaaccga agatccttgg cctccttgac aaacagcata
    58981 ttgggaatat tctccagcac cgacagcacg aatgcctgag cttctttgag ttctcgttcc
    59041 gcgcgctttc gcgccgtgat gtcgatctcg atgccggcca tgcgaagcgg cttgccttgc
    59101 gcatcgcgtt tgatcacgcg cccgcgaccc ataatccacg tccactcacc ggcagccgtc
    59161 ttgacccgca tctctgcttc aaaaggctct atcttccctt cgagggagtt ctccaaggct
    59221 tgcatcacat ggtcacgatc ttctggatgc acaccgtcac tccacgacga cacatgaggg
    59281 atcacttcat cgaaagacat tcctcggttc gtggcccagc gctcgttgta ctggaccgcc
    59341 ccggtcgtca catcccagtc ccatgtggcc acacccgtcg aatccaagat gagctctaac
    59401 cgttcctgac tttcccgcaa cgcggccgtg cgttctttca ctcgacgctc cagttcctca
    59461 tgcgcctgct gaagcgctgt ctccgcggcc ttgcgttccg taatgtcccg aacgatcgcg
    59521 caggtatacc cacgccccgc gtgctccaga taattcaagg acacttcgac aggaaagatg
    59581 gtcccgtctt ttcgccgatg ctgggattca tagttgacga cctgaccttg tctgagaagc
    59641 acaagacact gcgcataccg ctccggatca tggtgaggca cgatgtcggc gatcgtgaga
    59701 gctttgattt cgtcggaatt gtaccccagt gaacggcgag ccgcgtcgtt ggcgtagatg
    59761 aggcgctgct tcatgtcggc ccagaagatt ccgtcgccgg catgatcgac agcgaacaac
    59821 gccagtcgca t
//
LOCUS       DATIFR010000115        16480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_74679, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000115 DATIFR010000000
VERSION     DATIFR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74679"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VLD60_06615"
     CDS             <1..1660
                     /locus_tag="VLD60_06615"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSB44664.1"
                     /translation="VVAHENAHMWFGDLVTMSWWNGLWLNEAFATFMEMLAVDDWKPE
                     WKRWDTFSVSRAAAFSVDGLHSTRPIEYPVRAPKDADAMFDVLTYEKGASVLRMLEQH
                     IGPTVFRDGVRAYLRKHAYANADTKDLWVSLGRAANQPVTDLMGGWIFQPGYPLITAE
                     INGQHELVLTQQRFTYLPDPSPGMWHVPIRLRALTDQQRETKRVLLTDTKTKVDLTAN
                     TTYAVINEGGHGFYRVRYGPALMKRLLAAGLDKLAAIERFNLVNDAWATAVAGLMPIT
                     DYLELTGQFVADRDKNVWAILIDSFAFLNRIIDPADRPALEAFVRTRVGPVATEVGWS
                     GKPDESDLTRQLRSELIGALGRLGNDPAIQARAGELYALSKKEPTVVDPNLVPALVSI
                     MAFTGDVARYEEFFERFRKASTPQEERRYLFSLAAFQQPALLDRTLSMTLNGEIRVQD
                     APFMVSAVMTNVYGRQQGWAFVKTNWDQMDRRFPKQGLRRMCGGITGLCTPELEQDVR
                     EFFASRKIDLGGKTLEQYLEQLRITVAFRERNGRDLRQYLRQRL"
     gene            complement(2091..4757)
                     /locus_tag="VLD60_06620"
     CDS             complement(2091..4757)
                     /locus_tag="VLD60_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB44665.1"
                     /translation="MNRFIFAMAWRETRGAWRHFLYFFVCIAVGVGALVGVSLFAAHV
                     DRAVSKEAKGLLGGDLEIRSSHPLSAKSWDVLQSLSERGIVVTHVSELVAMAARADGG
                     AGSGQTTQIVELKAVESSYPFYGTMRLEPARSLEQLLHPDVAVCKGMDCRGAVVQEAL
                     LLRMGLAVGDRLKIGQTTFLITGLVRTEPDRMANAFSLGPRVLVTQEGLQAAELIKPG
                     SRVRERYLVKLPAGMPFESLRAELRGRLASDSVRVSGYRDAQPQLKQFLEQLSRYLGL
                     IGLTALLIGGLGVAMSIQAFLQEKLRTIAILKTIGADSATVVSTYIVQALGLGLLGSL
                     AGMLLGVLLQRVLPPLLAGMFVSDLLDQLGASAELSAISIGPLLKGGALGLFATLLFT
                     IWPLLRIREIKPAAIFRQEVESAPASSTVRRAPWWVRWGLNDRLTILTSVSVAIGLGG
                     LSIWQAGSWKVGGLYFGGLSLAVVMLMVGARAFVGVLRLLPRLQSLSLRYAMGNILRP
                     GSHTSGMVVAIGISMTIIVTVSLVEQELLHEVGETRPVDAPTFFFIDLQPDQTEGFAK
                     LVQKQTAGRAPELTPLVRSRLHAINGEAVKVEEEREQDERREENKEERRKQWYLSREY
                     VLTFLDQLPKGNEIIRGAWWKPGEAFPIPQVSIEEEAAKSLGLEIGSRVTFDIQGATI
                     DAEVASIRKVEWGNFSTNFYMILSPGSLDGAPLTYVGTIHVPPEQEVPLQQAVVASFP
                     NVSAIHIGDVLENFARVLDRLALAIRAVALFCVVTGGLVMAAALAATRYRRLYESVIL
                     KALGATRGLIGRTFAAEYALLGMVGGVIAVGLSSALSWLVLTYIFDLSWSPQPVVLLR
                     GLALTVLLTLLVGWLSTYQILGQRPLAILRHE"
     gene            complement(4754..5458)
                     /locus_tag="VLD60_06625"
     CDS             complement(4754..5458)
                     /locus_tag="VLD60_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44666.1"
                     /translation="MITIQHLSMRLSAGGRSVTILDDVTLEIPAKQMVAIVGPSGSGK
                     STLLGLIAGLDRPTSGAIILNGLDITQMSEGPLARYRRAQVGYIFQSFHLIPTLTALE
                     NVLVPLELAGTDSPTDQAIELLRAVGLGDRVHHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSVTGQQVMDLLLSLHRDYGTTLVLVTHDRAIASSMHRVIALRDGRVE
                     SDDIIGPSTKIPERIR"
     gene            5493..6194
                     /locus_tag="VLD60_06630"
     CDS             5493..6194
                     /locus_tag="VLD60_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HSB44667.1"
                     /translation="MVLYCSPMKVPRVIRAVPAAVIALIIQGLTLSNSTIATPWTSNN
                     SSVADRPRIVAFGDSLTAGLGVSAEESYPAQLQRRLDGAGLRYRVVNAGVSGDTTAGG
                     LRRVAWVLNSKPRIVILELGANDGLRGLSLRETRGNLERIIRQLQAASVTVVLAGMKL
                     PPNYGTEYTAGFETIYRSLAQKYHLTLIPFFLDGVAGSASLNQADGIHPTGEGYQIVA
                     DRVLKVVKPLLEQKQ"
     gene            complement(6291..6485)
                     /locus_tag="VLD60_06635"
     CDS             complement(6291..6485)
                     /locus_tag="VLD60_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44668.1"
                     /translation="MRKMLAQVGAFAGTLVLVGASQVLANEPKGEGAPDYSGITGMYY
                     ALIGLILAYGVYDTFFKKSH"
     gene            complement(6596..7489)
                     /locus_tag="VLD60_06640"
     CDS             complement(6596..7489)
                     /locus_tag="VLD60_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44669.1"
                     /translation="MRNKIPCSADRLSLLDPPMIQGVVDRNAFHLGNQYLTENRVRII
                     EADEAQISSAVIGNSGLYEQTIRLKDGHLVSKCSCSLPEEPMCRHCIAVLLEYHRWVQ
                     PRGNVKRTNQASPPAAPQAGSNNGNGAPSSSPGADLKLSEVMAFIEWLQPAVKSLEQG
                     QPLPDSSRLAGDIGAWVQAMRNLDERRRESEEIQLRLESEQKDREAYVQRMTQQLQAS
                     MEEVKSAQSNLQQLQQEIALYKDMFVKVTEIASEVGGYDSQIKTIAGELLSKGSQLDK
                     LANSFREVAMALKTITKSGNS"
     gene            complement(7675..8694)
                     /locus_tag="VLD60_06645"
     CDS             complement(7675..8694)
                     /locus_tag="VLD60_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HSB44670.1"
                     /translation="MRSDQVSDAGSRGRGLWSLFLIALLLSAQACADGGISASRSAAA
                     IAPAEITEGQIIETATGRPVSMDEVMDRVRQQEVVYLGEEHHNGFHIEHALVLLSRLT
                     SESRRPVLAMEMFGWESQALIDRYVGGEELSQQEFLDQVGWQQNWGGPFENYEPLVSY
                     AKTRHLHLAALNPPKQLVRSVARNGLDQTRREPDWARWGLHEETIIDDPLYRERILQQ
                     LRACHDGGSDKLYESMYEASMVRDEGMARTVVSLVESIRAAKDPLAGPVVSYTGGGHI
                     QYNLPIAKRVARRLSQEVRQVSIYMTSYERGRLRDLQEMIEGRIADYVWLTPLGSQGT
                     PRRCR"
     gene            8826..10142
                     /locus_tag="VLD60_06650"
     CDS             8826..10142
                     /locus_tag="VLD60_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44671.1"
                     /translation="MTSDAQLLADFRSQVTKLLQERDSEWEASRRLVEASQLATTLKR
                     LIEEARRIDLPQQVRESIIVALKQGEANRIQDLPGPRLKELTGLPPTKAIRALCVWFD
                     VIENPPSRWTVSSLSSQAIDTFVRIHQNPFDLLLSSDVASVLDLGAGDLSFAEELAIQ
                     YAPKIHELGRTLTLHALDRLNPQSRLGGALHPERARLQMLRSRSDLSFRFFGDQDMGA
                     LEPLDRAGQLAPCYTVVTCWAPATPTFAYEPTRLSTDVIQKELQRTKGSFHQTRYAGE
                     PALEVQHGGRALLFPPWKFEIRGPLLLLDILARRGQLCLLAAVDNQVFWELLAQLLDD
                     PRYRPENQPFTSDNIPTIFGSTFEGLIRLQIGESVNLADCGTLRSEIPRALPMTSQYS
                     SPYRFSSIQVRRGAFFPNIPASSTARKFRDMVEEAPPWMVTLVPAR"
     gene            10276..11217
                     /locus_tag="VLD60_06655"
     CDS             10276..11217
                     /locus_tag="VLD60_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSB44672.1"
                     /translation="MNTSQTIRAIQDNIALVIKGKTHVIEMAVICLLARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSMFNRQKQAFEFMPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQISVDNQTHPLQQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPSLEEERKVLERPYALHPAEMLEAVLSEEEVLELQHRVDQIHMDE
                     SLTEYLLAIVQATRQSELLSLGVSTRGALALNKTAKALALVRGRDYCLPDDIKELAPT
                     VLSHRVMLSRAQGMRGQSFEQAERIILDIVDTVSVPV"
     gene            11240..12388
                     /locus_tag="VLD60_06660"
     CDS             11240..12388
                     /locus_tag="VLD60_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSB44673.1"
                     /translation="MSNPSRHTPIGQLASRIRHLATRRSIRLTSEGTRFLLFTIGIGI
                     AAINTGNNLFYLLLAMMFSLVVISGLLSELCLRCLDLHRHVPDLITAHEPTTVTLSVT
                     NRNSRFPTFSLRLFDVVGGQDVDRGLFVRYLPAQRSLLLSYPLLAAKRGWLHLDYIRA
                     ETLFPFGLFLKRALYPVEANILVGPPIKPLSLRFIDELVCEGQGLALPRRGHGTQLYN
                     LRLYQPGDDSRSIHWMTTARTSQLVVRETEAEDQRRMTILLSTTAPEECDSLFEQSVT
                     LVASLLWQLSTRSYPLRLIVGQEDAGLGFGSDHLLTMLRLLALCERQNPGRPEANIAA
                     SASALSRDTERGYTLAILPWSGEDVTAVTVTADRVLHAAQIEEVTHAF"
     gene            12378..14564
                     /locus_tag="VLD60_06665"
     CDS             12378..14564
                     /locus_tag="VLD60_06665"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HSB44674.1"
                     /translation="MPFEQAFRLSSILLTATAFAGLVFARTVPVWLAVPITIILLIIL
                     LQTMGWAYGRRITERISASSIMWNVLLVTAFGFFLLNVTAISRELLPAGIHFLIMLLG
                     IKLFTLQQRRDYRHLYAISLMAILASAALTTDIGYVPIFLCYLLTVVWSLLLYHLTNE
                     TSVHAATPTGERASVAPSAGHVTGGFFWLTNGIAVITFTLTLALFFLLPRVSAGLLQK
                     SRGEGLKTTGFSERVDLGMIGSVKEDPQIVMRVELPDQPAAGKERLYLRGLAYDRYNG
                     SSWSTSSRERRNLGLIADGIFAVRSSGTRTPSSISEPLRQDILLEALDTSVLFAAPFA
                     EFISGEFSGMQVDGMTGLHLPFPASSRIRYSVTSREHHIISDEQLASGLDYSNSIRDR
                     YLQLPELSMQVIDLAQQVTGSAPTPYEKTVAIQQHLLNSYRYSLDTGTTHSTRPIEDF
                     LFTRKTGYCEHYATAMVLLLRSLGIPARLVTGFLATEWNDFGNYFTVRQRDAHAWVEV
                     YYPHSGWITMDPTPASTAASSPSGWEALQRIGESLRLQWDRVFIRYSARDQLAVVYSL
                     RDSSDSARDYLSRWMITLKTTVSQTLGQIKAHAKSASPLALWLAAILPGVGLALLFVV
                     IKKTWWDTIPSHRPTIRKQEYIAQLYKKMLDVAAKQGIIKHPTLTPMEFVQLVRREWS
                     EAGSMVADLTMLYCRGRFSGSPLTHEELSLAGEKIRALQRMSRATG"
     gene            complement(14584..15543)
                     /locus_tag="VLD60_06670"
     CDS             complement(14584..15543)
                     /locus_tag="VLD60_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSB44675.1"
                     /translation="MNDVTYSLIEQAVDVLRGVALVTPVKSNRALDERAAASVFFKCE
                     NFQRVGAFKFRGAYHAIARHMASHHTSTFATISSGNHAQGLALACRILGAKAHVVMPN
                     PHSSMKRQAALDYGAAVCVAEDRTRAEAMLREMMKDTGAMLVHPFNDPLVIAGQGTIM
                     VELLDQVNDLDIVLAPVGGGGLLSGLCIAAGACRPQIKIFACEPAGALDAIESVRQNR
                     VVPMSNPNTIADGLRTSLGDRTLPVLRRYLAGFFIVEETEILQAMRFAQEQLKLVIEP
                     SSAVALAPLLRREPQLRGKRVAVVLTGGNVDLAPLRQNLDALA"
     gene            complement(15638..15712)
                     /locus_tag="VLD60_06675"
     tRNA            complement(15638..15712)
                     /locus_tag="VLD60_06675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15678..15680),aa:Gly,seq:gcc)
     gene            16247..>16480
                     /locus_tag="VLD60_06680"
     CDS             16247..>16480
                     /locus_tag="VLD60_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSB44676.1"
                     /translation="MFVSRRQFLKVSAGTVAAVAVADKVLALTALQPVIEVGNPLGDY
                     PDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIR"
BASE COUNT         3591 a         5018 c         4368 g         3503 t
ORIGIN      
        1 cgtcgtggct catgaaaacg cccatatgtg gttcggcgat ctcgtaacca tgtcctggtg
       61 gaacggcctc tggctcaacg aagccttcgc tacgttcatg gagatgctgg cagtcgacga
      121 ctggaaaccg gaatggaagc ggtgggatac gttcagcgtg tcacgcgccg ccgccttctc
      181 agtcgacggt ctccacagta ccaggcccat cgaatacccg gtccgcgcgc cgaaagacgc
      241 cgatgccatg ttcgacgttc tcacgtatga gaaaggcgcc tcagttctgc gcatgctgga
      301 gcaacacatt gggccgaccg tctttcgcga tggagtccgc gcctatctaa gaaagcacgc
      361 gtacgccaat gccgacacga aagatctctg ggtctcgctt ggaagggcag ccaaccagcc
      421 ggttactgac ttgatgggcg gctggatttt tcagccgggc taccccctca tcacagctga
      481 gatcaatgga cagcatgaac tggtcttgac tcaacaacgg ttcacctatc ttcctgatcc
      541 gtcacccggc atgtggcacg ttccgattcg actgcgcgcg ctaacggacc agcagcgtga
      601 aaccaagcgc gtgctgctca ccgatacgaa gaccaaagtc gatctcaccg ccaacaccac
      661 gtatgcggtc atcaatgaag gcggtcatgg cttttatcgg gtacgttacg gaccggcatt
      721 aatgaaacgc ctgctcgccg ccggcctcga caagctggcc gcgattgagc ggttcaatct
      781 ggtcaacgat gcgtgggcca cggcggtcgc gggcttgatg ccgatcacgg actatcttga
      841 gctgaccgga caattcgttg cggaccgcga caagaatgtc tgggccatcc tgatcgactc
      901 gttcgcgttc ttgaatcgaa tcatcgaccc agccgaccga ccggcactcg aagcctttgt
      961 ccgcaccaga gtcggcccgg tcgccacgga ggtcggatgg agcgggaaac cggacgaaag
     1021 cgatctcacg cgtcagctcc ggagtgaact gatcggtgca ttgggccggc tcgggaacga
     1081 cccggcaatc caagcccgtg ccggagaact ctacgcacta tcgaaaaaag agccgacggt
     1141 cgtcgatccc aacctcgttc ctgcgcttgt ctcgatcatg gcctttacag gagacgtcgc
     1201 caggtacgag gaattctttg aacgattccg caaggcttcg acaccccagg aggaacgacg
     1261 ctatctgttc tcattggcgg cattccaaca accggcgctg ctcgaccgga ccctctccat
     1321 gacgctcaac ggcgagatcc gcgtgcagga cgcaccgttc atggtcagtg ccgtcatgac
     1381 caacgtctat ggccgccagc agggctgggc ctttgtaaag accaactggg atcagatgga
     1441 tcgacggttt cccaaacagg gtctccgccg catgtgcggc gggatcactg gattgtgcac
     1501 gccggaactc gaacaggacg tccgggaatt cttcgcttcc cgcaagatcg atttaggcgg
     1561 gaaaacactg gagcagtatt tggagcaatt gcgaatcaca gttgcattcc gcgagcgaaa
     1621 cggtcgcgac ttgcggcaat acctccggca acgcctctag cgacgggtaa gcttccgttc
     1681 tctccttcct ccggcaaacg accgtcaacc tggcttccga aggccacgcc ctgacgctcc
     1741 gtcaggagac acaacgccga tagcctcccg ggcgctgccc agcttgagag aacgacgacg
     1801 gagcccgcgc gatcaccggc ggatatctgt accgcggtca ggcgaaccta tccatcaatg
     1861 ggacttattt ctatgcagac ttttaccccg gatctgtttg gagctttcac ctgctaggca
     1921 gatagcccgc acagaggtca gctggccatt gctcagccag tcaagaggtc aggtgaccag
     1981 cttcggcagg aggcacgggg tgaactctat ctgatgaccc aggggagcaa tccgcttcgc
     2041 atcgtgcaaa actagattcc cgatatgcat gaacccccta ttcccaggtg ctactcgtgc
     2101 cgaaggatcg ccaatggacg ctgccccagg atctggtagg tactgagcca ccccacaagc
     2161 agcgtcagga gaactgtcag cgcaaggcct ctgaggagga cgacgggctg cggactccac
     2221 gacagatcaa agatgtaggt caggaccaac caggacaatg cactcgagag tccgactgca
     2281 atcactccac cgaccattcc caagagcgca tattctgcgg caaatgtcct cccgataagc
     2341 ccccgcgtgg cgcccaacgc cttgagaatc acggattcat acaaccgtcg atatctggtt
     2401 gccgccaatg ccgccgccat taccaatcct ccggtcacca cgcaaaagag ggcgacggcg
     2461 cgaatcgcca gcgcaagacg atccaggacc ctggcaaaat tctccagcac gtccccgata
     2521 tgaattgcgc tcacgtttgg gaaggacgca actaccgcct gctgcaaggg cacctcctgc
     2581 tcaggcggca catgaattgt gccaacatag gtcaatggag cgccgtccag cgaaccggga
     2641 gacaggatca tatagaagtt ggtcgaaaag tttccccatt caaccttgcg gatgcttgcc
     2701 acctccgcat caatcgtggc gccttgaata tcaaacgtca cccgggagcc gatctccagc
     2761 cctaacgact tggcggcttc ctcctcgatc gagacctgcg ggatggggaa tgcctctccc
     2821 ggcttccacc aggctcctcg gataatctca ttgcctttgg gcagctgatc gagaaacgtc
     2881 aggacgtatt cgcgcgacag ataccactgt ttccgccgct cttccttgtt ctcctcccgc
     2941 cgctcatctt gctcccgttc ctcctcgact ttgaccgctt ctccgttgat cgcatgcagt
     3001 cgagatcgca ccaatggcgt cagctccgga gcacgaccgg ccgtctgttt ctggaccagc
     3061 ttcgcaaagc cctccgtttg atccggctgg agatcgatga aaaaaaaggt gggcgcatcc
     3121 accggacgcg tctctccgac ttcatgaagc agctcttgct ccaccagcga caccgtgacg
     3181 ataatcgtca tactgatccc gatcgccacc accattccgc tcgtatgact gccaggacgc
     3241 aggatgttgc ccatggcata gcggagactc aacgactgca gcctgggtag caggcgtagg
     3301 actccaacga atgctcgcgc gccaaccatg agcatcacca cggccagact caggccgccg
     3361 aaatacaagc cgccgacctt ccatgaacct gcttgccaga tcgaaagccc tcccagacca
     3421 atcgcaactg agaccgatgt caggatagta agacggtcgt tcaaccccca tcgcacccac
     3481 cacggtgctc tccgaacggt ggaactcgcc ggagccgact ccacctcctg ccgaaagatg
     3541 gcagcaggct tgatctcgcg gatcctcagc aatggccaga tggtgaagag taacgtcgca
     3601 aagagcccga gcgcgccgcc tttgagcagc gggccaatcg aaatagccga caactctgct
     3661 gaagccccta attgatcgag taagtcagag acgaacattc cggccagtag cggagggagg
     3721 acacgttgaa gcaagacccc gagtagcata ccggccaagc tgcccaatag accgagtccc
     3781 aacgcctgta cgatataggt ggaaaccacg gtcgccgagt ccgctccgat cgttttcaaa
     3841 atggcaatgg tcctcagctt ttcctgcagg aaagcctgaa tcgacatggc gacgccgagt
     3901 cctccgataa ggagagccgt cagtccgatc aggccgaggt aacgggacaa ttgttccagg
     3961 aactgtttca gctgaggttg cgcatcacga tagccggaca cgcggacgct gtccgaggcc
     4021 aaacgtcccc gtaactcggc ccgcagtgat tcgaacggca ttcccgccgg aagcttgacg
     4081 agatagcgct cgcgcacgcg gcttcccggt ttgatcagct cggcggcttg cagcccctcc
     4141 tgcgtgacaa gaacacgggg acctaaactg aatgcattgg ccatgcgatc aggttctgtt
     4201 cggacgaggc cagtaatgag aaaggtcgtc tgcccgatct tcaaccgatc accgacagca
     4261 aggcccatgc gtagaagcaa ggcttcctga accacggccc cgcgacaatc cattcccttg
     4321 cagacggcga catcaggatg cagcaattgt tctaaagatc gagccggctc cagcctcatg
     4381 gttccgtaga aggggtagga cgattccacc gccttgagtt ctacaatctg ggtcgtctga
     4441 ccggatcccg ctccaccatc cgcccgagcc gccatcgcca ccagttcgct gacgtgagtg
     4501 acgacgatcc cgcgctcgct cagagattgc aggacatccc agcttttcgc actcagggga
     4561 tgagatgagc ggatttccag atcccctccg aggagacctt tggcttcctt gctcacggca
     4621 cgatctacgt gagcggcaaa gagcgagacg ccaaccagcg cgccaacccc caccgcaata
     4681 cagacaaaga agtacagaaa atggcgccaa gcccctctcg tctcgcgcca agccattgcg
     4741 aagatgaatc ggttcaccgg attcgctctg gaatcttcgt ggaaggtcca atgatgtcgt
     4801 cggactcgac tcgcccatcg cgtaaggcga tgacgcgatg catcgatgag gcgatggcac
     4861 ggtcgtgcgt gaccaggacc agcgtcgtcc cgtagtctcg atggagggag agcagcaggt
     4921 ccatgacctg ttgcccggtc acggaatcca agtttcctgt cggctcgtcg gcgaggagaa
     4981 tcggtgggcg acaggcgaag gccctggcca ccgcgacgcg ttgctgttca ccaccggaga
     5041 gttggactgg atagtgatgg actcgatctc ccagccccac agcgcgcagc agctcgatcg
     5101 cctggtccgt gggcgaatcg gtaccggcca actctaatgg gacgagcacg ttctccaatg
     5161 ccgtcaacgt ggggattaaa tggaaggatt ggaaaatata cccgacctgc gcgcggcgat
     5221 agcgggcgag aggcccttcg gacatttgtg tgatatcgag gccgttcaat atgatcgctc
     5281 ccgatgtggg acgatcgagg ccagcgatca gcccgagcaa cgtcgatttc ccgcttccgg
     5341 acggtccgac gatggcgacc atctgtttgg ctggaatttc aagtgttacg tcgtcgagga
     5401 tggtgaccga gcgcccgcca gcggacaaac gcatcgagag atgttggatc gtgatcatcc
     5461 gatctggttc cccataaccc agaatgtcgt cgatggtatt atactgtagt ccaatgaaag
     5521 tcccccgcgt tattagagcc gtgcctgcag cagttatcgc cctgatcatt cagggattaa
     5581 ccctatccaa ctctactatt gccaccccct ggacatcgaa caattcctcc gttgcagatc
     5641 gtccacgcat cgtggcgttc ggcgacagcc tcacggctgg tctcggcgtt tccgcagagg
     5701 agtcttatcc tgctcaatta caacgtcggc tggacggcgc ggggctccgt tatcgtgtgg
     5761 tcaatgcggg cgtgagcgga gataccacag ctggcggact ccggcgcgtg gcctgggttc
     5821 tgaacagcaa gcctcggatt gttattcttg aactgggcgc aaacgatgga ctccgtggtc
     5881 taagcctccg ggaaacgagg gggaaccttg agcgcatcat ccggcagctc caggcagcct
     5941 cggtcacggt agtcctcgcc ggcatgaaat tgccgccgaa ttatggcacg gagtatacag
     6001 ccggattcga aactatttat cgttcactcg ctcaaaaata tcatctgacg ctgatcccct
     6061 ttttcctcga tggcgtggcg ggatcggcct cactcaatca agctgatggg attcatccaa
     6121 cgggagaggg atatcagatt gtcgcggata gggtgctgaa ggtcgtgaag cccttactgg
     6181 agcagaagca ataagtgatg gcgtgatcga tcgcaggaag aaacaggaaa tccatgggcc
     6241 ccaatgaagt tcttcattac ggcccatgga atctgatcga taagaaaacg ttagtgggat
     6301 ttcttgaaga acgtgtcgta gacgccgtac gccaatatca atccgatcag cgcgtaatac
     6361 atgccggtaa tcccgctgta gtccggcgcc ccttcgccct ttggttcatt ggccagaacc
     6421 tgcgaggcac cgaccagcac cagggttcct gcaaaagccc caacctgcgc aagcattttt
     6481 ctcatgtgcg atcctccctc aagtcaaata aagaaaaagt gggccgcatt ctacagaaat
     6541 cgcccgagag gacgcaagag ggaatctgat tgcgcaggat tgacgcgagg ggggattacg
     6601 agtttccgga tttcgtgata gttttcaaag ccattgcgac ttctctgaac gaatttgcga
     6661 gcttatcgag ctgagagcct ttagacagca gttctcccgc gatggtcttg atctgactgt
     6721 cgtagccgcc cacttccgac gcgatctccg ttacctttac gaacatgtct ttgtagagcg
     6781 ctatctcttg ctgaagttgc tgcagattgg attgagctga ttttacttct tccatcgaag
     6841 cttgcagttg ctgcgtcatc cgttgaacat aggcctcgcg atctttctgc tctgattcga
     6901 gcctcaactg gatttcttcg ctttctcggc gacgttcatc caaattccgc atggcctgga
     6961 cccatgcgcc aatgtctccg gctaatcgag aagagtccgg caacggttgg ccctgttcca
     7021 gcgacttcac cgccggctgc agccattcga taaacgccat gacttcactt aatttcaagt
     7081 cggcaccagg actggacgat ggggcgccgt ttccattgtt gctaccggcc tggggagcag
     7141 caggaggaga tgcctgattg gttcttttta cattccccct tggctgaacc cagcgatgat
     7201 attccaagag cacggctatg caatgccggc acataggctc ttccggaagg gagcaactgc
     7261 acttggagac taaatgaccg tccttcagcc gaatcgtctg ttcatagaga ccggagttcc
     7321 cgatgacggc ggatgaaatc tgcgcctcat cagcctcgat aatgcggacc cggttttccg
     7381 tgaggtactg gttccccaaa tggaacgcgt ttcgatccac gacaccctga atcatcggag
     7441 gatccaacag actcagtcgg tcggccgaac aaggaatttt gtttcgcata cgcaggtttc
     7501 ccaccgtaaa cgtcggttct ccggcctctg atgttgtgct agtgtgaaac gcctgcctga
     7561 aagtgtcaag ctgtcatcgg ttatttacag tccacagggg gagcctgagc agttggcgag
     7621 gatgcgggat catgcgggcg ctggaaaggg tcatcccggc gagaaaagcg gttgctaccg
     7681 acatcgtcga ggtgttccct gcgaacctag aggtgtgagc cagacataat cagcgatgcg
     7741 cccctctatc atttcctgga gatcgcggag acgacctcgt tcatacgacg tcatataaat
     7801 actcacctgt cgcacctctt gcgataacct tcgcgcaaca cgtttggcga tcgggagatt
     7861 gtattgaatg tgcccgccgc ccgtataact cacgaccggc ccggccagcg gatcctttgc
     7921 cgcgcgaatc gactcgacca gagagaccac cgttctcgcc atgccttcgt ctcgcaccat
     7981 cgaagcttca tacatcgact catacagctt gtccgagccc ccgtcatgac aggcacggag
     8041 ttgctgtaga atcctttcgc gatagagggg atcatcgatg atcgtttcct cgtgcaagcc
     8101 ccaacgagcc cagtcaggtt ctcgacgggt ctgatcaagc ccgtttctgg ctaccgacct
     8161 gaccaactgc tttggaggat tcaatgctgc caggtgcaaa tgccgggtct tggcgtacga
     8221 gacaagcggt tcataattct cgaacgggcc gccccaattt tgttgccatc cgacctgatc
     8281 cagaaactcc tgctggctca gctcttctcc ccctacgtag cgatctatca gcgcttggct
     8341 ctcccacccg aacatctcca tcgcgagaac gggccggcgg gattccgagg tgagtcgact
     8401 caagaggacg agcgcatgct cgatatgaaa cccattatga tgttcctctc caagataaac
     8461 aacttcctgc tgcctgacgc gatccatcac ctcgtccata gaaaccggtc ggcccgtcgc
     8521 cgtctcaatg atctgtcctt cggttatttc cgcaggggcg atcgcggcag cagagcgtga
     8581 tgcggaaatc ccgccgtctg cacaggcctg agcactcaac aacagcgcga tcaagaaaag
     8641 cgaccagaga cctcttcccc ttgaacccgc atccgatacc tgatctgagc gcatgctctc
     8701 tcctaacaca gttggcctcc atcggcaaaa cgactgacct tgaccctctt ccaggaggtc
     8761 gttatgctgc aacacaggtt tctgactccc tagtccgcgc aatcgcccgg tatatccagc
     8821 gatccatgac atctgatgcc caactccttg cagattttcg atcgcaagtc actaagctct
     8881 tgcaggaacg agacagcgaa tgggaggcgt cacgccgttt ggtcgaggca agtcagcttg
     8941 cgacgaccct gaaacggttg atcgaggaag cacggcgaat cgaccttcct cagcaagttc
     9001 gagaatccat catcgtcgca ttgaaacaag gcgaggccaa ccggatccaa gatttgccgg
     9061 gtccccggtt gaaggaactc acaggccttc cgccgaccaa ggccattcgg gcactctgcg
     9121 tctggttcga tgtgatagag aatcctccct cgcgttggac ggtatcttct ctgagtagtc
     9181 aagcgatcga cacgttcgtc cgcattcacc agaacccgtt tgacctctta ctttcatcgg
     9241 atgtggcatc ggtcctcgac ctcggcgccg gcgatctgtc tttcgccgaa gagctggcca
     9301 tccagtatgc gcccaaaatt cacgagctgg gtcggaccct gacgctccac gcactcgatc
     9361 ggttgaatcc tcaatcgcga ttgggggggg cgttgcatcc tgagcgagcg cgactccaga
     9421 tgctccgctc gcgttccgat ctttcgtttc gatttttcgg agatcaagac atgggtgcgc
     9481 tcgaaccact cgatagagcc ggtcaactcg cgccatgcta tacggttgta acctgctggg
     9541 cccccgccac tccgaccttc gcatacgagc cgacacgtct ctcgacggat gtcatccaaa
     9601 aggagctgca acgaacgaag ggctcgtttc accagacacg atacgccggt gagccggcgc
     9661 ttgaagtgca gcacggaggc cgtgctctgc tcttcccccc atggaaattt gaaatcagag
     9721 gtcctctcct gcttctcgat atcctcgctc ggcgtggtca gctctgcctc cttgccgcag
     9781 tcgacaacca ggtgttttgg gaactactgg cgcagctcct tgatgaccct cgctaccgtc
     9841 ccgagaatca accttttacc tcagacaata ttccgaccat cttcggatca accttcgaag
     9901 gcttgattcg tctgcaaatc ggcgaatccg tcaatctggc agattgcgga accttacgct
     9961 cggagatccc ccgcgccctg ccaatgacat cgcaatattc gtctccttat cgcttcagct
    10021 cgatacaggt gcggcgagga gctttcttcc ccaacatccc ggccagcagc acggcgagga
    10081 aatttcgcga catggttgag gaagcgcccc catggatggt cacgcttgtt ccagctcgct
    10141 agaatccctg gcgcattccc ctaccgtcgc aggcgcaaaa ccgcagataa attgaccgtt
    10201 accagaccct ttgctagact tcagcggtgt cacatcacca ctgatttcta agccaccaga
    10261 gccaataatc cagacatgaa cacatctcag acgatccggg cgatccagga caatattgca
    10321 ttggttatca aggggaaaac ccatgtcatc gagatggcgg tcatctgttt gctggcccgt
    10381 ggccacctcc tgcttgaaga tgtcccaggg gtcgggaaaa ccactctcgc ccatagcctg
    10441 gcacggtcac ttgattgctc ctttaaacgt atccagttca ccagcgactt gctgccctcc
    10501 gatattgtgg gcgtttcaat gtttaaccgc caaaagcaag ccttcgaatt catgccgggg
    10561 cccatcttcg ccaacatcgt gctggccgac gaaatcaacc gtacaacccc caaaacacag
    10621 agcagtctgc tggaggccat gagcgaagcg cagatatccg tcgacaatca gactcaccca
    10681 ctccagcagc cgttcatggt catcgccaca cagaaccccg ctgagtacca tggaacgttt
    10741 cctctgccgg aatcccagct cgaccggttt ctgatgcgcc tgcgcatcgg ctatccctca
    10801 ttggaagaag aacgcaaggt cctcgagcgt ccctacgccc ttcacccagc cgaaatgctc
    10861 gaggccgtac tctccgaaga agaggtgttg gaactccaac atcgggtcga ccagatccat
    10921 atggatgaaa gcctgaccga gtacctcttg gcgatcgtcc aagccaccag acagagcgag
    10981 ctgttgtctt tgggtgtcag cacgagaggg gctcttgcgt tgaataagac cgccaaagcc
    11041 ttggctttgg ttcgaggtcg tgactattgt ctccccgacg acattaaaga gttggcgccg
    11101 actgtgttgt cccatcgcgt gatgctgagt cgggcgcaag gcatgcgggg gcagagcttc
    11161 gagcaggccg agcgcattat cctcgacatc gtcgataccg tttccgtccc cgtctgaccc
    11221 gaacgggata ggcaggcccg tgagcaatcc ttcaaggcat accccgatag gccagcttgc
    11281 ctcacgcatt cgccacctcg caacccgccg ctcaatccgc ctgacctccg aaggcacccg
    11341 ctttctcctt ttcaccatcg ggatcggcat cgcagccatc aataccggca acaacctctt
    11401 ctacctcttg ttggcaatga tgttcagcct cgtggtgatc tcgggattgc tctcggagct
    11461 ctgtttgcga tgcctcgatt tgcaccggca tgtgccggat ctcatcacgg cgcacgaacc
    11521 gaccacggtg acattgtcgg tgacgaaccg caacagccgt ttccccacct tttctctacg
    11581 actcttcgac gtggttggcg gacaggatgt cgatcgtggc ctcttcgtca gatatctacc
    11641 cgcacaacgt tcgttactcc tctcctaccc gctgttggcc gccaagcgcg gctggcttca
    11701 cctcgattac atcagggccg aaacactgtt ccccttcgga ttgttcctta aacgagcgct
    11761 ctaccccgtt gaggcgaaca ttctggtcgg cccgcccatc aaaccgcttt cactgcgatt
    11821 tattgacgag ctggtctgtg aggggcaggg cctcgcgctc ccccggcggg gtcatgggac
    11881 ccagctctat aacctgcgcc tctaccagcc aggagatgat tcgcggtcga ttcattggat
    11941 gacgacagcc cgaacttctc agctcgtcgt gcgagagacc gaggccgagg accaacgccg
    12001 catgacgatc cttctctcaa caacggcccc cgaagagtgc gatagcttgt ttgaacaaag
    12061 tgtcactttg gtcgcgtctc tgctgtggca actgtcaaca cgatcctacc ccctccgctt
    12121 gatcgtaggc caagaagatg ccggtcttgg cttcggctcc gatcatctcc tgacgatgct
    12181 gcgcctgctc gcgctctgtg agcgccaaaa tccgggaagg cctgaagcga acatagccgc
    12241 ttccgcctcc gcactctccc gcgacaccga gcgcggctac acccttgcca tccttccctg
    12301 gtcgggcgag gacgttaccg ccgtgacagt cacagccgat cgcgtgttgc atgctgccca
    12361 gatcgaggaa gtgacccatg ccttttgagc aggcgtttag actcagctcc atcctgttaa
    12421 ccgcgacggc tttcgcgggg ctcgtgttcg cccggacagt cccagtctgg ctcgcggtcc
    12481 cgatcaccat catcttgctg ataattctgc ttcagaccat gggatgggcc tatggacgtc
    12541 ggatcacgga gaggatctcc gcctcttcga tcatgtggaa tgtcctgttg gtcaccgcct
    12601 ttggtttctt cttactcaat gtgacagcca tttcgcgcga gttgcttcct gccggcatcc
    12661 acttcctcat catgctgctg ggcatcaagc tctttacact ccagcaacgc cgcgactatc
    12721 ggcatctcta cgccatcagt ctcatggcga ttctggcctc tgcggccttg accaccgata
    12781 tcgggtatgt cccgattttt ctgtgctatc tcctgaccgt tgtttggagc ctactgctct
    12841 atcacctgac caacgagaca tccgtgcatg ctgctacgcc aacaggggaa cgcgcatcgg
    12901 ttgcgccctc cgcgggtcat gtcaccggcg gtttcttctg gctcacgaac gggatcgccg
    12961 tcatcacctt taccttgacg ctcgccctgt tcttcttgct cccccgcgtc agcgcgggct
    13021 tattacaaaa atcccgtgga gaaggattga aaacgacagg gttctccgaa cgtgtcgatc
    13081 tcgggatgat cggctccgtc aaggaagacc ctcagatcgt catgcgcgtt gaactccccg
    13141 atcagccggc agccggaaaa gaacgcctct atcttcgggg tctggcgtac gaccgctata
    13201 atggctcgtc ctggagtacc agcagccgag agcggcgaaa tctcggcctc attgccgatg
    13261 gaatctttgc cgttcgatcg agcggaaccc ggacgccatc cagcatttcc gagcccctac
    13321 gccaagacat cctgttggaa gccctggata cctcggtcct cttcgccgct cccttcgcag
    13381 aattcatcag tggagagttt tccggcatgc aagtcgacgg catgaccgga ttgcacttgc
    13441 cctttcccgc atcttcgcgc attcgttatt ccgttacatc gcgtgaacac cacatcatca
    13501 gcgacgaaca gctcgcatct ggcttggatt actcgaactc cattcgcgac cggtacctgc
    13561 agcttccgga actgtccatg caggtgatcg acttggccca gcaagtcacc ggatctgccc
    13621 caacgccgta cgaaaagacc gtcgcaatcc agcagcacct gctcaacagc taccgataca
    13681 gcctcgacac agggacaacg cattcgacaa gacccatcga ggattttctc ttcaccagga
    13741 agaccggtta ttgcgaacat tacgccaccg cgatggtcct cttgttgagg agtctcggca
    13801 ttcctgcccg actcgtgaca ggatttctcg cgaccgaatg gaacgatttc ggaaattact
    13861 ttacggtccg acaacgggac gctcatgcct gggtcgaagt ctactatcca cattccggat
    13921 ggattacgat ggatcccacc cccgcttcga ccgccgcctc atccccttct ggctgggaag
    13981 cgctccaacg gatcggcgaa tccttacgct tgcagtggga tcgtgtattc atccgataca
    14041 gtgcgcgcga tcaactggcg gtcgtctaca gtcttcggga tagcagcgat tctgcccgcg
    14101 actacctgag ccgatggatg attacgctca agacaacggt ctcccaaaca ctgggacaaa
    14161 ttaaagcgca cgccaagtcc gcaagcccct tagccctgtg gctcgcggca atccttccgg
    14221 gcgtcggtct ggcactgctc ttcgtggtga tcaagaaaac atggtgggac accattccct
    14281 ctcaccgccc cacgatacgc aagcaagagt acatcgccca gctctataaa aagatgctcg
    14341 atgtcgcagc taaacagggc atcatcaaac acccgacgtt gacgccgatg gaatttgtac
    14401 agctagtccg tcgcgaatgg tctgaagccg ggtcaatggt ggcagatttg acgatgctct
    14461 attgccgagg ccggttcagt ggatccccgc tcacccacga ggaactctcc ctcgcaggtg
    14521 aaaagatccg cgcgctccaa cggatgagcc gcgctacggg ataatcgcca agaaccgaca
    14581 caatcaggcc aaggcgtcca gattctgccg gagcggagcg agatcgacgt tacctccggt
    14641 cagcacgacc gctacccgtt tcccccgcag ttgcggctct cttctcaaca acggagcgag
    14701 tgcgaccgca ctagatggtt caataacgag cttcagctgc tcctgcgcaa agcgcatggc
    14761 ctgtagaatt tccgtctcct ctacgatgaa gaaccccgcc agataccgcc tgagaacggg
    14821 taatgtccta tctcccagac ttgttctgag gccatcggcg atggtgttgg gattggacat
    14881 cggcacaaca cgattctgcc tgaccgactc gattgcatcc aatgcaccag ccggctcgca
    14941 ggcaaagatc tttatctgcg gccgacaggc ccctgcggca atgcacaacc ccgagagtaa
    15001 tccgccgccg ccgaccggag ccaggacgat atcgaggtca ttcacttgat caagtagttc
    15061 aaccatgatc gtcccttgcc ccgcgatcac caacggatcg ttaaatgggt ggacgagcat
    15121 cgcgccggtg tctttcatca tctctcgcaa catcgcttca gcgcgagtac ggtcttccgc
    15181 cacgcaaacg gcagcgccat agtcgagtgc ggcttgccgc ttcatcgacg agtgcgggtt
    15241 cggcatcacg acgtgggcct tcgctcccag aatccggcac gcgagggcaa ggccctgcgc
    15301 atggttccct gaggaaatgg tcgcaaacgt gctggtgtga tggctggcca tgtgtcgcgc
    15361 gatcgcatga taggccccgc gaaacttgaa tgcaccgacg cgctgaaagt tttcacactt
    15421 gaagaacacg ctggcggccg cgcgctcgtc aagggcccga ttggacttca cgggggtgac
    15481 gagcgctaca ccccgcaaca cgtcgactgc ctgttcaatc agcgaatacg tgacatcgtt
    15541 cacgaggcca tccagagata taaggaaacc tgaacaattg agagcaccgt ctctgccctg
    15601 gctatcagaa aaacacagga gagaaatgta aagggattgg agcgggaaaa gggatttgaa
    15661 ccctcgaccc tcgccttggc aaggcgatgc tctaccactg agctattccc gctcgaaaag
    15721 gtgacctgaa gaggggtgga ttttactgag ggttcggtag cctgtcaagc aaccttacgg
    15781 gccatcgatt tcttgttacc ttcgttacaa cgtgccatga acgctacgaa aaggtaacgc
    15841 ttccattttc agcataaggt attgatgtaa ttatgttttg gtcatcttct tgtcttcctg
    15901 gtttgcctgc cgatacatca tgtacccatg agtgctgcat ttgacgcctt ttgagaagcg
    15961 agaaaatcgt tattttacct tgtaatttca atgtattaga ttatgtgact ccagcgcggc
    16021 aaaagccgcg actatggtgt gtatcttgcc aatgaagtga ccccatgatg cttggaaact
    16081 atggaaaaat gaccaaggca atcacggaag caacgacttc cgcatgggac attgtccaag
    16141 aatctgagca gctctttctc aacagaaggg gggtgggaag cgttctggtt ttcagtcagt
    16201 gagggacagt gggctcgttg agccctcaac ttaggaggtg tcaccaatgt ttgtgtcacg
    16261 cagacagttt ttaaaagtct ccgcggggac ggttgccgct gtggcggtgg ccgacaaagt
    16321 cctggcgttg accgcgctgc agccggtcat cgaggtcggg aacccgttgg gcgactatcc
    16381 ggatcgatcc tgggaacggg tctaccacga ccagtaccgg tacgattcgt cgtttacctg
    16441 gtgttgttca ccgaacgaca cgcacgcctg ccgcatccgg
//
LOCUS       DATIFR010000116         8544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1034178, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000116 DATIFR010000000
VERSION     DATIFR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034178"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VLD60_06685"
     CDS             complement(<1..749)
                     /locus_tag="VLD60_06685"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSB44677.1"
                     /translation="MMMRWPAGVVAFVWMVLGAWVSPSHAAADREKLLSEPGVYGTFA
                     AFTLDDEWAKEDQATRIAHVTVLRGVVEQHREKVAIDLYLMRGLSDHADLMFRVHAAE
                     LRDTQKFLVDLQNSLFGKHLKTAGIMNGLTKKANYVPGFPDQMKADLKTASEPGPKPY
                     AIVIPIRKNADWWGLDQDKRAAMMKEHTEAALPYHKTVKRKLYHSTGLDDLDFITYFE
                     TSKMEDFHSLILSLEKVKEFQYTRRFGHPTL"
     gene            complement(746..2083)
                     /gene="purB"
                     /locus_tag="VLD60_06690"
     CDS             complement(746..2083)
                     /gene="purB"
                     /locus_tag="VLD60_06690"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSB44678.1"
                     /translation="MIERYTRPKMKAIWDLKRKYEIWLEVELQACAAFERVGQAPRGT
                     AAKIRKKAKINVDRIAEIEKVTKHDVIAFLESLMDTVGPEHRFLHMGLTSSDIVDTSL
                     AVQMTEALDLILGGVEAFLEVLKRQAFRYKNQVMVGRSHGIHGEPISFGLKMANWYEE
                     VSRHLERLRQVRSEIAVGKLSGAMGTFAHQGPDIEEYVCTKLGLTFDPVSNQVVQRDR
                     HAAYATALALLAATIEKIATEVRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPIVSEN
                     LCGLARLVRANSLAAMENVALWHERDISHSSVERVVMPDSTILVDYMLSKVTDLIKHL
                     VVYPENMKRNLELTGGLVYSQRLLLALVEKGAQRKESYEAIQRNAMASWKGAGGLQKL
                     VEQDPFIGKHLKKSEIAACFNPKYYLRHLDQIFRRVFQSRGQRTSNRRTKGAR"
     gene            complement(2114..2572)
                     /gene="nusB"
                     /locus_tag="VLD60_06695"
     CDS             complement(2114..2572)
                     /gene="nusB"
                     /locus_tag="VLD60_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSB44679.1"
                     /translation="MGSRHQSRERALQILFQYDIHGKPGLWLEEFWKPLKDSEESRAF
                     AERLVAGVREHQKELDALIGRYATNWKVSRMQIVDRNILRMGTYELLWLDEVPAKVTM
                     NEAIELAKDFGDEEAAKFVNGILDKVLATEPKLEAKRHEASGVKGAGRKG"
     gene            complement(2788..3333)
                     /gene="ribH"
                     /locus_tag="VLD60_06700"
     CDS             complement(2788..3333)
                     /gene="ribH"
                     /locus_tag="VLD60_06700"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSB44680.1"
                     /translation="MKLRKGSHNATGLKFGIVVAKFNKFVTSKLLTSCIEALTKHGVA
                     AEDIEAVRVPGAFEIPLVARTLAATKRFDAVICLGAVIRGDTPHFDYISTEASRGIGQ
                     AALDADVPIIFGVLTTDTVAQAIERADPGKFNRGGEAAKSAIEMATVMRMIRGGDGAA
                     PASEAVRGNGAKARPAQRRAK"
     gene            complement(3478..4686)
                     /locus_tag="VLD60_06705"
     CDS             complement(3478..4686)
                     /locus_tag="VLD60_06705"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSB44681.1"
                     /translation="MSSPFNSIEDAIRDIKKGKFVILVDDEDRENEGDLVIAAEKVTP
                     HAINFMAKHARGLICLALTPERVEELQLPQQAVENTATFGTAFTVSIDARRDITTGIS
                     AADRATTIHVAIDPKSKPSDLARPGHIFPLKAQKGGVLRRAGQTEGSVDLARLAGLQP
                     AGVICEIMNEDGTMARVPELAKFAKAHKLKLVTVKALIEHRMRRETFVKRMATAQLPT
                     TFGEFEAVAFENEVDNVTHIALVKGKVDDGTPTLVRVHSGCLTADALGSLRCDCRDQL
                     HKAMEIIQKEGRGVLLYLNQEGRGIGLLNKIRAYGLQDQGSDTVEANLMLGFKADLRD
                     YGVGAQILANLGLHQIKLITNNPRKIVGIEGYGLNVVERVPIEIQPRAANMKYLRTKK
                     HKLGHILKKV"
     gene            complement(4783..6009)
                     /locus_tag="VLD60_06710"
     CDS             complement(4783..6009)
                     /locus_tag="VLD60_06710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44682.1"
                     /translation="MKAIFSALRSGHWPSLIGAWLHFEVSFMAWLLIGALGVSIAEDF
                     GLSSTQKGLLVACPLLGGAFLRVGVGLASDRIGAKTTGLYLLAGSMGAMVWGWLAATS
                     YLQLLGVGLLLGIAGASFAVALPVASRAYPPAHQGLALGVAASANSGTVLAMFFAPRL
                     AQLVGWHGVFGLMMAPLTLTFLLFWRLVQSDAGQSSTERNPDWWRDLVDLMRLPSVYW
                     LCLLYGVTFGGFVGFCSVMPIFLHDQYGLNIVEAGSLTALCGLLGSLIRPVGGFAADR
                     LGGMVLLGPVLAAIGGIMMGLGLLPAIGWAVVLLIGGISTMGFGNGVVFQVVSERLPK
                     QIGIASGVIGAAGGFGGFLLPLFLGLLKDLSGTFRTGLWLFAAVSGAAALSVAVLIGR
                     AYRRNREGSEPDRFPR"
     gene            6173..7156
                     /locus_tag="VLD60_06715"
     CDS             6173..7156
                     /locus_tag="VLD60_06715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HSB44683.1"
                     /translation="MGKKDDDLDQAPLEPEVLGPSEEEPATELSSSSEPGSQSSPDTT
                     AVVPVTALQHYLAEIRRHPYLTKEEELLLFEEYRTHGSRDAAVKLIMANLRVSVAIAS
                     EYLHTGADHMDLIQEGNVGLMQAIKKFDPAKNVRFYAYAAWWSRAYILRYLLNTYRLV
                     KIGTTQDQRKLFYNLKKEKAKLEREGFAPDTKLLADRLNVRERDIVEMDQRLGSWELS
                     LDQPIGEDQQGSLLDVLPSQQVPADEQLAQDELKTLFRTKLAEFIKTLDERDEDILRN
                     RILSETPLTLDDLGAKYGITKERTRQLEARIIKRLRDYIKKDVKDFDRLRI"
     gene            7432..7731
                     /locus_tag="VLD60_06720"
     CDS             7432..7731
                     /locus_tag="VLD60_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSB44684.1"
                     /translation="MSTAAKEKKNIAKDFQPLGDRVFVTYTEELERTAGGIYVPDSAK
                     EKPQRGIVQAVGKKVENVKVGDQVLFDKYSGSKLRIEDEECLILKEEDILGIFTS"
     gene            7772..>8544
                     /gene="groEL"
                     /locus_tag="VLD60_06725"
     CDS             7772..>8544
                     /gene="groEL"
                     /locus_tag="VLD60_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSB44685.1"
                     /translation="MAKQLLYSEAARASILKGVNQLADAVKATLGPKGRNAILDKKFG
                     APTITKDGVTVAKEIELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIFREG
                     VKNITAGANPMEIQRGINKAVEVVIAELKKLSKPCQNKTEISQVGTISANNDKTIGDL
                     IAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMECSVEEPL
                     ILINEKKVSSMKDLLPILEQVAKMGKPLVIIAEEVEGEAL"
BASE COUNT         1871 a         2694 c         2244 g         1735 t
ORIGIN      
        1 atcaacgtcg gatggccgaa tcgccgagtg tactgaaatt ctttcacctt ctccaacgac
       61 agaatgaggc tatggaaatc ctccatcttg gaggtttcaa aatacgtgat gaagtccagg
      121 tcatcaagtc cggtggaatg atacagtttg cgcttcaccg tcttgtgata aggcaacgcg
      181 gcttccgtat gctctttcat catcgcggcc cgtttgtcct gatcgagccc ccaccagtcg
      241 gcgttcttcc ggataggaat cacgatcgca tagggcttcg ggcccggttc gctggccgtc
      301 ttgagatcgg ccttcatctg atcgggaaac cccggcacat agttggcctt cttggtcagt
      361 ccgttcatga tgccggccgt cttgagatgc ttgccaaaga ggctgttttg gagatccacg
      421 agaaactttt gggtatcccg cagttcggcc gcatgcaccc ggaacatcag atcggcatgg
      481 tcggagagcc cacgcatgag atagagatcg atcgcgacct tctcccgatg ctgctcgacg
      541 acgcctctca acaccgtcac atgtgcgatg cgcgtggcct gatcctcctt cgcccattcg
      601 tcatcgagcg tgaaagccgc gaaggtgccg tagacccccg gttcgctcag gagtttttcc
      661 cgatccgccg ctgcatgaga cggcgacacc caagccccca gcaccatcca tacgaatgcg
      721 actacaccgg ccggccatcg catcatcatc tggctccttt cgtccgtctg ttggaagtcc
      781 gttgcccgcg ggactgaaag actctgcgaa agatctggtc gaggtgtcga agataatatt
      841 tcgggttgaa gcatgctgcg atctcgctct tcttcagatg cttgccgatg aaggggtctt
      901 gctccaccaa cttttgtaaa ccgcccgccc ccttccacga cgccatggca ttgcgctgga
      961 tcgcttcgta tgattccttc cgctgcgcgc ccttttccac gagcgccagc aacaagcgct
     1021 gcgaatagac gagcccgccc gtcagttcaa ggttccgttt catattctcc ggatagacca
     1081 cgagatgctt gatgaggtcg gtcaccttcg acagcatgta atccacgagg atcgtgctgt
     1141 ccggcatgac gacgcgttca acggaggaat ggctgatgtc gcgctcatgc cacagcgcca
     1201 cgttctccat cgctgcgagg ctgttggccc gcaccaagcg cgccaatccg caaagattct
     1261 ccgacacgat cggattccgc ttgtgcggca tcgcggagga acctttctgt ccttccgaaa
     1321 aatactcttc agcctcgagc acctcggtcc gctggagatg ccgcacctcc gtggcaatct
     1381 tttcaatcgt ggccgcaagt aacgcgagcg ccgtcgcata ggccgcatgc cggtcgcgct
     1441 gcacgacttg attggaaacc ggatcgaacg tcaggccaag cttggtgcag acatactctt
     1501 cgatatccgg tccctgatgg gcaaacgtac ccattgcacc ggacagtttg ccgacggcga
     1561 tctcactccg cacctggcgt agccgctcca aatggcggct gacttcctcg taccaattcg
     1621 ccatcttgag cccgaaggaa atcggttcac catggatgcc gtgcgaacgg cccaccatca
     1681 cctgattctt gtaccggaac gcctgccgct tcaatacctc aagaaaggct tcgactcccc
     1741 caagaatcag atccaacgcc tcggtcattt gcacagcgag cgacgtatcg acgatgtctg
     1801 acgacgtcag gcccatgtgc agaaaccggt gctcaggccc cactgtgtcc atgagcgatt
     1861 cgaggaacgc gatcacatcg tgcttcgtga ctttctcgat ttcggcaatc cgatccacat
     1921 tgatctttgc cttcttgcgg atctttgccg cggttccgcg cggagcctgg ccgacccgct
     1981 cgaaggccgc gcaggcttgc agctcgactt cgagccagat ctcatactta cgcttgagat
     2041 cccagatggc cttcatcttc ggtctggtat accgttcgat catctgttca tctcgaccct
     2101 tacccttaac gccttacccc ttacgtccgg cgcccttcac ccctgacgcc tcatgcctct
     2161 tggcttccag cttcggctcc gtcgccaata ctttatccaa gattccgttc acaaatttgg
     2221 cagcctcctc atccccgaaa tctttcgcga gctcgatcgc ttcgttcatt gtcaccttgg
     2281 ccggcacctc gtcgagccag aggagctcat aggtccccat gcgcagaatg ttccggtcga
     2341 cgatctgcat gcggctcacc ttccagtttg tcgcatagcg gccgatcagc gcatccaact
     2401 ctttctgatg ctcgcgcaca cccgcgacca accgctcggc aaacgccctc gactcttcgc
     2461 tatccttgag cggtttccaa aactcttcga gccagaggcc cggcttcccg tggatgtcgt
     2521 actgaaacaa gatctgcagc gcccgctcgc gggactggtg gcgagaaccc atgttagccc
     2581 acattaccca tgaatgtttc gacggcagcc gccgatacgc cgcttagaca agctggcatc
     2641 gaacttcgcg gcatgccagc gggcgggctc gcccctcccg acctcaacgt actgaaataa
     2701 gtacgcttcg gcctctcggg gctccacgcg cccgtctcac ctggcatctt ggcggtttcg
     2761 tgaccaacca tcatgagtat tgtgggccta cttggcacgc ctttgcgccg gcctcgcctt
     2821 cgcaccgttg cctctcaccg cctctgacgc aggagccgct ccgtctcccc cgcgaatcat
     2881 ccgcattacc gtcgccattt cgatcgccga cttcgccgcc tcaccgcccc gattgaactt
     2941 gcccggatca gcccgctcga tcgcttgcgc cactgtgtcg gtcgtcagca cgccgaagat
     3001 gatcggcaca tccgcatcca gcgccgcctg tccaataccg cgactggctt cagtactgat
     3061 gtagtcaaag tgcggcgtgt ccccgcgaat gacggcaccc aggcaaatca ccgcgtcgaa
     3121 tcgctttgtc gcggccaacg tgcgcgccac cagcggaatc tcaaaggccc ccggcacccg
     3181 caccgcctcg atatcctccg cggcgacacc atgctttgtc agcgcctcga tgcaggaggt
     3241 caacaacttg ctcgtgacga acttattaaa ctttgcgacg acgatcccga acttcaatcc
     3301 cgtcgcattg tgcgaacctt tacgcagctt catagaatcc tttgtgaagc ctcggctgct
     3361 caaaatggcc tcccggcgag gccgcaggcg agaaacggcc ggaggcgtac tctctggagt
     3421 acgttgaggt tcgattcgag ccgagaacga agctgggggg tcatttttca gcagccgcta
     3481 caccttcttg agaatgtgcc ccaacttgtg tttcttcgtc cgcaagtact tcatattcgc
     3541 cgcacgcggc tgaatttcga tcggcacccg ctcgacaacg ttcaacccgt agccttcgat
     3601 tccgacaatc ttccggggat tgttcgtgat caacttgatt tgatgcaggc caagattcgc
     3661 cagaatctgc gccccgacac cgtaatcgcg caggtcggct ttgaatccga gcatcagatt
     3721 cgcttcaacc gtatcgctgc cttggtcttg taacccgtag gccctgatct tgttgagcag
     3781 tccgatcccg cgcccttctt gattgagata gagcaggacg ccacgccctt ctttttggat
     3841 aatctccatc gctttgtgca attgatcgcg acagtcgcac cgcagcgaac ccaaggcatc
     3901 ggcggtcagg catccggagt ggacccgcac cagcgtcggg gtgccgtcgt ccaccttccc
     3961 cttcacgagc gcgatatggg tcacgttatc gacttcgttc tcaaacgcca cggcttcgaa
     4021 ctcgccgaag gtcgtcggga gttgtgcggt cgccatgcgc ttcacgaatg tttcccggcg
     4081 catgcggtgt tcgatcaacg ccttcaccgt cacgagcttg agcttgtggg cttttgcaaa
     4141 cttggccagt tccggcaccc gggccatcgt gccgtcttcg ttcatgatct cgcagatcac
     4201 accggccggc tgcagaccgg ccaatcgcgc cagatcgacc gacccttccg tctgtcccgc
     4261 acgacgcagc acgccgccct tctgggcctt taacggaaag atgtgaccgg ggcgcgcgag
     4321 gtcactgggt ttggatttcg gatcgattgc gacatgaatc gtcgtggcac ggtcggccgc
     4381 agaaatgccg gtcgtaatat ccctgcgcgc atcgatggac accgtgaagg ccgttccaaa
     4441 agttgcggtg ttctcgaccg cttgctgcgg caattgcagt tcttcgactc gctccggcgt
     4501 gagggccagg cagatcaagc cacgggcatg ctttgccatg aaattgatgg catgaggggt
     4561 gaccttctcg gcagcaatga cgagatcgcc ttcattttcc cggtcttcgt catccaccag
     4621 gattacgaac ttccccttct taatgtctcg aatcgcgtct tcgatgctgt tgaatgggct
     4681 ggacataatt ttactgttgg ctgttctacg gtgaccgttc aaggaacaac caatttatca
     4741 tttaacattg aacatgtaac attgtttttt tgcccctgga cgtcaccggg gaaaacggtc
     4801 tggttcggaa ccttcgcggt ttcttctgta tgctcgtcca atgagcacgg ccacactgag
     4861 ggctgctgcc ccggatacgg ctgcaaacaa ccacagccct gtgcggaacg tcccggacag
     4921 gtctttgagc aggccgagaa acagcggaag aaggaagccg ccgaatccac cggcggcgcc
     4981 gatcacgccg gacgcaatcc cgatctgttt gggaagccgt tcagacacca cctggaagac
     5041 aacgccgttt ccaaacccca tcgtgctgat cccgccaatg agcagcacca cggcccagcc
     5101 gatcgccggc aagagtccca agcccatcat gatccctccg atagcagcca agaccggccc
     5161 taacaggacc atgcctccta gccggtcggc ggcgaacccg ccaaccggac ggatcaggct
     5221 tccgagcaac ccgcataagg ccgtcaaaga gcctgcctca acgatattta gtccatactg
     5281 atcgtgcaga aaaatcggca tcacgctgca aaatccgaca aaacccccga atgtgactcc
     5341 ataaagaaga cacagccaat acacggaggg cagccgcatt aaatcgacga ggtctcgcca
     5401 ccagtcggga ttccgttccg tactcgactg gcccgcatcc gattggacca gcctccagaa
     5461 gagaaggaaa gtgagcgtca gcggcgccat catcagaccg aacacgccat gccacccgac
     5521 gagctgagcc aatcgtggag caaaaaacat tgcgagcacg gtcccgctat tggccgaggc
     5581 tgcaacacct aacgctagcc cctgatgggc cggcggataa gcccggctcg caacaggcag
     5641 cgcaacagca aaactggcgc ccgcgatacc cagcagtaac cccactccaa gcaattgcag
     5701 atagctcgtc gcggccaacc atccccaaac catggctccc attgagccag ccaataaata
     5761 caggcccgtc gtcttggccc cgattcgatc gctcgccaat ccgactccga ctcgcaaaaa
     5821 cgctccgccg agcagcgggc aggcaaccag cagccccttt tgcgtggagc tcaacccgaa
     5881 atcttccgca atcgacacac cgagggcgcc aatcaacagc cacgccatga aactgacttc
     5941 gaagtgcagc cacgcgccga tcagcgaagg ccaatggccg ctccgcaatg ccgagaatat
     6001 cgctttcatc gcatcatccg actctctgaa ggaaatgagc ccatcccctg tccgcagcta
     6061 gacaacacgc cactataggg gacggccttt cgaacctgca agacggcgtg gagcgcctcg
     6121 cgcgagagac ggtttgtccc gaaacgtggt tctgttatag tcagcggctt gtatgggaaa
     6181 aaaggacgac gatctcgacc aagcccccct cgaacctgaa gtgttgggtc cttcggagga
     6241 ggaaccggcc acggaactga gttcctcctc ggagccaggc tcgcaaagtt cacccgatac
     6301 caccgcggtg gtgccggtta cggccctcca gcactatctt gccgaaatcc gccgccatcc
     6361 gtacctcaca aaagaagaag agctcctgct gttcgaggaa taccggacgc acggcagtcg
     6421 cgatgcggcc gtcaagctga tcatggccaa cctgcgcgtg tcggtcgcca ttgcctccga
     6481 gtacctccac acaggcgccg atcacatgga cttgatccag gaaggcaacg tcggcctcat
     6541 gcaggcgatc aagaagttcg acccggcgaa gaatgtccgg ttctatgcct atgcagcctg
     6601 gtggtcgcgc gcctatatcc tgcgctacct cttgaacacc taccggctgg tcaagatcgg
     6661 cacgacgcag gaccagcgga agctcttcta caatttgaaa aaggaaaagg caaagctcga
     6721 acgtgaagga tttgcccccg ataccaaact cttggcagat cgtttgaacg tccgggaacg
     6781 cgatatcgtt gagatggacc agcggctcgg cagttgggaa ctgtctctgg atcagccgat
     6841 cggcgaggac caacaaggca gcctgctcga cgtccttccc tcccagcaag tcccggcgga
     6901 cgagcaactg gcgcaggacg agctcaagac actgttccgc accaagctgg ccgagttcat
     6961 caagaccctg gatgagcggg acgaggacat tttgcgcaac cgcattctct cagaaacacc
     7021 actgacgctg gatgacctcg gcgccaagta cggcattaca aaggaacgca cccgccaact
     7081 cgaggcccga atcatcaaac gcctgcggga ctacattaag aaagacgtga aagatttcga
     7141 ccgcttacgg atctgagtgc acgcaaccta tgtttttcat ccgcccctga atttaggtgt
     7201 ggcatatcga attctcaata ggttatgata gcgattgtct ggagacggtg ggtgctggag
     7261 aaaaatctgt ttcccccctc ttgactcgga ctaacccgag gctatctccc cactcgaact
     7321 ttgtgactgc cggttgcgct cgttcggggt atcagacgta cgccttcgag ttcgaaccag
     7381 cttcaaatcg ttgatctatc gagttaactt tcatcacgag gaggttctgt tatgagcaca
     7441 gcagccaagg agaagaagaa catcgccaag gatttccagc ccctgggtga tcgggtgttc
     7501 gtcacctaca ccgaggaatt ggaacggacc gcgggcggga tttacgtgcc ggattcggcg
     7561 aaggaaaagc cgcaacgagg catcgtgcag gccgtcggca agaaggtcga gaacgtgaag
     7621 gtcggcgacc aagtcctctt tgacaagtat tcaggcagca agctccggat cgaggatgaa
     7681 gagtgcttga tcctcaagga agaagacatc ctcggcatct tcaccagcta attgatcaac
     7741 aagacgacga ctgaactaac ggaggaacta tatggctaag caattactgt acagcgaggc
     7801 ggcacgtgcc tcgattctca agggcgtgaa ccagctggcg gatgccgtca aggcgacgct
     7861 cggccccaaa ggccgcaatg cgattctcga caagaaattc ggtgctccga ccattaccaa
     7921 ggacggcgtg accgtagcca aggaaatcga actcaagaac ccgtatgaga acatgggcgc
     7981 ccagctcgtc cgcgaagtcg ccagcaagac cagcgacacg gcgggtgacg gcaccaccac
     8041 cgccaccgtg ttggcccagg ccatcttccg cgaaggcgtg aagaacatca ccgccggcgc
     8101 gaacccgatg gaaattcagc gcggcatcaa caaggccgtt gaagtcgtca tcgcggagct
     8161 caagaagctc agcaagccct gtcagaacaa gaccgagatc tcccaggtcg gcaccatttc
     8221 agccaacaac gacaagacca tcggcgacct catcgcagaa gcgatggaga aggtcggcaa
     8281 ggatggcgtg atcacggtcg aagaagccaa gtcgatgacc acctcgctgg acgtcgtcga
     8341 gggcatgcag ttcgatcgcg gctacatctc cccctacttc gtcaccaatg ccgagcggat
     8401 ggaatgttcc gttgaagagc cgctcatcct gatcaacgaa aagaaagtca gcagcatgaa
     8461 ggacctcctc ccgatcctcg agcaggtggc caagatgggc aagccgctcg tgatcatcgc
     8521 ggaagaagtc gaaggcgaag cgct
//
LOCUS       DATIFR010000117         8428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1800352, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000117 DATIFR010000000
VERSION     DATIFR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800352"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VLD60_06730"
     CDS             <1..758
                     /locus_tag="VLD60_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44686.1"
                     /translation="LPPASNRTDMYDKIGRVLKLPPGQLAKLADLQRREQLKKRLGDQ
                     PNPLLHDVRELILRKCHPGKNRQIRTIFEKQPFGELERFVTQKLLGVAKGVARQELEN
                     PAWLRRVARLSKKSYPEMRVTVLKFLDATVFDLSDEYCIAFLDPLIESWDIDLATFCI
                     EIVLNRRVSPGHHKTFEFIERGPELPSEEEPGLTEFLQDSSLSGDATETELEFLRTLR
                     FEDKRPTSIYYYRELQNLRDPLHFRAPRGGRKA"
     gene            755..1183
                     /locus_tag="VLD60_06735"
     CDS             755..1183
                     /locus_tag="VLD60_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSB44687.1"
                     /translation="MSGTEKVTWQAYPSWNQFAWLFMVSLAAGSRGLQLFRLGLDGWG
                     IWLSGAAALLVCAAALRRWALYLLTSTRVVIRNGYTGTDIQTIALDDIREITLSQGPV
                     AQFLNIGTLMIHPKSESRPLLLQGVHDPEVIKTRLEAHRP"
     gene            complement(1482..2381)
                     /locus_tag="VLD60_06740"
     CDS             complement(1482..2381)
                     /locus_tag="VLD60_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSB44688.1"
                     /translation="MEFVPWYEKPKRGRRPWHVCGADLCLPITLAGVLLSASEALSGA
                     ITIAGNGPELRMVEQLTRAFEKKYPGAVAEIRWDSSFHPISMVKSGEADFAVAGESDA
                     ELVAIPIAWDGIAVVVDFTNPLREITMQQLAAMFSGVITRWSALGGADNRIELIDRPP
                     NQHIRGTFEAILGIAGKIPATAVVKRSDQRAISSVAGNGSAVTYASMGVALDAVTYGV
                     GVALLNIEGVEPAKETVKDGRYRLRRPVLLLRKHETNPTAEAFSEFARSKEGQDIVEE
                     MFAPYTNPQATPHAVRREKLERP"
     gene            complement(3073..3366)
                     /locus_tag="VLD60_06745"
     CDS             complement(3073..3366)
                     /locus_tag="VLD60_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44689.1"
                     /translation="MRIHLTMQRLVLLSCVLAISAWATPPTYAADPLVEGTMVVAPIG
                     AMDVVASGSVEDTLKACLARIPEIASVGQRMLAEQNCAGEEGTRQLTPVAPKF"
     gene            3863..4048
                     /locus_tag="VLD60_06750"
     CDS             3863..4048
                     /locus_tag="VLD60_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44690.1"
                     /translation="MWTGNMTGSEQLVRTLLALAFIGFSSQEHTTLIQAMAGYVVGGI
                     LLTSALFAQCATRKLLE"
     gene            4356..4703
                     /locus_tag="VLD60_06755"
     CDS             4356..4703
                     /locus_tag="VLD60_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44691.1"
                     /translation="MSNPIPNHMKTASMWVPIPDGTRVKLRNETLEGVIDGLTEFVSG
                     PDRNPDGKTQYRMNIGIPTRQLVAEDNLSILLGGDDLVIMVRQKEPYRRSVTEQLRGK
                     FAADRFKKSAKSH"
     gene            4991..5170
                     /locus_tag="VLD60_06760"
     CDS             4991..5170
                     /locus_tag="VLD60_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44692.1"
                     /translation="MSKTTLRKRQRQQTQQERQQEKAVRREQRNLEKSVLLRSAGQED
                     PDLNGMVAGPQRLRD"
     gene            5445..5771
                     /locus_tag="VLD60_06765"
     CDS             5445..5771
                     /locus_tag="VLD60_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB44693.1"
                     /translation="MGSKLYVGGLPFAATEAQLTTLFAAHGTVESARVIADKFTGQSR
                     GFGFVEMSTPEEAKAAITALNGSQMDGRSLTVNEAKPQEPRSGGGGGRFSGGRGGNDF
                     GSRSRF"
     gene            5842..6705
                     /locus_tag="VLD60_06770"
     CDS             5842..6705
                     /locus_tag="VLD60_06770"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HSB44694.1"
                     /translation="MRDKTISSQDIRRAHPNYMTTILFSLVAGGALIGVPVFGLAYGY
                     TWLDWTMFGVLYVMTGLGITVGYHRLMAHRSFDCPGWVKAGFLVAGGWALENSALKWA
                     ADHIRHHASCDQEADPYNAKRGFWYSHCGWLFFHDVHANEKYASRLRQDPIVMWQHRY
                     YLPIVLSGLGLTFVVGFLHNGWIGGLGCFLLAGVGRTFAVLNSTFCINSICHIWGRQP
                     HGQADSSRDSWLVSLVTFGEGYHNYHHTFQCDYRNGPRWYNFDPSKWLIFALSKLKMA
                     SSLRIASSTHA"
     gene            6791..7528
                     /locus_tag="VLD60_06775"
     CDS             6791..7528
                     /locus_tag="VLD60_06775"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSB44695.1"
                     /translation="MYAANLRSPDQASDPNDSAYSFFFATQNAVQKNTKRLERLPNHG
                     RMWVMERQPHMSRTQRFFLFSLLASLALATLVCTPSSAEYPTLISIGPRVGFSQKAPL
                     LGQQQKYYFHMTDVAAVFRLPWSWPLGETSWNLETRLITSAGLLQATGDSALMVTIVP
                     DLALSAWEGLITFDAGAGAGFFSNYKFGEQDFGGPVQIVATLGVTVNPFSHAYAGFRA
                     QHFSDAGVYGPSSLGVDMYIVELGYRF"
     gene            complement(7607..>8428)
                     /locus_tag="VLD60_06780"
     CDS             complement(7607..>8428)
                     /locus_tag="VLD60_06780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB44696.1"
                     /translation="SPTQRKQAVTTRWALPQHDYWSTPFAEALLQRLELFPGATILDV
                     ASGHGIPAFHVAERVGAAGQVLGIDLSEGQVASARAVQGAQLPWLRFECLDMRALPPE
                     LPLFDRITGNLSVMFMRPDRLDSIRGLIQHLQPGGQIVLTFPSLGTFDSLWRRIDQEM
                     AVRGLLTERRRLEAYIAERPSAEEGRRWLYDTGLERVVVAEYPLEVATGSGAAFLYHP
                     LLRGGFLDDVFDCFDDQRLANEVMTRVSEDIDSFVPLIAQRCVLSGWRKKMGETV"
BASE COUNT         1899 a         2519 c         2243 g         1767 t
ORIGIN      
        1 aacttcctcc ggcgtcgaat cgaaccgata tgtacgacaa gatcggacgg gtcctgaaat
       61 tgccaccggg acaactcgcc aagctcgccg atctgcagcg ccgcgagcag ctgaagaagc
      121 ggctaggaga tcagccgaat ccgttgctgc acgacgtgcg ggagttgatt cttcgaaaat
      181 gccatcccgg gaaaaaccgg caaatccgca ccatcttcga gaaacagccg ttcggggagc
      241 tcgaacggtt cgtgacgcag aaactgctcg gcgtcgcgaa gggcgtcgcc cggcaggaac
      301 tcgagaatcc tgcgtggctg cgacgggtcg cccgactcag caagaagagc tatccggaga
      361 tgcgggtcac cgtgctgaag tttttggatg cgacggtctt tgatctgtcg gacgagtact
      421 gcattgcgtt cctggaccct ttgattgaat cctgggacat tgacctcgcc accttttgca
      481 ttgagattgt cctcaaccgc cgggtatcgc cagggcatca taaaacattc gagtttatcg
      541 aaagaggacc ggagctgccc tcggaggaag agcccgggct cacggaattt cttcaagatt
      601 catcactcag cggggatgcg acagagacgg aactcgaatt tctgcgcaca ctcagattcg
      661 aagacaaacg accgacgtcg atctattatt atcgcgaact gcaaaacctc agagaccccc
      721 tccactttcg ggccccacgc ggagggcgca aggcgtgagc ggaacagaga aggtaacctg
      781 gcaggcctat ccttcttgga accaattcgc ctggctgttc atggtcagtc tagccgccgg
      841 ctctcgcggt ctacaattat ttcgacttgg cttagacgga tggggaatat ggctgagcgg
      901 tgccgcggcc ctgctggtat gcgcggccgc tctacgccga tgggccctgt accttctgac
      961 gtccactcgc gtggtcataa gaaacggcta cacgggtaca gacattcaga cgatcgcgct
     1021 ggatgacatc cgcgagatca cgctctcaca aggccccgtt gcacaattcc tcaacatcgg
     1081 cacgcttatg atccacccga agagtgagag ccgtcctctc ttgctgcaag gagtacatga
     1141 tcccgaggtg atcaagactc gcctggaggc acacagacct tgacggcgat agcttcttct
     1201 cctgaatcac tctaacactc ctccctcttg tggcctggcc aagggcccat gagatcggcc
     1261 tcgacgatct tttctccaac cacttctctt atcgagtcag atagaacgtc aggatcagcg
     1321 ctccgaccat ggcaatcagg gcaagcagta ctgtgagctc cggccacata tcgcgcaaga
     1381 aacgcatagg tgcctcctgc cctgaatgtt tacaccgtta gaacttgaaa gaacgaccgc
     1441 gttcgcacgg ggatttcagt ccatttctct cggaactgcg atcacggtct ctccaatttt
     1501 tctcggcgca ccgcgtgagg cgtagcctga ggattggtat agggggcgaa catctcttcg
     1561 acgatgtctt gcccctcctt cgaacgagca aattctgaga aagcctccgc tgtcggattg
     1621 gtttcgtgtt ttcgaagcag cagcacgggc cggcgcaacc gataccgccc atctttgacg
     1681 gtctccttgg ccggctcgac tccctcaatg ttcaagagcg ccacgccgac accatatgtc
     1741 acggcatcca gcgcgacacc catcgacgca taggtcactg ccgacccatt gcctgccacg
     1801 ctgctgatgg ctcgctgatc cgaccgcttc acgacggcgg tggcaggaat cttcccggca
     1861 atccccaaga tcgcttcaaa cgtgccacga atatgttggt tggggggacg gtcgatgagc
     1921 tcgattcggt tatcggcgcc acccaaagcc gaccaccgag tgatcacccc cgaaaacatg
     1981 gcggcgagtt gctgcatggt gatctcccgt agtggattgg taaaatccac cacgacggcg
     2041 ataccgtccc aggcaatggg aatggccacc agttcggcat cactctcgcc cgccacggca
     2101 aaatccgcct cacccgattt caccatgctg atcgggtgga acgacgaatc ccatcgaatc
     2161 tcagcgacgg cgcccggata cttcttttca aatgctcgag tcagttgctc gaccatgcga
     2221 agctcaggac cgtttccggc aatggtgatg gcccccgata gggcctctga cgctgagagc
     2281 aagacaccgg cgagcgtgat cggcaggcac aggtccgctc cacagacatg ccacggtcgt
     2341 ctccctctct tcggcttctc ataccaggga acgaattcca tctgtctcct ccgccgtggc
     2401 tatcgtctca acgaggccgc ccgtgttatg gcatcgagaa tcgctagtat gacaaacccc
     2461 aacaggaggc ccgcaggcca aaccgtgtcc cagtttcgca ttctgcctac ccccttccgc
     2521 atcacaccac cgccatcgcc cgcctgttct tccgatggac ctgacttcga cttagaattt
     2581 ccctctaccg agaccggcgg caggggaccc aggaacgcgc gcgcggctcc gaaccggctg
     2641 agagtgactc actcgattgg ctgcgatgcc gggatctgtc gcctccccgc acagaagaca
     2701 tcgccagcca gacactctgt tctggctcgt gatgtcttgc atttgaacga cgatcataag
     2761 accttggcag cgcagacaat gcatcgagtc ctccacaagt ccggatctac aaaagacttc
     2821 tcaagctctt ctagaagaag atgtgcgcac cggtcaatgc cgaatatctt cctgctcatc
     2881 cggaaacgac gcccaaatcg cgatgctcca catgatcacc aacaatccaa gcatactgta
     2941 aatctgatac atggcgctca cctcgctttc tatgaatcat tcagttcgct tccctcatcg
     3001 atgacgatcg cgtctcggaa tcggcgaccc tgtagggatg ccgattccga tcgggcgtat
     3061 cgtgaggacc gttcaaaatt taggagcgac gggggtcagt tgtctggtcc cctcttcgcc
     3121 tgcacaattt tgttcggcca acatgcgctg cccgacactg gcgatctcag gaattctcgc
     3181 cagacaggcc ttcagcgtat cctccactga accagatgcc accacatcca tcgctccgat
     3241 cggagcaacc accatggtgc cttctacgag cggatccgct gcatacgttg gcggcgtcgc
     3301 ccaggccgag atcgccagta cacacgagag cagcacaagt cgttgcatcg tcaaatggat
     3361 tctcatagcg tatccctcct cgaattgcgt aaggcccttg gttggcgctt catggcgggc
     3421 ccattccatg aagtgctgcg aaggatcatg tcgaatcacc gtatgccaga atagagcgtt
     3481 ggaacgtttt ctctgctctt gtacaaagac agaagagaca cccgtgccac gaaaccggcc
     3541 gttctcacat gagcccgatt gaattgcctc aaagagagtg gttgacggag cggcggagac
     3601 cgggaaatca gacgcgcagg caagagagcg atgcgcgagg gataggacag tcggcgtagc
     3661 tcaatacacg atgccttcac cctacagtgc ccgtatggga gagtcaatgt agggaggagg
     3721 tgaatacctg ctaagaagat gaaacaatac agttatattt atttcagtgc ggccggaccg
     3781 ctggcctccg gacagcaatg aatccttttc atcgaaagct ggctctcaag aggcgtgatc
     3841 accatgtaat taaggacgac gtatgtggac cgggaacatg acaggaagcg agcaacttgt
     3901 ccggactctc ctcgccctcg cgttcatcgg tttcagctcc caagagcaca ccacgctgat
     3961 tcaggccatg gccggctacg ttgtcggagg cattctcctc acaagcgccc tgttcgctca
     4021 atgcgccaca cggaaactcc tggagtagaa ttcatctctt gtcccatcaa gtgtacggcc
     4081 gtccaagcgc ctccgggaca cgtagatata gctcgctatc tgaacaattg ggtagtagag
     4141 tgatatcgag atgtcgagca gatatgggtc aatccctcgc atctctgtcc ggcgtgaact
     4201 caaatgattg atctcgtagc ttccgccgtt acttcctccg agttcctccg tccgtctcat
     4261 tgatcgatgt gtcattcgca gagggtcggc attcgcacct ccgtgaccga acattgcaga
     4321 acagctgtcc gacacaggaa ggaagcccca gtaccatgag caacccaata ccaaaccata
     4381 tgaagaccgc gagcatgtgg gtgccgatcc ccgatggcac cagggtcaaa ctccggaacg
     4441 aaacgctgga aggcgtgatc gacggtctga ccgagttcgt ctccggcccg gatcggaatc
     4501 cggacggaaa aacacaatac cggatgaata ttggaatccc gaccagacaa ctggtcgccg
     4561 aagacaacct ctccattctg ctcggtgggg atgatctggt catcatggtg cgacaaaagg
     4621 aaccctatcg tcgttctgtc acggaacaac tccgtggaaa attcgccgcg gatcggttca
     4681 agaaatccgc caagtcccac taggcccatg aacgtcggca agccgcacag atagaggaca
     4741 ctatggctga aacctgttac tcaaaaggca agaagatcat cctgctcccg gaacagaagc
     4801 ggaacggaac ctggacctgc cgctttatga ttccagggct gacggattcc atcctgcaca
     4861 gagcgcccga cacagccctt gcgacgtaca aatccgagtg ggaagcaaag acggctgcat
     4921 ttgcatccgc taaacacctg ctggatgcac aggcgtcacg aatagccagt cccactccag
     4981 agaggccttt atgagcaaaa cgacgttacg aaagcgccaa cgacagcaga cgcagcagga
     5041 gagacagcaa gagaaggcgg ttcgccggga acagcgcaac ctggagaaat ccgttctact
     5101 tcgatccgcc ggccaagaag acccggattt gaacggcatg gtcgcgggtc cccagcgtct
     5161 tcgcgactga gtgcgtccta tgaaggctcc aagtccctgt aatcaatgcg ttcctcgcga
     5221 gtagcttatt ctatgctgac gtgctagtat gtttatgttt cacggcggcg ggcctcgcgt
     5281 gaaacgggag atcgaacggc ccatcaccga tgttgttgtc accgagaatc tgattggaag
     5341 cctgacctaa gtcgcctacc tgatcgcgtc tttggccctc tccttcccct cgtgaagttg
     5401 ttctcgcgtc tcagacaatg tcattttcag aaggaggaac ccccatgggt tcaaaacttt
     5461 atgtcggcgg gttgccgttt gcggcaaccg aagcgcagct caccactttg tttgcggcac
     5521 acggcacggt tgaatccgcg cgcgtgattg cggacaagtt tacgggacaa tcgcggggct
     5581 tcggcttcgt cgaaatgtca accccggaag aagccaaggc agccattaca gccttgaacg
     5641 gatcgcaaat ggacggccgg tccctgaccg tgaacgaagc caagccacag gaacctcgtt
     5701 ccggtggagg cggcggtcgt ttcagcggcg gacgcggcgg taacgatttc ggcagccgca
     5761 gccgcttcta gtttcagatt cgacagcagg ggcgcctcat tgaggcgccc ctcccttcct
     5821 ccaacccaga gaccacacac catgcgcgac aaaacgattt cctcccaaga cattaggcgg
     5881 gcgcacccga attatatgac cacgatcctc ttcagcctcg tcgccggagg agcgctcatc
     5941 ggagtccctg tcttcggatt ggcgtatgga tatacctggc tggattggac gatgtttgga
     6001 gtcctatacg tgatgaccgg tttgggcatc acggtgggat accatcgcct gatggcacac
     6061 cgcagctttg actgcccggg ctgggtgaaa gcaggattct tagtcgcagg aggatgggcg
     6121 ctcgaaaatt ctgccctcaa gtgggccgcc gaccatatcc gccatcatgc cagctgcgac
     6181 caggaggccg atccttataa cgcgaagcgt ggattttggt atagccactg cgggtggctg
     6241 ttttttcacg atgtccatgc caatgagaaa tatgcgtcgc gactaagaca ggaccccatc
     6301 gtgatgtggc aacaccggta ctatttgccc attgtgctct cgggtctggg gctgacattt
     6361 gtcgtcggct ttctgcataa cggctggatc ggcggactcg gatgtttcct gcttgccggt
     6421 gtcggccgca cgtttgccgt gctgaactcg acattttgca tcaattctat ttgccatatc
     6481 tggggacgtc agcctcacgg ccaggccgat tccagccggg acagctggct ggtctcgctc
     6541 gtgacgtttg gcgaaggcta ccacaactat caccacacct ttcaatgcga ctaccgcaac
     6601 ggcccccgtt ggtataactt cgatccgtcg aagtggctca tctttgcgct ctcgaagctg
     6661 aagatggcct cctcactccg catcgcctct tccacccacg cctagaagcg ggtggctcgc
     6721 agtttccgaa gaaatatata aggaattgac gttcccgcag acgcttgatc agcgacgact
     6781 gcgagcgcgt atgtacgcag cgaatttgcg cagcccggac caagcctccg acccgaacga
     6841 ttccgcttat tccttcttct tcgctaccca gaatgcagtt caaaagaaca cgaaacgatt
     6901 ggagagattg cctaatcatg gcaggatgtg ggtcatggaa cggcagccgc acatgagtcg
     6961 cacccaacgc ttcttcctgt tcagtctact cgcaagcctg gcactcgcca ccctggtttg
     7021 caccccaagt tctgcagaat acccgacgct catcagcatc ggtccccgcg ttggtttcag
     7081 tcaaaaagct cccctattgg gacaacagca aaaatactac ttccacatga ctgacgtcgc
     7141 ggccgtcttc aggctcccct ggtcatggcc actcggtgag acctcatgga atctggagac
     7201 aagactgatc acaagtgcgg ggcttttgca agcgacaggt gacagcgctt tgatggtaac
     7261 gattgtgccg gatcttgcgc tgagcgcctg ggaggggctt atcaccttcg acgccggagc
     7321 aggggcaggt ttcttcagca actacaaatt cggcgagcaa gacttcggag gtccggttca
     7381 aattgtcgcg accttaggcg tcaccgtcaa tccattttct cacgcctatg ccggctttcg
     7441 cgcccagcac ttctccgacg ccggcgtgta cggcccgtcc agtctcggcg tggacatgta
     7501 catcgtggag ctcggctaca gattttgagg ccgggttcat cttgaccagc cgcttcgatc
     7561 atcaactaat tgaagccgta agtgtcttgc gcgagtacag gctatcctag actgtctccc
     7621 ccatcttctt tctccacccc gacaatacac accgttgcgc aatgagcggc acgaagctgt
     7681 cgatatcttc cgacactcgt gtcatcacct cgtttgcaag acgttgatca tcgaaacaat
     7741 caaacacgtc atcgagaaag ccccctcgca gcaacggatg gtagagaaat gctgcccctg
     7801 atccggttgc gacttcgagt ggatattcgg ccacaaccac ccgctcgagc cccgtgtcgt
     7861 acagccatct cctgccttct tccgcagacg gacgctctgc aatataggct tccagacggc
     7921 ggcgctctgt cagcaggcca cgaaccgcca tttcctggtc gatcctccgc cagagtgaat
     7981 caaatgtgcc cagtgaggga aacgtcagaa cgatttgacc tcccggttga agatgctgaa
     8041 tcaatccccg aatggaatcg agccggtccg gccgcatgaa catgacagac agattacctg
     8101 tgatgcggtc gaacagcgga agctcaggcg gcaacgcacg catgtccaga cactcgaacc
     8161 gcagccacgg gagttgtgcc ccttgaacgg ctcgcgcgct ggccacctgt ccttcgctga
     8221 gatcaatgcc gagaacctgt ccagcagcac ccacgcgctc tgctacatga aaggccggaa
     8281 tcccgtgacc agaggcgacg tcgaggattg tcgccccggg aaatagctca agtcgttgaa
     8341 gcaatgcttc ggcaaaggga gtggaccaat agtcgtgttg tggaagcgcc cagcgagtcg
     8401 tcacagcttg ctttcgttgg gttggcga
//
LOCUS       DATIFR010000118        25600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1802892, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000118 DATIFR010000000
VERSION     DATIFR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25600
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802892"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            622..5562
                     /locus_tag="VLD60_06785"
     CDS             622..5562
                     /locus_tag="VLD60_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44697.1"
                     /translation="MRAFLFQPTRKRRRTRAALMLQSVLLASSLVATPWLYAAGPGDQ
                     SHEGHATPVSMPGWTQQLKGQTVVENAIEGRAGNAEKMEMQHHRLMQKLEEQAQKDAK
                     AQQTSGGFNQMSMMHQYMGQDGSSFLLMTDAGKGEPVMTSGGKCPAEAPTKQFDVSMI
                     NIEITLNRWLDFYPGYMYVLTDDVKKVRAEEEKNKAARDKEGWDPGAVSTGLQGDAIQ
                     PLVLRANQGDCVKMTLRNQMEGEDGSLFIQASSMIVSATGKPATTTNPESIVAPGKSQ
                     EFEWYIHPHMQEGVRQFHSYSHDRELTVLGLFGAFVVEPKGSKYVDALGTGPDKEVKS
                     GWQVNIDNGTGPDFREFVLFYHEIGDEAFRPLNKKGDFLPQRDPLTDAYRPGGRALNY
                     RSEPFGIDQMHLQHEYFGFEDESLAYSAYTFGDVPTTIPRGYLGDPVKWRLVHGGSEV
                     FHSHHPHSGSIRWQRSPGTEPNNLWAMGKDGPVKYPIVRTKSDRVDVEVIGPSEALDL
                     EPECGGGGCQHLAGEFLFHCHVAHHYVAGMWGYARFYNTLQVGAAHTDTMPDLRELPD
                     RQGRMKLGVSSDKLIGTTLDWFGKTFKIVDKSQKTNWKSDPAVVNIKDWVEMWVPAAG
                     KPGHTEDEKGQIMAYDATVWDWKWNGNVAVGERESTAQNPKYQWAAKWDDSTRPAILF
                     DPTTGRMSWPLFKPHYGKRVPFSANHNGAPWLDPIHQDANGERTSEPAKPGENGRWSL
                     CPDNANQKFYNIHFVRMPITLAKKQGKEPAIVDKDGLIYVLHEEERLTRANDDLKFPA
                     VIRANVYDCVDLVLTSEWDDDDYTNFQSSKINIHPHFFQFDTGNSDGVISGFEYEMSV
                     RPFTMWGKPKKHGLPAPMVAKIVGGAKAGATSIKIQMAPGATAFHVNTELMIGMDCLE
                     KGNDAAASLPRDKSCAEVARIKDIKGDQVTFFKPLKHNHPANDLASPEFVRYRWWVDV
                     DMGTVFWHDHAFGATTWPHGGFGVTLVEPFGSTYHDPKNGKLVYSGPIADIHTNEPIG
                     AGVSGSFRELMVSIHDTVPHTVNVIQAGNPPGQPVEVALEAGKTVSFQMPDKIMNAPN
                     HYINGGTHTTGSGFNFRAEPFAQRLSNNPDTSKLFNSATHGDPDTPLLRAYTGDTMVF
                     RLLHQLMNESHVWTIAGHTFLTERYAADANRKNSIHVGIAERYDLVTKAGGFQGMPGD
                     YIHFNGRSSHFAEGGWGIVRVLDKETADLKPLPRGTNPLGIAATPSSVCPADAPVKSF
                     NVVALDRPLKLHPKAPETIEVDFERKIELTVPDGKIFALEEEATTVASGGTPHPLTLR
                     VNLGDCIKVNLKNKMKASRASFFAPGLAFDPKDSQGLNVGNNGGEQTVGAGESRAYTY
                     YAHPANKETTSLVWDGGNIIENPRNGLYGAIIVGPRGSQYRDPVTGADLSQKNAWRAD
                     VIVDASLPENVGKRNYRDVALFFQDEDNIIGTSFMPYVQNVAGLTSVNYRAEPYKFRE
                     EQGCSLGKIFQPCVVDKPEDPATPLIEAHAGDAIRIHVIGANSEQNGMFGLEGHEWPI
                     EPYMPGADMISVVEYAGSETLDVFIRGGAGGPYRQVGDFVWANARLPYSQSGQWGYLR
                     VLPAGDSRIQPLGAAGMGTKRAELELQPQAIPTAMK"
     gene            5728..6657
                     /locus_tag="VLD60_06790"
     CDS             5728..6657
                     /locus_tag="VLD60_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB44698.1"
                     /translation="MSIRNTMAAGVMAACLTSPANAYEEMAVTNGGTLSGTVSLVGDV
                     PKPKGYNLTTLPDQVYCGRISDGHGWRLLQPFKVDAKGGFRDVVIYIDEIEKGKPFHF
                     KIPKIEAIDCAFNPFMNVVWDKHQVEVVNMDPAMHDIQAYETSQLGPRVLFNVPLPMN
                     PRHPRDIKDTSEAARYHKHLAGTPMKQDVHMTKGRRVFVMQCGFHAYMESWGLAVENP
                     YYAVSDAQGSFTIDGIPPGTYKVTVWHPFIGGAKEYSVTIEPKAQAKLDVKIDAPVGR
                     LYANQMVENPYVRYTITDDAQIQIVPTLEKQTY"
     gene            6720..7715
                     /locus_tag="VLD60_06795"
     CDS             6720..7715
                     /locus_tag="VLD60_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSB44699.1"
                     /translation="MLRLPKNGGRIVKGKEMQGLRKLDWTVNVLLIVVALLTLAKVAW
                     GLDTQDIVVEWTEAGKKIAAERVANWKAKDDMVLVPAGEFLMGSDKKLDRNAYKAEMP
                     QRRVYLDAFEIGKYEVTALEYLKFVLATDRLPQLDWRYDGGNFQDTMAHHPIMHVTWY
                     DADAYCRWAGKRLPTEAEWEKAARGEDGRINPWGNQIAGLSRANFGRSGLSGPVRDRP
                     ERLLLYPPLISVDKYENAVSPYGLHQQIGNVAEWVADWYDQDYYKVAPDRNPKGPETG
                     KHRAFRGASWIDSSTTARAAQRNGADPNTKMNWLGFRCARDVQPSTGTQVSQVVP"
     gene            7839..8519
                     /locus_tag="VLD60_06800"
     CDS             7839..8519
                     /locus_tag="VLD60_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44700.1"
                     /translation="MQGPRTGWSIGVKSVAAAIAVALAVCGAWPASAEEGTGNMVFFK
                     GGYARMDSNRAGETFSDCGLTSTTCGQNNSHDGWYVGAGLDLVLSKNVWGGMDKTWVM
                     GEIGMQFNRFSSSTVTGGVSALSAAPAKTQLTSVTIDVAPKIKFMEGSALRPWVIPAG
                     LDIMVVSPPSNQTQYLDVGVQFGAGVEYEIWKAFKLGLDTRYHLMANMTNTSNSYFQV
                     GPYVGISF"
     gene            8703..9260
                     /locus_tag="VLD60_06805"
     CDS             8703..9260
                     /locus_tag="VLD60_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44701.1"
                     /translation="MHLVVHIPYQADEESTRALSLARQAPSFTMEWDDDHKVAIAIYS
                     SLPKGIEEAVQLVGEAVRLSGAWASMDSRRVSSLTKLWQRLACYQGSLDVEDPLQYCK
                     ERSVLFNVLVGCEGQRCPVPCQFICTPCMRMEQEETAVRSGDQYKVAAELAEIDWCPR
                     LRIPYEEHSTLIDPSSLKPPVEPSL"
     gene            9332..9751
                     /locus_tag="VLD60_06810"
     CDS             9332..9751
                     /locus_tag="VLD60_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44702.1"
                     /translation="MVFLSLVLAPLVRSRNSTTEIVALFRSAALRFRLVVWGAMAVLL
                     ATGPVLLSQRQIAISNPAAWPEVLVVKLGLVAGLLLSTFLHDLFLGPRVSRIAAISES
                     TRTAWEQTLVKTARWLPRLSIFIAMAVIVVAVMLART"
     gene            9950..10378
                     /locus_tag="VLD60_06815"
     CDS             9950..10378
                     /locus_tag="VLD60_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HSB44703.1"
                     /translation="MHPIHPMAVHFPIALLSTTVLFDLLWSRWQLDGLRAAGWYTLVL
                     GLGGALVAVITGSIAEEGVEHSGVPKRVLEIHETLGFATFWIFAGLLGLRIAEWLGWI
                     RERLALRIVLGLGAVALLFIASYYGGSLVYEFGAGVALPR"
     gene            10405..10872
                     /locus_tag="VLD60_06820"
     CDS             10405..10872
                     /locus_tag="VLD60_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44704.1"
                     /translation="MNRGLCVTVAALWVVTVIAAGWFFVKGWTTKGTDGRIEIVLAMT
                     ERDQILAEMRQLLKAVDSIVRELGEPQPDLRMIEEATRKAGMKMAADVNPAIMAKLPL
                     PFKQTGMSIHKDMDALADAAVQQETPQQLLQRLSSMTARCTACHDLYRFSLER"
     gene            10978..12225
                     /locus_tag="VLD60_06825"
     CDS             10978..12225
                     /locus_tag="VLD60_06825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HSB44705.1"
                     /translation="MARVVIIGASIGGLPAAYEAQALLGKKHKVTVISNVDSFHFVPS
                     NPWVAVGWRKRKDISFALGPVLEKKGIEFIHATADRIEPEQNRVVTAKGEVPYDYLIV
                     ATGPKLNFGAVPGLGPSGYTQSVCNVDHAEQAWGAYQSFLRDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKKKLRKKVPIYFVTPEPYIGHMGLAGVGKSRRLMEDEFAEHSIK
                     PIYNTAIKEVQPGKMILEDGQEVPFRYSMIIPPFAGVDAVAGTPGLCNPKGFVNIDAY
                     QANPKYKNIYAVGVCVAIPPVEQTPVPTGAPKTGYMIESMVSASVHNIKADIENNDKK
                     DTATWNAICLADMGDTGMAFVALPQMAPRNVTWAKKGKWVHLAKIALEKYFLMKMKRG
                     VSEPFFEKAILKAMGINKLEKAG"
     gene            12301..12489
                     /locus_tag="VLD60_06830"
     CDS             12301..12489
                     /locus_tag="VLD60_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSB44706.1"
                     /translation="MKLNELLRLIAGVFVLLAVFLGATVHPYWNYFAAFVGLNLIQSA
                     FTGWCPMMALLRKLGVQE"
     gene            12492..13646
                     /locus_tag="VLD60_06835"
     CDS             12492..13646
                     /locus_tag="VLD60_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB44707.1"
                     /translation="MAARSLPYTGGMRTLRRTSLGAHSRGLMHVTIAPATRWLIVACL
                     LLSAGCGSKEEPTTSGLSAAPRATIQAAVVEIKTVRVPVRVEVTGQVATVYQATLSSR
                     IQGTIDKLLVREGTQVSKGQTLVQLDSRDVQADLARASAEVENARAHFNRMKALLDDQ
                     AVSTQEVENATRALKVAEANRKAVLAQLSYTVVKAPFDGVITEKKVEAGELASPGQPL
                     LKMEDPRQLRLEATVAEGDLKAISRGDKISVVIDALGAQALRGIVSQILPAGDPQTHT
                     FMVKVDLPSTPGLKTGMFGRFQLDKGTAQTILVPSSAIVERGELTSLFVVGADQVARL
                     RWVKVGRRFEKQIEILSGVNVDERVLVDGSRGVDGAPVQIVNTVAVPAVP"
     gene            13656..16931
                     /locus_tag="VLD60_06840"
     CDS             13656..16931
                     /locus_tag="VLD60_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB44708.1"
                     /translation="MDQYRPGLSGRIAALFIGSKLTPLIMLGTLLLGLFAIVVTPREE
                     EPQIIVPMADVWLPFPGASAKIVEEQLTKPFERKFSEIKGVEYVYSISRPGGALIIVR
                     YFVGQPMEQSLVDLYDKLMSNQDLLPPGAETFLVKPKDVNDVPIVTVTLSSQRYGEYD
                     LYQLAEQVLEEAKKVPGTSAGFIVSGQARELRVRIDPARLKAYGLTPLQIAGVIRAEN
                     RALPTGRFDSRNQSYLVETGRFIRSREDLEALVVGVHEQRPVYLRQVADVTDGPRESN
                     QYVWFGLGPGDASEGAGARGEGKASEAAPDTSRLMPHESPAVTVAIAKQGGTNAVTVA
                     SEVIRKVEGMKGVTIPADVRVTVTRDYGETAQEKANELLWHLLIAVVAVVGFLGVALG
                     LRPAIVVSLAIPLTLALTLFTSMLIGYTINRVTLFALIFSIGILVDDAIVVVENTYRH
                     LTLHVKPHHEASIVAVDEVGNPTILATLTVIAALLPMAFVSGLMGPYMRPIPVNASIA
                     MFFSLLVSFIVIPWFCQTCYRTLGGNSALSTQHSDLDERGLMARVYRRAVSPLLAHPI
                     LASAFFGIVVLLLIGSAALFYTRHVVVKMLPFDNKSELQLVVDMPEGTTLEETARVTQ
                     ALGRYVRTLPEVRDYQAYVGTASPFNFNGLVRHYYLREQPHEADIQINLVPKHERAAQ
                     SHEMAQRIRPQVQEIAKQYRANVKVVEVPPGPPVQSVLVGEIYGPDYGRQIAVAREVR
                     ALFESTPGVVDVDDYIEADQVKYIFTVDRAKAALAGIPTEDVVKTLRMALEGTKIGLV
                     HIPEEKSPVQIVLRLPLAERTGLGSIGEIGLRTNTGGIVQLSELLTVEQTIQDKAIYH
                     KNQKPVVYIVADVGGPGAEKAESPVYGVLGVGKKLEGFRPAEGYQIEQYYASQPWSED
                     KFGMKWDGEWHITYETFRDMGIAFAVAMLLIYLLIVGQFQSFLTPLIIMAPIPLTLIG
                     ILPGHWLTGSYFTATSMIGFIALAGIIVRNSILLVDFIQLQEQAGVSLSDAVIKAGAI
                     RTRPILLTAAALMVGAFVIILDPIFQGLAVSLLFGVGVSTLLTLIVIPVAYYHVMGRR
                     TAS"
     gene            18100..19209
                     /locus_tag="VLD60_06845"
     CDS             18100..19209
                     /locus_tag="VLD60_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB44709.1"
                     /translation="MRNPKRDALIVTGLVALLGGGYLVAWTGFWNRGLPEGLIQVNGR
                     MEGDHLTIASKFPGRIQELLVREGDNVTTGQIMVRIDDVQTRARVDQTQQAFAAIEAQ
                     VQAAHTTLAVLNLEVPLAIESAQSHVDEDRAELEKAKTVEREARMDEQRFRNLLPEQA
                     VTQQQYEQALARWNVARSQVVAARSALAKATKELAVAELGWKRIRAKEDEVAALERQR
                     DQAEAALSETESVLADLTIRAPANGTITTRMVDIGEVVTAGAPLFEVVDLDRLYLKVY
                     VPEIQIGKIRLNLQARIYTDAFPDRPFDATVRYIASTAEFTPKEVQTPDERVKLIYAV
                     KLYVKENPDHRLTPGLPADAVIRWKEDVAWVPPRR"
     gene            19309..21267
                     /locus_tag="VLD60_06850"
     CDS             19309..21267
                     /locus_tag="VLD60_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB44710.1"
                     /translation="MSRDSTIVHVAQFVKRYKKHVAVNGVDLTIKKGEIYGLIGPDGA
                     GKSSLMKAVAGVLSYDGGAVEVFGTLVDSERAAEQVKQRLGFLPQGLGLNLYPDLSVE
                     ENIDFFARLRLVPDRELAERKDRLLAMTRLDKFRDRAMKNLSGGMKQKLGLICTLIHE
                     PELAILDEPTTGVDPVSRRDFWAILAELLQEKGMTALVSTAYMDEAARFHHLSFLSHG
                     RVLTAGTPAEVQALVPGSMMTFEATPQLEAVARLQRRYKQVEALGSKLHVFTDETDRA
                     QAVKSLETNLGDIKPEQVRVGEPELEDVFVALLLRQATGPEVPALPATPGQASNSGLA
                     IEARELIRDFGTFRAVDRVSFRVKPGEIFGLLGANGAGKTTVIKMLNGILSPSGGEGW
                     VAGADMRKAGGAIKERIGYMSQAFSLYLDLTVEENIRLFAGIYGLDRQAANERLAWIM
                     SMGGLKGYESSLTGRLPMGVRQRLALGCALVHRPRVLFLDEPTSGVDPIGRRHFWDIL
                     SRLAREEGVAILITTHYLSEAEHCDHLALMFAGRIVAEGSPAAMKDQVEHEAGHLLEV
                     ATDQPGAALRRLTQAGFTDAALFGTKIHLFSIDPAQDEVRLRESLASDGVHILAMNVR
                     PLSLEDVFVHRVMALERQEREATTGAVA"
     gene            21264..22418
                     /locus_tag="VLD60_06855"
     CDS             21264..22418
                     /locus_tag="VLD60_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB44711.1"
                     /translation="MNLRRIAAVSSKEWREVIRDRMFVLLAFLMPVVWMLVFGYGLVL
                     DVENISFVVVDRDHSSLSREYLYRFVQSRYFNFQGELAAERDADVWLQSGKARAVIIL
                     PEKFEERLLSGQPANVQTLIDGSFPLRADITKGYIIAINSAFNEGLLVDHLARTRGLS
                     RDEAQRRLRPLALEVRYLYNEEVRSAWSTVPALIMVSLMVSSPLLTALGIVREKETGS
                     IYNIYSATVSRLEFLAGKLFPYVMISAVNAMVLWLMAVFLFAVPFKGNLLLFFATSLL
                     FVLTSTGIGLVVSLLVSTQQAALIITILLSTVPTILFSGLVVPVSSLSQAAQFQAHLF
                     PGMYYTNIVRGTFLKNIGPAVLWPDILALGAYAAVLGTIGYRLFTKRPRA"
     gene            22415..23572
                     /locus_tag="VLD60_06860"
     CDS             22415..23572
                     /locus_tag="VLD60_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB44712.1"
                     /translation="MKPAARIAVWCRRVAVMTRKELMQLFRDIPLIAFLVYSFTFAIF
                     VTGNGIKTQLRNAGLVVYDADRSVSSRELVSRFHLPYFRFDGELAHPDEELRRLDRGS
                     SMVVLDIPARFHEALAAGEPTAVQLLVDTTNSPQGLSAAGYAARIVGQFGQEVALARN
                     GLADAYLTSVPMILSDHRVWFNQDQNETWFESISHLLRMITIFSVLLPAAALVREKER
                     GTVEQLLVSPLTPVQIILPKVVAMMLVILCATAVGLFGVMRPAFGVPIKGSIGLLFSL
                     TALFIMATAGMGVFAATVTRNQAQVGMLTLLAVAPMLLLSGIFTPLEMMPAWVRHLMA
                     FSPLRYFIEIVNGILLKGIGIEMLWTQTLSMGLVGGSLFGFGLWRFRRQFQ"
     gene            23774..25576
                     /gene="ftsH"
                     /locus_tag="VLD60_06865"
     CDS             23774..25576
                     /gene="ftsH"
                     /locus_tag="VLD60_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSB44713.1"
                     /translation="MNKKVSFSIWYIILAIWAVILVHDFISAVQKIEEFPYSEFKTLV
                     AAGKVAEVSVSSHMLTGKLKPEGESKEQKQFTSIRVEDPELVRDLEQHGVKFTGVIET
                     TFWRDLLSWLLPVLVFVGIWFFIFRRLGQAQGGFMQVGQSKAKIYMEKDIKITFADVA
                     GVDEAKEELREVIEFLKTPDKFTKLGGKIPKGILLVGPPGTGKTLLARAVAGEAGVTF
                     FSISGSEFVEMFVGVGAARVRDLFEQAKLKAPCIIFIDELDALGKARGMGPMAHEERE
                     QTLNQLLVEMDGFDPRIGVILMAATNRPEILDPALLRAGRFDRHVLVDRPDKIGRLAI
                     LRVHAKQVALGPDADLENIAAMTPGFSGADLANIINEAALLAVRRGKDQVGISELREA
                     VERVIAGLEKKNRVLNKMEKERVAYHETGHALVAMSLPGSDQVQKISIIPRGIAALGY
                     TLQLPTEDRFLMTKSELENKVAVLLGGRIAEELIFGEASTGAQNDLVKATDIAKSMVK
                     AYGMSEKLGTITLERERQPQFVQIQMAQEKGDYSEETAREIDCEVRRIVDEQYERVNK
                     LLGERKAALQEGAKLLLEREVISGVELKAIMEKT"
BASE COUNT         5448 a         6848 c         7935 g         5369 t
ORIGIN      
        1 gccgtcactc aggctgaacg cgtgcgcatt cttgaagcat tgaaaaagac cggcggaaac
       61 cgcgtgagtg cggccaaatt gctcaagatc agccgcgcca gtctctacaa caaactccgc
      121 gactaccgac tcgaataacc catctctcgc tcctgtttgc ccctctccgc ctcaattccg
      181 ccctgcaaac tgtccaaact gttagacagt acaagtcatt gaaagatcgc acgtcagttt
      241 ttgtcgtact tccagctgtc taactcggta gaaattagcg tcatgttcga ttctaacatc
      301 ccgagcctct tttcttgcgt aactcatgtc cccagcgaat caatttgaca tttcgtggat
      361 cgatcccaat ctcggcacag ctgttgcgct tgtgaagcag cgctggtgaa ggtctgggtg
      421 attaggaccg gcctccgaat gacccactcc ttcgcaagaa ggaacccttc accagctcta
      481 atccctccag aatcagggcc gggggtcagt cggaggccgg ttctcttctg aacgaggaac
      541 caggagacag agtgaggatc aagaggcagt gtgtttcaac catccgccaa atgaggcggg
      601 cataacaggg ggaggccgtt tatgagggct tttctatttc aaccaacacg caagcggagg
      661 cgcactcgtg cggcgctgat gcttcaaagc gtgctgttgg caagtagcct ggtggcgaca
      721 ccatggttat atgccgcagg gccgggtgat cagtcgcacg aagggcatgc gacgccggtc
      781 tctatgccgg gctggaccca gcaattgaag gggcagaccg tcgtggagaa cgccattgaa
      841 gggcgtgcgg gcaatgcaga gaagatggag atgcaacacc atcgtctgat gcagaagctt
      901 gaagagcagg cgcagaaaga cgccaaggcc cagcagacgt ccggcggatt caaccagatg
      961 tccatgatgc accagtacat ggggcaggac ggcagcagct tcctgctcat gacggatgcg
     1021 ggcaaaggcg agccggtgat gacctcgggc gggaaatgcc cggccgaggc cccgacgaaa
     1081 cagtttgacg tgtcgatgat caacatcgaa atcacgttga accgctggct cgatttctat
     1141 cccggctaca tgtacgtgct gacggatgat gtcaagaagg tgcgagcaga agaagagaag
     1201 aacaaggcgg cgcgcgacaa agaggggtgg gatccaggtg ccgtcagcac cggcctccag
     1261 ggcgatgcga ttcagccgtt ggtgctccgc gctaaccagg gcgattgcgt caagatgacc
     1321 ttgcgcaatc agatggaggg tgaagatggc agtttgttca tccaggcctc cagcatgatc
     1381 gtgagcgcca cgggcaagcc ggcgacgacg acgaatcccg agtcgattgt ggcgccgggc
     1441 aagtcgcaag aatttgaatg gtacatccat ccgcacatgc aggaaggtgt gcgacagttc
     1501 cactcctaca gccatgaccg cgaattgacc gtgctcggtc tcttcggcgc gttcgtcgtc
     1561 gaaccaaagg gttccaagta cgtcgatgcg ttggggaccg gtcccgataa agaggtcaag
     1621 agcggctggc aggtcaatat cgataacggg actggcccgg atttccgcga gttcgtcctg
     1681 ttctatcacg agatcggcga tgaagcgttc cgtccgttga ataagaaggg cgacttcttg
     1741 ccgcagcgtg atccgttgac ggacgcctat cgtccggggg gccgcgcctt gaactatcgg
     1801 agcgagccgt tcggtatcga tcagatgcac ttgcagcacg aatatttcgg tttcgaggac
     1861 gagtcgttgg cctatagcgc ctatacattc ggcgatgtgc cgacgacgat tcctcgcggg
     1921 tatctcggtg atcccgttaa gtggcgtctg gtccatggtg gatcggaagt gttccactcc
     1981 caccacccgc acagcgggtc gatccgctgg caacgcagcc ccggcactga gccgaataat
     2041 ctctgggcaa tgggtaagga tggaccagtg aagtatccga tcgtccgaac caagtccgat
     2101 cgcgtggatg tggaagtcat cggcccgtcc gaagcgttgg acctcgagcc ggaatgtggc
     2161 ggcggcggct gtcagcacct ggccggcgaa ttcctctttc actgccacgt cgcgcaccac
     2221 tatgtggcgg ggatgtgggg ttatgcccgc ttctacaata cgctgcaagt tggcgcggct
     2281 catacagaca cgatgccgga tttacgcgaa ttgccggatc gtcagggccg gatgaagctc
     2341 ggtgtctcgt cagacaagct gatcggaacc accctcgatt ggttcggcaa gacgttcaag
     2401 attgtcgaca agagccaaaa gaccaactgg aaatccgatc ctgcggtcgt gaacatcaag
     2461 gattgggtag agatgtgggt gccggcggcc gggaagccgg gccatacgga agatgaaaag
     2521 ggccagatca tggcctatga tgcgaccgta tgggactgga agtggaacgg gaacgtggcg
     2581 gtcggcgaac gggaaagcac ggcgcagaat cccaagtatc agtgggccgc caagtgggac
     2641 gacagcacca gaccggcgat tctgtttgat ccaacgacag ggagaatgtc ctggccgttg
     2701 ttcaagcccc actacggcaa acgtgtgccg ttctctgcga atcacaatgg tgcaccctgg
     2761 ttggacccga tccaccagga tgcgaacggt gaacggacct ctgaaccggc aaagccgggt
     2821 gagaatggcc gttggagcct ctgtccggat aacgccaatc agaagtttta caacatccac
     2881 tttgtccgca tgccaatcac cctggcgaag aagcaaggga aagaaccggc catcgtggat
     2941 aaagacggtc tgatctatgt gctccatgaa gaggagcgcc tgaccagagc gaacgatgat
     3001 ctcaagttcc cggcggtcat ccgcgccaac gtgtatgact gcgtggactt ggtgctcacc
     3061 agtgagtggg acgacgacga ctacacgaac ttccagtcgt ccaaaatcaa tatccatccc
     3121 cacttcttcc agttcgatac agggaactcg gatggtgtga tctccgggtt cgaatatgaa
     3181 atgtcggtcc ggccgttcac catgtggggc aagcccaaga agcacgggct gcctgcgccg
     3241 atggtcgcca agatcgtcgg tggtgcaaag gcaggggcca cgtcgatcaa gattcagatg
     3301 gctccgggcg ccacagcatt ccacgtcaac accgagttga tgatcggtat ggattgtttg
     3361 gagaagggca acgatgccgc ggcgtcactg ccgcgggata agagctgcgc cgaagtcgcc
     3421 cgcatcaagg atatcaaggg cgatcaggtc acgttcttca agccgctgaa gcacaaccat
     3481 ccagccaacg atctcgcctc gccggaattt gtccgctatc ggtggtgggt cgacgtggat
     3541 atggggaccg tgttctggca cgaccatgca ttcggcgcga cgacctggcc gcatggcggg
     3601 ttcggcgtga cgctggtcga gccgttcggg tccacctacc acgatccgaa gaacggcaag
     3661 ctcgtgtaca gcggtccgat tgcggacatt catacgaatg agccgatcgg cgccggtgtc
     3721 agcggcagct tccgtgagtt gatggtttcc atccacgaca cggttccgca taccgtgaac
     3781 gtgatccagg ccggcaatcc tcccggacag ccggttgaag tggcgctgga agcaggcaag
     3841 accgtgtcgt tccagatgcc ggacaagatc atgaatgcac cgaatcatta catcaacggc
     3901 gggacgcata cgaccggcag cggatttaac ttccgcgcgg aaccgtttgc gcaacgccta
     3961 tcgaacaatc cggatacgtc caagctgttc aacagcgcga cccatggtga tcccgacacg
     4021 ccactcttgc gggcgtacac cggtgacacg atggtgttcc gtctgttgca ccagctgatg
     4081 aatgaatcgc acgtctggac gatcgccggc cataccttcc tgacggaacg gtatgcggcg
     4141 gatgccaatc ggaagaactc gattcacgtc gggatcgccg agcggtacga cctcgtgacc
     4201 aaagcaggcg gattccaggg catgccgggc gactacatcc acttcaacgg ccgaagctcg
     4261 cacttcgccg aagggggatg gggtatcgtg cgcgtgctcg acaaggaaac agcggacctc
     4321 aagccgcttc cgaggggaac caatcctctt ggaatcgctg ccacgccgag ttcggtgtgt
     4381 ccggctgatg ccccggtgaa gagtttcaac gtggtggcgt tggatcgccc gctgaagctc
     4441 catccgaagg cgccggagac gatcgaagtg gacttcgaac gaaagatcga gctgaccgta
     4501 ccggacggca agatcttcgc gctcgaagag gaagcgacga cggtggccag tggtgggacg
     4561 ccgcacccgc tgacgctgcg cgtcaacctt ggtgactgca tcaaggtgaa tctcaagaac
     4621 aagatgaagg ccagccgggc gtccttcttt gcgccgggct tggcgttcga tccgaaggac
     4681 agccaaggtt tgaacgtcgg caacaacggc ggcgagcaga ccgttggtgc tggtgaaagc
     4741 cgtgcctata cctactatgc ccatccggcg aacaaggaaa cgacgtcgct ggtgtgggac
     4801 ggtgggaaca tcatcgagaa tccgcgcaac ggattgtacg gtgccatcat tgtcggcccg
     4861 aggggatcgc agtatcgtga tcccgtgacc ggcgccgatc tctcgcagaa gaacgcgtgg
     4921 agagcggacg ttatcgtgga tgccagcttg ccggaaaatg tcggaaagcg gaactaccgc
     4981 gatgtggccc tcttcttcca ggatgaagac aacatcatcg gaacctcgtt catgccctac
     5041 gtgcagaacg tggccggttt gacgtcggtg aactaccgcg ccgaaccgta caagttccgt
     5101 gaagagcaag gctgctcgct cgggaagatc ttccagcctt gcgttgtgga caagcccgaa
     5161 gatccggcca cgcctctcat cgaggcccac gccggtgacg cgatccgcat ccacgtgatc
     5221 ggggccaaca gcgagcaaaa cggaatgttc ggcctcgaag gccatgagtg gccgatcgag
     5281 ccctacatgc cgggtgccga tatgatcagc gtggtggagt atgccggatc cgagacgctc
     5341 gacgtcttca tccgtggcgg ggcaggtgga ccctaccgcc aggtgggaga tttcgtgtgg
     5401 gcgaatgccc ggctgcccta ctcgcaatca gggcaatggg gctacctccg tgtattgccg
     5461 gccggcgatt cacggatcca accgttgggc gctgccggta tgggcaccaa acgggcggag
     5521 ttggaactgc agccccaggc tattccgacg gcgatgaagt agccgatcgc agaatagcgt
     5581 aggtccaaga cgggggagct gcgccgatgg tgtagctccc ccgttgctta ggaagaagct
     5641 ctcagccgtc agctatcagc atgttgggtt gtcaggctga ctgctgaagg ctaagtgctg
     5701 atagctactg aaagaggaga ggccggaatg agcatcagaa acacgatggc tgcaggtgtc
     5761 atggcagcct gcttgaccag tccagccaat gcctatgaag aaatggccgt gacgaatgga
     5821 gggacgctct ccggcaccgt gtcgctggtc ggggacgttc ccaagcccaa gggatacaat
     5881 ctcacgaccc tgccggatca agtgtactgt gggcgcatct ccgatgggca cggctggcgg
     5941 ctgttgcagc cgttcaaggt cgatgccaag ggcggatttc gagacgtcgt gatctatatc
     6001 gacgaaatcg agaaggggaa gccgttccat ttcaagatcc ccaaaatcga ggcgatcgac
     6061 tgtgcgttca accccttcat gaatgtcgtg tgggacaagc atcaggtcga agtcgtcaac
     6121 atggatcctg ccatgcacga catccaggcg tacgaaacgt cgcagttggg accgcgcgtg
     6181 cttttcaacg ttcccttgcc gatgaatccg cggcatcctc gcgacatcaa ggataccagc
     6241 gaggcggccc gctaccacaa acatctggct ggaacgccga tgaagcagga cgtgcacatg
     6301 accaaaggcc gtcgtgtttt cgtcatgcag tgcgggtttc atgcctacat ggaaagttgg
     6361 gggcttgccg tcgagaatcc gtactatgcc gtcagcgatg cgcaggggtc gtttacgatc
     6421 gacgggattc cgcctgggac gtacaaggtc acggtgtggc atcccttcat cggcggcgcg
     6481 aaggagtaca gcgtgacgat cgagccgaag gcgcaggcca agctcgatgt caagatcgac
     6541 gcaccggtgg ggcggctcta cgccaaccag atggtcgaaa acccgtatgt gcggtacacg
     6601 atcaccgatg atgcgcaaat ccagatcgtg ccgacattgg agaaacagac gtattgaagc
     6661 tctcatcgat cagccctcag cttgtaaagg tctattcgtg gctgacagct gaaggctcaa
     6721 tgctgagact tcccaagaac ggaggccgaa tcgtgaaagg caaagagatg cagggattgc
     6781 gaaagctcga ttggaccgtc aacgtcctct tgatcgtcgt tgcgttgctg acgctcgcca
     6841 aggtggcatg gggacttgat acgcaagaca tcgtcgtcga atggacggaa gcgggcaaga
     6901 agatcgccgc tgagcgcgtg gccaactgga aggccaagga tgacatggtg ctggtgccgg
     6961 cgggcgagtt cctgatgggc agcgacaaga aactggatcg gaatgcctac aaggccgaga
     7021 tgccgcagcg acgcgtttac ctcgacgcgt tcgaaatcgg caagtacgaa gtcaccgcgt
     7081 tggaatacct caagttcgtc ttggccacag accggttgcc gcagctcgac tggcgctacg
     7141 atggcgggaa tttccaagac acgatggcgc accatccgat catgcatgtg acgtggtatg
     7201 acgccgatgc ctattgccgc tgggccggca agcgcttgcc gacagaagcc gagtgggaaa
     7261 aagccgcgcg tggcgaagat ggacggatca atccctgggg caatcaaatt gccggactca
     7321 gccgggcaaa tttcggccgc tcggggctgt ctggtccggt gcgcgatcgc ccagaacggt
     7381 tgctgctcta cccgccgctg atttcagtcg acaagtacga aaacgcggtg agtccctacg
     7441 gtctccatca gcagatcggc aacgttgccg agtgggtggc tgattggtac gaccaggatt
     7501 attacaaggt ggcgccggac cggaatccca aaggtcccga aacgggcaag catcgtgcgt
     7561 tccgcggagc cagctggatc gacagcagta ctacggcgcg cgccgcccaa cgcaacgggg
     7621 cggaccccaa cacgaagatg aactggttgg gcttccgttg tgcgcgggac gtacaaccca
     7681 gtacggggac ccaagtgtcc caggtggtgc cgtagcaggg aggtgttgtg tgattcgatt
     7741 gttcaccaag ctgatgtctc gggccggcga gcgcaagtca gctcaactat cggaagatct
     7801 gaacactctt ccgatgatcg cccaggaggg gggacatcat gcagggaccg agaacagggt
     7861 ggagcattgg agtgaagtct gtcgcagctg cgatcgccgt ggcgctcgct gtctgtggtg
     7921 cgtggccggc ttcggctgaa gaaggcaccg ggaacatggt gttcttcaaa ggtgggtacg
     7981 cgaggatgga cagcaaccgc gcaggggaaa cgttctctga ttgcggattg acttccacga
     8041 cctgcggcca aaacaacagc catgacggct ggtatgtcgg tgcagggctc gatcttgtcc
     8101 tttcgaagaa tgtctgggga gggatggaca agacttgggt aatgggggag atcgggatgc
     8161 agttcaatcg gttcagttcg tcgaccgtga caggaggtgt ctcggctctt tcggcggcgc
     8221 ctgcaaaaac gcaactcacc tcggtgacca ttgatgtggc cccgaaaatt aagttcatgg
     8281 aaggaagtgc cttgcgtcca tgggtcatcc ctgctggcct ggacatcatg gtggtcagcc
     8341 cgccatccaa tcagacgcag tacttggatg tgggtgtcca gttcggagcc ggtgtggagt
     8401 atgaaatctg gaaggcgttc aagcttgggc tggacacacg ctatcacctg atggcgaaca
     8461 tgacgaatac cagcaacagt tactttcagg ttggtcccta cgtgggaatc agtttctaag
     8521 cgctgacagg ccggcgaccg gttcttcctg tgagacatga cacgtcgaag gtgaggtcta
     8581 ggaacagggc gcaagcccag gagtccgaga cttcacctga ttgcctcaag tggtttcatt
     8641 ctcggaatgt gacgagaacg tttaggtagt agcctgctcc aatctgttgc ttgtgagagt
     8701 cgatgcatct cgtcgtacac attccctacc aggctgacga agaaagtacg cgggcgttga
     8761 gcctcgctcg acaggcgccg agcttcacca tggagtggga cgatgaccat aaagtcgcga
     8821 tcgcgatcta ctcctcgctg ccgaagggaa tcgaagaggc ggttcaactc gtcggcgagg
     8881 ccgtgcggct ctccggagcc tgggcgagca tggattccag acgggtctcg agcttgacca
     8941 agctctggca gcgcctcgcc tgttatcagg gaagtttgga cgtcgaggat ccgttgcagt
     9001 actgcaagga aagatcggtg ctgttcaacg tcctcgtcgg atgcgaggga cagcggtgtc
     9061 cggtaccttg ccagtttatc tgtacaccct gcatgaggat ggagcaggag gagacggccg
     9121 tgaggtctgg tgatcaatac aaggtagctg cggagctggc tgaaattgac tggtgccccc
     9181 gattgcgaat cccatacgag gagcactcca ctcttatcga tccaagttca ttgaagcccc
     9241 ctgttgagcc ctctctctaa tcgtcatgtc tcctctttgg atcctggcca tctctctcca
     9301 tattcttgcc gctgtcgcct ggattggagg catggtcttc ctctctttgg tcctggcgcc
     9361 tcttgtgagg agccgcaatt cgacgaccga gatcgtcgcg ttgtttcggt ctgcggcatt
     9421 gcgctttcga ctggttgtgt ggggtgcgat ggcggtgctc ctcgcgaccg ggccggttct
     9481 gctgtcgcag cgacaaatag ccatcagcaa tccagccgcc tggccggagg tcctggttgt
     9541 gaaactggga cttgtggcag gacttctcct gtccaccttt ttgcatgatc ttttcctcgg
     9601 gccaagggtc agccggatcg cggccatatc ggaatccaca agaacggcgt gggaacagac
     9661 ccttgtaaaa acagcccgct ggttgccgcg cctgtccatc ttcatcgcaa tggcagtgat
     9721 cgttgtcgcg gtgatgctcg cgcggacata ggtctcatgg ctctgtcgcg ttctgcgaca
     9781 gtggcaaatt cctctctgta gcgtgcttcc actgcgtttg tccgattcct tgtgctcctc
     9841 ataccgaact agtcaatttc attatagaaa tatcgtgcaa ggagctttat tcatcgaggt
     9901 ttcctctttg caaccatgcc attgggatct ttgaacaagg agaggaacca tgcacccgat
     9961 tcatccaatg gccgttcact tcccgatcgc cctgttatcc acaacagtac tgttcgatct
    10021 tttatggagt cggtggcagt tggacggact tcgggcggca ggctggtaca cgttggttct
    10081 tggtttgggg ggagcactcg ttgccgtcat caccgggtca atcgccgagg aaggggtcga
    10141 gcacagcggc gtaccgaagc gggtcttgga aattcatgaa acgttgggct ttgccacatt
    10201 ctggatcttc gcgggcttgt tggggctgcg cattgccgaa tggctagggt ggattcgcga
    10261 acgacttgct ctgaggattg tcctaggctt gggcgcggtc gcgttgctgt tcattgccag
    10321 ttattacggg ggtagcctgg tctatgagtt cggtgcgggg gtcgccctgc cgcggtaggc
    10381 ggaatggcaa ttcgaggaga aaagatgaat cgaggcctat gcgtcacagt ggcggcactg
    10441 tgggtcgtca cggtcatagc cgccgggtgg ttcttcgtga agggatggac aaccaaaggg
    10501 actgacggcc gcatcgaaat cgtattggcg atgactgagc gggatcaaat tcttgcagag
    10561 atgcgccaac tcttgaaggc ggtggacagt atcgtcagag agctcggtga acctcagccg
    10621 gatctgagga tgatcgaaga agccaccaga aaggccggaa tgaagatggc tgccgatgtg
    10681 aatccggcga tcatggcgaa gttgccgttg ccgttcaaac agacggggat gtcgattcat
    10741 aaggacatgg atgcgctggc ggatgcggcg gttcagcaag agacgccgca acagctctta
    10801 caacggctat cgagcatgac ggcccgctgt acggcttgtc atgatttgta tcgtttttcc
    10861 ctcgagcgtt gatggcagcc attctccatg gtgtatgagt ccaataaacc acgggggact
    10921 catacagagc agagatccga agcatactcg tcttcaggag ttgcaaagga ggacacaatg
    10981 gcacgcgtag tgatcatcgg agcgtcgatc gggggtctgc ctgccgccta tgaggcgcag
    11041 gccttgctgg gaaagaaaca taaggtgacg gtgatctcga acgtggactc attccacttc
    11101 gtgccatcga acccgtgggt ggcggttggt tggcgtaagc ggaaggatat cagctttgcc
    11161 ctgggacccg tcctggagaa gaaaggcatc gaattcatcc atgcgaccgc agatcggatc
    11221 gaaccggaac agaaccgggt cgtcacggcg aagggcgagg ttccgtacga ttacttgatt
    11281 gttgccaccg gcccgaaact gaatttcggc gcggtacccg ggcttggccc aagcggctat
    11341 acacagtcgg tttgtaacgt cgaccacgct gaacaggcgt ggggagccta tcagagtttt
    11401 ctgagagatc ctggccccat cgtggttggc gctgcgcaag gcgcttcctg tttcgggcca
    11461 gcctacgaga tggcctttat cctcgatgcc gatttgcgca agaagaaact gagaaagaag
    11521 gtgccgatct attttgtgac accagagcca tacattgggc atatgggact cgccggagtc
    11581 ggcaagtccc gcaggttgat ggaggacgaa tttgccgaac actccatcaa gcccatctac
    11641 aataccgcga tcaaggaggt gcagccgggg aagatgattc tggaggacgg ccaggaagtg
    11701 cccttccgct attcgatgat tatcccgccg tttgccggcg tggatgcagt cgcgggaacg
    11761 ccggggcttt gcaatccgaa aggcttcgtc aacatcgatg cctatcaggc caatccaaaa
    11821 tataagaaca tctatgcggt aggagtttgc gtggcaattc caccggtcga acagacgccg
    11881 gttccgaccg gcgccccgaa gacgggctac atgattgaat cgatggtgtc ggcatctgtt
    11941 cacaacatca aggccgacat cgagaacaac gacaagaagg atacggcgac gtggaatgcg
    12001 atttgtctcg ccgatatggg ggatacgggg atggcgtttg tcgcacttcc gcagatggcg
    12061 ccacggaatg tcacctgggc gaagaaaggc aagtgggtgc atctcgcgaa gattgcgctc
    12121 gagaaatact ttctgatgaa aatgaagcgt ggcgtcagcg agccgttctt cgagaaggcg
    12181 attctcaagg cgatgggtat caacaagctt gaaaaggctg gctaagttga taggccctcc
    12241 gccgcgaatc tttttgctcc gcctctgcct cccataacca agaagaaggg aggggaatag
    12301 atgaaactca acgaactctt gcggttgata gcgggggtgt ttgtgctgct tgccgtcttt
    12361 ctgggtgcga cggtccatcc ctattggaat tatttcgctg catttgtggg cctcaacctc
    12421 atacagtcgg cctttaccgg ctggtgtccg atgatggccc tcttgcgtaa gctaggcgtc
    12481 caggagtagc ggtggcggct cggtcactcc cctataccgg tggaatgcgt acactcagaa
    12541 ggacctcgtt gggcgcgcac agtcggggac tcatgcacgt gacgattgct ccagcgacac
    12601 gatggctcat tgttgcgtgc cttttactgt cggccgggtg cgggtccaag gaggaaccga
    12661 caacgtccgg cctgtcggcc gcgccgcgcg caactattca agcagcggta gtggagatca
    12721 agactgtccg ggttcccgtc cgagtggaag tgactggtca agtcgcgact gtctatcagg
    12781 cgactttatc cagccgcatc caagggacga ttgataagct gcttgtcaga gaaggcaccc
    12841 aggtttctaa agggcaaacg ctcgttcagt tggatagtcg cgatgtccag gcggacctgg
    12901 cacgcgcctc agcggaggtc gagaatgcga gagcgcattt caaccggatg aaggccttgc
    12961 tggatgatca ggccgtctca acccaagaag ttgaaaacgc cactcgtgca ctcaaggtgg
    13021 cggaggcgaa tcgaaaggcc gtattggctc aactcagcta cacggtggtg aaagcgccct
    13081 ttgacggggt cattaccgaa aagaaagtgg aagcagggga actggcctcc cctgggcagc
    13141 cgctgctcaa gatggaggat cctcggcaac tgcggttaga agcaacggtg gcggaaggcg
    13201 acctcaaagc catttcgcga ggggataaga tctcagtggt catcgacgcg ctgggggcac
    13261 aggccttgcg cggaatcgtc agtcagattc ttccggccgg cgatccccaa acccacacct
    13321 tcatggtcaa ggtggatttg ccgagcactc caggactcaa gaccggcatg ttcggccggt
    13381 ttcagctcga taaaggaacg gctcagacca ttcttgttcc ttcttcggcc atcgttgagc
    13441 gcggcgaact gaccagcttg ttcgttgtcg gcgcggacca ggttgcccga ctccgctggg
    13501 tcaaggtcgg acgacgtttc gagaagcaga tcgaaattct gtcaggcgtg aacgtggacg
    13561 aacgcgtgtt agtggacggc agccgcggtg tcgacggcgc tccggtgcag atcgtcaaca
    13621 ccgttgcagt accagccgtt ccctagcgac ctatcatgga tcagtatcgc ccagggttga
    13681 gtggtcggat tgccgccctg ttcatcggca gcaagctgac gccgctcatc atgttgggca
    13741 cattgctgct cggactgttt gcgatcgtcg tcactccgcg cgaagaagaa ccccaaatca
    13801 tcgttccgat ggccgatgtg tggctgccct ttcccggcgc gtctgccaag attgtcgaag
    13861 agcaactgac caagccgttc gagcgcaagt tttccgaaat caagggcgtg gagtacgtgt
    13921 actccatttc acggcctggc ggcgcgttga tcatcgtacg gtatttcgta ggccagccga
    13981 tggagcagag cctggtcgat ctctacgata agttgatgtc gaaccaggac ctgctgccgc
    14041 ctggggccga gacctttctg gtcaagccga aggatgtgaa cgatgtcccg atcgtgacgg
    14101 tgacgctgtc gagccaacgg tacggcgaat atgacctgta tcagttggcc gaacaggtct
    14161 tggaggaggc caagaaagtt ccggggacgt cagccgggtt catcgtcagc ggtcaggcgc
    14221 gggaactacg ggttcggatt gatcccgcca ggttgaaggc ttacgggctc acgccgctgc
    14281 aaatcgcggg ggtaattcgt gcagagaatc gagcgttgcc gacagggcgg ttcgacagcc
    14341 gcaatcagag ttatctcgtc gaaacaggcc ggttcattcg atcacgagaa gatctggaag
    14401 cgctggtcgt cggcgtgcac gaacagcggc cggtctatct ccggcaagtc gccgatgtca
    14461 ccgatgggcc gcgcgaatcg aaccagtatg tctggttcgg gctagggccc ggagacgcca
    14521 gtgaaggggc aggggcaagg ggtgagggga aggcctccga ggccgcgccc gatacttcac
    14581 gcctgatgcc tcacgagagt ccggcggtga ccgtagccat tgccaaacaa ggcggtacca
    14641 acgcggtgac ggtggcaagc gaggtaatcc ggaaagtcga gggtatgaag ggtgtgacga
    14701 ttccggcaga tgttcgtgtt acggtgacgc gagactatgg agagacggcg caggagaagg
    14761 ccaacgaatt gctgtggcat ctgctcatcg ccgtcgtcgc ggtggtgggt tttctcggcg
    14821 tcgcgttggg gctgcgcccg gccatcgtgg tctcgctcgc gatcccgctc acgctggcct
    14881 taaccctctt cacatcgatg ctgatcggat acacgatcaa tcgggtgacg ctctttgctc
    14941 tcatcttctc catcggaatt ttggtggacg atgcgatcgt cgtcgtcgag aacacatatc
    15001 ggcatttgac gctacacgtg aaaccccatc acgaggcctc gatcgttgcg gtggatgaag
    15061 tcgggaatcc gacgatctta gctaccctta cggttattgc cgcgctattg ccgatggcct
    15121 tcgtttccgg gttgatgggg ccctatatga ggccgattcc cgtcaatgcc tccattgcga
    15181 tgttcttttc gctcctggtc tcgttcatcg tgatcccctg gttttgccag acttgttatc
    15241 gaactctggg cgggaactca gcactcagca ctcagcactc tgaccttgac gagcgtggtc
    15301 tgatggcgcg ggtctatcga cgagccgtct cgccgctgct ggctcatccc atccttgctt
    15361 ccgcattctt tgggatcgtc gtgctgttgc tcataggatc ggctgcattg ttctacactc
    15421 gccatgtcgt ggtgaaaatg ctcccgttcg ataacaagag cgaattgcaa ctggttgtgg
    15481 atatgccgga aggcacgacg ctggaagaaa cggcgcgagt gacgcaggcg ctgggacggt
    15541 atgtgaggac tctgcccgaa gtgcgggact atcaggcgta tgtcggcaca gcctctccgt
    15601 tcaactttaa cggattggtg cgccactatt atttgcggga acagccccat gaggccgata
    15661 tccaaatcaa tctggtccca aaacacgaac gagctgccca gagtcacgaa atggcacagc
    15721 ggattcgtcc tcaggtccag gagattgcca agcagtacag ggccaatgtg aaagttgtcg
    15781 aagtaccgcc tggccctcct gtgcaatcgg tattggtggg ggaaatctac ggcccggatt
    15841 acgggcggca gatcgccgtc gcgcgcgagg ttcgagcgtt atttgaatcc actcccggtg
    15901 tcgtcgatgt cgacgattac attgaagccg atcaggtgaa atacatcttc accgtcgatc
    15961 gggcgaaggc cgcactggca ggcattccga ccgaggacgt ggtgaagacc ctgcgtatgg
    16021 cgctggaggg cacgaaaatc gggctggtcc atattccaga ggaaaaaagt cctgtgcaga
    16081 tcgtgctacg ccttccgctt gcagagcgaa ccggacttgg gagcatcggc gagatcggtc
    16141 tgcggacaaa tacaggcggt atcgtgcaac tgtccgaact cctcacagtc gaacagacga
    16201 tccaggacaa ggcgatttac cacaagaatc agaagccggt ggtctacatc gttgccgacg
    16261 tcggcgggcc cggtgcggag aaagcggaga gtcctgtcta tggcgtgtta ggtgttggga
    16321 aaaagctgga aggtttccgc ccggccgagg ggtatcagat cgagcaatac tacgcctcgc
    16381 agccctggtc ggaagacaag tttggcatga agtgggatgg ggaatggcac attacctatg
    16441 agacgttccg cgacatggga attgcctttg ctgtggcgat gctgttgatc tatctgctga
    16501 tcgtcgggca atttcaatcg ttcctcacac cgttgatcat catggctcca atcccgctca
    16561 cgctcatcgg tatcctgcca ggtcattggc tgaccggttc ctactttacc gccacctcga
    16621 tgatcgggtt cattgccctg gccggcatca tcgttcggaa ctcgatcctt cttgtggatt
    16681 tcattcagtt gcaagagcaa gcgggcgttt cattatcgga tgctgtcatc aaagccggcg
    16741 ctatccgcac aagaccgatt ctcttgactg ctgcggcgct catggtcggc gccttcgtca
    16801 tcatcctgga tcccatcttt caaggcctgg ccgtgtcgtt gctcttcggt gtcggcgtct
    16861 ccacgctgct gacgttgatc gtcattcctg tggcctacta tcacgtgatg gggagaagga
    16921 cggcctcctg accgctgcgt gatcccttca cgggatcata aattccacgc agcgttaaat
    16981 ccggaagctg agaagtacgg ttactgaatt gtttcacttc aaccgaatat attgatgaag
    17041 catcgccttc ctatacaaca tgttgtagct tggaaaccct taagtaatag actaggattc
    17101 gggctcgttc ctccgaccct cttgagagtg tttgatgccg aatcaaacga ccgaacaact
    17161 ttcattcccc gcaactccgt ccgcagcctc ttgcctccac agccgtgtca tcgatgatgt
    17221 gttgaccaaa gacgggaagc gcacgggcca ggttcgctgt ctcgaatgtc gcgaagtttt
    17281 cgaggacccc gatcaagagt ccaaatgact caggcgacta tgcccgggaa ataaaatcgc
    17341 gccctggtgc gttctgcgac agcgaagtat atctccctgt agcaagactc ccctatccgg
    17401 tagcacgtag ccctcttagt ttcatcaaac aagtcagttt tcctgccgaa atgtcccgat
    17461 ggcgagactc cggaccatgg ttccctcctt gcacttctgc cgttgtcagc tggcaggaga
    17521 ttgctctacc tgacgcgaaa aatggatggt tcggcgattc cgtagtgcca tgattagcag
    17581 ggtcctcatc gctgaaatgg tcctctgcgc cccactcgat gaaacgttgg ctgcaaagga
    17641 agccgggacc tccgctccgg agtgacggct gagtttgcgg gatgcaattc atgcggcgat
    17701 cgagaaccat gtccgcctct tgaaagagcg gattgctgcg gcataagctc aggccgagac
    17761 cagtctgggt gcgctgctgc cgaatgtatc cagttagctg aacggcagag atcgcacggt
    17821 gaatttcgcc gccttgggag ttcctcaaga taggcttggt cgtccagggc taaggggtgg
    17881 tgtcaccgat cccttcgatc tctatgacgc gcgggccggt ctggcggtga gcatcgaagt
    17941 tacgaacgcg cagacatcgg tgacacgcgc ttgcgagaat cagggtgaag cgttgttccg
    18001 attcaatgct tcgcccatca atccacccgg gcgaaggggc aaatcgaaaa gatcttttga
    18061 gcggaccgaa tcaatgatgc atcccctctg ttgtttgaga tgaggaatcc caagcgcgat
    18121 gccctcatag tcacggggtt ggtcgccttg cttggcgggg gctacctggt cgcgtggacc
    18181 ggtttttgga accgcggatt accggaaggc ttgattcagg tcaacggccg catggagggc
    18241 gatcacctga ccattgctag taagtttccc gggcgtattc aagaactgct tgttcgcgag
    18301 ggggacaatg tcacgacggg tcaaatcatg gttcgaattg atgacgtcca gacccgcgcc
    18361 agggttgatc agacccagca agccttcgct gcgatcgagg cgcaagtaca ggcagcgcat
    18421 acgactctgg ctgttctcaa tctggaggtg cccttagcga tcgagtcagc ccagtctcac
    18481 gtcgatgaag atcgggcgga gttggagaag gcgaagacgg tcgagcggga agcccgcatg
    18541 gacgagcagc gtttccgcaa cctgcttccc gaacaagcgg tgacgcaaca acagtatgag
    18601 caagcgcttg ctcgatggaa cgtggcgagg agccaagtgg tggccgcacg gtcggcactc
    18661 gcgaaagcga cgaaggaact tgcggtagcg gaattggggt ggaaacggat tcgcgccaag
    18721 gaggatgagg tcgccgcgct ggagcggcag cgggaccagg ccgaagcggc tttgagtgaa
    18781 acagagagcg ttctggccga tctgacgatt cgtgcgccgg cgaacggaac cataacgaca
    18841 cgaatggtgg atattggtga agtcgtcacc gccggggcgc cactttttga ggtcgtggat
    18901 ctggatcgcc tgtatctcaa agtctatgtg ccggagatcc aaatcggaaa aattcgattg
    18961 aatctgcagg cccgaatcta caccgatgct tttcccgatc ggccgttcga tgcgacggtg
    19021 cgatacatcg cctcaacagc cgagttcaca ccgaaagagg tccaaacccc ggatgagcgc
    19081 gtgaagctca tctatgccgt gaagctctat gtgaaagaga atccggatca tcgactgacc
    19141 cccggcctgc cggctgatgc agtgattcgc tggaaggaag atgttgcatg ggtgccgccc
    19201 agacgatgat gagggaagcc atcagccatc agctaattcc gaaactgata gctgaatgct
    19261 gatagttgac agttcgtaag ccatatgtgt ccaacgaatg aactaatcat gagccgagac
    19321 tctacaatcg ttcatgtggc gcaattcgtg aagcgttaca aaaaacatgt ggccgtgaat
    19381 ggtgtggatc tcacgatcaa gaaaggtgag atctacggcc tgatcggtcc ggacggcgct
    19441 ggcaagagca gtctgatgaa ggcggtcgcc ggtgtgctga gctacgacgg cggtgcagtg
    19501 gaggtgttcg ggacactggt cgattctgag cgtgccgctg agcaggtgaa acagcgactc
    19561 ggtttcctgc cgcaagggct gggactgaat ttgtaccctg acctttcagt cgaggagaat
    19621 atcgacttct ttgcacggct caggttggtc cctgatcgcg agttggccga gcggaaggac
    19681 cggcttcttg ccatgacgag gctcgacaaa tttcgtgatc gggccatgaa gaatctgtcc
    19741 ggcggaatga aacagaaact cgggctgatc tgcaccctca ttcacgaacc ggaactggcg
    19801 attttggacg aaccgacgac cggggtggat ccggtgtccc gccgggattt ttgggcgatc
    19861 ctggcagagt tgctgcaaga gaagggcatg accgccctcg tttcgaccgc ctacatggac
    19921 gaagccgcgc gattccatca cttgtcgttt ctctctcacg gaagggtctt gacggcaggg
    19981 actccggcgg aagtgcaggc gctggtcccc gggtcgatga tgacgttcga agccactcct
    20041 caattggaag ccgtcgcccg gctgcagcgc aggtacaagc aggtggaggc actgggttcc
    20101 aagctgcatg tgttcacgga cgagacggac cgtgctcaag cggtgaagag tctcgagacg
    20161 aacctcggcg acatcaagcc ggaacaggtg cgtgtgggcg agccggaact ggaggatgtg
    20221 tttgtcgcgc tgttgctccg gcaggccacg ggaccggagg ttcccgcctt gccggcgact
    20281 ccggggcagg cgtcgaactc agggcttgcc attgaagcaa gggaactcat tcgggatttt
    20341 ggaacatttc gggcggtgga tcgcgttagt tttcgggtca agccgggaga aatcttcggc
    20401 ctgctcggtg caaacggtgc gggaaaaacg accgtgatca agatgctgaa cggcatcttg
    20461 tccccgtcag gaggggaggg ttgggtagcc ggtgccgata tgcgcaaagc cggaggtgcg
    20521 atcaaggagc ggatcggcta catgtcccag gccttttcgc tgtatctgga cctgaccgtc
    20581 gaggagaaca tccggctctt cgccgggatc tacgggctcg atcggcaagc ggcgaatgag
    20641 aggctcgcat ggatcatgtc gatgggagga ttgaaaggct acgagtcgag cctgacgggt
    20701 cgtctgccga tgggagtccg tcagcggctc gcgttaggct gtgcgttggt ccatcggcca
    20761 cgcgtgctct ttctcgatga accgacttcc ggcgtcgatc cgatcggacg gcggcatttt
    20821 tgggatattc tgtcacgcct ggctcgcgag gagggtgtcg cgattctgat caccacccac
    20881 tatttgagcg aagccgagca ttgcgatcat ctcgcgttga tgtttgccgg tcgaatcgtg
    20941 gcggaagggt ctcctgccgc catgaaggac caggtcgaac atgaagcggg ccacctcctc
    21001 gaagtggcga cggatcaacc gggggcggcg ctccgacgat tgacgcaggc gggattcacc
    21061 gacgccgcgc tctttggaac gaagattcac ctgttctcga ttgatcctgc gcaggacgaa
    21121 gtgcgacttc gggaatctct tgcctccgac ggagttcata tactcgcgat gaatgtacgt
    21181 cctctgagtc tcgaagacgt cttcgtccat cgcgtcatgg cgctcgagcg gcaggagcga
    21241 gaggcaacga cgggagcggt ggcatgaatc tccggcgtat cgcagcggtt tcctccaaag
    21301 agtggcgcga ggtgattcgc gaccggatgt ttgtgctcct tgccttctta atgccggtcg
    21361 tctggatgct cgtattcggc tatggcctcg tcctggacgt ggaaaacatt tcatttgtgg
    21421 tggtggatcg ggaccacagt tcgctcagcc gggaatacct gtaccgcttt gtgcagtcgc
    21481 gctatttcaa ctttcaggga gaactcgctg ccgagagaga tgccgacgtc tggttgcaga
    21541 gcgggaaggc tcgtgcggtg atcatcctgc cggaaaagtt cgaggagcgt ctgttgtccg
    21601 ggcagccggc caatgtccag actctcattg acggctcgtt cccccttcgc gcggacatca
    21661 ccaagggcta catcattgcg atcaacagcg ccttcaatga gggcctcctg gtggatcatt
    21721 tggcacggac ccgtggcctg tcacgcgacg aagcgcagcg ccggctccgc cctctcgccc
    21781 tcgaagtgcg gtatttatac aacgaggaag tgcgcagcgc ctggtcgaca gtgccagcgc
    21841 tcatcatggt ttccctcatg gtttcgtccc ccctgttgac cgcgctcggc atagtccgtg
    21901 agaaggaaac cggttcgatc tataacatct acagcgccac ggtcagccgg ctggagttcc
    21961 tcgccggcaa gctctttcca tacgtcatga tctccgcggt caatgctatg gtgttgtggc
    22021 tgatggccgt gttcctgttt gccgtgccgt ttaaagggaa cctgctgttg ttcttcgcca
    22081 cgtctcttct gttcgtattg accagcaccg gtatcggcct ggtggtctct ttgctggtca
    22141 gcacgcagca ggctgccctg atcattacca tattgctgag cacggtaccc accattctct
    22201 tctccgggct ggtcgtgccg gtctcgtccc tcagccaagc ggcgcagttc caggcgcact
    22261 tgtttccggg gatgtattac acaaatatcg tcaggggcac gttcctgaag aatatcggtc
    22321 cggcagtctt atggccggac attctcgccc tcggagccta tgcagcggtg cttggaacca
    22381 tcggctatcg attgttcacc aagaggccgc gcgcatgaaa ccggcagctc gcatcgccgt
    22441 ctggtgccga cgtgtcgccg tcatgacacg gaaagagttg atgcagttgt tccgtgacat
    22501 cccgctcatc gcatttctcg tctattcgtt cacattcgcg atcttcgtca ccggaaacgg
    22561 gatcaagacg caactgagaa atgccggcct ggtggtctac gatgccgatc gaagcgtgtc
    22621 atcccgcgaa ctggtctccc gatttcatct tccttacttt cgtttcgacg gcgagttggc
    22681 gcatccggac gaagaattgc gtcggctgga tcggggaagt tcgatggtgg tgttggacat
    22741 tcctgcccgc tttcatgagg cgctggcagc aggggaaccg accgccgtgc aattgcttgt
    22801 cgacacgacg aactcgccgc aagggctctc ggcggccgga tacgccgccc gcatcgtggg
    22861 tcaattcggc caggaagtgg cattggcgcg aaatgggctg gcggacgcgt atttaacaag
    22921 cgtgccgatg atcctgagcg accatcgcgt ctggttcaac caagaccaga atgaaacgtg
    22981 gttcgaatcc atttcgcacc tgttgcggat gatcacgatt ttttccgtgt tgttgccggc
    23041 agcggcactg gtgcgggaga aagagcgcgg cacggtggag caacttctgg tatcgccgtt
    23101 gaccccggtg caaatcatcc tccccaaagt cgtggcaatg atgttggtga ttctgtgtgc
    23161 gacggcggtg ggtttgtttg gcgttatgcg gccggcgttc ggtgtgccga tcaaagggag
    23221 catcgggctg ctgttctcct tgacggctct cttcatcatg gctacggccg ggatgggggt
    23281 gttcgccgcg accgtcacca ggaatcaggc gcaggtgggg atgttgacgt tgctcgcggt
    23341 cgctcccatg ctgttgctct ccggcatctt cacgccgctg gagatgatgc ccgcatgggt
    23401 acgtcatctg atggcattct ccccgctccg ctatttcatc gaaatcgtga acggcatcct
    23461 gttgaaaggg ataggaatcg agatgttgtg gacgcagacc ctttcaatgg gactggtggg
    23521 cggatccttg ttcggctttg gcctgtggcg gtttcggagg caatttcagt gaaggagagg
    23581 cagaagagca gggtgacagg ctcgtctacc atgacggttt caaccagtag gggcgcttgt
    23641 tcgcgcggac aagcgcagca gtcggagtcg gactgtgcgc gggcttcgtc atgtacggcg
    23701 gcatgaaagc cagccggcct gaacttggga cggttggtcg aactccgccg accccggttc
    23761 aagaggggga gacatgaata agaaggtctc gttttccatc tggtacatca tcctcgccat
    23821 ctgggcggtc atcctggtcc atgacttcat tagtgcggtg cagaagatcg aagagttccc
    23881 ttatagcgag ttcaaaacgt tggtggcggc cggaaaagtc gcagaagtgt cggtgagcag
    23941 ccacatgctg acggggaagc tgaagccgga aggagagtcc aaagagcaga aacagttcac
    24001 atcaattcgt gtggaggatc cggagcttgt gcgggacctc gaacaacatg gcgtgaaatt
    24061 tacaggagtg atcgagacga cgttctggcg cgacctctta tcctggcttc tgccggttct
    24121 tgtcttcgtg ggcatctggt tcttcatctt ccgtcgactg ggccaggctc aaggcgggtt
    24181 catgcaggtg ggacaatcca aggccaagat ttacatggag aaggacatca agatcacgtt
    24241 tgccgatgtc gcaggggtag atgaagcgaa agaagaactg cgcgaggtga ttgagttcct
    24301 caagactccg gacaagttca ccaagctggg tgggaagatt ccgaagggga tcttgttggt
    24361 cggcccgccg ggaaccggca agactcttct ggcccgtgcc gtggcgggcg aggccggggt
    24421 gacgttcttc agcatcagcg ggtcggagtt tgtcgaaatg ttcgtggggg ttggcgcggc
    24481 gcgcgtccgc gatctatttg agcaggccaa gctcaaggcc ccttgcatca tctttatcga
    24541 tgagctggac gcgctgggga aagctcgcgg catgggcccc atggcgcacg aggaacgaga
    24601 gcaaacgctc aatcagttgc tcgtcgagat ggacgggttc gatccccgaa tcggcgtgat
    24661 tctcatggcg gcgaccaacc ggccggaaat tctcgacccg gcactccttc gagcgggccg
    24721 gttcgatcgg cacgtcctgg tcgatcggcc ggacaaaatc ggccgtctgg ccattctccg
    24781 ggtgcatgcc aaacaggtgg cgcttggtcc cgacgcggac ctcgaaaaca tcgcggcgat
    24841 gacgcccggc ttttccggag cagatctggc gaacattatc aacgaagcgg cattgctcgc
    24901 cgtgcgtcgc ggcaaagacc aggtcgggat ttctgaattg cgggaagcgg tagagcgggt
    24961 gattgcgggc ttggaaaaga aaaatcgggt gctgaacaag atggagaagg agcgcgtggc
    25021 ctaccatgaa accggccatg ccctcgtggc gatgtccctg ccaggatccg atcaggtgca
    25081 gaagatctcg atcattccac gcggcattgc cgcactcggg tatacgcttc agctcccgac
    25141 cgaagatcgc ttcttaatga cgaaatccga gttggagaac aaggtcgccg tgttgcttgg
    25201 cgggcggatt gccgaggaat tgatcttcgg cgaggcttcc acaggcgcgc agaacgattt
    25261 ggtgaaggcc accgatattg cgaaaagcat ggtgaaagcg tatgggatga gcgagaagct
    25321 ggggacgatc acgctggaac gcgagcgcca gccgcagttc gtccagattc agatggccca
    25381 ggaaaagggt gattactccg aagaaaccgc ccgtgagatc gattgcgaag tgcggcggat
    25441 cgtcgatgaa caatacgaac gggtaaacaa gttgcttgga gaaaggaaag ccgcgcttca
    25501 agaaggcgcg aagctgctgt tggaacgtga agtcatcagc ggggtggagt tgaaagcgat
    25561 catggagaag acctgaaggc gtcagccgca atggtctata
//
LOCUS       DATIFR010000119        51581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_845405, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000119 DATIFR010000000
VERSION     DATIFR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 51581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51581
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845405"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VLD60_06870"
     CDS             <1..1266
                     /locus_tag="VLD60_06870"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSB44714.1"
                     /translation="YRARWAGFGWHALVIDGHDLSAILAAFDEASRTKNTPTVLLAKT
                     FKGRGVSFMEDHPSWHGKPVPQGADTQKAIDELTKQLKPNGATLTIQKPSAASVAASL
                     VSPLPASPYKPGESAATREAFGVALEALGAVNPSVVGLDADVKNSTYTDKFGKKFPGR
                     FFENFIAEQNMLGAAAGLAACGKIPFAATFAAFFTRAYDFIRMAAISQSNIKLVGTHV
                     GVSIGEDGPSQMGLEDIAMMATQPGITVLYPSDATCMYRLVEAAANHKGMVYLRAGRP
                     KSPVLYGPAERFQIGGSKILRQSAADALTIVAAGVTLFEALKAHDQLKAAGISTRIVD
                     LYSVVPIDRVTLLDSARATHGRVLTVEDHYAHGGLGDAVLSAVGAEGIKVHKLAVRTI
                     PHSGKPDELVDHFGIGARSIVEAAQQIIK"
     gene            1235..1963
                     /locus_tag="VLD60_06875"
     CDS             1235..1963
                     /locus_tag="VLD60_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSB44715.1"
                     /translation="MSKQRNKSSSNRAGAVPQGQLAQIPLLNILSGQDRQRLLHELTE
                     ARYGKGEFIFREGDPTEYFHIVKEGTVKCVKSNPDGKECTLKMLMPGDLFCCDAAAFD
                     GARHPGSAQPMGDVSILRMSKKAYFDMLRRSPDAALEVIKYLGNRLNEAQEKTKVLAL
                     ERADQRLAALLVDLGTRSGIKEDQGIRLPMRLTREDMANMVGTTTETVIRMMSRFKQD
                     ALVTGTANRLIIRDIERLKKLAST"
     gene            2151..3140
                     /locus_tag="VLD60_06880"
     CDS             2151..3140
                     /locus_tag="VLD60_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSB44716.1"
                     /translation="METIRSHRSLALAAGLGTFMLAGLMAPATPASAKTHEVSMTAVE
                     TDVVIDGSGEKYAAWTFNGTMPGPVVRVMEGDTVKFTLTNPATNKYPHAMDFHAAEID
                     FLKNYRAINAGETITYTFVAKKPGIFFYHCGAPPMIQHVARGMFGAIIVDPKDAKALP
                     KADREYVLVQSEYFKNPDDVQGMFDRKFDGMMFNGGIFKYHPFVTGGGKLDAKPGERV
                     RIYFVNAGPNEFSSFHPIGEIWDNVYESGNPANKLTGVQTYVVGPGSAATFDVVVESA
                     GAYPLVTHSLTGALRGAIAVLLASPDAKPAPLMPMVPWKPTTPQTEITPVPAQ"
     gene            4577..5446
                     /locus_tag="VLD60_06885"
     CDS             4577..5446
                     /locus_tag="VLD60_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HSB44717.1"
                     /translation="MDYVKPQEIVSDMVSTGALKLDLPVRHLLIRGALAGAYLGVATS
                     MAVTAAVETGSWLVGSLLFPFGLCLAVLLRTEIITGSFALLPCATADGKANACVRRVL
                     INWGWVFLGNLIGSTLYAGLLAIVLTTAGDAGVSAAGAKLIAIAEAKTNYYASHGSAG
                     MLTVFTKGILCNWMVSLAVVFAFATTSLSGKILAIWGPTVLFFSQGFEHAVVNMFVIP
                     VGMLLGAHVTVTDWWFWNQIPVTLGNLVGGMIFTGLAIYFTHRPTPAVSEARVSAVPD
                     NAHEKRPRYSPSF"
     gene            5472..7064
                     /locus_tag="VLD60_06890"
     CDS             5472..7064
                     /locus_tag="VLD60_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HSB44718.1"
                     /translation="MSESHSTRLAGWQFFLFNLVLGIAHMLVLFNAGSYVALLPHAAG
                     NLGGVLPSFGTWAQTDFMIALALAFPIARWLSSRFGEVRVFIGAFVLYAGASLLCLIG
                     DTITTFLPARILLGLAGGLTLPLGQTLLLKEYPDRLRSLGLAVWGLFTLMPFTLGLAT
                     GGWIADELGWRALFALNIPFALAIAGVTAALLYRRTFVPHRERFDLVGFVLLTVIMIG
                     IQTILNEGNDFDWFDSALLSGMLVVVLVAIPVWIIWELGERQPAVDVRLFTHRNFVIG
                     LLCLALGFLSIQGLLALFVVQLQTLMGYSSELAGMVFLPMIILGVPLIAVMHEVAKRL
                     DVRLLACLNGLGFAATFYWIGLFDDPQSFDQIFWPMVLEGVFLGSFFTPLTVLTLHGL
                     SGDQMLRAAEAANVFRIAAGAVGITWQGVVVFRRLPFHQLQLSEHFGGRMSASYDPLQ
                     QITLKLQDTGLDPVMIQRKLQLLMKQEAGILALNDAFLLSSYLCIGLAVLVWFAQASR
                     VPVLKPVEAVRELQAEELMEQP"
     gene            7061..8569
                     /locus_tag="VLD60_06895"
     CDS             7061..8569
                     /locus_tag="VLD60_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSB44719.1"
                     /translation="MIRTRSRLNGLFLNGLVAGALVMASGCAWIPKGDPPAEYLEPPE
                     MKETLAEVTSRLQQWPDDQWWKQFGNQELNELIDTALKDNPGFKVASARLREAEGLVK
                     VEGARLLPFLEADASLTYERISQHGVFAALNPKVAGDKILLGVINPLSFRYEFDFWGK
                     NRATLEAALGLAAAEEAERAEVRLRLTTGIARAYFRGQALQQQLTVVKTIVAIRENLR
                     TLAETRYRLGLDNDQPVKVAVADYEAAFKRQAGLQDQLDVQRHLLARLAGKGPDQAAH
                     LFLKPVVIPGQIAAPDHLSMGLLVHRPDLAAALYRAESAARMVKVAKTQFYPTIDLTA
                     FVGFNALTLGKHADKLSNLLFSGQSFAYGFAPGLRMPWFEGGRLRGELSAHRAEYDAA
                     VELYNDTLLDAMREVADSLSAWQSTRKMIESHRRLLASLGEDWRLAKVRLVTGLDDDR
                     EVLRHRQPVLEQEYALRALESDQLVAAIDLIEALGGGYHNPDVEKRPAQKPS"
     gene            8573..10081
                     /locus_tag="VLD60_06900"
     CDS             8573..10081
                     /locus_tag="VLD60_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSB44720.1"
                     /translation="MSTTHSIQPPAPDLYADSLRSQRNRRLVIVILALGLAGLAYGAY
                     WWTTARFWVETDNAYVTGNLVPVAAQASGIITQVLAEETQFVNRGDILLQLDQHEAYA
                     ALGRSRGRLGETVRRINALFVTKRQVEEKLAARRARLDIVRHDVDRYRLASPSGAVSK
                     QILQNATDQLQALEADVRETQAEYDALDAQIGGTSVMEHPAVDLAKHEFILAHLEYAR
                     QQIRAPVSGYVAKRKAQVGDRVKPGTNLMTIVPLDHLWVEANLRETQLAEIRPGQTAE
                     VQVDLYGSKHTFHGTVEGLVPGTGSPFALLPPDNSTGNFIHITERVPVRIALASDEIR
                     EFPVRPGLSTVTRIHVSERGNSVWSSLAKPDTEEYQTDVYGDELTSAESMAQGVIAGN
                     VVVNAEFSRERSAATLYPVALIDQDPEISSYSPEHKDDRSHRTSRQRTQEMTPESYVP
                     PRSPDLGSAYFPLSPSIGPGSSRLGPAAGPDASRRKFLGETETRTNGIGTRR"
     gene            complement(10094..10411)
                     /locus_tag="VLD60_06905"
     CDS             complement(10094..10411)
                     /locus_tag="VLD60_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB44721.1"
                     /translation="MQVIKLNDYQSFSSEKMKKNNIFETVRFFCDVYCFEPGQEQKGH
                     IHGEQDKVYLVLEGQGTFQVGSEKQVLGPGQGTMAPAGEEHGVKNHTSQRLKVLVFVA
                     PNP"
     gene            10553..12241
                     /locus_tag="VLD60_06910"
     CDS             10553..12241
                     /locus_tag="VLD60_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44722.1"
                     /translation="MNRSERRRQEAQKGTSHFSGQVTTLLSEGKTYHQAGRLVEAETA
                     YRQALALAPHHPEALHLLGLALYRGNRLEEAINCIKAAIEQQPSSPLYWFNLGVILQK
                     ASRQEEALTAYRKAVALNPRYLEAYINLGNALRDRGDLTESIEVYEKALTLNPSNAET
                     HNNLGVALKEQFRTDQAIASYRRALELKPTHVEALNNLGLALMETGTLQEAITSFQRA
                     LAIMPGYQKALYNLGIAWSWAGDDGKSVDCLQQVAKAKYDHGKIVTESLIYRSRLKHD
                     IEQTQYLLDQHKLGNEYRPYLQSLRELQEHLNRTPSTRNRTTIEPNRLAPIAPSFNRV
                     LYCVPPPRIEHGALNPSLDAASVETRYLEKHPEVTFIDGLLNQEALNGLRRFCWEATI
                     WKKDYENGYSGAFLGDGFASPLLLQIAEELRLKFPRIFTYHRLTQAWAFKHDSARRGL
                     NIHADAAAVNVNFWITPDDANLNPATGGLVVYDKEAPKDWNFKAYNSDQNKPKILAWL
                     EQVGAKAIKIPYRANRAVVFNSDLFHETDEIAFKDDYLSRRINITLLYGYRISA"
     gene            complement(12280..12636)
                     /locus_tag="VLD60_06915"
     CDS             complement(12280..12636)
                     /locus_tag="VLD60_06915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB44723.1"
                     /translation="MEQRKTPRYPVRFRSSFTSLNIVGGDGRIVDLSLRGCRVDSSTE
                     VRPGTSLEVRIQTSEEEPPLKIQEGVVRWSRGQQFGLEFVTLVPEEWARLQHTVTQLE
                     LHPYQTQVDPDGSEAA"
     gene            complement(12663..14114)
                     /locus_tag="VLD60_06920"
     CDS             complement(12663..14114)
                     /locus_tag="VLD60_06920"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSB44724.1"
                     /translation="MKLNTDMKVNRITNEIWEIPTSEKSGMLVPARIYATPTILQSMD
                     RGVFDQVTNVACLPGIQRYALCMPDGHWGYGFPIGGVAAFDVQDGVISPGGVGYDVNC
                     GMRLIRTDLTLDDVQPRLERLMTELFRRVPAGVGAGGFVRLDREAFDRVMTKGARWCI
                     EEGYGWHRDLVHIEEQGCMGGADPSKVTDHAVGRGINQLGTLGSGNHYLEVQVVSNDR
                     IFDPVTAAALGIIGQDQIVVMVHCGSRGFGHQVASDYLKVFEKAMRRYGIAVKDQQLA
                     CAPFRSPEGQDYFAAMNCAANTAFANRQVITHQIREAFATVFGKSAEELGMELVYDVA
                     HNIAKVERYSEGELVVHRKGATRAFGPASPELPQDFKKIGQPVICGGSMETGSYLLVG
                     TDQALRDTFGSTMHGSGRTMSRAQAKKSVRGEQLQQQMKQRGILVKAVSMSGLAEEAG
                     FAYKNISEVVEAVDRAGITKKVAELRPIGNIKG"
     gene            complement(14111..14545)
                     /locus_tag="VLD60_06925"
     CDS             complement(14111..14545)
                     /locus_tag="VLD60_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSB44725.1"
                     /translation="MAFTYRYLDDIALADVAFEVLGDSLPDVFAGATQAILETLANPA
                     TVGASWERAITLLDQDLSNLLFEWLSEIVYWKDAAGVVFREAPLTVSLTSGIWTLQAR
                     LVGAPVDQQRQELRNDVKGITKHLYELKQDGNQWKATVVLDV"
     gene            complement(14602..15747)
                     /locus_tag="VLD60_06930"
     CDS             complement(14602..15747)
                     /locus_tag="VLD60_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSB44726.1"
                     /translation="MTRSASQSHKATVFIAASELDSNLYYATKFIAPDPFIYLEIKGE
                     RLLVMNDLEMDRAKSQAKVDRVLSYSEIERRARDHGVATPGSVDIIHVVLRDAKIKHV
                     LVPANFPFSHASRLQELGYQVHAKPDPFYEQRVVKTAEEVRLIEAAQRATEEAVVAAH
                     DLLRQASIKDDSLWVDGSVLTSERIKKLINVKLMERDCVAQHTIVAGGEQACDPHHEG
                     SGPLPAHRSIIFDVFPRSATSRYFADMSRTVVRGRPSEDLKKLYQIVKDAQEEAITKI
                     KDGADGMKIHRGICERFEKAGYKTGLVNGRMQGYFHGTGHGVGLDIHEAPRISRTGSL
                     LQEGHVVTVEPGLYYPGLGAVRIEDMVLVTRDGCRNLTNFPKMFELG"
     gene            complement(15985..19347)
                     /locus_tag="VLD60_06935"
     CDS             complement(15985..19347)
                     /locus_tag="VLD60_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HSB44727.1"
                     /translation="MSSARVVVALIAILLIGGMAFLWFSPYLTGADYLKDFFLQQLEQ
                     NLGRKIDVRRIKFVLFPRIRLELTDVAIHDRNSDQVLLSAKKLDLVLRLIPLLRKQVV
                     GKRLLIEGPTLTLRRDRAGHWNVLDRANPLPANNKEAVQLLGRIFRIREATLVNGTVI
                     VIDEARPDGVRTLKLETVEAALIIRTERAQADLHFSAGYSGAHGPAAISVSGVIKKAE
                     QQTLAAEDSSRPELALQFDGHVELANLALRDMADLVGPRPVPDLLQGVVNARSSIRVI
                     PGVAGYDMLLSDLSANMEKLTVNGNASLSGLLTPQPTFAVTFASSPVQVDELLAKIPP
                     AWLHPQLPGVMQDRRINGKVEVASATVTGSYAEGPQLSVTGEFRVTEGQALVGEDHIP
                     ARNLSAVVFVEAGRVRVTSLAGLYGAIHMADSKGLISFLEAGPWLEMDISGTMAAMDL
                     LQFLSRTVNSDQLLRVLAASRDVEGSAALTFRLVGPLSPGTGVTFAGGEIRAQHVNLT
                     NSFLPERLTALQGRFVLAEGGTQFDQVTGHLGDLTVQVQGGITGGTGSSFQDFLVRVS
                     GDAIHMTQLLPGKAIPPGVVEGLATGIMALTGSTEAPHARGELVLTESKVTLPGMMEK
                     PVGAPATIVFEGDVPSSKGVTLTRVEVTVPSIRLPIKGKIQLGEKFSIDAALATGTVA
                     LSTVPEWIAKGGFEAGNLELSLDVKGKDRDWRTWKTTGWLALSNGLMNAKGVDGQIQD
                     LYVRLLLARDAAELKRLSFRLLDSDVTLEGTIKNWTAKPTIVAKIDSNQMDIDLLIPK
                     GARSPIRDFLEMLAATSKVNATASIARGHYKHLKFGGLSARVTIQDGVLDVDRLSGQS
                     TNGDIAGRLVVQLPQGQPADGEISVRATGLLVEDMFKLVGVKDGGITGEARVTGTMRG
                     HGRNPHGLFPSLNGKADVLLENGRVFKSQERAIWKIISILNLPAVLQGKVDLEKEGLP
                     YNKITASVNVHNGVFETENLIIDSPIVKITAAGNYDLPTDQVDMVWAVSPFGSYSQFL
                     KTIPLFGRLFAGERKGFATAMFSVKGSIEDPEVTYLPMKSFATGLTGLAQLAFDVLKN
                     TVMLPIDLVTPDENKTVPKDAIPDAEPVPATP"
     gene            19657..20937
                     /locus_tag="VLD60_06940"
     CDS             19657..20937
                     /locus_tag="VLD60_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44728.1"
                     /translation="MIRQLTGITSIRWLILALLFLTSVVTYIDRVNISVTARHMMPAF
                     GFTNQQMGWVFSAFVAGYALFQIPGGWLADRWGARAVLTGALIWWSLCTAFTALVATS
                     PLADVLGLVGALILVRFALGVGEAVALPCFNRAVANWMPADARGVGIGIAIGGIGLGS
                     AMTPPLAAWVMVNWGWQTVFYLSTIVGLIMGLLWWTLSRDNPAQHPWVQGKDRALIET
                     SQVATPTTVPWYALRNTRTIWWLVLSYSCLGYVAYLYLSWFYLYLVNVRGFSVLQGGL
                     YASTPFLAMLIGCPLGGWVTDRLATQHGITAGRAMAGTTGMTLAGCAVAIGGFIDSPV
                     IGIASLSIGAGCLYFAVGAYWASTTDLSKTHAGTLSGIMNMGANIGGAISPSLTPWIA
                     ARWGWPVSLATAGAVALVGALLWLWIKPGEGLKS"
     gene            20981..21502
                     /locus_tag="VLD60_06945"
     CDS             20981..21502
                     /locus_tag="VLD60_06945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB44729.1"
                     /translation="MSLEFKKIDPRVTIMTKFGEIKIRFYPDDAPRHVENFINLIKMG
                     FYNGTTFHRVVPGFLIQGGDPLSKNPDRSLHGTGSPGYFLTPETSDRPHKRGAIGMAK
                     VPRESNSTRDFNDNGSQFFISVNDNSGLDRTYTVFGEVFRGLDVVDKIVAAPRDERDN
                     PLEPITLTMTVKE"
     gene            complement(21633..23021)
                     /locus_tag="VLD60_06950"
     CDS             complement(21633..23021)
                     /locus_tag="VLD60_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB44730.1"
                     /translation="MRAVIFVTDDELAIRSALAKRLSRQGHRVVAYESGEALLEALQR
                     DLPDLVLLDLKMPGMSGLDALKQVRQVAPTAMVIMLTAYGTVQDAVEAMKLGAYDFVI
                     KSVDLEGIEPVLGRALDVLSLRRRLESEHEHQDSQYQLSNLEAHSHVMKQLLEQVREV
                     AENPKSSVMLLGETGTGKEFLARVIHHNGARASSPFVGVNCTAIPKDLFESELFGFER
                     GAFTGANQRKFGLLEKAEGGTLFLDEIGDLDQSMQAKLLRVIQERSFRRLGGTDDIGV
                     DFRLITATNRDLKKEVGRGMFREDLYFRLNVVAFELPPLRKRVEDIVPLSQRAIVRFA
                     QEFGKPIPDFDPEARAMLERYHYPGNIRELQNILERAMIFCQGRTLTPSCLPVELRES
                     AKQTAVAVGTGTDSMLRVEMRLGQQTLVDIERAIIEEVLRLADYNKTVAAKHLGLTRF
                     ALDRRLKKAEDD"
     gene            complement(23025..24950)
                     /locus_tag="VLD60_06955"
     CDS             complement(23025..24950)
                     /locus_tag="VLD60_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44731.1"
                     /translation="MRQHVASLFDNLPIRRKFLFISAIPLAALVLLSVLTYSSVQKFS
                     QDEEHLNNLYLSEKIAAQYMRLVVDLETGFRGYVLTEQDRYLRPYRVAQEGILLIAAD
                     LKHRISGEALQQFNDIQVLVTQLIAEKEELIKDIKLGRKRNALQYIEEGRGRALMVEI
                     RDRMVRFDQLEQQRVGEELAQLSQDRSSTLFVVLGGGVLTFGLIVSALYLIARSIAVP
                     LVSLSKTVGSSPLGLVPAIDVLARKDEIGDLTRVMHQMGLQIRGHLDEMERSESALRH
                     LNEHLAASEAKYRGLVDHAPFGIFTTKGMEVTFSNRYNQALAGFDPDEPMDPARFRDR
                     FHPEDRERVLSEFARAVSDAQPYETVFRFLHEDGAVRKILSRRLPIKNGEDGLPVYVA
                     FNIDITALDDLQSRLSRSEKLATLGQVAAGIAHEIRNPLVGIGSTAALLLDEFSEKDP
                     RRDDIDIILKETKRLDRIVNQIVEYARPRNLAPIRFALQELVDEVVKLLETPLQAKCL
                     MVKRSLSPTLSLLHADRDQIKQVLLNILHNAIDASSAGGQAIEVTAYELPRNECPGLT
                     VKVSDAGVGISADALSHVFEPFFTTGKVHGTGLGLAICRNIIDSHAGEIHMTSEVGKG
                     TTVRIWLPLSQDLKGIS"
     gene            26000..26368
                     /locus_tag="VLD60_06960"
     CDS             26000..26368
                     /locus_tag="VLD60_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44732.1"
                     /translation="MRGRTRKWAVVLVLSLAFSALGCIRASGPLDLTSQNPYYDQEQI
                     ASYYTREANNFKLKAQELSQRASVYEGLFGSDSEWVKGTRLLEQFYEYSAQEQERLAA
                     EHLDIAGRERQSLMVRPAGP"
     gene            26403..27293
                     /locus_tag="VLD60_06965"
     CDS             26403..27293
                     /locus_tag="VLD60_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HSB44733.1"
                     /translation="MSRLIRHSLIVLLPLFLLLSTACAGTSVQPGQRGLMWHPFSEGL
                     STEPLKDGYYWRAPWNDIYVYDVRWQSFTENVDALSADDLQVLVKAAIILRPIPEEVY
                     FLAHELGSDFYPRVVKPEFLAAVRSVVSGYSMVTVPEKSAEIASKVQAVVVEKLKGRH
                     LEIQSVALADVDFPQVVLSAIEQKQAKEQEKEQKEFELTIAAKDAEIARTRAKGEGDA
                     IRIRADGEAEGLRIRAIGQAKAQETITKTLTPDYLRYKLYDSPNSKLVLLPDKLNVPL
                     LVNPGADQPHQLLPAESHAR"
     gene            27598..29220
                     /locus_tag="VLD60_06970"
     CDS             27598..29220
                     /locus_tag="VLD60_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44734.1"
                     /translation="MIRKSFKGLLSLVIVTAIALIVLTIATVNLVDRSVIEDAAAEDR
                     HRAFLLAAGAVSRIISQSGDIHNVNALEAAIHDVLTLRPGLRRLSVFDARQTNGALIW
                     SSDSERAPKSLTPHELTEVRAGRSASYFDTSTNDRAWVIAAPIMMNGQTIGALRGHFS
                     VAKFDRIAEEEQTQAGLIAIAMVALTCLAFFLLIQRQVHRPIRRLLAAMHRAEAGDLT
                     GGVSLIGPRDLREVSRQFNQMLDRVRAAMDDKEKLLEEIKDFNGTLTTRIGEAKQELQ
                     QTHAMLVEARIQAERNEKLAALGELSAVMAHELGNPLNSISGHLQLALKEGPQQEHHR
                     HLTIIRSEIDRMVTIIQHILESTRLHVQSASVDLNKIIRDIQSLIAPGLSEKRIVFKT
                     DLAEPLPPVAGDQRSLHGMIFNLVMNAVQAMPHGGELGVKTLRAIDHQEEGTLVLRGD
                     TALKKGAVRLMISDTGHGIPSDRLTKIFEPFFTTRQNAGGTGLGLAICHRVVSSVGGR
                     IAVRSAVDEGTSFTVDLPIWKEPRGGKPHHES"
     gene            29210..30613
                     /locus_tag="VLD60_06975"
     CDS             29210..30613
                     /locus_tag="VLD60_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB44735.1"
                     /translation="MNRDGCILIVDDDQRNREMLAEALQQANFNVELAASGEEAIDKG
                     GRREYDAVFSDIKMTSVSGMEVLDAFRTTAPETPVVLMTAFGSVETAIQAMKQGAFDY
                     ITKPVNLEELILVARRAVEHCRLVRDHRHLQHAFSERPRAASIIGQSRKMVEVFKIVG
                     KVARSRTAVLIQGESGTGKELIARAIHDNSPRAAQPFVAVNCSAIPDSLLESELFGHV
                     KGSFTGAHAFRRGLLEESSGGTFFLDEVGDLSPAGQAKLLRALQEGEIRRVGSNEAVP
                     VDLRVIAASRRDLHQLVKAGHFREDLLYRLNTVTITLPPLRERIEDIPLLAEFFLAQY
                     SAEKQATTASLSANARHALLRYPWPGNVRELAHVMERAVALASHSVLSLDDLPPEIRD
                     SGSGQRDLMKGLPGTLSALQREHVLKALDSTRGNKEKAARMLGISRRTLYRFLDRYGL
                     HRTPPSSDAETLDSTAS"
     gene            31136..31543
                     /locus_tag="VLD60_06980"
     CDS             31136..31543
                     /locus_tag="VLD60_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44736.1"
                     /translation="MEGYGKRVLVVDDDEHTRLLMGTLLEGAGYNVVAACDGLDALVE
                     IKKRHFDVVITDLYMPNLNGIELLGRINGIHPEIPVILASGCLSEFLGTRKASQFFAC
                     LRKPFENAFFLEIVRVAAHVSDKTRAEGVSAWQ"
     gene            31926..32762
                     /locus_tag="VLD60_06985"
     CDS             31926..32762
                     /locus_tag="VLD60_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB44737.1"
                     /translation="MAAVPDPAMGSHEQAEPEERRTVGIFEKLAPATLFLSVTYDSAH
                     PLASLTQMGVGAGFIVDDTGRALTNAHVVDGASTITATLYDGHKVKAELLGWDPISDV
                     AVLQLEKHDSPFLPVKLGDSSQLRVGQHVLVVGNPFGLGFTLTSGIISGLGPGTSVIE
                     STNKRLVQTTAPINPGNSGGPLVDSHGSVIGMATATLMGAQNIGFAIPINVAKDVLTK
                     LRDQGTIERPWLGITGQFVTDEVQRLFALPLPKGMLVVDVDDGGPAVDAGLRSGTLHV
                     TI"
     gene            32847..33041
                     /locus_tag="VLD60_06990"
     CDS             32847..33041
                     /locus_tag="VLD60_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44738.1"
                     /translation="MNALHVGQKVPIEIVRDGGRLQGSIMIGELPVGALKSKSGPGQQ
                     VAGAMFPGTMAGPHYAAVSF"
     gene            33068..34474
                     /gene="sthA"
                     /locus_tag="VLD60_06995"
     CDS             33068..34474
                     /gene="sthA"
                     /locus_tag="VLD60_06995"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HSB44739.1"
                     /translation="MAQNAQCDFDLLCIGSGPAGQRAAVQAAKFGKRVALVERRRLLG
                     GVCVETGTIPSKTFREAVLSFLCAANHREQHLGNHFDSRPTAHQLLSRVEEVTKREVE
                     VVADQLRRNDVVLIQGEASFKDPHTVLVVNEHDSRIVTAANILIAAGTVPAFPDGVPV
                     DGEVIMTSDDVVRLTRLPRKMVVVGGGIIGIEYASMLAALKINVTLVDKRTRLLEFVD
                     SEIVEELMHQMRNRNVTFRLGEAVEKLELTQEEPRRAVLILESGKRIVAESVLYSVGR
                     VGAADRLNLAAAGLTADKRGRLSVDGQFRTEVPHIFAAGDIIGYPSLASTSSSQGRHV
                     ACHAFGVEIDPLPEHLPVGIYAIPELSMAGPPEHVLTEQRIPYETGVARYREIARGQI
                     LGDDSGLVKLLFHREDRRLLGVHAIGTGATELIHIGQAVLDLNGGLDYFLRAVFNYPT
                     FAECYKVAALDAFNKLSM"
     gene            34511..35101
                     /locus_tag="VLD60_07000"
     CDS             34511..35101
                     /locus_tag="VLD60_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44740.1"
                     /translation="MRVLLSQVVKKQRGNAVFVIVAILGPCLLLAACASVDTILLTSE
                     KFPPKKSADEVAVLEQKPTRPHVDLAELRIGDSGLSFGSLQHKILDKAASLGADAVVF
                     AKPQTQTTHEVAYQPLYDPWGYNSPYYGSPWGYGGYGGPYGGWSPWGGGYSGSIAVPY
                     DETMNMLMGTAIRYIEATTAGGLKESAATQLGRQTQ"
     gene            35169..35648
                     /locus_tag="VLD60_07005"
     CDS             35169..35648
                     /locus_tag="VLD60_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44741.1"
                     /translation="MVIGCVALLCCSCAMLTACVSVRVEPLTHESYAPRADETQVQWL
                     ETAPGSPYIKLARIIATSQSADEDRMKDKVLARAATLGADAVVGGKFDILESIGSGPL
                     YESTLGPAADSFSPYASGWGWWNPFYYDPWSFVQGAADQTGRTEYLSGTAIRYANQK"
     gene            35816..36301
                     /locus_tag="VLD60_07010"
     CDS             35816..36301
                     /locus_tag="VLD60_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44742.1"
                     /translation="MKRHRLWTLVGFTLAMGIASCAGPNQTNQESTPPDDPVIGLLSK
                     GITQLNANINALSKRMNDVRQASAGTDPVLQELQALDLSGWQLHQQQWVLQRNHLVLA
                     RDLLQRASKSQGTNGPLLDDWRKHRQEYMQAIEDLRQQRQTLENKHLEVEARLVERGL
                     Q"
     gene            36413..36616
                     /locus_tag="VLD60_07015"
     CDS             36413..36616
                     /locus_tag="VLD60_07015"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB44743.1"
                     /translation="MKKRGNGKNLVKASPDMQSESLNTWNNRTWDRIATKAYELYEQR
                     GRIDGYALDDWLKAETMIHGTTA"
     gene            36833..37120
                     /locus_tag="VLD60_07020"
     CDS             36833..37120
                     /locus_tag="VLD60_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44744.1"
                     /translation="MKRLLALFGFTLISTIGIATLSYAAGSKLVEGDVLKIEGEYYTV
                     HDTAGHDERLHVDKTTHLEGTFKVGDKVEVYATDKGHARAMYHLTHIQPAP"
     gene            complement(37509..38048)
                     /locus_tag="VLD60_07025"
     CDS             complement(37509..38048)
                     /locus_tag="VLD60_07025"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44745.1"
                     /translation="MIILAGAIGLVLLMAPLVGVAEVSKELVVKYILATVQAFRTVYV
                     EQVTERVSKEGILPKEDWMKDDHAVMLPFQFVKMAGQEMKTLEKDVEIGLVSLTPLYS
                     SNFPKTETEVNALKELMADPTRKIMTFADGKDFKGLAADFAIERSCVDCHNHHPNSVK
                     KDFKKGDMMGAIVVRLKKS"
     gene            38462..39028
                     /locus_tag="VLD60_07030"
     CDS             38462..39028
                     /locus_tag="VLD60_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44746.1"
                     /translation="MLAWLNKWGDRELFWVMLALIGLVVFGLNALAEAAPVGPSDWEL
                     ATVVAGRDTAPEVERKLGLSPCLVPNSTGDTISYLYNVPGTKGPNYLRLDVNGHVDAI
                     TVSQDPPIVGVCYAPTRASLPVRTAKGIELGATIDEVMKLYGEPTERFAVGPMARFRY
                     VATYDRSYEWDLVFRNGRLVEWTVSTED"
     gene            complement(39032..39550)
                     /locus_tag="VLD60_07035"
     CDS             complement(39032..39550)
                     /locus_tag="VLD60_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44747.1"
                     /translation="MVGASVMRSPLGVGTLCAIMGLMLQACAGGQHNGLAAEALLTPE
                     GFATWEREEAALKGNMLAAKESYKQAGSLDALKSYEGAVREYLDHGFALYRAYQDVHR
                     PFPPDLVSSLEQRTNLLMDVADEYVKHGSTAVGESIASEVVRNYSDLPVMAPAQRRAE
                     ALLLRYRYRQDY"
     gene            39765..41354
                     /locus_tag="VLD60_07040"
     CDS             39765..41354
                     /locus_tag="VLD60_07040"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSB44748.1"
                     /translation="MAERTREARKRLKHSEFATSTQPESIERTVPLPPPTKDGRDDIS
                     DVDLQEVDELRKAFQEMQSQATKEGAPASEKSGPQEGPSPPPSQPGPKKELDDEIKSL
                     TDRVNALERVLVGTEERPGLAGLIGGWTRHNGFFLMSSNGDFFLRLPVLFQSDYRSFP
                     GGQNGTDPGVHPNTFLLQRVRPMAHVRVWRYFHMLITPDFGNGVIDQNPSQNQSVVQI
                     PDGFGEWDYFSAMRIRVGKFKSPIGLEMLQGIQNLSFMERSLTRNLLPNRDIGAMVWG
                     TFWRGALEYQIGVFNGAPNANFYQDTKAASSDKTMEARVFSRPFVDHSVEWLRGLGVG
                     VGMSTGSVSNTSGQEIMQTETLSYTFFNYGSQVQGDGNRFRLAPQAAWYYKRVGLLGE
                     YVSSTQHLKNGNGPDRRTTNDAWMAQMSFLLTDDTSTYGHIEPRQPFNWSKKQWGAWE
                     VAARYAQINLDQDNYAFNLADPTVNVLRAKSTSIGLNWYLNSNVRITGNFVHTDFTGA
                     TPAYRAADHENGLMFRAQLTF"
     gene            41358..42827
                     /locus_tag="VLD60_07045"
     CDS             41358..42827
                     /locus_tag="VLD60_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSB44749.1"
                     /translation="MATIWLFLFVLACANVASAEDPLPSQQADTAPPPKTSAEPEVES
                     SPVQGPPDPTKARQRLRELDRRLSVLESVAVGRGHGRGVMERGLGWSPEQRERGFALY
                     STDGSYRLHLNGGVQTDYHTYPMGHNAIDPGTQENGFELRRLRPIMDFRVGQFVRGQI
                     MPDFSSSFQLFNAFIDVEPSDWARIRIGLFKPVMNIENQQGEFDLVFAERSMVQNFAL
                     RRDFGVEVTGRVLQQSVRYDIGVFNSNNGAIGNNSRPNVSADSNKLGYARLMYTPFLR
                     KGPQIFRKLDVGVAGFYGNCSQSACQQPMQTMGFDRTIFSYNGTVTGNGAETKILPQM
                     SWFWNRIGVMSTFIHSWEPKIDHASGRSDTLQHQAWMIQGEFALTDDEPAFNRVAPKH
                     PFDATWNSFSRGDWGAWTIAARYSEQDLDPNTFGLNYGNAAIYARTAKGYSVALNWYA
                     SREFRLQGIWEHTDLKGANSTYIASGTSDFVIFRVTLIY"
     gene            complement(42979..44469)
                     /locus_tag="VLD60_07050"
     CDS             complement(42979..44469)
                     /locus_tag="VLD60_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB44750.1"
                     /translation="MTIEPAKLRSPMRRESNMGGFEPARLSNARRVLPGMWCMLIAVL
                     GSGELCWPAERSAEEPVRSLVPVSSPTAPQTRGGFLGYEAAIRIALDRHPLLRRSKET
                     ASAAEAVTGQAEARFYPELNAYAIQTGGTVRPLSAFNVAGAQNKPTSYIESAGFRADQ
                     LIYDFGQTAHKVLAERSGQEAAEKDIQTHKATVILKVQQAYINCLRQRRLLEIAEETV
                     RERGVLRDQIATLYKRELKSKLDFDLISVELRNAEVQLLQAKNELRAAFAGLNNAMGV
                     RGSEDYTLEDLPAAAASAYTLESLITQALEDRPELLGTTDRIRQADERLSSAQALNLP
                     TIAAQGMYGVIHFSDAPLNQYAGSHPGQTNLWWGAGAMVSVPIFTGFLIENRVAEARQ
                     QKYKVEQKKVNLSNNIALEVTDAYLSLQAARQQMVVEEKEVESARSALTLAKERYRLG
                     LASIVDVTTATTALLVAEVRLSEARYGVQAASVAVAYATGQASQNY"
     gene            complement(44405..45757)
                     /locus_tag="VLD60_07055"
     CDS             complement(44405..45757)
                     /locus_tag="VLD60_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB44751.1"
                     /translation="MTGLLLCIISGAATLFAAEDTSLPLPSAAGSRLSWEQAIRTAIE
                     KHPLIKVAEHEMLESQALIKQIESANYPQITGLYSNSAGNTRVLANLNVSGSLPKPTN
                     YLTTPGIRADFLITDFGQTAHRILSQKSFAASAEKALLTSKALAILDVEQAYLNCLKQ
                     QRLVEIAREVMNERDLIRQQTEAFYRRQLRSKLDLDFASVEFNRAEVVLIKAQNNLAS
                     AFTALNTAMGLQGPGTYVLEQAGLSVIPSPTPESLVQEALNQRPELLGSKDRVQAAEE
                     ALKAAKALRFGSVTSIGTLAYTWWGREERPSGKDVSNPGAKLGWYGVGVASSFPLYTG
                     GRIEGQIEEADARKGETQATTRSIANDIVLQVAQAYFSRLTAEQQIKVAEERVAHARE
                     ALTLARERYKASLGSILDVTTATTDLLSAEVGLAEAQYDYRASEAALAYATGVEYGRF
                     "
     gene            complement(45762..47183)
                     /locus_tag="VLD60_07060"
     CDS             complement(45762..47183)
                     /locus_tag="VLD60_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB44752.1"
                     /translation="MRQTFIGILVLALLANSLQVALAETGEFTSPVEGAVSRPVVKPA
                     GSLLKLDEAIRTGLATHPLIERSRYSSLIAKALTRQTQGERYPWLEASVAGTSGSLRI
                     VTTDGKTIHDRGGHGFDPGGALAKHNQNMLTGGFMLNQLISDFGYTAHRILANEANEA
                     ASEKEISTNKAFVILNVQKAYLNCLLQKNLVLIAAETVKQRQAVRDQVQGLYKHQLRS
                     KVDLDLMLVEVSNAELSLIKAQNDLKQSFAALNNAMGIEGPERYELEQIPIQVTPSPA
                     VETLVTTGLSDRPELLGNRDRLVASEELLKAVKALNFGSLSAVGSLGITKYGDVHDAG
                     IPSDGVAPFWGFGATMKFPIFTGFRIQNQIKEASHRKGETEEELQNLGNEIVLQIVRA
                     YLTRTTNAEQIGLEQERVTFAKEALMLAQERYRLGLNPIVEVVRATAALFESESRLAE
                     ARYIYKTSESVVSYATGQEYKRF"
     gene            complement(47233..48438)
                     /locus_tag="VLD60_07065"
     CDS             complement(47233..48438)
                     /locus_tag="VLD60_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB44753.1"
                     /translation="MAAPSQLSMGEDLMMQPRTMKRLYIVMGSVLAGALIVIMTLVGP
                     NGREESVSSPPRDSKGTGEAEGGPAVQVLKPTRRDIARTLTLPANISPWQQATLYGKV
                     SGYLKWIGFDKGDIVEKGQLLAVIDAPEIEDQYKQADADYAIKKVTYERYLSVWKDNP
                     DIIAKQDVDVAKAAAESAKHQRDSRRNLMDYTKVYAPFSGVITARFADPGALIQSATG
                     SATQAAPLFTIMDLSKLRVYVSVPQETALLAKPGIPAVLTAREVPGREWKVSVTRTTE
                     SLDPATRTLLVEIDIQNEKHELQPGMFVTAKLTLEQHPNSLALPPAAIVSGKTRDEKW
                     VFVVEQGKAHRNPIRTGIDDGVSIEVVEGLTGEEQVVVVGKSGLSEGQAVTVSNYDLP
                     AGQNAKQKM"
     gene            complement(48512..>51581)
                     /locus_tag="VLD60_07070"
     CDS             complement(48512..>51581)
                     /locus_tag="VLD60_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB44754.1"
                     /translation="VEGAITLRLEQLFLQASYVEHIESRSLPGVSLIKIYFHPSYDVN
                     AGLAEITSLTYSGLRYLPQGIFPPVIIKFGAASLPIAVQTTSSETLGEKEVRDLAYFN
                     VRPQLGNVPGIAFPTTFGGTVRQITVFLDPDRMQARGISTQEIVNSVNAQSVLLPAGN
                     VKIGVFDYNVYTNSMIKIVEQMNDIPLKVVNGVPITLREVGKAVDSTMIQTNVVRIDS
                     HRAVYLPIMKQAGANTIQVIDGIKAVLPKLLALPKELSVKLLSDQSMYIRQAIQTLEH
                     EGLLGGGLACLMVLIFLASLRSTLIIALALPLSIMAAFVLLYFTGQSVNVMTLGGLAL
                     VIGTLLDNNIVVLENVHRHLAMGKSPEQAAGDGTSEVALPMLVITISILIVYLPIMFF
                     TGIIRFLFVPLALAVAYTMVVSYFSSMTVAPVAMAALLKPAGHAGGGDHAASSRLFDR
                     VFGHLVNRYVQTLRWCLRHKALVILGVMVIFVVSLLAGTQLATEFFPKVDSGSFILNV
                     SVPEGTRLEKTEAIVARVEDLVRQEVPAGDLEQIVSNIGLPQGWMVLYTPVNGPHQAF
                     IIVSLKKEHTTRTDAIIEKLRGVLLSRLPGLKFSFQTGGIVSDVVNFGVPAPIDIKVT
                     GRDLSTLAEAANQIRNVVARVPQTTDVQVRQGMDYPEIHLDIDRAKAAYVGLKEKDIV
                     SDLITGLSSNVTLNPGYWIDPRSNNAYFVVTQFPEQLLVEFQDFLNLPLVGSPVSLRP
                     SVAVGGTLRRGGSLTRSQTPFPERPELPILGGNNRHSIMLRDIAQVTRTSGPETVDHY
                     NLQRTIDVLANVKGYDLGRVAAQVEQAVAGVSLPKDVTVMFKGEVDSMRSAIKGFAGV
                     LPLAIVLVYLVMVGLFRSYLDPLIIIFAVPLGFIGVIWMLLLSHTSVNVESLIGTLMM
                     IGIVVANSVLLVDFANNLMREGMPIEQAVVEAGRLRIRPILMTALATVFGLAPMALGI
                     GEGSEMNMPLARAVIGGLLVSTVMTLLVIPVLHALIRGRSTGHPVPQVTG"
BASE COUNT        11480 a        14991 c        13979 g        11131 t
ORIGIN      
        1 taccgtgctc gctgggcagg tttcggctgg catgctctcg tcattgacgg gcacgacctt
       61 tcggccatac ttgccgcgtt cgatgaggcg tcgcgcacca agaacacacc aaccgttctc
      121 cttgccaaga cattcaaggg cagaggcgtc tcctttatgg aagaccatcc cagttggcat
      181 ggaaaaccgg tccctcaagg cgcagatacc cagaaagcga tcgatgaatt gaccaaacag
      241 ttgaaaccga acggcgcgac gttgacgatt caaaagccat cggctgcttc tgtcgccgcc
      301 tcgttggtca gcccgcttcc cgcctcgccg tacaagccag gggaatccgc cgccacacgc
      361 gaagccttcg gagtggcact tgaggcgcta ggagccgtca atccatccgt ggtgggtctt
      421 gatgcggacg tcaaaaattc cacctacact gacaaattcg gcaagaagtt cccgggccga
      481 ttttttgaga acttcattgc ggaacaaaat atgctcggcg ccgcagccgg ccttgccgcc
      541 tgcggcaaga tcccctttgc agccacattc gcagccttct tcacgcgcgc ctatgatttc
      601 attcgcatgg ccgccattag ccaatccaac attaaactgg tggggacgca tgtcggcgtg
      661 agtatcgggg aagatgggcc ctcacagatg gggctggaag acatcgccat gatggccaca
      721 cagcccggca tcaccgtcct gtacccgtcc gacgcaacgt gtatgtatcg tcttgtcgag
      781 gccgctgcga accataaggg gatggtctac ctccgggcgg gacgccccaa atctcccgtt
      841 ctctatggac ctgcggagcg ctttcaaatc ggcggctcga aaatccttcg ccagagcgcg
      901 gcggatgcgc tgacgattgt cgcagccggt gtgacacttt tcgaagcgct caaggctcac
      961 gatcagctga aagccgccgg tatctccacc aggatcgtcg acctctatag tgttgttccc
     1021 atcgaccgag tcactctctt ggatagcgcg agggccacgc atggccgtgt tctgacggtc
     1081 gaagatcact atgcgcacgg cggcctcggc gatgccgtgc tcagcgccgt gggcgcggaa
     1141 ggtatcaaag tccataagct cgcggttcgc acgattccgc atagcgggaa accggacgaa
     1201 ctcgtcgacc actttggcat cggagcacga tcgattgtcg aagcagcgca acaaatcatc
     1261 aagtaatcga gctggagccg ttccccaggg ccagctcgct cagattccac tcctcaacat
     1321 cctgagcgga caggaccggc aacgcctcct ccacgagttg actgaagccc gttatggcaa
     1381 gggcgagttc attttccgcg aaggcgatcc gacggaatac tttcacatcg tgaaggaagg
     1441 cacggtcaaa tgcgtcaagt ccaacccgga cgggaaagaa tgcaccctca agatgttgat
     1501 gcccggagac ctcttctgct gcgacgccgc ggcttttgac ggagcccgcc atcccggcag
     1561 cgctcaaccc atgggggatg tcagcattct tcgcatgagc aagaaagcct attttgacat
     1621 gctaagacga agcccggacg ccgcgcttga agtcatcaag tacctgggca atcgattgaa
     1681 cgaggcgcag gagaaaacca aggttttggc gttggaacgt gccgatcaac gcctcgccgc
     1741 actgctcgtc gatctgggca cgagaagcgg tatcaaagag gatcagggaa tcagacttcc
     1801 catgcgtctg accagggaag acatggctaa catggtgggc accacaacag aaactgtgat
     1861 ccggatgatg agccgcttca aacaagatgc cttagtcacc ggcactgcca accgattgat
     1921 catccgcgac atcgagcggc taaaaaagct cgcgtcgacg taaccccgct actcccaagc
     1981 cccgcaatta tattttcaaa acatgacttg cgtcatgttt tatattgtgc actttcgcta
     2041 tcctccctgc caacagacgg actcagtcca ccacaccaat gacgatatgt ccgttacggc
     2101 cgaaccctgg gcgcatttat ctgttcatga tcagaaggag gagagatttc atggaaacga
     2161 tccgttcaca ccggtccctc gctcttgcag caggactcgg gactttcatg ttagctggcc
     2221 tgatggcacc agccacaccg gcctctgcca aaacccacga ggtctccatg accgcagtgg
     2281 aaaccgacgt cgtcattgac ggcagtggtg aaaaatacgc agcctggacg tttaacggta
     2341 ccatgccagg cccggttgtc cgcgtgatgg aaggcgatac cgtaaagttc acgttgacga
     2401 atccggcgac caacaagtac ccgcacgcaa tggacttcca cgccgctgag atcgactttc
     2461 ttaaaaacta tcgggcgatc aacgcgggtg aaaccatcac ctacaccttt gtcgccaaga
     2521 aaccaggcat tttcttctat cactgtggcg cgcccccgat gattcagcac gtagctcgtg
     2581 ggatgttcgg cgccatcatc gttgacccga aagatgccaa ggccttgccg aaagcagatc
     2641 gcgagtatgt cctcgtccaa tcggagtact tcaagaatcc cgacgatgtg caagggatgt
     2701 tcgacaggaa atttgacggc atgatgttca acggcggcat cttcaaatat catcccttcg
     2761 tgacgggggg aggaaaactg gatgcgaagc ctggtgagcg cgtgcgcatt tacttcgtca
     2821 acgcgggacc gaacgagttc tcctcgttcc acccgatcgg cgaaatctgg gacaacgtct
     2881 atgagagcgg caatccggcc aacaagctga cgggtgtgca gacctatgta gtgggacccg
     2941 gcagcgcggc aacgttcgac gtcgtcgtcg aatcagctgg agcctatccg ctcgtcaccc
     3001 attcgctgac cggagcgctc cgtggagcaa tcgcggttct gctggcgtca cctgacgcca
     3061 aaccggcgcc gttgatgcct atggtcccct ggaagccaac gacacctcaa acagagatta
     3121 ccccagttcc agcgcaatag cgacacgccg gacggcttgg ctgtattttg acagccaggc
     3181 cttccctgtc ttccttacta agccggtcat cccgatacag atttctagca gacagatcat
     3241 gaaatggcag agctgggcac aagatacgaa ctgattggta tttgcgaggt ttcgcatatg
     3301 ggattgggaa ctggctcatc aactccaccg gctctcgact cctctctgcg agggttgtcg
     3361 tcgacaatga aagcactcgc atctctgttt ttgataatca ttggcacgat cttcgtaact
     3421 tccttcacat tcgcacaatc tcccctcgaa cgcgtcacat tgatgctcaa tggctccgcg
     3481 tgcgaatcgt ttagcccgac ccttgaagta gcgttgaagg aattgccagg agtccggaca
     3541 gtcgatggca acagtgtacc aggccacctc ttgatagacg tcgagcgaga tctcctgaca
     3601 gccgatgaac tggcccgcag agtgatcaaa ttccttgccg cacagagtct ctgccaggtc
     3661 agtgtgatgc gatcttgcat cactgccggc gtccactagc gcactgggtt ctggcaacca
     3721 cgccggtgag aagaggcacg gggattttca acgatctatc taccaattgc ccgctgtgat
     3781 ctgtatgacg cgggcatgcg gattccccca agtgcaggat tgtaccgtgc ccccttcttc
     3841 ctgatcgatc catcaaacaa tccccgctgg ccgttccaca ttgcatccat attcttcatg
     3901 ttcttcacca ctctgccaat gctcagcgct gtttcttacg gatcagcagg tttctctctg
     3961 tttgcttcat cctgctgtat tactagaacc cctgtcactt tgttccagtt ggcggcacca
     4021 agagagcgta ttcgacggag gaatacaaac caggcgacgg aggagatggt ttagacggag
     4081 aacactattt tcgagaacga caccctgaca gccataatgg tgtgggagga cacttgagca
     4141 ggagcaggaa caagaacaat ccaatgaaag ccaatccagg gatcaagcat tccatcttct
     4201 tcgaacatat cagatagctt atgccattat ggaggatata tatcggccaa caaatgcatg
     4261 atccaggctg aaccagcccc gatcagtaga ccattacatc tagtctcatc acccacttca
     4321 tgattcgcag gaagcagcat tccaaaccgc atgagcgaat ccgcacaacc cgctcatcgt
     4381 gaatccacac aagaagttgc ggttcctgcc agtcgccagc ggcggtcttg ctgcactcgc
     4441 ggttatttgc tgcaaaacag agataatcgt cttgtccgag cctattctgg ttcgcacaac
     4501 ttacttggcc ttaaagttgc ttaacgctta ccggcagcat acacgacagc ccgataacaa
     4561 aaatggagcc gtgaaaatgg actatgtgaa acctcaggag atcgtgtctg acatggtgtc
     4621 aaccggtgcc ttgaagttgg acctgccggt ccggcatctg ctgatccggg gagccttggc
     4681 cggcgcatac ctcggggtgg ccaccagcat ggcagtgact gcggcggttg aaaccggcag
     4741 ctggctcgtc ggctcgcttc tctttccctt cgggctctgc ctggcggtcc tgctccggac
     4801 ggaaatcatc acaggaagtt tcgcgctgct cccttgtgca accgccgatg ggaaagcaaa
     4861 cgcctgcgtg agacgcgtcc tcatcaactg gggctgggtc ttcctgggaa acctgatcgg
     4921 aagcacgctg tatgccggac ttttagccat tgttctgacg acagccggag acgccggtgt
     4981 ttcagcggcc ggcgcaaaat tgatcgcgat cgccgaagca aaaaccaatt actacgcgtc
     5041 ccatggctcg gccggcatgc tcaccgtctt tacgaaaggc atcctgtgca attggatggt
     5101 gagtctggcg gtggtcttcg cattcgcaac aacttcactg tccggaaaga tcctcgccat
     5161 ttgggggccc accgtcctct tcttttcaca aggatttgaa catgccgtgg tcaacatgtt
     5221 cgtcattcct gtcggcatgc tcttgggagc ccatgtcacg gtcaccgatt ggtggttctg
     5281 gaatcagatc cccgtcacgc tcggcaatct ggtcggcggg atgatcttca caggactggc
     5341 catttacttt acacatcgcc ccactccggc tgtgtcagaa gcacgcgtga gcgccgttcc
     5401 agacaatgcc cacgagaaac gtccgaggta ttcgccgtcg ttctgaggct tcatgaatga
     5461 gtccctcacc gatgtctgag tcacattcca cacgcctcgc cggctggcaa tttttcctgt
     5521 tcaaccttgt cctcggcatc gcgcatatgc tggtgctgtt caatgccggc tcctatgtgg
     5581 cgctactgcc gcatgctgcc ggcaacctgg gaggcgtgct gccgagtttc ggcacctggg
     5641 cgcaaacgga cttcatgatt gcgctggcct tggccttccc gatcgcccgt tggctttcct
     5701 cccgattcgg ggaagtccgg gtatttatcg gcgcgttcgt tctctatgcc ggcgcctcct
     5761 tgctctgcct tataggcgac acgatcacca cctttttacc ggcacgcatt ctcctcggcc
     5821 tcgccggcgg tctgaccctc ccgctcggac aaacgctctt gctcaaagaa tatccggatc
     5881 gactgagatc tttggggctg gcagtttggg gtctgtttac gttgatgccg tttaccctcg
     5941 gtcttgccac cggcggctgg attgcagacg agttgggctg gcgtgcgctc ttcgccctga
     6001 acatcccttt tgccctggcg atcgccggcg tcactgccgc attactttat agacgcacgt
     6061 tcgtaccgca tcgcgagcga ttcgatctgg tgggatttgt tctgctcacc gtgataatga
     6121 tcggcattca aacgattctc aatgaaggca acgattttga ctggtttgat tcggcgctcc
     6181 ttagcggaat gctcgtggtg gtgctcgtcg ctatcccggt ctggattatt tgggaactcg
     6241 gcgaacgcca accggccgtc gatgtgcgcc tcttcacgca tcgcaatttt gtgatcggcc
     6301 tgctctgcct cgccttgggg ttcttatcca ttcagggatt gcttgcactc ttcgtcgttc
     6361 agttgcagac gttgatggga tactcctcgg aacttgcagg catggttttc cttcctatga
     6421 tcatcctggg cgtacccctg atcgcggtca tgcatgaagt tgccaaacgt ctggatgtcc
     6481 gtctgttggc ctgcctcaat ggccttggat ttgccgccac gttttattgg atcggtttat
     6541 tcgacgatcc gcagtctttc gatcaaattt tctggccgat ggttctggaa ggcgtttttc
     6601 tcggttcctt ttttacaccc ctgaccgtgc tcaccttgca cggcttatcc ggcgaccaga
     6661 tgcttcgggc ggcggaagcg gccaacgtct ttcgtattgc agccggagcg gtgggcatta
     6721 cctggcaggg cgttgtcgta ttccggcgtc ttccctttca tcaattgcaa ctcagcgagc
     6781 atttcggcgg acgtatgtct gcgtcgtatg acccactgca gcagattacc ttgaaactgc
     6841 aagacaccgg actcgacccc gtcatgattc agcgcaagct gcaattgctc atgaagcagg
     6901 aggcgggtat tctggcgctg aacgatgcct tcctcctgtc cagttatctc tgcataggac
     6961 tggccgtgct cgtctggttt gcccaagcca gtcgtgtccc tgtactgaaa cccgtcgagg
     7021 ccgtgcgcga gcttcaggcc gaagaactga tggagcaacc atgatccgaa cacggagtag
     7081 gttgaacggc cttttcctga acggtctggt cgccggagca ctggtgatgg catcaggctg
     7141 tgcctggatt cccaagggcg atcctcccgc cgaatatcta gagccgccag aaatgaaaga
     7201 aaccctggcc gaagtcacga gccgactcca acaatggccg gatgaccaat ggtggaaaca
     7261 attcggcaat caagaactga atgagctgat cgatacggcg ctcaaggata acccgggatt
     7321 caaagtcgca tccgcccggc tgcgtgaagc agaagggctg gtgaaggtcg aaggggcacg
     7381 actgctgccc ttcctggaag ccgacgcgtc gctcacctat gaacgcattt cacagcacgg
     7441 cgtgtttgcc gcgctgaacc ccaaagtggc gggggacaag atcttattgg gcgtcatcaa
     7501 tccgttgagt ttccgctacg agttcgattt ctggggcaag aaccgggcga cgctggaagc
     7561 ggcgctgggg ttggcggccg ccgaggaggc agaaagggcc gaggtgcgcc tgcggctcac
     7621 caccggcatc gcgcgggcgt atttccgtgg acaggctctc cagcagcaac ttaccgtcgt
     7681 gaaaaccatc gtcgccatcc gtgagaactt gcgcacgctt gcagaaacgc gatatcggct
     7741 cggtctggat aacgaccagc ccgtgaaagt agccgtggcg gactatgaag cagccttcaa
     7801 acggcaagcc ggtctccaag atcagctcga cgtgcaacgt catctgcttg cgcggttggc
     7861 cgggaaagga cctgatcagg cagcgcacct gtttttaaaa ccggtggtca ttccgggtca
     7921 aattgcggcg cccgaccatc tatcgatggg gctgctggtc caccggcctg atttagctgc
     7981 ggccctgtac agggccgaat ccgctgcgcg gatggtcaag gtcgccaaga cacaatttta
     8041 tcccacgatc gatctgacag cgtttgtcgg gttcaatgca ctgaccctgg gaaagcatgc
     8101 cgacaagcta tccaatctat tgttcagcgg ccagagcttc gcatacggat tcgcgccggg
     8161 gctgcgcatg ccctggtttg aaggcggccg cctgcgggga gaattgtcgg cacatcgagc
     8221 ggaatacgac gcggccgtcg aactgtataa cgacacgttg ctcgatgcga tgagagaagt
     8281 ggcggacagc ctcagtgcct ggcaatcgac ccgcaagatg atcgaatcgc accggcgcct
     8341 gctggcctcc ctcggcgagg actggcgcct ggccaaggtg cggctcgtga ccggactgga
     8401 tgacgaccgt gaagtcttgc ggcatcgaca gccggtcctg gaacaggaat atgctttgcg
     8461 ggcgttagag agcgaccagc tcgttgcggc gatcgatctc attgaagcct tgggcggggg
     8521 ttaccacaac cctgatgttg agaaaagacc agcacaaaaa ccgagctaaa ccttgagcac
     8581 gacacattca atccagccac cggctccgga cctctatgca gactccctcc gctcacagcg
     8641 caatcgacgg ctagtgatcg tcatccttgc gctgggcctt gccggcctgg cctatggagc
     8701 ttattggtgg acgacggcgc gcttttgggt cgagacggat aacgcttatg tgacggggaa
     8761 cctcgtgccc gtcgccgccc aggcttccgg aatcatcacg caggtgctgg ccgaagaaac
     8821 ccagttcgtc aaccggggcg acatcctgct gcagctggat caacatgaag catacgcggc
     8881 gttaggccgg tcccgcgggc gcctcgggga aactgtaaga cgtatcaacg cgctgttcgt
     8941 aaccaagcgg caagtcgaag agaaactggc cgcccggcgg gcaagactgg atatcgtccg
     9001 acacgatgtg gaccggtacc gtctggcttc acccagcggc gccgtctcca aacaaatcct
     9061 gcagaacgcg acggaccagt tgcaggcgct tgaagcagac gtgcgggaaa cccaagccga
     9121 gtacgacgcg ttggacgctc aaatcggcgg cacaagcgtg atggaacatc cggcggtcga
     9181 cttggcaaag cacgaattca tcctggcgca tctggaatat gcccgtcagc aaatccgagc
     9241 acccgtatcc ggatatgtcg ctaaacgaaa agcccaggtg ggtgatcggg ttaaacccgg
     9301 aaccaatctc atgacgatcg ttccgctcga tcatctttgg gtagaggcca acctacggga
     9361 aacgcaatta gccgagatcc ggccgggtca aacggccgag gtacaggtag atctgtacgg
     9421 cagcaaacat acgtttcacg gtacggtgga gggcctggtg cccggaaccg gaagtccgtt
     9481 cgccttgctg cctccggaca attcgaccgg caattttatc cacatcaccg agcgggtacc
     9541 ggttcgtatc gcgttggcct cggatgagat cagggagttc cctgtccggc cgggcttgtc
     9601 cactgtaacc aggattcatg tcagtgagcg tgggaactcg gtgtggtcct cactggcgaa
     9661 gccggatacg gaggagtatc aaaccgatgt gtacggggat gagttgacca gcgcagaatc
     9721 gatggcgcaa ggtgtcattg caggaaacgt cgtcgtcaat gctgaattca gcagggaacg
     9781 atcggcagct accctttatc cggtcgcatt gattgatcag gatccggaga tttcatcata
     9841 cagtcccgag cacaaagacg accgttccca cagaacctcc cgacagagaa cgcaagagat
     9901 gacgcccgaa tcatacgttc cccccagaag tccagacctt ggctctgcct actttcctct
     9961 gtcaccatcg atcggacctg gttcttcacg actgggaccg gccgcagggc ctgatgcgtc
    10021 ccgccggaaa tttttgggag agacggaaac ccgtacgaat ggtatcggga ctcgaagata
    10081 gaggacgttt gtattaggga ttcggagcga cgaacaccag cactttgagc cgctggctcg
    10141 tgtgattctt cacaccatgc tcttccccgg ccggtgccat ggtaccctga ccaggtccga
    10201 gaacctgttt ttctgatcca acctgaaacg ttccttgtcc ctcaagcaca agatacacct
    10261 tatcctgctc tccgtggatg tgacccttct gctcttggcc aggctcgaaa caatacacat
    10321 cacagaagaa gcgcaccgtt tcaaatatat tgtttttctt catcttctca ctgctaaatg
    10381 attgataatc attcaattta ataacctgca tgctaatcct cccctcctca ctttgttgac
    10441 atgatttcgc ctggttttgt caccgggtcg gccacaggaa tcgaccaagc cgaccagctg
    10501 tgcctttgac cccctgatcg actttcggct acgctgtaac gagtggggca tcatgaatcg
    10561 atcagagcga cggcgtcaag aagcacagaa agggactagt catttttccg gccaggtaac
    10621 caccctgctg agtgagggaa agacctatca ccaagcaggc cgcttagtgg aagctgaaac
    10681 ggcctaccgg caagccctcg ctctcgcacc gcatcacccc gaagcacttc atctgcttgg
    10741 cctggccttg tatcgaggca atcgactcga ggaggctatc aattgcatca aagcggccat
    10801 tgagcaacaa ccctcatctc ctctctactg gttcaacctc ggcgtcatct tacaaaaagc
    10861 ctctcgccag gaggaggctc tcacggcgta tcggaaagcc gtggcgttga atccccgcta
    10921 tctcgaagct tacatcaatt tgggaaacgc actccgggat cgcggagacc tcacggaatc
    10981 cattgaggtc tatgagaaag cgttgactct taacccctcc aacgccgaaa cccataacaa
    11041 cctgggagtg gccctgaaag agcaattccg gaccgaccag gccattgcat cttatcgccg
    11101 cgcactcgaa ctgaaaccca ctcacgtcga agcgctgaat aatctcggtc tcgccctgat
    11161 ggaaacggga acattgcagg aagccatcac ctccttccaa cgtgctctcg cgatcatgcc
    11221 aggctaccag aaggcactct ataatcttgg catcgcgtgg tcatgggccg gagacgacgg
    11281 aaaatcagtc gactgtcttc aacaggtggc gaaagccaaa tatgatcacg gcaagattgt
    11341 caccgaatcg ctcatttatc gctcacgact caaacatgac atcgaacaaa ctcaatatct
    11401 cctcgatcaa cacaaactgg ggaacgagta ccgcccctac ctccagtcgt tgcgcgaact
    11461 ccaggagcac ttgaacagaa ccccttcgac acgcaaccgt acgaccattg aaccgaaccg
    11521 gttggctcca atcgctccct cattcaacag ggtgctctat tgtgttccac cgccgcggat
    11581 tgaacatggc gcactcaacc cgtcccttga tgcagcctcc gttgaaaccc gctacctaga
    11641 gaagcacccg gaggtgacgt ttatcgacgg cctgttgaac caggaagcct tgaacgggct
    11701 ccgccgattc tgctgggaag cgacgatctg gaagaaagac tatgagaacg gctatagcgg
    11761 ggcattcctc ggagacggct ttgcttcgcc gcttctcttg caaattgcag aagaactccg
    11821 gttgaagttc cctcgcattt tcacttacca ccggctcact caggcatggg ccttcaaaca
    11881 tgacagcgcg cgtcgtgggc tcaatatcca cgccgatgcc gcggccgtga acgtcaattt
    11941 ttggattacg ccagacgacg ccaacctgaa ccctgcaacc ggtgggctgg tcgtctatga
    12001 caaggaagcg ccaaaagatt ggaatttcaa agcctataac agcgaccaga ataagccgaa
    12061 aatcctcgca tggctggaac aggtaggagc caaggccatc aagatcccct atcgggccaa
    12121 tcgagctgtc gtcttcaact cagatctctt tcacgaaact gacgagattg ccttcaagga
    12181 cgactacctc agccgccgca tcaacatcac cctcctctac ggataccgca taagcgcgta
    12241 acaatggtcg atcgccaata gacgtggaat tggccgccct caggccgcct ccgagccatc
    12301 aggatcaacc tgcgtctggt agggatgaag ttcgagctgc gtcacggtat gttggaggcg
    12361 cgcccattct tccgggacga gggtgacaaa ttccaaacca aactgttgtc cgcgactcca
    12421 gcgaacgacc ccttcctgaa tcttgagcgg cggctcctct tctgaagttt ggatgcggac
    12481 ctccaaggaa gtccccggcc tcacttcagt ggaactgtcc acgcggcatc cacgaaggga
    12541 caaatcgacg atgcgcccat cgccgccgac gatatttaat gaggtgaagg aactccggaa
    12601 tcgcacagga taccgtgggg tcttgcgctg ctccatcaga acctcgaggc gcgggtgcat
    12661 tgctagccct taatattccc aatcggacgg agttctgcca ccttttttgt aattcctgca
    12721 cgatcaactg cctcgacaac ctcagaaata ttcttatagg cgaatcccgc ttcttccgcg
    12781 agtcccgaca tcgagacagc tttgacgaga atccctcgct gcttcatttg ctgctgcaac
    12841 tgctcccctc tcaccgattt cttcgcctgg gctcgtgaca ttgtccgccc ggaaccatgc
    12901 atcgtcgagc cgaaggtatc ccgcaatgcc tggtccgtcc cgaccagcaa gtacgatccc
    12961 gtctccatcg accctccgca aatcactggt tggccgattt tcttaaagtc ctgaggcaat
    13021 tccggactag ctggcccgaa ggctctcgtc gcccctttgc gatgcacgac cagttctcct
    13081 tcggaatacc gctccacctt cgcaatgtta tgcgcgacgt cgtagaccag ctccatcccc
    13141 aactcctcgg ccgacttccc gaacaccgta gcaaaagcct cccgaatctg atgggtgatg
    13201 acctggcgat tggcgaaagc ggtattcgcc gcacaattca tcgccgcgaa atagtcctgt
    13261 ccttccgggg aacgaaacgg tgcgcaggcc agctgctgat ccttgaccgc aataccgtag
    13321 cgccgcatgg ccttctcaaa tactttaagg tagtcgcttg ccacctgatg accgaatcct
    13381 ctcgacccgc aatggaccat gacaacgatt tggtcctgtc caatgatccc taatgccgcc
    13441 gccgttacag gatcgaaaat ccgatcgttc gacactactt gaacttcgag atagtgattc
    13501 ccggagccta acgttccaag ctgattgatc cctcgcccca ccgcatgatc ggtgactttc
    13561 gaaggatcgg cgccccccat gcatccctgt tcttcgatat gtacaagatc gcgatgccac
    13621 ccgtagccct cctcgataca ccaacgcgct cccttagtca tgactcgatc aaaagcctcc
    13681 cgatcaagcc tgacgaaccc tcccgccccc actcctgccg gcaccctgcg gaacagctcg
    13741 gtcatgagcc gttccaatcg cggctgcaca tcgtccagcg tgagatccgt tcgaatcagc
    13801 cgcatcccgc aattgacgtc gtagccgacg ccaccgggtg aaatcactcc gtcctgcaca
    13861 tcgaacgccg ccactccgcc gatgggaaaa ccgtagcccc aatgaccatc cggcatacag
    13921 agagcatagc gttgaatccc cggcagacaa gccacattcg tgacttgatc gaagaccccg
    13981 cgatccatcg actgcagaat agtcggcgtc gcatagatcc gcgccggcac gagcatgccg
    14041 gacttttcag acgtaggaat ttcccatatc tcattggtaa tccgattcac cttcatgtcg
    14101 gtattcagtt tcatacgtcg agtaccaccg ttgctttcca ttgattcccg tcctgcttca
    14161 attcgtacag atgcttggta atgcccttca catcgttccg cagttcctgc ctctgttgat
    14221 cgaccggtgc tccgacaaga cgagcctgca acgtccagat tccggaggtc aaggaaaccg
    14281 tcagcggagc ttctcgaaac acgacacctg cagcatcctt ccaatagacg atttctgata
    14341 accattcaaa gagcagattg gatagatcct ggtccaacag ggtaatggcc cgctcccagg
    14401 acgctcccac ggtggcaggg ttggccaagg tctcaaggat cgcctgggtc gcacccgcaa
    14461 agacgtcagg gagagaatcc ccgagcacct caaacgcaac atcggcaagc gcaatatcgt
    14521 cgagatagcg ataggtgaat gccatagaga agcaggtgaa ccagaaacga ccggatcact
    14581 gcaagcggga aggggcgaga atcaacctaa ctcaaacatc ttggggaaat tcgtgagatt
    14641 gcgacaacca tccctcgtca cgagcaccat gtcttcgatg cgcacggcgc cgagccccgg
    14701 gtagtacagg ccaggctcta ccgtgacgac gtgtccctcc tggagcaatg aaccggtccg
    14761 gctgatgcgc ggggcttcgt gaatatcgag accgacgccg tgccccgtcc catgaaaata
    14821 tccctgcatc cggccattga ccagaccggt cttgtacccg gccttctcga accgttcgca
    14881 gatgccgcga tgaatcttca tgccgtcggc gccatccttg atcttggtga tcgcctcttc
    14941 ctgagcatct ttcacgatct ggtacaactt ctttaggtct tcgctcggcc gaccgcgaac
    15001 gaccgtgcgc gacatatcgg cgaaatagcg ggaggtggcc gaccggggga acacgtcaaa
    15061 gatgatgctg cgatgcgccg gcaggggccc gctgccttcg tgatgggggt cgcatgcctg
    15121 ctcccctcct gcgacgatcg tatgttgagc cacgcagtcg cgctccatca acttcacatt
    15181 gatcaacttc ttgatccgct cggaggtgag caccgagcca tcgacccaga gggaatcgtc
    15241 cttgatcgac gcctgtcgca gcagatcatg cgccgccacg acggcctcct cagtcgcccg
    15301 ttgcgcggcc tcaataagcc gcacttcttc ggcggttttc acgacacgct gctcgtagaa
    15361 cgggtccggc ttcgcgtgca cctgatagcc cagttcttgg agccggctgg catggctgaa
    15421 cggaaaattc gccggcacaa gcacgtgctt gatcttcgcg tctcgcaaca cgacatggat
    15481 gatatccacg ctccccggcg tggcgacccc gtgatccctg gcccggcgct caatctccga
    15541 ataagacagc acacgatcca ccttggcttg gctcttggcc cgatccattt ccagatcatt
    15601 catgacaaga aggcgttccc ccttaatctc gagatagata aagggatccg gcgcaataaa
    15661 cttggtcgca taatacaaat tggaatcaag ctcgctggca gcgatgaaga cggtggcttt
    15721 gtgggattgg gatgctgatc gtgtcatgat cggtcggtat ttaaactatc atatgaagga
    15781 tattgattcc caactccccc tcttattcta gcaggatgcg caaaatggcc atccgccaag
    15841 gccacagcaa cggaagagag gaatcgtact gctttccgtc cggtgagtct ctgagcgagg
    15901 gagaacgaag ctggcggact tttccgtatc ctctcgtcgc ggaatctagc acgcggttgg
    15961 gggtcggtca aggatcgagc agcctcaggg cgtcgccgga accggctcag catccggaat
    16021 cgcgtccttc gggaccgtct tattttcgtc cggtgtgacc aagtcgatcg gcagcatgac
    16081 tgtatttttc aacacatcaa aggccagctg ggctaaacca gtgagcccag tggcaaaaga
    16141 cttcattggc agataggtga cttcgggatc ttcaatcgag cccttcacag aaaacatcgc
    16201 ggttgcaaac cccttccgct ctcccgcgaa gagcctcccg aacagtggaa tcgtcttgag
    16261 aaattgcgag tacgatccga agggactgac cgcccatacc atatccacct ggtctgtcgg
    16321 gagatcgtag ttaccggcag cggtaatctt tacgatcgga ctgtcgatga tcagattttc
    16381 cgtttcgaat acgccgttat gtacgttcac cgacgctgta attttattgt agggcaatcc
    16441 ctccttctcc aaatcgacct ttccctgaag cacggccggc agattgagaa tactgatgat
    16501 cttccagatc gcgcgttctt gcgacttgaa gacacggccg ttttccaaca gcacgtcggc
    16561 cttgccgttg agcgacggga aaagcccgtg cgggttacga ccatgccctc gcatcgttcc
    16621 agtcacccgt gcttcgcccg tgatccctcc gtctttcaca ccgacgagct taaacatgtc
    16681 ctcaacgagt aatccggtcg cgcggacgga gatctcgccg tcggccggct gcccctgagg
    16741 caattggacg accagccgtc ctgcaatgtc gccatttgtg gattgtcctg ataaccgatc
    16801 gacatcgagc acgccgtcct ggatcgtgac gcgggccgag agtcctccga atttgaggtg
    16861 cttgtaatgg cccctggcga tcgaagcggt ggcgttgacc ttgcttgttg ccgccagcat
    16921 ttcgagaaag tcacgaatcg gagatcgggc ccccttggga atcaagagat caatatccat
    16981 ttgattggag tcgatttttg ccacgatggt cggcttggct gtccaattct tgatcgtccc
    17041 ttcaagcgtg acgtcactgt ccagcaaccg gaatgataat cgcttgagtt ccgccgcgtc
    17101 cctggccagc agcaaccgca cgtagagatc ctggatctgg ccgtcaacgc ccttcgcgtt
    17161 catcaacccg ttactgagcg cgagccagcc ggtcgttttc catgtgcgcc agtctcgatc
    17221 tttgcctttg acgtccagtg agagttcgag attgcccgct tcaaatcccc ccttggcgat
    17281 ccattccggt accgtggaca atgccacggt gccggtcgcg agcgccgcat cgatggagaa
    17341 cttttcaccg agttgaatct tccccttgat cgggagtcga atggaaggca cggtgacctc
    17401 cacgcgcgtc aacgtgacgc ccttggacga aggcacgtca ccttcgaaca cgatcgtcgc
    17461 aggggcgccg acaggcttct ccatcatgcc gggaagcgtc acttttgact cggttaagac
    17521 caactctcca cgcgcatggg gcgcttccgt tgagccggtg agcgccatga tcccagtggc
    17581 caatccctca acgacgccgg gaggaatcgc cttccccggc aagagttgtg tcatatggat
    17641 ggcatcgccg ctgacacgca cgagaaaatc ctgaaacgaa ctcccagttc ctccggtgat
    17701 tcccccctgc acctgcaccg tcagatcgcc cagatgaccg gtcacctgat cgaactgcgt
    17761 cccgccctcg gcaagcacaa atcggccctg caatgccgtc agtcgttccg gcaaaaagga
    17821 gttggtcaag ttcacgtgct gcgcacgaat ttctcctccg gcgaacgtga cgccggtccc
    17881 cggactcaaa ggcccgacga ggcgaaatgt cagagccgcc gagccttcga cgtcgcgcga
    17941 tgccgccagc acgcgtaaga gctgatcgga attcacggtc ctggagagaa actgcaacag
    18001 atccatcgca gccatggtcc cggaaatgtc catttcaagc catggaccgg cttctaagaa
    18061 cgagatgaga cctttgctgt cggccatatg aatagccccg tacagacccg caaggctggt
    18121 gacccgaaca cggccggcct cgacaaacac gaccgcggac agattcctgg ccggtatatg
    18181 atcctctccg accagcgcct ggccctccgt tacacgaaat tcgcctgtga ccgataactg
    18241 cggaccctcg gcataggacc ccgtgaccgt tgccgacgcc acttcgacct tcccgttgat
    18301 ccggcgatcc tgcatgaccc ccggcagttg cggatgaagc cacgcgggag gaattttcgc
    18361 aagcagctcg tcgacctgta caggggatga ggcaaatgtg acggcgaaag tcggttgcgg
    18421 tgtcaggaga ccggacagac ttgcattgcc gttgacggtc agcttctcca tgttcgccga
    18481 gagatcggac agcagcatat cgtagcccgc cacacccgga atcacccgaa tcgagctgcg
    18541 cgcgttcacg acaccttgca gcagatccgg aaccggacgg gggccgacaa ggtccgccat
    18601 gtctctgagc gcaagattgg ccaattccac atggccgtcg aattgcagcg ccaactcagg
    18661 ccgcgaggag tcttctgcag ccaacgtctg ttgctcggct ttcttaatga cacccgacac
    18721 cgagatcgca gcaggtccat gggccccgct gtagccggct gagaaatgaa ggtcggcctg
    18781 cgctcgttcc gttcgaatta tgagcgcagc ctcgactgtt tccaatttta acgtgcggac
    18841 accatccggt cttgcctcgt cgatcacgat cacggtgccg ttcaccaatg tcgcttctcg
    18901 gattcggaaa attctcccca gcagctgaac cgcttctttg ttgttcgccg gtaatggatt
    18961 cgcccggtca agaacgttcc aatggccggc tcgatcacgc ctcagcgtga gcgtcggtcc
    19021 ctcgatcaac agtcgtttcc ccactacttg cttgcgcagc aatgggatca gtcgcaaaac
    19081 gagatccagt ttcttggctg agaggagcac ctgatcggag ttacggtcgt gaatcgcaac
    19141 gtcggttaac tccagccgga ttctagggaa cagcacgaat ttgatgcggc ggacgtcgat
    19201 cttccgtccc agattctgtt cgagttgctg gaggaaaaaa tctttcagat agtctgcgcc
    19261 ggtcaggtag ggagaaaacc agaggaaagc catgccgccg atcaacagaa tggcaataag
    19321 ggcaacaacg acacgagctg aggacactca atatctccac ttcggaatac ggcgatgcac
    19381 aggctgttca ataggcttgc cagcaaggcc tcagcgagcg aatggccgtg gctcaccatg
    19441 ttgtacgtcg agggtctgat cgacgcgaga acaaagctgg atgtcgtttt cgacagcctg
    19501 aagctcatct tacccaatca cactgatgaa agctagcaag cccgcgaacc aacagggatt
    19561 tttcagtcct tgccttcact cgttcctctg gtgaaggcta ccgagacccg ctataatccc
    19621 ccaccttctt tgggggagcg gatagcacgg cttcacgtga tcaggcaact caccggcatc
    19681 acatcaatcc gctggctcat cctcgcgctg ctctttctga ccagcgtggt cacttacatc
    19741 gaccgtgtga acatctcggt caccgcgcgt cacatgatgc cggctttcgg tttcacgaac
    19801 cagcagatgg gttgggtctt ctccgccttt gtggcaggct atgccctctt tcaaattccc
    19861 ggcgggtggc tcgcggatcg atggggagcc cgcgccgtgt taaccggcgc attgatctgg
    19921 tggtcactct gcacggcgtt caccgctctt gtggcgacgt ccccactcgc cgatgtgctt
    19981 ggactcgtcg gcgccttgat cctcgtgcgc ttcgcgctcg gcgtcggcga ggctgtcgcc
    20041 ctcccctgct ttaatcgcgc ggtcgccaac tggatgccgg ccgatgcgcg cggcgtcggc
    20101 atcggcatcg caatcggcgg catcggcctc gggtccgcga tgaccccgcc tctcgccgcc
    20161 tgggtcatgg tgaattgggg ttggcaaacc gttttttacc tgtcgacgat cgtgggattg
    20221 atcatgggat tgctatggtg gaccctctcc cgcgacaacc cggcgcaaca tccctgggtg
    20281 cagggcaaag atcgagcgtt gattgaaacc agccaggtgg cgacaccgac taccgtccct
    20341 tggtatgcgc tacgaaacac tcggaccatt tggtggcttg tcctcagcta cagttgtctc
    20401 ggatatgtgg cgtatctcta cctgtcctgg ttttatctgt acctcgtgaa cgtcagaggt
    20461 ttcagcgtgt tgcagggagg cctgtacgcg tcgacaccct ttcttgccat gctgatcgga
    20521 tgtccgttgg gcggctgggt cacggatcga ctcgcaacac aacacggcat tacggcaggc
    20581 cgggcgatgg ctgggacgac gggaatgacg ctcgccggct gcgccgtcgc cataggcgga
    20641 tttatcgatt cgcctgtcat tggcattgcc agtctctcca tcggcgccgg ttgtctctac
    20701 tttgccgtgg gagcctactg ggcctcgacc accgatctct cgaaaaccca cgccgggacg
    20761 ttgtcaggca tcatgaatat gggcgccaat atcggcggag ctatctcacc aagcctgaca
    20821 ccctggatcg ccgcacgatg gggctggccg gtgtcgctcg ccacagccgg agccgtcgcg
    20881 ctagtgggag cgctgctgtg gctttggatc aagccaggag aagggttgaa gtcatagcga
    20941 cccttgcaga tcagtttagg aggttgctac agtggacgcc atgagcttgg agtttaagaa
    21001 aatcgacccg cgcgtcacga tcatgacgaa gttcggcgaa atcaagattc gcttttatcc
    21061 ggacgacgct ccgcggcacg tggagaattt tatcaatctg ataaaaatgg gcttttacaa
    21121 cggcacgaca ttccaccgtg tcgttcccgg atttctcatt caggggggcg accccttgag
    21181 caagaacccg gaccgttcgc tgcacggcac cggcagcccg ggctactttc tcacccccga
    21241 aaccagcgat cgtccacaca aacgaggcgc gatcggcatg gccaaggtgc cgcgcgagag
    21301 caacagcacg cgcgacttca atgacaacgg atcccagttc tttatcagcg tgaacgacaa
    21361 cagcgggctg gaccgaacct acaccgtctt cggcgaggtc tttcgcgggc tggatgtggt
    21421 ggacaagatc gtcgccgctc cccgcgacga gcgcgacaat ccgctggagc cgatcacgct
    21481 gacgatgacg gtgaaggagt agcagctaca acggcatcac cccagacgat tgaactggag
    21541 ggatggagag tcaagcctta tcccggggag cacagtccgt cagatcttca cgagttatga
    21601 actcagcgga agcagccgag ccgagaaggg tgctagtcgt cttcggcttt tttcagcctg
    21661 cgatcgagtg caaaccgcgt gaggccgaga tgcttggcag ccacggtttt gttatagtcc
    21721 gccagacgca gcacttcctc aatgatcgcc cgttcaatat caactagagt ctgctgaccg
    21781 agcctcatct cgactctcag catcgagtct gtccccgtcc ccaccgccac cgccgtctgc
    21841 ttcgcgctct cacggagttc gaccggcaga caactggggg tcaacgttcg gccctggcaa
    21901 aagatcatcg ctcgctcaag aatgttttgc agctcgcgga tattccccgg gtaatgatat
    21961 cgctccagca tcgcacgtgc ttccggatca aaatcaggaa tcggtttgcc gaattcttgt
    22021 gcgaatcgaa caatcgcgcg ttgggacagt ggcacgatgt cctcgacccg tttccgcagc
    22081 ggcggcagtt caaatgccac aacattcaga cggaaataga gatcctcgcg aaacatcccg
    22141 cgtccgactt ctttcttgag atcccgattc gttgccgtaa tgaggcggaa atcaacgccg
    22201 atatcgtcgg tcccaccgag acgcctgaag gaacgctcct gaatcacgcg cagtagcttg
    22261 gcctgcatcg actgatcgag gtcaccgatc tcgtccagga acagcgtgcc gccttccgct
    22321 ttttctaaga gtccgaactt ccgctgattc gctccggtaa acgccccacg ctcgaaacca
    22381 aacaattcgc tctcgaacaa gtctttcgga attgccgtgc aattgacgcc gacaaacgga
    22441 ctcgatgccc gtgctccatt gtggtggatg acacgcgcga gaaactcctt tcccgtcccc
    22501 gtttcaccga gtagcatcac cgacgatttc gggttctcgg ccacctcgcg gacttgttcg
    22561 agcaattgct tcatcacatg actgtgggct tccagattgc tcaactggta ctggctgtcc
    22621 tggtgctcat gctccgactc aagacgccgc cggaggctca ggacatccag ggcgcgaccc
    22681 aacaccggct caatcccctc cagatcaacg gatttgatca cgaaatcgta tgcccccaat
    22741 ttcatcgcct ccaccgcatc ctgtaccgtc ccgtacgcgg tcagcataat caccatggcg
    22801 gtgggagcca cctggcgcac ctgcttcagg gcgtcaagac cgctcatgcc gggcattttc
    22861 aaatcgagca gcacaagatc tggcagatcg cgctggagtg cttccagaag cgcttcaccg
    22921 gactcatacg ccacaacgcg atggccttgg cgactcagcc gtttagcaag ggccgaacga
    22981 atcgccagtt catcatctgt cacaaagatc acggcgcgca tggatcagct aatccccttc
    23041 agatcttgac tcaagggcag ccagattcga acagtggtcc ctttgccgac ttcgctggtc
    23101 atgtgaatct cacctgcgtg gctgtcaatg atattccggc agatggccaa tcccaatcct
    23161 gttccatgga ccttccccgt tgtaaagaac ggctcgaaga cgtgcgagag agcgtcggct
    23221 gagatgccga ccccggcatc ggacaccttg acggtcaagc ccggacattc attgcgagga
    23281 agttcatagg ccgttacttc gatggcctgt ccccccgcag acgacgcgtc aatggcattg
    23341 tgcagaatat tcaggaggac ctgcttgatc tggtctcgat ccgcgtgcag gagactcagc
    23401 gtgggcgaga gggaacgttt caccatcaga cacttcgcct gcagcggcgt ctcaagaagt
    23461 ttcaccacct catccacaag ttcctgcaac gcgaaccgaa tcggcgcaag gtttcgagga
    23521 cgagcatatt caacgatctg attcacaatc cggtccaatc gcttggtttc cttcaggata
    23581 atgtcgatat cgtcccgtct cggatccttt tccgagaatt cgtccaacaa taaggcagcc
    23641 gtcgagccga tccccaccag gggatttctg atctcatggg caatgccggc ggcaacttgg
    23701 cccaaggtgg cgagtttctc tgaccggctg agtcgagatt gaagatcgtc taacgcggtg
    23761 atgtcgatat tgaaggccac gtataccggc aaaccatctt caccgttttt tatgggtaga
    23821 cggcgactca gaatcttgcg caccgcacca tcctcgtgaa ggaaacggaa aacggtttcg
    23881 taaggctgcg catccgagac ggcacgtgca aactcggaca agacgcgctc ccgatcttca
    23941 ggatgaaacc gatcacggaa cctggcggga tccatcggtt catcaggatc gaacccggca
    24001 agcgcctgat tatatcgatt gctgaaggtg acctccatgc ctttggtggt aaagattccg
    24061 aagggagcat gatccaccag gccgcggtat ttggcctcag aggccgcgag atgctcattc
    24121 agatggcgaa gagccgattc ggatctctcc atctcatcca gatgtccgcg aatctgcaat
    24181 cccatctggt gcataaccct tgtgagatcc ccgatttcgt ctttgcgtgc cagaacgtcg
    24241 atggcgggga ctagcccaag aggcgacgat cctactgtct tagagaggct gacgagaggg
    24301 acggcaatcg atcgtgcaat caggtataac gcgctcacga tcagaccaaa ggtgagcact
    24361 ccgccgccta aaaccacgaa cagcgtcgag gagcggtcct ggctcaattg cgccaactct
    24421 tcacccactc gttgctgttc gagttggtca aatcggacca tccggtctcg gatctcgacc
    24481 atcagcgctc gtccccgccc ttcttcgata tactgcagcg cgtttcgctt acggcccaac
    24541 ttgatatcct tgatgagttc ttccttctct gcgatcaact gagtcacaag cacctgaatg
    24601 tcgttgaatt gttgtagcgc ttctccggaa atcctgtgct tgagatccgc tgcgatgagc
    24661 agaatgccct cctgtgccac tcgatacggc cggagatagc gatcttgctc ggtcaataca
    24721 tatcctcgaa agccggtctc cagatcgaca accagacgca tatactgagc tgcgatcttt
    24781 tcgctgagat acagattatt gaggtgctcc tcgtcctggg aaaacttctg cacactgcta
    24841 tacgtcaaca cactaagcag aacgagggcc gcaagtggaa tagcggaaat gaatagaaac
    24901 ttgcgacgaa tcggcagatt atcaaacagg ctcgcgacat gctgtcgcat gggtttcctc
    24961 tcttaatcga tcgcacatcg taggattccg acccgttgcc tgtcaaacct gactcccgtc
    25021 gcattgattc gccgccatcc actcgatatg gatgcccgag aggcgcaaat cagcacgaca
    25081 gcctgcgaca tcatcaagga ggctggttcg tctatccggc tcggtattaa cgcaggatat
    25141 cggctcattc cacacagaga gaagaaatgc ggacaccaac ttcagctgtg acacaatggc
    25201 acactcatga caggtcgaca cgagcccagg accgcagtcc aacctgcgat agggaatatt
    25261 gattcgatca cgaccttgtc caagttgatg ccattaccta tggcagagcc gataatggga
    25321 gaagaaggtt tttgtggcac tcgaagtctg ttcagcaata gcaatgccag acacatagcg
    25381 acagagccta tgtattctca atgacgaggc ggcccaggcg ctgcgcgtcc tcaatccact
    25441 gagcgattcc gcacgctgcg caccgtgcga atagattctc tcgcggacat attcctaaac
    25501 ccgatagctg tttatgcggc cccacaaacc tttccttctg gaagccttca gcttttccct
    25561 gtttatcgtc ggccgcgctc cgccggcgat gaggactaag aattgcacaa tatgtgcgta
    25621 agacgatctc cttcgctatc cacatcaaga ccagtacagc gaccggcagc gtattgtgaa
    25681 catagcggtg tatcgcagag acagggggag gcggtcatga cgtcacaaga catgtcaggc
    25741 gctgaattcc agccatcaat cgacagacag atattgaagc ttcagcgaag ccgccggcgt
    25801 gtcatgttct ccgaactcgc caatgctctc cccgactaca cgtggcatat gttgttcatc
    25861 gcccttggcc gattacagaa gcgacactgc gtcgacctta tggcacacca atgggactat
    25921 gagatcattt ttcttgagga cacttcccgc aagcaatcgc tagcccgttc gcctcaacaa
    25981 ggtaatcaga gcgaccgcca tgagaggacg cacgcgtaaa tgggctgtgg tcttggtctt
    26041 gtcactggca ttttccgccc tcggatgcat ccgggcatct ggccccctcg acctcacgtc
    26101 acagaatccc tactacgatc aagaacaaat cgccagttat tacactaggg aagccaacaa
    26161 cttcaaactc aaagcacagg agctctcaca gcgtgcgtcc gtttacgaag gactgttcgg
    26221 ctctgattct gaatgggtca agggcacacg gttgttagag cagttttatg agtactcggc
    26281 acaggaacaa gaacgtctcg cggccgaaca tctggacatt gccggccgtg aacggcaatc
    26341 cctgatggta aggcccgccg gcccctaatt gcggtcactc ccaatgaccg aaggaggtct
    26401 ttatgtctcg cctcatccgc catagcttga tcgttctcct gccccttttc cttctgttga
    26461 gcaccgcctg tgcgggaaca agtgtccagc ccggccagcg tggactaatg tggcatccct
    26521 tctctgaagg gctttccaca gaaccgctca aggacgggta ttattggagg gccccgtgga
    26581 acgacatcta tgtgtatgac gttcggtggc aaagttttac cgaaaacgtt gacgcactca
    26641 gcgccgacga ccttcaagtg ctcgtgaaag cggcgatcat cctgaggcct attccggaag
    26701 aagtatactt cctggcacac gagctgggat cggatttcta cccacgcgtc gtcaagcctg
    26761 aatttttggc cgcggtacgc agcgtggtct ccggctatag catggtgact gttccagaaa
    26821 agagcgctga gatcgccagt aaagttcaag ccgtagtggt cgaaaagctg aaagggcgac
    26881 accttgaaat tcaaagcgtg gcgctggcgg acgtcgattt tccacaagtc gtgttgagcg
    26941 cgatcgagca aaaacaggcg aaagaacaag aaaaagagca aaaagaattt gagttgacga
    27001 tcgcggctaa ggacgccgag attgccagaa ccagagcgaa gggcgagggc gacgcgattc
    27061 gcattcgggc ggacggagaa gcggaaggct tgcggatccg tgcaatcggc caggcgaaag
    27121 cacaagaaac cattacgaag acgctcacac cggactatct gcgatacaaa ttgtacgaca
    27181 gcccgaactc aaagctggtt ctcctgccgg acaagttgaa cgttcctctt ctcgtcaatc
    27241 ctggagccga ccagccgcat cagcttctcc cggccgagtc ccatgcgcgc tgacgagctc
    27301 accggctgct gaatgatttc ttgagtatct ctcgggaggc cctggacagt gttagcgggt
    27361 tcgctggcct gtggacatac cggtaggacg ctggtcgtgg gtaccattat ggcctaccgg
    27421 ataaacgcat acctgcggga acactcaaga gaaatcaccg ctcgtatcga caaggcggga
    27481 ttccccgatt gattccacat ggcatcagct gtatgcggtc tacaatcaaa gctagtttcg
    27541 ctctctcgaa tgtgtttcaa taaagggcgc gctgcacttc ctgcagggcg aacctctatg
    27601 atcaggaaat cgtttaaggg tttactgtcg ttggtgattg tcacggccat cgcgctgatc
    27661 gttctgacga ttgccacggt taatctggtt gaccgctcgg tcatcgaaga cgcggctgca
    27721 gaggaccgtc acagggcatt cctactcgcg gctggtgctg tgagccgaat tatttctcag
    27781 tcgggagata tccacaatgt gaacgcactt gaagccgcca tccatgatgt cttgacactt
    27841 cgacccggtc tccgccgatt aagcgtgttt gatgccaggc aaaccaacgg ggcgctcatt
    27901 tggagcagcg attcagaacg ggcgccgaaa tcgttgaccc ctcatgaact cacggaagtc
    27961 agggcaggac gttcagccag ctacttcgac acgtctacga atgaccgcgc ctgggtcatc
    28021 gcagcgccga ttatgatgaa cggtcagacc atcggggcgc tgcgcggcca cttttcggtg
    28081 gctaaattcg atcgcattgc cgaagaggag caaacacagg cgggactcat tgcaatcgcg
    28141 atggtcgctc tgacctgttt ggcgttcttt ttattgattc aacgccaagt gcacagaccg
    28201 attcgccggc tcctggcagc gatgcatcgg gctgaagcag gagacctgac aggcggggtt
    28261 tcgcttatag gcccgagaga tcttcgagag gtctcgcggc aattcaatca gatgctggac
    28321 cgagttcgag cggcgatgga tgacaaagaa aaactgctcg aggaaataaa ggactttaac
    28381 gggaccttga cgacgagaat tggagaggcc aagcaggaac tccaacagac tcatgccatg
    28441 ctggtcgagg cccgcatcca ggcggagcgg aatgagaagt tggctgcgct cggagaatta
    28501 tctgcggtca tggcacatga actcggcaat cctctgaatt cgatttccgg gcacctccag
    28561 ttagcactga aggaggggcc gcaacaagag catcatcgcc acctgacgat cattcgttcc
    28621 gaaatcgatc ggatggtaac cattatccag catattcttg agtcgacgcg actacacgtg
    28681 caatcggctt cggtcgatct caacaagatc attcgtgaca ttcaaagctt aatcgccccg
    28741 ggactctcag agaaacggat cgtgttcaag acggatctcg ccgaaccgct ccctcctgtc
    28801 gccggagatc agcgatcgct ccacggtatg atctttaatc ttgtgatgaa tgccgttcaa
    28861 gccatgccac atggcggaga attgggggtg aaaaccctcc gggccattga ccatcaggag
    28921 gagggaactc tcgtcctgcg cggggacacg gcattgaaga agggagccgt gcgactgatg
    28981 atcagtgaca ccggccatgg catcccctcc gatcgattga ccaaaatatt tgaaccgttt
    29041 ttcaccacac ggcaaaacgc aggaggcacc ggcttggggc tggcgatctg ccatcgagtc
    29101 gtctcctcag ttggtggacg aatagccgta cgcagcgccg tggatgaggg taccagtttc
    29161 accgtggact taccgatatg gaaagaacct cgagggggaa aaccacacca tgaatcgtga
    29221 cggctgcatc ctcatcgtgg acgatgacca acgaaaccgg gagatgttgg cggaggccct
    29281 tcagcaggcc aacttcaatg ttgaactggc cgccagcggt gaggaggcca tcgacaaggg
    29341 cgggcgtcgc gaatatgacg cggtcttcag cgacatcaag atgacctcgg tatccggcat
    29401 ggaggtgttg gacgcgttcc gaaccactgc gcccgaaacc ccggtcgtgc tgatgaccgc
    29461 gtttgggtcc gtggagaccg cgatccaggc catgaaacag ggcgcgttcg attacatcac
    29521 caagccggtg aatctcgaag agctgatctt ggtcgctcgt cgggctgtcg aacattgtcg
    29581 tcttgtccgc gaccatcgcc atctgcaaca cgccttcagc gaacgaccgc gcgccgcgtc
    29641 catcatcggg caaagtcgga agatggtgga ggtgtttaag atcgtcggca aggtcgcccg
    29701 cagccggacg gcggtgttga ttcagggaga aagcggcacg ggaaaggaac tgattgcccg
    29761 tgcgatccat gacaacagtc cccgtgccgc acagccgttt gtcgcggtca attgcagcgc
    29821 catcccggac tcgcttctcg aaagtgaatt gttcggccac gtcaagggct cctttaccgg
    29881 agcccatgcc ttccggcgcg gattgctgga agaatccagc ggcgggacct tcttcctgga
    29941 cgaggtcgga gacctctctc ctgccggaca agccaaactg ctgcgggctc ttcaagaggg
    30001 agagatcagg agagtcggaa gcaatgaggc ggtgcccgtc gatcttcgtg tgatcgcggc
    30061 ctcgcgccgc gaccttcatc agcttgttaa ggcagggcac ttccgggagg atctgctcta
    30121 tcgtctgaat acagttacca tcacactgcc tccactcaga gaaagaatag aggatatccc
    30181 gcttctcgct gagttctttc tggcccagta tagcgccgag aaacaggcga ccactgcatc
    30241 cctgtctgcc aacgcgaggc acgctctact ccgctacccc tggcccggca atgtgcggga
    30301 actggcccat gtgatggaac gggctgtggc gctggcttcc catagcgtgt tgtcccttga
    30361 tgatcttcct ccggaaattc gcgacagcgg gagcggtcaa agggatctga tgaaaggcct
    30421 gccaggtacc ctgagcgcac tgcagcgtga gcatgtcctc aaagccttgg attcgacaag
    30481 gggtaataaa gaaaaggcgg ctcgcatgct gggcatcagc cggcggacgt tgtaccgatt
    30541 tctcgatcgg tacggtttac acagaactcc accgtcatcg gacgccgaga ccctcgactc
    30601 gactgcgtca tagtcttttc tccagccatc cttacccgag ctaacttctg cttatcatgt
    30661 gccgccgtgg catgccttgc catattgaca caacctacga cgcagggttg agcggtcaat
    30721 ccttccgcaa gtctgttgtt ctgcccactc gttgttcccg accgctcaag cacccacagg
    30781 gctggcatca ctattgctga tcattatttt cgtgaaattc tatgaacgtc ggaatgaagc
    30841 cggcggtgag cgggaggaca accacacgca tggcacaccg ggaggtggca atatggcatg
    30901 cgggcgatgc ggcggtttcc tcatagtgga aaggggttac gaaaaggctg ccgtcgtaac
    30961 gcgaaccggc atggtcacca cacgatgtgt caattgcgga aacctggaag atcctttgat
    31021 tcgatcgaat cgggccgtgc gatttctgtc tgatcggaca gtgcgaaaag ctgaagaaat
    31081 cggcgaagca acacccgacc ctgtcatacg gcaatcatgg taaagggagg ttgtcatgga
    31141 aggctacggc aaacgagtcc tcgtcgtgga tgacgacgaa cataccagac ttctcatggg
    31201 caccctgctt gagggagctg gctacaatgt cgtggcggct tgtgacggcc ttgacgcgtt
    31261 ggtcgagatc aagaagcggc attttgacgt ggtcatcaca gacttatata tgcccaacct
    31321 caatggtatc gagctgcttg gtcgtattaa tggcatacac cccgagattc cagtgattct
    31381 cgcctccggc tgtctttctg agttcctggg tacacgaaaa gcctcccaat tcttcgcgtg
    31441 ccttcggaag cctttcgaga acgccttttt tcttgagatt gttcgagtgg ccgcccacgt
    31501 gtcagataaa acccgcgctg aaggagtgtc ggcttggcaa tgaagcagta tcgagccacg
    31561 cactgttcca ggttggatgt tccggatctc ttgctgaaag atgctctggc atgtggtgtc
    31621 cggaacacga ttcgtattaa acgcagagtt tccccggagg aacagtgcgg cccgtcatgc
    31681 atctgaattc gtagacatca tgcggccaac ctgtgaagag cgacaacaac acgacccagg
    31741 ccatgcttgt gaggatcgac agtcactgca aacattgtct gagaccgtgc agaaactctt
    31801 cacagtgtag ccaactcgct tctcgaactt cgaagagaac aacgggtaag aggtgactca
    31861 tggaactatc gcggcccccc cagtccatca taagaacaat gttgtgtgct tccataatcg
    31921 ggtcggtggc agccgtccca gacccggcga tgggatctca cgaacaggct gaaccggaag
    31981 aaagacgcac cgtcggaatt tttgaaaaat tggctcctgc aaccttattt ctttctgtca
    32041 catacgattc tgcccatcct ttggcatccc tgacacaaat gggggtgggc gccggtttca
    32101 ttgtagatga tacaggcagg gcattgacga acgcccatgt cgtagacggt gcgtccacga
    32161 tcaccgccac gctctacgac ggtcacaaag tcaaagccga actgctggga tgggacccaa
    32221 tctcggatgt ggcggtgctt caactcgaaa aacatgacag ccccttcctc ccagtcaagt
    32281 tgggagactc aagccaactt cgcgtcggtc agcacgtctt ggtcgtcggc aatccgttcg
    32341 gacttggatt taccctcacg agcgggatca tcagcggact tggtcctgga acgtcggtga
    32401 tcgaatcaac gaacaaacgc ctcgtccaga cgacggcccc catcaacccc ggcaacagcg
    32461 gcggtcccct ggtggattcg cacggaagcg tgatcggcat ggcgacggcg acgctgatgg
    32521 gagcacagaa catcgggttc gccatcccca tcaacgtcgc aaaggatgtc ctgactaaac
    32581 tccgtgatca ggggacaatc gagcgaccat ggcttggcat caccggacaa tttgtgacgg
    32641 acgaggtcca gaggctcttt gcccttccac tccccaaggg tatgctggtt gtggatgtcg
    32701 atgacggcgg cccggctgtc gacgccggat tacggtcggg gaccttgcat gtgacaatct
    32761 aagggcaggg atgggttctc ggcggtgaca ttattgtctc ggtaggagga caacgagttc
    32821 caaccgctga ggcgtttctt gcagccatga acgcccttca tgtcggtcag aaggtgccga
    32881 tcgagattgt gcgcgatggc gggcgtcttc aagggtcgat catgattggg gaactcccgg
    32941 tcggtgcttt gaaatcaaag agcgggcccg gccagcaggt ggctggagcg atgttccccg
    33001 gcaccatggc agggccacac tacgccgccg tctctttttg acgatggatc ccattgacca
    33061 gagcgcaatg gctcagaacg cacagtgtga ttttgatctg ctctgtatcg gcagcgggcc
    33121 ggccggccaa cgggcggcag ttcaagccgc caagttcgga aagcgtgtcg ccctcgtgga
    33181 acggaggcgc ctcttggggg gcgtgtgcgt agaaactggt acgatcccga gcaaaacctt
    33241 tcgcgaagcc gtattgtcct tcctgtgtgc tgccaaccac agagaacagc atttaggcaa
    33301 tcacttcgac agccgaccca cggcacatca actcctctcg cgtgttgaag aagtgaccaa
    33361 gcgggaagtc gaggtcgtcg ccgaccaact tcgacgaaac gacgtcgtct tgatacaggg
    33421 ggaggcgtca ttcaaagacc cccataccgt ccttgtggtt aacgaacatg actccagaat
    33481 cgtcactgcg gcaaacatct tgatcgcggc tggaacagtt cccgcttttc ccgacggcgt
    33541 acccgtcgat ggagaggtca tcatgaccag cgatgacgtg gtgaggctga cgcgattgcc
    33601 ccgcaagatg gtggtcgtcg gtggaggcat tatcggcatc gaatacgcgt ccatgctggc
    33661 tgccttgaag atcaacgtta ctcttgtgga caagcgcaca cgactcctgg agttcgtgga
    33721 ttccgaaatc gtcgaagaat tgatgcatca gatgcgcaat cgcaacgtca cgtttcgact
    33781 gggcgaggct gttgaaaagt tggagctgac gcaggaagag cctcgtcggg ccgtgctgat
    33841 tctggagtcc ggaaaacgca ttgtggccga atccgtcctc tattcggtgg ggagagtcgg
    33901 agccgccgac cgcctcaatc tggctgcggc aggtctgaca gcagacaaac gaggccggtt
    33961 gagcgttgac ggtcagtttc gtaccgaggt cccgcacatt tttgccgcgg gcgacattat
    34021 cggctacccg agcctcgcct ccacctcttc atcgcaaggc cgtcacgttg cctgtcatgc
    34081 gttcggagtg gaaatcgacc ccctccccga acatcttccc gtaggcattt acgcgatccc
    34141 cgaactgtcc atggccggac caccggaaca tgtgctgacc gagcagcgca ttccgtacga
    34201 aacaggcgtt gcacggtatc gagagatcgc gagaggacaa atacttggtg atgacagcgg
    34261 tctggtcaaa ctgctctttc atcgggaaga ccgtcgcctc ctcggcgtgc atgcaatcgg
    34321 aaccggtgca acggaactca tccacattgg acaggccgtg ctcgatctga atggggggct
    34381 ggattacttc ttacgtgcag tcttcaacta tccgacgttc gccgagtgct acaaggtagc
    34441 ggcgctggat gcattcaata agctgtcgat gtaatccaga cacgtgaggc cggagcatgg
    34501 agagaagcca gtgcgtgtcc ttctatcaca ggtagtgaag aagcaacgcg ggaatgccgt
    34561 ctttgtcatt gtggcgattc tcggaccgtg tctgctcctc gctgcctgtg cgagcgtaga
    34621 caccattctg ctaaccagcg agaagtttcc tcccaagaag tctgcagacg aagtcgccgt
    34681 gctggagcag aaaccgactc gcccccatgt ggacctcgcg gaactgcgga tcggagatag
    34741 tgggctcagc tttggcagtt tgcaacacaa gatcttggac aaggcggcat cgcttggagc
    34801 cgatgcggtc gtctttgcca agccgcaaac ccagacgacc cacgaggtgg cgtatcaacc
    34861 gctgtatgat ccgtggggct acaacagccc ctactatgga agcccatggg ggtatggcgg
    34921 atacggtggt ccctatggag gatggagccc gtggggggga ggctactccg gcagcatcgc
    34981 tgttccatat gacgagacca tgaatatgct catgggtacg gccatccgtt acattgaagc
    35041 aacaacggct ggtggtctca aggaatcagc agcaacgcag ttgggaaggc aaacacaata
    35101 aataaacaat ctgtggcccc acagtgcaga tcgcaccttc tatccagcat agtgattcag
    35161 gctcgcctat ggtcatcggg tgtgtcgcct tgttgtgttg ttcctgcgcg atgctgaccg
    35221 cgtgcgtatc cgtacgggtc gagcccttga cccatgagtc ctatgccccg cgcgccgatg
    35281 aaacccaggt gcaatggctg gagacagcac ccggttctcc gtacatcaag ctggcgcgga
    35341 ttattgcgac gagtcagagc gcggatgagg accggatgaa ggacaaagtt ctcgcccgtg
    35401 cggccacact gggagcggat gcagttgtgg gagggaagtt cgacatcttg gaatcaatag
    35461 ggtcgggtcc cctctatgaa tccacgttgg ggccggcggc ggacagtttc agcccgtatg
    35521 ccagcggctg ggggtggtgg aacccctttt attacgaccc ctggagtttc gtgcaaggcg
    35581 cggctgatca gactggtcga acagaatatt tatccggcac agccatccga tatgcgaacc
    35641 agaaatagac gcatctggaa ttttgagcag gtcggttgga aagacgacga tgaaacgcat
    35701 tcccgagtcc aggcggaaga gcgcacagat gccgttacaa caactcatca agtcccgcga
    35761 acagcacaat ggtcaacaca atcgaagcca tcggattttc tctgaaggag gctgtatgaa
    35821 aaggcaccgg ctgtggacgc ttgtcggatt cacgcttgcg atgggcatcg catcctgtgc
    35881 cggccccaat cagacgaacc aggaatctac tccgcccgac gatccggtca ttggtctttt
    35941 gagtaaaggc atcacgcaac tcaacgccaa catcaatgcg ctgtcgaagc gcatgaacga
    36001 tgtacggcag gcgtcagcgg gaacggaccc cgtgctgcag gaattgcagg cgttggatct
    36061 gtctggatgg caactgcacc agcaacaatg ggtgttacag cgcaatcatc ttgtcttggc
    36121 gcgtgacctg cttcaacggg cctccaagag ccagggaacg aatggaccgt tgctggacga
    36181 ctggcgcaaa caccggcagg agtacatgca ggccatagag gacctgcgtc aacaacgaca
    36241 gaccttggag aataaacatt tagaggttga agctcggtta gttgagcgcg gacttcagta
    36301 aaccccctac tgcggtctgc atctttgttg atagaaatga gataaggaca accaccagac
    36361 atcacggctc gtaagctcag agagacttac atatggccaa aggaggacaa ccatgaagaa
    36421 acgcgggaat gggaagaatc tcgtcaaggc aagtccagac atgcagtcag agtctctcaa
    36481 cacatggaac aatagaacat gggaccgtat tgcaacaaaa gcatatgaat tgtacgagca
    36541 acgagggcgt atcgatggtt atgcactgga tgattggctg aaagccgaga cgatgataca
    36601 cgggaccacc gcatgagacg tttaccgccg ctaaggcatc ttagactgca tcccgaatat
    36661 agaaggggca gatcttcagc ccgatgatcc cgcacatcga caaaccatac cgacgaagcg
    36721 gaaacagccc aagggagtaa cgtttgaggt gacaggatca gtggaaacgt acgagataca
    36781 ccattctttc gaggggcaaa ctacaactat tcatgaaccg aaaggagaca gtatgaagag
    36841 gctactcgcg ctgttcgggt tcacactgat cagcactatc ggaatcgcaa ctctctccta
    36901 cgcagctggc tccaagctgg ttgagggaga cgtgctgaag attgaggggg agtattacac
    36961 ggtgcatgac acggcaggcc acgacgagcg cctgcatgtc gacaagacca cccatctgga
    37021 gggcactttc aaggttggcg acaaggtcga ggtctatgcg acggataaag gtcacgctcg
    37081 ggctatgtac catctcacac acatacagcc ggctccgtag gcagcgggat acgatgggac
    37141 gctctcactc tggtttgggc accggcgcgt catccgatct ccaagcggcg aaggccccgc
    37201 gccttgctgt gcagcgcgat ggtgaaaccg ccgcccttag aagaaacagc aaatggccaa
    37261 tagacctgca aataagcgtg gtgaaaccaa tttttcgtct gcatttgata tatcgaaggg
    37321 aagacgctct gggataaaca agcgatggga cacgggaatt gaccctgatg gtgcctccgg
    37381 atggattccc gttacctgca catctcgcaa cgataaggga acatctgctt acaattcgcg
    37441 atgctggctg tcacgctgaa atcgctccgg actcaagagc tcgtaagacc caacacagag
    37501 catctttatc atgatttttt caacctgacc acaattgctc ccatcatatc gcctttcttg
    37561 aaatccttct tcacgctgtt cggatggtga ttgtgacaat ccacacagct ccgctcgatg
    37621 gcaaaatcgg cggcaagccc cttaaaatct ttcccgtctg cgaatgtcat tattttacga
    37681 gtcggatctg ccatgagttc cttcaaggcg ttcacctccg tctcggtctt cggaaagttc
    37741 gaactataca acggtgtcag ggacaccagc ccgatttcaa catctttctc aagggttttc
    37801 atttcttgtc ccgccatctt cacgaactga aacgggagca taacggcatg gtcgtccttc
    37861 atccagtctt ctttgggtag aatcccttct ttactcaccc gttccgtgac ttgctcgacg
    37921 tagacggtcc ggaatgcttg gactgtcgca agaatatatt tcacaacaag ttctttagag
    37981 acctccgcta ctccaactag gggagccatc aacaagacta atccaatagc gcccgctaat
    38041 ataatcaccc cctgcttcct gtcctccgcc attcttgcct cctctcctta actgttggag
    38101 tcacttatcg tctacgagca agaccacttg ttcggcagct ttaataaccc gctgtaaaga
    38161 caatcgcaga ccctgggtgt ggcctattga tagctcatcc tcccatcgca ccggctgctc
    38221 tccaccctat cacgttgcaa tgagggttat tgatccctcc tctgtttcaa ctcgtaccga
    38281 caccggtata gcgctggact ggttgcttcg cacagtgcgt cgcgtgcgaa atcgctcaaa
    38341 ccagctgtct cagtaatgaa aatctcgcat tttttgcttc aataccaatg gcacatacct
    38401 tgctggataa acaagggatt cacagctcga tatttgtcac acgactggag aggactgtac
    38461 catgctggct tggctgaaca aatggggaga ccgggaatta ttttgggtca tgctggcact
    38521 tatcgggctg gtcgtcttcg gcctaaacgc cttggcagaa gccgctccgg tcggcccgag
    38581 cgactgggag ttggctacgg tcgtagcagg gagagatacc gctccggaag tcgagcggaa
    38641 gctcggcctg tctccctgcc tggttccgaa ctcgaccggc gacacaatct cctatctata
    38701 caacgtacca ggaacgaaag ggccgaacta tcttcgactt gatgtcaacg gccatgtgga
    38761 tgccatcacg gtatctcagg atcctcctat cgtgggtgtc tgttatgccc ccacgcgcgc
    38821 atcactgcca gtcagaaccg ccaagggaat cgagcttggc gccacaattg atgaggtcat
    38881 gaagctctat ggtgaaccga ctgaaagatt cgccgtgggc cccatggccc ggtttcgcta
    38941 tgtcgccacg tacgaccgat catacgaatg ggatctcgtg ttccggaacg ggcgtcttgt
    39001 ggaatggaca gtctcgaccg aggattagcg gctaatagtc ttgccggtaa cgatatcgaa
    39061 gcagcaacgc ctcagctcgt cgttgagccg gagccatcac cgggagatcc gaataattgc
    39121 ggacaacttc tgaggcaatg ctttcgccca ccgcagtgct cccgtgtttg acgtactcat
    39181 ccgccacatc cattagaagg ttcgttctct gttccagcga ggacacgaga tcgggaggaa
    39241 atggccggtg gacgtcttga taggcccgat ataacgcgaa cccatgatcc agatattcgc
    39301 gcaccgcacc ttcatagctc ttcaacgcgt ctaaggatcc tgcctgctta tacgactcct
    39361 tagccgcgag catgttcccc ttcaatgctg cttcttctcg ctcccatgtg gcgaaacctt
    39421 ccggggtcag tagcgcttcc gccgcaagtc cattatgctg acctcccgca caggcctgga
    39481 gcatcaaccc catgatggca cacagagtgc caacacccag cggtgacctc atgacactcg
    39541 caccgaccat aatggcctcc actgcagaga aaggttctcc gacgatgact gctctatcac
    39601 atttcccagg atcttgccat cacaaccgcc tgatccgatt gtcgtgctcg cttctgttat
    39661 tgggcgctct gtgggtgagc ttcagtccgg atgcgctagc tgtagagccg tctttcggat
    39721 tttacgatga cgttcctcct atcgatcaaa gcgaaatgga cgccatggcg gaacgtacgc
    39781 gtgaagctcg gaaacgactt aaacactcgg agtttgcgac atcaacgcag cctgaatcga
    39841 tcgaacgaac agtcccgctt ccgccaccga cgaaagacgg gcgggatgac atatcggatg
    39901 ttgacctaca agaagttgat gaactccgta aagcgttcca agaaatgcaa tctcaggcca
    39961 caaaagaagg cgcccccgcg tcggagaaat ccgggcctca agagggaccc tcccctcctc
    40021 catcacaacc cgggccaaag aaggaactgg atgatgagat taagtcgtta acagatcgag
    40081 tcaatgcgct ggaacgcgtg ttggtcggca ccgaagagcg gcccggactc gcaggcttga
    40141 ttggaggctg gacgcggcac aacgggttct tcttgatgtc gagcaacggc gattttttcc
    40201 ttcggctccc cgtgttgttt caatcggatt atcgaagttt tcccggcggc caaaacggca
    40261 ctgatccagg cgtacaccca aacaccttcc tcctccagcg cgtccgcccg atggcgcacg
    40321 tacgtgtctg gcgatacttc cacatgctga tcaccccgga ctttggtaat ggcgtcatcg
    40381 atcagaaccc atcccaaaac cagagcgttg tccaaatccc cgacgggttt ggcgaatggg
    40441 attatttctc tgccatgcgg attcgtgtcg gaaagttcaa aagcccgatc gggctggaga
    40501 tgctgcaagg catacagaat ctgagcttca tggagcgttc gttgacacgg aatctccttc
    40561 cgaatcgcga catcggagcg atggtctggg gcacgttctg gcgcggggca ttggaatatc
    40621 aaatcggcgt cttcaacgga gcaccgaatg caaatttcta tcaggacacc aaggccgcga
    40681 gctcggataa gacgatggag gcgcgtgtct tttcccgccc cttcgtggat cactcagtgg
    40741 aatggctcag aggactgggg gtcggagtcg ggatgagtac aggatccgta agcaacacca
    40801 gtggtcagga aatcatgcag actgaaacgc tgagttacac cttcttcaat tacgggtcac
    40861 aggttcaggg cgacggaaac cgtttcaggc ttgcccccca ggccgcgtgg tactacaagc
    40921 gggtcggtct ccttggcgag tacgtcagca gtacccagca tctcaagaac ggcaatgggc
    40981 cggacaggcg gaccacgaac gatgcctgga tggcccaaat gtcttttctc ttaacggacg
    41041 acacctcgac ctacggccac atcgaacccc gccaaccgtt caactggtca aaaaagcaat
    41101 ggggagcctg ggaggtcgcg gctcgctatg cgcaaatcaa cctggatcaa gacaattatg
    41161 cgtttaactt agccgatccc acggtcaatg tgctgcgggc caagagcaca agcatcgggc
    41221 tcaattggta tctcaacagc aacgtccgga ttaccggaaa ctttgtccat accgacttta
    41281 ccggcgcaac cccggcctat cgtgcagcgg atcacgaaaa cggcctgatg tttcgcgctc
    41341 aactgacgtt ctaagccgtg gcaactatct ggctattcct gttcgtcctg gcctgcgcaa
    41401 acgttgcgtc ggctgaggat ccgcttccgt cccagcaggc ggataccgcg ccacccccga
    41461 agacttctgc ggagcccgaa gttgaatcga gtcccgttca aggtccaccg gatcccacca
    41521 aagctcgtca acgtcttcgt gaactcgatc gacgtctcag cgtgctggaa tctgttgcag
    41581 tcgggcgcgg acatggacgc ggcgtgatgg aacgaggact gggttggtca ccagagcagc
    41641 gagagcgcgg tttcgcactc tactcgacgg acggcagcta tcggctgcat ctgaacggag
    41701 gtgtccaaac cgactaccat acctatccga tgggccataa cgcgatcgat ccgggaacgc
    41761 aggaaaacgg atttgaatta cggcgactcc gtcccattat ggattttcgc gtcggtcaat
    41821 tcgtccgcgg gcagatcatg ccggacttct cgagcagctt ccaactgttt aatgccttca
    41881 tcgacgtgga gccttcggac tgggcgagaa tccgcatcgg tctcttcaag ccggtgatga
    41941 acatcgagaa ccaacagggc gaattcgatt tggtgtttgc cgagcgttct atggttcaga
    42001 actttgcgct ccgtcgagat tttggcgtcg aggtgaccgg tcgagtgctg cagcaatcag
    42061 tccgttacga cattggcgtg ttcaacagta ataatggtgc catcggaaac aacagtcggc
    42121 ccaatgtttc cgccgatagt aacaagctgg ggtatgctcg actcatgtat accccgtttc
    42181 tacgcaaggg gccccagatt ttcagaaagc tcgatgtggg cgtcgccggg ttttatggga
    42241 attgtagtca atcggcctgc cagcagccta tgcagaccat ggggtttgac cggacaatct
    42301 tttcgtataa cggtaccgtt accggcaacg gcgctgagac aaaaatcctt cctcaaatgt
    42361 cctggttctg gaaccgcatc ggagtcatgt ccacgttcat tcactcctgg gaaccgaaaa
    42421 ttgaccacgc ctccggcagg tcggacacgc tacagcatca agcctggatg atacaaggcg
    42481 aattcgcgct gacggatgat gagccggcgt tcaaccgtgt ggcacccaaa catccgttcg
    42541 acgcaacctg gaactccttc agccgcggag attggggagc atggacaatt gccgcgcgtt
    42601 attccgaaca agatctggat ccaaacacct ttgggctcaa ttacgggaac gccgccattt
    42661 atgctcgcac cgctaaaggc tacagtgttg ctctgaattg gtatgccagc cgtgagttcc
    42721 gattgcaggg tatctgggag cacacagatt tgaagggagc aaactctacc tacattgcct
    42781 caggcacttc ggacttcgtc attttccgcg tgacgctgat ctactaacct gcggtactga
    42841 atctggtttc gacacgtggg gcgaatcgaa ataacaactc accgacatgt cctttatttt
    42901 cttatgtgct gatggctggc cctgcaagac agtctctcgc aagatggggg gaatcatctc
    42961 caaccaatga cgcctgaatc aataattctg actagcctgg ccggtggcat aggcaacggc
    43021 aacggacgct gcctgcacgc catatctcgc ctctgagagg cgcacttcag caaccaagag
    43081 ggctgtggtg gccgtcgtga cgtccacaat ggaagccaat cccaatcgat agcgttcctt
    43141 ggccagagtc agcgcactgc gggccgactc gacttctttt tcctcgacga ccatctgttg
    43201 acgggccgct tggagcgata aataggcgtc cgtcacctcc agcgcgatgt tattcgacag
    43261 gtttaccttc ttctgctcga ccttgtattt ttgctgtctc gcctctgcga cgcgattttc
    43321 gatcagaaac cccgtaaaaa tcggcacgga caccatcgct cctgcacccc accagagatt
    43381 cgtttgtccg gggtgtgaac cggcatactg gttgagcgga gcgtcactga agtggatgac
    43441 gccgtacatt ccttgagctg cgatcgtggg aagatttaac gcctgagccg aactgagccg
    43501 ttcatccgcc tgcctgatgc gatccgttgt gccgagcaat tccggacggt cttccaatgc
    43561 ctgcgtaatc agggactcca aggtgtatgc cgaggcggcg gcagccggga gatcctccaa
    43621 ggtatagtct tctgatcccc tgactcccat ggcgttattc aatcctgcga aggcagcacg
    43681 cagctcgttt ttcgcctgga gcaactgaac ctcggcattc cggagctcga ccgagatgag
    43741 atcgaagtcc agcttggact tcaattcccg cttgtacaac gtcgcgatct ggtcgcgcag
    43801 gactccacgt tcccggacgg tttcttcggc aatctcgagg agtcggcgtt gccgcaggca
    43861 gttgatgtag gcttgttgga ccttcaggat gacggtcgct ttatgtgtct ggatatcttt
    43921 ttccgccgcc tcctgtccgg atcgctctgc cagaaccttg tgtgccgttt gaccgaaatc
    43981 gtaaatcaac tggtctgctc gaaacccggc gctctcgata taggaagtcg gcttattctg
    44041 tgctcccgcc acgttgaacg cgctgagcgg gcgaaccgtt cctccggttt gaatcgcgta
    44101 cgcattcagt tccggataga atcgagcctc cgcctgcccg gtgacggcct cggcggcaga
    44161 cgccgtttct ttggatcggc gaagcagtgg atgacgatca agggcaatgc gaatcgcagc
    44221 ctcatagccc aggaaccctc ccctcgtctg gggagctgtc ggcgatgaca cagggacgag
    44281 cgatcgaaca ggctcctcag cagaacgctc agccggccag cagagttcac cagacccaag
    44341 cacagcgatc agcatgcacc acatgccggg caaaacccgt ctcgcattcg acaatcgtgc
    44401 cggttcaaaa ccgcccatat tcgactcccg tcgcataggc gagcgcagct tcgctggctc
    44461 gatagtcata ctgcgcctcc gcgagtccga cctcggcgct caacaaatcg gtcgtcgcgg
    44521 tcgtcacatc gagaatggag cctaggctgg ccttataccg ctcgcgtgcg agcgtcagcg
    44581 cttcgcgcgc gtgcgcaacc ctctcctccg cgactttgat ttgttgttca gcggtcaacc
    44641 ggctgaagta ggcttgtgcg acctgcagca cgatatcgtt ggcgatcgat cgagtcgtgg
    44701 cctgagtttc gccctttcga gcgtcggctt cctcaatctg gccttcaatg cggccgccgg
    44761 tatacaaagg gaacgaactg gccaccccaa ctccgtacca accgagcttt gcaccgggat
    44821 tgctcacgtc ttttccactc ggtcgttctt cacgacccca ccaggtatag gccaaggtac
    44881 cgatcgaggt taccgatccg aaccgaaggg ccttcgccgc cttcagagct tcctcggcag
    44941 cctgcacccg gtctttgctt ccgagtaact ccggtcgctg attcaatgct tcctgcacca
    45001 gtgattcagg ggtgggactg ggtatcaccg agagccctgc ttgttccagt acatacgtcc
    45061 ctggaccctg aagacccatg gcggtattca gcgcggtaaa tgccgaagcc agattgttct
    45121 gagctttaat caacaccacc tccgcccgat tgaactccac cgacgcaaaa tccagatcca
    45181 acttcgatcg cagctgccgt cgatagaacg cctccgtctg ctggcggatg agatcacgtt
    45241 cgttcataac ctcgcgcgcg atctccacca accgctgctg cttcagacag ttgagatagg
    45301 cctgctccac atccagaatg gcgagggcct tcgacgtcaa cagggctttc tcagcggacg
    45361 ccgcgaacga tttttgggag aggatccgat gggccgtctg gccgaagtcc gtgataagaa
    45421 aatccgcgcg aatacccgga gtggtcaggt agttcgtagg cttcggcagc gagcccgaga
    45481 cgttgaggtt ggccagcacc ctggtgttgc cggcggaatt ggaataaaga ccggtgatct
    45541 gcggataatt ggccgattcg atctgcttga tcagcgcctg cgactccagc atctcatgct
    45601 cggccacttt gatgagcggg tgtttttcga tagccgtacg aatcgcctgc tcccaactca
    45661 ggcgggagcc tgccgccgac gggagaggca gtgacgtatc ctctgcggcg aacagagtcg
    45721 ccgcgccgga gatgatgcag agcagcagac cagtcaagca cctagaaacg tttgtattct
    45781 tgccctgtcg cataggagac caccgactca ctggttttgt agatgtagcg ggcttccgcc
    45841 aggcgtgatt cggattcaaa caacgctgct gttgcacgga cgacttcgac gatcggattt
    45901 aagccaagac ggtagcgttc ttgcgcaagc atcagcgctt cttttgcaaa ggtcacgcgc
    45961 tcttgttcaa gcccgatttg ttcggcattc gtcgttctcg tgagataggc tcggacaatc
    46021 tgcaacacga tttcattgcc gagattctga agttcctcct ccgtttcccc tttacggtgg
    46081 ctggcttcct tgatctgatt ctgaatccgg aatccggtaa aaatggggaa tttcatcgtg
    46141 gcgccgaatc cccaaaacgg ggctacgcca tcggaaggaa tgcccgcatc atgcacatcg
    46201 ccgtatttgg tgatgccgag ggagccgaca gcggagagcg agccaaagtt cagggccttg
    46261 actgccttga gcaattcttc gctggccacc agacgatccc gattgcccag taattctggc
    46321 cggtcgctga gaccggtggt gaccaatgtt tccactgccg gactgggagt cacttgtatc
    46381 ggaatctgtt ccagttcata ccgctccggc ccttcgattc ccatcgcgtt gttcaaagcg
    46441 gcaaaagatt gcttgagatc gttctgggct ttgatcagtg agagttctgc attcgagact
    46501 tccaccaaca tcaagtcgag atcgactttc gacctgagct ggtgtttgta caacccctgc
    46561 acttgatccc ggaccgcttg ccgttgcttg accgtctcag ccgcgattag gaccagattt
    46621 ttctgcagca ggcagttcag ataggccttt tgaacgttga gaatcacaaa ggctttgtta
    46681 gtcgaaattt ctttctcgct ggctgcctca ttcgcttcat tcgcgaggat gcggtgggcg
    46741 gtatagccga aatcagagat caattgattg agcatgaagc cgccggtcaa catgttctgg
    46801 ttgtgtttcg ccagcgcgcc gccgggatcg aagccgtgac cgccgcgatc gtgaatcgtt
    46861 ttgccatcgg tcgtcacgat gcgcagcgag ccgctggttc cggcgacgga cgcttcaagc
    46921 cagggatacc gttcaccctg tgtttggcgg gtcaacgcct tcgcgatcag ggagctgtac
    46981 cgcgagcgct cgatcagcgg atgggtggca aggccggtcc tgatggcttc atcgagtttc
    47041 aacagagacc ccgccggctt cacgacaggg cgagacacag ccccctcgac cggcgaagta
    47101 aattctcctg tctcagcgag cgcgacctgg agagagttcg cgagaagcgc cagaactaag
    47161 atgccgatga aagtctgtcg catgaggtac cgctctcggc acgcgttttg atcattctca
    47221 tgataaaggg agctacatct tctgtttggc attttggcca gccggcaggt cataatttga
    47281 gacggtcact gcttggccct ccgataaacc acttttgccc acgacaacca cctgttcctc
    47341 gcccgtcagg ccttcaacca cttcaatcga tactccgtcg tcgatgccgg tcctgatggg
    47401 atttcggtgc gcttttccct gttcaacgac gaacacccat ttctcatcgc gtgttttccc
    47461 ggagacaatg gcagcaggcg gaagtgcgag gctgttgggg tgttgctcaa gagtcagttt
    47521 cgccgtcaca aacatccccg gctgaagttc gtgcttttcg ttttgaatgt caatctcgac
    47581 cagtaacgta cgggtggctg gatctaagga ttcggttgtt ctcgtcaccg aaaccttcca
    47641 ctctctgcca gggacctctc tggccgtcaa taccgccgga attccgggtt tggccagcaa
    47701 ggcagtttcc tgaggaacgc tgacatagac gcggagctta ctgagatcca taattgtaaa
    47761 cagaggagcg gcttgtgtag cggaaccggt cgcagattga atcaacgccc ccggatccgc
    47821 aaatcgagcc gtgataacgc cagaaaaggg ggcgtagacc ttcgtgtaat ccatcaagtt
    47881 ccgccggctg tctcgttggt gtttggcgga ctctgccgcg gccttcgcca catccacatc
    47941 ctgcttggca atgatatcgg gattatcctt ccagacgctc aaataccgct cataggtgac
    48001 tttcttgata gcatagtcgg cgtcggcctg tttgtattga tcttcgattt caggagcatc
    48061 gatcacggcc agcagttgac ccttttcgac aatatcccct ttatcaaatc cgatccattt
    48121 cagatagccg gacacttttc cgtagagcgt ggcctgttgc cagggagaga tattagccgg
    48181 gagtgtcagc gtccgcgcaa tatcgcgccg cgtcggcttt agcacttgaa ccgcagggcc
    48241 tccttccgcc tctcctgtcc ccttgctgtc ccggggcggc gaggaaacag attcctctcg
    48301 cccgtttggt ccgaccagcg tcatgatgac gatgagcgcc ccagcaagca cgctccccat
    48361 cacaatatag agacgtttca tcgtccgtgg ctgcatcatg agatcttccc ccatcgaaag
    48421 ctgagacggc gccgccatac ggcctccttc ttcttttgcc agtacccaag gaaccggcct
    48481 gtgttcctgc ttctcggtga tagatactga ttcatccagt gacctgcggt acagggtggc
    48541 ccgtggagcg tccgcgaatc aacgcgtgca atacgggaat gacgagcaga gtcatcacgg
    48601 tggacaccaa caggcccccg atcaccgcac gtgccaatgg catgttcatc tcgctacctt
    48661 cgccgatccc gagcgccatt ggggccagac cgaagacggt cgcgagtgcc gtcatcaaga
    48721 tgggcctgat cctgagccga ccggcctcca ctacggcctg ttcgattggc atgccttcac
    48781 gcataaggtt gttggcaaag tctaccagaa gtacgctgtt cgcgacgaca atgccgatca
    48841 tcatgagcgt cccaatcagg gattctacat tgaccgatgt gtgtgaaagc agcaacatcc
    48901 agatcacccc gatgaatccc agtggtacgg caaagataat gatgaggggg tcaaggtagg
    48961 accggaacaa gccgaccatg acgagataga caaggacaat ggctaacggg agcacaccgg
    49021 cgaagccctt gatagctgat ctcatgctgt ccacttcccc tttgaacatc accgtgacat
    49081 cttttggaag cgaaacgcct gcgacagcct gttcaacctg tgcagccact cgccccaggt
    49141 catagccttt gacattagcc agaacatcga tcgttcgctg gagattatag tggtccaccg
    49201 tctctgggcc agaggtccgc gtgacttggg caatgtccct cagcataatg gagtggcgat
    49261 tgttcccgcc aagaatcggc aactccggac gttcgggaaa cggagtttgc gaccgcgtga
    49321 gagagccgcc tcgacgcaaa gtcccgccta ccgcgacgga gggtcgcaat gaaaccgggg
    49381 aaccgaccag cggaagattg aggaagtctt ggaactcgac cagaagctgc tccgggaact
    49441 gcgtgacgac gaaatacgca ttgttggaac gcggatcaat ccaatatccc ggattcaacg
    49501 tgacgttcga acttagccct gtaatgagat ccgacacaat gtctttctct ttgaggccca
    49561 cataggcagc ctttgcacgg tcgatatcca gatggatttc cgggtagtcc atgccttgac
    49621 ggacttgcac atcggtggtt tggggaacgc gggccacaac attccgaatc tgattcgcgg
    49681 cctcggccaa cgtggagaga tcacgtccgg ttaccttgat gtcgatcggc gccggcacgc
    49741 cgaagttcac gacgtcgctg acaatcccgc cagtctgaaa gctgaatttg agtccgggta
    49801 gccggctgag aaggactccg cgcagcttct caataatcgc atcggtcctt gtcgtatgtt
    49861 cttttttcaa actcacgatg ataaaggcct ggtgtggacc attgactggc gtatagagca
    49921 ccatccatcc ctggggcagg ccgatgttcg acacgatctg ctccaaatca cccgccggta
    49981 cttcttggcg aacgagatcc tcgactcggg caacgatggc ctcggttttc tcaaggcgcg
    50041 ttccctcggg aaccgagacg ttcaggatga acgatcccga atcaactttc ggaaagaact
    50101 ctgtggcaag ctgcgttccg gccagcaagg acacgacgaa gatgaccata accccaagga
    50161 tcactaaggc cttgtgtctc agacaccagc ggagggtctg gacatatcgg ttcacgaggt
    50221 gaccaaacac gcgatcgaac agccgcgatg aagcggcatg atctccgccg ccggcatgac
    50281 cagctggttt cagaagagcg gccatcgcga ccggagcgac cgtcatcgag gagaagtagg
    50341 acaccaccat ggtgtaggca acggccagcg cgagcggcac gaacaaaaag cggatgatgc
    50401 ctgtgaaaaa cataatgggc agatagacaa tcaaaatgct gatcgtaatg accagcatcg
    50461 ggagcgcgac ttcgctcgtt ccgtcgccgg ccgcctgctc aggcgatttt cccatcgcta
    50521 agtgccggtg cacgttttcc aaaaccacga tgttgttgtc taatagagtt ccgatcacca
    50581 acgccaaccc tccaagcgtc atgacgttga ccgactgccc tgtgaaatac agcaaaacaa
    50641 acgccgccat gatcgacagt ggaagggcaa gcgcgatgat cagggtcgat cgcaggctcg
    50701 ccaaaaagat caggaccatg aggcaggcca acccaccgcc cagcagccct tcatgttcca
    50761 gggtctggat ggcttgccga atatacatcg actgatcgga caacaatttc accgacaatt
    50821 ctttcgggag cgcgagtagc ttcgggagga ctgccttgat tccatcgatc acctgaatcg
    50881 tgttcgcccc tgcctgtttc atgatcggga ggtacacggc tcgatggctg tcgatccgga
    50941 ctacgttggt ctgaatcatc gtggaatcga ccgccttccc cacctcgcgc agggtgatcg
    51001 gcaccccgtt gacgactttc agcgggatgt cgttcatctg ctccacgatc ttgatcatgc
    51061 tgttggtata gacgttatag tcgaagaccc cgatcttcac gttgcccgcg gggaggagca
    51121 cgctctgcgc gttgacggaa ttcacgattt cctgggtcga gatgccgcgg gcctgcatcc
    51181 ggtccggatc gagaaagacg gtgatctgcc gcaccgtccc gccaaaggtc gtcggaaagg
    51241 caatccccgg tacattgccc agctggggcc gcacgttaaa gtaggccaga tcccggacct
    51301 ctttctcccc cagtgtctcg ctgctcgtcg tctggacagc gatcggcaag ctcgccgccc
    51361 caaacttgat gatcaccggc ggaaaaatcc cctgcggcag ataccgcagc cccgagtagg
    51421 tcaggctggt aatttcggcg agccccgcat tcacgtcgta cgacggatgg aaataaatct
    51481 tgatgagact cacacccggc agtgaccggg attcgatatg ctcgacatac gaggcctgca
    51541 gaaacagctg ctccaggcgc agcgtgatgg ccccttcgac t
//
LOCUS       DATIFR010000120         1818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2378405, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000120 DATIFR010000000
VERSION     DATIFR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378405"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VLD60_07075"
     CDS             <1..610
                     /locus_tag="VLD60_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44755.1"
                     /translation="GPGTTWWMIDQLKFDQQFLSVLSLIGSTLTLAGMFVFRRFMAER
                     SIAYVVGFLTVIGTVLALPIVSMFYGLHEWTARLTGGIVDARFIALIDTALESPLGQI
                     SMIPMLAWIANSAPANLKATFFAVMASFTNLALSLSQLGTKYLNEIYIVTREIKDAAT
                     GTIQSPADYSELGSLLIVQLLLGLALPFAAIMFAKVTRFKSA"
     gene            complement(656..1339)
                     /locus_tag="VLD60_07080"
     CDS             complement(656..1339)
                     /locus_tag="VLD60_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB44756.1"
                     /translation="MLKTMITRGAGYEVETTDYRMGAVAYHIRTLRDRQQFSDPDGCA
                     ERAGISSASWPMFGIVWPAGLALAKEMTRFPVEGKRILEIGCGIGLPSLVLQQRGADI
                     TACDYHPLTEEFLRHNTDLNGLEPICYFNAPWQIACLELGRFDLIIGSDLLYERDHPS
                     LLAGFLADHANPICQILLADPGRSRCGQISAKLASQGYACTESHLHLAEPPTSVHRGR
                     LMSFVRTHA"
     gene            1638..>1818
                     /gene="ssrA"
                     /locus_tag="VLD60_07085"
     tmRNA           1638..>1818
                     /gene="ssrA"
                     /locus_tag="VLD60_07085"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          376 a          504 c          545 g          393 t
ORIGIN      
        1 cgggcctggg acgacctggt ggatgatcga ccagctcaag ttcgaccaac aatttctctc
       61 ggtcttgtcc ttgatcggga gcacgctcac cctggccggc atgtttgtgt ttcggcgctt
      121 catggccgag cggtcgatcg cctatgtcgt gggatttctc acggtcatcg gcacggtgct
      181 cgcgcttccg atcgtgagca tgttctatgg cttgcatgaa tggacggcga ggttaacggg
      241 cgggatcgtc gatgcgcgtt tcattgcctt gatcgatacg gcgctggagt cgccgctcgg
      301 tcagatctct atgattccca tgttggcctg gattgcgaac agcgcgccag ccaacttgaa
      361 agctaccttc tttgccgtga tggcttcctt caccaatctg gcgttgtcgc tcagtcagct
      421 ggggaccaaa tacttgaatg agatctatat cgttacacgg gaaatcaagg acgccgctac
      481 cggcaccatc caatcgccgg cagactacag cgagcttggc tcactcctca tcgtgcagct
      541 gctcctaggc ttggccctcc cctttgccgc catcatgttt gccaaagtca cccgcttcaa
      601 gagcgcgtag cggttcagcc gaagcgcaga tccgcttttg cgacaagtct ggtgatcacg
      661 cgtgggtccg cacgaaactc atgaggcgcc cccggtggac ggatgtaggc ggctcggcga
      721 gatgaaggtg cgattctgta caggcataac cttgtgatgc cagcttggcg ctgatctggc
      781 cgcaacggct acgccccggg tcagccagaa gaatttggca tatggggttg gcatggtcgg
      841 caagaaaacc ggcgaggagc gaggggtgat cccgctcata caacaaatcg ctgccgataa
      901 tcagatcaaa gcgcccgagt tccaggcagg cgatttgcca tggggcattg aagtagcaga
      961 tcggctcgag cccgttcagg tctgtattat gacgcaagaa ctcttccgtt aagggatggt
     1021 aatcgcaggc ggtgatatcc gcccctcgtt gttggaggac gagactgggg aggccgattc
     1081 cgcagccgat ttcgaggatg cgcttgccct cgaccggaaa ccgggtcatc tccttcgcga
     1141 gcgccaggcc tgcaggccac acaatcccga acatcggcca ggatgcggac gaaataccgg
     1201 cacgttccgc gcatccgtcc gggtctgaga actgctgtcg gtcgcgaagg gttcgaatgt
     1261 gatatgccac cgcccccatt cggtagtcgg tcgtttcgac ctcgtagcct gctcctcgcg
     1321 taatcattgt tttcagcata gacccttctg cggggcaggg ccatgtaaat cacggtccga
     1381 gcatggaaga agacagccat ttcaagtggt cctggtatcc ccttgacctg tgcggtatca
     1441 tgagcatctc ttcaggaagc caagagcaaa tagcacttgg cttatggcgg gatctggaac
     1501 cagcattgga tgatcaaaat gggttccagc aaggccggag cgactgagga cccgaggcgt
     1561 acagtttcac gtacgtcgca gggagcgaag gagcgagaac gccgcgggaa gccattttca
     1621 tcattctgac aagaaagggg ggcgaccggt ttcgacgggg atactgaggt cactgtcgca
     1681 tgtcgagctc ctggggactc gttaaacctc taggaaaatg caactgccaa tcaagaactg
     1741 gcactcgcag cttaaataac tgcgacgttc ctccacctga tgcccgcggg ggtggttgga
     1801 gcgcgatgca gcgggctg
//
LOCUS       DATIFR010000121         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_463960, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000121 DATIFR010000000
VERSION     DATIFR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463960"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VLD60_07090"
     CDS             complement(<1..273)
                     /locus_tag="VLD60_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB44757.1"
                     /translation="MAKAKFERKKPHVNIGTIGHVDHGKTTLTAALTKICADRGMAKF
                     ISYDEVAKASESQGRRDATKIMTIAISHVEYETEKRHYAHVDCPGHA"
     gene            complement(315..>2323)
                     /gene="fusA"
                     /locus_tag="VLD60_07095"
     CDS             complement(315..>2323)
                     /gene="fusA"
                     /locus_tag="VLD60_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSB44758.1"
                     /translation="TTERILYYTGMTHKMGEVHEGAATMDWMEQERERGITITAAATT
                     CFWRDHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFDSVQGVEPQSETVWRQADKYQ
                     VPRIAFMNKMDRIGADFYFSVQTIIDRLGANPIPIQIPIGRESEYRGSIDLITMKAYV
                     YDDETLGAKYKIEEIPADLMDKAKEYREKMLDAVAEFDDQVMEKYLNGQALTEDEIRR
                     VIRAAAISMKVTPVLCGSAFKNKGVQQLLDGVVDFLPSPLDIPPVKCIDPNTDKEVER
                     KASDSEPFAALAFKIMSDPFAGQLTYFRVYSGMLKTGTPVLNVTKGTKDRIGRLLKMH
                     ANKREEIDAVYAGDIVAAVGLKGATTGDTLADEKQPVLLEVMKFPEPVIAMAIEPKTK
                     QDQEKMGFALQKLAQEDPSFRVRTDEETAQTIIAGMGELHLEIIVDRMLREFKVEANV
                     GKPEVAFRETIRRKAEAESKYIKQTGGRGQYGHVVLTVEPSEPGKGLEFVNKVVGGAI
                     PKEYIPAIEKGVKERMENGVIAGYPLRDIRVTVVDGSYHEVDSNEMAFKIAASMGFSD
                     ACKKADAVLLEPIMKVEVLVPQEFMGDVIGNLNGRRGKVQGMKVRAGAQAIEASVPLM
                     EMFGYATDLRSRTQGRATYSMEFDRYEQVPRQIAEAIIAKNRGD"
BASE COUNT          462 a          737 c          568 g          556 t
ORIGIN      
        1 cgcgtggccc gggcagtcga cgtgggcata gtgccgcttc tccgtctcat attccacgtg
       61 gctgatggca atcgtcatga tcttggtggc atcgcggcgc ccttggctct cgctcgcctt
      121 cgccacttcg tcgtagctga taaacttcgc catcccgcga tccgcacaaa tcttcgtcag
      181 cgccgcggtc aacgtcgtct tcccgtggtc cacgtgcccg atcgtcccga tattcacgtg
      241 cggcttcttc cgctcaaatt tcgccttcgc cataccaaac tcctccctgc taccgaaaac
      301 ccgatgatct gttgctagtc gcctcgattt tttgcgatga ttgcttcagc aatttgccgc
      361 ggaacctgtt cgtatcggtc gaactccatg ctgtaggtgg ctcggccctg cgtccgcgac
      421 cgaagatccg tggcatagcc gaacatttcc atcaaaggaa cagacgcttc gatagcctgg
      481 gcccccgccc gcactttcat gccttggact tttccacgac gaccgttcag attgccgatc
      541 acgtccccca tgaactcctg gggaaccaat acctcaacct tcataatcgg ctcgagcaac
      601 acggcatcgg cctttttgca ggcatcggaa aatcccatcg acgcggcgat cttgaacgcc
      661 atttcattgg agtccacttc gtgataggaa ccgtcgacga cggtgacccg tatgtcgcgc
      721 agcggatagc cggcgataac gccgttctcc atccgctcct tcacgccctt ttcaatggcg
      781 ggaatgtatt cctttggaat cgcgccgccg acaactttat tgacgaactc caaccccttg
      841 cctggctcag atggctcgac ggtcaggacc acgtgaccat actgaccacg cccgccggtc
      901 tgcttgatat acttggactc agcctcggcc ttccttctga tcgtttcacg gaaagcgact
      961 tccggcttac cgacattcgc ctcaactttg aattccctca gcatccggtc gacgatgatt
     1021 tcaagatgca attcgcccat gcccgcgata atggtctgcg ccgtttcctc atcggttcgc
     1081 acacggaacg acggatcttc ctgcgcaagc ttctgaaggg caaaacccat cttttcctga
     1141 tcttgcttcg tcttcggctc gatcgccatc gcaatgactg gctccggaaa cttcatgacc
     1201 tcgagcagga ccggctgctt ctcatcggcc aacgtatctc cggtcgttgc ccccttaaga
     1261 ccgacggcgg cgacaatgtc tcccgcgtag actgcatcaa tttcctcacg cttgttcgca
     1321 tgcatcttca ggagacggcc gatgcgatcc ttggtgccct tcgtcacgtt caacaccggc
     1381 gtgcctgtct tcaacatgcc cgaatagacg cgaaagtatg tgagctggcc ggcaaaggga
     1441 tcggacatga ttttgaaggc caacgcggca aagggttcgc tatcggacgc cttgcgctcc
     1501 acttccttat cggtattcgg gtcgatgcat ttcacaggcg gaatatcgag aggggacggc
     1561 agaaaatcga ccacgccgtc cagtaactgc tgtacgcctt tattcttgaa cgccgaaccg
     1621 cacagcactg gcgtcacttt catagaaatt gcggcggcac gaatcacgcg acgaatttca
     1681 tcctcggtca atgcctgccc attgagatac ttttccatca cctggtcgtc gaattccgcc
     1741 accgcgtcga gcatcttttc acgatactct ttggccttat ccatgagatc ggctggaatt
     1801 tcctcaatct tgtactttgc gccaagggtt tcgtcgtcgt acacataggc cttcatcgtg
     1861 atcagatcga tcgagccgcg atattcggac tcccgtccga ttggaatctg gatcggaatg
     1921 ggattagcgc ccaagcgatc gatgatggtc tgcacgctga aataaaagtc ggcgccgatg
     1981 cgatccatct tgttcatgaa ggcaatgcga ggaacctgat acttgtccgc ctgccgccac
     2041 accgtctcgg attgcggctc aaccccttga accgaatcga acaccgccac tgctccatca
     2101 agcacgcgca gcgatcgttc aacctcgatc gtgaaatcaa cgtgaccggg cgtgtcgata
     2161 atgttgatac ggtgatcccg ccaaaagcag gtggtcgccg cagcggtgat cgtaatgccg
     2221 cgctcccgct cctgctccat ccagtccatc gtggccgcgc cctcgtggac ctcgcccatc
     2281 ttgtgcgtca ttcccgtgta atagagaata cgctcagtgg tcg
//
LOCUS       DATIFR010000122         1521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1038665, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000122 DATIFR010000000
VERSION     DATIFR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038665"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1521
                     /locus_tag="VLD60_07100"
     CDS             <1..>1521
                     /locus_tag="VLD60_07100"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSB44759.1"
                     /translation="NAFSKAAGVSVELRDISLAGRILAVFPEYLTPQQKQADALSELG
                     ELAKKPEANIIKLPNISASLPQMQAAIKELQSQGYKLPEYPENPKDDKEKDIKARYDK
                     VKGSAVNPVLREGNSDRRAPLSVKAHTRKHPHKMGAWSPDSKTHVAHMKGGDFRSNEK
                     SMTLSAATEARIEFVGQDGKTTVLKPKVALQAGEVIDATFMSVRALRQFLEEQIEDAK
                     KKGVLFSIHMKATMMKVSDPKIFGHAVTVYYKDVFEKHGETLKKLGVDPDNGIGDLYA
                     KIKALPDDQRKAIEADIQEVYKRRPPMAMVNSDKGITNLHVPSDIIIDASMPPVIRDS
                     GKMWGPDGKLADTKCVIPDASYAPVYHEVVEFCKKHGALDPKTMGSVPNVGLMAQAAE
                     EYGSHDKTFKAPGNGTIRVVDASGKSLLEHKVEEGDIWRMCQVKDAPIRDWVKLAVNR
                     AKATGAPAIFWLDKTRAHDAQLITKVEAYLKDHDTAGLDIRIMTPADATRLSLERI"
BASE COUNT          373 a          418 c          477 g          253 t
ORIGIN      
        1 caacgccttc agcaaggcag ccggtgtgtc ggtggaactg cgggatatct cgctggccgg
       61 acgcattctc gcggtgtttc cggaatacct gacgccgcaa cagaagcagg cggacgcgct
      121 gtccgaattg ggcgagctgg ccaagaagcc ggaggcgaat atcatcaagc tgcccaatat
      181 cagcgcgtcg cttccccaga tgcaggcggc catcaaggaa ttgcagagcc agggctacaa
      241 gcttcccgag tatccggaaa atccgaaaga cgacaaagaa aaagacatca aggcccgcta
      301 cgacaaagtc aaaggcagtg ccgtcaatcc ggtcttgcgc gaaggcaatt cggatcgccg
      361 cgcgccgctt tccgtaaagg cccataccag gaagcacccg cataagatgg gagcctggag
      421 tccggattcc aagacgcacg tcgcccatat gaagggcggg gatttccggt ccaatgagaa
      481 gtcgatgacg ctctctgccg ccaccgaagc ccggatcgag tttgtcggtc aggacggcaa
      541 gacgacggtg cttaaaccga aagtggcgct gcaggcaggg gaagtcatcg acgccacgtt
      601 catgagcgtg cgggccttgc gtcagttcct cgaggagcag atcgaagacg ccaagaaaaa
      661 gggggtgctg ttttcgatcc acatgaaagc taccatgatg aaggtctcgg accccaagat
      721 cttcggccat gccgtgacgg tgtactacaa ggacgtgttc gagaagcacg gcgagacgct
      781 caagaaactg ggcgtggatc cggacaacgg catcggtgac ctctacgcga agatcaaggc
      841 cttgccggac gatcagcgca aggcgattga agccgacatc caagaggtct acaagaggcg
      901 gcctcctatg gcgatggtga attccgacaa gggcatcacc aatctgcacg tgccgagcga
      961 catcatcatc gacgcatcga tgccgccggt cattcgcgac agcggcaaga tgtggggccc
     1021 ggacggcaag ctggcggaca cgaaatgcgt catcccggat gccagctacg caccggtcta
     1081 tcatgaggtg gtcgaattct gtaagaagca tggtgcgctc gatccgaaga cgatgggcag
     1141 cgtgcccaat gtcggtctca tggcgcaggc ggctgaagaa tacggctcgc acgacaagac
     1201 cttcaaagcc ccaggcaacg gcacgatccg cgtggtcgat gcctcgggga agagcttgct
     1261 ggaacacaag gtggaagaag gggacatctg gcgcatgtgc caggtgaagg atgccccgat
     1321 tcgtgactgg gtcaagctgg cggtgaatcg tgcgaaggcc accggtgccc cggcgatttt
     1381 ctggctggac aagaccagag cgcacgatgc gcaattgatc acgaaggtcg aagcctattt
     1441 aaaggatcat gatacagccg ggctcgatat tcggatcatg acgccggccg atgcgacgcg
     1501 cctgtcattg gagcggatca a
//
LOCUS       DATIFR010000123         3669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1039501, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000123 DATIFR010000000
VERSION     DATIFR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039501"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1998
                     /locus_tag="VLD60_07105"
     CDS             319..1998
                     /locus_tag="VLD60_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44760.1"
                     /translation="MSGRNLNNLAAGTGLGAELARLGLLIGLEIRVLTILILYAVAIG
                     LFSLVVPLTVQELTNTFAYAIEPIMIVTFALVMLIGLLTIGFFRVFQTSATETLFQRL
                     YVRIALAMTEHLPRIRQEAFSPGHASRFMEAELLSRAIVVVLVDVINVLVSGMTGMTI
                     LVFYHPNFLIYNTLLLGGFALVAVVAGQGGVLATRTVSEKNYDVMTWIQDIANNRLHF
                     KASQSTPFLIGKTDRLLEAYLRARQVRSNILTWRQYRSIVVWEAVCHSGMIALGGWLL
                     STGQITLGQFVAAEVIVGTLLLNLDTVTRRIYAMTYILTSLGELDRVFALPKHEAFEV
                     GTSIRLPDPALYGVHLTCKDVAFAYPHSPPLFEHVNLEVAPGEKLAVLVRSSLEKSTL
                     ALVLAGLYRPTAGIVRYNDVDLRDVTMDHINGSRGLVLDSQPTLFDGTLEENISLGRA
                     SIRFEDLRWAIRFVELEDEIDRMPHGLDTRVDAGGNRYTKSQILRILVARAIVTRPPL
                     VIFDGTLHNMEPSLRQVLLRRLCSKEEPWVAIFVTNDPALKEYADRSVAVM"
     gene            2096..3490
                     /locus_tag="VLD60_07110"
     CDS             2096..3490
                     /locus_tag="VLD60_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44761.1"
                     /translation="MFRPSPETTLFQIIPLRLIATVLLILALMVSALLLFSFEHEQFV
                     VQGLTEGRDVPTELLGGHWQSRYDLIVMTILLFLLSGIGIAAVITFQHYTSTRRTLEE
                     VKGLARNILESIPTGVVTVNRAGQITAVNPSAVATLKRSTDELLEQSYDKVFAEGDMI
                     RIVLDTALRTNRHVDHHDMPYGTGDQTRTIRVATADLTGDDGRSAGVIVLLKDVTELL
                     DLERRVRVADKLAGLHTLSAGVAHELRNPLSAIDLNLHLLEEELRGGERLSPTTDRYL
                     HVLNAETRRLSAILDTFMKFARPGSVPLHPIDTKQLLSHIISLLQYEAQERRIQLITK
                     ASDTLPPLMGNETEISQVLVNVIVNAFQAMPDGGVCHVLTRGIKTDEKDWVELTVQDS
                     GIGIKKEELSRLFEPFYTTKTGGSGLGLAIAYRIVQDHEGTIQITSQPGLGTTVVVKL
                     PAAPVTQEALVPDR"
     gene            3487..>3669
                     /locus_tag="VLD60_07115"
     CDS             3487..>3669
                     /locus_tag="VLD60_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44762.1"
                     /translation="MTAHAHILVVEDEVNIRSALVTMLEKLGHRVSGAGTAEEALALL
                     EDSPADLVITDLRMPGL"
BASE COUNT          756 a         1120 c         1014 g          779 t
ORIGIN      
        1 gaaatccact ccatcgcact caaatattgt gaccgttgcg cccgtcgttc cccctcactg
       61 ctctatgacg atctcaccct gcaggaactc tgtcaggatt gctgcaatcg gttgaaacgc
      121 cggcgcgaag tggctgttcg tccgacgaca acgggaatgt gagatcgacc tcgtgcggcc
      181 agttccatcg ggaattttcg agggagacgc gcagcacgcc tcggagtccg tccttgtcca
      241 tgaaggggcg acccggtctg ataggttcac gagtccactg catgatcaca attctacggt
      301 tttgactgag ggcgctccgt gtcgggacga aatctgaaca acctggcagc aggaaccggc
      361 ttgggggctg agcttgccag gctcggactc ctgatcggcc tcgaaatcag ggtcctcacc
      421 atcctcatct tatacgccgt cgcgatcggt cttttctccc tggttgttcc gcttaccgtt
      481 caagaactca ccaacacctt cgcctacgca atcgagccca tcatgatcgt caccttcgcg
      541 ctggtcatgc tcatcggcct gctgacgatc ggcttctttc gggtcttcca aaccagtgcg
      601 acggagacgc tctttcaacg actctatgtt cgcatcgcac tcgcgatgac ggaacacctg
      661 ccacgtatca ggcaggaggc gttctcacct ggtcatgctt ctcgtttcat ggaggccgaa
      721 ctgctgtcgc gagccatcgt ggtcgtgctt gttgatgtga tcaacgttct ggtgtcgggg
      781 atgacgggca tgacgattct cgttttctat catcccaatt ttctgattta caacaccctg
      841 cttctcggcg gattcgcgct tgtggcagtg gttgcaggcc agggcggagt cctggccacg
      901 aggacggtgt cggaaaagaa ttacgatgtc atgacctgga ttcaagacat cgcaaacaat
      961 cggctccatt tcaaggcaag tcaaagcacg cccttcctga tcggcaaaac cgatcgtctg
     1021 ctcgaggcgt accttcgagc caggcaggta cgatccaaca ttctgacatg gcgtcagtat
     1081 cggagcatcg tcgtctggga agccgtctgt cacagcggca tgatcgccct ggggggctgg
     1141 ctgttgtcca ccgggcagat cacgctgggg cagtttgtcg cagccgaagt catcgtcggc
     1201 accctgctgc tcaatttgga taccgtcact cgccggatct acgcgatgac ctacattctg
     1261 acatccttgg gtgaactcga ccgtgtcttc gcactgccca aacacgaagc cttcgaagta
     1321 ggcacgagca tccggttgcc ggatcctgca ctctatggcg tccatctcac gtgcaaagac
     1381 gtcgccttcg cctatcccca ctcgccaccc ctcttcgaac acgtcaatct ggaagtggct
     1441 cccggggaaa agctggcggt gttggtacgg tccagcctcg agaagtcgac gctcgcgctg
     1501 gtgctcgccg gcctctaccg gccgaccgcc ggcatcgtcc ggtacaatga tgtggacctt
     1561 cgtgacgtca cgatggacca catcaacgga tcacggggat tggtgctgga ctcccagccc
     1621 acgctcttcg atgggacgct ggaagagaac atctcgctag gacgggcctc tatccgcttc
     1681 gaagatctcc gatgggccat tcgattcgtc gagctcgaag atgaaatcga ccggatgcct
     1741 cacggcctcg acactcgtgt ggatgctggg ggcaaccgat acacgaagag ccagatactt
     1801 cgtattctcg tggcccgcgc aatcgtgacg cgcccgccgc tggtgatctt cgatggaacc
     1861 ttgcacaata tggaaccgag cttacggcaa gttctcctgc gaagactctg ttcaaaagag
     1921 gaaccctggg ttgcgatctt cgtcaccaac gacccggcgt tgaaagagta tgccgaccga
     1981 agcgtcgcag tgatgtaaga ggatcgcctt cttgaacggg cgcctggaac gctccgtgca
     2041 ctgcacattc tcgctgtggt agcatcgtgt actcagcatt cggcggtcaa ctacgatgtt
     2101 tcgaccttcc cctgaaacga cgctctttca aatcatcccc ctgcgtttga tcgccaccgt
     2161 tctgcttatt ctcgccctga tggtgtccgc actcttgctg ttcagtttcg agcatgagca
     2221 gtttgtggtt caaggattga cggagggcag ggacgttccg accgaactgc tcggcgggca
     2281 ctggcaatcc cgctacgacc tcatcgtgat gaccatttta ttattccttc tgagcggcat
     2341 cgggatcgcg gccgtcatca cctttcagca ttacaccagc acacgccgga cccttgaaga
     2401 agtcaaaggc cttgcccgga acattctcga aagcattccg accggcgtcg tcaccgtgaa
     2461 tcgggccggc caaatcaccg cggtcaatcc ctcggcagtg gcgaccttga aacgctccac
     2521 ggatgaattg ctcgagcaga gctatgacaa agtgtttgca gaaggagaca tgatccggat
     2581 cgtcctcgac acagcgctcc gcaccaaccg ccatgtggat catcacgaca tgccctatgg
     2641 gaccggcgac cagacccgca cgatccgcgt cgcgacggca gatctgaccg gagacgacgg
     2701 ccggtcggcc ggcgtgattg tgctgctgaa ggacgtcacg gaactgctgg acctggagcg
     2761 gcgcgtgcga gtggcggaca aactggcggg acttcatacg ctgtcagccg gcgtcgcgca
     2821 cgagctgcgc aatcccttga gtgccatcga tttgaacctc catctcctgg aagaggaact
     2881 gcgcggcggc gaacgactct ccccaacgac agacagatac ctgcacgtct tgaacgcgga
     2941 aacgcgccgg ctttcggcca ttctggacac gtttatgaag ttcgcacgac ccggctcggt
     3001 gccccttcac cctatcgata cgaaacaact gctctcccat atcatctcgc tcttgcagta
     3061 tgaagcacag gagcgccgca tccagctcat caccaaagcg tccgacacgt tgccacctct
     3121 catggggaat gaaaccgaga tcagccaggt cctggtgaac gtcatcgtca acgcgtttca
     3181 agccatgccc gatggcggcg tctgtcacgt cctcacaaga ggaatcaaaa cggacgagaa
     3241 ggattgggtg gagctcaccg tgcaggatag cggcatcggc atcaagaaag aggaattgtc
     3301 ccgtctgttt gaaccgtttt acacgacgaa aaccggcggc agcggattgg gcctcgcaat
     3361 cgcctatcgt atcgtgcagg accacgaggg cacgatccag attacgagcc agccgggcct
     3421 cggtacgacg gtcgtcgtca aacttcctgc cgcccctgtg acccaagagg cgctggtacc
     3481 tgaccgatga ctgcccacgc tcacatcctc gtcgtcgaag acgaggtcaa catccggtcc
     3541 gctctcgtca ccatgctgga aaagctcggg catagggtct cgggagcggg aaccgcggaa
     3601 gaggcgctcg cgttgctcga agactctccc gcggatttgg tgattaccga tctacggatg
     3661 cccggcctg
//
LOCUS       DATIFR010000124        18849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_81241, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000124 DATIFR010000000
VERSION     DATIFR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81241"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VLD60_07120"
     CDS             <1..667
                     /locus_tag="VLD60_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSB44763.1"
                     /translation="MGSTKGPEQQDGKILPVESYGVKLISMAFLVPEEAPLVWRGPMV
                     HQYLQAFFRDVLWGELDYLLLDLPPGTGDVQLSISQMVPLAGAITVTTPQEVALYDVR
                     KGMAMFQKVNVPLLGIIENMSFFVCGHCGERTEIFSYGGGERAAEKLGVPFLGRIPLD
                     PAIRDGGDSGHPIVVANPDSPQAAAFRDIAQKIIAEVGGAGKSGATIDSLLKKIKQPF
                     TSN"
     gene            700..1005
                     /locus_tag="VLD60_07125"
     CDS             700..1005
                     /locus_tag="VLD60_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB44764.1"
                     /translation="MAEFVRVAGTADVKPGHGIVAEVNGKTLAVFNVDGTFHAIDNTC
                     LHRGGPLGEGDVEGNVVTCPWHGWQFDVTTGVCVKNPSAKVEVYQVKVEGSDVKVLL"
     gene            1036..1401
                     /locus_tag="VLD60_07130"
     CDS             1036..1401
                     /locus_tag="VLD60_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44765.1"
                     /translation="MSASTKDQLEVAAAFVRLYVFLTQYLDRCFDEAARKSYPDAELQ
                     AHLTETRRQLMEILAVNPVVKKKLSEECDRILALGAACLKSGAADAKTRETIQAERAV
                     LKNKTMALSDLVAVFRALA"
     gene            1405..1779
                     /locus_tag="VLD60_07135"
     CDS             1405..1779
                     /locus_tag="VLD60_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44766.1"
                     /translation="MSVERFDRHQLAGLDDRERGFSRPVEFERAGENYRAILRYETVR
                     VTTDPHPTQDLALLTLIQTLQSQGYRQLKTQISFRNGVYLGSQELWVEYPDPSQPELE
                     SSGLMTTVMCWVRARMAKRSWT"
     gene            1776..2660
                     /gene="lipA"
                     /locus_tag="VLD60_07140"
     CDS             1776..2660
                     /gene="lipA"
                     /locus_tag="VLD60_07140"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSB44767.1"
                     /translation="MSFISLDQLRSPERPRLPSWFKVVAKTGPDYLEIKQTMDRLKLH
                     TICEEARCPNRWECWNARTATFLILGDICTRRCHYCSVATGRPLAVDHEEPRRVAEAV
                     QALGLRHAVITSVNRDELADGGAAIFAETIRQTRQLNPDCTIEVLIPDFEGSEGALAT
                     VCAEKPEILNHNIETVRRLFPSLRPQGKYQRSIELLGKAKEQGMTTKSGLILGMGETR
                     DEAREVMHDLRAVSCDILTIGQYLQPTREHLPVARFYEPSEFAALKEVGLAMGFVHIE
                     SGPLVRSSYHAEQQALRC"
     gene            complement(2933..3469)
                     /locus_tag="VLD60_07145"
     CDS             complement(2933..3469)
                     /locus_tag="VLD60_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44768.1"
                     /translation="MRRISGRSLKNHFVMARSNLLLMFMLSVLLPLSACEDRRADKAY
                     LRGDYSNSVEELQAMANLEEPRAQYDLALLYDKGLGVPQSDAQALKWYSRSAEHGDQR
                     AQYNLGLMYMNGQGTAPNFILAYYWISLSLAQGNQHAPAAREYLIEKMTPAQIQEANN
                     LVQAHLQNTRPPTIFGNQ"
     gene            3738..4370
                     /locus_tag="VLD60_07150"
     CDS             3738..4370
                     /locus_tag="VLD60_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HSB44769.1"
                     /translation="MSLEFIARLQKELSITGSAVYESVMAVAERVNRKVHIIRLHGQA
                     ASLLTQIESVQGELGRRIATAFPNRPPAPLDTALLTNDLERSLNQATDRIQQLKQTLI
                     QVDAQIRELKLEAIIEDLLTLQRDLSMRAAAIERMTVPQGARAIGKTVSDLSLPPSVR
                     LVTIFRGPFLVPPSDAFVFRPDDVVIVIGLRAELEQLTEWFSPAHSAKSA"
     gene            4426..5829
                     /locus_tag="VLD60_07155"
     CDS             4426..5829
                     /locus_tag="VLD60_07155"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSB44770.1"
                     /translation="MKSLEPPKQAVILATLTFVIGFWSMPPSFAGEPPASASGTSNGL
                     RLLEEIQTVITELAEAAKPSVVSLFPVTTSGKGRELPQERIPNVPGSGSGVIIDTEGH
                     IITNNHVVGDSTEIEVRLSDKTKLIAQVVGKDPDTDLAVLKVTVDRPLPNARFGDSTT
                     VRVGQWVLAVGNPFGLDRTVTLGVVSGIGRENVNLSRYENFIQTDASINPGNSGGPLF
                     NLRGEVIGINTAIINFAQGIGFAIPSNMAKQVLQQLLAQGRVVRGWLGVGIQPLTPEL
                     ARKFGVSESEGVLVNEVFEKDPAAAAGIKPGDIITRIEGAVIDSPNRLSRIVAGLPPG
                     ATTKVEVVRNQQRMVLDVALSERRDQAVVAAIPQSRTEAKLGLDVQDLSAALSEKFKL
                     RESRGVLITKVDPGSLAQSEGLREGDLIKEVNRVDVGSVGEFTSAISKVRRGDTVLLR
                     VLRESRAFYVVLKPSEP"
     gene            complement(5840..7942)
                     /gene="fusA"
                     /locus_tag="VLD60_07160"
     CDS             complement(5840..7942)
                     /gene="fusA"
                     /locus_tag="VLD60_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSB44771.1"
                     /translation="MNESRARSLRNVAIIASRGAGKTSLAEAMLLTTGAIQALGLVAQ
                     GTTVSDFEPEEIQHRSSVSTSLLRCSWNGTTLNLLDTPGALSLLGETLTALQAADAVI
                     LVLNPSSGIRSELMRLWSRVQERRLPCLIYVNQYDKDAASMDDLMAACHRDLDMAPLP
                     MSCLMKKGVQPQGVIDFLSQTAIHSRADTPKWQQQPVDPADDGVVTEARKRIQEAAAE
                     ADDQLLERYLSTGELAQSDLIVGLRSRIQAGSVVPLFVGSALQNTGIVPLLNAVVELL
                     PSPLDRAAQIPIEGVHPESGIAVTRQATASQPFSGIIFKTIIDPFVGRLSYLRMLSGT
                     LHGDTVIFNATRKSKEKGGHLYTLLGKQHATVMSAEAGEIVAMGKLKDAQTGDTLCDE
                     HDPIRHPWPTLPRPVLSFAVEPKSSADVDKVSLGLHKLIEEDPTLGFSRNADTKEMVL
                     SGMGQLHIDLALEKLRRKYGAEVVIHTPKVPYRETIRTTAQAQGKYKKQTGGHGQYGD
                     CWLEIAPSTRGEGFAFENRVVGGVIPRNFIPAVEKGVVEAMHHGTLAGYPVVDIRVAV
                     YDGSYHVVDSSEISFKIAASMAFKKAMESAHPVLLEPMMTVEVDAPADHIGAVIGDLN
                     ARRGRILHVEARGHAEVVTALVPLAEILTYTTTLNSLTGGGGSYVMELTRYDEVPREV
                     ATKIIEEARATQHTVAAH"
     gene            complement(7998..8741)
                     /gene="radC"
                     /locus_tag="VLD60_07165"
     CDS             complement(7998..8741)
                     /gene="radC"
                     /locus_tag="VLD60_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSB44772.1"
                     /translation="MGMKKPGQGINQWPEAERPRERLLAQGATSLTDAQLLAILLRVG
                     RQRSSAVQVGMDLLHRLGGVAGLAHCGVEELCAVPGIGEAKAAQLKAALELGKRALAS
                     PLSKGTRISSSRDLFEHYHPSLRDLRHEIFKVVLLDAKHAIVRESTVSEGSLTLSIVH
                     PREVFTMAVRESAAAVIFLHNHPSGDPTPSHEDRVLTERLVSAGEVLGIRVLDHIVIG
                     DGRYVSFADQGWLREGIRVEAQPRGVVQM"
     gene            8927..9121
                     /locus_tag="VLD60_07170"
     CDS             8927..9121
                     /locus_tag="VLD60_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44773.1"
                     /translation="MECPKCKGLMMLERFSDFFLIFYAWKCINCGAIIDRTISNNRRK
                     SLAARDTQPAPAAQQSVATR"
     gene            9407..9979
                     /gene="rsmD"
                     /locus_tag="VLD60_07175"
     CDS             9407..9979
                     /gene="rsmD"
                     /locus_tag="VLD60_07175"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSB44774.1"
                     /translation="MRVIAGSHRGRRLCGPQGTTLRPTSDMVREALFSILGARVPGSR
                     FLDLYAGTGAVGIEAFSRGADVVTFVESDLKAAQLMRKNLTTCHLLDRADIRLATAKS
                     FLQRSDWWKGPYDLLFADPPYGDSDQEELIRAAWQPGLLRDRALVVLEQDSRSELPAL
                     IDHAKLIRRYVYGDTALFVYGSSPTEVVTS"
     gene            9976..10473
                     /gene="coaD"
                     /locus_tag="VLD60_07180"
     CDS             9976..10473
                     /gene="coaD"
                     /locus_tag="VLD60_07180"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSB44775.1"
                     /translation="MKIGIYPGTFDPITHGHTDIITRSLRVFDKVIVAVAPNPAKHPL
                     FNLTERLEMVELVMKDQGQGGQVEVVSFDGLLVDYVQRSGSHAIIRGLRAISDFEHEF
                     QMALINRKLAKNVETVFLMPSEEYSYLSSTIIKDIANHGGSLTAFLHPEVARRLQERI
                     RSLKR"
     gene            10470..11663
                     /locus_tag="VLD60_07185"
     CDS             10470..11663
                     /locus_tag="VLD60_07185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSB44776.1"
                     /translation="MKLATRVGRIAPSPTLAMAATAKAMAAQGIDVIDFSTGEPDFDT
                     PEPVKAAAEAAIRSGFTKYTPSSGIDELRGAIVEKLKTEQGLQYEKSQVLVSCGAKHS
                     LYNLAEALLEAGDEIIIPTPYWVSYSDQALLNDATAVLLPTKEEDGYAIHPEELEQRI
                     TARTKAIIINSPCNPTGATYNRKMLEGVATIAVRRDLLVISDEIYEKVLYDGATHVSI
                     ATLGPEIAARTVVINGVSKAYAMTGWRIGYAAGPKELITAMGNIQSQSTSNPCSISQK
                     AAVAALRFGEPFTKKMVAEFDRRRQVIVEGLNKIPGVSCRMPGGAFYAFPNIKGMLGR
                     RGPHGPINSPTDLATYLLKDAHIAVVPGEPFGSQQHIRLSYATSMEMIKRGIDRLGVA
                     LQKLT"
     gene            11717..12088
                     /locus_tag="VLD60_07190"
     CDS             11717..12088
                     /locus_tag="VLD60_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSB44777.1"
                     /translation="MPIYEYLCEGCAYKFEVKQSMKDDPIATCERCGKPVQRLISSPA
                     IMFKGSGWYITDYSDKMKPPTDGEKPASTSGEKKETTSTPASPAVPAPAGASTPTQAS
                     TTSSAPAPSPSSSSTTSSSEK"
     gene            complement(12105..12521)
                     /locus_tag="VLD60_07195"
     CDS             complement(12105..12521)
                     /locus_tag="VLD60_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44778.1"
                     /translation="MTMGRRVHQWFGGVLILGLAVGIAGCDYWPPALQTQIEQLRSEL
                     QTVTMEKTQLQNQLASVTKVKDDLQIQVDELSRANRDKSSLISSLQNTVTSLQERLAK
                     SSKPATVKPSSTKPAAKTPQKAPVKKKTASKSVVVR"
     gene            complement(12564..13208)
                     /locus_tag="VLD60_07200"
     CDS             complement(12564..13208)
                     /locus_tag="VLD60_07200"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSB44779.1"
                     /translation="MAEIIPYSPDALPHLIRRVSERLYQGGIVAIPTETFYGLAVDPF
                     DEAAVERLRRLKGREDGKPILVLIGSMDQLPLLAREVPSIAKILIEAFWPGPLTILFP
                     AVPALPVNLTAGTGLVGIRLSSSKPLMALLAHVGPLTGTSANLAAQPPARTAMMVEQA
                     LGQEVDLIVDAGTTAGGLPSTVIDVQQPLRVVRDGAVTRQMIQNVLQTHGISLV"
     gene            complement(13242..14510)
                     /gene="purD"
                     /locus_tag="VLD60_07205"
     CDS             complement(13242..14510)
                     /gene="purD"
                     /locus_tag="VLD60_07205"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSB44780.1"
                     /translation="MKILVVGSGGREHTMVWKLAQSPRKPQLFCAPGNAGIESLAACV
                     PIKADDIAGLREFVIREQIDLTLVGPEAPLALGIADEFRKAKLRIFGPTRNAARLEAS
                     KIFSKDIMARAKIPTAHAQHFHKLADALAYVEQHELPVVIKADGLAQGKGVIIATTRD
                     AARQAIIDSMDKAVFGQAGKQVLIEQFLDGEELTIMAFTDGRTVAPMLPAQDHKRIGD
                     GDTGLNTGGMGAYCPAPLGTLTLRQQVTREILQPIVETMSRSGSPFQGVLYAGLMVVK
                     GVPYVLEFNARMGDPETQVVLPLLKTDLIDVAEAVVEHRLDQLTVEWHDDHAVCVVMT
                     SGGYPGTYQSGFPIRGLNSSEHKATVVFHAGTAKKNGDVVTAGGRVLGITGLGKTLEE
                     AQARAYRAVNTISFEGCHFRKDIAHRALNR"
     gene            complement(14660..16213)
                     /gene="purH"
                     /locus_tag="VLD60_07210"
     CDS             complement(14660..16213)
                     /gene="purH"
                     /locus_tag="VLD60_07210"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSB44781.1"
                     /translation="MASIKRALVSVSDKTGVIEMAKGLEALGAEILSTGGTAKALRDA
                     GVKVTDVAAYTGSPEILEGRVKTLHPKIHGGLLGRRSVPAHVEQMRQHNIGPIDVVVV
                     NLYPFEATIAKPNCSFDEAIENIDIGGPSMLRSAAKNHEDVLVVVDPADYSRVLDAAK
                     SGNVPHALRRELAMKVFQHTARYDSLIAGYLERQMQGGEVKFPKILSLQYELGETLRY
                     GENPHQQGAFYRELHSNEPSVSRGKILHGKAMSYNNFLDANSALELAKEYDETAVAII
                     KHNNPCGVALGATPVEAYVKARETDPISAFGGVIAFNRPVDLAAAKEITSTFVEVVIA
                     PGFTDDALAELKRKKDLRLLDVGPLTKVKQEGYDLKKLVGGLIVQDRDLGVLTDLRAL
                     PVPTVRKPTDEEYAACAFAWKVCKHVKSNAIIYAKPGQTVGIGAGQMSRVDSVKLAAM
                     KAQMPVKGCVMASDAFFPFRDGLDAAAQAGITAVIQPGGSIRDAEVVKAADEHGLAMI
                     LTGMRHFRH"
     gene            16568..17428
                     /locus_tag="VLD60_07215"
     CDS             16568..17428
                     /locus_tag="VLD60_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB44782.1"
                     /translation="MGRRIILGAGAFALTLLALVCIPRHLPSSNASLPLVPANFHARL
                     EQHELTLRGSLPDRVTHDRILQQAHSVYDRAQVRIVDQLTVDQRIAPAAWLATVPSIL
                     PVLGDMNGRGSMIIDGHYLVLSGRVPTEQVKGAILRSVAPATATGLELEDHILAATQP
                     ASRFSLQAQLNEVLSRNQIEFDSNQSTLTSRGRSALDRLIPLLRQAPRASIEIGGHTD
                     GFGAPDYNRELSRRRAETVRQYFIQHGLTNRFTVVGYGASKPRSTEMTQNGLRRNRRI
                     ELRVQEQGEL"
     gene            17425..18600
                     /locus_tag="VLD60_07220"
     CDS             17425..18600
                     /locus_tag="VLD60_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44783.1"
                     /translation="MMVLIGQMVGCLLVAAAIGGAVGWLLRHLSAAPIEQRLIDSETD
                     LRIKGQALDTALYELRVNKSTLATLEAKLSSMESLGRSTQQELTSRQDRIQTLQKELA
                     NSQQRLSAMESDHKASLQRLTEQETAMAAFANEARRANAARTAAQQDLLLKEQSASEL
                     QQRLTELEDQQVEIDRLRAQVAELEPVQGRLHWMEVQLSEKETQHRANLHRLEEQLAF
                     RDRRIAELAELEQALREKDLRIAELEQRLAEADVLHTELAGQAKMMGEKEEEISRLRK
                     RLVEVRAALRVRADGGHVVTGRANSTNQLSLQIEHSKQTQKPQKDDLKKIHGIGPVIE
                     RTLNKLGTYTYVQIAKWTPSDIARVAQKLETIPDRIKRDNWIASARKLYKEKYGERI"
     gene            18661..>18849
                     /locus_tag="VLD60_07225"
     CDS             18661..>18849
                     /locus_tag="VLD60_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSB44784.1"
                     /translation="MASSFVHLHLHTQFSLLDGANQIEPLVQQIKAFGQPAVAMTDHG
                     NMFGAIEFYRKAKEAGVKP"
BASE COUNT         4134 a         5569 c         5160 g         3986 t
ORIGIN      
        1 gatggggagc actaagggac ccgagcagca ggacggcaag atcctgccgg ttgaaagcta
       61 tggggttaaa ctcatttcca tggcgttcct ggttccggag gaagcgccgc tggtctggcg
      121 cgggccaatg gtgcatcaat acttgcaagc attcttccgc gacgtcttgt ggggcgaact
      181 ggattatttg ctgctcgacc tgcctcctgg gaccggcgac gtccaactct ccatctcgca
      241 aatggtcccg ctggccggcg ccattaccgt gacgaccccg caggaagtcg cactctacga
      301 tgtccgcaag ggcatggcga tgtttcaaaa agtcaatgtg ccgcttctcg gcatcattga
      361 gaacatgagc ttcttcgtgt gcggccattg cggcgaacga acagaaattt tctcctacgg
      421 gggaggagag cgggcagcgg agaaacttgg cgtgccgttt cttgggcgta tcccgctcga
      481 tcccgcgatt cgcgatggcg gcgacagcgg ccatcccatc gtggtggcca atccggattc
      541 tccgcaagcg gcggcgttca gggacatcgc gcagaagatc attgcggaag tgggtggcgc
      601 ggggaagagc ggcgccacga tcgacagcct gctcaagaag atcaagcaac ctttcaccag
      661 taactaatcg aaggttcatc caagcagagg agagcggtta tggcagagtt tgtacgtgta
      721 gcaggtacgg cagatgtgaa acccggtcac ggcatcgtgg cggaagtgaa cggcaaaacc
      781 ctggcggtct tcaacgtcga tggaaccttt cacgccatcg acaatacctg cctccatcgc
      841 ggcggcccgc ttggtgaagg ggacgtcgaa ggaaatgtcg tgacctgccc gtggcatggc
      901 tggcagtttg acgtgacgac aggcgtttgt gtgaagaatc cctctgcgaa agtggaggtg
      961 tatcaagtga aggtcgaagg ctctgacgtc aaggttctcc tctaactccg cgaccaagga
     1021 aaggaggggc gccgcatgtc cgcctctaca aaagaccagc tcgaagttgc cgcggccttc
     1081 gttcgtctct atgtcttcct gacccaatat ctcgaccgct gtttcgacga agcggctcgc
     1141 aagagttatc ctgatgccga gcttcaggcg catctgacgg agacacgtcg ccagctcatg
     1201 gaaattctgg ctgtcaatcc cgtggtcaaa aagaagctct cggaggagtg cgatcgcatt
     1261 ctagcgctgg gtgccgcctg cttgaaatcc ggcgcggcgg acgccaagac ccgcgagacg
     1321 atccaagccg aacgagccgt gctcaagaac aagaccatgg cattgagcga cttggttgcc
     1381 gtctttcgtg cgctcgcatg acaaatgagt gtagagcgat tcgataggca tcagctggcc
     1441 ggcctcgatg atcgggaacg gggattcagc cggccggtcg agtttgagcg ggcaggggag
     1501 aactaccggg caatcttgcg atatgaaacg gttcgtgtga ccaccgaccc acacccgaca
     1561 caggatctcg cattgctgac cttgatccag actctgcaat cgcaaggcta ccgccaactg
     1621 aagacacaaa taagctttcg caatggcgtc tatctcggct ctcaagaatt gtgggttgaa
     1681 taccctgacc cgtcgcagcc tgaactggaa tcgtcaggac tcatgacaac ggtgatgtgc
     1741 tgggttcgtg ctcgaatggc caagagatca tggacatgag ctttatttct ctcgatcaac
     1801 tccggtcccc cgaacgtcca cgacttccat cgtggttcaa agtggttgcg aaaaccggcc
     1861 cagactatct cgagattaag cagaccatgg accggctcaa gctccacacc atttgtgaag
     1921 aagcccgctg cccgaaccgg tgggaatgtt ggaacgcccg caccgccacc tttctgatcc
     1981 tgggtgatat ctgcaccaga cggtgccatt actgttcggt ggctaccggg cgcccgttag
     2041 cagtcgatca cgaagaaccc aggcgcgtgg ccgaagcggt ccaggcgttg ggccttcgtc
     2101 atgccgtcat cacgtcggtg aatcgagacg agttggcaga cggcggcgcg gcaatcttcg
     2161 ccgaaacgat ccggcagacg agacagctca acccagattg cacgattgaa gtcttgatcc
     2221 cggatttcga ggggagcgag ggagcattag cgacggtctg tgccgagaag ccggagatcc
     2281 tgaatcacaa cattgaaacg gttcgacggc tgttcccatc tctcagaccg caaggcaaat
     2341 accagcgttc catcgaacta ctcggaaaag cgaaggagca ggggatgacc accaaatcag
     2401 gcttgatcct tggaatggga gaaacgagag atgaagcgcg tgaggtcatg cacgacctgc
     2461 gcgcggtttc ctgcgacatc cttaccatcg gccaatatct tcagccgacg agagaacatc
     2521 tgccggttgc ccgattctac gagccgtccg agttcgcggc attgaaagaa gtaggcctcg
     2581 ccatggggtt cgtgcacatc gaatcgggac cgctcgtgcg cagttcctat catgcagaac
     2641 aacaagcctt gcgatgttga tcgattggaa gataatttct tgcctgatcc tgacagggct
     2701 aagacaatca gcgcccgcgc ttcttcctga atccgcttct tgatactctt tccccctaga
     2761 aaattatgcg tcgagtccgt ctcctctccg ataacggacc tgctaacgct gtacgcagct
     2821 cgcggatatc caggttcatg tctggtgctg ctgcatcaat caatgacgta atgatgatca
     2881 aggaggcaag gaggagggac tgcctttagc ggttgcctac tggttaccga agttactggt
     2941 taccgaagat ggttggaggg cgggtatttt gcaagtgcgc ttggacgagg ttattggctt
     3001 cctggatttg ggccggtgtc attttttcga tcaggtattc ccgggcagcc ggagcgtgct
     3061 ggttgccttg tgccagggaa aggctgatcc agtagtaggc gagtatgaag ttgggggctg
     3121 tcccttgtcc gttcatatac atgagcccaa gattatactg ggctcgttga tcgccgtgtt
     3181 cggctgaacg gctgtaccat ttcaatgcct gagcgtcgct ctggggcacg ccgagcccct
     3241 tatcgtacag gagagcaagg tcatactgcg ccctcggctc ttcgaggttt gccatggcct
     3301 gcaactcttc gacgctattg ctgtaatctc cgcggaggta tgccttgtcg gcgcgccgat
     3361 cctcacaggc agagagtgga agcagcacgc ttagcatgaa catgaggagg agattggatc
     3421 gcgccatgac gaagtggttc ttgagcgatc gtccagagat ccgacgcatt tcaggtgaat
     3481 ccgcgaattt cacgatctag cctaatgagt gagctaacca ttgtcaaata tcgagaatta
     3541 ggccgtttcc tttccctctg ttgaccctgc agaatgtaac gcagaattct gatcgacctg
     3601 atagaccgac aatggtatcg tggacaggta tgcctgtggg aaggacgata gtgaggccat
     3661 tttcaagtcg atcggattgc tagtgactag ttgacataat aacacttatc agacgttgca
     3721 ttcgaagagc actcgatatg tcccttgaat ttattgcccg cctgcaaaaa gaactctcga
     3781 tcactggcag cgccgtctac gaatcggtca tggccgttgc tgaacgagtc aaccgcaagg
     3841 tccatatcat tcggctccac ggacaggctg caagcctcct gacccaaatc gaatcagtcc
     3901 agggagagct cggacgtcgt atagccaccg ccttcccaaa tcgaccgcct gcgccactcg
     3961 atacagccct gttgacgaac gacctcgagc gatcgctgaa tcaggctacg gatcgcatcc
     4021 aacaactcaa gcagaccctc atccaagtcg atgcccaaat tcgcgagttg aagctggagg
     4081 caatcatcga ggacctgttg accctgcaac gagacctgag catgcgggcc gccgctatcg
     4141 aacgtatgac agtgccacag ggggcccggg cgatcggcaa gaccgtctcg gatctctcct
     4201 tacccccctc ggttcggttg gtaaccattt tccgcggccc cttcctggtg ccgccttcgg
     4261 atgcctttgt attcagacca gacgatgtcg tgatcgtgat cggcctccga gctgaacttg
     4321 agcagctcac cgagtggttt agcccagcac attcagccaa atcggcatag tggttgaact
     4381 tctctcccca taggcttttc tccctgttca atgtagaatg attttatgaa gtctctggaa
     4441 ccacccaagc aagcggtcat cctcgcgaca ctcacattcg tcatcggttt ctggtcgatg
     4501 ccgccttcct ttgcgggtga gccgccggct tcggcctccg gcacctcaaa tggcttgcgc
     4561 ctgctcgaag aaattcaaac ggtcatcacg gaattggcgg aagccgcgaa accttccgtc
     4621 gtcagccttt tccccgtgac gacctccgga aagggacggg aattgcctca ggagcgtatt
     4681 ccgaacgttc ccggttcagg ttctggcgtc atcattgata cggaaggaca catcatcacg
     4741 aacaaccacg tcgtcggcga ttcgaccgaa attgaagtgc gcctctccga caagaccaag
     4801 ctgattgcgc aggtcgtcgg caaagatccc gacacggatt tggcggtact caaagtcacg
     4861 gtcgaccgtc ctcttcccaa tgcgcggttc ggggactcga cgaccgtccg agtcggccag
     4921 tgggtcttgg ccgtcggcaa ccccttcgga ttggatcgca cggtgacact cggcgttgtc
     4981 agcggcatcg gtcgagagaa tgtcaatctc tctcgttacg aaaatttcat tcagaccgat
     5041 gcctccatca atcccggcaa ttcaggcgga ccgctgttca acttgcgtgg cgaggtgatc
     5101 ggcatcaaca ccgcaatcat caatttcgca cagggtatcg gctttgccat cccctccaac
     5161 atggccaaac aagttctcca gcaattattg gcgcagggtc gcgtcgtccg aggatggctc
     5221 ggggtgggaa ttcaaccgtt gacacctgaa ctcgcgagaa aattcggcgt cagcgagagc
     5281 gagggtgtct tggtcaacga agtgttcgag aaagatccgg ccgccgcagc cggcatcaaa
     5341 ccaggcgata tcattacccg cattgaaggc gctgtcatcg actcacccaa tcgactctcg
     5401 cgcattgtcg ccggcttgcc gccaggcgcc acgacgaaag tcgaagttgt gcgaaatcaa
     5461 cagcggatgg tcttggacgt cgccttgagt gaacgccgtg atcaagccgt cgtggccgcg
     5521 atccctcaat cccgtactga agcgaagctg ggtctcgatg tgcaggactt gtccgcggcg
     5581 ctctccgaaa aattcaagct ccgtgaatct cgcggcgtgc tgatcaccaa ggtcgacccg
     5641 ggcagcctcg cgcaatccga gggattgcgc gagggcgatc tgatcaagga agtgaaccgc
     5701 gtggacgtcg ggtcggtcgg cgagtttacc agcgccatct cgaaggttcg ccggggcgac
     5761 accgtgctgc tccgcgttct ccgagaaagc cgcgccttct acgtggtgtt gaagcccagc
     5821 gagccttaag acccttccct taatgcgctg cgaccgtatg ctgagtcgca cgggcttctt
     5881 caataatctt tgtcgccacc tcccgcggca cttcgtcgta gcgcgtcaat tccatcacat
     5941 aactccctcc cccgcccgtg agagaattga gcgtggtcgt ataggtcagg atctccgcca
     6001 gtggcacgag ggcggtcacg acttccgcat gccccctggc ctcgacatga agaatccgcc
     6061 ctcgtcgcgc atttaagtcc ccgatcaccg cgccgatatg atcggccggc gcgtccacct
     6121 ccacggtcat catcggctcc agcaacactg ggtgagctga ctccatcgct ttcttgaacg
     6181 ccatcgaggc ggcgatcttg aacgagattt cggaagaatc gacgacatga tatgacccgt
     6241 catataccgc tacgcgaatg tcgaccacgg ggtagccggc cagcgtgcca tgatgcatgg
     6301 cttccacgac gcctttttcg actgcaggaa tgaaattgcg cggaatcacc ccgccgacta
     6361 cgcggttttc gaaggcaaac ccctccccgc gagttgacgg cgcaatctca agccaacagt
     6421 cgccatactg cccgtgaccg ccggtttgct tcttgtactt accctgtgct tgggcggtcg
     6481 tccggatggt ttcgcggtat ggaactttgg gggtgtggat cacgacctcc gcgccgtact
     6541 tgcgacgcaa tttctccaac gcgagatcaa tatgcaactg gcccatccca ctcagtacca
     6601 tctccttggt atccgcattg cgggagaacc ctaacgtcgg atcttcctcg attaatttgt
     6661 gaagtccaag actcaccttg tcgacatcgg cgctggattt cggttccacg gcaaaggaca
     6721 acaccgggcg aggcaacgtc ggccagggat gacggatcgg atcatgctcg tcacagagcg
     6781 tatccccggt ttgtgcatct ttgagtttcc ccatcgcgac aatctcgcct gcttccgccg
     6841 acatcactgt cgcgtgctgt tttcccagaa gggtatacaa atgtccgcct ttctccttgc
     6901 tctttcttgt cgcattgaag atcacggtat cgccatggag ggtgccggac agcatgcgaa
     6961 gataggagag ccgtccgaca aatggatcga tgatggtttt gaagatgata ccggaaaatg
     7021 gctggctggc agtcgcttgc ctggttaccg caattccgct ttccggatgc acgccctcga
     7081 tcgggatttg cgccgcccgg tcaagtggtg aaggcaagag ctcgaccact gcgttcaaga
     7141 gcgggacaat tcctgtattt tgcagcgcag agccgacaaa cagtggcacg acagagccgg
     7201 cttggattcg acttcggagg ccgacgatca gatcggattg tgccaactcc cctgtcgaga
     7261 gataccgctc caacaattga tcgtccgcct ccgctgctgc ctcctgaatc cgcttccgcg
     7321 cctccgttac gacaccgtca tcagcagggt caactggttg ctgttgccac ttcggcgtgt
     7381 ctgcgcgcga atggatcgcg gtctggctga gaaaatcaat gaccccttgc ggttgcacgc
     7441 ccttcttcat cagacaggac atcggcaacg gcgccatatc gagatcgcga tggcaagccg
     7501 ccatgagatc gtccatcgag gccgcgtcct tatcgtactg gttgacatag atcagacacg
     7561 gaagccggcg ctcctggacg cgagaccaga gtctcatcaa ttcgcttctg atcccgctgg
     7621 agggattgag cacaagaata acggcgtcgg cggcttgcaa agccgtgagc gtttctccca
     7681 gcagactaag cgctccaggc gtgtcgagaa gattgagggt ggtgccgttc cacgaacagc
     7741 gaaggagact ggtactgacc gagctacggt gctgaatttc ctccggttcg aaatccgaca
     7801 cggtcgtgcc ttgcgccaca agtccgagag cttgaatggc tccggtcgtc aggagcatcg
     7861 cttcggccaa cgaggtcttg cccgcacctc ggcttgcgat gatggccaca tttctgagag
     7921 acctcgcacg cgactcgttc atagagccct cctcggtcat gcggagagtg taggcgccct
     7981 ccggcagtgg ttaggtctta catctgcacg actcctctcg gttgtgcttc aactcgaatc
     8041 ccctctctca gccaaccttg atcggcaaaa ctgacatagc gaccatcacc gatcacgatg
     8101 tgatccagta ctctgatccc cagcacctcc cctgcggaga ccaatctctc cgtcaggacc
     8161 cggtcttcgt gactgggcgt cggatcaccg ctgggatggt tatgcagaaa aatcaccgcc
     8221 gccgccgatt ccctcaccgc catcgtaaaa acttcgcgtg gatgcacgat actcagcgtc
     8281 aggcttccct ccgataccgt tgattcacgc acaatcgcat gcttggcatc caggagtacg
     8341 actttgaaga tttcgtggcg gaggtcgcgc agagacggat gataatgctc gaaaagatcg
     8401 cggcttgagg aaatccttgt ccctttggag agcggcgacg ccagggctcg tttgcctaac
     8461 tccagggcag ccttgagttg ggcagccttc gcctccccta tcccaggaac cgcgcagagc
     8521 tcttcgacgc cacagtgcgc caaaccggca acaccaccaa gcctgtggag aagatccatg
     8581 ccgacttgta cggcagatga tcgttgacga ccgacccgaa gcagaattgc aagcagttgt
     8641 gcatcggtga gtgaagtggc cccctgagca aggagccgtt cgcgaggacg ttcagcttcc
     8701 ggccactggt tgatgccttg tccgggtttc ttcatcccca tgcgaactct ccacgaacaa
     8761 aaatatgaca cgttgaacgt ttttgtacta gctacacacc ctctgttcgc cccttggtgt
     8821 caaatacgta ccttctaaag tgttgaaatt acgtatcgcg acaatttggt gcagctcttg
     8881 ctttggtacg cacccagctg tgaacgttct aacctgggag gaatcgatgg aatgccccaa
     8941 gtgtaaaggt ctcatgatgt tggaacggtt ctccgacttc ttcctgatct tctatgcctg
     9001 gaagtgcatt aactgcggcg cgatcattga tcgcacaatc tccaataatc gacggaagag
     9061 cttagcagct cgcgacacgc aaccagcccc agcggcacag caatcagtgg ccacgcgtta
     9121 acgaaccgct cccgatcctg ttgcgagcac gtcgtgacgt cgtggccaac cagatgacga
     9181 ctggctaggc gctttctcca gctcatccat cccccgctgc aggattatag tcagcctgct
     9241 gcactccgtc aaaataggac caattcagat cagtatccca cagcaatacc ctcgtgattt
     9301 tcttccggat atctatcagc gcttcgtctg agtttgctga ccttgacccc tgcaaaaacg
     9361 ctatgttaca gtctgttact cctgaacggc ctctcacctg aaccttatgc gagtcatagc
     9421 cggttcccat cgaggtcgac gcctctgtgg accacagggg acaacgcttc gtcccacgtc
     9481 ggatatggtg cgcgaggcgc tgttttctat tcttggagct cgcgtaccag ggagccggtt
     9541 tcttgatctt tatgctggaa caggagccgt ggggattgaa gccttcagcc gtggagcaga
     9601 cgtcgtgacc ttcgtcgagt cagatctcaa ggccgcgcaa ttgatgcgaa aaaatctcac
     9661 aacctgccat cttcttgatc gagcggatat ccgactcgcg accgcaaaaa gcttcctaca
     9721 acgatccgat tggtggaaag ggccgtatga tctcctcttt gccgatccac cctatgggga
     9781 ctccgatcaa gaagaactga tccgcgccgc ctggcaacct ggtctcttac gcgaccgcgc
     9841 tctcgtcgtt ctcgagcaag actcacgatc ggaactaccg gccttgatag accatgcgaa
     9901 actgattcgc cgctatgtct acggcgacac ggcgctgttc gtatacggat catcgcccac
     9961 ggaagttgtg acctcatgaa aatcggtatc tatcccggca cctttgatcc catcacgcac
    10021 ggccacaccg acatcattac acggagtctt cgcgtgtttg acaaggtgat tgtggccgtc
    10081 gcgccaaatc cggccaagca tcccctgttc aatctcaccg aacggctgga aatggtggag
    10141 ctggtgatga aagatcaggg gcagggcgga caggtggaag tggtgagctt cgacggcctc
    10201 ctcgtcgatt atgttcaacg atccggatcc catgccatca tccgcggtct tcgagctatt
    10261 tccgatttcg agcatgagtt tcagatggcg ctcatcaatc gcaaactggc caagaacgta
    10321 gaaaccgtct tcttaatgcc gagtgaagaa tattcatatc tctcctcgac cattattaaa
    10381 gacattgcaa atcacggcgg gtcattgacg gcctttctcc atcccgaagt ggcgcgccgc
    10441 cttcaagaac ggattcggag cctcaaaaga tgaagctggc aacccgcgta ggccgtattg
    10501 ctccctcgcc gacgttggcg atggccgcca ccgccaaggc tatggcggca caaggcatcg
    10561 atgtcatcga cttttcgacc ggcgaaccgg atttcgatac gcccgaaccg gtcaaggcag
    10621 cggcggaagc ggctattcga tcgggtttca caaaatatac cccctcttcc ggcatcgatg
    10681 aactccgggg cgccattgtt gagaaactca agaccgaaca gggactccag tacgagaagt
    10741 cgcaggtgct cgtgtcctgc ggcgcaaagc attcgctgta caaccttgcc gaagcgctgc
    10801 tggaggcagg agatgaaatc atcatcccga ctccttactg ggtatcctat tccgatcaag
    10861 cgttgttgaa cgatgcgact gcagtgctgc ttccgacaaa ggaagaagac gggtacgcga
    10921 tccatccgga ggaattggaa cagcgcatta ccgctcgaac gaaagccatt atcatcaata
    10981 gcccctgcaa tccgaccggc gcgacctata atcggaaaat gctggaaggc gtcgccacga
    11041 tcgccgttcg acgcgacctg cttgtgatct cggacgagat ttatgaaaaa gtgctgtatg
    11101 acggagctac ccacgtcagc atcgcgacgc tgggaccgga aatcgcggcg cggactgtgg
    11161 tcatcaacgg cgtctcaaaa gcctatgcca tgaccgggtg gcgaatcggc tatgcggcag
    11221 gaccgaaaga actcattacg gcgatgggca atattcaaag ccagagtacc tcgaacccct
    11281 gttcgatttc ccaaaaggcc gcggtcgccg cgctgcgatt tggagagccg tttacgaaga
    11341 agatggtggc ggaattcgat cggaggcgtc aggtcattgt ggagggcctg aacaagatcc
    11401 ccggtgtctc ctgccgaatg cccggaggag cgttctatgc cttccccaat atcaagggca
    11461 tgcttggcag acgtggacca cacggtccta tcaatagccc cacggatctg gctacctatc
    11521 tactgaaaga tgcccacatt gccgtggtgc ccggggagcc gtttggcagc caacagcaca
    11581 tcaggctctc gtacgcaacg agcatggaaa tgattaagag aggcatcgac cgtttgggcg
    11641 tggcacttca gaaactcacg tgagagcttg gaccgccggc aaggcgtaac gattagctat
    11701 aaaggagaaa cgatccgtgc cgatatatga atatctctgc gaaggctgcg cctataagtt
    11761 cgaagtgaaa caaagcatga aagacgaccc catcgcgacc tgtgagcgat gcgggaagcc
    11821 ggtgcagcgc ctgatctctt cacccgctat catgtttaag ggcagcggct ggtacatcac
    11881 ggactattcg gacaagatga agccgccgac ggatggcgaa aagcccgctt cgacaagcgg
    11941 agagaagaaa gaaacgacca gtaccccagc gagccctgcg gttccggccc ctgccggcgc
    12001 ttctactccc acacaggcaa gcacgacatc cagcgccccg gcaccgtccc cgagttcttc
    12061 atcgacaacg tcttcgtccg aaaagtagcg actcccctgc acctctaccg cacgacgacg
    12121 gacttggacg cggtcttctt tttgaccgga gctttctgag gggtcttggc cgccggtttc
    12181 gtgctgctgg gtttgactgt tgcaggcttg gacgacttcg ccaagcgctc ttgcagggag
    12241 gtcaccgtgt tctgcaagct gctgatcaat gaactcttgt cgcggttcgc acggctcagt
    12301 tcgtccactt gaatctgcaa atcgtccttg accttcgtca ccgaagccag ctggttttgc
    12361 agttgggttt tttccatcgt caccgtctgc aattccgatc ggagctgctc gatttgcgtc
    12421 tgaagcgcgg gcggccaata atcacagcct gcgattccga cagcgagacc gaggatcaga
    12481 accccaccga accattgatg aacccgtctc cccatcgtca tagctccctc ctgtgtgatt
    12541 caaatggcgt agcggctggc gcctcacacc agtgaaatgc catgtgtctg tagcacgttc
    12601 tgaatcatct gtcgagtgac ggcaccgtcg cggaccactc gaaggggctg ctgcacatcg
    12661 atgaccgtcg agggcaatcc tcccgctgtc gttcccgcat cgacgatcag atcgacctcc
    12721 tgtcccaagg cctgttccac catcatggcc gtgcgtgccg gaggctgagc cgccaggttt
    12781 gcgctggtcc ccgtcagcgg cccgacgtgg gccagcaatg ccatgagcgg tttactggag
    12841 ctgagccgaa ttccaaccag accggttccg gccgtcagat tgacgggaag tgcaggcacc
    12901 gcggggaaga gaatcgtcag tggaccgggc caaaaggctt cgataaggat cttcgcaatc
    12961 gagggaacct ctcgcgccag aagcgggagt tgatccatag agccgattaa gacgaggatc
    13021 ggcttaccgt cttcccggcc tttcagacgg cgaagccgct cgactgcggc ttcgtcaaac
    13081 ggatcgactg caagtccgta gaacgtttca gtcggaatgg caacgattcc accctgataa
    13141 agccgttcgc tcacccgcct gatcaggtgc ggaagggcgt ccggagaata cgggataatc
    13201 tcggccatga gcaggaaaga aacagaaacc agccgtgagg cttatcgatt caacgcacga
    13261 tgggcaatgt ccttgcgaaa atgacagcct tcgaatgaga tcgtattcac tgcccggtag
    13321 gctcgagctt gagcttcttc cagggtcttg ccaaggccgg ttattcccaa gacacgtcct
    13381 ccagccgtca ccacgtcccc attcttcttt gccgtcccgg catgaaacac gaccgtggcc
    13441 ttatgttcgc tcgaattcaa tcccctgatg ggaaatccgc tctgatacgt tcccggataa
    13501 ccaccggatg tcatgacgac gcagaccgcg tggtcgtcgt gccactcgac cgtcaattga
    13561 tcgagtcgat gctcgacgac ggcttcagcc acgtcgatga gatcggtctt gagcaagggc
    13621 aacaccacct gcgtttccgg gtcccccata cgggcattaa actccaacac atatggcacc
    13681 cccttaacga ccatcaggcc ggcatacaac acgccttgaa acggcgaacc tgatcgcgac
    13741 atggtttcga caatcggctg caaaatctcg cgcgtcacct gttggcgaag cgtgagcgtc
    13801 ccgagaggag cgggacagta ggctcccatt ccgcccgtgt tcaagccggt atcgccatcg
    13861 ccgatccgct tgtgatcctg cgccgggagc attggagcca cggtccttcc atcggtgaag
    13921 gccatgatcg tgagctcttc cccgtcaaga aactgctcga tcagcacctg ctttcccgcc
    13981 tggccgaaca cggccttgtc catcgaatcg atgatcgcct gccgcgcggc atcgcgcgtt
    14041 gtcgcgatga tgacgccttt gccttgagcc aaaccgtcgg ccttgatcac gacgggtaat
    14101 tcatgctgtt ccacataagc cagtgcgtcg gccagcttgt ggaaatgttg agcgtgtgcc
    14161 gtcggaatct ttgcccgggc catgatatct ttcgagaaaa tcttgctggc ctcgaggcgc
    14221 gccgcattcc tggtcggacc gaagattctc agcttcgcct tacgaaactc atcggcaatg
    14281 ccgagcgcca gcggcgcttc cggccctact aacgtgagat caatctgttc ccgaatgacg
    14341 aattctctca agcctgcgat gtcgtctgct ttgatcggca cacaggccgc gagtgactcg
    14401 atgccggcat tccccggggc acagaacagt tgtggcttcc gcggactctg cgcgagtttc
    14461 cacaccatcg tgtgttcgcg cccgccgctt ccgacaacaa gaattttcat gtgtttgttc
    14521 tctgcatcaa taaaaagagg ctactcaaaa tggcctgtcg caaggccgca gcgagcataa
    14581 tgctgaatcg tgcggttacg agtacgtcga ggcgctgagc ggcgcgagaa cgaagttggg
    14641 aacctgattc agcatccgac tagtggcgga agtgacgcat gccagtcaga atcatcgcta
    14701 atccgtgctc atcagcggcc ttcacaactt ccgcatcgcg gatcgatccg ccaggctgaa
    14761 tgaccgccgt aatccccgct tgcgccgccg catcgaggcc gtcccggaag gggaaaaagg
    14821 cgtctgaggc catcacgcaa cctttcactg gcatctgcgc tttcattgca gcgagcttca
    14881 cgctgtccac acggctcatc tgccctgccc cgatcccgac cgtctgtccc ggcttcgcat
    14941 agataatcgc gttcgacttc acatgcttgc aaactttcca ggcaaacgcg caagcggcgt
    15001 actcttcatc agtcggtttt cgcaccgtcg gtaccggcaa cgcccgcaga tccgtcaaca
    15061 ccccgagatc gcgatcctga acgatcaagc cgccgacgag ctttttgagg tcgtagcctt
    15121 cctgtttcac ctttgtcagg ggaccgacat cgagcaatcg caaatctttc ttgcgcttca
    15181 actccgcgag ggcatcatcg gtaaaccccg gcgcaatcac cacctcgaca aacgtggacg
    15241 tgatctcctt cgcggcggca agatccacgg gacgattgaa ggcgatcacg ccgccgaatg
    15301 cggagatagg gtccgtctcg cgcgccttca cgtaagcttc gaccggtgtc gctcccaagg
    15361 caactccgca gggattattg tgcttgatga tggccaccgc cgtttcgtcg tattccttcg
    15421 ccaactccag cgcggaattc gcatcgagaa aattgttgta cgacatcgcc ttgccgtgga
    15481 ggatctttcc gcgcgagacg gacggctcgt tggaatgaag ctcccggtag aacgcgccct
    15541 gctgatgcgg gttctccccg taccgcaacg tctctcccag ttcatattgc aacgagagga
    15601 ttttgggaaa cttgacctcc cctccttgca tctgccgttc aagatagccg gcaatcagac
    15661 tgtcgtaccg cgccgtgtgc tgaaagactt tcatggccag ttcgcggcgg agggcatgcg
    15721 ggacgttccc cgacttggct gcatccagca cccttgaata atcggccgga tcgacgacga
    15781 ccaggacgtc ttcgtgattc tttgccgccg accgcaacat tgacggtcca ccgatatcga
    15841 tattttcaat cgcctcatcg aatgaacaat tcggcttggc gatcgtcgct tcaaaggggt
    15901 agagattcac taccacgacg tcgatcgggc cgatgttgtg ttgtctcatc tgctcgacgt
    15961 gtgccggcac cgaccgccgg ccgagcaggc caccatgaat tttcggatgc agtgtcttca
    16021 cgcggccttc gagaatttcc ggcgagccag tataggctgc gacatcggtg actttgacgc
    16081 ccgcgtcgcg caatgccttg gccgtcccgc ccgttgacag aatttccgcg ccgagggctt
    16141 ccaagccctt ggccatctca atcactccag tcttatctga cacgctgacc aacgcccgct
    16201 tgatgctggc catgattcaa ctccttggga agaggccgat aataatgtgg ttcgcgtctc
    16261 ggaaggcgag cggagaatag caaatggtgg aggacaagtt caataagaag tacggacagg
    16321 tggccgatgg ccggggcaca aaatcgctcc tcctccacgt agcccctcaa acgcttgtga
    16381 agacggtggc tcatgaagaa gacgtttcgg atctcagaca gtctcacatc ctggcttcaa
    16441 ctgaccggct tctgccgatt tcctgacccc tacaaacctg tatagagggc ctctcagggg
    16501 ctggcttgcc ccatcaagtt tgtggtaccg tcccaggtca tgtcacattg atacacgggc
    16561 tcaactcatg ggacgcagga tcattctcgg tgctggtgcg ttcgcactga ctctcctggc
    16621 cctggtgtgc attcctcgtc atcttccttc atcaaacgca tctctccctc tggtgcctgc
    16681 gaattttcac gccagactcg aacagcacga actgaccctt cgaggctctc tccctgatag
    16741 agtcacccac gaccggattc tgcaacaggc ccacagcgtg tacgaccggg cgcaggttcg
    16801 catcgtcgat caactcactg tcgatcagcg gatcgcgccg gcagcctggc tggcgacggt
    16861 cccatctatt ctccccgttc tcggagacat gaatggacgc ggatccatga tcatcgatgg
    16921 acactatctg gtcctcagcg gacgcgtacc aactgaacag gtcaagggag cgattttgcg
    16981 cagtgtggcc cccgcgacgg caaccggact ggaactggaa gaccacattc tggccgccac
    17041 ccagcctgcc tctcgtttct cattgcaggc acaattgaac gaagtcctgt cccgaaacca
    17101 gatagaattc gactcgaatc aatcgacatt gacatcccgt ggacgatccg cgctggaccg
    17161 gctgatacct ctcttgcggc aagctccacg ggcttctatc gaaatcggcg ggcatacaga
    17221 tggattcggc gcaccggatt acaaccgaga actgagccgc cgccgcgccg agaccgttcg
    17281 gcagtatttc attcaacatg gattgacgaa tcggttcacc gtcgtcggtt atggagcgag
    17341 taagcctcgg tccacggaaa tgacacagaa cgggcttcga cgcaaccgac gtatcgaact
    17401 gcgcgtgcaa gaacagggtg aactatgatg gtgttgatcg gccaaatggt gggatgtctt
    17461 ctcgtggcag ccgcaatcgg aggggccgtg ggctggctgc tccggcacct ctcggccgca
    17521 ccaatcgagc agcggctgat cgactccgaa acggacttgc gtatcaaggg gcaggctctc
    17581 gacacggccc tctacgaatt acgagtgaac aagtccacac tggccacgct cgaggcgaaa
    17641 ctctcctcaa tggaaagtct cggacgatcc acacagcaag aacttacctc acgccaggac
    17701 cggatccaga cccttcaaaa ggaactggcc aactcacagc aacggctgtc ggctatggaa
    17761 tccgaccaca aggcctccct ccaacgcctg acggaacagg agaccgccat ggcggcgttc
    17821 gccaacgaag ccaggcgggc gaatgcggct cgaacggctg cgcaacaaga cctccttctg
    17881 aaggaacaat cagccagcga gcttcagcaa cgactcactg aattggaaga tcagcaggtt
    17941 gaaatcgacc ggctccgcgc acaggttgcg gaattggagc cggttcaagg acgccttcat
    18001 tggatggaag tccagctcag cgagaaagag acccagcacc gggccaatct ccatcgattg
    18061 gaagagcagc tcgcatttcg cgatcgccgc attgccgagc tggcggagct tgaacaggct
    18121 ttgcgcgaga aggatctgcg catcgccgaa ctcgagcaac gtctcgccga agccgatgtg
    18181 ttgcacacag agctcgccgg ccaggcgaaa atgatgggag aaaaagaaga ggaaatttcc
    18241 cgccttcgaa agcgcctcgt ggaagtccgc gccgccctcc gcgttcgcgc cgacggcggc
    18301 catgttgtca ccggtcgtgc caattctacc aatcagctca gtctgcagat cgagcattcc
    18361 aaacagaccc agaagccgca gaaagacgat ttgaaaaaga ttcacgggat cggcccggtt
    18421 atcgaacgca ctttgaacaa actgggcacc tacacatacg tgcagatcgc gaagtggacg
    18481 cccagcgaca tcgcgcgtgt cgcgcagaag ctggagacca tccccgatcg tatcaagcgc
    18541 gacaattgga ttgcgagcgc gagaaagcta tacaaagaga aatacggcga acgaatctaa
    18601 gcggtagttg ccccgttccg ggttcattga cttccccttc tcggcctcct aaaatcgccc
    18661 atggcttcat ccttcgttca tcttcatctc cacacccaat tcagccttct cgacggcgcc
    18721 aatcagatcg agccgctggt gcagcaaatc aaggcgttcg gccaaccggc cgtggccatg
    18781 accgaccacg gcaacatgtt cggcgcgatc gagttctatc gcaaagccaa ggaagcagga
    18841 gtgaagccg
//
LOCUS       DATIFR010000125        13086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_848809, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000125 DATIFR010000000
VERSION     DATIFR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848809"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VLD60_07230"
     CDS             complement(<1..629)
                     /locus_tag="VLD60_07230"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSB44785.1"
                     /translation="MRVVQTHNKHVVFGILLSALIVGVMLTIGQVPLAVSQPVTIPAK
                     AVKGPIPMDGANPIWESVPGVIVPLSGQLITTPMHPNISVKSVFVKAMTNGKELGLRV
                     EWSDQTKNDTTIGPQDFRDQAAIMFPVNTSGAPPFQCMGQSGGTTNIWRWNAEWQKDL
                     GKDSAGIWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNPGIWSG"
     gene            complement(728..1699)
                     /locus_tag="VLD60_07235"
     CDS             complement(728..1699)
                     /locus_tag="VLD60_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HSB44786.1"
                     /translation="MTSKQPVPSSTQAATAVIPNPPAIKDSPAVERALNRSKLYLLVS
                     WSLLYPEDEEFLDYLQCGEFVEDGRAALDALEVALDGMGGERAKQKLALMRKQLEQIE
                     KLVASECVNWQLGDLQAEHRRVFSNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFYK
                     AFGVELSKDIHERLDHLSVEFEFMHFLAYKESYSRCHDGADKTQIVVDAQKKFVKNHI
                     GRWVPLFCRMLIKKADSGLFKHVADMTSDWMDFEAAYLGVTPQPYSEADYRPATFNSP
                     EGQTYECGAQDQGNELSLLLNETGTQNFMDQKDKEKDKEEGGPIGTA"
     gene            complement(1935..2534)
                     /locus_tag="VLD60_07240"
     CDS             complement(1935..2534)
                     /locus_tag="VLD60_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB44787.1"
                     /translation="MASDPKYGRRDFLKDSVLSVAKAAQEFSKHKEVVPDKPAPVLRK
                     DWLRPPGAVEESLFLERCTKCNDCVKACPHGSIVFHQRDGTPVIFPDQVPCYLCEDLP
                     CIAACATEALIPVEGPEQVRMGVATVSHRVCTAGQGCHACVSKCPTDALSMDFDAQRL
                     VVTAERCVGCGVCEQICRTVNDHIAIRVTPIRLLEPLAP"
     gene            complement(2551..3549)
                     /locus_tag="VLD60_07245"
     CDS             complement(2551..3549)
                     /locus_tag="VLD60_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44788.1"
                     /translation="MEIRFQPALLQEVIDSFVEKTEREGDPTYYKEFHEIADPIYEKF
                     TLDDRESEFKKLYQYLFGTWGFSDIIRDAFNEYPILKKQIGIVLVKGVLKEDQEGVDI
                     LRKWGSVEQDLARQFEDKGLKGVGIKLIPRRFYDPALTRYCRHELMHISDMVDPLFGY
                     DPDTKVGQNPGEETLIHHRYRILWSLSVDSRLTAAGKEPMLKKEDRFKEFRSWYRKIP
                     APQLKSVFEGLWQTGFFTHSELIEMATDTLRVMDRAVDVEGGEVPETENKVMLMPGFP
                     CPLCRFPTYSWVEDLGNKIETYVLDFIRENHPGWDVEFGACDRCVEVYKLRADGVM"
     gene            3767..4867
                     /locus_tag="VLD60_07250"
     CDS             3767..4867
                     /locus_tag="VLD60_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HSB44789.1"
                     /translation="MADQTASPLAPAVASPASASETPAPLTDHLEYWKAGARELNTFR
                     GHSHGVWAVAFAPDGLTLASAGVDRLVRIWDIETGRLLRSMRGHTNDIRAIVFTPDGQ
                     TLATGSEDRTIRLWNPKTGEPMKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIKIWE
                     VTTGTELMTLLGKDQYDHQWSVCVAFSPDGIHLASGADIGKIKIWEVLPSGEEKILHN
                     GHWRQDNEDTTETRGYFIEDDGGFQKPMDYWIGAMTFTHDAKYLITGSRDQTIKMFEM
                     PQVLEHRTLKGHSGWIRSLVVTPDNKVLISSADDNTIRFWDLATGRSFRTIKGHSGGV
                     RCISLSPDGRRLASASWDRTVKLWEGGSEATE"
     gene            complement(4904..6343)
                     /locus_tag="VLD60_07255"
     CDS             complement(4904..6343)
                     /locus_tag="VLD60_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB44790.1"
                     /translation="MKGLAILLVDDEPLMRLSMVDALEAVGYDVHAASSGTEGIEAIR
                     RRAFDLVITDLRLPGADGLTVLKATKEQSPQTEVVVITAHGSVESAVGAMKLGAFDYI
                     TKPFQMDELLLIVERVGRVVALRRENQDLKETLENKFSFNGILGTNSQMRAVLDKIKL
                     VAGTDSTILILGESGTGKELVANAIHQNSARSQYPLIKVSCAALPETLLEAELFGHEK
                     GAFTGAIRQRRGRFELAHRGTLFLDEIGEISPVVQVKLLRVLQERKFERVGGNDTIDA
                     DVRLVCATQKDLRKEVAQGRFREDLFYRLNVVQVVIPPLRKRQEDIMVIAEHVLQVCS
                     TKMNKKLKGFSPASREVLLRYSFPGNVRELENMVERGVALGREREAVQPSDLCGFQSC
                     PFTGGVPQESCGFCSEGLTGQKRPDRPMTSLAAAREEFEKDYIVSVLERVEGSRTTAS
                     KILGLSRKALWEKCKRYGIPSAKDEPEEE"
     gene            complement(6340..9525)
                     /locus_tag="VLD60_07260"
     CDS             complement(6340..9525)
                     /locus_tag="VLD60_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB44791.1"
                     /translation="MNLWSRSHSLQHKIITAIVMVGLLPLTLLLALTYVEERRALRET
                     TGTNFKEVAVEAARRIEMHVSRGMNEAQQLATTPFLRTSVTEANRTYEGKGPQGIQDM
                     IKDWQQRWKQRDKRSEFPLFINRIVTNYLIRWHDIRKSDYVGILVTDAEGALVVSSIP
                     QVDYYYGKTAWWRAVVKGGNREAYVSEITFDPAFGTHVVVVAAPIFDDIQKSVIGAVT
                     ILLRRDTLFHSIAEVSIGSTGHAMLFSSDGVPVICPILAPEEHSINAELVNILGALKA
                     GWAVAADDSHGSHNALIGFAPVRFSDRLAPGSIGGKQWITVVRQDPQETFAPLADLVA
                     KVLLYGLAVLGVLWGTGVLVARRIARPIQLLHDGVQEIGSGRLEQRLELKTGDEIERL
                     AEAFNQMAANLQRSFGQIEQRMADVHRLEEKYRDLIEHSPEMIYQLNRSGQFVHVNKT
                     GLDKLGYSLDEMLAMRLWDLVPRGQESPVLHYLEQLMSQGQSSMETVFLAKDGHPIDV
                     EIHATALFDEERGGLVHSRAFVRDVTERHRLEQELQHYTSGLEQAVSERTQQLVASQA
                     RYKALFDLVADSVFMVDATGTVVAVNKREEQALGYAESDIVGRSIHDVVVPAHRQALA
                     GWLADIGTGKRQVPTQEITVFNAAGLDTPVEMDLIRIGSADQLLVMVQLRDITDRKKL
                     ERQLQTYREELEVKVRERTREIEETKQYLENLLENANDVIYTLDTDQRFTYVNSKVEV
                     WGYRKDDLLGRPYLSLLSRRHRGRRLKNTLDIGAKQVYEVEVVTRMGEIRTVMVSVSP
                     LQGVDGEILGVLGIARDMTETKKLEQQIRNSEKLASVGKLAAGVAHEINNPLGGILNC
                     LYNLRKGALSPARQEEYWASMEDGVRRVQKIVRQLLDFSQQHEPEFSPTDINQVVDRV
                     LVLTNHLFAPNRIVLETELGHGLPSLMVDRHMIEQVMMNLILNAVQAMKSGGILTIRT
                     SVAEGICRVEVGDTGSGIPPAVLPRIFDPFFTTKSEGEGTGLGLSVSLGIVERHGGKI
                     LVESEVGKGTTFTLCLPLSRERSLVEREKVS"
     gene            complement(9580..10497)
                     /gene="secF"
                     /locus_tag="VLD60_07265"
     CDS             complement(9580..10497)
                     /gene="secF"
                     /locus_tag="VLD60_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSB44792.1"
                     /translation="MLEILGKTNIDFMGKRRYAFLFSGIMVLLGIIGLIQIGRGAANL
                     GIDFAGGTAVQLKFDQPVRIDEARKALEANGLGDAELQEFGQDNKLLIRVKASTTIEE
                     KTAERVVVIFTKEFPANKFVVDSTTEIGPTIGKKLQEDALIAVLVSFAGIILYIAARF
                     EFRFGIAAALATFHDVLAVLGSFYVLDKEITLLVVTALLTLAGYSLTDTVVVFDRIRE
                     NLRTRRRESEETMINNAINQVLSRTIVTSLTVVLVLIPLTIAGGEVLHDFSLALLWGV
                     IFGTYSSIFVASPLLLLWPVTSGRLLKRS"
     gene            complement(10575..12218)
                     /gene="secD"
                     /locus_tag="VLD60_07270"
     CDS             complement(10575..12218)
                     /gene="secD"
                     /locus_tag="VLD60_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSB44793.1"
                     /translation="MKRVRGRLTLLVLVLVVSAVFFLPSYQPLYQALPGWAKSVLPNK
                     GITLGLDLQGGIHMVMEVDEDRAVEIAVDRSVVSLQDMLVDKKIPAESVKRTGPTRIT
                     IEFQNADLKAQIQKLIDEFPTFTETDSAGSANMLVWDLRETEAKRIKDSAINQALETI
                     RNRIDQFGVAEPLVQRQGLKQIVVQLPGVKDPKRAKDLIKETALLEFKMLDEENQMKL
                     DLPARISKDKEADVVKQVEGKLPEGDQILFERAIDKDTGREYRIPYLVKKRVMLTGDV
                     LSDARVSIGQFNDPYVSITFDAKGGREFERITGDNVKKRMAVVLDNTIYSAPVIQERI
                     TGGRAQITGTFSTQEANDLAIVLRAGALPAPLKIIQDLTVGPSLGQDSIEKGVKATLV
                     AGAMVVIFMIIYYRLSGVIADFALVLNLICLMGALSALTATLTLPGIAGIVLTIGMGV
                     DSNVLIFERIREELRSGKAVRLAVDGGYDKALLTIIDSHVTTLITGVALFLFGTGPIK
                     GFAVTLCLGIAINLFTALVGTKVIFDVINQRHKVEQLSI"
     gene            complement(12249..12581)
                     /gene="yajC"
                     /locus_tag="VLD60_07275"
     CDS             complement(12249..12581)
                     /gene="yajC"
                     /locus_tag="VLD60_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSB44794.1"
                     /translation="MFMESVAWAQGTGGGGSGAGTLLSLVPFVLIFVIFYFLLILPQQ
                     KKQKQQKAMLEALKKGDKVITASGIWGTVTNLGKETVTLQIADNTKIKMQREFISRVR
                     ADEEDKES"
     gene            complement(12603..>13086)
                     /locus_tag="VLD60_07280"
     CDS             complement(12603..>13086)
                     /locus_tag="VLD60_07280"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSB44795.1"
                     /translation="TVPELPVSKPRYLMGVGMPENLIEGVARGIDLFDCVVPSRHGRT
                     GWLFTSFGRVLIKQARYQRDEQPIDPTCGCPVCARYSRAYLHHLYHMKEMLASRLNTI
                     HNLWYFADFMRRMRVAIGQGTFSEFREAFYRAQEREAPEVAALANREAGTIRRTALDV
                     "
BASE COUNT         2804 a         4020 c         3417 g         2845 t
ORIGIN      
        1 cccgaccaaa tgccggggtt gaagggccca aggctgcgac cgatccggtc cggataggtc
       61 acaccgcccg cgggctcttc gaaatagtag tcccagaaaa tgccgggata ttgatcgtcc
      121 acgtcccaga tcccggcgct gtccttgccg agatccttct gccattccgc gttccaccgc
      181 cagatgttgg tggtcccgcc ggactggccc atgcactgga acggcggcgc gcctgacgtg
      241 ttgaccggga acatgattgc ggcctggtcg cggaagtctt gcggaccgat cgtcgtatcg
      301 ttcttcgtct gatcgctcca ctccacacgc agcccgagtt ccttgccatt cgtcatggcc
      361 ttgacgaaca ccgacttgac ggagatgttc gggtgcatcg gcgtggtgat cagctgacca
      421 ctcaacggca cgatcacacc aggcacactc tcccaaatgg ggttcgcccc atccatgggg
      481 atgggaccct tgaccgcctt cgccgggatg gtcaccggct gactcacggc caacggcacc
      541 tgcccaatcg tcagcatcac gcctacgatc agggcagaga gaagaatgcc gaacaccaca
      601 tgcttgttgt gtgtctgcac tactctcatg gactatgccc tcctctcgat taagttatta
      661 agaactgtga cccataatga ctgccgacaa acaaaaaatg gaaacaccgg acatgaatac
      721 cgattcgtta ggcggtcccg atcggccccc cttcctcctt gtctttctct ttgtccttct
      781 gatccataaa attctgcgtg cctgtctcgt tgagcaaaag actcagctca ttaccctggt
      841 cctgcgcacc gcactcatag gtctggcctt ccggagaatt gaacgtggca ggacgataat
      901 ccgcttccga gtagggttgc ggggtcacgc ccaagtaggc agcttcgaaa tccatccagt
      961 cggacgtcat gtcggccaca tgcttgaaga gcccggaatc cgctttttta atcagcatgc
     1021 ggcagaacag cggaacccag cgcccaatgt ggttcttcac gaacttcttc tgggcatcga
     1081 cgacgatctg ggttttatcc gctccatcgt ggcagcggga ataggattct ttataggcga
     1141 ggaaatgcat gaactcgaac tcgacgctca ggtggtccag ccgctcatgg atatccttgg
     1201 agagttcgac gccgaaggcc ttatagaagc ccgcgatgtc ccccatgacg tgagattgtg
     1261 cgaagacgtg gtcgttacca aagagcgttt cgtagggagg acaatcgagc gtgatgacat
     1321 tgctgaacac gcggcgatgt tccgcctgca gatcgccgag ttgccaattc acgcattctg
     1381 atgccacgag cttttcgatc tgctcgagct gtttgcgcat cagcgccagc ttttgcttgg
     1441 cacgctcacc acccattccg tcaagcgcga cttccaatgc atccagcgcg gcccgaccgt
     1501 cttcgacaaa ctctccgcac tgcaggtagt cgaggaattc ttcgtcttcg ggatagagca
     1561 ggctccacga cacaagcaga tagagcttgc tgcgattgag tgcacgttcg actgcgggag
     1621 aatctttgat ggctggggga ttcggaatga cagctgttgc ggcttgtgtg gaacttggta
     1681 ccggctgctt actcgtcatg agaatcagct cctcggttca acccgatacc acgacactct
     1741 tcggcatcgg tggtcaaacc agtggcagaa gtttgggtat gataggtaaa ggtcttgggg
     1801 atgtcaagca tgatctcgtc ccccaaatac aaaagatgac tggctgaatt gtcataccat
     1861 tgatcgtcca tgttcctccc gttttttcct tgacttctcc atcatcttgg cccatttcgg
     1921 ccagctggat ctggttacgg agccaggggt tccaacaggc gaatcggggt cactcggatc
     1981 gcaatgtgat cgttcaccgt ccggcagatt tgttcacaga ctccgcatcc aacacaacgt
     2041 tcagccgtga ccacaagtcg ttgcgcatcg aaatccatcg agagcgcgtc cgtcggacat
     2101 ttcgacacac aggcatggca accctggcct gcagtgcaaa cacgatgaga tacggtcgcg
     2161 acgcccatcc gtacttgttc tggcccctcg acgggaatta acgcctcggt ggcacaggca
     2221 gcaatgcaag gaagatcctc gcacagataa cacggaactt gatcagggaa tatcaccggc
     2281 gtgccgtcac gctggtgaaa gacgattgat ccgtgaggac aggccttcac acagtcgttg
     2341 cacttcgtgc agcgctcgag gaataacgat tcctcgacag cgcctggcgg acgcagccag
     2401 tccttgcgca gcaccggcgc cggcttatcg ggaacgactt ctttgtgttt ggaaaattcc
     2461 tgcgcggcct tcgcgaccga gaggaccgaa tctttgagga aatcacggcg cccgtatttg
     2521 ggatcgctag ccacgggaaa ggtacccccg ttacatcacg ccgtcggcac gcagcttata
     2581 gacctcgaca caacgatcgc aggcgccaaa ttcgacatcc catcccggat gattctcgcg
     2641 gataaaatcc aggacgtagg tttcgatctt gttgcccaga tcttccaccc aggaataggt
     2701 cggaaatcgg cagagcggac aggggaagcc cggcattagc atcactttgt tttccgtttc
     2761 gggcacctcg ccgccttcaa cgtcaacggc gcgatccatg acccgcaacg tatctgtcgc
     2821 catttcaatc agctcagaat gcgtgaaaaa tccggtttgc cagagccctt caaataccga
     2881 cttcaactgc ggcgctggaa tcttgcggta ccaggaccgg aactccttga accgatcctc
     2941 cttcttgagc atcggctcct tgcccgccgc cgtcagccgg ctatccacgc tgagactcca
     3001 caagatccgg tagcgatggt ggatcaaggt ttcttcgcca gggttctgcc cgaccttggt
     3061 gtcgggatcg tatccgaaca gcggatcgac catgtcggag atgtgcatca attcatgacg
     3121 gcaatagcgc gtcagcgccg gatcgtagaa cctccgggga atcagcttaa tgcccacgcc
     3181 tttcaacccc ttgtcctcga actgccgggc gagatcctgt tcaaccgatc cccattttct
     3241 tagaatgtcg acaccttcct ggtcttcctt gagcacgcct ttgaccaata caatcccgat
     3301 ctgcttcttc agaatggggt actcgttgaa ggcatcgcga atgatgtccg aaaatcccca
     3361 ggttccaaaa aggtactggt agagcttctt gaattcgctt tcccggtcat ccagggtaaa
     3421 cttctcatag atggggtcag caatttcgtg gaattcctta taataggtcg gatcgccctc
     3481 ccgttcggtc ttctccacga acgagtcgat gacctcctgt aataacgccg gctgaaagcg
     3541 aatttccatg acgatcctca atgctccgat tcagaagtgg cagccactcg tttcgatcaa
     3601 ggcccggtac aaaacggctc tcctcgcggc ttgacttgat ggccaaggtt ctcttacgat
     3661 ccacccgata ccaaatcgaa ttatactggc ccgaaacggc gatttgcaat caagcccatc
     3721 gccgtaggtc gctttaccct gtgtgatgtt cggaaggaca gagactatgg cagaccaaac
     3781 cgcttctccc ctcgcaccag ccgttgcttc accggcctcg gcatccgaaa caccggcccc
     3841 gctcaccgat caccttgaat attggaaagc gggagcacgc gaactcaata cattccgagg
     3901 ccattcccac ggagtctggg cagtcgcctt cgcaccggat gggttgacac tagccagcgc
     3961 cggcgttgac cgactcgtcc gcatttggga tattgaaacc gggcgattgc ttcgctccat
     4021 gcggggccat acgaacgaca ttcgtgccat cgtctttacg cccgacggac aaacccttgc
     4081 aaccggaagc gaagatcgga cgatccggct ctggaatccc aaaaccggcg agccaatgaa
     4141 actccttttc acacgctatg atcataacgt ctgcagcctt tcgctctcac ctgatggctt
     4201 gatgctggcc cgcggcagcc acaataagga catcaaaatt tgggaagtca ccaccggtac
     4261 ggaactgatg acactactcg gcaaagacca gtatgatcac caatggtcgg tctgtgtcgc
     4321 gttttcaccg gatggcattc atttggccag cggagccgac atcggcaaga tcaagatctg
     4381 ggaagttctg ccgagcggtg aagaaaagat cctgcacaac gggcattgga ggcaggacaa
     4441 cgaggatacc actgaaacac gcggatactt tatcgaagac gatggcgggt tccaaaagcc
     4501 gatggattat tggatcggcg ccatgacctt tacccatgat gccaaatacc tcatcaccgg
     4561 aagcagagac cagacgatca agatgtttga aatgcctcag gtcctcgagc accgaacgct
     4621 caagggtcac agcggctgga tccgctcgct cgtcgtgaca cccgacaaca aggttctcat
     4681 cagttcggcg gacgacaaca ccattcgctt ctgggatctc gccaccggcc gctcgttcag
     4741 aaccatcaag ggacatagcg gcggcgtccg atgcatctcg ctctcaccgg atggacgacg
     4801 gctcgccagc gcgtcgtggg atcgaaccgt aaagctatgg gaaggcggct cagaagcgac
     4861 cgagtaattc tcgcaagctg cgggattttt cagcagccgg ctgctactct tcttccggct
     4921 cgtctttagc ggatggaatc ccgtagcgtt tacacttttc ccacaaggcc ttgcgagaga
     4981 gccccaggat cttcgaggcg gtggtacgac tgccttcgac tcgctccagc acggaaacaa
     5041 tataatcctt ctcgaattct tcgcgggccg cggccaacga cgtcatcggg cgatcggggc
     5101 gcttctgtcc cgtcaaccct tcgctgcaga atccacatga ctcctgaggg actccgccgg
     5161 taaacgggca ggactgaaag ccgcacaggt cggacggctg cacggcttcg cgttcacgac
     5221 ccagcgccac tccccgctcc accatattct ccagttcccg cacgttgccg gggaaggagt
     5281 accgcagcaa gacctcgcgc gaagccggtg aaaatccctt gagtttcttg ttcatcttgg
     5341 tcgagcagac ctgcaagaca tgctcggcga tgaccatgat atcttcctga cgtttgcgta
     5401 agggcggaat cacaacctgg acgacgttga gccgatagaa taggtcttcg cggaaccgtc
     5461 cttgtgccac ttcttttcgt aaatccttct gcgtggcaca cacaagacgg acgtcggcgt
     5521 cgatcgtatc gtttcccccc acacgttcga atttccgttc ctgcaaaacc cgcagcagtt
     5581 tgacctggac gacgggagag atctctccga tttcatccag aaacaaggtg ccccggtggg
     5641 ccagttcaaa ccgccctcgg cgctggcgga tcgctccggt gaatgcccct ttttcatgtc
     5701 cgaacaattc cgcttccagt aaggtctccg gcaatgccgc gcaacttacc ttgatcaaag
     5761 gatactggct tctggcgctg ttctgatgaa tcgcgttggc aaccagctcc ttccccgttc
     5821 cgctctcgcc cagaatcagg atggtcgagt cggttcccgc taccaattta atcttgtcga
     5881 gcacagccct catctggctg tttgtgccca ggatgccgtt gaagctgaac ttgttctcca
     5941 acgtctcctt gagatcttgg ttttcgcgcc gcaacgccac cacgcggccc acgcgttcca
     6001 cgatcagcag gagctcatcc atttgaaacg gtttggtgat gtaatcgaag gcgccaagct
     6061 tcatggctcc gaccgctgat tcgaccgacc cgtgcgctgt aataaccacc acttcggttt
     6121 gaggcgactg ttcttttgtc gctttcaaca ccgtcaatcc atccgcaccg ggtaaccgga
     6181 gatccgtgat caccaaatcg aaggccctcc gacggatcgc ttcgatcccc tcggtccctg
     6241 acgacgccgc atgcacgtcg tagccgaccg cttccagggc gtcgaccatc gagagtcgca
     6301 tgaggggctc gtcatcaact agcaggatgg cgagaccttt catgacactt tttcccgttc
     6361 caccaacgaa cgctcgcgcg acaacggcag gcataaggta aacgtcgttc ctttcccgac
     6421 ttcgctctcc acgaggatct taccaccgtg gcgttcgaca attcccaagc tgacggaaag
     6481 ccccagtccg gtgccctccc cttcgctctt cgtcgtgaaa aacggatcga agatgcgcgg
     6541 caggaccgcc ggtggaatac cggatcccgt atcaccgacc tccactcggc aaatcccctc
     6601 ggcgacggaa gtccgaatcg tcaagatccc tccgcttttc atggcttgga ccgcgttcag
     6661 aatcagattc atcataactt gctcgatcat gtgccggtcc accatcaagc tcggcagccc
     6721 gtgacccaat tctgtctcca gcacaatccg attgggagca aacagatggt tggtcagaac
     6781 cagcactcga tcgacgacct ggttgatatc ggtgggactg aactccggct cgtgctgctg
     6841 agagaaatcg agtaactgcc ggacgatctt ctgcacgcgc cgcacgccgt cttccattga
     6901 ggcccaatat tcttcctggc gggccggcga caaggcaccc ttgcgcaggt tgtagaggca
     6961 attgagaatt ccacccagcg gattgttgat ttcatgggcg acaccggccg ccagctttcc
     7021 gaccgaggcg agtttttccg agttcctgat ctgctgttcc aacttcttcg tttccgtcat
     7081 gtcgcgcgca atgccgagaa cacccagaat ctctccgtcc acaccctgaa gaggggagac
     7141 gctcaccatg accgtgcgta tctcgcccat gcgggtcacc acctcgacct cgtagacctg
     7201 cttggcgccg atgtccaagg tattcttgag gcgccggccc cggtgacgcc tcgagaggag
     7261 cgaaaggtag ggccgaccga ggagatcgtc cttccgatag ccccacactt cgaccttgct
     7321 gttcacatag gtgaaacgct gatcggtatc gagcgtatag atcacatcgt tggcattttc
     7381 gagcagattt tcaagatact gttttgtctc ttcaatttct ctcgttcgct ctcgcacttt
     7441 gacttcgagt tcttcgcgat acgtctgaag ttggcgttcg agcttcttcc ggtcagtgat
     7501 atcgcgcagt tgcaccatca ccaagagttg atcggcggag ccgattcgga ttaagtccat
     7561 ctcgaccggg gtgtcgagcc cggccgcgtt aaagaccgta atctcctgag tcggcacctg
     7621 ccgcttcccg gtaccaatgt cggcaagcca ccctgccagg gcttggcgat gcgccggcac
     7681 aaccacgtcg tgtatgctgc gccccacgat gtccgactcg gcatagccga gcgcctgctc
     7741 ctcccgcttg ttgaccgcga cgacagtccc tgtcgcatcc accatgaaca cggaatccgc
     7801 gaccaaatcg aatagcgctt tgtagcgtgc ctgcgacgcc accaactgct gtgtccgctc
     7861 cgagaccgcc tgttccagcc ccgacgtgta gtgctggagt tcctgctcca atcgatggcg
     7921 ttcggtcacg tcgcgcacga aagcgcgcga atgcacgagc ccgccccgct cctcatcaaa
     7981 gagagcggtc gcatgaattt cgacgtcgat gggatgcccg tccttcgcca ggaagacggt
     8041 ctccatagag ctttgccctt gcgacatgag ctgttcgagg tagtgcagaa ccggcgactc
     8101 ttgtccgcgc ggcaccaggt cccagagcct catggccagc atttcgtcga gggaataacc
     8161 cagcttatcc agcccggtct tattcacgtg cacgaactgg ccgctgcggt tcagctgata
     8221 gatcatttcg ggggaatgtt cgatcaaatc ccgatacttt tcttcaagtc gatggacatc
     8281 cgccatccgc tgctcgatct gaccaaagga gcgctggagg tttgccgcca tctggttgaa
     8341 ggcttcggcg agtcgttcga tttcatcgcc ggttttgagt tccagccttt gttcgagtcg
     8401 gccgcttccg atttcctgca cgccgtcatg caggagttga atcggacggg caatgcggcg
     8461 tgcgacgagg acccccgtcc cccacaggac acccagcacc gccaacccgt acagcaacac
     8521 cttggccaca agatcggcga gaggcgcaaa cgtttcctgc gggtcctggc gaaccaccgt
     8581 gatccactgc ttccccccga tactgcctgg cgcaagccgg tcgctgaacc ggacaggggc
     8641 aaatccgatg agggcattgt gactcccgtg cgaatcgtct gccgccaccg cccatcccgc
     8701 tttgagcgca ccgaggatat tgaccaattc agcattgatc gaatgttctt cgggagccag
     8761 gatcggacag atcaccggga cgccgtcgga gctgaagagc atcgcgtgac cggtcgaacc
     8821 aatcgagact tcggcaatcg agtgaaacag cgtgtcgcga cggagcaaaa ttgtcacggc
     8881 accgatcacg gatttctgta tgtcgtcgaa aatcggcgcg gccacgacaa cgacatgggt
     8941 tccaaacgcg ggatcaaacg taatctcgct gacataggcc tcccggttcc ctccctttac
     9001 caccgcccgc caccaggcag tcttgccgta gtagtagtcc acttgcggga tggaactgac
     9061 caccagcgcc ccctccgcat cggtgacgag aataccgaca tagtcggact ttctaatgtc
     9121 atgccatcgg atcagatagt tagtcacgat ccggttgatg aagagcggga actcactgcg
     9181 cttgtctcgc tgcttccagc gctgttgcca gtccttgatc atgtcttgga tgccttgcgg
     9241 acctttgccc tcgtacgtgc ggttggcttc cgtgacggag gtgcggagaa acggcgtggt
     9301 ggcgagttgt tgcgcctcgt tcatcccgcg gctgacatgc atctcgatcc ggcgggcggc
     9361 ttctacggcg acttctttga aatttgtccc cgtcgtttcg cgcaaggcac ggcgctcttc
     9421 gacgtaggtc agggcgagta ggagcgtgag cggcaggagt ccgaccatca cgatcgccgt
     9481 gatgatcttg tgctggagac tgtgggaccg actccagagg ttcatgggta ttcgcacgag
     9541 ggccgattag gatcggcgcc tcgactccgc cccatcgggc tagctacgct ttaacagtcg
     9601 accggaggtt accggccata acagcaacaa cggactggcg acaaaaatgg acgaataggt
     9661 cccgaatatc acgccccaca aaagggctag cgaaaagtca tgcagcactt cccctccggc
     9721 aatagtcaac ggaatcagga ccagcaccac ggtcagactg gtgacgatcg tccggcttaa
     9781 gacctgattg atcgcgttgt tgatcatcgt ctcctcactc tcccgacgcc gcgtgcggag
     9841 gttttcgcgg atacgatcga agacgaccac ggtatccgtc agcgaatagc ccgccagggt
     9901 caacaatgcc gtcacgacca agagggtgat ttctttgtcc agcacataga aggaccccaa
     9961 caccgccaac acgtcgtgaa aagtcgccaa ggcggctgcg atcccgaagc gaaactcgaa
    10021 ccgcgcggcg atgtacagga tgatgccggc aaacgagacg agcacggcga tgagcgcgtc
    10081 ttcctgaagc ttcttgccga tggtcgggcc gatttccgtg gtggaatcca ccacgaattt
    10141 attcgcggga aactccttcg taaaaatgac caccacgcgc tccgcggtct tctcttcgat
    10201 ggtcgtggag gctttcacgc gaatcaacaa cttattatcc tggccaaact cctgcaactc
    10261 ggcatcgccg aggccattgg cctccaaggc cttcctggcc tcatcaatcc gaaccggctg
    10321 atcgaatttt agctgcaccg cggtcccgcc cgcaaagtct atgccgagat tcgccgctcc
    10381 ccggccgatc tgaatcaatc caataatccc cagcagtacc atgatgccgg agaagagaaa
    10441 cgcataccgg cgtttcccca tgaaatctat attcgtcttc ccgagaattt ctaacatgcc
    10501 tactcccttg ctaacagcga tcggccctca gcgatccgca agccgcggaa cgcccgtcgg
    10561 cgatggcgtc gtggctaaat gctcaattgt tccactttat ggcgttgatt gatcacgtcg
    10621 aaaatcactt tggtgccgac caacgcggta aacaagttga tcgcgatgcc caggcagagg
    10681 gtcacggcaa accccttaat cggaccagtc ccgaagagaa acagggccac accggtgatg
    10741 agcgtcgtga catgggaatc gataatcgtc agcaacgctt tgtcataacc accatccacc
    10801 gcgaggcgga cggctttccc gctccggagt tcctcacgaa tacgctcgaa gatcagcacg
    10861 ttcgagtcga cgcccatgcc gatcgtcagc acgatgccgg cgatgccggg caacgtgagg
    10921 gtcgcggtaa gcgccgacag ggcgcccatc agacagatca agttgagaac aagggcaaag
    10981 tccgcaatga caccggacag cctgtaatag atgatcatga agatgacgac catcgcgccg
    11041 gctacaagcg ttgccttcac gcccttttca atcgaatcct gtccgagcga tggtccgacc
    11101 gtcagatctt gaatgatctt gagcggtgcc ggcaaggcac cggcccgcag cacaattgcc
    11161 aagtcgttcg cttcctgcgt ggagaatgtc ccggtgattt gtgcgcgacc accggtaatc
    11221 cgctcctgaa tgaccggagc cgagtagatc gtgttgtcga gcaccaccgc catgcgcttc
    11281 ttgacattgt cgccggtgat ccgctcgaac tcccgcccgc ccttcgcgtc gaacgtgatc
    11341 gacacatagg gatcgttgaa ttggccgatt gagacgcggg catcgctcaa cacatccccc
    11401 gtcagcatca ctcgcttctt gacgagatag ggaatgcgat attcccggcc cgtgtccttg
    11461 tctatcgccc gctcgaagag gatctgatct ccctccggca acttgccctc gacctgcttt
    11521 acaacgtccg cttctttatc cttggaaata cgcgccggaa gatccagctt catctggttc
    11581 tcttcgtcga gcatcttgaa ttcgagtaaa gccgtctcct tgataaggtc cttggcccgc
    11641 ttgggatcct tgacgcccgg cagttggacg acaatctgct tcaatccttg gcgctgaacc
    11701 agcggctccg ctacgccaaa ttggtcgatg cggttgcgaa tcgtttcgag cgcctggttg
    11761 atggccgaat ccttgatgcg cttcgcctcc gtctcccgca aatcccacac cagcatattc
    11821 gccgagcctg cagaatctgt ttccgtgaac gttggaaact catcgatcaa cttttgaatc
    11881 tgtgccttga gatcggcgtt ctgaaattca atcgtgattc tcgtcggccc tgtgcgcttg
    11941 acggattcag cggggatctt cttgtccact aacatgtcct ggagcgacac gaccgagcga
    12001 tcgacggcga tttccaccgc acgatcctca tccacctcca tcaccatatg gatcccaccc
    12061 tgcagatcca agcccagcgt gatgccttta ttcggcaaca ccgatttggc ccagccgggc
    12121 aaggcttgat acagtggttg gtacgacggt aaaaagaata ccgccgagac caccagcacc
    12181 agcaccaata acgtcagccg cccacgtact cttttcatag cgcttccaac ccctttgtcc
    12241 tgtgcagact acgactcctt gtcttcttcg tccgcgcgca cccgtgaaat gaactcccgc
    12301 tgcattttga tcttcgtatt gtcggcaatc tgaagcgtga ccgtctcttt ccccagattg
    12361 gtcacggttc cccaaattcc ggaggccgtg atcaccttat cgcccttctt caacgcctcc
    12421 aacatcgcct tctgttgttt ctgcttcttt tgttgaggca ggatcaacaa gaaataaaag
    12481 atgacgaaaa tcagcacaaa cggcacgagt gacagaagcg tccctgctcc cgatccgcct
    12541 ccgccggttc cctgagccca cgcgacagat tccatgaaca tacaacaggt cccctctctc
    12601 gattaaacgt ccagtgcagt tcgccgtatt gttccggcct cacgattcgc caatgcggca
    12661 acctccggcg cttcccgctc ttgggcgcga taaaacgcct ctcggaattc gctgaacgtg
    12721 ccctgcccga tcgccacccg catccggcgc ataaaatctg caaaatacca gaggttgtga
    12781 atcgtattca gccgcgaggc gagcatctct ttcatgtgat acaaatgatg gaggtaggct
    12841 cgggagtatc gcgcgcagac tgggcagccg caggtcggat cgatcggttg ttcatcgcgc
    12901 tgataccgtg cttgtttgat caggacacgc ccgaaactcg tgaacaacca gccggtcctg
    12961 ccgtgacggg acggcaccac gcagtcgaag agatcgatcc cgcgcgccac gccttcgatc
    13021 aggttctccg gcatccccac ccccatcagg taccgaggct tcgacacggg caactcaggc
    13081 acggtg
//
LOCUS       DATIFR010000126        10561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2382437, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000126 DATIFR010000000
VERSION     DATIFR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382437"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VLD60_07285"
     CDS             <1..270
                     /locus_tag="VLD60_07285"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HSB44796.1"
                     /translation="DLDHDGLHEMLMHDDGAEGDQIAGDGIYSAVWEQDGVLLAWTVS
                     PNRPGSLSDSGFVTVEARATYMTGSGPREVRVATIRANPVFAGSH"
     gene            284..1027
                     /locus_tag="VLD60_07290"
     CDS             284..1027
                     /locus_tag="VLD60_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44797.1"
                     /translation="MRMDGKTWEQITFLRRVESGTSLLELISAMAAGLLVLGATLQSL
                     SYFQQRFSHQQDRLAQQQDLRLGLELLEEEFRLAGVGSLSVILPDEIEFTANIHGLMT
                     NVTAAAAIGQTTLAVDDGRGWPDRKTVQVCWHEQCEQFTLARPGQRNLLTFMEPIPRM
                     IPIGAAVSVSNRVRYYSRPDERGVMRLLRQIDGGASVLVGDIEAVKFSYWDEQGRITA
                     QPLRVKRIIVELSLPRSASKAIREMSLRS"
     gene            1058..2536
                     /locus_tag="VLD60_07295"
     CDS             1058..2536
                     /locus_tag="VLD60_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44798.1"
                     /translation="MRQQQWRESTILGTQGHVLMASLMLVIILALLGMTALYLVSQDA
                     PGITAMREDNLAQQLSDAAAELVLSWFHDAGTTPPPIADLLAKRLGDVDTGPSFFDAA
                     GRSQFIGTADRPDILLDAADSGTDRFLNSPPSGFDGPLHALGRLEKLKVYGPSQPGLL
                     GTLEVTASTVGRRPLARTIRLQLGALNIPAIRAPVQTGQGLGAPQPGGASPVFAHWGD
                     VRVTGDLSVNRVEDVIVKSSSAPVTGQSYDLTGRLEDRWTDYWIGGNITVVAPPSSSG
                     SQVFPLNVHLQQQPVPGVRLDRWDYEGLKRTALRHGTYYRLDRTGRLHPLGATDSDPG
                     LLPSEVLAVPAVGRSQGLVFIDTVDGEAPRPDNLGTLVVDVDYVEALLVVQGHVLLRP
                     SGAGRSVPVLSPSPEGSGALGSRIPVTLSDIHFNGVLYAAGTITLERSIRLYGAVMTA
                     GTVSTSSPGLVMEVWYNADFGRGLFRGLPLVYRAQGTRQIVY"
     gene            2580..4391
                     /locus_tag="VLD60_07300"
     CDS             2580..4391
                     /locus_tag="VLD60_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSB44799.1"
                     /translation="MKGLSGEAASLEPSDKKTGRGNRFVRASIEQNVLDSLVYRGILS
                     QADLDHAVEDAKEGAQDLEALLLDRYHVQKEILGDALSSYYQCPYLPYDERTVIDTDL
                     LKTLNHDYLKKNLWLPIARHGQRLDVLTHDPHDLEKGWDVRRSFPGMSIRYTVGLRRD
                     IEQFLQAVTGPGASSSIGTILSDLVQDIPLEPTSEFQPSAVDENDSAIVRLANHLITE
                     ADRLGASDLHIEPFADKKETVIRFRIDGTCFTYMRVPAGYRRALVSRIKIMANLDIAE
                     RRKPQDGKIRFKLGAQREIELRVATLPTAGGNEDAVLRLLTAKEPMPLEAMEFAPEVL
                     HTIKMLAEKPHGIILCVGPTGSGKTTTLHAILRHINTDERKIWTAEDPIEITQDGLRQ
                     VQVHQKIGLSFAMAMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVLSTLHTNS
                     AVETVIRLLDLGCDPFNFADAMLGVLATRLCKRICAQCKEAYHPSRKEFDELVEGYGT
                     RFWKPLTMQYSEQLTLYRARGCEACRHTGFKGRVPLHELLLGTEDMKQLIQGKTRTAE
                     MLPKAIESGMVTLLQDGILKTLQGLTTYRNVRAVAIE"
     gene            4764..4892
                     /locus_tag="VLD60_07305"
     CDS             4764..4892
                     /locus_tag="VLD60_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44800.1"
                     /translation="MTRQNVHKGRPIDKGQFSNSKALVIGCYQRYPHEAWGKKVRD"
     gene            6333..6818
                     /locus_tag="VLD60_07310"
     CDS             6333..6818
                     /locus_tag="VLD60_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSB44801.1"
                     /translation="MQMFTARQTGFTLVELMIAVAIIGIAAALGMPDYLRWQAQSQLR
                     AATSEIATQMTLARMAGMNRNRSVNVTVQNLGGQIHISAVSSSGAVVIDDKSFTSGVM
                     SVVGSPITVSFSSLGIRTNGGTGVQSIGVCDRYNRQYSVTIIPSGKVNWSANSSGTPC
                     P"
     gene            6899..7345
                     /locus_tag="VLD60_07315"
     CDS             6899..7345
                     /locus_tag="VLD60_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44802.1"
                     /translation="MLAAVLLGTGLLAMSGMQAIALVKNVDASELTRITTLASDMMER
                     VYFNRRNAVSYNGIDTQSPSNCSTISVTAQPQAKGDCQLWDSLVKNTQLENVQGKVTV
                     DLNPIPPTALGQRNVTVTISWLGSVNSGSTKKRPRSLTLNRVVAPE"
     gene            7363..8460
                     /locus_tag="VLD60_07320"
     CDS             7363..8460
                     /locus_tag="VLD60_07320"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HSB44803.1"
                     /translation="MVMQTRRSLTRASAEQAGVTLIELMFGLAVGVIVVAAAFTILTT
                     TSKSLRANEQIVDTQQNVRLAMELLARDLKMAGFGNPGVQIGNCTNVIVPNDQTATGA
                     DSGPDSVQLLVPTTRTDATYTDPKRWTLYSPTTSAGIAQIKLQPGAVADMVSSGLVAN
                     QSYISIGGSSTVKVTGFDASASTIDVSIPPPLWFQQEDQVYLLQCIRYQVVLPPDPGN
                     LCMGNAPCLTRGVAGAAVTSPNAETPIAEGIEDLQLAYACDGCVAAINSGLPDRIIDD
                     VSGNGSFDLADFLSNVPWTTAPLTPDKIQLVQVALVARQSKTDQGFGETSGAVVGSPA
                     MTVTSDHTLPADPNHRRRILVKTVETRNVGL"
     gene            8525..9124
                     /locus_tag="VLD60_07325"
     CDS             8525..9124
                     /locus_tag="VLD60_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HSB44804.1"
                     /translation="MALLTVIMVLLMLTVLGIAAITASSLENNMAGMQRTMESAGQAA
                     ESCLGTGANVILQTLLPENGAAIPAVLVAPAGPVVATNNNPTILGNEIMGNPENNIDV
                     AVGVGPTGLAAAPNIQMTVGPYNVVGDIDRLYIKLRAGSGQQQFAAYDGTGVGAGSNG
                     SDVYFRITCIATNTATGTENRVSSLYACALTGDGCQKQP"
     gene            9244..>10561
                     /locus_tag="VLD60_07330"
     CDS             9244..>10561
                     /locus_tag="VLD60_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44805.1"
                     /translation="MKRKYIEAALIRLLATALLCASVGLGLPGKASAQNMADYTNYPI
                     FLNQNVPPNVLFLVDMGNYTLEAAYAGSNHWYPLSYKTGTATVGAGNIPYYAANVTVD
                     GATASGGGVDNTDLRAVDLNGNVLTGATESSPADTFNSSYAYYGAFDPLRCYSYSSNS
                     FVYGSVKATVSDTCATSMWDGNFLNWLTMRKQEVIYQVLVGGRPLPAQANLDGTANSL
                     AGTNLTGENGSTTGCTQSGGGSNITACWRYVKFVDNTTLSGRVPSGLPSPTVNLNSGG
                     TITTGRFFGSGEGTIYVNDNATVSPFDDSPGNQYNIKVDLTSEPDVPSGTGVNGTCPD
                     PNLSTFAGHRVCYMRERSLGVFQTMRLDNMHVAVMFVNASTGNGGSLQFAFEQNFIAS
                     SVTNIRNSQTQAYAPLAESLYEALCLYRKSQGPCYDNGTGTVWGTNY"
BASE COUNT         2342 a         2874 c         3151 g         2194 t
ORIGIN      
        1 gatctcgatc atgacgggct gcacgaaatg ctgatgcatg acgatggggc agagggtgat
       61 caaatcgcgg gggatggaat ctactcggct gtctgggaac aggatggagt cttgctcgcc
      121 tggaccgtta gccccaatcg ccccggcagt ctctcggatt ccgggtttgt caccgtcgaa
      181 gcgcgcgcca cgtacatgac aggcagtgga cctcgcgagg tccgagtcgc cacgatacgc
      241 gcaaacccgg tatttgcagg aagtcattag agcaggtgtt tcgatgcgga tggatggaaa
      301 aacatgggag cagatcacat ttctcaggcg cgtcgaatcg ggtacgagtt tgcttgaact
      361 gataagcgcg atggccgccg ggctcctggt gctcggcgcg acactccaat cgttgtcgta
      421 ttttcaacaa cggttctcac accaacaaga tcgactcgcg caacaacaag accttcgtct
      481 ggggctcgaa ttgcttgaag aggaatttcg gctggccggg gtggggtcgc tgtcagtgat
      541 cctgccggat gaaatcgaat ttacggcgaa cattcacgga ttgatgacca atgtgacagc
      601 cgccgcggcg atcggccaga cgaccctcgc cgtcgatgat ggtcgcggat ggccggatcg
      661 gaagaccgtt caggtctgtt ggcatgagca gtgcgaacag ttcacgttgg cgcgccctgg
      721 gcagcggaat ctcttaacgt tcatggagcc gatcccacgg atgattccta tcggcgcggc
      781 tgtctcggtg agcaaccgtg tgcgttatta cagtcgaccg gacgaaagag gagtgatgag
      841 actgttgcgc cagatcgatg gcggggccag cgtgttagtc ggagacatcg aagccgtgaa
      901 gttttcgtat tgggatgagc agggacggat cacggcacag cccctccgcg tgaaaagaat
      961 catcgtcgag ttatcgcttc cacgctccgc gtccaaggca atcagagaga tgagtctccg
     1021 gagctgaccg gagatctgat gcaatgaaga ggtgaccatg agacagcaac aatggagaga
     1081 atccacgatc ctcggaactc aggggcatgt ccttatggcc tccctcatgc tcgtcattat
     1141 ccttgcgctc ttgggcatga ccgcgctcta cctggtcagc caagacgcac cgggcatcac
     1201 ggcaatgaga gaggacaatc tcgcgcagca gttatccgac gccgcagccg aattggtgct
     1261 gagctggttc catgatgcag ggacgacgcc accaccgatc gccgacctgc tggcgaaacg
     1321 cctgggcgac gtcgataccg gtccctcttt cttcgatgca gccggccgct cccaatttat
     1381 aggaacggcc gatcggccag atattctgct cgatgcagct gattccggga ccgatcgttt
     1441 cctgaacagt ccgcccagtg gatttgacgg ccctttgcat gcacttgggc gcttggagaa
     1501 gttgaaagtc tacggtccct cacagccggg attgctcggc accttggaag tgacggcatc
     1561 aaccgtcggc cgaagacctc tggccaggac gatccgattg caactggggg cgttgaatat
     1621 tcctgccatt cgcgctccgg tgcagacggg acaggggttg ggagctcccc agccaggcgg
     1681 agcctcacca gtgttcgccc actggggtga tgtccgcgtg acgggcgatc tgtcggtcaa
     1741 tcgagtggag gatgtcatcg tcaagagcag cagcgcgcct gtgacgggac agtcctatga
     1801 cctcacgggg cgattggaag atcgctggac cgattattgg atcggcggca atatcactgt
     1861 tgtcgctcct ccatcatcga gtgggagcca ggtctttcct ttaaatgtac atctccaaca
     1921 acagccggtt cccggcgtgc ggctcgaccg gtgggactac gaaggattga agcgaacggc
     1981 gctacgccat ggcacgtact accggctcga tcgaacgggc cgactccatc cgctcggcgc
     2041 aacggattcc gatcccgggc tgttgccttc cgaggtgctg gcggtgccgg cagtcgggcg
     2101 gtctcaagga ctcgtattca tcgacaccgt ggacggagaa gcaccgcgtc ccgacaatct
     2161 tgggactctc gtcgtggatg tcgattatgt tgaagccttg ctggttgtac agggacatgt
     2221 gttgttgaga cctagcgggg ctggacgttc agtgccggtc ttgagtccat cgcctgaagg
     2281 atcgggcgcg ctcggcagcc gaattccagt cactctctca gacattcatt tcaacggggt
     2341 gttgtatgcc gctgggacga tcacgcttga acgttcgatt cgcctctacg gtgccgtcat
     2401 gaccgccggc acggttagta caagcagtcc ggggttagta atggaggtct ggtacaacgc
     2461 ggatttcgga agaggccttt tcaggggatt gccccttgtc taccgcgcgc agggaacccg
     2521 gcaaatcgta tattagcaaa caatgtgggg ataggcgcga tcgtcggggg cacgtgtcca
     2581 tgaaaggatt aagcggggaa gccgcctctc tcgaaccgtc ggacaagaag accggccgtg
     2641 ggaataggtt cgtgcgtgcg tccatcgaac aaaacgtgct ggattcgctg gtctaccgcg
     2701 ggatcctctc tcaggccgat ttggatcacg cggtcgaaga tgccaaagaa ggtgcacaag
     2761 acctcgaggc cctgctgctg gaccgctatc acgttcagaa ggagattctc ggcgatgccc
     2821 tgagcagcta ctatcaatgc ccctatcttc catacgacga gcggacagtc atcgataccg
     2881 atctattgaa aacgctgaac catgactatc tgaagaagaa tctctggctg ccgattgcac
     2941 ggcacggaca gcgcctcgat gtgttgacgc atgatccgca tgatcttgaa aagggatggg
     3001 acgtgcggcg cagctttccc ggtatgagca tccgctatac ggtcggtctt cgccgggata
     3061 tcgaacaatt tcttcaggcg gtgacggggc cgggagccag ttcttccatc ggaacgattc
     3121 tcagtgatct ggttcaggat attccgcttg aaccgacgtc cgaattccag cccagcgcag
     3181 tcgacgaaaa tgattccgcc attgtgcggc tggccaatca tctgatcacc gaagccgatc
     3241 gtcttggagc ctcagacctg cacatcgagc cctttgcgga caagaaagag accgtcattc
     3301 gatttcgcat cgacgggaca tgctttacgt acatgcgggt tcccgccggc tatcgtcggg
     3361 ccctcgtctc gcgcattaag attatggcga atctcgacat cgccgagcga cgcaagccgc
     3421 aagacggaaa aatccgattc aaacttgggg cgcaacgcga gattgaattg cgcgtggcaa
     3481 cgctacccac tgcaggagga aatgaagacg ccgtcttgcg cctgctaact gccaaagagc
     3541 cgatgcccct ggaggcaatg gaattcgctc cggaggtgtt acatacaatc aagatgcttg
     3601 cggagaaacc gcacggcatc attctctgcg tcggccccac cggatcagga aagacaacga
     3661 cgttgcacgc catcctacgg cacatcaata ccgacgagcg gaaaatctgg accgctgaag
     3721 accccatcga aatcacgcag gacggtttgc gccaagtgca ggttcatcag aaaattgggt
     3781 tgagcttcgc gatggcaatg cgtgcgtttc tgagggctga tccagacgtc attatgattg
     3841 gcgaaatgcg cgataaggag acggccgata tcgcgatcga ggcctcactc accggacatt
     3901 tggtgctcag cacgctccat accaatagcg cggtcgaaac agtcatccgt ctgctcgatc
     3961 tcggttgcga tccgttcaac ttcgctgatg cgatgcttgg cgtgctggca accaggctct
     4021 gcaagaggat ttgcgcacaa tgcaaagaag cctatcatcc ttcgcgcaag gagtttgatg
     4081 agttagtcga aggctacgga accagattct ggaagcctct caccatgcag tacagcgagc
     4141 aactgacgct ctatcgagcg cggggttgtg aagcctgccg tcacaccggc ttcaaggggc
     4201 gggtgccact tcatgagttg ctgctgggca ctgaagacat gaagcaactt attcagggga
     4261 aaactcgaac ggctgagatg ctgccaaagg ccatcgaaag tgggatggta acactcctgc
     4321 aagacgggat tctgaaaaca ctccaaggcc tcactaccta ccgcaacgtc cgggctgttg
     4381 caatcgagta actcgataga gtctccgaga cgatagcgcg tactttcagt ccctcttatc
     4441 gatgggaggt atagaacggc gactcaatcg cctcatggac tgctttgcct tgtaggctcc
     4501 tgctgtgaaa gtggcccgcg tgattgggat cgatcggcac agcgctgagg aggcgcgtac
     4561 attatccgga ggcatctgac gtgggaatgt gaacgccgtt cttgcccccg cagtgaagac
     4621 gaagcgacga gtcatagttg aggtcaccga agaagcttaa tgggcgaggt gctgcggcaa
     4681 agtagtcaat ttctggatcc tgactaggcg aggcaagggc tgcaccagcc agggacacaa
     4741 tgtgactcgg gatgcatggc agcgtgaccc gccagaatgt gcataaaggg agaccgatcg
     4801 acaaggggca attttccaat tccaaggcgt tggtcatcgg atgctatcaa cgttatcccc
     4861 atgaagcatg ggggaagaag gttcgcgact agccgaaggc agcctcgtaa caggatgaaa
     4921 gcttccaggg cgatgccagg agcagattga agtgcgatgg gcgcatacac cccgtgcaat
     4981 cagatctttc cgtatctgaa ggatgcggaa ttatgtttga gtcattggca tgatgatctg
     5041 ccgctactga tcaggcgaat cttaaaaaca gctgaacttg ttctgggctc gctgcatatt
     5101 ctacgtagtt gtttggttgt gtgcgatgct cgaatggcag taggctcatt ccaatgcgcg
     5161 ctgcacgaaa ttgctcccaa cgaatatttg tcacttgcgc acaaattgtc gtgtaggcaa
     5221 tgccctcgtg ggggaattgc cctttatttt cgcagagtct ggactcagat agcctggcac
     5281 ggcgcatgcg agattgatcg ggcaatgccc tgtgtgtatg gccggtcggc ccatagggtt
     5341 ctcgcaatca ggtaccaagg aggtacgtat gtttaagcgg actatggttg ccgcaagtgc
     5401 tgttctctcc ctccctctca ttttttcggg agtgggagtg ggcgtggcac agaatgtggt
     5461 ctgtgatccc cactgttccc ccacctatgg caactcaacg agtgatagtc accggggcca
     5521 ggggctgagg catgcgaatg acgttgccgg cgaccacgga ttcgagggcc gggacaacgc
     5581 tcggcggaaa caggatgctc acagacctgg agggtccgga gtgcccgatc cgacgccagc
     5641 accggtaccg gtaccgcctc caaccaccac tacgactaca gatccggcgc cggcccctgc
     5701 tccgactgtt tcgcctacct gttcactatg ctagcagcac aagtcgcata gacggagggc
     5761 ggccaggaga ctggccgccc tcgcgttctg tcgtctccca catctcgagt ccccagtcct
     5821 catgccgcaa ggcaccgtcc tggaatctca agaaagattt tacagatgaa cgatgtgccg
     5881 ggcggtctgg tgatcggaat accagagaag aagtcgcacg gtctttaaag aggcgaggct
     5941 tgttatttac cgtcgacgag cgatgctgtc tcaagggtac gctctggact ctggaggctc
     6001 cgcggagacg gaccaaaaat caggcaacgc agaatatcct cgagcatgaa ggcatctttt
     6061 cgtctccccg tgccctgcaa cgagttgggt cgatgcgtga ttgaggtgtg ccgatggcca
     6121 cacgttacca gaacacttct tgatgacggg cgacaaaaat tgtcgactcg ctactgtttt
     6181 caagtggccg acgaggagag aacgatcata ggaggcagga gattcaatag cttagcaaag
     6241 aatgagagaa ggcaggatcg gcacgcccct tgctctgctt gaatgtgcct cgttctgtct
     6301 actgacgggg ccagcttcag agggctagca cgatgcagat gttcacagcc agacaaaccg
     6361 gtttcacgct ggtcgagctg atgatcgcgg tcgccatcat aggaatcgcc gcagcccttg
     6421 ggatgcccga ttatttacgg tggcaggcgc agtcccagct tcgggcggcg acatcggaga
     6481 ttgcaacgca gatgaccctg gctagaatgg cgggaatgaa ccgaaacaga tctgtgaatg
     6541 tgaccgtgca gaatttgggg gggcagattc acatttccgc agtgtcctca tcgggagcgg
     6601 tggtgattga cgacaagagt ttcacctccg gggtgatgtc ggtcgtcggg agtccgatca
     6661 cggtgagttt ttcatcgttg ggcatccgaa cgaacggagg caccggggtg cagtcgatcg
     6721 gagtgtgtga caggtataac cgtcaatatt ccgtcacgat tattccatcc ggcaaagtga
     6781 actggtctgc gaattcaagc ggaactcctt gcccatgatg ttttcacggg tagacacgat
     6841 gcggagacac ggcttttccc gatgcaaggg ggaacaaggc ttttccttgt tggaaggaat
     6901 gctggccgcg gtgctgttgg gcacgggcct tcttgccatg tcgggaatgc aggcgatcgc
     6961 gctggtcaag aatgttgatg cgagcgaatt gacacgcatc acaacgttgg cgtcggacat
     7021 gatggagcgc gtctatttca accgacggaa cgcggtcagt tataacggga ttgatacaca
     7081 gagtccctcc aactgctcga ccattagtgt cacggcgcag ccgcaggcga agggagactg
     7141 tcagctctgg gacagtctcg tgaaaaacac ccagctcgaa aatgtccagg gcaaggtcac
     7201 ggtagacctg aacccgatcc cgccgacggc ccttggccag aggaatgtca ccgtgactat
     7261 tagctggctg ggctccgtga acagtggttc cacaaagaag cgaccgcgat ccttgacgct
     7321 caatcgagtg gtcgcgcccg aatgacggga gaagacacga ctatggttat gcagacccgt
     7381 cggtcgctca cgcgcgcctc cgctgaacaa gcgggagtca ctctgatcga actcatgttt
     7441 ggcctcgcgg tcggagtcat cgtcgtagcg gcggcgttta ccattctgac gaccacgagt
     7501 aagtcgttgc gggccaatga gcaaatcgtc gatacccagc agaatgtgcg gctggcgatg
     7561 gagttgcttg ccagggatct caagatggcc ggttttggaa atcccggtgt gcagataggg
     7621 aattgcacga atgtcatcgt gccaaacgat caaaccgcca ccggcgccga tagcggaccg
     7681 gattcggtgc aattgctggt accgacaacg aggaccgatg cgacttacac cgatccgaaa
     7741 cgatggacct tgtacagtcc gacgacctcg gcgggcatcg cacagatcaa gcttcagcca
     7801 ggagcagtgg ccgacatggt gagttcaggc cttgtagcca atcaatctta tatttccatt
     7861 ggggggtcct ccaccgtgaa ggtgacggga tttgacgcct cggccagcac cattgacgtg
     7921 tcgatccctc cccctctctg gttccagcag gaagaccagg tctacctgct gcaatgcatt
     7981 cggtatcaag tcgtgcttcc ccctgatccc ggcaatctct gcatgggcaa tgccccttgt
     8041 ctgacgagag gcgtagccgg ggcagccgtg acgagtccca acgctgagac cccgattgcg
     8101 gaagggattg aggatcttca actggcctat gcgtgcgatg gctgtgtggc agccatcaac
     8161 agcgggcttc ccgaccggat catcgatgat gtgagtggga acggcagttt tgaccttgcg
     8221 gattttctga gcaatgttcc ttggacgacg gcaccgctca ctccggacaa aatccaattg
     8281 gtgcaggtcg cgttggtagc cagacaatca aaaactgatc aagggtttgg ggagaccagc
     8341 ggagccgtcg tagggtcgcc agccatgacg gtgaccagtg accatacgct tccggccgat
     8401 ccgaatcatc ggcgccggat cttggtgaag acggtggaga cccgaaatgt cggtttgtaa
     8461 cggagcagct cgggtgagag atcaatgcag ctggtcgctc ggcagactga acgacgagcg
     8521 aggcatggcc ttgttgacgg tcataatggt gctgttgatg ctgacggtcc tcggcatcgc
     8581 cgccatcacg gccagcagct tggaaaacaa catggcggga atgcaacgga caatggaatc
     8641 ggcggggcaa gcggctgaat cctgcctggg gaccggagcc aatgtcattt tgcaaacgct
     8701 gcttccggaa aacggcgcgg cgatcccggc ggtccttgtg gcgccggccg gaccagtggt
     8761 cgccacgaac aataacccga cgattcttgg caacgagatt atgggaaacc cggaaaataa
     8821 catcgatgtg gcggtcgggg tcgggccgac cgggcttgcg gccgccccca acattcaaat
     8881 gaccgtgggg ccctataacg tcgtcggcga tatcgacagg ctgtatatca agctgcgagc
     8941 cgggtccggc caacagcaat ttgccgccta cgacgggacc ggtgtcggcg ccggctccaa
     9001 tggctccgac gtctatttcc gtatcacctg tatcgccacg aatacggcca ccgggacgga
     9061 aaatcgcgtg tcgtcgcttt acgcctgcgc cctcacgggc gacgggtgtc aaaaacagcc
     9121 atgaggaagg tgccgaaggc catgtcgcgg tttcgcatgg atggttcgtg caacgttgaa
     9181 aatctacact cccacgaccg ttcgcggcaa cgcgtcggtt gtgcgtttca ccgggaggac
     9241 aacatgaagc ggaaatacat cgaagcagcc ttgattcgtt tacttgcgac tgcactgctt
     9301 tgtgcgtccg ttggattggg acttcccggg aaagcgtccg cgcaaaatat ggctgactat
     9361 acgaactatc cgatctttct caatcaaaac gtcccaccga acgtgttgtt cctcgtcgat
     9421 atggggaact atacgctgga agccgcctat gccggatcaa accactggta tcccctctcg
     9481 tataaaaccg gcacggccac tgtcggtgcc ggtaacattc cgtattatgc tgccaacgtc
     9541 acggtggacg gcgccaccgc cagcggcggt ggcgtcgata acactgatct gcgggcggtg
     9601 gatttgaacg ggaatgtgct cacgggcgcg acagaatcgt ctcctgccga taccttcaat
     9661 tccagctatg catattatgg cgccttcgat ccgctccgct gctacagcta tagcagcaat
     9721 agcttcgtgt acggatcggt caaagcgacg gtgagcgaca cctgcgcgac cagcatgtgg
     9781 gatggtaatt tcctcaattg gctgacgatg cgcaagcagg aggtgattta ccaggtcttg
     9841 gtcggaggga ggccgcttcc cgcccaggcc aacctggatg ggacagcgaa ttctttagcc
     9901 ggaacaaacc tgaccggtga aaacggctcc acgaccgggt gcactcagtc cggcggcggc
     9961 agcaatatca cggcatgctg gcggtatgtg aaattcgtcg acaacaccac actctccggc
    10021 cgtgtgccaa gcggtttgcc gtcgcccacg gtcaatctca acagcggagg gaccatcaca
    10081 acaggccgct ttttcgggtc aggggaggga acgatctacg tcaacgacaa tgcaacggtc
    10141 tcgccgttcg atgacagccc cggcaatcaa tacaatatca aagtcgattt gacgtcggaa
    10201 ccggacgtgc cctcgggcac aggcgtcaac ggcacctgcc ccgaccccaa cctttcgacg
    10261 tttgccggcc accgggtgtg ttacatgcgt gagcggtcgt tgggagtctt tcagacgatg
    10321 cggttggaca acatgcatgt agcggtcatg ttcgtgaacg cctcgaccgg gaacggtggg
    10381 agcctccagt tcgctttcga gcagaatttc atcgcgtctt cggtcacgaa tattagaaac
    10441 agtcaaaccc aggcctatgc acccttggcg gagtcgctgt acgaagcctt gtgtctttac
    10501 cgcaaatcgc aaggcccctg ttacgacaac ggcaccggca cagtgtgggg cacgaattat
    10561 a
//
LOCUS       DATIFR010000127         1826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1042430, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000127 DATIFR010000000
VERSION     DATIFR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042430"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VLD60_07335"
     CDS             complement(<1..1431)
                     /locus_tag="VLD60_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSB44806.1"
                     /translation="MSLDLAKKLYTKMPEVFTKARKKFGRGLTLAEKVLVSHADNFDT
                     QTWERGKAMLALRPDRVAMQDATAQMAMLQFMQANKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVAYLNITD
                     RADLANLAISHKELLVADPEVYQSPEKYYDQIVEVDLSKLEPHVVGPHTPDLARPISK
                     LAAEAKEKGYPVELKAALIGSCTNSSYEDISRSAHIAQQGLKAGLKAKTSFLVSPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGINETLSFLASPEVVTAYAITGDLG"
     gene            complement(1489..>1826)
                     /locus_tag="VLD60_07340"
     CDS             complement(1489..>1826)
                     /locus_tag="VLD60_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSB44807.1"
                     /translation="RNPDKRVKELVGYVKSLNIKTPCLDFALAVEQVTAAKKDNLILN
                     VDGTMAAVLVDIGFPVDSLNGFFILSRTIGLIGHWVDQKRQDSRLIRLFDYLVNYAAP
                     KRREVPPLK"
BASE COUNT          380 a          576 c          465 g          405 t
ORIGIN      
        1 gcccaaatcc cccgtgatgg cataggccgt cacgacttcc gggcttgcaa ggaacgacaa
       61 ggtttcgttg atgccatcat tgcggcctgg gaagttccga ttgaacgaac tgacgatcga
      121 atccgccttg cccttcaccc catcggcccg cttccactga ccgatgcagg ggccgcagga
      181 gtttgacaac accgttccac ccaacttctc gaacgtgtcc aggaatccat cccgtttcat
      241 cgtatgataa atgcgctcgg agccaggcga cacgaggaag gacgtcttcg ccttgagccc
      301 cgccttcaaa ccttgctgcg caatgtgagc cgaccggctg atgtcttcat acgacgaatt
      361 ggtgcagctg ccgatgagcg ccgccttcaa ttcgaccgga tagccctttt ccttcgcctc
      421 cgccgccaac ttggaaatcg gcctggctaa gtccggcgta tgcggaccaa ccacgtgcgg
      481 ctccagcttc gagagatcga cttcgacaat ttgatcgtag tacttctccg gcgattggta
      541 gacttccgga tccgccacga gcaattcctt gtgagagatg gcgagattcg cgagatcggc
      601 ccggtccgta atattcagat aggccaccat cttttgatcg aaggggaaca cagaggtcgt
      661 ggcacccagc tccgcaccca tattacaaat ggtgccctta ccggtggcac tgatggtttc
      721 ggcgcccgga ccgaagtact cgacgatctt attggttccg cctttgaccg tgagcaaacc
      781 gcagagatac agaattacgt ccttcggcga ggcccagccg ctcaacttac cggtcagccg
      841 cacaccgatc aatttcggat ggagcacttc ccagggcagg ccggccatga cttcacccgc
      901 atccgctccg ccgacgccga tcgccaacat gccaagcccg ccgccgttcg gcgtatggga
      961 gtcggtgccg atgatcaggc cgcccggaaa cgcataattt tccaacacga cttgatggat
     1021 gatcccggcg cccggcttcc agaatccaat cccgtacttc ttcgcggccg aggccaggaa
     1081 gttataaacc tctttgtttt catccatcgc gcgaagcagg tccttctcgg aacccatctc
     1141 tgctctaata agatggtcgc agtgaatcgt gctcggcaca gcggcttttt tcttattggc
     1201 ctgcatgaac tgcaacatgg ccatctgagc cgtcgcatcc tgcatcgcca ctcgatccgg
     1261 acgcagggcc agcatcgcct tgccgcgctc ccaggtctgc gtgtcgaaat tgtccgcgtg
     1321 agaaacgagc accttctccg ccagcgtcaa cccgcggccg aatttcttcc gagccttggt
     1381 aaagacttcc ggcattttcg tataaagttt cttcgctaga tcgagtgaca tattttctcc
     1441 gtccggtttg gctgacggct gaaagccatt agctgtcagc attcagctct atttcaacgg
     1501 cggcacttcc cgtcgtttgg gcgccgcata gttcaccagg taatcgaaca gccggatcaa
     1561 gcggctgtcc tggcgcttct gatccaccca atggccgatc aagccgatcg tgcgcgagag
     1621 gataaagaag ccgttcaagc tatcgaccgg gaaaccgata tcgacgagca ccgccgccat
     1681 cgtcccgtcc acgttcagaa tcaaattatc cttcttcgcc gcggtcacct gttcaactgc
     1741 caacgcaaag tcgagacagg gtgtcttgat gttcaagctc ttgacgtatc cgaccaactc
     1801 cttcacgcgc ttgtccgggt tgcgca
//
LOCUS       DATIFR010000128         1303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1809305, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000128 DATIFR010000000
VERSION     DATIFR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809305"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>1303
                     /locus_tag="VLD60_07345"
     CDS             271..>1303
                     /locus_tag="VLD60_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44808.1"
                     /translation="MRISAPKDLSECRRFFLEPRNPKQRIYEALRAYFVDNRPPKEVA
                     RDFGYSVGSYHVLCHHFRRDPDPVFFVSPHHGPQSQPKKSAARDLIISLRKQNCSVYE
                     ISQTLKERGRPLSPTAVREVLKAEGFAPLPRRLDEERPDYPRPTVEAVADVREFSLEP
                     RAFSTRCGGLFLFVPDLVRLNTDRLAAAGRLPGSNMIPAEHALRASLALKLWSIERKS
                     HIMALVSDEGLALFSGLNAIPKKSYLSEYSSRITPIRTNRLLAEWHELVSDSDLFPGE
                     SFNLDFHSVPYYGEKPTIERHYVSARSRRQPSILVFLAQDAEGRAFCYSNADLLKGDE
                     AEEIFRFISF"
BASE COUNT          258 a          400 c          334 g          311 t
ORIGIN      
        1 tgctttgtcg ccacgacggc atttgaaatt ccggccattt ccatgggtac ccggctattc
       61 ggcttgtgcc gtaagtgtcg tcacttatca atatggtgtt ccgaatcatg atcgtgatac
      121 gttatagctc aacttttctc gaccttctcc aaatctctgc ggttctggaa tacgcctgtc
      181 atcgcttgta gaatcagcct ctcgggcaaa caatggattg tggctctgaa tctccttgac
      241 actacatatt gtgtgtttta tcgttggctc atgagaatca gcgcacctaa agacctaagt
      301 gaatgccgcc gcttcttcct cgaaccccgg aacccaaaac agcgcattta cgaggccctg
      361 cgggcctact ttgtggacaa ccggcccccc aaagaggtcg cccgcgattt cggctacagc
      421 gtgggctcct atcatgtcct ttgccatcac tttcgccggg atccagaccc tgtcttcttc
      481 gtttctcccc atcacgggcc tcagtcccag cccaagaagt ccgcggcccg cgatctgatc
      541 atcagcctgc ggaagcagaa ctgttcggtc tacgaaatca gccagacgtt gaaggagcgg
      601 ggccgaccct tgagtcccac ggcggtacgc gaggtattga aagccgaagg attcgctccg
      661 cttccacggc gtctcgacga agagcgtccg gattatcccc gtcccacagt ggaagccgtg
      721 gcagacgttc gggaattttc tcttgagccc cgggcgttca gcacgcgttg tggcggcctt
      781 tttctctttg tgccggactt ggtccggctc aacacggaca ggttggcagc cgccggccga
      841 ctgccgggtt cgaacatgat tccggccgag catgcgctgc gtgcatcgct cgctctgaaa
      901 ctctggtcga tcgaacgcaa gagccatatc atggccttgg tgtcggacga ggggctcgcc
      961 ttgttttccg gactcaacgc cattcccaaa aagagctacc tgtccgaata ctcgtcgaga
     1021 atcactccca tcaggacgaa tcgccttctg gccgaatggc acgaactggt atcggattca
     1081 gacctctttc cgggcgaatc gttcaatctc gactttcact cagtcccgta ctatggcgaa
     1141 aaaccgacga tcgagcgcca ctatgtctcc gcccgcagcc gtcggcaacc cagcattctc
     1201 gtcttcctgg cgcaagatgc cgaggggcgg gccttctgct attccaacgc cgacctgctc
     1261 aaaggggatg aggcggaaga gatctttcga ttcatctcct tct
//
LOCUS       DATIFR010000129        21730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2003232, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000129 DATIFR010000000
VERSION     DATIFR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21730
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003232"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VLD60_07350"
     CDS             <1..228
                     /locus_tag="VLD60_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44809.1"
                     /translation="LKTFTTDLTVTKRLAPRIPSDKLIVSESGIHERGHVEQLLEIGV
                     HAMLIGESLIRAENLGAKVRELRGVAASSPA"
     gene            225..872
                     /locus_tag="VLD60_07355"
     CDS             225..872
                     /locus_tag="VLD60_07355"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSB44810.1"
                     /translation="MSMLNRCKVKICGITNLEDAAVAVEAGADALGFICYRKSPRYVE
                     AAVVKRIVASLPPLVMPVGVFVNEDSATVRTVMDNCGLALAQLHGDETAAYCQELGRP
                     VMKALRLKDRASFLALAEFQGRAGVRGFVLDAFSEKAYGGTGHVIDWGLAAEAAKAAT
                     ILLAGGLTPENVAEAIRAVRPYGVDVSSGVESAPGKKDHGKVRALLQAVRVVSST"
     gene            915..2108
                     /gene="trpB"
                     /locus_tag="VLD60_07360"
     CDS             915..2108
                     /gene="trpB"
                     /locus_tag="VLD60_07360"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSB44811.1"
                     /translation="MAIVPDNRGRFGAYGGRYVPETLMPALLELEQEYAKAKKDRRFQ
                     AELAYYLKHYVGRPTQLYFAERLTKRLGGAKIYLKREDLCHTGAHKINNAIGQGLLAK
                     RMKKPRIIAETGAGQHGVATATVAAMFGLQCEIYMGTEDMQRQALNVFRMRLLGSTVT
                     GVDAGSRTLKDAISEAMRDWTTNIRTTHYILGSVLGAHPYPMMIRDFQSIIGKETRKQ
                     ILAAEGRLPACLVACVGGGSNAMGLFHAFIGDKKVKMIGVEAGGLGVASGKHAARFSG
                     GKPGVLQGTMTYLLQDEDGQINLTHSVSAGLDYAAVGPEHSYLREIGRATYTSATDDE
                     AMAAFDLLAREEGIVPALESAHAIAHTVKLAPTMKKQQILIINLSGRGDKDVQQVARM
                     RGITL"
     gene            2105..2920
                     /gene="trpA"
                     /locus_tag="VLD60_07365"
     CDS             2105..2920
                     /gene="trpA"
                     /locus_tag="VLD60_07365"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSB44812.1"
                     /translation="MTSRIDRTFARLHERKEAALIAYIMAGDPTLAETEQLVLELEGA
                     GADIIELGVPFSDPIADGPVIQQAAERALRSGTSLRKILPMVKQLRTKTQVPLVLMAY
                     YNNIHAFGPERFCREAVAAGVDGLIVPDMPPDEAGPLKGPAAESGLQLIFLLAPTSTA
                     ERRMFVARQSQGFVYYVSLTGITGAKLNNVAEVGRNVEKIRKVSKTPVAVGFGVATPD
                     DAANVSAVADGVIVGSAIVKRIAAHQQQPGMVTQVAGFVRSLKAAMASSPASV"
     gene            complement(3015..3623)
                     /locus_tag="VLD60_07370"
     CDS             complement(3015..3623)
                     /locus_tag="VLD60_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44813.1"
                     /translation="MFKLHQLRRSIFICLLSLLIAGCGGLQEVWEGPSAKVFRPQAIA
                     VLPPMASQYDSAREDIQDVLSAALTKVAHVERVISPETVTDVFQASKEAFDSLVFYFS
                     RLEMTGHSDKEAAIKLGKALNVDSFIVVRVNAWEYARKEGDNVGRVGLSLRLIDATTG
                     TTVWKARHEKSSSYMFYRPNLKDVATELANEMIAFMPPQPKR"
     gene            complement(3640..4767)
                     /gene="traF"
                     /locus_tag="VLD60_07375"
     CDS             complement(3640..4767)
                     /gene="traF"
                     /locus_tag="VLD60_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HSB44814.1"
                     /translation="MTRTSGPIRILLYLCLSLVATPAMAVEFAVVGPRAAGMGGAGVA
                     VTTDAYATYWNPAGLAMVKSMDIRIQGTAQGTARLGIASALHDLQDFNPNDTSAGNQA
                     RAQGIANQINQPGATISGVGTGGLYVKGHLGEHAFGFNVSDVFTSGGFVSSPVVLTGT
                     NIRGQMTLQGLEARQAAFSYAYAFADKTFALGVTAKLIQGAAYTSTANLEGGQDPNLL
                     DNLGKAKISTSYGIDVGAIYRPSSWLRFGVIGKDLNQPTFDAPNGSEFKLTPQVRGGV
                     AVNPWSTMTITADMDITSNKTLTPGLKSQVLSLGMEQTILSEFLSFRVGTYKNVQDAG
                     SIFTPTGGLGIRIYSFRLDIAGGYDFNEGGGIGSAALAWTF"
     gene            complement(4890..5552)
                     /locus_tag="VLD60_07380"
     CDS             complement(4890..5552)
                     /locus_tag="VLD60_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44815.1"
                     /translation="MITVTDLHKSFVIGEHHLEVLKGINLTIARGELIAIVGASGAGK
                     STMLHILGLLDQPTKGTVLYEGRDLFQLSETDQADFRNRRIGFVFQFHHLLPEFTALE
                     NACMPALIQRREPAEIEKEAVAILEEVGLGARLHHKPGELSGGEQQRVAVARALLQKP
                     DLVLADEPTGNLDTHTGDALFSLMRELNKTRGTTFVIVTHNDKLSAQADRIVHMQDGQ
                     II"
     gene            complement(5631..6908)
                     /locus_tag="VLD60_07385"
     CDS             complement(5631..6908)
                     /locus_tag="VLD60_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HSB44816.1"
                     /translation="MTLPYEIFVGLRYLRAKRRNRTISLNTFVSVAGITLGVAALIGT
                     VGIMTGFKEDIQAKILGTTAHIVVQDRMRDGMSNYNATTQQVEAVPDVVAATPFILKQ
                     VMLTTQTSAQGVVIRGIDPQREGKVTELAKNLATGQLADLSKPIQVSLPPPDNPTGPP
                     QPSEKPGIILGKELALRLGTFVGDTITVVSPAGPISAMGMVPKIRIFAVAGIFQSGMY
                     EYDSSLAYIDLAEAQKFFNMGDSVTGIEVKVTDVFRAGEIARRVEQALGFSYGARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATKK
                     SIRRIFMLNGLIIGLSGTAIGIPLGYTFLWLIETFWTFDPTVYYISKIPVHVLAEDVL
                     LVAGSAILISFAATVYPSFQAAKLEPVAALRYE"
     gene            complement(6915..8396)
                     /gene="lysS"
                     /locus_tag="VLD60_07390"
     CDS             complement(6915..8396)
                     /gene="lysS"
                     /locus_tag="VLD60_07390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSB44817.1"
                     /translation="MEELNEQRQQRIKKLDHLREAGVAPYGTRFEVKDRAGQLIKLHG
                     EKRKETLEQEKIPCTIAGRVVALRRFGKAAFAVLQDGSDRLQTYLKKDLLSEQAYAVA
                     EQLDLGDWIGVSGTLFRTKTNEFTVEVHQLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPEVHGIFATRSRIISGIRSFLIERGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LGVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADY
                     HDLIILTEELITSLAQQILGKTLIEYQGKEINLTPPWRRWSYHQAILEVNKLDESVLH
                     DKEKATAAAKQLNVPVDPKWPLFNIVNEIFEETVEPNLFQPTFITDYPIEISPLARRK
                     DSNPALTDRFELYIAGREIANAFSELNDPLDQRERFEGQAVQRAAGDEEAHLVDEDFL
                     RALEYGMPPTAGEGIGIDRLIMLFTNQASIRDVVLFPQLRPEK"
     gene            complement(8444..9587)
                     /gene="prfB"
                     /locus_tag="VLD60_07395"
     CDS             complement(join(8444..9517,9519..9587))
                     /gene="prfB"
                     /locus_tag="VLD60_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSB44818.1"
                     /translation="MLDEIRIRVRSVGEQVAELRGHLDLARMTAELEELEAKMGQPDF
                     WNDARAAAGVSRKKVKLERELHQWRDIESKLSDVTTLLELAQESGDAGLERELAAELD
                     QFEPLLAALRVEHLLSGELDQNNAIVAIHPGAGGTESQDWAQMLLRMYVRWAESKKFK
                     VDTLDLQGGDEAGIKSATISVTGPYAYGYLKAEAGVHRLVRISPFDSNKRRHTSFASV
                     FVYPELNEDIDVAVEDKDLRIDTFRAGGAGGQNVNKVETAIRITHMPTGIVVQCQNER
                     SQLQNRNGAMKILKARLFELEQKKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGYEVGQVAAVMDGDVDGFIEAYLKKKLTKGSDQLSAVSEPDDL"
     gene            complement(9605..11221)
                     /gene="lnt"
                     /locus_tag="VLD60_07400"
     CDS             complement(9605..11221)
                     /gene="lnt"
                     /locus_tag="VLD60_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSB44819.1"
                     /translation="MLQWPVRQIVLAVVSGVLLPFCFPKFDMGLLAWVALIPLHIALD
                     GTSKRQAFWLGWLSGVIGFTGIMAWVVTAMHTYGKVPLIISYGILLLLTAYLGLFVAI
                     YGAGMVWFRSLIPRYGLFAAPCLWVTLELLRTYLLSGLPWSLLGYSQYRQLDLIQIAD
                     HLGVYGVSFLIVLTNVALAELYLWLMPLFRKFRPARLPWELVSTSAILVGLSWAYSTS
                     LIVHEGFGPPKTTLQIGVVQPNIDQAVKWDQGFREETLRRYEQLTGTFGYGADLVIWP
                     EAATPFVYEREPVYQLQLVALANRASAPLLFGSPAVRYHADRTPYLLNSAYLLSPDGH
                     LLGRYDKQHLVPFGEYIPLKSSVLFFLDKLVEGIGDFQAGPGPTILSFQPKASSTGSD
                     PRPVKFSVAICYEVIFPDLVRRMAASGAEFLVTITNDAWFGDSSAPSQHFSMVVFRSV
                     ENHLAFARAANTGVSGFIDPFGRIIEATSIFTQAALQATIPVRQPATFYTRYGDVFAY
                     GCVIICLLLTLFGLFGTKGPEPDAIAATPV"
     gene            complement(11314..11655)
                     /locus_tag="VLD60_07405"
     CDS             complement(11314..11655)
                     /locus_tag="VLD60_07405"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB44820.1"
                     /translation="MTYQPDKRRSSRMPVACAVYYSDGAFHASGVTENLTTSGGCLHG
                     THSVHVGMELMLLLIPADQRALLIKKATVRWANEHRFGVELDEADCGAVGELEEEDFE
                     RQQGPLSILTH"
     gene            complement(11766..12023)
                     /locus_tag="VLD60_07410"
     CDS             complement(11766..12023)
                     /locus_tag="VLD60_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44821.1"
                     /translation="MISPGVGFFRGLIVMLPLTVTAMSYAPAHGLLTWLLTVGLLQRG
                     WPQHYALPTGMSAAFAFGLVMEICQAFVPDVSSIADMSFPT"
     gene            12539..13201
                     /locus_tag="VLD60_07415"
     CDS             12539..13201
                     /locus_tag="VLD60_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44822.1"
                     /translation="MNAQTVIGITCIAIMSSSCVMKSTYDASVRETDSLKAELERAKE
                     EQRLYAKQVKELEQSNNDTMQDAEATLTATMRAQTDVEAERRLAEEQEAKLKQKIAQL
                     IRQHHAMRGEIAVARENGAALQELVEVYKKKIAELPRDNVVSSSPNMTPTSKPFDPAL
                     LPPVQDLPPPAPAVEPSKPAPAPAPSTSPVPAKHKQEPAESGWLTTIKEWVVSLWRSI
                     FS"
     gene            13363..13761
                     /locus_tag="VLD60_07420"
     CDS             13363..13761
                     /locus_tag="VLD60_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44823.1"
                     /translation="MKRAWIAAGLCLSIAAAGCGGGGTAEVREDRLTVGKVQGEIKVG
                     LAAAQVAELLGSPNIVTTDEKRREVWIYDKVSTDRVDMAHSSFAGIILVMGGTSSESS
                     SSTRQRTLTIIIKYDEEKKVRDFAYNYTQF"
     gene            13781..14374
                     /locus_tag="VLD60_07425"
     CDS             13781..14374
                     /locus_tag="VLD60_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44824.1"
                     /translation="MSRWLIAAICLLFSSQLAGCVTQSQPEELFQLSPESPKHRAMQT
                     RMFETSDEKELLSASAATLQDLGFEIEESVSDVGFLRAAKERSAREWGQHINRFMMAI
                     VTTPLAIMEAGPVILPIDLHQRIAASLITRPLNPERSRYEVRIMFYRVVWKGEGAAGR
                     SSFPPGEQRSEMIRDPVLYQQFFARLSKAVFLEAHKI"
     gene            14371..14820
                     /locus_tag="VLD60_07430"
     CDS             14371..14820
                     /locus_tag="VLD60_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44825.1"
                     /translation="MMRPYQNARRTLGAWLVLAASLQLYGCAPEPSQDLFAPTEAQMK
                     LRSFQTRTFDVSDRQVALRAVIAVLQDLGFIIERANEPLGLVTAARFSEPNYLEIVGV
                     TVTVRPQGEGRMMIRANAIHNNKPIEDPKVYQNFFASLERSLFITKE"
     gene            14826..15440
                     /locus_tag="VLD60_07435"
     CDS             14826..15440
                     /locus_tag="VLD60_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44826.1"
                     /translation="MPMRARRDTTRPTISTPAVRHLAMGSVVALSLFAQGCLSYEWAQ
                     QIQSDSHDQVLASEASQVKLRAAQTRSFDTTDRTQVLRAAVFALQDLDFQIEVLDEDL
                     GILSGKKFVAIERPNLGYDPSYHLYDDTGLLLFNRTYFGWGPFWHRSDLVRMTVTVRK
                     RNEAQLIVRASAQFYLRPVEEPGPYQMFFRTLEQSLFLEGQVVE"
     gene            complement(15447..15704)
                     /locus_tag="VLD60_07440"
     CDS             complement(15447..15704)
                     /locus_tag="VLD60_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44827.1"
                     /translation="MATKNLDSRNRVRRRPVGGKKNRAAAVRADAGLNRRFVELREQI
                     DLVLPEMTARVAALEHLLLELKLCTRDDLRRAREFVRMQEA"
     gene            16722..17138
                     /locus_tag="VLD60_07445"
     CDS             16722..17138
                     /locus_tag="VLD60_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44828.1"
                     /translation="MKKPKTNIWTLLLAVAFALVITVLDLLPWFKGVPWNVCFVVPVV
                     WVALWSAEDDVLPLITVGLIVTILTMVRGVVFGGILDPTPITDRLIVGTIIWLTVLLA
                     VLRKRARRTFNWITLAGRQWEPDKPDNEKNQSDQTR"
     gene            complement(17158..18138)
                     /locus_tag="VLD60_07450"
     CDS             complement(17158..18138)
                     /locus_tag="VLD60_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HSB44829.1"
                     /translation="MLVTPEANRQNRRQAIVGAVLVAVVAGFLGWHSPALWLLLALSP
                     FTYWWVRRRCLRRMAVMRQPFPDEREQILRTHVAFFAALDDEGKTRFRQLVQIFLDEV
                     RITGIRTEVDETIRVLVAASAAIPIFGFHDWEYHRLREVLIYPNAFDDAYQSRGGADE
                     TILGMVGLHHLSGVMILSKPALLAGFNHQAGPHNVGVHEFAHLVEKEENEYGLPPEVP
                     WMAVRQWVRYVARELAHPSRRGARVGAYAYTSEHEFFAVLAEYFFTSPESLKRRDPAL
                     YAIMRDMFHQDTGSILPLVPGRRQGIGRNAPCPCGSGNKYKHCCLAKARA"
     gene            complement(18364..18549)
                     /locus_tag="VLD60_07455"
     CDS             complement(18364..18549)
                     /locus_tag="VLD60_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44830.1"
                     /translation="MFHLPLTNPAIATGCVRDITTGWHERQADGRDEREDVRGKAEGG
                     TSGTSGKGETGGTGLFR"
     gene            18541..20685
                     /locus_tag="VLD60_07460"
     CDS             18541..20685
                     /locus_tag="VLD60_07460"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44831.1"
                     /translation="MKHKPSARDLLPFGIIAAALLSGLIVQSLWSNRVWVNVPLHSTV
                     EALGGLAAVLMGLVLLARKAEWEDERLQGVASGLLGMGILEGFHAVSTPEHGFVLLRS
                     AASLLGGVAFSFVWHPRGMIGKRTRELIPYLVTAATVAFGILVLAFPHHLPRFTVEDQ
                     FAPIALAVNGVAAVLFLTGAAGFWLESGRTGRPEHQLLAALAILFGLAETMFLFSTPW
                     QSEWWVWHLVRLAAYVLALTYVSRGYVRMVGETRIALAEAKRSGRRLASQYAVTRVLA
                     ESASLKEAAQAILRALAENLEWELGMFWSRDEQMQCLRFVDLWHAPHVKAIEFVNDSR
                     GRTFQRGEGLIGRVWATGKPIWIPDVVTDPDFRRAPMAARAGLHGGFAFPVRKGEHTY
                     GVIEFFSRESREPDQDVLEMVADIGIKVGLFVDRKWTEEELRRTEARLVEEQRLAEVS
                     RVLGDIGHDLKNMLMPIVTGAGLLEEELSECFAKLPQPAMSAMKPSRDLTHDLIEMIR
                     QGGRRIHDRVKEIADSVKGLTRPPQFAPCRVADIVASVYATLNILAKEGRVALRTEGL
                     ETLPVIRADENRLFNAIYNLVNNAIPEVPSGGSVTVQGRADQSGKNIMLSVIDTGKGM
                     SPEVRDSLFSYQAISRKMGGTGLGTKIVKDVVDAHGGSITVESTPGVGTAFHITLSVD
                     GPPARTPNASPSPVETGRMTFGSASKEEHLGR"
     gene            21039..21638
                     /locus_tag="VLD60_07465"
     CDS             21039..21638
                     /locus_tag="VLD60_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB44832.1"
                     /translation="MNRHVQTVCLLAIGCMALWTAGCSSKGVKSDGSTGYPAPSQSEP
                     VPNLSMSDPPGTERLNSGIAVAKADPGAARRQMDEMRAEQSATAASGLRDVFFAYDSW
                     SINEEGRQALSRDAEWLRTHPTVQLKVEGHCDERGSSTYNLVLAEKRAKSVRNYLTEL
                     GIRKDRFVVVSYGKERPFCKERSEACYQQNRRGHLVVKP"
BASE COUNT         4586 a         6479 c         6312 g         4353 t
ORIGIN      
        1 ctcaagacat tcacgacgga tctgaccgtc accaaacgcc tggctccccg tattccgtcg
       61 gacaaattga tcgtgagtga aagcgggatc cacgagcggg ggcatgttga acagctcctg
      121 gagatcggcg tgcacgccat gttgatcggc gagtctctga tcagggcgga gaatctcggc
      181 gccaaggtcc gtgaattacg aggggtcgca gcctcgtcac cggcatgagc atgttgaatc
      241 gttgcaaagt caaaatctgc gggatcacca atttagagga cgccgccgtc gccgttgaag
      301 ccggcgccga tgctctgggg tttatctgct atcgaaagag tccgcggtac gtcgaggcgg
      361 cggtggtgaa gcggatcgtg gcgagcctgc cgccgttggt catgccggtc ggtgtattcg
      421 tcaacgagga ttcagccacg gttcgaaccg tcatggataa ctgcggtctc gcgttggctc
      481 aactgcacgg cgacgaaaca gccgcctatt gccaggaact tggccggccg gtcatgaaag
      541 cccttcgatt gaaagatcga gcgagtttcc tcgccttggc cgaattccag ggccgggctg
      601 gggtccgcgg cttcgtcctc gatgcctttt ccgagaaggc ctatggggga accggtcatg
      661 tcatcgactg gggattggcc gccgaagcgg cgaaggctgc cacgatactc ctggccgggg
      721 gtctgacgcc ggagaacgtg gccgaggcga tccgggcggt acgcccctac ggcgtcgatg
      781 tcagcagcgg tgtcgaatcc gcaccgggta agaaggatca cggcaaggtg cgggcattgt
      841 tgcaagcagt cagggttgtc tcctcgacct gaggccgttt atactgccga catttcaatc
      901 acagaaggat cagcgtggca atcgttcctg acaatcgcgg ccggttcggg gcatacggtg
      961 gacgctacgt cccggaaacg ttgatgccgg ccttgctgga attggagcag gaatacgcga
     1021 aggccaagaa agatcgacgg ttccaggccg aattggccta ttacctcaag cactatgtcg
     1081 gccggcccac tcagctctac tttgccgaac gtctgacgaa gcgtctcggc ggagccaaaa
     1141 tttatctcaa gcgcgaagac ctctgtcaca ccggcgctca taagatcaac aacgccatcg
     1201 ggcagggact cctggccaag cggatgaaga agccgcggat catcgccgaa accggcgcgg
     1261 gccagcacgg cgtcgcgacg gccaccgtcg cggccatgtt cggcttgcaa tgtgaaattt
     1321 acatgggcac cgaggacatg cagcgccagg cgctcaacgt cttccgcatg cgcctcctcg
     1381 gctcgaccgt taccggtgtg gatgccggaa gccggacgct caaagacgct atcagcgaag
     1441 cgatgcgcga ttggacgacg aacatccgca ccacccacta tattctgggg tcggtgctcg
     1501 gcgcgcatcc ctatccgatg atgatccgcg attttcagtc gattatcggc aaggaaacac
     1561 gcaagcagat tttggccgcc gagggccgat tgccggcttg tctcgtggcc tgcgtgggcg
     1621 ggggaagcaa cgcgatgggc ctgtttcatg cgttcatcgg cgataagaaa gtgaagatga
     1681 tcggcgttga agcaggcggc ctcggcgtag ccagcgggaa gcatgccgcg cgattttcgg
     1741 gaggcaagcc cggcgtcctg cagggaacga tgacttatct gctgcaagac gaagacggcc
     1801 agatcaatct gactcattcc gtgtccgccg gattggacta tgccgccgtc ggccctgaac
     1861 atagctattt gcgtgaaatc ggcagagcca cctacacctc ggccaccgac gatgaagcga
     1921 tggccgcctt cgaccttctg gcaagggaag aggggatcgt gccggcactc gaaagcgccc
     1981 atgcgatcgc ccacacggtc aaactcgcgc cgacgatgaa gaaacagcag atcctgatca
     2041 tcaacctttc cggccgtggc gataaagacg tgcagcaggt cgcacgcatg agagggatca
     2101 cgctgtgaca tcccgtatcg accggacctt tgcgcgcttg cacgaacgga aagaggcagc
     2161 gctgatcgcc tatatcatgg cgggcgatcc cacgctggcg gaaactgaac agctcgtcct
     2221 tgaattggaa ggggcaggcg cggacatcat cgagctaggc gtaccctttt ctgatccgat
     2281 cgccgacgga ccggtcattc agcaggcagc cgaacgagcg ctccggagcg gaacgtccct
     2341 tcgcaagatc ctcccgatgg tgaagcagtt gaggaccaag acgcaggtcc cgctcgtcct
     2401 gatggcctac tacaacaaca tccatgcgtt cgggccggag cggttttgcc gggaagcggt
     2461 ggcagccggc gtcgatggat tgatcgtgcc agacatgccg ccggatgaag cgggtcccct
     2521 caaaggaccg gcggcggaat ctggacttca gctgattttc ctgctcgcgc caaccagcac
     2581 ggccgagcga cggatgttcg tcgcccgcca gtcgcaaggg ttcgtctact atgtctcact
     2641 gaccgggatt accggcgcga agctcaacaa cgtcgccgaa gtcggacgga acgtggagaa
     2701 gatccggaaa gtctcgaaga ctcccgttgc agtgggattc ggcgttgcga ctcctgacga
     2761 tgccgcgaac gtgtcggccg tggccgatgg tgtcatcgtg ggcagcgcga tcgtcaaacg
     2821 catcgccgcg caccagcagc agcccggcat ggtcacgcag gtcgcgggtt tcgtccgttc
     2881 cctcaaggcg gccatggctt ccagcccggc atcagtgtga taatctcgtc gcgagtctga
     2941 tccccgcaac gcaatcgtgc cggtatttcc atcggggagg gggcggacgg ctacgtcgtg
     3001 atgggggaga gaggctagcg ttttggctgc ggaggcatga acgcgatcat ttcgttggcc
     3061 aattcggtgg cgacatcctt caagttgggt ctgtaaaaca tatagctgct ggatttttca
     3121 tgccgcgcct tccagaccgt cgtgccggtt gtggcatcga tcagccgcaa gctgagtccg
     3181 acgcggccga cgttatcgcc ttccttgcgg gcatattccc aggcattcac ccgaacgacg
     3241 atgaatgaat cgacgttcaa ggctttcccc agcttgatgg cggcttcctt gtcggaatgg
     3301 ccggtcattt ccaggcgaga gaagtagaac acgagcgaat cgaacgcttc tttcgaggcc
     3361 tggaacacat ccgtcacggt ttccggacta atgacacgct cgacatgggc caccttggtc
     3421 aacgccgccg acaggacatc ctgaatgtct tcgcgcgcgc tgtcatactg actcgccatc
     3481 ggcgggagca cggcgatcgc ctgcggccga aacaccttcg cgctcgggcc ttcccagacc
     3541 tcctgtaaac cgccacagcc ggcgatcagg agcgaaagaa gacaaataaa gatgctgcgt
     3601 cggagctgat gaagtttgaa catgcgctca gtataccatt taaaatgtcc aggccagcgc
     3661 ggcggagcca atcccgccgc cttcgttaaa gtcgtagccg ccggcaatat ccaaacggaa
     3721 tgagtagatt ctgatgccga gtccgcccgt cggcgtaaag atcgatcccg catcctgcac
     3781 gttcttgtat gtccccactc tgaacgaaag aaactccgac agaatcgttt gttccattcc
     3841 gaggctcaac acctgactct tgaggccggg tgtaagcgtt ttattggagg tgatatccat
     3901 atccgccgta atcgtcatcg tcgaccaggg attcaccgcc actccaccgc gcacttgcgg
     3961 cgtcaacttg aattcgctcc cgttcggcgc gtcgaacgtc ggttggttga gatctttccc
     4021 gatcacgccg aaccgcagcc atgaagaggg ccgatagatg gctccgacat cgatgccgta
     4081 cgacgttgat atcttcgctt tgccaaggtt gtcgagcagg ttaggatcct ggccgccttc
     4141 gaggttggcc gtactggtgt aggcggcgcc ttgaatcagc ttggcggtga caccgagggc
     4201 aaacgtctta tcggcgaacg cataggcata ggagaaggcc gcttgccggg cttcgagtcc
     4261 ttgcaacgtc atttgaccgc gaatattggt ccccgtgagc accaccggtg aactgacaaa
     4321 ccctccggac gtgaagacat ccgatacgtt gaacccgaac gcatgttcac caaggtgtcc
     4381 cttgacgtag agacctccgg tgcccactcc ggagattgtc gctccgggct ggttaatttg
     4441 attggcaatg ccctgggccc ttgcctggtt gccggctgag gtatcattcg ggttgaagtc
     4501 ctggaggtcg tgtaaggcac tggcgatccc caatcgagca gttccctgcg cggtcccttg
     4561 aatccgaatg tccatggatt tgaccatggc cagcccagcg ggattccaat aggtggcgta
     4621 cgcgtcggtg gtcacggcca ctccggcccc gcccatgccg gctgcacggg ggccgacgac
     4681 ggcaaattcc acggccattg ccggcgttgc aaccagggag agacagagat agagaaggat
     4741 ccgaattggt ccggaagttc gcgtcatgtc gcttggttca tgcagattca tggaagttac
     4801 caagtctatg taaggattga ggaatcgtca agcattagta ggttcaggat gctcaaaaag
     4861 ctgagaagag agactttttc agtatcctgt tagatgatct gaccgtcctg catatgtacg
     4921 atccgatcag cctgggccga cagcttatcg ttgtgggtga caatcacgaa ggtggtgccc
     4981 cgcgttttat tcaactcgcg catgaggctg aacagtgcat cgccggtgtg ggtgtccaga
     5041 ttgccggtcg gttcatcggc aagcacgaga tccggttttt ggaggagcgc ccgggcgacc
     5101 gccacacgct gctgctcgcc gccggacagt tcgcccggct tgtgatggag gcgggctccg
     5161 agtccgactt cctcgaggat cgccacggct tccttctcga tctcggccgg ctcccgtcgt
     5221 tgaatcaaag ccggcatgca ggcgttttcc agcgccgtga attccggcag gagatgatga
     5281 aactgaaaga cgaagccgat ccggcggttg cgaaaatcag cctggtccgt ttcggatagc
     5341 tggaagagat ccctcccctc atacagcacg gttcccttcg tcggctggtc gagcaacccc
     5401 aggatatgca gcatggtgct cttgccggct ccggaggcgc cgacgatcgc gatcagttcc
     5461 ccgcgtgcaa tggtgagatt gatgcctttg agcacttcaa ggtggtgctc accgatgaca
     5521 aacgacttat gcaggtcggt gacggtgatc ataatgagag ccttcagcgc tcagccttca
     5581 gccataactc ggagctgact gctgagagct gtcggcgagt agcgtgacag ctattcatag
     5641 cgtaacgccg ccaccggttc gagcttcgcc gcttgaaacg acggatagac ggttgccgca
     5701 aagctgatca ggatggccga gcctgccacc aggagcacgt cttcagccag cacatgaacg
     5761 gggatcttgg agatgtaata aacggtcgga tcgaacgtcc agaaggtttc gatcagccag
     5821 agaaaggtgt agcccaacgg aatgccgatc gccgtaccgg acagcccgat gatcaagccg
     5881 ttcaacatga aaatccggcg gatgctcttc ttggtagcgc ccatcgcttt gagaatcgcg
     5941 atctccttct gcttttccgt cacgatcatg gtcagggtgc tcacgatgtt gaaggaagcc
     6001 acgatcgtga tgagaaccag cagaaggaac atcatcgttt tttccagctt gagcgccgag
     6061 aagagattcc ggttcatctg catccagtct ctcgccccat aactgaaccc gagtgcctgc
     6121 tccacccggc gggcaatctc gccggcgcga aacacatcgg tgaccttgac ctcgatcccc
     6181 gtgaccgagt cgcccatatt gaagaatttc tgcgcttccg cgagatcgat ataggccagg
     6241 gacgaatcgt actcgtacat cccggactgg aaaatcccgg cgacggcaaa gatgcggatt
     6301 ttcggcacca tgcccatcgc gctgatcggt ccggcagggg agacgaccgt gattgtatcg
     6361 ccgacgaagg tgccgagccg gagcgccaat tcttttccga gaatgatgcc gggtttttcc
     6421 gaaggctgcg gcgggccggt cggattatcc ggaggaggga ggctcacctg tatgggcttg
     6481 ctcaggtccg ccagctgccc cgttgccagg ttcttcgcca gttcagtcac cttcccttcg
     6541 cgctgcggat cgataccgcg gatcaccact ccctgggcgc tggtctgagt cgtcagcatg
     6601 acctgtttca gaataaaggg cgtggccgct accacgtcgg ggacggcttc aacctgctgg
     6661 gtcgtcgcat tatagttgct catgccgtct ctcatgcggt cctgcacgac aatatgggct
     6721 gtcgtcccca gaatcttggc ctgaatatcc tccttgaatc ccgtcatgat ccccaccgtg
     6781 ccaatcaatg ccgcgacacc gagggtgatg ccggctaccg acacgaacgt gttcagggaa
     6841 atcgtccggt tgcgacgctt ggcgcgcaag tagcgcagcc cgacgaagat ttcatagggg
     6901 agtgtcacgg gtcgtcattt ctccggccgc aattggggga agagcaccac atcgcgaatc
     6961 gaggcttgat tggtgaagag catgatcaag cggtcgatgc cgatcccttc gccggcagtc
     7021 ggcggcatgc catactccag ggcgcgcaga aagtcctcgt cgaccagatg cgcttcttca
     7081 tcgcccgcgg cccgctgcac cgcctggcct tcgaagcgct cgcgctgatc gaggggatcg
     7141 ttcaactcgg aaaaggcatt ggcgatttcg cgcccggcga tgtagagctc gaaccggtcg
     7201 gtgagggcgg gattggaatc cttccgtctg gccagcggcg aaatctcgat gggatagtcc
     7261 gtgatgaagg tcggctggaa gagattcggc tcgaccgttt cctcgaaaat ttcgttcacg
     7321 atgttgaaca gcggccattt cggatcgacc gggacgttca gctgtttggc cgccgccgtc
     7381 gccttttcct tatcgtgcag caccgactca tccagcttgt tcacctcgag gatcgcctgg
     7441 tggtaggacc agcgccgcca gggaggggtc agattgatct ctttgccctg atactcgata
     7501 agcgtctttc ccagaatctg ttgtgccagc gaggtgatca actcttccgt cagaataatc
     7561 agatcgtggt agtcggcata ggaaacgtag aactccagca tcgtgaattc cggattgtgg
     7621 atcgtcgaga tcccctcgtt ccggaagttg cggttgatct cgaacacgcg ggggaagccg
     7681 ccgacgatca gccgcttcag gtacagttcc ggcgcgatgc gcaaatacag atccaccccg
     7741 agcgcattgt gatgcgtcac gaacggcttg gccgccgcac cgccgggaat cggatgcatc
     7801 atcggcgttt ccacttcgag gaatccccgc tcgatcaaaa acgagcgaat ccccgagatg
     7861 attctgctgc gagtcgcaaa gataccatgc acttccgggt tggcgatcaa gtcgacgtag
     7921 cgctggcggt agcgggtctc gacgtcggtc agcccatgcc acttctcggg caggggacga
     7981 agtgccttgc tcaagaaggt gagctgatgg acctcgacgg tgaactcgtt ggttttcgtg
     8041 cggaagagag tcccgctcac cccgatccag tcgcccagat cgagctgctc ggcgacggca
     8101 taggcctgct cggacaacag atccttcttc aaataggtct ggagacgatc ggatccgtcc
     8161 tgcaacacgg caaacgctgc tttgccgaac cggcggagcg cgacgacgcg cccggcgatc
     8221 gtgcagggaa tcttttcctg ctccagggtc tctttccgct tctccccatg aagcttgatc
     8281 aattgcccgg cccggtcctt gacctcgaag cgggttccgt acggcgccac gccggcttcg
     8341 cggagatgat cgagcttctt gatccgttgc tgtcgctgtt cgttcagttc ttccatgatc
     8401 aatcgcctcg agccatcagc gttcagccat ctgctgccgg ccgttagaga tcatccggct
     8461 cgctgacggc agagagctga tcgcttcctt tcgtgagctt ctttttcaaa tacgcttcaa
     8521 tgaatccgtc cacatcgccg tccatcaccg ccgccacttg cccgacttca taacccgtcc
     8581 ggtggtcttt caccatctga tagggctgga acacatatga ccggatctgg ctgccccagg
     8641 cgatatcttt tttctcaccg acgatagcgt tgaactccgc ctctttcttt ttctgttcca
     8701 gctcgaagag ccgcgccttc aagattttca tcgcgccgtt acggttctgc aactgcgagc
     8761 gctcgttctg gcactgaacc acgattccgg tcggcatgtg cgtgatccgg atcgccgttt
     8821 ccactttgtt gacgttctgc ccgcccgcgc cgccagcccg gaacgtatca atccgcaagt
     8881 ccttgtcctc gaccgccacg tcgatatcct cgttgagctc gggatacacg aacaccgatg
     8941 caaacgaggt atgccgccgc ttgttggaat cgaacggaga aatgcgtacc aatcgatgca
     9001 cgccggcttc cgctttcaaa tagccatagg cataggggcc ggtgacggag atggtcgcgc
     9061 tcttgatccc ggcctcatca cccccttgaa ggtccagcgt gtccactttg aatttcttgc
     9121 tctccgccca gcgcacgtac atacgcagca gcatttgggc ccagtcctgc gactccgtgc
     9181 cgccggcacc aggatgaatc gccacgatcg cgttgttctg atcgagttcg ccggagagga
     9241 gatgctcgac gcggagggcc gctaggagcg gttcgaattg atcgagctcg gcggcaagct
     9301 cccgttcgag tccggcatca ccgctttcct gcgccagctc gagcagggtc gtcacatcgc
     9361 tcaatttgga ttcaatgtcg cgccactggt gcagctcgcg ctccagcttc actttcttgc
     9421 ggctgacgcc ggccgccgcc cgcgcgtcgt tccagaaatc cggctgcccc atcttggctt
     9481 cgagttcttc caactcggcg gtcatgcggg cgaggtcaaa gatgcccccg aagttcggca
     9541 acctgctcac cgacggagcg cacacgaata cgaatctcgt ccaacatcga agaatccttt
     9601 cgacttacac gggggttgcc gcgatggcat ctggttcagg tcccttcgta ccgaagaggc
     9661 cgaacagggt caatagaagg cagattatca cacagccgta ggcaaagaca tccccgtatc
     9721 gggtgtagaa cgttgcgggt tgccgcaccg ggatggtggc ctgcaaggcc gcttgggtga
     9781 agatggacgt ggcctcgatg atgcgcccga acggatcgat gaatccggac acgcccgtat
     9841 tggctgcgcg ggcaaaggcc aggtgattct caaccgagcg gaacaccacc atcgagaaat
     9901 gctgcgacgg cgcggaggag tcgccgaacc aggcgtcatt ggtgatcgtc accagaaatt
     9961 ccgcgccgct ggccgccatg cgccggacga gatcggggaa gatcacttca taacaaatcg
    10021 ccacgctgaa tttcaccgga cgagggtcgc ttcccgtgct cgaggccttc ggttgaaaag
    10081 acaggatcgt cggccctggc ccggcctgaa aatcgccgat cccttccacg agtttatcga
    10141 ggaaaaagag caccgaggac ttcagtggaa tgtattcgcc gaacgggacg agatgctgct
    10201 tgtcgtagcg gcccaataga tgtccatcgg gagaaagcag gtaggcgctg ttgagcagat
    10261 agggcgtgcg atcggcgtgg taccggaccg ccgggcttcc gaagagtaac ggcgccgacg
    10321 cgcgattggc caacgcgacg agctggagtt gatagaccgg ctcccgctca tacacgaagg
    10381 gcgtcgcagc ttccggccaa atgacgagat ctgctccata gccgaacgtt cccgtgagct
    10441 gctcatagcg ccgcagggtt tcctcacgaa agccctggtc ccatttgacc gcctgatcga
    10501 tattgggctg gactacaccg atctgtaacg tcgtcttcgg cggtccaaac ccttcgtgca
    10561 cgatcaacga ggtgctgtag gcccaggaaa ggcccaccag aatcgccgat gtgctcacca
    10621 gttcccaggg gagcctcgcg ggacggaatt tgcggaacag cggcatcaac cacagataca
    10681 actccgccag cgccacgttg gtcagcacga tgaggaagga aacaccgtag acgcccaggt
    10741 gatccgcgat ctgaatgaga tccagctgcc ggtattgcga atagccgagc aacgaccagg
    10801 ggagcccgct gagcagatag gtgcggagga gttccagcgt cacccagagg cagggcgcgg
    10861 cgaagaggcc atagcgcggg atgagcgagc ggaaccagac cattccggcg ccgtagatgg
    10921 ccacgaacag accgaggtaa gccgtgagca gcagcaggat gccgtagctg atgatgagcg
    10981 gcaccttgcc ataggtgtgc atcgccgtca ccacccaagc catgatcccg gtaaaaccga
    11041 tcacgccgga gagccagcct agccaaaagg cctgcctctt gctcgtgccg tctaaggcga
    11101 tatgcagggg gatcagcgcc acccaggcca gcaggcccat atcgaatttc ggaaaacaga
    11161 agggaaggag aaccccggac acgacggcaa gcacgatctg gcgaacgggc cactgaagca
    11221 tccggctaac ttagcggaag gttccgtgcg atgcaaccgc acggggcggg caacgcgacc
    11281 ccacgccgct agagaggtgg tgcacgggct gaatcagtgg gtcaagatgg agagcggccc
    11341 ctgctggcgc tcaaaatctt cctcttccag ttcgccgacc gctccgcaat ccgcttcgtc
    11401 caattcaaca ccgaaacggt gttcgttggc ccaacggacc gtggctttct tgatcagcag
    11461 cgcccgctga tcggcaggaa tcaacaacag catgagttcc atgccgacgt gcaccgaatg
    11521 ggtgccgtgg agacagccgc cgctcgtggt cagattttcc gtgacaccgc tggcgtgaaa
    11581 ggccccgtcg gaataataca ccgcacaggc gacgggcatc cggctgctgc ggcgtttgtc
    11641 tggttgatac gtcatgatac tcgtgctcct tgctcaggcg gccggtctca ccagtccggt
    11701 tttgtcgggg tgctcagccg gcatcgccca ccacaatatg aggagcccag ccattccgat
    11761 tcccactacg ttggaaaaga catatccgct atcgacgaca cgtccggaac gaaggcctga
    11821 cagatttcca tgacgaggcc gaacgcgaag gccgcggaca ttccggtggg cagcgcatag
    11881 tgttgaggcc acccgcgctg gagcagtccg acggtcaaca accacgtcag cagtccatgg
    11941 gctggtgcat acgacattgc cgttacagtc agcgggagca tgacgatgag gccgcggaaa
    12001 aatccgacac caggcgaaat catcgcgtcc cgtccaagca gcaccatgta gaacaagtga
    12061 aaagcgatac gcctaaggaa attcggcctt cgaattcccg aactatgatg ggaaggtaca
    12121 agaacagcgt accgagaaac taccgcacaa tagtagggga ggccggtcga cgtggagaat
    12181 cctgactcac gtcatgtaga atgcgcgagc cactgaggca tatcaaccga cacgcacttg
    12241 atggatagat cgtcaatgaa gagcctacct gccaaatcaa cagcttggag ctgctctctc
    12301 gtttgccagt tgaaagtccg tgagcaaggg acagaaccat ccaagagaca gaatgttcca
    12361 gacacatata ataggagaca cgggggcagt actccttcca tctttcgagg ggcaaatgcg
    12421 ctaggcatca agcctgatga tttcttccaa gggaagaggt attcttccgc cccaacattc
    12481 gtacttgttc accatcagat cgaaacattc ctatcggtca gaagaggatg acggcactat
    12541 gaacgcacag acggtaatcg gaatcacctg catcgccatc atgtccagta gctgcgtcat
    12601 gaagagcacc tacgatgcgt cggttcgaga aacggattcg ctcaaggccg agctcgaacg
    12661 agccaaagaa gaacagaggc tgtatgccaa gcaggtgaag gaactggaac agagcaacaa
    12721 tgacacgatg caggatgcag aagccacgct cacggcaacg atgagagccc agaccgatgt
    12781 ggaagcggag cgccggctcg ccgaagaaca agaagccaag ctcaaacaga aaatcgcgca
    12841 actgatcagg cagcatcatg ccatgcgggg tgaaatcgcc gtcgcgagag aaaacggcgc
    12901 agcgctgcag gaactcgtcg aagtctacaa gaagaaaatc gcggaattgc cgagagacaa
    12961 tgtcgtgtcc tcttcgccaa acatgacgcc gacttcgaaa ccgttcgatc ccgcgttact
    13021 tcctccggtg caggatttgc ctcccccagc gccggcagtc gaaccatcca aaccggcgcc
    13081 ggcaccggca ccatccacgt cccccgtgcc ggccaagcac aaacaggaac cggccgaatc
    13141 aggctggcta accacgatca aggaatgggt cgtctcgctc tggcgatcga ttttttcctg
    13201 aacaggatga tgcccccgtg cgggagcggt cctgccatgt cgtcatcacc agtccttccg
    13261 tgatgaccca gccgtcaagg tgattttcaa gttcagacat tgtatgttga cgtcagaaga
    13321 aactcgccga atctgagcca tcatgtgaga gggagagatc taatgaaacg agcatggatt
    13381 gcagcgggat tgtgtctgtc catcgccgcg gccgggtgcg gaggcggcgg gacggccgag
    13441 gtgagagaag atcgcctcac ggtcggcaag gtgcagggag agatcaaagt cggcttggcg
    13501 gccgctcagg ttgcggagtt gctcggcagc ccgaacatcg tcacgacgga tgaaaagcgc
    13561 cgcgaagtct ggatctacga caaagtctcc accgatcggg tggacatggc ccattcctcc
    13621 tttgccggca tcatcctcgt gatgggcggc acgtcaagcg agagttccag ctccaccagg
    13681 cagcgcaccc tcaccatcat catcaaatac gacgaagaga agaaggtccg cgactttgcc
    13741 tataactaca cgcagttcta accggagctg cggtcgcgtg atgtcgcgat ggctcatcgc
    13801 ggcgatctgc ctcctcttct cgagccaact tgccggatgt gtgacgcaga gccagccgga
    13861 agaacttttc caactgtctc cggagagtcc gaagcatcgc gccatgcaga cgcgcatgtt
    13921 cgaaacctcg gacgagaagg agctgctctc ggcctcggcc gcaacccttc aggacctggg
    13981 gtttgagatc gaagagagcg tctcggacgt cgggttcctc cgtgcagcca aagagcgaag
    14041 cgcccgcgag tggggccagc atatcaatcg gtttatgatg gcgattgtca ccaccccgct
    14101 ggctatcatg gaggccggac ccgtcatcct tcccatcgat ctccatcaga gaattgcggc
    14161 ctcactgatc acgcgaccgc tcaacccgga gcgatcacgt tacgaagtgc gcatcatgtt
    14221 ttatcgggtc gtatggaaag gggagggtgc agccggtcgg agctcgtttc caccgggtga
    14281 gcagcgttcg gaaatgattc gtgacccggt cctctaccag caattctttg ccagattatc
    14341 caaagccgtg ttcctcgagg cgcacaagat atgatgcgtc cataccagaa cgcgaggcgg
    14401 acccttggcg cgtggctggt gctcgcagcg agcctgcaac tctatgggtg tgccccggaa
    14461 cccagccagg acttgttcgc gccgacggaa gcgcaaatga agctgcgcag cttccagacc
    14521 cgcaccttcg acgtgagcga ccgccaggtg gccctgcgtg cagttatcgc cgtcctgcag
    14581 gatttggggt tcatcatcga gcgcgcgaac gaaccgctcg gcctcgtcac cgcggcgcga
    14641 ttctccgagc ccaactatct cgaaatcgtc ggcgtcaccg tcaccgtgcg accgcagggg
    14701 gagggccgca tgatgattcg ggccaacgcc atccataaca acaaacccat cgaagatccc
    14761 aaggtctatc agaacttttt cgcttcgctt gagcggtccc tcttcatcac caaagaatga
    14821 ccatcttgcc gatgcgcgcc cggagagaca caacgcgtcc gaccatttca acgccggctg
    14881 tccgtcatct ggcgatgggg agtgtcgtgg ccctgagcct tttcgcgcag gggtgcctgt
    14941 cctacgaatg ggcacagcaa atccaatcgg actcccatga tcaggtcctc gcatccgagg
    15001 cgagccaggt taagcttcgg gccgcacaaa cccgcagctt cgacaccacc gatcgcacgc
    15061 aggttctgag agctgcggtg ttcgccctcc aggacttgga ttttcagatt gaggtcctcg
    15121 acgaggatct cggcatcctc tcagggaaaa aattcgtggc catcgagcgg cccaatctgg
    15181 gttatgaccc gagctaccac ttatatgatg atacaggact gctgctcttc aacaggacgt
    15241 actttggctg ggggccgttc tggcatcgca gcgacctggt ccgcatgacc gtgaccgtgc
    15301 gcaagcgcaa tgaagcccaa ctcatcgtcc gggccagcgc ccagttctac cttcgacccg
    15361 tggaagaacc gggcccctac caaatgttct tccgcaccct ggagcaatcc ctctttcttg
    15421 aaggtcaggt cgtcgagtaa ccgcgcctag gcctcctgca tccgcacgaa ttccctggcg
    15481 cgacgcagat cgtcacgcgt gcagagcttc agctccagca agagatgctc caatgcagcg
    15541 acgcgggccg tcatctccgg cagcacgaga tcgatctgct cgcgcaattc cacgaaccgg
    15601 cggttcaacc ccgcatccgc tcgcacggcg gctgccctgt tcttcttccc gccaactggt
    15661 cggcggcgca ctcggttccg cgaatcaagg ttctttgtgg ccatggtacc ctcctgattg
    15721 tgatatcttc tgtgcccggt ttcgtagaac cggtcacata ccattccaac tcaatggttc
    15781 aatccaatgt gtctcggacg ctcggagaat cgtacgagca cggatgacgc agaccaagct
    15841 tagaggaaat gggaaagggc gtccagagcc actttgtccc cccttgactc acctatccga
    15901 aaggtccaga cttcttgaaa ttcatccggc cgcgcgttcc cgctcctcac cgcccgacaa
    15961 aaggggaact cccatgattc aaactatcct gagcgctccc acaacacaaa aaaccgcagt
    16021 ccgacctaca accatcagcc cctgcagaca cggccgcatg gtggatgaag tccgtaccat
    16081 aaatggcgac agcacgggcc agctcatctg catggaatgc ctggccgtgt ttcctgatcc
    16141 gctcgatcag aaacttgcct cctaggatca tgcgctggtt tcgccgcttc gcagaacaca
    16201 acacaccaga aaaaggagag agaccacccc tgcgttccgc tcccctcgca caatcgtaaa
    16261 acatctctca ccctcagcac ttcttaggcc tctcagcatt cggttctcag ctcgtattaa
    16321 gcatctcagc tctcagcact tcttatccgt tcgcctcacg cctcacgtct tcccgccctc
    16381 agcactctca tctcagcact tccgggacgc gcacggcact tctcaagatg atctgacccc
    16441 taactcagaa ctactttgcc cgtcgtccgt tggaggtcat gacagacaaa ttcaggccct
    16501 cttcgtcgcc atccgccagc tcatgtaaga gcctgcggca aaatcccgcc gcatcggatt
    16561 caagacctga gaaggctcgt cgtctgtccg gatcgtctgg tctcccggct tcatcttgtc
    16621 actgttcgag tggaccaccg ttcgcgggcg cgagtttcgg gttgtactag ccgattacag
    16681 ccattaagct ggatggggag ttgcgccagc tggttcagag cgtgaaaaaa cccaaaacca
    16741 atatatggac cctgctcctt gcggtggcat tcgcgctcgt catcacagtg ctggacttgc
    16801 ttccctggtt caagggcgtg ccatggaatg tttgcttcgt cgtcccggtc gtttgggtcg
    16861 ccttgtggtc cgcggaggac gatgtacttc cgctcatcac cgtggggctc atcgtcacga
    16921 tcctcacgat ggttcgaggc gttgtcttcg gcggtatcct ggatccgacg ccgatcacgg
    16981 accgcctcat cgtcggaacc atcatatggc tgaccgtcct ccttgccgtg ctccgaaaac
    17041 gtgctcgccg cacatttaat tggatcaccc tcgccggtag acagtgggaa ccagacaaac
    17101 ccgacaacga gaaaaaccag agtgaccaga caaggtagat agactaccct ttgtcactca
    17161 cgcccgggcc ttcgccagac aacagtgttt atatttgtta ccgctgccgc aggggcaggg
    17221 cgcattgcgg ccgatcccct gacggcgccc gggcaccaac ggcagtatcg atcccgtatc
    17281 ctgatgaaac atatctcgca tgattgcgta caaggcggga tcgcgccgct tcaacgactc
    17341 cggcgacgtg aagaaatatt cggccagcac cgcgaagaat tcatgttcgc tcgtgtaggc
    17401 ataggcgccg acgcgggcgc cacgcctcga cggatgggcc agttcccggg ccacgtatcg
    17461 cacccactgc cgcaccgcca tccagggcac ttccggaggg aggccatact cgttctcttc
    17521 cttctcgacc agatgcgcga actcatgcac gcccacattg tgcggacctg cctgatggtt
    17581 gaatcctgcc agcagcgcgg gcttcgagag aatcatcact ccgctcagat ggtgcaggcc
    17641 gaccattccg agaattgtct catcggcccc gccgcggctc tgataggcat cgtcgaatgc
    17701 attcggatag atcagcactt cacgcagccg atggtattcc caatcgtgga agccgaagat
    17761 cggaatcgcc gcactcgccg caaccagcac ccgaatcgtt tcgtccacct ccgtgcgaat
    17821 acccgtgatc cgaacctcat ccagaaagat ctgtaccagt tggcggaagc gtgtcttccc
    17881 ttcatcatcg agcgccgcga agaacgcgac gtgcgtgcga agaatctgct cccgctcgtc
    17941 gggaaacggc tgccgcatca ccgccatccg ccggagacag cgccgccgca cccaccagta
    18001 agtgaacggg cttagggcca gcagcagcca caaagcagga gagtgccagc ccaaaaatcc
    18061 cgccaccacc gccaccagca cagcgccgac aatcgcctgc cgccgattct gacgattggc
    18121 ctccggtgtc acgagcatcg gcgagatctc caggctaaaa gtgtagccgc gtcaggattc
    18181 tactccggat gaaccatcgg ttgtctcgtt tctctcgccg aaatagtccg tgcgattttt
    18241 ggctaccata ttaggaacgt tcatctggtc tcttttgttt gtctggttca tgtggtttat
    18301 ctggtttgtt tggttcatgt ggtttgtctc gtttctttgg tttaccgaac gaaataaacc
    18361 agatcaacga aacaaaccag tcccgccagt ctcgcctttc ccgctcgtcc cgctcgtccc
    18421 gccttccgcc ttacccctca cgtcttcccg ctcgtctcgc ccgtctgctt gacgttcgtg
    18481 ccatcccgtc gtaatgtccc tgacgcatcc tgtcgctatc gccggattgg tgagtggaag
    18541 atgaaacaca aaccctcggc gcgagatctc ctccccttcg gcattatcgc cgcagctttg
    18601 ctgtccggcc tgatcgtcca atccctctgg tcaaatcgag tgtgggtaaa cgttcccctc
    18661 cattccaccg tggaagcgtt gggtgggttg gcggcggtcc tgatgggcct ggtgttactg
    18721 gcgcggaaag cggagtggga agatgagagg ctccaggggg ttgcgagcgg tttattggga
    18781 atggggatac tcgaaggctt tcacgcggtc tctactcccg agcatggctt cgtgctgttg
    18841 cgcagtgcgg ccagtctcct cggtggcgtg gccttctcgt ttgtgtggca tccgcggggg
    18901 atgatcggga agcggaccag agaactgatt ccctacctgg tcacagccgc aaccgtcgcc
    18961 ttcgggattc tcgtcctcgc ctttcctcat cacctgcccc gattcacggt ggaggaccaa
    19021 ttcgcgccga ttgccctcgc ggtgaacggt gtcgccgctg tcttgttcct caccggcgcg
    19081 gcgggttttt ggctcgagtc cggacgcacc ggcaggccgg aacatcagct cttggcggcg
    19141 ttggcgatcc tgttcggact ggccgaaacg atgtttttgt tctccacccc ttggcagagc
    19201 gaatggtggg tctggcattt agtgcgattg gccgcgtacg tcctggcgct cacgtatgta
    19261 agccgcggct acgtgcggat ggtcggcgaa acacgaatcg ctctggctga ggcgaagcgg
    19321 tcgggacggc gcctggcttc ccaatacgca gtgacccggg tattggccga gtcggcgagc
    19381 ctgaaagagg cggctcaggc aatcctgcgt gccctcgcgg agaacctcga gtgggaatta
    19441 ggcatgttct ggagcaggga cgaacaaatg cagtgcctgc gctttgtcga cctttggcat
    19501 gcgccgcatg taaaggcaat cgagttcgtc aacgacagtc gggggcggac atttcaacgc
    19561 ggtgagggac tcatcggtcg tgtctgggcc accggcaagc cgatttggat tccggatgtc
    19621 gtcacagacc ccgattttcg ccgggctccg atggcggcga gggccggcct tcacggaggg
    19681 tttgcgttcc cggtgcgcaa gggcgagcac acgtatggcg tcattgaatt tttcagccgg
    19741 gagagccgtg agccggatca ggatgtgctc gaaatggtcg cggacatcgg gatcaaggtc
    19801 gggctgttcg tcgatcggaa gtggacagaa gaggagctcc gtcgaaccga ggctcgactg
    19861 gtggaggagc agaggctcgc cgaagtgtcg agagtattag gagacatcgg gcacgatctg
    19921 aaaaatatgc tgatgcccat tgtgaccggg gcggggctcc ttgaagaaga actgagcgaa
    19981 tgcttcgcaa agttgcccca acccgcgatg agtgccatga aacccagccg agatctgacg
    20041 catgacctca ttgagatgat caggcaggga ggccggcgca tccacgaccg ggtcaaagaa
    20101 atcgccgatt ccgtcaaggg tctgacgagg ccaccacagt ttgcgccttg ccgcgttgcc
    20161 gacatcgtgg ccagcgtcta cgcgaccctc aacatccttg ccaaggaagg ccgggtggcg
    20221 ctccgcacag agggcctcga aacgctgccg gtcattcgtg ctgacgagaa ccgactcttc
    20281 aatgccatct acaacctggt caacaacgcg attcccgaag tgccatcggg aggatcagtg
    20341 accgttcaag gccgcgcgga ccaatcggga aagaatatca tgctgtccgt catagacacc
    20401 ggcaagggga tgtcccccga agtccgggac agtctcttct cgtaccaggc gatcagccgc
    20461 aaaatgggcg gaacaggact cgggacgaag atcgtgaaag acgtggtgga tgcccatgga
    20521 ggcagtatca cggtagaaag tacaccaggt gtcggaacgg cattccatat tacgctgtcc
    20581 gttgatggtc ctcccgctcg tactccaaac gcttcgccat ctcctgtaga gacgggccgc
    20641 atgacgttcg gttctgcgtc aaaggaggaa catcttggca gatgagaaaa cacctccggt
    20701 tccgacgggg aagaaccctc ccaaggggca aacaatatgg gaatgtttcc attgctcccg
    20761 ccgatatttc accgaacagc cgccgacgaa gtgcccgttc tgcctagccc cagtcaaaac
    20821 atgacgtggc atcacattgc ccgactcgcg cccttctcgc tagtcccgct ctcatatatc
    20881 tcagcactca gttctcagca cttcctcgat gcgtggcgtg atttgtgccc ctcgcggctt
    20941 gcccaagtcc gtttaccttg ttacactgca aaattgcgaa acggaaacct gacgagcgca
    21001 ggagcagagc cgtatcgtgt tttcaccaag gaggttgtat gaatcgtcat gttcaaacgg
    21061 tctgcctact ggctatcggc tgtatggccc tgtggacagc gggatgctca agcaaaggtg
    21121 taaaatcgga cggcagcacc ggttaccctg cgccatcgca gagcgagccc gtgcctaact
    21181 tgtccatgag cgatccgccc ggcacagaac ggctgaactc cggcattgcc gtggccaagg
    21241 cggacccggg cgcggcaaga cggcaaatgg acgaaatgcg agcagaacaa agcgcaacgg
    21301 cggcctccgg cctgcgcgat gtgttttttg cctacgatag ctggtctatc aatgaggagg
    21361 ggcggcaggc gctctcccgc gatgccgagt ggctgcgcac gcatccgacc gttcagttga
    21421 aagtggaagg gcactgcgat gagcggggaa gctccaccta caacctcgtg ctggcggaga
    21481 agcgggcgaa gtcagtccgc aattatttga cggagcttgg aattcggaag gaccgcttcg
    21541 tcgtcgtgtc ctatggcaaa gaacgcccgt tttgtaaaga gcgcagcgaa gcctgttatc
    21601 agcagaaccg gcgaggccat ctcgtcgtca agccatgaca gtggcgagtg atcgcgtgat
    21661 gcataggtag aacctacccg agacggccca ggctctgggt cgtctcgggt aggtttcctc
    21721 ccctctcgct
//
LOCUS       DATIFR010000130        10202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2769894, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000130 DATIFR010000000
VERSION     DATIFR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769894"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VLD60_07470"
     CDS             <1..1265
                     /locus_tag="VLD60_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSB44833.1"
                     /translation="LHPQHDNASSLQRDADIPVRGTEEERGDAQQAEAKEEALKSLFV
                     QGHYTVLPLPIFAYSRNESYWVGAVVPVLQSNAKDELQDIYAPFYSHNRYVGETLGIY
                     YYGYPSDTTQYSVTADYSTKTQRDIDLTYKNVGVAGGRYILAGRVSWFKNPFRRLFGI
                     GNQTSESDETTYTSREGLVDLTAGIHLNEDVALMWTEHYHDVRIDTGIVETLPEAKTQ
                     FGQLPGMEGANILGHKFTARYDTRDKQLISTRGTYVNLSVELNQNLQHSGSNPWVRTT
                     LDARHLFPHYDDQLVFVTRFLVDTVNGHTQHIPFYERPTLGGETTLRGFGQSRFIDDT
                     AVLINFEERIPVKQVKVFDYLIDLEVAPLLDIGRVMRGFSTRDIKDFQVNPGIGLRIL
                     ARPNVTGRLDIAYGRDGANVFVGLDYPF"
     gene            complement(1391..1594)
                     /locus_tag="VLD60_07475"
     CDS             complement(1391..1594)
                     /locus_tag="VLD60_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44834.1"
                     /translation="MQRNPACQIFRIHVSARIEERSDGLSVLVRFGKHERTRSHNRPC
                     LIRMMALVQEKQDRVRIPSSRRV"
     gene            2588..3961
                     /locus_tag="VLD60_07480"
     CDS             2588..3961
                     /locus_tag="VLD60_07480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HSB44835.1"
                     /translation="MESLRRRLFIVLVLSGALFTSTVAVEAQGRSEAALSALERTPHV
                     PNQVLVQFRYGIAEEAKESVRSRVQAQVEDVVVAQERRSDFKGDLELWNLPAGLEIAR
                     AVRELEGDSTVEFAEPNWIYQHQAASNDPYYTNGSLWGMYGDGTSPSNPFGSQAGEAW
                     VNKTDCSSVYVGIIDEGMMITHEDLADNVWTNPYDPPDGMDNDGNGYIDDIHGWDFAG
                     NDNSVYDGSGDDHGTHVSGTTAARGGNGIGVAGVCWNIQLISAKFLGRNGGTTANAIK
                     AVNYVTDLKTRHGLKIVATNNSWGGGGYSQGLSDAIKNAGAANILFVAAAGNSGVNID
                     ITPSYPASYTLDNIIAVAAIDKSGNLASWSNYGSTSVDLGAPGVGIWSTVPNKRSQSS
                     YSSYSGTSMATPHVTGAAALYAAAHPTATAADIKQAILSSTVPTASLSGKTLKGGRLN
                     ICSASCP"
     gene            4335..4739
                     /locus_tag="VLD60_07485"
     CDS             4335..4739
                     /locus_tag="VLD60_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44836.1"
                     /translation="MTGVETLLWLVVAFAEGPDCLLQGSFHRLPDGPAFVREDGCAVH
                     SLEEGSYVVFWSESRWVAIKIPARARSLSYRWGRAMAFVNGNSVKVQHGTILNALQRR
                     PSARDQSKTDLISKWRMHDVRVTRSINRRTIL"
     gene            5158..5994
                     /locus_tag="VLD60_07490"
     CDS             5158..5994
                     /locus_tag="VLD60_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSB44837.1"
                     /translation="MTALGARVLGLAGMLCVMAMGSGCVAIEAGHEGVLVEQPFFFGH
                     GGVNPVPATTGRINLSPTTKIIEVDIRPVQYAEHFDIISAENAPVSFDAFMIAAVIEG
                     RSPELISRFGPNWYQNNVKEAFRTFVREEVQKYPLFQLTTDPTTRAKLQDAIAREVQT
                     KLIEKQNIPVRLNRVVVGSILPPKGVVEQTTQTIIQEQRKITMVEFQKAEESREKAER
                     QRGIADRAYRESLGLTAPEFVDLRRIEVQKEIVQHSPSALTVIMGLERVGINMPPLAA
                     EK"
     gene            complement(6050..6778)
                     /locus_tag="VLD60_07495"
     CDS             complement(6050..6778)
                     /locus_tag="VLD60_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44838.1"
                     /translation="MSLIRTAHIDVLFSALLLIPFPAEAAGAPNGRIEITGDYRYTAH
                     KSEPVAEAKALACREALRLAVVNSPIYREGTASVVDSPLLRDLADTLATNHVHDQQIV
                     EQTEQGRTVSCRVKGFLQAEESTRVIRTQLSSGPSPAEGLDQNRVLRLLSVKEEGRGT
                     IAIQYQALKRLDWLGTHYQGGLRESAEIMVDFYDEQGSLIRSERHPARRTPTGDDVMN
                     PGEIALLKVARPAAAKTYRVWLVR"
     gene            complement(6870..7784)
                     /locus_tag="VLD60_07500"
     CDS             complement(6870..7784)
                     /locus_tag="VLD60_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSB44839.1"
                     /translation="MTTNRYPTIRFGTSSWAYEGWQGMVYHRTYPKSRFSQDTLAEYA
                     SHPAVEAPLFRTVGIDHSFYRPAATTQLAHYAEQVPNDFRFCSKVWEEITIPAYANLP
                     RYGVKAGKPNPRFLDAGAFRDLVLGPAQEGLGEKLGPFIFEFQRWGTEPATFLEKLDQ
                     FLNQLPRGPQYATEVRNPALLTARYRDLLKAHGVAHVYNHWSYMPPLAEQHRSLEQTF
                     TAPHVVIRLLTPLGLKYETAVERYKPYDRIVQPQPRMRQETVELVKQAAAEGKSAYVL
                     VNNRSEGCSPLTVQALMEALNGPPSPPL"
     gene            7864..10113
                     /gene="malQ"
                     /locus_tag="VLD60_07505"
     CDS             7864..10113
                     /gene="malQ"
                     /locus_tag="VLD60_07505"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSB44840.1"
                     /translation="MPEQTEQTLLASLAERVGIATDYHDITGTRHLTADHTKRALLSA
                     MGFQVDSAAALAVAVRDWDEAPWRRPCDPVRIVQEEEAGAPVLCCLALEDGKERSVVV
                     EWQLRDEAGEIVQDERIGPGLTAAEVKFLDGHRHVRVELPAPKPLPLGYYQLALRADG
                     LVGGPVATMRLIVAPRQCYVPPGLESGQRFWGLALQLYSLSSSRSWGCGDFTDLNQFV
                     EWAGKSLGAGLVGLNPLHAIRNSAPFHTSPYAPVNRLALNELYIDLERLPEFYASDEA
                     QKQFRAPEFQVKLQAIREQRRVDYDAIAAAKRTMLDLAYRQFLKDAYAGNEPNLEPQT
                     ARAKLLERFIQAEGDALELYAIFQTLEEERRLIQSKDVTWHDWPKQFLSPGPAVREYG
                     KRHRKRIRFFQYIQWIATEQLTEVRRNAEQVGMPVGLYNDLALGAERNGAEAWMYQSV
                     LALDADCGAPPDALAPEGQNWGLPPVNPIALRASGYELFIQLLRKNLRLGGAIRLDHV
                     MALCRLFWIPKGKPSSEGTYVLYPFEELLAIVALESVRSKTLVIGEDLGTVPDWVRDQ
                     LAKARVLSYRVFYFERRDDGAFKLPSEYPQQALAVVATHDLPTLNGFWSGEDLQVRAG
                     LGAFCDDAARRQAWEERQRDKGLILSALQQEGLLPNEMGDDPATVPAMTQALCRAIHT
                     YLARTPSWVVLANLEDGLEELSQTNMPGTVESHPNWSRKYACRIEDLIGDGRLRELGA
                     CLRSTRPIS"
BASE COUNT         2151 a         2747 c         3043 g         2261 t
ORIGIN      
        1 cactccaccc tcagcacgac aacgcatcct cacttcaacg tgacgccgac atccccgtac
       61 gaggaaccga agaagagcgg ggtgacgcgc aacaggcgga agcaaaagaa gaggcgctga
      121 agtccctctt tgtccagggg cactacactg tcctcccgct tcctattttt gcatacagcc
      181 ggaatgaaag ttattgggtg ggtgccgtcg ttccagttct gcaatcaaac gcgaaagatg
      241 aactgcaaga tatctacgcc cctttctatt cgcacaatcg ctatgttgga gagacattag
      301 ggatctatta ttatggatat ccttctgaca ccactcaata cagtgtgacg gccgattatt
      361 cgacaaaaac tcagcgcgat attgatctga cctataaaaa tgtgggtgtc gcagggggac
      421 gttatatcct ggctggccgg gtcagttggt tcaagaaccc cttccgccga ttgtttggga
      481 ttggcaatca aacatcagaa agtgacgaaa cgacctacac ctcccgtgaa ggtcttgtgg
      541 atctcacggc cggcattcat ctcaatgagg acgtggcgct catgtggacg gaacattacc
      601 acgacgtccg aatcgataca ggtatcgtcg aaacgctacc agaagccaag acacaattcg
      661 gccaactccc aggtatggag ggcgcaaaca tcctgggaca taaattcacc gctcgctatg
      721 atacgcgtga caaacaactc atctcgacgc gagggacgta tgtgaacctc tccgttgagt
      781 tgaatcaaaa cctccagcac agcggatcga acccctgggt acggaccact ctcgatgcca
      841 ggcacctctt cccccattac gatgaccaac tggtcttcgt gacacgcttc ttggtcgaca
      901 ccgtgaatgg acatacccaa cacattccat tttacgagcg ccccacgttg ggaggggaaa
      961 caacacttcg cgggtttggt caaagccggt tcatcgatga taccgcggtg ctgatcaatt
     1021 ttgaggaacg tatccccgtc aaacaggtga aggtattcga ttaccttatt gacctagaag
     1081 ttgcaccctt actcgatatc gggcgagtca tgaggggatt tagcacgcga gacattaagg
     1141 atttccaagt gaacccaggg attggcttga gaatactggc acggccgaac gttaccggac
     1201 gactcgatat cgcctatggc cgggatgggg caaacgtctt cgtgggactg gattatccat
     1261 tctgatgctc acgctacgct gtgcaaggtg tctcggatat tcctttggtg cgagaagagc
     1321 gaacaagaca atctctctct tcattgggat cgtggtgctc gtggggcatg tcggttgtgc
     1381 cgcccctctg ttaaacgcgt cggctgctgg ggatacgaac acggtcttgc ttctcttgca
     1441 cgagggccat catgcgaatc aggcatggcc tattgtggga acgcgtccgc tcatgcttgc
     1501 cgaagcgcac gagcacgctg agaccgtccg agcgctcctc gatgcgggcg ctgacgtgaa
     1561 tgcggaagat ctgacaggct ggattgcgtt gcatgcaggc gcatttcatg gagaggtcac
     1621 tgtggtatct cttttgtcag ctcggggggc tattcaacaa ccgggccatt ggtttctgaa
     1681 gaagccgttg gagagtgcag acatattggg tcatgataat gttcttcctc tcgtgacggg
     1741 cactcatgcc cgactgtagg tcgagttgtg tatcctcaaa acgatagtcc tgttcgctag
     1801 aattgttctg ctcttccatt gagagaaatg agggggaaag tatcatgaac cattctctga
     1861 agtgattgcc gctaggtggg taccgctact gtaccagcct aacacgatag gtctttgcct
     1921 cagctggtct cgccactttc agcaccacgt cagtaccaag attcatcgca tcgtcggcgg
     1981 tagcggtgcg gcgggttgaa ttccgttcgg tgcggatcaa ggagccctgg tcgtcgtaga
     2041 agtccaccaa tgatctcgcc taattctctc aacccgctcg gtagcgggtg ccgagccaat
     2101 ccaatccaat cgtttcaacg ccggtactgc acggcgatcg ccccgcttcc ttctcttcgc
     2161 gcgctgagaa cttgcagcag acgggtctga tccagtcatt ctgccagaga catcccagag
     2221 aggagctgct cacgttcaaa acccgtccat cctggagggc tcatcctgta cgctccgttg
     2281 atgggaaagc gccctggacg ctgctttttc gggatgcagg cagcagaagc ggttgataat
     2341 gcgtgctgtg gcctcctgct gcagttatct atcaactggt ctatttattg cgaagcgcta
     2401 cagccagatc cgtctgagga agataaattc gacatgtccg tgctcaacca agaagacacg
     2461 gtgatagcgt ttgttctctc ctgtcgtaat caccatcatc caacttaaga gggataggga
     2521 acagagcact cattgtgtat tatcgccgca aacgccttgc tgataaggtt ttgctaagga
     2581 ggatgttatg gaatcgcttc gtcgccgact gttcatagtc ctggtcctca gcggggccct
     2641 cttcacgagt actgtggccg tcgaggcgca gggacgaagc gaggctgcct tgtcggcgct
     2701 ggagcggacg ccgcatgttc caaaccaggt gctggtgcag tttcgatacg ggattgcgga
     2761 agaggccaaa gaatccgtcc gaagtcgggt ccaggcacaa gtcgaagatg tggtcgtcgc
     2821 gcaagagcgg cgatccgact ttaaaggcga cctggaattg tggaatctgc cggctggcct
     2881 ggagatcgcc cgcgccgtac gagaacttga aggggactcg accgttgaat tcgcggagcc
     2941 gaactggatt tatcagcacc aagccgcatc gaacgatccg tactacacca atggctctct
     3001 ctgggggatg tacggcgacg gcaccagtcc gagtaatccc tttggaagtc aggctggtga
     3061 ggcttgggtc aacaagacgg attgttcaag cgtctatgtc gggatcattg atgaaggcat
     3121 gatgattacc catgaggatc tcgcagacaa tgtctggacg aatccctatg atcccccgga
     3181 cgggatggat aatgacggca acggctatat cgatgacatc catggctggg attttgcggg
     3241 caatgacaac tcggtatacg acggctcggg agacgatcac ggcacgcacg tgtccggaac
     3301 cactgctgct cgagggggca acggtatagg ggttgccggt gtgtgttgga acattcagtt
     3361 gatttcagcc aagttcctcg gcaggaatgg gggaacgacg gcgaacgcga tcaaggcggt
     3421 gaactacgtc acagacctca agaccagaca tggactcaag attgtggcga ccaacaactc
     3481 atggggcgga ggcggatatt cccaggggtt gtctgatgca atcaaaaacg caggcgctgc
     3541 taacattctc ttcgttgcag cagcaggtaa ttcgggggtg aatatcgata taacgccatc
     3601 ttatccagca agctatactc tcgacaatat tattgcggtc gctgccatcg acaagtcggg
     3661 aaatctggca agctggtcga attacggatc aacctcggta gacctcggtg cccctggcgt
     3721 gggcatttgg tccaccgtgc ccaacaagag aagccagtca tcctacagtt cctatagcgg
     3781 aacctcgatg gcgacgcctc atgtcacagg tgcagcggca ctgtacgcgg cggctcatcc
     3841 cactgccaca gcagcggaca tcaagcaggc gattctgtct tcgaccgtcc cgaccgcatc
     3901 gctcagcggt aagacgctga aagggggccg gctgaatatc tgttctgcct catgcccgtg
     3961 aagcagagac gggaatcagg acctgattaa tccgaacatg gattgggagt ctgtcttata
     4021 ctaagcgggc cacattctac gattggtcag ttctttcacc tgccataccg ctcggcctcg
     4081 cttcccgatt ctcgcctctc tcggcatgtt caaggcacat cgaatcggga ccgtaggtcc
     4141 ccggttccta ccacgccgag cagcaaatcg cctccagagc ctagggaggt tctctgttcc
     4201 ctcccgaaac gatccgttcc gtcacccctt cctttacaga tctcgtttca gtgatagcct
     4261 tagaacagag gtgcttttgt ggcctggcag ccagcgcctc tcagcaatct atccgcctcg
     4321 aacgaggtgc gggcatgacg ggagtcgaga cgcttctgtg gctggtcgtg gcgttcgctg
     4381 aaggaccgga ctgcctgctc caaggttcgt ttcaccggct cccggacggg ccggcgtttg
     4441 tgagggaaga tggatgtgcc gttcattcgc tagaagaagg aagctatgtg gtgttttggt
     4501 ccgagagccg ttgggtcgcg atcaagattc ctgctcgggc aaggtcactg tcctatcggt
     4561 ggggacgagc gatggcgttt gtgaacggga atagtgtgaa ggtccaacac ggcaccatct
     4621 tgaacgcatt acagcgcaga ccctcggctc gtgatcagag caaaacggac ctcatttcga
     4681 aatggagaat gcacgacgtg agagtgactc gatcgatcaa tcgccgcact atcctctaac
     4741 gcgaccggct actgagctgc ctgagttcct tcctgcttcc agtcggacat gacacaacgc
     4801 tgcgtgttga ggagtgtgaa tctgtctacg tcttctgaaa tcctggtcat gaactcttcc
     4861 acgtgacgag gcgaacgacg acgtggggtg cggcgaacat ctgctccagt gatcgatgtt
     4921 gctcggccag tgagtgaggg ccgatcacgg gaagtgctcg tggaaaacgg ttccgacacc
     4981 gttcggtcgc tcaaacattt cctaacaaag ccgtcctgcc ggcatacctg cacccagctt
     5041 gtttgacatt gctcgataac gcttcgtaag gtatttccag gtgtcagtcg aggctcgcat
     5101 cacatctccg ctaaccgatc gttccctcga ccgagagggg catcatagga ggttcgtatg
     5161 actgctttag gcgctcgcgt gctggggctg gcgggtatgc tgtgcgtgat ggcgatgggc
     5221 agcgggtgtg tggccattga agcggggcat gaaggcgtgc tggtggaaca accattcttc
     5281 ttcggtcatg gcggtgtgaa tccggtgccg gcaacaaccg gccggattaa tctgtcgccg
     5341 acaacgaaga tcatcgaagt cgatatcagg ccggttcaat acgcggaaca tttcgacatc
     5401 atttcagccg agaacgcgcc ggtgtcgttc gatgcgttta tgattgctgc cgtgatagag
     5461 ggccgctcgc ctgagctgat ctcccgcttc ggccccaatt ggtatcaaaa caacgtcaag
     5521 gaagcgttca ggacgtttgt acgggaagaa gtgcagaagt acccgttgtt ccaattaacg
     5581 accgacccga ctaccagggc aaaactgcag gatgccattg cacgagaagt acaaaccaag
     5641 ttgatcgaga agcagaacat cccggtgcgg ctcaatcgtg tcgtcgtcgg gagcattctg
     5701 ccgccgaagg gtgtggtcga gcagaccacg cagacgatta ttcaagaaca acgcaagatc
     5761 acgatggtcg agttccagaa ggcggaagag tcgcgtgaga aggccgagcg tcaacgtggg
     5821 atcgccgacc gggcctatcg ggaatccctc ggtctgaccg cgccggaatt tgtggacctg
     5881 cgccgcatcg aagtgcagaa ggaaatcgtg cagcattcgc cgtcggccct cacggtgatc
     5941 atggggttgg agcgcgtcgg catcaacatg ccgccgctcg cagcggaaaa ataggagaaa
     6001 ggcaggctgg gggaggggga tctgcttttg tccttcccct atgcagccgt tacctcacca
     6061 accacacccg ataggttttt gccgcagcgg gccttgccac tttcaagagc gcgatctcgc
     6121 cggggttcat gacgtcgtcg ccggtcgggg tgcgtctcgc cggatgacgt tcggagcgaa
     6181 taagcgagcc ctgttcgtcg tagaagtcca ccatgatctc cgctgattcc ctgagtccac
     6241 cctgataatg tgtgccgagc cagtccaatc gctttaacgc ctggtattga atcgcgatcg
     6301 ttcccctgcc ttcttctttg acgctcagga gcctgagcac acggttttgg tccagccctt
     6361 cggccgggga tgggccgctg ctcagctgcg ttcggatcac gcgcgtgctt tcttctgcct
     6421 ggagaaatcc ttttacacga cacgacaccg ttcgtccctg ctcggtctgc tccacaatct
     6481 gctggtcatg gacgtggttc gtcgccagtg tatcggccag atctcgcaac agcggcgaat
     6541 cgacgaccga cgcggttcct tcccgataga tcggcgagtt gacgacggcg aggcgaaggg
     6601 cttcccggca ggcgagggct ttggcctctg cgaccggttc ggatttatgt gcggtgtagc
     6661 ggtagtcgcc tgtgatttcg atacggccat tcggagcacc tgcggcttct gctgggaacg
     6721 ggatgagcaa cagggctgag aagaggacat cgatgtgagc cgttcgaatg agtgacatgg
     6781 ggcgcctcct cctgccagga cggacgagaa gggtcgtgat gggacgatcc gctgcctgcg
     6841 atgatgatgc taccacgccg atcgaatcct cataaaggag gggatggggg gccgttgagc
     6901 gcttccatca aggcctgcac ggtgaggggg ctgcagcctt cggaacggtt gttcacgagg
     6961 acataggccg attttccctc tgcggcggct tgcttcacga gttcgaccgt ctcctgcctc
     7021 atgcgtggct gcggctgcac gatgcgatcg taaggtttgt agcgttcaac ggctgtttca
     7081 tatttgagac cgagcggagt gaggagtcgg atgacgacgt ggggtgcggt gaaggtctgc
     7141 tccagcgatc gatgttgttc ggccagtggc ggcatgtagg accaatggtt gtagacgtga
     7201 gccacgccat gggccttaag caggtctcga tagcgggctg tgaggagcgc ggggttgcga
     7261 acttccgttg cgtactgggg gccgcggggc agctgattga ggaactggtc gagtttttcc
     7321 agaaaggtgg ccggctcggt tccccaacgt tggaactcaa agatgaaagg gccaagtttc
     7381 tcgcctaagc cttcctgggc cggccccagg acgaggtcac gaaaggcgcc tgcgtcgagg
     7441 aatcgcgggt tcggcttgcc ggctttgaca ccgtagcggg gaaggtttgc ataggcggga
     7501 atggtgatct cttcccacac cttggagcag aatcgaaagt cgtttggtac ctgttccgca
     7561 tagtgggcca gttgtgtggt agcggcggga cgatagaagg aatgatcaat tccgacggta
     7621 cggaagagcg gtgcttcaac ggcggggtgc gaggcgtatt ccgcgagcgt gtcttgactg
     7681 aaccggcttt tcgggtaggt gcggtgatag accattccct gccagccttc gtaggcccag
     7741 gaactcgtcc cgaatctgat cgtgggatac cggttcgtcg tcatgtacgt atttcgcaaa
     7801 attcattcgc aagaacaata ggatagcttg acatgttcca gtgcttcacg caaactccgg
     7861 ttcatgccag agcaaacgga acaaacgtta ctcgcgtccc tcgccgaacg ggtcggtatc
     7921 gcaacggatt atcacgacat caccggaact cgccacctca cagccgatca caccaaacgc
     7981 gcccttctca gtgccatggg gtttcaagtg gactcggcgg cggcgcttgc cgtcgccgtt
     8041 cgcgactggg acgaagcgcc ttggcgcagg ccctgtgatc ccgtccgtat tgtgcaggag
     8101 gaagaagcgg gggctccagt gctttgttgc cttgcattgg aagatggcaa agaacgatcc
     8161 gttgtcgtgg aatggcagct gcgtgacgaa gcgggcgaga tcgtgcagga tgaacgaatc
     8221 ggccccgggc tcacggcggc ggaagtgaag tttctcgacg gccatcgtca tgtacgcgtc
     8281 gaactacctg ctcccaagcc ccttcctcta ggatattatc aactggctct cagggccgac
     8341 ggtcttgtcg gagggccggt cgcgaccatg cggctcatcg tggctcctcg gcagtgttac
     8401 gtgcccccgg ggttggagtc cggccaacgt ttctggggac tggctcttca gctgtattcg
     8461 ctctcttcga gccgcagctg gggctgtggc gacttcacgg acttgaatca atttgtcgag
     8521 tgggcgggga aaagcctcgg cgcggggctg gtcggtctca accccctcca tgcgattcgt
     8581 aacagcgccc cttttcacac aagcccctat gcgccggtga accgcctcgc cttgaacgaa
     8641 ctgtatatcg atctcgaacg gttgcctgaa ttctatgcct cggatgaggc gcagaagcag
     8701 tttcgcgcgc cggagtttca ggtcaagctg caggcgatac gtgagcagcg gcgcgtcgat
     8761 tacgatgcga ttgccgccgc caagcggacc atgttggatt tggcctaccg ccaatttctc
     8821 aaagacgcct atgccggcaa cgagccgaat ctcgaacctc aaaccgcgcg cgcaaaactg
     8881 ctcgagcgat ttattcaggc cgaaggggat gcgctcgagc tctatgcgat cttccagacg
     8941 ctggaagaag aacggcgctt gatccaatcc aaggacgtga cctggcacga ctggccgaaa
     9001 caattcctgt cgcccggccc ggcggttcgt gaatacggga aacgccatcg caaacggatt
     9061 cgtttctttc agtacataca atggatcgcg acggagcaat taacggaagt ccgtcgcaat
     9121 gccgagcagg tggggatgcc ggtcgggctc tataacgatc tggcgttggg ggctgagcgc
     9181 aacggcgccg aggcctggat gtatcaatcg gtgctggctc tcgatgccga ttgcggtgca
     9241 cctccggatg cgttagcccc ggagggtcag aattgggggt tgccgccggt caatccgatt
     9301 gcgttgcgtg cgagcgggta tgaactgttt attcagctgc tgcgcaaaaa cctgcggctc
     9361 ggcggcgcca tccggttgga tcatgtgatg gcgctctgtc ggctgttctg gattcccaag
     9421 gggaagccgt cgtccgaagg cacctatgtg ctgtatccct tcgaggaact cctggcgatc
     9481 gttgcgctgg aaagtgttcg atccaaaacg ttggtgatcg gcgaggatct cggcacggtg
     9541 ccggattggg tgagggacca attggcgaag gcgcgggtat tgtcgtaccg tgtcttttat
     9601 tttgaacgtc gggacgatgg agcctttaaa ttgccgagcg agtatcctca gcaggcattg
     9661 gccgttgtgg cgacgcacga tctaccgacg ttgaacggct tctggtcggg cgaggatttg
     9721 caagttcgtg ccggcctggg cgcgttttgc gatgatgcgg ctcgccgtca ggcatgggaa
     9781 gagcgccagc gagacaaagg gcttatcctg tccgcgctcc aacaggaagg gttgttgccg
     9841 aacgagatgg gggacgaccc tgcgacagtt cctgccatga cgcaggccct ctgccgtgcg
     9901 atccatacct atttggcgcg tacgccgtcc tgggtcgtgc tggcgaattt ggaagatggc
     9961 ttggaggaac tttctcaaac caacatgccg ggcaccgtcg agagccatcc caactggagt
    10021 cggaagtatg cctgccgcat cgaggatctc atcggtgatg gacggttgcg tgaactcggt
    10081 gcctgcttgc gttcgacccg cccgatcagt taagaccctt ttgaggatcc gagggttgtc
    10141 ggtgcatttc ctcgtctcat atcttctatg ctgaaacaaa accggatcgt tctagcgatt
    10201 gc
//
LOCUS       DATIFR010000131        28193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2005067, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000131 DATIFR010000000
VERSION     DATIFR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005067"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..668
                     /locus_tag="VLD60_07510"
     CDS             360..668
                     /locus_tag="VLD60_07510"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HSB44841.1"
                     /translation="MLELRPTCEHCNKALPPPSGEARICSYECTFCAACVEHVLGNVC
                     PNCGGGFVPRPVRPSNNWRDDNFLGKDPASTTVKHRPVDLAAHELFSAAIKTIPPEQR
                     "
     gene            complement(805..1068)
                     /locus_tag="VLD60_07515"
     CDS             complement(805..1068)
                     /locus_tag="VLD60_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44842.1"
                     /translation="MGYATKSIVWRIEWCRQQSTRARAQRELEGWHAEEEGLRDALLN
                     RDHSNQYRFGPPGVFERYAMGLQDGRALLRTAAVDQEFAHSGQ"
     gene            complement(1625..1927)
                     /locus_tag="VLD60_07520"
     CDS             complement(1625..1927)
                     /locus_tag="VLD60_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSB44843.1"
                     /translation="MYDLKNLNKMKNLEAHVPEAMKAFVAFDKAAVADGAIPGKYKEL
                     MALAVAFTTQCPYCIEIHSNNARKLGTSDQEIAEAVLVAAALRAGGAITHGTHALK"
     gene            complement(1993..2814)
                     /locus_tag="VLD60_07525"
     CDS             complement(1993..2814)
                     /locus_tag="VLD60_07525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB44844.1"
                     /translation="MSTNTISPEVESVKTRLKHIWMAGDYDRFSRYLESSAREFYERL
                     QVAPRSHFLDVGCGSGQLALMAAKDGLDVTGVDIASNLIERARARAQAEGLQARFEEA
                     DAEALPFEDASFDVVASLIGAMFAPRPDLVAKELLRVCTPGGTIAMANWTPDGFIGQM
                     FKTVSTFIAPSGMPSPVLWGDETTVRERLGKGLSDLSLVRRHYTFSYPFPPSEVVELF
                     RLYYGPTNQAFASLDVGGRERLHRELEALWSSHNRGGTDCTTVLGEYLEVIGVRA"
     gene            3015..4034
                     /locus_tag="VLD60_07530"
     CDS             3015..4034
                     /locus_tag="VLD60_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSB44845.1"
                     /translation="MDVLSEVLKAVRLDGAMFYNAEFSAPWCFRSPGSRILAPYLSPD
                     ARHVIIYHLLTEGRGNAHVEGDDRTISLSAGDIVIFPHGDPHFLKNGSTVAPMDNARE
                     LERMLSQGLKVSRLGGGGEITRFICGYMACDPQLSRVFLGGLPPIMKVNIRDDASGQW
                     LENSIRYSVGHADVAVPGGKAVLAKLSEVLFIETLRRYIALLPREQTGWLAGVRDPEV
                     GKALALLHRKPADPWTIASLASEVGISRSVLAERFRRYLSETPIAYLTRWRLQLGAQM
                     LTSTSSGVAQIAAEVGYESEPSFNRAFKREFGLPPARFRSRSKSGRSRAVHHAPTNSR
                     RHAKR"
     gene            complement(4236..4505)
                     /locus_tag="VLD60_07535"
     CDS             complement(4236..4505)
                     /locus_tag="VLD60_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44846.1"
                     /translation="MKRSYIIVGLLLLGLSQTAESASNAADNQKTEGGKGTTVEDLGR
                     GLKSAAQNVEKEIPKIGSAIGETFKKITGKNSDNKPAQVTPKEKK"
     gene            complement(4760..5119)
                     /locus_tag="VLD60_07540"
     CDS             complement(4760..5119)
                     /locus_tag="VLD60_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44847.1"
                     /translation="MGPTKVVVNGSGLYDAVNGKLIKDGFASRRELEDYVNHHYLVLP
                     VVDNAGRSWQLDGKPVYCLRGVQYETVDDQKVHLARCPDCGGMGIRSDEFTVESDCIR
                     CTSCGHEFDARLEMMET"
     gene            complement(5219..5989)
                     /locus_tag="VLD60_07545"
     CDS             complement(5219..5989)
                     /locus_tag="VLD60_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB44848.1"
                     /translation="MASRTAPQKQRISVDRSIERMRQQLAELATFLGKGRPTRSLEEF
                     DLDTERLIGDLLGQTSELLHAYEYAELGEAAGLVNMTDEAPESVVMDSQRQSVLQRSR
                     VLESCVAELEARRAAEPKGAKARRQTLIGPQVAEHMTSEIQSLSQDASLREAGQRMQK
                     WKLGSLLLTDNQSYVGFITDSMLAREVVANGVDANTTPVKACMRKPVVAIAGDRPIID
                     AVRLMKEQATRHVAVTQDGQIIGVISVSNILRYYSGVV"
     gene            complement(6242..6694)
                     /locus_tag="VLD60_07550"
     CDS             complement(6242..6694)
                     /locus_tag="VLD60_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB44849.1"
                     /translation="MQVHDVMSTGIVTAKKTDSVRSVVIKMMNRNCGAIPVVEGDKRL
                     IGMVTLRDVLLPLYPNYGDYIHDNVHSRDFVEMEEGYAEALGRKVEEIMSPNPLTVAP
                     HDPVLEAASYMGLKNFRRIPVVDKGMLVGMLSIGDINRGLFFEKGMRG"
     gene            7583..7768
                     /locus_tag="VLD60_07555"
     CDS             7583..7768
                     /locus_tag="VLD60_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44850.1"
                     /translation="MRFVKVKDEERPGEAAINLDLVREAHYGGGLLRLYFERGSNTQD
                     DVTFTGENAQKIWAAMG"
     gene            complement(8026..8532)
                     /locus_tag="VLD60_07560"
     CDS             complement(8026..8532)
                     /locus_tag="VLD60_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44851.1"
                     /translation="MTRIAYRVLTSMVLAGLLLIDPHLGLSRAAEIDLLERNALVEQC
                     AERIEKWFSLITIQARELDLQHIKYIRRGDSLVVIPLKTPHGSVTGIRWRSKEQTETL
                     DLVRLLQQGKIGAVNVQPLLHDPAAMYEERGNLLVEVKGCQQKEQVQKVLGQCTVCDV
                     WEWLDIKP"
     gene            8804..10138
                     /locus_tag="VLD60_07565"
     CDS             8804..10138
                     /locus_tag="VLD60_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HSB44852.1"
                     /translation="MHSPRSHPPLRLLQLIFSTAALVSALAAAALPAQAESFVVLATY
                     DGVINPVSAEYLHDALVAAQESGAQALIIRLNTPGGLDTSMRLIIKDIIAATVPVVVY
                     VSPSSGRAASAGVFITMAAHVAAMAPGTSIGAAHPVAMGGQEMDKEMKEKVENDSVAY
                     IKSIAEQRGRNIAWAEDAVRKSLSVTEREALKLKIVDLVVEDLPALLKQLDGRTVPLQ
                     AGPTVLHTGGAAVHEFPMGLRLELLKTLSDPNIAYLLMTIGTIGLIAELYNPGAILPG
                     VVGAISLILAFYSFQSLPVNYAGVLLFLLGIVFFILEASVTSYGLLAIGGVISMLLGS
                     VMLIKTDVEFFQISWTVILPVVALAAAFSLFIVGMGVKAMRRRPVTGREEMVGLVGIV
                     KTALTPHGQLAVHGELWEATSDQPLQPGDEAQVTRVDGLRLHVIPLTKQKEA"
     gene            10140..10913
                     /locus_tag="VLD60_07570"
     CDS             10140..10913
                     /locus_tag="VLD60_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSB44853.1"
                     /translation="MMFSPLALLILAVILILMGFNVLREYERAVIFRWGRLARGLVGG
                     NGPGVVIIIPFVDKLVRVSLRTVAMDVPPQDVITRDNVSVKVNAVIYFRVVDQERAII
                     QVEDYLYATSMMSQTTLRSVLGQSQLDDLLSKREQINSDLQRIIDQQTEPWGVKVTAV
                     EVKNVDLPQEMQRAIARQAEAERERRAKVIHAEGEFEASRRLADAADVISRNPAALQL
                     RYLQTLVEIAAEKNSTTIFPIPIDTIAPFLRNFGKQAEP"
     gene            10923..11153
                     /locus_tag="VLD60_07575"
     CDS             10923..11153
                     /locus_tag="VLD60_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HSB44854.1"
                     /translation="MDQPLGISHPRDPLHGITLETIVTRLVARHGWAELGKRVPVRCF
                     RHNPTIKSSLTFLRKTPWARERIERMFIAELT"
     gene            11150..11548
                     /locus_tag="VLD60_07580"
     CDS             11150..11548
                     /locus_tag="VLD60_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HSB44855.1"
                     /translation="MTTEKKRIPTDGEPIAWTSPFAALKNVELPSAADAPSVASLPET
                     VPAKAKKHRGRVDIIRHTAHRGGKIVTVVTGFVGIGQAEKERLAKAMQKACGAGGTVK
                     EGRIEIQGDQRETVARILTEAGFRPVFAGG"
     gene            complement(11560..12744)
                     /locus_tag="VLD60_07585"
     CDS             complement(11560..12744)
                     /locus_tag="VLD60_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB44856.1"
                     /translation="MEQPSIDEALKSHLAWWGLKRFTSDADYFAWQRQQLSSAALNQL
                     NAQVERKREGDRRDEIAFYDLTAQPQILPVLYSQRYEYYGAIGSRVAARLGDAHAILD
                     FGCGVGILTTFYARQYPDKQVVGIDRSSASIAVARQKADALGLANVRFECLDVETDQL
                     AGSYDLVIATHALVQTEQDPGIPSRSWRTFERAHDRDQQAAFEQRTGIGLRLDRLAAV
                     LMNHGRVIVFEKTRQLARRIPFQRALAARGLQLIEQPEPIRYRVVEEVAEDGPLYVMQ
                     KGADLSVDWDESPEPDREVLFDRSAGQRNSSDREAPLYENHGHSAQCVWERLKDRTVT
                     KETTRQEPDGRQMHVELGTSEDVSYLYCANTFDQRQVVIVDPPRTAMIETYYQEIADG
                     IP"
     gene            complement(12751..14130)
                     /locus_tag="VLD60_07590"
     CDS             complement(12751..14130)
                     /locus_tag="VLD60_07590"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSB44857.1"
                     /translation="MTGVAMPEVPVKLYLDKLLKECRKNVAPMALLSGPVKDKALRAM
                     ADRLEADEEAILAENEKDVDAVGKFYSSDVNKDRMKAAVARLRMTADDIKEMVDHLRV
                     IADQPDPVGAVTSRWERPDGLQVSRVRVPIGVIGIISEMKPLITVESIGLCLKSGNVA
                     VFRGAPEWSLTHQAIGKGLSEAAQQHDVPAGAWTIVERSEKEAAIELIRSGKVLDAII
                     PRGGAGLRKTVMEGARMPVLCHDGGLTHLYVDADTDMPMAQNIAINSKAQLAGAANAL
                     DTLLVQQVIGRQFLPPLINRLLDEFKIEVRGCPKTVALMGQMAMTGHTSIKPATEEDW
                     RTQFQAPVLAVKMVASMDEALAHIAEHGPCLTAVIATTNYNSAMRFTREVDASAVFVN
                     ASSRLNAGDSYGSGADIGLSNARLHAKGPIGLEQLTCEKYVAFGSGQLRLPHPVPEAY
                     FDAIMLKRP"
     gene            complement(14225..15496)
                     /locus_tag="VLD60_07595"
     CDS             complement(14225..15496)
                     /locus_tag="VLD60_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44858.1"
                     /translation="MSSVRGSIQSITGAEPDELLPVCWSFLYFFCLLCGYYILRPVRD
                     EMAIEGGVQNLPWMMTATFLSLILVTPLFGYLSARVPRYRLLVTVYAFFGINLLGFFL
                     LLINHVAPHWVARVFFVWLSVFNLFVVSVFWSFMADLFTPTQGARLFAVIAAGGSTGA
                     MVGPLLTTALTYVVPVPLLMLISAGFLAVCGLCIARLDRWSRYRATPQAGLQGEPIGG
                     SMWAGIRLALRSPYLLGICLYLFLLTTTATFLYLEQTRLVGEQIQSPEGRTRLFALID
                     LVVNVVTFLTQVFITSQMISRFGVASALIILPLASMAGFGMIGFIPVLVMFVLFTIVR
                     RICEYAIAKPAREILFTVVSREEKYKAKNFIDTAVSRGGDAATGWVVQGVKAFGITAS
                     QIAWLLVPLALFWGWVGWRLAREEEERRIQK"
     gene            15583..16617
                     /locus_tag="VLD60_07600"
     CDS             15583..16617
                     /locus_tag="VLD60_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HSB44859.1"
                     /translation="MRIIDLRSDTVTKPTEAMRDAMARAEVGDDVYGEDPTVNRLQEM
                     AAGLLGKKAALFVPSGTMANQLAIRVHTQPGQEVIAESKAHIVRYEQGAAGALAGVQL
                     HWVVGDRGIIGPEQVAAAIRPKDPYSIQTALICLENTHNSGGGTIYPLATIERIRAVA
                     SAHGIPMHLDGARLFNAVAATTLPPAAYATHFETVSLCLSKGLGAPAGSLLLTNDPTL
                     LEKARRFRRMYGGAMRQAGILAAAGIYALEHHVGRLKEDHDNAKRLARKLQQIPAITI
                     NPQHVDTNIVIFDVIGHRLAPAAIVAALKQEGLLINAVGGTSFRAVTHLDVSTTAIDR
                     ACDIFARVLG"
     gene            complement(16685..16867)
                     /locus_tag="VLD60_07605"
     CDS             complement(16685..16867)
                     /locus_tag="VLD60_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44860.1"
                     /translation="MQPLLERLICVNHDFQFPVTQSPDKARLFRREGNLHPLPIQAER
                     VGQLEDTTDVRERDNL"
     gene            16912..17697
                     /locus_tag="VLD60_07610"
     CDS             16912..17697
                     /locus_tag="VLD60_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44861.1"
                     /translation="MDTERAPTLPSREELQAELVPLPEPPEPPEPLNEPGFEDTVAKA
                     LRHVRGRRGGDLVSVLLVGSGVRRAVTAHSDVDLIVLVKGHADGHEIVRIADRMADIR
                     YWGHKEVEEDLTFSPRLPSLLRKARILHDHDGIGAKLIERANQRFRQGPPPAGINEQI
                     RLKAECFHWLGKAQDLADKPATAQYLLTIFFDDFVNAFFRLRGLWVTAPVDVPRFIAS
                     RDVDLGDLAGRFLSAATLPERLNCGRDLANLLFKDVPNPARID"
     gene            17952..19487
                     /gene="zwf"
                     /locus_tag="VLD60_07615"
     CDS             17952..19487
                     /gene="zwf"
                     /locus_tag="VLD60_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSB44862.1"
                     /translation="MPPISRIDIKPTQEALAPVEPCTLVIFGGSGDLARRRLIPALYN
                     LLLDGLLPDNFAVLGLGRKTMTDEEFRATVREGVLAHSRQSLVEDRWKTFADHLFYLA
                     GENDNPKTYEALKARADAIEGMLGLPGNRIFYLSIPPSSFTAVCEGLEQSGLAVRTTR
                     PYARVIVEKPVGRDLVSAQHINAVTGRVFDESQIFRIDHYLGKETVQNLMVVRFANSI
                     FEPIWNHKYIDHVQITVSEAEGVGSRAAYYEEAGALRDMVQNHLLQLLCLVAMEPPYS
                     LDPDVVRNAKMEVLRCLRPISGKDVDTYTVRAQYAAGSVHDQPVPGYRRENGVKPDST
                     TETYVALKCFVENWRWSGVPFYLRTGKAMPHRASEVAVQFKDIPQILFNANAQQPQPP
                     NVLALRIQPEEGLSLRIVSRVPGTRAQTHPVEMDFQYSDVFGRPSPEAYERLLLDVMT
                     GDASRFMRRDAVEASWAWITKILEGWQQQGARWLPEYSAGSWGPVEADRLIQQDGRIW
                     RTL"
     gene            complement(19497..19811)
                     /locus_tag="VLD60_07620"
     CDS             complement(19497..19811)
                     /locus_tag="VLD60_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSB44863.1"
                     /translation="MISEVTDRTFDRLVEDATTPVLIEFWKPGCGTCRALMRQLEQLE
                     ARVAGRVMILTMNVDENFQIPAELEIRSLPVLALYRRGQFERFIGGIGTKDEILKQLA
                     LS"
     gene            complement(19801..20439)
                     /locus_tag="VLD60_07625"
     CDS             complement(19801..20439)
                     /locus_tag="VLD60_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSB44864.1"
                     /translation="MIICFGDSLTAGFQSPTPENPAGQETPYGQFLQERLGASVEIRV
                     SGICGELTGEMAMRFRRDVLQQQPSHVVLLGGTNDLGWNAQPADIMRNLVKMYELARA
                     SKIIPIPVTVPSIRVEATGGGPDAEAWIEQHLVRRVELNGLIREYAVSHNVPWIDLFA
                     ATAEPSTRRLAAQYSNDGLHLTTSGYRLLARLLYDGVFARSVSSAGEYGHDL"
     gene            20704..22413
                     /locus_tag="VLD60_07630"
     CDS             20704..22413
                     /locus_tag="VLD60_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB44865.1"
                     /translation="MAVKQNADPELTSVTQLLKSLDRASKNVRTFGQRNPVAQKFFAQ
                     YYAELTAHLTQFNSLTFVVQRDGLFLKEELVYGSQTGDANENFAFKLYSDGIREITFH
                     QDITDEDVLFFFDALWDTVGDAGSEDDDIVTRLWAKNLPTLTIVTADEVMKLSELDDV
                     LAPQGKAPMEGSLREIIAESKAREVKDASGTKQQKRRFSSGVTGYEVSEEELAALASE
                     ITVESSRDNVLHILDMLTAILSSEKSPDLLDKLFDIYGAILQSLIQRGHWSAMEHVLG
                     LLSEAEAVRPDLTEEHKKHIQQLFEHLGSPETVDLIGTYLNTTDIPRIDGLSTIFLMM
                     KPTAVPALCTLLGNLEQQAHQALVINALVEQAKDTPEVVAKHLTDRRPTFVRNLLGII
                     TRWNNPRLADNVEKILRYPDPLIRREAIRTLATLRPSGTATKLIPLMNDSDEGVRLAT
                     LKSLLTGNYSAPFSSWESIVNAEAFGDRPPAERRNIFHAMRMTCGDEAVPYWTTLMSE
                     WGWTNKKKREDLALMAVDALGKLGTPAAQTALDTGTKKGTSAVKQACATALAGVSKHP
                     RTA"
     gene            22441..23949
                     /locus_tag="VLD60_07635"
     CDS             22441..23949
                     /locus_tag="VLD60_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSB44866.1"
                     /translation="MAEPLRTSDTASSPVSAGQPILTHEKSLTNKVAKGSEAGDIYDQ
                     QLLMLGAQLVTQISILIKMSRLHGRTNAALDNPVEAALTVIKTLAHDEPASIRLQNDF
                     LFLGQRHLKVTASQMPVFSIVIDALNNWKIGGISFSKETESRDFREFVFLLASFDPTT
                     SSIKTLRTALEQNSVKGIQLEERRVLRGKATAKTRYANAAAAVGKLVQDSREKGTIGF
                     KQAKRAIQSIVDLMMNDEASLLGLTTLRCHDQYTHNHSVNVSLLSMALGNRAGYPKVE
                     LADLGLAALFHDLGKANIPMEVLNKPGEFTDEEWQMMRNHPTEGALSLTKMRGITNLP
                     GRMVSASFEHHMNLDFSGYPKLNVSWKLSMTGRILMIADCYDAMTSSRVYRREPMSPA
                     KVLGFMFGKAGKAFDATLMKLFMTCVGIIPIGSLVMLDTNELAVVLKPAADPTDAEHP
                     YVKVITDANGTAIENGPELDLHEKDETGEYRFNIIRLIDNTEHKFDTSRYFV"
     gene            24047..24610
                     /locus_tag="VLD60_07640"
     CDS             24047..24610
                     /locus_tag="VLD60_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSB44867.1"
                     /translation="MFTVKTLWGRTLFGSVATGALLVGLASGATAADTFKMGVIDPQA
                     VLEKSKAGRKALDGLKEYVSTRQKLLSRDEEELRNTEKTLKESGSKLSDAEKKEKETQ
                     FRAKIQDYQKRAQEFNQELQGKQKELVDDYMKRIASATQTVAEKGGFSIVVDKGSEQT
                     VKIVIYNKDTIDLTDQVIKEFDRVNSK"
     gene            complement(24653..25021)
                     /locus_tag="VLD60_07645"
     CDS             complement(24653..25021)
                     /locus_tag="VLD60_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSB44868.1"
                     /translation="MKHNAGFLKLVDAARARVKECTVREAKARLAREKGVHFVDVRED
                     HEYAKEHARGARHIGKGIIERDIETVIPDKQAPIILYCGGGYRSALAADALQQMGYTN
                     VCSMDGGIKAWRDAGYPMEQ"
     gene            complement(25042..26055)
                     /locus_tag="VLD60_07650"
     CDS             complement(25042..26055)
                     /locus_tag="VLD60_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HSB44869.1"
                     /translation="MRAVSNPPNPFELQYRELLEPASPARLELYEDAAKEILSRNESP
                     DLPFRWSLNPYRGCFHGCAYCYARPTHEYWGFGSGTDFESKIIMKRDAPALLRKAFEK
                     PSWRGELIVFSGNTDCYQPVEASLELTRACLEVCAEYRNPVGIITKGILVLRDLELLK
                     RLQQEVWVRVYFSIPFADDEVARLVEPHAPSSGRRFDAMARLSQAGIPTGISISPLIP
                     GLNDEDIPELLERAAQAGAGESMATLLRLSGPVEDVFLERMAQAFPDRLAKITHRIRE
                     VRRGALTDSAFFSRHQGSGPYWTMIEQLYDVARKKAGFRILEEEAVPCTFRQPHAEQA
                     TLF"
     gene            26169..27455
                     /gene="serS"
                     /locus_tag="VLD60_07655"
     CDS             26169..27455
                     /gene="serS"
                     /locus_tag="VLD60_07655"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSB44870.1"
                     /translation="MYDLRNLRDNLDAIRNQLGPRGADVQWEDLRKLIEERRGLTSQV
                     ETLRHDLKKGSDEVARLKREKQPAEEAVAAMKAVGDRIAQCETGLRGVEEKLQDLALR
                     IPNLPHPTVPVGRSTEQNVEVRRWGTPPTLGHPAKTHWDIGENLGILDFDRAAKIAGA
                     RFAVLTGAGARLERALINFMLDLHTTEHGYREVLPPLLVNRSAMTGTGQLPKFEEDLF
                     RLRDEDYFLIPTAEVPVTNLHREEILDESVLPIRYTAFTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFTKPDQSYGELERLTGHAETILQRLGLHYRVMALCTGDLGFSAAKTY
                     DLEVWLPSQNQYREISSCSNFEGFQARRAGIRYKGTGGKKDGKTDFVHTLNGSGLAVG
                     RTLVALLENYQQADGSVLVPEALHGYMGGLEQIEKE"
     gene            27594..27683
                     /locus_tag="VLD60_07660"
     tRNA            27594..27683
                     /locus_tag="VLD60_07660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27628..27630,aa:Ser,seq:tga)
     gene            complement(27870..>28193)
                     /locus_tag="VLD60_07665"
     CDS             complement(27870..>28193)
                     /locus_tag="VLD60_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HSB44871.1"
                     /translation="ALAQSDTQALSIAVSAALTITTTSLPNATVGKSYSTTLQRSGGV
                     SPFFWSVTPALPAGLSLNTSTGKITGTPSAGTAGTYSLTFTVQDSSTPTKQTAFKVLS
                     LTINP"
BASE COUNT         6112 a         8388 c         7764 g         5929 t
ORIGIN      
        1 gtggaagggt tcgacgtgca tgtgccgaag ggatatatat attttgccat ggcgttttcc
       61 gtcaccgtgg agatgctcaa tattcgcatg cgcagaaaac gggcggcacc ggtcaaactg
      121 catagtaaat actcgggaga ccaccgaccg gaagcgacct agcgtcacat cctctacctc
      181 ttcagttctt gcggatgttc atgaagagtg agggatgggt cttgcaatct aacaggcgcg
      241 gcaatccggc ggtttattcc cgttccctcg cctccagagt gatgggtgcg cttcacattt
      301 tgcagtagac ttgccgaaga actccgctcc gtgagccgct ctacagaaga ggagccgcca
      361 tgttggaact caggccgact tgtgaacact gcaacaaagc cttgccgccc ccctcaggtg
      421 aggcgcgcat ctgctcctat gaatgtacat tctgcgccgc ttgcgtcgag catgtgttgg
      481 gtaacgtctg tcccaactgt ggtggcgggt tcgttcccag acccgtcaga ccgtcgaaca
      541 actggcgtga tgacaacttc ctaggcaagg atccggcgag cacaacggtc aaacacagac
      601 cggttgatct cgcggctcat gagttgttct cggccgctat caaaaccatc ccacctgaac
      661 aacggtagtt cgcgaacgtg ggaaatcaga acgtcggcca agagaagatg gagggtgagg
      721 tttgacaatt cgaactcttc gcgagacacg agagaggcac agcctcgagg aactcctggt
      781 ctagacttgg atcaaccttg taccctattg gccggagtgt gcgaactctt ggtctaccgc
      841 tgctgtgcga agcagcgctc gcccatcctg gagccccatg gcataccgct caaacacgcc
      901 gggcggacca aaccggtact ggttcgagtg atctcgattg agaagggcat cccggagccc
      961 ctcctcttcg gcgtgccatc cttcaagctc acgctgcgca cgcgcccgtg tgctctgctg
     1021 tcggcaccat tcaatccgcc atacgatgga tttggtcgcg tatcccatgg ctttcctccc
     1081 ttgctgggat gtccgagtgc tgactatgct gcaagtctgt ctcgtcgctc cttgttgaat
     1141 tgctgaatcg caaagtaaat gccagttaac taaaacatca tactctctga cgctgtttac
     1201 ttgctgagtg tcgcttttta tatatgttcc aaccagttac ggtacctcat tagctcgtca
     1261 tcgggatcat aatcaattct tcgattcgta cagatctgaa gaatctgaag gactaagtat
     1321 ccacctcagg acaaactgtc tcgctccgta ggactaatca ttaagggctc accccctgca
     1381 atactgcatc ccgccgcgtg tagcggagag cttgttcgtc agggcgaggc tgcaagacta
     1441 gcgggacgag cgagacgggc atgacaaggt gcaaacccac gctctttccc tctcgccttt
     1501 cgcgcatctc tcgcctgtct cgcgatttcc gctcaagcct tcaccccata cttcaataga
     1561 ccaggctggc ctaacagggt tgaatatggc ggtttctcag gtcattcaaa aataccgttc
     1621 agattcactt cagggcgtgt gtgccgtggg tgatcgcccc acccgcgcgc aacgccgcag
     1681 caactaatac ggcttcggcg atctcctgat ctgaagtgcc tagctttcgc gcgttattcg
     1741 agtggatctc gatgcaatag gggcactgcg tcgtgaacgc gacggcgagg gccatcaatt
     1801 ctttatactt gcccggaatt gctccatcag ccaccgcagc cttgtcgaag gccacaaacg
     1861 ctttcatcgc ctcgggcaca tgagcctcta gattcttcat cttgtttaga ttctttagat
     1921 cgtacatggc cacctcctga tgatattcgg ttgctcaatg acatttgcca tgtcattccc
     1981 ttcctcgaca tttcacgctc gaacgccaat cacttccaga tattcaccga ggaccgtggt
     2041 gcaatcggtc ccccctcgat tgtgagagga ccataacgct tcgagttccc ggtgaagacg
     2101 ctctctgccg ccgacgtcaa gagacgcaaa tgcctgattg gtcggtccgt agtagaggcg
     2161 gaataactcc actacctccg agggcggaaa gggataactg aatgtgtagt ggcgtctgac
     2221 gagactcaga tccgacagcc ccttgccgag ccgttcccgg actgtagttt catcccccca
     2281 gagaaccggc gagggcatcc cggacggggc gataaatgtc gagacggtct tgaacatctg
     2341 cccgatgaag ccatctggag tccaattggc catggcaatg gttccgcccg gtgtgcacac
     2401 gcgcagcagt tccttcgcaa caagatccgg ccgcggggca aacatggctc cgatcaggct
     2461 cgccaccacg tcaaagctcg catcctcaaa gggcagcgcc tctgcatcgg cttcctcaaa
     2521 gcgcgcttgt aacccctccg cttgtgcccg cgctcgtgcc cgttctatca ggttgcttgc
     2581 gatgtccacg ccggtgacat ccagaccatc cttcgcggcc atcagggcca gttggccgga
     2641 gccacagcct acgtccagga agtgacttcg tggagcgact tgtaagcgct cgtagaactc
     2701 ccgcgcactg ctttcgaggt aacgcgagaa tcgatcgtag tcaccggcca tccagatatg
     2761 cttgagacga gtcttcacac tctcgacttc aggtgatatg gtgttggtcg acattctgcc
     2821 ctccttgtat gtgtgatcgg gtcgacgacg gttcggcatc gccgctgcaa cgatgtgtcg
     2881 cactataggg cgattatgtg tggctgtctt atccgggagt ctggttctct tgactgggtc
     2941 gtgctaggct gcgctttact tgcttcggcg tccggtacac tttgccgaaa gtccgtgcca
     3001 gcagtggagg ccgcatggat gtcctgtccg aagtcctgaa ggccgtcagg ctcgacggag
     3061 ccatgttcta caatgctgag ttttcagcgc cctggtgctt tcgctcgccc ggctcccgca
     3121 tcctggctcc gtatctatcg ccggacgcca ggcacgtcat catctatcac ctcctgaccg
     3181 agggccgcgg caatgcccat gtcgagggag acgaccggac gatttctctc agcgcagggg
     3241 atatcgtcat ctttccgcac ggcgacccac acttcttgaa aaacggctcg accgtcgccc
     3301 cgatggacaa tgctcgagag ttggaacgaa tgttatctca aggcctcaag gtctcccgcc
     3361 tgggtggagg cggagagatc accagattta tctgcgggta tatggcctgc gacccgcaac
     3421 tcagccgggt cttccttggc gggctgccac ccattatgaa agtcaacatc cgcgacgacg
     3481 cgtcagggca atggctggaa aactccatcc gctattcggt gggtcacgcg gatgtcgccg
     3541 tgcccggggg taaggccgtg ctcgccaagt tgtctgaagt cttgttcatt gaaactctgc
     3601 gacgttacat cgcgctgctc ccgcgggaac aaaccgggtg gctggccggc gtccgcgatc
     3661 cggaagtcgg gaaagccttg gccctgctgc atcgcaagcc ggccgatcct tggacgattg
     3721 cctcgctcgc aagcgaggta ggaatttcac gttccgtact ggccgagcgt ttccggcgtt
     3781 atctctcgga aacccccatt gcttatctca cacgctggcg gcttcagctc ggcgcacaga
     3841 tgctcacatc cacgagcagt ggcgtggcgc agatcgctgc cgaggttggc tatgagtcgg
     3901 aaccgtcctt taaccgcgcg ttcaaacgtg agttcggcct gccgccggcc cgatttcgca
     3961 gtcgatcgaa atcaggccgc agcagagcgg tccaccacgc accgacgaac agtcgccgtc
     4021 atgcaaaacg atgagcgagg cggcattgct gatggagggc atcggatgcc ctcctcgttg
     4081 tggctgggat cttgaccaaa acccaggaca ggttgcatac aagaacattc ataagaagct
     4141 ttggcgtgtc tggaaaagaa gcgggcgcca ggaaatattt cgcggcgcgt ggcgggtccg
     4201 gtggcggcgt aattcgggtc acatttctcg gtgggctatt tcttctcttt gggggtgact
     4261 tgagcgggct tattgtctga gttcttccca gtgatttttt tgaacgtctc tccgatggcg
     4321 gagccgatct tggggatttc tttttccacg ttttgcgccg cactcttcag gccgcgcccg
     4381 aggtcttcca ccgtcgtccc tttgcctcct tcggttttct gattgtcggc ggcatttgaa
     4441 gcggattcag ccgtctgact cagtcctaac agtagcagcc ccacgatgat gtatgatctc
     4501 ttcatcgttc acctccgcac gtaaaactat agcatgttca aagaggcgag gtcggatcta
     4561 gcggtctaca tttcgcggcg tgtggcagga atttggttca tgtaattgtt ttgatttgtg
     4621 acggtgggag ccacacaatc taaacgcgat ggccatctcg ggcgactgga agaggtgtcg
     4681 gtcaccattt cagagcgagc gagagccctt agggaccacg cgaggcgtga tgtgagacca
     4741 gtgacccgac aagcctcttc tacgtttcca tcatttccaa ccgggcatcg aactcatgcc
     4801 cgcaggacgt gcagcggatg cagtccgact ccacggtaaa ctcatcactt cgtatcccca
     4861 tgccgccgca atccggacag cgggcaagat ggaccttttg atcgtcaacg gtctcgtatt
     4921 gcacaccgcg aagacagtac accggcttcc cgtcgagctg ccaggaccgg ccggcattgt
     4981 cgacgaccgg caaaaccaga taatgatggt tcacgtaatc ttcgagttcg cgccgactgg
     5041 caaacccgtc cttgatcagc ttgccgttca ccgcgtcgta cagcccgctg ccgttcacga
     5101 ccactttagt cggtcccatc ggttcacctc cctgcgcgaa gatacggaaa acgttaacac
     5161 gaacaagagc gctgccccgc agttgaatcg cgcttcacac gcgacgattc cggtgtcgtt
     5221 agacgacgcc ggaatagtat cgcagaatat tcgaaaccga gatgacgccg ataatctgtc
     5281 cgtcttgcgt tacggcgacg tgcctggtgg cctgctcctt catgaggcgg acggcatcga
     5341 tgatgggacg atcgccggca atggcgacca ctggtttacg catacacgcc ttgaccggcg
     5401 tcgtgttcgc atcgacgccg ttggcgacca cttcgcgcgc gagcatcgaa tctgtgatga
     5461 agcctacgta cgactgattg tcggtcagga ggagcgagcc cagtttccat ttctgcattc
     5521 tctgccctgc ctcccgcagg ctggcatcct gggagaggct ctggatctct gacgtcatat
     5581 gttccgcgac ctgcggcccg atcagggtct gccgcctggc cttggcccct ttcggttcgg
     5641 cagcccggcg cgcctccagt tcagcgacgc aactttccaa cacccggctc cgctggagca
     5701 cgctttgccg ctggctgtcc atcacgacgc tttctggcgc ctcatccgtc atgttcacaa
     5761 gaccagccgc ttcgcccagc tccgcatatt cataggcatg gaggagctcg gaggtctggc
     5821 cgaggagatc gccgatcaat cgctccgtat ccagatcgaa ctcttccaaa ctgcgggtcg
     5881 gcctgccttt gcccaggaag gtggccaatt cagccagctg ttggcgcatg cgttcgatgc
     5941 tgcggtcgac ggaaatccgt tgtttctggg gagcggtgcg cgaggccata gcacctcctc
     6001 tatttctatt attgatacgg cgggagtggc cgaatcatac ctccatcagg atcagcgagg
     6061 caagcgcccg atccctggca tgatcgcgca cataaccgaa tcgccgttcc ctgattcctc
     6121 cttcgtgaag cctcgttcgt cgcttgccga gcacgcttcc cgatcttgcc atcgcgcatg
     6181 caagatcggc tgaacgggat agatgctcct gcacgacaca tgcatcagcc cgatgctgat
     6241 ctcagcctcg catccccttc tcgaagaaca agccacggtt gatgtcgccg atgctgagca
     6301 tgccgaccag cataccctta tcgacgacag ggatacgccg gaagttcttg agtcccatgt
     6361 acgaggccgc ttccaacacg ggatcgtgag gtgccaccgt gaggggattt ggactcatga
     6421 tctcttcgac tttccggccg agtgcctcgg cgtacccctc ttccatctcg acaaaatccc
     6481 ggctgtgtac gttgtcgtga atgtagtcgc cataattggg atagagcggc agcaggacgt
     6541 cgcgaagcgt caccatcccg ataagccgct tgtccccttc cactacggga atggccccgc
     6601 agttgcgatt catcattttg attacaaccg accgaaccga atccgtcttt ttagcagtga
     6661 cgataccggt tgacatgaca tcgtggactt gcatggccac ctccttctcg taatgcgatg
     6721 agaagtccgg ctctcgaccc cgtgcgtgct ccctcaacgc cgcaacgacc acaccgggtc
     6781 gcctgccttc aaagatggct aatcgatcaa gaaggataca gcacgggcga ccttacacta
     6841 aggttgaagg tcgcccgtgt gtggcgctta ggacgccgac tgactttgtc gtgtaatgct
     6901 gatccggaag acccgttcga ggagtgggtt caaaggcggc tcgtcagggg agaagagact
     6961 cgatcccagg cgcttgtgtt cctcacacca gcctagactc tccagaatgg ctcatcggta
     7021 tttgttattc cctgacgcgg gaaggcaaca gccgatggag aaccggctag cgtgactgac
     7081 gagaagcccg cccgtaactc ggcgggccgt ggtggtccgg tggacttagg tcttccatct
     7141 ccacctcctc cttgcttcgg agtgacctgg gggattatgc cgacagcctc gccctgcgcc
     7201 atccccctct tcatgaacga ctgcacgaac ggtttcatcc ttactcctct tcaacaaact
     7261 ctgtcaacgg gtgacattct gattttttta gacaggtgcg agcataaccc gtgccataca
     7321 aaacagactc aggacgaatt gaagaaggga caagaggacg ggcgctacgc gtggaggaag
     7381 ttgatctatc tagcttttgt ggtctttctc gtttgccccg ctcacgcaac gcagaccgtc
     7441 ccgatctgca accgcgacat gccattccca ccttgactca tgcttcctag acgcataatt
     7501 taggctgaac ttttcaccga agcgggactc attggtctca tgtgaaaaga aatcgtcaca
     7561 tctacaaaag gaggcacgag taatgcggtt cgtcaaagtg aaagatgaag aacgcccggg
     7621 tgaggctgcg atcaatctgg atctcgtccg agaagctcac tacggagggg ggctgctgcg
     7681 cttgtatttc gagcgcggct ccaacacaca ggacgacgtc acgtttacag gtgagaacgc
     7741 acaaaaaatc tgggcagcga tgggatagta aaatttggtg gagaaatggg gtggaggtct
     7801 tattcaggag aagaaaaaga catttcgtag tttggatacg cgatgaccgc cagaacctcc
     7861 acccattttt actggaacgg attgtggcaa taatgctcaa cgcagggaaa caaaaatgaa
     7921 tgaatgggtc tggccagtca acatgtcacg gcgcgtgaca gggatctggt tcatctcgtt
     7981 tattcctgtg ttttgtttct tgcgctcgac tcgttccgca gcacattatg gtttgatatc
     8041 cagccattcc catacatcgc atactgtgca ttggccgagg actttctgaa cttgctcctt
     8101 ttgttggcac cccttcactt ctaccagcaa atttcctcgc tcctcgtaca ttgcggccgg
     8161 atcatgaagt aagggttgaa cattcacggc gccgatcttt ccttgttgaa gcagccgaac
     8221 aaggtcgagt gtttcggtct gctcttttga acgccaacga attccggtca cacttccatg
     8281 tggtgtcttc agagggatga caacgaggga gtcccctcgc cggatgtatt tgatgtgttg
     8341 caaatcaagc tcccgggctt gaatggtgat aagtgagaac cacttttcaa tccgctcggc
     8401 acattgctcg acgagcgcat ttctctccaa gagatcgatc tcggccgccc tggacaatcc
     8461 gagatgcgga tcaatcagaa ggagacctgc caggaccatg ctggtgagga ctcgatatgc
     8521 gattcgtgtc atatgaattc tcctgattac tggatacgtg atcgtcatgt gtcgataaac
     8581 atgacggcgt cagccttcac ggcgtcgttg agggaccggc tgcaagcata cgccgaaaaa
     8641 ccttggtaaa tcgagatgtg ccggacacgc tttcgctatt caccacttgg tcattatgca
     8701 atcccaccct tccctttcct tgacagggta tcgagtgact cgtatgctgc atcgagcggt
     8761 ttcacgtcct tcttcctcag ccttttccgg gagggacacc tcgatgcatt ctccgagaag
     8821 tcatcccccg ctgcggctcc ttcaactgat tttttctacc gctgcactcg tcagcgcatt
     8881 ggctgcggcg gcgcttcctg cgcaagccga gtcgttcgtg gtactcgcca cctacgatgg
     8941 ggtgatcaat ccggtctccg cagaatacct gcacgatgcg ctcgttgccg cgcaagagag
     9001 cggggctcag gccttgatca tccggctcaa tacgcccggt ggtctcgaca cgtcgatgcg
     9061 gctcatcatt aaagacatca tcgccgccac cgttccggtg gtcgtctacg tgtcaccgtc
     9121 cagcggacga gccgcctctg ccggtgtgtt tattaccatg gccgcgcatg ttgcggcgat
     9181 ggctcccggc acaagcatcg gagccgctca tcctgtcgcc atgggaggac aggagatgga
     9241 caaggagatg aaggagaagg tcgagaacga ttccgtggcc tacatcaaat cgatcgccga
     9301 gcagcggggt cggaacatcg cctgggcgga agacgccgtc cggaagagcc tgtccgtgac
     9361 ggaacgagaa gccttgaagc tcaagatcgt cgatctggtg gtggaggacc tgccggcgtt
     9421 gctgaagcaa ctggatggcc gcaccgtccc tctgcaggcc ggcccgacgg tcttgcacac
     9481 agggggcgcg gctgtgcatg aattcccgat gggacttcgc ctcgaactct tgaagacgct
     9541 cagtgacccg aacatcgcct atctgctgat gacgatcggc acgatcgggc tgatcgcgga
     9601 attgtataac cccggggcca ttctccctgg cgttgtcggg gccatcagct tgatcctggc
     9661 cttctactcc ttccagtcgc tgccggtgaa ctatgccggc gtcctgttgt tcctgctcgg
     9721 gatcgtcttc ttcattctgg aagcctccgt gaccagctac ggcttactgg ccatcggcgg
     9781 ggtgatctcg atgctcctcg gatcggtcat gctgatcaag accgatgtgg agttctttca
     9841 gatttcctgg acggtcatcc tgccggttgt cgcactggcc gccgcattct cgctgtttat
     9901 cgtcgggatg ggggtgaaag caatgcggcg aaggccggtg accggacggg aggagatggt
     9961 cggtctggtt ggcatcgtca aaacggcgct gactccgcac ggacaactag ctgtccacgg
    10021 cgaactctgg gaggccacca gtgatcagcc cctccaacca ggcgacgaag cccaagtcac
    10081 acgcgtggat ggcttacggt tgcacgtgat ccctctcacc aaacagaaag aggcctaata
    10141 tgatgttcag tcccttggcg cttctgatcc tggccgtgat cctgatcctc atggggttca
    10201 atgtccttcg agagtacgaa cgtgccgtga tcttccgatg ggggcggctg gcccgtggac
    10261 ttgtcggggg gaatggaccc ggtgtcgtga tcatcatccc gtttgtcgac aagctggttc
    10321 gcgtcagctt gcggaccgtg gcgatggacg tgccgcccca ggacgtcatt accagggaca
    10381 acgtcagcgt caaggtgaac gcagtcatct actttcgggt tgtggaccag gagcgagcca
    10441 tcatccaagt cgaagattat ctctacgcca catcgatgat gtcgcagacg acgcttcgca
    10501 gcgtactggg gcagagccag ctcgacgact tgctgtcgaa acgggaacag atcaactcgg
    10561 acctccaacg gatcatcgat caacaaacag agccctgggg tgtcaaagtc actgccgtgg
    10621 aagtgaagaa cgtggacctg ccgcaagaaa tgcagcgggc tatcgcgcgc caggcggaag
    10681 cggagaggga acgccgtgcg aaggtcattc acgccgaagg cgaattcgaa gcttcgcgac
    10741 gcctggccga tgccgccgat gtgatcagcc gaaacccggc tgccttgcag cttcgctacc
    10801 tgcagaccct ggtggagatt gctgccgaga agaactcgac gaccattttc ccgatcccca
    10861 tcgataccat tgccccgttc ctcaggaact ttggcaagca agcggaacca tagacctggc
    10921 atatggacca gccactaggc atctcacatc cccgcgatcc actgcacggc attacgctgg
    10981 aaaccatcgt cacgcgactg gtagccagac atggttgggc tgaactggga aagcgcgtcc
    11041 ccgtgcgctg tttccgtcac aaccccacga tcaagtccag cctcaccttc ttgcgaaaaa
    11101 ctccctgggc gcgcgagcgg atcgaacgga tgttcatcgc ggagctgacg tgacgacgga
    11161 aaagaaacgc attcccacgg atggtgagcc gatcgcctgg acgagtccgt ttgccgcgct
    11221 caagaacgtg gaacttccgt ccgctgctga tgcgccctct gtcgctagcc taccagagac
    11281 ggtaccggcc aaagccaaga agcatcgtgg acgcgtggat atcattcgcc acaccgcgca
    11341 ccgcggcggg aaaatagtga cggtcgtgac aggtttcgtc ggcatcggtc aggctgaaaa
    11401 agaacgcctg gccaaagcga tgcagaaagc ctgcggagca ggagggacgg tgaaggaggg
    11461 tcgcatcgaa attcaggggg accagcgcga gaccgttgcc cgcatcctga ccgaagccgg
    11521 ctttcgacct gtatttgccg ggggttagcg cgctccgcgc tacgggatcc cgtcggcaat
    11581 ttcttgatag taggtttcga tcattgcagt gcggggcgga tcaacgatga ccacctgccg
    11641 ttgatcaaaa gtgttggcgc agtagaggta agagacgtct tctgacgtgc cgagttcgac
    11701 atgcatctga cggccgtccg gttcctgcct tgtcgtttcc ttcgtgaccg tgcgatcttt
    11761 gagtcgttcc cacacgcact gtgctgagtg gccgtggttt tcatagagcg gcgcctcgcg
    11821 atcgcttgag ttcctctgcc ccgcagaccg atcgaacagg acctccctgt ccggctcagg
    11881 cgattcatcc cagtcgaccg acaggtcagc ccccttctgc atcacataca acggtccgtc
    11941 ctccgcaacc tcttcgacca ctcgatagcg aatcggctca ggttgttcga tgagctgcag
    12001 ccccctcgca gccaacgcgc gttgaaacgg aatccgccgc gccagttgtc gcgtcttctc
    12061 gaacacgatg acgcggccat gattcatcaa gacggccgcg agccgatcca gtcgtagccc
    12121 gataccggtt cgctgttcga acgcggcttg ctggtccctg tcgtgcgcac gctcgaacgt
    12181 ccgccaactc cggctcggaa ttcccggatc ttgttcggtc tgcaccagcg cgtgggtggc
    12241 aatgacgaga tcgtaggagc cggccagctg gtccgtctcc acatcgagac actcaaagcg
    12301 aacattcgcc agcccgagcg catccgcctt ttggcgagcc accgcgattg aagccgatga
    12361 acgatcgatc ccgacaacct gtttgtccgg atattgccgc gcgtaaaagg tcgtcagaat
    12421 gcccacgcca cacccgaaat cgaggatggc gtgtgcatcg ccgagacgcg ccgctacacg
    12481 tgagccgatt gctccgtaat actcataccg ctggctgtag aggactggaa gtatctgtgg
    12541 ttgggcggtc agatcataga aggcgatctc atcgcgacga tctccttccc gctttcgctc
    12601 gacctgcgcg ttgagttgat ttagcgcggc tgaggagagt tgctgtcgtt gccaggcaaa
    12661 gtagtccgca tcggacgtga atcgcttaag gccccaccag gcgagatgag acttgagggc
    12721 ttcatcgatg gatggctgtt ccacaccggc ttacggtctc ttcaacataa tggcatcgaa
    12781 ataggcttcc ggcaccggat gcgggagccg caactgtccc gatccgaacg ccacgtactt
    12841 ctcacaggtc agttgctcga gcccgatcgg tcccttcgca tggagacgcg cgttgctgag
    12901 cccgatatcg gcgccggatc cgtagctgtc tcccgcattg agccgcgacg acgcattgac
    12961 gaagacggcg ctggcatcga cctcacgggt gaagcgcatg gccgaattgt aattcgtcgt
    13021 ggcaatcacg gcggtgaggc aggggccatg ctccgcgatg tgcgcgagag cttcgtccat
    13081 gctcgcaacc attttgactg ccagcaccgg tgcctggaac tgggtccgcc agtcttcctc
    13141 ggttgcgggc ttgatcgagg tatgcccggt catggccatc tgccccatga gggctaccgt
    13201 tttggggcag ccgcgcactt caatcttaaa ttcgtcgagg agtcgattga ttaagggagg
    13261 cagaaattgt cgcccgatca cctgctgcac cagtagcgtg tccaatgcgt tggccgcccc
    13321 ggccaactgg gccttcgaat tgatcgcgat attttgtgcc ataggcatgt cggtgtcggc
    13381 atcgacgtag agatgcgtga gccctccgtc gtgacagagc accggcatcc tagctccctc
    13441 cattacggtc ttccggagtc ccgctccacc gcgagggatg atcgcatcga gcacctttcc
    13501 cgatcgaatc aactcgatcg ccgcctcttt ttccgatcgt tccacgatcg tccaagcgcc
    13561 cgcagggacg tcatgctgtt gcgccgcttc ggacaatccc ttgccgatcg cctgatgggt
    13621 gaggctccat tccggcgccc ctcgaaacac cgccacgttc ccggatttga gacagaggcc
    13681 gatcgactcc accgtgatca gcggtttcat ttctgaaatg atgccaatca ccccgatcgg
    13741 cacgcggaca cgggatactt gaagtccatc cggccgctcc cagcgtgacg tgaccgcacc
    13801 gacgggatcc ggctggtccg cgatcacacg aaggtggtcg accatttcct tgatgtcgtc
    13861 ggccgtcatc cgcagccgcg cgacagccgc cttcatgcga tctttgttca catcgctgct
    13921 atagaacttc cccacggcat cgacatcttt ttcattctcc gctagaatgg cctcttcatc
    13981 tgcctccagt cgatccgcca tggcgcgcaa tgccttgtct ttgacaggtc ccgacaagag
    14041 cgccattggc gccacattct ttctgcattc tttcaacaac ttatcaagat atagcttcac
    14101 tggaacttca ggcattgcaa cccccgtcat acatggcgtg aactattgaa caccatacta
    14161 aaacgcgccg gactatagca tgcgattttt ccaacagtaa acaggccgag cgatggagag
    14221 ccgatcactt ctggattcgt cgctcctctt cttctcgagc caggcgccag ccgacccatc
    14281 cccagaagag cgccaaggga accaacagcc aggctatctg gctcgccgtg ataccaaaag
    14341 ccttgacccc ctgcacgacc caccctgttg cggcatcgcc cccgcgcgat accgccgtat
    14401 cgataaagtt cttggctttg tatttttctt cgcggctgac aacagtgaac aaaatctcgc
    14461 gcgccggctt ggcaatggca tactcgcaga tacgcctcac gatggtaaag aggacgaaca
    14521 tgacgagtac cggaatgaac ccgatcatgc cgaacccggc catgctggca agtggcagga
    14581 tgatcaacgc cgacgccaca ccgaaccggc tgatcatctg gctggtgata aagacctggg
    14641 tcagaaaggt cacgacgtta acgaccagat cgatcagcgc aaagagccgc gtccgtcctt
    14701 ccggtgactg gatctgctcg ccgacaaggc gggtttgctc caagtacagg aaggtagcgg
    14761 tggtggtcag cagaaacagg tagaggcaga tgcccaacag atacggcgat cgcaaggcca
    14821 ggcgaatccc cgcccacatg ctcccgccaa tcggctctcc ctgcaggccg gcctgtggcg
    14881 ttgcccgata cctcgaccag cgatccagcc gggcaatgca gaggccacag acagcaagaa
    14941 atccggcgga tatcagcatg agcagcggga cggggaccac ataagtgagc gccgtcgtga
    15001 gcaatggacc gaccattgcg ccggtacttc cgccggccgc gatgactgcg aagagccggg
    15061 cgccctgggt cggcgtaaac agatccgcca tgaagctcca aaagacggag accacgaaca
    15121 gattaaacac cgataaccac acgaagaaga cgcgtgcaac ccaatgcgga gccacatggt
    15181 tgatcaagag cagaaaaaat ccgagcaggt tgatgccgaa gaacgcatag accgtcacca
    15241 acaaccgata gcgcggaacc cgagcggaca gatagccaaa cagcggtgtg accaaaatca
    15301 aactcaggaa ggttgccgtc atcatccacg gaagattctg cacacctcct tcgatcgcca
    15361 tttcatcgcg gacaggacgc aggatgtagt agccgcagag caggcagaag aaatacagaa
    15421 acgaccaaca gaccggcaac agctcgtccg gctccgcgcc ggtaatcgat tggatgctgc
    15481 cgcggacgct cgacataggc cggcatcctg acgaggagcc ggttcccgcg caagcgaaaa
    15541 gtttgctaga atgcccgcga cgaaacgccg ctggaggatc tcgtgcggat cattgacttg
    15601 cgcagcgata ccgtcaccaa gccgacagag gccatgcgcg acgccatggc gcgggccgaa
    15661 gtcggcgacg atgtctacgg agaagatccg accgtcaatc ggctccagga gatggcggca
    15721 gggttgcttg gaaagaaagc cgccctcttc gtcccctccg ggacgatggc caaccaactc
    15781 gctatccgtg tccacacgca gccgggccag gaagtcatcg ccgagagcaa agcccacatc
    15841 gtgcgctatg agcaaggtgc tgcaggtgcg ctcgccggtg tccaattaca ctgggtggtg
    15901 ggagaccgcg gcattatcgg accggagcaa gtcgcagcag caatccggcc caaagatcct
    15961 tacagtattc aaaccgccct tatttgcctg gagaacaccc ataatagtgg tggcggcacg
    16021 atttatccct tggcgacgat cgaacgaatt cgtgccgtgg cttcggcgca tgggatcccc
    16081 atgcacctgg atggagccag gctattcaac gctgtggctg ctaccacact gccaccggcg
    16141 gcctatgcca cccattttga aaccgtgtca ctctgcctct cgaaaggctt gggggcgccg
    16201 gccggctccc tcctgttgac caacgatccg actttgctgg agaaagcaag gcgctttcgc
    16261 cgcatgtacg gcggtgcgat gcggcaggcg ggcatcctcg ctgccgcggg catctacgcc
    16321 ttggaacacc atgtgggacg gctgaaagag gatcacgaca atgccaagcg gcttgcgaga
    16381 aagctgcaac agattccggc gattacgatc aatcctcagc atgtcgacac gaatatcgtg
    16441 atcttcgacg tgatcggcca ccggctggct cccgccgcca ttgtcgccgc attgaagcag
    16501 gagggcctct tgatcaacgc cgtcggcggg acgagtttcc gcgctgtcac ccacctggat
    16561 gtctccacta cggccatcga tcgcgcttgt gacatctttg cccgcgtact gggctagcgc
    16621 cttcgtacat tgacacctcc cccacccaaa tctagaatgc acggcattgg agcaggctat
    16681 gcccttacag gttgtctcgt tcgcgcacat cagtcgtatc ctcgagttga ccgactcgct
    16741 cggcctgaat cgggagtggg tggagattcc cctctctcct gaacaaccgg gctttgtccg
    16801 gcgactgggt aacgggaaat tggaaatcgt ggttgacgca gatcaaccgt tcgaggagtg
    16861 gctgcattcc ctcccacgac agattcaaca agcacaagga actacatcat aatggatacc
    16921 gagcgcgctc ccaccctccc gagccgtgaa gaattgcagg ccgagctcgt tccccttccc
    16981 gaacctcccg agccgccgga gcccctcaat gaaccgggtt ttgaagacac cgttgccaag
    17041 gcgctgcgcc acgtgcgcgg ccgccgcggc ggcgatctgg tcagcgtgct gctggtcggc
    17101 tcaggagtgc gtcgcgccgt gactgctcat agcgatgtcg atctcatcgt gctcgtcaag
    17161 ggacatgccg acggacacga gatcgtccgg atcgcagacc gcatggccga cattcgctac
    17221 tggggccaca aagaagtaga ggaagacctg accttttcgc cgcgccttcc atcgctgctc
    17281 cggaaggccc gcattctgca cgatcatgac ggcatcggcg ccaagctcat cgagcgcgcg
    17341 aaccaacggt tcagacaagg tcctcctccc gccggcataa acgaacaaat ccggttgaag
    17401 gcggagtgct tccattggct cggcaaggct caagacctcg cagataaacc ggcgaccgcc
    17461 cagtatctct tgacgatctt cttcgacgat tttgtgaatg ctttcttccg cctgagaggc
    17521 ctctgggtga cggcaccggt cgatgttccc cgctttatcg cttcacgcga tgtcgacctt
    17581 ggcgacctcg ccggacgttt tctgtcggcc gcgacgctgc ctgaacggct gaattgcgga
    17641 cgggatttgg ccaatctatt gttcaaggac gttccgaatc cagcgcgcat tgattagtcg
    17701 gcatttgaaa aatccgccgg cgcactggag gacgatcctc ggttggggca attgtcgggc
    17761 tgcgtccgga actccgatca gcgttgctga atgatcgccg atgataatga gaccaacgtg
    17821 ccgttcccgc cctcacgatt gcccctgttc atgcgttttc cttctcgccg gagcgaacgt
    17881 tcgccaaaaa gatgccggcg gcgtgatgca atacgtttgt ctgacatgac ataacgcggt
    17941 aggatacagc catgcctcct atttcgcgca tcgacatcaa accgacgcag gaagccctcg
    18001 ctccggtgga gccctgcaca ctcgtcatct tcggcggatc aggcgacttg gcgcgccgcc
    18061 ggttgatccc cgcgctttac aacctcctgc tcgacggcct gctaccagac aattttgccg
    18121 tgctcggcct cggacgcaaa acgatgaccg acgaggaatt tcgagccacc gttcgggaag
    18181 gtgtccttgc ccattctcga caatcgctgg tggaggaccg gtggaagacc ttcgccgatc
    18241 acttgttcta tctggccgga gaaaatgaca atccgaagac ttacgaagca ctcaaagccc
    18301 gcgctgatgc catcgaaggg atgttgggac tccccggcaa ccgcatcttc tatttgagca
    18361 tccccccgag ttcgttcacc gccgtctgcg aagggctgga acagtcgggc ttggcggtac
    18421 gaacgacgag gccctatgct cgagtgatcg tcgaaaaacc ggtcggccgg gatcttgtgt
    18481 cggcccaaca catcaatgcc gtgacgggcc gcgtgttcga tgaatcacag attttccgca
    18541 tcgaccatta cctcggcaaa gagaccgttc agaatctcat ggtcgtccgg ttcgccaaca
    18601 gcattttcga accgatctgg aatcacaaat acatcgatca tgtgcagatt acggtcagcg
    18661 aggccgaagg cgtcggaagt agagccgcat actatgaaga agccggcgcg ctgcgcgaca
    18721 tggttcaaaa ccatctgctt cagcttctct gcctggtcgc gatggaaccg ccctactccc
    18781 tcgatcccga cgtcgtgcga aacgccaaga tggaagttct gcgctgcctg cggccgatta
    18841 gtggcaagga tgtcgacacg tatacggtgc gagcgcaata cgccgcaggc tcggtgcacg
    18901 atcagccggt cccaggctat cggcgcgaga acggggtaaa gcctgattcg acgacggaaa
    18961 cctacgtggc cctgaagtgt tttgttgaga actggcgctg gtcgggtgta ccgttctatc
    19021 tgcggaccgg caaagctatg ccgcatcgtg cgagcgaggt cgccgttcag ttcaaggaca
    19081 ttccccaaat tctcttcaat gccaacgcgc agcagccgca gccgccgaac gtgctggcgc
    19141 tccgcatcca gccggaagag ggattgtctc tccgcatcgt ctcacgggtg ccggggaccc
    19201 gcgcccagac gcatccggtg gaaatggatt ttcagtacag cgacgtgttc ggacggccct
    19261 cgccggaagc ctatgaacga ctcttgctcg acgtcatgac cggcgatgcc tcacggttta
    19321 tgcggcgcga tgccgtcgaa gcctcctggg cttggatcac caaaattctg gaagggtggc
    19381 agcaacaggg ggcgcgatgg ttaccggaat attcggcggg aagctgggga ccggtcgagg
    19441 cagaccggct cattcagcag gacggccgga tctggcgaac tctgtagggg cgatcgtcac
    19501 gacaaggcca gttgcttgag aatttcatcc ttcgtgccga taccgccgat aaatctttcg
    19561 aactgtcccc gccgataaag ggccagcacc ggcaacgagc ggatttcaag ctccgcggga
    19621 atttggaaat tctcgtccac gttcattgtc agaatcatca cccgtcccgc cacccgggct
    19681 tcgagctgct cgagttggcg catcagcgcc cgacaagttc cacagcccgg tttccagaac
    19741 tctatgagga ccggggtcgt tgcgtcttcc accagacgat caaacgtccg gtccgtgacc
    19801 tcagagatca tggccgtatt ccccagcgct tgaaacggac ctggcaaaca ctccatcgta
    19861 gagcaaccgg gccaacagcc gatagcccga cgtcgtcaaa tgcaacccgt cgttggaata
    19921 ctgcgccgca agccgtcgcg tggatggttc cgccgtggcc gcgaacaaat cgatccacgg
    19981 gacgttgtgt gaaacggcgt attcccggat aaggccgttc aactcaaccc tgcggaccaa
    20041 atgctgctcg atccaagcct ccgcatctgg tcctcctccg gtcgcctcca cgcgaattga
    20101 agggaccgtc acaggaatcg ggataatctt tgaagctcgg gccagctcat acattttcac
    20161 gagattcctc atgatatcgg ccggctgtgc gttccacccc aaatcgttgg ttccccccag
    20221 aagaaccacg tgactcggct gctgctgcag gacgtcacgc cgaaaccgca tcgccatctc
    20281 gcccgtgagt tccccgcaaa tcccgctcac ccgaatctcc actgatgctc ccagccgttc
    20341 ctggaggaac tgcccgtacg gtgtctcctg tcccgcagga ttctccggag ttggggattg
    20401 aaatcccgct gtgaggctat cgccaaagca gatgatcatg acagctgagt ttcctgaacg
    20461 atatacccgt cataggctca tggcccatcg tttgaatgta cactggctga attctactga
    20521 gttgtagcct tagaggacaa ccggacgtgc acttctgatc cctgatattg gctctggcct
    20581 tcagttttca ggccatgcgg gagattccaa aacttgtgat ttccttgacc aaattcgagg
    20641 ttaccggtac cactgaacat aatgtctgat ctcaaaaacg cccaagaaat gatggcggct
    20701 gccatggccg ttaagcaaaa tgctgatccc gagctgacgt cggtaaccca gctcctcaaa
    20761 tcactcgacc gggcgtcgaa gaacgtcaga accttcggtc agcgcaaccc cgttgcccag
    20821 aaattttttg cgcagtatta tgcggaactc accgcgcatt tgacccaatt caactccctc
    20881 accttcgtgg tccaacgtga cggcctgttc ctcaaagagg aactcgtcta tggctcgcag
    20941 accggcgatg caaacgaaaa tttcgccttc aagctctaca gcgacgggat ccgggaaatt
    21001 acctttcacc aggacattac cgatgaagac gtgctcttct ttttcgacgc cctctgggac
    21061 accgtcggag acgccggcag tgaagacgac gatattgtga cccgcctgtg ggccaagaat
    21121 cttcccacgc tgacgatcgt gactgccgac gaggtcatga aactttccga acttgacgat
    21181 gtactggccc cacagggaaa agccccgatg gagggctcgt tacgagaaat cattgccgag
    21241 agcaaagcca gggaagtcaa ggacgccagc gggaccaaac agcagaaacg acgtttctca
    21301 tcaggcgtga ccgggtacga ggtttcggaa gaagagttag cggcattggc cagtgagatc
    21361 acggtggaat ccagccgtga caacgtgctg cacatcctcg acatgttgac ggcaatcctg
    21421 tcttctgaga aatctcccga tcttctggac aaactgttcg acatttacgg ggccatcctc
    21481 caatcgctga tccagagagg ccattggtcg gcaatggagc acgtcctggg cctgctatcc
    21541 gaagccgaag cggttcgccc cgacctcact gaagagcaca agaaacacat tcaacaactc
    21601 ttcgaacacc ttgggagtcc tgaaactgtc gatctgatcg gcacatactt aaacaccact
    21661 gacatacctc gcattgatgg gctttcaacc attttcctga tgatgaaacc caccgccgtg
    21721 cccgccctct gcacgctgct cggcaatctc gaacagcagg cccaccaggc gttagtcatc
    21781 aatgccttgg tggagcaggc aaaagatact cctgaggtcg tcgccaaaca cttgacggat
    21841 cgtcgcccca cattcgtccg gaacctcttg gggatcatca cacgatggaa caatccgcgg
    21901 ctcgctgata acgtggaaaa aatcctgcgc tatcccgacc cgctgatccg gcgtgaagcc
    21961 atcaggactc ttgccacctt acgcccgagc ggcacggcga caaaactgat tccgttgatg
    22021 aacgactccg acgaaggggt acgtctcgcg acactgaaat cgctgctcac gggcaactac
    22081 tccgctccct tttcaagttg ggagtccatc gtcaatgccg aagcattcgg cgatcgcccg
    22141 ccggcagaac gacggaacat cttccatgcc atgcgcatga cgtgcggaga tgaggcagtt
    22201 ccttactgga caaccttgat gagtgaatgg ggatggacga acaaaaagaa acgcgaagat
    22261 ctcgccctga tggccgtcga tgccctgggc aaacttggca cgcccgcggc ccagaccgct
    22321 ctcgataccg ggacgaaaaa gggcacatcg gccgtcaaac aggcctgcgc caccgcgttg
    22381 gccggcgtca gcaagcatcc aaggacggcc taacgcctat acgagtaagg aatactgttc
    22441 atggctgaac cattacggac atcagatacg gcttcctctc cggtctccgc gggtcaaccg
    22501 atcctgaccc acgaaaagtc gctgacgaat aaggtcgcga aggggtcaga agccggcgac
    22561 atctacgatc aacaactctt gatgctggga gcccagctgg tcacacagat cagcattctc
    22621 atcaagatgt cacggcttca tggacgcacc aacgcggcgc tcgacaatcc tgtcgaagcg
    22681 gcactgaccg tgattaagac cctggcacac gatgaaccgg cctccatccg cctgcagaac
    22741 gattttcttt ttctcggcca acgccacctg aaagtcaccg catcccagat gcccgtgttt
    22801 tcgatcgtca tcgacgcgtt gaataattgg aaaatcggag gcatttcgtt ctcgaaagaa
    22861 acagaatcgc gcgactttcg agaatttgtc ttcctgcttg cgagcttcga ccccacgacc
    22921 agctccatca aaacattgag aaccgcgctc gaacagaatt ccgtgaaggg cattcaactc
    22981 gaagagcgcc gtgtcctgcg aggcaaagcc acggcgaaga cccgctacgc aaacgccgcc
    23041 gcggcggtcg gcaagctcgt tcaggattcc cgcgagaaag ggacgatcgg attcaaacag
    23101 gccaagcggg cgatccaaag cattgtcgat ctcatgatga acgacgaggc ttcgttgctg
    23161 ggtctgacca cgttgcgatg ccacgaccaa tatacccata accattctgt caacgtctcc
    23221 ttgctctcga tggccttggg taaccgtgcg ggctacccga aggtcgagct ggctgacctt
    23281 ggattggcgg cgttgtttca cgatttaggc aaggccaata tcccgatgga agtcctgaat
    23341 aagcccggtg aatttacgga cgaagagtgg cagatgatgc ggaaccaccc gactgaaggc
    23401 gcgctcagcc tgactaagat gcgaggcatc acgaacttgc ctggacgcat ggtctcggcc
    23461 tccttcgaac accatatgaa tctggacttc tccggctatc ccaagttgaa tgtatcctgg
    23521 aagttgtcga tgaccggacg catcctgatg atcgccgatt gttacgatgc catgacctcc
    23581 tcgcgcgtgt accggcgcga gccgatgtct cccgcaaaag tcctgggatt catgttcgga
    23641 aaggccggca aagcatttga tgccaccttg atgaagctct tcatgacctg cgtgggcatt
    23701 atcccgatcg gcagcttggt catgctggat accaacgaac tcgccgtcgt gttaaaaccc
    23761 gcagccgatc cgaccgacgc cgagcatccg tatgtcaagg tcatcacgga tgcgaatggc
    23821 acggccatcg agaacgggcc ggaactcgac ctccacgaga aagatgaaac cggcgagtat
    23881 cgcttcaaca tcatccggct catcgacaac accgagcaca agttcgatac cagtcggtat
    23941 ttcgtctaga cccctcccgg tcccttgaca ggattccact cgaccgctat tctgaccaac
    24001 ttgtaaccgt aaaagctgtt cactaatctt ctaaggagcg gacactatgt ttaccgtgaa
    24061 gaccctctgg ggtcgcactc ttttcgggag cgtggcaacc ggtgccctgc tcgtcggact
    24121 cgcttcggga gccacggcgg cggacacgtt caaaatgggt gtcatcgatc ctcaggccgt
    24181 gttggaaaaa tcgaaagcgg gcagaaaggc tctggatggg ctcaaagaat atgtctcgac
    24241 cagacagaaa ctgctctcgc gagacgaaga ggaattgcgc aacacggaaa agacgctgaa
    24301 agaatcaggg tccaagctca gcgacgccga gaaaaaggag aaggaaaccc agttccgcgc
    24361 caagattcaa gactaccaaa agcgggcgca ggaatttaac caagaacttc aaggcaaaca
    24421 aaaagagctg gtcgacgact acatgaaacg tatcgcgtct gccacgcaga ccgttgcgga
    24481 aaaaggcggc ttctcgatcg tcgtcgataa gggcagcgag cagaccgtca agatcgtcat
    24541 ctacaacaaa gacaccatcg atctcaccga tcaagtgatc aaggaattcg atcgggtcaa
    24601 cagcaagtaa ccacgctgag cagtgacggg acctccgtca ggtgaagccc tgtcactgct
    24661 ccatcggata gcctgcatcc ctccacgcct taatgcctcc atccatcgag cacacgttcg
    24721 tatagcccat ctgttgaagt gcgtctgcag caagagccga ccgatagccg ccgccgcaat
    24781 ataaaataat cggcgcctgc ttgtcgggaa tcacggtctc gatgtcccgt tcgatgatcc
    24841 ccttgccgat atggcgtgct cctcttgcat gctccttggc atattcatga tcctcccgca
    24901 catccacaaa gtgcacgccc ttctcccgcg caagacgcgc cttcgcctcc ctcacggtgc
    24961 actccttcac tcgcgcgcgg gcggcatcca ccaacttcag aaatcctgca ttgtgtttca
    25021 tcccactgcc gctctcccct ttcagaatag cgtggcctgc tccgcgtgag gttgccgaaa
    25081 cgtgcaaggc accgcctctt cctcgagaat cctgaatccg gctttttttc ttgcgacatc
    25141 atacaactgc tcgatcatag tccagtaggg tccactcccc tgatgacggg agaaaaaggc
    25201 gctgtcggtc agagcccctc gacgaacctc gcggattcga tgagtgatct ttgcaagccg
    25261 gtcaggaaaa gcctgagcca ttcgctccag gaacacgtcc tctaccggcc cggaaagccg
    25321 caacagcgtc gccatcgatt cgccggcgcc ggcctgtgcc gcccgctcca gcagttctgg
    25381 aatatcttcg tcgttgagtc ccgggatcag gggggaaatt gaaatccccg ttggaatacc
    25441 ggcctgtgat aaccgcgcca tcgcatcgaa tcgcctgccg ctcgacggtg cgtgaggttc
    25501 gacgagacgg gccacctcat cgtcggcgaa gggaatgctg aagtagacac gaacccacac
    25561 ctcttgttgc aaccgtttca gcagttcgag atcacgcagc actaagatcc ccttcgtgat
    25621 aatccccacc ggattgcggt actcggcgca cacctccaga caagcccgtg tcagttccag
    25681 agaggcctcc acgggctgat agcagtccgt attgccggaa aacacaataa gttcacctct
    25741 ccaagaaggt ttctcgaatg ccttccgcaa caacgccggt gcgtcgcgct tcatgatgat
    25801 cttggactcg aaatccgttc cggacccgaa tccccaatac tcgtgagtgg gtcgggcgta
    25861 gcagtaggcg cagccgtgaa aacatcctcg gtagggattg aggctccatc gaaacggcaa
    25921 atcggggctc tcgttgcgac tcaagatctc cttggccgca tcttcgtaga gctcgaggcg
    25981 ggccggagat gccggttcga gcagttcccg atattgcaac tcgaatgggt tgggcgggtt
    26041 ggatactgca cgcatgaccg catggtccct ccttttatcg acagtgtcaa ccgctcagaa
    26101 tgcgcccatc gattgactac actacacctc gatgttagat tccagccgct catagagggt
    26161 tctgttccat gtatgatttg cgcaatttgc gggacaactt agacgcgatc cgaaaccaac
    26221 tcgggccacg cggcgccgat gttcagtggg aagatctcag aaaactcatc gaagagcgcc
    26281 ggggacttac cagtcaagtc gagacgctcc gtcacgatct caagaagggg tcggacgagg
    26341 tcgcccgact gaagcgggag aaacagcctg ccgaggaggc tgtggccgct atgaaagcgg
    26401 tcggcgatcg aatagcacag tgcgaaaccg gacttcgggg cgttgaagag aaacttcaag
    26461 atctggcgct gcgcattccc aatctccccc atcccacagt cccggttgga cggagcaccg
    26521 aacagaatgt cgaagtgcgc aggtggggaa caccgccgac gctgggccat ccggccaaga
    26581 cccattggga tatcggagag aacctcggca ttctggattt tgaccgcgcc gcaaagatcg
    26641 ccggcgctcg ttttgccgtt ttgaccggtg ccggcgcaag actcgaacgg gcgttgatca
    26701 atttcatgct cgaccttcac actacggagc acggctatcg agaagttctt ccgcccttgt
    26761 tggtgaaccg atcggccatg acaggaaccg gccagctgcc aaaatttgag gaggacttgt
    26821 tccgtctccg cgatgaagac tatttcctga tccccactgc ggaagtaccg gtgacgaacc
    26881 tccatcgaga agagatcctc gacgaatcgg tcctgccgat tcgttatacc gctttcactc
    26941 cttgtttccg ccgcgaagcc gggtcctacg gaaaggacac cagaggcttg attcgacttc
    27001 atcagttcaa caaagtcgaa ttggtcgcct tcaccaagcc ggaccagtcg tacggggaac
    27061 tcgagcgact caccgggcac gccgagacca tcctccagcg actgggactt cattatcgcg
    27121 tcatggcgtt atgtaccgga gatctaggat tctctgcggc aaagacgtac gatcttgaag
    27181 tctggctccc ctcccagaac caataccgcg agatttcctc ctgtagcaat ttcgagggtt
    27241 ttcaggccag gagagccggt attcgctata aaggtacagg aggcaagaaa gacggtaaaa
    27301 cagatttcgt ccacaccctc aacggatcag gactcgcggt cgggaggacc ctcgtagctc
    27361 tcctggaaaa ctaccagcaa gcggacgggt ccgtcctggt ccccgaagca ctccacgggt
    27421 acatgggagg gctggagcag atcgagaaag aatagaatac tggatgggta tatccagtcg
    27481 ccaagagcta ttataagggg tccgggtcgg ggcaagagtt gataacaagc tagtatctag
    27541 tattatcctg tcgcgcttaa tgttaagcgc tccaggtcga ataacttcgg tttggagggg
    27601 tgacggagcg gccgaacgtg ccggtcttga aaaccggaga cctcgtaagg ggtccgcgag
    27661 ttcaaatctc gccccctccg ccatattatc ccacgttatt ttaccgttat cagatgactc
    27721 ttcccgatgg caccaatgtc attttcacgc tggtcaaagt gggctggtgc tggtggtatc
    27781 ggaagtatgc gccgggggat accacggtag aaagttatgc gcgtattcca ttctagaagg
    27841 gaagtcaaac cagacgacat gggaggatct caggggttga tcgtcaagct caacaccttg
    27901 aaagccgttt gcttcgtcgg tgtcgacgaa tcctgcacgg taaaggtgag gctataggtt
    27961 cctgccgtcc cggccgatgg cgtgccggta atcttaccgg tcgacgtatt caggctgagc
    28021 ccggccggca gtgcgggtgt cacggaccag aaaaagggtg aaacacctcc cgagcgttgc
    28081 aacgttgtac tgtaggactt accgaccgtg gcatttggta acgaagttgt ggtgatcgtc
    28141 agggcggcgc tgacggcgat cgagagagcc tgcgtgtcag actgagccaa tgc
//
LOCUS       DATIFR010000132         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2005094, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000132 DATIFR010000000
VERSION     DATIFR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005094"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1073..1246)
                     /locus_tag="VLD60_07670"
     CDS             complement(1073..1246)
                     /locus_tag="VLD60_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44872.1"
                     /translation="MKCGRCEGLMLEEEIILSGYEVRRKSVSVSHCLNCGRMEYQAMV
                     DTNALQEEDGAPR"
     gene            1468..1866
                     /locus_tag="VLD60_07675"
     CDS             1468..1866
                     /locus_tag="VLD60_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44873.1"
                     /translation="MISRTDMILAAITAMLAQHRSILDGPVPIRAVKLDIKITEDRRI
                     RTVLVSSEIEATVTNGRASSPMHPTAGSLAHLKTLGYHVRVVEKWNSFAKIRHDLLAV
                     DLLALKAGEPVLVIQATREDLRRRSSSSTP"
     gene            complement(1835..>2158)
                     /locus_tag="VLD60_07680"
     CDS             complement(1835..>2158)
                     /locus_tag="VLD60_07680"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HSB44874.1"
                     /translation="ALAQSDTQALSIAVSAALTITTTSLPNATVGKSYSTTLQRSGGV
                     SPFTWSVTPALPTGLSLNTSTGKITGTPSAGTAGTYSLTFTVQDSSTPTPQTAFKVLS
                     LTIAS"
BASE COUNT          486 a          568 c          655 g          449 t
ORIGIN      
        1 tcttcggcgg acttgcggag ttctttcgca gttgcctcgt attcggcgcg attatccttc
       61 gacgtgtcca cggcaggctt aatgtcggcg caagcggaca tggtgatgca caagatcgag
      121 agggcaataa tgaatcgggg cgatatgggt tgctggctac tcatcgatca atctccgttg
      181 gttcctgcac tagtcgtttc acataatgct tgaaccgagc atgggtatgc ggggcgattg
      241 caagattctc caccgcgtgt gggacagaaa ctccaaaacc ccagggaaac gctctactac
      301 gcctccgcca ttcccacggc ggcatcggat ctgcctggac ctcgaacgcg aagaagtcgg
      361 aatgttagtg gcgccagacg cgataaaacc gatgaccgca ctgcttgcag taccatggaa
      421 agcggcgatt gagtctccac atgacgtcct gccagccatg tcgtttaacg cggaagcaag
      481 tcttcgatga gcaatgggga caagtcgtat catcgctgtg tactgcgagc tgattcgtca
      541 ggaggacctt ggtattagcg tcggtcgatc tgcgcagggt gatcatcatt tatctcctga
      601 agggaccaac ggcgagctat ggtgttggag cggcggagac tgaggaatcg gtcacgctgg
      661 ggagggtcag gaagcgcgag ggatagggca gtcagcggag cttcacacct gacaacatca
      721 ccctaggcgc aatgagtgcg ggagtcaaac agtataatga tggcctggat agatcaataa
      781 gacgggcacg ttgcaaacct gacgaaatga gctggcccaa aaccgaacag taggccagtg
      841 gcagcttgag aaacagaccc gcgttgtagg tcgcagcaca ttgcaatgga aagcgccggc
      901 agggacaaat cgcgaagggg ataggggcct cgcaggatgg ttcattctag gagtgcgcta
      961 acattggcgc aaggccggag cgcacggcgt gcggaaggtc tggacggtgc gatgggcgac
     1021 gctgtgaacg gcgcgcttga accgatgcca tgcggaggct ctaagaatta agttaccgcg
     1081 gtgcgccgtc ctcttcttga agagcatttg tatcgaccat ggcttggtac tccatccgcc
     1141 cgcagttaag gcaatgcgaa acggatacag acttccgcct tacttcatag ccgctcaaga
     1201 taatctcctc ttccagcatg aggccctcac agcgtccgca tttcatgttg acctcctccg
     1261 ggatcaggtc agtaaacgtc tcaacttggc atgattagcc cagctggtga acgactaata
     1321 tgttctgcca accgatgttc tgcgcctgac gcaagggcat caatacctca ttgatggtac
     1381 gatgcactcc gacaggcacg acctctcgcg gcgcgaatct actgcacgta ttacagcctt
     1441 gtcatcatcg agctggcggc ctccgacatg attagcagga ccgacatgat cctcgccgcc
     1501 atcaccgcga tgctcgcgca gcatcggagt atcttagatg gaccggtccc gatccgggcg
     1561 gtcaagctcg acatcaagat cactgaggat cggcggatca ggacggtctt ggtttcgtcg
     1621 gagatcgaag ccactgtaac gaatgggcga gcctcatcac cgatgcaccc gacggcggga
     1681 tcgttggccc acttgaagac gctcggctat cacgtccgcg tggtggagaa atggaactcg
     1741 ttcgcgaaga ttcggcacga cctcttggcc gtggatctgc tggcattgaa ggcgggtgag
     1801 ccggtgctcg tgattcaggc gacaagggag gatctcagga ggcgatcgtc aagctcaaca
     1861 ccttgaaagc cgtttgcggc gtcggtgttg acgaatcctg caccgtaaag gtgaggctat
     1921 aggttcctgc cgtcccggcc gatggcgtgc cggtaatctt accggtcgac gtattcaggc
     1981 tgagtccagt cggcagtgcc ggtgtgactg accaggtaaa gggtgaaaca cctcccgagc
     2041 gttgcaacgt tgtactgtag gacttaccga ccgtggcatt tggtaacgaa gttgtggtga
     2101 tcgtcagggc ggcgctgacg gcgatcgaga gagcctgcgt gtcagactga gccaatgc
//
LOCUS       DATIFR010000133        57668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2005293, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000133 DATIFR010000000
VERSION     DATIFR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 57668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57668
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005293"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..976
                     /locus_tag="VLD60_07685"
     CDS             23..976
                     /locus_tag="VLD60_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44875.1"
                     /translation="MSYRYNTTDIIVGVGMCAIVFGALLFFVAANGTYQVAIPQPGLL
                     EEPAGIDVGMSWLQPALGQAIVDQVIFERRANQVIAQSASEWNRATLAHHEFQSLSGG
                     PFGAIMRQAATVPADHMARVQGVMGRAIVNFTQRGIRSGSLSADQYLSDYNSGMIRAT
                     EARGQRLDDNFASTWQATLGRGIVEAVQSYATKAGAIQERMGTALFHLTQAQRSSEEE
                     RAAQQEQFASLIVADIRTEALADRLTLLAAIQSFPEDTAVAFTEPASWPEIPMGYLMV
                     AGFMLAAVFFGGLSLSARSRETKVVDQMKQTANRWVYRIAA"
     gene            1026..1208
                     /locus_tag="VLD60_07690"
     CDS             1026..1208
                     /locus_tag="VLD60_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44876.1"
                     /translation="MSGVYWGIVGGFLAMVATLFVCFDLLYPKAKGSPKASSGRMDEP
                     GEAGTQALDGHRRAAA"
     gene            1260..1427
                     /locus_tag="VLD60_07695"
     CDS             1260..1427
                     /locus_tag="VLD60_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44877.1"
                     /translation="MVEIIGLVTMGALVWLIAWSMGGESESEKRRMSMPHESGRSTEV
                     GSSGARTRHAA"
     gene            1635..2015
                     /locus_tag="VLD60_07700"
     CDS             1635..2015
                     /locus_tag="VLD60_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44878.1"
                     /translation="MKAMIGAVLVAAVVGCFVALGPAMAETAWIDDLANSVSFYKTNY
                     PASNWEPYSQKLGVVRDAVNRGDQRTVKVEMTRWFKMLRVRDHGISDIAADELFNYSL
                     MVTPIQEYGISVPAPSGGVGELAY"
     gene            2108..2614
                     /locus_tag="VLD60_07705"
     CDS             2108..2614
                     /locus_tag="VLD60_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44879.1"
                     /translation="MMTRWLLSTVAMLVFVVPVGAAEPSKDIVTLAAHYGFSGVITKI
                     ESGMLFIHTDNSLQPRTISPNKADRVGLHGAKVGETVNLLVDSGNVMIDASRTDRFFP
                     DHRFVVGTVRYTDPYWQEIQLSTPEGASTYEVDALAGNKLSGLPDGAPVTLELDADNV
                     MIDIHRDR"
     gene            2771..3556
                     /locus_tag="VLD60_07710"
     CDS             2771..3556
                     /locus_tag="VLD60_07710"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44880.1"
                     /translation="MPSRLRMARTVLFLLAAIVATGERPAWAKEAASNTGVALQTILE
                     VEQTARLLAILLDSGRSVVNDHQALYNEPGQSDKGLTPEVFGRQLTDMFRSRSGIDLE
                     DLNSSRLPAQTKKLMKELVAVSRHVVADAQPEIKRSSGPGGLLPAIYGARVASRFTER
                     TGVRMKQTALLPRNAANTPDAAERFALELFADPNYPREKPISETTAQSGLLRLMFPLY
                     ATRHCLACHGEPKGTLDRTGYPREGWKLGQNAGAISVMIPFTK"
     gene            3553..5151
                     /locus_tag="VLD60_07715"
     CDS             3553..5151
                     /locus_tag="VLD60_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB44881.1"
                     /translation="MKHAALSIGLLVIGLGSAWAAERVGGPTEKPAGSGVIVHSDGYV
                     LTAHHVVANARRIVVVTPGEFRVPGVVVSADPEHDLALVKVETVGLSDAPLGYAGSVQ
                     LDQEVVAVGFPFGLREVSVTRGRIAAVRTKGVQRVFQVDAAVNPGNSGGPVFNARGEV
                     VGILTTKFTHPSGIVPEGMAFVVPISYATPLLANIEAFDFTTIGKGIKGQKGANGKGF
                     AQALVRTTVRIETMRVTEGVPPATSATESGGKSDVAVTSAARGQTGGLREPSSVKADD
                     AGVRQVNEQLRLVQREELQRLAERGIVPPEGMILIPAGEFLMGSDDALPDARPLHRVY
                     VSGFWLDKYEVTNRQYRQCAEGGGCAVPKDRSAYDEATRSEHPVTNVTWNQARGYCRW
                     LGKRLPTEAEWEKAARGTDGRLYPWGNNDDVLRVRVRGLEQKLVKNGTEPVGSIDFAV
                     SPYGVADLVLNVSEWVNDWYAEDFYRSSPARDPQGPVRGSFRVLRGGEVSDRPLELRA
                     SYRGWDDVTYWGPSLGFRCADDLP"
     gene            complement(5264..6541)
                     /gene="clpX"
                     /locus_tag="VLD60_07720"
     CDS             complement(5264..6541)
                     /gene="clpX"
                     /locus_tag="VLD60_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSB44882.1"
                     /translation="MFWETRQPSSPPDPFCSFCGKRQGAVRNLIASPAPYTCNSCRTT
                     SPLLICDECLQQCSASLKKDNGAQGSSTSVPLVVPKPTEIKATLDNYVIGQDRAKKVL
                     SVAVHNHYKRILNRDRLKHVDLQKGNILMIGSTGTGKTLLSQTLARILHVPFTIADAT
                     ALTEAGYVGEDVETVVLKLLQNCDYDVKRAETGIIYIDEIDKISRKSENPSLTRDVSG
                     EGVQQALLKLVEGTVCNVPPKGGRKHPEQDYIRVDTTNILFIAGGAFVGLDQIIGQRT
                     MPKRLGFGASDPTSGIDGHTDLLRHVRSEDLLKFGLIPEFVGRFPVLTTLSDLDVPAL
                     IRVLTEPRNALVKQYQALFEIEGVDLKFTDSAVKAAARRAALMKTGARALRTILEEVM
                     LDLMYEIPAHGGVKSVVITEEVIAGIGQPQILT"
     gene            complement(6686..7708)
                     /locus_tag="VLD60_07725"
     CDS             complement(6686..7708)
                     /locus_tag="VLD60_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSB44883.1"
                     /translation="MKPLRVLVLMHKHLVPPQDTTGIETQKAEWKTEFDVVTTLRKIG
                     HETEPLGVEKDLGIIRNTIDTWKPHIVFNLLEAFHNVSLFEPNVVSYLELLRVPYTGC
                     NPRGMLLAKDKALSKKLMAYHRIPMPEFAVFRRGQKIRVPKRLTFPMIVKSLTEESSL
                     GISQASVVEDEQKLIERVGFIHESVGTDALVEHFIDGRELYVGVMGNQRLRIFPVWEM
                     QFTKMPDDVHRIATERVKWSIKYQEKYGIQTAELKDAPEGASERIQHLCKRVYRALEL
                     SGYARIDLRLDKEGKIYVLEANPNPQIAQDEDFAESAKRAGLPYTKLLQQIITLGLQW
                     RPERNG"
     gene            complement(7705..8778)
                     /locus_tag="VLD60_07730"
     CDS             complement(7705..8778)
                     /locus_tag="VLD60_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HSB44884.1"
                     /translation="MATQDKSEPVEDASSDTIRSDLTDWATWPDEKLLDLRLCDLDLK
                     IAGSDLEDRIAQLYRELDGRGLHFHPHFWLSDEWFCPDDVPGIAIPFYLAHPRLAKLE
                     LTQMLEVEGGDPDWCMRILRHETGHAIENAFRLRRRRHRQQLFGKSSEAYPDDYSPKP
                     YSKSFVLHLDSWYAQSHPDEDFAETFAVWLTPNSQWETRYVGWPALKKLQYVDQLMQT
                     LVGRPPLNVSTEEVDPLRRLRTTLRRHYKKKRRHYGLDHPDFNDRDLRRFFSDAAEHA
                     GNMTAVRFLMKIRKPVRRLVAGWTGVYQYTIDQVFEDLIAHCRPLNLRLAVPEEQAKQ
                     EFTVFLTVQAMNYVNTGGRRVAL"
     gene            9220..9867
                     /locus_tag="VLD60_07735"
     CDS             9220..9867
                     /locus_tag="VLD60_07735"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSB44885.1"
                     /translation="MPLYIAILIGALGVGSLYAEEEIIILGKGKTQALAEWDKPVHFG
                     TGDWKVVDDAQHQAIKLRTESSSFALEKSITVDLKQTPYLEWEWKVTVPPAGGDFSSP
                     DTDDQAAQLLVVFPKTFFERRKVISYIWDPTAPQGTIGTAAGPIYLNVKAIVVESGAG
                     QIGNWVTEKRNVVEDYRALFGEMPERAVAIRMQINSQHTRSVAEVFWRTIRFTSE"
     gene            9919..10164
                     /locus_tag="VLD60_07740"
     CDS             9919..10164
                     /locus_tag="VLD60_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44886.1"
                     /translation="MRFLLASGLLGCLIATAAQAEWYVGGYGGISIPGGLSNVTVSNS
                     TLGGGVTDAKINDLELKSNLLWGAGRAISLRRVLGWD"
     gene            10113..10571
                     /locus_tag="VLD60_07745"
     CDS             10113..10571
                     /locus_tag="VLD60_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HSB44887.1"
                     /translation="MGGRAGYFLETRPWLGLEADLYTQKPDVKQQTIVGGTSSGNVFA
                     TSIQETSLRVTVLAANIVIRSPSMSEVFQPYGGVGYGLFFATGSQNGQSNTQISPGFQ
                     FDAGARYVLTPEWALFGEFKYNHATIRFSGIKGNYDSQNFNVGLMYHFGR"
     gene            complement(11148..12101)
                     /locus_tag="VLD60_07750"
     CDS             complement(11148..12101)
                     /locus_tag="VLD60_07750"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44888.1"
                     /translation="MWLLLPFLLLLDQNPAWSLPHSASVQPVSSNSTLTGDELLRIGE
                     IHDHQLHFRETLTYYELALSTFREKKQTRGIATALVKIARVYERQGKIHDAHTAVQEA
                     VRLLAKSSDRMAYAQSLSVMGRISARLGYQQEAHTSLSQAIALFNRLKDRRGWNEASI
                     QLGLLQVSDDFPEQGLSLLEQARDDARTREDHSQHLAALMALGTAHWLLDHPHDARRF
                     YEAALRLAEAERNMPVEAALQLRLAYFEGEDGQLSEGVEAAKQAVFLSQTLHDAEIEA
                     AATSLLADLYRKMGRMTEAEESEQRALSMYRHRQIHVHALR"
     gene            12417..12611
                     /locus_tag="VLD60_07755"
     CDS             12417..12611
                     /locus_tag="VLD60_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44889.1"
                     /translation="MFSAWDFNKWMISAGIVLLLASLIAFFAAVVTEIIIRIWGEERG
                     VVPPLRMSEAEQKAPSRKAA"
     gene            12797..13219
                     /locus_tag="VLD60_07760"
     CDS             12797..13219
                     /locus_tag="VLD60_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44890.1"
                     /translation="MGKINWEKVFLGGLVAGIIIDVVEGILEGVILGPEWRQAMQALG
                     HPLQETGVNMTLHILLGFAYGLTALWLYAAIRPRFGSGPKTAFYAGVGVWVLGSLLPS
                     VNWGPRGLLPGHLFTIAVIVGLVEIVVATEAGAWLYKE"
     gene            complement(13386..13937)
                     /locus_tag="VLD60_07765"
     CDS             complement(13386..13937)
                     /locus_tag="VLD60_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44891.1"
                     /translation="MRRLITYVSQISGLGGMRGVVVVALGLFMSSSLYAADLTVGENS
                     FGCIRDWTKVRNTYITHADPEKLKEAVRIFKNSVSDKEYPVGTFLQLLPNEAMVKHPR
                     QKFPETNGWEFFDLDLSAQGTKIKTRGEQTVNFQGVKCFSCHQPAIKYDFVCERTHGC
                     APVPLDDQKIAELQAADPRCSKK"
     gene            complement(14159..15397)
                     /locus_tag="VLD60_07770"
     CDS             complement(14159..15397)
                     /locus_tag="VLD60_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44892.1"
                     /translation="MTPEEARAHYNFLLTLCIRKAESFGPMAFTFVKDRNFQTLGLTP
                     EEQFNLLMAVAESFADEPKRFTHKLDCLQKAADILPQTSFFDPGLARDIRQEIQRLTI
                     ELDFYTAAMKPDRAGPGPEFADRQIIIETDMPDYFFSIAQQRASAYYQEKYRLPKEAK
                     IAQHFSSPTRRFEPDTTTVHKEYPGACAPFVSARTGAWHIMLPFDLKISRTPDDPLDA
                     GLRIWYAKMGYSFPLRYELGRLCSYYDDQVLDIDMADPHLLFVSVSPLKEQNLGTVTR
                     AVASDVPFEIGLPRAFLEGTNTLGPYVQIGCNIKVWFDAAVMALLVQGAPDLYEYGLG
                     GAAGLLTRTYASEKTAAYAGAGSRSWQQGLSFNFINMHLQLLPNVTSGVVPANTPIFS
                     VYPTITRGQYQLTDARTLTS"
     gene            complement(15530..15679)
                     /locus_tag="VLD60_07775"
     CDS             complement(15530..15679)
                     /locus_tag="VLD60_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44893.1"
                     /translation="MAIGILAFYHAIGSLSFAFTGQAILPLGPSLLFTDTVAVARPEL
                     RRIPG"
     gene            complement(15788..16294)
                     /locus_tag="VLD60_07780"
     CDS             complement(15788..16294)
                     /locus_tag="VLD60_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44894.1"
                     /translation="MSLCPRAIQLLMFASLWAMTACALGEQSTGPLAEYLQSPQGSAE
                     WKSTEQQLAREAEAAATEYRLIKTLDALRPYETAVRACLDHGFLLYRVLDASSSDFPK
                     GFRSSLEDRASELMDIADEYLKLGASDIIAVGIARDVIHKYNVERMDRAQRRAEGILM
                     QYRYQRNY"
     gene            17108..18715
                     /locus_tag="VLD60_07785"
     CDS             17108..18715
                     /locus_tag="VLD60_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HSB44895.1"
                     /translation="MEGLHHLEIVILLLALVLTLTTVAQKIFIPYPILLVIGGLILSV
                     IPGLPAVTLSPDLVFLVFLPPILWAAAYFTSWREFRGNLRPISLLAVGLVLATTAAVA
                     AVTHAMLPGIGWAEAIALGAIVSPPDAVSATAIGRRLRIPRRIVTILEGESLVNDATA
                     LVLYRAAIGVAVGGSFVLGETLLQFIFAAVVGVALGLAVGMAARWALCATEDSFTQTA
                     ITLLAPYVAWVLGELTQASAVLACVTGGLHVRQHFSAAVAPTTRLQARAVWDLLVFVL
                     NGFIFILIGLQLRVLREAVPSGRFGQLLMAGALVCVTAIVVRLGWVPLAAILPRALSP
                     SLRARDPMPRWSSLFLIGWTGMRGIVTLAAALALPFTTAGGQPFPFRAEIILISFSVI
                     LATLVLQGLSLTPLIRVMKLQEDRGQEQEERQAREHAATVALRRLDELAGEEWLNADY
                     VDRLRVHYGRQLQRFASSGTVDPDCSLEAGDTFRRLRHETLSAERLALIALRNDGTIS
                     DELLHRLEHELDVEALRLGIGELRVSR"
     gene            18866..19228
                     /locus_tag="VLD60_07790"
     CDS             18866..19228
                     /locus_tag="VLD60_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44896.1"
                     /translation="MKKDSWLYGVALVSGVVVWILVSSFSGKREAWDSDLYFTFGIPV
                     LCLVAGLLGYVEPNRPWRWGIDPLFGQGVWMLVSQGFGNLFPLGLLVLGLFAVPSIVT
                     AKVGAAISKRVTRGSNFH"
     gene            19351..19611
                     /locus_tag="VLD60_07795"
     CDS             19351..19611
                     /locus_tag="VLD60_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HSB44897.1"
                     /translation="MQGQSDTLIRDRLARQRTELANERTLLSYIRTALGFFIVGIPAV
                     WWLELPGIQALGVVSLLAGVGFLGVGVWRFYSIKAVIDQQPD"
     gene            19703..20062
                     /locus_tag="VLD60_07800"
     CDS             19703..20062
                     /locus_tag="VLD60_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB44898.1"
                     /translation="MQRNWIVRVVTVGLVSGLLFGTVAQGQAESKAKSGDVARGKTLF
                     VRNCAGCHGSQGKGDGYRILGADPADLTSPSITKKSDADLLKTIHDGKPNMPAWNVHL
                     SEDQSRDVLAYVRTFAK"
     gene            complement(20291..20656)
                     /locus_tag="VLD60_07805"
     CDS             complement(20291..20656)
                     /locus_tag="VLD60_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44899.1"
                     /translation="MAVVLITILMGLTMIAPGSALADDQMGGTNDSEGTGIQVQSWLL
                     TVPYCIGKSAFAISGAVVGGLSYLFSGGNVQTAQSVWTTSINGTYILRPAHLRGEEPI
                     HFLGKAEGDGSEPLRQASK"
     gene            complement(20769..21287)
                     /locus_tag="VLD60_07810"
     CDS             complement(20769..21287)
                     /locus_tag="VLD60_07810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSB44900.1"
                     /translation="MKNTHRNNIAIVALITVLICLGLLSGQSLATEKSAGSPRIIDGS
                     NVTLMFQITIPGEQGFIVRDIGKFVQGQHQLLPALERVVTGMKSGDEKNVELSVEEAF
                     GPYDGRKKKTVPRDELPPQTKEGDVLQDRAGSQATVAQLSDTAAVMDYNHPLAGKPLI
                     VKIRILRVDNPS"
     gene            22761..23249
                     /locus_tag="VLD60_07815"
     CDS             22761..23249
                     /locus_tag="VLD60_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB44901.1"
                     /translation="MTRPVKPVPAGYHTVTPTLTIRGVDKALDFYKLAFGAEERMRFF
                     APDEKTIMHAELMIGNSIIMLNEEYPGMGCRGPQALGGTSVGLYLYVQDADHLFNRAV
                     SAGAKVDMPVTNMFWGDRCGQVTDPFGHKWTLATHKEDLSAQEMRKRAEAFFAQMALE
                     TR"
     gene            23494..23925
                     /locus_tag="VLD60_07820"
     CDS             23494..23925
                     /locus_tag="VLD60_07820"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HSB44902.1"
                     /translation="MTTLTNRFSSLGRVWCSRVPGFVLCGALALSCSGCLSLAVGAAG
                     GAAGAVYVMGKLKDEQSYEMSVVHKATIAALDELQLKALEDKVDKLSAHVESEFADGE
                     HVWIDLESLSDSRTGLTIRVGVTGNEVRARKIYDTIKRHLA"
     gene            24133..24795
                     /locus_tag="VLD60_07825"
     CDS             24133..24795
                     /locus_tag="VLD60_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44903.1"
                     /translation="MIQTESTEIATQTRERTCKRAVGQAITLAMTSIALLFALASCAK
                     FFDMSGERITVPGPEWGVVIGSVLVQPEKIASDATGKPVDVSGSSYEFEIVRIQPGDP
                     DGEGPYVEKYRLDVKAGEERMFISRLRAGQFLIKSFRDEQMTGLGGDLGLVFSSMGGE
                     IRYIGRLLVEIPRQVSKGKRYGFTVENAREPTMAEVSRHHADLTKDVVDKPMQARERP
                     AE"
     gene            24931..25662
                     /locus_tag="VLD60_07830"
     CDS             24931..25662
                     /locus_tag="VLD60_07830"
                     /EC_number="1.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004128 - cytochrome-b5 reductase
                     activity, acting on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-cytochrome b5 reductase"
                     /protein_id="HSB44904.1"
                     /translation="MSGPITRIKTKTPVPYELTAIHHDTHDTKTFCFALPDDATLDML
                     PGDHLYIHATIDGKAVKRPYTPSSTPGITGYFDLTVKRYETGAVSKYLHDREVGDTVL
                     MSGPNPGGHWVDGMAKKAGFVAGGTGITPMISIIRWILARSLPVELFLVFANKAEADI
                     IFREEWDEDEREHASFHCYHVLEKPPAGWSQGTGRITETVLRKHLPSPGPDTVIFLCG
                     PPPMVDVLEATLKTIGYSEQAIILP"
     gene            25815..26345
                     /locus_tag="VLD60_07835"
     CDS             25815..26345
                     /locus_tag="VLD60_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB44905.1"
                     /translation="MPRPVLILPGIGNSGPQHWQTLWEQRDSHFQRVNQRDWLRPVCD
                     EWAGVLDGAVRACLSAPVLVAHSLACLLVAHWASRSKRVIHGALLVAIPDPDAPGFPA
                     DARGFAPVPLCPFPFPSIVVASTDDSFGSLAHAKHCAAAWRSDFVEIGAAGHINADSG
                     IGDWNQGYALLQRLLA"
     gene            26402..27586
                     /locus_tag="VLD60_07840"
     CDS             26402..27586
                     /locus_tag="VLD60_07840"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HSB44906.1"
                     /translation="MADTANETSGYQGPAFFSYGFRPFFLGAALFAGVAIPVWLLMLG
                     GVGGLDSVYPARDWHVHEMVFGFLPAVITGFLLTAIPNWTDRPPIRGRELIGLWILWL
                     AGRLFIAMPWLMPVVAAIVDAAFLVAVATLVWREIAAAKSWFHAPMGALISLYAGANI
                     LFHVLALSGAEIDLPVRMALALVMVLLALIGGRVTPTFTGELMAQQGLSQQPAAFSRF
                     DGLSVVLVAIAALIWTVQPHVLATGWILVAAGLINLGRLSRWHGWVTWREPLVLILQL
                     GYGWLAMALLVLGGSILGFGLETADAVHALTTGAVGVMTLGVMTRASLGHTGRPRHAG
                     PLTVCIYTLVTLGALMRVVGPTTGLPTNTFLGLAALCWSSAYLLFVIVYGPFLLRPSL
                     DE"
     gene            complement(28077..29498)
                     /locus_tag="VLD60_07845"
     CDS             complement(28077..29498)
                     /locus_tag="VLD60_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44907.1"
                     /translation="MTYNHVINGVLVLSLGTIPFAYGLESPSDQERFTPQIAAQKGGI
                     ESGTVADQAATAKQRNPAKDQRADQNAQVTLGGAKAFVSGEIKKIEGDYYFIKDDEQG
                     DEVRLLVTKDANLDCSAATTPTQATSSKSVETDRMSAELQAPDASDRQKEQGQKKDET
                     AMGSGFRIGACSFKPGDRIKAEVDDMGRVTTFRFMPGARSTEPRTARSLGESAGTGEL
                     AIPGKQDKPGQLDMTGPQGYPPKQYAILPVPVGELKSAGRDSLLHSPVKNSDGVLLGS
                     LENLIMDSNTGQIEYAVVLLNDTNTLETVPWAHMKVKRDTDTSELVVDTRHYQVSPDL
                     TNKEMVDRSPELEKMLKDMQSAKAPGDLRDAEGADGKTPAMKSKRSSELCANKDCRVV
                     RGRVSQLEGNSFTIKENSGSGKEVRMTVDQKTQKGQVGIGGRDEEFVVGDTIEAYVTP
                     TGHAETINLLRPDSSGRPEEAGG"
     gene            complement(29495..29824)
                     /locus_tag="VLD60_07850"
     CDS             complement(29495..29824)
                     /locus_tag="VLD60_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSB44908.1"
                     /translation="MTAAIALLGLGVSLPFMGPTEENTRFQSIPISMSQAVEAAEMVI
                     PGQAIDARLEIVEGSPVYAIHILSIDNSLSTVKVNGEDGEVTVVLDSDEQQQVDELIR
                     SEQGGSI"
     gene            complement(30800..32032)
                     /locus_tag="VLD60_07855"
     CDS             complement(30800..32032)
                     /locus_tag="VLD60_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB44909.1"
                     /translation="MKRTLLAGAIGNVLEWYDFALYGYFAPVFAALFFPSDSPSISLI
                     SAFGVFAVGFLARPLGALLFGYWGDTLGRRNALAWSVLFMAIPTCLVGLLPTYATIGV
                     VAPIALTLCRFLQGLSVGGEFTGSATFLVEHAAASQRGYVGSWAGFSAQIGALLGSGV
                     GALTASSLAEEALRQWGWRIPFVMGGFIAVVGWYLRTKVPESPAFEAIRQAGNVTASP
                     VRDVFTMQRRAVAKVIGLVWLHGVAFYLLYVYLTTYLVTVTTTPLATVLVLNTSCMTI
                     LALLIPVMGAWSDRIGQPPLLIAGAAGIALLSYPCFLWLTSQDLPRMITAQIVLTILV
                     ACYMGPFFAAVAELFPTSLRYTGLSIGYNIGAALFGGTAPLIATVLIEWSGNSLAPAF
                     YLSLCATISLAVALTIRK"
     gene            32321..33052
                     /locus_tag="VLD60_07860"
     CDS             32321..33052
                     /locus_tag="VLD60_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB44910.1"
                     /translation="MTTVAEIAPDLYRISTFVPDFNLQFNQFLVRDEEPLLFHTGPRA
                     MFPAIRDAVATVLDPATVKWIGFSHLEADECGSLREWQQVAPKSMALCGLIGKMVSID
                     DCMAARPAVGMKDGEVMSTGKYRFRFLHTPHVPHGWDAGLLFEETQRTLLCSDLFHQA
                     GEVEPWTHSDLLGRCRQVLVEYQQGPLANYVPYSTLTDATLHRLAGLQPKTLAAMHGS
                     AFVGDGARALRDLAVMFKEVLGSST"
     gene            complement(33509..33733)
                     /locus_tag="VLD60_07865"
     CDS             complement(33509..33733)
                     /locus_tag="VLD60_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44911.1"
                     /translation="MRYLLALLVGFTAWWPTFALAEGAGGTYRGIATMYYTLMWVVLC
                     YGLYDTFGKTALYVGAPILAIATYFALPPG"
     gene            34108..34368
                     /locus_tag="VLD60_07870"
     CDS             34108..34368
                     /locus_tag="VLD60_07870"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB44912.1"
                     /translation="MGPMRPKPSKKISKPAADRAKATSQIPLPEELHEQIAKRAYELY
                     ERRICKGALDDWLQAEQEILRETKTRKAHPPHRGGYASEEQE"
     gene            34919..35308
                     /locus_tag="VLD60_07875"
     CDS             34919..35308
                     /locus_tag="VLD60_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB44913.1"
                     /translation="MPTFLRAYAPHTYALMRIVTGFLFLWHGTQKLLSVPVPPPPQVP
                     SFVIAIAGPIELIGGSLVMIGLFAGWAAFLCSGLMAFAYWMAHGTQALLPLQNMGELA
                     ALYSFVFLYISAHGSGIWSVEAARNAS"
     gene            35429..35653
                     /locus_tag="VLD60_07880"
     CDS             35429..35653
                     /locus_tag="VLD60_07880"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSB44914.1"
                     /translation="MRHKRRIHDAIAGLPVTAGVVLGHYVSAHWLILPAVIGVTLIQS
                     GLTGFCPVYFALDRLLPSERAADASSCCAK"
     gene            35798..36277
                     /locus_tag="VLD60_07885"
     CDS             35798..36277
                     /locus_tag="VLD60_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB44915.1"
                     /translation="MAIRITLAQSSDADVIASMVGKLLHEIMAAVKHKAFGFHQGETA
                     TRVRTWIKDGAYTVLLARQERVAEPVGFLALYESYALYAEGKFGTIPEFYVSPAHRSK
                     GVGSALLLEAKRTGETRGWGRLEVTTPPLPEFDRTLAFYQRHGFSVSGGCKLKVALL"
     gene            36286..36690
                     /locus_tag="VLD60_07890"
     CDS             36286..36690
                     /locus_tag="VLD60_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44916.1"
                     /translation="MQLDRTGCGLASVAVVAGVRYRQVQRVANRLGIFAEDERLWSET
                     GYVRRLLRYYGVRAAQTEVRFRSWEMLPDLALMAIKWHRVHGRAFWHWVVFWRGPGGP
                     VVLDPKRGLRTNARTDFGRIRPKWFLAVTTSK"
     gene            36772..37092
                     /locus_tag="VLD60_07895"
     CDS             36772..37092
                     /locus_tag="VLD60_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44917.1"
                     /translation="MPILLALFLVFALVPQAAALEGSQPTASPMIKGDVLYWEGEEVI
                     VKEMSGREVRLRVTPETKLEGVISRLKTGDKITAQVNSDGRALSITLQIPDSGPSPTT
                     PGLR"
     gene            complement(37145..37435)
                     /locus_tag="VLD60_07900"
     CDS             complement(37145..37435)
                     /locus_tag="VLD60_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44918.1"
                     /translation="MKSTLEIFAKDVEAIASEAMEFFGKNHDPAFVAEQFKNDVPYSI
                     EFGKDLERLIILLGLRIGREERTLYSEYDRSASRTRVAWLGQWAIRVGLDDG"
     gene            complement(37490..37636)
                     /locus_tag="VLD60_07905"
     CDS             complement(37490..37636)
                     /locus_tag="VLD60_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44919.1"
                     /translation="MSQLIEQRKSDHATRKQTLKKTVDFTRPATERIAILNDATGALL
                     SHLE"
     gene            complement(38069..38650)
                     /locus_tag="VLD60_07910"
     CDS             complement(38069..38650)
                     /locus_tag="VLD60_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HSB44920.1"
                     /translation="MTRIQLLCLFLLTSSMVACETAKVGYDYDPSANFSDYHTYEWAV
                     PKQEKTGDRRADSPDVDIRIRTAVGAQLHLKGYTKPQNKQPDFYVAYHIGVNSMSLDS
                     STQYFSEGMTGHPFTYSADTRTMGKKQQGPVSDKPLYMTGALLIDVIDASSKKLVWRG
                     TASGEINPGLTSEERDERIRGLVHEMFSHFPPK"
     gene            complement(38828..39367)
                     /locus_tag="VLD60_07915"
     CDS             complement(38828..39367)
                     /locus_tag="VLD60_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HSB44921.1"
                     /translation="MTRLQSLCLFLLAPLVAACASPKVGYDYDSSANFTVYHTYAWVA
                     DKQEVTGDQRLDNSLVDGRIRTAIGTQLRSKGYTMSVNKQPDFYVAYHIKVKDMMKGS
                     STQDYIGDRAHGTYTTISDIQPYKEGTLLIDIVDAASKQLVWRGSALAEVDPAMTPEE
                     RDKRINTVVHAMFAHFPPK"
     gene            39655..41262
                     /locus_tag="VLD60_07920"
     CDS             39655..41262
                     /locus_tag="VLD60_07920"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HSB44922.1"
                     /translation="MMTTDTSLQNELAAATGKRRTFAIISHPDAGKTTLTEKLLLYSG
                     LIRTAGMVRGRKGGKTAASDWMGMEQERGISITASAMQFPYKDAVINLLDTPGHQDFS
                     EDTYRTLTAADSAIMVIDAAKGVEAQTRKLFAVCRMRRIPVLTLINKMDLPGRPPLDL
                     MTEVEQALNIHASAINWPIGAGSDFVGIVTRADSRVQLFSRTAHGGSTKVDVANLLLA
                     DLASSGKVSDEVIAEVRHDLDLLDIAGNPFAREPFLAGEVTPVFFASALTNFGIESFL
                     DAFVELAPSPSARLADRDDGTELTVDPIEMPFSAYVFKLQANMNPKHRDSTAFLRICS
                     GRFERDMMVKHHRLGREIRLSRPHSLVAQERSTVEEAFPGDIIGIINPGLFAIGDTIS
                     VTGGFNFKPLPQFQPEIFARIRPTDVSKRKAFDKGMEQMAQEGTVQIMKSLNDLESLV
                     AAVGRLQFDVLQYRLRDEYRVETVLDALPYTCSAWLEGDPGTFKSPSVSMMVKDQRGR
                     VVVLFGDQLMKTIARDRNLNHVLKDMG"
     gene            41572..42720
                     /locus_tag="VLD60_07925"
     CDS             41572..42720
                     /locus_tag="VLD60_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44923.1"
                     /translation="MMGIRILVADDDPDIVLSVSERLRWMGYEVITASDGCAALSAVE
                     SHSLDLVLIDVSMPRLSGIEVLKWIRPRWPNLPVVILTAYGTIRLAVEAMKEGAVDFI
                     AKPFEHGQIDTVVTTVLGRSEQRVEMTQLMGEITHDVKNLLMPLVTGTDLLAEEIDDL
                     FKNLPETESVQAEKSHHVCEEVIHLLRNTSRRIQDRMKGIADYAAVARASQKFEPCRI
                     AKIAASVMKSLRVLAEQKRITVRFEGLDKLPDIVGDEPRLYSLLYNLVNNAIPEVPPL
                     GSITICGRHRADDDFIELAVQDTGNGMPPEIRDSLFTNRLVSMKAGGTGLGTRIVKDV
                     VDMHGGQIAVHSQEGNGTTFVIRLPIHPLALVQKAAKGLSESQIAHPD"
     gene            complement(42811..43407)
                     /locus_tag="VLD60_07930"
     CDS             complement(42811..43407)
                     /locus_tag="VLD60_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB44924.1"
                     /translation="MDHQRELDEILERMISDYVAHNSAAKILKTMLDDTGVGFVPVVD
                     HVTLRTLDIDRRAEQFVAFGYTYDETLHYDDWYAKVYRKAGYPALFVDQGYPDDRGKT
                     SIIPGWVKKFGDQVFHHVAVRVEDIEKAIERLRKKGVVFAGKIVGPPGGELRQIFSAP
                     EMVDGQPFSVLELAERHRGYQGFLPPQADSLMKSTAPR"
     gene            complement(43440..44969)
                     /locus_tag="VLD60_07935"
     CDS             complement(43440..44969)
                     /locus_tag="VLD60_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB44925.1"
                     /translation="MSTVHAALKDLGIQAVNSGGSTGSGWWSDQTPGSLLQSINPASG
                     EVIAGVHACSANDYDCIAKQSREVFKSWRSVPAPIRGELVRLIGQALREKKDQLGTLV
                     SLEVGKIKAEGDGEVQEMIDMADFAVGQSRMMYGATMQSERPAHRMSEQWHPLGPVGV
                     ITAFNFPVAVWAWNAFLAAIAGDTVIWKPSPKAPLCAIAVQHICNQVMERQGYTGVFS
                     LFITEQAALAEVMVKDVRLPLISFTGSVEVGRRVATAVAQRLGRTLLELSGNNAVIVD
                     ETADLDMAVRAILFGAVGTAGQRCTTTRRLLIHESRADELTDKLVKAYSHIPIGNPLE
                     PNVLLGPLIDQAAVQAYRQAIDEVKKEGGEILYGGRVFNRPGNFVEPTIVRARNHWPI
                     VQRETFAPILYVMAFHTIDEAIRTQNDVPQGLSSAMFTNNLRHGERFLSVAGSDCGIA
                     NLNIGTSGAEIGGAFGGEKETGGGREAGSDSWKAYMRRQTNTVNWGTELPLAQGIQFG
                     S"
     gene            complement(44966..46054)
                     /locus_tag="VLD60_07940"
     CDS             complement(44966..46054)
                     /locus_tag="VLD60_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HSB44926.1"
                     /translation="MHRALVLGAGKIGSLVTCLLAQRGNYEVHVGDVNLDAPKRLVED
                     LGLEHVTPSILDVRHPDAISTYLSAHPVDAIISSLPYYCNPTVAGLALTHRVHYFDLT
                     EDVEVTNQIRILSASATRAFMPQCGLAPGFISIVTNNLMTHFETLDTVKMRVGALPVH
                     PSNALKYSLTWSTDGLINEYGNLCYGIEEGEKVPLQPLEGYETIELDGLLYEAFNTSG
                     GLGTLADSYAGKVQTMNYKTLRYPGHCEKIHLLMKDLKLNEDRETLKRVLEHAIPQTL
                     QDVVLIYASVTGTKDGGFFEENYVKKIYPQCIKGKLWSAIQVTTASSVCCVMDLVLKN
                     PSRYHGFITQESVPLKDFLANDFGACFR"
     gene            complement(46140..46466)
                     /locus_tag="VLD60_07945"
     CDS             complement(46140..46466)
                     /locus_tag="VLD60_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44927.1"
                     /translation="MDPGQDNHVVELRLSYRYVKEHAWVVQAINGFLSAYFMEQPSFR
                     VQRHFDELESGMHVWLCEIPSSMKMKTLLRRLQSDIPPFQCHESPSGSNSRPQFVLDC
                     SSTPAH"
     gene            46576..47415
                     /locus_tag="VLD60_07950"
     CDS             46576..47415
                     /locus_tag="VLD60_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB44928.1"
                     /translation="MTLTAYNQVPVPSFMYGTAWKKEATASLVLQAVEAGFTAIDTAN
                     QLIHYDEALVGEALLQLARQGITRDKLFVQTKFTPVDGQDHRTPYDVKASITTQVQQS
                     FESSLVHLHTDSLDSYVLHGPYSRRGLGAEDWEVWAALEALYETGRTKMIGISNITAE
                     QLSLLCNHAKHKPMVVQNRCYAAFGWDKAVREICRTHGMIYQGFSLLTANREVFTEPD
                     VHAMAARYGAGLAQIVFRFAMQVGMLPLTGTTNQQHMKEDLRSDQFTLTPEEVRRIET
                     IGM"
     gene            47415..47810
                     /locus_tag="VLD60_07955"
     CDS             47415..47810
                     /locus_tag="VLD60_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HSB44929.1"
                     /translation="MAGIKVRVKRIYEPVSKADGFRVLVDRVWPRGISKAGARIDLWL
                     RDIGPSTALRRWFNHDPVRWKQFCTRYRAELRGKPQLLALLKQHARSGAVTLLYSARN
                     EECNQAVALCAMLKQPRTRHVRVRQRRSV"
     gene            47807..48388
                     /locus_tag="VLD60_07960"
     CDS             47807..48388
                     /locus_tag="VLD60_07960"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSB44930.1"
                     /translation="MNPWPKIRCGWAGSKPHLILYHDREWGMPVHDDQRLFEMLLLEG
                     AQAGLSWETILRRREGYRQAFAGFDPVKVARFDRQKKGALLKNGGIIRNRLKIEAAVT
                     NAQAFLAVQQEFGTFDRYLWQFVGGSPRINRWRKMSDLPATSPESDALSKDLKKRGFR
                     FVGSTIVYAFMQAVGMVNDHTVDCFLSKKHSRT"
     gene            complement(48418..48825)
                     /locus_tag="VLD60_07965"
     CDS             complement(48418..48825)
                     /locus_tag="VLD60_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB44931.1"
                     /translation="MLSSLYGSATDFKRRAWPAQTEHFLRPMEQRKYQRFTDSSLVTF
                     SSETINGEGRLDNLSLGGAAILSDTAIARGEYLTVTITFPAQAGAIEIELAPVRWVKT
                     GSFGVEFIRIAPDSQQRLKQYLNTLENTSTDAA"
     gene            48856..50124
                     /locus_tag="VLD60_07970"
     CDS             48856..50124
                     /locus_tag="VLD60_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HSB44932.1"
                     /translation="MWTGKVVERRPAMKVAVIGGGPAGLMAAEAARAAGAMVEVYDAM
                     PSIGRKFLLAGKGGLNLTHSEPLEPFLARYGPRRGQIEPLLRSFGQEALRAWVHGLGI
                     PTFIGTSGRVFPKDLKAAPLLRAWLRRLRASGVQFHVRHRWRAWDDRGALCFTTREGI
                     KRVEADATILALGGGSWPKLGSDAAWVPFLKQRRVDIAPLRPANCGFDIKWSDHLRTK
                     FAGYPVKSVAVVVKNEQGGESWHPGEFVITETGVEGGVIYLASAILRDEIERNGTATL
                     WLDLAPDRDLPRLTRDLSKPRGKRTVASHLRRYARIEGVKAALLREVIPKQDFTDPAR
                     LAAAIKRLPLTLVATRPLDEAISTAGGVMFESLDERLMIRHLPGVFCAGEMLDWEAPT
                     GGYLLTACFASGRVAGTGAVDWLKAARHRR"
     gene            50325..53171
                     /locus_tag="VLD60_07975"
     CDS             50325..53171
                     /locus_tag="VLD60_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HSB44933.1"
                     /translation="MMRAIQLGTVVSIVGLLLAACSLDVFTSEVACVQTFISGDIQHV
                     GQLRTERFLGKVSETRARCLGGQYAVDYRSGPWLDWPNYWATRDASSRVPLSLFSDAK
                     LIGPNAHGINAALYELELQRIELIKFNLFDNNKTYEAYVKGRGDDPGPILKTWPEMRL
                     AEAHHNYKDVGGAAPQQICTGDTIRFRSVTGICNDIYNPLMGSTHQLFARNAEFETTF
                     PDLGLEEITRNRHGDRLGLLKPDPQVISRKLFTRNESHPESCHNGYGDGTAQTDCEYR
                     KAPFFNVLAAFWIQFMTHDWFSHLEEGHNQPSWMDVGCQKQLVGNVEQPLSEADIKRL
                     GCRPGDRIDRAYVVEEVEPPTFTHDGQTYLVRAPKTMRNNVTAWWDASQIYGYDDISR
                     KRVKRDGKDPAKLLLEPGKDGGYLPVFTVNDPINPEWSGQEATAFPDNWSIGLSFYHN
                     LFAREHNAFVDAFRKQPGEADSGLRDPARPDEVILYKNVTNDELFEIARLVVSAEIAK
                     IHTTEWTTQLLYGEPLYKGMNANWHGLLRDDSDVSDALKEVVRRLDDSDNPKKSNGLY
                     AALAGGPGIFGLGNRVYEEQSVLSIPFGEKKDVWSLKNPEHVNGGVKHFGSPFNFPEE
                     FITVYRLHPLLPDMIEYREWSADANAIKAKVPVVETFRGRATGAMRQKGLANWTLSMG
                     RQRLGLLTLENHPQFLQNLKMGRLESVTKQIDVPALDIIRDRERGIPRFNEFRRQYGL
                     KQLTSFDDFIDQREKNSQERARQERLINTLREVYGQHPCDESKVITHAQLDDHGKSIT
                     DCLGHSNGTLVDNVEDLDTVVGWLAEFRRPHGFAISETQFLVFVLNASRRLFSDRFFT
                     SSFRPEFYTRFGVGWVMNNGPDVRMEAVPSNGHREPVSPMKRVLLRNVPELAPELESV
                     VNVFDPWARDRGEYYSLDWRPRPDAKTDAAFALQ"
     gene            complement(53219..54187)
                     /locus_tag="VLD60_07980"
     CDS             complement(53219..54187)
                     /locus_tag="VLD60_07980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSB44934.1"
                     /translation="MRDYLEFEKPLREIEEKIEKLAAAGGTKSSTQDDIRKLRTKLAQ
                     LEHELYSNLTPWQRTQLARHPQRPSTLDYIGGIFRDFLEFHGDRSFGDDRAIVGGFAR
                     FNDRSVMVIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMKMAEKFGRPIITFIDTPG
                     AYPGIGAEERGQAEAIARNLFAMSRLTVPIISLVIGEGGSGGALALGVTDRILMLEHG
                     VYSVISPEGCAAILWDDPAKVPDAAAALKMTAQDLIALKIVDEVISEPLGGAHREPKA
                     VTERVAKSLANQLSQLNELSAEQLLAQRDRKYRQIGAVSGIKLSSN"
     gene            complement(54255..>57668)
                     /locus_tag="VLD60_07985"
     CDS             complement(54255..>57668)
                     /locus_tag="VLD60_07985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSB44935.1"
                     /translation="LDGANQIEPLVQQIKAFGQPAVAMTDHGNMFGAIEFYRKAKEAG
                     VKPIIGCEAYMALGSRLAKKDSGLSHNDYYHLILLARNLTGYQNLIKLVSKAYLEGFY
                     YKPRMDKDILKEHHEGLIALSGCLSGEIPYLIGQKDMAGAMAVAGEFQEIFGKDHFYL
                     EVQANGLDHQRVANAGLMEIHKKLGIPLAGTNDCHYLKKEDSRPHELMLCLQTGKTIS
                     DPNRMKFDTDQLYVKSTEEIASAFAEFPDAVTNTCRIADYCDLELALNKTHLPQYKVP
                     DGYTRESYVEHLAIEGLKDRLKDRPSKIPSAVYEQRLREELMVICSMGFAGYFLIVWD
                     IIRFARSRSIPVGPGRGSAAGSLVAYALRITDLDPLVYTLLFERFLNPERVSLPDIDM
                     DFCMDRRGEVINYVVDKYGSDHVAQIITFGTLGAKAAIRDVGRVLEMPYADADKVAKL
                     VPNQLNITLQQALDTEPRLRELVETDMKVNELMTVAQSLEGLARHASTHAAGVVISEE
                     PLTDHVPLYKGANDEIVTQYSMGDVEKIGLVKFDFLGLKTLTMVRRAETLINESHPDE
                     PPLSVDQLPFDDPQTFALLSSGKTTGVFQLESSGMRDLLTGFKPDRFEDIIAIIALYR
                     PGPMDLIPDFIKRKQGKVPITYETPELEPILKDTYGVIVYQEQVMAIANKIAGFSLGQ
                     ADILRRAMGKKKPEEMEKLRVKFLEGAKANKIPEKKAEKLYELIQKFAGYGFNKSHAA
                     AYAVVCYHTGYLKAHYPTEFMAALMTTDMGNQDKIVGYFTECRDLGIKVLGPDVNESQ
                     KNFAVVDGAIRFGLAAIKNVGEGAVESVLAIRAESGPFKSFFDFCRRVDLHKVNKRML
                     EGLIKAGAFDSLGAKRSQLMAVLDQATEDGAAAQRERELGQTSIFGDELNGAGHQASA
                     MEPALPNIPEWDQAQRLKYERELTGFYITAHPLARYEATIHALASATTAGLAELSDGK
                     EVKLCGIIATVKTMLTKKGDRMAYLGLEDLLGTVEVIVFPDLFKTAGDLIAPERLVRI
                     TGTIDRGDKGTKIRGSKIEPLADVQTQTIKRIHIRLSDRPEVMAQLPRLLDVFKRHPG
                     GTSVSLTFRTGGTLEAETAPLPNLSVNASEHFVADVEEVLGKGALSLVS"
BASE COUNT        11709 a        15520 c        17265 g        13174 t
ORIGIN      
        1 ccgatgtcga aaggaggtcg ttatgtcgta ccgctacaac acgaccgaca tcatcgtggg
       61 tgtcggcatg tgtgcgattg tcttcggagc cttactgttc tttgtcgctg cgaacggcac
      121 ctatcaggta gccatcccgc agcccggctt gcttgaagaa ccagccggca tcgatgtcgg
      181 aatgtcctgg ctccagccgg ccctcggcca agccattgtc gaccaggtga tcttcgaacg
      241 ccgcgccaat caggtcatcg cccaatccgc gtccgagtgg aaccgggcga cgcttgctca
      301 ccacgagttc caatcgctat cgggcggtcc cttcggcgcc atcatgcgcc aggctgcgac
      361 agtcccggcc gatcatatgg cccgtgtcca gggcgtcatg gggcgggcga tcgtaaattt
      421 cacgcaacgc ggcattcgaa gtggctcatt gtccgctgat cagtatcttt cagattacaa
      481 cagcggcatg attcgcgcga ctgaagccag agggcaacgt ctcgacgaca atttcgcgtc
      541 cacatggcag gcgactctcg gacgagggat tgtggaggcc gtccaaagct acgccacgaa
      601 agctggcgca atccaggagc gaatggggac ggcgcttttt catctaacgc aggcgcaacg
      661 atcgtcagaa gaagagcggg ctgcacagca ggaacaattc gcgagcctca ttgtggccga
      721 cattcgtacc gaggcgctgg cggatcgctt gacgctgctg gccgccatcc aatcttttcc
      781 ggaagacacg gcagtggcct tcacggaacc agcgtcctgg cctgagattc ctatgggcta
      841 tctgatggtc gccggcttca tgttggcggc agtctttttt ggaggacttt cgctcagcgc
      901 ccggagccgg gaaaccaaag tggtcgatca gatgaagcag accgcaaaca ggtgggttta
      961 tcgtatcgcg gcgtaacagc atgtgagaaa aggactcgcg cggtgcgcgc gcgagtgagt
     1021 gatttatgtc aggcgtctat tggggaatcg tgggaggttt tctggcaatg gtggcgacct
     1081 tgttcgtctg tttcgatctc ctgtacccga aggcgaaggg gtcgcccaaa gcatcgagtg
     1141 gcaggatgga tgagccgggc gaggcaggca cgcaagcctt agacggccac cgtcgggctg
     1201 ctgcatagat gcgagaaaga actaaacgac tctcattata gagacaaggg aggtgcccta
     1261 tggtcgagat catcggtctc gtgacgatgg gtgcgcttgt gtggttgatc gcctggtcga
     1321 tgggagggga aagcgagtct gagaagaggc gtatgtcgat gcctcacgag agtgggcggt
     1381 ccaccgaagt cggctcaagc ggcgcgcgga ccaggcacgc agcctgaacg caacgttgtt
     1441 tggtttccag ctgatacgga aggatggtct tcggaccggg ggttctcttg gccgatgata
     1501 ctgcaggccg cccagcggcc cgcgggaaaa tgccattcac cacgtctatt ccgcgccggg
     1561 ttatggtctt tgctaaatcc aggccagcgt cggccgggtg acaccacgcc cgacgcagcc
     1621 ggaaaggggg atgtatgaaa gcaatgattg gagcagtgct cgtagcagct gtcgtgggat
     1681 gtttcgtcgc gctcggtcca gccatggcag agacagcgtg gattgatgat ctcgccaact
     1741 ctgtctcgtt ctacaagacc aactatcccg cctcgaactg ggagccctac agccagaaat
     1801 tgggggtggt tcgggatgca gtgaaccgtg gagaccaacg aacagtcaaa gtcgagatga
     1861 ccaggtggtt caagatgttg cgcgtccggg accatgggat cagcgatatc gcagcagatg
     1921 agttgttcaa ctattcgttg atggtgacgc cgattcagga gtatgggatt tccgttcccg
     1981 ctccgtccgg gggcgtcggt gaactagcct attgagcagg ggattccgtg ggaggagtgc
     2041 cggcactcct cccacacaag aacgcgcgcg gttgttgtcg aggcagacaa aggaagaagg
     2101 tgacgccatg atgacacgtt ggctattgag cacagttgct atgctggttt ttgtcgttcc
     2161 ggttggcgct gctgagccat ccaaagatat cgtcacgctc gcggcgcatt atggattcag
     2221 cggggtgatt accaagatcg agtccgggat gctgtttatt cataccgaca acagcctgca
     2281 gccaaggacg atcagtccga ataaagcaga ccgcgtggga ctgcacggtg caaaggttgg
     2341 cgagacggtc aatctgctcg ttgattctgg aaatgtgatg atcgatgcgt cgaggacgga
     2401 tcgcttcttc ccggatcacc ggtttgtcgt cggcacggtg cgctataccg atccgtattg
     2461 gcaggaaatt caactatcca ctccagaggg ggccagcacg tatgaggtag atgcgctggc
     2521 agggaataag ttgtccggcc tcccggatgg agccccggta acactggagt tggacgcgga
     2581 taatgtgatg attgacatcc atcgagaccg ctagacctat gcctttgtgg ctgccccacg
     2641 gcttgacttc gttggagggc aacccgtacg atcccgccgt gacacggtgt ccgccggctc
     2701 cttcctgtat ggctggtgga caccggatag ttctccaaac tgataccgcc gatgcgtcgg
     2761 agacgatctt atgccaagtc gtctgcgcat ggccaggacg gtcctgttcc tccttgccgc
     2821 aatcgttgcg acgggagagc ggccagcttg ggcaaaggag gcggcgtcca acaccggagt
     2881 cgcactccag acgattctcg aagtggagca gacggctcgt ttgctggcca ttctgctgga
     2941 ctcgggccgg tccgttgtca atgatcatca agccctctac aatgagccgg ggcaatccga
     3001 taaggggctg accccggaag tctttggtcg acaattaacc gatatgtttc gtagccggtc
     3061 cgggattgat ctggaggatc tcaattcttc caggcttccg gctcagacca aaaagttgat
     3121 gaaggaactc gtagccgtca gccggcatgt cgtagccgat gcccagcctg agatcaaacg
     3181 atcgagcggg ccgggaggct tgcttcccgc gatctatggg gctcgcgtgg ccagtcgctt
     3241 caccgagcgg accggagttc gcatgaaaca aacggcgctc ctgcctcgca atgctgccaa
     3301 tactccggac gcggccgagc ggttcgcgct ggaactcttc gccgacccta attatccgcg
     3361 agaaaagccg atcagcgaga cgaccgccca gagcgggctg cttcgattga tgttcccgct
     3421 ttatgccacc aggcattgtc ttgcttgtca cggggagccg aaaggaacgc tcgaccggac
     3481 cggctatcca cgggaaggat ggaagttggg acaaaatgcc ggagccatca gcgtgatgat
     3541 cccgtttacc aaatgaaaca cgcggcgctg tccatagggt tgctggtgat cgggctggga
     3601 tcagcctggg cggcggagcg tgtcggcgga ccgaccgaaa aaccggcagg cagcggggtg
     3661 atcgtgcatt cggacggcta cgtgctcacc gcgcatcacg tggtggccaa cgcacgacgg
     3721 atcgtcgtgg taacgccggg tgagtttcgt gtgccgggcg tggtggtcag tgccgatcct
     3781 gaacatgatc tcgcactggt gaaagtcgag accgtggggc tttctgacgc gccgctcggc
     3841 tatgccgggt cggtgcaatt ggatcaggaa gtcgtggccg tcgggtttcc ctttggtcta
     3901 cgcgaggtat cggtgacgag ggggcggatt gccgctgtga ggaccaaagg cgtgcagcgc
     3961 gtcttccagg tcgatgctgc ggtcaatccc ggtaacagcg gcgggccggt ctttaatgca
     4021 cggggtgaag tggtggggat tctcacgacg aagttcacac atccgtcagg aatcgtgccg
     4081 gaggggatgg cgttcgtcgt accgatcagc tatgcaaccc cgctgctggc aaacattgaa
     4141 gcgttcgact tcaccacgat cgggaagggc atcaagggcc agaagggagc caatggaaag
     4201 gggttcgccc aggctcttgt tcggactacg gtcaggattg aaacgatgcg ggtgaccgag
     4261 ggagtcccgc cggctacttc agcgacagag tcgggcggaa aaagcgacgt cgctgtcacc
     4321 tcggctgcgc gagggcagac gggaggtctc cgtgaaccct cttccgtcaa ggcggatgat
     4381 gccggggtcc ggcaggtgaa tgagcaactt cggctggttc agcgagagga attgcaacga
     4441 cttgccgagc gggggattgt tcctcctgaa gggatgatcc tgattcccgc gggagagttt
     4501 ttgatgggat cggatgacgc gttgccggac gcgcgtccgc tgcatcgggt atatgtcagc
     4561 gggttttggc tcgacaagta tgaagtgacg aaccgtcaat accggcaatg tgcggaaggc
     4621 ggcggctgtg ccgttcccaa ggatcgatcg gcctatgacg aggcgactcg ctcagagcat
     4681 ccggtgacga acgtaacctg gaaccaggcg cgaggctact gccggtggct gggcaagcgg
     4741 ctaccgaccg aagcagagtg ggagaaagcg gcgcgcggga ccgacgggcg actctatccc
     4801 tggggcaata atgacgacgt gctgcgagtt cgtgtgcggg gccttgaaca gaagctcgtc
     4861 aagaatggga ctgagcctgt cggcagcatc gatttcgccg tgtccccgta cggagtggct
     4921 gatcttgtct tgaatgtgtc ggagtgggtc aacgattggt acgccgagga tttttatcgc
     4981 tcgtcgcctg cccgggatcc gcaaggccct gtgcgcggct ccttccgtgt gctgcggggc
     5041 ggggaagtca gcgaccgacc gttggaattg cgcgccagct accgagggtg ggatgacgtg
     5101 acctattggg ggccctcact gggatttcgt tgcgcggacg atctaccctg agcgacgcgc
     5161 ttattttccg tgaccgctac tggcgatcgt ctttcgttcg ttccgctcaa ctgaacagaa
     5221 taagccttct gattacaggt tgttcggcgt ctgacatgaa gttttaggtg agtatctgcg
     5281 gttgcccgat cccggcaatg acctcctccg tgatcaccac agatttcact ccgccatgcg
     5341 cagggatctc atacatgagg tctagcatca cttcttccag gattgtgcgg agagcccgtg
     5401 cccctgtttt catcaaggcc gcgcggcgag cggcagcctt cacggcggag tcggtaaact
     5461 tgagatcgac cccttcgatc tcaaagaggg cctgatactg tttcaccagc gcgttgcggg
     5521 gctcggtgag gacgcgaatg agtgcaggga catccaggtc ggatagcgtc gtgaggacag
     5581 ggaaccggcc tacaaattcc gggatgagcc cgaatttcag cagatcctct gatcgtacgt
     5641 ggcgaagcaa gtcggtgtgt ccatcgatcc cgctcgtcgg atcgctcgcg ccgaatccca
     5701 gccgcttagg catcgtccgt tggccaataa tctgatccaa ccccacaaag gcacctccgg
     5761 cgatgaacag aatgttcgtc gtgtccacac gaatgtagtc ttgttctgga tgtttccgcc
     5821 cgcctttcgg cggcacattg cagaccgtcc cttccaccag cttgaggagc gcttgttgga
     5881 cgccttcgcc tgacacgtct cgcgtgaggg acgggttctc cgacttccgg ctgatcttgt
     5941 cgatttcatc gatgtagatg atgccggtct cggcgcgctt gacgtcgtaa tcacagttct
     6001 ggagcaactt gagtacgaca gtctcgacat cctcgcccac gtaacccgcc tccgtcagtg
     6061 ccgtggcatc agcgatggta aagggcacat gaaggatgcg cgccagagtc tgagacagaa
     6121 gcgttttgcc cgtccccgtc gacccgatca tcaggatgtt gcctttttgg aggtcgacgt
     6181 gtttgagccg gtcccggttc agaatgcgct tgtagtggtt gtggaccgcg acggagagga
     6241 ctttcttggc gcgatcctgc ccgatcacgt agttatcgag tgtcgctttg atttcagtgg
     6301 gcttgggaac caccagcgga acggaggtgg aagaaccctg cgctccattg tctttcttca
     6361 gggacgcgga gcactgttga aggcactcgt cgcagatcag aagcggagat gtcgtgcgac
     6421 aggaattgca ggtgtagggc gcaggactcg cgatgaggtt ccgaacggcc ccctgacgtt
     6481 taccgcagaa tgaacagaac ggatcaggtg gtgatgaagg ttgccgcgtt tcccagaaca
     6541 cagccgcctc cttcgtttga acaggtcagg ggacggtgac ggggtcatct tatgcctagc
     6601 cgacagtgaa cgcaaggggg tcacaatgcg aactcaggtg taatagtcct catggggaga
     6661 gaggggattt gaatgtctga agcgattaac cattgcgttc cggccgccat tgaaggccta
     6721 gtgtgatgat ctgctggagc agctttgtat acggcaggcc agcccgtttc gcggattcgg
     6781 cgaaatcttc gtcctgggcg atttggggat tcggattcgc ctccagtaca tagatctttc
     6841 cctctttatc cagccgtaaa tcgatccgcg catacccgct cagttccaac gcacggtaga
     6901 ctcgtttaca gagatgctgg atgcgctcgc tggcgccttc gggcgcgtct ttgagttcgg
     6961 ctgtctggat cccgtatttc tcctggtact tgatgctcca tttcacccgc tccgtggcga
     7021 tccgatgcac gtcgtccggc atcttggtaa attgcatttc ccacacggga aagatccgca
     7081 gtcgctgatt acccatcacg ccgacgtaga gctcccggcc gtcgatgaag tgttcgacga
     7141 gcgcatccgt cccgacgctc tcgtggatga atccgacccg ttcaatcagc ttctgctcat
     7201 cctcgacgac cgaagcctga gagatgccga gcgaggattc ctccgtcagc gatttgacga
     7261 tcatcgggaa ggtgaggcgc ttcggcacgc ggatcttctg tcctcgccgg aagacggcga
     7321 actcaggcat cggaatgcgg tggtacgcca tgagtttctt ggagagtgcc ttatctttgg
     7381 ccaggagcat gccccgcgga ttacaaccgg tatagggaac gcgcagcaat tccaggtagc
     7441 tcaccacgtt cggctcgaag aggctgacat tgtgaaacgc ttccagcaga ttgaagacga
     7501 tatggggctt ccaggtatcg atggtgttgc ggatgatgcc gagatctttt tccaccccca
     7561 acggctctgt ctcatggccg atctttcgca gcgtcgtcac gacatcaaac tcggtcttcc
     7621 attcggcctt ctgcgtctcg atgcctgtcg tatcctgagg cggcaccaaa tgcttgtgca
     7681 tgagcaccag gactctgagc ggtttcatag cgccacccgc cgtccgccgg tgttgacgta
     7741 gttcatcgct tgcaccgtca agaagactgt aaactcttgt ttagcctgct cctccggcac
     7801 tgccaggcga aggttgagcg ggcggcagtg ggcgatcagg tcttcgaaga cttgatcaat
     7861 cgtgtattgg tacacacccg tccagccggc gacgaggcgg cgaaccggct tccgaatctt
     7921 catgaggaaa cgcacagctg tcatgttgcc ggcgtgctcc gccgcatcgg aaaagaagcg
     7981 ccgcagatca cggtcgttga aatccggatg atcgagaccg taatgccggc gcttcttctt
     8041 gtaatgccgg cgcagggtcg tgcgcagccg ccggagcgga tcgacttcct cggtggagac
     8101 gttgagcgga ggccggccta ccagggtctg catcagctga tcgacgtatt gaagtttctt
     8161 gagcgcgggc catccgacat agcgtgtctc ccactgagag ttcggcgtca gccacacggc
     8221 gaaggtttcg gcgaaatcct catcaggatg gctttgtgcg taccacgagt cgagatgcag
     8281 cacaaagctc ttgctgtagg gcttgggcga atagtcatcg ggatacgcct ctgagctctt
     8341 tccgaagagt tgctgccgat gccgccgccg ccgcagcctg aaggcgttct cgatggcatg
     8401 accggtctca tgccgcagga tgcgcatgca ccagtctgga tcgccccctt cgacttccag
     8461 catctgggtc aattcgagtt tggcgagccg ggggtgggcc agatagaacg gaattgcgat
     8521 tcctggcaca tcatccgggc agaaccattc atccgacagc caaaaatggg gatgaaagtg
     8581 aaggccgcgg ccatccagtt cacggtaaag ctgtgcgatg cgatcttcaa gatcgctgcc
     8641 tgcaattttg agatcgaggt cgcacagccg aagatccagc aacttctcat ccggccacgt
     8701 cgcccaatcg gtgagatcgc tccgtatggt atcggaggat gcatcttcta ccggctcgct
     8761 cttgtcttga gttgccatcg ttcaggtaaa cgtcacatgg ctgaagaagc aggacattgt
     8821 acgttttgga aggcagtcca gtcgatcccc cactgcggga aaagaaagtg gtcgcaggaa
     8881 ccggtttgat ctgaacctcc gcggcagcct taattgaaga acctcctccg cgcgcggcgg
     8941 ttgtcgcaaa tgtcttgcta tgcgtcgatg tggaaagaaa cggggtcggg ataaactagc
     9001 ccgagtagcg gcctccatca agacgactcc gttctccaca gagatgaaaa acgccgttga
     9061 caagaatccc cccctttatt ttaatctgcc agcgtcgagt gataaggaag ccgtgacaca
     9121 agggagtggt tcacgctcac atacccgttg atcacatgta gatggtgcga tcgctaaggc
     9181 cttatggcga gacgatgtac taaaaagaca gtcgcgcgga tgcctcttta tattgcaatc
     9241 ctgatagggg cgctgggcgt tggatcactc tatgccgaag aagaaatcat catactgggc
     9301 aaaggcaaga cacaggctct tgcggaatgg gacaaaccgg tccacttcgg gacgggcgat
     9361 tggaaagttg tcgatgacgc acagcatcag gccatcaagt tacggaccga atcgtcttcg
     9421 ttcgccttgg aaaaaagcat cacggtggat ttgaaacaga cgccatatct cgaatgggag
     9481 tggaaggtga ctgtcccgcc ggccggaggg gatttttctt ctcccgacac ggatgaccag
     9541 gccgctcaat tgctcgtcgt ctttcccaag acgttcttcg agcgtcggaa agttatcagc
     9601 tacatctggg acccgactgc gccacaaggg acaatcggaa ccgccgccgg tccgatttac
     9661 ctgaatgtga aggccattgt ggtcgaatcg ggagccggtc agatcggcaa ctgggtaacc
     9721 gaaaagcgca acgtcgtcga agattatcgg gcgctctttg gggaaatgcc cgaacgcgct
     9781 gtcgccatcc gtatgcagat caacagtcag catacaagat cagttgccga agtgttctgg
     9841 aggacgattc gattcacttc tgagtgagat ccggccatga aaaccatatt gtacaccccc
     9901 agtagaatcc cgcaaataat gagatttctt ctggcgagtg gactgctggg ttgcttgatc
     9961 gcaaccgcgg ctcaggcgga gtggtatgtc ggtgggtatg gaggcatttc cattcctggc
    10021 gggctctcga atgtcaccgt ctccaattcg accctggggg gcggcgtcac tgacgccaaa
    10081 atcaatgatc tcgaactcaa gtctaatctc ctttgggggg cagggcgggc tatttccttg
    10141 agacgcgtcc ttggctggga ttagaagcag atctgtacac ccagaaaccg gatgtgaagc
    10201 aacagacgat tgtgggaggg acgtcatcag gcaacgtgtt tgccactagc atccaggaga
    10261 cctcacttcg ggttactgtc ttggctgcca acattgtcat ccgctcgccg tccatgagcg
    10321 aggtctttca accctatggc ggcgtcgggt atggattgtt ttttgctacc ggctcacaga
    10381 atggacaatc gaacacccaa attagtcctg gatttcagtt cgacgcaggc gcccggtatg
    10441 tgttgacacc ggaatgggcg ttgtttggcg aattcaagta taaccacgcg acgatcaggt
    10501 ttagcgggat caaaggcaat tacgactccc aaaattttaa cgtggggctg atgtatcatt
    10561 ttgggaggta gctctcccgt cagacttgtg tcgactggct cctcgcgacc gtgattactg
    10621 agggcgtaga ctgggtccgg cctccggacc gacgtacttg tagcccttat gctccatgca
    10681 ctctttcaga agggggatgt ggatccagta gatcaaattg tctgtgtaga tttcctctct
    10741 tttctcagat tctgcatact tcacggtgga ccatctcgta cagacactgt aatccgtcaa
    10801 aacctgcgag tacggcacct ccttggcttt ccaatacgtt ggggagctag ggacaggttc
    10861 cacgggaatg catccagaca tccccaatac gacgcaccac cacaccatcg cacccttccg
    10921 gacaaaggca ggtttcgttg tcatgatctc tcctccacct ctttcaccgc agttgtcaca
    10981 ggggcctcct catactccga aagccagccg gaggcgaacc cgtgagccca tcctctgtat
    11041 accgccgttc aaattaagtc aataaagggc aaggcatttc gtgcgcgaga ggtgagggaa
    11101 aagggggtcg ggagtttttt gttaatctcc tcgtccgcgc gcggcgatta tcggagggcg
    11161 tggacatgaa tctgtcggtg gcggtacatc gacaaggctc gttgttctga ctcttcggct
    11221 tcggtcatgc gtcccatctt ccggtacagg tcggccaaga gagacgtcgc ggctgcttcg
    11281 atctcggcgt catgcagggt ttgggagaga aagaccgcct gcttagcggc ttcgacgcct
    11341 tcactcagtt gcccatcttc gccttcgaaa taggcaagcc tgagctggag cgcggcttcg
    11401 acaggcatgt ttcgttccgc ctcggccaac cggagagcgg cttcgtaaaa ccggcgcgca
    11461 tcgtgtgggt gatctaacag ccaatgcgcg gttcccagcg ccatgagtgc ggcgagatgc
    11521 tggctgtgat cttctctggt gcgcgcgtca tcccgtgcct gctccagcaa cgacagacct
    11581 tgctcgggga agtcgtcact tacttgaaga aggcccagtt gaatcgatgc ttcattccat
    11641 ccccggcgat ctttgagtcg gttgaacagc gcgattgctt ggctgaggga ggtatgagcc
    11701 tcctgctgat aaccaagccg agcggagatt cgccccatca cggagaggct ctgtgcatag
    11761 gccatccggt cggaggattt cgcgagtagg cgtacggctt cctggacggc tgtgtgggcg
    11821 tcgtggattt tgccttggcg ttcatagact cgtgcgattt ttacaagggc tgtcgcgatg
    11881 ccgcgagtct gcttcttttc tcgaaacgtc gacaatgcca gttcgtagta cgtcagtgtt
    11941 tcgcggaagt ggagttgatg atcgtgaatc tcaccgatcc ggagcaattc atcgcctgtg
    12001 agcgtgctgt tcgaggacac tggctgaacg gacgcagagt ggggaagcga ccaggccgga
    12061 ttctggtcca gcaggagaag gaaggggagc aagagccaca tagcaggcac gatcagccct
    12121 cgccatgaga gtagatccgg aatcctcggc gagattccct tactggagca ctgttaaagc
    12181 agtgaatctg ccgagtggat cattgaaagc atgcgctccg cggacagaga ggtcaagttg
    12241 gcatactctg ggaataggag atgctgcagt cttcgggata gagcctggaa ctccggtccg
    12301 ggcggggtcc tggtttcctt gaccgcccag gtgtagaatt caacaaagtt ccattcgaag
    12361 ctcgatgccg ccagcctttt cgtgtgacat cccaccttca accaggaggt gtggtcatgt
    12421 tctctgcatg ggatttcaac aagtggatga tcagtgcggg aatcgtgctg cttctggcca
    12481 gcctcattgc gttcttcgcc gcggttgtca ccgagatcat tattcggatc tggggagaag
    12541 agcgcggcgt ggtgccgccg cttcgcatga gtgaggccga gcagaaagcc ccatcccgca
    12601 aagccgcttg aggttgttgt ccggaagtga aggagagggg tcgtgggtct tttgttgaac
    12661 tcttccttcg cgcgcggcgg gtctgtgtaa tcttcaacgc cgtatctatt ttctgttgcc
    12721 cctgcttgac actttttcgt gaccggccta gccttttgtc attctctcgc accggatcct
    12781 aaccaggagg ttgaccgtgg ggaagatcaa ctgggagaaa gtgtttctcg gagggctcgt
    12841 cgccgggatc atcatcgacg ttgtcgaggg gattctggag ggggtgatcc tggggcccga
    12901 gtggcggcaa gcgatgcagg ccttggggca cccgctccaa gaaacggggg tcaacatgac
    12961 gctgcatatc ctgctgggat ttgcctacgg gctcacggcg ctgtggctgt atgcggcgat
    13021 tcgtccacgc ttcggctccg ggccaaaaac ggccttctat gcgggagttg gtgtctgggt
    13081 gctcggcagt cttcttccat cggtgaactg gggcccgcgc ggattgctcc ccggccacct
    13141 ctttaccatc gcggtcatcg taggactcgt cgagatcgtg gttgccacgg aagcgggcgc
    13201 ctggctgtac aaagagtagg ggacggatcc agaaacgcag ggcgtttgcc tctctgcttt
    13261 cctcggtcgt gcgcagcatt gatcgcctga tccggtatca tgtctacgcc ctgcggtatc
    13321 cgtcactcct tctcgcttgg tccactgctg ccacgacctt ctgtttccga aaaggaggcg
    13381 cgcgcttatt tcttggaaca gcgcgggtca gctgcctgca attctgcgat cttttgatcg
    13441 tccaacggca ccggcgcaca gccgtgggtc ctttcgcaga cgaagtcgta cttgatcgcc
    13501 ggctggtgac agctgaagca cttcaccccc tggaaattca ccgtctgctc tccacgagtc
    13561 ttgatcttcg tgccttgggc cgagaggtcc agatcaaaaa attcccagcc gttcgtttcg
    13621 gggaattttt ggcgtgggtg cttcaccatc gcctcgttcg gcagaagctg gaggaatgtg
    13681 ccgaccggat actccttatc cgacacgctg ttcttgaaga tacgaacggc ttctttgagc
    13741 ttctcgggat ccgcatgcgt gatgtaggtg tttctgactt tggtccagtc gcgaatgcag
    13801 ccgaacgagt tttcgcccac cgtgagatcc gcagcataga ggctggaaga catgaagagt
    13861 cccaacgcga ccaccacgac gcccctcatg cctcccagac ccgatatctg cgatacgtat
    13921 gtgatgagac ggcgcatgcg agtgctccct tctcctggac gtgaaccgca cggccggctg
    13981 aaccggccgg tatgctttgg agtattgtag tcgcccaggg atcgagattc acgtggtact
    14041 ttgccggaag ataagaaagg tgtaaggaat cccttgtgcc tcttccgtgc gcgtaccctc
    14101 ggcgggtaat gtccagccgc tcattgaagg ctttaagcgg atttcatatt gcgagcggtc
    14161 aggacgtgag cgtgcgcgca tccgttagtt ggtattgtcc tcgtgttatc gtgggataga
    14221 cggagaagat cggggtattg gcagggacga cgccgcttgt cacgttcggg agcagttgga
    14281 ggtgcatgtt gatgaaattg aagctgagcc cttgttgcca agatcgactt ccggcgccgg
    14341 cgtaagccgc ggtcttttcc gaagcatagg tcctggtgag gagcccggca gcgcctccca
    14401 agccgtattc ataaaggtcc ggtgcgcctt gaacgagcag ggccattact gccgcgtcga
    14461 accacacttt aatattgcag ccgatttgca cgtagggtcc gagggtattg gtgccttcga
    14521 gaaacgcacg aggcagaccg atttcaaacg ggacgtccga agctaccgcc cgtgtgacgg
    14581 tgcccaggtt ctgctccttc aagggagaaa ctgacacgaa cagcaaatgc gggtcggcca
    14641 tatcgatgtc gagaacctga tcgtcgtagt agctgcagag ccggccgagt tcatagcgga
    14701 ggggaaagga gtaacccatc tttgcgtacc agatgcgcag accggcatcc aacggatcat
    14761 ccggcgtccg gctgatcttg aggtcgaacg gcagcatgat atgccaggca ccggtcctcg
    14821 cggagacaaa cggcgcacag gcaccggggt attctttatg caccgttgtc gtgtcgggtt
    14881 cgaaccgtcg cgtcggtgag gagaaatgct gggcgatttt ggcttctttt gggaggcggt
    14941 acttttcttg gtagtaagca gaggctcgct gttgcgcgat tgagaaaaag tagtccggca
    15001 tatcggtttc gatgatgatc tgtcggtcgg caaactccgg tcctgggcca gcgcggtcgg
    15061 gcttcatcgc cgctgtataa aaatccaatt cgatcgtcag gcgttggatc tcctggcgga
    15121 tatcccgcgc caggcctggg tcgaaaaaac tggtttgggg caagatgtcg gcggccttct
    15181 gcaggcaatc taatttgtgg gtgaatcgtt ttggttcatc ggcaaaggac tcggcaaccg
    15241 ccatcaggag attgaactgt tcttccggtg tcaggccgag agtttggaag ttgcgatcct
    15301 tcacgaacgt gaaggccatt gggccgaagg actccgcctt gcggatgcaa agggtgagga
    15361 gaaagttgta atgggcacgc gcctcttctg gtgtcatgag caagcccctc cgtggcattg
    15421 taccgatgtt agatttagag gggcaaggtt ggatgtcaca gtagaaacaa gatcaaaggt
    15481 tcggttcggt atccaacgtt gagagtttcc tccgcgcgcg atgggaccgt taaccaggaa
    15541 tgcgacggag ttctggacgg gcgacggcga ccgtgtcggt aaagagaagt gacggtccga
    15601 gcggcagaat ggcctggccg gtgaaagcga acgagagact tccgatcgcg tgatagaacg
    15661 ccagtatgcc gatggccaag cgtcctattc ccgggagggt ctcggagatt catcccggcc
    15721 tcgcgacgac cgtcgcgaga aacgtcgagt catcatgaag taattttgaa ccgacgcata
    15781 cggtgggtta gtaattccgc tggtaacggt actgcatcag gataccttcg gctcggcgtt
    15841 gagcgcgatc catccgctcc acattgtatt tgtgaatcac atccctggcg atgccgacgg
    15901 ctatgatgtc gcttgcgccg agcttcaggt attcatcggc gatgtccatc aattcagaag
    15961 cacgatcttc aagggatgat ctgaatccct tggggaaatc ggaagaggac gcatcgagga
    16021 ctcgatagag aaggaacccg tgatcgaggc aggctcgtac agctgtttca tagggtctga
    16081 gggcatcgag tgttttaatg aggcggtatt ccgtggcggc ggcctctgct tcccgtgcga
    16141 gttgctgttc ggtgctcttc cattcagcgg agccttgggg agactggagg tactctgcga
    16201 gcggccctgt gctctgttcg cccagggcgc atgctgtcat tgcccacaga gaggcaaaca
    16261 tcaacaactg aattgcgcgc gggcagagag acatgtgatc tccttcgttt gagttaggtg
    16321 attatagccg aatctcgttg attccctcgc agtttgtttt cggaccattg aagctggcgc
    16381 gtgcgcttac aacgaagatc cggaccaaaa tttaagcatc ccggtggctt caagtatcgg
    16441 actacggcta ggagaagcaa gggagactag gggggtgagg acaggcgagg tgacgctggg
    16501 tctatgggat agatcgcatg agccgggtcc tgtctattca gatagtcacg gacaccgtcg
    16561 atgggtggct ggcttggttt cgcaatggag tccagctcgt aagggtcgct cttggagctt
    16621 ggtttcggca atggctctct cgttggttcc tcgctgctaa acccgagcca tcccttcagc
    16681 gcctggaaca gcggtttgct gctttctctg ggactctcac aggaggtatc gccacgttct
    16741 tgttgcagtg gggtgcatgg gctgagctga gcctgagtga cggtgaccgg tgatccaaaa
    16801 atcagcagcc caatgatcat cgcgacctta ctcagtggag ggtgtctcgt caccagcttc
    16861 gtacaagggc gcgtcatgat ccgtgtgttt atcaacacta ttattttaca cttctgacat
    16921 gctccgctaa accggcccag gagagtgaag cggagtcaag aaccgtttcc acttcggccg
    16981 cgcgcggtgg gtcaatgcgc acctcgtcaa tgtcacgctc ctatagttga ctccatttac
    17041 gaccccaagt agcctgtctc gaattttctg ctcccaaccg ttcgggcaac ttgaggaggg
    17101 gtgaaggatg gaagggctgc atcaccttga gatcgtcatc ctcctgctcg cgctggtgtt
    17161 gacgctgacg acggtcgccc aaaagatctt cattccctat cccatacttc tcgtgatcgg
    17221 tggattgatc ctgagcgtca ttccggggct tccggcggtc acgctcagtc ccgatcttgt
    17281 gttcctggtc tttctgccgc ccatcctgtg ggccgccgcg tatttcacct cgtggcgtga
    17341 gtttcgcggc aacctcaggc cgatctcatt actggcggtc gggctcgtcc tggcgacgac
    17401 ggctgcggtt gcagcggtga ctcacgcgat gttgcctggg atcggatggg ctgaggccat
    17461 cgcgttgggc gccattgtct ctccgccgga tgccgtgtcg gcaacggcga tcggacgacg
    17521 actgcgcatt cctcgtcgga tcgtgacgat cctggaaggg gaaagcttgg tgaacgatgc
    17581 aacggcactg gtgctgtacc gcgccgccat cggcgtcgcg gtcggcggga gctttgtgct
    17641 gggcgagacg ctgctccagt ttatctttgc cgcagtcgtg ggtgtggccc ttggtcttgc
    17701 cgtaggcatg gctgcacgct gggcactctg tgccacagag gacagtttta ctcagactgc
    17761 gatcaccttg ttggcaccgt acgtagcctg ggtactgggc gaattgacgc aggcatccgc
    17821 ggtgctcgct tgtgtcacgg gaggcttaca tgtacggcaa cattttagtg cggccgtggc
    17881 gccgaccaca cggctgcagg ctcgtgcggt atgggatctg cttgtgttcg tgttgaacgg
    17941 tttcattttt atccttattg gcctgcagct tcgtgtgttg cgcgaggcgg tgccgtccgg
    18001 tcggttcggg cagctcctga tggccggtgc gctggtgtgc gtgacggcca tcgtcgtccg
    18061 tttggggtgg gtgcccttgg ctgcgatact gcccagggcg ctgagccctt cgttgcgggc
    18121 gcgcgacccc atgccgcgct ggtcttctct ttttctgatc ggctggaccg ggatgcgagg
    18181 gatcgtcacg ttggcggctg cgttggcgtt gccgttcacg accgctggcg gacagccttt
    18241 tccctttcga gcggaaatca tcctgatcag cttttcagtg attctggcca cgctggtgtt
    18301 gcaagggctc tcgctgaccc cgttgattcg ggttatgaag ctccaagaag accgcgggca
    18361 ggagcaggaa gaacgtcagg cgcgcgagca tgcagccacg gtggctttac gacgattaga
    18421 tgaattggca ggggaggagt ggttgaacgc ggattatgtc gatcgcctgc gtgtccatta
    18481 cggccggcaa ctgcaacgtt tcgcgagttc cggtaccgtt gacccggact gttctctgga
    18541 ggcaggcgac acctttcgac ggttgcggca cgagacctta tcggctgagc gcctggctct
    18601 catcgctctg cgcaatgacg gaacgatcag tgatgagctg cttcatcgtc tggagcacga
    18661 gctggatgtc gaagcgttgc gtcttgggat cggagaactc cgcgtctcga gatgatggtc
    18721 ggtgggaaaa gaaaatcagt cgggagtcat ctttgatgag cctcgttcgc acgcagcggt
    18781 tcccgttcac ccgcgagtac ctctgataat cccgtttctc cgctgatcct tgacgccttc
    18841 ccagtccgag cgtaagtatt tcctcatgaa gaaagactcc tggctctatg gggtcgcgct
    18901 tgtgtccggc gtggtggttt ggattctggt ttcctccttc tccggtaagc gagaggcctg
    18961 ggactcagat ttatatttca ccttcgggat tccagtcctc tgcctcgttg ccgggctgct
    19021 tggatatgtc gagccgaatc gaccgtggag atgggggatt gatcccctct tcgggcaagg
    19081 tgtctggatg ttggtcagcc aagggtttgg caatctgttc cctctcgggt tattggtgtt
    19141 gggtctcttc gcggtcccct cgatcgtgac cgcgaaggtt ggcgctgcga tctcaaagcg
    19201 agtcacgaga ggcagtaatt tccattgatc cgttcaccgg ccttgtccac gcgtggcggg
    19261 gtgtttcttc atcgcccaaa cttctctcca ggtctgttga gcttggcctt ctgatggggt
    19321 atcgtttcgg aaagaattcg gggatcttcc atgcaaggac aatcggacac gctcattcga
    19381 gaccggcttg ctcgtcaacg gactgagttg gcgaacgagc gcacgttgct gtcctacatc
    19441 aggaccgcgc tgggtttttt catcgtcggc attccggctg tgtggtggct tgagttgccc
    19501 ggtattcaag ccctgggtgt ggtgtcgttg ctggcaggtg tcgggttctt gggagtcggg
    19561 gtttggcggt tctactctat caaagcagtg atcgatcaac agccggacta aatgcgcgga
    19621 ttctcgactc gaggcctcca cgaaatccgt ggcagggttc aaagtgcatt cagttgttat
    19681 ggcacacagc ggtggagcaa agatgcagag gaactggata gtgagagtgg taacggtcgg
    19741 gcttgtgagc ggtcttctat tcgggactgt ggcgcaagga caagctgagt ccaaggccaa
    19801 gagcggcgat gtggcgcgtg gcaagaccct gtttgtcagg aactgcgccg gttgtcacgg
    19861 gtctcagggt aaaggagatg ggtataggat ccttggcgcg gatccggcag acctcacatc
    19921 accatcgatc acaaagaaat ccgatgccga cctgctcaag acgatccatg acggcaaacc
    19981 gaacatgccg gcttggaatg tccacctctc ggaggaccag agcagagacg tgctggccta
    20041 tgtgcggacc ttcgccaagt gaagcgatgc aatcggcatc gttgccgaca gggctgtcgg
    20101 ggagaagaaa agggaacagg agtctttgtt gaagtacctc ccccgcgcat ggcaggtatt
    20161 tcggatgcgg tcgacgcatg actccggggc gctttttccc tgacgcctca atccgccagt
    20221 aactttcctg tgcttaacgt gttcaacttg atcagctcat ttatcgtctc acctcttgaa
    20281 tggttcagcc tcactttgaa gcttgtcgta gaggctcact tccgtccccc tcagccttgc
    20341 cgagaaagtg aatcggttcc tcacctctga gatgggctgg tcgcaggatg tatgttccgt
    20401 tgatgctcgt ggtccaaacg gattgtgcag tttgcacgtt ccctccagaa aacagatagc
    20461 tcagtccacc gacaacggcc cctgagatgg cgaatgcgct tttgccgatg caatagggaa
    20521 ccgtgagcag ccaactttgc acttgtatgc cggtgccttc ggaatcgttc gtgccgccca
    20581 tctgatcgtc ggccagtgct gaccctggtg cgatcatggt caaccccatc aagatcgtga
    20641 ttagcacgac ggccaccgat ctgctccatc tcgcgatgag aggagtcgtc cggccgcaaa
    20701 tgggagatat tgggagtctg cctcctggca cttgtcgttc gtccatggcg cgcctcctga
    20761 aagaatgatc atgagggatt gtcgactcgc aagattctaa tcttcacgat gaggggtttc
    20821 ccggccagcg gatgattgta atccatcacc gcggcggtgt ctgacaactg tgccaccgtt
    20881 gcctgagagc cggcgcgatc ctgaaggaca tcgccttcct tcgtttgagg cggcagttca
    20941 tccctcggta ccgttttctt tttcctgcca tcatacggcc cgaaggcctc ctccacggaa
    21001 agctccacat tcttttcgtc cccgctcttc atgccggtca ccactcgttc caacgctgga
    21061 agaagttggt gttgcccctg aacgaactta ccgatgtccc ggacgatgaa cccttgttcg
    21121 cctggaatgg taatttggaa catgagggtg acattcgatc catcgatgat ccgcgggctt
    21181 cccgcggact tttccgtagc caggctttgc cctgagagaa gtcccaggca tatgaggacg
    21241 gtgattaggg ccacgatggc aatgttgttc cggtgagtgt tcttcatatt tccctccttt
    21301 ttccatcctt gaacagtccg ggtatttccc ggtgcacgac tatccggcca gcttgatgat
    21361 attgctaatg cggtcattcc gatcgagttc gatcgagacc tgatcacccg tggagagttg
    21421 tttggtcgtc aaaatcgtgg gatccattat ggggagcgtc caggactgcc cttcctgcgt
    21481 caggattgtg agggtccgtc tggtctcatc gatgcctacg attgtgcttc ttctatctat
    21541 aggtggtcgt cgctctactg gtgagggcgt tcacgcctaa cagcaggcaa aggagactga
    21601 tggccgcgat cgtgagctga tactattcca tgcttgacct cagactgtta agaacggaat
    21661 tgcggagagg gcaccgtgtt gaggtgagtc cctcctgctg ttctgtaacg tggtcctggc
    21721 cgtcccactc ggtttatcgc ccgcaagtgg tttgccaaaa gacatcgaag agagaaaacg
    21781 gtcaatattg caaagactta gagtttcctg gctcacggtg agggtgagga ggacttatgg
    21841 ctcaaagaac atattgtcct gcgatctttc gaaaagagtt gaatttctgc aaggatgtga
    21901 tcgatgacat tctggattct tcggacaact tttcctgact tccacaggag gcaaccgatc
    21961 ggaactggtt gaaataccga cgcgtacgag attggtactg ttcatgcgtt cgtactcgac
    22021 ggcaggttcg cccattcttt cacatgaaag aaggcgcgca tcatgtggtt cgctggcttg
    22081 atcatgagca tgctcttgct cggatgcgcc ggtttcgacc tggaagagcc ggtggatctg
    22141 agcgaattgt cggagactcg atccgacgat gagggcgaga aaaatttggc ggcgattcgg
    22201 accatgatag caaaggagca ccaacgaacg tcgcgtcatc cagaatcgcc agaatcgggt
    22261 gacaggccga aggttgaagg ggagtcgatt ccatggccgc ccgactggct gtcacaatac
    22321 ttctcgtcga ggcctttgtc agatgaggag tccgctctca tgtctgaatc ttcggtgaga
    22381 acaacgccgt ctcgttcgac gcgtcggcca gtctccggca atatcacagc aaagaccccc
    22441 tgggtcccga agtcgtcttt tcgtggtact gaagctgatc cttggccgca tgttcctccg
    22501 tatatacttt ctgctccagc cgggtcgacc tatccgggaa ccatgcggtg tgcgccggat
    22561 acgatcggga tcggcggtca acggtgtcag gccaactgaa cacgcaaatg gaagaattcg
    22621 caccgcgtga agcctcttct tcaatgagga aagagaaggg tgatcgtcaa cgaagagggt
    22681 tgatggcgcg gatcatatca tgggagccgg tgaagacctt gcaagtccgc gtgcttcgga
    22741 tcttcagaaa ggaggacgcg atgacacgac cagtaaaacc ggtcccggcg ggctatcata
    22801 ccgtcacccc tacgctcacc attcggggcg tcgacaaggc cctcgacttt tacaagctcg
    22861 cattcggggc agaggagcgg atgcgtttct tcgcaccgga tgagaaaact attatgcacg
    22921 cggaactgat gatcgggaat tcgatcatca tgctgaatga ggaatatccg ggcatgggat
    22981 gtcgtggacc gcaagccctt ggaggtacct ccgttggtct ctatctctat gttcaggatg
    23041 cggaccatct cttcaaccgg gcagtgtctg ccggtgcaaa agtggatatg ccggtcacga
    23101 atatgttctg gggcgatcgc tgcgggcagg tgaccgaccc gttcggccac aagtggaccc
    23161 tggcgaccca taaagaagac ctttcggccc aggaaatgcg gaaacgtgca gaggcgtttt
    23221 ttgctcaaat ggctctggag accagatagc agttcgtgga agacatgcct acaacgtgaa
    23281 cttgtaggta cggagaacgg gaaacagggc agtgtcatct cctgatcgcc tcgtctgttc
    23341 gcggcgattg ttttgccggt gtactccaag gaaactcaat gattggcgga aagcagcagt
    23401 gacgggaact gggttggttc tcatgctgct cgctcgcgga ttctctttga gcctgttatc
    23461 ggatatatgc cacagcgtcc ggaggacatc gctatgacca cgctgacaaa tcgcttcagc
    23521 tctcttggcc gagtctggtg ctcacgtgtg ccgggattcg tgctctgtgg ggctctcgcg
    23581 ctctcctgtt ccggttgtct ttcgttggct gtgggcgcag cggggggtgc cgccggcgct
    23641 gtgtatgtga tgggtaagtt gaaagacgag cagagctatg agatgtccgt tgtgcacaag
    23701 gcgaccatcg ccgccctgga cgaacttcaa ttgaaggcct tggaagacaa agttgataaa
    23761 ctctcagcgc atgtagaatc agagtttgcc gatggggagc acgtgtggat cgacctggaa
    23821 tccctctcgg attcccgaac cggtctgacg attcgcgttg gcgtcacagg aaacgaggtc
    23881 cgcgcgcgaa agatttacga tacgatcaag cggcatcttg cgtgatgtga acggtcatcc
    23941 ggaagcaggt tcgcgtcacc accatgggca gttggatcct gaaattgaac cattcagtga
    24001 gttcttctgt tgacacgttc cttcctccga agtagcctcg ctgcggtcct ctctcatgca
    24061 aatacggaaa atgacggata ggaatatgaa tcggggaccg ttggttcgcc cgcattcgat
    24121 cacgacgatc tcatgataca gacagagtcg actgaaatcg ccacgcagac cagggagcgg
    24181 acgtgcaagc gggccgtggg gcaggcgatc acccttgcga tgacctccat tgcgctgcta
    24241 ttcgccttgg catcctgtgc gaagttcttc gacatgtctg gtgagcgcat taccgtgccc
    24301 ggaccggaat ggggcgtggt catcggctcg gtgctggtgc aaccggaaaa gatcgcctcg
    24361 gacgcaacag gcaagccagt ggacgtctcc ggctcttcct acgaattcga aatcgtacgg
    24421 attcagcctg gcgacccgga cggtgaaggt ccgtatgtgg aaaagtatcg gcttgatgtg
    24481 aaagccggag aagagcgaat gttcatctct cgcctgcgag cagggcaatt tctcatcaaa
    24541 agctttcgtg acgaacagat gacgggactc ggcggagacc tgggtctggt cttttccagt
    24601 atggggggcg agattcgcta catcgggcgt ctgctcgtgg aaattcctcg gcaagtctcc
    24661 aaggggaaac gctacggctt caccgtcgaa aatgcgcgcg agccgactat ggcggaggtg
    24721 tcacggcacc atgccgattt gacgaaggat gtggtggata aaccgatgca ggcacgggag
    24781 cgtcctgcgg agtaaacgac cgtctcgtct tccgggtgtg tgattgagga acagtcacag
    24841 gatcattttc tgattcaacc ccgtcagccg accacggcgt ccgcttcctc agttgcgagg
    24901 gttccctcaa gcacgtatga tagcgccacc atgtcgggac caatcacacg catcaagacg
    24961 aagacacccg tgccgtacga gctcacggct attcatcacg atacccacga tacaaagacc
    25021 ttctgctttg ccttgcctga cgatgccacg ttggacatgc tgcccgggga ccatctttac
    25081 atccacgcga cgatcgacgg gaaagcggtg aaacggccct acacgccgtc ctccacgccc
    25141 ggaatcacgg gatacttcga cttgacagtc aagcggtacg aaaccggggc agtttcgaag
    25201 tacctccatg accgggaggt tggagacacc gtgctgatga gcggaccgaa tcccggcggc
    25261 cattgggtgg atggaatggc gaagaaggcg gggtttgtgg ccggcggcac tggcattacc
    25321 ccgatgattt cgatcattcg ctggattctc gccaggagtc ttcctgtcga gttgtttctc
    25381 gtctttgcca acaaggccga ggccgacatc attttccgtg aggaatggga cgaagatgag
    25441 cgtgaacatg cgagcttcca ttgctatcat gtcttagaga agccgcctgc aggctggtcg
    25501 caaggcacgg gccgcatcac cgagaccgtt ttgcgcaagc atctgccgtc gcccggtccc
    25561 gacaccgtga tttttctctg cggccccccg ccgatggtcg acgtcctgga agcaacgctc
    25621 aaaacgatcg gctactccga acaagccatc attcttccct aaccgggtgc gaggtcaccc
    25681 ttcaccgaaa aatataaaaa gacaggcgtc gggattccaa tctcctcatt gacaggtcct
    25741 gcctgagcca gtagcgtacc ggccatgttt cgtttcactc tacacatgct gcataaggac
    25801 gaaaggtgtt gccgatgccg agaccggttc tgattttgcc cggcatcgga aattctgggc
    25861 cgcaacactg gcaaacgttg tgggagcagc gcgactcgca ctttcaacgt gtgaatcagc
    25921 gcgactggct ccggccggtg tgtgacgaat gggccggcgt gctcgacggg gcggtgaggg
    25981 cctgcctctc ggcaccggtg ctcgtggcgc atagcctggc ctgtctactt gtcgcgcact
    26041 gggcaagccg ttcaaagcgc gtgatccatg gcgccttgct tgtcgcaatt cccgatccgg
    26101 acgctcctgg atttccggcc gacgcgcgcg gatttgcgcc ggttcccctg tgtccgttcc
    26161 ctttcccgag catcgtcgtc gccagcacag acgactcgtt cggatcactc gcacatgcca
    26221 agcactgtgc tgccgcatgg aggagcgatt ttgtggagat cggcgccgct ggtcatatca
    26281 atgccgacag cgggatcggt gactggaatc aggggtatgc attgctgcag cgactgttag
    26341 cgtgatggca caggcaatca gactaatgaa acgttccaga tgtggtgagc actgacgatt
    26401 gatggctgac acagccaatg agacatccgg ctaccaggga ccggccttct tttcgtacgg
    26461 gttccggccg ttctttcttg gtgcggcctt gtttgccggt gtggcgattc ctgtatggct
    26521 cctgatgctg ggcggcgtcg gcggattgga ctcggtctat ccggctcggg attggcatgt
    26581 gcatgagatg gtcttcgggt ttttgcccgc ggtgatcacg ggctttctcc tcacagcgat
    26641 tccgaactgg acggatcgac ccccgatcag agggcgtgag ttaatcgggc tctggatatt
    26701 gtggctggcc ggacgcctgt tcattgccat gccgtggctc atgcccgttg tggccgccat
    26761 tgtcgacgcg gcatttcttg ttgcggtcgc gacactcgtc tggcgagaaa ttgctgcggc
    26821 gaagagctgg tttcacgcgc cgatgggcgc gctgatcagt ttgtatgccg gcgcgaatat
    26881 cctgttccat gtgctggcgc tgagcggcgc agagatagat cttcccgtac gcatggccct
    26941 tgccctggtg atggtcttgc tggcgctgat cggtggccgc gtcactccca cttttacggg
    27001 agaactcatg gctcaacagg gtctgtctca gcagcccgca gccttctctc gtttcgatgg
    27061 cctgtcggtc gtgcttgtcg ccatcgccgc attgatctgg accgttcagc cgcatgtcct
    27121 cgcgactggc tggatcctgg ttgcagccgg gttgatcaat ctggggcgcc tgtctcgctg
    27181 gcatggctgg gtcacgtggc gtgagccgtt ggtgctgatt ctgcagctgg gctatggctg
    27241 gctggctatg gcgttgcttg tcctcggcgg ttcgattctc gggtttggtc tcgagaccgc
    27301 ggatgcggtg cacgcgctca cgacgggcgc agtgggtgtg atgacgctcg gggtgatgac
    27361 ccgagccagc ctgggccaca cgggacgccc ccggcacgca ggtcctctca cagtctgcat
    27421 ctatacgtta gtgactcttg gggcgctgat gcgggttgtc gggcccacca ccggccttcc
    27481 gacgaatact ttcctgggat tggctgcgtt gtgctggagc agcgcgtatc tgctgttcgt
    27541 catagtctat ggaccgtttc ttcttcgtcc gagcctcgac gagtgacggc acgattcgca
    27601 gcagggacgt gcacctggca catggtgctt ttctgatcac ctcgtccgcg cgcggcgggt
    27661 tgtcgtgtgt cggcggtaac gcgagcagat atctcatctc attccttctt tgatgttgag
    27721 tgcggtgagt gttcaacggg aaatgtgtct ttacacgtga gaatgccctc acggcgcgaa
    27781 cctgccgcga gggcatcctt gtgtacccga ccgcctcagc ttcgtgagat tccccaggag
    27841 catcccgcct ccatgcgcgg cctcttctgg atgagatgag tctcctggga ggatcggata
    27901 tgtttggagt ccttctcctg cgacatgtca ttcgttcatg gcgtacctcc tgagggaacg
    27961 atcaggaaag gtggtcgacg agtaatatct tccacttcac gctaaggagt ttttcccggt
    28021 gtcagcagct cattgtccca gtctccaatc cctaaccctt atgtcgcttc cttccttcag
    28081 cctcccgctt cttccggcct accacttgaa tcggggcgca agaggtttat cgtttcagcg
    28141 tggcctgtag gcgtgacgta ggcctcaatc gtatctccga cgacgaactc ttcatctcgt
    28201 ccaccaatgc ccacttgccc tttctgcgtt ttctgatcca ccgtcatgcg cacttccttg
    28261 ccggaaccgg agttctcctt tatcgtaaaa gagttgcctt cgagctgcga aacacgcccc
    28321 ctgaccacgc ggcaatcttt gttcgcgcac aactcagaag aacgcttact cttcatcgcg
    28381 ggtgtttttc cgtcggcacc ttctgcgtcg cgtaagtctc ctggtgcttt ggcgctctgc
    28441 atgtctttca gcatcttctc caattcagga gagcgatcaa ccatctcctt gttcgtgagg
    28501 tccggagaga cctgataatg cctcgtgtcc acaaccagtt cgctggtatc cgtgtctcgt
    28561 ttgaccttca tgtgcgccca cggcacagtc tccaatgtat tggtgtcgtt caacagcacg
    28621 acggcatatt caatttgtcc cgtgttcgaa tccataatta agttttcaag cgatcctaat
    28681 agcaccccat cagagttttt gacgggactg tgaaggagcg aatctctgcc cgcgctcttc
    28741 agttcaccga ccggaactgg aagaatggca tactgttttg gagggtatcc ctggggaccc
    28801 gtcatgtcga gctgtccagg tttatcctgt ttgccaggga tcgccagttc tcctgttcca
    28861 gcactctccc cgagcgaccg cgctgttcga ggttcagtac tcctggcgcc gggcatgaat
    28921 ctgaacgtcg tcactcggcc catgtcatcc acctcggctt tgatcctgtc gccgggttta
    28981 aaggagcagg cgccaattct gaagccggaa cccatagcgg tttcatcctt cttttgcccc
    29041 tgttctttct gtcggtcgct ggcgtcggga gcctgcagtt ccgcagacat tcggtctgtc
    29101 tcaacacttt ttgacgaggt ggcttgtgtc ggagtcgtgg cggcgctgca gtcgagattg
    29161 gcgtctttgg tcaccaggag ccgcacttcg tcaccttgtt cgtcatcctt gatgaagtag
    29221 tagtcgccct caatcttctt gatctccccg gagacgaacg ccttggcacc tccgagagtg
    29281 acttgagcgt tttgatcggc gcgctgatct tttgccgggt ttcgctgttt cgcggttgct
    29341 gcctgatcgg caactgtacc tgattcaata ccaccttttt gagcggcgat ctgcggcgtg
    29401 aatcgttcct ggtctgatgg ggactccaat ccatatgcga acggaatcgt tccgaggctc
    29461 aggacgagaa caccgttaat gacgtgattg taggtcatat cgagcctcct tgttcggagc
    29521 ggatcaactc atctacttgc tgttgctcat ctgaatcgag aacgaccgtg acttcgccat
    29581 cctcgccgtt gactttcacc gtgctcaaac tgttgtcgat actcagtata tggatggcat
    29641 acaccgggct cccttcgacg atctcgagtc gtgcgtctat cgcctgtccc gggatgacca
    29701 tttctgcggc ctctactgcc tgggacatgc tgattggtat cgattgaaac cgcgtattct
    29761 cttcggtcgg tcccatgaaa ggaagactca ctccaaggcc cagcagggca atcgcagctg
    29821 tcatgtatgg gttataggcc acggatcacc tctctctgag aggatgccgc ttgtcttcgt
    29881 tcatcactcg tgagctgtct gtgcgatgtt gtttgctcca agcgctgcca catcagttgg
    29941 cgactgcagc ttgaaaactg ccctgcgtgg acatgaacag gacaagcgcc cagaggacgg
    30001 tcgccacgat gccggctgca accatggaga tcaacaagat caacacgctc aagcatatgt
    30061 ttgaggttca tgatgatctc cgctgcctcc ttgctcttcg ctcttccgac aatgaactgc
    30121 cactcatact cgtcccccta aaggtatgag gcaaatgacg gaccatcgat acccgagatg
    30181 tccagctctc tattttttca ggaagatacg tgtgcctggt ttgcgcctgg atgtgacgct
    30241 ctgaaacagc ttgtccgaga gcggaactgt agtctttcga actggctgtt tttccgtcac
    30301 atctggatgg cgaacgtgtt gtctgccttt ggacactgtg tccgtatgcc tgatgaaggg
    30361 atgggaatca tgtgactcgt tctcacgcgg cggtttggtg ccaccatcga gcatagggtt
    30421 gagaggaaat ttggaagagc cggacatgcc agctgtccgg actggcgatc ggccaattcg
    30481 tgttccggtt gaagagatgc ttgtatgaac gcggcggagt tgctgtttcg ttgcctcgag
    30541 catgagggtg ttcaactggt cttcggtttg cccggagagg aaacccctgg agttgatgaa
    30601 tgcgctgccg gattcccgca cgcgatttat cgtgacgcgg cacgattagg gaccagcgtt
    30661 catggccgat gtctatgaaa gacttcacga aaaggcggga gtctgtctgt ccacgctggg
    30721 acccggtgct gtgaatgcct acctggatcg tgccccgctg gtcgccatta ccgggcaggc
    30781 ctcttcggcg attcaggaat tacttacgaa tcgtgagcgc aactgcaaga gagatggttg
    30841 cgcagaggct caagtagaag gcgggcgcca ggctgttgcc gctccattca ataagtactg
    30901 tggcgatcaa cggagcagta ccgccgaaca aggcggctcc gatgttgtag ccgatcgaga
    30961 ggccggtata gcgtagcgat gtgggaaaca attcggcgac tgcggcaaag aacggcccca
    31021 tgtagcaggc gaccagaatc gtcaacacaa tttgtgccgt gatcatgcgt ggcaggtcct
    31081 ggctcgtgag ccagagaaag caggggtatg acaagagcgc gattcccgcg gcgcccgcga
    31141 tcagcagagg cggttgtccg attcgatctg accaggctcc catcacggga atcaacaggg
    31201 ccaggatcgt catgcaactc gtattgagaa cgagcacggt cgcgagtgga gtagttgtca
    31261 cagtcaccag gtaggtcgtc agatagacgt acaacagata gaacgcgacg ccatgcaacc
    31321 acacgaggcc gatgactttt gccacagcac gtcgctgcat cgtgaagacg tcgcggacgg
    31381 gggatgctgt cacgtttcct gcctgacgaa ttgcttcgaa ggccggcgat tccggaactt
    31441 tggttcggag gtaccacccc accacggcga tgaagccgcc catcacgaag ggaatccgcc
    31501 agccccactg acggagcgcc tcctccgcga gactcgacgc cgtcagcgct cccacgcctg
    31561 atcctaacaa agcgccgatc tgggcgctga agccggccca gctcccgaca tatccgcgtt
    31621 gcgacgctgc cgcatgttcg acgagaaagg tggcggaccc ggtgaattct ccgcccaccg
    31681 acagcccctg gagaaatcga cagagcgtca gagcgatcgg cgcgaccaca ccgatggtcg
    31741 cgtaagtcgg caggagtccc accaggcaag tcgggatggc catgaacagg accgaccagg
    31801 ccaacgcgtt gcgtcgtccc agcgtgtcgc cccaatagcc gaacagtaat gccccgagcg
    31861 gtcgtgccag aaatccgacg gcgaagacgc cgaacgcgga gatcaacgag atggaaggag
    31921 agtcggacgg aaaaaacagg gcggcgaaga cgggcgcaaa gtatccatag agggcgaagt
    31981 cataccattc gagcacattg ccgatcgctc cggcgaggag cgttctcttc atcgcggacg
    32041 ccggggacag gctcatgcgg tgttcgaggc tccggtgtga gaaccgtacg gcttgtccta
    32101 gcctgcgaca aagttgaacg cagatgcaac tgtttactcg gtgtgaatga cacgaacggg
    32161 tgatcgttcc tgattcgggt taaggagcgg gaaccttgtt cctctccttc aacaagcgcg
    32221 gcgttattgt tgatcccacc attggtcggg tgttctcatt gacagggttt gccagagcca
    32281 gtagcctacg ctctgttttc tttcacccgc ggaggcagct atgaccactg ttgcagagat
    32341 cgcgccagac ctgtaccgga tttcgacttt cgtgccggat ttcaatctgc agttcaatca
    32401 gtttctggtg cgtgatgaag agccgctact gttccataca ggcccacgcg cgatgtttcc
    32461 ggctattcgt gatgccgtgg cgacggtgct cgatccggca acagtcaagt ggatcgggtt
    32521 cagccatctc gaagcggatg aatgtggcag cttacgggag tggcagcagg tggcgccgaa
    32581 atcgatggcc ttgtgcggtc tgatcggcaa aatggtgagc atcgacgatt gcatggccgc
    32641 gaggccagcg gtgggcatga aggatggaga ggtgatgagc accggcaagt atcgattccg
    32701 gtttctgcat acgccccatg tgccgcacgg gtgggatgcg ggtctgctgt tcgaagagac
    32761 gcagcggaca ctcctgtgct cggatctgtt tcatcaggcc ggtgaggtcg agccgtggac
    32821 acactcggat ctgttgggcc ggtgccgcca ggtgcttgtc gagtatcagc aggggccact
    32881 ggctaactat gtgccgtatt caacgttgac ggatgcgaca ctccaccgac tggccgggct
    32941 gcagccgaag acgttggccg cgatgcacgg gtcggccttt gtcggcgatg gcgctcgcgc
    33001 gctccgcgat ctggctgtca tgttcaaaga ggtactcggt tcgagtacat agaagccgtt
    33061 cgctcggctc cacgacacta tgtgaatcgg cgccctcctc gccgcaatga ctccctccgc
    33121 gatttaatca acgggtgggg caggtaccat cagtctcagt gagatccgat ggacttggcg
    33181 caagtgctca ttgcgataat cggtctcgga gccgccctcg tgagtttctg tctcatgcgg
    33241 tttccgcaat accgccgtca tctcttcagg cagttatggg gcattttcat tcggtgcttc
    33301 tctgctcggc gcagccgcca ttcttgcctt gtgtatatca atgatcctgg cgcaaggtgc
    33361 ctggcagtct cactagacca gagcaagatg gaacggtgtc gacccgctgc ccctcatgca
    33421 cacgggttgt agtcgtttat cgaccggctt gacggaggcc ctgagagaga gatggctgcc
    33481 actcccaggg cccgtcgtgt atgcggggtt agcccggagg cagcgcgaag tacgtggcaa
    33541 tggccaggat cggcgctccc acgtacaagg cggtcttgcc gaaggtatcg tacaagccgt
    33601 aacacaagac tacccacatg agggtgtagt acatcgtggc aatgccgcga taggttcctc
    33661 cggctccttc cgccaaggcg aacgtcggcc accaggcggt gaatccgacc agtaacgcga
    33721 gcagatatct catctcattc ctccattgag gttgaatgca ggtcgagaga tcaacggggg
    33781 aggtgtgtcc gaatgcgacg atgccctcac ggcgcaaacc tgccgcgagg gcatcgttgt
    33841 gtacccgacc gactcagcct catgggattg cccaggacta tcccggttcc atgcgcggcc
    33901 tcgttctgta ggaggaggac aatcctgccg tttctttctt cgtctgtcaa ctggggggag
    33961 gcgaaagcaa aaaaagagat tgggtgctct ggcgcagcgc ctcctccgcg aggggggtag
    34021 cttctgccag gacgtagcca tcggcttgac agttccccac ggaccgccta gcctgaaatg
    34081 gaatcttgct ttaaagtgga tctccaaatg ggacccatga gaccgaagcc atcgaaaaag
    34141 atcagcaagc ccgccgctga ccgtgccaag gcaaccagtc aaatacctct gccggaagaa
    34201 ctccatgaac agatcgccaa gcgagcctac gagctgtatg agcgacgcat ttgcaaaggg
    34261 gctttggatg actggctcca ggcggagcag gaaatattga gagaaacaaa aacacggaag
    34321 gctcacccgc cgcatcgtgg gggatatgcg agcgaggagc aagaatgaca aatgagtgtc
    34381 tgggcagctt gcaatgaatg caggaagcac gagattacga gtcagaagcg gtagcggtgc
    34441 caggtaatcg tgcaggattg ccatcaaagg acgtgcctca tggagccagg ttgtgagcgc
    34501 acgaccattg ctcgaattgc tgcagctctc gggtgtctga gcggaggtat cggcctcctg
    34561 accgcagtca cgaatcagcc gttcgggttg acgccgcacg gatgggtacc ggcgcgattc
    34621 tgctcttgct gatggccctg ttcgtgctgg tcgagggaat ggattcgttc gagaaatctc
    34681 aactcggtgc cgttccgaaa cattaaccga acgggctgat cggattattc cgggtttctg
    34741 atttcacttt agtaagatac agtcggtgga attagttcgc tttctttgcg tcggactgct
    34801 cctgatccgg agatcacagg tatcttttat tgacaggctg cgcgcaagtg cgtagcctgc
    34861 tcgcggtttc ccagctcatc ttctgaggcc atcgcaccac tttcacgagg aggcgactat
    34921 gccgacattc ctgcgagcct atgccccgca cacctatgcg ttgatgcgta tcgtcaccgg
    34981 ctttttgttt ctctggcacg ggacgcagaa attattgagt gtgccggtgc ctcctccgcc
    35041 tcaagtgcca tcgtttgtca tcgccattgc cggcccgatc gagttgatcg gcgggtcact
    35101 agttatgatc gggttatttg ccggctgggc ggcgttcctc tgcagcgggc tcatggcgtt
    35161 tgcctattgg atggcgcatg gcacacaggc gctcttaccg cttcaaaata tgggcgagct
    35221 agccgctctc tattcttttg tcttcttgta tatttccgct cacggctcgg gtatctggag
    35281 cgtggaggcg gctcgcaatg cgagctagcc tgactcccga catcatcttg acagaggtac
    35341 acatcgtggc tagcctggga tgctattgtg gttcgaatgg ttggtaggta gagggggtgt
    35401 ggaggatctt cctctggagg gcccgatcat gcgccacaag cgacgcatcc atgatgctat
    35461 tgcgggactc ccggtgacgg cgggagtggt gttgggccac tatgtgagcg cacactggct
    35521 cattctgcct gccgtgatcg gtgtgactct gattcagagc ggcctcacag ggttttgccc
    35581 cgtatacttt gcactcgatc ggcttcttcc ttccgagagg gcggccgatg catccagctg
    35641 ctgcgccaag tagacaggat gggctgcgtc tgttcaaggt gtgtttggca taggtttcga
    35701 cgaggcgtca cgtttatcac ggaaggcgtt cctttcgtgc agccggcgaa ccaattttcg
    35761 ttccatgttt gtgcatctcg cagtctgtat ctagccgatg gcgattcgta tcactcttgc
    35821 acagtccagt gacgccgacg tgattgcctc gatggtgggg aaactgctcc acgagatcat
    35881 ggccgcggta aagcacaaag cgtttggatt tcatcaaggg gaaacggcga cgcgcgtccg
    35941 cacatggatc aaggatggtg cgtatacggt attgcttgct cgccaggaga gggttgcgga
    36001 accggttggc ttcctagcac tgtacgagag ttatgcgctc tacgccgaag gcaagttcgg
    36061 cacgatccct gaattctatg tgtctccggc gcaccgctcg aagggagtcg gctcggcgtt
    36121 attgttggaa gccaaacgga caggggagac aaggggatgg gggcgactcg aagtgacgac
    36181 accgccgctt cctgaatttg atcgaacgct ggcgttctat caacgtcacg ggttcagtgt
    36241 ctcgggcgga tgcaagttga aagtcgcgtt gctgtgaagg cggttgtgca acttgaccgg
    36301 accggctgcg gcctcgccag cgtggctgtt gttgccgggg tgcgctatcg gcaagtccag
    36361 cgtgtggcaa accgcttggg gatcttcgcc gaggatgaac gattgtggtc tgagaccggc
    36421 tatgtgcgcc gcttgctgcg atactacggt gttcgcgcgg cgcagactga ggttcggttt
    36481 cggtcgtggg agatgttgcc ggatctggcg ctcatggcga ttaaatggca tcgggttcat
    36541 gggcgcgcgt tctggcattg ggtcgttttc tggcgtggac ctggggggcc ggtggtactc
    36601 gatcccaagc gcgggcttcg cacgaatgct cgcacggact ttggtcgcat ccgcccgaaa
    36661 tggtttcttg ccgttacaac atcaaagtga cgagcctgtg tcgacaggga gttgccctgt
    36721 tgacagcgat ccactgtatt tcgtacggta aaaaaggggg gaggatcaat catgccaatt
    36781 cttcttgctc tctttctcgt cttcgcactg gttcctcaag cagcggcatt ggagggatct
    36841 caaccaactg catctccgat gatcaagggt gatgtgctgt actgggaagg tgaggaagtc
    36901 atcgtgaagg agatgtccgg gagggaagtc cgcctgcgtg taaccccgga aacaaaactc
    36961 gagggtgtga ttagtcgact caaaaccggc gacaagatca ccgcgcaggt caactccgat
    37021 gggcgcgctc tctccattac tttgcagatt ccagacagcg ggcctagccc taccactccc
    37081 ggcttgcgtt agcgcgcaag tcctgtggcg agaatgcttc gctattgcgt gatgtatccg
    37141 gaaactatcc gtcgtccagt ccgactcgga tcgcccattg tcccagccag gctactctcg
    37201 ttcttgaggc gctgcgatca tattcggaat acagcgttct ttcctcgcgg ccaatgcgta
    37261 ggcccaagag aatgattagt cgctccaaat cctttccaaa ctcgatggaa taggggacgt
    37321 cgttcttgaa ttgctcggcc acaaatgccg ggtcgtgatt cttgccgaaa aactccatcg
    37381 cctccgacgc gatcgcttcc acatccttgg cgaaaatctc gagagtggat ttcaatggcg
    37441 gatcagtctc cgcgagtgct ctgagcgtgg ggtggagctc ccgattgtcc tattcgagat
    37501 ggctgagcag agcgccagta gcgtcattca agatcgcaat gcgttcggtg gcggggcgag
    37561 tgaaatccac ggttttcttg agggtctgct tccgtgtggc atgatcggat tttcgttgtt
    37621 caataagttg cgacattgag caatcttcct tctcaggaca tcaggtctcg atgtgtgcat
    37681 gcacggttgc gcgactccga ggtgagatct gcgtggagcg tgaaattatc ggatgagtgt
    37741 agcaggaggt tgccagtggt aaagtatcct aattagtgtg aatgccttta atattctgac
    37801 cgtgacgatg cgggtttttt gaaagggcag ttgtcgcaat aatccgtgcg agtctttgtg
    37861 ttggaattca gagaggaaaa tacgcgaata gtagtatttg tctgtcaggg tattgaagag
    37921 ctttaagcca gatgcctgca ggttttcgca ggcgagcatc gatcagtggg cgatcgagcc
    37981 aaacgactga ccggttcagt tcaagtgccc gcgatgctcg accggaggca ggcctttcga
    38041 acgcgtggtg gagccgtttc gaggggagtt acttcggcgg gaagtgactg aacatttcgt
    38101 ggacgaggcc cctgatacgc tcgtcccgtt cctcggaggt cagcccagga ttaatttctc
    38161 cggacgcggt gccgcgccag accagtttct ttgatgaggc gtcaataacg tcaatcaaga
    38221 gcgcgcctgt catgtataac ggcttgtcgc tgaccggccc ctgctgtttc ttacccatcg
    38281 tgcgagtatc agcggaatag gtgaaagggt gaccggtcat gccttcggag aaatattggg
    38341 tggacgaatc gaggctcata ctattgaccc cgatatggta ggcgacataa aaatccggct
    38401 gcttgttttg cggtttggta tagcccttta aatgcagctg cgctccgacg gccgtgcgaa
    38461 tgcgaatgtc gacgtccgga ctgtcagcgc gtcgatcgcc ggtcttctcc tgtttaggta
    38521 ccgcccactc gtaggtatga tagtcgctga aattcgcgct gggatcgtag tcatacccga
    38581 ccttcgctgt ttcacaggcg accattgatg aagtgagcag gaacaaacag agtaattgta
    38641 ttcgtgtcat aagtggcctc ctggcgcggg gatagtagca gaaccggccg cccaaaagaa
    38701 ggcacctgcc gctggcatac cgacttgttc tgatggacgg atgccgagcc tctgctctcg
    38761 agaggacatc acggggtcgt tgtgcaggag aacaccgatc cggggaaggc cgtgtgtttg
    38821 atgcaaatta cttaggcggg aaatgagcga acatggcgtg gacgacggtg ttgatgcgct
    38881 tgtcacgttc ctcgggagtc atggccggat caacttctgc cagcgccgac ccgcgccaaa
    38941 ccagttgctt cgatgcggcg tccacaatat cgattagcag ggtgccttcc ttatatggct
    39001 gaatatcgct gatcgtggtg tacgtgccgt gcgcccgatc accgatataa tcttgagtgg
    39061 aagatccctt catcatgtcc ttgaccttga tatggtaggc cacatagaaa tcaggctgct
    39121 tgttcacgga catcgtgtag ccttttgaac gaagctgcgt tccgatggcc gttcgaatgc
    39181 gaccgtcaac cagcgaattg tcaaggcgct ggtctccggt aacctcttgt ttgtctgcca
    39241 cccaagcata agtgtgataa acggtgaaat tcgcactgga atcgtaatcg tacccgacct
    39301 ttggggacgc gcaggctgcg actaatggcg caagcaggaa cagacagaga gattgtagcc
    39361 gtgtcatgcc cggtctcctt ccgggggata gtagcagaac cgatgggcca agagaagctc
    39421 gcgcatcaga cgttggcact ctcttgcgag tcagcctggg acggaacagc atgagcgggc
    39481 agtcctgtca ggaaaaccgt cgctgcgtcc cgcgcagggg agcggtcgta ggccgatcct
    39541 tcgaacgagt tttcggcgag tcatccacca cagggtttcc gctgtgttcc gtaaattcat
    39601 gagtagcgct ccacgctaca cgctgtcgac cgccatatgg tatgctgcct acccatgatg
    39661 actaccgata cctcgttgca gaatgaactt gccgctgcca cggggaaacg tcgtacgttt
    39721 gccatcatca gtcaccctga tgcggggaag accacgttga cggaaaaact gttgctctac
    39781 tccggcctca tccgcacggc ggggatggtg cggggccgca agggtggaaa aacggcggcg
    39841 tccgattgga tgggcatgga gcaagagcgc ggcatttcga ttacggcctc ggccatgcag
    39901 tttccctata aggacgcggt cattaacctc ctcgatacgc cgggccacca ggatttttcg
    39961 gaagatacct atcgcacact caccgccgca gacagcgcga ttatggtgat cgatgcggcg
    40021 aagggcgtgg aagcgcagac ccgcaagctg ttcgcggtct gccgcatgcg gcgtattccg
    40081 gtcctgacgc tgatcaataa gatggacttg ccgggccgtc caccgttgga tctcatgacg
    40141 gaggtggagc aggcgctcaa cattcacgcc agcgcaatca attggccgat cggggcggga
    40201 agcgacttcg tcgggatcgt aactcgtgcg gacagtcgcg tgcagctctt cagcaggacg
    40261 gcgcatggcg gatccacaaa agtggatgtc gccaatctgt tgctggcgga tcttgcaagc
    40321 agcggcaagg tctccgacga ggttatcgca gaagtccgac atgatttaga tctgcttgac
    40381 attgccggca atccgtttgc gcgtgagccg tttcttgcgg gtgaagtgac gccggtcttt
    40441 tttgcctccg ctcttaccaa tttcgggatc gagagtttct tggacgcttt cgtagaactg
    40501 gcgcccagtc cgagcgcgcg actggccgac cgcgacgacg gcaccgaatt gacagtcgat
    40561 ccgattgaga tgccgttcag cgcctatgtc tttaagctcc aggccaacat gaatcccaag
    40621 caccgtgaca gcacagcgtt tttgcgtatc tgttccgggc ggtttgaacg cgacatgatg
    40681 gtaaagcatc accgccttgg ccgtgagatc cgactgtccc gtccgcatag cctcgtggcg
    40741 caggaacgca gtacggttga ggaggccttc cccggcgaca tcatcggtat catcaatcct
    40801 ggcttgttcg ccattgggga cacgatttcc gtaaccgggg ggtttaactt taaacccttg
    40861 ccgcagtttc agcctgagat atttgcccgc atccgcccga ccgatgtcag caagcggaaa
    40921 gcgttcgata aggggatgga gcaaatggcg caggagggca cggtgcagat catgaaaagc
    40981 ctcaatgacc tggaatcgct ggtagcagcg gtagggcggc tccagttcga tgtgctgcag
    41041 tatcgtctgc gcgatgaata tcgtgtcgaa acggtgctcg atgctttgcc ctacacctgc
    41101 agcgcctggc tggaaggtga tccaggcaca ttcaagtcac cgtctgtgtc gatgatggtg
    41161 aaagaccaac gcgggcgcgt cgtcgtgttg ttcggagacc aactcatgaa gaccatcgcg
    41221 cgcgaccgga atctgaacca tgtcttgaag gacatgggat aagctttttg ttccatggcc
    41281 aaggatctcc aaatgaagta gaccatcctg cggctgaact cgcttggaaa ttgtgctgtg
    41341 tctgagagaa tcctgcccag ggtcccccct ctcctgtgtc tcctttccaa taaacaaacc
    41401 gtatcagaat cgattctgct gtgatcctaa ttgattcagt tttcgaggtc ttgcgcatca
    41461 aatccccata gaaatcccta tttatcgcct ttcgagcttg gcgcattctt tgctatgcat
    41521 gttattcggt gaagcgagat tgagtatccc gatccacgat gaccggggag aatgatgggc
    41581 attcgaatcc ttgttgccga tgacgatcct gatatcgtcc tgagtgtatc ggaacggctc
    41641 cgctggatgg ggtatgaggt aattactgcg agcgacgggt gtgccgcctt gagcgcggtg
    41701 gagtcccaca gtcttgatct tgtcttgatc gatgtatcaa tgccccgatt gagtggaatt
    41761 gaggtgctta agtggatcag gccacgatgg ccgaatctgc cagtcgttat tttgaccgcc
    41821 tatggcacga tccggcttgc ggtagaagcg atgaaggaag gcgcagtcga tttcatcgct
    41881 aaaccgtttg aacatggtca aattgacacg gtggtcacca cggttctcgg gcggagtgag
    41941 caaagggtgg aaatgacaca gctgatgggg gagattactc acgacgttaa gaatctcttg
    42001 atgcctctgg tcacaggcac agaccttctt gcggaggaga tcgacgactt attcaagaat
    42061 ctgccggaga ccgaatccgt ccaagccgaa aagagccatc atgtgtgtga agaagtcatc
    42121 cacttgctac gcaacacatc ccggcgcatt caggatcgga tgaaaggaat tgcggactac
    42181 gcggcggtgg cccgcgcctc ccaaaaattc gagccctgcc ggatcgcgaa gatcgctgct
    42241 agtgtaatga agtcgcttcg cgtgctggcg gagcagaaac gcatcacggt caggttcgag
    42301 ggactcgaca agttgcctga catcgtcgga gacgaaccgc gactctattc actgctctac
    42361 aacctcgtga acaatgccat tcccgaagtc cctcctctcg gttcaatcac gatttgcgga
    42421 cgtcatcgtg ctgacgatga tttcatcgag cttgccgttc aggatacggg aaatggcatg
    42481 ccgcctgaaa tacgagacag tctcttcacg aatcgccttg tgagcatgaa agccggtggt
    42541 acaggattgg gcacgaggat cgtgaaggat gttgtcgata tgcacggtgg acagattgcg
    42601 gttcacagtc aggaggggaa tggcaccacg tttgtcattc gcctgccgat tcacccgttg
    42661 gccctcgtgc aaaaagcagc aaaaggcctt tcggagtcac agattgccca tcccgattag
    42721 gccctgatcg caccgtcaca taggtccaac catagggtac ggtcctttcc tcatcgccaa
    42781 atcgagcgtg cgggatccag atggggatgc ctatcgcggc gcggtggatt tcatcaaact
    42841 atcggcttgc gggggcagga atccttggta gccgcgatgc cgttcagcca gctccaggac
    42901 tgaaaacggc tgaccgtcga ccatttctgg tgctgaaaaa atctgccgga gttcccctcc
    42961 aggcggtccg acaatctttc cggcaaagac gactcctttc tttctcagtc gttcgatggc
    43021 cttctcgatg tcttccacgc gtacagccac atggtggaac acctggtctc caaacttctt
    43081 cacccatccg ggaatgatgc tcgtcttgcc gcggtcgtcc ggatagcctt gatccacaaa
    43141 gagggcgggg tagccggctt ttcgatagac ctttgcgtac cagtcgtcgt agtgaagtgt
    43201 ttcatcgtag gtatagccga aggcgacgaa ctgctcggcg cgtcgatcga tgtccagcgt
    43261 gcgcaacgtg acgtgatcta cgaccggtac gaatccgaca cccgtgtcgt ccagcatcgt
    43321 tttcaggatt ttggccgcac tgttatgcgc cacatagtcc gagatcatcc gctccaggat
    43381 ttcatcgagt tcgcgttggt gatccataat agaacctcgc tgtgtcgtgc ttcgaaccgt
    43441 taggatccga actgaatgcc ttgcgcgagg gggagttccg tgccccagtt gaccgtgttg
    43501 gtttgccgcc gcatatatgc cttccaggag tcggaccccg cttcccgacc accacccgtt
    43561 tctttttctc caccgaacgc cccgccgatt tccgcgccgg acgtgccgat gttgagattc
    43621 gcgatcccgc agtcgcttcc cgctactgaa agaaaccgtt cgccgtgccg gagattgttg
    43681 gtaaacatcg cggaggaaag gccttggggc acgtcgttct gcgtccgaat ggcctcatcg
    43741 atcgtgtgaa aggccatcac atacaagatg ggggcgaacg tttcccgttg cacgatcggc
    43801 cagtggttcc gcgcacggac aatcgtcggt tccacaaaat tgccggggcg attgaacacg
    43861 cgcccgccgt agagaatttc ccctccctcc ttcttgacct cgtcgatcgc ctggcggtag
    43921 gcctggactg cggcttggtc gatgagggga cccagcagga cattcggctc gagcggatta
    43981 ccgatcggaa tgtggctata ggccttcaca agcttgtcgg tcaattcgtc ggcgcgcgat
    44041 tcatggatca ggaggcgcct ggtcgtggtg cagcgctggc cggccgtccc gacggcgccg
    44101 aacaggatgg cacgcacggc catatccaga tctgcggtct cgtcgacgat gacggcgttg
    44161 ttgccgctca gttccaggag cgtccgtccc aggcgctgag caacggcagt ggccacgcga
    44221 cggcccactt ccacagagcc ggtaaaggaa ataagcggca gccggacatc cttcaccatg
    44281 acctcggcca gcgccgcttg ttcggtaatg aagagggaaa acaccccggt atagccctgc
    44341 cgctccatca cctggttaca gatgtgctga acggcgattg cgcagagcgg tgctttcggc
    44401 gatggcttcc aaatgactgt atcgccggcg atggcagcta aaaatgcatt ccaggcccat
    44461 actgccaccg gaaaattaaa tgccgtaatc acgccgaccg gtcccaatgg atgccattgc
    44521 tcactcatcc ggtgcgctgg gcgctcggat tgcatcgtgg cgccgtacat catgcgtgat
    44581 tgcccgaccg caaagtcggc catgtcgatc atctcttgga cttcgccgtc tccctctgcc
    44641 ttgatcttgc cgacttcgag cgagacgagc gtgccgagtt gatctttctt ctctctcagc
    44701 gcctggccga tcaaccggac gagttctcct cgtataggag ccgggaccga tcgccaggac
    44761 ttgaatacct cacgtgattg ttttgcgata cagtcataat catttgccga acatgcatgt
    44821 acgccggcaa tgacctcgcc acttgccgga ttaatagact ggagcagaga accgggtgtc
    44881 tggtcggacc accaaccgct accggtgctg ccgccagagt tgacggcttg gatacccaga
    44941 tccttgagcg cagcgtgcac ggtgctcatc gaaaacaggc tccgaagtcg ttggccagaa
    45001 agtctttcag cggtactgat tcctgggtga tgaatccatg gtaccgcgac gggtttttca
    45061 agacgagatc catgacacag cagacactgg aggcggtcgt gacctgaatg gctgaccaga
    45121 gcttgccctt gatgcattgg gggtagatct tcttgacata gttctcttcg aagaagccgc
    45181 cgtcttttgt gccggttacg gaggcataaa tcagcacgac gtcttgcagt gtttggggaa
    45241 tggcatgttc caggacgcgc ttcagtgtct cgcggtcttc attcagtttc agatccttca
    45301 tcaaaagatg aatcttttca caatggccgg gatagcggag cgtcttgtag ttcatcgtct
    45361 ggaccttgcc cgcatagcta tccgccaagg ttccgaggcc cccggacgtg ttgaaggctt
    45421 cgtagagcag accgtccaat tctattgttt catagccttc caagggttgg agcgggacct
    45481 tctcgccttc ctcaatccca tagcagaggt taccatattc gttaatgagc ccatcggtcg
    45541 accaggtcag agaatacttc agtgcattgc tcggatgaac cggtaaggcg ccgacccgca
    45601 tcttcacggt atccaaggtt tcgaaatggg tcatgagatt attcgtcacg atgctgataa
    45661 agcctggagc gaggccacat tgcggcataa aggctctggt cgcgctcgcg ctcaagatcc
    45721 tgatctgatt cgtaacttcg acatcttcgg tgaggtcgaa gtaatggacg cgatgcgtga
    45781 gcgccaaccc cgcgacggtg gggttgcagt aatagggaag gctggagatg atggcatcga
    45841 caggatgggc ggagagatag gtgctgatgg catccggatg ccggacatcg aggatgctgg
    45901 gtgtgacatg ttcgaggccg agatcctcaa cgaggcgttt cggcgcatcg agattcacgt
    45961 cgccgacgtg gacctcgtag tttccacgct gggcaagcag gcacgtgacc agagaaccga
    46021 tctttcccgc gccgaggaca agagcccgat gcatacacga cctcgcgtat gactagtata
    46081 ctcttgtctg tagttttggg cacattcgga tgtgaaatgc gacaaagggg agggtgctgt
    46141 caatgagctg gcgtcgatga acaatccaga acaaactgcg ggcgggagtt ggaacctgac
    46201 gggctttcat ggcattggaa agggggaata tctgattgca atcgtcgcag taatgttttc
    46261 atcttcatcg aagagggaat ttcacagagc cagacatgca tgccggattc caattcgtcg
    46321 aaatggcgtt ggacgcgaaa gctgggctgc tccataaaat aggcggagag aaatccgttt
    46381 atggcctgca ccacccaggc gtgctcctta acgtaacggt agctcaaccg caactccacc
    46441 acatgattgt cttgtccggg atccatggcc tgccgaccct ccggctctgt tgtaatgccg
    46501 cactctatca attcctccgt tgagactgct actcggatct gtgtatggta tggaaattca
    46561 tccggaggcc atctcgtgac attgaccgcc tataatcagg taccggttcc ctcctttatg
    46621 tacggtacgg cgtggaagaa agaggccacg gcgtcgttgg tgctgcaggc cgtcgaagcc
    46681 ggttttacgg cgatcgatac ggccaatcag ctcatccatt acgatgaagc gctggtgggg
    46741 gaggcgctgc tgcaactcgc ccgacagggc atcacgcgtg acaagctgtt cgtccaaacc
    46801 aagttcaccc cggtcgacgg acaggaccat cgcaccccgt acgacgtcaa ggcgagcatc
    46861 accacacaag tgcaacaatc cttcgaaagc tcgctggtcc accttcatac agactctctg
    46921 gattcgtacg tcttgcacgg tccctactcc cggcgcggct taggagctga agactgggag
    46981 gtgtgggcgg cactcgaggc gctgtacgag accggtagaa cgaaaatgat cggaatcagc
    47041 aacatcacgg cagaacagct ctcactgctt tgtaaccatg cgaagcacaa accgatggtg
    47101 gtgcagaatc gttgctacgc ggcgttcgga tgggataagg ctgtgcggga gatttgccgg
    47161 acccatggca tgatctacca aggtttctca ctgctgacgg ccaatcgcga ggtttttacc
    47221 gaaccagatg ttcatgcgat ggccgccagg tacggggccg gcctggcgca gattgtgttc
    47281 cggtttgcca tgcaggtcgg catgctgcct ttgaccggta cgacgaacca gcaacacatg
    47341 aaagaagatt tgcgatcgga ccagtttact cttactccgg aagaggtgcg ccgaatcgaa
    47401 acaatcggaa tgtaatggcg ggcataaaag taagagtaaa gcgcatctac gagcctgttt
    47461 caaaagccga cggcttccgt gtgctggtcg atcgggtctg gccgcgaggg atttcaaaag
    47521 ccggggcgcg catcgacctc tggctccggg atatcggacc atcgaccgct ttgcgtcgtt
    47581 ggttcaacca cgatccggtg agatggaagc agttctgtac ccgctatcgg gctgagctga
    47641 ggggcaagcc tcagctgctt gccttgttga agcagcatgc gcgctctgga gctgttacgc
    47701 tgctgtattc tgcgcgaaat gaagagtgca atcaggcggt tgcactctgt gccatgttga
    47761 agcagccaag gacacgacac gttcgggttc gccagcggcg ctccgtatga atccctggcc
    47821 aaagatccgc tgtggctggg ctggctccaa gccgcacctc attctctatc atgatcgaga
    47881 atgggggatg ccggttcacg atgatcagcg gctgttcgag atgcttttat tggaaggggc
    47941 tcaagccggg ttgagctggg agacgatatt gcgaaggcga gagggctatc ggcaggcctt
    48001 tgccggattc gatcctgtga aggtggcgcg attcgatcgt caaaagaagg gggcgttact
    48061 caagaacggc gggatcatca ggaaccgctt gaagatcgaa gctgcggtca ctaacgcgca
    48121 agcctttctc gcagtgcagc aggagttcgg aacctttgat cgctatcttt ggcagtttgt
    48181 cggcggatca ccgcgcatca atcggtggcg gaagatgtcg gatctgccgg caaccagccc
    48241 ggaaagcgat gccttgtcca aggatctcaa gaagcggggc tttcgctttg tcgggagtac
    48301 gatcgtgtat gccttcatgc aggccgtcgg catggtgaac gaccacaccg ttgattgttt
    48361 tttgtctaaa aaacactctc gcacatagaa cgggggggca aatagaggga gggttcccta
    48421 tgcggcgtcg gttgacgtgt tttccagggt attcagatat tgtttcaggc gctgttggga
    48481 atccggcgca atacggatga attccacgcc gaaactgcct gtcttgaccc aacgaaccgg
    48541 agccaattcg atctcaatgg ctccggcttg agcagggaac gtaattgtca cggtcaaata
    48601 ctctccgcgg gcgatcgcgg tatcgctgag aattgcagcc ccgccaagcg agaggttatc
    48661 aaggcgacct tcgccgttga tcgtctcact cgaaaaggtc acgagcgagc tgtcggtgaa
    48721 gcgttggtat ttccgttgtt ccatcgggcg caggaagtgc tctgtctgtg ccggccatgc
    48781 gcgccgctta aagtcggtcg cgcttccata cagcgatgat agcaagccgc cggtcatcgt
    48841 caatgagtat gtggaatttg gacaggtaag gttgtggaac gtcgaccagc aatgaaggtt
    48901 gcagtcatcg ggggaggtcc ggccgggctc atggctgctg aggcggcaag ggcagcggga
    48961 gcgatggtcg aggtctatga tgcgatgccc tcgatcgggc gcaagtttct tcttgcagga
    49021 aaaggcggtc tgaatctgac gcattcggaa ccgctggaac cgttccttgc gcggtacggt
    49081 ccccgccgcg ggcagattga gccgctgctt cgcagctttg gacaggaggc gttacgggcc
    49141 tgggtgcatg ggctcggaat cccaacattc atcggtacat ccggtcgggt attcccgaaa
    49201 gacttgaaag cagcgccgct gctgcgcgcg tggctgcgcc gattgcgagc atccggagtg
    49261 caattccatg tgcgccaccg ctggagggcg tgggatgacc ggggcgccct gtgttttacg
    49321 acgagagaag gaataaaacg cgtggaggcc gatgcgacga tcttggcatt ggggggcggc
    49381 agctggccca aacttggttc cgacgccgcc tgggtcccgt tcctcaaaca gcggcgggtc
    49441 gacattgccc cgttgcggcc ggcgaattgc gggtttgata tcaagtggag tgaccatctc
    49501 cgcacgaagt tcgccggcta tccggtgaaa tcggtggcgg tggtcgtaaa aaacgagcag
    49561 ggcggtgaat cgtggcaccc cggtgagttc gtcatcacag agaccggcgt ggaagggggc
    49621 gtcatctacc tcgcgtcggc gattctccgt gatgaaatcg agcgcaacgg gactgccacc
    49681 ctctggctcg atctggcgcc ggaccgggat cttccacgat tgacccgcga tctctccaag
    49741 cctcgtggca agcggacagt ggcgtctcat ctacggcgct atgcacggat cgaaggtgtc
    49801 aaagcggcat tgctccgcga agtgatcccg aaacaggatt ttaccgatcc tgctcgatta
    49861 gccgctgcca tcaagaggct gccacttacg cttgttgcaa cgcgtcccct cgacgaggca
    49921 atcagcacag cgggaggtgt gatgtttgag tcgctggatg aacggctgat gatccgccac
    49981 ctcccaggcg tcttctgtgc cggggaaatg cttgattggg aagcaccaac cggcggctat
    50041 ctcttgaccg cctgcttcgc cagcggccgc gttgccggca ccggtgctgt ggattggctc
    50101 aaagccgcca ggcacagaag gtaatcagct ccttcatgca ttattcccgt cgttctgaaa
    50161 aagatcgctt ctctacaatt caagttcacc agtgacaaat tgagtcgtca ggtaaatggg
    50221 aaatggatgc atttgcgcat cttgccgttg attcttctat gcgggagaag tagcgtagcg
    50281 cagatacgac ctgtacttcc tgttcctgtt caggaggcta ccatatgatg cgtgcgattc
    50341 agcttggcac tgtggtttca atcgtggggc tgctcctcgc tgcttgcagt ctggatgtat
    50401 tcacgtctga agttgcctgc gtccagacat tcatctctgg agacattcaa catgtggggc
    50461 aactaagaac tgaacgattc ctgggcaagg tatcggagac cagagctcga tgcttgggag
    50521 ggcagtatgc cgtggattat cggtcaggtc cttggctgga ctggccaaac tattgggcaa
    50581 cccgtgatgc atcttcccgc gtccccttga gtctcttctc tgacgccaag ctgatcggcc
    50641 ccaacgctca cgggatcaat gccgccctct atgaattgga gcttcagcgc attgagctca
    50701 tcaagttcaa tttgttcgat aacaacaaga cctatgaagc gtatgtcaaa gggcgaggag
    50761 acgatccggg tccgattctg aaaacctggc cggaaatgcg actggccgag gcgcaccata
    50821 attataaaga cgtcggtgga gcggcacccc aacagatctg cacgggagac acaatccgtt
    50881 ttcgaagcgt caccggtatc tgtaacgata tctacaatcc cctcatggga tcgacgcatc
    50941 agctgtttgc gcggaacgct gagtttgaaa caacgttccc ggatctggga ctggaggaga
    51001 tcacgaggaa tcggcatggc gatcgcctcg gtctgctgaa gcctgatcct caggtgatca
    51061 gtcgaaagct cttcacacgg aatgaatcgc atcccgagtc ctgccacaac ggctatggag
    51121 atgggaccgc ccaaacagac tgcgagtacc ggaaagcccc gttcttcaac gtactggcgg
    51181 ccttttggat tcaatttatg acgcacgatt ggttctccca cctcgaggaa gggcacaacc
    51241 aaccgtcatg gatggatgtc ggctgtcaga agcagttggt cggtaacgtt gaacagccgt
    51301 tgagtgaagc ggacatcaag cggttgggct gccggcccgg tgatcggatt gatcgagcct
    51361 atgtggtgga agaggtggag ccgccaacct ttacccacga tggccagacc tacctggtac
    51421 gggccccgaa aacaatgcgg aacaatgtga cagcctggtg ggatgcgtcg cagatatacg
    51481 gatacgatga catctcgcgc aagcgtgtga aacgcgacgg caaggatccg gcaaaattac
    51541 tcttggagcc cggaaaggat ggcggctacc tgccggtgtt taccgtaaac gatccgatca
    51601 atcccgaatg gtcggggcag gaagccacgg cctttcctga taactggtcg atcggcttaa
    51661 gcttctacca caacctcttt gcgcgcgaac acaatgcctt cgttgatgcg tttcgcaagc
    51721 agccgggaga ggccgatagc ggattgcggg atcctgccag gccggatgag gttattctgt
    51781 ataagaatgt cacgaatgat gagctgttcg aaattgcccg tctcgtggtc tctgcggaga
    51841 tcgccaagat ccatacgacc gaatggacaa cgcaattgct ctacggagaa cctctctata
    51901 aagggatgaa tgccaactgg cacggtctgt tgcgcgacga ttccgatgtg tcggatgcct
    51961 tgaaggaagt cgtccgccgg ctggatgatt cggacaatcc aaaaaaatca aacggcctct
    52021 acgcggcact ggccggcggg ccagggattt tcggcctggg caatcgggtc tatgaggaac
    52081 agagcgtcct ctccattccg tttggtgaaa agaaggatgt ctggagcctg aagaatcccg
    52141 aacatgttaa cggtggcgtg aagcatttcg ggtccccgtt taactttccg gaagaattta
    52201 tcaccgtgta ccgcctgcat cctctcctgc cggacatgat cgaataccgt gaatggagcg
    52261 ctgatgcgaa cgcgatcaag gcaaaggtgc cagttgtgga gacgttccgg ggcagggcca
    52321 ccggcgccat gcggcaaaaa ggtttggcga attggacgct cagcatgggg cgccagcgac
    52381 tcgggctgct caccttagag aaccacccac agtttttgca aaatcttaag atgggtcggc
    52441 tggagtccgt cacaaaacag atcgatgtgc cggcgttgga cattattcga gaccgggaac
    52501 gaggcattcc ccgctttaat gagtttcgac gacagtacgg cttgaaacaa ctgacgagtt
    52561 tcgacgattt tatcgatcag cgcgagaaga attcgcaaga gcgggcaagg caggaacgac
    52621 tcatcaacac gcttcgcgag gtgtacgggc aacacccatg cgacgaatcg aaggtcatca
    52681 ctcacgcgca gttggatgat cacggcaagt cgatcaccga ttgtttggga cattcgaacg
    52741 gcactctggt tgataatgtc gaagatctcg atacggtcgt gggatggctc gcggaattca
    52801 gacggccgca cggtttcgcg atttctgaaa cgcagtttct cgtcttcgtg ttgaatgcgt
    52861 cgcgccgcct gttcagcgat cggttcttta cgtcgagctt tcgtccggag ttctacacgc
    52921 gatttggagt gggttgggtg atgaacaacg ggccggatgt tcggatggaa gcagtgccct
    52981 ccaacggcca tcgtgagccg gtttccccca tgaaacgggt cttgctcagg aacgtgccgg
    53041 aactggcgcc tgagctggaa tctgtcgtca atgtgttcga cccctgggcg cgggacagag
    53101 gcgaatacta ctcgttggat tggaggcccc gacctgatgc aaagacggat gcggcgtttg
    53161 ccctacagta gtgcggggga tgaggagcaa ccccgattga ggatcgctgg acgagggttt
    53221 aattggaact gagcttgata ccgctgacgg caccgatctg ccgatacttc cgatcgcgtt
    53281 gggcgagaag ttgctcggct gaaagctcat tgagctggga tagctgattc gcgagactct
    53341 tggcgactcg ctcggtaacc gctttcggct cgcgatgggc tccgccgagg ggttcggaaa
    53401 tgacttcatc gacgattttc agggctatca aatcctgagc ggtcattttc aatgcagccg
    53461 cggcatcggg tactttcgcc ggatcgtccc acaggatggc cgcgcagcct tcaggagaaa
    53521 tcaccgaata gaccccatgc tcgagcatca gaatgcgatc agtcacgccg agggctagcg
    53581 cgcctccgct gccgccttcg ccgatgacga gcgaaatgat gggaacggtg agacgtgaca
    53641 tcgcgaaaag atttctggcg atcgcttccg cctgcccgcg ctcctcggcg ccgatgccag
    53701 gataagcacc cggcgtatcg atgaaggtga tgatcggccg gccgaatttc tccgccatct
    53761 tcatcaggcg caaggctttc cgatatcctt ccggattcgg catgccgaaa ttccgctgca
    53821 tccgctcttt caacgtcttg cccttttggt gaccgatcac catcaccgaa cgatcgttga
    53881 atcgggcgaa tccgccgaca atagcgcggt catcgccgaa cgagcggtcg ccgtgaaatt
    53941 caagaaaatc gcggaagatt ccaccgatat aatcgagggt gctggggcgt tgggggtgtc
    54001 gagccagttg ggttcgctgc cagggggtga ggttgctgta gagttcgtgt tcgagttggg
    54061 ccagtttcgt ccgcagcttg cggatgtcgt cctgggtact ggacttcgtg ccgccggcag
    54121 ccgcgagctt ttcgatcttt tcttcgatct cgcggagggg tttttcaaac tcgaggtaat
    54181 cgcgcataga ggttgtggtc tggcattgcg gatcggggga ggggagatag acaccacacc
    54241 ccggatacgt agcgctacga taccagagaa agggcccctt tgcctagcac ttcttcaaca
    54301 tccgctacga agtgttcact ggcgttgacg ctcagattgg gaaggggggc ggtttcggct
    54361 tccagggttc caccggtacg gaatgtcagc gaaacggacg tgcccccggg atgacgtttg
    54421 aagacatcca ggagccttgg cagctgtgcc atgacctccg gtctgtcgga caaccgaatg
    54481 tgaatgcgtt tgatggtttg tgtctggacg tcggcaagag gctcgatctt actgccgcgg
    54541 atcttcgtac ccttgtctcc ccggtcgatg gtaccggtga tacggacgag cctctccggt
    54601 gcaatgagat cgccggcggt tttgaacagg tccggaaaca cgatcacctc gacggtgccc
    54661 agtaaatcct ccaggcctag ataggccatg cgatcgccct ttttcgtgag catggttttc
    54721 accgtggcga tgatgccgca gagtttcact tctttgccgt ctgaaagttc tgcgagtccg
    54781 gccgtcgtgg cagaggccag ggcgtggatc gtggcttcgt agcgggccaa cggatgggct
    54841 gtgatataga acccggtcag ttcccgttcg tatttcagcc gttgcgcctg gtcccactcc
    54901 gggatattcg gcaacgccgg ttccatggct gaggcctggt gtcctgcacc gttcagttca
    54961 tcgccgaaga tactcgtctg gcctaattcg cgttctcgct gcgctgccgc cccatcttca
    55021 gtggcctgat cgagcacggc catcagctgc gatcgcttcg cacccagcga gtcgaaagcg
    55081 ccggccttga tcaacccttc cagcatccgt ttgttcacct tgtggagatc gacgcggcga
    55141 cagaagtcga agaaggactt aaaagggccg gattcggcac ggattgccag cacggactcg
    55201 accgcgcctt cgcccacatt cttgattgct gccaagccga aacggatcgc tccgtcgacc
    55261 acggcaaaat tcttctgact ctcgttcacg tccggtccca ggactttaat gcccagatcg
    55321 cggcattcgg taaagtagcc gacgatcttg tcctggttgc ccatgtcggt cgtcatgagc
    55381 gctgccatga actcggtcgg atagtgggcc ttcaaatagc cggtgtggta gcagaccacc
    55441 gcataggcgg cggcatgcga cttgttgaaa ccatacccgg cgaacttctg gatcaactca
    55501 tagagctttt cggctttctt ctccggaatc ttgttggcct tggcgccttc aaggaatttg
    55561 acgcgcagtt tttccatctc ctccggtttc ttcttgccca tcgcgcggcg gaggatgtcg
    55621 gcttgtccga gggaaaaccc ggcgatcttg ttggcgatcg ccatgacctg ttcttggtac
    55681 acgataacgc cataggtgtc cttcaggatc ggttccagtt ccggtgtttc ataagtgatc
    55741 ggcaccttcc cctgtttgcg cttgatgaag tcggggatca ggtccatcgg gccaggccga
    55801 tacagagcga tgatggcgat gatgtcctcg aacctgtccg gcttgaagcc ggtcaggagg
    55861 tcgcgcatcc cggagctttc cagctggaag acgccggtgg ttttgccgga ggagaggagt
    55921 gcgaatgttt gaggatcgtc aaacggcagc tgatcaacag acagaggtgg ttcgtcggga
    55981 tggctctcat tgatgagcgt ttccgcccgc cggaccatcg tgagagtttt gagccccaga
    56041 aaatcgaact tgaccaggcc gattttttca acgtcaccca tcgagtattg cgtgacgatt
    56101 tcgtcgttcg cccctttata gaggggaacg tgatccgtga gcggctcttc ggagatgacg
    56161 accccggcag cgtgagtcga cgcgtgacgg gccaatccct ccagcgactg agcgacggtc
    56221 atcagctcat tgaccttcat gtcggtctcg accagctcgc gcaggcgggg ttccgtgtcc
    56281 aaagcctgtt gcagggtgat gttcaattga ttgggcacca gcttggcgac tttgtccgca
    56341 tcggcatagg gcatttcgag cacacgtcct acgtcacgga tcgccgcttt ggcgccgagg
    56401 gtgccaaacg taataatttg agcaacgtga tcggacccgt acttgtcgac cacatagtta
    56461 atgacttccc ctcgccgatc catgcagaaa tccatgtcga tgtcggggag cgagacccgt
    56521 tcggggttga gaaaccgctc gaagagcagg gtgtagacga gcgggtccag atcagtaatg
    56581 cgaagcgcat aggccacgag gctgcctgcg gcggagccac ggccgggacc gacggggatg
    56641 ctgcgcgagc gggcgaatcg aatgatgtcc cagacgatca ggaaatatcc cgcaaatccc
    56701 atcgagcaga tgaccatcaa ttcttcacgc agccgctgtt catagacggc ggaagggatt
    56761 ttgctcggcc ggtctttcaa gcggtccttc aatccctcga tggccagatg ttcgacgtag
    56821 gattcgcggg tatagccatc cgggactttg tactgaggga gatgggtctt gttcagggcc
    56881 agttcgagat cgcaatagtc ggcgattctg caggtgttgg tcacagcatc aggaaattcg
    56941 gcaaaggctg aggcgatttc ttccgtcgat ttgacataga gctgatccgt atcgaacttc
    57001 atccggttcg ggtcgctgat ggttttgcct gtttggagac agagcatcag ctcgtgcggc
    57061 cgggagtctt ccttcttgag gtagtggcaa tcgttagtgc cggccaaagg gatccccagt
    57121 ttcttgtgga tttccatcaa cccggcgttg gcgacgcgtt gatgatcgag gccattggcc
    57181 tgcacttcga gatagaaatg atccttcccg aatatctcct gaaattcccc tgcaacagcc
    57241 atggcaccgg ccatgtcttt ctgaccgatc agataaggaa tctcaccgct gaggcatccg
    57301 gataaggcga tgagtccttc atggtgttct ttgagaatat ctttgtccat ccggggctta
    57361 taatagaatc cttcaaggta cgctttacta acaagcttga tgagattctg atagcccgtg
    57421 aggtttcgag ccaggagaat gagatggtag tagtcgttgt gcgacaggcc gctgtctttt
    57481 ttcgccaagc ggctgcctaa agccatatag gcttcgcagc cgatgatcgg cttcactcct
    57541 gcttccttgg ctttgcgata gaactcgatc gcgccgaaca tgttgccgtg gtcggtcatg
    57601 gccacggccg gttggccgaa cgccttgatt tgctgcacca gcggctcgat ctgattggcg
    57661 ccgtcgag
//
LOCUS       DATIFR010000134        86637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2387279, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000134 DATIFR010000000
VERSION     DATIFR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 86637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86637
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387279"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VLD60_07990"
     CDS             <1..528
                     /locus_tag="VLD60_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HSB44936.1"
                     /translation="KKMSKSVGNYIALEDKPGELFGKVMSISDALMFRYYELLTTEDL
                     AKVKVLHPMEAKQALAQLLVTRYHGEPAGKEARGGFQQKFQEREFPSEPDVRLILTSA
                     DLKEGKGISLADLVAKTGLVPSKSEARRLIIQGGLEVNDQKQTDANAVLNLVAGQAYR
                     LKIGRRKFALVELQG"
     gene            complement(586..1371)
                     /locus_tag="VLD60_07995"
     CDS             complement(586..1371)
                     /locus_tag="VLD60_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB44937.1"
                     /translation="MAKNLIRAGGTVLNNCTFPAGTFSRYIAIFTKSWLRLTCLMILI
                     FYMSASVVCNKVYAQDTLSKEELAAQGVIVVKPEPGQRGIVLGLRAGAAIPTSKVLEN
                     VGNGTSVGPLVNAEALYTLNDWIRVGMMLEWHQHSIDLWGPQLGTLSVFSILPTVEFR
                     PTRETRENLGLGSFIPYVSLGTGVNVHSFSNDNQTANNADSFSSTFALRLATGFDYPI
                     TSRWAFNTELAWNRDSGTYELNGREADFDASTLNILVGVRAYF"
     gene            1678..1752
                     /locus_tag="VLD60_08000"
     tRNA            1678..1752
                     /locus_tag="VLD60_08000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1710..1712,aa:Gly,seq:ccc)
     gene            2309..2623
                     /locus_tag="VLD60_08005"
     CDS             2309..2623
                     /locus_tag="VLD60_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44938.1"
                     /translation="MTRLQFVLVLVTTFVGSVVGGVLSERILSVGSVHAQKANGVNAE
                     EFLLIDQNGKARAGLGLDPAGEVGLVMTSRDGSRKLALSPDGPVVLKISDRSGRVLWS
                     AQ"
     gene            complement(2646..2837)
                     /gene="rpmB"
                     /locus_tag="VLD60_08010"
     CDS             complement(2646..2837)
                     /gene="rpmB"
                     /locus_tag="VLD60_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSB44939.1"
                     /translation="MAFVCDICSKKHQSGHNVSHANNKTKRTFNPNLQRVRAVVDGST
                     KRIRVCTRCLRSGKVTKAV"
     gene            complement(3277..4707)
                     /locus_tag="VLD60_08015"
     CDS             complement(3277..4707)
                     /locus_tag="VLD60_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44940.1"
                     /translation="MKHLIIAAAVFWALFQVTGMFWSAKGALPSGGFARLSEGPQLFI
                     NNPYHNGYFYLFGLTAAAPLDPAKAGYEIWVEEFSGSHHAGNVENRPSRSELSFTISP
                     EFSSPSWESDEPLTEFRKQQASLRAATGEHQILIARYERWLGMPFEDWGFSHRVTPRY
                     QAIMAVHRLYISEGFSLGITEGLDRLNREFQFWRVVLREAKTIDTKMFSQILISDDLS
                     LLSRILAKPTVDKAFLTMGLQLTLPLSGSEHSLRWPIRNQLALAVKEGRAGRIMKDSR
                     SEQAKADEEWLLTSANLPLQAFDRIEHPPARTWFGLTLQSAQAWETYAAYYDALISAS
                     ENGAKRLPAMHEVTGTLQRGMIENLLNPYPVAPDWETFHRKLMETDTRLRLASLQIQL
                     RRPSAQTAVPTRLAQVGSQYFDPFSGLPMLWSPTQQKLYSVGKDRLDDGGDPTFDISV
                     PAIVVATQTAPKEMGQSFRSPRSTRQ"
     regulatory      4930..5108
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5303..6196
                     /locus_tag="VLD60_08020"
     CDS             5303..6196
                     /locus_tag="VLD60_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSB44941.1"
                     /translation="MKRRQQGILTGMPFMANVSARAYVDDAGRRLYLAKPPARIVSLA
                     PSITEMLFAMEAGDQIVGVTDFCDYPPEALKKPKVGYSNPNLESLVALQPDLVVAPND
                     FLKPDVIVRLEQLKIPVFILADKNVDDIFVHIQTLGRIVGRSPKADAVAMDLRRQVAA
                     IKQRVQRMVPVRVLYVLNSQPLITVGPGSFIDQLIGMAGGMNVAAKSATPYPRFSLEA
                     VLQEDPEVLVFPVGKAEGIPESEQQTWLQWSTMTAVKRGRLHQIPADWLNRPGPRIAK
                     GLESLAAILHPDSAIDSRVHR"
     gene            6209..7285
                     /locus_tag="VLD60_08025"
     CDS             6209..7285
                     /locus_tag="VLD60_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSB44942.1"
                     /translation="MVDLVSSWANAGSVLTVSRWVTVIGGLLLASLAVLVLCLQFGAT
                     SVDVRTVATAITHMFRGDESGSPSLRTSEIIMVQIRFPRVLMGFLVGASLAAVGVTLQ
                     ALLRNPLADPYVLGVSSGSALGVSLAILFGIGSAVMLLPLLPIFGFAGGLLALLLIYR
                     LAQSKGRLPIHSLLLAGVILNAILTAFIMFITSIMEPNRSAGLMAWLMGSLTAPGYPA
                     LGVFSMYVVLVLAILMHKAQLLNVLTLGEETARSLGVETEGVKKQLFALTALLTGAIV
                     SVSGMIGFVGMVVPHAVRLLIGSDHRLLLPASALVGGMFLAVADTLARTIMAPAEIPV
                     GIVTALAGGPFFLYLLLCRKDRML"
     gene            7287..8162
                     /locus_tag="VLD60_08030"
     CDS             7287..8162
                     /locus_tag="VLD60_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB44943.1"
                     /translation="MTAARTGTAQSSLAVNPPAIEVEGLSFRYHTREEQGRKWTLDHL
                     SFHVDAGEILGIVGPNGSGKSSLLKVCTGLLPAGEGQVRLGGVPLRKQSQSDIARMVA
                     LVPQEYVQVFPFTVAETVLMGRFPHRTARWWSLGIGDETANDLACAHQAMVETDVLPL
                     ADRLVSDLSGGERQRVMIARALAQEPNILLLDEPTVFLDINHQIEICSLIAQLRAERR
                     LTVVLVSHDLNVASQYCDRVLMLKEGRLCRIGPPDETICPDILRMVYGCDVVVDAHPQ
                     TGRPRVTMPISVTRF"
     regulatory      8214..8406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8435..10465
                     /locus_tag="VLD60_08035"
     CDS             8435..10465
                     /locus_tag="VLD60_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB44944.1"
                     /translation="MTRCSRFCRVWWCLLPLFALSYPVHAAQEIEVASADQTDIIETR
                     EVVVSATKTPVPVAQLTSAVEVITGEELERKKIKTVIDALRLAEGVFATSSGGPGTEA
                     SVKMRGAAAKHTMVLIDGVIVNSPTTGVYDFANLTTENIDRIEIVRGAQSMLYGSDAM
                     GGVINIFTKRGVDRFTSSAFVEYGSFASIREGVQASGAKGPFDFSASLSRWDTSSFSA
                     INYRRGAYERDGFHNWQASGKLGISLPKDGRLEFNLRWYNSDLNFDGFADSGAPADVF
                     GAKQTNRNLIISGTYDQPITSWWTQKLTLAQANEQLLSQSGTAGYNVNTGQNITANPN
                     SCFPNFDTCFTPFSTNLDTLNQRLEWQHNFQVRKELLLSAGYQFRQEQGDASGFFGSA
                     SPKKLISSNAGFAQAQVNLFDRVLFTGGVRQDSYNVFGDATTYRLTGGYLHKETGTKI
                     RASYGTGFKAPTLNDLFFQGFGNPNLQPEKSKSADVAVDQSLLQDKLNLSVGYFWNHF
                     NNLITFLSVPSPNCPASSFGFCPINIASARTEGWEMSFKFELMKGLDFRGQYTMTVTR
                     AYGVPGRPENTRLPRWPYDQATLGVSYQPIDVARINVDYRFVGARNNNADNTPSQRQG
                     SFGVVNASATYDVTKNWQVFGRVDNLLNQKYEEILYFGTPIRSVYGGVKFTY"
     gene            10482..11864
                     /locus_tag="VLD60_08040"
     CDS             10482..11864
                     /locus_tag="VLD60_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HSB44945.1"
                     /translation="MRFPRLVIAGTHSGVGKTTVTLAVLAALKHRGRQVQPFKSGPDF
                     IDPGHHSAATGRPSRNLDGWMLGEAVNRAIFVRAAADADISVIEGMMGLFDGSSPVSE
                     IGSTAELAKQLAAPVLLVIDGSTLARSAAAMASGYAKFDSNLRVVGVLFNRIASEGHY
                     RLLKEAVEAETNLAVAGYLRPDPSLTVSDRHLGLVTAIEQGSIDLYERLAKVAAETLD
                     LDRVEQIARSTSELDFLPISVHGRGGQGEGVRVGVAYDSAFCFYYPENLELLEAEEAE
                     LVKFSPLNDQVLPDVDLLYLGGGYPELFGEKLAGNLAMRKAIRSFAERGGAIYAECGG
                     MMYLTQAIRDFAGLSHEMVGLFPAEAVMRQPGLTLGYRTVELTRDCILGASGTTARGH
                     EFHYSTLAPRGSLAYACALSDARGESKGQDGLAKGNVLALYTHLHFASQPGIAKALVA
                     SARQTARSVK"
     gene            11861..12460
                     /gene="cobO"
                     /locus_tag="VLD60_08045"
     CDS             11861..12460
                     /gene="cobO"
                     /locus_tag="VLD60_08045"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSB44946.1"
                     /translation="MTDQAEHKAKMERLKASVDRRIEAAQQEKGLLIVYTGAGKGKTT
                     AALGMALRCLGHGMKVAVVQFIKGAIDTAEGRALNSFGDQIAFLRMGEGYTWETQDRE
                     RDKKVAQQAWQKAAEFLRDPSYAMVILDEFNIALQHGYVEFPDVLPALRARPPMQHVV
                     ITGRGANKELTEEADLVTEMKQVKHPFRKGIKAQAGVEF"
     gene            12755..13552
                     /gene="bluB"
                     /locus_tag="VLD60_08050"
     CDS             12755..13552
                     /gene="bluB"
                     /locus_tag="VLD60_08050"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: protein motif:HMM:TIGR02476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HSB44947.1"
                     /translation="MSRQTDIVCGNGAFSDDERAAVYRVIFERRDVRRNFMQKPITDD
                     VLARILTAAHHAGSVGFMQPWDFVVIRQRATKRAVKQLFMKTNAEAAMQYEGSRATLY
                     RSLKLEGIEEAPVNISVTCSRQRGGRHVLGRSTVRDTDLYSTCCAIQNLWLAARAEGI
                     GVGWVSILDHGALKQVLGIPRSVKVLAYLCLGYVPEFAARPDLEQAGWRSRIPLEQLI
                     HSELWGNRIQGVRRNGDAHHQSLHENGGRGKNKVGRRAGSMEGQSSS"
     gene            13458..14054
                     /locus_tag="VLD60_08055"
     CDS             13458..14054
                     /locus_tag="VLD60_08055"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSB44948.1"
                     /translation="MRITRVYTRTGDAGKTKLAGGQEVWKDSLRVEAYGTLDELNAAV
                     GVARVMNAELMGSLAEAEQLEEDLRWIQNKLFDLGGILATVPGQEFKNMPQVTPRDVT
                     RLEKLIDRCQKDLQPLKEFILPGGGKVSGFLHQARTICRRAERLCVRLSREELVDPTI
                     IKYVNRLSDALFVLARWAAKTQDEPEFLWERDVKSHSK"
     gene            14032..14592
                     /gene="cobU"
                     /locus_tag="VLD60_08060"
     CDS             14032..14592
                     /gene="cobU"
                     /locus_tag="VLD60_08060"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HSB44949.1"
                     /translation="MSKATRSKGGIKHKRTGRIILVLGGAASGKSAIALRLAGMKKPK
                     AFLATGQGLDDEMATRIARHQASRGSDWKTAEVPAELATWFKQKGTAYQTIVLDCVTL
                     WLSNLHEAGVSASAVARRVDELLAAMRRTNVLIVIVTNELGLGVVPMNRAARGFRDLA
                     GRVNQQIAAAADEVHLVVAGISLRLK"
     gene            14609..15661
                     /gene="cobT"
                     /locus_tag="VLD60_08065"
     CDS             14609..15661
                     /gene="cobT"
                     /locus_tag="VLD60_08065"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HSB44950.1"
                     /translation="MSLHNLLSRIQTPDPRLLDKAQSRLDRLTKPLGSLGRLEELAAR
                     YVMMTGELKPNNPRGAVFTFAADHGVAMEGVSAYPREVTPQMVLNFLRGGAGVNVLAR
                     HVGVDVRVVDIGVAYDFGAISGLIQKKIMHGTKNLLVEPAMTRAQAEQTIEVGIELAS
                     IAAQEGIGLIGTGDMGISNTTPSAAITAVMTGRPVAEVTGRGTGIDDAGHVRKVAVIQ
                     QALDLHRPDRTDALDVLSKVGGLEIGGLAGLILGAAAERLPVVLDGFIAGAAALIAVG
                     LQPRCREYLIASHRSVERGHQAILDYLGVKPLLDLDLRLGEGTGACLGMSLVCAAIKI
                     YSEMATFDEAGVSEKE"
     gene            15667..16431
                     /locus_tag="VLD60_08070"
     CDS             15667..16431
                     /locus_tag="VLD60_08070"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSB44951.1"
                     /translation="MGAVARPFVFAWHFLTAIPLSREHHTPTAPELAASMAWYPVIGL
                     VIGGGLALIDLALARLLSPTIVNVLLIVLLVLVTRGLHQDGLADTLDGLAGGRTPAER
                     LPIMRDPRVGAIGATGLFLSLLLRYAGLMALPQALRLPAIICMPALGRWAMVMTAWSS
                     PYARAEGGLASPFLAHLSWFHVLVSTAIIALALVLGFGLLHAAVVLVISVIVLLVVWA
                     GCRIWFGGITGDTLGATNEVVEILFLLLLPLLLLMS"
     gene            16428..17384
                     /gene="cbiB"
                     /locus_tag="VLD60_08075"
     CDS             16428..17384
                     /gene="cbiB"
                     /locus_tag="VLD60_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HSB44952.1"
                     /translation="MTGGDLLIAAAIDVAVGDPRWLPHPVRGMGLAISCCDEQFHRFC
                     KSPMSLRIAGLCLAIGLPVVVYGIGWFAIELAGSVAALFGQAVGIALASTTLAWRDLW
                     DHAMAVIRELAGENIPEARQALGMIVGRDTVSLSETEIVRAAVETIAESTADGVIAPL
                     VYLTLGGAPLALAYKAINTLDSMIGHRDERYVDFGWASARLDDLANWIPARLTAGFMV
                     ITAGLATGQWERTIESWRILLRDGAKHPSPNSGRPEAAMAGALDVQLGGTNYYDGLLH
                     EHPVMGDGTRMLTPGHIRYALRIMTMTTLLGLGLAVVSLWLA"
     gene            17381..18478
                     /gene="cobD"
                     /locus_tag="VLD60_08080"
     CDS             17381..18478
                     /gene="cobD"
                     /locus_tag="VLD60_08080"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HSB44953.1"
                     /translation="MKANGHGGDVYFASRELGRDVERLIDFSASINPLGPSSQVWKAL
                     SKARIALQHYPDPACRDLRQVLAMRWHCSPEEIVVGNGSTELIHVLPSALDIRNLLIV
                     GPTFSEYASAMSLVGGRVTTICAERHQGYRPPLEGVVALLSRRKRTYRDIGAVLLCNP
                     NSPTGQACDAESVLDLARLAQRRGLWLVVDETFADYCEERSILPKRSLFSRVVVLRSF
                     TKFYGLPGLRVGYAVAAPAVVERLRSRMPPWSVNAMGQVAAVAALNDAAYVGRSLSFM
                     RNERARFIARLARLPGCNVFPSQANFVLVELPHRWGASAVAEKLRPEGLLIRDCSSVS
                     GLNTHSIRVAVRTRRENDRLVTALSKLLVHR"
     gene            18483..19229
                     /locus_tag="VLD60_08085"
     CDS             18483..19229
                     /locus_tag="VLD60_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HSB44954.1"
                     /translation="MSPRSKARLRTRYRVVEKTLVIDLGGFKRVLSSASQGGGFQSAR
                     YILNHQVEAHPVSRAHARSKRLIDPARYMRRLAMTLGVGEKPIGLMTAVPMKQLVTSC
                     KRFGDTWVECFATVGVTNAVRAGEWPERENGGKQGTDAGTINLVLVTNACLSGAALVG
                     AVQVVTESKTGVLRDHAVPSWTGESGATGTGTDVVVIACGLRGQGPWHRYSGTHTIIG
                     AMIGRVVSDCVRRGLAREQRWRRSQGSLKK"
     gene            19226..20755
                     /locus_tag="VLD60_08090"
     CDS             19226..20755
                     /locus_tag="VLD60_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HSB44955.1"
                     /translation="MKAQAVAILGTGSDVGKSLMVAGLCRLLYRGGVRVAPFKAQNMS
                     NNSFVTPDGKEIGRAQALQAHACGLDAHVDMNPILLKPESDRRAQVVVQGKVWSTSDA
                     QDYFHQHNELAQRVRESYDRLAAQYSVIVIEGAGSAAEMNLRDRDLANWFSVELADAK
                     VLLVADIDRGGVFAQVIGTLDLLSPHERDRVFGIVINKFRGDAALFADGVSFIEQRTG
                     LPVLGVVPFLRGVVLEQEDSLDIERRRHRRFAADRVNIGVVLLPRMSNFTDFDPLAAE
                     QDVILRYVAEPHELAGADVILIPGTKNTIEDLRYLRQAGFTDTLREHVAQRREVVGIC
                     GGYQMLGRSIADPEGVEAGGIAAGMGFLDVTTRLLKKKITLRIEAASLHLNDGPAPSV
                     RGYFIHMGETERGQESACFHIQSVMGVSDHQERTLGDLMEGAVSADGLVWGTYIHGIF
                     DRPAFRRTWLNRVRQRRGLPPLSVEQSEGVTQRLLAELDRWADHLEQHVNVSLIFKAM
                     D"
     gene            21683..22414
                     /locus_tag="VLD60_08095"
     CDS             21683..22414
                     /locus_tag="VLD60_08095"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSB44956.1"
                     /translation="MNATRLSWRILSGVAALALSATPVFAGNEAEVAEHLIDLVKIGR
                     GVMSEQMGNINDASKADKGFTGDYVAGQVIERFKKKTKIDLRIPNVVPQAGLYLALVE
                     AEKEVVDEAQPIINRPGIGFKGFIPAVFARKTGEHFYKKSGVRLKLTGIDYRNINNRP
                     DDFEAEVLRMFSDSRHPKGQSYVRNTMVDGKPVLRMMDPEYAAASCLACHGTPKGGKD
                     ITGGHKEGWKEGDLAGAISVVLPLK"
     gene            22478..22840
                     /locus_tag="VLD60_08100"
     CDS             22478..22840
                     /locus_tag="VLD60_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44957.1"
                     /translation="MSKIVVVDDSYAELQVIEGVLKGASHTVVSFPNTEKLEEKVVSE
                     KPDLIVLDVVMPGRNGFQACRDLKNDDRTKSIPIILCTSKGNESDKFWGQQQGANAHI
                     VKPFKGEELLAAVKRVLG"
     gene            22876..23370
                     /locus_tag="VLD60_08105"
     CDS             22876..23370
                     /locus_tag="VLD60_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSB44958.1"
                     /translation="MPSVSGTVVPSPGGGTVQATGAATVRAAIVSLGGELFTIDLQNV
                     REVFVVESITPVPGMPSGLVGVTNLRGSVVPLLDLRQMIGLAAETTLKYAVVVKHGNW
                     QVGVLVDTVPEIRTLTKDQFIPAPTGTGDGGNPFVSTVVKLEDRLRGVLETSVVLSHF
                     EGTG"
     gene            23443..25317
                     /locus_tag="VLD60_08110"
     CDS             23443..25317
                     /locus_tag="VLD60_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSB44959.1"
                     /translation="MLRLNVLQRFENLKTQAKLLTVFALVGVIIAAMAGVGIVTNSRL
                     AAQAETVYVDYTVPLTEFNTLLFNVNRFHEALIDVARTTRASDFEIEVKDLAPYRGEV
                     ERLIANYEATTLRISSTGRDEAKDLAELKDAIKKFFDQGEAAVNTIKDSYESKSLTAS
                     QALAMRDLGQLAITVSLSPAYEKVTERHAESIKTMAEVAKDLSEQSRSMAANATLILI
                     GGGIAAVLLGLALGYWVAHKLSLGIGQVASVAQQAAAGNYQARAKITSKDELGQMASS
                     FNTMLDRITALVTSEGERDEMQKRLMNFLVLVSDVGKGDLTKRGEVTADMFGNLADGF
                     NLMIARFGQLLKQVREAAERVNKSAGTLRDSAGQMAGTAKHQADESVKTLSAVEQLAA
                     QMRKVAETAGASSESAKQVLQATERGRVAVQETVQDMQSIRSAVQRMSKQVKALGDRS
                     LEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATRE
                     IAELVKVIQTETQDAVVAMEQETQAVEAGSASALRTGEVFNEISGIAQRSAELAQTIA
                     SSAATQTASTDQVGRSIKDFTGGAVATQKATDAARMTVEDMAKLADGLTASVAQFKLA
                     "
     gene            25371..28805
                     /locus_tag="VLD60_08115"
     CDS             25371..28805
                     /locus_tag="VLD60_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSB44960.1"
                     /translation="MSSELDRQGLLDIFVTEAVEALAVLAAAVNPAGEALPSAQELQN
                     QYVWAHKVRGAAGIYGFSGLSLLGELLETALENSTTIDPALWPKTVGALRGMVEAFQA
                     QLEIVRQGGQEDPTVGERWKAEMAALLPASPDTLSTEGATSDQLPPEYLVPTIDAEVL
                     SYFSPEADEYLQTLELLTNRLRENPGDAETIQSLYRTAHTLKGSAHTIGFKVIGDIAH
                     PVEACMIAVRDGQIPVSTALLTTIGEAVNVVRLLMRRDDSKLDQLQQDVSGVTQTLHQ
                     ICNGEPVGAPPPSSPAMAQDNSAPQVATAVLEPPADSTETSEGSTSSSSPLQLTDEYL
                     MPKLDPEVLSYFAPEAHEYLESLEAQLLRLEKESENSELINQLFRTAHTLKGSAYTVG
                     FQAIGDMTHYVEDFMGAVRDGRVKVLSGHTDVLLRSVDVVRLLMRRDPTLMGTLRQRF
                     VTAMQGLKQLEQPAAVETVTKAAVAQPAPAPVVNEAPDQPEADQAKAGDARAKDASGE
                     DREVIRVSRDRLERLLNLVGELVIGRGRLEQRLQVLEQLSQQVLACKGRLMDSVRSFE
                     EKHTFTLPSSGANSADGAKSGSVVGAAGFTGLSDFGSLEFDKYDDFNILARRISEVTA
                     DISESMTQLSGSIRRSHEDMSQLQQLTLGMRDEIARARMVPVGTPFTRFRRATREMAR
                     TTGKEVTLVTSGEHTEVDTGVVERLVDPLVHLVRNAVYHGIEPAALRVSKGKPAAGTI
                     YLHASHRGNAVLIEVEDDGAGLDIDKIRAKAVERGLVRAEVAKNLPEAEVIKFIFMPG
                     FSTAEQIGDQAGRGVGMDVVKRVIESMNGHIDVESIRGVGTKFTLHLPLTLLIATALM
                     VRSGGERYGIPLPSVREVTMLTTGSFQQMGERSMIQIGDEAIDVLPLQQLLSRGSASR
                     VEIGNPVVIVRTGVGPVGLAVDELLGRQEIVIKPIGSLKSLQRSCFGGATIDPEGRVV
                     LVLDPARLLSGSEKTAGSLDVTTEGTTSSDNETPIDEAPVQPAVEQKLLLIDDSLSIR
                     KFVGRMLETAGYEVDTAVDGEEGLRKASAKNYRLILTDLEMPKLNGYEVIQALRSRPQ
                     TQQTPIIVMTTRAGDKHRQMALNIGASSYIAKPVEERALIQELERWIGQESAARKA"
     gene            28859..29377
                     /locus_tag="VLD60_08120"
     CDS             28859..29377
                     /locus_tag="VLD60_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSB44961.1"
                     /translation="MSLRGHHRATTATVQTASFLVVRFGKNYCALPSQGVRGVLTMEE
                     AGRAQTVTAVGVIYQDVDLAGRFSTVVDLTRPDTRTILYSNGQSHGAIRVEEVIGMID
                     VERHQCKPLPPHFQQDERTWISGTIFFREQLALILNPEWVLGELGEEVAVGGDREKSK
                     AISHATGIGGQC"
     gene            29371..29838
                     /locus_tag="VLD60_08125"
     CDS             29371..29838
                     /locus_tag="VLD60_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSB44962.1"
                     /translation="MLRAARQQDQAVAAKKSWNVVVFSVGGMKLAARTEDVGGVAAWV
                     EPVSVPSRTPFIGAMLKREKDVMPVYDLATRLEREPEGDTLLCLVARHIDGPMAICID
                     SVIPTLHTIEISQIVPSKRMDLETLGSFTEDGRDIDILALQRLGRRRQDSMRP"
     gene            29864..31180
                     /locus_tag="VLD60_08130"
     CDS             29864..31180
                     /locus_tag="VLD60_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB44963.1"
                     /translation="MPKVLVADDSIAVRKVAERLLTEAGLGVTLAANGEEALASLAKE
                     VPDLIVSDVIMPDKSGYEVCAFVRAQSALANTPVLLISGIVNDEVTKQAESCHADGVL
                     KKPFQGTSLKDRVLELLAKKHGQKTEPPKQAAQPAPPSGAERGSRVPDEQIAASQQTL
                     AKLKQVEEELHKERQQSAQLTQRIAQLEGQVGRVKELDAIVASEREQSAQAKKAAEEL
                     APIAQRAKELEALLKQEREQAAQVKKQADDLRGQVTRLHELEELLKQEREQVAQLKKQ
                     TDDFRGQVARLQEFEAALQQERVKTAQLQQRVTDMQKSVTRVQELEAALYAERQAATQ
                     LVQQMTAMEKAVSRSQELAQQFAREQQRVEELTRRLAVSEDGSAKAKEVEKLLETERE
                     RNAVLAHRVSETEVAAQQATKRFEEMAKKLGEIAGLASQLGNGAKR"
     gene            31435..32367
                     /locus_tag="VLD60_08135"
     CDS             31435..32367
                     /locus_tag="VLD60_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44964.1"
                     /translation="MSGDTSDEFQKELVDLFVQEAHEWLQNIHVALDELQQAPPPERH
                     AVLIQTLTAGVTNLGGSAATINLPDVEQASFAALPFIEAIKDPQKNLSVQDFLSLCKQ
                     LGLIHTALTNATGVAFDAEAEVEVVEAAASLSPAEFLQSLRTLQQNQGAATQGDRNLI
                     KTMIEQMEGQVQAGVQHVGVDVIQGFLDRMSDSEDAFVQSVDAQMPNLAAKLSGLAAS
                     ADPASDVIESSLKEVMQLRTEAQQVNAGIAMTFLGGLQSLLSVVAQHRIRLASARVEA
                     VTARLGVMKELIHQWVERGRAERTAIGHVLPVNH"
     gene            32493..34586
                     /locus_tag="VLD60_08140"
     CDS             32493..34586
                     /locus_tag="VLD60_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSB44965.1"
                     /translation="MEERRASDIYRKAEEQLTAVATAIQHQDQIDLDGLSTLAAEIAD
                     SVDRDDQLVVQALAGPSGSTLVTNLINVSILATKVGIGLGYYGKELRRLTLAGLVHDV
                     GLFAVPQSVVTKSGRLTREERTLIEQHPELGYQVIRKLGGDWEWLARAVRQAHERWNG
                     KGYPNRLKSRDIGEFAQIIGAVDVFDALVTPRPYRRRFFPHEAVRELLVAERTAFPRE
                     VIKALVEQLSAYPLGTLVRLTTGEVGTVVKINTRVPLRPVVEIGGGTGQGGSHESRQL
                     DLSLLPLVAIIETIESPTVARVPFPSGIAADRRTQPVPSVSDQFASLLESLDALASAI
                     QGVVETRAPLASKGVADAQESDQGWVTPRPSGRDLADVSFEREVVGLFALEAHEWLAQ
                     IYSALKRLGEGTNGSVRPKLYGIMLQGLTNLAKSAATVHLTSIEDMASSLLPILHDVG
                     RQEPRSMATALASLQAGLDRIAAAVRHAEGGPEPEKQEESVAGEGFPDHKAERDAEIP
                     MPSAPTLLAPVMGTTGVSLLNALRELQHVRSRSVQPARDVLEPVILRAEQQPGILTVG
                     GVRRILDELDRMDERFLEEVNQRVPAVSQLLNDLRKDGTMDFVTASQLDPVVQHVEEL
                     QAIAGSVQAGMMTMFLEGLRSFLLVAAYRKAATLPQRLATVEARIQALVPMAEQWVSI
                     GRVERGAIAEILPAS"
     gene            35277..35690
                     /locus_tag="VLD60_08145"
     CDS             35277..35690
                     /locus_tag="VLD60_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44966.1"
                     /translation="MVGGIGSLGYQLVASSTYTWTTFWSSSGLLVLGACCGWGQTRYH
                     RYLLATLPEVFAARIRSAVTRNQKKRKGESSIPAIEHPGRRLVPVAYVLGGGLLLAAS
                     AWAMFYGALDMVPAVLMPWAGFYWGKLFFWRGVVS"
     gene            complement(35714..36808)
                     /gene="lpxD"
                     /locus_tag="VLD60_08150"
     CDS             complement(35714..36808)
                     /gene="lpxD"
                     /locus_tag="VLD60_08150"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSB44967.1"
                     /translation="MGSPSATTTLTLSKIQQLVGGTLHGNGGTPLFSLASLNEATPQA
                     LSFITNDKAAKNPKAIVAGALLVHRHFPEIATPQIVVQNPLLAFAQVAQTFFVRTVAP
                     RGVSDNITQGSEVTIGTNPSIWPFVTIGDRVRIGARVTLYPGVFIGDNSIVGDDCVLY
                     PNVVVREGCSLGARVIIHSGTVIGADGFGYVQHEGRHHKIPQLGGVTIEDDVELGANV
                     TIDRATLGQTVIKRGTKVDNLVQIAHNVTVGEHDIIVAQVGIAGSTTIGHHVMIGGQA
                     GLADHITIGDQVMIAARAGVNRSLEPNQIVSGAPVMPHETWMKAQAVIPRLPELRQLV
                     RSLEQRVAALESQLAQAAAPPPARNKRRRK"
     gene            complement(36815..37120)
                     /locus_tag="VLD60_08155"
     CDS             complement(36815..37120)
                     /locus_tag="VLD60_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSB44968.1"
                     /translation="MTDQAILGRIIQALAEYLKRDAASIKASHHLRDDLGLDSMAVIE
                     LLYKIEEAFNLQIPDQDLPGLTTVGAVAGYVQGRLAPAENIASSPRKKTAAKRNKKK"
     gene            complement(37165..38421)
                     /gene="fabF"
                     /locus_tag="VLD60_08160"
     CDS             complement(37165..38421)
                     /gene="fabF"
                     /locus_tag="VLD60_08160"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSB44969.1"
                     /translation="MRARVVVTGLGVVSPIGIGVSEFWKAALAGRSGISAITTFAPFS
                     MDGYRSKVAGQIHDFSPERYIDGVQAERVDRYAQFALVAAKEALVDSGLQMAKERPHR
                     VGVIVGAGMGGMVMGEREITQLYQQQRPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     FTISTACSSSAHAVGQGLHCIREGRADVVIVVGADASITPLVFAGFCSLRALSTKFND
                     RPEQASRPFDRLRDGFVMGEGAGALILESTAHAKKRHAHVYAEVAGYAATSEAYHMVI
                     PREDGEEVATTMRLALADAGATPAQVDYINAHATSTSIGDAVEAKAIRQLFKSRADKI
                     AISATKSLVGHTLGAAGAIGGIASVLAIQTGQIHPTANYDDPDPACALKGISRSIQER
                     KIKVALLNAFGFGSNNAAVVLKRYVA"
     gene            complement(38597..39688)
                     /gene="lptG"
                     /locus_tag="VLD60_08165"
     CDS             complement(38597..39688)
                     /gene="lptG"
                     /locus_tag="VLD60_08165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HSB44970.1"
                     /translation="MPILFRYLLREYAKIFFMCFAGLMTIYLVIEFFERVRRFLRHDA
                     EWLDILIYFVLKMPGIAFQIAPLAILMATLLTLGLLSRNHEITAMRSCGISLPWITSP
                     FLAFAACITLVLLLFSSIVIPLASNEAEEIRVVRIEKKPPSTALKLSQPWARVDADTL
                     LHVSSAAIGGSTLGGVQLFRFDHAFHMIEMAEAEEARFADHAWTLYRGFHRRFNPDGT
                     VTLKHFEQQSIPLTLLPDDFVGAFAGESESMTFRDILSYTSRLYQNGSQFRRLLTDYY
                     GRIAFPFVTVIMVLVGIALSLRRSGTRGGSMAIGIGQALMVGFFYWAAHSIAIAFGRG
                     GALPPMVAGWTTNTLFLSYALYLMLKVRY"
     gene            complement(39693..40784)
                     /gene="lptF"
                     /locus_tag="VLD60_08170"
     CDS             complement(39693..40784)
                     /gene="lptF"
                     /locus_tag="VLD60_08170"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HSB44971.1"
                     /translation="MLLRKLLDRYIFTELLSPFSISLGALCFVMLTRELLRLVELLVS
                     KGVGIFAVMKVFVHLLPSFLVLTLPIAGIIASITAFGRLSLDKELVAMRAAGLSLFRL
                     GRPVFLFALLVFALTLWLAQYGQPWTSTNLKRVALNLLRDQLVLALERGSFTEPIPKM
                     MIYVSDEGTEASAAGIFISDERNPDDPRIIVAQHYQVLMDSSTDQVALRLQDGVIHSR
                     PDAIDQYQFASFSSYDLRLSLNQSGYAAADERPTYDALMAQLNQSQWRDPGARRRLME
                     YYKDLAFPTASLIFCILGVPIGIVSKRSGRIGGFAVGVLVVIIYYVFNVACEFLVTTM
                     MISPFAGAWVPNGMFAIVTLVWFYNMSRQ"
     gene            41269..41487
                     /locus_tag="VLD60_08175"
     CDS             41269..41487
                     /locus_tag="VLD60_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HSB44972.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDSGEDVFVHYSALQGEGFKSLKE
                     GENVEFDIVQGAKGPQAANVMKMSIAAS"
     gene            41654..43855
                     /locus_tag="VLD60_08180"
     CDS             41654..43855
                     /locus_tag="VLD60_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44973.1"
                     /translation="MGIDRSKVLHSAQLFASKGQFDAAISEWKKLSAESPADGSIHNS
                     IGELHLKRNATVDAVNSFIQAANAFRAEGATLKAIATYKKVLKLEPSRYDMYRHLGDL
                     NAERGLLSSAVQDYLTLGKHYLKERKTKEALEIYKRIVSQDPSNLDAQQRVAELCLQE
                     NMQDEATKVYLQLGRERSAQQRYSEAKEAYQSVLRIDPNNSEAQQFIEHFEKTGGAPA
                     RPARSSASAGVGAGESPDLLAEASRRIEDKQYAGAEAILNQLLTKEPGNPQVCQLLAR
                     LHLQQGQLQVALGEYRFLAGAALRAQDYQQAESLIAEFLAVEPNSVPLLELHGELYEE
                     KGEIETAVQHYGKAVEVLLTHPEPGMPTLHEEIFEKMCSLAPDSPTVKRLSGLMSGDT
                     AAVEPATPAPAQSAPKAEFSIEGATPDDAWSRTTAVKPLQDRHSVEAGLTVESSQETQ
                     DDPATFSIKNAAPDEEWSTVAQPACATEPEKPPLSTLERFRAYMEAGQLVQAEQWLGT
                     VVNAEPDDAEARELLGQVLEAKGDGAGAALQYARALELRTAHSDSADTKQLSGLYDKV
                     KELAPASPIVAKLAGMFAPPAPAAAPPASEQAADALDPETHYTLGVAYKNMGLLEEAK
                     EEFVLSMKGSEVFVDSCLMMAVCLKEQEQFEAACAQLERLLTDPRSQGAKAQAIRYEL
                     GLLYEKQEQWQLATTMFETIPTFHDVPQRLSSIRAVPQSPAASAKVFRYAN"
     gene            complement(43852..44826)
                     /locus_tag="VLD60_08185"
     CDS             complement(43852..44826)
                     /locus_tag="VLD60_08185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSB44974.1"
                     /translation="MTAQKPLLYVSRLLPDPVMAMIREQFTLAQEPSDAPATAPALRD
                     GMMEADAAIVTLTDRIDAETIDAAPRLKVLANYAVGYNNIDLAAANRRGLIVTNTPDV
                     LTDATADLTWALILATARRVVEGDRLVRSGQWTGWAPTQLLGAAVAGKTLGIIGMGRI
                     GQAVAQRAAGFRMKVLYHARRPAVPPPASASWERRTLPELLSASDFVSIHVPLTADTH
                     HLIGMRELAQMRRSAIVINTARGATIDEGALVAALRNGSIAGAGLDVYEKEPALHPEL
                     VSLPHVVLLPHLGSATMAARVEMGMICLNNIEAVLGGRLAPNRVDVNA"
     gene            44978..47806
                     /locus_tag="VLD60_08190"
     CDS             44978..47806
                     /locus_tag="VLD60_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HSB44975.1"
                     /translation="MLIMPDISRAVASDLRRLLGSDFVKDDEATKTAYAVDASIYRMT
                     PQAVVLVESEEHIQTVIRYAVSRGVGLTARAAGTNLTGSAIGSGIILDVSRLHRIIEV
                     NREERWARVQPGIVLAELNKQLAPQGLMFGPDPSSGEMCKLGGMLANNSAGPHTLRYG
                     AVKDNVEALRVCLHSGEWVDARDYAVDDPRLSALLSSHAALQETWSLVHAQQALLEQK
                     RPTVSKNSCGYNLFGLAEGLMRGRFDLPKLFVGSEGTLGLFSEATIKLVEKPRASLTA
                     LIHFKRLEEVGDAVPKLLELAPSALEVMDANTLDLIGRARHGIPTDAAATLLAELDTG
                     GLQIDLRAQAERMAALCRGYQLSSEVVIAYDQEQRDQLWKARKALYPTLYRFDSRKKP
                     INFVDDVVVRAERISELMRYLEDFFHGQRVPVAIFGHIGNGNAHIVPLLDVNNEQDFQ
                     KMVQGYHEIHQTVLDRFGGSICGEHGDGRVRAEYVSKMFGPELYALFVRVKRSFDPAG
                     VLNPGIKISDRPFTEHIDYTRLSKACATCAKCNAVCPVYDVFRSEDMSSRGWFEIVTD
                     KNYSYLQSKRVVEACLNCKSCRTACPAGVDVSQLILDRRAEHPNKLAGLIFAFHAQSM
                     LFGIFLKLLAIFQPIWDRPGIRRFLDIATKPILRHLAETARWSPQLVMPTLARRHLRD
                     RYAGLVPQVGLTPRSKVAYFHGCAANYFDDGVGDAVIAVLRRHGVEPDLPPQRCSGTP
                     IETYGHRELAREGARVNLQSLAGYETVVTGCASCTLALRDYPALFAGEPEQQAAQALA
                     TKVKHISEFVAQSPVKPPMAGVASTTGTVTYHSSCHLRAAGVTKEPRQLLQSLPGVEY
                     VEMPDADRCAGGAGTYLVKDFDSSQRIVERKRRAVEQSGAGMVATSCPACMIQLRTGL
                     HGAARVKHIAQLLQEAYDAADPKKAMTL"
     gene            47803..48045
                     /locus_tag="VLD60_08195"
     CDS             47803..48045
                     /locus_tag="VLD60_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44976.1"
                     /translation="MKLRKADWLFVLFAVVVVAAVSMLPTPKDRNPMIPASAEHRAIT
                     TEKECAYCHQADGVKPVPARHPKRQDCFRCHARAST"
     gene            48168..48446
                     /locus_tag="VLD60_08200"
     CDS             48168..48446
                     /locus_tag="VLD60_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSB44977.1"
                     /translation="MVTVLIFGLTLRNAVGESELDVELSGVTTVKKLVEANQDRLGGL
                     APYMANREVLITVNKKVSTDDTAVKSGDVVKFSFQSRTSYDGTRDIPT"
     gene            complement(48463..49095)
                     /locus_tag="VLD60_08205"
     CDS             complement(48463..49095)
                     /locus_tag="VLD60_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44978.1"
                     /translation="MRSLRSAFLILAIATIPAIGLAELPDFGPPDAGVPFDDIDDATL
                     QAEVTKQALAFLDNLHSHWGETPDRLRTTVAGEIERDEQGTLIYLRNISDHGVLEGYE
                     FKRESLVRGQYIVLQRPLNGLNEFIGYYEALKRTLIETYGIPLHDRIVWDNDLYQPVP
                     DYWGVAVMIGHLHYHAAWETAEGTLTLELTGNRHSKLFLEYKSRVEGAQT"
     gene            complement(49153..51678)
                     /gene="hrpB"
                     /locus_tag="VLD60_08210"
     CDS             complement(49153..51678)
                     /gene="hrpB"
                     /locus_tag="VLD60_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HSB44979.1"
                     /translation="MNRLPIEDVLPRLRDTLTAGRNALLTAPPGAGKTTRVPLALFDA
                     PWLEGKKLLLLEPRRLAARAAAHRMASTLQEPVGATVGYRMRLDTKVSSTTRIEVVTE
                     GILTRLLQQDPSLNGYGAVLFDEFHERSLQADTGLALCLETQRFFRPTLRLLVMSATL
                     DCGPVGDLLGGAPVITCEGRMFPVETRYLDQPLSGHLDVAVTQIIRRSLAQDQGSLLV
                     FLPGMAEIRRVERKLLDANLSPTIHLAPLHGDLPQAAQDAAIAPAAPGTRKVVLATSI
                     AETSLTIDGVRVVIDAGLLRVPRFDPRSGLTRLETIRVTQDSAEQRRGRAGRLEPGIC
                     YRLWTEKEQASLTARRPPEMLDADLAPLVLELALWGTSDPSELSWLTSPPSGSVAQAR
                     DLLTRLGALDLTGRITPRGKQMAELPLHPRLSHMLLKAGSPQSTTLACDIAALLSERD
                     ILRGPSGWRNADLRLRLDVLHGQPDHAAGATVDRGACQRVKRTAKLWRQQLGRVMKPA
                     KPVAHDHVDDTGRLLALAYPDRIAQRQAGTEARYLLTNGRGALFASPDPLASEPYLVI
                     ADLEGGTQWARIDLAAPIGLKDIESLYAGDIVQSEAVAWDDRAKAVLASRQRRLGALV
                     LSEQALSKPDPSSIATALLQGVRRAGLGALAWTPELQQWRARVQFLRRVEGDAATWPD
                     LSDEALLQTLDQWLGPYLEGITTLDRVKRLDLSTPLHAILTWEQQRQLERQAPTHLTV
                     PSGSHVRLEYNTSDLPVLAVRLQEMFGCKETPRVAGGQVPVMLHLLSPAKRPVQVTKD
                     LASFWANAYQGVRKELRGRYPKHHWPDDPLAAPPTAKAKSHSR"
     gene            51732..52385
                     /locus_tag="VLD60_08215"
     CDS             51732..52385
                     /locus_tag="VLD60_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44980.1"
                     /translation="MARATKSMVDVQQVIEHIRNAFRNTVHPGDAFLQGSQEGCEPGE
                     AIAPFKGLAHWMQVDPAILDPAYTALSFLSEGGFRHFLPAFLIADLEGRLQTADPVFH
                     LTNGFSDKVVKLPAGTRVYEKTIGKSAFVNPRRYGAMTWQDHARGQLSIFAREEACAI
                     VAYLEYKRDADPHGLNCDEINAALDGFWRDRAKQAPTQEALRQYLQSEEEYFKNLRV"
     gene            52413..52610
                     /locus_tag="VLD60_08220"
     CDS             52413..52610
                     /locus_tag="VLD60_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44981.1"
                     /translation="MAMYQWTTTKPTEAGWYWFRGQAHEADPFIVQVDEVGQFQWPDG
                     GYQEVKLAKGEWAGPIEEPGE"
     gene            53209..54672
                     /gene="zwf"
                     /locus_tag="VLD60_08225"
     CDS             53209..54672
                     /gene="zwf"
                     /locus_tag="VLD60_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSB44982.1"
                     /translation="MREQPVEPHVFVIVGGTGDLTHRKLLPALYHLRGQGVLEKQNTL
                     IVGAALPEMSEEGYRLWAYEGLQKSGVRHDKELRAWCEACIHYQSVHEGKPKDFEALA
                     AFIRRLERSRSMPENRIFYLALPPEVVPSTMERLDHTGLLKSHGWVRVVFEKPFGHDF
                     HSARALNTTLHRYLDESQIYRIDHYLGKETVQNLLAFRFANPIFESLWKRDAIESIEI
                     TVAEDLGVEHRGAYYQQAGALRDMVQNHLTQLMTVVAMEVPVSFDAVAIRSEKLKVLH
                     SIAPINPHDVVFGQYTAWRSGSVNIPGYLEEKGIPKDSTTETFVALKAEIHNWRWKGV
                     MFYLRTGKRLPRKLTQIAVTFHEAPTQVFRSLDPGSIPPNKLLVTLQPSEGFALCFSV
                     KTPGRPFRFTDHALQFSYEEAFGELPEAYETLLRDVMIGDQTLFVSAEFTETAWRLYD
                     PVIVGEHTVHNYTAATWGPREADMLLERNGHRWQLGW"
     gene            54725..55408
                     /locus_tag="VLD60_08230"
     CDS             54725..55408
                     /locus_tag="VLD60_08230"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HSB44983.1"
                     /translation="MGRSMGDPIQRLMGLMFKAHPWHGVSIGDKAPEVVTAYIEIVPT
                     DTVKYEVDKDSGFLKVDRPQRFSNFCPVYYGLVPQTYCGEKVAGLFSKRAKRKGMVGD
                     RDPLDICVLTEKTIPHSDILLTAIPIGGFSLADGGEADDKIIAVMKEDAAYGDFTDIG
                     DCPITLIDRLQHYFLTYKQAPGAVQHKVEITSVYGRDEALKVIRASHADYREKFPELE
                     SLWPKQMAT"
     gene            55436..57184
                     /locus_tag="VLD60_08235"
     CDS             55436..57184
                     /locus_tag="VLD60_08235"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB44984.1"
                     /translation="MKHDQNHPPAATAIHGFSPGFAALGLEASLLTTLETLGYEEPTP
                     IQREAIPPLLAGKDLLGQAATGTGKTAAFTLPLLQRIAHGAKAKPAALILVPTRELAI
                     QVSEAVQRYSKELRTSVLAIYGGQAIGPQFHALKRGVDVVVATPGRALDHIRRQTLKL
                     IHVQIVVLDEADEMLDMGFAEDLDAILEQTPATKQTALFSATMPTRIKSIAHRHLKQA
                     VEITIAKEPVKAGAAPRVQQTAYVVTRQHRGAALVRILDMAGPKSALVFCRTRVEVDD
                     VTAMLNSRGHRAEAIHGGMSQPQRDRVMSAFRSGQTELLVATDVAARGLDIPSVSHVI
                     NYDLPSSAEVYVHRIGRTGRAGREGAAITILDPREQRLLRSIEQHTKARVTVAPVPSV
                     ADLLAKRLERTRALVQEAMQADDLDGFKGVVKAMAASGDPITVAAAAMKLIFQEQGGE
                     RVEQEIPAMPIPQPEPYRPMQRLTGRPDDRYRGAQARSGGPQPMARPGRGGREPGMAR
                     VYVGAGREAGIRPGDLVGAIANEAKVNSSMIGAIQIEDRFSVVDVPEAMAQRIVEVLG
                     RARIKGRKAAVRLFRE"
     gene            57483..58217
                     /locus_tag="VLD60_08240"
     CDS             57483..58217
                     /locus_tag="VLD60_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB44985.1"
                     /translation="MPIQTFTAQVDAIKDLTHDVRQLDLRLIEPNTISFKPGQFVSFE
                     IPQATPGRSVTRPYSIVSQPSRSGGITLLFNLVPGGPGSTYLFSLKAGDRTQFKGPAG
                     NFYLREDPGRELLFVATGTGIAPLWSMLLANAERADPRPATLLWGLRSQRDLYYQKEL
                     AGLVRTNKTLNAITTLSRPEPGWSGESGRVLRLIEWQVSSVKNLAVYLCGNSAMIVDA
                     TALLQKKGLCPIYREKYYDDSGSPED"
     gene            58340..58771
                     /locus_tag="VLD60_08245"
     CDS             58340..58771
                     /locus_tag="VLD60_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB44986.1"
                     /translation="MKKQARKSVMVVTGSTAAGWRRAAEAMAAAFDCELHRVNLSGVV
                     SQYVGETEKQLNRIFEKAEESGAILLFEEADALFCRRSKVKDAHDRYANVETAALLQR
                     IEAYDGLAILATNRSAKIDPAWLRRAKVAKPKVSKKKATGR"
     gene            58847..59860
                     /locus_tag="VLD60_08250"
     CDS             58847..59860
                     /locus_tag="VLD60_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB44987.1"
                     /translation="MATRQLTPAGIMRLGLGFWGSKTLLSAIELGLFTELAKGPLTLQ
                     ALTTRLNLHPRSAQDFFDALVALGMLKRTGTRYANTSETAQFLDRNKPSYVGGMLEMS
                     NARLYRFWGSLTEGLRTGKPQNEAKTGEDFFGALYADPQQLEGFLKAMTGLSIGAGRA
                     IAKKFPWKKYRTFADVGCAQGGVAVEVALAHKHLTGFGMDLPVVQPVFEAYTQDKGVA
                     SRVKFHHGDFFKDLLPKVDVIIMGHILHDWNLDEKMMLLRKAYDALPPHGALIVHEAL
                     IDDARKQNAFGLLMSLNMLIETHGGFDFTGADCRKWMKEVGFRRTQVERLVGPDGMVV
                     GYK"
     gene            60365..61741
                     /locus_tag="VLD60_08255"
     CDS             60365..61741
                     /locus_tag="VLD60_08255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB44988.1"
                     /translation="MEPLALPASSKAIVMVSPSENCARWVNVDLRFVIATIVVFAAVA
                     TTTIRFSHEVFDPLADSLMIRGWAAVIARPSLLWFTMGMLLIVVRTLLWLRYHPFASV
                     SAEEAPRMSIIIPAYNEGAMVRRAVDACVRAEYPRDRLEIIVVNDGSTDDTGAHVDRA
                     RRDCPDLVQAVHLPVNGGKREALAAGFARATGEILVTVDSDSLIERGALLALAGPFHD
                     PRVGAVAGKVSVLNRFQSILPRMLHVRFILSFDFLRSVQSSYGTVYCCPGALSAYRTS
                     VIKPLIPTWLRQRFLGSDCTIGEDRALTNDVLASGYQAVYQRTAVVHTLVPESYQKLC
                     KMFLRWDRSYIREEIRLWKIMWKLPFPSFVLTMLETTITNLRYPVAYASLFLMVYMTM
                     QDPWTILRLLLSIGLVSLFYTLYFLHSERSREFLFGIVYAYFYFVSLLWIFPYALMTV
                     RNRRWLTR"
     gene            61993..63054
                     /locus_tag="VLD60_08260"
     CDS             61993..63054
                     /locus_tag="VLD60_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB44989.1"
                     /translation="MKFLKPDKDLWSDNLDRRQLLKALGVAGAVLALGGRQTLAEMVV
                     PVSEAGGAAAGAVPTRALGKTGIQVSALCFGGAHWGRTKDDAEAIRILHEAIEAGITF
                     LDNAWEYNGGRSEELMGKALQGRRQQVVLMTKLCSHGRDRKVALQQLDDSLRRLKTDY
                     LDLWQIHEVIYEDDPDRHFVPEGAADALLEAKRQGKVRFVGFTGHKHPSIHLKMLARD
                     FPFDTCQMPLNVFDGTYSSFEQQVLPVLHQRGIAPLGMKSLSGNAEAVKQGIVTVEEA
                     LRYVLSLPIASLVTGIDSRKVLQQNLDIVRRFRPMTVAEMETLRARVARYAKDGRFER
                     FKSTRDYDGRVGRDQHGLS"
     gene            complement(63165..64079)
                     /locus_tag="VLD60_08265"
     CDS             complement(63165..64079)
                     /locus_tag="VLD60_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HSB44990.1"
                     /translation="MPPSLHAFLVLFLLGASLRFYAMLTKMHAERLGSFVFSVSLPAT
                     ILVSLDRVSFAPTAWKLPLAACLVTLPVVLLSWPLVRLLRLSRPTQGSLLLSIGCINS
                     VYFAYPVTLATFGEEGLAQAILFDLGQTTLTLTVLYALAVWHGTASPSARSSLARLLS
                     APPLWALTITLALKAAGLHLPSWLHDALTPVHLTTMPLASLVLGLSISLDALRRTWQL
                     TCLGVSVRMAGGLLLGLMAAWLLDLTGMERAIVILIAGMPSAVTAVIFATETGLDEDL
                     VASIVALSICVGVALLPWLPRLASALLG"
     gene            complement(64529..64768)
                     /locus_tag="VLD60_08270"
     CDS             complement(64529..64768)
                     /locus_tag="VLD60_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44991.1"
                     /translation="MDPQTPVTDRILGTIKRTHGCDLDTLAERLPEFTWNQVFLEIDR
                     LSRRGEIMVTCGTGGRYMIYLSDHKKGPKTFQVRP"
     gene            complement(64828..65274)
                     /locus_tag="VLD60_08275"
     CDS             complement(64828..65274)
                     /locus_tag="VLD60_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44992.1"
                     /translation="MEVFSRRTVIPLLALILLSAVFFLLIPAHIHGTVTPSVLLVVAT
                     PTDRLAPPVEWVDDLEHRLMTYFTKKYPTYDFAPYADELDRIRGAVTLGDRWRARREM
                     GRFLSMLTSRAYGLGDDAAEELADLSQRLMPDEQFGIVYPRSGSDP"
     gene            65640..66605
                     /gene="pdhA"
                     /locus_tag="VLD60_08280"
     CDS             65640..66605
                     /gene="pdhA"
                     /locus_tag="VLD60_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HSB44993.1"
                     /translation="MDRAELLSLYRQMLLIRRFEEKSAEMYALAKIAGFLHLYIGEEA
                     IAVGAMAALRPNDYAISAYRDHGHCLARGADPGKVMAELFGKATGLCKGKGGSMHLVD
                     LAHRFMGGYAIVGGHIPLATGLAFAIKYQALDLVTACFFGEGAVPSGQAHEAFNLAAL
                     WKLPVIFVCENNRYGMGTPVERAIALYADVAETARSYGITAERVDGMDVLAVRSSMDK
                     AVKQVRAGRGPWFIEAMTYRYMGHSMSDPAHGHYRTKEEVEEHRKRDPLLLLKESMVN
                     QNLGTEADFKQLERDVGEVVAAAVKFAEDSPFPSPSDLYDDVLQE"
     gene            66614..67582
                     /locus_tag="VLD60_08285"
     CDS             66614..67582
                     /locus_tag="VLD60_08285"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HSB44994.1"
                     /translation="MLLSYREALNQAMREEMRRDQRIFLIGEEVAYYQGAFKVSKGFV
                     EEFGPQRVIDTPITEAGFTGLAIGAAMAGLHPIVELMTMNFGILALDQIVNNAAKIRY
                     MSGGQLSVPLVIRGPGSAAHQLGAQHSQSLEAWFCHVPGLKVIAPATPHDAKGLLRSA
                     IRDPNPVIFIEAQLLYGTKGEVQEGEYTIPIGVAEVKRVGRDVTIVAYSKMLFLALEA
                     ADQLSREGIEAEVIDPRTLKPLDLQTIVLSVKKTGRLVIVEEGWRFCGLGAQIAESVY
                     SAAFDHLDAPIMRVAGEEVPMPYCRPLEDAAIPDLPRVMEAVKSLL"
     gene            67596..68798
                     /locus_tag="VLD60_08290"
     CDS             67596..68798
                     /locus_tag="VLD60_08290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSB44995.1"
                     /translation="MASRVVMPKLTDTMEEGVLVAWKKREGDHVQSGEALAEIETDKA
                     IMDLEAFASGILRKILVNDGETVQSGTLLGVIAEADEDITSALTDTVTAAPSTGGGGK
                     PTAPAPAVTPPTSPTRTEGARPIASPRAKALAAERGIDLSTITGSGPGGRIVEEDLPK
                     IPAPVTAALPAGTDQPLTQMRKAIARATTQSKAPVPHFYLTREIDMEAAEQFREQFKQ
                     RRQPHPSITDLLIKAAAIALTRHPAINVSYVGEAIRHYAHIDIGVAVGMDDGLITPVI
                     RDCGAKTLEAISTESRALIERAKQKRLQPPEYTGATFSVSNLGMYGVDNFIAVLIPPQ
                     AASLAVGAIREVPVVAAGSVKIGRRMQVTLSCDHRAIDGVMGAEFLKELTRILEHPQE
                     LVIPVRTS"
     gene            69122..69436
                     /locus_tag="VLD60_08295"
     CDS             69122..69436
                     /locus_tag="VLD60_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB44996.1"
                     /translation="MAWGPDANTEDELEKLAAVRAYLHEQFPGASIRDSYDAGRIAEV
                     FRIETDGVEGLRDAVVSTEFLDEYPPEKMGKALTGLRVAEHLRSAKGKEVLVTSWGVE
                     EK"
     gene            69755..70996
                     /locus_tag="VLD60_08300"
     CDS             69755..70996
                     /locus_tag="VLD60_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HSB44997.1"
                     /translation="MTLSQPSARPRQAAVLFILITVMLDVLSFGIIIPVLPKLVENFM
                     SGDTAQAAAIYGFMGMAWALMQLLCSPIQGALSDRFGRRPVVLLSNFGLGLDYILMAL
                     APDITWLFAGRVISGIASSSFSTAGAYIADVTPPEERAAGFGMIGAAFGLGFVLGPAV
                     GGLLGASDPHLPFWGAAATSLVNACYGYFVLPESLPREKRMNFSWKRANAVGSLVLLR
                     SHHELFGLSAVAFLNYLAHAVLPSTAVLYVGYRYGWGSTAVGFMLAAAGINAMIVQGL
                     LMKPLTARFGERKTLLAGLCCGAIGFCVYGVATDGWIYCAGIPIMAFWGLAGPSAQGL
                     MTNRVSASEQGQLQGAIAGLSGIAGLIGPGLFTQTFALFIGPRAGWHLPGAPFLLASF
                     LLLLGMALAWRATRGGHEKTG"
     gene            complement(71060..71950)
                     /locus_tag="VLD60_08305"
     CDS             complement(71060..71950)
                     /locus_tag="VLD60_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB44998.1"
                     /translation="MSSRPNLRSSRRRPSRRRRRTLKTFWRYLRARWKTSRRILKAVL
                     ASPPSVRAVVILSAILLFWLGVNWTYHAYYKPSEILFPLDRTLNKSPAETWKEYGLLF
                     REHATAVITPELLAALAQVEGAGNPVARTYWRWQPSWNPLEWYRPASSAVGMYQITDG
                     TFQEAKRYCIHDHVVVEDGPWHDMQSCWFNNLYQRVLPSHAIELTSALLDRQVVSAVG
                     QRRSGTVTLRNKQDLAAVIHLCGAGAGQAYAARRFRLSPHQRCGDHDVRAYLIRVNAM
                     KQQFAQLASGGKQPRLVSPR"
     gene            73271..73954
                     /locus_tag="VLD60_08310"
     CDS             73271..73954
                     /locus_tag="VLD60_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB44999.1"
                     /translation="MPQNRCAMIVVVGLLVLTASWSAAQQPASQAESSLEPEFRQLRS
                     KLSAMPEFSGTDAESKFRLAEELAHRGDVQGAVETYRAAIHLKPDWPDPYRGLGQVLL
                     DHHDYAEAIQALQTSIRLGRDDHHAFYWLGRAYMGKHDLAAAAVALERATELKPDDAE
                     TFADLGLVRMTQGDDVAAEQALKRSIQLKPDYAEAHQLREQLAKAKGNRETVKEAGMA
                     RLRSVFDRE"
     gene            complement(73956..74525)
                     /locus_tag="VLD60_08315"
     CDS             complement(73956..74525)
                     /locus_tag="VLD60_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45000.1"
                     /translation="MPSFHFRTATLTLVTTYLALVLSFWPSPLLAADVPAPPSTQIFR
                     ASDWKGGTDVAFEGCKANSGEYTLWVYAKGDRCGVPRENADRVVMFRQPLPGVSPIRT
                     TSQLPAGAYAAWAYGSGDPGHPWINLCAKTCVKGELPSTPGWVSLGAIEVREMHQLLL
                     KTWEQPDGHQLAIHAIVLSTSNAQPDWRP"
     gene            75191..76489
                     /locus_tag="VLD60_08320"
     CDS             75191..76489
                     /locus_tag="VLD60_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB45001.1"
                     /translation="MKLRISVWVLCLLVVIGGCQILPGHWRPGWNQRLPAGAPVPDTA
                     GSWKLTELEQKDGRFVALAISGGGSRAANFGAAVMLELQQRGLLEQVDVISGVSGGTL
                     PAVYYGLGDKAGAFTEPAVRQALGYDFQSSWIRRWFLPQNIFRYWLSDFTRSDIMVQV
                     FNNRLYHKATFAELRLHPKILLNSTVHNDHTRFTFTDERFAALHSVLARYHVANAVNA
                     SSAFPGAFQDVTLQGYVEPPLYLHLYDGGPIDNLGVQAILEYVNRNILGTSLDRLFPN
                     GCLIIVVDATPASEHPELSTHESSRKFIDYFVDDNALDAMDAMLMESRRTLLARMGIS
                     VEKQDQDMRGRLPVNDPGQCGCEVRHVALRHLMYAGNSGDTGLAERVTQIKTKFWVSE
                     EEQNDLFQSAKIIMKLLDDQKLLSDPSFKVPCTVVPPSKQ"
     gene            complement(76553..76957)
                     /locus_tag="VLD60_08325"
     CDS             complement(76553..76957)
                     /locus_tag="VLD60_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45002.1"
                     /translation="MGRRLLIIDDNPMICEALKDRLEAMGYTVIVAHDGRTGLALMAL
                     EAKQSPIRGVLLDLQMPIMDGIEVLRELRVQHPHVPIVMMTAEQDRVIREEALSLGAR
                     QYILKPVDASQLRQICEQNFPLTDHNGVDFLE"
     gene            complement(76929..77213)
                     /locus_tag="VLD60_08330"
     CDS             complement(76929..77213)
                     /locus_tag="VLD60_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45003.1"
                     /translation="MPSEARPGNHHVEYAQRESAIMDLLRASGKLTVEEIVQQMPDLS
                     WSQLFLAMDVLSRTGEIILWREGFTYTLEAANPAPVLETASYGTTASDHR"
     gene            complement(77261..77509)
                     /locus_tag="VLD60_08335"
     CDS             complement(77261..77509)
                     /locus_tag="VLD60_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45004.1"
                     /translation="MVLNEQPASMPPTESEPAVRPAHCPRCQGFLIPVRYGEWGRITG
                     TQTAHAWSCVQCGGIIDPVILANRRSYRTQTVSAHVDR"
     gene            complement(77513..77941)
                     /locus_tag="VLD60_08340"
     CDS             complement(77513..77941)
                     /locus_tag="VLD60_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSB45005.1"
                     /translation="MSQSNPSNNRFPVVFVLLCSCMLLTGSAVAAFASPDPVEIAFET
                     TSPYYHPQVAVVSAGTPVRWNNATGSPHSVRHDGCLTDDHCAFQSIAVPPDSSFLIAP
                     LPPGRYGYHCELHPVMRGTLVVLVPLARGDRMISAPEPTR"
     gene            complement(78123..78833)
                     /locus_tag="VLD60_08345"
     CDS             complement(78123..78833)
                     /locus_tag="VLD60_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45006.1"
                     /translation="MTQGARILVVDDEPQIRRSLQVNLEQSGYAVETVETGEGALRSF
                     LNRRPDVVVLDLIMPGIDGVEVVRRIRESSTVPIIVLSAMGEEARKVEALELGADDYM
                     TKPFGMDELLARIRSLLRRAAGAHSSQPVFIAGGLCVNFDRREVTLDQKPVKLTPTEY
                     DLLKYMIEHAGKVLTHRMLLQEVWGQAYVDQAQYLRVFIGQLRKKLEKDPTRPRFILT
                     DSGVGYRFSLEGNAPSAA"
     gene            complement(78830..80257)
                     /locus_tag="VLD60_08350"
     CDS             complement(78830..80257)
                     /locus_tag="VLD60_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45007.1"
                     /translation="MAAAASPPAVKSHDVNWTVVGIIGLLIITALAIVPLTLTIIHSR
                     ESDALVIDLAGRQRMLLERHIKEVLLASQGVDAQYKVTRAALKERVQALIHGGTTVVQ
                     VGRSGTAVVPAAPTDEIRVKLLEQWTLMEALFTNADAFLQTAQTGTQNGVARDALLRD
                     HAALLTIANDAVTLLTQHSEQNVQTLIGWEVVVVLMVVAVATLGTWRFLRAEQALKQS
                     QAMTVQALHQRDAIKSSLLSSVSHELRTPLTTIKTMLFSLRRNGASLPPAVRHEFLTG
                     IERELDYLNGLVGNLLDMSRLEAGTLTPHLEWQIVDELVEGAIRRVEPLLAGRPLYVL
                     VDPDLPPVSVDGVQIQQVLVNLLDNAIKFSPPESPIRLTAALAENFLEVRISNTGEGV
                     PPGELDRIFERFYRVQSGGSSGSPGTGLGLAICKGIVEAHGGSIFAESVPGHETTILF
                     QIPLAASLLASDRSASEPATVQGAS"
     gene            complement(80387..81751)
                     /locus_tag="VLD60_08355"
     CDS             complement(80387..81751)
                     /locus_tag="VLD60_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45008.1"
                     /translation="MLISRRALTVFIPCFLWLSVPTANVFATATIYKYTDDSGTQNFT
                     TDLDSIPEKYQQRVVPLESQSSSTSPPLESPAEQRAPSANVRVVTAHGEYRMGDHDTR
                     ADAVRLAVEAAKRDALEQVATYLESVTEVKDMDVTRDDIRTYTAGIVVVLDQTITTWL
                     EEQTVVIRADLTAQVDPDEVVQAIAALRENESAKNELVALRAETDQLHQQLDAANQAL
                     AAAVSPEQVQALSQERQDLLNQLQTNALVSQAWTDWVYVTPVVYPYPWIGVQQVRGLL
                     LQAQHLYPRNRHLPVLQQVVTTQAGTMPPSSPGVSVQASPHSSLLVSPPASGRVTAPP
                     PQTLLPGSANAATSANALRTPAPAPPQTLLPGSANAGTGANALRTPAPPQQFSRPLPP
                     TLQQVHPPHVAQPNPPVLRAPFSVPRQSPSTSPQHFGGRGGGHSGGGGRGGGGRGGGG
                     HGGR"
     gene            82203..83408
                     /locus_tag="VLD60_08360"
     CDS             82203..83408
                     /locus_tag="VLD60_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HSB45009.1"
                     /translation="MSFTKVTIMPSIPEPLKHRLVQLRRVLHQYPELSGQETNTAAVI
                     CKVLDGLAIKYRAHVAGHGVVADIPGRAGVPCVVLRADTDALPIQEDTGLEFASVHDG
                     IMHACGHDGHTAMLLGAAALLSEEKDLPAPVRLIFQPAEEKGTGALAMIKAGALEAAG
                     MIFGGHLDRHYHPGAIVVAEGAVNASSDNFTIEIIGQGAHGARPHESIDAVVVGSLMI
                     MALQTIVSREIDPARPSVVSVGQFHAGTAPNVIAGQARLEGTVRAQDPAVRQQLIASI
                     RRIAESIAQLHGAKIQVTVKEGTPPLINTPEMASLARRAAVTAVGEGNVLPLKTANMG
                     AEDFSYYMEKIPGAYVRFGSQVPGKEGFPAHSSKFDFDEEALAVGAVYYHAIAKIAGQ
                     RLSEHKSRT"
     gene            83428..85080
                     /locus_tag="VLD60_08365"
     CDS             83428..85080
                     /locus_tag="VLD60_08365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB45010.1"
                     /translation="MIRIREAREEDVGQIREIFLAVYGADYPHHEVYDELWLKRSVFT
                     DDALILVAEETDTGRVIGTASVLFDFGAHSDLVGEFGRLAVHPDYRRLHVGRLLMDKR
                     LEAIQNRLHVGLVVARTVHPYAQRISLAQGFIAAGFLPLKHVFHHRESFALLARYFGD
                     ALALRRNNPRIIPEAYALANLVMSQPPLTPDFIVDEDSAPYPTGGDYTIEQLQAEGYP
                     ALLRIERGRVRNREIFGPMRLDYGFFKLQARRTSYFLARSGGQIVGAVGYTMDPVEHT
                     VRVFELIALADDVVRFLLAELELKCRQEMGIEYIEIDVSAYAPRMQRTLLELNFLPVA
                     YVPAMVFYQVERLDIVKMVRLNKLQDLGPLALTEPVQAVADLVMRGFSSCVIAPRMAQ
                     AIKEIPLFQGMNSEQAIRLAGACTVREVRAGERLFAEHDPGDRLYILLQGQVAITCGS
                     PPVRIGTVRTGETCGEVSLLSARPHSATATAEGHVEVAELLQRDLADLIRRRPDIGVI
                     IYRNLAVGLGDKLLRSGNSRRDQGLAESEVLHLTSEPASARK"
     gene            85401..86006
                     /locus_tag="VLD60_08370"
     CDS             85401..86006
                     /locus_tag="VLD60_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSB45011.1"
                     /translation="MTSILLGGLLTVPYAMTALAGNEFTQASVGKGVLLVASPTLDDP
                     NFRKSVVLVLEHGSEGTLGVIMNCSTNVLLSEASPDLAVLKGTSYRLFTGGPVEPTRL
                     ILLFRLKEPPADARSVFDGVYVGGTPAVLERIITQAKPNETFRAFAGVAGWAPGQLQY
                     ERLQEAWAILPPDSIGIFDKDPATLWEDCPRRLQAPRVISN"
     gene            86065..>86637
                     /locus_tag="VLD60_08375"
     CDS             86065..>86637
                     /locus_tag="VLD60_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45012.1"
                     /translation="MDLQSEKSWRLDLGARPIGSSVVQFRVWAPRAKTVSVHVCHPSR
                     NPIPMESREYGYFEVTIEDIRAGDRYQYILDDQKIRPDPASRFQPDGVHQASAVVDPG
                     AFAWTDREWKGLPLKDLIIYELHTGTFTATGTFEAIIPHLDYLKHAVGVTAIELMPVA
                     QFPGRRNWGYDGTYLYAPHSTYGGPQGLKTL"
BASE COUNT        18433 a        23620 c        26624 g        17960 t
ORIGIN      
        1 aagaaaatga gcaaaagcgt tggcaactat atcgcgcttg aagacaagcc cggtgagctg
       61 ttcggcaaag tcatgtccat cagtgacgcg ctcatgttcc gttattatga attgctgacc
      121 actgaagatc tggctaaggt caaggttctt catcccatgg aggcgaaaca ggccttggcc
      181 cagttgctag tcactcgtta tcacggcgaa ccagccggga aagaagctcg aggaggcttt
      241 caacagaaat ttcaagagcg cgaatttccg agcgagccgg acgttcggtt gatcctgaca
      301 agcgccgacc tgaaagaggg aaaagggatc agcctcgccg atcttgtggc caagacgggg
      361 ctggttccca gcaagagcga agcgcggaga ctgatcatcc aaggtggtct ggaagtgaac
      421 gaccagaaac agaccgatgc caatgctgtc ttaaacctcg tagccggcca agcctatcgc
      481 ctgaagatag gccggagaaa gtttgcactc gtcgaactgc aaggctaagg ggcggcttcc
      541 gtcaaacaaa atgcgtacgg cccgagggtt gtctctgtga atccattaaa agtatgcccg
      601 tacgccgacg agaatgttaa gcgtcgaagc atcaaaatcg gcctcccttc cattgagctc
      661 gtacgttcca ctatctctgt tccaggcgag ctctgtgttg aatgcccatc gcgacgtgat
      721 cgggtagtca aacccggtgg caagccgtaa agcgaaggtg ctcgagaatg agtcggcatt
      781 gttggccgtc tggttgtcat tactgaacga atgtacattg accccggtac ccaaagagac
      841 gtatgggata aacgaaccca atccaagatt ttctctcgtc tcacgggttg gacggaattc
      901 gacagtcggc aggatggaga aaacgctcaa ggtgcccaat tggggtcccc agaggtcgat
      961 gctgtgctga tgccactcaa gcatcatgcc aacccgaatc cagtcattga gggtgtatag
     1021 ggcttcagca tttacgagag gacctacgct ggtaccattc ccaacattct ccaatacctt
     1081 cgaggtaggt atcgccgctc ctgcgcgcaa gcccaagacg ataccacgct gtccgggttc
     1141 gggtttcacg actatgactc cttgggcggc taattcctct ttgcttaacg tatcttgagc
     1201 atagaccttg ttacaaacaa cagaagctga catgtagaag attagtatca tgaggcaggt
     1261 tagacggagc caagatttgg taaagattgc aatgtacctg ctaaaggttc cagcggggaa
     1321 tgtgcaatta ttcaaaacag tgccacctgc tctgataagg tttttggcca tgatctgcgc
     1381 ctttgatctg gttaatggct caagtgaggg ataacttgta tctttctcta tagtgccatg
     1441 tcaataatgt aaaggttgcg acagtttgtg gtccgagcat cggagcgagt cctcgctaga
     1501 cacccgatgg gcgattcctg tataaaatgt gcctggtctg atgagacctt agatgtgttc
     1561 cgcttatgtt ctcgatcgct aaagccatga caagaattct gtagagactc gcgcacttca
     1621 ggagcatctg gcggtttggt gctgcatgtt taacgcagca tagcgaaaat acagcgagcg
     1681 ggcgtagctc agtggtagag tcctagcttc ccaagctagt tgtcgtgggt tcaaatccca
     1741 tcgcccgctc caaatcctcg tagcgatcat gtgggatttg aagcccacga caggggattc
     1801 cgaaaggggg tgagcccctt tcgtggggag cgcacgaggg ggttgacccc ctcagtggga
     1861 gccgggaggc aggcttcagg gccgacccgg cgaacggagt gtggttcaaa tcccatcgcc
     1921 cgctccaaaa agcttgctcg aaccggtgcc tctccgatgg ctcttggtgg ccggataaac
     1981 acttccagtg acccttcatc ctgacctccg tgccttgcaa taccgcattc aggtccaagc
     2041 tacctcgttg aagctcgcat gctgcttgct gcttcttatg ctgagtggtt gtcattatta
     2101 cgaagagtac ggggtactga agtccatcgc tgatattcag gatgaaaagg cggaattgct
     2161 caaggcgtat cggctatgtt tgagcaaata acaggacgat ctgccaaaag cgaaagaaat
     2221 ctgcggaccc tatacgcata cattgcgtga gatcgaggtc cggaaccctt cgaatcgata
     2281 agtggggact tatcagaaag gacgttccat gaccaggttg caatttgtcc tcgtgctggt
     2341 gacaacgttt gttggcagtg tagtcggtgg cgttttgagt gagcgcatcc tctcggtcgg
     2401 ttccgttcac gctcaaaaag caaatggagt gaatgcggaa gagtttctct tgatcgatca
     2461 gaatgggaag gcccgggctg ggctcggtct ggatccggcc ggtgaagtgg gattggtcat
     2521 gaccagtcgc gacgggtcga gaaagctggc attgtctccg gacggaccgg ttgtgctcaa
     2581 gatcagcgat aggagcggtc gtgtcttgtg gtcagcccag taaactggcc ggtagatggg
     2641 aatgactaga cggcttttgt gactttaccg cttcgcagac aacgcgtaca gactcggata
     2701 cgtttcgtcg atccatcaac gacagcgcga acgcgctgga ggttgggatt gaaagtacgc
     2761 ttggtcttgt tgtttgcgtg gctgacgtta tgaccgctct ggtgtttctt ggagcaaata
     2821 tcgcatacaa atgccacggc aagactcctt cactcgatta gagtactaag ctaacctggg
     2881 cgctcaatct aacatactgt ggccggatgt gacaagcgcc cgctagaaga gcgtgtctgt
     2941 gcgttaccgg tgtagggggg ctcatgactg atctcgatcg gctcgggtac gtggcagggg
     3001 gctgaccacc tgtgccttct ggccgcaact ccggaagccc tggacaacga agtctactgg
     3061 agatgtatcg ctcttggcat gctggccatt ttctcgccgg gtgccggact ctggtttctc
     3121 tacggagtga cgcttgggga ttggcccatt atcgtaacga acgccgtgac attcctcctg
     3181 acccgcgcga ttctccccct tcaaactacg cttcgaatcc tcacctgttc gtctatagca
     3241 agagtcgttg atcagctgag gtgccgaaag gttaacctat tgacgggttg atcgaggact
     3301 gcgaaaggac tgtcccatct ctttcggggc agtctgtgtt gcgacaacga ttgccggtac
     3361 gctgatgtcg aaggtcggat ctcctccatc gtcgaggcgg tctttgccga cgctgtagag
     3421 tttttgttgc gtagggctcc acaacatcgg cagtccgcta aagggatcaa agtattgcga
     3481 tcccacttga gcaagtctcg tgggcacagc agtttgtgca ctcgggcgtc tgagctgaat
     3541 ttgcagcgag gccaaacgca ggcgcgtatc cgtttccatg agtttgcggt gaaaggtttc
     3601 ccagtctggc gcgacaggat atggattcaa caggttctcg atcatgcctc gttgtagagt
     3661 tcctgtcact tcgtgcatag caggtaggcg tttcgcgccg ttttccgacg cactgatgag
     3721 cgcgtcataa taggcggcat aggtctccca tgcctgcgcg gattgaaggg tgaggccgaa
     3781 ccatgtgcga gcgggcggat gctcgatacg gtcgaacgcc tggagcggaa gattggccga
     3841 ggttaagagc cattcttcgt cggctttggc ctgttcgctt cggctatctt tcatgattct
     3901 accggcacgg ccttctttga cagccagggc gagctgattc cggatcggcc agcgcaggga
     3961 atgttctgat ccagagaggg gaagcgtcaa ttgcaaaccc atcgtcaaga acgctttgtc
     4021 cacggtgggc ttggccagga tacgtgagag gaggctgagg tcgtcgctga tcagaatctg
     4081 tgagaacatc tttgtatcga ttgtcttggc ttctcgaagc accactcgcc agaattggaa
     4141 ttccctattc agccgatcaa gcccttcggt gatgccgaga gagaatccct cactaatgta
     4201 cagccggtgt acggccatga ttgcttggta gcggggagtg actcgatggc tgaatcccca
     4261 atcttcgaac ggcatcccga gccaacgctc atagcgtgcg atgagaatct ggtgttcgcc
     4321 cgtggctgca cgaagcgagg cctgctgttt tcgaaattcg gtcagcggct catcggattc
     4381 ccaggacggc gatgagaact caggtgagat ggtgaagctc agttcggatc ggctcggccg
     4441 attctccacg ttgccggcat ggtgtgagcc actgaactcc tccacccaaa tctcgtagcc
     4501 tgctttggcc ggatcaagcg gggctgcggc ggtcagtccg aataaataga aatagccgtt
     4561 gtgataggga ttattgatga acagttgtgg cccttcggag agcctcgcga atcccccgct
     4621 gggaagcgcg cccttagctg accagaacat tccggtgact tggaacaatg cccagaacac
     4681 agctgccgca ataatcaggt gtttcatggg tgcatacgta aaagccgctg catcgcgtca
     4741 tctttccgaa ctgtgtatat gaccagtcct gtgttcgtcc actagggaaa ttgagcaatc
     4801 cctatgccga aggcgggcag gaaaagtcgc gctgtttcat gacgttattc tgatccgagc
     4861 tggtgagatc gtaggaggtg aaggattcct gcacattaat gaacttgaca aaaattgctc
     4921 ctgtctccta gagtgcctgc cacattacgt ttcctgggta agggaagagg gtgcaagtcc
     4981 ctcgcggtcc cgccgctgta aatggggacg aaacccgtga taaccactgt ctgctccggc
     5041 ggatgggaag gcacggggag taggacgaac catgagccag aagacctgcc tggagaattg
     5101 accatcgttc cctcgagggc tggggaacag gcgaggatga agaagcgggt gcaatcctca
     5161 aggtctgtcc caggccttga ggatttttta tttgcgcggt tctgcacgac agcatcggtg
     5221 gtgggttggg gtgttgatcg gtgcaggtgt gctcgtcgtt gcatgcacat ccttcgcgag
     5281 tacagagcat gaaagttcgg gcatgaaacg tcggcagcag ggcattctca ccggcatgcc
     5341 gtttatggcg aacgtctcag cacgcgccta cgtcgatgat gccggccgac gcctctatct
     5401 cgccaaacct cccgcgagga tcgtctcgtt ggcgcccagc atcaccgaga tgttgtttgc
     5461 catggaggcg ggggatcaga tcgtcggcgt tacggacttt tgcgactatc cgcccgaggc
     5521 tctgaagaag ccgaaagtgg ggtactccaa tccgaacttg gaatcgttgg tggcactcca
     5581 gcctgatttg gtggtggcgc cgaatgattt tctgaagcct gatgtcatcg tcaggctcga
     5641 gcagctgaag attcccgtgt ttattctggc tgacaagaac gttgacgata tttttgttca
     5701 tatccagaca cttggtcgta ttgtggggcg atctccgaaa gccgatgcgg tggcgatgga
     5761 ccttcggcgg caggttgctg cgattaagca gcgtgttcaa cgtatggtcc ccgtacgggt
     5821 gctctacgtc ctgaacagtc agccattgat tacggtcggg ccgggaagtt ttatcgatca
     5881 actgatcgga atggccggag gtatgaacgt ggctgcaaag agcgctaccc cctatccgcg
     5941 cttcagtttg gaagccgtcc tccaagaaga tcccgaagtg ctggtgtttc ctgtcggcaa
     6001 ggcggaaggc attccggaga gtgagcagca gacctggctg cagtggtcga caatgactgc
     6061 ggtcaagcgg ggccggcttc accagatccc tgccgattgg ctcaaccggc ctggaccgcg
     6121 aatcgccaag ggacttgaat cgcttgcggc aattttacat ccggacagcg cgatagacag
     6181 tcgagtccac cggtaacgcg agtcacgtat ggtagatctt gtttcatcat gggcgaatgc
     6241 cggctcagtg ctgactgtat ctcgttgggt gacggtgatc gggggactgc tgctggcgag
     6301 tctggccgta ctggttctct gcctgcagtt cggtgccacg tcggtcgacg tgaggacagt
     6361 cgcaacggcg atcacgcaca tgtttcgtgg agatgagagt ggctccccaa gcttaaggac
     6421 atccgagatc attatggtcc agattcgttt cccgcgggtg ctcatgggtt tcctcgtggg
     6481 agccagtttg gcggcggtcg gtgtgacgct tcaagccctg ctgagaaatc ccttggccga
     6541 tccgtatgtg ctgggggtct cgagcggctc cgctttaggt gtctcgttgg cgatcctctt
     6601 tggaatcggt tccgccgtca tgctgttgcc gttgctgccg atctttggat ttgccggagg
     6661 gctcctcgca ctcctgttga tctatcggct ggcccagtcg aaaggccgac tgccaatcca
     6721 tagtttgtta ctggcgggcg taattctcaa tgcgattttg acagccttca tcatgttcat
     6781 tacctccatc atggagccaa atcgctcggc gggattgatg gcgtggctga tgggatcgct
     6841 cactgctcct gggtatccgg cgttgggtgt cttttccatg tatgtggtgc ttgtcctcgc
     6901 gatcctcatg cacaaggcac aactcttaaa tgtgctcact ctgggtgaag aaacggcccg
     6961 ctcgttgggc gtggagaccg aaggggtgaa aaagcagctg tttgccctga cggcattact
     7021 gaccggcgca attgtgtcgg tgagcgggat gatcgggttc gtggggatgg tcgttccgca
     7081 tgcggtgcgg ttgctcatcg gatcggacca tcgattgctg ctgccggcat cagccctagt
     7141 tggtggcatg tttctcgccg tggccgatac gttggcccgg accatcatgg caccggcaga
     7201 aataccagtc gggatcgtca cagcgctcgc tggtgggcca ttctttctct atctcctgct
     7261 ctgccgaaaa gatcggatgc tatgagatga cagccgctcg gaccggaaca gcgcaatcct
     7321 cgctggcggt gaatcctcct gccattgaag ttgagggact atcgtttcgt taccacacga
     7381 gggaagaaca agggagaaag tggacgctgg atcatctttc attccatgtc gatgccggag
     7441 aaattcttgg tattgtcggg ccgaacgggt ccggcaagtc gtcactattg aaagtgtgca
     7501 cgggcctctt gccggctggc gaaggacagg ttcggctggg aggcgttccg cttaggaagc
     7561 agagtcaatc cgacatcgcg cggatggtgg cgctggtacc acaagaatac gttcaagtgt
     7621 ttccattcac cgtggccgag actgtgttga tggggcggtt tccccatcgc actgctcgtt
     7681 ggtggagcct gggaatcggg gatgagacgg caaacgatct cgcctgtgca caccaagcca
     7741 tggtcgagac cgatgtgctg ccgctcgccg atcggttggt atcagaccta tccgggggag
     7801 agcggcaacg agtcatgatc gccagagctt tggcccaaga accaaatatt cttctcctcg
     7861 acgaaccgac cgtgtttctc gatatcaatc atcaaattga gatctgttcg cttatcgccc
     7921 agttgagagc tgaacgccga ttaacggtgg tattagtgtc acacgatttg aatgtcgcca
     7981 gtcaatattg cgatcgcgtg ttgatgctca aggaaggccg gctctgccgc atcggaccgc
     8041 cggatgagac gatttgtccg gacatccttc ggatggtcta cggatgtgat gtagtcgtcg
     8101 atgcccaccc ccagacgggg aggccgcgcg tgacgatgcc aatcagcgtg actcgattct
     8161 agaaggcagc caggggcctg tccccggcca ggctgtgctg tacaaggggg caaacagttt
     8221 ttcaggaaag ggccgattga agaaacgatg cgggtgacgg ggaagatggt gcaattccat
     8281 cacggtgccg ccactgtaag cggggagtaa ctctgcatca tgccactgtg tgcataacca
     8341 cacgggaagg cgcagagacg cgacgatccg caagtcagga gacccatccc gtaattgctt
     8401 cgactcaacc cttcgagcac aagggagatt cgtcatgacg cggtgttctc gtttctgtcg
     8461 agtgtggtgg tgtttacttc ccctgtttgc attgagctat cccgtccatg ctgctcaaga
     8521 gatcgaagtg gcgtccgctg atcagacgga catcatcgag acccgcgagg tcgtggtgag
     8581 tgcgacgaaa actccggtac ctgtcgccca gctgaccagc gcggttgagg tcattacagg
     8641 ggaagaacta gagagaaaga agatcaagac agttatcgat gcgttacgtc ttgcagaggg
     8701 cgtgtttgcc acgtcaagcg gggggcccgg tacggaagct tctgtaaaaa tgcgcggagc
     8761 tgctgccaag cataccatgg tgctgatcga tggtgtgatc gtgaacagcc cgacgaccgg
     8821 agtctatgac tttgcgaatc taacgacgga aaatatcgat cgtatcgaaa tcgttcgcgg
     8881 agcccagagt atgttgtacg ggtcagatgc catgggcgga gtcattaata tctttaccaa
     8941 gagaggagtc gacaggttca cgtcaagtgc gttcgttgag tatggttcct ttgcctcgat
     9001 acgtgaaggt gtccaggcat ccggtgccaa ggggcccttc gacttttctg cctcattatc
     9061 tcgctgggac accagcagct tttccgcgat caattatcga cgcggagcgt acgagcgaga
     9121 cggattccat aactggcaag cctcagggaa gctcggcatc tccttgccga aggacgggcg
     9181 cttggagttc aatcttcgct ggtataattc tgatctcaac ttcgatgggt ttgcagacag
     9241 cggtgcgccg gcagatgtgt tcggggcaaa acagacaaat cggaatctga ttatcagcgg
     9301 tacatacgac cagccgatta catcctggtg gacccaaaaa ctcacgctcg cacaggcaaa
     9361 tgaacaatta ctcagccaaa gtggaaccgc cggatacaat gtcaacacag ggcagaacat
     9421 aaccgccaac cccaattcct gcttccccaa tttcgatacg tgcttcactc cattttcgac
     9481 caatctagac acgctcaacc agcggctgga atggcagcat aattttcaag tgaggaagga
     9541 actccttctc agtgccggct atcaatttcg tcaggaacaa ggtgatgcgt cgggattttt
     9601 tggatcggcg tcgcccaaga agctgatctc gtcgaatgcc gggtttgctc aggctcaagt
     9661 caatctgttc gatcgcgtgc tgtttacggg aggggttcgc caggacagtt ataatgtctt
     9721 tggtgatgcg accacctatc gtctgactgg tggctatttg cataaagaaa ccggcacgaa
     9781 gattcgtgcg agctacggga cggggttcaa ggcacccacg ctgaacgatc tcttctttca
     9841 agggttcggg aatccgaatc tccagcctga aaagagcaag agtgcggatg tggcagtcga
     9901 tcagagcctg ctccaggata agctgaatct gagtgttggg tatttctgga atcactttaa
     9961 caatttaata acatttctgt cggtaccgag tcccaattgc ccagcctctt cttttggctt
    10021 ctgtcccatt aatattgcat cggcaaggac ggaaggatgg gagatgagtt tcaaatttga
    10081 gctcatgaaa gggcttgatt tccgggggca atacactatg acggtgacac gagcatacgg
    10141 tgtgccaggt cgcccggaaa acaccaggct tccacgatgg ccctacgatc aggcgactct
    10201 tggcgtcagt tatcagccga tcgatgttgc ccgaatcaac gtggattatc gattcgtggg
    10261 ggcgcgcaac aacaatgcgg ataacacgcc gagccaacgg caagggtcct ttggtgtcgt
    10321 caatgcttca gcgacctacg atgtcacgaa gaactggcag gtcttcggcc gcgtcgataa
    10381 tctcttgaac caaaaatatg aggagatcct ctatttcggc acgccgattc ggtcggtgta
    10441 tggtggtgtg aagtttacgt attgaatggt gcaggttgaa gatgagattc ccccgtttgg
    10501 tcattgcggg tacgcacagc ggcgttggca agacgaccgt gacgttggcg gttttggcgg
    10561 ctttgaaaca tcggggccgc caggtgcagc cattcaagtc tgggccggac tttatcgatc
    10621 ccggtcacca cagtgcggca accggtcgtc cgtctcgcaa tttagatggc tggatgctgg
    10681 gggaagctgt caaccgtgcg atcttcgttc gcgccgccgc cgatgcggac atctccgtta
    10741 tagaagggat gatgggacta ttcgacggta gttcaccggt gagcgaaatc ggcagcaccg
    10801 ctgagctggc aaaacagttg gcggcacctg tcctgctcgt gattgatgga agcaccttgg
    10861 cccgttcagc ggctgcgatg gcgtcgggct atgcgaaatt tgattccaac ctgcgagtgg
    10921 tcggcgtgtt attcaatcgg atcgccagcg aggggcacta ccggctgttg aaggaagcgg
    10981 ttgaagctga aaccaaccta gccgtcgcgg ggtatctgag gccggatcca tccttgacgg
    11041 tttcggaccg gcatcttggt ttggtcactg ccatcgagca aggatcgatc gatctctacg
    11101 agcggcttgc caaagtggcg gcagaaacgc tcgatttgga tcgggtcgaa caaattgctc
    11161 gttcaacgag cgagctagac tttcttccta tctctgtcca tggaagaggc gggcaagggg
    11221 agggagtgcg tgtcggtgtg gcctacgatt cggccttttg tttttactat ccggagaatc
    11281 ttgagttact ggaagccgag gaagcggagc tggtgaagtt ctcgccattg aacgatcagg
    11341 tcttgccgga tgttgatcta ctgtatctcg gcggtggcta cccggaactt tttggcgaaa
    11401 agttggcagg gaacttggct atgaggaagg cgatccgttc gtttgccgag cgtggcggag
    11461 cgatctacgc agaatgcgga gggatgatgt atctcacgca agccatccgc gactttgccg
    11521 gcttatcgca tgagatggtc gggttgtttc ccgctgaagc tgttatgcgg cagccaggct
    11581 tgaccttagg ctatagaacg gtggaactca cgcgagactg tatcctcggt gcgtctggca
    11641 ctaccgcacg cgggcacgag tttcattact ctacgctggc tccgagagga tcgctggcct
    11701 atgcctgcgc actcagcgat gcgcgcgggg agtcaaaagg ccaggatggg ctcgcaaagg
    11761 gcaatgtgct cgcgctctac acccatctgc actttgccag tcagccgggg atcgcgaaag
    11821 cgttggtggc ctctgcacgt cagacggcgc gaagcgtgaa atgacggatc aagccgaaca
    11881 caaagccaag atggaacgct tgaaggcgtc ggtggatcga cgcattgagg cggctcaaca
    11941 agagaaaggc ctgttgattg tctatactgg agcaggcaag ggcaagacga ctgctgcgct
    12001 ggggatggca ctgcgctgcc ttggccatgg aatgaaagtg gcggttgtgc aattcatcaa
    12061 aggggcgatc gatacggccg agggacgggc gctgaattcg ttcggcgatc agattgcctt
    12121 tctgcgcatg ggcgaagggt atacctggga aacccaagat cgtgagcggg ataagaaggt
    12181 cgcccaacaa gcctggcaaa aagctgccga gtttctgcgc gatccctcct atgcaatggt
    12241 gattctggac gaattcaaca ttgccttgca gcatggctac gtcgagttcc cggatgtatt
    12301 gccggcgctg cgtgcccgcc caccaatgca acatgtcgtc attacgggac gcggtgccaa
    12361 taaggaattg accgaagagg ctgatctggt gaccgagatg aagcaggtca aacatccctt
    12421 ccgaaagggt atcaaggcgc aggccggcgt ggaattctag tcgaagccag cgcaagagga
    12481 gtgtacatga gaggaccagt tcaaaagacc tgcgaagcct gtggggaaag ctttgagtgt
    12541 ggccagtacg gctgttggtg ttgcactgtt cacgtcaccg accaacagat ggattggatt
    12601 gccgctcgct ttcaggactg tctctgtcct gcttgtttga ataaggttac catcggtgcg
    12661 ctggagagtc tttctctcaa attaattcgt cccacgcgag cggagtgacg caccttcatg
    12721 aacactatcg tgaatccatg tctgaggagc ggacatgagt cggcaaactg acatcgtgtg
    12781 tggcaatgga gcattttccg atgacgaacg ggcggccgtt taccgcgtga tcttcgagcg
    12841 gcgggatgtc cgtcggaact ttatgcaaaa accgatcact gatgatgtgc tagcaaggat
    12901 tttgacggcc gcgcatcacg ccggatctgt ggggttcatg cagccgtggg attttgtggt
    12961 cattcgacaa cgcgccacga agcgtgccgt caaacagctt ttcatgaaaa cgaatgccga
    13021 ggcggccatg caatatgagg gcagccgcgc gaccctctat cgaagcttaa agcttgaagg
    13081 gatcgaggaa gcgccggtca atattagcgt gacctgcagc cgtcagcggg gcggtcggca
    13141 tgtgttgggt cgttccacag tgcgtgatac ggatttgtac agtacctgtt gtgccattca
    13201 aaatctttgg ctggcagcac gagcagaagg tatcggggtt ggatgggtca gcattctgga
    13261 ccatggagca ttgaagcagg tgctcggtat tccgaggtca gtcaaagtgc tggcctatct
    13321 ctgcctcggc tatgtcccgg agtttgctgc ccggcctgat ctggaacagg cgggttggcg
    13381 gagcaggatt ccacttgagc aactgattca ctctgagttg tggggaaaca ggatacaagg
    13441 tgtgaggagg aacggggatg cgcatcacca gagtctacac gagaacgggg gacgcgggaa
    13501 aaacaaagtt ggccggaggg caggaagtat ggaaggacag tcttcgagtt gaggcctacg
    13561 gcactctcga tgagctcaat gctgcggtcg gggttgcgcg ggtgatgaat gccgagctga
    13621 tggggagttt ggcggaggca gaacaactcg aagaagatct ccgatggatt cagaataaac
    13681 tatttgatct cggcgggatc cttgcgacag ttcctggcca agagttcaag aatatgcccc
    13741 aggtgacgcc gagggatgtg actcggttgg agaagctgat cgatcgctgt caaaaagatc
    13801 tgcaaccctt gaaggagttc atcctgccgg gcggtggaaa ggtgtccggc tttctccacc
    13861 aggccaggac gatttgtcgc agggccgagc ggctgtgcgt gcgtctgtcc cgtgaagaac
    13921 tggtcgatcc gacaatcatt aagtatgtga accgactgag cgatgcgctg ttcgtgttgg
    13981 cccgctgggc ggcgaagaca caggatgaac cggagttctt gtgggagcgg gatgtcaaaa
    14041 gccactcgaa gtaaaggcgg gatcaaacac aagaggactg gccggatcat attggttctt
    14101 ggcggtgcag cctccggcaa gagcgctatc gcgttgcgat tggctggtat gaagaaaccg
    14161 aaagcgttcc tcgccacagg acaagggctc gacgatgaga tggcgacgcg gatcgcccgc
    14221 catcaagcgt cgcgcggatc cgattggaaa acggccgaag tgccggctga attggcgacg
    14281 tggttcaagc aaaagggtac ggcctatcaa accatcgtct tggattgtgt caccctgtgg
    14341 ttgagcaatc ttcacgaagc aggggtatcc gcgagtgcag tggcgaggag agtcgacgaa
    14401 ctccttgcgg cgatgcggag aaccaacgtc ctgatcgtga tcgtcaccaa cgaactgggc
    14461 ttgggtgtag tgccaatgaa cagagccgcg agggggttcc gggatctggc cggccgtgtg
    14521 aatcagcaga ttgcggcagc agccgacgag gtccatctcg tggtggctgg catttcactt
    14581 cgcctgaaat aaacaaagga acaatacgat gtcgttgcac aatctcctca gccggatcca
    14641 gaccccggac ccacgcttgc tcgacaaggc gcagtcgcgt cttgatcgtc tgaccaagcc
    14701 gctcggcagc ctgggtcggc tcgaggaact tgccgcgcgc tatgtcatga tgaccggcga
    14761 attgaaaccg aataacccgc gcggggccgt ctttacgttc gcggccgatc atggtgtggc
    14821 tatggaaggc gtgagtgctt acccgcgtga agtcacgccg cagatggtgt tgaactttct
    14881 ccgtggaggt gccggcgtga atgtgctggc gcgccatgtt ggggtggatg tacgggtcgt
    14941 agatatcggt gtggcctacg actttggagc gatctcgggg ctgatacaga agaaaatcat
    15001 gcatgggacc aaaaatctct tggtcgagcc tgccatgacg cgagcgcaag cagagcagac
    15061 catcgaagtg ggtattgaac tagcgagcat tgcggcgcag gaaggaattg ggctgatcgg
    15121 cactggcgat atgggaatca gtaacacgac tccgagtgcc gcaatcacag cggtcatgac
    15181 cggtcggcca gtagctgaag tcacgggccg cgggacaggg attgatgatg cgggccatgt
    15241 gcgaaaggtg gcggtgattc agcaggcgtt ggatctccat cgccccgatc gcaccgatgc
    15301 gctggatgtc ttgtcgaaag tcggtggatt ggagattggt ggcctggcgg ggttgatcct
    15361 cggtgctgct gccgaacgac tgccagtagt gttggatggc tttatcgccg gagcagcagc
    15421 gttgatcgcc gtcggtcttc agccgcgttg ccgggaatac ctgatcgcct cgcatcgttc
    15481 cgtcgagcgc gggcatcagg caatcctcga ctatctcggg gtcaagccgc ttctcgatct
    15541 tgacctccgt ttgggtgaag ggacaggagc ctgtttgggc atgagtcttg tgtgtgctgc
    15601 gatcaaaatc tacagcgaga tggcgacatt cgacgaggcg ggcgtatctg agaaggagtg
    15661 attgagatgg gggcggtagc caggccgttt gtcttcgcat ggcattttct gactgccatt
    15721 ccgctgagcc gtgagcacca taccccgacg gctcccgagt tggcggcgtc gatggcctgg
    15781 tatccggtga tcgggctggt gatcggcgga gggctggctc tgattgatct ggctttagca
    15841 aggcttctct caccgactat cgtgaatgtg ctcttgattg tcctgctcgt gctggtgacg
    15901 cgcggactgc atcaggacgg gttggccgat acactcgatg gattggccgg cgggcggacc
    15961 ccggctgagc gtctaccgat catgcgcgat ccacgcgttg gagcaatcgg cgcaacagga
    16021 ttgtttctgt cgttgctcct gcggtatgcg ggactgatgg cattgcctca agcactgcgt
    16081 ctgccagcca tcatctgtat gcccgcattg ggacgttggg cgatggtgat gacggcctgg
    16141 tcgtctccct atgcgagagc ggagggaggc ctggcttcac cgttcctcgc gcatctgtcg
    16201 tggtttcatg tactggtgtc taccgcgatc attgcactgg cgctggtgct cggattcggt
    16261 ctgcttcatg ccgctgtggt tctggtgatc agtgtgatag tgctccttgt ggtctgggcc
    16321 ggttgtcgta tctggtttgg cgggatcacg ggggacacgc ttggcgcgac gaacgaagtg
    16381 gttgaaatcc tcttcctgct cctcctgccg cttcttttgt tgatgtcatg accggcggcg
    16441 atcttcttat tgcagcggca atcgatgtgg cggtcggtga tcctcgctgg ctgcctcatc
    16501 cggtccgtgg gatgggccta gcgatttcct gctgtgacga acagtttcac cggttctgca
    16561 agtctccgat gagtttgcgc atagcgggat tatgtctggc gatcggattg ccggttgtgg
    16621 tctatgggat cgggtggttc gcgatcgagc tggctggatc tgtcgcggcg ctgtttggac
    16681 aggcggtggg tatcgccctc gcatcgacga cgttggcctg gcgggatctt tgggatcatg
    16741 ccatggccgt gattcgtgaa ttggccgggg aaaatatacc agaagcccgt caggcgctgg
    16801 ggatgattgt gggacgcgat actgtatcgt tatcggagac tgagatagtc cgggcggcag
    16861 tggagaccat tgctgagagt acggcggatg gtgtgattgc accgcttgtg tatctgacgc
    16921 tcggaggtgc gccgctggct ttggcctata aagcgatcaa cacgttagat tcgatgatcg
    16981 gacatcggga cgagcgctat gtggattttg gctgggcttc cgctcgtctc gatgatcttg
    17041 ccaattggat tcctgcccga ctcactgcgg gctttatggt gatcaccgct ggattggcta
    17101 cggggcaatg ggagcggacg atcgagagct ggagaattct tctcagagat ggggccaagc
    17161 atccgagtcc gaacagcggg aggccggaag cagcgatggc gggtgcattg gacgttcaac
    17221 tcggcggaac aaattactat gacgggctcc tgcacgagca ccctgtcatg ggtgatggta
    17281 cgagaatgct cacgccaggc catatcaggt atgccttgcg gattatgacg atgacgactc
    17341 tgctgggtct cggcttggcg gtggtgtctt tgtggctcgc atgaaggcca atggtcatgg
    17401 tggcgatgtg tattttgcct cgcgtgaact gggtcgtgat gtcgaaaggc tgatcgattt
    17461 tagcgccagc atcaatccgc ttggaccctc atcgcaagtc tggaaggcgc tgtccaaagc
    17521 tcgtatagcg ttgcagcatt atccggaccc agcctgcagg gatcttcgtc aggttctggc
    17581 aatgcggtgg cactgttcgc ctgaggagat tgtcgtcggc aacggatcga cagagttgat
    17641 tcatgtgctg ccatcggcac ttgatattcg gaacctcctg atcgtcggtc cgactttttc
    17701 cgaatatgcc tcggccatgt cgctggtggg tggacgggtc acgacgatat gtgccgagag
    17761 acaccagggc tatcgcccgc cgcttgaggg agtcgtggct ctgctaagcc ggagaaagcg
    17821 tacatatcgt gatattggag ctgtcctctt gtgtaatccc aatagcccga ccggacaggc
    17881 ctgtgacgcc gagtccgttc tagacctggc tcgccttgcg cagcgaaggg gcctatggct
    17941 ggtcgtggac gagacttttg ccgattattg cgaagagcga tcgatccttc ccaagcgatc
    18001 gctcttctcg cgggtcgtgg tcttgcgcag cttcacgaaa ttttacggat tgccgggttt
    18061 gcgagttggc tatgcggtcg cagccccagc cgtggtcgag agactgcggt cgcgcatgcc
    18121 tccctggtct gtgaatgcca tgggccaggt tgcggcggtg gcggctctca acgatgcagc
    18181 ctatgttggg aggagtctct ccttcatgcg gaatgaacgc gccaggttta ttgcaagact
    18241 agcccgattg ccgggatgca acgtgtttcc gagtcaggcg aatttcgtgc ttgtggagtt
    18301 gccgcaccgg tggggcgcca gtgcagtggc ggaaaagctt cgaccagaag ggctgttgat
    18361 tcgcgactgc tcctccgtgt caggcctgaa cacacattcc atcagagtcg cagtgcgaac
    18421 gaggcgagag aatgatcgct tggtgacggc actgtccaag ttgttggttc atcggtgacc
    18481 atatgagccc acgcagcaag gcgcgccttc gcactcgcta tcgagtcgtc gagaagacac
    18541 tggtgatcga tctcggtggc tttaaacggg tactgtcttc agcttcgcaa ggagggggat
    18601 ttcagtcggc tcggtacatt ctgaatcatc aagtcgaagc tcatccggtt tcaagggccc
    18661 atgctcgatc gaagcggttg atcgacccgg cgcggtacat gcgtcggctc gccatgacgc
    18721 ttggtgtcgg ggagaagccg attggtctca tgactgcagt tccgatgaaa cagctggtga
    18781 cttcatgtaa acgcttcggt gacacctggg tcgaatgttt tgcaacagtc ggggtgacga
    18841 atgccgtacg ggcaggggaa tggcctgaga gagagaacgg aggaaagcaa ggcactgatg
    18901 ccggaacgat taatctggtg ttggtcacga atgcctgctt gtctggtgcg gcgttagtgg
    18961 gggcggtcca agttgtgacc gagagcaaga ctggagtctt gcgagaccat gcagtgccaa
    19021 gttggactgg ggaatcgggt gcaaccggga cggggaccga tgtggttgtg atcgcgtgtg
    19081 ggctgcgtgg gcaggggcca tggcatcgct atagtggtac tcatacaatc atcggagcca
    19141 tgatcgggcg agtggtctct gattgtgtga ggcggggact agctcgggaa caacgatggc
    19201 gaagatctca aggatcactt aagaaatgaa ggctcaagct gttgcgatac ttggaacagg
    19261 atcagatgtc ggaaagagcc tgatggtcgc tggtctatgc cgcctgcttt atcgaggtgg
    19321 tgtacgtgtc gcgcctttta aggcacaaaa catgtcgaac aattcgtttg tgacgcccga
    19381 cggaaaagaa atcgggcgtg cccaggcatt gcaagcccac gcttgcggtc tcgacgctca
    19441 cgtggacatg aatccgatcc tcttgaagcc ggagtcggat cggcgtgccc aagtggtggt
    19501 gcaggggaag gtgtggagca cgtcagacgc gcaagattat tttcatcagc acaacgaact
    19561 ggcccaacgt gtacgcgaaa gctatgatcg cctggcggcg caatattccg tgattgtgat
    19621 cgaaggggca gggagtgcgg cggaaatgaa tctccgtgat cgcgatctgg cgaactggtt
    19681 ttcggtcgaa ctggccgacg cgaaggtcct gttggtggct gacatcgatc gcggtggagt
    19741 ctttgcgcaa gtgatcggca cgctggatct gctctcgccg cacgaacggg accgagtctt
    19801 cggcatcgtg atcaataagt ttcgcggcga tgccgcgcta tttgccgatg gggtctcctt
    19861 catcgaacag cgtaccgggc tgcctgtcct gggggtggtg ccgtttctgc gcggtgtggt
    19921 cttggaacag gaagacagtc ttgatatcga gcgtcgccgg cacagacgat ttgccgccga
    19981 tcgcgtgaat atcggcgtcg tcctgttgcc acgtatgagc aactttacgg attttgatcc
    20041 attggcagca gaacaggatg tcatcctgcg ctatgtcgcc gaaccgcacg agctggccgg
    20101 ggctgatgtc attctgattc ctggtaccaa aaatacgatc gaggatcttc ggtatcttcg
    20161 gcaggccggc ttcaccgata cacttcgcga gcatgtggct cagcggcggg aggtggtcgg
    20221 gatttgtgga gggtaccaaa tgttagggcg tagcattgca gatcccgaag gagttgaggc
    20281 tggcgggata gccgcaggga tgggatttct ggatgtgacg accagactcc tgaagaagaa
    20341 aataactctg cggatcgagg cagcgtcact tcacctcaat gacgggccag ctccttctgt
    20401 tcgaggttat ttcatccaca tgggtgaaac ggaacgcggg caggaatccg cctgttttca
    20461 tatacagtct gtgatgggtg tttcggatca ccaggagcga acattaggag atttgatgga
    20521 aggtgctgta agtgcagacg gactggtctg ggggacgtac attcacggaa tattcgatcg
    20581 gcctgccttt cgccgtactt ggctgaatcg cgttcgtcag cgtcgaggct tgcctcctct
    20641 gagcgtcgaa caatccgaag gagttactca acggctcctt gcagaactcg atcgttgggc
    20701 tgaccacctc gaacagcatg tgaacgtgag tctcatcttt aaggccatgg attagttgat
    20761 gcccacagcg atttctattg agttgggcgt tgacagtgaa agaggcgctg cgatgcgact
    20821 ctgcggcgcc tctttatgcg gcgcaatgaa tgctagaaaa ttcctcattt atttttgacg
    20881 ttcaccgagg attaccgcca ggcgattcaa cgtagcatcc tcctccctga gttgccgcaa
    20941 ttctcagagt tggctgagaa cgatccccgt atacttccgt tttcaacata gaacgtctca
    21001 tcgctgatat cgacctgccg atgggcgtcc accacggtcg ccccgccctc cgactgccgc
    21061 atggcagcga gaacccgttc ctcggtatgt tcaccttatc gtgccgtcgt ctgctcctct
    21121 ttcttggtga ataagaggac gccgctgcct ttcctttcgg tttttgggtc gcatcgaaat
    21181 gaggagtagg ctttcttcac tacatcatga acgagaatcg ccgctggtga cacttcggta
    21241 gcacgtttcg agggaagcgt tgccgtcatg gagtcgaatg aatgttgtcg gctacagaaa
    21301 catggagaag gtggtgtgcg gtggggaatt caaacagaaa acggaatgaa aaggctgttg
    21361 gtcaattttt gtccaaacgc catcattttg tcgcaacttg aacaatcctg tgccgaagtg
    21421 tggcgaatgg tactgcacaa gagaatgctt aactgcctga tacaaaatga atttccgcct
    21481 tggttttccg aaaaaggaaa acccagcgtc gttgagcacg ggctttgcag attggccggt
    21541 tgccttacgt ggtcatgagc gggtgaataa tcagggatca cgccggtttt gcaataaaac
    21601 cgatattgct aggcgattct gccagagagt gggaaaagag acgatgaatc gggagtaaaa
    21661 gcatctaata aggaggatgg gtatgaacgc gacacggtta agctggcgga tcttgagcgg
    21721 ggtggcagcc ctggcgctca gtgcaacccc ggtttttgct gggaacgaag ccgaagtagc
    21781 ggagcatttg atcgatctgg tgaagatcgg ccgtggcgtt atgtccgaac aaatgggcaa
    21841 tatcaatgac gcctcgaaag ccgacaaagg ctttaccggc gactatgtcg cggggcaagt
    21901 catcgagcga ttcaagaaga aaaccaagat cgacttacgg attcccaacg tcgtccccca
    21961 ggcaggcctc tatctcgcat tggtcgaagc ggaaaaggaa gtggtcgacg aagcccagcc
    22021 gatcattaat aggcctggta tcggattcaa gggttttatc cccgccgtct ttgctcgtaa
    22081 aaccggagaa catttctaca agaagtcggg tgtcaggttg aaactcacgg gaatcgacta
    22141 tcgcaatatc aacaaccggc ctgacgattt cgaggcggaa gtgttgcgga tgttcagtga
    22201 ttcccgccat cccaaggggc agtcctatgt gcgcaatacg atggtcgacg gcaagccggt
    22261 gctgcgtatg atggatccgg aatatgccgc ggcatcttgc ttggcctgtc atggtacgcc
    22321 aaagggggga aaggacatca cgggcggcca caaggaggga tggaaagaag gggatttggc
    22381 tggagcgatc agcgtggtgc ttccgctcaa gtaaacaatc ggaatcagaa ctcttaagca
    22441 gccagaggta tcaacccaga accaaggaga agcaacgatg agcaagattg tggtcgtaga
    22501 cgattcctat gccgaactcc aagtgattga aggtgtgctg aagggcgcga gccatactgt
    22561 ggtgtcgttt ccgaatactg agaagctcga agaaaaggtt gtcagcgaaa agcccgactt
    22621 gatcgtcctt gacgtcgtca tgcctggccg caacgggttc caggcttgtc gagacctcaa
    22681 gaacgacgat cggacgaaga gcattcccat tattctctgt acatcgaagg ggaacgaaag
    22741 cgacaagttt tggggccaac agcagggtgc caacgcccat atcgtcaagc cgttcaaggg
    22801 cgaggaacta ttggcagccg ttaagcgggt gctggggtaa cggtcatggc tgctgcacag
    22861 agctccacat cggtcatgcc gagcgtatct ggcaccgtgg tcccttcccc ggggggcggc
    22921 actgtgcaag caacgggcgc agctaccgtg cgggctgcga tcgtatcttt gggaggcgaa
    22981 ctctttacca ttgatttgca aaacgtacgc gaagtctttg tggtggaatc cattactccg
    23041 gtgccaggca tgccgtccgg cttggtcggt gtaacaaact tgcggggctc tgtggttccg
    23101 ctgctcgatc tacgacaaat gatcggcctc gctgccgaga ccacgcttaa gtatgccgtt
    23161 gtcgtgaagc atggcaactg gcaagtcggt gtcttggtgg atacggttcc agaaattcgc
    23221 actctgacga aggatcagtt catcccagct ccgaccggaa caggggatgg gggaaatccc
    23281 ttcgtgtcga cggtcgtgaa gttggaggac aggctacgtg gagttctgga aacctcggtc
    23341 gtcctttccc attttgaagg tacaggatag acaggagatc gcaatcaccg gacaccctac
    23401 ttacgggtcg atcgcatcgc tagcgagaag gaggaggttc ccatgctgag gctcaatgta
    23461 cttcaacggt tcgaaaacct aaaaacacag gcgaaactgc tgacggtgtt cgccctggtc
    23521 ggcgtcatta tcgcggccat ggccggtgtg ggcattgtca cgaacagccg cctggctgca
    23581 caggccgaaa ccgtgtatgt cgactacacg gtgcctctga ccgaattcaa cacgctcctg
    23641 ttcaacgtca accgctttca cgaagccttg attgacgtgg ccagaacgac gcgcgcttcg
    23701 gacttcgaaa tcgaagtgaa agaccttgcg ccataccgtg gtgaggtcga acggttaatt
    23761 gctaattatg aagcgacgac cttgcgcata tcaagcacgg gacgagatga agcaaaagat
    23821 cttgccgaac tcaaggatgc catcaaaaag ttcttcgacc agggcgaagc cgcggtcaat
    23881 acgattaagg atagctatga aagtaagtcg ttgacggcat cccaggccct cgccatgcgg
    23941 gatctaggcc aactcgccat tacggtaagt ttgtctcccg cctatgagaa ggtgacggag
    24001 cgccacgcag aatcgattaa gacgatggcc gaagttgcga aggatctttc cgagcagtcg
    24061 agatcgatgg cggcaaacgc cacattgatt ctgatcggag gcggtatcgc cgccgtgctt
    24121 ttagggttgg cgctcggtta ctgggtcgcg cacaagctgt cgctgggtat cggacaagtc
    24181 gccagcgtcg cgcagcaagc ggcggccggc aactaccaag cccgagccaa gattacgtcg
    24241 aaagacgagc tcggtcagat ggccagttcg ttcaatacca tgttagaccg cattacagcc
    24301 ctggtaacgt cagagggcga acgcgacgaa atgcaaaaac ggttgatgaa ctttctcgtg
    24361 ctggtgtctg acgtcggtaa gggagacctg acgaagcgcg gagaagtgac tgccgacatg
    24421 ttcggaaacc tggcggacgg gttcaacctc atgatcgcca ggttcgggca gttgctcaag
    24481 caggtccgtg aggcggccga acgcgtcaac aaatctgccg gtacgctgcg agacagcgct
    24541 ggtcagatgg ctggaaccgc caagcaccaa gcggacgagt cagttaagac gttgagcgcg
    24601 gtcgaacagc tggccgctca aatgcgtaag gtcgccgaaa cagccggtgc ttcttcggaa
    24661 tcagccaaac aggtgttgca agcaacggag cgcggtcgtg tcgcagtgca agaaaccgtg
    24721 caggacatgc agagcatccg ttctgcggtg caacgcatgt cgaagcaggt gaaggcgctc
    24781 ggtgatcgat cactggaaat ttctcaaatc gtatcgacca ttcgtgatat cgcgaaccag
    24841 accaacctgc tcgcgctgaa cgccgccatc gaggcggccg gcgcaggtga ggcaggcgcc
    24901 cggttcgcgg tcgtcgccga ccaggtcaga aagctggccg aaagctcgac gcaggcgacg
    24961 cgtgaaattg ccgaactcgt gaaggtgatt cagactgaga cgcaggatgc cgtggtcgcg
    25021 atggaacagg aaacgcaggc ggtggaagcg ggatcagctt ccgcgctgcg taccggtgaa
    25081 gtgttcaacg aaatttcagg catcgcgcaa cggtcagccg aattggcaca aaccatcgcc
    25141 agttccgcgg ccacgcagac ggcttcgacg gaccaggtcg gtcgctcgat taaggacttc
    25201 acgggcggtg ccgtggcgac gcagaaagcc actgatgccg cacgcatgac cgtcgaagac
    25261 atggccaagc tcgccgatgg tctgacggcc tcggttgcac agttcaaact ggcctaaggt
    25321 ctggtacccg ggggtgtcgc agcgacgccc ccgggtgaga aaggataggg atgagctccg
    25381 aactggaccg gcagggcctt ctcgatatct ttgtgactga agccgtggaa gccttggccg
    25441 tgcttgcggc agcggtcaat cctgccggcg aagcattgcc gagcgcacaa gagctccaga
    25501 accaatatgt gtgggcgcac aaagtgcgtg gtgccgccgg aatctatggg ttttccggcc
    25561 tatcgctatt gggagagctg ctcgaaaccg cgctggagaa ttccacgacg atcgatccgg
    25621 cgttgtggcc gaagacagtt ggtgcattgc gcgggatggt cgaggcgttt caagctcaac
    25681 tcgagatcgt caggcaaggc ggtcaggaag atcccactgt cggtgaacgg tggaaagcgg
    25741 agatggcggc gcttctgcca gcctcgcccg atacattgtc tacggaagga gctacttccg
    25801 accagcttcc tccagagtat ttggttccca ccattgacgc tgaagtcctg tcgtattttt
    25861 ctccggaagc cgacgaatat ctgcaaacgt tggaattgtt gaccaatcga ctccgagaga
    25921 atcccggcga tgcagaaacg attcagtccc tttatcggac cgcccatacg ttgaagggtt
    25981 cggcgcatac gatcgggttc aaggtgatcg gggatatcgc tcatcctgtc gaggcctgca
    26041 tgatcgcggt gcgagacggg caaattcctg tttccactgc tctcttaacg accatcggcg
    26101 aggctgtgaa cgtagttcgg ttgctgatgc gccgggacga tagcaaactc gatcagcttc
    26161 aacaggatgt gtcgggggtt acgcagacac tgcatcagat atgcaacggg gagccggttg
    26221 gcgcaccgcc cccctcgtct ccggccatgg cacaagacaa ctcggctcca caggtcgcga
    26281 cggccgtctt ggagccgcct gccgattcaa ctgagacgag cgagggctca acgtcatctt
    26341 cttcacctct gcaactgact gatgaatacc taatgccgaa actggatccg gaggtactgt
    26401 cctatttcgc accggaagct catgagtatt tggagtcgtt ggaggcccaa ttgctccggc
    26461 tggagaaaga atcggagaat tcggaactca tcaatcagct cttccgcacc gctcatacac
    26521 tcaaggggtc ggcttatacg gtcggttttc aggcaattgg cgacatgacc cattacgtgg
    26581 aagacttcat gggagcagtg cgcgacggcc gtgtcaaagt tctgtcgggc cacactgatg
    26641 tcttgcttcg gtcggtagac gtggtgcgtc tgcttatgcg tcgcgacccg accttgatgg
    26701 gaacattacg gcagcggttt gtcaccgcca tgcaggggct caagcaactg gagcagccag
    26761 cggcggtcga aacggtgacc aaggcggcag tcgctcagcc ggccccagcg ccggtcgtca
    26821 acgaagcacc cgaccagccg gaagctgatc aggcgaaagc tggcgatgcc agggccaaag
    26881 atgcgagcgg cgaggatcgt gaagtcatcc gtgtcagccg ggatcgattg gaacggctgc
    26941 tgaatctcgt cggcgaattg gtcatcggcc gcggacggct tgaacagcgg ttgcaagtat
    27001 tggagcagct ctcgcagcag gtgttggcgt gcaagggccg attgatggat tcggtgcggt
    27061 ctttcgagga gaaacacacc ttcacgcttc cttcgtccgg agccaactcc gcggatggag
    27121 cgaagtctgg ttcggtagtc ggagcggctg ggttcacggg attaagtgat ttcggaagct
    27181 tggagttcga caagtacgac gactttaata ttcttgcacg gcgaatcagc gaagtcacgg
    27241 ccgacatttc cgaatcgatg acccagttaa gcggatcgat tcgccgttcg catgaggaca
    27301 tgagccaatt gcagcaattg actctcggta tgcgggacga gattgcccgc gcacgaatgg
    27361 taccggtcgg cacacccttt acgcgattcc ggcgggcgac acgagagatg gcccggacga
    27421 cgggcaaaga ggtgacgctg gtgacgtccg gtgaacatac ggaagtcgat accggagtcg
    27481 tggagcgctt ggtcgatccc ttggtacacc tggttcgcaa tgcggtctat catggtatcg
    27541 agccagctgc cttgcgggtt tcaaaaggta agccggccgc cgggacgatc tatctccatg
    27601 cctcgcatcg tggtaacgcc gtgctgatcg aagttgaaga cgacggggcc ggtctggaca
    27661 tcgacaagat cagggcgaag gccgtcgagc gcggtcttgt tagagccgag gtagcgaaga
    27721 atcttccgga agctgaagtg attaagttca ttttcatgcc gggtttttcg actgctgagc
    27781 aaatcggtga tcaagcaggc cggggcgtcg gcatggatgt cgtcaaacgc gtcatcgaaa
    27841 gcatgaacgg acacatcgac gtggaatcca tccgcggcgt cgggaccaaa ttcactctcc
    27901 acctcccgtt gactctgctg attgcgacgg cgttaatggt tcggtccggc ggcgagcggt
    27961 acgggattcc gctgcccagc gtacgggaag tgaccatgct cacaaccggt tcattccagc
    28021 aaatgggcga acggtcgatg attcagatcg gggatgaagc gatcgatgtc ctgccgttgc
    28081 aacaactgct atccaggggc agcgccagcc gggtcgaaat cgggaacccc gtcgtcatcg
    28141 tgcgcaccgg cgtaggaccg gtcggccttg cggtcgatga gctgctcggt cgccaagaaa
    28201 tcgtcattaa gccgatcgga tcgctcaagt cgctgcagcg ttcgtgcttc ggcggtgcca
    28261 ccatcgatcc agaaggccgg gtggtgctcg ttctcgatcc tgcgcgattg ctgtcgggga
    28321 gcgagaaaac cgcggggtcg ttggatgtca cgaccgaggg aaccacatcg tccgataatg
    28381 agacgccgat tgatgaagcg cctgtgcaac ctgcggttga gcaaaaactg ctgctgatcg
    28441 atgattcttt gagtatcaga aaattcgtcg ggcgcatgtt ggaaacggcc ggctatgaag
    28501 tcgacacggc cgtggatggc gaggaagggc tccgcaaggc ttctgcgaaa aactatcgcc
    28561 tgatcttgac ggatcttgaa atgccaaaac tcaatggcta cgaagtcatt caagcattgc
    28621 gcagccgtcc gcagacgcag cagacgccga tcattgtcat gacgactcgg gcaggagata
    28681 aacaccgaca gatggcgctc aatatcggag ccagttcgta tatcgccaag cctgtggaag
    28741 agcgggcgct cattcaagag cttgaacgat ggattgggca ggagtctgcc gcacggaagg
    28801 cctgacggct ggactctcac tgagtcgcag gtaggctgaa tacgaaacag aattccctat
    28861 gagtttgcga ggacatcaca gggcgacgac tgccaccgtg cagacagcca gttttctggt
    28921 cgtgcggttc ggcaagaact actgcgcgtt gccgtcgcag ggagtgcgcg gtgtgctcac
    28981 catggaagag gcagggcgcg ctcagacggt caccgccgtg ggcgtgatct accaggatgt
    29041 cgatcttgcc gggcgtttct cgaccgttgt ggatctgact cgcccggaca cgcgcacgat
    29101 tctttattcc aacggtcagt cccacggagc cattcgagtc gaggaagtca tcggcatgat
    29161 tgatgtggaa cggcatcaat gcaagccgct gcccccgcat tttcaacagg acgaacggac
    29221 ttggatctcc ggaacgatct tttttcgcga gcagttggcg ctaattctaa atcctgaatg
    29281 ggtattgggt gaacttgggg aagaggtggc ggtcggtggg gatcgagaga agtctaaggc
    29341 gatctctcat gcgactggaa tcggtggaca atgctaaggg ctgcgcgtca acaggaccag
    29401 gccgtcgcgg caaagaaatc ttggaacgtc gtggtatttt ctgtcggtgg catgaaactg
    29461 gccgcgagaa ccgaggatgt cggcggggtg gcagcctggg ttgaaccggt ttcagtcccg
    29521 agccgcaccc cgttcatcgg tgcgatgctc aagagggaga aagacgtgat gccggtatac
    29581 gatctggcga ctcgtcttga acgcgaacca gagggcgaca cattgttgtg tctggtcgcc
    29641 cgccatatcg acggtcccat ggccatatgc attgactctg tgatcccgac gctccatacg
    29701 attgagatta gtcagattgt gccgagcaag cgcatggatc tcgaaacgct cggtagtttc
    29761 accgaagatg ggcgagacat cgacatcctc gcgctccaga ggctggggcg cagacgacaa
    29821 gatagcatgc ggccataagg tccaagctga aaggaatatg gacatgccga aggtgttagt
    29881 ggctgacgat agcattgccg tacgaaaggt cgcggaacgg ctgctgactg aggccggatt
    29941 gggcgttacg ttagcggcga acggtgaaga ggcattggcc agtctagcca aggaagtgcc
    30001 cgatttgatc gtgtcagacg taattatgcc ggataagagc ggatacgaag tctgtgcatt
    30061 tgtgcgtgct cagagcgcgt tggcaaacac accggtactg ctcatctcag ggatcgtcaa
    30121 tgacgaagtt accaagcagg ccgagtcctg ccatgcggac ggagtgctga agaagccctt
    30181 tcagggtacg tcgcttaagg atcgcgtcct cgaactgctc gccaagaaac acggtcaaaa
    30241 aaccgaaccg ccaaaacagg cggcgcagcc ggctcctccg tcgggggcgg agcgaggttc
    30301 gcgtgtcccg gacgagcaga tagcggcctc tcaacaaacc ctcgccaagc tgaagcaggt
    30361 tgaggaggaa cttcacaaag aacggcagca atcggcacag ttgactcagc gcatcgctca
    30421 acttgaaggc caggttggcc gtgtgaagga acttgacgcc atcgtggcaa gcgagaggga
    30481 gcaatcggcg caggccaaga aggctgctga ggaactggcg ccgatcgccc agcgagcaaa
    30541 agagcttgag gcgttgctga agcaggagcg ggaacaggcc gcgcaagtca agaagcaggc
    30601 ggacgatctc cgaggtcaag tgacacgcct gcatgaactc gaagaattgc tgaagcagga
    30661 gcgggaacag gttgcgcagc tcaagaagca gacggacgat ttccgaggtc aagttgcacg
    30721 cctgcaggaa tttgaagcgg cattgcaaca agaacgggtt aaaaccgcgc aactccagca
    30781 acgcgtcact gatatgcaga agtccgtgac gcgcgtgcaa gaactggaag cggccttgta
    30841 tgccgagcgt caggctgcca cgcagctcgt tcagcaaatg acggcgatgg aaaaagccgt
    30901 gagccgctcg caagagctgg cacaacagtt tgcgcgagag caacagcgag tggaagaact
    30961 gactcgtcgg ttagccgtaa gcgaagatgg aagcgcaaaa gcaaaagagg ttgaaaaact
    31021 gttggaaacg gagcgggagc ggaacgccgt gttggcgcat cgagtgtcgg aaaccgaagt
    31081 ggcggcgcag caggcgacca aacgattcga agagatggcc aagaaactcg gagaaattgc
    31141 aggtttggct tcccagttag ggaatggagc aaagcggtaa taatgattcg tgctcgggcg
    31201 ccacgggcca ggaggttgac ggcgtccaca tgggtccatt ctggtctcgc gttgttcaaa
    31261 gacgttggcc ggtggacaga gtaaaaacga atcgcttcct tgttgcgatc tcaattgacg
    31321 gatttgtgga tgcccttcgc gttgcgtgat cgactgctca tcgattcgcg caattcatgc
    31381 atttcggtcg cctctcggtg agcgaacagc gtgcatggat gaggatgtgg attcatgtcg
    31441 ggcgatacat cagatgaatt ccaaaaggag ctggttgacc tctttgttca agaagcccac
    31501 gagtggttgc agaatattca tgttgcgctc gacgagctgc agcaggcacc gccgcctgag
    31561 cggcacgcgg tgcttatcca gacgctgact gccggcgtga ccaacctcgg cggatccgcc
    31621 gccacaatta atctccccga tgtggaacag gccagtttcg ccgctctacc gttcatcgaa
    31681 gcgatcaagg atccccaaaa aaacttgtcg gtccaggact ttctttcgct gtgcaagcag
    31741 ttgggactca ttcataccgc gttgacgaat gccacgggtg tggcctttga tgcggaagcc
    31801 gaagtcgaag tcgtcgaggc ggccgcgagt ttgtcaccgg cagagtttct ccaatcgttg
    31861 cgaacgcttc agcagaatca aggtgcggcc acacaggggg acagaaatct catcaagacg
    31921 atgatcgagc aaatggaggg gcaagtccaa gccggtgtcc agcatgtggg cgtggacgtg
    31981 attcaaggat ttctggaccg catgtcggat tcggaagacg ccttcgtaca aagcgtcgat
    32041 gctcagatgc cgaacctggc agccaaactg tccggtctcg cagcatcagc cgatcccgcg
    32101 agtgatgtga tcgaatcctc gctcaaagag gttatgcaac tgagaaccga ggcccagcag
    32161 gtgaatgccg gaatcgcgat gaccttttta ggaggattgc aaagcctctt gtcggtcgtg
    32221 gcgcaacatc gtatacgtct tgcttccgcc agggttgaag cggtgacggc tcgtctgggt
    32281 gtcatgaagg aactcatcca tcagtgggtg gaacgaggac gagctgagcg gacggcgatc
    32341 ggtcatgtgc tgccggtgaa tcactgatga tcatccgacg acccgctcct ctctccagcg
    32401 tctccgcact tgactcgtac caccgtcgat tgcatgctga gatcaaattg tccaataatg
    32461 ataccgttcg agcatttgta taggcatagg gtatggaaga gcgacgcgca tctgacatct
    32521 accgcaaagc cgaagagcaa ctgactgcgg ttgcgacggc gattcaacat caggatcaga
    32581 tcgatttgga cggcctgtcg actttggcgg cggaaattgc cgactcggtc gaccgtgacg
    32641 atcaattggt cgtacaggcg ttagccggtc cgagtggctc cacgttggtg accaatttga
    32701 tcaacgtcag cattctggcc acgaaggtgg gaatcggcct gggctattat gggaaagagc
    32761 ttcgccgtct gacattggct gggctcgttc atgatgttgg actgttcgct gtgccgcagt
    32821 cggtggtcac aaagtccggc cggctgacgc gtgaggagcg gaccctgatc gaacagcacc
    32881 ccgaattggg ctatcaggtg atccggaagc tgggtggtga ttgggagtgg ttggcccgtg
    32941 cggtgcgcca agctcatgaa cgatggaatg ggaaaggcta tcctaaccgg ctcaagagcc
    33001 gcgatatcgg agagtttgcg cagatcatcg gcgcggtcga cgtcttcgat gcccttgtca
    33061 ctcccaggcc ctatcgtcgt cgcttctttc cccatgaggc ggtgcgtgaa ttgttggtgg
    33121 ctgagcgcac ggccttccct cgcgaagtga tcaaggccct tgtcgagcaa ttgtcggcct
    33181 atccgctggg aacactggtc cgactgacga ccggcgaggt aggcacggtc gtcaagatca
    33241 acacacgagt tccgcttcgt ccggtcgtgg aaattggagg gggaacaggg cagggaggta
    33301 gtcacgaatc ccggcaactg gaccttagtc tcctgccgct tgttgcgatc attgaaacga
    33361 tcgagtctcc gacagttgcg cgggtgccat ttccatccgg tatcgcagct gatcggcgaa
    33421 cgcaacctgt cccatccgta tccgaccagt tcgcctcgtt gctggaaagt ttggatgcgc
    33481 tggcgagtgc gatccaaggg gtcgtcgaga cacgggctcc ccttgcatcg aagggcgtgg
    33541 ccgatgcgca ggaatcggat caaggctggg taactccccg tccttcgggg cgcgatctgg
    33601 cagatgtgag ctttgaaaga gaagtggtcg ggctgtttgc gctggaagcg catgaatggc
    33661 tggcgcagat ctattctgcc ctgaaacggc ttggagaagg cacgaacgga tctgttcgcc
    33721 ccaagctcta tggcatcatg ttgcaggggc ttaccaatct ggccaagtcc gcggcgacgg
    33781 ttcatttgac atctatcgaa gatatggcct caagtctgtt gccgatcctt cacgatgtcg
    33841 ggcgacaaga accgcgatca atggctacgg cgctggcttc cctgcaagcg ggccttgatc
    33901 gcatcgcggc agccgtgcgt catgcggaag ggggaccaga acctgaaaag caggaggagt
    33961 ctgtcgctgg tgaaggattc cccgatcaca aagcggaaag agatgcggaa attcccatgc
    34021 catcggcgcc aacgctcttg gctccggtga tgggaaccac gggcgtgtca ttgttgaatg
    34081 ccttgcggga gcttcaacac gtccggtctc ggtcggtgca accggctcgc gacgtgttgg
    34141 agcccgttat tcttcgagcc gaacagcaac cggggattct tacggtcggc ggtgtccgcc
    34201 ggatactgga tgagctggat cgaatggatg agcggtttct cgaagaggtc aaccaacggg
    34261 ttcccgcagt gagccagctg ctcaacgatc tgcggaaaga cggcaccatg gactttgtaa
    34321 cggcttcgca gctcgatccc gtggtgcaac acgtcgaaga gctgcaagcc attgcgggat
    34381 cggtgcaggc cggcatgatg accatgttcc tagaaggact ccgatcgttt ctgctggtgg
    34441 cggcctaccg gaaggccgcc accctcccgc aacgattggc caccgtcgag gcgcgtatcc
    34501 aggcgctggt tccaatggca gaacagtggg tcagtatcgg ccgtgttgaa cgtggagcca
    34561 tcgctgaaat cctgccggct tcgtagagga ttcgttttat cctgccgatc acattcccga
    34621 ggcatattcc gttccatccc gtgtggcaag atctcgcagc gttgttcttc ggtgccaagt
    34681 tgttcgaacg aaaccacaca tcttattgaa aagtaatatt tttgattgcc caggacttag
    34741 gcaatctgcg aagcgctacg ccgtgaaacg gtattgagtt ttcatccaca tggttatcca
    34801 catgccatgc acacgttttg tggacagaaa aactcatgcc accggggagc tgaaaaaatt
    34861 tcgtcagact ggtcatgctg gcacgcaact agaacatcgc gaaagccttg gcggcaaggg
    34921 gttgcgcgaa cggtccgccg cttttctgcg atggcgtcac aaaacacgat atgtgaggat
    34981 gaaacctcag agatcaccga ggtcgttgca agagcgcgga tgaaatagcc atccagtccc
    35041 catcgattcc aggtgcagaa ggttggtgct tcaatcaatg cctccaagag accaagaatg
    35101 atatgatgcc tgacccaagt tcttgctggt gcagatgtcc tggccatctg tttgaagggg
    35161 gaagtgcagt gcctaaactg gtgaccgtat ggcgggagca agatatccaa gtacaggcac
    35221 gtcagtatgt ccgatcctat attctttttc cgtctggtct tttaggcctt gtctgcatgg
    35281 tcggtggtat cggttcgttg gggtatcaac tggtcgcatc aagcacctat acctggacaa
    35341 cattctggtc gagttccgga ctgttggtcc ttggcgcctg ttgcggctgg gggcagacgc
    35401 gataccatcg gtaccttctt gccaccttgc ctgaagtgtt cgctgcgcgc atacgcagtg
    35461 ccgtcacacg gaatcagaag aaaaggaaag gggagtcgtc gattcccgcg atcgaacatc
    35521 ctggtcgtcg tctcgtgccg gttgcctatg tccttggagg cgggctgttg ctggcagcat
    35581 cggcttgggc aatgttctac ggagccctgg acatggtccc cgccgtactg atgccgtggg
    35641 ctggatttta ttggggaaag ctcttctttt ggcgcggggt agtcagttga gtcggagaaa
    35701 aggccggttt cgactacttc cggcgtcgtt tgttgcgggc cggtggcggg gcagctgcct
    35761 gcgcgagttg ggattccaat gcagccacgc gttgttccag actgcggacg agctgccgca
    35821 attcaggaag acggggtatg acggcctgag ctttcatcca ggtttcatgc ggcattaccg
    35881 gcgcacctga aacgatctga ttcggttcaa ggctgcggtt cacgcccgcc ctggccgcaa
    35941 tcatgacctg atcccctata gtgatatgat cggccaagcc tgcttgccct ccgatcatga
    36001 cgtggtggcc gattgtcgtg ctgccggcaa tcccgacctg ggccacgatg atgtcatgtt
    36061 ctcctaccgt cacattatgg gcgatttgca ccaggttatc gactttggtg ccccgcttga
    36121 tcacagtttg accgagagtc gcacggtcga ttgtgacatt cgcgccgagt tcaacatcgt
    36181 cttcgatcgt cactcctccc aattggggaa ttttgtggtg ccgcccttca tgctgtacat
    36241 agccgaagcc gtcggcccca atgacggttc cgctatggat gattaccctg gcgccgagag
    36301 agcagccctc tcgaacgacg acattcgggt agagcacaca gtcgtccccg acgatcgagt
    36361 tgtcgccgat aaacactccc gggtacaggg tcactcttgc tccaatcctg acgcggtccc
    36421 cgattgtcac aaacggccat atcgaagggt tcgttccgat ggtgacttca gagccttgcg
    36481 tgatgttgtc cgacacgcct cgcggggcta cggtgcgtac gaaaaaggtt tgggcaacct
    36541 gcgcaaatgc gagcaacggg ttttggacca cgatctgcgg cgtcgcgatc tcgggaaaat
    36601 gacgatggac gaggagtgca ccggccacaa ttgccttcgg gttcttggcg gctttgtcgt
    36661 tggtaatgaa ggagagggct tgaggcgtgg cctcgttgag gctggcaagc gaaaacaggg
    36721 gagtgccgcc gtttccgtga agggtgccgc ctaccaattg ttggatcttc gagagagtca
    36781 gcgttgtcgt tgcggacggt gatcccatgg cgggctactt ctttttgttg cgcttggccg
    36841 cagtcttttt acgcggcgac gatgcgatgt tctcggctgg agcgagtcga ccctggacat
    36901 aaccggcgac ggctcccacg gttgtgaggc caggcaagtc ttgatccgga atctgaaggt
    36961 tgaaggcttc ctcgatctta tagagcaact cgatcactgc catcgagtcg agcccgagat
    37021 catcccgcaa gtggtgtgaa gccttgattg aggcggcatc gcgtttgagg tattccgcaa
    37081 gggcttgaat tattcttcct aggatcgcct gatcagtcat tatcccgttt cctcgttgcg
    37141 atggggtctg tccgtccgta gtcgtcaggc gacatagcgc ttaaggacga cagcagcatt
    37201 attgctgcca aatccgaacg cattcaggag cgccactttg atcttgcgct cttgaatgga
    37261 acgactgatc cctttcaaag cgcaggcagg atcaggatca tcgtaattgg cggttggatg
    37321 gatctgtcca gtctggatgg cgagcactga cgcaatgccc ccgatggccc cggccgcccc
    37381 gagcgtgtgg cccaccagcg atttcgtggc actgatggca atcttgtccg cacgcgattt
    37441 gaacagttgg cgaatagcct tggcctcgac cgcatcgccg atcgacgttg aggtggcgtg
    37501 ggcattgatg taatcgactt gtgccggtgt ggcgcccgcg tcggctaggg caagacgcat
    37561 cgtcgtcgct acttcctcgc cgtcttctcg tggaatgacc atgtgatagg cttcgctcgt
    37621 tgcggcatag ccagccacct cggcatagac atgtgcgtga cgtttcttgg cgtgcgcggt
    37681 agactcgagt atcagggcgc cggctccttc ccccatcacg aagccatccc gcagacgatc
    37741 gaaaggccgc gatgcctgtt ccggtcgatc gttgaatttc gtggaaagcg cccggagcga
    37801 acagaagccg gcaaaaacca gaggggtaat gctagcgtcg gccccgacca caataactac
    37861 gtcggcacgg ccctcccgaa tacagtgcag cccttgtccc acggcatgcg cgctggagga
    37921 gcaggcggtt gaaatggtga agttcggtcc ctttgccccg taggccatgg cgacgatgcc
    37981 ggaggcagag ttcagcgtaa tcacggggat gaaattcggg tgtacacggt gggggcgttg
    38041 ctgctggtag agttgggtga tctctcgttc acccatgacc atcccgccca taccagcgcc
    38101 gacaatgact ccgacacgat gcgggcgctc cttagccatc tggagtccgg aatcgaccag
    38161 cgcctctttg gcggcgacaa gggcaaattg cgcatagcgg tctactcgtt cggcttggac
    38221 gccgtcgata taacgttccg gagagaagtc atggatctgt ccagccacct tggagcggta
    38281 tccgtccatc gagaaggggg cgaaggtcgt aatggcagaa atcccggacc gtccggcgag
    38341 cgcggctttc cagaactcgc tgaccccgat cccgataggg gaaaccacgc ctaaccctgt
    38401 tacgacgact cgagcccgca tcccgtcttg ttctccagta aagaccgctt tcttacttac
    38461 cggaagcgct gtgatcagtc aaccagccaa tacatggtcg agggaagcgc ctccgatatt
    38521 ctcacgtcga cagaccatag ttcggtcatg cgcaattgac ctgtgagaga gagaatctgg
    38581 ggtcaatgac catggatcag tagcgaactt tgagcatcag atagagagca tagctgagga
    38641 agagggtgtt cgtggtccat ccggcgacca tcggagggag tgccccgccg cggccgaagg
    38701 cgattgcaat agaatgcgca gcccaataga agaagccgac catgagtgcc tggccaatgc
    38761 cgatggccat gctgccgcct cttgttccgc ttcgccgaag actgagagcg attccgacca
    38821 gtaccatgat aacggtgaca aaaggaaacg cgatgcgtcc ataataatca gtcagcagcc
    38881 gtctgaattg tgagccgttc tggtacaagc ggctggtgta gctgagaatg tcacgaaagg
    38941 tcatggactc ggattcccct gcgaacgctc cgacaaagtc gtcgggaagc agggtcaggg
    39001 gaatcgattg ttgttcgaaa tgcttgagtg tcacggtgcc atccggattg aagcgccggt
    39061 ggaaaccccg atacaacgtc caggcgtgat cggcaaaccg tgcttcttcg gcttcggcca
    39121 tctcgatcat gtgaaacgcg tggtcgaaac ggaagagttg caccccgccg agcgtgcttc
    39181 ccccaatggc cgcgctgctc acatgcaaca aggtgtcagc gtccaccctg gcccagggct
    39241 gggacaattt cagcgcagtg gacgggggtt ttttctcaat gcgaacgaca cggatctctt
    39301 cagcctcgtt cgaggccaaa ggaatgacga tcgagctaaa cagcaggagc accagggtga
    39361 tacaggcagc gaaggccaga aagggagacg taatccaggg cagactgatc ccgcaacttc
    39421 gcatggcggt gatctcatga ttgcgagaga gtaagccgag agtcagcaga gtggccatca
    39481 ggatggcgag gggggcgatc tgaaaggcaa tgcccggcat tttcagcaca aagtaaatga
    39541 ggatgtcgag ccactcggca tcgtggcgga ggaatcgccg taccctctcg aaaaattcga
    39601 tgacgaggta aatcgtcatc aatcccgcaa aacacatgaa aaagatcttg gcgtattcgc
    39661 gaagcaggta gcgaaacagg atcggcatga tgctattgtc ggctcatgtt gtaaaaccac
    39721 accagcgtga cgatggcaaa catgccattc gggacccagg ctccggcaaa cggagagatc
    39781 atcatggtcg tcaccagaaa ttcgcaggca acgttgaaga cgtaatagat gatcacgacg
    39841 agcaccccga cggcaaatcc gccgattctc cccgagcgct tcgagacaat gcctatggga
    39901 acgccgagga tacagaaaat caaggatgca gtcggaaatg ccagatcctt atagtactcc
    39961 atcagccggc ggcgcgcgcc aggatcacgc cactgtgact ggttaagttg tgccatcaag
    40021 gcgtcgtatg tgggccgttc atcggcagcg gcatacccgc tttgattgag cgagagcctg
    40081 aggtcgtagc tggaaaagga ggcgaattga tactggtcga ttgcatcggg gcggctgtgg
    40141 atcacgccat cctgcagccg caaggccact tgatcggtcg agctatccat cagcacttgg
    40201 tagtgctgtg ccacgatgat gcggggatcg tcggggtttc gttcatcaga gataaaaatg
    40261 cccgcggcgg acgcctccgt accctcgtcg gagacgtaaa tcatcatttt aggaatcggc
    40321 tcagtgaagg aaccccgttc cagggccaac actaactgat cacgaagtaa attgagagcc
    40381 actctcttga gattggtcga agtccagggt tgaccatatt gagccagcca tagcgtcagt
    40441 gcgaaaacca gcagggcaaa gaggaagact ggacgtccga ggcggaacaa actgaggccc
    40501 gcggcgcgca tggccacaag ttccttgtcg agagacaagc gaccgaacgc ggtgatcgac
    40561 gcgatgatcc cggcgatcgg aagggttaag accagaaatg aagggaggag atggacaaag
    40621 actttcatga cggcaaagat cccgacgcct ttggaaacaa gcagttccac caatcgcagg
    40681 agctctctgg tcaacatcac gaaacagaga gcgcctaggc taatgctgaa aggggagagc
    40741 agttcggtaa agatgtagcg atccagcagc ttacggagca gcatgagggg ctgtgttctc
    40801 ctggtttagc gcggcaatat agcaggatgg tgaaaaagtc cgccagcgtc ggtccccgca
    40861 ttgtccaaca gcacagggtg cgcagcgggt atatcgattg caccgctgca ccagctgcag
    40921 ctgacgcctc tcctatcagg ctccgtggtc cattgtccgt gcctgtttca attcgaccga
    40981 cgatcgatcc gtaaccttat aggggactgt tccggacttg tctataagtt gatgaatcgt
    41041 ccggacgcca aagtatggct tgacaagatt tgaaatccca tggtacatgc tgcgcgattt
    41101 gtaaccaaga tgggtttcgt gagcacttct ttttttctgc aaagagcccg cccatccgct
    41161 cctggtcctc gttctatttt tattccttct tcatgcaatc ggtgtgcgtc aactgggttg
    41221 ggcccggtca cgtaacggtg cgtgatcgtt gaggaaagga ggggtttcgt gaagacaaaa
    41281 ggtacggtga agtggttcaa cgatcggaag ggattcgggt ttattcgact ggatagcggg
    41341 gaggatgtct tcgtacatta ctctgcgctt caaggcgaag gattcaagtc cttgaaggag
    41401 ggagaaaacg tcgaattcga tatcgttcag ggggcgaagg ggccacaggc cgccaatgtc
    41461 atgaagatgt cgatcgccgc ctcgtaactc cttcaccgct cttgatcagt gcagaacgaa
    41521 ggcctgccgc aggaagcggc aggccttcgt gtttctagcg atcgtcatgc cgtgagtcga
    41581 ccacgacgat tggccatttc gttgacaaaa accattgata ctgttagcgt ttgttgaaca
    41641 ttggaggtcc gccttgggca tcgaccgtag taaggttctg cacagcgccc agctctttgc
    41701 ctcaaagggt caattcgacg ccgcaatcag tgaatggaag aaactttcgg ctgagtctcc
    41761 cgccgatggg agtattcaca attcaatcgg cgaactgcat ctaaagcgca atgccaccgt
    41821 ggatgcggtg aactcgttca ttcaagccgc caatgccttc cgtgccgagg gggcgaccct
    41881 caaggccatt gccacctaca agaaagtatt gaaactcgaa ccttcccgct acgatatgta
    41941 tcggcacctg ggtgatttga atgccgagcg ggggctgctg agcagcgccg ttcaggatta
    42001 cctgacgctg ggcaaacatt atctcaaaga gcgcaagacc aaagaagcgc tggaaatcta
    42061 caaacggatc gtcagtcaag acccatccaa tctcgatgcg cagcagcgcg tggccgaact
    42121 gtgtctgcag gaaaacatgc aggacgaggc gaccaaagtc tatctccagc tcggccgcga
    42181 gcggtcagcc caacagcggt atagcgaggc gaaagaggcc taccaatccg tcttgcgtat
    42241 cgatccgaat aacagcgaag cccaacaatt catcgaacac ttcgaaaaaa ccggcggggc
    42301 gccggcacga cctgccaggt caagcgccag cgccggagtc ggtgccgggg aatcgccgga
    42361 tttgctggcc gaggccagcc ggcgaattga ggacaagcag tatgccggcg ccgaggccat
    42421 tctcaatcaa ttgttgacca aggagccagg caatccccag gtgtgccaat tgctggcgcg
    42481 attacatctg caacagggcc aacttcaagt cgccttggga gaatatcgtt tcctggccgg
    42541 agcggccttg cgggcgcagg actatcagca ggccgaatcg ttaatcgctg aattccttgc
    42601 cgtcgagccc aactccgtcc cgttgttgga gctgcacggc gaactctacg aggaaaaagg
    42661 ggagattgag acggctgttc agcactatgg gaaagccgtc gaggtgctgc tgacccatcc
    42721 ggaaccgggc atgccgacgc tccacgaaga aattttcgag aaaatgtgtt cgttggcgcc
    42781 ggacagccca acggtcaaaa gactctccgg cctgatgagc ggagacacgg ctgccgtcga
    42841 accggccact ccggcaccgg cccaatctgc gccaaaggcg gagttttcca tcgaaggcgc
    42901 gacacccgac gatgcctgga gcaggacgac ggccgtcaag ccgttacagg accggcattc
    42961 tgttgaagcc ggtctcacgg tagagtcgtc tcaggagacg caggacgacc ccgcaacatt
    43021 ttccatcaaa aatgccgcac cggatgaaga gtggagcacg gttgcgcagc cagcctgtgc
    43081 gacagaaccg gagaaacccc ctctttcgac gctggaacgg ttcagggcgt atatggaagc
    43141 aggacagctg gtgcaagccg agcagtggct tggaactgtg gtgaatgcag aaccggacga
    43201 cgccgaagct cgagaattgc tgggacaggt gcttgaggcg aagggggatg gagccggtgc
    43261 agccttgcaa tatgcccgtg cgctggagtt gcgtacggcc cattctgaca gtgcggatac
    43321 caagcagctt tccggattgt acgacaaagt gaaggaactg gctccagcca gtccgatcgt
    43381 ggcgaaactc gcaggaatgt ttgcgccgcc ggcaccagcc gcagcacctc ccgcttccga
    43441 acaggcagcc gatgcacttg atccggagac gcattacacg ttaggggttg cctacaagaa
    43501 catggggctc ctcgaagagg caaaagagga atttgtgttg tcgatgaaag gctcggaagt
    43561 gttcgtggat tcctgcctca tgatggcggt ctgtctcaaa gaacaagaac agtttgaagc
    43621 ggcctgtgcc caactcgagc ggctgctgac cgatccgcgc agccagggag ccaaagctca
    43681 agccattcga tatgagttag gtctgctcta cgagaaacag gagcaatggc aactggccac
    43741 gacgatgttt gaaacgattc caactttcca cgacgtaccg caacgcctgt catccattcg
    43801 cgcagtgcct caatcgccag ccgcttccgc caaagtcttc cggtacgcga actaagcatt
    43861 cacgtccact cgattgggtg cgaggcgtcc gccgagcacc gcctcgatgt tgttgagaca
    43921 gatcatgccc atctcaactc ttgccgccat cgttgctgat cctaagtggg ggagcaacac
    43981 gacatgcggt aacgatacca actccggatg cagtgccggc tccttttcat agacgtcgag
    44041 tcccgctcct gcgatgctgc cattccgtaa cgccgctacg agcgcgcctt catcgatagt
    44101 cgcgccgcga gccgtgttga tgacaattgc actccgacgc atctgcgcca gttctcgcat
    44161 gccgatgagg tggtgggtat ccgccgtcaa gggcacgtgg atcgagacga agtcggatgc
    44221 gctgagcagt tccgggagtg ttctgcgttc ccacgaggcg gaagcgggcg gagggactgc
    44281 aggccgtctc gcatggtaga gcaccttcat gcgaaagcct gccgcccgtt gagcgacggc
    44341 ctgaccgatc cgtcccatcc cgataatgcc caacgtttta ccggcaacgg ccgcccccaa
    44401 taattgagtc ggcgcccagc cggtccattg gccgcttcgc acaagacgat cgccttcgac
    44461 cacgcgtcgt gccgtggcaa ggatcaaagc ccaggtcaga tcggccgtcg catcggtcaa
    44521 cacgtcaggc gtgttcgtaa caatcagacc acgtcggttc gcagcagcca gatcgatgtt
    44581 gttatagccg acggcatagt tcgccaggac tttgagtcgt ggagcggcgt cgatcgtttc
    44641 tgcatcaatc cggtccgtga gcgtcacgat tgcggcgtct gcctccatca ttccgtcccg
    44701 aagcgcaggg gcggtagcgg gagcatcgct cggttcctgc gcgagcgtga actgttcgcg
    44761 gatcatcgcc atgactggat cggggagcag acgcgagaca tagagcagag gcttctgagc
    44821 ggtcatggca ttcacatctt agcgagtcgc gtgtcgaccc tcaaccatca ccttcctccc
    44881 cgtcgcctgt acctgttcgc gatgcaaagg gaattcgtcg ggctcacctc ttcacctgct
    44941 ggcggacatt ttcaacatcc tgttagaatg cgccgccatg ctgatcatgc ccgacatttc
    45001 tcgtgcagtc gcatccgatc ttcgccgtct gctcggcagc gatttcgtca aggacgacga
    45061 ggcgacgaag accgcctacg ccgtggatgc gagcatctac cggatgacac cgcaggccgt
    45121 cgtcctggtg gagtcggaag agcatatcca aaccgtcatc cgatacgcgg tttcacgagg
    45181 tgtcggactc acggcacgtg ccgctgggac gaatctcacc ggttcggcga tcggttccgg
    45241 gatcattctc gacgtttccc ggcttcatcg aatcattgaa gtgaatcggg aagaacggtg
    45301 ggctcgggtg cagccgggga tcgtcttggc cgagttgaat aagcagcttg cgccccaagg
    45361 gttgatgttt ggaccagatc cgtcgagcgg ggagatgtgc aagctggggg gaatgttagc
    45421 gaataattca gccggccccc acacccttcg ctatggtgcg gtaaaggaca atgtggaggc
    45481 gcttcgtgtc tgtctccact ccggcgagtg ggtggatgcg cgcgactatg cagtcgacga
    45541 tcctcgtctc tccgcactcc tatcctctca tgcagccctt caagagacct ggtcgcttgt
    45601 ccatgcacag caggcgttgc tcgagcagaa gcgtcccacg gtcagcaaga acagttgcgg
    45661 gtacaacctc tttggattgg ctgagggatt gatgcgcggc cggttcgatc tgccgaagct
    45721 cttcgttggc agtgagggca ccttggggtt gttcagcgag gcgaccatca agctcgtcga
    45781 gaaaccgcgg gcgagtctga cggcgttgat tcatttcaag cgactggaag aggttggcga
    45841 tgccgtgccg aagttgctgg agttggcgcc cagcgcactg gaagtgatgg acgccaacac
    45901 gctcgatctt atcggtcgag caagacacgg cattccaact gacgcagctg cgacgttgct
    45961 ggctgagttg gacacgggcg gccttcagat cgatttgcgg gcccaggccg agcgcatggc
    46021 cgctctctgt cgtggctacc agctttcctc ggaggtcgtc atcgcctacg atcaggagca
    46081 gcgcgatcag ctttggaaag cccgcaaagc gctttatccc accctctatc gattcgattc
    46141 gcgaaagaag ccgattaatt tcgtcgatga cgtggtcgtc cgggcagagc ggatcagcga
    46201 gctcatgcgc tatctggaag acttcttcca cggccagcgc gtgccggtcg cgatcttcgg
    46261 acacatcgga aacggtaacg cccacattgt gccgttgctc gatgtgaaca atgagcagga
    46321 tttccagaag atggttcagg gctaccacga gattcatcag accgtgttgg accgattcgg
    46381 cggatcgatc tgcggggagc atggcgatgg tcgcgtgcgg gccgagtacg tcagcaaaat
    46441 gttcgggcct gaactctatg ccctgtttgt ccgggtgaag cgaagtttcg atccggcagg
    46501 ggtgctgaat cccggcatca agatcagcga ccgtcccttc accgagcata tcgactacac
    46561 caggctctca aaagcctgcg ccacctgtgc caaatgcaac gccgtctgtc cggtatacga
    46621 cgtattccgc tccgaggata tgagttcgcg tggctggttt gaaatcgtga ccgacaagaa
    46681 ctacagctac cttcaatcga aacgagtcgt cgaggcctgt ctcaattgta agtcctgccg
    46741 gactgcctgc ccggccggcg tcgatgtctc acaactgatc ctggatcggc gcgcggagca
    46801 tcccaataaa ctcgcaggtc tgatattcgc atttcatgcg caatcaatgc tgtttggcat
    46861 attcttaaag ttattagcga tatttcaacc gatctgggat cgcccgggca tcaggcgatt
    46921 cttggatatt gccacgaagc cgattctacg acatttggca gagacggcga gatggtctcc
    46981 tcaactggtt atgcccacgt tggctcgaag gcatcttcgc gaccgctatg cggggttggt
    47041 gccacaggtt ggtctgactc ctcggtccaa ggtcgcctac tttcatggct gcgccgccaa
    47101 ttacttcgat gacggcgtcg gagatgcggt catcgccgtg ctgagaaggc atggggtgga
    47161 gccggatctg ccgccgcagc gctgctccgg cacgccgatc gaaacctatg gtcatcgcga
    47221 gttggcccgg gaaggggctc gcgtgaatct tcaatcgctc gccgggtacg aaacggtcgt
    47281 gaccggctgt gcctcctgca cgctcgcact tagagattat ccggccctgt ttgccggtga
    47341 gcccgagcaa caggcggctc aggcgctggc cacaaaggta aagcatattt ccgaattcgt
    47401 ggcgcaatcg ccagtgaagc ccccgatggc gggtgttgca tcaacgaccg gcacggtcac
    47461 ctatcattct tcttgccatt tgcgggcggc gggtgtgaca aaggagccgc gccagttgct
    47521 gcagagcctg cccggggtcg agtacgtcga gatgcccgat gcagatcgtt gcgcgggcgg
    47581 ggcggggacg tacctcgtga aggacttcga tagctctcaa cgaatcgtgg agcggaagcg
    47641 ccgggcggtg gaacagagcg gcgccgggat ggtggccacg agttgcccgg cctgcatgat
    47701 ccagctgcgt acgggactgc atggcgcggc cagggtcaag cacatcgctc aactgttaca
    47761 ggaagcctat gatgctgcgg atccaaagaa agcgatgacc ctatgaagct tcgcaaggcc
    47821 gattggctgt ttgtattgtt cgcggtcgtc gtcgtggcgg cggtgtccat gttgccgacg
    47881 ccgaaagacc ggaatccgat gattccggca agcgccgagc atcgcgccat cacaaccgag
    47941 aaagagtgcg cgtattgcca tcaggccgat ggtgtgaaac cggtgccggc gcgtcacccc
    48001 aagcggcagg attgtttccg ctgccatgcc cgtgcttcga cgtgaggggg tacgagggca
    48061 aataagacgt gccgaggact cccggaagtg ctgagcgctg ggacgtaact gttgaacgag
    48121 caggtctgac gggtgaacgt cggtaactga taaaggacgc caaaaccatg gtcacagtgc
    48181 tgatattcgg gctgacgctg cgcaacgcgg tcggtgagag cgaacttgat gtcgaacttt
    48241 cgggagtgac gacggtgaag aaactcgttg aggccaatca ggaccggtta ggcgggctcg
    48301 caccgtacat ggcaaacaga gaagtgttga tcaccgtgaa caagaaagtc agcaccgatg
    48361 atacggcggt gaagtccggc gacgtcgtga aattctcgtt ccaatcgcgc acgtcatacg
    48421 acggcacgcg tgacattccg acctaaggca acagctcaac tcctacgtct gcgcaccctc
    48481 aacgcggctc ttgtattcca ggaagagctt gctatgtcga ttaccggtga gttccagcgt
    48541 caaggtacct tccgccgtct cccaagcggc atggtaatgc aggtgtccga tcatcacggc
    48601 gacaccccag tagtccggaa ccggctgata gagatcgtta tcccacacga tacgatcgtg
    48661 gagcggaatg ccataggttt cgattaacgt gcgtttcagc gcttcgtagt agccgatgaa
    48721 ttcatttaac ccgttcaaag gccgttgcag gacgatgtac tggcctcgca cgagcgattc
    48781 ccgcttgaac tcatagccct ccagcactcc atggtctgaa atattgcgga gataaatgag
    48841 cgtaccctgt tcgtctcgct cgatctcgcc ggcgacagtg gtccgaagcc tgtcaggggt
    48901 ctctccccag tgactatgga gattgtccaa gaaggcgagt gcttgcttcg tgacttccgc
    48961 ctgcagcgtg gcgtcatcaa tgtcgtcgaa aggcactccg gcatcgggag gtccgaagtc
    49021 aggcaattca gccagtccga ttgccgggat cgtcgcgatg gccaggataa ggaaggcaga
    49081 acgaagactg cgcatgggca gcaccttaga ttgcaacagg gggttcggtc taccctacga
    49141 aagtgcaggg ggtcagcgcg agtgagactt ggccttcgcg gtcggtggag ccgcgagagg
    49201 atcgtcgggc cagtgatgtt tagggtagcg gccacggagt tctttgcgca caccctgata
    49261 ggcgttggcc caaaagctgg cgagatcctt ggtcacctgg accggccgct tcgccggtga
    49321 taacaaatgc agcatgacgg gaacctgtcc gcccgccaca cgcggagtct ccttgcagcc
    49381 gaacatttct tgcagccgca cggcgaggac cggcaggtcc gaagtattat attcgaggcg
    49441 gacatgcgat ccactcggca cggtgagatg cgtgggagcc tgccgctcca actggcgttg
    49501 ctgctcccac gtcaggatcg catggagcgg tgtcgaaaga tcgagccgtt tcacgcggtc
    49561 cagggtggtg atgccctcca gataggggcc gagccattgg tcgagggtct gcagcaacgc
    49621 ctcatccgac agatccggcc aggttgcagc atcaccttca acccgcctga gaaattggac
    49681 gcgtgcacgc cattgttgca actcaggtgt ccatgccaaa gcgccaagcc ctgctcgacg
    49741 cactccctgc aagagcgccg tcgcaatcga tgacggatcc ggtttcgaga gcgcctgttc
    49801 cgacaacacc agcgcgccga gccgtcgttg tctcgaggcc aacaccgcct tggctcgatc
    49861 gtcccacgcg acggcttcgc tctgtacgat gtcgccggca tacagcgact cgatgtcctt
    49921 gagtccaatc ggtgcggcca gatcgattct cgcccactgt gttcccccct cgagatcggc
    49981 aatgaccaga tatggctccg acgcgagcgg atccgggctg gcgaagagcg cgccgcgtcc
    50041 gttcgtcagg aggtagcgtg cctcggttcc ggcctgacgt tgggcaatcc gatccggata
    50101 ggccaatgca agcaaccggc cggtatcatc gacatggtca tgagcaacgg gcttggccgg
    50161 tttcatcact cgacccaatt gttgccgcca gagcttggcc gttcgtttca cgcgctggca
    50221 ggccccgcga tccaccgtgg ctcccgccgc atgatccggc tgcccatgca gcacgtccag
    50281 ccggaggcga agatcggcgt ttcgccatcc ggacggcccg cggagaatgt ctcgctcgct
    50341 caacagcgcg gcgatgtcgc aagccagagt cgtcgactgc ggagagccag ccttgagcag
    50401 catgtgggaa aggcgcgggt ggagcggcag ctcagccatt tgttttccgc gcggagtgat
    50461 gcggcctgtc agatccaaag ctcccagtcg agtgagtagg tctctggcct gtgccacgga
    50521 accggaggga ggtgaggtga gccaggacag ttccgacgga tcactcgttc cccacagcgc
    50581 caactccaac accagcggtg cgaggtcggc atccagcatc tcaggaggcc ggcgcgccgt
    50641 gagcgacgcc tgttcctttt ccgtccagag gcggtaacag attcccggct ccagacgacc
    50701 ggcccggccc cgccgctgtt cggcagaatc ctgcgtaacg cggatcgttt ccagcctggt
    50761 gaggccggac cgtggatcga agcgcgggac tcgtagcaga cccgcatcga tgacgacacg
    50821 aacgccgtcg atcgtcaagc tggtttcggc gatggatgtc gcaagcacga cctttctggt
    50881 gccgggcgcg gccggcgcaa tcgccgcatc ctgcgcagct tgcggcaagt caccgtgcag
    50941 cggggcgagg tgaatggtcg gactcaggtt cgcatcgaga agcttccgtt cgacacgacg
    51001 gatctccgcc atgccgggca ggaagacgag caggctgcct tgatcctgtg ccagtgaccg
    51061 tctgatgatc tgtgtgacag cgacgtcgag gtggcctgag aggggttggt cgagatagcg
    51121 cgtttccacc gggaacatgc gaccttcgca ggtgatcacc ggagctccgc caaggaggtc
    51181 gccgaccggt ccgcaatcca aggtggccga catcacgagg aggcgaagcg tcggccgaaa
    51241 gaaccgttgt gtttcgaggc agagcgctaa gccggtatcc gcttggaggc tccgttcatg
    51301 gaactcgtcg aagaggacgg cgccgtagcc gttgagcgaa ggatcctgtt ggaggaggcg
    51361 ggtgagaatg ccctccgtta cgacttcgat gcgcgttgtt gaactcacct tcgtgtccag
    51421 gcgcatccga tagccgactg tggcgccgac tggctcctgc agcgtcgagg ccatacgatg
    51481 ggccgcagcg cgagcggcga gccggcgagg ttccaggaga agcagcttct tgccttccag
    51541 ccatggcgcg tcgaagagcg ccagcggcac gcgcgtggtc ttgcccgcgc ctggcggagc
    51601 cgtgagcagg gcattgcggc cggccgtcag cgtgtcgcgg agtcgtggca ggacatcttc
    51661 tattgggagt cggttcatgc tagggtttgc cgtcacgagg agactgtagc agaagccgat
    51721 ctagagaagg aatggcgaga gccactaagt cgatggtgga cgtacaacag gtcatcgagc
    51781 acatccggaa tgcctttcgc aacacggtgc atcccggcga cgcgtttttg cagggcagcc
    51841 aggaaggctg tgagccagga gaagcgattg cccccttcaa gggtctcgcc cactggatgc
    51901 aggtcgatcc tgcgattctc gatcccgctt acacggctct gagctttcta tcggagggag
    51961 gcttccggca ttttcttccc gcgttcttga ttgccgatct tgaaggccgc ttgcaaaccg
    52021 cagatccggt cttccatctc acgaacggat tctccgacaa ggtcgtgaag ttgccggccg
    52081 ggacgagggt ttacgaaaag acgatcggga aatcggcatt tgtgaatccc agacggtacg
    52141 gggcgatgac gtggcaggat catgcgcgcg gccagttgtc catttttgcg cgggaggaag
    52201 cctgtgccat cgtggcctac ctggaataca agcgggatgc cgatccgcac ggactcaatt
    52261 gcgacgagat caatgcggcc ctcgacggct tttggcggga tcgggccaaa caggcgccga
    52321 cgcaggaagc gctgcggcag tatttgcagt cggaagagga gtatttcaaa aatctcagag
    52381 tgtagggagc ctgtccctgc aggaaagaat cgatggcgat gtatcaatgg acgacgacga
    52441 agcccaccga agccggatgg tattggtttc ggggccaggc acacgaggcc gatcccttta
    52501 tcgtgcaggt tgatgaggtc gggcagtttc aatggccgga cgggggatat caggaggtga
    52561 agctggccaa aggagagtgg gcggggccga ttgaggagcc gggcgagtga gttggtgaga
    52621 caggtgctcc ctgtgctcgc ggaacgcgac acgcgggaac aatcggatgc ttaaatcatg
    52681 ggcggtgttc gttcgacgcg cgcacgatgg gagcaacctg tctcacccta caagaagagc
    52741 agctccattg gcctaggagg aaaaggaaag cgagcaagct tggaaggagt gtttgtgggc
    52801 ggagccgatt gaggagccaa aagaatagga agtgggtcct gtcgctgacc tcacaggaag
    52861 ccttcgggtt cggctcacgt acctcccgga gcgatccttt cgggcttcgt agggtggaag
    52921 ccttctggaa ttccctcagc cacggtcttc cgaccgtggt gctttggtca gttccagctt
    52981 tccatcccgc cttagcctcg gtggatacct cgctcctccg gtaaagttcg cgagcccgaa
    53041 ggcgttccgt gagatgaaaa ggagtgggtg gagcgcaggt cagcgccacc cgcgctggtg
    53101 aactctcaat cggccgaagg ggagaaagaa atacgagcga gcgtggaggg agcattcaga
    53161 agggacagcg ggcaggagtc ggtctttagt catcgaggaa gatgattgat gcgggaacag
    53221 ccggttgagc cacatgtgtt tgtcattgtc ggtgggacag gcgatctgac gcacaggaaa
    53281 ctcttgcccg ctctctacca tctgcgcggt cagggtgtgc tggaaaaaca gaacacgctg
    53341 atcgtcggag cggcgttgcc agagatgagc gaagaggggt atcgcctctg ggcctacgaa
    53401 ggtctgcaga agtctggcgt gcgccatgac aaggaactca gggcctggtg cgaagcctgt
    53461 atccattatc agtccgttca cgaggggaag cccaaggatt tcgaagcctt ggccgccttc
    53521 atccggcggt tggaacggtc gcgcagcatg ccggaaaacc gcatcttcta cctggccttg
    53581 ccgccagaag tagtcccgag caccatggag cggttggatc atacgggact tctgaagagc
    53641 cacggctggg tccgcgtcgt atttgaaaaa ccgtttggac acgactttca ttcggcgcgg
    53701 gcattgaaca ccacactgca ccgctacctg gacgaatccc agatctatcg cattgaccat
    53761 tacctgggca aagagacggt acagaatctg ttggcgtttc gttttgccaa cccgattttc
    53821 gagtcgctct ggaagcgtga tgccattgag agcatcgaaa tcaccgtcgc ggaagatctc
    53881 ggtgtcgagc atcgcggcgc ctattaccag caggccggtg ccctgcgtga tatggtgcag
    53941 aaccatctca cccagctgat gacggttgtg gccatggaag taccggtgtc gttcgatgcg
    54001 gtggccatcc ggagtgaaaa actgaaggtc ctgcattcta ttgcgccgat caacccgcac
    54061 gatgtcgtct tcggccaata cacggcctgg agatccggca gcgtgaatat tcctggttat
    54121 ctcgaagaga aaggcattcc gaaggattcg accacggaga cgtttgtggc cttgaaggcg
    54181 gaaatccaca attggcgatg gaagggcgtg atgttctatc tgaggaccgg caagcggctt
    54241 ccgcgcaagc tgacccagat tgcggtgacg tttcacgaag cgcctacgca ggtgttccgt
    54301 tcgctcgatc ccgggagcat tccacccaac aagttgctgg tcacgctgca gccgagcgaa
    54361 ggattcgcgc tctgtttctc cgtgaagacg ccgggacgcc cgttccgatt taccgaccac
    54421 gcactgcaat ttagttatga ggaggcgttt ggcgaactgc cggaagccta cgagacgctg
    54481 ctacgcgacg tgatgatcgg tgatcagacg ctcttcgtca gtgccgagtt tactgaaact
    54541 gcctggcgtc tctatgatcc ggtcattgtt ggtgaacata cggtgcacaa ctataccgct
    54601 gcgacgtggg ggccacgcga agcggacatg ctgctggaac ggaacggaca tcggtggcag
    54661 ctgggatggt aagcgtgcga tgtgcagggg gtaggagtga agtcatcaag aggaggtttg
    54721 attgatgggg cgttcgatgg gcgatccgat acagcgattg atgggtttga tgttcaaagc
    54781 gcatccttgg cacggggtct cgattggaga caaggcgccg gaggtggtca cggcctatat
    54841 cgagatcgtg ccgaccgaca cggtaaagta tgaggtcgat aaggacagcg gcttccttaa
    54901 ggtcgatcgc ccgcagcggt tctcaaattt ctgtcccgtg tattatgggc tggtgcctca
    54961 gacctactgc ggcgagaaag tggcagggct cttctccaag cgggcgaagc ggaagggcat
    55021 ggtcggcgac cgcgatccgc tcgatatctg cgtactgaca gaaaagacca tcccccacag
    55081 cgacattctg cttacggcga ttccgatcgg cggattcagc ctggccgacg gcggtgaggc
    55141 ggacgacaag atcattgccg tgatgaaaga agatgcggca tacggggact tcaccgacat
    55201 cggagactgt ccgatcacgt tgatcgatcg cctccagcat tatttcctca cctataagca
    55261 agcgccagga gcggtgcaac acaaagtaga aattaccagc gtgtacggcc gcgacgaagc
    55321 gttgaaggtt atccgtgcca gccacgccga ctatcgagag aagttccctg agctggaatc
    55381 cctctggccg aagcagatgg ccacctaatc acagaaaggt cacaaataca tcccgatgaa
    55441 gcacgatcaa aaccatccgc ccgctgcaac cgcaatccat ggattctcgc ccggcttcgc
    55501 cgcattggga ctcgaagcgt cgttactcac cacgctcgaa acgcttggat atgaagagcc
    55561 gacacccatc cagcgcgaag cgatcccgcc gcttctggcc gggaaggacc tgctcggtca
    55621 ggcggcaacg ggaaccggaa agaccgctgc cttcacgctg ccgctccttc aacggatcgc
    55681 gcacggggcc aaggccaaac cggcggcgct cattctcgtg ccgacccgcg agctcgccat
    55741 tcaagtcagt gaggccgtcc aacgctacag taaagaactg cgtaccagcg tgttggccat
    55801 ctatggcggc caagccatcg gccctcagtt ccatgcattg aaacgggggg tggatgtcgt
    55861 agtggcgacg ccggggcgtg cgctcgatca catcaggcgc caaaccttga agctgattca
    55921 cgtgcagatc gttgtgctcg acgaagcgga cgagatgctc gacatggggt tcgccgaaga
    55981 tctcgacgcc attctggagc agactcccgc gacgaagcag accgccctgt tttccgcaac
    56041 catgccgacg cgcatcaaat ccatcgcgca ccggcacctt aagcaggcag tcgagatcac
    56101 tatcgccaag gaaccggtaa aagccggcgc tgcgccgcgc gtccagcaga cagcctatgt
    56161 ggtcacgaga caacatcgtg gcgccgcact cgtgcgcatt ttagatatgg cggggcccaa
    56221 gtctgccctg gtgttttgcc gcacgcgcgt cgaagtggac gatgtcacgg ccatgctcaa
    56281 tagccgcggg catcgagccg aagccattca cggcggcatg agccagcccc agcgcgatcg
    56341 cgtaatgagc gcgtttcgaa gcggacaaac ggaactcctc gtggccaccg acgtggccgc
    56401 gcgcggtctc gatattccca gtgtctcgca cgtcattaat tacgatttgc cgtcgtctgc
    56461 cgaagtatat gtgcaccgca tcgggcggac cggccgtgcc ggccgcgagg gtgccgccat
    56521 cacgatcctg gatcctcggg agcaacggct tctccgcagc atcgagcagc acacgaaagc
    56581 cagagtgacg gtggcgccgg tcccgtcggt cgcagacctc ctcgcgaaac gactcgaacg
    56641 aaccagggca ctggtccagg aggcaatgca agcagacgat cttgacggat ttaagggcgt
    56701 cgtgaaagcg atggccgcat cgggcgatcc cattactgtg gccgccgcgg cgatgaagct
    56761 gatctttcaa gagcagggtg gagagcgggt cgaacaggag attcctgcca tgccgatccc
    56821 ccagccggaa ccctataggc cgatgcagcg actaaccggc cgtcccgatg accggtatcg
    56881 tggcgcacag gccagatccg gcgggcctca gccgatggcc aggcctggtc ggggcggacg
    56941 ggaaccaggg atggcgcggg tctatgttgg tgcgggacgg gaagccggca tcaggccagg
    57001 cgatctggtc ggcgccattg ccaacgaagc caaagtgaat tcgagcatga tcggcgcaat
    57061 ccagatcgaa gatcgctttt ccgttgtgga tgtgccggag gcgatggcgc aacgaattgt
    57121 tgaagtgctg gggagggcga gaattaaggg gcggaaggcg gcggtgcggt tgtttagaga
    57181 ataaggaagt gttgtggcgg gtcctgtcgg tgattgaacc cattcgtcct cgctccaccc
    57241 atcccccagt gggtcccttc cgctgcgggc acttggtgaa agccgcgtct cggcgcggca
    57301 ggggtcgggc gggtgagaaa gttgcccggg tcagcgccac ccgcctgtag aggtcgcatt
    57361 tgccgcatga ggaagaggac aaacgagcga gcttggaagg agcatgtgta ccgtcagaca
    57421 cgcgtatgcg aaagtagacc cagccggttt tgagaacgta gaatgtcgcg atactgtttc
    57481 ccatgccgat ccaaaccttt actgcacaag tcgacgcgat caaagatctc acgcatgacg
    57541 tgcgccagct cgacctgcgc ctgattgagc cgaacaccat cagtttcaag ccgggccagt
    57601 tcgtctcgtt cgagatcccg caagccacgc caggccgctc ggtgacgcga ccctactcca
    57661 ttgtctcgca acccagccga tcgggcggca tcacgctgtt attcaatctg gtgccaggag
    57721 gacctggttc cacctatctg ttcagcctca aggcagggga caggacacag ttcaaaggac
    57781 cggcgggcaa cttttatctg cgggaggatc cggggcgcga gctgttgttc gttgctaccg
    57841 gtaccgggat cgcgcccctc tggtccatgc tgctcgccaa tgcggagcgc gccgacccaa
    57901 gaccggcgac gttactctgg ggactgcgca gccagcggga tctgtactac cagaaggaac
    57961 tcgctggact cgttcgaacg aacaagacac tgaacgcgat tacgacgctg tcccgaccgg
    58021 agcctggctg gtcaggcgaa tcaggccgtg ttctgcggtt gatcgaatgg caggtctctt
    58081 cggtgaagaa tcttgccgtt tacctctgcg ggaacagcgc catgatcgtc gatgccacgg
    58141 ccctcctgca gaagaaaggg ctctgcccga tctatcgtga gaagtactat gatgacagtg
    58201 gatcgccgga agattgaggg agccccccta attctcccgt gccgcacctg cgtcgctatc
    58261 tcagtggaga ggggagcagc aagctcgaaa gtccacctgt gttgtgatgt cgcaggacag
    58321 tcacgcgagt gaggtgaaga tgaaaaagca agcgcgcaaa tcggtcatgg tcgtcaccgg
    58381 ctccacggca gccggctgga gaagggcggc cgaggcgatg gccgcggcct tcgactgtga
    58441 gctgcaccgg gtcaatctgt ccggggtcgt gagtcagtat gtcggtgaaa cggaaaagca
    58501 gctcaaccgc atctttgaaa aggccgaaga aagcggggcg attctgcttt ttgaggaagc
    58561 ggatgcgctg ttctgcaggc gcagtaaagt caaggatgcg cacgaccgct atgcgaacgt
    58621 cgaaaccgcc gcgctgttac agcggataga agcctatgac ggcctcgcca tcctcgcgac
    58681 caaccggagc gcgaagatcg atcccgcgtg gctccgtcgg gcgaaggttg cgaagccgaa
    58741 agtatcaaag aagaaggcta cggggagatg acggcggaag cgcctggctg gaccacccca
    58801 caaaatttat cctggacgca gcgtctccga tgaaaggacg aagactatgg cgacaaggca
    58861 attgactccg gccggcatca tgcgactcgg cctcggcttc tgggggtcga agaccctgct
    58921 cagtgccatc gagctcggtc tgtttacgga gttggcgaag ggtccgctca cgctgcaggc
    58981 gctgaccacc aggctgaacc ttcatccccg cagcgcgcag gacttcttcg acgccctcgt
    59041 ggcgctcggc atgctgaagc gcaccggtac ccgctacgcc aatacgtcgg agacggcgca
    59101 gttcctcgac cggaataaac cttcctacgt cggtgggatg ctggagatgt ccaatgcgcg
    59161 gctctaccgc ttctggggct cgctcaccga agggctccgc accgggaagc cgcaaaacga
    59221 agccaagacg ggtgaagatt tcttcggcgc gctctatgcc gatccgcagc agttggaggg
    59281 gttcctgaag gccatgactg gcctcagcat cggcgctggc cgcgcgattg cgaagaagtt
    59341 tccctggaag aagtaccgca ccttcgccga tgtcggctgt gcgcaaggcg gcgttgccgt
    59401 cgaagtggcc ttggcgcaca agcacctcac cggcttcggg atggatctgc ccgtggtaca
    59461 gccggtgttc gaagcttata cgcaggacaa gggagtggcg tcacgggtga aatttcatca
    59521 tggcgatttc ttcaaggact tgctgccgaa agtcgatgtc atcatcatgg gccatatcct
    59581 ccacgactgg aacctcgacg aaaagatgat gctcctgcgc aaggcctacg atgcgctacc
    59641 accccacgga gcgttgatcg tccacgaagc cctcatcgac gatgcccgca agcagaatgc
    59701 gttcggcctg cttatgagcc tcaacatgct gatcgaaacg catggcggct tcgacttcac
    59761 cggcgcggat tgccgtaagt ggatgaagga agtggggttc aggcggacgc aggtggaacg
    59821 gttggtgggg ccggatggga tggtcgtggg ctataagtga tggatatgtg atgtggtctg
    59881 gtctggtgag tccctgtgct tgcggagcat cgacaataga atgtgagaac agcgtccgca
    59941 tggtcctgtg ctcccggagt gcgcacgcgg gactcatcgt attctcagat taggggcggt
    60001 gctccctcca cgggcgcaat tggaccattg cggacgctgt tttccgagat taaaccagac
    60061 aagttcggtg ggaccattga tatggtggtt gcgcgcgaaa ccttccatcc gcaccccttg
    60121 aacaggagga gaaacgagcg agcttggaag gaacattata agggctcgat ccttcatgca
    60181 agccgctgag aaagcctcct ccccctcact tgatgggata agggaagagc gaacttggaa
    60241 ggatcattga gttcgtggat gcaatccagc ccgcgtgcca cgagacaatc ttgacatcaa
    60301 cgctcggctc ccagtacaaa atcatgcttt gtctatatgg gaattattca taaaggaatc
    60361 attcatggag cctcttgccc tccctgcatc ttccaaagcc atcgtgatgg tcagtccctc
    60421 ggagaattgc gcgcgatggg tcaatgtcga tctgcgattc gtgatcgcca cgatcgtcgt
    60481 tttcgccgcc gtagcgacca ccacgatccg gttttcgcat gaagtcttcg acccgctggc
    60541 cgattcgcta atgatccggg gctgggctgc agtgattgct cggccgagcc tgctctggtt
    60601 caccatgggt atgctgctca tcgtggtccg gacgttgctg tggcttcgct accatccgtt
    60661 cgcttccgtg agcgcagaag aggcgccgcg gatgtccatc atcattccgg cgtataacga
    60721 aggtgcgatg gtcagaaggg ccgtcgatgc ctgtgtccgg gccgagtacc cgcgggaccg
    60781 cttggagatc atcgtcgtca acgacggcag caccgacgac acgggggcgc acgtcgaccg
    60841 ggccaggcgg gactgccccg atctcgtgca ggcagtccat ctgcccgtca acgggggaaa
    60901 acgcgaagcc ttggcagcag ggtttgcgcg tgccacagga gagattcttg tcaccgtcga
    60961 ttccgacagc ttgatcgaac ggggcgcgct gctcgccctc gccggtccat ttcatgatcc
    61021 gcgcgtcggc gccgtggccg gcaaagtgtc cgtgctcaac cggttccagt cgatcctgcc
    61081 ccgtatgttg cacgtacggt tcatcctgtc gttcgatttt ctccgcagcg tccaatcatc
    61141 gtacggcacg gtctactgtt gtcccggtgc cctctccgcc taccggacgt cggtcatcaa
    61201 acctctcatt cccacgtggc ttcgccaacg tttccttgga agcgactgca ccatcggcga
    61261 agaccgggcg ctgacgaacg acgtgctcgc ctccggctac caggcggtct accagcgcac
    61321 tgccgttgtc cacaccctcg tgccggaaag ctatcaaaaa ctctgcaaaa tgtttctgcg
    61381 gtgggatcgg agctacatcc gggaagaaat ccggttatgg aagatcatgt ggaaactgcc
    61441 gtttccttcc ttcgtcctta cgatgctcga aaccaccatc accaaccttc gctatcccgt
    61501 cgcctatgcc tcactcttct tgatggtcta catgacgatg caggacccct ggacgatcct
    61561 gcgcctgctg ctctcgatcg ggctcgtctc ccttttctat acgctgtact ttctgcacag
    61621 cgaacgttcg cgcgagtttc tcttcggcat cgtctacgca tacttctact tcgtaagcct
    61681 tctctggatt tttccctatg cgctgatgac cgtacgaaac cggcggtggc tcacgcgcta
    61741 agacacacgt gagtcacagc aaggcccgca ggatgagtat cctgcgggcc ttgtgctatc
    61801 tggcatcgac aacttcagag acagttatgc cggacagcgg cttcggcatc caattctgtg
    61861 agctgccgga tcgggtcgca ctaccaagca taggaggggt gtcgggccga ttccgatcgt
    61921 ccacacgttg acagctctgc caggggatcg tagcctagat tcctccaatc cagtaaaacg
    61981 ggaggaactt ccatgaaatt cctcaaacct gataaagatc tctggtccga caatctcgat
    62041 cggcggcagc tcttgaaagc cctcggtgtc gctggagcgg tcttggctct cggaggccgc
    62101 caaacgctcg cagagatggt ggtgccggtg tcagaggcgg ggggtgctgc ggccggagcg
    62161 gttccgacac gggccttagg caaaacgggt atccaggttt ctgctctttg tttcggcggc
    62221 gcccattggg gccgtaccaa agatgacgcc gaggccattc gcatcctgca cgaagcgatc
    62281 gaagcaggga tcacgttcct cgacaacgcc tgggaataca acggtggacg atccgaggaa
    62341 ctcatgggca aagctctcca aggccgtcgg cagcaggtcg tgctcatgac caaactctgc
    62401 tcgcacgggc gggacaggaa agtcgcgctg caacagctcg acgactccct ccgccgcctg
    62461 aagaccgact acctcgatct ctggcaaatt cacgaagtca tctatgaaga cgatcccgac
    62521 cggcacttcg tacctgaagg tgccgcagat gccctcctcg aagccaaacg gcagggcaag
    62581 gtgcggttcg tggggttcac cggccataag catccgagca ttcatctcaa gatgctggcg
    62641 cgcgactttc ccttcgacac ctgccagatg ccgttgaacg tcttcgacgg gacctacagc
    62701 agtttcgaac aacaggtgtt acccgttctg catcagcgag ggattgcacc gctcggaatg
    62761 aaaagtctca gcggaaatgc cgaagccgtc aagcaaggca tcgtcactgt cgaggaagcc
    62821 cttcgctatg tcttgagtct gccgattgcg tcgctcgtca ctggcatcga ttcgcgcaag
    62881 gtcctgcaac agaatctgga catagtgcgc cgatttaggc cgatgacggt tgccgagatg
    62941 gaaacgctgc gtgcgcgtgt ggcccgctac gcaaaagacg ggcggttcga gcgcttcaaa
    63001 tcgacgaggg attacgacgg ccgcgtcgga cgggaccaac acgggttgtc gtaaccttca
    63061 tcagcttgca ggagtgggca acgccaaacc atcctgctcc tgttaattcg gcttttggag
    63121 cgggttgctc caactgtaac ttgtgacgat gctggtcccg gatatcagcc gagcagagcc
    63181 gaggccagcc ggggcagcca gggaagcagc gccacgccga cacagatcga cagcgccacg
    63241 atcgaggcga cgagatcttc atccaggccg gtttccgtcg caaagatcac agctgtcacc
    63301 gcagacggca tcccggcgat cagaatcacg atcgcccgtt ccattccagt caggtccagc
    63361 agccaggccg ccatcaatcc cagcaagagg ccgccggcca tcctcactga cacccccagg
    63421 caggtcagct gccatgtccg ccgcaacgca tcaagactga ttgaaaggcc aagcacgagg
    63481 ctcgcgagtg gcatcgtcgt gaggtgcacc ggtgtcagtg catcgtggag ccatgagggc
    63541 agatgcaggc ccgcagcctt caacgccagg gtgatcgtta aggcccagag cggcggtgcg
    63601 gagagcaggc gagccagcga tgagcgtgcc gacggcgaag ccgtgccgtg ccacaccgcg
    63661 agggcataga ggaccgtgag cgtcaatgtg gtttgcccga ggtcgaagag aatcgcctgt
    63721 gcgagtcctt cctcgccgaa cgtggccagc gtgacgggat aggcgaaata gaccgaattg
    63781 atgcagccga tggagagcag caagctgccc tgggtcggac gagagagacg cagcaggcgg
    63841 acgagcggcc aggagagcag caccaccggc agcgtcacaa gacaggcggc cagcggcagt
    63901 ttccaagccg tcggcgcaaa gctcacgcgg tccagtgaca caagaatcgt ggctggcaga
    63961 ctgacagaaa acacgaagct gcccagccgt tccgcgtgca tcttggtgag cattgcatag
    64021 aaacgaagcg aggcgccgag gaggaagaga acgaggaagg cgtggaggga agggggcatg
    64081 ggacgggaga gtatcagaga gaggggggag gcggccagga gagcaggaag agaggatgga
    64141 gtgcccgcac gctccagtgt ctcagctcga ccgcactaag cttaccatct gctcggctgc
    64201 ggaacttgtt ccatccgggc tgcgcaggat ggaacggcga acagtcctcg ccgtcgcgca
    64261 acgcgaccgc atctgctcag caagtgcggg gcccgagctt tgacacaagt cgcgtacaaa
    64321 accctccatc ctctccgccc ggcgagagaa gaaaaaagag caaccttgga gggagcattg
    64381 aaatgtgttt ttgcggaagg agcagactga gccgatgacg aagcttaggt caacctcttg
    64441 atctcgcctt ctgaagagac gagcggaatg agagaccctt cagctcactt gcgagaaaag
    64501 ctcacacaca agcctggaag cagcaccatc agggtcggac ctggaaggtc tttggcccct
    64561 ttttgtggtc cgacaggtaa atcatgtagc gacctccggt gccgcatgtc accatgattt
    64621 ctccacgccg gctcaagcga tcgatttcga gaaacacctg attccaggtg aactcgggta
    64681 gacgttcggc caatgtatcc aggtcacaac catgcgtacg cttgatcgtt ccgaggatgc
    64741 gatcggtgac cggtgtttgt ggatccatga tatgaccctc ctgtgatgtt cgaacgctca
    64801 ctgcaatctt cctcacgccc cttccggtta cggatcgctt cccgatcgtg ggtagacgat
    64861 gccgaactgc tcatccggca tgagccgttg agacagatcg gccagctcct cggctgcgtc
    64921 gtcgcccaac ccgtaggcgc gactagtcag catgctgaga aaccggccca tttcccgtct
    64981 ggcccgccat cgatccccga gagtgaccgc gccgcggatg cgatcgagtt catcggcata
    65041 gggggcgaaa tcgtacgtcg gatatttctt cgtgaagtac gtcatgagcc ggtgctcgag
    65101 gtcatccacc cactcgaccg gcggagcgag acggtcagtc ggtgtagcga caacgagcag
    65161 aacggaagga gtcaccgtgc cgtgaatgtg cgcaggtatg agaaggaaga agacagctga
    65221 caggagaatc agcgccaaga ggggaatgac cgtgcggcgg ctgaaaactt ccatgaggac
    65281 cctcgttcca aaggatccgc atccaaatgg tgatgcctga cgttagtgtg tccggtgtgg
    65341 gagtgaaggg ggctgcagcg aagtccgtca tgcaggatga cagaacgtag catgcagaag
    65401 aggcggcagc caccgccact ggaacgtcag accagcatgt tacgccggtg gagattcttg
    65461 gtcaaccgtc tctcgatgag ccaagggagc cagtttccag ggattgctcg gcgccatgga
    65521 gcaacgagcg ggcttggaaa aagcatccga tgcacgcgcc gcacgcaact ccctcatgat
    65581 gtactcatcg gagcatatcg tgcttgagtc agcttcaagt cgtcttcata atgaccggca
    65641 tggatagagc ggagctcttg tcgttgtatc gtcagatgtt gctgatccgc cggttcgaag
    65701 agaaatcggc ggagatgtat gccctcgcga agatcgccgg cttcctccac ctctatatcg
    65761 gcgaagaggc gattgcagtc ggcgccatgg ccgcccttcg accgaacgat tacgccatca
    65821 gcgcctaccg cgaccatggt cattgcctcg cgcgaggggc cgatccaggc aaggtgatgg
    65881 ccgagctatt cgggaaagcc accggcctct gcaaaggcaa gggcggatcg atgcatcttg
    65941 tggatcttgc gcaccggttc atgggcggct atgccattgt cggaggccac atcccgctgg
    66001 ccaccggcct ggccttcgcg atcaagtatc aggcgctcga cctggtcacg gcctgctttt
    66061 tcggagaggg ggcggtgccg agcgggcagg cgcatgaggc gttcaatctg gcggccctct
    66121 ggaaacttcc agtgatcttc gtctgcgaga ataatcgcta cggtatgggc acgcccgttg
    66181 aacgggccat cgcgctctat gcggacgtgg cggagacagc ccgttcctac gggatcacgg
    66241 cggagcgtgt ggacgggatg gatgtgctcg cggtgcgctc gtcgatggac aaggcggtga
    66301 agcaggtgcg tgctgggcgc ggcccctggt ttatcgaggc gatgacctac cgatatatgg
    66361 gccactcgat gtccgacccg gcgcacggac attaccggac gaaagaggaa gtggaggagc
    66421 atcgcaagcg cgacccgctg ctcttattga aggaatcgat ggtgaatcag aacctgggta
    66481 ccgaagccga tttcaaacag ctcgagcggg acgtgggtga ggtcgtggcg gcagccgtca
    66541 aattcgcgga agacagtccg ttcccgagcc cgtcggactt atacgacgac gtgctgcagg
    66601 agtaagacgg ttcatgttgt tgtcctatcg ggaagccctc aaccaggcca tgcgggagga
    66661 gatgcgccgg gaccagcgca tcttcttgat cggcgaagaa gtcgcctact atcagggcgc
    66721 gtttaaagtc agcaaaggat tcgtcgagga attcggcccc cagcgggtga tcgatacgcc
    66781 gatcaccgaa gccggcttca ccggcctggc catcggtgct gccatggcag gactccatcc
    66841 gatcgtggaa ctcatgacga tgaacttcgg cattcttgcc ctcgatcaga tcgtcaacaa
    66901 cgccgccaaa atccgctaca tgtccggcgg acaactctcc gtaccgctcg tcatccgtgg
    66961 tcccgggagc gccgcccacc agttgggggc ccagcactcg caaagtctcg aagcgtggtt
    67021 ctgccatgtg ccgggcttga aagtgatcgc gccggccacg ccgcacgatg cgaagggcct
    67081 gctcaggagc gcgatccggg atcccaaccc cgtcatcttc atcgaagcgc agttgttgta
    67141 cggcacgaag ggcgaagtcc aagagggcga gtacacgatc cccatcggcg tagccgaagt
    67201 gaaacgggtc gggcgagatg tgaccatcgt cgcctattcg aagatgctct tcctcgcgct
    67261 ggaggctgcc gatcaactga gtcgagaggg catcgaggcc gaggtgatcg atccgcgcac
    67321 gctcaaaccg ctggacctcc agacgatcgt cttgtcagtc aagaagacgg ggcgtctcgt
    67381 gatcgttgag gaaggctggc gcttttgcgg gctgggggcg cagattgcag agagcgtcta
    67441 ctcggcggcg ttcgatcatt tggatgcccc gatcatgcgc gtcgcgggcg aagaagtgcc
    67501 gatgccctat tgtcgcccgc tggaggatgc cgccatcccg gacctgccgc gcgtgatgga
    67561 ggccgtcaaa tcgcttctgt gacgagagga gagccatggc gagtcgcgtt gtgatgccca
    67621 agctgacgga cacgatggaa gagggtgtgc tcgttgcgtg gaagaaacgc gagggggatc
    67681 acgtgcagtc cggggaggcc ctcgctgaaa ttgaaactga caaggcgatc atggatctgg
    67741 aggcctttgc ctcaggcatt ctgcgcaaga ttctcgtgaa tgatggagaa acggtccagt
    67801 ccggaacgtt gctgggcgtg atcgccgagg ccgatgaaga tatcacctcg gccttgaccg
    67861 acacagtcac ggccgccccg tcgaccggcg ggggaggcaa gcccaccgcc ccagcacctg
    67921 ccgtcactcc acccacgtcg cccacgcgca cagagggagc ccgccccatc gcgtcccctc
    67981 gcgcgaaagc tctagccgcc gaacgaggga tcgatctctc caccattacc ggcagtggtc
    68041 ctggcgggcg aatcgtcgaa gaagacttgc cgaagatacc ggcacccgtg acggcggccc
    68101 tgcccgccgg aaccgatcag cccctgaccc aaatgcggaa ggccattgct cgggccacga
    68161 cccagagcaa agcaccggta ccgcacttct atctgacgag ggagatcgat atggaggcgg
    68221 cggagcagtt ccgcgagcaa ttcaagcaac gccgtcagcc ccatccctca atcaccgatc
    68281 tgctgatcaa agcggcggcc atagcgctga cgaggcatcc cgcgatcaac gtgtcgtatg
    68341 tcggcgaggc gatcagacac tatgcgcata tcgatatcgg tgtggcggtc ggcatggacg
    68401 acgggctgat cacgcccgtc attcgcgatt gtggcgcaaa gacgctggaa gccatctcaa
    68461 cggaatcacg tgcgctcatc gaacgggcca aacagaagcg gttgcaaccg cctgaatata
    68521 cgggtgccac gttctccgtt tccaacctcg gcatgtacgg ggtcgacaac ttcattgctg
    68581 tgctcatccc gccacaggcc gcttcgctgg ccgttggcgc cattcgggag gtgcccgttg
    68641 ttgcagcggg tagtgtgaag attggccgac gaatgcaggt tacgttatcc tgcgatcacc
    68701 gggccatcga tggcgtgatg ggcgcggagt ttctgaaaga gttgacgcgc atccttgagc
    68761 acccgcaaga actggtcatc ccagtgagga catcctaaga tccaggtaga aagggcgtgg
    68821 atgaaacgcc tgcacgctcc gatgcctcag atcgaccgca ctaagcttcg cacctgctcg
    68881 gctgcggaac ttgttccatc ctttgcgatg cgggatggaa cgtcgaacag tcctcgccgt
    68941 cgcggaacgc gaccgccgct gcccggcaag tgcggagtcc cgagcttcgg tacaagtcgc
    69001 gcgcaggact ttccatcccc gccacttggg gagaaaaacg agcgagctct gagggatcat
    69061 tctagttgga tgaggtgtgc ggtaaagatg gtgatcatca gatgagaaag gagggcaggc
    69121 catggcgtgg ggacctgatg cgaatacaga agatgaactg gaaaagctgg cagctgtgcg
    69181 tgcgtatctt cacgaacagt tcccgggtgc ctcgattcgt gattcgtacg atgccggcag
    69241 gatagcggag gtgtttcgga tcgaaaccga cggcgtcgag gggctgcgcg atgcggtcgt
    69301 ctcgaccgaa tttctcgacg agtatccgcc tgaaaaaatg ggaaaggcgt tgaccggctt
    69361 gcgggttgcg gaacacctcc gctcagcgaa gggcaaggag gtgctggtca caagttgggg
    69421 tgtagaagaa aagtagggtt gaatccgttc ggccttcgct tatcgacctc tgggcgcgat
    69481 ccttccggat gcgaccgggc ggaagtcctt ctggaattcc cgcagccacg gttgtgccaa
    69541 ccgtggctgc gtctgtcagt tccacctttc cttcccgttc acaccccgct tcttgttcag
    69601 gtccatgagc gcataacttt tgtgccggcc cccgagcagc aagtcaacgt gcacattcac
    69661 ctcatcgtcg tctctcccat cttttcctgt gcgcaggcga gccggcctgt taggatgcgc
    69721 tcgcgcgggt gaatcgtcga gcccgcatga tcgcatgaca ctctcacagc catcagccag
    69781 accgcgacaa gccgcggtcc tcttcattct catcacggtc atgctcgacg tcctgtcgtt
    69841 cggaatcatt attcccgtcc tcccgaagct cgtcgaaaac ttcatgtccg gcgacacggc
    69901 gcaggcggcg gcgatctatg gcttcatggg catggcctgg gcgttgatgc agctgctctg
    69961 ctcgccgatc cagggtgcac tctccgaccg gttcgggcga agaccggtcg tgctgctctc
    70021 caacttcggc ctcggcctcg attacatcct catggcgctc gcgccggaca tcacctggct
    70081 ctttgcgggc cgggtcattt cgggtatcgc ctcgtcgagc ttcagcactg ctggcgccta
    70141 tatcgccgac gtcaccccgc cggaggaacg ggccgccggt ttcggcatga tcggcgcagc
    70201 gttcggctta gggtttgtgc tcggtccggc ggtaggcgga ctgttagggg cgagcgatcc
    70261 gcacctgccg ttctggggcg cagccgcgac gagtctcgtg aacgcctgtt acggttactt
    70321 tgtcctgccg gaatcgcttc cgcgcgagaa acggatgaac ttttcctgga agcgcgcgaa
    70381 cgcggtcggt tcgctcgtgc tgctgcgctc gcatcatgaa ctgttcggcc tctctgccgt
    70441 ggcgtttctc aactatctcg cccatgcggt gttacccagc accgcggtgc tctatgtcgg
    70501 ctatcgctac ggatggggat cgacggccgt cggcttcatg ctcgccgctg ccggcatcaa
    70561 cgccatgatc gtgcagggac tgttgatgaa accgctcacc gcgcggttcg gggaacgaaa
    70621 gaccttgctc gcgggcttgt gctgcggggc gatcgggttt tgcgtctacg gcgtcgccac
    70681 cgacggatgg atctattgcg ccggcattcc catcatggcc ttctgggggc tcgccggtcc
    70741 gtcggcgcag gggctcatga ccaaccgcgt gagtgcctct gaacaagggc aactccaagg
    70801 agccatcgcg gggttgtccg gcatcgccgg tctgatcggg cccggtctct tcacgcaaac
    70861 atttgcgctg ttcatcgggc cccgcgccgg ttggcatctc ccgggcgcgc cgtttctcct
    70921 tgcgtcattc ttattactgc tgggaatggc gttagcctgg cgggcgacga ggggagggca
    70981 tgagaagacg ggatgaattg ctccgcgggc ttccgcaagg atattgagcc tgcacgcaag
    71041 gcgtgatcgc tgatcgtggt cagcgtggac tgacgagcct gggctgtttg ccgcctgatg
    71101 ccagttgggc gaactgctgc ttcatcgcat tgacccggat tagataggcg cgcacgtcgt
    71161 ggtcgccgca ccgctggtgg gggctcagcc tgaagcggcg cgcggcatag gcctggccgg
    71221 ccccggcacc gcaaaggtga atgaccgcgg ccagatcctg tttgttccgg agcgtgacag
    71281 tgccgctccg tctctgtccg accgcactca caacttggcg atcgagcagt gccgaagtca
    71341 attcgatggc atggctcggg aggacgcgct ggtagaggtt gttgaaccag caggattgca
    71401 tgtcgtgcca tgggccgtcc tcgaccacga catggtcgtg gatgcagtag cgtttggctt
    71461 cctgaaaggt accatcggta atctggtaca tgccgaccgc actcgacgcc ggtcggtacc
    71521 actccaaagg attccacgac ggctgccatc tccagtaagt tcgcgccacc gggtttcccg
    71581 cgccctcgac ctgcgccagc gccgccaata actcaggcgt gatgaccgct gtcgcatgtt
    71641 cgcggaagag caatccatat tctttccacg tttcggccgg gctcttgttc agtgtacggt
    71701 ccaagggaaa gaggatttcg ctcggcttgt agtacgcgtg ataggtccag ttgacgccga
    71761 gccagaacag gaggatcgcc gataaaatca cgacggcacg caccgacggc ggcgaggcca
    71821 gcacggcctt cagaattcta cgggatgttt tccagcgggc gcgcaggtaa cgccagaagg
    71881 ttttgagggt gcggcgacgg cggcgggagg gacggcgacg ggaggagcgg aggttagggc
    71941 gagaggacat ggggacaata tatatgaaaa tggtgggagg gtggcctgat cggtccctgt
    72001 gctcgccgaa cgcgacacat tgagaaggaa ggaggtccgt tggtcgcctg tgctcgcgca
    72061 acgcggcacg cgggatctat cgaattatca cgtaaagggc ggtgctcgtt ggacgcgcgc
    72121 actgggagac caatcggccc accttccgag aagagagggc aagcgagctt ggaaggacca
    72181 ttgtatattt cacgcgcgca caatggaacc gattcaggcc acctcgaagc ccgagtcccc
    72241 atgagggtga aagagggaaa cgagcaagct tggggggacc attttattta atagtggggc
    72301 cctcccgctg cgggcgcttg ggaaagaccg cgtctcggcg cagtagggat tgggcgggtg
    72361 agaaagtcgc tcgggtcggc gccacccgtg agaatgagga ttcgcaactc agggggtggg
    72421 ggaggaacga cgagcgagct tgggaggaac acgaggatgg tgagcctgct cgctccggcg
    72481 agtggttaag ccgcatggcc ctatcgctgc ttggctgcgc aacttgttcc gtcctggact
    72541 tcgttcgaac ggaacttcgg acaatgctcg ccatcgcttt cagcgaccgc atcgactcga
    72601 ccatgcaacc cctcgcaagt cgcagccagc cccaccatcc tcagtaggcg aatagggaat
    72661 gtcacgcgca caatgggacc gattcaaatc tcaaccttga gaggagaaga aagaatccgc
    72721 cggtggaagc tctgccggct accatctgca gtggaagaag ccggcgcgac tgccgaatgg
    72781 atgatcctat ggtcgtctta gcggaaagat ttgcgtcggc tcacctggcg acgcgcatct
    72841 cggtttacgg ctttgtctcg aataaatgca ccgatgagca gggcggggat tgaaagaagc
    72901 cagagatacc atggggccca caatgtcgcg tcgtgcatga tcattctcct ttcgtgatac
    72961 cagtcgacta cgcaatcacc gtaccaacgt gagaacacgc cccattcccc gtttattcac
    73021 cttgatatgc gattaggaca acgatccgga gcccggatct tgtggtgggc gatgaggcac
    73081 acaacgcaca acggcgttgc acaaacaaca cggatcatga cgatccacca cactgcctcc
    73141 actgcggtgc atttttctgc agtgcattcg ttcacaggct tcaaccggac cggttgactc
    73201 catgggggaa agctgagtag cgtggcattg cctacagggg actcgtccca tccaacgact
    73261 agagtttccc atgccacaga atcgctgcgc catgatcgtc gtggtcggat tgcttgtact
    73321 caccgcctcc tggtcggccg cgcagcagcc tgcctcacaa gcagaatcga gtctcgaacc
    73381 ggagttccgg caactgcgtt cgaaactcag cgccatgccg gaattcagcg ggaccgatgc
    73441 cgaatcaaag tttcgtctgg ctgaagaact cgcccatcgc ggagacgtgc agggtgccgt
    73501 ggaaacctat cgcgccgcga ttcatctcaa acccgactgg ccggatccct accggggact
    73561 cggtcaggtg ctactcgacc atcacgacta tgccgaggcg atccaggcgt tgcagaccag
    73621 cattcgctta ggtcgcgacg atcatcacgc tttctattgg ctgggacgcg cctatatggg
    73681 gaagcacgat ctggctgcgg cggcagtcgc cctcgagcgc gcgacggaac tcaagcccga
    73741 cgatgccgaa acttttgcgg acctcggcct tgttcgaatg acgcagggcg atgatgtcgc
    73801 cgcggaacag gcgctcaagc ggtccattca gttgaagccg gattacgccg aggcccacca
    73861 gctgcgcgaa caactggcca aggccaaagg caacagggag acagtcaaag aagcgggcat
    73921 ggcgcgactc aggagtgtat ttgatcggga ataaattagg gacgccagtc cggctgggca
    73981 ttggaggtcg ataatacgat cgcatgtatg gccaactgat ggccgtccgg ctgttcccag
    74041 gtcttgagca ggagttgatg catctcccgc acctcgatgg cgccgagcga gacccaaccg
    74101 ggtgtggacg gcagttcccc cttcacacaa gtcttcgcgc agagattgat ccagggatgg
    74161 ccgggatctc cgctgccata ggcccatgcc gcataggctc ccgcaggcaa ctgtgaggtc
    74221 gtccgaatcg gcgagacgcc cggaagaggc tggcgaaaca tcaccacacg atccgcgttc
    74281 tcgcgcggca caccgcaacg gtcccccttc gcatagaccc acaacgtgta ttcgccggaa
    74341 ttggccttgc agccttcgaa ggctacgtcc gttccaccct tccaatcgga agcgcgaaag
    74401 atttgcgtgg agggaggtgc tggcacatca gcggcgagaa gtggtgaggg ccagaatgaa
    74461 agcacaaggg cgaggtatgt cgtgacaaga gtcagagtgg cggtgcggaa gtgaaaggaa
    74521 ggcatgaaga gttgtagcga agtagagaag aaaaggtcaa tgcggggagt gtggcgagtg
    74581 aggtgggagg gtggcctgat cggtccctgt gctcgcggaa cgcgacacac gggaacaaac
    74641 agattgataa attattggcg gtgttcgttc gacgcgcgca cgatgggacc gattcaggcc
    74701 actttgaagc ccgtctccgc atgaggggga gagataacga gcaagcttgg aaggaccatt
    74761 ttatagtgga tcaattccgc tgcgggtgct tggtgaaaac cgcgtcccgg cgcgggtggg
    74821 ctgggtgggt gagaaagccg cccgagtcgg cgccacccgc taagagagag tcaacccttg
    74881 cccaggggga ggagcggaac gagcaagctt ggagggatca tcaaaagttc gacgcgcgca
    74941 cgtttgaggc caatcgggcc accccacgcc atggaggaga tgctctagtg aacgaagttg
    75001 gattcccggt gagcgctatc cgtgaagaaa gtcacaacac ccgtatccga taactggcga
    75061 cattgaaatg cacgtatttc ctcactcccc caagctatct ctcttgcgcg atctctctcg
    75121 tgctcttaca attcgctccg tgatactaaa aaatcttctt cagcccggat cggtgttagg
    75181 tttcggggca gtgaaacttc gcatcagcgt atgggtgctg tgcctgctcg tggtgatcgg
    75241 aggatgccag attctgccag gccactggcg gccagggtgg aatcaacgat tgcccgccgg
    75301 cgcgcccgtg cctgacacag ccggctcgtg gaaattgacc gaactcgaac agaaggatgg
    75361 tagatttgtc gccctcgcca tctcaggcgg cgggagtcga gcggccaact tcggtgccgc
    75421 cgtcatgctg gaactccaac agcggggtct gttggaacaa gtcgatgtga tttcaggcgt
    75481 gtccggtggg acacttccgg ccgtgtacta tggactcggg gacaaggccg gtgcgtttac
    75541 ggagccggcg gtgcgccagg cgttgggcta cgattttcag agcagttgga ttcgccggtg
    75601 gtttctccct cagaacatct ttcgctattg gctctccgac ttcacccgtt cggatattat
    75661 ggtgcaggtc ttcaacaacc gtctttacca caaggctacc tttgcagagc ttcggttgca
    75721 tcccaaaatt ctattgaatt ccaccgtcca taacgaccat acccgcttca ccttcacgga
    75781 cgaacgtttc gcagcgctcc attctgtgct cgccaggtat cacgtggcca acgcggtgaa
    75841 tgcgtcgtcc gcctttcccg gtgctttcca ggacgtgacc ctgcaagggt acgtggaacc
    75901 gccgctgtac cttcatctct acgacggcgg tcccatcgat aatcttggcg tccaggccat
    75961 cctcgaatac gtgaatcgga acatcttggg cacctcgctc gatcgtctgt ttccgaacgg
    76021 ctgtctgatt atcgtcgtgg atgccacgcc tgcgagtgag catcctgaac ttagcacgca
    76081 tgagtctagt cgcaaattca tcgactattt cgttgacgac aatgccctgg acgcgatgga
    76141 tgcgatgttg atggaatctc gcagaaccct cttggcgagg atgggcattt cggtcgagaa
    76201 gcaggaccag gatatgcggg gtcggctccc ggtgaatgat cccggtcaat gcggctgcga
    76261 agtacgccat gtcgcacttc gccatctgat gtatgcgggg aactcaggtg atacgggatt
    76321 ggccgagcgc gtgacgcaga tcaaaacgaa gttttgggtt tccgaggagg aacagaacga
    76381 tctctttcaa tccgccaaga tcatcatgaa gctgctagac gatcaaaaac tcctgtcgga
    76441 cccctcattt aaggttccat gcacagttgt cccaccgtcc aagcagtgag ggcgggtccc
    76501 atcgccaacg ccgctcattc atccttgtcc acgcatcccc aatgggtccc tttcactcga
    76561 ggaaatctac tccattatga tcggtcaggg gaaagttttg ctcgcagatt tgtctcagtt
    76621 ggctcgcatc gaccggcttc aagatgtatt gcctggctcc caggcttagc gcctcttcac
    76681 gtatcacgcg atcttgctca gcggtcatca tcacgatcgg cacatgcgga tgctgaaccc
    76741 gtagctccct gagcacttca atgccgtcca tgatgggcat ttgcaggtcc agcagcacac
    76801 ctctgatcgg agactgcttc gcctcaaggg ccatgagcgc caaccctgtc cttccgtcgt
    76861 gagccacgat gacggtatag cccatggcct caagacgatc ttttaaggct tcacagatca
    76921 tgggattgtc atcgatgatc agaagccgtc gtcccatatg acgctgtctc cagaaccgga
    76981 gccggattcg ccgcctccag ggtatatgtg aaaccctctc gccaaagaat gatctccccg
    77041 gtgcgactca gcacgtccat ggcaagaaag agctgactcc aactgaggtc cggcatctgt
    77101 tgcacaattt cctcaaccgt caattttcct gatgcacgaa ggagatccat gatcgcggat
    77161 tcccgttgtg catactcgac gtggtgattc ccggggcggg cctcgctcgg catgggatcc
    77221 tcctttcttc tctcagacgc tcggatcgga ggtgaaacgg ttatcgatca acgtgcgcgc
    77281 tcacggtctg cgtcctgtag ctgcggcgat tcgccaggat gacgggatcg atgatcccgc
    77341 cacattgcac gcaactccat gcgtgggctg tttgtgtccc tgtaatcctg ccccattctc
    77401 catacctgac cggaatgagg aacccctgac agcgcgggca gtgagcaggc cgcacagccg
    77461 gttcactctc cgtcggtggc attgaggccg gctgttcgtt cagcaccatc gttcacctgg
    77521 ttggttccgg ggccgagatc atcctgtcgc cccgcgcgag cggaaccagg acgacgagcg
    77581 tgccccgcat gacgggatgc aactcgcagt gatagccgta gcgaccaggc ggcagggggg
    77641 cgatgaggaa actgctatcc ggcgggaccg cgatggattg aaaagcgcag tggtcatccg
    77701 tcagacatcc atcgtggcgg acgctatgag gagatcccgt tgcattgttc caccgaacag
    77761 gagtgcctgc cgacacgacc gcaacctgag ggtggtagta gggtgacgtc gtttcgaacg
    77821 cgatctcgac gggatcgggt gaggcaaagg cagctactgc gctgccggtc aaaagcatgc
    77881 acgaacagag cagaacgaag acaaccggga atcgattgtt ggatggattg gattggctca
    77941 tggtgccgct cctttccgcg agagggccgt ctcgatctat cgcgccgaca gcatacagcc
    78001 agagggaata aggactcgat atggagatgg gtggaatcgc ataaagaatg tataaagatc
    78061 agccgcgggg gggcacacga attggagaga gaagggatgg ggaattggat gcggaacggc
    78121 gcctaggcag ccgatggggc attgccttcc agactgaagc gatacccgac accggaatcg
    78181 gtcaggatga accttgggcg agtgggatcc ttttcgagtt ttttcctcag ttgcccgatg
    78241 aacacccgca gatactgggc ctggtccaca taggcctgtc cccaaacctc ctgcagcagc
    78301 atgcgatgcg tgagcacttt tccggcatgt tcgatcatgt atttgagcag gtcatactca
    78361 gtcggcgtca gtttgaccgg cttctgatcc agcgtgacct cgcgccggtc aaaattcacg
    78421 cagaggccgc ccgcgatgaa caccggttgc gaactatgag caccggcggc tctccgcaac
    78481 aaggagcgga tgcgggccag caattcatcc atgccgaagg gttttgtcat gtaatcgtcc
    78541 gctccgagtt cgagggcttc gactttgcgg gcctcctcgc ccatcgccga gagcacgatg
    78601 atcggcaccg tggaggattc gcgaatccgg cgcaccactt ccactccgtc tatccccggc
    78661 atgataagat ccaggacaac cacgtcggga cgccggttga gaaaggaacg gagcgcgcct
    78721 tccccggtct ccaccgtctc gactgcatag ccgctttgct cgagattcac ctgaagcgac
    78781 cgccggatct ggggttcatc gtccacgacg agaatgcgcg cgccctgtgt cacgacgccc
    78841 cctgcacagt ggcgggttcg gatgcactcc ggtcactggc gagtaacgag gcggccagcg
    78901 gaatctgaaa caggatcgtg gtctcgtgac cgggaaccga ctcagcgaag atggaaccgc
    78961 catgggcctc gacgatgccc ttgcagatcg ccaacccgag accggtgccg gggctgccgg
    79021 acgacccgcc agattgcacg cgatagaatc gctcgaagat ccggtccagc tcgccgggag
    79081 gcactccttc gccggtattg gagatgcgta cttcgaggaa gttttctgcg agcgccgcgg
    79141 tgagccggat cggggattct ggtggcgaaa acttgatggc gttgtcgaga agatttacga
    79201 gcacctgttg gatttgcacc ccgtcgacag agaccggcgg aagatccgga tccaccagca
    79261 cgtaaagagg ccggccggcc agcaacggct cgacccgtcg gatcgcccct tccaccagtt
    79321 catcgacgat ctgccactcc aggtgaggcg tcagcgtccc ggcctcgagg cgtgacatgt
    79381 ccagcagatt gccgaccaga ccgttcaaat aatccagttc ccgttcaatg cctgtaagaa
    79441 attcgtggcg tacggctggc ggcagcgacg cgccgttgcg gcgaaggctg aacagcatcg
    79501 ttttgatcgt ggtcaacggc gtgcggagtt catgagagac ggaagacaac aacgaggact
    79561 tgatcgcgtc acgctgatgc agagcctgga cggtcatcgc ctggctctgt ttcaacgcct
    79621 gctccgcccg cagaaaccgc caggtgccca acgtcgccac cgccaccacc atgagcacca
    79681 ccacaacttc ccagccaatg agcgtctgga cgttctgttc cgaatgttgg gtcaagagcg
    79741 tcacggcgtc attcgcaatg gtcaggagtg cagcatggtc cctgagcagt gcatccctcg
    79801 caacgccgtt ctgggtaccg gtctgtgcag tctggaggaa cgcatcggcg ttggtaaaca
    79861 atgcctccat gagggtccac tgttcgagca gtttcactcg aatctcgtcg gttggagcgg
    79921 cgggaaccac cgcagttcca ctacgaccca cctggacaac agtcgtgccc ccgtggatca
    79981 acgcctgcac ccgctctttc agcgcggcgc gagtgacctt gtactgcgca tccacaccct
    80041 gggaggccag cagcacctct tttatatgac gctccagcaa catgcgttgt cggcccgcca
    80101 ggtcaatgac gagggcatcg gactcgcggc tgtgaatgat ggtcagggtg agtggaacga
    80161 tggcgagtgc agtgatgatc aggaggccga tgatgcccac gacggtccag ttgacatcgt
    80221 gcgacttcac agcaggtgga gacgcggcgg cagccatagc gacctccagt cgtccccatc
    80281 atatcaatga gacgatggga aactccagga gggagaataa aaggctggcg gcaattgctc
    80341 ctgtaagaac ggtgagggaa aagagggaga ggattcgctc gaacgtctag cggcccccat
    80401 gtccgccacc gccacgacca ccgccaccgc gaccgcctcc accggaatga cctcctccac
    80461 gcccgccaaa atgttgggga ctcgtggacg ggctttggcg tggtaccgag aagggcgcgc
    80521 gaagcacagg aggattcggt tgggcgacat ggggtggatg gacctgttgc agtgtgggtg
    80581 gcagagggcg tgagaactgt tgcggcggcg ccggcgtccg taaggcattg gcaccggttc
    80641 cggcattggc cgatcccggc aacagagtct gtggaggtgc tggtgccggc gtccgtaatg
    80701 cattggcact cgtcgcggca tttgccgatc ccgggagcag agtctgcggt ggcggtgcag
    80761 tgacgcgccc agaggctggc ggcgagacca gcaaagaaga atgcggcgat gcctgcacgg
    80821 aaactcccgg cgatgagggt ggcatggtgc cggcctgtgt cgtgacgacc tgttgcagaa
    80881 ccgggagatg acgattccgc ggatacaggt gttgtgcctg aagcagcaat ccgcggactt
    80941 gttgcacacc gatccaagga taggggtaca cgaccggcgt cacatagacc caatcggtcc
    81001 aggcttggga cacgagggcg ttcgtttgaa gttggtttaa caggtcctga cgttcctgac
    81061 tcagcgcttg aacctgctcc ggcgacacgg cggcagccaa cgcctggttg gccgcatcga
    81121 gttgttggtg caactggtcc gtctctgccc ggagcgccac gagctcgttc ttggcgctct
    81181 cgttttcccg aagcgcggcg atggcctgga caacttcgtc gggatcgact tgcgccgtga
    81241 gatcggcgcg gatcacgacc gtttgttcct ccagccacgt cgtgatcgtc tgatcgagca
    81301 ccacgacgat gccggccgta taggtccgga tgtcatctct cgtgacatcc atatctttca
    81361 cttcggtcac gctctccaaa taggtggcga cctgttcgag tgcatcccgt ttggcggctt
    81421 cgacggccaa tctcacggca tcggcgcgag tgtcgtgatc tcccatgcgg tactcgccgt
    81481 gcgcggtgac cactcgaacg ttggcggaag gagcgcgttg ttcagccggc gactcgagag
    81541 gaggggatgt cgaagaggat tgggattcaa gcgggacaac ccgttgttga tatttctccg
    81601 ggatcgagtc caaatctgtg gtgaagttct gtgtgccgga atcgtctgtg tacttgtaga
    81661 tggtcgcggt cgcgaagaca ttggcagtgg gaacagagag ccacaggaaa catggaataa
    81721 ataccgtaag cgctctgcgg gagattaaca tcacatcccc ctgatctatt gttacgtaca
    81781 gcttaccata tgagtgcccg gttcggagcc ccgtggcctg gggagcgagt agaagctaag
    81841 cagggcctgt catgaagaac agcctcaccg cgcgcactcc acccatgccc gggcgggact
    81901 ctttcgttac acacgtttgg taaaaaaccg cgatctgggt aattgattgt gaagcaatcg
    81961 atggggaagt cggagggaaa cattcggtca ggggtcaggc gggtaagaaa ggctgcgcgc
    82021 agtgggagac cattcaggcc accttcctgg ggagaaaaac gagcgagcgt cgcaagagca
    82081 gcatctaagg aatggcaatc ccgctgcaga aacctcggtc gcgtctatgc tagcatagac
    82141 tacttgttgt cagaccgccg gtttggccgg acccggcgaa gggtatacct gcaggccgtc
    82201 gtatgtcctt cacgaaggtc acaatcatgc cgtcgattcc tgagccgctc aaacaccgtc
    82261 ttgttcagct tcgacgagtg cttcatcagt atccagaact gagcggacag gaaacgaata
    82321 ccgccgccgt catctgcaaa gttctcgatg ggctggccat caaatatcgg gcccacgtcg
    82381 ccggtcacgg agtcgtagcc gacattcccg gacgcgccgg cgtcccctgc gtcgtcttgc
    82441 gtgccgatac ggacgcactg ccgatccagg aagacaccgg cttagaattc gcgtcggtcc
    82501 acgacggcat catgcacgcc tgcggccacg acgggcacac cgcaatgttg cttggcgccg
    82561 cggcactcct ctcagaagag aaagacttac cggctcccgt gcgattgatt tttcagccgg
    82621 cagaagaaaa agggacgggc gcgctggcca tgattaaggc aggggcgctg gaggccgccg
    82681 gcatgatttt cggcggccat ctcgatcggc actaccatcc gggggccatc gttgtggctg
    82741 aaggcgccgt caacgcatcg tcggataatt tcacgattga gattatcggg caaggcgcgc
    82801 acggagcccg tccgcacgaa agcatcgatg ccgtcgtggt cgggagtctg atgatcatgg
    82861 ccttgcaaac gatcgtctca cgcgaaatcg atccggcccg tccgtcggtg gtgtccgtgg
    82921 ggcagttcca cgcagggacc gcgccgaacg tcatcgcagg acaggcgagg ctggaaggga
    82981 cggttcgtgc gcaggatcct gctgtccggc aacaactcat cgcctccatc cgccgcatcg
    83041 ccgaatccat tgcccaattg cacggtgcca agatccaggt gaccgtgaaa gagggcacgc
    83101 cgccgctcat caacacgcct gagatggcga gccttgcgcg ccgcgcggcg gtcacagcgg
    83161 tgggggaggg caatgtattg ccgctcaaga cggccaacat gggcgcggag gatttcagct
    83221 actacatgga aaaaattccc ggggcctatg tccggttcgg cagccaggtc ccgggtaaag
    83281 aaggatttcc cgcccactcc agcaagtttg attttgacga agaggctttg gccgtcggcg
    83341 cggtctatta ccacgccatt gccaaaatcg ccggccagcg actcagcgag cacaagtcgc
    83401 gcacgtgacc atgcacagag ggctgtcatg attcggatca gagaagcccg cgaagaagat
    83461 gtcggccaga tccgcgagat ctttcttgcc gtgtacggcg ccgactatcc ccatcacgag
    83521 gtgtacgacg aactctggct gaagcgctca gtcttcacgg acgacgcgct gattctggtt
    83581 gccgaagaga ctgacacggg cagagtgatc ggcacggctt ccgtgctctt cgacttcggg
    83641 gcccattccg atcttgtcgg agagttcggc cgtcttgccg tccatccgga ctatcgccgg
    83701 ttgcacgtcg gcaggctgct tatggacaag cggctcgaag ccattcagaa ccgcctgcac
    83761 gtcggcctgg tagtcgcgcg caccgtacac ccctatgccc agcgcatcag cctcgcgcag
    83821 ggcttcatcg cggccggctt cctgcccttg aagcatgtct tccaccatcg cgagagcttt
    83881 gccctgctgg ctcggtattt cggcgatgcg ctggccttgc gccgcaacaa cccgcggatc
    83941 attcccgagg cctatgcctt ggccaatctc gtgatgagcc agccgccgct cacgccggat
    84001 ttcatcgtcg acgaagattc cgcgccgtac cccactgggg gggattacac catcgaacag
    84061 ctgcaggctg aaggctatcc ggccttgctg cgcatcgagc ggggccgggt gcgcaacagg
    84121 gaaatcttcg gccccatgcg gctagattac ggattcttca aactccaggc gcggcgaacc
    84181 agttactttc tggcccgctc cggcggccag atcgttgggg ctgtcggcta cacgatggat
    84241 cccgtcgagc acaccgtccg cgtgttcgag ttgatcgccc tggccgacga cgtcgtgcga
    84301 tttcttctcg ctgaattaga actgaagtgc cggcaggaga tggggatcga atacatcgag
    84361 atcgacgtca gcgcctatgc gccgcggatg caacgcacac tcctggaact aaattttctc
    84421 ccggtcgcct atgtgccggc catggtcttc tatcaagtgg agcggctcga catcgtgaag
    84481 atggtgcgat tgaacaagct gcaagatctg gggccgcttg cgctgacgga accggtgcag
    84541 gccgtcgccg acttagtcat gcgcggcttt tcgagctgcg tgattgcgcc gcgcatggca
    84601 caggcgatta aggagattcc attgtttcag gggatgaaca gcgagcaggc gatcaggctt
    84661 gccggagcct gcacggtgcg ggaagtgcgg gcgggcgagc gccttttcgc ggaacacgat
    84721 ccgggggatc ggctctatat cctgctgcaa ggacaagtgg ccattacgtg cggtagccct
    84781 cctgtcagga tcggtactgt ccgcaccggg gaaacttgtg gcgaagtatc gctcctctcc
    84841 gcccgccccc actctgccac ggccacggcc gagggtcacg ttgaggtggc cgaactgctt
    84901 caacgcgatc tggcggacct cattcgccgc cgccccgata tcggcgtcat catctaccgc
    84961 aacctggcgg tcggcctggg cgacaagctc ctgcgctccg ggaattcacg gcgggatcaa
    85021 gggctggctg agtcggaagt cctgcacctg acatcagaac ccgcttcagc cagaaagtaa
    85081 gacccgggtc aactcacgtc catgcatcgt ccttgacggc ggcgagaatg tgtgggagta
    85141 gggcgattgg gtcacggaag ccatgatcta acagtgtaac caaaaagccc gcttattcct
    85201 cctgaaactt cagcgtcttc ccaccttgtc tcacatcaat gctgctgttt cacagcatga
    85261 aggtgttgac actctcacca acgagtcgta gcctttccag aatttcccac tgcaaccggc
    85321 tgaacaggaa aatgtcgctt ttccccgccc tcacgtctga gcacttgaat gcccagcgta
    85381 ctgctggtgc acggagggtc atgacgagca ttctgctcgg gggactcttg accgttccgt
    85441 acgcgatgac ggctctggca gggaatgaat ttacgcaggc atcggtcggg aagggcgtgc
    85501 tcttagtagc cagccccacg ctggatgatc cgaactttcg caaatccgtc gtgctcgtcc
    85561 tggagcacgg gtctgaggga acactcgggg tgattatgaa ttgttccacg aatgtcctcc
    85621 tgtcggaggc gtcgccggac ctcgccgtgc tcaagggaac cagctaccga ttgtttactg
    85681 gtgggccggt ggagccaacc cgcctgatcc tgttgttccg actcaaagag ccaccagccg
    85741 acgcacgatc ggtttttgac ggggtctatg tggggggcac gcctgcagtc ctggaacgca
    85801 tcattacaca ggccaaaccg aacgaaacct tccgcgcttt tgccggagtc gccgggtggg
    85861 ctccggggca gctgcaatat gaaaggctcc aggaggcatg ggcgatcctg ccgccggatt
    85921 caatcggcat ctttgacaag gacccagcca cactctggga ggactgcccc cgccgcctcc
    85981 aggcgccaag ggtcatttcc aattagagaa gtgcgcgggt tctgcctctg gccgcccagc
    86041 cttctggaaa agagtgcagc ggtgatggat ctgcaatctg agaaaagctg gcgattggat
    86101 cttggtgcga gacccatcgg ttcatcggtc gtccagtttc gagtatgggc tcctcgtgcg
    86161 aagaccgtct cagtccatgt gtgccatcca agtcgcaatc cgattccgat ggagtcgcgg
    86221 gaatacggtt acttcgaggt cacgatcgag gatattcgag ccggcgaccg ctaccagtat
    86281 atattggacg accaaaaaat ccggcctgat cctgcgtccc gcttccagcc ggacggcgtc
    86341 catcaggctt ctgccgtggt cgatcctggt gcattcgcct ggacggatcg ggaatggaaa
    86401 ggactcccgc tcaaagacct gatcatctat gaacttcata ccgggacatt tacagcgacc
    86461 gggacgttcg aggcgatcat tcctcacctg gactatctca aacatgctgt gggggtcacg
    86521 gcgatcgaac ttatgccggt cgcccaattt cccggcagac gtaattgggg ctatgacggg
    86581 acgtatcttt atgcgcccca ttccacctat ggtgggccgc aaggactgaa gacgctc
//
LOCUS       DATIFR010000135         1125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_664259, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000135 DATIFR010000000
VERSION     DATIFR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664259"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..672)
                     /gene="aprB"
                     /locus_tag="VLD60_08380"
     CDS             complement(208..672)
                     /gene="aprB"
                     /locus_tag="VLD60_08380"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HSB45013.1"
                     /translation="MPTFVRTDKCDGCKGQDKTACMYICPHDLMKLDKDGSETGHAMK
                     AWNQEPEQCWECYSCVKICPQQAIECRHYADVVPLGGSVQPLRGTDSIMWTIKFRNGT
                     IKRFKFPIRTTPEGSIKPYDGKPKADVSKIDAIEFFSANSIHKTDRSQLVSG"
     gene            complement(756..>1125)
                     /locus_tag="VLD60_08385"
     CDS             complement(756..>1125)
                     /locus_tag="VLD60_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45014.1"
                     /translation="YGCSHLIVGRDHAGVGDYYGPFDAQKIFDEIPKDALETKNMNID
                     WTFWCKKCGGMASQRTCPHTKEDRILLSGTKVRAMLSEGQDLPVEFSRPEVAKVLQKY
                     YAGLSAEQNVKVELKGHSAR"
BASE COUNT          205 a          321 c          337 g          262 t
ORIGIN      
        1 gccttggcgg cctcggtcca ctgtccgatt tcgtacgcgg cgccacacgc ggccataccg
       61 ccgccgataa tgaggatgtc gacttcttcc tcgattacct gcggattacc aaaagtacct
      121 tctgccatga ttgttcacct tgtgtaacgg cgacctgccg gggcacgcgg cccctcgcgg
      181 cagattcgcc ggtggttgtt gaaatgttta accgctgacc aactggctgc ggtccgtctt
      241 gtgaatgctg ttagccgaga agaactcgat cgcatcgatc ttcgacacat cggccttggg
      301 cttgccgtca tacggcttga tcgagccttc cggcgtggtg cgaatcggga acttgaagcg
      361 cttgatggtg ccgttgcgga acttgatggt ccacatgatc gagtcggtgc cacgcagcgg
      421 ttgcacggaa ccgccgaggg gaacgacgtc ggcatagtga cggcactcga tggcctgttg
      481 cgggcagatc ttcacgcagg aatagcattc ccagcactgc tcgggttcct ggttccaggc
      541 cttcatcgca tggccggttt cggaaccgtc cttgtcgagt ttcatcaggt cgtgggggca
      601 gatgtacatg caagcggtct tgtcctgacc cttgcagcca tcgcatttgt cggtacgtac
      661 gaatgtaggc atggtgtcac tccatctatc taaaggttgc agttaaaaac ggttaaaaac
      721 agttaaaacg gttaacgaac tcctgcttct gaaatctatc gggccgaatg gcccttcagc
      781 tccaccttga cgttctgttc ggcggagagg ccggcgtaat acttctgcag caccttggcc
      841 acctccggac ggctgaactc gaccggtaga tcctggcctt ccgagagcat ggcgcggacc
      901 ttggtgccgg acagcaggat gcggtcttcc ttggtgtgcg ggcaggtgcg ctgggaggcc
      961 atgccgccac acttcttgca ccaaaaggtc cagtcgatgt tcatgttctt ggtctcgagc
     1021 gcgtccttcg ggatttcgtc gaagatcttc tgcgcgtcga acgggccgta gtagtcgccc
     1081 acgccggcgt ggtcgcggcc gacgatcagg tgcgagcagc cgtag
//
LOCUS       DATIFR010000136        11450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2005739, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000136 DATIFR010000000
VERSION     DATIFR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005739"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1444)
                     /gene="hemL"
                     /locus_tag="VLD60_08390"
     CDS             complement(161..1444)
                     /gene="hemL"
                     /locus_tag="VLD60_08390"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSB45015.1"
                     /translation="MKITRSAKLFAEAQQLIPGGVNSPVRAFQSVGGQPRFIVRAKGS
                     RLVDVDGNTYIDYVLSWGPMILGHAAPPVINAIKQAAGRGTSYGAPTALEIELARQIR
                     AAVPSMEKVRLVSSGTEAVMSAIRVARGFTRRDTILKFEGCYHGHSDYLLAKAGSGLA
                     TLGIPDSLGVPADFAKHTLTAPYNDIRAVQQVVKERGKQLACIILEPIAGNMGVVPPA
                     PDFLQALRRLTLEHDILLIFDEVISGFRVGYAGAQGLYGITPDLTVLGKIIGGGLPVG
                     AYGGRAEIMDLIAPSGPVYQAGTLSGNPLAVTAGLATLKQLRAKGLYKKLDERAAALA
                     EGIGAAAKKANIPLTQTRVGSMLTSFFTEGPVVDWNSAKLSDTKRYGLFFHKMLEQGI
                     YLAPSQFEAAFLSTAHTTADIEKTIRAADRAFKNL"
     gene            1722..1967
                     /locus_tag="VLD60_08395"
     CDS             1722..1967
                     /locus_tag="VLD60_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45016.1"
                     /translation="MRIVCSWCRKEGKAEFVGEKAPLDDPRETHGICVVHRAEVHAHW
                     WASSQVAADSGDDTRKKVAFALFRWGALLNLTKRIRS"
     gene            complement(2089..2640)
                     /locus_tag="VLD60_08400"
     CDS             complement(2089..2640)
                     /locus_tag="VLD60_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HSB45017.1"
                     /translation="MAARAPLPSLTTHLQSVKAPWSSLLVLKVGQRAESLVDVPDGFV
                     SKVLRGSKCRTSAGLFAEFARALDFPDYFGHNWDALEECLADLEWLPAKGYVLILTDA
                     EQVLQDDEADYATLLEVLSDAGEAWGSGQAGMGSRRATPFHVVFAVGEREKSKRGHWS
                     LQELPAKSPRKPAKPTNKRTGRR"
     gene            complement(2647..3153)
                     /locus_tag="VLD60_08405"
     CDS             complement(2647..3153)
                     /locus_tag="VLD60_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HSB45018.1"
                     /translation="MLGFSPQHDRISPSLFSPARWIAMPHRWMIGFCVLCCLWTGISF
                     GSHDDGASTHSSDPAAEDSQVAAPASEKHRSSGSLDRAEQIAPKKAHDLLATLQQRNG
                     QPLPGYVGGRPFQNREGRLPRGRYREYDVNPKISGRARDAERIVIEQNTGKAYYSGDH
                     YHTFIPLN"
     gene            3330..3917
                     /locus_tag="VLD60_08410"
     CDS             3330..3917
                     /locus_tag="VLD60_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45019.1"
                     /translation="MTIMSLSGVSTVRWAFRGLLAALTLCLLSVHGFDAAAQTGNLDH
                     SPVEIVKKYFALDQKGAKLDALSFETLAPFRDWQEEPAWGRIVVIRGFSVPEHYRQWE
                     IIDRLEVVIPVTFEVIGSVYLETAGFVPESGVEEIRVRVKAVYNRWKIIEPVLPPHVG
                     QKRIINFVREAWVKETEPTKRERLAELQDELRKAK"
     gene            3917..4564
                     /locus_tag="VLD60_08415"
     CDS             3917..4564
                     /locus_tag="VLD60_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSB45020.1"
                     /translation="MGKTAVELMIFDLDGTLIESKWDIAASVNLTLAELGLPQRPLEE
                     IFGFVGDGVKKLLRLAVGEESRVSFEDALKVFRGHYLAHCLDRTRFYPGVEDALTHFT
                     DKRKAVATNKSIEYTEVILKGLGSHHFHFVVGGDNGFGLKPEPGMLLHVMERLKVPKD
                     RTVLVGDSTNDINGGHNAGIRVCAVGYGMGNRQKMAACQPDWFIERPEELMEIFE"
     gene            4591..6168
                     /locus_tag="VLD60_08420"
     CDS             4591..6168
                     /locus_tag="VLD60_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB45021.1"
                     /translation="MRRTMRWVAAPCIGILLNLSLSLSLVLASPGLADRVIEHKLANG
                     LTVLMVERHQTPIVSINMTFGVGGVNEQAGQTGLAHLYEHMAFKGTRTIGTKDYTKEK
                     PILDELHRIGTGLEERQRDLAKRSPENPVSTEEQATIEALQKRFKELQDQAVQYVAGN
                     EMALLYQRHGGVGLNAATGKDLTRYMISLPSNRLPLWAAIEADRMANPVLREFYKERG
                     VVMEERRLRNDDSPNGLLFETFTSAAFRAHPYGIPTIGWESDILSLTPAATEAFFKTY
                     YGPGRATIGIVGDINPQEVIALIESTFGKIPAVPPPPQIVTTEPPQRGERRIEIEFDA
                     EPSLAIGFHKPGLGHPDDYVFDVIDEILTEGLTSRLYVKLVRDGRLAASVSSDANYPG
                     VRAPNLFVLNATPAAPHTTADVEAAILAELERLKTEPVTSKELERVLNNLDADMVRAL
                     RSNSGLASQLALYQTVAGDWHYILKARDKVAAVTPADIQRVAAQYFTKSNRTVATLVK
                     KGDEKQVATVAPGEVAR"
     gene            6165..7619
                     /locus_tag="VLD60_08425"
     CDS             6165..7619
                     /locus_tag="VLD60_08425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB45022.1"
                     /translation="MKVVKSLALLSFTGIAALLVLSVVTAWAGDLALGDPRKMTFKPV
                     EFAPPEPDRVVLDNGMVVYLLEDHELPLISLSTTMRTGGWLEPADKIGLAGLTGSVMR
                     TGGGGGLTAEQVDEELEQFAGDISISMGRQAASASLDVLSKDLKRGLQIFAGLIRTPA
                     FEPARVDVAKLQAIEGIRRRQDNPGSIVGREFVKLLYGPEHPSARESTIESIKQITRD
                     DLVSFHQRTVHPNGIILGVTGDFEKAEMLALLRATFGDWKKGDVPELKIADVPESSGA
                     RSIVRFVKKDTSQTHLRVGHLTIKENDPDYVSLAIANDILGGSSFRSRLFNDVRTKRG
                     LAYSVGSRLNAGVHDQGVWLMRAETKLPSTEEVISRFVANMQRMRTELVTDEELAEAK
                     EAYVNSFVFSFTSPAAIVNRFIDLEYDGLPKDFLQQLRARVVALSKEDLLAAAKKHFH
                     PDRLTIVAVGPGEALAKLLSGFGEVKEIKLVPEG"
     gene            7841..8683
                     /locus_tag="VLD60_08430"
     CDS             7841..8683
                     /locus_tag="VLD60_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB45023.1"
                     /translation="MPLEDDLCDMIKKARAGLKLSVGELARLTGLPGADISALERGDQ
                     VRDRTELRAIASALGLRQRPLEEIVLDKWVPHSLPLMPGIETVHGEISGYAVKGYIVY
                     DSGEAVLIDTAYNAETMIEILEARSLRLIGICLTHGHADHAEGIQRILNRWQVPVFLG
                     SDDVTLLHWKPAETQLVHPDDGRMIPIGRLTVRCLAAPGHTPGGICYSVEMPWGPVCF
                     VGDTIFAGSIGRSNPFQLYHAHLESVRHKVLSLPQDCLLLPGHGPGTTVQEEVQHNPF
                     YTTH"
     gene            8694..9683
                     /locus_tag="VLD60_08435"
     CDS             8694..9683
                     /locus_tag="VLD60_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSB45024.1"
                     /translation="MDGTNRGEKQSIRRNDGQAPLPSGQSEHPLNPSFDEVEEDESSF
                     SRWTLPLALFLLTIFTTLWAGAYQAYSGPMRGPVAFLQEHPEMLWRGIPFAGTLLFIL
                     VTHELGHFLLSKIHRVPASLPLFIPGPPHFIGTFGAIIRIRGPIMNRRALFDIGVAGP
                     LAGFVIAVAALVVGLHLSTVVDRTTTYGLQLGEPLLLKFMAWLIIGSLPPEADVLLHP
                     IAFAAWFGLFVTSLNLIPIGQLDGGHVAYALWGSRQRTMAFAIVPLLIVLGFVGWPGW
                     FLWAFMAGLWGFGHPPVMDPHVPLGRGRTVVGWMALAVFVLTFAPVPFSFYSR"
     gene            9754..10506
                     /locus_tag="VLD60_08440"
     CDS             9754..10506
                     /locus_tag="VLD60_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45025.1"
                     /translation="MAPTSITTLACPKCSFEQPDGGIECAKCGIIFAKYRLHPVQPGM
                     QRTGFSLTESPWFLAAKRRFIDLDQTNDPLTFAGRLVVFLALIWWGKSFVVTPLETNA
                     TGESFFHLVNLPFHEAGHLIFMPFGRFMMFLGGSLTQILIPVVCLSTFLVKTRDPFGA
                     SVALWWTAENFMDVSPYINDARALDLMLLGGVTGKETDGHDWENILGMLGWLQYDHRL
                     AHLSNNLGIALMLVSFAWGALLLLRHYRRLSS"
     gene            10575..11045
                     /locus_tag="VLD60_08445"
     CDS             10575..11045
                     /locus_tag="VLD60_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HSB45026.1"
                     /translation="MRLLGMVSLCVILFTPTGLLYAADSSGSSRFTLVLGGAAVKDNQ
                     TGLIWEQEPDREHDVWSRSVARCATKEVGGQKGWRAPTVDELKTLIDSSQHDPALPAG
                     HPFSNIKSEIYWTATPHPTDDIVAWQISFFSGEPVTDQKSGTRRLWCLLDQAKK"
     gene            11059..>11450
                     /locus_tag="VLD60_08450"
     CDS             11059..>11450
                     /locus_tag="VLD60_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSB45027.1"
                     /translation="MIHLLWVCWVLLSLLPAPSLAEEPASTSQLSASFEPPPGWHYGA
                     YLDLSYIQNFNYPENHLWRSRTTTQRNNELTPNMALIYVRKDATESSRWGTELGVQGG
                     YDTDNFAFLIGESKVSGADTLRQIHRANV"
BASE COUNT         2379 a         3242 c         3337 g         2492 t
ORIGIN      
        1 agggggctct ctaccggcct tgcgagactc gatggctgta tcgaaagcga tccgaaggat
       61 atctccctcg atacaaccgc agagaacatt ttgagcgtga ccggagccga agaggacgtt
      121 ggatcttcgt ctcatcaggt catcagctgc ccgctattcg ctataaattc ttgaaggctc
      181 tgtctgcggc gcgaatcgtt ttctcgatgt cggccgtcgt atgggctgtt gagagaaatg
      241 ccgcttcgaa ttgggagggg gcgaggtaga tgccctgttc gagcatcttg tggaagaaca
      301 gcccgtaacg tttcgtatcc gagagttttg cactattcca atccacaacc ggcccttccg
      361 taaagaacga cgtcagcatc gatcccaccc tggtctgcgt cagtggaata tttgccttct
      421 tggccgcagc gccgatgcct tcggcaagag cagcagcccg ttcgtcgagc ttcttgtaca
      481 atccctttgc tcttagttgt ttgagcgtgg ccagtcccgc cgtcactgcc agcggatttc
      541 ccgataacgt accggcctgg tatacaggac ccgatggtgc gatcagatcc atgatctccg
      601 cccgccctcc gtacgcccct accggcaatc ctccgccgat aattttgccc agcacggtca
      661 ggtccggcgt gatgccatag aggccttgtg ctcctgcata gccgacgcga aatccggaaa
      721 tgacttcgtc gaagatgagc aggatgtcgt gctcgagagt caaccggcgg agcgcttgga
      781 gaaaatccgg cgcgggaggg accacgccca tgtttccggc gatcggctcc aaaatgatgc
      841 aagccagctg tttcccgcgt tctttgacga cctgctgaac ggcgcgtatg tcgttgtaag
      901 gagcggtgag ggtatgttta gcaaaatccg ccggcacgcc aagcgaatcg gggattccca
      961 acgtagccag ccctgaaccg gctttggcca gaaggtaatc actgtggccg tggtaacagc
     1021 cctcaaactt gaggatggtg tctcgcctgg tgaatccccg agccacgcga attgcgctca
     1081 tgaccgcttc ggtcccggag ctgaccaggc gaaccttctc catcgaggga acagccgcac
     1141 gaatctggcg ggccagctcg atttctaatg ctgtcggagc tccgtaactg gtccctcgtc
     1201 ccgcagcctg tttgatcgcg ttgatgacgg gaggtgccgc atgtccaagg atcatcggtc
     1261 cccaagacaa gacgtagtcg atgtaggtgt ttccatccac atcaacgagc ctcgaccctt
     1321 tggcgcgaac gatgaaacgg ggctgccctc cgaccgattg gaaggcacgg accggactat
     1381 tcacaccacc gggaatgagt tgttgcgcct ctgcaaagag cttggcagat ctcgtgattt
     1441 tcataacggg ggcgcaacct aacatgcagg ggaagaattg gtcaaggaaa ggaaagaacg
     1501 ccgcgaattg ccccatgtgg ggtcaaaaag tgtctgatga gatactagcg ctggcggttt
     1561 ttggatacac cggttcgatc tctctcgagc actctgctcg aattctcagg aattattgat
     1621 cttgtttcgt ctcccagaat tcggcccgac acttgcgtac aaatttggtc atggctcacg
     1681 attatgatgg ttgaaggaaa gtactatttc gagggtcgaa aatgaggatc gtctgttcct
     1741 ggtgccgaaa agagggaaag gccgaatttg tcggcgagaa ggctccgctg gacgatcctc
     1801 gagaaaccca tggcatctgc gtcgtgcatc gtgcggaggt gcatgcgcat tggtgggcgt
     1861 cgagtcaggt cgccgctgat tctggtgatg acacgagaaa gaaagtggcg tttgcgctct
     1921 ttcgctgggg cgcgctcttg aatctcacaa aacgaatacg ctcttgagaa gcacattctc
     1981 cttgcaactt tgtggtatca cgcctccaat cgcacggaac atctgtagaa accgatcgat
     2041 tccacgatga cttgtcctca cgaatccatg agccttgccg acgcatgttc aacggcgccc
     2101 agttcgctta ttggtgggct tcgccggctt tcgcggtgat ttggcgggca attcttgaag
     2161 gctccagtgc ccgcgctttg atttctcccg ttccccaacg gcgaatacca cgtggaatgg
     2221 agtcgcacgt ctggagccca tgcccgcttg accagatccc caggcctctc cggcatcgct
     2281 tagaacttcc aagagcgtgg cataatccgc ttcgtcgtct tgaagcacct gttccgcgtc
     2341 ggtgagaatc agcacataac ctttcgccgg caaccattcc aaatcagcca ggcattcctc
     2401 gagtgcatcc cagttgtggc cgaaataatc tggaaaatcc aaagcccgcg cgaactccgc
     2461 aaataacccg gcgctcgtac ggcacttgct gccccgcaag acttttgaga cgaaaccgtc
     2521 tgggacgtcg accagcgatt cagctcgttg tccgaccttc agcaccagga gggacgacca
     2581 gggtgccttg accgattgca agtgagtcgt cagcgaaggt agtggtgctc gagcggccat
     2641 agcgtctcaa tttaacggaa tgaaggtatg ataatgatct ccagagtagt aggctttccc
     2701 cgtattctgc tcgatcacaa tccgctccgc atcgcgtgcg cgcccggaaa tcttcggatt
     2761 cacgtcgtat tcccggtaac gaccacgcgg aagccgccct tcccgattct gaaatggacg
     2821 ccctccgacg tagccaggaa gcggctgccc gttgcgctgc tgcagcgttg ccagcaaatc
     2881 atgcgccttt ttcggcgcga tctgctcagc ccgatcgagc gatccggatg agcgatgctt
     2941 ctccgatgcc ggcgccgcga cctgactatc ctcagcggca gggtcagagc tgtgggttga
     3001 tgcgccatca tcgtgtgatc caaatgaaat gcccgtccat aggcagcaga gaacacagaa
     3061 cccgatcatc cagcgatgcg gcatcgcgat ccatcgtgca ggagagaaga ggcttggcga
     3121 aatgcgatcg tgctgcgggg aaaaccctag catgtggtct cgaagtaggt caacgtcgag
     3181 ccatcggtcg atcgcgcgtg catgaaatcg gccacaacct ttgatacaat gcggtgccgg
     3241 tccactgtgt accagttgag gatgttgtcc aagcccgtgc cgcgacacct tccgccggcg
     3301 aatcctttcg gaacaacgat cgattacagg tgacgattat gtccctatcc ggtgtctcga
     3361 ccgtgcgttg ggcgttcagg ggccttctcg ctgccctgac cctctgtctc ttgtctgttc
     3421 atgggttcga tgccgcagct caaacgggca acctcgacca ttcgcccgtt gagatagtga
     3481 agaagtattt tgcccttgat caaaaagggg ctaagctcga tgcgctgtcc ttcgaaacac
     3541 tcgcgccctt cagggattgg caggaagaac cggcgtgggg gcgaatcgtt gtcatccggg
     3601 gattttccgt cccggaacac tatcgccagt gggagatcat tgaccggttg gaagtggtta
     3661 tcccggttac atttgaagtg atcggttcag tttatctgga aacagccggg ttcgttccgg
     3721 aatccggtgt cgaggaaatt cgcgtgcgcg tcaaggcagt atataatcgg tggaagatca
     3781 tcgagccggt gctgccgccc cacgtcgggc agaagcggat catcaatttc gtgcgggaag
     3841 cctgggtgaa ggaaactgaa ccaacgaaac gcgagcgtct ggcggaattg caggacgagt
     3901 tacgaaaggc gaagtgatgg ggaagacggc agtcgaattg atgatcttcg atctggacgg
     3961 caccctgatc gaatccaaat gggatattgc cgcctcggta aatctgacgc tggctgagtt
     4021 ggggttgccc caacggccgc ttgaagaaat tttcggtttc gtcggcgacg gagtaaagaa
     4081 gttgctgcgg ctggcggtag gggaagagag ccgcgtcagc ttcgaggatg ccttgaaagt
     4141 atttcgagga cattacttgg cccattgcct ggatcggacc cggttctatc cgggcgtgga
     4201 agacgcgttg acccacttca cggacaagcg caaggccgtc gcgacaaaca agtcgatcga
     4261 atatacggag gtgatcctca agggattggg ctcccatcat ttccattttg tcgtcggcgg
     4321 cgacaacggg ttcggcttga aaccggagcc aggtatgctg ttgcatgtca tggagcgcct
     4381 gaaggtcccg aaagatcgaa cggtgttggt gggagacagc acgaacgaca tcaacggtgg
     4441 acataacgcc ggaattcgcg tctgcgcggt cggctacggc atgggtaacc ggcaaaagat
     4501 ggccgcttgc cagccggatt ggtttatcga acgccctgaa gaactcatgg agatcttcga
     4561 atgaacaaca ggtcaagctc gacggaccac gtgagacgaa caatgcggtg ggttgccgct
     4621 ccttgtatcg gcattctact caacctgagc cttagcctca gcctggtgct tgcgtcgccg
     4681 ggcttggcgg atcgcgtgat cgagcacaaa ctcgcgaacg ggttgaccgt gttgatggtc
     4741 gaacggcatc aaacgcccat cgtgtcaatc aacatgacgt tcggtgtcgg tggcgtgaac
     4801 gagcaggcag gtcagacagg tctcgctcat ctgtacgaac acatggcatt taaggggaca
     4861 agaacgatcg ggacgaagga ttacaccaaa gagaagccaa ttctcgacga actgcatcga
     4921 atcggaaccg gactcgagga acgccaacgg gacctcgcca agcgcagtcc ggagaatccc
     4981 gtcagtacgg aggagcaggc gacgatcgaa gcgttgcaga agcgattcaa ggaactccaa
     5041 gaccaagccg tccaatatgt cgcggggaac gagatggcgt tgctgtacca gcgtcacggg
     5101 ggcgtcggcc tgaatgccgc cacaggcaaa gacctcacgc gatatatgat cagcctgccc
     5161 tccaatcggc tgcccctctg ggcagcgatc gaagccgacc gtatggcgaa tccggtgctg
     5221 cgggaattct ataaggaacg tggcgtcgtc atggaagagc ggcgcctgcg aaatgacgac
     5281 agccctaacg gcctgctctt cgaaaccttt acgtcagcag ccttccgtgc ccatccctac
     5341 ggcattccaa ccatcgggtg ggaatccgat atcctgtcat tgacgccggc tgcgacggaa
     5401 gccttcttta aaacgtatta tgggcctggc cgggccacca tcgggattgt cggcgacatc
     5461 aacccccaag aagtcatcgc gctcatcgag agcacgttcg gcaagattcc agctgtgccg
     5521 cctccgccgc agattgtcac gacggaacct ccccagcgag gggaacggcg cattgaaatc
     5581 gaattcgatg ccgagccgtc cctcgcgatc ggtttccaca agccgggact cggccatcca
     5641 gacgactatg tgttcgacgt gatcgacgaa attctgacgg aaggcctgac ctcacggttg
     5701 tatgtgaaac tggttcgtga cggccggctg gcggcttcgg tgagttccga tgccaattat
     5761 cccggagtcc gtgcaccgaa tttattcgtc ttgaacgcca cacctgcagc acctcacacg
     5821 actgcggatg tcgaggcggc cattctcgct gaactagagc ggctcaagac cgagcctgtg
     5881 acatcgaaag agctcgaacg ggtgctcaac aaccttgatg ccgacatggt ccgggcgctt
     5941 cgatccaaca gcgggctggc gtcgcaattg gcgctgtatc agaccgtcgc aggagactgg
     6001 cattacattc tcaaggcgag ggacaaggtg gcggcggtca cccctgccga cattcagcgc
     6061 gtggcggcgc aatactttac gaaatcgaat cgaacggtcg cgaccttggt gaagaagggc
     6121 gatgagaaac aggttgcgac agttgctcct ggcgaggtgg cgcgatgaag gtggtgaaga
     6181 gtttggccct gctaagtttt acgggcatcg cggcattgtt ggtcctgtcc gtggtgacag
     6241 cctgggcagg agatcttgcc cttggtgacc cgcggaagat gaccttcaag ccggtagaat
     6301 tcgcgccgcc cgagccggat cgagtcgtcc tcgacaacgg catggtcgtc tatcttttgg
     6361 aagatcacga attaccgctg atcagtctct cgaccacgat gcgcaccgga ggatggctcg
     6421 aaccggccga caagatcggg ctggcggggt tgaccggctc ggtcatgcgg accggcgggg
     6481 gcggcggatt gaccgccgaa caggtcgatg aagagttaga gcagtttgca ggagacatca
     6541 gtatctccat ggggcggcag gccgcgtcgg catctctcga cgtcctgagc aaggacctga
     6601 agcgtgggct gcagatcttt gccggcttga tccggacacc ggcattcgag ccggcgcgcg
     6661 tcgatgtcgc caaactgcaa gccatcgaag ggatcaggcg ccgccaggat aaccccggat
     6721 cgatcgtcgg ccgggagttc gttaagctgc tgtatgggcc ggagcatccc tccgcccgcg
     6781 aaagcaccat tgaatcgatc aaacagatta cccgtgatga tctggtgtcg ttccatcagc
     6841 ggacggttca ccccaatgga attattctag gagtcaccgg cgacttcgag aaagcggaga
     6901 tgctggcgct gttgcgcgcg accttcggcg actggaagaa gggcgatgtg ccggagttga
     6961 agattgccga tgtgccggag tcatccggtg cacgttcaat cgttcgcttt gtcaagaagg
     7021 acacctcgca gacgcacttg cgtgtcggcc acctgaccat caaggaaaat gatcctgact
     7081 atgtttcgtt agccatcgca aacgacattc tcggcggcag ttcgttccgc agccgcctgt
     7141 tcaacgatgt gcgaaccaaa cggggcctgg cctattccgt cggcagtcgg ttgaacgccg
     7201 gtgtgcatga tcaaggtgtc tggctcatgc gggcggaaac aaagctgcct tctacggaag
     7261 aagtcatcag ccgttttgtc gccaacatgc agcggatgcg aaccgaattg gtgaccgacg
     7321 aggaactggc ggaagccaag gaagcctatg tgaactcgtt cgtgttttcc ttcacgagcc
     7381 ctgcggcaat cgtcaatcgc ttcatcgatt tggaatatga cggcctgccc aaggacttcc
     7441 tgcaacagct gcgggcgcgc gtggtggcgt tgtccaaaga ggatcttctg gcagcggcca
     7501 agaagcattt tcatccggac cggctcacga tcgtcgccgt ggggcccggc gaggccttgg
     7561 ccaaattgtt gtcgggattc ggtgaggtga aagaaataaa gttggttccg gaagggtaaa
     7621 gaggagcgag tcctgccgtc gaccagctca ttcgccctcg ctccacccat cccccagtgg
     7681 gtcccttgcg ctgcgggcga atgggctggc cgacgacacc cgcttcaaag aaaagatttt
     7741 ccggaacggt ggggggatag aggggaatcg gatagtcgag ttgaggtctg cattggagcg
     7801 ccaaaaattg tgctagtacc tgtcctctga acgattgcct atgccacttg aagacgactt
     7861 gtgcgacatg atcaagaagg cccgtgcagg cctgaaactg tctgtcggtg agctggctcg
     7921 tctgaccggc cttcccggag ccgacatcag cgcgctggag aggggtgatc aggtgcgaga
     7981 tcgcacagag cttcgcgcaa ttgccagcgc cctcgggttg cggcagaggc cgctcgaaga
     8041 gatcgtgctg gacaagtggg ttcctcattc cctcccgttg atgccgggga tcgaaaccgt
     8101 acatggcgag atcagcggct atgcggtgaa gggctacatc gtctacgatt ccggcgaggc
     8161 ggtcctgata gatacagcct acaatgccga aaccatgatc gagatactgg aggctcgatc
     8221 ccttcgcttg atcggcatct gtcttactca cgggcacgca gatcatgcag aggggattca
     8281 gcgaatcctc aaccggtggc aggtgccagt cttcctcggc tccgacgatg tgacattatt
     8341 gcattggaag cctgcggaga ctcaattggt tcacccggac gacgggcgga tgataccgat
     8401 cggccggttg acggtccgct gtcttgcagc ccccggtcat acgccaggcg ggatttgtta
     8461 ttcggtcgag atgccctggg gaccggtgtg tttcgttggc gatacaatat ttgccggatc
     8521 gatcgggcga tctaatccct ttcagctgta tcacgcacat ctcgaatcgg tgcgacacaa
     8581 agttttaagc ctgccgcagg actgtctgct cttgcccggg catggtccgg gaacgacggt
     8641 acaggaagaa gtccaacaca acccgtttta caccacgcac tgacggcggc cacatggatg
     8701 gcaccaatcg tggcgagaaa caatccattc gcaggaacga cggccaagcg cccctgccct
     8761 ccggtcaatc cgagcaccca ttgaatccct cgttcgatga ggtggaagag gacgagtcat
     8821 ccttctcccg ctggacgttg ccgctcgcgc tctttctcct gacgattttc accacgcttt
     8881 gggccggcgc ctatcaggca tacagcggtc cgatgcgagg gccggtcgca tttctgcagg
     8941 agcatccgga aatgctgtgg cgaggaatcc ccttcgctgg cacgctcctg ttcatcctcg
     9001 tcacgcacga gctcgggcat ttcctcttgt cgaagatcca tcgggttccg gcctcactgc
     9061 cgctgttcat tccggggccg ccgcatttta tcgggacgtt cggcgcaatc attcgcatcc
     9121 gcggcccgat catgaaccgg cgcgcattgt tcgatatcgg cgttgccggg ccactggccg
     9181 gattcgtcat cgccgtcgcg gccctggtcg tcggcctaca cttatcaacc gtcgtcgatc
     9241 gaacgaccac gtacggattg cagctcggcg aaccgctgtt attgaaattc atggcatggt
     9301 tgattatcgg gtccttgccg cccgaagccg acgtgctgct ccatccgatc gccttcgcgg
     9361 cctggttcgg tctctttgtc acgtcgttga atctcattcc gatcggccag ctcgacggcg
     9421 gacatgtcgc ctacgcatta tggggaagcc ggcaacggac catggcgttt gcaatcgttc
     9481 ccttgctgat cgttttggga tttgtcggat ggcccggctg gtttctttgg gccttcatgg
     9541 caggactctg gggtttcggg catccgccgg ttatggatcc tcatgtccca ttggggcgag
     9601 ggagaaccgt cgtcggttgg atggccttag cagtctttgt gctcaccttt gcgccggtcc
     9661 cattctcttt ctactccagg taatcttgcg gtcttacaga caagaatcct tgcaatttct
     9721 tgccctctcg cagcttcctt cgtagtctgg gttatggctc cgacgtcgat taccacgctc
     9781 gcctgcccca agtgttcgtt cgaacaaccg gacgggggta tcgaatgtgc caagtgcggg
     9841 atcatctttg ccaaataccg gctgcatccg gttcaaccag gcatgcaacg gaccggcttc
     9901 tcactcaccg aatcgccttg gtttctcgca gcgaaacggc ggttcatcga tttggatcag
     9961 acgaacgatc ctctgacgtt cgccggacgg cttgtggtct tcctcgcact gatttggtgg
    10021 ggcaagagtt tcgtcgtcac tccgctcgag acgaacgcca ccggcgaatc gttcttccat
    10081 ctggtcaatc ttccgtttca tgaagcgggg catctgatct tcatgccctt cggacggttc
    10141 atgatgtttc tcggagggag cctcacgcag atcctgatac cggtcgtttg tctctcgaca
    10201 tttctcgtca agacccgcga tcccttcggc gcatctgttg cgctctggtg gacggcggaa
    10261 aacttcatgg acgtgtcccc ctacatcaac gatgctcggg cactggatct gatgctgctc
    10321 ggcggcgtga cgggaaaaga aacagatgga cacgactggg aaaatatcct cggcatgctt
    10381 ggttggcttc agtacgacca ccgtcttgcc catctctcga ataatctcgg catcgccttg
    10441 atgctggtat ctttcgcctg gggagctctt ctcctgcttc gccactatcg ccgcttgtcc
    10501 tcgtgagcca ccacacttgc gcgccctttc aaactctctt acgataggcc cccctattac
    10561 tgagaggagc gtccatgcgc ctgttgggaa tggtgagtct ctgtgtcatc ctcttcacgc
    10621 cgaccggctt gctgtatgcc gccgattctt ccggctcgtc gagattcacc ttggtgctgg
    10681 gcggagctgc cgtaaaagat aaccagacag ggctgatttg ggagcaggag ccggatcgcg
    10741 aacatgatgt ctggagtcgc tccgttgccc gatgcgccac aaaagaggtg ggagggcaga
    10801 aaggctggcg tgccccgacg gtggatgaat tgaagacgtt gatcgattcc tcacaacacg
    10861 atcccgccct gccggccgga catcccttct cgaacatcaa gtccgaaatc tattggacgg
    10921 ccacgccgca tcccaccgac gatatcgtgg cctggcagat cagcttcttc agcggcgaac
    10981 cggtgaccga ccagaagtcc ggcacacgac gcttgtggtg cctgttggat caggcaaaga
    11041 aataggttgg cccgccggat gatccatctg ctgtgggtct gctgggtgct cttgtcactc
    11101 ctgcctgctc cgtctttggc cgaggaacct gcatcgacga gccagctctc cgcgtcgttc
    11161 gagccgcctc ccggctggca ctacggcgcc tacctcgatc tcagttatat tcagaatttc
    11221 aattatcccg agaatcacct gtggcgcagc cgcaccacta cacagcgcaa taacgaattg
    11281 acccccaaca tggcgctcat ctatgtcagg aaagacgcga cagaatcctc tcgctggggc
    11341 acggaactcg gtgtgcaggg cggatatgac acggacaact tcgcgtttct catcggtgaa
    11401 tcgaaagttt ccggtgccga tacactgcgt caaattcatc gcgccaatgt
//
LOCUS       DATIFR010000137         1350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_664939, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000137 DATIFR010000000
VERSION     DATIFR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664939"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VLD60_08455"
     CDS             <1..782
                     /locus_tag="VLD60_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSB45028.1"
                     /translation="NDLDHEVSPFLMLDYAGPQYFAPTDHPRGVGEHPHRGFETVTIV
                     YEGVVAHRDSAGNAGVIGPGDVQWMTAASGVVHEEFHEKAFAKKGGRLHAIQLWVNLP
                     KASKMSAPGYQTILNADIPAIDLNDGAGRLRVIAGSFQGRKGPAHTFTPVELYDLELK
                     GGQSIVLTIPDEHNASIFVLQGRASVNGSPAAGEAELIVCKRNGSQVTVEAEKDSRIL
                     VMAGNPIDEPIARYGPFVMNTKAELIQAVQDYQVGKMGHLS"
     gene            779..>1350
                     /locus_tag="VLD60_08460"
     CDS             779..>1350
                     /locus_tag="VLD60_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HSB45029.1"
                     /translation="MSDQTLMIEIYSDVVCPWCYIGKRRLERALSQLKDAVRPQIIWR
                     PFQLNPTMPKEGMDRTTYLEAKFGSLEAFRRLEEHVLEAGAEERIAFAFEKIARTPNT
                     LPAHRLIWYAAQQGCQDAVVDSLFKGYFEEGADIGTTSVLVQLADRVGLNAAHFLQSD
                     GGVAEVKAEESAGHKLGIRGVPYFVLNQVYG"
BASE COUNT          298 a          342 c          422 g          288 t
ORIGIN      
        1 gcaatgatct cgatcatgag gtgagcccct ttctcatgct cgattatgcg ggaccccagt
       61 actttgcccc gacagatcat cctcgcggcg tgggtgagca tccgcaccgc gggtttgaaa
      121 ccgttactat tgtctacgaa ggcgtggtgg cgcatcgcga ttctgctggg aatgccggtg
      181 tcatcggtcc gggcgacgta caatggatga ccgcagcctc tggcgttgtc catgaggagt
      241 ttcacgaaaa ggcatttgca aagaaaggtg gcagactcca cgccattcaa ctgtgggtga
      301 acctcccgaa ggcgtcaaaa atgtccgcgc ccggctatca gacgattctg aacgcggaca
      361 ttccggcgat tgatctcaat gatggagccg gtcggttgcg cgtgatcgcc gggtcgtttc
      421 aggggcgcaa gggcccggct catacattta cgcctgtcga actctatgat cttgagctaa
      481 aaggcggaca gagcatcgtg ttgacaatcc ccgatgaaca caatgcgtcc atctttgtct
      541 tgcagggtcg agcttccgtc aatgggtcgc cagcggccgg ggaagcagag ttgatcgtgt
      601 gtaagaggaa cggctcgcag gtcaccgttg aggctgaaaa ggacagccgg atcttggtga
      661 tggccggaaa cccgatcgac gagccgattg cccgctacgg gccgtttgtc atgaatacca
      721 aggccgagtt gattcaagcg gtacaggact atcaggtagg gaagatgggg catttgtcat
      781 gagcgatcag actctcatga tcgagatcta ttccgatgtc gtctgtccct ggtgctacat
      841 cgggaagcga cggttggaac gagcgttgag tcaattgaag gatgcggtaa ggccgcagat
      901 aatctggcga ccgttccaac tgaatccgac gatgccgaag gaagggatgg atcgaaccac
      961 gtatctcgag gcaaagttcg gaagtctcga ggccttccgg cgattggaag agcatgtgct
     1021 ggaggctggt gccgaggaac ggatcgcctt cgcgtttgag aagatcgcgc ggacgcccaa
     1081 tacgttgccg gcccatcggc tgatttggta cgccgcgcaa caaggctgcc aggatgccgt
     1141 ggtcgattca ctcttcaaag gttacttcga agagggcgct gatatcggaa cgacatccgt
     1201 gcttgttcaa ttggccgacc gtgtgggact gaacgcagca cactttctcc agagtgatgg
     1261 cggagtggcg gaggtgaagg ccgaagaatc agccggacac aagctcggga ttcgcggcgt
     1321 cccctatttc gtgctgaacc aagtctatgg
//
LOCUS       DATIFR010000138         5936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1431642, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000138 DATIFR010000000
VERSION     DATIFR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431642"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VLD60_08465"
     CDS             <1..1279
                     /locus_tag="VLD60_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSB45030.1"
                     /translation="IAILLGLIVLSAVISTAEIGFFAVNETKLRALAQNNNNRAKMAL
                     YLRSDPQRLLATILVGDRLVSTAIPMYATFLTLNKFGAERTFFDDTIAVVVGLLTFVL
                     LVSVDVIPKTLAAKFSVPVTLNMAYPVYGVQVCLKPILAIMVPFIHKLTGGKGLTLPL
                     VTEEELKIMLDEGGKAGAIEVEKVKMIKNVFQLKDITAEDAMTPRLYVFSLDGNLRLK
                     EAQELLYNSKYSRIPVYDGTLDNITGILYKTRALTELAKGQSDAKLKDIAYPPIFVPA
                     GKTADDLMKQFQQEKRHMAVVVNEFGGVMGIVTLEDLLEEVVGEIMDETDITEELIKR
                     LGKNQILVHGRTEVRKVNDFLKVDLGDEAVTIGGLIQQELGRIPKVGEELRIGNCRIV
                     IHEAEPRSIRSVQIFREEKHPVPVETPNLNLVS"
     gene            complement(1297..3309)
                     /gene="ligA"
                     /locus_tag="VLD60_08470"
     CDS             complement(1297..3309)
                     /gene="ligA"
                     /locus_tag="VLD60_08470"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSB45031.1"
                     /translation="MATTDVQARLTTLRDEIRRHDYLYYVKARPEISDAAYDRLFREL
                     TELERAHPELVTIDSPTQRVGAPPLDELKKVRHEQPMLSLDSLLDQADVLAFDQRMKR
                     ELDAPSVVYTAEPKFDGLSVELLYEQGLFVRGATRGDGTVGEDVTVNLRTIRSLPLQL
                     LPQSGYPAHVVVRGEVYMRLDDFQVLNRRITERGEEAFANPRNAASGSLRQLDSAITA
                     ERPLVVTCYEVMVHSGPALTSHWDELDALSRWGFPVPLHRRRCASIEDVIAYHHETES
                     IRDSLTYEIDGVVVKVDRRDWQEQLGFKSRSPRWAIAYKFTPRKEITVVQDIAVSVGR
                     TGTLTPIALLTPVEVGGVTISRATLHNADEVARKDIRIGDTVKVERAGDVIPAIVERV
                     PVPGERRREPFRMPDTCPVCQSTVAREGAYYYCTGKSVCMAQIKGAIEHFASKGALNI
                     EGLGKKTVAQLVDAGLVRSLADLYRLTKSQLLALEGFADRSASLLLESIERSKTVSLD
                     RFLMGLGIRQVGQHVAKVLARKFGSLSGIMEADHERFQQVKEIGPEIASSLVSYFSEA
                     HNRQVIQHLIECGFQIATSTVVPSADAVLAGKSFVFTGGLAHLTREQAKQLVEDRGGQ
                     VSSSVSKRTSYVVAGADPGSKLDQANKLGVTVLTEQEFLELVEGRL"
     gene            3565..4221
                     /locus_tag="VLD60_08475"
     CDS             3565..4221
                     /locus_tag="VLD60_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSB45032.1"
                     /translation="MKEVSQVRKSILKPAARVLTVICGGLVLSGCVLTEKYEAEKARS
                     LNFQRLLAQEEKRTGELDNEVKRSKRELAEYEAKTRELTAQLQAVREQMARVQEEADA
                     MKEAALLERKAHVDMKRSAPASAKSSPKESFPKESVPSINELLGSTPGSQGDAPEAQA
                     PTEELGESKAGPNIHVVKPGETLYRISRRYGVKVDTLRKWNKLPDDIIEVGQKLIVSQ
                     "
     gene            4238..5734
                     /gene="trpE"
                     /locus_tag="VLD60_08480"
     CDS             4238..5734
                     /gene="trpE"
                     /locus_tag="VLD60_08480"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HSB45033.1"
                     /translation="MPTPAYSLSLDQFRHYAREGNLIPIYREILADYDTPVSAFAKID
                     HGTTAYLLESIEGGEKWARYSFLGSGSSVVIYEDRGDLIYLRGKRKKRIPSRGNPLER
                     LRELMGDYRPVTVPNLPRFVGGAVGYLSYDIVRTFEELPGRRKDHLNLPDFAFLLTDT
                     LLIFDNVAQKIKVVANAHVTSGRDRDIKKAYRAATERIERMIARLKRPLRHARPHRRR
                     KPVAFTSNMSKADFEKMVVRTKEYIRAGDVVQAVLSQRWETNIQAPPLQLYRALRVVN
                     PSPYMYYLRVAGVELVGSSPETLVRCEDGVISVRPIAGTRRRGLSPEEDQQLERRLLA
                     DEKERAEHVMLVDLGRNDVGRVARQGSVTVDSLMHVERYSHVMHIVSNVTGRLDPSKT
                     SYDVLRACFPAGTVSGAPKIRAMEIIDELEPTRRGPYAGAVGYFSFSGNMDMCINIRT
                     VVIKKHQAFVQAGAGIVADSNPEYEYEETCNKARAMMKAIELAEQGLE"
BASE COUNT         1349 a         1677 c         1705 g         1205 t
ORIGIN      
        1 tattgcgatc ttattgggcc ttattgttct ttctgccgtg atttcgacgg cggagatcgg
       61 cttcttcgcc gtcaatgaga ccaagctgcg tgctcttgcc cagaacaaca acaatcgggc
      121 caagatggcc ctctatcttc ggagcgaccc gcaaaggctg ctggctacta tcctcgtcgg
      181 agaccgtctg gtgagcacgg cgattccgat gtatgccacg ttcctgacat tgaataaatt
      241 cggggctgaa cgaacatttt tcgacgatac cattgcggta gtcgtgggac tgctcacttt
      301 cgtcctgctt gtatcggtcg acgtcattcc caagaccctg gcagcaaagt tttccgtccc
      361 cgtcaccttg aatatggcct atccggtcta tggggtacag gtctgtctca agccgatcct
      421 ggctatcatg gtcccgttca tccataagct cacgggcggg aagggattga cccttccgct
      481 cgtaacggaa gaagaactca agatcatgct ggacgaggga gggaaagccg gggcgatcga
      541 ggtcgagaag gtcaagatga tcaagaacgt cttccagctg aaagatatta cggccgaaga
      601 cgcgatgacc ccgcgcttgt acgtcttttc gctggacggg aatcttcgtt taaaagaagc
      661 gcaagagttg ctctataact ccaagtactc tcgaatcccg gtttacgacg gcacgctcga
      721 taacatcacg ggcattctct ataagaccag ggccttgacg gagctggcga aaggtcagtc
      781 tgatgccaag ctgaaagaca ttgcctatcc gccgatattt gttccggccg gcaagaccgc
      841 ggacgatctc atgaaacagt ttcagcagga gaaacggcac atggccgtcg tcgtcaacga
      901 attcggcggc gtgatgggga tcgtcacgct cgaagacctg ctcgaagagg tcgtcgggga
      961 gatcatggac gaaacggata tcacggaaga attgatcaag cgcttgggga agaaccaaat
     1021 tctcgttcac ggccggacgg aagtcaggaa ggtcaacgat ttcttgaagg tcgatctcgg
     1081 cgacgaagcc gtgaccatcg gcgggctgat tcagcaggaa ctcggcagga tccccaaagt
     1141 cggagaagaa ctacgcatcg gaaactgccg tatcgtcatt catgaagccg agccgcgttc
     1201 gatccgcagc gtgcagatat tccgggaaga aaagcacccg gttcccgttg aaacgcccaa
     1261 tctcaacctc gtgagctagc gcgagcgcgc acagcttcag agacgtcctt ccaccagttc
     1321 gagaaattcc tgttcggtca ggaccgtcac ccccagctta ttggcctggt caagcttcga
     1381 ccccgggtct gcccctgcaa caacgtacga cgtccgtttg ctgacactgg aagagacctg
     1441 gccgcctcga tcctcgacta attgcttggc ctgttctcta gtcaagtgtg ccagccctcc
     1501 cgtgaagacg aagctcttgc ctgccaacac tgcatcggcg gaagggacga cggtgctcgt
     1561 ggcaatctga aagccgcact cgatcaggtg ctgaatcacc tgtcgattgt gcgcttcgga
     1621 aaaatatgat acgagactgg aggcaatctc aggccctatt tctttcactt gttgaaaccg
     1681 ttcgtgatcg gcttccatga tcccggatag cgagccgaat ttccgggcca ggacctttgc
     1741 gacatgttgc ccgacctgcc tgatccccag ccccatcagg aaacggtcga gtgaaacggt
     1801 tttgctccgc tcgatcgatt ccagcaagag actcgccgat cgatcggcga atccctccaa
     1861 cgccaatagc tgggatttcg tcagcctgta gagatcggcc aatgatcgga ccagtcctgc
     1921 gtcgaccaat tgggccacgg tcttcttgcc cagcccttca atgttgagcg cgccctttga
     1981 tgcgaagtgt tcgatggcac ctttgatctg cgccatgcac accgatttcc cggtgcaata
     2041 gtagtaggcg ccctcgcgcg cgaccgttga ctggcagaca gggcaggtat ccggcatgcg
     2101 aaacggctcg cgacgccgtt cgccgggaac aggaacccgt tcgacaatcg ccggtatgac
     2161 gtcgccggcc cgctcgacct tgaccgtatc gccgatgcgg atatctttcc gcgcgacctc
     2221 atcggcattg tgaagcgtcg cgcggctgat ggtgacaccg cccacctcaa ccggggtgag
     2281 gagcgcgata ggcgtcaagg taccggttcg cccgactgac acggcgatgt cctgcacgac
     2341 cgtgatctct ttccgcggcg tgaatttata cgcgatggcc caacgggggc ttctcgattt
     2401 gaatccgagc tgttcctgcc agtcccgccg gtctaccttg acgaccacac cgtcgatctc
     2461 atacgtgagg ctgtcgcgaa tcgattccgt ttcatggtgg taggcgatga cgtcttcgat
     2521 cgaggcgcag cgccggcgat ggagtggaac cggaaatccc catctggata aggcatcgag
     2581 ctcatcccag tgagacgtca acgcggggcc cgagtggacc atcacttcat agcaggtgac
     2641 gacaagcgga cgctctgccg tgatcgcaga gtctaactgc cgcagcgatc cggaggcggc
     2701 gttccgggga ttggcaaagg cttcctcccc ccgttcggta atccgcctgt tcaaaacctg
     2761 aaaatcgtcc aatctcatgt acacctcacc ccgcacgacc acgtgcgcgg ggtatcctga
     2821 ctgcgggagc aactgaagag gtaacgaacg aatcgttctc agattgaccg tgacgtcttc
     2881 tcccactgta ccgtcgcctc gcgtcgctcc ccgtacgaac agcccctgtt catagagtaa
     2941 ctcgacggag agaccgtcga atttcggctc ggccgtgtac acgacggaag gggcatcgag
     3001 ctcccgcttc atgcgttgat cgaaggccag aacatcggcc tggtcgagga gcgaatcgag
     3061 gctcaacatg ggctgttcat gccggacctt cttcagctcg tcgaggggag gggcccccac
     3121 tcgttgagtc ggggaatcga tcgtcaccaa ctccggatgg gcccgctcga gctcggtgag
     3181 ctcccggaac aggcggtcgt aggcagcatc ggaaatttcc ggacgcgcct tcacgtaata
     3241 gaggtaatcg tggcgacgga tctcatcccg cagggtagtc agtcgagcct gaacatcagt
     3301 ggtagccatc gaggtggttg aacaggagat atgggggtag aaaccgtgac tctatcggga
     3361 tcggtcgatg ctcgaagcga ccagacggta acatagcaag agggaaccgg tcaaactatg
     3421 gagccgcccc aatcgctttg actttcgcag cgttagccac tattctatcc aatcctgttg
     3481 gtcggatttt gaagagacag gaaccagaaa gcaattcccc gcgtcagaaa cgtgaaagcc
     3541 tcaattgcga tggaggaggt gtacatgaag gaggtgtctc aagtgagaaa gtcgatactg
     3601 aaacctgctg cccgagtcct caccgtgatt tgcggaggcc tcgtcctcag cggctgcgtt
     3661 ctcactgaaa aatacgaggc ggaaaaggcc cgcagtctga actttcaacg cctgctggct
     3721 caagaagaaa aacggaccgg ggaattggac aatgaagtga agcggagcaa acgggaattg
     3781 gcggaatacg aagcaaaaac ccgcgaattg accgcccagc tgcaggccgt ccgtgaacaa
     3841 atggcccgtg tgcaggagga ggccgatgcc atgaaagaag cggctctcct tgaacgcaaa
     3901 gcccatgtgg atatgaagcg ctcggcaccg gcttcagcaa aatcctcccc gaaggaatcg
     3961 tttccgaagg aatccgttcc gagcatcaat gaactgctgg gaagtacgcc cggttcgcag
     4021 ggagatgcgc cggaagctca ggctccaacg gaagagctcg gagagagcaa agccggtccc
     4081 aacattcacg tcgtcaagcc cggagaaacc ctctatcgca tcagccggcg ttacggggtc
     4141 aaagtcgaca ccctgcgaaa atggaacaag ttaccggacg atatcattga agtcgggcag
     4201 aagttaatcg tcagccagta gcgagaggac cattgttgtg ccgacgccag cgtactcact
     4261 cagtcttgat cagttccgcc attacgcccg cgagggaaat ctgattccga tctaccggga
     4321 gattctggcg gattacgata cccccgtgtc ggcgtttgcc aaaatcgacc atggaaccac
     4381 cgcctacctc ttggaaagta tcgagggagg cgagaagtgg gcgcgctatt cctttctggg
     4441 aagcggttct tccgtcgtca tctatgaaga tcgcggggat ctgatctatc tgcggggcaa
     4501 gcggaagaaa cgcatcccca gccggggcaa tcctctcgaa cgcctgcgcg aactgatggg
     4561 cgactaccgt ccggtgacgg ttcccaatct gccgcggttt gtcggaggcg ccgtcggcta
     4621 tttgagctat gacatcgtca ggacgtttga ggaattgccc ggccgcagaa aagatcacct
     4681 taatctccca gacttcgcgt tcctgctgac cgatacgctc ctcatcttcg ataacgtggc
     4741 gcaaaaaatc aaagtcgtcg ccaatgcgca cgtaacgtcc ggacgcgatc gggacatcaa
     4801 gaaagcctat cgggctgcaa cggagcggat cgagcggatg atcgcgcgat tgaaacgtcc
     4861 gctccggcac gcgcgccccc atcgccggcg taagccggtc gcctttacct cgaacatgag
     4921 caaggcggat ttcgaaaaga tggtggtgcg gaccaaagaa tatattcggg ccggagacgt
     4981 cgtgcaggcc gtgttgtccc agcgttggga aacgaatatc caggcgccgc ccctgcagtt
     5041 ataccgggcc ttgcgcgtcg tgaatccgtc gccctacatg tactacttga gggtggcagg
     5101 cgtggagctg gtcggctcgt cccctgaaac gctcgtgcgt tgcgaagacg gagtcatctc
     5161 cgtcaggccg atcgccggta cgcgccgtcg cggactgtcg ccggaagaag atcagcaatt
     5221 ggaacggcgc ctcctggcag acgaaaaaga gcgggccgag catgtcatgc tggtcgattt
     5281 gggtcgcaac gacgtcggac gtgtcgcacg ccaaggctct gtcacggtcg attcgttgat
     5341 gcatgtcgag cgctattcgc acgtcatgca catcgtgtcg aatgtgaccg gccggctcga
     5401 tccgtccaaa acctcctatg acgtgttgcg ggcctgtttc ccggcaggaa cggtttccgg
     5461 agcgccgaaa atccgcgcca tggaaattat tgatgaattg gagccgacca ggcgaggtcc
     5521 ctatgcggga gccgtcggct atttcagctt ttccggcaac atggacatgt gcatcaacat
     5581 ccgcacggtc gtcatcaaga aacatcaggc gtttgtccag gccggcgccg gcatcgtggc
     5641 ggattcgaat cccgagtatg aatacgaaga aacctgcaac aaagcccgtg cgatgatgaa
     5701 agccatcgaa ctggccgaac aggggctgga ataggaagag catgtggcga atggcatatg
     5761 gcacgaacaa gagtgaggcg cccgcgcgtg ctattagcca tcagccataa gctccgagca
     5821 gatcccgaat gatcacattt ctgtattgtg ctgagtccta agcgctgaca gctgatagca
     5881 ggatttaccg atgctgctca tgatcgacaa ctacgattcg tttacctaca atctcg
//
LOCUS       DATIFR010000139         1946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_475279, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000139 DATIFR010000000
VERSION     DATIFR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475279"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..881)
                     /locus_tag="VLD60_08485"
     CDS             complement(189..881)
                     /locus_tag="VLD60_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45034.1"
                     /translation="MKMTINPWGVGLMVAIAVYLMSLPSSEAQVRTFDAGVFKPLDRT
                     VTSTPAERTKVPAVNINPDQIRLSVQGFRRIEADFDVPEFNPRVAADPLQDNPNKYEL
                     GPDESAFVLSGWVFVNHDNYFGALPQFVAATTLFDDAPPECPVFNIPYPQAPKNAALA
                     NRRYKIYYVVRGTVSNPICASYFLNKATLEPGITAVRVSDGISVWADPTTYAATIIGR
                     SEKRIGAFYQKE"
     gene            complement(1092..1310)
                     /locus_tag="VLD60_08490"
     CDS             complement(1092..1310)
                     /locus_tag="VLD60_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HSB45035.1"
                     /translation="MRKDYDFSKSRKNPYAKGLKQQITIRLDPDSIRYFKDLAEETGI
                     SYQNLIDLYLKDCAREQRRLSIKWLKMG"
     gene            complement(1307..1603)
                     /locus_tag="VLD60_08495"
     CDS             complement(1307..1603)
                     /locus_tag="VLD60_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HSB45036.1"
                     /translation="MTDLVTFEWDEDKDRANVKKHGVSFEEASTVFWDENARIIDDPD
                     HSDEEERFILMGVSRNLNILVVCHCYRREGQVIRIISARRANKKELRSYKEHLI"
     gene            1683..>1946
                     /locus_tag="VLD60_08500"
     CDS             1683..>1946
                     /locus_tag="VLD60_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45037.1"
                     /translation="MIDLFLGAPSQKAAGMTLRYNEEGQAVFEMPYNPNYDHAMGAIH
                     GSVFGMMIDNAGWFTVAPHYENWIVTVEFSTRLHEPVSREDLWA"
BASE COUNT          439 a          546 c          525 g          436 t
ORIGIN      
        1 acctgtgggc cgtcgggcgg atcatcagga tgggcagccg gatggcctcg gcggagatgg
       61 aggtccggac ggcgggatac aagctcgccg ccacgggcgc ggggacgttc atcgtgactt
      121 cgacgcctta tcgggatggg acagccgacg atgtttaagg gccgactctt tccggatgga
      181 tcgacatcct attccttttg gtagaaggcc ccgatccttt tttcggatct gccgatgatc
      241 gtcgccgcat acgtcgttgg gtcagcccaa acgctgatgc cgtcgctgac acgcaccgcg
      301 gtgatgccgg gttccagcgt tgccttgtta aggaagtaac tcgcgcagat cgggttgctg
      361 actgtcccac gaacgacgta atagatcttg tagcggcgat tggccaacgc cgcgtttttc
      421 ggggcctggg gatacgggat attgaaaacc ggacattccg gcggtgcatc gtcaaagagc
      481 gtcgtggcgg cgacaaactg cgggagcgcc ccgaagtaat tgtcgtgatt cacgaagacc
      541 cacccgctca gcacaaaggc gctctcatcc ggtcccaact cgtatttgtt cggattgtct
      601 tgcaggggat ctgccgcaac gcggggatta aattcgggca catcgaaatc ggcctcgatc
      661 cgtcgaaatc cctggacgct caatcggatc tgatccgggt tgatgttgac cgccggtacc
      721 ttcgtccgct cagccggggt cgaggtcacc gtgcgatcga ggggcttaaa cacaccggca
      781 tcgaaggtcc ttacttgagc ctcgctcgac ggcagactca tgaggtagac cgcgatcgcc
      841 accatcaagc cgacgcccca aggattgatt gtcattttca tacgcccccc cgcgaggatg
      901 tgaagcaaat ttggtgccag gaattcaagg gcgagtgatg gagtaacgcg ttgaaattct
      961 gcgactaata ttcgccgacc ctccgaagag tattctcaaa ttgatagaat tctcagattg
     1021 ataacccgct gtggatccga accaagtaca caacaacacg cagggaaaac aggcgccagg
     1081 cacgcgtcga attaacccat cttcaaccac ttgatcgaca accgtctctg ttcgcgtgca
     1141 cagtccttca gataaagatc gataaggttt tgatacgaaa tgccggtctc ctcggccaga
     1201 tccttgaaat accggatgga gtccgggtcg agccgaatgg tgatctgctg tttcaggcct
     1261 ttggcgtaag gatttttccg tgatttgctg aagtcgtaat cttttctcat atcaaatgtt
     1321 ccttatagga gcgaagctcc ttcttattcg cccttctcgc cgaaatgatc cgaatgacct
     1381 gcccttccct ccgataacaa tggcaaacca ccagaatatt taggttccgg cttacgccca
     1441 tcagaatgaa gcgctcttcc tcatcggaat gatccggatc gtcaataatt ctggcgttct
     1501 catcccaaaa gacggtcgag gcctcctcga agctaacccc atgtttctta acattggccc
     1561 gatccttgtc ttcatcccac tcgaaggtga ctaagtcagt cataattata atataattat
     1621 actatctggc aatactttat aaggccgcca cgagacggcg cgaacatgaa gagcaatacg
     1681 gattgatcga cctcttcctg ggcgccccca gtcaaaaagc cgccggcatg accctccgct
     1741 acaatgagga aggccaggcc gtcttcgaga tgccgtacaa cccgaactat gaccatgcca
     1801 tgggtgcgat ccacggcagc gtcttcggga tgatgatcga caacgccggc tggttcaccg
     1861 tggcgcccca ctacgagaat tggatcgtta cggtggagtt cagcacgcgc ctgcacgagc
     1921 cggtgtcccg ggaggacctg tgggcc
//
LOCUS       DATIFR010000140        27086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1434024, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000140 DATIFR010000000
VERSION     DATIFR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27086
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434024"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VLD60_08505"
     CDS             <1..804
                     /locus_tag="VLD60_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45038.1"
                     /translation="ASMPKHVFQLPKDMYMVGYKSAVFTKAGTPLPRNYLHHILMLNN
                     DRPSVSCPGEPLFFSGAGLEMTEAKFPDGYGVKLGKGEHLMSVVAFYHKAPPTKDVVA
                     SFTMYMAPEGTPVKEMDVYQVGVNVVCYSKFKDRGPDQTDEGIEIKPGVQVHTAPLKF
                     SMDGCVKFAYPHGHDELLLISLENKTKNQTLLRTVPDVALDGTFLEFQPHQVYRDGHG
                     FPVTIADDYEMVMVHHHPLQKQELQHGMGNYLLYMTPGACPEKKTASAQ"
     gene            1138..5349
                     /locus_tag="VLD60_08510"
     CDS             1138..5349
                     /locus_tag="VLD60_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB45039.1"
                     /translation="MPPSEPVTILIVNDHADEIKLVTISLRGFFPDCRIDVAYSAVEA
                     ATMASATTAEWAVVLIDDGCLVDAPAAFVEDLKRRIPYAAILLQSGRTDSASALQALQ
                     VGADYFLSKHSPAFLTELLFCTKEALDTSELRRAAERTEIRHRQLVDSLGDIFYELDA
                     EGRFLSVSPNLVSLLGYRPEEVIGLSYLTVFSQSDQQIALFRFNERRSGARAISGFEL
                     TFLRKRPPGGAQILITAEVSARGLYSPFRRFLGTVGIIRDLSERKRQRTTIQELRQQL
                     ERAGEVRAIAQQIAALSKELQQPLSTLQNESRLLLETIREARVVDRLGSLSGHAAAAT
                     QLGKQLEHLVHETSRESAIDINRLLENVLMLSTEAGLEYEAAIVTDFAASLPAYKGDR
                     ERTIQLFQRLIDYARTYLEAVKRPRILIIRTSGVGPLPSVAGPTLFPLAPSRNLDIEI
                     LETGRHRPAGAPAPSLSEPIDLLDLYQRARELGASLDVSAPAAGPLRLAVRLPVEMQP
                     PLETAQPPAAAVSPSPLAAETPVSVQPPIIAPSPVEPVKPDRRNQPRISTTLPAHVTL
                     GSSAWEGTLNNLSLGGACLTLPSNFPSIALQEAYIVMRTAVGILELTGLVYERADSIA
                     SQATASIRPHIVIVFHPPRHTEAAVLASLIDAARERSLGFTLEVFLAAGPPERKAAGP
                     KPVPDAVPDHDRREALRVSLALPLRLETVRHQEPASRLVAQMMNLSRDGACLLVKERP
                     EQIRGLVLLHFAPAFRTDQPGSHEPGAPETALPAQIVWSAPDPMAPSTLHPSGTSHAS
                     RVGVRFLQLTPHAEREIHRVIRQHLNDQRTADALSASASVVTVPRECRNARGQAIAIA
                     DDHLGQTVDADMPTVILAPGFGQTALDYAALSYYLAAHHLRLLRYDHTNHVGTSEGEL
                     QHATMRSLQHDLLKVVEFVSHTWPKAPVLVVASDVAARAALKMAAQARPLDLLLLINP
                     SLDVGAMLMAMHGHDLIADYRFGLRRGIANLMGLNVDIDRFVGDLIAGHYSDLESTLD
                     DLRLIRSPLGIVTCPTPALTGLPPGDLSHAFVTALGAKTRLVNLPTSLTDHLLSAQEP
                     YPAAFARMLDLIVSVLPVRPKSPGPETTVYQQFSKERRVEQEYTCLRHEASHITREAL
                     CAAHLSQLPQFGNLHEYRKLLDDLYGLMSPLNPGAILVDAGIGQGDLTRAALVNHTYR
                     AGQTSWTGKPSPLMIGVARSAEMIGEARHAVQTLQRELSAGFIGRLAAMPPLNVGWIQ
                     ADWSISLPFKTGSLDRLVCNLSLPYVPSPLAALREWHRVLHPEGRLVLTTFHPHTDLS
                     MLYRRHLRQANQDEFNTQAQPLLHYFGRLREAICHRLLHTFDRPTLSSLLRQAGMTSF
                     RILPIFDGQALVAVVGKENSSSSIQ"
     gene            5454..6731
                     /locus_tag="VLD60_08515"
     CDS             5454..6731
                     /locus_tag="VLD60_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45040.1"
                     /translation="MAVQPTAIVIQPASSIDLLNLVTRFSKDMGAMTDVGALSTRIIE
                     ELCRAVSATRGALYLLDREHEWYRRAGTVGSAADSLFPATLPLTHPIPYHLSIEFRTV
                     SGITSSQDSLSDNQREDAQAAMAAMRVTRSLPHISRGRLIAFSLLDAGRSLEQDHASA
                     SPLLSALAQIATNALDTSMLLEDLHRSHLLMKRTDRLKSLETIAGGFAHEIRNPLTSI
                     KTFIQLAPERKDDPDFIREFSRIVLDDVCRIERLIEEILDYARYMEPKLTEEDVNDIV
                     SSCLYFIEVKADSRGIKIQKELASDLPRVMLDRQQIKQVFLNLFLNAVDAMSERGGTL
                     RVRTRKLLKPDGKQWVQIETEDTGHGIQAVNLEHIFDPFFTTKHESREHEGTGLGLTI
                     VHQIIQEHHGEILVRSTVGIGTTFLVNLPAIPL"
     gene            6758..8173
                     /locus_tag="VLD60_08520"
     CDS             6758..8173
                     /locus_tag="VLD60_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB45041.1"
                     /translation="MKKRVLLVDDEARVRASLKAVLEPTYEVFQAGDAQEALDLFHKE
                     APHLILLDVILPGTDGLSVLQSLRADQNPTPVIMLTGTKSVKTAVDAMKLGAADYLSK
                     PFDVEELRIVVDRALNSQELEREVKQLRAQVTQRYAFHNLIGKSRPMQEIYGKIEQVA
                     DSRTTVLITGESGTGKELVAKALHYNSGRRDRPFIALNCAALPETLIESELFGHEKGS
                     FTDATARRVGQFELANSGTLFLDEIGDLSPMTQAKLLRVLQEREFTRIGGVQPIKVDV
                     RIVAATNKNLEELVRKGQFREDLYYRINVISLFLPPLRERGEDIPLLAKHFLSKRIEE
                     ESRPSQEFSKDAVDLLSRYPWPGNVREMENIIEQAFIWSKGSDTVTPDHLPTILRNDT
                     RSSSLRDDTLAGRLSLEKAVMEFEREIILDALKKNNYVQTHAANQLGISRRMLKYRMD
                     TLGIGRPDSETVPEPEPLVQE"
     gene            8378..9532
                     /locus_tag="VLD60_08525"
     CDS             8378..9532
                     /locus_tag="VLD60_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSB45042.1"
                     /translation="MMTHSLTPETKREKPTVLVIDDEAGPRDALKVILRPFFNIQSAE
                     SARAALDVLNKQQIDLITLDQKLPDRQGLDLLQEIKHDHADIEVIIITGYGSLKSAME
                     GIRHGAAGYLLKPFNVTELITLVNQTLEKKQRLDYLRNFLASSTDLWGTEAECAKAWK
                     EMRAGYDAIGKPGGDLASSTGEPTDLLPMLSDLLEAKDRQLLNHCSRVSFYATLLANR
                     TTLTINEQKSLALGAFLHDIGKIGKVPYRFADDEIVATTGVSELRQHPELGARLVGPL
                     GLPAEIGQIIAYHHERWDGSGYPHGLKGEGIPVLARIVCLAQTFDHLTAELPERTALS
                     IDETSRFMAAHAGTYFDPALTEVFTQVVQECKASLPAMAIATNTSSRTDG"
     gene            complement(9541..11226)
                     /gene="recN"
                     /locus_tag="VLD60_08530"
     CDS             complement(9541..11226)
                     /gene="recN"
                     /locus_tag="VLD60_08530"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HSB45043.1"
                     /translation="MLAELRIVNFALIEQLHIRFQPGFTVLTGETGAGKSLLIDAIGL
                     LVGGRASTEQIRSGEDEAQLEARFHLPATHSLLQHLRSQDIIGPKDNELILRRILSRS
                     GRHRVYINGSLCPLRVLEDLGGTLVDIHGQHEQQSLLAGANQLEAVDGFGTILPLRAR
                     YEDAYREWNDLSGQIEAMHQAATGRDRLEEMLRFQVQEIDQAGLQDGEEAALHSERQR
                     LVHAHRLRELAEEAYAQMQADEEGVLVRLGRVSRHLGELKQTDPAMQECEQQAREALI
                     QLKDLSIRLREYADQLEANPDRQEQLEDRLDLIQRLKKKYGGSIDAVLATAAQARAEL
                     EGMETHESRVSELTSKRDAAFQLLSDLAKQLTRKRREAAKRLRGLVAAELEALKMEQS
                     ALEIEVLSDDVPDAFGPTGRDRVEFLLSGNIGEPPKPLSRIASGGELSRIMLALKTVL
                     AETDHVPVLVFDEIDTGVGGAVAAAMGTRLRKLGGFHQVFCITHLPQVASQAQHHLLV
                     EKSQDGKRTMTSVRSLSGIGREEEIARMLGGTTITKKVRETAAELIAGAKEKL"
     gene            complement(11303..11650)
                     /gene="trxA"
                     /locus_tag="VLD60_08535"
     CDS             complement(11303..11650)
                     /gene="trxA"
                     /locus_tag="VLD60_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSB45044.1"
                     /translation="MAGNVLKVEDGTWDAEVIKAPELVMVDFWAVWCGPCQMVAPIID
                     ELANEYAGKVKVRKLNTDENPEVAGRYQVMSIPTILFFKNGQVVEKLVGARPKRQFKE
                     MIDQLLAQHAGSA"
     gene            complement(11753..12298)
                     /locus_tag="VLD60_08540"
     CDS             complement(11753..12298)
                     /locus_tag="VLD60_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45045.1"
                     /translation="MMIHVSKVPVVRAMCCSLMLTCLWFGCSSLPPSASQAHPTNLQR
                     ARIFLAAGDYRRAVEACRQEVQEHPSAESYLYLTYVYQALDAYVESLAKMDQWVTMEH
                     LYLNLTSGSPEDLVDPPNVLARIAKEMIQSAAQQQADVTAELATRLDAAIVARVWPQQ
                     TAWRKINPNGWWFAPPPEWAW"
     gene            complement(12295..14784)
                     /gene="lptD"
                     /locus_tag="VLD60_08545"
     CDS             complement(12295..14784)
                     /gene="lptD"
                     /locus_tag="VLD60_08545"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HSB45046.1"
                     /translation="MAVSCRQFGADMAGVLTRILGCLTCVVVIGLMIPPWGTAAGSAG
                     PAPVPSSGAVPLNIKADRLDYLQDQEIYEADGSVVVDQGALHLTADHVTVQALPGVMI
                     ATGHVRLTDPKADLAAERLELNVNTEAGVITHGHVYIKASNTTVEGRLIQRFSEDHYR
                     VKEGRFTNCDAVEGEIPAWRFQFKDLDLNAGDSVAFTGGWLCVADAPVIPIPTMTYPL
                     SSRRSGLLIPTVGYDNRFGTHLQQGFYWAFSPSQDLTIAPSYYAQLGYGSDFEYRYVL
                     DKKSRGQWFVSYLQQTELPDVSGVSPTGQSAKQARAMITGTHTQQFTPDLLLRAQANF
                     VTDPNYLQQLSNSGAQRALPSNESNLLATQRLAYGNTYLLSQYLQPLQSGGVDTFQRL
                     PELGYSLPNLSLFNSSLLLGGDASAVNFYREQGFQQNRVDVVPGLSTDVIDFGHIVGI
                     TPQAKFREVYYTRGVQDTSSQHRETFWAGIDATSKLSRRFWSGDGNALLHTIEPSVMY
                     EYVPPSAQEQITQIDQVDDLPKKNLMTYMLRSRLLEQDGNRSFNWLDLTMAQSYHVGA
                     VQNQARNFAPGVTPPFGTVTQPLQPATVAVDGKKFSDLWLRAVIGNNSPQITQSQLAG
                     QTFGRGAGATPAIKPVINQYLTIDATYDPYRSTMSQFNTDFRVQESNNWYLDVGQRYS
                     ISGNRLRRGDIWNPISFNEVYAPTEELLFTTVGGAFRTPWGWTVGAKGYYDVKHGRSP
                     EYDIVGLYQNPCKCWSLGLYYLQFPDRAQYNFMISLTGIGWTENYGTVVLKEILSPLL
                     WGERGLPWAAPGGPYGRPQSVQPQPGPGGPP"
     gene            complement(14753..16048)
                     /locus_tag="VLD60_08550"
     CDS             complement(14753..16048)
                     /locus_tag="VLD60_08550"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSB45047.1"
                     /translation="MTYAESVAYLYSLQKHGIKLGLETMTALVSRLDMPQIRYPTLHI
                     AGTNGKGSTAAMTAAILQAAGYRVGLYTSPHLIEFRERIRVNGEMIAEAAVARLTEQL
                     RALCQPDLSPTFFECTTAMAFQHFADAKVDIAVMEVGLGGRFDATNMVIPLACTITTI
                     ALDHQEYLGTTLSSVAFEKAGIIKPQVPVIVGRVPGEAFHTIERVALEGGASLRRLGR
                     EFRVRGWTPECFSFTGSTLQVDDLHCTLQGLHQIDNAACALALLESAASGGVAITEKA
                     VREGLSNVRWEGRIEIVDREPMVVLDGAHNPAAAAALAEYLCASRLSRPGRRIILVLG
                     MMRDKDHRGFVEPLNDLVDEVVLTQADLPRSATAQELRAKIGDCWPHAFVKEVLSDAL
                     AQARGLAGADDLICITGSLMLIGDVKALFRGCELSPIRG"
     gene            complement(16071..16907)
                     /gene="accD"
                     /locus_tag="VLD60_08555"
     CDS             complement(16071..16907)
                     /gene="accD"
                     /locus_tag="VLD60_08555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSB45048.1"
                     /translation="MAWFKKQKGSEPEAPKRSKVAEGMWLKCNHCREIVYRKEVERNN
                     KVCPKCDYHFPISVMERITLLVDFGTFKEWDADLESQDPLAFHDTKSYKDRVKAQQEK
                     TGRKDAIMIGEGQMNGRRVALCVFDFGFMGGSMGSVVGEKICRAIDRALEARLPVVLV
                     TASGGARMQEGILSLMQMAKTSAAVAKLGEAKLPFICLLADPTFGGVTASVAMLGDVI
                     IAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLEHGMIDMIVERKQLKETLSTLVA
                     HF"
     gene            complement(17054..17761)
                     /locus_tag="VLD60_08560"
     CDS             complement(17054..17761)
                     /locus_tag="VLD60_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45049.1"
                     /translation="MIEPSFLTLLSLGFMLGLRHALDSDHVAAVSTVLTQRPSLKASG
                     LIGFSWGIGHTLILLLVGLIVLWFRVPVPELLAQVAEGAVGVMLVVLGGLLGVKLFKE
                     RWHVHQHDHDGSRHVHLHSHAVVVDHGHPHWWRESVRPLCIGMAHGLAGSAALLLLVV
                     SSARSVMEGLVYIMVFGCGSILGMMLIGLALSLPVVWSLQLGRPVFLAIQGLASLGSV
                     GLGLSIVYRILMGEQLS"
     gene            complement(17957..19021)
                     /gene="moaA"
                     /locus_tag="VLD60_08565"
     CDS             complement(17957..19021)
                     /gene="moaA"
                     /locus_tag="VLD60_08565"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSB45050.1"
                     /translation="MNEAIDSHSLPTVVDTYGRPLRSLRLSVTDRCNLRCAYCMPEED
                     YIWLPREDVLTFEEMAELTGYFTDLGVDKVRLTGGEPLLRRDVPRLIRLLLQNRRITE
                     IALTTNGTLLSDQIEALYEAGLHRVTVSLDTLRPERFRQLTRRDEYARVLEGIESVGR
                     AGFTGLKLDTVAIRGFNDDELVALIEFGKHYQAEVRFIEYMDVGGANEWAPEKVLSRE
                     EMLTTLGKYYGKIEPIPERGSAPAQRFLLSDGTTFGIIPSTTTPFCSTCDRSRVTADG
                     MWYRCLYATTGTDLRKPLRNGMSADDMRAFIRAGWEARRDRGAEERKALERVGLREGG
                     LIGIERLREDPHLEMHARGG"
     gene            complement(19043..19741)
                     /locus_tag="VLD60_08570"
     CDS             complement(19043..19741)
                     /locus_tag="VLD60_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSB45051.1"
                     /translation="MITVRLFGMTKSLAGNQVSLSLALANGQRVKDLVGLVNEGYPMI
                     GELIHKKKILVSVNQEIAHEDTEVREGDEIALLPPFAGGSLMEQTLDESQLVRVQRED
                     FSVDAEINRVRSRSKRIGGISIFLGTARDRSKGRDVDSITFEHYEGMAQKKLCEIRER
                     ALKDFDILELLIVHRYGEIAIGENIVLIIAGAEHRAEAFRACKWAIDELKQITPIWKL
                     EHTPSGEVWVEEHP"
     gene            complement(19755..20249)
                     /gene="moaC"
                     /locus_tag="VLD60_08575"
     CDS             complement(19755..20249)
                     /gene="moaC"
                     /locus_tag="VLD60_08575"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSB45052.1"
                     /translation="MAEFTHFNESGRARMVDVSAKARSERVATAQAKVYLQPETLEKI
                     QRGKIAKGDVLAVAQVAGVMGAKKTPDLIPMCHPLFLSSVDISFTEEPLPDRNGRCAI
                     IVTATAKTTGQTGVEMEAMTAASVAALTIYDMCKAIDRGMSFSDICLLSKSGGKSGTY
                     TREG"
     gene            20534..21484
                     /gene="folB"
                     /locus_tag="VLD60_08580"
     CDS             20534..21484
                     /gene="folB"
                     /locus_tag="VLD60_08580"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSB45053.1"
                     /translation="MTGKIVIERLEFQGRCGITPEERLRPQPVAVDLELDCDTALAGA
                     SDRISDTVDYAQVAERIVELGATQDCALLEALAENLLTMLFTDFPVERVKLWLRKLAP
                     PLTQMTGSVGIKVERARMAHHAQQTDPSPSRFLTQQLHRLPKGRVLDVACGSGRHTLY
                     LASHGFDVEAIDRDPDNLTKLASAAKQRHLSNVTVRQVDLERATDERPEFPANQYDAI
                     VVCFYLHRPLFPWLIEALKPHGVLLYETFTIDNYFRHRHPRRWEFCLAHNELLRLTSS
                     LRVLSYDEGEHEGGPGMGSVFTAQLIAQKPGPAGSLHEPH"
     gene            21471..22319
                     /locus_tag="VLD60_08585"
     CDS             21471..22319
                     /locus_tag="VLD60_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSB45054.1"
                     /translation="MSRIDLHLHTTHSDGSLRPSDVLALARQANVSALAITDHDITTG
                     IPEAMATGETLGIEVIPGVEISSFDGKSELHILGYCVRWQDAAFNQRLATLRESRHRR
                     NPLIIERLREAGLDVNYEEVRALAGTESVGRPHIAQLLMQKKYVSSAKEAFDRYLAEG
                     RPAYVARELPTPAEAISWIREAKGVAVLAHPTWVKESGEGLRTCVTTLKEAGLGGIEV
                     HYSTHSKSQTAGYLDLAHRLDLLITGGSDFHGLTKPDIEVGSGRGDLQVNPRLLAPLK
                     EAAARI"
     gene            22415..23815
                     /locus_tag="VLD60_08590"
     CDS             22415..23815
                     /locus_tag="VLD60_08590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSB45055.1"
                     /translation="MSKHLPWVLPYGAGILAALLVSPLLSSMAWVEFFSIPLTPVTGP
                     DALRLTADAIVLGLVLSLAVTAQQRLSDNGRGSGFLREILFPLATLLVVIFGGKALRT
                     AGFPLVQQIGPSPFSWGYALALLGSGLWTTVAWLRHQDMLRNAFTPPPAARRLTKHTE
                     QVETHEPSDSSNELATQPKEKAPAVTANGTPPATLGRYKILKELGRGAMGLVYLGKDP
                     TIQRFVAIKTMRLDQIDEEKKLQENKSRFFREAESAGRLSHPNIVTIYDAGEQDELGY
                     IAMELVEGKSLKEWSRKPNLMPVAEVVQNLSTVADALDYAHQQGVVHRDVKPANIMIT
                     KDRLLKVMDFGIAKVVSSSKTQTDVVLGTPTYMSPEQIAGKKVDGRSDIFSLGIVLFE
                     LLTGQPPYTADNLSALLFAIAHQPHPQLHAVRQDLPPVLQEVLNRALQKELPQRYRRA
                     GEFAQDLRACLCSLAA"
     gene            23829..24653
                     /locus_tag="VLD60_08595"
     CDS             23829..24653
                     /locus_tag="VLD60_08595"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HSB45056.1"
                     /translation="MAIEALHSAKTDIGRKRPHNEDCFVGDQELGLYVVCDGMGGGNA
                     GEVASRMAIDTIMAHVKAAIEDPAVQLIGPVDPNLSPATNQLASAIRAANEAIHRASW
                     TNSKYAGMGTTVVAARLTEQHLSIAHVGDSRLYLIRDHILHALTTDHSWVAEQIAQGY
                     LTEEEAERSPRRNIVTRALGVEGSVEIDLTEIPILNGDMLLLCSDGLTRGVRSGDILQ
                     TLNIPTDLGAKADRLVSLANQAGGDDNITVLLVAIQRGATDRLWHRIRQRWFLKAS"
     gene            24674..25381
                     /locus_tag="VLD60_08600"
     CDS             24674..25381
                     /locus_tag="VLD60_08600"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HSB45057.1"
                     /translation="MAHTVTESATLLIKIKGKSSRTIDIAPETFTIGRKADNNLPIDD
                     HTVSGHHAKIVRVQSVYFLEDLKSTNGTFLNGKPIDRAQLHDADVITIGQHRIIFQDS
                     TITPAATSSQPVDLEQTMAISSKDSHAKPPTTTAKVLVTAGKTERLEYHLTKPANLIG
                     SQEGAAIRLTGWFAPKSAALVSNRGSIYAISPSQGMKKLQVNGENVSGQRQLNNGDII
                     EVAGVSMTFYLVPPKGK"
     gene            complement(25402..26025)
                     /locus_tag="VLD60_08605"
     CDS             complement(25402..26025)
                     /locus_tag="VLD60_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HSB45058.1"
                     /translation="MGQAHSSGRIVAGVVTVVGLGIVWLAILMSLPSQQELSAKEPKL
                     SQLPISASKAQLPSEPTLDLSIPGVPSVPPAPSEQNPAATVGASLSPLAQLNPREVQV
                     ARLRCEAEIEQLCPDSLEGPARRRCLEKRAQQLAGPCQQQLRERFVKWKEERTRLVTA
                     CQADVKRWCASMKPGDGQILQCLQDHAQEVSDRCYETLPKGTVYFKQ"
     gene            complement(26168..>27086)
                     /locus_tag="VLD60_08610"
     CDS             complement(26168..>27086)
                     /locus_tag="VLD60_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45059.1"
                     /translation="DTLQSFLEQGREHDLLTVLAALDEDMAEWLFDLVAEATCSILLD
                     GPVDEKLSYAVMAALELPLQANLTHPLKLKIDPVATADILHRHLRLSAGTVVRVEPRL
                     LTDATLEALTLQALNEHLGTVADSQRARSIAARLYPPVENTAFLFFELIGVPDSGLPD
                     SLEDPHRRALLEGLVEQCPELALAPDTLEAPVYAAYAMFDAQNAVRLHRLADETAAVW
                     RDLDPLVRARTIVHLHTQCGNGVYMPVWTDLFDPELPDEHGPVWTSPDVWVFTADLPI
                     EWPGEMLTNRLLAEGCTRFENHIVEAPEN"
BASE COUNT         6003 a         8347 c         7099 g         5637 t
ORIGIN      
        1 gcctccatgc cgaagcatgt cttccaattg cccaaagaca tgtatatggt cggatacaag
       61 tccgcagtct tcacgaaagc cggcacgccg cttcccagaa actatctcca tcacattttg
      121 atgttgaata acgacagacc cagcgtctcc tgcccaggcg agcccctgtt tttcagcgga
      181 gcgggacttg agatgactga agccaaattc ccagacggtt atggagtcaa gctcggcaaa
      241 ggggaacatt tgatgtctgt cgtggcgttt taccataagg cgcctccgac caaagacgtc
      301 gtggccagtt ttacgatgta catggcgccg gaaggcactc ccgtcaaaga aatggacgtc
      361 taccaggtcg gggtcaacgt cgtctgctac agcaagttca aagaccgcgg ccccgaccag
      421 acggacgaag ggatcgagat caaacccggt gtccaggtcc ataccgctcc cctgaaattt
      481 tcaatggacg gctgcgtgaa attcgcatac ccgcatgggc acgacgagct gttgttaatc
      541 tcactcgaaa acaaaaccaa gaatcagacc ttacttcgca cggttcccga tgtcgcgctg
      601 gacggcacct tcctcgaatt ccaaccccat caagtctacc gagacggcca tgggtttccg
      661 gtcacgatag cggacgacta tgagatggtc atggtccatc accaccccct tcagaaacag
      721 gaactacagc acggcatggg aaactatctg ctctacatga cgccgggggc ctgtccggaa
      781 aagaaaaccg ccagcgcaca gtaacgcatg tctccttgag tcggccgacg cgtgtacggc
      841 agactcggtc ttcctcactt cattcctcac cttcccgttt ccgcttgtcc atttttgttc
      901 gttcattcaa tttcgtgcac tcgcccccct accaaagcgg gagccatttg ctcgggatcg
      961 agactgtgct tctctctccc gcctgtgtta ttcacatagt tacaccgagc agcagatttc
     1021 ctccgccgcc ggaaaaaagt gggtcggtct gcctccggtc gcggcgcatg gagcatgggc
     1081 catccactcc ggaaccgcat ttgctccgat gcccgaaacg atgacggaga ccagtcgatg
     1141 ccgccaagcg agcccgtgac gatcctgatc gtcaacgatc atgccgacga gatcaaactc
     1201 gtcacgatca gcctgcgggg attttttcca gactgccgca tcgacgtcgc ctactccgct
     1261 gtcgaagcgg ccacgatggc ttcggccacg acagctgagt gggctgtcgt gttgatcgac
     1321 gacggatgcc tcgtcgacgc gcccgccgca ttcgtcgaag atctgaagcg tcgcattccc
     1381 tatgctgcaa tccttctcca aagcggacgc accgattccg cttccgccct ccaggcgctg
     1441 caagtcgggg cagactactt cctgtccaag cattcgccgg cctttctcac cgagctgctc
     1501 ttctgcacga aggaagcact cgatacgagt gagttgcgac gcgctgcgga acgaacggaa
     1561 atccggcacc gccaactggt cgactcgctc ggcgacatct tctacgaact cgatgcggag
     1621 gggcgtttcc tcagtgtcag ccccaacctg gtttcgctac ttggttaccg gccggaggaa
     1681 gtgatcggcc tctcctatct caccgtattt tctcaatcag accaacagat cgccctcttt
     1741 cgattcaatg aacgccgctc cggggcccgc gcaatctctg ggtttgaatt gacattccta
     1801 aggaaacgcc cgcctggtgg agcacaaatc ctcatcaccg cagaggtgag cgcaagaggg
     1861 ctctacagcc ccttccggcg attcctggga accgtcggaa tcatccgaga cctgtcggaa
     1921 cgcaaaaggc agcgcacgac gattcaagag cttcgtcagc agctggaacg tgccggagag
     1981 gttcgcgcaa tcgcccagca aatcgctgcg ctctcgaagg agttacagca acccctctcg
     2041 acgttacaga acgaatctcg actgctcctt gagaccattc gcgaggcacg cgttgtcgac
     2101 cgtttggggt cattgtccgg gcatgccgcc gccgccacac aacttgggaa acaactcgag
     2161 cacctcgtcc acgagacctc acgagagtcc gccatcgaca tcaaccggct cctcgaaaat
     2221 gtgctcatgt tgtcgaccga ggctgggctg gaatatgaag ccgccatcgt taccgacttc
     2281 gccgcgtccc ttccagccta caaaggtgat cgggagcgga ccattcagct ctttcaacgc
     2341 ctcattgact atgccaggac ctatctcgaa gccgtcaaac gaccccgaat tctcatcatt
     2401 cgcaccagcg gagtagggcc tcttccttcg gtcgctggtc cgacactatt cccccttgct
     2461 ccatcgcgca accttgatat tgaaatcctc gagaccggtc gccaccgacc agctggcgct
     2521 cctgctccct cgttgtcgga gccgatcgat cttctggatc tctatcagcg tgccagagag
     2581 cttggcgcca gcctggatgt ctcggcgcct gctgccggcc ctcttcgcct ggcggttcga
     2641 ctcccggtcg agatgcagcc ccccctcgaa acagctcaac caccggccgc agccgtcagt
     2701 ccctccccgc tggctgctga gacaccggta tccgtgcagc ctcccattat cgcaccgtcc
     2761 ccggtcgaac ccgtcaagcc tgatcgcagg aaccagcccc gcatatcgac cacgcttccg
     2821 gctcacgtca cgctcggctc ttccgcgtgg gagggaacgc tcaataatct cagcctgggc
     2881 ggtgcctgcc tcactctgcc cagcaatttc ccttcgatcg cacttcagga agcctacatc
     2941 gtcatgcgaa ctgccgtggg cattctcgaa ctcacgggac tcgtctacga acgagcggat
     3001 tcgatcgcct ctcaagcaac agcttcaata cgacctcata tcgtcatcgt attccatccg
     3061 cccaggcaca cagaagcggc ggttctcgct tctctcatcg acgcagcacg cgaacggtcg
     3121 ctgggcttta cccttgaagt gttcctggct gccggtccgc cagaacggaa agctgccggc
     3181 cccaagcctg ttcctgacgc tgtccccgat cacgatcgac gagaagcgct tcgcgtatcc
     3241 cttgcgcttc ctctccgtct ggagacggtg cgtcaccagg aaccggccag tcgcctcgtt
     3301 gcccagatga tgaacctcag tcgcgacggt gcatgcctgc tcgtgaaaga gcgcccggaa
     3361 cagatccgcg ggctcgtcct tcttcatttt gctcctgcat tccgaaccga tcagcccgga
     3421 tcccacgagc ctggcgcgcc cgagaccgcc ctccctgctc agattgtctg gtcggctcca
     3481 gatccgatgg cccccagcac gcttcatcca tccggcacgt ctcacgcgtc aagggtcggc
     3541 gttcgattcc tccagctcac tccgcatgct gaacgagaaa ttcatcgggt gatcaggcag
     3601 catctcaacg accaacggac ggcggatgcc ctctcagcat cggcatccgt cgtcacggtg
     3661 ccgcgagaat gccgaaacgc acgaggtcaa gcgatcgcga tcgcggacga ccatctcggc
     3721 caaacggtcg atgcggacat gcccaccgtc attctggccc ccggttttgg acaaactgcg
     3781 ctggactatg cggccttgtc ctattatctg gcagcgcatc acctacgcct cctccgctac
     3841 gaccacacca atcatgtcgg taccagtgag ggcgagttgc aacatgcgac gatgcggagc
     3901 ctgcaacatg atctcttgaa ggtcgtcgaa ttcgtcagcc atacctggcc gaaagccccg
     3961 gttctcgtcg tcgccagcga cgtcgccgcg cgggcggcgc tcaaaatggc cgcacaggct
     4021 cgtccccttg acctgctgct cttgatcaac ccatcgctcg atgtcggggc catgctgatg
     4081 gcaatgcatg ggcacgatct catcgcggat taccgcttcg gtttgcgccg tggcatcgca
     4141 aatctcatgg ggctgaacgt cgatatcgat cggttcgtcg gtgatctcat cgccggtcat
     4201 tatagcgatc tggaatcgac acttgacgat ctgcgtctca tccgatcccc gctcgggatt
     4261 gtcacctgtc cgactcccgc tttgaccgga ctcccgcctg gggatttgtc tcacgcattc
     4321 gtgacagctc tcggcgcgaa aacccgattg gtgaatctcc ccacctcgct gaccgatcac
     4381 ctcctctccg cccaggaacc ttatccggcc gcattcgcac ggatgctgga cctgatcgtc
     4441 tccgttctgc cggtccgacc caaaagcccc ggtcctgaga caacagtcta tcagcaattc
     4501 tccaaggagc gccgggtcga gcaggaatac acctgccttc ggcacgaagc ttcacacata
     4561 accagggaag ccctctgcgc agcccacctt tcgcaacttc cccaattcgg aaacctccat
     4621 gagtatcgca agttgctgga cgacctgtac ggtttgatga gcccactcaa tccaggtgcg
     4681 atacttgtcg atgccgggat tgggcagggc gacttaacgc gtgccgcttt agtcaaccac
     4741 acctatcgcg ccggacaaac cagttggacc gggaagccat cacctctcat gattggagtg
     4801 gcgcgatccg ccgagatgat cggtgaagct cgccatgccg tacagacctt gcaacgagaa
     4861 ttgtcggctg gatttatcgg ccgcttggca gcaatgccac cgctcaacgt cggctggatc
     4921 caagccgact ggagcatctc actccccttc aaaaccggct cgctcgatcg acttgtgtgc
     4981 aacctgtctt tgccatacgt gccttctcca ctcgccgcat tgcgggaatg gcatcgagtc
     5041 ctccaccccg aaggccgtct cgtcctgacg acgtttcatc cccataccga cctctcgatg
     5101 ctctatcgcc ggcacctccg tcaggcgaat caagatgagt tcaacacgca ggcccagccg
     5161 ctgctgcact actttggccg tctccgcgaa gccatctgcc atcggcttct ccatacgttc
     5221 gaccgaccta ccctttcctc actgctcaga caggccggca tgacctcatt ccggatcctt
     5281 cccatcttcg acggccaagc cctggtggcg gttgttggga aagagaattc ctctagctcc
     5341 attcagtaac ggggtgtata ctgcgtcgct gtacaacttt gaacagcggg atcactcgcg
     5401 catccagtta gcccagaatt ggttcagaaa tccgagtcgc ttcgcctact tctatggccg
     5461 ttcaaccaac cgcgatcgtc atacagcctg cgtcttcgat cgatctgctg aatctggtca
     5521 ctcgcttttc aaaagacatg ggggcgatga ccgacgtcgg agcattaagc actcgaatca
     5581 ttgaggaact ctgtcgtgcg gtctcagcaa cacggggcgc tctgtatcta ttggaccgcg
     5641 aacatgaatg gtatcgccgc gcaggaacgg tgggctctgc cgcggattcg ctctttcctg
     5701 ctaccctccc gttaacccat ccaatccctt atcatctgtc catagagttt cgaactgttt
     5761 ccggcattac gtcttcacaa gatagccttt cagataatca gcgagaagac gcgcaggccg
     5821 cgatggctgc aatgcgggtg acacgcagcc tgccgcacat cagcagaggc cggctgattg
     5881 cattttcgct gctcgatgcc gggcgatcgt tggaacagga ccatgcgtcc gcgtcgccac
     5941 tcttgagcgc tctggcacaa atagccacca acgcactgga taccagcatg ctgctcgaag
     6001 acttacatcg atcccacctg ctcatgaaac gcaccgaccg gctgaagtct ctcgaaacga
     6061 ttgccggcgg gttcgcacac gagatccgaa atccgctgac gtcgatcaaa acgtttattc
     6121 agctggcacc ggaacgaaag gatgaccctg atttcattcg cgagttcagc cgtatcgtct
     6181 tggacgacgt gtgccggatc gaacggttaa tcgaggaaat cctcgattac gcacgctaca
     6241 tggagcctaa gctgacggag gaagacgtca atgacatcgt gtcttcctgt ctgtatttca
     6301 tcgaagtaaa ggcagacagc cgcgggatca agattcaaaa agagttggcg tctgacctgc
     6361 ctcgtgtcat gctagatcgt caacagatca agcaggtctt tctgaacctc tttctcaacg
     6421 cggtggacgc gatgagcgaa cggggcggga cgttgcgtgt tcggaccagg aaactcctca
     6481 agccggacgg gaaacagtgg gttcaaatcg agacggaaga tacgggccac gggattcagg
     6541 ccgtcaacct cgaacacatc ttcgatccgt tcttcaccac caaacacgaa agccgagaac
     6601 atgaaggcac tgggcttggc ctaaccatcg tacaccaaat cattcaggag catcacggcg
     6661 agatcttggt cagaagcacg gtgggcatcg gcaccacatt tctggtcaac ctcccggcca
     6721 ttccattatg acattgaaaa acggtacgga caccaacatg aagaaacgcg tgctcttggt
     6781 cgacgacgaa gccagggtcc gagcctcgct gaaggcggta ctcgagccga cctatgaggt
     6841 tttccaggcg ggcgatgcgc aagaagcact cgacttgttc cacaaggaag ccccccacct
     6901 cattctgctt gatgtgattc ttcccgggac cgatgggctc tcggtcctgc aatcgttgcg
     6961 cgcagaccaa aatcccaccc cagttatcat gttgaccggc accaaatccg tcaaaacagc
     7021 tgtggatgcc atgaaactgg gcgcggcgga ttacctgtct aaacccttcg acgttgaaga
     7081 actccgtatc gtggtcgacc gtgcgctcaa ttcgcaggag ctcgaacgcg aagtgaaaca
     7141 gcttcgagcc caggtcaccc agcggtatgc cttccacaac ctcatcggaa agagccgccc
     7201 gatgcaggaa atctacggca agatcgaaca agtcgccgac agccggacga cggtcctcat
     7261 aaccggcgag agcggcaccg gcaaagaatt agtggccaag gccctccact acaacagtgg
     7321 gcggcgagac cgtcccttca tcgctctgaa ttgcgcggca cttcccgaaa ccttgatcga
     7381 aagcgaattg ttcgggcatg aaaagggctc cttcacggat gccacggccc gccgtgtcgg
     7441 ccaattcgag ctcgccaaca gcggcacgct cttccttgac gagatcggtg atttgagccc
     7501 catgactcaa gccaagctgc tacgggtctt acaagagcgg gagtttacgc gaatcggcgg
     7561 ggtccagccg atcaaagttg acgtgcgcat tgtggctgcc accaataaaa atctcgaaga
     7621 actcgttcga aaaggacaat ttcgagaaga cctgtattac cggatcaacg tcatctccct
     7681 cttccttccc ccgcttcgtg aacgcggaga ggatatcccg ctccttgcga agcatttcct
     7741 ctcgaaacgg atcgaagaag agagccggcc cagtcaggaa ttttccaaag atgccgtcga
     7801 tcttctctcg cgatatcctt ggcccggcaa cgtgcgggag atggagaaca ttatcgagca
     7861 ggccttcatt tggtcgaaag gctcagatac ggtcacgccc gatcatctgc cgacgattct
     7921 gcgcaatgac actcgatcgt cttcgctccg cgacgacacc ttggcaggac gcctctctct
     7981 ggagaaagcc gtgatggagt tcgagcggga aatcattctc gacgcgttaa aaaagaacaa
     8041 ctatgttcag acccacgctg ccaatcagct cgggattagt cgtcgtatgt tgaagtaccg
     8101 gatggatacc ttagggatcg gccgccctga cagcgaaaca gtccctgagc cagagcctct
     8161 agtgcaggaa tagcacacta cgtttcccct aactgagtga tttttgcgtc aataaatgtg
     8221 tatttctcct cacgaactac gtagactttc gttcatggaa actacatatg gggtttcctt
     8281 ccttgcccac cacatctgct tgagaaatcg cttataaata gctgaaacgg aacgagaatt
     8341 gcttgacaga acagatactt aggcgtacac gaacaggatg atgactcatt ctctgacacc
     8401 ggaaacgaag agagagaagc ctaccgtcct cgtcattgat gatgaggcag ggccccgcga
     8461 tgcccttaaa gtcattcttc gcccgttttt caatattcaa tctgctgagt ctgcgcgtgc
     8521 tgccctcgac gtgttgaaca agcaacagat cgatctaatc accctcgatc aaaaactccc
     8581 ggaccgacaa ggcctcgatc ttctccagga aatcaagcac gatcatgccg atatcgaagt
     8641 catcatcatc accggatacg gcagcttgaa atcggcgatg gaaggcatta ggcatggagc
     8701 ggccggctat ctcctcaaac cgtttaacgt gacggagctg atcaccctcg ttaatcagac
     8761 ccttgagaaa aaacagcggc tcgattatct gcggaatttt cttgcgtctt cgacggacct
     8821 ctggggcact gaagcagaat gcgccaaagc ctggaaagag atgcgcgctg ggtatgacgc
     8881 gatcggcaag cctggaggag acctcgcatc ctcgacagga gagccgacag acctgctacc
     8941 gatgctctca gacctcctcg aagctaagga tcgccaactg ttgaaccact gcagccgagt
     9001 gagcttctac gcaaccctgc tcgccaatcg aacgactctg accatcaatg agcagaaatc
     9061 cttggcgctt ggtgccttcc tccacgatat cgggaagatc gggaaagtgc cctaccggtt
     9121 cgctgacgat gagattgttg ccacgaccgg ggtgagcgaa ttgaggcagc accccgaatt
     9181 gggcgcgcga ttagtgggtc cgctcggatt gccggcagag atcgggcaga ttatcgccta
     9241 ccaccacgag cgctgggatg gctcaggcta tccgcatgga ttaaagggag aaggcattcc
     9301 cgttctggcg agaattgtct gcttagccca gacattcgac catcttactg ctgaactacc
     9361 cgaacgcacc gccctgtcta ttgacgaaac cagccggttc atggcggcgc acgccggcac
     9421 ctattttgac ccggctctca cggaagtctt tacgcaggtc gtccaggaat gcaaggcctc
     9481 gctgccagcc atggccatcg ccaccaatac ttccagccgc acagacggat aacccgccct
     9541 ctatagtttt tccttcgcac ctgcaatcag ttccgccgca gtttcccgaa ctttttttgt
     9601 aatagtcgtg ccgcccaaca tacgagctat ctcctcttcg cgaccgatcc cgctgaggga
     9661 tctgaccgag gtcatcgtcc gcttgccatc ttgactcttt tccacgagta gatgatgttg
     9721 agcctgtgat gcgacctgag gcagatgagt gatacaaaat acttgatgaa acccgccgag
     9781 ctttctcagt cgcgtaccca tcgccgcagc caccgctccc ccgacaccgg tgtcgatctc
     9841 gtcgaaaaca agcaccggca catgatccgt ctcggccaac acagttttca atgcgagcat
     9901 gatccgggaa agctccccgc ccgaagcgat tcggctcagc ggcttgggcg gttccccgat
     9961 gttgccggac agcaagaact ctacgcgatc gcgcccggtc ggcccgaagg catccgggac
    10021 gtcgtctgac aatacttcaa tctctaacgc cgactgctcc atctttagcg cttcgagttc
    10081 ggcagcgact aatcctctca ggcgcttggc cgcttcacgc cgtttccttg taagctgctt
    10141 cgccaggtct gacagtaatt gaaatgccgc gtcgcgtttt gatgtcaact cgctcacgcg
    10201 actctcatga gtctccatcc cctccaactc tgcccgcgct tgagcagcgg tagccaacac
    10261 tgcatcgatc gatccgccat attttttctt gaggcgttga atcaggtcca gccgatcctc
    10321 caactgttcc tgcctatcag gattggcctc caactgatcg gcatattcac gcagccggat
    10381 cgaaagatct tttaactgaa tcagtgcctc tcgcgcctgc tgttcgcact cttgcatcgc
    10441 cggatctgtc tgcttcagct ctccaagatg ccgactcacc cgtccaagcc gcaccagcac
    10501 cccctcctcg tcagcctgca tttgcgcgta cgcttcttca gccaattcac gaagccgatg
    10561 ggcatggacc agccgttgtc gctccgaatg aagtgccgcc tcttctccgt cctgcaagcc
    10621 cgcctgatca atttcctgaa cctggaaccg taacatctct tccagccggt cgcggccagt
    10681 tgccgcctga tgcattgcct cgatctgccc ggaaagatca ttccattccc gataggcgtc
    10741 ctcatatctt gcacgcaacg gcagtatggt tccgaaccca tcgacggcct ccaactggtt
    10801 agcgccagcc aacagcgatt gctgttcatg ctggccatga atatccacaa gggtaccgcc
    10861 caggtcttct agaacgcgaa gtgggcagag actgccgttg atgtagacac gatggcggcc
    10921 ggatcgggac agaatccgcc ggagaatgag ttcgttgtcc ttgggaccaa tgatgtcctg
    10981 agaacgaaga tgctggagca gagaatgggt agcaggaagg tgaaagcgcg cttcgagttg
    11041 ggcctcgtcc tcgccggatc ggatctgttc cgtcgaggct ctcccaccga caagcaatcc
    11101 gatggcatcg ataaggaggg acttcccggc gccggtttct ccggtcaaca cggtaaaccc
    11161 aggctgaaag cgaatgtgaa gctgctcgat cagtgcgaaa ttgacaatgc gcagctcggc
    11221 gagcattgtt gataggtacg ccgttcccag gagagttgac gagaattcac gcccttatga
    11281 cgaacgcgca gccgctttct tatcaggctg acccggcgtg ttgagccaac agctgatcaa
    11341 tcatttcttt gaattgcctc tttggcctgg cgccgacgag tttttccacc acttggccgt
    11401 tcttgaagaa aagaatcgtc ggaatgctca tgacctgata gcgtccggcc acttccggat
    11461 tttcatcggt attgagcttc cgcactttga ctttcccggc atattcattg gccaattcat
    11521 cgataatcgg ggccaccatc tgacagggac cgcaccatac agcccaaaag tccaccatga
    11581 ccagttcagg cgctttgata acttcggcat cccacgttcc atcttcaact ttcaatacat
    11641 tgcctgccac gtcacatcct ccttcgcatc gaacgattca cgttcacaaa atgagcagtt
    11701 atcgtatacc agccctcttc gaggagtcaa ataccggcgc gcgacaacac attcaccatg
    11761 cccattcagg cggcggcgca aaccaccatc catttggatt gatctttcgc catgccgttt
    11821 gctggggcca cacgcgcgcc acaatcgccg catcaagcct cgttgccagc tcggctgtga
    11881 catcggcctg ctgttgcgct gccgactgga tcatttcctt cgcgattcgc gccaagacgt
    11941 tcggtggatc gacaagatcc tccggcgatc ctgacgtcaa gttcagatac agatgttcca
    12001 tcgtaaccca ctgatccatc ttggccaagg attccacata ggcatcgagg gcttggtaga
    12061 cataggtcag atagagatag ctttcggctg aagggtgctc ttggacctct tgccggcacg
    12121 cttcgacagc ccggcgataa tcccctgcgg caagaaaaat cctcgcgcgc tgcaaattgg
    12181 tcggatgggc ttgtgacgcc gagggaggca gcgaactgca cccaaaccac aagcaggtca
    12241 gcatgagcga gcagcacatc gcccggacaa ctgggacttt cgatacatgg atcatcatgg
    12301 aggcccaccg ggaccaggct gcggctggac tgattgcggc ctgccatacg gtccccctgg
    12361 ggccgcccat ggcaatcctc gttctcccca aagcaagggg ctgagaattt ccttcaatac
    12421 gacggttccg tagttctctg tccagccgat tccggtcagg ctgatcatga agttgtactg
    12481 ggctcgatca ggaaattgaa gatagtagag cccgagtgac cagcatttac agggattctg
    12541 ataaagaccc acgatgtcgt attccgggct cctgccgtgc ttcacatcgt agtatccctt
    12601 tgcaccgacc gtccagcccc acggcgtacg gaaggccccc ccgacggtgg taaataaaag
    12661 ctcttcggtc ggagcataga cctcgttgaa tgaaatggga ttccagatat ccccccgccg
    12721 cagccgattg cccgaaatag agtaccgctg cccgacatcc aagtaccaat tgttcgattc
    12781 ctgaacgcgg aagtcggtgt tgaactggct catggtcgaa cgatagggat cataggttgc
    12841 gtcaatcgtc agatactggt tgatgacagg tttgatggca ggagttgctc cggcgccacg
    12901 gccaaacgtc tgccccgcca gttgagattg cgtaatttgc gggctattgt tcccgataac
    12961 agcacgcagc cacagatcgg aaaacttttt tccatccacc gcgaccgtcg ccggctgcaa
    13021 cggctgtgtc acggttccga aggggggagt cactcccggc gcgaaattgc gcgcctggtt
    13081 ctgtaccgcg ccaacgtgat agctctgcgc catcgtcaaa tcgagccaat tgaagctccg
    13141 gtttccgtcc tgctccaata accggcttct cagcatatag gtcatcaaat tcttcttggg
    13201 cagatcgtcg acctgatcga tctgcgtaat ttgttcttga gcgctcggcg ggacatattc
    13261 atacatcaca ctgggctcga tcgtatgaag caaggcattg ccgtcgcccg accaaaaccg
    13321 tcgactcagt ttcgacgtcg catcaatccc cgcccagaac gtttcacgat gctggctgct
    13381 cgtgtcctgc acaccacgcg tataatagac ctcacggaac ttcgcctgcg gggtgattcc
    13441 gacaatatgg ccgaaatcga tgacatccgt ggagaggccg ggaactacat ccacgcgatt
    13501 ttgctggaat ccctgttcac gatagaaatt gacagcactg gcatcccctc ctaacaggag
    13561 cgacgagttg aacagagaca gattcggcag actatagccg agctccggca ggcgctgaaa
    13621 tgtatctact cctcccgatt gcaacggctg cagatattga ctgagcaggt acgtgtttcc
    13681 gtatgccagg cgttgagtcg ccaagagatt cgattcgtta ctgggaaggg ctcgctgggc
    13741 tcccgagttg ctcaactgct gcaggtagtt cgggtcagtc acaaagtttg cttgcgcacg
    13801 cagcaataag tccggtgtga actgttgagt atgggtcccg gtaatcatgg cacgcgcctg
    13861 ctttgcgctt tggcccgtcg gactcacgcc ggacacatcc ggcaactctg tctgctgcaa
    13921 gtaactgacg aaccactgcc cgcgcgattt cttgtccaac acatatcggt actcgaaatc
    13981 gctgccatac ccaagttgtg catagtatga gggagcaatc gtcaggtctt gactcggact
    14041 aaatgcccag taaaaccctt gctgaagatg tgtcccgaag cgattgtcgt acccgacggt
    14101 gggaatgagg agtccgctgc gtcttgagga aagcggatag gtcatcgtgg gaatgggaat
    14161 caccggagca tcggccacgc agagccaccc gccggtaaac gccacgctgt caccagcatt
    14221 gagatccaaa tccttgaatt ggaaccgcca ggccggtatt tccccctcta cggcatcgca
    14281 attggtaaaa cggccctctt tgacccgata gtgatcctct gaaaaccgtt gaatgagacg
    14341 gccttctacg gtggtattcg atgccttgat atacacgtgg ccatgagtga ttacccctgc
    14401 ctcggtgttg acgttgagtt ccaaccgttc ggcagcaaga tctgccttgg gatctgtgag
    14461 ccggacgtgg cctgtggcaa tcatgacacc cggaagcgct tgaacggtca catgatcggc
    14521 ggtgaggtgc aatgctccct gatcaacgac gaccgagcca tccgcctcgt agatctcttg
    14581 atcctgaaga taatcgaggc gatcggcctt gatattgaga ggaaccgcgc ctgaacttgg
    14641 tacgggagcc ggccctgcag atccagcggc ggtcccccag gggggaatca taagtccgat
    14701 tactacaaca caggtcaggc agcccaagat ccgcgtgagg actcctgcca tgtcagcccc
    14761 gaattggcga cagctcacag ccacggaaca gcgccttcac atcaccgatc aacatgagag
    14821 agccggtgat acagatcaga tcgtctgctc ccgccaagcc ccttgcctgc gccaacgcgt
    14881 cgctcagaac ctccttcaca aatgcatgag gccaacagtc tccaatcttc gcccgtaatt
    14941 cctgggccgt cgccgagcgc ggaaggtccg cctgcgtcaa cacaacctcg tcgacgagat
    15001 cgttcaatgg ctcgacaaag ccccgatgat ccttgtcgcg catcatgccc aagaccagaa
    15061 tgatgcgacg cccaggccgg gaaagacgag acgcgcacag gtactcggcc aacgccgccg
    15121 ctgccgccgg gttgtgggct ccatcgagca caaccatcgg ctcgcgatcc acgatttcga
    15181 tgcgaccttc ccagcgaacg ttgctcaaac cttcgcggac tgctttctcg gtgatcgcaa
    15241 ctcctcccga agcggccgat tcaagtaatg ccagtgcgca ggccgcattg tcgatttgat
    15301 gaaggccctg caacgtacag tggaggtcgt cgacctgtag ggtcgatcca gtgaacgaga
    15361 aacactctgg agtccatccc ctgacgcgaa attcacgccc taagcgccgg agcgaagcac
    15421 ccccttccaa cgccactcgt tcaattgtgt ggaacgcctc acccggaaca cgcccgacga
    15481 tcaccggcac ctgcggtttg ataatccccg ctttctcgaa cgccaccgat gacagcgtcg
    15541 tgcctaaata ctcttgatgg tcgagcgcaa tcgtcgtaat cgtgcaggcc agaggtatga
    15601 ccatgttcgt ggcatcgaat cgccctccga gcccgacctc catcacggca atatccacct
    15661 tggcgtcggc aaaatgctgg aaggccatcg ccgtcgtgca ctcaaaaaaa gtaggcgaca
    15721 aatcaggttg acacagtgct ctcaactgct ccgtcaaccg tgcgacagcc gcctctgcaa
    15781 tcatctctcc attgacacga attctctctc ggaactcgat aagatgaggg gaggtataga
    15841 ggcccactcg gtagcctgcc gcttgcagga tcgcagccgt catggcagca gtcgaacctt
    15901 tgccgttcgt tcccgcgata tgcagggtcg ggtagcggat ctgcggcatg tcgagacggc
    15961 tgaccagagc cgtcatcgtc tccaggccaa gtttgatacc gtgcttctgc aggctatata
    16021 ggtaggcgac ggactcggcg taagtcatgg ttggccgggc aggtcgaaaa ctagaaatgc
    16081 gcgacgaggg tgctgagcgt ttccttcagc tgtttacgct cgacaatcat atcgatcatg
    16141 ccgtgttcca agagaaactc cgcacgttga aattgatcgg gcaattgctg tttgatagtt
    16201 tgttcgatga cccgtgggcc ggcaaaaccg atcagtgcct tcggctcggc gataatcacg
    16261 tcccccaaca tcgctacgct cgccgtgacg cctccgaacg tcggatccgc cagcagacaa
    16321 ataaagggaa gcttcgcctc tccgagtttc gccactgccg cagaagtctt ggccatctgc
    16381 atgagagaca gaatcccttc ctgcatccga gctcctccgg aagccgtcac gagcaccacc
    16441 ggcaggcggg cttccagcgc gcggtcgatc gcgcggcata ttttctcccc gaccaccgaa
    16501 cccatgctgc cgcccataaa tccgaagtca aagacgcaga gggcgacccg gcggccgttc
    16561 atctgcccct cgccgatcat gatggcgtct tttctccctg tcttctcttg ctgggccttg
    16621 acccgatctt tgtaggattt tgtatcgtga aaagcgagag gatcctgtga ctcgaggtca
    16681 gcatcccatt ccttgaacgt cccgaaatcc acaagaagcg tgatgcgctc catcaccgag
    16741 atcggaaaat ggtagtcgca cttcggacag accttattgt tccgctccac ttccttgcga
    16801 taaacgatct ctcgacaatg gttgcacttg agccacatcc cctccgcaac cttcgaccgc
    16861 ttcggagcct caggctccga gcctttctgt tttttaaacc acgccatgcg gtgctccttt
    16921 cacggattgt cgggcgttca tctcgatgag aaccttgaga acctcgcgtc gcgacaacca
    16981 gtaaatgcaa gtgcgcaggt taccacaggc cttcctatga cgcacgaaga tttcccctcg
    17041 cagacggtgg gcctcaggac agctgctcgc ccatgaggat gcgatagacg atcgacaggc
    17101 cgagtcccac gcttcccaga ctggctagcc cttgtatggc aaggaaaacg ggtcggccaa
    17161 gctgcaacga ccagacgacc ggcaggctga gagccaaacc gatcagcatc atacccaaaa
    17221 tcgacccgca cccgaatacc atgatgtaaa ccagcccttc catcaccgat cgggcggagg
    17281 acacaaccag aagcaacaat gccgcagacc cggcaagtcc gtgggccatg ccaatacaga
    17341 ggggacgaac cgactcgcgc caccaatggg gatgtccatg atccacgacc accgcatggc
    17401 tgtgtagatg gacgtgcctc gatccatcat gatcatgctg gtgaacatgc caccgttcct
    17461 tgaagagctt caccccaagg agaccgccga ggacaaccag cataaccccg accgcaccct
    17521 cagcgacttg cgccaacaac tccggcactg gcacacggaa ccagagaaca atcaggccga
    17581 cgagcagcaa gatcagcgta tggccgatcc cccaactgaa accgatcaga ccggacgcct
    17641 tcaacgacgg ccgctgggta agaaccgtcg agaccgccgc gacatgatcg ctgtccaagg
    17701 catgccgaag ccccagcatg aagcccaggc tgagcaatgt caggaacgat ggttcgatca
    17761 tgaaatgact cagtcgcagt catcacgaaa ggaatgcttg ggaaaccgcc gcccgaccgt
    17821 ctttccggtg acgccgctga gaccgaagtt caccggggcc cctcgaaatc cagggcacca
    17881 tgggcgtcga gtcagcgata cgtcgagaag agcgagtgag aaaaaccccg ccggacgaga
    17941 ggatcggccg ccgatttcag ccgccgcgag cgtgcatttc gagatgcgga tcttcacgaa
    18001 gtctctcgat gccgatgagt ccgccttctc ggagccccac gcgctcgagt gccttgcgtt
    18061 cctccgcacc acgatccctc ctggcttccc acccagcacg gatgaaggct cgcatatcgt
    18121 ccgccgacat cccgttgcga agcggtttcc tcaagtctgt tccggttgtc gcatacaaac
    18181 agcgatacca catgccgtcg gccgtgacac ggctccggtc gcaggttgaa cagaacggcg
    18241 tcgtcgtcga gggaatgatg ccaaaggtcg tgccgtccga taagaggaat cgctgtgccg
    18301 gcgcagaccc acgctccggg atcggctcaa tctttccata gtacttgcca agcgtggtca
    18361 acatctcctc gcgcgacagc actttttcag gagcccactc gtttgcgccg ccgacatcca
    18421 tatattcgat gaaccgcacc tccgcctgat aatgcttgcc gaattcgatt aaggcaacca
    18481 gctcgtcgtc attgaatcca cgaatcgcca ccgtatcgag tttgaggccc gtgaatccag
    18541 cgcgaccgac ggattcaatc ccttcaagca cccgtgcata ctcgtcgcga cgcgtcaatt
    18601 gacgaaaccg ctccggccgc aaggtatcaa gactcacggt cacgcgatgc aaccctgctt
    18661 cgtacaaggc ttcgatctga tcactgagca aggtgccgtt cgtcgtgagc gcaatctccg
    18721 taatccgtcg attttggagc aggagccgaa tgagccgggg aacatctcgc cggagcaggg
    18781 gttcgccgcc ggtcaacctg actttgtcga cgcccagatc ggtgaaatag cccgtgagct
    18841 ccgccatctc ctcgaacgtc agcacatcct cgcgcggcag ccagatgtaa tcttcttccg
    18901 gcatacagta cgcgcagcgc agattacagc gatccgtcac tgataagcgc aaactccgta
    18961 gtggccgacc ataggtgtca accaccgtcg ggagcgagtg actgtcaatc gcttcgttca
    19021 ttcttacacc aaaggcccgc gcttacggat gctcttccac ccacacctcg ccactgggcg
    19081 tatgttctaa cttccaaatc ggtgtaatct gtttcaactc atcgatcgcc catttgcagg
    19141 cccggaaagc ctcagcccga tgttccgcac cggcgataat cagaacgatg ttctcgccga
    19201 ttgcaatttc gccatagcga tgaacgatca gcaattccag aatgtcgaaa tccttcaacg
    19261 cccgctcgcg aatctcgcag agcttcttct gcgccatccc ctcgtaatgt tcgaaggtaa
    19321 tgctgtcgac atcccgtccc ttcgaccgat cacgcgcagt accaaggaaa atcgaaatgc
    19381 ccccaatccg ctttgaccgg ctacgcacac ggttgatctc cgcatccacc gaaaagtctt
    19441 cccgttgcac acggactaac tggctttcat caagcgtttg ttccataagt gaaccgcctg
    19501 caaatggagg taagagcgcg atctcatcgc cttcgcgcac ttctgtatct tcatgcgcaa
    19561 tttcctgatt gactgagacc aggatttttt tcttgtgaat caactcgccg atcatgggat
    19621 agccttcatt gaccaatccc actaaatcct tgacgcgctg accgttcgcc agcgccagcg
    19681 agagtgacac ttgattcccc gccagcgatt ttgtcatgcc gaataatctg actgtgatca
    19741 tctttccctc aacctcagcc ttctcttgtg taggttccgg acttgccgcc ggatttcgac
    19801 agcaaacaga tgtcactgaa gctcattccg cggtcgattg ccttgcacat gtcgtagatc
    19861 gtcaatgccg cgactgaggc ggccgtcatg gcttccatct cgacccccgt ctgaccggtc
    19921 gtcttggccg tcgccgtcac gataatcgca cagcgaccat tgcgatccgg caggggttct
    19981 tctgtaaagg agatatcgac ggaggagaga aacagagggt ggcacatagg aatgaggtcc
    20041 ggagtcttct tggctcccat cacccctgcc acttgcgcaa cagccagcac atcgcccttg
    20101 gcgattttac cccgttgaat cttttcgaga gtttccggct ggagataaac cttggcttga
    20161 gctgtcgcca cccgctcgga ccgggctttg gcgctcacat cgaccatccg agcccgcccc
    20221 gactcattga agtgtgtgaa ctcagccatt tttcctcgct gaatcagccg gttatgcttg
    20281 cggccatcgg tgattatagg tggcggtgaa atggcgagtc aagggtggct atagacgagg
    20341 tcgtagcaaa aagccgagac cagccacggc actgccatga ctgatagctg cggtggaaat
    20401 tctggaggga ttacgcgagg tcatgccgat ggcggacacc ctggaaaaca ccagaactga
    20461 ccagctgccc actctggtca ccccgccaac gtctggttga cagtccggat tgccatcccg
    20521 gacaatggcc cacatgacag gcaagatcgt tatcgaacga ctggagtttc aggggcggtg
    20581 cggcattacg cctgaggaac gccttcgccc gcaaccggtg gctgtcgatc tggaattgga
    20641 ttgcgatacg gcgctggctg gcgcatccga ccgcatttcg gacacagtcg actatgccca
    20701 agtcgcggaa cggatcgtgg aactgggtgc aacccaagac tgcgcgctgc ttgaggcgtt
    20761 ggccgagaac ttgctgacga tgctcttcac cgacttccct gtggaacgtg tcaagctctg
    20821 gcttcggaaa ctggctcccc ctcttaccca gatgaccgga tcggtgggaa tcaaagtgga
    20881 gcgcgcccgc atggcgcatc acgcgcaaca aaccgatccc tctccttctc gttttctcac
    20941 ccaacaactc catcgcttgc cgaaaggccg cgtgctcgat gtcgcttgtg ggagcggacg
    21001 ccacactctc tatctcgcca gtcacggatt tgacgtcgag gcaatcgatc gagatcccga
    21061 caacctgacg aaattagcct ccgcagctaa acaacggcat ctttcaaacg tcaccgtgag
    21121 gcaggttgac ctcgaacgcg ccaccgacga acggccagaa tttccggcca atcaatacga
    21181 tgccatagtg gtctgcttct acctccatcg tcccctgttt ccttggctca tagaagcctt
    21241 gaagccacat ggggtcttgc tctatgaaac ctttacgatc gacaactatt ttcgccatcg
    21301 gcacccgcgg cggtgggaat tctgcctcgc acacaatgag ctgctgcgtc tgacgtcctc
    21361 gctccgcgtt ctctcttacg acgaaggaga acatgagggt ggaccaggca tgggctcagt
    21421 cttcacggcg caactcattg cgcaaaaacc gggcccggcc ggaagcctgc atgagccgca
    21481 ttgatctcca cctccatacg acccattctg acggaagcct gcgtccttcc gatgtgctgg
    21541 cgcttgcaag acaggcgaac gtcagcgcgt tagccatcac cgatcacgac atcacgacgg
    21601 ggatccctga agccatggcg accggcgaaa ctctcggcat cgaggtcatt cccggtgtgg
    21661 aaatcagctc gtttgatggg aaaagcgaac tgcacatcct cggctattgc gtgcgatggc
    21721 aggatgccgc atttaaccag cgtcttgcta cgctgcgcga gagccgccat cgccgcaatc
    21781 ccctgattat cgagcggctg cgcgaggcgg gtctcgacgt caactatgag gaagtccgcg
    21841 cgctcgcagg caccgaatcg gtcggacgtc cccatattgc ccaactgttg atgcagaaga
    21901 agtacgtctc ctcagcgaaa gaagcctttg atcgctatct tgcggaaggc agaccggcct
    21961 atgtggcgcg cgagttgccg acaccggcgg aagccatcag ctggattaga gaagccaaag
    22021 gcgttgctgt tctggctcat cccacctggg tgaaagagtc cggtgaggga ctgcgcacat
    22081 gcgtgacaac cctcaaagaa gccgggttgg gcggcatcga agtccactat agtacgcaca
    22141 gcaagtctca gacggcaggc tacctcgacc tggcccaccg tctcgactta ttgatcactg
    22201 gcggcagcga ttttcacggc ctcaccaagc ccgatatcga ggtcggtagc ggacgaggtg
    22261 acctccaagt aaaccctcgc ctgcttgctc ccctcaaaga agcggccgca cgcatttaat
    22321 acccttccgc ttaccgcagt tttcccccaa ttcgttgact ctccgatgcc atccgttaca
    22381 ctgaggacca ttcaccttct ctctcacaaa gggtatgtcg aaacacttac cttgggtcct
    22441 cccatatggt gccggtatcc ttgccgccct tctggtcagc ccgctgctca gcagcatggc
    22501 gtgggttgag ttcttcagca ttcctctcac gccagtaaca ggccctgacg ccctccggtt
    22561 gacggcggac gcgattgtgc tggggctggt cctgtctctc gccgtcacag cccaacaacg
    22621 gctctctgat aacggccgcg gctcgggttt tctccgcgaa atcctgttcc cactggccac
    22681 gttgcttgtg gtcatctttg gaggcaaagc gcttcgcacg gcagggtttc cgctcgtgca
    22741 acagattggc ccctcccctt tttcctgggg ttatgctctc gcgctccttg gatcggggct
    22801 ttggacgacc gtcgcttggc tccgccatca ggacatgctg cgcaacgcct ttacgccccc
    22861 gccagctgca agacgattga ccaagcatac agaacaggta gaaacacatg aaccctcgga
    22921 cagcagcaac gaactcgcca ctcagccgaa ggaaaaagca ccggccgtca ctgcaaatgg
    22981 aacccctcct gcgactttgg gccgctataa gatcctgaaa gaactcggac gcggcgcaat
    23041 gggtctcgtt tacttgggga aagacccgac aatccaacgc ttcgtggcaa tcaagaccat
    23101 gcgtctcgat caaatcgacg aagagaagaa actgcaagag aacaagtctc gctttttccg
    23161 cgaagccgaa tcggcaggcc gcctgtccca tcccaatatc gtgaccattt acgatgcggg
    23221 cgaacaagac gagctggggt atatcgcgat ggagctggtc gaagggaaat cgttgaagga
    23281 gtggtctcgc aagccgaatc tcatgccggt cgcagaggtg gtgcagaact tgagcacggt
    23341 ggcggatgcc ttggattacg cccatcagca gggcgtggtc caccgcgacg ttaaaccggc
    23401 caatatcatg atcaccaagg accggctgct gaaggtcatg gatttcggca tcgccaaggt
    23461 ggtctcaagc agtaagaccc agaccgatgt cgtgctggga acccctacct acatgtcacc
    23521 ggaacagatc gccgggaaaa aggtcgatgg acgttcggat atcttttctc tcggtatcgt
    23581 gttattcgaa ctcctgaccg gacaacctcc gtatacggcg gacaatctct cggccttgct
    23641 tttcgcgatc gcccaccaac cgcatcccca gcttcatgcg gtccgccagg atcttccgcc
    23701 ggtgttgcag gaggtgctca atcgcgctct ccaaaaggaa ctgccgcagc gctatcgccg
    23761 cgccggggaa ttcgctcaag acctccgtgc ctgtctttgc agcctcgcgg cataacacgg
    23821 agaacagggt ggcaatcgaa gctctccaca gcgccaagac cgacatcggg cgcaagcgtc
    23881 ctcacaatga agactgcttc gtcggcgacc aagaactcgg tctttacgtc gtctgcgatg
    23941 gcatgggagg cgggaacgcc ggagaagtgg ccagccgcat ggcaatcgac acgatcatgg
    24001 ctcacgtgaa agcggctata gaggaccccg ccgtgcaact gatcggcccg gtcgatccca
    24061 atctctcacc agccaccaac caacttgcca gtgccatccg cgccgccaac gaggccatcc
    24121 atcgagcctc ttggaccaat tcgaagtacg ccgggatggg caccaccgtc gtcgcggctc
    24181 gtcttacaga acagcatcta tccatcgccc atgtgggcga cagccgtctg tatctaattc
    24241 gagaccacat tcttcatgcg ctgacgaccg accattcctg ggtggcagaa cagatcgcac
    24301 agggctacct gacggaagag gaggcggaac ggtcgccgcg acggaatatc gtcactcgag
    24361 cgctgggggt cgaaggcagc gtggagattg atttgactga gattccgatc ctcaacggcg
    24421 acatgctgtt gttatgctcg gacgggctga cgcgcggggt tcgctccggc gacattctgc
    24481 agacactcaa tatccccact gatctcggcg ccaaggcgga ccggctggtc tctctggcga
    24541 atcaggccgg aggggatgac aacatcacgg tcctgctcgt ggcaattcaa cgcggggcga
    24601 ctgatcggtt atggcatcgc attcggcaac gttggtttct gaaagcatct taatcttcac
    24661 aggagagtgc attatggcgc ataccgtcac cgaatccgca acactcctca tcaaaatcaa
    24721 ggggaaaagc tctcggacaa tcgacatcgc accagagacg ttcaccattg gacggaaggc
    24781 tgataacaat ctcccgatcg acgaccatac cgtctcgggt caccatgcca agattgtcag
    24841 agtgcagtca gtctatttcc tggaagatct caagagtacc aatggaacgt tcctgaacgg
    24901 gaaaccgatc gatcgggctc agcttcatga cgccgacgtg attacgatcg gccaacatcg
    24961 aatcattttc caggacagca ctatcacccc tgcagccacg tcatcccagc cggtggatct
    25021 ggaacaaacg atggcaatca gcagcaagga ttcgcatgca aagccgccaa cgacgacagc
    25081 caaagtgctc gtgaccgccg gcaagaccga gcggctggaa tatcacctca ccaagcccgc
    25141 caatctgatc gggtcgcagg agggtgccgc gattcggctg accgggtggt tcgcgcccaa
    25201 gtcggcagca ctcgtctcca accgaggcag catctatgcc atcagtcctt cacaggggat
    25261 gaagaaacta caggtcaatg gcgagaacgt gtcgggtcag cggcagttga acaacggaga
    25321 tatcatcgag gtggcgggcg tctcgatgac cttctatctg gttcctccga aagggaagta
    25381 aggtctagcg aggggactac cctactgttt gaaatacaca gtgcccttgg gaagggtttc
    25441 gtagcaacga tcggatactt cctgcgcatg atcctgaaga cattgtaaga tctgcccgtc
    25501 ccccggcttc attgacgcgc accaacgttt cacatcggcc tggcaggccg tcaccaaccg
    25561 ggtgcgctct tctttccact tcacaaaccg ctcgcgaagc tgttgttgac agggtcctgc
    25621 cagttgctgc gcgcgcttct ctagacatct ccttcgtgcg ggaccttcca gggagtccgg
    25681 gcacaattgc tcaatttctg cttcacatcg gagacgggcg acttgcacct cccgagggtt
    25741 gagctgcgcc aggggtgata acgacgcccc gacagttgcg gccggattct gctcggaagg
    25801 agccggcgga acagaaggca cacctgggat cgaaagatcc aacgtcggct cggaagggag
    25861 ttgagcctta gaagctgaaa ttggtagctg cgatagcttc ggttctttcg cggaaagttc
    25921 ctgttgcgat ggaaggctca tgaggatggc cagccagaca atgccaagtc cgacaaccgt
    25981 aacaacccca gcgacgatac ggccagacga atgtgcttgc cccatgattc ctccttacaa
    26041 ttccagccca caggataggg caaggtcccc caaaaatcga cggaatacga ggaattcgca
    26101 ggcagtcgca ctccttgagc atcaaagagt tctgtgggta gggacggacg agaaatggag
    26161 gaaggattca gttttccgga gcttcgacaa tatgattttc gaacctggtg cagccttcgg
    26221 ccagcaatcg atttgtcaac atctctcccg gccactcaat cggcagatct gcggtgaaga
    26281 cccagacgtc gggtgaagtc cagaccggac catgctcatc cggcaattca ggatcgaaca
    26341 gatcggtcca caccggcata tagactccgt tcccgcattg ggtatggaga tgaacgatcg
    26401 tccgagcacg tacgagtggg tcgagatctc gccacacagc cgccgtctcg tcggccagac
    26461 gatggagcct gacagcattt tgcgcgtcga acatggcgta ggcggcatag accggagctt
    26521 cgagggtatc cggcgccaag gccagttcag gacattgctc caccaatcct tccaagagcg
    26581 cccggcgatg cggatcttcg agcgaatcgg gcaaacccga atccggcaca ccgatcaact
    26641 caaagaagag aaacgcggta ttctcgacgg gcggatatag acgggcagca atcgaacggg
    26701 cccgctggga atcggcaacg gtgccgagat gttcgtttag cgcttgcaga gtcaacgcct
    26761 cgagtgtggc gtcggttaac agtcgtggct ctacgcgaac gacggttccg gccgacaacc
    26821 ggaggtgcct gtgcaggatg tctgcggttg ccaccggatc gatcttcaat ttcaagggat
    26881 gggtgagatt cgcctgcaac ggaagttcca gcgccgccat aacggcatac gacagcttct
    26941 catcgaccgg gccatccagc aggatggaac aggtggcttc cgcaaccaaa tcgaacaacc
    27001 actccgccat gtcttcatcg agcgcggcca aaaccgtcaa gaggtcgtgt tctcgtccct
    27061 gttcgagaaa ggattgcagc gtgtcg
//
LOCUS       DATIFR010000141        12478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_669382, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000141 DATIFR010000000
VERSION     DATIFR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669382"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..230)
                     /locus_tag="VLD60_08615"
     CDS             complement(48..230)
                     /locus_tag="VLD60_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45060.1"
                     /translation="MSGVYWGIVGGFLAMVATLFVCFDLLYPKAKGSPTARSGRIDEP
                     SEAGTQALDGRRRAAA"
     gene            complement(278..1231)
                     /locus_tag="VLD60_08620"
     CDS             complement(278..1231)
                     /locus_tag="VLD60_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45061.1"
                     /translation="MSYRYNTTDIIVGVGMCAIVFGALLFFVAANGTYQIAIPQPSSL
                     EEPAGIDVGMSWLQPALGQAIVDQVVFERRANQVIAQSASEWNRATIAHHEFQSRSGG
                     PFGAIMRQAATVPADHMARVQGVMGRAIVNFTQRGIRSGVLSADQYLSDYNSGMIRAT
                     EARGQRLDQNFASTWQATLGRGIVEAVQSYATKAGAIQERMGRALFHLTQAQRSSEEE
                     RAAQQEQFASLIVADIRTEALADRLTLLAAIQSFPEDTAVAFTEPASWPEIPMGYLMV
                     AGFMLAAVFFGGLSLSARSRETKVVDQMKQTANRWVYRVAA"
     gene            complement(1818..3458)
                     /locus_tag="VLD60_08625"
     CDS             complement(1818..3458)
                     /locus_tag="VLD60_08625"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HSB45062.1"
                     /translation="MKAAELLVRCLENEGVELIFGLPGEETLELMDALLGSRMRFIET
                     RHEQGAAFMADVYGRLSGKAGVCLSTLGPGATNLLTGVADAYLDRAPLVAISGQASLN
                     RRHKESHQSIDVMSMFKPVTKWSTSIPKAEVIPEAVRKAFKIAQTEKPGATHLELPED
                     VAEEQIGDPRTLQPLFVQSPVMPEPSPAQVARAVRAISGAKRPVILAGNGVIRGRAYE
                     AVSQFARRLHIPVLQTFMAKGTLPDSDPLSLYTIGLQARDYTTMVMEQADVVIAIGYD
                     FVEYAPCFWNARRDKCIVHIDGSPAEVDEHYIVEVGVLGDLRLSLDHIGTEVAPFDSL
                     WASGSRKAVIEGFEAELAGASSWPIRPQHVMRELRAALKPDDLVICDVGAHKLWMARM
                     FPCEVPNSCIISNGFAAMGIAVPGAVAAKLLFPERRVVAVTGDGGFLMNSQELETAVR
                     LKLPLVILVWRDNGYGVIRWKQQLRFGRTSSVEFGNPDLMRYAQSFGAIGYRVTEPSE
                     LRPVLVEALNSKVPAVIDCPVDYAENLRLTERLQSLPH"
     gene            complement(3743..3970)
                     /locus_tag="VLD60_08630"
     CDS             complement(3743..3970)
                     /locus_tag="VLD60_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45063.1"
                     /translation="MKSEKKKTTQKEGEGNTTGTPPFEKPGHCLICGQQGPTLLYGHW
                     ICDHCKNTVLAEAIAKKRRIEKEGGGPVPAR"
     gene            complement(4211..4780)
                     /locus_tag="VLD60_08635"
     CDS             complement(4211..4780)
                     /locus_tag="VLD60_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45064.1"
                     /translation="MTAESYGKDKTKGLVVVSAMWGRTWKCAQFESGQLRSFGFDRLP
                     SQTTADDATADVVIESSPYYGEGPAKNYVLALDPGEYGLATFTMNVASSPTDVKTASV
                     GRARLFEGRKPIAGTFNVKAGELVYIGHFGLDCYKEPTIWRYYPEGREGFDNYKQVIK
                     KQYPFLNVDQMQFRLFKTSLLGRDYGLPQ"
     gene            complement(5115..6191)
                     /locus_tag="VLD60_08640"
     CDS             complement(5115..6191)
                     /locus_tag="VLD60_08640"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HSB45065.1"
                     /translation="MTIVQATRSYEMQLATQTSLVSPDLLLKHKLMRNDEFAFFRATF
                     YRWMQCWPTVCSDLTTAPVVLAVGDLHVENFGTWLDVEGRLIWGINDFDEAYPLPYTN
                     DLVRLAVSAKLAIMTEFLTIKPKDACDAILSGYTEGLKAEGRPFVLAERHQWLRDIAT
                     GKLRDSAGFWRRIKDLPPVKGAVPPSARAALEHLMPEPGLPYAIRRRISGVGSLGRPR
                     YVAVVDWCGGKIVREAKALVPSACVWAHDGHGTDAIQYQSVLDDAVRCRDPFVHVDGS
                     WIVRRLSPSCSRIELASLPKKRDEARLLHAMGWETANIHLGSRKMHKDLKRDLANRNA
                     DWLRVATKTMTKATLSDWKDWVRK"
     gene            complement(6384..6782)
                     /locus_tag="VLD60_08645"
     CDS             complement(6384..6782)
                     /locus_tag="VLD60_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSB45066.1"
                     /translation="MVSVLRISTTNRKQVVDLTDRIETVIRRAKMQEGLCSIFITHTT
                     AALTTGEIGEGTEDDFLQVVEQMIPRIHFRHAHDPSHAWSHMASSILGPSLTIPVSAG
                     KLVLGTWQSVMLVELDGPRERDVHVTLVPS"
     gene            7107..8651
                     /gene="hpnR"
                     /locus_tag="VLD60_08650"
     CDS             7107..8651
                     /gene="hpnR"
                     /locus_tag="VLD60_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HSB45067.1"
                     /translation="MNLLLIHPSPLMYTKIYLRLEPLGLELVAAAARRLGHNVTLLDL
                     QVESHEQFIRLLRERRPDAIGFSCNYLANVPEIIDLSKLAKQHLPHSVVFVGGHSASF
                     VAHAILDHADGAIDCVLKGEGESTIGQLLNVIEEDRTALNRIPGSVTLNGEGPPAQMV
                     TSLDDLLPARDLLRHRRRYFIGVLDPCASIEFTRGCPWDCSFCSAWTFYGRSYRVASP
                     EAIMEDLSRIREPGVFIVDDVAFIQGKHAMAIGEAIARHGIKKQYYLETRGDVLLRNK
                     EVFQFWKTLGLTYMFIGIEAIDAEGLALHRKRMTISKNFEALEYARSLGITVAINLIA
                     DPSWDRRRFEIVRQWCLEIPEIVNISVNTPYPGTETWHTETRPITSRDYRLYDIQHAV
                     LPTTLPLPDFYEELVKTQQILNRKHLGWTALKSTARIAADHLLHGQTNFVKMLWKFNS
                     VYNPQLQLADHAKPVTYQMPLPQKRHQGLDNHLLYIHRSQGREGRLLDDATERFVEAT
                     RSSAAN"
     gene            complement(8763..9176)
                     /locus_tag="VLD60_08655"
     CDS             complement(8763..9176)
                     /locus_tag="VLD60_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45068.1"
                     /translation="MADLIRAAQYFKVTIADKSGTLAHMLAPLQDAGVSLLAVHAFPR
                     NRRTQVDVVPEDATGFKDATKSLKWKVQGPKVCFLVEGDDRPGALVEVTSQLGLAKIN
                     LTAVTAVVAGQGRFGAILWVKTKDVKKAAKLLGIE"
     gene            10040..10597
                     /locus_tag="VLD60_08660"
     CDS             10040..10597
                     /locus_tag="VLD60_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB45069.1"
                     /translation="MATITKSDKFLPGEEQTSATRTPPKKILVTVDDSDQSVRAMHYV
                     GSLLRDTHDVGVTLFHVLKPMPRELMEHGGSEIPEIEDHLGTQLRKDQEDWVRMEGAI
                     EYPILLKALERLGQTGFPIEQVTLKFGHERDIADTIIDEARSGGYGTIVVTRQETSSV
                     KRLFGNGIIDRLLRDVSGVAIWVLS"
     gene            10817..11608
                     /locus_tag="VLD60_08665"
     CDS             10817..11608
                     /locus_tag="VLD60_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB45070.1"
                     /translation="MDCTRCEATLPLKARFCPSCGSKVTLWEELMLDEPVSRSPESAE
                     EHGERTSPLPRDNEELRARLIECYLNFSISKELSEWLQDLGLPSTGTTQEKLAGLRRH
                     AGSLVLPAESFPRQTIYYLNKYDEEVLSEICQELGIDSTGPKETLLTRIYREVGLREG
                     WIQPLSEDARRIITETFVPILKSFDLKKDYYLDLWGELSDVLGEDNVRLHVPQAYGSA
                     IIAVLIPGYFQEAQMTMLQNELKDRAEKRSSQRAAPVVESVTGQG"
     gene            complement(11644..12066)
                     /locus_tag="VLD60_08670"
     CDS             complement(11644..12066)
                     /locus_tag="VLD60_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB45071.1"
                     /translation="MAWSIASKIVRRVVTIDGSHSVLEAATLMAEEFVGSAVITSSSK
                     ITGIFTERDLMMRVVGKKRDPDTVKINDVMTKKVVIVSPKDTANYCLNLMKEHRCRHL
                     LVFDSDEFIGIVSLRDIVTLMIEEKEDLIARLQQYITS"
BASE COUNT         2800 a         3738 c         3312 g         2628 t
ORIGIN      
        1 cacctccctt gtctctataa tgagactcgt cgaggcctac cgcgcaccta tgcagccgcc
       61 cgacgacggc cgtctaaggc ttgcgtgcct gcctcgctcg gttcatcaat cctgccactc
      121 cgtgctgtgg gcgacccctt cgccttcggg tacaggagat cgaaacagac gaacaaggtc
      181 gccaccattg ccaggaaacc tcccacgatt ccccaataga cgcctgacat aaatcactca
      241 ctcgcgcgca ccgcgcgagt ccttttctca catgcggtta cgccgctacg cgatacaccc
      301 acctgtttgc ggtctgcttc atctgatcga ccactttagt ttcccggctc cgggcgctga
      361 gcgaaagtcc tccaaaaaag actgccgcca acatgaaacc ggcgaccatc agatagccca
      421 taggaatctc aggccaggac gctggttccg tgaaagccac tgccgtgtct tctggaaaag
      481 attggatggc ggccaatagc gtcaagcgat ccgccagcgc ctcggtacga atgtcggcca
      541 caatgaggct cgcgaattgt tcctgctgtg cagcccgctc ttcttctgac gatcgttggg
      601 cctgcgttag atgaaaaagc gccctcccca ttcgctcctg gattgcgcca gctttcgtgg
      661 cgtagctttg gacggcctcc acaatccctc gtccgagggt cgcctgccat gtggacgcga
      721 aattctggtc gagacgttgc cctctggctt cggtcgcccg aatcatgccg ctgttgtaat
      781 ctgaaagata ctgatcagcg gacaatacgc cacttcgaat gccgcgttgt gtgaaattca
      841 cgatcgcccg ccccataacg ccctggacac gggccatatg atcggccggg actgtcgcag
      901 cctggcgcat gatggcgccg aagggaccgc ccgatcgcga ttggaactcg tggtgagcaa
      961 tggtcgcccg gttccactct gacgcggatt gggcgatgac ctgattggcg cggcgttcga
     1021 agaccacctg gtcgacaatg gcttggccaa gggcgggttg gagccaggac attccgacgt
     1081 cgatgccggc tggttcttca agcgagctgg gctgcgggat ggctatctga taggtgccgt
     1141 tcgcagcgac aaagaacagt aaggctccga agacaatcgc acacatgccg acacccacga
     1201 tgatgtcggt cgtgttgtag cggtacgaca taacgacctc ctttcgacat cgggcaagca
     1261 ggcgcgtggc aagcacgaca cgcggcatct tgcgcgagtc acatgaaacg atttccggtt
     1321 cctgcagttg ggcatagtga tagacctccc tgtgtgacac ggaacgcgag accacaaggt
     1381 cgcagaggct cgtccggtgt tcaccagacc agctgaaaat ggcaagtgag cgatgttgca
     1441 ggaaagaatc ctgatggggc ggaggccgca ctcgccatgt ccattccgtt ggatctgtgt
     1501 accacggagg aacatggcta ccacctccag gatgaggcgg ctcgtgaaca agttttaact
     1561 ttacgctccg gcgaggcacc ggtcaagagc agaaatcgca agtcccgtgc ctctttcttg
     1621 ctcgagctta cgtcagcaat ggcgcttcga aaacactgat ccataagctc gttaagtcga
     1681 tctcgaactg tctctcaaac gttgtgaaga tcggatgcgt accgaagaag atacgtggct
     1741 ggatcgaatt tgattctaga tgtgcggacg agatcggcga gatagatcca gacaggatct
     1801 ttcacgcaaa cagcgtgtca gtgtgggagc gactgcaggc gttccgtcag tcggagattc
     1861 tcagcgtagt caaccggaca gtcaatgacc gccggcacct tgctgttcag cgcttcaacg
     1921 aggacgggcc gcagctcgga cggctccgtc acgcgatagc cgatcgcgcc aaaactttgc
     1981 gcatagcgca tgagatcagg gttgccgaat tccaccgagg aagtccgacc gaaccggagc
     2041 tgttgcttcc agcggatgac gccgtagccg ttgtcccgcc aaaccaaaat gaccagcggc
     2101 aatttcaaac gcactgccgt ttccaattcc tgggagttca tcaaaaaccc gccatctccg
     2161 gtcacagcca ccaccctccg ttcaggaaac aacaattttg cggcaactgc gccgggcacg
     2221 gcgatgccca ttgcggcaaa cccgttcgag atgatgcagg agttcggcac ctcgcaggga
     2281 aacatcctcg ccatccagag cttatgggcg ccgacatcgc agatcacgag gtcatccggc
     2341 ttcagagccg cgcggagttc ccgcatcacg tgctgcggcc gaatgggcca ggacgatgcg
     2401 ccggcgagtt cggcttcgaa cccctcgatc accgccttgc gggagccaga tgcccagagc
     2461 gaatcgaacg gcgcgacctc cgtgccgatg tggtccaacg agagacggag gtcacccagc
     2521 acgcccacct cgacgatgta gtgctcatcc acctcggccg gcgacccatc gatgtgcacg
     2581 atgcacttgt cacggcgtgc attccagaag cacggcgcat actcgacaaa atcataaccg
     2641 atcgcgatga ccacatcggc ttgttccatc accatcgtcg tataatctcg agcctgaagg
     2701 ccgatcgtat agagagaaag cggatcgctg tccggaagcg tccctttggc catgaacgtc
     2761 tgcaggacgg gaatgtgcaa gcgccgcgcg aactggctga ccgcctcgta ggcgcggccg
     2821 cgaatcacgc cgttcccagc caggatgaca ggacgctttg ccccggaaat cgcgcggact
     2881 gccctcgcga cctgggccgg cgacggctca ggcatgaccg gcgactggac aaacagcggc
     2941 tgcaacgtgc gcgggtcgcc gatctgttct tccgccacat cctccggcag ttccagatgg
     3001 gtcgcccccg gtttctccgt ctgggcaatc ttaaacgcct tccggactgc ttcgggaatg
     3061 acctccgcct tcggaatcga ggtactccat ttggtgacgg gcttgaacat cgacatgacg
     3121 tcgatggact ggtgcgattc tttgtggcgg cggttcagcg acgcttgccc tgagatggcc
     3181 acgagcggcg ctcgatccag gtaggcatcc gcaacgccgg tcaacagatt ggtggcgccg
     3241 ggtcccagcg tggacagaca cacccccgcc ttcccgctga gtctgccgta gacatcggcc
     3301 ataaacgccg caccctgttc gtgccgcgtc tcgatgaagc gcatgcggga accgagcagc
     3361 gcatccatca actccagcgt ctcctcgccg ggcagaccaa agatcagttc aacgccttcg
     3421 ttctcaaggc aacggaccag caactctgcc gccttcatgc gaagcactcc tcaatgggga
     3481 aaacgcatag accgatgcca gaccagagcg acggcccgga caatcggcag atctcgcata
     3541 tgcattattc tatcaacctt gggcgggatg gaaaaccgaa ccgccctgcc aggaggaggg
     3601 gatcgggaaa ggagaatttc tccatttttg actcagaata cctttggaag cggagcctcg
     3661 aacacgggcg acttctctgc ggaatggatg gtcagcaaat gagaaccaca ctgtccgact
     3721 ctgtaagtga atcgcaatac ttctatcgag cgggaacagg cccgcccccc tctttttcaa
     3781 tccggcgttt cttggcgatg gcctccgcca gcacggtgtt cttgcaatgg tcgcagatcc
     3841 aatgtccata taacagagtt ggtccttgtt gtccgcagat cagacagtga ccgggctttt
     3901 cgaacggcgg tgtccctgtt gtgttcccct ccccttcctt ttgagttgtt ttctttttct
     3961 ccgacttcac aatggccctc cctcgttgct gaagtgacaa ccattcatct gcgagtatcc
     4021 cctgtctccg attagcctcc ggttcttgga tcccatcagg tagcgaagct caactgaccc
     4081 cgagagaagt ccgggcgaag acaaaaaggc cacgccgcgc gcaggcgagg caaaactgtt
     4141 cgtgacccgt tctcctcaat tcgaatactc tgaagatcaa gaacgcacga tcagcatctg
     4201 aatctagctc ttattgtggc agtccatagt cccgcccgag tagcgatgtc ttaaataatc
     4261 gaaactgcat ttgatccaca ttcagaaatg gatattgctt tttgatcacc tgcttgtagt
     4321 tgtcgaaccc ttcacgccct tccggatagt agcgccaaat cgtcggctcc ttatagcagt
     4381 ccaatccgaa atgcccaatg tataccaact cacccgcctt cacattgaac gtcccggcaa
     4441 tcggcttgcg tccttcgaag agtctcgcac ggccaacgct cgcggtcttc acatcagtcg
     4501 gtgatgatgc cacattcata gtgaacgtcg ccaagccata ctcgccagga tccagcgcca
     4561 gcacgtaatt cttggccggt ccttctccat aatagggaga gctttctatg accacatcgg
     4621 ccgtagcatc gtctgccgtc gtttgactcg gcaaccgatc gaagccgaag gatcgcaact
     4681 gtccgctctc gaattgcgca cacttccacg tacggcccca catcgccgat acgacaacca
     4741 gtcctttggt tttatctttt ccataagact ctgcagtcac ctgctgcttg ggccttgaga
     4801 caccagatga cgtacagcca gacacgagaa cgattagtat cgtgagaagc gcgaaacctc
     4861 taagcattga gtgtggctcc aacctatcgc ctccgccgtt cagccattca gccctaccat
     4921 agggcggacc ctacgctccg aacgagcgga cgtcaacaat gcgcggtcca gccggtgaga
     4981 tcaggcgaac gaggtgggcg gctggcgggg gtcgagacgg ctattcagat cgaaatggtt
     5041 ccagacgctt cttcatttcc agagaccgcc gcgcacggac gaggcgcata cggtttctgg
     5101 caccgttgtc tattctattt tcgcacccaa tctttccagt cgctgagcgt cgctttcgtc
     5161 atcgtcttgg tcgccacacg aagccaatcc gcgtttcggt tcgccagatc ccgtttcaag
     5221 tctttgtgca tcttgcggct ccccagatgg atgttggccg tttcccagcc catcgcatga
     5281 agcaggcggg cctcgtcccg tttcttcggc agagaagcca gctcaatccg cgagcaggac
     5341 ggcgagaggc ggcgcacaat ccaactgccg tcaacgtgca caaagggatc gcgacagcga
     5401 accgcatcgt ccaacacgga ttggtactgg atggcgtccg tgccgtgacc atcatgagcc
     5461 cacacacagg ccgaggggac gagtgccttc gcctcccgca cgattttccc cccgcaccaa
     5521 tcgaccacag ccacatatcg tggacggccc aggcttccca cgcctgaaat ccgtcggcga
     5581 atcgcatatg gaagacccgg ttccggcatc agatgctcga gggccgcccg cgcactgggt
     5641 ggaacagccc ccttcacagg cggcaggtcc ttgatcctcc gccagaagcc agccgagtcc
     5701 cgaagttttc cggttgcgat gtcgcgaagc cattggtgtc tctctgccag cacaaacggg
     5761 cgaccttcgg ccttcagccc ttccgtatag ccgctcagga ttgcgtcaca agcatctttg
     5821 ggcttgatgg tgagaaactc ggtcatgatg gccagcttcg cgctcacggc caatcgcact
     5881 agatcgttcg tatacggcaa ggggtaggcc tcatcaaaat cgttgatccc ccagatcaaa
     5941 cgcccttcca catccagcca tgtcccgaag ttttccacat gcagatcccc gaccgccaac
     6001 acaaccggcg ccgtggttaa gtcggaacag acagtcggcc agcactgcat ccagcgataa
     6061 aacgtggctc gaaaaaaagc aaattcatcg tttctcatca atttgtgttt aaggagaaga
     6121 tcaggcgaca ccagcgaagt ctgcgtggcc aactgcatct catacgaccg agtagcctgg
     6181 acaatagtca tcacatccat agacgttagc cgaagagctg agtcggctcc atgatgagga
     6241 gaagggaaaa cggcgcaaac gccaagagcg taattcgccc gctagagatt tacttcgatt
     6301 cattcaacaa ttcgccggtg ttttccattc ctccccatta tgccgaaacc aaacaagact
     6361 cccgaatcgt tttcttgccg cgatcaggag ggaaccagtg tcacgtgcac gtctcgttcg
     6421 cgtggaccgt ccagctccac cagcattacg gattgccagg tgccgaggac cagtttgccg
     6481 gctgaaaccg gaatcgtcag ggacggaccc aggatcgacg aggccatgtg ggaccaggca
     6541 tgtgaaggat catgggcgtg ccggaagtgg atgcgtggaa tcatctgctc cacgacttgc
     6601 aggaagtcat cctcagtccc ttccccgatt tcccccgtcg tcaatgcggc ggtcgtgtga
     6661 gtgatgaaga tggagcaaag cccctcctgc atcttagccc gccggatgac agtctcgatc
     6721 cgatctgtta gatccacgac ctgtttccgg ttggtggtac tgatgcggag gacgctaacc
     6781 atctgcgact ctgcctccac caaatagagg accagttgaa atgggccccg acatatttat
     6841 catgactcag tcggataaat gaaccacagc ggccagacga gatgagtatc cttgccaaca
     6901 ccttttctct cgcgccaacg cgtcataaat aattcctggg tccatttcgc ttgcgatctt
     6961 gtttctgtcg gcataacgga cagcgagcat tcagcccctc gacgctctct tgatatccat
     7021 gtgccaggga aaatcgtaca ttgtttaagg aatgacgatc cgctacagtg atttatcccc
     7081 agaaagactt tttaaggagg tgatccgtga acctcttgct gatccatccg agcccactca
     7141 tgtatacaaa gatctatttg aggctggagc ccctcgggct cgaacttgtc gctgcggctg
     7201 cgcgtcgtct cggtcacaac gtcacccttc tcgacttaca agtagaatcg cacgagcagt
     7261 ttattcgact ccttcgtgaa cgtcgaccgg atgctatcgg gttctcttgt aattacctcg
     7321 cgaacgttcc cgaaatcatc gacctctcaa agctggccaa gcagcattta ccgcactccg
     7381 tggtcttcgt cggggggcac agcgcctcgt tcgtcgcgca cgccattctt gatcatgcgg
     7441 acggcgccat cgactgtgtg ctcaagggcg agggagaaag tacgatcgga caattgctga
     7501 atgtgatcga ggaagaccgc accgcgctga atcgcatccc cggctccgtc acgctgaacg
     7561 gtgaaggtcc gccggcccag atggtgacaa gcctcgacga ccttcttccc gcgcgtgatc
     7621 tcctgcggca tcggcgcagg tacttcatcg gcgtgctcga tccctgcgcc tccatcgaat
     7681 ttacccgagg ctgtccgtgg gactgttcct tctgcagcgc ctggacattt tacggacgca
     7741 gctatcgggt ggcgagtcct gaagcaatca tggaagatct atcccgcatt cgcgaaccag
     7801 gcgtgtttat cgtcgacgat gtcgcgttca ttcagggcaa acacgcaatg gccatcggtg
     7861 aagccatcgc tcgacacggc atcaagaaac aatactactt ggaaacgaga ggcgacgtgt
     7921 tgctccgcaa taaagaagtc tttcaattct ggaagacact gggactcacc tatatgttca
     7981 tcggcatcga ggcgattgac gccgaagggc tcgcgctcca tcgtaaacgt atgaccatca
     8041 gcaaaaactt cgaggcgctc gagtatgccc gctcgctcgg tatcacggtc gcgatcaatc
     8101 tgatagccga tccgagctgg gaccgtcgcc gcttcgaaat cgtccgccaa tggtgcctgg
     8161 agattcctga aatcgtgaac atcagcgtga atacgcctta ccccggcaca gaaacctggc
     8221 atacggaaac gcggccgatc acgagccgcg actacaggct ctatgacatc cagcatgccg
     8281 tcctccctac gaccctcccg ttgccggact tttatgagga gttggtgaag acccagcaga
     8341 ttttgaatag gaagcacctc ggatggaccg cgttgaagtc caccgcgcgg atcgccgcag
     8401 atcacttgct gcatggtcag acgaatttcg tgaaaatgct ctggaagttc aacagcgtgt
     8461 ataacccgca actccagctt gccgatcacg ccaaaccggt cacctatcag atgcctctcc
     8521 cgcagaaacg acaccaagga ctcgacaacc atctgctcta tatccatcga tctcaaggcc
     8581 gggagggacg tctcttggat gacgccaccg aacggttcgt cgaagcgact cgtagcagtg
     8641 cggcaaacta gatcggatga atcccgcgag cctgaggtga cggacgtggt tgtcaaaaca
     8701 catgatcgtg tttcatctgg cgcgcggacg aggcgaacga cggttccaga ccctgcgcct
     8761 gattattcaa tgcccagaag cttagcggcc tttttgacgt ccttcgtctt gacccacaga
     8821 atggcgccga agcgtccctg accggcaacg acagcggtca cggcagtcaa attgattttg
     8881 gccaatccaa gttggcttgt cacctcgacc agcgctccgg gacgatcatc accctccacc
     8941 aagaagcata ccttcggtcc ctgcactttc cacttcagcg acttcgtcgc atccttaaaa
     9001 ccagtggcat cctccggcac cacatccact tgtgtacgac gattccgagg aaacgcatgc
     9061 accgccaaca ggctcactcc ggcatcctgg agtggagcca gcatgtgagc taacgtccct
     9121 gacttgtccg cgatggtcac cttgaaatat tgggcagctc gaatcagatc ggccatcagg
     9181 tcctcctcta cccgcccgca tagcgggtat cctgcagcaa atcatgctcg gcgatgaaag
     9241 aaggtgcgtg gggaatcatc atcacggcga cgtacctccg cagcaggctc gtcatgatgg
     9301 gatggagtca atcagatata gcctagtcgc ctactttgag caatgcaacc gggccgattc
     9361 cttatttccg cgagccggtt aactatgtat aagcccaatt gaacaagcaa cgccgcgcgt
     9421 ggatgagtca ctccgccaaa gactccaatc cattttattc tcgttaagaa tgaactggtt
     9481 atgaacggga ctaccataca caggcaggaa cgagagggat gtgcgcccac agaaatttga
     9541 aacgaatctt gcggatcttt atccttacaa tatctcgcat tccggagcgt tgacttacaa
     9601 cctccacggg aataggctca agcgtgtttc gtatcccgga tgttgccagt ccaatcgttc
     9661 caccaagagg gggttggtat ggaagacctc agtcccccgt gccagactgg cccgccgatg
     9721 tgacggcggg cttctgatcg gtcgcccgta gcaacaatct caaaggccat gcctctcggc
     9781 agagagagta acgcgtttgg cctgcaggaa gggccttccg gattcaggat gtcagacacc
     9841 ggccgagggc cgtaccgatc accccgcgat cagaagtccg accagccgcc cacaagacgg
     9901 gtctcctctc accatcgtcc attgcctggc gtcttaagcg tctgattccg gctttttctc
     9961 tgaaaaagaa ggcgcccgaa tcagggaaat atctagctca attcgccacc cgatgttctc
    10021 ttcttctatg gtgaaaccca tggctacgat aaccaaaagc gacaagtttc tccccggaga
    10081 agaacaaacc tccgcgacca gaacgccccc gaagaaaatc ctcgtgaccg tggatgattc
    10141 agatcaatct gtccgtgcca tgcattatgt gggatcgctg ctgcgagaca cacatgatgt
    10201 aggcgtgacg ctgttccatg tgctgaagcc catgcctcgt gagttgatgg agcatggcgg
    10261 ctccgagatt ccggagatcg aggaccatct cggaacccag ttacgcaaag accaggagga
    10321 ttgggtacgg atggaggggg cgatcgaata tccaatcctg ctgaaggcgc tggagcgtct
    10381 cgggcagacc gggttcccta ttgagcaagt cacgctcaaa ttcgggcatg aacgcgatat
    10441 tgccgacacc attatcgatg aagccagatc gggaggctac ggcaccattg tcgtcactcg
    10501 tcaagagact tcgagcgtca agcgactctt tggcaatggc atcatcgatc gcttgttacg
    10561 ggacgtttcc ggagtcgcaa tctgggtgtt gagctgaggc gagcgacgtg cagggaacgt
    10621 cgtgccattg ttgcgggatc gcgccggggt tcgccatgaa acgaatcaat attcccgtag
    10681 gatggtcaaa aagcccgttc agcaaggcca cagcaagtga ggaggcaaag cgtactctgt
    10741 gccgtacgtt gagcctccaa acgtcgcgag aacgacgctg acgggttttt tcaccatctt
    10801 gctaacggag gtcgccatgg actgtacacg ttgtgaggcc actctcccgc tgaaagcccg
    10861 cttttgtcct tcctgcggta gcaaggtcac gctatgggaa gagctgatgc tcgatgagcc
    10921 tgtttcacgg agccctgaaa gtgcagagga gcacggggaa cggacctctc cactaccccg
    10981 ggacaatgag gagctgaggg ccaggctcat cgagtgttat ctcaactttt ccatatccaa
    11041 ggaactctcc gaatggttgc aggatctcgg actcccttca accggcacga cgcaagagaa
    11101 gctcgccggc ctgcggcgac atgccggttc gctcgtgctt ccagctgaga gctttcctcg
    11161 gcagaccatc tattacttga acaagtatga cgaggaggtc ctttcggaga tttgccagga
    11221 acttggaatc gacagcaccg ggccgaaaga aaccctcctc acccggatct accgcgaagt
    11281 gggtctccgc gagggctgga ttcagccgct gtcggaagat gcccggcgga tcatcacaga
    11341 gaccttcgtc cccatcctga agagttttga tctcaagaag gactactatc tcgacctgtg
    11401 gggtgaatta tccgatgtgc tcggcgaaga caatgtgcgc ctgcatgttc cccaagccta
    11461 cgggagcgcc attattgcgg tgttgatccc cgggtatttt caagaggctc agatgaccat
    11521 gcttcagaat gaactcaagg atagagccga aaaacgctcc tcacagcgcg cggcacctgt
    11581 cgtcgagtct gtgacaggac agggctgagc acctttccgg ccacgaagcg gcattggtcc
    11641 tgttcatgag gtgatatatt gttggaggcg ggcgatcagg tcctcttttt cctctatcat
    11701 taacgtaact atatcccgga gcgagacgat cccgatgaat tcatcgctat cgaagaccaa
    11761 cagatgacgg cagcgatgtt ccttcatcag attcaagcaa tagttcgccg tatccttcgg
    11821 gctcacaatg accacctttt tggtcatcac atcattaatc ttgaccgtat caggatctcg
    11881 cttcttgccg accacacgca tcatcaagtc ccgctcggtg aaaatgccgg tgattttaga
    11941 tgaactggtg atgaccgcag atcccacaaa ctcctcggcc attagtgttg cggcctcgag
    12001 tacggagtga cttccgtcga tcgtcacaac cctgcggacg atttttgagg caatactcca
    12061 ggccatagcc gcctcctttc tgtcagatgc atggggatac catctgcacc tatgccaagt
    12121 tgacggggca cggggaaacc ctccccctcc gtcgcagcat ggttgattcg ccatcataac
    12181 ccacgccatc taaattctag caggctttgc cgacactgtc cgtgacctcc tccattgctg
    12241 tattcccaca gttcacaaat agttttgaac gccctgctgg gggtgagcat cgatccaaga
    12301 tgccaacgcc cccgtctcac cggatgacca ggacagaaca ggccactttg tgcaagacgg
    12361 catgggagac gctgccgagc aggaagtgct ccaggccttt ccagccatgc gacccaatca
    12421 ccacaagatc ggcaccgcgc gccgccagcg taatgcaatg ggccggatca ccatgcat
//
LOCUS       DATIFR010000142         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_285854, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000142 DATIFR010000000
VERSION     DATIFR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285854"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VLD60_08675"
     CDS             <1..528
                     /locus_tag="VLD60_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45072.1"
                     /translation="LSMGFGDSRAWWIIDMARLEADRAAGRVRDLSQEVPRGLPQARE
                     ALETFLTALPRSLPIDYTQAVIGGFSQGAMLTCDHVMRTAHPFAGLIQLSSSLLAKQD
                     WRPTVAKRTGLPVFQSHGTQDPILPFVMAERLRDELARAGLSVEWHSFRGGHEIPETV
                     LRHLGAFLTNVLNKP"
     gene            525..1325
                     /locus_tag="VLD60_08680"
     CDS             525..1325
                     /locus_tag="VLD60_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB45073.1"
                     /translation="MSMLEPFFLVGPDGKRIRGDRAAGSDRQILFITGFLSKRWGNKS
                     KALAQWCQEKGWGFCCYDVRGFGDSEGTFTDYTLSNWIGDARTVISMLKSEQNLTLPP
                     QARQDAPLPRITIVGNSLGGWIAWLMAQEFEQIERLILIAPAFNMMGVRAKSIDPERQ
                     QAWRRARWMPWDDDPVHKEWPLSWKWVEESEAYWKTSFDRLRRVKTTILHGLQDTVIL
                     PNGSSEFVEQLLLHDPAYPVELDLIPGDHRLSSPEHVERFHRLVLDNV"
     gene            1335..1502
                     /locus_tag="VLD60_08685"
     CDS             1335..1502
                     /locus_tag="VLD60_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45074.1"
                     /translation="MLTLIHKECGGPAMEDSPIGEVCAIPIDRFPFPCFTCLEEILDE
                     SELRLSEELGI"
     gene            1584..>3011
                     /gene="ettA"
                     /locus_tag="VLD60_08690"
     CDS             1584..>3011
                     /gene="ettA"
                     /locus_tag="VLD60_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HSB45075.1"
                     /translation="MATTNDKQVIFSLVNVGKVYPPKKQVLREIYLGFYYGAKIGVLG
                     LNGSGKSSLLRIIAGVDQNYTGEITRSKGYSVGLLEQEPQLDPNKTVKEVVEEGKKEL
                     VALLKEYEDVSNKMGEATPDELEKLIDKQAQLQEKIEAANGWELENELEVAMDALRCP
                     PADQKVGTLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTV
                     IAVTHDRYFLDNVAGWILELDRGHGIPFQGNYTSWLEQKKDRLEKEEKAESKRRKTLE
                     HELEWIRMSPKARQSKGKARLNRYEELVNQRQEQVAEDLEIYIPPGPRLGDVVVEAKG
                     ISKAFGDKVLYENVNFNLPKGGIVGVIGPNGAGKTTMFKMIIGKEKPDAGSIRIGETV
                     KLGYVDQDRSLDPNKSVYDVISEGHDTIKLGKADVNARGYCARFNFAGTDQQKKVKDL
                     SGGERNRVHLARMLKEGANLIILDEP"
BASE COUNT          699 a          840 c          897 g          575 t
ORIGIN      
        1 ttgagcatgg gtttcgggga ctcacgggcc tggtggatca tcgacatggc ccggctcgaa
       61 gcggatcgcg cggcaggacg tgtgcgcgat ctgtctcagg aagtgcccag ggggctgccg
      121 caagcgcgcg aagctctgga gacttttctg accgccctcc cgcgctcgtt gccgatcgac
      181 tatacgcaag ccgtcatcgg cggattttcc caaggcgcta tgttaacctg cgatcacgtc
      241 atgcgcacgg cgcatccgtt cgccgggctc atccaacttt cgagctcgct cttggccaag
      301 caagattggc gacccacagt cgcgaagcga acgggactgc cggtcttcca gagccacggt
      361 acacaggacc cgattctccc ctttgtcatg gccgaacgat tgcgtgacga actcgcgcga
      421 gcggggctct cagtcgagtg gcacagtttt cgtggcgggc acgaaattcc ggaaaccgtc
      481 cttcggcact tgggggcctt tctcacgaac gtgctgaaca aaccatgagc atgctcgaac
      541 cgttttttct tgtcgggccg gacggtaagc ggattcgcgg ggaccgcgcc gccggtagcg
      601 atcgccagat tctctttatc accgggtttc tctccaaacg ttggggcaac aagagcaagg
      661 cgctggcaca atggtgtcag gagaaaggct ggggcttctg ttgttacgac gtgcgtggat
      721 tcggcgactc agaaggaaca ttcacggatt acacgctctc gaattggatc ggtgacgcga
      781 ggactgtcat ctcgatgctg aagagcgagc agaatctcac cctcccaccc caggcgcgcc
      841 aagacgcgcc tcttccccga atcaccattg tcggcaattc cctcggaggc tggattgcct
      901 ggttaatggc ccaagagttc gagcagatag agcgactcat cctcatcgcg ccggccttta
      961 acatgatggg cgtgcgcgcg aagtcgatcg atcccgaacg ccagcaggcc tggcgccgcg
     1021 ccagatggat gccgtgggac gacgatcccg ttcacaaaga atggccgttg tcctggaaat
     1081 gggttgagga aagcgaagcc tattggaaaa cgagcttcga ccggctgcgt cgcgtgaaga
     1141 cgacgattct ccatggcctt caagatacgg tgattcttcc gaacggaagc agcgagtttg
     1201 tcgaacaact gcttttgcac gatcccgcct atccggtcga gctcgacctg atccccggtg
     1261 atcaccgtct cagcagcccc gagcacgtcg aacggtttca tcggctcgtc cttgacaatg
     1321 tatgaggtgc cacgatgctg acattgattc ataaggaatg cggcgggcct gcaatggaag
     1381 actccccgat cggcgaagtc tgcgccatac cgattgaccg ctttcctttc ccctgcttca
     1441 cgtgcctcga ggaaatcctc gacgagtcgg aactgaggct ctccgaggag ctcggaatct
     1501 agccacttcg tgttgtgaaa gcccgccggc ttttgttatc gtgagccttc cggtttcgaa
     1561 tcatccatca caagaaggaa gacatggcca ccacgaatga taagcaggtt attttttcac
     1621 tcgtcaatgt cggcaaagtg tacccgccga agaagcaggt gctgcgggaa atctatctcg
     1681 gcttctatta tggcgccaag atcggcgtgt tgggattgaa tggatcgggc aagagcagcc
     1741 tgctcagaat catcgccggc gtcgatcaaa actacacggg cgagatcact agatcaaagg
     1801 gctattcggt cggcttgctt gaacaggagc cgcaactcga tccgaacaaa acggtgaagg
     1861 aagttgtcga ggaggggaag aaagaactcg tcgcgttgct aaaggaatac gaggacgtca
     1921 gcaacaagat gggcgaggcg actcccgacg aactggaaaa gctcatcgac aagcaggcgc
     1981 aactgcaaga aaagatcgaa gcggccaacg gctgggaact ggagaatgaa ctcgaagtcg
     2041 cgatggatgc actgcgttgt ccgccggcag atcagaaagt cggcacattg tccggcggtg
     2101 aaaaacgtcg cgtggctctc tgccgcctca tgatccagga gccggatatt ctgttgctcg
     2161 acgagccgac gaatcacctg gatgcggaat cggtgcaatg gctcgagcaa catctgcaac
     2221 aatacaaagg caccgtcatc gccgtcaccc acgaccgcta ctttcttgac aacgtggctg
     2281 gctggattct cgagcttgat cgcggccacg gcattccctt ccagggcaac tacacctcct
     2341 ggctggagca gaagaaggac cggctggaga aggaagagaa agccgagtcg aagcggcgca
     2401 agaccctcga acacgagctg gagtggattc gcatgtcacc caaagcgcgt cagtcgaaag
     2461 gcaaggcgcg gctgaaccgc tacgaagagc tggtcaatca aagacaggag caggtggccg
     2521 aggatctgga aatttatatt ccgcccggac cgcgattggg cgatgtcgtc gtggaagcca
     2581 agggcatcag caaagcgttt ggcgacaagg tgctgtacga gaacgtgaac ttcaacctgc
     2641 cgaagggcgg gatcgtcggc gtcatcggtc cgaacggcgc cgggaagacc acgatgttca
     2701 agatgatcat cgggaaagag aaaccggacg ccggctcaat caggatcggc gaaaccgtca
     2761 agctggggta tgtcgatcag gatcgcagtc tcgatccgaa taagtccgtg tacgacgtga
     2821 tctccgaagg gcacgacacc atcaagctcg gcaaggcgga tgtgaacgca cgaggctatt
     2881 gtgcccgctt caattttgcc ggcaccgatc aacagaagaa ggtcaaggat ctgtcaggag
     2941 gcgaacgcaa tcgcgtccac ctggcgcgca tgctcaagga aggcgcgaac ctcatcatcc
     3001 tcgacgagcc c
//
LOCUS       DATIFR010000143         1590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_93408, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000143 DATIFR010000000
VERSION     DATIFR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93408"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..>1590
                     /locus_tag="VLD60_08695"
     CDS             247..>1590
                     /locus_tag="VLD60_08695"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSB45076.1"
                     /translation="MFTQRVACLSRLVPLGAALVFLQACSGTAQTAANLDQSYQYEDT
                     RELMALVTDATELVRTKGEEALSEFRVSGSRWRQGDTYIFVLDPKGNMLVHPDAAMEG
                     KNELDLKDINGKPIIRGLLGAAMTFPGKPEGWYHYEWPVPGGLLPRWKSSYVRLVTAP
                     SGNRYVVGSGMYNDRMEPPFVVDAVKDAVGQIEQHGTAAFPLFHDPTGPFIAKDAYIF
                     VFDHKGIDLVNPAFPNLEGRNLLDLKDAHGKPLIREMFKVVQTSGSGWVNYMWPKPGE
                     SVSTQKFAYVSRAKMGNQWVLVGSGVYLGDAPKAAPVANKMTAPELMTLVREAAAVFE
                     QRGEAAYDEFRKPGSKWFRDDIYFFVWTMHATRVFHAADPGLEGRNERDLKDIQGRPF
                     GRMFLDAGGSPSGEGWVHYMYPEPGNIFPMWKSSFIKRVKFPSGKEHIIGCGIYNM"
BASE COUNT          336 a          435 c          472 g          347 t
ORIGIN      
        1 aggctgttgt gtcgcctctt gcaccagcct tcgtcgtctg catgttgaca aagtcctcac
       61 ttctctcgtg atagtcagcc gcctcccctc ttgtgaacca cgctaactgc ttcactttca
      121 tggattctgc ttagaccgtc aatcgcggca cgtctgttgc tggttcctcc cacctatctg
      181 acttggggcg ccgctgttga agaccccttg tcgagatatc ggagcgacat tacggagggt
      241 ctgcacatgt tcacacaaag agtggcttgc ctctcacgat tagtcccgct cggtgcggca
      301 ctggtctttc ttcaagcgtg cagtggcact gcgcagaccg ccgccaactt ggatcagtcc
      361 taccaatatg aggacactcg cgaattgatg gcgttggtga cagacgcgac agagttggtc
      421 cgtaccaaag gagaggaagc gttgagcgag ttccgtgtct ccggcagccg ctggcgccag
      481 ggagacacct atatctttgt attggatccc aaggggaaca tgttggtgca tcccgatgcc
      541 gcgatggaag gtaagaatga attggacctg aaggatatca acggcaaacc catcatccgg
      601 ggactcctcg gtgcagccat gacgtttccc ggcaaaccgg aaggttggta ccactacgaa
      661 tggcctgtcc cgggcgggct gctgccccga tggaaaagca gctatgtccg gctcgtgacc
      721 gcgccgtccg gcaaccgcta cgtggtcggg agcggcatgt ataacgatcg catggaaccg
      781 ccgtttgtcg tggatgcggt gaaagatgcc gtcggacaga tcgagcagca tggcacggca
      841 gcgtttcccc tcttccacga tccgacgggt cccttcatcg ccaaggatgc gtatattttc
      901 gtgttcgatc ataagggcat cgatctcgtc aatccggcct ttcccaatct ggaaggtcgc
      961 aatctgctag acctgaagga cgcccacggc aagccgttga tccgtgaaat gttcaaagtg
     1021 gtccaaacca gcgggtccgg ttgggtcaac tacatgtggc cgaagccggg agaaagtgtc
     1081 tccacgcaga agtttgcgta tgtgagcagg gcgaagatgg gaaaccaatg ggtcctggtg
     1141 gggagcgggg tctacctggg ggatgctccc aaagccgctc cggtcgccaa caagatgacc
     1201 gctccggaat tgatgacgct ggtccgggag gcagcagctg tcttcgaaca gcgcggcgag
     1261 gcggcatacg acgaatttcg caagccggga tccaagtggt ttcgtgacga tatttatttc
     1321 tttgtctgga caatgcacgc gaccagagtc ttccatgccg cggatccagg cctggagggg
     1381 cggaatgagc gtgacctcaa ggacattcaa ggacggccct ttggcaggat gttcctcgat
     1441 gcaggtggaa gcccctcagg cgagggctgg gtccactaca tgtatcctga gcccggaaat
     1501 attttcccga tgtggaagtc cagtttcatc aagcgggtaa agttcccttc ggggaaagag
     1561 cacatcatcg gctgcgggat ctacaacatg
//
LOCUS       DATIFR010000144        24478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1625961, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000144 DATIFR010000000
VERSION     DATIFR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625961"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..945
                     /locus_tag="VLD60_08700"
     CDS             289..945
                     /locus_tag="VLD60_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HSB45077.1"
                     /translation="MLTSLLIALRTAVVFFYLPALVYSGSVSTSEDAGWELVADDDGI
                     KVYARSLANSPVREVRAITEISATPNRVFAVLLDSDHFVEFMPYIVEARTVARDSSSI
                     WYLYQRISPPLVSDRDYTLRHQSVEDPQHGRYELRWEAANERGPTARDGVVRVEICTG
                     TYVVEGIDESARSRLTYQLHTDPGGMLPKWLADRANIESVPALLRAVRRRTMDPNYHR
                     "
     gene            complement(1081..1230)
                     /locus_tag="VLD60_08705"
     CDS             complement(1081..1230)
                     /locus_tag="VLD60_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45078.1"
                     /translation="MKRVLRRNPAPVLIFIILIVAASGTLAADDQFQSTVDGLPNHQT
                     CSQAV"
     gene            1435..2331
                     /locus_tag="VLD60_08710"
     CDS             1435..2331
                     /locus_tag="VLD60_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSB45079.1"
                     /translation="MRDGQTTNYYMLLELLPTATDADIKKAWHEQLQVWHPDRFNHSP
                     ALHQKAEARTKLINQAYQTLSDPALRARYDATAQPLAAHRSPPRPSPAPASSSEAYAS
                     PPPRSQQTPRSRQEPRGPQSLIMLSRTGQPKTLVPAITMLVDSHEQLPYTFEGLTRIA
                     GTIRQTLPAGDYAIAEAPEIFCVERRRVEEFNTIFSNPTDNRMPFLQEIEPLLDYPHR
                     FLVIEGTLQYSKGGGRLGQYHKNGMMDFLDSLTARFGITIIFSDSREEAEERVANLAA
                     MHYAYHYAEQQGFGRCLTENDL"
     gene            complement(2340..2822)
                     /gene="trmL"
                     /locus_tag="VLD60_08715"
     CDS             complement(2340..2822)
                     /gene="trmL"
                     /locus_tag="VLD60_08715"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HSB45080.1"
                     /translation="MVHFAPMFDVILYQPEIPPNTGNIIRLCANTGARLHLVKPLGFT
                     LEDKQLVRAGLDYHEFATVTVHEQWTACAKHFEGKRVFAVSTKGAQRYDVNRYVPGDV
                     FLFGPETRGLPAELLNGFSEARRIRLPMLPESRSLNLSNAVAVVIYEAMRQSNFGPCQ
                     "
     gene            complement(2992..4011)
                     /gene="galE"
                     /locus_tag="VLD60_08720"
     CDS             complement(2992..4011)
                     /gene="galE"
                     /locus_tag="VLD60_08720"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSB45081.1"
                     /translation="MILVTGGAGYIGSHICVELLQAGFNVTVFDNFSNSHPEALARVQ
                     RITGKPLRIIRGDCRERVALVSALRESEADSVIHFAGLKAVGESVQQPLAYYDNNVVG
                     SLRLLEAMNECGVRKLVFSSSATVYGDPQRLPLTEEHPLSATNPYGRTKLVVEEMLRD
                     LHRSDVCWRICILRYFNPVGAHASGLIGEDPRGVPNNLLPFVGQVAVGRQERLNVWGN
                     DYPTQDGTGVRDYIHVVDLALGHLKALEALDRLDRQADCLTINLGTGSGHSVLEIVRA
                     FEKASGKPVPYSFAPRRQGDVASCYADPNRAFTLLGWRAERGLSEMCTDAWRWQSRNP
                     NGYQS"
     gene            5172..6815
                     /locus_tag="VLD60_08725"
     CDS             5172..6815
                     /locus_tag="VLD60_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HSB45082.1"
                     /translation="MSASSALLAPTVSIRTFRPTNVRRILCVFPAYTKAFGTFDHAFP
                     LMGPVKAFMPPQGILLIAALVPQEWEVRFVDENIRPVAESEFAWADAVLVSGMHIQRR
                     QILDLGRRAHRAGKVIALGGPSVSAAPEDYTEIDLLHCGEVGDGTLDLFRRLDKTAAR
                     PERQIVYRTVDRLPMTQFPTPAYRLIDVRQYLLGCVQYSSGCPFTCEFCDIPALYGRN
                     PRLKSPQQILAELDELADGGTPSIYFVDDNFIANPKAALDLLPHLVEWQRRRDYTIRL
                     SCELTLNVAEHPEILKLMREAFMTNVFCGIETVEPDALRAMSKRQNLRTPILQSVDTI
                     NSYGIEVAAGIILGLDTDTPETPDAILEFIEAAQIPIVTPNLLVALPHTPLYKRLQKA
                     TRLNSGEGRDSNIEYLQPYETVVANWKRVIRETYEPRKIYARYATQAKKTYPHRKRPV
                     RPLDQLTWANVRRAIEVFSRTAWRVGICSDYRREFWMMTWRELREGNVESVFQIAMVA
                     HHLITFGRECLTRDVQASAYSARGREEYAPSSPVTESCL"
     gene            7337..9094
                     /locus_tag="VLD60_08730"
     CDS             7337..9094
                     /locus_tag="VLD60_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45083.1"
                     /translation="MKKLLPFHMQDTAVQPRAHAIALLLAGMFTIGPVAFAEDNPPVL
                     SEQKIAERARAAWENGAANGALDLLDQGIQDHPQALTLYKLRGDILATSRGAQEAVRA
                     YDTVLANQPTALDVHWAKWSVLSRSGQGEESVDELKRMAQVDARNPLIHFRLAQELRK
                     LDRLEESLESYQRAVELVPDLLGWRLALARARFDVLDYQTAETDVLYVLQHIPPGSPL
                     ELPANNLLSQIHGTSIDRGRRFDPVLTRDMTPGQRKEWAAIRAEAWKLFSTGHYREAE
                     PIYRRLLVLNPKDPIATHQLGLTLMQLGRCKDALEVFGKMSDLDPSDEDYADTTFRMG
                     QCLVELERWEDAFVHFQILYDAAVEFEAANKNVELPPDTRVLDKKKLARWLDKVRPHV
                     PELAKLQAETAAEANGPTADAPDSLLLSEEELYKKALEQLQPQKALDTRTSLMGRDAD
                     FSWFRFVIPAAKVMRDDFPTGAHEFIPLNPGDTFLTTQPEIYLVFGLVSSSYDAVPLT
                     ALCVVETAESAGEQRPVAQDRAMTSMNDQSGYFMLTPPQTGWTPGLYRCGLFAGERTS
                     ADTQVDEVRFRIVQPTTPS"
     gene            9121..9903
                     /locus_tag="VLD60_08735"
     CDS             9121..9903
                     /locus_tag="VLD60_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSB45084.1"
                     /translation="MIRPTFRIAFLHLAPAPGDLAANRRAIEAGVIRAARQGAKWILT
                     PELAVCGYTFADTIGTNWIQPQPDPWVAGLCRQIARLRVTLFLSHPERDHNTGRLYNS
                     LLVITPDGQLIASHRKINTLKVGSESWSSAGDQARPIVVAPVGPVGLLICADAFSPSI
                     AQQLKAQGARVLVSSAAWAPGLHGPNGEWERCTKDTGLALLVCNRTGPDRTLNFTKAE
                     SVVAKEGRKLLSLQADQAALFLIEWDLATQTLASTRFRQINL"
     gene            complement(9991..10539)
                     /locus_tag="VLD60_08740"
     CDS             complement(9991..10539)
                     /locus_tag="VLD60_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB45085.1"
                     /translation="MMKTSIRSIGSTGLILAVLGLVASPSVGLALTQILPEAVIEADQ
                     QSLAELIGTFDRAQEAIRARDIDALMGLYAQNYQYHGLKRGDIRKIWADLFARYDLIA
                     NMHTFSAIKVVTVGKQTTAEITCTGAVWANSKETKQRIPIDSWQREVHRLVKGKDGWR
                     IVGNLGGDQTIKQFGTAPHPLF"
     gene            complement(10983..11204)
                     /locus_tag="VLD60_08745"
     CDS             complement(10983..11204)
                     /locus_tag="VLD60_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45086.1"
                     /translation="MKGAVVEYCSAVDLMPQDSLLRNEFAQALEANGAQAEAVGERRR
                     FLELHPNQADQRALRQGAGAKLRALGAKP"
     gene            complement(11216..11767)
                     /locus_tag="VLD60_08750"
     CDS             complement(11216..11767)
                     /locus_tag="VLD60_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45087.1"
                     /translation="MPLSPTPVTASEAKQAETLLRAIEESRTALAGNPDNAELRLRLA
                     LALESHGDIDDAIKGYRAVIVRDPSYVYAYNNLGVLYARKGLYEEAIGKFGDALFRDS
                     QHESARFNSGLIFRKTGELDPAARQFQLLLKANPKHAEPHARLGQILDDQGLSNRPSD
                     ITGKPLPSSRSSAPATVLSPKPL"
     gene            complement(11935..12621)
                     /locus_tag="VLD60_08755"
     CDS             complement(11935..12621)
                     /locus_tag="VLD60_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45088.1"
                     /translation="MLYSALCTQISALLFLFGCAIPQVPFRTIYEDPVNYVRLEVDDT
                     VLPEWPPGHHSHPRVFTVDQVTRVLKGMTVQDHRIWLQKWIQGEAPFVPAFRDEEVAL
                     LAPYITEAFAAARHDERVTFYLSQPQTSVKRVFTSGGMYFRGNELHLILGNWQIVYGI
                     PTYGMIYDRRYPMRPTAAKGFDLFFQPAEAIVKQRSSLWDTILANDTDELVIDTGLIG
                     PSGVASALVP"
     gene            complement(12691..13200)
                     /locus_tag="VLD60_08760"
     CDS             complement(12691..13200)
                     /locus_tag="VLD60_08760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB45089.1"
                     /translation="MSEPTGETRATISVSSKGQAIGDIVLKFFHDVAPGHVKNFTKLA
                     KEGFYNGTTFHRVIPGFMIQGGDPNSKSADRASHGMGGPGYRVKAEFNSKPHKRGVLS
                     MARANDPDSAGSQFFICVADANFLDWQYTAFGEVVSGMDVADKVVNMKRDGRDNPLER
                     VEVTVTVTD"
     gene            complement(13221..14249)
                     /locus_tag="VLD60_08765"
     CDS             complement(13221..14249)
                     /locus_tag="VLD60_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HSB45090.1"
                     /translation="MSFRLLTLALLLSISTSADSLWAQQSGTTPGPFDQQAASIQALT
                     IVGQDVVYAGSFGLGMFRSDDRGATWTKSGEGVTDPFILSLVTGKDGSVYVGTFRGGV
                     FRSKDQGKSWQVLNGGLKRKEIKTLLAVGDVIYAGTADGAYRLNTADDRWSVVTTGLD
                     EVLVHALAQGADGTLYAGTSGKGVLRFKANTTGWVRMQHGLKDHEGMIENFIRVLTID
                     PEGGIYAGTFDGGVFRSTDGGQTWRPISRALPNDSIRGIVFNSRGLFVATGHGIFKTT
                     DKGRQWVPLNNGLTSMAVQVLIESGTGSLYAGTNAGAFRSDDDGKSWTAINQGLEGGV
                     APPPFLFR"
     gene            complement(14260..15033)
                     /gene="tatC"
                     /locus_tag="VLD60_08770"
     CDS             complement(14260..15033)
                     /gene="tatC"
                     /locus_tag="VLD60_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSB45091.1"
                     /translation="MPPIQNPLAAHIQTIKKRLIIIGATILVSLMVAFTFSAEMVAWL
                     NRPFPNALVFYGPTEALFASIKVSFLAAVLASLPVVFYQFWKFVEPALLPKEQRWGIP
                     IFLLAGGLFALGLVFCNLVILPLVIDFFVSFGLDRDITPQLSVGTYIDFNVKFLLVFG
                     CAFELPLVLTILARVGVVSAQVLAKYRKHAILSALIISAIVTPDATLFTMMLMAVPLM
                     VLYEIGILGARLFGRNTPTNVTLPLDPDLPMGTAGHRVR"
     gene            complement(15082..15324)
                     /locus_tag="VLD60_08775"
     CDS             complement(15082..15324)
                     /locus_tag="VLD60_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HSB45092.1"
                     /translation="MLTDNVIAERLRQSSTEFRELEESHHRLDMELNELQKRHVLTPA
                     EELTKKQLQKEKLAKKDKMAELIRVHRDHGLQPAAH"
     gene            complement(15347..15889)
                     /gene="rimI"
                     /locus_tag="VLD60_08780"
     CDS             complement(15347..15889)
                     /gene="rimI"
                     /locus_tag="VLD60_08780"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSB45093.1"
                     /translation="MTYPAWIVEPATAESLSDILKIEEACFSAPWTRRMLDAELSGNP
                     FAHFVLARRQGGAEPETESIVGYLCYWIVFEEVRLMNLAVIESMRHRGIARSLVSTAL
                     QSGLSQSAHRAVLEVRASNIAAQCLYRGFGFRQVAVRSTYYNNPVEDAVLMELDPIVI
                     GSEAGHSVLHDDGGHSKPLH"
     gene            complement(15886..16674)
                     /gene="tsaB"
                     /locus_tag="VLD60_08785"
     CDS             complement(15886..16674)
                     /gene="tsaB"
                     /locus_tag="VLD60_08785"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSB45094.1"
                     /translation="MKILAVETATAWQSVAILDGDRVLARYDQDAAGSHAKLLLPAID
                     RLFKSTGLSPARLEGLAVSIGPGSFTGLRVGLATMLGFRTITQLPLAVVPTLEGMAWN
                     LRGTSLPLCPVLNSRRGELYWALFQWVGQDRIERIVLEQVGPPAALGKQLSGQVVLYG
                     EGWITEQAAIRAAMTHPVTMTETTVLEMKPSAVSIGLAGIARLQRGERAGIGVSPLYV
                     QRPEAELRYEQSGGISPVARRQEKVARKLKNRLALAGARMRRKG"
     gene            complement(16696..17529)
                     /locus_tag="VLD60_08790"
     CDS             complement(16696..17529)
                     /locus_tag="VLD60_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45095.1"
                     /translation="MWFSHRFAMRVIHHAVSVAIVCVFVTGCATTPPREEKALKQATA
                     QELTDLLRQREAAVQTMKGLFSAKVRGGIIPIATRVEGAVYYRRPNAMRMRGFTAVGS
                     ELFEFLQADDQFTLRLPTMGRVLSGSHADMSDMGKLARPFQLSVWAMGGVLGTGTVAE
                     NETATVTEDGERYRLDVSGPSRNGTQAIHRRLWFDRQTFLVVKEDRLTEAGDVDATIQ
                     YEDFRAVGEPTGESMQANGRLLRPFKISLEDGRGQGSVQVTFHELIPNAMIKPSELGQ
                     S"
     gene            complement(17513..18874)
                     /gene="radA"
                     /locus_tag="VLD60_08795"
     CDS             complement(17513..18874)
                     /gene="radA"
                     /locus_tag="VLD60_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSB45096.1"
                     /translation="MKAKTSFSCQACGHQTPRWLGRCPDCGGWNTMKEERQTATGKGR
                     PIAMKTAQAKATPIAEIEVVGEDRRLTRISEFDRVLGGGVIPGAVILIGGDPGIGKTT
                     LLLQALPRLASKEEPVLYVSGEESPRQIKMRGQRLGVEHPNLLILAETSLEQILKAVQ
                     EIQPAALVVDSIQTVYTEQITSAPGSISQVQEVAGQLMWFAKRAGVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKGHSYRILRAVKNRFGSTNEIGVFEMKDAGLEEVANP
                     SELFLAERPQRSTGSVVVSSLEGTRPILVELQALVSSTSYAMPKRMANGVELNRVSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGMHIDEPAIDLGIVAAVTSSLREVPIEPALLVLGE
                     VGLGGEVRAISQAELRIREAAKMGFKRCLLPDRNLAKVDPIDGMELIGIAEVGEALDV
                     VLA"
     gene            complement(19065..19916)
                     /locus_tag="VLD60_08800"
     CDS             complement(19065..19916)
                     /locus_tag="VLD60_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45097.1"
                     /translation="MTYLSRRALFLSFVIVILSACAAAATDKAAATLLVKDGLTSPNQ
                     PVTVDAKLVANGLLTNSALGGEPLQLLVDGKVVAMAMTGGDGKASLSYMPKAQGVMPL
                     QIRLGNSPRVAPTEGQGHLAVWERRNPILVVEMAALMQEPVPLPAVGLKIESERKPLP
                     DAAEELGKLTQFYYRVIYALPSLTGVDSFRATVEAREWLKTHKFPLGYVLVLPPGEGA
                     LGNKIDELRAAGWTTLKVGIGRTKPFAEAFLQRRLDAVIVPEPAKAETPRKAKVAKDW
                     KEVRKKL"
     gene            complement(19975..20442)
                     /gene="bcp"
                     /locus_tag="VLD60_08805"
     CDS             complement(19975..20442)
                     /gene="bcp"
                     /locus_tag="VLD60_08805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSB45098.1"
                     /translation="MAKDLAVGDKAPDISLPDQHGKTVTLKSFKGKQIVLYFYPKDDT
                     PGCTKESCDFRDVESQIVRAGGTIVGVSLDGKESHQKFIKKFGLPFPLLSDEDASISK
                     VYGVYKEKNMYGKKYWGIERSTFVIDPDGKLKAVFRKVKVDGHVDEVLTALRA"
     gene            complement(20466..20903)
                     /locus_tag="VLD60_08810"
     CDS             complement(20466..20903)
                     /locus_tag="VLD60_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45099.1"
                     /translation="MKYLCLFGAFLLLVGGAGCAKNKVKPLVPLALESGAKPQALTLT
                     EQGTQAYQGKQFEDAKNYFAQAVAAAPESGQAHYNYALALNALGDTEQARQHFIEAAN
                     LAPGDKVIWDSPALRQYGNPQSPKVVKEHPYGTTRPTMGGGPR"
     gene            complement(20930..21205)
                     /locus_tag="VLD60_08815"
     CDS             complement(20930..21205)
                     /locus_tag="VLD60_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSB45100.1"
                     /translation="MLNEEKASHLSHVILQAVKKNPLVTVKGDDGRILKDIKRVLAAE
                     LAQEEEIDRKVRAKLGSYSRGIVEGSPEWDVLYRKTFEEETRKHVKG"
     gene            complement(21303..21584)
                     /locus_tag="VLD60_08820"
     CDS             complement(21303..21584)
                     /locus_tag="VLD60_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSB45101.1"
                     /translation="MRLSKERVRHLAEALATKLVQEGHVALTGAHKAFVEALDHAVTE
                     ELSIEDRLNAEVRQMLKAHEQEIERGNVDYQKMFTMLKSKLARERGIIL"
     gene            complement(21588..21971)
                     /locus_tag="VLD60_08825"
     CDS             complement(21588..21971)
                     /locus_tag="VLD60_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HSB45102.1"
                     /translation="MMDSGRKIRITVGPVQVEAELKGTKTAQEVYAALPVEAPINLWG
                     EEFYFKLGGVKDHRETATNQVKVGDVAFWGAGQVLAIFFGRTPMSMGPDPVPADRVNV
                     IGRIVGDATVFRRVMEVPTIRVERI"
     gene            22077..22883
                     /locus_tag="VLD60_08830"
     CDS             22077..22883
                     /locus_tag="VLD60_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSB45103.1"
                     /translation="MLTSTFVLLKGIGPGTERRLWQEGIADWDTFLGHQAIPSVSCTR
                     KTWYDEILATAQAQLAAGRADYFGTCLKSRDHWRLFETFRHRALYLDIETTGLSARDG
                     QVTVIGLYRNGRMSSLVRGENLTEDRLHAELEETDLLITFFGSGFDIPFLQTAFPRLN
                     LKKPHFDLCFAARRLGLQGGLKYIERELQIDRDAALDGLDGWDAVRLWQQWRGGNESA
                     LDLLLRYNAADTENLEPLAEMLYERMVARYGPPSVGAGYPPVRIIDTGRP"
     gene            22880..23689
                     /locus_tag="VLD60_08835"
     CDS             22880..23689
                     /locus_tag="VLD60_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSB45104.1"
                     /translation="MKTSARWFGFGRTETGHVRRSNQDVFAAINSCNFWIVADGMGGH
                     PAGDLAAHIAVDVATRQAKEQLPALSEAADAAADRLGNIIIAANEAIHNKVLEEPSLK
                     GMGTTVVALTITSDPAPTAHIAHLGDSRAYRFHEGMLTQLTRDHTLVAMLLQRGLIDD
                     ATARAYPDRHILAKGLGMGLDQKPEITSMPLTNKDLLMLCSDGLTKMLDDTDIASILS
                     HAGGNPRRACHDLVEQALSRGGEDNVTVIVCAYTTTTKAGSPAIDKTLPAM"
     gene            23754..>24478
                     /locus_tag="VLD60_08840"
     CDS             23754..>24478
                     /locus_tag="VLD60_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB45105.1"
                     /translation="MLPSMRLFLCLMTATALSMSACVSMAASDNSAEAAVRALVRANV
                     EKDIHAMSKLMAHDTDITSYTIGGRKYVGWSAFEQDMLQEFSDAAKIEIPIQELTVWS
                     KGDLAWYAMELDYIRTVIQGGDTRRAVLPLRETGVLERRGGQWVLLSWHESFRNVGGA
                     ISLNDRQPQSPSLTLASTGNASASTDLGGEWEILEVEDDKRYKATLDKTGNGPYTQHG
                     GRFVTTKFSDRLWQGTWQQPGNDR"
BASE COUNT         5244 a         7488 c         6673 g         5073 t
ORIGIN      
        1 ccggtgagcc tcgcaggatc caccgggttt acctaccctc agttggcgca atggatcagt
       61 gtagcctggc tcgtcaagca tctgcgggag gattacctga agtttgtcca gcatacgggt
      121 atcaccggcc gcgactaact ggtgctcctt ctgacccgtt gggatggctc ccttgcaaac
      181 gggcagatca gcttaccaag attccgtcgt ccagccaggc gatgaacgtc aagctaatga
      241 cgctcggaac actgctctgt ccagagaaaa agtgagggag cccgtcttat gttgacttct
      301 ctgttgatag cgcttcgaac tgccgttgtc ttcttttatc tgccggcact tgtctactcg
      361 ggatccgtat cgacatcgga agacgcggga tgggaactcg ttgccgacga cgatggcatc
      421 aaagtgtatg cccgttcgct ggccaattcg ccagttaggg aagtgcgcgc cataacggag
      481 atctcggcga cgcccaaccg ggtgtttgca gtgctgctcg actcggacca tttcgtggag
      541 ttcatgccgt acatagtcga agcccggact gtcgcacggg acagctcatc aatctggtat
      601 ctctatcaac gcatttcgcc gccactggtg agcgaccgcg actacactct gcggcatcag
      661 agtgtggaag atccgcagca tggtcgttat gagctacgat gggaagctgc gaacgaacgc
      721 ggccccacag cgcgcgacgg tgtggttcga gtcgagatct gtacgggcac ttacgttgta
      781 gagggcattg atgaaagcgc acgctcccgg ctgacttacc agctccacac agaccccggt
      841 ggaatgctgc cgaaatggct tgccgaccga gccaatatcg aaagcgtacc ggcattgcta
      901 cgagcagtca gacgccgcac gatggatccg aactaccacc gttagctgcc tgtcgtctcc
      961 cagcagcctt gtcgcagcgc ttgtatctgt taaaactagc atctcattgg ggccaggggt
     1021 cttctttctt catgcgcgcc ttcatcggat tgcagtataa ctcatgctca gcatttcgag
     1081 ctaaaccgct tgagaacagg tctgatggtt tggaagccca tcgacagtcg attggaattg
     1141 atcatctgcc gcgagcgttc ccgacgcggc cacgatcaga ataatgaaga tcaacacggg
     1201 ggcgggatta cgacggagga cacgcttcat acgccactcc tttcgcccat caccggttca
     1261 aggcagggat tctgtggtgg gtcaggaggc ggtagtatct aaggaagaac cccgtatatc
     1321 tatatggggt aaattgctga ggataggacg gagcccgctg tcggttcacg gatatttcaa
     1381 tctggatctc cccccaagac ttcgttagac tttatttttc caccggagac atccatgcgc
     1441 gacggacaga cgaccaacta ttacatgctc ctcgaactct tgcccaccgc gacggacgcg
     1501 gacatcaaga aagcctggca tgagcaactt caagtatggc atccggaccg cttcaaccat
     1561 tcaccggcct tgcaccagaa agccgaagct cggaccaaac tcatcaacca ggcctatcaa
     1621 acgctcagcg atcccgcctt acgcgcacgc tacgatgcga ctgcacaacc cttagccgct
     1681 caccgatcgc ccccacggcc atcccctgca ccggcctcct cgtctgaggc ctacgcttcc
     1741 cctccgcctc gctcgcaaca gaccccgcga tcgcgtcagg agccgcgcgg gccgcaatcg
     1801 ctgatcatgc tgtcgcgcac aggccagccc aagactctgg tgccggcgat tacgatgctc
     1861 gtggacagcc atgagcagct cccctacaca tttgaaggcc tgacgcgcat cgccggcacg
     1921 atcagacaga cgttgccggc gggcgattat gccatcgccg aagcgcccga gatcttttgc
     1981 gtcgaacgac ggcgcgtgga agagttcaac acgatctttt caaatcccac cgacaaccgc
     2041 atgccctttc tccaagagat cgagccgctg ctggactacc cgcatcggtt tctcgttatc
     2101 gaaggaacgc tccagtacag caagggcggc ggacggttgg gccaatacca caaaaacggc
     2161 atgatggatt tcctggattc gctgaccgcc agattcggga tcacgattat tttctcggat
     2221 agtcgtgaag aagcggaaga acgggtagcc aacctggctg ccatgcacta cgcctaccac
     2281 tatgccgagc agcaggggtt tggacgctgc ctgacggaga acgatctgta agatcaggct
     2341 tactggcagg ggccgaaatt ggattgccgc atcgcttcgt aaatgacgac ggccaccgca
     2401 ttggaaaggt tgaggctacg gctttccgga agcatcggca gccggatgcg ccgtgcctct
     2461 gaaaagccat tcaacaattc tgccggcagc cctctcgttt ccggaccgaa cagaaagacg
     2521 tcgccgggaa cgtagcggtt cacatcgtac cgctgagccc ctttcgtcga gacggcaaag
     2581 acacgtttcc cctcgaagtg tttagcgcag gccgtccact gttcatgaac cgtgaccgtg
     2641 gcaaactcgt ggtagtcgag accggcacgg accaactgct tatcctccag cgtaaagcct
     2701 aacggcttga ccagatgcaa ccgggctcca gtgttggcac agagccggat gatattgccg
     2761 gtgttaggag gaatttccgg ctgatagaga atgacgtcga acatgggcgc gaagtgtacc
     2821 acggtcacct tgtatttcac gatcggcgaa tagtgtctat gagtgcatat caggtagggc
     2881 gagcatgtcc gcgcgacact gagggataag atcacaccga gaagtgagga cgagcactgg
     2941 gggatggaac ccgggaaatt caactacaat gatcggggac caatcacaca atcaggattg
     3001 gtatccgttt ggattgcgac tttgccagcg ccaggcatcg gtacacatct cactgagccc
     3061 gcgctccgct cgccaaccca acaatgtgaa cgcccggttc ggatctgcat agcaagacgc
     3121 cacatctccc tgtcgccgag gggcaaacga atagggaacc ggtttcccgc tcgctttttc
     3181 aaatgctcgc acgatctcca gcacgctatg accggatcct gtcccaagat tgatcgtcaa
     3241 gcaatcggcc tgcctatcaa gccgatccag cgcttccaat gctttgagat gacccaacgc
     3301 aagatcgact acatgaatgt aatcccgcac gcccgttcca tcctgggtag ggtagtcgtt
     3361 accccacaca ttcaggcgtt cctgtcgacc cactgcaacc tgaccaacaa agggaagcag
     3421 gttattcggg acacctcggg gatcttcccc gatcaaaccg ctggcatgtg cgcccactgg
     3481 attgaaatat cgcaggatac agattcgcca gcagacatcg cttctgtgaa gatctcgcaa
     3541 catctcctct acaactaact tcgtacggcc atagggattc gtggcagaaa gcggatgttc
     3601 ttccgtaagt gggagtcgct gcggatcgcc atacacagtg gcagatgaac tgaacacaag
     3661 ctttcgcacc ccgcactcat tcatcgcctc cagcagccgt agagagccga cgacgttatt
     3721 gtcgtaatag gctaacggtt gctgtaccga ctctcctacc gctttgaggc cggcgaaatg
     3781 aatcactgag tcggcctcgc tctcacgcag tgccgacacc aaagccacgc gctctcgaca
     3841 atcccctcga atgatccgga gcggttttcc tgtgatacgt tgcacgcgtg ccaatgcttc
     3901 agggtggcta ttggagaaat tgtcaaagac cgtcacattg aatcctgcct gaagcagttc
     3961 aacacagata tgcgacccta tatagccagc gcccccagtg actagaatca cgacttcact
     4021 ccgatgaaat aagactttgc ggtcacgctc ctcgcttatc cgcagcccta ctactaccgc
     4081 gcaaaagcat gattctcaac tatcggcgaa tgcgttacga cacgctgcgt acattcgtgc
     4141 ggcttccctt gcaagagtac caaaccgcaa tacaaggtca agcgatgctt cctattgcat
     4201 aacggcatga catcgaaagt ttcgcccacg gtaggtggct gggagactga gtcggcggcc
     4261 atctgatccc tatgatcagc cgcacgcctt ttcccatgct tcgaatcctt ctgctctcgc
     4321 cgcgcctctc gtgaagcaac cgagacttca atgacggagg actcgcacca tgccggttcg
     4381 tcttaactgt ctttgtctcc taccactcac agcaatgaca acggcgcgac tgcaaggcag
     4441 aggcagtgga tttcatgacg aataggcttc ctggtacagt tagcgtcgac cagcgtcgat
     4501 tcgtctccgt tttggtacat ctcccaacac caacctcagt cacactgctg tccggcaata
     4561 cattcgcgga atttccgtgc tccttggcgt gcccatggca ttgaagccgg cattagcttc
     4621 ccatgaagca atgaatgatg ccatggcaca gacgaatggg tgcggggcac ctgtgccaca
     4681 aaccgtgcgc tcgacctcct cgaccaaggg attcaagacc atcctcaaac cctcatgttg
     4741 tacaaattgc gcggcgatat gccgccacat cccggggtgt gcgggaggcg gttcgctgtg
     4801 tatcacgaaa tgatttctga gagtttttct cctatggtac aaacgtgaga gcgggtcacg
     4861 acagatcgaa ggtgagatat ggcctcggtc gcgtggactt attggagcca tcttccgcag
     4921 gacttctgca gctccggtag cttcttcgtc gctcgcatca acgtgacaat cccctcgcac
     4981 tatatgaagg aactgagcaa aagggaactg cgcaacagga tgatcggtgg tagcctgaat
     5041 taaagcgctg gataatgcca aagagtaaca catgaaggtt ccccgcatta aatgacaact
     5101 accgctaacg gtaccggagc ggattgcccg caaattgtcc ggctccgcca ttgatgaaaa
     5161 gagaggtgtg tatgtcggct tcttcggcgc ttctcgctcc gacggtaagc atccgaacat
     5221 tccgcccgac gaatgtccgg cgcatcctgt gcgtctttcc cgcctacacg aaggccttcg
     5281 ggaccttcga tcacgccttt cctctaatgg gacccgtcaa ggccttcatg ccgccacaag
     5341 ggatcctcct catcgcggct ctagtcccac aggaatggga ggttcgcttc gtcgacgaga
     5401 acatccgccc cgtggcggag agcgagttcg cctgggctga cgctgttctc gtgtcaggca
     5461 tgcatatcca gcgcaggcag attctcgacc ttggacgccg cgcgcaccga gcaggcaaag
     5521 tgatcgcgct cggcgggccc tcggtttccg cggcacccga agattatact gagatcgacc
     5581 tcctccactg cggcgaggtg ggcgacggca cactcgatct gttccgcagg ctcgacaaga
     5641 cggcggcacg acccgagcgg cagattgtat accgcacagt cgaccgcctt ccgatgaccc
     5701 agtttccgac gcccgcctat cggctgatcg acgtccgcca gtatctcctc gggtgcgtcc
     5761 agtattccag cggctgcccc ttcacctgtg agttctgcga catccccgcg ctctacggaa
     5821 gaaacccgag gctcaaaagc ccccagcaga tcctggccga actcgatgaa ctcgccgacg
     5881 gcgggacgcc gtcgatctac ttcgtggatg acaacttcat cgcgaatccc aaggccgcgt
     5941 tggacctcct gccgcacctc gtcgagtggc agcggcgtcg cgattacacg attcgcctct
     6001 cctgcgagct cacgctcaac gtcgccgagc acccggaaat tctgaagctt atgcgagagg
     6061 cgtttatgac aaatgtcttc tgcgggatcg agacggtgga gccggacgcc ctgcgggcca
     6121 tgagcaagag gcagaacctc cgcacaccga tccttcaatc cgtcgacacg attaacagtt
     6181 acgggattga ggtcgccgcc gggatcatcc tcgggttgga caccgacact ccggagacgc
     6241 cggatgcgat cctggaattc attgaggccg ctcagatccc gatcgtcacg ccaaacctgc
     6301 tcgtcgccct gccacacaca ccgctctaca agcgtctcca gaaggcgacc cgcctgaact
     6361 cgggcgaggg acgcgactcg aacatcgagt acctacagcc ctacgaaact gtggtcgcga
     6421 actggaagcg ggtgatccgc gagacatatg agccccgcaa gatctacgcg cgctacgcca
     6481 cgcaggcgaa gaagacgtac ccgcaccgca agcggccggt gcgccctctg gaccaactca
     6541 catgggccaa cgtccggcgc gcaatcgagg tgttctcgcg caccgcctgg cgggtcggga
     6601 tctgtagcga ttaccggaga gaattctgga tgatgacctg gcgggaactg cgcgagggta
     6661 acgtcgagtc agtattccag atcgcgatgg tcgcgcacca tctgatcaca ttcgggcgcg
     6721 agtgcctcac tcgtgacgtg caggcctctg cgtattccgc ccggggacgc gaggaatacg
     6781 ctccgagtag cccggtgaca gaatcctgcc tttgaagagg gcgactcgaa cagcctcaca
     6841 aatccctcat cgatctttga tcttcacaga ggaagagccc ttgtctctta tgcctgttcg
     6901 gaacctatag tccggggacc ggccgactga tggattgccg ttcgttacaa ccgattggaa
     6961 agagcgagat cctcgcgctc actttcttca ccgcacgacc tctctgaaga atatgcctcg
     7021 cgcgcggagc agatgaggta atcatcaacg tcgattgacc ctctgtactc gtccctacga
     7081 tcatgagaga cgcaggcgac accaacgacg gccggcaagg ctcaggcgtc tgttcgagcc
     7141 aaatgatggc tggcaccttt gtgactaccc gaagagaaga ctgtcgcttt tccttcttgt
     7201 cgtgaatcct cctctctcga tgtgtctctc aaagaccagg acgctgaaag aatcgcaggc
     7261 cgggacgtgg cggttcactt caccatcaca tggcttcttg gtatagtgaa ctcaatcaat
     7321 gtgggctctc ctgctcatga agaaactgtt gccatttcac atgcaagaca ctgctgtcca
     7381 gccacgtgct cacgccatcg cgctgctcct cgctggaatg ttcacgattg gacccgtcgc
     7441 attcgcggag gataatccac cggttctttc ggaacagaaa atagccgaac gtgcaagagc
     7501 tgcctgggag aatggtgcgg caaacggcgc gctcgatctc cttgaccaag ggattcaaga
     7561 ccaccctcaa gctctcacgc tgtacaaatt acgcggcgat attctcgcca catctcgagg
     7621 tgcccaggaa gcggttcgtg cgtatgacac cgtcctcgcc aatcagccga ccgcactgga
     7681 tgtccactgg gcgaaatgga gcgtcttgag tcggtccggg cagggagaag aatccgtcga
     7741 tgaattaaag cgcatggccc aggtcgatgc ccgcaacccc ttgattcact ttcggctggc
     7801 tcaagaactt cgcaaactcg accgtcttga agaatcgctc gaatcatatc aaagggccgt
     7861 cgaactggtt cccgacttgc tcggctggcg cttggcgctg gcccgagcgc gctttgatgt
     7921 cttggattat caaaccgcag agaccgacgt actttacgtg ctgcaacaca ttcctcccgg
     7981 ctcaccgctg gagcttcccg cgaacaatct cctctcccaa attcatggaa cttccatcga
     8041 tcgaggtcgg cgcttcgatc ccgtgctgac tcgcgacatg acgcccggac agcggaaaga
     8101 atgggccgcc attcgggcgg aagcctggaa actgttttcc accggccact atcgagaagc
     8161 cgaaccgatc tatcgcaggc tgctggtcct gaaccccaaa gatcccatcg ccacccatca
     8221 attggggctg acgctcatgc aactgggccg atgcaaggac gcgctggagg tctttggcaa
     8281 gatgtccgac ctcgacccca gcgacgaaga ctacgccgat acgacgtttc ggatgggcca
     8341 atgcctggtt gaattggaac gctgggaaga cgccttcgtc cacttccaaa tcctctatga
     8401 cgcagccgtc gaatttgaag ccgccaataa gaatgtggaa ctcccgcccg acacccgcgt
     8461 gctggataaa aagaaacttg cgcggtggct ggacaaggtg cgcccccacg ttcccgaatt
     8521 ggcgaaactt caagccgaga cagccgccga ggccaacggt ccaaccgctg atgctcctga
     8581 ttccctcctc ctgtcggagg aagagctgta caagaaagcc ctggagcaat tgcaacctca
     8641 gaaagccctg gacacgcgga catcgctcat gggcagagac gccgatttca gctggtttcg
     8701 attcgtcatt ccggcagcca aggtcatgag ggatgatttt ccaaccggcg cccacgaatt
     8761 tattcccttg aatccggggg acacctttct cacgacgcag ccggaaattt acctggtgtt
     8821 cggactggtg tcgtcgtcgt atgatgcggt gcccctcacg gcgctctgtg tagtggaaac
     8881 agccgaaagc gcgggagagc aacgccccgt cgcccaggat cgcgcgatga cgtccatgaa
     8941 cgatcagtcc ggctacttta tgcttacccc accgcaaacc ggttggacgc cgggcctcta
     9001 tcgctgcggt ctcttcgccg gagaacggac atccgcagat acccaggtgg atgaagtcag
     9061 gttccggatc gtgcagccaa ccacaccgtc gtaagaagcg ccggagatgc agcacacgac
     9121 atgatccgac cgacattccg catagccttt ttgcaccttg cccccgcgcc cggtgatctc
     9181 gccgcgaatc gccgcgcgat cgaggcaggg gtcatccgcg ccgcgcgaca gggcgccaaa
     9241 tggattctca ctccggaact cgccgtctgt ggctacacct tcgccgatac gatcggaacc
     9301 aactggatcc aaccgcagcc cgatccctgg gtagcaggcc tctgccggca gatcgcgcgg
     9361 ctgcgcgtga cactcttcct gtcgcacccc gagcgggatc acaacaccgg cagactgtac
     9421 aactcactgc tggtcattac gccagatgga caactcatcg cctctcaccg caagatcaac
     9481 acgctgaaag tcggatccga atcctggtcc agcgcgggcg atcaggccag gcccatcgtg
     9541 gttgcccctg tcggcccagt cggattgttg atttgcgcgg acgctttttc accctccatc
     9601 gcccagcagc tcaaggccca aggcgcgcgc gtgctcgtct catcagccgc ctgggcccca
     9661 ggcctgcacg gccctaacgg cgagtgggaa cgatgcacaa aagacacggg attggctcta
     9721 ctcgtctgta acagaaccgg cccggaccgc acgctgaact tcactaaagc cgagagcgtg
     9781 gtcgccaaag aaggacggaa actgctgtcc ttacaggcgg accaagccgc gctgttcctc
     9841 atcgagtggg atctcgcaac acagacactc gcctcgacca gatttcgaca gatcaatctg
     9901 taagacaggc gaactgaaga ttcggtggcg gccggcgtga ctgaacggtc agcatgagtg
     9961 tgtgtctgca gttcttctct tgaagtgcat tcagaacaat ggatgcggcg cggtgccaaa
    10021 ctgtttgatc gtctgatcgc cgcccagatt gccgacgatg cgccagccat cctttccctt
    10081 caccagcctg tgcacttctc gctgccagct atcgatcggg atacgttgct tcgtttcttt
    10141 cgagttggcc cataccgcac cggtacaggt gatttcagcg gtggtctgct tgccgacggt
    10201 cacaaccttg atcgcggaga atgtatgcat gttggcaatc aagtcgtatc gcgcgaacag
    10261 atccgcccag atcttgcgaa tgtctcctct cttcaaaccg tgatactgat agttttgcgc
    10321 gtacagcccc atcaaggcgt cgatgtcgcg cgcgcgaatc gcctcctgcg cacgatcaaa
    10381 ggtgccgatg agttccgcga gactttgctg gtccgcctcg atgaccgctt ccggaaggat
    10441 ttgcgtcaat gccagaccaa ctgagggaga ggctacgaga ccaagaacgg ccaggatcag
    10501 accggtgctt ccgatggatc taatggaagt cttcatcatg ccctccttcg gctaagccca
    10561 ggcgatgatt tagcgaccgg ctcccctacc tggtggatct tcttgctgaa caaaatactg
    10621 ctccccttca cgcaaacaat caagaggcgg aacggaggtg aactcgaact ggccggctag
    10681 tgagccgact ttcataaagg cacccttaga gcctgcagac ccagtatgct cgccagccag
    10741 cagatccgcg ccttcgcctt cactgaaaat ctcgtggagc cgctgcaata gggcgagcgg
    10801 gctcacgagc aggacgtgaa cgtacaacgg gacaatttga agaggttgaa cggcaacgac
    10861 tcgtgcagaa gaccagggcc gtgacgctgg cctatacgtc aggcgggagc gcgcaactac
    10921 agatcgaccc gacgattcga tagaaggcct gtcgttagca tcatgtacat gctgggcggt
    10981 ggctacggct tggcacccag ggcacggagc ttggctcccg ccccctggcg gagcgcgcgc
    11041 tgatcggcct gattcggatg gagttcgagg aagcggcgac gttctcccac tgcttcggcc
    11101 tgagctccgt ttgcttcgag cgcctgcgcg aattcgtttc gcaacaggga gtcctgtggc
    11161 atcaggtcta ctgccgaaca atattccacg acggcaccct tcacatctcc cttcttcata
    11221 agggctttgg cgagagaacg gtggccgggg ccgaactgcg acttgagggc aatggtttcc
    11281 cggtaatgtc cgatggcctg ttcgacaacc cttggtcatc caggatctgt cccagtctgg
    11341 cgtggggttc tgcgtgtttg ggattggcct tgagcaacag ttggaactgg cgggcggccg
    11401 gatcgagttc gccggtcttc cgaaagatca gaccggaatt gaagcgcgcg gattcgtgct
    11461 gcgaatctcg aaacagtgca tcgccgaact tgccgatcgc ttcctcgtag aggccctttc
    11521 tagcgtacag gacgcccagg ttgttgtaag cgtacacata cgaaggatct cgcacgatca
    11581 ccgcccgata tcccttgatt gcgtcgtcga tgtctccatg ggactcgagc gcaagcgcga
    11641 gtcgaagccg tagttccgcg ttgtcgggat tccctgccag cgcggtgcga gactcctcga
    11701 tcgcacgcaa gagggtttcg gcctgtttcg cctcagaagc cgtcaccggg gtcggcgaca
    11761 aaggcatggc cgttgacgtg ttgccggctt acacgttcca ccagccagac agagccacag
    11821 tgctccaccg aggacaacgg aacgatgccg cagcatagcc gaaacggttg agtgactgac
    11881 tgaaggcctt atgtcttctt gcatcatgac cagacgaccg agtggggagg gcggttacgg
    11941 gacaagagct gaggcaacgc cggatgggcc aatcaacccc gtgtcgatga ctaactcgtc
    12001 ggtgtcgttg gcaaggatcg tatcccacag actactgcgc tgctttacta ttgcttcagc
    12061 cggctggaaa aagagatcaa acccttttgc cgcggtggga cgcatcggat aacgccggtc
    12121 ataaatcatg ccgtacgtcg gaatgccgta gacgatctgc caattgccca gaatgaggtg
    12181 cagttcgttg ccccggaaat acatcccgcc ggaggtgaac actcgcttga ccgaggtctg
    12241 gggctggctg aggtagaacg tgactcgttc gtcatgtctc gcggctgcaa aggcttcagt
    12301 tatgtagggc gccaacagtg caacctcttc atctctgaag gccggcacga acggcgcctc
    12361 tccctgaatc catttctgaa gccagatgcg atggtcctgc accgtcatcc ccttgaggac
    12421 gcgggtaacc tggtcgacgg taaagactct cggatgggaa tggtggccgg gaggccactc
    12481 cggtagaacc gtatcatcca cttccaatcg cacgtagttg acgggatctt cgtagatcgt
    12541 tcgaaacggg acttggggaa tggcacagcc gaacagaaac aacaatgctg agatctgagt
    12601 acagagtgct gagtacaaca cgagcctccg aagccgtcgt ccgattcgtc ctattctcgg
    12661 ctcagcactc atcactcaac tctcagcact tcagtcagtt accgtgaccg tcacctctac
    12721 gcgttcgagg ggattgtcgc gtccatcgcg cttcatattg acgaccttat cggccacgtc
    12781 catcccactg accacttccc cgaaggcggt gtattgccaa tccaggaagt tcgcgtcggc
    12841 cacgcaaata aagaattgag acccggcgct gtccggatcg tttgcacgcg ccatcgataa
    12901 gacgccccgc ttgtgcggct tgctgttgaa ctccgctttg accctgtagc cgggaccgcc
    12961 catgccgtgg gacgcccggt cggcgctctt gctattgggg tctccgccct gaatcatgaa
    13021 gcccggaatc acgcgatgaa atgtggtgcc gttgtagaac ccttctttgg ccagcttggt
    13081 aaagtttttt acatggcccg gtgcgacatc atgaaagaat ttcaacacga tgtcgccgat
    13141 cgcctgtccc ttgctactca ctgaaatggt ggctctggtc tctccagtcg gttcagacat
    13201 accctcattc ctttcttgga ttatcgaaac aggaagggcg gcggcgccac gccgccctcc
    13261 agcccttggt taatcgccgt ccagctcttg ccgtcgtcgt cgctgcggaa tgccccggca
    13321 ttggtgccgg catagagcga tccggtcccg gattcaatta gcacctgcac ggccatactg
    13381 gtcaagccgt tgttcagcgg cacccactgc cgccccttgt cggtggtttt gaagatcccg
    13441 tggccagtgg ccacgaagag accccggctg ttgaacacaa ttccgcgaat ggaatcgttc
    13501 gggagggccc ggctgatggg ccgccaggtt tgcccgccat cggtgctccg aaatacgccg
    13561 ccgtcaaacg tgccggcgta gatcccgccc tccggatcga tggtcaagac ccggataaag
    13621 ttctcgatca tcccttcgtg atccttcaag ccatgctgca tacggaccca gccggtcgta
    13681 ttcgctttga atcgcaagac ccccttcccg gatgtgcccg cgtagagggt gccgtcggct
    13741 ccctgggcca gtgcatgcac caggacttcg tcaagcccgg tcgtcacgac cgaccagcga
    13801 tcgtctgcgg tattcagccg ataggcgccg tccgcggtgc cggcgtaaat gacgtcccca
    13861 acggccagca gcgttttgat ttcttttcgc ttcagccccc cgttcaacac ctgccagctc
    13921 ttcccttgat ccttgctccg gaaaacgccg ccccggaagg tgccgacata gaccgatccg
    13981 tctttgcccg tcaccagcga cagaatgaag gggtcggtaa ctccctcgcc ggatttcgtc
    14041 caggtggcgc cgcgatcgtc gctgcgaaac atgcccagcc cgaacgagcc ggcatacacc
    14101 acgtcttggc cgacgatggt caacgcttga atactcgcag cctgttgatc gaaagggcct
    14161 ggcgtcgttc cactttgctg ggcccacagg gaatccgccg acgtactaat cgacagcagc
    14221 agcgcgagag tcagtaaccg gaagctcata atgattattt taccggactc gatggcccgc
    14281 tgtccccatc ggcaaatcgg gatcgagcgg gagggtgacg ttcgtggggg tgttgcgacc
    14341 gaagagccgt gcgccgagaa tcccgatctc atagagcacc atcaacggca cggccatgag
    14401 catcatggtg aacagcgtgg catccggcgt gacaatggcg gagataatca aggcgctgag
    14461 aatggcgtgc ttgcgatatt tcgccagtac ctgcgccgac accacgccga cgcgcgccag
    14521 aatcgtcagc accagcggca attcgaaggc gcagccgaac accagcagaa acttgacgtt
    14581 gaaatcgatg tatgtgccga cgctcagctg tggggtaatg tcccggtcca agccgaagct
    14641 gacgaagaaa tcgatgacaa gcggaagaat gacgaggtta caaaagacga gtcccagcgc
    14701 aaagaggccg cccgccagta gaaagatagg aatgccccag cgctgctctt tggggagcag
    14761 cgccggctct acgaatttcc aaaattgata gaacacaacg ggcagactgg ccagcaccgc
    14821 cgcgagaaat gagactttga tcgaggcgaa caaggcttcc gtcggcccgt agaacacgag
    14881 ggcattggga aaggggcggt tcagccacgc gaccatctcc gccgaaaatg taaaggccac
    14941 cattaaggac accaggatgg tggccccgat gatgatcaga cgcttcttga tcgtctggat
    15001 atgtgcggcc aggggatttt gaatcggcgg catgggcgca gttcctgtcg ctgccggcta
    15061 tcgaacccac agcaaccctc ctcagtgtgc ggcgggctgc aacccgtggt cccggtgaac
    15121 ccgaatcagc tcggccattt tatccttttt cgccagtttt tctttctgca gctgtttctt
    15181 ggttaattcc tccgccggag tcaggacgtg gcgtttctgc aactcattca attccatgtc
    15241 gagacgatgg tgagactcct ccagttcacg gaactctgtg ctggattggc gcagccgctc
    15301 cgcgatcaca ttgtccgtca gcatgtcgca cctcccggtt gagtagttaa tgcagtggct
    15361 tgctatgacc tccgtcgtca tggagcacag aatgaccggc ttccgaccca atcacgatcg
    15421 gatccagttc catcaacacg gcgtcttcta cgggattgtt atagtacgtg gaccggactg
    15481 caacctggcg aaatccgaaa cctcgataca ggcattgcgc agcgatattg gacgcacgga
    15541 cttccagcac ggcacggtgt gcggattggg agagtcctga ttgtagggcg gtcgaaacca
    15601 gggatctggc aataccccga tgccgcatcg actcgatgac cgccaggttc atcagccgga
    15661 cttcctcaaa gacgatccaa taacagagat acccgacaat cgactctgtc tcgggctcag
    15721 ctccaccctg ccgccttgcc aacacaaaat gcgcgaaagg gttgccgctg agctccgcgt
    15781 ctaacattct gcgcgtccag ggggccgaga agcaagcttc ttctatcttg agaatatcgc
    15841 taagggattc ggcggtcgcc ggctccacga tccaggcagg ataggtcatc ccttgcgcct
    15901 catacgcgcc cccgccaaag ccaagcgatt cttcagcttt cgcgccacct tttcctgccg
    15961 gcgcgccacc ggagagatcc ctccggactg ctcgtatctc agttccgctt caggccgttg
    16021 aacatagagt ggactcacgc cgattcccgc ccgctctcct cgttgaagtc gcgcgatgcc
    16081 agccaagccg atggagactg cggagggttt catctccagc actgttgtct ccgtcatggt
    16141 cacaggatgc gtcatcgcgg cacgaatggc agcctgttcg gttatccacc cttcgccgta
    16201 cagtacgacc tggccgctga gttgtttccc taaggcggca ggcgggccga cctgctccag
    16261 aacgatgcgt tcgatgcgat cctggcccac ccattggaag agcgcccaat agagttcccc
    16321 gcggcggctg ttcagaacgg gacagagcgg cagcgatgtc cctctgagat tccaggccat
    16381 tccttccaag gtcggcacga cggccagagg caattgggtg attgttcgaa atcccagcat
    16441 cgtggctaaa ccgacacgca gtcccgtaaa ggaaccgggg ccgatcgaga cggcaagacc
    16501 ctcaagtctg gccggagaga gccctgttga tttgaacaag cgatcgatgg caggcaagag
    16561 cagcttggca tgtgagccgg cagcatcctg atcgtaacgc gccaacaccc ggtcaccgtc
    16621 aagaatcgcg acactttgcc aagccgtagc cgtttcgact gccaggattt tcatcgggcc
    16681 ctcacgcagt atccctcagc tttgaccaag ctcactgggc ttgatcatcg cgttgggaat
    16741 caattcgtgg aaagtgacct ggacgctgcc ctgaccccgc ccgtcttcca atgagatctt
    16801 aaacgggcgc aaaagtcgcc cattcgcctg catagattca cctgtcggct caccaactgc
    16861 gcgaaaatct tcatactgaa tcgtcgcgtc gacatcgccg gcctccgtca accgatcctc
    16921 cttcaccacc aggaacgtct gccgatcgaa ccagagtcgc cgatgaatgg cctgcgttcc
    16981 gttccgtgag ggaccggata cgtcgagccg atagcgctct ccgtcttctg tgactgtcgc
    17041 cgtttcgttc tccgcgaccg tgccggtccc cagcacgccg cccatggccc acacgctcaa
    17101 ctgaaagggt cttgccagct tgcccatgtc gctcatgtcg gcgtggctgc cggacaggac
    17161 acgacccatt gtgggtaatc gcagcgtgaa ctgatcgtcg gcctggagaa actcaaagag
    17221 ctcgctgcca actgcggtaa atccacgcat acgcatcgca ttcgggcggc gataatagac
    17281 cgccccttcg acacgcgtgg cgatcggaat gattccacct cgcacttttg cgctgaacag
    17341 tcccttcatg gtttgaacgg ccgcctcgcg ctggcgtagc aggtcggtca gttcttgcgc
    17401 ggttgcctgt ttgagcgcct tctcttcacg cggaggagtc gttgcgcaac ctgtcacaaa
    17461 gacacacacg atagcgacgg agaccgcgtg atgtatgacg cgcattgcga atctatgcga
    17521 gaaccacatc gagcgcctcc ccgacttcgg caatgccgat cagctccatg ccatcgatgg
    17581 gatcgacttt ggcgagattc cgatcaggca ggagacagcg cttgaaaccc atcttagccg
    17641 cttcgcgaat ccgcaactcg gcctggctga tcgcgcgaac ctcgcccccg agcccaacct
    17701 cacccaacac cagcagggct ggttcgatcg gcacttcacg caaactggag gtgacagcgg
    17761 ccacaatgcc aagatcgatc gccggctcgt cgatgtgcat gcccccaacc acattgacat
    17821 agacgtcctg gccggacaga tgcatcccca gcctcttttc catgactgcc aacaggagtg
    17881 agacacgatt caactcgaca ccgttcgcca tccgcttcgg catggcataa ctggtcgacg
    17941 agaccagcgc ctgcaactcc acaagaatcg gtcgggtccc ttctagactc gagaccacca
    18001 cggagccagt gctccgctga ggccgttccg cgaggaagag ctcggaagga ttcgccacct
    18061 cctccaggcc ggcgtccttc atctcgaaca cgccgatttc gttggtggag ccgaatcggt
    18121 ttttcacggc cctgagaatc cgataactgt ggcccttgtc gccttcgaaa tacaatactg
    18181 tatcgacgat atgctccagc aaccgtggcc cggcaatggc cccttccttc gtgacgtgac
    18241 cgatgatgaa gaccggcacg ccggctcgct tggcaaacca catgagctgt ccggcgactt
    18301 cctgcacctg gctgatgctg ccgggcgcgg acgtgatctg ttccgtatag acggtttgaa
    18361 tcgagtcgac gacgagcgcc gccggctgga tctcctgaac cgcttttaga atttgttcca
    18421 gtgaggtttc ggccaggatg agcagattcg gatgttccac acccaggcgt tgcccgcgca
    18481 tcttgatctg acggggcgac tcttcccccg acacatagag caccggctct tctttggagg
    18541 ccaatcgcgg cagcgcctgc aacagcagcg tggtcttgcc gatgccggga tcgccaccga
    18601 tcagaatgac agctccggga atcactccac cgcctaatac tcgatcgaac tcgctgattc
    18661 tggtcagccg acgatcttcg ccgaccacct caatctcggc gatcggcgtg gccttggcct
    18721 gggccgtttt catcgcgata ggacggcctt tgcccgtcgc agtctgccgc tcctctttca
    18781 tcgtattcca gccaccgcag tctgggcagc gtccgagcca tcggggcgtc tgatgcccgc
    18841 aagcttggca ggaaaaactc gtcttggctt tcatgagcgc gcagcctcaa tgaggaggat
    18901 ttgatgacct tataatagag gaaagctgag accaaaagga aggagagcac atgagggcag
    18961 cttgatcgtc ctcctgcccg cggaacgcgc accgcccttt atgcgaaaat atgacccact
    19021 ttgcgtgccg cgttgcaaaa gcaagaaggg ccactggtca ctcgctacaa cttcttacgc
    19081 acctctttcc aatctttcgc cacctttgcc ttccgcggag tttccgcttt ggccggctcc
    19141 ggcactatca cagcatcgag ccgacgctgg agaaaggcct cggcaaacgg cttggtgcgc
    19201 ccgatcccga ctttgagtgt cgtccacccg gccgcgcgaa gttcatcgat cttgttgccg
    19261 agcgccccct cacccggcgg caataccaac acatagccga gcggaaactt gtgcgtcttc
    19321 agccactccc tcgcctccac cgtcgcccga aaactgtcta ctccagtcag cgatgggagt
    19381 gcatagatca cgcggtaata aaattgtgtc agcttgccaa gttcctccgc cgcatcaggc
    19441 aaaggcttcc gttccgattc gatcttgaga cccacagctg ggagtggaac cggctcctgc
    19501 atcaacgcgg ccatctccac gacaagaatt gggttgcgcc gttcccagac ggcaagatgg
    19561 ccctgacctt cggtcggtgc cactcgtgga ctgttgccga gtcggatctg aagcggcatc
    19621 accccctgcg ccttcggcat gtacgacaag gacgccttgc catctccgcc tgtcatcgcc
    19681 atcgcgacca ccttgccatc gacaagtaac tggagcggtt ctccgccgag cgccgaattg
    19741 gtgagtagac cattagcaac cagcttcgca tctacagtta caggttgatt gggtgacgtg
    19801 agaccgtcct tgaccaagag cgtagctgca gccttatccg ttgcagcggc tgcgcatgca
    19861 ctgagaatga cgatcacgaa cgagaggaac aaggctctgc gggagaggta tgtcatatac
    19921 cgcgcgcccg attcagagag gagggcgttt gcagtggcat tgcaagacca cgaactaggc
    19981 ccgcagcgcg gtcaagacct cgtcgacatg cccgtcgact ttcactttcc gaaagaccgc
    20041 tttcaacttg ccgtccggat cgatgacgaa ggtgctgcgc tcgatgcccc aatacttttt
    20101 cccatacata ttcttttctt tgtagactcc gtagaccttg gagattgagg catcttcatc
    20161 gctgagcagc ggaaagggca acccgaactt cttgataaat ttctgatggg attcctttcc
    20221 atccaggctt actcccacaa tcgtgccgcc ggcccgaacg atctgggatt ccacatcgcg
    20281 aaaatcgcag gactctttgg tacagccggg tgtgtcgtcc tttggataga aatacagcac
    20341 gatttgcttg cccttgaagc tcttcaacgt cacggtcttg ccgtgctgat ccggcaggga
    20401 tatgtcaggc gctttgtctc ccaccgcaag gtccttggcc atgtctcgat cctctctcag
    20461 aaaatttatc gcggaccgcc acccattgtt ggcctggtgg tcccgtaggg atgctccttc
    20521 accacctttg gcgattgggg attcccgtat tgacgaagcg ccggcgagtc ccagatgact
    20581 ttgtcgccgg gagcaagatt cgccgcttcg ataaaatgct gccgcgcctg ttcggtgtcg
    20641 cccagcgcat tcaacgccaa tgcataattg taatgggctt gccccgactc aggagcggcc
    20701 gccacagcct gagcaaagta gttcttcgcg tcctcgaact gtttcccctg ataggcctgg
    20761 gtgccctgct cggtcagggt aagcgcttgc ggtttcgcac cggactccag tgcgagaggc
    20821 acgaggggtt tcaccttatt cttggcgcaa cccgctcctc cgaccaacaa caaaaaggcg
    20881 ccgaataaac acagatattt catcctcccc tcctgtacca tgggcttcat catcccttta
    20941 catgcttacg cgtctcttct tcaaacgtct tgcgatacag cacatcccac tccgggcttc
    21001 cttcgacgat gcctcgtgaa taagagccta atttcgctcg gaccttccga tcaatctctt
    21061 cctcctgcgc taattcagcg gcgagtactc gcttaatatc tttgaggatg cggccgtcat
    21121 cccccttgac cgtgaccaag ggattcttct tcaccgcttg gagaatcacg tgcgacagat
    21181 gactggcttt ctcttcgttc agcatacaga acttcgtagg agggggagtg tcaggcgcaa
    21241 ggaatgacaa ccgggtccat cttgcctgtc accttactct ttaccattca ctcctcactg
    21301 cttcacagga tgatgccgcg ttcccgagcc agcttgctct tgagcatcgt aaacatcttt
    21361 tggtagtcga cgttgccgcg ctcaatttcc tgctcgtgag ccttgagcat ttggcgaact
    21421 tccgcattga gtcgatcctc gatcgagagc tcttcggtca cggcatgatc aagggcttcc
    21481 acgaaggctt tgtgcgctcc cgtcagggcg acatgaccct cttgaaccag cttcgtggcc
    21541 aacgcctcgg ccagatgcct gacccgttcc tttgaaagcc tcatccctta gatccgttcg
    21601 acacgaatgg tcggcacctc catcacccgc cggaatacgg tggcgtcccc gacaatgcgt
    21661 ccgatgacgt tgacacgatc ggccgggacc gggtccggtc ccatgctcat cggcgtgcgg
    21721 ccgaaaaaaa ttgccagcac ttgtccggcg ccccagaatg ccacatcgcc gaccttcacc
    21781 tgattggtcg ccgtctctcg atggtctttc actccgccga gcttgaaata gaactcttcc
    21841 ccccacagat tgatcggagc ctccaccggc aacgccgcat agacctcctg agctgttttg
    21901 gtccctttca attccgcttc aacctgcacg ggcccaactg taatgcgaat tttccttccc
    21961 gaatccatca tccgactcct catgctgcct cggacatgcg gtgcatgaaa acccgcatcc
    22021 atcgcggact ctagcttgtt taatcagtga aatcaaggct acaatcccgc ctgcgtatgc
    22081 tcacatccac cttcgtactg ctcaaaggaa tcggcccggg cactgaacgc cgcctgtggc
    22141 aggaggggat cgctgattgg gatacctttc tcggccacca ggccatcccc agcgtctcct
    22201 gtacaagaaa aacttggtat gatgagatcc ttgccaccgc acaggcgcag cttgcggcag
    22261 gtcgggcgga ctacttcgga acgtgcctca agagccgcga tcattggcgt ctattcgaga
    22321 cattccgcca tcgcgccctt tacttggaca tcgagacgac gggcctgtcc gcccgtgacg
    22381 gacaggtcac ggtgatcgga ttatatcgta acggtcgaat gagcagtctc gtacggggag
    22441 aaaatctgac ggaagatcgc ctccatgcgg aattggagga gaccgatctg ctgattacgt
    22501 ttttcggcag cgggttcgac attccatttt tacagacggc tttcccacgg ttgaatttga
    22561 agaaacctca tttcgatctc tgcttcgccg cgcgccgtct gggattgcag ggaggactca
    22621 agtacatcga acgcgaattg cagattgatc gagacgccgc cctcgacggt ctcgacggat
    22681 gggatgctgt acgcctctgg cagcagtggc gggggggcaa tgaatccgca ttggatttgc
    22741 ttctccggta taacgccgcc gacaccgaaa accttgagcc cttggccgaa atgctctacg
    22801 aacggatggt cgcgcgatac ggtccgccgt cagtcggtgc cggctatccc cccgtgcgaa
    22861 tcatcgatac gggccgtcca tgaaaacatc ggccagatgg tttgggtttg ggcggacgga
    22921 aacggggcac gtacgtcgat ccaatcaaga cgtcttcgcc gccatcaata gctgcaactt
    22981 ttggattgtg gcagacggca tgggtggaca tccggcaggc gacctggcgg ctcacattgc
    23041 cgtcgacgtg gcgacccgcc aggccaaaga gcagctgccg gcgctgagcg aggcagcgga
    23101 tgccgcagcc gatcgactag ggaatatcat catcgccgca aacgaggcca tccataacaa
    23161 agtactcgaa gaaccttcgc ttaaagggat gggcaccacc gtcgtcgccc tgacgatcac
    23221 ctcggacccg gcacccacgg cacatatcgc gcacctgggc gacagccggg cctatcgttt
    23281 tcacgaaggt atgctcacgc aactcacccg cgatcacacg ttggtcgcca tgcttttgca
    23341 gcggggattg atcgacgatg cgaccgcgcg ggcctatcct gacaggcaca tcttggccaa
    23401 agggctcggc atgggtttgg accagaagcc ggaaattact tcgatgcctc ttacaaacaa
    23461 agatctcttg atgctgtgca gcgacggctt aacgaagatg ctggacgata ccgacattgc
    23521 gtcgattctg tcgcacgcgg gaggcaatcc ccgtcgtgcc tgtcacgatc tggtcgagca
    23581 ggcactgagt cgagggggag aagacaatgt caccgtcatc gtgtgcgcct atacgacgac
    23641 aaccaaggcc ggaagtccgg ccattgacaa gacacttccg gccatgtagc ttgagactct
    23701 agccgcgcgc gccttcgcac gacatccgtc ttatcgaccg agggagacct accatgctgc
    23761 cgagtatgcg cttatttctc tgtctcatga cagccacggc cctctcgatg tcagcctgtg
    23821 tcagcatggc cgcgagcgac aacagcgcgg aagccgcggt gcgggcgctg gtgcgtgcga
    23881 atgtcgagaa ggacatccat gcgatgtcga aactgatggc acacgataca gacatcacca
    23941 gctataccat cggcggtcgg aaatatgtgg gttggtcggc ttttgaacag gacatgctgc
    24001 aagaattctc cgacgcggcg aaaattgaaa ttcccattca agaactcacg gtctggagca
    24061 agggcgacct ggcctggtat gccatggagc tcgactacat acggaccgtg atccaggggg
    24121 gcgacacgcg ccgagccgtt ctccctctgc gtgaaaccgg agtactggaa cgtcgcggcg
    24181 gacagtgggt attactctca tggcacgaat cgtttcgcaa cgtgggggga gcgatttccc
    24241 tcaacgaccg gcagcctcag agtccctccc tgaccctggc atcgacggga aacgcttctg
    24301 cctcgaccga tttgggcggc gaatgggaga tcctcgaagt tgaagacgac aaacgataca
    24361 aggccacatt ggacaaaacc ggcaacggcc cttacacgca acatggcggc cgattcgtca
    24421 cgaccaagtt ttccgatcgc ctctggcaag gcacctggca acaaccgggc aacgaccg
//
LOCUS       DATIFR010000145         4101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2200780, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000145 DATIFR010000000
VERSION     DATIFR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200780"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VLD60_08845"
     CDS             <1..250
                     /locus_tag="VLD60_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB45106.1"
                     /translation="PMHLAAAVATPVVALFGPTSATRTGPYGAGHRVLATELPCRPCF
                     NRTCRNPIQLECLTRISPEQVFAAARSQLAIHSVREHP"
     gene            247..1359
                     /locus_tag="VLD60_08850"
     CDS             247..1359
                     /locus_tag="VLD60_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB45107.1"
                     /translation="MKIFIAESSTAVGGQELAVLLHAEGLIKRGHELQLILEPGSPIC
                     AMALAKGLPVRQVPMRRSQYPSAIRTFRRMLVSEQPAILQVNSSRDSWIGGIAAKLVR
                     LRPKVIRIRHISTPLNKNLTTRLLYRWLVDMVVVTGGERTRLGLIDRDGLAADRVAAF
                     PIGLDVEYFHPAPADPDLRLELGLPHDHLLVGLISYLRTYKGHEYFIEAARQIVAKRD
                     DVTFIIVGEGPEEQPIRRLIEQLGLTSRTRMLGFRDDLLNVFRSLDVFVIPSVEGDTI
                     PQVLMQALAMGLPVVSTTVGSIPDVVIEGETGFVVPPRDARALADRILPLLDDVGLRA
                     RMGANGRALVERSYSIDKMLDKMEAVYKTLVPQRRI"
     gene            1417..3546
                     /locus_tag="VLD60_08855"
     CDS             1417..3546
                     /locus_tag="VLD60_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HSB45108.1"
                     /translation="MLKWSFKQSVVFWWLLFVVIQQVERYFLLPEVMAVEVPSADVFV
                     KTFMTGFRADLITSSIAILLICAFAGLSGTLWWGWTRARKRQVQAETGYRRSLVVAGM
                     VMALLLVVLLLVDIGYYHFNQQRLNFVFFEYVGDLMTQWTETGLRGSQAAEQTGAELH
                     DRGKWAGMVAAFLLLQATAITAWWFSFTRLVKPTLSWLGRGGVPAPNVILFMGFVGGV
                     TGFHHQGPYAIRLTDISSTAYYMLAQNPILYASDGVRAAFEARGKSDGSAKLDAMPLK
                     EAVSIAQEVLGQGAVFPYPEYPFVQERLSVPGVQFKRQANVLMILVEGLDRRFLNKTV
                     ANVRVTPFLDHLKEDSLYFEHFFANGVQTARGLFSTFCSYYPRQGTAAMKTRYMHDYV
                     CIPSLLREHGYRTEMVIGYHRDINRLHLFMSRNGLHQLFDRSDFPPGIEEIGMSASVG
                     KPDGALLDLVRTRVESLRARGNPYFLAAMTIGMHHPFAVPLTHPDIQALKAEPDGYIA
                     ALRYLDLELERVLTGMKRDGLLKDTVVLILGDHGRHEKVGQTDFEKQAGHFMTPLFLW
                     VDDSLREPGTYRPRTVMAVASQVDLLPTILGLNGVAPRLSPSMGHDLSCLLVSDCMQD
                     NMAFLSSVYDDLIGMADRDGLIFYSLRSETLRSADLDLRGAAVPVDASEPAIALRYRR
                     MLALYVSANTILNQNKIWSWKEFGNNL"
     gene            3543..>4101
                     /locus_tag="VLD60_08860"
     CDS             3543..>4101
                     /locus_tag="VLD60_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB45109.1"
                     /translation="MTISPNVSVYVIAYNDEPNMRACLESVAGWGGELIVVDSHSTDN
                     TAGIAREFTDKVHQVDFQGFGHLRNQALSFCSHEWVFSLDSDERMTPELREEIDRLLT
                     DGPVADAYFVPRKNYFLGRWIKHCGWYPDYRQPQLFRKSRFRYRQELVHESFDCEGRV
                     SHLTKPVLQYPFRDIDHYIVKQDRYS"
BASE COUNT          831 a         1088 c         1219 g          963 t
ORIGIN      
        1 gccgatgcac cttgcggccg ctgtagccac gccggtcgtg gcgttgttcg ggccgacaag
       61 tgcgacgcgg accggtccct atggggccgg ccatcgcgtt ctggctaccg agcttccttg
      121 tcggccttgt ttcaaccgga cttgtcggaa ccccattcag ttagaatgtc tcactcgtat
      181 tagtcctgag caggtctttg ccgcggcgcg aagccagttg gcaatccatt cggtgcgtga
      241 acacccatga aaatcttcat tgctgaatcc agcacagccg tggggggaca ggagcttgcg
      301 gtcctcctcc atgccgaggg gttgatcaag cgagggcatg agctccaatt gatccttgaa
      361 ccggggagtc ccatctgtgc gatggcctta gcgaaggggc tgccggtccg gcaagtcccg
      421 atgaggcggt cgcaatatcc gtccgccatt cggacgttca ggcgtatgct cgtgtcagag
      481 cagccggcaa ttcttcaagt caacagctcg cgggatagtt ggatcggagg catcgccgcc
      541 aagctcgtgc gcctcagacc caaagtgatt cgtatccgcc acatctccac tccgctgaac
      601 aagaatctga cgacacgcct gctctatcgc tggttggttg atatggtggt ggtaacggga
      661 ggagagcgga ccaggctagg cctcatcgat cgagacgggc ttgccgccga tcgggtcgcc
      721 gcctttccga tcgggctcga tgtggagtat tttcatcctg ccccagccga tcccgacctc
      781 cggctggaac taggtctccc ccatgatcac ctcctcgtcg ggttgatttc gtacttgcga
      841 acgtacaagg gacacgaata ttttattgag gcagccaggc agattgtggc gaagcgcgat
      901 gacgtcacct tcatcattgt gggagaaggt ccggaagaac agccgatccg caggctcatt
      961 gagcaactcg gcttgacctc acggaccaga atgctggggt ttcgcgacga tctgttgaac
     1021 gtgtttcgct cactggatgt ctttgtcatc ccgtcggtgg aaggggatac gatcccacaa
     1081 gtactcatgc aagcgctggc gatgggacta ccggtcgtct caacgacagt cggctcgatt
     1141 cccgacgtcg tcatcgaggg ggagacgggg tttgtggttc ctccgcgcga tgcacgagcg
     1201 ctcgccgatc gcatacttcc cttgcttgac gatgtcggac ttcgcgcccg catgggcgcc
     1261 aacggacggg ctttggtaga acggtcctat tcaatcgaca aaatgttgga taaaatggaa
     1321 gcggtctaca agaccctcgt gcctcaacgc cggatttagc gtgaccaaac gttccaccta
     1381 catgattgcc taaaaagagg aatcccagcc atacgtatgt tgaagtggtc ttttaagcaa
     1441 agcgtggtgt tctggtggct gctatttgtg gtcatccagc aagtggagcg ttacttcctg
     1501 cttcccgagg tgatggcggt cgaagtgcct tctgcggacg tgtttgtgaa gacgttcatg
     1561 acggggtttc gtgcggatct gatcacctcc agcattgcta tcttgttgat atgcgcattc
     1621 gccgggctgt cggggacgtt gtggtgggga tggacccgtg cgcgaaagcg gcaagtgcaa
     1681 gcggagacgg ggtaccgtcg ctctctggtc gtggccggta tggtgatggc actcctgttg
     1741 gtcgtccttc tcctcgtgga tatcgggtat taccacttca accaacagcg cttgaacttc
     1801 gtcttctttg aatacgtcgg agatctcatg actcaatgga cggagacggg tcttcgaggt
     1861 tctcaggctg cagagcaaac gggcgcagag cttcacgatc gtgggaaatg ggccggcatg
     1921 gtcgcggcct ttcttctcct ccaggcgacg gccatcacgg cctggtggtt ctctttcacg
     1981 cgtctggtga aaccgacgct cagttggtta ggtcgaggag gagttcccgc gcccaatgtg
     2041 attttgttca tgggattcgt ggggggggtg acggggtttc atcatcaggg accgtacgcg
     2101 attcgcctca cagatatcag cagtacggcc tattacatgt tggcgcagaa tccgatcctc
     2161 tatgcgagtg atggcgtgag agccgccttc gaggcaagag gaaaatccga tggatctgcg
     2221 aagctggatg cgatgccgtt gaaggaagcc gtgagtatcg cgcaagaggt tctgggacaa
     2281 ggtgccgtat ttccgtaccc agaataccct ttcgtgcaag agaggctctc ggtccccggc
     2341 gtccagttca aacgtcaggc caatgtgctc atgattctgg ttgaagggtt ggatcgacga
     2401 tttctcaaca agacggtcgc aaacgtccgt gtcactccct ttctcgatca tttgaaagag
     2461 gatagcctct acttcgaaca tttctttgcc aatggagtcc aaacagcgcg gggtctgttc
     2521 tcgaccttct gttcctacta ccctcggcag ggcacggcag cgatgaagac gcgctacatg
     2581 cacgattatg tttgtatccc gtcgctgctg cgtgagcatg gttatcggac agaaatggtg
     2641 atcggttacc accgggacat caaccgtcta catttgttca tgtcgcggaa cggtctccac
     2701 cagttgtttg accgtagtga cttccctccg ggtattgagg agatcgggat gtcggcgagc
     2761 gtcggcaaac cggacggggc tttgttggat ctggtgcgga cgagagtcga aagtcttcgg
     2821 gcaaggggga atccctattt tcttgcggcc atgacgatcg ggatgcacca cccgtttgca
     2881 gttccactca cgcaccctga catccaagct ctgaaggctg agccggacgg gtacatagca
     2941 gcgctgcggt acttggatct cgaactcgaa cgcgtactga ctggtatgaa gcgggatggg
     3001 cttttgaagg acacggtcgt attgattctg ggcgaccatg gccgtcatga aaaagtcgga
     3061 cagacagact ttgaaaagca ggccggacat ttcatgactc ctttgtttct ctgggtcgac
     3121 gattcattgc gggagccagg gacctatcgc cctcgaacgg tgatggccgt cgccagtcag
     3181 gtggacctgc tcccgacgat tcttggtctg aacggtgtcg ctccgcgtct ttccccgtcg
     3241 atggggcatg atctcagttg tttactggtg tcggactgca tgcaagacaa tatggcgttt
     3301 ctcagcagcg tctacgacga tctcatcgga atggccgacc gcgacggact gattttctat
     3361 tcgctgcgca gcgaaacgct gcgcagcgcc gacctggacc tgagaggagc tgcggtaccg
     3421 gtcgatgcga gcgagccggc gatcgcgctt cgctacagac ggatgttggc gctgtatgtc
     3481 agtgccaaca cgatcctcaa tcaaaacaag atctggtcct ggaaagagtt cgggaacaat
     3541 ctgtgacaat cagtcccaac gtgtccgtct atgtcatcgc ctacaacgat gaaccgaata
     3601 tgcgcgcctg ccttgagtcc gtggccggtt ggggtggcga gctgatcgtc gtcgattcgc
     3661 acagcacgga taacacagcg gggatcgcgc gcgagttcac cgataaggtc catcaagtcg
     3721 atttccaagg attcggacat cttcgaaatc aagcgctgtc attctgttcg cacgagtggg
     3781 tgtttagttt ggatagcgat gagcggatga caccggaact gcgggaagaa attgaccgac
     3841 tgttgacaga tggacctgtt gccgacgcgt attttgttcc acggaagaat tatttcttgg
     3901 ggcgatggat caagcattgc ggatggtatc ctgactatcg gcagccgcag ctctttcgaa
     3961 agagccgctt tcgttatcgc caagaactcg ttcacgagag tttcgattgt gagggacgag
     4021 tcagccatct caccaagccg gtgctccagt atccctttcg ggacatcgac cactacattg
     4081 tcaagcagga tcgatactcg g
//
LOCUS       DATIFR010000146        22343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_671066, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000146 DATIFR010000000
VERSION     DATIFR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671066"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VLD60_08865"
     CDS             <1..333
                     /locus_tag="VLD60_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HSB45110.1"
                     /translation="MDADPFAVLDREGVGSLMKQAIAAGRTTRPGIKLGICGEHGGDP
                     SSVEFCHQLGLDYVSCSPYRVAIARLAAAHAALAGADAKKSARKSSGSTSKSKSSARR
                     SRAARKRR"
     gene            330..1448
                     /gene="ribD"
                     /locus_tag="VLD60_08870"
     CDS             330..1448
                     /gene="ribD"
                     /locus_tag="VLD60_08870"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSB45111.1"
                     /translation="MTTRLQDLQYMTLALRLAAKGRGKTSPNPMVGAVVVSNGRIIGQ
                     GYHHGPGQPHAEILALRDAGRRANGATLFVTLEPCSHLNKRTPPCVPAVLRSGVRRVV
                     VAMVDPNPSVKGRGIALLRRAGLSVTVGIGREEAERLNLAYCHWSRTRRPYVTLKAGM
                     TLDGKIATATGESQWITGPAARKDVHRLRAQVNAVLTGVGTVRADNPSLTARTGARLK
                     TLASRQPLRIVVDSKLRIPSNAQVLSRQQEARTIVATTGSAPAARLRAIQKRGVETIT
                     LPAIRGQVSLPALLKELGRRGVTALLVEGGSEVNAAMLKAKLVDHVRLYIAPALLGGG
                     DAKGIIGGRSPARLVQTMKLRDVRIRKVGSDLLVEGDL"
     gene            1445..2725
                     /locus_tag="VLD60_08875"
     CDS             1445..2725
                     /locus_tag="VLD60_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45112.1"
                     /translation="MTRGLLACLLLLALTAWVEIGWAASAPDDLAALVFQYLDTGDSD
                     EADRLLQTILSRADASIESVSRIMQTERVYQSQPIGTLPNEQIQIQGRSYPLALSVPL
                     TYQPAKGYGLVVCLHGAGFTGDAYLERWQARLGEEYLLACPTYASGAWFTRTAEDLVL
                     TAIQQVQRRYHVDPDRIFLTGMSNGGIGTWLIGMHHAPLFAGIAPMASGLDDVLMPFL
                     ANLRNTPVYIIHGAKDRVMPVELSRSISRELTALGYPHVYREHQREHPMAGGHYFPRE
                     ELPELVAWFNAQRRNPFPTTIRLVREASHFQPFGWVRIDGTDEIAAFSEDLINKRDAL
                     TKSKQYAKLEASLVAPNRIEVATKRVRRYSLFLNDRLIDVSKPLTVSTDGQVSFEGMV
                     VPSLDTLLRQGRVRQDPRQLYPIHVRISVQKQGS"
     gene            2761..3537
                     /locus_tag="VLD60_08880"
     CDS             2761..3537
                     /locus_tag="VLD60_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45113.1"
                     /translation="MVWPLLLALSVAALPGFVSAESCVLPSQHAGMSFPVEKVDSLWA
                     CRLHSIISNYSTANKVGPIRAALSESMYRYLLDRPPLAAALIKRLDLAPYKSEQRGPG
                     RYWGNDGEGTEGTVELVYQDRTSRIYYLEGTHHSRLLPNMRGKAVVLLRMHPVNETSG
                     AEAMDSTMVAYLKLDNRILSGLVSLLRPLIGGTITRKLVKGVEVVNRLGIEMRQHPDR
                     VLAEAMDSPPLPDVDVVFLKQALTDLQSSGRTHQKGQLTK"
     gene            3534..4703
                     /locus_tag="VLD60_08885"
     CDS             3534..4703
                     /locus_tag="VLD60_08885"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB45114.1"
                     /translation="MTSTRSFMLICTVGIFCFISYNMVRMPVLALFAESLGAGPERIG
                     LIVSVSTLTGVFLKLPSGALSDIYGRRFLLRIGVVAFGLPPFFYPFIHNLDALTALRF
                     FHGLATAIFAPSALATVAELYRERRGAALGTYTACTQSGSLLGPFIGGYLVYAAGFSS
                     AFVTAGVFGCTAIVFFYSLHLDIATPHAKEKGLMTVLAEMWKGFVVVARNRKVLITSS
                     TDAAKMIANGALMAFLPLYGVSVGLNPGEVGLLFTIQAFTSFFSKPIMGRVSDRVGRQ
                     PLIMMGLLICASTFVCIPHVSIFLVLLMLSAGFGFGEAVVSSSSSALVADSSEFKTLG
                     AGMGMQGTIMDIGHASGPLLAGVLIANMSYAGAFGVIAGIQVLAAGVFWFTMRQR"
     gene            4845..5498
                     /locus_tag="VLD60_08890"
     CDS             4845..5498
                     /locus_tag="VLD60_08890"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSB45115.1"
                     /translation="MFTGIVEEMGAVTSLDRTLAGTRMTILASTVMGDLKIGDSVSIN
                     GTCLTVVTKGEHDFVVEVSPETLSVTTMGKLAPGAPVNLERAMKLNERIGGHLVAGHV
                     DGVGTIRSRHQEGNAIFFTIEAPSEILRYCVVKGSITVDGISLTINDVADHSFSVAII
                     PHTAKVTTLGLKQVNDTVNLESDLIGKYVERLLQERSQLPKTTPIIDKDYLQKHGLL"
     gene            complement(5548..5769)
                     /locus_tag="VLD60_08895"
     CDS             complement(5548..5769)
                     /locus_tag="VLD60_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45116.1"
                     /translation="MKGIRKPGEPIYLRRHMVALALAVGLPILLLQLYKIYVGPIGFG
                     VQMLIGVVISILAGIVLYFAYRSSAQNQP"
     gene            complement(5818..6288)
                     /locus_tag="VLD60_08900"
     CDS             complement(5818..6288)
                     /locus_tag="VLD60_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB45117.1"
                     /translation="MVKQVGHTTMVVIGAVALVLTAGIPGQGLAADKEKSAKKPKAAP
                     EAKTSKPKADKPKFQSAETAGKGKTCFGTAPKIEQLVPDEGKPGDKVTIKGSLFGSPD
                     CLRSVSFGPGHAATFTMQNDNTITATVPTGGRKGLAIVTVTTASGEDSKPFLVK"
     gene            complement(6343..8232)
                     /gene="acs"
                     /locus_tag="VLD60_08905"
     CDS             complement(6343..8232)
                     /gene="acs"
                     /locus_tag="VLD60_08905"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSB45118.1"
                     /translation="MTDNIETLLKESRIYQPSDKTKSAAYIKDYETEYKKSIADPEGF
                     WSGMAKELDWFSPWSKVLEWNYPWAKWFVGATCNITYNCLDRHVKTWRKNKVALIWVG
                     ENDQERIFTYAELYRQVNRCANALKKLGIKKGDRVTIYLPKIPEQVVAMLACARLGII
                     HSVVYSGFSAPALASRVNDAEARLVITADVGFDRGKVINLKPIVDDALKSCSSVEHVV
                     VVRRQSPGPTLTAPREIDWNDWVKNEKALCEAEQLDAETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYATTKYVFDMKDDDVYFCVADPGWVTGHSYIVYGPLLNGATILTAEGKP
                     DYPNPGRWWDLIQRYGVSIFYSTPTAIRLLMRHGEDWPKKYDLSSLRILGSVGEPINP
                     EAWEWFHRAAGGDKPIMDTWWQTETGSILITPLPTVPLKPGSATRPFLGIEADVVDRA
                     GNSLPANQGGFAVIKKPWPSMMRTIYKDPERYKTYWNTIPNCYMAGDICHKDADGYLW
                     FMGRADDVIKVAGNRLGTAEVESALVSHHAVAEAAVIGKPHKTVGESIKAFIILKQGE
                     QESSELIQSIKDQVKHELGKIAVPAEIEIVTSLPKTRSGKIMRRVLKAKELGQDPGDI
                     STIEE"
     gene            8432..9826
                     /locus_tag="VLD60_08910"
     CDS             8432..9826
                     /locus_tag="VLD60_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSB45119.1"
                     /translation="MMNPTSRQSFRARFLAILVVVFWGIGGAATVWAQVGKPEGLYYK
                     SWAVVIGVENYLLAPKIPGVIDDAKAVAQTFRKLGFDEVVELYDKDASSRRLQQTLME
                     FLPRKVGRQDRLVIYFAGHTGITQGLSGKDLGYLVPWDAQIANVSKSVTFEQLKEFSH
                     RSASKHTLLLFDAAVRGWEVSAAQTLSLEGRSAPEDDTEKRAVQVLTAGNKGESLARK
                     QDHSLFVQALVKGLSGEADLDKNGWLMASEVGRFVTQQIQDQTKGAQHPIFEQLEGDG
                     DTIIVEGRKAAFRLGGEPQSPAERQQAAKMHYEQAFALLQTGKAPDEALERLNKALEY
                     DPTFGDAYVLKSYVRLEVLPNLDEALAAANAAVKYAPKNPDSQYTLGLIYEKRGQYAE
                     AERAMREALVVNPSYLDVYFSLGTMYADEMKDQQKAVAAFERYLELGGNHTRAREAVA
                     QSRDAGTGTPPSKP"
     gene            complement(10138..10308)
                     /locus_tag="VLD60_08915"
     CDS             complement(10138..10308)
                     /locus_tag="VLD60_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45120.1"
                     /translation="MADVQIDDGTIKILNLDIQDPKAAAVLAEYPQVRWAEITRRALK
                     IGLGYLKGGGKD"
     gene            10480..11046
                     /locus_tag="VLD60_08920"
     CDS             10480..11046
                     /locus_tag="VLD60_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45121.1"
                     /translation="MNQPQGLMAKVTSGGIALLLLIGLAACGGPPKWVKQGSGAFNDK
                     DSKAFYGVGSVVGVRNEPLAWDTAENRARAEIAKTFETYTGYLMRDYAASTTAGDFTR
                     NTEEQNVERAIKTVTTATLSGVRPIERYKDDKTGTYYVLTKLNLEEMKNNLEQAKELN
                     SQVRDFVRKNADKLFDRLEKEEEKRANR"
     gene            11076..11705
                     /locus_tag="VLD60_08925"
     CDS             11076..11705
                     /locus_tag="VLD60_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HSB45122.1"
                     /translation="MEGSVVRVRSGVSVAVVIGVALAALSGCAQETKVTRVDTGVVTD
                     LSGRWNDTDSRMVAEAMVKEALEYPWLGNFSQSKHRQPVVVVGTVLNNSHEHINVQTF
                     VTDLERELTNSQKVTFVAGKGEREEVRDERKEQAMYAREDTQKAPGKEIGADYMMKGT
                     IATILDEADGTKAMFYQVDLQMVDLESNAKVWFGQKKIKKVIEKKRSVF"
     gene            11877..13217
                     /locus_tag="VLD60_08930"
     CDS             11877..13217
                     /locus_tag="VLD60_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45123.1"
                     /translation="MQNFQQADAIIQKAENEYGSKSRVLYGMDRGMTLQLAGEYQQSN
                     AVLEEAEDEVDRLYTRVVRTETLAFMTNDNALPYEGDPYEQVMINVLKALNYASLNQW
                     QDALVEARRIDHRLNVLSDRVQDKGAYRDDGFARYLSGILYESAGDVNNAFIAYRKAY
                     EIFESTSAWSRSRVPAQLKADLLRTADALHLTSELTEYQRAFPDTSWQSLEAQQQLAQ
                     VVVISYNGRAPRKEDQFLDLPISLDALQLVLLNRGFYQSNRHQNRAADSILYGLNGRV
                     VRVAVPRLVSQKTQVPVDTVNLIPESGVPVTVRTELVQSVTALADKALSERITAITVK
                     ALARAATKFSLAEGATRGAQQAAGRDAAPWVGLVVGLLTKGLAVASEESDKRSWRTLP
                     DEIHLARTWVTPGHYKVQGAALTGTNNSGNLAPPERVLSLHAGETAVLIQRVIP"
     gene            13229..14377
                     /locus_tag="VLD60_08935"
     CDS             13229..14377
                     /locus_tag="VLD60_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HSB45124.1"
                     /translation="MTGTIGAGIILGTALLWGCTWFGGQAKPAWIDGVSPDYPSAQYL
                     TGVGQGDNRAVAEDQAYAALARIFKAEVSAQSKDWESYLVVEERGHSKDERRLTLDNL
                     TRVSTDKVLENVKVVDRWYDPKKGLHYALAGMHRPQAETAFMERIAELDQSIHSDVEE
                     AHHSADKLAKVRGLRRAARNLVLRETYNADLRVIRPSGQGTAAAYRVQELTRELEDFL
                     SSNLVLAVTVTGDQAEPVQRALIEGLIKEGLQVSSRTTGTEQQTGGSGVYPAPELLVR
                     GMVRVWPIDVRDPQFTYVRWCSDFEVVDVTSQRVVGALSKGGKEGHLTEREATAKVVR
                     VMQQDMSADVAKAIAAHVFGETELPAQAIRPAGCPREGSGSKPATITH"
     gene            14400..14663
                     /locus_tag="VLD60_08940"
     CDS             14400..14663
                     /locus_tag="VLD60_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45125.1"
                     /translation="MSRPNQAGRAPRSRGRGLTATQQRVPSRLAKRRLGERLKNDTAL
                     FNKGNLADKLRKEGYEEVPLDELQDRLSKMPGPLAAVILKGRI"
     gene            14667..15110
                     /locus_tag="VLD60_08945"
     CDS             14667..15110
                     /locus_tag="VLD60_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB45126.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIVNKLMVKRGKVAIIPTWTVTE
                     LYSAFSNRAQQGELTRDDCYSVIHKFERESEEGMFQFIAPTMQTYLATKELVMEYPFL
                     RTLQVMHLALASELKPLRLTVVSADIQLLAASKTAGLHIINPEED"
     gene            complement(15164..15697)
                     /locus_tag="VLD60_08950"
     CDS             complement(15164..15697)
                     /locus_tag="VLD60_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45127.1"
                     /translation="MRLYFTVSTAPFLIGICLCVGCGVLTIERSVAQVMGDAAEMDRL
                     RVKAEEAIGNDDPEGAAMNMGRAALMAKRLGLLNKDDNVAIRLYRGAESLFRSQEHGY
                     RAMALFRRAGGQLPASSGVCGSLALAQGNVQHALSDLAQEILSPPPLAERWKRWRETA
                     DDWVIVVASMITDYQCP"
     gene            complement(15702..16778)
                     /locus_tag="VLD60_08955"
     CDS             complement(15702..16778)
                     /locus_tag="VLD60_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB45128.1"
                     /translation="MSLARIAISCLLVSLVVVSTAWARRDALTGQQKEQLQKIDRILL
                     DVLALTDQGQTDASPFRDLTAKRFGELGYTIVTDPSQPYDVLFRVKCEQRKVWEGTMA
                     SGGDADLPDSPMRTWKGPACQLTYLLDGKSTQWKKEVRTAFADAIAAAAQAKASDAGP
                     YALDHLKQRLEEYDFPVFVTADWGQDSRLLKVLAEGNPPTARRVRIIQSLGEIFSTQA
                     VPQLQAALKDPNPDVSKAAAIALGNIGHKDSIASLISMLKTGSPDQKVVAAKALGKVG
                     ALHGDASIIPPLLEALNTDDLVLKTEVVWALGQLPDRRAYEPLVALQRSLRNVRTDRG
                     SQEGKLWEAVNYSIKQLDGFDQIN"
     gene            16756..17673
                     /gene="proC"
                     /locus_tag="VLD60_08960"
     CDS             16756..17673
                     /gene="proC"
                     /locus_tag="VLD60_08960"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSB45129.1"
                     /translation="MAIRASDIDASLYRTGCNPCKLQGLWSDHYNRRMLHDRRLACIG
                     AGNMAEAIVAGLLKGKHVFPAQLMASDIRADRRADISRKYQFATAEKNADVAAWADVI
                     LLAVEPQVLDEVLQEIAPVIGEGTLVVSVAAGYPIARLKRYLPGTKRIVRAMPNTPST
                     IREGVTAVAYAAGLVDDDVRTARALFEPIGRVVEVNEGALDAVTGLSGSGPAYVYVMI
                     EALADGGVKMGLPRETAQLLAAQTVAGAARMVLESREHPGALKDRVASPGGTTIAGLH
                     ELERGCLRSTLMSAVEAATKRSSELGLVT"
     gene            17690..17884
                     /locus_tag="VLD60_08965"
     CDS             17690..17884
                     /locus_tag="VLD60_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45130.1"
                     /translation="MQYWVKVVFVDNQELMVKDAVRHTISDDMEVLEVDTAKEVFIIP
                     MKQLKYLACDATVFAAKKPG"
     gene            complement(17923..18756)
                     /locus_tag="VLD60_08970"
     CDS             complement(17923..18756)
                     /locus_tag="VLD60_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HSB45131.1"
                     /translation="MTRFVVFTDLDGSLLDSETYSFEMATPALDALRMRNIPLILVSS
                     KTRAEMEPLRRRLNHLDPFIVENGAAVFVRQGLFDFPLERVRTKSSYDVIELGLPYHM
                     LRDVLKQIEDAVETPLRGFGDLSVDEVMHLTGLSRTDATLAKMREYDEPFLLEGPQDL
                     IEEVCWQIVSRGLRWTKGGRFFHLTGDNDKGEAAELLLRCYQRQLRLLEQPDRIETVG
                     IGDSVNDAPLLAMVDHPILVQKPDGSYDPDIQIPGLIRAPGIGPAGLNEAVLALLDRV
                     A"
     gene            complement(18834..19541)
                     /locus_tag="VLD60_08975"
     CDS             complement(18834..19541)
                     /locus_tag="VLD60_08975"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45132.1"
                     /translation="MNELFEQALMEPLQLLGRHALAILPNVFAMGILLLGGILTAWLL
                     SVSVERVLRVIGFDHLSNRLGTTTALLRAGIKTDPSRLIGRTLYWAVVLVASVAALGA
                     LNLAPINQFAQSLLAYVPHLITAGVIVLAGYVCSNFAYQAILIAAVNAGLPPARLIAN
                     CSRWGLQLLAGAMALEQLGIAQHIVVVGFGITWGGVVLATAIAFGLGATDLAKEFLEQ
                     RVIRRNKSTVTDDLHHW"
     gene            complement(19525..20868)
                     /locus_tag="VLD60_08980"
     CDS             complement(19525..20868)
                     /locus_tag="VLD60_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB45133.1"
                     /translation="MSTNHTPLAPATESRLREIGSADILVGIPSYNNADTVGHVVRAV
                     SAGLAKYFPDEPAVLVNSDGGSSDETAAIVARTVIDHHHTLIADRQSVLHRIVTPYHG
                     IPGKGSAFRTIFEIARQLNAKACAVVDADLRSISPEWIEMLLRPLREGGYDYVAPFYL
                     RHKYDGTITNSLAYPLTRSLYGKRIRQPIGGEFGFSGALAKHYLDQHVWESEVARFGI
                     DIWMTTEAIASGARVCQSFLGAKIHNPKDPAADLSAMLRQVVGSLFASMEQHATIWSG
                     IEGSQPVPLFGFPYEVGVEPVNVDVEGMVSHFQQGLRDLEPIWKQILADLTFEQLYDL
                     NRQAPTCSRVPDELWVQIVYDAAVAYHEQILPREHLLKSLTPLYLGRTASFVLATQGL
                     TSAEAEQTIETLCQSFEQNKPYLLARWEEMDRQPAMPRLQSRTVTDAGGSHERIV"
     gene            complement(20865..22109)
                     /locus_tag="VLD60_08985"
     CDS             complement(20865..22109)
                     /locus_tag="VLD60_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HSB45134.1"
                     /translation="MSDFYQNGVVTVLHRLGDPNVDQLERELSRYGNIHPIALVLPSL
                     YAELSRPALKRIVETLKGVSYLNEIVISLDRASALEFRLAKQYFSQLPQRVRLIWNDG
                     ARIQSILNVLVEKNLDIGLQGKGRGCWMAFGYVLARNQSQVIALHDCDITSYNREYLA
                     RLCYPIANPNLGYEFCKGYYSRVTDRLHGRVTRLFITPLIRSLKLLVGAHPLLTFLDS
                     FRYPLAGEFAMVRDLAWTNRIPGDWGLEIGVLAEVYRNCALRRICQADIADAYDHKHQ
                     ALSNENPDAGLLKMCVDITKSLFRNLASEGVVLSDSVLKTLRATYLQAAQEAISRYEN
                     DAAINSLAFDRHEERTAVEVFLKGMKLATDGFLDDPLGVPMISNWSRVAHAVPDIFER
                     MTEAVEADHVWDPTSTPVGTPP"
BASE COUNT         4731 a         6065 c         6829 g         4718 t
ORIGIN      
        1 atggatgcgg atccctttgc cgttctcgat cgggaaggtg ttggatcgct gatgaagcaa
       61 gcgattgcag ccggccgtac gacgcgcccc ggcatcaagc tcggtatttg cggcgagcac
      121 gggggtgatc ccagttccgt ggagttctgt catcagctcg gcctggacta tgtgagttgc
      181 tcgccctacc gtgtggccat tgcaagactc gcggcggccc atgcggcgtt agcgggagcc
      241 gatgcgaaga agtcggctag aaaatcgtcc ggatcgacgt ccaaatcgaa gtcatccgca
      301 cggcgctcgc gcgcggcgcg caagagacgg tgaccacccg cttacaggac cttcagtaca
      361 tgaccctggc gcttcgcctt gctgcgaagg gccggggaaa gaccagcccg aatccgatgg
      421 tcggagctgt ggttgtctcc aacggccgga tcatcggaca gggctatcac cacgggcctg
      481 gccaacctca cgcagaaatt ctcgcgctca gagacgccgg tcgtcgcgcc aatggcgcga
      541 cactctttgt tacgctcgaa ccctgcagcc atctgaacaa acgtacacca ccctgtgtgc
      601 cggcggtgct ccgatccgga gttcgtcgtg tggtcgtggc catggtggat cccaacccat
      661 cggtaaaagg aagaggcatc gccttgcttc gtcgagccgg attgagcgtg acggttggca
      721 tcggtcggga ggaggcagag cggctgaatc tggcgtattg tcattggagt cggaccaggc
      781 gcccgtatgt gacgttgaaa gcggggatga cgctcgacgg caagattgcc acggcaacgg
      841 gggaatcgca gtggattacc ggtccggcgg caaggaagga cgtgcaccgg ttgcgggctc
      901 aggttaatgc ggtcctgacc ggggtcggca cagtacgggc agacaatcca tcgctgacgg
      961 ccagaaccgg cgcaagacta aagacgttgg catcgcggca gccgctccgc atcgttgtcg
     1021 acagcaagct gcgcattccg tccaacgcgc aggtcctttc acggcagcag gaagccagga
     1081 cgattgtggc cacgaccggc tcggctccag cggctcgact acgggcgatc cagaaaagag
     1141 gcgtcgagac gatcacgctt ccagccattc gcgggcaagt gtcgttaccc gcgttgctca
     1201 aagaactggg ccggcgcggc gtcaccgctc tccttgtcga aggcggcagt gaggtaaacg
     1261 cggcgatgct gaaggccaaa ctggtggatc atgtgcgcct gtatatcgcg ccggcgctac
     1321 tgggcggggg agatgcgaag gggatcattg gcggcaggag ccccgcccgt ctggtccaga
     1381 ccatgaagct tcgggatgtc cggatacgca aggtcggaag cgatctcctc gtggaaggag
     1441 atttgtgacg cggggcttac tggcctgtct tcttctgctg gctcttacgg cctgggttga
     1501 aatcggctgg gcggcttcag cgccggatga tcttgccgcg ctcgtgtttc agtaccttga
     1561 tacgggcgac agcgacgaag ctgatcgcct gttgcagacc attctttccc gcgcggatgc
     1621 atcgatcgaa tcggtttctc gcatcatgca gaccgagcgg gtctatcaga gtcagccgat
     1681 cgggacactg cctaatgaac agattcaaat ccaagggcgc agctatccgc ttgcgctctc
     1741 tgttcctctg acctatcaac cggcgaaagg gtatggattg gttgtctgct tgcacggggc
     1801 cggcttcacc ggagatgcct atctcgaacg gtggcaggcg cggctgggtg aggagtatct
     1861 gctggcctgt ccgacctacg cgtcgggagc ctggttcacg agaaccgcgg aggatcttgt
     1921 cctgacggca atccagcaag tacaacgtcg gtaccatgtt gacccggacc ggatctttct
     1981 cacgggaatg tcgaatggcg ggatcgggac ctggctgatc gggatgcatc atgcgccgtt
     2041 gttcgcagga atcgcgccga tggcgagcgg actggacgat gtgctcatgc cgttcctcgc
     2101 aaatctccgg aatacaccgg tctacatcat tcacggcgca aaagatcgcg tgatgccggt
     2161 ggaattgagc cgatccattt cccgggaact cacggcgttg ggctacccgc atgtctatcg
     2221 cgagcatcag cgtgaacatc cgatggcggg cgggcactac tttccgcggg aagagctgcc
     2281 ggaactcgtc gcctggttca acgcgcaacg ccggaatccc tttcctacga cgatccggct
     2341 cgttcgcgag gcaagccact ttcagccgtt cggatgggtc cgcatcgatg gcaccgatga
     2401 gatcgccgcc ttttcagaag atctgatcaa taaacgtgat gcactgacca aaagcaaaca
     2461 gtatgcaaag cttgaagcgt cgctggtagc gccgaaccgg atcgaagtgg cgacgaaacg
     2521 ggtgcggcgc tatagccttt tcttgaacga tcggctcatc gacgtgtcaa agcctctcac
     2581 ggtctccacg gatggacagg tctcgttcga agggatggtc gtgccctcgt tggatacctt
     2641 gctgcggcag ggaagggtga gacaggatcc tcgccaactc tatcctatcc acgtgaggat
     2701 ttcggttcag aagcaaggct catgagcctg ggggagcggc tctttcaaag gagaggcccg
     2761 atggtctggc ccctattgct cgcactcagc gtcgcggctc taccgggttt tgtgtcggcg
     2821 gaatcctgcg ttctcccgtc tcaacatgct gggatgagtt ttcctgtcga gaaggtcgat
     2881 tccctgtggg cctgccgact gcattccatc atcagtaact attcgacggc gaataaggta
     2941 gggccgattc gggcggcctt atccgagtcg atgtatcgat atctgttgga tcgtcccccg
     3001 ctggcggcgg ccctgatcaa gcgcctcgat ctggctccat acaaatcgga gcaacgaggt
     3061 cctggccgtt attgggggaa cgatggtgaa ggaaccgaag gcacggtgga gcttgtctat
     3121 caggatcgca cgtcacggat ttattatctc gaggggactc atcacagccg gttactccca
     3181 aacatgaggg ggaaggccgt agtgctgctc aggatgcacc cagtcaatga aacgagcgga
     3241 gccgaggcca tggacagcac aatggttgcg tatctgaagc tggataatcg catcctgtcc
     3301 ggtctcgtat cgctgctccg gccgctgatc ggcgggacga tcacacggaa gctcgtgaaa
     3361 ggtgtggagg tcgtcaaccg cttagggata gagatgcgcc aacatcccga ccgggtgctg
     3421 gctgaggcca tggattcccc gccgctgccg gatgtcgacg tcgtgttctt gaaacaggca
     3481 ttgacggatc ttcagagctc cggcaggacc catcaaaagg gccagctgac caaatgacct
     3541 ccacgcgcag cttcatgctg atctgtacgg tcggcatctt ttgtttcatc agctacaata
     3601 tggtgcgcat gcctgtcctg gcgctgtttg cggaatcgct gggtgccggg cctgaacgga
     3661 tcgggcttat cgtgtcggtt tcgaccctga ccggagtctt cctcaagctg ccgtccggag
     3721 ctctgtccga tatttacggg cgaagattcc ttttgcgcat tggagttgtg gccttcggcc
     3781 tgccgccgtt tttttatccc ttcattcaca atctcgacgc gctgaccgct ctgcgattct
     3841 ttcacggtct cgccaccgcc attttcgctc ccagtgcgct agcgaccgtg gcggaactct
     3901 atcgagaacg gcgcggtgcc gcactgggga cctatacggc ctgtacgcaa tcgggatccc
     3961 tgcttggtcc ctttatcggc ggctatcttg tctatgccgc gggattctct tccgccttcg
     4021 tcacggccgg ggtctttggc tgcacggcaa tcgtattttt ctacagtctg catctcgata
     4081 tcgcaacgcc acacgccaag gaaaagggat tgatgacggt gctggccgag atgtggaagg
     4141 ggtttgtcgt cgtcgcgcgg aaccgaaagg tcttgattac gagttccacc gatgcggcga
     4201 agatgatcgc caatggcgcc ttgatggcgt tcctgccgct ctatggtgtg tcagtgggac
     4261 tgaatccggg agaggtcggc ctcttgttca cgattcaagc gttcacctca ttcttctcga
     4321 agccgataat ggggcgagtc tccgatcggg ttgggcgaca gccactgatc atgatggggc
     4381 tcctcatctg tgcctcgacc tttgtctgta tcccgcacgt ttcgatattc cttgtgctgc
     4441 ttatgctctc ggccggattt ggcttcggtg aggccgtcgt gtcgtcgtct tcttcggctc
     4501 tggttgcgga cagttccgag ttcaagacgc tcggggcggg tatgggcatg caggggacta
     4561 ttatggatat cggccacgcc agcggaccgt tgctggcagg tgtgctcatc gcaaacatga
     4621 gctatgcggg agccttcggc gtgattgcgg gtatccaggt gcttgcagca ggagtgttct
     4681 ggttcacgat gagacagaga tagggcaaaa caattataat gtggccggat gcgtgacttt
     4741 tcgacagaag acatagtggt cggcgttgtg gcgacagtcg gagtcgtcgt cttgctcgtc
     4801 ctgttcgtgt atgtggtgaa acagatcatc ttgaggaagg actgatgttt accggaatcg
     4861 ttgaagaaat gggtgccgtc acgtcattgg acaggaccct tgccgggacg aggatgacaa
     4921 tcctcgcgtc gacggtgatg ggcgatctca agatcggcga cagtgtcagc atcaatggca
     4981 cgtgcctgac ggtagtgacc aagggagagc atgactttgt cgtggaagtc tcgccggaaa
     5041 ctctctccgt gacgaccatg ggaaagctgg ctcccggagc tccggtcaat cttgagcggg
     5101 ccatgaaact caacgagcgg attggcgggc atctcgtggc cggtcacgtg gacggtgtgg
     5161 gaactatccg cagccggcac caggaaggga atgcgatctt ctttacgatc gaagcgccct
     5221 cagaaattct ccggtattgc gttgtcaagg gatccatcac ggttgatggt atcagcctga
     5281 cgatcaacga cgtggccgac cattccttca gtgtcgcgat cattccgcac acggccaaag
     5341 tgaccacgct tggcctcaag caagtcaacg atacggtcaa tctggaatcc gacctcatcg
     5401 gcaagtacgt cgaacggctg ctccaggagc gcagccagct gccgaagacc acacccatca
     5461 tcgacaagga ttatcttcaa aaacacggtc ttctctagtg tgagtccaag ctttctcgtg
     5521 ccgaacaatc gctcgccggg cagttgccta cggctgattc tgcgccgacg accggtaggc
     5581 gaagtagagg acgattccgg cgaggatcga gatgacgacc ccaatcaaca tctgcacgcc
     5641 aaaaccgatc gggcccacgt agatcttata tagttgcaag aggagtatcg gcaggcccac
     5701 cgccagggcg agcgccacca tgtggcgtct cagatagatc ggctcgccag gttttctgat
     5761 ccccttcata gacgagagac tgatggacca gaagcgttcc cctggccctc gtcgaattta
     5821 cttcactaag aacggcttgg agtcttcgcc tgaagccgtg gtgaccgtca cgatggcaag
     5881 tcccttgcga ccgccagtgg gtacggtggc agtaatcgtg ttgtcgtttt gcatcgtaaa
     5941 ggtcgcggcg tgacccggac cgaaagagac gctgcgcagg cagtccggtg aaccgaacag
     6001 cgagcccttg atcgtgacct tgtcgcctgg ttttccctcg tccggcacga gctgttcgat
     6061 ttttggcgcg gttccgaaac aggtcttccc tttaccggca gtttccgcag actgaaactt
     6121 tggtttgtcc gctttgggct tggaggtctt cgcttcagga gcagcttttg gcttcttggc
     6181 cgacttctcc ttgtctgccg caagcccctg tcccggaatg cctgccgtga gcacaagggc
     6241 aactgcacca atgactacca tggtcgtgtg acccacttgc ttcaccatgc cggcctcctt
     6301 taggcagcgc aatcatcgct gcatgatatt gtgtggggcg cgttactcct cgatcgtcga
     6361 gatgtctcct gggtcctgtc ccaactcctt tgctttgagt acccgccgca tgatcttgcc
     6421 cgagcgcgtc tttggaagcg atgtaacgat ctctatttcg gccggcacgg cgattttccc
     6481 cagttcatgt tttacctggt ctttgatcga ctgaatcaat tccgagcttt cctgttcacc
     6541 ctgtttcaga atgataaaag ccttgatgga ctcgccgacg gttttgtgcg gcttgccgat
     6601 gacggcggct tccgcgaccg catggtgact caccagcgcg ctttcaactt cagcagtgcc
     6661 gagacggttg cccgctactt taatcacatc gtcagcccgg cccatgaacc acaagtagcc
     6721 gtcggcatct ttgtggcaga tgtcaccggc catgtagcaa ttggggattg tattccagta
     6781 ggtcttgtag cgctcggggt ctttatagat ggtacgcatc atcgagggcc agggtttctt
     6841 gatgaccgca aagccgcctt gattcgccgg caggctgttg cctgctcgat cgaccacgtc
     6901 ggcctcgatg cccaagaacg gtctggtcgc agagcccggt ttcaatggaa cggtcggcaa
     6961 cggcgtgatc aggatcgagc cggtttccgt ttgccaccag gtatccatga tcggtttgtc
     7021 gccgcctgct gcacgatgaa accattccca ggcttccgga ttgatcggtt cgccgacact
     7081 accgaggatg cgcaagctgg acagatcata tttcttgggc cagtcctcgc cgtggcgcat
     7141 gagaagtcgg atggccgtcg gggtgctgta gaaaatggag acgccatagc gttggatcaa
     7201 gtcccaccac cggcccggat tcggataatc cggcttgcct tctgccgtca gaatggtcgc
     7261 gccgttcaac aagggtccat agacgatgta actgtgcccg gtcacccaac ctggatcggc
     7321 gacgcagaaa tagacgtcgt cgtccttcat gtcgaagacg tacttggttg tggcgtaggt
     7381 cccgaccatg tacccgccgt gaacgtgcac gacacccttc ggtttgccgg tggtaccgga
     7441 agtgtagagg atatagagcg gcgtttctgc atcgagctgc tctgcttcgc acagggcctt
     7501 ctcgttcttg acccagtcat tccagtcgat ctctcttgga gccgtgaggg tcggaccggg
     7561 gctctgacgg cggacgacga ccacatgctc gacgctgctg cagcttttca gtgcgtcatc
     7621 gacgataggc ttcaaattga tgactttccc gcgatcgaat ccgacatcgg ccgtaatgac
     7681 gagccttgct tcagcatcgt tgacgcggct ggccagggcc ggcgcgctga atcctgaata
     7741 gacgacgctg tgaatgatgc caaggcgggc gcaagcgagc atggcgacga cctgctcggg
     7801 gatttttggg agataaatgg taacgcgatc gcctttcttg atcccgagtt ttttcaacgc
     7861 attggcgcag cgattgacct gccggtagag ttcggcatag gtgaagatgc gttcttgatc
     7921 gttctctcca acccagataa gtgccacctt gttcttgcgc cacgtcttga cgtgccggtc
     7981 gaggcagttg taggtgatgt tgcaggtggc gccgacaaac catttggccc aaggatagtt
     8041 ccactccaga accttgctcc agggagaaaa ccagtccaat tccttcgcca tgccgctcca
     8101 aaacccctct gggtcggcga tggatttctt gtattcggtc tcgtagtcct taatataggc
     8161 ggccgatttt gtcttatcgg atggttggta gatacggctt tcttttaaaa gggtctcgat
     8221 attgtccgtc atagtggaca cgctccttga aaaccaaaca gatggccggt tgtcctgaga
     8281 aatgccgcta ttatggtgaa gcgatggcag cggtgcaacc atacaatggt acgggagatg
     8341 atgcaattgc tcacgggtgg cgcggtttct tgacagctgt gcggaggcca gagtaggctc
     8401 aattcacaca agcttctcgc gccggtccaa catgatgaat ccaacctcac gccagtcgtt
     8461 ccgcgcacgc tttcttgcga tccttgttgt tgttttttgg ggaatagggg gagcagcgac
     8521 ggtttgggcc caagtgggga agccagaggg gctctactat aagtcctggg ccgtggtgat
     8581 cggagttgag aattacctcc tggctccaaa aattcccggt gtcatcgatg atgccaaggc
     8641 ggtggcacag accttccgaa aacttggatt tgatgaggtc gtggaattat acgacaaaga
     8701 cgccagttcc cgccgattgc agcagacctt gatggagttt ttgccgcgca aagtcggccg
     8761 tcaggaccgg ttggtgattt atttcgccgg ccatactgga atcacgcaag gtttgtcggg
     8821 gaaagacctt ggctatctcg tgccctggga tgcgcagatc gcgaacgtgt cgaaatccgt
     8881 cacgtttgaa cagctcaaag agttcagcca tcgcagcgcc tcgaaacata cgttgttact
     8941 cttcgatgcg gcggttcggg gatgggaagt cagcgcagcc cagaccctgt ctctcgaagg
     9001 ccggagcgcg ccggaggacg atacggaaaa acgcgccgtg caggtgttga cggccggcaa
     9061 caagggggaa tcactcgcgc ggaaacaaga ccacagcctg ttcgttcagg cgctggtcaa
     9121 agggttatcc ggtgaggcag atttggataa gaacggctgg ctgatggcct cggaggtagg
     9181 gcggttcgtc acgcagcaga tccaggatca gacaaaggga gcacaacacc caattttcga
     9241 gcaactggaa ggcgacggag ataccatcat tgtggaaggc cggaaggcgg cgttcaggct
     9301 tggcggagaa ccgcagtcgc cagccgagcg gcaacaggct gccaaaatgc actacgagca
     9361 ggcgtttgcc ctgttgcaaa ccggaaaggc ccccgatgaa gccctggagc gactgaacaa
     9421 ggctttggaa tatgatccca cattcggcga tgcgtatgtc ctcaagagct atgtccggtt
     9481 ggaggtcctg ccgaatctgg acgaggcact cgcagccgcg aatgctgcgg tgaaatacgc
     9541 gccgaagaat ccggattcgc agtataccct cggattgatt tatgagaagc gcggccaata
     9601 tgcggaagcc gaacgggcga tgcgcgaagc gctggtcgtg aatcccagct acctcgatgt
     9661 ctatttctca ctcgggacga tgtatgcgga cgaaatgaaa gatcagcaga aagctgtagc
     9721 tgcgtttgag cgatacctcg aattgggcgg taaccatacc cgtgctcgtg aagccgtcgc
     9781 gcagtcgcga gatgcaggaa caggtacccc tccgtcgaaa ccctaacagc ctgttgaaaa
     9841 agtctccctt ttcacccgcc cagccccggc gcgccgagac gcacccttca ccgaggcgcg
     9901 ttctcgcgtc gctcagaggc tcaacgtacg gagaagcccg cttgaggcaa agtaactatc
     9961 cgctcgccta tgatcgaagc gagcggccca agcgaagctt gatatgtacc tccttgcctc
    10021 atcgctcgct gcggccttgc gggacggcct ttttgaacag gctgcagact atgtgacggt
    10081 cgttcgaacc ttccggttcc ctgctttctg tcgactgaaa tggtttctca agagctgcta
    10141 gtccttgccg cctcccttga gataacccaa tccgatcttg agcgctcgtc tggtaatctc
    10201 tgcccagcga acctgcggat actctgcgag cacggcagcc gccttgggat cttggatgtc
    10261 gagattaaga attttaatgg tgccgtcgtc gatctgtacg tcagccacgg tgctcctccc
    10321 tcaatgaaaa gccgagcagt caaccatcga ctcgctgttg cgttgactga accaggggtg
    10381 catgttagca tgatttcgtt cgatttctcg aacgcttctg aagtcacgat gcctgcgcgc
    10441 ttggagaagt cgcggaaatt ttcttaagga ggacgtcgaa tgaaccagcc acaaggattg
    10501 atggcaaaag tgaccagtgg tgggattgcc ctgctgttgt tgatcgggct tgctgcatgc
    10561 ggcggccctc cgaagtgggt caagcagggg tccggtgcgt ttaacgacaa ggacagcaag
    10621 gccttttatg gcgtcggctc cgttgtcggt gtgagaaatg agccgctggc ttgggatacg
    10681 gcagaaaatc gtgcgcgagc tgaaattgcc aaaaccttcg aaacctacac ggggtatctg
    10741 atgagggatt acgcggcctc tacgacagcc ggtgacttca cccgcaatac ggaagaacag
    10801 aacgtcgagc gggcgatcaa gactgtgacg actgcgaccc tcagcggtgt ccgcccaatc
    10861 gaacgataca aagatgataa aaccggcacc tattatgtgt tgaccaagct gaaccttgaa
    10921 gagatgaaga acaatctgga gcaggccaaa gaactcaact cacaggttcg tgatttcgtc
    10981 cgtaagaacg ccgacaaact ctttgaccgg ctggagaagg aagaagaaaa gcgcgccaac
    11041 cggtaacggg cggagggcgt atcaaggagc atacgatgga aggttctgtg gtacgtgttc
    11101 ggtctggcgt cagtgttgct gtggtcattg gcgtcgcact cgcggcgttg agtggctgcg
    11161 cgcaggaaac caaggtgacg cgtgtggata ctggcgtggt cactgattta agcggtcggt
    11221 ggaatgatac cgattcacgg atggtcgcgg aggcgatggt aaaagaagcg ctggaatatc
    11281 cctggctggg caacttcagc cagtcgaagc atcgccaacc ggtggtggtc gtcggaaccg
    11341 tactcaacaa cagccatgag catatcaatg ttcagacgtt cgtgacggat ctggagcggg
    11401 aactgaccaa ctcccagaag gtcacgttcg tggctggaaa gggcgaacgg gaagaggttc
    11461 gggacgagcg caaggaacag gcgatgtacg cgagggaaga tacccagaaa gcccccggca
    11521 aggagatcgg tgccgattac atgatgaagg gtacgattgc gacgatcctg gacgaagcag
    11581 acgggaccaa agccatgttt taccaggtcg atctgcagat ggtcgacttg gagagcaatg
    11641 ccaaggtatg gttcggccag aagaagatca agaaagtcat cgagaaaaaa cggtcagttt
    11701 tttaggctcc acaagctccg ttgagctcat tcgtctcacg cctcaccgct catgcttcgg
    11761 cacggtgggg cgtttgtttt tccgtgcagt gcttttctgt tgcgctcctt ctgcccctcc
    11821 tgtcaggctg cggaccctca gtcagccgtt atctcctcgt cgacgatagt cttcgcgtgc
    11881 agaacttcca gcaggccgat gcgatcatcc agaaagcgga gaacgagtat ggaagcaaga
    11941 gtcgcgtgct gtacggcatg gaccgcggga tgacgctgca gctggccggc gagtaccaac
    12001 aaagcaatgc ggtcctggaa gaagccgaag acgaagttga tcgtctctat accagggtcg
    12061 tccgaactga aaccctggcg ttcatgacga acgacaacgc gctcccgtac gaaggcgacc
    12121 cctatgagca ggtcatgatc aatgtgctga aggcgctgaa ctatgcgtct ttgaaccaat
    12181 ggcaggacgc actggtggaa gcacgccgta tcgatcaccg cctgaacgtg ctctccgatc
    12241 gggtgcagga caaaggggcc tatcgcgatg atggattcgc acggtacctg agcggcatcc
    12301 tgtatgaaag cgcaggcgac gtgaacaatg ccttcatcgc ctatcggaag gcatatgaga
    12361 tctttgaatc aacaagtgcc tggtcacgaa gcagggtacc tgcgcaattg aaggctgatc
    12421 tcctgagaac tgccgacgcc ctccacttaa cttcggagct cacggaatat cagcgcgcgt
    12481 tcccggacac ttcctggcaa agcctggaag cgcaacaaca actcgctcaa gtcgttgtga
    12541 tcagctataa tgggcgcgcg ccacgcaaag aagaccagtt ccttgatctg ccgatcagtc
    12601 tcgatgcgct ccaattggtg ctgctcaatc ggggattcta ccaatcgaac cgccatcaga
    12661 atcgagcggc cgacagcatc ctttatgggc tcaacgggcg ggtggttcga gtggccgtgc
    12721 ccagactcgt ttcgcaaaaa acccaggttc cagtggatac ggtcaatttg atccctgaga
    12781 gtggagtgcc ggtaaccgtg cggacggagt tggtgcagag tgtgacggcc ttagccgata
    12841 aggcgttgtc ggagcgcatt actgcgatca ccgtgaaagc acttgcccgc gcagcgacga
    12901 aattcagcct ggccgagggg gcgactaggg gagcccagca agcagccggg agagacgcag
    12961 ccccctgggt cgggcttgtc gtcggcctac tgaccaaagg attggcggtc gcttcggaag
    13021 aatcggacaa gcgtagttgg cgaacactcc cagatgagat tcatctcgcg cgaacctggg
    13081 tgactccggg ccactataag gtgcagggtg cagcgttgac cggaacgaac aattcaggaa
    13141 atcttgctcc cccggaacga gtgctctcgc ttcatgccgg ggaaacagcg gtacttattc
    13201 aacgggtgat tccatgaagg tccaacgcat gacagggaca atcggagcgg gaatcattct
    13261 cggaaccgcc ctgttgtggg gatgtacatg gtttggcggc caggccaaac cggcctggat
    13321 cgatggcgtc agtcccgact atccgtccgc ccagtacttg accggagtgg gacaggggga
    13381 caatcgcgca gtggcagagg atcaggccta cgctgcgttg gcgcggatat tcaaggccga
    13441 agtatccgcc cagtcgaagg attgggaatc gtacctggtt gtggaggagc gaggacacag
    13501 taaggatgaa cggcggttga ctctggataa tttgacgcgc gtatcaaccg ataaagtgct
    13561 ggagaacgtg aaagtggttg atcggtggta cgacccgaag aaaggtctcc actacgcact
    13621 ggccggcatg catcggcctc aagcggagac ggcgttcatg gaacggatcg ccgaattgga
    13681 ccaatccatt cattcggatg ttgaggaggc gcaccattcg gcggataagc tggccaaagt
    13741 cagaggactc aggcgagccg ctcgcaatct cgtcctgcgc gagacctata acgcggatct
    13801 ccgtgtgatc aggccaagcg gtcaagggac agcggcggcc taccgggttc aagagctcac
    13861 ccgagagttg gaggattttc tttcctccaa tctcgtgctt gcggtgacgg tgacaggcga
    13921 tcaggctgag ccggtccagc gagccttgat agagggcttg atcaaagagg ggctgcaggt
    13981 ttccagccgg acaacgggaa ccgagcagca gactggtgga agcggcgtgt atccggctcc
    14041 cgagttactg gttcgcggaa tggtccgggt gtggcctatc gatgttcgcg accctcagtt
    14101 tacctacgtg cgctggtgca gcgatttcga ggtggtggat gtcacgagtc agcgggtggt
    14161 cggcgccctt tccaaggggg gaaaagaagg tcatttgacg gaacgagaag cgacggcaaa
    14221 agtcgtgagg gtaatgcagc aggacatgtc ggcggacgtg gccaaggcga ttgccgcgca
    14281 tgtcttcgga gagacggaac ttccggccca ggcgatccgg cccgcgggat gtcccaggga
    14341 aggatcggga tcgaagccgg ccacaataac ccactaacga tggaggggag agcgcgcgta
    14401 tgagcagacc gaatcaagct gggcgagcgc cgcgaagccg cggtcgagga ttgaccgcaa
    14461 cacaacaacg agtcccgagc cgattggcca aacggcggct gggcgagcgc ttgaagaacg
    14521 atacagcgct gttcaacaag ggcaacttgg ccgataagct tcgcaaagag ggttacgaag
    14581 aagtgccgtt ggatgagctg caggatcgac tctcaaaaat gcccgggccg ctggccgcgg
    14641 tcattttgaa agggaggatc tgatccatgc cgtactatta tttcgactcc acggccctcg
    14701 tgaaacgcta cagcatggag cgtggcaccc gtatcgtgaa caagctgatg gtcaagcgcg
    14761 gcaaagtcgc cattattccc acatggacgg tcacggaatt gtactcggcc ttctccaatc
    14821 gcgcccagca aggcgagctg acgcgcgacg attgctattc ggtcattcac aagttcgaac
    14881 gagaatctga ggaaggaatg tttcaattca tcgccccgac gatgcagacc tacctggcga
    14941 cgaaggagct ggtgatggaa tatcccttcc tccgaacatt gcaagtcatg catttggcgt
    15001 tggcctcgga actgaagccc ctgcgattga cggtcgtgag cgctgacata cagctgttgg
    15061 ccgcaagcaa gaccgccggg ctgcatatta tcaatccgga agaggattga tcgggggctc
    15121 gcgtctggcg agctgagtgt gccgatctgc ggctggccaa gcgttacggg cactggtagt
    15181 cggtgatcat ggaggcgacc acgatcaccc aatcgtctgc agtttcgcgc caccgcttcc
    15241 agcgttcggc gagtgggggc ggagacaaga tttcctgggc aaggtccgat aaggcgtgtt
    15301 gcacgttccc ctgagccagg gctagacttc cgcacacgcc tgaggaagcc ggaagttgtc
    15361 caccggccct ccggaacaga gccattgcgc ggtagccgtg ttcctgcgaa cggaacagcg
    15421 attcagctcc acggtacagc cgtatcgcga cattatcatc tttgttcaat aggccaagtc
    15481 gcttggccat gagggctgcg cgccccatgt tcatcgctgc gccttcagga tcgtcgttcc
    15541 cgatcgcttc ttcggccttg acccgcagcc ggtccatctc agccgcatcg cccatcacct
    15601 gagcaacact tcgttctatc gtgagaacac cgcatcccac acagagacag atgccgatca
    15661 ggaatggggc agtgctgacg gtgaagtata agcgcatggt ctcagttaat ctgatcgaat
    15721 ccgtcgagct gtttgatgct gtaattgacg gcttcccaaa gcttgccttc ttgggatcca
    15781 cgatctgttc ggacgtttcg cagcgagcgt tggagtgcca caagcggttc ataggctcgc
    15841 cggtccggga gttgtcctaa ggcccacacg acctcggtct tcagcacgag atcatcggtg
    15901 ttgagcgctt cgagcaaggg gggaatgatc gaggcatcgc cgtgcaacgc gccgactttc
    15961 cccaacgctt ttgccgcgac aaccttttga tcaggcgagc cggttttcag cattgaaatc
    16021 agcgaggcga tgctgtcctt gtggccgata ttacccaagg caatggcggc ggctttggag
    16081 acgtcaggat ttggatcttt gagcgcagcc tggagttggg gaacagcctg cgtagagaag
    16141 atttcgccca gcgattggat aatgcgcact cggcgggccg tgggtggatt gccctctgcc
    16201 aagactttca ggagacgtga gtcttgtccc caatccgccg tgacgaagac gggaaagtcg
    16261 tactcctcca gccgttgctt cagatgatca agcgcgtagg gtcccgcgtc agaagccttg
    16321 gcctgggcgg cagctgcgat ggcatcggca aatgctgttc gaacttcctt cttccactgg
    16381 gtactcttgc catccaataa ataggtgagc tggcaggccg gtcccttcca ggtccgcatc
    16441 ggcgaatccg ggagatcggc atcgcctcca gaagccatgg ttccttccca gaccttgcgc
    16501 tgctcgcatt tcacccgaaa gagtacatcg tacggctggg acgggtcggt aacgatggta
    16561 tagcccagct cgccgaatct ttttgcggtg aggtcgcgaa aaggtgaagc atccgtctga
    16621 ccttgatcgg tcaaggccag gacatccagc aggatccgat cgattttctg aagctgctcc
    16681 ttctgctgtc cggtcagcgc atcacgacgg gcccatgcgg tggagacgac cacaagtgaa
    16741 acgagcaggc agctgatggc gatccgtgcc agcgacatag acgcttcact gtaccgaacg
    16801 ggctgcaatc cttgcaagtt gcaaggattg tggagcgacc attataatcg ccggatgttg
    16861 cacgaccggc ggctcgcctg tattggcgcg ggaaatatgg cggaggcgat tgtcgccggc
    16921 ctgctcaagg gcaagcacgt atttccggca caattgatgg cgtcggacat ccgggccgat
    16981 cgccgcgccg acatcagccg aaaatatcag ttcgcgacag ccgagaagaa tgccgacgtc
    17041 gctgcgtggg ccgacgtgat cctcttggcc gtagagccgc aagtgctcga tgaggtgctt
    17101 caagagattg cacctgtcat aggagagggc acgcttgtgg tatcggtagc agccggctat
    17161 ccgatcgcca ggctgaagcg gtatctgccc gggacaaaac ggattgtgcg agcgatgccc
    17221 aatacaccct ccacgattcg agagggagtc accgcggtcg cgtatgctgc cggattggtc
    17281 gatgacgatg tcaggacggc gagagccctg tttgaaccga ttggaagagt ggtggaggtc
    17341 aacgaggggg cccttgatgc ggtgactggt ctgagcggca gcgggccggc ctacgtgtat
    17401 gtcatgatcg aagcgctggc cgatgggggt gtgaagatgg ggctcccgcg ggaaacggcg
    17461 cagctcctgg cggcgcaaac ggttgcgggg gcggcgcgaa tggtgctcga gtcgcgagaa
    17521 catccgggcg cgctgaaaga tcgagtggca tcgcctggag gcaccacgat tgcgggtcta
    17581 catgaattgg agcgaggctg cctgcgatcg acgttgatgt ccgcagtcga ggctgccacg
    17641 aagcgatcat cggaattggg gctagtcacc taagaaggag aggttaaccg tgcagtactg
    17701 ggtgaaagtg gtgttcgtag acaatcaaga actcatggtc aaagatgccg tcaggcacac
    17761 gatcagcgac gatatggaag tcttggaggt ggacacggca aaagaagtct tcatcatccc
    17821 gatgaaacag ctcaagtatc tcgcctgcga tgcgacggtg tttgccgcaa agaagcccgg
    17881 gtgagccccg ccgccgtctc tgttctcctg tcaagatttc cctcaggcca ccctgtccaa
    17941 gagtgctaag accgcttcgt tcaggccggc tgggccgatg cctggcgccc gtatcaatcc
    18001 cggaatctgg atgtcgggat cataggatcc gtcgggtttt tgcacgagaa tcgggtgatc
    18061 aaccattgcc agaagcggtg cgtcgttcac gctgtcccca atccccacgg tctcgatacg
    18121 atccggctgt tcgagcaacc gcagttggcg ttggtagcaa cgcaagagta gttccgctgc
    18181 ttcacctttg tcattgtccc ctgtcaggtg gaaaaaccgt cctcccttgg tccatcggag
    18241 ccctcgagag acgatttgcc agcagacttc ttcgatcaag tcttgagggc cttcgagcag
    18301 gaaaggctca tcgtactccc gcatcttggc aagtgttgca tcggtgcgtg agagaccggt
    18361 cagatgcatg acctcatcga cggacaggtc gccgaaccct cgaaggggag tttccacggc
    18421 gtcttcgatc tgtttgagca cgtcgcggag catgtggtac ggcaatccga gttcgataac
    18481 atcgtacgag gatttcgttc tgactcgttc gagaggaaag tcgaaaaggc cctggcgaac
    18541 aaagaccgcc gcgccgtttt ccacgatgaa cggatcgaga tggttcaacc gacgtcgaag
    18601 cggctccatt tccgctcgag ttttgctcga cactaggatc agaggaatgt tgcgcattcg
    18661 aagcgcgtca agggctggcg tggccatctc aaaagagtag gtctcgctgt cgaggaggga
    18721 accgtccagg tctgtgaaca cgacaaaacg agtcatcggt ctgcccgttg agcacgatgc
    18781 tgaatcgcgt gtcggaagct tcttccgtgc agggtgatcg gtctccatcg cggttaccag
    18841 tgatgcagat cgtcggtgac ggtcgatttg ttgcgacgaa tgacccgttg ctcaagaaat
    18901 tctttcgcga gatcggttgc gccgagaccg aaggcgatgg ccgtcgccag gacgacgcct
    18961 ccccatgtaa tgccgaatcc gacgacgacg atgtgttgag caatacccaa ctgttcgagc
    19021 gccatcgctc cggcgaggag ctggagtccc caccgtgaac agttggcgat cagtcgcgcc
    19081 ggcggaagtc cggcgttgac ggccgcgatc aagatggctt ggtaggcaaa gttcgaacag
    19141 acatagccgg ccaaaacgat gacgcctgcc gtgatgagat gaggtacgta cgcgagcaat
    19201 gattgggcaa attggttgat cggcgcgagg ttaagcgcgc caagggctgc gactgaggcg
    19261 actaacacca ccgcccaata gagcgttcgc ccgatgagcc gggagggatc cgtcttgatg
    19321 ccggctcgca ggagcgccgt cgtcgtgccc aagcggttgc tgaggtgatc aaatcctatg
    19381 acgcggagaa cacgctccac ggaaacgctc agcagccagg cggtcagaat cccgccgagg
    19441 agcagaatac ccatggcgaa cacattcggc aggatcgcga gtgcgtggcg accgagcagt
    19501 tgcaacggtt ccatgagggc ctgctcaaac aattcgttca tgactgcctc ctgcgtcggt
    19561 cacggttcgg gactgcaatc tcggcattgc cggctgccgg tccatttcct cccaacgcgc
    19621 gagtaagtag ggtttgtttt gctcgaatga ctggcagaga gtctcgatgg tctgttcggc
    19681 ttcggccgac gtcaggccct gcgtggcaag aacaaaggat gccgtccgcc ccagatacag
    19741 gggcgttaac gatttaagga ggtgctcacg cggcaggatt tgttcgtggt aggcgaccgc
    19801 ggcatcgtag acgatttgga cccagagctc atcggggaca cgggaacatg taggagcctg
    19861 tcgattgagg tcgtacagtt gctcaaacgt gagatcggcg agaatctgtt tccagattgg
    19921 ttcgagatcc cggagcccct gttgaaagtg agagaccatg ccctcgacat ccacgttgac
    19981 cggttccacc cccacctcgt agggaaatcc gaacagcgga accggttgcg agccttcgat
    20041 tccggaccag atagtggcat gctgttccat cgaggcgaag agtgaaccga cgacctgccg
    20101 cagcatagcg gagagatcgg cggcgggatc tttcgggtta tgaattttcg cccctaaaaa
    20161 actttggcag acgcgcgcgc cgctggcaat agcttcagtc gtcatccaga tgtcgatgcc
    20221 gaaacgagcg acctcggatt cccacacgtg ttgatcgaga taatgcttcg ccaatgcgcc
    20281 ggagaatccg aactcgccgc cgataggctg tcgaatacgt ttgccgtaga gtgatctcgt
    20341 gagcggataa gcaaggctgt tggtgatcgt gccgtcatat ttatgtctga gatagaacgg
    20401 ggcgacgtag tcgtagcctc cttcgcgtaa cggccgcagc aacatctcga tccattcggg
    20461 tgagatactt cgcagatctg cgtccacgac ggcgcaggcc tttgcattca gttgcctggc
    20521 gatctcaaag atcgtccgga acgcgctgcc ttttcccgga atcccatgat agggggtcac
    20581 gatgcgatgg agcacgcttt gccgatcggc aatgagcgta tggtgatgat cgatgaccgt
    20641 gcgagcgacg atcgcagcgg tctcgtcgga ggatccgccg tcggagttga cgaggacggc
    20701 cggttcgtcc ggaaagtact tggcaagacc ggcgctcacg gcgcggacga catgccccac
    20761 cgtgtcggca ttgttatagc tgggaattcc gacgaggata tcggctgaac cgatttcccg
    20821 cagacgggat tcagtcgcgg gagcgagtgg agtgtggttg gtgctcatgg cggcgtcccg
    20881 accggcgtgg aagtaggatc ccagacatgg tctgcctcga cggcttcggt catccgctcg
    20941 aaaatatcgg ggacggcatg cgcgacgcgg ctccagttgg aaatcatggg gaccccgagc
    21001 ggatcgtcca gaaacccgtc agtagctaac ttcatgcctt tcaagaaaac ctcgaccgcg
    21061 gtgcgttctt cgtgacgatc gaaagccagg ctgttaatgg cggcgtcgtt ctcgtaccgg
    21121 ctgatggctt cctgtgctgc ctgcagatat gtggcacgca gagtctttaa cacactgtcc
    21181 gataagacca ccccttcgct ggccaggttg cggaagaggg atttggtgat gtcgacacac
    21241 atcttcaaca gaccggcatc gggattttca ttcgacagcg cctgatgttt gtggtcgtac
    21301 gcgtccgcaa tgtcggcttg gcaaattcgg cgcaaggcac agtttcgata gacttcggcc
    21361 aggactccga tctcgagtcc ccaatctccg gggatacggt tggtccaggc gaggtcacgg
    21421 accatggcga attcaccggc aagggggtac cggaaactgt cgaggaacgt gaggagcgga
    21481 tgcgcgccga ccagcagctt aagactacgg atcaatggcg tgatgaagag gcgcgtcacc
    21541 cgtccatgaa gccggtcggt gacgcggctg taatagcctt tgcagaattc gtagcccagg
    21601 ttggggttcg cgatggggta acaaagtcgg gcaaggtact cccggttgta gctggtgatg
    21661 tcgcaatcgt gaagggcgat gacctggctc tggtttctgg ccagcacata tccgaaggcc
    21721 atccagcagc cgcgcccctt tccttggaga ccgatgtcga gattcttctc cacgagcaca
    21781 ttgaggatgg actggatgcg ggcaccgtcg ttccaaatca ggcggacgcg ttgagggagt
    21841 tgggagaagt actgcttggc taagcggaat tctagggctg aggctcgatc cagggagatc
    21901 acgatttcgt tgaggtagga cacccctttg agcgtttcca cgatccgttt cagggctgga
    21961 cggcttaact ccgcgtagag agacggcagg acgagcgcga tcggatggat attgccgtag
    22021 cgcgacagct ctcgttccaa ctgatcgacg ttggggtctc caagccgatg gagtaccgtc
    22081 accacgccat tctggtagaa gtccgacata ggcgtccccc cgaagatgca cgtggcacgg
    22141 aaaaattgct tccgctatgc agctggcgtg gtcctcgtcg ccaatgtggt aaggctagtc
    22201 acacgctatc cttgcacaaa aaccttaggc aaagtcaatg tgcacgccac atgccactga
    22261 aatttcccca aaaacgagcg tttgagagtg atggagaatc gctgagctga aggggtgatg
    22321 gccaaaacat tcactgccta aag
//
LOCUS       DATIFR010000147         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_672196, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000147 DATIFR010000000
VERSION     DATIFR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672196"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1440
                     /locus_tag="VLD60_08990"
     CDS             289..1440
                     /locus_tag="VLD60_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSB45135.1"
                     /translation="MAHHNTVFSQILKLVPRHEFESLAREHHQGRSLRKMTRWSQFVS
                     LGLAQLAGRVSLRDVVSNLKAQASKLYHLGCAEVNRSSLARVNEQQPASLYEALFGRL
                     LARCQGLSPKHRFRFKNKLYSLDASTIDLCLSVFPWAEFRTTKGAIKLHVGLDHDGYL
                     PSFLTITEGRVHEVNPARPLTLPKGSLVVFDRGYTDYAWYNHLNHQGVYFVTRQRKNA
                     CYRVIERHPLPTGSSVTSDQVIQLSGAKAGRCRGPLRRIGYRDPETGKHYVFLTNNFR
                     LSSKTIAAIYKERWQIELFFKWIKQNLKIKTFLGTSRNAVLTQIWVAMCMYLLIAYLK
                     FMSQLGLGMQQILRLLQLNLFERRELMALLRGDPPQPITSPLQARLQFA"
BASE COUNT          327 a          475 c          407 g          317 t
ORIGIN      
        1 ggaggagcca ggcgggactc gccgcaaatc cttccctgga ggctcgccgg ccgcgtccct
       61 gcggccgacg gtcccgcccg tctcctcctc ctgtcctatg aagaagatga caaaaccaac
      121 gactggctgt gtctcggtcg agggcggatt cacgccttca attaggggaa cgctgattta
      181 ttcgtcattc actgctgtcc cataaacgag gaacaaagaa ggcctgaacg atccgagctg
      241 ttacaatggt tgtgctcaca aacactgtaa tagaaggaga ttcaggcctt ggctcatcat
      301 aacactgtgt tctcgcaaat actcaaactg gttccgagac atgaattcga gtccctggct
      361 cgggaacatc atcagggccg aagcttgcgc aagatgaccc gctggtcgca gtttgtcagt
      421 ctgggtctgg ctcagctcgc cggccgggtg agcctgcgag atgtggtcag caacctcaag
      481 gcacaggcca gcaagctcta tcatctgggg tgcgccgagg tgaaccgctc ctccctggct
      541 cgcgtcaacg aacagcagcc cgcgagtctc tatgaagccc tgttcggcag actactggcg
      601 cgctgccagg gactgagccc aaagcaccgc ttccgtttca agaacaaact ctattcgctc
      661 gatgcctcga ccatcgactt atgcctatcg gtcttcccct gggcggagtt ccgcaccacc
      721 aagggcgcca tcaagttgca cgtcgggctg gaccacgacg gctacctgcc gagcttcctg
      781 acgatcaccg aaggccgggt gcacgaggtc aatccggccc gccccctgac gctgcccaag
      841 ggcagtctcg tcgtcttcga tcggggctat accgattatg cgtggtataa ccacctgaac
      901 catcaaggtg tttacttcgt tacccggcag cgcaagaacg cctgctaccg ggtgatcgag
      961 cggcatccgc tccccacggg atcgtcagtg acctcggatc aggtcatcca actgagcggg
     1021 gccaaggccg ggcggtgccg cggcccgctg cgccgcatcg gctaccgcga tccggaaacg
     1081 ggcaagcact acgtcttcct gaccaataac ttccgtctgt cgtccaagac catcgccgcg
     1141 atctacaagg aacgctggca gatcgagttg ttcttcaaat ggatcaagca gaacctgaag
     1201 atcaaaacct tcctcggcac ctcgcgtaac gccgtactga cgcagatctg ggtggccatg
     1261 tgcatgtacc tgttgatcgc ctacctcaag ttcatgagcc aactcggact cggaatgcaa
     1321 cagatcctgc gcctgctgca actcaacctc ttcgaacgcc gcgagctgat ggcgctgctg
     1381 cggggcgatc caccgcaacc cataacctcg ccacttcagg cacggttgca gttcgcgtaa
     1441 tgtttatggg acagcagtga gaattaatac ataccaatgt tctagctatt aattgttttc
     1501 tttccgaagc ttctctgcta tcgtca
//
LOCUS       DATIFR010000148         5996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2778984, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000148 DATIFR010000000
VERSION     DATIFR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2778984"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1787)
                     /locus_tag="VLD60_08995"
     CDS             complement(393..1787)
                     /locus_tag="VLD60_08995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB45136.1"
                     /translation="MAGSHSNLPDALPVPDPVIAARLEAIKQLAGGFSERVAVMDRDF
                     NVIYANKSAWSQEDRANQPLHPAKCYDAFAHQGDPCEACPAVKVFEAPDVQCVSCSNG
                     GDGTACGMQQAFPLLAGNGQVALMLVLFEPTAKPNLRPEAHVPEEIAPPMVREHLGDL
                     IGRSSAMQQLFDMISMVADSSATVLIQGESGTGKELVAKTIHELSYRRAKPFVVVDCG
                     SLPEALLESELFGHVKGAFTGAVTNKRGLFEEADGGTIFLDEIANTTPTFQAKLLRVL
                     QEGEVKRVGSNQRVKIDVRVISATNKDLTEMVKAKSFRQDLFYRLAVLPLNLPPLRDR
                     KEDIPLLVEHFVASSCKRHRQPVRHVSPDVMHALTEGEWPGNIRELQHYVERAVVTTA
                     GPDLTCKDIVAMGSHVEPKDLRAVARDAVTHAERARILEALKKTGGNRARAAKVLKIS
                     RASLYNKLREYRLE"
     gene            complement(1845..2243)
                     /locus_tag="VLD60_09000"
     CDS             complement(1845..2243)
                     /locus_tag="VLD60_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45137.1"
                     /translation="MVTKRTAVVLVVEDDREMRSLLCDEFWSAGYQLREAKDGDEAFL
                     AVLQAVPDLILTDLRMPAGGADYISRLRTVAPSCPIVVMTAFGDSALKTSVLRAGANA
                     YFDKPVRIADLKACVERLLAERLGVRPGST"
     gene            complement(2502..4403)
                     /locus_tag="VLD60_09005"
     CDS             complement(2502..4403)
                     /locus_tag="VLD60_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB45138.1"
                     /translation="MHPPVNKVILFESGAVLFLTIGIFVLDILTPRGWAIWLLYLLPL
                     ALSLQSPRERDPYYFAAIVMPLTIAGGYLSPPGGIDLAANILNRSMGITVMWAFTWLM
                     VRQKKARVLLAGAETARSRAETQREAAVAARELAEASAVGAIHRESQTARELLRSNLR
                     LESILQSAMDAIVTVDSDQKIVLFNQAAERMFQCPVTEAIGQTLDRFIPARYRETHHR
                     HVESFGRSGVTSRKMGELGTVTGLRADGEEFPVEAAISHNTVDGKRFFTVILRDITER
                     KQAERLLRQSEERYRRLISVSPLAVIVNRGNRVIFINDAGVRLFGAVKAEEILGKSPV
                     DLFHPESRALVGKRISQLLEGSPNVPVVEEKIVTLDGTSIDVEVNAARFMDEEGPAIV
                     AMIWDVSERKRLQEQLRRTERIAELGTLASGMAHEIGTPMNVILGRAEYLMDRVKDEP
                     VKRGLQTIVVQVERITRVMNQLLAFARRKSPDRGPVALKAIIENSVEMFQERLARNRV
                     LVELKAEEPCPNVLADIDQMSQVLINLIMNAVHAMPDGGTLRIGLTQENDMVKLTVAD
                     TGHGIPQEALKKIFEPFFTTKEFGKGTGLGLTVVKGIIEEHQGSIAVESLPGGGTTFT
                     ILLPAVPAS"
     gene            complement(4437..5813)
                     /locus_tag="VLD60_09010"
     CDS             complement(4437..5813)
                     /locus_tag="VLD60_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB45139.1"
                     /translation="MIEECGAILVVDDDADMREMVHDMLKDRGHQVTTAGNGQEALKI
                     LGEEEYAVVLSDLRMKGMQGLELLAEIKRLHPEINVILMTAFGSVETAVEAMKHGASD
                     YLTKPVRKDELVRVIERVVREASLRREVNRLRKAVHKEYSFHQILGKSKAMQGVFDLI
                     KRVADSPTNVLITGESGTGKELVAKAIHFNSDRKDAPFIPVNCAAIPEQLLESELFGH
                     MRGSFTDAKFDKRGLFEEAQKGTLFLDEISELPLMLQAKLLRAIQEREIRRVGANKPV
                     PVDVRIIAATNLNLGEEVKAKRFRDDLYYRLNVIELKLPPLRDRREDIPLLVEAFLRK
                     CGESRGKDVKGVSEAALAMLMDYAWPGNVRELENVIERAVTLSRGEKVSPDDLPSAVQ
                     GARGDRRVLDEAAEKTLPLHEIEKEYIKKILEKMGGNKYQAAHVLGIDRKTLYRKLGE
                     IEEGKAHE"
BASE COUNT         1218 a         1821 c         1609 g         1348 t
ORIGIN      
        1 cagaagagaa ccggcctccg actgaccccc ggccctgatt ctggagggat tagagctggt
       61 gaagggttcc ttcttgcgaa ggagtgggtc attcggaggc cggtcctaat cacccaggcc
      121 ttcaccagcg gtgcttcacc agcgcaacag ctgtgccggg attgggatcg acccacgaaa
      181 tgtgaaaatg attcgttggg aacacgagtt acgtaagaaa ggcagctggg gatcgtacga
      241 tgcgccgtga tgcaaatttc tactgagtta gacagttgag agtaggccaa aaaccgatct
      301 gtgatctttc aatgacttgt gctgtctaac aggttggaca gtttgcaggg tggaattgag
      361 gcggagaggg gcaaacagga gcgagggagg ggttattcga gtcgatattc ccggagcttg
      421 ttgtagagac tggcgcggct gatcttgagc actttggccg ccctcgcgcg gtttccgccg
      481 gtcttcttca atgcttcaag aatgcgggcg cgttcagcat gtgtgacggc gtctcgagcg
      541 accgcccgga gatctttcgg ttcgacgtgc gatcccatgg ccacaatatc tttgcacgtc
      601 aggtctggtc ccgctgttgt gacaaccgcc cgctcaacgt agtgctgcaa ctcacggatg
      661 ttaccaggcc attcaccttc tgtcagcgcg tgcataacgt ccggggacac atgacgcacc
      721 ggctggcgat gccgcttgca cgacgacgcg acgaaatgct cgaccaaaag gggaatgtct
      781 tctttccggt cacgcagcgg tggcagattg agcggcagta ccgccaaccg gtagaaaagg
      841 tcctgccgaa aggatttcgc cttgaccatc tccgtcaggt ctttgtttgt cgcggaaatc
      901 acacgcacat cgatcttcac gcgctggttg cttccgaccc gtttcacctc tccttcctgc
      961 aacactcgga gcagtttggc ctgaaacgtc ggcgtcgtat tggcaatctc atccagaaag
     1021 atcgtccccc cgtcagcttc ttcgaacagg ccccgtttgt tcgtcaccgc cccggtgaac
     1081 gcccccttca cgtgaccgaa caactcgctc tccagcagcg cttcggggag cgatccacag
     1141 tcgaccacga caaagggctt tgctcgacga tagctcagtt cgtggatcgt ctttgcgacg
     1201 agttccttgc cggttccgct ctctccctgg atcagcaccg tggccgagct gtcggcgacc
     1261 atgctgatca tgtcgaacag ctgctgcatg gccgagctcc gcccgatgag atcgcccaaa
     1321 tgttcccgca ccattggagg cgcgatttct tccggcacat gggcttccgg tcgaagattc
     1381 ggttttgccg tcggctcgaa caacacaagc atcagggcaa cctgtccatt gcctgcgagc
     1441 aagggaaagg cctgttgcat cccgcatgcg gtaccgtccc ctccattcga gcaggatacg
     1501 cattggacat ctggtgcctc gaatactttg acggctggac aggcctcgca ggggtcgccc
     1561 tgatgagcaa aggcgtcata acactttgcg gggtgcaatg gctggttcgc tcggtcctct
     1621 tgggaccaag cagacttgtt ggcataaatg acgttgaagt cgcggtccat gaccgcgact
     1681 cgctcggaga acccgccggc cagctgcttg atcgcttcca gtcgcgccgc gatcactgga
     1741 tcgggaactg gtagggcgtc aggaagatta gagtgagatc cggccatctc gtatcgtcct
     1801 tattccgttg ggctgaagaa tcagaatcga ttcacacgat tgtgtcacgt actccctggc
     1861 cgcaccccca ggcgttcagc cagcaatcgt tccacgcatg ccttgaggtc ggcaatgcga
     1921 accggtttgt cgaaataggc attggcgccc gctcgcagca cgctggtctt caacgctgaa
     1981 tcgccgaacg ccgtcatcac gacgatggga caactgggtg ccaccgtccg caggcgactg
     2041 atgtaatccg ctccgccggc cggcatacgg agatccgtca gaatcaggtc tgggacggcc
     2101 tgcaagaccg ccagaaatgc ctcgtctcca tccttcgctt cacggagctg ataccctgcg
     2161 ctccaaaact catcacagag cagactgcgc atctcccggt catcctcgac caccagcacc
     2221 acagcggttc ttttcgttac cacagtcatc attcctttag tccaatccca atccttctaa
     2281 tgttcgtgga tggcaaacgc cgtgcctatg cggaagcagg cccgattgac acttttcttg
     2341 ggaaatccgg aatcgaagac ggcttctggg tcttgcgctg gtgagaaaaa cctcattcga
     2401 cagggctatg actggcgcaa gatgctacag aaccaggctt gggctggggt cgggattagt
     2461 caccgggcat ggagactaca cctcgtccct cggtcgtacg ctcaactagc gggaaccgct
     2521 ggcaggagaa ttgtgaacgt tgttcctccc cctggtagac tctcgaccgc aatcgaaccc
     2581 tggtgctctt cgatgatgcc tttcaccacg gtaaggccca atccggtccc cttgccgaat
     2641 tccttcgtgg tgaaaaacgg ttcgaagatc ttcttgagtg cctcttgcgg aatcccgtga
     2701 ccggtatccg ccaccgtgag ttttaccatg tcgttttcct gcgtcagacc gatccgcaag
     2761 gttcctccat ccggcatcgc atggacagca ttcatgatca aattgatcag gacctggctc
     2821 atctgatcga tatcggccaa cacgttcgga cagggctctt cggctttcaa ctctacgaga
     2881 acacggtttc tggccagtcg ctcctgaaac atctcgacgc tgttttcgat gatggctttc
     2941 aaggcaacgg gtccgcggtc aggagatttg cgccgcgcaa aggccaggag ttgattcatg
     3001 actcttgtga tccgctcgac ctgcaccaca atggtctgca aacccctctt gaccggttcg
     3061 tccttcactc gatccatcag atattcggcc cgaccgagga tgacgttcat tggcgtaccg
     3121 atttcatgcg ccatcccgga ggccagtgtc ccgagttcgg caatccgttc agttcggcgc
     3181 agctgctcct gtaagcgttt ccgttcactg acgtcccaga tcatggccac gatggcgggc
     3241 ccctcttcat ccatgaaccg ggcggcgttg acctcaacgt cgatggacgt tccatccaac
     3301 gtgacgatct tctcttccac gaccggtacg ttcgggctgc cctcgagcag ttggctgata
     3361 cgtttgccaa ccagcgcacg actctcgggg tggaacaaat caacgggcga tttcccaagt
     3421 atttcttctg ctttcacggc cccgaagagt ctcacgcccg cgtcgttgat aaagatcacg
     3481 cggttcccgc gattgacaat gaccgcgagg ggggacacag aaatgagacg ccgataccgc
     3541 tcttcactct ggcgcaataa acgctcagcc tgcttgcgct ccgtgatgtc ccgaagaatc
     3601 actgtgaaga accgcttgcc atcgacagta ttatgcgaaa tggccgcctc gaccggaaat
     3661 tcctccccat cggcgcggag ccccgtcact gttccaagct ctcccatctt gcggctcgtc
     3721 acaccggacc ggccgaagga ttcgacatgc cggtgatgcg tctcgcgata ccgagcggga
     3781 atgaaccggt ccagcgtctg tccgatggcc tctgtgacgg gacactgaaa catccgctcg
     3841 gcagcttggt taaacaacac gattttttga tcgctgtcga ccgtaacaat ggcatccatc
     3901 gcggattgca gaatgctttc cagccgcagg ttgctcctga gaagctctcg cgcggtctgc
     3961 gactcgcggt gaatcgcccc tactgcactg gcttcggcca gctcacgggc cgcgacggca
     4021 gcttcacgtt gggtttccgc tcgtgatcgt gcggtttccg cccccgcgag tagaactcgt
     4081 gcctttttct ggcggaccat cagccaggtg aaggcccaca tcacagtgat gcccatactt
     4141 cgattcagga tattggctgc gaggtcgatt cctcctggag gagacaggta cccaccggcg
     4201 atcgtcagtg gcatcacgat tgccgcgaag taatagggat cccgttcacg gggagattgg
     4261 agcgagaggg ccagaggcag aaggtagagt aaccatatcg cccagccgcg cggagtgagg
     4321 atgtccaaga caaaaatgcc gatggtcagg aacaggaccg cgccagattc aaagaggatg
     4381 accttattga ccggtgggtg catgggaact caggtcgatt gaaaggaaga actagtctac
     4441 tcgtgagcct tcccctcttc aatctctccg agcttgcgat agagggtctt gcgatcgatc
     4501 cctaacacat gggcggcttg atacttattc ccgcccattt tctcgaggat cttcttgata
     4561 tattctttct caatctcgtg caatggcaat gtcttttccg cggcctcgtc cagtacgcgc
     4621 cggtctcccc gcgcaccctg cacagccgac ggcagatcgt ccggggaaac cttctcaccc
     4681 cggctgagcg tcacggcccg ctcgatcacg ttttccagct cccggacgtt cccaggccag
     4741 gcatagtcca tcagcatggc caaggccgct tcgctgactc ccttcacgtc ttttccacgg
     4801 gattcgccgc acttccggag aaacgcctcc accagcaagg gaatgtcttc gcgacggtct
     4861 cgcagcggag ggagcttcaa ctcaatgaca ttcagccgat agtagaggtc gtccctgaac
     4921 cgtttggctt tgacctcttc accaagattg aggttggtcg cggcgataat acggacatcg
     4981 acgggaaccg gtttgttcgc gccgacccgc cggatttccc gctcttgaat ggcccgcagc
     5041 agtttcgctt gcagcatgag cggcagctcg ctgatttcgt cgaggaagag cgtgcctttc
     5101 tgggcttctt cgaacagccc ccgcttgtcg aacttggcat cagtgaacga tccgcgcata
     5161 tggccgaaga gttcgctctc caataactgc tcgggaatgg ccgcacagtt gaccgggata
     5221 aacggcgcat ccttgcggtc gctgttgaag tgaatggctt tcgccaccag ttcctttccg
     5281 gttccactct ctccggtgat caggacgttg gtggggctgt cggccacgcg cttgatcaga
     5341 tcgaagaccc cctgcatcgc cttactcttc cccagaatct gatggaaact gtattccttg
     5401 tggactgcct tccgcagacg attgacttcg cgacgaagcg atgcttcacg aacgaccctt
     5461 tcgatgacgc ggaccagttc atcctttctc accggcttgg tgaggtaatc actggcgcca
     5521 tgtttcatcg cttcgacagc cgtctcgacc gatccgaacg ccgtcatgag aatcacgttg
     5581 atctcgggat ggagacgctt gatctccgcg agcaactcca gtccctgcat gcccttcatg
     5641 cgcagatcgc tcagcactac cgcgtattct tcttctccaa ggatcttcaa agcctcctgc
     5701 ccgttgccgg ccgttgtcac ttgatgccct cggtccttga gcatgtcgtg aaccatttcg
     5761 cgcatgtctg catcgtcatc gacaacgaga attgcaccgc attcctcaat cattcccatc
     5821 tcctccgcac tcataatcga cgcagcctag cccccgaggg cgaactggtc aagctccact
     5881 tgccccatta tgcgccagac gcaggtaagc cttagcccaa aaatccccaa aattcgtcga
     5941 ctgccggtca aacccagctc aaattctagc caactgcaaa ggagaggcca attcta
//
LOCUS       DATIFR010000149        15102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_863292, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000149 DATIFR010000000
VERSION     DATIFR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863292"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..846)
                     /gene="nadD"
                     /locus_tag="VLD60_09015"
     CDS             complement(127..846)
                     /gene="nadD"
                     /locus_tag="VLD60_09015"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSB45140.1"
                     /translation="MMSADSKPRLSALGTQHSGTLRLGLFGGSFNPVHNGHLTIARQA
                     CDALRLDRVLFIPTSDPPHKPHQSLAPAKDRYEMVRLAIASDPLFSISDVEFRRPGKS
                     YTIDTIRLLQQEYGTDAALFFLIGLDAFLDFPSWREPGALLNLCSFVVISRPGLAFSS
                     LSGLTILPPLPAQSLADLDAARQTTLEIAVGEHHLILLRLPPCEVSASDIRTRVKQAL
                     PVANLLPPLVESYILHHHLYE"
     gene            complement(877..1998)
                     /gene="proB"
                     /locus_tag="VLD60_09020"
     CDS             complement(877..1998)
                     /gene="proB"
                     /locus_tag="VLD60_09020"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSB45141.1"
                     /translation="MRDEILKQAKRIVVKIGSSLVSSREVGLQPERIDRLADEIAALR
                     SSDRELLIVSSGAIVSGIKKLALKGYPKSLPVKQAAAAVGQSRLMWAYEKSFERLGLR
                     VAQILLTHQDLADRRRFLNARHTLAALIDFGVIPIINENDTVAVEEIRVGDNDSLAAE
                     VAHLVDADLLVILSDVDGLFTEDPRKNPSAQLIPLIGEINDDIERRAGVSTTFEGTGG
                     MATKVRAAKKVGEYGVATLILNGQRAGLLPTVLSGGDGGSLFLAKERRLTSRKHWIGY
                     TLRPRGTITLDQGAVEALAKRGKSLLASGILNVTGLFEPGDPVSCLDQDGKEFAKGLV
                     NFSSDILGKIKGLRTAEIQQQLGPQEYEEVIHRDNLVIL"
     gene            complement(1980..3005)
                     /gene="obgE"
                     /locus_tag="VLD60_09025"
     CDS             complement(1980..3005)
                     /gene="obgE"
                     /locus_tag="VLD60_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSB45142.1"
                     /translation="MFVDEVRITVKAGRGGDGSCSFRREMFVPRGGPDGGDGGNGGDV
                     IFTASHRLTTLLDLRYQKHYEAEDGKPGGGSNCSGRRGKPVVVMLPVGTVVYDDDTGE
                     ILADLVTDGETSTIAHGGKGGRGNSHFATSTNRVPTHFEVGTEGEERQLRLELKLLAD
                     VGLVGFPNAGKSTLIAAISAARPKIANYPFTTLIPNLGVVRYGEDRGFVVADIPGLIE
                     GAHEGKGLGIQFLRHIERTSLLLHLIDVSDWATEDPVASFEVMRNELASYDPALAKRP
                     FAIVATKLDAAGDGTRLKTLQSYCKRHRYRCLPISAATREGLPELIAFVGQQVEQLRT
                     AACETKS"
     gene            complement(3130..3393)
                     /gene="rpmA"
                     /locus_tag="VLD60_09030"
     CDS             complement(3130..3393)
                     /gene="rpmA"
                     /locus_tag="VLD60_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSB45143.1"
                     /translation="MATNKGGGSSRNGRDSNPQYLGVKAYGGETVKAGAIIVRQRGTK
                     FFPGFNVGLGKDHTLFARVTGIVKFEGGRARQKVSVYPAPVAS"
     gene            complement(3406..3720)
                     /gene="rplU"
                     /locus_tag="VLD60_09035"
     CDS             complement(3406..3720)
                     /gene="rplU"
                     /locus_tag="VLD60_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSB45144.1"
                     /translation="MYAIIETGGKQYRVENGTLVQVESLPGEVGGTVELSQVRLVHSD
                     QGVLVGQPLVKGAKVKAEIVDQGRTRSIMVFKKQRRKNYRRTKGHRQGFTKLRITGIE
                     TA"
     gene            3936..4307
                     /locus_tag="VLD60_09040"
     CDS             3936..4307
                     /locus_tag="VLD60_09040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB45145.1"
                     /translation="MKASSRAQVKKTTSRFIMRPYRRIQTWYLSYYMSGDLVGKGVVT
                     NLSCSGMRVLGDHAMRLGTDLTVRLTLEEDKPPLEIERVTVQWVKEAEFGLKIVRISP
                     SAATRITHLLAFQARAHRGES"
     gene            4398..5309
                     /locus_tag="VLD60_09045"
     CDS             4398..5309
                     /locus_tag="VLD60_09045"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSB45146.1"
                     /translation="MEPQGKIWMDGSFVDWADANVHVLTHSLHYGLAAFEGIRCYKGK
                     SGAAIFRLQEHVDRLFDSAHIGMMTIPYEKKQVAEAIVETVRVNKLDACYIRPLVYIG
                     YGAMGVYPGDNPIKLAIAAWKWGAYLGDDAIANGMRARVSSFTRHHVNVSMTRGKISG
                     YYVNSILAKREAKADGYDEAILLDPEGYVSEGTGENIFIVRHGRIKTTPLTSILEGIT
                     RNSVMDLAREQGIPVMEERFTRDEMYIADEVFMTGTAAELTPVREIDNRRIGTGKPGP
                     ITQALQKRFFAIVRGEDATHESWLTRV"
     gene            complement(5332..5682)
                     /gene="secG"
                     /locus_tag="VLD60_09050"
     CDS             complement(5332..5682)
                     /gene="secG"
                     /locus_tag="VLD60_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSB45147.1"
                     /translation="MHTLVIIIHIVACFLMIGAILLQSGKGAEIGASFGGSSQTVFGS
                     RGPANFLSKFTVVVAIVFMVTSFSLAILAKQKTFSSTVIDLKKKETSQPAPAAPADKP
                     PAESHSSGDSSTAH"
     gene            complement(5693..6466)
                     /gene="tpiA"
                     /locus_tag="VLD60_09055"
     CDS             complement(5693..6466)
                     /gene="tpiA"
                     /locus_tag="VLD60_09055"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSB45148.1"
                     /translation="MRIPLIVGNWKMNKTASEAAGFVRELAAKLASSSRAEIVIAPPF
                     TALESARTALGPSSRIKLAAQNLHGESQGAFTGEVSGPMLRDLGCTYVIVGHSERRTL
                     FGERDGDIQKKVRAALVHGLRPILCVGESLAEREADRTDAVVTAQLTAALAGLTAQDL
                     SVVTIAYEPVWAIGTGHAATTAQAVAVHRSIRACMANASNQGTAAEMRILYGGSVTPQ
                     NAESLLCVDGIDGALVGGACLKSDSFATIVAAAENKSIH"
     gene            complement(6510..7703)
                     /locus_tag="VLD60_09060"
     CDS             complement(6510..7703)
                     /locus_tag="VLD60_09060"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSB45149.1"
                     /translation="MHKQTIDDLSLSGKRVIIRADFNVPLDDSLQITDDTRIRSTLPT
                     INRVVDDGGKVILCSHLGRPKGKFDPKYSLAPIAKRLGRLLGKDVIFAPDCIGPAIEG
                     LVAKMKPGDVMLLENLRFHLGEEQNDEGFSKSLAALGDIYINDAFGAAHRAHASTVGI
                     TKFIPAAAAGFLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKRVDKVI
                     IGGGMAFTFLKAKGLEIGNSLVENDMLDFAKGIEDHALSRGVKFYLPVDCVVAASREP
                     GAETKIVPVQEIPKGWYALDIGPASVKLFTEAVQNAKTILWNGPMGVFEIDAYARGTL
                     SIAHAVANAYALTIVGGGETAMAVHRAGESESISFISTGGGAALELLEGKQLPGLTAL
                     PERKD"
     gene            complement(7773..8786)
                     /gene="gap"
                     /locus_tag="VLD60_09065"
     CDS             complement(7773..8786)
                     /gene="gap"
                     /locus_tag="VLD60_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSB45150.1"
                     /translation="MAIRVGINGFGRIGRNVLRASLGDPELQFVAINDLTDAKTLAYL
                     LKYDSVHGTLKGSVEAKDDQILVDGKPIKVLAVKDPKELPWKDLNVDIVIESTGRFTD
                     REGAGKHLSAGAKYVIISAPAKDPDATVVLGVNDNTLDPKIHHIVSNASCTTNCLAPV
                     AKVLMESFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VIPQLKGKMDGLAIRVPTPNVSLVDLTVETEKDCDVAGVNEAFKKAANGPLKNILQYS
                     EDPIVSIDQKGNPHSATLDAPLTTVIDKRLVKVTAWYDNEWGYSCRVRDLIKVIAGKA
                     KDR"
     gene            8928..11426
                     /locus_tag="VLD60_09070"
     CDS             8928..11426
                     /locus_tag="VLD60_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HSB45151.1"
                     /translation="MAMNLRNGLRGGILCLASPMAGSLESSPHSVTGLGHEVIVKLFE
                     QTTQVLEWGKLLEVLASHGRSTMGAARCRAAVLPTDLVEAQRRQEETVEVARLQETAD
                     PPPSLSFPDIREPLARAAKGAALDAHELRDHALVLALWDEVQRYLSRHAQEAPTVAAA
                     AEVLRSGRELKELSRALDGAIHPDGSIKESATPELRRFTHHANDLKQQIRHQLDQILQ
                     SRRFAELLQEQYFAQREGRYVVPIKTEMQGRVPGIVHDVSSSGATVFVEPRELVELNN
                     AIKVADLEIDREIRRILRELSSLVAEKSDLILAGLDVLAELDCIAARAAFGRQLRAHP
                     VGLNDHGRVRLKQARHPLLVLSKAQVVANDILLEDHVQVLVISGPNTGGKTVTLKIVG
                     LFALMVRAGLLLPCEPESEMAIFQDIYADIGDAQDLARDLSSFSAHMTQMIHLLKDAH
                     RARQALVLLDEPVTSTDPIEGAALAEALLCRLASLGMKVIATTHYRSLKALAQTTAGF
                     TNASVEFNVATLSPTYCLFMGVPGGSSAIEIAGRLGMDRGLLDEARQKLEQDDRVLER
                     MLDDLHGKQRQLTEDLARAVAARAEAEEAAQLAKDQLARLEATEREERKGIKKKLQEQ
                     FSRARAEVQATVEEVKREQKLIKAKEAKQRLVELEAQTRAEFTPSGTPIPLEQLGAGD
                     QVEIGRLGMTGTLLEAPQGKKRVRVKVGEGELLATVANLIGLARGTTSLPAAVQSAPS
                     KPLGSTRPYHAEEQTVVDVRGKAADDALDEVVAALDRATLEATPALRIIHGHGTGKLK
                     AALREYLKSSPYVASARPGDRAEGGDGVTIVTVR"
     gene            complement(11437..12795)
                     /locus_tag="VLD60_09075"
     CDS             complement(11437..12795)
                     /locus_tag="VLD60_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB45152.1"
                     /translation="MHPPIIALISADLSTGPLLEQAFSNPNTTLVTAATTDEGLRLIR
                     TRHDLSLIVCDNGAGQIARELVLAQARQKARPVPIIVLGKEDSAKAAVEAMHQGATNY
                     LPKPLMVEVLRRALQQAEAYYATSEPPVREHPQGFDRIVSVSPQMRLVKHLAEEVALT
                     DATVLITGESGTGKELFSQAIHATSPRAKAPFIALNCAGIPEHLLESELFGYQRGAFT
                     DATQNKPGRFQLAEGGTLFLDEIGEMSLTAQAKLLRVLEDHVVDPLGGTQSQKVNIRV
                     IAATNEDLPANIKAGRFRLDLFYRLNVYQLRIPPLRERLDDIEPLVMSFLDFARRAHG
                     CRISGITPAALTLLQRYDWPGNVRELHNVVEGLTITCKDETIRPEHLATSFRAGQQSA
                     LSNSAEKPSLLSFGLSFQDMEKTLLEEALRKAAGNVSEASRLLKITRNTLRYRMAKYH
                     IQ"
     gene            complement(12925..13902)
                     /gene="queG"
                     /locus_tag="VLD60_09080"
     CDS             complement(12925..13902)
                     /gene="queG"
                     /locus_tag="VLD60_09080"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HSB45153.1"
                     /translation="MSLAEAIKREARALGFSAVGICRITDCTPPSTDKSDTDSLPRRL
                     YLRLQEWLRRGYHGTMAWIAREPSRRTDPRLVLPGCRSLISVGMNYDTGHRPTEGPGY
                     GRVARYAWGKDYHQVLGDRLKQLEARILDLAPGNLTRSYVDTGPIMEKAWAQEAGLGW
                     IGKHSNLVSSEWGSWLLLGEILTTLDLPPDQPGTDLCGSCSLCIQACPSGAIVEPYVV
                     DANRCISYLTIEFRGTREEIPDELPSQMGHHIFGCDDCLDICPFNLRADHTSEPAFAP
                     SGLTQAPRLDELLNMDERSFHTTFKDSPIRRAKYTGFQRNLRIARQNQS"
     gene            complement(13981..14355)
                     /locus_tag="VLD60_09085"
     CDS             complement(13981..14355)
                     /locus_tag="VLD60_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45154.1"
                     /translation="MTMLILRAVVCLLPLIGLMMYQPSEAGAQAPSDFPATTEQSCSF
                     GMAKGEPSQHCQVPIPAGCLVANTPGTNKPWTTISKGGKTFCRFDEKATDWKTKITGT
                     CGRCSTGHCSVQFIVRFDCNPH"
     gene            14449..>15102
                     /locus_tag="VLD60_09090"
     CDS             14449..>15102
                     /locus_tag="VLD60_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HSB45155.1"
                     /translation="MLATRIRQKEGTFYFVAYNAVDLLEKVRFTSRYFFEGEEIAQGK
                     ISEHDEVAQFIAGIERSEKGFQRLLNRQKIKQIVNFYETVVAQPMIPGTVLIFTDETL
                     RFQRAGESESIGHLSEPKGKYLVIDGQHRLAGLHFFRAKHPDQIANIEVPCVLFDGRS
                     GDFATEMFVIINSTHTRINRSHLVDLYEKVSWETPEKKFAAKVVNLLYAEDDSPLQYK
                     "
BASE COUNT         3099 a         4096 c         4672 g         3235 t
ORIGIN      
        1 agataatcgg caacagaggt cagtttcgcg acatggagga cgaggacgtc tgtggccttc
       61 ttgtcgagca tggccctcgc gatagcaagg gctgtggcct tcgatgttcg tgcgatcggt
      121 gtcctcttat tcgtacaagt gatgatgaag tatataagat tccaccaggg gcggcaatag
      181 atttgccacc gggagggctt gcttgaccct cgtgcggatg tcggaagccg acacctcgca
      241 cggaggcaat cggaggagga tcagatggtg ctctcctacg gcgatctcca aggtggtttg
      301 tcgcgccgca tccaggtcgg ccagcgactg tgcgggaagg ggaggcagga tggtcaggcc
      361 cgacagagaa ctgaatgcca ggccgggcct agagatgacg acaaatgaac ataggttgag
      421 aagtgccccg ggttcccgcc agctcgggaa gtcgagaaag gcatccagtc cgattaagaa
      481 gaagagagcg gcatctgtcc cgtattcttg ctgaagcagc cgtattgtat cgatggtgta
      541 tgacttgcct gggcggcgga actcgacatc ggaaatggaa aaaagtggat cagaggcaat
      601 agccaaccgg accatctcgt agcggtcctt tgcaggggcc aggctctggt ggggcttgtg
      661 tggagggtcg ctcgtgggaa tgaacaggac gcggtcgagg cggagcgcgt cacaggcctg
      721 acgggcaatg gtcagatggc cgttgtggac cgggttgaaa ctgccgccga agaggccgag
      781 gcggagcgta ccagagtgct gagtgccgag tgctgagagc cgaggttttg aatctgccga
      841 catcatgact cagtactcag atctcagcac tcacgtctaa agaatcacga gattgtcccg
      901 atggatgact tcctcatatt cttgcgggcc cagctgttgt tggatctccg ccgttcttaa
      961 gcccttgatc ttgccaagaa tatcggaaga gaaattcacg aggcctttgg cgaattcttt
     1021 gccatcctgg tcgaggcaac tgacggggtc tccgggttcg aagagaccgg tcacgttcag
     1081 aatgcctgat gccaggaggc tcttgccccg tttggctaag gcttcgacag ctccctggtc
     1141 gagcgtgatg gttccgcgcg ggcgaagggt gtagccgatc caatgtttgc ggctggtcaa
     1201 tcgtcgctct ttggcgagga agaggctgcc gccgtcgccg ccgctgagga ctgtgggtaa
     1261 aagccctgcc cgctgcccgt tgagaatgag tgttgccacc ccgtactcgc cgaccttctt
     1321 cgcagcgcgg actttcgttg ccatgccccc tgtgccttca aaagttgtcg agacaccggc
     1381 ccgccgctcg atgtcatcat tgatctcacc gatcagggga ataagctggg ctgaagggtt
     1441 cttgcgcgga tcctccgtga aaagcccgtc gacgtcggac aagatcacga gcagatctgc
     1501 atcgacgaga tgggcaacct cggcggccag gctgtcgttg tcaccgacac gaatttcttc
     1561 tacggccacg gtgtcgtttt cgttgatgat cggaatgacg ccgaaatcga tcagtgcggc
     1621 cagcgtatgt ctggcgttca ggaagcggcg ccggtcggcc aggtcctggt gggtcaggag
     1681 aatctgcgca accctcaggc cgagacgttc gaacgatttc tcataggccc acatcagccg
     1741 gctttgcccc accgctgcgg ccgcttgttt gacggggaga ctcttgggat agcccttgag
     1801 cgcgagtttc ttgatgccgg agacgatcgc gccggacgag acgatcagca gttcacgatc
     1861 gctggagcgc agggctgcaa tctcatcggc caagcgatcg attcgttcgg gttgcaggcc
     1921 gacctcccga gaggagacca agctgctccc gatcttgacg acgatccgtt tggcctgttt
     1981 taggatttcg tctcgcatgc tgccgtccga agttgttcga cttgctgacc gacaaaagcg
     2041 atcaattccg gaagtccttc tctcgtcgcg gcggaaatgg gaagacagcg gtagcggtga
     2101 cgcttgcagt aggattgcaa agttttgagc cgcgtgccgt ctcctgccgc gtcgagtttt
     2161 gtcgcgacga tggcgaaggg acgtttagcc agtgccggat cgtaagaagc cagttcgttg
     2221 cgcatgacct cgaaactggc caccggatct tccgttgccc agtctgacac atcgatgagg
     2281 tgcaggagga gggaggtgcg ttcgatatgc ctcaagaact gaatgccgag gcctttccct
     2341 tcgtgcgctc cttcaattaa tcccggaata tcggcgacca cgaagccacg atcctcgccg
     2401 tagcgcacaa cgcccaaatt agggatcagc gtcgtgaagg gataattggc gatcttggga
     2461 cgtgcggctg aaatggcggc gatcaatgtg gacttccccg cattgggaaa tcccaccagg
     2521 ccgacgtcgg ctagtaattt gagttccagg cgaagctggc gttcttcgcc ttccgtgccc
     2581 acttcgaagt gtgtcgggac acggttggtc gacgtcgcga aatggctatt accacggcca
     2641 cctttcccgc catgcgcgat cgtcgaggtt tcaccgtctg taaccaggtc agccaggatc
     2701 tctcccgtgt catcatcgta gacgacagtc cctaccggca acatgaccac gacgggtttg
     2761 cccctacgcc cagagcaatt ggagccgccg ccgggttttc cgtcttctgc ttcgtaatgt
     2821 ttctgatatc ggaggtcgag aagcgtcgtg aggcgatgtg aggccgtgaa gatgacgtct
     2881 ccgccgtttc ctccgtctcc gccgtcgggg ccgccgcggg gtacaaacat ttcccgacgg
     2941 aaactgcagc tgccgtctcc accccggccg gctttaacgg tgatacggac ttcatcgaca
     3001 aacatcgggc accgtccatt acgagaatta caaaagcgtg accgagagga gtcttgtggg
     3061 gcagaggggc cctacaagag atcctcaccg tgactcacgt ggtaacgaag gagaaaggaa
     3121 gtgcgcctgt tacgacgcta cgggcgcggg gtagacgctg accttctgtc gcgcgcgtcc
     3181 gccctcgaat ttgacgattc ccgtcacgcg ggcgaagagg gtgtggtctt tgccgaggcc
     3241 gacattgaaa ccgggaaaga acttggtccc acgctggcgc acgatgatgg ctccagcttt
     3301 gacggtttca ccgccgtatg ccttcacgcc gagatattgc ggattgctgt ccctcccgtt
     3361 tctagatgag ccgccgcctt tatttgttgc catggcagat ccttcttacg ctgtttcgat
     3421 gccggtaatg cgaagcttgg tgaaaccctg gcgatggccc ttggtgcgcc gataattctt
     3481 ccgccgctgt tttttaaaca ccatgatcga acgggtccgg ccctggtcga cgatttccgc
     3541 cttgaccttg gcgcctttca ccagcggttg tccgacaagg acgccctggt cgctgtggac
     3601 cagccgcacc tggcttaatt cgaccgtacc gccgacttcg cctggcagac tttcaacctg
     3661 gacgagggtc ccgttttcga cccggtattg tttgccgcct gtctctatga ttgcgtacat
     3721 aaacccatgc ctccgttaca gaacatggtt gtttatcaca gtggtttcca gggtgtcaag
     3781 gtaaaggagg ccctcccact caaaattata gggagagctt cgctttaagc tggcccaaac
     3841 tttggagtta acctgttcga ggactatgtt tggggggtgt ggtgcggata agctgaagcc
     3901 aaacaagtgg gagacccttt cggaggatct gagatatgaa agcatcaagc agagcccaag
     3961 tgaagaagac aacgagccgg tttatcatgc ggccctaccg ccggattcag acctggtatt
     4021 tgtcctatta catgagcggg gatctcgtgg gcaaaggggt tgtcaccaat ctttcctgta
     4081 gcggcatgag agtgttgggt gatcatgcca tgaggctggg aacggatctc accgtacggc
     4141 tcacgttgga ggaggacaaa cctccccttg agattgagcg ggtcacagtt caatgggtga
     4201 aggaggctga attcggattg aagatcgtca ggatcagccc ttccgctgcc acacgcatta
     4261 cacacctgct ggcctttcag gcccgtgccc atcgcggaga atcgtaggcc tgtatgctat
     4321 tgcctcgcgc gcagatgcgg catcctccgt ttgcttgacg cttcttccgg gccactgcta
     4381 tagtccgttt tcacatcatg gaacctcaag gcaaaatctg gatggacggt tcgttcgtgg
     4441 attgggcgga cgccaatgtc catgttttga ctcattcgct acactacggc cttgccgcat
     4501 ttgaaggcat ccgctgctac aagggaaagt cgggggcggc aatttttcgg ctccaggagc
     4561 atgtggatcg gttgtttgac tcggcccata tcggcatgat gaccatcccc tacgagaaga
     4621 aacaggtggc ggaggcaatt gtcgagacgg tccgggtgaa caagctcgat gcctgctata
     4681 tccgtccatt ggtctatatc gggtatgggg ccatgggcgt ttaccccggg gataatccca
     4741 tcaagttggc catcgccgca tggaagtggg gcgcctatct cggggatgac gccatcgcca
     4801 atgggatgcg cgcgcgcgtg tcgtccttta cccggcacca tgtgaacgtc tccatgacca
     4861 gaggcaagat ttctggctac tacgtgaatt cgattttggc gaagcgggaa gcgaaggccg
     4921 acggctatga cgaagcgatc ctcttggacc cggaaggcta tgtgtcggaa gggaccgggg
     4981 agaatatttt catcgttcgc cacgggagaa ttaagacgac ccccttgacc tcgatcctag
     5041 aagggatcac tcgaaattcc gtgatggatc tcgcacggga gcaggggatt ccggtcatgg
     5101 aagagcgctt cacgcgcgac gagatgtata ttgccgatga agtcttcatg acgggaacag
     5161 ccgctgagct gaccccggtt cgggaaattg acaatcgccg gatcgggaca gggaagccgg
     5221 gtcccatcac gcaggccctt cagaaacgct tcttcgccat agtccgcgga gaagatgcca
     5281 cccacgagtc ttggttgact cgcgtctaac gtatcaggct gaacaagcag attagtgtgc
     5341 cgtagaggaa tcgccggatg aatgcgattc tgctggaggc ttatcggccg gggcggccgg
     5401 tgccggttgc gatgtctctt tctttttgag gtcgataacg gtggaggaaa acgtcttttg
     5461 tttggcaagg atggcgaggc tgaacgaagt gaccatgaac acgatcgcga cgacgacggt
     5521 gaacttgctc agaaaattcg ccggaccgcg gctcccgaag accgtttggc tcgacccgcc
     5581 gaaggatgcc ccgatttcgg ctcccttgcc tgattgcaga aggatggcgc cgatcatgag
     5641 gaagcaggcg acgatgtgaa tgatgatcac gagcgtatgc atgggactcc aatcagtgaa
     5701 ttgatttatt ctccgccgcg gcgacgattg tagcaaacga atccgacttg agacaagccc
     5761 cgcctacgag agcgccgtcg atcccgtcga cacacaatag cgactcggca ttctgcggtg
     5821 tcacgctccc gccgtacaag attctcatct cggcagccgt gccctgattc gaggcgtttg
     5881 ccatacaagc tctgatggat cggtggacag caacggcttg agccgtggtt gccgcatgtc
     5941 ctgtcccaat ggcccagacc ggctcgtatg cgatggttac cacggagagg tcctgcgcag
     6001 tcaagccggc cagtgcagcg gtcagttgcg cggtgacgac cgcatccgtg cgatccgctt
     6061 cgcgctccgc gagagattct cccacgcaga ggattgggcg caggccatgc accaatgcgg
     6121 cccggacttt tttctgaatg tcgccgtctc gttcgccgaa gagcgtccgc cgctccgagt
     6181 ggccgacgat cacataggtg caacccaagt cgcgcagcat cggtccggac acttcaccgg
     6241 taaaggcgcc ttgagattcg ccatgaaggt tttgcgccgc aagtttgatc ctggacgagg
     6301 ggcccaaggc ggtacgggcc gattcgaggg cggtaaaggg cggagcaatg acaatctcgg
     6361 cgcgggaact gctcgccagt tttgccgcta attcgcggac gaacccggcc gcttccgagg
     6421 ccgtcttgtt catcttccaa ttgccgacaa tgaggggtat gcgcacggtg tctcgatggt
     6481 cagtggaaaa cttgccgcga tagccggcgc tagtccttgc gttccggcag ggccgtcaaa
     6541 ccgggcaact gcttgccttc gagcaattca agtgcagcgc cgccgccggt tgaaatgaag
     6601 gaaatgctct ccgattcgcc ggctcgatgg acggccatgg cggtctcgcc gccgccgacg
     6661 atcgtcagcg cataggcgtt ggcgaccgcg tgagcgatag acagggttcc acgagcatag
     6721 gcatcgattt caaaaactcc catcggtccg ttccagagga tggtcttggc gttttggacg
     6781 gcttcggtaa acagtttgac cgaagcggga ccgatatcga gcgcatacca gcctttgggt
     6841 atttcctgga cagggacgat cttggtttcc gcgccaggct cgcggctggc ggccaccacg
     6901 caatcgaccg ggagatagaa cttgaccccg cgtgaaagtg cgtggtcctc gattcctttg
     6961 gcaaaatcga gcatatcgtt ctccacgagg gagttcccga tttcgagccc cttggctttg
     7021 agaaaggtga aggccatgcc gccgccgatg atgaccttgt ccacccgttt gccgaggttt
     7081 tcgatcacgc cgatctttcc ggacacctta gcgccgccga gaatggcgac gaacggtcga
     7141 accggatttt ccaccgctcc ctcgagatac tcgatctcct tcttgaggag aaagccggcc
     7201 gccgctgcgg gaatgaattt cgtaatgccg acagtggagg catgagcacg gtgcgccgcg
     7261 ccaaaggcgt cgttgatgta gatgtcgcca agtgcggcaa gggacttgga gaatccctcg
     7321 tcgttctgtt cttcgcctag atggaatcga aggttttcca gcaacatcac gtcgcccggc
     7381 ttcatcttgg cgacgagacc ctcgatcgcc gggccgatgc aatcgggcgc gaagatgacg
     7441 tctttgccga gcaggcggcc taaccgtttg gcgatcggag ccaggctgta tttcggatcg
     7501 aatttccctt tcgggcgtcc gaggtgcgaa cacaaaatca ctttgccgcc gtcgtcgacc
     7561 acgcggttga tggtcggcag tgtggagcgg atgcgggtgt cgtcggtaat ctgcagcgag
     7621 tcgtccaggg ggacgttgaa gtccgcgcgg atgatgaccc gtttcccgga cagggagaga
     7681 tcgtcgatcg tctgcttatg caaattcatc cttcggccct cacatcagcg gaggtcagcg
     7741 tgaagcggtg agcgctctgc agtgatatcg gtctatcggt ccttggcttt gccggcaatg
     7801 accttgatca gatcacgcac gcgacaggaa taaccccatt cattgtcata ccaggcggtg
     7861 actttgacga gccgcttgtc gatgaccgta gtcagcggcg catcgagcgt ggccgagtgg
     7921 gggttgccct tctggtcgat agagacaatg ggatcttctg aatactggag aatatttttc
     7981 aatggaccgt ttgccgcttt cttgaacgcc tcattcacgc cggccacatc gcaatccttt
     8041 tcggtctcca ccgtcaagtc gaccagcgag acgttcgggg tcggcacgcg gattgcgagg
     8101 ccgtccatct tgcccttgag ttgggggatg accagatgga gcgctttggc tgcgccggtg
     8161 ctggtcggaa tcatcgacat gccggcggcg cgtgcgcgac gaaggtcctt gtgcggaaga
     8221 tccagcagct gctggtcatt ggtataggag tggatcgtcg tcatcacgcc gtgcttaatg
     8281 ccgaagcttt ccatcagtac tttggcaact ggcgccaggc agttggtcgt gcaggaggca
     8341 ttggaaacga tgtggtggat tttgggatcg agcgtattgt cgttgacgcc caacacgact
     8401 gtcgcgtccg ggtctttcgc cggggcggaa atgatcacgt acttggctcc ggccgaaagg
     8461 tgtttaccag ccccttcccg atcggtaaaa cggccggtcg attcgatgac gatatcgacg
     8521 ttgagatcct tccaggggag ttctttcgga tccttgacag cgaggacctt gattggtttg
     8581 ccgtcaacca gaatttggtc gtccttggcc tcgacgcttc ccttcaaggt accgtgtacc
     8641 gagtcgtact tcagcaagta cgcgagtgtt ttggcatcgg tgagatcgtt gatcgccacg
     8701 aactgcagtt ccgggtcgcc gagcgaagct cgcagcacgt tgcgcccgat tcggccgaac
     8761 ccgttgattc caactctgat agccatagta agagagtccc tccaagaaaa aaacaagccg
     8821 gtcattaaca ccggccgcga cagaccgcga tagtatcgac caccccagac cttgtcaagt
     8881 gatacggaag tggtaactcc gcgtaggtag agaggtttcg agtccccatg gccatgaatc
     8941 tacgtaacgg gctgagaggc gggattctct gtcttgcgtc tccgatggcc ggttcgctag
     9001 agtccagccc tcactctgtt acgggattag gccatgaggt catcgtgaag ctcttcgaac
     9061 aaacgacgca agtgttggaa tggggaaaac tgctggaggt tcttgcctcg catggccgtt
     9121 cgacgatggg agcagcccga tgccgggctg cggttctgcc gactgatctt gtcgaagcgc
     9181 aacgacgtca ggaggaaacg gtggaggtag ccaggctcca ggagaccgcc gaccctccgc
     9241 cgtcgttgtc gtttcccgat atcagggagc cgctcgcacg cgcagcaaaa ggcgctgcct
     9301 tggacgcgca tgagctgcgc gatcatgcgc ttgttcttgc cttatgggac gaggttcagc
     9361 gttatctcag ccgccatgcc caggaggcgc cgacagtggc cgcagcggcg gaggtcctac
     9421 gatcgggacg ggagctaaaa gaactgagcc gcgcgttgga tggcgcgatt catccggatg
     9481 gttcgatcaa ggaatcggcc acgccggaac tgcggcggtt tacgcaccac gccaacgact
     9541 taaagcagca gattcgccat cagctcgatc aaatcctaca gtcgcggcgc tttgcagagt
     9601 tgctgcagga acagtatttc gcacagcggg aaggtcgcta tgtcgtgcca attaagacag
     9661 aaatgcaggg gcgcgtgcca ggtattgtgc acgacgtctc gtcgagcggg gcgacggtgt
     9721 tcgtggagcc gcgtgaactt gtcgaactga acaacgcaat taaggtggcc gacctcgaga
     9781 tcgatcggga gatccgcaga attctccgcg aactgtccag cctcgtggcg gagaagtccg
     9841 atctgattct cgccggcctt gacgtgctgg cggagctgga ttgtatcgcc gcgcgggcag
     9901 ccttcgggcg acaactgcga gcccatccgg tgggattgaa cgatcacgga cgcgtgcgtc
     9961 tgaagcaggc gcggcatcct ctgctggtgc tctcaaaagc tcaggtggtc gccaacgaca
    10021 ttcttctcga ggaccatgtc caggtcctgg tgatctccgg tccgaacacc ggcggcaaaa
    10081 cagtcacctt gaagatcgtc ggtctctttg ctctgatggt tagagccggt ctgctgctgc
    10141 cctgcgagcc ggagtccgag atggcgatct tccaggacat ctatgctgat atcggcgacg
    10201 cgcaggatct ggcccgtgat ctctcgagtt tctccgcgca catgacgcag atgattcact
    10261 tactgaagga cgcgcatcgc gcccggcagg cactggtgtt gctcgatgaa ccggtgacgt
    10321 caacggatcc gattgaaggt gcggccctcg ccgaggcgtt gctctgccgg cttgcttcgt
    10381 tagggatgaa agtcatcgcg acaacgcact accggtcgct caaagccttg gctcagacga
    10441 cggccgggtt taccaacgcc tcggtggaat tcaatgtggc gacactttct cctacctatt
    10501 gcttatttat gggcgtgccg gggggatcct cagcgatcga aatcgcgggg cgactgggga
    10561 tggatcgtgg cttgctcgac gaggcgcggc agaagttgga gcaggacgat cgggtattgg
    10621 aacggatgct cgacgatttg cacgggaagc agcggcaact cacagaggac ctggcgcggg
    10681 ccgttgcggc cagagccgaa gccgaagagg ctgcccaact agccaaagac cagctggctc
    10741 ggctggaagc cacggagcgc gaggaacgga aaggcattaa gaagaaactg caggaacagt
    10801 tcagccgtgc ccgggccgaa gtgcaggcga cggtcgagga agtcaaacgc gagcagaagt
    10861 tgatcaaggc aaaagaggcc aaacaacggc tggtcgaatt ggaggcgcag acgagggccg
    10921 aattcactcc ttccggcacc ccgattccac tcgaacaact cggggcgggt gatcaggtcg
    10981 agattggcag attaggaatg acagggacgt tgcttgaagc tccacaaggc aagaagcgcg
    11041 tgcgtgtcaa agtcggcgag ggtgaattgt tggcgactgt cgctaatctt attggtttgg
    11101 ctcgagggac gacctcgttg ccggccgcag tccagtcggc accgtcgaaa ccgttaggct
    11161 cgactcgtcc ctatcatgcc gaagaacaga ctgtcgtcga tgtgcgaggg aaggcggcgg
    11221 atgatgcgct cgacgaggtg gtcgccgcac tggatcgcgc cacactcgaa gcgacgcccg
    11281 cactgcgcat tattcacggt cacggaaccg gaaaattgaa agccgcgctc cgcgaatatt
    11341 tgaagagctc cccttatgtc gccagcgcac gccctggcga tcgagccgag gggggcgatg
    11401 gtgtaacgat tgtcacggtt cggtagccga gtcggtctac tggatgtggt actttgccat
    11461 gcggtagcgg agcgtattgc gggtaatctt gagcaggcgg cttgcttctg agacgtttcc
    11521 tgccgctttt cggagcgctt cttcgagcag tgtcttttcc atgtcttgaa acgacaggcc
    11581 gaaggagagg agcgacggtt tttccgccga gttactgagc gcagactgtt gccctgcccg
    11641 gaatgaggtt gccaaatgct ccgggcgaat tgtctcgtct ttgcaggtaa tggtcagtcc
    11701 ctccaccaca ttgtgaagct cccgcacatt gccgggccag tcgtagcgct gcaacagggt
    11761 caaggcagcc ggggttatgc cgctgatccg gcagccatgc gcccgccggg caaaatccaa
    11821 gaacgacatg accaggggtt cgatatcatc caatcgttca cgaagcggtg gaatgcggag
    11881 ttgataaacg ttcaaacgat agaagaggtc gaggcggaat cgcccggcct tgatgtttgc
    11941 cggcaggtct tcgttcgtcg ccgcgatcac gcggatgttg accttctggc tctgtgtgcc
    12001 gccgagcgga tcgacgacat ggtcttccag cacacgcagg agttttgcct gagcggtcaa
    12061 gctcatctcg ccgatctcgt ccagaaataa ggtgccgccc tcagcgagct gaaagcggcc
    12121 tggtttgttc tgcgtggcat cggtaaaggc cccgcgttgg tagccgaaga gctccgattc
    12181 caggagatgc tcgggaatgc cggcgcagtt cagtgcgata aagggggctt ttgcgcgagg
    12241 gcttgtcgcg tggatggctt gcgaaaagag ttctttcccg gttccgctct cgccggtaat
    12301 cagcacggtg gcatcagtca atgcgacttc ttccgccagg tgtttgacca agcgcatttg
    12361 cggcgagacg ctgacgattc gatcgaaccc ctgtggatgc tcacggaccg gcggctcgct
    12421 cgtagcgtag taggcttcgg cctgttgaag cgcccgacga aggacctcga ccataagggg
    12481 tttggggaga tagttggtgg ctccttgatg catggcctcg acggcggctt tggccgagtc
    12541 ctcttttccc agcacgatga tcgggacagg ccgtgccttc tgccttgcct gagccagcac
    12601 gagctcgcga gcaatctgcc ctgcaccgtt gtcacagacg atcaaagaaa gatcatgccg
    12661 tgttctgatc aagcgaaggc cctcgtcggt ggtcgctgct gtcacgaggg tggtgttggg
    12721 gttcgagaag gcctgttcga ggagcggtcc tgtggaaagg tcggctgaga tcagagcgat
    12781 gatcggagga tgcataggag tggtcctttt tctagcttcc tgctaaccac cgaataactc
    12841 aattagccta ctcctctttg tggcgagaag aaaaggggga gaagaagggc tgaggagtca
    12901 atggtgagtg ctgagatgga gggcctagga ttgattctgt cgggcgatgc ggagatttcg
    12961 ctgaaaacct gtgtatttgg cacgacgaat cgggctgtcc ttgaaggttg tgtggaagct
    13021 ccgctcgtcc atgttgagca actcgtcaag gcgaggagct tgggtcagac cgcttggtgc
    13081 gaaggcgggt tcgcttgtat ggtctgcgcg caagttgaac gggcagatgt cgaggcaatc
    13141 gtcgcatcca aagatgtgat ggcccatctg ggaaggcagt tcatccggaa tctcttcccg
    13201 agttccacga aattcgattg tcagatagga gatgcagcgg ttggcgtcga cgacgtaggg
    13261 ctcgacgatg gcgccgcttg gacaggcctg aatacagagg gagcagcttc cgcaaagatc
    13321 tgtgcccggc tggtcaggcg gcaggtccag tgtcgtgaga atttctccca acagcagcca
    13381 cgaaccccat tcggaagaca cgaggttgga gtgtttcccg atccagccga gacccgcctc
    13441 ctgggcccag gctttttcca taatcggtcc tgtgtcgacg taggatcgcg tcaggtttcc
    13501 cggcgccaga tctagaatgc gggcttccaa ttgtttcagc cgatcgccaa gcacctgatg
    13561 gtaatctttc ccccaggcat accgagccac gcggccgtat ccgggacctt ctgttggccg
    13621 atgccccgtg tcgtagttca tcccgaccga aatgagcgat cggcagccag gcagtaccag
    13681 ccgtggatct gtgcgccgcg acggctcgcg ggctatccag gccatcgtgc cgtggtaacc
    13741 acgccggagc cattcttgga ggcgaaggta aagacgtcgg ggcagagaat ccgtgtcgct
    13801 cttgtctgtc gaaggaggag tgcagtcggt gattcggcag atgccgacgg cactgaagcc
    13861 cagtgcgcgg gcttcccgct taatcgcttc cgcgagcgac atggctagta ggtttcaaag
    13921 gaacagtata gatcggaaac aagatggaaa ggccgcataa ccaggctcgc acagcatccg
    13981 ctaatggggg ttgcagtcga atcggacaat gaactgaacc gaacaatgtc cagtgctgca
    14041 gcggccgcag gtgccggtga tcttggtttt ccagtccgtt gctttttcgt caaaccggca
    14101 aaacgtcttg ccacctttgg aaatcgtcgt ccagggcttg ttggttcccg gtgtgttggc
    14161 gacgaggcag ccggcaggga tcggaacctg gcaatgctga gagggttctc ccttcgccat
    14221 gccgaaactg caggattgct ccgtcgtggc cggaaaatcc gacggggcct gggcgccggc
    14281 ctctgagggt tgatacatca tcaggccgat aagcggcaac aggcaaacga cagcgcgcaa
    14341 gatgagcata gtcatgccgc ggatggtaac acaggccccg cgcttgcggc taccggcatg
    14401 agtgcgtaga atcttccgag cagtcgtttc gtcatagggg aggcactcat gcttgcgacc
    14461 cggattcgtc aaaaggaagg gaccttttac ttcgttgcct acaatgcggt cgatctgttg
    14521 gagaaggttc gcttcacgag ccggtatttt tttgaaggcg aagaaattgc tcagggaaag
    14581 atttccgaac acgacgaagt ggcgcagttc atcgcgggta tcgagcgcag cgaaaaaggg
    14641 tttcagcgcc tcctgaaccg gcaaaaaatc aaacagatcg tgaatttcta tgaaacggtc
    14701 gtggcacagc cgatgatccc ggggacggtg ttgatcttta cggatgagac cttgcgtttt
    14761 cagcgggcgg gggagtccga atccatcggc catttgagcg agccgaaggg aaagtatctt
    14821 gtcatcgatg gacagcaccg gctggcgggg ctgcactttt ttcgagccaa gcacccggat
    14881 caaatcgcca acatcgaagt gccctgcgtc ctgtttgacg gccggagcgg ggattttgcc
    14941 accgagatgt tcgtcatcat caacagcacg catacgagaa tcaatcgttc gcatctcgtt
    15001 gacctgtacg aaaaggtttc atgggagacg ccggagaaga agttcgccgc gaaagtggtg
    15061 aatctgctct atgccgaaga cgattcgccg ctgcagtaca aa
//
LOCUS       DATIFR010000150        16637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1821745, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000150 DATIFR010000000
VERSION     DATIFR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16637
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821745"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="VLD60_09095"
     CDS             <1..2153
                     /locus_tag="VLD60_09095"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSB45156.1"
                     /translation="LHLLPTLWFRNDWAKWIAGPAQKPTLKKIQGPAGTSAIAAQHRT
                     LGTYHLYCEGDVPLLFAENETNNARLFPQFSNPTPYVKDGINDYVVQGRQAAVNPAHE
                     GTKASAHYRQMVGSGKSMIVRVRLTSQGPVEQGKNKKGVAAPFGPAFAETVAVRLKEA
                     DEFYRSVTPPSVSPDAANVMRQAIAGMLWSKQYYYFDGDRWLEEHHSNPLHAGYQNAR
                     NSEWFHMINEDIISMPDKWEYPWYAAWDLAFHTLPLSIVDPDFAKQQMHLMLHGRYLH
                     PNGQVPAYEWNFSDVNPPVHAFATLFLHRTEQALRGETDVEFLRAAFHKLLLNFTWWV
                     NRKDRFGKNVFEGGFLGLDNIGVFDRSAPLPTGGHLEQADGTAWMALFSQNMMELAVE
                     LTGHDPTYEDMVTKFLEHLLYIAGAMNKPGPDGMWDEEDGFYYDLLRLPDGGTQRLKV
                     RSMVGLLPLCATTVTEPWQREIIPETMAALQARAFQMPELFKSIHPTGPKHLGVAERG
                     IIALLNPDRLRRILSKMLDENEFLSPYGIRSLSKFHEQHPYIMHVGGQEYRVDYLPAE
                     SNTGMFGGNSNWRGPVWMPVNVMIIRALLNFYLYYGDSFKIECPTGSGKMMNLFEVSK
                     DITDRLNRIFLRDGRGKRPVYGGAEKFQSDPHWRDHILFYEYFHGDNGAGLGASHQTG
                     WTGCVAKLIQLYGSIDAKQALEGGKRAMFKKRGA"
     gene            2150..3631
                     /locus_tag="VLD60_09100"
     CDS             2150..3631
                     /locus_tag="VLD60_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSB45157.1"
                     /translation="MKAWRKYPVIYEINTWVWLSELSRKHRRPVALATVPDEDWDAIA
                     AYGFDAVWLMGVWERSPAGTAISMKNEGLLTDFRHALPDFVPEDNVGSPYCVRRYVVD
                     QSLGGPEGLAAARKKLAKGGMRLILDFVPNHVAPDHPWVTEHPDYFIQGSSDDMAQKP
                     GEYFQAGGKVLALGRDPYFAPWPDVAQLNGFHPGLRKAIIETVGSIAGQCDGMRCDMA
                     MLLMTPIFESTWGPRAGPRPAREYWSEVIQGVRKRHPDVLFMAEAYWDKEWELQQQGF
                     DYCYDKRLYDRLEHGQAESVRLHLCADLPYQEKLVRFIENHDEPRAAATFADKQAQAA
                     AVTFATLPGARLFHEGQFEGRRVRLPVFLRRRPDEITDRSLQEFYNSLLQTLRSAAVR
                     DGEWTLCERTGWPDNDSFLNVVAWCWQKGADRHLIVVNLSGVRSQAQVKLPWGDLAGR
                     SWRLNDPVQGTVYDRAGDELHAWGLYVDLEPWGYHVFTVSSGT"
     gene            3654..4028
                     /locus_tag="VLD60_09105"
     CDS             3654..4028
                     /locus_tag="VLD60_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HSB45158.1"
                     /translation="MVPSKRLQWLGFFFCVMILAGCAELAQDAESLIHSSLRRSDVVG
                     FVAGFGTTFAALPDLIAMLKRRSSKGMNPRMAAIMGAFQILWVWYGLLIVSRPVVLWN
                     IVAVTTNFISVAAYFYFARREH"
     gene            4166..4366
                     /locus_tag="VLD60_09110"
     CDS             4166..4366
                     /locus_tag="VLD60_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45159.1"
                     /translation="MDVLRDMGVRRIAISGLFLVAVFALDLVTPLGIPVWLLYGVPFL
                     FLQTYSPRYHVHGLAIVCTFLI"
     gene            complement(4551..5891)
                     /locus_tag="VLD60_09115"
     CDS             complement(4551..5891)
                     /locus_tag="VLD60_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSB45160.1"
                     /translation="MSELQIYITMAVFGAVILAIAFDVIDMAVATLLGVSVLFGFNIL
                     AEDDVIAAMRTAGGPLSLLFGGMVVARTLASTGIFDRIGASYLHATKGSGKRFLLLLV
                     ALVAPLCAFLPNATTVILLAPIIIRVATALEVDFVGPMVLTAMVSNSAGLLTLVGDPA
                     TFLVGSSIGMSFGQYLHKVSLGGLLSLLVLIPLLPLLMPDLWRLQRQLPPQVTEKRFQ
                     RRWFALLAFAVLAIMIGLFLFGEDLPDPIGPPAVAIISASLALLVIYGAKIEPIDNVL
                     RDIDWKTLVFLGCIFFLVQAITKTGLLQSFSLKLYDWFGTEFWLAALALIAGIGLLSS
                     LLANIPVVAASIFMVKGYLVAVEAVPEAALGASFTDWPVAIIPVFVAMMFGATLGGNA
                     TLIGASANIVSVGICARNGKPVTFITWLRYGLPITACQLAVSALYVLVLFYFMR"
     gene            complement(6182..7219)
                     /locus_tag="VLD60_09120"
     CDS             complement(6182..7219)
                     /locus_tag="VLD60_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSB45161.1"
                     /translation="MPVSAVARILFPAAVLFAVVWAVSPAAAGDTLGQIKARGTLHCG
                     VNQAKPGFSTKDASGQWSGMEVDFCRAVAAAVFGDPKKVTFVPLRASERFPALKSSVI
                     DLLARATAWTLSREASLKLRFPGILFYDAQALMVPAAEGIKSVKQLDGATICVEKGTT
                     HERNLTDHFTAQGLRVKPLVIDSPTEVADALFSGRCKAYTSDISDLAGARLRAPAGQT
                     FDILPEHLSKEPLGPVVRSGDDEWLTLVRWVLFGLIAAEENGITRDNIREIRETTRHP
                     AARRALGADGGFGPMLGTDQDWALHAVESVGNYGEIFERNLGVHSPLHLDRGLNRLWT
                     HGGLMYAPPVR"
     gene            7796..9028
                     /locus_tag="VLD60_09125"
     CDS             7796..9028
                     /locus_tag="VLD60_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB45162.1"
                     /translation="MNAQMLGVIILCGTMLAGCVTKSTHEATLTELDQAQKASSKMAA
                     DFDSFKKQSAAQIAALEADKSQLSNDLLNSQTASAKLQRELDKARSSLDTTLASRNDM
                     EQDVRKIRAESSGMERLNGELRREREQLQDKLDALQKNYDATKQDLETKLKAEQDTVA
                     ALQNDKQLLIARATGATDELAKLQKRMGELETDASRAKDLEQRLSQRDIELGKLTQGG
                     ETLAAEKERLAAEKERLEKERAAKQAEIDRLTKTQQELTKSLQAEIERGDITIKQVRD
                     RLTINMVEKVLFNSGQAQVKPAGLKVLTQVSDVLKNVTDKQIRIEGHTDNVPIGVKLQ
                     EKFPTNWELSTARATNVVRFFIDRGGVNRERLEAVGFADTRPVAGNETEAGRTDNRRI
                     EIVLYPKDLSEIVGEAKP"
     gene            9074..11137
                     /gene="glgX"
                     /locus_tag="VLD60_09130"
     CDS             9074..11137
                     /gene="glgX"
                     /locus_tag="VLD60_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSB45163.1"
                     /translation="MQTTGNSAPLGATYCHGGVNFSLYSREATGVELLFFDRLDDAKP
                     ARVIGVDPVSNRTYHYWHVFVPGVRPGQIYGYRAHGPFDPANGLRFDPTKVLLDPYGR
                     GVVVPKKYSRDAASEGGENTATAMKSVVVDASTYDWEGDVTLRRPSARTIVYEMHVRG
                     FTRHPSAGVAENMRGTYAGLIEKIPYLQELGITAVELLPVFHFDSQDCPPGRINYWGY
                     APISFFAPHPAYSSRQDALGPVDEFRDMVKALHRTGIEVILDVVFNHTAEGDQHGPTL
                     SFRGLDNNTYYILEQDRSRYANYTGTGNTLNANHPIVRRMIVDSLRYWVEVMHVDGFR
                     FDLASILARDVSGCVMPSPPVLWDIESDPALAGTKLIAEAWDAAGLYQVGSFIGDSWK
                     EWNGRFRDDARSFFRGEEGSLARFADRMIGSPEIYGHEQREPEQSVNFVTCHDGFTLN
                     DLVSYNEKHNDANGEDNRDGANDNRSWNCGVEGPTNDPDVEKLRNRQVKNFLTATLLS
                     IGMPMILMGDEVRRTQRGNNNAYCHDNELNWFDWRLVGKHADVHRFVTLLNERRMLRA
                     VEHERQRLSLVQLIQQANKAWHGVKLGQPDWSPHSHSIAFSVEMRKEPLLVYMILNAY
                     WEPLDFELPPVQNDRAILWKRWIDTALDSPHDIVPWQTAPSIPGYSYRAESRSVVVLF
                     ANLTG"
     gene            11396..12211
                     /gene="ppk2"
                     /locus_tag="VLD60_09135"
     CDS             11396..12211
                     /gene="ppk2"
                     /locus_tag="VLD60_09135"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HSB45164.1"
                     /translation="MAKDDGQEENGKGKLKRKEYEKELRRLQAELCHLQAWVKHKGLR
                     IMVVFEGRDGAGKGGTIRAITERVSPRIFRLVALPAPNDREKTQMYLQRYMAHFPAAG
                     EIVIWDRSWYNRAGVEHVMGFCTKEQYEGFLEHASIIEGYITRAGIILIKYWLEVSNK
                     EQERRFEARVEDPLRQWKLSPMDLPSREKWYEYSRARDRMLEATDSKHAPWHIVLSDN
                     KKSARLNCITHLLSVIPYKKLPREKIKIPKRSNKYKYDDQASLKGRRFIPEVY"
     gene            12396..13097
                     /locus_tag="VLD60_09140"
     CDS             12396..13097
                     /locus_tag="VLD60_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HSB45165.1"
                     /translation="MPIVTLHQRWEQILENRCFFLFLAMLILLVSMPFLGETVHGRLI
                     IGVINITMLMTAVAAVGRSRFSLLFSFILAVPTLTFQVLALQSGDAGHFALSWSCNAV
                     FYSLTLTRLLHYVLRKDTITADKLYGAIAAYILIAILWGFLYGVLQYFYPGAYALGGT
                     PKVLNMGDLIYFSFTALSTAGFGDITPLLIQSRFLTILEMITGVMYVAILIARLTGVY
                     PIVGTKPPDTGGQAG"
     gene            complement(13269..14741)
                     /locus_tag="VLD60_09145"
     CDS             complement(13269..14741)
                     /locus_tag="VLD60_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSB45166.1"
                     /translation="MKLVSIGVVCICLLLSLSAFAEEKQTSKKHELTQAPALKPWTGD
                     LDGMVKRRVIRALVSPSRTAYWLNGARQIGAEYELLKAFENELNNNYKTQGKHIRIVV
                     AIIPTRHDQLISGLLEGRGDIAAGILTVTPERLEQVDFGEPFFRGVKQIVVTGPASPE
                     LASIEDLSGKEVFARKSSSYWTHLERLNERFAKEQKPPVILKAAPEDLQDDDLLEMVN
                     AGLVSIVVVNRYQGLLWAKVFKKLKPREDLVINEGGDIAWMFRKDSPKLKAEIATFAK
                     NYGMKSDFGKSLIKKYAASTRFVKPASSASEIKKFEQTVEFFRKYGARYDMDYLLMMA
                     QGYQESLLDQNARSDAGAIGVMQLMPATGKEMDTGDITQVEPNIHAGVKYISLMRNQF
                     FADQPMDMRNKTLFAFAAYNAGPGKVLKLQKEAEKRGFDPHVWFNNVEIIAAQRIGEE
                     TVIYVANIYKYYVAYTLIEEQRAQKEKARSELLQNAPKQP"
     gene            complement(14989..15711)
                     /locus_tag="VLD60_09150"
     CDS             complement(14989..15711)
                     /locus_tag="VLD60_09150"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSB45167.1"
                     /translation="MRRIASVLLIGLLVGIGGCAATQEASKVEKSGFLGDYSILKEGE
                     RSTFKEGAQDTALWVYKNPAADWRKYKKIQLDPVTIWMSQKDSQLKDVPVEDRQRLAA
                     LLWSKLDEQLRKDYEMTSQGGPDVIHMQAAITEAGESNAVLDTVTSIIPQTRLLTGAK
                     GMATGVSGFTGSASAELKVTDGETGTLLLAAVDRRGGTKSLSGMTSSWHDVEEAYRFW
                     AEKTRYRLCQFRGGTNCVEPNP"
     gene            16215..>16637
                     /locus_tag="VLD60_09155"
     CDS             16215..>16637
                     /locus_tag="VLD60_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB45168.1"
                     /translation="MMSSPDSDPTHASGFSTPVGTRRKFFQWVTRAAAGFIGLGLAVP
                     LLGYVISPALKRRDKPWVEVGGVDQLTAGEPKQLDYVATIHDGYLELTTHKAVWAVKQ
                     SDGQVTVFSPMCTHLGCGYHWDVTDRKFKCPCHGSVYDV"
BASE COUNT         3519 a         4720 c         4963 g         3435 t
ORIGIN      
        1 agctccacct cttgccgact ctgtggttcc ggaacgactg ggcaaaatgg attgccggac
       61 ctgctcagaa accgaccctt aaaaagatcc agggaccagc cggcacgagt gcaatagcgg
      121 cgcagcatcg tacgctgggc acgtaccatc tgtattgcga aggcgacgtg ccgctgctct
      181 ttgccgagaa cgaaacgaac aatgcccggc tctttcctca attctcgaac cccaccccct
      241 acgtcaaaga cgggatcaat gactatgtgg tgcagggccg ccaggcggcc gtgaaccccg
      301 cccatgaggg gaccaaggcc tcggcgcatt accggcagat ggtgggctcg ggcaagtcga
      361 tgatcgtgcg cgtgcgcctt actagccaag gtcccgtcga acagggcaag aacaagaaag
      421 gggttgccgc ccccttcggg cccgcgtttg ctgagacggt cgcggtccgc ctcaaggagg
      481 cagacgagtt ctaccgttcc gtcaccccgc cgtccgtctc cccggacgca gccaacgtga
      541 tgcgtcaggc cattgccggc atgctctggt ccaagcagta ctattacttc gatggcgatc
      601 gctggctgga ggagcaccac tccaaccccc tccatgccgg atatcagaac gcccggaact
      661 cggagtggtt ccacatgatc aatgaggaca tcatctcgat gcccgacaag tgggaatacc
      721 cctggtacgc ggcctgggac ctggccttcc acacgctgcc gctctcgatc gtcgacccgg
      781 acttcgcgaa gcagcagatg cacttgatgc tgcacgggcg ctacctacac cccaacgggc
      841 aggttcccgc ctatgaatgg aacttcagtg acgtgaaccc gccggtccac gcctttgcga
      901 ccctcttcct ccaccgcacc gagcaggcct tgcgcggcga gacggacgtg gagttcctcc
      961 gagcggcctt tcacaagctc ctgctgaact tcacctggtg ggtaaaccgc aaggaccggt
     1021 tcggcaagaa cgtgttcgag ggaggattcc tcggcctcga caacatcggc gtcttcgacc
     1081 ggagcgcccc gctgccaacc ggcggccacc tggagcaggc ggacggcaca gcctggatgg
     1141 ctctgttcag ccagaatatg atggagctgg cggtggagct gacgggccac gatcccacct
     1201 acgaagacat ggtgactaag ttcctggagc acctcctcta tatcgccggg gccatgaata
     1261 agcccgggcc ggacggcatg tgggacgagg aggacggctt ctactatgat ctgctgcggc
     1321 tgccggacgg cggtactcag cggctcaagg tacgctcgat ggtggggctc ctgcccctgt
     1381 gcgccaccac cgtgaccgag ccgtggcagc gcgaaatcat tccggagaca atggccgcgc
     1441 ttcaagctcg cgcgttccag atgcctgaac tgtttaaatc tatccacccc acgggcccca
     1501 agcatctggg ggtggcagag cggggaatca tcgctctgct caatccggat cggctccggc
     1561 ggatcctttc gaagatgctc gacgagaacg agttcctgag cccctacggc atacgctcac
     1621 tctcgaagtt ccacgagcag catccgtata tcatgcatgt cggaggccag gagtaccgtg
     1681 tggattatct accggcggaa tccaataccg gcatgttcgg cggcaactca aactggcgag
     1741 gaccggtctg gatgccggtg aacgtcatga tcatccgggc gcttctgaat ttctatctct
     1801 attacggcga cagttttaaa attgaatgcc ccaccgggtc cggcaagatg atgaacctct
     1861 tcgaggtttc caaggacatc acggatcggc tgaaccggat ctttctgcgg gacgggcgcg
     1921 gcaaacgtcc tgtgtatgga ggtgcggaaa agttccagtc cgatccccac tggcgcgacc
     1981 acatcctgtt ctatgaatac ttccacggcg acaatggggc gggacttggc gcgagccacc
     2041 agaccggctg gaccgggtgc gtggcgaaac tcatccagct ctacgggtcc atcgacgcca
     2101 agcaggccct ggaggggggc aagcgggcca tgttcaagaa acgcggcgca tgaaggcctg
     2161 gcgcaaatat cctgtcatct acgagatcaa cacctgggtc tggctgagtg agttgagccg
     2221 gaagcaccgg aggcctgtgg ctctcgccac ggttcccgat gaggattggg atgccattgc
     2281 cgcatatgga tttgatgcgg tctggctcat gggtgtgtgg gaacggagtc ctgccggcac
     2341 cgccatttcg atgaagaacg aggggctgct tacggatttc cgccacgccc tcccggactt
     2401 tgtgccggag gacaatgtcg gttcccccta ttgcgtgcgg cgctatgtcg tggatcagtc
     2461 cctgggagga ccggaggggc ttgccgctgc cagaaagaag ctggccaagg gaggcatgcg
     2521 actgatcctg gatttcgtcc ccaaccacgt ggcgcctgat catccctggg tcacggagca
     2581 tccggattat ttcatccagg gcagcagcga cgatatggca cagaagccgg gcgagtattt
     2641 tcaggccggt ggcaaggtcc tggccctggg gagagatccc tactttgcgc cctggcctga
     2701 cgtggcgcag ctcaacgggt ttcatccggg attgcgcaag gcgatcatcg aaaccgtggg
     2761 atctattgcc gggcaatgcg atggcatgcg gtgcgatatg gcgatgctgc tcatgacgcc
     2821 tatcttcgag agcacgtggg ggccacgcgc cgggccgcgt ccggcgcgag aatactggtc
     2881 ggaggtgatc cagggtgtgc ggaagcgtca tcccgatgtc ctcttcatgg ctgaggccta
     2941 ctgggacaag gaatgggagc ttcagcaaca ggggtttgat tactgctacg acaagcgact
     3001 ctatgaccgt cttgagcatg gccaggccga gagtgtacga ctgcatctct gtgccgatct
     3061 gccctaccag gagaaactgg ttcgcttcat cgagaaccac gacgaaccgc gcgccgctgc
     3121 gacgtttgcg gataagcagg cgcaggctgc ggcggtgacg ttcgccaccc tgcctggcgc
     3181 cagactgttt cacgaagggc aattcgaggg acgccgcgtg agactcccgg tgttcctgcg
     3241 ccgccgtccc gatgaaatca cggaccggag tcttcaggag ttctacaact ccctccttca
     3301 aacgctccgc agtgcagctg tcagggacgg ggagtggaca ctgtgcgaac ggaccggctg
     3361 gccggacaat gacagcttcc tgaatgtggt tgcctggtgc tggcaaaaag gggcagaccg
     3421 ccacttgatc gtcgtcaatc tttcaggggt tcgttcacag gcgcaggtca aattgccatg
     3481 gggtgatctc gccggccgtt cctggcgctt gaacgatccg gtgcagggga cggtctatga
     3541 tcgagcgggt gacgagcttc atgcatgggg cttgtatgta gacctggagc catggggcta
     3601 tcatgtgttc acggtgagca gcgggacctg agtcgcataa cgcggaggca aacatggttc
     3661 cttccaagag acttcagtgg cttggtttct tcttctgcgt gatgatcctc gcgggctgcg
     3721 cggaattagc tcaggatgcg gagagtctta tacactcgag cttacgaaga tctgacgtcg
     3781 tcggctttgt ggcaggcttc ggaacgacct ttgccgccct gccggatttg atcgcgatgc
     3841 tcaagcggag gtcgagtaaa ggcatgaatc cgcggatggc tgcgattatg ggcgcgttcc
     3901 aaatcctatg ggtctggtat gggttgttga tcgtttcacg acccgttgtc ctttggaata
     3961 ttgtcgcagt gacgacgaat tttatcagcg tggccgcata tttctacttc gcccgccgcg
     4021 agcactgaca ggcacgagct actgtctcct tcgctgtatc aaatcaacag gcgcgtccgg
     4081 ctgttgcgaa ccatgtggca ccatcggcca gtgcgacgac ggtcaggcct ttcttgggaa
     4141 gtgccatgag gaggaagcga taggcatgga tgtcctacgc gatatgggcg tgagaagaat
     4201 agccatcagc ggactgttcc tggtggcagt gttcgctctt gatctggtca ctcccttggg
     4261 gataccagtc tggctgttgt atggcgtgcc gtttctgttc ctgcagacgt attcgccacg
     4321 gtatcatgtt cacggcctcg cgatcgtctg cactttcctg atttagatcg gctatttcct
     4381 ttccccgcaa ggtcacccgc aactaacaaa cgagcgtttc ggaacaacca ttatcgtctg
     4441 ggttatcgtg ttgctgttcg ctcgtcgaac accttcacca caagggcgat gatgccgaat
     4501 gtgcggaagg cgcgcgaccg ctctgttccg gtcgcctcca tacgtcacgc tcatcgcatg
     4561 aagtagaaga ggaccagcac gtagagagcg gacaccgcga gctggcaggc cgtgattggc
     4621 aggccgtacc gcaaccacgt gatgaacgtg accggtttcc cgttgcgcgc gcagatgcct
     4681 acgctgacga tgttggcaga ggcaccaatg agcgttgcgt tgccgccgag cgtcgccccg
     4741 aacatcatgg cgacaaacac gggaatgata gcaacgggcc agtcggtgaa ggaggctccg
     4801 agcgccgcct cgggcaccgc ctcgaccgcc accaggtagc ctttgaccat gaagatcgag
     4861 gctgccacta ccgggatatt cgccagcagg ctcgacagca gcccgatccc cgcgatcagc
     4921 gccaacgcag cgagccagaa ctccgtgccg aaccaatcgt acagtttgag cgagaagctc
     4981 tgcagcagcc cggttttcgt aatagcctgc accaggaaga agatgcagcc gaggaatacc
     5041 agcgtcttcc agtcgatatc gcggagcacg ttgtcgatcg gttcaatctt ggcaccatag
     5101 atgacgagca aggccagcga agcagagatg attgccactg ccggtggacc gatggggtcg
     5161 ggcaaatctt ccccgaacaa aaacaatccg atcatgatcg ccaataccgc aaatgccagc
     5221 agggcgaacc accgccgctg gaaccgtttc tcagtcactt gcgggggcag ctgcctctgc
     5281 aagcgccaca gatcgggcat gaggagcggc agaaggggaa tgagaaccaa cagcgaaaga
     5341 agtccgccaa ggctcacctt gtgcagatac tgcccgaagc tcatgccgat cgaactgccg
     5401 acgaggaatg tggccgggtc tccgacgagt gtcaggagtc cggctgaatt actgaccatg
     5461 gcggtaagca ccatgggtcc cacgaagtcc acttcaagcg ccgtcgcgac gcggatgatg
     5521 atcggtgcca ggaggatcac tgttgtcgcg ttggggagaa acgcgcacaa cggcgcgacc
     5581 agtgcgacca acaacagcag aaaccgcttg ccgctgcctt ttgtcgcgtg gagatagctt
     5641 gcgccgatac ggtcaaagat gcctgtactg gcgagggtgc gcgcgaccac catgccgccg
     5701 aacaagagcg acagtggccc tccggcggtc ctcatggctg cgatgacgtc gtcctcggcg
     5761 agaatgttaa acccgaacaa cacactcacg ccgagcaggg tggcgactgc catgtcgatg
     5821 acatcgaacg caatcgccag gatcactgca ccgaacactg ccatcgtgat atagatctgc
     5881 agttcgctca tgcgcggact cccgtcgctt aacaggctgc ggaaaaacta tgtgggtatg
     5941 ccagaacttc gctcgttcgc gcgtcagaga taacaagaac acaccttgca ggatgctcaa
     6001 aaaggccgtt cagcaaggcc gcagcgagcg aagaggcgag gaggcacata ccaagcttcg
     6061 cttggtccgc tcgcttcgat cacatgcgag cggatagtta ctttgcctca agcggccttc
     6121 tccgtacgtt gagcctctga gcgacccgag aacgaagctg gcggactttt tcagcatcct
     6181 gttaacggac aggcggggca tacatcagcc cgccatgcgt ccagagccga ttcaggcctc
     6241 gatcgaggtg gagcgggcta tggactccga ggttccgttc gaatatctcg ccgtagttac
     6301 cgacactctc cacagcgtga agcgcccagt cctggtctgt cccgagcatt gggccgaacc
     6361 cgccgtcagc tcccagcgcc cgtcgtgcgg ccgggtgccg cgtggtttcg cggatctcgc
     6421 gtatgttgtc gcgcgtaatg ccgttctcct cggcggcaat gagcccaaag aggacccagc
     6481 ggaccagcgt cagccactcg tcgtcgccgc ttcgcaccac cggcccgagc ggttctttcg
     6541 aaaggtgttc cggaaggatg tcgaaggtct gcccggccgg cgcgcgtaac cgcgcgccgg
     6601 cgagatcgga gatgtccgac gtataggcct tgcagcggcc ggagaataaa gcgtcggcca
     6661 cttcagtcgg ggagtcgatg acgaggggct tcaccctgag gccctgggca gtgaagtgat
     6721 cggtgaggtt gcgctcgtgc gtggtgccct tctcgacgca tatggttgcg ccatcgagct
     6781 gcttcactga ttttatgcct tcagccgccg gcaccatcaa ggcctgagca tcgtagaaca
     6841 aaatcccggg gaagcgcagt tttaggcttg cctcgcgact cagtgtccat gcggtggcgc
     6901 gtgccaggag gtcaatgacg ctggacttga gtgcggggaa ccgctccgag gctcgaagcg
     6961 ggacgaaggt tactttcttg ggatcgccaa agacagcagc ggccaccgcg cgacaaaaat
     7021 ccacttccat gccggaccac tgtcccgatg cgtcttttgt cgagaagccg ggctttgcct
     7081 gattcactcc gcagtgtaac gttcctcggg ccttgatctg tccaagcgtg tcgcccgctg
     7141 cggcgggcga cacagcccac acgacagcga acaggactgc ggcgggaaac aggatcctgg
     7201 caaccgcgct aactggcatg ctggagccga gttgttttca catcttctcc gtacaacctg
     7261 atcaatgcgg tcgcgagcat cccgctgcga aaccgaccct ggaaaaacgc ggccattgta
     7321 acgatgagcg ggttgaaagt caccttgggg gaaaggtcag tgtcgcgaca ttgctgattg
     7381 agccgtattg agcgtggagc aaggaagggt gaatctcgtt gtccgcaacg agcgactgcg
     7441 atcccggtag tcgaccgaat ctcgaagaag tccccaccgg ttgttgcatt agtcaccaga
     7501 ctatccgaaa gaaattgtcc cgaaacgccc caataaacag tctttgcttc gagatcgact
     7561 cccggacgga tgcgaaagct cgttgttttt cacataacgg tttcgatttg tttcagaggg
     7621 gtataacggc acaggctgtg caaccatcga tacagatcct cgcggccagc actcgggctc
     7681 tggtcgcggt agtgtgattg tcggctatga cagatgctgg ccggctgacg ttctgggtat
     7741 gttcgactcc ggttagccgg gcgcttggtc gatcgaacat ctaaggagga agctcatgaa
     7801 cgcgcaaatg ttgggggtca tcatcctatg cgggacaatg ctggcgggat gcgttacgaa
     7861 aagcacgcat gaggcgaccc tgacggagct cgaccaggcg cagaaggcct cttcgaagat
     7921 ggcggcagac ttcgacagtt ttaagaaaca gtcggcagcc cagatcgcgg cgttggaagc
     7981 ggacaaaagc caactctcca acgatctact taactcgcaa acagcctctg cgaagctcca
     8041 acgagagctc gataaagcca gaagcagtct ggatacgacc ctggccagcc ggaatgacat
     8101 ggaacaagat gtacgaaaga tccgggccga atcatcgggg atggagcggt tgaacgggga
     8161 gcttcgccgg gaacgcgaac agctgcaaga caagctggat gctctccaaa agaattacga
     8221 tgccaccaag caggatctcg aaacgaagtt gaaggccgaa caggatactg tcgcggcgct
     8281 ccaaaacgac aagcaactcc tgatcgcgag agccacgggc gcgacggatg aactggccaa
     8341 gttgcagaaa cggatgggcg aactcgaaac cgacgcctct cgcgccaagg atctcgagca
     8401 acgtctctcg caacgcgaca tcgagcttgg gaaattgact caaggtggag agaccctggc
     8461 ggcggaaaag gaacgactgg ccgctgagaa ggaacgcctc gagaaggagc gggccgccaa
     8521 gcaagccgag attgaccggc tgaccaagac tcagcaggag ttaacgaagt cactgcaagc
     8581 tgaaatcgaa cgtggtgata tcacgatcaa gcaggtgcgc gatcggctga cgatcaatat
     8641 ggtcgaaaag gtgctgttca attccggtca ggctcaggtg aagccggccg gactgaaagt
     8701 gctcacgcaa gttagcgatg tcctcaagaa tgtgaccgat aaacaaatcc gcatcgaggg
     8761 acataccgac aacgtgccga tcggtgtgaa actccaggag aagtttccca cgaattggga
     8821 gctgtccacc gcgcgtgcca cgaatgtcgt gcgattcttc atcgatcggg gtggcgtcaa
     8881 tcgggagaga cttgaggctg tcggctttgc ggataccagg cctgtggccg gtaacgagac
     8941 ggaggccggc cggacagata accggcggat cgagatcgtg ctgtatccga aagatctctc
     9001 cgagatcgtc ggagaggcca aaccatagga aggggtaaaa attgtacctg agtggacgac
     9061 gggaaggctg agcatgcaga caacaggaaa tagtgctccg cttggcgcga cctactgtca
     9121 tggcggcgtc aacttcagcc tgtactcgcg cgaggcaacc ggtgttgagt tgctgttctt
     9181 tgatcggctg gacgatgcca agccggcacg tgtcatcggc gtcgatcccg tatctaaccg
     9241 cacctatcac tactggcatg tgtttgtgcc gggggttcgg cctggacaga tttacgggta
     9301 tcgtgctcat ggccccttcg atccagcgaa cggattgcgg tttgatccca ccaaggtgtt
     9361 gctcgatcct tacggtcgtg gcgtcgtggt accaaagaaa tacagtcgtg acgcggcgag
     9421 tgaaggaggg gagaacactg cgacggcgat gaagagtgtg gtggtggacg catcgacgta
     9481 tgattgggaa ggcgatgtga cgctcagacg gccctcggcg cggaccatcg tctatgaaat
     9541 gcacgtgcgc ggcttcaccc gtcatcccag tgccggcgtc gcagagaaca tgcgcggtac
     9601 gtatgccggt ttgattgaaa agataccgta cctccaagaa ctgggaatca cggcggttga
     9661 actgctgccg gtgttccatt tcgattctca ggactgcccg ccggggagaa tcaactactg
     9721 gggctatgcg cccatttcgt tcttcgcgcc tcacccggcc tatagttccc gacaggacgc
     9781 gctcggccct gtggacgagt ttcgcgatat ggttaaggcg ctgcatcgca ccgggatcga
     9841 ggtcattctc gacgtggtgt tcaaccacac ggcggaaggc gatcagcatg ggccgacgct
     9901 gagtttccgc ggcttggaca acaacacgta ctacatcctc gagcaggacc gttcccgcta
     9961 tgccaactat accggcacgg gaaacacgct gaacgccaac catccgatcg tccggcgcat
    10021 gattgtggac agtctccgct attgggtgga ggtgatgcac gtggacggat tccggttcga
    10081 tctcgcgtct attctcgccc gcgatgtgtc gggttgcgtg atgccgagcc cgcccgtctt
    10141 gtgggacatc gagtcggatc cggcccttgc gggcacgaag ctgattgccg aggcttggga
    10201 tgcggccggg ctctaccagg tcggcagctt cattggggac agttggaagg aatggaacgg
    10261 ccggttccgg gacgatgccc gaagtttctt ccgcggggaa gagggatcgc tggcgcgttt
    10321 tgccgaccgc atgatcggca gcccggagat ctacggacac gagcaacgcg aacctgagca
    10381 gagcgtcaac ttcgtaacct gtcacgacgg ctttacactg aacgacctcg tctcctacaa
    10441 cgagaagcac aacgacgcca acggcgaaga caaccgggac ggtgccaacg acaatcgaag
    10501 ctggaactgc ggagtcgaag gtcccacgaa cgatcctgac gtggagaagt tgcgcaaccg
    10561 acaggtgaag aatttcctca ccgcgacgct tctctccatt gggatgccca tgattctgat
    10621 gggcgacgag gtacggcgca cgcaacgtgg caacaacaac gcctactgcc acgacaacga
    10681 attgaattgg ttcgactgga ggctggtcgg taaacacgcc gatgtccacc gattcgtcac
    10741 gttgctgaac gaacgacgga tgttgcgggc tgtggagcat gagcgccaac gtctgagcct
    10801 ggtccaactg attcagcagg cgaacaaagc atggcatggc gtcaaactcg ggcagccgga
    10861 ctggagcccg cattcacaca gcatcgcgtt cagcgtggag atgagaaagg agccgcttct
    10921 tgtctatatg atcctgaacg cgtactggga gccgctcgat ttcgagctgc ctccggtgca
    10981 aaacgacagg gcaatcttat ggaagcgatg gatcgatacg gcccttgatt caccacacga
    11041 catcgtgccc tggcagaccg cgccgtcgat tcccggttat tcttatcggg ccgaatcgcg
    11101 ctctgtggtg gtgctgttcg cgaacctgac ggggtgagtg aggattgaaa cgtgaggcgg
    11161 gaggcgggag gagtgaatag ggaggagtca ggagatttct gatggcggcc ctatcgctct
    11221 aatctcctta ctcctcactg cttacttttc actgcaagga cgagggagga gtgagaagta
    11281 aggagtaagg agggcgggaa atgccgcgct gtacgccgcg ggaagatgca gctatactcg
    11341 gccggcgaaa atgaaactga aataggcaag acatctctag aggagggaca acacgatggc
    11401 gaaagacgac gggcaagagg aaaacggcaa gggaaaactg aagcgcaaag aatacgagaa
    11461 ggaattgcgg cggcttcaag ccgagctttg ccatcttcag gcctgggtga aacacaaggg
    11521 cctgcggatc atggtcgtgt tcgagggccg cgacggggcc gggaagggcg gcacgattcg
    11581 cgccatcacg gagcgcgtca gtccccgcat cttccgtctc gtggcgctcc cggcacccaa
    11641 cgaccgcgag aagacccaaa tgtacctgca gcggtacatg gcgcacttcc cggcggcagg
    11701 ggaaatcgtc atctgggacc gcagctggta caaccgcgcc ggagtcgaac atgtcatggg
    11761 cttctgcacc aaggaacagt acgaaggctt cctcgagcac gcctcaatta ttgaggggta
    11821 tatcacgagg gccgggatca tcctgatcaa gtactggctc gaggtgagca acaaggagca
    11881 ggaacggcgg tttgaggctc gcgtcgaaga tccgttgcgc cagtggaagc tcagtccgat
    11941 ggacctgccg tcacgggaga aatggtacga gtactcccgc gcacgcgacc ggatgctgga
    12001 ggcaaccgat tcgaagcacg caccgtggca tatcgttctc tcggataaca agaaaagcgc
    12061 gcgcctcaac tgcatcaccc atctgttgtc ggtgattccg tacaaaaagc tgccgcggga
    12121 aaagatcaag atccccaagc gatccaacaa gtacaaatac gacgatcagg cgtcgttgaa
    12181 ggggagacgg tttatcccgg aggtctactg acgccggcct atggcgatat tcctgggata
    12241 ttccgtgcca ctctgaccgg tgatactacg aaggacctca gtgcaggcag ctgcactgag
    12301 gtttggtgac gaatccctcc ggtcgatgag gagagaaatt ctgggttagg agtcttcggc
    12361 caagctcacc gggaagctag agacgaccat acgtcatgcc aattgtcacg cttcaccagc
    12421 gatgggagca gattctcgag aaccgctgtt tcttcctgtt cctggcgatg ctcatcctac
    12481 ttgtctcgat gccgttcctg ggcgagaccg tccacggtcg tctcataatc ggtgtcatca
    12541 acatcaccat gttgatgacc gcggtcgcgg cggtcggacg ctccaggttc tcattactgt
    12601 tctcgttcat tctggctgtg cccacgctta cctttcaggt tctggcgtta cagtctggcg
    12661 atgcggggca cttcgcgttg tcctggagct gtaatgcggt gttttatagc cttaccctca
    12721 ccaggctcct gcattacgtc ctgcgcaaag acactattac cgccgacaag ctctacggtg
    12781 ccatcgccgc gtacatcctc attgccatac tgtggggatt cctgtatggc gtgctgcagt
    12841 atttctatcc cggcgcctat gcgctgggag ggacgccaaa ggttctcaac atgggggatc
    12901 tgatctattt cagcttcact gcgctatcga ctgctggttt cggcgacatt actccgctgt
    12961 tgatccagtc ccgcttcctg acaatcctag agatgataac cggcgtgatg tacgtggcca
    13021 ttctgatcgc gcgcctgacc ggcgtttatc cgatcgtagg gacgaaaccc cctgatacag
    13081 gtgggcaagc ggggtaggcc gcgagtgggt gccgacatag gcctgcttgt gttctccggc
    13141 gggccggcgg atgtggcgat cgaggtgagg cggcaggcgc cggccttatc tcctgttgac
    13201 ccgcgaaggg tttcagcctc gcaggtctgt ggagtcgcca tgcttcacgc gcgtggaatt
    13261 cttgcgggtt acggttgttt tggcgcgttc tgtaacagtt cgctgcgcgc tttttccttc
    13321 tgcgcccgct gttcctcgat cagcgtgtag gccacgtagt acttataaat attggcgaca
    13381 tagatcaccg tctcctcccc gatgcgctgc gcggcgatga tttcaacgtt gttgaaccag
    13441 acgtggggat cgaatccgcg cttttccgcc tctttctgga gcttcagaac cttacctggt
    13501 ccggcattgt acgctgcaaa cgcgaacagg gttttgttgc gcatgtccat cggctgatcc
    13561 gcgaaaaact ggttgcgcat caggctgatg tatttgacgc cggcgtggat gttcggttca
    13621 acctgggtga tgtcgcccgt gtccatctcc ttgccggtgg cgggcatgag ctgcatcacg
    13681 ccgatcgcgc cggcgtcgct tctcgcgttc tgatcgagca gcgactcctg gtaaccctgt
    13741 gccatcatca acaaataatc catgtcatac cgggcgccgt acttgcgaaa gaattcaacc
    13801 gtctgttcga acttcttgat ttcggatgct gacgaagcag gtttcacgaa ccgtgtactg
    13861 gcggcgtatt tcttgatcag ggattttcca aagtcggatt tcatgccgta gttcttcgcg
    13921 aatgtcgcga tctccgcttt cagcttcgga ctgtctttgc ggaacatcca ggcgatgtcg
    13981 cctccctcgt tgatcaccag gtcttcccgg ggcttgagct tcttgaagac tttcgcccac
    14041 aacagcccct gataccggtt gacgacgacg atgctgacaa gtccggcatt caccatttcc
    14101 aggagatcat catcctgcag atcctccggc gcggctttca ggatgaccgg cggcttctgc
    14161 tctttcgcga agcgctcgtt gagacgttcg agatgagtcc agtagctcga cgacttgcgg
    14221 gcgaacactt ccttccctga aagatcttcg atcgaggcca gctcgggaga cgccggtccc
    14281 gtcaccacga tttgcttgac gccacggaag aacggctctc cgaaatcgac ctgttccagg
    14341 cgttccggtg tcacggtcaa gatgccggct gcgatgtcgc cgcggccttc caacaggcct
    14401 gagatcagtt ggtcgtgtct tgtcgggata atagccacaa cgatacggat atgcttgcct
    14461 tgtgtcttgt aattgttatt gagctcgttt tcaaaggcct tgaggagttc gtattctgcg
    14521 ccgatctgtc gggcgccgtt gagccaatac gccgtgcggg atggcgagac cagcgcgcga
    14581 atcacgcggc gcttgaccat cccgtcgagg tctcccgtcc acggttttaa ggcaggagcc
    14641 tgtgtcagct catgcttctt cgacgtttgt ttttcctctg cgaaggcgga cagggagagc
    14701 aagagacaga tgcagacgac gcctatggag acgagcttca cggagaatcc tctctctcag
    14761 tctggtcgta aggctgtgca ggattactca aacggcgggg aggttaacat tcgagatttt
    14821 aggggtcaag ttcgtgcgag gaaggagaga cgttgtgcct tgagcgggtg tatcgctcgt
    14881 cgttcggtcc gatcgatcaa tgtcattggg gtgtgaaggg gagctgcagt ccgaccaagg
    14941 atggccggac tgcgctcagg ttgaaggagg tcttaccgcg ctgctccatc aagggttcgg
    15001 ttcgacgcag ttcgtccccc cgcgaaattg gcatagccgg tagcgggtct tctcggccca
    15061 aaaacggtaa gcctcctcca cgtcatgcca agaactggtc atgccactca ggctcttggt
    15121 gccgcctctc cgatctaccg ccgcaagaag gagggttcca gtctcaccat ccgtcacctt
    15181 caattcagca ctcgcactgc ctgtgaaccc tgatacgccg gtcgccatgc cctttgcccc
    15241 tgtgagcagg cgggtctgcg ggataatgct tgtgaccgta tccagtaccg cgttcgattc
    15301 tcccgcttcc gtaatggccg cttgcatatg aatcacgtcc ggaccacctt gacttgtcat
    15361 ttcgtaatct tttctgagtt gttcgtcgag cttcgaccag agtagcgccg ccaggcgctg
    15421 ccggtcttcc accgggacat ccttgagctg agagtccttc tgactcatcc aaattgtcac
    15481 ggggtcaagt tgaattttct tgtacttacg ccaatccgcg gcagggttct tgtagaccca
    15541 caaggctgta tcttgagccc cttccttgaa cgtactgcgt tcaccctcct tcaatatgga
    15601 atagtctccg agaaatccgg acttttccac cttactggct tcttgtgttg cggcacagcc
    15661 tccaatccca acgagcaagc caatcagcaa cacactggcg atacgacgca tatttgcctc
    15721 cctgtttgta tagatgtcgg ttccagcctc cgggctgtgt actgtagcca ggtgctcaaa
    15781 aattagcaaa ttgtgacgca gacattgtga gattcttaca cgacgcaatg tcaccgttcc
    15841 agatccgatc ccgcttgact cgccactgag cactcgatac agtgcgaccg gaactgatct
    15901 tagcccgcat tattcatgag cgcttcttct gcaagaaaaa tggagatcaa acaaataata
    15961 cactgaggta tctttcctct cgcgccaaga tcaatcgcat ggcgctcgac ctcgctagcg
    16021 cgggggcgac acgcctgctt gcggctttgc ggtataccga cgagagggct cgcggctcag
    16081 gcccttaaca tactgttcaa gtatgcttcg actcctcgcc gctccacgcg ctcgtctcgc
    16141 aacgcgactg cgcgatctcg cgacgaaccg tcatgaataa tgcgggctag gtagaacgac
    16201 attcatcgag ggctatgatg tcttctccag attcagatcc aacccacgca tccggattct
    16261 ctactcccgt cggcacacgc cgaaaattct ttcaatgggt taccagggcg gcggcaggat
    16321 tcatcggcct ggggctcgct gttccattac tcggttacgt gatctctcct gcactcaagc
    16381 ggcgtgataa accatgggtg gaggttggtg gggtggacca actgacggcc ggcgagccca
    16441 agcaactgga ctatgtcgcg accattcatg atggttatct ggaactcacg acccataagg
    16501 cagtctgggc ggtcaagcaa tcagacgggc aggtgacagt gttttctccc atgtgcactc
    16561 atttaggatg tggctatcac tgggatgtca ccgatcggaa gttcaaatgt ccctgccatg
    16621 gcagtgtcta cgatgtg
//
LOCUS       DATIFR010000151        15087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2204464, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000151 DATIFR010000000
VERSION     DATIFR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204464"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VLD60_09160"
     CDS             <1..1629
                     /locus_tag="VLD60_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSB45169.1"
                     /translation="WEKHVLAPALKRAPEADRPVTTGSGAPLEPLYLPQPSSEATYLE
                     RVGFPGQYPFTRGIQATMYRGRLWTMRQYAGFGTTEETNRRYQYLLQQGSTGLSVAFD
                     LPTQIGYDSDDPLVSGEVGRVGVAVSTLRDMETLFRDIPVGRVSTSMTINSTAAILLA
                     MYLVIAEQQGVPAQLLGGTIQNDMLKEYIARGTYRFPPQPSLRLTRDLFVYCTKFLPK
                     WNSISVSGFHMREAGATAAQEIGFTLANAICYLDIAVQAGLDVDAFAPRLTFFFTAQS
                     DLFEEIAKFRAARRLYARVLKDRFHAKDPKSMMLRFHTQTSGVALTAQQPENNVVRVA
                     IQALAAVLGGTQSLHTNCRDEALALPTEESVRLALRTQQVLAYESGAANTIDPLGGSY
                     LIESLTDRLEAEAEQYIRDIEQSGGMVQAIEQGYVQRQIEQAAYEYGQSLETGARVIV
                     GVNRFREEHEPEPELFLVPEDTEQKQIEEIRMVKRSRSEQKVQASLDAVERTAQTDDN
                     LMPAILEAVRAYATMGEICQRLEHVFGEYEPILS"
     gene            1657..2436
                     /gene="scpB"
                     /locus_tag="VLD60_09165"
     CDS             1657..2436
                     /gene="scpB"
                     /locus_tag="VLD60_09165"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HSB45170.1"
                     /translation="MSYVITTIEDSIGTIMLNHGQKRNALSQPLIADILEALGAFRQQ
                     NLRVIILRAQEGATVWSAGHDVSELPQGHRDPLGWDDPLRRLIREVENYPAPIIAMIE
                     GSVWGGACEVAFACDLVIAADDTTFAITPAKLGVPYNLSGLLTFMSTISLHIVKEMAF
                     TGSPMKAERAERLGIVNHIVSRQELQRHTYEIAKQIVRNSPLSIAVMKEELNLLANAH
                     DLSPLMFERIQSLRRVVYNSQDYQEGIRAFLEKRPPTFTGK"
     gene            2593..2886
                     /locus_tag="VLD60_09170"
     CDS             2593..2886
                     /locus_tag="VLD60_09170"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSB45171.1"
                     /translation="MAIYIVLVNFTEQGVRNIKDTIKRGDAIRDMAKKFGVTAKEYYW
                     TLGSYDVIDIFDAPDDASMAAFLAAFGAMGNARTQTLRAFSQDEMKGILAKLG"
     gene            4112..5953
                     /locus_tag="VLD60_09175"
     CDS             4112..5953
                     /locus_tag="VLD60_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSB45172.1"
                     /translation="MAYQPIENYGIIGNMRTAALVGLNGSIDWFCVPSFDSPSVFAAI
                     LDDQKGGRFEIGPRDDDIRTKQFYWPETNVLVTRFLSPDGVGELEDFMPAGLPRESPW
                     QDQLIRRVRVTRGTLAFRMCCRPAFDYARLPHHATIVEHGAVFKSAVLSLGLATAVPL
                     SCDGESVQATFVLQEGEEAIFVLRPLKPGEECRQSSSNTETQEIFEHTVHYWHRWLSK
                     CTYFGRWREIVYRSALTLKLMTFEPTGAIIASPTCSLPESIGGGRNWDYRYTWIRDAA
                     FTLYGLLRIGFTEEAQAYMKWLEARAGEVKAAGTLQIVYGIDGRHNLTEQTLDHLDGY
                     RGSRPVRIGNGAYAQLQLDIFGELLDSLYLYNKHVMPISYDVWLRLRRGLDWLCDNWE
                     QPDEGIWEVRGGRHHFVYSKLMCWVAMDRGIRLADKRSFPADRDRWQRVRDQIYEDII
                     SKGWSQKRQAFVQHYDGDSLDASNLIMPMVFFMAPSDPRMLSTLHAINRSPKEGGLVS
                     DGLVYRYDSETGKDGLKGREGTFTMCSFWLVEALTRAGRVDCAKLTEARLLFERMLGH
                     ANHLGLYAEEIGGSGEALGNFPQAFTHFALISAAFNLDRALDGKPRS"
     gene            complement(6462..6896)
                     /locus_tag="VLD60_09180"
     CDS             complement(6462..6896)
                     /locus_tag="VLD60_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45173.1"
                     /translation="MTEYKSDLLFGALTGNGRVLVVDDEPSVRTVVRMILEKAGYDVL
                     EAEDGEKAIEAINTGENRLVLDAVICDIRMPKINGVQAINYFQTEYPHVPLIVLTAYP
                     STNMAVSFMGHGVVDYLIKPVEAENLRAAVEKAVERRQVTRV"
     gene            complement(7704..8009)
                     /locus_tag="VLD60_09185"
     CDS             complement(7704..8009)
                     /locus_tag="VLD60_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45174.1"
                     /translation="MKELEVKAQTVFTGRTNPITASNKPVMCIVDGCLSKASKSCEIT
                     VTSIEPIPATETHLVPLCDIHSQAGDTLKWAWRRAADAAASSRLKLDAVFALDTKED"
     gene            complement(8225..8770)
                     /locus_tag="VLD60_09190"
     CDS             complement(8225..8770)
                     /locus_tag="VLD60_09190"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HSB45175.1"
                     /translation="MNRVSLCREGNVMTAAFLPERIERAILLLRRHRIILDRDLAAMY
                     GVSTRDLNKAVSRNLDRFPDDFMLQLTRSEFNDLKFQFGTSSWGGTRKLPRAFTEQGI
                     AMLSSILRSKRAIHVNIAIMRAFVKLREFAASHKEMAVKLEQLERRVGGHDGQIRSLF
                     EAIRQLMEPPTQKSRRIGFKT"
     gene            complement(9021..9530)
                     /locus_tag="VLD60_09195"
     CDS             complement(9021..9530)
                     /locus_tag="VLD60_09195"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HSB45176.1"
                     /translation="MNTPAPAEGIEQTIRIIRGHRVLLDADLAGLYGVQTKVLNQAVK
                     RNRTRFPLDFMFQLTVEETTSLRSQFVTLKGRRGQHRKYRPFVFTEQGVAMLSSVLHS
                     ERAIQVNIAIMRAFVRLRGMISSNKILARRLAELEKKYDAQFRVVFEAIRELMAEPES
                     KSRRIGFKT"
     gene            complement(9782..10336)
                     /locus_tag="VLD60_09200"
     CDS             complement(9782..10336)
                     /locus_tag="VLD60_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45177.1"
                     /translation="MASGTNLVPTVLLPVLLAGLSLAGCVKHVPKVAIPEKDASPPKL
                     IWEVYNLQTKETKGIEQDGQSFVVAPGDLYVVTLAVEDLESGVKEVSLTGNADYKCEQ
                     GGKVEEKKYQLEPQTQKASPDLENKAPVTASLTSSVDQSKHGCTEHQRFGGGTISLTG
                     KAQNFVNGGGTKTLRINLQPRPSP"
     gene            complement(10394..10897)
                     /locus_tag="VLD60_09205"
     CDS             complement(10394..10897)
                     /locus_tag="VLD60_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45178.1"
                     /translation="MRNARASAWEHCCVSLTVVGFFCAGCALSERPVQMYEGALLPKE
                     QVGIVRSACKTGPGLTIMIVRLDDKETSNVCADFAVLPGDHNLELSAKRLAPKIDTPM
                     IRSASVLGAPPSPMGATPDDESSVIWASSSPLRITCTVQAGQEVTIVGNAGAGPDWEA
                     RCQERAR"
     gene            11545..11910
                     /locus_tag="VLD60_09210"
     CDS             11545..11910
                     /locus_tag="VLD60_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45179.1"
                     /translation="MSQEPTTEHLRIAELSETSTSSVDKLQRHILKKAAQLGAHAVVF
                     SLPSTHTEQRVDYQPVYSPWGYYAPYYYGPGPYGYWGPWGYSYGPWGPGWGYQQYVPV
                     WYFVRVTTLKGLAIRYSGS"
     gene            11951..12427
                     /locus_tag="VLD60_09215"
     CDS             11951..12427
                     /locus_tag="VLD60_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45180.1"
                     /translation="MNVYRPLLIGLGLVAAIGCGGPTVEREGGRSGDPVVEMLHRGIL
                     ELNGNIDELNHHIADLQQMPPVLDPRVQELRALDLSGWQLHLQQWILQREHLIFSVNQ
                     IQRVQANPREKLAIGTQWTNRQQHFIKSMDDLRTQRQTLERKRFEVESQVLGQYFK"
     gene            complement(12967..13527)
                     /locus_tag="VLD60_09220"
     CDS             complement(12967..13527)
                     /locus_tag="VLD60_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB45181.1"
                     /translation="MRNVMMMGLITVLGVGILWSSEAADQHDQMHGYGLGRPATDLEI
                     QQWNIDVAPTGEGLPLGRGTAKQGAAIFATKCASCHGPTGQEGPMDRLVGGAGTLGSQ
                     KPVKTIGSYWPYATTLFDYVYRAMPFPAPQSLSSDEVYSVVAWLLYQNGIIAEDSVLD
                     AHSLAGIRMPNHNGFVPDPRPDVPRN"
     gene            complement(13520..14794)
                     /gene="soxC"
                     /locus_tag="VLD60_09225"
     CDS             complement(13520..14794)
                     /gene="soxC"
                     /locus_tag="VLD60_09225"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HSB45182.1"
                     /translation="MMNSIQKEKRDEPRDEADTEKTALLNRRSLLAGTAGMMSAIVTH
                     SVTRFAEAAMPVTPDDPTRVPGGAPTAYGQRSTFEQSVRLPRSWWGSLTPLQDSHGIL
                     TPSALHFERHHNGVPVIHPERHRLMIHGLVAQPWAFTMDDLKRFPATSRLAFIECSGN
                     SASEWKAPTGQTVQQTHGLTSTSEWTGVALKTVLQEVGVKPDASWMLAEGSDAAAMAR
                     SLPLAPLIEEALLCYAQNGEPLRPEQGYPLRLLVPGWEGNTCIKWLRRLKFGTAPFMT
                     REETSHYTDLMPDGTARQFTMVMEAKSVITSPSGGQRVQPGFIDIRGLAWSGRGRIAR
                     VEVSTDGGRSWQQAVLQDPILPKCHTRFCLPWRWDGKDVILQSRCLDETGYVQPSRTA
                     LVAVRGLNSVYHYNAIQSWKIAADGEVSNVHA"
BASE COUNT         3263 a         4188 c         4281 g         3355 t
ORIGIN      
        1 tgggagaaac acgtcctcgc ccctgccttg aaacgtgcgc cggaagccga ccgtcccgtc
       61 acgacgggat caggcgcgcc cttggagcct ctctatctgc ctcagccatc ttccgaggcc
      121 acgtatctcg aacgagtcgg ctttccggga cagtatccat ttacccgcgg cattcaggcg
      181 accatgtacc gggggcggct atggacgatg cgccaatatg ccggtttcgg aacgaccgag
      241 gaaaccaatc gacgatacca gtatttgctg cagcaaggct cgaccggtct gtccgttgct
      301 tttgatctgc ccacgcaaat cggttacgac agcgatgatc ctctggtcag cggggaagtg
      361 gggagagtcg gggtcgcggt ctcgaccttg cgcgacatgg agacattatt ccgtgacatc
      421 cccgttggac gcgtatcaac cagcatgacg atcaattcga ccgccgccat cctcctggcc
      481 atgtatctcg tcatcgccga acagcagggc gtgcccgctc agctcctggg agggacgata
      541 cagaacgata tgttgaagga atatattgcc cgtggcacct atcggtttcc tcctcagccc
      601 agccttcgcc tcaccaggga tctgtttgtc tactgcacga aattcttgcc caagtggaac
      661 agcatttccg tcagcggctt tcatatgcgt gaagccgggg caacggccgc ccaggagatc
      721 ggcttcacgt tggccaacgc catctgttac ctcgacattg ccgtccaggc gggactcgac
      781 gtcgatgcct ttgcaccgcg cctcacgttc tttttcaccg cacagtccga tctctttgaa
      841 gagatcgcga agttccgggc tgcgcgccgc ctctatgctc gcgtattgaa agaccgtttt
      901 catgccaaag atcccaaaag catgatgctg cggtttcata cgcagacgag cggcgtggcg
      961 ctcactgccc agcagccgga gaacaatgtc gttcgggtgg ccatccaggc tctggccgcc
     1021 gtattgggag ggacgcaatc gctccatacc aattgccggg atgaagctct ggccttgccc
     1081 actgaggaga gtgtccgcct tgcactccgc actcagcagg tgctggcgta cgagtccggg
     1141 gccgccaata ccatcgatcc gctcggcgga agttatctga tcgagagcct tactgaccga
     1201 ttggaggccg aagccgaaca atacattcgt gacattgaac agtccggcgg catggtccag
     1261 gcgattgaac agggctacgt ccaacgccaa atcgaacagg ctgcgtatga gtacggacag
     1321 tccctcgaaa ccggcgcccg ggttatcgtg ggagtcaaca ggttccggga agagcacgag
     1381 cctgaaccgg agcttttcct cgtgccggaa gacacagaac aaaagcaaat tgaagagatc
     1441 agaatggtca agaggagccg tagcgaacag aaggtacagg cctcgctcga cgccgttgag
     1501 cgaacggctc aaacggacga caatctcatg ccggccatac ttgaggcggt ccgggcttac
     1561 gcgaccatgg gtgagatctg tcagaggctc gaacatgtgt ttggagaata cgaaccgata
     1621 ctaagttgat tcggattcca ccgaggagag ggtgtcatgt catacgtgat cacgactatc
     1681 gaagattcga tcggaacgat catgctcaac catggtcaga agcggaacgc gctgagtcag
     1741 ccgctcatcg ccgatattct cgaggcgctc ggggcgtttc gacaacagaa tttacgggtc
     1801 attattcttc gcgcgcagga aggcgcgacg gtctggtccg ccgggcatga cgtgagtgag
     1861 cttccccaag gacaccgtga tccgctgggt tgggacgatc ccctgcgacg ccttatccgg
     1921 gaagtggaga attacccggc tccaattatt gcgatgattg aaggcagcgt gtggggcgga
     1981 gcctgcgagg tggccttcgc ctgcgatctc gtcattgcgg cagacgatac gacgtttgcc
     2041 attaccccgg cgaaactcgg tgtcccctac aatttatcgg ggttattgac cttcatgagc
     2101 acgattagcc tgcatatcgt caaagaaatg gcttttaccg ggtccccgat gaaagcggaa
     2161 cgagccgaac ggcttgggat tgtcaatcac atcgtttcgc ggcaggagtt acagcgccat
     2221 acctatgaaa tcgccaagca gattgtccgg aattctccct tgagcattgc cgtcatgaaa
     2281 gaagaattga acctcctcgc gaacgcacat gatttatctc cactgatgtt tgaacgcatc
     2341 cagtccttgc gacgggtggt ctacaacagc caagactatc aggaaggcat tcgcgcattt
     2401 ttagaaaagc ggcctcccac gtttaccgga aagtaaatac gagagtggat gagatgccag
     2461 gctaggtcat gctgtctcac ttttgcaagc aaggtgttcg aacaatgatc acatagcccc
     2521 gcgtcaatca aataccagtg gcatcacctg tagtcttttc agtgaactcg acccctgcga
     2581 aggaggttgg tcatggccat ctatatcgtt ctggtgaatt tcactgaaca gggcgttcgc
     2641 aacatcaagg acacaatcaa gcggggggat gccatacgag acatggccaa gaagttcggt
     2701 gtgacggcga aggagtatta ctggacgctc gggagctacg acgtcattga tattttcgac
     2761 gcacctgatg atgcgtccat ggcggcgttc ctggcagcct tcggcgccat gggcaacgct
     2821 cgcacacaga ccttgcgggc tttttctcaa gacgagatga aggggatcct cgccaagctg
     2881 ggataagggc gagcaactgc cgatccaggc cgggagtact tctgcgaaag gttgagttgt
     2941 ccccggagcc tgaggagcgg ggccgtccat agatagcgtg atatggaatg cggaccccgg
     3001 cccttctctg cgttctacct tgatcgtgcc gccatgggca tcgaccacgt ctttcatgag
     3061 cgtggttccg agtcccgtgc ctccgacctt tcagctgctc gcctggtacg agaagagact
     3121 gtcacgactt tccggtgtca tgcccctgcc agtgtcgata atcgacaata caatgtgctt
     3181 tcccgatttg tcccgccgcc cttgaaccgg accgatccgc ccggcggaac ctcgggaatc
     3241 gcattgttca ccaggttatc ggatgaacca gagaaacaaa cgagaccaga tgagcgttct
     3301 tatactggta acccgaaaat agcgcgtatt agttcagcgg gacgggcaca aacgttctga
     3361 gggttggcgg tgatgaggtc ggtcactgaa caaggctatg tcgcggaaaa cagatggact
     3421 gtgagagtgt atctgaaacg gcaaatgacc cactcctacg tccacactac ttccaagaaa
     3481 tccaatctcc aacccctgaa aacttcacgc tcatgaattg cgcctccgct gtccctcaat
     3541 ccgatgatga agggatagtc tcgtcgtagg agcagcggtc caggaaaggg ttttcaccat
     3601 cctcctccag cccgctcaga ttcacttagg ccaagttcag ccaaggattg gccaaaactc
     3661 gaactttggg tggagttggt tggaatgagc tggagtgagg cggagtcgag cacaacacga
     3721 atccattcgg ctccccgatc tggatccttt gcgaactctc cagtttgaga tttctcgacc
     3781 tgctcgacct gcagtttacg aacacgacgt cggttgaaac acaaggtcat agtatgctgc
     3841 gccatacacc agcaggtcca agcccatcaa ggggtttgtg gcctgctgtt gcttcccatt
     3901 tcctcctgtg ttatcgcctc actgtggctc ttgtacgatc ggaaccgtat ctggcatcgg
     3961 gacacggaaa cgaacggctt gttcactttt cagcagtaca aattgtcacc tttcacaatg
     4021 gatcccctat agcattagca ttatagtttt cgcattgccc cacttgttcg caaaggccga
     4081 aaaatcacac gggtgagaag gaggcccagc aatggcatat caacccattg aaaattacgg
     4141 catcatcggc aacatgcgga cggccgcgct cgtcggcctc aacggctcca ttgattggtt
     4201 ctgcgtcccg tcgttcgatt caccgagcgt gttcgccgcg atcctggacg accagaaggg
     4261 aggccggttc gagatcggtc cccgcgacga cgacatccgt acgaaacagt tctattggcc
     4321 ggaaacgaat gtgttggtga cccgcttcct ctcaccggac ggcgtcggcg agcttgaaga
     4381 tttcatgccg gcaggcttgc ctcgtgaatc gccctggcag gatcaattga ttcggcgtgt
     4441 cagagtcacg cgagggacac tggcctttcg gatgtgctgc cgtccggcgt ttgattatgc
     4501 acggctcccg catcacgcga cgatcgtaga acatggcgcc gtctttaagt cggccgtcct
     4561 gagcctgggg ctggcgacgg ccgtcccgct ctcttgcgac ggcgagagtg tccaggcgac
     4621 atttgtactt caggaaggcg aggaggccat cttcgtgctg cgtcccctga agcccggaga
     4681 ggagtgccgt cagtcctcct caaacaccga aacgcaggag atattcgaac acacggtcca
     4741 ttactggcat cggtggctgt cgaagtgcac ctacttcggt cggtggcgcg aaatcgtgta
     4801 ccgatcggcg ctgacgctca aactaatgac cttcgaaccg accggagcga ttatcgcctc
     4861 gccaacctgc agcctccccg agtcgatcgg cggcggacgg aattgggact atcgctacac
     4921 ctggattcgc gacgccgcct ttacgctgta cggcttattg agaatcggct tcacggagga
     4981 agcgcaggcc tacatgaaat ggctggaagc gcgcgccggt gaagtcaagg ccgctggcac
     5041 attgcagatc gtctacggaa tcgacggtcg ccacaacctc acggagcaga ctctcgatca
     5101 tctggacggc tatcgaggtt ccaggcccgt ccggatcggc aacggcgcgt atgcgcaatt
     5161 gcagctcgat attttcggtg agctgctcga ttcgctctac ctgtacaaca aacacgtcat
     5221 gcccatcagc tatgacgtct ggctccgtct ccggcgcggc ctggattggc tctgtgacaa
     5281 ttgggaacag ccggacgaag gcatctggga ggtgcgagga ggccggcatc attttgtcta
     5341 ttcgaaactg atgtgctggg tggcgatgga ccggggaatt cgcctggcgg ataaacggtc
     5401 gtttccggcc gatcgcgacc gatggcagcg ggtccgggac caaatttatg aagacatcat
     5461 atccaagggg tggagtcaga aacgccaggc ctttgtgcag cactacgacg gcgactcgct
     5521 ggacgcgtcg aatctcatca tgccaatggt attttttatg gctcccagcg atccgcgcat
     5581 gctgagcacg ctacacgcca tcaatcgctc accgaaagag ggaggattgg tttccgatgg
     5641 cctcgtgtat cgctacgaca gtgagaccgg gaaagatggg ctcaaaggac gagaaggcac
     5701 gtttaccatg tgttcgtttt ggctggtgga agcgctcacc cgggcaggac gagtcgattg
     5761 tgcgaaactg acggaagccc ggctgttgtt tgaacggatg ttgggacacg ccaatcatct
     5821 ggggctgtac gccgaagaga tagggggaag cggcgaagcg ctcgggaact ttccgcaggc
     5881 cttcacgcat tttgccttga tcagcgccgc gttcaacttg gatcgtgcct tggacggcaa
     5941 gccacgtagc tgaccagttg cacaaactca ccgtgactct tttcggattg gagccgtgac
     6001 agaggccgtg acaaacaaga gccctggaca cgcatgtgac ctgtgttaat ccttgtgcac
     6061 cctgaatgtt ggtcatggtc ttcagacggt ccaaaatgct tggtcggttc cgggttcctg
     6121 gacgtcgatt gttcagggtt ttgtcccaat catcatgagg ggttaaaaag aattgtttgt
     6181 ggacagaatg cgagatatca cgatttagag aggcatttct ctgggaaccc tgaggtctgg
     6241 ccaggagttc gtcgttctgg aattgttctt gtcactttcg gacggcgaag agatcgatct
     6301 tcgtgatctg cttccggttc atccgtcgac ccaaagaccg gccctccctt atgcagaacc
     6361 gaggacaggg ggtagacgga gagaaccctt ccccccgacc tccggaacag agcgacggct
     6421 tctccgctcc gaggccaagg gggacatgaa gggacgatgc gtcagactcg agtaacctgt
     6481 cgtcgttcca ccgctttctc cacggcggcc ctcagattct cggcttcgac tggcttgatc
     6541 aaataatcca ccacgccatg ccccatgaag gaaactgcca tgtttgtact cgggtaggct
     6601 gtgagaacaa tgagcgggac atgggggtac tctgtctgaa aatagttgat ggcttggacg
     6661 ccattgatct tcggcatgcg gatgtcacag atcacagcat ccagtactaa tcgattctct
     6721 cctgtgttga tcgcttcgat ggctttctcc ccatcttccg cttccaggac gtcatacccg
     6781 gccttttcca gaatcatccg caccacggtc ctgacagagg gttcatcatc gaccacgagc
     6841 acgcgaccat ttccagttaa cgcgccaaac aataaatctg atttgtactc agtcatggca
     6901 tgctcctttt tgtgttgaac tccatttcac gacggtcgtc tgaaatcttg ccagcagaac
     6961 agatccacgc gctgcatgca caggttgaca tctgattgga ctcattgggc ccagagatcc
     7021 tccataacga acaacactac tgctatggtc aggccagctg agaagataag taagagatcc
     7081 attgtgaaac atcctttgta aactgtttgt aacgtactgc cacgatgtaa ccgcaacatc
     7141 tgtgccagat gaagattgac cgcgcgagct gaaatagttg aacagaagac ggcgattatt
     7201 cacggaacgc gattggttcc aggttccgac gatctccata cgttacgttg cttgacggca
     7261 ccgccgaagg catccaaacc gatgcagcat ggatgtattt cgattctctg agtggactgg
     7321 cgggaagacg tgaggcggga ggggtgaggg gtaagacgcg acgagcggga ctggcaggac
     7381 gagcgggaaa ggcgggacga gagcgaaaag cgggaggggt gaggcggaag gagtgaggag
     7441 taagggatga ggagttagaa gtgaggagga aagagatgat ggctgagagc tgatcgcgac
     7501 tagcgagatg ggcgtgagtc gtgcgatgtg gtgctacgtc gttctttgac tggggctaga
     7561 cagtacggac actgtgtggg cggctgctcg gtgacatttc ggcgtgagca ggagggatat
     7621 tcccggatcg tttgtccttt gggagggttt atcccggtgg gagtcggaga atttttcgag
     7681 tctgtcattc ccttctcccc tcttcaatct tctttggtat ctaacgcaaa aaccgcgtcg
     7741 agtttcagcc gtgaagaggc cgccgcatcg gcggctcgcc tccatgccca cttcaacgtg
     7801 tcaccggcct gggagtgaat gtcgcagagc ggaacgaggt gcgtctcggt ggccggtatc
     7861 ggctcaatgc ttgtgacggt gatctcgcag ctcttgctcg ccttggacag gcacccatcc
     7921 actatacaca tgacaggttt gttcgatgct gtgatcggat tcgtccgacc cgtgaacacc
     7981 gtttgagcct tcacttctaa ttctttcacg aggctccttt gcgacgtcag cctccgagag
     8041 gccaagacat tctttcaccg atcccacgcc catcctgcag cttccagctt tgacttcttc
     8101 ccttttctca cgactccttc tcgtggtcac actacctttc cgttcattca agaatgttat
     8161 gtcatatctg ccatcgtgtc gagccccgcc gcgagcaggg ggaggacaga ttgcctgact
     8221 tctctcaggt cttgaatccg atgcggcgag acttctgcgt ggggggctcc atgagttggc
     8281 ggatggcttc gaagagggag cgaatctgcc catcgtgccc gccaactctc cgctcaagtt
     8341 gctccaactt caccgccatc tctttatggc tcgccgcaaa ctcccgcagc ttcacaaatg
     8401 ctctcatgat tgcaatattc acgtgaattg cccgtttgct gcggagaata ctggataaca
     8461 tggcaatccc ttgttcagta aaggccctgg gtaacttcct tgtccctccc caacttgatg
     8521 ttccaaattg gaacttcaaa tcgttgaatt ccgatcgtgt gagctgaagc ataaaatcat
     8581 cgggaaatct gtcgagattc ctcgaaactg ctttattcaa gtccctggtt gagactccgt
     8641 acattgcagc caaatccctg tcgaggataa ttctgtgtct ccgtagcaag aggatggctc
     8701 tctcaattcg ttctggaagg aatgcggcgg tcatgacgtt cccctcgcgg cacagagata
     8761 ccctgttcat tgaacgaaat caacttcagc gtgccacaaa tggatgcatt aacaaatagt
     8821 tagaacgtaa gagtcaacgg gatcgtgagg ttctgagtca cctgggaagt tttgagttct
     8881 gggttgtcgg ccagcgagag gagcgggaag acgtgaggcg ggaggcggga ggcgaacgga
     8941 taagatgtgc ggagtgctga gacgagagtg ctgatggctg agagctgatg gctgatcgct
     9001 atgagcgcgg cgggttccgc tcaggtcttg aagccgatcc ggcgggattt cgactcaggc
     9061 tctgccatga gctcccggat cgcctcaaat acgactcgaa actgggcgtc gtatttcttc
     9121 tcaagctcag cgagccgccg ggccagaatc ttgttcgagc tgatcatccc ccgcagacga
     9181 acaaaggctc tcataatggc aatgttcacc tggatcgcac gctcgctatg taacacacta
     9241 gagagcatgg ccactccctg ctcggtaaac acaaacggtc gatatttccg gtgctgtcca
     9301 cgccttcctt ttaaggtcac aaattgtgac cttaaagacg ttgtctcctc taccgtcagt
     9361 tggaacatga aatccagagg aaatcgggtt ctattcctct tgaccgcttg gttcagcact
     9421 tttgtttgaa ccccatacag ccctgcaagg tcagcatcaa gcaatacccg atgtccgcga
     9481 atgatacgga tggtctgttc aattccttcg gccggcgccg gtgtattcat gcgcgcactt
     9541 caattttggc ggcctagaaa taccgtgtac attgagtgaa agcaagtgga cgagctcatg
     9601 aaatggaagc atttgcaagg agttgtgagt gggataatag aggatctgtt gagtttcaga
     9661 gatcgtgagg cggaaggcgc gacgagagcg aagagcggga ggcgtaagga gttaggagta
     9721 aggggtatgg tcggaagggg tgaggggtga ggggtgaggc gcgacggctg caactgctta
     9781 tttatggtga gggccggggc tgcaaattga tgcgcagggt tttcgttccc ccaccgttga
     9841 cgaaattctg cgcctttccg gttagcgaga tggtgccgcc gccgaaccgt tggtgttccg
     9901 tgcatccgtg cttgctctga tcgacgctgg aggtgagcga cgccgtgacc ggggctttgt
     9961 tttcgagatc tggcgaggcc ttctgcgttt gcggttcgag ctgatatttc ttctcctcga
    10021 cctttccgcc ctgctcacat ttatagtccg cgttgccggt gagggagacc tctttcacgc
    10081 cgctttcgag gtcttcgacg gcgagcgtca cgacatagag gtcgccgggg gccacgacga
    10141 aggactggcc gtcctgctcg atccccttcg tctccttcgt ttgcagattg tagacttccc
    10201 agatgagttt cggtggagag gcatccttct ccgggatcgc gactttcggc acatgcttca
    10261 cacagccggc tagggacagg cccgccagga gcaccggcag tagaacggtg ggtacgaggt
    10321 tcgttcccga cgccatcgta tgcgcctcct tgttgatctc actgaagcgg agtagaacat
    10381 ctgcctgcca tgcctaccgt gctcgctcct gacatcgggc ttcccagtca ggccctgccc
    10441 ctgcgttgcc gacgatggtg acttcctgtc ctgcctggac ggtacaggtg atgcgcagcg
    10501 gcgatgacga ggcccagatg accgatgact catcatccgg cgtcgcaccc atcggagacg
    10561 gaggcgcgcc taacactgac gccgacctga tcatgggcgt atcaattttc ggagccagtc
    10621 gcttggcgct gagctcaaga ttgtggtcac ccggcagcac ggcgaaatcc gcgcagacgt
    10681 tgctggtctc cttgtcgtca agccgcacga tcatgatcgt cagcccgggc cccgtcttgc
    10741 atgcactgcg cacgataccg acctgctctt tgggaagcaa cgcgccttca tacatctgga
    10801 ccgggcgctc actcagggca cagccggcac agaagaaccc caccacggtg agtgacacac
    10861 agcaatgttc ccatgctgac gccctggcgt ttcgcatggg ccttcctctc cgatcactcg
    10921 acgaagaaac ggtgtgcgta tccattgcgg tttcacgcag aggtggtgac gaagaatact
    10981 cgtttttcac agcgaaatca tctcagccgt ttggggaagt gctgagtgcg ggaagacggg
    11041 aggcgtaagg ggtgaggggg taaggcggga cgggcgtgaa aagcgggaca ggcaagaaga
    11101 gcgtgaggcg gaagggggtg acgcgcgata tcttgtgaat tgtgaccctg tagttcgtaa
    11161 cggcaagggg aagatcggcc ttgtaacccc acatctggag cgcaggtttg gccgaggacg
    11221 cattgccacc accttgactg cccgaggagt tcttacgaag acaggcgcgc catggtcgcc
    11281 gcaggcaatc agaaatattc ttggacggga aaacaccgca gcccacatcc tctcctcttt
    11341 gtttggactg acatctcaca ctctatcgct ccccgtagcg ggaacgtctc cggacgcagt
    11401 acgatagata gaatgagttc caaaagacgt ctaatcctca ttgctttgct tttgggctgg
    11461 gcatgcacgg gctgctccaa tgtgaatgtg ctccggttga cctccgagac atttcctcct
    11521 cgccctgtag gtgaagtcga gatcttgagc caagaaccga ccaccgaaca cctccggatt
    11581 gctgaactga gcgagacttc cacatcatcg gtggataaac tccaacggca tatcctcaaa
    11641 aaagctgctc aattaggcgc tcacgcggtg gtgttctccc tcccgtccac gcataccgaa
    11701 caacgagtgg actatcagcc agtctacagt ccatggggat actatgcgcc ctattactac
    11761 ggtccgggtc cctacggata ttggggaccg tggggctaca gctatggacc ttggggaccc
    11821 gggtggggtt atcagcaata cgtaccggta tggtacttcg tccgcgtcac taccctgaaa
    11881 ggcctcgcaa tcaggtactc agggtcttag agcttgaagc caacaaactg ttagatggga
    11941 gggatgatct gtgaatgtgt atcggccact attgatcggg ctgggcttgg tggccgccat
    12001 tggatgtggc ggaccgaccg tggagaggga ggggggacgg tcaggcgatc ccgtagtaga
    12061 gatgttacat cggggcatcc tcgagctgaa tggcaacatt gatgaactca accaccatat
    12121 tgcggacctg cagcagatgc ctccggtctt agatcccaga gtgcaagagt tgcgcgcgct
    12181 agacctctcg ggctggcaac tgcacctgca gcagtggatt ttgcagcgtg aacatctaat
    12241 tttttcggtg aaccagatac aacgagtaca agcgaacccg cgggagaaac tcgcgatcgg
    12301 gacgcagtgg accaatcgac aacagcactt catcaaatcg atggatgatc tccggactca
    12361 acggcagacg ctggaacgca aacgttttga ggtggaatca caggtgctgg ggcaatattt
    12421 caagtgagca aacggccgag taacctcacg ggggcaacgg tcgagctgat ccctcgatcg
    12481 gctggcgttc ctggcgttcg tgcctcacat cccctaacca ggaacccgag agggaccggt
    12541 atgtatgcaa cccgcgtgtt ggtgttggct taaaggagga tggaggagcg gcttgttgag
    12601 tcagtgcttg tgcaagccgg ggtctcttta atccctttaa aatgtttctc aagttctaaa
    12661 aggcggagca atcaccccct gcgtgtgcgg agcgccgaga aatcatcctt gccagctagt
    12721 gcgccaaatt gtatcgaacg gcactgagta attcgtgcat atctatgggc ttatgaagcc
    12781 ttcacacggg ggtcagtctc accccttttg aaaagccgcg aaacatcgcg tcaacactgg
    12841 cgaataacgc acatggggaa ttacccgtcg ttacccggaa ttcccctact ttacccgcct
    12901 actcccctac gcttttcact atgcttttta gcagcccaag gaacacaggc atcgtcagct
    12961 gcagtttcag ttccttggca catccggcct ggggtctgga acaaacccgt tgtgattcgg
    13021 catgcggatg ccggccagtg aatgggcatc gaggacgctg tcctctgcaa taatgccgtt
    13081 ctggtagagc aaccatgcca ccaccgagta cacctcgtcc gacgagagac tttggggagc
    13141 gggaaagggc atggcgcggt acacataatc aaagagcgtc gtggcatagg gccagtaact
    13201 accaattgtt ttcacaggct tctggctgcc taatgtgccg gcgcctccca ccaggcggtc
    13261 catcggacct tcctgacccg tcggtccatg gcaggatgca cacttggtgg caaagatcgc
    13321 agcaccttgt ttggctgtcc cgcggccgag gggtaatccc tcgcccgtcg gcgcgacatc
    13381 aatgttccac tgttgaatct ctaaatccgt ggcgggccgt cccaatccat agccatgcat
    13441 ttgatcgtgc tgatcggccg cctcactact ccagaggatg cccacgccta ggacggtgat
    13501 cagtcccatc atcatgacgt tacgcatgca cattgctcac ctctccatct gccgcaattt
    13561 tccaactctg gatcgcatta tagtggtaga cggagttcag gccacgcacg gcaaccaatg
    13621 ccgtacgact gggttgcacg tagcccgttt catcgagaca gcgactctgc agaatcacat
    13681 cctttccatc ccaccgccaa ggcaaacaga atcgtgtatg gcacttcggc agaatggggt
    13741 cttgtaagac tgcctgttgc caggaccttc cgccatccgt gctcacctcc acccgggcga
    13801 tacggcctcg accgctccac gccaaaccgc ggatgtcgat aaatcccggc tgtactcgct
    13861 gtccacccga tggagacgta atcacggatt tcgcctccat gaccatcgta aattggcgcg
    13921 ccgtgccgtc cggcattaag tccgtataat gcgacgtttc ctcgcgggtc ataaagggcg
    13981 ctgtgccgaa cttgagtcgg cgtaaccatt tgatgcatgt attcccctcc cagcccggca
    14041 caagcaggcg gagcggatac ccttgctcag gacggagggg ttcgccgttt tgcgcatagc
    14101 agaggagcgc ctcctcgatg aggggggcga gcggaaggct ccgtgccatg gccgccgcat
    14161 cgctgccttc cgccaacatc catgacgcgt ccggtttcac accgacttct tgaaggaccg
    14221 ttttcaatgc cacgccggtc cattcgcttg tgctcgtcag gccatgcgtc tgttgcacgg
    14281 tctgtcccgt cggcgccttc cattcgctcg ccgaattgcc tgaacattcg ataaatgcca
    14341 gccgcgaggt cgcgggaaac cgtttcaagt cgtccatggt gaaggcccat ggctgtgcaa
    14401 ccaagccgtg aatcatgagc cggtgacgct ccggatgaat gaccggcaca ccgttgtgat
    14461 gccgttcgaa gtgtaaggcc gacggcgtga gaattccgtg cgagtcctga agaggcgtta
    14521 aagaacccca ccatgagcgc gggaggcgga cggactgctc aaacgtcgac cgttgcccgt
    14581 acgccgttgg cgcaccaccc ggcactcgcg tcggatcatc cggcgtaacc ggcatggccg
    14641 cttcggcgaa tcttgtcacc gaatgcgtga caatcgcgct catcatgccg gccgttccgg
    14701 cgagaaggct gcgacgattc aacaaagccg ttttctcagt atccgcctcg tcgcgaggct
    14761 cgtctcgttt ttccttttgg atgctgttca tcatgggcat ctccctgatc gagcgatgag
    14821 agaaggtgct tcgtctacgc ccgatttatt catcaccttt ggagcagacc tcttgagacc
    14881 attttgggag ggctccaggc ctgccggttg ttctgcattc agcatcgact cgtgaacctt
    14941 tccaggagta cgccctttcc caatcagcaa gaaggtcact caattcgcta gaacctcgat
    15001 gccgtcagcg tcacgtccgt gctcgcccac gctcggcgga agcgcgcttc cgctgcggcc
    15061 gcttcggtgt ttttcccctg cgcccgg
//
LOCUS       DATIFR010000152         8750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1439651, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000152 DATIFR010000000
VERSION     DATIFR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439651"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VLD60_09230"
     CDS             complement(<1..443)
                     /locus_tag="VLD60_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSB45183.1"
                     /translation="MVFQNREEAGRQLAAKMQKYREIAGGLILALPRGGVAVGYQLSI
                     ALHLPLDVFITRKIGSPENPEYALGAVSETGNIYLNPDAVAEFRLSHEDVKDIVSVQR
                     REIHRRQQLYRQGRHPLVLKDRIAIVVDDGIATGATFFASVEAIR"
     gene            complement(535..690)
                     /locus_tag="VLD60_09235"
     CDS             complement(535..690)
                     /locus_tag="VLD60_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45184.1"
                     /translation="MLRHPHYLELPWDIHWREGTAAPHRHLWWLIALLVIAAMFLIGV
                     GITSGLM"
     gene            complement(790..1263)
                     /locus_tag="VLD60_09240"
     CDS             complement(790..1263)
                     /locus_tag="VLD60_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSB45185.1"
                     /translation="MSGLTRWEPFTRWSPLKELEEMEKRLSSYFGHAPATAGGEKKEA
                     ITVTEWSPLVDITEDDKEYLVKAEIPEMKKEDIKVNVHDDVLTITGERKYEKEEKGKK
                     YHRVERSYGSFMRSFTLPEDADGSKVNAEYKDGVLKVHLPKSEKAKPKAVEVKIA"
     gene            complement(1286..1519)
                     /locus_tag="VLD60_09245"
     CDS             complement(1286..1519)
                     /locus_tag="VLD60_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45186.1"
                     /translation="MASHKDIHRRVMEIIVRSRGCDLEDVVLECSDLTWNQVFLELDR
                     LSRTGRIILKQIGRGRYTVALGTGTELKARHIH"
     gene            complement(2055..2603)
                     /locus_tag="VLD60_09250"
     CDS             complement(2055..2603)
                     /locus_tag="VLD60_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB45187.1"
                     /translation="MNRRLIAIFCALAAGLSYWIAPQPYLEAKTQLSSGVIQDVDPQT
                     LKELLATFEQAEQAMQTRDLDGIMALYSDDYLYHGLKKADIRKIWSQLFDQHKELESF
                     HSFSVIRTIGGAGGKLTAEITCTGVIWGTSKVTTLRTPVDSWYEEIHYLKKENGRWRI
                     TGNVGGESQPVLQFGVAPHPLF"
     gene            2784..4127
                     /locus_tag="VLD60_09255"
     CDS             2784..4127
                     /locus_tag="VLD60_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HSB45188.1"
                     /translation="MENRASTATNVVIMGAAGRDFHNFNVVFRSDPRYRVVAFTAAQI
                     PNIAGRTYPPELAGAHYPDGIPIMPEEKLEAVIEAHAVDQVVFAYSDVSHEQVMHQAS
                     RALAAGADFRLLGPRSTMLRSGKPVISICAVRTGAGKSPLARTLAAWATEEGYRVAVV
                     RHPMPYGDLAKQAVQRFAGLGDLDEAACTIEEREEYEPHLRQGAVVFAGVDYERILRQ
                     AESEADVILWDGGNNDLPFYESDLEIVLVDPHRAGHEQRYFPGEVNLLRADVIVITKV
                     DTADPQKIEAVRRTIRSANPHAPVVESTMPLTVDDASAVRGKRVLVIEDGPTVTHGGM
                     AYGAGVLAAKQFGAALVVDPRPAAVGSLRETFRANPHLDRLLPAMGYGAEQIHDLEQT
                     VNGTDCDLVLIATPVDLRRLISIRQPTCRVGYEFRESSGELKQLVLAALKKGKST"
     gene            4194..4427
                     /locus_tag="VLD60_09260"
     CDS             4194..4427
                     /locus_tag="VLD60_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45189.1"
                     /translation="MVKVSKVSVAQQVRVDRCHRCGGLMVPERVVEMHSDDWRCVSCG
                     ERIDPVILAHRKKDVARVEAEQLFVGHSKSRLT"
     gene            4552..6174
                     /locus_tag="VLD60_09265"
     CDS             4552..6174
                     /locus_tag="VLD60_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB45190.1"
                     /translation="MRMILSHLSGSLQGKTQRYDADTVTFGTDEACGVRFDASRDTAV
                     QPVHADLAVEEGIPILHDRTGKRLIFVNGLRQAEAALRDGDLIRFGEAGPEVRFRLPP
                     DGVSSAKTLKTIMADGRDILVRTPHPRYLSPFYLVRHILIEIMMHASPLVRVVVGAVA
                     LAPLILIVWLGVVLYRQHVATITTQQQLAELVRQLEIKRLTQAELERRVDQERQLAEE
                     SRRQGEEQIAVLTAKLKAQETARESQREIAAVHQQLSAIRQSQSFAEDIIRRFGGSVG
                     LLQGGYGFKEKTTSRPLRYQGLDQLGNPYVDKDGNALVTLEGIAPPVVIYFAGTGFLV
                     DAAGTVVTNRHIVRMWELYGPAQQAIAAGFEPQMILLRLFFPGEETAYLLTEVTVPDR
                     GDIALLRTDRVPKLKTPVTLARDEPLHVGEPVVMLSYPGSVDTLLARAVSSISQDILT
                     KGGGDPVRLLDEVARKQLVRPLATQGHISDVSQDLIIYEAVSASGSSGAPLFNGAGRV
                     IGVNQAMLQRVGGVHMALPIRFANELMGSVVR"
     gene            6201..7073
                     /locus_tag="VLD60_09270"
     CDS             6201..7073
                     /locus_tag="VLD60_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB45191.1"
                     /translation="MRVVLAVDASPDSRNAAQVIRQLGEPPILDVLNVVDEDALKHAY
                     ISPTMPAGYLETYRREVTEVAEQILHEMKTELEPLCRHIRLIADSGDAAESIILTAEE
                     SHADLVIVGQRGMTATPSFLLGGVSQKVATYAPCSVLVVKEPMAKLERILVAVDGSEP
                     AHKAVEFLVRCPFKGPVQVTAVTVWPSPRSESWGIPIGTPGRSKLQQVVEEKGQELLR
                     KISKECACEAYRITTELLLGDPAFAILDAAVRCQAQVIVIGSRGMKAIKRFLLGSVSE
                     KVLVHAPCSVLIVR"
     gene            7094..8428
                     /locus_tag="VLD60_09275"
     CDS             7094..8428
                     /locus_tag="VLD60_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSB45192.1"
                     /translation="MSTKEELLAKAQKPAEESRRLHAYYRGKMQTFPKCPIRGLGDFA
                     LWYTPGVAAPCRDIQQTPGLVYDLTNKANSIAVVSDGTRVLGLGDIGPEAGLPVMEGK
                     ALLFKYLGGVDAVPICLGTKDPDEIVRTVKLLEPSFGGINLEDISMPKCFRVLEQARR
                     ACSIPVWHDDQQGTGTVLLAALINALKVVGKPIGGIRIAMIGMGAANVPTYRFLKANG
                     ADPALIVACDLGGILGLHRTDYRDTPDYAEQWKVCQETNRDGRRGGIAETLRAADVCI
                     AFAAGGIIKPEWVKTMARDAIVFACANPVPEIWPWEAKEAGAKIVATGRSDFPNQVNN
                     SLVFPAIFRGALDVRARTISDEMAVAASMELAACAEERGINEDNIICTMEEWKIVPRI
                     AAATARKAQEQGLAQLSKTTVQLMEEATARILTVREGLSHFMKEGIIPPPPI"
BASE COUNT         1816 a         2429 c         2620 g         1885 t
ORIGIN      
        1 tggcgaatgg cctccaccga ggcgaagaac gtcgctcccg ttgcgatccc atcgtccacg
       61 acgatggcaa ttcggtcctt cagcaccaat ggatgccgcc cttgccggta gagttgttgg
      121 cgacggtgta tttcacgccg ctggacactc acgatgtctt tcacgtcctc atgtgataat
      181 cggaattcgg ccaccgcatc gggattcaga taaatattcc cggtttcgct gactgcgccc
      241 agcgcatact caggattctc cggtgagccg atctttcgcg taatgaacac atccagcggc
      301 agatgcaggg cgatgctcag ttgatatccg acggcgaccc cgccacgcgg aagcgcgagg
      361 atcaggccgc cggcgatctc ccgatacttc tgcattttgg cggcaagctg acggcccgct
      421 tcttcccggt tctgaaagac cacaacactc ctcgtttgtt tggttcatct ggtttgtcta
      481 gtgtatctgg ttccataacc agacagacta aacagacgaa acatacctct caacctacat
      541 cagaccgctt gtaataccga ctccgatcag gaacatggct gctatcacca acagggcgat
      601 caaccaccag agatgacgat gtggcgccgc cgtcccctca cgccaatgaa tgtcccaggg
      661 caattccagg taatgtgggt gacgaagcat ggcaacctcc ttccctcttc caggctggcg
      721 tgaaggataa ggaagaaagc atggcactca cttatcctcc gcctgagcct tcacctggac
      781 cttcaacggt catgcgatct tgacctcgac ggccttcggt ttggcctttt ccgatttcgg
      841 caaatgcacc ttgagcacgc catctttgta ttcggcgtta actttcgagc cgtccgcatc
      901 ctccggcagc gtgaagctcc gcatgaagct gccgtacgac cgttcgacac ggtggtactt
      961 tttccctttt tcttctttct cgtacttccg ctcgccggtg atcgtgagga cgtcgtcatg
     1021 aacattgacc ttgatgtcct cctttttcat ctccgggatc tccgccttga cgagatattc
     1081 cttgtcgtct tccgtaatgt caaccagcgg cgaccactcg gtaacggtga tcgcttcttt
     1141 tttttctccg cctgccgtgg ccggagcatg gccaaaataa ctggagaggc gtttctccat
     1201 ctcttccagt tccttcaacg gactccaccg cgtaaacggt tcccatcttg tcagaccgct
     1261 catggtatct cctcctgttg ccgggttagt ggatgtgtct tgctttgagt tccgtacctg
     1321 tgccgagagc aaccgtataa cggccccgcc cgatctgttt gagaatgatc cgtcccgttc
     1381 ggctcaagcg atccagttca agaaacactt gattccatgt aaggtcgctg cactccagga
     1441 cgacgtcctc cagatcgcaa ccccgtgagc gaacaatgat ctccatcact cgacgatgaa
     1501 tgtctttatg gctcgccata atcccctctc agttttctcc tgcctcttac gcttaccctg
     1561 cattgacaat gccaggagag tgcggtgagg gagctgctgc cataccttgt acagattcaa
     1621 cgagttggac agggaagtct catgaaacaa gggcgagcga gttagtgaat tgtcgcgtta
     1681 cagggcagtg gacttctgag cgctgtggaa tctttcaaca ttgatgcggg ccgattcgtg
     1741 gttcccgaaa gccatagaaa gggaggtgag accacgataa ttcaagcttt ccgttcttgc
     1801 atttcacgca gagtgtctgg tctgtgctgg tgaggggggg gatgtccgaa ggggtgcgct
     1861 tccatctcgg tgccggttgg aggcacggct tgaagaacga gcccgatcaa tgggaagcga
     1921 gggacggtca ggcgggaggg aatcgctgga tcgttcggaa gcgtctccgg tcggtaggca
     1981 gcaggcgcgc tcaagcaaga gcggtgtttt ttgtctatgc agctacctcg gagatgtgca
     2041 cgtcatctga acggctaaaa caaaggatgg ggtgcaaccc cgaactgcag caccggctgg
     2101 gattctccac cgacatttcc ggttatccgc cagcggccgt tctctttctt cagatagtga
     2161 atctcttcat accagctatc aacgggagtc cgcagcgtcg tgaccttcga tgtgccccaa
     2221 atgacgcctg tacaggttat ttcagcagtc agcttgccgc cggctccacc aatagtccga
     2281 atgaccgaga acgagtgaaa gctttccagt tctttatgct ggtcgaaaag ttggctccag
     2341 attttccgaa tatccgcttt cttcaggcca tggtagaggt agtcgtccga ataaagtgcc
     2401 atgatcccgt caaggtcgcg cgtctgcatt gcctgctccg cctgctcgaa tgtcgccagt
     2461 aactctttca gggtttgcgg atcgacatcc tgaatcacac ctgaactgag ctgggttttt
     2521 gcttctaagt acggctgtgg agcaatccag tacgataacc cggctgccag ggcacagaag
     2581 atcgcgatga gtcgtctatt catgtgagga gcctcctgaa cgttgagcct gctcgacctg
     2641 agcataggtt ctgcaaggta gggaccatat gagatcaggc tggaacccca taattctctt
     2701 gtgaatccag gagtctcagc cctgtggagg tcacgagggg agggatatct gtagcagaaa
     2761 tcgtcagcgt tcgaggaggt ctagtggaaa atcgcgccag tacagcaacc aatgtggtga
     2821 ttatgggagc tgccgggcgc gactttcata attttaacgt cgtgtttcgc tctgaccccc
     2881 gctatcgggt cgtggcattt acggctgcac agatccccaa cattgcaggg cgtacctatc
     2941 ctcctgagct ggctggtgcg cattatccag acgggatccc gatcatgccg gaggagaagc
     3001 tggaggccgt gatcgaagcc catgcagtcg accaggttgt ctttgcctat agcgatgtct
     3061 cccacgaaca ggtcatgcat caggcctcgc gtgcgttggc cgccggagca gacttccgct
     3121 tgttggggcc ccgttcgacc atgcttcgtt ccggcaagcc ggtgatttca atttgcgccg
     3181 tcaggaccgg ggccggcaag agccctcttg cgagaacgct ggcagcatgg gcgacggagg
     3241 agggttaccg ggtcgcagtg gttcgccacc ctatgcccta tggagacttg gccaagcagg
     3301 ctgtgcagcg gttcgccggg ttgggcgatc tcgacgaggc agcctgcacc atcgaagagc
     3361 gcgaggagta tgaaccccat ctccgacaag gagccgtcgt attcgccgga gtcgactatg
     3421 aacggattct ccgtcaggcc gaatcagagg cggatgtgat tctctgggat ggaggcaata
     3481 acgacctgcc gttttacgag tccgacttgg aaattgtcct cgtggatccc catcgggccg
     3541 gtcacgaaca acgatacttt cccggagagg tcaatctgct tcgagccgat gtcatcgtca
     3601 tcaccaaggt ggatacagcc gatccacaga agatcgaagc cgttcgccgg acgatcaggt
     3661 ctgccaatcc ccacgcgccg gtcgtcgaat cgacgatgcc gcttacggtg gacgatgcct
     3721 cggccgtgcg gggcaagcga gtgctggtca tcgaggacgg tccgacagtg acgcatggcg
     3781 gaatggccta tggcgccggc gtgcttgccg cgaagcagtt cggggctgcg ttggttgtcg
     3841 atccgcgtcc ggctgcagtc ggcagtctgc gcgagacatt ccgggcgaat ccgcatctgg
     3901 accgcctcct gcccgccatg ggctacggag cagaacaaat ccacgatctg gaacagacgg
     3961 tgaatgggac ggattgcgat ctcgtcctca ttgccacgcc ggttgatttg cggcgcctga
     4021 tctcgatcag gcagcccacc tgccgagtcg gatacgaatt cagggagtcg agcggagagc
     4081 tcaagcagtt ggttctcgca gcgttaaaaa aaggcaagtc gacatgaatc cttttcatgg
     4141 aatgtggctc acacacgcaa gtcgatctgt cagcaaaaag gaggtgagga ttcatggtca
     4201 aggtctcaaa ggtctcagtc gctcaacagg ttcgtgtcga tcggtgtcat cgttgcggtg
     4261 ggctgatggt gccggagcgt gtcgtcgaaa tgcactcgga cgactggcgt tgcgtcagtt
     4321 gcggcgagcg gatcgatccg gtcattctcg cgcatcgcaa gaaggatgtc gcgcgcgtcg
     4381 aagcagaaca gctgtttgtc ggccacagca agtctcgtct cacctaagcg ccgccccctt
     4441 ccgtcctcgg gggcggtgcc agatgccgtc ccgatcattc caagctccgg tccgaacccg
     4501 tgagccgatg tccgaacgga caggctcttg tcagagaggc gcaggtctgc catgcgaatg
     4561 atcctctctc acctgagtgg cagtctccag ggcaagaccc agcgatacga tgccgacacc
     4621 gtcacgttcg gaaccgatga ggcctgtggc gttcggttcg atgcctcccg tgataccgcc
     4681 gtgcagcctg ttcacgcgga tctggccgtc gaagaaggta ttccgattct ccatgatcga
     4741 acgggcaaac gcctcatttt cgtcaacgga ctcaggcagg cggaagccgc attacgtgac
     4801 ggcgatttga tccggtttgg agaagccggc cctgaagtgc gattccggct gccgcctgac
     4861 ggagtgtcat ccgccaagac gctgaagacc attatggcgg acggtcgcga tattctcgtc
     4921 cgcaccccgc atccgcgcta cctgtcgccg ttttacctcg tccgccacat cctcatcgaa
     4981 atcatgatgc atgcatcgcc ccttgtcaga gttgtggtgg gagccgtcgc ccttgccccg
     5041 ctcatcctca tcgtctggct cggcgtcgtg ctgtaccggc agcacgtggc gacgatcacg
     5101 acgcaacaac agttggctga attggttcgt caactggaaa tcaagcggct cacgcaggcg
     5161 gaattggagc ggcgcgtgga ccaggagcgg caactcgcgg aggaatcccg gcggcagggg
     5221 gaagagcaga ttgccgtact gacggccaaa ctgaaggcac aggaaaccgc acgggagtcc
     5281 caacgagaga ttgcagcagt ccatcagcag ctcagcgcga ttcgacaatc acaaagtttt
     5341 gcggaggaca ttatccgccg gttcggtggg agcgtcggcc tcttacaggg cgggtatggt
     5401 ttcaaggaga agacgaccag tcgccccctg aggtatcagg gtcttgatca actcggcaac
     5461 ccctatgtgg acaaggatgg caatgcgctg gtgacgttgg aaggcatcgc tcctcccgtg
     5521 gtgatctact tcgccgggac agggtttttg gtggatgctg ccggaaccgt tgtcacaaac
     5581 cgccatattg tgcggatgtg ggaattgtac ggaccggctc agcaggccat cgcagcggga
     5641 tttgagccgc agatgattct gcttcggctg ttctttccgg gagaggaaac ggcctacttg
     5701 ttaacggagg tcacagttcc cgatcggggc gacattgcgc tgttacgaac ggatcgcgtg
     5761 ccaaagctga aaactcccgt cacattggca cgagatgaac cactccatgt cggcgaaccg
     5821 gtcgtgatgc tgagctatcc tggcagcgtg gataccttgc ttgcgcgggc agtctcatcg
     5881 atcagtcagg acattctcac aaagggcggt ggagatccgg ttcggcttct cgatgaggtg
     5941 gcccgcaaac agcttgtccg tcccttggcg acacaagggc acatttctga cgtgtcccag
     6001 gacctgatca tctatgaagc ggtttctgcg agcggtagca gcggggcgcc tctcttcaat
     6061 ggagccggca gggtcattgg cgtcaatcag gcgatgttgc agcgagtcgg cggggtgcac
     6121 atggcgctgc cgatccggtt tgcgaacgag ctcatgggtt cggtcgtacg gtaactccag
     6181 gaatcgagtg agggggaact atgcgcgttg tgctggccgt tgatgcttcg ccggactcca
     6241 ggaatgccgc acaagtcatc cggcagctgg gggaacctcc gatcctcgac gtcctcaacg
     6301 tggtggatga agacgctctt aaacacgcct acatttctcc gacgatgccc gccggctatc
     6361 tggaaaccta tcggcgggaa gtgacagaag tcgccgagca gatcctgcac gagatgaaaa
     6421 ccgagctgga accgctttgc cggcatatca gactgatcgc cgacagcggc gatgcggccg
     6481 aaagcattat tctgaccgcg gaggaatctc acgccgatct cgtgattgtc ggccagcgcg
     6541 gtatgacggc gacgccgtca ttcttgctcg gaggggtgtc gcagaaggtc gcgacctatg
     6601 ctccttgctc cgtcctggta gtgaaggagc cgatggccaa actggaacgg attcttgtcg
     6661 ccgtcgatgg atcggagccc gcgcacaagg ccgtggagtt tctggtgcgg tgccccttca
     6721 aaggcccggt acaggtgacg gcggtgacgg tctggccgtc tccgagatcg gagtcgtggg
     6781 ggatcccgat cggaacaccg ggccggtcaa aattgcagca ggtcgtagag gagaagggac
     6841 aagaacttct tcggaagatc agcaaagagt gcgcctgcga agcctaccgg atcacgacgg
     6901 aactgcttct tggcgatccc gcttttgcga ttctcgatgc ggcagttcga tgtcaggcgc
     6961 aggtcatcgt gattggatcg cgcgggatga aagcgatcaa acgattcttg ctcggcagcg
     7021 tatccgagaa agtattggtt catgcccctt gctcggtgct aatcgtgcga tagttggaca
     7081 agagaggttc ccgatgtcga ccaaagaaga attgctcgca aaggcgcaga aaccggcgga
     7141 agagtcgcgg cggttgcatg cctattatcg gggaaagatg cagaccttcc cgaagtgccc
     7201 gatccgcggt ctcggagact ttgccctctg gtacacgccc ggtgtagcgg ctccctgccg
     7261 ggacatccaa cagacaccgg ggttggtcta tgacctcacg aataaagcca acagtatcgc
     7321 tgtggtatcg gacggcacgc gcgttctggg gctcggcgac atcgggccgg aggccggact
     7381 gccagtcatg gagggcaagg cgctgctctt caaatatctg ggcggggtgg atgcggtgcc
     7441 gatctgtctc gggacgaaag atcccgacga gatcgtgcgg accgtgaagc ttttggagcc
     7501 gtcattcggc ggcatcaatc tcgaagacat ctccatgccg aaatgttttc gcgtgctcga
     7561 gcaggctcgg cgggcctgtt ccattcccgt ctggcacgac gaccagcagg ggaccgggac
     7621 ggtgctgctc gcggccttga tcaatgcgct caaggtggtg ggcaaaccga tcggaggaat
     7681 tcgcattgcc atgatcggaa tgggtgcggc aaacgtaccc acctatcgat tcttgaaagc
     7741 gaacggagcc gatccggcct tgatcgtcgc ctgcgacctt ggagggattc tcggtctgca
     7801 tcgcaccgac tatcgagata cgcccgacta tgccgagcaa tggaaggtct gccaggaaac
     7861 caatcgcgat ggccgccggg gcggaattgc cgaaacgctt cgagcggcgg atgtttgtat
     7921 tgcctttgca gcgggaggga tcatcaagcc cgaatgggtc aagacgatgg cgagagatgc
     7981 gatcgtcttt gcatgcgcca atccggttcc cgaaatctgg ccctgggagg caaaggaggc
     8041 cggcgcgaag attgttgcaa ccgggcggag cgatttcccc aaccaggtga acaattcgct
     8101 ggtgtttccc gccattttcc gcggtgccct ggacgtgcga gcccgcacga tttccgacga
     8161 aatggccgtt gccgcctcca tggaattggc cgcttgcgcg gaagagcgag gcatcaatga
     8221 agacaacatc atttgcacga tggaggaatg gaagatcgtt ccgcggatcg ctgctgcgac
     8281 ggcgcgcaag gctcaagagc agggattggc tcaactgagc aagaccactg tgcaactgat
     8341 ggaggaagcc acggccagaa tcctaactgt aagggaaggg ttgagtcact tcatgaaaga
     8401 agggattatc cctccaccac caatataaac tgttgcacaa agaaaacatt gattcccgga
     8461 tggcggtgct tacctaaaaa acgaaggagg tggcttgagc ctagagaggt cggcagcctc
     8521 aagatctgtg actcgccctt tcgcagccaa cgtgcaaaag gcatcgttcc tggggagttg
     8581 cggggtcata gttcagggta cgcgccatca attgtcggtg ccactaggca cggctcctgt
     8641 gggggccttt agaagatgga ggcggcgggt aatcagcctg aagcgactga cgggttagtg
     8701 gatgtgatct cggccggtgg caggtccggt gttttacgaa agggcgggcc
//
LOCUS       DATIFR010000153         8192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2591797, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000153 DATIFR010000000
VERSION     DATIFR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591797"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..588
                     /gene="nusG"
                     /locus_tag="VLD60_09280"
     CDS             52..588
                     /gene="nusG"
                     /locus_tag="VLD60_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSB45193.1"
                     /translation="MGKNWYVIHTYAGFEGRVKTSLLERANQMALTEKLGQVLVPTED
                     VIEIKDGKRRTSRRKFFPGYVLVELETPLADETLQMIKETPKVTGFVGGGAQPTPLTN
                     DEVAELLKQVDAGSAGPREQVKFIKTDNVRIIDGPFLGFNGVVDEVDHDHNRLKVMVS
                     IFGRSTPVELGFLQVERI"
     gene            686..1111
                     /gene="rplK"
                     /locus_tag="VLD60_09285"
     CDS             686..1111
                     /gene="rplK"
                     /locus_tag="VLD60_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSB45194.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKEGDSIIPVIITVYKDRTFTFVMKTPPASDLLKKAAGIIKGSGVPQKDKVGKITR
                     KQLNEIAQKKMADLNAADLEGAVKIIEGTARSMGVVVQG"
     gene            1137..1829
                     /gene="rplA"
                     /locus_tag="VLD60_09290"
     CDS             1137..1829
                     /gene="rplA"
                     /locus_tag="VLD60_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSB45195.1"
                     /translation="MGKKMKTALEKIEPRQYALREAVGLVKSAAYAKFDESVDLAIRL
                     GVDPKRSDQMVRGTTSLPHGTGKKLRVLVFAKGEKEQEARQAGADYVGSDELMEKVKG
                     GWMDFDCAIATPDLMAAVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVSEIRKGRVEYK
                     VEKAGIVQVPVGKVSFQAEQLYDNASAVLEAVVKAKPASCKGRYLKSVTISSTMGPGV
                     KLDAIALAKQWS"
     gene            1867..2373
                     /gene="rplJ"
                     /locus_tag="VLD60_09295"
                     /pseudo
     CDS             1867..2373
                     /gene="rplJ"
                     /locus_tag="VLD60_09295"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            2401..2802
                     /gene="rplL"
                     /locus_tag="VLD60_09300"
     CDS             2401..2802
                     /gene="rplL"
                     /locus_tag="VLD60_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSB45196.1"
                     /translation="MSATTTKLSQEELIKAIEGMSVLDLAELVKGLETRFGVTAAAPV
                     AVAAAPAGGGAAAAPAEEKTAFDVILASAAADKKIQVIKVVRELTNLGLKEAKDLVEG
                     APKPVKTGVAKEEADTMKKKLEESGAKVEIK"
     gene            2881..6837
                     /gene="rpoB"
                     /locus_tag="VLD60_09305"
     CDS             2881..6837
                     /gene="rpoB"
                     /locus_tag="VLD60_09305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSB45197.1"
                     /translation="MPETTLQEFVERKDYSRIRTSIDIPDLIEIQKRSYEEFLQFEVE
                     PERRKDVGLQAALASVFPIPDYNNTAILEFTSYTLGTPKYDERECLEQGMTFAVPLKL
                     RVRLVVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FAFSSDDLLKMYYPVEEIRVVKGKLMRKLDPEIHQGMRCSTEVVEKGGKEPLVREGAR
                     LTKGLIAKLKAAGVKEIPLSPSELVGRAVLSELVDAKKNKLAEKNQRLTAEMIEKIIE
                     SEIEEFKIIFFDAATTTPVILDTLEMERTASKEEAMVEIYRRLRPGETPSVETARALF
                     DNLFLNSKRYDLSPVGRLKLNKKLGLDLPLEQRTLTAQDIVEVIRYLVNLKMGKGEID
                     DIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAA
                     VKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRIC
                     PIETPEGPNIGLITSLATYARINQFGFIEAPYRKVEKGRVTEQIEFLSAIEGDKYLIA
                     QANSKVDGSGRLVSETVSARSAGDFVTVTPDKVEYMDVSPKQVVSVATALVPFLEHDD
                     ANRALMGSNMQRQAVPLLKAEAPLVGTGMEAVVARDSGYVVQAKRPGVVESVDATRIV
                     VRADSKEGRKRNDTGLDVYDLIKFQRSNQNTCITQTPVVRLGQPVKKGQVLADGPAID
                     QGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGKE
                     DITRDIPNVGEEALRNLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIF
                     GEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDTMRLQRDHHEEL
                     RIIDEEKTKKIRKLLLGKVVGRDLMDPESGDVILKKKGKLTAEILKRLSDDSVRYIIL
                     SDPDEQKELEDVERRAKEQVEILQTLYDEKVGRLKRGDELPPGVIKLVKVYISMKRKI
                     QVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHLG
                     WAARALGIKVASPVFDGAAEKEIKDLLKKAKLPTSGQTVLMDGRTGEAFGSPVTVGYM
                     YVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASTLQ
                     EFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKTQD"
     gene            6940..>8192
                     /locus_tag="VLD60_09310"
     CDS             6940..>8192
                     /locus_tag="VLD60_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSB45198.1"
                     /translation="MRIHIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVCVDPGSTDMTEKELVTEDKLRTLQSEYAPNSYKV
                     GIGADAIRELLRKIDINTKWEEIKAKAKVSTSAALKKKYAKQLKVIEAFRKSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQA"
BASE COUNT         1955 a         2037 c         2622 g         1578 t
ORIGIN      
        1 aagattctgt aaatggatga gcggacttca accaacgatc cgagggtagg catgggaaaa
       61 aactggtacg tgattcatac ctacgcgggc ttcgaagggc gagtgaagac cagtttgctt
      121 gaacgggcaa accaaatggc cctgacggag aagctcgggc aggtactggt gccgacggaa
      181 gacgtcatcg agatcaagga tggaaagcgt cgcacgtcac ggcgaaaatt tttcccgggc
      241 tatgtcttgg ttgaactgga gacgcctctg gccgacgaga cactgcaaat gatcaaggag
      301 acgccgaagg tcacgggatt tgtcggcggg ggagcgcagc cgactcctct gacgaacgat
      361 gaagtggctg aattgttgaa gcaggtggat gcgggaagtg cggggccgcg cgaacaagtc
      421 aagttcatta agaccgacaa tgtccgcatc atcgacgggc cgttcctcgg ttttaacggc
      481 gttgtcgacg aggtagacca tgatcataac cgactcaagg tgatggtcag cattttcggt
      541 cgttcgactc cggtcgagct cgggttttta caggtggagc ggatctagag ggtaggtatc
      601 acggttgaat gttgagtaga ggggacggag cttcgaggaa cgtccgcatt taacattcag
      661 cgttcaacat tcagaggagt ggcacatggc gaaagaagta tcagcgcaga ttaaattgca
      721 gattccagct ggcaaggcga acccggctcc tcccgttgga ccgtcgttgg ggcagcacgg
      781 cgtcaacatc atggaatttt gcaagcagtt caacgccaag acgcaaaaag agggcgacag
      841 catcatcccg gtgattatca cggtctacaa ggaccggacg ttcacgtttg tgatgaagac
      901 ccctccggcg tccgatctgt tgaagaaggc tgccggcatc atcaaggggt caggtgtgcc
      961 gcaaaaggac aaggtcggca agatcaccag aaagcagctc aatgaaatcg cccagaagaa
     1021 gatggccgat ctgaatgctg ctgatttgga aggggcggtc aagatcatcg aaggaaccgc
     1081 ccgaagcatg ggtgtcgtcg tccaagggta atgtcgagac agaaggagtt gcacgtatgg
     1141 gaaagaaaat gaaaaccgcg ctcgagaaga tagagccgcg ccagtacgcg ctccgcgagg
     1201 cggtgggact tgtgaagagt gcggcatacg ccaagttcga cgagtcggtc gatctggcca
     1261 ttcgcctggg ggtcgatccg aaacgatccg atcagatggt gcgaggcaca acctcgttgc
     1321 ctcacgggac gggcaagaag ctccgtgtgc tcgtcttcgc gaagggtgaa aaagagcaag
     1381 aggcacgtca ggccggagcg gattatgtcg gatccgatga actcatggaa aaggtgaagg
     1441 gcggttggat ggatttcgat tgtgcgatcg ccacgccgga tctcatggct gctgtcggca
     1501 agctcgggaa ggtgttgggg ccgcgggggc tcatgccgaa tcctaaaacc gggaccgtca
     1561 cgttcgaagt cggcaaggcc gtttcggaaa tcaggaaggg ccgggtggag tacaaggttg
     1621 agaaagccgg tatcgtacag gttcctgtcg gcaaagtgtc atttcaggcc gagcagctct
     1681 atgacaacgc ttcggcagtt ttggaggcag tggtcaaggc aaagccggct tcatgcaagg
     1741 gacgctacct caagagcgtg accatttcga gtacgatggg accaggggtg aagttggacg
     1801 ccatcgcgct tgccaaacag tggagttagt cgagttctcg ctgcggagaa ggggaagggt
     1861 tcgattatga agaaggaaga gaaagccaca gcggttgcgg agttgacgga gaagttcggt
     1921 cgtgcgcgat tggcgattgt aaccgagtgt gccgggcttt cggtgaacca ggtcaccgaa
     1981 ctgcgcaagc aactgcgggg cgccaaggcg gaatacaagg ttgtcaagaa tacgttggcg
     2041 attcgggcgg ctgaaggaac gaccctttcc ggcctgaaga gtcacttgaa ggggccgacg
     2101 ggcgtcgtga tcggctatga cgacccggtg ctgccgacaa agatccttag agatttcatc
     2161 gcggcggaaa agcgcgagga aaagatccgg attacgctcg gtgtgttgga aggcaaggtg
     2221 gtgcagcccg ctgagttggc ggccgtcgcc aagcttccga aaaaagaagt gttaatcgcg
     2281 atgctgttgt cggcgatgca agggccgatt cgcggtgtcg tttatacact gaatgcggtg
     2341 ttgtcgaagt ttgtacgagt cattgcagcc attgaggata aacggaaagg agaaggggac
     2401 atgtcagcaa caaccacaaa actctcgcag gaagaattga tcaaggccat tgagggcatg
     2461 agcgtgctcg atttggcgga attggtgaag ggtctggaga cccgcttcgg cgtgacggca
     2521 gccgcaccgg tcgccgtggc cgcagcgcca gccggcggcg gagccgcagc tgcgccggcg
     2581 gaagaaaaga ccgcattcga tgtcatcctg gcgtctgctg cggcggacaa gaagattcag
     2641 gtcatcaagg tggtccgcga gctcaccaat ctgggtctta aggaagccaa ggatctcgtg
     2701 gaaggggcac cgaagcctgt gaagacaggt gtggcgaagg aagaagccga caccatgaag
     2761 aagaagctcg aagagagcgg cgcaaaggtc gagatcaagt aagttcatcc gttcagcatc
     2821 tgcgggcatc cgtcgcggac gggtgcccga agcccaacca taggagtgga ggaccgcgga
     2881 atgcccgaaa cgactctaca agagttcgtc gaacggaaag actattctcg tattcgtacg
     2941 agcatagaca tcccggatct cattgagatc cagaagcgtt catacgagga gtttcttcaa
     3001 tttgaagtcg agccggaacg acggaaggac gtcggactgc aagcggctct ggccagtgta
     3061 tttccgatcc cggattacaa caacacggcc atcctcgagt ttaccagcta taccttgggt
     3121 acacccaagt atgacgagcg cgaatgtctg gagcagggga tgacctttgc tgtgccgctc
     3181 aagttgcggg tgcggctggt ggtattcgac aaggaagaca aggggccgaa gaaaaaagtt
     3241 ctggacgtgc gcgagcagga agtatacgtg ggcgagttgc cgctgatgac cgagcgcggc
     3301 acgtttatcg tcaacgggac ggagcgtgtc gtcgtgagcc agttgcatcg ttcgcccggt
     3361 gcgtccttca cgcatgacaa agggcgcacg catgcgagcg ggaaagtgtt gtactccgcc
     3421 cggatcattc cctaccgcgg ttcgtggctg gacttcgagt ttgacgcacg cgacatcctc
     3481 tatgtccgca tcgatcgccg ccggaagatg ccggccacga ttcttctgaa ggcgttcgca
     3541 ttttcgagcg acgacttgct gaaaatgtat tacccggttg aagaaattcg ggtggtcaag
     3601 ggcaagttga tgcgcaagct cgatcccgag attcaccagg ggatgcgttg ctcgacggag
     3661 gtcgtcgaga aaggcggaaa agaaccgctg gtgcgtgaag gggcgaggct gacaaagggg
     3721 cttatcgcga aacttaaggc ggcgggtgtg aaagagattc ctctgtcgcc gagtgaatta
     3781 gtcggacgtg ccgtgctgag cgaactcgtc gatgccaaga agaacaagct ggcggagaaa
     3841 aaccagcgcc tcacggccga aatgatcgag aaaatcatcg agagcgagat cgaagaattc
     3901 aaaatcattt tcttcgatgc cgcgacgacg accccggtca ttctggacac gttggaaatg
     3961 gagcgcacgg cttcgaagga agaggcgatg gtcgaaattt atcgccgcct gcgccccggt
     4021 gaaacaccgt cggtggaaac cgctcgagcg ctgttcgaca atctgttctt gaactccaag
     4081 cggtacgatc tctctccggt cggccgcctc aaattgaaca agaagctggg gcttgatctt
     4141 ccgctggagc aacggacgct taccgcccag gacattgtcg aagtcattcg ctatctcgtc
     4201 aacctcaaga tgggaaaagg cgaaatcgac gacatcgacc atttgggcaa tcgccgcgtc
     4261 cgctcggtag gcgaactgct ggagaatcaa ttccggctcg ggctcgtccg catggagcgc
     4321 agcataaaag agcggatgaa tctgctcgat atggagactg tgctgccgca cgacctgatc
     4381 aatgcgaagc cggtggtcgc ggcggtcaaa gagttcttca gtagcagcca gctttcccag
     4441 ttcatggatc agacgaatcc gctggcagag attacgcaca agcgacgtct ctccgccctc
     4501 ggaccgggtg gcctgacaag agagcgggcc gggttcgaag tccgcgacgt ccatccgtcg
     4561 cactacagcc gtatttgtcc gatcgaaaca ccggaaggtc cgaacatcgg gttgatcact
     4621 tcgctcgcca cctatgcgag gatcaatcaa ttcgggttca tcgaggcccc ctaccgtaag
     4681 gttgagaagg ggcgggtcac cgagcagatc gagtttctct cggcgatcga aggcgataag
     4741 tatcttatcg cgcaggcgaa ttcgaaggtg gacggatccg gaaggctggt atctgaaacg
     4801 gtttcagcac gatccgccgg cgatttcgtc acggtcacgc cggacaaggt tgaatacatg
     4861 gacgtgtcgc cgaagcaggt cgtcagcgtg gcgacagcct tggtgccgtt cttggagcac
     4921 gacgacgcaa accgcgcctt gatgggatcg aacatgcagc ggcaggccgt gccgctcctc
     4981 aaggccgagg ctccgctggt cggcaccggg atggaagcgg tcgtggcgcg ggattccggc
     5041 tacgtcgtcc aggccaaacg gcccggtgtg gtcgaaagtg tggatgccac tcggatcgtc
     5101 gttcgcgccg attcgaagga aggtcgcaag cggaacgaca ccgggttgga cgtgtatgac
     5161 ctgattaagt tccaacgttc caatcagaat acctgtatca cccagacacc ggtcgtccgt
     5221 ctcgggcagc cggtgaaaaa ggggcaagtg ctcgccgacg gccctgcgat cgatcagggg
     5281 gagctcgcgc tcggcaagaa cgtgctcgtg gccttcatgc cctggggtgg atacaacttc
     5341 gaagacgcca ttctcttgag cgagaagctg gtgcgcgagg acgcctttac ctcgattcac
     5401 attgaggagt ttgaggtgga agcgcgcgat accaagctcg gcaaggaaga tattacgcgc
     5461 gacattccca atgtcggtga ggaagcgctg cgcaatctcg atgagagcgg catcattcgc
     5521 atcggcgccg aggtgaagcc gggcgacatc ctggtcggga aggttacgcc aaaaggcgaa
     5581 acccagctca cgccggaaga gaagctgctc cgcgcgattt tcggtgaaaa ggccggtgat
     5641 gtgaaggata cgtccctcac ggtcccgccg ggcgtcgaag gtattgtggt cgatgtgaag
     5701 attttctccc gaaagggact cgacaaggac gagcgttcca agagcatcga gagcgaagac
     5761 accatgcggc tccagcgtga ccaccacgag gagttgcgga tcatcgacga agaaaagacg
     5821 aagaaaattc ggaagctgct gctcggcaaa gttgtcgggc gggatttgat ggatcccgag
     5881 agcggcgatg tcattctcaa gaagaagggc aagctgacgg ccgaaatcct caagcggtta
     5941 tccgatgact ccgttcgcta catcatcctc agcgatccgg acgagcaaaa ggaattggaa
     6001 gacgtcgagc ggagggccaa ggagcaggtc gaaattctcc agacgctcta tgacgagaag
     6061 gttgggcggt tgaaacgggg cgacgaattg ccgcctggcg tcatcaagct cgtcaaagtc
     6121 tatatttcga tgaagcgcaa gattcaggtg ggcgacaaga tggccggccg gcacgggaac
     6181 aaaggcgtgg tctcacgggt cctgcccgag gaggatatgc cctatttgcc ggatggaacg
     6241 ccggtcgaga tcgtgctgaa tcctctcggc gtgccgtccc gtatgaacgt cgggcagatt
     6301 ctggaaaccc acctgggttg ggccgcgagg gcgctcggta tcaaggtagc aagcccggtg
     6361 ttcgatggtg cagcagaaaa ggaaatcaag gatctgctca agaaggcgaa attaccgacg
     6421 agcggacaga ccgtccttat ggatggccgc accggagagg cctttggcag tcctgtgacg
     6481 gtcggctata tgtatgtcct gaagctccac cacctggtgg acgacaagat tcacgcacgg
     6541 tcgattggtc cgtactctct cgtgacgcag cagccgttgg gtggtaaggc ccagttcggc
     6601 ggccagcggt tgggagaaat ggaagtctgg gcgttgcagg cctacggagc ggcctcgacg
     6661 ctgcaagagt tccttaccgt gaaatccgac gacgtgccgg gccggtcacg catgtatgaa
     6721 gcgattgtga aaggcgaacc cttcctggaa ccggggttgc ccgagtcatt taacgtcttg
     6781 gtcaaagaac tgcagagttt ggggctggac gtggagttgg tcaagacgca agactaacca
     6841 cttgtgtgcc ggctgcggcc ggtatcagag gaggtcacta ccttggaagg cgtatataca
     6901 ttgttcgaaa agccacggga ttcggtgtcg ttcgattcga tgcggattca catcgcgtcg
     6961 cccgagaaga tccggtcctg gtcatacggc gaggtcaaga agccggagac gatcaactac
     7021 cgctcgttca agcctgaaaa ggacgggctg ttctgcgcga agattttcgg tccgatcaag
     7081 gactgggagt gcaattgcgg caagtacaag cgcatgaagc accgcggtat cgtttgcgat
     7141 aagtgcggtg ttgaggtgat tcaatcgaaa gtacgtcgcg agcgcatggg ccacatcgag
     7201 ctggcggcgc cggtcgcaca tatctggttt ctgaagggcg taccgagccg tattggaacc
     7261 ttgctcgata tgagcctcaa acagctggag aagattctct acttcgaaag ctatgtctgc
     7321 gtcgatccag gctccacgga tatgacggaa aaggagttgg tcacggaaga caagcttcga
     7381 actctccagt cggaatacgc gccgaattcc tacaaagtgg ggatcggagc cgacgccatt
     7441 cgtgaattgc tgcgcaagat cgacatcaat acgaagtggg aggagatcaa ggcgaaggca
     7501 aaggtctcca cctctgctgc attgaagaag aagtacgcca aacagttgaa agtcatcgag
     7561 gcgttccgga agtccggcaa taagccggag tggatgatca tggacgtgat tccggtgctc
     7621 ccacccgaat tgcgtccgct tgtcccgctg gacggcggtc gattcgccac gtccgatcta
     7681 aatgacctct accgccgcgt catcaatcgg aacaaccggt tgaagcgttt gatggaattg
     7741 aaggctcccg gtgtgatcat ccgcaacgaa atgcggatgt tgcaggaggc ggtggacgca
     7801 ttattcgata atggtcgccg tggtcgagcg attcgtgggc ccaacaagcg gccgttgaaa
     7861 tcgctcagcg acatgctcaa aggcaagcag gggcgattcc gtcagaatct tctgggtaaa
     7921 cgcgtggatt attccggccg tacggtcatc gtggtcggtc cggagctccg actccatcaa
     7981 tgcggtttgc cgaagaagat ggcgctggaa ctgttcaagc cgttcatttt ccataagctg
     8041 gaagagcgag gcgcggcgac gaccatcaag agcgccaagc gtctggtcga aaaggagcgt
     8101 cctgaagtct gggacgtatt ggacgaagtc attcgtgaac atccggtgct gttgaatcgt
     8161 gccccgacct tgcaccgatt gggcattcaa gc
//
LOCUS       DATIFR010000154         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1824719, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000154 DATIFR010000000
VERSION     DATIFR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824719"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VLD60_09315"
     CDS             <1..376
                     /locus_tag="VLD60_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45199.1"
                     /translation="GTAATAHGALLTGNAAASLMSGSDDAPGSSSSGEPLVGNNPREA
                     KGRTNTDLPGGHKAATDKFGELIKGQKVTVDPKTGHQVAADGTRLRLNPDGTARVDIP
                     KSNNRPKNETVHFNDPDKLRQP"
     gene            388..948
                     /locus_tag="VLD60_09320"
     CDS             388..948
                     /locus_tag="VLD60_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45200.1"
                     /translation="MEGESLADFLSRAPMQSVVGMPLGDVQGFYLDIVLASGDASVRL
                     TGTAFDLPDRTEAFLLTASDFNPEEWWTGPGGQPGWKAEDLLGTWTKFGKGRIWQGPR
                     LVRYFVDPDDRQRGLPMQGPLDSAAAIEFGGEQGRRTDRLVLYATPEYPCAIELATTI
                     ERCDAILASLQEFAPQTARTTRVAGI"
     gene            complement(1120..2592)
                     /locus_tag="VLD60_09325"
     CDS             complement(1120..2592)
                     /locus_tag="VLD60_09325"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HSB45201.1"
                     /translation="METNDNPRFEPINRQQVVLEPLDIEQLIPQDHPARSVWEILGRL
                     DLSRFVGDVKSVEGHAGRNVWEPRLLIAIWIYAYSRGVSSAREIERQCAYEPALRWLT
                     GLKVVNHHTLSDFRVQHGDALQNLFVQVLGILAMEKLVTLERVTVDGTKVRACVNKKT
                     FSRAHKIREHLKLAREHIEELQREEVEQEKRGRQAAARKRAARERVHRLEEALAEVER
                     LQAEKKGEREKPCQASVSDADAQFMWTSDHGLAPSYNVQLVTDAAQKLIVDVEVSKQP
                     SDSYHLLPALDRVERQFGAYPQQAIADGDYTKREAVMGAAERGIDYYGSWGAVPEDRL
                     PYGIDAAYHPARFPYDERHDVLICPEGKHLLRKSSREKAGVEVHVFVAPRRDCQACAK
                     RNLCTPQNRMVKHGRAVTIRVEHESVECYHAKMRTAKAKQIYKQRAPIAEFPHAWLKD
                     KLKWVRLRCRGLANARAEALWTCLTYNLQRYFKLRVLQTA"
BASE COUNT          557 a          861 c          793 g          583 t
ORIGIN      
        1 tggaaccgcg gcgacagctc acggtgcatt gctaacgggt aacgcagccg caagcctaat
       61 gagcggctca gatgatgctc caggaagcag tagttctggc gaacctctcg ttggcaacaa
      121 tccgagagaa gccaagggca gaaccaacac tgatttgccc ggaggccaca aggcagcaac
      181 ggacaagttt ggagagctga tcaagggcca gaaggtaaca gttgatccga aaacgggcca
      241 tcaagtggca gccgatggaa cgcgtctccg ccttaacccc gacggcacgg cgcgtgtcga
      301 cattccgaag tctaacaatc ggccgaaaaa cgaaacagtc catttcaacg atccagacaa
      361 gctgaggcaa ccgtgaggga gcgacgtatg gaaggcgaga gtcttgccga tttcttgagt
      421 cgcgctccaa tgcagtctgt ggtcggcatg ccgctaggag atgttcaagg cttctacttg
      481 gacatcgtgc tcgcgtccgg cgacgcatcc gtacgtctca ctggaactgc tttcgatctc
      541 ccagatcgca ccgaggcctt tctgttgact gcgagtgatt tcaatccaga ggaatggtgg
      601 acagggcctg gcggccagcc aggttggaag gccgaggacc tgctgggtac ctggacgaag
      661 tttggaaagg gaagaatctg gcaggggcca aggttggtcc gctactttgt tgatccggat
      721 gacaggcaac ggggattacc gatgcagggt cccctcgatt cagcggctgc cattgagttt
      781 ggaggggagc agggccgccg aaccgaccgg ttagtgctct acgcaacccc cgagtaccct
      841 tgcgctattg agttagctac cacgatagag cgatgtgacg cgattctcgc aagtcttcag
      901 gagtttgctc cgcaaacggc taggacgact cgcgttgcag gcatatgatc gcccggaaac
      961 ctggggtgac cttcggacgg gaagctgtga aacaatcggc tccgcgggcg tcatttcggc
     1021 gcagaacctc cttcgctcgg ctgaaccgag cgcggtggcc cgcgctggcc tctgcggtgg
     1081 caacccgcgt ggttgccgct ccggtcttcg aatccgcact cacgccgttt gaaggaccct
     1141 tagtttgaag taccgttgca ggttgtacgt gaggcaggtc cagagcgctt cagctcgggc
     1201 attggctaac ccccgacagc gtaggcgcac ccacttcagt ttgtctttga gccacgcatg
     1261 cggaaactcg gctatcggcg cacgctgctt atagatctgt ttggctttgg ccgtccgcat
     1321 tttggcgtgg tagcactcca cggattcgtg ctccacgcgg atcgtcacgg cgcggccgtg
     1381 cttcaccatg cggttttgcg gcgtgcacag attgcgcttc gcacaggcct gacaatctct
     1441 tcgcggggcc acgaacacat gcacctcaac tcccgctttt tcccgactgc tcttgcgtaa
     1501 cagatgcttt ccttccggac agatcaagac atcgtggcgc tcgtcatacg ggaacctggc
     1561 gggatgatac gcggcatcga ttccgtaagg cagacggtcc tccggcaccg ctccccagct
     1621 tccgtagtaa tctattccgc gctcggctgc gcccatcacc gcttctcgct tggtgtaatc
     1681 cccatcggcg atggcctgct gcgggtaggc cccgaactga cgctcgacac gatccagcgc
     1741 cggcaacagg tgatacgaat ccgagggttg cttgctgacc tccacatcca cgatgagctt
     1801 ttgcgcggca tcggtaacca attgcacgtt gtaggaagga gctaaaccgt gatcggaggt
     1861 ccacatgaac tgagcgtccg catcactgac cgatgcctgg caaggcttct cgcgctcccc
     1921 cttcttctcc gcttgcagcc gctccacctc cgccagcgct tcttccaagc ggtgcacccg
     1981 ctcgcgcgcc gctcgcttcc gggcggcagc ctgccgtccc cgcttctcct gctcgacttc
     2041 ctctcgctgc aactcctcga tgtgttcccg cgccagtttg agatgctccc ggatcttgtg
     2101 ggcgcgggag aacgtcttct tgttgacgca cgcccgcact ttggtcccgt ccaccgtcac
     2161 ccgttccagt gtgaccagtt tctccatggc gagaatcccc agaacctgca caaaaaggtt
     2221 ctgcagagca tcgccgtgtt gcacgcgaaa atcggaaagc gtgtgatggt tgaccacctt
     2281 cagcccggtc agccaacgca gggccggttc ataggcgcac tgccgctcga tctcccgagc
     2341 cgaggagaca ccccgcgaat aggcatagat ccaaatcgcg atgagcagtc gcggctccca
     2401 gacgttgcgc ccggcatggc cctcgaccga cttcacgtcg cccacaaacc ggctcaggtc
     2461 caggcgaccg agaatctccc aaacgcttcg tgcggggtga tcttgcggga tgagctgttc
     2521 aatgtcgaga ggttcgagca cgacctgttg gcgattgatc ggttcgaaac gcggattgtc
     2581 gttcgtttcc acgcttccag tctacggatg ccaaaacaga aaacaagtga aatgttcgat
     2641 tatttcacag cttccagacg gaacgttttc tggcacggtt tccatgagac cctttctgcc
     2701 cttcagacac cacgtccata tggctcgctt gacgcgcctt gttgcgatcg acgccccaca
     2761 tcaccaatct tcccctgcaa cgctggccaa agct
//
LOCUS       DATIFR010000155         1629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1633151, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000155 DATIFR010000000
VERSION     DATIFR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633151"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VLD60_09330"
     CDS             <1..457
                     /locus_tag="VLD60_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45202.1"
                     /translation="VIGYAVADACAIATIDGDGKINIGPLCKIDTSPGLPSELCWLAI
                     SPDDRLVYAANFGYSYVSALRINGSSLEIAKDPACPKVPGDGMVRGLNTTITSGPSDN
                     WITPDGAYLYQIYGNASKLVGYGARPDGSLEEITRVTIPYNSPQGLVGF"
     gene            472..663
                     /locus_tag="VLD60_09335"
     CDS             472..663
                     /locus_tag="VLD60_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSB45203.1"
                     /translation="MSKETKEMHPEGREVVDRRAFLKMVAGAAAGAGVVAGMPEIAAA
                     QTSKSPVDWMGSPADDPEC"
     gene            694..>1629
                     /locus_tag="VLD60_09340"
     CDS             694..>1629
                     /locus_tag="VLD60_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45204.1"
                     /translation="MKSSKLLGRSICGPMMVLFTSFFAMDVSSAADTSTEKRITKQFL
                     TSGQVDLAREVVKLPLHRGRLSSGETVWFVLTDVSDFATSKKMGLTWAPSLSKAKDLA
                     STRVAEMDESNNFTFKSGRVDFSPTQMLAAGKAPNFFPPRMARAGSIGDAHYSPLVRV
                     ANRNDIVFNAPLIAFNVGPEKISFCGGNPDYSVVTDSVANICPENGTVDLKLRFGFAD
                     GKHLRYLSFDANSEESAVLEASTFAPAESDILKSGATEIIYTIVNGKTGQNDPERQGL
                     NSALSGEGPSLDILAQFKEISAGYSPMWDVQLAEWT"
BASE COUNT          383 a          446 c          480 g          320 t
ORIGIN      
        1 cgtcatcgga tacgccgtcg ccgacgcatg cgccatcgca actatcgatg gagatggcaa
       61 gatcaacatc ggcccgctct gcaagatcga cacgagcccg gggctgccct ctgagctgtg
      121 ctggctggcg atttccccgg atgaccggct cgtgtatgcc gcgaacttcg gctacagcta
      181 cgtcagcgcg cttcgcatca acggcagcag cctggaaatc gcgaaggatc cagcatgccc
      241 gaaggttccg ggggacggca tggtcagggg actcaacacg accattacca gcggaccgag
      301 cgataactgg atcactccgg atggagcata tctctaccag atctacggga atgcctcgaa
      361 actggtgggc tacggggcac ggccggacgg ttctcttgag gaaatcacca gagtgacaat
      421 tccctacaac agccctcagg gcttggtggg attctgaaag gagaaataag tgtgtcgaag
      481 gagacaaagg agatgcatcc ggaaggccgg gaggtcgtag atcgtcgagc gtttctcaag
      541 atggtagcgg gcgcggcagc tggggctggc gtcgtcgctg ggatgccgga aattgctgcg
      601 gcacagacga gcaaatcgcc ggtagattgg atggggtctc cggcagatga tccagagtgc
      661 taaatccgag taacgccgac gagaggagcg atcatgaaat ccagcaagct tttaggcaga
      721 tccatttgcg gtcccatgat ggtcctgttt acgtcttttt tcgcaatgga tgtaagttcg
      781 gcggcggaca catcgaccga aaagagaatc acgaagcagt ttctcacgtc aggccaagtc
      841 gaccttgcaa gagaagtggt gaaactgccg cttcatcgcg gtcgcctttc ctcgggagag
      901 actgtttggt tcgtcctcac cgacgtaagc gatttcgcca cttccaagaa aatgggtctc
      961 acatgggcgc cgagtctcag taaagcaaag gatctcgcaa gcacgcgggt tgcggaaatg
     1021 gatgagtcga ataacttcac cttcaaatca ggtcgggttg atttctcacc aactcaaatg
     1081 ctagcggcag gtaaggcgcc aaacttcttc ccacctagga tggcccgagc cgggtccatc
     1141 ggggatgcgc actacagccc gttggttcgg gtggcaaacc gaaatgacat tgttttcaac
     1201 gctcccctga tcgcgttcaa cgtcggacca gagaaaatct ccttctgcgg cggcaatccc
     1261 gattactcgg ttgtgaccga ctcggtggca aacatctgcc ctgagaatgg caccgtcgac
     1321 ttgaagctgc gcttcggatt tgcggacggc aagcacttgc ggtacctcag cttcgacgcc
     1381 aactccgagg agtcagcagt gctcgaggca tctacctttg cgcctgccga aagcgacatc
     1441 ctgaaaagcg gcgcgacgga gatcatctac acaatcgtaa atggcaaaac aggccagaac
     1501 gaccctgagc gccaagggct caacagcgcg ctcagtggcg aaggcccttc actcgacatc
     1561 cttgcgcagt tcaaggagat cagtgccgga tattcaccga tgtgggatgt tcagctggcc
     1621 gagtggacc
//
LOCUS       DATIFR010000156        19581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1252004, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000156 DATIFR010000000
VERSION     DATIFR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19581
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252004"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1932)
                     /gene="dnaK"
                     /locus_tag="VLD60_09345"
     CDS             complement(7..1932)
                     /gene="dnaK"
                     /locus_tag="VLD60_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSB45205.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVGITD
                     KNERLVGQIAKRQAITNPENTIFSVKRLMGRKFRSKEVQEALKRLPYKVVEADNGDAH
                     VELRGKRYSPSEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDISILEIGEGVFEVKSTNG
                     DTFLGGDDFDQRVMDWLVDEFKKDQGIDLRKDRMALQRLKEAAERAKIELSSSQESEI
                     NLPFITADASGPKHMVIKLTRAKLEQLVDDLIQRTIDPCKKALADAGVSGRDIQEVVL
                     VGGMTRMPKVIQVVKEFFGKEPHRGVNPDEVVAIGAGIQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQIFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGLPPAPRGMPQIDVVFDIDANGIVHVSAKDLATQKEQSIKITASSGLSKEEVE
                     KLVRDAQSHTEDDKKRRKAAEAKNQADNLIYQTEKNLKEHGDKIAADDKTKIEEAVTA
                     LKKAMEGNDPEAIESATQTLTTASHKLAEEMYKKASASAAAGAGADSAGGESPNGAKT
                     DEKVVDAEFEEVDKEKK"
     gene            complement(1985..2566)
                     /gene="grpE"
                     /locus_tag="VLD60_09350"
     CDS             complement(1985..2566)
                     /gene="grpE"
                     /locus_tag="VLD60_09350"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSB45206.1"
                     /translation="MAEEEKITNTIDNLEAGSTGEISSDVLAIQQALTSKTEELKALN
                     DKYLRLAAEFDNYKRLAQRDQRDQIRFGNEQLLKELLPVVDNLERAIKAAKNGGNGEA
                     LVQGVDLTLKQLVGSLTKFGVRPMQTVGQPFDPSGHQAVASVPSDEVPKQHVVEEFQR
                     GYRLHDRILRAAMVAVSTGAASANGTNDTPNES"
     gene            complement(2661..3452)
                     /locus_tag="VLD60_09355"
     CDS             complement(2661..3452)
                     /locus_tag="VLD60_09355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSB45207.1"
                     /translation="MLLVIDIGNTNVVLGVYDGAVLKAHWRLATDAKTTSDEYGILLI
                     NLLASARLLPVHISAAIMSSVVPALTSTFETLIQQYFHQRPMVVTTETDTGLRLRYAN
                     PKEIGSDRLVNAAAAYHKYRRDLIVVDFGTATTFCTITKEGEYLGGVIAPGLGISAEA
                     LFARAAKLSKVELTKPKTVIGVDTASSIQSGLLFGYAGLVDALVHRIEQETGRSAYVI
                     ATGGLSTVIVPETTTIHKIEPMLTLEGLALLYRRATGNLSSADWV"
     gene            complement(3486..4325)
                     /locus_tag="VLD60_09360"
     CDS             complement(3486..4325)
                     /locus_tag="VLD60_09360"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSB45208.1"
                     /translation="MQPSPPLDIQAIQSTLATKSLGRRIELHAQLGSTNREAVALAQG
                     GVEHGTIVLAEAQTKGRGRLSRSWFSPPGVNIYCSIILRKTIGRERLAEWLSWLPLVT
                     SLAVAEAIEAVTFANVAVKWPNDLVIAERKVGGILCETGTSRQSEPFQVVGIGLNVNG
                     ALADFPPDMRDMATTLAHETGRSVDRNRLLAQLLGELETCVEELATHGSERIALAYQR
                     RCRTVGKVVKAMLTEGKEFVGLAESIGRDGSLTVVQRPIPEEGQSPETVHLRVADIIH
                     LRT"
     gene            complement(4329..5201)
                     /gene="nadC"
                     /locus_tag="VLD60_09365"
     CDS             complement(4329..5201)
                     /gene="nadC"
                     /locus_tag="VLD60_09365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSB45209.1"
                     /translation="MVRLPAADIRRAVRQGLEEDLGQGDLTTSALFKIRIPARARIIA
                     QQPLVIAGMAAALQTFVTVDPSLTFPVVQKDGAHAKNGDTLLEIHGDGRSILMAERVA
                     LNFLQHLSGIATMTQRFCQAVRGYPVAVLDTRKTLPGWRSLQKWAVALGGGTNHRRSL
                     GDGILIKDNHLALMKKKPAVLTACRLAKATAGHSTIVEVESLAEVKQALNGHADIILL
                     DNMTPAMVKKAVRLIKGRALVEVSGGITLKNVRSMAAAGPDRISIGALTHSAPAATMS
                     LELTPFTQSRTRHP"
     gene            complement(5203..7974)
                     /locus_tag="VLD60_09370"
     CDS             complement(5203..7974)
                     /locus_tag="VLD60_09370"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSB45210.1"
                     /translation="MSTPQLDKTYDPKAVEARWYATWIERGYFHASTTHPGQPYCIVI
                     PPPNVTGSLHVGHALNHSLQDILIRWRRMQGRNTLWLPGTDHAGIATQNVVEKQLMAE
                     GTSRESLGRERFIERVWQWKATSGNTIVTQQKQLGESCDWERLRFTMDEGLSKAVLEV
                     FVRLYEDGLIYRGERLINWCPRCLTALSDIEVEHEDIKGKLYSIEYPLEEDPAIKLTV
                     ATTRPETMLGDTAVAVHPEDDRFKHLIGKRVRLPLTNRAIPIVGDAILVDREFGTGAV
                     KITPAHDFNDFEAGERHRLPRLAIFDHQALLDADKLRDASVDHAVIDALRGLPVTKAR
                     PKVEGLLRNLGALVKIEDHKMALGKCYRCKTVVEPYLSPQWFVKIQPLADPAIKAVED
                     GRIRIIPEGWTNNYLGWMRDIKDWCISRQIWWGHQIPAWYCETCNSTLIHKQVTTSND
                     GLPVVRLTLLQGAKPIVARSAPTRCSTCGGQNFLRDPDVLDTWFSSGLWPFSTLGWPE
                     QTPELKRYYPTSVLVTGLDILFFWVARMIMLGLKFMEDVPFRDVYIHALVRDAEGQKM
                     SKSKGNVIDPLHVMEQFGTDALRFTLASMASPGRDIKLAEERIEGYRNFANKIWNAAR
                     FSLMYLDGPKAAHPSTARSFPDRWILSRLNHTIRTITSELEAYRFDRAASVLYHFIWH
                     EYCDWYLELIKPALQDAAHPDGPGARQTLVETLETTMRLLHPFMPFLTEEIWQTIPHQ
                     GDSIVIQSYPAPDETWRAPEAEELFTLLEKTVGLVRTGRVLLNYPPGQPISFYMSHDD
                     PTRNSRLHQLQRNLAHLGRGTAELAPRTDWPTVKLLRLVTEGLSVGLVVSGDVDLKKA
                     LDRVVKQQDEQEKEMGRLKGKLGNQEFVAKAPPDVILDHQTRLHSLQHDQTMLASSEQ
                     QLRAMLGTS"
     gene            complement(8278..9435)
                     /locus_tag="VLD60_09375"
     CDS             complement(8278..9435)
                     /locus_tag="VLD60_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45211.1"
                     /translation="MASTIFVIDSSPAVRRMVEQISTPAGYDVVGFQDGPAALEAARK
                     LSPNLIITDYHLDNMTFSGFCKEVHKLDNLTDTYIVALTGATDKFDESHLKSLGVKAF
                     LKKPFQSENLLDIIKDLDRAKASTNGGKKKRSWPPTSSATDYDDDESPDGLADEEQVG
                     DEDINLTIPFPVQTKVTDKKETPMPSTPTAKQATAEPEEAMKGLFGQLLQSMSERTEK
                     KLTDLLPQMVTKELAGLVAKAVETEVNQQLGAGLSQERLTQVIEPLLAKELPKVLISE
                     MATLEPIIRHSIFEIASPLIKDNVDQMVREQADAVKASLPEAVREHLKSIEGQVQEEI
                     KQAATAQAEKLAGDIVRAAADEKIQQAVQKLVPAIIEEQIKAEITRLTESS"
     gene            9576..10295
                     /locus_tag="VLD60_09380"
     CDS             9576..10295
                     /locus_tag="VLD60_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSB45212.1"
                     /translation="MSQPIQVVFFDAANTLFHVNGSVADIYLQHAVQFGFQQTPDSRA
                     AITQAFSRAFNEAPPPIFAATEPAHIKQSERLWWFDVVHNVFYRVGMFERFDDFFERV
                     FEVFEDPGSWKLFPETEQTLAGLRKQGMELGVISNFDSRLFSVMRGLGIHELFDTVTI
                     SSMAHAAKPAPKIFQLALDKHAVDPDEALHIGDSVRDDVEGAVNAGLHALLLDRQGRH
                     QGSGVRVIRTLDELLPVIERL"
     gene            complement(10290..11072)
                     /locus_tag="VLD60_09385"
     CDS             complement(10290..11072)
                     /locus_tag="VLD60_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HSB45213.1"
                     /translation="MAEYPEAALLHPLYRILTAMAPGSDLAILREHIAKARSLTILTG
                     AGISADSGVPTFRGTEGLWRQYRAEDLATPEAFERDPRLVWEWYNWRRERIAGTQPNP
                     AHHAVVELERRIRSFWLITQNVDGLHRAAGSQKLSEIHGNIWMVRCTACGSISENRDM
                     PISLLPTCPHCRGLLRPHIVWFGESLFPDDLARCAAVLQTCEILLVIGTSGVVYPAAG
                     FASVAKEAGAFVAEINLEPTPHSELVDFSLQGRAKDVVPLLL"
     gene            11115..12485
                     /locus_tag="VLD60_09390"
     CDS             11115..12485
                     /locus_tag="VLD60_09390"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HSB45214.1"
                     /translation="MKQILMVGAGSVGGFFGAHLAKSNSNVSFLLRPKTLAAVQQHGL
                     TIRSAAGTFTVRPRAASDARQLPRPDLIILGVKAYDLDEVMAQVEPVLSDKTVILTLQ
                     NGIDTEDRIIARLKRDCVVGGVAFIYSKIAAPGVIDHYKKGAVAIGELMGHESERLLQ
                     IRDIFAAAGIPCQISKDIRRSKWEKMCWNCVFNPITVLIDDKVSKALDHPEMMGVIRQ
                     IVGEITAVSAALKVPLPADMPERVVKWTQEIRDIHTSMYDDWKAGRQTEIRNLNGYIV
                     EQGRALGIPTPVNEALTAMIKTMTEMEKTGSGVVRIDGAVVQPVSLDRTAMRQLPGEH
                     QVADVSTVMPSMRGRAITLKGLLDVPALGIQADHVTFHSSDGKYAATLTLQQAKDFGM
                     LLYELDGEPLPAGKGGPFRLITPGLGDLCANVKGVGRIEVRVGSGKDTRPSERPPECT
                     ADAQSS"
     gene            complement(12488..13813)
                     /locus_tag="VLD60_09395"
     CDS             complement(12488..13813)
                     /locus_tag="VLD60_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HSB45215.1"
                     /translation="MANGVTQIRRSVLTLALPVTVSSLLQRTEGIVAVFLVGGLGAIP
                     IAAVGLGQLLAFIATTLVSGLAVGSNVIVAQLWGARRHREAGQAARHFLGLSIAVSLI
                     LMLSGMLLNRFAMQQLGAESAVMALAHPYSTLIFLVIPFTVLIQVLSSILQGTGDTKT
                     PMYAMIVVNILHIAIAYPLIYGRWGLPAVGVQGAAIAVGVAEATGALYLFQRCRPLLH
                     RSTHVRFDLFRTMWQVGAPVSGERIVQQAGILLYTKIVLLYGTVSYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGAGKYTRAKLENWEANRMATIVMSAMGVVFFFFPYALL
                     RAFTSDEAVIDLGTVFLKIVALLQIPLALTMVLAGSLRGAGDTHFIMVATTIGMWAMR
                     VPIAFITGVWFELGVFYVWLAMIADWSLRMALMLWRYRSERWKAIQVIR"
     gene            13888..15423
                     /locus_tag="VLD60_09400"
     CDS             13888..15423
                     /locus_tag="VLD60_09400"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HSB45216.1"
                     /translation="MTELHDLSPAPASTPPRLSKSKFLSGLQCHKRLHLEIHQPQLAT
                     PPDPGTQAILDMGTEIGELARNRFPGGVLVTAGYRQRDEALARTAALLQDPKVPAIFE
                     AALQHDGVLVRVDILERVPAGAGEPPAWRLIEVKSSTRVKDVHVDDLAIQSQVLSGAG
                     LALAGTYLMHIDTSYLYEGGEVDLETLFAIEDLSEVVAGRRTHVTEQLAAMKAMVLLP
                     DPPPIEPDHHCHSPYVCPFWAHCTKEKPERWIYHLPGSKQTVSRLVQQGITTIDEIPD
                     TANLSLAQRRVKDNVEWVSPKLGSLLRSVQYPVHHVDFETVMLAVPRFATTRPYQSIP
                     VQWSNHIELESGELLHQEFLHGDPTEPRRRWAEALIESLGEKGSIVVYSAYEEAMIKQ
                     LVEAFPEFRTAFNPIMKRLWDLHPIIKDHYYHPSFQGSYSIKSVLPAVVPSMGYEDLA
                     IKEGGQAASEYYRMVFVETDWIEQSTIKEALLRYCARDTLAMVELRRVLGEKANGGGR
                     ESS"
     gene            complement(15806..15895)
                     /locus_tag="VLD60_09405"
     tRNA            complement(15806..15895)
                     /locus_tag="VLD60_09405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15859..15861),aa:Ser,seq:cga)
     gene            complement(16257..17021)
                     /locus_tag="VLD60_09410"
     CDS             complement(16257..17021)
                     /locus_tag="VLD60_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45217.1"
                     /translation="MARRGDGLYLRGIMWYLDCRINGARHVVRLGKNIQRTTAGELAN
                     VKRAAILRGEAGIGKKRKDISIEKAKEAFLDWTVTNKRPRTVRVYRQQLERLAISFSG
                     KTLGQISSFDVERHKRDWAEQGARIQANREIAVLKNLVNKAKTWGHYEGENPALGVKM
                     QEEPKRRLRYFDANGEPKLLEVSPEPLRSLIIIGTNTGIRIAARGAHPKMIGRGSRPR
                     PPHGASRLCQERTDEKHTVEQPSQSGVSPAQENQSE"
     gene            complement(17052..17483)
                     /locus_tag="VLD60_09415"
     CDS             complement(17052..17483)
                     /locus_tag="VLD60_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45218.1"
                     /translation="MKEKTEKTKVLAVCTGNRCRSKTLAGMLNATPGCEARSAGTNPI
                     LAGRSITEEDLRWAGHVIVFEDYHVQCIRSRFSKLAKKLDIINLDIEDRYAPFDPVLI
                     ELLKDNVARRLGLALSVPDNASALYQEDLKENGPFYPSSRW"
     gene            complement(17511..17633)
                     /locus_tag="VLD60_09420"
     CDS             complement(17511..17633)
                     /locus_tag="VLD60_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45219.1"
                     /translation="MKKKMRGSIAKVEPVGTGVTQHKLKLTFADGTQAIFKPAS"
     gene            complement(17859..18023)
                     /locus_tag="VLD60_09425"
     CDS             complement(17859..18023)
                     /locus_tag="VLD60_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45220.1"
                     /translation="MKQRVTLTMEVDLIRKARQAVKAGAAPSLVSLVVEGLTRTVRRI
                     ERARGRRFWT"
     gene            complement(18118..18480)
                     /locus_tag="VLD60_09430"
     CDS             complement(18118..18480)
                     /locus_tag="VLD60_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45221.1"
                     /translation="MKWSTLIVALVLVIALASIATAGSAWVLWNEYVKFEGQSTGESS
                     VKFWDVINAFEDRQQCLAQAEKYMESVTTRYQNPSDAKLTTDEVGGRTRLRIHFHNHT
                     EIVSALCLPDTLDPREKK"
     gene            complement(18510..18719)
                     /locus_tag="VLD60_09435"
     CDS             complement(18510..18719)
                     /locus_tag="VLD60_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB45222.1"
                     /translation="MTNAITKRLPKRIKRLREERNLTQKRLANRSGVSHGYLARLEVG
                     MHDPSLNTLAKLAKALKVTVAELVK"
     gene            complement(18747..>19581)
                     /locus_tag="VLD60_09440"
     CDS             complement(18747..>19581)
                     /locus_tag="VLD60_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45223.1"
                     /translation="LLQVVAQGLFKVQTIARSIIERKWWWIRNGRICELLSSLKPWRI
                     VRPQGRRWNMLARGLSLLVFAAIAGCTTMPMPNIPNEPPLKNLALNGPIIVALSPFRI
                     KTSEGVRTYVSSYFSRRQSMRGWQAGQEYPIATQTVTEELRSLGITVVESTEDLLSPR
                     IREKLDFLLSGTVQPLRLNIRDTYSGNYTEGQYRLSLKVVDAQSGETVWERESNGQGS
                     LMNDPAVINDLAMSSNIVASLSQNEDRVSRLIITNAFRSLMRAHADEMRRIFTTKAGP
                     Q"
BASE COUNT         3934 a         5413 c         5698 g         4536 t
ORIGIN      
        1 tctaacttac tttttttctt tgtctacttc ttcgaactcg gcatccacaa ccttctcgtc
       61 ggtcttggcg ccattcggac tttctcctcc ggcggagtcg gctcccgcac ctgctgcagc
      121 agaggccgag gcttttttat acatctcttc agccaattta tgcgaggcgg ttgtcagtgt
      181 ctgggtggcc gactcgattg cttcggggtc attgccttcc atcgctttct tgagtgcagt
      241 cacggcctct tcgatcttgg ttttgtcatc ggccgcgatc ttgtcgccat gttccttgag
      301 attcttctcg gtctgataga tcaaattgtc agcctggttc ttcgcctcgg ccgctttgcg
      361 gcgtttcttg tcatcttccg tgtgggattg ggcatctcga acgagcttct cgacctcttc
      421 cttgctgagc ccgctggagg ccgtgatctt gatcgattgc tccttctgcg tggccaggtc
      481 tttggccgag acgtggacga tgccgttggc atcgatgtcg aataccacgt cgatctgggg
      541 catgccacgt ggtgcagggg gaaggccgac gagatcgaac tggccaagca atttgttgtc
      601 gttcgccatt tcgcgttcgc cttggaagac gcgaattgtc acagctgtct ggttatccgc
      661 tgcggtagaa aagatctgac tctttttggt tggaatcgtg gtgttccgct caatgagttt
      721 ggtgaacacg ccgccaaggg tttcgatacc caacgatagc ggggtcacat cgaggagaag
      781 cacgtctttg acctcgccct tgagcacgcc gccttgaatg ccggcgccga tggccaccac
      841 ttcgtcgggg ttcacaccgc gatgcggttc cttgccgaaa aactccttca cgacctgaat
      901 gaccttcggc atgcgtgtca tgccgccgac gagcaccact tcctggatgt ctcgcccgct
      961 cactccggca tcggccaggg ctttcttgca gggatcgatg gtgcgctgaa tgagatcatc
     1021 aaccagctgc tccagtttgg ccctggtcaa cttgatgacc atgtgtttgg ggccgctggc
     1081 atcagccgta atgaatggaa gattgatttc cgactcctgc gaggaggaca gctcgatctt
     1141 ggctcgctct gccgcttctt tcagccgctg aagggccatc cgatctttac gcagatcgat
     1201 cccctgatct ttcttgaact catcgacgag ccagtccatg acgcgttgat caaagtcgtc
     1261 gccgccgagg aacgtgtcac cgttggtgga ctttacctcg aacacaccct cgccgatttc
     1321 caggatagaa atgtcgaagg tgccgccgcc caggtcgtag acagcgatcc gctcatcttt
     1381 cttcttgtcg aggccatagg ccaacgacgc ggcggtcggt tcgttgatga tgcggagcac
     1441 gttcaatccg gcgatctgcc cagcgtcttt ggtcgcctgc cgctggctgt catcgaagta
     1501 ggctggtacc gtcacaaccg cttcggtcac tttttcgccg agatagtctt ctgccgtctg
     1561 ccgcattttt tgaaggatca ttgcggagat ttcggaagga ctataccgct tgccgcgcaa
     1621 ttctacgtgt gcgtcgccgt tgtcggcttc gaccaccttg tagggaagcc gtttcaaggc
     1681 ttcctggact tccttgctgc ggaattttct gcccatcagg cgcttgacgg aaaaaatggt
     1741 gttttccgga ttcgtaatcg cctggcgctt cgcaatttgg ccgacgagac gctcgttctt
     1801 gtccgtaatg ccgacaacgg acggcgtggt gcggctccct tccgcgttgg cgatgacaac
     1861 gggatctccg ccgctcatta tcgcgacgca ggagttggta gtacctaagt caatgccgat
     1921 cacttttccc atgggtgtat gctccttcct ccgaactaag attcccgcct ggtgttcaac
     1981 accatcagga ttcgttgggt gtgtcgttcg ttccgttcgc cgacgcggcg cccgttgata
     2041 cggccaccat ggctgctctc aagatgcgat cgtgcaaacg atagccccgt tgaaactctt
     2101 cgacgacgtg ttgctttgga acctcatctg aaggcacgga tgccacagct tggtgtccgg
     2161 acggatcaaa aggttgtccg accgtttgca tcggccggac gccgaatttc gttaagctcc
     2221 cgaccagctg tttcagtgtc agatcgacgc cctgcacgag agcctcgcca ttgccgccgt
     2281 ttttggcggc cttgattgcg cgctccagat tgtcgactac cggtaagagc tccttgagaa
     2341 gctgttcatt gccgaagcgg atctggtcgc gctggtcccg ttgagcgagc cgcttatagt
     2401 tatcgaactc ggcggccagc cgaaggtact tgtcgttgag agccttgagc tcctcggtct
     2461 tactcgttag agcctgctga atggcgagca cgtccgaaga aatctcgcca gtcgatccag
     2521 cctctaagtt atcgattgta ttggtaatct tttcttcttc tgccactggt ccatcctgtt
     2581 acggggagga gatagtcacc ctgcaagtga agtcaacggg ggccagaaaa attaattact
     2641 tatgaatgga ataggggagg tcatacccag tcagcagagg agagatttcc tgtagcccgc
     2701 cggtagagaa gtgccaagcc ctcgagtgtc agcattggct caatcttgtg gatggtggtt
     2761 gtctccggga cgatcactgt gctcaggcca ccggtggcga tgacataggc cgagcgaccg
     2821 gtttcctgtt cgattcgatg gaccaaggcg tctacgagtc cggcgtagcc gaacagcagc
     2881 cctgattgaa tgctgctggc cgtgtcgacc ccgatgacgg tcttgggttt cgtgagttcc
     2941 acttttgaaa gcttcgccgc gcgggcgaag agggcttcgg cagatattcc gagacctggt
     3001 gcgatcaccc cgcccaggta ttccccttcc ttggttatgg tgcagaacgt cgtcgccgtg
     3061 ccgaagtcta caacgatcaa gtcgcgccgg tacttatgat acgcagctgc tgcattcacc
     3121 aggcgatcgc tgccgatttc cttaggattc gcatagcgga gacggagccc cgtatctgtc
     3181 tctgtcgtga ccaccatcgg gcgttgatga aaatattgct ggatcaatgt ctcgaaggtt
     3241 gaggtgagtg cgggaacgac gctggacatg atggcggctg atatgtgaac cggcaggagg
     3301 cgggctgacg ccaagaggtt gattaagaga atcccgtatt cgtccgatgt cgttttggca
     3361 tccgtcgcca atcgccaatg ggccttcagc acggcgccgt cgtagacgcc cagaaccacg
     3421 ttcgtgttgc cgatgtcgat aacgagcagc atgcggccta ttctaaggct ttgcagggga
     3481 tttcgctagg ttctcaggtg gatgatgtcg gcgacgcgaa gatggacggt ttccggggat
     3541 tgcccctcct ccgggatggg gcgctggacg accgtgaggg aaccatcccg accgatcgat
     3601 tcggcgagac ccacaaactc cttcccttca gtcagcatgg ctttgactac cttcccgacc
     3661 gttctgcagc ggcgctgata ggccagcgcg atccgttccg agccatgggt agcaagttct
     3721 tcaacgcatg tctcaagttc gccgagtagt tgggcaagaa gtctattgcg gtcaacgctg
     3781 cggcccgttt catgggcgag agtcgtcgcc atgtctctca tgtccggcgg aaagtcggcc
     3841 aacgcaccgt tcacgttcag tccgatcccg acgacttgga acggttccga ttgtcgtgac
     3901 gttcccgttt cgcagaggat gcctccgact ttccgctccg caatgaccaa gtcgttcggc
     3961 catttgaccg ccacgttggc aaacgtgact gcttcgatgg cttcggctac agccagggag
     4021 gtgaccaacg gcagccatga aagccattcg gctagtcgct ccctaccgat cgtttttctc
     4081 aaaatgatcg agcaatagat gttgactccg ggaggggaaa accaactgcg tgagagccgt
     4141 ccgcgccctt tcgtctgagc ctcggcgagc acgatcgtgc cgtgctcaac gccaccttgc
     4201 gccagcgcga ctgcctcccg gtttgtcgaa ccgagctgcg catgcaattc aatgcggcgg
     4261 ccgagcgatt tggtcgccag cgtactctga atggcctgaa tgtcgagtgg tggagagggt
     4321 tgcattggct atggatgcct cgttcgactt tgagtgaacg gtgtcaactc caagctcatc
     4381 gtggcggcgg gagctgaatg agtcagcgcg ccgatcgaaa tgcgatcagg tcctgccgcc
     4441 gccatcgaac ggacattctt caaggtgatg ccgccggaga cttccaccaa tgcgcgtccc
     4501 ttgatcaatc gcacggcctt cttgaccatg gcgggcgtca tgttgtcgag caaaatgatg
     4561 tccgcgtggc cgttcaaggc ctgcttgacc tccgccaacg attccacttc gacgatcgtc
     4621 gagtgaccgg ctgtggcttt cgcgagacgg caagctgtaa gcacggccgg ctttttcttc
     4681 atcaacgcaa gatggttgtc tttgatcaag atgccgtcgc cgagggaacg gcggtgattc
     4741 gttcctcccc cgagtgcgac cgcccatttt tgcaacgatc tccagccggg aagggtcttt
     4801 ctggtgtcga ggacggcgac tggatagccg cgtaccgcct ggcaaaaccg ctgcgtcatg
     4861 gtggcgatgc ccgagagatg ttggaggaaa ttcagcgcca ctcgttcggc cataaggatg
     4921 gagcgcccat cgccatgaat ttccaaaagg gtgtcgccgt tcttcgcgtg cgcgccgtct
     4981 ttttgcacga caggaaaagt caacgaggga tcgacggtca caaacgtttg cagggccgcg
     5041 gccatgccgg ctatgacaag cggctgttgc gcgatgatgc gggcgcgggc ggggattcgg
     5101 attttgaaga gcgccgacgt ggtcagatcg ccttggccga ggtcttcttc tagcccctga
     5161 cggaccgcgc gtcggatgtc agcggccgga agcctgacca tgtcacgacg ttcctaacat
     5221 ggcgcgtagc tgttgctcgc tgctggccag catcgtttga tcatgttgca ggctatgcag
     5281 gcgagtctga tgatcgagaa tgacgtcggg cggtgctttg gcgacgaatt cctggttccc
     5341 gagtttgcct ttcagtcggc ccatttcttt ctcctgctcg tcctgctgtt tgacgacgcg
     5401 gtcgagcgcc ttcttgaggt cgacgtcgcc tgagacgaca agcccgactg aaagcccttc
     5461 ggtgacgagg cggagcaatt ttacagtcgg ccaatcggtg cgcggcgcga gctcggctgt
     5521 gccgcggccc agatgggcaa ggttgcgctg caattggtgc agccggctgt tacgagtcgg
     5581 atcgtcgtgc gacatgtaga aggaaatggg ctgtcccggc ggatagttta agagtacgcg
     5641 gccggtgcgg acgagaccga ccgtcttttc cagcagcgtg aagagttcct cggcctcggg
     5701 tgcccgccac gtctcgtccg gtgcgggata ggactgaatg acgatgctgt ctccctgatg
     5761 cggaattgtt tgccagatct cttcggtgag gaagggcatg aaggggtgca gcagccgcat
     5821 cgtggtttcc agcgtctcga cgagcgtctg cctggcacca gggccgtcgg ggtgcgccgc
     5881 atcttggagc gccggtttga tcaattccag ataccaatcg cagtactcgt gccagatgaa
     5941 atggtagagg acgctggccg cacgatcgaa tcgataggcc tcaagttccg aggtgatggt
     6001 ccggatcgta tggttgaggc ggctcagaat ccatcggtcg ggaaaggaac gtgctgttga
     6061 ggggtgggcg gctttgggac catcgaggta catcaagctg aaacgggcgg cattccaaat
     6121 cttgttggcg aaattgcgat agccttcgat ccgttcttcg gcgagcttga tgtcccgccc
     6181 cggcgaggcc atcgaagcca gcgtgaagcg cagcgcatcg gtcccgaatt gctccattac
     6241 atggagcggg tcgatcacgt tccctttcga cttgctcatc ttctgccctt cggcatcgcg
     6301 gacgagcgca tggatgtaaa cgtcgcggaa agggacgtcc tccatgaact tgaggccgag
     6361 catgatcatc cgtgcgaccc agaagaacag aatatcgagg cccgtgacga gcaccgatgt
     6421 cggatagtac ctcttcagct ccggcgtctg ttcaggccat cccaacgtgg agaagggcca
     6481 aagccctgag gagaaccagg tatcgaggac atcgggatcg cgcagaaaat tctgcccacc
     6541 gcaggtcgaa catcgtgttg gcgccgatcg ggccactatg ggtttcgcgc cctggagaag
     6601 agtgagacgg acgaccggta acccgtcatt ggatgttgtg acctgtttat ggatcaacgt
     6661 gctgttgcag gtttcacaat accaagcagg gatttggtgt ccccaccaga tctgccgtga
     6721 aatacaccag tctttgatat cgcgcatcca ccccagatag ttgttggtcc agccttcagg
     6781 aataatccgg atgcgcccat cctctactgc cttgatcgcc ggatcggcca gcggttgaat
     6841 cttgacgaac cactgagggg agaggtacgg ctcgaccacc gttttgcagc ggtagcattt
     6901 accgagcgcc atcttatggt cctcaatctt gacgagtgcg cccaggttgc gaaggaggcc
     6961 ttcaaccttg ggccgggctt ttgtgacagg gagaccacga agggcgtcga tcactgcatg
     7021 gtcgacggac gcgtcacgta atttgtctgc atcgagcaag gcttgatgat caaaaatagc
     7081 cagacgtgga agcctgtgcc gttcgcctgc ttcaaagtcg ttgaagtcat gcgccggagt
     7141 gatcttgacc gcaccggttc caaactcgcg atcgacaagg atggcatcac caacaatggg
     7201 aatcgcacga ttggtcagcg ggaggcgaac gcgttttccg attaaatgct tgaagcgatc
     7261 gtcctccgga tgcacggcaa cggcggtatc gccgagcatc gtttcgggtc ttgtcgtcgc
     7321 gacggtcagt tttatagcgg gatcctcttc cagcgggtac tcgatggagt agagcttacc
     7381 tttgatgtcc tcgtgctcca cctcgatgtc cgacaacgcg gttagacagc gtggacacca
     7441 gttgatcaac cgctcgccgc gataaatcaa tccatcttcg tacagccgga cgaacacttc
     7501 caagaccgct ttggagagcc cctcgtccat cgtaaagcgg aggcgttccc aatcgcagga
     7561 ttcgccgagt tgtttctgct gagtgacgat ggtattgccc gaggtcgctt tccattgcca
     7621 gacccgttcg atgaaccgtt cgcgtcccaa cgattcgcgc gaggtccctt cagccatcag
     7681 ttgcttctcc accacgtttt gcgtggcgat tcctgcgtga tcggtgccgg gcagccaaag
     7741 ggtattgcgg ccctgcatgc gccgccaacg gatgagaatg tcttgcaagg aatggttaag
     7801 cgcgtggccg acgtggagcg aacccgtcac gttcggcggt ggaatgacga tgcaataggg
     7861 ctgccccgga tgcgtcgtgg aggcgtgaaa gtatcctcgc tcaatccagg ttgcatacca
     7921 gcgggcttcg accgctttgg ggtcgtaggt tttgtcgagt tgtggagtgc tcatgctttg
     7981 gctcaagaaa aatgcggggc atcttaacat gcagggagta gcccctcaag gcgatgacgg
     8041 ctgttgtctg acggcttgag gggtgagggg gcttgtgcta tacagagctt cacgatgcga
     8101 gcgcagctcg cgtgaatggt cacactattt tggggagagg tacatggcaa cagggcgcga
     8161 aaaggatcgc aagacgagaa agaagcatcg gaagaatgtg caacggatga aagcgttggc
     8221 gaaggctcgg cgggggaaga agggcaaaag aggataacgt cgcgcgcagt ggcgccgcta
     8281 tgaactctcg gtgaggcgtg tgatctccgc cttgatctgc tcttcgatga tcgcgggaac
     8341 caatttctgg acggcctgtt gaatcttttc atccgcggcg gcgcggacga tgtctccagc
     8401 aagtttttcg gcctgtgccg tcgccgcctg tttgatttct tcctgaacct gcccctcgat
     8461 agatttcaga tgctcgcgga cggcctccgg gagcgaagcc ttcaccgcat cggcctgttc
     8521 gcggaccatc tgatccacgt tgtccttaat tagcgggctg gcaatctcaa agatgctgtg
     8581 acggatgatc ggttcgaggg tggccatctc gctgatcagt actttaggaa gttccttggc
     8641 caggagcggc tcgatcacct gcgtcaggcg ctcttgcgac aaaccggcgc cgagttgttg
     8701 attaacctcg gtttcaacgg ctttggcgac gagtcccgcg agttccttgg tgaccatctg
     8761 tggaaggaga tccgtgagct ttttctcggt tctctcggac atggattgca gcagctgacc
     8821 gaacaggcct ttcatggctt cttccggttc tgccgttgcc tgtttggccg tcggtgtcga
     8881 gggcatggga gtttctttct tatccgtgac cttggtttgc acggggaagg gaattgtgag
     8941 atttatgtcc tcatccccga cctgctcttc atcggctagt ccgtccggtg attcgtcatc
     9001 gtcgtaatcg gtcgcgcttg acgtcggggg ccaggatcgc ttcttcttgc cgccgttcgt
     9061 cgaggccttg gctcgatcga gatctttgat gatgtccaga aggttttccg actgaaaggg
     9121 tttcttcaga aacgccttga cgccgaggga tttgagatgg gattcatcga acttatcggt
     9181 tgccccggtc agcgcgacga tataggtgtc ggtcaggttg tccaatttat ggacttcctt
     9241 gcagaatcct gaaaatgtca tgttgtcgag atggtaatct gtgatgatga gattggggct
     9301 gagtttgcgg gccgcttcca gggcagcggg gccgtcttga aatccgacga cgtcataacc
     9361 ggccggtgtc gatatctgtt caaccatccg gcgtacggcg gggctactgt cgatgacgaa
     9421 gatggtggat gccacgcaac cctccaattt tattgactgg ttcgaagcta gcaggggggg
     9481 tattttctgt caagaaaacg cgcgatatgt accggttaat ttaaggatgt tccggtgata
     9541 acaatttgtg agccaggcat aaggatgtac gggcaatgag ccagccgatt caggtcgtat
     9601 ttttcgatgc ggccaataca ctctttcatg tcaacgggtc tgttgcggat atctatcttc
     9661 agcatgcggt gcagtttgga ttccagcaaa cgccggattc acgagcggcg atcacgcagg
     9721 ccttcagccg ggccttcaac gaggcgccgc ctccgatttt tgctgcaacc gaaccggctc
     9781 acatcaaaca gagcgaacgg ctctggtggt tcgatgtggt ccacaacgtg ttttatcggg
     9841 tgggcatgtt cgagcgattc gacgattttt tcgagcgggt gtttgaggtg ttcgaagatc
     9901 ccggatcatg gaagctcttt ccggaaaccg agcagacgct ggcggggctt cgcaagcaag
     9961 gaatggaatt gggggtcatt tccaacttcg attctcgtct tttttcggtg atgcgcgggc
    10021 tggggattca cgagttgttc gatacggtga ccatctccag tatggcccat gcggcgaaac
    10081 cggcgcccaa gatttttcag ctcgcgctgg acaagcacgc agtcgatccg gatgaagccc
    10141 tgcatatcgg cgacagtgtg cgggatgatg tggagggcgc cgtgaacgcc gggcttcacg
    10201 ccctgttgct tgatcgccag ggcaggcacc agggttcggg agtccgggtg attcggacct
    10261 tggatgaact gcttcccgtt atcgagcgct tatagcagca acggcaccac atctttggct
    10321 cttccctgga gggagaaatc aaccagctcg gagtgagggg tcggttccag gttgatttcc
    10381 gcgacgaagg ctccagcctc ttttgccact gacgcaaacc ctgccgccgg atacacgacg
    10441 ccggaggtgc cgatgacgag caggatctcg cacgtttgaa gaaccgccgc acaccgggcc
    10501 aggtcatcag ggaacaagga ttcgccgaac cagacgatgt ggggacgtag gagtccccgg
    10561 cagtgcggac aggtcgggag cagcgaaatc ggcatgtcgc ggttttcact gatcgatccg
    10621 caggccgtac aacgcaccat ccagatgttg ccgtgaattt cagaaagctt ttgcgatccg
    10681 gcggcacggt gcaatccatc gacgttttgc gtaatcaacc agaatgaccg gatgcgccgt
    10741 tccagttcga cgacggcatg atgggccgga tttggttgcg tgccggcgat acgttcgcgt
    10801 cgccagttgt accattccca gaccagtcgc ggatcacgct caaaagcctc gggcgtcgcc
    10861 aaatcttccg ctcgatactg acgccataag ccctccgtac ctcgaaacgt cggcacgccg
    10921 ctgtctgccg aaatgccggc tccggtcaag attgtgaggg accgtgcctt tgcgatgtgc
    10981 tctctcagga tcgcaagatc ggagcctggt gccatagctg tcagtattcg gtatagggga
    11041 tggagaagcg ctgcctccgg atattctgcc atgctatagt acgcctgttt ttggcgcaac
    11101 taatgaggga cggaatgaag cagattctga tggtgggagc cggatcggtg gggggattct
    11161 ttggcgccca cttggcgaag agcaactcca acgtgtcttt cctcttgcgg cctaagaccc
    11221 tcgccgccgt gcagcaacat ggcctgacga tccggagcgc tgccggaaca tttactgtgc
    11281 gaccgcgggc ggcatcggat gcgcgacaac tccccaggcc agacttgatc attcttggcg
    11341 tgaaggccta cgacctcgat gaagtgatgg cacaagtcga gccggtgctc tccgataaga
    11401 cggtcattct gacactgcaa aacggaattg atacggaaga tcgcatcatc gcgcgcctca
    11461 agcgagattg tgtggtcgga ggcgtggcgt tcatctattc caagattgcc gctccggggg
    11521 tcatcgatca ttacaagaaa ggcgctgtgg ccatcggtga gttgatgggc catgagagtg
    11581 aacggctctt acagattcga gatatctttg ccgcagcagg cattccctgc cagatatcga
    11641 aagatatccg tcgcagcaaa tgggaaaaga tgtgttggaa ttgtgttttc aatccgatca
    11701 ccgtcttgat cgatgacaag gtttccaagg cgctggacca tccggagatg atgggggtga
    11761 ttcggcaaat cgtcggggag atcaccgccg tgtcggctgc gttgaaagtc ccgttgccgg
    11821 cagatatgcc ggaacgggtg gtgaagtgga cgcaggaaat ccgggacatc catacgtcga
    11881 tgtatgatga ctggaaagct ggacggcaga cggaaatccg caacctgaac ggctacattg
    11941 ttgaacaggg ccgggcgctt ggcattccga cgccggtgaa cgaagcgctc acagccatga
    12001 tcaaaacgat gaccgaaatg gaaaaaaccg gatcaggtgt cgtgcggatc gacggcgctg
    12061 tcgtgcaacc ggtgtcgctg gatcgaacgg cgatgcgaca gcttccgggc gaacaccagg
    12121 ttgcggatgt cagtacggtc atgccgagta tgagagggcg tgcgatcacc ctgaagggct
    12181 tgctcgacgt gcccgcgctc ggcatccaag ccgaccatgt gacgtttcac tccagcgatg
    12241 ggaaatacgc cgcgacgttg acgctgcagc aggcgaagga cttcgggatg ctgctctatg
    12301 aactcgacgg ggagccgttg ccggctggga agggaggacc gtttcgcttg attacccccg
    12361 ggctcggcga tctctgtgcg aatgtcaaag gagtcgggcg gattgaagtg cgcgtcggat
    12421 cgggaaaaga caccagacca tccgagcgcc cgccggaatg tactgctgac gcacagtctt
    12481 cctaacctta gcggattacc tgaatggctt tccaccgttc cgaacgatag cgccaaagca
    12541 tcagcgccat cctcagtgac cagtccgcaa tcatcgccag ccacacatag aacactccga
    12601 gttcgaacca cacgccggtg ataaaagcga tcgggacacg catggcccac atgccgattg
    12661 ttgtggcgac cataatgaag tgggtatcgc ctgcgccccg gagcgagccg gccagtacca
    12721 tcgtgagggc aaggggaatt tggagcagcg ccacaatttt caggaacact gtgccgaggt
    12781 cgatgaccgc ttcgtcgctg gtaaacgcgc ggagcagtgc atagggaaaa aagaagaaga
    12841 ccacgcccat cgcggacatc acgatcgtag ccatgcgatt ggcttcccag ttttcgagtt
    12901 tggcacgggt gtatttcccc gctccgatgc tctgcccgac cattgtggca gcggcgatgg
    12961 cgaagccata gccgggcaga aacgacaagg attcgatcga gaggccgact tggtgagcgg
    13021 cgtacgacac cgtcccgtat aacagcacga tcttggtata gagcaggatg cccgcttgct
    13081 gcacgatccg ctcgccggaa accggtgctc ccacctgcca catcgttcgg aagaggtcga
    13141 accggacgtg tgtcgaccgg tgcaataagg gacggcaacg ctgaaacaga tagagcgcgc
    13201 cggttgcttc tgcaaccccg accgcaatgg ccgccccctg cacgccaacg gcgggcaatc
    13261 cccaccggcc gtagatcagt ggataggcga tcgcaatgtg caatatattg acgacaatca
    13321 tcgcgtacat cggtgtcttg gtatcgccgg tgccttgaag gattgacgag agcacctgga
    13381 tcaggacggt gaagggaatg acgagaaaaa tcagcgtcga ataggggtgt gcgagtgcca
    13441 tgacggcaga ttccgctccg agttgttgca tggcgaatcg attcaggagc atgccgctta
    13501 gcatgagaat gagcgaaacg gcgatggaca ggccgagaaa gtgccgtgcc gcttggcctg
    13561 cttcacgatg gcggcgcgcc ccccacaatt gcgcgacaat gacgtttgag ccgacagcaa
    13621 ggccggagac cagcgtggta gcgataaagg ccagtaattg gccgagcccg accgctgcga
    13681 tcggaatggc accgagtcca ccgacgagga agacggcgac gatcccttcc gttcgttgca
    13741 ggaggctgct gaccgttacc ggaagcgcca aggtcaggac ggagcgtcgg atctgggtga
    13801 caccgttggc catggggcgc tatcctagca gagttccgta gagtggcagg cagacatatc
    13861 cttttgcgtt tcccatagcg gttaaccatg acagagcttc acgatctctc accagctcct
    13921 gcgtccacgc ccccgcgttt gtcgaaatcc aaatttcttt ccgggctcca gtgccataaa
    13981 cgcctgcatc ttgaaatcca tcagccacaa ctcgcgactc cgccagatcc cggtacgcaa
    14041 gcgattttgg acatgggaac ggagatcggt gaattggccc gcaatcgatt tcccggaggc
    14101 gtgctggtga ccgccggata tcggcagcga gacgaggcgc tggcccggac ggcggcgtta
    14161 cttcaggacc cgaaggtgcc tgcgattttt gaggcggcgt tgcaacatga cggcgtgttg
    14221 gtgcgggttg atattcttga acgtgtgccg gcaggagcag gcgaaccgcc ggcctggcgc
    14281 ctgatcgagg tgaagtcttc aacaagagtc aaagatgtgc atgtggatga tctcgccatc
    14341 cagagtcagg tgctgtcggg agccgggctg gcactggccg gcacctatct catgcacatt
    14401 gatacgagct acctttacga agggggagag gtcgatctgg agacgctctt cgccatagag
    14461 gatctctcgg aggtggtcgc aggccgacgg acccacgtga cggagcaact tgccgcgatg
    14521 aaagccatgg tcctcctccc agacccaccg cccattgaac cggatcacca ttgtcatagc
    14581 ccgtacgtct gtcccttctg ggcccactgc acgaaagaaa agcccgagcg ttggatttat
    14641 cacttgcccg gatcgaagca gacggtcagc cgattggtgc agcaagggat cacgacaatc
    14701 gacgagattc ctgatactgc gaatctctcg cttgctcaac ggcgggtaaa agacaatgtc
    14761 gaatgggtct cgccaaaact gggatcatta cttcggtccg ttcagtatcc cgtgcatcac
    14821 gtggactttg aaacggtgat gctggctgtg ccgcgatttg ctacgacaag accataccaa
    14881 tccattccag tgcaatggtc caaccatatc gaactggaat ccggtgaact tctgcatcag
    14941 gaatttctcc acggcgatcc gacggagcct cgtcggcgat gggcggaagc attgatcgaa
    15001 tcactgggcg agaagggcag catcgtggtc tattccgcgt acgaagaggc gatgatcaaa
    15061 caactggtcg aggccttccc ggaatttcga acggcgttta atccgattat gaaacgattg
    15121 tgggatctgc acccaattat caaggatcac tattaccatc cgtcctttca ggggtcgtac
    15181 tcgatcaaat cggtattgcc ggccgtggtg ccgtcgatgg ggtacgagga tctggcgatc
    15241 aaagaagggg gacaggcggc gagcgaatac taccgaatgg tgtttgtcga gactgattgg
    15301 atcgaacagt cgacgatcaa ggaagcgctt ctccggtatt gtgcgcgaga caccctggca
    15361 atggttgaat tgcggagggt gctgggggag aaggccaacg gaggtggacg agagagctct
    15421 tgagaaggtc tatcccgttt gaagggcaag cactggagga gtgtatccac ccgcgccaat
    15481 tcgatacaac gagaaagggc gagcgggtca cacgaagtgc ggtagggcca tagagaatag
    15541 ttagtggtgc tttatcccgc cgccaagatc tactagagag gctccgggtc gagcaagctc
    15601 tgtacggcgg agagggtggg tattcgaagt cgcgttcgca ggggttgctc tcgctcctac
    15661 tgaggaggcc agctccctcg tagactcccc atgaaggggg cgaaccccct tcaggtcccc
    15721 ctgttcgaat cccacccagc gctgaaggtg ttttacccgg tcgcccagag ccatcacatg
    15781 gcgcccggta cgagcgagcg tgggatggcg gagagggtgg gattcgaacc cacggtcgag
    15841 ttaccccgac agcagttttc gagactgccc gattcggccg ctctcgcacc tctccgttct
    15901 cgaaaagccc gcgcataatc acacagttac ccgccgttct gcaagttcaa cgacatggcc
    15961 agaaaccggg ctgttgtgaa tagcgttgtg aaatgattcg gcgatcgttt cgatagcctt
    16021 cgccctgtgc ttcgcgctga gatgcgcata gcgttgaacg agagagatcg gcccatcccc
    16081 cgtattgctg caccgtgagt aggtccacgc tggccataac gagattggag gcgaacgtgt
    16141 gtcggagcgt atgaagggaa acccctgtac ctgataatcc tgcggcctga cacgccttca
    16201 agaagggttt ctcaatcgac ttgtagggga ggccgttggg tttgctgaag acgtactcac
    16261 tccgactgat tttcttgagc tggtgaaacg ccgctttggc tcggctgttc aacggtatgt
    16321 ttctcgtctg tccgttcttg gcataggcgg cttgcaccgt gaggaggccg cgggcgagat
    16381 ccacgtccga tcattttagg gtgagcgcct cgggccgcga tccgaatacc ggtgtttgtg
    16441 ccaatgataa tgagactgcg caagggttct ggcgagacct ccagtaactt cggctctccg
    16501 ttggcatcga agtagcgaag gcgccgcttc ggctcttcct gcatcttgac gccgagcgcc
    16561 ggattctcgc cttcatagtg tccccaggtc ttggctttat tcaccagatt cttgaggacg
    16621 gcaatttctc gattggcttg gatgcgagcg ccttgctcgg cccagtcccg cttatggcgc
    16681 tccacatcga acgacgagat ttgcccgagc gttttcccgc tgaatgagat cgctaagcgt
    16741 tcgagttgtt gccgatagac acgcaccgtt cgcggtcgtt tgtttgtgac ggtccaatcc
    16801 agaaaggcct ctttcgcttt ctcaatcgaa atgtccttgc gttttttgcc gataccggct
    16861 tctccacgga gaatagccgc gcgtttgacg ttggcgagtt cgccggccgt cgtccgctgg
    16921 atgttcttgc ctaacctgac cacatgccgc gccccattaa tgcggcaatc caggtaccac
    16981 atgatccctc tgagatagag tccatcacct cgtcgtgcca tgtccgcctc ccgttcttcg
    17041 tgtctgtatc cttaccaccg actacttggg tagaatgggc cgttctcctt caggtcctcc
    17101 tgatagaggg cgctcgcgtt gtcggggacg ctcagcgcca gccccaagcg ccgcgccacg
    17161 ttgtccttga gtaattcgat taaaacggga tcaaagggcg cgtagcggtc ctcgatgtcc
    17221 agattgatga tgtcgagttt cttggcgagt ttcgagaatc tcgaccggat gcactggacg
    17281 tggtagtcct cgaacacgat aacgtggccc gcccaacgaa ggtcctcctc cgtgatggac
    17341 cggcccgcca aaatcgggtt cgtgcccgcc gacctggctt cgcagcctgg cgtcgcgttg
    17401 agcatccccg ccaatgtttt ggaccggcat cgattccccg tgcacacggc caggactttc
    17461 gtcttttctg ttttctcttt cattcagtcc ttcggttgat cgccaatttt tcagctggcg
    17521 ggtttgaaga tcgcctgcgt cccgtccgca aaggtcagtt tgagcttgtg ttgcgtgacg
    17581 ccggtcccga cgggctccac cttcgcgatt gatccccgca tcttcttttt caagtccaac
    17641 ttgtcgatct tggctccttt ctgatcgttt tcaaattatt ccccagaggg cataatttct
    17701 ttttcggtgt gaccttcaag agttcccccg cccgcgtttt ccgccatgct ctccatcgga
    17761 cgcatctgag atcgggcctc atattcaaac tgtggcggtc gattctgcca tccctgctca
    17821 gagtttccct ttctccacat tggtgagttg gacgcgcatc atgtccagaa tcgtcgcccg
    17881 cgcgcccgtt caatccgtct gaccgtccgc gtcagtccct cgacgaccag cgacaccagc
    17941 gacggcgccg ctcctgcttt gacggcctgc ctcgccttgc gaataagatc aacttccatc
    18001 gtcagagtca cgcgctgttt catggcgtcc tcctgggagt gacaaggtat cgagtgaacg
    18061 atcacgacgg gtctcctgag gcgagcctcg ctcagctctc ctcgtcggtc attctgttca
    18121 cttcttctcc ctcggatcga gcgtgtcagg aaggcacagg gcagatacga tctctgtatg
    18181 gttgtgaaaa tgaatacgta atctcgtgcg acctcccacc tcgtctgttg tcagtttcgc
    18241 gtcgcttggg ttctgataac gagttgttac cgattccatg tatttctcgg cctgagcgag
    18301 gcattgctgc ctatcttcga acgcatttat cacatcccag aacttcactg agctttcccc
    18361 tgtggattgc ccttcaaact tcacgtattc attccacagc acccacgccg accccgctgt
    18421 cgcgatggat gctaaagcga tcaccagcac caaagccaca atcagcgttg accatttcat
    18481 ggcgacctcc gttctttcag cccgccctgt catttcacca actccgctac cgtgactttc
    18541 agcgccttcg ccagtttcgc caacgtgttc agcgaggggt cgtgcatccc gacctctaga
    18601 cgcgccaaat agccatgtga cactccggac cgatttgcaa ggcgtttctg cgttaggttg
    18661 cgctcttctc gaagtcgttt gatgcgcttg ggcagcctct tcgtaattgc gttcgttatt
    18721 acctataagg aatactaaag taaactctac tgaggtcctg ccttcgttgt gaatatacgt
    18781 ctcatctcgt ccgcatgcgc gcgcatcaag ctccgaaagg catttgtaat aataagccga
    18841 gaaacgcggt cctcattctg agaaagggat gcgactatat ttgagctcat cgcaagatcg
    18901 tttattactg cagggtcatt catcagtgaa ccttgtccgt tgctttctct ttcccataca
    18961 gtctctccgc tttgcgcatc gacaactttc aagctcagac gatactgccc ctcggtataa
    19021 ttgcctgaat aggtgtcacg gatgttgagg cgaaggggtt ggactgttcc agacaacagg
    19081 aaatcgagct tctctcttat cctaggcgac aataagtctt ccgttgactc aacaacagta
    19141 attcccaggc ttcgcaattc ctctgtgaca gtttgagtcg ctatcggata ttcttgccct
    19201 gcttgccatc cccgcatcga ttgccttcgg ctaaaatacg agctgacata tgtgcgcact
    19261 ccctcactcg tcttaatacg aaagggagaa agagctacaa ttatcggtcc attcaaagcg
    19321 aggtttttaa ggggcggctc gttgggaata ttaggcatcg gcatagttgt gcaaccagct
    19381 atagccgcga aaactaacaa cgatagccct ctcgccagca tattccacct cctgccttgt
    19441 ggccgcacga tacgccaagg ttttagggat gacaacagtt cacaaatacg tccatttctg
    19501 atccaccacc attttctctc aatgatgctt cgtgctatcg tctgtacctt aaataagccc
    19561 tgcgcgacca cctgaagaag t
//
LOCUS       DATIFR010000157         4065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1252747, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000157 DATIFR010000000
VERSION     DATIFR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252747"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..630)
                     /gene="pyrR"
                     /locus_tag="VLD60_09445"
     CDS             complement(70..630)
                     /gene="pyrR"
                     /locus_tag="VLD60_09445"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSB45224.1"
                     /translation="MKTPSQHDRQQERVVMESGDIARAVTRIAHEILERNKGVKDLAL
                     VGIRTGGVHLAHRLVRRIHDIEGVAVPIGELDITLYRDDLALRKEQPILRKTSVPFDI
                     SSKIIVLVDDVLFTGRTIRAAMDGLIDLGRPAEIQLAVLVDRGHRQLPIKATYIGKNI
                     PTSREEKIQVLLEEAGEEDRVVILKA"
     gene            complement(818..1504)
                     /locus_tag="VLD60_09450"
     CDS             complement(818..1504)
                     /locus_tag="VLD60_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45225.1"
                     /translation="MSEQVSGQTGDSDDKIVIYWEREYSTAFPPERLKLDFMPHRPML
                     SGMSLDEQRATAAGGYKVIPDDCGPVRTVGQYGWLIRSPADCKIRRTADGVKWQAPPI
                     LPEERLLGYKSFSGTYVDLILNSGYPKLCCGIRFYYPKHVGLMMKDIPNPFYHHPERT
                     FYVWEGIKTQEYKRTPNVYDWLPDYEAFTANFLLQLTKPTTIKRGDPIGIVIPVLLPK
                     QFSLEELKPR"
     gene            complement(1548..3332)
                     /locus_tag="VLD60_09455"
     CDS             complement(1548..3332)
                     /locus_tag="VLD60_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45226.1"
                     /translation="MSADPLAVGLKIPGSLRFPANLPFINQFEQSGGRVVRQSTGHLV
                     YYRSDGRRFLATDPAGNPLHECDWGLDAKGEATLLRARIKLDWGAWVGLKPAGLVNEM
                     SLNLATKPGWQKLKADDLRGMAAQALRVPLDEVRWFYRDEDLVIGPTGRATIRHRKDA
                     FYLLDDGDFDRARFMSCMGAMHWAAIDFLPVVELFKSLLPGTGSAVFELIRGLYDDQN
                     EGLPVPRPLRYRGIPTYPSEAAFRLFSSFFRPTAGGERDPFTIFMDPTRAHEVEWSPS
                     PIQPRRYFDGTHGCCLTVLGATLQKLTMTEDSAGLSFLNPKGRRFAPCDRTVQIIHGT
                     VVVKDRDRETALSVVPPKECQEQPPLSVPMSPVDWRSLFISGVPPIAPQEAYGAVLFY
                     PQNETEIGELAAQPFVADYLQDLAEQDREIGAAIARAERILIENGDAVIATCIPFDRP
                     RDYDVLVRVPSFAQRQAQQLWNTSAELQRWDWLKRIRFLPTGKAEDSSGSQSSYDLVY
                     HWVPYPLEGNHPALMARIQQLQKGVSRAGRAFVVGPADLGALWRTCGLAVCWQESVEQ
                     LPTFRMHRTILPQAQLRAGLTLFHLKRA"
     gene            complement(3333..>4065)
                     /locus_tag="VLD60_09460"
     CDS             complement(3333..>4065)
                     /locus_tag="VLD60_09460"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSB45227.1"
                     /translation="GAISFVTRPDRELVPVLLKKDQCIDLIIPRGGESLMRIVTEHST
                     IPVIKHDAGVCHIYVDVDADPVMAEEICFNAKVQRPSTCNAMETLLVHQGIARTWLPA
                     FIDKLVAAKVEVRGCQKTCALSSAAKPASDSDYGKEFLDLTLAVKVVKNIDEAMEHIT
                     RYGSRHTEAIVTADYQKAMRFLREVDASAVLVNASTRLNDGYQFGLGAEIGISTARIH
                     ARGPMGLEELTCSKFIVLGSGQVRE"
BASE COUNT          899 a         1199 c         1079 g          888 t
ORIGIN      
        1 atctcatccg ccgacaaggg cgctaagctc agaagatctt tgcgtttgag gctcatcgct
       61 tcgtctcctt cacgccttga gtatgacgac ccggtcttct tcgcctgcct cttccagcaa
      121 aacctggatc ttctcttccc gtgaggtcgg aatgttcttt ccgatatacg ttgctttgat
      181 cggcaactgc cggtggcccc gatcgaccag gaccgccaac tgaatttctg ccggccggcc
      241 gagatcgatt agtccgtcca tcgcggcgcg gatcgtccgt ccggtgaaca ggacgtcatc
      301 gaccagcacg ataatcttgc tcgagatgtc gaacggcacg gacgtcttgc gaagaatcgg
      361 ctgctctttt cgtaaggcca aatcatctcg atagagcgtg atgtccaact ctccgatagg
      421 aacagccacg ccctcgatgt catggatccg tcgcacaagc cggtgagcga gatggacgcc
      481 tcccgtcctg attcccacca atgcgagatc cttgacaccc ttatttcttt cgagaatttc
      541 gtgcgcgata cgtgtgaccg cacgggcgat gtcgcccgat tccatcacca ctcgttcctg
      601 ctgccggtca tgctgggaag gtgtcttcat ctggaatccg ggcataaaaa aacctccccg
      661 ccgagattcg acgagaaggt cacagtttgc tgtcgatgaa aggcctgaat gaggcccata
      721 ttcactcctt gctcacctct caggatgagc attaaaggtt gatgcatcat actgaggcgg
      781 gaggaaggct gtcaagcttg aagaggatcg tcgggcccta gcgaggcttc aactcttcca
      841 gcgagaattg tttgggcagg agtaccggta tgacaatgcc gatgggatca ccccgtttaa
      901 tcgtcgtcgg tttggtaagt tgaagcagaa aattggctgt aaaggcctca tagtccggca
      961 accagtcata gacattcggc gtgcgcttgt actcctgcgt cttgattcct tcccagacgt
     1021 agaaggtccg ttcaggatga tgatagaagg gattgggaat atccttcatc atcaagccga
     1081 catgtttggg gtaataaaac ctgatgccgc agcacaattt gggatagccg ctgttgagaa
     1141 tcagatcaac ataggtaccc gaaaaggact tgtaccccag caatcgctct tcgggtaaaa
     1201 tcggcggcgc ctgccacttc acgccatctg cggttcgccg gattttgcag tctgccgggg
     1261 accggatcaa ccatccatac tgcccgaccg tgcgcaccgg tccacaatca tcggggatca
     1321 ccttatagcc gccagcggcg gtggcccgtt gctcatcaag cgacatgcct gacagcatcg
     1381 gccggtgcgg catgaaatcg agtttgagac gctcaggcgg gaatgccgtg ctgtattcac
     1441 gttcccaata gatgacgatc ttgtcgtccg aatctccggt ctgtcctgac acctgttcac
     1501 tcattgttcg ccaccgtaat cgaaacgtca tccgccgtgc aagcccgtta cgctctcttg
     1561 agatgaaaca gcgtcagccc agccctgagt tgagcctgag gcaggatggt tcgatgcatg
     1621 cgaaacgtcg gtaattgttc aacgctctcc tgccaacaga cagcaagtcc gcacgttctc
     1681 cacaaagctc ccaagtcggc cggccctacg acaaacgccc ggcctgctcg gctcacccct
     1741 ttttgaagct gttgtatccg agccatcagg gcaggatgat tcccttccaa aggatagggc
     1801 acccaatgat agaccaaatc gtagctgctc tgtgaaccgg aagaatcttc agccttccct
     1861 gtcggcagaa acctgatgcg tttcagccaa tcccagcgct gcagctcagc actcgtgttc
     1921 cataattgct gggcctggcg ttgtgcaaac gacggcaccc gcaccaagac gtcatagtcc
     1981 cgcggccgat caaacggaat gcaggtagcg atgacagcat caccgttctc gatcaggatc
     2041 cgttcagccc ttgcgattgc agctccgatc tctcgatctt gctctgcaag gtcttggaga
     2101 tagtcagcca caaagggctg ggctgcgagt tccccgattt ctgtttcgtt ctgcggataa
     2161 aacagcaccg ctccatacgc ctcctgcggg gctatcggcg gcactccgct tataaacaga
     2221 cttcgccaat caacaggact catcggaacc gatagtggtg gttgctcctg gcattctttt
     2281 ggcgggacta ccgacaaggc agtttcacga tcacgatctt tgaccaccac cgttccatgg
     2341 atgatttgca ccgttcgatc gcaaggggca aatcgtcgac ctttcggatt gagaaaggat
     2401 aagccggctg aatcttccgt catggtcaat ttttggagcg ttgcccccag aacagtcagg
     2461 caacagccgt gtgtcccatc aaaataccgg cggggctgga tcggcgaagg agaccactcg
     2521 acttcatgtg ccctggttgg atccataaag atggtgaagg gatcgcgctc cccgcctgca
     2581 gtcggcctga agaagctgct aaacagacga aaggctgctt cagatggata ggtcgggatg
     2641 ccgcgatacc ggagaggtcg tgggaccggc aggccctcat tctgatcgtc gtaaagcccc
     2701 cggatcagct caaacaccgc cgaaccggtg ccggggagaa gcgacttgaa gagctcaaca
     2761 acgggcagga aatcaatggc agcccaatgc attgctccca tgcaagacat gaaacgggca
     2821 cggtcgaagt ctccgtcgtc cagtagatag aacgcatcct tgcgatgtcg aatcgtcgcc
     2881 cgtcctgtcg ggccgataac caagtcctcg tcccgataga accaccggac ctcatcgaga
     2941 ggaacgcgga gtgcttgcgc cgccatacct ctcaagtcgt cggccttgag cttctgccat
     3001 cccggcttgg tggccagatt gagactcatc tcattcacca agccggccgg cttcaggccg
     3061 acccacgcac cccaatccag cttgatgcga gcgcgtagca gggtcgcctc ccccttcgcg
     3121 tccagccccc agtcgcattc atgtaaggga ttcccggcag gatcggttgc gaggaaacgt
     3181 cgtccatcgg accggtaata gaccagatgg cccgtcgatt gtcgaacgac gcgccctccc
     3241 gattgttcga attgattgat gaagggaaga ttggcaggaa atcggagaga gccgggaatc
     3301 ttgaggccaa cggcgagggg atcggctgac atttactccc ggacttgccc gctccccagc
     3361 acaatgaact tggagcaggt caactcttca agccccatcg gtccacgcgc atggatgcga
     3421 gcggtgctga tcccgatttc agcaccaagc ccgaactggt agccatcgtt gagcctcgtg
     3481 gaggcattca ctaacacggc actcgcatca acttcgcgaa ggaaccgcat ggctttttga
     3541 tagtcggctg tcacgatggc ttccgtatgc cgggaaccgt accgcgtaat gtgctccatg
     3601 gcttcgtcga tgttcttgac cactttcacc gccaaggtga gatccagaaa ctctttcccg
     3661 tagtcgctgt cacttgccgg cttggccgct gacgacagcg cgcaggtctt ctgacagcca
     3721 cggacctcga ccttcgccgc taccaatttg tcgataaacg ctgggagcca cgttctcgcg
     3781 atgccttggt ggaccagcaa tgtctccatc gcattacagg tcgaaggtcg ctgaaccttc
     3841 gcgttgaagc aaatctcctc agccatcaca gggtcggcat cgacgtccac atagatatga
     3901 cagacaccgg catcgtgctt gattacggga atcgtcgaat gctccgtgac gatgcgcatg
     3961 agcgattcgc cgccacgtgg aataatcagg tcaatacatt gatccttctt caacagcacc
     4021 ggcaccagtt cacgatcagg tcgcgtcaca aagctgattg cgcca
//
LOCUS       DATIFR010000158         1323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1827075, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000158 DATIFR010000000
VERSION     DATIFR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827075"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..614
                     /locus_tag="VLD60_09465"
     CDS             264..614
                     /locus_tag="VLD60_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB45228.1"
                     /translation="MTRTYLHVVSGLMIVCWLAVPIAGLTQTERNLPRGKKLYEKYCL
                     GCHGPQGKGDGATQFDPPVADLTSSEVLTKPDSRLLKSIHEGRPNSAMDAWKSAMSEE
                     AIRDVLAYVLTFPR"
     gene            632..1108
                     /locus_tag="VLD60_09470"
     CDS             632..1108
                     /locus_tag="VLD60_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45229.1"
                     /translation="MKSSWKTGLSFGLTSGVITTLGLMVGLHSGTHSRAIVIGGILTI
                     AIADAMSDALGIHLAEESKNSGPTMQIWEATIATFFAKLIIALTFVVPVLVLPLTAAI
                     MVSVTWGLLLLAVLSFALARAQNIAPWKVIGEHVLIGLSVVGMTHVLGDWIRETLS"
     gene            1119..>1323
                     /locus_tag="VLD60_09475"
     CDS             1119..>1323
                     /locus_tag="VLD60_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45230.1"
                     /translation="MDQARTVNTPLVAYLSMEIGLNPAMPTYAGGLGVLAGDTIRSAA
                     DLDVPMVAVTLLHRRGYFYQRLDA"
BASE COUNT          279 a          349 c          421 g          274 t
ORIGIN      
        1 cgggtaaaca agttgcttgg agaaaggaaa gccgcgcttc aagaaggcgc gaagctgctg
       61 ttggaacgtg aagtcatcag cggggtggag ttgaaagcga tcatggagaa gacctgaagg
      121 cgtcagccgc aatggtctat atcaagaccg acagcatgat gcttaaccaa gcaggcgggt
      181 ggtttcgggg atatcttcga cgggctcacg aacgcggtcg gtgagccggc gcttcgagaa
      241 agtcgaacag gaaaggggaa cagatgactc gcacatacct gcatgtcgtc tccggactga
      301 tgatcgtctg ctggctggcg gttcccatcg cggggctcac tcagaccgaa cggaatctcc
      361 cgcgagggaa gaagttgtac gagaaatact gtttgggctg tcatgggccg cagggtaaag
      421 gggatggggc cacgcagttt gatccgccgg ttgcagatct gacgtcgagc gaggtgctca
      481 cgaagccgga ttcgcggctg ctcaagagca ttcacgaggg acggccgaac agcgccatgg
      541 atgcctggaa atcagccatg tcggaagagg ccatccggga cgtcctggcc tatgttctca
      601 cctttccgcg gtaagccgtg caagaggaag catgaaatcg tcctggaaaa ccgggctgag
      661 ttttggactg acgtccggcg tgatcaccac actcggtctt atggtgggat tgcactcggg
      721 gacgcactca cgtgcaatcg tgatcggtgg aattctcacc attgcgatcg ccgatgccat
      781 gtccgacgcg ctcggcatcc atctggccga agagtccaag aacagcggcc ccacgatgca
      841 gatctgggag gcgaccatcg cgacgttttt tgcgaagctg atcattgcac tgacctttgt
      901 cgttccggtg ctcgttctcc ccctgacagc tgcgatcatg gtgagtgtga cgtgggggct
      961 cttgctcctc gcggtcctca gttttgccct tgcacgcgcg cagaacatcg ccccatggaa
     1021 ggtgatcggt gaacatgtgt taatcggcct ttctgtggtg gggatgaccc acgtgctggg
     1081 agactggatt cgagaaacac taagctgaga gaggcgtcat ggatcaggca agaaccgtga
     1141 acactcctct ggttgcatac ctgtcgatgg aaatcgggtt gaatcctgcc atgccgacct
     1201 atgcaggagg gcttggcgtg ctggccggcg acacgatccg gtccgccgcc gatttggatg
     1261 tgcccatggt ggcggtcaca ctcctccacc ggcgcggtta tttctatcag cgcctcgatg
     1321 cgc
//
LOCUS       DATIFR010000159         6359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_679074, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000159 DATIFR010000000
VERSION     DATIFR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679074"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VLD60_09480"
     CDS             complement(<1..242)
                     /locus_tag="VLD60_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSB45231.1"
                     /translation="MATDVTMRAKDVLGVYQPGSTHMVGDGFPVRNLFPSNDLDHEVS
                     PFLMLDYAGPQYFAPTDHPRGVGEHPHRGFETVTIV"
     gene            complement(268..717)
                     /locus_tag="VLD60_09485"
     CDS             complement(268..717)
                     /locus_tag="VLD60_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB45232.1"
                     /translation="MKALFQTDDSWTGLILRLTLGMVMFPHGAQKLLGWYGGFGFSGT
                     IGFFTDTMHLPWLIAFLVIIGESFGSVALLLGFLTRFTAASLAVIMVGAIVTTHIPHG
                     FFMNWFGKQQGEGYEYHLLMIGIAVALLVTGAGRWSIDREIAERVAR"
     gene            complement(946..1413)
                     /gene="smpB"
                     /locus_tag="VLD60_09490"
     CDS             complement(946..1413)
                     /gene="smpB"
                     /locus_tag="VLD60_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSB45233.1"
                     /translation="MTKDVTEKAVATNRKAYHDYFIEEKFEAGIMLQGTEVKSLREGR
                     VNLQDSYASVKDGEVFLHHCHISPYSHGNIMNHDPLRVRKLLLHKKEINKLLGKTQQK
                     GLTLIPLRIYFSKRGHAKVELGLAKGKKLYDRRESIKSREAGREVERAIKERK"
     gene            1473..1946
                     /locus_tag="VLD60_09495"
     CDS             1473..1946
                     /locus_tag="VLD60_09495"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSB45234.1"
                     /translation="MRAHSSLDSFGTILSGLAKRLGLETRLFELRLQHQWREIVGDPV
                     AAHTWPDQIRFKKLHLIVRSSVWMQQLTFLKPALLAKLNEQAGTELVTEIALRVGEIP
                     SLDKPSGQAVPANTAPVVSEAALTEASAHAAAVQDLELRTRLTQVMAEAFARSRP"
     gene            complement(1943..2512)
                     /locus_tag="VLD60_09500"
     CDS             complement(1943..2512)
                     /locus_tag="VLD60_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45235.1"
                     /translation="MNDSYTQPPAAPKKKPLDTVVKLALMVFFGSFALIWGGMYLSRP
                     DRSIPPYSVGSQEDTAVSVHVPAWTSDTEIETLIERFRKVGRETRNFGPMKIRPTTPD
                     DPKGRYRRITIYIFTHDAWAEAPILHKYLTGEDKDVLDGFRRALRGFYQLTESQEEGR
                     IGPLVEGQDSAATAAYSRQLFKESVASTP"
     gene            complement(2559..5036)
                     /gene="gyrA"
                     /locus_tag="VLD60_09505"
     CDS             complement(2559..5036)
                     /gene="gyrA"
                     /locus_tag="VLD60_09505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSB45236.1"
                     /translation="MPPDERLGHIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILFGMNEMGLVHNRAYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQEFNMRYPLV
                     DGQGNYGSMDGDSAAAMRYTEARMTKLAEELLADIDKETVDFGPNYDESRQEPLVLPT
                     KVPNLLINGAGGIAVGYATNIPTHNLGEVVEGLLLLLENPEVTIAQLMKKIPGPDFPT
                     AGFIYGMTGIKDAYETGRGLLKLRAKVAVETDERTDRERLIVTEVPYQVNKAKLIEKI
                     AELIQEDRLKGISDLRDESSAREGVRIVIELKRGEIPLVVLNNLYKHTQLETTFGVIM
                     LALVNNRPEVLNLKQILHHFLEHRREVVVRRTAFELRKAEERAHILEGLKIALDNLDA
                     VIALIRRAQSPDVARAGLIQQFALSEIQANAILDMRLQRLTQLERTKLIEEYTEVLKQ
                     IEYLKSVLGSEALVRKIIKDELIQINEAYKDERRTQIVKEEAEISLEDLIAEEEVVVT
                     ISHAGYIKRNAASLYRAQRRGGKGKIGMGIKEEDFVETLFTASTHDTLLFFTDAGKVY
                     WLKVHEIPEASRAAKGKALVNLLALSGNEKVTATLPVKEYREDRFVIMATKKGIIKKT
                     ELAAYSNPRQGGIIALGLEDGDKLIGVHVTDGQREILLGTKQGITIRFKEEEVRPMGR
                     TAYGVKGITLEEGNEVIGMETITPDSTTSILTVTEGGYGKRTPVTEYRVQGRGGKGII
                     SVKTTERNGLAVGFLQVRDDDEIMLMAAQGKVLRCKVDDIREIGRNTQGVRILDLDGE
                     EDRVVAVAKLAESVEREDPGADESANG"
     gene            complement(5118..>6359)
                     /locus_tag="VLD60_09510"
     CDS             complement(5118..>6359)
                     /locus_tag="VLD60_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSB45237.1"
                     /translation="SLPGKLADCSEKDPALSELYIVEGDSAGGSAKQGRDRKFQAILP
                     LKGKILNVEKARFDKMLTSDEIRTLIMALGTGIGRKREESDKADKDTFDIAKARYHKI
                     ILMTDADVDGSHIRTLLLTFFFRQMPELLERGYIYIAQPPLFKVKKGKSERYLKDEGA
                     LNEHLADLAVEEVELYVEGAQDYVTGRRLLPILKKMISFETLLTRLDKKSHEAAMLRV
                     FVDEPGLDRDMLKDLAALKKTVANAKKALGLVFPKLEPTIEIFPDEEHQSNKVSCRMH
                     AGGITHQLDVTHELVGSAEFRELQKLAPSSIGLGRLPYKLKAKGQEQQLHSTADMVKT
                     ILDLGKQGLGIQRYKGLGEMNPEQLWETTMNPEMRTLLKVELEDVTGVDEIFTILMGD
                     EVEPRRNFIQAHALEVRNLDV"
BASE COUNT         1210 a         1904 c         1734 g         1511 t
ORIGIN      
        1 tagacaatag taacggtttc aaacccgcgg tgcggatgct cacccacgcc gcgaggatga
       61 tctgtcgggg caaagtactg gggtcccgca taatcgagca tgagaaaggg gctcacctca
      121 tgatcgagat cattgctcgg aaagagattc cgaacgggaa acccgtcgcc gaccatgtgg
      181 gtcgaccctg gctgatagac acccaggaca tccttcgctc tcatcgtcac atccgttgcc
      241 atgattgccc tccttccctg cgcgatctta gcgcgcgact ctctctgcaa tctcgcggtc
      301 gatcgaccac cgcccagctc ccgtcactaa caacgcgacc gcgattccga tcatcagaag
      361 atgatactcg taaccttcac cctgttgctt gccgaaccag ttcatgaaga acccgtgggg
      421 aatgtgcgtc gtcacgatgg cacccaccat aatgacagcc aggctggccg ccgtaaaccg
      481 tgtaagaaac cccagcagca gggcgacgct tccaaatgat tctccgatga tgacaaggaa
      541 tgcaatcagc cacgggaggt gcatcgtgtc cgtaaagaaa ccaatcgttc ctgaaaaccc
      601 aaacccgcca taccagccca agagtttctg ggcgccgtga ggaaacatca ccatacccag
      661 ggtcaggcga agaatcaggc cagtccaact atcgtccgtt tggaacaacg ctttcatacg
      721 gcacctccgc ttgtctgatc accattcaaa caactgagtg caagatagtt ctagttagaa
      781 ctaaagtcaa ggtctccctt gcctaggacg gtcgtcattg cctttcttct tattaatcag
      841 atgcttggat caatagcgca tggccgaatt gaaggggcag gtccctgccg tctggcgtgg
      901 catcgacgaa tcctcaatgg gatcacattc ccaggatccg gtgtcttact ttctttcttt
      961 gatcgcccgc tcgacttcgc ggccggcttc gcgcgacttg atggactcgc ggcggtcgta
     1021 aagcttcttc cctttggcga gtcccaactc gactttggca tgacctcgct tggagaagta
     1081 gatgcggagg ggaatgaggg tgaggccttt ctgctgagtc ttgccgagca gtttgttgat
     1141 ctctttttta tgcagcagga gcttgcggac ccggagcggg tcgtggttca tgatgttgcc
     1201 gtggctgtag gggctgatgt ggcagtggtg gagaaagacc tcgccgtcct tcacgcttgc
     1261 atagctatct tggagattca cccgcccttc ccgaagagac ttcacctcag tcccttgcag
     1321 catgatgccg gcttcaaact tctcttcgat aaaataatcg tgataggcct tgcgattggt
     1381 cgcaacagcc ttctctgtga cgtctttcgt catcgccgtc cttggctcct aggctcttga
     1441 ccctttgaaa gtctgggcct aagatcctgt ctatgcgcgc gcatagttcg cttgactcat
     1501 tcggtaccat tctctcggga ttggcgaaac gtcttgggct cgaaacgcgc ctgttcgaac
     1561 tccgtctcca gcatcaatgg cgtgagatcg tcggcgatcc cgtcgccgcc catacctggc
     1621 ctgaccagat ccgcttcaag aaactccatc tgatcgtccg gagctcggtg tggatgcaac
     1681 agctgacatt cctcaaaccg gctttgcttg ccaagctcaa tgagcaagca gggaccgaac
     1741 tcgtcaccga gatcgccttg cgtgtgggag aaattccctc gctcgacaag ccgtctgggc
     1801 aagcggtccc ggcgaacaca gcaccggtcg tgagcgaggc ggcgttgacc gaagcctctg
     1861 cgcatgcggc cgccgttcaa gaccttgaac tcagaactcg tttgacccaa gtgatggccg
     1921 aggccttcgc tcggtctcga ccctaaggcg tcgaagcgac cgattctttg aacaactgtc
     1981 tggaatacgc cgcggtcgct gcagagtcct gcccctcgac gagcgggccg atccgccctt
     2041 cttcctgcga ttccgtcaat tgataaaagc ctctgagcgc acgccggaac ccgtcaagga
     2101 cgtctttgtc ctcgccggtg agatacttgt ggagaatagg tgcttcggcc caggcatcat
     2161 gagtaaagat atagatagta atacgccgat agcgtccctt gggatcgtcc ggtgtggtcg
     2221 gcctgatctt catcgggcca aaatttctgg tctcacgacc gacttttcga aagcgttcga
     2281 tgagtgtttc tatttccgtg tcgctggtcc aggccggcac atgcaccgac acggcggtgt
     2341 cctcctgcga accgacactg tacggaggga tcgatcgatc cggtcgactg aggtacatcc
     2401 cgccccagat cagggcgaag gatccaaaaa acaccatcag cgccagtttc acgacggtgt
     2461 cgagcggctt cttcttggga gcagccggcg gctgagtgta tgaatcgttc atggcgggaa
     2521 gaggagcgaa gggttccgcg tcctgacggg gagtggccct agccgttggc gctttcgtcg
     2581 gcacccggat cttcgcgctc gacgctttcg gcgagcttgg cgacggccac cacacgatcc
     2641 tcctctccgt cgaggtcgag aatcctgacg ccctgcgtat tacgtccgat ctcgcggata
     2701 tcgtcgactt tgcagcgaag gaccttcccc tgtgcggcca tgagcatgat ctcatcgtcg
     2761 tcccgtactt ggaggaatcc gacggcaagg ccgttccgtt cagtcgtttt tacactgatg
     2821 atgccttttc ccccacgccc ctgaacgcga tactccgtga ccggcgtccg cttgccgtat
     2881 cccccctcgg tcacggtgag aatcgaggtg gtggagtccg gtgtgatcgt ctccatcccg
     2941 atgacttcgt tgccctcctc gagggtaata cctttcaccc cgtacgccgt tcgacccatg
     3001 ggcctgactt cttcctcttt aaaccgaatc gtaatgccct gtttggtccc aagaagaatc
     3061 tcgcgctggc cgtccgtcac gtgcacaccg atgagtttgt cgccatcttc gagccccaac
     3121 gcaatgattc cgccctggcg cggattgctg taggccgcga gctcggtctt cttgatgatc
     3181 cccttctttg tcgccatgat cacgaaccgg tcttctcgat actccttcac cggcaacgtc
     3241 gccgtgacct tctcgttgcc ggacaaggcg agcagattca ccagcgcctt tcctttcgcc
     3301 gcgcggctgg cttccggaat ctcgtggacc ttgagccaat acaccttccc tgcatcggta
     3361 aagaacagaa gcgtgtcgtg cgtcgaggcg gtgaagagcg tctcgacgaa atcttcctcc
     3421 ttgatgccca tgccgatctt gcccttgccg ccgcgtcgct gcgcgcgata gagcgatgcc
     3481 gcgttgcgtt tgatgtagcc ggcgtgcgaa atcgtgacga cgacctcctc ttccgcaatc
     3541 aagtcctcaa ggctgatttc ggcctcttct ttgacgatct gggttcggcg ctcgtccttg
     3601 tacgcctcgt tgatttgaat cagttcgtct ttgatgatct tccggaccag cgcctcgctg
     3661 cccaatacgg atttcaaata ttcgatctgt ttcaggactt ccgtgtactc ctcgatcagt
     3721 ttcgtccgct ccaattgggt caagcgctgg agacgcatgt ccagaatcgc attggcttgg
     3781 atctcactga gcgcgaactg ctggatgagg ccggccctgg cgacatccgg cgattgcgcg
     3841 cgccggatca aggcgatcac ggcatcgagg ttgtccaggg cgattttcag gccttcgagg
     3901 atatgtgccc gctcttccgc cttgcgcagc tcgaacgccg ttcggcgcac gacaacttca
     3961 cgccggtgct caaggaagtg gtggaggatt tgcttcagat tgaggacttc cgggcgattg
     4021 ttgaccagcg ccaacataat cacgccgaac gtcgtctcga gctgcgtgtg tttatacaga
     4081 ttgttcagca cgacgagcgg gatttcgcct cgcttcaact cgatgacgat ccggacgcct
     4141 tcgcgggccg aggattcatc gcggagatca gagatgccct taagccggtc ttcctggatc
     4201 agttcggcga tcttctcgat caatttcgct ttgttgacct gatagggaac ttcggtgacg
     4261 atgagacgtt cgcggtctgt gcgttcgtcc gtttccacag ctaccttggc acggagcttg
     4321 aggaggccgc ggcccgtttc gtaggcatct ttgatgccgg tcatgccgta gataaagccg
     4381 gctgtcggga agtctggtcc gggaatcttt ttcatcaact gcgcgatcgt gacctctgga
     4441 ttctccaaaa gcagcagcag tccctcgacg acttcgccga gattgtgcgt gggaatgttc
     4501 gtggcataac cgaccgcgat tcctcccgct ccgttgataa ggagattcgg gacttttgtc
     4561 ggcagcacca acggttcttg ccgcgactcg tcatagttgg gaccgaaatc gactgtttcc
     4621 ttgtcgatgt ccgccaacag ctcttctgcc agcttcgtca tgcgcgcttc ggtgtagcgc
     4681 atggccgcgg ctgaatcgcc gtccatcgag ccgtaattgc cctgcccgtc caccagcgga
     4741 tagcgcatgt tgaattcctg cgccatacgc accagggtgt catagatcgc gctatcgcca
     4801 tggggatggt agttacccat gatctcgccg acgatcttgg ccgacttgcg gtaggcgcga
     4861 ttgtggacga ggcccatctc gttcatgccg aaaagaatcc ggcgatggac cggcttgagg
     4921 ccgtcgcgca catctggaag ggctcgtcct acgatcacgc tcatggcgta atcgagatac
     4981 gaggacctca tctcgtcttc gatggcgatg tggcctaatc gttcatcagg gggcatgtgt
     5041 tttccttagc tctcagcgat tagcattcag ccgtctgctc cgaacaagct gatagctgtc
     5101 agctgaaagc ttccttgcta gacatctaag tttctaactt cgagcgcatg cgcctgtatg
     5161 aaattccggc gcggttcgac ttcgtcgccc atcagaatcg taaaaatctc gtccacgcca
     5221 gtcacgtctt ccagctccac cttcaacagc gtgcgcattt ccggattcat cgtggtttcc
     5281 cagagctgct ccggattcat ctctcccaat cctttgtagc gctggatacc gaggccctgc
     5341 ttgccgaggt cgaggatcgt tttaaccata tcggcggtcg agtggagctg ctgctcttgc
     5401 cccttcgcct tcaacttgta gggcaggcgg cccagcccga tcgacgaggg agcgagtttt
     5461 tgcaactctc tgaattcagc ggagccgacc agctcgtgcg tgacgtccaa ttgatgcgta
     5521 atgcctccgg catgcatgcg gcaggacact ttgttcgact ggtgttcttc gtcgggaaaa
     5581 atctcgatcg tcggctcgag cttcggaaac accaacccga gcgccttctt cgcgttcgcc
     5641 accgttttct tcagcgcggc caaatccttg agcatgtcgc gatccagccc cggctcatcc
     5701 acgaagaccc tgagcatcgc ggcctcgtgc gatttcttgt cgagcctagt gagcagcgtc
     5761 tcgaacgaga tcattttctt caagatcggg aggagccggc gccccgtcac atagtcctgg
     5821 gccccctcca catacagctc cacttcttcg accgcgagat cggcgagatg ctcattcaac
     5881 gccccctcgt ccttgagata ccgctccgac ttgccctttt tcactttgaa gaggggaggc
     5941 tgggcgatat agatgtagcc ccgttcgagt agttccggca tctgccggaa gaagaacgtg
     6001 agcagcagcg tgcggatgtg gctgccgtcg acgtcagcgt cagtcataag aataatcttg
     6061 tggtagcggg ccttcgcgat gtcgaacgta tccttgtcgg ccttatcgct ctcctcgcgt
     6121 ttgcggccga tcccggtccc aagcgccatg atgagtgtcc ggatttcgtc gctcgtcagc
     6181 atcttgtcga agcgggcctt ttcgacgttg aggatcttcc ccttgagcgg cagaatggcc
     6241 tggaacttgc ggtcgcgccc ctgcttcgcc gacccgccgg ctgaatcgcc ctcgacgatg
     6301 tagagctcgc tgagggcggg atctttttcg gaacagtcgg ccaattttcc ggggagcga
//
LOCUS       DATIFR010000160         1298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_295494, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000160 DATIFR010000000
VERSION     DATIFR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295494"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VLD60_09515"
     CDS             <1..161
                     /locus_tag="VLD60_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB45238.1"
                     /translation="VLPKRWIVERTIGWLGRCRRLAKDWENLNHNALAFLRLASIRLM
                     LRKLCNPS"
     gene            complement(156..967)
                     /locus_tag="VLD60_09520"
     CDS             complement(join(156..583,583..967))
                     /locus_tag="VLD60_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612206.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSB45239.1"
                     /translation="MWTTENRARYDRSRLRYPSDLTDEEWALIAPRLPPARRGGNKRT
                     VNLREVVNGLMYILSTGCQWRAIPKDLPPRSTLYDYFDLWSWDGTLDRIHHALYVECR
                     ARAEREASPTTAIIDSQSVKSAEKGGPCIDPHGYDAGKKIKGKKRHILVDTQGLLMHA
                     IVHAADIQDRDGGVLLMATLFGIYPFLLKLYADGGYQGAGFQEALRTVLNRVNLEIVK
                     RSDQAQRFVVLPKRWIVERTFAWLGRCRRLAKDWECLNRKALAFLRVRPETL"
BASE COUNT          263 a          381 c          369 g          285 t
ORIGIN      
        1 tggttctgcc caagcgctgg atcgtcgaac gcaccatcgg ttggctcggt cgatgtcgtc
       61 gtctcgccaa ggactgggaa aacctcaacc acaacgcgct cgccttcctc cgcctcgcct
      121 ccatccgttt gatgctcaga aagctttgta atccctcata gagtctccgg acggactctg
      181 aggaaggcga gtgctttgcg attgaggcat tcccaatcct tggcgagcct tcggcatcgt
      241 ccgagccagg cgaaggtccg ctcgacgatc cagcgtttgg gcaagacgac gaagcgctgc
      301 gcttgatcgg agcgcttgac gatctcgagg ttaacgcggt tcagaacggt gcgcagcgcc
      361 tcttgaaagc cggcgccctg atatccgcca tcggcataga gcttcagaag gaagggatag
      421 atgccgaaca gcgtggccat cagaagcacg ccgccatcgc gatcctggat gtcggcggca
      481 tgcacgatgg cgtgcatcaa gaggccctgc gtgtcgacga gaatatgccg cttcttgccc
      541 ttgatcttct tgcccgcatc gtagccatgc gggtcgatgc agggccccct ttttcagcgc
      601 ttttcacgct ctggctgtca atgatggccg tggtcggaga cgcctcgcgc tcagctctcg
      661 cgcggcattc gacataaagg gcgtgatgaa tgcgatcaag cgtgccgtcc cagctccaca
      721 gatcgaagta atcgtacaag gtcgagcggg gcggcaggtc tttcgggatc gcccgccatt
      781 ggcagccggt gctcaggatg tacataagac cgttgaccac ttcgcgcagg ttcaccgtcc
      841 gcttgttgcc gccgcgcctg gccggcggaa ggcgcggcgc aatcagagcc cattcttcgt
      901 cggtcaggtc gctcggatag cgcaggcggc tgcggtcata gcgtgcgcgg ttttcggtcg
      961 tccacatcgg cggcggctcc ccatggaatc aggccgcctc tcttgaatca caaatgattc
     1021 agccgattca agatgttccc ggacagtcac taaggctttt atggaaccgc gtcatttcca
     1081 ctatgttcaa tacaatctac aacgtgttcc cagaggccga catacatgat gttatatttt
     1141 ccgttctgtc agggcgatat agttgcgagc ttcctgatga tgacatttac gggcttggct
     1201 cccccgaaaa gactatacaa ctgcgtctga tatactgtca gtctgcaagg caggctaatg
     1261 aaggaaagcg actatactat cacctcgccg aaacggaa
//
LOCUS       DATIFR010000161         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1829937, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000161 DATIFR010000000
VERSION     DATIFR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829937"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VLD60_09525"
     CDS             complement(<1..231)
                     /locus_tag="VLD60_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSB45240.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKVVAGVTPGKA
                     GQDVEGIPVFNTVRDAVKKAQCDTSLIFVPPPF"
     gene            complement(390..1568)
                     /gene="sucC"
                     /locus_tag="VLD60_09530"
     CDS             complement(390..1568)
                     /gene="sucC"
                     /locus_tag="VLD60_09530"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSB45241.1"
                     /translation="MNVHEFQAKSLFAQFGVPVPRGREVKTPQEAEAWAAELNSPVFV
                     VKAQIHAGGRGKAGGVKITKNRAEVPGLAKELIGKTLITHQTGPKGRVVHRLLIEEGA
                     NIAKELYLSILVDRDSGWPVFIASTEGGMEIEEVAAHTPEKIIKELIDPAVGFQGYNG
                     RNIAFALGINKIEPAAVNPFIQMLGNLYRLFMEKHAALVEINPLIITKEKTVVALDGK
                     VSFDDNGIFKHPDVQQMRDLNEEEPLEIEASENNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDPNVKGIFINIFGGIVRCDRIAHG
                     VIEAAKEVKITVPLVVRLQGTNAEEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            complement(1657..>2690)
                     /locus_tag="VLD60_09535"
     CDS             complement(1657..>2690)
                     /locus_tag="VLD60_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSB45242.1"
                     /translation="ADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLKDEVLNQKIQL
                     LEFAAAVELLSDDDGNCTGAIFKDLDNHRYVVVAAKSVILATGGIGRLHIQGFPTSNH
                     YGATGDGLCLSYRMGAKLAHIDTFQYHPSGAVYPEQLIGALVTEGIRSEGGHLVNAKG
                     ERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHGPGTLEKHFPA
                     MMLQFERFGIDISKDPVLIYPTLHYQNGGVKIDTNSETNVKNLFVAGEASGGLHGRNR
                     LMGNSLLDLMVFGKRSGLTAASRTGSMKQGKLTLKHLQRFRADAKKHGNVSGVISPMI
                     LPAYTRKVS"
BASE COUNT          529 a          836 c          701 g          624 t
ORIGIN      
        1 gaaaggcggc ggcacaaaaa tcaacgaggt gtcgcactgc gctttcttga ccgcatcgcg
       61 aacggtattg aagaccggaa tcccttcgac atcctgccca gccttgcccg gcgtcacgcc
      121 ggccacgacc ttcgtgccgt aggccttaca ctgggtcgca tggaatgagc cttccttgcc
      181 cgtaattccc tgcaccacca cccgcgtatt cttattgacg agaatgctca cgattccctc
      241 ttttctttct tatttcacct tcccatgacc gggaagcagt ctttcggagg cgcagggtta
      301 agcccccact gcgcgcgtcc aacgaggccc ttctgaaggc gcgcgttgcg cgagcacagg
      361 ggcttatcgc tgtgccttca tttgtcgtct cacgccgctt ttccggtcag cttcacaatc
      421 ttttgcgcgg cttcccacaa atcgttggcc acttccagct tgaggccgga ttccgccaac
      481 agcttacggc cttcttcggc attcgtgccc tgtaaccgga ccacaagtgg cacggtaatc
      541 ttcacttctt tggccgcttc gatcacgcca tgggcgatac ggtcgcaacg gacgatccca
      601 ccaaagatat tgatgaagat acctttcacg ttgggatcct taagcagaat gcgaaatcct
      661 gccgccacgg tttccttcgt cgcgccaccg cccacgtcca ggaaatttgc cggctcgctg
      721 cccgccagct tgatgacgtc catcgtcgcc atcgcgaggc cggcgccgtt caccatgcaa
      781 ccgatgttgc cgtccagctt gacgtagttg agattgttct cactcgcctc gatctccaac
      841 ggctcttctt cattgagatc gcgcatctgc tgcacatctg gatgcttgaa gatcccgttg
      901 tcgtcgaagg ataccttgcc gtccaacgcg accaccgtct tttccttcgt aatgatcaac
      961 gggttgattt cgacaagcgc ggcatgtttc tccatgaaca atcggtagag attgccgagc
     1021 atctggatga agggattgac cgcagccggt tcgatcttgt tgatgcccaa ggcaaaggcg
     1081 atattgcgtc cgttatagcc ctggaatccg acggctggat cgatcagctc cttgatgatc
     1141 ttttccggag tatgcgcagc gacctcttcg atctccatcc cgccttcggt gctcgcaatg
     1201 aagacgggcc agccggaatc gcgatccacg agaatactga gatagagttc cttcgcaatg
     1261 ttggcgcctt cttcgatcaa gagccgatgc acgactcgac ccttcggacc ggtctggtgc
     1321 gtgatgaggg tcttaccgat gagttccttg gcgagcccgg gcacctcggc cctgttcttc
     1381 gtgattttta cgccgccggc cttgccgcga ccgccggcat ggatctgcgc cttgacgacg
     1441 aataccgggc tgttcagctc tgctgcccag gcttccgctt cctgtggggt cttcacttcg
     1501 cgcccgcgcg gaaccggcac gccgaattgc gcgaacaacg acttggcctg aaactcatgc
     1561 acgttcatat tcgctcctta cagtcgcttc aatactgtgt gctgagcggc gagtgctgag
     1621 taatccaaat tccttctcag cactcagtcc tcagcactaa ctcactttcc tggtataggc
     1681 aggcaggatc atgggagaaa tcacgccgct gacattccca tgtttcttgg catcggcccg
     1741 gaaccgttgg agatgcttca acgtcaactt cccctgcttc atcgatccgg tgcgcgaggc
     1801 tgcggtcaat cccgaccgct tcccgaaaac catcaaatcg agcagcgaat tccccatcaa
     1861 ccgattgcgc ccgtgcaatc cgccggacgc ttcgccggcc acgaagagat tcttcacgtt
     1921 ggtttcggag ttcgtatcga tcttcacccc gccgttctgg tagtgcagtg tcggatagat
     1981 caggaccgga tccttgctga tgtcgatccc gaaccgctcg aactgcagca tcatggcggg
     2041 gaaatgcttt tcgagcgtgc ccggtccgtg ctcggcatcc agcaacgggg tgtctaacca
     2101 gacaccgaca cgacccgaca tcgtgcgaat accgcggcct tcttcgcatt cacgaataat
     2161 cgaggacgac acgacatcgc gcgtatcgag ttcgttcaca aagcgttcgc ccttggcatt
     2221 caccagatgg ccaccctctg aacgaatccc ttccgtcacg agcgcgccga tcaactgctc
     2281 cggatacacg gcacctgacg ggtggtattg aaaggtatcg atgtgggcca acttggcgcc
     2341 catccgatag gaaaggcaga gaccgtcgcc ggtggcccca tagtgattgc tcgtcggaaa
     2401 gccctgaatg tgcagacggc cgattccgcc ggtcgccaga atgaccgact tggctgcaac
     2461 gaccacatac cgatgattgt cgaggtcttt gaagatcgcg ccagtacagt tgccgtcatc
     2521 gtcgctgagc aattcgaccg ccgcggcaaa ttcgagtaat tggattttct ggttgagaac
     2581 ctcatcttta aggacccgca tgatctcgag gccggtgtaa tcggaacagg tcagaagacg
     2641 cggcttggaa ctgccgccgc ctttcttgac atggaggttc ccgtcggcct
//
LOCUS       DATIFR010000162        22433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_872450, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000162 DATIFR010000000
VERSION     DATIFR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872450"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VLD60_09540"
     CDS             <1..1049
                     /locus_tag="VLD60_09540"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HSB45243.1"
                     /translation="LLPDGGFELNTRIFDFHLARHRFFSPEFLRLLGANRQPEEEITQ
                     RHKDMAASAQLVLEETLLHLARYVKQLTGLDRLCLAGGVAYNCVANSRLWREAGFQEV
                     YVPPAAGDSGAALGAALWLAQRRGNLTERIVMRSASWGPDYSEQACLAALDQAGLQIE
                     RLPDTPLCEKVAAELSSGRLVFWFQGRMEWGPRALGNRSLLADPRREDMRALINAKVK
                     LREPFRPFAPSVLEERASEYFDLPGPSPFMLFTAPVQESAKGVIPAVVHVDGSARVQT
                     VDADSNPRYRLLLETFGRLTGVPVLLNTSFNVNEPIVCTPEDAVRCFLRTDVEWLVMG
                     NLLARRPAVVRRDT"
     gene            1046..2617
                     /locus_tag="VLD60_09545"
     CDS             1046..2617
                     /locus_tag="VLD60_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45244.1"
                     /translation="MRHKQSEGERSSGVKALSPSSPPAEVRFPAFDALLFLIGLNFAL
                     QPLTEPDFGWHLRAGLDFFKQGWTLPDVDPYSHTMADWAWVEHAWLTDVIIAWIYSLF
                     GGLGVILFFGAITMSAWLLASRLAPCGDLFRWLACVFSLWVALPYLGARTQLVTFLGL
                     SIVLVFLKRWQAGDRWPRWCIPPLFLLWANLHGGFTAGLLLLGLTIAVTAVVRLMASR
                     RILFTAGIDEILWSWQDLKKLVIVTVLSTLVTFMNPYGWRLHAEIIDSLSNQFMLASL
                     QEWQSFSTQGIAGRSYVLYLSGLGLGTALWYRRIEPVRWVIGACFLALSFRHMRNIPF
                     FLLFSLPLCAELLAIGFDRLEHWARPWIGAVRSWGMIGTIAGGLMLVWLGPEHLHHVV
                     HSGVRPAEYFRETSYPIEAVEWVQTHRDQLGQRLYNDYAYGGFLLWWLPEEKIFIDGR
                     MPAWHRGERRIFQDYAALTVADPPDLTILKKYFVDWAIVQKNSPLDQRIAQEAAWRRV
                     YEDEKVSLYRLLPER"
     gene            complement(2646..3461)
                     /locus_tag="VLD60_09550"
     CDS             complement(2646..3461)
                     /locus_tag="VLD60_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HSB45245.1"
                     /translation="MLRWQRTEAVQISVVIPAHNEASRILPYLLQITDYFRQQGRSHE
                     ILVVDDGSTDTTASVVEDFAGSVPTVHLIRMTSCRGKGAAVRCGMQEAGGDLQLFADA
                     DGATPICELARLEEALAEGADVAIGSRALASQLPDFSVRARLYRSVLGTLFNAVVQQC
                     GIRGISDTQCGFKLFRRVVAEELFAYSSIDGYGFDLELLYIARQRGYRITEVPVNWSD
                     QPGSKVRVLRDGLAMLGELALIRRNDRKGRYSLPSFSPDFLPVRAARFELPRN"
     gene            3772..4563
                     /locus_tag="VLD60_09555"
     CDS             3772..4563
                     /locus_tag="VLD60_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45246.1"
                     /translation="MRELLNAACLVLSAAVLYACASAFATGAPSRIDNYVSPDHRGKE
                     ESLAKLRQRPLQAGLVLVSDTAEPGAAAPNLPDEALVRLGESLKQEIGRVLPVTIREI
                     IQTDHIRPQPSGDWAQFTELGRQHGLDYLVVVVLSSMEQEYPVTLFLGWTTHAQPGFR
                     RDNWSLLEFALLDLKGNKTLMEAEGRGWATLDRPSAPGINQWYPAVYLRPQENRRIWP
                     PTYEGAPNTLKVVSFEQAVKRLTLRLQNSWLGQLEADEAARRTTS"
     gene            4734..6251
                     /locus_tag="VLD60_09560"
     CDS             4734..6251
                     /locus_tag="VLD60_09560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSB45247.1"
                     /translation="MAKWSMDGRALGLASCFCLFAIACTLNPIPGEPTAQAAGVPPAM
                     AQGFSDIVKKVTPAVVNIAVTGGGEGGRGRRPLPPGPFGGPPGGGGEEPPGGELPTPP
                     PMPPGPHGRPDQSAGSGVILDPNGYIVTNNHVVEGATQITVTLSDRREFSAKIIGTDP
                     KTDLAVIKIEAKDLPSVRWAEYEKLQVGDLVLAIGSPFGLSSTVTLGIISALGRGNVG
                     IADYEDFIQTDAAINPGNSGGALVNMNGDLIGINTAIFSRTGGSEGIGFAIPSSIALD
                     IVESLQRTGKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSHAAGVKR
                     GDVVIAFNGKEVQSVSQLRNLVARTVVGKDAQVKILRDGKEQTLMVKVAERPSDEMLA
                     KKEAAPPKEQGETVKPPDNVLASLRVQALDNATMSQLNIPAKTSGVVVTSVEPGGPAE
                     AAGIQRGDVIQEVNHEVVKSIDDYQKAAHKIKKEELTVLLLSRQGNNLFVAVNPK"
     gene            6312..7241
                     /gene="tatC"
                     /locus_tag="VLD60_09565"
     CDS             6312..7241
                     /gene="tatC"
                     /locus_tag="VLD60_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSB45248.1"
                     /translation="MLNDFNRWLQDSVFKPLEDKKMPVMEHLVELQVRLTRAVIVTAI
                     IFVGTFFYADTLVKWLRIPLQNMFVPGALSWVPTDLPAVPFVFLAPAEALWQNVKVAG
                     LFAIVIAMPYILLEVWQFVVPGLHAQERRFVGPFVLLSTLAFYAGVGFSFFFVLPFAL
                     NFLVSYGVNAGFIPQLSIAQYVGFALWFLMVFGLIFEVPLAITLMAKLGWVDAPFLKR
                     YRKWALLGAFIVAAILTPTPDPFNQCLMALPMYVFYEVGIISAGFFNKKKPPVPETAV
                     APVAAGGVKAMSSAMPGAADSDYVGVPTGSRRR"
     gene            7287..8240
                     /locus_tag="VLD60_09570"
     CDS             7287..8240
                     /locus_tag="VLD60_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSB45249.1"
                     /translation="MARELNIIQTPTSGGSDTCTYMWACAICDENESCQKDKEGHSRW
                     LVAKRMERIEYKVLVMSNKGGVGKSTCTTNIAVSLALKGWHVGICDMDIHGPNIPKMV
                     GAEGQKLKISTSGGIIPFQAYNLKIASMSFLLQNPDDPIIWRDAYKYEFINQLLGGVE
                     WQDLNFLLIDLPPGTGNESVTTIDLLGNVSGAVIITTPQEVALLDSRKSVTFCKDSEV
                     PIIGIVENMSGLDCPHCHHHIEVFRKGGGEASAQDMGVPFLGRIPLDPEVVTQSDAGE
                     PFAMFNSDTATAECYHDIANKVEAFCKKSGSLVNIAPRHAH"
     gene            8254..9552
                     /gene="glp"
                     /locus_tag="VLD60_09575"
     CDS             8254..9552
                     /gene="glp"
                     /locus_tag="VLD60_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSB45250.1"
                     /translation="MKAKDALSGLTLLEEAQRIVLDATLILGLEKISILDALGRVLGE
                     DIIVERDNPPWDNSAMDGFAVRWEDIKQEHAIQKPVTLTVIEDVPAGKMPRKTVGPGQ
                     AIRIMTGAPIPKGADTVLKVEDTEHTPDTVRVFKPEAQGSNIRPQGEDVKKGERIIAK
                     GTTIRPGEAGMLAILAKSFVFAYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQDAGGIPFLLGIARDNPAALKEKISHGLTADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKMSGHRSYG
                     RPVVQAIFQEKFSKRTDRRHFLRGVLTREDGTFKVRTTGDQGSGILTSMVKANCLIDV
                     PVEVERLNPGDTVSVQLLSGEAWASHAEHTNAGPQRLSCC"
     gene            9574..10095
                     /gene="mobB"
                     /locus_tag="VLD60_09580"
     CDS             9574..10095
                     /gene="mobB"
                     /locus_tag="VLD60_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HSB45251.1"
                     /translation="MAVPIVCFVGRSNSGKTTFIERVIPELVRAGYKVATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVILSKGSMAMFADVSDQMSVEDVRDRFLDGSYDLIIAE
                     GWKHEGFPKIVIIREQVGEIPISTEGLLAVVSDKEVNLNVPVFALDDVPAVASLIMKH
                     FPRRAQSEHELEA"
     gene            10079..10786
                     /locus_tag="VLD60_09585"
     CDS             10079..10786
                     /locus_tag="VLD60_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HSB45252.1"
                     /translation="MNWKPSATIILALVAGAVALGGAAIPLTNHPKFCASCHNIAPSY
                     ESWVKSSHKEVTCVSCHVRPGVEGWIHDKAWAGTKDVAIYLFGTPTDSHNLKSSVDSD
                     VCLGCHRNILRVSEVAPRDLPAPVKDVGLIMSHRKHMDAFRVRGQGEGCTTCHSGVVH
                     DQPIKGYPIVVPRGHVSADSKPWHPDYPEGSYLRTRALNDCFRCHDGKAEHQGRMLDR
                     KCETCHLPDKIKDFLSF"
     gene            10805..11536
                     /locus_tag="VLD60_09590"
     CDS             10805..11536
                     /locus_tag="VLD60_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB45253.1"
                     /translation="MSAPVLQVTSLTKRFGEFVAVNDVSFAIKPGEILGLLGPNGAGK
                     TTTIHMLLGLITPTAGSISMFGLDLATQREQILQAVNFSSTYISLPLALTVEENLWVV
                     ARLYGMSDIARRIDEVVKKLEMEEFRHKITRKLSSGQMTRLTLAKAFLTEPRILFLDE
                     PTASLDPDIAHKIRALLKEERRSSGLSILYTSHNMREMEEMSDRIIFLQRGRIVAEGT
                     AQEIVERFGQADLEEVFLKLAREPH"
     gene            11551..11958
                     /locus_tag="VLD60_09595"
     CDS             11551..11958
                     /locus_tag="VLD60_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45254.1"
                     /translation="MQNGLLDFGIGAVLGGLAMSACWGLFWLAIGTVGLTRRTCSWRV
                     VLNGLTVLLIPLLLGCLLIWGRGVFLYGSAFGAGLFVVPLVLMGFALRQAPDGQRVGA
                     HMLGGVRQLIDELLGKQHACGGCSHEHEHGGDP"
     gene            11955..12746
                     /locus_tag="VLD60_09600"
     CDS             11955..12746
                     /locus_tag="VLD60_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB45255.1"
                     /translation="MKLHRVAAIIARHLYLYRRSLPRIMEIFYWPFLDLVIWGFITLY
                     LAKYQPQVPGFVTFFLGALILWDMLFRSQQGITISFLEELWARNLMNLFASPLTPGEF
                     LTATIAMSIFKVTCVSFVMGICAWVFYGYNVLVIGLWLLPFIVNLVIAGWIIGVLTTS
                     LIMRFGQEAEILAWSMVFLFQPISCVFYPLEVLPVWLQGVAWINPAAHVFEGMRAVLS
                     GSGSPLLHLAWAGGLNGILLIAVVALFYKTFAYCKEQGSLVRVGE"
     gene            12970..13308
                     /locus_tag="VLD60_09605"
     CDS             12970..13308
                     /locus_tag="VLD60_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB45256.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFGAHGAVASARIITDKFTGQSR
                     GFGFVEMSSDSEAQAAITALNGSEMGGRTLTVNEARPQEPRSGGGGGGRGGFGGGGGR
                     SGGGGKRDRW"
     gene            13815..14411
                     /locus_tag="VLD60_09610"
     CDS             13815..14411
                     /locus_tag="VLD60_09610"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HSB45257.1"
                     /translation="MLVIEDFWSAEERQYFREAMTKASWKSVQDLPNVREDFPNSGNW
                     ARAEIAPPQSQRFFPRLQLRCVQEHIESFPNIVGRHLGFNYYSYAVGDCLLTHDDTDQ
                     GYAMKGKRAPLRRIAVVSYFHEEWHSDWGGELIIYKKRDGHTSEKPDLEVTHCIEPRS
                     GSLVMFTVPRFHRVCRVDETAGQHRRMSIAGWFMTEHS"
     gene            complement(14531..15046)
                     /locus_tag="VLD60_09615"
     CDS             complement(14531..15046)
                     /locus_tag="VLD60_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45258.1"
                     /translation="MSAPNGALPSGARPTVLAVDDDPDALKLCCLFLEKAGFSVLPAT
                     GSSEALKICKNHPGPIHLLVTDIVMPPPDFSFASGDNEFPHVHGHELALRALKMRKDL
                     RVILTSANIDKDLQGYGIRPDCLPFLSKPLDAQALATTVAQTLQSPPPTPESLTNDRS
                     SGAKGADEWFD"
     gene            complement(15239..15940)
                     /locus_tag="VLD60_09620"
     CDS             complement(15239..15940)
                     /locus_tag="VLD60_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45259.1"
                     /translation="MSYRSGSNLRNTCLTIAAMLLTLTGCQTYNTHPVTQTDGSYTRL
                     PNASTKVVVWGTRPEAVQSLTTWLSKRGLILVDDVKMSQIANDIRLNQPGTSVSNADV
                     FKLGKIVGAKQVIFIDADVTTWRASEIHSFFGQDPAIYTASVFIRALDAETGEIQWNG
                     KALSMDKFTNLKEGIHQLTCHALATAWGLREPGSTTTPTICPPGQNVMVFTPPTPAPS
                     GTATTADSGGSRRTP"
     gene            complement(16111..17562)
                     /locus_tag="VLD60_09625"
     CDS             complement(16111..17562)
                     /locus_tag="VLD60_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HSB45260.1"
                     /translation="MALLATDIDSVLSEIAPILLNGWIQKIQQPSDRMIIFEIRVPGK
                     THRLLISCEPMAARLHLCRHPLPSPQSPPPFCQFLRAHLQGARIAGIQQLPNDRIVIL
                     QLATKDGSRQVVCELTGKSANVLVLDVNQCVMRDLNRHPQIIGRPYQAPVIRSAGAAD
                     GKTSRFAVPAGSSDFPISAAIEAHYYDQEAGLAQARARDARLRALRKTIKKEQRLIEA
                     WQDDLMKAAKYRGYARYGELIKANLISIRKGMDRIAILDYYDDAMPEVTIPLDPTKSP
                     QGNMDDYFRKYRKYLAAERELKPRIEQAEHELGEMREELAAIEQGIWTPPLSAAPDSR
                     IPTRKSSRTQQKSIDRRHGPFRRFVSSDGLPIFVGRNARENDELTFGLAKSDDLWLHA
                     RGAPGSHVVVRLEKGKDPPPETLRDAATLALLYSDLKKSGKGEVIYTRRKWVKKAKGQ
                     APGAVIVTQEKSLLVNFEKARLAALKSRSEELL"
     gene            17878..18105
                     /locus_tag="VLD60_09630"
     CDS             17878..18105
                     /locus_tag="VLD60_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45261.1"
                     /translation="MRCTKCEGLMVVDDLIDMRESYHPMWMRGWRCVACGNVVDPLIL
                     RHRMIQEAGALGFLKIEPPQPMYFRLAKSSA"
     gene            18528..19031
                     /locus_tag="VLD60_09635"
     CDS             18528..19031
                     /locus_tag="VLD60_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45262.1"
                     /translation="MSQTKTAVDDSDMLLLTSVALLPVERDDDMRSRLAQIFQQQTTL
                     RVESTSEPEGGIADSLTDEDRSAIAKDLTKELVVDAVLFGRVSEISAHPSDWGWKQEE
                     SRRLYLHLVDHEGRLLWKDELPYTVVIGAKPPIEHAVQTSLTHHVMDHVRDLRLDDLG
                     YFPRKPS"
     gene            19101..20663
                     /locus_tag="VLD60_09640"
     CDS             19101..20663
                     /locus_tag="VLD60_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HSB45263.1"
                     /translation="MSLLDAATLTSLQSEFSQSMRTLLMDLCRDIDEHYSELAERLEF
                     PVAYFRILARSFDLESYAHWKVVGWIEALNDLVYFLDLLQQIRKEPDQRAFAKQLFAE
                     CEEKYFENSYLDDLFPQGRAQAKGLAARLQWLCMKLAREVTQESLFFDPRWAMQWSGR
                     QGLGKWEVPGDIDPHFERAERAFTLSIGTIGDELVAPKSVVQGVGRTAPRIIFEITKQ
                     GIALKKGRRFPVCSVQGANVEWHWPRQAAVEAARTSQGPLTIGRTLRYGKDRQPQKVI
                     STSPKQVARIARAWHTIREAWPKGYEVLALLTSRVIPLKAKGVVSFSYRHRPGLSFIN
                     CFDRDNLDLIDDLIHENSHHHLNLLLRKHVMYHGDHNQQIFYSPWRRSLRPLRGILHA
                     TFTFTMGAMLFERLSSWAESTRGMKGWKEAGLTQRDLQRARFRCLEEIESVRYSIQDL
                     NYAGRHLKWLTGSGGRLVRQLEEEITEAEQRIARHRIAVLKSKFGPALRRHVKELQQA
                     RMTYGPVRLSKA"
     gene            complement(20693..21754)
                     /locus_tag="VLD60_09645"
     CDS             complement(20693..21754)
                     /locus_tag="VLD60_09645"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSB45264.1"
                     /translation="MGRKGLGVRREAQKIALVAFMAAFALSLSLQPAHAFKITEPVEG
                     ATLKPGQTITAHVDLGNDIGIVKVRYYWYGEQDDTLVGQEDATATGSIVAPVALIGLS
                     NHDPAFGGPLLIPKDGIGPMRLLAVAEISRGRLGTRTVFDEILVTVEPAAALNTIDFE
                     TDKPLQLGRTGQSSAFGHVDSLGKIFELPVVGEFADGVTRRISSPASGSAYQSSNPKV
                     IRVLPQGLLQIVGNGKTVLTVTNRGKQASLDIGVTVNDEPNEPPVADAGVNRTVKSGT
                     KVKLSGLKSRDAEGEALFYSWSQVRGSKVSLLDVNGPEATFQAPQVSEPRQYRFKLRV
                     TDKKGADSVPAFVDITVEP"
     gene            complement(21757..>22433)
                     /locus_tag="VLD60_09650"
     CDS             complement(21757..>22433)
                     /locus_tag="VLD60_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB45265.1"
                     /translation="SFTLAQTGMVRRWLIKKGPHWRKKLVVNGIGAVTTGIATVIIAS
                     TKFMHGAWIVFLLVALLILMFRGIRSHYKAVTEQVSLSRDARPPRPRRNIIVVPIGGV
                     NRSVVRAVDYARSRPGEIRAVLVDVDKEETARVEIQWAQWGCGVPLIVLPSPYRSVLG
                     SLLDHIEELRQKDPDCWITVVIPEILPARWWQNILHNQRALMLKAALLFKDRVVLTDV
                     PFHLTR"
BASE COUNT         4676 a         5969 c         6692 g         5096 t
ORIGIN      
        1 gtcttctccc cgatggcggg ttcgaactga atacgaggat cttcgatttt cacttggccc
       61 gccatcgttt tttttcaccc gaattcttgc gactcttagg tgccaatcgg cagccagagg
      121 aagagatcac gcagcgccat aaagacatgg cagcaagcgc ccagctggtc ctggaagaga
      181 cgctcttgca cctcgcccgg tacgtgaaac agttgacggg gttggatcgg ctctgcctgg
      241 ccggcggagt cgcctataac tgtgtagcaa acagtcgact ctggcgtgag gcaggatttc
      301 aggaagtata tgtgcctcct gcagcaggcg attcaggggc tgcactcggt gcggcgctat
      361 ggctggcaca gaggcggggg aacctcactg aacgaatagt catgcgatct gcctcctggg
      421 gaccggacta ttctgagcag gcctgtctgg ccgcgcttga ccaagccggt ttgcagattg
      481 aacgtcttcc cgatactcct ctgtgtgaga aagttgccgc ggagttatcg agtggccgtt
      541 tggtgttctg gtttcaaggg cgtatggaat ggggaccccg agccttggga aatcgaagcc
      601 tcttggccga tcctcgccga gaagacatgc gggccctgat caatgccaag gtcaagcttc
      661 gtgaaccgtt tcgtcccttc gcgccatccg tactcgaaga acgggcctcc gagtactttg
      721 atttgcccgg tccctcgccg ttcatgctct ttaccgctcc ggtacaagag tcggccaagg
      781 gagtcatccc ggccgtggtg catgtcgacg gttcggccag ggtacaaacc gtcgatgccg
      841 attcgaatcc acgctaccgc cttctccttg aaacatttgg tcgcctgacc ggtgtgccgg
      901 tgctgctcaa tacctcgttt aacgtgaacg aacctatcgt ctgtacgccg gaagacgcgg
      961 tgaggtgctt cctgcgcaca gatgtggagt ggctggtgat gggcaatctt ttggcgagac
     1021 gaccggcggt tgtgaggcgg gacacgtgag gcataagcag tctgaaggag agcggtcttc
     1081 aggggtcaag gccctgtccc cttcatcccc tcccgctgaa gtgagatttc ccgcgtttga
     1141 tgctctgctg ttcctcatcg gtttgaactt tgcccttcag ccgctgaccg aaccggattt
     1201 cggctggcac cttcgagcag gattggattt cttcaagcag ggctggaccc tgcccgacgt
     1261 ggatccctat tcccatacga tggcggattg ggcgtgggtc gaacatgcat ggctgaccga
     1321 cgtgatcatt gcctggatct attcgctctt cggaggactc ggcgtgattc tcttcttcgg
     1381 cgcgatcacc atgagcgcct ggctgcttgc cagccgcctc gcaccctgcg gagacctgtt
     1441 tcgatggctc gcctgcgtct tctccctctg ggttgcgctg ccttatcttg gcgccaggac
     1501 tcagctcgtc acgttcttgg ggttgagcat agtgcttgtc ttcttaaaaa ggtggcaggc
     1561 cggcgatagg tggcctcgat ggtgtattcc tcccttattc ctactgtggg ccaatctgca
     1621 cggaggcttc acggcggggc tgctgttgtt ggggctcacg atagccgtga ctgcagttgt
     1681 cagattgatg gcgtctcgtc gaatactctt caccgccggc attgatgaaa tcttgtggtc
     1741 atggcaggac ctcaagaaac ttgtcatcgt gacggtcctt tctacactcg tcaccttcat
     1801 gaatccctac ggttggcgat tgcatgccga aatcatcgac tcactgtcga accaattcat
     1861 gctcgcttcg ttgcaggagt ggcaatcttt ttcgacgcag ggaatagccg gacgcagtta
     1921 cgtgctctac ctgtcgggac ttggcctggg gacggccctg tggtatcggc ggatcgagcc
     1981 ggttcgatgg gtcatcgggg cgtgcttcct ggctctgtct tttcggcata tgcggaacat
     2041 tccgtttttc ctcctcttca gcctacccct ctgtgcggag cttttggcga ttggattcga
     2101 tcggctagaa cattgggcgc ggccgtggat cggggcagtt cggagctggg gtatgatcgg
     2161 aacgatcgca ggtgggctga tgctggtgtg gctcggcccc gagcatctcc accacgttgt
     2221 tcactccggc gtaagaccag ccgagtattt cagagaaacg tcttatccga ttgaggcagt
     2281 ggaatgggtg cagacgcatc gggatcagtt gggccaacga ctctacaatg attatgcgta
     2341 tggcggtttc ttgctgtggt ggctgccgga ggaaaaaatt ttcatcgacg gacgaatgcc
     2401 tgcctggcac aggggagaac gccgcatatt ccaggattat gcggcgttga ccgtggccga
     2461 tcctcctgat ctcactatct tgaagaagta cttcgtcgat tgggccatag tgcagaaaaa
     2521 cagtccgctg gaccagagaa ttgcccagga agcggcctgg agaagagtct atgaagacga
     2581 gaaggtttcg ctctaccggc tcttacctga acggtgacga cgaaaataat ggaatgggaa
     2641 tccgcctaat ttctgggaag ttcaaaccga gcggccctga ccggtaggaa atcgggcgag
     2701 aaggagggaa ggctgtaacg tcccttacga tcatttcttc tgatcaaagc taactcgccg
     2761 agcatggcaa gtccatccct caggactcga acctttgagc ctggctgatc cgaccagttc
     2821 accggcacct ctgtaattcg atagcctcgt tgtcgggcta tatagagcaa ctcaagatcg
     2881 aatccatacc catcgattga agaataggcg aagagttctt ctgccacaac cctgcggaat
     2941 aatttaaatc cgcactgggt gtctgatatc cctctaatcc cgcattgctg aacgactgca
     3001 ttgaagagcg tgccaagcac gcttctatac aggcgcgcgc gaacggaaaa atccggcaac
     3061 tgtgaggcca gagcccgcga tccaattgcg acatcggctc cttctgccag ggcctcctcc
     3121 agccgagcga gttcgcaaat gggagtggcg ccatctgcgt cagcaaacag ttggaggtct
     3181 ccccctgcct cctgcattcc acaccggacg gcagcacctt tgcctcggca tgacgtcatt
     3241 cgaattagat gtacggttgg aacagatccg gcaaaatctt cgacgaccga agcggtcgta
     3301 tcggtactgc catcgtcgac gacgagaatt tcgtgtgagc gaccttgctg gcggaagtaa
     3361 tcagtaatct ggaggagata gggaagaatg cgcgaggcct cattatgggc cggaatgacc
     3421 acggagatct ggacggcttc cgtcctttgc catcggagca tggatcacca gattctgtga
     3481 gaatctcgcg gcaaaacgag ccagacttcc gcgcgtaccg tagccgagat ccagaggaaa
     3541 aacaaggggg gccatgggct tgacagatcc ggtctagggg agcgaacgta taaataaaaa
     3601 agtggagcga ctcgggcagc gttcctaaaa agggacgtga gagatggatg gctggagtgt
     3661 gaggactgga gaggtaaccg caagacggac tggaaaatga tcaaatgatc agcagttcat
     3721 ccggtttata agtgaggatg agtctgcaca gccgggagta ggaggtgggt gatgcgtgag
     3781 ctactcaatg ccgcgtgcct tgtgctgagc gctgccgtgt tgtatgcctg tgcctctgcc
     3841 tttgcaaccg gcgctccgtc tcgaatagat aattatgtat cgcccgatca tagaggcaaa
     3901 gaggagtcgc tggcgaagct caggcagcga ccccttcagg ctggcctggt actggtgtcg
     3961 gatacagcgg aaccaggtgc ggctgctccg aatcttcccg atgaagcgtt ggtgcgactg
     4021 ggggagagcc tgaagcagga gatcggtcgt gtcctccctg tcaccattcg ggagatcatt
     4081 caaaccgatc atattcgtcc ccaaccaagc ggagactggg cgcagtttac agagttgggc
     4141 cggcaacacg ggctcgacta tttggtcgtc gttgtcctgt ccagcatgga acaggaatat
     4201 cccgtgacgc tgttcctggg ctggacgacc cacgcgcagc cgggcttccg ccgcgataac
     4261 tggtcactct tggaatttgc actcctcgat ttgaaaggca ataagacact gatggaagcc
     4321 gaaggccgag gctgggcgac gttggatcgt ccgagtgccc cagggattaa tcaatggtat
     4381 ccagcggtct atttacgccc tcaagagaac cggagaatct ggcctccgac ttacgagggc
     4441 gcaccgaaca ccctgaaagt tgtgtcgttc gagcaagcgg tgaagcggtt gacgttgagg
     4501 ctccagaata gttggttggg ccagctggaa gccgacgagg ccgcacgcag aacaacgtca
     4561 taatttgcgc ggtcggcagc cgattattcg agccacccac gagtcccctt cgggcatttc
     4621 cgcatcagtc cgaaggggac tatttgtctt tgaaggcttc ggaatggatg gtgtacaatg
     4681 caccgttcat ccgtctaacg tcggtttcta taaccttctg gaggaacccg gttatggcta
     4741 agtggagtat ggatggtcgt gccctcggcc ttgccagttg tttctgcctc tttgcgatcg
     4801 catgtaccct caatccgatt ccaggcgagc ctacggcgca ggcagcggga gttcctccgg
     4861 cgatggctca agggttctcg gacattgtga aaaaagtgac gccggctgtg gtcaatattg
     4921 ccgtgaccgg cggcggcgaa ggagggcgag gcaggcggcc gcttcctccc ggtccgtttg
     4981 gaggacctcc gggcggtggt ggagaagagc ctcccggtgg ggaacttcca acgcctcctc
     5041 cgatgccacc aggtccccat ggacggccag accaaagtgc tggttccggt gtcatcctgg
     5101 atccaaacgg gtatatcgtc acgaataatc acgtggtcga aggggccacg cagataaccg
     5161 tgacgctcag cgatcggcgc gagttttccg cgaagatcat cggaacggat ccgaaaacag
     5221 acttggctgt catcaagatc gaggcgaagg atctgccttc tgtcaggtgg gccgaatatg
     5281 aaaaactcca ggtcggagat ctcgtgttgg ccatcggcag cccctttggt ctgagctcta
     5341 cggtcacgct cggtatcatc agcgcgctcg gacgagggaa tgttggcatc gccgattatg
     5401 aggatttcat tcagacggat gccgccatca atcccggtaa ttccggcggg gccctcgtga
     5461 acatgaacgg cgatctcatt ggcatcaaca ccgccatctt ctcacggacc ggtggctctg
     5521 aaggcatcgg ttttgccatt cccagcagca ttgcgttgga catcgtcgag agtttgcaaa
     5581 ggaccggtaa ggtcgtgcgc ggctggatgg gcgtggcaat tcaagagatt acgccggcgc
     5641 tggcgaagtc gttcaaactg ccggaacaac ggaagggtgt cttgatcagc gacgtcaacg
     5701 agaatggccc gtctcacgcg gccggggtca agcggggaga cgtggtgatt gccttcaacg
     5761 gcaaggaagt gcagagcgtc agccagcttc gcaacctcgt agcccgcacg gtcgtaggga
     5821 aggatgcgca agtcaagatc ctgcgagacg gcaaggagca gacgctcatg gtcaaagtgg
     5881 cggaacggcc gtcggacgag atgctcgcga agaaagaagc cgcgcctccc aaggagcagg
     5941 gcgagacggt caagccgccc gacaatgtgc tggcatcgct cagagtgcag gcgttggaca
     6001 atgcgaccat gagtcaattg aatatcccgg cgaaaacgag cggcgtagta gtgacctcgg
     6061 tggaaccggg tggtccggcg gaggcggcgg gaattcaacg tggagacgtg attcaagagg
     6121 tgaatcacga agtggtcaag agcattgatg attaccagaa ggcagcgcac aagattaaga
     6181 aggaagaact gaccgtgctg ctcttgagtc gccagggaaa caacttgttt gtggcggtga
     6241 atccgaaata ggtggaaatt tctgggtgcc agccgccacg gctggcaccc aggactcatg
     6301 ctggggaccg tgtgctgaac gacttcaatc gatggttgca agactctgtc ttcaagccac
     6361 tggaagacaa gaaaatgccg gtcatggagc acctcgtgga gctccaggtc cggctcacgc
     6421 gggctgtgat cgtgacggcg atcatattcg tcggcacgtt tttttatgcc gacacgctag
     6481 tcaaatggct gcgcattccc ctccaaaaca tgtttgtgcc cggtgctctt tcctgggtgc
     6541 cgaccgatct gccggccgta ccgttcgtgt ttttagctcc tgctgaagcg ctctggcaaa
     6601 acgtcaaggt ggctggcctc tttgcgatcg tcatcgccat gccatacata ctcttggaag
     6661 tgtggcagtt tgtcgtgccg ggtctgcatg ctcaggagcg ccggttcgtc ggcccgtttg
     6721 ttctgttgag cacgctggct ttttacgcag gcgtggggtt ttcatttttc tttgtcctgc
     6781 cctttgcctt gaattttctc gtctcgtacg gcgtcaacgc cggatttatt ccccagctct
     6841 cgatcgcaca gtatgtaggc tttgccctct ggttcctcat ggtattcggg ttgattttcg
     6901 aggttccact ggccattacg ttaatggcca aattgggttg ggtcgacgcg ccgtttttga
     6961 agcgttaccg caaatgggcc ctcttaggcg cgttcattgt tgctgcgatt ctgaccccca
     7021 cgccagatcc cttcaatcaa tgcttgatgg ccttgcctat gtatgtgttt tacgaagtcg
     7081 ggatcatcag tgccggattc tttaacaaga agaaaccgcc ggttccggag actgccgtgg
     7141 cgccggttgc cgcggggggg gtcaaagcca tgtcgtccgc aatgccaggc gctgcagaca
     7201 gcgactatgt cggggtacca actgggagcc ggcgccgctg agaggatcat ccttttaagc
     7261 agataagtag aaagggcacg agaaccatgg ctcgcgaact gaacatcatt caaactccga
     7321 cttcgggcgg gagcgatacc tgcacctata tgtgggcctg tgcaatctgt gatgagaacg
     7381 agtcctgtca gaaagacaag gaaggccata gccgctggct tgtcgccaag cggatggagc
     7441 ggatcgagta caaagtactc gtcatgagca ataagggcgg cgtcgggaaa agcacctgca
     7501 cgaccaatat tgcggtgagc ctggcgctca aaggctggca cgttgggatc tgcgacatgg
     7561 atatccacgg gccgaacata ccgaagatgg tcggtgccga agggcagaag ctcaagatca
     7621 gtacgtcggg cggcatcatc ccgtttcaag cctacaacct gaagatcgcc tccatgtcgt
     7681 tcttgctgca gaatccggac gatccgatca tctggcgcga cgcgtacaag tacgaattca
     7741 tcaatcaact cttgggtggc gtggaatggc aggacctgaa ttttctgttg atcgatttgc
     7801 ctcctggcac cggcaacgag tccgtgacga ccatcgattt gctcggcaat gtcagcggcg
     7861 cggtgatcat tacaaccccc caggaagtcg ccctcttgga ctcgcgcaaa tctgtcacgt
     7921 tctgcaagga cagcgaagtg ccgatcatcg ggatcgtcga aaatatgagt ggtttggatt
     7981 gcccccactg tcatcaccat attgaagtct tccgcaaggg cgggggagaa gcgtcggcgc
     8041 aggatatggg cgtgcccttc ctggggcgga ttcctctcga ccccgaggtg gtcacccagt
     8101 cggatgccgg tgaaccgttc gccatgttca attctgacac ggccacggcc gagtgctatc
     8161 acgacatcgc gaataaggtc gaagcgtttt gtaagaagag cggctcgctg gttaacatcg
     8221 cgccgcggca tgctcattga cgagggagag accgtgaagg ctaaggacgc actatcgggg
     8281 ttgaccctgc tcgaagaggc gcagcggatt gttctcgatg ccactctcat cctggggttg
     8341 gagaagatat cgattcttga cgccctgggt cgcgtgctgg gcgaggacat tatcgtcgaa
     8401 cgagacaatc cgccctggga caacagcgcc atggatggat ttgccgttcg ctgggaagac
     8461 atcaagcaag agcatgcgat ccagaaacct gtgacattga ccgtgattga ggatgtcccg
     8521 gccggcaaga tgccgaggaa gacggtcggt cccggacaag cgatccgtat catgaccggc
     8581 gctccaattc ctaagggagc cgatacggtg ctcaaagttg aagatacgga gcacacgccc
     8641 gataccgtcc gggtgttcaa accggaagcg cagggttcaa acatccgccc gcagggtgag
     8701 gacgtgaaga agggcgagcg gatcatcgcg aaaggcacga ccattcgccc cggcgaagct
     8761 gggatgctgg cgattctcgc caagtcgttc gtgtttgcct accaacgacc gcgagtggcg
     8821 atcctctcga ccggcgatga attggccgac cttgacgagc ggtttagtga ggagaaaatc
     8881 atcaactcaa acagctatgg tattgcggcc gcggtacagg atgcgggagg tattcctttt
     8941 ctcctcggca tcgccaggga taaccctgcc gcgctcaagg aaaagatttc gcatgggttg
     9001 acggcggata tcctcgtcct ttccggcggg gtgtcgatgg gtgactatga tttcaccaag
     9061 gccgtcttcc gtgacctggg tgccgaaatg aatttctgga aattggcgat cagaccgggg
     9121 cagccgttgg cgttcggaaa gattcagggc aaacttgcat tcggattgcc ggggaatcct
     9181 gtttcttcca tggtgacctt cgagcaattg gtccggccgg cgatgctcaa aatgagcggg
     9241 catcgcagct atgggcggcc cgttgtccaa gccatatttc aagagaagtt ttccaagcgc
     9301 accgaccgtc ggcacttcct ccgcggtgtg ctgacgagag aagatggcac gttcaaggtc
     9361 aggacgaccg gcgatcaagg gtccggaatt cttacgtcga tggtcaaggc caactgcctg
     9421 atcgatgtgc cggtcgaagt cgagcgattg aacccgggcg atacggtgtc cgtgcagctc
     9481 ctgagcggtg aagcttgggc cagccacgcc gaacatacca acgcggggcc gcagcggctc
     9541 tcgtgttgct gaggcacgaa aggattttgc accatggccg tgcctattgt ctgtttcgtc
     9601 gggcggtcca atagcggtaa aaccaccttc atcgaacgcg tcattcctga actcgtccgg
     9661 gccggataca aggtggccac ggtcaagcac gccggccatg ggttcgatct cgacacggag
     9721 ggcaaggaca gctggcgcca caaacaggcc ggcgccagca gtgtggtgat cctgtcgaag
     9781 ggcagcatgg ccatgtttgc cgacgtctcg gatcagatgt cggtggagga cgtccgggat
     9841 cggttcctgg acggttccta cgatctcatc attgccgaag ggtggaagca cgaagggttc
     9901 cccaagatcg tgatcatccg cgagcaagtc ggggaaattc cgatttcaac agaaggtttg
     9961 ctggcggtcg tatcggataa agaggtgaat ctgaatgtgc cggtctttgc cctcgacgac
    10021 gtgccggcgg tggcctcgct catcatgaaa cattttccga gacgtgctca atcggagcat
    10081 gaactggaag cctagcgcta cgatcatact tgcacttgtg gccggagccg ttgcgctcgg
    10141 tggcgcggcc attcccctca cgaatcatcc caagttctgc gcgagttgcc acaatatcgc
    10201 tccctcgtat gagagttggg tcaaatcgtc tcataaagag gtaacgtgcg tctcctgcca
    10261 tgtcaggccc ggtgtcgaag gatggatcca tgacaaggcc tgggccggaa ctaaggatgt
    10321 cgccatctat ctgttcggga ccccgactga ttcgcacaat ttgaaatcca gcgtcgactc
    10381 cgatgtctgc ctcggttgtc accggaatat cttgcgcgtc tccgaggttg cgcctcgtga
    10441 tctgccggcg ccagtcaaag atgtcggcct tatcatgagc catcggaaac acatggacgc
    10501 ctttagggtt cggggccagg gagaaggatg cacgacgtgt cattccggtg tggtgcatga
    10561 ccagccgatt aagggatatc cgatcgtcgt tcctcgtggg cacgtttcgg ccgacagtaa
    10621 accgtggcat cccgattacc cggaggggtc gtaccttcgg acgagagcgc tcaacgactg
    10681 tttccgctgc catgacggaa aagccgagca tcaaggcagg atgctcgacc gaaagtgcga
    10741 gacctgtcat ctccctgaca agatcaaaga ctttctgtct ttttaacgag ggccttgctg
    10801 gattatgtct gccccagttc tccaagttac gagtctgacg aagcgcttcg gggagtttgt
    10861 cgcggtgaac gacgtgtcgt tcgcgatcaa gccgggtgaa attctcggat tgctcgggcc
    10921 gaatggagcg ggcaagacga cgacgataca tatgctgctg gggttgatca cgccgactgc
    10981 cgggtcgatc agcatgttcg gactggatct ggccacgcag cgcgagcaaa tcttacaggc
    11041 ggtgaacttt tcatcgactt atatctcgtt gccgctggcc ctcaccgtcg aggaaaatct
    11101 gtgggtggtg gcgcggttgt acggaatgtc ggatattgcc cggcgtatcg acgaggtcgt
    11161 caagaagctg gaaatggaag agttccggca taaaattacc agaaagctct cctctggaca
    11221 gatgaccagg ttgacactgg ccaaagcgtt cctcactgag ccaaggattc tttttctcga
    11281 cgagccgacg gcgagtctcg atccggatat cgcgcataag attcgtgcgc tgctgaaaga
    11341 agaacggcgc tcgtccgggc tcagcatcct ctacacgtcc cataacatgc gcgagatgga
    11401 ggaaatgtcg gatcgcatta tttttcttca gcggggcagg atcgtagccg agggaacggc
    11461 ccaggagatc gttgaacgat tcggtcaagc cgatttggaa gaagtcttct tgaagcttgc
    11521 gcgggagccg cattgaggga gtcgttcttt atgcagaatg ggttgcttga tttcggaata
    11581 ggggcggtgc tcggcggctt ggcgatgagc gcctgttggg gactgttctg gctcgcaatc
    11641 gggaccgtcg ggctcactcg tcgcacgtgc agttggcggg tcgtgctcaa cggcttgacg
    11701 gtcttgctca ttccgctgct gctgggatgc ctgctcatct gggggcgagg ggtcttcctg
    11761 tacggctctg cattcggagc gggcttgttc gttgtgcctc ttgtcctgat gggatttgcc
    11821 ttgcgccagg ctccggatgg ccagcgagtt ggcgcgcaca tgcttggagg ggtgcgacag
    11881 ctcatcgatg agttgctagg gaagcagcat gcatgcggcg gttgcagcca tgagcatgaa
    11941 cacggaggcg acccatgaaa ctccatcgtg ttgccgccat catcgcccga catctttatt
    12001 tgtaccgccg gagtttgccc aggatcatgg agatcttcta ttggccgttc ttagatctcg
    12061 tgatttgggg attcattacc ctctacctcg caaagtatca gcctcaagtg ccagggttcg
    12121 tgaccttttt tcttggagcc ttgatcctct gggacatgct gtttcgctcg cagcagggca
    12181 tcaccatttc atttctcgaa gaactttggg ccaggaactt gatgaacctg tttgccagcc
    12241 ccctgacgcc cggggaattt ctcactgcca cgatcgcgat gagcattttc aaagtcacgt
    12301 gtgtttcgtt tgtgatgggg atctgtgcct gggtgttcta cggctataac gtcttggtca
    12361 taggcctttg gctgcttccg tttattgtga atctcgttat cgcaggatgg atcatcgggg
    12421 tcttgacgac ttcactgatc atgcgattcg ggcaagaagc ggaaattttg gcctggagta
    12481 tggtctttct cttccagccg atctcctgcg tgttttaccc gctggaggtg ttgcccgtct
    12541 ggcttcaagg ggtcgcctgg atcaatcctg ccgcccacgt ctttgaaggc atgcgcgcgg
    12601 ttctcagcgg ttcaggctcg ccgctcctgc atctcgcctg ggccggcgga ctcaacggga
    12661 tactcctgat cgccgtagtc gccttgtttt acaagacctt cgcctattgc aaggagcagg
    12721 gctcgctggt gagggtcggg gagtaaggtt gtaatccacc agaccctatg ttagggttgg
    12781 gggacttcat cgtacgggcc tcgcggtgaa tctggttctc aaagagcagg cccgcaacat
    12841 cgatgttgcc ttgtccggga atttcgtcgg aagtcagacc taagtcgccg catccttcgc
    12901 gtcttcggcc cgctccttcc ccgttcctcg agttcaagac aatgtcatca ttcaaaggag
    12961 gaaccccaca tgggttcgaa gatttacgtc ggtgggttgc cctattcggc gaccgagcag
    13021 cagttaagcg atctgtttgg cgctcacggc gcggttgcgt cggcgcggat tatcacggat
    13081 aagttcacgg gacaatcccg cggatttggt ttcgtggaaa tgtcctcgga ctccgaggcg
    13141 caggcagcga ttacggcgct caatggttcc gagatgggcg gccggacgtt gacggtcaat
    13201 gaagcgcgtc ctcaagagcc gcgttccggt ggcggaggcg gaggccgcgg tggattcggt
    13261 ggcggcggcg gtcgcagtgg tggcggagga aagcgcgatc gctggtaagc cgtactactc
    13321 gtgaagcaag accgggggct gtcatcatat cgatgacagc ccccgttgta tttcccctaa
    13381 gcaaaacatc gggctaagga aagcccgatt gtcctgtctt gtgatgtccc atgggaaagt
    13441 ggtaagacgg cgcaagaacg agaccgcgca gggcgggcta gcttatttaa tgccggtttt
    13501 ctctttcatt attcgtagcg tacccagatc ccttcgtgcc tgcccgtcag tacacagccg
    13561 gtggaatagc gctgtgtgct cccctgtatg gtcctcgtgc tggtttgcat aacttcaacg
    13621 tgtccgtaga agtgagtctc tcgatctgtc cctgagggat tgtgccggtc tgtgaatcct
    13681 tccaacaaat tggaggggtg tgctctggca attccattgg gctgtatgag gatttgatgc
    13741 ccgatagtcg gagtgcgtta ctgacagcga gccgatcagg gaacctggag tcccattcgt
    13801 ttcaggagaa ccctgtgctc gtcattgaag acttctggtc ggctgaagaa cggcagtatt
    13861 ttcgtgaagc aatgacgaag gcctcctgga agagtgtcca agatttgccg aacgtgcggg
    13921 aggattttcc aaactcaggg aactgggcta gggccgaaat cgcaccgcct cagagtcaac
    13981 ggttttttcc gcgactgcag ctccgctgtg ttcaggaaca cattgaatca tttcccaaca
    14041 tcgtaggccg gcatctgggc ttcaactact actcatatgc cgtcggagat tgtttgctga
    14101 cccacgacga cacagaccag gggtatgcca tgaaggggaa gcgtgccccg cttcgccgga
    14161 tcgcggtggt gagctatttt cacgaagagt ggcactcgga ttggggcggg gaactcatca
    14221 tctacaagaa acgggacggt catacgtctg aaaagccgga tctcgaagtg actcactgca
    14281 tcgagcctcg atctggctcc ctcgtcatgt tcaccgtgcc gcggttccat cgcgtctgtc
    14341 gagtcgacga gacggcaggc cagcatcggc ggatgtccat cgcaggctgg ttcatgacgg
    14401 agcattcgta gcggtctgga aatgagcgaa cgatgagggg gtgcttgtca gagacaggca
    14461 ccccctcatc gttcgacgca tgcgcaggca ctgctctgaa gacaggcaca aaatcgtcga
    14521 aacgcaggaa ctagtcaaac cactcgtccg ctcccttggc accgctgctg cgatcgttcg
    14581 taagcgattc gggggtaggc ggaggagatt ggagggtttg tgccactgtc gtggccagcg
    14641 cctgcgcgtc gagcggcttg gacaggaagg gtaggcaatc aggccgtatt ccatagccct
    14701 gcaggtcttt atcgatattc gctgatgtca ggatcacgcg caggtctttc cgcattttga
    14761 gagcgcgtag agcaagttca tggccatgga cgtgggggaa ttcattatcc cctgaagcaa
    14821 aggaaaaatc cggtgggggc atcacgatgt cggtcaccaa gagatgaatc gggcctggat
    14881 ggtttttgca gatcttgagc gcttcggaac tgccggttgc cgggagaacc gaaaaccccg
    14941 ccttctccag aaacaagcag caaagtttca aggcatccgg gtcatcgtca acggccaaga
    15001 cggtcggtcg cgcgccggat gggagggcac cattgggggc tgacatggga tacctcctgc
    15061 ttacgcggat cactgtactc aatgttcagg atagcccaga tattcggaat ccccaaacga
    15121 atggtgaaat ttgttaggga gaggaatcag ccagttggaa ggaagcaccg tgcccggtgc
    15181 gcttttgatt ttggatgttg aaacgggagg cgggtggctg ctagtttccc gcttctagct
    15241 agggggtgcg cctgctccct ccgctgtcag cagttgtggc agtgccgctt ggcgcggggg
    15301 taggtggggt gaataccatc acgttctgtc cggggggaca gatggtagga gtagtagtcg
    15361 atccaggttc acgtaacccc caggcagtgg caagagcatg acaggttaac tggtgtatgc
    15421 cttccttcaa gttcgtgaat ttgtccattg aaagcgcttt cccattccac tgaatttctc
    15481 cggtctccgc atccagtgca cggatgaaga cagaagccgt ataaatggcc ggatcttgtc
    15541 cgaagaagct atggatttcg ctcgcccgcc aagtcgtcac atccgcatcg atgaaaatga
    15601 cttgctttgc tccgacaatt ttgcccaatt tgaacacgtc ggcgtttgag accgaggtgc
    15661 ctggttggtt caaacgaatg tcgttcgcta tctgactcat tttgacgtcg tcgaccaaga
    15721 tgagtcctcg tttcgagagc caggttgtga gactttgaac ggcttccgga cgagttcccc
    15781 acacgactac tttagtggac gcgttgggaa gtctggtata ggacccgtca gtttgtgtca
    15841 cgggatgcgt gttgtaggtt tgacagccag ttaacgtcag cagcatggct gcaatggtta
    15901 ggcaggtgtt tcgtaggttg gaccctgatc tgtatgacat tggtgcctcc tctaaggtca
    15961 gcgtgggtca ctctacagag cggggctggc aagccacaat tccctaaagg tacaggaccc
    16021 ctgcctttcg aggcatacct cggggccaat ttgaacgaag ggcagcatgc cggtatgccg
    16081 ggagggtcca atgagaggaa gcctgatcgg ctagagaagc tcttcgctcc gcgacttgag
    16141 tgccgccagt ctcgcctttt cgaagttgac caggagagat ttctcctgcg tgacgatcac
    16201 ggcaccaggc gcttggccct tggctttctt gacccatttg cgcctggtat agatcacttc
    16261 accttttccg cttttcttga gatcgctgta gagcagagcc agcgtggcag cgtctctcaa
    16321 ggtttcgggt ggtggatcct tccccttctc cagccgtacg accacatgtg aacccggcgc
    16381 gccccgcgca tggagccaca gatcgtcgct tttggcgagg ccgaacgtca gttcgtcgtt
    16441 ctcccgcgcg ttgcgcccga caaagatcgg caatccgtcg gaagaaacga agcgcctaaa
    16501 aggcccatga cgtcggtcga tcgatttctg ttgtgtgcga gagctctttc gcgtgggaat
    16561 acgtgaatca ggggccgctg ataagggcgg ggtccatatg ccctgctcga tcgccgcgag
    16621 ttcttcacgc atctcgccca attcatgctc ggcctgttca atgcgcggtt tcagttcccg
    16681 ctctgcagcc aggtatttcc gatattttct gaagtagtcg tccatgttgc cttgaggcga
    16741 ttttgtaggg tcgagcggga tggtcacctc gggcatggca tcgtcgtaat agtccaggat
    16801 ggcaatgcgg tccatcccct tcctgattga aatcaaattg gccttgatca attctccata
    16861 gcgggcataa ccccggtact tggccgcctt cattaagtcg tcttgccagg cttcaatcag
    16921 gcgctgttct ttcttgatgg tcttccggag cgcgcgcagt cgtgcatctc tcgcacgcgc
    16981 ttgggccagt ccggcttcct ggtcataata gtgggcttcg atcgccgcgg agatcggaaa
    17041 gtcgctcgag ccggctggca cggcgaatcg ggaggtcttg ccatccgcag cgccggcgct
    17101 gcggatgacc ggcgcttggt acgggcggcc aataatctgg gggtgacggt tcagatctcg
    17161 catgacacat tggttcacat caagcaccaa gacatttgcg ctcttgcctg tgagttcgca
    17221 cacaacctgg cgtgatccat ctttggtggc gagttgaagg atgacaatcc ggtcgttcgg
    17281 taattgttga atcccggcta ttctggcccc ttgcagatgg gcgcgcagga attgacagaa
    17341 gggaggtgga ctttgcgggc tcggtagggg gtggcggcat aaatggagcc gggccgccat
    17401 tggttcacaa gagatgagga gtcgatgggt tttccccggc acacggattt cgaaaatgat
    17461 catccgatct gatggttgct ggattttttg aatccagccg ttcaagagaa ttggagcgat
    17521 ttcgctcaag acggagtcaa tgtcggttgc aaggagtgcc attggcaata ttggcgaccg
    17581 tgtgcgaaga aagcgtcctt ctgcctacta acgagcaacc gtatgcatga atctacgtgg
    17641 aaccactagt aggccctgcc attttcgtcc catggggaca atccggtcac attttcgtct
    17701 gtgagcctca cagctctggc tgccggcact ctacgattcg ccgagaaaac acgcaagcag
    17761 gcaggaatct aaggtggcat tcggcttgct ttacctagcg atgacacgag cctgcgagat
    17821 atcgagtgag ctggatcaat aggcggtggg caactcacgt gagcacagga gggtcccatg
    17881 cgttgcacga agtgtgaagg gctcatggta gtcgatgatt taatcgatat gagagaaagc
    17941 tatcacccca tgtggatgcg ggggtggcgt tgtgttgcct gcggcaatgt ggttgatccg
    18001 ctgattcttc gccaccgcat gattcaagag gccggtgcct tgggtttttt gaaaatcgaa
    18061 cctccccagc ctatgtactt ccggcttgct aagtcttcgg cctaagtgga gatacttgtg
    18121 tcgtcagtgc actgactcct tagtgcctgt tttaaggcgg gccgcaaacc gtccggtcta
    18181 gtacagagtg tccgcacttt ttcctcactg cacagtcact ccctgatcgc cacgcagaaa
    18241 agctcaataa cctcgacagg ttaaggaaat catgtgattg tttcagcagg gtatcgctct
    18301 tgctcaaaac cataaatagg tcggacaaaa aggaaagcag gggaatgagg aacatgaact
    18361 atgatccctc aattcttcgg tcggcgatga ggaccggggt cctcgttgct cttttcgcct
    18421 gcagcagtct attgcaaggt tgtctcgcag tggcttgggt tgccgtcgtt ggtgtggatt
    18481 ccctcaggtc gagcgatatc acgttctggc ctttcgaaca gagttgggtg tctcagacga
    18541 agacggcagt ggatgattcg gacatgctct tgctgacgag tgtggcgtta ttgcctgtcg
    18601 agagggatga cgatatgagg agtcggctgg cgcagatctt tcaacaacag accacgttgc
    18661 gtgttgagtc gacgtctgag cccgaaggcg gaatcgcaga ttcattaacc gatgaagacc
    18721 gctccgcaat cgccaaagat ctgacgaaag aactcgtcgt ggatgcggtc ctgttcggac
    18781 gcgtgtcgga gatctcagcg catcccagtg attggggatg gaagcaggaa gagtcccgac
    18841 ggctgtatct gcatctcgtc gatcatgagg gccgtttgtt gtggaaagat gaacttccct
    18901 acacagttgt cataggggcc aagcctccga tcgagcatgc cgtgcaaacc tcgctcactc
    18961 accatgtgat ggatcacgtc cgcgacctta ggctggacga cctcggatac tttcccagaa
    19021 agccttccta gctcgcggct tcttgcagcc tccgccgcgc tccggtagag tcccgttctc
    19081 gccgggagtt aaacgggacc atgtcattgc ttgatgctgc aacgcttacc agcctgcaat
    19141 cggaattcag ccaatccatg aggacgttgc tcatggatct gtgtcgggac atcgatgagc
    19201 attattccga actggcagag aggctcgagt ttccggtcgc ctacttccgc atcctcgcgc
    19261 gctccttcga tctcgagtcc tatgcacatt ggaaagtcgt ggggtggatt gaagcgctga
    19321 atgatcttgt ctacttccta gacctccttc agcagattcg gaaggagccg gaccaacgcg
    19381 catttgccaa acagctcttt gccgagtgcg aagagaagta tttcgagaat agttatctcg
    19441 atgacttgtt tccgcaaggc cgcgctcagg ccaagggact tgctgccaga ttgcagtggc
    19501 tttgtatgaa actcgcgcgt gaggtgactc aggagtcgtt gttcttcgat ccccgttggg
    19561 ccatgcaatg gagtgggcga caagggctcg gcaaatggga agtccccggg gatatcgatc
    19621 cgcattttga acgggcagaa cgagccttca ctctctcgat tgggacgata ggtgatgaac
    19681 tcgttgcgcc gaagtccgtc gttcaaggcg tcggccggac cgcgccgcgc atcatttttg
    19741 aaataacgaa gcaagggatc gcgttgaaga aaggcaggcg gtttcctgtc tgttcggttc
    19801 agggcgcaaa cgtcgaatgg cattggccga gacaagcagc ggttgaagct gcaagaactt
    19861 cacaaggacc gctgacgatc gggcggacgc tgcgctacgg taaagaccgg cagccgcaga
    19921 aggtgatttc aacgagtccg aagcaggtcg cgcgcatcgc gcgagcttgg catacaatcc
    19981 gggaggcctg gccgaaaggg tatgaggtcc tcgcgcttct cacttcccgc gtcatccccc
    20041 tcaaggcgaa aggcgtcgtg agtttcagct atcgccaccg gccagggctt tcgttcatca
    20101 actgttttga tcgggataat ctcgatctga tcgatgatct catccatgaa aacagtcacc
    20161 accatttaaa tctcttattg cgcaagcatg tcatgtacca cggcgaccac aaccagcaga
    20221 tcttctattc gccttggcgg aggagccttc gcccgctacg cggcattctc cacgcgacgt
    20281 tcacgttcac gatgggcgcg atgttgtttg agcggctctc gtcctgggcc gaatcgacga
    20341 gggggatgaa gggatggaag gaggcagggc tgacccagcg agaccttcag cgagcacggt
    20401 ttcggtgcct cgaagaaatc gaatcagtcc gctactcgat tcaagacctc aattacgcag
    20461 gtcggcatct caaatggctg accgggtcag gtggccggtt agtgagacaa ctcgaagaag
    20521 aaatcaccga agccgaacag cggatcgctc gtcaccggat tgcagtgctc aaatccaaat
    20581 tcggtcctgc cttgcgccgc catgtgaaag aactccaaca agcccgcatg acctacgggc
    20641 cggtacggct cagcaaggcg tagttctaga tatccagagg atcatataga cgctagggtt
    20701 cgacggtgat gtccacaaat gccggcactg aatccgcgcc ttttttatcc gtcaccctca
    20761 gcttgaatcg gtactgcctc ggctcggaga cttgaggggc ctggaaggtg gcttccggac
    20821 cgttgacatc cagtagcgac accttactgc cgcgcacctg gctccaggag tagaacagcg
    20881 cttcgccttc cgcgtcgcgg ctcttcaatc cgctcagctt gaccttcgtc cccgatttta
    20941 ctgtacggtt cactcccgca tccgcgaccg gtggttcgtt cggttcgtca ttgactgtca
    21001 cgccaatatc gagggaggct tgcttgccac ggttcgtcac cgtgagcacc gttttcccat
    21061 tgccgacaat ctgtaagagg ccctggggca acacccgaat gactttgggg tttgacgatt
    21121 gataggctga gccggaagcg ggggaactga tacgtcgcgt gacgccatct gcgaactcac
    21181 cgacgaccgg cagttcgaaa attttcccca acgagtctac atgaccgaat gccgaagatt
    21241 gtccggtgcg gccgagctgc aaaggcttgt ccgtttcgaa gtcgatcgta ttcagcgcag
    21301 cggcaggctc gacggtcacg agaatttcat cgaagacggt cctggtgcct aatcgcccgc
    21361 gggaaatttc tgcgacggct aacagccgca tgggtccgat accgtctttg ggaatcagta
    21421 gcggaccacc aaaggccgga tcatgattgg acaggccgat cagcgccacc ggcgcaacga
    21481 tcgaacctgt cgccgtggcg tcctcctgtc ccaccagggt gtcatcctgc tcgccgtacc
    21541 aataatagcg gaccttgacg atgccgatgt cgttccccag gtccacatga gctgtgatgg
    21601 tttggcctgg tttgagcgtg gctccctcaa ccggctccgt gattttaaac gcatgggccg
    21661 gctgcagcga gaggcttaga gcgaaagctg ccatgaatgc gaccaaagca atcttctgcg
    21721 cctcacgcct cacgcctaac cccttacgtc ccatgcttac ctcgtcaaat ggaagggcac
    21781 gtctgtcaga acgacgcgat ctttgaacag cagtgccgcc ttcaacatca aggcgcgctg
    21841 attgtgcaga atattctgcc accagcgagc aggcaaaatt tcagggatga caacggtgat
    21901 ccaacaatcg ggatcttttt gccggagttc ttcaatatgg tccagtaagg agccgaggac
    21961 cgagcgataa ggcgagggga gcacgatcaa cggtaccccg caaccccact gggcccattg
    22021 gatttcgacc cgcgccgttt cttctttgtc cacgtccacc agcacggcac ggatctctcc
    22081 aggacggctc ctggcgtagt cgacagcccg gacgacggac cggttgacgc ctccgatggg
    22141 cacgacaatg atatttctcc gaggacgcgg tgggcgagcg tcacgggata aggagacctg
    22201 ttcggtcaca gccttgtagt gcgatcgaat gcctcgaaac ataagaatca agagggcgac
    22261 cagcaggaag acgatccagg caccatgcat gaatttcgtg ctcgcgatga tgaccgttgc
    22321 gatgccggtt gtgaccgctc cgatgccgtt gacgacgagc ttcttgcgcc agtgtggacc
    22381 tttcttgatt aaccagcggc ggaccatgcc cgtttgagca agtgtgaatg aga
//
LOCUS       DATIFR010000163         8047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_106022, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000163 DATIFR010000000
VERSION     DATIFR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106022"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="rplF"
                     /locus_tag="VLD60_09655"
     CDS             complement(<1..207)
                     /gene="rplF"
                     /locus_tag="VLD60_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSB45266.1"
                     /translation="MSRIGKKPITVPAGVDVKVAGAVVSVKGPLGKLDWTLTPGIGVT
                     VDKGQVIVTRSSEDRNIRAMHGLVR"
     gene            complement(243..638)
                     /gene="rpsH"
                     /locus_tag="VLD60_09660"
     CDS             complement(243..638)
                     /gene="rpsH"
                     /locus_tag="VLD60_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSB45267.1"
                     /translation="MVTDPISDLLVRLRNGFRRQHDVVSIPASKLKREVLRVLQAEGF
                     IQGIENDTEDGHPVLKVQLRYVGEGQPMITGMQRVSKPGRRVYVGTKNIPRVRNGIGM
                     AILSTSKGIMTDQASRKAGLGGEVLCSIW"
     gene            complement(713..898)
                     /locus_tag="VLD60_09665"
     CDS             complement(713..898)
                     /locus_tag="VLD60_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HSB45268.1"
                     /translation="MSRLALRNKTAAKPKFPTRGYHRCGLCGRVHGYLRRFRMCRICF
                     RLLSLKGEIPGVRKSSW"
     gene            complement(920..1579)
                     /gene="rplE"
                     /locus_tag="VLD60_09670"
     CDS             complement(920..1579)
                     /gene="rplE"
                     /locus_tag="VLD60_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSB45269.1"
                     /translation="MADVKDAKKGKGGKPAGRPAKKDSASAPKVLDDGSEESKFQPRL
                     RDVYRDQVVPALMKEFGYKNVMQVPKLERIVLNVGMSEAIQNVKLLESAAGELGTITG
                     QKAVVTRAKKAIAGFKLRQGMPIGAKVTLRNRRMYEFFDRLVSLALPRIRDFRGVSPK
                     AFDGRGNYTLGLKEQLIFPEIQYDEVASIHGMDITIVTTARTNDEGKALLRQLGMPFR
                     T"
     gene            complement(1596..1925)
                     /gene="rplX"
                     /locus_tag="VLD60_09675"
     CDS             complement(1596..1925)
                     /gene="rplX"
                     /locus_tag="VLD60_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSB45270.1"
                     /translation="MDALQKIRIRKGDTVMVIAGRDRGKSGKVLSVDRTAGKIVVEKL
                     NMIKRHTKPNQKVKQGGILEKEAPLAISNVMFLCPVTQKPTRLGVRTQADGRRVRFSK
                     KSNELVE"
     gene            complement(1950..2318)
                     /gene="rplN"
                     /locus_tag="VLD60_09680"
     CDS             complement(1950..2318)
                     /gene="rplN"
                     /locus_tag="VLD60_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSB45271.1"
                     /translation="MIQNYTYMDVADNSGAKQAMCFHVLGGSKRRYASLGDIIVVAVK
                     EAIPQATVKKGDVSRAVIVRTTKEVRREDGSYIKFDRNACVLINKDGEPIGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            complement(2357..2623)
                     /gene="rpsQ"
                     /locus_tag="VLD60_09685"
     CDS             complement(2357..2623)
                     /gene="rpsQ"
                     /locus_tag="VLD60_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSB45272.1"
                     /translation="MAETEVKRREWVGRVVSNKMNKTVIVAVERSVVHPIYRKVLRRM
                     TKFKAHDEQNVCNVGDRVRMIETRPISKDKHWRVVEVLEKGRAE"
     gene            complement(2642..2851)
                     /gene="rpmC"
                     /locus_tag="VLD60_09690"
     CDS             complement(2642..2851)
                     /gene="rpmC"
                     /locus_tag="VLD60_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSB45273.1"
                     /translation="MELKELRQMTGAELSEKAKQLTQELFNLRFQLGTGRLENPMQIR
                     KTKRTIAQVKTILREVEQADQKKAS"
     gene            complement(2888..3301)
                     /gene="rplP"
                     /locus_tag="VLD60_09695"
     CDS             complement(2888..3301)
                     /gene="rplP"
                     /locus_tag="VLD60_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSB45274.1"
                     /translation="MLAPKKVKFRKMMKGRMRGKAYRGGHITLGEFGLKALEPGWVTS
                     RQIEAARIAITRYVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     LYEMDGVTPEIAREAFRLASHKLPIATKCVTRGEF"
     gene            complement(3316..3987)
                     /gene="rpsC"
                     /locus_tag="VLD60_09700"
     CDS             complement(3316..3987)
                     /gene="rpsC"
                     /locus_tag="VLD60_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSB45275.1"
                     /translation="MGQKTHPIGYRLGYNYTWSSRWYAGKDYAKLLHQDIKIRKVVKQ
                     KLYHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAELEKQYGGQVYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMVAGRLGGAE
                     IARTEWYREGRVPLHTLRAEIDYGFAEAHTTMGQIGVKTWIYKGELLPAQPLKPEAAF
                     ERRLG"
     gene            complement(4047..4400)
                     /gene="rplV"
                     /locus_tag="VLD60_09705"
     CDS             complement(4047..4400)
                     /gene="rplV"
                     /locus_tag="VLD60_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSB45276.1"
                     /translation="MAEARAVLRFVRVAPRKARPVVDMIRGQQVPMALAMLKHLPRHA
                     ARVVEKILRSAVANAEQKELGDSESMRVSKAFVNCGPIYKRFRARSMGRANAIQKRTS
                     HITVVVEAPGVTGKS"
     gene            complement(4452..4739)
                     /gene="rpsS"
                     /locus_tag="VLD60_09710"
     CDS             complement(4452..4739)
                     /gene="rpsS"
                     /locus_tag="VLD60_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSB45277.1"
                     /translation="MPRSISKGPFVDDHLLKKVEQMNQTKDRKIIKTWSRRSTVVPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            complement(4732..5574)
                     /gene="rplB"
                     /locus_tag="VLD60_09715"
     CDS             complement(4732..5574)
                     /gene="rplB"
                     /locus_tag="VLD60_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSB45278.1"
                     /translation="MGLKAYKPTTSGRRGMTIVTTDELTKKKPEKSLTSFHLRTAGRN
                     NDGRTTVRFRGAGHRRLYRKIDFRREKVGISAKVAAIEYDPNRSARIALLNYKDGEKR
                     YILAPVGLQVGDVVQSGIDAEVRTGNSLPLANMPLGTTIHNIELKPGKGGQLIRSAGG
                     FAQVMGRDGDYVQVRLKSGEMRRILASCMATVGQVGNLDHENITVGKAGRTRWLGRRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGLPTKGYKTRNNKATDKFIIARRKSG
                     VRNA"
     gene            complement(5644..5937)
                     /locus_tag="VLD60_09720"
     CDS             complement(5644..5937)
                     /locus_tag="VLD60_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSB45279.1"
                     /translation="MKLDLHSVLIQPLLTEKVTAMREANNTVSFLVHPDANRVQIKQA
                     VEALLKVKVDRVNVLNVQGKIKRLGRFSGKRSDWKKAFVKLKAGEKLELYESA"
     gene            complement(5934..6557)
                     /gene="rplD"
                     /locus_tag="VLD60_09725"
     CDS             complement(5934..6557)
                     /gene="rplD"
                     /locus_tag="VLD60_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSB45280.1"
                     /translation="MPTVDVVDLNRRKVGSAELPAEVFGCAPHTALVHEAVIMQRACE
                     RQGTASTLRRGEVAGSGKKPWKQKHTGRARAGSVRSPVWRHGGSVFGPKPRSYAFSMP
                     KKKYRAALQSALSAKVAGGHLVVVSSLSLEQPKTKLLAKALGQFGAGGQVLIVAGEGQ
                     TGLTQAARNLAKVSVVTPDRLNVYDVVRAGMIVIPERDLARVKEVWA"
     gene            complement(6562..7182)
                     /gene="rplC"
                     /locus_tag="VLD60_09730"
     CDS             complement(6562..7182)
                     /gene="rplC"
                     /locus_tag="VLD60_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSB45281.1"
                     /translation="MTNGLLGKKLGMTQMLDGARLAPVTVIEAGPCRVVMVKTKERDG
                     YEAVQLSFGEVKDRKLSKAELGHLKKHEAPASRILKEFKKDGDVAVGQVIKADLFKKG
                     DWVDVIGVSKGKGFQGVVKRHHYAGGPESHGSMFHRAPGSIGASSFPSRVWKGKTLPG
                     HMGAERVTTQRLKVIESRPDENLLFVSGAVPGAANGLLVVRKSKKG"
     gene            complement(7211..7522)
                     /gene="rpsJ"
                     /locus_tag="VLD60_09735"
     CDS             complement(7211..7522)
                     /gene="rpsJ"
                     /locus_tag="VLD60_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSB45282.1"
                     /translation="MKVDQRIRIRLRGFDYRVLDQSVAEIVETVRRSGAKVVGPIPLP
                     TRIEKFTVQAATHADKKAREQFEIRTHKRLMDIMEPTPETMDSLMKLNLAAGVDVEIK
                     L"
     gene            complement(7561..>8047)
                     /locus_tag="VLD60_09740"
     CDS             complement(7561..>8047)
                     /locus_tag="VLD60_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HSB45283.1"
                     /translation="RGIVKVGDEIEIVGLKPTQTTVVTGVEMFRKVLDEGQAGDNIGV
                     LLRGTKKEDVERGMVLAKPKSITPHTKFKAEIYVLTKEEGGRHTPFFNGYRPQFYFRT
                     TDVTGIVSLNPGVEMVMPGDNVSVTAELISPIAMDQGLRFAVREGGKTVGSGVVTEIL
                     A"
BASE COUNT         1531 a         2700 c         1899 g         1917 t
ORIGIN      
        1 gcgaaccagc ccatgcatag cccgaatgtt gcggtcctcg ctggatctcg tcacgatgac
       61 ctgcccttta tcaaccgtca ctccgatgcc cggcgtcaac gtccagtcga gtttccccag
      121 cgggcccttc acggacacaa ctgctcccgc cactttgaca tcgactccgg ctggtactgt
      181 gatcggtttc tttcctatgc gtgacatcgc tttctcatcc ctctcgctaa ttcagaacgc
      241 cgctaccaaa tggagcagag aacctctccg ccaagccctg ctttgcggga agcctggtcc
      301 gtcataatgc ctttggaggt ggacaaaatg gccatgccga tgccgttcct cacccgcgga
      361 atattcttcg tgcctacgta tacacgccgt ccgggcttgc tcacacgttg catccccgtg
      421 atcatgggtt gcccttcccc cacataccgc aactggacct tcagcacagg atgcccgtct
      481 tccgtgtcat tttcgatgcc ctggatgaat ccttccgcct ggagcacccg caacacttca
      541 cgcttcaact ttgaagccgg aatactgaca acgtcatgct ggcgccggaa accgttccgc
      601 aatcgaacta acaaatcgct tatcggatct gtgaccatac caaccctttc accaatatcc
      661 tattcgacaa catcatgccg gcctttcccc aatacgaccg gccataacac cattaccagc
      721 tggactttct gactcccgga atttcgccct tcaagctcag cagtcgaaag cagattctgc
      781 acatgcgaaa ccgacgcagg tacccatgca cgcgaccgca caatccgcac cggtgatagc
      841 ctcgggtcgg gaacttcggc ttcgccgccg tcttatttct cagtgctagt ctcgacactg
      901 ctgactcctt cgttctgtgt taggtccgga acggcattcc caactgtctg agcaacgcct
      961 tcccttcatc gttcgttcgg gccgtcgtca caatcgtaat atccattccg tgaatcgacg
     1021 ccacttcgtc gtattggatc tccggaaaaa tcagctgctc cttgaggccc aacgtgtagt
     1081 ttccgcggcc atcaaacgcc ttgggagaaa cgcctcgaaa atcgcgaatg cgcggcaagg
     1141 cgagactcac aagccgatcg aagaattcgt acatccggcg attgcgcagc gtgaccttcg
     1201 cgccaatcgg catcccctgc cgcaacttga atcccgcgat ggcttttttc gccctggtga
     1261 cgaccgcctt ctgccctgtg atcgtaccca actcaccggc agcgctctcc aacagcttga
     1321 cgttctgaat cgcctcgctc atacccacgt tcaggacgat gcgttccagc tttggcacct
     1381 gcatcacatt cttgtaccca aactctttca tgagagcggg cacaacctga tcgcgataca
     1441 catcgcgcag acgcggctgg aacttcgact cttcgcttcc atcgtccaac accttgggtg
     1501 cggatgcgct gtccttcttg gctggccgcc ctgccggctt gccgcctttt cctttttttg
     1561 cgtcctttac gtccgccatg cgaccgtcct cactcctact caacgagctc gttcgatttc
     1621 ttgctgaacc ggactcggcg gccatctgcc tgagtccgta cacccaaccg cgtcggcttc
     1681 tgtgtcaccg gacacaggaa catcacgttc gaaatagcca acggggcttc cttctcgaga
     1741 atgccgccct gcttcacctt ctgattcggc ttggtgtgcc gcttaatcat gttcaatttc
     1801 tcgaccacga tcttccctgc cgtgcgatcc acggaaagca ccttcccgga cttgccgcga
     1861 tcgcgtcccg cgatcaccat caccgtatcg cccttccgaa tacgaatttt ctgcaacgcg
     1921 tccacgatta ccccttactt ggaatctggc tagagcactt ccggagccaa cgaaataatc
     1981 ttcatgaact tcttccagcg caactcgcgc gcaaccggac cgaaaatgcg tgtcccgatc
     2041 ggctcgccgt ccttgttgat gaggacgcag gcgtttcggt cgaacttgat ataggagccg
     2101 tcttcacggc gcacttcctt ggtcgtccgc acgatcacgg cgcggctgac atcgcccttc
     2161 ttcaccgtcg cctgaggaat cgcttctttg acggccacga cgatgatatc gccgagagag
     2221 gcgtagcgcc gcttcgaccc gcccagcacg tgaaagcaca tggcctgctt cgcaccggag
     2281 ttgtcggcca cgtccatata tgtgtaattc tgaatcatga tctctcttat gtcctcaacg
     2341 agtaggccct gaagaactac tcagctcgcc ctttttccaa gacttcgacc acacgccaat
     2401 gcttgtcctt gctaatcggc cgggtttcga tcatccgcac gcgatcgcca acattgcaga
     2461 cgttctgctc atcgtgcgcc ttgaatttgg tcatccggcg cagtaccttc cggtagatag
     2521 ggtgcacgac cgaacgctcc accgccacga tgacggtctt gttcatctta ttgctcacga
     2581 cacgcccgac ccactcgcgc cgtttgactt ccgtctctgc catcatccca accctttccc
     2641 cttacgaagc cttcttctga tcggcttgtt ccacttcgcg cagaatggtt tttacctggg
     2701 caatcgtccg tttcgttttc cggatctgca tgggattttc cagtctcccg gttcccaatt
     2761 gaaaacgaag attaaagagc tcctgcgtca attgtttggc cttttccgag agttccgcgc
     2821 ccgtcatctg tcgcaattct tttagttcca tctcaagtct cgcccttcag ctatccggcc
     2881 aacaaactta gaattctccg cgagtcacac atttggtcgc gatcggcaac ttgtgggacg
     2941 caagccgaaa cgcttcgcga gcgatttccg gcgtcacgcc atccatctcg tacagaatcc
     3001 ttcccggctt gacgactgca acccaatact cgggatttcc cttcccttta cccattcgag
     3061 tttcagccgg cttcttggtg atcggcttgt ccgggaaaat cctcgtccac acctgaccac
     3121 cgcgtttcac atatcgcgta atggcaatac gcgccgcttc gatctgccga ctggtgaccc
     3181 aaccgggctc cagcgctttc aatccaaatt cgccgagtgt aatgtggccg ccacgataag
     3241 ccttgccacg catccggccc ttcatcattt ttcgaaactt aacttttttc ggtgctaaca
     3301 cggttccctc ttcgcttacc caagccgccg ttcaaacgcc gcttcgggct tgagcggctg
     3361 tgcggggagg agttctccct tgtagatcca cgttttgacg ccaatttgac ccatcgtggt
     3421 gtgggcttcc gcaaacccgt agtcaatttc agcacgcagg gtatgcaggg gcacacggcc
     3481 ttcccgatac cactcggtcc gcgcgatttc cgcgccgccg agccgcccag cgaccatgat
     3541 cttgatgcct tgcgcgccga gacgcagcgc cgattgcacg cttcgtttca tggcccgacg
     3601 aaatgccacc cgcttttcca gctgagtcgc gacgttctcg ctcaccagct gcgcgtctag
     3661 ttctggcttc ttgatctctt tgacggtgat gtagacctgc ccgccatact gcttctccaa
     3721 ttcggctttc agcttgtcga cctcggcacc cttccgtccg atgatgatac ccggccgcgc
     3781 cgtatggatg atgacgcgcg tctgatctcc ggaccgttca atctccactt tggccacacc
     3841 ggcgtggtag agcttctgct tcaccacctt ccggatcttg atgtcctgat ggagcaactt
     3901 tgcgtagtct ttccccgcat accagcggga actccacgta tagttgtacc caagccgata
     3961 acctattgga tgtgtcttct gccccatacg cgcatgcctc agttctgcgg cggctgactc
     4021 gcctctccgc tcttatctgc taaagcctat gacttccccg tgacgcccgg ggcttccaca
     4081 acaacggtga tatggctcgt tcgcttctga atcgcgttgg cacgtcccat cgagcgggcg
     4141 cgaaaacgct tatagatcgg gccacaattg acgaacgcct ttgacacccg catcgactcg
     4201 ctgtccccca actccttttg ttcggcattc gcgacggccg aacggagaat cttttcaacc
     4261 acgcgggctg catgacgcgg gaggtgcttc aacatggcca acgccatggg cacttgctgc
     4321 cctcggatca tatcgaccac gggacgcgcc ttgcggggag cgacgcgaac gaatcggagt
     4381 actgctcgtg cttctgccat gtcctaatcc cttacgtacg gatgccggca tccattgcct
     4441 gaaaactcgg tttacttgag cgcgaccgcc ttttcggtct tcgcctgtcc atgcccttta
     4501 aaaaatcgcg tcggggcaaa ctcgcccagc ttgtgtccaa ccatattttc cgtcacgaac
     4561 accgggataa acttcttgcc gttatgaacg gcaaacgtat ggccgatcat atccggcacc
     4621 acagtcgagc gacgcgacca ggtcttaatg attttccgat cctttgtctg attcatctgc
     4681 tccactttct tcagcagatg atcgtctaca aacgggccct tgctaatcga tctaggcatt
     4741 gcgcactcct gacttgcggc gggcgatgat gaacttatcc gtcgccttgt tgttgcgcgt
     4801 tttataaccc ttcgtcggta aaccccaggg agacacgggg tgcgggttac cctgtccgga
     4861 cttaccttcg ccaccgccgt gtgggtggtc gaccgggttc atcacgacgc cgcgcacgtg
     4921 aggacgacgg cccagccagc gcgtacgccc cgccttcccg acagtgatgt tttcatgatc
     4981 aagattgccg acctgtccga ctgtcgccat gcaagaggcc aggatccggc gcatttcacc
     5041 ggatttaagc cgaacctgga cataatcccc atcgcggccc atcacttgcg cgaaccctcc
     5101 ggcactccgg atgagttggc cgcccttacc gggcttgagt tcaatattat ggattgtcgt
     5161 acctagcggc atgttcgcga gcggcagtga attaccggtc cgaacttctg catcgatgcc
     5221 ggactgcacc acgtctccca cctgcaaccc cacaggcgca agaatgtatc gtttttcgcc
     5281 gtccttataa ttcaatagtg cgatcctggc tgagcgattc ggatcgtact cgatcgccgc
     5341 aaccttggcg gagatcccta ccttctcccg gcggaaatcg atcttgcgat agagccggcg
     5401 gtgccccgcc cctcggaatc gcacggtcgt acgtccgtcg ttgttgcgac cagcggtccg
     5461 cagatgaaac gacgtgagcg atttttcagg cttcttcttc gtcagttcgt cggtcgtcac
     5521 gatcgtcatc ccgcgtcggc ctgacgtcgt cggcttatag gcttttaatc ccatgcgctt
     5581 ctcttccttc ttactattct cacccgccca ccctgacggc gccgagacgc cgcgtgaccc
     5641 aggctatgcg ctctcgtaca gctcgagctt ctcgcctgcc ttcaacttca cgaaggcttt
     5701 cttccaatca gagcgttttc cggagaaccg gcccaagcgc ttgattttgc cttgcacgtt
     5761 caagacattg acccgatcga ccttcacctt caacaatgct tccaccgctt gcttgatctg
     5821 cactcgattg gcatccggat gaaccaagaa actgaccgtg ttattggctt cacgcatcgc
     5881 cgtgactttt tcagtcaaca acggttgaat gagcacactg tggagatcga gcttcatgcc
     5941 cacacctcct taactcgcgc cagatcccgt tcgggaatca cgatcatgcc cgcacgcaca
     6001 acgtcataga cattgagacg atcgggggtc accacgctga ctttcgctag attgcgagct
     6061 gcttgcgtga gtcccgtctg tccttcaccg gcaacgatta gaacctgccc gccggcgccg
     6121 aattgaccca gcgccttggc tagcaacttg gttttgggct gctccagcga caagctggag
     6181 acaacaacga gatgcccgcc ggcaactttc gcggacaaag cgctctgcag cgccgcgcga
     6241 tattttttct tcggcatgct gaacgcatag ctcctcggct tcggaccgaa taccgagccg
     6301 ccatgacgcc atacgggaga gcgcacggag ccagcccgcg cgcgaccggt atgcttctgc
     6361 ttccagggct tttttcctga accggcaact tcaccacggc gcaacgtcga cgcggtcccc
     6421 tgccgttcac aggcacgctg catgatcact gcttcgtgca cgagcgccgt atgcggcgcg
     6481 cagccgaata cttcggcagg caattccgcc gatccaacct tccgcctgtt caaatccacg
     6541 acatcaaccg tcggcatggc cctatccctt cttcgacttt ctgacgacca gcagtccatt
     6601 cgccgctccg ggaaccgccc cgctgacgaa cagaagattc tcatccggtc tggactcaat
     6661 caccttcaaa cgctgggttg tcactcgctc cgctcccata tggccaggca aggtttttcc
     6721 cttccagaca cgggaaggaa acgaactggc gccaatcgaa cccggcgccc ggtgaaacat
     6781 ggacccgtgc gattctggac cacctgcata atggtggcgc ttcacgacac cctgaaatcc
     6841 cttgcccttt gatacgccaa tgacatcgac ccaatcgcct tttttgaaca ggtcggcttt
     6901 gatcacctga ccgaccgcca catctccgtc cttcttgaac tccttcagaa tccggctggc
     6961 aggggcctcg tgcttcttca gatgacccag ttccgctttg gacagcttgc ggtctttcac
     7021 ttcgccaaac gacagctgca ccgcttcata cccgtcacgt tctttggtct tcaccatcac
     7081 gacgcgacac ggcccagcct cgatcaccgt gaccggagcc agacgagccc cgtccaacat
     7141 ctgcgtcatg ccaagttttt taccgagtaa cccgttcgtc atacagcacg ttccttatcg
     7201 gttctcagcg tcagagctta atttcgacgt cgacaccggc cgccaaattg agcttcatga
     7261 gggagtccat ggtctccggc gtcggctcca tgatgtccat caagcgcttg tgtgtcctga
     7321 tttcaaattg ctcccgcgcc ttcttgtccg cgtgcgtcgc agcctgcacg gtaaatttct
     7381 cgattctcgt cggcaaggga atcggtccga ctactttcgc accgctgcgc cggacggtct
     7441 cgacgatctc ggcgactgac tggtccagca cacgataatc gaaccctcgc aacctgattc
     7501 tgattcgttg atcaaccttc acacatcacc cctaggctgt ccgtggacag cacgctgcgg
     7561 ttaggctaag atttccgtga ccacgccgga gccgaccgtc ttgccgccct cgcgcaccgc
     7621 gaagcgcagc ccctgatcca tcgcaatcgg gctgatcaac tcggccgtca cactcacatt
     7681 gtcccccggc atcaccatct ccacccccgg attgagcgac acaatgcccg tcacatcggt
     7741 tgtccggaag tagaactgcg gccggtaccc attgaagaac ggcgtatgcc gccccccttc
     7801 ttccttcgtc agcacgtaga tctcggcttt gaacttcgtg tgcggcgtaa tgctcttcgg
     7861 cttggccagc accattccgc gctccacgtc ctctttcttc gtcccacgca gcagcacccc
     7921 gatgttgtcc cccgcctggc cctcatcgag caccttgcgg aacatttcca ccccggtcac
     7981 caccgtcgtc tgcgtcggct tcaaccccac gatctcgatt tcgtccccca ccttcacgat
     8041 cccgcgc
//
LOCUS       DATIFR010000164         1498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1065316, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000164 DATIFR010000000
VERSION     DATIFR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065316"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1308)
                     /locus_tag="VLD60_09745"
     CDS             complement(229..1308)
                     /locus_tag="VLD60_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSB45284.1"
                     /translation="MRGQRGLFDVEDRLKRLSDLGDQLEAFRAAVDFEIFRPDLETAL
                     AYSDGAQGGRPPFDPVMMFKILIIQATNNLSDERAEFLINDRLSFMRFLGLGLEDRVP
                     DARTIWLFREKLTTAGAIKGLFERFDVMLRQAGYIAMSGQIVDASLIVAPRQRNTDDE
                     KQAIKEGRIPQDWKDKPAKLRQKDRDARWTVKFTKAKPREDGSMPPVDIAIPLFGYQN
                     HISIDRGFGFIRQWAATDAAAYEGQRLREGLLDKTNTASGVWADTAYRSAVNETFLAR
                     NGFFSHIHRKKPKGRAMPETIRRANNAKSKIRSRVEHVFAEQKDRMGLFIRTIGIARA
                     TTKIGMANLVYNIKRLLFLRRTAVA"
BASE COUNT          280 a          454 c          421 g          343 t
ORIGIN      
        1 cttgctcttt ttggccagtt gctgcagcat catgccatca aaatcatcac gcaggccgac
       61 tgctcttgcc atggcgaacc tccttcgttc gcccttttga atctggacgg ttctattaac
      121 gcacggattt ttccggcctg agcatctttg aagggctttg actgagattt tctcgatcgg
      181 gtggatgcct acggtctgag tccggcggcc ttttgaagac ggatgccctc atgcgaccgc
      241 cgtccgcctg aggaacagca agcgtttgat gttgtagacg aggttagcca ttccgatttt
      301 ggtcgtcgcc cgggcgatcc cgatggttcg gataaacagg cccatccggt ccttctgctc
      361 agcgaacaca tgctcgacgc gcgagcggat tttcgatttt gcgttattgg cacgtcggat
      421 cgtctcgggc atggctcggc ccttcggctt cttgcgatgg atgtggctga agaagccatt
      481 ccttgccagg aacgtctcgt tgaccgccga ccggtaggct gtatcggccc ataccccgct
      541 cgccgtattg gtcttgtcga gcaagccttc gcgcaaacgc tggccctcat aggcagccgc
      601 atctgtggcg gcccattgcc ggatgaagcc aaagccgcgg tcgatcgaga tatggttctg
      661 gtagccgaac agcggaatcg cgatatcgac cggtggcatt gagccatcct cccgtggctt
      721 ggccttggtg aacttcaccg tccagcgcgc atcgcggtct ttctggcgca gcttggccgg
      781 cttgtccttc caatcctgcg gaatgcggcc ctccttgatt gcctgtttct catcgtccgt
      841 attgcgttgg cgcggagcga caatcaggct ggcatcgacg atctgccccg acatggcgat
      901 gtagcctgcc tgccgcagca tcacgtcgaa tcgctcaaac agccccttga tggctccggc
      961 cgtggtgagc ttctcccgaa acagccagat ggtgcgggcg tccggcacac ggtcctcaag
     1021 ccccagccct agaaaccgca tgaatgacag tcggtcattg atgagaaact cggcgcgctc
     1081 gtcggagaga ttattcgtcg cctggataat caggatcttg aacatcatca ccggatcgaa
     1141 gggcggtcgc ccgccttgcg cgccgtccga gtaggcgagc gctgtctcca ggtctggccg
     1201 gaatatctca aaatccactg ctgcacggaa tgcttcgagc tgatcaccga gatcgctcag
     1261 ccgcttcaga cggtcttcaa cgtcgaaaag tcccctttgt cctcgcatgg cttcggctcc
     1321 aatccaccga atccaatgga atcagaaaag ctaccaacct gccaggtttt tagaactgtc
     1381 cagattcagc agggtggcgc tcgccagtgt ccattgtaca gaccgggtca cgaccgcgag
     1441 tgaaatccac gaggcggcgg caactgcaag gctgacaatg agcccgcgcg tttgcgcc
//
LOCUS       DATIFR010000165         1598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_490788, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000165 DATIFR010000000
VERSION     DATIFR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490788"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VLD60_09750"
     CDS             <1..1186
                     /locus_tag="VLD60_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSB45285.1"
                     /translation="DALKASEVRYRRLFETAKDGILILDADTGRIIDANPFLRDLLGY
                     SHDELLGKMLWEIGPFRDIAASRDAFRKLQKKEYIRYDNLPLETKGHRHKHVEFISNV
                     YLVDGTRVIQCNIRDITVRHQAEVALVNLRKELEKRVEERTAELLTANKLMKKMLDEG
                     KRSEEKLLKSRERLRNLSGRLQSMLEEERMRISREIHDELGQALTALKLDLSLIRRRL
                     TSDGLDEPSAKVHEIERSVNGIIRTVRKISTELRPGILDELGVAAAIEWMVKDFQKRT
                     GITCKVSLQEMEKISDPVRSTAIFRITQEALTNVMRHAAASKVKVNLEKKDNTLVLEV
                     IDNGIGIMEERIFDSKSFGLIGIRERVLLLGGEAVISGKQGEGTMVRVTLPMGKGENP
                     GA"
     gene            1179..>1598
                     /locus_tag="VLD60_09755"
     CDS             1179..>1598
                     /locus_tag="VLD60_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45286.1"
                     /translation="MLRILIADDHEVVRKGLVRVLEEALQPIKIDEARNGQEAVSKVW
                     KSEYDLVVLDLKMPGKGGLEALKEIKDQRPKLPVMILSMHPEEQFAIRAMRAGASGYL
                     TKECAGDELILAIRKALKGEKYISGSLSQILASELDRD"
BASE COUNT          427 a          381 c          495 g          295 t
ORIGIN      
        1 ggacgcgctg aaggcttccg aagttcgcta ccggcgactc ttcgagacgg caaaagacgg
       61 catcctcatc ctggatgcgg acacgggacg gatcattgac gcaaacccct tcctccggga
      121 tctgctcggg tactctcacg acgagctgct cggcaagatg ctctgggaaa tcggaccatt
      181 cagggatatc gccgccagcc gggacgcttt caggaaattg cagaagaagg agtatatccg
      241 ttacgacaac ctgccgctgg aaaccaaagg gcaccggcat aagcatgtgg aattcatcag
      301 caatgtctac ctggtggatg gcactagagt gattcaatgc aatattcgcg acatcaccgt
      361 acgacatcaa gcagaggtgg ccttggtgaa cctccgcaaa gagcttgaaa aacgcgtgga
      421 ggagcggacg gcagagctgt tgacggcaaa caagctcatg aagaagatgc tcgacgaggg
      481 gaagcggtca gaggagaagc tcttgaaatc ccgagagcgg ttgcgcaacc tctcggggcg
      541 tctgcaatcc atgcttgagg aagaaaggat gcgcatctca cgcgaaattc acgacgagct
      601 cgggcaggcg ttgacggctt tgaaattgga cctctcgttg ataagaagaa ggttgacctc
      661 cgacggactt gacgagccgt ccgcgaaagt ccatgaaatc gaacggtccg ttaacggcat
      721 tatccggacc gtgcgaaaga tttcgaccga gctgaggccc ggaatcctgg atgaactcgg
      781 ggtggccgcc gctatcgaat ggatggtgaa ggatttccag aaacggacag ggataacctg
      841 caaagtttcg ctacaggaaa tggaaaagat atccgaccct gtccgctcca cggcgatctt
      901 ccgcatcact caggaggctc tgacgaatgt catgcgccat gccgcggcat cgaaggtgaa
      961 agtgaacttg gagaagaaag acaatacact ggtattagag gtgatcgaca acggaatcgg
     1021 gattatggag gaacggattt ttgactcgaa gtcgttcggc ctgatcggga ttcgggaacg
     1081 agtcctgctc ctcggagggg aagccgtaat cagtgggaaa caaggcgaag ggaccatggt
     1141 aagagtgact cttcccatgg gaaaagggga aaacccgggt gcttagaata ttgatcgcgg
     1201 atgatcatga agtcgtacga aaaggattgg tgagggttct tgaggaggcc ctccagccca
     1261 tcaagatcga tgaagcgaga aacggacagg aagcggtgag caaggtctgg aagagcgaat
     1321 acgacctggt cgtgctggac ctgaagatgc ccggcaaggg cggattggag gctttgaagg
     1381 agatcaaaga tcaacggccg aagcttcccg tcatgatcct cagcatgcac ccggaggaac
     1441 agtttgcgat acgggccatg cgcgctggcg cctccggata cctcaccaag gaatgtgcgg
     1501 gtgacgagct gatcttggcg atacggaaag cactgaaagg cgagaaatac atcagtggct
     1561 cgctctccca gatcctggcg agtgaattgg atagggac
//
LOCUS       DATIFR010000166         6080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1638343, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000166 DATIFR010000000
VERSION     DATIFR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638343"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VLD60_09760"
     CDS             complement(<1..1173)
                     /locus_tag="VLD60_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45287.1"
                     /translation="MTRSSKRIEAALHVVGTHSMKAIGIALLCATLLLSCDSIRSLIK
                     PGGTPGTVVDEARLGNRSVASFPAADEDYFADMEGGAKLSPEEAKGRNTWIVWSGGND
                     RFWDELSKLSFGTLDFLKTLSSHPKLKFSRDTRWYYLGLVNEPCFEKATGPDPNRFGL
                     WLDKRVVSPGCPADPFENETKYPGVKIGARGKNLPVGSFYGYATGIVGLRLFPNPAFD
                     EEAAKKWDPKRYYEDPTYYLSKDLVRPYRVGMSCAFCHVGPNPAKPPENPEHPEWKHL
                     SSNVGAQYFWIDRIFSWEADETSFPYQIFRTSRPGALDTSLISTDNVNNPRTMNAVYH
                     LGPRLEQAKRWGQEKLAGGNLDNKQLNDYVASGPLTTFFHAPDTVWTPRVLKDGSDS"
     gene            complement(2027..2734)
                     /locus_tag="VLD60_09765"
     CDS             complement(2027..2734)
                     /locus_tag="VLD60_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSB45288.1"
                     /translation="MKTLSDTPLVLNERKPAQTMPQHPSTGVVLFNSIGQLLFMNHEA
                     QLFIRQLQPPSNRENGTCLIPEDIHTIVRDLIDRLMHCDHPKDCEAIQVERLCFAADQ
                     RLLLRGLCIPDEPLARNSRFLIILEKLNQKLECPDSNIQQRYHLTEREQMVIIYLMLG
                     FTNKEIANRLNLSEYTVKEHLKRIMHKTKTTTRTGLLARMIFPTPQGAIPGLLSEPLG
                     AVASPASLRLKPLNAIA"
     gene            3156..3284
                     /locus_tag="VLD60_09770"
     CDS             3156..3284
                     /locus_tag="VLD60_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45289.1"
                     /translation="MKRTEIVLPGSCETALLERFLKAEAMALWSVRAAQAKEECFL"
     gene            3340..3633
                     /locus_tag="VLD60_09775"
     CDS             3340..3633
                     /locus_tag="VLD60_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSB45290.1"
                     /translation="MKRTAVSTLKATLSACLARVKAGGEVVVMERGKPIAKLVPLMKD
                     NGSTIGQMDELARAGLVRVGRGKLPAGFWKLPRPKDRKRRALRALLAERAEGR"
     gene            3801..4058
                     /locus_tag="VLD60_09780"
     CDS             3801..4058
                     /locus_tag="VLD60_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45291.1"
                     /translation="MEEEQAQTILRALMSSWTEVEPSMLVREQAGRVLRLHPLRAADA
                     LQLAAALVWCQGDPIHHALISLDHRLREAARGEGFQILPRR"
     gene            4106..4687
                     /locus_tag="VLD60_09785"
     CDS             4106..4687
                     /locus_tag="VLD60_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSB45292.1"
                     /translation="MPVHLSSSCESDLLERFLKAEAMALWSVRAAQAKDVPPGVLQFL
                     RRHEEEEAKHLQEFEVRLGVRSHAREKLPRVPSQWWALVVQLYGYETLGLEFAKLLVQ
                     VQPDLRSILEDEEGHVGFFEQEVGKVLAQGGDSAEGARQAAQAWWRRLPPTVDRYLEG
                     ESLAPFRLDLRHLILDSIRARFVGVGLLNSLTV"
     gene            4703..5233
                     /locus_tag="VLD60_09790"
     CDS             4703..5233
                     /locus_tag="VLD60_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSB45293.1"
                     /translation="MDCILVRHGIAVERDEWEGTDADRPLTDRGAKRVEQIAAGLKWL
                     DVQPTHIFSSPLVRAVETARILQTTFALRASIRRVEELLPGAEPERLLVLLHDLPGKS
                     CVVITGHEPHLSAAASVMLTGKSSQAFPFKKGGACLIEMPVPAKPGHGILRWWMGPAQ
                     LRAVGKKQAKVEAKEQ"
     gene            complement(5255..5986)
                     /locus_tag="VLD60_09795"
     CDS             complement(5255..5986)
                     /locus_tag="VLD60_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HSB45294.1"
                     /translation="MVDAHYFGDGLTYLNPSDLKGKLIAIEGTDGVGRSTHIELLQEW
                     LEVQGYGVITTGWTRSNLMSKTIEMAKEGNILDRWSLSLLYATDFADRLEHQIIPALR
                     SGFIVLADRYIYTAFARDYVRSNDRKWIRDVFGFALVPDLVCYLRIDVETLVLRVIET
                     KAMNYWESGMDLRLGNDLYDSFKKYQSLLIEEFDKMTEEFKFEVVDARKSPEEIQDEL
                     RTKIQQLLTKEGLAQLSIPAAAERA"
BASE COUNT         1210 a         1559 c         1883 g         1428 t
ORIGIN      
        1 ggaatcagat ccgtctttca gcacccgagg ggtccacacc gtgtccgggg catgaaagaa
       61 tgtcgtcagc ggcccgctcg cgacatagtc gttgagctgt ttgttgtcca agttgccgcc
      121 ggccaatttc tcttgccccc atcgcttggc ttgctcgagg cgcgggccga gatggtacac
      181 ggcattcatg gtgcgggggt tgttgacatt gtcggtcgag atcagcgagg tatcaagcgc
      241 gcccggacgc gatgtgcgga aaatctggta ggggaagctg gtctcgtcgg cttcccatga
      301 aaagatgcga tcgatccaaa aatactgggc gccaacgttc gagctcaagt gcttccattc
      361 cggatgctcc ggattctcag gaggctttgc cgggttcggc ccgacgtgac agaacgcgca
      421 ggacatgccg acgcgatagg gcctcacgag atccttcgag aggtagtagg tcggatcttc
      481 gtaatagcgc ttcgggtccc atttcttggc ggcttcttcg tcgaaggcgg gattggggaa
      541 gaggcgtaac cccacaatac cggtcgcgta gccgtagaac gatcccaccg gcagattctt
      601 gccgcgggcg ccgatcttga cgccggggta ttttgtttca ttttcaaacg ggtcggctgg
      661 acagcccgga ctgacgacac gcttgtcgag ccagagtcca aatcgattcg gatcgggccc
      721 ggttgccttt tcaaagcaag gctcattgac caatccaagg tagtaccagc gggtatcgcg
      781 gctgaatttc aatttcgggt gcgacgaaag ggtctttaga aaatcgagcg tcccgaagct
      841 gagtttcgaa agctcgtccc agaaacggtc gttaccaccg ctccacacga tccacgtgtt
      901 gcggcctttc gcttcctcgg gggataactt ggcaccgccc tccatgtcgg caaaataatc
      961 ctcatctgcg gctgggaacg atgcgacgga ccggttccct agacgcgctt catcgacaac
     1021 cgttcctggc gtgccgcccg gttttatgag agatcgaatc gagtcgcagc tgagaagcag
     1081 cgtggcacag agcagtgcaa tgcctatggc cttcatcgaa tgtgttccaa caacgtgcaa
     1141 tgctgcttcg attcgtttac ttgaacgcgt catcgaactc tctccccgaa cctgacaatg
     1201 gtgtcgggag gctgtcctcg ttgaacgctt cctttattag aagaaccgga cccttcgtca
     1261 gggggaggac atgaaagggt gtcgcgaacc aattcatggt catcttgctg tctggattgg
     1321 attgaagacg gttgcagagg gggagcggca ggatggtggt gtctgcaatc ccggaaatca
     1381 ctcacaccac ctcatcatca ttgcccccat ggacagaatt cagaggagag aaccccgaga
     1441 attccagggt tcccgcgccc cctaattatt cctgttggtg catttccgca ataacccttt
     1501 cgaatagggc catatagaaa tagatgttaa ttattcttct ccaatatctt gggggcaatg
     1561 ggtcgcaaag cccaattgtt acccgtcgcg gtctcaatac atacaaggta tcctgacgat
     1621 ctctccctga gccatcaatc cggttggctg ttttcgttca ggaataagta ggccgcgcta
     1681 ccagtgatgg tggaagcgcg gcctggttgg cgtggaggca actcagcaga actctggaag
     1741 ggtcaggacc agaatctgct tcgaggggga ggctatcgct tgctagccga ttcccctagg
     1801 tcggctccac accgaagtca tttctcattc gaggccctga gtcagacctc gtttccctgg
     1861 tctctccagc agtcaccctg cttcatcgtc agacgcttca cctggcgcgc atctcagtct
     1921 tgccagatgc gggtcacgat caggtagatg aagacggcta agagagtgtt gcccacgacg
     1981 agaagaatgg cgaaatccat ggccaattcc ttttctcctg gacgtgttag gcgatcgcat
     2041 tgagcggctt cagcctcaag gaggccggtg acgccactgc ccccaacggt tcggacagga
     2101 gtccggggat ggcgccttgc ggcgtcggga agatcatgcg ggccaacaat cctgtgcggg
     2161 ttgtggtttt ggttttgtgc atgatccgct tgagatgctc tttgacggtg tactcgctga
     2221 ggttgagtcg attcgcgatc tccttgttgg taaagccgag catgaggtaa atgatcacca
     2281 tctgttcacg ctcggtcagg tgatagcgtt gctggatgtt actgtccggg cactcgagtt
     2341 tctggttgag tttttccaga atgatcagga agcggctgtt cctggcgagc ggttcatcgg
     2401 ggatgcagag cccgcggagt aacagccgtt ggtccgctgc gaagcacagg cgctcgactt
     2461 ggatggcttc gcagtctttg gggtggtcgc aatgcatcag ccgatcaatg agatcgcgca
     2521 cgatcgtgtg gatatcttcc gggatgaggc aggtgccgtt ttccctgttt gacggcggtt
     2581 gcagttgcct gatgaacagc tgggcttcgt ggttcatgaa caggagctgc ccaatgctgt
     2641 tgaagaggac gactcctgtt gagggatgtt gcggcatggt ctgtgccggc tttcgctcat
     2701 tcagaaccaa gggcgtgtcg ctcagcgttt tcatcggcgt ccccccattc gtttgtgatg
     2761 acggcagtat gatttggagt gaaaaagcat ctatttatgt cccacccact ggatttgcat
     2821 gggctttcgt tcacaagaat ttatcctaca gctctgcaat cgacaggcca gaaccattta
     2881 cagttggtga ttcgaatgaa aaagcgagga aacgcttgtg ctggattgat atttttcgtg
     2941 ttcgtctctt tccgtcattt tgctctagca gatcgctcac cttggatggt ctgagtcgaa
     3001 aacgatcctg attgaatcag aaacgatcca aataatgagc cttttctatg gtttaccggt
     3061 attaatacga ggggcttcct tttgagccac gccggcctca tcgaacccgg agttggctgt
     3121 tggttcttgc tccaggtttt gggtacactc gctcgatgaa acggacggag atcgtgctgc
     3181 ctggttcgtg cgagacagcc ttgctcgaac ggtttctcaa agccgaggcg atggccttgt
     3241 ggtcggtccg ggcggcccag gcaaaggagg agtgtttctt gtgagacttc tcccaaggga
     3301 ctatattggt cacatgcacg agatcaagga ggtcttttga tgaaacgcac ggcggtttcg
     3361 acattgaagg ccacgttgag cgcctgcttg gcaagagtaa aggccggcgg tgaggtggtt
     3421 gtcatggagc ggggcaagcc cattgccaaa cttgtgcctt tgatgaagga caatggcagc
     3481 acgattggtc aaatggacga gcttgcgcgt gccggattgg ttcgtgtcgg taggggcaag
     3541 ctgccggctg gattttggaa acttccacgc cccaaggatc gtaagcggcg cgcgctccgg
     3601 gcgctccttg cagagcgggc cgaaggacga tgaagttttg agatgcctct gccttgatcc
     3661 cattgtgtct gcaggagcag caaagcagcg ctctgaggaa gctcgctcaa gaagacgaat
     3721 cgattgtggc ctggtggggt tcgccggtcg agtgtctatc tgcattggca cgcctgagaa
     3781 gagaggatgg gctcagtgcc gtggaagaag aacaggcgca aacgatatta cgggctctca
     3841 tgagcagctg gacggaggtg gagccaagca tgctggtgcg ggaacaagca ggacgggtgc
     3901 tgcgcctcca tccattgcgg gcagcagacg cgctgcagtt agccgcagct ctggtgtggt
     3961 gtcagggaga tcccattcat catgccctca tcagtctcga ccaccggttg cgggaagctg
     4021 cacgagggga gggattccaa atccttccac gtaggtaaga caatcgggtt gcagactgat
     4081 ctctattcct tattacaatt agaccatgcc ggttcatctt tcttcctctt gcgagtctga
     4141 tttgctcgaa cggtttctca aagccgaggc gatggctttg tggtcggttc gggcggctca
     4201 ggcgaaggac gttccgcccg gtgtgcttca atttctgcgg cggcatgagg aggaagaagc
     4261 gaagcatctt caggagtttg aagtgcggct gggggtgcgg tcgcatgcca gggagaagct
     4321 tccccgagtg cccagccaat ggtgggcgtt ggtcgttcag ctctacgggt atgagacgct
     4381 ggggctcgag ttcgccaaat tgctggtgca ggtgcagccc gacttgcgat ccatccttga
     4441 ggatgaagag gggcatgtag ggttttttga gcaggaggtc gggaaggtgc tcgcgcaggg
     4501 cggcgattcg gccgagggag cgaggcaggc tgctcaggcc tggtggcggc ggttaccgcc
     4561 gactgtggat cgctatttgg aaggagagtc gctggcgccc tttcgattgg acctgcgaca
     4621 cctgatcttg gattcgattc gagcccgctt cgttggggtc gggctgctga attctctgac
     4681 ggtgtgagcg aaaggagtgt cgatggattg cattttggtt cggcatggta ttgccgttga
     4741 gcgggatgaa tgggaaggaa cggatgccga tcgcccgctg accgaccgag gcgcgaaacg
     4801 tgtcgaacag attgcggccg gattgaaatg gctggatgtg cagccgaccc atattttctc
     4861 gagcccgctc gttcgtgcgg tcgaaacggc cagaattctt cagacgacgt tcgccctgcg
     4921 tgcttcgatc cggcgggtgg aggaactgct gcctggtgcg gagccggaac gactccttgt
     4981 gctgctgcac gatttgcccg gcaaatcctg cgtggtgatc accggccatg aaccgcattt
     5041 gagtgcggcg gccagcgtga tgttgacggg aaagtcctcg caggcctttc ccttcaagaa
     5101 gggcggcgcc tgcctgatcg agatgcctgt gccggcgaag ccgggccatg ggatcttgcg
     5161 ttggtggatg gggccggcgc agttgcgggc tgtggggaaa aaacaggcga aggtagaagc
     5221 caaagagcag tgagggcaag gctgaggcga actcttacgc tcgttcagcc gctgccggga
     5281 tcgatagctg ggcgagtcct tccttggtca ggagctgctg gatcttggtc cgcaattcgt
     5341 cctggatttc ttcgggggat ttgcgggcgt ccacgacctc gaacttaaac tcctccgtca
     5401 ttttgtcgaa ctcttcgatg aggagcgact ggtatttttt gaagctgtcg tagagatcgt
     5461 tccccagccg caggtccatg ccggattccc aatagttcat cgccttggtt tcgatgaccc
     5521 gcaggacgag cgtttccacg tcgatgcgaa ggtagcagac cagatcaggc accagcgcaa
     5581 agccgaagac gtctctgatc catttgcggt cgttgctgcg gacatagtcc cgcgcgaagg
     5641 cggtgtagat gtagcggtcg gcgagcacga tgaagccgga tcgcagcgcg ggaatgatct
     5701 gatgctccag gcgatcggca aaatccgtgg cgtagagcag gctgagggac caccggtcga
     5761 gaatgttgcc ctctttggcc atttcgatcg tcttggacat gaggttggac ctggtccagc
     5821 cggtggtgat cactccatag ccctgcactt ccagccattc ctggagtaat tcaatatggg
     5881 tcgaccggcc gaccccgtcc gtgccctcga tggcaatcag cttgcctttg aggtcactcg
     5941 gatttaggta cgtcaaaccg tcgccaaaat aatgagcgtc gaccatgggt tccccgcctg
     6001 ggtttgtaat ctgtgagcgc ttcgtcgacg agacggcgca tgctttcctg ctgggccgcg
     6061 atgtctttcg tggcatccat
//
LOCUS       DATIFR010000167         1466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_299237, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000167 DATIFR010000000
VERSION     DATIFR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299237"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="tyrS"
                     /locus_tag="VLD60_09800"
     CDS             complement(<1..307)
                     /gene="tyrS"
                     /locus_tag="VLD60_09800"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSB45295.1"
                     /translation="MSELVRQLDLILRGAVEVIHQAELESKLTRALKEHRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHQVIFLIGDFTGMIGDPTGVSETRKALTKEQVQ
                     "
     gene            complement(304..783)
                     /locus_tag="VLD60_09805"
     CDS             complement(304..783)
                     /locus_tag="VLD60_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSB45296.1"
                     /translation="MVSSLLLKLVMLAATMSVVFWIGWSVPQSRYADAHRGGNAQANE
                     HSSVPLPPSEQSTAVTETSLAGFSNTPRPMQVPSRSLLATLDLNRASEQELEALPGIG
                     PVLAERIVEYRHARGTFHDVEQLRKVKGIGKKKFERLRTLVHVAPAKAPVREGREAT"
     gene            complement(783..>1466)
                     /locus_tag="VLD60_09810"
     CDS             complement(783..>1466)
                     /locus_tag="VLD60_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB45297.1"
                     /translation="VRCKDGTSFEVEKLLVSVGRGFNSRGIGLDRAGVQTGRRGDILV
                     NERLETNVPGIYAIGDVTGKAMLAHVASAQGKVAVENIMGHESMIRYDVIPAGIFTLP
                     EIGRVGLTEAQARERAEANGEEPTTSLKIGRFRYAGLGKAQATGEITGFWKVISEASS
                     GRILGVHMLGAHAADLIHEAALAMQVGATATQVSEMIHAHPTFAEGFMEAAEDVDGKA
                     IHQVRKRSS"
BASE COUNT          318 a          458 c          373 g          317 t
ORIGIN      
        1 cctgcacctg ctccttggtc aacgcttttc tcgtctctga cacgccggtg ggatcaccga
       61 tcatcccggt aaaatccccg atcaagaaaa tgacctggtg gccaagatcc tgaaagtgct
      121 tcagcttgtg aatcaggacc gtatgcccca agtgaagatc gggggccgtt ggatcgaaac
      181 cggctttcac acggagggga cgatgctcct tcaatgcgcg agtcagcttc gactcgagct
      241 cggcctggtg aatgacctcg acggcaccgc gaaggatcag atcaagttga cgaaccagct
      301 cgctcatgtc gcttctctgc cttcccgcac cggagccttc gccggagcca catggactaa
      361 cgtgcggagg cgttcgaatt tcttcttgcc aatccctttc acctttcgca actgttcgac
      421 atcatgaaag gtccctcgtg catgccgata ttcgacaatc cgctcggcga gaacagggcc
      481 gataccggga agcgcttcaa gctcctgctc acttgcccga ttcagatcaa gcgtcgcaag
      541 tagagaccgt gaaggaactt gcatcggacg cggtgtattc gagaagcccg cgagcgaagt
      601 ctccgtgacg gctgttgatt gttcactcgg cggtaatggc acagaagaat gttcgttcgc
      661 ttgcgcgttt cccccacgat gggcatcagc ataacgagac tgcggaacac tccagccaat
      721 ccagaagacg acactcatcg tcgctgcgag catcaccagt ttgaggagca aggaggagac
      781 catcagctgg acctcttccg aacctgatgt attgccttcc cgtccacatc ttcggcggct
      841 tccatgaacc cttcggcaaa tgtcggatgg gcatggatca tctcggaaac ttgtgtcgct
      901 gtggcgccaa cttgcatagc aagggctgcc tcatgaataa gatccgccgc atgggcgccg
      961 agcatatgga ccccgagaat tcgtccactg gatgcctcac tgatgacttt ccagaagcca
     1021 gtaatctctc cagtcgcctg cgccttgccc aaccccgcat aacgaaaccg accaatcttg
     1081 aggctggtag ttggctcttc tccattggct tccgcccgct cacgtgcttg ggcctcggtc
     1141 agtccgacac gaccgatctc gggcaaggta aaaatgccgg cgggaatgac atcatagcgg
     1201 atcatcgact cgtggcccat aatgttttcg accgccacct ttccctgcgc cgaagcgaca
     1261 tgggccaaca tcgcctttcc cgtgacatca ccgatcgcgt aaattcctgg cacatttgtt
     1321 tccaaccgtt cattcacgag gatatcgcca cgccgccctg tctggacacc ggctcgatcc
     1381 aaaccaatcc cccggctgtt gaatccgcgg cccaccgaga caagcagctt ctcgacttca
     1441 aagctcgtgc cgtctttgca acggac
//
LOCUS       DATIFR010000168         1566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1066173, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000168 DATIFR010000000
VERSION     DATIFR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066173"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..868
                     /locus_tag="VLD60_09815"
     CDS             158..868
                     /locus_tag="VLD60_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSB45298.1"
                     /translation="MAQEDTNIIDQPEVMKPRLVSIEISGKKYEVPEGITVIKALWYT
                     GQEVVRGAGCLGGFCGACATYYRTKDDPKVRTCLACQTAVQDGMSFTIMPPFPARKAL
                     YDIQSLKDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKGVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPVGLDTRIKEIRDGRFNDEWK
                     KILKMSEKELADHCATVK"
     gene            972..>1566
                     /locus_tag="VLD60_09820"
     CDS             972..>1566
                     /locus_tag="VLD60_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSB45299.1"
                     /translation="MDIHALQQIVHKTRDARRKQTIPKFSPADRDQLIHKHHPDYRAS
                     AYRPIRFGPNADEKTVVELAALLEGDSPIGDDIDLTPHYTTDVLVVGGGGAGCAAALH
                     AHHAGSKVILATKLRLGDSNSVMAQGGMQISVAPEDSPITHFIDTLKGGHMQNDHDLL
                     KVMVEEGPSIAKWLIELGVLFDRQADGNLHVKKGGGSS"
BASE COUNT          359 a          434 c          469 g          304 t
ORIGIN      
        1 aaggcgtcga aggcgatgcg tccgagcgcg accttgaacg cgatcatcga cgcgatcgcc
       61 tagcatacgg tagagagtaa ggggtaaagt gtgacgagag attagctggc tctgaccact
      121 aaccccttac ccttcacccc ttactggttc gaggaacatg gcacaagaag ataccaacat
      181 tattgatcag cccgaggtga tgaagcctcg gttggtctcg atcgaaatct ccggcaagaa
      241 atacgaagtg ccggaaggga tcaccgtcat caaggccctc tggtacacgg gccaggaagt
      301 cgtccgtggc gccggctgtc tcggcgggtt ctgcggcgct tgtgcaacct actaccggac
      361 caaggacgat ccaaaagtcc ggacctgcct ggcctgtcaa acggcggtac aggacgggat
      421 gtcgttcacg atcatgccgc cgtttccggc tcgcaaggcc ctctacgaca ttcagtcgtt
      481 gaaagatccg aagcaggacc tgttcaatct ctaccccgaa gcgccgctct gtcggaattg
      541 caatgcctgc acggaggcct gcccgcagaa gatcgacgtg cgtgaagggg tctggaaagc
      601 cgtgtttggc gacttcaaag gcgtctcgga aatgttcatg gattgtgtca tgtgcggcat
      661 gtgcacgccg gtctgcatcg ccgacattgc gccgaacctc gtcgcgctct acgtcagtcg
      721 cgctcaaggc gcgcatttca cggagaagcc ggtggggctg gatacgcgga tcaaggagat
      781 tcgggacggc agattcaacg acgagtggaa aaagatcctc aagatgagcg agaaagaact
      841 ggccgatcac tgtgcgacgg tgaaataact tcatgaggca aggggcgaga ggcgagaggg
      901 aagtcgagga tcctctgaaa ttattcgtgc agttcctcta gcctcgtgcc tctggcccta
      961 gcctgaaaat tatggacatt cacgcgcttc aacaaatcgt gcacaagacc cgggatgctc
     1021 gacgcaagca aactattccg aagttttctc ctgccgaccg cgaccagctg attcacaagc
     1081 atcatcccga ctaccgcgcc agcgcctatc gacccattcg attcggtccc aatgccgacg
     1141 agaaaaccgt cgtggaattg gcggcactgc tcgaaggcga cagcccgatt ggcgacgaca
     1201 ttgatttgac gcctcactat acgactgatg tgttggtcgt cggaggcggt ggagcgggtt
     1261 gtgcggcggc attgcacgcg caccatgccg gctcgaaagt catcctggcc accaaattgc
     1321 ggctggggga ttccaacagc gtcatggcgc agggggggat gcagatctcg gtcgcgcccg
     1381 aagattctcc gatcacccat ttcatcgaca cgctcaaggg cggccacatg cagaacgacc
     1441 atgacctgct caaggtgatg gtcgaagagg gcccttcgat tgcaaagtgg ttgatcgagt
     1501 tgggcgtgct gttcgatcgg caggccgacg ggaatctcca tgtcaagaaa ggcggcggca
     1561 gttcca
//
LOCUS       DATIFR010000169         1776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1258425, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000169 DATIFR010000000
VERSION     DATIFR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258425"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1439)
                     /locus_tag="VLD60_09825"
     CDS             complement(234..1439)
                     /locus_tag="VLD60_09825"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HSB45300.1"
                     /translation="MSDEKSMGQVIQIDEARIRDHLGEMVRGTVEETLNAMLDAEADQ
                     LCGAGRYERSKARQDTRAGSYERTLHTKAGDVSLKVPKLRRQTFETAIIERYRRRESS
                     VEEALIEMYLAGVSVRRVEDITEALWGTRVSPSTVSNLNKKIYAKIEAWRNRRIEGEH
                     PYLYLDGIVMKRSWAGEIRNVSLLVASAVNSEGFREILGICEGAKEDKSGWSAFLRHL
                     VDRGLKGVQLIISDACRGLLESVAEYLPEASWQRCMVHFYRNVFSHVPATKVREVSHM
                     LKAIHAQESRDAAEKKAKEIIGELRAGKMSKAADLVEQAVPETLTYYAFPDIHWQKIR
                     TNNPLERIMREIRRRTRVVGAFPDGQSCLNLAAARLRYIAGTAWSAKRYMNMRPLHER
                     QFSETGAVA"
BASE COUNT          368 a          550 c          465 g          393 t
ORIGIN      
        1 acctcgtaga tcgcgccgca tatgtgcatg tgaccctgag gtctgccatg gcgagcgcat
       61 gatagcccac cgtgcgtaaa ggcagcagca accgtggcga cggtgtgtgg cgtatgcgta
      121 cccgcgtgac gtttgacgac gtcgctaatc gtcagatttt atgaaaaacc agcgacaact
      181 ttaatcaata caataggtgg ccgggtagcg tctagattct ttcgcacatt tgatcaggcg
      241 acggctccgg tttcagagaa ctgccgctcg tggagtggcc gcatgttcat atagcgcttc
      301 gctgaccatg ccgttcctgc gatgtagcgc aaccgagctg cagccagatt gagacaggac
      361 tgaccatccg gaaatgcgcc aacaactctt gtccgtcgcc ggatctctct catgatccgc
      421 tccaatggat tgttagtgcg aatcttctgc cagtgaatat cgggaaaggc atagtaggtc
      481 agggtctcgg ggacagcctg ctcgacgaga tcagcagcct tgctcatctt gccggcccgt
      541 agctcgccga tgatttcttt tgccttcttc tccgctgcgt cccggctttc ctgcgcatga
      601 atggctttga gcatatggct gacttcgcgg acctttgtcg ccggcacatg gctgaaaaca
      661 ttgcggtaaa aatgaaccat gcaccgttgc cagcttgcct cgggcagata ctccgcgacg
      721 ctctcaagga ggccgcggca ggcatcggaa atgatcagct gaacgccttt gagaccgcgg
      781 tcgacgagat gacgcagaaa cgccgaccag ccggatttgt cttccttggc gccctcacag
      841 atgccgagaa tctcccgaaa tccttccgaa ttgacagcac tggccaccag cagagagaca
      901 ttgcggatct cgcccgccca gctgcgcttc atcacgatcc catccaaata caggtacgga
      961 tgctcgccct cgatgcgccg gttccgccag gcttcgatct tggcgtagat cttcttgttg
     1021 aggttcgaca cggtgctcgg gctcacccgt gtgccccaga gagcttccgt aatgtcctcc
     1081 acacgtcgaa cagaaacgcc ggccagatac atctcgatca gcgcttcctc gactgagctc
     1141 tcccgccggc gatagcgctc aataatggcc gtctcaaacg tctgacgccg cagtttcgga
     1201 accttgaggc tgacgtcgcc cgccttcgtg tgcagcgtcc gttcatagct gcccgcacgg
     1261 gtatcctgtc gagctttgct acgctcatag cgccctgcgc cgcatagctg gtcagcttcg
     1321 gcatccagca tcgcgttcag cgtctcttcg accgtcccgc gcaccatctc cccgagatgg
     1381 tcccggatcc gagcctcatc aatctggatg acctggccca tgctcttctc gtcactcatc
     1441 ggttgctcct tcattctaga agaagccgtc aatcctgaaa gtgcgaaaga ttctctacgt
     1501 tatcagatgg cgtgtccgct tcacccccca atagcgaccg cgaaagcgga ttcccgcaaa
     1561 cggtcatgtg cgcattacct ccaaaagcgg acgtgtgcgg cgcaaaccgt catgtctgct
     1621 ttgggctaat agcgaacgct cgcgtcccat tttcgacaaa ctcaaagtcc gaaggtggcc
     1681 aatcgctcaa gaaaccaaaa tgtcgctgtc gcgccgacca catatgtcgc ggcaggccga
     1741 gcgaattgta tagccatcct cggaatccgg agcatc
//
LOCUS       DATIFR010000170         5074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_876167, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000170 DATIFR010000000
VERSION     DATIFR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876167"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VLD60_09830"
     CDS             complement(<1..1239)
                     /locus_tag="VLD60_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSB45301.1"
                     /translation="MRKSDVFEGSVAIAETARDKASYTGFLAGLFETDVRWDLFTSIG
                     IPQTSPSALEFLERFKAALLPLDPDRVDSEGELPESLLKDFAQLGAFGIKIPRRFDGQ
                     EFTQYEYQKVATLCGSFDASLTVLLSAAQSIGVPEPLRLFGTEEQKAKYLPRLARGEI
                     SGFALTERNVGCDISKVETYAVRVTENGKTVAYRLTGEKFFITNSAKQDGEFLSSMLV
                     VIARIVDRPEELQNPKAQKRYGAFIVETQWPGCSVTRLRFEGVRAIYNGIPTFNNVYV
                     PIENRLGSEEDGLRIALATLTVGRLTLPAACLGGLKQCLSVMRWWGRTRVQWNKPIGE
                     HNLIGEKLCRAAASTLALEAVMAFCGAWANKKGDLRLESAAAKIIGSEWYWEAVNDLF
                     QVRGGRGYMTVEAQRKSGEPA"
     gene            1469..2794
                     /locus_tag="VLD60_09835"
     CDS             1469..2794
                     /locus_tag="VLD60_09835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSB45302.1"
                     /translation="MRNVVVVDGFRTPLCKEGTDFRETDAEVLGAWVVRELITRCHRW
                     NLPLNTIDCVLGSNVATPTHAVNPTRVAAVTGGLPATIPADTVAGKNCGSGVTALYYA
                     SLRIRSGDADTVLTIGMEAMSRISIEYNRTVASHVLQYGKARSFGERTASALALLPKL
                     LNLKRYPPRVGLVMGLTDPMCDLIMGLTAENIAKDPSLGITRADQDAFSVRSHRSAAQ
                     AWKNGLFADEVVPMYVSERGTYVERDNGIREDASLETFRDVKPVFDRYHGSVTPANAS
                     QITDAAAAMLLMEESKAKSLGLPILGYVKDYADIGYEPACMGLAPVGAIAKVLSKAGL
                     TLKDCAVHEINEAFASVVLGICRILDSSSLMERKFGHYGWTKRLGEIPTDDLNPHGGA
                     IALGHPVGVSGLRLAMTSLFELKRRNAERALISACVGGGQGVAMILERK"
     gene            2797..4983
                     /locus_tag="VLD60_09840"
     CDS             2797..4983
                     /locus_tag="VLD60_09840"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSB45303.1"
                     /translation="MFRDSITFPSGYGESEHFKTAVLDGGILLAGFDYVGKPVNVLNV
                     ESLSQWQEIVQFAEQSSTVKGVVLMSLKEGNFCAGADLKQMHDAQQRRSFQEIEQLVT
                     TAHELFVAMGRSPKPFVAAVEGACLGGGLELALACHVRVSSSHPKTCFALPEVKLGIL
                     PGFGGTQRLPRLVGLPAALDMITTGRMVFPRQALRMGLIEGMVTSIPSRLRTLEDIQR
                     EALVQAAIAQARALCSTPFRRRPLRASQRFLSLPGLRSLLCLYARRQVIKRVRHFYPA
                     PLRAIDAVERGMRLSIQEGSLTVEKPLLLDLMASTISAHLVGLFLAGEEVKRKVASVP
                     EAAMRIGVLGAGLMGSQIAGQLAEKGYSVVLRDVASDILATAMGKIHKTQTTQVRKKI
                     ILPSDLRYRVMRIAPTTRMRDVSAASLVIEAVSEKLDVKRAVLAEFESEARPDAIFAT
                     NTSSYTLADIAVKAVHHERCVGLHFFNPVAKMQLVEVVRAPFTSDRALAWASSLAKQL
                     GKFPLVVSDGPGFLVNRILSRYLAEAVIMVGEGIPIRRIDHVAKSFGMAVDSGHPMGP
                     LELLDLIGLPVAVHVLTSLAVLGSRIESRDTLLRNFLPEKKSSITFWKAGKENVQALD
                     AIANYRRANPSDAGPVSDDILHQRLFLPMVDEAVRCLQDKIVEHPWEVDFALTYGIGF
                     PAFRGGLLTWARETMTPGDVTRQLESLAMKYGKRFEPCPGLSNGEW"
BASE COUNT         1029 a         1403 c         1501 g         1141 t
ORIGIN      
        1 ggcaggttcg ccgctcttac gctgtgcctc aacggtcatg tatccgcgtc ccccgcgtac
       61 ttgaaagagg tcgttgaccg cctcccagta ccactcgctt ccgataattt tggctgccgc
      121 cgactccaac cgaagatccc ctttcttgtt cgcccaagct ccgcagaagg ccatcactgc
      181 ctcaagcgcc aacgtagagg ctgcggcccg gcagagtttt tctccgatca gattatgctc
      241 gccgatgggt ttgttccact gcacgcgggt ccgcccccac cagcgcatca cggagaggca
      301 ctgtttaagg ccccccaaac aggcggcggg aagcgttagg cggcccacgg tgagtgtggc
      361 gagggcgatt ctcagaccgt cctcttcact gcccaggcga ttttcaatcg ggacgtatac
      421 gttgttgaac gtcgggatgc cgttataaat cgctctgacg ccttcgaacc gcaggcgagt
      481 gacggaacac ccgggccatt gtgtttcaac gatgaaggcg ccatatcgtt tctgcgcttt
      541 ggggttttgg agttcctctg gacgatccac gattcttgcg atgacgacca gcatggaaga
      601 caaaaactcg ccatcctgct tggcagaatt cgtaataaag aacttttctc ctgtgaggcg
      661 ataggcgacg gtctttccgt tctctgtgac gcggaccgcg taagtttcta cttttgagat
      721 atcgcagccc acgttgcgtt ccgtgagcgc aaaccctgaa atttctcccc tcgcgagccg
      781 gggaagatac ttggctttct gctcttctgt tccgaagagg cgtagcggct cggggacgcc
      841 gatcgattgc gctgccgaca acagcaccgt cagcgaggcg tcaaagcttc cgcatagggt
      901 ggcgactttc tgatactcgt attgggtaaa ctcctggccg tcgaagcgcc ttgggatctt
      961 gatgccgaac gcgccgagtt gggcgaagtc ttttaacagc gactcgggca gttcaccttc
     1021 gctgtctact cggtcgggat ccagggggag aagggcggcc ttgaatcgtt caagaaattc
     1081 cagggcggac ggactcgttt gagggatgcc gatcgaggta aagaggtccc atcggacatc
     1141 agtttcgaag aggcctgcaa gaaaaccggt ataggaagcc ttgtcgcggg cggtttcagc
     1201 gatcgccaca gagccttcaa atacatccga ctttctcata cgcagcgttc ctctggcgac
     1261 catggctgga gctgcctaat cctggctagc ttaccaccat tcacaccctc attgtctgat
     1321 gctcagaagg attactttcc ccctcgaatg cgggattggt ccagggttcc gaaaagcgag
     1381 gcgaagcacc atttccatgg taccatgttc atagatagag catcggcgag tgatagttct
     1441 tctccggata tgagacggag attgtacgat gcgcaatgtc gttgtcgtcg acggatttcg
     1501 tactcccctc tgcaaggaag gaacggactt tcgcgaaacg gatgcggaag tgcttggcgc
     1561 gtgggtcgtc cgcgaattga tcacgcgttg tcatcgttgg aatctccctc tcaatacgat
     1621 cgattgtgtg ttgggaagca atgtggcgac gccgacgcac gcggtgaatc cgacgcgtgt
     1681 cgcagccgtc accggtggcc tccctgcgac gattcctgcc gatacggtcg cggggaaaaa
     1741 ttgcggctcc ggagtaacgg cactctacta tgcgagcctg cgcatcagaa gtggtgatgc
     1801 cgacacggta ctcacaatcg ggatggaggc catgagccga atctcgatcg aatacaaccg
     1861 cacggttgca agccatgttc tgcaatacgg caaagccaga agctttgggg aacgcactgc
     1921 cagtgctctt gccctccttc ccaagctcct caatttgaag cgctatccgc cacgggtggg
     1981 gttagtgatg ggtctcacgg atccgatgtg cgatctcatc atggggctta cggcagaaaa
     2041 tattgcaaaa gatccgtctc ttggtattac gcgcgctgac caagacgcgt tttctgttcg
     2101 gtcgcatagg agtgccgcgc aggcatggaa gaacggactg ttcgcggacg aggtcgtgcc
     2161 tatgtacgtt tccgagcgag gcacctatgt cgagcgggac aatggaattc gtgaggatgc
     2221 cagtctagag acgtttcgtg acgtcaaacc tgtcttcgac aggtaccacg gctcggtgac
     2281 accggcgaac gcctcgcaaa ttacggacgc agccgctgcc atgctgctca tggaggagag
     2341 caaggccaag tctctcggct tgccgattct cggatatgtg aaagactacg ccgatattgg
     2401 gtatgagccg gcttgcatgg gcttggctcc cgtcggggcc attgcgaagg ttttgtctaa
     2461 ggctggcctg accctgaagg attgcgcggt acatgagatc aacgaggcct ttgcatcagt
     2521 cgtactggga atctgccgga ttctggactc gtcatccctg atggaacgaa agtttgggca
     2581 ttacggatgg acgaagcgtc ttggcgaaat cccgaccgac gatctcaacc cgcatggcgg
     2641 ggcaatcgca ctcggtcatc ccgttggcgt ctccggtctg cgcttggcca tgacatccct
     2701 gttcgagctc aaaagacgga atgccgagcg ggcactgatc agcgcctgcg tcggaggggg
     2761 acagggtgtg gcgatgatct tggagaggaa atagccatgt tcagggactc catcaccttt
     2821 cccagcggtt acggcgagtc cgagcacttc aaaactgcag ttcttgacgg agggatcctg
     2881 cttgcaggtt ttgattacgt cggcaaaccg gtcaatgtgc tcaatgtaga gagcctgtcg
     2941 caatggcagg agatcgtaca gtttgccgag caatcttcca ccgtcaaggg cgtcgtgctg
     3001 atgagcttga aggaaggaaa tttctgcgcg ggagccgatc tgaagcagat gcacgacgcg
     3061 caacaacgac gctcctttca ggagatcgag caactcgtga ccacggccca tgagctcttt
     3121 gttgcgatgg gcagatcgcc aaaacctttt gtcgccgcag tggaaggcgc ctgtctgggt
     3181 ggtggacttg agttggcgct ggcctgtcat gtacgcgttt ccagtagcca tccgaaaacc
     3241 tgcttcgccc tgccggaagt caaactgggc attttgcccg ggttcggcgg cactcaacgg
     3301 ctaccacggc tggtcggcct accggcggca ttggacatga tcaccacggg gaggatggtt
     3361 tttccccgac aggctctgcg aatggggctc atcgaaggca tggtgacctc tattccgtcc
     3421 cgtctcagga cgttggaaga cattcaacgt gaagccctgg ttcaagctgc tattgcgcaa
     3481 gcccgcgccc tctgttcaac tccgttccga cgacggcctc tccgggcaag ccagcggttc
     3541 ctgagcctgc ccggtcttcg ttcgctgctg tgtctctacg cgcggcgaca ggtcatcaag
     3601 cgcgttcggc atttctatcc ggctccgctc cgtgccatcg acgcagtgga gcgagggatg
     3661 cgtttgagca ttcaggaagg ttcgctcacg gtcgaaaagc ctctccttct cgatctcatg
     3721 gccagcacga tttccgcaca cctcgttggt ttgttcctgg ccggtgagga agtgaaacgg
     3781 aaggttgcct ctgtcccaga ggcggcgatg cgtatcggtg tactgggagc cggattgatg
     3841 ggttcacaaa tcgcggggca gctcgccgaa aaaggctatt cggtggtgtt gcgagatgtg
     3901 gcttcggaca ttcttgcgac ggcgatggga aagattcata aaactcaaac gacccaggtc
     3961 cggaaaaaga tcattcttcc ctccgatctg cgctatcggg tcatgcggat tgctccgacc
     4021 acgcgcatga gggatgtgag cgccgcgtcc cttgtcatcg aagccgtgtc ggagaaactg
     4081 gatgtgaaac gtgcggtgct tgcggagttt gaatcggagg cacgccccga tgcgatattc
     4141 gccacgaata cctcgtcata taccttggcc gacattgccg tgaaggctgt ccatcatgag
     4201 cggtgcgtgg gactccattt cttcaaccct gtcgccaaaa tgcagttggt cgaagtggtg
     4261 cgcgcaccct tcacatccga cagggccctc gcatgggcct ctagcctcgc caaacagctt
     4321 ggcaagttcc cactcgtggt gagtgacgga ccgggattcc tcgtgaacag aattctgtca
     4381 cgttatctgg cagaggccgt gatcatggtg ggtgagggca tcccgatccg gcgcattgat
     4441 catgtggcta agagtttcgg tatggcagtg gacagcgggc atcccatggg tccgctggaa
     4501 ctgctcgatc tgatcgggct tcccgtcgcc gtgcacgtgc tgacctcgct cgccgtcctg
     4561 ggatctcgga tcgagtcgcg tgatacgctg ctgaggaact tccttccgga gaagaagtcg
     4621 tcgatcacat tttggaaagc aggaaaagag aatgtgcagg cgctcgacgc gattgcgaac
     4681 tatcgtcgcg caaacccttc ggatgccggc cccgtctccg acgacatcct tcatcagcgc
     4741 ctgtttcttc caatggtcga cgaagctgtg cggtgcttgc aggacaagat cgtggagcat
     4801 ccctgggaag tggattttgc cctgacgtat ggaatcgggt ttcccgcgtt tcgtggggga
     4861 cttctcacct gggctcggga aaccatgacg ccaggagacg taacccggca attagaatcg
     4921 ttggccatga agtatggcaa acgcttcgag ccctgcccgg gtctctcgaa cggagagtgg
     4981 tagtggcaag actttcttgg gccaaccaaa acggagcgct tcatgcctga tcaaccgtgg
     5041 tttctccatt atcctgaggg agtgccccat gagc
//
LOCUS       DATIFR010000171         1372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_876931, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000171 DATIFR010000000
VERSION     DATIFR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876931"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..>1372)
                     /locus_tag="VLD60_09845"
     CDS             complement(171..>1372)
                     /locus_tag="VLD60_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB45304.1"
                     /translation="ERKRSVIGRAARSIRQLTAMFDVLVTGDGEVAVFEALQDSPPKI
                     VDGDDPKSQLFLNNAALEEMPFPARHLVDVSTYQYSIDGTRALSMIAQLGCPFGCGFC
                     GGRMSPFLRRVRTRSSENIVKEMVHLHHTYGVSGFMLYDDELNVNPKLVSLMSLIANT
                     QQQLGVEFRLRGFIKAELFTDEQAAAMYQAGFRWILVGFESGHPRILDNIQKKATRDD
                     NTRCMEIARRHNLKVKALVSLGHPGESEETIRATRDWLIDVRPDDFDATVITTYPGTP
                     YFDEAVETMPGIWSYTCPGNQDRLHSVEVDYRHVADYYKGVPGNYTAFVYTDELTSER
                     LVGLRDWLEADVRRTLAIPFNDANPGLRYEHSMGQSGLPPSILRTSAPMSARVTHKTI
                     RLLSSGG"
BASE COUNT          310 a          380 c          385 g          297 t
ORIGIN      
        1 acaagcaagg ggagtcggga cggatcaatg aaggcagtgc cgagaatgac aacgagggct
       61 gcgaggacga aggttgaaac agtcgtcgta tacggatctt cttttgacat ggcgtcgatc
      121 acaatagcgc gaacactttc agaaaccgag accaagcgtt ctgattccga ctatcctccg
      181 gaggacaaca gccggatggt cttatgagtc acgcgtgcgg acatcggggc cgaggtccgg
      241 aggatcgatg gagggagtcc gctctgcccc atggagtgtt cgtagcgaag gcctgggttg
      301 gcatcgttga acggaatcgc gagcgtccgg cgcacatcag cctccaacca gtcacgaagg
      361 ccgacgagtc gttcgcttgt caactcgtcg gtgtaaacaa acgccgtata gtttcctgga
      421 acgcctttgt aatagtcggc gacatggcgg tagtccactt ccacgctgtg cagccgatcc
      481 tggtttcccg gacacgtata gctccagatt cccggcatcg tctccacggc ttcatcgaaa
      541 tagggcgttc ccgggtaggt cgtaatcact gtcgcatcga aatcgtccgg acggacgtcg
      601 ataagccagt cacgggtagc gcgtatcgtc tcctctgatt ctcccggatg ccccagtgac
      661 acgagggcct tcaccttcaa gttgtgccgc cgcgcaatct ccatgcagcg tgtattgtcg
      721 tcccgggtcg ccttcttctg aatgttatca agtattcgcg gatggcccga ctcgaagccc
      781 acgaggatcc agcgaaagcc ggcctgatac atcgcggcag cctgttcgtc ggtaaacagc
      841 tcggccttga taaagccacg caaacggaat tcgaccccga gttgctgttg agtattagca
      901 atcaggctca tcaacgaaac gagcttgggg ttcacattca attcgtcgtc atacagcatg
      961 aaccctgaga cgccgtacgt atgatggaga tgaaccatct ccttgacgat attctcagat
     1021 gagcgggtac ggacccggcg tagaaacggg ctcatcctgc ccccgcagaa tccacagccg
     1081 aaaggacagc caagttgtgc aatcatggag agagctctcg tcccatcgat gctgtactga
     1141 taggttgata catcaaccag atgacgtgcc ggaaacggca tttcctctag cgccgcattg
     1201 ttgaggaata actgtgactt cggatcgtcg ccatccacga tttttggagg tgaatcctgg
     1261 agcgcttcga agacagccac ttcgccgtct ccggtgacga ggacatcaaa catggcggtc
     1321 agttgcctga ttgatcgggc tgctcgtccg atgacgctcc gcttgcgttc at
//
LOCUS       DATIFR010000172        28478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2028756, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000172 DATIFR010000000
VERSION     DATIFR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028756"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VLD60_09850"
     CDS             <1..1149
                     /locus_tag="VLD60_09850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HSB45305.1"
                     /translation="VESLRGLAALMVAICHSQLVFVVDGHEDLWCLNIADVQGLQALA
                     TKVVLALFNGGAAVSLFFVISGVVLGLSLDRQKDEIVRSSVSFLIRRALRIYPALILS
                     LLLVQAWLPLIYPAPDYVGASLMFQPLYRVPQAADFFANLFFVSNQMNPVIWTLKIEM
                     EVALLLPILHLINRSIPPSGNMAVLALLMWLSTNGHDTSTEKWVFAFYLGLMLPEWGP
                     WLVSAMRTSPAGLEMCLGSSLVAFGAARHLLFGTGYAVITPFVEGICAMTLLACVVYQ
                     PALKWWLVLDWRGIRALGRLSYSFYLFHFIVHYLVGVGLFRLVSTQALSTYPIVWHGL
                     LGIVSVALTVPLAVVSYRWVEKVFIELGKDLTRRIEVSFRRPMPEGVR"
     gene            1270..2268
                     /locus_tag="VLD60_09855"
     CDS             1270..2268
                     /locus_tag="VLD60_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSB45306.1"
                     /translation="MRLVRLFVLVVMIGGWAVTVQSKDLSPREIYEQASPAVVLVMGH
                     SEGGRTGSGGTGSIIQADGLVLTNAHVVIEERTGKPYPRLSVFLKPARVTGDSKVDLS
                     RMMRARVVAFSTPLDLALLKLDGAPSALPVVAFSESSQERIGDRVVAIGHPEQGGLWT
                     LTTGVISAEVDNFNGVPGKHVFQTETGLNRGNSGGPLLDGEGHMVGVNTAIVRVAPDG
                     LPITSISFSLKSNVAAQWLREQGVGVKAARGVTVPTGQTSPMTDVPAAPSVPLPGRAV
                     PVPKQVAPSQPPAKSDRLDSTPHRPYDLDQLVSDRAKAEADLEGMMSEMRGRMKGK"
     gene            2294..3130
                     /locus_tag="VLD60_09860"
     CDS             2294..3130
                     /locus_tag="VLD60_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45307.1"
                     /translation="MFMNPRTSRPLFVVVALCWLVVASPATSSAKSDLDRFELVGFVR
                     AVVTKHPQLKTTQQFDHDGNLTSLELVPAKEANSARYVYLHDASGRITEEHTVDPDGT
                     VTNRKQFRYGVDEQGRQLALVAVTDEGLFAHAEFSVYDGRGLLAEQIMVSGQGIAEKS
                     LYDVRGNLIYDARYFQGRLVLEATHHYGPLDRLTESRFYGSDGELMRKDLYRYDQAGN
                     RVEQTSEFYHQSHLRKSVVAYEFDHMGNWTKETIQRWSDKNGAPTLGETVVSRERTIT
                     YY"
     gene            complement(3207..4769)
                     /locus_tag="VLD60_09865"
     CDS             complement(3207..4769)
                     /locus_tag="VLD60_09865"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HSB45308.1"
                     /translation="MQGTGRTSRPQRENIVGVIVFVAALTLYISTLAPSYLWGDPTKL
                     LFYVLDHHFIGLGPGYGTHPLHNVLGYIFSFLPFSFAYSQNLLSAWFAAAAVYLVFRI
                     IMETFEDLFAALAAAASMAVSHVFWLYAVLNESYSLLSFFTLLAFLLCLKWIKQPRDW
                     HLYVLAATVGAGLANHALLVLAFPGFLALLWGKALFEFMFSWRIAVVFLAFLAGSCQI
                     FLIPIMASGSLTAFAAQLVGDTSNTYQTFSGTFLQLIKELGGSPFYLLYQFPGFAIVL
                     GALGLVIGFQRSPRLILSTVLIALPILLFTSQYMKQRQFPMLIVTFSMFALWVGAGTS
                     YLLDKFPRLRSTPICSALLASLVLCPPTVYYSASRIAEAVQLDVSFIRTLPYRNSYSY
                     YLFPPKNRERGPQEYAADAFRQAKTGALILADFNPGLVLVYEQRVMGQRKDLEIGIYI
                     DDWSHYSSDPTAATMDFLREQVVAKGRILYLADDWDPYYHSAEIRKEFHLERVGGPLW
                     EVTAKSKPSPSN"
     gene            complement(4788..6461)
                     /gene="ilvD"
                     /locus_tag="VLD60_09870"
     CDS             complement(4788..6461)
                     /gene="ilvD"
                     /locus_tag="VLD60_09870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSB45309.1"
                     /translation="MPKELKLKSHDLLVGPGRAPARAMLKAVGFTDEDLSRPIIGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDGVVALSGCDKTIPGTVMALARLNLPSLMLYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHASGRMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGISPM
                     GLNGVPAMDNRKDDVAFECGKMVMELLKNDLRPRQIITRKSLENAIAAVATTGGSTNA
                     VLHLLAIAREADIKLTIEDFDKINRKVPLLADLKPGGRFAAADLYAAGGTTLVAKRLL
                     DAGLLHQNQPTVTGRTIGEEAQRATETPGQQVLRPLGNPIKKTGGLVILKGNLAPEGC
                     VVKVAGHSMMHFSGPAKVYDREEDAFVAVKAGQIKAGDVVVIRYEGPSGGPGMREMLG
                     VTAAIVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAVRGGPIAAVKNGDTIVFDIA
                     KRQLNVSLSRKEIMARLKKVKQPLPRYTSGVMAKYARHVSSASEGAVTT"
     gene            complement(6469..6825)
                     /locus_tag="VLD60_09875"
     CDS             complement(6469..6825)
                     /locus_tag="VLD60_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSB45310.1"
                     /translation="MKLFNLVLTLAIGTVLAVASPAWSDKKKDGEDDVAALVKDAKVT
                     IDQAVKTALEKVPGTAVEAELEKKHDKTVWEVEVVGADGKVSEVHIDAATGAVIDVEA
                     KKHEKHEEKKEGKKGK"
     gene            complement(6856..7500)
                     /locus_tag="VLD60_09880"
     CDS             complement(6856..7500)
                     /locus_tag="VLD60_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45311.1"
                     /translation="MTKATKTMSTLGIVSAFALMLGLPAIWANEPGYGKDGHSGGGHS
                     AMGGHGMGMMHSSAGHLIRHLLKHEKDIGLTADQVAKLKNIQLNLDKARIKAEADIQI
                     AERELKALTDDEKSDLGAIEAKLKQSEDMQVALRMTSIKTRREVLGLLTPEQRAKEKA
                     EHDKMMQEHKEGGKAPAGPHGGGMMNPHGVSPHGTPQKGSDGSVPAPPSSMSVN"
     gene            complement(7547..8182)
                     /locus_tag="VLD60_09885"
     CDS             complement(7547..8182)
                     /locus_tag="VLD60_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HSB45312.1"
                     /translation="MTQTEYAVLAFSSLFVIVDPIATVPAFLAMTARDTLNQRLRMAR
                     TACVVMMGILMGFALVGQSLFSLLGITLPAVQVAGALVLLLVSLDMLRAQRSPVQETA
                     AETAEGATKDDIAITPLAVPMLAGPAAISTVILLDAQAASWTYRGVLLACVALVALAS
                     YAILAVGASGAKWMSSLAEKIITRLMGLLLAALAVQFLFNGLKGEGGLFVR"
     gene            complement(8188..9216)
                     /locus_tag="VLD60_09890"
     CDS             complement(8188..9216)
                     /locus_tag="VLD60_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSB45313.1"
                     /translation="MATTARDYYQILGLSRTATADDIKKAYRRLARQYHPDLHSGSKK
                     AEMEKKFKELNEAHEVLSDPDKRKKYDQYGAQWEQAEAYEKARQQAGAQGFGGGQAGG
                     SFGGEAFSDIFENLFGGRGKSGGGRGFAVPGDDLETEVDLTLREVLTGVTKRVSLREP
                     LPCSTCQGTGAFRNRTCPTCFGSGSTTEAKTIEVKIPAGVQDGTRVRVAGKGQPGHNG
                     GKRGDLYLHVVIPPDPVFRRQGSDIHVMLPVYPWEAALGAEVMAPTLTEPVRVKVPAG
                     SRAEGKLRLKGKGLPSAIGGHGDLFLTLQIVMPPSLTEEEQTLYERLGRGKHPDPRTE
                     LLNQTRMR"
     gene            complement(9310..10248)
                     /locus_tag="VLD60_09895"
     CDS             complement(9310..10248)
                     /locus_tag="VLD60_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45314.1"
                     /translation="MNCTKCKTKAVIKLPRHHAAFCKGCFNGFVHDQVAKAIKSQKMF
                     GKDQRILVAVSGGKDSLALWDILLKLGYKADALYVNLGIGGYSDRSYDKVRLFADSVA
                     APLGATLHTHKVEQEEGSGIRELAQLVHRPTCSTCGTIKRYQFNRAAIEHEYDVMATG
                     HNLDDEAARLLGNVLHWQEEYLDKQGPSLPASVEGFAKKVKPLYRLTERELAAYCVLN
                     MIDYIVDECPMAQGARSLLYKEVLNRLETESPGTKQTFYWGFLEKQSKKESAPTTMAE
                     KDQALLHPCRICKQPTTSEVCSYCKLMARAKTPTTL"
     gene            complement(10330..10524)
                     /locus_tag="VLD60_09900"
     CDS             complement(10330..10524)
                     /locus_tag="VLD60_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSB45315.1"
                     /translation="MQVQLSHPARTVEIKGPKRAKDVLRELSLVVEAHLVIRGDELVT
                     EDEMLFDKDQIEIRPVISGG"
     gene            complement(10638..11159)
                     /locus_tag="VLD60_09905"
     CDS             complement(10638..11159)
                     /locus_tag="VLD60_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSB45316.1"
                     /translation="MFRILKKSLKTGVVTGQHPQAKQPAEQLAPEVRGKASPFRRSLT
                     IREVDTGSCNACEMEMNALANPVYDLERFGMHIAASPRHADALVVTGPVTVNMEHALK
                     DVHKQTPDPKIVIALGDCAIHCGVFKGSYAVTGPVDRHIPVDVRIPGCPPRPAEILKA
                     LENLREQRQSSEK"
     gene            complement(11260..12852)
                     /locus_tag="VLD60_09910"
     CDS             complement(11260..12852)
                     /locus_tag="VLD60_09910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSB45317.1"
                     /translation="MVDAITGVKQLKEAYGNSIEEMPPVHGTPRLRIKKEDVPVVAHY
                     LHIHPGLRGVLSLLWAVDHRPHEARYELCYLFTLAERKDWLLLCTDLRGDDRLFASIT
                     PHIHAAKWYEREIRDMFGLIPVGHPDMRRLVRHEHWPKGSHPLKKDFQWDTVLERAQG
                     QYAFRQIEGEGVFEVPVGPIHAGIIEPGHFRFSVAGEPIMQLEVRHFWKHRGVEKLFE
                     QQTLTTAVPLAERVSGDTTVGHSLAYCQAVETLLGIDVPRRARYLRSLFLELERLHNH
                     LGDVGAICNDTAYALPHAHCSRMKERVMQLNDRLTGSRFLRESMTIGGVARDIPAHQR
                     CEIVDLLDQIEKDFSEIESIIFANASLTDRLETTGVLTERTAWDHGVMGVVGRASGID
                     QDVRRDRPFAAYDEFPPHVALYRYGDVRARLRVRLDEIHDSIRLIRQIRNKIPQSPLV
                     AAPVRSPNPGEWALSSVEGWRGEILYFVMAGENGRIHRCKVRDPSFVNWPAIQWAVVG
                     NIIPDFPLINKSFNLSYAGNDL"
     gene            complement(12857..14401)
                     /locus_tag="VLD60_09915"
     CDS             complement(12857..14401)
                     /locus_tag="VLD60_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSB45318.1"
                     /translation="MQTIIAPVAVFLGAPLLAAFFSLAIRHARVLHGLNLATMIGLVV
                     SEIALIQQVLAHGPLTALWGLVYIDALSAFILFIVTVIGLSCSLYTWAYLDDYVAQGV
                     IAPRRLSRFFFLFHVFLFAMIVATMANNLGVLWVAIEGTTLATTFLIAFFRKREGLEA
                     GWKYLILCSVGIALALFGTVLTYYSSVRVLGDESAALNVTKLLEVAGQLDPHVLKLAF
                     IFLFVGYGTKIGLVPMHSWVPEAYSEAPAPVTAMLAGVLETVAVYAVLRSKAIMDHAL
                     PSAFSGNLLLLFGLLSFVVAALFILIQHDYKRLFAYSSVEHMGLAMVGFGVGGPVGTF
                     GGLFHLLNHALAKSLAFFAAGNVHRRFATREIGEVRGLAKTQPVTAVAILIAGLALVG
                     MPPFSMFVSEVLVVSALATQNFASDTLHLGRFLTIVVADDVRSLSIVTVFLLFAVALF
                     GGFTYRLTAMVWGTPQESVKRGEGWDIGHVPLIFTSLALVGLGITLPDPIRTLLDRAV
                     TVLLAR"
     gene            complement(14401..15882)
                     /locus_tag="VLD60_09920"
     CDS             complement(14401..15882)
                     /locus_tag="VLD60_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSB45319.1"
                     /translation="MAWPLLVLVAAPLIGGFGSLLVRQRRAVEVLQCGLAGAMLASAA
                     LVAERVIAAGHVSVGNLLQADALSAWLDLIIGGVGSTGALYAVGYVGEELDRAHLSLR
                     RYRQFFCLFDLYLGAMLLAVNLENIAIMWIAIEGSTLSAALLIGFDRSKAALEAGWKY
                     VILSSVGIALALFGTILVYYSSEHVLGVSSEALRWSELYRVAEGLDPGAIKVAFIFAL
                     IGYGTKAGLAPMHTWLPDAHAEAPTPVSAMLSANMLTLAVYAILRFKAVTDRTVGPEF
                     AGNLIIGLALLSIAISATFFLVQRDYKRLFAYSSIEHIGIALLGFGVGGLGVFAGAWH
                     LMNHAFAKSTAFYGAGLVFLRHNHKLLDRVTGLLGQMPVAGTAILVAGVALAGMPPFG
                     LFTSEVLIAAEAYAARPEVAYGFLALLALAFATLLYQVLRMVLGAPGEAGTALSRHCR
                     FFTSAAVGINIAVLGTIGLRVPPGLEILLASIVKMYRVGMEAP"
     gene            complement(15889..16557)
                     /locus_tag="VLD60_09925"
     CDS             complement(15889..16557)
                     /locus_tag="VLD60_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HSB45320.1"
                     /translation="MPLLTPHIGSQMVDLGSALLLLTCFAIVAQRRLSACVDLFALQS
                     VFLALTAALVAFLTGIHHIYIAAALTVVIKAVVIPRILKQVIERLNVTRELVMNVNVP
                     AGLMICGGLVILAFFITQPIIPLGFLLTRDSLAIALAIILIGFFTMIARKKAVTQVIG
                     FLVMENGLFLGATAAAYGMPLIVELGVFFDVLVGALIIGIYTHRLQDAFDSVDTTKLT
                     ILKE"
     gene            complement(16559..17509)
                     /locus_tag="VLD60_09930"
     CDS             complement(16559..17509)
                     /locus_tag="VLD60_09930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HSB45321.1"
                     /translation="MLQAIVLIVAQTIVLLAVSPFIVGLIRKVKARLQVRRGASVFQP
                     YADLAKLFRKQPVVSTTTSWIFTVTPYILFASTLTAGLLVPVFASRTPLTFAGNIIAL
                     VYLLALGTFFLILAGLDAGSAFGGMGSSREAIVASLTEPAMILSIFAIALTAGSTNLS
                     TIVHKTALLEGIVTDPSPHLMALAALFIVAIAETGRVPVDNPASHLELTMIHEAMILE
                     YSGRYLALVEWAAGLKLLVFLTLIVNVFAPWGIATSLEPAALGLGVVVYLAKVSALAV
                     LIGVIESMFAKLRLFRVTDLLGVAFILALLGLVFFYVLRG"
     gene            complement(17514..19541)
                     /gene="hyfB"
                     /locus_tag="VLD60_09935"
     CDS             complement(17514..19541)
                     /gene="hyfB"
                     /locus_tag="VLD60_09935"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HSB45322.1"
                     /translation="MLNLLWILLGGYAVGMLLPFCLPRQRDAQGLAASISAVVATGAG
                     IVLGFLGLTASEPVVGSIASTIPLLTFAIRLDALAAFFVLTISLAGLAASIYAIGYVR
                     HFPGHVSIAALGSLFNAFLGSMTLVVLADNGFFFLIVWELMSLVSYFLVVTEHEKAEV
                     RYAGFFYLIMTHVGTAFIILTFLILFQADGSFSFEIFRHPEQPLPEGMRTLVFLMALI
                     GFGTKAGIVPLHVWLPYAHPAAPSHVSALMSGVMIKTAIYALIRVYFDFLGGQFPWWW
                     GFVVLVIGAVSALLGVMYALMEHDMKSLLAFHSVENIGIILLGIGAGMIFQTYGLKEL
                     AALGLLAGLYHTINHAMFKALLFLGAGSLLYATHTRDMEEYGGLLRRMPWTGLFFLIG
                     AVSISALPPTNGFVSEWLVFQSLFLSFQMPSLLMKLMLPIAAAMLALTGVLALTCFAK
                     AFGISFLALPRSSHARHAEEVPFSMRLGMALLAIVCIVLGVAPMVAVPLLDRVTAPFV
                     GVSIEDKVLAMDGWVLAPVNVEFSSISTPVLAFLLVALSMLGLGLVATFGGLVKKRYY
                     KTWGCGINLTPRMEYTATGFVQPIKRVFSTIYQPTVKLETEFLEESRYFAKRRRFEFH
                     IEPIFQKYLYDPVVAFFTTMAGRLRVIQAGSLHLYLTYIFVTLVILLLIAV"
     gene            complement(19709..20638)
                     /locus_tag="VLD60_09940"
     CDS             complement(19709..20638)
                     /locus_tag="VLD60_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB45323.1"
                     /translation="MRYFVTALIAIMTGIGSPAWSYQEVAVTDGGTLTGKVTLDGKVP
                     KPKGYNLVTLPDQYYCGRISDGQGWRILQPFNVGPAGEFRDVVVYLEGINKGKPFDEK
                     GVPQIEARDCLFVPFTTVVRDDQSVTVVNMDPVMHDIQAYETSHLGARVLFNVPLPMN
                     PQHPRNLKDRSDAGMYHKHMAGAPMKQLVNLSKNRRIFVMQCGFHAYMESWGVAVTNP
                     YFAKTDEQGRFTITNVPPGTYKMVVWHPYVRSAIEQTVTIGPKGTVEAKIAVPAPTGR
                     LYANEVMDHPYIRYNVSEETQREIDPMIKKQDH"
     gene            complement(20723..21301)
                     /locus_tag="VLD60_09945"
     CDS             complement(20723..21301)
                     /locus_tag="VLD60_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HSB45324.1"
                     /translation="MKPYEMTVGPEGYLPPSVSERGVIGPSKGEGLVMGKRVPEQQAI
                     DETARRLIQAKNPTIFPGPLVLWAWNEQAVRKSKVVKALANAVPARLIPMADYRPKYP
                     KIVAEHEINPNHPNLTIWKNKIDVCLFVGVHCHQSNIALKIIRGGTDCFTIALCTFNG
                     DDEAHVTIRDLTADTIQRIVDSVHRQKRTRPS"
     gene            complement(21332..22777)
                     /locus_tag="VLD60_09950"
     CDS             complement(21332..22777)
                     /locus_tag="VLD60_09950"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45325.1"
                     /translation="MAPLTAWLPADSDQDFDLVVKVVGPGASALLKHWSPIAQCLIDR
                     DFAVMAQSGKFNGDRGDDLLHIRVATRTFYSMGNGCDVLICLSEHLPEFRQFGLQPGS
                     VFLWEPPTHPRVSPVLPEGVVPYHIPLSTLCASCGEGVIGKGFAALGALLQLLGIQEE
                     ALPGLTASAAIPRSFAAGWAYARRVLEKHDAYALPQSQVPDAHSQVLLTPHQAIMLGF
                     AVSACDCGEACSGELNESAEQWVTRHTGMAGAIVSMLESKSQPGLQAYRGPQGKVMAI
                     LQGDTSSIGTCLDAFTAPKVFVAPDISNALGLLLAGHALIRDGLSDGVGVLIEETVAR
                     RQQSVEIRSLVGRIRRGSTDLGNTAAPYQPDPVTAMVDREEGTKADVGFVAWGAAQGV
                     VRDAVTLCRNFGLNVASLYPKVIVPFPKEELESFARTVKRIVLVESNRTHGYKERLRA
                     ACSFPASVLTPPPGQALTPMDIFLRESLGAV"
     gene            complement(22795..23073)
                     /locus_tag="VLD60_09955"
     CDS             complement(22795..23073)
                     /locus_tag="VLD60_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45326.1"
                     /translation="MSEHADIVVIHYPRGATALVWCDLTTGAIATSHAGLQALLRRGL
                     RDWEGHPVCPHDGHVFLSAVYDHFFLSGYSVRWLSVSGLKELRNAYRV"
     gene            complement(23360..23632)
                     /locus_tag="VLD60_09960"
     CDS             complement(23360..23632)
                     /locus_tag="VLD60_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45327.1"
                     /translation="MPTIWGCLSRIEDDLHGTPWVEVKAILSVRTTIRVRALVGRQTL
                     IARGIERVDLSSLCAGELVEVSYNRGRDGLMQAETIYVRPDRVAVA"
     gene            complement(23988..24668)
                     /locus_tag="VLD60_09965"
     CDS             complement(23988..24668)
                     /locus_tag="VLD60_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HSB45328.1"
                     /translation="MGSAKLERIIDVGPAGFHPPSAMEMGVTHPDSGFGLLYGHHAPE
                     DVVIAEAARQLYTRKNPTIFPGPLYCWAWHHEWIAKGQALLKLAAEIPDVMIIPMPDY
                     RPKYPKIDPEEGINPNHPNLTIWGNKIEATLFIGIHCHYANLALRMVRAGTNCLTVAF
                     CHDIHEDAMLSVQDLDVKKMDHIISIFRTVRKELGIGLPKDGKTVRLTGTQIRANHGV
                     ERVHPSRA"
     gene            complement(25105..26463)
                     /locus_tag="VLD60_09970"
     CDS             complement(25105..26463)
                     /locus_tag="VLD60_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB45329.1"
                     /translation="MTTPAHILVVEDEVNIRSALITMLEKLGHTVSGAGTAEEALALL
                     EDSSADLVITDLRMPGLSGMEFLRRMKERWPETEAIVMTAFGSIDTAVEAMRRGAYDY
                     LTKPIDRERFPVVVAKALERHVLATENRQLRDRLETRTRFDQMVGESAPMQRVYSLVE
                     MVAESDVTVLLTGESGTGKELVARAIHHKSSRANGPFVTLNCGALPENLFESELFGYE
                     KGAFTGAMNNKMGRFELADGGTLLLDEVGELSLKSQVDFLRVLETKEFRRLGGTKLIK
                     VDTRIIAATNRNLEEAVKRGDFREDLYYRLNVVPIRLPPLRERGDDIPLLLDRFLDEF
                     SAQHHRQPKEVSRDAMRLLRLYAWPGNIRQLRNLIERIVVTMKDPVIQPDHLPEEIQA
                     SKEDARTMVVTLGSSLEDIEREAIRRTLAEVTNHREKAAKLLGISLRALQYKIKEYGI
                     RE"
     gene            complement(26460..27854)
                     /locus_tag="VLD60_09975"
     CDS             complement(26460..27854)
                     /locus_tag="VLD60_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45330.1"
                     /translation="MFRPSPETTLFQIIPLRLIATVLLILALMVSALLLFSFEHEQFV
                     VQGLTEGRDVPTELLGGDWQSRYDLVVMTILLFLLSGIGIAAVITFQHYNSTRRTLEE
                     VKGLARNILESIPTGVVTVNRDGQITAVNPSAVATLKRSTDELLEQSYDKVFGEGDMI
                     RIVLDAALRTNRHVDHHDMPYGTGEQTRTIRVATADLTGDDGQSAGVIVLLKDVTELL
                     DLERRVRVADKLAGLHTLSAGVAHELRNPLSAIDLNLHLLEEELRGGERLSPTRDRYM
                     HVLNAETRRISAILDTFMKFARPGSVPLHPIDTKQLLSHIISLLQYEAQERRIQLITK
                     APDTLPPLMGNETEISQVLVNVIVNAFQAMPDGGVCHVLTRGIKTDEKDWVELSVQDS
                     GIGIKKEDLSRLFEPFYTTKTGGSGLGLAIAYRIVQDHEGTIQISSQPGLGTTVVVKL
                     PAASVTQQALVPDR"
BASE COUNT         6002 a         8302 c         8255 g         5919 t
ORIGIN      
        1 gtggaatctc tgcgtggatt ggctgccctc atggtggcga tttgtcacag tcagttggtc
       61 tttgttgttg atggccatga agatctttgg tgcctcaaca tcgccgatgt ccaggggctc
      121 caggcattgg cgaccaaagt cgtattggca ctattcaacg gcggggcggc cgtctctctc
      181 ttcttcgtga tcagcggtgt ggttttgggg ttgtcattag acaggcagaa ggatgagatt
      241 gtgcgaagca gcgtctcgtt tctcatccga cgggcgttga gaatttatcc cgcgctgatt
      301 ctttctctct tgctggttca agcttggttg ccgctgattt accctgcccc tgattacgtg
      361 ggcgcgagtc tcatgttcca gcctttgtac cgtgttcccc aggcagccga tttctttgcc
      421 aacctgtttt tcgtcagtaa tcaaatgaac cctgtaattt ggacactaaa gatcgagatg
      481 gaagtggcgc tgctgttgcc gatccttcac ctgattaacc ggagcattcc tccaagtggc
      541 aacatggcgg ttctggcgtt actgatgtgg ttgtctacca acgggcacga caccagcacc
      601 gaaaaatggg tgtttgcgtt ttacctcggg ttgatgctcc ccgaatgggg gccttggttg
      661 gtctcggcga tgaggacgtc tcccgcgggc ctcgaaatgt gcttagggag cagcctggtc
      721 gcctttggtg ccgcgcgcca tctactcttt ggaacgggat atgccgtcat cactcccttt
      781 gtcgagggaa tatgcgccat gacgcttctg gcgtgtgtcg tctatcaacc cgcattgaag
      841 tggtggcttg tgctggattg gagaggcatt cgtgccctgg gacgcctgtc gtacagcttc
      901 tatctgttcc acttcatcgt tcactacctg gtcggcgtcg gactgttccg tctagtgtcg
      961 acccaggcat tgtcgaccta tccaatcgtc tggcatggac tgctgggaat cgtttcggtg
     1021 gctctgaccg tgcctctcgc ggttgtctcc tatcgatggg tggagaaggt ctttatcgag
     1081 ttggggaagg acttaacgag acgcatagag gtttctttcc gtcggccgat gccagagggg
     1141 gtgcgataaa gttatcacct cggtggtctc caggcacagt gtcacatata atgaaaagcg
     1201 tgcctcggag ttcagtcgat aaggcgggga aaaagacttc gtgaacaacg tggcaggagg
     1261 ctatcaacga tgaggctcgt gcggttattc gttctcgtcg tgatgattgg aggctgggct
     1321 gtaacggtcc aatcaaaaga tctctcgccg cgtgaaatct acgaacaggc ttcgcccgct
     1381 gtggtcctgg tcatgggaca ttcagaaggc gggagaaccg ggagcggcgg gacaggctca
     1441 attattcagg ccgacgggct ggtcctgacc aatgcccatg tggtcatcga agagcgcaca
     1501 gggaaaccct atccacgcct ctctgtgttt ctcaaaccgg cgcgtgtgac cggcgattca
     1561 aaggtcgatc tgtcccggat gatgcgcgcc agagtcgtcg cgttttctac gccgctcgat
     1621 cttgccctcc tgaaactcga cggagcaccg tccgcactcc cagtcgtcgc cttcagcgag
     1681 tcatcgcagg agcgaatcgg cgaccgagtg gtcgccattg gccacccgga acaggggggg
     1741 ctctggacgc tcacgaccgg tgtcatcagt gcagaggtcg ataacttcaa tggagtcccc
     1801 ggcaagcatg tgtttcaaac agagaccgga ctgaatcgcg gaaattccgg cggacccttg
     1861 ctggatggtg aagggcatat ggtcggggtc aatacagcca tcgtccgtgt ggctccagac
     1921 ggactcccga tcacgagcat cagcttctcg ctcaaatcca acgtcgctgc gcaatggctt
     1981 cgggagcaag gcgtcggtgt gaaggcggct cgaggagtga cagtgccgac gggacaaaca
     2041 agtccgatga cagacgtgcc ggccgcgcca tcagttccgc tgccaggaag agccgttccg
     2101 gttcccaaac aggttgcacc cagtcaacca ccggcaaaat ctgatcgctt ggattcgacg
     2161 ccacatcgcc cctatgatct cgaccagttg gtcagcgacc gtgcaaaggc ggaagcggac
     2221 cttgaaggga tgatgtcaga aatgcggggg aggatgaagg ggaagtaagc atgcaggatg
     2281 ctgtctcgac ttcatgttca tgaatccacg caccagccgg ccgctgttcg ttgtcgttgc
     2341 gctctgctgg cttgtcgttg caagccctgc cacaagctcg gccaagagcg acctcgatcg
     2401 ttttgaattg gtcgggtttg ttcgtgccgt tgtgaccaag cacccccagc tcaagactac
     2461 ccaacagttc gatcacgacg gcaatctgac aagtctggaa ttggttcctg caaaggaagc
     2521 aaactctgcc cggtatgtgt atcttcatga tgcaagcggg cggattactg aggaacatac
     2581 ggttgaccct gatggcacgg ttaccaaccg gaaacagttt cggtatgggg ttgatgaaca
     2641 aggaaggcag ttggccctcg tcgcagtcac cgacgagggg ctctttgccc atgccgagtt
     2701 ctctgtctac gatggtaggg gactcctggc cgaacaaatc atggtttccg ggcaggggat
     2761 cgccgaaaaa agcctgtatg acgttcgagg caatctcatc tacgatgcga ggtactttca
     2821 aggccgcctc gttttggaag cgacacacca ctatggcccg ctcgacaggc tgacggaaag
     2881 ccgattttac ggaagcgatg gcgagctgat gcgaaaagat ctctatcggt acgatcaagc
     2941 cggcaatcgg gtagaacaaa cgagcgaatt ctaccaccag tcacacctcc ggaaatcggt
     3001 cgtggcttac gagttcgatc atatgggaaa ttggacgaag gaaaccatcc agcgctggtc
     3061 ggacaagaat ggtgccccga cgctgggcga gacggtggtc agccgtgaac gaaccatcac
     3121 gtattactag aatggtatag gggtggggag atggacgggg gatcaaccgg cgcgtcagga
     3181 agtcggagtg ggaagacgtc taatgctcaa ttgctcggtg atggcttgct ctttgctgtg
     3241 acttcccata agggtccacc aacccgttcc agatgaaact ctttcctgat ctcggctgaa
     3301 tggtaatagg gatcccaatc atcggccaga tagaggattc tgccttttgc gaccacctgc
     3361 tctctgagga aatccatcgt tgcggctgtg ggatcgctgg aatagtggct ccaatcgtcg
     3421 atgtagatgc cgatctcaag atctttccgc tgacccatca cccgctgttc gtagaccaag
     3481 acgagtccag gattaaaatc cgccaggatc aacgcaccgg ttttggcttg gcgaaatgca
     3541 tcggccgcat actcctgcgg gccacgctcc ctgttcttgg gaggaaagag gtaatacgaa
     3601 taggagttgc gatagggcag ggttctgatg aaagaaacgt cgagctgaac agcctcagcg
     3661 attcgtgagg cagagtagta gacggtcgga ggacacagga caagactggc caggagcgcg
     3721 gagcagattg gggtgcttct cagtcgggga aatttgtcga gcaggtaact tgtcccggcg
     3781 cctacccaca atgcaaacat gctaaaggtc acgatcagca tcggaaattg ccgctgtttc
     3841 atatactgag aggtaaacag gaggatgggc agtgcgatca ggactgtcga taggatcagt
     3901 ctcggcgatc gctgaaatcc tataacaagg ccgagcgctc ccagcacgat tgcgaatccg
     3961 ggaaattggt acagaaggta gaagggggag ccacctaact ccttgatgag ttgaagaaag
     4021 gttcctgaaa acgtctggta ggtattggaa gtgtcgccga cgagttgcgc ggcgaatgct
     4081 gtgaggctgc cggatgccat gattggaatc aagaaaatct gacaggagcc cgccaggaat
     4141 gcaagaaaga cgacggcgat tctccaggag aacatgaact cgaacaacgc tttcccccac
     4201 aacagggcga gaaagccggg gaatgccagg acgagcaacg catggttggc aagcccggct
     4261 cccaccgtgg cggcgaggac atagaggtgc caatctcgtg gctgcttgat ccacttaagg
     4321 cagagcaaga aggccagaag ggtgaaaaat gacaacagcg agtagctctc attgaggaca
     4381 gcatagagcc agaaaacatg actgaccgcc atggatgcgg cggccgcgag agcagcgaag
     4441 agatcctcaa aggtctccat gataatacgg aagaccagat agactgccgc tgcagcaaac
     4501 caggccgata acaaattctg ggaataggcg aacgagaagg gcaagaaaga gaaaatgtag
     4561 cctaagacgt tatgcagcgg gtgagttccg tacccggggc ccaggccgat gaaatgatga
     4621 tcgagcacat aaaacagcag cttggtgggg tcgccccaca gatagctggg ggcaagcgtt
     4681 gaaatgtaga gagtgagcgc agctacgaaa actatgaccc caacgatgtt ttcccgttgg
     4741 ggtcgggaag ttctaccagt gccctgcatg cagaaccatg agaacaccta tgtggtgaca
     4801 gctccttcgg acgccgacga tacatgccgg gcgtatttgg ccatcacgcc tgaggtatag
     4861 cgggggagcg gctgcttcac cttcttcagc ctggccataa tctctttccg ggacaagctc
     4921 acgttgagct ggcgcttggc gatatcgaag acgatcgtat cgccgttttt caccgcggcg
     4981 atcggcccgc ctctaactgc ttccggagcg acatggccgg ccatcaggcc gtgggtggct
     5041 ccggaaaatc tcccgtccgt caataaggcg acggaatcac ccagcccggc gccgacgatt
     5101 gctgccgtca caccgagcat ttccctcatc ccgggaccgc ctgacggtcc ttcatagcga
     5161 atgacgacga catcgccggc cttaatctgt ccggctttca cggcgacaaa ggcgtcttct
     5221 tcacggtcat atacctttgc cggtccgcta aagtgcatca tcgagtgccc ggcgactttc
     5281 actacgcagc cctcaggcgc gagattgcct ttgaggatca ctaatccacc ggttttcttg
     5341 attggattcc cgagcggccg caagacttgt tgcccgggcg tttccgtcgc gcgctgggct
     5401 tcctcgccga tcgtccgtcc cgtgaccgtc ggttggttct ggtgaaggag gccggcgtcc
     5461 aataagcgtt tggcgacgag ggtcgttccg cctgcggcgt acaggtccgc tgcagcaaac
     5521 cggccacctg gtttcaaatc ggcgagcaag gggacttttc gattgatctt gtcgaagtct
     5581 tcgatcgtca gcttgatgtc ggcttcgcgg gcgatggcca gcaagtgcaa gaccgcattc
     5641 gtcgatcctc ctgtggtcgc gaccgccgca atcgcattct cgagcgattt gcgggtgatg
     5701 atctggcgcg gtcgcaggtc attcttcagc agctccatca ccatcttccc gcattcgaac
     5761 gcgacatcgt ctttccgatt gtccatcgcc gggacaccgt tgaggcccat tggagagatg
     5821 ccgagaaatt caaaggcaat ggccatggtg ttcgcggtga attgtccccc gcaggccccc
     5881 ggcccagggc aggcatggtc ttcaaggtcc ttgagctcgg cattcgtcat cctgcccgat
     5941 gcgtgtttgc cgacggcttc gaacacgtct tgaatcgtca cgtcatgacc ttggaactgt
     6001 cctggcatga tcgagccgcc gtagagcatc aacgacggca agttcaaccg cgcgagcgcc
     6061 atcaccgtcc cggggatcgt cttgtcgcag cctgacaacg cgacgacccc gtcgaagaga
     6121 tgcccgcgag ccacgagttc gattgaatcg gcgatgacct cgcggctgat caacgaggcc
     6181 ttcatccctt ccgtccccat cgaaatacca tcggagaccg cgatggtgtt gtattcgatg
     6241 ggagtcccgc ccgcggcgcg gatgccggct ttcacgcgct cggacaaccg gcgcaaatga
     6301 aagttgcagg gcatgacctc gatccaggta ttggcgacac cgatgatcgg acgagacaag
     6361 tcctcatcag tgaagccgac cgccttcaac atggcccggg ccggagcccg tcctggaccg
     6421 accaacaaat cgtggctctt gagcttcaac tccttcggca tgatcgcgtt actttccttt
     6481 cttgccttcc tttttctctt cgtgcttctc gtgtttcttc gcctccacgt ctatgaccgc
     6541 gccggtggcc gcgtcgatat ggacttccga cactttgccg tctgctccca ccacttccac
     6601 ttcccagacc gttttgtcgt gtttcttttc gagttcggct tcgactgccg tgccgggaac
     6661 tttttccaag gccgtcttga cggcctgatc gatggtcact ttggcatctt tgacgagcgc
     6721 cgccacgtcg tcttccccat ccttcttctt atcgctccag gccggagaag cgactgccaa
     6781 cacggttccg atcgcgaggg tgaggactaa attgaaaagc ttcacggggc acctcctaga
     6841 gtgacaggtt gagcactagt ttacggacat gctgcttggg ggagcgggaa cgctgccgtc
     6901 ggagcctttc tggggtgttc catgcggact gacgccgtgc ggattcatca tgccgccgcc
     6961 atgcgggccg gcaggggctt tcccgccctc cttatgttcc tgcatcatct tgtcgtgttc
     7021 ggccttttcc ttcgcgcgct gctctggcgt caagaggccg aggacttcgc ggcgtgtctt
     7081 gatggacgtc atgcgcaacg caacctgcat gtcttcgctc tgcttcaact tcgcttcgat
     7141 ggcgccgagg tcggatttct cgtcgtccgt gagcgctttg agctcccgtt cggcaatttg
     7201 gatatcggct tccgccttaa tgcgcgcctt gtcgagattc aattgaatat tcttgagctt
     7261 ggccacctgg tctgccgtca ggccgatgtc cttctcgtgc tttaagagat gccggatcag
     7321 atgtccggcg ctgctgtgca tcatacccat gccatggccg cccattgcgc tgtgaccgcc
     7381 gcccgaatgc ccgtccttcc cataccccgg ttcattagcc cagatagccg gaagtccgag
     7441 catcagcgcg aatgccgaca cgatgccgag cgtggacatt gttttggttg ccttcgtcat
     7501 gccgtcctcc ttgagttgat ggagcctctg cagatggaat taatcattat cgcacaaaca
     7561 aaccgccttc gcccttcagc ccgttgaata gaaactgaac ggctaaggcg gccaggagta
     7621 aacccatgag gcgcgtgatg atcttctccg ctaatgagct catccacttg gcgccggacg
     7681 cgccgactgc caagattgcg tagcttgcca gagcaaccag cgcaacgcaa gcgagtaaga
     7741 cgcctcgata ggtccaggat gcagcctgag cgtccagcag aatcaccgtt gaaatggccg
     7801 ccgggccagc cagcatcggg acggcgaggg gcgtaatcgc aatatcatct tttgttgcgc
     7861 cctccgccgt ttctgccgcg gtttcttgaa cgggtgagcg ctgcgcccgc aacatgtcca
     7921 gtgacaccaa caacaagacg agcgctccgg cgacttggac ggccgggagc gtgatgccga
     7981 gcagcgaaaa cagcgattgg ccgacgaggg caaaccccat caagatcccc atcatcacga
     8041 cacaggccgt gcgagccatc cggagccgct ggttcagagt atctcttgca gtcatggcca
     8101 gaaaggccgg gacagtcgcg atgggatcga cgatcacgaa gagcgaactg aaggccagca
     8161 cggcatactc ggtctgggtc atcgcggtta ccgcatgcgc gtctgattga gaagctcggt
     8221 tcgcgggtcc ggatgcttcc cgcgtcccaa gcgttcgtag agcgtttgtt cctcttcggt
     8281 aagggaaggc ggcatcacga tttggagcgt caagaacaag tcgccatgac cgccgattgc
     8341 agaggggagc cctttgcctt tcaatctcag ttttccctca gcccggctcc cggccggtac
     8401 tttcacgcgg accggttccg tcaaggtggg cgccataacc tccgcaccaa gggcggcttc
     8461 ccagggatag accggaagca tcacatggat gtcgctgcct tgccgccgaa agacggggtc
     8521 cggcgggatc acgacatgca ggtagagatc gccgcgcttg ccgccgttat ggccgggctg
     8581 tcctttccct gcgacacgca cgcgtgtgcc gtcctgaacg ccggcgggaa tcttgacctc
     8641 gatcgttttc gcctcggtcg tcgagccgct cccaaagcag gtcgggcagg tccggttgcg
     8701 gaacgctccg gtaccctggc aggtcgagca ggggaggggt tcccgcagac tcacgcgttt
     8761 cgtcacgcct gtgagcactt cccgcaaggt cagatcgact tccgtctcta aatcatcacc
     8821 ggggacggca aacccgcgcc cgcctcccga cttgcctcgc cccccgaaca gattttcaaa
     8881 gatatcggag aacgcctcgc cgccaaatga accgccggcc tgtccgccgc cgaacccctg
     8941 ggcgcctgcc tgctgacgtg ctttctcgta ggcctcggcc tgttcccatt gcgcaccgta
     9001 ttgatcgtat tttttgcgct tatccggatc ggataggact tcgtgcgctt cgttgagttc
     9061 cttgaatttc ttctccatct ccgctttttt tgagcccgag tggaggtcag gatggtattg
     9121 tcgggccagc cggcgatagg ccttcttgat gtcatctgcc gtggccgtgc gtgagagccc
     9181 gaggatctga tagtagtcgc gcgccgtggt tgccataagt ctcgagataa cgttgggatt
     9241 tggagaatca acggtgcttc gcgcagctta cggggtcgat ggagcctttg caagcaccgt
     9301 cgaatcggct cacagggtgg tgggagtttt tgcccgagcc atcaatttgc aataggaaca
     9361 gacctccgaa gtggtgggct gcttgcagat acgacaagga tgcagcaaag cttgatcttt
     9421 ttctgccatg gtcgtggggg cggattcctt cttgctctgc ttctcgagaa acccccagta
     9481 gaacgtctgt ttcgtccctg gcgactcggt ttccaatcga ttcaagactt ccttatagag
     9541 caaggatcga gcgccctgcg ccatcggaca ttcgtccacg atatagtcga tcatgttgag
     9601 gacgcagtag gcagccagct cgcgctccgt caaccgatag aggggcttta ccttcttggc
     9661 aaacccttcc accgaagcgg ggagactcgg gccctgttta tcgaggtact cttcctgcca
     9721 atgcaggacg ttgccgagca ggcgggccgc ttcgtcatcg agattgtggc cggtggccat
     9781 cacatcatac tcgtgctcaa tcgccgcgcg gttgaactga tagcgcttga tggttccaca
     9841 cgtactgcag gtcgggcgat ggaccagttg cgctagctcg cgaatgccgg agccctcctc
     9901 ttgttccacc ttgtgggtat ggagtgtagc gccaagtggg gccgcgacgc tgtcggcgaa
     9961 tagacggacc ttgtcgtagg atcgatctga atagccgccg atgccgaggt tgacgtaaag
    10021 cgcatcggcc ttgtatccaa gcttcaataa aatatcccag agggccagac tgtctttccc
    10081 gccggagacc gcgaccagaa tccgctggtc ctttccgaac atcttctgcg acttgatcgc
    10141 cttggcaacc tgatcgtgga cgaagccatt aaaacagccc ttgcagaagg ccgcatggtg
    10201 gcgcgggagc ttgatgaccg ctttggtttt acacttggtg cagttcattc ttagggcaag
    10261 caatcagctc tcggctgtca gcgttcagcg ctagagcggg actctttgct gaaggctgat
    10321 ggctgaccgc tatcctcctg agatgacagg tcggatttcg atctggtcct tgtcgaagag
    10381 catctcgtcc tcggtcacca gttcgtcgcc gcggatcacc aggtgcgctt cgacgacaag
    10441 gctcagctcg cgcaggacgt ctttggcccg cttcggcccc ttgatttcaa cggttctggc
    10501 cgggtggctg agctggactt gcattgacgc gatgatacgg ctggctcgtt gtgaattgca
    10561 aatagccgcc tgctgatgcg cgaccggccc ctggctatcc ttgaaaggag ggtgaaacga
    10621 ctgtggttgc tgctggttta cttttcggac gattgccgtt gctcccggag attttcaagc
    10681 gccttcaaga tctcagccgg acgtggcggg cagcccggaa tgcggacatc cacaggaatg
    10741 tgtcggtcga ccggtccggt tactgcgtag cttcccttga agactccgca gtgaatggcg
    10801 cagtcgccga gggcgatcac tattttcgga tcaggcgtct gtttgtgcac atctttcaac
    10861 gcgtgttcca tgttgaccgt gactgggccg gtcacgacga gggcatcggc atggcgcggc
    10921 gaggcggcga tgtgcatgcc gaaccgttcc aagtcgtaga cggggttcgc caatgcgttc
    10981 atctccatct cgcaggcgtt gcacgagccg gtatccactt cgcgaatggt caacgaccgt
    11041 ctgaacggcg aggcttttcc acgtacctcc ggtgcgagct gttcggctgg ttgcttggcc
    11101 tggggatgct ggcccgttac gactccggtc ttgagactct tcttgagaat acgaaacatg
    11161 cttcctttct cgtgaggcgt gaagcgttag gcgttttggg acagaaatta cgccgacttg
    11221 ctgtcatgct ccttacacct cacgcctcac ccctcacggt cacagatcgt tccccgcata
    11281 ggacaaatta aaactcttat tgatcaatgg aaaatcggga atgatgtttc ccacgacagc
    11341 ccattggatg gcgggccagt tcacgaacga ggggtctcgc accttgcacc gatgaatccg
    11401 accgttctca ccggccatca cgaaatagag aatttctccg cgccagcctt ccacggaaga
    11461 gagcgcccac tcgccagggt ttggtgaacg aaccggcgct gcgacgagcg ggctttgcgg
    11521 aatcttgttg cgaatctggc ggatcagccg aatcgaatcg tgaatttcgt ccaggcgcac
    11581 gcgcaatcga gcgcggacat ccccgtatcg atagagcgca acgtgaggcg gaaactcgtc
    11641 ataggcggcg aacgggcgat cccgccggac gtcctgatcg atccccgacg cccgtcccac
    11701 cacgcccatc acgccgtgat cccaggccgt gcgttccgtc aggacgccgg tggtttccaa
    11761 gcggtccgta agcgaggcgt tcgcgaagat gatggactcg atttccgaaa aatccttttc
    11821 gatctgatcg agcagatcga cgatttcgca tcgttggtgg gccggtatgt ctctcgcgac
    11881 tccgccgatc gtcatgctct ctcgcagaaa tctggagccg gtcagccggt cgttgagctg
    11941 catgacacgt tccttcatcc ggctgcagtg ggcatggggg agcgcgtacg cggtgtcgtt
    12001 gcagattgct ccgacgtctc cgaggtgatt gtgcagccgt tccagctcga gaaagagcga
    12061 acgcaagtat ctggcgcgtc ggggcacgtc gatgccgagg agcgtttcca ctgcctggca
    12121 gtaggccaaa ctgtgcccga ccgtcgtatc gccggacacg cgttctgcga gcggcaccgc
    12181 cgtggtgagc gtctgttgtt cgaacagctt ttccactcca cggtgtttcc agaaatggcg
    12241 gacttcgagt tgcatgatcg gctcgccggc aacggagaac cggaaatgcc ccggctcgat
    12301 aatgccggcg tggatcggtc cgaccggcac ttcgaagacg ccctcgccct cgatctgacg
    12361 gaatgcatac tgcccctggg cccgctccaa cacggtgtcc cattggaagt cttttttgag
    12421 cgggtgggag cctttgggcc agtgttcatg gcggacgagc cggcgcatgt ctggatggcc
    12481 gacggggatc agaccaaaca tgtcgcggat ttcccgctcg taccacttgg ctgcatggat
    12541 gtggggagta atggacgcga acagacgatc atcaccccga aggtccgtgc aaagcagcaa
    12601 ccagtctttg cgctccgcca gtgtgaacag gtagcagagt tcgtagcgcg cctcgtgcgg
    12661 ccgatggtcc acggcccaga gaagggacaa gactccgcgc agcccaggat ggatgtggag
    12721 gtagtgcgcc accaccggca catcttcctt cttgatccgc agtcgcggcg tgccgtggac
    12781 gggaggcatc tcctcgatgg agttcccata cgcctctttc aattgtttca cgcccgttat
    12841 cgcgtcaacc atcgcttcac ctcgcaagca ggacagtgac cgctcgatct aggagcgttc
    12901 tgatcggatc ggggagcgtg ataccaagtc ccactaaggc gagactggtg aagatcagcg
    12961 gcacatgccc aatatcccat ccttcaccgc gcttcacgct ctcctgaggc gtcccccaca
    13021 ccatggcagt gagccgataa gtaaacccgc cgaagagtgc gactgcaaac aaaaggaaca
    13081 ccgtcacgat cgacagactg cgtacgtcat cggctacgac gatggtcaga aaacggccca
    13141 gatggagcgt gtccgaggca aaattttggg tcgccagcgc cgacacgacg agtacctcgc
    13201 ttacgaacat tgagaatggt ggcatgccga ccagggccag gcctgcgata aggattgcca
    13261 ccgccgtgac cggttgggtt ttcgccagtc cccgcacctc accgatctca cgggtcgcga
    13321 accgacgatg gacattcccg gccgcgaaaa acgccagtga cttcgccaac gcatggttga
    13381 gcagatggaa caggcccccg aatgttccaa ccggtccgcc gactccgaat ccgaccatcg
    13441 ccaaccccat gtgttccaca ctggagtagg cgaacaaccg tttatagtcg tgctggatca
    13501 ggatgaagag tgcggccacc acgaacgaga gcagcccgaa gaggagcagc aggttgccag
    13561 agaaggccga ggggagcgcg tgatccatga tcgccttgct gcgtagcacg gcgtagactg
    13621 caacggtttc cagtacgccg gccagcatgg ccgtgaccgg cgccggcgcc tcgctatagg
    13681 cctccggcac ccagctatgc atgggcacca gcccgatctt ggtaccgtag ccgacgaaga
    13741 gaaagatgaa tgcgagcttg agcacatgcg gatccagttg accggccact tcaaggagtt
    13801 tggtcacgtt caatgccgcg ctctcatctc ccaggacgcg gaccgacgaa taataggtca
    13861 acaccgtgcc gaacagcgcc aacgcgatac cgactgagca gagaatcagg tatttccacc
    13921 ccgcttccag cccctcacgt ttgcgaaaga atgcaatcag gaacgtcgtg gcaagcgtcg
    13981 tcccttcgat ggccacccag agtacgccca ggttgttcgc catcgtcgcc acgatcatgg
    14041 cgaacagaaa gacatgaaac aggaagaaaa accggctcag gcgcctcggc gcaatcacac
    14101 cttgagccac gtaatcatcg agataggccc acgtgtacaa cgagcaggac aatccgatga
    14161 ccgtcacgat gaagaggatg aatgcggaaa gcgcgtcgat atagaccagc ccccagagcg
    14221 cggtcagggg tccgtgagcc agcacctgct gaataagggc gatttcagac acgaccagac
    14281 cgatcatcgt ggcgagattc aacccgtgca acacacgggc gtgcctgatc gccaaactga
    14341 aaaaggctgc gagcagcggt gcgccgagaa agaccgccac cggcgcgatg atcgtctgca
    14401 tcagggggcc tccatcccga ccctgtacat cttgacgatg gacgccagca agatctccag
    14461 gcccggtgga acgcgaagcc cgatggttcc cagcacggcg atgttgattc ctacggcagc
    14521 gctcgtaaag aaacggcagt gtctgctcaa cgcggtgcct gcctcccccg gcgctccaag
    14581 caccatccgc aacacctggt agagcaaggt tgcaaaggcc agcgcaagga gagccagaaa
    14641 cccgtaggcc acttcaggcc tggcggcgta ggcctcagcg gcaatcagca cttcgctggt
    14701 aaagagtccg aagggcggca tgccggccaa cgcgacaccg gccacgagga tggcggtccc
    14761 tgcgaccggc atctggccta atagcccagt cacgcggtcg agcagtttgt ggttgtgccg
    14821 aaggaatacc agaccagcgc catagaaggc cgtggatttg gcgaacgcat gattcatcag
    14881 atgccacgcc cctgcaaaga ccccaaggcc gccgacgcca aaccccaaca gcgcgatccc
    14941 gatgtgttcg atgctggagt atgcgaacag ccgtttgtag tctcgttgga ccagaaagaa
    15001 agtcgctgag atggcgatcg acagcagtgc caatccgatg atcaggttgc cggcaaattc
    15061 gggcccgacg gttctatccg tcacggcctt gaatcgcagg atcgcgtaga cagcgagcgt
    15121 gagcatgttg gccgacagca tggcactcac cggtgtcggg gcttcggcat gcgcatcagg
    15181 cagccaggtg tgcatcggcg cgagtcccgc tttcgtcccg tagccaatca acgcaaaaat
    15241 gaacgccact ttgatcgcac caggatccaa tccctcagcc acgcgataga gttcggacca
    15301 gcgcaacgcc tcactcgaaa cgcccaacac atgctccgac gagtaataca cgaggatggt
    15361 gccgaacaac gcgagtgcaa tgccgaccga gctgagaatg acgtatttcc agccggcttc
    15421 cagcgccgcc ttactccggt cgaacccgat caacaacgca gccgagagcg tggagccttc
    15481 gatggcaatc cacatgatcg cgatgttttc gagattcacg gccaggagca tggcgccgag
    15541 gtacagatca aacaggcaga aaaactggcg gtagcgtctc aatgacagat gtgcgcggtc
    15601 cagttcttcg cccacgtacc ccacagcata gagcgccccg gtgctcccca cgccgccaat
    15661 gatcaaatca agccatgcac tcagcgcatc tgcctgcagg aggtttccca cggagacgtg
    15721 tccggcggcg atcacccgct ccgccacgag cgcggcagac gccagcatcg cccctgccag
    15781 tccgcattgc aggacctcca cggctcgccg ctgtctgacg aggagactgc cgaaacctcc
    15841 gatgagagga gcggcgacca gcactagaag cggccaagcc atctcgcgtc attccttcag
    15901 gatggtgagc ttggtcgtat ccacgctgtc aaaggcgtcc tgtagccggt gtgtatagat
    15961 gccgatgatc agagccccca ctaacacatc aaagaagaca cctaactcca cgatcagcgg
    16021 catcccgtac gccgctgccg tcgcccccag gaacaagccg ttttccatga ccaggaagcc
    16081 gatgacctgc gtcaccgctt tcttgcgcgc gatcatcgtg aaaaatccga tcaggatgat
    16141 cgccagtgcg atcgccaacg aatcgcgcgt cagcaggaac cccaggggaa tgatgggctg
    16201 ggtgatgaag aacgccagta tcaccaatcc gccgcagatc atgaggccgg ctgggacgtt
    16261 cacattcatc accaactcgc gtgtcacatt cagccgctca atcacctgct tcagaatccg
    16321 agggatgacc acggccttga tgacgacggt gagcgcagcg gcgatgtaaa tgtgatgaat
    16381 ccccgtgagg aatgcgacaa gggctgccgt cagggccaga aagaccgact gcagggcgaa
    16441 caagtcgaca caggcagaga gcctgcgctg ggccacaatc gcaaagcacg tcaacagcaa
    16501 cagcgccgag cccagatcga ccatctgaga tccgatgtga ggagtcaaca gcggcacgtc
    16561 atcctcgcaa cacgtagaag aaaaccaacc caagcagcgc cagaataaaa gcgacgccca
    16621 gcaagtccgt cactctgaac aatcgcagct tggcaaacat cgattcaatg acgccgatca
    16681 gtacggcgag cgctgagact ttggccagat agacgacgac acccaggccg agcgcggcgg
    16741 gttccaggct tgtcgcaata ccccacggtg cgaagacgtt cacgatcaag gtcaggaaca
    16801 ccagcaactt gagtccggct gcccattcga ccagcgccag atagcgtccc gagtattcca
    16861 agatcatcgc ctcgtgaatc atggtcagtt cgagatgcga ggccgggttg tccaccggca
    16921 cccgccctgt ttcggcgatc gccacgatga agagcgcggc aagcgccatc agatgcggag
    16981 acggatcggt cacaatacct tccagcaagg ccgtcttatg gacgatcgta ctgaggttcg
    17041 tagaccccgc ggtgagggcg atcgcgaaga tcgagagaat catggccggt tcggtcagcg
    17101 aagcgactat ggcctcgcga ctgctcccca tgccgccgaa ggccgagccg gcgtcgagtc
    17161 ccgccaggat cagaaagaac gtgcccaacg ccagcaggta cacaagtgcg atgatattgc
    17221 cggcgaaagt cagcggcgtt ctggacgcaa acactggcac caacagtccc gccgtcagcg
    17281 tcgaggcaaa cagaatgtag ggcgtcacag taaagatcca cgaggtggtg gtcgaaacca
    17341 ccggctgctt ccgaaatagt ttggccaggt cagcgtacgg ctggaagacg ctggcgcccc
    17401 gccttacctg aagccgggcc ttcactttcc tgatcagccc cacgatgaac ggtgagacgg
    17461 ccagtaaaac aatcgtttgg gccacaatga gaacgatggc ttgcaacatc ggcttatacc
    17521 gcgatcaaca gcaagatgac caacgtgaca aagatatatg tcaagtacag gtgcaggctc
    17581 cccgcctgga tcaccctcag gcggccagcc atggtcgtga agaaggcgac cacgggatca
    17641 tagagatact tctgaaaaat cggttcaatg tgaaactcga agcgtcgccg cttggcgaaa
    17701 tagcgcgact cctccaaaaa ttcggtctcg agcttgaccg tcggttggta gatggtgctg
    17761 aacactcgtt tgatcggttg gacgaagccg gttgcggtgt attccatgcg gggcgtgagg
    17821 ttgatcccac agccccaggt cttgtaatag cgcttcttca ccagaccacc gaacgttgcc
    17881 acgagcccca acccgagcat cgacagcgcc accagcagga acgccaacac cggcgtcgag
    17941 atgctggaaa attcgacatt caccggcgcg aggacccatc catccatcgc caacaccttg
    18001 tcctcgatcg acacccctac aaagggcgcg gtcactcggt ccaacagtgg taccgccacc
    18061 atcggtgcga cgcccagcac gatacagacg atggccagca gagccattcc aaggcgcata
    18121 gaaaatggaa cctcctctgc gtggcgggcg tgcgagcttc gtggcaatgc gagaaatgaa
    18181 atgccgaatg ctttggcgaa acaggtcagg gcgagcactc cggtgagcgc cagcatggcg
    18241 gcggcgatcg ggagcatcag cttcatcagt aaggagggca tctggaagct caagaagagg
    18301 ctttggaaga ccagccactc gctgacaaac ccgtttgtgg gcggcaatgc cgagatcgac
    18361 accgcgccaa tgagaaagaa cagaccggtc cagggcattc gacgcagcag gccaccatac
    18421 tcctccatgt cgcgtgtatg cgtggcatac agcagtgagc cagcgcctaa gaacagcagg
    18481 gccttaaaca tcgcgtgatt gatcgtatgg tacagccctg ccagcagtcc gagggcggcg
    18541 agttccttca gtccgtaggt ctgaaagatc atgccggccc cgatacccaa cagaataatg
    18601 ccgatgttct ccacgctgtg gaatgcgagg agactcttca tgtcgtgttc catcaacgca
    18661 tacatcacac ccagcaacgc tgaaaccgcc ccgatgacga gcacgacaaa cccccaccac
    18721 caaggaaact ggcctccgag aaaatcaaag tacacccgga tcaaggcata gatcgccgtc
    18781 ttgatcatga cccccgacat gagcgctgaa acatgggacg gggcggctgg atgcgcatag
    18841 ggcaaccaga cgtggagcgg cacaatgcct gctttggtgc cgaagccgat cagggccatc
    18901 aggaatacga gagttctcat gccttctggc aaaggctgct ctggatgccg gaagatctcg
    18961 aacgagaacg agccatccgc ttggaagaga atcaagaagg tcaggatgat gaacgcagtc
    19021 cccacatgcg tcatgatcaa gtaaaaaaaa cctgcatagc gcacttccgc cttctcgtgc
    19081 tccgtcacca cgaggaagta ggagaccaac gacatgagtt cccacacgat gaggaagaaa
    19141 aacccgttgt ctgcgagaac gacgagcgtc atcgagccga gaaacgcatt gaagagcgat
    19201 cccagcgccg cgatagacac gtgccctgga aaatgccgca catatccgat ggcatagatc
    19261 gatgcggcga ggcctgcaag ggagatggtc aagacgaaga atgccgccag cgcatcgagt
    19321 cgaatcgcaa acgtgagcag ggggattgtc gaggctatcg aacccacgac gggttccgaa
    19381 gcggtgaggc caaggaaccc gaggacgatg cccgcgccgg ttgccaccac ggcggagatg
    19441 ctggcggcga gaccttgcgc gtcacgctgt cgagggaggc agaacggcag cagcatcccg
    19501 accgcataac cgccgagtag aatccacaag aggttcagca tgcggtcgtt ctggtgtgat
    19561 tcaggttatc gctcctgtcg tgtcaatctg gggacgatct gactctggac atcgttcgtg
    19621 aggccgtacc gggcagagag tcggtcgacc atctgattgg catagagccc ccccggagcg
    19681 ggaatctgcc tcgtcagtca cgccgtgttc agtgatcctg cttcttgatc atcggatcga
    19741 tttctctctg cgtttcctcg gacacgttgt agcggatgta cggatgatcc atcacctcgt
    19801 tggcgtagag ccgcccggtc ggggccggga ctgcgatctt ggcctccacg gtccccttcg
    19861 ggccgatggt gacggtttgc tcgatcgcgc tacgcacata gggatgccag acgaccatct
    19921 tataggtgcc cggcggcaca ttggtaatgg tgaaccggcc ctgctcgtcc gtcttggcaa
    19981 agtacggatt ggtgacggcc acgccccagc tctccatgta ggcgtggaag ccgcattgca
    20041 tgacgaagat ccggcggttc ttgctgagat tgaccaattg cttcatcggc gcgccggcca
    20101 tgtgtttgtg gtacatcccg gcgtcgctgc gatccttcaa gttgcgcggg tgctgcggat
    20161 tcatcggcag cggcacgttg aatagcacgc gcgcacccaa atgcgaggtt tcataagcct
    20221 gaatgtcgtg catgaccggg tccatgttga ccaccgtcac cgactgatcg tcccgcacga
    20281 ctgttgtgaa tgggacgaag aggcagtctc tcgcttcgat ttgcggcacg cctttttcgt
    20341 cgaagggttt gcccttgttg atcccctcca gataaaccac cacgtcgcga aactcgcctg
    20401 caggtccgac gttgaacggt tgtaggatgc gccagccttg cccatcagaa atgcgcccgc
    20461 aataatattg gtccggcaag gtaaccaggt tgtagccttt tggcttcggc accttgccgt
    20521 cgagagtaac cttgcccgtc aaggtgccgc cgtcggtaac ggcgacttct tgataagacc
    20581 aggcgggcga gcctattccc gtcatgattg cgatcagagc ggtcacgaaa tatcgcatgg
    20641 ctttcctctc ctcataagat aagaatagat aataaatata ggtccaaata aacaagcgcg
    20701 caagacatga cgcctctcag gtttacgagg gtcgcgtccg tttctgccga tgcaccgagt
    20761 ccacgatgcg ttgaatcgtg tcggcggtca ggtcgcgaat cgtcacgtgc gcttcgtcgt
    20821 cgccgttgaa tgtgcagagc gcgatggtga agcagtccgt gccgccgcgg atgatcttga
    20881 gcgcgatatt ggactgatgg cagtgtacac cgacaaagag acagacgtcg atcttgtttt
    20941 tccagatggt caggttcgga tggttcgggt ttatctcgtg ctccgccaca atcttcggat
    21001 acttcggccg gtaatcggcc atcgggatga gcctcgccgg aacagcgttc gccaaagcct
    21061 tcaccacttt ggacttgcgc actgcctgtt cgttccaggc ccagaggacc agcggtcccg
    21121 ggaagatcgt cgggttcttt gcctggatca gccggcgcgc cgtttcgtcg atcgcctgtt
    21181 gctccggcac ccgctttccc atgacgagtc cttctccttt cgagggaccg atcacgcctc
    21241 gttctgagac ggagggtggg aggtagcctt cgggacccac tgtcatttcg tacggcttca
    21301 tcttctcaac tccttttctc tttcgtggtg attaaacagc tcccaagctt tctcgaagaa
    21361 agatatccat cggcgtaagc gcttgtccgg ggggtggggt caacacgctc gcgggaaacg
    21421 aacaggctgc ccgtagcctt tccttataac cgtgggtccg gtttgattcg accaggacga
    21481 tacgcttgac ggttctggcg aaggactcca attcctcctt cgggaacggc acgatgactt
    21541 tcggatacag acttgccacg ttgaggccga aattccggca gagcgtgacg gcatctcgaa
    21601 ccacgccttg cgctgctccc catgcgacaa agcccacatc cgccttggtt ccttcctctc
    21661 ggtcgaccat cgcggtgact gggtcaggct gatacggtgc tgctgtgttt cccaggtcgg
    21721 tacttccgcg gcgaatcctg ccgaccaggg aacgaatctc aacgctttgc tggcggcgcg
    21781 cgacagtttc ttcgatcaag accccgacgc cgtcactcag gccgtcgcga atcagggcgt
    21841 gccccgcaag caacagcccg agcgcgttcg aaatgtcggg agcgacgaaa acttttggag
    21901 ccgtgaaggc atcgagacaa gtcccgatag aggaagtgtc tccctgcagt atggccatca
    21961 ccttgccttg tggtcctcga taggcctgca agcccggttg gcttttgctc tcaagcatcg
    22021 atacgatagc gccggccatc cctgtgtggc gggtgaccca ttgttccgcc gactcattca
    22081 actctccgct gcaagcctct ccacagtcgc aggcactaac tgcgaacccc agcatgatgg
    22141 cctgatgggg agtcaggagc acctggctat gggcatctgg gacctgagac tgtggcagtg
    22201 catacgcatc gtgcttttca agcactcgcc tggcataggc ccaccccgct gcgaacgatc
    22261 gaggaatcgc cgccgaggcg gtcaagccgg ggagggcctc ttcctgaata cccaacagct
    22321 gcaagagggc tcccaacgct gcgaacccct tgccgatgac accttcgcca catgaggcac
    22381 agagggtgga gaggggaatg tgatacggaa ccactccttc aggcaggacc ggagacactc
    22441 gcgggtgagt cggaggttcc caaaggaaga cgctgcccgg ctggaggccg aactgccgga
    22501 actccggcag gtgctcactc agacaaatga gcacatcgca tccattcccc atcgagtaga
    22561 acgtccgggt cgcaacgcga atatgcaaca ggtcatcgcc gcggtcaccg ttgaattttc
    22621 cgctttgggc catcaccgca aagtctcgat caatgagaca ttgggcgatc ggcgaccagt
    22681 gcttgagcaa ggctgacgcg cctggcccta cgactttgac gacgagatcg aaatcttgat
    22741 cggaatcagc cggcagccag gccgtcaatg gagccatatc actcgactgc atacctaaac
    22801 acggtaggca tttcgcagct ccttgaggcc ggagacactg agccaacgca cggagtatcc
    22861 gctcagaaaa aaatggtcgt acacggcgga cagaaagacg tggccgtcgt gcggacagac
    22921 cggatgccct tcccagtccc tgagtccccg acgcaacaga gcctgcaatc ctgcatggct
    22981 cgtggcaatt gcgcctgtcg tgagatcaca ccagacgagg gcggttgccc ctctggggta
    23041 atggatgacg acgatgtcgg catgctctga catacgttca gatactcttg cgggttatcg
    23101 gttctttttc tttttggtca tggacttcct gttggatgtc ttggccgtcg ccttgcgttt
    23161 cggtgccatc tggtccctcc tgtgatgaag cgtggtgaac aattcgtccg gtcgcggaat
    23221 cggactccgt tgcccggtgt tctgagtcaa cgggcagcgc tgccactgaa gcgcgcgttc
    23281 gccgtcggcc gggatattcc ggcgaaccac tgccgattca acgagtccta gagcgcaggc
    23341 atgctcttcc cttgatctct tacgccactg ccacccgatc aggccggaca tagatggttt
    23401 ctgcctgcat caagccgtcg cgaccgcggt tgtagctgac ctcgaccaat tcacctgcac
    23461 ataggctcga gaggtcaacc cgttctatcc cccgtgcgat cagtgtctgt ctcccgacca
    23521 aggcccggac tcggatcgtc gttcgcacag acaagatcgc ctttacctca acccacggag
    23581 tgccgtggag atcatcttca atccgactga gacagcccca gatcgtcggc atgggtagcg
    23641 ctctctcact ggtttgactg gagaggctgc cctgttcgcc gtcgacagcg agccaccccc
    23701 tcacgggctg tgaggctatt aggattgcac gctaagaccg ctttccagtg gaacaaggcc
    23761 tcctcatgct gctctaactg ttcaagcatg acggcaagtt cgcatcgcgt cagcacatct
    23821 gcagggcaga gcgtcaacgc atcttgcagc cgaatgagtt gtgtggccca ttcgtcgttc
    23881 gacctgtgga gtccggagca gggcggttcc gcggagaaac cggtgctcag gtctttgctt
    23941 gctccgccga ttttcttgcc tgtcctctcc acaacagctc ccgtcaccta cgcccgactt
    24001 ggatgcactc gttcgacccc gtgattcgcg cggatctggg tgccggtcaa ccgtacggtc
    24061 ttcccgtctt tcggcaatcc gatgccgagt tccttccgga ccgttctgaa aatactgatg
    24121 atatggtcca tctttttcac atccaggtcc tgcacgctca acattgcgtc ctcatgaatg
    24181 tcgtggcaga acgcgacggt gagacagttc gttcccgctc gcaccatccg caacgcgagg
    24241 ttggcatagt gacaatggat cccgatgaac aacgtcgctt caatcttatt tccccatata
    24301 gtgagattgg ggtggttggg attgattccc tcctccggat cgatcttggg atactttgga
    24361 cgatagtccg gcatgggaat gatcatcacg tccggaattt ctgccgccag tttcaacagc
    24421 gcctgccctt tggcaatcca ttcatgatgc caggcccagc aatacagcgg accgggaaag
    24481 atcgtgggat tcttgcgcgt gtagagttgt ctggccgcct cagcgatcac cacatcctcc
    24541 ggcgcatgat gtccatacaa gagtccgaat ccggaatccg gatgagtgac acccatctcc
    24601 attgccgatg gggggtggaa ccccgcaggc ccgacatcga tgatccgttc aagctttgct
    24661 gatcccatgg ggcccatgct tcgttccttt ctgtcttgtg ttgtgatgag ggaaccttcc
    24721 gcgcgttcag tcggctgagt gcccgtaccc ccgaagcact ccgtagactg tggctctgga
    24781 aagtgtgcct cgcacatgac gaacgtcata atccaatgca gggaggatgc cgtcggagct
    24841 ctttgtcgga tttcgatggt ggtgccaaga aaattgcgca cttggaagct tggcttctga
    24901 aactctgcag agcgccgagc cccgtcctgc ccaccggctg caagacttgc agattctccg
    24961 gcagtagcgg ctgtgcggct ctgcaaaact tgcagcgatg cgaatctgcc cgcagattcc
    25021 cgactatctg attggttgac ctgcgcaaca gttgcggatc ggcaggagga ctcttgacgg
    25081 gagactggtg aagggaaggg aacgttactc gcggatgccg tattctttaa ttttgtactg
    25141 aagcgcgcgc aggctgatgc ccaggagttt ggctgccttc tctcggtggt tggtgacctc
    25201 cgccaaggtg cgccggatgg cttcccgctc gatgtcttcc aaggatgagc cgagagtcac
    25261 gaccatcgtg cgcgcatctt ccttgctcgc ttgaatttcc tccggaagat gatccggttg
    25321 gatgacgggg tccttcatcg tgaccactat ccgctctatg aggttccgga gctggcggat
    25381 gttcccgggc caggcatata gccgcaacaa ccgcatggcg tcgcgcgaga cttctttcgg
    25441 ctgccggtgg tgctgggcgg agaactcatc cagaaatcgg tccagcaaga gggggatatc
    25501 gtccccccgc tcccggagcg gcgggagacg gatcggaacc acattgagcc gatagtagag
    25561 atcttcccga aaatctcccc gtttgacggc ttcttccaga ttacggttgg tcgccgcaat
    25621 gatcctggta tcgactttga tcagctttgt tccgcccagg cgcctgaact ctttggtttc
    25681 cagtacgcgc aggaaatcga cctgcgactt gagcgacaat tcccccactt catccagcaa
    25741 caaggtcccg ccgtccgcga gctcgaatcg tcccatcttg ttgttcatcg cgcccgtaaa
    25801 ggcacctttt tcatagccaa acagctcgct ctcgaagaga ttttcaggca gggctccgca
    25861 attcaaggtg acgaacggac cgttggccct cgaacttttg tggtggatcg cccgggctac
    25921 gagttctttg ccagtgccgc tttctcccgt gagcaaaacc gtgacgtcgc tttccgccac
    25981 catctccacc agactgtaga cccgctgcat cggggcgctt tcgccgacca tctgatcgaa
    26041 gcgcgtgcgc gtttcgagtc gatcgcggag ctgccggttc tctgttgcga gcacgtgccg
    26101 ttcgagcgct ttcgcaacca cgacggggaa ccgctcgcga tcaatgggtt ttgtcaggta
    26161 gtcgtaagcg ccgcggcgca tcgcctcgac tgcagtgtcg atcgatccga aggcggtcat
    26221 gacgatggct tccgtctcag gccatcgctc cttcattcgg cggagaaatt ccatgccgct
    26281 caggccgggc atccgcagat cggtaatcac caaatccgcg gaagagtctt cgagcaacgc
    26341 gagcgcctct tccgcggttc ccgctcccga gaccgtatgc ccgagctttt ccaacatggt
    26401 gatgagagcg gaccggatgt tgacctcgtc ttcgacgacg aggatgtgag cgggggttgt
    26461 catcggtcag gtaccagcgc ttgttgtgtt acagaggcgg caggaagttt gacgacgacc
    26521 gtcgtgccga ggcccggctg gctcgaaatc tggatcgtac cctcgtggtc ctgcacgata
    26581 cgataggcga tcgcgaggcc caatccgctg ccgccggttt tggtcgtgta aaacggttca
    26641 aagagacggg acaaatcctc tttcttgatg ccgatgccgc tatcctgcac ggagagctcc
    26701 acccaatcct tttcgtccgt cttgattcct cttgtgagga cgtgacagac gcctccatcg
    26761 ggcatggctt gaaacgcatt tacgatgacg ttcaccagga cctggctgat ctcggtttca
    26821 ttccccatca gaggcggtaa cgtgtcgggg gctttggtga tgagctggat gcggcgttcc
    26881 tgtgcttcgt actgcaagag cgagataata tgagagagca gttgtttcgt atcgatgggg
    26941 tgaaggggga ccgaaccggg tcgtgcgaac ttcataaacg tgtccagaat ggccgaaatc
    27001 cggcgcgttt ccgcgttcaa gacgtgcatg tatctatctc tcgtggggga aagtcgctcg
    27061 ccgccgcgca gttcctcttc gaggagatgg aggttcaaat cgatggcact caagggatta
    27121 cgcaactcgt gcgcgacgcc ggctgacagc gtatgaagtc ccgccagctt gtccgccacc
    27181 cgtacgcgcc gctccaggtc cagcagttcc gtgacgtctt tcagcagcac aatcacgccg
    27241 gccgactggc cgtcgtctcc ggtcagatcc gccgtcgcga cgcgtatcgt gcgggtctgc
    27301 tcgccggtcc catagggcat gtcgtgatga tccacatggc ggttggtgcg gagcgctgcg
    27361 tcgaggacga tccggatcat gtctccttct ccaaacactt tgtcatagct ctgctcgagc
    27421 aattcatccg tcgagcgttt caaggtcgcc actgcagagg gattgaccgc cgtgatttgg
    27481 ccgtcccgat tcacggtgac gacgccagtc ggaatgcttt cgagaatgtt ccgggcaagg
    27541 cctttgactt cttcaagggt ccggcgtgtg ctgttgtaat gctgaaaagt gatgacggct
    27601 gcgatcccga tgccgctcag aaggaacaat aaaatagtca tcacaacgag gtcgtagcgg
    27661 gattgccagt ccccgccgag cagttcggtc ggaacgtccc tgccctccgt caatccttga
    27721 accacaaatt gctcatgctc gaaactgaac agtaagagcg cagacaccat cagggcgagg
    27781 ataagcagaa cggtggcgat caaacgcagg gggatgattt gaaagagcgt cgtttcaggg
    27841 gaaggtcgaa acatggtggt tgaccgccga aagccgaata aaacaatgct accacagcgg
    27901 gaatgtgcag tgcacgggac gttccaggcg cccgttcaag gaggcgatcc tctcacgtga
    27961 gtgcgatgct tcggtcagca tactctctca atgccgggtc gttggtcacg aagatcgcag
    28021 cccagggctc ctcttttgaa cagagccttc ggaggaggac ttgccgcagg cttggctcca
    28081 tattgtgcaa ggttccatcg aagatcagca acggcgggcg cattacgatt gcgcgggcca
    28141 cgagaatacg aagtatctgg ctcttcgtct atcggctgcc cccgacatcc gcacaagtgt
    28201 cgaggccgtg aggtattcgg ctcaaacccg ataggtgatg ctgttcgatt gcttcgtcga
    28261 gcccggcctc agatgcgagc ccgctgctca gccggagcag ccactcctcc tctgcgccat
    28321 caagccaacg ccggtgcctc ggcaacggac tcatgtcaaa tctgctgatc tcgttgtcat
    28381 ttcaaggaaa ccgaccgggg aaaggtcctc tcctctgttc acgtgggcgc tgcgccccgc
    28441 accggttgat tcgggatctg caaatattgc agcgtgtg
//
LOCUS       DATIFR010000173         9374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1451614, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000173 DATIFR010000000
VERSION     DATIFR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451614"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..282
                     /locus_tag="VLD60_09980"
     tRNA            207..282
                     /locus_tag="VLD60_09980"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Asn,seq:gtt)
     gene            346..1323
                     /locus_tag="VLD60_09985"
     CDS             346..1323
                     /locus_tag="VLD60_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB45331.1"
                     /translation="MPLSSFQPPCPLRNAHLMTLLSPYWPRGPLLDTIPVESRLFPVA
                     PETSILGYCHWQPRRQSSAVVVLVHGLEGCSESHYMRGIARKAYRAGLNVVRLNQRTC
                     GGTEHLSRTLYNSGLSEDYRTVVEEFARTDRFDRIWLVGYSMGGNLVLKAAGEFAQSE
                     PALAGVIAICPNIDPTQCVIALEQPRNWIYHRHFLMSLKARLRRKAEHLPGKWDLTGL
                     ERIARISEFDDRYTAPDGGYRDSSDYYDRSGARHVLGSIAVPTTIITAQDDPFIPYSM
                     FGTPAIQNNPSIRLIAPHHGGHCGFFQGFSNGEDPYWAENRIVDIVRGG"
     gene            complement(1371..1832)
                     /locus_tag="VLD60_09990"
     CDS             complement(1371..1832)
                     /locus_tag="VLD60_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45332.1"
                     /translation="MMKARKWMMEYGVAMFLAVLFAAVLGHLPLFRETAVGKLHASDL
                     VQFLGYGGALIIAWFGARQLAVEPPDDWKWLVPYRALILPVTTLGMVGLAYGVLLYAC
                     GPFLNKSAKDIYNWTFVIALIAACVWLIVTWVQKCASQVAAMDARRLRRAA"
     gene            complement(1932..3416)
                     /locus_tag="VLD60_09995"
     CDS             complement(1932..3416)
                     /locus_tag="VLD60_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSB45333.1"
                     /translation="MLNRIFGLETEYGLLINQERPDHSPTWFAHKIRDHLFHVQQRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYLDMGHLEYASPECQSLTDVVASDRAGDLLIQQA
                     IQDLGVADNTSLIKNNIDHETDATFGSHENYLVTRRFPFSRRGLSPLVTFLVTRQIFT
                     GSGRVGAANPQDAWIQMDRLIVPRGAMQHAHGPSQIPFQISQRADYIVNDFFEWVQQN
                     RAIVNTRDEPLADPNQYRRIHLLLGDSNMAEYATALKLGTTGLVLQLIEEGRAPGGLD
                     LREPVETLQEISQDQDRQWLVTLQSGATMSAIDVQEQFLAAARQRYEGQDEETDWVLD
                     QWEAVLQDLRGDYTKLVGRVDWASKLWLLESYREAEGAGWDDPMLKSLDLEYHNLHPD
                     RGLYYGLLEEGRVPRLTSDKAIALATDHPPRNTRAFGRGELVRHLLACGPSPEPDRPK
                     PDERFFPSYVINWSIFQLRGQVPFPMPDPFKTYVQEVRAHVAGS"
     gene            complement(3418..4242)
                     /locus_tag="VLD60_10000"
     CDS             complement(3418..4242)
                     /locus_tag="VLD60_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45334.1"
                     /translation="MYEEPYRWVEAVSNRRTYLDEQFKQGSPVVAFAYDGGLLLTTVS
                     KGTPKLYEIYDRIALAGMGHPADLEKLRFSLLEMAHTEGFNRSPSDVTGARLVKYGVA
                     PVIKQAFEEVFKAPFIVKILLAELGQKREKDAFLTINYDGTFEETTAYAVLAATPTIQ
                     KEMTAYLRQEAGPALSLEQAVGVALRTWALGDRAQQQAVEESNQSDETSRSSRAAPLP
                     TDAASLLAHLRSSVGEKMIECALLERNYPGTSKYRALKPDELGRLLPKDLRSPVAE"
     gene            complement(4254..5030)
                     /locus_tag="VLD60_10005"
     CDS             complement(4254..5030)
                     /locus_tag="VLD60_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSB45335.1"
                     /translation="MGMQGDFYQLLKEQGYQLGGSAAGAPGQEMTTATTILAFKYRDG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDPHSVLAIAGVPATAYEMVRILEHSFKYYRRT
                     QLQELSFDGKLRAVSKLLKENVAAALAGTGAVVPVFAGYDVDHGAAKIYFYDILGAEF
                     EGVEYAVSGSGSPTIRGILHYLNTWGDRPLSALSEEEATIQTLRLLTSAAEFDSATGG
                     VNRDANLYPVVKLITQDGLRTVPDSTLKPLFERNVTRVRR"
     gene            complement(5113..5307)
                     /locus_tag="VLD60_10010"
     CDS             complement(5113..5307)
                     /locus_tag="VLD60_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HSB45336.1"
                     /translation="MTYISMPERREGPVDPMPKAQKPSNEGSGPRKPETGSPDKDNLL
                     KRMRKVDPKQAERYRQRTGQ"
     gene            complement(5304..6905)
                     /locus_tag="VLD60_10015"
     CDS             complement(5304..6905)
                     /locus_tag="VLD60_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSB45337.1"
                     /translation="MRTNERAPFRQAVSFDASRFTPHGMMRLFGIETEYGITRDDLEQ
                     VDPVVESMELVRAHLTASFERRWDYAGEDPHEDARGFRVSGLQQDKEEDEFAKVDAHR
                     PFSFHEMKSDLVLPNGARFYNDHTHPEYSTPECRTLKDLLAHDRAGERIVQRAAERRN
                     RALGGPHVQLYKNNTDFHGHSYGCHDNYLVPRAIPFSSLMTGLLPFLVSRQVIAGAGK
                     MGVEAQESGFVPGRYQLSQRADFMETDMSVDTMHNRPILNTRDEPHADRTKYRRLHLI
                     IGDANMCEYATALKIGTTQLVLQLIARQAAPAIELDHPVTAVKQVSRDQDLKATVRRK
                     NGRSITGLEIQEQYYTAAEKHLAGSDPETDWILREWKATLQWLTRDRGQLVGWLDWVT
                     KLWLLETFVREERLGWDDPWLASLDLEYHNVNPEQGLYLGLEADGKVWRMTTDADVDA
                     AMAEGPRDTRGGLRGLCVRRFPDQIKSMQWERIQFSGGLLPRTLEMGDLFEPSEVKAC
                     AAAFETAASPMDALKAWNNGKESQS"
     gene            complement(6938..8689)
                     /locus_tag="VLD60_10020"
     CDS             complement(6938..8689)
                     /locus_tag="VLD60_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB45338.1"
                     /translation="MSDRRPTDHPHDEPQGKREGPTGASTKTGSDPLELIEECLASFS
                     DTDPRQKILYKLRHAVMLSQAAHHQREAEFKKVSEVVAKLTAPANRIGTLLELPGEGL
                     ARIVVGGAEYYANVDPRVSAADLKIGTQILVNEAYAVIKTLGYDSNGPVLKVAETLPD
                     GRLRFEQEMGRQVLVLQRSSDLIGVDIKSGEEVRIDPSLHVAIERMEDRKARKHLLDE
                     TPRVTWEQIGGQQEAIASIRKAIEYPLLHRKTFEQFKFTQPKGFLLYGPPGCGKTLIG
                     QAAAASLSKLVSESSQADAAATADKPPVTGGTFLHVKGPEILNMWLGESERMVRDLFA
                     QARARRKEGALPFIFIDEAESILGTRRATRSFNISSTLVPMFCSEMDGIESLRDVVII
                     LASNRPDLIDPAVLRPGRIDRKIKVSRPNRESAAEILKVYLTNELPLDEALVAERGGD
                     KSKAFAWLVDEVINAIFKRTDENRLLSVRLRSGQNKVLYRNELVSGAILSSIVQRAKE
                     KAIDRTIQSGKPAGLVAEDLLDAVGEEFREGEVLPPDDAAEEWLKLLDHHPEQVVGIS
                     SFRRGRQTEERLINQII"
     gene            complement(8755..>9374)
                     /locus_tag="VLD60_10025"
     CDS             complement(8755..>9374)
                     /locus_tag="VLD60_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB45339.1"
                     /translation="QKVLAIGNPFGLDHTLTTGVVSALGRTIRSLTNRTIEGVIQTDA
                     AINPGNSGGPLLDSAGRLIGVNTQIVSPSGAYAGIGFAVPVDMVNRIVPELVKFGKLI
                     RPGLGVSLVPDAMARRWGIKGLIIGKVSRGGAADRAGLKGARETVTGRVELGDIIVAV
                     AGKPVVTIDDLMDVMEKHKVGDQVTVEILRANRRQSMAVTLQAVN"
BASE COUNT         1924 a         2977 c         2484 g         1989 t
ORIGIN      
        1 ggcggacacg atgcgcgata gcggcaatct ctatttgacc tgggccaagc actacgcggc
       61 aatggcggca ggcaacaccg acgcctcctc ggacgaagac gaaactgaag acttcgacgt
      121 gtagcacgga gcgtgaaaaa actctcccct tcccccgatc acttttgcta caatgagtac
      181 tttgctgagt tcagcacctc tagcgttccc cggtagctca gttggtagag cagccggctg
      241 ttaaccggct ggtcgcaggt tcgagtcctg cccggggagc catatccata aaaagggcac
      301 cgttcgcggt aggaacggcg ccctttttct tttaggatgc aacatatgcc gctgtcttca
      361 tttcagccgc cctgcccact tcgcaatgcg catctgatga cattgctctc tccttattgg
      421 cctcgtggtc ctctactcga caccattcca gtcgagtcac ggctgtttcc tgtggcaccg
      481 gagaccagca tcctcggcta ctgccattgg cagccgcgtc gccagtcttc tgcggttgtg
      541 gttctcgtcc acggccttga gggttgcagc gaatcccact acatgcgtgg gattgcgcgg
      601 aaagcctatc gtgcgggact gaatgtcgtc aggctgaatc agcggacctg cggtgggacg
      661 gaacatctca gccgaacact ctacaacagc ggactcagcg aagattaccg gacggtcgtc
      721 gaagagttcg cacgcacgga tcggttcgac agaatctggc tggtaggcta ttccatgggc
      781 ggcaatctgg tgctgaaagc agcaggggaa ttcgctcaaa gcgaaccggc gctggctggc
      841 gtcattgcca tctgtccgaa tatcgatccg acgcagtgcg tcatcgcctt ggaacagccg
      901 cgcaattgga tctaccaccg gcacttcttg atgagtctga aagctcgctt acggcgcaaa
      961 gccgaacatc ttcctgggaa atgggatctc acgggtctcg agcgcatcgc caggattagc
     1021 gagttcgatg atcgctacac cgcccccgac ggaggatacc gagatagttc cgattattac
     1081 gaccgctccg gcgcccggca tgtcctcgga tcgatcgccg tcccgacgac aatcatcacc
     1141 gctcaggatg acccgttcat cccctattcc atgtttggga ctcccgcaat tcagaacaat
     1201 ccatccattc ggctcattgc accacaccat ggaggtcatt gcggattttt tcaagggttt
     1261 tcaaacggag aagaccctta ctgggccgaa aaccggatcg tggatattgt acggggaggt
     1321 tgaagagaag agcggctgcg ccgaaccagg cgcagccgga gcaggatcaa ctaggcagcc
     1381 cttcgcaatc tacgagcatc catcgccgca acttgtgagg cgcacttctg cacccacgtt
     1441 acaataagcc agacacatgc tgcgatgagg gcgatgacga acgtccaatt gtatatgtcc
     1501 tttgcggact tattcagaaa gggtccgcag gcatacagca agacgccata ggccagtccc
     1561 accattccca gcgttgtcac tggaaggatc aaggcccgat agggcaccag ccacttccaa
     1621 tcatctggcg gttcaaccgc caactggcgc gccccaaacc aggcaatgat caacgctcct
     1681 ccgtatccaa ggaactgaac caagtctgac gcatgaagtt ttccgacggc cgtttcacgg
     1741 aacagcggta agtgtccgag caccgcagca aagagaaccg ccagaaacat cgcgaccccg
     1801 tattccatca tccatttgcg tgctttcatc atgaacctcc cctcagcctg taaaagggct
     1861 cttgtcgagc cacgtcaagc atagcacctg agtatttcgc tcagatgatc aatgagttag
     1921 ggagggctat ttcaggatcc tgcgacgtgg gcgcggactt cctgcacgta ggttttgaaa
     1981 gggtccggca tgggaaaggg gacctggccg cgcagctgga aaatggacca gttgatgacg
     2041 taggaaggga agaaacgctc gtccggtttc ggacgatccg gttcaggaga cggtccgcag
     2101 gccagcaagt gacgaacgag ttctcctcgt ccgaacgctc gggtgttgcg aggtgggtga
     2161 tccgtggcca gcgcaatggc cttatcggac gtcagccgtg gcacacgtcc ttcttccaac
     2221 aacccataat agagcccacg gtccggatgg aggttgtgat attcgagatc caggctcttg
     2281 agcataggat cgtcccaacc tgcgccttcc gcctcacggt acgattcgag taaccacaac
     2341 ttcgacgccc aatccacccg tccgaccagc ttggtgtagt cgccccgcaa atcctggaga
     2401 acggcctccc actgatccag cacccaatcg gtctcttcat cctgcccctc ataccgttgc
     2461 ctggctgcgg ccagaaactg ctcctgaaca tcaatcgccg acatcgtcgc gcccgactgc
     2521 aacgtgacaa gccattgccg atcctgatcc tgggagatct cttgaagcgt ttcgactggt
     2581 tcgcggagat ccaacccgcc cggagcccga ccctcctcga tcaactggag gaccaagccc
     2641 gtcgttccaa gcttcaacgc cgtcgcatat tcagccatat tggaatcgcc cagcagcagg
     2701 tggatgcggc gatactggtt cggatccgcc agcggctcgt cgcgcgtatt gacgatcgcc
     2761 cggttctgct gtacccactc aaagaaatcg ttcacgatat agtctgcacg ctgtgaaatc
     2821 tgaaatggga tctgcgacgg cccatgcgca tgctgcatag ccccgcgcgg cacgatcaat
     2881 cgatccatct gaatccaggc atcttgcgga ttggcggccc cgacgcggcc cgatccggtg
     2941 aaaatctgac gcgtgaccag aaacgtgacc agaggactga gtccgcgccg gctgaatgga
     3001 aagcgccggg tcacgagata gttttcgtgt gaaccgaagg tcgcgtcggt ctcatgatcg
     3061 atattgttct tgatcaacga ggtgttgtcg gcaacaccga ggtcttgaat ggcctgttgg
     3121 atcaacagat cgccggcgcg gtcggaggcc actacatcgg tcaatgactg gcactccggc
     3181 gaggcatatt cgagatgccc catgtcgaga tagatgcggc cggcattggt cagaaacccg
     3241 ccgttaccag ggggctcatc atggccgcga tggtgcagat cgaggacacc acgctgctgc
     3301 acatggaaca gatgatcgcg gattttgtgg gcaaaccagg taggcgaatg atccggccgc
     3361 tcctgattga tcaagaggcc gtattccgtt tcgagtccga agatgcggtt cagcatctca
     3421 ctctgccacc ggagatcgaa gatccttggg aaggagccgc cccaattcgt cgggtttcaa
     3481 cgctcggtat ttcgaagtcc ccggatagtt ccgttccaac aacgcgcact cgatcatttt
     3541 ttctcccacg ctcgaacgca aatgagccag cagtgatgcc gcatcggtcg gaagcggcgc
     3601 ggccctggac gatctgctcg tctcatccga ttgattcgac tcctccaccg cctgttgctg
     3661 cgcccgatcc cccagcgccc aggttcggag tgcaacccct acagcctgct caagcgagag
     3721 tgccggccca gcctcttgtc gaaggtaggc cgtcatctcc ttctgaatcg tcggcgtagc
     3781 ggcaagcacc gcataggcag ttgtttcttc aaacgtgccg tcgtagttga tcgtcaggaa
     3841 ggcatccttc tcgcgcttct gccccaattc tgccagcaga atcttgacga tgaacggcgc
     3901 cttgaacact tcttcaaagg cctgtttgat caccggcgca acgccgtatt tcaccaagcg
     3961 cgcgccggta acatccgacg gtgagcggtt aaacccttcc gtatgtgcca tttccaacaa
     4021 gctgaaccgg agtttctcca ggtcggctgg atgtcccatg cccgccaagg cgatgcgatc
     4081 atagatctcg tagagcttcg gcgtcccctt gctgacagtc gtcagcagca ggcctccgtc
     4141 ataagcaaac gcgacaacag ggctcccctg cttgaactgt tcgtcgagat aggtccgccg
     4201 attgctgacc gcttcgaccc accgataggg ttcttcatac atggctggtt cccttatcta
     4261 cggactcggg tcacgttccg ttcaaacagc ggcttcagcg tggaatcggg aactgtccgc
     4321 aacccgtcct gtgtgatcaa tttcaccacg ggataaagat tggcgtcacg attcaccccg
     4381 ccggtcgccg aatcgaattc cgccgcactc gtcaacagcc ggagcgtctg gatcgtcgct
     4441 tcttcttcag acaaggcact cagcggccgg tcgccccagg tattcagata gtgcaggatg
     4501 ccgcggatgg tcggcgaacc ggaccccgac acggcatact ccacgccttc gaactcggca
     4561 ccaagaatgt catagaaata tatcttggcc gctccgtggt cgacatcata ccccgcaaaa
     4621 accgggacaa ccgcgccggt tcccgccaaa gccgccgcca cattttcctt caagagcttg
     4681 gaaaccgcgc gcagcttgcc gtcgaaactc agttcttgga gttgcgtccg ccgataatac
     4741 ttaaaagaat gctccaagat gcgcaccatt tcataggctg tcgccggcac gccggcgatc
     4801 gcgagcacgc tgtgcggatc gatctcgagc accttgtccg tccgatcgta catcaccata
     4861 ttgccggccg tcgcccggcg gtcaccggcc actaagacgc cgtcacgata cttaaacgcc
     4921 aggatcgtcg tggccgtggt catctcctgc ccaggggctc cggcggcaga gccgccgagc
     4981 tgatagccct gctctttgag tagctgatag aaatcgcctt gcatgcccat gtgtcgctct
     5041 gctccgttag gggtaaggag tgaggagtaa agggcaggag ctggcttccc tcaccttacc
     5101 cctcactgtc gtttattgtc ctgttcgctg ccgatagcgc tccgcttgtt tcggatcgac
     5161 cttgcgcatc cgtttcagga gattgtcctt gtccggcgag ccggtctctg gtttccgagg
     5221 cccactgcct tcattggatg gcttctgtgc cttgggcatc ggatcgaccg gtccctcgcg
     5281 tcgttccggc attgaaatgt atgtcatgat tgcgattcct ttccattatt ccacgctttc
     5341 agtgcgtcca tcggagacgc ggcggtctca aaagccgccg cgcaggcctt cacctccgat
     5401 ggctcgaaga gatcgcccat ctccagcgtt cgagggagca gccccccgct aaattgaatc
     5461 cgttcccatt gcattgactt aatctgatca ggaaaccggc gcacacaaag acctcgcaag
     5521 ccaccgcgcg tatcacgcgg gccttccgcc atcgccgcat cgacatcagc atccgttgtc
     5581 atccgccaca ccttgccatc agcttccaga cccaggtaca gcccctgttc aggattcaca
     5641 ttatgatatt ccaaatccaa actggcgagc caggggtcgt cccaccccaa gcgctcttcc
     5701 cgcacgaagg tttcgaggag ccacaatttg gtcacccaat ccagccaccc gaccaactgc
     5761 cctcgatcgc gggttagcca ctggagcgta gccttccact cgcgcaggat ccaatcggtc
     5821 tcgggatcag atccggcaag gtgcttttcc gctgccgtgt agtactgttc ctgaatctcc
     5881 agcccggtga tcgatcgccc gtttttccgg cgcacggtcg ctttgagatc ctgatcgcgc
     5941 gagacctgct tcaccgccgt gacaggatgg tccagctcaa tcgccggcgc cgcctgccgt
     6001 gcgatcaact gcaacactaa ctgggtcgtc ccgatcttca aggctgtcgc gtactcgcac
     6061 atgttggcat cgccgatgat cagatgcagc cgacgatatt tcgtgcgatc ggcatgcggc
     6121 tcgtctctcg tattaaggat cggccgatta tgcatcgtgt cgacgctcat gtccgtctcc
     6181 atgaagtcgg cccgctggga gagctggtac cgccctggca cgaatccgga ctcctgcgcc
     6241 tccacgccca tcttgcccgc gccggcaatc acctgacggc tgacgagaaa cggaagcagg
     6301 cctgtcatca gcgatgaaaa agggatcgca cgcggcacca aataattgtc atgacagccg
     6361 tagctgtgcc cgtgaaaatc cgtgttgttc ttatagagct gcacatgcgg cccgcctagc
     6421 gcacgattgc ggcgctcggc ggcgcgctgc acgatccgct cccctgcccg gtcgtgcgcc
     6481 agcaggtctt tcagcgtacg gcattccggc gtcgaatact ccggatgcgt atggtcgttg
     6541 tagaagcgcg ccccgttcgg gagcacaaga tcgcttttca tctcgtgaaa cgaaaagggc
     6601 cgatgggcat cgaccttggc aaactcgtct tcttctttgt cttgttgaag cccggagacc
     6661 cggaaccccc gcgcatcctc atggggatct tcgccggcat aatcccaacg ccgctcgaac
     6721 gacgcggtca ggtgggctcg caccaattcc attgactcga cgacgggatc aacctgttca
     6781 agatcgtcgc gcgtaatccc gtactccgtt tcaatgccaa acaaccgcat catgccgtga
     6841 ggcgtgaaac gtgaggcgtc aaaggaaacg gcctgcctga acggagccct ttcatttgtt
     6901 ctcacccctc atgccttact cctcacccct tacgtcttca aataatttga ttgatcagcc
     6961 gctcttctgt ttgccgcccg cgccggaatg aggaaatgcc gaccacctgt tcaggatggt
     7021 ggtccagcag cttcagccac tcttcagccg catcgtccgg cggcagcacc tcgccctcac
     7081 ggaattcctc gcccaccgca tcaagcagat cctccgccac gagccccgcc ggctttcccg
     7141 attgaatagt tcgatcaatg gccttttcct tcgcacgttg aacaatcgat gacagaatcg
     7201 ccccgctgac caactcgttc cggtagagca ccttgttctg gccgctccgc agccggaccg
     7261 acagcaaccg gttctcatcc gtccgtttaa agatggcatt gatgacctca tccaccagcc
     7321 aggcaaatgc cttcgacttg tctccgcctc gttcagctac cagcgcctca tcgaggggaa
     7381 gctcgttcgt caaatagact ttcaagatct ccgcggcgga ctctctgttg ggacggctaa
     7441 ccttgatttt tcgatcgatc cggcccggcc gtagcacagc agggtcgatc aggtccggcc
     7501 gattcgaagc caggatgatc accacatccc gcagcgattc gatgccgtcc atctcggaac
     7561 agaacatcgg gaccagcgtg cttgaaatat tgaaagatct ggtggcgcgc cgcgtgccga
     7621 gaatcgattc ggcttcgtcg ataaagatga atggcaacgc tccttctttc cggcgtgcgc
     7681 gtgcctgcgc aaacagatcg cgaaccatcc gctccgactc acccagccac atgttcagaa
     7741 tctcaggccc tttgacatgg aggaacgttc cccccgtcac cggcggcttg tcggctgtcg
     7801 cagcagcatc cgcttggctc gactcgctca cgagtttcga gaggcttgcc gccgcagcct
     7861 ggccgatgag ggttttgccg caaccgggcg ggccatagag gagaaatcct ttgggctggg
     7921 taaatttgaa ctgctcgaac gtcttcctat ggagcagcgg atattcaatg gccttacgga
     7981 tcgaggcgat ggcctcctgc tgaccgccga tctgctccca cgtgaccctc ggcgtttcat
     8041 ccaagaggtg cttgcgcgcc ttccggtcct ccattcgctc gatcgccaca tgaaggctgg
     8101 gatcaatgcg aacctcctca cccgacttga tgtcgacacc gatcaaatca ctcgaccgtt
     8161 gcaacacgag cacctgccgg cccatttcct gctcaaatcg caagcggcca tcaggcaatg
     8221 tttccgccac cttcagcacc gggccgttgg aatcgtagcc gagcgtcttg atcacggcgt
     8281 aggcttcgtt caccaaaatc tgcgtgccga tcttgagatc ggccgccgaa acacgcggat
     8341 cgacgttcgc gtaatactcc gctccgccca caacaatgcg ggccaatccc tcaccgggca
     8401 gttcgagcaa cgtgccgatc cgattggccg gcgccgtcag cttggccacc acctcactga
     8461 ctttcttgaa ttccgcttcc cgctgatgat gagccgcctg cgacagcatc acggcatggc
     8521 ggagcttgta gagaatcttt tggcgtgggt ccgtatccga aaacgaagcg aggcattcct
     8581 cgatcaactc gagcgggtcg gaacccgtct tggtggaagc gcccgtcggc ccttccctct
     8641 taccctgtgg ttcgtcgtgc gggtgatcgg tcggtcggcg atcgctcatc agaaatcctc
     8701 cgctcaggac agaatgggtg atcctaccac accgaccgaa cggattaccg ccagctaatt
     8761 cacggcttgc agggtcacag ccatgctctg tcgtcgattg gcacggagga tctccactgt
     8821 gacttgatcg ccgactttat gcttctccat cacgtccatt aagtcgtcaa tcgtcacgac
     8881 cggtttgccg gcgaccgcca cgatgatatc gccaagttcc actcggccgg tcactgtttc
     8941 acgtgcacct ttgagaccgg cccgatcggc tgcgcctcct cgcgaaacct tcccaataat
     9001 caaccctttg atcccccatc gcctggccat cgcatcgggc accaacgaca cgcccagccc
     9061 cggccgaatc agcttcccaa acttcaccaa ttcgggaacg atccgattca ccatatcgac
     9121 cggcacggca aatccaatac cggcataggc gccactgggg ctcacgattt gcgtgttcac
     9181 gccgatcaaa cgccccgcgc tgtcgagcaa cggcccgccg ctgttgccgg gattgatcgc
     9241 cgcatcggtt tgaatcacac cctcgatggt tcgattcgtc agcgacctga tcgtccgccc
     9301 cagggcgctc acgacaccgg tcgtcagcgt atgatccagt ccgaacggat tcccgatcgc
     9361 caagaccttt tggc
//
LOCUS       DATIFR010000174         4349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_495560, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000174 DATIFR010000000
VERSION     DATIFR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495560"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /gene="rpoC"
                     /locus_tag="VLD60_10030"
     CDS             complement(<1..2159)
                     /gene="rpoC"
                     /locus_tag="VLD60_10030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSB45340.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVCVDPGSTDLSEKELVPEDKLRTLLSEFGSSGFKV
                     GIGAEAIRDLLRKIDINSLWDELQVKAKASTSAAMKKKYAKRLKVLEAFRKSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERLGARGEGKI
                     FGSPEEVRIAFDAREVEEHARIKVRLAGALVQTTVGRVLLSEILPAGMPFANANKLMT
                     KKEMTKLIDSVYRQTGHRDTVEFLDKIKDIGFTYATRAGVSICIDNMHIPTKKEDFIG
                     KAQREVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPGKAESF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIET"
     gene            complement(2263..>4349)
                     /gene="rpoB"
                     /locus_tag="VLD60_10035"
     CDS             complement(2263..>4349)
                     /gene="rpoB"
                     /locus_tag="VLD60_10035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSB45341.1"
                     /translation="QANSKVDGAGRLVSETVSCRHGGDFVMATPDKIEYMDVSPKQVV
                     SVATALVPFLEHDDANRALMGSNMQRQAVPLVTSESPLVGTGMEAVVARDSGYVIQAR
                     RAGVVESVDATRIVVRADSKDGKRGKDSGLDVYDLIKFQRSNQNTCITQTPVVRIGQP
                     VKKGQVLGDGPAIDHGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIE
                     EFEVEARDTKLGKEDVTRDIPNVGEEALRNLDESGIIRIGAEVKPGDILVGKVTPKGE
                     TQLTPEEKLLRAIFGEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIET
                     DDQMKLQRDHHEELRIIEEEKTKKIRKLLLGKVVGRDLMDPDTGDVILKKKGKLTAEI
                     LKRLPDDTVRHIILSDPDEQKELEDVERRAKEQIEILQTLYDEKVGRLKRGDELPPGV
                     IKLVKVYIAMKRKIQVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVP
                     SRMNVGQILETHLGWAAKALGIKVASPVFDGASEKEIKDLLKKAKLPPSGQSQLVDGK
                     TGEPFGSPVTVGYMYVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEME
                     VWALQAYGAASTLQEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQS
                     LGLDVELVKTQD"
BASE COUNT          809 a         1409 c         1145 g          986 t
ORIGIN      
        1 gtttcgatga tttcgccgga tggtttggcc atcaggccgc gcatgccgcc caactgccgg
       61 atctgctgcg agctgccgcg cgcaccggag tcggccatca tgaagatcgg attgaaagat
      121 tccgccttgc ccggatctcc gccggcaccg agttccttca tcatctcatt ggcgacctgc
      181 tcggtcacat gggcccagat gtcgatgacc ttgttatacc gctcgccgtt tgtgatgaga
      241 ccctcggagt actgcttctc gatttcgttg acttcgcgct gcgctttccc gatgaagtct
      301 tccttcttgg tcgggatgtg catgttatcg atgcagatcg acacacccgc ccgcgtcgcg
      361 taggtaaacc cgatgtcctt gatcttgtcg aggaactcga cggtgtcgcg atggccggtc
      421 tgccgataga ccgaatcgat cagcttcgtc atttccttct tggtcatgag tttgttcgcg
      481 ttggcgaacg gcatgcccgc gggaaggatt tctgatagca acacgcgacc gaccgtggtt
      541 tgcaccaatg ctccggccag gcgcaccttg atccgcgcat gctcttccac ttcgcgcgca
      601 tcaaacgcga ttcgcacttc ctccggcgaa ccgaagatct ttccctcacc tctcgcaccc
      661 agccgctcct tcgtgagcca atagcagccg agcaccatgt cttgcgacgg gacggcgatc
      721 ggcttgccgt tggcgggcga cagaatgtta ttgatggaca tcatcaacac ccgcgcctcg
      781 acttgcgcct cgacggacaa cggcacgtgg accgccatct ggtctccgtc gaagtccgcg
      841 ttgaatgcgg cgcacacgag cggatgcaac cggatcgcct tcccttcgac cagcaccgga
      901 tcaaacgctt gaataccaag tcggtgaagc gtcggggcgc gattcagcaa caccggatgc
      961 tcgcgaatca cttcatcgag cacatcccac acctcaggcc gttctttctc aaccaggcgc
     1021 ttggcgctct tgatcgtcgt ggccgctccc cgctcttcca gcttgtgaaa gataaacggc
     1081 ttgaatagtt ccagcgccat cttcttgggc aacccgcact gatgcagacg caactctgga
     1141 ccgacgacga tgaccgtacg gccggagtaa tccacgcgtt tcccgaggag attttgacgg
     1201 aaccgcccct gtttcccctt cagcatgtcg ctcagggact tgagcggacg cttgttgggt
     1261 ccacggatcg cacggcctcg ccggccgttg tcaaagagcg cgtcgactgc ctcttggagc
     1321 atccgcatct cgttccgaat gatgaccccc ggcgccttca attccatcaa ccgcttcaac
     1381 cggttattcc ggttgatgac gcgccgatag agatcgttca agtcggaagt cgcaaagcgg
     1441 ccgccatcaa ggggaaccag aggacgcaat tccggcggaa gcaccgggat cacatccatg
     1501 atcatccatt ccggcttgtt tccggatttg cgaaacgcct ccaatacttt gagccgcttg
     1561 gcatatttct tcttcatggc ggccgaggtc gacgccttcg cctttacctg caattcatcc
     1621 cacaaggaat tgatatcgat cttccgcagc aagtcacgga tggcctctgc gccgattccc
     1681 accttaaatc cgctggaacc gaactcggac aggagggtgc gcagtttatc ctcgggcacc
     1741 aactcctttt cagacagatc cgtggaaccc ggatccacgc agacgtagct ctcgaagtag
     1801 aggatcttct ccaactgctt gaggctcatg tcgagcaagg tccctatgcg actgggcacc
     1861 ccctttagga accagatgtg cgcgaccgga gcggccaatt cgatatgccc catgcgctcg
     1921 cgccgcactt tcgactgaat cacttcgacg ccgcacttgt cgcagacgat gccgcggtgc
     1981 ttcatgcgct tatatttgcc gcaattacac tcccagtcct tgatcggacc gaaaatcttc
     2041 gcgcagaaca atccgtcttt ttccggtttg aacgaccgat agttgatcgt ctccggcttc
     2101 ttcacttcgc catacgacca ggaccggatt ttctcgggcg acgcaatgcg gatgcgcatc
     2161 gaatcgaacg acaccgaatc ccgcggtttt tcaaacaatg tatatacgcc ttccaaggta
     2221 gtgacctcct ctgataccgg ctttcaaccg gcacacaagt ggttagtctt gcgtcttgac
     2281 caactcaacg tcgagcccca agctctgcag ctctttgacc aagacgttaa acgactcggg
     2341 caaccccggt tccaggaagg gttcgccttt cacgattgct tcatacatgc gcgaccggcc
     2401 cggcacgtca tcggacttga ccgtgaggaa ctcctgcagc gtcgaggccg caccgtaggc
     2461 ctgcaacgcc cagacttcca tttctcccag gcgctggccg ccgaattgag ccttcccgcc
     2521 cagcggttgc tgtgtcacaa gcgaatacgg accgatggac cgtgcatgga tcttgtcatc
     2581 caccaggtgg tggagcttga gcacatacat atagccgacg gtgaccgggc tgccgaaggg
     2641 ctcacccgtc ttgccatcga ccagctggct ctgcccgctc ggcggcaatt tcgccttctt
     2701 gagcagatcc ttgatttcct tctccgaggc gccgtcgaac accgggctgg ccaccttgat
     2761 ccccagggct ttggccgccc agccgaggtg ggtttccaaa atctggccca cgttcatacg
     2821 ggacggcacg cccagcggat tcagcacgat ctcgaccggc gtcccatccg gcaagtacgg
     2881 catgtcctct tccggcaaca cccgggagac cacgccttta ttgccgtggc gaccggccat
     2941 tttatcgccg acctgaatct tgcgcttcat ggcgatgtag accttgacca acttgatcac
     3001 gccggggggc aattcgtcgc cccgcttcaa gcgaccgact ttctcgtcat agagcgtctg
     3061 gagaatttcg atctgctcct tcgcgcgtcg ctcgacgtct tccagttctt tctgttcatc
     3121 cggatcgctg agaatgatgt ggcggaccgt gtcatcgggc agccgcttga gaatctccgc
     3181 cgtcagcttg cccttcttct tcaagatcac gtcgcccgtg tccggatcca tcagatcgcg
     3241 gccgaccacc ttgccgagca ggagcttccg gatcttcttc gtcttttctt cctcgatgat
     3301 ccgcagctct tcgtggtgat cacgctgcag cttcatctga tcatcggtct cgatgctctt
     3361 cgaccgctca tccttatcga ggcctttgcg cgagaagatc ttgacgtcga ccacgatgcc
     3421 ttcgactcca ggaggcacgg tcaacgaggt atccttcacg tcgccagcct tctcaccgaa
     3481 gatcgcccgc agcaattttt cttccggcgt gagctgggtt tcgcccttgg gcgtcacctt
     3541 gccgaccaaa atgtctccgg gctttacctc ggcgccgatg cggatgatgc cgctctcatc
     3601 gagattccgg agcgcttctt ctccgacgtt cggaatatcg cgagtgacgt cttctttgcc
     3661 gagctttgtg tcgcgcgctt ccacctcgaa ctcttcgata tggatcgatg tgaacgcgtc
     3721 ttcgcgcacc agcttttcgc tgaggagtat ggcgtcttcg aagttgtatc cgccccatgg
     3781 cataaaggcc acgagcacgt tctttcccag ggcaagctcg ccatggtcga tcgccggccc
     3841 gtcgccgagg acttgcccct tcttcactgg ttgaccgatc cgaaccaccg gggtctgggt
     3901 gatgcaggta ttttgattcg accgctggaa cttgatcaga tcgtagacgt ccaacccgga
     3961 atcttttccc ctctttccat ccttcgaatc ggcacgcacc acgatgcgag tggcatcgac
     4021 gctttcgacg acgccggccc gacgggcttg aatgacgtaa cccgaatcgc gcgccacaac
     4081 cgcttccatc ccggtgccga ccaagggcga ctcggacgtc accaggggaa ccgcctgccg
     4141 ctgcatgttg gaccccatga gagcgcggtt ggcgtcgtca tgctccagga acggcaccag
     4201 ggccgtcgcc acgctgacca cctgcttcgg ggacacgtcc atgtattcga tcttgtccgg
     4261 cgtggccatg acgaagtcgc cgccatgccg acacgagacg gtctcagaaa ccagccggcc
     4321 tgccccgtcc actttggagt tggcctggg
//
LOCUS       DATIFR010000175        19340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_110889, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000175 DATIFR010000000
VERSION     DATIFR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19340
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110889"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VLD60_10040"
     CDS             <1..412
                     /locus_tag="VLD60_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45342.1"
                     /translation="KIRNRQDKPVETVVDLLFESEVLLEAQSLDREREVHQHIGDFTL
                     FMAGLFPEYLRRLKTASVIYHKDFLVDYVKTGKRSYGIVAQMADGPSPDEPPLFKKLS
                     DNFELCVTGLGFVRSDLDRMRDPAFRQAQDLLLN"
     gene            409..1296
                     /locus_tag="VLD60_10045"
     CDS             409..1296
                     /locus_tag="VLD60_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HSB45343.1"
                     /translation="MSRSRPLILAHGGAGRRSMTKRQASCLAEALTTGYAVLQRGGSA
                     VEAVECTIGLLEASGFFNAGRGSNRQLDGVQRMDASIMEGARLKAGAVASIEGIVHPI
                     TAARLVMEETDHVLLVGRPAGRLAQHFKLERHHSGHAPRQCAYDKIFDRQKHSRADKH
                     GTVGAVALDESGTVAAGASTGGIQSMLPGRVGDTPLIGCGVYADNRSGAVSMTGLGEG
                     IIRLAIAKTICERLAAGKSPIMAARQVLRQLVSRINGAAGTLVVSPDGRFAIHHVTPH
                     MAAGWWKGSGRPMVKGRFA"
     gene            1293..1733
                     /locus_tag="VLD60_10050"
     CDS             1293..1733
                     /locus_tag="VLD60_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB45344.1"
                     /translation="MRGAIFHLAFPTHDLVAAKQFYVDGLGCTLGRESSRAVTFGLAG
                     HQLVAHLVDESPPVQQGIYPRHFGLVFLSKVPWQELVERAKANRLQFYQQPRVRFPGT
                     RLEHHTFFLQDPSQNLLEFKHYTHESAIFGEHDSRMVGDTSDPD"
     gene            complement(1737..3680)
                     /locus_tag="VLD60_10055"
     CDS             complement(1737..3680)
                     /locus_tag="VLD60_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB45345.1"
                     /translation="MTTDEVVITATRTVEPVSQSASAVTVLTREQIERSPYAGGHQLD
                     DLLRSVPGVQPATLSSRYNHPTAQSITLNGLGVRRTLVLLDGIPLNDGFGGWINWGLI
                     PNDIDRVEIVPGGTSNLYGTWAMGGVVHVITKTPPEGMGFKLDSQAGTLDTYQQALSA
                     RYGTDRFRFSLGYRWFHTNGFIPVPAYQRGPVDRTIDSRHQLFNGRVAWTPSGATTID
                     LSGSLFREDRNFGTNLNLASRTIGNVALGIRSDQGQAGLWESKLFGQWQTFRNVTSQI
                     TPAPLLRLAEFQNVIQTIPSNDFGGSLQWTGSLWGPDRLVLGTDARTIIAQTTDDLFS
                     SSGPTGQTLARGQQIGWGLFGEWILPATGRLTVIPSVRADWWKNFNARGASTTTTTAF
                     EDNTVSVVNPKLSVNYVLTDRVRAGASVYQGFRAPTINELYRGFAFAGFSFLPNDQLR
                     PERLTGGEVKLEGELFDDRALRWRLSVHRDEVKDQILFVSQGPLAAQRQNVGKALSTG
                     GALDLSYRVSPLLSLTFGYAYVDSTITSFPGAQDREGKRIPNVSRNQLSVGMTGGRAD
                     WAEVTIMARYLSRQFTDDFNTQPVADFVVLDASLQHQLRKGLRLVLNGENLTDRHYIA
                     TQTGPVKTLGSPLLVMGGIRAEY"
     gene            complement(3796..4527)
                     /gene="mtgA"
                     /locus_tag="VLD60_10060"
     CDS             complement(3796..4527)
                     /gene="mtgA"
                     /locus_tag="VLD60_10060"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HSB45346.1"
                     /translation="MRKSTKQRRRLTLILFLCIALPIAALLFIWLITMPDIPALQKVN
                     PEMTALIEAREYQARIKGQSIGRHWIWVPLSRIAPDLRRAVVAAEDASFFTHEGFDWE
                     GMKDAALYDLEKGELKRGGSTITQQLAKNLYLSAERSIFRKAREALITRSMEHHLTKD
                     RILELYLNVAEWGNGVYGAEAAARHHFKKPARELTTDEAAWLAAILPAPRRYDPLHKT
                     AALTRRYDRILRRMNQLGRHDIRPD"
     gene            4640..5242
                     /gene="yihA"
                     /locus_tag="VLD60_10065"
     CDS             4640..5242
                     /gene="yihA"
                     /locus_tag="VLD60_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HSB45347.1"
                     /translation="MKILTAEFIRSCVGPEQFPRGTVPEIAFVGRSNVGKSSLINSLL
                     QRKGLAKVSRTPGKTRAVNLFNVATDDPGLSRFVLADLPGYGYAKVSKTERAQWAPLI
                     EQYLSQREQIRAIVMLVESRVLGAHDGETIAWLRSIGLEPLVVATKVDKLKPSERVSA
                     LRRLQEGLGVTDSRTFMVYSSVTGEGRERLWTALKECCRT"
     gene            complement(5166..6218)
                     /locus_tag="VLD60_10070"
     CDS             complement(5166..6218)
                     /locus_tag="VLD60_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSB45348.1"
                     /translation="MPIHTELRKAMTRSRITSIAFASLATATLALTLFAGVPDLRAAK
                     IEDRIVAIVNSDLIMWSEMKRELSPEQERIEKQYRGEELLRKLKAAESMALTTMIERR
                     LQIQEAKVRGIDVSDQEVKQAIKQLNQQGEKLDDKDPAGIKSVREQLILLKVVDREVR
                     SGVMVADPEMRRYYQEHRDRFALPEEYTLSQIFLKPRSPDDLADAKDKAREVMALLKQ
                     GESFEDLALRYSDGPNASRGGKLGLVRQGELLPAIERGVASLVPGGISEPLQSPEGLH
                     IVRVDDKKPKVFRPYEEVKQEIQVLVFQQKSEDMFQSWLANLKNKAYIEIKFDNTPSG
                     QSTNALAPRPSPTSKP"
     gene            complement(6339..7214)
                     /locus_tag="VLD60_10075"
     CDS             complement(6339..7214)
                     /locus_tag="VLD60_10075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB45349.1"
                     /translation="MTRPPFDSTHLQVSRLFPLVIGAIGGTLLLLTSCTQPQSEERVV
                     AFVNGRSITQTEFDRKWGELPEATRARYEKEGGKQAFLKEVVDDELLMQEARKQGLDQ
                     TDSFRDRVRRYKEKLLKDELLKDRLKDTIDLTKEELDRYYVQHENELLTPLKVRVSQM
                     LLPNYPAAKDLETQINRGGDFAKFAQRYSIDTKTKAKGGDLGPYRKGLLVPEVDEAVH
                     ALKPGLVSGPIKTDAGYYLVMVTALEPEIIQADLARRERLRQELLYLKRKQHFDEVIA
                     DIKSKALIRISDGSR"
     gene            complement(7249..10710)
                     /gene="mfd"
                     /locus_tag="VLD60_10080"
     CDS             complement(7249..10710)
                     /gene="mfd"
                     /locus_tag="VLD60_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HSB45350.1"
                     /translation="MSNSSPGNPTTQWFSSLASSLAGEGGRPCLTGLHGSTAGFALTT
                     LLHPQSGKLGARSWLIVTKSDEAAERLYDDLQFFHGLIGLPASSLALFPRWETLPYES
                     TAPHVGLVARRMIALQQIRTLPSVRVITSIEALMQRLMPVETFTQTILHFKRGGAIER
                     DVLTGGLLRLGYRRVSVVEIPGEFSIRGGIVDIFSTAYTEPLRVEFIGESIDSLRLFD
                     PATQKSTAKMDRAVVLPAREYLRPEAAPDALATIPPDAEWRAPDLYPHMHSLLDYFTS
                     RPLLALNQPAALNRSCVELWDRIDDGYLRHSDQEAALPYPSPERLFLTWQDLMERTTG
                     WSQLALEPLAETDASWEPVLAFPAQVPASIGLGARGTPFSQTLSMLERLREACRVLLV
                     ARSHGQVDRLLALLREHDVPAIEWTTQSWTSSGQARVPFYVSHGDLSAGFLSSELHLA
                     ILTEEELFAKGARHKPQPKNKAATFLSSLEDLNVGDYVVHVQYGIAKYLGLKRLSVQD
                     FDSDYLILEFAGGDKLYVPLDRLNQVQRYSGAESHVPRLDRLGGTSWAKTTARVKKDI
                     EEMAQELIDLYANRELAKRHAYGFDSTLYHEFEAAFEYEETPDQLKAIEDIARDMESS
                     KPMDRLVCGDVGYGKTEVAMRAAFKAVEDNRQVAVLVPTTLLAHQHYDNFSERFAPFP
                     TKVALLSRFQSPKEAKAILKEVATGTVDVVIGTHRLLQKDVVFRNLGLVIIDEEQWFG
                     VKHKERLKELRTQVDVLTLTATPIPRTLQMAMASVRDLTIIDTPPAGRLSIRTQVVRS
                     SDKLIREAILRELSRGGQVYFVHNRVETMERTGAWIQQIAPEARIVMAHGQMDAKPLE
                     AVMLKFFHREADVLIASAIIQSGIDVPSANTIIVNRADTFGLAQLYQLRGRVGRSGDQ
                     AYAYFLVPDEGKLTDDAQKRLIAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHI
                     AAIGLDLYLQMVEQAVQRLKGQVVEEEPDPTLRLNVSAFIPEEYVADPHQRLSFYKRL
                     SSCTQVGDLALLHGELQDRYGLPPDAVERLFEVMQVRLQAKALRLSSIELKTQSIVIT
                     LDAKSPVAQTAIQCLMDRYRTRIRFLSPLSFELQMPHQDWPSIFPELTATLQSLSVCD
                     TNTTTKF"
     gene            complement(10895..11377)
                     /gene="rfaE2"
                     /locus_tag="VLD60_10085"
     CDS             complement(10895..11377)
                     /gene="rfaE2"
                     /locus_tag="VLD60_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HSB45351.1"
                     /translation="MPDKVMTSAQLAPVLAEARAQKRRIVFTNGCFDLMHIGHTRYLQ
                     AARQLGDLLVVGVNSDASVRSLDKKPDRPIVGEAQRAEVLAALGCVDYVIIFTEPDPL
                     KLISEIQPDVLVKGGDWPLDRIVGREVVERRGGIVKTIPLVPGMSTTSLIERIRSQSA
                     "
     gene            complement(11422..12579)
                     /locus_tag="VLD60_10090"
     CDS             complement(11422..12579)
                     /locus_tag="VLD60_10090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSB45352.1"
                     /translation="MRWARQILFGDVDAMFASAAVVKDPSLAGKLIAVGGPPPRGIIA
                     AASYAVRPFGIHSAMPTAKALQLCPNLILLPPDRPLYRHLHDHMRTVTNQLFPVTEWS
                     SIDEFYADTTDLQTRYPDPSALARLVKDSIFNATGLRCTVGVATGKTVAKIAADAYKP
                     DGLAIVEPDTEAAFLAGRPIRALPGIGPKTAARLEPLGLKTIGDLLDERWQPGLRHLF
                     GTRLHWIQELAQGHDHEPVVADRESKSASHETTFDQDTEDRSFLEQTLQIFLRSLARE
                     LRQEGLAAAACTVKLKDARFSITTRQRQFPQPLNYDPDMWPTIKQALHDLFKPGTKYR
                     LAGLALTSLTPAPPGLFDQKRAKAIEAMDAIISRHGPNVMGLGGVIRDDGD"
     gene            complement(12601..13239)
                     /locus_tag="VLD60_10095"
     CDS             complement(12601..13239)
                     /locus_tag="VLD60_10095"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HSB45353.1"
                     /translation="MARCPTHPLAAEQLTAIWSDLNKRHFGGVLPLIDIVWSRRLTSS
                     VGMFVSRAGPRSSWRDVVARRSPPREIRLSLPLLNGLLVRSPYGRNELVSTIAHEMIH
                     QWQYDILKRRPNHGPDFLRKMTEMNRDGALAITIYHSLQKEVLALARFAWRCRQCGRI
                     YRRQRRTIQPRRHQCGSCRGALQELGAVARSPVEPTAGPPPPLRQLQLSFTF"
     gene            complement(13248..14240)
                     /locus_tag="VLD60_10100"
     CDS             complement(13248..14240)
                     /locus_tag="VLD60_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45354.1"
                     /translation="MAKPPDRKRPPVTTRSLIPPINVTDLKTYPLRKRHSKVRVSDFA
                     RPWRRGGSFGQFYRTLPDILAVKTLRAVAKAIATAHCKGRPVIIGIGAHVIKVGLTPI
                     LVDLMQQGIVTAVAMNGAGIIHDFELALMGHTSEEVDAEIDAGRFGMAEETGRMLNEA
                     IVRGHRDRQGLGEAVGHYINRCKGQFPNRETSVLATGARLGIPVTVHVAVGTDIIHMH
                     PAADGAAIGATSLLDFRRLAAVVAGMEGGVYLNLGSAVILPEVFLKTVSLGRNLGHSL
                     SNITTVNMDFLSHYRPLTNVVRRPTQKGGKGYALIGHHEIMLPLLVAAVTEELG"
     gene            complement(14224..14802)
                     /locus_tag="VLD60_10105"
     CDS             complement(14224..14802)
                     /locus_tag="VLD60_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HSB45355.1"
                     /translation="MKDFVLNAFAESATVKQQFAREDVDRIVEVAKLIATAFREGHKV
                     LLFGNGGSATDAAHIAAEFVGRYKRERAPLPAIALATDIAAITCIANDYGYDELFARQ
                     VRAHGQKGDLAIAISTSGDSPNVLKGVEAAKACGLTTIAWTGGTGGKLAAMVDFPFVV
                     PSKVTARIQECHITLGHVLCELTEEQLLGKAS"
     gene            complement(14858..15796)
                     /locus_tag="VLD60_10110"
     CDS             complement(14858..15796)
                     /locus_tag="VLD60_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HSB45356.1"
                     /translation="MTGGLFVVLLLAVLVLIIIAKTAVVVPQQSAYVVERLGKYAATL
                     DAGFHILLPFIDRIRYKHSLKETAIDIPEQVCITRDNVQVSVDGILYLKVLNPQRASY
                     GISDYLFALIQLAQTTLRSEIGKIELDRTFEERTNINIQVVNELDKASDPWGVKVLRY
                     EIKNITPPKGVLDAMEKQMRAEREKRAVILTSEGERDAAINQAEGEKQQVIKASEAKK
                     QQQINEAEGAASAILAIAQATAEGLRKVAETIQVPGGQEAVQLRVAEQYITKFGELAK
                     VNNTLILPATVSDVGSMIALAMSAIRQTASSTPTNS"
     gene            complement(16087..16521)
                     /locus_tag="VLD60_10115"
     CDS             complement(16087..16521)
                     /locus_tag="VLD60_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSB45357.1"
                     /translation="MIWWYWMLVGLVLLGAEMITPGGFYILFFGLAALVVGGLAGLRL
                     VQTEWLQWLLFSGLAVISLLVFRGPLLAWIKAQEVETPAVDSMGGETAIPVEDLVPGG
                     IGKAELRGTIWTAHNAGPALLKKNQRCKVERVEGLTLWITAE"
     gene            16671..17927
                     /locus_tag="VLD60_10120"
     CDS             16671..17927
                     /locus_tag="VLD60_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HSB45358.1"
                     /translation="MAHDQPVAALVVAVTNDLSPAVYSINRLCPESLCFVLPESAKPL
                     VETEVQPKISQMPRRWDWIVLSDSDHFPSCYQSIARSLPGLLRTWGIEPGSLVLDLTG
                     ATPAMAGALALVTMRLTSRIVSIVASKEGREGDAVTIEGREGLWISTNPWDEAAEVMR
                     RDGCDLFNRGAYVAAGSIFRQIETRVSGGQKPLYRAFADVAEGYDFWERFHYRQAWEK
                     LKSSIKAMDMASLFGGPPGLKGAITSIKANAAFLEKLVLDPAEVKELLAYDLMAHAGR
                     RLRVTHDAEAAMQSLLRALEAFAQLQLFKQHKIKTWDAQPEQLPHAIQEICRTCYLED
                     IDGKYKLPLQAQFRVLAGLGDQMGQAFLREWPTMKPLLDAANHAVLGHGFEPVKAERV
                     QQLYDVVLKLTGVQDSALPKFPVLSL"
     gene            18013..18429
                     /locus_tag="VLD60_10125"
     CDS             18013..18429
                     /locus_tag="VLD60_10125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HSB45359.1"
                     /translation="MDIRIYYEDTDCGGVVYYANYLKYFERARTQYLEERGLSVAGLM
                     KEGRVFVVVHAEVDYLAPARYGETLQIQTVIAEMTPASITFSHIVCERESGRVVVEGS
                     ARLAVTDGNGKVKRLDKVTVSALQSSPTRGTERWIS"
     gene            19019..19249
                     /locus_tag="VLD60_10130"
     CDS             19019..19249
                     /locus_tag="VLD60_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45360.1"
                     /translation="MEMFGRVGLVEIPILIAPDQKVWMDKMIKEGKIAIPPGGTLEKG
                     SLYSMFIRMLLHNAMEEQKRQEALEADDDDDE"
BASE COUNT         3821 a         5448 c         5959 g         4112 t
ORIGIN      
        1 taagatcaga aatcggcagg acaaaccggt tgagacggtg gtggacttgc tgttcgaatc
       61 ggaagtcttg ctcgaggcgc aatcgttgga tcgagagcga gaagttcacc agcacatcgg
      121 cgatttcacg ctcttcatgg cgggcctctt tcccgaatac ctccgcaggc tgaagacggc
      181 gagcgtgatc taccataagg acttcctcgt ggattatgtt aaaaccggga agcggtccta
      241 tggtatcgtc gcgcagatgg ctgacggtcc ttctccggat gaaccgcctc tgttcaagaa
      301 actttccgac aacttcgaac tctgcgtcac cggcctgggc ttcgtgcgtt ccgatctgga
      361 tcgcatgaga gatccagctt tccgccaggc tcaggacctt ctcctcaatt gagccgttct
      421 cgccctctca ttctcgctca cggtggcgca ggccgccgtt cgatgaccaa gcgccaggca
      481 tcctgcctcg ctgaggctct cacgaccggc tatgctgtgc ttcaacgggg tggttccgcg
      541 gtcgaggccg tggaatgcac gatcggattg ctcgaggcct ccggattctt caacgcgggg
      601 cgaggatcga accggcagct ggacggggtg cagcgaatgg atgcctcaat tatggaaggg
      661 gctcgtctga aggccggcgc ggtggcgtcg attgaaggca ttgtccatcc gattactgcc
      721 gccagactcg tcatggaaga aacagatcat gtcttgttgg tcggacgacc agccggcaga
      781 ttggcacagc atttcaagct cgagcggcat cactcaggtc atgcgccgcg ccagtgtgcc
      841 tatgacaaga ttttcgacag gcagaaacat tcaagagcag acaagcacgg cactgtcggc
      901 gccgtagcct tggacgagtc gggaacagtt gcggccggcg cgtcaaccgg agggatccag
      961 tcgatgttgc ctggccgtgt cggcgatacg ccgctgatcg gatgcggcgt ctatgcagac
     1021 aatcgttccg gcgccgtgtc catgaccggt cttggagagg gtatcatccg cttggcgatc
     1081 gcgaagacga tctgcgaacg gctggctgcg gggaagagcc cgatcatggc ggccaggcag
     1141 gtgttgcgtc aactggtctc gcgaatcaat ggtgcggcgg gtacactggt agtatcaccg
     1201 gacggacgct tcgccattca ccacgtcact ccgcacatgg cggccggctg gtggaagggg
     1261 tctggacggc ctatggtcaa ggggcggttc gcatgagggg ggcaatcttt cacctagcct
     1321 tcccgacgca cgatctggtg gcagccaagc aattctatgt tgacggactc ggctgtacgc
     1381 tggggcgtga atcgagtcgg gccgtgacat tcgggttggc cggtcatcag cttgtcgcgc
     1441 atctggtcga cgaatctcct cccgtgcaac aggggattta tccgcgtcat ttcgggctgg
     1501 tgtttctctc gaaagtcccg tggcaggaat tggttgagag ggcgaaggca aacagactcc
     1561 agttctatca acagccgcgg gtgaggttcc ctgggacgcg tctcgagcac cacacgttct
     1621 tcctgcagga tccctcccag aatctcctcg aattcaaaca ttacacgcac gaatcggcga
     1681 tcttcggcga gcatgacagc cgtatggtcg gcgatacctc ggatcctgat tgacggtcaa
     1741 tactcggcgc ggatcccgcc catgacgagg agcgggctgc cgagtgtctt gactggaccg
     1801 gtctgcgtgg cgatgtagtg ccggtcggtg aggttttcac cgttgagcac cagccggagg
     1861 cctttgcgaa gctggtgctg gagcgaggcg tcgagcacga cgaaatcggc gaccggctgg
     1921 gtattgaagt cgtcggtgaa ttggcgcgag agatagcggg ccatgatcgt gacttccgcc
     1981 cagtcggccc ggccacccgt catgccgacg ctcagctggt tccgtgagac gttcgggatg
     2041 cgcttccctt cccggtcctg cgccccggga aaactcgtga tggtcgaatc gacgtaggca
     2101 tagccgaaag tgagcgagag gagcgggctg acgcgatatg agagatccaa tgccccaccg
     2161 gtggagagag ccttcccgac attctgcctt tgcgcagcca atggtccttg cgagacgaag
     2221 agaatctgat ccttcacctc gtcacgatgg accgagaggc gccagcgaag ggctcggtca
     2281 tcaaatagtt cgccttccag tttgacctcc cctcctgtca gtcgctccgg gcgaagctga
     2341 tcgttcggga gaaaactaaa gccggcgaat gcaaaacctc gataaagttc gttgatcgtc
     2401 ggggcccgaa acccttgata gaccgaggca cctgcccgaa cgcggtcggt caggacatag
     2461 ttcaccgaaa gtttcggatt gacgacgctc accgtgttgt cttcaaatgc tgtggtagtt
     2521 gtcgtagagg caccgcgggc attgaagttt ttccaccaat cggcccggac gctcgggatc
     2581 acggtcagcc gccccgttgc cggcaggatc cattctccaa acaggcccca tccgatctgc
     2641 tgtcctctgg ccaatgtctg gccggtgggt ccactcgagg aaaagagatc gtcggtggtc
     2701 tgcgcaatga ttgttcgagc atccgtgccc aacaccagac gatccggtcc ccacaaactg
     2761 ccggtccatt gtagtgaccc gccgaagtcg ttgctgggaa tagtctgaat cacgttttga
     2821 aactccgcga ggcgcagaag cggggcaggc gtaatctggg acgtcacgtt tcgaaaggtc
     2881 tgccattggc caaagagttt gctttcccac aggccggcct gaccctggtc gctccggatc
     2941 ccaagcgcca cattaccgat ggtcctcgat gccagattga ggttcgttcc gaaattgcga
     3001 tcttcacgaa agaggcttcc cgataagtcg atcgtcgtgg ctcccgaggg cgtccaggcc
     3061 accctgccgt tgaacagttg atgcctcgaa tcgatcgttc ggtccaccgg tcctcgttgg
     3121 taggccggga caggaatgaa gccgttcgtg tggaaccacc gatagcccag cgagaaccgg
     3181 aaccggtctg ttccataccg cgcactcaag gcttgttgat aggtatccag tgtaccggcc
     3241 tgactgtcca gcttgaatcc catcccttcg ggcggggttt tcgtgatgac gtggacgacg
     3301 ccccccatgg cccatgtgcc gtagagattc gaggttccgc cgggaacgat ctccacgcga
     3361 tcgatatcgt tcgggatcag tccccaattg atccatcctc cgaacccgtc gtttagcgga
     3421 atgccatcga gtaacaccaa ggtccgacga actcccaggc cgttcaaggt tatagactga
     3481 gccgtgggat ggttgtagcg gctgctgagt gtggccggct ggacgccggg aaccgatctg
     3541 agcagatcat ccagctgatg accgccggca tagggcgacc gctcgatctg ttctcgcgtg
     3601 agcacggtaa ccgctgaagc gctttggctg accggttcca ccgtcctcgt cgcggtaatg
     3661 accacctcgt ctgtcgtcaa gatcgaaggt tcttgggagg actcttcggc ggccgcggga
     3721 ctgaagatca tgcacgtcgc tgcaagggag cagaagcgaa tgatggaacg aaccctgacg
     3781 ctgaatcctc cgtctctaat cggggcggat gtcatgacgc cccagctggt tcattcgacg
     3841 gaggatgcga tcgtagcggc gggtcaacgc tgcggttttg tgtaatgggt catatcgccg
     3901 cggagcaggc aggatggctg ccagccaggc tgcttcgtcc gtcgtcaact cacgtgcagg
     3961 tttcttaaag tggtggcgcg ccgccgcctc agctccatat acgccgtttc cccattcggc
     4021 gacattgaga tagagttcga ggattcgatc tttcgtcaag tgatgctcca ttgaacgcgt
     4081 gatcagagcc tcgcgggctt tccggaagat cgatcgttcg gcggagagat agagattctt
     4141 ggccaactgc tgggtgatcg tgctgccgcc gcgcttcagt tcgcctttct caagatcgta
     4201 gagggcagca tctttcatgc cctcccaatc gaacccctca tgcgtgaaga acgaggcgtc
     4261 ttccgccgca acgacggcgc ggcggaggtc gggggctatc cgcgagagag gcacccagat
     4321 ccagtggcgt ccgatggact ggcctttgat tcgagcctgg tactcacgtg cctcgatgag
     4381 agcggtcatc tcagggttga ccttttgcag cgccgggatg tccggcatcg tgatcagcca
     4441 gataaacagc aaggctgcga tcggaagcgc gatgcaaagg aacagaatga gggtcaagcg
     4501 tcggcgttgt ttggttgact tccgcattgt gggctgcgta tgagaaggga cgaaacgaga
     4561 gactcggctc cgaagggttt cacgaccgtg cggcccttct cgccgtctcc attcccagtt
     4621 tttttgcctt ggagttcccg tgaaaattct gaccgcagag tttatcagaa gttgcgtcgg
     4681 cccagaacaa tttccgagag gaacggtgcc agaaattgcc ttcgtcggcc gctccaacgt
     4741 cggcaagtcc tcgctcatca attccttgct gcaacggaaa ggactggcca aggtcagccg
     4801 cacgccggga aaaaccaggg cggtcaatct cttcaatgtc gcgaccgatg accccggtct
     4861 ctcgcgtttc gtgctcgcgg accttccggg ctacggctat gcaaaagtct cgaagacgga
     4921 acgggctcaa tgggccccat tgattgagca gtatttgagc caacgcgagc agatccgggc
     4981 gatcgtgatg ttagtcgaaa gtcgcgtgct cggcgctcat gacggggaaa caatcgcctg
     5041 gctgcgatcg atcggtctgg aaccgctggt cgtggctacg aaagtcgaca agttaaaacc
     5101 cagtgaacgc gtctcggccc tccgtcgact tcaggaagga ctgggggtga ccgacagtcg
     5161 cacattcatg gtttactcgt cggtgacggg cgaggggcga gagcgtttgt ggactgccct
     5221 gaaggagtgt tgtcgaactt gatctcgatg taggctttgt tcttgagatt cgccagccag
     5281 gactgaaaca tatcctcgct cttctgctgg aagacgagca cttggatctc ctgcttcact
     5341 tcttcatagg gcctgaatac cttcggcttc ttatcatcga cacgaacgat gtgcaggcct
     5401 tcggggcttt gaaggggctc cgagatgcca ccgggcacaa gactcgcgac gccccgctcg
     5461 atggcgggca ataactctcc ctgccgtacg aggccgagtt tccctccgcg tgacgcattg
     5521 gggccgtcgg agtaacgcag cgccagatcc tcgaaggact ctccctgctt gagcagggcc
     5581 atgacctctc gggccttgtc tttggcgtcg gccagatcgt caggcgagcg gggtttgagg
     5641 aatatctggc tgagcgtata ttcttcaggg agcgcaaacc ggtcgcggtg ctcctggtag
     5701 taccgcctca tctccgggtc tgccaccatc acgccgcttc gcacttcccg atcgacgacc
     5761 ttgaggagaa tcagttgttc gcggacgctt ttgatgcctg ccggatcttt gtcgtcaagt
     5821 ttctctcctt gctgattcaa ttgcttgatg gcctgcttca cctcctggtc gctgacatcg
     5881 atgccacgga ctttggcttc ctgaatctgc agtcggcgct caatcatcgt cgtcagggcc
     5941 atcgattccg ccgccttcag cttgcgcaat aattcctcgc cgcgatactg cttttcgatg
     6001 cgctcctgct cggggctcag ttcccgtttc atttccgacc acatgatcag atcggagttg
     6061 acgatcgcca cgatgcgatc ttcgatcttc gccgctcgaa gatctggcac tccggcgaac
     6121 agggtcaagg cgagtgtcgc agtcgcgagc gaggcaaacg caatcgatgt gattcgagag
     6181 cgggtcatgg ctttgcggag ttcggtatgg atcggcatgg ctgtatgtgc cggaggaatc
     6241 tgtccatcgc ggatgaattg tacaggattc tctgcgtgat gaggaaggga aagaccgcgg
     6301 tgctgtcggc tcgcgtgctc gcagagccct cggttcgtct agcgagaccc gtcgctgatt
     6361 cgaatgagcg ccttggattt aatgtccgcg atgacctcgt cgaagtgctg cttgcgcttg
     6421 agatagagca gttcctgtcg caagcgctcc ctgcgcgcga ggtctgcctg gatgatttcg
     6481 ggctcgaggg ccgtgaccat gacgagatag tagccggcgt ccgtcttgat gggtccgcta
     6541 accaacccgg gtttgagcgc gtggaccgct tcgtcgactt caggaacgag caagcccttg
     6601 cgatacgggc ccaggtcgcc tccttttgcc ttggttttgg tatcgatcga atagcgctgg
     6661 gcaaactttg cgaagtctcc tcctcgattg atctgggtct ctagatcttt cgcggcaggg
     6721 tagttcggga gcaacatctg cgagacgcga actttcaaag gggttaagag ttcgttctcg
     6781 tgctgcacgt aatagcggtc cagttcttcc ttcgtgagat cgatcgtatc tttcagcctg
     6841 tccttcagga gctcatcttt caggagcttc tccttgtagc gccggactcg atcgcggaac
     6901 gagtcggtct ggtcgaggcc ttgcttcctg gcttcctgca tcagcagctc atcatcgacc
     6961 acttctttca aaaatgcctg tttcccgcct tctttctcat aacgggctct ggttgcttcg
     7021 ggaagctcgc cccatttccg gtcgaattcc gtttgggtga tagaccgtcc gttcacgaag
     7081 gccacgacac gttcctcgct ctgaggctgg gtgcagctgg tgagcaacag cagcgtgcct
     7141 ccgatcgccc cgatgacgag aggaaagagc cgggagacct gaagatgagt ggaatcgaac
     7201 ggtggtcgtg tcatgcgcgt gtgcaacctt gtcggggttg aaggacggtt aaaacttcgt
     7261 tgttgtgttg gtatcacaga cactgaggct ttgcaaggtt gcggtgagtt ccggaaagat
     7321 ggaaggccag tcctgatgcg gcatttggag ttcgaaggac aagggagaaa gaaaccggat
     7381 tcgggtccta tatcgatcca tgaggcattg aatcgcggtc tgggcgaccg gactcttggc
     7441 gtccagcgtg atgacgattg attgcgtctt gagctcgatc gacgagagcc gcagtgcctt
     7501 agcctgcaat cgcacctgca tgacttcgaa taatcgttcg acagcgtcgg gcggcaaccc
     7561 gtagcgatcc tggagctcac cgtgaagtag ggcgagatcg cccacttggg tgcaggagga
     7621 gagccgttta taaaatgaca ggcgctgatg gggatcggcc acatattctt ctggaatgaa
     7681 tgccgacaca ttcaggcgca aggtaggatc gggctcttct tccacgacct ggcctttgag
     7741 ccgctgaacg gcctgttcga ccatctgcaa gtagaggtcg agaccgatgg cggcaatgtg
     7801 tccggactgt tgcttgccca gcaagttgcc cgcgccccgg atttcgagat ccgcggcggc
     7861 gatgcggaaa cctgatccca gctccgtaaa ttgctggatg gcgattaggc gtttctgcgc
     7921 gtcgtccgtc aacttgcctt catccggaac gaggaaatag gcataggcct ggtctccgct
     7981 tcggccgaca cgcccgcgca gttgatagag ctgtgccagg ccgaacgtgt cggctcgatt
     8041 cacgatgatc gtgttggcac tcggcacgtc gatgccggat tgaatgatgg cggaggcaat
     8101 caggacgtcc gcctcacgat gaaagaattt cagcatcacg gcttccagcg gcttcgcatc
     8161 catttgaccg tgggccatga cgatgcgtgc ctccggggcg atttgttgta tccaggcgcc
     8221 cgtgcgctcc atcgtttcga cacgattgtg gacgaaatag acctgcccgc ctcgactcag
     8281 ttcgcgcagg atcgcttcgc ggatcaattt gtcgctggac cggaccacct gtgtgcggat
     8341 cgagagccgg ccggccggcg gcgtgtcgat gatcgtaaga tcgcggacgc tggccatcgc
     8401 catttgtaac gtccggggaa tcggcgtcgc cgtgagagtc aacacatcga cttgtgtgcg
     8461 caattccttg agtcgctcct tgtgtttcac tccgaaccac tgttcctcgt cgatgatcac
     8521 gaggccgagg ttgcgaaaca ccacgtcttt ctgcaacagc cgatgtgtcc cgatgacgac
     8581 atcgaccgtg ccggtcgcca cctctttcaa gatggctttg gcctctttcg gtgactgaaa
     8641 gcgggagagc agggcgacct ttgtgggaaa gggcgcgaag cgctccgaga agttgtcgta
     8701 atgttgatgg gccagcagcg tcgtcggtac cagcacggca acctggcgat tgtcctcgac
     8761 ggccttgaag gcggcgcgca tggccacttc tgtttttcca tagccgacat ccccgcagac
     8821 caggcgatcc atcggcttac tggattccat gtcgcgcgcg atgtcttcga tggctttgag
     8881 ctgatccggc gtttcctcat actcgaaggc cgcttcgaac tcgtggtaga gcgtgctatc
     8941 gaatccgtac gcgtgccgtt tggcgagctc gcgattggca taaaggtcga tcagctcctg
     9001 cgccatttct tcgatgtctt tctttacgcg ggcggtcgtc ttggcccaac tggtaccacc
     9061 caatcgatca agccgcggga catgcgactc ggcgccgcta tagcgttgca cctgattgag
     9121 gcgatccagc ggcacataga gcttgtcccc gccggcgaat tccagaatca ggtaatcgct
     9181 gtcgaagtcc tggaccgaca gacgtttgag ccccaggtac ttggcgatgc catattgcac
     9241 atgaacgacg tagtcgccca cgttcagatc ttcgagcgag gagaggaagg tggcggcctt
     9301 gtttttcggt tgaggcttgt gacgggcgcc ttttgcaaag agctcttctt ccgtcaggat
     9361 cgccagatgc agctcggacg agagaaagcc ggcggagagg tcaccgtgcg agacgtaaaa
     9421 tgggacgcgt gcctgcccgg acgaggtcca gctctgcgtt gtccactcga tggccggcac
     9481 gtcatgttcg cgcaggagcg ccaagaggcg atcaacctgg ccgtgactcc tcgcgaccag
     9541 gaggacgcgg caggcttcac ggaggcgttc aagcatcgac agcgtctggc tgaacggcgt
     9601 gccccgcgcg ccgagtccga tgctggccgg aacctgtgcc ggaaaggcca acaccggttc
     9661 ccacgaggcg tcggtttcgg ccagcggctc aagggccagt tgggaccagc cggttgtgcg
     9721 ctccatcaaa tcctgccacg tcaaaaacaa tcgttccggg gaggggtagg ggagcgccgc
     9781 ctcttgatcg ctatggcgca agtagccgtc atcgattcga tcccacaatt cgacgcacga
     9841 gcggttgagg gcagccggtt gattcaacgc gagcaaaggc cggctggtga agtaatccag
     9901 cagactgtgc atgtgcggat aaaggtccgg cgcgcgccat tcggcatcgg gagggatcgt
     9961 tgcgagcgca tccggagcgg cctccggccg aagatactcc cgtgctggaa ggacgacggc
    10021 gcgatccatc ttcgccgtgg atttctgtgt ggcgggatcg aagaggcgga gcgagtcgat
    10081 gctctctccg atgaattcca cacgcaacgg ttccgtatag gccgtcgaga aaatgtcgac
    10141 gatgccgcct cgaatgctga actcaccggg aatctccacg accgaaaccc gacggtaccc
    10201 caatcgcaaa agcccaccgg tcaagacgtc gcgctcgatt gcgccgccgc gcttgaaatg
    10261 caggatggtc tgcgtgaacg tctcgacagg catgagtcgc tgcatcaacg cttcgatcga
    10321 ggtgatgacc cggaccgacg gaagggttcg aatctgctgc agggcgatca tccgccgggc
    10381 cacaagaccg acatgcgggg cggtcgattc gtagggcaga gtttcccatc gcgggaatag
    10441 tgcgagcgag ctggcgggaa gaccgatcag accgtgaaag aattgcagat cgtcgtagag
    10501 tcgttcggcg gcttcatccg acttggtgac gatcagccac gatcgggcgc cgagcttacc
    10561 cgattggggg tgcaacaatg tggtcagcgc gaacccggcg gttgaaccgt gcaagccggt
    10621 caggcagggt cgtccccctt caccggccag tgaggaggcg agtgacgaaa accactgggt
    10681 cgttgggttg ccgggcgagc tgttcgacac tgcttagccc gcactattca tgacggttca
    10741 tcgcgaaatc gccaagaccc gtcgtgaaac gggcgcttgg agccgtgagg gagggagaca
    10801 tactcgtgta gtacgttgac cgactgagcg gcgagactgc ctgttgcagc agcgtatggg
    10861 tgattgcagc aaaagtgctc atgaataatg cgggctaggc ggattgtgag cgaatacgtt
    10921 cgatgagtga cgttgtggac atgccgggaa ccaacggaat cgtcttgacg attccacccc
    10981 ggcgttcgac gacctcacgg ccgacaatgc gatccagcgg ccaatccccc cctttcacca
    11041 gcacgtccgg ttggatctcg ctgattaatt tcagagggtc cggctccgta aagatgatca
    11101 cgtaatcgac gcagcctaat gccgcgagca cttcggctct ttgggcctct cccacaatcg
    11161 gccggtccgg ctttttgtcg aggcttctga cggaggcatc gctgttgacc cccacgacga
    11221 ggagatcgcc aagttgtcga gccgcctgca ggtagcgcgt atgcccaata tgcatcaggt
    11281 cgaagcatcc gttcgtgaag acgatccgcc ttttctgggc acgcgcttct gcgagaaccg
    11341 gagcaagctg cgcgctggtc atcaccttgt cgggcatggt ttcatcatac cggctgcgcg
    11401 acgagataga aagagtggag gttagtctcc atcgtcgcgg atcaccccgc ccagccccat
    11461 tacgttcggt ccatggcgcg agatgatcgc atccatcgcc tcgatggctt ttgctctttt
    11521 ttgatcgaag agaccaggag gagccggcgt caatgatgtg agggcgaggc cggcaaggcg
    11581 gtacttcgtt ccgggtttga acaagtcatg cagtgcctgc ttgatggtcg gccacatgtc
    11641 agggtcgtag ttaagtggct ggggaaactg ccgctgcctc gtggtaatgg aaaagcgggc
    11701 gtctttgagt ttgacggtgc atgcggcggc ggccaggcct tcctgtcgca gttcgcgcgc
    11761 caaagagcgc agaaatatct gtagcgtctg ctcgagaaac gaacggtcct ctgtgtcctg
    11821 atcaaaggtc gtctcatggc tggcgctttt tgattcacgg tcggccacga ccggttcgtg
    11881 gtcgtggcct tgagccaatt cttggatcca atggaggcgg gtcccaaaga gatgccgaag
    11941 ccccggttgc catcgttcat ccaaaagatc gccgatggtc ttgagcccga gcggttccaa
    12001 gcgtgcggcg gttttcggac cgatgccggg gagggctcgg atcggccggc cggccaggaa
    12061 cgcggcttcg gtgtccggct caacgatcgc gaggccatcc ggcttatacg catcggccgc
    12121 aatcttcgcc acggtctttc cggtcgcgac gccgactgtg cagcgcagtc ccgtggcgtt
    12181 aaagatggaa tctttcacca atcgagccag tgctgaagga tcgggatagc gggtctgaag
    12241 gtccgtcgtg tccgcgtaga attcgtcgat gctactccac tcggtaacgg ggaagagctg
    12301 attcgtcacg gtccgcatgt gatcgtggag gtggcgatac aacgggcggt caggcggaag
    12361 caggatgagg ttcggacaga gctgcagtgc cttcgccgtg ggcatggccg aatgaatacc
    12421 gaatgggcgg accgcgtagc tggccgcagc gataatgcca cgcggcggag ggccgccgac
    12481 cgcgatcagt tttccggcaa gactcgggtc tttcactacg gctgcggagg cgaacatcgc
    12541 gtccacatct ccgaagagga tttggcgggc ccagcgcatg attggctcca gagaaggagc
    12601 ctagaacgta aaggagagct gaagttgacg caaggggggt ggtgggccgg ccgttggctc
    12661 gacggggctt cgggcaacag cacccaattc ctgcaaggcg ccacggcaac tcccgcattg
    12721 gtggcgacgt ggttggatcg tccggcgttg gcgtcgatag atgcggccgc attgccggca
    12781 gcgccaggcg aatcgcgcga gagccaatac ttccttctgg agtgaatggt agatggtgat
    12841 ggccaaagct ccgtcgcgat tcatctccgt catcttccgg agaaaatctg gcccatgatt
    12901 tgggcgtcgc ttgaggatgt cgtactgcca ctgatgaatc atttcatggg cgatggtgct
    12961 gaccagctcg ttccggccgt agggggatcg gaccaacagt ccgttgagca ggggaaggga
    13021 caggcggatt tctcgcggtg gagaccgtct cgcgaccaca tcgcgccagg aggacctggg
    13081 tcccgcgcga ctcacgaaca ttccgaccga tgaagtgagc cgccggctcc agacgatatc
    13141 gatcaaggga agcacgccgc cgaagtggcg cttgttcaag tcggaccata tggcggtgag
    13201 ttgttctgct gctaggggat gagtggggca gcgggccata gcgggcctta tcccaattct
    13261 tcagtaacgg ctgccaccag tagcggcagc atgatttcgt ggtgaccgat cagcgcgtaa
    13321 cccttcccgc ccttttgagt cggccggcgg accacattcg tgagcggtcg gtagtgagac
    13381 aagaaatcca tgttcacggt cgtgatgttg gacaggctat ggccgaggtt tcgacccagt
    13441 gagacagttt tgagaaacac ttctggcaga ataaccgcgg agccgaggtt gaggtagaca
    13501 ccgccttcca tgccggcgac gaccgccgcg aggcggcgga agtccaggag cgaggtggcg
    13561 ccgatggccg cgccatccgc ggccggatgc atatggatga tgtcggtgcc gactgcgaca
    13621 tgcaccgtga cgggaatgcc gagccgggct cccgttgcga ggacgctggt ttctcggttg
    13681 gggaactgtc ccttgcacct attaatgtag tggccgactg cttcaccgag tccctgccga
    13741 tcccgatgtc cccggacaat cgcctcgttc aacattcggc ccgtctcctc cgccatgccg
    13801 aaccgcccgg cgtcgatttc ggcatctact tcttccgacg tgtgacccat cagggccagt
    13861 tcaaaatcgt gaatgatgcc cgcgccgttc atcgcgacgg cggtgacaat cccctgctgc
    13921 atcaaatcca cgagaatggg ggtcaggccg actttgatga catgagcgcc aatgccgatg
    13981 atcacgggcc ggcctttgca atgggccgtg gcaatggctt ttgcaacggc gcgaagcgtc
    14041 ttgacggcca gaatgtccgg caaggtgcga tagaactggc cgaagctgcc tccgcggcgc
    14101 cagggcctgg caaaatcgga gacacgcact ttgctatggc gtttcctgag cggataggtc
    14161 ttgagatccg tcacgttgat cggcgggatc agactgcggg tcgtgacggg gggacgtttc
    14221 cggtcaggag gctttgccaa ggagctgttc ctcagtcagt tcacacaaga cgtggcctaa
    14281 tgtgatgtgg cattcctgga tgcgcgcagt cactttggaa ggaacgacaa agggaaaatc
    14341 caccatcgcg gcgagcttac caccggtccc gccggtccac gcgatagtgg ttaagccgca
    14401 ggccttcgcc gcttctaccc ccttgagcac gtttggcgaa tccccgctcg tgctgatcgc
    14461 aatggccaga tcgccttttt gtccgtgggc gcggacctgc cgggcaaaga gctcgtcgta
    14521 gccgtagtcg ttcgcgatgc acgtgatggc ggcaatgtcg gtggcgaggg cgatggccgg
    14581 aagcggggcg cgctcacgtt tgtaccggcc cacgaattcg gcggcgatgt gggccgcgtc
    14641 ggtggcgctg cctccatttc cgaagagcaa cactttgtgg ccttcgcgga aggcggttgc
    14701 gatgagtttt gcgacttcta cgatccggtc gacgtcttca cgagcgaact gctgcttgac
    14761 cgttgcgctc tcggcaaaag cattgaggac gaaatctttc atcgggttca attctaggtg
    14821 aggggtctca tcctgtcaaa ccaggctatt tcaatggtca cgagtttgtc ggggttgagg
    14881 aagccgtctg tcgtatggca ctcatcgcca acgcgatcat tgagccgacg tcggagacgg
    14941 tggctggcag gataagcgtg ttgtttactt tggccagctc gccgaatttg gtgatgtact
    15001 gttcggcgac ccgcagctga accgcttcct ggcctccagg aacctgaatc gtctcggcga
    15061 ccttgcgcag tccttctgcc gtggcctgag caatggcgag aatggccgaa gcggctccct
    15121 cggcctcgtt gatctgctgt tgcttcttgg cttccgaggc cttgatgacc tgttgctttt
    15181 ccccctccgc ctgattgatt gcagcgtcgc gctcgccttc tgacgtcaag atcaccgcgc
    15241 gcttttctcg ctccgcccgc atctgctttt ccatcgcatc cagcacgccc ttcggcgggg
    15301 tgatgttctt gatctcgtag cgtaagacct tgacgcccca tggatccgag gccttgtcga
    15361 gttcgttcac gacctggata ttgatattgg tccgttcctc gaacgtgcgg tcgagttcga
    15421 tttttccaat ttcgctccgg agcgtcgttt gcgcaagctg gatcagggcg aaaagatagt
    15481 cactgatgcc ataggacgcc cgttgagggt ttaagacctt gaggtacaag atgccgtcca
    15541 ccgatacctg cacgttatcg cgcgtgatac agacctgctc cggaatatcg atggcggttt
    15601 ccttgagcga gtgtttgtag cgaatgcggt cgatgaaggg gagaagaata tgaaagcctg
    15661 catccagggt ggcggcatac ttgccgagcc gctcgaccac ataggcgctc tgctgcggca
    15721 cgaccaccgc ggtcttggcg ataatgatca agaccagcac cgccaacaac aacaccacga
    15781 agagtccgcc ggtcatcgtt acctcctcca cagtcgtttg ctttctagca ggatgctgaa
    15841 aaagtcaccc gtctcacccg cccagccccg gcgcgccaat acgcaccttg accctaccgt
    15901 cgttctcgca tcgttcagac cctcaacgta cccagagggt acgcctcggc ccttcactcg
    15961 ctgcggcctc gctggacggc cattttgagc atcctgcgga gtagtatcct gttatccctg
    16021 aggacggacc actgaacgtc tggaccgcca ctgtagtttt tccgcggtct tccggccact
    16081 tctcgctcat tcggccgtga tccataacgt gagcccttcg actcgctcga ccttgcatcg
    16141 ctggttcttt ttcaagagag caggcccggc attatgggcg gtccatatgg tgccgcgcag
    16201 ttctgccttg ccgatgccgc ctgggaccag atcctcgacc gggatcgcgg tttcgcctcc
    16261 cattgaatcc acggccggcg tctcgacctc ctgcgccttg atccaggcca gcagcggtcc
    16321 acggaagacg agtagcgaga tgactgcaag cccggaaaat aacagccact gaagccattc
    16381 cgtctggacg agtctcaagc cggccagccc gcccacaacc agtgccgcaa gaccgaagaa
    16441 gagaatataa aagcctccgg gcgtgatcat ctcggcaccg aggagcacga gaccgaccag
    16501 catccaatac caccagatca tgggaaaacc tcgtcgagcc ttgcgtaacg taacggattt
    16561 gtcgtgatga cgacgaaagt ctaagcgcct ttccagaggt cgtcaagcag aaggacgctg
    16621 cgggattgtg aattgatcac ctggagaggc gatgttatga tccgcgcgcg atggcccacg
    16681 accaaccagt tgccgcgctc gtcgtcgcgg tgaccaacga tctttctcca gcggtctatt
    16741 ccattaatcg actgtgccct gaatccctgt gctttgtgct gcccgaatct gccaagccgc
    16801 ttgtcgaaac cgaagtccaa cccaagatca gccagatgcc ccgccggtgg gattggatcg
    16861 tgctttcaga ttcagaccac ttcccgtctt gttatcaatc aattgcacga tcgcttcccg
    16921 gcctgctgag aacgtggggc atcgaaccgg gatcgctagt cctggatctg accggagcca
    16981 cccccgccat ggccggagca ctcgcgctcg tcaccatgcg cttgacgtct cgaatcgtgt
    17041 cgatcgtggc gtcgaaggag gggcgggaag gcgatgcggt gacaattgaa ggtcgtgagg
    17101 ggctgtggat ctccaccaat ccctgggatg aagcggccga ggtcatgcga cgagacgggt
    17161 gtgatctgtt caatcgaggc gcctatgtgg ctgccggatc catcttccgg cagattgaaa
    17221 cgcgcgtaag cggcggacag aagccgctgt accgagcttt tgccgatgtg gcagagggct
    17281 atgatttctg ggagcggttc cactatcggc aggcctggga gaaattgaaa agctctataa
    17341 aggcgatgga catggcctcg ctgttcggcg gacctccggg gctcaaggga gccattactt
    17401 cgatcaaagc gaacgcggcg ttcttggaga agctggtgct tgatccggca gaggtcaagg
    17461 agctccttgc ctacgatctt atggcccatg ccggtcggcg gcttcgagtc acgcacgatg
    17521 ccgaagcggc gatgcagtcg cttttgcggg ctctcgaagc ctttgcgcag ctgcagctct
    17581 tcaagcagca caagatcaag acttgggatg cgcagccgga gcaattgccg catgcaattc
    17641 aagagatctg ccggacctgc tatctcgaag acatcgacgg taagtacaaa ctgccgttac
    17701 aggcgcagtt tcgcgtgctc gccggattgg gcgaccagat gggccaggcg ttcctccggg
    17761 aatggccgac catgaagccg ttgctcgatg ccgcgaatca tgccgtcctc ggccacgggt
    17821 ttgaaccggt gaaagccgaa cgcgttcagc agttgtacga tgtggtgctg aaactcacag
    17881 gagtacagga cagcgcgctg ccgaagtttc cggtgctgag cctgtagcgc gacgtgtgag
    17941 attggcgcga aaagcgagac tagcatcgtc cgtcaagtcg cgctcgtccc gcctgcctcg
    18001 cgagtagaga gcatggacat tcgcatttat tatgaagaca ccgattgcgg cggcgtagtt
    18061 tactacgcca attacctcaa gtatttcgag agggccagga cacagtacct cgaggagcga
    18121 gggctctcag tggccggact catgaaggaa ggaagagtgt tcgtcgtcgt gcatgccgaa
    18181 gtggactatc tcgctcctgc acgctacggc gagacgctac agattcagac ggtcatcgcc
    18241 gagatgactc cggcatcgat caccttctcc catatcgtgt gtgaacgcga gagcgggcgt
    18301 gtggtcgttg aagggtccgc gaggcttgcg gtgacggatg gaaacggaaa ggtgaaacgc
    18361 ctcgacaaag ttaccgtctc cgcactacag tcgagtccga cgagagggac agagagatgg
    18421 ataagttagg aacctggctg gcgatcacgg gcgttgcgat cggatttatc gtgctgggct
    18481 ggctgctgtt ttcggagggt ccgaaggaca agaacaagaa acagtaagct cctcccttct
    18541 ttcgtgcggc attattcctc tcatggtcgt tcatcctgct caagcttgcg gtgaggcaac
    18601 tggctcctga gactccgctg tgatgacctc gaaagcccgt ttgctttctt gtggggatgg
    18661 agatcctgct cccggtaacg gcgatggggc ttgagatcgc tgccggcttg cgcggttggt
    18721 tgccagcaaa gctcacagga ggaaggagag ccctcggcat cgctgttagg cgatagcggg
    18781 gttgtgggca acgatgacgg atgggcggtc gattgctgcg cctgcgcacg aagcggcagc
    18841 agcaggcaga acgccgccaa tgaaagggcg aagcagggaa ccccggtaca aagaaaacgg
    18901 tgtgccatgg cagctggata cgcagggttc gtgccatagg cgttcacctc cgagttgaca
    18961 accgagaaaa ccgcatgctg gaatgaccgc ggagtcgaaa tctacggagg ttctgaccat
    19021 ggaaatgttt ggacgggtcg gcttagtcga gatcccgatt ctgatcgcgc cagatcagaa
    19081 ggtatggatg gacaagatga tcaaagaagg gaagatcgcg attccgccag gggggacgct
    19141 cgaaaagggc tcactctatt cgatgtttat ccgcatgttg ctccacaacg cgatggagga
    19201 acaaaagagg caggaagcgc tcgaggcgga tgacgacgac gatgaataaa gcaggatgag
    19261 gagtgggctc tccactggcc ctgcggggct cgataactgt atcgaaagcg attcgaagga
    19321 tatctccctc gatacagccg
//
LOCUS       DATIFR010000176        15021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2030837, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000176 DATIFR010000000
VERSION     DATIFR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15021
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030837"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VLD60_10135"
     CDS             complement(<1..1091)
                     /locus_tag="VLD60_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45361.1"
                     /translation="MAIRESDEISDKPAQGKSDSYSADQIKVLEGLDAVRKRPAMYIG
                     STGVDGLHHLVYEVVDNSVDEHMAGFGESIDVTIHIDGSVTVVDNGRGIPTGMHPTQK
                     KSAAEVALTVLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWLELEIWQDGQVFEQRY
                     QRGKPDAPLKDTGKTKRRGTKVRFKPDGQVFETLEFSFDVLAQRLRELAFLNKGLSIT
                     LKDERKEPAKEQVFFYKGGIVSFVEHLNEAKTPLHKPIYVNVEKPEMILEVALQYNDS
                     YAENLFSFANNINTKEGGTHLVGFKAALTRTINNYANANDLLKKETESLTGDDVREGL
                     TAVVSVKLRNPQFEGQTKAKLGNSEVKGVV"
     gene            complement(1171..2298)
                     /gene="dnaN"
                     /locus_tag="VLD60_10140"
     CDS             complement(1171..2298)
                     /gene="dnaN"
                     /locus_tag="VLD60_10140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSB45362.1"
                     /translation="MKVRIGRDELLTGLQRVQGVVEKRNTMPILSNILIEAKQEGAEI
                     VATDLEIGMRGLYKATVQKPGGITVSARKLYEIIKELPSGEIEINSGDNNWTTIQAGK
                     SQFKIVGLPSADYPALPAIEREGLTPLSGAGLLELIRKTLFAAGDNDARYILNGLLVT
                     LITSEKKTTLRLVGTDGHRLAVAEQDLGKAGAKGAPQEIKAIIPKKAAHEMQRLLQEG
                     GDGEPLIGFTKNLMIFRKSGLLLTSRLMEGNYPNYQQVIPKESNKKIIINRLDLESAL
                     RRVSVLSKDKANAVKLSFAPGRMTLFSSNPDYGEATEEMSAQYEGEALNTGFNARYLL
                     DVFGVMDGEKISLQMDSALSPCLIQEAETPGFKCVVMPIKI"
     gene            complement(2324..3667)
                     /gene="dnaA"
                     /locus_tag="VLD60_10145"
     CDS             complement(2324..3667)
                     /gene="dnaA"
                     /locus_tag="VLD60_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSB45363.1"
                     /translation="MTAATVWQEALAYIQGKVPKQVYDTWFTPVRLERIEDSTAQIGV
                     PNKFFGEWLGQHYKPLLEEAVSTARGGGETAVSFVVFNRHPSSQTDPATLVQAVKTAT
                     ATKPRRGIQLNPKYTFKNFVVGAGNQFAHAACMAVAEQPAKAYNPLFIYGGVGLGKTH
                     LLNAIANHVAERTDLRIAYLTTEQFTNEVINSIRYDKMMDLRKRYRHIDMLMIDDIQF
                     LAGKERTQEEFFHTFNALYEAHKQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPP
                     DVETRIAILRKKSEDEGVTLPEDVIQFLSTTMKSNIRELEGSLVRLGAYASLTGQTIT
                     LDMAKNVLRDLIGDKKKVVSMEDIQEIVGTWFHVKIADLKSRRRSKTLVHPRQIAMYL
                     CRELTDASYPEIGRLFGGKDHTTIIHACRQITKARENDSALHATLDGLKEKILRS"
     gene            complement(4230..4574)
                     /locus_tag="VLD60_10150"
     CDS             complement(4230..4574)
                     /locus_tag="VLD60_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45364.1"
                     /translation="MSKRVAVVISEDPCVTHRPVEALRIALGLCAGDHDTTVVLLGRA
                     PLLLTDDIDDVVDVEILEKYRPSFAQLEVPFVLESDSSIPAVRGEFTVSRSKPDDIRT
                     FLQSVDRTLVFS"
     gene            complement(4607..4927)
                     /locus_tag="VLD60_10155"
     CDS             complement(4607..4927)
                     /locus_tag="VLD60_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSB45365.1"
                     /translation="MASRKLGLLLSTAPSHSSVDTVVQLAEAAIRQGVEVYLYLIDEG
                     VKSMQDLRFTSLLDGGVRMSVCAYGCQQHRVSTVNVDSRISLSGLVVLSGIIDGCDRF
                     LAFT"
     gene            complement(5045..6301)
                     /gene="hisS"
                     /locus_tag="VLD60_10160"
     CDS             complement(5045..6301)
                     /gene="hisS"
                     /locus_tag="VLD60_10160"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSB45366.1"
                     /translation="MTIKGIKGVKDILPEETPRWRFIEERARRWADRYGFQEVRIPIF
                     EVTALFARSIGASTDIVEKEMYTFPDRDGTSLTLRPEGTAGTVRAFVEHNRAADPLPQ
                     KYYYLGPMFRHERPQAGRLRQFHQFGVEYFGTQDPRADVEVIALLWRLLSDLALPGLT
                     LEMNSLGSSEDRAAYLPLLVAFLKERTARLCANCQRRMESNPLRVLDCKIPECRAATE
                     DAPHLSSHLSPDAKNHFDHVLGGLTALGIPYQLNPRLVRGLDYYCLTAFEITSTHLGA
                     QNAVGAGGRYDGLVETLGGPKTPAVGFAVGLERISLMLPEETLPPAPPSVYVAAFGVE
                     GGRVGVLLLEELRRAGITAQSDYRANNLKAHLRQADRAHCRYAILLGDDEATRGSVIL
                     RNMDSKVQEEIRLPEIPAHLRSIIAQ"
     gene            6467..7954
                     /locus_tag="VLD60_10165"
     CDS             6467..7954
                     /locus_tag="VLD60_10165"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSB45367.1"
                     /translation="MSADRPTRRVIAVAPMPPEKSAYALARYSRSPDSIEHSIKWVHG
                     HSSEKFWDQFYFDYGHASIADLGHVIICFEEITELAAIRLEDEPVWDGQAKSSRYQNF
                     ASSSWYVPDAIHGSETEGTYQGILRSLGEVYRLLHEPLTKFLSDREPRPESMKPADYQ
                     RTIAARAFDVTRYLLPLASRTNVGQVVSIRTLEKQITRLLSSQLPELRMIGEDLKDAC
                     QRPPMNVWGELSGQTAGLNEPMAPTLARHAKANEYQTSVYTDLARYAKEALRGTGLDQ
                     PSTWGIQELVELIEPHDPLDETVATLLYRVSQAPYRKILSVVRDWSEKQKQDTLAVAF
                     SNRGPYDELIKEFRSGYAFTFDVLMDIGGWRDMHRHRRCQQIQQNFTTVHGYDVPPPL
                     VQAGLDGEYRQAMDAVRADIEQLRKVNAEAGLYAIPFGFKVRCLFKMDFAEAEYIAKL
                     RSGVKGHWSYRTIAWLMKEKLAERYPALGAHMQATSPDIEDTLTR"
     gene            7977..9014
                     /locus_tag="VLD60_10170"
     CDS             7977..9014
                     /locus_tag="VLD60_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45368.1"
                     /translation="MSEHQIQCEAALLAGEWEGAAQAALAWARHPASTDQLDPRPHFV
                     LNVVHLIKGQFAEAWAAHAKCLHEPDDIAQVKSWIEGLVAGHAEQAAGHLVMGLFLAQ
                     SGQSEQSMQSYKESAKQAPQWAYPHYFLAQIHERANHVEMAIKEYREAVRLDPSYAPA
                     RTNLGVAYQEQGRLEMAIPQYREVIKLQPNDAVAHANLACALAEQGKMEPAVQAYKES
                     LRLNPSDAEVHFALGSLYETRNRLDLAQREYDEALRANPDFGPAHTALGWLSLGKGRL
                     QSAFEAFNRGLKANAQDARAYHGIAEYYAQIKKPESALDNYTKALKYYQDPDKKNMIM
                     NQLFQEGRVPD"
     gene            complement(9062..9331)
                     /locus_tag="VLD60_10175"
     CDS             complement(9062..9331)
                     /locus_tag="VLD60_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45369.1"
                     /translation="MKGLRFERIGQGRYYNVVFHLGSTYVPVSDETVEELKAQSLLPA
                     ERFLDFLIDRVGYSSYLKEQIRTELKSSGDPVTQITVLQGAIREL"
     gene            complement(9415..9756)
                     /locus_tag="VLD60_10180"
     CDS             complement(9415..9756)
                     /locus_tag="VLD60_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSB45370.1"
                     /translation="MGGLILHPMKEIRVIVAGEHRAFVTDLLDRVNATGYTIIGNLSG
                     KGHHGVREAHFMFSEQESLVMIMAVVPETKVEPVLAGLRPLFERHSGVMFVSDVSVSR
                     QEYFGKEHGGS"
     gene            complement(9780..12995)
                     /locus_tag="VLD60_10185"
     CDS             complement(9780..12995)
                     /locus_tag="VLD60_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HSB45371.1"
                     /translation="MPSPDLETRRMELRGIVKLAGEVIAQYWPMRTFVHHNPLHSLEY
                     LPFEETVRRGKQFMGGDGYLPGWMYRAYLKSGRIQSRHLEEALQPLAQDKQVTIGSRT
                     VMHHEVLRACLTEGLCSPILEPLEHHLPDPSGELIDRVATRLKSVMTFPDLHERIRAI
                     IDGDQAALGRWLTLSHWCDDTFGTQIVRQINDQLIKWCEAFLDEGHATWAMQEREQGL
                     YQAWKAIAAHEWSPCGIADSQRKIAQLPDHPEDALLESLDALGIPSKLRQDYLSLQLT
                     ALPGWAGFIKWRGEERDYPWQRAYPVGLVKFLAIRLWYARELVQKACREELGIEGRYD
                     AVTSYMRHHAGEYYLRRQRVAGRLPALYAEEVDRLAHRKSNGWKAVLDRYQTEVVPRQ
                     LAAARRGAARKLLALARSLELDPAQLVAAAPEHLKQMADWIDSFPESDHGPVWLKAFE
                     AGYHERLLGQLRTRTAEADRPAATRPYSQSVYCIDVRSEPFRRHLESVGPHETYGFAG
                     FFAAFIRYRAWGKEHDTEQFPVIMRAKNEVREIPRSYLDHKVSKHEARAKWVHAGHTL
                     LHDLKENVVTPYVMVESLGWFYSLPIFGKTLLPSLYQRWTAWLKRLFVPGLATTLTVD
                     KLPPAETAEMLVGEQQAIVRKALQDRLGLRSSRITPALVEALRLQALNGQEDLSALRD
                     AASEAGITPEKLTVFVEILRRQYDLNARAASHHKERITRTGFTVDEQTLTVDTALRMM
                     GLTKNFARLVLFCAHGSTSENNPYESALDCGACGGNEGKPNARTLAMMANNPKVRERI
                     AKMGIEIPSDTHFLAGQMNTTTDVVELFDLEDVPPTHRADLARLQEDLKEASLLTSRE
                     RCARFPDVHQVLEETKAEAHVRRRSVDWSQVRPEWGLSSNASFLIGRRELTKGLDLGG
                     RVFLHSYDYREDPSNRLLEVLLTAPQVVAQWINMEHYFSAVDNEVYGSGSKIYHNVVG
                     RIGIMSGPWSDLRLGLARQTMMNGDVPYHEPMRLLTVVEAPRGRIEKLIARHEVLQHF
                     YHNEWVHLVALDPEDRVWYRYRSNGEWSPVSDPPQQEHIHM"
     gene            complement(12995..14305)
                     /locus_tag="VLD60_10190"
     CDS             complement(12995..14305)
                     /locus_tag="VLD60_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSB45372.1"
                     /translation="MKDGALTAWLLAGIPLLGAAGSLAFWSKPVRLKQSSIAWSFVSL
                     GSIVALSGQVGMLPEGLLPLYLLPLTAMISLLGQPAHADHRESYIMTLLMLGLGVCVL
                     AHQPPVAAVALLLLFLLMAGLLYRHHSALWPMSWWGIGSYAIGVLCAGFSLGADAPLS
                     SVASLVASAVLLPLMPFHDGHLTAVTRFPGNLPSFVVLLLPLLGLHGIAMALPTIPDT
                     VAWTLSLFALVGAFYGAVKALAQTRVRLLLAYGSLSFFSMLWWFAAASRSATPRSALL
                     VGTVGLATSGLLVAWQVVRTRYGDDVDPQAISGLAWGMPQYAVLLSLLALAAMGLPPF
                     GVFAGFMGLLLSSPFPSSGGLVVTLLAWLAASWYIMQMVQRLLFGLRRPELRYVDVLR
                     TEFVALLLVVLVLLGLGLAPSTLFVPDHTMAAIHSVLGPVVWNR"
     gene            complement(14302..>15021)
                     /locus_tag="VLD60_10195"
     CDS             complement(14302..>15021)
                     /locus_tag="VLD60_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSB45373.1"
                     /translation="GAFSLAVFHLIAHGLFKATVFLNCGNVIHKARQEPHFPHVDHQP
                     DEEGLSRLTWFTGFITTLLIPLLILLVTHGILRIPLLESQGTVIFLFFIWITSSQAIL
                     TLTRLRQVASWKVSAAMLITLLFVVFIYLGAAEAFTAFLYPNPEEVASYFEAAALPNW
                     IFDLILITATALTIASWSYLYMTAHGRSVRMPAWIERLRIRLYVPFMNRLYADELYQW
                     LGNSIMRLVHQLDKLERGWSR"
BASE COUNT         3200 a         4442 c         4348 g         3031 t
ORIGIN      
        1 acgacgcctt tgacttcgct gtttccgagc ttcgccttcg tctgtccctc gaactgcgga
       61 ttgcggagct tcacgctgac gaccgccgtg agcccttctc ggacgtcgtc gccggtgagc
      121 gactcggttt ccttctttaa caggtcgttt gcattcgcgt agttgttgat ggtccgcgtc
      181 aatgcggcct taaatcccac caggtgcgtg ccgccttcct tcgtgttgat gttgttcgca
      241 aaggagaaga gattttccgc gtagctgtcg ttgtactgga gcgcgacctc gagaatcatc
      301 tccggttttt cgacgttcac gtagatcggc ttgtgcagcg gtgttttcgc ttcgttcaaa
      361 tgctcgacga aggagacgat gccgcccttg tagaagaaca cctgctcttt ggccggttcc
      421 tttcgttcgt ccttcagggt gatcgagagg cctttgttta agaaggcgag ttcgcgtagc
      481 cgttgtgcca gcacgtcaaa gctaaactcc agcgtctcga acacttgccc gtccggcttg
      541 aatctcacct tcgtgccgcg acgcttcgtc tttccggtgt ccttcagcgg tgcgtccggc
      601 ttccctcgct ggtagcgctg ctcgaacacc tggccgtcct gccagatttc gagctccagc
      661 cattccgaga gcgcgttcac caccgagatg ccgacaccgt ggaggccgcc cgacacggta
      721 tacgctccct gctcgaactt cccgcccgcg tgcaggacgg tcagcgccac ttcggcagcg
      781 gatttctttt gcgtcggatg catgccggtg ggaatgccgc ggccgttatc gaccaccgtc
      841 acgctgccgt cgatatggat ggtgacgtcg atcgattcgc cgaagccggc catgtgttcg
      901 tcaacgctgt tgtcgacgac ttcgtaaacc aaatggtgca ggccgtccac gccggtactg
      961 ccgatataca tcgcaggccg cttgcgaacg gcatcgagac cctcgaggac tttgatttga
     1021 tccgcgctgt agctgtcgga ttttccctgg gcgggcttgt cggaaatctc gtcggactct
     1081 cttatcgcca ttcgtctcct tcggtgcagc tattgccgtt cggcaatcag cgtcgaggtg
     1141 atgccgtgag gctgactgct cgcttctgcc tcaaatctta atcggcatca cgacgcattt
     1201 gaatccgggc gtctcggctt cttggatcaa gcagggactc aaggcactgt ccatttgcag
     1261 agaaatcttc tccccgtcca tgactccgaa gacatccagc aagtatcgag cgttaaatcc
     1321 ggtgttgagc gcttcaccct cgtactgcgc cgacatctcc tcagtggctt ccccgtagtc
     1381 cggattgctg gagaacaggg tcatgcgccc cggagcgaac gacagcttca cggcattcgc
     1441 cttgtccttc gacagcaccg aaacccgccg tagcgcgctt tccaggtcca ggcgattgat
     1501 aatgatcttt ttgttgctct ctttgggaat cacctgttgg taatttgggt agttcccttc
     1561 catcagacga gaggtcagga gcaagccgct cttgcgaaag atcatcaggt tcttcgtaaa
     1621 tccgatcaag ggctcgccgt cgcccccctc ttgcaacagc cgctgcattt catgggcggc
     1681 cttcttggga ataatggctt tgatttcctg gggggcgccc ttggctcccg ccttgccgag
     1741 gtcttgttca gccacggcca atcgatggcc atcggtcccg accaggcgga gcgtggtctt
     1801 cttctcgctc gtgatcagcg tgaccagcaa gccgttcagg atgtagcgcg cgtcattgtc
     1861 gcccgccgca aagagagtct ttcggatcaa ttcgaggagg cccgcaccgg agagaggcgt
     1921 taatccttcg cgctcgatcg ccggcagtgc gggataatcc gcgctcggca ggcccacgat
     1981 tttgaactgg ctcttgccag cttgaatcgt cgtccagttg ttgtcgccgg aattgatctc
     2041 gatctcaccg ctcggcagtt ccttgatgat ttcgtagagc ttgcgcgccg acaccgtaat
     2101 gccgcccggc ttctgcacgg tggctttgta gagcccgcgc attccgatct cgagatcggt
     2161 cgcgacgatc tccgcccctt cctgtttcgc ctcgatcaag atgttcgaga gaatgggcat
     2221 cgtattgcgt ttttcgacga cgccttgcac ccgttgcagg ccggtcaaca gctcatctcg
     2281 tccgattcga accttcatga ttccctctcc ctcgaaccac cgttcagctt cgaaggatct
     2341 tttccttcaa gccgtccaac gtcgcgtgca atgcgctgtc gttttccctg gccttcgtaa
     2401 tctgccggca ggcatgaatg atcgtcgtat ggtcctttcc cccgaagagt cgaccgattt
     2461 ctgggtagga cgcatccgtg agttcgcgac agaggtacat agcgatctgc ctggggtgca
     2521 cgagcgtctt gctccggcgc cttgatttca gatccgcgat cttcacgtga aaccaggtgc
     2581 cgacgatttc ctggatgtct tccatcgaca caaccttttt cttgtcgccg ataaggtctc
     2641 ggagcacgtt cttggccatg tcgagcgtga tcgtctgccc agtcagcgag gcgtaggcgc
     2701 cgagccggac cagcgaaccc tcgagctctc gaatattgct tttcatggtc gtcgataaaa
     2761 actggatgac gtcttcgggt aacgtgacgc cttcgtcctc tgatttcttg cgaaggatcg
     2821 cgattcttgt ctcgacgtcc ggaggctgaa gatcggcgat caacccccat tcgaatcgag
     2881 acctcagccg ttcctcgata tctggcatgt ccttcgggaa acggtcgctt gaaaggacga
     2941 tctgcttgtg cgcttcgtag agggcattga aggtatggaa gaattcttcc tgtgtccgct
     3001 ccttgcccgc caagaactga atatcgtcga tcatcagcat gtcgatgtga cgatagcgct
     3061 ttctgaggtc catcatcttg tcgtaccgaa tcgagttgat gacctcattg gtaaactgtt
     3121 cggtcgtgag ataggcgatc cggaggtcgg tccgctccgc cacatggttg gcaatggcat
     3181 tcaacaggtg tgtcttgccc agaccgacgc cgccgtagat aaagagaggg ttataggctt
     3241 ttgccggctg ctccgccacg gccatgcagg ctgcgtgggc aaactggttc cctgccccga
     3301 ccacaaaatt cttgaacgtg tactttgggt taagttggat gccccttctc ggctttgtcg
     3361 ccgtagcggt cttcacggct tggacgagag tggcagggtc ggtctggctg ctgggatgcc
     3421 ggttaaagac gacaaaggag acagccgttt ctcccccgcc tcgagcggtc gatacagcct
     3481 cttccaggag gggcttgtag tgctgtccta accactcacc aaagaactta ttcggcaccc
     3541 cgatttgggc cgttgagtct tcaattcgct caaggcgaac aggcgtaaac cacgtgtcat
     3601 acacctgttt tgggactttg ccctggatat acgccaaagc ctcttgccag acagttgcgg
     3661 ccgtcataat acccctatta ttccccatac acaggcttat tcacacctgt ggataaccct
     3721 tatcctgacc agccctcttt ccttaatccg ccatccctga ccgagtcatg agatcctcct
     3781 cacatactcg gtcgtatcgc accccagaac ttgtgaactc tgtaccctcc ataaacccag
     3841 agtcaatccc caagttcatc cccatggtga cgatagaaat tctgatttcc gatcgatcgg
     3901 ttatctccct tccccactat tcacatgact tactactact tctcctacga cgactatctt
     3961 cttcattcac acaatctctt cggagaagag ggatcaatat cagatcacga taacgtgctc
     4021 gctctcaaac atcttctcaa tgagatcatc gtatgaaatg tgtggatgcc gaccattctt
     4081 ctctacagaa aaaacatggc ctttcgagtg tgacaactcg gaacaagcag cttctccaaa
     4141 gagaacaaca tcaccttcag tttcggagga aagaaacatt gctcgattca cttgctcaac
     4201 cggttgccga agcaggtata aagtcttgct catgaaaaca ccagtgttcg atccacagac
     4261 tgaagaaaag tacgaatatc atcaggtttt gaacgggaaa cggtgaattc tcctcgaaca
     4321 gcagggatgg atgaatcaga ttccagtaca aagggaactt caagttgggc aaaggagggc
     4381 cgatattttt caagaatctc aacatcgacg acgtcgtcaa tatcatcagt caagaggagc
     4441 ggagcacgcc caaggagaac caccgtcgtg tcatgatcgc cagcacagag cccaagggcg
     4501 atacgcaaag cctcgacggg gcgatgggtc acgcaaggat cttctgaaat gacaacagca
     4561 acgcgcttag acatagaaac ctgattgaaa acacgagtct actcgactac gtgaacgcca
     4621 aaaatcgatc acagccatcg atgattccgg ataaaacaac aaggccggag agcgatattc
     4681 ttgaatcaac atttacagta gatactcgat gttgctggca tccatacgcg cacacggaca
     4741 tcctcacacc tccatccaac aaagatgtga agcgaagatc ttgcatactc ttcactccct
     4801 catcgatgag gtagaggtag acttcaaccc cctgccgaat cgccgcttcc gcgagttgca
     4861 ccactgtgtc gacactagag tgagaagggg ctgtggataa caagagaccg agtttgcgag
     4921 atgccatgaa aataccagga gaatgaaaag gaagttcttt taataatcaa atggttacat
     4981 tatccattcg aggcgagcgt acggggtttt tttgaggaag tcaatacgaa aaaagaggcc
     5041 cttattactg ggcaataatg gagcgcagat gagcggggat ttccggtagg cgaatttctt
     5101 cctggacctt cgaatccata ttccgaagga taacggaacc tcgtgtcgct tcatcgtcac
     5161 caaggaggat ggcataacga caatgggccc ggtcggcttg gcggagatgt gccttgaggt
     5221 tattggcccg ataatcggat tgggctgtaa tcccggcacg acgaagttct tcaaggagca
     5281 gaactcccac ccgcccgcct tcgactccga acgcagcaac gtacaccgat ggaggggccg
     5341 gagggagggt ctcttccggc agcatgaggg agattcgctc gagcccgacc gcaaacccga
     5401 cagccggcgt ttttggccca ccgagcgtct cgacgagccc atcataacgt ccaccggccc
     5461 caacggcatt ctgtgcacca aggtgcgtgg aggtaatttc aaatgccgtg aggcaatagt
     5521 agtcaagccc tcgcacgagc ctcggattca gttgataggg gatgccgagt gctgtcagac
     5581 cgccgaggac atggtcaaaa tggtttttcg catcgggcga cagatgagag ctcaggtgcg
     5641 gagcatcttc ggttgccgct cgacattctg gaatcttgca gtcgagcact cgaagcggat
     5701 tgctttccat tcggcgttgg caattggcac agagacgagc ggttcgctct ttgagaaacg
     5761 cgacgagaag gggcaggtag gccgcccgat cctccgagct cccaagactg ttcatttcga
     5821 gcgtcaaacc cggaagcgcg agatccgata agagacgcca gagtaaggcg atgacttcca
     5881 catcagcacg ggggtcctgc gtcccgaaat actcaacgcc gaattgatgg aactgcctga
     5941 gccggccggc ttgggggcgt tcatgtcgga acatcgggcc aaggtagtaa tatttttgag
     6001 gcaggggatc ggcggcacga ttatgttcga cgaaggctcg aacggttcct gcagttccct
     6061 ccggccggag cgtcagcgag gtgccgtctc gatcgggaaa ggtatacatt tctttttcga
     6121 cgatatccgt cgacgcgccg atactgcgcg caaaaagcgc cgtcacttcg aaaatgggaa
     6181 tccggacctc ttggaagccg taccgatccg cccagcgcct ggctcgctcc tcgataaacc
     6241 gccagcgggg cgtttcctcc gggaggatgt ctttcacgcc tttgatgccc ttgatcgtca
     6301 tcgtctcatc acccaagaga ttcaaaaaga ttcgggaata tagcggcggc attgcgggca
     6361 agtcaacgcg atctcgggcc cgacgcagat gcttgcacag accggagcta ggaggctata
     6421 gttcgcactc cccggttgtc gacttgattc acgcgggaga gatgttgtga gtgccgatcg
     6481 accgacccga cgcgtcatcg ccgtggcacc gatgccgccc gagaaatccg cctacgcgct
     6541 cgcccgctac agccgctcgc ccgactcgat cgaacacagc atcaagtggg tgcacggcca
     6601 ttcgtcggag aagttctggg accaattcta ttttgattac gggcacgcct cgatcgccga
     6661 cctcggccac gtgatcatct gtttcgagga aatcaccgaa ctggctgcca tcaggctgga
     6721 agacgaaccg gtgtgggacg ggcaggccaa gtcgagccgc tatcagaact ttgcctccag
     6781 cagctggtat gtcccggacg ccattcacgg gagcgaaacg gaagggacct accaagggat
     6841 tctccgcagc ctcggcgaag tgtatcggct gctgcacgaa ccactgacga agtttctctc
     6901 cgatcgcgaa ccgaggccgg aatccatgaa accggccgat tatcagcgga cgatcgccgc
     6961 ccgggcgttc gacgtcacgc gctatttgct cccgcttgcc tcgcggacga acgtcggaca
     7021 ggtcgtcagc attcggacgc tggaaaaaca gattacccgc ctgctttcat cgcaactgcc
     7081 cgagcttcgg atgatcggcg aggacctcaa agatgcctgc cagcggcctc cgatgaatgt
     7141 gtggggagaa ctgagcgggc aaacagccgg gctcaatgaa ccgatggccc cgacgcttgc
     7201 cagacacgca aaagcgaacg agtaccagac gagcgtgtac acggacctcg cacgctacgc
     7261 caaggaggcc ctgcgcggaa ccggcctcga tcagccatcg acctggggaa tccaagagct
     7321 ggtcgaacta attgagccgc acgatccatt ggatgaaacc gtcgccacgc tcctataccg
     7381 cgtgtcccaa gcgccctatc ggaagattct cagcgttgtg cgcgactggt cggagaaaca
     7441 aaagcaggac acgcttgcgg ttgcgttcag caatcggggc ccctatgacg aactcatcaa
     7501 ggaattccgg agcggctacg ccttcacctt cgatgtgctg atggatatcg gcggctggcg
     7561 cgacatgcat cggcaccggc gctgccagca aatccagcag aacttcacga cggtgcatgg
     7621 ctacgatgtg ccgccgccgc tcgtgcaggc tggactcgac ggggagtatc ggcaggcgat
     7681 ggacgccgtg cgagccgaca tcgagcagtt aagaaaggtc aatgcggaag cgggcctcta
     7741 tgcgatcccc ttcgggttca aggttcgctg cctcttcaaa atggactttg ccgaagccga
     7801 atacatcgca aaactgcgtt cgggcgtgaa aggccactgg tcctaccgca cgattgcctg
     7861 gctgatgaag gagaaactgg ccgagcgcta ccccgccctc ggagcacaca tgcaggcaac
     7921 ctcgccggac atcgaagaca ccctgacgcg gtagcggtca tcgatcacac gcacccatga
     7981 gcgagcacca aatacaatgc gaagccgcct tattggccgg tgagtgggaa ggggcagccc
     8041 aagccgccct ggcctgggcg cgccatcctg cctcgaccga ccagctcgac ccccgtcccc
     8101 atttcgtcct gaacgtcgtc cacctcatta aggggcagtt tgccgaagct tgggctgctc
     8161 atgccaagtg tttgcacgaa ccggatgaca ttgcgcaggt gaaaagctgg atcgaaggac
     8221 tagtggctgg ccatgctgaa caggcagcgg ggcacctggt gatggggctg ttcctggcgc
     8281 aatcaggcca gtcagaacag tccatgcagt cctataagga gtctgctaag caggcaccgc
     8341 agtgggccta cccgcactac tttcttgcac agattcacga acgggcgaac catgtggaaa
     8401 tggcgatcaa agaataccgg gaggcggtgc gactcgatcc gtcttacgca ccagcgcgca
     8461 cgaatctcgg cgtggcttac caggaacaag ggcgactcga aatggcgatc ccgcagtatc
     8521 gggaagtgat caagttacag ccgaacgacg ccgttgctca cgcgaatctc gcttgtgcgc
     8581 tcgccgagca gggcaagatg gaaccggccg tgcaggccta caaggaatca ttgcggttga
     8641 atccgagcga tgccgaagtg cacttcgcgc tcgggagcct ctatgaaacg cgcaaccgcc
     8701 tggatctcgc ccagagggag tacgacgaag ccctgcgcgc caatcccgac ttcggtccgg
     8761 cacatacggc gctgggatgg ttgtcgctgg gcaaggggcg cctgcagagc gccttcgagg
     8821 ccttcaatcg cgggctcaaa gccaacgccc aggacgcgcg tgcctatcac ggcattgccg
     8881 agtactacgc gcaaatcaag aagcccgaga gcgcgctgga caattacacg aaggcgctca
     8941 agtactacca ggaccccgac aagaagaaca tgatcatgaa tcagctgttt caggaaggac
     9001 gagtgccgga ctgaggaact gagaacacgg cattctcacc ggaaagaaaa cgaacttaag
     9061 atcagagctc tctgatagca ccctggagca cggtaatctg cgtcaccgga tcgcccgagc
     9121 tcttcagttc ggtgcggatt tgctccttga ggtaggatga atagccgacg cggtcgatga
     9181 ggaagtccag gaaccgttcg gccggcagga ggctctgcgc ttttagttcc tccacggttt
     9241 catcgctcac gggcacgtag gtgctgccga gatggaacac tacgttgtaa taacgccctt
     9301 gcccgatgcg ctcgaatcgt aagcccttca cctcacccct ccatcgtgaa accgctcaag
     9361 aggggcccat tctagacaat cgaccaccca gaagacaagg gaggcgctcc gcgatcatga
     9421 cccgccgtgt tccttgccga aatactcctg tcgactgacc gaaacgtcgc tgacgaacat
     9481 gacccccgaa tggcgttcaa acagtggccg cagtcctgcc aggactggtt ccactttggt
     9541 ctcggggacc accgccatga tcatcaccag gctctcctgc tcgctgaaca tgaagtgggc
     9601 ctcgcgcacc ccatggtgcc cctttccgga caggttgccg atgatcgtgt agccggtggc
     9661 attgacgcgg tccagcaagt cagtcacgaa cgcccggtgt tctcccgcaa cgatcacgcg
     9721 aatctccttc atcggatgca aaatcaaacc gcccatcgat acgctccttt cacggcctgc
     9781 tacatgtgaa tgtgttcctg ctgtggggga tcgctcacgg ggctccactc gccgttggac
     9841 cggtaccggt accatacccg atcttcgggg tcgagagcga cgagatggac ccactcgttg
     9901 tggtaaaagt gctgcaagac ttcatgccgc gcgatcagtt tctcgatgcg gccgcgcggc
     9961 gcctccacaa cggtcagcaa ccgcatcggt tcatggtacg gcacgtcgcc gttcatcatc
    10021 gtctgccggg cgaggccaag ccgcagatcg ctccagggtc cggacatgat gccgattcgc
    10081 ccgacgacat tgtgatagat cttgctgccg ctcccgtaca cctcgttgtc gacggcggaa
    10141 aagtaatgtt ccatgttgat ccactgcgcc acgacctggg gcgcggtgag cagcacttcg
    10201 agcagacgat tgctcgggtc ttcgcgatag tcgtacgagt gcaggaagac gcgccctccg
    10261 aggtccaatc ctttggttag ctcccgccgt ccgatcagga acgaggcgtt gctcgataga
    10321 ccccactcag gacgcacctg gctccagtcg acgcttcgcc gtctgacgtg cgcctcagcc
    10381 ttggtctctt caaggacctg gtggacgtcg ggaaagcgcg cacagcgttc ccggctggtg
    10441 agcagcgacg cctccttgag gtcttcttgc aggcgcgcca gatccgcccg atgggtcggc
    10501 ggcacatctt ccaagtcgaa gagctccacg acgtcggtcg tcgtattcat ctggccggcg
    10561 agaaaatgcg tgtcggaggg gatctcgatg cccattttcg cgatgcgttc gcggactttg
    10621 ggattgttcg ccatcatcgc tagcgtgcgc gcgttgggct tgccttcgtt gccgccgcag
    10681 gcgccgcaat cgagcgccga ctcgtacggg ttgttctccg acgtgctgcc gtgcgcgcag
    10741 aacagcacga ggcgggcgaa gttcttggtc agccccatca tgcgtaaggc cgtgtccacc
    10801 gtgagcgtct gctcgtcgac ggtaaagccg gtccgcgtga tccgttcctt gtgatgcgag
    10861 gcggcccgcg cattgaggtc gtactgccgg cgcaagattt ccacaaacac cgtcagcttc
    10921 tccggcgtga tcccggcctc agaagccgcg tctcgcaacg ccgacagatc ctcttgcccg
    10981 ttcaaggcct gcagtcggag cgcttcgacg agtgcgggcg tgatccgcga gctgcgtaac
    11041 cccagccggt cctgcagcgc ttttctgacg attgcctgct gctcccccac caacatctcc
    11101 gccgtctcgg ccggcggcag cttgtccacg gtcaaggtgg tggcgagccc cggcacgaag
    11161 agtcgtttca gccacgcggt ccaacgctga tagagcgagg gcaggagggt cttcccgaag
    11221 ataggaagac tgtagaacca tccgagcgac tcgaccatga catagggtgt caccacgttt
    11281 tctttcagat cgtgcaacag cgtgtgtccg gcgtggaccc atttggcgcg ggcttcgtgt
    11341 ttcgagacct tgtggtcgag gtagcttctt ggaatctccc gcacctcgtt cttcgcccgc
    11401 atgatcaccg ggaactgttc agtatcgtgt tctttgcccc aggcccgata gcggatgaag
    11461 gcggcgaaga agccggcaaa gccgtatgtt tcgtgcggac cgaccgattc gagatggcga
    11521 cggaacggtt cggagcgcac gtcgatgcaa tagacggatt gcgaataggg acgggttgcg
    11581 gccggccgat cggcctcggc cgttcgcgtg cgcagttgtc ccagcaggcg ctcgtgatag
    11641 ccggcttcaa aggctttcaa ccagactggg ccatgatccg actccgggaa cgagtcgatc
    11701 caatcggcca tttgcttgag gtgctccggg gccgccgcca cgagctgtgc cggatcgagc
    11761 tccaaggatc gggccagcgc gaggagcttg cgtgccgcgc cgcgccgcgc ggccgccagc
    11821 tgccgaggca ccacttccgt ctggtagcgg tcgagcacgg ccttccagcc gttgcttttt
    11881 cgatgcgcga gccggtcaac ttcttcagcg tagagggcgg ggaggcgtcc ggcaactcgt
    11941 tgccgccgca agtaatactc tcctgcatgg tggcgcatgt acgatgtaac ggcatcgtag
    12001 cgcccctcga tgccgagttc ttcgcggcag gccttctgca ccagctcgcg cgcataccaa
    12061 agccgaatgg cgaggaactt caccaggccg acgggatagg cccgttgcca gggatagtcc
    12121 cgttcttccc cgcgccactt gatgaaacca gcccagccgg gcagggccgt caactgcagc
    12181 gacaagtaat cctgccgcag tttggacgga atgccgagag catctagact ctcgagaagc
    12241 gcgtcttccg gatggtcggg gagctgcgcg atcttgcgtt ggctgtcggc gatcccgcag
    12301 ggtgaccatt cgtgcgcggc gatcgccttc cacgcctggt acaggccctg ctcgcgttcc
    12361 tgcatggccc atgtcgcatg gccttcgtcg agaaacgcct cgcaccactt gatcagttga
    12421 tcgttgatct gccgaacgat ctgtgtgccg aaggtgtcgt cgcaccagtg cgagagtgtg
    12481 agccaacggc cgagcgccgc ctgatcgccg tctatgatgg cgcgaatacg ttcgtgcaga
    12541 tccggaaagg tcatgacgga tttgagccgg gtggcgacac gatcgatcag ctcacccgag
    12601 ggatccggca ggtggtgctc aagtggctcg aggatgggcg aacataaacc ttcggtcagg
    12661 caggcgcgca agacctcatg gtgcatgacg gtacgcgagc cgatggtgac ctgcttgtcc
    12721 tgcgcaaggg gctgcagggc ttcttcgaga tgacgggact ggatccggcc cgatttcaga
    12781 tacgctcggt acatccaccc ggggagatag ccgtcgccgc ccatgaactg cttgccgcga
    12841 cgaactgttt cttcgaatgg caggtattcc aggctatgga gcgggttgtg gtgcacgaag
    12901 gtccgcatcg gccagtactg cgcgatgact tcgccggcga gcttgacgat cccgcgcagc
    12961 tccatccgcc tggtttcaag gtcgggtgac ggcatcaccg gttccatacg acgggcccca
    13021 aaacggagtg aatggcagcc atggtgtgat caggcacaaa gagcgtggac ggtgccaagc
    13081 ccagtcccaa caggaccaac acgacgagaa ggagcgccac gaattcagta cggaggacgt
    13141 cgacatagcg caactcgggc cgccggagtc cgaagagcag gcgttgcacc atctgcatga
    13201 tgtaccaaga cgcggccagc caggcgagga gggtgacgac caatcctccc gatgacggaa
    13261 agggtgagct caacaacaat cccatgaatc ccgcaaacac accgaacggc ggcaggccca
    13321 ttgcggccaa agcgaggagc gagaggagga cggcgtattg aggcatgccc caagccaatc
    13381 cgctgatggc ctgcggatcc acgtcgtctc cgtatcgggt gcgaacgact tgccacgcga
    13441 ccaagagccc gctggtggcc aggccgaccg tcccgacgag cagcgcggac cgcggcgtcg
    13501 ccgatctgga agccgcggca aaccaccaga gcatcgaaaa gaacgaaagg ctgccgtacg
    13561 ccaacaataa ccgtacgcgt gtttgggcca gtgccttgac cgccccgtag aaggcgccca
    13621 ccaacgcaaa gaggctgagg gtccaagcga cggtgtcggg gatggtcggc agcgccatcg
    13681 cgatcccgtg caggccgagg agcgggagca agaggacgac gaacgacggc aggttgccag
    13741 gaaagcgcgt cacggccgtc aggtggccgt cgtggaacgg catgagcggc agcaacaccg
    13801 cggaggccac caaggacgcg acggaggaga ggggagcatc cgccccgagc gaaaagcccg
    13861 cgcacaagac cccgatggca tagctaccga tgccccacca ggacatgggc cagagcgcac
    13921 tgtgatgacg atagagcaag ccggccatca gcaagaagag cagtagcaag gctacggcgg
    13981 cgaccggcgg ttgatgggcc agcacgcaca cacccaagcc aagcatcagc aaggtcataa
    14041 tgtatgactc acgatgatcg gcatgagccg gctgtcccaa gagtgagatc atcgcagtga
    14101 gtgggagcaa gtagagcggc aagagtccct cagggagcat gccgacctgt ccggacaagg
    14161 ccacaatcga tccgagactg acaaaggacc aggcgatgga agattgtttc agccgaaccg
    14221 gctttgacca gaacgcgaga ctccccgcgg ctcccagcaa ggggatgccg gccaacaacc
    14281 atgcggtgag ggcgccgtcc ttcatcgcga ccaaccccgt tccaatttat cgagctgatg
    14341 aaccagccgc ataatgctgt ttcccagcca ctgatagagc tcatcggcgt acagccggtt
    14401 cataaagggc acgtagagac ggattcgtag tctttcgatc cacgccggca tgcgcacaga
    14461 tcggccgtga gccgtcatgt aaaggtagga ccaactggcg atcgtgaggg ccgtggctgt
    14521 gatgaggatg aggtcgaaga tccagttcgg cagggcggcc gcctcgaaat aggaggcgac
    14581 ttcctccgga ttcggataga gaaacgccgt gaaggcctcc gcagccccca ggtaaatgaa
    14641 cacgacaaac aacagcgtga tgagcatcgc cgccgaaacc ttccacgacg ccacctgccg
    14701 gagcctggtc agcgtcagga tggcttgcga ggacgtaatc cagatgaaga acaggaagat
    14761 gacggtccct tgggactcca agagagggat ccgcaggatt ccatgggtga ccaaaagaat
    14821 gagcaaggga atgaggaggg tcgtgatgaa tcccgtgaac caggtcagcc gggacaaccc
    14881 ctcttcgtcc ggctgatggt cgacgtgggg gaaatgcggt tcttgccggg ccttgtgaat
    14941 gacgttcccg caattcaaga atacagtcgc cttgaagagc ccgtgcgcga tcagatggaa
    15001 aacggccagc gagaaggccc c
//
LOCUS       DATIFR010000177         9886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2605509, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000177 DATIFR010000000
VERSION     DATIFR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605509"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3371
                     /locus_tag="VLD60_10200"
     CDS             <1..3371
                     /locus_tag="VLD60_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45374.1"
                     /translation="VTNIRNSQTQAYAPLAESLYEALCLYRKSQGPCYDNGTGTVWGT
                     NYTNSTGTTGDPFFFTSYNQMVRCCKNYVLMISPGIGVADGTNPTLQTPFPTGSTINP
                     TWTNNIGVKASTSAGDATSAAAGDRLDDVAYYGRTHDLRSDLTGSQYVTFYSVNAMGR
                     KVGATLLASAAKYGGFNDQNNDGTSNFNPTSGQTCNYPATSNLNPGTAGPFYSDPEWD
                     AHGPLPTDPPDCVPDTFFDANDGYGLRNQILAALDAILKNSASGTSISVLASSSNGDG
                     SIYQAYFYPETPKGDGTANVVSWTGYFQGLFIDTFGNLREDHGGASGAGDGKLVYTDD
                     NIVVTEVSPTTGEVVIRRYADADGDGKADDTGYYATPRTGGTVATLREMQGMWEAGKK
                     LALRDLSAKPRNLFTWVDLNDDGVVTANEQMAFSTANAATLSPYLRATSTGTFTATNI
                     ISFLQGNQVTGMRDRQFPVDGVQKTWRLGDIVNSSPTIVGPPRERFDILYGDNGYRQY
                     LQRWSNRRQTAYVGANDGMLHAFNLGYYHRADDPNTSALEHGWYTTAPSDNSSGPELG
                     EELWGFVPHYILPQLMWYTQSNYTHISYVDLKPKVTDVRIFTPEAACGSSASPTPTAT
                     GCIHPDGWGTILIMGMRFGGSCGICASVSGANNGGPPLAVTADFGSGVTTRYFYSGYI
                     VLDITDPDATPKVLSAFSNWGLGLTTSYPTVVRMSPKADGKNDHTNARWMMLLGSGVH
                     GYDGRAAMYGSMFGVLLVQQGTSPSYVQMPMLDQASSPLTYKSSYMSDLITFDRDLDF
                     RSDAVYGGRAISGGASCTGSGWSACGTGLGYWTGKMYRLTMGTCTSAPCSVMNWGVID
                     SGWLLPTEMVTAVPIAGSPVSLGPVTASSSVTLDSSGNTWIFFGTGRFMSNADKTDQH
                     AQYLVGVKDSVMSGCTQSVVSSCHDENLLDVTNAAICVSCASGNQVQGVGSVSTFTGL
                     TDKIQGNSAAGIPAMDGWVIQLASNSGSTTLGAERSIVSPTLIGGAVFFPTFTPSSDV
                     CVAAGTSQIYGLYYLTGTGYPDPIFGVDASGQAVRAVNGGQGVASSVAIQIGSEPTGM
                     SGYFQSSNSAINKITPKPPAMLWSQFMAWINQRV"
     gene            3376..4374
                     /locus_tag="VLD60_10205"
     CDS             3376..4374
                     /locus_tag="VLD60_10205"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HSB45375.1"
                     /translation="MTVRTPASEETARDKRSRAIPPARLPWKLLVVLALLCGCSPGEG
                     VGSDRLSRDKSNHPPVVKAVAILPTPLVLSDPLTVRVEAQDIDLNTLSFRYRWLVNGQ
                     VIAGQTRESLQPDLLKRGDQVAVEVTPFDGIIEGTPFLSGPASVVNTAPMLSHVVVDF
                     DHQAQGRQLLAKVDVVDPDHDPVSLTYRWRTNETVLKEGEENTLDLAGLTAQDTIQLD
                     VTASDGSPDGTATVTERFTLSNSAPTIISKPTLKPNGGQFDYLVQATDADGDPITYAL
                     EDGPPGMTIEANTGRIHWGVTPDLKGSYRVRVVAKDPQGGFTTQEFELSLKNTGQS"
     gene            4524..5588
                     /locus_tag="VLD60_10210"
     CDS             4524..5588
                     /locus_tag="VLD60_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSB45376.1"
                     /translation="MRKLKLREGTNTAVLFGHHDRHLKLIEEDLGVRLSARGEELTLD
                     GNPDATRYAERVLTELAGLANDGMLLQPDDITHALSALRQSPETPLKELLSNATTIVT
                     KKRFVAPKTPTQKSYIEAIETHDIVIGIGPAGTGKTYLAMAMAVSALMKREVSRIILA
                     RPAVEAGEKLGFLPGDMIAKVNPYLRPLYDALFDMMDMERATRAIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFNSKAVVTGDITQVDLPPERVSGLIEV
                     REILRDIEGIQFVYFDERDVVRHKLVQDIIKAYDQHQQAGQSSGQSTNPPGRRVSSTP
                     HHRPSQFPTSGSKDSSGQPH"
     gene            5589..6068
                     /gene="ybeY"
                     /locus_tag="VLD60_10215"
     CDS             5589..6068
                     /gene="ybeY"
                     /locus_tag="VLD60_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSB45377.1"
                     /translation="MPVLFQTHVRAVQFHETRLRRLAQTVLRDVGESSAELGLSFVGE
                     RRMRRLNRRFRHKDRTTDVLAFATREARLPHSSRLQAGLLGDVVISIPTAMRQARDGQ
                     RSLDQEIVVLLVHGILHLCGYDHERSEAEARRMQRREQTVLTRLGRIPRIARPARGR"
     gene            6122..7051
                     /gene="ftsY"
                     /locus_tag="VLD60_10220"
     CDS             6122..7051
                     /gene="ftsY"
                     /locus_tag="VLD60_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSB45378.1"
                     /translation="MGLFQRLSDGLTKTRSIVRGSLDRLLGRSADPALLEEFEAALIS
                     SDLGVHVTERVMKRLKESLEGTDASQAVRVQGALQQTLLDVLTPAAGPSLEQLIANGS
                     KPFVILVVGVNGVGKTTTIAKIAQRLVQGGRVPLLVAADTFRAAAIDQLQVWADRIGV
                     GLIRHRHGADPAAVAYDGLAAAKARHVDVVLIDTAGRLHTKSNLMDELRKIKRVIGQE
                     LPGAPHEVLLVLDATLGQNALAQARQFHQAVGVTGLALTKLDGTARGGIVVAIADELK
                     LPMRLIGIGEAVDDLQDFQARAFVDALLGLPAE"
     gene            7167..7952
                     /locus_tag="VLD60_10225"
     CDS             7167..7952
                     /locus_tag="VLD60_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45379.1"
                     /translation="MVKILVIDDDRMNCDLLQAVFTRHGYDVHTATSGLEGINLFRQH
                     APRVTILDLRMPEMDGLTVLKEIRAIDPHAPVIILGGGASEIQENQARALRVTDFVRK
                     GLSLDVLVEGVNRVAQQPVRRQDALGDTAVAGPVADTGETVLVVDDEQLVRDLLVQFL
                     SLRGYRTLGVKDGPQALSMVGQNAPDLILLDLMLPGMSGVEVLRQLRDRNFAGAIIIV
                     TGSYDEELLQEAWSLHPQEVIGKPIDLEKLLAIIQLVLVCREC"
     gene            7963..>9886
                     /locus_tag="VLD60_10230"
     CDS             7963..>9886
                     /locus_tag="VLD60_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HSB45380.1"
                     /translation="MVPCRRGLALASGLLLSFFSAAVPPLTAALDASPVGATIRHHDL
                     FVQIDPDGHRLVSTDRMTIDLSQPQQAVIFSLAPTLQLDRLVLSVGDKPGDEAANEIP
                     FEIEHDAAQARAQRIRIPAAILTAGKNTLTAHYHGLIHDPPREPRHLRFVTPSETAGH
                     IGPEGVYLSSESQWYLDIPDSLSTHRLRVALPPEWTAVTQGKPRTSAPCPSELCSQGN
                     FIMTEWDLVQPTEALTVVANRFVFKAREWVAKSGQSVQIAAYLFPENAPLAEEYLDAT
                     ARYVDAYIPLLGPYPFDSFAVVENFFASGLGMPSFTLLGSGIIKRHYVQPYALGHEIV
                     HSWIGNAVFNRVDSGNWVEGLTTYLANYYWHELSGEIQQARDQRRLMLRGYNLHVPPA
                     RDYPVGQFTQKHDERDNAIGYQKAAMVFHLLRQEVGDEAFWLGLKRLIAQYRGRHAEW
                     HDLEHIFSEAAGRDLRWFFIQWVERDGAPVLSIEGASAHPTGSEASRTFQLTVTVGQT
                     GHPFRISVPLIVRMDNGREQTIQGQASRAHDVITATLSDRPKEIVLDPDAMLMRRIPR
                     QALPPVLNHYVTDVKRSVVLAFSDPADGSHPFRGIVTRIEAQDSQRAVGERASTALLS
                     KDALLPREGSVLVLGTP"
BASE COUNT         2028 a         2825 c         2958 g         2075 t
ORIGIN      
        1 cggtcacgaa tattagaaac agtcaaaccc aggcctatgc acccttggcg gagtcgctgt
       61 acgaagcctt gtgtctttac cgcaaatcgc aaggcccctg ttacgacaac ggcaccggca
      121 cagtgtgggg cacgaattat actaacagca ccggcacgac gggcgatccc ttcttcttta
      181 ccagttataa ccagatggtg cggtgctgta agaattatgt cttgatgatc agccccggca
      241 tcggcgtggc cgacggaact aatccgacgc tccaaacgcc gtttccgacc ggctccacga
      301 tcaacccgac atggacgaac aatatcgggg tcaaagcatc cacgtcggcg ggcgatgcca
      361 cgtcggcagc tgctggtgat cgcctggacg acgtggccta ttacggacgc acgcacgatc
      421 tccgctccga cttgactggg agccaatatg tgaccttcta ttcggtgaat gcgatgggtc
      481 gcaaggtcgg cgcgaccttg ctcgcctccg ccgcaaagta cggcggtttt aatgatcaaa
      541 acaacgacgg tacgtcgaac ttcaatccga caagcggtca gacttgcaac tacccggcaa
      601 cctcgaatct caatccagga actgctggtc cgttctacag cgatccggaa tgggatgccc
      661 atggtcctct gcccactgac ccgccggatt gtgttccgga caccttcttc gatgccaatg
      721 acggctacgg tctcagaaac cagattctgg cggcgttgga cgccattctg aaaaactcgg
      781 catcaggcac ctcgatttca gtgctggcgt cctcctcgaa cggggacgga tcaatatacc
      841 aagcctattt ctatcctgag actcccaagg gcgacgggac cgccaatgtg gtgtcatgga
      901 caggctattt ccaaggcctt ttcatcgata cttttgggaa tctccgcgag gaccacggtg
      961 gggccagcgg cgccggggat ggcaaactcg tctataccga cgataacatc gttgtaaccg
     1021 aggtcagtcc aacgacgggc gaggtggtta tcagacgcta tgcggatgcc gacggagacg
     1081 gcaaggctga tgacacaggc tattatgcca ctccgcgaac agggggaacg gtcgcaacct
     1141 tgcgcgagat gcaagggatg tgggaagcgg ggaagaaact ggcgctgcgc gatctgagcg
     1201 ccaaaccgag aaacctgttt acctgggttg acctgaatga cgacggggtg gtgacggcca
     1261 acgagcaaat ggcgttcagc acggcgaatg cggcgacctt gtcgccgtac cttcgcgcaa
     1321 ccagcaccgg gacctttacc gcgacgaata tcatcagttt tcttcaaggc aaccaggtca
     1381 cagggatgcg agaccggcag tttcccgtcg atggtgttca aaaaacctgg cggttaggcg
     1441 acatcgtcaa ttcaagtccg accatcgtgg ggccgccgcg ggaacggttc gacattctct
     1501 atggcgacaa cgggtatcgt caatatctcc aacggtggtc caatcgacgc cagaccgcgt
     1561 atgtgggggc caatgacggg atgttgcacg ccttcaacct cggttattac catcgtgcgg
     1621 acgatcccaa cacctctgcc ctggagcacg gttggtatac gacggctcca tcggataaca
     1681 gcagcggtcc agagcttggg gaagaactct gggggtttgt gcctcattac atcctgccgc
     1741 agctcatgtg gtacacgcag tcgaattata ctcacatctc ctacgtggat ctgaaaccaa
     1801 aagtcactga cgtgcggatc tttaccccgg aggcggcctg tgggagcagc gcgagtccga
     1861 cacccacagc gactggctgt atccatccgg acgggtgggg cacgatcctc attatgggga
     1921 tgcggttcgg ggggagttgc ggtatctgtg cttcggtatc gggcgccaac aatggaggac
     1981 cgcccttggc cgtaacggca gatttcggga gcggagtcac gacgcgttat ttttatagcg
     2041 gttatatcgt tctcgacatt accgatccag acgccacacc aaaggtgctc agtgccttct
     2101 ccaactgggg cctgggcttg accacgagtt atccgacggt cgttcgcatg agcccgaaag
     2161 cggacgggaa aaacgaccat acaaacgccc gatggatgat gttgttgggg tctggggtcc
     2221 atggctacga tggacgggcc gccatgtatg gatcgatgtt cggcgtcctc cttgtgcagc
     2281 aggggacctc tcctagctat gttcagatgc cgatgctcga tcaggcgagc agtccgttga
     2341 cgtacaaaag cagttacatg agtgatctga tcaccttcga tcgcgatctc gatttccgct
     2401 cggatgcggt ctatgggggg cgcgccatca gcggcggcgc ttcgtgtacc gggagcggat
     2461 ggtccgcatg cggcacgggg cttgggtatt ggacgggcaa gatgtaccgg ttgacgatgg
     2521 gaacctgtac aagcgcccca tgctcggtga tgaattgggg agtgatcgac tcgggctggt
     2581 tgcttccgac ggagatggtg acggcagttc ccatcgcggg gtcaccggtc tcgctggggc
     2641 cggtcacggc cagctcctcg gtcacgctgg atagctcggg caacacctgg atctttttcg
     2701 gaaccgggcg gtttatgagc aacgccgata agacggatca acacgcccag tatctcgtcg
     2761 gtgtcaaaga ttccgtcatg agcggctgta cccagtccgt tgtctcaagt tgtcatgacg
     2821 aaaatctcct ggatgtcacc aacgcggcga tctgcgtctc ctgtgcgtcg ggcaaccagg
     2881 tgcaaggggt cgggagcgtt tcgaccttca caggcctgac cgacaagatc caaggcaatt
     2941 cggcagccgg tattccggca atggatggat gggtgatcca actcgcgtca aattcgggtt
     3001 cgacgaccct gggggccgag cgcagcatcg tgagtccgac cttgatcggc ggcgcggtct
     3061 ttttcccgac ctttacgcca agcagcgatg tttgcgtggc ggccgggacg agtcagatct
     3121 atggtctcta ttatctgacc gggacaggtt accccgatcc gattttcgga gtcgatgcca
     3181 gtggacaagc ggtgcgggcg gtcaacggcg gtcaaggcgt ggcctcctcc gtggccatcc
     3241 agatcggctc ggaaccgaca gggatgtcgg gttacttcca gtccagcaac tccgccatca
     3301 ataaaattac gccgaaaccg ccggcgatgc tctggagcca atttatggcc tggatcaatc
     3361 aacgggtcta attatatgac ggtgcgcacg ccagcctcgg aggagacggc gcgagacaaa
     3421 cggtctcgcg ccatcccgcc ggcccgtttg ccgtggaagc ttctcgtcgt actcgccttg
     3481 ctctgcggct gttcccccgg tgaaggagtg ggcagcgatc gactttcccg cgataagagc
     3541 aaccatcctc ccgtggtcaa agcggttgct attttgccca cgcccctggt gttgtccgat
     3601 cccctgacag tgcgggtgga ggcgcaggat atcgatctca acacccttag cttccggtat
     3661 cgttggctgg tcaacggcca agtcatagcc ggacaaaccc gagaatcgct tcagccggat
     3721 ctgttgaagc ggggagatca ggtggcagtc gaagttacgc ccttcgacgg aataatcgaa
     3781 gggacgccgt ttctatcagg tcctgcgtct gtcgtgaata ctgccccgat gctctcccat
     3841 gttgttgttg atttcgatca ccaagcccag ggccggcagc tgttggccaa agtcgatgtc
     3901 gtcgatccgg accacgatcc ggtttcactc acctatcgat ggcggacaaa cgagacggtc
     3961 ttgaaggaag gggaggagaa tacgctcgac cttgccggct tgactgctca ggataccatc
     4021 cagctggatg tgacggcgtc ggacggtagt cccgatggaa ccgccactgt tacggagcga
     4081 tttacgttgt ccaattccgc tccgacgatc atatcgaagc ctactcttaa gccaaacggt
     4141 ggtcagtttg actacctcgt ccaagcgacc gatgcggatg gcgatcctat tacctatgcg
     4201 ctggaagacg gtcctcccgg catgaccatt gaggcgaata ccggtcggat tcactggggg
     4261 gttacgccag acctaaaagg cagctaccgg gttcgtgttg ttgccaagga tccacaggga
     4321 ggttttacca cgcaggaatt tgaactttcc ttgaaaaaca cgggtcagtc atagcgggat
     4381 caagtcagtt acccaacgcg tctccattca agagcatgtc ccaccaactt gcgagctcgc
     4441 tttcagttgt cttcttcatt acccgtctga taagatttgc atagtgggaa tcgtcaagag
     4501 aggaggcgac cccgaacgtc cgcgtgcgta agctcaaact acgagaaggc accaacactg
     4561 cggttttgtt tggccaccac gatcggcatc tcaagctgat cgaagaagac cttggcgtgc
     4621 gcctctcggc acgcggcgaa gaactcaccc ttgacggcaa tcccgacgcc acccgctatg
     4681 cagaacgtgt cctgacggaa ctcgccggtc tggccaacga cgggatgctc ttgcagcccg
     4741 acgacattac tcacgcgttg tcggcactgc ggcagagccc ggaaaccccg ctgaaagaat
     4801 tgctctccaa tgcgacgacg attgtcacga agaagcggtt tgtcgcgccg aaaacgccga
     4861 cccagaaatc atatatcgaa gcgatcgaga cgcatgacat tgtgatcggc atcggtccgg
     4921 ccggaaccgg gaagacctac ctggcgatgg cgatggcggt gagcgccttg atgaagcgag
     4981 aggtcagccg catcatcctc gccagaccgg ctgtcgaagc cggcgagaag ctcggatttt
     5041 tgcccggcga catgatcgcc aaggtcaatc cgtatctgcg tcccctctac gacgcgctct
     5101 ttgacatgat ggatatggaa cgcgcgacgc gcgccattga gcggggcgac attgagattg
     5161 cgccgctagc gttcatgcgg ggccgcacgt tgaacgattc gttcgtgatt ctcgacgaag
     5221 cccagaacgc gacggctgaa cagatgaaga tgtttctcac gcggctcggg ttcaattcca
     5281 aggcagtggt caccggcgac atcacacaag tagacctgcc gcctgaacgg gtttccgggt
     5341 tgattgaagt gcgcgagatt ctccgggata ttgagggcat ccaatttgta tacttcgatg
     5401 agcgcgatgt cgtgcggcat aagctggtgc aggacatcat caaggcctat gatcagcatc
     5461 agcaggccgg acaatcgtcc ggtcaatcga ccaatccgcc agggcgacgg gtatcgtcca
     5521 cgccccacca ccgtccatca cagttcccca catctggctc caaagattcc tcgggccagc
     5581 ctcactaaat gccggttctg ttccagaccc atgttcgagc cgtgcaattc cacgaaacgc
     5641 ggctccggcg attggcgcag accgtgctcc gcgatgttgg ggaatcttcg gcggaattgg
     5701 gtctgtcgtt tgtcggtgaa cgccgcatgc gccgcctcaa tcggcggttt cggcacaagg
     5761 atcgcacgac ggacgttctg gcgttcgcga cgcgggaagc ccgtttgcct catagctcgc
     5821 gcctgcaggc cgggctgctg ggcgatgtcg tgatctccat tccgacggcc atgcgccagg
     5881 ccagggacgg ccagcgatca ctggatcaag aaatcgtggt gttgctggtc catgggattc
     5941 tgcatctctg tggatatgac catgagcgaa gcgaggccga ggctcgtcgg atgcagcggc
     6001 gggaacagac ggttcttact cggctcggca ggatccctcg catcgcacga ccagcgagag
     6061 gacggtgact tgtcggtcgg cattccatcg ggatagataa taggaaagag taggttgttg
     6121 catgggattg tttcaacggc ttagcgacgg actcaccaag acgcgctcca tcgtccgcgg
     6181 ttcgctggat cgcttactcg gccggtcagc ggatcccgct ttgctggagg aattcgaagc
     6241 ggcgctcatt tcgtccgatc tcggcgttca tgtgaccgaa cgggtcatga agcggctcaa
     6301 ggaatcgctc gaaggaaccg atgcctcgca agccgttcgg gtgcagggag cgttacagca
     6361 gacgctgctc gatgtcttga ctccggcggc cggtccttct ttggaacagc tgatcgcgaa
     6421 cgggtcgaag ccgtttgtca ttctggtggt cggcgtgaac ggggtgggga aaaccaccac
     6481 gattgcgaaa atcgcccagc ggcttgtgca gggaggccgt gtcccgctcc ttgtcgccgc
     6541 cgacaccttc cgagctgcgg cgattgatca gctgcaggtc tgggcggatc gcatcggggt
     6601 tggactcatc cgccatcgcc acggagcgga tccagcggcc gtcgcctacg atggattagc
     6661 cgctgcgaag gctcgacatg tcgatgtcgt cttgatcgac acggccggcc ggcttcacac
     6721 caagtcgaat ctcatggatg agcttcgaaa gatcaaacgg gtgatcgggc aggaacttcc
     6781 cggcgctccc catgaagtct tgctggtcct ggacgcgacg ctcggtcaga atgccctcgc
     6841 gcaagcgcgt caattccatc aggccgtcgg ggtgacgggc ctggcactga ccaaattgga
     6901 tggcaccgca cggggcggaa tcgtcgtggc catcgctgat gaattgaaat tgcccatgcg
     6961 gctgatcggc atcggcgagg cggtcgacga tctccaggac tttcaggcga gggccttcgt
     7021 cgatgccctg cttggcctgc ctgccgagta gagagacgtc gctccgcgca ttcgcctctt
     7081 ttccttctca agattctcct cccatgatag gctccgtcca tgtgtcaaga tggggcggtt
     7141 ctgtgcagat cctgtgaggt tcgctgatgg taaagattct ggtcatcgat gacgaccgca
     7201 tgaattgcga cctgttgcag gcggtcttca cccgtcatgg gtacgacgtc catacggcga
     7261 ccagcggcct tgaagggatc aacctctttc ggcagcatgc gccccgtgtg acgatcttgg
     7321 atctccggat gcccgagatg gacggcctaa cggtgctgaa ggaaatccgt gccatcgacc
     7381 cgcatgcgcc cgtcattatt ttgggtggcg gcgcatcaga aattcaggag aatcaagctc
     7441 gcgctttgcg cgtgacggat tttgtgcgca aagggttgtc gctcgatgta ttggtcgagg
     7501 gtgtgaaccg agtagcccag cagccggtca gaagacagga tgcgctcggt gacacagccg
     7561 tagccggccc ggttgctgac accggtgaaa cggtgctcgt ggttgacgac gaacagctgg
     7621 tgcgcgatct acttgtgcaa ttcctcagtc tgcgtggcta tcgcacgctc ggcgtcaaag
     7681 atggtcccca agcgctctcg atggtgggac agaacgcccc ggacctcatc ctgctggatc
     7741 ttatgctgcc gggaatgagc ggtgtcgagg tgctccgcca gctacgtgac aggaacttcg
     7801 ccggcgcgat cattattgtc accggcagtt atgacgaaga actgctgcaa gaggcctggt
     7861 ctctccatcc ccaagaggtc atcgggaagc ccatcgatct tgaaaaactg ctcgcgatca
     7921 tccagcttgt gctcgtctgc cgcgaatgct aaaagtcttc aaatggtacc ttgcagaagg
     7981 gggctggctc tcgcgtcagg actcctgctc tcgttcttca gtgcggcggt tcctccctta
     8041 actgcggcgc tagatgcctc gccggtaggc gcgaccatcc gacaccatga tctgttcgtg
     8101 cagatcgacc ccgacggcca tcggctcgtt tcaacggatc ggatgactat cgatctgtcg
     8161 cagccacagc aggccgtcat tttttcactc gctcccaccc tgcaactcga ccggttggtt
     8221 ctatccgtcg gggacaagcc tggggatgag gcagccaacg aaattccatt tgaaatcgaa
     8281 catgatgccg cacaggctcg agcgcagcgg atcaggatcc cggcagcgat cctgacggct
     8341 gggaagaaca cactgacagc ccattaccat ggtctgattc atgatccgcc gagagaaccc
     8401 cggcatctgc gattcgtcac cccgagtgag acggccggtc atatcggtcc cgagggggtc
     8461 tatttgagca gtgaaagcca atggtatttg gatatccctg actcgctcag cacccatcgg
     8521 ctgcgcgtag ccctgccgcc tgaatggacc gcggtcacgc aaggaaagcc tcgaacctcg
     8581 gctccgtgcc cttccgaact ctgctctcag gggaatttca tcatgacgga atgggatctg
     8641 gttcagccga ccgaggcgct gaccgtcgtt gccaatcggt ttgtcttcaa ggcccgtgag
     8701 tgggtggcga agagcggaca atccgtacag attgccgcct atctgtttcc cgagaacgct
     8761 ccgttggcgg aggagtatct cgacgccacg gcccggtatg tcgacgccta tattccgctg
     8821 ctcggtccct atccattcga cagttttgcg gtcgtcgaaa actttttcgc cagcgggttg
     8881 gggatgccct ccttcaccct gttgggaagc gggattatca agcggcacta cgtgcagccc
     8941 tatgccttgg gccatgaaat cgtccattcg tggatcggca atgccgtgtt caatcgggtc
     9001 gacagcggaa attgggttga aggtctgacg acgtatctgg cgaattatta ctggcatgag
     9061 ttgtccggcg agattcaaca ggcgcgcgac caacgtcgcc tgatgttgcg cgggtacaat
     9121 ctgcacgtac ctccggcccg cgattatccg gtcggacaat tcacgcaaaa gcatgacgag
     9181 cgtgacaacg cgatcggtta tcaaaaagcc gccatggtct ttcatctcct gcgacaagaa
     9241 gtcggagatg aggcgttctg gctggggctc aaacgtctta tcgcacagta tcgtgggcgg
     9301 catgcggaat ggcacgatct ggagcacatc ttctcggagg ccgccgggcg cgatctgcgt
     9361 tggtttttta ttcagtgggt cgagcgggat ggggcgccgg tcttgtcgat cgaaggcgca
     9421 tcggcgcatc cgacaggcag cgaggccagc cggacgtttc agctcacggt cacggtcggc
     9481 caaaccggcc atccgtttcg gatatccgtg ccgctgattg tccgtatgga caacggccgt
     9541 gaacagacca ttcaaggcca agcgagcagg gctcatgacg tcatcacggc cacattgtcg
     9601 gacaggccga aagagatcgt gcttgatccg gacgcgatgc tgatgcggcg aatcccacgg
     9661 caggccttgc ctccggtgtt gaatcactat gtcaccgatg tgaagcggtc cgttgtgctc
     9721 gcgttttctg atcccgcgga tggatcccat ccctttcgcg gaattgtgac gcgcatcgaa
     9781 gcgcaagaca gccaacgggc ggtaggggaa cgggcatcaa cggcgcttct ttccaaagac
     9841 gctctattgc cgcgagaagg ttcggtgcta gtattgggga ccccgg
//
LOCUS       DATIFR010000178        17460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_113608, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000178 DATIFR010000000
VERSION     DATIFR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113608"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..529
                     /locus_tag="VLD60_10235"
     CDS             101..529
                     /locus_tag="VLD60_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB45381.1"
                     /translation="MAVQVYGCNHVVIEVTDAKKAVKFYSDVFGLKMLRGGEGAAWCK
                     LGEHQFMAIFEVVKLQPDRVKHFGLMVRDAQQIKEVRHKLVKKYKLKLHPGFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQETGITFAKSGGRRG"
     gene            533..916
                     /locus_tag="VLD60_10240"
     CDS             533..916
                     /locus_tag="VLD60_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45382.1"
                     /translation="MDDTQVQLDQLSRALLRLHKALLDGERVAYEQVHGPVTSNGAFL
                     QLLLGHAWFAWLRELSSAMAALDELAESKEASARAAIIEQMAALRILLTPSETGEGFS
                     HRYRDALQRDPNVVQAHAVVTELLR"
     gene            942..1388
                     /locus_tag="VLD60_10245"
     CDS             942..1388
                     /locus_tag="VLD60_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB45383.1"
                     /translation="MKAHYLGHVVFYVKDLERSLGFYRDLLGFKEVGRIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPAGRRRGLYHIGIKVGDSLDELRAAKKELEQAGVPIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDASESIWKNNPQAVLSPIKPLRL"
     gene            1411..2283
                     /locus_tag="VLD60_10250"
     CDS             1411..2283
                     /locus_tag="VLD60_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB45384.1"
                     /translation="MGLSKWLPFMLVVGYLFVQGLGVSAQQLRPDDEVTKLADDVYLF
                     RHKFHQSIFITTPKGVVVTDPISSDAATWLKAKIKTLTDQPVRYVIYSHHHNDHITGG
                     SVFADTALFISQTAARPKILQAADPQTPVPDLTFSDRMFIDLGGKRFELIYTGRNHSD
                     NSLVVLLPQDRLLFAVDFIPVETVAYRALPDGYPDEWIDSLKQVEGLEFDTLVPGHGK
                     IGKKEHVQQFRVYLEDLRAAVLEQVQKGVGIEEAKKNVQLPKYDQWHRYAEWFPENVE
                     GMYRYLSEQRKENR"
     gene            2339..2980
                     /locus_tag="VLD60_10255"
     CDS             2339..2980
                     /locus_tag="VLD60_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB45385.1"
                     /translation="MKIVVIGATGFVGSAILHEALQRGHEVTAIVRHPEKLTAHPKLY
                     PQRGDVYSADDVARLVAGHDAVISAFNPGWSNPDIYKLQVNGTQAIINGVKKAGIKRL
                     LFVGGAGSLEVKPGVQALDLPGFPAEYKQGALATREALNLLRKESTLHWSFLSPSADL
                     SPGQRTGKFRLGTDQLLKDANGESRISVEDYAMAMIDELENPKHIRRRFTVGY"
     gene            3059..3286
                     /locus_tag="VLD60_10260"
     CDS             3059..3286
                     /locus_tag="VLD60_10260"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSB45386.1"
                     /translation="MSDVIITPKNNGPYYIKGTFKIVTEGGREIASDGNEAWLCRCGQ
                     SANKPFCDGSHKKAGFKNNLDERSLSPGQVA"
     gene            3455..3880
                     /locus_tag="VLD60_10265"
     CDS             3455..3880
                     /locus_tag="VLD60_10265"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB45387.1"
                     /translation="MKRTASAVWQGDLKSGKGTVSTESGVLSKTQYSFGTRFENGKGT
                     NPEELIGAAHAGCFTMALSAQLGNAGLVADRLESTATLSFEKLEAGWTVTEIHLDVRG
                     RVPKADQAAFERAAAAAKTGCPISRLLNTTITMDATLET"
     gene            3988..6039
                     /locus_tag="VLD60_10270"
     CDS             3988..6039
                     /locus_tag="VLD60_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB45388.1"
                     /translation="MDRYSASPCETLAERYQALLEVAQAISAHRDIHELFRDLAQRLP
                     RVVHVNFVALSLHDPERNTMRLHTIQANVPADLIGGHEEQVEETPAGLAWRTQQPVLV
                     PDLADERRWPNVTQRMKEDGTNSFCVVPLTTAVRRLGAMGFSSLQKEAYAETDLEFLQ
                     QVGKQVAVAVDNVLHHQDLTHDRDRLRLLLEVSESIASHRDLEELFSDLAQRLPQIVP
                     FDYINAVLHEPARDVMRLWLLVTATPSTISPGLELPVDESPGGLVWKTQQPLTVNDVT
                     QERRFTRLMAMLRENGVQSFCVVPLTTAQRRLGAMGFGSLQQRAYQEAEINFMQQVAK
                     QIAVAVENALGYKQIAELKEKLSKEKLYLEEEIRTEYNFEEIIGESTTLKKVLKQVEV
                     VAPTDSTVLILGETGTGKELLARAIHNVSERRERTFVKINCAAIPTGLLESELFGHER
                     GAFTGAIATKVGRFELADGGTLFLDEVGDIPLELQSKLLRVLQEQEFERLGSTRTLKV
                     NVRVVAATNQDLSDMVEKKEFRNDLYYRLNVFPLMIPPLRERREDIPVLVRYFTQKFA
                     RQLNRPIERIPADTMDALTAYAWPGNIRELENLVERAVILSTGLDLVVPLSELKASRA
                     ATHTAQVSTLESAEREHIIRALNATNWIIGGPAGAAVKLGMKRTTLQSKMQKLGVTRP
                     S"
     gene            6850..7329
                     /locus_tag="VLD60_10275"
     CDS             6850..7329
                     /locus_tag="VLD60_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB45389.1"
                     /translation="MRITKLALCLIFAGTPALSIAAGHDVNAQTSGPQVTTIISKTDV
                     AGIKDKEWKVRTIELAPGAVDARLFYSGVELVYVLEGAGRLEVDGKPFVALHPGAVAA
                     LNPKEIHVLKNTNQTQTLKVVVVLLREKGQPPLAFGNRGTPSKAVEQDNSTVPRLVF"
     gene            7504..8571
                     /locus_tag="VLD60_10280"
     CDS             7504..8571
                     /locus_tag="VLD60_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HSB45390.1"
                     /translation="MTLFIQNSAFSIQSSGLLDSLTLLIKTVQLRPYVFLFLAAFLLS
                     APALIGWQRTGLFFAVVWLTAFVCEFSSTRTGLPFGWYHYTGSTVGQELYLSNVPFMD
                     SLSFTMLLYASYCLALSFLLPSHSERGRKSPFSKNGRLLALTLDRSVRTGWPVCMLTT
                     LFFVFIDMVIDPVALRGDRWFLGKIYYYPNPGLHFGVPLANYGGWAVVGVSALLIYFV
                     LDRRLPDLELRSHESVTSNILMGCSLYYGVLLFNLTVTFWIDEPLIGMTGILIYIPLT
                     VLLLLRLLGKLPAPATLSPPDHRMVDGSRLVTVLHRSHKHGPSSWREAGSEWLESSAH
                     HRGDRLLGQDARTLDTIGRSG"
     gene            8571..9620
                     /gene="hpnH"
                     /locus_tag="VLD60_10285"
     CDS             8571..9620
                     /gene="hpnH"
                     /locus_tag="VLD60_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HSB45391.1"
                     /translation="MAVPLSQMVTVAQYVLTQQLKGVQRYPLVLMLEPLFRCNLACAG
                     CGKIQYPNHVLDTRLTPEQCWAAVDECGAPMVSIPGGEPLIHPEMPEIVGGIVARKKY
                     VYLCTNAILLERKLDQYEPSKYLTFSIHLDGLREDHDHAVCRDGVYDVAVRAIKAALE
                     RGHRVTTNTTLFSDANPLRVRRFFDEMMVLGVEGMMISPGYSYTKAPDQEHFLRRQKT
                     HMLFADILSNRKRSWRFNQSPLFLEFLMGKLDYQCTPWGNPTYNIFGWQKPCYLLQDG
                     YVRTFRELLDGTDWDRYGTARNEKCADCMVHCGYEASAVTDTFGSWGGFGRTVWAMLA
                     PNTRWRKRPPSILHV"
     gene            10064..11020
                     /locus_tag="VLD60_10290"
     CDS             10064..11020
                     /locus_tag="VLD60_10290"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSB45392.1"
                     /translation="MNEALHFLTQHGTLVLFAAVFAEQIGLPLPAVPFLIAAGALAGT
                     GQMTLGMAVGSAVLAALAADQVWFELGRRRGRRVLNWLCRISLEPTSCVRRTEEFFAR
                     HGVRSLVVAKFIPGLSTIAPPLAGIVGLRVPQYLLFNGLGTILWVGSGIGLGYVFSDQ
                     LERTVSVTAHVGPTVGLVLLGGVIGYVIYKALHRYRMERLVPRMNAQQVSEKIAAGED
                     PLIIDLRPVGDRHDIPGIPGSLTRSLEELIARHHDLPRDRDVILYCACPKDAASVQAA
                     WKLREKGLTRVWPLAGGIEAWHAIALESGGAIRVTEGHTIAV"
     gene            11110..11847
                     /locus_tag="VLD60_10295"
     CDS             11110..11847
                     /locus_tag="VLD60_10295"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB45393.1"
                     /translation="MSSLTKKVAIVTGSSDGIGRAIAERLAQDGATVVVNYGKSADKA
                     KQVVAGIESKGGKAVAVQADMSKVGDVGRLIKDTVKTFGGLDILVNNAGMFMFKPLAE
                     TTEEEFDRMFALNTKGPYFALQEAAKVIKEGGRIVNISTDGTHIGFAGATAYLGSKGA
                     LEQFTKGLAHELAPKGVTVNTVSPGYTITAMLPADPAFRQIGEQASPLKRLGTPKDIA
                     DVVAFVVSEEARWLTGQNIHAGGGVVM"
     gene            11865..12329
                     /locus_tag="VLD60_10300"
     CDS             11865..12329
                     /locus_tag="VLD60_10300"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HSB45394.1"
                     /translation="MKENLEEVKLLARDLRNGKEFPRSPRETLAGYVLAARAVDKCRA
                     VLVGWQGEYHSNCPLDQRWLKFAEIDYGAFRRFVATGATDDKIAAWIVEHAKKRSRAD
                     IVVWNNKERDLSLSDLTPELQEYMEDYIASCVPRHRVVYRWFDVYDLEEQRL"
     gene            12416..13459
                     /locus_tag="VLD60_10305"
     CDS             12416..13459
                     /locus_tag="VLD60_10305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HSB45395.1"
                     /translation="MLPTRGYATKSPTSILEPFNFERRDPGPRDVLIEILYCGVCHSD
                     IHQARDEWGGSIFPMVPGHEIVGRVARVGSQVTRFKVGELAGVGTVVDSCRVCESCKE
                     GLEQYCEKHPSWTYNGTEQDQKTPTYGGYSSHIVVDDHYTFKLSPNLPLANVAPLLCA
                     GITTYSPLRHFNVGPGQKVGIVGLGGLGHMAVKLAASMGAEVTVFSTSKTKEQDARRL
                     GAHDFVVSLDPKNFAALANRFHFILDAVSAPHDVNAYLNLLRRDGVLTMVGVPAKPLE
                     IQAFSLILKRRKLAGSGVGGIRQTQEMLDYCAQHNITADVEVIPIKQVNEAYDRTLKG
                     DVRYRFVIDMKTL"
     gene            13504..13785
                     /locus_tag="VLD60_10310"
     CDS             13504..13785
                     /locus_tag="VLD60_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB45396.1"
                     /translation="MREKGTTAMHFMTMAKATNDLQAGVMPEEKLTAEKATHQEEPGK
                     TGVQLDASGLQPSSEGSRMKDSGGRRMVIDGPFAETKEVMGGHMLTQAK"
     gene            13839..14624
                     /locus_tag="VLD60_10315"
     CDS             13839..14624
                     /locus_tag="VLD60_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45397.1"
                     /translation="MPLGVTWTIVSSLVLIACSVWALITFYARGSSLDTAARRRFGIA
                     SGMYLFGWLVLAFVLGSNGIFQATPSRAFPALALGIALLILTGAWLLYRSSALKTVLA
                     TVPLPWLVGVQLYRVLGLNFLVLYSLGRLPGEFAIPAGWGDIAVGLAAPVVGYLLYKG
                     YRWSCLAALRWNVVGILDFVVAVTTGFLSSPGPFQALALENPNMLITAFPLVLVPLYA
                     VPLSILLHLAVLKRLKVTLGAPSDKELECGSQSGHWGLHPVAH"
     gene            14677..15423
                     /locus_tag="VLD60_10320"
     CDS             14677..15423
                     /locus_tag="VLD60_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB45398.1"
                     /translation="MTDNARVTEIAPDHFAISIYVKEFNLRFNHFLIKDEEPLLFHTG
                     MKQMFPFVRDAVARVIDPSMLRWISFSHFEADECGALNDWLALAPHAEPICGLVGALV
                     SVNDFSIRQAHVLAHDQILKTGKYRFRYRQTPHVPHNWEAGLLFEEVTRTLLCSDLFS
                     HEGDVEPVTESDVVERARHALIEGQKGSFANAYPYTPLTEPILHGLADLRPKQLALMH
                     GSSFVGDGERALRDLATVMREVLGASTARR"
     gene            15445..15816
                     /locus_tag="VLD60_10325"
     CDS             15445..15816
                     /locus_tag="VLD60_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB45399.1"
                     /translation="MAIELNHTIVPAKDKVASAKFFAMMFGLSFDENAVGYFAPVKVN
                     ETLTLDFHDDVDRFEVHHYAFKVSEPEFDAIFTRVQEAGIPFGRGPGNLEDGKLNSSN
                     GGRGVYFRDPNGHILELLTVG"
     gene            16283..16651
                     /locus_tag="VLD60_10330"
     CDS             16283..16651
                     /locus_tag="VLD60_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45400.1"
                     /translation="MVVAGTALANPKDEGEQGMLKITKHRDAGSDSVSFKLEGRLAGP
                     WVGELDTCWRQMTASPQGRTVVDLTGVTFVDGEGKALLTRMWQQGVELRAAGCLTRCI
                     VEEIAKTGQADASRSRRKDK"
     gene            16905..>17460
                     /locus_tag="VLD60_10335"
     CDS             16905..>17460
                     /locus_tag="VLD60_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB45401.1"
                     /translation="MWGCKQEAASTPARPIPEVPVVAATVQNVPDEPEFIGQTESSRP
                     VEIRSQVTGIIKERFFAEGRAVKKGDRLYQIDPVPFKAALLSAKAKVAQAEARLVQAK
                     QNLARVKPLLAEQAVSQKDVDDAVAEELAAVAALDGAKADLVKAKFDFDNTFIIAPIN
                     GMIERTRVYEGRLVSAQTDLLTIVH"
BASE COUNT         3695 a         4807 c         5253 g         3705 t
ORIGIN      
        1 ctcgggtgcc cagccggtcg acgtgtttat gtcggcgatg cagaatgcac gagcccaagc
       61 ctctgtcgcg gtcaaaagct cgtcagtcgg gaggtgacga atggcggttc aagtctatgg
      121 ctgcaatcat gtggtaatcg aggtcacgga tgccaagaaa gccgtgaagt tctattcgga
      181 cgtttttggt ttgaagatgc tccgcggcgg cgaaggggca gcctggtgca aactcggcga
      241 gcatcaattc atggcgatct tcgaggtggt caagcttcag ccggatcgcg tgaaacattt
      301 cggcttgatg gtccgcgatg cgcagcagat caaagaagtt cggcacaagc tggtcaagaa
      361 atacaaactg aagctgcacc ccggtttccg ctgtgatttt cgtgatccct ggggtaaccg
      421 gattcaggtc ggcgatctga gcgatgagtc gctcgtctgg ctcctcccct atcaggaggt
      481 gcaggagact ggtatcacgt tcgcgaagtc aggaggccga cgaggctgac gcatggatga
      541 cacacaggta cagctggacc aactctcacg ggcgctgctt cgtctgcaca aggcgttgct
      601 ggacggcgaa cgggtagcgt acgagcaggt gcacggccct gtcacttcga acggagcgtt
      661 tctccagctc ttgctcggtc atgcctggtt cgcctggctg cgtgagctct cgagcgcgat
      721 ggctgcgctg gatgagcttg ccgaatcgaa agaggcttct gcccgtgctg ccatcatcga
      781 gcagatggcc gcgctgcgga ttctcctgac gccatctgaa acgggtgaag gattcagcca
      841 tcgttaccgt gatgcgctcc agcgcgaccc aaacgtcgtg caagcgcatg cggtggtgac
      901 ggagttgctc cggtaacttt cgtccggagt cggaggacaa catgaaagcg cactatctcg
      961 gccatgtcgt gttctatgtg aaggacctcg aacggtcgct cggcttctac cgcgacctct
     1021 tgggattcaa ggaagtcggg cgcatcttca acggagccgc ggcggcgctc acatccggtc
     1081 gcacgcatca cgagttattg ttgattcaag ttggtgacgc accgggtccc ccggccggaa
     1141 ggcggcgagg gctctatcac atcggcatca aagtcggcga tagcctcgat gaattgcggg
     1201 cggcgaagaa ggaattggag caggccggcg ttcccatcga cggcatgagc gatcacacgg
     1261 tgagccagag tctgtatctg cgcgatccgg acggcaacga agtagagctg tacgtggatg
     1321 cgagtgaatc catctggaag aacaaccccc aggccgtgtt gtcaccgatc aaacccctgc
     1381 ggctttgagt tattcgaagg aggcgctctg atgggtctct cgaagtggct cccattcatg
     1441 ttagttgtcg gatatctgtt cgttcagggt ttaggggtgt ctgctcaaca gctccggccc
     1501 gatgatgaag tcaccaaact ggccgatgac gtatacctct tcaggcataa atttcatcag
     1561 tccattttca tcaccactcc gaagggcgtc gtcgtcacag atccgatcag ttctgacgcg
     1621 gcgacctggc tgaaagcgaa gatcaagact ctcaccgatc agcccgtccg ctatgtgatc
     1681 tatagccacc accataatga tcacatcacc ggtggcagcg tcttcgccga cacagctctt
     1741 ttcatcagtc agactgccgc gaggccgaaa attctccaag cagcggatcc tcagacccct
     1801 gtccccgact tgacgtttag tgatcggatg ttcatcgatc ttggcggcaa acgcttcgaa
     1861 ctgatctata ccggcaggaa tcattcggat aacagtctgg tcgtcctgct tccgcaggat
     1921 cgactcctct tcgccgtgga cttcattccg gtcgagacgg tcgcctaccg ggcgctgccg
     1981 gacggctatc cagacgaatg gatcgactcg ctgaagcagg ttgaaggcct cgagttcgac
     2041 acgctcgtgc cgggacacgg caagatcggc aagaaagagc atgtgcaaca gtttcgcgtc
     2101 tatctggaag atctcagagc cgccgtgctg gagcaagtac agaagggtgt cggtatagaa
     2161 gaagcgaaga aaaacgttca gcttcccaaa tacgatcagt ggcaccgtta cgccgaatgg
     2221 ttcccggaga atgtggaagg gatgtaccgg tatctctctg agcagcggaa agaaaatcgc
     2281 tgagaaccgt ctcgggctgc tggtggggat gaaacaatga tccgacggga ggttgaccat
     2341 gaagatcgta gtgatcggcg cgacggggtt tgtcgggtcg gcgatcttgc atgaagcctt
     2401 gcagcgcggc catgaggtca ccgccatcgt gcggcatccg gagaaactta ctgcgcatcc
     2461 gaagctctat cctcagaggg gagatgtcta cagcgcagat gacgtggccc ggttggtcgc
     2521 gggccatgat gccgtcatca gcgcgttcaa tcctggttgg agcaatcctg atatctacaa
     2581 acttcaggtc aatggcacgc aggccattat caatggtgtg aagaaggccg gaatcaagcg
     2641 gctgttgttc gtgggaggcg caggcagctt ggaagtgaag cccggagtcc aggccttaga
     2701 cctgccgggc tttcccgccg agtataagca gggcgcgctt gcaacccgcg aggccctgaa
     2761 cctgctgcgg aaggaatcca ccctgcactg gtcatttctc tccccttcag ccgacctgtc
     2821 tcccggccag cgcaccggca aattccggct tggaacggat cagctgctga aagatgccaa
     2881 cggagaaagc cgaatctctg tcgaggatta tgcgatggcg atgatcgatg aattggagaa
     2941 tccgaagcat atccgtcggc ggtttacggt gggctattag caagggaacg cagcagactc
     3001 cttcgcagta gtaagatggt cgtgcgccgt gtgtacgtaa caactcaagg aggacaacat
     3061 gagcgacgtc atcattacac ccaagaataa cggtccgtat tacatcaagg gaacgttcaa
     3121 gatcgtgacc gagggcggtc gggaaatcgc ttccgatgga aacgaggcct ggctctgccg
     3181 atgcggtcag tcagcgaaca agcccttctg cgacggctcg cacaagaagg ccggtttcaa
     3241 gaataacttg gatgagcggt cgttatcgcc cggccaagtt gcctaggaag cgctgcatcg
     3301 cggttcgctt gtgaatggga ggaacatgcc ggctatgcca tagatcggca agtgctggct
     3361 cgtctcccgc caccggacgc gatcatcact cgcatcggag acttgccaac catgatctct
     3421 gatctctcga cacgacagat agggaggtga aaccatgaaa cgaacagcct cagcggtatg
     3481 gcagggcgat ctgaagagtg ggaaaggcac cgtctcaaca gaaagcggtg tactgtcgaa
     3541 gacccagtac tcattcggca cgcggttcga gaacgggaag ggcaccaacc cagaagagct
     3601 gatcggtgcg gcacatgcgg ggtgctttac gatggcgctc tcggcgcagt tggggaatgc
     3661 cggtctggtc gccgacaggc tggagtcgac ggcgacgttg agcttcgaga aactcgaggc
     3721 aggctggacc gtaacagaga tccacttgga cgtaaggggc agagtcccga aggccgatca
     3781 ggcggcgttc gagagagccg cagccgcggc caagacgggc tgcccgatct cacgtctgct
     3841 caatacgacc atcacgatgg atgcgacgct ggaaacgtga cctgtcagtc gataacgaga
     3901 cttcaacgtc catctgaaac cgcatctgcc acaggagcag cggagcctat tgagccggca
     3961 cgggctgata ggcgatacta agaagctatg gataggtact ccgcatctcc ctgtgagact
     4021 ctcgcggagc gataccaagc cctgctggag gttgcacagg cgatctccgc acaccgcgat
     4081 atacacgaac tctttcgcga tctcgcccag cgcctgcctc gagtggtgca tgtcaatttc
     4141 gtcgccctgt ccctacatga tcctgaacga aacaccatgc gattacacac gattcaggca
     4201 aatgtgccgg cggaccttat cggcggccac gaggagcagg ttgaagagac tccagccgga
     4261 ttggcatggc gaacgcagca gcccgtcctg gtgccggatc ttgccgatga gcgtcggtgg
     4321 ccgaacgtga ctcaacgtat gaaggaggac ggtacgaatt cgttttgcgt cgtgcctctg
     4381 accacggcgg ttcggcggtt gggcgcaatg ggattctcga gcttgcagaa agaagcgtat
     4441 gccgaaacgg acctcgaatt cctccagcag gtcggcaaac aagtcgccgt tgccgtggac
     4501 aacgtcctcc atcatcaaga cctgactcat gatcgtgacc ggttgcgtct cctcctggag
     4561 gtttctgaat cgattgcgtc acaccgcgac cttgaagagc tgtttagcga tctcgctcaa
     4621 cggcttcctc agatcgtccc gttcgattac atcaatgcgg tgttgcatga gcctgcgcgc
     4681 gatgtcatgc gcctctggct cttggtaacc gcgacaccca gtacgatcag ccctgggctg
     4741 gagctgcccg ttgatgaatc tcccggagga ttggtgtgga aaacccaaca gccgttgacc
     4801 gtgaacgacg tgacgcaaga gcgcagattt acgaggctga tggccatgtt gcgcgagaat
     4861 ggcgtccagt cgttctgcgt ggttcctctc accacggcgc agcgtcgcct aggcgccatg
     4921 ggatttggaa gcttgcaaca gagagcctac caggaagccg agatcaactt tatgcagcaa
     4981 gtggcgaagc agatcgcggt cgcggtcgaa aatgcgctgg ggtacaagca gattgccgag
     5041 ctcaaggaga agctctcgaa ggagaagctg tatttagaag aagaaatcag gaccgagtat
     5101 aacttcgagg agatcatcgg tgagagcacg acgctgaaga aagtgctgaa gcaggtggag
     5161 gtcgtcgcac cgaccgattc gaccgtgttg atcctgggag aaacagggac ggggaaggag
     5221 ttgctggccc gtgcgattca taacgtcagc gagcggaggg aacggacctt cgtgaagatc
     5281 aactgcgcgg ccattccgac cggcctgctc gaaagcgagt tgttcggtca cgagcgaggg
     5341 gccttcaccg gggcgatcgc caccaaagtc ggacggttcg agctggccga tggtggaacg
     5401 ctctttctcg acgaagtcgg agacattccg ctcgaactgc agtcgaagct cctgcgcgtc
     5461 ctgcaggaac aggaatttga gcggttggga agcacgagaa ccctcaaggt gaacgtacgg
     5521 gtggtggccg cgaccaacca ggatctttcc gacatggtgg agaaaaagga gtttcggaac
     5581 gatctctatt accgtttgaa tgtgtttccg ctcatgatcc caccgctgag ggagcgccgc
     5641 gaagatatcc ccgtcctcgt ccgctacttc acacagaagt tcgcgcggca gttgaatcgt
     5701 ccgatcgaac gcatcccggc tgacacgatg gatgctttga cggcgtatgc ctggcccggg
     5761 aacattcgtg aactcgaaaa tctggtcgaa cgggccgtga ttctctccac cgggctggat
     5821 ctggtggttc cgctcagcga actgaaggcc tcacgtgctg cgacgcatac agcgcaggtg
     5881 tccacactgg aatccgcgga gcgcgagcac attatccgcg cgctgaatgc gaccaactgg
     5941 atcatcggag gtccagccgg tgcggcagtc aagctcggga tgaagcgaac gaccctccaa
     6001 tctaagatgc agaagctcgg cgtcactcgg ccttcctagt ccggactacc cgcgaagctt
     6061 cgattgtttc gggcttgcgc cgatccttcg gcactgtgcc gctatgttgg cactgctcca
     6121 tcgtcacaac actcccacat cacacccaga gtcccctgct gctgaacgac aacccctttg
     6181 ctgtccgtga cttgtcttgt gagttttgat tcgtcagtcg cctggaacga tctatgccgt
     6241 gctcaagagt agacaacgga attgaagtga cgcaagtgat ggtgaattcg atatcacttt
     6301 aaaaggagct gacaccatgt cctggctggt tgtgttccta tcatcttttt tcccgatcgt
     6361 gctcctgttc gtcatacttg ccgtggattg gctgcagagg gaggccgggt cgagaccttc
     6421 ctcgctcagg cttcagaagt gtgaggaaaa aacaataaga gggagaggtt aactggcatg
     6481 caatgcctgc acgctgttca cgaaggcctt atgactgcta accagtcccc gtcgggtatg
     6541 caacagaagg agacaccatg aacaaaggag gcccgtcgat ggccatggca gtgggagtta
     6601 cagcgaaaga cttgaacgag gagaattcga taggttggaa gaaacttgcc agcgcgacga
     6661 ggtgttcgcg ctgcggaggg ctcatggtca tcgagcagcg cttcggtctc atgcgtcacg
     6721 gtggcgcccc tgattcgcaa gcctggcgct gcgtgcagtg tggggagttc atagatccgg
     6781 tcattctgca aaaccgtcaa cggtctcttc caatcggcgc tagttgaagc tgatctaggg
     6841 gaggaaacta tgagaatcac gaagttggct ctatgtttga tattcgcagg aaccccggca
     6901 ctcagtattg ccgcgggcca cgacgtgaat gcccagacga gtgggcctca agtcactacc
     6961 atcatatcga agaccgatgt tgcaggaata aaagacaagg aatggaaggt ccgtaccatt
     7021 gaacttgccc ctggagcggt ggacgcacgc cttttctatt ccggtgtcga gcttgtctat
     7081 gtcctggagg gcgcagggcg cttagaggtg gatggaaagc catttgtggc attgcatccc
     7141 ggggctgtgg ctgcgctcaa tccgaaggaa atacacgttc tcaagaacac aaaccagaca
     7201 cagacgttga aagtcgtcgt cgttctcctc cgagagaaag ggcaaccgcc tctcgcgttc
     7261 gggaaccgag ggacgccctc aaaggccgtc gagcaagaca attcaacggt cccaagattg
     7321 gtattttgaa gaggctccat gcaaccgaca ggagtaggaa aagtcgcctt gtctctcctg
     7381 cgaacaatcg cgacggtgtg agccgtggac ccaatgtatt ctcaaagact ttcgattggc
     7441 gtccgcattg caccatttta gagtggtgaa tgtggtttca agctgaatgg tgagattcca
     7501 gtgatgaccc tcttcattca aaattcggca ttcagcattc agagttcagg attgctggac
     7561 agcctgaccc tgctcattaa gaccgtccag cttcggccgt acgtgtttct cttcctcgct
     7621 gcctttctct tgtccgcccc ggctctgatc gggtggcaac gaaccggtct gttcttcgcg
     7681 gtcgtctggc ttaccgcttt tgtctgtgag ttctcatcga ctcgcaccgg tcttcccttc
     7741 gggtggtatc actacaccgg ctcgacggtt ggacaagagc tctacctctc gaacgttccg
     7801 ttcatggatt ccctctcatt taccatgctc ctctatgcga gttattgcct cgcactgagt
     7861 tttcttctcc cgtctcatag tgagagaggg agaaagagcc cattttcaaa gaacgggcgg
     7921 ctcctagccc tgacacttga ccgatcggtt cgcaccggtt ggccggtgtg catgttgacc
     7981 accctcttct tcgtcttcat cgacatggtg attgatccag tagccttgcg aggagaccga
     8041 tggtttctcg gcaagatcta ctactacccc aatcccggcc tccatttcgg cgtccctctg
     8101 gctaactatg gcggctgggc agtggttggg gtgagcgccc tgctgatcta ttttgtcctt
     8161 gatcgtcgcc tgcccgacct tgagctgcgt tcacatgagt ctgttacgag caacattctt
     8221 atgggctgca gcctctatta tggtgtcctg cttttcaacc tcactgtcac attctggatt
     8281 gatgaaccgt tgataggcat gaccggcatc ctgatctaca ttcccctcac ggtgctgttg
     8341 ctcttgcggc tgcttggcaa actccctgct cccgcaaccc tgtccccgcc tgatcaccgg
     8401 atggtggacg ggtcacggct ggttaccgtg ctacatcgat cccacaagca cggcccctca
     8461 tcttggcgtg aggcgggatc ggaatggctg gagtccagcg cgcatcacag gggtgacagg
     8521 ctccttggtc aagacgcgag aactctcgat acgatcggca ggagtgggtg atggcagttc
     8581 cgctgtccca gatggtcacg gtcgcgcaat acgttctgac ccaacagctg aagggcgtcc
     8641 aacggtaccc cctggtcctg atgctcgagc cgttgttccg ttgcaacctg gcctgtgcgg
     8701 gctgcggaaa gattcagtac cccaatcacg tcctcgacac acgcctgacg ccagaacaat
     8761 gctgggctgc ggtcgatgaa tgcggggctc cgatggtcag cattcccggc ggggaaccgt
     8821 tgatccatcc ggagatgccg gagatcgtcg gcgggatcgt cgcgcgcaag aaatatgtgt
     8881 acctctgcac caacgccatt ttactggagc ggaagctcga ccagtacgaa ccgtcgaaat
     8941 atctcacctt cagcattcac ctggatgggc tccgggagga ccatgaccat gccgtctgcc
     9001 gggacggggt ctacgacgtg gcggtgcgcg caatcaaagc cgcgctcgaa cgaggccatc
     9061 gggtcaccac caatacgacc ctgttcagtg atgcaaaccc cctgcgagtt cgcaggttct
     9121 tcgacgagat gatggtcctc ggcgtcgaag ggatgatgat ttccccgggc tacagttaca
     9181 cgaaagcgcc ggatcaggag cactttctcc ggcgacagaa gactcatatg ctgtttgccg
     9241 acattctcag taaccgcaag cggtcatggc gattcaatca gtccccactg tttctggagt
     9301 tcctgatggg gaagctggac taccagtgca cgccctgggg aaacccaacg tacaacatct
     9361 tcggttggca gaagccgtgc tatttgctgc aggacggcta tgtgaggacc ttccgggaac
     9421 tcctcgacgg cacggactgg gatcgctatg gtaccgcacg gaacgagaag tgcgccgact
     9481 gcatggtgca ctgcggctac gaggcttccg cggtcaccga tactttcgga tcctggggag
     9541 gattcggccg cacggtctgg gcaatgctcg ctcccaatac acgctggcgc aaacgccctc
     9601 cttctatcct tcacgtgtga ctccatagcc gattcgaaga accatattcc ggatgcgact
     9661 tcgctccaag ggtagttgtt ccaccctgcc tcgccggccg agttgatcga agcttcggag
     9721 cacgcattag attaccgtgg cgatgtcctg ctcgaatgcc gggggataca tcaagtaatt
     9781 gcttggtcca gttatttttt gatcggggtt cgatgtggag gccagtacct cgcacatgcg
     9841 gggggcgacg atgtttcggc acttgccgag atgtcggcag tatctccccg tcaccgccct
     9901 ctcatcgcca gcgattggtt cctcctcgcg aagcgagttc ttctctattt ttcctcactt
     9961 ccgcaatcta tggaggaaga tagcttgtgt ggaacgatca ttgccatgag aaggactgcg
    10021 agccacacaa tcatccacaa tcttcgatgt agacgaggcc ggtatgaacg aagctcttca
    10081 ctttctgacg caacatggaa ccctggtcct cttcgcggct gtcttcgcgg agcaaatcgg
    10141 gctaccgctg cccgctgtcc ctttcctaat cgcagctggt gccttggcgg gcaccggaca
    10201 aatgacgttg ggtatggcag tggggtcagc agtccttgcg gctctcgcgg ctgaccaagt
    10261 gtggttcgag ctcggccgac ggcgaggccg ccgggtcttg aactggctct gccgtatctc
    10321 gctcgagccg acctcctgtg tccgtcggac cgaggaattc tttgcccgtc acggagtgcg
    10381 ttcgctggta gtcgcaaaat ttatcccggg tctgagcacg attgcgccgc cgcttgccgg
    10441 gattgtgggg ctccgtgtgc cgcagtacct cttgttcaat ggcttgggaa cgatcctatg
    10501 ggtgggttcc ggcatcggtc tcggctatgt gttcagcgac caacttgagc gaacggtatc
    10561 tgtcactgcc cacgtgggtc ccacagtcgg actggtcctc cttggaggtg taattggtta
    10621 tgttatctat aaggctctac accggtatcg tatggaacgt cttgtgccgc ggatgaacgc
    10681 tcaacaagtg agtgagaaaa ttgcggcagg agaagacccc ctcatcatcg acctgcgtcc
    10741 cgttggcgat cgtcatgata ttccgggaat accaggctcg ctcacccggt cattggaaga
    10801 attgatcgcc aggcaccacg atcttccgcg ggatcgcgac gtgattctct attgcgcgtg
    10861 cccgaaggat gcggcaagcg tccaggcggc gtggaagctc cgcgaaaagg gattgacgcg
    10921 tgtttggccg ctcgccggcg gcatcgaggc ctggcacgcc atcgccctcg agagtggggg
    10981 ggcgataaga gttaccgaag ggcacacgat cgcagtctga ccggtcaagt gcgggtcgag
    11041 ctgattttct catcacaagc gattattgct ggctagacga caaccaatgg aaagaaggag
    11101 agatgaacca tgagttcttt aacgaaaaaa gtggcgatcg taactgggtc gtcagacggc
    11161 atcgggcgcg cgattgcgga gcggttggcg caggacggag cgaccgtggt tgtgaactac
    11221 ggtaagagtg cggacaaggc caagcaggtc gtcgccggca tcgaatccaa aggcggcaag
    11281 gcggtcgcag tccaagccga tatgagcaag gtcggcgatg tcggacggtt gatcaaggat
    11341 acggtgaaga cattcggcgg gctggatatc ctggtcaata atgccgggat gttcatgttc
    11401 aagcctctgg ctgagacgac cgaggaagag ttcgaccgta tgttcgccct caataccaag
    11461 ggcccgtatt ttgcgcttca ggaagcggcg aaggtcatca aggaaggcgg ccgcatcgtg
    11521 aacatttcga cggacggcac ccatattggt tttgccggcg ctaccgcgta cctcggcagc
    11581 aagggggcgc tcgaacagtt cacgaaaggc ttggcccacg agctggcgcc caagggggtc
    11641 acggtgaata ccgtatcccc cggctataca atcacggcga tgcttcccgc cgatcccgcc
    11701 ttccgccaga tcggggagca ggcgtcgccg ctcaagcggc tcggcacacc gaaggacatt
    11761 gccgatgttg tggcctttgt cgtgagcgaa gaggcgcggt ggttgaccgg ccagaatatt
    11821 catgccggcg gtggcgtggt gatgtaatcg gcgggaggca actcatgaaa gaaaacctag
    11881 aagaagtaaa actgttggcc cgcgatcttc ggaacggcaa agagtttcct cgtagcccac
    11941 gcgaaacgct ggcaggctat gtgctcgctg cccgtgcagt cgacaaatgc cgcgccgtcc
    12001 tcgttggctg gcagggcgag taccactcta actgtccgct cgatcagcgg tggctgaaat
    12061 tcgccgagat cgactacggt gcgttcaggc ggtttgtggc cacgggtgcg actgacgata
    12121 agatcgccgc gtggattgtt gagcatgcca agaaacgctc tcgcgccgac atcgtcgtct
    12181 ggaacaataa ggaacgcgac cttagcttga gcgatctgac gccggaactg caggaataca
    12241 tggaggacta catcgcgtcc tgcgtcccgc gccaccgtgt cgtgtaccgt tggttcgacg
    12301 tgtatgactt ggaagagcaa cgtctttagg gggatcgagc cgaccttgca ggagctcagc
    12361 cgaaccccct tcgtcatctg ataagccttg cggctgtgga gaaggaggac aggccatgct
    12421 accaacacgc ggatatgcaa ccaagagccc gacgtcgatt cttgaaccat tcaacttcga
    12481 gcgacgcgac cctgggcccc gcgacgtcct catcgagatc ttgtactgcg gagtctgtca
    12541 ctccgacatt catcaggcaa gggacgagtg gggcggctcg atcttcccga tggttcccgg
    12601 ccatgaaatc gtcgggcgcg tagcccgcgt cggatcccag gtgaccagat tcaaggtcgg
    12661 tgagctggcg ggcgtcggca cagtggtgga ttcctgccga gtgtgcgaga gctgcaaaga
    12721 aggcctggag cagtactgcg agaaacaccc ctcatggacg tacaacggaa ccgagcagga
    12781 ccaaaagact ccgacatacg gcgggtactc ttctcacatc gtggtggatg atcattacac
    12841 gttcaagctt tcgccgaatc tcccgctcgc gaacgtcgcc cccctcctct gcgccggcat
    12901 caccacctat tcaccgctcc gccatttcaa tgtcggtccg ggccagaagg tcgggatcgt
    12961 cgggctgggc ggattgggcc acatggcggt caagctggct gcttcaatgg gcgcggaggt
    13021 caccgtcttc agcacgtcca aaacgaagga acaggatgcg cggcgcctgg gggcgcatga
    13081 tttcgtcgtg tcgctggacc cgaagaactt cgccgccctc gccaaccggt ttcatttcat
    13141 ccttgacgcg gtctccgcgc cgcacgacgt caatgcgtac ctgaatcttc tacgcaggga
    13201 tggggtactg actatggtgg gagttccggc caagccattg gagatccagg ccttctcgct
    13261 catcctcaag cgaagaaaac tggcgggctc gggcgtcggc ggcattcgcc agacccagga
    13321 gatgctcgac tactgcgcgc aacacaacat cacggcggac gtcgaggtga ttccaatcaa
    13381 gcaagtcaat gaggcctatg accggacgct caaaggcgat gtgcggtatc gcttcgtcat
    13441 cgacatgaaa acgctgtaac acctcctcac cctaccctct cccccgctgg gggagaagag
    13501 aaggtgaggg agaaaggaac caccgcgatg catttcatga ccatggccaa ggcgacgaac
    13561 gatctgcaag cgggcgtcat gccggaggag aagctcactg ccgagaaggc gacgcaccag
    13621 gaggagccgg ggaagaccgg cgtgcaactc gatgcctccg ggctccagcc aagttcggag
    13681 ggttcgcgca tgaaagactc gggaggcagg cgtatggtga tcgatggacc gttcgcggag
    13741 acgaaagagg tgatgggcgg gcacatgctg acccaggcga aatagtagaa agagacgacc
    13801 gaatcgacct tttcctcggt ggaacaagga ggtgccgtat gccgttaggt gtaacgtgga
    13861 cgattgtgtc gtcgcttgtc ttgatcgcat gctcggtgtg ggcactgatc acgttttatg
    13921 cgcggggctc ctcgctcgat acggcggcgc ggcggcgttt tggtatcgct tcgggaatgt
    13981 atctttttgg gtggctggtg cttgcgtttg tgctcgggag caacgggata ttccaagcga
    14041 ccccctcgag agcgtttcca gcactggcgc ttggtatcgc actgctgatc ctcacgggtg
    14101 cgtggctgct gtatcgatct tcggcgttga agaccgtact tgcaacggtc ccgctgccgt
    14161 ggctggtggg tgtccagctc tatcgggtgc tgggactcaa tttcctggtt ctgtactctc
    14221 tcggtcgcct gcccggcgag tttgcaattc cggccggctg gggggacata gccgtgggtc
    14281 ttgccgctcc ggttgtcggt tacttgctct acaaaggcta ccgctggtca tgtctcgcag
    14341 cattgaggtg gaatgtggtc gggattctcg acttcgtcgt ggcggtgacc acggggtttc
    14401 tatcctcacc ggggccgttc caggcgcttg ccttggagaa ccccaacatg ttgatcacgg
    14461 cgtttccgtt ggtactggtg ccgctctatg ccgtgccatt gtcgatccta ttgcacctgg
    14521 ctgttctgaa gcgcctcaag gttacgctgg gggcgccatc tgacaaggag ctcgagtgcg
    14581 gcagccagtc ggggcactgg ggtttgcacc cggtcgcaca ttaacttcga agagagaatg
    14641 cctggcgagg ggaagcgctc aatctaagga ggcgcgatga cggataacgc gagggtcacg
    14701 gaaatcgccc ccgatcactt tgcgatttcc atctacgtca aagaattcaa cctgcggttc
    14761 aaccacttcc tgatcaagga cgaggagccg ctcctgtttc acacaggaat gaagcagatg
    14821 tttccattcg tgcgggatgc ggtcgcgcgc gtcatcgatc cttccatgct gcgatggatc
    14881 tcattcagcc atttcgaagc ggacgaatgt ggagcgctga atgactggct ggccctggcg
    14941 ccgcacgcgg aacccatatg cggcctggtc ggggcgctgg tcagcgtgaa cgacttttct
    15001 attcgtcagg cgcacgtgct tgcgcacgac cagatactca aaactgggaa gtaccgtttt
    15061 cgatatcgcc agacgcccca tgtgccacat aactgggagg cgggcctgct gttcgaagaa
    15121 gtcacgcgta cattgctgtg ctcggatctg ttctctcatg aaggagacgt ggaaccggtc
    15181 actgagtcgg atgtagtcga acgagccagg cacgcgttga tcgaaggtca aaaaggatct
    15241 ttcgccaacg cctatcccta cacgccgttg actgagccga ttctgcatgg actggcggat
    15301 ctccggccaa aacaattggc gctcatgcat gggtcctcgt tcgtcggaga tggcgagcga
    15361 gcgctgcggg acctggcgac cgtcatgcgt gaagtgctcg gagcgagcac tgcgagacga
    15421 tgaccactct gcttggtgga tgtgatggcg atcgagctga accacacgat cgtgccggcg
    15481 aaagataagg tcgcgtcggc aaagttcttc gcgatgatgt tcggactatc gttcgatgag
    15541 aacgcggtcg ggtatttcgc ccccgtgaag gtgaacgaga cgctcacgct cgatttccac
    15601 gacgacgtcg accggttcga ggtccatcac tacgcgttca aggtgagcga accggaattc
    15661 gatgccattt tcacgcgcgt gcaagaggcc ggcatcccct tcggcagggg gccggggaat
    15721 ctggaggacg ggaagctgaa ttcatcgaac ggtggccggg gcgtctattt ccgcgatccc
    15781 aacggacaca ttctcgaact gctcacggta gggtaacgcg acgatcgggc ttctggcttc
    15841 tttggcctct cggacctcgc cctgtttcgt gctctgcgcc atcagctctt ggtttcgaac
    15901 ctcagcactc atgactcaac acttccgagc gccataagct atacgccata cgctctgccc
    15961 tcttccgtat ctgcctatga atcgccagat cctcatcgtt agtgcccacc tgaagccagg
    16021 cattacggaa accccacgca ggtctctcgc acgacagttc agcggaacac tcgacagctg
    16081 cgcggttgtg ccgcagctga attctggccc gggtcacgac ctagatcagt accttgaatt
    16141 gaccgcggga cccccttcgt gcatccgccg atggttcggc gtgtgccggg atatcggcag
    16201 agctcgatca tcaggagatc ggcggagtcg gaaggactta agactcgctt tgttctttca
    16261 atggcttaga cgatcgtttc cgatggtcgt cgctggaacg gcccttgcca atccaaaaga
    16321 tgagggggaa caaggaatgc tgaagatcac caaacatcga gatgccgggt cggactcagt
    16381 gtcgttcaaa cttgagggac gactcgcagg tccttgggta ggggaactcg acacctgctg
    16441 gcgtcagatg acagcgagtc cacaaggccg caccgtggtc gacttgaccg gcgtgacctt
    16501 cgtggacggt gaggggaaag cgcttttgac gcgaatgtgg caacagggtg tagagcttcg
    16561 cgcggccggt tgtctgacca gatgcattgt ggaagagatt gcgaaaactg gacaggccga
    16621 tgcatctcgc tcgcgtcgga aggataagta ggagggaagc tgagtgtggt cacggctttg
    16681 attggttgtg ttgatgaccc tcatcttccc gttgctgcgc gcggcgttac tgcttatgta
    16741 ccttgttgag cttgagagta aaccgctaca atgatcgtcc ggcaagggtg atcagaaaga
    16801 atgccgatcg caaaatcagt cgaacggaga aagccgccat gtctttaatc agtcactcat
    16861 atcgagtcgc tgcgatcttt ctcgtcggtg taggagcagc gagcgtgtgg gggtgcaaac
    16921 aagaggcggc ctccacaccg gcccgcccta tcccggaggt gccggtggtg gccgcaacgg
    16981 tccaaaatgt gcctgatgag ccggagttca tcggtcaaac tgagtcttca cgtccggtgg
    17041 agattcggtc tcaggtgaca ggaatcatca aggagcggtt ctttgccgag ggccgtgcag
    17101 tcaaaaaagg tgatcgtctc tatcaaatcg acccggttcc gttcaaagca gccttgctca
    17161 gcgcgaaagc gaaggtggcc caggctgaag cgcgactggt gcaggcgaag caaaaccttg
    17221 ctcgcgtgaa gccccttctg gcagagcaag ccgtgagcca gaaggacgtt gatgatgcag
    17281 tagcagaaga gttggcggca gtggcggcgc tggacggggc gaaggccgat ctggtcaagg
    17341 cgaagttcga ctttgataat acttttatca tcgctccgat taacgggatg attgaacgga
    17401 ctcgagttta cgaaggacgc ttggtttcgg cccagaccga tctcctcacg atcgttcatc
//
LOCUS       DATIFR010000179         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1455659, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000179 DATIFR010000000
VERSION     DATIFR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455659"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..923
                     /locus_tag="VLD60_10340"
     CDS             213..923
                     /locus_tag="VLD60_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSB45402.1"
                     /translation="MQQQVCDDRHPGITTILAMLRKIDGLRLSLASRRDVELILVRQV
                     SRQFDHALPWQAGHGLRTAVTSSLIGQAIGLSAHALHDLELASLLHDIGLLMLPHHVQ
                     KGHTPIEAASYVAVQNHPRIGASLLEPFPFLREASILIAHHHERWDGSGYPYGIRGPF
                     IPLGARILAVADAYDAIKVPGTSDPAVRNLIALRILRVASGTQFDPALVEVWGMIQQS
                     LSGSDLSESVNGCCVDPR"
     gene            1108..>2585
                     /locus_tag="VLD60_10345"
     CDS             1108..>2585
                     /locus_tag="VLD60_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45403.1"
                     /translation="MGERLFVETRFAQSFKAFLDSGGNVNDHNAGDAVMDTLETTTPG
                     APIDPGPFKGMSMNCRACHMVDDVLTASGGGMRTYADFARRSPIPARTDGKTHAPRNS
                     PPLVNSALDRPGGSLFHFDAEFNSMEDLVAATFTGRNYGWLHTERSQAIAHIARVVRG
                     DDGSGVIAQQFDGLPYRILFTGGNPAIPDELRLPPEFRAFIGSGTDQEIFDAVVKVVA
                     AYVKQLLFSQQDDSGNLIRSPFDVFLSLNGLPQQPNNNESALDYSRRLRTLVNGLASP
                     QFVSSNPNRPDGQFQFHSQSFTFGPQELEGLKIFLAEPSAVTPSAPELAAGKIGNCPV
                     CHAAPNFTDFKLHNTGTAQTEYDSIHGAGAFAALAIPNLATRNGNYDAFLPATEIHPN
                     ASERFRAIPVLADPSLTDLGVWNVFANPDIPNPQAKIRTILCDEQQPCPFSDAVLLDR
                     AIARFKTPGLRDLSHSAPYMHNGQFDTLDDIVGFYRGISTQAR"
BASE COUNT          560 a          802 c          650 g          573 t
ORIGIN      
        1 agacaccctg atgatgggaa gttttttcgt gatgatgacg attccacttt acgagatgtg
       61 ggtcttgaaa cagcctctga tggcgggaaa gcggagcgaa ggctcctgat atagtccgac
      121 agtcgaacag cctattccgg ccagaggttt ttcacccaga gcccaagatc acatgatgtg
      181 gtcttgggct cgttcttttt cagcagaagc cgatgcaaca gcaggtctgt gacgatcgtc
      241 accctggcat tactacaatc ctggccatgc tcagaaagat cgatggcctg cgactttctc
      301 tcgcatcacg tcgtgatgtc gagctcatac tggtacgaca agtgagcagg cagttcgacc
      361 atgccctccc gtggcaagcc ggtcatggcc tccggacggc tgttacctca tcgttgatcg
      421 gtcaagcaat cggcctttcc gctcacgcgt tgcatgacct tgaattggcc tcattgctgc
      481 acgacattgg actcttgatg cttccacacc atgtacagaa aggccacaca cctattgagg
      541 ccgcgtccta cgtcgccgtg cagaatcatc ctcggattgg cgcgtcgctg ctggagccct
      601 tccccttcct ccgggaagcc tcgatcctga ttgctcacca ccatgaacgc tgggacggat
      661 ccggctatcc gtacgggatt agaggaccgt ttatccctct cggagccagg attctcgccg
      721 tcgccgatgc ctacgacgcc atcaaagtcc ccggcacatc agaccctgcc gtccgaaatc
      781 tcattgcgct cagaattctc agggttgcct ccggcactca attcgatccg gcactcgtcg
      841 aggtatgggg catgattcaa caatctcttt ccggttccga tctcagcgaa tcggtcaatg
      901 gatgctgtgt agatccacgt tagacgtatt ggaggctgca ccatgttttc tcttcgttcc
      961 tgtcacaaca cataccctca agctgtcgga gggtcatcac tgatcttcat ctctctgctc
     1021 tgcgtcacag tcggaggatg cggtagcagt ggtagttccg caccatcctc agtctcggct
     1081 acaggaacac cgggggccga agcctccgtg ggagaacgcc tcttcgtcga aacccgtttt
     1141 gcccaatcgt tcaaagcatt tttggatagc ggcggtaatg tgaacgatca caatgccggt
     1201 gacgccgtga tggataccct ggaaacaacc acccctggcg cacccattga tccaggccca
     1261 ttcaaaggca tgtctatgaa ttgcagagcc tgtcacatgg ttgacgatgt tttgacggcc
     1321 tcggggggag ggatgcgcac ctatgcggac tttgcccgcc gcagcccaat cccagccaga
     1381 accgacggca agacccatgc gccgcgcaat tcgcctcccc tcgtcaactc cgcactcgat
     1441 cgaccaggcg gatccctctt ccacttcgat gcggaattca actcgatgga agatctcgtc
     1501 gcagcaacct ttactggcag aaactacgga tggttgcaca ccgaacgatc gcaagcaatt
     1561 gcgcacatcg cgcgggtcgt acggggcgat gacgggagcg gagtgattgc ccagcaattc
     1621 gacggactcc cttatcgtat tctctttacg ggagggaacc ctgccattcc agacgaactg
     1681 cgcctgccgc ctgaatttcg tgcgttcatc ggatccggta ccgatcagga gatcttcgac
     1741 gcggtcgtca aagtggttgc cgcctatgtg aaacagcttc tgttttcgca acaggacgac
     1801 agcggcaatc tcatccggtc acccttcgac gtgtttttga gcctcaacgg cctcccccag
     1861 caaccgaaca acaatgaatc agccctggat tacagccgca ggctgcgaac gttggtcaac
     1921 gggttggcaa gcccgcaatt tgtctcgtcc aatcccaaca ggccagacgg tcaatttcaa
     1981 tttcattccc aatcgttcac attcggccct caagaactgg aaggcctgaa gatctttctg
     2041 gccgagccat cggcagtcac gcccagcgca ccggagttgg ccgcaggcaa gatcggcaat
     2101 tgcccagtct gccacgccgc gccgaacttc acagacttca agttgcacaa cacgggtacc
     2161 gcacagacgg aatatgacag catccacgga gccggcgcat tcgcggcact cgcaattccc
     2221 aaccttgcta ctcgtaacgg aaactatgat gcctttctcc ccgccaccga aattcatccc
     2281 aacgcctcag aacgttttcg cgcaattccc gtcctggctg acccctcctt gactgatctg
     2341 ggcgtgtgga acgtcttcgc caatccggat attccgaatc cgcaagcaaa gatccgaacc
     2401 attctgtgcg acgagcaaca gccttgtccc ttctccgacg cggtattgct ggaccgagcc
     2461 atcgcccgat tcaagacgcc gggactccgc gatctgagcc attcggcgcc ctacatgcac
     2521 aacggccaat tcgatacatt ggatgacatc gttggcttct atcgtggaat ctcaacacag
     2581 gcccg
//
LOCUS       DATIFR010000180        11576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1267158, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000180 DATIFR010000000
VERSION     DATIFR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267158"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VLD60_10350"
     CDS             complement(<1..452)
                     /locus_tag="VLD60_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSB45404.1"
                     /translation="MPKRRCYWLMKSEPSVFSIDDLAKSPKQTTSWDGVRNYQARNFM
                     RAMAVGDLVLFYHSNAEPPAIVGTAEVVRTVYADDTQFDKRSHHYDAASTKAAPRWDM
                     VDIKYRERFKRSLSLDRLRQEPKLKGMVLLRKGSRLSVQPVTESEWAV"
     gene            complement(772..1971)
                     /locus_tag="VLD60_10355"
     CDS             complement(772..1971)
                     /locus_tag="VLD60_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSB45405.1"
                     /translation="MTGLLLKIQGLAAVALIAMLPAWWPDEATGTDGTIGRPSEAAFD
                     LEPVVQKGLQSPLFVTHAGDGSGQLFVVEQGGTIRVIDRDVLQEAPFLDLRDRVTTKG
                     EEQGLLGLAFHPDHRRNGRLFVNYNRRDDGATVLAEYSRQGRSLQISAATERILMVVP
                     QPYLNHNGGMVAFGPEGYLYIGRGDGGSRGDPQNRAQNPHEWLGKILRIDVDRERPYA
                     IPRDNPFVKGGGRQEVFAFGIRNPWRFSFDRETGMLWLADVGQHKWEEVDLVVAGGNY
                     GWRIMEGTHCYKPEQDCNPEGLIFPLAEYGHEQGRCSITGGYVYRGQAVPALRGTYLF
                     GDYCSGELFALSSSANRKASTVPRVLMHTGYRISSFGEDEAGEVYVVDHKGGLYRLAP
                     SRSRHTP"
     gene            complement(1968..2333)
                     /locus_tag="VLD60_10360"
     CDS             complement(1968..2333)
                     /locus_tag="VLD60_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45406.1"
                     /translation="MALSGHVVGLLKEYMRDLVEQAEQEATAHESFGFSTMPYRSDQA
                     ISDLLAILDDRIESEGLQVGLPEDFLHQMWSLCNEARVQVTDQVWLDRNSRDQPASKA
                     RVREMTYQALITFLETHET"
     gene            2452..3252
                     /locus_tag="VLD60_10365"
     CDS             2452..3252
                     /locus_tag="VLD60_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSB45407.1"
                     /translation="MAMPSVERFEIALNTPAGRLTTTLDVPTGFVPITAIVPVARRLG
                     EEAVQLEERHAKETGQAISCRMGCAACCRMMVPVSPPEAFSLRDYVEQLPADQRDRIS
                     TRLAETKNVLQKQGLLTRLHDVANASKALPDEELEPVNRAYYALRLPCPFLENEMCSI
                     YEARPAACRELLVTSPAELCSDIVGNPVRPLPVPIRIGTVLGLVWASLTQSTPRLIPL
                     PLALDWADRHRQESAQTWTGSGLIDQMLDKMWRFLSQEFHARGQTPQG"
     gene            3306..3926
                     /gene="thrH"
                     /locus_tag="VLD60_10370"
     CDS             3306..3926
                     /gene="thrH"
                     /locus_tag="VLD60_10370"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HSB45408.1"
                     /translation="MQKPVIACLDLEGVLVPEIWINVALKTGIEELKITTREMPDYDA
                     LMKRRLAILDQHRLTIADIQAVIDKMGPLEGAPAFVSWLRERCQLIILSDTFYQFALP
                     LMRQLGFPTLFCNQLEIDGSGRIANYHMRMQNQKKHSVAAFKSLNFFTIAAGDAYNDT
                     AMLSEADAGFFFRPPDYLPKEFPQFPVTQTYKELQHRFSQAGNLRP"
     gene            complement(4322..6004)
                     /locus_tag="VLD60_10375"
     CDS             complement(4322..6004)
                     /locus_tag="VLD60_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSB45409.1"
                     /translation="MKSILGRTGWASVATFFSVAVMAAGMSFVTVPAQAAFELPEGEK
                     ITNVPAIPRAIPQKEAYELYDPKIGKNFDIKNFWLRADLRVRPEMRNQGCFGSTSVGG
                     GACNAFGGNSANGTKGKSNDFFVQQWVRLGIGYDLSPDVNFYMEIIDSAVWGANGANG
                     TGGAQVDALNHNGCSTNNGGGNCGRLGVRAAYMLIRNFAGIQGFSVKAGRQYLVFGNH
                     SLFGHFDWANTGFSHDGVMLQYSTKAWDSYLGWFRTSESDLGQASPVGSLAPNIGPSA
                     INNGSANTDSDMLIFYNQIKSVPGFLIEPYYILYSNRYNTIDNRGQGVGTAKHANQTR
                     HNVGNRIEMRKGNFDATNETVYQFGEMGDNSAGALAAQGNQKNLHISAWATRNWIGYT
                     AYQSAWKPRLAFNFDYASGDGRANCTLAGSTTPCKTANTFENLFPTNHIHMGYMDVQA
                     WKNMLSPSVNFQARPTKDDHIEVWYTNLNLANAKDNWYRGAQGVYVFSKNGNTKKHIG
                     DEIDFTWTHMFMDGKLAFQATYGHLFAGGYIQENLGTSVDQEWAYAQLWINF"
     gene            complement(6352..6651)
                     /locus_tag="VLD60_10380"
     CDS             complement(6352..6651)
                     /locus_tag="VLD60_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSB45410.1"
                     /translation="MFGLGAGEILIILVIAFLLFGPKQLPEVGRQVGKAVKGFKETAE
                     DLRKSVEPELNMIQQEVKMVEQDFQASMKEAEEEINQATTPSDKQAGSSGPANQA"
     gene            complement(6748..7017)
                     /gene="tatA"
                     /locus_tag="VLD60_10385"
     CDS             complement(6748..7017)
                     /gene="tatA"
                     /locus_tag="VLD60_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSB45411.1"
                     /translation="MFGSFGWMELLLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     NEADAIDVTPPPADAAAPVQPQAAQGQTPPEAAAQTQQGQQAKQG"
     gene            complement(7199..8680)
                     /locus_tag="VLD60_10390"
     CDS             complement(7199..8680)
                     /locus_tag="VLD60_10390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45412.1"
                     /translation="MLQDADALPQLIGDYKPVDQWQVHINQLFYRLRGDQIRRYYQTF
                     ASADHRLAHALATDYYEKVITREKKRGKGATGQRGSAESGSTDTPSISLSPASPLTVL
                     ELGPGNGNLAACFLTHLKTLDRDGLIYPRVRYVLVDWEQTMLDAATAHPVLAAHRDRV
                     ETLCSVVDQVAGVADGTVDRIFCNELWNDLPTKLMSRQAGEIEEEFLRPNLSEALHAK
                     IQDWATFVRAFEAKDVETLKTFPPFFDDLVWEREYRAVEWKEVPYRKTITEFLRRIDE
                     QVVVPVNIGAHATIKEAKRLLAPDAVGFSSFDAGTADLKVLNDPDKPCSGQFGGQQSF
                     MVNFTLAEAVVKQLEAGTVSIESQREFIGRSLGTNVLTLMDLLATHPLAGSKMEPWEQ
                     DRLILKTIKALNETYQSPYARSMEFPIRADMPTEERDMLQALVRELKPGGVPDTVAYI
                     TEEELTLSSKDLEDVGYDTQSFLIALTAPPSPVDYFHLSIRPR"
     gene            complement(8708..9079)
                     /locus_tag="VLD60_10395"
     CDS             complement(8708..9079)
                     /locus_tag="VLD60_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSB45413.1"
                     /translation="MGRKGFGDLLVWQKAKDLAVKIYHISDRTELRKGFGLRDQMRRS
                     AISVASNLAEGDERGTDREAIRFFYMAKGSVTELRTPFQIAYDVGYVERSTYDSVEVE
                     CVQLGKMIGSLIRARNQATCL"
     gene            9319..9876
                     /locus_tag="VLD60_10400"
     CDS             9319..9876
                     /locus_tag="VLD60_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45414.1"
                     /translation="MRLSVVVSLTLCSCLLGGALVQAGDLPITKNPPIPEFQNRLQDA
                     KPYDFDAPPQGMFRAITMAEGFDEELGFRRTHEIVPVKPTEQFRPDAAGVFIVFQLHQ
                     HYQAFQVFGRCFPESVPGLDPRTIVGQDAMYIALEDESGYLKLAPPEGGWKPGRYKVE
                     IHAGEQINEMSLMGTMRFTVLAEKQ"
     gene            10019..10765
                     /locus_tag="VLD60_10405"
     CDS             10019..10765
                     /locus_tag="VLD60_10405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB45415.1"
                     /translation="MTDAPRPWASVVIPIKDERDNLAPLTERVLKVLGARNESRSAPY
                     ELIYIDDGSTDGSSELLDELARNYPPVRVLHFDRNYGQSSAFDAGFKHSTGALVITMD
                     GDLQNDPVDIETLLPLTQSYDLVCGWRTTRNDNLVRKLSSRIANAVRSAITGDRVHDT
                     GCSLKIFRRAVVDKLQLFTGMHRFFPALALMHGFTVTEMPVSHYPRAHGISKYGVGNR
                     LFKGLYDLIAVRWMQHRVLRYQYRHPSLHS"
     gene            10805..11104
                     /locus_tag="VLD60_10410"
     CDS             10805..11104
                     /locus_tag="VLD60_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HSB45416.1"
                     /translation="MTETIWIAIGFLGQGLFFGRWVLQWIVSERKAKSQVPLAFWYMS
                     LIGGLITLAYAIYRKDPVFIAGQGVGAVVYIRNLVLIRRAGTSPTTNPAQSAGNS"
     gene            11149..>11576
                     /locus_tag="VLD60_10415"
     CDS             11149..>11576
                     /locus_tag="VLD60_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HSB45417.1"
                     /translation="MPTSKYVVNLAVLLSLAILLLFVGLGSIGLTDRDEGRNAEAGRE
                     MLETGDWMTPTFNYEPRFYKPAFLYWLMSGSYATFGVDEFAARFPSALFGAALILLQY
                     WFLSRYRNAQIGLFGALMLLLNIEMIGLSRMALTDSVLVF"
BASE COUNT         2371 a         3502 c         3063 g         2640 t
ORIGIN      
        1 atgactgccc attcggattc cgtcaccggt tgcacggaca gccgggagcc ctttcgtaat
       61 aacaccatac ctttcagctt gggttcctgt cgaagtcgat ccagagacag gctacgtttg
      121 aatctctccc gatacttgat atcgaccata tcccatcgcg gagccgcttt ggtactcgcg
      181 gcatcatagt gatggcttcg cttgtcaaat tgcgtgtcgt cggcatagac cgtccgcaca
      241 acctccgccg tacccacgat ggcaggcggc tcggcattac tatggtagaa caacaccaga
      301 tcgccgacag ccatggcgcg catgaaattt ctcgcctggt agttccgcac tccatcccag
      361 ctggtggttt gtttgggtga tttggcgaga tcatcgatcg agaatacgct cggctctgat
      421 ttcatgagcc aatagcatcg ccgtttgggc atctgtttct cccgtgagac ttggccatac
      481 caaaagacgg ctgtatgcag tcgcccgtta taccccaaga taaggtcatg tgatggaatt
      541 cgcgccattc tggtttggaa tttataagct cgtcaagtac ggactccact ccctgaactg
      601 ggccctttat gatttcgtcc tgagaaacaa gccagaagac agctggagcc agatagctcc
      661 attcgatttt tccccccagt gacttggcca ttcaagatac gagaggatgg gtggcggaag
      721 tggccgggat agcgctctgt tggatccatg gaaaacttta aatagaggga tctatggtgt
      781 atgtcgcgac cgacttggag ccagccgata gaggccaccc ttgtgatcga cgacatacac
      841 ttcaccggct tcatcttcgc cgaatgaaga gatccgatat cccgtatgca tgagcacccg
      901 cggtaccgtc gatgccttcc ggttcgctga ggaggaaagc gcaaacaact ccccgctgca
      961 gtaatctccg aagagatagg tgccccgtag ggccggcacc gcctgcccgc ggtacacata
     1021 gccgccggtg atcgaacaac ggccctgctc atgtccatat tcggcaagcg gaaagatcag
     1081 cccttccgga ttgcagtcct gctcgggctt gtaacaatga gttccttcca tgatccgcca
     1141 gccgtaattt cctccggcga cgacgagatc cacctcttcc cacttatgct ggcccacatc
     1201 ggctaaccag agcatccccg tctcccgatc aaatgaaaac cgccagggat ttctgatgcc
     1261 gaatgcaaac acttcctggc gaccgccgcc tttcacgaag ggattatcac gggggatcgc
     1321 atagggacgt tctcgatcga cgtcgatgcg taggatcttc cccagccatt catgcgggtt
     1381 ctgtgcgcga ttttgtggat cgccgcgcga accgccgtcg ccacggccga tatacaggta
     1441 cccctccggg ccaaatgcca ccatcccgcc gttatggttg agatagggtt gcggcaccac
     1501 catgagaatg cgttccgtcg cagcagaaat ctgtagactc cggccctggc ggctgtattc
     1561 cgccaacacc gttgccccat cgtctcgccg gttgtagttt acaaacaacc ggccgttgcg
     1621 ccgatggtcc ggatgaaacg ccagtcccaa gagaccctgc tcctcgccct tcgtcgtcac
     1681 gcgatcgcgc aggtcgagaa acggagcctc ctgcaggacg tcccggtcga tcacacgaat
     1741 cgtcccgccc tgttcgacaa caaatagttg ccctgaccca tcgcccgcat gggtgacgaa
     1801 gagcggggac tgcaagccct tctggacaac cggctcgaga tcgaacgctg cctccgacgg
     1861 ccggcctatc gtcccatccg tgcctgtcgc ttcatccggc caccatgcag gcaacatcgc
     1921 aatcagagcg actgccgcca atccttgtat cttgagcagc aagccggtca tgtctcgtgc
     1981 gtttcaagga atgtgatcaa tgcctggtag gtcatttcgc gcaccctggc cttggaggcc
     2041 ggttggtcac ggctattccg atccagccac acctggtcgg tcacctggac ccgcgcctcg
     2101 ttgcagagac tccacatctg gtggaggaaa tcttccggca accccacctg cagcccttcc
     2161 gactcaatcc gatcatccag tattgctaat agatctgaaa tcgcctggtc cgatcgatag
     2221 ggcatagtcg aaaacccgaa tgactcgtgg gccgtagctt cctgctcggc ctgctcgacc
     2281 agatctcgca tgtattcctt gagcaatccg acaacatgtc cagagagcgc cattcccaac
     2341 tcctcacgcg ggcattatgc gtccatggag ggttcagagc aaccggcccg cctcgcttcc
     2401 tcacaggtaa cgcatttgac caaccggttg agggttttct atgatgcgcg gatggcgatg
     2461 ccttctgtcg agcgattcga aattgcgctg aatacgccgg ccggacgtct gacgacgacc
     2521 ctcgatgtcc cgaccggctt cgtcccgatt acggcgatcg tcccggtcgc gaggcgtctc
     2581 ggcgaagaag ccgtgcaact cgaagagcgg cacgcgaagg aaaccggcca agccatttca
     2641 tgccggatgg gctgtgccgc ctgttgccgg atgatggtgc cggtttcacc gccggaagcc
     2701 ttctcgcttc gagattatgt cgagcagctg ccggcagatc agcgtgaccg tatctccacc
     2761 cgcctggcgg aaaccaagaa cgtgcttcag aaacaaggat tgctgacccg gctccatgac
     2821 gtggccaacg cctcgaaagc tctcccggac gaagaactgg agccggtcaa ccgcgcctat
     2881 tacgcgttgc gcctcccttg tccgtttctt gaaaatgaaa tgtgttcgat ctacgaagcc
     2941 agacctgccg cctgccggga gctcctcgtg acctcgccgg cagaactctg ttccgacatc
     3001 gtgggaaatc ccgtgcgccc cttgccggtt ccgattcgca tcgggaccgt cctcggcctt
     3061 gtctgggctt cgctgacaca atccaccccc cgcctgattc cgcttccgct cgcgttggat
     3121 tgggcggacc ggcatcgaca ggaatccgcc caaacctgga caggatcggg tctcattgac
     3181 caaatgctcg acaagatgtg gcggttcctg agccaggagt tccacgcccg cggccagacc
     3241 cctcaagggt gattggtcat ccggttggtc tgtgactctg aaccattgaa cgaaggagtg
     3301 tgtccatgca gaagcctgtc atcgcctgtc tcgatctcga aggcgtgttg gttccggaaa
     3361 tctggatcaa cgtcgcgttg aaaaccggca tcgaagaact caagatcaca accagggaaa
     3421 tgccggacta cgacgcctta atgaagcgcc gcctggccat cctggatcag caccggctca
     3481 cgatcgccga cattcaggcc gtgattgata agatgggacc gctggagggc gcgccggcct
     3541 ttgtctcctg gctccgtgaa cggtgccagc tgattattct gtctgatacc ttctatcaat
     3601 ttgccctgcc cctcatgcgc cagctcggat tcccgaccct cttctgcaat cagctggaaa
     3661 tcgacggcag cggccgcatc gcgaattacc atatgcggat gcagaatcag aaaaaacatt
     3721 ccgtcgccgc gttcaagtcg ctcaactttt ttaccatcgc ggcaggtgat gcgtacaacg
     3781 acaccgccat gctgagcgaa gcagacgcag gcttcttctt ccgcccgccc gattacctcc
     3841 cgaaggaatt tccgcaattc cccgtgacgc aaacgtacaa ggagctgcag catcgattca
     3901 gccaagctgg caacctacgg ccatagtgcg atccagaggg atgttcaaaa aggcgacctt
     3961 ctcacccacc cgccccggcg cgctgaagca cgcccttcca caggctaggc cgcaccaagt
     4021 gaagccgagg aggtacatac caagctttgc ttgaaccgct cgcttcgatc atatgcgagc
     4081 ggataggtac tttgccccca gtgcgtttag tttggtacgt cgagggtctg aacgacggga
     4141 gaacgacgct ggacgtcttt ttcaacatcc cgtcgaaaac aagaagcccc gtgggggatc
     4201 tccccgcggg gctttctctt gattaggctc gaagcgggtc gcgccagacg cctaacgttt
     4261 cattcgctac tcgggaattg gggtttcgcc ttacgcctga cccctcaccc cttactcgtt
     4321 cctagaagtt tatccagagc tgggcgtatg cccactcttg atcgacgctg gtacccaggt
     4381 tttcctggat gtatccaccg gcaaacaggt gaccataggt ggcctggaaa gccagctttc
     4441 cgtccatgaa catatgggtc caggtgaagt cgatttcgtc accgatgtgc ttcttggtgt
     4501 tgccattctt ggagaaaaca tacactccct gcgccccgcg ataccagtta tctttggcgt
     4561 tcgcgagatt caggttggtg taccacactt cgatatggtc atccttggtg ggcctggctt
     4621 ggaagttgac cgaggggctc aacatgttct tccaggcttg gacatccatg tagcccatgt
     4681 ggatgtggtt tgtcgggaac aggttctcaa acgtattggc tgttttacaa ggggtggtgc
     4741 ttcccgccag cgtacagttc gcccgaccat caccagaggc ataatcgaag ttgaacgcca
     4801 aacgcggctt ccaggctgat tgataggcgg tgtagccgat ccagtttctg gtcgcccagg
     4861 cgctgatatg aaggttcttc tgattgcctt gggcagccaa tgcaccggca ctgttgtcgc
     4921 ccatctcgcc gaattgatag acggtttcgt tggtcgcatc gaagttaccc ttgcgcatct
     4981 cgatccggtt cccgacgttg tgacgggtct ggttcgcgtg tttcgccgtc ccgacgccct
     5041 ggccccggtt gtcgatcgtg ttgtaccggt tcgagtagag gatataatac ggttcgatca
     5101 ggaatcctgg caccgacttg atctggttgt agaaaatcaa catgtcggag tcagtattcg
     5161 cgctgccgtt gttgattgcg ctcgggccaa tgttcggtgc aaggctcccg accggcgatg
     5221 cctgtccgag atcagactca gaagtgcgga accaacccag gtagctatcc caggccttgg
     5281 tgctgtattg caacatcacg ccgtcatggg agaaacccgt attggcccag tcgaagtggc
     5341 cgaacaggga gtggtttccg aaaaccagat actgccgacc ggccttgacg ctgaagccct
     5401 gtatgccggc aaagttccgg atcaacatgt aggcagcacg cacacctaaa cgtccgcagt
     5461 tccctccacc gttgttcgtg gaacaaccat tgtggttcag tgcgtcgact tgtgctcccc
     5521 cggtaccatt ggccccgttg gcgccccaca ccgccgaatc gatgatttcc atgtagaaat
     5581 tcacatccgg cgagaggtcg tagccgatgc caaggcgtac ccactgttga acaaagaaat
     5641 cgttcgattt tcctttggtc ccgttcgctg agtttcctcc gaacgcatta caagcccccc
     5701 caccgacgct ggtactgccg aaacaaccct ggttgcgcat ttccggccgt acgcgcaagt
     5761 ccgcacgaag ccagaagttc ttgatgtcaa aattcttccc gatcttcgga tcgtaaagtt
     5821 cgtacgcctc cttttgtgga atcgcacgtg gaatggcggg gacgttggta atcttctccc
     5881 cctccggtag ttcgaacgcc gcttgagccg ggaccgtcac aaaggacatg cccgctgcca
     5941 tcacggccac gctgaagaag gtagccacac tggcccaccc tgttcgacct aggatgcttt
     6001 tcataacgtt cctcctgtga attggataac agccttcacc gttgtccgtt ctcggtgtcg
     6061 tactgccgac ttgacatcca actgcgccca cgttgacgaa ctcccgattc cttccaattg
     6121 agaggctgtc gtgcccacgc ctctcgactg ccgacgaaaa acccgcttcc caccctctgc
     6181 tacgtacgta ctctctcacc aaccgtttta cttgaccagc aatttctcat ttaccgagta
     6241 cgaccctctc acacttgcgc tcctgccgtc gatgaagagg cagtcacctc cttcccgaac
     6301 agtggtacag tgtgctgacg taccggccac cttcctcgtc tcgagcaacc gctaggcctg
     6361 gtttgccggt cctgatgaac cggcctgctt gtcgctcggc gtggtggcct gattgatttc
     6421 ttcttcagcc tccttcatgg acgcctggaa atcctgctcc accatcttga cttcctgctg
     6481 aatcatgttg agctccggct caaccgattt tctcaggtct tccgccgttt ccttaaagcc
     6541 cttgactgct ttcccaactt gccggccgac ttcaggcaac tgcttggggc cgaacaacag
     6601 gaacgcaatg accaggatga tcagtatttc gccggcaccc agtccgaaca tgtgcttcag
     6661 tgctgagtcc tgagtggtga ggactgagat tcggactcgt tttcctcatg cccaggcctc
     6721 agtcctccaa cctcggtctt acccagacta cccctgcttc gcctgctgcc cttgctgagt
     6781 ttgtgccgct gcttccggcg gcgtctgccc ctgggccgcc tggggctgca ccggcgccgc
     6841 cgcatcggca ggcggaggcg tcacatcgat ggcatcggct tcgttaacgc tcttcttaaa
     6901 tcctttaatc gctttaccga gcccctcacc caattgcgga atttttccgg cgccgaaaat
     6961 gatcaggaca ataatcagaa tcagcagcag ttccatccat ccgaatgagc cgaacattgt
     7021 gcaacgctcg ctttcttttc tgtcccgccc gtggagtacc gttcaattaa cgtcaccgga
     7081 tgcagtacga aaggcgggga tccttccaca aacgggggga aggctataga aatacaggag
     7141 attagtcaag tctaatttca cgtattaggc cattcggccc tatcgaatct tatgtggatc
     7201 agcggggtcg gatcgagaga tgaaaataat ctaccgggct gggtggtgcc gtaagggcga
     7261 tgagaaatga ttgagtatca tacccgacgt cttccagatc tttggaagac agcgtcagct
     7321 cttcttccgt gatataggcc accgtatcag gcactccgcc gggcttgagc tctcgaacca
     7381 aggcctggag catgtcgcgc tcttctgtcg gcatgtccgc tcggatcggg aactccatgc
     7441 ttctcgcgta ggggctctga taggtttcgt tcaatgcctt gatggtcttc aggatcagcc
     7501 gatcctgttc ccagggttcc atcttagaac cggcgagcgg atgcgtggcc aggagatcca
     7561 tgagcgttaa cacattcgtc ccgaggcttc gaccaatgaa ctcacgctgg ctttctattg
     7621 acaccgtgcc ggcctccagt tgcttgacga cggcttcggc aagggtgaag tttaccatga
     7681 agctctgctg gccgccgaat tggccggagc agggcttgtc aggatcgttc aataccttca
     7741 gatcggctgt gcctgcatcg aaactactga acccgaccgc atccggcgcc aagagacgtt
     7801 tggcctcctt gatggtggca tgagcgccga tattcaccgg caccaccacc tgctcgtcga
     7861 tccggcgcag aaattccgtg atggtcttgc gatagggaac ttctttccat tccaccgccc
     7921 gatattcccg ttcccacacc agatcgtcga aaaacggcgg gaaggtcttc agggtctcga
     7981 cgtctttggc ttcgaatgca cggacgaagg tggcccagtc ctgaatcttg gcatgaaggg
     8041 cttcgctcag gttcggcctc aggaattcct cctcgatctc gccggcctga cgcgacatga
     8101 gtttcgtcgg caaatcgttc cataactcat tgcagaaaat gcgatcaacc gtgccatccg
     8161 ccacccctgc cacctgatcg accactgagc agagcgtctc gactcgatcg cgatgagcgg
     8221 cgagtaccgg atgggctgta gccgcatcga gcatggtttg ctcccaatcg accagcacgt
     8281 aacggacacg gggatagatc agcccgtctc ggtccaacgt tttcaggtgg gtcagaaaac
     8341 aggcagcgag attaccattg ccgggcccga gttcgagaac cgtgaggggc gacgcagggg
     8401 atagggagat agacggggta tcggtggaac ctgattctgc actaccccgc tgacccgttg
     8461 cccctttgcc ccgttttttt tcacgtgtga tgactttttc gtaataatcg gtcgccagcg
     8521 cgtgagcgag ccggtgatcc gccgaggcga acgtttgata gtagcgacgg atctgatcgc
     8581 ctcggaggcg atagaagagt tgattgatat gcacttgcca ctgatcgacc ggcttatagt
     8641 cgccgatcag ctggggtaag gcatccgcat cctgcaacat acattcacct aggctcgagg
     8701 caaggggcta gaggcaagtg gcctgattcc ttgctcggat aagacttccg atcattttcc
     8761 ccagttggac acattcaact tcaacactgt cgtaggtgga ccgttccaca tagccgacat
     8821 cataggcgat ttggaatggt gtgcgtaatt cggttacaga tcctttcgcc atgtaaaaga
     8881 aacgaatggc ttcacggtcc gtgccgcgct catcaccttc cgcgaggttg ctcgcaacac
     8941 tgatggcgct gcgccgcatt tgatctctca atccgaaacc tttcctcagc tccgtacgat
     9001 cggatatatg atagatcttc accgccaaat cttttgcctt ttgccagacg agaaggtctc
     9061 cgaaaccctt cctgcccatt gcctcttgcc cttcacccct cgccctgcgc tcaaggagcg
     9121 cgaatcttag cagatggttg gaaacagccg caacgggctt tcacctcagc tttcagccgt
     9181 cagctatcgg cctgggacca gccttacaga cgactccttc tgcatttcct cacactgaat
     9241 gctgacggct gagggctgat gaatgtcagc tatttctgct ccttgacagc ccctattcat
     9301 cggtgtacga actgagccat gcgcctctcc gtcgttgtct cactcacgct atgctcttgt
     9361 cttctcggtg gcgcgttggt gcaggcagga gatctcccga tcaccaagaa tcctcccatc
     9421 cccgaattcc agaaccgcct gcaagatgcc aagccctatg actttgacgc gccgccccaa
     9481 ggaatgtttc gagccatcac gatggcggag ggcttcgacg aggagctggg atttcgtcgg
     9541 acccacgaga tcgttcccgt caaaccgaca gagcagtttc gtccagatgc cgcgggggtt
     9601 ttcatcgtct tccagctcca tcaacattat caggcatttc aagtgttcgg ccgttgcttt
     9661 cctgagtcgg tgccggggct cgatccgcgc acgatcgtcg gccaggatgc catgtacatc
     9721 gccctcgaag atgaatcagg ctatctgaaa ctcgcaccgc ctgaaggcgg gtggaaaccg
     9781 gggcgataca aggtggaaat tcacgcgggt gagcagatca acgagatgag ccttatgggc
     9841 acgatgcggt ttacggtgct tgcggaaaaa cagtagcgag acaggcatga aaggcgcggg
     9901 aactgctctc tgcgcgaagt cgctgccggg ccgcatagtt tgacccccct ccaggccttc
     9961 tgtagaatgc gcagatgatt tcttcatcct tctcaaatgc cgagcgtttc agttcatcat
    10021 gaccgatgca ccccgcccat gggcctccgt cgtcatcccg atcaaggatg aacgagacaa
    10081 cctcgctccg ctcaccgaac gcgtgctcaa ggtcctgggc gcacggaacg agtcgcgctc
    10141 ggcgccctat gaattgatct atatcgacga cggcagcacc gatggcagca gcgaactgct
    10201 ggacgaactg gcccggaact atccccccgt gcgcgtcctg cattttgacc gcaactacgg
    10261 gcaatcctcg gccttcgatg ccggattcaa acattccact ggcgcactcg tgatcacgat
    10321 ggacggcgac cttcagaatg atcccgtgga tatagagacc ttgcttcctc tgacacagag
    10381 ctacgatctt gtctgcggct ggcgcactac acgcaacgac aatctcgtgc gtaaactgtc
    10441 ttctcgtatc gccaatgcag tacggagcgc gataaccggg gaccgagtcc acgataccgg
    10501 ctgctcgttg aagattttcc ggcgggccgt cgtcgataaa ctccagttat tcaccggcat
    10561 gcaccggttc tttccggccc tggcactcat gcatgggttc accgtgaccg agatgccggt
    10621 gagtcactat ccccgggccc acgggatttc caaatacggc gtcggcaatc gactcttcaa
    10681 gggcttgtac gatctcatcg cagtgcggtg gatgcaacat cgtgtcttgc gataccagta
    10741 tcgacatccc tcgctgcatt cctgagtcca tccacctcga acctcgaacc tcacgactat
    10801 gacgatgacg gaaacaatct ggattgcaat cggatttctc gggcaggggc tctttttcgg
    10861 gcgttgggtc cttcagtgga tcgtgtccga acggaaagcg aagagtcagg ttccgctggc
    10921 attctggtac atgagcctga tcggcggact catcacgctg gcctatgcca tctatagaaa
    10981 ggatcccgtc ttcatcgcgg gtcagggggt cggtgctgtc gtctatatta gaaatctcgt
    11041 gctgattcga cgagcgggca cctcgccgac gacaaaccct gcacaatcgg ccgggaactc
    11101 ctgattcatt gcgcagacga cgctgattgc cttatgtcat catcctccat gccgacctca
    11161 aaatatgtcg tgaatctcgc agtcctcctg agcctcgcaa tcctgctcct cttcgtcgga
    11221 ttgggatcga tcgggctgac cgatcgtgac gagggccgca atgcggaggc cggacgcgag
    11281 atgctggaaa ccggcgactg gatgacgccg accttcaact acgaaccccg cttctacaag
    11341 ccggctttcc tgtattggct catgagcggc tcctacgcga ccttcggcgt ggacgaattc
    11401 gcagcacgat ttccttccgc cctctttggc gcagcactga ttctgctgca gtactggttt
    11461 ctctcccgat accgcaatgc gcagatcggc ctcttcggcg cactcatgtt gctgctgaat
    11521 atcgagatga tcgggctgag ccggatggcg ttgacggaca gtgtcctcgt gttctt
//
LOCUS       DATIFR010000181         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1267701, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000181 DATIFR010000000
VERSION     DATIFR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267701"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VLD60_10420"
     CDS             complement(<1..1009)
                     /locus_tag="VLD60_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSB45418.1"
                     /translation="MVTQLLNLIFGSKNDREIKALMPIVERINALESGLTPLPDQALA
                     DRTQEFKKRLTAGETLEDILPEAFAVCREMSRRKLNMRHFDVQLIGGMILHKGRIAEM
                     KTGEGKTLVATLPIYLNALEGKGAHLVTVNDYLAKRDAQWMGQLYHALGLSTGVIQHD
                     ASFIFDPTYEASDKRLQHLRPCTRPEAYRADITYGTNNEYGFDYLRDNLVVTDLNQCV
                     QRELNFSIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINAIIPQLKAEHDYTIEEK
                     TKTAALTEEGNVRVEKLLGVDNLYDPGNMDLVHHVVKALQAYALYKRDVDYVVKDGEV
                     II"
     gene            1194..1496
                     /locus_tag="VLD60_10425"
     CDS             1194..1496
                     /locus_tag="VLD60_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45419.1"
                     /translation="MLHGRMQRFLACVLVLCILAIGGFAAAQSLTHDVQHSHHQKATH
                     GTVLCSWMCAAGQAGEVSTVLMPLHVVPQEFAEQSSTASIQSNFSCSFSTRAPPVR"
BASE COUNT          316 a          451 c          504 g          412 t
ORIGIN      
        1 cgatgatcac ttcgccgtct ttgacgacgt agtccacgtc gcgtttgtac aacgcatagg
       61 cttggagtgc cttcaccacg tggtgcacga gatccatatt gcccggatcg tataagttgt
      121 cgacaccgag caatttttcg acgcggacgt taccctcttc ggtgagggcg gcggttttgg
      181 ttttttcttc gatcgtatag tcgtgctccg ccttcagctg gggaatgatc gcgttgatgc
      241 ggtagtagag gtcggtcgtc tggtccgtcg ggccggagat gatcaatggc gtccgcgctt
      301 cgtcgataag gatgctgtcg acttcgtcga cgatggagaa gttcaattcg cgctgcacgc
      361 actgattcaa gtcggtgacg acgaggttgt cgcggagata gtcgaagccg tattcgttgt
      421 ttgtgccgta ggtgatgtcg gcacgatagg cctccggtct ggtgcaaggt cgcaggtgct
      481 ggagccgctt gtcggatgct tcgtaggtcg gatcaaagat gaacgaggca tcgtgttgaa
      541 tgacccctgt ggagagccca agggcatgat agagctggcc catccactgg gcgtcgcgct
      601 tggcaagata gtcgttgacg gtgaccaggt gggcgccctt cccttcgagc gcgttcaggt
      661 aaatcggcag cgttgccacg agggtcttgc cctcgccggt tttcatttcc gcaatgcggc
      721 ccttgtgaag gatcatgccg ccgatcagct gtacatcgaa gtggcgcata ttcagcttgc
      781 gccgggacat ttcgcggcag acagcgaagg cttcgggcag gatgtcttcg agtgtttcgc
      841 cggccgtcag ccgtttcttg aactcctggg tcctgtcggc cagggcctgg tcgggaaggg
      901 gagtcaggcc ggactccagg gcgttgatgc gctcgacaat cggcatcaag gccttgattt
      961 cacggtcgtt cttgctgccg aagatcagat tcaaaagttg ggtgaccatg cgatgattat
     1021 ataaagggtg ggagttccga gcgcaagcgt taagacgaag ttgcccgagc agtataccgg
     1081 aaagcttttc tgaatcctat gaggaagata gccacaccgc gggccggctg atcttgactc
     1141 ctttgacccg gctccagtac catcgtgtcg ccggtcctga tagggcgttc ctcatgctac
     1201 acggtcggat gcaacgtttt ctcgcctgcg ttctcgtcct gtgtattctg gcgattggcg
     1261 gcttcgcggc cgcacagtcg ctgacgcacg acgtccaaca ttcacatcat cagaaagcca
     1321 cccacggcac cgtgctctgc tcctggatgt gtgcagcagg gcaagccggt gaagtctcca
     1381 ccgtgctgat gcctctccat gttgttcccc aagaattcgc cgaacaatcc tccactgcca
     1441 gcattcaaag caatttctcc tgcagttttt ccacccgcgc cccccctgtt cgttagcatc
     1501 gatcttattc gactctcgca agcggttatt ctctcggaat gagggagtct tgccgcggca
     1561 agcgacatgg cgtgtcgaat atcaaggttg accatctgtt gatggagagg ctgacgatga
     1621 aaacgttcct tagaaatacg agcaacatca ggctggttct cgcgcttgcg gtggggctgg
     1681 tcg
//
LOCUS       DATIFR010000182        12754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_503033, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000182 DATIFR010000000
VERSION     DATIFR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503033"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VLD60_10430"
     CDS             <1..1043
                     /locus_tag="VLD60_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB45420.1"
                     /translation="FEPPSPAINGTTVAFANLTVSAGGMDHDQLVVKWEEEPSARTLY
                     LKDPVLISAFRRAAPPELTHHFEAAATKVREARTHRRRMWGLALGSVGAVLLFLWLGS
                     DWLVELAVSRIPVEWEQKLGESAYRDFLAQQTVIKEGPAVAAVHEMTQRLIEKIPDNP
                     YKFEISVVKSDVVNAFALPGGYVVVFTGLMKKAESGEEVAGVLSHELNHVLQRHGLDR
                     MVKQLGLIAVVTIVLGDQQGLIGLMKQVGVELLTLKFGREQETEADITGLQLLQRAKI
                     DPNGMIRFFERLSEKDKERVEILSTHPMSEHRAARLRAEIAALPKQQPEPFEFDWKTV
                     QESLGVHEAVRP"
     gene            1040..1597
                     /locus_tag="VLD60_10435"
     CDS             1040..1597
                     /locus_tag="VLD60_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSB45421.1"
                     /translation="MKRLRSMRIGVIADTHGLYDPAIEDHFAEVAEILHAGDIGGQCV
                     IQRLRRIAPVIAVSGNVDNYEKSGFPRRTILRRGRTTIAVCHVLYENGQLTKDAREWL
                     DREQPDVCVFGHSHRPAIERYGATMLFNPGSAGPRRFSLPRGIGLLTVGRGKPKPLLV
                     RLPDRVEGTKKFTRKRTERTKGKPS"
     gene            1594..2172
                     /locus_tag="VLD60_10440"
     CDS             1594..2172
                     /locus_tag="VLD60_10440"
                     /inference="COORDINATES: protein motif:HMM:NF023238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HSB45422.1"
                     /translation="MNTRHLVRTALTGFVLVCFLTVTSACYGPFNLTRNVYHWNSNIK
                     GSGEVNEKWMKEIVFFGMIVIPVYMFSALLDAFIFNSIQFWGGDNPVKATDAGGDGAI
                     RVVRLGGITMTMIDGDRGTQVTYERNGIVERRAMIETSTAGYRLIDETGALLAEAEMG
                     QDKSVTLLDRDCQVVRRWTSDQLMALAGETQE"
     gene            2377..2889
                     /locus_tag="VLD60_10445"
     CDS             2377..2889
                     /locus_tag="VLD60_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HSB45423.1"
                     /translation="MMKKLVMLSVAVLFVGAQVGLAMAANPDTGPGCGLGKLAWGDYK
                     GQKEIAPQVLMATTNGTFGSNTFGISFGTSGCTNDGKIMSEHKTTMFAQLNFENLSQE
                     MAQGQGEHLASLATLMGVPAEHQAAFFAMTQERYTSLIKTGEASPVAMVKAINDAIAT
                     HPVLAQVSAQ"
     gene            2951..4858
                     /locus_tag="VLD60_10450"
     CDS             2951..4858
                     /locus_tag="VLD60_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HSB45424.1"
                     /translation="MLSPFLAAWIFVCSAGWVAAQAPPSNPYLEELLSHARALGLADE
                     REWQLLLHYRSSLFGGYESEQDDPGFFMSSRGKTDPQAELEATLTRFFSDELVGRSKQ
                     PAQCAFVARYHWLKERLAFDGAHLPAIACERFERWYADFDVQSITLIFPSAFMNNPAS
                     MFGHTLLRVDQKGQTEQTRILAYTINYAADVQPDAGLAYPIRGIFGFYKGYFSTIPYY
                     LKVQEYRDIENRDIWEYRLNFTPVQLRRLLMHAWELGNAYFDYFFFKENCSYHLLALL
                     EYANPSLHLTDQFTVWTVPADTVRLVAAQPGLVNEIAYRPSRSTIIRRKRESLSPEER
                     AQSARIAENAAELTAPAFAALSRKRQAFLLDVASDYLRYRIETTDSPPAGLKERHREI
                     LTARSELRIPSDPFSVVPFAKQPELGHKTSRAAIGGGWRNDDTFEEFTVRAGYHDLLD
                     PEVGYTPDAQIELASVSLRHYNRVDQTRIERATFANVLSLSPMDALFHAPSWKINMGM
                     NTIHFGDCQLCSNGVVNGGIGTAVESHWLKREVLFAFAEAEANYSRAYEENHRVGGGG
                     TVGMLADLTDRWKLMASGTYLRYALGDKSDDFRWFVGQRYTMSQNWALRLEYNHRDHD
                     NDVIFSVQAFF"
     gene            4878..5642
                     /locus_tag="VLD60_10455"
     CDS             4878..5642
                     /locus_tag="VLD60_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB45425.1"
                     /translation="MSVLDQLFIYHPDPWTDQDWKARSGLPLEEVWFQAADGARLFGW
                     YVETAADRPVLLWCHGNAGNVINRLDNLRYLYRQGLSVFLFDYRGYGRSQALRPSEEG
                     LYQDALGAYDYLTRTRMVRPERIVIFGRSLGAAVAAEVASQKPAAGLILESPFPSIEA
                     VARYHYGGLPVHWLLGAEFRLIDRLPQLSLPKLIIHGEKDDIIPIELGRMVFEAAKPP
                     KAFLPIPGADHNNTYQVGGAAYFERFADFCREAVRI"
     gene            5787..6746
                     /locus_tag="VLD60_10460"
     CDS             5787..6746
                     /locus_tag="VLD60_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HSB45426.1"
                     /translation="MTHSFLGWASGLLGLIAIVLTGCAEHVGSGTQPSLVGPSISKDG
                     GATAAMDKAPSVGGSSQPVAAATHVAVDTSPAQPAGPDSQPEEPFDPFAKADEAVGEE
                     YDPWESFNGKIFEFNRQLDRFVLKPVAKGYNFVAPDWVQVGVSNIFTNLRFAPRFLNN
                     VFQGKFKGAGIEMGRFLLNSTLGGAGLVDVATDMKITTPEEDLGQTIGSYGVGPGPYL
                     VLPLLPPFTVRDFVGFLGDIALNPINWLVAPIIEVNNVPSLIAHKNRMTATFVQSGSR
                     VGEIINERSRNLEKFQGVEEATLDLYTAVRNAYLQRRAKAVKD"
     gene            complement(6857..8047)
                     /locus_tag="VLD60_10465"
     CDS             complement(6857..8047)
                     /locus_tag="VLD60_10465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HSB45427.1"
                     /translation="MSYSSLKKIIDQQLAEIRSRGLYKSERQLLGPQGPDIRVAQGHV
                     LNLCANNYLGLANHPDIVRAATTGLQDHGYGMASVRFICGTQDLHKRLEQAISTFLGT
                     EDTILYSSCFDANGGLFEVLLDERDAVISDALNHASVIDGIRLCKAKRLRYAHSDMTD
                     LEAKLIDARECRLRLIATDGVFSMDGDLAKLDRIVELAERHDAAVMVDDSHATGVLGR
                     NGRGTPDHLGVADRVEIVTSTLGKTLGGATGGFTTGRAEIVELLRQRSRPYLFSNALP
                     PSVAAGALRALALVAQGDALRATLHENAALFRAQLTTLGFRLIPGEHPIIPIMLGAAS
                     LATAMADRLLKEGVYVVGFSYPVVPEGQARIRVQMSASHTHEQLEQAVVAFKNVGREL
                     GVIP"
     gene            complement(8137..9171)
                     /gene="tdh"
                     /locus_tag="VLD60_10470"
     CDS             complement(8137..9171)
                     /gene="tdh"
                     /locus_tag="VLD60_10470"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HSB45428.1"
                     /translation="MRALVKTSPQPGLTATTRPDPTPGPHDAVIRVKATSLCGTDAHI
                     YNWDPWAHSRVHPPRIIGHEMCGEVVEIGSAVSLVKVGDYVAAESHLTCGTCFQCRTG
                     QAHVCRNYRILGVDRDGSFAEYVALPESVLWETSQVVPPELASVQEPLGNAVDAALVD
                     DLTGHTVLITGCGPTGLFAAAVARTSGAATIIASDVSEYRLGLAKQVGVDHVLNVGTE
                     TPEAVAEAIREITAGEGVDASLEMSGNPGALHLAFSSVKNGGRVTLFGIPTGPITCDL
                     ANDIIFKGIRVHGITGRRLFGTWYRLAGLFKAGLNIKPVITHTFPMSDFAKGFDLIKS
                     GQCGKVVLFP"
     gene            9267..10256
                     /locus_tag="VLD60_10475"
     CDS             9267..10256
                     /locus_tag="VLD60_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSB45429.1"
                     /translation="MKALFHRSLMLLAGALCVSLPCWMSVAPVLSASSSGPAKRWAFF
                     ELDQSDSAGDLQAGKALTLAITLGGVTQGSVPVVALCESILFEPQTVTLEPDGESSRL
                     KATVTLAPMPQSRTSVHQKAARIQVTFARSRPDKLERFMKRIVYVTLDRQETPDDTTE
                     SAPLRPEEPLVGELIVDEVQPDVTPVSSAQLAEEDLMPLADPGQGKAYWQQVSYLVSR
                     SWARQVRGVRRGASGETVRVHFRLYPSGRAQLIEIEKGSGAREIDEAGIYAIVNAQPF
                     PPFPTELGDEAVDVHVRMRTGARAKSREAQSVGNPSSGKADAPPPASKNGGRP"
     gene            10296..10895
                     /locus_tag="VLD60_10480"
     CDS             10296..10895
                     /locus_tag="VLD60_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSB45430.1"
                     /translation="MLKRAVVVWLLGMASFWPAFVYAEPARWNVDPEHSTIEFRVAHM
                     VVSKTSGRFTDYTGFIDMDAEGGTVKAIEATIKTASVTTNHEKRDTHLRNPDFFDVEK
                     YPTMTYKLKSYKKTAEGYQAVGELTLRGVTKDITLAGTFNGVAKDPWGNTRAGFNAEG
                     RVNRKDFGMVWSKTLDGGGFVVGDEVYIKLDIECIKATP"
     gene            11006..12007
                     /locus_tag="VLD60_10485"
     CDS             11006..12007
                     /locus_tag="VLD60_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSB45431.1"
                     /translation="MNAMVLTGTKDVATHPLQRQDVPMPRPGPGEVLVRLTVCGICRT
                     DLHVIEGELDRPKLPLIPGHQAVGTISELGPGVEDRTIGERVGIAWLQGTCGQCDFCR
                     TGRENLCTAARFTGYQVDGGYAEYAVVPTNFAYPIPSVFADEEAAPLLCAGIIGYRAL
                     RLSGIQPGQRLGLYGFGASAHIAIQIARHWGCEVYVCSLKPEHQALARELGARWVGGA
                     SDRPPVPLQGSIIFAPAGELVPPALGALERGGTLALAGIHMSPIPVLNYDRDLFGERV
                     LRSVTANTRQDGVDLLREAAAIPIRPRTQRFRLEGANQALQALKAGTIKGAGVLAIQ"
     gene            12076..12669
                     /locus_tag="VLD60_10490"
     CDS             12076..12669
                     /locus_tag="VLD60_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45432.1"
                     /translation="MRRWILIGLMLGIWLLSGTSARALEFTADQITKIDGRTRKALIY
                     YRDQMWRIEHHSLGPVNVSIVRKDKQVVWLLLSRMKHFKTVPYDPEQDPWVTEKLEGE
                     ISRQEIGTEVREGHPTILYEVTTKQGERVDVYYQWIATDIHFPMKLAKKDGSWIVEYQ
                     HVKLRAVSDYLFNLPVNFQPLEDFDKDVPTESPQQGM"
BASE COUNT         2654 a         3516 c         3938 g         2646 t
ORIGIN      
        1 atttcgagcc tccctcgccc gccatcaatg ggaccacggt tgcgttcgcg aatctgacag
       61 tgtccgccgg aggcatggac catgaccaac ttgtggtgaa atgggaggag gaaccctcgg
      121 ccaggacact ctatctgaaa gatcccgttc tcatcagcgc atttcggcgt gccgcgccgc
      181 cggaactcac gcatcatttc gaagcggcgg caacgaaagt tcgggaggct cgcactcatc
      241 ggcgccggat gtggggcctt gcgctggggt cggtaggcgc ggtgctgctc tttctctggc
      301 tcggatccga ctggctggtg gagctggcag tgagtcggat accagtcgaa tgggaacaga
      361 agctcggcga atcggcgtat cgagattttc tcgcgcaaca gacggtgatc aaagaaggcc
      421 cggcggtcgc ggcggtgcat gagatgacgc aacggctcat agagaagatc cccgacaatc
      481 cctacaagtt tgaaatttct gtggtgaaga gcgatgtcgt caacgcgttc gccttgccgg
      541 ggggctacgt cgtcgtcttc accggtctga tgaagaaagc ggagagcgga gaggaagtgg
      601 ccggcgtgct cagccatgaa ctgaatcacg tcctgcagcg gcacggtctc gatcgaatgg
      661 tgaaacaact cggcctgatc gcggttgtga ccatcgtgct cggagatcaa cagggcttga
      721 ttggcctcat gaagcaagtc ggagtggagc tcttgaccct gaaattcggt cgcgagcagg
      781 agacggaagc cgacatcaca ggcctgcagt tgctccagcg tgcgaagatc gatcccaatg
      841 gcatgatccg atttttcgag cgcctatcgg aaaaggacaa ggagcgcgtc gagatcctgt
      901 cgactcatcc gatgagcgag caccgtgctg cgcgccttcg agcggaaatc gcggccttgc
      961 cgaaacagca gcctgagccg ttcgagttcg attggaagac cgtccaggag tcgctggggg
     1021 tgcacgaggc ggtgcgacca tgaaacggtt acgatccatg cgcatcggtg tcatcgccga
     1081 cacacacgga ctgtatgatc cggccattga agatcatttt gccgaggtgg cagagatcct
     1141 gcatgccggt gacattgggg gacaatgcgt cattcaaagg ttacgacgga tcgcaccggt
     1201 gattgctgtc tcgggaaatg ttgataatta tgaaaaaagc ggattcccca ggcggactat
     1261 ccttcgccga ggcagaacaa ccattgccgt ctgccacgtg ttgtacgaga acggacaatt
     1321 gacgaaggac gcacgggagt ggctggatcg ggagcaaccg gatgtctgcg tctttggcca
     1381 cagccacagg ccggcgattg aacgatacgg tgcgaccatg ttgtttaatc ccggctccgc
     1441 aggtcccagg cggttctcgt tgccgcgtgg aattggactc ttgacggtcg gccgaggaaa
     1501 gcccaagccc ttgttggtcc gactgcctga tcgggtagaa ggaacaaaga aattcacaag
     1561 gaaacgaact gaacgaacga aaggaaaacc atcatgaaca caaggcatct ggtgcgcacc
     1621 gcgctgaccg gcttcgtgct cgtctgcttt ctgactgtca caagcgcctg ttatggaccg
     1681 ttcaacctga caaggaacgt gtatcactgg aacagcaata tcaaaggcag tggtgaggtg
     1741 aatgaaaagt ggatgaagga aattgtattc tttgggatga tcgtgatccc tgtgtacatg
     1801 ttttctgcgt tactcgacgc gttcatcttc aactctattc agttctgggg cggcgacaac
     1861 cccgtcaaag ccaccgacgc gggaggtgat ggagcgatcc gcgtcgtccg gctgggcggg
     1921 atcaccatga cgatgatcga cggtgatcga ggcacgcagg tgacctacga gcggaacgga
     1981 atcgtcgaac gacgcgccat gatcgaaacg agtacggcgg gctatcgtct gatcgatgag
     2041 accggagccc tgctcgcgga ggcggaaatg ggacaggaca aaagcgtcac gttgctcgat
     2101 cgcgattgcc aggtggtgag acgatggaca tccgatcaac tgatggcgct ggcgggagag
     2161 actcaggagt aagaacaacc tcgatagaag caaaccgcct gcacggccca gagcggggtg
     2221 aacagttgcg catctgttcg cgtcggcctt gctctcagga tgaacgggtt tcgagttttt
     2281 tttctggcaa tccgaataag gttgagtata atcggtgagt acttgataca tatgtgccgg
     2341 tgcgtttaac cctattcctt tgtaggagga ggtcatatga tgaagaagct ggtgatgctg
     2401 tcggtagcgg tgttgttcgt gggagcccaa gtcggtctcg cgatggcggc gaatccggat
     2461 acggggcctg ggtgtggtct cggcaagctg gcctggggtg actacaaagg gcagaaggaa
     2521 atcgctccgc aagtgctgat ggccaccacc aacggtacct ttggtagcaa cacgttcggt
     2581 atcagcttcg gaacttcggg ttgtacgaac gatgggaaga tcatgtccga acacaaaacg
     2641 acgatgtttg ctcagctcaa cttcgagaac ctgtctcagg aaatggcgca gggccagggt
     2701 gagcatctcg catcactcgc caccttgatg ggtgtaccgg ctgagcacca ggcggcgttc
     2761 tttgccatga ctcaggagcg ttacacctcc ctgatcaaga caggtgaagc ctcaccggtt
     2821 gcgatggtca aagcgatcaa cgacgccatt gccacgcatc cagtccttgc ccaggtttct
     2881 gctcagtaag tcgatccgta gggctctggc ctatgcaggc cagagccctt tctattttca
     2941 cgctcatatc ttgctgtctc cattcctcgc tgcgtggata ttcgtctgca gcgccggctg
     3001 ggtcgccgcc caggcgccgc cgtccaatcc ctatctcgaa gaactccttt cgcatgcgcg
     3061 agccctcggc ctggctgacg aacgggaatg gcagttgctg ttgcattatc ggtcgagtct
     3121 gtttggaggc tatgagagcg agcaggatga tccgggattt ttcatgtcct cacgcgggaa
     3181 gaccgatccg caggctgagt tggaagcgac actgacccgg ttcttttccg acgagctggt
     3241 cgggcggtcg aagcagccag cgcaatgcgc ctttgtggcg cgctatcact ggctgaaaga
     3301 gcggctagcc ttcgatggtg cgcatctgcc ggcaatcgcg tgcgaacgat tcgaacgatg
     3361 gtatgccgat ttcgatgtcc agtccatcac gttgattttt ccgtccgcgt ttatgaacaa
     3421 ccccgcctct atgttcggac acacgttgct gcgggttgat cagaaaggcc agaccgagca
     3481 gacgcgcatt ctggcgtata ccatcaatta tgcggccgac gtgcagccgg atgccgggct
     3541 tgcctatccg attcgcggca tcttcggctt ctacaaaggc tacttctcca cgattccgta
     3601 ctacctcaaa gttcaggaat atcgagacat cgagaaccgc gacatctggg aatatcggct
     3661 caatttcacg ccggtccaac tgcggcggtt gctgatgcat gcatgggaac tcggcaatgc
     3721 ctacttcgat tatttctttt tcaaagagaa ttgctcctac catctcctgg cattgctgga
     3781 atatgccaat ccctccctgc acttgaccga tcagttcacg gtctggaccg tgccagccga
     3841 tacggtgcgg ctcgttgccg cccagccagg gctggtcaac gagattgcct accgaccctc
     3901 gcgcagcacc atcatccgtc ggaagcggga atcattgtca ccggaggaga gggcccagtc
     3961 tgctcgaatt gcggagaatg ccgcagagct cactgctccg gcctttgcgg cattaagtcg
     4021 aaagcgacag gcatttctgc tcgatgtggc gtcggactat ctacgctatc gaatcgagac
     4081 caccgattca cctcccgctg gcctcaaaga acgccatcga gagatcttga ccgcacggag
     4141 cgagctgcgc attccctccg atcccttttc ggtcgttcct ttcgccaaac aaccggaact
     4201 gggccacaaa acttcacggg ccgctatagg gggcggctgg cggaacgacg atacgtttga
     4261 agagttcacc gtgcgcgcgg gctatcacga cctgctcgat ccggaagtcg ggtacactcc
     4321 ggacgcccag atcgagctag cctcggttag cctgcgccac tacaaccgcg tcgatcagac
     4381 gaggattgag cgggcgacct ttgccaacgt gctgtcgctc tcaccgatgg atgcgttgtt
     4441 tcatgcaccg tcgtggaaga tcaatatggg aatgaacacg atccatttcg gcgactgtca
     4501 gctttgcagc aacggggtgg tcaacggcgg catcggaaca gcggtcgaat cccactggtt
     4561 aaaacgggaa gtgttgttcg ccttcgccga agcggaggct aattacagcc gggcctacga
     4621 ggaaaatcat cgcgtcggcg ggggaggtac cgtcgggatg ttggccgatc tcaccgaccg
     4681 ctggaaactc atggcgtccg gaacgtattt gcgctatgcc ctgggagaca agtcggacga
     4741 cttccgttgg ttcgtgggcc agcgctacac gatgagccag aactgggcgc ttcgcctcga
     4801 gtacaatcac cgtgaccacg ataacgacgt catcttttca gtgcaggcgt ttttctgagt
     4861 tcagtggggg atctcatgtg agcgtccttg atcaactctt catttatcat ccagatccct
     4921 ggaccgacca ggattggaaa gctcggagcg gcttgccgct ggaagaggtc tggtttcagg
     4981 ccgccgatgg cgcgcggttg ttcgggtggt atgtcgaaac tgcggcggat cgcccggtgc
     5041 ttctctggtg ccacgggaat gcaggcaacg tcatcaatcg gctcgacaac cttcgctatc
     5101 tctatcggca ggggctgtcc gtgttcctat tcgactaccg agggtacggg agaagtcagg
     5161 cgcttcgccc cagcgaggag ggtctgtatc aagacgcgct cggggcttat gactacctga
     5221 cgcgaacgcg gatggtcagg cctgagcgga tcgtgatttt cgggcgttca ctcggagcgg
     5281 cggtcgctgc tgaggttgcg tcgcaaaagc ctgccgcggg actcattctc gaatcaccct
     5341 ttccgtcgat cgaagccgtg gctaggtatc actatggcgg cttgccggtt cattggctgc
     5401 tgggcgcgga gtttcgactg atcgaccgcc tgccccagct ttcgttaccg aaactcatca
     5461 tccacggtga gaaggacgac atcattccaa tcgagctggg tcgcatggtg tttgaggcgg
     5521 ccaagcctcc caaagccttc cttccgattc ctggcgccga ccacaacaat acctaccagg
     5581 ttggaggggc ggcctatttc gagcgatttg ccgatttttg ccgggaggcg gtccgcatct
     5641 agctgcggca aggctaccgg cagccctccg taggtaagat tgtcttggcg attggcctga
     5701 gttttggtac agtgcgcctc gcgcgttccc ctggaacctg ctgcaccgtc cgacaggagg
     5761 ttctgagcgc atgaatggga gggccggtga cacactcgtt ccttggctgg gcatcagggc
     5821 tcctcggtct gatcgcaatt gtgctgaccg gttgtgccga gcacgtcggt tcgggaacgc
     5881 agccatcctt ggttggtccg agcatatcga aggatggagg cgcaaccgcc gctatggaca
     5941 aggccccgag cgttgggggc tcgtcccaac cggttgctgc tgcgacacac gtcgcagttg
     6001 atacttctcc tgcacagccg gcgggaccgg acagccagcc tgaagagccg ttcgatcctt
     6061 tcgcaaaggc tgatgaggcg gttggcgagg agtacgatcc gtgggaatcg ttcaacggga
     6121 aaatctttga attcaaccgt caactggatc gcttcgtgct caagccggtc gcgaaaggct
     6181 acaacttcgt cgcgcctgat tgggtacagg tcggggtcag caatatcttt accaacctgc
     6241 gattcgcgcc gcgcttcctc aataatgttt ttcaggggaa gttcaaaggg gccggaatcg
     6301 agatggggcg gtttctgttg aacagcacgc ttggcggtgc cgggctcgtc gacgtcgcca
     6361 cggacatgaa gatcacgacg cccgaagaag acctgggcca aacgattggg agttatggcg
     6421 tcggacccgg cccctatctg gtgttgcccc tgctaccgcc ctttaccgtg cgggattttg
     6481 tcggctttct tggcgacatc gcgctcaatc cgatcaattg gctggttgcg ccgatcattg
     6541 aagtcaacaa cgtgccatcc ctgatcgctc acaagaaccg gatgacggcg acatttgtgc
     6601 agagcggatc ccgcgtgggc gagatcatca acgaacggtc ccgcaatctc gaaaaattcc
     6661 aaggagtcga agaggccaca ctggatctct ataccgccgt ccgcaacgcc tacctgcaaa
     6721 gacgcgccaa ggcggtcaag gactagaaac gcgagctgtg aggcgtaagg ggtgaggggg
     6781 taggagtgtc ccatttggag gcggattctc aagggagaat aatccgtctt gcgcgtcacg
     6841 tttcacgcct gacgtctcac gggataacgc ccaattccct gccgacgttc ttgaaggcga
     6901 ccacggcttg ttcgagctgc tcgtgcgtgt ggctggctga catctggacg cggattctcg
     6961 cctgaccttc cggtacgacc gggtagctga acccaaccac atacacgcct tccttcaaga
     7021 gccggtcggc catcgcggtg gcgagggaag cggcacccag catgatcgga atgatcgggt
     7081 gttcgccggg gatcaggcgg aaccccaggg tggtcagctg ggcgcggaac aaggcggcgt
     7141 tctcatgcag cgtcgccctc agagcatcac cttgtgcgac gagcgcaagg gcccggagcg
     7201 ctccggctgc aacagacggt gggagcgcat tcgaaaacaa atagggtcgc gatcgctggc
     7261 gaaggagttc aacgatttcc gctcgccccg ttgtaaatcc cccggtggcc ccgccgagcg
     7321 ttttgcccag ggtgctggtg acaatctcga cgcgatcggc cacgccgaga tggtcgggag
     7381 ttccccggcc gttgcgtccc agaacgccgg tggcatgact gtcatccacc atgaccgccg
     7441 catcgtggcg ttctgccagc tcgacgatgc ggtccagttt ggcgaggtct ccgtccatgg
     7501 agaagacgcc gtccgtcgcg atgagacgca agcggcactc ccgcgcgtcg atgagtttcg
     7561 cttccaggtc cgtcatatct gagtgagcgt atcgaagtcg cttggctttg cagagcctga
     7621 taccgtcgat gacgctggcg tggttgagtg catcgctgat gacggcgtct cgttcgtcca
     7681 gcaagacttc gaagagccct ccgtttgcat cgaagcaaga gctgtagagg atcgtgtcct
     7741 cggtgcctaa gaacgtgctg atggcctgtt cgagccgttt gtgcagatcc tgagtgccgc
     7801 aaatgaatct gacggaggcc atgccgtatc catggtcctg caatcccgtt gttgccgcgc
     7861 gcacaatgtc cggatgattg gccagcccca gatagttgtt ggcgcaaagg ttcaagacat
     7921 ggccctgtgc aacgcggata tccggacctt gcggacccag cagctgccgc tcggatttgt
     7981 acaggcctcg cgagcggatc tcggcaagct gttggtcgat gattttcttg agtgaggagt
     8041 aggacattct taattcgtga ggcgtgaaac gtaaggcgtg agagcgtaaa tatgaaccta
     8101 aagtttccta tcgcctgacg cctaactcct cacacctcac gggaataaca ccactttccc
     8161 gcattggccg gatttgatca agtcgaaccc cttggcgaag tcgctcatgg gaaacgtatg
     8221 ggtgatcacc ggcttgatat tgagcccggc cttgaacagg ccggccaacc gataccaggt
     8281 gccgaacagc cggcggccgg tgatgccgtg gactcgaatg cctttaaaaa tgatgtcgtt
     8341 cgccaagtcg caggtgatcg ggccggttgg gatgccgaac agcgtgacac gcccgccgtt
     8401 cttgacggag ctgaacgcaa gatgcagcgc gccaggattg ccggacatct caagtgacgc
     8461 atcgacacct tcgccggcag tgatctcgcg aatcgcttcg gcaaccgcct ccggagtttc
     8521 ggtgccgaca ttcaagacgt gatcgacgcc cacctgcttg gctagcccga ggcgatactc
     8581 gctgacatcg gaggcgatga tggtcgctgc tccggaggtg cgtgccaccg ccgcagcgaa
     8641 caggccggtc ggtccgcagc cggtgatcag caccgtatgg cctgtgagat cgtcgaccaa
     8701 tgccgcatcg acggcattcc ccagtggttc ttgcacagat gcgagttccg gcggaacaac
     8761 ctgcgaggtt tcccacagga cgctttcggg cagggcgaca tattccgcaa acgaaccatc
     8821 gcggtcgaca ccgagaatgc gatagttccg gcagacgtgc gcctgtccgg tgcgacattg
     8881 aaaacaggtt ccgcatgtca ggtgggattc ggcggcgaca tagtcgccca ctttgacgag
     8941 cgaaaccgcg gagccgattt ccacgacctc gccgcacatt tcatgtccga tgattcgagg
     9001 cggatgaacg cggctatggg cccaagggtc ccagttatag atgtgagcgt cggtgccgca
     9061 aagcgacgtg gctttcaccc ggatgacggc atcgtggggt cccggtgtgg gatcaggccg
     9121 cgtggtggcg gtgagtccag gctgcggtga ggttttcacg agcgcgcgca tcggctcatt
     9181 ctataacaac cggaggatct tgcctaatta ttgtggaaga aaaaacgggt tgcgcgacag
     9241 tctgcggcct aggtaggatg ccgctcatga aggctctgtt tcatcgaagc ctgatgctcc
     9301 tggcaggggc gctctgtgtg agcctgccct gctggatgtc ggtcgcccct gtcctttccg
     9361 cctcctcttc cggtccggcc aagcgctggg cgttctttga actcgaccaa tccgattcag
     9421 cgggagatct tcaagccgga aaagctctga cacttgccat taccctcggc ggagtcacac
     9481 aggggtcagt tcccgtcgtg gccctctgcg aatcgattct ctttgagccc cagacggtca
     9541 cgctcgagcc agacggagaa tcgtccagat tgaaagccac ggtaacactc gcaccgatgc
     9601 cccagagccg aacgtcggtg catcaaaaag cggcccgcat tcaagttacg tttgcccggt
     9661 cccgaccgga taagctcgag cgattcatga agcgaatcgt atatgtcacc ttggatcgcc
     9721 aggagacccc cgacgatacc accgagtctg cgcccttgcg tcccgaagaa cctctcgtcg
     9781 gcgagttgat cgtcgacgaa gtgcagccgg atgtcacgcc tgtgtcgtca gcccaattgg
     9841 ccgaggagga tctgatgccg ctggccgatc ccggacaggg aaaggcgtac tggcagcaag
     9901 tgagttactt ggtcagccgc agctgggcca ggcaagtccg cggggtgcgg cgtggagcca
     9961 gcggcgaaac cgtgcgggtc catttcaggc tctaccccag cgggcgggcc cagttgattg
    10021 aaattgagaa agggtccggg gctcgtgaaa tcgacgaagc cggcatctat gcgatcgtga
    10081 acgcgcaacc ctttccgccg ttcccgactg aattggggga cgaggcggtt gacgtgcatg
    10141 tgcgcatgcg gaccggggcg cgagcaaaat cccgcgaagc gcagtccgtt gggaatcctt
    10201 cgtcgggcaa ggcggatgcg ccgcctccgg cctcgaagaa tggcggcagg ccataattcg
    10261 agctgctgtg ttctcaatca acaggagaat tgttaatgct gaagcgcgca gtggtggtat
    10321 ggcttctcgg gatggcctct ttctggccgg cgttcgtcta tgccgaaccg gcgaggtgga
    10381 acgtggatcc tgagcattcg acgatcgaat ttcgtgtcgc acacatggta gtgtcgaaaa
    10441 cgagcggacg gtttacggac tataccggct ttatcgacat ggatgcggag ggcggcaccg
    10501 tcaaagccat tgaagcgacc atcaagaccg cgtcggtgac tacaaatcac gagaagcgcg
    10561 atacccactt gcggaatccg gacttctttg acgtcgagaa gtatccaacg atgacgtaca
    10621 aattgaagag ctacaagaaa acggcggagg ggtatcaggc cgtcggggaa ctgacgcttc
    10681 gcggtgtgac caaagacatc acactcgcgg gaacgtttaa cggggtggcc aaggatcctt
    10741 gggggaatac gagagccgga ttcaacgcgg aaggcagggt gaatcgaaag gatttcggca
    10801 tggtatggag caagaccttg gacggaggcg ggttcgttgt cggcgacgag gtctatatca
    10861 agctcgacat tgagtgcatc aaagccacac cctaacaccg gcagctttat gaaaccggcc
    10921 tgcgagactg cggttgccgg ttcttgaagg tcgttctgac aggcagacgc cagccctgct
    10981 tcgaaccaac cacgagtcat ccactatgaa cgcaatggtg ctcaccggca cgaaggacgt
    11041 ggcaactcat cccttgcagc gtcaggatgt tccgatgcct cggccggggc ctggcgaagt
    11101 cttggtccgc ctcactgtgt gcggaatctg caggaccgat ttgcacgtca ttgaaggtga
    11161 gttggaccga cccaagctgc cgttgattcc cggtcatcaa gccgtcggga cgatcagtga
    11221 acttgggccg ggcgtcgaag acagaacaat cggggaacga gtgggtattg cttggctgca
    11281 gggcacctgc gggcaatgtg atttttgccg caccggccgg gagaacttat gtacggctgc
    11341 acgcttcacc ggctatcagg ttgatggagg ttatgcggag tacgcggttg tgccaaccaa
    11401 ctttgcctat ccgattccct ccgtctttgc cgatgaagaa gcagcaccgc tgctctgcgc
    11461 cggcatcatc ggctatcggg cgttgcgcct gagtggtatt cagcctgggc aacggctcgg
    11521 tctgtacgga ttcggcgcct ccgcccacat cgcgatccag attgctcggc attggggctg
    11581 cgaggtctac gtctgttcgc ttaagccgga gcaccaggcg ttggcccgtg agttaggggc
    11641 ccggtgggtt ggtggcgcat cggatcgtcc gccggttccg ctgcaaggct ccatcatatt
    11701 tgcgccggcc ggcgaactag tcccaccggc gctgggcgcg cttgagcgag gggggacgct
    11761 ggctctggca gggattcaca tgagtcccat tcctgtcctc aactatgatc gcgacctatt
    11821 cggcgaacgg gtcctgcgaa gcgtgacggc gaatacgaga caagacgggg tcgatcttct
    11881 tcgagaggcg gcggcgatcc cgatcagacc tcgcacacaa cggtttcgat tggagggcgc
    11941 caatcaggcc ttgcaggcgc tcaaggctgg cacgatcaag ggggcggggg tgttggcgat
    12001 tcagtaacac ggacatgatg ccgtgcccgt cagcgaagtg gaaatcacgt ccccgcccta
    12061 tgttaggatt caacgatgcg acgatggata ctgatcggct tgatgcttgg aatatggctg
    12121 ctgtctggga cttccgcccg ggcgctggaa tttacggcag atcaaattac caagatcgac
    12181 gggcggacgc gaaaagcatt gatctactat cgcgaccaga tgtggcgcat cgagcaccat
    12241 tcgctggggc cggtcaatgt cagcatcgtc cgaaaggaca agcaggtcgt gtggctgctg
    12301 ctttcgcgga tgaaacactt caaaacagtt ccatacgacc cggaacaaga tccgtgggtg
    12361 acggaaaagc tggagggaga aatctcccgg caagagatcg gcacggaggt gcgcgaagga
    12421 catccgacca ttctgtacga agtcacgacg aagcagggcg agcgggtgga tgtgtattac
    12481 caatggatcg cgacggatat tcattttccg atgaagctgg cgaagaaaga cggcagctgg
    12541 attgtcgaat atcagcacgt gaagctgcgc gccgtgtcgg actatctgtt caatctgccc
    12601 gtcaattttc agccgttgga agatttcgat aaggacgtac cgacagagtc cccgcaacag
    12661 gggatgtaac tgacaaggag gagtgattgt gcagaggaga ggatgtgtca ttggcctgtt
    12721 catcgcggga gtgctggggg ttgggagcct gggg
//
LOCUS       DATIFR010000183         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2611710, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000183 DATIFR010000000
VERSION     DATIFR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611710"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLD60_10495"
     CDS             <1..216
                     /locus_tag="VLD60_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45433.1"
                     /translation="WSQLKDKSCRQPVKTFYGTPPMHQLFERSTWHSAQHARQLMAVL
                     ERFDIEPQGRLTSEDLAGLPLPEGLWE"
     gene            complement(542..1780)
                     /gene="ltrA"
                     /locus_tag="VLD60_10500"
     CDS             complement(542..1780)
                     /gene="ltrA"
                     /locus_tag="VLD60_10500"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HSB45434.1"
                     /translation="MWNERMLAALENGVKGGKWFSLIDKVYRPQTLRRAWQKVRANAG
                     AAGVDGVSVKRFEAGAEKYLGELEEELKAGKYQAQPVKRVEIAKGNGKKRPLGIPVVK
                     DRIVQTALKLVIEPIFEREFEESSYGFRPKCSAKDALREVDRLLKEGYTCVVDADFDN
                     CFETIPREPLMREVEQRISDGGVLGLIRGFLQQDIIHEMKRWTPLGGTPQGAVISPLL
                     ANIYLHRLDRYLKEKGHRMVRYADDFVVLCRSAEQAQAALAEVRAWVEQNGLKLNADK
                     THVGDCREAGEGFEFLGYRFEAGWRWVRKKSLKALREQIRKKTKRTRGESLAEIIEDL
                     NPMLRGWFGYFKHAHGRTFGWIDGMVRRRLRAVLRKQEKRPGMGLCRADHQRWPNAFF
                     AARGLFTLEAAWEKLASQSR"
     gene            2484..>2991
                     /locus_tag="VLD60_10505"
     CDS             2484..>2991
                     /locus_tag="VLD60_10505"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB45435.1"
                     /translation="MSLQLTIACGDYDRTHPLIDGSVKPEGIELNWLVLPHLEIWTRM
                     LNYYDFDASEISLSSYIIARSIGKPLTAIPVFPARAFRHSYIFINTKSGIREPKDLIG
                     KRVGLAEFQQTATVWIRGTLQHEYGVNLDDIEWFTWVPHSRMEMELPKRYKVTHLTAD
                     KKPDQMLFN"
BASE COUNT          570 a          989 c          730 g          702 t
ORIGIN      
        1 tggagccaac tcaaggacaa atcctgccgg caacctgtga aaactttcta tggcacgccg
       61 ccgatgcatc agcttttcga gcgctccacc tggcactccg cgcagcacgc gcgccagctg
      121 atggccgtgc tcgagcgttt cgatatcgag ccgcaggggc gactcacgtc ggaagatctc
      181 gccggtctac ctctgccgga aggcctttgg gaatgaccaa gagataattt gccgctatca
      241 cctcgaattc gtttaccacg aagatcccag cgtggcagag ccgcaacata ttgtttaagg
      301 agagttttca ccacgtaggc acgaaggagc cgaagggttc ggacaattta atttccgaac
      361 ttcgtgctcg tagcctgtgg cgagcgtagt cgaaccatgt cctccttcga aaatacctgt
      421 gcggggatga gaacgaagcc gtcccgggtt ccgatagggg tcggaaacgg ctttcgccgt
      481 ccccgccctc cgaaccgtgc gtgcggttct cccgcacacg gctctccagt cggtagtttc
      541 ctcatcggga ttggctcgct aacttctccc aggctgcctc taaggtgaac agtccccggg
      601 ctgcgaagaa ggcattgggc cagcgctgat gatcggctcg acaaagtccc attcccgggc
      661 gtttctcctg ctttcgtaga accgcacgca gccgtcggcg caccattcca tcgatccatc
      721 caaacgtcct tccatgagcg tgcttgaagt agccaaacca cccgcgcagc atcgggttaa
      781 gatcctcaat gatctcggcc agactctcgc cgcgcgtccg tttggttttc ttacggatct
      841 gctcgcgcaa ggccttgaga cttttcttcc tcacccagcg ccaccccgcc tcgaagcgat
      901 acccgagaaa ctcaaaacct tctcccgctt cccgacaatc ccccacatgg gtcttgtccg
      961 cgttaagctt gagtccgttc tgttctaccc aggctcttac ctcggcgagc gccgcttggg
     1021 cctgctccgc acttcggcac aacaccacaa agtcatcggc gtagcgcacc atccgatggc
     1081 ctttctcttt cagatagcga tccaatcgat gtaaatagat gttcgccaac agcgggctga
     1141 tcaccgctcc ttgcggcgtc ccgcccagcg gcgtccatcg cttcatctcg tggataatat
     1201 cctgttggag aaaaccccgg atcaacccca acacccctcc atcgctgatc cgttgttcca
     1261 cttctctcat gagcggctct cgcggtatcg tctcaaagca attgtcaaag tcggcatcca
     1321 ccacacacgt gtagccctct ttgagcaatc tatcgacttc tcgcagcgcg tctttcgcgc
     1381 tgcacttggg tcgaaagccg taactcgatt cctcaaactc tctctcaaag atcggttcga
     1441 ttaccagttt tagcgccgtc tgcacgatgc ggtctttgac caccggtatc cccaacgggc
     1501 gcttcttgcc attccctttg gcgatctcca ctcgcttgac cggctgcgcc tggtacttcc
     1561 ccgccttgag ttcctcctcg agctctccga gatacttctc ggcccccgct tcgaaccgct
     1621 tcacgctcac tccatcgact ccagccgccc cggcattggc tctgactttc tgccaggccc
     1681 tcctaagcgt ctgcggccga tacactttgt cgatcaggct gaaccatttc cctcctttga
     1741 cgccgttctc cagcgccgcc aacatgcgct cgttccacac cccggcttcc acccatgacc
     1801 aatctcggcc tcgggtctct gcgccttgtc tagccctact ggacactcgc ggcgcctggc
     1861 ttttgccttg ccgttcggtg aaactcacgc ctctaccttc ctgcctccct tccctcagcg
     1921 ccgttttgct tccggcacct ctcgcaggtt ttttttttcc tgctgcggta ctatgaaggc
     1981 tctgactcct gcaagcctca ccgtccttgc aggtctcccc gcttactgcg ctccaccttc
     2041 gggacgttcc gtctccaacc acgtgggccg cttcgacatc gcttaccacc acgccagcgt
     2101 gccgaatgat tttcagactt cgccacgtat gagcaggctc gtcgcggctc cccgccaaat
     2161 cgagttcgct tttctacgga ccgccctttc gtctccagtt gctctccaca ccgcctcggg
     2221 ccagtgcagt tactttcgac ttcggagttg tggcatactc cggtcacgga ctttcaccat
     2281 gctgatgtag cgccctcacg ggcgcactca tacccgcgaa agcgggtatc caggctaatt
     2341 cggcgcaaca atcctggatt cccgcgtgcg cgggaatgac gaatcggagg agcaccgttt
     2401 ggagagaact cccgctattt ttcatactct gtgggcgagc gtaagctcat gaatcacttc
     2461 gtggtgaata aaatggagtc atcatgtcac tacaactaac cattgcttgc ggcgattatg
     2521 atcggaccca tccgctgatc gacgggtcgg tgaaaccgga agggattgaa ctcaactggt
     2581 tggtcttgcc ccatctggag atctggacgc gcatgctcaa ttactatgac ttcgacgcgt
     2641 cggaaatttc cttgtcgtcg tacatcatcg cgcggagcat cggcaagccg ctgaccgcta
     2701 taccggtgtt tccggcgcgg gcgtttcgcc attcctatat tttcatcaac acaaagagcg
     2761 gcattcgcga gccgaaagat ttaatcggca agcgcgtcgg cttggccgag tttcagcaga
     2821 ccgcgaccgt gtggattcgc ggcacactgc agcatgagta cggcgtcaat ctcgacgata
     2881 tcgagtggtt cacctgggtg ccgcactccc gtatggagat ggaactgccc aagcgctaca
     2941 aagtcacgca tctgacggcg gacaaaaagc cggatcaaat gctattcaac g
//
LOCUS       DATIFR010000184         1280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1460325, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000184 DATIFR010000000
VERSION     DATIFR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460325"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1097)
                     /locus_tag="VLD60_10510"
     CDS             complement(join(256..713,713..1097))
                     /locus_tag="VLD60_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612206.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSB45436.1"
                     /translation="MWTDENRGRYDRSSLRYPSDLTNDEWAIVGPLIPGAKRGGNKRT
                     VVEREIVNGLMYVLSTGCQWAAIPKDLPPRSTVNDYFRRWDDDGTLDRIHHSLFVKCR
                     ELADREASPTAAIIDSQSVKSAEKGGPSIDPHGYDAGKKIKGKKRHVLVDTQGLLLHA
                     IIHSADVQDRHGGVLVMSTLFGLYPFLLKLYADGGYQGAQFQQGLKRVCRQVSVEIVK
                     RSDAAKGFIVLPKRWIVERSIAWLNRCRRLAKDWECLNRSALAFLRWASIRLMVRKLC
                     HTTI"
BASE COUNT          256 a          370 c          372 g          282 t
ORIGIN      
        1 gatagaacgt gacccgacat tatggatgaa gggctggaac cgcgagcgat tgcgtgtagc
       61 catccgcaat tgggtcgata aatatgggac caggtcgtta gctgtatttg ggatcagagg
      121 gtgaaattcg tgctggaggc cccggcaaag ttcggcgtca gtggcggcat aggatttttg
      181 cttgggcttg ccgctgtctg gtggattgcg ccgacgacag cagggggtac agttcttctt
      241 agagtctgtc cgggatcata tggttgtgtg acagagcttt ctgaccatca gacggattga
      301 cgcccagcgt agaaaagcca aggcgctgcg gttgaggcac tcccaatctt tggccagcct
      361 tcggcatctg ttgagccagg cgatagatcg ttcgacaatc cagcgcttgg gcaggacgat
      421 gaagcctttg gcggcatcgg aacgcttcac gatctcgacg ctgacctgcc ggcacacccg
      481 cttcagtcct tgctggaact gcgcgccctg gtagccgccg tcggcgtaga gcttgagcag
      541 gaaaggataa agaccgaaca gcgtgctcat caccagcacg ccgccatggc gatcctgaac
      601 gtcggccgag tgtatgatgg cgtgcagcag caggccctgg gtgtctacca ggacatgacg
      661 cttcttgcct ttgatcttct tgccggcgtc gtagccatgc gggtcgatcg acgcccccct
      721 ttttccgcgc tcttgacgct ctggctatcg atgatcgcag ccgtcgggct ggcctcccgg
      781 tcggccaact cccgacattt gacgaacagc gagtggtgga tacgatcgag cgtgccatca
      841 tcatcccagc ggcggaaata gtcgttcacg gtactgcgcg gcggcagatc cttggggatt
      901 gccgcccact ggcagccggt gctcaggaca tacatcagcc cattgacgat ctcccgctcc
      961 accaccgtcc gcttgttgcc gcctcgcttc gcgcccggga tcaacgggcc gacgatcgcc
     1021 cactcgtcgt tcgtcaagtc gctcgggtag cgcaagctgc tgcgatcgta acgaccacgg
     1081 ttctcgtccg tccacatggc ggttcctgca aatcggtccg cctccatgga atcataaatg
     1141 attccaacga atcaagatgt tcccggatag acacttagaa gatagcgagc tggccggctt
     1201 tcttctgttc ttgccgttga taatgtaggc ctaacactgc cttatacatg acacgagcca
     1261 acagaaacac ctgatcaatt
//
LOCUS       DATIFR010000185        39332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_696385, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000185 DATIFR010000000
VERSION     DATIFR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 39332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39332
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696385"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VLD60_10515"
     CDS             <1..254
                     /locus_tag="VLD60_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSB45437.1"
                     /translation="DIGLAFQIADDVLNVTGTREELGKNPNTDAERGKKTYPTFYGVD
                     GAMKLADDCVTRAIGRLQSFGPSADPLREIARYITSRKN"
     gene            365..1348
                     /gene="hpnA"
                     /locus_tag="VLD60_10520"
     CDS             365..1348
                     /gene="hpnA"
                     /locus_tag="VLD60_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HSB45438.1"
                     /translation="MKALVTGATGFVGGAVARALVRTGIDVRVLARPDSDLQNLEGLA
                     VERVAGDLRDPASLRAALTGCRQLYHIAAHYALWAPEPSIFYDINVTGTRMILEAARD
                     VGIERMVYCSTIGAIGLPPGGGPGTEETPVSLDQMAGHYKRSKYLAEQEVLRLAREGL
                     PVVIVNPSAPVGAGDVKPTPTGQIIVDFMKGRMPAYIETGLNIIDVDDVAFGHLLAMQ
                     KGRVGERYILGNKNLMLREVFEILSRLTGVKAPSIKLPRMAILPLAYLNHWIANLTGR
                     PPRIPLEGVKMAKYKMHYDCSKAISELGLPQTPPEVALEKAVRWFREHKYA"
     gene            1419..2285
                     /locus_tag="VLD60_10525"
     CDS             1419..2285
                     /locus_tag="VLD60_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HSB45439.1"
                     /translation="MELFSLFLNTVVLRPYVFLFLCAFFVFAMQLIGWPRTWRFWLIS
                     WGTAFICEFSSTRTGIPFGWYHYNGSTVGQELYISNIPFMASISFSFLLYAAYSVTLC
                     VLLPIDRTSTPGHPPLIPLHFDPASRSSRRVLVSTALFFALLDMVIDPLALRGDRWFL
                     GSIYLYADPGVHFGVPLTNYLGWVVVGLISLSIYFRFDRRLPDPPRHTHWSTTHRLLP
                     GVGLYYAVLAFNLGLTFWIGEWHMGVSGVLMYLPITVWLALRLTGSLRPLESYAMERS
                     TRPGSNQGRDFV"
     gene            2298..2915
                     /locus_tag="VLD60_10530"
     CDS             2298..2915
                     /locus_tag="VLD60_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB45440.1"
                     /translation="MKAKLIGLIVIMVLGTLGAFGWVGYQLFTTGFSAKTEPHVLEVM
                     MARQIRHLAIPIEHRNRPNPVPFSPAVLKEGLAHFADHCATCHSNDGSGRTPIGKNVY
                     PKAPDLRLTDTQSLSDGELFWVIHHGIRFTAMPAWGEGEPDKDVDSWKLVHFIRHLPQ
                     LTQDELDEMKTLNPKTRHEIEEAAAFEKFLQGDDTAAGQTGSHLH"
     gene            2950..3303
                     /locus_tag="VLD60_10535"
     CDS             2950..3303
                     /locus_tag="VLD60_10535"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HSB45441.1"
                     /translation="MVQYVILAFVLFFTGATAFAHGTGQHVLGTVTAIDSTHLAIKTP
                     KGATVDVQINKDTRFKEKGNPKGTNLPAVGDRVVIEATKDNKVLTATEVHFSAAKRVI
                     PAAPAPAQPAPAPAN"
     gene            3321..3779
                     /locus_tag="VLD60_10540"
     CDS             3321..3779
                     /locus_tag="VLD60_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSB45442.1"
                     /translation="MRIVHFSQQMQASPFSGGLQAVAVRILITGIAVFLALATVPGLE
                     ADSFGAGLAAVLVLTILNLILRPILLLLTLPLIVLSLGLFLVILNALLLEFTAYLVKG
                     FTVSGFWSAVGGALVISIVTAILNMMATDRRQDEIHSSDPRQPKIINPSE"
     gene            3849..5675
                     /locus_tag="VLD60_10545"
     CDS             3849..5675
                     /locus_tag="VLD60_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45443.1"
                     /translation="MTPTATHSKPAPETQLQRTLTTVLNELPVDSALAAIFHRENGPL
                     TGHAARGFTPRDVQAILRTLSAPALVAAGSSHDQEGGKTMRLRLITPGAKSLLGVPLK
                     YRNRTYGFLVIGRKEGAAFTKKEKGLMDQASDDVTKALDRDGLFDLNAVLSRPLVTSE
                     PVPAAPSAAEMFAAPVSQLTPQLQEQITAVLTDANQIVPYDRAWVCHYDPIAGNVEVL
                     GMAGDLKGDQKDSKKDMKPGHRLTLDSSAAGWAIRHRKPRVDHDLASTQGRFLDHKQL
                     FKDRFQSSLVFPFFVRGQVGGTLTLASREAGRYQPTDARTLEPTILKLADLLQAPMPA
                     ATPPAPAVEAGGVPSPSETQAAVPLEPIIRKQERQAAIGEFSAFLATEVREPLGSIRS
                     QLEEVTGEGILDFDPQTRVENAMRDLIRVEAILNEILDFAKPLELNRRLVRIPEVIES
                     ALTVVATDLEVTRIHVAKEYAPILAPVRGDEAKLQQVFLSIFKNAGEAMTPGGTLTIH
                     VTQHRAGRGVEDQILIKNDGAPIPPEIVDKVFEPFFTTKKAGTGLGLATVKKIVEEHG
                     GSIAIASAPGEGTSVTIRLPGVSRGPAFRHRGRGRRPPRRGN"
     gene            5879..6247
                     /locus_tag="VLD60_10550"
     CDS             5879..6247
                     /locus_tag="VLD60_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45444.1"
                     /translation="MKLVFGTVATVSGVLFALSMASANPALLPKHEGYPMKNSGSPVT
                     GQPTANDPGQSDARGESTLLKSAAFDDKHAKQELKKTDNERITANEGAGRLPKVQGPQ
                     IQIAPPVTSATKITGDRKIE"
     gene            complement(6330..6884)
                     /locus_tag="VLD60_10555"
     CDS             complement(6330..6884)
                     /locus_tag="VLD60_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSB45445.1"
                     /translation="MRAILPQTYFSRSTLTVARSLVGKYLVRENGKGIVAGKIIEVEA
                     YIGPQDKACHASKGRTARTDVLFGPPGISYVYLIYGMHHCLNVVTERSEFPAAVLIRA
                     LEIDGLLIDGPGRLCRALDIDRSLNRIDLTSGVHLWFEDRGEKIPRGVIGTHPRIGVD
                     YAGVWAMKPWRFRLSQRGPRRPTK"
     gene            complement(7039..9093)
                     /locus_tag="VLD60_10560"
     CDS             complement(7039..9093)
                     /locus_tag="VLD60_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSB45446.1"
                     /translation="MSRIVRAAILLWWILHSPSLSVAHDPDAPDIEVPEVSVVGERPV
                     AASSQQFIPDKEILLQPQGRPAQVLRLIPGFIAVEHSGGAGKADQYFLRGFDADHGTD
                     VAFFIDNMPINLRTHGHGQGYTDLNFIIPETIQGIDVVKGTYHAEFGDFATAGAVTFK
                     TRELLQENVVQGAGGQFDTQRYLLMLSPTRDNVRTLIAAEGYYTNGPFENDNRYFRFN
                     LMGKATVNPTVRSELSVTGSYHKANWNASGEIPFRAVADGTIDRFGSIDPSEGGKTER
                     ATGRTQYHYDIPSGGQFFADAYGMYYKLDLWTNFTFFLDNPVNGDGINQQDQRYMYGG
                     NLGYRQTGEIFGIQSTATAGFQVRNDSISDIRLGTQTKRTSTGTIVQNAVQEASYSPY
                     MKLELQPTPWMRLAGGVRADYFTFDVRNLCDTCPEQPAGRKDSGQISPKGNLILGPWF
                     NTEFFVNYGTGFHSNDARSTIAGDSSPLARAQGIEVGLRSRPWGQDRMELTATFWGLD
                     LKSELVFVGDAGTTEARGPTRRYGAEAGARGQIYGPLYFNGSFTWTHAEFKDTGLAIP
                     LAPELTAYAALLLRWPEGLSSQLQMTYLGVRPLTEDRTINAPSWIDFDLTERYQLPVK
                     LSYGRLEAFLFVQNLFDTQWEQATFAFQSRLPGEPAGGVTDIHFVPGNPRFVMGGLAW
                     YF"
     gene            complement(9102..9524)
                     /gene="nikR"
                     /locus_tag="VLD60_10565"
     CDS             complement(9102..9524)
                     /gene="nikR"
                     /locus_tag="VLD60_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HSB45447.1"
                     /translation="MKKLVRFGVSLDHHLLDDFDKVVRRRKYATRSEALRDLIRDQLV
                     SQEWSSDGDGEAVATITFVYDHHVRDLTRKLTHIQHNFQGHIMAGMHVHLDHDHCLEV
                     LVVKGKVSQIRHVADALVSVKGVKHGKLTMTTAGKGLS"
     gene            complement(9641..11038)
                     /locus_tag="VLD60_10570"
     CDS             complement(9641..11038)
                     /locus_tag="VLD60_10570"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSB45448.1"
                     /translation="MVRRILCAGLCLLTLSMAGGAWASGRLISVSGGGDLQNQVKATA
                     SKVIPAVVSIASTVMVRDQAFSDETLPFGLFKEPPARRQYGQGSGVIVSPDGYIITNN
                     HVVADAVDVEVILADRRQYKGKVVATDPKTDVAVVKIQGTNLPAVAWGDSSNLAVGDF
                     VLAIGNPLGLSRTVTFGIVSAVGRADVGVADFEDFIQTDAPINPGNSGGALVNINGEL
                     VGINTAIASPTGGSVGVGFAIPSNMARAAMQSLIKTGRVVRGFLGASTQDVTPLLGKI
                     FRLPDVKGAIVTDLQAKGSAEKAGLKRGDVVERFDGRDVMDSGHLRNLIAASAIGSKH
                     RLDVVRDGKTIQVELMVQEAPRERAKKSQAAAAVSTSHPLAGVVFDDVTPPLARQMDL
                     AVNSGVVVTDIEEGSLAESSGLQPGDVILELNRQPVPNFSTFQRIADPMKPTDLALLL
                     VNRQGTVLYIPVRGE"
     gene            complement(11119..13659)
                     /locus_tag="VLD60_10575"
     CDS             complement(11119..13659)
                     /locus_tag="VLD60_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSB45449.1"
                     /translation="MAIDPVCGMTVDPATAAGRYEYKGATYYFCALSCLETFKADPEG
                     ALRPASAGLITLGSKKSLPMMMPAPVIGGAGDVDPVCGMTVQPTSAVGSHVHAGKTYY
                     FCSQSCLEKFRKDPTRYLTPPAQRLPEPVTVPPGAAIEYICPMDPEILESKPGACRIC
                     GMALEPKVVSLEDSPNSELDDMARRFWVSLGPALLVMALAMSDMIPGQHLKDLLGGSR
                     LNWIQWLLATPVVLWGGWPFFERAWKSILNRAPNMFTLIGIGTGAAYLYSAVATSVPD
                     LLPASFRQHDGSIAVYFEAAAVITVLVLLGQVLELRARSQTTSAMKALLRLAPKTAKR
                     LESDGREVDVPIEQVRVGDRLFVRPGERVPVDGVVVEGATSVDESMVTGESLPVEKAI
                     GAAVTGGTINGTGSILMQAQRVGRETLLARIVQMVSEAQRSRAPIQRVADVAAGYFVP
                     LVVAASFVTAVAWAVWGPEPRLAYALVNAVAVLIIACPCALGLATPMSIMVGTGRGAM
                     AGVLVRKAEALEVLGKVDTLVFDKTGTLTEGKPRLVAIRFVPPWNEADVLRFAGSVER
                     KSEHPVASAVVASAEERRLLLSPVQEFRSVTGYGVSGVVEQHQVAVGTPTLLREQAAV
                     TDQGLAALEEEATRLRQEGQTTVFVAIDGKAAGLLGVADPIKPSTPEAVRILGAQGIR
                     LVMVTGDHVHTAEAVARRLGISEVHASVMPDQKGRIISHLKSQGRVVAMAGDGINDAP
                     ALALADVGIAMGTGTDIAMESAGVTLVKGDLRALVRARRLSQATMRNIRQNLFFAFVY
                     NMVGVPIAAGVLYPLWGLLLNPMIASAAMTFSSLSVISNALRLRHVEL"
     gene            complement(13680..14336)
                     /locus_tag="VLD60_10580"
     CDS             complement(13680..14336)
                     /locus_tag="VLD60_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB45450.1"
                     /translation="MENSVLMKGMAIGSWMLLVGLFMGMVPVHAASPELKGKFEILKE
                     EKSTHSPGKVKLVEFADFYCPHCHHFEEAGLPLIQKEFGNKLEATMVGFPVFRNKLPT
                     PFDMYEQAKMMGKGNEMKKVLFRTIHQDKMTGVLDRSLREALIKEVGLDPKAFEEGMA
                     TGKPAKAFDEGRQWGERIKVQYTPTLLLDGNIKIEGDNMKTENIITVIRSILESDSKK
                     "
     gene            14419..14958
                     /locus_tag="VLD60_10585"
     CDS             14419..14958
                     /locus_tag="VLD60_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSB45451.1"
                     /translation="MHLRPFLVSFVLLLVFTGCASTKEAGDGSAANQITFPQVKEALD
                     SFKGQTVMFGGQVLNARRLKEGTRIEILQLPLDRSGHPGYDLTQSQGRFVALHRDFLD
                     PATLPYGTLVTVTGQVAGSITLPLDETEYTHPIIDITQLRVWTHVQDFAPCMRPCAWP
                     GPYWGPYWGPYWGPWPYRW"
     gene            complement(15050..16108)
                     /locus_tag="VLD60_10590"
     CDS             complement(15050..16108)
                     /locus_tag="VLD60_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45452.1"
                     /translation="MKALARRYFHLVIGVWLGLLPIAPIQATPPADTGKRAIERAADL
                     YKSGDLAGASEAARKAIELNPSSAEAHHLLGLVYFKEKKPAQAVDAFTHALKLKPAYP
                     DALNDLAEVYLAQGKTAEAEQALKRAIDLDPHHEDSYEDLAKLYESRHDVSASMKTYK
                     SLLAIRPEHPDALFGLAMLYERQGDNKAAREMLGRLTKANPKHADAWYQVGRLAERNN
                     DLIEAAYAYRQAIAAKPALVDAHYNLGFILRSQNKPVEAEKEFLEVIRYRPEYAEAHM
                     NLGVIYTSMNKLEDAEHEYEKAVELKPDYAEAHYNLGVFYELHRKDMPRALAQYHKYR
                     NLGGRDDRVERIIGSSGR"
     gene            complement(16300..17421)
                     /locus_tag="VLD60_10595"
     CDS             complement(16300..17421)
                     /locus_tag="VLD60_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45453.1"
                     /translation="MNLLNKLIPIVTFAWITGLMAFVSPVGASCGAVTCFVVIGSQQQ
                     VPQEGLLTVNGIYNYTPMRLLDGTSGVIPAVDQGSRQMILNHHQETRTITQQATLDIN
                     YGVTDRFGLQITLPYMWRTHKHIDGLGEDGVAGEGAPTQFSSDGIGDMRVGLKYNVLP
                     TLRSMVVLGFGVYLPTGNTHAHDSAGELMESSTQLGRGQVGLNPTIYQTYELIPHRLN
                     QFASASYRHTFRNNYGYQFGDEYMLNAGFNLVTTPWLILTGQVNYRYLVHDNFSSSLS
                     RSATPADAPDFPGEPVVLDPTIKNRAVPTTGSTYHAFSPGFQVNLGQLIESKWTNMTS
                     LYFFAQIPFARDSNDNLAQGTSYVFGLTKSFQLMKPAGG"
     gene            complement(17578..17886)
                     /locus_tag="VLD60_10600"
     CDS             complement(17578..17886)
                     /locus_tag="VLD60_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45454.1"
                     /translation="MVALKRSGLVWSLLLCIVLVNGFMAAPSVAHAAKHGSHQAGTHS
                     SGLCAWLCAAGQGIETSSVSLESTLRLVDRVLSFHVDQYAASFSSFTFLRGPPASSAS
                     "
     gene            complement(17896..18459)
                     /locus_tag="VLD60_10605"
     CDS             complement(17896..18459)
                     /locus_tag="VLD60_10605"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB45455.1"
                     /translation="MNTFLRRYVSNIRLSLVLAASLVALATGVAWSMGSRVPVVGTPA
                     EDFQLVDLSGKTQSLSQHRGKIVLLNFWATWCKPCTTEMPAMQTTYDKLRDKGFVVLA
                     VNELENDAKVREHIQQYGHTFPVLMDRDNKVANQFGVFGLPVSVFIDEQGIVQEYIKG
                     GLLTEQKIYDVVVRIQKQAPVKAASLR"
     gene            complement(18585..18887)
                     /locus_tag="VLD60_10610"
     CDS             complement(18585..18887)
                     /locus_tag="VLD60_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45456.1"
                     /translation="MTHSFLQRSLACLLVLCVLVIGGLASAQSITHESHHAHHQKATH
                     GTVLCSWMCAAGQAGEAAVQFVPVALMPHDFVEFTSTDQVPTIFSGILATRAPPVR"
     gene            19072..21792
                     /gene="secA"
                     /locus_tag="VLD60_10615"
     CDS             19072..21792
                     /gene="secA"
                     /locus_tag="VLD60_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSB45457.1"
                     /translation="MLTQLLNLVFGSKNDREIKALMPIVQRINSLESGLTPLSDQALA
                     DKTQEFKKRLSAGETLDDILPEAFAVCREMSRRKLNMRHFDVQLIGGMILHKGRIAEM
                     KTGEGKTLVATLPIYLNALEGKGAHLVTVNDYLAKRDAQWMGQLYHALGLSTGIIQHD
                     ASFIFDPTYDASDRRLQNLRPCTRPEAYRADITYGTNNEYGFDYLRDNLVVTDLNQCV
                     QRDLNFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINAIIPQLKAEQDYTIEEK
                     TKTASLTEDGNVRVEKLLGVDNLYDPANMDLVHHVVKALQAYALYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLGGMT
                     GTADTEAAEFAKIYNLDVNVVPTNRKMIRLDYADVVYRTEKEKFAAIAEEIKECHERG
                     QPVLVGTISIEKSEKLAGILSRNGIKHNVLNAKQHEREAEIVAQAGRKGAVTIATNMA
                     GRGTDILLGGNPDFMYKQVLYREENLPDSRKLEVFEEIRSDCEKNKQEVIAHGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGTSRFYLSLEDDLMRIFASERVSQLMLKLGMEEG
                     VPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYQHRRAVLAGANLT
                     EDIYDMMTDLVESSLNIYCPAEQYPEEWDMKGLTEMMHGQFGVDITQGKHDGGESLRD
                     MGRDALLEDLKAQVRDAYGRKEQELGSDLMRFLEKNFMLQVIDHHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIEYKREGFELFAGMMERIKSDTLDRLFRVQAVRNEGEAAAPPPPP
                     IISHPQPKLTLNRSDEPVAAQTVHRSDDKVGRNDPCPCGSGKKYKKCHGA"
     gene            complement(22397..22906)
                     /locus_tag="VLD60_10620"
     CDS             complement(22397..22906)
                     /locus_tag="VLD60_10620"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HSB45458.1"
                     /translation="MTKSADKATADFAVAVDQVEGVCPAGEKWARQNMIEKKVPIFSC
                     EGPCIRGEIARLAANLVSREMPSYARCCYAEAALVPHSAMARWVKEAEKIVVIDGCFL
                     ECIGRVVNNLVDREKVIHIDALPLYKKYTDVFSMDDVPEEERKEVARSVADKIISKLN
                     EQSASKALP"
     gene            23702..24877
                     /locus_tag="VLD60_10625"
     CDS             23702..24877
                     /locus_tag="VLD60_10625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSB45459.1"
                     /translation="MTLGHPELVAAIASEITSSGPIPFVRFMELALYHPQYGYYMRPP
                     ESGAERIGWSGDFYTSSDVHPILGQALAKQAAQIDAKLGSPDPFTVVEMGPGKGVLSR
                     DFLKAIDSQYGELSRRLRYVLIERSPAMRTLQQQQLEPWLNQPGRLTWLEDLTSLAPA
                     SVTGLLFSNELPDAFPVHRIQMVGKEPQEIFVDYQDGRFVECLKTLSQPALRNHFQRL
                     RLELPDGYRTEINLQALSWMQLVAQSLARGVVITIDYGHSAQDLYGPDRSKGTFLCYY
                     SQMASDDPYVRIGQQDMTAHVDFTSLATVGEEGGLSVTGFTNQMSFLMGLGIEETISR
                     LDPESPQFRAAIHLLMPEGMGRTFKILVQHKGIERPELDGLAFKPFFGSALSLHAAA"
     gene            24893..25264
                     /gene="ndhC"
                     /locus_tag="VLD60_10630"
     CDS             24893..25264
                     /gene="ndhC"
                     /locus_tag="VLD60_10630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSB45460.1"
                     /translation="MGSEAVPLNYLPILLFIGIAIVFGAISLLAGWFVRPSRPYRAKL
                     NPYESGSPLFSDARIQFPMRYYIIAMLFVIFDIEVIFIIPWAVRFQSLGLLGLIEMLV
                     FIAILLVGLWYAWKKGALEWD"
     gene            25350..25829
                     /locus_tag="VLD60_10635"
     CDS             25350..25829
                     /locus_tag="VLD60_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSB45461.1"
                     /translation="MSFLERQFEANIITTNLDAVVNWARKSALWPMTFGLACCAIEMI
                     ASVSSRYDLDRFGAGVFRASPRQSDLMIVAGTVSRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYVPGCPPRPEALMDGLLKLQEKIQREKVFVK"
     gene            25918..27663
                     /gene="nuoD"
                     /locus_tag="VLD60_10640"
     CDS             25918..27663
                     /gene="nuoD"
                     /locus_tag="VLD60_10640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSB45462.1"
                     /translation="MQPLIETLMTRFPKAVMSVEADTARSEVSILVAAEHLVELATYL
                     HDAPEASFNHLTDICSVDYPEDRQRFEVVYHLHSLPHRRRLRLKARLPEDNPTIASVT
                     GIWKGAEFLEREVYDMMGITFSGHPDLRRILMPEDYAEGYPLRKDFPTEGRGWRSQFD
                     FIPRLDEPPGEAAGSELREEQKQAFRAEPSGSRRREELLLNMGPQHPSTHGVLRVVLE
                     LDGERIVKATPDLGYLHRGVEKLSEGLAYMQIIPHTDRLDYVCAMANNYAYVRAVEKL
                     LGITIPERAEYIRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREILLDMFEK
                     LCGARLTLNYYRIGGVDSDFTPDLVLRLKTFLDTFPDKVKEYDSLIASNRIWLGRTKN
                     VAVISPEDAINFGCTGPVLRGSGVAYDIRKEEPYGAYDKVDWEVPVGKNGDTYDRYWI
                     RMEEMRQSARIIKQCLDQMPQGPIIADAPQYIPPPKQLVMRDMESLIHHFIIFTQGLK
                     PPKAETYCATEAPKGELGFFIVSDGSARPYRLKIRSPSFVHMGAFDHMARGYLISDII
                     TIFGTYDIVMGECDR"
     gene            27764..28303
                     /gene="nuoE"
                     /locus_tag="VLD60_10645"
     CDS             27764..28303
                     /gene="nuoE"
                     /locus_tag="VLD60_10645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HSB45463.1"
                     /translation="MLKEKYQREIDELLSRYPVKRSALIPLLYLAQQENGYITEAAMI
                     EIAGLLKLTPPQVYETATFYTMLNLKKVGRFHIQVCKSLMCALVGSDTVIDWIKTKIG
                     IGPGETTPDGLFTLTAVECLAACGTGPMMQINDDYYERLTEEKVDRILADLKSTGTSS
                     LKSGPFMWPEPVNVVRRET"
     gene            28371..29678
                     /gene="nuoF"
                     /locus_tag="VLD60_10650"
     CDS             28371..29678
                     /gene="nuoF"
                     /locus_tag="VLD60_10650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSB45464.1"
                     /translation="MPKHEPVLLKNMLQPGYTGSLVDYEKAGGYQALRKVLGKIAPGD
                     VTQLVIKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLIERD
                     PHQMLEGIVLACYAIGAESAYIYIRGEFVLGAQILERALGEARATGYIGKNILGTGVS
                     VDVWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHIISRGPEWFAAIGSPPKSTGTRIFCVSGHVKRPGNYEVPMGIPFRELIYEHAGGM
                     RGEKPLKAFIPGGASAPFLTPDHLDVKLDFESVAAAGSMLGSGGVTVMEEGTSMVWAA
                     LRLMEFFYHESCGKCSPCREGSSWLVQTMRRIMAKRGQAQDLETLTDLCKNIAGRTVC
                     AFGDAEVAPIMSTMKHWRHEYIALIHEAEAANLLRPEPALKRH"
     gene            29681..32350
                     /gene="nuoG"
                     /locus_tag="VLD60_10655"
     CDS             29681..32350
                     /gene="nuoG"
                     /locus_tag="VLD60_10655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HSB45465.1"
                     /translation="MTDSTTDTVRLTIDGIPITVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEVEKMPKLQTSCSTVATEGMAVRTATTVVNDAHKSVLEFILANHP
                     LDCPVCDQGGRCDLQDFSHQYTATASRFIETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEVMDVKALAPAGRGTMTEIKSFGTHQLNCEFCGGCVQICPVGAITSRLSMYEYR
                     PWMLKRADTICQYCGDGCQITVQTKDNELIEVMSAQGAGRNNGDLCARGFFGYHAAAH
                     PDRLTKPLMRRNGALVETTWEDALEYTAEQISRLKTSHGAQAFGGLISSRCTNEELYL
                     FQKVMRTAIGTNNLDSSARYGQLNAVQAMRRIQGTHRWTVTFEDIIQADVLLLVGTNI
                     TEANPIAGLKVKEAVKKHQARLFTIESIQPAVDTISNITNLAELHICVPPAQYDRAIL
                     GLLKAAVEGQQVDQTVHNPSSEFVTAVSQALARLSWPDIEAGTGTTRETFVQLAAGIA
                     KGRRVVVLAGQDLLRSAGGYGICLNLLDLLLLTGKLTESGCGFAPLAEENNDQGAVEM
                     GAIAELLPGVLDVANQEARAQMGRAWKTDLPLAAGATLVEMIDRAHSGLLKGMLVVGE
                     NPVGSLPPHLEVGEALRKLEFLVCQELFLTETASLAHVVLPASSSLEKAGTFTNEEGH
                     VQAVRPTIEPVGESRPDWEILSAISMLLGAPRDYGDAKELLTEIRSVVPGYGLLGPGP
                     IPPKVDRAAIERYVTGGYRQDLAGRYTLATHAMPLEGTMELRLVQSLFHSGKFSTRSK
                     GLLQIEARASLRMNPVDAARLTLGDGDRVRLSNQRGDLKATVKLLERVPQGALWFPDH
                     FAQEAANLFECAIDPHTKVPSIRTAAVSIVKIT"
     gene            32399..33469
                     /gene="nuoH"
                     /locus_tag="VLD60_10660"
     CDS             32399..33469
                     /gene="nuoH"
                     /locus_tag="VLD60_10660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSB45466.1"
                     /translation="MTELSLRLAVSLAQIAAVMGIVMLTVMILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPIADGLKLFFKEDIVPSGANKFMFSLAPILAMVPALIGFAVIPFGP
                     SHKFEIFGITVEPFVISDINIGILYILAFASIGAYGIILGGWASNSKYSLLGGLRSAA
                     QVISYELNVGLAIVGVLILAGSLSLVKITEAQSGGFWHWYVFALPAPQIFAFVVYVIS
                     AVAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMVLVSCVAAAMFLGGW
                     NAPYPGTILGHIGLEQLAWVENIAWFTVKAYFFLFLFFWLRATLPRLRYDQLMKFGWK
                     VMLPIALGNIVVTSIAVYLFPR"
     gene            33500..34042
                     /gene="nuoI"
                     /locus_tag="VLD60_10665"
     CDS             33500..34042
                     /gene="nuoI"
                     /locus_tag="VLD60_10665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSB45467.1"
                     /translation="MQRFTAWLKTIVFYELLVGMKATMSHLLRYRPITLQYPHEKRML
                     PDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRYAKEYYMDM
                     TRCLFCGMCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGDRSFPVREKRLEL
                     QHPNVAFFNVAFGHVPQKPN"
     gene            34128..34649
                     /locus_tag="VLD60_10670"
     CDS             34128..34649
                     /locus_tag="VLD60_10670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSB45468.1"
                     /translation="MMEQVFFFYFALVIAVSSMLVVALRNPIYSALSLLIMFFHVAGL
                     YVTLHAEFLAAVQVIVYAGAILVLYLFVVMLLNLHQDDRYHQQWPVAAVIGLTLVVEA
                     VMLSLVKGQAIPPAPANTETAVEGIGNTEALGDVLYSTYLFPFEVASLILLVAMVGAI
                     ILAKRDLLEPKES"
     gene            34646..34951
                     /gene="nuoK"
                     /locus_tag="VLD60_10675"
     CDS             34646..34951
                     /gene="nuoK"
                     /locus_tag="VLD60_10675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSB45469.1"
                     /translation="MTIPLSYYLILSAIVFLTGVVGVLIRRNIIIILLSVELMLNATN
                     INFVAFSEYFHNVAGQVFVFFALTVAAAEVAVGLAIIIALHRAKSTINVDEFQLLKW"
     gene            35047..36945
                     /gene="nuoL"
                     /locus_tag="VLD60_10680"
     CDS             35047..36945
                     /gene="nuoL"
                     /locus_tag="VLD60_10680"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSB45470.1"
                     /translation="MTYELIPLLPLASFLILGLFGHWIKDKAHLVAVPAVVLSFLFSI
                     FAFIDVADGHHTTFRLYTWLTSGTLDIHIGLTIDRLTAVMLLLVTTVSALVHIYTIGY
                     MQGEPGYARFFCYIALFTFSMLMLVLADNFLQLFVFWEAVGLCSYLLIGHWYERPSAC
                     AAATKAFIVNRVGDFGFLLGLLLIWTTFNSLDYSDVFGKVHDAAGRMINVLGPLGGSW
                     DVPVLTVICLLLFTGAIGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFLVAR
                     LAPIYNLSPAAMTVVAIVGALTMVLGATIALTQTDIKRVVAYSTISQLGYMIMACGLG
                     AYASGIYHLLTHGAFKALLFLGCGSVIIALHHEQDMRKMGGLKDKLPVTYWTFVIGSL
                     ALAGFPLTAGFFSKDELLVSAWSSGTLGQVLTVLGLLTALMTAFYSFRLVFVTFWGPS
                     HVDHHHAAHVHEPPLDMTVPLIVLAALSIVTGYLGIPEFLEPMFASESGGGSNHGSGA
                     IFIMIAATAMGLLGIAGAYYVYVLNPSLPDRWARQWQAAYQLSLNKWYVDEAYDRSIV
                     RPTFDAATGLWKGIDVAIIDGTVNGVARAIAWGGWLLRLIQSGQTQHYALGMALGAVV
                     VLTLFMMF"
     gene            36952..38514
                     /locus_tag="VLD60_10685"
     CDS             36952..38514
                     /locus_tag="VLD60_10685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB45471.1"
                     /translation="MQSGGFPWLTLLILLPLAGASVLYLAKDSSARLIALAVTVADFL
                     LALPLWWLFDPSSSQMQFAEHVTWIHSPPIHYSLGLDGISLPLVLMTAALMPLCVLIS
                     WDAITTRIRGFMAMLLLMEAAMLGVFVAVDFVLFYVFWEVMLIPMYLLIGVWGGPNRL
                     YAAIKFFLYTLAGSVLLLVAILALYFYGGHTFDILALSQVTYPASLQMWLFLAFFAAF
                     AVKVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFSLPMLPDASRDMTPV
                     MALLSIVAIIYGAYMALAQTDLKKLIAYSSVSHMGFVTLGLFVLNQQGIEGAVLQMVN
                     HGITTGALFLCVGIIYERTHSRMIADNVGLAKPMPQYATLLVIFALSSLGLPGTNSFV
                     GEFLVLIGTFLWSKTATAFASLGIILAAAYLLWMVQRVAFGIPSPHHLPNLKDLSRRE
                     WATLAPLVVLVFWIGLYPNPLVSRMHASVTKTLDAMARTKVAPISHPSAAHPLPVAVT
                     SRDLQIEESSKP"
     gene            38511..>39332
                     /locus_tag="VLD60_10690"
     CDS             38511..>39332
                     /locus_tag="VLD60_10690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSB45472.1"
                     /translation="MTSYGTDLLVILPELIIVAAACLVLALDPVTPSSKKHLLAWLSL
                     AALALCIGLTGGQISVLNMRVFAFSDLVVVDGYARFWKLLLYGVTGLTILMSLPYLKA
                     ERLHLGEYYGFILLALSGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRSSPRSLEA
                     SAKYFVLGAFSSGLLLYGISLLYGFAGSTKLVSIAAAIGTRGFEDPMILIATILLVVG
                     FGFKLAVVPFHMWTPDVYEGAPTSVTAFMAVASKAASFGAFLRVFVEGLGGLKADW"
BASE COUNT         8268 a        11749 c        10919 g         8396 t
ORIGIN      
        1 aagacatcgg gctggcgttt caaatcgccg acgatgtgtt gaacgtcaca ggcacccgcg
       61 aggagctggg caagaatccc aacaccgatg cggaacgcgg caaaaagacc tacccgacgt
      121 tctacggcgt cgacggcgca atgaaacttg ccgatgactg cgtcacccgt gcaatcggcc
      181 ggcttcagtc attcggccct tccgccgatc cactgcgaga aatcgcgcgg tatatcacct
      241 cacggaagaa ctgactggta aggggtgagg cgtaaggggt gaggagagat gcccggatga
      301 accgacggtt agaattaagt ttcacccaac cccctacccc taaggcctca cgctccacct
      361 cctcatgaag gctctcgtca caggtgccac cgggtttgtc ggaggtgcgg tcgcacgagc
      421 gctcgtccgt accggcatcg acgttcgcgt gctcgctcgt cctgactcgg atctccagaa
      481 tctcgaaggc ctggccgtcg aacgagtggc aggcgacttg cgcgatccgg cctccctccg
      541 cgccgccctg accggctgcc ggcagctcta ccatatcgct gcgcactacg cgctttgggc
      601 cccagaaccg tcgatcttct acgatatcaa tgtcaccggc accaggatga ttctggaggc
      661 agcccgcgat gtcggaatcg aacggatggt gtattgcagc acgatcggcg ccatcggcct
      721 cccgccaggc ggagggcccg ggaccgaaga gacgcccgtc tcgctcgatc agatggccgg
      781 gcattacaag cgatcaaaat atctggctga acaagaagtg ctgaggcttg cacgcgaggg
      841 cttaccggtt gtaattgtga acccaagcgc gccagtcggt gccggggatg tcaaaccaac
      901 cccgaccggg cagattatcg tggatttcat gaaaggccgt atgccggctt acattgaaac
      961 cggcttgaac attatcgatg tcgatgacgt cgccttcggc catctgctcg ccatgcaaaa
     1021 aggccgcgtg ggcgaacgct atattctcgg caacaaaaat ttaatgttgc gcgaagtgtt
     1081 cgagatcctc agccggctga ccggagtcaa agcgccatcg atcaagttgc ccagaatggc
     1141 gattctcccg ctggcctatc tcaatcactg gatcgcaaat ctcacaggcc ggccgccgcg
     1201 cattccgctg gaaggcgtga agatggccaa atacaagatg cactatgact gcagcaaggc
     1261 gatcagcgaa ctcggcctgc cgcaaacacc gccggaggtc gcactggaaa aagcggtacg
     1321 ctggtttcgt gaacacaagt acgcgtaacg acgacgtgaa cgtcgccgaa tgataaatta
     1381 ggagcttctg ccgaggcact gctcacgact cagagccgat ggaactgttc agtcttttcc
     1441 tcaacactgt tgtcttgcgt ccttacgtct tcctgttcct ctgcgccttt ttcgtcttcg
     1501 ccatgcagct catcggctgg ccgaggacct ggcgcttctg gttgatcagc tggggcacag
     1561 ccttcatctg tgagttctca tcgactcgca ccggcattcc attcgggtgg taccactaca
     1621 acggctcgac cgtcggccaa gagctgtaca tctccaacat tcccttcatg gcttcgatct
     1681 ccttcagctt tctgctgtat gcggcctaca gcgtgaccct ttgtgtcctt ctcccgatcg
     1741 atcgcacttc aactcccggc catcctcctc tgatacccct ccacttcgat ccagcctcgc
     1801 gctccagtcg gcgcgttctc gtatcgaccg ccctcttctt cgcactgttg gacatggtga
     1861 tcgatccgtt ggcgctacgc ggagaccgtt ggttccttgg cagcatctac ctctatgctg
     1921 accctggggt ccatttcggc gttccgctca caaactatct tggatgggtc gtggtcgggt
     1981 tgatttccct ctccatctat tttcgcttcg accgccgact ccctgacccg ccacgtcaca
     2041 cccactggtc caccacgcat cggctccttc cgggcgtggg gctctactat gccgtgctcg
     2101 cctttaatct tggcctgacc ttctggatcg gagaatggca catgggggtc agcggcgtgc
     2161 tgatgtacct cccgataacc gtctggctcg ctctccgcct gaccggctcc ctccgcccgc
     2221 tcgaatccta tgcgatggaa cgttcgacca ggcccggtag taatcagggc agagactttg
     2281 tatagtgatt gaccagtatg aaggcaaaac taatcggcct catcgtcatc atggtgttgg
     2341 gtaccttggg cgcgtttggc tgggtgggct atcaattgtt cacgaccggc tttagcgcca
     2401 aaaccgagcc acacgtactg gaagtgatga tggctcgcca gatacgccat ttggcgattc
     2461 cgatcgagca tcggaatagg ccgaatcccg ttccgttcag cccggccgtg ctgaaggaag
     2521 ggctcgcgca cttcgccgac cattgcgcaa cctgtcattc caatgacgga agcggtcgga
     2581 caccgatcgg caagaacgta tacccgaaag ccccggacct gcgattgacc gatacgcaat
     2641 ccctgtcgga cggcgaactc ttctgggtga ttcatcacgg cattcgcttt accgccatgc
     2701 cggcctgggg cgaaggtgag ccggacaagg acgtcgacag ttggaagctc gtccacttca
     2761 tccgccacct gcctcagttg acacaggatg aactcgacga aatgaagact ctgaatccca
     2821 agaccaggca tgagatcgag gaagctgcgg ctttcgagaa atttctgcaa ggagatgata
     2881 ccgcggcagg tcaaaccgga agtcatcttc attgagtcat ctttcgtcta aaaggagatc
     2941 tgatttgcca tggttcaata tgtgatcctc gcatttgtat tgttcttcac tggagccaca
     3001 gcctttgctc atggaaccgg ccaacatgtc ctcggcaccg tcaccgccat cgacagcacg
     3061 catctggcga tcaaaacacc gaaaggtgca acggtggacg tgcaaatcaa taaggacacg
     3121 cggttcaagg aaaagggcaa cccgaagggc accaatctgc cggcggtggg cgaccgagtg
     3181 gtgatcgagg ccacgaagga caacaaagtc ctgacggcga ctgaggtgca tttttccgcg
     3241 gccaaacgcg taatacccgc agcccccgct ccagctcaac cagcgccggc accagcgaac
     3301 tagaccagaa ggattgactc gtgcggatcg ttcacttttc gcagcagatg caggcttccc
     3361 cgtttagcgg cgggttacag gcggtggctg ttcggatcct catcaccggg attgcggtct
     3421 tccttgcctt ggccaccgtt ccgggccttg aggccgacag tttcggcgcg ggactggcag
     3481 ccgtgctcgt gctcacgatt ctgaacctga ttctccgacc cattctctta ctcttgaccc
     3541 tgcctttaat cgtcctgtca ttgggactct tcctggtgat cctgaacgcc ttactgctcg
     3601 aattcaccgc ctatctggtc aaggggttca ccgtgtctgg attctggtcc gcggtcggcg
     3661 gcgcgctggt gatcagcatc gtcactgcca ttctcaatat gatggcgacc gaccgccgcc
     3721 aggacgagat tcacagtagc gatccgcgcc agccgaaaat catcaatccg tccgagtagc
     3781 tccgcctttt cgttgaattg cgatgcgcgc gttgctacaa tgcgcgcatc gcattccaac
     3841 accgattgat gacacccaca gctacgcaca gcaaaccagc ccctgaaaca cagctccaac
     3901 ggacgctgac gacggtgctg aacgaattgc ccgtcgattc tgcgctggcc gcgatctttc
     3961 atcgcgaaaa cggtccgctg accggccatg cggctcgtgg ctttacgccc cgcgacgtgc
     4021 aagcgatcct ccggacactg tccgccccag ccctcgtggc ggccggatcc tcacatgatc
     4081 aagagggcgg caaaaccatg cggctgcgtt tgatcacgcc tggcgcgaaa tctctgctgg
     4141 gggtacccct caaatacagg aatcgtacat atggtttctt ggtcatcggg cgcaaagaag
     4201 gcgccgcctt tacgaaaaaa gaaaagggcc tgatggatca ggccagcgac gacgtcacca
     4261 aagcgctgga tcgtgatggc ctcttcgatt tgaacgccgt cctgagcaga ccccttgtca
     4321 cctccgaacc ggttccagct gccccttccg ctgcagaaat gtttgcggcc ccggtgtcgc
     4381 agctgactcc gcagctccaa gagcagatca cggccgtgct gacggacgcc aaccagatcg
     4441 tcccctatga ccgtgcatgg gtgtgccact acgatcccat cgcgggcaac gtcgaagtat
     4501 tggggatggc aggcgacctc aagggcgacc aaaaagatag caagaaagac atgaagcccg
     4561 gccatcgcct cacgctggat agctcggccg caggttgggc aatccggcac cgcaagccca
     4621 gggtggatca cgatttggcc tcgacccaag gccgctttct cgaccataaa cagcttttca
     4681 aggatcggtt tcaatcctcg ctggttttcc catttttcgt caggggccag gtcggcggaa
     4741 cgctcacctt ggcgtcgagg gaagccggac gctaccagcc tacggatgcg cggacactcg
     4801 aaccgacgat tctgaagctt gccgatctcc ttcaggcgcc catgccggcg gcgacacccc
     4861 cggcaccagc ggtcgaggcc ggcggagttc catccccgtc cgagacgcaa gccgcggtcc
     4921 ctctggaacc cattatccgg aagcaggaac gccaggcagc catcggcgaa ttcagtgcct
     4981 ttctggccac cgaagtccgt gagcctctcg gctcgattcg ttcgcagtta gaagaggtga
     5041 ccggagaagg cattctcgat ttcgatccgc agacgcgcgt ggagaatgcc atgcgcgatt
     5101 tgatccgcgt cgaagcaatc ctcaacgaaa ttctcgactt tgccaaaccg ctggagctca
     5161 accggcggct ggtgcggatc cccgaggtga tcgaaagcgc gctgaccgtg gtggcgaccg
     5221 atcttgaggt gacaaggatt cacgtcgcca aagaatatgc cccgatcctc gcgccggtcc
     5281 gcggcgatga agccaagctc cagcaggtgt tcttaagcat tttcaagaac gcgggggagg
     5341 cgatgacgcc gggcggaacg ctgacgattc atgtgaccca gcatcgagca ggccgtggcg
     5401 tcgaggatca gattctcatc aagaacgacg gggcgcccat cccacctgaa attgtcgaca
     5461 aggtctttga acccttcttt acgacgaaga aagcgggaac cggtctgggt ctcgccaccg
     5521 tgaaaaagat cgttgaagaa cacggaggct ccattgccat cgcgagcgcg cccggcgaag
     5581 gcacatcggt gaccatccgc ctcccgggtg tcagccgcgg tccagccttc cgacaccgtg
     5641 gacggggccg ccggcctcct cgccgcggta attagccatc cattccactg attttatttg
     5701 aattttcccg cgcgactggc ttcatgccgg aggccggtgc ggcagtatcg actcggaaga
     5761 ttgaaccttg acacagatag cctctaatgg ctatgatccc gcccacttga ttcggattta
     5821 tttacgatcg caattcacgg caattcttca gccaccttta ttcacaaagg agtacggtat
     5881 gaagctcgta ttcggcactg ttgcaacagt ctccggtgtc ctgtttgcgc tgtccatggc
     5941 ctccgccaat ccggcgctcc ttccaaagca tgagggctac ccgatgaaga atagcggaag
     6001 cccggtcact ggccagccga cggcgaacga tccaggtcag tctgatgctc gcggcgagtc
     6061 cacgctgctc aagtcggctg ccttcgatga taagcatgcc aagcaagagc tcaagaagac
     6121 cgataatgag cggatcactg caaatgaggg agccggtcgg cttccgaaag tccagggacc
     6181 tcagattcag attgcgccgc cggtcacatc agccaccaag atcaccggcg atcgtaagat
     6241 cgaatagttc gttctttgcc ggatctacaa acaaggggaa gccgtgcttc gcacggcttc
     6301 cccttgttat ttcttctcgt gaaccctctt cacttcgtag ggcgccgggg ccctcgttga
     6361 ctcaaccgaa acctccaggg cttcattgcc cacacgccgg cataatccac accgattcgc
     6421 ggatgggttc caatcacgcc gcgaggtatc ttctctccac gatcctcaaa ccagaggtgt
     6481 actccactgg tcagatcgat ccgattcaat gatcgatcaa tgtccagcgc ccggcagagg
     6541 cgacctggcc catcaatcaa caatccgtca atctcaagcg ccctgatcaa cacggcagcc
     6601 ggaaactcgg atcgttccgt caccacgttt aaacaatggt gcatgccgta aatgagatag
     6661 acataggaaa ttcccggcgg gccgaagagc acatccgtcc tggctgtccg tcccttcgac
     6721 gcatggcagg ctttgtcctg aggtccgatg taggcctcaa cctcaatgat ttttccggca
     6781 acgatcccct tcccattctc acgcacaagg tactttccca cgagtgagcg cgcgacggtc
     6841 aacgttgagc ggctgaagta ggtttgagga agtatggctc tcaattacgc ctctctcttt
     6901 aatatcctct taggagcccc ctcataataa ccatccggca agaccgcagg aagcgaggtc
     6961 gtctgaagca cactctttcc ggacatcgag gagatcgagc ggccgagaac gaagctggag
     7021 gtcattttca ggaggcgatt aaaaatacca tgcgaggccg cccatgacaa accgcggatt
     7081 ccccggcacg aaatggatat ccgtgactcc tccagccggt tcgcctggca gacgggattg
     7141 aaaagcaaag gtggcctgct cccattgcgt gtcaaagaga ttctggacga acaggaatgc
     7201 ctcgaggcgt ccataagaga gcttgaccgg aagttgatac cgttcggtca agtcaaagtc
     7261 gatccacgat ggcgcgttga tcgtccggtc ctcggtcaag ggtctgactc ccaggtaagt
     7321 catttgcagt tgcgaggaaa gcccctctgg ccatcgcagg agtagtgcgg cataggcggt
     7381 taactcaggc gcgagaggaa tggccaagcc agtatctttg aattcggcat gggtccaggt
     7441 gaagctgccg ttaaagtaca gcggtccata aatctgccct cgtgcacccg cctccgcgcc
     7501 ataccgtctc gtgggcccgc gggcttcggt ggttccggca tctcccacaa atacgagttc
     7561 ggatttcaaa tccagccccc agaaggttgc ggtcaattcc attcgatctt gtccccaggg
     7621 tctggaccga aggccgactt ctatgccctg cgccctcgcc agaggagagc tgtcaccggc
     7681 aatcgtcgat cgcgcatcgt tgctgtgaaa gccagtgcca tagttcacaa aaaactccgt
     7741 gttgaaccac ggtccgagaa tcagattgcc tttcggactg atttggccag aatcttttct
     7801 tccggccggc tgttcaggac aggtatcgca gagattacgg acatcgaagg tgaagtagtc
     7861 ggcgcggaca ccgccggcca gccgcatcca cggcgtcggc tgcaattcaa gcttcatata
     7921 aggtgagtaa gacgcttcct gcacagcgtt ttgtacgatg gtgccggtcg acgtacgctt
     7981 cgtctgcgtt ccaaggcgga tatcggaaat gctgtcgttg cggacctgaa acccggcggt
     8041 cgcggtgctt tgaatcccga agatttcccc tgtctgccgg tatccaagat tcccgccata
     8101 catgtagcgt tgatcctgct gattgatgcc gtcaccattc accgggttat ccagaaagaa
     8161 tgtgaaattg gtccacaggt ccagcttgta gtacatccca taggcatcgg cgaagaactg
     8221 tccgccggag ggtatgtcat agtgatattg cgttcgcccg gtcgcacgct ccgtcttccc
     8281 tccttcggat ggatcaatcg acccgaatcg atcgatcgtg ccgtccgcga cggcccgaaa
     8341 gggaatctcc cctgaggcgt tccagttcgc cttgtggtag ctccctgtca cgctgagttc
     8401 cgagcgaacc gtcgggttca cggtggcctt gcccatcaga ttgaagcgaa agtaccgatt
     8461 gtcattctcg aacggcccgt tcgtgtaata gccctccgca gcaatcaacg tccgcacgtt
     8521 gtccctagtc ggagacagca tcaacagata ccgctgtgtg tcgaattggc caccggctcc
     8581 ttgcaccacg ttttcctgca acagctcacg ggtcttgaat gtcactgcgc cggcggtagc
     8641 aaagtcgcca aactcggcat ggtaggtccc cttcaccacg tcgatgccct ggatggtctc
     8701 cggaatgatg aaattgaggt cggtgtaccc ctggccatgc ccgtgggtgc gcaaattaat
     8761 cggcatgttg tcgataaaga acgcgacatc tgttccgtga tcggcgtcga accctctcag
     8821 gaaatactga tccgccttgc cggcaccgcc ggaatgttct accgcgatga accctggtat
     8881 caaacgcagg acctgagctg ggcggccttg gggctggaga agaatttcct tatccgggat
     8941 gaactgttgg gaagaggcgg ccaccgggcg ttcaccgacg accgatactt cagggacttc
     9001 gatgtccgga gcatccggat cgtgggctac cgataaggaa ggactatgaa ggatccacca
     9061 tagaagaatg gcagcgcgaa caatgcggga cataccagga ctcagctgag tcccttgcct
     9121 gccgtcgtca tcgtcagctt gccgtgcttc acgcctttga cgctgactaa cgcgtcggcg
     9181 acgtgcctga tctgtgagac ctttcctttg accaccagca cttcaaggca atggtcatga
     9241 tcaagatgca catgcatccc ggccatgatg tgtccttgaa aattgtgctg gatgtgcgtg
     9301 agtttcctgg tcagatcgcg cacgtgatgg tcatacacaa acgtgatcgt cgccacggct
     9361 tccccgtcac catcgctact ccattcctgg ctcaccagct gatcgcgaat gaggtcgcga
     9421 agcgcctctg agcgagtcgc atattttcga cgccgcacga ctttatcgaa gtcgtcgagg
     9481 aggtggtggt ctaacgagac gccgaaccga acaagtttct tcatcttatt gatgctacga
     9541 ttattaaatt cgtgctacga actatagcga tgcggcacgc gcgagtcaac acgcgcatga
     9601 cccctgggga cagcacaagg aaagacctga gagggcttgt ttactcgccg cgcaccggta
     9661 tgtagagcac ggtgccctgc cggttaacga gcaagagcgc gagatcggtg ggtttcatgg
     9721 ggtcggcaat ccgctggaat gttgaaaagt tcgggacggg ctgacggttg agttccaaaa
     9781 tgacgtcgcc tggttgaaga ccggaggatt cggccaaact gccttcttcg atgtcggtga
     9841 cgaccacgcc gctgttcacc gccaaatcca tttgacgtgc caacggtggc gtgacatcgt
     9901 cgaacacaac accagccaga ggatgagagg tcgatactgc ggctgctgcc tgactctttt
     9961 tggcccgctc acgcggagct tcctggacca tcagctcgac ctgaatggtt ttgccatccc
    10021 gcacgacatc gagccgatgt ttgctgccga tagcggacgc ggcgatcaaa ttacgaaggt
    10081 gcccgctgtc catcacgtcg cgtccgtcga accgctcgac gacatcgccc cgtttcaggc
    10141 cggctttttc cgcagacccc ttcgcctgca ggtcggtcac gatggcaccc ttcacatcgg
    10201 gcagccggaa gatttttccc aatagcggcg tgacatcctg agtagaagcg cccagaaacc
    10261 cccgcacgac tcggccggtc ttgatgaggc tttgcatcgc cgcgcgtgcc atgttgctgg
    10321 gaatcgcaaa gcccacaccg acgctccccc ctgtggggct cgcaatcgcc gtattgatgc
    10381 cgacgagttc tccgttgata ttcaccagcg cacccccgga gttgccggga ttgattggcg
    10441 catccgtctg aatgaaatct tcgaaatccg ccacgccgac gtccgcgcgc ccgaccgcgc
    10501 tcacgatccc gaacgtgact gttcggctca atccgagcgg gtttccgatg gccaggacga
    10561 aatcgccgac ggccagattg ctggaatctc cccaggcaac cgccggcaga ttcgtgcctt
    10621 gaatcttcac gaccgccacg tcggtcttgg gatcggtcgc aacgactttc cccttgtact
    10681 gccggcgatc ggcgagaatc acttccacgt cgacggcatc agccacgacg tgattattcg
    10741 tgatgatgta cccgtcaggc gagacgatga ccccggaccc ctgcccatac tggcgacgcg
    10801 ccggtggttc tttgaagaga ccgaacggaa gggtttcgtc gctgaatgct tggtcacgca
    10861 ccatcaccgt ggaggcgatg ctcacgactg ccgggattac cttcgatgcc gtggctttga
    10921 cttgattttg aagatcacct ccacctgaaa cagaaatcag gcgaccggat gcccatgccc
    10981 caccagccat tgacaacgtc aagaggcaca gtcctgcaca caaaatacga cgaaccacct
    11041 ggagatcctt tcgagaacag accaaatgtg ccgggtcaga aacttctgta gcctagcatg
    11101 cgtgcaaaaa gggcgcactc ataattctac atgccgcaag cgaagcgcat ttgaaatcac
    11161 cgatagagaa ctaaacgtca tggcagcact cgcaatcatc gggtttagga gcaatcccca
    11221 cagcggatag agcactccgg ccgcaatcgg cacgcccacc atattataga caaaagcgaa
    11281 gaacaggttt tgccggatgt tgcgcatcgt ggcctgactc aggcggcgag cccgaacaag
    11341 cgcccgcaga tcgcccttga ccagggtgac ccccgcactc tccatcgcaa tgtcggtccc
    11401 ggttcccatg gcaataccca catcggcaag cgccagagcc ggtgcatcat tgatgccatc
    11461 gccggccatc gcgacgacgc gaccttgcga cttcaagtgc gaaatgatcc gtcccttttg
    11521 gtccggcatc accgacgcat gtacctcgga aatccccaac cgcctggcga ccgcttcagc
    11581 cgtgtgcaca tgatcgcccg tcaccatcac cagtcgaatc ccttgggcgc ctaatattcg
    11641 gacggcctcc ggagtcgatg gcttgatggg atccgccact cccagaaggc cggcagcttt
    11701 gccgtcaatg gccacgaaga ccgtcgtttg gccttcctgt ctcaatcgcg tcgcctcttc
    11761 ctcaagcgcg gctaaccctt ggtcggtcac tgccgcttgt tctctgagca atgtgggcgt
    11821 gccaacggca acctggtgct gctctacaac gccggacact ccgtagcctg tcaccgaacg
    11881 aaactcttgg accggactca gcagcaggcg gcgttcttcc gcagaggcca cgacggcgga
    11941 agcgaccgga tgttcgctct tgcgttccac gctgccggca aaacggagca catcggcctc
    12001 gttccaggga ggcacgaatc gaatcgcgac aagcctcggc ttaccttcgg tcaaggtgcc
    12061 ggtcttgtca aacacgagag tgtccacctt cccgagcacc tcgagcgcct cggccttccg
    12121 taccaaaact cccgccatgg ccccacgccc agtccccacc atgatcgaca tgggtgtcgc
    12181 gagccccagc gcacagggac aggcaataat caagaccgcc accgcattga ccagcgcata
    12241 ggccagcctc ggttcaggcc cccagaccgc ccaggcgacc gctgtgacaa aagacgccgc
    12301 cacaacaagc ggaacgaagt agccggctgc cacgtcggcg acacgttgaa tcggagcacg
    12361 actccgttgc gcttcactga ccatctgaac gatgcgagcg agcaacgtct ctcgacccac
    12421 tcgctgcgcc tgcatgagta tgctgccggt cccgttgatg gtaccgccgg tcaccgcggc
    12481 gccgatcgct ttttccaccg gcaaggattc tcccgtgacc atcgattcat cgacggatgt
    12541 cgcgccctcg acgaccacgc catcaaccgg aacccgctca ccgggacgaa caaaaaggcg
    12601 gtctccgacg cgcacctgtt cgattggcac atcgacctcc ctgccatccg actccaaccg
    12661 cttggcggtt ttcggagcca accggagcaa ggccttcata gccgaggtag tttgactccg
    12721 cgcgcgcaac tccaagactt gtcccagtaa gaccagcacc gtaatcacgg ccgccgcttc
    12781 aaagtagacg gcgatcgagc cgtcatgttg gcgaaacgaa gccggcagga gatccggaac
    12841 gctcgtggcc acggcgctgt aaagataggc agcgccagtc ccgatcccta tcagggtaaa
    12901 catgttgggc gctctgttca gaatcgactt ccaggcccgc tcgaaaaatg gccatccgcc
    12961 ccacaagaca accggcgtcg ccagcagcca ttgaatccag ttcaggcgcg agccgccaag
    13021 gaggtccttc aggtgttgcc ctggaatcat gtcggacatc gcgagggcca tcacgaggag
    13081 ggcgggaccg aggctgaccc aaaaacgtct cgccatatcg tcgagctcgc tgttgggact
    13141 gtcctccagc gacacgactt tcggctcgag cgccatcccg cagatccgac aagcgcctgg
    13201 cttcgattcc aggatctcgg gatccattgg gcagatgtac tcgatcgccg caccgggtgg
    13261 aacggtcaca ggctcgggaa gtcgctgggc cggcggagtc aggtaacggg tcggatcctt
    13321 ccgaaatttc tccaaacagc tttgcgagca aaaatagtac gtcttgccgg cgtgcacgtg
    13381 agagccgaca gccgatgttg gttggaccgt cattccgcag acaggatcga cgtccccggc
    13441 tccgccaatg accggagccg gcatcatcat gggaagcgat ttcttggatc ctagtgtgat
    13501 gaggccggct gaggccggtc gtaacgcccc ttcaggatca gctttgaacg tttcgagaca
    13561 ggacagtgcg cagaaatagt acgtcgcccc tttgtactca tagcgacccg cagccgtcgc
    13621 gggatcgacg gtcattccac agacgggatc gatcgccatc gctcctcact cctcaccggt
    13681 tacttcttgc tgtccgactc cagaatgctg cggatgacgg tgatgatatt ttccgtcttc
    13741 atgttatcgc cctcgatctt gatatttccg tccaataaca acgttggagt atattgcacc
    13801 ttgattcttt cgccccactg tcgtccctca tcaaaagcct tggcgggttt tccggtggcc
    13861 atcccttctt caaacgcttt gggatcgagc ccgacttcct tgatcagcgc ctcgcgcaat
    13921 gagcgatcca acaccccggt catcttatcc tggtgaatcg tccggaagag gaccttcttc
    13981 atctcgtttc ccttccccat catcttcgcc tgctcataca tgtcgaacgg agtcgggagt
    14041 ttgttacgga atacgggaaa gcccaccatg gtcgcttcga gtttgttgcc gaactctttc
    14101 tgaatcagcg gaagaccggc ttcctcaaaa tgatggcagt gcggacaata aaaatctgcg
    14161 aactcgacga gcttcacttt gcccggcgag tgagtagact tttcttcttt caaaatctca
    14221 aacttaccct tcagctccgg ggaagcggca tgaaccggca ccattcccat gaacagcccg
    14281 accaagagca tccatgatcc gatcgccatc cccttcatta acacactgtt ctccatgacg
    14341 gctcctttcc tcaggtcaat cgcaccgaac tcattatatc gaacactaaa tcaacctgcg
    14401 ctttgttata atcaggccat gcacctacgg ccgtttcttg tttcctttgt cctcctgttg
    14461 gtattcacgg gctgtgcgtc gacgaaagag gcgggagacg gctcagcggc gaaccagatc
    14521 acgtttcctc aggtcaagga agcactggac tcgttcaaag gacaaacggt catgttcgga
    14581 ggccaggtgc tcaatgcgcg acgcctgaag gaagggactc ggattgaaat ccttcagctt
    14641 ccactcgacc ggtccggcca ccccggctat gatctcacgc aatcgcaagg ccggttcgtc
    14701 gccctgcacc gcgactttct tgatcccgcc acacttccat acggcaccct cgtgaccgtg
    14761 accggtcagg ttgcgggatc aattacgctc ccgctggacg aaacggaata tacccacccc
    14821 attatcgaca tcacacagct ccgcgtgtgg acgcatgtcc aagatttcgc gccctgcatg
    14881 cgcccctgcg cctggccagg tccgtactgg ggaccctact ggggtcccta ttggggtccc
    14941 tggccatacc gctggtaagc cggagattcg aagacaggct caggttaaag ctgaggtcgg
    15001 gcgatgcgta ctcacgtcaa cccttcgcct cagccttacc ctgccgaaat tatcgtccgc
    15061 ttgagccgat gatccgttcc acacgatcgt cacgcccacc caggttcctg tacttgtgat
    15121 actgggccaa tgcgcgcggc atgtccttac ggtgcagttc atagaagacg ccgagattat
    15181 agtgcgcctc ggcataatcg ggcttcaact ccacagcttt ctcgtattcg tgctcggcat
    15241 cctccagttt attcatgctg gtgtagatga cacccagatt catgtgtgcc tcggcatatt
    15301 caggccgata acggatcact tccagaaact ccttctccgc ttcaacaggt ttgttttgac
    15361 tccgcaggat aaacccgagg ttgtagtggg catcgacgag agccggcttg gctgcgatcg
    15421 cctgtcggta ggcgtaggcc gcctcgatca gatcgttgtt ccgctccgct agacgaccga
    15481 cctgatacca ggcatcggcg tgtttgggat tcgccttggt caaccgtccc agcatctcac
    15541 gagccgcttt gttgtcgccc tgcctctcgt acagcatggc caacccgaag agcgcgtcag
    15601 ggtgctccgg tcgaatcgcc aacagactct tataggtctt catcgatgcg gacacatcat
    15661 ggcggctctc gtagagtttc gccagatcct catacgaatc ctcgtggtga ggatcgaggt
    15721 cgatcgcgcg tttgagagcc tgttccgctt ccgcggtttt accctgagcc agatacactt
    15781 ccgccagatc gttcagcgca tccggatagg ccggcttcag cttgagcgcg tgtgtgaagg
    15841 catcgacagc ctgagccggt ttcttctctt tgaaatagac gagaccaagc aaatggtgcg
    15901 cttcagccga ggagggattc aactcgatgg cttttctggc agcctcggat gcgccagcga
    15961 gatcgcccga cttatataga tcagcagccc tttcaatggc tcgctttccc gtatcggcag
    16021 gtggagtagc ctggataggg gcgatgggaa gcagccccag ccagactccg atcacgagat
    16081 ggaagtatcg tcgagcgagt gccttcaaca gcgcgctccc agagaaagag aaggatgatc
    16141 caactatacg gtgggcaaga aacgaaatca acgcgaggaa gaagtgtctg aattgcgtat
    16201 gattgcaggg ggagtcttcg tgggaaaact cccccgtcac aacctcttcg gaacgtactg
    16261 aacagcccga ctgtcaacaa ccaacccggt gataattgcc tatccccctg ctggtttcat
    16321 caactgaaac gatttcgtca atccaaaaac ataactggtc ccctgtgcca aattgtcatt
    16381 ggaatcccgc gcgaacggaa tttgtgcaaa gaaatacaat gacgtcatat tcgtccactt
    16441 ggattcaatg agctggccca gattcacttg aaaaccgggc gaaaaggcat ggtaggtgga
    16501 tccggtggtt ggcactgcac gattcttgat tgttgggtca agcacaaccg gttcacccgg
    16561 aaagtccggc gcgtcggctg gagtcgctga gcgggacaac gatgaactga aattgtcatg
    16621 cacgagatac cgatagttca cctggccggt aagaatcagc cagggtgttg tcacgagatt
    16681 aaagccggca ttgagcatgt attcatcacc aaattgatac ccatagttgt tccggaacgt
    16741 atgccggtaa ctggccgaag cgaattgatt gagccgatgc gggatcaatt cgtaggtctg
    16801 ataaatcgtc gggttcaacc cgacttggcc ccgcccgagt tgtgtgctgg actccatcag
    16861 ttcccctgcg ctgtcatgcg cgtgggtatt accagtcgga agatagacgc cgaaccccag
    16921 caccaccatg ctgcgaagag tgggtaagac gttgtacttg aggccgactc gcatatcccc
    16981 gatcccatcg gaggagaact gtgtcggcgc tccttccccg gccacaccat cctctccgag
    17041 gccgtcaata tgcttgtgcg tacgccacat gtaggggagc gtgatttgaa gtccgaaccg
    17101 gtccgtcaca ccgtaattaa tgtcaagcgt ggcttgttga gtaatggtcc gcgtttcctg
    17161 gtgatgattc agaatcattt gccggctccc ttgatcgacc gccggaatca caccggaggt
    17221 tccatcgagt agtcgcatgg gcgtgtaatt gtaaatgcca ttgaccgtga gcaggccttc
    17281 ttgcggaacc tgctgttggg aaccgatcac aacaaagcag gttaccgccc cacacgaggc
    17341 gccgactgga gaaacaaatg ccatcagacc ggtaatccag gcaaaagtaa ctatcggaat
    17401 caatttattt aataaattca tttcagtcct atctttctct catcatcccg tgcatagagc
    17461 acagaactgg tacgtggtca ttgagtgttc ccgaacctgg ctacagagtc ggcccagttg
    17521 ctgatcggat ggttatcagt tcaggatcga ttcatacagc caggcaaagc tagcttgtca
    17581 tgaagcagag gaagcgggag gaccacggag gaaggtaaag gaggagaagg aggcggcata
    17641 ttggtcgacg tgaaaagaca ggacccggtc gaccaaacgg agtgtcgatt ccaggctgac
    17701 agaagaagtt tcaatcccct ggccggcggc acagagccaa gcacaaagtc cgctgctgtg
    17761 tgttccggct tgatggctcc catgcttggc cgcatgggcg acgctcggcg cggccatgaa
    17821 cccgttcacc agaacgatac acagcagcag ggaccagacc agtccggatc gtttcaacgc
    17881 gaccataaca atcttctacc gcagcgaggc ggccttcacc ggcgcttgtt tctggatgcg
    17941 caccacgacg tcgtaaatct tctgttcggt caggagtccg cctttgatgt actcctgtac
    18001 gataccctgt tcatcgatga acacgctgac cggcaacccg aataccccga actgattggc
    18061 gactttgttg tcgcgatcca tcagaaccgg gaacgtgtgc ccatattgct ggatgtgctc
    18121 gcgaactttc gcatcgtttt ccaactcatt gacggccagc acgacaaacc ccttgtctcg
    18181 cagcttgtcg tacgtcgtct gcatcgcagg catttccgtc gtgcagggtt tgcaccatgt
    18241 tgcccaaaag ttcagaagga cgatcttgcc tcgatgctgg ctgagactct gcgtcttccc
    18301 gctcagatcc acgagttgaa aatcctccgc cggcgttccg accaccggca cgcgcgagcc
    18361 catgctccag gccactccag tcgcgagtgc caccagcgac gccgcgagca cgaggctcag
    18421 cctgatgttg ctcacatatc ttcttaggaa ggtgttcatc gtcagcctct ccatccaaaa
    18481 aatgataaac cccgggatgc tcgactcagc tattttgctt gaagcgacgg aactccccct
    18541 tttcgggaat ggagaccgca cgcgagagtc gaatcagata aaggctatcg aacaggagga
    18601 gcgcgggtgg cgaggatacc ggagaaaatc gttggaacct gatcggtgga ggtgaactcg
    18661 acgaagtcgt gaggcatcag tgcgacagga acgaactgga ctgctgcttc accagcttgt
    18721 cccgcggcgc acatccagga gcagagcacc gtgccgtgcg tggctttttg atggtgcgcg
    18781 tggtgggatt cgtgggtgat cgactgggcg gaggccaatc cgccgatcac gaggacgcag
    18841 agcaccaaga gacaggcgag tgaacgttga aggaagctgt gcgtcatact cccacactct
    18901 aacaggaccg gcgggccgat ggtaccggag ggcaagggac agagtcaagg caaccagaga
    18961 ggtgaaacct ttgtcttcca gataggattc gcactacgtt tccggtatac tgccgtcata
    19021 tctttctgtg gtatgggtgt cactactctg ccctttcctt aggaacaagg catgctcaca
    19081 caactcctga atctggtctt cggtagcaag aacgaccgtg aaatcaaggc tttgatgccg
    19141 atcgtccagc gcattaacag cctggagtcc ggcctgactc ccctgtccga ccaggcctta
    19201 gccgacaaga cccaagagtt caagaagcgg ctgtcggccg gtgaaaccct cgacgatatc
    19261 ctgcccgagg cctttgccgt ctgccgggaa atgtccaggc gcaagctcaa catgcgtcat
    19321 ttcgatgtgc agctgatcgg cgggatgatc ttgcataaag gccgcattgc cgaaatgaaa
    19381 accggcgaag ggaaaacgct ggtcgcgacc cttccgattt atctcaacgc cctcgaaggg
    19441 aaaggcgcgc atctggtcac ggtcaacgac tacctggcca aacgcgacgc gcaatggatg
    19501 ggccagctct atcatgccct gggcctctcc accggcatca tccagcacga cgcgtccttc
    19561 atcttcgatc cgacctacga cgcatctgac cgacgcttac agaatctccg cccctgcacg
    19621 aggcccgaag cctatcgcgc cgatattacc tatgggacga acaacgaata cgggtttgat
    19681 tatctccgcg acaatctggt cgtcacggat ttgaatcagt gcgtgcagcg cgatctgaac
    19741 ttcgccattg tcgacgaagt cgacagcatc ctcattgacg aagccaggac gccgctgatc
    19801 atttccggcc cgacggatca aacgaccgac ctctactacc ggatcaacgc gatcatcccg
    19861 cagctcaagg ccgagcagga ctacacgatc gaagagaaga ccaagacggc ctcgctgacg
    19921 gaagacggta acgtccgtgt ggaaaagctg ctcggcgtcg acaacctgta cgatccggcc
    19981 aacatggatc tcgtccacca cgtggtgaag gcgttgcaag cctacgccct ctacaagcgc
    20041 gatgtggact acgtcgtgaa agacggcgag gtgatcattg tcgacgaatt caccggccgg
    20101 ctgatgccgg gtcgccgctg gagcgacggc ctccaccagg ccgtcgaggc gaaagagggc
    20161 gtgaagatcg ccaacgagaa tcagacgctg gcctcggtga ccttccagaa ttacttccgc
    20221 atgtacaaga agctcggcgg catgaccggc acggccgata cggaagccgc ggagtttgcc
    20281 aagatctata atctcgacgt caacgtggtc ccgaccaacc gcaagatgat ccggctcgac
    20341 tatgccgacg tggtctatcg cacggagaag gaaaaatttg cggccatcgc cgaagaaatc
    20401 aaagagtgcc acgaacgagg ccagccggtg ctcgtcggca cgatctccat tgagaagtcc
    20461 gaaaagcttg ccgggatact gagccgaaac ggcatcaaac acaacgtgct caacgccaaa
    20521 cagcacgagc gtgaagccga aattgtcgcg caggccggcc gcaaaggcgc ggtgaccatc
    20581 gcgacgaaca tggccggccg cggcaccgat attctgctgg gtggcaatcc cgattttatg
    20641 tacaagcagg tcttgtatcg ggaagagaac cttccggact cccgcaagct cgaagtcttc
    20701 gaggagatcc gctccgactg tgaaaaaaac aaacaggagg tcatcgccca cggcgggctt
    20761 cacatcctgg gcaccgaacg gcacgaaagc cgccggatcg acaaccagct tcgcggccgc
    20821 gccggccgcc agggcgatcc gggcacgtcg cggttttatc tgtcgctcga agacgacctg
    20881 atgcggatct tcgcatccga gcgggtctcg caactgatgc tcaagctcgg gatggaagaa
    20941 ggcgtaccga tcgagcacgg catggtcacc cgcgcgattg ccaacgcgca aaagaaagtc
    21001 gaagcccaca actttgagat tcgtaaacag ttgctcgaat acgacgatgt catgaacaag
    21061 cagcgcgagg tcatctacca gcaccgccgc gccgtcctcg caggagccaa tctcaccgag
    21121 gacatctacg atatgatgac ggatctggtg gagtcatcgc tcaacatcta ctgccctgca
    21181 gagcaatatc cggaagaatg ggatatgaaa ggcctcacag agatgatgca cggccaattc
    21241 ggcgtcgaca tcacgcaagg caagcacgac ggaggggaat cccttcgcga catggggcgc
    21301 gatgcgctgc ttgaagatct gaaagcgcag gtccgcgacg cctatggacg aaaagaacaa
    21361 gagctcggct cggatctgat gcggttcctg gaaaagaact tcatgcttca ggtcatcgat
    21421 caccattgga aggaccatct cctcggcatg gatcatctac gcgacgggat aggacttcgc
    21481 gggtacggcc agaaagaccc gttgatcgaa tacaagcgcg aagggttcga gctgttcgcc
    21541 ggcatgatgg agcgcatcaa atccgacaca ctcgaccggc tgtttcgtgt ccaggcagtc
    21601 cggaacgagg gcgaagcagc cgctccgcca ccacctccga tcatttcaca tcctcaaccc
    21661 aagctcacgc tcaatcgcag cgacgagccg gtcgcggctc agacggtcca ccgtagcgac
    21721 gacaaagtcg gccgcaacga cccctgcccc tgcggtagcg ggaaaaaata taagaagtgc
    21781 cacggggcgt aggttaggct cgctgcgccc tcctctctcc cccgcttcaa ttcgaccgtt
    21841 ctagatctca cagctgtttg gcgtcgaaac ttgttccgtt ctatgcagcg catgaacgga
    21901 ccttcgaaca tcgacgccac tcgcgttcag cgagccgcag ctgttcgcca gaacggtgcc
    21961 cgaattgtcg cgaaggtcga gagatggcgc agcgagcccc ggttggggac gaagaaactg
    22021 aaaaggcccg cgcgtagcat cagcgacgtg agggagagaa tgtgcagtcc acagattgag
    22081 atttatgatg aacagcttgg ctaatcacta cagcggaccc gcttttacat cgactatcca
    22141 tcaagcaaga tttcgtctca tctcgtcata ctgctctttc gtgagttcac cgccggcgta
    22201 gcggcggtcc agaatctgtc gtggcgtttc gtgtcggtgg tctcccatca ttccacaccc
    22261 cggcctgaaa ccgcgccgcc agaaaaagcc catcatgcca atcatcacga ggaagaatag
    22321 aagtgggaaa atccacatcc atcccatccc agcaccaggt ccacaccatg cccacatgga
    22381 acacttctaa ctttctttat ggcagcgcct tactggcgct ttgttcattt agtttggaga
    22441 tgattttgtc ggcaacggac cgcgccacct ctttgcgttc ctcttctggc acgtcgtcca
    22501 ttgagaacac gtcagtatat tttttgtaca aagggagcgc gtcgatgtgg atcactttct
    22561 ccctatcaac gaggttgttg acaaccctgc caatgcattc taagaaacag ccatcaatca
    22621 caacgatctt ctccgcttcc ttgacccaac gcgccatagc cgaatgaggc actaaggcgg
    22681 cttctgcata acaacagcga gcgtagctcg gcatctccct agaaactaag tttgctgcaa
    22741 gccgtgcgat ctcaccgcgg atgcaaggac cctcacacga aaagattggt acctttttct
    22801 cgatcatgtt ctgccttgcc cacttttcac ctgctgggca gacaccttcc acttggtcaa
    22861 cagcaaccgc aaaatcggct gtggctttat ccgctgattt agtcatggcc aaggccccct
    22921 tcctcgaatt aacagagtag gacccgcaaa agacgaactg atgcgactaa tgtgccagtt
    22981 caagggagct ttctaaacca cagaaaacca atcgcttggg agtcagctgc taatgtttat
    23041 cgaatgattt gggcacgtac gtgaggcgta agggggcagg atttaggatt gttctggggt
    23101 attatgcctc tttgcgcgag accttttctt aggcgcctaa tcattgtccg gcgagagaaa
    23161 tcttggttca taaagttgtg gctaactaaa atgtggcaga actattgtgt acgatcggtt
    23221 tccatcggtt cctcccttgc ctctcacccc tctgtaagtg attgagactg aaacgattgg
    23281 tctggatcgg gactatcgtc tctgggactg tagggttttc aaatttcccc atccctccct
    23341 cttcaaatgg cgggatcgcg gagccatgtg agcgacttgc cagcaattcg ggtaccgttc
    23401 tgttgaaccg ctgcagctcg ctctggcgag tggcgccgat gtccgaagtt ccgtcccgcc
    23461 atgcgccgga cggaacgagt ttcggtggca agcagcggtg agacgtagaa cggtcgaatt
    23521 gccggcctgg agcgattgcg gcgctgcgat cccgccctat tcttccttat tccaccttga
    23581 cttagcgcgc tccccaaggc tactctacgc cacttttggc ccgccaacgg gcacaggtga
    23641 ggtattccct ccgtccttgg ccgcctgttc tatgttttgt cagcgacaaa gggaactgtt
    23701 catgacgctt gggcaccctg aactcgttgc cgccatcgct tccgagatca cctcctcggg
    23761 cccgattccc tttgtccggt ttatggaact cgctctctac catccgcagt atggctatta
    23821 catgcggccg ccggaatccg gcgccgaacg gatcggttgg tccggggatt tctataccag
    23881 ttcagacgtt cacccgattc ttggacaggc cctcgcgaag caagcggcac aaatcgatgc
    23941 caagctggga tctccggatc catttactgt cgtcgagatg ggtcccggta aaggcgtctt
    24001 gtctcgcgat ttcctgaaag ccattgacag tcagtacggt gaactgagcc ggcggctacg
    24061 atacgtgctg atcgaacgca gtccggccat gcgcaccttg cagcagcaac aactcgaacc
    24121 ctggttgaat caaccggggc gtcttacctg gcttgaagac ctgacgagcc tcgcaccggc
    24181 cagcgtgacc ggtctgctgt tcagcaacga attgccggat gccttccctg ttcatcgcat
    24241 tcagatggtg gggaaagagc cacaggaaat cttcgtggac tatcaagacg ggcgatttgt
    24301 ggaatgtctc aagacgctct cgcagccggc gcttcgaaac cattttcaac ggctccgcct
    24361 cgaattgccg gatggttatc gaactgagat caatctgcag gccctttcct ggatgcagct
    24421 ggtggcgcag agtctggctc gtggcgtcgt cattaccatc gattacggcc attctgcaca
    24481 agatctctat ggcccggatc gatccaaggg gaccttcctc tgttactact cgcagatggc
    24541 gtcggacgat ccctatgtcc gcatcggcca gcaagacatg acggcgcatg tcgatttcac
    24601 gagtctggcg acggtgggtg aagagggcgg tttatccgtg acggggttca ccaatcaaat
    24661 gagtttcctg atgggcctcg gcatcgaaga gacgatcagc aggctcgacc cggaaagccc
    24721 gcaatttcga gcagcgatcc acttgttaat gccggagggg atgggccgca cgttcaagat
    24781 tctggtccaa cataaaggca tcgagcggcc ggaactcgac ggattggcct tcaagccctt
    24841 tttcggctca gcgctgagcc tgcatgcggc ggcgtaacgc acaaggagcg tcatggggag
    24901 cgaagcggtt ccgctgaatt atctgccgat tctcctgttc atcggcatcg cgatcgtctt
    24961 cggggcgatc tcgctcctgg ccggctggtt cgtcaggccc agccgcccgt atcgcgccaa
    25021 gttgaatccg tacgagagcg gcagtccgct cttttccgat gcccgcatcc agttcccaat
    25081 gcggtactac atcatcgcga tgctctttgt cattttcgac atcgaagtga tctttatcat
    25141 tccctgggcg gtccgatttc aatcgctcgg cctgttgggc ctgatcgaga tgctggtgtt
    25201 catcgcaatc ttattggtgg ggctctggta tgcgtggaag aagggggcgt tagaatggga
    25261 ttaacagatc gtaaggcgtt aggcgtgaaa cgtgaggggt ctcccatttc accttacgcc
    25321 ttacgcctca cccttcacgc gaggcaacca tgagcttttt agaacggcag tttgaagcaa
    25381 acatcatcac gacgaatttg gatgcggtcg tgaactgggc acgcaagtcg gccttgtggc
    25441 cgatgacgtt cgggctggca tgctgcgcga tcgaaatgat cgccagcgta tcgtctcgct
    25501 acgacctcga ccgtttcggc gccggtgtgt tccgcgcctc accccggcag tccgatctta
    25561 tgatcgtggc aggcaccgtc tcccgcaaga tggctccggt gatccgccgc atctacgatc
    25621 agatgccgga accgcgctac gtgatttcga tgggatcctg tgcgacatca ggaaaccact
    25681 acaacagcta cgcggtggtg cagggcgtcg atcagatcgt cccggtcgat gtctatgtac
    25741 cgggctgccc gcctcggccc gaagcgttga tggacggctt gctgaagttg caggaaaaaa
    25801 tccagcgcga aaaggtgttt gtgaaatagc ccgcgaggcg agcgggagtg gcgggacgcg
    25861 cagggctggg acatcgcgct tttctcgcat gtcgcgcgcg tctcgcagaa tgacactatg
    25921 caacctctga tcgaaacgtt gatgaccagg tttcccaagg ccgtgatgtc cgtcgaggcg
    25981 gacactgccc gttccgaagt gtcgatcctc gtcgcagctg aacacctggt ggagctggcg
    26041 acgtacctcc acgatgcgcc agaagcgtcg ttcaaccatc tgaccgacat ttgttccgtc
    26101 gactacccgg aagaccggca gcgcttcgaa gtggtctatc acctccattc actcccccat
    26161 cgccgacgcc tgcgactgaa agcccgcttg cctgaagaca acccgacgat cgcatcggtc
    26221 accggcattt ggaagggtgc cgagttcctg gagcgcgaag tctatgacat gatgggcatt
    26281 acgttttccg gccacccgga tctacggcga atcttgatgc cggaggatta tgcagaaggc
    26341 taccctttgc gaaaggactt cccgacggaa ggccgtggct ggcgcagcca gttcgacttt
    26401 atcccccgtt tggacgaacc gccgggggaa gccgcaggga gcgaactccg cgaggaacag
    26461 aaacaggcat ttcgggctga accgtcaggt tcccgccgcc gcgaggaact tctcctcaac
    26521 atggggccac agcaccccag tacccacggc gtgctgcgcg tcgtcctcga actcgacggc
    26581 gaacgaatcg tgaaggcgac gcccgacctc gggtacctgc atcgaggtgt ggaaaaactt
    26641 tccgagggcc tggcctacat gcagatcatc ccccacacgg accggctcga ctacgtctgc
    26701 gcgatggcca acaactacgc ctatgtgcgc gcagtcgaaa agttgctggg catcacgatc
    26761 ccggaacggg ccgagtacat caggacgatc gtcgcggaga tgcagcggat tatcggccac
    26821 cttttctggc tggggaccca ggccttggac atcggcgcga tgacggtgtt cttttggaca
    26881 ttccgtgagc gcgaaatcct gctcgacatg ttcgagaaac tctgcggggc gcgactgacg
    26941 ctgaattact accgcatcgg gggtgtcgac agcgatttca cgcccgacct ggtcctgcgg
    27001 ctcaaaacat tcctcgacac cttccccgat aaggtgaagg agtacgactc gctgatcgct
    27061 tcgaatcgca tctggctcgg ccgcaccaag aacgtcgcgg tcatctcgcc cgaggatgcg
    27121 atcaatttcg gctgcaccgg accggttttg cgcggttcag gcgtggccta cgacatacgc
    27181 aaagaggaac cgtatggggc ctacgacaag gtcgactggg aagtgccggt cggcaagaac
    27241 ggagacacct acgatcgcta ttggattcgc atggaggaaa tgcggcaaag cgcccggatc
    27301 atcaaacaat gcctggacca gatgcctcag ggccccatca tcgcggatgc gccccagtac
    27361 attcctccgc cgaagcagct agtcatgcgc gacatggaaa gcctgattca ccatttcatt
    27421 attttcaccc agggcctgaa gccgccgaag gcagaaacct actgcgcaac cgaggccccg
    27481 aagggcgagt tgggattctt cattgtcagt gacggcagcg cccgtcccta tcgattgaag
    27541 atccgttcgc cctcgttcgt ccacatgggc gcattcgatc atatggcccg tggctatttg
    27601 atctcggaca tcatcacgat cttcggcaca tacgacatcg tgatggggga atgtgacaga
    27661 tgaagccgtg aggcgtgaaa cgtaaggtgt caggcgaggg aacaggtctt tcctctaaca
    27721 cctcacccct tacgccttac gaagggttct cgaagagggc tggatgctga aagaaaagta
    27781 tcagagagaa atcgacgaat tactgagccg ttatccggtg aagcggtcgg cattgattcc
    27841 gctgctgtat ctggcgcagc aggagaacgg ttatatcacc gaagcggcga tgattgagat
    27901 cgccggcctc ctgaagttga cgccgcctca ggtgtacgag accgcaacct tctatacgat
    27961 gttgaatctc aagaaggtgg gccggtttca cattcaggtg tgcaagtcgc tgatgtgcgc
    28021 gctggtggga tcagacaccg tcatcgattg gatcaagacc aagatcggca tcggcccggg
    28081 agagaccacc cctgacgggc tcttcacgct gacggcggtg gaatgtctcg ccgcctgcgg
    28141 caccggccct atgatgcaga tcaacgacga ctattacgaa cggctgacgg aagagaaagt
    28201 cgaccggatt ctggccgacc tcaagtcgac cggaaccagc tcactgaagt cgggcccgtt
    28261 tatgtggccg gaaccagtca atgtcgtgag gcgtgaaacg taaggtgtag agggcaggca
    28321 gtcctctgag cttcgcctaa cgcctcacgc ctgaccccta acgagaacaa atgccgaagc
    28381 acgaaccggt acttctcaag aatatgttgc agcctggtta tacgggctcg ctcgtcgact
    28441 acgaaaaagc gggcggctac caggcattgc ggaaggtcct gggcaagatt gccccgggcg
    28501 atgtcacgca gctggtcatc aagtcggggc tccggggccg cggaggcgcc ggcttcccga
    28561 ccggcgtgaa gtggggattc ctccccaaag attatcaggg cccccgctac ctctgctgta
    28621 acgcagacga gagcgaacca ggcacgttca aagaccgcca actcatcgag cgggatcccc
    28681 atcagatgct cgaaggcatt gtattggcct gctatgccat cggagcagaa tccgcctaca
    28741 tctatattcg cggcgaattc gtattgggcg cacaaattct agaacgagcc ctcggcgaag
    28801 cacgcgcgac aggctatatc ggtaagaaca ttctcgggac cggcgtcagc gtggacgtat
    28861 gggtccaccg cggagccggc gcctatattt gcggagaaga aacagccctg ctggaatcgc
    28921 tggaaggtaa acgcggactt cctcgtgtga agccgccttt tcccgctacg cacggcctct
    28981 ataacaagcc gacggtggtg aacaacgttg aaacgctcgc aaacctgccc cacattatca
    29041 gccggggccc cgaatggttc gccgcgatcg ggtctccgcc gaagagcacc gggacgcgca
    29101 tcttttgcgt gagcgggcac gtgaagcggc cggggaatta tgaagtgccg atgggcattc
    29161 ccttccggga attgatttat gagcatgccg gcggtatgcg gggggagaag ccgttaaagg
    29221 ccttcattcc cggcggcgcc tcggcaccgt tcctgacgcc ggatcatctg gacgtcaagt
    29281 tggatttcga atccgttgcg gcggccggat cgatgctggg ttcgggaggc gtgacggtga
    29341 tggaagaagg cacgagcatg gtctgggccg cgctgcgtct gatggaattt ttctatcacg
    29401 agtcctgcgg gaaatgcagc ccttgccggg aaggcagttc ctggctggtc cagacgatgc
    29461 gccgcatcat ggccaagcga ggccaggcgc aagaccttga aacgctgacc gatctgtgca
    29521 agaacatcgc cggccgcacg gtctgtgcct tcggcgatgc cgaagtcgcg ccgatcatga
    29581 gcacgatgaa gcactggcgg cacgaataca ttgcactgat tcatgaggcg gaagcagcca
    29641 atctgctccg tccggaaccg gcgttgaagc gacattgagt atgacggaca gcacaaccga
    29701 cacggtacgt ttgaccatcg acggcattcc gatcacggtg ccaaaaggca cgctcgtgat
    29761 cgaggcggcc cgacgcgtcg gcgtcatgat cccgcatttt tgttaccacc cgaagctgaa
    29821 accggacgcc aactgccgca tgtgcctggt cgaggtcgag aagatgccga agctccagac
    29881 ctcttgcagc accgtagcaa cggaaggcat ggcggttcga accgcgacga cggtggtgaa
    29941 cgacgcccac aagtcggtac ttgagttcat tctggccaac catccgttgg attgccccgt
    30001 ctgcgatcaa ggtggccgct gcgacctgca ggatttttcg catcaataca cagcgacggc
    30061 gagccgcttc atagaaacga agcgcatatt tcagaaagag tacttcagtc ccctcatcga
    30121 aacgcagatg aaccgctgcg tgcagtgcct gcgctgcgtc cgctactgcg acgaagtgat
    30181 ggacgtgaag gccctggcac cggccgggcg cggcacgatg acggagatta aatcattcgg
    30241 cacacaccag ctgaattgcg agttctgcgg cggctgcgtg cagatctgcc cagtcggtgc
    30301 catcaccagc cggctctcca tgtacgagta ccgcccctgg atgctcaaac gcgccgacac
    30361 gatctgccag tattgcggcg acggctgcca gatcacggtc caaacgaagg ataacgagct
    30421 gatcgaagtc atgtccgctc agggagccgg acggaacaac ggggacctgt gcgcaagggg
    30481 attcttcgga taccacgccg ccgcccatcc ggatcgcttg accaagccac tcatgaggcg
    30541 caacggcgct ctcgttgaaa cgacctggga agacgcattg gaatacacgg cggagcagat
    30601 ctccaggctc aagaccagcc atggcgccca ggctttcggt ggattgatct ccagccgctg
    30661 cacgaacgaa gagctgtacc tgttccaaaa agtcatgcgc accgcgatcg ggacgaacaa
    30721 cctcgacagc agcgcgcgtt acggacagct caacgcggtg caggcgatgc gccgcataca
    30781 ggggactcat cgctggaccg tcacgttcga ggacatcatc caagccgatg tcctcttatt
    30841 ggtaggcacc aacatcaccg aagccaatcc cattgccgga ctcaaggtca aagaggccgt
    30901 caagaaacat caggcgcgcc tcttcaccat tgaatcgatc cagccggccg tcgacacgat
    30961 cagcaatatc accaacctgg ccgagctgca tatctgtgtc ccgccggcac aatatgatcg
    31021 tgccatcttg ggattgctca aagctgccgt cgaaggccag caggttgatc agacagtcca
    31081 caatccctcg tccgaatttg tgacggcagt ctcgcaagcg cttgcgcggt tgtcttggcc
    31141 ggatatcgag gccggaacag gaaccactcg tgagaccttc gttcagctgg ctgccgggat
    31201 cgccaagggc aggcgcgtcg tcgtgctcgc cggccaagac ctgttgcgaa gcgccggcgg
    31261 ttatggcatc tgcctcaacc tcctcgacct cctgctgctc acgggaaaat tgacagagtc
    31321 cggctgtggc ttcgccccct tggcggaaga aaataacgat caaggcgccg tggaaatggg
    31381 ggccatagcg gaactccttc cgggcgtctt ggatgtcgcg aaccaggaag cccgagcgca
    31441 gatgggccgc gcctggaaaa ccgaccttcc tcttgcagca ggcgccacgc tcgtcgagat
    31501 gattgatcgt gcccattcag gtctcctgaa ggggatgctt gtggtcggag aaaacccggt
    31561 tggaagcttg ccgcctcacc tggaagtcgg cgaggctctt cggaagctgg aattcttggt
    31621 ctgccaggag ttattcctca ccgagaccgc ctccctggcc cacgtcgtcc tgcctgcttc
    31681 ctcttcgttg gagaaggccg ggacctttac caatgaagaa ggccatgttc aagccgtgcg
    31741 ccccacgatc gaaccggtcg gagaaagccg ccctgattgg gaaatcctct cagccatttc
    31801 gatgctctta ggtgctccgc gcgattatgg ggatgcgaag gaactcctca ctgagatccg
    31861 cagcgtggtc cccggatacg gattgctcgg cccgggaccg attcctccga aagtcgatcg
    31921 agctgcgatc gagcgttatg tcacaggggg gtatcgccag gacctcgccg gtcgatatac
    31981 tctggccact cacgccatgc ccctagaggg aacgatggag ttgcgattgg tccagagcct
    32041 gttccattcg ggaaagttct cgacccgttc gaaaggcttg cttcagatcg aagcgcgggc
    32101 ttcgttgcga atgaatcccg tggatgcggc gcgtttaacg ctcggcgacg gtgatcgcgt
    32161 ccggctctcc aatcagcgcg gcgacctgaa ggcgaccgtg aagctgctgg aacgggtgcc
    32221 gcaaggggcg ctctggtttc ccgatcactt cgcgcaggag gcggccaacc tcttcgagtg
    32281 tgcgatcgat cctcacacga aagtgccgtc gattcgaaca gcggcggtgt ccatcgtcaa
    32341 gatcacatga gccaatggct atacatccac caatcacttg ttatccggga gcgttgtcgt
    32401 gactgaacta agcttgagac ttgcagtgtc actcgcccag attgcagcgg tcatgggcat
    32461 cgtgatgttg acggtcatga tcctgaccct cgccgaacgg aaggtcctcg gctggatgca
    32521 ggaccgcatg ggcccgatgg aagtcggtcc ctacggcatt ctgcagccaa tcgctgatgg
    32581 cttgaaattg tttttcaagg aagacatcgt cccgtccggg gcgaataagt tcatgttcag
    32641 cctcgcgccg atcctcgcga tggtcccggc cctgatcgga tttgccgtca ttccattcgg
    32701 accgagtcac aagttcgaaa tcttcggcat cacggtcgaa ccgttcgtga tcagcgacat
    32761 taatatcggc atcctctaca tcctggcctt cgcctccatc ggtgcctacg gcatcatcct
    32821 cggcggctgg gcttcgaaca gcaagtactc cctgttgggc ggcctgcgtt cagcagctca
    32881 agttatcagc tacgaattga acgtcggcct ggcgattgtc ggcgtgctga tcctcgccgg
    32941 ctcgctcagc ctcgtgaaga tcaccgaggc ccaatccggc ggattctggc attggtacgt
    33001 ctttgcatta ccggcccctc aaatctttgc ctttgttgtc tatgtgatct ccgccgtcgc
    33061 cgaaaccaac cgtgtcccgt tcgatttgcc ggaagcggaa agcgaattgg tcgccggctt
    33121 ctttacggaa tatagcggca tgcgcttcgc gttcttcttc atcgccgaat acgccaacat
    33181 ggtcctggtt tcctgcgtgg ccgctgcgat gtttctgggc ggttggaacg ccccttatcc
    33241 ggggactatt ctgggacata tcgggctgga gcaactcgcc tgggtcgaga acatcgcctg
    33301 gttcacggtg aaggcttatt tcttcctgtt cctgtttttc tggctgcgcg ccacgttgcc
    33361 ccgtcttcga tacgatcaac tgatgaagtt cggctggaag gtcatgttgc ccatcgcctt
    33421 gggcaacatc gtcgtgacgt cgatcgccgt ctacttattc cccaggtaac ctaggacagg
    33481 gagcgacgaa agattggtta tgcagcgctt cacagcatgg ctgaaaacaa tcgtcttcta
    33541 cgaattgctc gtcggaatga aggcgacgat gtcccatttg ctccggtaca ggcccattac
    33601 gctgcagtac ccccatgaga agcggatgct gccggataat tatcgcggga tgttggccct
    33661 gctccgctac gatgacggaa cggaaaaatg cgtcggctgc gacctctgcg aggcggcctg
    33721 cccgtcacgc gttatccgcg tcgtcagcgc cgaagtgccg ggcgaaccga ccaagcgcta
    33781 cgccaaagaa tattatatgg acatgacccg ctgcctgttc tgcgggatgt gcgtcgacgc
    33841 ctgtcccgtg gacgcgcttg gcatgacccg tgagttcgag tgggccgtgt acgacaagcg
    33901 ccagctgcat ttgaacaaac agcagttgct cgcgattggc gatcgatcat tcccagttcg
    33961 cgagaagcgg ttggagctgc aacatccgaa cgtggccttc ttcaacgtcg cgttcggtca
    34021 tgtgccacag aaaccgaact gaacgtggcg agaccgcccg gcgggccatc gaggcagcca
    34081 accgggaaaa ggactgcctt cgatctggaa cgacatagca ccccggaatg atggaacagg
    34141 tcttcttttt ttattttgct ctcgttatcg cagtttcctc gatgctggtc gtcgcgctgc
    34201 gaaaccctat ctacagcgcg ctctcgctcc tgatcatgtt cttccacgtg gccgggctct
    34261 acgtcaccct ccacgccgag tttctcgccg ccgtccaggt gatcgtctac gcaggggcga
    34321 ttctcgtgct gtacctcttc gtcgtgatgc tgctcaacct ccatcaggac gaccgctacc
    34381 accagcaatg gcctgtcgcc gctgtcattg gactgacgct ggtcgtcgaa gccgtcatgc
    34441 tctcgctcgt gaagggacag gcgatcccgc ccgctccggc caacaccgag accgccgttg
    34501 agggcatcgg caatacggaa gcgttgggcg acgtactgta ctccacctac ctgtttccgt
    34561 ttgaagtcgc ctcgctgatt ctcctcgtgg ccatggtcgg ggccatcatt ctcgccaagc
    34621 gagacctgct ggagccgaag gaatcatgac cattcccttg tcctactatc ttattctgag
    34681 cgccatcgtg ttcctgaccg gcgtagtcgg cgtgctcatc agacgcaaca ttattatcat
    34741 tctcctgtca gtggaattga tgttgaatgc gacgaacatc aacttcgtcg cattttcgga
    34801 gtacttccac aacgtcgccg gacaggtctt cgtgttcttc gccctcaccg tcgccgcggc
    34861 cgaagtcgcc gtcgggttgg cgatcatcat cgccctgcac cgggccaagt ccaccatcaa
    34921 cgtggacgaa tttcagttgt tgaaatggta agcagggaac cgatggccta tagctcatgg
    34981 cgcatagcac aggcgaggag caaacgcttg tgccattcgc tatcagctct acgctcctag
    35041 aattttatga cgtacgaact cattcctctg cttccgctgg cttcgttcct gatcctcggg
    35101 ctcttcggcc attggatcaa ggacaaggct catctcgtgg cggtgcctgc cgtcgtgctg
    35161 tcatttctgt tctccatctt cgcctttatc gatgtcgcgg atgggcacca tacaacgttt
    35221 cgtctctata cctggctcac ctccggcaca ctcgacattc atatcggctt gacgatcgac
    35281 cggctcacgg cggtcatgct gctgctcgtc acaaccgtca gcgcgctcgt ccacatctac
    35341 acgatcggat acatgcaggg agaaccgggc tatgcccggt tcttctgcta tatcgccctg
    35401 tttaccttct ccatgttgat gctggtgctg gccgacaact tcctgcaact gttcgttttc
    35461 tgggaagccg tcgggctctg ctcctatcta ctgatcgggc attggtacga acgtccgtcg
    35521 gcctgcgcag ccgcgaccaa agcgttcatc gtcaatcggg tgggagactt tgggtttctc
    35581 cttggtctct tgctgatctg gaccaccttc aattcactgg actactcgga tgtcttcggg
    35641 aaggttcatg acgcagcagg acgcatgatc aatgtcctcg gaccgctggg aggcagttgg
    35701 gatgtgccgg tccttaccgt gatctgcctc ttgctgttta ccggcgcgat cggaaagtct
    35761 gcgcaagtcc ccctgcacgt ctggttgccg gacgccatgg aaggcccgac gccgatctcg
    35821 gccctcattc acgcggcaac gatggtcacc gccggtgtct ttctggttgc gcggctggcg
    35881 ccgatctaca acctctctcc ggcagccatg accgttgtgg cgatcgtcgg tgccctcacg
    35941 atggtgctcg gtgcgaccat tgcgttgacg caaaccgata tcaaacgggt cgtggcgtac
    36001 tcgacgatta gccagctcgg ctacatgata atggcctgcg ggttgggggc ctatgcctct
    36061 gggatctatc acttattgac gcacggggca ttcaaggctc tgctcttctt aggttgcggt
    36121 tcggtcatta tcgccttgca ccatgaacag gacatgcgaa agatgggcgg cctgaaagac
    36181 aagcttcccg tcacctattg gacctttgtg atcggctccc tagcgctggc aggctttccc
    36241 ctgacagccg gctttttcag taaagacgag ctgctggttt cggcctggtc atccggcacg
    36301 ctggggcaag tgttgacagt gttagggttg ttgacggctc tcatgacggc cttctatagc
    36361 ttccggctcg tcttcgtcac cttctggggt ccctcgcatg tcgatcatca ccatgcggcg
    36421 catgtgcatg agccgccgct agacatgacc gttccattga tcgtcctggc ggccctcagc
    36481 atcgtaaccg gctacctcgg tattccggag ttcctcgaac cgatgtttgc gagcgaatcg
    36541 ggcggcggtt cgaatcatgg gagtggcgca atcttcatca tgatagctgc cacggccatg
    36601 ggattgctcg gcatcgccgg ggcgtattac gtctatgtct tgaatccctc gctccccgat
    36661 cgctgggcca gacaatggca agcggcctat cagctttcgc tcaacaagtg gtacgtggat
    36721 gaagcctacg atcgaagcat cgtccgcccg acttttgacg cggcaacggg gctgtggaag
    36781 gggatcgatg tcgcgatcat tgacggcacg gtgaatggcg ttgcgcgggc cattgcatgg
    36841 ggaggctggc ttctgcggct cattcagagc ggacagaccc aacattatgc gttagggatg
    36901 gcgctgggcg ccgtcgtggt tttaaccctg tttatgatgt tctgaggact catgcaatcc
    36961 ggcggatttc cctggctgac tctgctgatc ctcctgccgc tggcaggggc cagcgtactc
    37021 tatctggcca aggattcgtc cgctcggctg attgcgctcg cagtgaccgt cgccgatttc
    37081 ctgttggcgc tacccctctg gtggctattc gatccttcgt cgagccaaat gcaatttgcc
    37141 gagcacgtga cctggattca ctcgccgccg attcattaca gcctggggct cgacggtatc
    37201 agcctgccgt tagtgctcat gactgccgcc ctgatgccgc tctgcgtctt gatctcgtgg
    37261 gacgcgatta cgacgaggat ccgcggtttc atggccatgc ttctgctgat ggaggcggcc
    37321 atgctgggtg tcttcgtcgc cgtggacttc gtgctgtttt acgtgttctg ggaagtaatg
    37381 ctgattccga tgtatttact gatcggggtc tggggcgggc cgaatcgatt atatgccgcc
    37441 atcaaattct ttctctatac cctcgcaggc agcgtcctgc tcctcgtcgc gattctcgcc
    37501 ctgtattttt acggagggca tacgttcgat atcctcgcgc tcagtcaggt cacctacccg
    37561 gcctcgctcc agatgtggtt gttcctggca ttctttgccg cctttgccgt caaggttccc
    37621 atgttcccgt tccacacctg gcttccggac gcccacgtcg aagctcccac tgccggcagc
    37681 gtgattttgg ccagcgtcct gttgaaaatg ggcacgtacg gattcttgcg cttcagtttg
    37741 ccgatgctgc cggatgcgag ccgggacatg acgccggtca tggccctgct gtcgatcgtg
    37801 gcgatcattt acggcgccta tatggcgctg gcccaaaccg atttgaagaa attgatcgcg
    37861 tactccagcg tcagccacat gggattcgtg acgctgggcc tgttcgtact caaccaacag
    37921 gggatcgaag gggcggtgct gcagatggtc aaccatggga ttacaaccgg cgccctcttt
    37981 ctctgtgtcg gcattattta tgagcgcacc cacagcagga tgatcgccga caacgtcggg
    38041 ctagccaagc cgatgccgca gtacgccaca ttgctcgtca tctttgcgtt gtcctcgctc
    38101 ggcctgcccg gcacgaacag ttttgtcggt gaatttctcg tgctcatcgg gacatttctc
    38161 tggagcaaga ccgcgacggc ctttgcctcg ctgggcatta ttctcgcggc agcttatctc
    38221 ctctggatgg ttcaacgcgt cgccttcggc attccctcgc cgcatcatct tcccaactta
    38281 aaagacttga gccggcgtga gtgggcaacc ttggcgccgc tcgtcgtgct ggtattttgg
    38341 atcggcctct acccgaatcc cctcgtcagc cggatgcatg cgagcgtgac gaaaacgtta
    38401 gacgctatgg ctcggacaaa agtggcgccg atatcgcacc cctcagcggc acaccctctt
    38461 cctgttgccg tgacctcacg tgatcttcag atcgaggaat cgagcaagcc atgacctcgt
    38521 atggcacgga cctactggtg attcttcccg agctgatcat cgtggcagca gcctgtcttg
    38581 tgctggcact cgatccggtg acgccctcat cgaagaaaca cctcctcgcc tggctcagcc
    38641 tcgcagccct ggccctttgt atcggtctga cggggggaca gatcagcgtg ttgaacatgc
    38701 gcgtgtttgc attcagcgat ctcgtcgtgg tcgacggata tgcacgcttc tggaaactgc
    38761 tgctctacgg tgtgacgggc ctgaccatcc tgatgtcgct cccctatctc aaggccgaac
    38821 ggctgcatct cggggaatac tacggattta tcctgttggc gctctccggc atgatggtaa
    38881 tggtctccgg cgccgatctc ctgacgatct atctgggcac agaattgatg tcgctctcac
    38941 tctatgtcat ggccggactc aaacggtcct cgccgcgttc gttggaggcc tcggccaagt
    39001 atttcgtgtt gggtgccttc tcatccggat tgctcctcta cggcatttcg ctcctctacg
    39061 gcttcgccgg cagcaccaag ctggtctcaa tcgccgcggc aatcggcact cgtggattcg
    39121 aggaccccat gatcttgatt gccacgattc tgttggtcgt gggattcggc ttcaaacttg
    39181 ccgttgttcc cttccacatg tggacgccgg atgtctatga aggcgcgccg acctcggtca
    39241 ccgcgttcat ggccgtcgca tcgaaggccg ccagcttcgg ggcattcctg cgtgtctttg
    39301 tcgagggact tggagggctc aaagcggatt gg
//
LOCUS       DATIFR010000186         1153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1270907, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000186 DATIFR010000000
VERSION     DATIFR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270907"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VLD60_10695"
     CDS             <1..921
                     /locus_tag="VLD60_10695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSB45473.1"
                     /translation="ITGTLSSRQIAVQLAPLTPSAPTPPVVSPPRATAPESPILNATS
                     PRPACKNPDALGVSRVVEIDTAGGPGFGFEQFKAYDFLSEREVVLTFDDGPWPGNTLA
                     VLKALADQCTKALFFPIGKHAMWHPEILKQVVAAGHTVGSHTWSHVDLSKLPVEQAKE
                     EFEKGASAVRAALGTSEASFFRFPGLRSPPEMLTYLGTRNVGIFSTDMDSFDFKIRKA
                     EQVVVSVMTKLKKRGKGIVLLHDFQHPTSLALPELLAQLKAGGYKIVQVRSKDPLTTL
                     PEYDAAIMKELGIGTVSTRPIANVVRTVSE"
BASE COUNT          245 a          345 c          309 g          254 t
ORIGIN      
        1 ataaccggca cgctctcctc gcgtcagatc gccgtgcagt tggcgccgct gacaccatca
       61 gctccgacgc cgccagtcgt ttctccaccg cgcgcgaccg ctccggaatc cccgatcctc
      121 aatgccacct cgccgaggcc agcctgcaag aatccggatg cgcttggtgt ctctcgcgtc
      181 gtcgaaatcg ataccgccgg cggccctggt ttcggctttg agcaattcaa ggcttacgat
      241 ttccttagcg aacgcgaggt ggtgctgact ttcgacgacg gcccgtggcc gggtaatact
      301 ttggcggtcc tcaaggcgct tgcagatcaa tgtacgaagg ccctgttctt tcccatcggc
      361 aagcatgcca tgtggcatcc cgaaatcctc aaacaggtag tcgcggcggg acacacagtc
      421 ggatcgcata cctggtcaca tgtcgatttg tcgaaattgc ctgtcgagca ggccaaggaa
      481 gaattcgaga aaggcgccag cgccgtccgg gcggcattgg gaacgtccga ggcatcgttt
      541 tttcgcttcc cggggctgcg gagcccgccg gagatgctca cctatcttgg gacgcgaaac
      601 gtcggcattt tttcgaccga tatggactcg ttcgacttca agattcgcaa agccgaacaa
      661 gtggttgtct ccgtgatgac caaattaaag aagcgtggca aaggcattgt cctactacac
      721 gacttccagc acccgacctc actggcactg ccagagcttt tggctcagct caaggcggga
      781 ggctacaaaa tcgtgcaggt tcggtcgaaa gaccccctga cgacgctacc ggaatatgac
      841 gcggcgatca tgaaggagct tggaatcggt acagtcagta cacgaccaat tgcaaacgtg
      901 gtgcgcactg tcagcgaata acagagcgcc ctcgctattc ggcgcactcc atcaatgggc
      961 catggctgcc actctcagat acaggtcttc gatgtatctc acggagcgca ccaagacggg
     1021 cttttagcgt ctcgggcatt ctctcgcgta cagatcgcga tttttcttcc actctgcgga
     1081 ccgccgtttc aaatgcaatc cattcactta taatccgtag tgcagatgct tgtgagccgc
     1141 ttttgtccaa att
//
LOCUS       DATIFR010000187        29610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1654038, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000187 DATIFR010000000
VERSION     DATIFR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 29610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654038"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..655)
                     /locus_tag="VLD60_10700"
     CDS             complement(200..655)
                     /locus_tag="VLD60_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HSB45474.1"
                     /translation="MVITCSDTRTPETDTSGQLIQKLLKEQGHTIVAYHLVKDEPAQI
                     KGHIAEAIANEAVQAIIINGGTGISRRDSTFEAVDAMLEKRLDGFGEVFRFLTYQDIG
                     SPAIMSRATAGIIKGRVLFSTPGSENAVRLAMEKLILPELGHLVKELTK"
     gene            complement(747..1073)
                     /locus_tag="VLD60_10705"
     CDS             complement(747..1073)
                     /locus_tag="VLD60_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSB45475.1"
                     /translation="MPKPKYHIVVCTNARPPGHPKPSCGSAGSAQLLMAFNMGLMQKG
                     IMPGEVLVTGSSCLGPCEQGPTVVVYPDNTWYSKVTEADVATIIDEHIKGGKPAEKLN
                     PDAVWK"
     gene            complement(1180..1623)
                     /locus_tag="VLD60_10710"
     CDS             complement(1180..1623)
                     /locus_tag="VLD60_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HSB45476.1"
                     /translation="MKFFLYGDNLNPTQLKRRAPEHQFLMLATLPDHTIQFSRWSSQW
                     RCGLATVAPSPGEKVWGAVFEVTDEDLKIMDKFEDDVPQGAFRHVQVTVVTEAGEKML
                     VTTYAATPIGKFKPKNHYLDWVMKGLKQWKFPEECIEQWQSFRPQ"
     gene            2057..2515
                     /locus_tag="VLD60_10715"
     CDS             2057..2515
                     /locus_tag="VLD60_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45477.1"
                     /translation="MVKIIHYVVVAILVLGAAYFIWSKPPTINPLIDRREADALAMVQ
                     THRAQGYPTILQAMTEHVRSMKDRGLNARLGEWRVKQVDGDLYEVRVQLRDQGKTGQW
                     FEREFIWHANLSLKKVNAASLPADGVTPKEDDSDQPAAPAQAGPKILGPT"
     gene            complement(2508..2828)
                     /locus_tag="VLD60_10720"
     CDS             complement(2508..2828)
                     /locus_tag="VLD60_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HSB45478.1"
                     /translation="MSDFVTVASTEELPPGTGKTVEVHGVWIALFNVGGSFYAIDNTC
                     PHAGGPLGEGYLTGEAVECPWHGWEFNVCTGERTGNPNFTVTCCPVRIEGNQVQVALP
                     PGFK"
     gene            2993..3538
                     /locus_tag="VLD60_10725"
     CDS             2993..3538
                     /locus_tag="VLD60_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSB45479.1"
                     /translation="MEMTLLLNSTYEPLRVVHWQKAIALLWQGKVEVLEVYDREVHAV
                     SISFKLPAVMRLLKLVRLKDSHRAVKFSRINIFTRDGYTCQYCRSKYRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNKKSGRTPEEAGMRLMKRPVKPRWSPIVTITIGIRNT
                     PESWRDYLYWNMELDTDSSGP"
     gene            complement(3595..4050)
                     /locus_tag="VLD60_10730"
     CDS             complement(3595..4050)
                     /locus_tag="VLD60_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45480.1"
                     /translation="MAGQNLRTLGFAGSIIGLSALAAWLGLAHSCDPQQGEAVIAGQV
                     LISPTLADQVRPSDVLFVIVKRPGGTPRPVAAKRIESPKFPVPFEITNADVMVQGSEL
                     RGMVDVIARLDRDGQAGPAQPGDIEGRYAKNPTLPGGRDFEIILDKVHE"
     gene            4188..4862
                     /locus_tag="VLD60_10735"
     CDS             4188..4862
                     /locus_tag="VLD60_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSB45481.1"
                     /translation="MAANPGFPLTLDVRGYPVLVLGGDEEAAEKTQRLLEAGAKVTVV
                     APSLHDQLKQLAASAKIVHRGRHFRETDLESAILVLNMVRGDKEFSSSLFAKAREKKF
                     LLWSVDQPESSTVNMPAVVASGHVRVAISTSGMAPALSAFIKEDLERILDHDFVAFVD
                     WLGLLREQSKANEPDAEKRRTMLREALDGFRLLGKVQYPKVWLDERAKQSVTSGVKGE
                     ASGAKP"
     gene            4902..5210
                     /locus_tag="VLD60_10740"
     CDS             4902..5210
                     /locus_tag="VLD60_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HSB45482.1"
                     /translation="MVLLEFSMSPLGKGESVGKYVARSLDIIDKSGVNYRLNPMGTVL
                     EGEWDDVMRVVTRCFQRMKRDCRRISCSIKIDYRKGHSGRLSSKVASVERRLKRKVRT
                     "
     gene            5455..7074
                     /locus_tag="VLD60_10745"
     CDS             5455..7074
                     /locus_tag="VLD60_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HSB45483.1"
                     /translation="MSAMKQLRWAMRSKLWIWIAAFKFSTNQDLDVVRLNKDQRPLKA
                     VPISQVYPSITINGVLVGERTPSDEQYPRFRYLLRRMFSRLFMLLNRLYPPMLSGLPS
                     IDPNPQTALGLAYTEQHRHAVAKAAKRHNKTVKEVLEPPALPLDLDVDGSPDLGGLAV
                     RGPFAGYLKKSDADGSFEWDLVHLGEYQPRPGLFRLGARVTFQLEADTKRLVPVTIKS
                     EIGLTRPADPAWPIARKIALCALSNHTSLIRHWNWVHLIGGEAVSIVTRNTLPERHPF
                     CRLLWPHVFGTQASNRLCTESQLVPGGDFEAVFSYGHEEMYRLFSDTVRGFDVQAWDP
                     EAYAIKRKVLHAGFAAPTEDNLRALYDVMHRHAARYLAIYYGNDAAVRGDRAICEWVD
                     GLNRALPNGLPFTSAEVTLSQLARVAASCMYLASVQHELVGTFLWNYQLWTYVLPVRV
                     YHDGKRDQLDVYQRLVNTNFMLNTPRAPLIADLTRLALREEAHPERYVMAVQAFETFK
                     RELEQLQEFMEREPWMPWKIYPKNLEININA"
     gene            7414..10086
                     /locus_tag="VLD60_10750"
     CDS             7414..10086
                     /locus_tag="VLD60_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45484.1"
                     /translation="MSVTMELFPPEQTILDRPFMQFVPFGVDEQGERIRDVSGMIVRD
                     NVEYLEELIRRSRGVESAGQSTAELCRLINDRVRDSAYHVTPGQLKNVWNSYSYEFTS
                     YLREFCRQLSGDPAFHQNVGREKHISPLIQTLGRPFSLAQIHRMYPYFAQKFAKGLEY
                     SVINVTDSSAVLRLTLTDRIYEQFGPYRKACMAQICESSKGRIEMVPPRLHRLPPSRV
                     SDRACIVKGDDYCEWEVRWEAQSQQHLSWAFWGIGAGVLTSVVTRLAYPSMSLGEVLL
                     AGLIPVLASGMLINRRLHRESRQREALIQEQINFVESRHEELREAYLEQEQTRVELRR
                     KVNHLTVLHRAGLLFSSTLDREALLQQVLETLTRDLRYDRAMISFFDPVRRVAMDARV
                     VGVSQEIQEFARAREIQVSDPLSPEGMVLLQGKPLLIGDVGAVWERLHPLNQHLVQVT
                     GTKSLMAVPLKAKDRILGSMMVGRMQEHSLTDEDLELLITFAHQVAIALDNVSAYQQI
                     EELNVGLEVKVRERTAELEQADRLRSQFLSHVSHELKTPLTSIKGFLQNMLDGLTGPV
                     NEKQQRYLARILENSERLIRMIEDLLDRTRIQTGRLELMPAELDVGQCVADVIEQLRP
                     LAQAKRQHLESQYPAVPLLVWADRDRLFQIVINLLQNAMKFTPEEGRIAVTVEQDGPR
                     HAGISVCDTGPGIPAEHLDKIFDPFFRIKQSRTGVKGLGLGLSIVRTLVELHGGSIQA
                     RNGPKGGAELYFTLPLRLPIDQSVRERDPGIPRILVVDDDNDIRQLLVDRLRAKGYEV
                     RAEADGKQALEAVRAERYSGMMLDIGLPSIDGMEVLRQIRQTDQQLPIIMMTASGAKE
                     SAVKAIGVGAQAYLLKPFDVDELQQIVESWFRTV"
     gene            10117..11595
                     /locus_tag="VLD60_10755"
     CDS             10117..11595
                     /locus_tag="VLD60_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSB45485.1"
                     /translation="MNEGLECDQLVTNDNQTCLVRLNRLRELVKIALMIGIVSLWSGT
                     FPDQTSAQVPRIQGQGTAASGMGNAFSAQADDPSALHYNPAGMTQLSGVQFMAGFLFA
                     GGTTNFTSSNPPTAGVNATGDRNGSLAWPPPGHTYLTANLKDLGVTALGDLTVGIGVT
                     VPFGSLTRWPDDGPFRNTVTFNTLPLLDIKPTVAYQLTNDLSVGLGADIYTFSGLFGE
                     GHVEQRFISPGGPATPLFGAAGSKVELNGKDTAAGFNASVLYTALRNGDGKPTANIGF
                     VYRSQATLHLSGALLSNGAKVQDATATLVLPQVMTGAIALWPVRTNEREWKIELDVDY
                     VGWKSVRNLDVHLANGTTIPQPQNWHSTYGVMVGTEYKWMNLESMPGWEVAARAGYTN
                     QQNQMPDLSFNPGIPSADVNIISTGLGLLCKENGSFFGLTQCGNLGIGVLKPKGIGID
                     LSYQAGLYEQRTVSGNTGLRAGVNGVYSSTYHTGGLSVRAMF"
     gene            complement(11636..12868)
                     /gene="alr"
                     /locus_tag="VLD60_10760"
     CDS             complement(11636..12868)
                     /gene="alr"
                     /locus_tag="VLD60_10760"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSB45486.1"
                     /translation="MAVQHGRSERRGDAYFLPYVEPLSDARTTLADFFIGLLTLHTTY
                     ATISLAALSHNLSQIQGRLSGGCGIIAVVKANAYGHGAVDISRTLIRQGISRLAVASV
                     QEGLALREAGITAEILVLVDMFDDHVEELVTRRLTPVVTDQRLLPALAKAAEAKQVSL
                     PIHIKVDTGMSRLGFPPEDLATLLDALLSWKSLRVEGFMTHLADGDGNDPAHTERQIE
                     SFRKMLDQVRKHGLIIPAIHAANSAAIVRFPQAHFSLVRPGIMLYGYHTLPNNIPAPT
                     LKPVLSLRTTVMQLRTIQPGDSVSYNRTFVASRKTRIAVLPIGYADGYSRRLSNKALV
                     LIKGRCAPVVGLVCMDMTMVDVTDVGAVQVGDEVTLIGEQGAEAIWADDLAEWSGTIP
                     YEILCAIGPRIPRVYHEG"
     gene            complement(12957..13520)
                     /gene="frr"
                     /locus_tag="VLD60_10765"
     CDS             complement(12957..13520)
                     /gene="frr"
                     /locus_tag="VLD60_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSB45487.1"
                     /translation="MSNPAAIRQAVTGKMEGALEHLKRDLAGLRTGRASVALLDNVRV
                     DYYGTMTPLRQVANLSTPEARLITIQPWEPQLIKEIEKALATSGLGITPSNDGKIIRV
                     PLPPLTEERRKELSKICKKHGEDTKVHIRGFRRDANEELKKLQKDAKLTEDDLRKSEV
                     EIQKLTDQYVQKIDEAIKKKEQEILEV"
     gene            complement(13538..14263)
                     /gene="pyrH"
                     /locus_tag="VLD60_10770"
     CDS             complement(13538..14263)
                     /gene="pyrH"
                     /locus_tag="VLD60_10770"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSB45488.1"
                     /translation="MSSAKYRRILLKVSGEMLAGEQGYGIQPSVLEGLAGEIAAVVAL
                     DVEVAVVIGGGNIFRGVAASASGMERASADYMGMLATVMNALALQNTLERTGIMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAAALRAMEIGAQVIMK
                     GTKVDGIYDADPVKNPSAKKYTEISFLSILNQDLKVMDATAISLCMDNRLPLVVFNLK
                     VPGNFKRVASGEPIGTIVTADQR"
     gene            complement(14260..14856)
                     /gene="tsf"
                     /locus_tag="VLD60_10775"
     CDS             complement(14260..14856)
                     /gene="tsf"
                     /locus_tag="VLD60_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSB45489.1"
                     /translation="MAGASQLVKELREKTGAGILDCQKALQETGDDIEKAVDYLRQKG
                     LAAAQKKSGRETNQGLIHAYIHMGGKIGVLIEVNCETDFVARNEEFKAFVNDLALQVA
                     AAKPLYVKREDIPKELVDKERAIYEGQAKEMGKPPAAWPKIVEGKLEKFYQESCLMEQ
                     AFIKDPAVMVKDLLSQKIAKIGENMNIRRFTRYQLGEA"
     gene            complement(14916..15689)
                     /gene="rpsB"
                     /locus_tag="VLD60_10780"
     CDS             complement(14916..15689)
                     /gene="rpsB"
                     /locus_tag="VLD60_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSB45490.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKKFLFGERNGIYIIDLQ
                     QTVARMEQAYAFVRDTVASGQSVLFVGTKRQAAEILQEEAVRANMFYVNQRWLGGMLT
                     NFQTIRRSIDKMKKLEGMLADPAQHGLTKKEISQKQKEIVKLQKFLTGIKNLRGLPGA
                     VFILDTRIERIAVQEATRLDIPVIAIIDSNCDPDHITYPIPGNDDAIRSIKLITSRIA
                     DACIEGAHLRAQRDEAEFQASPAAVDKGVAPSLAAAPAS"
     gene            complement(15916..17124)
                     /gene="argJ"
                     /locus_tag="VLD60_10785"
     CDS             complement(15916..17124)
                     /gene="argJ"
                     /locus_tag="VLD60_10785"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSB45491.1"
                     /translation="MTTLNSSGVTAPQGFLASGLHCGIKKPGLLDLALVVSERSGPIA
                     GVFTNNQVVAAPVILDRRHLRRGMGRAILVNSGNANACTGASGLKAAEKTAALVARAL
                     CIPVHEVFVGSTGVIGRALPLDRIVKAFPALLKNLSSRAGMQAARAIMTTDLKPKTVV
                     RRQKINGRVVTIGGMAKGSGMIHPNMATMLGYLTTDAAITRKALQQALTAAVGQSFNC
                     ISVDGDTSTNDTVLCLANGMANNRIIKEGTPAFRQFVHLLTEACQPLALAICRDGEGV
                     TKVVKIEVTGARTVQQARQVAQSIGTSNLVKTALFGEDANWGRVMAAIGRAGVPLNPS
                     TINVRFGGVPMVRHGVGLGLVAEQRIAKVFRHKEFTIAVDLGQGRHQAYIWTTDLSYD
                     YIRINASYRS"
     gene            complement(17158..18234)
                     /gene="argC"
                     /locus_tag="VLD60_10790"
     CDS             complement(17158..18234)
                     /gene="argC"
                     /locus_tag="VLD60_10790"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSB45492.1"
                     /translation="MMRKIRVAIAGASGYAGAELVRLAALHPYFELVAVTSEKSAGSP
                     VASIFPSLAGLVSLSFQALAPEAIAEQADAVFLALPHTKSLAPVSACMRTGKFVVDLS
                     ADYRLKDPAAYERWYQTPHTHAHLLKDAVYGLPELHRAEIAKSKLVASPGCYPTAAIL
                     QLAPLFAHNMVQAQTVVIDAKSGISGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEI
                     EQELGGLLQRHTGSSTIDPVSSTVTFTPHLVPMNRGILSTAYCRLKQPMALPDIRALY
                     RTFYKNERFVRLSEEVVPNPRYIKGSNYCDIGVYVDQRAGWIVTVAAVDNLVKGAAGQ
                     AIQAMNLMLGLPEETGLTAPAAYP"
     gene            complement(18328..18720)
                     /gene="rpsI"
                     /locus_tag="VLD60_10795"
     CDS             complement(18328..18720)
                     /gene="rpsI"
                     /locus_tag="VLD60_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSB45493.1"
                     /translation="MAVATQYATGRRKCAIARAWVTGTAGDITINDQPLEKAFPRLTL
                     RQIIQLPLELAGVAGKFSIKATVHGGGPTGQAGALRHAIARALVSLNEPLRAPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(18755..19183)
                     /gene="rplM"
                     /locus_tag="VLD60_10800"
     CDS             complement(18755..19183)
                     /gene="rplM"
                     /locus_tag="VLD60_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSB45494.1"
                     /translation="MSTYLQKPADVQEKWYLVNAEGKTLGRLAARVAGVLRGKHRPTF
                     TPNVDMGDHVVIVNAEKIQLTGDKMETKLYRSHSGYPGGLKTATAKHVFRKDPTQLLV
                     AAIEGMLPKTPLGNHMAKKLRVYVGSAHPHQAQNPETITL"
     gene            complement(19216..19719)
                     /locus_tag="VLD60_10805"
     CDS             complement(19216..19719)
                     /locus_tag="VLD60_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSB45495.1"
                     /translation="MDDIGHSGSTKDQALIEYKVDRVLDETNTDTKIVVLARHDGQQD
                     SFPIWVGAAEGHAIKLALDTTITPRPMSHDLIKSFIDHLGITVRRIVIADVKNSTYYA
                     MVHLENHGVERAVDARPSDALSLALRTHAPIYITQDVLARRSTANLDSWLAKLDSKNI
                     KQEVQEP"
     gene            complement(19725..20180)
                     /locus_tag="VLD60_10810"
     CDS             complement(19725..20180)
                     /locus_tag="VLD60_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSB45496.1"
                     /translation="MITQMHLKGLMFDPYNNAYIVVLRDEEGADMLPIWVGKAEAGAI
                     SMALENVAPPRPMTHDFMKSILDAYDAKVLSVVITDLNENTYFAKIHLMYEDSEYTVD
                     ARPSDAVALALRSNAPIFAKESVLNKQSSEELEQWLENLKPEDFGKLDS"
     gene            complement(20351..20632)
                     /locus_tag="VLD60_10815"
     CDS             complement(20351..20632)
                     /locus_tag="VLD60_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HSB45497.1"
                     /translation="MAEVQCVTCGQAGEPITDTLFMGKLEAEIKSKVCKPCWKKWESM
                     RVMVINEYQVNLGDESGRELVKKQMKAFLKIGGEPADTSKLDQNYRPQA"
     gene            20768..20854
                     /locus_tag="VLD60_10820"
     tRNA            20768..20854
                     /locus_tag="VLD60_10820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20802..20804,aa:Leu,seq:caa)
     gene            21055..21795
                     /locus_tag="VLD60_10825"
     CDS             21055..21795
                     /locus_tag="VLD60_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45498.1"
                     /translation="MRNVLALGAAVLLVGSIGVAGAQERLQSNQSGMGVGTGVGDVSG
                     SSTRVTTFDRSNFLDRLSQAETIYGRVLAIDIPAKKMHLEGGGSSHDEGRAGGGAMNY
                     RTVYFDDHTNMDQIRVIETGDDVTIQAFEETTASQPYGTGKKLVREITVLRGNEKLAG
                     FGGLGQRPNPMTERGIETNSGGMTGGVAGGVLPGKIDSGITSSVGEYTGAAPCWQCEP
                     QPGWGNNVGNKSDYGADYTKPNLVKGLN"
     gene            21957..22214
                     /locus_tag="VLD60_10830"
     CDS             21957..22214
                     /locus_tag="VLD60_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSB45499.1"
                     /translation="MSGQGQSNRPDAELDLRGVICPYNFVKTKLKLEAMEEGQILSVI
                     LDAGDPIRNVPQSVSNEGHTILSQEPIDHSYRVVIRRRADE"
     gene            complement(22189..22986)
                     /locus_tag="VLD60_10835"
     CDS             complement(22189..22986)
                     /locus_tag="VLD60_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase I"
                     /protein_id="HSB45500.1"
                     /translation="MAPQRSQPIYPEWQDPASRRTGTLYIVGTPIGSFDDLSIRALNT
                     LKNVSIVAAENPLATQALLDHHGISATITSYGPRNHDEKISVLLHALHRGQHVALVSD
                     SGMPVIYDPGRLLIAAAQQAGYPVTVIPGPSALTAAVAISGYSGDRVHFEGKFPRTKR
                     LANRFLAHFKGNSVTLVFFAPSAHCAHILQSLHRILPDRQVTLATDMTRTTERLCQGT
                     AASLLAQGRPLAENAEVTVVVEGEPPKGVRRTRLRATRHATRRRGAE"
     gene            23048..23851
                     /gene="ccsA"
                     /locus_tag="VLD60_10840"
     CDS             23048..23851
                     /gene="ccsA"
                     /locus_tag="VLD60_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSB45501.1"
                     /translation="MAAVFFMVTMALYFAATVSFLALLLRRADALSTVSLTITSIGFV
                     THTIALVARMVGATMAAQPSIHEALSFFSWMLILVFLAVEFRHRLHVLGSFIVPLALV
                     SLVSAAALPETVPTLQPMFRTLWIHVTLSMLGTVGFAVAFVAGVMYLIQDGLLKSKRF
                     NVLYSKLPALDYLDELNQQSMVLGFPLLTLGIITGAISAEFARGAYLNWNPEQTWALV
                     TWLFYFIVLTGRLTVGWRAKRAAYLTIIGFACVILTLVAVAVKSFGSVS"
     gene            23853..25196
                     /gene="hemA"
                     /locus_tag="VLD60_10845"
     CDS             23853..25196
                     /gene="hemA"
                     /locus_tag="VLD60_10845"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSB45502.1"
                     /translation="MHIVVVGLSHKTAPVEIREKLAIPESRIGEALTRLCSYSGVKEG
                     MLLSTCNRVEVYAVVEDLESGYSRIQEFLADAHLSLSSEQLTPHLYWQSGDRAILHLF
                     RVASSLDSMVVGESQILGQLKDAFEMALTHKTTGLILNKVVKKAISVAKRVRTDTKIA
                     EMAVSVSYAAVELAKKIFADLSDKTVLLVGAGEMAKLAARHFIANGVSHVRVTTRTPQ
                     NAVELADRFNGTAVPFEHFREDMAAADIVLVSTGASHYLVGSDDVQRAVKQRMNRPIF
                     LIDISVPRNIDPAVRHVDNAFLFDIDDLKSRVEQNRGERLQEAEKAEHMVVEEVSVVR
                     QWLQSLEVTPTIVALKTRVEDIRRAEVEKVLGRLGNLSPQERALVESMASSIVNKLIH
                     NTMVTLKSEVTSADGAAFVEAARRFFNLSDSGPALLTEEVETEGTTRRSGGNEPN"
     gene            25223..25546
                     /locus_tag="VLD60_10850"
     CDS             25223..25546
                     /locus_tag="VLD60_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45503.1"
                     /translation="MGLAAKGPKLFTCPNCRKAYLGYDPLPDCPACGFDYRVRAGFRW
                     DVLVYLLSILGLMSYFLVSTSYRSLITEPISNERGVSAVSGEEKLPGSRTLPMSYPAE
                     PRASH"
     gene            25556..26503
                     /gene="hemC"
                     /locus_tag="VLD60_10855"
     CDS             25556..26503
                     /gene="hemC"
                     /locus_tag="VLD60_10855"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSB45504.1"
                     /translation="MTHSIQARSQLVLGTRGSKLAVHQSEWVQARLKELAPHVTVTLR
                     RIHTAGDKILDVPLAKIGGKGLFVKEIEEALLSGEIDLAVHSMKDVPTELPDGLELLC
                     VPLREDPRDALISRDGRLFKDLPRGAKVGTSSLRRQSQLLQARPDLSISMLRGNLDTR
                     MRKLREGQFDAIVLAAAGLRRLGWEHEITEYLAPEISLPAIGQGALGIEGRRDDGFVR
                     SLLAGLEHAPTRTMVLAERALLHRLQGGCQVPIAAHATVHGSDVTLEGLVASVDGKEV
                     IRETVRGTVADPESMGIQLAERLLARGGDRILQTIYGTS"
     gene            26503..28065
                     /gene="cobA"
                     /locus_tag="VLD60_10860"
     CDS             26503..28065
                     /gene="cobA"
                     /locus_tag="VLD60_10860"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSB45505.1"
                     /translation="MGTTRRGKVYLVGAGPGDPGLLTLKGKECLEQADVVLYDYLANP
                     VLLQHTREQAERIYVGRRGRGHYQDQADINRLMIERAKAGQVVVRLKGGDPFVFGRGG
                     EEAEAVAAAGVDFEVVPGVTAAVAVPAYAGIPVTHRTLASTVTIVTGHEDPSKQTTVM
                     DWPKLAATSGTLVFMMGMKTLPTIVARLLEEGRSPQTPVAAIRWGTRADQQTVVGTLK
                     DIVEKAEQARFEPPTVIVVGDVVGLREQLNWFEKRPLFGKRIVLTRAQEQAQEFSRLL
                     AAYGAETIEVPTIQIVPPVSWQAIDHAIARLASYQWLMFTSVNGVKPFMERLRAAHKD
                     SRALAHLQVCAIGPRTAAELEQYGLRPDMVPLEYQAEGILAVFEGQDLRGKHVLIPRA
                     EVAREFLPEQLRRMGAEVDVIPVYRTTVPAADLSRVREQIEAGKIDLVTFTSSSTVRN
                     FVELVGGSDEARRLGTKTAIGCIGPITAQTAAEYGLSVAIVPPENTVLALAHAIVRHF
                     SEGARIEVSALR"
     gene            28114..28506
                     /locus_tag="VLD60_10865"
     CDS             28114..28506
                     /locus_tag="VLD60_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB45506.1"
                     /translation="MVPVKSFMVPKEKFITVERDTDAQTAARIMRDRGIGSLFVTNGK
                     EIVGIITDTDMVRRVVAPGVDTHKTTVEQIMSAPIMTIEDNKTLLDANDLMAQTHLRH
                     LGVTKDGKLVGMISVRDLVVFLTNLPRK"
     gene            28512..29483
                     /gene="hemB"
                     /locus_tag="VLD60_10870"
     CDS             28512..29483
                     /gene="hemB"
                     /locus_tag="VLD60_10870"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSB45507.1"
                     /translation="MAFPIQRLRRLRQHEALRRMVRETSLSPSDLIYPIFVGEGRDRR
                     EEIASMPGQFRLSIDLLVKEAGEVAGLGIPAIILFGIPDRKDERGSSGFDPNGIVQRA
                     IKAVKDQVPGLMVITDVCIDEYTSHGHCGIVKNGRILNDETLDCLRAMARTHAEAGAD
                     MVAPSDMMDGRVAAIRDELDRAGYPDMPIMAYAAKFASCFYAPFREAAGSSPQFGDRQ
                     SYQMDPANKREAMREIALDIEEGADIVMVKPAMPYLDVIHRVKAEFDLPVAAYQVSGE
                     YSALVAAHRNGWLDRERTILESLTCIKRAGADMIITYFAKDAAKLVG"
BASE COUNT         5947 a         8029 c         8994 g         6640 t
ORIGIN      
        1 gggatctgct gatgttctac aagcggtatg cgcaatcgaa gcttcccccg gtgatggagt
       61 attcggaacc gaagattatg caggactgac ggcggcacag agtaaagggt gaggagtgag
      121 gagtaagcgg caaaaaagag aagtgcgcgg tccgaacagg tcaggagtac cccctcactc
      181 ctaactcctc actcttcacc tatttcgtga gttctttgac caggtggccg agttccggaa
      241 ggatgagttt ctccatcgcc agccggacgg cgttttcaga tcctggcgtg gaaaagagca
      301 cgcggccctt gatgatgccg gcagtcgccc ggctcatgat ggccggggaa ccgatgtcct
      361 gatacgtcag aaagcgaaag acctcgccga atccatccag ccgtttttcc agcatcgcat
      421 cgactgcctc gaaggtcgaa tcccttcgtg agattccggt cccgccatta atgatgatgg
      481 cttgcaccgc ctcgttggcg attgcctcgg cgatatgacc tttgatctgc gccggttcgt
      541 ctttaacaag atggtaggcg acgatggtat ggccctgctc cttgagcagt ttctggatca
      601 actgtccgct ggtatcagtt tcgggcgtgc gggtgtcgct gcaggtgatg accatgcagc
      661 ccaccttcct gggtgcgtgg gctttatgtt cttgagcggc gtaacttccc atttatttat
      721 agtacgctga acgtggcggc tttttcttac ttccagactg catcggggtt gagtttttcc
      781 gccggcttgc cgcccttgat gtgctcgtcg atgatggtcg ccacgtcggc ttccgtcacc
      841 tttgaatacc aggtgttgtc gggatagacg acgacggtcg ggccttgctc gcaggggccg
      901 aggcaggagg aaccggtgac gaggacttca ccaggcatga tgcctttctg catcagaccc
      961 atattaaagg ccatcagcaa ttgagctgag cccgcgcttc cgcaagacgg cttggggtgg
     1021 ccggggggac gggcattggt gcagacgacg atgtggtatt tcggctttgg catgaaatct
     1081 ccttgattcg aagagccgtc agcggtcagc tgtcagctct cagctgttag ggccgggaag
     1141 ttgttcggct gatcgctgaa ggctgattgc ttccctgctt cattgtggac gaaacgactg
     1201 ccactgttcg atgcattctt ccggaaactt ccactgcttg aggcccttca tgacccaatc
     1261 cagatagtga ttcttgggtt tgaatttgcc gatcggcgtg gctgcgtagg tggtgaccag
     1321 cattttttcg ccggcttccg tgacgacggt cacttggacg tgccggaagg ccccttgcgg
     1381 cacgtcgtct tcgaacttgt ccataatctt gaggtcttcg tcggtgactt cgaagacggc
     1441 tccccagact ttttcgcccg gcgagggggc gacggtggcc agtccacacc gccattgcga
     1501 cgaccagcgg gagaactgga tcgtatggtc gggcagggtt gccagcatca ggaactggtg
     1561 ttccggggcc cgccgcttca attgagtcgg attgagatta tcgccgtata aaaagaattt
     1621 catctggtgt cctgctcaca attagactgc tcaagggcag cgctacttgt agcatacggg
     1681 ctttcgctcg cacaagccgc tagcaggctg ttgaaaaggt ccgtcagcgg cgttctcgcg
     1741 gcgctcaagt cctcaacgta ccgatcctac tggaggccaa gtacctatcc gctcgcatgt
     1801 gatcgaagcg agcggttcaa gcgaagcttg gtatgtacct cctcggccct tcactcgctg
     1861 cggccttcag ggggtagagg cgcgtcttgg cgcgcccagg gctgggcggg tgagaagggt
     1921 cggccctttt gaacagcctg cagataaaac gctcatttcg agtgcttcag atcagggcag
     1981 attgacttcg agcgagcgct cttcctatga tgcccatcat gcatgcaagt gatgcccgtg
     2041 caggcgattc cgctccatgg taaagatcat tcattatgtc gtcgtggcga ttcttgtgct
     2101 cggcgcggcc tattttatat ggagcaagcc tccgaccatc aacccgttga tcgatcggcg
     2161 ggaggcggat gccttggcga tggtgcagac gcatcgggcg cagggctatc cgacgatcct
     2221 gcaagccatg accgagcatg tgcggtccat gaaggatcgc ggtctgaacg cgcggctcgg
     2281 tgaatggcgg gtgaaacaag tcgatggcga tctctatgag gtccgcgtgc agttgcggga
     2341 tcaagggaag accgggcaat ggtttgagcg ggaatttatc tggcacgcca atctgtcgtt
     2401 gaagaaggtg aatgccgcgt cgcttccggc tgatggcgtc accccgaagg aggacgactc
     2461 ggatcaaccg gctgcgcctg cgcaagccgg tcccaagatt ctcggtccta cttgaagccc
     2521 ggcggcaggg cgacctggac ctgattgcct tcaatgcgaa cggggcagca ggtcactgtg
     2581 aagttggggt tgcctgttcg ttccccggtg cagacgttga attcccatcc gtgccatgga
     2641 cactcgacgg cttcgccggt cagatagcct tctccgagcg ggcctcccgc atgggggcag
     2701 gtgttgtcga tggcatagaa ggagccgccg acgttaaaca gggcgatcca aactccatgg
     2761 acctccaccg tttttcctgt tcccggggga agctcctcgg tggaggcgac agttacaaaa
     2821 tcagacatgg atcctcatgc ggcggccgga ccgaatcctt gggagggccg gcataacagc
     2881 ccaccgcttg attttacagg ggtcttgtgg catacaaata ccagagcgta cccagaccga
     2941 tagaaggccc tctcagacaa ggagcctcgg ttgggaatga ggattccact ctatggaaat
     3001 gaccctcctg ctcaattcga cctatgaacc cctgcgtgtt gtccattggc aaaaagcgat
     3061 cgcgctccta tggcaaggaa aggtcgaagt cctcgaggtt tatgaccgcg aggtgcatgc
     3121 ggtctccatc tcgtttaagc ttccggccgt catgcggctc ctcaagcttg tccgcttgaa
     3181 agacagccat cgggcggtca agttctcccg tatcaatatt ttcaccagag acgggtatac
     3241 ctgccaatac tgtcgcagca agtacagaac ggaagagctc acgttcgacc atgtcgtgcc
     3301 gatcgccaaa ggcgggcgca agacatggga gaatatcgtc acggcctgct ggcgctgcaa
     3361 caacaagaag agcggacgca cccccgagga agccggcatg cgtttgatga agcggccggt
     3421 gaaaccgcgc tggagtccga tcgtcaccat taccattggt atcagaaaca cgccggagag
     3481 ttggcgggat tacctgtact ggaatatgga gctggataca gattcctccg gaccgtaacg
     3541 tgctgagcga tcgactctcc tcacccaagc ctcaacacgt tccttccgac ttcactactc
     3601 gtgcacctta tctaagataa tttcgaagtc tcgcccgccc ggcagcgtcg ggttcttcgc
     3661 ataccgtcct tcaatgtcgc ccggctgagc tgggccggcc tggccgtctc gatccaatcg
     3721 cgcaatcacg tccaccatcc cacgcagttc cgaaccttgc accatgacgt cggcattggt
     3781 gatctcgaac gggacgggga atttcgggct ctcgatccgc ttcgcagcga ccggcctcgg
     3841 cgtccctccg gggcgcttca caatcacaaa caacacatcg cttggacgca cctggtcggc
     3901 cagtgtcgga ctgatcagga cctggcctgc gatgacggct tcaccctgtt gagggtcaca
     3961 actgtgggcg aggccgagcc aggctgcgag cgccgacagg ccgataatgc tcccggcaaa
     4021 gccgagggtg cggagattct gtccggccat caggaaactc caatccgtga agggtggttc
     4081 agaaacggcg cattataccg atgtggaaaa gaaaagggga gtgcatggtt tacgctggaa
     4141 aaggctctgt tgtatgatgt cccttctttg gaactggagg attcaccatg gcagcaaatc
     4201 ctggcttccc cctcacactc gatgtcagag ggtatcccgt cctcgtgctc ggtggagacg
     4261 aagaggccgc agaaaaaacc caacgtttgt tggaggccgg cgccaaagtc acagtggttg
     4321 caccctcgct gcatgaccag cttaagcaat tagcggcctc ggcgaagatc gttcaccggg
     4381 ggcgtcattt tagagaaacg gatctggaaa gcgcgattct ggttctcaat atggtgcgcg
     4441 gagacaagga attctccagt tccctgttcg ccaaggcgcg cgagaagaag tttctcctct
     4501 ggtccgtcga tcagccggaa tcgtcgactg tgaacatgcc ggcggtcgtt gcgtcggggc
     4561 atgtgcgcgt ggcgatcagc acgagcggca tggcaccggc gctctcggca ttcatcaagg
     4621 aagacctcga acggattctg gaccatgact ttgtggcatt tgttgactgg ctcggcctgt
     4681 tgcgggaaca gtccaaagcc aacgagccgg atgccgaaaa acgccgcacg atgttgcgcg
     4741 aggcattgga cgggttccga ctcttgggga aagtgcaata tccgaaagtc tggcttgatg
     4801 aacgtgcgaa gcagagcgtg acgtcaggcg tcaagggtga ggcgtcaggg gcaaaacctt
     4861 agagttgatc gctgacagct gatagcaagg agtaactgtt catggtgcta ctcgaattca
     4921 gtatgtcacc gctcggaaaa ggtgaaagcg tcgggaagta cgtggctcgc tcactggaca
     4981 tcatcgataa aagcggagtg aattatcgat tgaatccgat gggcaccgtt ctcgaagggg
     5041 aatgggacga cgtgatgcgg gttgtgactc gctgcttcca acgaatgaag cgagactgcc
     5101 gccgcatttc ctgttcgatc aagattgatt accggaaggg ccattccggc cgtttgtcga
     5161 gcaaggtggc gagtgtcgaa cggcgcctca aacgaaaagt aaggacctag accagcggga
     5221 ccggttctgt tgccccctca ctgtatcctt tctcccgcgc gtgtatctca tgtgatacct
     5281 cctcgctcga ttcaacggcg tttccgttgg aacgaacggt gtatgagccc ataattcccc
     5341 tcgtttgccg cattgcttgt cgcacgcctc atgggagtgt aggggtatgg cgcttgcctt
     5401 ctctatgtgg gtgacgatcg atcgatccgg acattcatcg aggagcgaac ggggatgagt
     5461 gcgatgaagc agctccggtg ggccatgcgg agcaagctgt ggatatggat cgccgcgttc
     5521 aagttctcga ccaatcagga tctggatgtg gtccgtctca acaaggacca gcgcccgctc
     5581 aaggctgttc ctatttcgca ggtctatcca tcaattacga tcaacggcgt gctggtaggg
     5641 gaacggacac cctccgatga gcagtaccct cgatttcgat acctcctgcg gcggatgttt
     5701 tcaagactgt tcatgctgct caatcgtctg tatcctccga tgctatcggg ccttccgtcc
     5761 atcgatccca atccgcaaac cgccctcggc cttgcgtata cggaacaaca tcgccatgcc
     5821 gttgccaaag ctgcgaaacg gcacaacaag acggtcaaag aggtgctgga acctccggca
     5881 ctgccgcttg atctcgatgt ggatggtagt cccgatctcg gggggttggc ggtcagggga
     5941 ccatttgccg ggtacctcaa gaaatccgac gccgatgggt ccttcgaatg ggatttggta
     6001 catctcggtg aatatcagcc aagaccaggc ctcttccgct taggggcgcg cgtcacgttt
     6061 cagctcgaag ccgataccaa acggcttgtt ccagtcacga tcaaatcgga gatcggactg
     6121 actcgtcctg ccgatccggc ctggccaatc gccagaaaga tcgcgctctg tgcgttgtcc
     6181 aaccacacca gccttatccg ccactggaac tgggtccatc tcatcggcgg cgaagccgtc
     6241 tccatcgtga cgcgcaacac ccttcctgag cggcatccct tctgccgcct gctctggcca
     6301 cacgtgttcg ggacgcaggc gagcaatagg ctgtgcaccg aatcgcagct cgtgccgggc
     6361 ggggattttg aagcggtttt cagctacggc catgaggaaa tgtatcggct ctttagcgac
     6421 acggtgcgcg ggttcgatgt gcaagcctgg gatcctgaag cctacgccat caagcggaag
     6481 gtgctccatg cggggtttgc cgcgccgacc gaagacaatc tgcgggctct ctatgacgtc
     6541 atgcatcgcc acgccgcccg gtacctcgcg atctattacg ggaatgatgc cgcggttcgt
     6601 ggtgatcgcg cgatctgcga gtgggttgat gggttgaacc gcgccttgcc gaatggactg
     6661 ccgttcactt ccgcagaggt gacgctttcc cagctcgcgc gagtagccgc ctcctgcatg
     6721 tatctggctt cggtccagca tgagctggtc gggacattcc tctggaacta ccagttgtgg
     6781 acctacgtgt tgcctgtccg tgtgtatcac gatggaaagc gggatcagct ggatgtctac
     6841 caacgtctgg tcaatacgaa ttttatgctg aatactccgc gcgcgccgct gatcgccgat
     6901 ttgacgaggc tggctctgcg tgaggaggcg catccggagc ggtatgtcat ggccgtccag
     6961 gcgtttgaga cgttcaagcg cgagctggag cagctccagg aattcatgga gcgcgagcct
     7021 tggatgccgt ggaagattta tcccaagaat ctcgaaatca acatcaacgc gtaacagaat
     7081 gctgaaaaag tccgccagcg gcgttctcgc gttgctcaga gactccatgt acggcacaaa
     7141 gtacgcgtcg tctcttcgct cgctgcggcc ttgccggacg gcctttttga gcatcctgca
     7201 agaggctttt gtgccgtcgc tgcgcttgaa tggccaagtc acacacggca tgggttggca
     7261 gctccagatc atactggttg ctcatcgtat caatgggcgt tcgaagcccg caggacgcgg
     7321 ttgcccttta ctctccaatc agcggcggtg cacaattgcg ggccttcttt cggtgacgcc
     7381 cggtgcgagt cattcaagca ggatgagtcg acgatgtccg tgacgatgga attgttccct
     7441 ccagagcaaa ccattcttga tcggcccttc atgcagttcg tgccattcgg cgttgacgaa
     7501 cagggcgaac gcattcgtga cgtcagcggc atgatcgtac gtgacaacgt ggaatatctg
     7561 gaagagctga tcagacggtc acggggggtc gaatctgccg ggcaatcaac ggctgaactg
     7621 tgccggctca tcaatgaccg cgtccgggat tccgcctacc atgtgactcc cggacagttg
     7681 aagaatgtct ggaacagcta ttcctatgaa ttcacatcct atctacgaga gttttgtcga
     7741 caattgtccg gtgatcccgc ctttcatcag aacgtcggcc gggaaaaaca catttctcca
     7801 ttgattcaga ccctggggcg cccgttcagt cttgcgcaga ttcaccgcat gtacccgtat
     7861 tttgcacaga agttcgccaa gggcctcgag tattccgtga tcaacgtgac ggattcttct
     7921 gccgtgctca ggctgaccct gaccgaccgg atctacgagc agttcgggcc ctatcgaaaa
     7981 gcctgtatgg cgcagatttg cgaatcgtcg aagggacgga tcgagatggt gccgccccgg
     8041 ctccatcggc ttccaccatc tcgggtgagc gatcgtgcat gcattgtgaa gggagacgat
     8101 tactgcgaat gggaagtccg ctgggaggcc cagtcgcagc agcatctgtc ctgggctttc
     8161 tgggggatcg gagccggtgt gctgacatcg gtcgtcacgc gcctggccta tccgtcgatg
     8221 agccttggag aagtactcct ggcaggcctg attcccgtcc tggcgtcagg gatgctcatc
     8281 aatcggcgtc ttcacaggga gagccgccag cgagaggcgt taatccagga gcagatcaat
     8341 ttcgttgagt cccgccacga agagctccgg gaagcgtatc tggagcagga acagacacgt
     8401 gttgaactgc gccggaaggt caatcatctg accgtgctcc atcgcgccgg gttgttgttc
     8461 agctccacgc tggatcgcga ggccttattg cagcaggtgt tggagacgct cacgcgcgat
     8521 ctccgctacg atcgggcgat gatctccttt ttcgatcctg ttcgacgggt cgcgatggat
     8581 gcccgcgttg tcggcgtgtc tcaggagatc caggaatttg cgagggccag agaaattcag
     8641 gtttcggatc ccctcagtcc ggagggaatg gtgctgttgc agggcaagcc gctgctcatc
     8701 ggcgacgtag gcgcggtgtg ggaacggctg cacccactga atcagcatct ggtgcaggtg
     8761 accggaacca aatcgctgat ggctgtgccg ttgaaagcaa aagatcggat tctcggaagc
     8821 atgatggtgg ggcggatgca ggagcacagc ctgaccgacg aggatctcga gttattgatc
     8881 accttcgccc atcaggtcgc catcgcgctg gacaatgtgt cggcctatca gcagatcgag
     8941 gagttgaatg tcggcctcga agtgaaagtg cgtgagcgga ccgccgaact ggagcaggcg
     9001 gatcgattac gatcgcaatt tctctcacac gtgtcgcatg agctgaaaac accgctcacc
     9061 tcgatcaagg ggtttctgca gaacatgctc gatgggctca cggggccggt caatgaaaaa
     9121 cagcagcgct atctggcaag gatcttggag aattcggaac gattgatccg gatgatcgaa
     9181 gacctgctgg accggacgag gattcaaacc ggcagactcg aactgatgcc tgccgagctg
     9241 gatgtggggc agtgtgtagc cgacgtgatc gaacagcttc gccccctcgc ccaggcgaaa
     9301 cggcaacatc tggaatccca gtacccggcc gttccgctcc ttgtgtgggc ggatcgagat
     9361 cgcctgttcc aaatcgtcat caacctgtta cagaatgcga tgaagttcac gccggaagag
     9421 gggcgcatcg cggtgacggt cgaacaggat ggtccccggc atgcaggaat ctccgtgtgt
     9481 gataccggac cgggaattcc ggcggagcat ctcgataaaa tcttcgatcc tttcttccga
     9541 atcaagcaat ccaggaccgg ggtcaaagga ttggggctcg gattgtcgat cgtccggact
     9601 ctcgtcgagc tgcacggcgg ctcaattcaa gcccgcaacg ggccgaaggg aggagccgag
     9661 ctgtatttta cccttcctct gcggctgccg atcgatcagt cggtgcgcga aagagatcca
     9721 ggcatccctc gcatcctggt cgtggacgac gataacgaca ttcggcaatt gctggtggat
     9781 cgtctgcgtg ccaagggcta cgaggttcga gccgaagccg atgggaagca ggcccttgag
     9841 gcggttcgtg cggagcgcta ctccggcatg atgctggata tcggccttcc ttccatcgat
     9901 gggatggaag tattgagaca gatcagacaa accgatcagc agttgccaat catcatgatg
     9961 acagcatccg gtgcgaagga gtccgccgtc aaggccatcg gggtcggtgc gcaggcctac
    10021 ctgttgaaac cgttcgatgt cgatgaactc cagcagatcg tggagtcgtg gtttcgaacg
    10081 gtgtaatcca gacaatcgtg tgagatggag caaggaatga acgagggctt ggaatgcgat
    10141 cagcttgtga ccaacgataa ccaaacctgt ctcgtgcgac tgaaccgcct gagagagcta
    10201 gtcaaaattg ctttgatgat cggcatcgtc tccttatggt ccgggacatt tccggatcag
    10261 acgtcagctc aagtgccacg cattcagggg caggggaccg cggcgtccgg aatgggcaat
    10321 gccttctcgg cccaggcgga tgatccctcg gcgctgcact acaatcccgc cgggatgaca
    10381 caattgtccg gcgttcagtt catggcggga tttctgttcg cgggagggac gacgaacttc
    10441 accagttcca accctccgac ggcgggggtg aatgccacag gtgatcgcaa cggcagtctg
    10501 gcttggccgc cacccgggca tacgtacctg acggcgaatc tcaaggatct tggcgtgacc
    10561 gcgttgggcg acctgaccgt gggcatcggt gtgacggtgc cgtttggatc attgactagg
    10621 tggcctgatg acggaccctt ccggaatacc gtcacgttca ataccctgcc gcttctcgat
    10681 atcaaaccga cggtggccta tcaattgacg aacgatctgt cggtcggttt aggagcagac
    10741 atctatacgt tcagcggatt attcggtgaa ggccacgtcg aacagcggtt tatttctccc
    10801 ggcgggccgg cgacccctct gttcggggcg gcggggtcca aggttgaact caacggaaaa
    10861 gatacggcgg ctggtttcaa cgcgagcgtt ctgtacacgg cgctccggaa cggtgacgga
    10921 aaacccacgg caaatatcgg attcgtgtac cgcagtcagg ccaccctcca tctttccggg
    10981 gcgctccttt cgaatggggc aaaggtgcag gatgcgaccg ctactctggt gctgccgcag
    11041 gtcatgaccg gggcaatcgc cctctggccg gttcggacga acgagcggga atggaagatt
    11101 gaactggatg tggactacgt cggctggaaa tccgtgcgaa atctcgatgt ccatctagcc
    11161 aacggcacga cgatcccgca gccgcaaaat tggcacagca cgtacggcgt catggtcggt
    11221 acggagtaca agtggatgaa ccttgagtcc atgccaggat gggaagtggc tgcacgcgcc
    11281 ggctatacga atcagcagaa tcaaatgccg gatctctcgt tcaatcccgg cattccctca
    11341 gctgatgtga acatcatttc gaccggcttg gggctcttgt gcaaggagaa cgggtcgttc
    11401 ttcggcctga cgcaatgcgg caatttagga atcggcgttt tgaaaccaaa aggaatcggg
    11461 atcgatctgt cttaccaggc cggcctgtac gagcaacgga ctgtcagtgg caacaccggt
    11521 ttgcgggcgg gtgtgaacgg cgtgtatagc agcacctacc acacgggcgg gctgtccgtt
    11581 cgcgcgatgt tctgatgcag ccgtgatgac tcgtcgggta aggtgcgtgt gcggtctagc
    11641 cttcgtgata gacgcgaggg atacgaggcc cgatggcgca gaggatttcg tagggaatcg
    11701 ttccggacca ttcggcgagg tcgtcggccc agatggcttc agctccctgc tcaccgatga
    11761 gggtcacttc atcgcccacc tgaacggcac caacgtcggt cacatccacc atggtcatgt
    11821 ccatgcagac aagaccgacg acgggagcac accgcccctt gatgagcacc aatgccttat
    11881 tggacaaccg tctgctgtag ccgtcggcat aaccgatcgg caggacggcg attcttgtct
    11941 ttctgcttgc gacgaacgtg cggttgtagc tgacgctgtc gcccggttgg attgtgcgca
    12001 gttgcatcac cgtcgtccga agagacagga ccggcttgag tgtcggggcg gggatgttgt
    12061 tcgggagcgt gtgatagccg tagagcatga tgcccggtcg caccagtgaa aagtgtgcct
    12121 gaggaaatcg gacgatggcc gcgctgttag cggcatggat ggccgggatg atcagcccgt
    12181 gcttgcgcac ttggtcgagc atctttcgaa acgattcgat ctggcgttcc gtgtgggccg
    12241 ggtcgttgcc gtcgccatca gccaggtgag tcataaatcc ttcgacgcgg agggatttcc
    12301 acgagaggag cgcatcgagc aatgtcgcca ggtcttctgg gggaaacccc agccggctca
    12361 ttcccgtatc gactttgatg tgaatcggga gcgagacctg ttttgcttcg gccgccttgg
    12421 caagtgccgg gagcaggcgc tgatcggtga cgacgggggt caaacgacgg gtcacgagtt
    12481 cctcgacatg gtcgtcgaac atgtcgacca ggacgaggat ctcagccgtg atgccggctt
    12541 cgcgcaaggc gagaccttcc tggacggacg ccactgcgag ccgtgaaatc ccttgccgga
    12601 tcagtgttcg ggagatatcg accgcgccat ggccgtaggc attagccttg acgacggcga
    12661 tgatgccaca gccgcccgaa aggcgccctt ggatctgcga aagattatgg ctgagtgcgg
    12721 cgagactgat ggtggcgtag gtcgtgtgca aggtcagcag gccgatgaaa aagtccgcca
    12781 gcgttgttct cgcgtcgctc agaggctcaa cgtacggcag aaagtacgcg tcgcctcttc
    12841 gctcgctgcg gccgtgctgg acggccattt tgatcagcct gcaccggata ctgaattctt
    12901 cggtggcggc tgcttgtctt actcagagag cacggtgaat caacgggacc cggcgatcac
    12961 acttcgagaa tttcctgctc ttttttcttg atggcctcat cgatcttctg cacgtactgg
    13021 tcggtgagct tctggatttc gacctctgat ttccgtaaat cgtcttcagt caatttcgca
    13081 tctttttgga gtttcttcaa ctcttcgttg gcatcccgac ggaatccgcg gatgtgcact
    13141 ttggtgtctt cgccgtgctt cttgcaaatc ttgctgagct ccttgcggcg ttcttcggtg
    13201 aggggaggga gcggcacgcg aatgattttt ccatcgttcg acggcgtgat gccgagtcct
    13261 gaggtggcca aggccttttc aatctccttg atgagttgcg gctcccacgg ctggatggtg
    13321 atcaatcgcg cttcgggcgt ggagagattg gccacttgcc tcaacggcgt catggtcccg
    13381 taatagtcca cgcgaacgtt atccagaagg gcaaccgatg cgcggcccgt tcggagaccg
    13441 gcgaggtccc gcttgagatg ttccaacgcc ccttccatct tccctgtcac ggcttgacga
    13501 attgcagcag ggttcgacat agccactcca ctagatgtta gcgctgatcc gcggtgacga
    13561 tcgtgccgat cggttctccc gaggccacgc gcttgaagtt tcccggcact ttgagattaa
    13621 agacgaccaa cggcaggcgg ttgtccatac agaggctgat cgccgtggcg tccatgactt
    13681 tcaggtcctg gttgaggatc gagaggaacg agatttctgt gtattttttg gccgacggat
    13741 tcttcacggg gtcggcgtca tagatgccgt cgactttggt gcccttcatg atgacctggg
    13801 ccccgatttc catcgctctg agcgcggcgg cggtgtcggt cgagaagtac gggttgccgg
    13861 tgccgccggc aaaaatcacg acccgtttct tttcgaggtg ccgaattgcg cgccggcgga
    13921 tgtagccctc ggccaactgg cgcatttcaa tggccgattg gacccgggtc atgatcccgg
    13981 ttcgttccag ggtgttctga agggccaacg cgttcatgac ggtagccagc atacccatgt
    14041 aatcggccga tgcccgctcc attcccgacg cgctggcagc gactccgcgg aaaatgttgc
    14101 cacctccgat gacgacggca acctcgacat cgagagcgac cacggccgca atttcaccgg
    14161 cgagaccttc gaggacggag ggttggatgc catatccctg ctcaccggcc aacatctctc
    14221 cgctgacttt caggaggatg cgccgatatt tggcagagct catgcttcgc ccagttggta
    14281 gcgtgtaaag cgacggatgt tcatgttctc gccgatcttg gcaatcttct gcgagagaag
    14341 atccttgacc atgacggcgg ggtccttaat gaacgcctgc tccatcaagc agctttcctg
    14401 gtagaacttt tccaatttgc cttcgacgat cttcggccag gccgcgggag gcttgcccat
    14461 ttccttggct tggccctcgt agatcgcccg ctctttgtcg acgagctctt tggggatgtc
    14521 ctcgcgcttg acatagaggg gcttcgccgc tgcgacttgg agcgccagat cgttgacgaa
    14581 ggctttgaac tcttcgttgc gagccacgaa gtcggtctcg cagttcactt cgatcaagac
    14641 gccgatcttc ccgcccatgt ggatgtaggc gtggatcagc ccctggttcg tctcgcggcc
    14701 ggatttcttc tgggccgcgg cgaggccttt ttgccggagg taatcaacgg ccttctcgat
    14761 gtcgtctccg gtctcttgga gagccttctg acaatccaga atgccggcgc cggttttttc
    14821 tctcaattct ttgactaatt gacttgctcc agccatacgt aaccgtcctt tttcgtcact
    14881 cttgatgaat ggggtgcctt ggtgcgaaac gatgtctatg acgcgggtgc cgccgccagc
    14941 gatggtgcga cacctttgtc gaccgcggca gggctcgctt ggaactcggc ctcgtcgcgt
    15001 tgcgcgcgca aatgggcgcc ctcgatacac gcatcggcga tgcgggacgt aatcagcttg
    15061 attgaacgaa tcgcgtcgtc gtttcctggg attggatagg taatgtggtc gggatcgcag
    15121 ttgctgtcga tgatcgcgat gacagggatg tcgagacgcg tggcctcctg gaccgcaatg
    15181 cgctcaattc ttgtgtcgag gatgaacacg gcgccgggaa ggccgcgcag gttcttgatg
    15241 ccggtgagga acttctgaag tttgacgatc tctttctgct tctgcgagat ctcttttttc
    15301 gtcaggccgt gttgcgccgg atccgccaac atcccttcga gcttcttcat cttgtcgatg
    15361 cttcgccgga tcgtctgaaa gttcgtgagc ataccgccca gccaccgctg gttcacgtaa
    15421 aacatgttgg cgcgcacggc ttcttcctga agaatttccg cggcttggcg cttggttccc
    15481 acgaatagga ccgactgccc tgaggccacg gtgtcacgga cgaaggcata ggcctgttcc
    15541 atccgcgcaa cagtctgctg caggtcgatg atgtagatgc cattgcgttc gccgaagaga
    15601 aacttcttca tcttcgggtt ccaccgattc gtctggtggc cgaaatggac gcccgcttcc
    15661 aacagctcct tgattgctac tactcccatg ccttcacctc ccctcaaggc ctgcagagcg
    15721 cccgattcac acaggcgagg gagcatgccc ccttattctc aagccaagcg acgtcatcag
    15781 acgtcgaagt ttatttgaat actattcctg ctccgctcaa ccgcggagaa ggaagtctgt
    15841 ctagtcaacc caaaagcctt ggaacgttag catactgtcc ggtcgtttac aaggttcaaa
    15901 tcgagcaaat tgtctctagg atcgataact tgcgttaata cggatgtaat cataggacaa
    15961 atcggtcgtc catatatacg cctggtgtcg tccctggcca aggtcgactg cgatggtgaa
    16021 ctccttatgt cgaaagacct tcgcgatccg ctgctctgca accagcccga gcccaacccc
    16081 gtgccggacc atgggcacgc ctccgaaccg cacgttgatg gtggaaggat tcagaggaac
    16141 tcccgcacgg ccgatggctg ccatgacccg gccccaattg gcatcctcgc cgaagagcgc
    16201 ggttttcacc aaattagatg ttccgatgct ctgtgcgacc tgtctggcct gctggacggt
    16261 cctcgctccg gtgacttcga tcttgacgac tttggtcaca ccctcaccgt cgcggcaaat
    16321 ggccagtgcc agaggctggc aggcttccgt taagaggtga acgaattgac ggaacgccgg
    16381 ggtaccttcc ttgatgattc ggttgttggc catgccgttc gcgaggcaaa gcaccgtgtc
    16441 gttggtgctg gtatctccgt cgaccgagat gcagttgaac gattgcccga ccgctgcagt
    16501 caaggcttgc tgcaatgctt ttcttgtaat ggcggcgtca gtcgtgaggt agcccaacat
    16561 ggtggccatg ttgggatgga tcataccgga tcccttcgcc atgcctccga tcgtgacgac
    16621 tcgtccgttg attttctgtc ggcgcacgac ggtcttcggc ttgagatcgg ttgtcatgat
    16681 cgcacgagca gcctgcattc ccgcacggct gctgagattt tttaacaacg cggggaaggc
    16741 cttcacgatt cgatcaagcg gcaaggcgcg accgatcaca ccggttgagc caacgaatac
    16801 ttcgtgcaca gggatgcaga gcgctcgcgc gacgagcgcc gccgttttct ctgccgcctt
    16861 gagacccgac gctcccgtgc aggcattggc atttccacta ttgacgagga tcgcgcgccc
    16921 cattccgcgt cgaaggtggc ggcgatcgag aatcaccggt gccgccacaa cctgattgtt
    16981 tgtaaagacg ccggcaatag ggccgctgcg ctccgacacg accagtgcca gatcgaggag
    17041 gcctggtttc ttgatgccgc aatgaaggcc ggaagcgaga aagccttgcg gggctgtgac
    17101 tccactgctg ttgagtgtgg tcattcggat ctatgcatca caagggttgt cgtgctgtta
    17161 ggggtatgcg gcaggagcag tcaatcctgt ttcttcaggc agtccaagca tgaggttcat
    17221 ggcttgaatc gcctgacctg ccgcaccctt gaccaagttg tcgacggcgg cgacagtcac
    17281 gatccagccg gcgcgctgat cgacgtagac gccgatgtcg caatagtttg agcctttgat
    17341 atagcgggga ttgggcacca cctcttcgga gagcctgacg aaacgttcgt tcttgtaaaa
    17401 cgttctgtac agtgcgcgaa tgtctgggag cgccatcggt tgtttcaagc ggcaataggc
    17461 cgtgctcaag atgccgcggt tcattggaac cagatgtggg gtaaaggtga cggtcgatga
    17521 cacagggtca atggttgatg atccggtatg gcgttgaaga aggccgccga gttcctgctc
    17581 gatttcggga atatggcgat gctgtccgat cttgtaaggc tccagcgatt catgtgcttc
    17641 cgggaaatga taggggagtg cgggactgcg tcctgcgcca gaaatgccag acttggcgtc
    17701 gatgacgacg gtctgagcct gcaccatatt atgggcgaac agcggagcca gttgtaggat
    17761 ggcggccgtg ggatagcagc ccggtgaggc caccagcttg gatttcgcaa tctcggcgcg
    17821 gtgcagctcc ggcagtccgt agaccgcgtc cttcaacagg tgcgcgtgcg tatggggagt
    17881 ttgataccag cgttcgtagg cagccggatc tttgaggcgg taatccgcgc ttaagtcgac
    17941 gacgaatttg ccagttctca tacaggccga cactggggcc agtgacttag tgtgggggag
    18001 ggccagaaac accgcatcgg cctgttcggc aatggcctcc ggcgcgagtg cttgaaagga
    18061 gagcgagacc aggccggcca aactcgggaa tatgctcgcc accgggctgc cggcggattt
    18121 ttccgaggtg accgcgacta gctcgaaata cggatggagg gcggcgagac ggacgagttc
    18181 cgctccggca tatccactcg cccctgcaat ggcgacacga atttttctca tcatgctacc
    18241 ttcgcgacag ccctcaatct tatagctgca gacataaaaa aagggaaggc cgggagcctt
    18301 cccttttctt cattctggct ccggcgctta gcgcttcgag tattggaacc gctttctggc
    18361 gcccttctga ccgtatttct ttcgctcttt cacgcgagag tcccgcgtca acagtccctc
    18421 tttcttgagg ggcgcgcgga gcggctcgtt caggctgacc agcgctctgg caatggcatg
    18481 ccgcaacgcg ccggcctgtc cggtcggtcc gccgccgtgc acggtcgcct tgatcgaaaa
    18541 cttgccggcc acgcctgcga gttcgagcgg gagttggatg atctgccgaa gggtcagacg
    18601 gggaaacgcc ttttcaagcg gttgatcgtt gatcgtgatg tcgcccgccg ttccggtcac
    18661 ccaggctctg gcgatcgcac atttccgtcg tccggttgca tattgagttg ccactgccat
    18721 gctagaacgc ctccttcttc ggtcgtctcg acaattagag tgtaatcgtt tcagggtttt
    18781 gggcctggtg cggatgcgcg gagcccacgt acacgcgaag ttttttggcc atatgattgc
    18841 cgagcggtgt cttcggcagc atgccttcga ttgctgcaac cagtaattgc gtcgggtctt
    18901 tgcggaagac gtgcttggcg gtcgcagttt tcaatccgcc cggataaccg gaatggctcc
    18961 ggtagagttt ggtctccatt ttgtcaccgg tcagttgaat tttttcagcg ttcacaatca
    19021 cgacatggtc gcccatgtcg acgttcggcg taaaggttgg gcgatgcttg ccgcgaagca
    19081 cccccgccac ccgggccgcg aggcgtccca aggtcttgcc ttcggcattg accaggtacc
    19141 atttttcttg cacgtcagct ggtttctgta gataagtcga catcgtctct ctgcgctcct
    19201 tcacttgcga atgggtcaag gttcctgaac ttcctgcttg atgttcttac tgtccaactt
    19261 ggccagccat gagtcgaggt tggccgtact ccgtcgcgcc aacacgtcct gagtgatgta
    19321 aatcggtgcg tgcgtgcgca gtgccaacga gagggcgtcg ctcggcctgg cgtcgaccgc
    19381 gcgctccacg ccgtgattct cgagatgcac catcgcgtaa tatgtgctgt tcttgacgtc
    19441 ggcaatcacg atacggcgga cggtgatccc cagatggtcg atgaaactct tgatgaggtc
    19501 gtggctcatc gggcggggag taatagtggt gtcaagggcg agtttaatcg catgcccctc
    19561 tgctgcgccg acccaaatcg gaaaggaatc ctgctggccg tcatgccgag ccaggacgac
    19621 gatcttcgtg tccgtgttgg tttcgtcgag cacccgatcg actttgtatt cgatgagtgc
    19681 ttgatccttt gttgaaccgg agtggcctat gtcgtccatc gtctttacga gtccaatttg
    19741 ccgaagtctt ccggcttcaa attttccagc cactgttcga gttcttcgga gctttgtttg
    19801 ttgagcacgg attctttggc gaaaatcgga gcattcgacc gcagtgccag cgccaccgca
    19861 tcgctcgggc gcgcgtcgac ggtgtattct gaatcttcgt acataagatg gatcttggca
    19921 aagtaggtgt tctcgttgag gtcggtgatg accacgctga gcaccttggc atcgtacgca
    19981 tcgagaatag atttcataaa atcatgcgtc atcgggcgcg gaggcgcaac attttccaat
    20041 gccatgctga tggcgccggc ttccgccttg ccgacccaga tcggaagcat gtccgcgcct
    20101 tcttcgtcgc gcagcactac gatgtaggcg ttgttatagg ggtcgaacat caaccctttg
    20161 agatgcatct gcgtaatcac ggcaatccaa acccctcaac tcttgcggga acccgatcaa
    20221 taataatgaa caggaagagc acaatctatc atttttgaaa gttgcctgtc aacgaagcct
    20281 gcgagatcgc aggagaaatt cccgaacgac tcaacaagag gtgcggtgag gattgagaat
    20341 ccctggcggc tcaggcttgc ggtcgatagt tctgatcgag ttttgaggta tcggccggtt
    20401 ccccgccgat cttgaggaac gccttcatct gcttcttgac gagttcacgt ccgctctcat
    20461 ctcccagatt gacttggtat tcgttgatca ccatcacccg catggattcc cattttttcc
    20521 agcagggttt gcatactttc gacttgattt cagcctccag ctttcccatg aagagggtgt
    20581 cggtaatcgg ctcaccggct tgtccgcagg tgacacattg aacttcggcc atggtgaaaa
    20641 gatcctcctc gtaaaaagat gccaaccgga ggggattgta gcatccgcct tcgtgcaaag
    20701 aaaagagggg atccgattga ccgttgtcgg acttcatgtt agaaatggcg ccctttgggg
    20761 gatcactgcc cggatggcgg aactggcaga cgcgccggac tcaaaatccg gttctcgcaa
    20821 gagagtggga gttcgaccct ccctccgggc accaaaacca gggctagagg ctggacgttt
    20881 ctggtacggt ctcggctggt cgaccggcaa attaaccccc ttgacatgta ccggacacta
    20941 gcctatagtc cgccccctag catgatgaaa atacgaagat ttcatgagtc gataacgttc
    21001 ctttcttgtc gtccgtcatt tacttcattt tctaactcac caaggaggca gcacatgcgt
    21061 aacgtgttgg cgctaggagc ggcagtactt cttgtcggct ccatcggtgt ggcaggagct
    21121 caagagcgcc tgcagtccaa tcagtccggt atgggcgtgg gcactggggt tggcgatgtg
    21181 tctgggtcaa gcactcgcgt gaccacgttc gaccggagca acttcctcga tcggctgtcg
    21241 caggctgaaa cgatctatgg ccgtgtgctg gcgatcgata ttccagccaa gaagatgcac
    21301 ttggaaggtg gaggaagctc tcacgatgaa ggacgtgctg gcggcggtgc gatgaactat
    21361 cggacggtgt atttcgatga tcataccaat atggatcaaa ttcgcgtcat cgaaaccggg
    21421 gacgatgtaa cgatccaggc atttgaagaa acgactgcga gccagccgta tgggaccggg
    21481 aagaagcttg ttcgcgagat taccgttctt cgcggcaatg agaagctcgc cggcttcggc
    21541 gggcttggac agcggccgaa tccgatgacc gagcgcggta ttgaaacgaa cagcggcggg
    21601 atgaccggcg gtgtggccgg cggcgtgctc cccggcaaga tcgattccgg catcaccagc
    21661 tcagtcggtg aatacaccgg cgcagctccc tgctggcagt gcgaaccgca gccgggttgg
    21721 ggcaacaatg tcggcaacaa gtccgactac ggggccgatt acacgaagcc caatctcgtg
    21781 aagggtttga actaacgcgt aggcgacagg cctgtgcagg aaggggcagc cgaagaggct
    21841 gccccttttt ttttgctcat gatgttgccg ttgtattgcg gcacaggtgc cggcctggta
    21901 ggatttcttc gtggtcgtcc ggtgtggggg gtgttcctca gaagaagggg aggggtatga
    21961 gcggtcaggg tcaatcgaat cgtcccgatg cggagctaga tctgcgcggg gtgatctgtc
    22021 cctacaattt tgtgaaaacc aaactcaagc ttgaggcgat ggaggaaggg caaattcttt
    22081 ccgtgattct ggatgccggc gatccgattc gaaatgttcc ccaaagcgtc agcaacgaag
    22141 gccatacgat tctctctcaa gagccgatcg accattctta ccgtgtcgtc attcggcgcc
    22201 gcgccgacga gtagcatgtc gtgtagctcg aagccgtgtt ctcctcactc ccttcggcgg
    22261 ttctccttcc acgaccacgg tcacctcggc gttttcggca agggggcgtc cctgtgcaag
    22321 cagcgaggcc gcggttcctt ggcacaggcg ttcggtcgtt ctcgtcatgt ctgtcgcaag
    22381 cgtcacctgc ctgtcgggaa ggatacgatg aaggctctgt agaatatgcg cgcagtgtgc
    22441 ggacggtgca aaaaacacta acgttacgct gttccctttg aagtgcgcca ggaatctatt
    22501 tgccaggcgt ttggtccgcg ggaacttgcc ttcaaagtga acgcgatctc cggaatagcc
    22561 ggagattgcg acggccgctg ttaaggccga tggtcccggg atcacggtga ctggatagcc
    22621 tgcctgttgt gcggctgcaa tcagcagccg gccgggatca tagatcacgg gcataccgct
    22681 gtctgatacg agtgcgacat gctgtcctcg atgaagcgcg tggagaagga cggaaatttt
    22741 ttcgtcatga ttccgtgggc catagctggt aatcgttgcg ctgatgccgt ggtgatcaag
    22801 gagggcttgg gtcgccaggg gattttcggc ggcaacgatc gacacgtttt tgagggtgtt
    22861 gagtgcgcga atgctgaggt cgtcgaacga tccgatgggc gttcccacaa tgtacaaagt
    22921 gccggtgcgg cgagaggcag ggtcctgcca ctcgggatag attggttgac tcctctgagg
    22981 cgccatcgat ataattcgct tttcaagccc agtctgaatg tcctgaaaag ttcaggggat
    23041 agacctcatg gccgccgtgt ttttcatggt cacaatggcg ctatattttg ccgccacggt
    23101 gtcctttctt gccctcttgc tgcggcgtgc cgacgccctg tcaaccgttt cccttacgat
    23161 tacttccatc ggattcgtca cacataccat tgcgctggtc gcgaggatgg tcggggcgac
    23221 catggcggcc cagccgagca ttcatgaggc gctctctttt ttctcctgga tgctgattct
    23281 cgtctttctt gccgttgaat ttcgccatcg gcttcacgtg ttgggctctt ttatcgttcc
    23341 gctcgcgctg gtttccctcg tatctgctgc cgctctgccc gaaaccgtgc ccacgcttca
    23401 gccgatgttc cggaccctgt ggatccacgt cacgctcagt atgttaggga ccgtcggttt
    23461 tgccgtcgcc tttgttgctg gtgtcatgta tctgatccag gacggtcttc tcaagtccaa
    23521 acgcttcaat gtgctctatt cgaagctccc tgcgctggat tatctcgacg aattgaacca
    23581 gcagtcgatg gtgctcggat ttccgctgtt gacgcttggg atcatcaccg gagcgatctc
    23641 tgcggagttc gcccgcgggg cctacttgaa ctggaatccc gagcaaacct gggctctggt
    23701 gacctggctg ttttatttta tcgtgttaac gggccgcttg accgtgggat ggcgtgccaa
    23761 gcgggcagcc tacctgacga tcattggatt tgcctgtgtc atcctgacgt tggtggctgt
    23821 ggcggtaaaa agcttcgggt cggtgtcctg atatgcacat tgtcgttgta ggactcagcc
    23881 ataagacggc tcccgttgaa atccgggaga aattggccat tccggaaagc cgtatcggag
    23941 aagcgttgac gcggctctgt tcctattccg gagtgaagga agggatgctg ctctcgacct
    24001 gcaatcgcgt cgaagtctat gcggtggtcg aagatctgga atctgggtac agtcgcattc
    24061 aagaatttct cgccgatgcc catctctcgc tgtcctcgga gcagttaaca ccgcatctgt
    24121 actggcagag cggagatcgc gccattctcc atctgttccg tgtggcctcc agcctcgatt
    24181 cgatggttgt cggtgagtcc cagattttgg gccagctcaa agacgccttt gaaatggcct
    24241 tgacccataa gacgacaggt ctgattctca ataaagtggt gaagaaagcc atttccgtgg
    24301 cgaagcgggt tcggaccgac acgaagatcg ccgaaatggc ggtgtcggtc agctatgcag
    24361 ccgtcgaact cgccaagaag atcttcgccg atctcagcga taagaccgtc ttgctggtcg
    24421 gggcggggga aatggcgaaa ctcgcggcac ggcacttcat tgccaacggc gtgagtcacg
    24481 ttcgcgttac gaccaggacg ccgcagaacg cggttgaatt ggccgatcga ttcaatggca
    24541 cggcagttcc gttcgagcat tttcgagagg acatggctgc tgccgacatt gtccttgtct
    24601 cgaccggagc ttcgcattat ttggtcggca gcgacgacgt gcagcgggcg gtgaagcagc
    24661 ggatgaaccg cccgatcttc ttgatcgaca tctcggttcc gcgcaatatc gatcccgcag
    24721 tgcgccatgt cgataacgcc tttctattcg atatcgatga tttgaagagc cgggtcgagc
    24781 aaaatcgcgg tgaacggctc caggaagccg aaaaggccga gcatatggtt gtcgaggagg
    24841 ttagtgttgt tcggcaatgg ctgcagtcac tcgaagtcac tcccacgatc gtcgcgctga
    24901 agacccgcgt cgaggacatc aggcgagccg aggttgaaaa agtcttgggg cgtctgggga
    24961 atctttcgcc gcaagagcgg gcgttggtgg agagcatggc gtcgtccatc gtgaataagt
    25021 tgattcataa cacgatggtg acgctcaaga gtgaagtgac atcggctgat ggcgccgcgt
    25081 tcgtcgaggc ggcgcggaga ttctttaatt tgagcgactc ggggcctgca ttgctgaccg
    25141 aggaagttga gactgaaggg acaacccgga gatcgggcgg gaacgaaccg aactgaagcg
    25201 ggtcaattgc gagggagaag agatggggct ggcggccaaa ggtccgaagc tctttacctg
    25261 tcccaactgc cggaaagcct atctaggcta cgatccgctg ccagattgtc cggcttgcgg
    25321 gttcgattat cgggtgcggg cagggttccg gtgggatgtg ctggtctatc tgttgtcgat
    25381 actcggcttg atgagctact tcctcgtctc cacttcatat cgcagtttga ttacggagcc
    25441 gatttcgaac gagcgtggtg tctcagcggt atcgggtgaa gagaaactgc ccggcagccg
    25501 gactcttccg atgagctacc cagcagaacc gcgtgcgagt cattgaacgg agtgtatgac
    25561 ccattcgatc caagcacggt cacagttggt tcttggaaca cgcggcagca agctggcggt
    25621 gcaccagagc gagtgggttc aggctcggct caaagagctg gccccgcatg tcacggtgac
    25681 gctgcggcgg attcatacgg ccggcgacaa gattctcgac gtcccgctgg cgaagatcgg
    25741 cggcaaggga ttgttcgtca aggagatcga agaggcgctg ttgtccggcg aaatcgatct
    25801 ggctgtgcac agcatgaagg acgtgccgac tgagctgccc gacggattgg agttgttgtg
    25861 tgtcccgctc cgtgaagatc cgagggatgc gttgatcagc cgggacggac ggttgttcaa
    25921 agatcttcca cgtggagcga aggtcggaac gagcagcctt cgacgccagt cccagttact
    25981 gcaggccagg ccggatcttt ctatcagtat gttgcgaggc aacctggaca cgcgaatgag
    26041 gaagttgcgg gagggccagt tcgacgcgat tgtgcttgct gcggcggggt tgcgccgtct
    26101 ggggtgggag catgagatta ccgagtatct tgcgccggag atcagtttgc cggcgatcgg
    26161 tcagggagcc ttgggaatcg aaggtcggcg ggacgatggt ttcgtgcggt cgctcctcgc
    26221 cgggctcgaa catgctccga ccaggacgat ggtgttggca gaacgggcgt tgctccatcg
    26281 cttgcaaggg ggttgtcagg ttccgatcgc ggctcatgca acggtacatg gatcggatgt
    26341 cacattggaa ggcttggtgg ccagcgtcga tgggaaggaa gtcatccgcg agaccgtacg
    26401 ggggaccgtc gcggatcccg agtcgatggg aatccagttg gcagaacggc tgcttgcgcg
    26461 gggtggcgac agaattctcc aaacgattta cggaacgtcg tgatgggcac gactcgtcga
    26521 gggaaagttt atctggttgg cgcagggcct ggagatcccg gtctgttgac gctgaaaggc
    26581 aaggaatgcc ttgagcaggc tgatgtcgtc ctgtacgatt acctggccaa cccggtcttg
    26641 ctgcaacaca cgcgggagca ggccgagcgg atttatgtcg gtcgccgagg tcggggtcac
    26701 tatcaggacc aagcggatat caatcgtctg atgatcgaga gggccaaggc ggggcaggtg
    26761 gtggttcgct taaagggcgg ggatcccttc gtgtttgggc gaggcgggga agaggctgaa
    26821 gccgtggcgg ctgcgggagt cgacttcgaa gtggtaccgg gcgtgactgc cgccgtggcc
    26881 gttcccgcct atgccggcat tccagtcaca caccgtacgt tggcttcgac cgtgacgatc
    26941 gtaacgggtc atgaagatcc gtccaagcag acgacggtca tggactggcc gaaactggca
    27001 gcaacatctg gcaccttggt cttcatgatg ggtatgaaga cgcttccgac catcgtggcg
    27061 cggttgctgg aggaggggcg ctctccccag acgcctgtcg cggccattcg ttgggggacg
    27121 agagccgatc agcagacagt ggttgggact ttgaaggata tcgtggagaa ggctgaacag
    27181 gcgagattcg aaccgccaac cgtgatcgtc gtcggcgacg tcgtcgggtt gagggagcaa
    27241 ttgaactggt ttgaaaaacg accgctcttc ggcaaacgaa ttgtcctgac gcgggcccag
    27301 gagcaggcgc aggagttttc tcgcctattg gcagcttacg gagcggagac gatcgaagtg
    27361 ccgacgattc agattgtgcc gcctgtcagt tggcaggcta tcgatcatgc gatcgctcgc
    27421 ctggcaagct accagtggct gatgttcacg agcgtaaacg gagtgaagcc gttcatggag
    27481 cgtctccgtg cggcgcacaa ggactcacgt gcgctggccc atttgcaggt ctgtgcgatc
    27541 gggccccgta cggcagcgga gttggagcaa tacggcctga gacctgatat ggtccccttg
    27601 gagtatcaag ccgagggaat cctggcggtg ttcgaggggc aggatcttcg cgggaagcat
    27661 gtcctgattc cacgtgcgga agtggcgcgg gaattcttgc ccgaacaatt gcgtcggatg
    27721 ggggcggaag tcgacgtgat tccggtctac cgcacgaccg tccctgcggc tgatctgagt
    27781 cgggttcgtg agcagatcga agcgggcaag attgatcttg tcacctttac gagctcatcg
    27841 actgtcagga attttgtcga gttggtcggg ggatcggatg aggcgcggcg gttggggacc
    27901 aagacggcga tcggttgcat cggtccgatc acggcgcaaa cggcggcgga atatggattg
    27961 tcggtcgcca ttgtgccgcc ggaaaatacg gtgctggcat tggctcacgc catcgttcgg
    28021 catttcagcg aaggggctcg aatcgaagtg tctgcgttgc gatagcacgg ggcacacggg
    28081 tagcgtattg tattagcaaa gggaggatcg ttcatggttc cagtgaaatc attcatggtt
    28141 cccaaggaga aatttattac cgtggagcgt gatacggatg cccaaaccgc cgctcggatc
    28201 atgcgtgatc gcggaatcgg cagcttgttc gtgacgaacg gcaaggaaat tgtcgggatt
    28261 atcacggaca cggacatggt tcggcgtgtg gtggcgcccg gggtcgatac gcacaagacc
    28321 accgtcgagc agattatgtc tgctccgatc atgacgatcg aggataacaa gacgttgtta
    28381 gacgccaacg atctgatggc gcaaacgcat ctccgccatc tcggcgtgac gaaggatgga
    28441 aaactggtcg gaatgatttc cgtccgcgac ctggtcgtgt tcctgacgaa tttgccgagg
    28501 aagtaaccgt catggcattt ccgatccagc gtctgagacg gcttcgacaa cacgaagcgc
    28561 tgcgccgaat ggtgcgcgag acgagtctct caccgtccga tttgatctat ccgattttcg
    28621 ttggggaagg acgcgatcgg cgtgaggaga tcgcgtcgat gccgggccag ttcaggttgt
    28681 cgatcgatct cttggtgaag gaagcgggcg aggtggccgg tctgggcatt ccggcgatca
    28741 tattgttcgg gattcccgat cgcaaggacg aacggggatc atccggattc gaccccaatg
    28801 gaatcgtgca gcgggcaatc aaggccgtga aagatcaggt accgggcctc atggtcatca
    28861 ccgacgtctg catcgacgag tataccagcc atgggcattg cggcattgtc aaaaacggtc
    28921 gcattttgaa cgatgaaaca ctcgactgcc ttcgagcgat ggcacgcacg cacgcggagg
    28981 ctggtgccga tatggtggca ccatcggata tgatggatgg ccgggtggcc gcgatcagag
    29041 acgaactcga tcgagcgggc tatcccgaca tgcccattat ggcctatgcg gcgaaattcg
    29101 cttcctgttt ctacgcgccg ttcagagaag cagccgggtc gtcgccgcaa ttcggcgatc
    29161 gccagtccta tcaaatggat cctgccaata agcgtgaagc catgcgcgag attgcgcttg
    29221 atattgaaga gggcgccgac atcgtgatgg tgaagccggc catgccctac ctggacgtga
    29281 tccaccgggt gaaggcggag ttcgacctgc cggtggcagc gtatcaggtc agcggcgagt
    29341 actcagcttt ggttgctgcg caccggaacg gctggctgga tcgggaacgc acaattctcg
    29401 aatccttaac atgcatcaaa cgtgctgggg cagacatgat cattacgtat ttcgccaaag
    29461 atgctgcaaa gctggtggga tgaaagattc ggactaaccg caaagacgcg aagaagagag
    29521 tcaaaaaccc gcatctcaaa tatcctatgc ccaactacgg tgatgtctct ccccgttgca
    29581 aagaagaacc gcacctcgga caatttcatg
//
LOCUS       DATIFR010000188         4818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2422360, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000188 DATIFR010000000
VERSION     DATIFR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422360"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VLD60_10875"
     CDS             complement(<1..733)
                     /locus_tag="VLD60_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSB45508.1"
                     /translation="MADEHAHAAQQPQARENLIPDVKHVIAVSSGKGGVGKSTVAANL
                     ACALALTGAKVGLLDADLYGPNIPMMMGSTKGPEQQDGKILPVESYRVKLISMAFLVP
                     EEAPLVWRGPMVHQYLQAFFRDVLWGELDYLLIDLPPGTGDVQLSLSQMVPLAGAITV
                     TTPQEVALYDVRKGMAMFQKVNVPLLGIIENMSFFVCGHCGERTEIFSYGGGERAAEK
                     LGVPFLGRIPLDPAIRDGGDSGHPIV"
     gene            complement(878..1729)
                     /locus_tag="VLD60_10880"
     CDS             complement(878..1729)
                     /locus_tag="VLD60_10880"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSB45509.1"
                     /translation="MPDAVGTTQFAYKTCILFCLVAAATLIGTPLFGFLYGYTMFDWI
                     VFGILYVFTGMGITVGYHRLISHRSFECHPLVKSVLLIAGGWALQNSALRWCSDHIRH
                     HARTDQEEDPYNAQKGFWYSHCGWLFFNTTHRTENYERQLRKDPMIMWQHRHYHAIVI
                     SGLALPLLLGFLHGGVMGGISCFFLAGMFRMFMVLNSTFAINSLCHMYGSQPHGTHDS
                     SRDSWLVSLLTFGEGYHNYHHTYARDYRNGPEWYHFDPSKWIIYTLSLLGVATNLRRL
                     DSTVFSE"
     gene            complement(1873..2724)
                     /locus_tag="VLD60_10885"
     CDS             complement(1873..2724)
                     /locus_tag="VLD60_10885"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HSB45510.1"
                     /translation="MADQSADEQFSLHNVILFFAVAAATLVGIPAYWYCYGFNLFDWT
                     LFAVMYIVTGMGITVGYHRLVAHQSFECRNWVKGCLLVIGGWALQNSALLWCADHIRH
                     HAKTDQEEDPYNVTKGFWHSHCGWLFYETPHRTEKYEIRLRRDPIVLWQHRYYWPIVI
                     SGLCLPVMLGFWHGGAQGAVSAFLMGGLFRMFMVLNSTFTINSLCHMFGAQPHGTQDS
                     SRDSWLISFISFGEGYHNYHHTYARDFRNGPKWYNFDPSKWIIFTLALFGLASNLRRL
                     DPTVSAD"
     gene            2884..>4818
                     /locus_tag="VLD60_10890"
     CDS             2884..>4818
                     /locus_tag="VLD60_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSB45511.1"
                     /translation="MTHASSGLEPAVRRIGEELARLSAGRAPTLFERRWWSQAAINLA
                     MHDIAFKTQLFRFIDMLPSVTDDEHVVALADEYFGSMGNEIFGLRWGLRALNATGIGA
                     RLTGHAIRSQVEQMARTFIAGASIGEAAPVLGDLWNSGKAWSVDLLGEATTSDREADQ
                     YRDRCLDAMNALVQAARQWDSKPHLEEDHLGPIPRVQLSLKVSALSPHLDPIDPEGSY
                     HSVAARLRPIIDLAMQLPVSLIFDMEQAETKDLLIDIFTRLFSEPAYGTFAHAGLAMQ
                     AYHRATARDIEGLVEWARGRGVPITIRLVKGAYWDSDTIRYRQRGWPVPLFEQKADTD
                     ASYEALVHRLLAAASVIRPAFGTHNLRTLAYIEAVADELGLTPATWEYQMIFGMAEPF
                     QQAVTQRGRRLRLYTPVGNLLPGMAYLVRRLLENTSNESFLRKEYAESQPLSTLLSPS
                     AGNAASGPSPSAAPTEFRNEPLRDFSLAEAQAAMREAIAAVKRQVGRQWPSSARGLSL
                     TGPLMASHNPSRPDEVVGLVQGAAPADVERAINRAVERWHAWRETPAARRVEILRAAA
                     ALMRQRRETLAAWEVLECGKPWREADADVAEAIDFLEFYAAEWLRLALPQRLGHEPGE
                     LNHRLFHPRGVTAVISPWNFP"
BASE COUNT         1064 a         1356 c         1403 g          995 t
ORIGIN      
        1 ccacgatggg atggccgctg tcgccgccat cgcgaatcgc gggatcgagc gggatacgcc
       61 caagaaacgg cacgccaagt ttctccgctg cccgctcccc tcctccgtag gagaaaattt
      121 ccgttcgttc gccgcaatgg ccacacacga agaagctcat gttctcaatg atgccaagga
      181 gtggcacatt gactttttga aacatcgcca tgcccttgcg gacatcgtag agtgcgactt
      241 cctgcggggt cgtcacggta atggcgccgg ccagtggcac catttgcgag agagacagtt
      301 gaacatcgcc ggtccccgga ggcagatcaa tcaacaaata atccagttcg ccccacagga
      361 cgtcgcggaa gaatgcttgc aaatattgat gcaccatcgg cccgcgccag accagcggcg
      421 cttcctccgg aaccaggaac gccatggaaa tgagtttcac cctgtagctt tcaaccggca
      481 ggatcttgcc gtcctgctgc tcgggtccct tagtgctccc catcatcatg ggaatattgg
      541 gaccgtagag atcggcatcg agcagcccga ccttggcgcc agtcaatgcg agcgcgcagg
      601 ccaggtttgc cgccacggtc gattttccga cgccgccttt tccgctgctg acggcaatga
      661 cgtgtttcac atctggaatc agattctctc ttgcttgggg ttgttgggcg gcatgggcat
      721 gttcatcggc catggagtga tcctcctctg cagagatcgg cgcctgactt ctcaaaatga
      781 gaagcggtcg ttgatccatc ataagcgatg gacgagggga aagaaaatgg gccggttggc
      841 ccgccggaca tagctcaatt ggggtatctg ggagccgtta ttcagaaaag acggtcgagt
      901 cgagcctgcg aagattggtg gcaacaccca acagcgagag cgtgtaaatg atccacttcg
      961 aaggatcgaa gtggtaccat tccgggccgt tgcggtaatc gcgcgcatag gtgtggtggt
     1021 agttgtgata gccctccccg aaggtgagga gagagaccag ccagctgtcc cggctgctgt
     1081 catgagttcc atgaggctgc gatccgtaca tatggcagag ggaattgatt gcaaaggtcg
     1141 aattgagcac catgaacatc cggaacattc cggccaggaa aaagcagctg atcccgccca
     1201 tgacgcctcc gtgcaagaag ccgagtagca gcgggagggc caaaccggaa atcacgatgg
     1261 catggtagtg tcggtgctgc cacatgatca tcggatcttt gcgcagctgg cgttcataat
     1321 tttcagtccg gtgcgtcgtg ttgaaaaaca accagccgca gtggctgtac cagaaccctt
     1381 tctgggcgtt gtagggatct tcttcctgat cggtccgtgc gtgatgccgg atgtgatcgg
     1441 aacaccagcg caacgcggaa ttctgcaatg cccatccgcc tgcgatcagc aacacggact
     1501 tcaccaaggg atggcactca aagctgcggt gcgagatcag ccggtgatag ccgaccgtga
     1561 tgcccatgcc ggtgaagaca tagagtatgc cgaagacgat ccagtcaaac attgtgtatc
     1621 cgtacaaaaa cccaaacagt ggcgtgccaa tcagcgtggc ggcagcgacg aggcagaaca
     1681 aaatacacgt tttataggca aactgcgtag tcccgacggc gtctggcatg cctactccct
     1741 atgctggtgg atacgagact tcacacaatc tggccgggat cgagaagtcg cccagctctg
     1801 tatgtacaga agtagaggaa taagatcaag aaaatccttc caggcgccgc cgggaggaac
     1861 ggggcaagcc tgctagtccg ccgaaacggt ggggtcgagt cggcgaaggt tcgaggcgag
     1921 gccgaagagc gcaagcgtaa agataatcca cttcgacggg tcgaagttgt accacttggg
     1981 tccgttccgg aaatcgcggg cgtaggtatg gtgatagtta tgatagcctt cgccgaaact
     2041 gataaaagag atcagccagc tatcgcggct gctgtcctgg gtgccgtgcg gctgagcgcc
     2101 gaacatgtga cagagtgaat tgatggtaaa cgtcgagttc aacaccataa acatccggaa
     2161 aagcccgccc atcagaaacg cgctgacggc gccttgcgcg ccaccgtgcc aaaaccccaa
     2221 catgaccggc aggcataggc ctgagatcac aatcggccag tagtagcgat gctgccataa
     2281 tacgatcgga tcccggcgca gtctgatttc gtatttctcc gtgcgatgcg gtgtttcata
     2341 aaagagccag ccgcagtgac tgtgccagaa tcccttggtc acattgtagg gatcttcttc
     2401 ctgatcggtc ttggcatgat ggcgaatgtg gtcggcgcac cagagcagcg cggaattctg
     2461 cagcgcccat cccccgatga ccagcaggca gcctttgacc cagttccggc actcgaagct
     2521 ttggtgagca accagacgat gatagcctac cgtgatgccc atccctgtga cgatgtacat
     2581 gacggcgaaa agagtccagt cgaagaggtt gaatccatag caataccagt aggcgggaat
     2641 gccgacgagt gtggcagccg ccacggcgaa aaagagaatg acgttatgta acgagaactg
     2701 ttcgtcggcg gactgatctg ccattctttc tccttgtgcg acagagtgcg tttcatcgaa
     2761 gcacctcgag gccagaacga gacaagcggg cgacaatatg ccaaatataa gccatttatt
     2821 caacagtatc tttgcggtgt atactaaaat tgcttgggca ttttattggg agcgtaggcc
     2881 gctatgacgc acgcctcctc cggtcttgaa ccggctgtgc gccggattgg tgaagaactc
     2941 gcccggcttt ccgcgggccg tgccccgacc ctgttcgaac gtcgttggtg gtcgcaggcc
     3001 gccatcaatc tcgcgatgca cgatatcgcg ttcaaaacac agctattccg ctttatcgat
     3061 atgctgccgt ctgtgaccga cgacgagcac gtcgtcgcac tggccgatga atatttcgga
     3121 tcgatgggca acgagatttt cgggcttcgc tggggcctga gggcactcaa tgcgaccgga
     3181 attggcgctc gcctcacagg tcatgccatc cgatcgcagg tcgagcagat ggcgcgcacc
     3241 ttcatcgccg gtgcctctat cggcgaagcc gcaccggtct tgggcgacct ctggaatagc
     3301 ggcaaggcct ggtcggtcga tctcctgggg gaggcgacga ctagcgaccg ggaagccgac
     3361 caatatcgtg accgttgtct cgacgcgatg aacgcgcttg tacaggccgc acggcagtgg
     3421 gattccaaac cgcacctcga agaggaccat ctggggccga tcccgcgcgt ccagctttct
     3481 ttgaaagtct cggccctctc ccctcacctc gatccgattg atccggaagg tagttatcac
     3541 tccgtggctg ctcgcctcag accgatcatc gatctggcca tgcagttgcc cgtatcgctt
     3601 atcttcgata tggaacaggc ggagaccaag gacctgttga tcgacatttt taccagactc
     3661 ttttctgaac cagcctatgg cacatttgcc catgcaggtt tggcgatgca ggcctaccat
     3721 cgtgccactg cacgcgatat cgaaggcctg gtcgagtggg cgcgtgggcg cggggtaccc
     3781 atcacgatca ggctcgtcaa aggcgcctac tgggactcag ataccatccg ttatcgccaa
     3841 cgtggctggc ctgttcccct attcgaacag aaggcggaca ctgatgcgag ctacgaagcg
     3901 ctggtgcatc ggctacttgc ggcggcttcg gtcattcgtc ccgccttcgg gactcacaac
     3961 ctcagaacac tcgcctatat cgaagcggtt gccgacgaac ttgggctcac gccggctaca
     4021 tgggaatatc aaatgatctt cgggatggcc gaaccattcc agcaagccgt cacgcagcgc
     4081 ggacgccgac tccggcttta cacccctgtg ggaaacttgc tgcccggtat ggcgtatctt
     4141 gtccgccgct tgctggaaaa cacttcgaac gagtcgtttc tacggaaaga atatgcagaa
     4201 tcccaaccct tgagtacgtt gttgtcgcct tcagccggta atgctgcctc tggcccttcc
     4261 ccgtcggctg caccgacgga gtttcgcaat gagccgctgc gggacttttc gctagccgaa
     4321 gctcaagctg caatgaggga ggccattgcg gccgtcaaac ggcaggtagg ccggcaatgg
     4381 ccttcatctg ccagggggct ttcactgacc ggccctctca tggcttcgca caatcccagc
     4441 cgccccgatg aagtcgtcgg tctggttcag ggagccgctc cagccgatgt cgaacgagcg
     4501 atcaatcgtg cggtagaaag atggcacgca tggcgtgaaa cgccagccgc acggcgagtt
     4561 gagatattgc gtgcagcggc tgctttaatg cgtcaacggc gcgaaacgct tgccgcttgg
     4621 gaggtattgg aatgtggcaa accctggcgg gaggccgatg ccgatgttgc cgaggcgatc
     4681 gattttctgg agttctacgc cgcagaatgg ctcaggttag cacttcctca gaggctgggg
     4741 catgaaccgg gcgaattgaa ccatcggcta tttcacccgc gtggggtgac cgcggtgatc
     4801 tcaccttgga actttcca
//
LOCUS       DATIFR010000189         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2806979, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000189 DATIFR010000000
VERSION     DATIFR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806979"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VLD60_10895"
     CDS             <1..1021
                     /locus_tag="VLD60_10895"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HSB45512.1"
                     /translation="LQDQIEKQLLRFDLLSDELIELQEREEESKYELVINRELILPPY
                     SPTVLPWSEEREDQRRFRKSLLVSLLVALCFGILMVLWKLPLRDTADVTEIPERLVKL
                     VQRQMPKPPEPKPLEKKKPDDETKPKQKTETQQARAKAEQSGLLAFKSSLADLMDDAA
                     VAKLGADARVTNSGQQATGQTQRSLVVAQAREGSGGINTTTLSRNGGGTGKKLGGVQF
                     TRVESSVGAGMNDADRPLSSGPGPSRTDEEIQIVFDRYKAALYRIYNRELRTDPTLRG
                     KMVLRMTIEPNGEVSACTIASSDLASDVLKKEVVERVLRFNFGPKDNVSRITILYPID
                     FLPAS"
     gene            1684..>2622
                     /locus_tag="VLD60_10900"
     CDS             1684..>2622
                     /locus_tag="VLD60_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45513.1"
                     /translation="MLAFVLLLGAAFAAHAGTDFCSGYPLVNGYHVIDGNDPTLTPLT
                     LPSSIGIDANCVFQNFPISAKWPQGLTSTLNFKFDGYLAIFNNVYYTGNMACATTTTK
                     IWFVNPGAIYDPNNSCQDLFIPVEKIDKQSPAPYATIGVPFTYKLTIPVMYDPSTGTV
                     IGASGSANDLHSITVTDDLNATGVDLTYLGYVAYWKGSGTSVPLTFSNAGGVLSFSNF
                     PIIPAGQQIVIEITVVLNDTPTNVAGKVFTNTAAWSFGRLIDGTFYSPLPGQNGISQP
                     MTIAEPNLVVNKRSTATALNLGVMAPFTIDVQNTGGS"
BASE COUNT          599 a          731 c          774 g          518 t
ORIGIN      
        1 actgcaggat cagatcgaaa aacaacttct caggttcgat ctgctgagcg atgagctgat
       61 tgagctgcag gagcgggaag aggaaagcaa gtatgaatta gttatcaatc gcgagctcat
      121 cctgccgccg tatagtccga cggtcttgcc ctggtccgag gaacgcgaag accagcgacg
      181 gtttcgcaag tcgctgctcg tctcgttgct cgtggcgctg tgcttcggaa ttctgatggt
      241 cctatggaag ctgccgctcc gggacacggc cgacgtgact gagatacccg agaggctcgt
      301 caagctggtt cagcgccaga tgcccaagcc gcccgagccg aagccgctcg agaagaagaa
      361 gcccgacgac gaaacgaagc ccaaacagaa gaccgagacg caacaggccc gtgccaaggc
      421 ggagcagtcg ggactcctgg cctttaagag ctcactcgcg gatttgatgg acgatgccgc
      481 cgtggcgaag ctcggggcgg atgcgcgggt caccaatagt ggtcagcagg cgaccgggca
      541 gacgcagcgt tccctggtag tcgcccaggc ccgggaagga agcggcggta tcaacacgac
      601 aactctaagc cgcaatgggg gcggtacggg caagaaattg ggaggcgtac agttcacacg
      661 cgtagagagc tccgtcggcg caggtatgaa cgatgcggac cggccactca gcagcggtcc
      721 gggcccgtcg cggactgacg aggagatcca gatcgtcttc gatcgataca aggcggcgct
      781 gtaccgaatc tacaatcgcg agctacggac cgacccaacg ctccggggca agatggtgct
      841 gcgcatgacg atcgagccca atggagaggt atccgcgtgc accattgcgt caagcgattt
      901 ggcgtcggac gtgctcaaga aagaggttgt cgaacgggtg ctgcgattta actttggccc
      961 gaaagacaac gtgtccagaa tcaccattct gtaccccatc gatttcttgc cggcctcgtg
     1021 aagcaagagc aggcacaggg atcaagggag aaggatcaag ggagaaggat caagggagaa
     1081 ggatcaaggg agaaggatca agggagaagg atcaagggag aaggatcaag ggagaaggaa
     1141 catgcgtgca tgaaaatctc gaggagtcgc ggcagttgtg atgagcgtac ccatgacgtc
     1201 ctgtagcgca cgattcgttt ttcatcacat ccatatggcc gtcgctgtgg ctcaataggc
     1261 ggctcgcgcc agcggcgaat tcccgttagt cggtatcttg ttgcttgatc gtcagatcga
     1321 gcgacatgga ttcgtctgtg cgtcgcgcat ggcatcgcta agtgcgatca catgatgtgc
     1381 ggtcatttgc ctgataaccg gcatggcaaa taacgctcgg aaagctaaac ctaagtgatg
     1441 atctggatga acaatgatgc gcaataggct gagcccgatt cagaaaatgc cactcgtggt
     1501 ccgaggccgg tgatggcaca acgctttcct gactgggtac cgtttctgcg tgccgacatt
     1561 ttccacgccg cggcgtccgc gttccgcttt ggcaaagcgg tcgcatccgg cgagtaacgc
     1621 aaagaaatgt tccggcgaga tcaaatcaga gcgagggcag gcggccgctg gatcggtatc
     1681 cgggtgctgg ccttcgtact cttgctgggc gcggccttcg ccgcacatgc gggaactgat
     1741 ttttgcagtg gctacccgct cgtgaatggt taccacgtaa tcgacggcaa tgatcccacg
     1801 cttactcctc ttactcttcc ttcctcgatc gggatcgacg cgaattgtgt tttccagaac
     1861 tttccgatat ctgccaaatg gccgcagggt ctgacttcca ctctcaattt taaattcgat
     1921 gggtacttgg cgatttttaa caatgtttac tacaccggca atatggcgtg tgccactacg
     1981 accaccaaga tctggtttgt taaccccggc gcgatatatg accccaataa cagctgtcag
     2041 gatcttttca tacccgtaga gaaaattgat aagcagagcc ccgcgcctta cgccaccatc
     2101 ggagtgccct tcacttacaa gctcacgatc ccggtcatgt atgacccgtc caccggcacc
     2161 gtcatcggcg ccagcgggtc ggccaacgac ttgcactcca tcactgtcac ggacgatctc
     2221 aacgcgacgg gcgtggatct cacctattta ggctacgtcg cctactggaa gggcagcggc
     2281 acttcagtgc cgcttacatt ctccaacgcg ggcggcgtgc tcagctttag caactttccc
     2341 atcatcccgg ccggacagca gatcgtgatc gagatcacgg tcgtcctcaa tgacacgccg
     2401 acgaacgtgg ccgggaaggt gttcacgaac accgccgcgt ggtctttcgg gagactcatc
     2461 gatggcacgt tttacagccc cctcccgggc cagaatggga tctcgcagcc gatgaccatc
     2521 gctgagccca atctcgtggt taacaagagg agcaccgcga cggccctgaa cctgggggtc
     2581 atggcgccgt tcactatcga tgtccagaac accggcggca gc
//
LOCUS       DATIFR010000190         9074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2807345, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000190 DATIFR010000000
VERSION     DATIFR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2807345"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..544)
                     /locus_tag="VLD60_10905"
     CDS             complement(194..544)
                     /locus_tag="VLD60_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSB45514.1"
                     /translation="MGLADNKCIPCRGGVPPLPSDRIQALLKELGRGWSLNAQGHLER
                     LYTFKDFAQALAYVNKVSAVAEAEGHHPDLYLAWGKCKVEVWTHKINGLTESDFYLAA
                     KADREFEPFRAAAS"
     gene            complement(618..1616)
                     /locus_tag="VLD60_10910"
     CDS             complement(618..1616)
                     /locus_tag="VLD60_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HSB45515.1"
                     /translation="MAEAKAKKVRSTTAVRRRRKPAGSSSGLAATELQAAAPPGVVAE
                     LHTAIDQDGGKVLAMYREPYGGRWLVLAALPIDLVEPTPYQRNLSDTHVRKLEAVIGK
                     IGRFLDPIIAVRLSKPDHFAKYWTPNGNHRLSAMKTLGAKSIVAIVVPEAAAAYQILA
                     LNTEKAHNLREKALEVVRMYKELALLDDATEDTYALEFEEPAFITLGLCYEERPRFSG
                     GAYHPVLKRVEEFLKKPMRAAIEVRRERARTVLALDDEIVKQVEALKAKGLTSPYLKS
                     FVVARVNPIRFRPKDAPPLAFEDALDRMTKAAAKFNPDKIKMDDLAKSGGISDDTE"
     gene            1704..3068
                     /locus_tag="VLD60_10915"
     CDS             1704..3068
                     /locus_tag="VLD60_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB45516.1"
                     /translation="MNLASEPSQTSDRPYQTIGWFTASCLLVSNIIGGGIFTTTGIMA
                     RDLGDPLLILLLWGVGGLFAIGGAMVYGELGSRLPHAGGDYVYLREAYGPLVAFLSGW
                     TSFTIGFGAAVAASAISFSSYALRVIPFDDERGWWAKSLSLGLLWLVTLVHCTGLGAG
                     GRLQRLITSTKVLAIGGFILGGLLAGAGHWDVLSARPAQAEPPIGAVAIALVIVTYCY
                     LGWNVAGFIATEIADPQRNLPRVMIGGTAFVASIYVLLNVVYLSALSISALAQEPIIP
                     VAEKAAAAIWGPSSGRAVAVLLCLSIAGAVSAMTWAGPRVYWAMAEDGAISRWLAKRH
                     YQTGVPVRAILFQTCWASVLILSGTFEQLLVYSGLVLALFMALTISAIFRLRSANLDH
                     AHHYRAPFYPFLPAALVVGAFTLVIYSVIERPSESLYGAATVLSGIPLYLFWKKMKGM
                     GSST"
     gene            complement(3801..4619)
                     /locus_tag="VLD60_10920"
     CDS             complement(3801..4619)
                     /locus_tag="VLD60_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HSB45517.1"
                     /translation="MKRYRVKPDSKLHLDTFDPDDTGEYKKTDQEKEKAKTITAKLIA
                     KLDGLQERLYANADRSMLIVLQGMDTSGKDGTIKHVMSGVNPQGCTVATFKTPSNSEL
                     AHDFLWRVHHEVPAKGHIGIFNRSHYEDVLITRVHKMISDKIAQQRLNQITEFEELLA
                     ENGTTILKFFLHISKDEQKTRLEERIQDPEKRWKWNSGDLEERKLWDDYMDAFEAVMS
                     ATSTEHAPWYIVPANRKWYRNLVVADRIVDALESMKLETPPAPEGVDFDKLKIV"
     gene            5098..5718
                     /gene="aqpZ"
                     /locus_tag="VLD60_10925"
     CDS             5098..5718
                     /gene="aqpZ"
                     /locus_tag="VLD60_10925"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HSB45518.1"
                     /translation="MPPRFHNLESGCSGVAFAFGLTVLTMAYAIGHISGCHLNPAVSV
                     GLVVGKRFSANDLPGYIVAQVIGAILGAGVLYLIASGKPGFDLGGFAANGYAEHSPGL
                     YSLAAAFIAEVVLTFMFLFIILGATDSRAPQGFAPIAIGLGLTLIHLIGIPVTNLSVN
                     PACSTGPALFVGSWALAQLWLFWIAPIIGAALAGVAYPIVAGNQKR"
     gene            6042..6530
                     /locus_tag="VLD60_10930"
     CDS             6042..6530
                     /locus_tag="VLD60_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HSB45519.1"
                     /translation="MIKEVAGDLLLSKAHAIGHGIAPNDDFKQGLALSLREQWPGMYK
                     DFRHYCQTYNPKPGTLWSWKGPGSPIIINLFTQEPAEDHRGKPGKATIPYLNHVLQAL
                     KKELKVQQVTSLALPRLATGVGGLSWDEVKPLVEQALGDAGIPVYVYSTFKKGLAAAE
                     SV"
     gene            complement(6567..7655)
                     /locus_tag="VLD60_10935"
     CDS             complement(6567..7655)
                     /locus_tag="VLD60_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB45520.1"
                     /translation="MKRIRLTRVIVLAVCVSAGLTAAASVQANEPVAPKQESTPTPVP
                     PSAKPRTQDGEIKPAPKSSAAPSPAESTGKEPPQAKPEPGPGAAPPPSKPRMPEGEAK
                     PPAKSQHPANGAAASKPATVQFPAEITGKDGVPMVLVPVGEFTMGSDKGDDDEQPVHR
                     AFLDSFYIDKFEVTNGRFAKFVEAIQSEPPWGFADKETPVLHPDQPVRWVNWMDATGY
                     CLWAGKRLPTEAEWEKAARGTDGRNYPWGNDPPTPAHAVFGLKEGADTVSSIGNRDKG
                     KSPYGVHDMAGNLYEWTADWYDEEFYLKNPAINPRGPAEGTAKVQRGGSYTNTPYRLR
                     STFRTKGDPTEHDPNVGFRCAQDAPKQP"
     gene            complement(7910..8473)
                     /locus_tag="VLD60_10940"
     CDS             complement(7910..8473)
                     /locus_tag="VLD60_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HSB45521.1"
                     /translation="MNDDLIKHLGPLASLAGVWEGDKGDDVAPSDDRGTERNKFRERM
                     TFEPFGPTMNHEQALYGLRYSTVAWRIGEDAPFHEETGYWLWDSAAKQVLRCFIVPRG
                     VTVLAGGTAEPDAKTFELSAEAGSDTYGICSNKFLDREFKTVRYELTITVHGHDSFSY
                     KEDTQLKIKGQKDIFHHRDANTVKRVR"
     gene            complement(8802..>9074)
                     /gene="ssrA"
                     /locus_tag="VLD60_10945"
     tmRNA           complement(8802..>9074)
                     /gene="ssrA"
                     /locus_tag="VLD60_10945"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1782 a         2625 c         2605 g         2062 t
ORIGIN      
        1 gcacgccgat cttgtgcgca aatcggacgt tgagatggtg gcagtcgacg gcgatgcctt
       61 tggattttag gagggccgag agcgtcccga gctggatcga ggggtacttc gaatagctga
      121 acggcatgtt gacgagcgcg accctggcct tgtcgctcat ctggtgcctc gtaacgtcat
      181 cgcgatgatc cggttaactc gccgcggccc ggaacggctc gaattcccgg tcggccttcg
      241 ccgcgagata gaaatcgctt tcggtcaacc cattgatctt gtgcgtccaa acctcgacct
      301 tgcacttgcc ccaagccaga tacagatcgg gatgatgacc ttcggcttcg gccacagcgc
      361 tcaccttgtt cacgtatgcg agcgcctgcg caaaatcctt aaaggtgtaa agccgctcga
      421 gatgtccttg agcgttgagc gaccagccgc ggccaagttc tttcagcaga gcctggatac
      481 gatcgctcgg aaggggcggc acgccaccgc ggcatggaat gcacttgttg tcggccagac
      541 ccatggcagc ctccttcggt gacaggatca caatattcta atgggccggt gagcggagaa
      601 acaaggcggg gaggcggcta ctcggtgtca tcggaaatgc cgccggattt cgccaaatca
      661 tccattttga tcttgtccgg attgaatttg gctgccgcct tggtcatgcg gtcaagcgca
      721 tcctcaaacg cgaggggagg ggcgtctttc ggacggaagc ggatcgggtt cacgcgggcg
      781 accacgaaac ttttcaagta ggggctcgtc aggcctttgg ccttcaaggc ctcgacctgc
      841 ttcacaatct catcatcgag tgcaagcacg gtcctcgccc gttccctccg cacctcgatt
      901 gccgcacgca tgggcttctt gagaaattct tctacccgct tgagcacggg atgataggcc
      961 ccgccgctga accgtggccg ctcctcgtag cagaggccca acgtaatgaa cgccggttct
     1021 tcaaattcca aggcgtaggt gtcctcggtc gcatcgtcga ggagcgcgag ctctttgtac
     1081 attcggacaa cttcgagggc tttctcacga aggttgtggg ctttctccgt gttcagagca
     1141 aggatctgat aggctgcggc tgcctccggc accacaatcg ccacaatgct tttcgcgccg
     1201 agcgtcttca tggctgacag acggtggttg ccattcggtg tccagtactt cgcgaaatga
     1261 tccggtttcg aaagccgtac cgcgataatc ggatcgagga aacgcccgat cttgccaatc
     1321 accgcttcca gcttgcgcac atgagtatcg gaaagattcc gctgataggg cgtcggttcg
     1381 actaagtcga tcggcagcgc tgccagcacc agccagcggc cgccataggg ctcccgatac
     1441 atggcgagaa ccttgccgcc gtcctggtcg atcgctgtgt gcaactccgc caccacaccg
     1501 ggcggtgcag cggcttgtag ctcggttgcc gcaaggccgc ttgaggatcc ggccggtttc
     1561 ctgcggcgtc gcacggcggt cgtactgcga accttttttg ccttcgcctc tgccatggca
     1621 cgtatggtat gcgagcgcga tcagaaaggg aaggtgccgg ctgctcgcaa ttgtcgccac
     1681 cggcagcata cactggcgca caaatgaatc tcgcatcgga accgagtcag acttcagaca
     1741 gaccttacca gaccatcggc tggttcaccg ccagctgtct cctcgtcagt aacatcatcg
     1801 ggggcgggat ctttactacc acgggaataa tggctcgaga tctcggggat cctctgttga
     1861 ttcttctcct gtggggcgtc ggaggattgt tcgccatcgg cggggccatg gtttatggcg
     1921 aacttggctc tcggctgcct catgcggggg gcgactacgt gtatttacgc gaagcgtacg
     1981 gacccttggt cgcgtttctg agtggatgga cctcctttac gatcggcttc ggcgcggcag
     2041 tcgccgcctc ggctatcagc ttttcctcct atgccttgcg tgtcattccg ttcgatgacg
     2101 aacggggctg gtgggcgaaa agcctatcgc tcgggctgct ctggctcgtc acactggtcc
     2161 attgcacagg gctcggagcg ggtggccggc tgcagcgcct tatcacgagc accaaggtgc
     2221 tcgccatcgg cggattcatt ctcggcggct tgctggcggg cgcgggacac tgggatgtgc
     2281 tttccgccag gcccgcacag gcggaaccac ccatcggtgc agtggcgatc gccttggtga
     2341 tcgtgaccta ttgctacctt ggttggaacg tcgccggctt catcgccact gaaattgccg
     2401 atccgcaacg aaacttgcca agggtcatga tcggggggac ggcatttgtg gcctcgattt
     2461 atgtgctgct gaatgtcgtg tatctctcag ccctctcgat cagcgcgctc gcccaagaac
     2521 cgatcatccc ggtggctgaa aaggcagcgg ccgccatatg gggaccgtcg agcggccgcg
     2581 ctgtcgctgt gctcctctgt ctctcgatcg cgggagccgt cagtgcgatg acctgggcgg
     2641 gacctcgcgt gtactgggcc atggcagagg atggagccat cagtcggtgg ctcgccaaac
     2701 gccattatca gaccggcgta ccggtccgcg cgattctctt tcagacatgc tgggcctcgg
     2761 tgctcattct gagcggaacg ttcgaacagt tgctcgtcta cagtggattg gtcctcgccc
     2821 ttttcatggc cttgaccatt tcggcgattt tccgacttcg cagcgcaaac ctcgaccacg
     2881 cccatcacta ccgcgctccg ttctatccct tcttgcccgc ggctcttgtc gtcggagcct
     2941 ttacactggt catctacagt gtgatcgaac gtccttcgga gtcgctctat ggggctgcga
     3001 ccgtgctcag cgggattcca ctctacctct tctggaagaa aatgaaggga atgggttcgt
     3061 caacatagct tccggcaaaa ccgcagcggg cgatagggta aggtgtactc agcgtggtat
     3121 gtcgaatcct tgagtgatgc gagaacgaaa tggacaactg cttttacgga ctcaacacgg
     3181 cacgggcgac cttgcactgt ggtgggaggt cgcccgtgat tggcgcttag gacgccagta
     3241 ttgatgatgg attgtcgcgg cccgaaagac ccgcgccatg ccaggttaag cgagtttcca
     3301 gtcgggggag acgtcgtcga tcccaggcgc ttgtgttcct cgtgcccgca tcaaattctc
     3361 ccgcggatca ggctgatatc cggttggtct ttggtgccat gacggcacag ccagcggaga
     3421 accggctagc acgaccgacg agaaacccgc cctcgcatcg gcgggcctga cgaaccaggt
     3481 ggagtcgcat cgtccatatt tcgaccctcc tctcttggtg aagtcaccga gagacctgga
     3541 tgacagccgc gcggtctgcc cgatctctcg aaccgcctca aataaaatgg gccgcaagtt
     3601 cctcctacgc ccattagaaa ttcatgtcaa tggggcggga aaagttttgt tccaacgcat
     3661 aacacatgcc agccgacacc tgatggtcca gcagcaaata ctatcttcaa ccaatcccga
     3721 tcggccacag ggccgatcca ggataggccc gataaatcct gtgcacatcg aggaaaccgg
     3781 cgacgtcgaa tgcttggtcg ctacacaatt ttgagcttgt caaagtcaac gccttccggc
     3841 gccgggggtg tttcgagttt catggattcg agcgcgtcga cgatccgatc ggccacgacg
     3901 agattccgat accatttccg attggccggc acgatatacc agggggcgtg ctcggtactg
     3961 gttgccgaca tcaccgcttc gaacgcgtcc atatagtcgt cccacaactt gcgctcttcg
     4021 aggtcgccgg aattccattt ccatcgcttc tcgggatcct gaatccgttc ctctaatcgt
     4081 gttttctgct cgtctttgga aatgtggaga aagaacttga gaatggttgt cccgttctca
     4141 gccaataatt cttcaaattc cgtgatctgg tttaatcgtt gctgcgcgat tttgtctgaa
     4201 atcatcttat ggacccgcgt gatgagcaca tcttcgtaat gagaccggtt gaagatcccg
     4261 atgtgtcctt tggccggcac ttcgtgatgg acgcgccaga gaaagtcatg ggcgagctcg
     4321 ctgttggaag gggtcttgaa tgtggccacc gtgcagccct ggggattcac accggacatg
     4381 acatgcttga tcgtgccatc ctttccgctc gtgtccatgc cctgaagtac gatgagcatc
     4441 gatctgtccg cgttggcata gagccgttct tgaagtccgt caagtttcgc gatgagtttt
     4501 gctgtaattg tcttggcctt ttccttctcc tggtccgttt ttttgtactc gcccgtgtca
     4561 tcggggtcaa acgtgtcgag gtgcagtttc gagtccggct tgacacgata ccgtttcatg
     4621 gtcgcttcct ccctttctgt tcaggctcgt gacagagacg atattctact ccttccccct
     4681 catcttggca ggcgagcgcg actcgcaaca aatgcggtcc tttgtaccga agaacggcct
     4741 cattagccta taacggaccg gccttggtcg cgcaagcagg agaagcgcgg ttgcgcgagg
     4801 tcgtcatgaa gggatgtttt acccgttttc gtcgcgcgac ccagacaccg tttaatatcg
     4861 tattggaggc aagacgccag aaggcgcgac acggtccgcc tttctcgttc cagtttatcc
     4921 ccgacaaggc tagcaacgaa tcaaacacct gccgcatgtg acatatcctc tgaatcggag
     4981 tatggtcaag cagcggtgca caccataaag gaggacttat accggcaatg aatcgcttac
     5041 tcgcggagac catcggcacg ttctggctgg tcttcggagg atgcggcagc gccgtacttg
     5101 ccgccgcgtt tccacaactt ggaatcgggt tgctcggggg ttgcattcgc attcggtctc
     5161 accgttctga ccatggccta tgccattgga cacatttcag gttgtcatct caatcccgcg
     5221 gtctcggtgg ggctcgtggt aggcaaacgc ttctccgcca acgatctgcc cggctacatt
     5281 gtcgcgcaag tgatcggagc cattctgggt gcaggagttc tctatctgat cgccagcggg
     5341 aaacccggat tcgacctcgg cgggttcgcc gccaacggct atgcagagca ctcgcccggt
     5401 ttgtactcgt tggcggcggc attcatcgcc gaggtggtgc tcaccttcat gtttctcttc
     5461 atcattcttg gggcgactga cagccgagcc ccgcaaggtt ttgcgcccat cgctatcggc
     5521 ctcggcctga cgttgattca cttgatcggc attcccgtca ccaatctttc tgtgaatccg
     5581 gcatgcagta caggtcctgc cttgtttgtg ggcagctggg cactggctca actctggcta
     5641 ttctggattg ccccgatcat cggggccgca ctggcaggag tggcctaccc gatcgtcgcc
     5701 ggcaaccaga agcgctaacg tcagctcgac gaccaggccg aacgagctat cgcgtggagc
     5761 gtctagattg gaatcgacgg aattcctttg cccctcggga ttctcgacgt ccgctccgga
     5821 tccaacgcgc gaaagttgag accgccaacc gcaccgccgc agcgaacccc atcatggaag
     5881 cgagaccgcg acatgatgac cgaccatagc agggagtgat gtgccgaggg atcgagctat
     5941 ttcattccag cgctgttgac ccagtgcgca gagagcctta tgttgtgcct tgtaatgtag
     6001 cggcaatcat tcagataact tcagaaagga aacagccttt tatgattaaa gaagtcgcgg
     6061 gagatcttct gctctcaaag gcccacgcca tcggccacgg tattgcgccc aacgatgatt
     6121 tcaaacaggg gttggctctc tcgcttcggg agcagtggcc cggcatgtat aaggatttcc
     6181 ggcattactg ccaaacgtat aatccgaagc ccggtacgct ttggagctgg aaaggcccgg
     6241 ggtcgccgat catcattaat ctcttcacgc aagagccggc ggaggatcac cgggggaaac
     6301 cgggtaaagc gacgatcccc tatctcaacc atgtcctcca agcattgaag aaagaactca
     6361 aggtgcaaca ggtgacgagc ctggctctgc cgcggctggc cacaggggtc ggcggtctct
     6421 catgggatga ggtcaagccg ctggtcgaac aagcgctggg ggatgcaggc attccggtct
     6481 atgtatactc gacgtttaag aaaggactcg cggctgccga gagcgtgtga gcgacggtgc
     6541 gaaactcagg gaccatcagg aatcactcag ggttgtttgg gggcatcctg ggcacagcgg
     6601 aagccgacgt ttggatcgtg ttcggtggga tcacctttgg tgcggaacgt tgagcgcaac
     6661 cggtaggggg tgttggtgta agatccgccc cgttgcactt tcgcggtgcc ctcggcaggg
     6721 ccgcgcgggt tgatggccgg attctttagg taaaactcct cgtcatacca atcggccgtc
     6781 cattcgtaca aattccccgc catgtcatgc acgccatagg ggctcttgcc tttgtcgcga
     6841 ttgccaatcg atgagacggt atcggctcct tccttcaagc cgaacactgc atgcgccggc
     6901 gtcggagggt catttcccca aggataattg cgcccatcgg ttccgcgagc agctttctcc
     6961 cactccgctt ccgtcggcaa tcgcttcccg gcccacaggc aatagcccgt cgcgtccatc
     7021 caattcaccc agcggaccgg ttgatccgga tgcagcaccg gcgtttcttt atcagcaaac
     7081 ccccagggcg gctcgctctg aatggcctcg acgaatttcg caaaccggcc gttggtcacc
     7141 tcgaacttgt cgatatagaa actgtcgaga aatgcccgat ggaccggctg ttcatcgtcg
     7201 tcacccttgt cgctgcccat cgtgaattca ccaaccggga caagcaccat cggaaccccg
     7261 tctttcccag tgatctcagc aggaaactgg actgtagctg gttttgacgc ggcggctcca
     7321 ttagccggat gctgggactt ggcaggaggt ttcgcctcgc cttctggcat gcgaggtttt
     7381 gacggcggag gtgccgcccc aggaccaggc tccggcttcg cttgcggcgg ctcctttccc
     7441 gtggattcag ccggcgacgg agccgcggaa ctctttggcg ccggcttgat ctcgccgtcc
     7501 tgtgtgcgtg gcttcgccga cgggggaacc ggtgtcgggg tcgattcctg tttgggggca
     7561 acaggctcat tcgcctgtac cgatgcggcg gcggtcaaac cagccgacac acataccgca
     7621 aggacaatca ctcgagtcag tctgatcctc ttcattctga gaaagcaggc tccatcgatc
     7681 gatgacgacg cagttgaaaa gtgcggcttc agcaattcag ggtaccgaaa aagacgagaa
     7741 caaatctaga gcagggggct ggttttcccc gaccatcaat cattttaacc attattcgta
     7801 ggctggagca tacagtcagg gccatctttt cagaaatggg aggtcacgag tggcaggatg
     7861 agacggcttc cgactcaagc tctgtcacct cgaaccgcaa ccgcttcttt tatcgtactc
     7921 gtttcacggt attggcgtcc ctgtggtgga agatgtcctt ctgtcctttg attttcagtt
     7981 gagtgtcttc cttgtagctg aagctgtcat gaccatgcac ggtgatcgtg agttcatacc
     8041 ggacggtctt gaattcccga tccaggaact tgtttgaaca gatgccatag gtgtccgacc
     8101 ctgcctccgc cgagagctcg aacgtcttcg catcgggctc ggccgttcct ccagccagca
     8161 cggtcacgcc ccgaggcacg atgaaacaac ggagcacctg tttggcggcg gagtcccaga
     8221 gccagtaccc ggtttcttcg tgaaacggag catcttctcc gatgcgccag gcaacggtcg
     8281 agtatctgag accgtagagc gcctgctcgt gattcatcgt cgggccaaat ggctcgaacg
     8341 tcatccgctc gcggaacttg ttgcgctctg tgcccctgtc gtctgacgga gccacgtcgt
     8401 cccctttgtc tccctcccac acaccggcaa gtgatgccaa gggaccgaga tgcttgatga
     8461 gatcgtcgtt catggtgctc cctttatgtt gttccgaata ttcgaaagta atctgactgt
     8521 acggggggaa ggaccgaggg cacgttctag caaactctcg tgagaagaat caatttacgg
     8581 tcatgggcgg ggagacgttg aagagcgcgt ggcaggcgca agtagtataa gacaaagaac
     8641 cggatggttt tatccggcgc cgtttatcgc cataaaacga taaaaaggcg ccggcacgca
     8701 cgaaggtctg tagcagaaca tgaaaacact tgaggcatct atccgcgggc gtcaatctcg
     8761 atagatcgaa aaagagcccg cgggtcaacc gaaggttggt atggtggagg cgaggggagt
     8821 tgaacccctg tccgaagatc gtcagtgcac tgtgtctaca tgtgtagccg atcgtttaga
     8881 tttcgcagcc acccacgccc atcggcaggc ttagaatagc cgctagccca gagcattctc
     8941 gtcctcagcc gctgagcacc agccttggac cagcccgctg catcgcgctc caaccacccc
     9001 cgcgggcatc aggtggagga acgtcgcagt tatttaagct gcgagtgcca gttcttgatt
     9061 ggcagttgca tttt
//
LOCUS       DATIFR010000191         6639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_318808, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000191 DATIFR010000000
VERSION     DATIFR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318808"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VLD60_10950"
     CDS             <1..814
                     /locus_tag="VLD60_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSB45522.1"
                     /translation="RWFRHRSRFYQTTGRYRTPQRHRYGWLGSLLVTGYLGLIVGAAF
                     ALPAYLLVTWSLSPEAQATVDSRFYGFLWNSGFLAACAATGGVLIGLPLAYLASRRPT
                     WLNLGCLQAAYAGYVLPGPVAALAVLVLCLNLTPFIYGSVLVLIIAYVIHFLPAGLQS
                     LEPALQQITPNLEEVARTLGLGVRQTWQRITLPLVRNGFVVAWVLMFLQTMKELPATL
                     LLRPVGFDTLAIRVWMEASEEYYQLAAPSALLIVLLSLPTLVLLVSKDWRAA"
     gene            833..1987
                     /locus_tag="VLD60_10955"
     CDS             833..1987
                     /locus_tag="VLD60_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB45523.1"
                     /translation="MKPDHSIAHGPFSESTLQADLYTPASSVLELRVVSCAYETGRPA
                     VRDISIAAREGEILCLLGPSGCGKTTILRAIAGFEPVRSGQIFLSGRLVSSPDQMTPT
                     EERHVGMVFQEYALFPHLRVQDNIAFGLRHLSRSERKARVQDMLRLIGLEGFDRRYPH
                     ELSGGQQQRVALARALVQHPVVLLLDEPFSNLDPDMASRMRQDLHDLLRRTKTTTILV
                     THDHDEAFALADRIAVLNQGRLEQFDTPETIYHMPETPFVADFVGQADFIPGTVSNGM
                     VYTELGEFPNTLDCEEGATVVVMIRPDDIHLVPTKGATSHVVSRQFRGSENLYAVSLP
                     SGQVVHSSQGSTSVYHEGTPVELRVLASHTVLFKQSDQSPSAGSPLKPAH"
     gene            2085..2516
                     /gene="exbB"
                     /locus_tag="VLD60_10960"
     CDS             2085..2516
                     /gene="exbB"
                     /locus_tag="VLD60_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HSB45524.1"
                     /translation="MDALKNAVDYGIIGLLLALSVWSVAVAVERWLYYRRIDLGQFSS
                     LQSLEIALTKRLVIIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNMLRRKVAEIIAQYKVQDGSRS"
     gene            2500..2874
                     /locus_tag="VLD60_10965"
     CDS             2500..2874
                     /locus_tag="VLD60_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB45525.1"
                     /translation="MDREVNQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EVGDRKDVPVVITLNAEGLLFLNDTPVPENGLNDALTAFPRESAVVVRADKTTLLERF
                     VSLVDDVRGLGFQQVSLEVVRL"
     gene            2871..3917
                     /locus_tag="VLD60_10970"
     CDS             2871..3917
                     /locus_tag="VLD60_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSB45526.1"
                     /translation="MTTTNRTAASTFTGAHTAGWIASLILHGTLAFGAFVFTQQIRLA
                     PQLTPFQWNVAMVTSEQRSMPTPSPVTSPDHAQPTTKPTAATPQVQAQAVQPVSPTRP
                     EPAKVGPVQPLTPIASTPLSQTQPERQPEVAKPAPPVQAIVAAPAPVESVAQEPTAVR
                     PIDSPSQPAPTEPAPLSANREPAKHIETSPAIYETFAPPPPDSTTAPVPEQPVTATPS
                     QPYSAQSAPSEATSTTAAPQIAALAPTPSAAPPRQDYGWLSDTILRRVEELKRYPPEA
                     RLDRAEGKVVLKAVIRADGSVDDVEIYQSSGYRSLDQAAVELMKRAAPIQLPRPLGKA
                     KMTVKIPMSYRLEP"
     gene            complement(3932..4831)
                     /locus_tag="VLD60_10975"
     CDS             complement(3932..4831)
                     /locus_tag="VLD60_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSB45527.1"
                     /translation="MGTLVSITAVAVNHDAAKQVINAGFQEIKRLEQLLSTWISDSEL
                     SRVNDSAGIKPVPVSPETLTVVLRSLQVAEVTNGAFNIVIGPAIGAWNISAQPRVPTT
                     AELDALRPLLDLQAVRADVSKRTLYLEKMGMRIDVGGIGKGYAADQAVVAMQKAGAVA
                     GVVALSGDIKAFGRLPGERPFPVGIQHPRKEGAVLVWIDLQDEAISTAGDYERYFEKD
                     SVRYHHILDPQTLQPARRCQSVTIVAREGIWADGLDTGIFVMGPERGMELVERLADVE
                     AIIVDAEGRMSVSSGLKNRIRFP"
     gene            complement(4970..5548)
                     /locus_tag="VLD60_10980"
     CDS             complement(4970..5548)
                     /locus_tag="VLD60_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45528.1"
                     /translation="MTLLITLSGCVTSLPAAVDSSDETGLLAVGRVSTVITGETRRIY
                     GPSLRFFELVNRQTHERFRVDVQSDDQQFVLSLPPGEYELDRVQINEGPFMSMAQLAA
                     SFALQEGVITYLGTWRFGVDSPRYGRMVVLSMIVDEQDRDQTEEVIRQKYPAHEVQSI
                     VTVLPNPPESETRLYEVMSYPRVPRYFQRHQW"
     gene            6046..>6639
                     /locus_tag="VLD60_10985"
     CDS             6046..>6639
                     /locus_tag="VLD60_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45529.1"
                     /translation="MRIRSILGALVLSGLSVTPIWAENMTPEEIKKLVDEAVEKRLQE
                     HERREGATERREPAPPSGQYPTGVGPMTDVKVERPGEEKVPLSFGATGSGRLVYAKPF
                     VAAPKAIVGGYMDIQYRAQSKASIENGYGGNTNGFDQQRFVPFIYADITEHVKFASEL
                     EIEHGIRETSDNEISLEFAHIDYLVNEPFNVRAGILLL"
BASE COUNT         1450 a         2050 c         1783 g         1356 t
ORIGIN      
        1 acgatggttc cgccatcgca gccggttcta tcaaacgacc ggacggtacc ggactccaca
       61 gcgtcatcgt tatggatggc ttggaagctt gctcgtcacc ggctacctgg gcttgatcgt
      121 cggcgcggcg ttcgcgctgc cagcctacct cttggtcacg tggagccttt cccccgaagc
      181 tcaggcaacc gtggatagcc gcttttacgg cttcctgtgg aacagcggtt ttctggctgc
      241 ctgcgctgca accggagggg tgctgatcgg attgccgttg gcatacctgg ccagccgccg
      301 cccaacctgg ttaaacctgg gctgcctgca agccgcctac gcgggctatg tgcttcccgg
      361 accggtagcg gcactggcgg tgctcgtcct ctgtttgaat ctgaccccat ttatctacgg
      421 ttcggtcttg gtcctcatta tcgcgtacgt gattcatttt ctcccggccg gattgcagtc
      481 cctcgagccg gcgctccagc agattactcc gaacttggaa gaggtggccc gtacgctcgg
      541 tctcggcgtt cgccaaacct ggcagcggat cacgctgccg cttgtgcgga acggattcgt
      601 cgtggcctgg gtgctgatgt ttctccaaac gatgaaagaa ttgcccgcga cgctcttact
      661 gcgcccggtg gggttcgaca cgttagcaat tcgtgtctgg atggaagcca gcgaagagta
      721 ctatcagttg gcagccccgt ctgcactgct gatcgttctc ctcagcctcc ctacgttggt
      781 tttgttggtg tcgaaggact ggcgggcggc gtaaccgaag gataattagt acgtgaaacc
      841 ggatcactcc atagcccacg gccctttcag cgagtcgacg ctgcaagccg atctgtatac
      901 gccggcatcg tccgtattgg agcttcgcgt cgtctcgtgt gcctacgaga ccggccgccc
      961 ggcggtgcga gacatctcga tcgcagcccg tgaaggagag atcttgtgtt tgttgggccc
     1021 gtcgggatgc gggaaaacca caatccttcg agcgatagcc gggtttgaac cggtacgttc
     1081 ggggcagatc tttttgtcag gacgacttgt ttcatcgccc gaccagatga cgccgacgga
     1141 agagcggcac gtcggcatgg tctttcagga atatgccctg tttccacatt tgcgcgtgca
     1201 ggacaacatc gcgttcgggc tccgccacct cagccggagc gaacgcaagg cgcgcgtgca
     1261 ggacatgctc cggttgatcg ggcttgaagg attcgaccgc cgatacccgc acgagctgtc
     1321 cggcggacag cagcagcgcg tagcccttgc cagggcgctc gttcagcatc ctgtggtcct
     1381 gctgctcgac gagcctttca gcaacctgga ccccgatatg gctagccgca tgcgtcagga
     1441 cctccatgac ctgttacgcc gcacgaagac cacgaccatt ctcgtcaccc acgaccatga
     1501 tgaagccttc gccctggcgg atcgtatcgc cgtgctcaat caaggccggc tcgaacagtt
     1561 cgacacgccg gaaacgatct accacatgcc ggaaaccccc tttgtggccg atttcgtcgg
     1621 gcaggccgac tttatccctg gcacggtctc caacgggatg gtctacacgg aactcggcga
     1681 gtttcccaac acgctcgact gtgaggaggg cgccacggtt gtcgtaatga tcaggccgga
     1741 cgacattcat ctggtaccga ccaagggagc gacgtcgcat gtggtctccc gccaattcag
     1801 aggctccgaa aacttgtacg cggtgagcct gccctcgggc caggtcgtcc acagcagcca
     1861 gggctcgacg agcgtctacc acgaaggcac ccccgtggaa ctccgcgtcc tcgcgtctca
     1921 cacggtgctc ttcaagcaat ctgatcaatc gcccagcgcc gggtctcccc taaaacccgc
     1981 acattgacag ccgttttgcc cttctggcag catgggggct catacacacg catcccaacc
     2041 cctccaggcc ccgaacgtca cctttggagt tgaaggagcc gctgatggac gcgttgaaga
     2101 acgcagtcga ctacggaatt atcggtctgc tactggcctt gagtgtctgg tccgtcgctg
     2161 tggccgtgga acgctggctg tattaccgcc ggattgatct cggccagttt tcgtcgctcc
     2221 aaagcttgga aatcgccctc accaaacggc tggtcattat cgggaccgtc gccgccaatg
     2281 ctccctacat cggccttctc ggcactgtcc tcggaatcat gctcacgttc catacgatgg
     2341 gaacctcggg gacaatggcc gtcaatacca tcatgatcgg actcagcctg gccttgaaag
     2401 cgacagccgt tgggctgctc gtggccatcc cctgcgtcgt catgaacaac atgctgcgac
     2461 gaaaagttgc tgagatcatc gcccagtaca aggtacagga tggatcgaga agttaatcaa
     2521 attaacgtga tcccgctcgt cgacgtgatg ctggtcctgc tcgtcatcgt gctgacgacg
     2581 gccaccttta tcagcaccgg gcagatcccc gtcaatctcg cgaaagcgaa agaagtaggc
     2641 gaccggaaag acgtgccggt cgtcatcacg ttgaatgccg aaggcctcct gttcttgaac
     2701 gacacaccgg tgcccgagaa tggcctgaac gacgcgctca ccgcctttcc ccgggaatcc
     2761 gccgtcgtcg tccgtgcaga caagacgacg ctcctggaac gatttgtctc cctggtggat
     2821 gacgtgcgcg gcctcggttt tcagcaagtg agcctggagg tcgtccggct gtgacgacga
     2881 caaacagaac agcggcatcg acattcaccg gcgcacatac ggccggctgg atcgcttcgc
     2941 tcattctcca tgggacgctg gcatttgggg ccttcgtctt cacgcagcag atccgcttgg
     3001 ccccccaact gactcccttt cagtggaacg tcgcgatggt gacgtcagaa cagcgatcga
     3061 tgccgacgcc gtcgcccgtt acttctcccg atcatgcgca accgaccact aaaccaacag
     3121 ctgcgacacc ccaggtacag gcacaagcgg ttcagccagt cagcccaacc aggcctgagc
     3181 cggccaaggt cgggccagtt cagcccctca cgcctatcgc ctctacccct ctgtcacaaa
     3241 ctcagccgga acggcagcct gaggtcgcaa aaccagcgcc tcctgttcag gctatcgtag
     3301 cagctccggc accggtcgag tccgtcgccc aggagccaac agctgttcga ccgatcgact
     3361 ccccttcgca accggccccg accgagccag ctcctctttc agccaatcgg gaaccagcga
     3421 aacatatcga gacgagccct gcgatttatg aaacattcgc accgcctcca ccagactcga
     3481 ccacggcacc ggtaccggaa caacccgtga cagcaactcc cagccaacct tacagcgcac
     3541 agagcgcacc gagtgaagcc acatcaacaa cagcagcgcc gcagatcgct gcgcttgcgc
     3601 caactccatc agccgcaccg ccccgccagg actatggttg gttgtccgat acgatcttgc
     3661 gtcgtgtgga ggaactcaag cggtatccgc cggaagccag actcgaccga gcagagggga
     3721 aggtggtgct caaggcggtg attcgtgcgg atggcagcgt tgacgatgta gagatttatc
     3781 aaagctccgg ctatcgaagc ctcgatcaag ccgcggtcga actcatgaaa cgggctgccc
     3841 ccattcaatt gccgcgtccg cttgggaaag caaagatgac ggtgaagatt ccgatgagtt
     3901 atcggctcga gccgtagtgg cagccactcg ctcagggaaa ccgaatccga tttttcagac
     3961 cagacgagac cgacatccgg ccttcagcat ccacaattat ggcctcgaca tcggctagac
     4021 gctcaacgag ctccataccg cgctcaggcc ccatcacaaa aatccccgtg tcgagcccgt
     4081 ctgcccagat gccctcgcgg gccactatcg taacgctctg gcagcggcgc gccggctgga
     4141 gtgtctgggg atccagaatg tgatgatacc gcacactatc cttttcaaaa taacgttcgt
     4201 aatcgcccgc cgtcgagatt gcttcgtcct gtaagtcgat ccagaccaac accgcccctt
     4261 ctttcctcgg atgttgaatg ccgacgggaa agggacgctc ccccgggagc cgcccaaatg
     4321 ccttgatatc tcctgagagc gcaaccacgc cggccaccgc accggctttc tgcatggcca
     4381 cgaccgcttg atccgccgca tagcccttgc cgattcctcc cacgtcgatc cgcatgccca
     4441 ttttttccag gtagagcgtg cgcttcgata cgtccgcacg gactgcctgc agatccaaga
     4501 ggggcctcaa ggcatccaac tcagcggtag tcggaacgcg gggctgtgcg gagatattcc
     4561 acgcaccgat cgctggacca atgacgatgt tgaaagcccc attcgtcacc tcggctacct
     4621 gcaacgaccg aaggacaacg gtgagcgtct ccgggctaac cggcacgggc ttgatgcccg
     4681 cggaatcatt cacgcgagaa agctcgctgt cagaaatcca cgtacttaac agctgctcga
     4741 ggcgtttgat ttcttgaaac ccggcgttga tgacctgctt cgcagcatca tgattcaccg
     4801 ccaccgccgt gatggaaacc agcgtgccca tgtgcatctg agcacgtttg acaaccactg
     4861 gctctgagga atgtgacagg ctgctacagc ctatcatgac catcatgatt ggaactgcga
     4921 tgacgctagg aacaacactc gatcgatttc ttgcaatcat cgatcacttc taccactgat
     4981 gacgttggaa atatcgggga acacgggggt acgacatgac ttcatagagg cgtgtttcgc
     5041 tttccggagg gtttggcaga accgtgacaa tcgactgaac ttcgtgtgca ggatactttt
     5101 gcctgattac ttcctctgtc tgatcgcgat cctgctcatc cacaatcatt gagagaacca
     5161 ccatccgccc gtatcgcgga gaatccactc cgaatcgcca ggttcccagg taggtgatga
     5221 ctccctcctg caacgcaaac gaggccgcca gttgagccat cgacataaac ggcccttcgt
     5281 taatttggac acgatccagc tcatactctc ctggcggtaa cgacaacaca aactgctgat
     5341 catcagactg gacatccaca cgaaaccgct cgtgtgtttg tcgattcacc agctcgaaaa
     5401 atcgaagaga tggcccatag atgcgtctgg tctcgccagt aatcaccgta gagacgcgac
     5461 ccaccgcaag caaccccgtc tcatcgcttg aatcgactgc agcaggcaag ctggtgacac
     5521 acccgctcag ggttatgagc agcgtcacaa ctacccagtt ccagggtgac caaagccctg
     5581 ccgaaaccgc aaacatacgc cgattatagg gaatcagcgg tctcaatgca ccattcccga
     5641 gcgcagaaag ctgccagcta aagattactt gacgggagga ggcggaatag gatactaata
     5701 atcgttctca atttcattta acaaaaccta actccgatca tgcgtaccac acagccatca
     5761 tctgctcctc aatcgtacag cggcgagacc cgctgtgagt gcgggcaatt gatcgcaaag
     5821 gtgcgcggcc aaggattgga attgaagtgt aaacggtgca agcgaattgt ggtgatcccc
     5881 ttcactgcca tcgaaggatg gacggaagtt tcacctggtc acgcgtcatg aaaggaggtt
     5941 atcgctcagt tcccgtgcga acaatcacta tgaactctca tcacggccag agaccatcga
     6001 gtcccgagtc agaccatcac ccttattcaa tcatggaggt tttcaatgag gatccggtca
     6061 atcctcgggg ctctcgtact ctccggcctt tcggtcacac cgatctgggc agagaacatg
     6121 accccggaag aaatcaagaa gctcgttgat gaagcagtcg aaaaacgtct ccaagagcac
     6181 gaacgacgcg agggcgctac ggaacggcga gaaccggcgc caccgtctgg acaatacccc
     6241 acgggggtcg gtcccatgac ggacgtcaaa gtcgaacgtc cgggcgaaga gaaagtaccc
     6301 ctctcgttcg gcgccactgg ttcaggacga ttggtatatg ccaaaccgtt tgtcgcagcc
     6361 cctaaagcga ttgtcggcgg ttatatggac attcagtatc gtgcccagag taaagccagc
     6421 attgaaaatg gttatggagg gaacaccaac ggattcgacc agcaacgatt cgtcccgttt
     6481 atttacgctg acattacgga gcatgtgaag tttgcctctg aacttgagat tgagcacggg
     6541 attagagaga ccagtgacaa cgaaatcagc ttagaatttg ctcatataga ctatctcgtg
     6601 aatgaaccct tcaatgttcg cgccggcatt ttgctgctt
//
LOCUS       DATIFR010000192         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_511417, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000192 DATIFR010000000
VERSION     DATIFR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511417"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /gene="topA"
                     /locus_tag="VLD60_10990"
     CDS             <1..2278
                     /gene="topA"
                     /locus_tag="VLD60_10990"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HSB45530.1"
                     /translation="GRGYTVMASVGHIKDLPTSKLGVDLEHDFKPQYVTIKGKSKVLA
                     DIKKKAEEADKVYLAPDPDREGEAIAWHLAEELKGKSKKKSDGKVFRVLFNEITERAI
                     KQALQSPGQIDMKLVNAQQARRVLDRIVGYQGSQLLWNKVRRGLSMGRVQSVAVRLMC
                     EREQEREAFRAEEYWSITVLLAGTNPPPFEAKLHSINGEEASITTADEAQKVVAAIQG
                     KPFSVTSIERKEKKRNPVAPFITSRLQQEAARKLHFTPKKTMTLAQQLYEGVEIGAEG
                     PTGLITYMRTDSPRISQEATAEARQVIQDRFGAEYLPATPNVYKTQKAAQEAHEAIRP
                     TSASRDPESIRQYLEQDQYNLYKLIWNRFIASQMVPAILDVTRVESTPFETKGTYLFR
                     STGTIVKFPGHTIVYMEGVDKELFAQKQKGDQEVEDDAERQLPELHEGERLRLVEQAE
                     QTIPGITSKQHFTQPPPRYNEALLIKELEEKGIGRPSTYAAIISTIQDRKYVEKVEGR
                     FIPTETGRTVNDFLVKGFPQVVNVDFTSHLEEELDEVEEGNKPWVDAVRDFYDPFTRE
                     MERAKTIPGPKDTVEPPTDIPCEKCGRMMEIKWGRNGKFLACPGYKEDPPCKNTQNFE
                     KLADGTIKIVPKQELTTDQLCEKCNSPMVVKTGRFGKFIACSAYPQCKTTKPLALGVK
                     CPQPGCGGDLVQKRTRKGRSFFACSKYPACEYALWDRPINKACPTCSAPFLIEKVSKQ
                     AGRSVQCRNEDCGYREAG"
BASE COUNT          657 a          692 c          743 g          497 t
ORIGIN      
        1 tgggcgtggt tatacggtca tggcctcggt cggccatata aaggatttac ccaccagcaa
       61 gctcggcgtc gatctcgaac atgatttcaa gccgcaatat gtcaccatca aggggaaatc
      121 gaaggttctg gctgacatca aaaagaaagc cgaagaagcc gacaaggtct atctcgcgcc
      181 ggacccggat cgcgaaggag aggcgattgc ctggcatctt gctgaagaat tgaagggtaa
      241 gtccaaaaag aaaagcgatg ggaaagtgtt ccgcgtgttg tttaacgaga ttaccgagcg
      301 ggccatcaag caggcgttgc agtcgcctgg tcagatcgac atgaaattgg tgaatgccca
      361 gcaagctcgt cgcgtgctgg atcggatcgt tggctaccaa ggcagccaac tcttgtggaa
      421 taaggtccgg cgtggcctct ccatgggacg ggtgcaatcg gttgccgtgc gcttgatgtg
      481 cgagcgtgag caggaacgtg aagcgttccg tgccgaggag tattggtcga tcaccgtgct
      541 attggccggc acgaatcctc ctccgttcga ggcaaagctg catagcatca atggcgaaga
      601 agcctcgatc acgaccgccg acgaagcgca gaaggtggtc gctgccattc aagggaagcc
      661 tttttcggtc acgtcgatcg agcggaaaga aaagaagcgg aatccagtcg ctccgtttat
      721 taccagccgg ctgcagcagg aggccgcgag aaaactccac ttcactccca agaaaaccat
      781 gaccctcgcg cagcagcttt atgaaggtgt ggagatcggg gcggaaggcc ccaccgggct
      841 catcacctat atgagaacag actcgccgag aatttctcag gaagccacgg ctgaagccag
      901 gcaggtgata caagatcgat tcggagccga atatctccct gcaactccca atgtgtacaa
      961 gacccagaaa gcggcgcagg aagctcacga agcgatccgc cccacctctg cgtcgcgtga
     1021 tccggaatcc atccggcagt atctggagca ggaccaatac aatctctata agttaatctg
     1081 gaaccggttc atcgcctcgc agatggtgcc ggcgatttta gatgtgacgc gcgtcgagtc
     1141 gacgccgttc gaaacgaagg ggacatatct gttccgttcc accggcacca tagtgaaatt
     1201 ccccggccac accatcgtct atatggaagg tgtcgacaag gagctgtttg cgcagaagca
     1261 gaaaggcgat caggaagtgg aggatgatgc ggaacggcaa ttgcccgagt tgcatgaagg
     1321 ggagcggctg cgtcttgtcg aacaggccga gcaaacgatt cccggaatca cgtccaagca
     1381 gcattttacc cagccgcctc ctcggtataa cgaagcgctc ttgatcaagg aactggaaga
     1441 gaaagggatc ggccggccat cgacgtatgc cgcgatcatc tccaccattc aagaccgcaa
     1501 gtatgtggag aaggttgagg gacggttcat tccgactgaa accggccgca cggtgaacga
     1561 tttcttggtg aagggtttcc ctcaagtcgt gaacgtcgac tttacctcac acctggaaga
     1621 ggaactcgac gaggtagaag aggggaacaa accttgggtc gacgcggtac gtgatttcta
     1681 cgatccgttc acgcgagaga tggaacgggc caagacgatc cccggtccga aagacacagt
     1741 cgagccgccc acagatattc cctgtgaaaa gtgtggacgc atgatggaaa ttaaatgggg
     1801 gcgcaacggc aaatttctcg catgccccgg gtataaagag gacccgccct gtaagaacac
     1861 gcaaaacttc gagaaactcg ctgacggcac gatcaagatc gtaccgaaac aggaactgac
     1921 caccgatcag ctgtgtgaga agtgcaatag cccgatggtc gtaaagaccg gtcggttcgg
     1981 caagttcatc gcctgctctg cctatcctca gtgtaaaacc acaaaaccgt tagcactggg
     2041 ggtcaagtgc cctcagcctg gctgtggcgg cgatttggtg cagaaacgga cgcgcaaggg
     2101 ccggtcgttt tttgcctgca gcaagtatcc ggcctgtgag tacgccttgt gggatcgtcc
     2161 gatcaacaag gcctgtccga cctgcagtgc tccgttcctc atcgagaagg tcagcaagca
     2221 ggcaggccgc agcgtgcagt gccgaaacga agactgcggg tatcgcgaag ccggctaagt
     2281 ccttcgagat cgatctcctc tccgttgtct gccgaatccc tttcgatagt gagaatgctt
     2341 ttcatccgcc cgctgtgctt gccgctctca caggcggtgg cgcagttgac gaaacacgtc
     2401 ccgaaaccga taatgaaagc cgtacagatt gcgagcgacc tttccactaa ccaatgagcc
     2461 cctggcatga gggcctaccc gattgaagag agggggtaat catgaaagcc aaagaagggg
     2521 tcgtcaacat cctcaacaag atcctgacgg cggatctcac agccatcaat cagtatttcg
     2581 tccacgcca
//
LOCUS       DATIFR010000193         1138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_126682, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000193 DATIFR010000000
VERSION     DATIFR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126682"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          230 a          307 c          365 g          236 t
ORIGIN      
        1 cctcggatgg tgactcggat ggtgacttgt gaataatgct ccctgtatcc gttatagcga
       61 ggatggaagg aagagggtta ttcaaaaggc cctttgtcag catctctcga cccgcatcgg
      121 cgcaggtcag gggacgttag cgatcttggc aacggagttc tgaataagcc tctatgagca
      181 ttattcacaa gtcaccatcc gaggcggtag aactcgagcg cggtgatgat tcgggtgaca
      241 ttagggagct cggcgaggcg tccccggtag ccggtggcga ggatcttcca gttcttgagg
      301 tgtgcaatac accgctcgac agcggaccga atcgatgaga tggtcttgtt gtcggccttg
      361 tcgccgacgg agagctctcc acccggaggc ttcttgcggg gcgtggtcgc agacgttcct
      421 tggtatcccg ggtccgcgat gaccggggtg ttctcaaggg ccttttccca cccgcactca
      481 gcgaacgctg tacggtcatg aacggagcca cgcagcggct ccgagactcc cagcaggcca
      541 ccgctgtcat cggcggcaac ctggatgcta agcccggcac ggcgccgctg accccagtag
      601 ttgccgggtg accagcgtgg ttgccggtcg gcacgtcggt gccgtcgacc agcacgagcc
      661 ggccgcgcac ggcttcgacg gggtcgagaa catgtcggca cagcgcctgt ccgatcagcg
      721 gaaggaggcg cctatagatt ctggacacgg tcggttgact gactccgaac aggtcgccgg
      781 cagccatctg gttcaggttt tgccagacca tgacgagggt cagtacgacc atgcggtaca
      841 ggctcaaggc aggtggtcgt ccacacttgt gctcgggctg gacgatctgc aggatgcgtg
      901 ccacgagttc ctcggtttgg tccgcaggaa gccccgttgt agacgggtac cgcaacagcc
      961 ctcacctcgc aaaccgtgtt ctgttggaag aaacaacctt ctggtcgggg ctgttatctt
     1021 atattcgggt gatcgtggct tgtgaataat gctcatggaa gaaggagaaa caagaatgcg
     1081 agtagttact gcatttgtcg cagatacaga aagagatgat ggggaggtca gccgatca
//
LOCUS       DATIFR010000194        23459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2236062, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000194 DATIFR010000000
VERSION     DATIFR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236062"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VLD60_10995"
     CDS             <1..614
                     /locus_tag="VLD60_10995"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSB45531.1"
                     /translation="WSWILWTLDAKSYEKGTSPFVNQLGRQIVDERLTLQNRPSHSDL
                     LGTGFTQEGLPSEDALWIENGVLRQLAYDRFTAKMQGIKRIATLEAPCLSASGRTSHK
                     THDLIKQCERGILVTNFWYIRTVNPTDLTLTGMTRDGTFLVEDGQITSAVRNFRFHES
                     PLRVFKHVDAFTEPAEAVTSETGKLLVPAMVVRDFHFSSVTRF"
     gene            634..2355
                     /locus_tag="VLD60_11000"
     CDS             634..2355
                     /locus_tag="VLD60_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45532.1"
                     /translation="MSWIWLLHAVLLARPKQDTAPLAGPIMHERVNRPGRLVFLNSLA
                     MLVLATMMGTAQAAPPLFDNLGSLHHPISTGSELAQQYFDQGLRLVYAFNHEEAIRSF
                     EEAARHDPNAAMPYWGIALALGPNINAAMAKEDERRAIEAIKNARARLEKAGDAERAY
                     IDALTKRYISTKRATRSILDRAYADAMRTVWRKFPDDPDAGVLFAEALMDLRPWDLWT
                     AEGKPKPGTDEIVSTLEAVMAKYPDHPGACHYYIHAVEASPQPDRALACADKLAELMP
                     GAGHLVHMPAHVYVRVGQYHDSAERNVRAAQIDEQYLARYSPSGDYADAYYSHNLHFL
                     WASLMMEGRSAEALKTARVLMATITEEEARKDRIKEQYLPAHMFTLIRFGRWDEMLRE
                     PPPPKDLHLMEGIWRLGRGLALVATGRLPGGEGEQHALSGLTKQIRRTKSPEEKLERE
                     LLKIADRLLAGEIAAHRQRYDESLKAMKEAVKIDESLQYSEPPLWPLSTRHYLGAILL
                     MAGRAAEAESEYRVDLKRYPENGWALFGLIQSLKAQHKESEAADVTTRFEKAWAHSDV
                     ALTASRF"
     gene            2444..2977
                     /locus_tag="VLD60_11005"
     CDS             2444..2977
                     /locus_tag="VLD60_11005"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45533.1"
                     /translation="MRFVVGVMGPAKARRKDIENARMLGELIARREWVLLTGGRDVGV
                     MHAANQGAKKVARSLTVGVLPSTRDRVSKYVDVAIITEMGSARNNICVLSSQVIVACG
                     LGGSGTVSEVALALKAGKEVILLGATPVEEKFFKKLGGSLVSTVQTAEDAIDVILGRF
                     EQNDAQDLQAAQPWGRF"
     gene            complement(3093..3557)
                     /locus_tag="VLD60_11010"
     CDS             complement(3093..3557)
                     /locus_tag="VLD60_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HSB45534.1"
                     /translation="MIRWTGTLVLLLLTGCASWFIKGEPPDVLVANITPLDATAFEQR
                     LQVDLRIRNPNDFDLVITGIDFTLNVNGRRLARGLGNKEFTIPRLSDAVVSVQTSTST
                     FDIVRQVMSFSQKQDLSYNISGVLHSKEGRLPFDNAGLLLEQGQLSGQPAPR"
     gene            3660..4286
                     /gene="msrA"
                     /locus_tag="VLD60_11015"
     CDS             3660..4286
                     /gene="msrA"
                     /locus_tag="VLD60_11015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSB45535.1"
                     /translation="MTISRWTVTLLIAAFTGLGGGKVGLPEGHAADTGATAKAYFAGG
                     CFWCMEEAFEKVNGVLEVVSGYMGGTVKQPGYEEVSSGRTGHAESVEVRYDPAKVTYT
                     HLLEAFWRNVDPVTPNAQFCDHGNQYRSVIFYQGDEQRRLAEESKQAIEQSKRFTQSI
                     VTQIVAASEFYPAEEYHQDFYKKNPIRYKYYKFSCGRAQRLEVLWGKP"
     gene            4513..5049
                     /locus_tag="VLD60_11020"
     CDS             4513..5049
                     /locus_tag="VLD60_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB45536.1"
                     /translation="MIMAAALLVAVSPTTTQSDVENRHLYSKQEIRRAITFYAKRYRI
                     DPALLRAVIRAESGFRQDVVSRKGAIGLMQLTPDTAETLRVTDVRDSIQNIRGGAKQL
                     RRLLDLYGGDLPPALAAYNAGVHRVKGHKVPRIRETRRYVRRVLSYYHRAQAKQSSIL
                     TTKPGLQNPGRSTSFSKA"
     gene            complement(5118..5486)
                     /locus_tag="VLD60_11025"
     CDS             complement(5118..5486)
                     /locus_tag="VLD60_11025"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB45537.1"
                     /translation="MGIGESSSNRSAGQTGDSNVAERRGRRMALNRRLFFFGADEFEG
                     EATILDISTNGCRASSLTEVKVGMSLKLSLFLQDQQWPLRIDEALVRWVDGQTFGLEF
                     VSIRPVQRERLRGLIMKTKS"
     gene            5773..6933
                     /locus_tag="VLD60_11030"
     CDS             5773..6933
                     /locus_tag="VLD60_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB45538.1"
                     /translation="MGARRGNERMATLGHSEIRAMTKACVEVKGLNMAQGVCDTPAPA
                     VVLKAAAQAIEQGKNVYSRFDGLPELRQALAAKLSRDNGMKADPETEISVSAGATGAF
                     HASCLALLTPDDEVILFEPYYQYHISALQAVEAVPVIVKMHAPEWSLSADAIERAVTP
                     RTKAIIINSPGNPSGKVFSREELDEIARIAKRHDLFVFTDEIYEYFLYDGRRHVSIAS
                     LPDMAERTITIGGYSKTFSVTGWRIGYSVAADRWTQAIGAMNDLLYVCAPTPLQVGVA
                     AGIRELLPAFYRDMARDFEAKRDRFCAVLTKIGLTPSIPQGAYYVLADSTRLPGKTGK
                     DRAMHLLKTVGVAGVPGEAFFSGPDGARFIRFSYAKTDSDLDEACRRLARLD"
     gene            6986..7444
                     /locus_tag="VLD60_11035"
     CDS             6986..7444
                     /locus_tag="VLD60_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45539.1"
                     /translation="MPQSRPTLLLAGALVVLTTACAQPLVPRDASPQPPAPSSREVSD
                     DRRLLAGEWEYEDSAVVTLELDERGNGVYAWKEGRLETTALSNHTWQGMWFQKENDRE
                     GGFTVEFSEDFSEGEGRWWYSRIGPDHAPTQKGGTFHLTRKGSNAMSFAR"
     gene            7493..7813
                     /locus_tag="VLD60_11040"
     CDS             7493..7813
                     /locus_tag="VLD60_11040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB45540.1"
                     /translation="MESRKSPRVAVQLPMLFSGTGQAGDAIILNISREGCMVTAEHVA
                     APPAYVQLDMQLWEGQEPVHIELAAVRWASESRFGLEYIKLDSKQKERLTSFMAMLER
                     NSPA"
     gene            complement(7844..9595)
                     /locus_tag="VLD60_11045"
     CDS             complement(7844..9595)
                     /locus_tag="VLD60_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSB45541.1"
                     /translation="MQKTSRTSIEPRAFDPLIAEGFIRQADLIKAFQEAIDGNISLES
                     LLIDKYRIPKARLGRALSEFFDCPYVPFDDRLAVDHALMKDLSHDYLKNHHWIPLKRE
                     DQVLDVLIDNPHDIEKGGDIRRAFPGLTIRFSVSLQRDLEQFLSAAGGGSNVGSLSDT
                     LGELRSEARSEQEGADDIDSFNENDSAIVRLANQIIVEAYRKEASDIHIEPYSDRKET
                     AVRFRVDGTCFTAMRVPATYRRALVSRIKIMAHLDIAERRKPQDGKIRFKLGNDRQIE
                     LRVATLPTSGGNEDVAMRVLSAKEPLPLEAMEFEPGTLTSLLELAEQPYGLILCVGPT
                     GSGKTTTLHAVLKHLNTDERKIWTAEDPIEISQEGLRQVQVHPKIGFTFAAAMRSFLR
                     ADPDVIMIGEMRDMETANVAIEASLTGHLVLSTLHTNSAVETVTRLLDMGLDPFNFGD
                     AMLGVLAKRLCKRICLACKESYRPSHEEYDELARAYGIQAWEQLGIVYNDDFRLHRGR
                     GCEACNHLGFKGRVALHELFRNTEEIKNMIQGRAKTSEMQSLAMKSGMTTLLQDGIHK
                     VLAGSTTYKQIRSVAMK"
     gene            complement(9886..10776)
                     /locus_tag="VLD60_11050"
     CDS             complement(9886..10776)
                     /locus_tag="VLD60_11050"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB45542.1"
                     /translation="MKRAATIALLGTGLLGRAMAERLRAVGHAVIAYNRTATKALPLQ
                     VHGVTVVGTPELAVAQAEYVLLVLSDAAAIRSVLLSPACSAVLQGKTVIQMGTIASSD
                     SSALQRAVEGYGATYCEAPVLGSQAEATSGTLLVMVGATGEQFARVSPLLRSLGREPR
                     LIGPVGTAAAIKLALNQLIAAEISAFALSVGLVQRSGVSIDTFMAILRDSALFAPTFD
                     KKLPRLLKRDYHQPNFSTRHLLKDVELFLDEATDHGLQTGTLEGIRTILKDAIARGLG
                     DLDYSALYERINPPGNSSRT"
     gene            complement(10773..11579)
                     /locus_tag="VLD60_11055"
     CDS             complement(10773..11579)
                     /locus_tag="VLD60_11055"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HSB45543.1"
                     /translation="MMEQELTRLVETVKRAGARAREMATRGFEVQTKKDRSPVTTADL
                     EVNRILLDMQRVHFPEDGWLSEESPDKPDRLEKARVWIVDPIDGTKAYVNRLPEYCIS
                     VALVEQGKPVVAAIYNPSTDQLFTAIRGQGLSLNGKPVTLRPTEDATSLVMVSTREFR
                     SGRWAGLGGLVQCRPMFSIANALALVAAGQIHATITIEPENEWDLAAGVLLIEESGGA
                     VADAQGQPFRFNQKVPLFHGVIAVSATAGRELRPLLQAHADRARTKRTRS"
     gene            11718..12773
                     /gene="corA"
                     /locus_tag="VLD60_11060"
     CDS             11718..12773
                     /gene="corA"
                     /locus_tag="VLD60_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSB45544.1"
                     /translation="MKLVQKRSRKTGLPPGTLVHIGDKKADRVTIQAFLYAGAKCDEW
                     TVAQPEQLAPPSDESVIWVNVGGVHKLDVLDTFGKQFSLHPLLLEDIANTDQRPKLDD
                     YETYFFVVMKMLSLTERQDVMVEQVSLVLGRNFVLSFQENGTDVFHPVRERLRGGKGR
                     MRQAGADYLLYALVDAIVDQYFAVLESIGEKIEALQQVVVADPKPETLREIHALKRQL
                     LFLRRAVWPLRDVMNNLSRSDCLFLQHSTKVFFRDVYDHVVQIVDTIETLREMVSASL
                     DIYLSSVSYRLNAVMRVLTVITTIFMPLSFIASIYGMNFEYMPELKSQWGYPLVLAVM
                     TLVGVGMLFVFRSKRWL"
     gene            complement(12790..13176)
                     /locus_tag="VLD60_11065"
     CDS             complement(12790..13176)
                     /locus_tag="VLD60_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45545.1"
                     /translation="MNWSTIFFPVMWLWRPKLFFTLVFFIGAAYLLVAFNYSYSDGSR
                     AGYIQKFSKKGWFCKTHEGELAMTTVPGVAPVLWDFTVWDDRTAAQLTQVMGKRLVLH
                     YKEFRHLPTTCFGETAYFVDRVEVQE"
     gene            complement(13192..13815)
                     /gene="leuD"
                     /locus_tag="VLD60_11070"
     CDS             complement(13192..13815)
                     /gene="leuD"
                     /locus_tag="VLD60_11070"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSB45546.1"
                     /translation="MQAFTTLTGVVAALDRVNVDTDQIIPKQFLKTIKRTGLREGLFF
                     DWRKQKDGSPDPIFFLNQTRYHGASILLTRDNFGCGSSREHAPWALLDQGFRCVIASS
                     FADIFYNNCFQNGILPVVLKPDEVQTLMSTVLATPGYELTVDLGNQTVTTPSGTRYRF
                     DIDPFRKDCLYRGLDSIGLTLQHEAAIAAYESRRKAEAPWLFSDLRA"
     gene            complement(13823..15226)
                     /gene="leuC"
                     /locus_tag="VLD60_11075"
     CDS             complement(13823..15226)
                     /gene="leuC"
                     /locus_tag="VLD60_11075"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSB45547.1"
                     /translation="MAGQTLFDKIWGSHVVRAEPDGTTLLYIDRQLVHEVTSPQAFEG
                     LKLAGRRPRRPGATLAVPDHNVPTTDRRLGIADPVSATQIQTLEDNCREFGITLFGMN
                     DIRQGVVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEVRVDGTLSDRCSAKDIVLAIIGKIGTAGGTGYVIEYTGSAIRALSMEGRM
                     TLCNMSIEAGARAGMVAPDEKTIAYIKGRPMAPKGETFEQAVRAWQQLKTDSDAKYDT
                     SVTLRAEDIAPQVSWGTSPGMVIGVDGRVPDPRTIADEKTRKATERALEYMGLTANMP
                     ITEIRIDKVFIGSCTNSRIEDLRLAASLAKGKRVAKTVHAMVVPGSGLVKQQAEQEGL
                     DRVFTEAGFEWREAGCSMCLAMNADVLQPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAVEGHFVDIRNWG"
     gene            15369..15836
                     /locus_tag="VLD60_11080"
     CDS             15369..15836
                     /locus_tag="VLD60_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HSB45548.1"
                     /translation="MSLAPWLDPLIVQWSQWVLDSYRHWIGRELIERAGGAAFQSQTL
                     FEWPAVVVSHGTEADPILNYGNRAALDLWEMTWEQLVKTPSRLTAEPVNRAEREWMLE
                     QARSRGYIDTYRGVRISSAGRRFLVDNAIVWNVLDPQGQRLGQAATFSQWTEL"
     gene            complement(15816..16034)
                     /locus_tag="VLD60_11085"
     CDS             complement(15816..16034)
                     /locus_tag="VLD60_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HSB45549.1"
                     /translation="MIQPGRYRHYKGPDYEVLGLAKHSETEEEFVVYRALYGERGLWV
                     RPVAVFSEMIQVDGKQVPRFLRLELGPL"
     gene            16103..17464
                     /locus_tag="VLD60_11090"
     CDS             16103..17464
                     /locus_tag="VLD60_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB45550.1"
                     /translation="MIGGGSAGYAAARTARAAGADVAIVDHGPLGGLCILRGRMPSKA
                     LLRSAEVAALMRRANDFGLSPVDVRASLGAIMDRKNQLVQEFADYRIQQLRDPDFTLY
                     EARATFRSPHEIDAGGVALSAKAFIIATGSSSCEAAIAGLADAGFFTSDSILDLRTLP
                     TSLLVLGAGPVALELGQFFARLGTKVTILQRGETILSEMDEDVGQALEAALRHEEIEV
                     ITDAQLVRVTSDKGGKSVWVKQAGRERVFTGEAILQALGRRPNISGLQLDSAAVDVTA
                     GRIVTDEAMRTSQPHIFAVGDVNDLNPIVHLAIQQGEIAGYNATHPGAIPKEIDHRLD
                     AEVVFTEPQVAVAGLNERTCRTNEIPYLKASYPFGDHGKALCRGDLHGFVKLLAAPSN
                     GRLIGAQIVGPEAGELIHELMAVMYFQGTAADLLHIPHYHPTLAEIVTYPAEALVEQL
                     GAL"
     gene            17461..18138
                     /locus_tag="VLD60_11095"
     CDS             17461..18138
                     /locus_tag="VLD60_11095"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45551.1"
                     /translation="MSRRYLAAMGLLIVSLSACQQQTWESLMAAGQQAIQQGNYAEAE
                     RAFQAAVRKAEEFGLEDRRVAVSLSQLAQVYAGQGKHLEAEPVYLQALKIYQAVHGEN
                     HADVAATLNNLGVLHRMYGQYAEAEPLLTRALTIKERILGPDHPDVAVSLANLGQLWV
                     ARGQPDKAEPFYRRALAIRQKALGPSHPEVAKTLEDLANALRKLGRTEEATAFEIQAR
                     GIRANRS"
     gene            18171..18413
                     /locus_tag="VLD60_11100"
     CDS             18171..18413
                     /locus_tag="VLD60_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45552.1"
                     /translation="MPEAKEPSPINWRMNAMVARFVGIVAIALGFVLGMYGLDESGSH
                     WLQTALGLLVTGMLAQGYALYCSIKRMQQLQDRPKT"
     gene            18437..18844
                     /locus_tag="VLD60_11105"
     CDS             18437..18844
                     /locus_tag="VLD60_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45553.1"
                     /translation="MATTSLMVIVAGSLLACGGPWRDKYFKKGVDRLTQDEIAEKLGP
                     PHTAKTPALGGDTVWTYRVPLSDRDLNPMDLSGVTNAAQQAAALIGKPAADGPKPTLY
                     CYRYTLTFNEQKVLKQWKRDECVPGTRETLTTQ"
     gene            18883..19692
                     /locus_tag="VLD60_11110"
     CDS             18883..19692
                     /locus_tag="VLD60_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45554.1"
                     /translation="MPNIDSSIVLDSLKSLLLLLSILIIRTLIVRSISKNSALTMEAK
                     RRWVVSVRNTIVFVFLVGLVVIWAHELEAFAVSLVALAAALVLATKELILCWSGAALR
                     VGGKVYSVGDRIHIAGHRGVVLDHDIFTTKLLEIGPGSSSHLYTGRVAVFPNSLLFTN
                     ALVRENPDQEYGLYTLTIPLKAEDDWQKAEQRLLEAARIECASFKEEATRQMKLLEQT
                     NLLEAPSPEPRSTIQLSAPDRVQLVLRFPAPDRGRSRIEQAILRRFLLSSR"
     gene            19831..20271
                     /locus_tag="VLD60_11115"
     CDS             19831..20271
                     /locus_tag="VLD60_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45555.1"
                     /translation="MRKIAIGLFSAFCLLGAASLALADDAMKGEINIMKDQMKAETKG
                     MKAEAGATKEEMQGKMKAKGQETKAKAKAKQDEMKAGTKAKEKEMKAKANAHKDAAKA
                     KGDAMKAQGQEMKAAGQPMGQEMKTTGQDMKATGMEAAGAMTGK"
     gene            20476..21249
                     /gene="xth"
                     /locus_tag="VLD60_11120"
     CDS             20476..21249
                     /gene="xth"
                     /locus_tag="VLD60_11120"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSB45556.1"
                     /translation="MKIATFNANSLRKRLPIVLDWLEKYKPDVLCMQETKVQDSEFPL
                     LALAPSSYEITYRGMKSYNGVAVLSRKKPESVRAGLDDGGEADEPRLLHVVIDGIPII
                     NTYVPQGFEIDSPKYAYKLEWYKRLRRYFEKHLEPNRPAIWCGDMNVAPRPMDVHSPE
                     KHLTHVCYHEDARKAYEATVAWGFQDVFVKLYPDRQQFTFWDYRAPGSLETNKGWRID
                     HILATAPLAEKCNRVDVDVEPRRAKDPSDHTFLWAEFSV"
     gene            complement(21340..21510)
                     /locus_tag="VLD60_11125"
     CDS             complement(21340..21510)
                     /locus_tag="VLD60_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45557.1"
                     /translation="MTCQKCKGLMIAESRPDFSPEGTVMRCINCGLVLDPLIQQNRLM
                     KIHAKPATLHAA"
     gene            21630..22886
                     /locus_tag="VLD60_11130"
     CDS             21630..22886
                     /locus_tag="VLD60_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB45558.1"
                     /translation="MDILNDIAVIGAGAAGLAAGIFSGEGVRTGPLRIVVLDGAKTIG
                     AKILVSGGGRCNVTHDEVTAKDFFGNRHIIKNVLAAFSVQHTIQWFASMGVELKREET
                     GKLFPVTDKARTVLTALVDRCRKLGVAVQPDHRVTGIERLPGHKAGFLIHHAHGTLHA
                     KKVVMATGGRSIPKSGSDGFGYELVRRLGHHVTATVPALVPLVLDDRMFHQTLSGLSQ
                     DVELTTVVEGRTVDKRAGSLLWTHFGISGPVVMDASRFWSLAQEQKEKAELYGNFLPA
                     RTQEQARQWFVEQAGNHPRRALVKTLGQLIPERFAEALCLRVECDPQKAVAQLPREKR
                     DRLVAAVIKFQFPVDRDRGWNYAEVTAGGVPLNEINFRTMQSKLVPGLYLVGEILDCD
                     GRIGGFNFQWAWATGFVAGRAAAASL"
     gene            23004..>23459
                     /locus_tag="VLD60_11135"
     CDS             23004..>23459
                     /locus_tag="VLD60_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45559.1"
                     /translation="MIRFLGHILTSLGNKVLVALSGLGLAGFVVFHMLGNLQVFEGAN
                     ALNSYAAFLREMPILLWTARLGLLGVVSVHIGLAIRLVMRNRRARPVDYEVRDYRQAS
                     VASRTMALTGGLLLLFIIFHLLHLTLGVVDSSFTDRLDAHGRRDVFSKVI"
BASE COUNT         5032 a         6563 c         6960 g         4904 t
ORIGIN      
        1 tgtggagttg gatattgtgg acgctggacg ccaagtctta tgagaaggga accagcccct
       61 tcgtcaacca gctcggccgc caaattgttg acgaacggct gacgctccag aatcgtccca
      121 gccattccga cctgctcggg accgggttca cccaggaggg attaccgagc gaggacgcgc
      181 tctggatcga gaacggggtg ttgagacagc tagcctacga ccgatttaca gccaagatgc
      241 agggcatcaa gcgtatcgcc acgttagagg cgccgtgcct ttctgcctcc ggccggacct
      301 cccacaagac gcatgacctg attaagcaat gcgagcgcgg tattcttgtc acgaatttct
      361 ggtatatcag gacggtcaat ccgacggacc tgacgctgac gggaatgacc agagacggaa
      421 cgtttctcgt cgaagacggc cagatcacgt cggccgtgag aaacttccgg tttcatgaaa
      481 gtcctctgcg ggtcttcaag cacgtggacg cgttcaccga gccggctgaa gcggtcactt
      541 cggaaacggg aaagttgttg gtgccggcca tggttgttcg cgatttccac ttctcgagcg
      601 tcacaaggtt ctgacgggga ggccacacga atcgtgtctt ggatctggtt gttgcacgca
      661 gtcttattgg cacggccgaa gcaggataca gcgccattgg ccggtccgat catgcatgaa
      721 cgagtcaacc ggccgggtag gctcgtgttc ctgaacagcc tcgccatgct tgtccttgcg
      781 acgatgatgg gaacggcgca ggccgcaccc ccgctcttcg acaacctcgg ctctctccac
      841 catccgatca gcaccggatc cgaactcgcc cagcagtatt tcgatcaggg attaagactg
      901 gtctatgcgt tcaatcatga ggaggcgatt cggtccttcg aggaagcggc caggcacgat
      961 cccaatgcgg cgatgccgta ctgggggatc gccttggcgc taggaccgaa catcaatgcg
     1021 gccatggcca aagaggacga acgtcgtgcg atagaggcca tcaaaaacgc ccgagcccgt
     1081 ctcgaaaagg ccggcgatgc cgagcgggca tatatcgacg cgttgacgaa gcgatatatt
     1141 tcgacgaaac gcgccacgcg ctccattctg gacagagcct atgccgatgc gatgcggacc
     1201 gtctggcgga aatttccgga tgatcctgac gcgggggttc tcttcgcgga ggctctcatg
     1261 gaccttcgtc catgggatct ctggactgcc gaggggaaac cgaaacctgg cacggacgaa
     1321 atcgtctcga cgcttgaagc cgtcatggcg aagtatcccg accatcctgg cgcctgccac
     1381 tactacatcc atgccgtaga agcgtcgcct cagcctgatc gcgcactggc ctgtgcggac
     1441 aaacttgccg aactgatgcc gggagccggg caccttgttc acatgccggc gcacgtctat
     1501 gtgcgagtcg ggcaatacca tgactccgcg gagcggaatg tgcgggcggc tcagatcgac
     1561 gaacagtatc ttgctcgtta cagcccttcg ggcgactacg ccgacgccta ttacagtcac
     1621 aatctccatt tcttgtgggc ttccctcatg atggaagggc gaagcgcaga ggccctcaaa
     1681 acagcccgtg tgctcatggc aacgattacc gaggaagaag cccgcaagga tcgaatcaaa
     1741 gagcagtatc ttcccgcaca catgttcacg ttgattcgat tcggtcgatg ggacgagatg
     1801 ctacgggaac cacctccgcc gaaggacctg catctcatgg aggggatctg gcgattgggg
     1861 cggggcctcg cgctggtcgc caccgggcga ttgcccggtg gcgaagggga acagcacgcg
     1921 ctgtcaggcc tcaccaaaca aatccgacgt acgaagagtc cggaggaaaa actggagcgg
     1981 gagctcctga aaattgccga tcgtctgctc gccggcgaga tcgccgctca tcgtcaacgg
     2041 tacgacgagt ccctcaaggc aatgaaagaa gccgttaaaa tagatgagtc gctgcaatat
     2101 tcggagccgc cgctgtggcc tctctccacc cgtcactatc ttggggcgat cttgcttatg
     2161 gccggtcgtg cagccgaagc tgaaagcgag tatcgagtcg atttgaaacg gtatcccgag
     2221 aacggctggg ccctttttgg tctgatccag agtctgaagg cgcagcacaa agaatctgaa
     2281 gccgccgatg tcacgacacg gtttgaaaaa gcctgggctc acagcgacgt cgcgctcacg
     2341 gcctcccgtt tttgaccgct tcacctccca tgtttccggt tttgacaaac agaggagcga
     2401 accggctaca atccggcagc atgacgcgca ggaggcatcc atcgtgcgat ttgtcgttgg
     2461 agtaatgggg cccgcgaagg cgagacgaaa agacatcgag aacgcgcgaa tgctcggcga
     2521 actcatcgcg cgacgggaat gggtgcttct caccggcggc cgcgacgtcg gagtcatgca
     2581 tgccgccaat caaggggcga agaaagttgc acgaagtctc acagtcggcg tcctgccgtc
     2641 cacgcgggac cgcgtgtcca agtatgtgga tgtggccatc attaccgaaa tgggcagcgc
     2701 gagaaataac atctgcgtgc tgtcgagtca ggtgatcgta gcttgtgggc ttggagggtc
     2761 gggaactgta tcggaagtgg ctctggcctt gaaagcgggg aaagaggtca tcctgctcgg
     2821 tgccaccccg gtcgaagaaa agttcttcaa gaaactggga ggctcgctgg tttccacggt
     2881 gcagacggct gaagacgcaa tcgacgtgat cctcgggcgc ttcgaacaga acgacgcgca
     2941 ggacctgcaa gcggcgcaac catggggcag gttctaaccc cgctgcaact ttcctctcgc
     3001 ctctttatag aagaaaaagt gctggcctaa gaatgaaggg attggaccgc ttccttccgc
     3061 gttccgcgga acacggcggc ctgcatcatg cactagcggg gggctggttg gccggacagc
     3121 tggccctgtt cgagcaggag gccggcgtta tcaaacggca ggcggccctc cttgctgtga
     3181 aggacgccgg aaatattgta cgacaggtcc tgcttctgcg agaagctcat cacctggcgg
     3241 acgatgtcga aggtcgacgt gctggtttgc accgacacga cggcatcgct caagcgcggg
     3301 attgtgaact ccttattccc caggccacgc gccaaacgcc ttccattgac gttcagtgtg
     3361 aagtcgatac ctgtgatgac caggtcgaag tcattggggt tccggatacg gagatcgact
     3421 tgcaggcgct gctcgaacgc agtggcatcg agcggggtga tgttggctac cagcacgtcc
     3481 gggggctcgc ccttgataaa ccaggaagcg cagcccgtaa gaagcaggag aaccagagta
     3541 cctgtccacc ttatcatggt ggcgacatca tatcggaaca tccgacacaa agcgacggac
     3601 gtagattcat tgtaagatag gatgcacgag caagacagat gctgaggagg gatcccgcca
     3661 tgacgatctc acgatggacg gtcacgctgt tgatcgccgc tttcacaggg ctggggggag
     3721 gcaaggtcgg tcttcctgaa ggccatgcag ccgataccgg cgcgacggca aaggcgtact
     3781 ttgccggagg ctgtttctgg tgcatggaag aggcgttcga gaaggtgaac ggtgtccttg
     3841 aagttgtctc tggctacatg ggcgggacgg tcaagcagcc tggctacgaa gaggtctcat
     3901 ccggtcgaac gggtcacgcg gaatccgtgg aagttcgata cgatccggcg aaggtcacct
     3961 acacgcatct gctcgaagcc ttctggcgca atgtcgatcc tgtgacgccg aacgcgcagt
     4021 tttgcgatca cggcaatcaa tatcgctcgg tcatcttcta ccaaggtgac gagcaaagac
     4081 ggttggccga agagtccaag caggcgattg agcaatcgaa gcgattcaca cagtcgatcg
     4141 tgacgcaaat cgtggcagcc tcagagttct acccagctga ggaataccat caagatttct
     4201 ataaaaagaa ccccatccgc tataagtact acaagttcag ctgcgggcgc gctcagcgcc
     4261 tcgaagtgct ctggggcaag ccgtaagcga tcgcgaagcc tttgttggac ggatgtacaa
     4321 cctgacgcca aaagcagcca gactcgtcag tctttgaaca atcctcccgg tgcatctccg
     4381 taccaggctc tgggtatctc ctggatcatc agggacttgt agcgcacttt gtgtggcacg
     4441 gatgttgacc tttcaagagg gatgaccagg atcaagacca acgacgtccg aagccttttt
     4501 cgaggcagtt gcatgatcat ggctgcggct ctcctggtcg ctgtgtctcc gaccacgacc
     4561 caatccgacg tcgagaatcg tcacctttac tcgaaacaag aaatacgtcg cgcgattacc
     4621 ttttacgcga aacggtaccg aatcgatccg gcgttgttac gcgccgtgat cagggctgag
     4681 tcgggttttc gccaagacgt ggtttcccgc aaaggggcga tcgggctgat gcagctgacg
     4741 ccggatacgg ccgagacgtt acgcgtgacg gatgttcgcg attctatcca gaatattcga
     4801 gggggggcga agcaactccg tcgtctcctc gacctctacg gaggtgatct tccgccagcg
     4861 ctggcagcct ataacgccgg cgtgcatcgc gtgaaagggc acaaggtacc tcggattcgg
     4921 gaaaccagga gatatgtcag gagggtgctc agttattacc accgagctca ggcgaagcag
     4981 tcgtctatcc tcacaaccaa gccaggcctt caaaatcctg gccgttcgac gagtttctca
     5041 aaagcctaac cgcggttgtt tcggaaccag gaaatgcgat gagagaaggc ttggggcagg
     5101 ttgattccta aaggttgcta actcttcgtt ttcatgatga gaccacgtag ccgctcacgc
     5161 tggaccggcc tgatgctgac gaactcgaga ccgaatgttt gcccgtctac ccatcgcacc
     5221 aacgcttcat cgatgcgcaa aggccactgt tgatcctgca gaaacaaaga gagctttaaa
     5281 gacataccga ctttgacctc tgtgagcgag gaggcacgac acccgttcgt ggagatatca
     5341 agaatcgtag cttcgccctc gaattcatcg gctccaaaga aaaagagacg gcggttgagc
     5401 gccattcgac gacctcggcg ctccgccacg tttgaatcgc cagtctggcc agccgaacga
     5461 ttcgaggatg attcgccaat acccacagtg gcgtcctttc tacatagcgt ccttacagat
     5521 aaggatcttc accgccccat aacaatactg taggtgatag accgggtctc cgccttcccc
     5581 gagagcggta gccccaaaga gggatcgggg gactggttat gcagaaactc tagcataacc
     5641 ccgtcaaagc tactaacaaa aatcacgtaa gttgtatagg aaccatgggt tgtacgaacg
     5701 aaaccgctcc gagggggccg cttgaccttt tgggaagtcc tctgcgacaa aagcaaaaca
     5761 acggaggaat gcatgggggc taggcgagga aacgaacgga tggccacact gggccactcg
     5821 gagattcgtg ccatgacgaa ggcttgcgtc gaagtgaagg gcctcaatat ggcgcaggga
     5881 gtctgcgata ctcccgctcc cgccgttgtc ctgaaggcgg cggcgcaggc catagaacag
     5941 gggaagaacg tctattcgcg ctttgacgga ttgccggaat tgcggcaggc gctcgcagcc
     6001 aaattgtcgc gggacaatgg catgaaggct gatccggaga cggagatttc cgtaagcgcg
     6061 ggcgcgaccg gagcctttca tgcgtcttgt ctcgccctcc tcactcccga tgacgaagtg
     6121 atcctcttcg agccttacta tcagtatcac atcagcgcac tgcaggccgt cgaagcggtt
     6181 ccggtgatcg tgaagatgca tgcgccggaa tggtcgctct ctgcggatgc aatcgaacga
     6241 gccgtgaccc cgcggaccaa ggccatcatt atcaattcgc cgggaaatcc atccggaaaa
     6301 gtcttcagcc gagaagagct tgatgaaatt gcccggatcg ccaaacgaca cgacctgttt
     6361 gtcttcaccg acgaaatcta cgaatacttt ctctacgatg gccgccggca tgtgagcata
     6421 gcttccttgc ctgacatggc tgagcggacg atcaccatag gaggctattc gaaaacgttc
     6481 agcgtcaccg gttggcggat cggctacagc gtagcagccg acaggtggac gcaagccatc
     6541 ggggcgatga acgatctgct gtatgtctgc gcgccaacgc ccttgcaggt gggtgtggcg
     6601 gccggtattc gggagctcct tccagcattt tatcgtgata tggcgaggga ctttgaagcc
     6661 aagcgcgatc ggttttgcgc ggtgttaaca aagatcggcc tcacaccgtc gattcctcag
     6721 ggagcctatt atgtgctggc ggattcgaca agactgccgg gcaagacggg aaaagatcgg
     6781 gccatgcatc tcttgaagac cgtcggtgtc gccggtgtgc cgggggaagc gttcttttcg
     6841 gggccggacg gtgctcgttt catccggttc agctacgcca agaccgactc cgacttggat
     6901 gaagcctgcc gtcggcttgc aagattggac taatggtttc ttgtgatccc ttgtccaagg
     6961 gacgaagccg tgttatgaaa atcccatgcc ccagagccgc ccaacacttc ttctagccgg
     7021 agcccttgtc gtacttacga cggcctgtgc ccagccgctc gttccacgtg atgcgtcccc
     7081 gcaaccgccg gcgccatcct ctcgcgaagt atcggacgat cgccgcctgc tggccggtga
     7141 atgggagtac gaggacagcg ccgtggtcac actcgagctt gacgagcggg ggaatggagt
     7201 ctacgcgtgg aaagagggac ggttagagac gacggcattg tccaatcata cctggcaagg
     7261 tatgtggttc cagaaggaga atgaccggga gggcggattt acagtggagt tctcagagga
     7321 cttttcagaa ggggagggtc gttggtggta cagtcggatc gggcctgacc atgccccgac
     7381 tcaaaaaggc ggaacctttc acctgaccag gaaaggctcc aacgcaatgt cgtttgcccg
     7441 gtaatgacga tgagcgaacc gcccatctta cgacaggaag gagactagtc cggtggagtc
     7501 gaggaagtct ccccgggttg ccgtccagct ccctatgctg ttctccggga cagggcaggc
     7561 aggagatgcc atcatcctca atatttcccg ggaaggctgc atggtcacag cggagcatgt
     7621 cgctgcgcca ccggcctacg tccagctcga tatgcaactg tgggaaggac aagaacccgt
     7681 ccacattgaa ctggccgctg tgcgctgggc ttcagagagc agattcgggc tggaatatat
     7741 caaactggat tcaaagcaga aggaacgtct aacttcattc atggcgatgc tggaaaggaa
     7801 ttcgccggct taagcagcgg tgggttcgcg gacgagcagc cggctacttc atcgccaccg
     7861 atcgaatctg cttatacgtt gtggatccgg ccaacacctt atggataccg tcctgcagca
     7921 aggtggtcat tccggatttc atggcgaggc tctgcatctc ggaggtcttg gcgcggcctt
     7981 ggatcatgtt ctttatctct tctgtatttc tgaacaattc gtgcagcgcg acccttccct
     8041 tgaagcccaa atgattgcag gcctcgcatc ctcgccctcg atgtagccgg aaatcgtcat
     8101 tgtagacgat gccgagttgt tcccacgcct gtatcccata agcccgcgcc aattcgtcat
     8161 actcttcgtg cgatggacga tacgattcct tacaagcgag gcagattcgt ttgcacaaac
     8221 gcttggccag tacgccgagc atcgcgtcac cgaagttgaa cggatccaag cccatatcca
     8281 acaatcgagt cacggtttct accgcgctat tggtgtgaag tgtgctcaag accaggtgcc
     8341 ccgtcaatga cgcctcgatg gcgacgttgg ctgtttccat gtcgcgcatc tcgccgatca
     8401 tgatgacatc ggggtcggcc cgcaggaatg atcgcatggc ggcggcgaag gtgaatccga
     8461 ttttgggatg cacctggacc tgccgcaacc cctcttgtga gatttcgatc ggatcttccg
     8521 ccgtccagat ctttcgctcg tccgtgttga gatgtttcaa cacggcatgc aaggtggtgg
     8581 tcttgcctga tccggtgggg cctacgcaga gaataagacc ataaggttgc tcggccagct
     8641 ccagaagtga agtgagcgtg ccaggctcaa attccatggc ttcgagcggc aagggctcct
     8701 tcgccgatag cacacgcatc gccacgtctt cattgccgcc tgaagtggga agggtggcga
     8761 cgcgcagctc aatttgccgg tcgttgccaa gcttaaatcg tatcttccca tcctggggtt
     8821 tccgccgttc tgcaatgtcg agatgtgcca tgatcttgat gcgtgaaacc agtgcacgtc
     8881 gataggtggc tggaacgcgc atggccgtaa agcaggttcc gtcgacacga aagcgaaccg
     8941 cggtctcctt acggtccgaa tagggctcga tgtgaatgtc ggatgcttct ttgcggtagg
     9001 cttccacaat gatctggttt gccaatcgga caatagccga atcgttttca ttgaacgaat
     9061 cgatatcgtc cgcgccctcc tgttcggatc gtgcttcgct gcggagttcg ccgagcgtgt
     9121 cggaaagaga cccgacgttg ctgccgccac cggccgctga gaggaattgc tcaaggtcgc
     9181 gctgaaggct gacagaaaac cgaatcgtca ggccgggaaa agctcgccgg atatcccccc
     9241 ccttctcgat gtcgtgaggg ttatcgatga ggacgtccag cacctgatcc tcccgcttga
     9301 gaggaatcca atggtggttt ttcagataat catggctgag gtctttcatc aaggcgtggt
     9361 ccaccgccaa cctgtcgtca aagggtacat acggacagtc aaaaaactca ctgagcgctc
     9421 tccccagtct ggcctttgga atccgatact tgtcgatcag gagagattcg agggagatat
     9481 tgccatcgat ggcttcctgg aaggccttga taaggtcggc ttggcgaata aaaccttcgg
     9541 caatcagcgg atcaaaggct cgcggttcga tagaagtgcg actagttttt tgcatctggg
     9601 tgactggaaa agggagttgc tccgcaacgg gctaactcca tgtatcagat gaaggtgagg
     9661 gtactaacaa gccgctcaat cggcaaggaa attcacaatg atcagcatgc gattcgcgag
     9721 gaaagcacag aagtttggca gagaccagga gcggaaataa gaaccaggct tcggtcgatc
     9781 ggtggattca agaccttgaa cagagaagag tcgctgtggc acaaagattc caactcatgg
     9841 ccccgagcga tggcttttgc tcttccgggt cgcaaccgag gaactctagg ttcttgacga
     9901 attcccaggc ggattgatcc gttcataaag ggcagagtaa tcgaggtcac ccagaccccg
     9961 cgctatcgca tctttcaata tcgtccgtat cccttcgagc gtaccagtct gcagcccatg
    10021 atcagttgct tcgtccaaaa acagctcgac atccttgagc aggtggcgtg tcgagaaatt
    10081 cggctggtgg taatcgcgtt tcaacagccg gggcaacttc ttgtcaaacg tcggggcaaa
    10141 aagagcgctg tcgcgcagga tagccatgaa ggtatcaatg gagacgcccg accgttgcac
    10201 gagtccaacg ctcaacgcga aagccgagat ttccgcagcg attaattggt tgagagccag
    10261 cttgatcgcc gctgccgttc ctaccggtcc gatcaggcga ggctctcgcc cgagagatcg
    10321 caacagagga ctcacgcgcg cgaactgttc ccctgttgcg ccgaccataa cgagaagtgt
    10381 tcccgatgtt gcttcggctt gactgccaag gaccggcgct tcgcaatagg ttgccccgta
    10441 cccttcaacg gcgcgctgaa gcgcagagct gtcactcgac gcgatcgttc ccatttgaat
    10501 gacggtcttc ccctggagca ctgccgaaca ggccggtgac aacaggacgg agcggatggc
    10561 tgcggcgtct gataagacga ggagcacgta ctcggcctga gccacggcca gttcaggcgt
    10621 tcccaccacg gtgaccccat ggacttggag gggcagtgct ttggttgccg ttcgattgta
    10681 ggcgatcaca gcatgcccga cggcgcgcag acgttcagcc atcgcacgac ccaagagtcc
    10741 ggttcccagt aacgcaatgg tcgccgcccg tttcatgatc tcgtcctctt tgtacgggcc
    10801 cggtcggcat gggcttgaag cagtggccgc aattcccgcc ccgccgtggc ggagacggca
    10861 atgacgccgt gaaatagcgg gaccttctgg ttgaacctaa acggctgacc ctgcgcgtcc
    10921 gcaacggctc caccgctttc ctcaatcaac aggacgccgg cggccaagtc ccattcattc
    10981 tccggttcga tggtgatggt ggcatggatc tgtcccgcgg caacgagggc cagggcgttc
    11041 gcgatggaga acatgggacg gcattggacc aggccgccga gccctgccca tcggccactc
    11101 cggaattccc tggtgctgac catgacgagc gaggtggcat cctccgttgg ccgcaacgtc
    11161 accggcttgc cattcaggga gaggccctga cctcggatgg cggtgaacag ctggtccgtc
    11221 gatgggttgt aaattgccgc gacgaccggc ttgccctgct cgaccaatgc gacggaaatg
    11281 cagtattccg gcagccggtt tacataggcc ttggtaccgt cgatcggatc aacgatccag
    11341 acgcgggcct tctcaaggcg atcgggcttg tccggcgact cttcagagag ccatccgtcc
    11401 tcagggaagt gcacccgctg catgtcgaga agaattcggt ttacttcgag gtcggcagtg
    11461 gtgaccggcg agcgatcttt tttggtctgg acctcgaagc ctcgcgtcgc catttcacgc
    11521 gcccgagcgc ccgctcgttt caccgtctca accaagcgag tcaattcctg ttccatcatc
    11581 gcaagcctcg attgctcagc agccagggta gcaggtctgc cggctacgga aaagcggaag
    11641 tcttgggaca gtcccccgat tcccccttga ccgctagtgc gaacctgcgt acaagcaggg
    11701 agcggaggtc cgcatccatg aagctggtac aaaaacggtc gagaaaaacg gggctcccgc
    11761 ccggcacgct ggtgcacatc ggcgataaga aagcggatcg cgtcaccatc caagcgttcc
    11821 tgtacgccgg tgccaaatgc gatgagtgga cagtggctca gcccgagcaa ctcgcgccgc
    11881 cgtcggatga gtcggtcatc tgggtgaatg tcggcggtgt tcacaagtta gacgtgctgg
    11941 ataccttcgg aaaacaattt tccctccacc cgctcctcct cgaagatatt gccaacaccg
    12001 atcagcgtcc gaagctggac gactacgaaa cctatttctt tgtcgtgatg aagatgctct
    12061 cgctgacgga acggcaagat gtcatggtgg agcaggtcag tttggtgttg ggacggaatt
    12121 ttgtcctctc gtttcaagaa aacgggaccg atgtattcca cccggtgcga gagcggctac
    12181 gcgggggcaa agggcggatg cgacaggccg gcgccgacta tttactctat gccctggtgg
    12241 atgcgatcgt cgatcagtat tttgccgtgc tcgagtcgat cggagagaag atcgaagcgc
    12301 tgcaacaggt ggtcgtggcg gatcccaagc ccgagacgct ccgggagatc cacgccctga
    12361 aacggcagct cctgtttctc cggcgtgcgg tctggccttt gcgagatgtc atgaacaatc
    12421 tgtcccgatc ggactgtctg ttcctccagc actcgacgaa ggtcttcttt cgtgatgtct
    12481 atgatcacgt cgtgcagatt gtcgacacga ttgagacctt gcgcgagatg gtttcggcca
    12541 gtcttgatat ctatctgtcc agcgtcagct atcgattgaa tgccgtgatg cgggttctga
    12601 cggtcatcac gacgatcttc atgccgctga gcttcatcgc gagtatctac ggcatgaatt
    12661 tcgagtacat gccggagctg aagtcgcaat ggggttatcc cctcgtgctg gcggtcatga
    12721 cgctggtggg cgtggggatg ttgttcgtgt ttcgcagcaa acggtggttg tgacacgcgg
    12781 gcccgcgagt tattcctgta cctccacgcg atcgacgaaa tatgcggtct cgccgaagca
    12841 cgtcgtcggc agatggcgaa actccttgta gtgcaggacg agccgcttcc ccatcacctg
    12901 cgtcaattgc gcggcggttc tgtcatccca gacagtgaaa tcccagagca ccggcgccac
    12961 ccccggcacg gtggtcatcg ccagctcgcc ctcgtgtgtc ttgcagaacc agcccttctt
    13021 ggagaatttc tgaatgtagc cggcccggct gccgtcggaa tagctatagt tgaaagccac
    13081 aagcaagtag gcggcgccta tgaaaaacac gagcgtgaaa aacagcttcg ggcgccagag
    13141 ccacattacg ggaaagaaaa tggtggacca attcacgaga acctccttgg ctcacgcacg
    13201 aagatcggaa aacagccaag gagcttccgc tttgcgcctg gattcatagg cggcgatggc
    13261 ggcctcgtgc tgcaacgtca gcccgatcga gtcgaggcct ctgtacaggc aatctttccg
    13321 gaagggatca atgtcgaatc gatatcgggt gcctgagggg gtcgtcacgg tctgattacc
    13381 gagatcgacc gtcagttcat agcccggcgt ggcaaggact gtgctcatca acgtttgcac
    13441 ttcgtcgggt ttcaagacca ccggcaggat cccgttttgg aaacagttgt tgtaaaaaat
    13501 atcagcgaag ctcgacgcga tcacgcaacg gaagccttga tcgagcaagg cccaaggcgc
    13561 atgctcgcgc gaggacccgc aaccgaaatt gtcccgcgtc aacagaatgg aggctccgtg
    13621 atagcgggtt tggtttagaa aaaagatcgg atcgggagac ccgtccttct gcttccgcca
    13681 atcgaagaac aaaccttcgc gcagccccgt gcgcttgatc gtcttgagaa actgcttcgg
    13741 aataatctga tccgtgtcga cgttgacgcg atccagcgca gcaacgactc cggtgagggt
    13801 ggtaaatgcc tgcatggtga ctctagcccc agttccgaat atccacgaaa tgcccctcga
    13861 cggcggcggc cacggccatg gccggtgaaa ccagatgcgt tcgtccgccc gccccctgac
    13921 gcccctcaaa atttcgattg cttgtcgacg cgcaacgctc gcccggttgc agcacatcgg
    13981 cattcatggc gaggcacata ctacagccgg cctcccgcca ctcgaagcct gcttcggtaa
    14041 atacccgatc cagcccttct tgctcggctt gctgctttac caggcctgag cccggcacga
    14101 ccatcgcgtg gacggtcttg gcgacgcgct ttcccttggc caggctggcg gcgagccgga
    14161 gatcttcgat ccgcgaattc gtacaggagc cgatgaagac cttgtcgatt ctgatttcgg
    14221 tgatcggcat gttggccgtg aggcccatgt actccagggc ccgctcggtc gccttgcggg
    14281 ttttttcatc cgcaattgtt cgcggatccg ggacccttcc gtccacaccg atgaccatgc
    14341 ctggacttgt gccccagctt acttgagggg cgatgtcttc ggcccgcagc gtcacgctgg
    14401 tatcgtactt ggcatcggaa tcggttttca gctgttgcca agcccgcact gcttgttcga
    14461 atgtctcgcc cttcggggcc atggggcgtc ctttgatata ggcgattgtc ttttcatccg
    14521 gcgcgaccat tccagctctg gctccggcct cgatcgacat gttgcagaga gtcatacgac
    14581 cttccatgct aagcgcgcga atggccgaac ctgtgtactc aatgacgtat ccggttcctc
    14641 ccgccgttcc tattttgccg atgatggcaa ggacgatgtc cttggccgag caccgatcgg
    14701 agagcgtgcc atcgaccctg acctccatcg tcttgggtcg cttctgcacc aggcactgag
    14761 tggcgagcac gtgctcgact tcgctcgtgc cgatgccgaa ggccagcgcc ccgaatgcgc
    14821 catgcgtcga cgtgtgagag tcgccgcaga cgatggtggt tcccggcagg gtgaaccctt
    14881 gctctggtcc gatgacatgc accacgccct ggcggatgtc gttcatgccg aagagggtga
    14941 tgccgaattc ccgacagttg tcctctaagg tttgaatctg cgtcgcactg actggatcgg
    15001 caataccaag ccgccggtcc gtcgtcggga cgttgtggtc cggcacggcc agtgtcgccc
    15061 ctggtctgcg gggtcgccga ccggcgagct tcagtccttc aaaagcctgc ggggatgtca
    15121 cttcatggac taattgacgg tcgatataga ggagggtcgt gccatcgggc tccgcgcgaa
    15181 cgacgtggga cccccagatt ttgtcgaaca aggtctgacc tgccatgcgc actccttggt
    15241 gatctcaaag aggggagcag tcattataca gagttccccc atggtgttga caagagcgcg
    15301 attccacttc ttgcttcgcc ccaccggacg tgctatttgc cgcttggtcc gtacgggagg
    15361 ccgatgtcat gtctttagcg ccgtggctgg accctttgat cgtacaatgg agccaatggg
    15421 tcctcgacag ctaccggcat tggatcggtc gagaactgat cgagcgagcc ggcggggcag
    15481 cattccaatc tcaaaccctg tttgaatggc ctgctgtggt ggtttcgcac ggaacggaag
    15541 ccgaccccat cctgaactat ggcaaccggg cggccttgga tctatgggag atgacgtggg
    15601 agcaactggt gaagacaccg tcgcggctca cggctgagcc ggtgaatcgc gccgaacggg
    15661 aatggatgct ggagcaggcc aggtctcgcg gctacatcga cacctatcga ggtgtgagga
    15721 tttcttcggc cggccggcgc tttctcgtcg acaacgccat cgtctggaac gtgcttgatc
    15781 cccaggggca acgcctcggg caagcggcga cgttttcaca atggaccgag ctctagacgt
    15841 agaaaacgcg ggacctgttt tccgtcgacc tgaatcattt ctgaaaacac ggccaccggc
    15901 ctgacccaca gcccacgctc gccgtagagc gcgcgataca cgacaaactc ctcctcggtt
    15961 tcggaatgct tggccaagcc gagcacctca tagtctggtc ctttgtagtg tcgatagcgg
    16021 cctggttgga tcatggtgta tcctcttccc gcttcagtat agaaggaaga aacgtgggga
    16081 cacaacgcac acatgacgtc tcgtgattgg aggcggctcg gctggatatg ctgcggcgcg
    16141 gacggctcgc gcagccgggg cggatgtggc gatcgtcgat cacggccccc tcggcgggct
    16201 ctgcattctt cgaggccgca tgccgagtaa agcccttctg cggtcggccg aggttgcagc
    16261 cctcatgcgc cgcgccaacg actttggcct ttctccggtt gatgtgcggg caagtctcgg
    16321 cgccatcatg gaccggaaaa atcaattggt tcaagagttc gccgactacc ggattcaaca
    16381 gctacgggat ccggacttca cgctttacga agccagggcg accttccgct cccctcatga
    16441 gatcgatgcc ggtggtgtcg cgctttcggc gaaggccttc attattgcga ccggttccag
    16501 ctcctgtgag gcggccatcg ccggtttggc ggacgcaggc ttcttcacca gcgattcgat
    16561 tctggacctg cgcacgctcc ccacgtcatt actcgtcctg ggggcgggac ctgtcgctct
    16621 cgaattaggc cagttttttg ccaggctcgg cacgaaggtg acgatcctcc agcggggaga
    16681 gaccatcctg tcggagatgg atgaggatgt cgggcaagcc cttgaagctg ccttgcgcca
    16741 tgaggagatc gaggtcatca ctgatgcgca gttggtccgc gtcacttccg acaagggagg
    16801 caaatcggtc tgggtgaaac aagccggccg ggaacgggtc ttcacaggtg aagccattct
    16861 ccaggcgttg ggccgtcgtc ccaacatttc cggtctgcag ctcgattctg cggcggtcga
    16921 cgtgacagcg ggacggatcg tgaccgacga agcgatgcgc acgtcgcagc cgcacatttt
    16981 tgcggtggga gatgtgaacg atttgaatcc gatcgtccac ctcgccattc aacagggaga
    17041 gattgccggt tacaatgcga cgcatcctgg agcgattccc aaggaaatcg atcatcgcct
    17101 tgatgcggag gtggtattta cggaaccgca ggtcgctgtt gccggcttga acgagcggac
    17161 gtgccggacg aacgagattc cgtatctgaa ggcgtcttat ccgtttggcg atcatggcaa
    17221 ggcgctgtgt cggggcgatc tgcacggctt cgtcaaattg ttggcggctc cttccaacgg
    17281 gcgattgatc ggcgcacaga ttgtgggtcc tgaagcagga gaattgatcc acgaactcat
    17341 ggccgtgatg tattttcaag gcacggctgc cgatctcttg cacatcccgc actatcatcc
    17401 gacgctggcg gaaatcgtca cctatccggc cgaagcactg gtggagcagt taggagcctt
    17461 atgagtcgcc ggtatctcgc tgcgatgggc ctgctcattg tgtcgctgtc ggcttgtcag
    17521 cagcagacat gggagtcgtt aatggctgca ggccagcaag ccatccaaca ggggaactat
    17581 gccgaggcgg aacgggcgtt ccaagcggcg gttcggaagg ccgaggaatt cggcctcgag
    17641 gatcgccgcg tagcggtgag cctttcacag ttggcgcaag tctatgccgg gcaaggaaag
    17701 cacctggaag ccgaaccggt ctatctccag gcgctcaaga tctatcaagc tgttcacggg
    17761 gaaaatcatg cagatgtggc ggcgacgctg aataatctgg gagtgctcca tcgcatgtat
    17821 ggacaatatg cggaagccga accgctgttg acgagggcac tgacgatcaa ggagagaatc
    17881 ttggggcccg atcatcccga tgtggcggtg agcctcgcca atctgggtca gttatgggtg
    17941 gcgcggggac agccggacaa agccgaaccg ttctatcgac gcgccctcgc gatccggcaa
    18001 aaagcccttg gaccgtctca tcccgaggta gccaagactc tggaggattt ggccaatgcc
    18061 ttgcgaaagc tcggccgaac ggaggaggca accgcgttcg aaatccaagc tcgcgggatt
    18121 cgagccaacc ggagttgaac cagccccgcc ttccgttgta gcgtagcatc atgcctgaag
    18181 cgaaggagcc tagtcccatc aactggcgca tgaatgcaat ggtcgcccgc tttgtcggga
    18241 tcgttgcgat cgcgctcgga ttcgtcctgg ggatgtatgg tctggacgaa tcgggttcgc
    18301 attggctaca gacagcgctg gggctgctgg tgacggggat gttggctcaa ggctatgccc
    18361 tctattgttc aatcaagcgc atgcaacaac tgcaagatcg acccaagaca tgatgccttc
    18421 cagctggatt cctcgtgtgg ccacgacatc gctgatggtg atcgttgccg ggtcgcttct
    18481 cgcctgcggc ggcccctggc gcgataaata tttcaagaaa ggggtcgatc gcttgacgca
    18541 ggacgagatc gcggaaaagc tcggcccgcc ccatactgcg aagacccctg cgctcggtgg
    18601 cgatacagtc tggacctatc gcgtgccgtt aagcgacagg gacttgaatc ccatggatct
    18661 gtccggcgtc accaacgccg cgcaacaggc ggctgctttg atcggaaagc ctgcagccga
    18721 cggacccaag cccacattgt attgctatcg gtatacgctg accttcaatg agcagaaggt
    18781 ccttaaacaa tggaaacggg acgaatgcgt tcccggtacg cgcgagacct tgaccaccca
    18841 ataatcacct acgcttcagt tgcatgacct atgcctgcat ggatgccgaa catcgatagt
    18901 tccatcgtgc tcgacagcct caagtcgctc ttgctgttgc tctcgattct gatcatccgc
    18961 acgctgatcg ttcgttcgat ctccaagaat tccgccctca ccatggaagc caagcggcgg
    19021 tgggtagtat ccgtgcgcaa tacgatcgtc tttgtctttc tggtcgggct ggtcgtgatc
    19081 tgggcccatg aactcgaagc ctttgcggta tcactcgtcg ccttggccgc cgcgctcgtg
    19141 ttagcgacga aagagctgat tctctgttgg agcggggccg cattacgcgt gggagggaaa
    19201 gtctattcag tgggtgatcg gattcatatt gccggtcatc gcggtgtggt gctggatcac
    19261 gatattttca ccaccaagct cttggaaatc gggccaggtt cttcgtcgca tctctacacc
    19321 ggtcgtgtgg cggtgtttcc caacagcctg ctctttacga atgcactggt cagagaaaat
    19381 ccggaccagg agtacggtct ctatacactg accatcccgc tcaaggcaga agacgattgg
    19441 cagaaggcgg agcaacggct actcgaggcg gcgaggatag aatgcgcttc cttcaaggag
    19501 gaggcgaccc gtcagatgaa gctcttggaa caaacaaatc tactcgaagc gccctctccc
    19561 gagcctcgga gtaccatcca gttgtcggca ccggatcgcg ttcaactcgt attgcgcttt
    19621 cccgctcccg accgaggtag gtctcgcatc gaacaggcaa tcctccgccg gtttcttcta
    19681 tcttcccgct gactcttccg gcctgatacg tctgtccttt tggaattttc gcgtcttgac
    19741 aggggaccag agtcgtgcaa cgctggccgt gtcccgatgc aaactacaaa tctcctttgc
    19801 gattaccctt aaataatgag gaggtgccct atgcgcaaga tagctattgg cttgttttcc
    19861 gcattctgcc tgctcggtgc cgcgtcgttg gccttggccg acgacgccat gaagggcgag
    19921 attaacatca tgaaggacca gatgaaggcc gaaacgaaag gtatgaaggc cgaggctggc
    19981 gcgacaaaag aagaaatgca gggcaagatg aaggcaaaag ggcaggagac gaaagccaag
    20041 gccaaagcca aacaggatga gatgaaggct ggcacgaagg cgaaagagaa agaaatgaag
    20101 gccaaggcca acgcccataa agatgcagcg aaggcgaagg gcgatgcgat gaaagcgcag
    20161 ggtcaggaaa tgaaagccgc tggtcagcct atgggtcagg aaatgaagac aaccggtcag
    20221 gacatgaaag ctaccggcat ggaagctgct ggtgccatga cgggaaaata gtctcgcaca
    20281 ccacttaaaa ggagtaggac acggcccgtt ggggggggag atctcccaac gggccgttga
    20341 atatcatgcg cactgaaatg agatcacgac agcggaagac agacgagtgc gggcctagag
    20401 attggacaac cgcttcgctt gttgctgctc ctcgcttttg ctaggatgcc gcggttcgat
    20461 gtctggagtg cgcgcatgaa gatcgcgacg tttaacgcca attcgctgcg taagcgtttg
    20521 cccatcgtcc tcgattggct ggaaaagtac aaacccgacg tcttgtgtat gcaggaaacg
    20581 aaggtgcagg acagtgaatt cccgctgctt gcgctggccc cttccagcta cgagatcacc
    20641 tatcgcggga tgaagtccta taacggagta gcggtgttga gtcggaagaa acctgaatcg
    20701 gtgagggccg ggctcgacga cggcggcgag gcggatgaac caagactgct gcacgtggtc
    20761 atcgacggga ttcccattat taacacctac gtccctcaag gattcgagat tgactcaccc
    20821 aagtacgcct acaaactcga gtggtacaag cgcctccgtc gatattttga gaaacacctc
    20881 gagccgaaca ggccggctat ctggtgcggc gatatgaacg tggcacccag accgatggac
    20941 gtacatagtc cggagaaaca tctgactcac gtctgctatc acgaagatgc cagaaaagcc
    21001 tatgaggcaa cggtggcttg gggatttcag gacgtgtttg tgaagctcta tccggatcga
    21061 cagcagttca cgttctggga ttatcgagct cctggctcgc tggaaacgaa taaaggctgg
    21121 cgcatcgacc acattctcgc gacggccccg ctggcggaga aatgtaacag ggtagacgtg
    21181 gacgtcgagc cgcggcgagc gaaagacccc tcagatcata cgttcctatg ggcggagttt
    21241 tcggtctgag ccacgtgcgc aacgtccaca cctgcccggt ccggtccgtg cggcaagtcg
    21301 tccccacaaa ccggacgggc tatttacccc ggcaacccgt tatgccgcat gcaacgtggc
    21361 cggctttgcg tgaatcttca tcaggcgatt ctgctgaatc aagggatcga ggaccaggcc
    21421 acagttaatg cagcgcatca cggtaccctc aggtgaaaaa tccggcctcg actccgcgat
    21481 catcaaacct ttgcatttct gacaggtcat ctgtcttcct ccaatttccc ggttcatcca
    21541 tacatgaacg tttggcgatt cgggggacaa tgtagcagtg aaagattaac ggggtgttag
    21601 cgggcagtta aaaatctcta atgtctacca tggatatatt gaatgatatc gcggtgatcg
    21661 gggccggtgc ggcaggcttg gcggcgggaa tcttttcggg ggaaggggtg cggacgggac
    21721 cactccgtat tgttgtgctc gacggcgcca agaccattgg cgccaagatt ctggtgtccg
    21781 gtggaggccg ctgcaacgtc acacatgacg aggtgacggc aaaagacttc ttcggcaatc
    21841 gccatatcat taagaacgtg ctggctgcgt tttctgttca gcatacgatt caatggtttg
    21901 cctcgatggg cgtcgaactg aagcgggaag agaccggcaa gctttttcct gtcaccgata
    21961 aggcccggac cgttctgact gcgcttgtgg atcgctgtcg aaaactcggt gttgccgttc
    22021 agccggacca tcgagtcacc gggattgagc gtctgcctgg tcacaaagcc ggattcctca
    22081 ttcatcatgc acacggaacc ctgcacgcga agaaagtcgt tatggcgacc gggggccgat
    22141 cgatccccaa atcggggagc gacgggtttg gctacgaact ggtacgacgg ctgggtcatc
    22201 acgtgaccgc cacagtccca gcgctcgtgc cgttggtgct cgacgacagg atgttccatc
    22261 agacgctctc cggtctctcc caggatgttg agctgacgac ggtggtagag gggcgcacgg
    22321 tcgataagcg agccggaagt ctgctctgga cccacttcgg aatcagcggc cccgtcgtca
    22381 tggacgcgag ccgcttttgg tcgttggcac aggagcaaaa ggagaaggcg gaactctacg
    22441 gcaatttcct tccggccagg acgcaggagc aggcgaggca atggttcgta gagcaagccg
    22501 ggaatcatcc tcgccgcgcg ctcgtgaaaa cactgggcca gctgataccg gaacgctttg
    22561 ccgaggcgct ctgccttcgt gtcgaatgcg atccacagaa agcggtggcg cagcttccac
    22621 gggagaaacg ggatcgcctg gtcgcagccg tcatcaagtt tcagtttcct gtcgaccggg
    22681 atcgaggctg gaattatgca gaagttacgg ccggcggcgt gccgctcaat gaaatcaatt
    22741 tcaggacgat gcaatcgaaa ctggttccag gattgtattt ggtcggcgag atcctcgact
    22801 gtgacggccg catcggaggt tttaactttc agtgggcctg ggcgacgggg tttgtcgccg
    22861 gtcgagcggc tgcggctagt ctgtagtcgc ggatgacgat aatttcccgt cggcgagacg
    22921 atcacttccc atcgcgcccc ccttgaggtc tacaaaggag cagagtacaa gagaggcgag
    22981 agcgtcgatt tggagcatcg ggcatgatcc gcttcctcgg acacattctc acttcgctcg
    23041 gcaacaaagt attggtggcc ttgtcgggac ttggccttgc cggctttgtt gtgtttcata
    23101 tgctcggcaa cctacaagtc tttgaagggg ccaatgccct gaacagctat gccgcttttc
    23161 ttcgggaaat gccgatcctc ttatggacgg cccggctggg gctgctcggg gtggtgagcg
    23221 tgcacatcgg cctggctatc aggctcgtga tgcgcaatcg acgagctcgt ccggtggact
    23281 acgaggttcg cgactatcgt caggcctcgg tggcatcgcg caccatggcc ctgacaggcg
    23341 gcttgctcct actgttcatc atcttccatc tcttacacct tactcttggc gtggtggact
    23401 cctcttttac ggatcgactg gatgcgcacg gacgtcggga cgtgttcagc aaggtcatt
//
LOCUS       DATIFR010000195         1114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_899177, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000195 DATIFR010000000
VERSION     DATIFR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899177"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VLD60_11140"
     CDS             <1..615
                     /locus_tag="VLD60_11140"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB45560.1"
                     /translation="IGRLETDGRISAVADHYNGYRINSPNDLVLKSNGDLYFTDPPFG
                     LPNAFDDPDKAPVQGVYRVSKDGTVTRLIADIKAPNGIAFSPDEQTLYVSDVDPRRAA
                     WLAYDVKQDGTVTNGRVFFDATRWRKDPFFGPDGFKIDQHGNIFGARPGGLSVIAPDG
                     TLLGTIDTGQPTSNVAWGEDGQTLFITGGSSVYRVRLATKGAAY"
     gene            690..>1114
                     /locus_tag="VLD60_11145"
     CDS             690..>1114
                     /locus_tag="VLD60_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSB45561.1"
                     /translation="MADQPHNHAQPLSGLEPDAALLAILEGTAAETGEGFFAALVENL
                     AKALGTHGAWVTEYFPETRRLRALAFWMDGQWVKDYEVDLAGTPCERVIDTAQLVHFP
                     DRILEIYPDEEDLRKSGAVSYMGVPLQDTDGRVLGHMAV"
BASE COUNT          239 a          337 c          318 g          220 t
ORIGIN      
        1 attggtcgat tggaaacaga cggcagaatc agcgctgtgg cagaccacta caacggctat
       61 aggatcaaca gcccgaacga tctcgtcctc aaatccaatg gtgatctgta cttcaccgac
      121 cctcccttcg ggctcccgaa cgccttcgat gatccggata aggcaccggt ccagggtgtc
      181 tatcgcgtgt cgaaagacgg aacagtcacg cgcttgattg ccgacatcaa ggcaccgaac
      241 ggaatcgcgt tttcacccga tgagcaaacc ctctatgtgt ccgacgtcga tccaaggcga
      301 gcagcctggc tggcctatga tgtgaagcag gacggcacgg tcaccaatgg ccgtgtcttt
      361 ttcgatgcca cccgatggcg caaggacccg ttcttcgggc cggatggctt caagatcgat
      421 cagcacggca acatctttgg agctcgcccg ggcggactca gcgtgatcgc ccccgacggc
      481 acactcctcg gcacgatcga caccggccaa ccgacgtcga acgtggcctg gggcgaagat
      541 ggacagactc ttttcatcac gggaggctct tcagtctacc gcgtgcgatt ggccacgaag
      601 ggtgccgcct attaggtccg tgcacgaaca tcgattgccc tcctcttgcc tccgtttcat
      661 tccctggctc atacttggcg tacgagtcta tggccgacca accccataac catgcacagc
      721 cattgagcgg tctggaaccc gacgccgccc tcctcgccat tctggaagga acagctgcgg
      781 aaacgggaga gggatttttc gccgcgttgg tggagaacct ggcgaaagcg ctcggcacac
      841 atggagcttg ggtcacggaa tactttccgg aaacacgccg cctccgggcc ctggcgtttt
      901 ggatggacgg tcaatgggtg aaggactacg aagtcgatct cgccggcaca ccctgcgaac
      961 gcgtgatcga cacggctcaa ttggtgcatt tccccgatcg gattctcgaa atctatcccg
     1021 acgaagaaga cctcagaaaa tccggagccg tcagttatat gggcgtgccg ttgcaggaca
     1081 cggacgggcg cgttttgggg cacatggcgg tcat
//
LOCUS       DATIFR010000196        18736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2239727, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000196 DATIFR010000000
VERSION     DATIFR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239727"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VLD60_11150"
     CDS             <1..607
                     /locus_tag="VLD60_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSB45562.1"
                     /translation="SGGLRAAGRRDRINIMKVPLSKGIFLIAAPNLRDPNFRQTVVLL
                     CEHGAEGALGVVVNRPTAMSISEALPQVPVLEGQRHMLFSGGPVQPNQVMLLYRLNQL
                     PENSHHVFDGVCLGGDMELVDKILTTDQGREAFRAYVGYSGWGPGQLESEMKTGSWIT
                     LPADPSFVFEKDPTRVWSEILSTLGEEYRLYADMPFDPSLN"
     gene            661..1911
                     /locus_tag="VLD60_11155"
     CDS             661..1911
                     /locus_tag="VLD60_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HSB45563.1"
                     /translation="MPTQPTAHSSQRPALILIIFGLTGALSACGVAYTIVKSESKLDK
                     LAVPMTKAQVVDEIGRPDRVLRDDGRLLVWEYSLTARRQWLYELGLCPVSIWIGGCII
                     YPFTNVAMEHQREYPFHVVLVNEELCAWGTPAAILQRRRACAATGVPIGLSGGARGRP
                     EPIVSSNGPINRDNIGRYRTMAVMLFEDAPGSSGSGSRVAGIVTTLLLDLDIHMVERA
                     KLDEVLKEQVIQLTHADDANVLKVGRLVGAQAIIVGEVQQWERHEQERMHSVSLSLRI
                     IDVETGLLLFSGEGHLADSTTDDPESSARLIAHRILARFGSQTGLLGSGRIGVNWELL
                     EEKGGYFYLVRELRSGLPAEKGGLKVGDRIMACNGASLSAVKAEREAKRLCQVEAGQT
                     LLLDIQRAGCPMQIELTADKRPGL"
     gene            1949..3334
                     /locus_tag="VLD60_11160"
     CDS             1949..3334
                     /locus_tag="VLD60_11160"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HSB45564.1"
                     /translation="MDRSETPQAGSDTLIKKLHERPQIPLADWLRAPAHVHYMAFRMA
                     DPPEQRKASREEFRSLLSAFAIEPAKTVLRETFGYGVKEAGTGDRLIVVWQAHTEYYN
                     YQIWHLPGPSNPALSFGSISFPDYKFPVTPLGTEVCRLDILLTAESLPSRERMRTLFP
                     GPMLYGSKIFDDQTSLVTSFTPDEQGRERYWVGLGAPQASASRLKDIVDAIMRIETYY
                     HLLLMQKPLFSAAIDQVYKFEQVHLKQREIISGHIAHADSQALQRWLNSLTQDLLKTS
                     RIAGKLHFELSASLPYDKIVHATLASLAERPLESYRPVSDYVLSGITGVADGYQQLLK
                     RIETLRSGFEGIISIIRTRVDLIVEGQNLTLLQSVDKTTRSQVILQHTVEGLSVIVIA
                     YYLAGLAGYVFKGFSEMGWLRNAHIASAVFVPIAIGLAFVITTVSKKYLHKKWSGEQP
                     PTEGSKTPPPQ"
     gene            complement(3398..6187)
                     /gene="uvrA"
                     /locus_tag="VLD60_11165"
     CDS             complement(3398..6187)
                     /gene="uvrA"
                     /locus_tag="VLD60_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSB45565.1"
                     /translation="MPQPVAPTPASNDLIIEGARQHNLKNISLKIPHNKVTAITGLSG
                     SGKSSLAFDTIFAEGQWRYVESLSTYARMFLEKVARPDVDRLLNVRPAIAIEQKNQVR
                     TARSTVGTTTEIADLLRLLFAKIGTPICPDCHQKARSFHPDSVADDLLIRFPDARAMV
                     LFAVAAPAPTLEHTFVRSLLTRGFTRLKAGDEILDLHNAMLPSFHKSEALHVVLDRVV
                     VREDNRSRLVEAIETAFLEGEGRCAVDVIGHGLESYSTSFLCQRCSRTFEPLRPILFS
                     FNHPLGACPECKGFGNVLRYDPELVIPDHNKSLAQGAIEPWSKPGTDWWQKQMLLAMK
                     RRDVDITVPFKNLPKPTQQLLWEGDKSFDGINDFFEYMEGKRYKLHVRVLLSRYRTPF
                     NCPICNGSRLKPDARFVKIAGHDIHQITELTVEQLASWLDQLSLQPFERDIASDILRQ
                     LQAKLSFLLRVGLGYLTLARQTKTLSGGEAQRVSLANQLGARLVGTLYVLDEPTIGLH
                     ARDTDLLAGILKDLAAVGNTVVVVEHDRNMIESADFIVELGPQSGEKGGEIVCAAPAK
                     EFLQDRRALTARYLRGEEEIPLPRSRRSGNGKVLVIAGAGEHNLKDLVVRFPLGMLIC
                     VTGVSGSGKSTLVEDTLYRALARAFRVESLPMGRFKAIKGIEHLNGVRLIDQQPIGRT
                     PRSNPITYLKCFDEIRQLFASERDALRQGFSPGHFSFNAAGGRCERCEGSGVEKLEMY
                     FFEDIYAPCEICEGMRFKPEVLAIRYRRKTISDVLTMTVDEALTFFTGTPRLQERLHL
                     LSSIGLGYLRLGQPATTLSGGEAQRLKVAAELKDSSAKNMLYILDEPTTGLHFDDVKK
                     LLAVLHKLVNAGNTLVVVEHNLDVIKAADWIIDLGPEGGDAGGQIVAEGRPEQVAKVG
                     TSHTGRFLAQVFSNT"
     gene            6435..7013
                     /locus_tag="VLD60_11170"
     CDS             6435..7013
                     /locus_tag="VLD60_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45566.1"
                     /translation="MTQSSVPTDYLGIGRQLAVLLGSSLEGPSGEALKELARAYDPSA
                     NESRISAEVFLFHKYLIVQTCVGMFPEAQVERVIGGLFAALNERATGLEFSQERQNAM
                     EQMWQMRAKQFDRPFTQDREHFLDESSDGIYWKRSISRFCQNVREIENPPDIWAGGKG
                     PSHKASTTVTETIDHLVSAMSEMSRLHFGSPA"
     gene            complement(7100..7627)
                     /locus_tag="VLD60_11175"
     CDS             complement(7100..7627)
                     /locus_tag="VLD60_11175"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB45567.1"
                     /translation="MISLLEFVTITGMATLSAAGVSHGLNLLRQKRGDNSRVPTRSSN
                     HDVASGRPSAGAWHEPRYGKRHQVNCRMEYLAGHNRAEGILLDLSRQGWRARGTQPVA
                     KGTALAVYIYFPDLAQPIMVDEAVVRWTDGLEFGVEVTRITPEAASKFSDYLSINHPA
                     QKSTPEYALSHFSYS"
     gene            complement(7834..8172)
                     /locus_tag="VLD60_11180"
     CDS             complement(7834..8172)
                     /locus_tag="VLD60_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HSB45568.1"
                     /translation="MHDVVKYTVYFLLGGAIVSLSTYLGSQGRSFLAAFASTFPAITG
                     ATFVLLYLNGGSDSLVGYARNLLWFVPPWIVYVTAMIVGVPRLGFWPTMAISLTLYMG
                     CVGLVKLAIR"
     gene            complement(8185..9204)
                     /locus_tag="VLD60_11185"
     CDS             complement(8185..9204)
                     /locus_tag="VLD60_11185"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45569.1"
                     /translation="MNPSFSSYLVNDVFGDPGVYVEIRWSRRALLFDAGHNDSLGPTR
                     LLRANDIFISHTHMDHFIGFDAVVRVALGRGKKLRLYGPPGLIDNVQGKLRGYTWNLV
                     DGYPLSIEVQEFHGQQVRQAFFHATNGFLLLEQPIRPIASPEGTTLFTVLEDPMFTVK
                     AVALNHRIPSFAYSLEEQFHINVNKQKLREAGLPVGSWLRDVKQYIWQGQPDEFRFTA
                     TLYEEHRREDREFVLGEVKEQFLTISRGQKIAYVVDARYDEDNQAKIVALAREADTLY
                     CESPYLDGDAEKARDRYHLTARQAGIMARKAQVRDLVVFHFSPRYTGQGEVLEREAME
                     AFQGL"
     gene            complement(9212..9928)
                     /locus_tag="VLD60_11190"
     CDS             complement(9212..9928)
                     /locus_tag="VLD60_11190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB45570.1"
                     /translation="MTPPEPGPPSTTTQEEERGAALALLPIAATLDYYALPDWIQTQT
                     PILFAPQVVAYLAFALWAVHNGTILSRFGLKLQQLSAGWKIGLLTGLGLGCFNTAVIL
                     QFVPYLYYDISFLRYTPHALLPVFVMVPWFIFVIALFVELNFRGFLLGRLVTLEAGLW
                     KFPSIQRLSPLALITSALVFAFDPFMVNTFQHLHWIAVWDGLIWGFIWIRTRNLYATI
                     VAHAIEVIVMYCAVRSALME"
     gene            complement(9934..11496)
                     /locus_tag="VLD60_11195"
     CDS             complement(9934..11496)
                     /locus_tag="VLD60_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB45571.1"
                     /translation="MNHRTNRGRWRGLFLTIGTGLILAASPVQATDEAKGQPAERRED
                     ISLADAAIRALKNNLDISISRQTKESRLTDIVVEQAKFDPTLSVNGQYNRQVNPLNRP
                     VFGGTTGTLNTIETFDQRTTSVTVDAQTNLITGGNVDLNYSPSRTNVNQSVATGFLFN
                     PAYTGGLALTLTQPLLRNAGIEVTKTFIRIAQNNAMVEEHVFRDRVLTVIATVEQTYW
                     EVVFANDNLKVAEAALKAAQELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAE
                     KAIRDQEDQLRRLLNPGEEELRLDMRLTPKDQPVVALDPISLQEAIDVAIENRPEIVQ
                     AKKNVDSSDINTKFAKNQLLPTLSFQGTMGLAGLGSDYGNSVNKNFSGDFYNYGAGLV
                     LSYPLGNRSAWSTYSRRQIEGKNAEASLASVRQQIIIGVKEAVRRVQTDFKRIETTRS
                     ARIMAEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAIVDYNKSLSNLARHK
                     ATTLDRYNFQLQ"
     gene            11646..11975
                     /locus_tag="VLD60_11200"
     CDS             11646..11975
                     /locus_tag="VLD60_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45572.1"
                     /translation="MGQATSSVRSFDGGVAPLFNLPGPGNLYLDNQGTQGFLYNPGNN
                     FESYNFRNPTTGQAWSGATMTLGPQLSIGLIQGANQVGSPLVLPGPPRQTPPLLDIQS
                     TILDDIP"
     gene            complement(12017..13546)
                     /locus_tag="VLD60_11205"
     CDS             complement(12017..13546)
                     /locus_tag="VLD60_11205"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB45573.1"
                     /translation="MKSVSACLFGISMLACLMFSAFAAPPLDSSFSTGNWLATALEQI
                     SAERMLADITTLSGPTFRGRQTGSPQDEESAAFVANRFSELRLHRSLASPPQSQTNPL
                     PQREWKQTVPVPTRTIDGDLLLDLVFPHNRSPLQGGFDFLPVLDSPSVDVRAPIVFVG
                     YGLSDPSQGLDDYAGLDVRNKIVLFLRGKPDKYTGPAVHADKERIARQKGAAAYLTAT
                     GPILNAYEQRRGITGKPSAFYSATEPAQQLPGAWISTETAEGILHEGTAGKLTLRALQ
                     EDINRTGTSRSTNTEVQAQMKWASHLAGGTLFNVISVIRGSTPSSDEAVLIGAHRDHF
                     GHQAGLLFGGADDNASGTAVLLEVARILALAPAAPKRSILFLSFSGEEQGLLGSRLYV
                     SQPVVPLQKTIGMINVDHAGIGNGRLTVGVTGFEKSRAQEIGQMAGLSEKLDLFGFFP
                     GGDHVPFKEAGVPTITVVSGGIHPHFHQPTDTANTINPDVLLSVARYVLAFTWELANS
                     P"
     gene            13662..15716
                     /locus_tag="VLD60_11210"
     CDS             13662..15716
                     /locus_tag="VLD60_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB45574.1"
                     /translation="MDQQPNRPHNRLIDESSPYLRQHAENPVDWYPWGEEALSKAKAE
                     DKPILLSIGYSACHWCHVMAHESFESVEIAKVMNQHFINIKVDREERPDLDEIYQKSA
                     QVFNGRGGGWPLTMFLTPDQAPFYGGTYFPPVPRFNLPGFPEVLLGVAEAYRNHRDEV
                     TKNVERVMNALERVGRPKSTTAPLTDQLLDRAADALGRLFDPVYGGFGDGPKFPTVPP
                     LSLMLRQAARRKDQSLQDQVLLQLRRMAAGGIYDHLGGGFHRYSVDGEWKVPHFEKML
                     YDNAQLVRIYLDGWRLTKDEGFRHVVEETLEYVRREMTHPDGAFFAAQDADSEGHEGA
                     FFVWTPEEITAVLGAELGGEFCRFYGVTEGGNFEGKNVLHQLNGFELSAEEQEVADSK
                     LRPARAKLLAAREQRVKPLRDENILTSWNAMMVSAFLDAAVTLGVPAYRAAGEQALTY
                     LLDYAVVNGRVCRTVVGCKGRLNGYLDDAAWLAMALLDAFEATSHRWYLDQARAVTES
                     LLTHFGDKTAGGCFFTSHDHERLIQRMKSGTDSAVPSGNAVAASALLRLFSFTGERRY
                     YENADQILKVFQSVMEGNPYSASAMLCSVDWFLSGPKEIVVVGARGNALTEAMVATVY
                     QRYVPNRALLTVDDPRPVGEVELPLTLGKTSVNGRPTAYVCQHQTCSQPVTEPRQLEA
                     ML"
     gene            15727..15978
                     /locus_tag="VLD60_11215"
     CDS             15727..15978
                     /locus_tag="VLD60_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45575.1"
                     /translation="MAAGDSSKGIYDHLYRRFFENRERYQGYDFQHAPAGSTPPPVVT
                     FREKLRWWTLDRWRRRKQILRERDRLQRDILEIKKRDSE"
     gene            15987..16787
                     /locus_tag="VLD60_11220"
     CDS             15987..16787
                     /locus_tag="VLD60_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45576.1"
                     /translation="MKVDLTTTTNRFPGLFGLVVIAAVLLGEWGYFAVEAPAIQKENQ
                     AEKEANLKGQALGGLFNRWTFDGQKPNEVPADFSILSLGDGAEGAWTIQADVDAPSAP
                     NSLRSESSCPSPSCYRLLVANKLQYEYPDVSVRLRFPAEGGAGRGGIVVGLRDAKNFY
                     AAIVDLASKKLEVIRVIDGQVTQLGQTVIVPKAVDWHSLRVQRDTIISKDVIEAFFDG
                     LLVLSVQDQSLGLGQIGLLVHGNSSLHFDSLHAIPLFSQRPFSAPAAY"
     gene            17053..17355
                     /locus_tag="VLD60_11225"
     CDS             17053..17355
                     /locus_tag="VLD60_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB45577.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAAHGAVASARIITDKFTGQSR
                     GFGFVEMSSDSEAQAAITALNGTQLGGRTLTVNEARPQEPRSGGGGGRGGFGGGRR"
     gene            17504..>18736
                     /locus_tag="VLD60_11230"
     CDS             17504..>18736
                     /locus_tag="VLD60_11230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB45578.1"
                     /translation="MSTFTEMSLPPFLAQRLLQAGFTAPTPIQQAAIQPALEGRDIMA
                     QAKTGSGKTLAFLLPMIERALRPAVLSEGSSFPTSGSPSRKNPRFLILAPTRELALQI
                     EMELRKYAPPSVSSLAVYGGTPIERHHRALRQPPMVVVGTPGRLLDLAGSGHLHLNAV
                     TWLVMDEADQMLDRGFLRDIQRILNLLPKERQTLLFSATFSQEIQQLAHSMQRNPARI
                     SVDPHISSPTTITHAYYVVPAEQSRTQLVHTLLQSVKAGERSMVFCDQKYKVRRLAAK
                     LGGESASVGAITGNHSQAQRERTLGAFRAGRIRSLVATDVAARGLDIPDVAQVIHYEL
                     PTNQNSYVHRAGRTGRAEKEGATFLILSHTEEREYLRMVRQLRIQTKKLPLPAFATMP
                     PVAVAPADDQQARRPRPPR"
BASE COUNT         3992 a         5002 c         5617 g         4125 t
ORIGIN      
        1 gtcgggtggc ttgcgtgcgg cgggccggcg cgataggata aacattatga aagtcccgct
       61 tagtaaagga atttttctca ttgcagcacc caatctgcgc gatccgaatt tccgacaaac
      121 ggtcgtcctc ctgtgtgaac atggggccga aggtgcgctg ggagtggtgg tgaatcgtcc
      181 gaccgcgatg tcaatttcgg aagccctgcc tcaagtgccg gtcctggaag gccagcgcca
      241 tatgctgttt tccggtgggc ctgtccaacc gaatcaggtg atgttgctgt accgtttgaa
      301 tcagttgcca gagaattctc accatgtgtt cgatggagtc tgcctcggcg gcgacatgga
      361 actcgtcgat aagattctca ccacggatca gggacgagag gcgtttcgtg cctatgtcgg
      421 ctattcggga tggggacccg ggcagctcga atcagaaatg aaaacaggat cctggattac
      481 gttgcccgct gatccgagct tcgtgtttga aaaagatccg acccgagtat ggtccgaaat
      541 tctcagcaca ttgggcgaag aatatcgcct ctatgcagac atgccgttcg atccctcttt
      601 gaactaatcg ctgtctcccc cgcgttgcag ccctcgtccc cagaatgtta ggctgcagcg
      661 atgccgacgc agccaacggc tcattcctcc cagcgccccg ctctcatcct catcatcttt
      721 ggtcttaccg gcgcgctttc cgcctgcggg gtggcctaca cgattgtgaa gtcggagtcc
      781 aagctcgaca agctggcggt cccgatgacc aaagcgcaag tcgtcgatga aatcggccgg
      841 cctgatcggg tcctgcgcga cgacggccgc ctccttgtct gggagtattc cctcacggcg
      901 cgcaggcagt ggctctacga actggggctc tgtcctgtgt ccatctggat cggcggctgc
      961 attatctatc cctttacgaa cgtggcaatg gaacatcagc gtgagtaccc cttccacgtg
     1021 gtgctggtga acgaggaact ctgcgcctgg ggtactccgg ctgcaatcct tcaacgccgg
     1081 cgagcctgcg ctgccaccgg tgtgccgatt ggattatcgg gaggggcgcg gggaaggcct
     1141 gagccaatcg tttcgagtaa cgggccgatc aatcgcgaca acatcggccg ctatcggacg
     1201 atggcggtga tgttatttga agatgcgccc ggctcctccg gatccggctc acgcgtggcg
     1261 ggcatcgtta ccaccctcct gctcgatctc gacatccaca tggttgaacg ggcgaaattg
     1321 gatgaggtac tgaaagagca ggtgatccag ctcacgcatg ccgatgacgc caatgtgctg
     1381 aaggtcggga gattggtcgg ggcgcaggcg atcattgtcg gggaagtcca gcaatgggaa
     1441 cgccacgaac aggaacggat gcacagtgtc tcgctgtcgc tgcgcatcat cgatgtcgaa
     1501 accggtctct tgctgtttag cggggaaggg catttagctg attcgacgac cgatgatccg
     1561 gaaagctccg cccgcctcat cgcgcatcgc atactggcgc ggtttggatc tcagacggga
     1621 ttgctcggat ccgggcgcat cggggtgaac tgggagttgc tggaagaaaa gggcggatac
     1681 ttttatctgg tgcgtgaatt gcggagcggg ctgccggctg agaagggggg actcaaggtc
     1741 ggcgaccgca tcatggcctg caacggggcc tcgctgtcag ccgtgaaggc tgaacgtgag
     1801 gccaaacgcc tctgccaggt agaggcggga cagacgctgc tgcttgatat acaacgtgcc
     1861 ggttgtccga tgcagatcga gcttacggcc gataagcggc cagggttgta gggatgaacc
     1921 gccggccata gaggtaaaga ggaatcgcat ggatcgatca gaaacgcctc aggccggatc
     1981 cgatacgttg ataaaaaagc tgcacgagcg gccgcagata ccgctcgccg attggctccg
     2041 ggctccggcg cacgtccatt acatggcgtt ccgaatggcg gacccgccgg agcaacggaa
     2101 agcgagtcgt gaagaattcc ggtcgctcct gtccgccttc gcgatcgagc cggctaagac
     2161 ggtgttgcga gagacttttg gctatggggt caaagaggcg ggcacgggcg atcggttgat
     2221 cgtcgtctgg caggcccata ccgaatacta caattatcag atttggcatt tgcccggccc
     2281 ctcgaatccg gccctgtcgt ttggatcaat atcgtttccg gactacaagt tccccgtcac
     2341 gccgttaggg acggaagtct gtcgcctcga catcctgctc accgcggaat cgttgccgtc
     2401 tcgcgagcgg atgaggacgt tgtttcctgg cccgatgctc tatgggagca agattttcga
     2461 tgaccagaca agtctcgtca ccagttttac gccggacgag caggggcggg agcgctattg
     2521 ggtcggtctc ggtgcgcctc aagcctctgc atcgcggctg aaggatatcg tcgatgcgat
     2581 catgcggatt gaaacctact atcatctgct cctgatgcag aagccgctgt tttcggccgc
     2641 catcgaccaa gtctataaat ttgagcaggt gcatctgaag caacgtgaaa tcatttctgg
     2701 tcacattgcc catgccgact cgcaggcttt gcaacgctgg ctgaatagcc tcacgcagga
     2761 cctgctcaag acgagtcgca ttgccggcaa gctgcacttt gagctgtccg cttcgctgcc
     2821 ctacgacaag atcgtccatg ccacgctggc ctctctagca gagcggccgt tggagtcgta
     2881 ccggccggtg tccgactatg tgctgagcgg cattacggga gtagcggatg gctatcagca
     2941 attactgaaa cgaatcgaga ccttacgaag cggatttgaa gggattattt cgattatcag
     3001 gacccgcgtt gatctgattg tggaaggcca gaacctgact ctgctgcaaa gcgtggacaa
     3061 gacgacgagg agccaagtca ttttgcagca tacggtcgag ggactctcgg tcatcgtgat
     3121 cgcttactat ttggcagggc tcgccggtta tgttttcaaa gggttctccg aaatgggctg
     3181 gctgcggaat gctcatatcg cctcggcagt ctttgttccg atcgcaatcg gccttgcctt
     3241 cgtcattacc acggtgagca agaagtatct gcacaagaag tggtcgggtg agcagccacc
     3301 gacagaaggc tcgaaaacgc caccgcctca atagatgttg tccaagctat cagtggttct
     3361 gtggagggaa agaaaggttt ctcagtagag tgtgaggcta tgtgttcgaa aatacttggg
     3421 ccaagaaacg acctgtgtga gaagtcccaa ccttcgccac ctgttccggc cttccttccg
     3481 ctacgatttg cccgcctgca tcgccaccct ccgggccgag gtcgatgatc caatctgccg
     3541 ccttgatcac atcgagatta tgctccacga cgacaagggt gttgccggcg ttgacgagct
     3601 tgtgcagaac ggcgaggagt ttcttcacgt cgtcaaagtg caggccggtg gtcggttcgt
     3661 ctaagatgta gagcatgttc ttcgcagacg aatctttgag ttccgcggcg actttcagcc
     3721 gctgggcttc gcctcccgag agggtcgttg cgggctggcc gaggcgcagg taaccaaggc
     3781 cgatggatga gagcagatgc agtcgctctt gcagtcgcgg ggtgccggtg aagaaggtga
     3841 gggcttcgtc taccgtcatc gtgagcacat ctgagatggt cttcctccgg tagcgaatgg
     3901 ccagaacctc gggcttgaag cgcataccct cgcaaatctc gcatggcgcg tagatatcct
     3961 caaagaagta catttccagt ttttcgacgc cgctgccctc gcagcgctcg cagcggccac
     4021 ctgccgcatt gaacgagaaa tgtccgggcg aaaacccttg cctcagcgcg tcacgctcgg
     4081 atgcaaacaa ctgccgaatt tcatcgaagc atttgaggta ggtgatcggg ttggagcgcg
     4141 gtgtgcgtcc aatcggttgt tgatcgatga ggcgaacacc gttgagatgc tcgatccctt
     4201 tgatggcttt gaatcgtccc atcgggaggg attccacacg gaatgctcgt gcgagcgcgc
     4261 gatacagcgt atcttcaacg agggtgctct tgcctgaccc cgacacgccg gtgacgcaga
     4321 tcaacatacc gagcgggaac cggaccacga gatcttttaa attgtgttcg ccggctccgg
     4381 caatgacgag caccttgccg ttgcctgagc gccgtgacct tggaagcggg atctcttctt
     4441 ccccacgtag gtaacgggcg gtgagtgctc gccggtcttg gaggaattct ttggccgggg
     4501 cggcgcagac gatttctccg cctttttctc cggactgcgg gccgagctcg acgatgaagt
     4561 cggcagattc gatcatgttg cgatcatgtt ccacgacgac cacggtgttg ccaactgctg
     4621 ccaggtcttt gagaatgcca gccagcaaat ccgtgtcccg cgcatgaagc ccgattgtcg
     4681 gttcgtcgag gacgtagagc gtgccgacga gtcttgcccc aagttgattt gcgagcgaca
     4741 cgcgctgcgc ttcccctccc gacagagtct tggtctgccg ggcgagtgtg aggtatccga
     4801 gaccgacgcg caggaggaag ctgagcttgg cctgtagttg gcggaggata tcggacgcga
     4861 tgtctcgctc gaacggctgg agactcagtt ggtccaacca agacgccagc tgctcgacgg
     4921 taagttccgt gatctgatga atgtcgtggc cggcgatttt cacgaaacgg gcatctggct
     4981 tcagacggct tccgttgcaa atgggacagt taaagggggt acgatagcga ctgagcaaca
     5041 cccgcacgtg cagcttgtaa cgcttaccct ccatgtactc gaagaaatcg ttgatcccat
     5101 cgaacgactt gtcgccctcc cagagcagtt gctgcgtcgg cttgggcagg ttcttgaacg
     5161 ggaccgtgat atccacatcg cgacgcttca ttgcgaggag catctgtttc tgccaccagt
     5221 cggtacccgg tttgctccag ggttcgatcg cgccttgggc cagcgactta ttgtgatctg
     5281 gtataaccaa ttcaggatcg tagcggagca cgttgccgaa acccttgcac tcgggacaag
     5341 cgccgagtgg atgattgaag gagaagagta tgggccttag tggctcgaac gttcggctgc
     5401 aacgttgaca aagaaagctc gtgctgtagg attcaagtcc gtgaccgatg acgtccaccg
     5461 cacagcggcc ttctccttcc aaaaacgcgg tttcgatggc ttcaaccaga cgcgaccgat
     5521 tatcttctcg gacgaccacg cggtcgagca cgacatggag tgcttcggat ttgtggaaag
     5581 aaggcagcat ggcgttatgc aggtcgagga tctcgtcgcc cgccttcaat ctggtgaacc
     5641 ctctggtgag cagcgaccga acgaaggtgt gctccagcgt gggtgcaggg gctgcaactg
     5701 cgaagagtac catcgcgcga gcatcgggaa agcgaatcag cagatcgtcc gctaccgagt
     5761 caggatgaaa cgagcgggcc ttctgatggc agtctggaca gatgggggtg ccgatcttgg
     5821 cgaaaagcag gcgcagaagg tcggcgattt ctgtcgtcgt gccgacggtc gatcgagcgg
     5881 ttcgtacctg gtttttctgt tcaatggcga tagcggggcg aacgttgagc aggcgatcga
     5941 cgtcaggacg agccactttc tcgaggaaca tcctggcata agtcgagagc gattccacat
     6001 agcgccattg accttcggca aagatggtgt cgaaggccag cgacgacttg cctgagcccg
     6061 acaggccggt gatggctgtc actttgttgt gcggaatttt cagcgagatg tttttgaggt
     6121 tgtgctgacg cgcaccctca ataatgaggt cgttggaagc aggtgtcggc gccacaggtt
     6181 ggggcacttg cgatgtcctc tcgcagaaag accgattatc ctagcaagcg gaggggaggt
     6241 cttcaatgag gagaattgca caactgcccg ggaaagacat tggataggtc ggatttgtgg
     6301 attgtctttg ggatatcgtg ttgggatgag agtatgatgg tgtcacggtc gggactggta
     6361 gttcgacaag tccagcggtt cgtgccagga tacaaactct ggaattactg ctcaacaaga
     6421 ggaacgactc tcctatgacg cagtcatccg tgccgaccga ttatttgggg attgggcgtc
     6481 agcttgccgt gttgctgggc tcaagcttgg agggaccctc gggtgaagcg ctgaaagaat
     6541 tggcgcgcgc ctatgatccg tccgcgaacg aatcgcgcat cagcgcagag gtgtttctct
     6601 ttcacaagta tctcatcgtg caaacctgcg tcgggatgtt tccagaggcg caggtggaac
     6661 gcgtcatcgg aggtctattc gcggctttga acgaacgggc gacaggattg gaattcagcc
     6721 aggaacgtca gaacgccatg gagcagatgt ggcagatgcg ggccaagcaa ttcgaccgtc
     6781 cctttacgca agatcgcgag cattttctcg atgaaagtag cgacggcatt tattggaaga
     6841 gatcgatcag ccggttctgt cagaatgtgc gcgagatcga gaaccctccc gatatttggg
     6901 ccggaggcaa aggcccctcg cacaaagcca gcactaccgt cacggaaacg atcgatcacc
     6961 tcgtctcggc gatgagcgag atgagccgcc tgcatttcgg ttcccccgcc taatcgccca
     7021 gaagcgttga atccagcgtt cgcgcagact gtatgcgagg aagtcttaga aagcgccccg
     7081 gggtccagac tcatccagct taactgtagg aaaaatgtga gagcgcatac tccggtgtgc
     7141 ttttctgggc cggatggttg atggataagt aatcgctgaa tttcgaagcc gcctccggag
     7201 tgatgcgggt gacttcgacc ccgaattcta atccgtcggt ccagcgaacg accgcctcat
     7261 cgaccataat tggttgggcc aggtcaggaa agtagatgta gaccgctagt gctgtccctt
     7321 tggctactgg ctgtgttccc ctcgcccgcc aaccctgccg tgacaaatcg agcaatatac
     7381 cttctgcgcg attatggccg gcgagatatt ccatacggca gttgacctgg tgccgtttcc
     7441 catagcgcgg ctcatgccaa gcccctgcgg aaggccgacc ggatgctaca tcatgattac
     7501 ttgatcgcgt agggacgcga gagttgtctc ctcgtttttg tcgcaaaaga ttcaagccat
     7561 gggaaacccc ggcagccgac agtgtggcca ttccagtaat cgtgacaaac tcgagaaggc
     7621 tgatcatgac cgatctcctt gctcagatgc ggtttctgat ctatccccca gatcaatcta
     7681 ttccctgcat ggaattactc tatctctaga aacaaggatc cggtaggtcc tcgaagaggg
     7741 tacctccaat ggagggaagg cctcgaaact aggccacagg agcgtggatt ctcatttacg
     7801 ggcaaaagag gtgagcccta cgggaggggg gcatcagcgg atggccagtt tcacgagccc
     7861 gacacacccc atatataacg tcaaggagat ggccatggtt ggccagaacc ccaggcgcgg
     7921 aacaccgacg atcatggccg tgacgtaaac gatccagggc gggacaaacc acaagagatt
     7981 ccttgcatag cccaccagcg aatcgctgcc gccgttcaag tagagcagaa caaacgtcgc
     8041 gccggtgatg gccggaaatg tgctggcgaa ggcggccaag aacgagcggc cctgcgatcc
     8101 caggtaggtc gaaaggctga caattgctcc tcccagcagg aaatagacgg tgtactttac
     8161 gacgtcgtgc atgcagtgtc tttctcagag cccctgaaac gcttccatcg cctctcgttc
     8221 taagacttcg ccctgtcccg tatatcgcgg agagaaatga aatacaacga gatctctgac
     8281 ttgcgcctta cgtgccatga ttccagcctg tctggccgtg aggtgatagc ggtcgcgtgc
     8341 tttctccgca tcgccgtcca ggtagggtga ttcgcagtag agcgtgtcgg cttcccgggc
     8401 gagagccacg attttcgctt ggttgtcctc atcgtatctg gcatcgacaa cgtaggcgat
     8461 cttctgccct cgcgagatcg tgaggaactg ttccttcact tcacccaaga cgaattcccg
     8521 gtcctcacgg cgatgctcct catacagagt ggcggtgaag cggaactcat ccggttgacc
     8581 ctgccagatg tactgtttca cgtctctgag ccaggagccg accggcaggc cggcttcccg
     8641 tagcttttgc ttgttgacgt tgatatggaa ctgttcctcc agggaataag cgaacgaggg
     8701 aatccgatgg ttcaaggcaa cggctttcac cgtaaacatc gggtcttcca gaacggtgaa
     8761 gagcgtcgtt ccttccggtg aagctatagg gcgaatgggt tgttctaaaa ggagaaatcc
     8821 attggtggcg tgaaagaaag cctgtctgac ctgctgcccg tggaattcct gaacctcgat
     8881 cgaaagcgga tagccatcga ccaaattcca cgtatagcct cgcagctttc cctgtacgtt
     8941 gtcgatcaat ccaggagggc cgtacagccg gagcttcttg ccacgcccta aagcgacgcg
     9001 aacgactgca tcgaatccga tgaagtgatc catgtgcgta tgggagatga aaatatcgtt
     9061 tgcgcgcaga agcctcgttg gtcccagtga atcattatgc cccgcgtcga acagaagagc
     9121 ccgccttgac cagcggattt ccacgtagac ccctgggtca ccgaagacat cgtttacgag
     9181 gtaactggag aaggatggat tcataacctg ctcattccat cagagcggac cgtacggcac
     9241 aatacatgac gatgacttca atggcatggg ccacgatcgt tgcgtagaga ttgcgtgtcc
     9301 tgatccagat gaatccccag atcaacccgt cccagaccgc gatccagtgc aggtgctgga
     9361 aagtattcac catgaacgga tcgaatgcaa acacgagcgc gctggtgatg agtgcgagcg
     9421 gggaaagacg ttggatcgat gggaatttcc aaaggcctgc ttcaagcgtg accaatcgcc
     9481 ccaagaggaa gccacggaaa ttcaactcga caaagagggc aatgacaaag ataaaccagg
     9541 gcaccatcac aaaaacaggt agcagtgcat gaggggtata cctcaaaaat gagatgtcat
     9601 aataaagata tggaacgaac tggaggatca ccgctgtgtt gaagcagccc agaccaagcc
     9661 ccgtcagtag tccaatcttc caaccggcgg aaagttgctg cagcttgagg ccgaaccgag
     9721 aaaggatcgt accgttgtga acggcccaga gggcaaaggc gagataggcg acgacttggg
     9781 gagcgaacag gatcggcgtc tgcgtttgga tccaatcggg aagagcgtag taatcgagtg
     9841 tggctgcgat aggaagcagc gccagcgctg ctccccgttc ctcttcctga gtcgtggttg
     9901 aaggaggacc aggctcaggc ggcgtcatgg ccgttactgt agctggaaat tgtagcgatc
     9961 cagcgtcgtc gccttgtgtc gagcaagatt cgacagcgac ttgttgtagt ccacgattgc
    10021 acgcagctcg ttgccttgtg cggtagcaag atcccgttgg aaatcaagca cgaagcgcgt
    10081 cgtgctcagt ccaactttca gccgttcctg ttcggcttgg agttgctttt ccgccatgat
    10141 gcgggccgac ctcgtcgttt cgatacgttt gaaatcagtc tgcaccctcc ggactgcttc
    10201 tttgactcca atgatgatct gttggcggac gctggccaac gacgcttctg catttttgcc
    10261 ctcgatctgt cggcggctat aggtactcca ggccgagcgg ttgccgagag ggtagctgag
    10321 tacaaggccg gccccataat tgtagaaatc accgctgaag ttcttgttca cagaattgcc
    10381 gtagtcactg ccgagccccg ccaagcccat cgtgccttgg aaggacagcg tggggagcaa
    10441 ctggtttttg gcgaatttgg tgttgatatc actggaatcg acgtttttct ttgcctgaac
    10501 gatttccggc cggttctcga tagcgacatc aatggcttct tgaaggctga tcggatccaa
    10561 cgccacgacg ggctgatctt tcggtgtcag ccgcatgtcg aggcgaagtt cctcttctcc
    10621 gggattcagt aatcggcgca attgatcttc ctggtcgcga atggctttct ccgccacgag
    10681 gatttgttca acgcgtgacg ccacagcagc ttcggcttgg agcacatcga caattgacat
    10741 gacaccggct ttggccttgg ctcgattact ggccagcagt tcctgcgccg ctttcaacgc
    10801 cgcttcggcg actttcagat tatcgttggc gaagacgact tcccaatagg tctgttccac
    10861 ggtggcaatg acagtcagga cgcggtcgcg aaacacatgt tcttccacca tggcgttgtt
    10921 ctgggcgatc cggatgaacg tcttcgtcac ctcgatgccg gcattccgga gcaacggttg
    10981 ggtgagtgtt aaggcgaggc cgcctgtgta ggccgggttg aacaagaatc ctgtagccac
    11041 gctctggttc acgtttgtgc ggctagggct gtagttcaag tcgacgttac cgccggtgat
    11101 cagattcgtc tgggcgtcta ccgtgacgga ggtggtacgt tggtcgaatg tctcgatggt
    11161 gttcaatgtt ccggtggttc caccgaagac cggccggttc aagggattca cttgacgatt
    11221 gtactgtccg ttgacgctga gggtcgggtc aaacttcgcc tgctccacga cgatatcagt
    11281 cagccggctt tcttttgtct ggcggctaat gctgatgtcg aggttgtttt tgagtgcacg
    11341 gatggcagca tcggcgagtg agatatcctc gcgtcgctcc gccggctgac ctttggcctc
    11401 gtctgttgcc tgaacgggac tcgcggccag gattagtccg gttccaatgg tgaggaagag
    11461 cccacgccag cggcccctgt tggttcggtg gttcataaag ccccccttga tcgacaagga
    11521 caagcccccc atactataat gaatttatga aagcctgtca tggacttcct cagaagggac
    11581 cgtgccgtct cctccttgca gtaattgttt tgacaggagc gtccgcgtgg tgcgaatcac
    11641 cggcgatggg gcaagccacc tccagtgtcc gttcgtttga tgggggggtc gctcctcttt
    11701 ttaatctacc ggggccggga aatctctacc tggataacca ggggacacaa ggctttctct
    11761 acaaccccgg aaataatttt gaatcgtata acttccgtaa tccgacgacc ggtcaggcct
    11821 ggagcggtgc gaccatgacg ttagggccgc agctatccat tgggttgatt caaggcgcca
    11881 accaggtcgg ctcgccactg gttcttcctg gtcctccccg gcaaacccct ccgctcctcg
    11941 atattcaatc cacgatcctg gacgatatcc cctaacagga ctattccttg tacccagccg
    12001 caccctatgc gcatgcttag ggagagtttg cgagctccca ggtgaaagcc agaacgtatc
    12061 gggccacgga cagtagaaca tccggattga ttgtgttagc ggtatcggtc ggttgatgga
    12121 agtggggatg gattccgccg ctgacgaccg tgatcgtggg cacaccggct tccttaaatg
    12181 gaacatgatc gccaccgggg aagaaaccga acagatcaag cttttccgat aggcctgcca
    12241 tttgcccgat ttcctgtgcc cggctctttt caaatcccgt cacgccaact gtgagccgtc
    12301 cgtttccaat ccctgcatgg tccacattga tcatgccaat cgttttttga agtggaacga
    12361 ccggttggga gacgtacagc cgcgagccca agagaccctg ttcttcgccg ctgaacgaga
    12421 ggaatagtat cgatcgcttc ggtgcggccg gtgccagagc gagtatccgg gccacttcaa
    12481 gcagcacggc cgtgccggag gcgttatcat ctgcgccccc aaacaacaag ccggcttgat
    12541 ggccgaagtg atctcgatgg gccccgatca gcacggcttc gtcgctgctg ggtgtagagc
    12601 ctcgtatgac agagatgaca ttgaagagcg tgccgccggc aaggtgactc gcccacttca
    12661 tctgagcctg tacctctgta ttcgtcgatc gtgacgtgcc ggtgcggttg atgtcttcct
    12721 gtaaggcgcg aagggtgagc ttgcccgcgg ttccttcgtg gaggattcct tcagcagtct
    12781 ccgtgctgat ccaggctccc ggcaattgct gcgccggctc tgtggcggaa tagaacgcgc
    12841 tgggtttccc ggtgatgcca cgacgttgtt cataggcatt caagattggg ccggtcgcgg
    12901 tcagataggc cgcggcacct ttctgtcgcg cgatacgctc cttgtcggca tggacagcag
    12961 gacctgtgta tttgtccggt ttccctcgaa ggaagaggac gatcttgttg cgcacatcga
    13021 ggccggcata gtcgtcgagg ccctgcgagg gatcggagag cccatagccg acgaagacga
    13081 taggcgcgcg gacatcgacg gaaggtgagt cgagcaccgg cagaaagtcg aaccctcctt
    13141 ggagcggaga acggttgtgg ggaaaaacca gatccaacag aagatcgcca tcgatggttc
    13201 gggtgggaac agggacggtc tgtttccatt cccgttgggg gagcggattg gtttggctct
    13261 gcggaggaga ggcgagtgaa cgatggagtc ggagttcgct gaaacgattc gcaacgaagg
    13321 cggcggattc ctcatcctgt gggctgcccg tttgccggcc tcgaaatgtc ggcccgctta
    13381 atgttgtgat gtccgccagc atccgttcgg cagagatttg ttccagagcc gtggcgagcc
    13441 aattgccggt cgaaaaggag gaatcaagtg gaggggccgc aaaggcagaa aacatcaaac
    13501 aggccagcat cgaaatcccg aacaggcatg cgctgacaga tttcatgagc cctcgaatga
    13561 gactgtgcgg cggtgcgcca ctataacata gagcgtgaga ttcattgcag ggtgccgggg
    13621 tgcagagtac tatgacgagc ggtcgaacga atggagcatc cgtggatcaa caacccaacc
    13681 gtcctcacaa tcgcctcatc gacgagtcga gcccctatct caggcagcat gccgagaatc
    13741 cggtggactg gtatccctgg ggcgaggagg cgctctctaa agctaaggcg gaagataagc
    13801 cgattctgct gtcgatcggc tattcagcct gccattggtg ccatgtaatg gcgcacgagt
    13861 catttgaaag cgtcgagatc gcgaaggtga tgaaccagca tttcatcaac atcaaagtgg
    13921 atcgggaaga gcggccggat ctggacgaga tctaccagaa gtcggcgcaa gtctttaacg
    13981 ggcgaggggg cggctggcct ctgaccatgt tccttactcc cgatcaagct ccgttttacg
    14041 gtggaaccta ttttcctccg gtaccgcgat tcaatctccc gggttttccg gaggtcttat
    14101 taggtgtcgc cgaagcctat cggaaccacc gggacgaggt cacaaagaat gtcgagcggg
    14161 tcatgaatgc gctggaaaga gtcggccggc cgaaatcaac gactgccccc ctcacggatc
    14221 agctcttgga tcgtgcggca gacgcgctag gtcggctatt cgaccccgtt tacggaggat
    14281 tcggcgatgg cccaaaattt ccaacggtac caccgctcag tctgatgttg cggcaggcgg
    14341 cacggaggaa agaccaatct ttacaggatc aggtgctgct ccaactccgc aggatggcgg
    14401 cgggcgggat ctacgatcac cttggcggag gattccatcg ctattccgtc gatggcgaat
    14461 ggaaagtccc ccatttcgaa aagatgctct acgacaatgc gcagctcgtg cggatctatc
    14521 tcgatgggtg gcggttgacc aaagatgagg ggttccgtca tgtcgtcgaa gagactctgg
    14581 agtatgtgcg gcgcgagatg acgcatcccg acggcgcatt ttttgccgcg caggacgcag
    14641 acagcgaagg gcacgaaggg gcgttcttcg tctggacacc agaagaaata acagcggtcc
    14701 tgggggccga actggggggt gagttttgtc ggttttacgg cgtgacggag ggcgggaatt
    14761 ttgaaggtaa gaacgtcttg caccagctca acggatttga gttatcggcc gaagagcagg
    14821 aggtggccga ttcgaagctg cgtcccgccc gagcgaagct gctggcagca cgggagcagc
    14881 gggtgaagcc actccgtgac gaaaatattc tgacaagttg gaatgcgatg atggtatcgg
    14941 ccttcttgga tgccgcggtg acacttgggg tgccggccta tcgtgctgct ggggaacagg
    15001 cgctgacgta tctcttggat tatgccgtcg tgaatggccg ggtttgtcgc acggtagttg
    15061 gatgcaaggg acggctcaac ggatatctgg acgatgcggc ctggttggcc atggcgttac
    15121 ttgatgcgtt cgaagccaca tctcatcggt ggtacttaga tcaggcccgt gcggttacgg
    15181 agagcctgct gacgcatttt ggggacaaga cagcaggtgg atgtttcttt accagccatg
    15241 accatgaacg actgatccaa cggatgaaat ccgggacgga cagcgccgtc ccatcgggaa
    15301 atgcagtggc tgcttcggcc ctcttgaggc tcttctcttt caccggtgag aggcggtatt
    15361 atgaaaacgc cgatcagatt ctgaaggtgt tccagtcggt aatggaaggg aatccttata
    15421 gtgcctccgc catgctctgt tcggttgatt ggttcctatc cggcccgaaa gaaatagtgg
    15481 tcgtgggagc cagaggcaat gctttgactg aagcgatggt ggcgactgtg tatcaacggt
    15541 atgtccccaa tcgagcattg ttgacggtag atgatcctag accggttggc gaggtggagt
    15601 tgccattgac gctaggaaag acgagcgtca acggaaggcc gaccgcgtat gtgtgtcagc
    15661 accagacatg ttcgcaaccg gtgacggaac ctcggcagct tgaggcaatg ttataggaga
    15721 atgacggtgg cagcaggcga ttcatccaaa ggcatctacg atcatctgta ccggagattc
    15781 ttcgagaatc gagagcggta ccaaggttat gatttccagc atgcaccggc tgggagtacc
    15841 ccgccgccgg tcgtaacatt tcgcgagaag ctgcggtggt ggacattgga tcgctggcgt
    15901 cgcaggaagc agatcttgag ggagcgtgat cggcttcagc gggacattct tgaaatcaag
    15961 aaacgtgatt cagagtaatt tctagaatga aagtcgatct tacaaccacg acgaatcgat
    16021 ttccgggatt gttcggtctg gtcgtcattg ctgccgtgtt attaggggag tgggggtact
    16081 tcgctgtaga ggctccggct attcagaaag agaaccaggc agagaaggag gccaacctga
    16141 aaggccaagc gttggggggc ctattcaatc gttggacatt cgatggtcag aagccgaatg
    16201 aagtgccggc cgatttttca attctgagtc tgggtgacgg ggccgaagga gcgtggacga
    16261 tacaggcaga tgtcgacgca ccctctgccc ccaacagcct gcgctccgag tcgtcatgtc
    16321 catcaccttc ctgctatcga ctgctggtcg ccaacaaatt gcagtacgaa tatccggacg
    16381 tttcggtcag gttgcgattt cccgcagagg gaggtgccgg ccgagggggg atcgtggtcg
    16441 gcctgcgaga tgcgaagaat ttctacgcag ctatcgtcga tttggcttcc aagaagctcg
    16501 aagtaatccg agtgatcgac ggtcaggtca ctcaattggg tcagacggtg attgttccca
    16561 aggcggtcga ttggcattcg ctcagggtcc agcgcgatac catcatcagc aaggacgtaa
    16621 ttgaggcttt ttttgacggg ttgctggtgc tatccgtcca agatcaatcc ctgggactcg
    16681 gtcagatcgg actgctggtt cacggaaatt catccttgca tttcgacagc ttgcacgcaa
    16741 tccccctgtt ttctcagcgc ccattttcag ctcctgcggc atattaatca tgaacttcaa
    16801 ggagcggagt ttcactgctc gcttgctttt gaccagattg tggagtagag tgcagttcgt
    16861 gtatcgccgc gaaaggcctg gtggagatac agtagagagt gacaaggcct gatacgggtg
    16921 ttgttttggt tctgcccggg gatctgatcg gaagccagcc cagagtcgcc atcgtgtttg
    16981 cgtcttcggc ccattccttc ctcacctctt ctcgagtcca agacaacatc attttctcaa
    17041 aggaggaacc caatgggttc gaagatttat gttgggggtt tgccgtattc cacaaccgag
    17101 cagcaactga gcgatctatt tgctgcgcat ggggcggtgg catcagcccg aatcattacc
    17161 gataagttca ctggtcaatc gcgaggcttc ggcttcgtgg aaatgtccag cgactcggag
    17221 gctcaggctg cgattaccgc gttgaacggt acccaactgg gcggccgcac gttgacggtc
    17281 aacgaagcgc gtccgcagga accacgctct ggtggcggcg gaggccgcgg cgggttcggt
    17341 ggcggccgac gctaagcacg agccgttgat tttcacatcg gggctgtcga caaggcagcc
    17401 ccgcttattt ccttcctaga attgcccatc tgcgagatgc gtttcccggt cgcctgatcg
    17461 accgatctgc agtcccagga agttgatcca tacaaggagt gctatgtcta cgtttactga
    17521 gatgagtctg cccccgtttc ttgcccagcg tttgctacag gctgggttta ctgcaccgac
    17581 acccattcaa caggctgcga ttcagcctgc gcttgaaggg cgggatatca tggcgcaagc
    17641 caagacggga agcggaaaaa ctttggcgtt tcttctgccg atgatcgagc gggctttgcg
    17701 gcccgccgtt ttgagcgagg gatcgtcctt tcccacgtcg ggatctccaa gcaggaagaa
    17761 cccacgattc ttgatattgg cgccgacgag agaactcgcg ttgcagatcg agatggagtt
    17821 gcgtaagtac gcgccgccat ccgtttcctc cctggccgtc tacggcggga cgccgatcga
    17881 gcgacaccac cgcgcgcttc gacagccgcc gatggtcgtt gttggaacac ccggtcgtct
    17941 gctcgacctt gccggttccg gccacctgca tttgaacgcc gtgacctggc tggtgatgga
    18001 tgaagctgat caaatgttgg atcgcggatt cctccgggat atccagcgaa ttctgaacct
    18061 gttgccgaag gaacggcaga ctctgttgtt ctccgccacc ttttcacagg agattcagca
    18121 gctcgcccac agtatgcaac gcaatccggc caggatttcg gtcgatccgc atatcagcag
    18181 cccgaccacg attacgcatg cctattacgt cgtaccggcg gaacaatcgc gcacgcaatt
    18241 ggtgcacacg ttgttgcagt cggtaaaggc gggagaacga tcgatggtgt tctgcgacca
    18301 aaaatacaag gtacgccgtc ttgccgcgaa attgggcggc gagtctgcgt cggtgggcgc
    18361 aatcacggga aatcacagcc aagctcaacg ggagcggacc ttgggcgcct ttcgggccgg
    18421 ccggatccgt tcgctcgtgg cgaccgacgt cgccgcacga ggcctggaca ttccggatgt
    18481 cgcgcaggtg attcactacg agttgccgac caatcagaat tcctatgtgc atcgggccgg
    18541 acggaccggt cgagcggaga aggaaggggc gacctttctt atcctctctc acacagagga
    18601 gcgggagtat ctccgcatgg tgcgacagct gaggattcag accaagaagc ttccacttcc
    18661 agcctttgca acgatgcctc cggttgcggt ggctccggcg gatgaccaac aagcgaggcg
    18721 tccgcgaccg cctcgg
//
LOCUS       DATIFR010000197         5476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2627869, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000197 DATIFR010000000
VERSION     DATIFR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627869"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2110
                     /locus_tag="VLD60_11235"
     CDS             <1..2110
                     /locus_tag="VLD60_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSB45579.1"
                     /translation="GDPGKAESFNPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEI
                     IETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDIAQDVIINEL
                     DCGTHDGIIVSALVEGGEIIQPLEERVLGRLAAEDIRDPVTGEIIVSRNEEINEELTK
                     SIVEAGVDRVKIRSVLTCQSLRGVCQACYGRDLARGRLVEKGEPVGVIAAQSIGEPGT
                     QLTMRTFHIGGTASKVVEQTVLEAKHAGRIRFMSFDAKKNADLHNGGIAVRNKEGEWV
                     VMNRNAKIAIVDDSGREREKYPVVYGAKIKIKDGDPVALGQKLVEWDPYSLTILTEVG
                     GKVAYGDIVEGVTMKEEFDEVTGLSRKVIIEHTGQTLRPRVSIKDESGKTAKVPGGSN
                     TVARYLLPVGAHIFVEKGSLVHPGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKE
                     QAVISEIDGEVSYGGFVKGQRKVLVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLM
                     DGSANPHDILDVLGPNELQKYLVDEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDP
                     GDTQFLPGSQVSKSVFEKENERVLEKDGKPALGKPVLLGITKAALTTDSFISAASFQE
                     TTRVLTEAAINGREDNLLGLKENVIVGRLIPAGSGFEEYRDTFVISEKPEVAAVGSAP
                     AAAAVTSDVVPSAAGDASRS"
     gene            2225..2596
                     /gene="rpsL"
                     /locus_tag="VLD60_11240"
     CDS             2225..2596
                     /gene="rpsL"
                     /locus_tag="VLD60_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSB45580.1"
                     /translation="MPTINQLVRKGRNLVKSKTKSPALKSCPQKRGVCLRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHLVRGAL
                     DAVGVADRKQSRSKYGAKRPK"
     gene            2636..3106
                     /gene="rpsG"
                     /locus_tag="VLD60_11245"
     CDS             2636..3106
                     /gene="rpsG"
                     /locus_tag="VLD60_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSB45581.1"
                     /translation="MPRSRFLDQREVLPDVRYRDKLVGKFINTLMSDGKKSTAERICY
                     GAFDVIQEKTGGDPLKVFKAAIDNVKPIVEVKSRRVGGASYQVPVEIRPARRVSLALR
                     WLSEYSRTRGGKSMREKLAAELLDASNNTGASVKKREDVHRMAEANKAFAHYRW"
     gene            3220..5301
                     /gene="fusA"
                     /locus_tag="VLD60_11250"
     CDS             3220..5301
                     /gene="fusA"
                     /locus_tag="VLD60_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSB45582.1"
                     /translation="MARQTSLEHTRNIGIMAHIDAGKTTTTERILFYTGMTHKIGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVQGVEPQSETVWRQADKYNVPRIAFMNKMDRIGADFYASVQSIIDRLGANPV
                     PIQIPIGREGEYRGTVDLVKMKGFFYDDETLGAKYKIDDIPANLLDQAKEYREKMIDK
                     VAEFDDQVMEKYLNGHSLSEEEVRRAIRAGTIAMKITPVLCGSAFKNKGVQQLLDAVI
                     DYLPSPLDIPPVKGIDPNTGKEVERKPDDNDPFSALAFKMMTDPFAGQLVFFRVYSGT
                     LKTGSPVLNVTKGTKDRIGRLLKMHANKREEIDVAYAGDICAAVGLKGATTGDTLCEE
                     KQPVLLEVMKFPEPVIAMAIEPKTKQDQEKMGFALQKLAQEDPSFRVRTDEETTQTII
                     AGMGELHLEIIVDRMLREFKVEANVGKPEVAFRETIRRKAEAESKYVKQTGGRGQYGH
                     VVLTVEPSEPGKGFEFVNKIVGGVIPKEFIPAVEKGVRERLDAGVIAGYPLRDIRVTL
                     TYGSYHDVDSNEMAFKIAGSMAFADACKKADPVLLEPIMKVEVLVPQEFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEASVPLSEMFGYATDLRSRTQGRATYSMEFDRYDQVPRQI
                     AEGIIAKYRGE"
     gene            5339..>5476
                     /locus_tag="VLD60_11255"
     CDS             5339..>5476
                     /locus_tag="VLD60_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB45583.1"
                     /translation="MAKAKFERKKPHVNIGTIGHVDHGKTTLTAALTKICADRGMAKF
                     IS"
BASE COUNT         1251 a         1367 c         1766 g         1092 t
ORIGIN      
        1 cggagatccg ggcaaggcgg aatctttcaa tccgatcttc atgatggccg actccggtgc
       61 gcgcggcagc tcgcagcaga tccggcagtt gggcggcatg cgcggcctga tggccaaacc
      121 atccggcgaa atcatcgaaa ccccgatcac ggccaacttc cgcgaagggt tgacggtgtt
      181 gcagtacttc atttcgaccc acggtgcgcg caaaggtctt gccgacactg cgttgaaaac
      241 ggcgaactcc ggctatctga cccggcggct cgtcgacatt gcgcaggacg tgatcatcaa
      301 cgagctcgat tgcggaacgc acgatggcat catcgtcagc gcgctcgtgg aaggcggaga
      361 aatcattcag ccgctggaag agcgtgtctt ggggcgtttg gcggcagagg acattcgcga
      421 tccggtcacc ggcgaaatca tcgtgtcgag gaatgaggaa atcaatgaag agctgaccaa
      481 gtcgatcgtg gaggccggag tcgatcgggt gaagatccgg tccgtgctga cctgccaatc
      541 actgcgcggc gtctgccaag cgtgttacgg gcgagacctg gcgcgcggcc gtttggttga
      601 aaagggtgag cctgtcggag tcatcgccgc gcagtccatc ggcgaaccag gtacgcagtt
      661 gacgatgcgg accttccaca tcggcggtac ggccagcaag gttgtcgaac agaccgtgct
      721 cgaggctaag catgccggac ggatccggtt catgagtttc gatgccaaga agaacgccga
      781 tctgcacaac ggcgggatcg cggtgcggaa taaagaaggt gaatgggttg tcatgaatcg
      841 caatgcgaag attgcgatcg tcgatgacag cggccgtgaa cgtgaaaagt acccggtcgt
      901 ctacggcgcg aaaatcaaga tcaaggacgg cgacccggtt gccctggggc agaagttggt
      961 cgagtgggat ccgtactcgc tgaccatcct gaccgaggtg ggcgggaagg tggcctacgg
     1021 tgacatcgtg gaaggcgtca ccatgaagga agagtttgac gaggtcaccg gtctctcgcg
     1081 caaagtcatc atcgaacaca ccggccaaac actccgcccg cgcgtgtcga ttaaggacga
     1141 gagcggcaag acagccaagg ttcccggcgg ttccaacacc gttgcgcggt acctgttgcc
     1201 ggtcggagcc cacatcttcg tcgagaaggg ctccttggtg catcccggcg acgtgttggc
     1261 caagattcct cgcgaaacga cgaagaccaa ggacatcacc ggaggtctcc cgcgtgttgc
     1321 agaattgttc gaggcgcgga aaccgaagga gcaggccgtc atcagtgaga tcgacggaga
     1381 agtgtcctac ggcggtttcg tgaagggtca gcgcaaggtt ctcgtcgaca acaagatggg
     1441 cgacgtgaag gaatacttta ttccgaaggg caagcacgtc aacgtgcacg agggtgactg
     1501 ggtgcgggcc ggcgaaccgt tgatggatgg atcggccaat ccccacgaca ttctcgatgt
     1561 gcttggtccc aacgaactgc agaaatacct ggtggatgaa gtgcaggacg tctatcgctt
     1621 gcagggtgtc tcgatcaacg acaaacacat cgagatcatt gtgcggcaaa tgctgcgcaa
     1681 agtccggatc gaagatccgg gcgacacgca gttcctgccc ggcagtcagg tgagcaagag
     1741 cgtgttcgag aaggaaaacg aacgggtgct cgaaaaggat ggaaagcctg cgttgggcaa
     1801 gcccgtcctg ctcggcatca cgaaggcggc cttgacgacc gatagcttta tctcggcggc
     1861 gtccttccag gaaaccacca gagtgctcac cgaagctgcc atcaatggac gcgaagataa
     1921 tttgttgggc ctgaaggaaa acgttattgt gggacgcttg atcccggccg ggtccggatt
     1981 tgaagagtat cgagacacgt ttgtcattag tgaaaagcca gaggtggcag cggtaggatc
     2041 cgctccagca gcggctgcgg tcacttcgga tgtggttcca tcggccgccg gcgacgcgtc
     2101 acggtcgtaa agattagcgt gggaaacgcg cctcacttga cagcgggcag gcgcatcatt
     2161 ataatccgac ggctttgctc ttgagggcag ggcttagtgc gctctaggag aaggttcgaa
     2221 cacgatgccg acaatcaatc agcttgtacg aaaaggccga aacctcgtta agtcaaagac
     2281 gaagagtccg gctctgaaat cctgcccgca gaaacgcgga gtctgtcttc gtgtctacac
     2341 gacgacgccg aagaagccga actcggcctt gcgaaaagtc gcgcgtgtgc gtctgacgaa
     2401 tggtatggag gtcacgactt acattcccgg cgtcgggcac aatctgcagg agcactccat
     2461 cgtccttgtt cgcggcggtc gcgtgaagga ccttcccggt gtccgctacc acttggtgcg
     2521 cggtgccctg gatgcagtgg gagttgccga tcggaagcag agtcgttcga agtacggagc
     2581 caagcgtcca aagtagtgtg gcgtcgccgc acattaaaca ggaagaatag ttcacatgcc
     2641 acgaagtaga tttttagatc agcgcgaagt gcttcccgat gtgcggtatc gcgacaagct
     2701 cgtcgggaag tttatcaaca cgctcatgag tgatgggaag aagagtaccg ctgagcggat
     2761 ttgctacggg gctttcgatg tcattcagga gaaaaccggg ggcgacccgc tgaaagtctt
     2821 taaggcggcg attgataatg tgaagccgat cgttgaggtg aagtctcgtc gcgtgggagg
     2881 ggcctcgtac caggttccgg tcgaaatcag gcccgcgcgc cgggtgtcgt tggcgcttcg
     2941 ttggctgtcg gagtattcgc gcactcgcgg cggaaagagc atgcgggaga agttggccgc
     3001 cgagttgctc gacgcatcga ataatacagg ggcttcggtg aagaagcggg aagacgtgca
     3061 tcggatggcg gaagcgaata aagcgttcgc ccattatcgc tggtaacgtc gttctgtgct
     3121 gcagttgagc cgtgctgcga tccgcagacg cgggtgcccc tgaatcgcag cagtgtaatt
     3181 cggcttgacg tgcagcacgt gtgtttttag aggaatcaag tggctcgtca aacatcacta
     3241 gagcatacga gaaacatcgg catcatggcc cacattgatg ccggaaaaac gacgacgacc
     3301 gagcgtattc tcttttatac ggggatgacg cacaagatcg gggaagtcca tgaaggtgct
     3361 gcgaccatgg actggatgga gcaggagcgc gagcgcggga ttaccattac cgcggcggcg
     3421 acaacctgtt tctggcgcga tcaccgcatc aatattatcg atacgccggg tcacgtcgac
     3481 tttacgatcg aggtcgagcg ctcgcttcgc gtattagatg gtgctgtggc tgcatttgat
     3541 tccgtccagg gtgtggagcc gcagtcggaa acggtgtggc ggcaagcgga taagtacaat
     3601 gtgccgcgca ttgccttcat gaataaaatg gatcgtatcg gggctgattt ttacgccagc
     3661 gttcagtcga tcattgatcg actgggggcg aatccggttc cgatccagat tccgatcggt
     3721 cgggaaggtg aatatcgcgg gaccgtcgat ctggtgaaga tgaagggatt cttctacgac
     3781 gatgagactt tgggcgcgaa gtataagatt gacgatatcc ctgcgaacct cctggatcag
     3841 gccaaggaat atcgcgaaaa gatgatcgat aaggtcgcgg agtttgacga ccaggtgatg
     3901 gaaaagtatt tgaacggtca ctcgctctcc gaggaggagg tcaggcgggc catccgtgcg
     3961 ggaaccatcg ccatgaagat tacgccggtc ctctgcggct cggcttttaa gaacaagggt
     4021 gtgcagcagt tgctggatgc ggtcatcgat tatctgccgt cccctctcga tattccaccg
     4081 gtcaagggca tcgatcccaa cactgggaag gaagttgagc gaaagcctga cgacaacgat
     4141 ccgttttcgg cccttgcctt caagatgatg actgatcctt ttgcgggtca gctggtgttc
     4201 tttcgcgtct actccggcac gctgaagacg ggatctccgg ttctgaacgt gacgaagggc
     4261 acgaaggatc gcatcgggcg gttgcttaag atgcacgcga ataaacggga agaaattgat
     4321 gtggcgtacg ccggagacat ttgtgccgcc gtcggtctga agggcgccac aacaggagat
     4381 acgctgtgtg aggaaaagca gccggtcctg ctggaggtga tgaaattccc cgaaccagtt
     4441 atcgccatgg cgattgagcc gaagacgaag caggaccagg agaagatggg gttcgcgctc
     4501 cagaaactcg cgcaggaaga tccctcgttc agagttcgca cagacgagga aacgacgcag
     4561 acgattattg ccggcatggg cgagttgcac cttgagatta tcgttgatcg catgctccgt
     4621 gagttcaagg tcgaagccaa cgtcggtaag cccgaggtcg ccttccgtga aacgatccgc
     4681 agaaaagccg aagccgagtc gaagtatgtc aagcagacgg gtggtcgcgg tcagtacggc
     4741 catgtcgttc tgacggttga accgtccgag cccggcaagg gttttgagtt cgtcaataag
     4801 atcgtcggcg gagtgattcc gaaggagttt attccggccg tcgagaaggg cgtcagggag
     4861 cgcttggatg caggtgtcat tgccgggtat ccgctgcgcg atattcgcgt gacgcttacg
     4921 tatggttcct atcacgatgt cgactcgaac gaaatggcat tcaagatcgc cgggtcaatg
     4981 gcttttgccg atgcctgtaa gaaggcagat cctgtgttgc ttgagccgat catgaaagtg
     5041 gaagtcctgg ttccccaaga gttcatgggc gatgtgatcg gaaacttgaa tgggcgtcgc
     5101 gggaaagtgc agggcatgaa ggttcgggcc ggtgcgcagg ctatcgaggc gtccgtgccg
     5161 ctcagtgaaa tgtttgggta cgccaccgac cttcggtcgc gcacgcaggg gcgcgcaacg
     5221 tacagcatgg aatttgatcg atatgaccag gtccccaggc agatcgccga agggatcatc
     5281 gccaagtatc gtggggaata acgttagctt cttgttggtg tagagggagg gagttggtat
     5341 ggcgaaggcg aaatttgagc ggaagaagcc gcatgtgaac attgggacga tcgggcacgt
     5401 ggaccacggc aagacgacgt tgacggcggc gttgacgaag atctgcgccg atcggggcat
     5461 ggcgaagttc atcagc
//
LOCUS       DATIFR010000198        21240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_712613, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000198 DATIFR010000000
VERSION     DATIFR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712613"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..658)
                     /locus_tag="VLD60_11260"
     CDS             complement(125..658)
                     /locus_tag="VLD60_11260"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HSB45584.1"
                     /translation="MSTKLKGDIAEQAAILQGLKRGGGILKPIGDRLPYDLVFDIQGT
                     LIKVQVKCAWFDESRGNFVIDNRRTKTNRRVMIREVYTPSDFDFALAYLPAEDLFYVF
                     PVDAFISYASEIHLVETEKRQRKPRSAAYRDAWHLILQGAAQKETSARQPVKLGEAAS
                     GVIPSQALKAVTLSEKV"
     gene            801..1745
                     /gene="fmt"
                     /locus_tag="VLD60_11265"
     CDS             801..1745
                     /gene="fmt"
                     /locus_tag="VLD60_11265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSB45585.1"
                     /translation="MHIVFMGTPDFAVPSLEALLKSPDEVVGVVTQPDRPKGRGQVLT
                     PSPVKQVALREHIPILQPTKMKDPDFLAALGAWKPDLIAVAAFGRILPPVILSLPPKG
                     CINVHGSLLPKYRGAGPIQWAIINGETETGITTMLMDEGMDTGAMLLQEKIMIAPDDT
                     AGSLSPRLAELGGRLLVETLTRLKAGTLIPQVQDHAQATMAPLLKKEDGAIDWALSAT
                     AIANRVRGLTPWPGAYTFLGEDRWMLYRAVALTGASAEPPGRITAVTKDAIQVATGEG
                     LLAVKELQPANSRRMTVAQYLAGHPIQAGLQLGQQAIL"
     gene            1775..3205
                     /gene="rsmB"
                     /locus_tag="VLD60_11270"
     CDS             1775..3205
                     /gene="rsmB"
                     /locus_tag="VLD60_11270"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HSB45586.1"
                     /translation="MPTGPRSSPASRVPQSARAVALAVLVETVKSEEGVDVLLDRRLE
                     PCSFDSRDRGLAVELTYGVLRRFGTIDWRLEPVLDKPLPRLPIMVQMLIRLGAYQLLF
                     LDRIPQSAAVNESVQLARALERALGRDWSGFVNAVLRQLIREPAPPWPCMETHAAKAL
                     AVRYSVPEWMSQRWIDRLGAATAERICEEVSAIPPVTLRVNRTKIGRDEFLAQLQRAG
                     IVAKPTSVSPVGIMIENAGPIPALPGFQEGLFYVEDEAAQLIPPLLDPQPGDVILDAC
                     AAPGGKATHMAELLRDRGVIYAIDRKGARLDLLRQNCARLGIQSVLPITGDVRHPSEW
                     IRAVEAVGRGAQKRKALFDRILVDAPCSGLGVLRRHPEAKWRKDCAAFSRHHTLQLEI
                     LGSVAPRLRPGGVLVYSTCSTEPEENEAVIEQFLSTHAGFRRESVAPWLPTDARSFLT
                     ERGDLSTLGNRLSMDGFYAARLTKVE"
     gene            3205..3885
                     /gene="rpe"
                     /locus_tag="VLD60_11275"
     CDS             3205..3885
                     /gene="rpe"
                     /locus_tag="VLD60_11275"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSB45587.1"
                     /translation="MARRILIAPSILSADFARLAEEVGAVERGGADLLHVDVMDGHFV
                     PNLTVGPPIVESLKKVTKLPLDVHLMMTNADAFIAEFAAAGADYLTVHVEACPHLHRT
                     VQSIRERGVKAGVTLNPATSLHTIEEILPEVDLVLIMSVNPGFGGQKFIASCLRKVAA
                     ARQMIDRASSRALLEVDGGVKIDNAAQVLAAGADVLVAGSAIFSSRDYAATIAALRAA
                     GQPASANR"
     gene            3895..5460
                     /locus_tag="VLD60_11280"
     CDS             3895..5460
                     /locus_tag="VLD60_11280"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSB45588.1"
                     /translation="MDISSLIDSLHPLEIKVLMAFGAKPSGATLDSEQLAAATELDAS
                     QLSMAVEWLLAKSLLAIDKETVTPIVSLTNVGEQYYEKQSPIEQVLAAARGVAGTGQR
                     LTIQDLQSTEGLDPSDISKAVGRLKKEGVLLIVQGGCIESTGRPSPTVEQVRGLLQQL
                     HEGPRELENFPEAERQVIQDYAVKRGNAKEPFRVDDRVARSYKLSPAGVTAADHLAKQ
                     GVAEEVSQLSPELLKDGSWRNKRFRKYTISLRAPRIGTGKKHPYREFLDTVKTKLVSM
                     GFQEMRGSLVETEFWNMDALFMPQFHPARDIHDVYFVKEPTHATRIAEPFLSRVTKAH
                     QNGGSTGSTGWGYPFDGERAKRLVLRSQGTAVSARTLASAPAVPGKYFSIARCFRYDQ
                     VDATHATDFFQVEGIVLGEDINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVE
                     MHVRHPRLGWMELGGAGLFRPEVTLPLGVTVPVIAWGLGLDRMAMVALGIHDIRELFT
                     DSLDLIRTTKGLF"
     gene            5478..7196
                     /gene="pheT"
                     /locus_tag="VLD60_11285"
     CDS             5478..7196
                     /gene="pheT"
                     /locus_tag="VLD60_11285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSB45589.1"
                     /translation="MPTISIFKTDFESLLAGQKRARRSVSLEQLEEWLMLVKGELKGH
                     NPETGELRVELQDSNRPDLWCCEGIARQVRIKQQGKSAPYSFLKRKPSPAQRMIVAPG
                     MERVRPYVAACTARGYTVTDEGLAQLIQTQEKLADIFGRKRRTVSIGLYRLAQIQFPV
                     TYDLVKPEEAKFTPLGMETEMTLSEILLVHPKGLEHGHILEGLDRMPLLRDAKDRVLS
                     FPPIINSREVGEVRIGDEELFVEVTGTDLSMVVLTLNILAANLADRGATIEPIEVRYP
                     YKTSLGKSVVTPQDLGKPRTLPVKTIEQALGQPIGVKGVRQALEVYGYDVSVGKGTVK
                     AKLPAYRQDLMHAMDVVEDVAISRGYADFQPVMPAQFTVGGLSRIEQLSDRARELMVG
                     LGFQEIISNILGSPDAYRSAMRLDDTEWGRMVEVGNVMSLSFSCLRQWMIPSLLRVEA
                     ASNRAFYPHRIFEAGEVAIPDRSQEFGSRTDTVLGAVIAHATAHFSEIHSCLDVLFYH
                     LDRPYRLEPLTHPAFLEGRVGRIVAGEVAVGMIGELHPEVLERWQIAVPAVAFEINLS
                     QLTAPL"
     gene            complement(7275..7643)
                     /gene="rplT"
                     /locus_tag="VLD60_11290"
     CDS             complement(7275..7643)
                     /gene="rplT"
                     /locus_tag="VLD60_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSB45590.1"
                     /translation="MPRAKGGPKTRARRKKRLKLAKGQYGAKSRLFRSATESVDKGQQ
                     YAYTGRKNRKRDFRQLWIARISAATRAHGLTYGRFINALKKANVLLDRKVLSDMAIKD
                     ATGFEKLVGLAKQHLTPATA"
     gene            complement(7685..7879)
                     /gene="rpmI"
                     /locus_tag="VLD60_11295"
     CDS             complement(7685..7879)
                     /gene="rpmI"
                     /locus_tag="VLD60_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSB45591.1"
                     /translation="MKVKSHSGASKRFGKTGSGKFTRKKAGKRHILTSKNRERKRRLS
                     GSVVVDPTKTKALSRLLPYI"
     gene            complement(7923..8414)
                     /gene="infC"
                     /locus_tag="VLD60_11300"
     CDS             complement(7923..8414)
                     /gene="infC"
                     /locus_tag="VLD60_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSB45592.1"
                     /translation="MNREIRVREVRVIGPEGEQLGILPTPEAFRQAQENGYDLVEVAP
                     TSVPPVCRIMDYGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIKIRQIKG
                     FLEEGNKTKVTLTYRGREMANQEMGRTLMNSVIAELVEAGTIEYAPRMEGRSLIMIVA
                     PKA"
     gene            complement(8443..10377)
                     /gene="thrS"
                     /locus_tag="VLD60_11305"
     CDS             complement(8443..10377)
                     /gene="thrS"
                     /locus_tag="VLD60_11305"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSB45593.1"
                     /translation="MPKITLKNGSSYDVPAGQTVAQALAAAGVPVGPDLLAAIVNGHT
                     TDLSAVLTSDSTIEPLRFDSPEGREVYRHSSTHIMAQAVKELFPTAQLTIGPALEDSF
                     YYDFAFERPFTPEDLEKIEARAIEIAKRKLTITRREFTKQEAVAFFKSRREDYKVELI
                     QGFPDGEPISVYAQGEFVDLCRGPHLPSTGHVGAFKLLTTAGAYWRGDERNPMLQRIY
                     GTSFPTKAELDAHLARLEEIKRRDHRKVGKELDLISVQDEIGPGLVLWHPKGAVIRLL
                     IENFWREQHIKDGYELVYSPHVARLDLWKTSGHVDYYRENMFAPMKLEGSEYQLKPMN
                     CPYHIMIYKSHLRSYRDLPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLFCRPD
                     QIETEVSRVLDFTFFILRTFGFSEFEVFLSTRPKESVGADEHWTLATSALEAALKGRN
                     ISFRLDPGGGAFYGPKIDIKIKDALGRSWQCSTVQVDFNNPERFELSYIGEDGKSHQP
                     IMIHRALMGSIERFFGILIEHYGGAFPTWLAPVQAIVMAITDHQRDFVGKVVAEMKAA
                     GFRVEADLRNEKIGLKIREAEKAKTPFMFVVGDREVQSGTLSVRGRSGANLGSMTVAG
                     ALDLLRAEVNRAGQQLSPTS"
     gene            complement(10542..10616)
                     /locus_tag="VLD60_11310"
     tRNA            complement(10542..10616)
                     /locus_tag="VLD60_11310"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10582..10584),aa:Val,seq:gac)
     gene            complement(10752..11024)
                     /locus_tag="VLD60_11315"
     CDS             complement(10752..11024)
                     /locus_tag="VLD60_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSB45594.1"
                     /translation="MTKEELIAKMAASAGITKVAAGTALQAFTGAVTSSLKKGQRVSL
                     VNFGTFTISKRKARMGRNPRTGEALRIPAAKVPKFSAGKVLKSAVR"
     gene            complement(11124..11714)
                     /locus_tag="VLD60_11320"
     CDS             complement(11124..11714)
                     /locus_tag="VLD60_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB45595.1"
                     /translation="MTLSSSVFSILLLSGDSDLQSQFKQAFKDAAMTTAKDVAALPRE
                     AAKRTFDAVIVESKPGSQGSSHLLTGHVDVTDALVITGSRPALRRASKFMQAMGRLKS
                     APTNGAPAPSLEDYLDWKMKDFVKGMRNGSGRNLHPMLISAIERPLIVRALQETKGNQ
                     IQAAELLGLNRNTLRKKIQDLHIPVKRSRGAEARDG"
     gene            12124..12573
                     /locus_tag="VLD60_11325"
     CDS             12124..12573
                     /locus_tag="VLD60_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB45596.1"
                     /translation="MKVSLRSTYGIMAAVDLAMHIESAPVQAKSIARRQGIPARFLEQ
                     VLHAMKKAGLVSSHRGAQGGYLLSKKPSELSVAEILEALDGPFSSGPGLNGHSSVRRP
                     TKSEVLLAKVWDRVGAAERDVLDAITVEELAGQQRQLEQQRSLMYHI"
     gene            12581..15064
                     /locus_tag="VLD60_11330"
     CDS             12581..15064
                     /locus_tag="VLD60_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSB45597.1"
                     /translation="MSQLSTTNVAVRTEPIPPAILEEIETFEKEALRMLSGEIVNDLF
                     KPFRLQYGIYGQRQPGVQMVRIKIPFGGLTANQLRRVAELAERFATGVGHVTTRQDIQ
                     LHFVELKDVPTIMRGLAEVGLTTREACANTVRNVTACHLAGVCQGEVFDVTPYAKTVA
                     YHLLRNPLNQSLPRKFKIAFSGCKHDCALTPIHDIGLLAARKEDGAIGFRMVAGGGLG
                     AAPRIAQVLREFTPIDELIPSIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFAEFKRR
                     WEEAYVAMGHARPTNEPLRVLSHRDEPVPLIMPTSNGNGATRTAVQGNGHPDGGRQES
                     PFEMWKRTNVVRQKQSGYVAAVVKLFMGDLTAEQMLHIADLADRYSNGNIRTTINQNM
                     IIRWIPEAQIADLYGDLASKGLSDPGAELAEDIIACPGTDTCGLGITSSKGLARALAE
                     VFPAGRVPEDLADVSVKISGCHNSCAQHHIATIGLHGVGKRIGEHTAPHYELHLGGRV
                     NGTAKIGQMTVKLPAKAVSAAITHLIDVYRRDRKSGEGLPAFIDRVGKNALKDELIPY
                     TIVPAFADDPTFYYDWEGEEEFVLEDLGPGECAGGALEMIENGILEADQELYQAKLLA
                     DNHQYAVSVNKSYRAVLAAAKALLVTEGLEPSTDSETFIEFDRRIAQKGVVPAIYREL
                     NKQVGDLGPKDTTAESARDKMAFAKGFVDACRAATDQMGKDLKLAQATEEKKPAAVIP
                     EAKPAAPAPTGAPVYDLRGVACPLNYVKTKLKLEMMDAGEKLEVWLDAGEPIKNVPMS
                     LKNDGHLVLLQEPLEPEAAHYRVLVEKVE"
     gene            15082..15789
                     /locus_tag="VLD60_11335"
     CDS             15082..15789
                     /locus_tag="VLD60_11335"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSB45598.1"
                     /translation="MTKLSVEEIQSWGESFEDKQPQEVLAAAIERYAPKIVLACSFGA
                     EDVVLVDMVHRINPSVTLFYLDTDFLFPETYATRDRIIKQYGLQSAQVLQVKSLLTPE
                     HQAEQHGAALWASDPDRCCQLRKVEPLTRVLKGFEAWITGIRRDQAPTRANAKLIEWD
                     AKFQLVKVNPLAKWTWADVWTYIKVYEVPYNELHDRNYPSIGCTHCTAPVMPGEDPRA
                     GRWKNNAKTECGLHKAS"
     gene            15789..16850
                     /gene="trpD"
                     /locus_tag="VLD60_11340"
     CDS             15789..16850
                     /gene="trpD"
                     /locus_tag="VLD60_11340"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSB45599.1"
                     /translation="MPMQEFIAKIAKGQKASKDLTWDEAKRAMKALIEGDATPAQAGA
                     FFVAMRIKMESVTELASFTAAARSYVAPVPIPRELGVVDLPSYAGKQDTFHALAGAAI
                     VAAAAGASILMHGYDGIPGRPGNAGVLKALGLPIDLEPKAAADVVMKQGFAYLDIALY
                     HPPLYRFLEMREELGVRNVSHPIARLLNPARASSQVVGLTHPPHFEKTAEALRMLGTP
                     RALVVRGVEGDPELSIAMVTKMLELRDERIAPLTLAPKDVGLALGASREMAGFAPEQR
                     DKETELLKRILHNQVQGGPRDWVLLNAALLLYAAGKGSSVAACVPLARRTIDEGAAAR
                     KLEELNREPVASGAAGRIH"
     gene            16872..17669
                     /gene="cysD"
                     /locus_tag="VLD60_11345"
     CDS             16872..17669
                     /gene="cysD"
                     /locus_tag="VLD60_11345"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HSB45600.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRLAREWHLNLVVGQNKEALAAGMNHTMGRVV
                     CCTALKTNGLKQLLEQKGYTGVILGVRADEEGTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQYKTTFPPGTHIRIHPLLDWTEINIWEYIKLENIPFIDLYLDKGDGTRYRSLGCAP
                     CTTPIKSTAKSVDDIIEELRHTTVAERSGRAQDEGRGMELLRKDGYM"
     gene            17810..19654
                     /locus_tag="VLD60_11350"
     CDS             17810..19654
                     /locus_tag="VLD60_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB45601.1"
                     /translation="MNTAINKPSENLNIVIVGHVDHGKSTLLGRLYADTGSLPDGKLE
                     KVQAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFIWKGRQYIIIDAPGHKEFL
                     KNMISGAARAEAALLLIDALEGVKEQSKKHGYLLSLLGVRQFAVVVNKMDLVGYRQDV
                     FEGIEKEYREFLGQFGAVPERIIPVSAKLGDNIANRSQAMPWYNGPTVLDTLSLFKKE
                     MVRSEQPLRFPVQDVYKFDARRILAGRIVAGRLKIGDHLVFSPSNKRANVRSVEAFNI
                     EPSPTGAEAGQSVGITLDEQIFVERGEIATHQEHLPLVSTAFRANLFWLGRRSLEKGR
                     KYVLRIGTKEVDCEVAAIHRIIDTMDLNQQQGSSSVGRNQVAELTIRTKAPMAFDLSA
                     SFEATGRFVLVDEYDIAGGGIVTELVHDDQEFLREEARRRDFAWVKGEVGIEERAQQY
                     GHRAAIVLVTGGRHTGKSFLAKTLEARLVADGRHAYLLDGENLRRGLDADLSEGERGQ
                     ATEMARRYGEVARLLVDTGLIVVSTTNPFGMSYIEAAQAIRTLVHPTPVISVHMSKVP
                     EEVPPNTDIVLSGPSDFDAATRRVLDELKRRGVLAQAIGAKPTFQYSI"
     gene            19780..20700
                     /locus_tag="VLD60_11355"
     CDS             19780..20700
                     /locus_tag="VLD60_11355"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSB45602.1"
                     /translation="MAPEKDLKTILGKLRYSDDAKVVQQITEQMKQVQTRMAGIKHKL
                     VVMSGKGGVGKSMTTVNLALAFARQNARVGLLDVDLNGPCVPRMLGLHGQSLTMTPEG
                     AIPPIGPLGIKVASMDFFLGAASPVRWKGPMDVSPVWLGLMEMNVIREFLADVAWGEL
                     DYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIKVL
                     GIVENMSEYRCPSCGELNELFEGNTESMCEALDLPLLGRIPFDRKLARTFDKGEPLLD
                     ETYPTIQRYQEIAGRIRTLLDYKKVLAEQL"
     gene            20723..>21240
                     /locus_tag="VLD60_11360"
     CDS             20723..>21240
                     /locus_tag="VLD60_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSB45603.1"
                     /translation="MKFVCLTCETYMNFEKVEKPGEGSLGVFFGCPSCGAKFSMVTNP
                     GETQMVSSLGVQLGGRTVAAEPFEMTRGTLKDEALAGSGQMAAYLNEKIQGGQPAAAS
                     TAPAPAKAGEKSSGGCPFSAMVAEMGLTSSGKPATSGAGTSEFTWTADAKEKLDRLPV
                     FVKPMVQSSVEA"
BASE COUNT         4512 a         5872 c         6234 g         4622 t
ORIGIN      
        1 ttcgaccttt gggttatgcg taccactcca gctttcgctg tccggcttga ccggtttgtg
       61 gtctggactt tctcttcacc ctcggaccgg caatgccgcc gttagggtgc ctgccattaa
      121 gtctctacac cttctctgag agcgttaccg ctttcagagc ttggctcggg attaccccgc
      181 tagcggcttc cccgagtttg acaggttgtc tcgcagaagt ttccttctgc gctgcccctt
      241 gcaggatcaa gtgccaggcg tcccgataag cggcggaacg cggcttccgt tgccgcttct
      301 cagtttccac cagatgaatc tcgctggcgt agctgatgaa cgcgtcaaca ggaaatacgt
      361 agaacaagtc ttcggccgga aggtacgcca gcgcaaaatc aaaatcggaa ggggtataga
      421 cctcccgaat catgacccgc cggttcgtct tggtccgacg attatcgatc acgaaatttc
      481 ctcgtgactc gtcgaaccac gcgcacttca cctgaacctt gatgagagtt ccctggatat
      541 cgaagaccag atcgtaaggc aaccgatctc cgataggctt gagaattccc ccgccacgct
      601 tcagcccctg aaggatggct gcttgctctg cgatatctcc tttgagtttt gtactcacgg
      661 agatcctgcg gattgagccc aacgagctac caggctgctc caccccgcga gcggatgcta
      721 acaaagcgat cgtagcagag tcaaggtaaa cttcctcgcc gttggttgct ttcactcagg
      781 ctagcgggta aaaagtgtcc atgcatattg tgttcatggg cacgccggac tttgcggttc
      841 cctctctcga agccctgttg aaatcacccg acgaagtggt cggcgtcgtt acgcaacctg
      901 atcgacccaa aggccgcggt caggtcctca cgccttcacc ggtcaagcaa gtcgcgctgc
      961 gcgaacacat tcccattctg caaccgacta agatgaaaga tcctgacttt cttgcggcgt
     1021 taggtgcatg gaagccggat ctgatcgccg tcgcggcatt cggccgaatc ctgccgcccg
     1081 tgatcctttc gcttccgccg aagggctgta ttaacgtcca tggttcgctc ttgccgaagt
     1141 atcgcggcgc cggcccgatt caatgggcaa tcatcaacgg cgaaacagaa accggtatca
     1201 cgacgatgtt gatggatgaa gggatggaca cgggggcgat gctgttacag gagaagatca
     1261 tgatcgctcc cgatgacacg gccggcagtt tatctccgcg gctcgccgaa ttgggtggtc
     1321 gattgctcgt cgagacgctc accagattga aggcgggaac gttgattcct caagtccaag
     1381 accatgctca ggccaccatg gcgccgctgc tgaaaaaaga agatggcgcg attgactggg
     1441 cattatcggc cacagctatt gccaaccggg ttcgcgggct gacaccctgg cccggggctt
     1501 atacatttct cggagaagac cggtggatgc tgtaccgggc cgttgcgctg acaggggcga
     1561 gcgcggagcc tcccggccgg atcaccgcgg tgacgaaaga cgcgattcaa gtggccacgg
     1621 gagaaggcct ccttgccgtg aaggagttgc agcctgccaa tagtcgtcgg atgacggtcg
     1681 cgcaatacct tgccggccat cctattcaag ccggattgca gttgggtcag caggccattc
     1741 tctaatagct cgattcacag aagtttgagt gcccatgccc actggtcctc gatcctcacc
     1801 agcttcccgc gtacctcagt cggctcgtgc ggtggccctg gccgtcttgg tggaaactgt
     1861 caaatccgaa gaaggggtgg atgtcctcct ggatcgaagg ctcgagccct gctcattcga
     1921 cagcagggac cgcggtctcg ctgtcgagct gacatacggg gtgctacgtc gctttgggac
     1981 gatcgattgg cgcctcgagc cggtgctcga taaaccactc cctcgtctgc caatcatggt
     2041 gcagatgcta atacggctcg gggcgtatca gttgcttttc ttggatcgga ttccgcagtc
     2101 cgccgcggtt aatgagtcgg ttcagctcgc aagagcactt gaacgagcac tggggcgtga
     2161 ttggagtggc ttcgtcaacg cggttctgcg tcagctcatt cgggagccgg ccccgccatg
     2221 gccttgcatg gagacccacg cggcgaaggc tcttgccgtg cgctattcgg ttccggagtg
     2281 gatgagtcag cgctggatcg atcgtcttgg tgcagcgacg gcggaacgaa tctgtgaaga
     2341 ggtgagcgct attccgcctg tgacgctcag ggttaatcgc accaaaatcg gcagggacga
     2401 gtttctggct caattgcaac gggccggaat tgtggcaaaa ccaacttccg tcagccccgt
     2461 cggcatcatg atcgaaaacg ccgggccaat tccagctctg ccgggatttc aggaagggct
     2521 gttttatgtg gaagatgagg cggcccagct gattcctccc ctactcgacc cccagccagg
     2581 tgatgtcatt cttgatgcct gtgccgcgcc aggtgggaaa gcgactcaca tggccgagct
     2641 gctgcgcgat cggggggtca tctatgcgat cgatcgaaaa ggtgcacgcc tggatctcct
     2701 gcggcagaac tgtgcgagac ttgggattca gagcgtcctg ccgatcaccg gcgacgttcg
     2761 tcatccctca gagtggattc gtgccgttga ggctgtgggg cgaggcgcgc aaaagcgaaa
     2821 ggcgttgttc gatcggatcc tggtggatgc tccttgcagc ggccttggcg tgttacggcg
     2881 gcatccggaa gcgaagtggc ggaaggattg tgccgcattc tcgcgccatc acaccctgca
     2941 gctggaaatt ctcggatcag tggctccacg cttgcggccc ggcggggtgc tggtctatag
     3001 tacctgttcg acggaaccgg aagagaacga agccgtcatt gagcaattct tgagcaccca
     3061 cgcggggttc cggcgcgagt ccgttgcgcc ttggctcccg acggacgcac ggagttttct
     3121 caccgagcga ggggacctct cgacgttggg caaccgcctc tcgatggatg gattctacgc
     3181 cgcgcgtctc acgaaagttg agtaatggcc agacgaatcc tcattgcccc atcgatctta
     3241 tcggctgatt ttgcgcgttt ggctgaggaa gtgggtgccg tagaacgtgg cggcgccgat
     3301 ctcctgcatg tcgatgtcat ggatgggcat tttgtgccca atctgaccgt gggcccaccg
     3361 atcgttgagt cgcttaagaa ggtgacgaaa ctcccgctcg acgtgcatct gatgatgacc
     3421 aatgccgatg cctttatcgc cgagtttgcc gcggccggcg ccgactatct gacggtgcat
     3481 gtggaggcct gtccccacct gcatcggacg gtacaatcca tcagggaacg cggggtcaag
     3541 gccggcgtta cgctcaatcc ggcgacgtct ctccatacca tcgaggagat tcttcctgaa
     3601 gtggatctgg tcctcatcat gtcggtgaat cccggatttg gcgggcagaa gttcatcgcg
     3661 tcatgtctcc ggaaggtggc tgcggcacgt cagatgattg accgggcgag cagtcgtgcg
     3721 ctgctcgaag tcgacggcgg cgtgaagatt gataatgctg cgcaagtgct ggcggccgga
     3781 gccgatgtgc ttgttgccgg atccgcaatt ttttcaagcc gagactatgc cgccacgatt
     3841 gccgcgctcc gggcggccgg gcagccggct tcagcgaacc gataggctct tcgggtggac
     3901 atttcttccc tcatcgacag tttgcatccc ctcgaaatca aggtgctcat ggccttcggg
     3961 gcgaagccgt ccggggcgac acttgattcg gagcagctgg cagccgcgac ggagctcgat
     4021 gcctcgcagc tcagcatggc ggtggaatgg ctgctggcca agtcgctcct cgccatcgac
     4081 aaggaaacgg tgacccccat cgtgtcgctg acgaacgtcg gcgagcagta ttacgaaaag
     4141 cagtccccca ttgaacaggt gcttgcagcg gcgcggggtg tggccgggac gggccaacgg
     4201 ctgaccattc aggatcttca atcgacagag ggactcgatc cctccgatat cagcaaggcc
     4261 gtcggccgcc tgaaaaagga aggcgtgctc ctgatcgtcc agggcggctg tatcgaatcg
     4321 acgggacgcc cgagtccgac agttgaacag gtgcgagggc tcttgcagca attgcatgaa
     4381 gggccccgcg agctggagaa ttttcctgaa gccgagcggc aggtcatcca agactatgcg
     4441 gtgaagcgtg gtaacgccaa ggaaccgttc cgtgtcgacg atcgcgttgc caggtcctac
     4501 aaactatccc ctgcgggcgt gacagcggcg gatcaccttg ccaagcaagg ggtggctgaa
     4561 gaagtatcac aactcagtcc tgaactgtta aaggacggaa gctggcgtaa caagcgcttc
     4621 agaaaataca ccatcagttt gcgcgcccct cgcatcggga ccggcaagaa gcatccctat
     4681 cgagagttcc tggatacggt gaagacgaaa ctcgtcagca tgggatttca ggaaatgcgg
     4741 ggatcgctcg tcgagactga attctggaac atggacgcgc tcttcatgcc gcaattccat
     4801 cccgcgcgcg atattcacga cgtctacttc gtgaaagagc cgacccatgc gacgcgcatc
     4861 gccgaaccgt tcctgtcgcg cgtgaccaaa gcccaccaga atggcggcag taccggctcg
     4921 accgggtggg ggtacccctt cgatggcgaa cgggccaagc ggttggtgtt gcggagccaa
     4981 ggaaccgccg tatccgcaag gacgctggca tcggccccag ccgttccagg gaaatacttt
     5041 tccatcgcgc gctgcttccg ctacgaccag gtcgatgcga cccatgcgac ggattttttc
     5101 caagtggaag gaattgtgct cggggaagac atcaactttc ggacgttgct gggcctcttg
     5161 aacttgtttg cgcgcgaggt tgctcaagcg aaagaggtga aatttctgcc ggcctatttc
     5221 ccgtttacgg aaccttccgt cgagatgcat gtccgacatc cccgtttggg gtggatggaa
     5281 ctcggcggcg ccgggctgtt caggccggaa gtcacgctgc cgctcggcgt gacggttccg
     5341 gtcattgcct gggggcttgg gttggaccgg atggcgatgg tggccctggg cattcacgac
     5401 attcgcgaat tattcaccga cagtctcgat ttgattcgaa cgacaaaagg attgttctag
     5461 gcgacctgct gggtcttatg ccgacgatct cgatcttcaa gacggacttt gaatcgcttc
     5521 ttgccggcca gaagcgagcc aggcgatcag tctcgctcga gcagctcgaa gagtggttga
     5581 tgttggtgaa gggcgaactc aagggacaca accctgagac gggcgaactc cgcgttgagt
     5641 tgcaagatag caatcggccg gatttgtggt gttgtgaagg gatcgcgagg caggttcgaa
     5701 ttaagcagca gggcaagtcc gctccctatt cgtttctcaa acggaagccc agtcctgccc
     5761 aacggatgat cgttgctcca gggatggagc gcgtccggcc atatgttgcg gcctgcacgg
     5821 ctcggggtta cacagtcacc gatgaggggc tggctcaact cattcaaacg caagagaagc
     5881 tcgcggatat cttcggtcgg aaacgtcgga ccgtctcgat cgggctctac cggctggctc
     5941 aaatccagtt cccggtgacg tacgatctgg tcaaaccgga ggaagcgaag tttacccccc
     6001 tagggatgga aaccgaaatg accctttcag aaattcttct ggtccatcca aaaggactcg
     6061 agcacggaca tattctcgaa gggctggatc ggatgccgct cttgcgagat gccaaggacc
     6121 gagtcttgtc gtttccaccg atcatcaaca gccgagaagt cggagaggtc cggattggcg
     6181 atgaggagtt gtttgtggag gtgaccggca cggatctctc gatggtcgtg ctcacattga
     6241 atattctcgc ggcaaatctg gcggaccgag gcgccacgat cgagccgatc gaagtccgat
     6301 acccgtacaa aacttcattg ggaaaatccg tcgtgacgcc acaggatttg ggaaaaccgc
     6361 gcacgctccc tgtcaaaacg atagagcagg cattaggcca gccgatcggt gtgaaagggg
     6421 tcaggcaggc gctcgaagtg tacggctacg atgtatccgt gggaaaaggg accgtgaagg
     6481 cgaaattgcc agcctaccgg caggacctca tgcatgccat ggatgtcgtg gaggatgttg
     6541 cgatcagtcg tgggtatgcc gacttccagc ctgtcatgcc ggctcaattt acggtcggag
     6601 gcctgtcgcg aatcgagcag ctgtccgatc gagcccgtga actcatggtc ggattgggtt
     6661 tccaggagat catctcgaat atccttggat cccccgatgc ctatcgcagc gccatgcggc
     6721 ttgatgatac cgagtggggc cggatggtcg aggtggggaa tgtcatgtcg cttagttttt
     6781 cctgtctgcg gcagtggatg attccctcat tgcttcgggt tgaagccgcg tcgaaccggg
     6841 cgttttaccc ccaccgcatc ttcgaagcgg gagaggtggc gattcctgac cgctcgcagg
     6901 aatttggctc acgcacggac acggtattgg gagccgtgat cgcccatgcc actgcacatt
     6961 tttctgagat ccactcctgc ctggacgtgc tgttctatca tctcgaccgg ccctacaggc
     7021 ttgaaccctt aacccatcct gcgttccttg aaggacgcgt gggaagaatc gttgctggtg
     7081 aagtggccgt cgggatgatc ggagaactgc accccgaggt gctggagcga tggcagattg
     7141 cggtgcccgc ggtggcgttt gaaatcaatc tttctcaact cacagcgccg ctgtgaggag
     7201 ggaagcgacg gtctgtcgct tccctttccc gttcacatca gatacagcag aagaaatgga
     7261 ggcgtcccgc gacgctaggc agttgccggc gtgaggtgtt gtttggccag accgaccagc
     7321 ttctcaaaac cggtcgcatc tttaatggcc atgtcggaga gtacttttcg gtccagcagg
     7381 acattcgcct ttttcagggc attgatgaat cggccatagg tgagtccgtg cgctctcgtc
     7441 gctgcgctga tccgcgcaat ccagagctga cgaaaatccc gtttacggtt ctttcgtccg
     7501 gtataggcat actgttgacc tttgtcgacc gactctgtgg ctgaacggaa caaacggctc
     7561 ttggcaccgt actgcccttt cgccagtttc agccgctttt tccgtctcgc tcttgttttt
     7621 ggcccacctt ttgcgcgtgg catgactcaa tactccttgt tagttgcgat gcatcaaccc
     7681 atctctagat atacggcaac aggcggctga gggccttggt tttggtcgga tctacgacca
     7741 ctgacccgct caagcgccgt ttacgctccc gattcttgct tgtcagaata tgtcgcttgc
     7801 cggctttctt ccgggtgaat ttccccgatc ccgtcttgcc gaatcgcttg ctggctccac
     7861 tgtgtgactt aactttcatc gtctggtcct ttggttaaac gttcgtcgtt ctatgctctt
     7921 gttcaggcct tcggagccac gatcatgatg agactccggc cttccatacg gggggcatac
     7981 tcgatggtac cagcctccac caactccgcg atgacagagt tcatgagcgt ccgtcccatc
     8041 tcttgattgg ccatttcgcg cccgcgatac gtcaaggtga ccttcgtctt atttccctct
     8101 tccaagaagc ccttgatctg gcggatcttg atttccagat cgtgcttgtc cgtccgaggg
     8161 cgtaatttga tctccttgac ttgtgtcgac ttctggtgcc gtcggctctg atggtccttc
     8221 ttgctcaact catacttata cttcccgtag tccatgatac gacagacggg aggaactgac
     8281 gtcggcgcga cctcgacgag gtcataaccg ttctcctgcg cttggcggaa tgcttccgga
     8341 gtcgggagaa taccgagttg ctcaccttca ggaccaatga ccctgacttc acgaactctg
     8401 atttcgcggt tcacgcgcag tttggggacg ataggccacc tctcatgatg tgggtgaaag
     8461 ttgctgcccg gcccggttca cttcggccct gagcagatcc aacgcgccgg cgacggtcat
     8521 gctgccgaga ttcgcaccgc tgcgtcctct cactgacagc gtgccgctct gaacctcacg
     8581 atcgccgacg acaaacatga acggagtttt cgctttttca gcctcgcgaa tcttgagccc
     8641 aatcttttcg ttccgcaaat ccgcctcgac tcggaagccg gccgccttca tttcggcgac
     8701 caccttgccg acgaagtcgc gctggtgatc cgtaatggcc atcacgatgg cctgaacggg
     8761 ggccaaccag gtggggaatg ctcccccgta atgctcgatc agaatgccga agaaccgttc
     8821 aatcgatccc atcaaggctc ggtggatcat aatcggctga tgagattttc cgtcttcgcc
     8881 gatgtaggat agttcgaatc gttcaggatt attgaaatcg acctggacgg tcgaacattg
     8941 ccacgaacga cccaaggcat ccttgatctt gatatcgatc ttggggccgt aaaatgcgcc
     9001 tcctcccgga tccaggcgga aggagatatt gcgtcccttc agggctgctt ccagcgcgct
     9061 ggttgcgagc gtccaatgct cgtcggctcc caccgattct ttcggtctgg tcgaaagaaa
     9121 cacttcgaat tcggaaaatc caaacgtccg taaaatgaaa aacgtaaagt ccagaacgcg
     9181 gctgacttcc gtttcgatct ggtccggacg gcagaacaga tgggcgtcgt cttgtgtgaa
     9241 tccccgcacg cgcaacagcc catgaagaac gccggtccgt tcataccggt agaccgttcc
     9301 caactcgcca tagcgtatcg gtagatcgcg gtaactgcga aggtgggact tgtaaatcat
     9361 gatgtggtag gggcagttca tcggcttgag ctgatactcg ctgccttcca gcttcatcgg
     9421 ggcgaacata ttttcacggt agtagtcgac atgtccgctg gtcttccaaa gatcgagtct
     9481 ggccacatgg ggtgaataga cgagttcgta accgtcctta atatgctgct cgcgccagaa
     9541 attctcgatc agcaatctga tcacggcgcc ttttgggtgc caaagcacga gaccgggtcc
     9601 gatttcatct tgaaccgaga tcagatccag ctctttcccg actttccgat ggtcccgccg
     9661 tttgatctct tccaaacggg ccaagtgcgc atcgagctcc gccttggtcg ggaaagatgt
     9721 tccgtagatc cgctgcagca tcgggttgcg ctcatcgccc cgccagtaag ccccggcggt
     9781 agtgagaagc ttgaatgcgc cgacatggcc ggtcgatggc agatgcgggc ctcggcagag
     9841 gtcgacaaac tcaccttgcg catagacaga aatgggctcc ccgtcgggga agccttgaat
     9901 caactctact ttgtagtctt ctcttcgtga tttgaagaag gcgacggctt cctgcttggt
     9961 gaattcccgt cttgtaatcg tgagcttgcg tttggcgatc tctattgcac gcgcttcgat
    10021 tttttccaaa tcctcaggcg taaaagggcg ctcgaaggcg aaatcatagt agaagctgtc
    10081 ctccagcgcc ggtccgatcg tcagctgtgc cgtcgggaac agttccttga ccgcttgcgc
    10141 catgatatgg gtgctgctat ggcggtagac ctcccggcct tccgggctat caaagcgaag
    10201 cggttcgatg gtcgaatcgc ttgtcagaac cgccgacagg tcagtggtgt gaccattgac
    10261 gattgccgca agcagatccg ggccgacagg gacgccggct gctgccagcg cttgagcgac
    10321 ggtctgcccc gcaggcacat cataactcga cccgttcttc agcgtgatct tcggcacgtg
    10381 accccgtttg cgttgatgat tggcgcagcc cgcaccactc ccccgcgctg cggggtgcca
    10441 ccgcagcaat ccggaggaaa caaaatcctg tatctccagc aggatacaaa aatacgaaag
    10501 gcaccccaac cgttcgagac ggccagatgc ccccggcgag atggtaggcg cggacggtct
    10561 tgaaccgtcg acctctaccg tgtcaaggta gcgctctccc cctgagctac gcgcctatcg
    10621 tccgaacgcg catgctaata taggcccgcc caaggtgtca agaaacgcaa aaaagagaag
    10681 ggagcgtgcc aagattcagg gcacgccgcc ttctcttctt gatcacgcac gaaattggct
    10741 gttagcgatg actaccgcac agccgatttc aacacttttc ctgccgaaaa cttgggcacc
    10801 ttcgcggcag ggatccgtaa ggcctcacca gttctcggat tacggcccat ccgcgctttc
    10861 cgcttcgaga tggtaaaagt cccgaagttc accagagaga cccgctgtcc ctttttcaag
    10921 gatgaggtca ctgcacccgt aaaggcttga agtgcagttc ccgcagccac cttggtgatt
    10981 ccggcggatg ctgccatctt cgcaatcaat tcttctttcg tcattatccc cctcctgttg
    11041 attaggttaa ggaaaccaac tgcccccact tgctgctacg caggatgttg atgcctgctc
    11101 gatacgtaca gaaatgatcc gatttagccg tcgcgggcct ctgcgccccg gctccgcttc
    11161 acgggaatat gtaggtcttg gatttttttt ctcaacgtat tgcggttcag acccaacagt
    11221 tcggctgctt ggatctgatt gcccttggtc tcttgaaggg ctcgtacgat cagcggtcgc
    11281 tcgatggcgg aaatcagcat ggggtgcaaa ttgcggcccg agccgttacg catacccttc
    11341 acaaagtctt tcattttcca gtccaggtag tcttcaagcg acggcgccgg cgctccgttt
    11401 gtgggcgcgc tcttgagccg acccattgcc tgcatgaact ttgacgcccg tctgagggca
    11461 gggcgggagc cggtaatcac caaggcatct gtgacatcga catggccagt aaggagatgg
    11521 gaacttccct gcgatccagg cttggattcc acgatcacgg catcaaaggt tcgcttggcg
    11581 gcctctcgtg gcaatgcagc aacgtccttc gccgttgtca tggcggcatc tttgaaggct
    11641 tgtttgaatt ggctctgtag atcggaatcg ccgctcaaca atagaatact gaagactgaa
    11701 gacgagagcg tcatggagat gtcaccagaa gaagaggccc ggattattgc ccagagccga
    11761 tttgatgtca atgaagaaat tgacggattc atgcagacac gtcagcagac gcacaggata
    11821 gcacttgaga tgctgcacct tcgaatgaat ttgtgccgag tggaagtcag gcagatacca
    11881 gatgaatcgg aggacaggtg gtgcttccaa cttctctgtc gtcgatgcgg aagcttcttc
    11941 acttttaaga gcgagggcga ttattgctcg cgggaggaaa cggtcggtcc aattgatggt
    12001 tgcggcgtga gatgagagga cgcgactctc gtagctcctg aaccaggcaa agcaagggct
    12061 tgacagcaaa gcaaggtttg aagtattagt gtgtttctct aattccgacc ggaatactat
    12121 tgtatgaagg tatctcttcg atctacttat ggaattatgg cagctgttga tcttgccatg
    12181 catatcgagt ccgcaccggt ccaagccaaa tccatcgcga ggcggcaggg gattccagct
    12241 cgattcttgg agcaggttct ccacgccatg aagaaagccg ggctggtgtc gagtcacagg
    12301 ggtgcgcagg gtggctatct gctgtccaaa aaaccttctg aattgtcagt ggcagaaatt
    12361 cttgaggcgc tcgatggacc attttcctcg ggccccggat tgaacgggca cagttctgtc
    12421 aggcgcccca ccaaatccga agtcttactc gcgaaggtgt gggatcgtgt cggcgctgca
    12481 gaacgggatg tgcttgatgc gatcacagtt gaagaactcg cgggccaaca acgccaactc
    12541 gaacaacaac ggtccctcat gtatcacatc tgaggtcact atgagccagc tatccaccac
    12601 taacgtcgct gttcgcactg agccgatccc tccggccatt cttgaggaaa tcgaaacgtt
    12661 cgaaaaagaa gctctgcgga tgctctccgg tgagatcgtg aacgatctct tcaaaccttt
    12721 tcgactgcaa tacggcatct atgggcaacg tcagcccggc gttcagatgg tccggatcaa
    12781 gattcctttt ggaggactga ccgcgaacca attgcgtcgc gtcgccgagc tggccgaacg
    12841 ctttgcgacc ggtgtcgggc acgtgacgac gcgtcaagac atccaattac attttgtgga
    12901 attgaaagac gtgccaacaa tcatgcgcgg gttggcggaa gtcgggttga cgacgaggga
    12961 agcctgcgcc aatacggtgc gcaacgtgac agcctgtcat ctcgccggtg tgtgccaagg
    13021 tgaagtgttt gacgttaccc cctatgcaaa gacggtcgcg tatcatctct tgcggaaccc
    13081 gctgaaccag agcttgcccc gcaagtttaa gatcgccttt tccggttgca aacacgattg
    13141 cgccctcacg cccatacacg acatcggtct gctcgcggcc agaaaagaag acggagcgat
    13201 cggttttcga atggttgccg gtggtggtct tggggcagct ccaagaattg ctcaagtttt
    13261 gcgtgaattt acgccgatcg atgagctgat tccgagcatc gaggccgtca tcaaggtctt
    13321 cgacacgttg ggaaacagaa aaaatcgaaa caaggcccgg atgaagttcg tgattgagaa
    13381 gctgggtttt gccgagttca agcgtcgatg ggaagaagcc tatgtggcga tgggccacgc
    13441 acgccctacc aatgagccgc tcagagtgtt gtcccaccgg gatgagcccg ttccgttgat
    13501 tatgcctacc tccaatggca atggcgcaac gaggaccgcg gtacagggca atggccatcc
    13561 ggatggaggg cgccaggaat cgccattcga gatgtggaaa agaacgaacg tcgttcggca
    13621 aaagcagtcg ggctatgttg ctgcagtggt caagttgttc atgggggatc tgacagcgga
    13681 acagatgctg cacatcgcgg atctggccga tcgctactcc aacggcaaca ttcgtacgac
    13741 gatcaatcaa aacatgatca tccgctggat accggaagcg cagatcgccg acctctatgg
    13801 cgatctggcc tcgaaagggc tttcagatcc tggcgcggaa ttggcggagg atattatcgc
    13861 ctgccctggt accgacacct gcggtctcgg catcacttct tcgaaggggc ttgcccgggc
    13921 ccttgcggag gtgtttccgg ccggacgggt tccggaagat ctcgcggatg tcagcgtaaa
    13981 gataagtggc tgccacaatt cctgtgcgca gcatcacatc gcgacgatcg gattgcacgg
    14041 agtcgggaag cggatcggcg aacatacggc tcctcactac gaattgcatc tcggcggcag
    14101 agtcaacggg actgcaaaga tcgggcagat gacggtgaaa ttgcccgcga aagccgtttc
    14161 ggccgccatc acgcatttga tcgacgtata ccggcgcgat cgcaagtcgg gtgaagggtt
    14221 gccggcgttc atcgatcgtg tcggcaagaa tgcgctgaaa gacgaattga ttccctacac
    14281 gatcgtgccg gcctttgccg acgatcctac gttctactat gactgggaag gggaggagga
    14341 atttgtcctc gaggatctcg ggccgggtga gtgcgccggc ggcgcgctgg aaatgatcga
    14401 gaatgggatt ctcgaagccg atcaggaact ctatcaggcg aagctcttgg cggataacca
    14461 tcagtatgcc gtctcggtga acaaatcgta tcgtgccgta ctcgctgcag cgaaagcgct
    14521 gttggtgacg gaggggctcg agccttccac cgactccgaa acgttcattg agtttgatcg
    14581 ccgtatcgca cagaagggag tcgttcctgc gatctatcga gaactgaaca agcaggttgg
    14641 cgatctgggt ccgaaggaca cgacggcgga atcagcacgc gacaagatgg cgtttgcgaa
    14701 gggattcgtc gatgcctgcc gcgccgcgac ggatcagatg gggaaagatt tgaaattggc
    14761 tcaggcgact gaggagaaga aaccggcggc cgtcatcccc gaggctaaac cggctgctcc
    14821 ggcgccgacc ggtgcgccgg tctatgatct gagaggcgtc gcctgcccgc tgaattatgt
    14881 gaagacgaag ctcaaactcg aaatgatgga cgcgggcgaa aaactggagg tctggcttga
    14941 tgcgggcgag ccgatcaaga acgtgccgat gagtttgaag aacgacgggc atctggtgct
    15001 gctccaggaa ccgttggagc ctgaagcggc gcattatcgg gtgctcgttg aaaaagttga
    15061 atgagcggaa gcgggcatca catgacgaaa ttgtccgtcg aagaaattca gtcgtggggc
    15121 gaatcgttcg aagacaagca gccgcaagag gtgttggcgg ccgcgattga acgctatgca
    15181 cccaagattg tcttggcctg cagcttcggg gcggaggacg tggtgcttgt cgacatggtc
    15241 catcgaatca acccctccgt gaccttgttt tatctggata cggattttct gtttccggaa
    15301 acctacgcga cgcgggatcg catcatcaaa cagtacggtc tccaatcggc gcaggtgctc
    15361 caagtcaaat ccctgctcac gccagagcat caagctgagc agcacggtgc cgccttgtgg
    15421 gccagcgatc cggaccgttg ctgccagttg cgaaaagtcg agccgctcac gcgcgtgctg
    15481 aagggatttg aggcgtggat cacaggaatc aggcgggatc aggcaccgac gagagccaat
    15541 gcgaaattaa tcgagtggga tgccaagttt caattggtca aggtgaatcc cctcgcaaaa
    15601 tggacatggg cggatgtgtg gacgtatatc aaggtctacg aggttccgta caacgaactg
    15661 cacgaccgca actatccgag catcggctgc acacactgca cggctcccgt gatgccgggt
    15721 gaagatcctc gggcggggcg ctggaagaat aacgccaaga cagaatgcgg gttgcacaag
    15781 gcctcctgat gccgatgcaa gagttcatcg caaaaatcgc caaagggcaa aaagcctcca
    15841 aagacctcac gtgggatgag gccaagcggg ccatgaaggc tctgattgaa ggcgatgcca
    15901 cgccggctca ggctggcgcg tttttcgtgg cgatgcggat caaaatggaa tctgtgaccg
    15961 aactggcgtc gttcaccgct gcggcgagat cttacgtggc ccccgttccg atcccgcgag
    16021 agctcggtgt ggtcgatctg ccctcctatg cggggaaaca ggatacgttc cacgcgttag
    16081 ccggtgcggc cattgtcgcc gccgccgctg gcgcttcgat cctcatgcat ggctatgacg
    16141 gcattcccgg tcgccccggc aacgccggtg tgctgaaggc cttgggtttg ccaattgatc
    16201 ttgagccgaa ggccgcggcc gacgttgtga tgaaacaagg gtttgcctat ctggacatcg
    16261 cgctttacca tcctccgctc taccggttcc tggagatgcg ggaagaactt ggggtgcgaa
    16321 acgtgtctca tcctatcgcc cgactcttga atcctgctcg cgcctcgtcg caggtcgtcg
    16381 gcctcaccca tccgccgcat tttgaaaaaa ccgcagaagc cctgcgcatg ctgggaactc
    16441 ccagagccct tgtcgttcgt ggcgttgaag gcgatcctga attatcgatt gcgatggtca
    16501 cgaaaatgtt ggagttgcgc gacgaacgga tcgcgccgct gacgctcgcg ccgaaagatg
    16561 tcggattagc attgggtgcc tcgcgagaaa tggctggctt cgctcctgag cagcgcgaca
    16621 aagaaacgga actactcaaa cggattctcc acaatcaagt tcaaggtggc ccgcgggact
    16681 gggttttgct caacgccgca ttactcctct atgcggcagg aaaagggtcc tctgtggctg
    16741 cctgcgtacc gctggcgcgt cggacaattg atgagggggc tgctgccaga aaactcgaag
    16801 aattgaatcg ggagccggtg gcatcgggcg ctgccggccg aatccattga ccgaacaaga
    16861 aggacgactc cgtgaagcac ctccgtcagt tggaagatca aagcgtctac atcttaagag
    16921 aagcatataa gcacttcgac aacctcgcta tgctctggtc gatggggaaa gattcgaccg
    16981 ttctcttgtg gctggcacgc aaggcgttct tcggacacgt cccgttcccg ttgctgcacg
    17041 tcgatacgag ttataagatc cccgcgatga tcgaatatcg cgaccggctg gcaagagagt
    17101 ggcatctgaa tctcgtcgtc ggacagaaca aagaagccct cgcggccggg atgaatcaca
    17161 cgatggggcg agtcgtctgt tgcaccgcgc tgaagaccaa cggcctcaag cagctccttg
    17221 agcagaaggg ctataccggc gtgatcttgg gcgtgcgagc ggacgaggag ggcaccaggg
    17281 ccaaagaacg ctacttctcg ccccgcgata agcatggtga ttgggatttc cgcgatcaac
    17341 ccccggaact ctgggatcaa tacaagacga cgttccctcc gggcacgcac attcgcatcc
    17401 atccattgct ggactggacc gaaatcaaca tttgggaata catcaagctc gaaaacattc
    17461 cctttatcga cctctacctc gacaaggggg atgggacgcg ctatcggagt ttggggtgcg
    17521 ccccctgcac gacgccgatc aagtcgaccg ccaagagcgt ggacgatatc attgaagaac
    17581 tgcggcacac gaccgtcgcg gaacgatccg gccgtgcgca ggatgagggg cgagggatgg
    17641 agttgttgcg aaaagatggg tacatgtagc aacagaatgc tgaaacgagc atccagcttc
    17701 gttctcggct cgaaacaatc ctcaacgcac cccccacagg gtgcgtctct ggttgtttct
    17761 cgcctgcggc cttgctgggc tgcccgtttg agcattctga ggagtaagta tgaacacggc
    17821 gatcaataaa ccatccgaga atctcaacat cgtgatcgtc ggtcacgtgg accatggcaa
    17881 atccacgtta cttggacgtc tctatgccga tacaggatcg ctccctgacg gaaaactgga
    17941 aaaagtccaa gccatctgcc ggcagcaggg taaagaattc gagtacgcct tcctctttga
    18001 tgcgtttctt gaggagcagg agcaaggcat cacgatcgac accgcgcgca ctttttttat
    18061 ttggaaaggc cggcaataca tcatcatcga tgcgccgggg cataaagaat ttctcaagaa
    18121 catgatttcc ggtgcggcaa gagccgaagc ggcgctgttg ctgatcgacg ccctggaggg
    18181 cgtgaaagag cagtcgaaaa aacacggcta tctcctgtcg cttttgggcg tccgtcaatt
    18241 tgccgtggtg gtcaacaaga tggacctggt cggctaccgg caggatgtat ttgaaggaat
    18301 cgaaaaagaa tatcgggaat ttctggggca gttcggggca gtgccggaac gcattattcc
    18361 tgtcagcgcc aagctcggcg acaacattgc caaccgaagc caagcaatgc cctggtacaa
    18421 cggcccgacc gtgctggaca ctctcagcct cttcaagaag gagatggtgc gatcagaaca
    18481 gcccttgcga tttcccgtcc aggacgtcta caagttcgac gcccgtcgca tccttgccgg
    18541 tcgaattgtc gcgggacgac tgaagatcgg cgaccacctc gtcttttctc cgtcaaacaa
    18601 gcgcgccaat gtcaggtcgg tcgaagcgtt caatatcgaa ccgtctccta ccggagctga
    18661 agcaggtcaa tctgtcggta tcacgctcga tgagcaaatc ttcgttgaac ggggagagat
    18721 cgcaactcac caggagcatc tgccgttggt ctcgaccgcc ttccgtgcca acctgttttg
    18781 gttggggcgg cgctcgttag aaaaagggcg caagtatgtg cttcggattg gcaccaagga
    18841 agttgactgc gaggtggcgg cgatccatcg catcatcgat accatggatc tgaatcaaca
    18901 gcagggcagc agctccgtcg gcagaaatca ggttgcggag ctgaccatcc gaaccaaggc
    18961 tccgatggcg ttcgaccttt ccgcctcgtt tgaggcaacc ggccgattcg tgctggtcga
    19021 cgagtacgac atcgccggag gcgggatcgt gaccgagctg gttcacgacg atcaggaatt
    19081 tctccgcgaa gaagcacgtc gccgggactt tgcctgggtt aagggcgagg tggggatcga
    19141 agagcgggct caacagtatg gccatcgtgc agccatcgta ctcgtcaccg gcgggcgaca
    19201 taccggcaaa tctttcctgg ccaaaacgct tgaagccagg ctggttgcag acggccgaca
    19261 tgcctatttg ctggacgggg aaaacctccg gcggggtctg gacgccgacc tttctgaggg
    19321 ggagcggggg caagccacgg aaatggcccg gcgctatggc gaagtggcga gattgttggt
    19381 ggataccggc ttgatcgtcg tgtcgacgac caatcctttt ggcatgtcct acatcgaggc
    19441 cgcacaagca atcaggacgc tggtgcatcc gaccccggtg atttccgtcc atatgagcaa
    19501 ggtaccggaa gaagttccgc ctaataccga tattgtgttg tccggtcctt cggactttga
    19561 cgcggcaact cgccgtgttc tcgatgaact caaacggcgc ggagtgcttg cgcaggccat
    19621 cggcgcgaag ccgacattcc agtactcaat ttaggctctg aagccgttga tacctgcaca
    19681 tccgatccga ttttcccatt ttggccggtt tgactctccg gaaatctctg tgctaccgta
    19741 ttttcttcct acatccatgt gtttgtgagg caatggccca tggctcctga aaaggatctg
    19801 aagactattc tcggcaagct ccgctactcc gacgacgcca aggtcgttca acaaataacg
    19861 gagcaaatga agcaagtgca gacccgcatg gcggggatta agcacaagtt ggtcgtcatg
    19921 agcggcaagg gcggtgtcgg taagagtatg accaccgtta atcttgctct cgcgtttgcc
    19981 cgccagaatg cgcgcgttgg actgctcgat gtggacttga acggcccctg tgtgccccgc
    20041 atgttggggc tccatggaca gtccttgacc atgaccccgg agggggcgat tcccccgatt
    20101 ggcccattgg gcattaaggt cgcttcaatg gatttcttcc tgggtgccgc ttctccggtg
    20161 cgttggaaag gtccgatgga tgtcagcccg gtatggctcg ggctcatgga aatgaatgtt
    20221 attcgagagt tcttagccga tgtggcctgg ggtgaactgg actatctcct ggccgattta
    20281 ccgccagggg ctgctgccga caagccgcct gtcattgcgg gctttattcc cgatctggcc
    20341 ggagccattg tcgtgacgac tccttctgaa gtggcctccg atgtcgtgca aaagtctgtc
    20401 acctatgcac gcgacatggg gatcaaggtt ctgggcatcg tcgagaatat gagcgaatat
    20461 cgctgcccct cctgtggcga actgaacgag ctgttcgagg ggaataccga atccatgtgc
    20521 gaagcactcg atctccccct gttgggtcgc attccattcg atcggaagct tgcccggact
    20581 tttgacaagg gtgaaccctt gttggacgag acctatccga cgatccagcg ttatcaggaa
    20641 atcgccggac ggatcagaac gctgctggac tataagaaag tcctggccga acaactgtga
    20701 cgcttatctg agaggagccg ccatgaaatt cgtgtgcctc acctgcgaaa cctatatgaa
    20761 cttcgagaaa gttgagaagc cgggggaggg atcgctcggc gtcttttttg gctgcccgtc
    20821 ctgcggggcc aagttctcga tggtcacgaa tcccggtgaa acgcagatgg tcagctcgct
    20881 cggagttcaa ttagggggaa ggaccgtcgc ggctgaaccg ttcgagatga cccggggaac
    20941 gctcaaggat gaagctttgg ccggttccgg tcagatggcg gcctatttaa atgaaaagat
    21001 tcaaggcgga caaccggctg cggcatcgac tgcgccggca ccagcaaagg ccggagaaaa
    21061 gtccagcggg ggctgtccct tctccgccat ggtggcggaa atggggctga cttccagcgg
    21121 taagcctgcc acgagcgggg cgggtacttc tgaattcacc tggacagccg acgcaaagga
    21181 aaagctcgat cgacttccgg tgttcgtgaa accgatggtc caaagcagcg tcgaagccta
//
LOCUS       DATIFR010000199        36364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1477482, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000199 DATIFR010000000
VERSION     DATIFR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477482"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /gene="thiC"
                     /locus_tag="VLD60_11365"
     CDS             <1..1912
                     /gene="thiC"
                     /locus_tag="VLD60_11365"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSB45604.1"
                     /translation="GSPMSDSTSIVGNGKKTPSSTLTTAPFPASRKVFVTGLRPGVRV
                     PMREISLTSTKSMNGGVSTPNEPITVYDTSGPYTDPSVKIDIRSGLAAMRREWILSRG
                     DVEQLPDVTSQYGRMRATDPKLADLRFQHIRKPLRAKAGKNVTQIHYARKDLITPEME
                     FIAIRENQSREVAREFASRNGHGGGTAQHPGQPWGASIPKTITPEFVRDEVARGRAII
                     PANINHPESEPMIIGRNFLVKINSNIGNSAVASSIDEEVEKMIWSIRWGADTVMDLST
                     GKNIHETREWIIRNSPVPIGTVPIYQALEKVGGKAEELTWEIFRDTLIEQAEQGVDYF
                     TIHAGVRLAYIPMTARRMTGIVSRGGSIHAKWCLAHHEENFAYTHFEEICEIMKAYDV
                     SFSLGDGLRPGSIADANDEAQFAELETLGELTKTAWKHDVQVMIEGPGHVPMHMIQVN
                     MEKQIKECHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGL
                     PDREDVKAGVITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWADQFNLSLDPDTA
                     KQFHDETLPDDAAKVSHFCSMCGPHFCSMKITQDVRDYAASKKVTEQQAIQIGMKEKA
                     DEFKKTGAELYR"
     gene            1924..2724
                     /locus_tag="VLD60_11370"
     CDS             1924..2724
                     /locus_tag="VLD60_11370"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HSB45605.1"
                     /translation="MGRPKPAPLRERDITRQIAREYYKEFDQLIESDVIIVGAGPSGL
                     ICAHDLADMGFRTVVVEQSLALGGGFWHGGYLMNKATICAPAHKILEEIGVPCKKVKD
                     CEGMYIVDPPHATGALVAAAYRGGARVLNLTRVVDLILRRDGVLEGVVVNNTTAEMAG
                     HDVIHVDPIALESKIVVDATGHDAVVVNLLHKRNLYTEVPGNGAMWVSRSEEDVMDHT
                     GEVYPNCFVIGLAVAAVHGTPRMGPAFGSMLLSGRYGAELIKKKLKNE"
     gene            2728..3243
                     /locus_tag="VLD60_11375"
     CDS             2728..3243
                     /locus_tag="VLD60_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45606.1"
                     /translation="MDVQDFLVQGEGREEDKREAWQLFQQAYERQMKGELEEAVSLYR
                     QSLATHPTAEAHTFLGWTYSFMGRLNDAIEECRRAIERDPDFGNPYNDIGAYLIEKGE
                     LDEAIPWFQKAMQAKRYESPAFPHLNLGRVYEKQGNWTEAIASYKQALALNPDYSLAK
                     KSLGRLISSLN"
     gene            3356..3784
                     /locus_tag="VLD60_11380"
     CDS             3356..3784
                     /locus_tag="VLD60_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSB45607.1"
                     /translation="MSQTNQKTILVAVTDIFFYTKVRDALLPSGYKLERARTQEDIAG
                     KASALNLSAVILNMNDDTVNAFKALETLKADPQFKTIPILAFANHEEVDTWNRAKALG
                     VTKIVSRNEFSARTKDLMEEMIATSCLQSAVSPELKADSF"
     gene            3796..4890
                     /locus_tag="VLD60_11385"
     CDS             3796..4890
                     /locus_tag="VLD60_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HSB45608.1"
                     /translation="MKQSRLHNHHRTLGAIFEDASGWTMPAHYGDPVAEYRAVRQAVG
                     VTDLSHRGKVRVTGDDRVKWLQSIISNDILPLQPGQGRYSSFLTHKGKMLTYFRVYMQ
                     ADSVILEDAGEIGDATYTALRKFLLYGTKAKMENCAENWGLLLVSGPKAAVVMNAAFG
                     TDVEDLKPLSFISAAIGGQTALIIRTEETGESDYEVLIPADGLVAAWERLMDAGAQHG
                     IKPFGIQAHESLRMEAGIPKAGPDLNEEIVPPEANLEDKAFSLSKGCYPGQEVVARMD
                     TYGNVRRKLVGLVLKDSTVPPHGAKLFSGDREVGWVSGATHSPHLNNAVIAFGFPLRD
                     FSAPDTALTVEVEGKRLAATVKPLPFYSRS"
     gene            complement(4896..5378)
                     /locus_tag="VLD60_11390"
     CDS             complement(4896..5378)
                     /locus_tag="VLD60_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45609.1"
                     /translation="MAGLMSAEEIFEKTQAAAVAATSPDEKALQIDYAALKDKIRAAL
                     GDRKVALCHINKFLPEGYEDQGRFNVLLLTAGNVVFDIVIGDSYFRYDIVSVGQLDKV
                     QVIDAMWDNKEKRREEPFLSLRLMHGEEAHLLLALEDEERTSLLAFARAVSAARNPEK
                     "
     gene            complement(5402..5650)
                     /locus_tag="VLD60_11395"
     CDS             complement(5402..5650)
                     /locus_tag="VLD60_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45610.1"
                     /translation="MLLQIISVVGALMVLTAYGLNLGGVWRELHAGYLTLNIVGALLL
                     GIVAIVDQRAGFILLEIAWVGLGFVGVARAVRARQNQQ"
     gene            complement(5750..7108)
                     /locus_tag="VLD60_11400"
     CDS             complement(5750..7108)
                     /locus_tag="VLD60_11400"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSB45611.1"
                     /translation="MPDVLPQGIALGDVTAHGALLWVRTEGPALVQVEYAPPSVWEKA
                     SKMATAVAPVAKSARLATTVDTDFTVTIPLEGLSPATRYRFYLFVGRASDTKNHIDAA
                     LAAKWEFTALPDAATSVPVTFAWSGDLGGQQRCRQGAGGYPIFDMMQKQGLDFFVFLG
                     DTIYSDDTCPSPPNEPGADFKASTLSEYRARHRYQRGAQSLRRFLETVPVYVIWDDHE
                     VRNNFAGPYDEQMPAGRQALREYWPIASPSEDPHRMYRALRYGADLELFILDTRQYRS
                     KNADQDGPAKTMLGAQQLKWLLDGLRGSNATWKVIVTSVPLSIPKGGGASVPGYDGWA
                     GGSDGTGFERERQVIVDAIMLNKIKNVVFLGGDVHWVQANSYDSNGDGIPDFHEYIAG
                     PLSASPGRLTPPSPALHPKNLINEAGYYNFGLVRVTPNSFDVTIIDDTGKTRFSHRLA
                     AR"
     gene            7544..7780
                     /locus_tag="VLD60_11405"
     CDS             7544..7780
                     /locus_tag="VLD60_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45612.1"
                     /translation="MNNLNCLIQGCQGGVYSPAGTQNLCKEHFLSFLTWRRRRGPQMF
                     TTYAGMTMEQRDPIVAEWAKTIRVEEVSTAPTKT"
     gene            complement(7850..8497)
                     /locus_tag="VLD60_11410"
     CDS             complement(7850..8497)
                     /locus_tag="VLD60_11410"
                     /inference="COORDINATES: protein motif:HMM:NF037888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45613.1"
                     /translation="MGLIGTPVDGKNIGLAKVLYGLRQDSDVYCLISVNAASIKERGG
                     GVFFGHIQMRAIESIVLCICKIFEDEKQYELESIRGVIRYLMTQPASCLDESKVKEFL
                     LNYGCVSSADSLAGKLQNTVEVFRTKYKDELERFKTFRDKKAAHSEYGVIIDSLPSYD
                     VMEKLFLFGADFYKLVSAAFVGVGPDNLETNRPVKVNLKNLLERLGLQSIQTDMK"
     gene            complement(8760..9152)
                     /locus_tag="VLD60_11415"
     CDS             complement(8760..9152)
                     /locus_tag="VLD60_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB45614.1"
                     /translation="MIEQRIEEVTKANQRFYEAFETLDIAKMDEVWAHLEYVTCVHPG
                     WTLRSDWPNVRDSWVLIFNNTFSMKFELTEVMVQVAGDMAWVICIENITTQQSDEAQQ
                     ARILATNLFERIGDEWLMIHHHGSAVMG"
     gene            complement(9222..10127)
                     /locus_tag="VLD60_11420"
     CDS             complement(9222..10127)
                     /locus_tag="VLD60_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSB45615.1"
                     /translation="MNQLTDIAASGAIVAAAVLWGGSIVAQKLALSGFSAVEASVLRD
                     IGGLAILLSIWWWQEGRMVSLTRPDLRTLALLGLGVVGNHLLILIGLKYVSGAVAGVI
                     IGSAPVVTALLSALMIRDVPLRAVWAGGLLSFVGVGLVSVAGFQAAGEQPLLGGTLVF
                     LGVVSWALYSIGSRGVMDRLSALTVNWTTLLVATVLQIPLLWTDQKMVQSGVASVAAK
                     DWLALGYLVVFATAVAQQAWLFGVKGIGPSRASVLGNLTPVAAVVLSAMILKESVGLV
                     EIIGIVLILSGVWVVHRQTSQLAGT"
     gene            10335..11852
                     /locus_tag="VLD60_11425"
     CDS             10335..11852
                     /locus_tag="VLD60_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB45616.1"
                     /translation="MKPAEKEWLVDGTMVLLTTALFVLDVYTPLSFANHFLYATVILV
                     STASRVSFMPWLVAGVTSALTVIGGFISPPFPDLPMWIPIGNRAFTIMIVWILAWFAV
                     KRRQAESALQQSNECLEEKVIQRTRELAGVNQALVSEVTERMQTEQAYRQSEGRLAGI
                     LDIAEDAIIVIEQDRSIALFNQGASKLFGYDSADVLGQSIDRLLPERFRLDHARHIQG
                     FAQSQEPARRMAERQEVFGLRKDGTEFPAEASISKLTVGGKTTFTVILRDITERLQTE
                     HQLHSLATQLMTAQEEERRRISRELHDDINQRLALLAIEMGNLENEPALPADQAKRSF
                     QSLTLRLAAISDDVRRMAYRFHSSILDDLGLSAALQRLTDEFSTRTGIKTVVVQEDLT
                     EPLPREIASCLYRIVQESLVNVTKHAQASRVELELTCDGQEITLAIHDSGAGFDLNQI
                     RAQPHGLGLVNMRERVRSVHGRFEIRSQPGHGTHISVLIPLPGTHDEKTTSPLGG"
     gene            11821..12465
                     /locus_tag="VLD60_11430"
     CDS             11821..12465
                     /locus_tag="VLD60_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45617.1"
                     /translation="MKKPRVLLADDHTLVLEGFKKLLEEHCELVGTAEDGRTLVDLAA
                     QTHPDLVLLDISMPLLNGIDAAKKLKKLLPDVKLIFVTMHADTAYINEAFKAGASGYL
                     LKRSAAKELVQAVQSVMNNSYYVTPLITKDVISSFLMPGQTRVPAVDDLTARQREILQ
                     LVAEGFSAKEIAGQLKVSHRTVEFHKAKIMELLDLHTTADLVKYAVAHGLVSAS"
     gene            12628..13089
                     /locus_tag="VLD60_11435"
     CDS             12628..13089
                     /locus_tag="VLD60_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45618.1"
                     /translation="MRRLRIVLADDHPQILSLLQVLLEPDYKVVGTAEDGQSLIAAAQ
                     ALRPDVVITDIDMPFLNGIEATRELHKLLPDCRVIVHSSHSEPEIIAAALAAGASGYL
                     IKGSSQSLISSIRSVIRHVSAHDDAPNVNRVPEHAIPRESRARQESSWGNL"
     gene            13201..13452
                     /locus_tag="VLD60_11440"
     CDS             13201..13452
                     /locus_tag="VLD60_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45619.1"
                     /translation="MQCPRCRNLVVEEVFVDYESDGAMSFVGFRCVICGNILDETIQR
                     HRAEHGSSLCGNRRRHPLSMRPERETREARSDESKEYHT"
     gene            13506..13913
                     /locus_tag="VLD60_11445"
     CDS             13506..13913
                     /locus_tag="VLD60_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45620.1"
                     /translation="MEKAPVRRRILVIDHDDDVRAFLCDRLSALGFEVAAENNGISGL
                     SRVADGRDAAPFHGVLVELQMPVLDGLSVLREIHARFPTIPVVVMADSIHVGKLRQAM
                     TAGAKEYLVKPFDPELFRRKCVNVFLDGKELAR"
     gene            14459..14932
                     /locus_tag="VLD60_11450"
     CDS             14459..14932
                     /locus_tag="VLD60_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45621.1"
                     /translation="MVTQQAMWIKMWERQTRARIGLRGRLAVASLLSILLSGILSCTA
                     GPHAGGSGAVRYIQIRHDVTPSTLYAQVGDEIRWHNLSQDPVRVGILDNKWRDHVACE
                     KGFTRFGAMEDLVTIQPQEYVSLCFSKAATVRYNVWLHAQNLKGAMSPTAAIRID"
     gene            15152..16438
                     /locus_tag="VLD60_11455"
     CDS             15152..16438
                     /locus_tag="VLD60_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB45622.1"
                     /translation="MALRFRFIFTSLAIVWQMGGLCGTLAASAESGPQAYQLNAIVEL
                     ALARNPLMSSAEGHIDQQRGQQTAAGAYPNPTVTGNIGYGEIRDAGRADIQSSLDRRS
                     LTEYNVTVGQPLEWPSLRAARQQVAEGGLATATASLSETRLNLTTQVKVAFYDLLLAQ
                     QDAALARQNLDIVESVARIVKARVKSGEGPQFELIKAEVEVLKAKQQLTRADNVVRVN
                     RVVVDTLTGGALGPSYVVQGDFAGMSKELHVEELMARMAIRHPTIQRLLKSVEQSDWK
                     IEYERQSRVPSVTVNGSYWREIGREAVQGGLSVPMPLWYRRQGEIASSLGTKRREEAE
                     LLRARNELARAVHQHYQDARTTADLIEVFDKGLLKQAQEALRLAQFSFQQGASSLLEV
                     FDAQRVQRQIQMDYAQVRYELSVSLARLERAVGGTL"
     gene            16435..17682
                     /locus_tag="VLD60_11460"
     CDS             16435..17682
                     /locus_tag="VLD60_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB45623.1"
                     /translation="MNPVSLSRNVSCGREERAKRTPSPRTFAVVYGLLAALAAIGSGC
                     DSNPSDAVATKPRAVASAPGVVLLPPEESSRIGLVVQPATRSDFRTYREFPAILQPNE
                     RAMADITTLVRGRVVDVYADLGQQVEANALLAILYSSELGLAQSAYLKSRAKLHVAEQ
                     AYARAKFLLKEMVIGEAEAQRRQAELLSMQAEAHEARDHLKLLGMTDDEFRRLDKSRD
                     IRSHVPIVAPFAGRIIGRNLTRGEVVETTEKLFVVADLSEVWVRANIPEKDIPFVHSV
                     HASGGTQADVRVNVYPRELFKGTITYVGDVLDPATRTMQLRLELPNPERRLKPEMFAT
                     IRLYSESQPDRLAVPESALQRDQDRTFVFVQRNVNEYEVREVQIGESNGTHTTILDGL
                     SEGDPVVTHGAFVLKSELLKKQV"
     gene            17682..20921
                     /locus_tag="VLD60_11465"
     CDS             17682..20921
                     /locus_tag="VLD60_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSB45624.1"
                     /translation="MVSRLLDISLRQRMLIVIFSIMIGAGGIYAFRTIPIDAFPDVTS
                     VLVQVVTKAPGLSPAEVERLVTYPIELQLTGVPSLTEMRSLTKVGLSLVTIVFDDAMD
                     INLARQLVLERLIEVKELLPPGAEPMLVPNSTGLGEVFQYYLEGPQGPVPDEAVQHQN
                     LIDHRTIQDWIIRPRLKSTPEVIDVNSMGGYVKQYQVLVEPVMLRKYNLTLREIFDAV
                     ANNNANAGGNILEKHAEKYIVRGVGLIRTLEDIERIVVKEVGGTPVFVSDVAQVLIDH
                     AVRHGATVLNGEREVVSGIVLMLRGGNARDVVEGIKARIDDIHRQHLLPDGLRIVPFY
                     DRIELITAALTTVYKSLAEGVVLVVVVLFLFLGNIRSALIVVSTLVLAPLATFIVMGQ
                     VGLTANLMSLGGLAIAIGMIVDGSVVVVENVYRHLSTHSAVAVPRRDLIMTAVKEVGQ
                     PVVFGILIIILVFLPVLSLHGMEGKMFKPLAYTIMIALLVSLVLSLTLSPVLCSLMLK
                     RGSEEDPWIVRWAKRLYAPSLHWALGNRNVVLAVAFGSLLCSLALFPFLGSEFIPILN
                     EGSVAPQTIRLPSVSLPASIEIEKRMQQAIMEFPEVEMVVSKIGRTELGNDPQEPNES
                     DPVVRLRPLEQWTTARTMPELMQKFRERLSRIPGATFLISQPIQQRVDELISGVRTEA
                     TVKLFGDDLEILRSKADEIAGVLGTVRGVRDIKVEQLYGQPYLTIDIDRSKIARHGIN
                     VADVREIITTAIGGQAATRVYEGQQRFDLFVRFPEQYRDSPETISNILVASGNGALIP
                     LADLGTVRLEEGPGRISREQLQRYVSIGFNTLGRDIGSLVAEAQQKIASQVSLPTGYT
                     VAWGGSFENMERAMAKLRIIVPITIGLIFLLLYSTFDSLRQATLIILNLPFALIGGVV
                     ALWLTGEYLSVPASIGFINLFGVAVLNGIVLVSCMNKLRQDGCSLDEAVFTGSLLRLR
                     PVLMTALVALLGLVPLAFARGIGSEVQRPLAIVVIGGLVSSTLLTLVVLPVLYRWVEG
                     RSGARPETDRGPMDLGPSHEADRQTDQMDGQRPSHTHIRTPPPRPLRQS"
     gene            20930..22078
                     /locus_tag="VLD60_11470"
     CDS             20930..22078
                     /locus_tag="VLD60_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HSB45625.1"
                     /translation="MDITTDLGTQTKLRASHEWPLAVSVITAALFLSFGSQWLGDLSH
                     PAWFACMLMWLFTVILLSAFAVIRHAEVIAEQLGEPIGTLVLTLSVTGIEVMMIAAVM
                     HGGKGNATLARDAMFAVVMIVLNGMVGLSLLLGGLRYHEQTYNLQGANAFLAVIVPLA
                     VFGLVLPNFTVSSPGPTLSPLQTAFLIIMSIGLYGVFLAIQIWSHRSYFVLPEIEQGL
                     MGAHRLPGQHERGGLSVRYPIGMLLAYLLPLVVLSKQLAIPINYGMHELQAPPALGGL
                     LVAILVLSPESLGAARAALANQLQRSVNILLGSVLATISLTIPAVLLIGFTTSQIIIL
                     GLDAADMTILLLTLGLSTLTFASARTNVLLGAVHLLLFLAYVMLIFEK"
     gene            22327..22572
                     /locus_tag="VLD60_11475"
     CDS             22327..22572
                     /locus_tag="VLD60_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45626.1"
                     /translation="MTIATMADRVRGALETRQTDYPMDEIVALCPDLTWSQVFKAVDH
                     LSRTGQVRLRINRRRTYMVKALRLPMPIHPCADSIPK"
     gene            23538..23894
                     /locus_tag="VLD60_11480"
     CDS             23538..23894
                     /locus_tag="VLD60_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45627.1"
                     /translation="MNGRIETKYVALVLLFIFCQVIGTMCALPDLSVAEEIATLVEEG
                     MVCPMDGTIMCPPSITSSPERQIMHVLVADFDHATILLGSAAGLPVRSAPSLWSWSSS
                     SSIVPISISSPSVLRI"
     gene            23986..25329
                     /locus_tag="VLD60_11485"
     CDS             23986..25329
                     /locus_tag="VLD60_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB45628.1"
                     /translation="MKPSIVPVQGYIIPLVVFSAVVLSPWSVRGAEDRGIVVPDGTAH
                     TSHSELRIDLPGLIGELERANPEIKAARQRWEASKAVVPQVQTLPDPRLQLGYQRMPM
                     VPPVVEGAMYGFGQEIPFPGKLGLKGEVAQREAERLEQEFNATRLRLIATLKEAYFNL
                     HFVHKSIEIVERNKVLLMQFEKTAKARYSVGQAAQQDVFRAQVEISRVLDRLAVLDQQ
                     KESLHAAINRLLNRPPAGPLGAPAEIQTTILTIPLQDLSRRADEFSPALLATAKSIDR
                     SERSVSLAKRQYYPDFDITALGLRNDKINDNGYQVMVGIKIPLFYETKQKQGVREALA
                     GLEGAKEDFATTRQDLLFQVKDGFVQAQRAERLITILRDAIIPQATLALQAAQASYTV
                     GKVDFLTLLNSLLILQDSQLELHGEMVNHEKALARLEAVTGGPLSRPLSERKPGS"
     gene            25326..26579
                     /locus_tag="VLD60_11490"
     CDS             25326..26579
                     /locus_tag="VLD60_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB45629.1"
                     /translation="MTRQRLIGMAALLILAAVAVVWLTSRPLMLPPADSRQDDMAGMD
                     MPGMGKAQQQKSAPAEKAVGPEIPLESEGVPGIVTIPPERLQTIGVKYEQVARRPLEK
                     VIRTVGRVVMDERKLAKVTIKFHGWIEQLFVSATGDHVKKGQRLFTIYSPDLVATQEE
                     YLLALQSRKQLGESEIPEVAAGSKDLLEATRHRLHLWDIGEEHIRELERTKQVTKTLP
                     IHSPISGTVIRKEVLQGTHVEPGEELYTIADLSHIWILADIYEYELSFVKIGQQAAVT
                     LSYDPSTVLTGQVGFVYPTLDPKTRTAKVRFELDNADEKLKPDMYANVELRVNLGTRL
                     AIPQEAVIESGEKQVVFLHLGGGKLEPRLIKTGVKTGEWSEVLTGLKEGDHIVTSANF
                     LIDSESRLKSVVEGMGGMSGMKSKD"
     gene            26587..29715
                     /locus_tag="VLD60_11495"
     CDS             26587..29715
                     /locus_tag="VLD60_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSB45630.1"
                     /translation="MVERIIEFSARNRFIVFLFVFALSSAGLWATRHTPVDALPDLSD
                     TQVIVYTTWAGRSPTLVEDQVTYPIVTALLSAPNVTVVRGFSDFGYSYVYVLFKDGTD
                     IYWARSRALEYLNQLAGRLPEGVTPQLGPDATGVGWVFEYALVDETGQHDLAALRSFQ
                     DWYLRYWLRGVEGVAEVASVGGFVRQYQVNLDPTKVLAYKLSLSAIIETIRQSNNDVG
                     GRVVEFTGIEYVIRGRGYIQNTTDIEKIAVAVNENGTPILLRDVATVRLGPDMRRGLV
                     DLNGQGEVVGGIVVMRFGQNALEVIDRIKAKLKELEPSMPRGVKVVTTYDRSDLIRES
                     IGTVKENLIEELLIVSAMLVGFLLHLRSALMPILTLPIAVLFSFLPIWYFGVGMNIMS
                     IGGIIVAIGDMVDASIVMVDNAHRRLEEWERGGRVGDRTQVLIDSAKEVGPSIFASLL
                     VIAIAFMPVFTLEAQEGRLFRPLAVTKNLAIAMSAVLAITLIPALLDFLVRGRIISEQ
                     RHPISRTLQRLYAPVLRFALQFRWAVVVGAVLLTLLTVPPFMRLGSEFMPPLYEGTIL
                     YMPTTLPGLPVTEAGRLLQIMDQKLRAFPEVAWVFGKAGRAETSTDSAPFGMMEVVVE
                     LNPKDQWRPGLSYEGLVDEMDRALQLPGVTNAWTMPIKARIDMLTTGVRTPVGIKIFG
                     PDLTEIGKIGEHLEMVLKEVPGTRSVYAERVFGGYYLDFVINRDEIARYGLKVMDVGR
                     IIESAIGGENIATTIEGRERYPINVRYLRELRDEPEKLRRVLVDTPTGAQVPLAQLAT
                     LRFVNGPPMIRDENGMLAGYVFVDMVGRDMGSYVDDLKRVVQEKVSLPTGYTITWSGQ
                     YENMQRVRERLTIFIPLTITIIFVLYYFTFRSVAESLMVLLGVPLSMVGGIWSLALLG
                     YNMSIAVWVGLIALAGVGAETSAVMLAYLDEACARRQKAGRLRTLSDLIETVQEGALV
                     RIRAVTMTSLANILGLLPVMWATGTGADVMKRLAAPMVGGVFTAMLLTLVVIPALYVF
                     WRWHSDVKQSGI"
     gene            29867..30046
                     /locus_tag="VLD60_11500"
     CDS             29867..30046
                     /locus_tag="VLD60_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSB45631.1"
                     /translation="MAKDPVCAMEVDDQSAKWTSTYEGKRYSFCSAGCKDSFDKDPDR
                     YLLDRDLLGRKIKPQ"
     gene            complement(30273..30917)
                     /locus_tag="VLD60_11505"
     CDS             complement(30273..30917)
                     /locus_tag="VLD60_11505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45632.1"
                     /translation="MKSLVTPEIEAYAEAHSMAESAICRALREETYRRMEFPQMLVGP
                     LEGAFLKMMAQVVGAKRVLEVGMFTGYSALCFAEAVPEDGTIITCEVDEESATLAGKY
                     FARSPVGKKVEVRMGPALETMQNLDGPFDLIFIDADKQNYLNYFRRALELLAPNGIIL
                     IDNVLWGGEVLKHPPPDQRTAAIQELNKAVSSDGKVSAVLVTIRDGILVVRKKG"
     gene            complement(31040..31483)
                     /locus_tag="VLD60_11510"
     CDS             complement(31040..31483)
                     /locus_tag="VLD60_11510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45633.1"
                     /translation="MKALVSAEIEAYAEAHSVPESEVCRALREETCRVMEYPQMLVGP
                     LEGAFLKITAQLVQAKHVLEVGMFTGYSALCFAEAIPEDGRVTTCEIDEASAVLARRY
                     FARSTRGTKMSIRMGPALDTMKDLTGPVDLIFIDEVLSPKLRMLS"
     gene            31599..33131
                     /locus_tag="VLD60_11515"
     CDS             31599..33131
                     /locus_tag="VLD60_11515"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HSB45634.1"
                     /translation="MKTLKTLEPLATRLLEIQRINSAASVLSWDQETYMPAGGGSARA
                     EQIATLQGIAHKKLVSPDTERLLGNWLDLGTGQPLEQDGELWDESSRALLREVWRDYS
                     RAKKLPSDFVMTMSRECSLAQQVWIEAKDEHSFKRFLPNLCTVLSLKRREADYLGYQG
                     SPYNALLDLYEPGSTIAALRPLFAQLKARLVPLLKKIQNSSVQIDDGFLYHAYDQARQ
                     LEFGRLVLIAMGYDFERGRLDLSAHPFTTSFHPTDVRVTTRVQEHDLPSCLFSCIHEG
                     GHGLYDQGLDPLHYGTPLGESVSLGIHESQSRMWENCVGRSRSFWRFFFPILQANFHH
                     QLRDVDLEQFYAVVNRVKPSLIRVEADELTYNLHIMLRFEIEQDLIEGNTKPEELPEL
                     WNQKMKEYLGVVPSHDGEGVLQDVHWSMGAFGYFPTYTLGNLYAVQFYDQAKLEIPHI
                     EDEIAAGQLMVLRRWLGQKIHRWGRMFTPDHLAQRVTGVSLSPEPFLRYAERKYGELY
                     RL"
     gene            complement(33182..33745)
                     /locus_tag="VLD60_11520"
     CDS             complement(33182..33745)
                     /locus_tag="VLD60_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45635.1"
                     /translation="MRKPHQITVDRALLLYLLHQAEPHGLLSDVKLQQLCFLCELQMF
                     GKGFKGFHFEFFRFAYGAFSKDLDNDLTSLRRKERVENFTLSDQAREEVLPLIEKGVA
                     GVEANEKVIEILKAVVATYGPHDSGAITNSVEAVELSTPQDPEFKIPIRDIVFHTTLL
                     VPHRIEVQTEFTFPPALLMKLNQAMGY"
     gene            33880..34317
                     /locus_tag="VLD60_11525"
     CDS             33880..34317
                     /locus_tag="VLD60_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HSB45636.1"
                     /translation="MASGKHVKEMESLKEHLAKHHLKFTRQRELILSAFLRQEHITAE
                     AMYHQLAKTDPHLGLATIYRTLNLFCDAGIAQARHFGSQTQYDNVSHKGHHDHLICTG
                     CGKIVEFENCEIEKLQEEVATRNGFTIQTHRLELYGLCSRCRH"
     gene            34385..35437
                     /locus_tag="VLD60_11530"
     CDS             34385..35437
                     /locus_tag="VLD60_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSB45637.1"
                     /translation="MLFRSTWITPRRTTVGLLTLLFTIGLILAGLVQASPAADKITVY
                     SGRAERLIKPVFDDFTVKTGIQVELLSSGTTELVNRLKAEGDRTAADLLITNDAGSLE
                     LARTAGILRPLSMREIDRAIPPQFRAPDNAWVGLSGRFWIIVYNTSMVKPDQLKSLLD
                     LADPKWKDKIAIPNSGSEYLQAGVSVIRATHGDAKTKQFLQGLKDNAGGQVYQKSSQI
                     VDAVAKGQVALGIVNHYYVYRHLATQPAAPIAVFMPDQQDGGMGAIMNVAGIGIVKST
                     KHLESAKLLVEFLVAQAGQKLFAELDKEYPLHPEVKADPALVERKSFRAAQVPLTKLA
                     ELREPTLLLIEQVGLR"
     gene            35454..>36364
                     /locus_tag="VLD60_11535"
     CDS             35454..>36364
                     /locus_tag="VLD60_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSB45638.1"
                     /translation="MITIRQQLSSPLQLIAVASAGIILLPLGYVTTQALSADPAVWSR
                     LWATRIPELLSNTIWLAATVACLTLILGVSTAWLVTRLDFPGRRLWEGALVLPLAMPT
                     YVLAYVYSYLLGFGGPVEHLWQIVAGPQARIFSPHSYLGATLVMTLDTFPFVYLLSRS
                     ALLSMNVSFEEVSRASGVSRVQTLWRVTLPLIRPSIAAGLALVILYVVSDFGAVSLLR
                     YQTLTYAVFQQMTGRSDNTAASILSLLLVGLALIFLVTERWFRHRSRFYQTTGRYRTP
                     QRHRYGWLGSLLVTGYLGLIVGAAFALP"
BASE COUNT         7970 a        10690 c        10254 g         7450 t
ORIGIN      
        1 aggatctcct atgagcgact ctaccagtat agttggcaac gggaaaaaga cgccgtcctc
       61 gaccttgacg accgctccct ttcccgcctc ccgcaaggtc tttgtgacag gcctgcgacc
      121 cggcgtacga gtgccgatgc gagagatcag cctgacgtcg actaaatcga tgaacggcgg
      181 cgtctcgact cccaatgaac cgattacggt ctacgacact tcaggcccgt acacagaccc
      241 ctccgtgaag atcgatatcc ggtcgggact ggccgccatg cgccgcgaat ggatcctctc
      301 gcgcggcgac gtcgagcagc tccccgacgt gacctcgcag tatgggcgga tgcgcgcgac
      361 cgatccgaaa ctcgccgact tgcgctttca acatattcgc aagccgctcc gcgccaaggc
      421 cgggaagaac gtcacccaga ttcactatgc ccgcaaagac ctcatcacgc ctgagatgga
      481 attcattgcg atcagagaga accaatcccg cgaggtggca cgggaatttg cttcccgaaa
      541 cggacatggc ggcggcaccg cgcagcatcc ggggcagccg tggggcgcca gcatccccaa
      601 gaccatcacg ccggagttcg ttcgcgatga agtggcacgg ggcagggcca tcatccccgc
      661 caacatcaac cacccggaaa gcgagccaat gattatcggc cgcaattttc tggtcaaaat
      721 caattccaac atcggcaact ccgccgtggc ctcatcgatc gacgaagaag tcgaaaagat
      781 gatttggtcg atccgctggg gcgccgacac cgtgatggat ttgtcgaccg gcaagaacat
      841 tcatgagacc agagaatgga ttatccggaa ttcgccggtg ccgatcggca cggtgcccat
      901 ctatcaagcg ctcgagaaag tgggcggaaa agccgaagag ctgacgtggg agattttccg
      961 cgacaccttg atcgaacagg ccgagcaagg cgtcgattac ttcaccatcc atgcgggcgt
     1021 gcggctcgct tacatcccca tgacggccag acgcatgacc ggtatcgtct cgcgaggcgg
     1081 ctcgatccac gccaaatggt gcctggcgca tcacgaggag aactttgcat atacgcactt
     1141 cgaggaaatc tgcgagatca tgaaagcgta tgatgtttcg ttcagcctgg gagacggatt
     1201 gcgacccggc tccatcgccg acgcgaacga cgaagcccag ttcgccgaac tcgaaacgct
     1261 gggcgaactc accaagaccg cctggaaaca cgacgtgcag gtcatgatcg aaggtccggg
     1321 ccacgtcccg atgcacatga tccaggtcaa catggagaag cagatcaagg aatgccatga
     1381 ggcgccgttc tatacgctcg gccccctcac gaccgatatc gcgccgggct acgaccacat
     1441 cacctcgggc atcggggccg ccatgatcgg ctggtacggc tgcgctatgc tctgttacgt
     1501 gaccccgaag gaacacctgg gcttaccgga tcgggaagac gtcaaagccg gcgtcattac
     1561 ctacaagatc gccgcgcatg ccgccgacct cgcgaaaggc catcccgggg cgcagattcg
     1621 cgacaatgcg ctctccaaag cccgcttcga gttccggtgg gctgatcaat ttaacctgtc
     1681 gctcgatccg gacaccgcga aacagttcca cgacgagacg ttgccggacg acgccgcgaa
     1741 ggtctcgcat ttctgttcga tgtgcggccc gcacttctgc tcgatgaaga tcacgcaaga
     1801 cgtgcgcgac tatgccgcga gcaaaaaagt gaccgaacag caggcgattc agatcgggat
     1861 gaaagagaag gcggatgaat tcaagaagac cggagcggaa ctctaccggt aggagcgacc
     1921 attatgggaa gaccaaaacc agcgccgttg cgcgaacgcg acatcaccag acagatcgcg
     1981 cgcgaatact acaaagaatt cgatcaactc attgagagcg acgtcatcat cgtcggcgcg
     2041 gggccgtccg gcctgatttg cgcgcacgat ctggcggaca tgggctttcg caccgtcgtt
     2101 gtcgaacaga gcctcgccct cggcgggggg ttctggcacg gcggatacct catgaacaaa
     2161 gcgacgatct gcgctcccgc acataagatc ctcgaagaaa tcggggtgcc ctgcaagaaa
     2221 gtcaaggact gcgaggggat gtacattgtc gaccctcccc atgccacggg cgccctcgtt
     2281 gcggcggcct atcgtggcgg cgcccgggtc ctgaacctca cacgcgtcgt cgatctcatc
     2341 ctccgccgag acggggtgct ggaaggtgtg gtcgtcaata acaccacggc cgaaatggcc
     2401 ggacacgatg ttatccacgt cgatccgatc gcgctggaaa gcaagatcgt cgtcgatgcc
     2461 accggtcacg atgccgtggt ggtcaattta ttgcacaagc gcaatctcta caccgaggtg
     2521 cctggaaacg gcgccatgtg ggtctcacgc tcggaggaag atgtgatgga ccacaccggc
     2581 gaggtctatc ccaattgctt cgtgatcgga ttggccgtag ctgccgtcca tggcacacca
     2641 cgcatgggcc cggctttcgg ctcgatgttg ctatccggcc ggtacggagc ggaactcatc
     2701 aagaagaaac tcaagaacga gtagtctatg gatgtgcaag atttcttagt acagggtgaa
     2761 ggaagagaag aggataaacg agaggcctgg cagctctttc aacaggcgta cgaacgccag
     2821 atgaagggcg aattggaaga ggccgtcagc ctgtatcggc agtcgcttgc cacgcatccg
     2881 accgcggaag cccatacctt tcttggctgg acctacagct tcatgggacg gctcaatgac
     2941 gccatcgaag aatgccggcg cgctatcgag cgggatccgg atttcggaaa cccctataac
     3001 gacatcggtg cctatctcat tgagaagggc gagctcgacg aggcgattcc ctggtttcag
     3061 aaagccatgc aggcaaagcg gtacgaaagc ccggcctttc cacatctgaa tctcggacgg
     3121 gtctatgaga agcagggcaa ttggactgaa gccatcgcct cctacaaaca ggctttggcc
     3181 ctcaaccccg actacagcct ggcgaagaaa tcgcttggtc gcctgatcag ctcactcaac
     3241 taaactagtt tgttttgttt gtctgcttta tctcgtgtac atggttcgga ggacgatctc
     3301 ctcgcatatc aaacgccgcg tcaaccagac gaaccacaca aactaaataa accaaatgag
     3361 ccagacaaac cagaaaacca tcctcgtggc ggtgaccgac atcttctttt acaccaaggt
     3421 gcgcgacgct ctgctcccaa gcggatacaa actcgagcgg gcacgcacgc aagaagacat
     3481 agcaggaaaa gcctcggcgt taaacctgtc tgccgtcatt ttgaacatga acgacgacac
     3541 ggtgaacgca tttaaagcgc tcgaaacact caaggccgat ccgcaattca agactattcc
     3601 catccttgcc ttcgccaatc acgaagaagt cgacacctgg aaccgggcta aggcgctcgg
     3661 cgtaaccaaa atcgtgtccc gcaacgaatt ctccgcccga acgaaagatc tcatggaaga
     3721 aatgatagcg accagctgtc tgcaatcagc cgtcagccct gagctgaaag ctgatagctt
     3781 ctaagcgagt acgccatgaa acaatcccgt cttcacaacc atcaccgcac gctgggcgcc
     3841 atatttgaag acgccagcgg ctggacaatg cccgcgcact acggcgatcc cgtcgcggaa
     3901 tatcgtgccg tgcgtcaggc cgtgggcgtg acggatcttt cccaccgcgg aaaagttcgc
     3961 gtcaccggtg atgatcgcgt gaaatggctc cagagcatca tcagcaacga tattctgccc
     4021 ttgcagccgg gccaaggccg ctactccagc ttcctcacgc acaaagggaa aatgctcacc
     4081 tacttccgtg tctacatgca ggcggattcg gtcatcctgg aagacgcagg ggaaatcggt
     4141 gatgcgactt acaccgcgct ccgcaagttt ctcctctacg ggaccaaagc gaagatggag
     4201 aattgcgcgg aaaactgggg gctgttgctc gtaagcggcc ccaaagccgc cgtggtgatg
     4261 aacgccgcct tcggcaccga cgtggaagac ctgaaacctc tcagcttcat ctcagcggcc
     4321 atcggcggtc agacggcact gatcattcgc accgaggaaa ccggggaatc ggattacgaa
     4381 gtgctgattc cggctgatgg actcgtcgcc gcatgggagc gcctgatgga cgcaggcgct
     4441 cagcacggca tcaagccgtt cggcatccag gcgcacgaat cgctcaggat ggaagccggc
     4501 attcccaaag ccggaccgga tttgaacgaa gaaatcgttc cgccggaagc gaatcttgag
     4561 gacaaagcct tcagcctgag caagggttgc tacccgggcc aggaagtcgt tgcgcggatg
     4621 gacacgtatg gcaatgtgcg caggaagctg gtcggtctgg tcctgaagga ttcgactgtg
     4681 ccaccccacg gagccaaact cttcagcggt gatcgtgaag tcggctgggt cagcggtgcg
     4741 acacattcgc cgcacctcaa taatgcggtg atcgcttttg gttttcccct cagagacttt
     4801 tcggctccgg acacggcact gaccgtggaa gtcgagggga agcgccttgc ggcgaccgtc
     4861 aagccgctcc ccttctattc tcgatcctag agcacctact tttcaggatt tctggccgca
     4921 gagactgcgc gggcaaatgc cagcagactg gtccgttctt catcttcgag cgccagcaga
     4981 agatgcgcct cctcgccgtg catcaaacgc agactcagga acggctcttc gcggcgtttt
     5041 tccttattgt cccacatcgc atcgatcacc tggaccttgt ccagctggcc gaccgacacg
     5101 atgtcgtagc ggaaatatga atccccgatg acgatgtcga acacgacgtt cccggccgtg
     5161 agcaacagga cgttgaaccg gccctggtct tcgtagcctt ccggcagaaa cttattgatg
     5221 tggcagagcg ccaccttacg atcgcccaac gccgcccgaa ttttgtcctt caaggctgca
     5281 tagtcgatct gcaaggcctt ttcatcagga ctggtcgcgg ccaccgccgc cgcttgcgtc
     5341 ttttcgaaga tttcctctgc cgacatcaac cctgccataa tctcgtctcc ttatgacttt
     5401 gttactgttg attctgtcgc gcacgaaccg ctcgcgccac accgacgaac ccgagaccga
     5461 cccatgcaat ttccaaaaga atgaaccctg cccgctgatc cacgatggcg acaatgccca
     5521 ggaggagcgc ccccacgata ttcaacgtga gataccccgc gtgcaactcc cgccagactc
     5581 cccccagatt gagtccgtat gccgtcaaca ccatcaacgc cccgaccacg gaaatgattt
     5641 gcagcagcat ggaacgacgt gtgactccgg gataacgggc acaaaacgaa ggggtacggt
     5701 aaccggtgca acctgaagaa tgcaagagga tgttcttgga atcgagccgt tatcgggcag
     5761 caagccgatg agaaaatcgc gtctttcctg tgtcgtcgat aatggtcacg tcgaacgaat
     5821 ttggcgtcac ccgaaccagt ccgaagttgt aatagcccgc ttcattgatc aaattcttcg
     5881 gatgcaaggc cggacttgga ggagtcaatc gaccgggact cgccgacagc gggccggcga
     5941 tatactcatg gaaatccggg atgccgtcac cattggaatc gtagctattc gcctgcaccc
     6001 aatgaacatc cccgccgaga aacacgacat tctttatctt gttcaacatg atggcgtcga
     6061 cgatcacctg ccgctcccgc tcgaaaccgg ttccatccga cccaccggcc cacccgtcat
     6121 agccgggaac tgacgcaccg cctcccttcg gaatggagag cgggacactg gtcacaatca
     6181 ccttccacgt tgcgttggaa ccgcgcaagc cgtccagcaa ccatttcaat tgctgggccc
     6241 ccaacatggt cttggctgga ccatcctgat cagcattctt gctgcgatac tgcctcgtat
     6301 ccaaaataaa gagttccaaa tcggctccgt aacgcagcgc gcgatacatt cggtgcggat
     6361 cctccgaggg agaagcgatc ggccagtatt cccgcagggc ctgccggccg gccggcatct
     6421 gttcatcata cggcccggca aagttgttgc ggacttcgtg atcgtcccag atgacataca
     6481 ccggcaccgt ttccagaaac cggcgcaggg actgcgcacc gcgctgatac cggtggcgcg
     6541 cgcgatattc cgagagcgtc gacgccttga agtctgcgcc tggctcgttt ggtggagaag
     6601 gacaggtatc gtcgctgtag atcgtatctc ccaggaacac aaaaaagtcg agaccctgct
     6661 tctgcatcat gtcgaaaatc ggatacccgc ctgccccctg gcgacatcgt tgctggccgc
     6721 ccaagtcgcc gctccaggca aaggtcacgg gaaccgaggt agctgcatcc ggcaaggccg
     6781 tgaattccca tttcgctgca agggcggcat cgatgtgatt cttagtatcg gaagcccgtc
     6841 ccacaaacaa gtagaagcga tagcgcgtgg cgggactcag cccttccagg ggaatggtga
     6901 ccgtgaagtc cgtgtccaca gtcgtcgcaa gtcgggccga ctttgcgacc ggagcaacgg
     6961 ccgtcgccat cttggaagcc ttctcccaga cagaaggagg ggcgtattcc acctgcacca
     7021 gagccggccc ttcggtgcgc acccacagca aggcgccatg agcagttaca tcccccaacg
     7081 caataccttg cggcaagacg tccggcatca gaacaaccgc ttcagcaaat ggactccctg
     7141 gcggaagcac atcccgcgac gccgaggcgc aaccgatcgg gggaaagaat atgagcagga
     7201 aaacggggag gagaaatggg atcaccatag acctagctgg ttccagaaaa aatcgagttt
     7261 cgttcaagag gccgtcacac gcttacgcac cgtgacaacg caacaacatc tcgcaggatg
     7321 ctcaaaaagg ccgtccagca aggccgcagc gagcgaagag gcgacgcgta gtctgtcccg
     7381 tacgtgagcc tctgagcgat gcgagaacgc cgctggcagt ctttttcagc agcctgctag
     7441 gggacttacc cgaaaggcca aatcccgtca aggtcactgc caggagtgcg gttgaaaatc
     7501 ctcaggacgg tcctgcatac tagcgcatac taaaggagcc ctgatgaata atctgaattg
     7561 tttgatccaa ggctgccaag ggggggtcta ctccccggcc ggcacccaga acctgtgcaa
     7621 ggaacatttt ctcagttttc tcacctggcg ccgccgcaga ggcccgcaga tgttcaccac
     7681 ctatgccggc atgaccatgg agcaacgcga tcccatcgtc gccgaatggg ccaagacgat
     7741 ccgtgtggaa gaagtgtcga cggctcctac aaaaacctga tcccatcatc tcaatcatca
     7801 aaagccttcc ctgctctttc tgtcatcaga ggcagatcga gtcttagaat catttcatat
     7861 cggtttggat gctctgtagc cctaatctct ctaggagatt tttcaaattg acttttaccg
     7921 gccgattagt ctcgagatta tcaggcccaa ccccgacgaa tgcggcagac acaagcttgt
     7981 agaagtcagc cccaaagagg aacagttttt ccattacatc ataagaaggc aggctatcaa
     8041 taatgacacc atactcacta tgagcagcct tcttatctcg aaatgtttta aaccgctcca
     8101 gctcatcttt gtattttgtt cgaaatactt cgaccgtgtt ttgcagcttt ccagccaaac
     8161 tgtctgcgct tgagacacaa ccataattaa gaagaaattc tttcaccttt gattcatcca
     8221 gacatgatgc tggttgagtc attaagtacc ttatgactcc ccttatggat tccagttcat
     8281 actgcttttc gtcctcgaat atcttacaga tacataacac aattgactca atagcccgca
     8341 tctggatgtg tccaaagaag actcctccgc cacgctcctt aattgatgcg gcgtttacgg
     8401 agatcaaaca atagacatct gagtcctgcc gtagcccgta aagaaccttg gccagaccaa
     8461 tatttttgcc gtcaacaggc gttccaatga gacccatacg cttaactaga gcaagctgca
     8521 ggccattttg tcttattgcg gctttgactt ccctcttcca ccaaatttct cgactcccaa
     8581 gggcctggca gagagcggag cgacttcacc gaaggagcgg ggccccttcg agactcagcc
     8641 aggaaggaaa gcggaaactg accgagtccg cgagggaatt tccgaaggct gccgcctggc
     8701 tgagtccgaa agggtcgctc cgagaggtgt agagcgagcc actctgccag gcccaccccc
     8761 taccccatca ccgcgcttcc gtgatggtgg atcatcaacc actcatcgcc gatgcgctca
     8821 aacaggttcg tggcgaggat gcgggcctgc tgcgcttcat cggattgctg ggttgtaatg
     8881 ttttcaatgc agatcaccca ggccatatcg ccggccacct gcaccatcac ttcggtgagc
     8941 tcgaacttca tcgagaacgt gttgttgaaa atgagcaccc aggagtctcg cacgttcggc
     9001 cagtctgaac gaagcgtcca accaggatgc acgcaggtca cgtactcgag atgggcccag
     9061 acctcgtcca tcttggcgat gtccaaggtt tcgaaggctt cgtaaaatcg ctgattggct
     9121 ttggtgactt cttcaattcg ctgttcaatc acgctgcccc cgtttctgag cgggctatca
     9181 tactggaatc agcgttcgag ggcgagatag aaacagaggc gctaggttcc ggcaagctga
     9241 cttgtttggc ggtgaaccac ccaaacgccc gacagaatca ggacgatccc gatgatttcc
     9301 acaagaccga ccgactcctt gaggatcatg gcagacagca ccacggcggc gaccggcgtc
     9361 agattcccca gtactgaggc ccgcgaagga ccgatgccct taaccccgaa caaccaggcc
     9421 tgctgagcca ccgcggtggc aaacacgacc agatagccga gcgccagcca atccttcgct
     9481 gccacggacg cgactccgga ctgtaccatc ttctgatcgg tccagagcag aggaatctgg
     9541 agcactgtcg ccaccagcag cgtcgtccaa ttcaccgtga gcgccgacaa gcgatccatc
     9601 actccgcggc ttccgatact atacagcgcc cagctcacga cacccagaaa cacgagcgtg
     9661 cccccaagca gcggctgttc gcccgcagcc tgaaatcccg ccacggacac gagcccgact
     9721 cccacaaaag acagcagacc gcctgcccac accgctcgca gcgggacatc tcgaatcatc
     9781 agcgcggaaa gcagcgccgt cacgaccgga gccgatccga tgatgacccc cgcgacggcg
     9841 ccgctgacat acttgagacc gatgaggatc agcagatgat tcccgactac gccaagaccg
     9901 aggagcgcga gcgtccgaag atccggtcgg gtgaggctca ccatccggcc ttcctgccac
     9961 caccagatgg acagcaagat ggcgaggccg ccgatatcac gcagaaccga ggcttcgacg
    10021 gccgagaatc cgctcaaagc cagcttctgc gccacaatcg atccgcccca gagcaccgcg
    10081 gcggcgacaa tggctccaga ggcggcgatg tccgtcagct ggttcatctc acgggcctat
    10141 agcaacaggc tgcgggatcg tcaagcagtt tctcgccacg atccacaaga gttctccata
    10201 ccactcgttc catgtcacgt tgtatcattg cccaccagga gctcaccttc atgaccatgg
    10261 cctcctcggt ccggcgcgct tgaacaatgg aacctcagca gatcataatt tcccatggcc
    10321 cagagcccct cacgatgaaa cccgctgaga aagaatggtt ggtcgacgga accatggttc
    10381 tgctcacgac ggccctgttc gttctcgacg tctatacccc gttgagtttc gccaaccatt
    10441 ttctctatgc cacggtgatc ttggtctcga ccgcctcgcg cgtttcgttc atgccgtggc
    10501 tggtcgcagg agtcacgtcc gccctgacgg tgatcggtgg attcatcagt cccccattcc
    10561 cggatctgcc gatgtggatt cccatcggga atcgtgcgtt cacgattatg atcgtctgga
    10621 tcctcgcatg gtttgccgtg aaacggcggc aggcggagtc ggccctgcaa cagagcaacg
    10681 aatgtcttga ggaaaaagtg attcagcgga cccgcgaact cgccggcgtc aaccaggcgc
    10741 tggtctccga agtcaccgag cgcatgcaga cggagcaagc gtatcgacaa tcggaagggc
    10801 gcctcgcggg cattctggat atcgcggagg acgccatcat cgtgatcgag caggatcgat
    10861 cgatcgcatt gttcaaccaa ggagcctcga aattgttcgg ctacgactct gccgacgtcc
    10921 tcgggcaatc gatcgaccgc ttactcccgg aacggttcag actcgaccat gcccgtcata
    10981 tccaggggtt cgctcaaagc caggaaccgg cacgccggat ggccgaacga caagaggtct
    11041 ttggcctcag aaaagacgga acggaatttc ccgcagaggc aagtatctcg aaactcacgg
    11101 tcggcggcaa aacgaccttc accgtcattt tgagagacat cacggagcgt ttgcaaaccg
    11161 agcatcaact gcactcactc gccacgcaat tgatgacggc tcaagaggaa gaacgccggc
    11221 gcatttcccg cgagctgcac gacgatatca accaacggct ggcactgctg gcgatcgaaa
    11281 tgggtaattt ggagaatgag ccggcgctgc ctgcagacca ggcaaaacgg tcgttccaat
    11341 cgttgacgct gcggcttgcc gccatttccg atgacgtcag acgcatggcg taccgtttcc
    11401 attcctccat cctggacgac ctcggactct ccgcggccct ccagcgattg accgatgagt
    11461 tttcgacgag gacgggcatc aagaccgttg ttgtgcagga agacttgacg gagcccttgc
    11521 cgagagaaat tgcctcatgc ctgtatcgga tcgtgcaaga aagtctcgtg aatgtgacca
    11581 agcacgccca ggcctcgcgc gtggaactgg aattgacctg cgacggccag gagattacgc
    11641 tagccataca tgacagcgga gcgggcttcg atctcaatca gattcgagcg cagcctcacg
    11701 gcctgggact cgttaacatg cgggagcgcg tgcgatccgt ccacggccgc ttcgagatcc
    11761 ggtctcaacc gggccatggc acacatattt ccgtactgat accacttccg ggaacacacg
    11821 atgaaaaaac cacgagtcct cttggcggat gatcacaccc tcgtcctcga ggggttcaag
    11881 aaactgctgg aagaacattg tgaattggtc gggacagccg aagacggccg gacgttggtg
    11941 gaccttgcag cccagaccca tccggacttg gtcctgctcg atatctcaat gccgttgttg
    12001 aacggcatcg atgcggcgaa gaaactgaaa aaacttctgc ctgatgtgaa gctcatattt
    12061 gtcacgatgc acgccgatac tgcttatatc aatgaagcct tcaaagccgg cgcttccgga
    12121 tacttactga aacgatcggc cgccaaagag ctggtgcagg ccgttcagtc cgtcatgaat
    12181 aacagttatt acgtgacacc cctcatcacg aaggatgtca tcagttcgtt tctgatgccg
    12241 gggcagactc gcgtacccgc cgtagacgac ttgacggcca ggcagcggga gattctccaa
    12301 ctcgtggcgg agggattctc tgccaaggaa atcgccggtc aactcaaggt gtctcaccga
    12361 accgtcgaat ttcacaaagc caagatcatg gaactgctcg acctgcatac gaccgccgac
    12421 ttggtgaagt atgcggtcgc tcatggtttg gtcagcgctt cctgacctcc ttgggcacag
    12481 tcccccagtt catcgtaatc atttcattgc aacccagtag tttccagcct catcgccagt
    12541 agccttccta tccccttctc gacggtcgaa agcttatgct ccagcgcgtg acacgagcgc
    12601 ccacccttgg catagcgagg tgcaccgatg agacgtcttc gcattgtact tgccgacgat
    12661 catccgcaaa ttctatcgtt gctgcaggtg ctgttggaac cagactataa agtggtgggc
    12721 acggctgagg acgggcaatc attgatcgcg gcagcccaag ctcttcgacc cgacgtcgtc
    12781 atcaccgaca tcgacatgcc gtttttaaac ggcatcgaag cgacgcgcga attgcacaaa
    12841 ctgttaccgg attgccgtgt gatcgtgcac agctcgcaca gcgaaccgga aatcatcgca
    12901 gccgccttag cagccggagc ctcgggatat ctgatcaagg gatcgtcaca atcgctcatc
    12961 tcctcgatcc ggtccgtgat ccggcatgtc tcggcacatg atgatgcgcc taacgtgaac
    13021 agagtaccgg aacacgccat cccgcgcgag agccgtgctc ggcaggaatc ctcttgggga
    13081 aatctgtagc aggggcgggc gcagattcat cacccctcgc ggctggtatt aagcccgtca
    13141 gctcgatgag cggatagtcg aaccgggctg aacaaaagtc gaggagtagg agggcgttcg
    13201 atgcaatgtc cccgctgtcg gaacctcgtt gtggaggaag tgtttgtcga ttacgagtcc
    13261 gatggtgcga tgtcgttcgt cggattccgc tgcgtgatct gtggaaatat tctcgatgag
    13321 acgattcagc gccatcgtgc cgagcacggt tcgtcgctct gcggcaaccg tcggaggcat
    13381 cccctgtcga tgagacccga acgggagacc cgcgaagctc gcagcgatga gtcgaaggaa
    13441 tatcacacat gagattagga gaagctgtgc gcacagcatc gacgagacag gaggcaccga
    13501 ccgccatgga gaaggcaccc gttcgccgac gtatcttggt catcgatcac gacgatgacg
    13561 ttcgggcatt tttatgcgat cggctcagcg ccctcggctt cgaggtggca gcggaaaaca
    13621 acggaatctc cggcctctcc cgtgtagccg acggccgtga cgctgccccc tttcatggtg
    13681 tactcgtgga attgcagatg ccggtactgg acggattatc cgtcctccgg gagatccacg
    13741 cgcgatttcc gacgataccc gtagtggtca tggccgattc catccatgtc ggcaaactcc
    13801 gccaggccat gacggctggt gctaaagagt atctggtcaa accattcgat ccggaactct
    13861 ttcgacgcaa gtgcgtgaac gtctttctcg acgggaagga actggcacgc tgaaacagga
    13921 gaagagaaac gtgaatggct gggtcagggg gtcggcaagt tcctgacacc gtgcgccaaa
    13981 ggcccgaaag gcccccctcc tatgacggtg tccggaggcc gcgcgtgttg gtcactctgg
    14041 cccggcctct aatcaatcgg cggaacgaac gccaacgggc gaggaaggag cgcagtaccg
    14101 atggaacctg ttgtcaccct ctgtgctctg acagcattca tatgggcggt cgcgatctgg
    14161 gcatcatggg agattgaacc tccggacagt gcgccgggga cagaagagga gaaaccggaa
    14221 cacatacctg aagaaacccg gaaagcggcg tgatgactga acttttcaca cgcctgtttt
    14281 tgagggctac ggcatcaatg tgaagcgcaa cgtgaagaag acgtcgtcgc gtgctgttcc
    14341 atcattcctc catcaccgat gatggagcac gtacggtcag atctctagca aatccctctc
    14401 acaataagag gcaggcgctg cgacgctcag gcgactggga aaaggagagg tttgctcgat
    14461 ggtgacacag caggcgatgt ggattaagat gtgggaacgt caaaccaggg cccggatcgg
    14521 gctacgcggc agactggctg tggcgagtct cctcagcatc ctgctatccg ggatactcag
    14581 ctgcacggca ggcccccatg caggaggatc gggcgctgta cgctacattc aaattcgcca
    14641 cgatgtcact ccgtccacgc tttatgcgca agtcggagac gagattcggt ggcacaattt
    14701 atcgcaagat cctgtccggg tgggcatact cgacaacaag tggcgcgatc atgtcgcatg
    14761 cgaaaagggc ttcacacgat tcggagcgat ggaagacttg gtcacgattc aaccgcagga
    14821 gtacgtgagc ctctgcttct cgaaagcagc caccgttcgc tacaacgtct ggttgcatgc
    14881 acagaacctc aaaggagcca tgagccccac agcggcgatt cggattgatt gaggccgctc
    14941 acatgccggc agccatctca caaaacgtcg gacaacgcgt gctgaggact ctcacgagca
    15001 tgcgtatgag catgttcatg ccgaacatgc tgatcgcctg gcgtcaggga acatcaccca
    15061 tgaggttcga cgtgcccgcc cagagaacgg aggccgcgcg aggtctcccg gcctcaggcc
    15121 ggtaggatag atgtcagaaa ggaccgataa gatggcgttg cgatttcgat tcatcttcac
    15181 atccctggcg atcgtgtggc aaatgggcgg cctgtgcggc acacttgcag cgtccgccga
    15241 atctggtccc caggcatacc agctaaatgc cattgtcgaa ctggccctgg ccagaaaccc
    15301 tctcatgtcc tccgcagaag ggcacattga ccagcagcgc ggccagcaga ctgcggccgg
    15361 cgcctatccg aatccaaccg tcaccggaaa tatcggttat ggtgagattc gagacgccgg
    15421 tcgcgcagac atccaaagct cgctcgatcg acggtcactc accgaataca acgtgaccgt
    15481 cggacagccg ttggaatggc cgtcgctgcg agcagcacgg cagcaggtgg cagaaggtgg
    15541 attagccacg gcgacggcca gcttgtcgga aacccgcctg aacctgacga cacaggtcaa
    15601 ggtggcgttc tacgacctgc tgttggccca gcaggatgcc gccctcgctc gacaaaatct
    15661 ggacatcgtc gaaagtgtgg cgcggatcgt caaagcacgg gtaaagtcgg gagaagggcc
    15721 tcaatttgaa ttgatcaagg ccgaagtcga agtcctgaag gcgaagcaac aactcacacg
    15781 tgcagacaac gtcgtccggg tgaaccgtgt cgtggtcgat actctgaccg gcggcgcact
    15841 cgggccctcc tatgtcgtgc agggcgattt cgccgggatg tcgaaggaat tgcacgtcga
    15901 agagttgatg gcccgtatgg cgatccggca tccgactatt caacgcctgc tgaaatcggt
    15961 ggaacagtcg gactggaaga tcgagtacga gcggcagtca cgggtgccga gtgtgacggt
    16021 gaacggcagt tattggcggg agatcggacg ggaagcggtt caaggaggtc tctccgttcc
    16081 catgccgctc tggtaccgcc ggcagggaga aatcgcctca tccttgggca cgaaacgccg
    16141 cgaagaagcg gaactgctgc gggcacgcaa tgaactggcc agagccgtcc accagcacta
    16201 tcaggacgcc agaaccacag ccgacctcat cgaagtgttt gacaaaggcc tcttgaaaca
    16261 agcacaggag gccctgcgac tcgcccagtt cagtttccag caaggcgcat cgagcctctt
    16321 ggaagtcttc gacgcgcagc gcgtgcagcg ccagatccaa atggattacg cacaggtgcg
    16381 atatgaattg tcggtgtcgt tggctcgcct cgagcgagcc gttggtggaa cactatgaac
    16441 ccagttagtc tgtcccgaaa cgtgtcatgc ggcagggaag aacgagccaa gaggacaccg
    16501 tccccccgca ctttcgccgt agtctacggg ctactcgccg ccctggcggc gatcgggtca
    16561 ggctgcgaca gcaatccgag tgatgcagtt gcgacgaagc cgcgggctgt cgcctccgcc
    16621 cccggagtcg tccttctgcc gcctgaggaa tcgtcgcgta ttggactcgt cgtccagccg
    16681 gccactcgaa gcgacttccg cacctaccgc gagtttcctg ccatcctcca accgaacgag
    16741 cgggccatgg cagacatcac gaccctagtc cgcggccgtg tggtggatgt atacgcggat
    16801 ctgggccaac aggtcgaagc caacgccctc ctggccatct tgtacagcag cgaactgggc
    16861 ctggctcaat ccgcctatct gaagtcgcgg gccaagcttc acgtcgcaga gcaggcctat
    16921 gcacgggcca agttcctact gaaagaaatg gtgatcggcg aggcggaggc tcaacgccgg
    16981 caggcggaac tgctcagcat gcaggcggaa gcccatgagg cgcgcgatca cctgaaactg
    17041 ctcggcatga cagatgacga atttcgtcgt ctcgacaaga gccgggacat tcgatcccac
    17101 gtgccgattg tggcgccctt cgccgggcgc atcatcggac gcaacctgac ccggggggaa
    17161 gtcgtcgaaa cgaccgagaa actcttcgtg gtggccgacc tgtccgaagt ctgggtacgg
    17221 gccaacatcc cggagaaaga catccccttc gtccactccg tccatgcatc cgggggcacg
    17281 caggcggatg tccgcgtcaa tgtctatccg agagaactct tcaagggcac gatcacctat
    17341 gtgggcgatg tcttggatcc ggccactcgc accatgcagc tccggctcga gctccccaac
    17401 cccgagaggc gactgaagcc ggaaatgttc gcgacgattc gactctattc cgaatcgcag
    17461 ccggaccggt tggccgtacc ggaatccgcg ctgcaacggg accaagaccg gacctttgtc
    17521 ttcgtgcaac ggaatgtgaa cgagtatgaa gtccgcgagg ttcagatcgg tgaatcaaac
    17581 ggtacccaca ccacgatcct tgacggcctc agcgaaggag atccggtcgt gacgcacggc
    17641 gccttcgtct tgaaatctga gctcttgaag aaacaggtct gatggtttct cgtctcctcg
    17701 acatttccct ccgccaacgc atgctcatcg tgatcttctc gatcatgatc ggggccgggg
    17761 gcatctatgc gttcagaacc attccgatcg atgcctttcc cgacgtcacc agcgtgctgg
    17821 tacaggtcgt caccaaagca cctggattat cccccgcgga agtggaacgg ctggtgacct
    17881 atccgattga actgcaattg acgggggtcc cttccctgac ggaaatgcgc tcgctgacca
    17941 aggtcggcct ctcgctcgtc acgatcgtgt tcgacgatgc gatggacatc aatctcgccc
    18001 gccagttggt gctcgagcga ctgattgagg tgaaggaact gcttcccccc ggcgctgagc
    18061 cgatgctggt tcccaacagc accggattgg gcgaggtctt ccaatactac ctggagggac
    18121 ctcaaggccc tgtcccggac gaagcggtcc agcaccagaa cttgatcgat catcgcacaa
    18181 ttcaggattg gatcattcgc ccacggctga agagtactcc ggaggtgatc gacgtcaatt
    18241 caatgggcgg gtacgtcaaa caatatcagg tgttggtcga acccgtgatg ctgcggaaat
    18301 ataacctcac cctccgcgag atcttcgacg ccgtggcgaa caacaatgcc aacgccggcg
    18361 gcaatatcct ggaaaaacat gccgagaaat acattgtgcg tggtgtcggg ctcatccgga
    18421 cactggagga cattgagcgc atcgtcgtca aggaagtcgg gggtactccg gtcttcgtca
    18481 gcgatgtcgc acaggtgctg atcgaccatg cagtgcggca cggcgccacc gtgttgaacg
    18541 gagagcggga agtggtgagc ggtatcgtcc tgatgctgcg gggcggcaac gcccgtgacg
    18601 ttgtcgaggg catcaaggcc cgcatcgacg acattcatcg ccagcacctg ctgccggacg
    18661 ggttgcgcat tgtgccgttc tatgaccgga ttgaattgat caccgccgcg ctgactaccg
    18721 tatacaagtc gctggcagag ggggtggtgc tggtcgtggt agtgctcttc ctcttcctgg
    18781 gcaacattcg cagcgcgctg atcgtcgtca gcacgctggt actggctcct ctggcgacat
    18841 tcatcgtgat gggccaggtc gggttgacgg cgaatctcat gtcgctaggt gggctcgcca
    18901 tcgccatcgg catgatcgtg gacggctcgg ttgtcgtggt cgaaaacgtc tatcggcatc
    18961 tctcaaccca ttcagccgtc gcggtgccgc ggcgagattt gattatgacg gctgtcaagg
    19021 aagtgggaca gccggtcgta ttcggcatcc tcatcatcat cctggtattc ctgcccgtgc
    19081 tctcccttca cggcatggaa ggcaagatgt tcaagccgct cgcctacacc atcatgatcg
    19141 ccctactggt ctcccttgtt ctgtcactca cgctgtcgcc ggtcctctgt tctctcatgc
    19201 tgaagcgggg aagcgaggaa gatccctgga tcgtccgttg ggccaagcgc ctctatgccc
    19261 cgtcacttca ctgggccctt ggcaacagga acgtcgtgct ggccgtggcg tttggatcat
    19321 tgctgtgcag cctggcgctg tttcctttcc tcgggagcga gttcattccg atcctgaacg
    19381 aagggtccgt cgccccgcaa accatccggc tgccgagcgt atccctccct gcctccattg
    19441 aaatcgaaaa gcgcatgcag caggccatca tggaattccc cgaagtggag atggtggtct
    19501 ccaagatcgg gcgcacggag ctgggcaacg atccgcaaga accgaacgag agcgatccgg
    19561 tcgtccggct tcgcccgctg gagcaatgga cgaccgcacg gacgatgccg gaactgatgc
    19621 agaagttcag ggagcggttg tcacggatac caggcgcgac gttcctgatc agtcagccca
    19681 ttcagcaacg ggtcgacgag ttgatctccg gggtgcggac tgaagccacc gtcaagctgt
    19741 tcggcgacga cttggagatc ttgcgaagca aagcggatga gatcgccggc gtcctgggga
    19801 ccgtccgcgg ggttcgcgac atcaaggtcg agcagctcta cgggcagccc tatctcacga
    19861 tcgacatcga tcggagcaag atcgcccggc acggcatcaa cgtcgccgac gtgcgggaga
    19921 tcatcaccac cgcgatcggc ggtcaagccg cgacccgtgt gtatgagggt cagcaacggt
    19981 tcgacctgtt tgtgcgtttc ccggagcaat accgggacag tcccgaaacc atcagtaaca
    20041 ttctggttgc cagtgggaat ggcgcactga tccccctggc cgatctcgga acagtgcgtc
    20101 tggaagaggg ccctggtcgg atcagccgtg aacagctcca acgctacgtg tcgatcggct
    20161 tcaacacgct cggacgcgat atcggaagtt tggtcgccga agcccagcaa aagattgcga
    20221 gccaggtatc tcttcccaca ggctacacgg tcgcctgggg aggttccttt gagaatatgg
    20281 aacgggcgat ggccaaactg cgcatcatcg tccctatcac gatcgggttg attttcctgt
    20341 tgctatactc taccttcgat tcgctacgtc aggccacgct catcattctg aacctcccct
    20401 tcgcgctgat cggcggagtg gttgccctct ggctgacagg ggaatatctc agcgtgccgg
    20461 cctcgatcgg tttcatcaat ttattcggcg tggctgtcct caacggcatc gtgctcgtct
    20521 cgtgcatgaa caaattacgc caggatggct gcagcctgga tgaagcggtc tttacgggaa
    20581 gcttgctccg cttgcgtccg gttctgatga cggcgctcgt cgccttactc gggttggtcc
    20641 cgctggcgtt tgcccgaggc atcggctccg aagtgcagcg ccccctcgcc attgtggtca
    20701 tcggcggact ggtcagttcc accctgctga cgcttgtggt gcttcccgtt ctctaccggt
    20761 gggtcgaggg gcgaagtggc gcgcgccccg agacagatcg aggaccgatg gaccttggcc
    20821 cgagccatga ggccgatcgg cagacggatc agatggatgg gcaaaggccc tctcatactc
    20881 atatacgcac acctcctccc cgccctctcc gccagtcgta acagtggaca tggatattac
    20941 gactgacctg ggaacacaaa ctaagctacg cgcgagccac gaatggcccc tcgccgtgag
    21001 cgtgatcact gccgccctgt tcctctcgtt cggatctcaa tggcttggtg atctttcgca
    21061 tccggcttgg tttgcctgca tgttgatgtg gctgttcacg gtgatcctgt tgtctgcctt
    21121 tgcggtgatc cggcatgcag aagtgatcgc ggaacagctc ggcgaaccga tcggcacgct
    21181 ggtactgacg ctgtcggtga ccggaattga agtcatgatg atcgcggcgg tgatgcacgg
    21241 cgggaagggc aatgcgacat tggcccggga cgccatgttt gccgtcgtga tgatcgtcct
    21301 gaatgggatg gtagggttgt ccttgctgct tggaggactc cggtatcacg agcagaccta
    21361 taaccttcaa ggagcgaacg cctttcttgc tgtgatcgtt cccttagctg tcttcggact
    21421 ggtgctcccg aactttaccg tgtcgtcccc cggcccgact ctgtctccgc ttcaaacggc
    21481 cttcctgatt atcatgtcga tcgggttgta cggggtgttt ctcgccatcc agatttggag
    21541 ccatcgcagc tatttcgtct tgcctgaaat agaacagggg ttaatggggg cgcacaggct
    21601 tcctggccag cacgaacggg gcggcctgtc tgttcgctat cccatcggga tgttgctggc
    21661 ctatctgctc ccgctggttg tcctctcgaa acaactggct attcccatca actatggcat
    21721 gcatgaacta caggcgccgc cagccctcgg cggattactc gtcgccattc tggttctgtc
    21781 gcccgagtcc cttggcgctg cccgcgcggc attggccaat cagctgcaac gttccgtcaa
    21841 catcctgctg ggatcggtgc tggccaccat cagcttaacc atccccgccg tgttgctgat
    21901 cggctttacg acgagccaaa taatcatttt gggactcgat gcggcagaca tgacgatttt
    21961 gctgttgacc ttggggttga gcaccttaac ctttgccagc gccaggacca atgtcttgtt
    22021 gggagcggtt cacctgctcc tgttcctggc ttacgtgatg ctgatcttcg agaaataaga
    22081 aataagatgc caggctcggc cctggtcacc atgttcgtca gagagaagaa gcaacaacct
    22141 acgcatggtc cggctttgtc cggtcatgac cgtacccttt gcaatcggat gggccgttgg
    22201 cttcacatat atgcgggatc tctcgtaatc tccgtgctcc cacctggtat ctttacgagt
    22261 ttcctccatt aagcaatgct ctagcatagg gttgcgggac ttcagtggct ccggaggagg
    22321 cgccgaatga ccatcgcaac catggccgat cgtgttcgtg gcgcgctaga aacgcgacaa
    22381 acagattatc cgatggatga aattgtggcg ctctgtccgg accttacctg gagtcaagtc
    22441 ttcaaggccg ttgaccactt gagtcggacg ggtcaggttc gcttgcggat caatcgcagg
    22501 cggacataca tggtcaaggc acttcgattg ccgatgccga tccacccttg tgccgattcg
    22561 attcccaagt aagccttgcc atccggtagc cagcactccc cgctctgaag aacagaaccg
    22621 gaacggacga tcgcacgact taccgagatg tcgagctggg cggctgagag gaattacccg
    22681 ttgcgcagtc catccttcgc aaggaacttt acaatggcgg gctggcatga acgctcaacc
    22741 tgaacctgca tcgaagcgaa gctagacgca taagttatag ctcgactgaa tccatctttt
    22801 gactgccgag atggtgaacg acacgatcca gtagacaccc atcttaaggg aacatgactt
    22861 cagtctttag atggaacgtc cttcaatgag agcaaacgtc ctagaggcga aggagacagc
    22921 cggcggatga gggaccgttt gcgcagataa aatcgattcc tgcactccct gcaacgccat
    22981 gggaacagac cgaacagtcg gcaactgaag tcccgccagc cacgccgacc ggaccgataa
    23041 caatccgcgg aagcacagta ggggcaattc acgacatcat ttcgtggcat ctgaccactt
    23101 cgtacggggt tacaccgatc agtagcgcca acacgtttcg catgtgtaat gcactaggat
    23161 ggcgaatcac cgtgccctcc ttttatcgga ccatgagatg atacggcacg gagtctctcg
    23221 gtgagtctta gcgacaatag gctatgcttc caatgtcgtt tgtcatatgc ctcgaaagag
    23281 ggagagtgaa ttctagtagt cattaggctg acgcgtacgc aggcaggtgg taagccgcgg
    23341 gccgccgggc cagcgcacat cagctatgct tcgtgtttcc tccacttccg cgtgcacgct
    23401 gcagctatta atttggttcg agcagaattt agtcacggtt gaaaacctaa tgggcagcgg
    23461 gtcttgacct atctgtacaa ttgatacaga ctagagcaat agcgttgagc agacttttca
    23521 gaggtttcgt cagccccatg aatggacgta ttgagacgaa gtatgtcgcg ttggtgctct
    23581 tgttcatctt ctgccaggtg atcggaacga tgtgtgcctt gcccgatctg tcggtggcag
    23641 aagaaatcgc caccttggtg gaagagggca tggtctgtcc gatggatggg acgatcatgt
    23701 gtccgccatc aatcacctcg tctcctgaac gtcagatcat gcatgttctc gtcgctgatt
    23761 tcgatcatgc gacaatcctg ttgggttcgg ctgcaggtct tccagttcgg tcggctcctt
    23821 cgctgtggtc ctggagcagc tcgtcttcca ttgtgcccat ctccataagt tctccctcgg
    23881 tcctccgaat ctgattcctc acttccagtg cgttcactcc ttgctcggct cctgtgcctg
    23941 agcgggtgct ggtccatttt ccgaacgtcg cctggaggtg tcaccgtgaa accatctatt
    24001 gttcccgttc aaggatatat cattcctctc gtcgtcttct cggccgtggt gctttcaccc
    24061 tggtccgttc gtggagcgga agatcgtggg atagtcgttc cggacgggac cgctcatacg
    24121 tcgcatagcg agcttcgcat cgaccttccg ggtctcattg gagaactgga acgcgccaac
    24181 ccagaaataa aggctgcccg tcagcgctgg gaagcctcga aggcggtcgt gccgcaagtc
    24241 caaacactgc cggaccctcg cttgcagctt ggctatcaac gcatgcccat ggttcctccg
    24301 gttgtggaag gcgcgatgta tgggttcgga caggaaatcc cctttcccgg caagctcggt
    24361 ctcaaaggag aagtggctca gcgtgaagca gagcgactgg agcaggaatt caatgccacg
    24421 cggctgagac tcattgcgac tttgaaggag gcgtatttca acctgcactt cgtgcacaag
    24481 agcatcgaga tcgtggagag aaacaaagtg ctcttaatgc agttcgagaa aaccgccaaa
    24541 gcgcggtaca gtgtcgggca ggccgcacag caggatgtgt ttcgggctca agtggaaatt
    24601 tcgcgtgttc tcgaccggct ggcggttctc gatcaacaga aagaaagtct gcatgccgcg
    24661 atcaatcgcc tcctcaatcg tccgcccgcc ggaccactgg gggcaccagc agaaattcaa
    24721 accacgattt taaccatccc gcttcaggac ctgagccggc gcgccgatga attctcccct
    24781 gcactgctgg ccacggcaaa aagtatcgac cggtccgaac gatccgtctc actcgccaaa
    24841 cgtcaatatt atcccgattt cgatatcacg gccctcggcc tccgaaacga caaaatcaac
    24901 gataacggtt accaggtgat ggtggggatt aagattccgc tcttctatga aaccaaacag
    24961 aaacaaggtg tccgagaggc gcttgccggt cttgagggag cgaaagagga cttcgccaca
    25021 actcggcagg atctgttgtt ccaggtcaag gacggctttg tccaagcgca gcgggccgag
    25081 cggctgatca ctatccttcg cgatgcgatc atcccccagg ccacactggc cttgcaggcg
    25141 gcgcaagcga gctatacggt ggggaaagtg gatttcctga cgctgctcaa cagcctcttg
    25201 attctgcagg acagccagct cgaactgcat ggcgagatgg tgaaccacga gaaagcgctc
    25261 gcccggctcg aagctgtgac cggtggaccg ttaagtcgcc cgctgtcgga aaggaaaccg
    25321 ggatcatgac acgtcagcgc cttatcggta tggcagccct gctcatcctc gccgcagtcg
    25381 cagtcgtctg gctgacgtcg agaccgctga tgcttccacc ggcggattcg agacaggacg
    25441 acatggccgg gatggacatg ccagggatgg gaaaggcgca gcagcagaag tcggccccgg
    25501 cggagaaagc ggttggacca gagattccac tcgagtccga aggggtacca ggcatcgtaa
    25561 cgattccacc ggaacggctc caaacgatcg gcgtgaagta cgagcaggtg gcgcgtcgtc
    25621 cgttggagaa ggtcattcgg actgtggggc gtgttgtcat ggatgaacgc aaactcgcca
    25681 aagtcacgat caagtttcac gggtggattg aacagctgtt cgtgagtgcc acgggggatc
    25741 atgtgaagaa agggcaacga ctctttacga tctacagccc cgatctcgtg gcaacacagg
    25801 aagaatacct tctggcattg cagagtcgga agcagctggg ggaaagcgag attccggaag
    25861 tcgcggcggg ctccaaggat ttgctggaag cgactcgtca tcgcctgcac ctgtgggaca
    25921 tcggcgaaga gcacatccgg gaattagaac ggacgaaaca ggtgaccaag acgctgccga
    25981 tccattctcc gatcagcggg accgtaattc gcaaagaagt tctgcaagga actcatgtgg
    26041 agccggggga agaactctac accattgcgg atctctccca tatttggatc ctggcggaca
    26101 tctacgagta tgaactgtcc ttcgtaaaga ttggccaaca agccgccgtc acgctctcct
    26161 atgaccccag cacggtgctg accggtcaag tgggcttcgt gtatcccaca cttgatccca
    26221 agactcggac ggccaaggtt cgtttcgaac tcgataacgc ggacgagaaa ctgaagccgg
    26281 acatgtatgc caatgtggag ctacgcgtga acttaggtac tcgtctggcg atcccgcaag
    26341 aagccgtcat cgaatccggc gagaagcagg tggtgtttct tcacctgggt ggcgggaagc
    26401 tggaaccgcg cttgatcaag accggcgtga agaccgggga gtggtctgaa gtgctgacag
    26461 ggttgaaaga aggggaccac attgtgacct cggccaattt cttgatcgac agtgaaagcc
    26521 ggctcaagtc cgtcgtcgaa gggatgggcg gcatgtccgg catgaagtcg aaggattgag
    26581 cgggtcatgg tcgaacggat aatcgagttc agtgcgagaa atcgcttcat cgtctttctg
    26641 tttgtgtttg cattgtcatc ggccggtctc tgggccacgc ggcatacgcc ggttgatgcg
    26701 ctcccggacc tctctgacac gcaggtgatt gtctacacca cttgggcggg gcggtctccc
    26761 accctggtgg aagatcaggt cacctacccc attgtcacgg ccctgctgtc tgcccccaac
    26821 gttacggtgg tgcggggatt ctcagacttt ggctactcct atgtctatgt cctttttaaa
    26881 gatgggaccg acatctactg ggcccgctcc cgcgctctcg aatatctcaa tcagctcgct
    26941 ggtcgcttgc ccgaaggcgt gaccccccag ctcgggcccg atgccaccgg cgtcgggtgg
    27001 gtcttcgagt atgcgctcgt ggatgagacg ggacagcacg acctcgccgc cctgcggagt
    27061 tttcaagatt ggtatttacg ctactggctg cgcggtgtgg agggggtggc cgaggtggcc
    27121 agcgtgggag gctttgtccg tcaataccaa gtgaatttag atcccaccaa agtcctcgcc
    27181 tacaagctct cgctgtctgc gatcatcgag acgatccgac agagcaacaa cgatgtcggc
    27241 ggccgcgtcg tggagtttac cggtatcgag tacgtgatca gaggacgtgg gtatattcag
    27301 aacacgactg acatcgagaa gattgcggtg gcggtcaatg aaaacggtac gccgattttg
    27361 ctgcgtgacg tggcgaccgt gcgcctgggg ccggacatgc gaaggggctt ggtggatctc
    27421 aatgggcaag gggaagtcgt gggggggatc gtggtcatgc ggttcggcca gaatgccctg
    27481 gaggtgattg accggatcaa ggccaagctc aaggaactgg aaccctccat gcccagaggc
    27541 gtcaaggtgg tcaccaccta cgaccgaagt gacctcatcc gtgagtcgat tgggaccgtg
    27601 aaggaaaatc tgattgagga attgctcatc gtgagcgcta tgctcgtcgg attcttgctg
    27661 cacctgcgtt cggcgctgat gccgatcctg actctcccga ttgctgtact attctccttt
    27721 ctcccgatct ggtacttcgg ggtggggatg aacatcatgt cgatcggtgg gatcatcgtg
    27781 gcgattggcg acatggtgga tgcatccatc gtcatggtcg acaatgcgca ccgacggctc
    27841 gaagagtggg agcgcggggg ccgtgtcggg gaccggacgc aggtcctgat cgactcggcg
    27901 aaagaggtcg gcccgtcgat cttcgcctcg ctgttagtga tcgccatcgc ctttatgccg
    27961 gtcttcacac tggaggccca ggaaggacgg ctgtttcgcc cactcgcagt caccaagaat
    28021 ctcgcaatcg cgatgagcgc ggtgctggcg atcacgctca tcccggcatt gttggatttt
    28081 ctggtgcgtg gccgcatcat ctccgaacag cgccatccga tcagccggac gctgcagcgg
    28141 ctctatgcgc cggtcctccg gttcgccctg cagtttcggt gggcggtggt cgtcggcgcg
    28201 gtcctcctca cgttgctcac ggtcccgccg tttatgcgcc tcggctccga gttcatgcca
    28261 cctctctacg aaggcaccat cctctacatg ccgacgacgc tgccgggtct ccccgtcacc
    28321 gaggcgggcc gattgctgca gatcatggac cagaagctcc gcgcgtttcc ggaggtggcc
    28381 tgggtattcg ggaaagccgg gcgcgccgag acctccaccg actccgcgcc gttcggcatg
    28441 atggaagtcg tggtcgagct gaatccgaag gaccagtggc ggccgggcct cagttacgag
    28501 gggttagtcg atgagatgga ccgggcgctg caactccccg gcgtgaccaa cgcctggact
    28561 atgcccatca aggccaggat cgacatgcta accaccggag tgcgaacgcc tgtcggcatc
    28621 aagatcttcg gccccgatct caccgagatt gggaaaattg gcgagcacct agagatggtg
    28681 ctgaaagaag tgccggggac ccggagcgtc tatgcggaac gggtcttcgg cggctattac
    28741 ctggattttg tcattaatcg ggacgagatc gcccgctatg ggctcaaggt catggacgtg
    28801 gggcggatca tcgagtccgc tattggtggc gaaaatatcg ccacgacgat cgaaggtcgt
    28861 gagcggtacc cgatcaatgt gcggtatctg cgtgaactcc gcgacgagcc cgagaaactc
    28921 cggcgcgtcc tggtcgacac gccgactggc gcacaggtcc ccctcgccca actggcgacg
    28981 ttgcggttcg tcaacgggcc gcccatgatc cgggacgaaa acggaatgct ggccgggtat
    29041 gtgtttgtgg acatggtcgg ccgcgatatg gggagctatg tggacgacct caagcgcgtc
    29101 gtccaagaga aagtgtcact ccccacgggg tacacgatca cctggtccgg gcaatacgaa
    29161 aacatgcagc gggtgcggga gcgactcacg atcttcattc cgctgacgat caccatcatt
    29221 tttgtcctct actacttcac cttccgctca gtcgcggagt ccctgatggt cctgctcggc
    29281 gtgcccctgt ccatggtggg cgggatctgg tcactggccc tgctgggcta caacatgagt
    29341 atcgccgtct gggtggggtt aattgcgttg gctggtgtcg gggctgaaac cagcgcagtc
    29401 atgctggcct acctggatga ggcgtgcgcc cgtcggcaga aggccggcag gctccgcaca
    29461 ctctccgatc tgatcgagac ggttcaggaa ggggcgctgg tgcggatccg agccgtcacc
    29521 atgacctcgc tggccaacat tctcgggcta ctcccggtca tgtgggccac cggaaccggg
    29581 gccgatgtga tgaagcgtct ggccgcgccc atggtcggcg gggtgttcac ggccatgctg
    29641 ctgaccctgg tcgtgattcc cgcactttac gtgttctggc gctggcactc cgatgtgaag
    29701 caatcaggga tatgaggcag gagacgagag gacgaaatga gtaatgacac caccaaagat
    29761 gcggaactct ggtttctcgg tgcaattgaa gcttctcagc atgatgatgg tctcatgaga
    29821 gggaagggaa atcgatcgag cacatcggaa caaggaggga agcgcgatgg ccaaagatcc
    29881 tgtatgcgcg atggaggtag atgaccaatc tgccaagtgg accagcacgt atgaggggaa
    29941 gcgctatagc ttctgctcgg ctgggtgcaa ggacagtttc gacaaggatc cagacaggta
    30001 tcttctcgat cgcgatttgc ttggaagaaa gatcaagcct caataaggca gggggcgacc
    30061 tgagaaattg gcataggtcc acggttttgg gatagcgaaa cgtgggtctc tttcactaca
    30121 tgagtgcgtc cagtgctggc aagagatcag ttctaccgct tccgagacct agttaggagc
    30181 tatcggagag gatctctcca agtcgatagt ctatgtaagc cgcaggtccc aaatccagtt
    30241 cccgtcagct aggcgttgtg tttcctctag cactagcctt tctttctcac cacgagaatc
    30301 ccatctcgaa tagtgacgag aacagcggag actttcccgt cactggagac tgccttgttg
    30361 agttcttgaa tcgccgccgt ccgctgatct gggggtggat gttttaatac ttcaccgccc
    30421 cacagcacat tatcgatgag gatgatgccg ttcggcgcta acaattcgag agcccggcga
    30481 aaataattta aataattttg cttgtccgca tcgatgaaga ttagatcgaa tggcccgtcg
    30541 agattctgca tcgtttcaag ggccggcccc attctcacct ctactttctt gcctactggc
    30601 gaacgcgcaa aatacttccc cgcgagggta gctgactctt catctacttc gcaggtgatg
    30661 attgtgccat cttcgggaac ggcttcggca aaacaaaggg cgctgtagcc agtgaacatc
    30721 ccgacctcca gaacccgctt cgctccgaca acctgcgcca tcatcttcag aaacgctccc
    30781 tccagaggtc caacgagcat ctgtggaaac tccatacggc gataggtctc ttcgcgtagc
    30841 gcccgacaga tggccgattc cgccatcgaa tgggcctccg cataagcttc gatctcaggt
    30901 gtgaccaggc ttttcattgt tgcgcctctc tgacttgttc tggaccggtt ggaagttagc
    30961 agtcccccta ctgaacaatc gcgagcagcc ttcctaggct ttacagttca taagccattg
    31021 aaagaatgcc ttttgcatct taactgagca tccgtaactt aggagagagt acctcatcga
    31081 tgaaaatcaa gtcgactggg ccagtcaggt ccttcatcgt gtccagcgcg gggcccatgc
    31141 gaatcgacat cttcgtcccg cgcgtcgacc gtgcgaaata acgacgggcc aggaccgccg
    31201 atgcttcgtc aatctcgcag gttgtcaccc tcccgtcttc cggaatggcc tccgcgaagc
    31261 agagtgcgct gtaccccgtg aacatgccca cttcaagcac gtgctttgcc tgtacaagtt
    31321 gggcggtgat cttcaaaaac gcaccttcga ggggtccgac caacatctgg gggtattcca
    31381 tcacccgaca ggtctcttcc cgtagggcac ggcagacctc ggattccggc acggaatggg
    31441 cctcggcata agcctcgatc tcagcggaaa ctaacgcctt catggccacc atcctcctcc
    31501 gtttgaaaat cgggcatgag gtgcgtagac ttataaaatc tagtgcggca cgatcatagc
    31561 atgcataaca tccttgtgag tcttggagcc ctccgaacat gaagaccctg aaaaccctgg
    31621 aaccactcgc cacgaggctg ctggagattc agcgcatcaa cagcgccgct tcggtcctat
    31681 cctgggatca agaaacctac atgccggcag gcgggggttc agcccgcgct gagcaaattg
    31741 ccaccctcca gggcattgca cataaaaaac tggtttcccc cgacaccgaa cggctgctgg
    31801 ggaactggct cgatctcggc accggccaac cgctcgagca ggacggggaa ttgtgggacg
    31861 aatcttcccg cgccctgctc cgagaagtct ggcgggatta cagccgcgcc aagaaactcc
    31921 cgtcagactt tgtcatgacc atgagccggg aatgttcctt ggctcaacag gtctggattg
    31981 aggcgaaaga cgaacacagc ttcaagagat ttcttcccaa cctctgtacg gtcctttcgc
    32041 tcaagcgccg ggaagccgac tatctgggat accagggctc tccctacaat gctctgctcg
    32101 acctgtacga gcccggctcg accatcgcgg cactgcgtcc cctcttcgcg cagctcaagg
    32161 cgcgtctggt cccgctcctg aaaaaaatcc agaacagcag cgtgcagatc gacgacggat
    32221 ttctctacca cgcctacgac caggccagac aactggagtt cggccggctg gtgctgatcg
    32281 cgatggggta tgatttcgag cggggccgcc tggatctctc ggcccatccc ttcaccacgt
    32341 cgtttcatcc aacggacgtt cgcgtgacga cgcgcgtcca ggagcacgac cttccctcct
    32401 gcctctttag ttgcatccat gaaggcggac acggacttta cgatcagggt ctcgatccgc
    32461 tgcactacgg cacgcccctc ggcgaatcgg tgtcgctcgg cattcacgaa agccagtcga
    32521 gaatgtggga gaactgcgtg ggccggtcgc gatccttctg gcgatttttc tttccgatct
    32581 tgcaggcgaa tttccatcac cagctgcgcg atgtggactt ggagcagttt tatgccgtcg
    32641 tcaaccgggt aaaaccgtcc ttgatccggg tcgaagccga tgaactgacg tacaacctcc
    32701 acattatgct tcgatttgag atcgagcagg atctaattga gggaaatacg aagccggaag
    32761 aacttcctga gctctggaac cagaagatga aagagtattt gggcgtcgtt ccatcacacg
    32821 atggagaagg cgtgctccag gacgttcact ggtccatggg ggcattcggc tattttccca
    32881 cctacacgct ggggaacctg tacgccgtgc agttctacga tcaggcgaaa cttgaaatcc
    32941 ctcacatcga agacgagatt gccgcaggac aactcatggt tctacgccga tggctggggc
    33001 agaaaatcca tcgatggggc cgcatgttca cacccgatca tctcgcccaa cgcgtgaccg
    33061 gcgtcagcct cagccctgaa ccatttctgc gatatgcaga acggaagtac ggcgaattgt
    33121 atcggctcta gagggcagca agacgctact ccgctttcag ctctcctctc tcagccccgc
    33181 tctaatatcc catcgcctgg ttcaatttca tcaagagcgc cggagggaag gtgaattccg
    33241 tttgcacttc gatgcggtgg ggcacgagca gcgtcgtgtg aaacacgata tcgcggatgg
    33301 ggatcttaaa ttccggatct tgaggcgtgc tgagttcgac agcttccacc gaattggtaa
    33361 tcgccccgct atcgtgcggt ccataggtgg ccactaccgc tttcaatatt tcgatcactt
    33421 tctcattcgc ctcgacgccg gccactccct tttcgatcag cggcaggacc tcctccctgg
    33481 cctgatccga tagcgtgaaa ttctcaaccc gttccttgcg ccggagcgac gtgaggtcgt
    33541 tgtcgagatc cttgctgaac gcgccgtagg caaagcggaa gaattcgaag tgaaatccct
    33601 tgaacccctt cccgaacatc tgcagctcgc agagaaaaca caactgctgc aacttgacat
    33661 cgctcaacaa gccgtgcggt tccgcttgat ggaggagata cagcaaaagc gctcggtcta
    33721 cggtgatctg atggggctta cgcatgggtg cgaatccttt tccctcgccg acattcggcc
    33781 ccgcactata gacagtggaa ggaaatcccg tcaatcgctg cccccttgac cggctgatta
    33841 gtttcgtctt taatgacctc ccgtttggag gcaaacgaaa tggcttctgg caagcatgtc
    33901 aaagagatgg agtcgctgaa agaacatctg gcgaagcacc atctgaaatt cacccgccag
    33961 cgggaactga ttctcagtgc ctttcttcgg caggagcaca tcactgctga agcgatgtac
    34021 caccaactcg ccaagaccga tcctcatttg ggattggcca cgatctaccg aacgttaaac
    34081 ctcttctgcg atgccggcat cgcccaggcc cggcactttg gatcgcagac gcagtacgat
    34141 aacgtctccc acaagggcca ccacgaccac ctcatctgca cgggatgcgg gaaaatcgtg
    34201 gagttcgaaa actgtgaaat cgagaaactc caagaggaag tcgcaacaag aaatggcttc
    34261 acgattcaaa cccaccgtct ggaactctac ggactctgct cccgctgccg gcattgaccg
    34321 gaatcgcttt ttgtagtata attttgagat ggcttatcaa tttcaattag tgaagggccc
    34381 caccatgcta tttcgtagta cttggatcac tccgcgacga accaccgttg ggctcctcac
    34441 acttttgttc accattgggc tgatcctcgc cggcctcgta caggcttcgc cggctgccga
    34501 caagattaca gtctattctg gccgcgcaga gcgtttaatc aaaccggtgt tcgacgactt
    34561 tacggttaag accggcattc aagtcgagct gctgtcttcg ggtacgacgg agttggtcaa
    34621 tcggctcaaa gcggaaggcg accgcaccgc agcggacctc ttgatcacga acgatgccgg
    34681 cagcctcgaa ctggccagaa cagccgggat actccgcccg ctcagcatgc gtgaaatcga
    34741 ccgcgcgatt cccccgcagt tccgcgcgcc ggacaatgct tgggtcggat tgtccgggcg
    34801 attctggatt atcgtgtaca acacgtcgat ggtgaaaccg gaccaactca aatcactcct
    34861 cgacctcgcc gatcccaaat ggaaggacaa aatcgccatt cccaattccg gcagcgaata
    34921 cctgcaagcc ggtgtgtccg tcattcgggc gacacacggc gacgccaaaa cgaagcagtt
    34981 cctccaaggg ctcaaggaca atgccggcgg acaggtgtac caaaagagtt cgcaaatcgt
    35041 ggatgccgtg gccaaagggc aggtggcact ggggatcgtc aatcattact acgtctatcg
    35101 tcacctggcg actcagccgg ctgcccccat cgcggtcttc atgccggacc aacaggacgg
    35161 cggtatggga gccatcatga atgtcgccgg catcggcatc gtcaagtcga cgaagcatct
    35221 cgaaagcgcc aagctcctcg tcgaattcct cgtcgcccaa gcggggcaaa aactttttgc
    35281 cgaactcgac aaggagtacc cgctgcaccc cgaggtcaaa gccgatccag ccttggtgga
    35341 gcggaagagc tttcgcgcgg cgcaagtgcc cctgacgaaa cttgctgaac tgcgcgaacc
    35401 cactcttctg ctgatcgaac aagtgggcct ccgctaattc gaagggaccc actgtgatca
    35461 cgattcgcca gcaactgtcc tccccgctcc aactgatcgc agtggccagc gccggcatca
    35521 ttttgctccc gctcggctat gtgacaaccc aagcgctttc tgcggatccg gcggtctgga
    35581 gccggctctg ggcaacccgc attcccgaac tgctctcgaa taccatctgg ctggccgcga
    35641 cagtcgcctg cctcacactg atcctcggtg tgtccactgc ctggctcgtg acgcgcctgg
    35701 attttcccgg ccgccgcctc tgggaaggag ccttggttct cccccttgcc atgcccacct
    35761 atgtgctggc ctatgtctac tcatacctgc tgggattcgg cggaccggtc gagcatctgt
    35821 ggcaaatcgt cgcagggcca caagcgcgaa tcttttctcc ccacagctat ctgggcgcga
    35881 ccctggtgat gacactcgac acgtttccct ttgtctatct tctaagccga agtgcgctct
    35941 taagcatgaa cgtttcattc gaggaagtat cgcgcgccag cggtgtctct cgtgtgcaga
    36001 ccttgtggcg ggtgaccctc ccgctcatac gtccctcaat tgcggccggc cttgcgctcg
    36061 tgattctgta tgtcgtgtcg gatttcggag cggtatcgtt gctgcgctac caaacgctga
    36121 cctatgctgt cttccagcag atgaccggac gatccgacaa taccgcggcc agcattctga
    36181 gcctcctgct ggtcggactg gcgctgatat tcttggttac ggaacgatgg ttccgccatc
    36241 gcagccggtt ctatcaaacg accggacggt accggactcc acagcgtcat cgttatggat
    36301 ggcttggaag cttgctcgtc accggctacc tgggcttgat cgtcggcgcg gcgttcgcgc
    36361 tgcc
//
LOCUS       DATIFR010000200        16404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_135695, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000200 DATIFR010000000
VERSION     DATIFR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135695"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VLD60_11540"
     CDS             <1..186
                     /locus_tag="VLD60_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HSB45639.1"
                     /translation="YTSCNIEYAIPTDQVMADAKTVEDDRYKFAEYISEDAKAYLAER
                     YGYKEFVQKPALAVTQA"
     gene            287..520
                     /locus_tag="VLD60_11545"
     CDS             287..520
                     /locus_tag="VLD60_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45640.1"
                     /translation="MTDVKPTLTRSPHYTLSYGMRLDKGTGSELSHPHVPIILPNGSP
                     GSMTLHVMNGTPEEIKARLLESVDAFFEIYADL"
     gene            595..960
                     /locus_tag="VLD60_11550"
     CDS             595..960
                     /locus_tag="VLD60_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45641.1"
                     /translation="MQTLEIDRPDMPDLQFVLLVTALCTSRLTLLNIPHTLRAVIFDR
                     CWVLIHDSPPPRRPEERVLDLRPWTEVTVEAMVETICAALTEAGIQTLAWDHPASEPT
                     ITSTPEAQPLIDRLDRLYP"
     gene            complement(979..1779)
                     /locus_tag="VLD60_11555"
     CDS             complement(979..1779)
                     /locus_tag="VLD60_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSB45642.1"
                     /translation="MPGMDLIIEYAVRYGFQTLGAIIVMAVGVLVARWAGKLTLQWLQ
                     RQDMEPPVRMLLTKVVQIVIVVFALLAALGQLGVQIAPLLAGIGVAGLGVGLALQGVL
                     SNVVAGLSIIFTKPYKVGEHISILGVHGDVLTISIFSTILLHPDQSRIVIPNRKIVGE
                     ILHNFGTIRQLDLQVGVAYATNLNEALAVVREILAHHPSVLKDPPPFTGVSQLGDSTV
                     RIAIKPWTSVADYGTAEVELNQAIIERFRAVHIEIPFPQREVRLLSTP"
     gene            complement(1830..2684)
                     /locus_tag="VLD60_11560"
     CDS             complement(1830..2684)
                     /locus_tag="VLD60_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45643.1"
                     /translation="MMCHLNTSVGHRGACTAVAIIFVIIGLACSPADAVPIAVRFPEG
                     MTHGFLLVRSSAGEIIGQGEITQVVKEGDLVDSHLVFTFKDGSLHDEKVTFSQRHVFS
                     MLRYHLIQRGPSFPEQIDVSIDRGTALYKVRSKTAEGGKEEVLAGTFTLPKDVYNGIF
                     VTMLLNLPKGTSETVNFLAFTPAPEIIKLELHLMGEHPVRIGEQSRKAVQYVFKPDVG
                     MIREMVGKAIGKLPDNFQYECWILDDEVPSFVQFEGPLQLMGPILRIELMSPHLLTKS
                     EDKKSSSK"
     gene            complement(2883..3299)
                     /locus_tag="VLD60_11565"
     CDS             complement(2883..3299)
                     /locus_tag="VLD60_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45644.1"
                     /translation="MVMNIAHRMIGIALGIVLFAGSGLAAEPAEVEKFVRARIEIGEM
                     MTNYFKGGERFKEGERPSPEKMKEMSADINAKLSTVLAKYDLTVEAYRQRSPEVFADD
                     AAVKRFLGEHPDLKQRYDALQLDRMSRGGGSTGRGY"
     gene            3488..4057
                     /locus_tag="VLD60_11570"
     CDS             3488..4057
                     /locus_tag="VLD60_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSB45645.1"
                     /translation="MAVASVMLPLGTPAPPFSLCDVVSGQIYSLDSFTGKTALLVMFL
                     CRHCPYVQHVEQELARIGRDYRDASLGIAAISSNDPAEYPDDAPEQLKAQALHLGWTF
                     PYCFDETQNVAKAYRAACTPDFYLFDRERRLVYRGQLDDSRPGNNKPVTGRDLRAAID
                     ATLAGRPVDSRQQASIGCSIKWKRGNEPA"
     gene            4116..4445
                     /locus_tag="VLD60_11575"
     CDS             4116..4445
                     /locus_tag="VLD60_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSB45646.1"
                     /translation="MVAAACDGPMQTVSITAEDFRFVPDLAQVNSSSPLTLTIYNAGR
                     EIHEFDSPILMYAASTHSTAKQASPGLLLEPGKSVQLVMAPPPGTYLYICRRKGHANM
                     TGTLIVE"
     gene            complement(4535..5056)
                     /locus_tag="VLD60_11580"
     CDS             complement(4535..5056)
                     /locus_tag="VLD60_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45647.1"
                     /translation="MRSQQIGLWAAIGVLLVGIFLIDMQMALGFTPWQLYVIPLGLTY
                     WATLLYAPFFVAAISTILMLVGFDLSPPLVPESVAMTNRLIGAITFWGMASLIVAYKL
                     LAIRLSQLTEQLRLELNERTQDLGRVVIALRAEEGREQGLAPEGEDSLEKTRTDLEHL
                     GRQLEQLQRDLLR"
     gene            complement(5057..5245)
                     /locus_tag="VLD60_11585"
     CDS             complement(5057..5245)
                     /locus_tag="VLD60_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45648.1"
                     /translation="MSRVPTTSRPACVLGTPACPRCLETALESNGGFLTCATCGLAIT
                     RQALLNDTIGLQPALDDH"
     gene            complement(5464..5691)
                     /locus_tag="VLD60_11590"
     CDS             complement(5464..5691)
                     /locus_tag="VLD60_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45649.1"
                     /translation="MAFGSDLSFTESLQSDFRRTFYESHRSIAIVMVVIVFLSPFAGL
                     YVTGLFGAVLGMLLSVAAYYLTPYVVLSLVD"
     gene            6037..6348
                     /locus_tag="VLD60_11595"
     CDS             6037..6348
                     /locus_tag="VLD60_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45650.1"
                     /translation="MKMLQIVCGEKLGEEILAVFKGLGISGYTMISGVGGSGQTGTVS
                     GTGASTDRNTFFMVALEDTHIHMTQVLNAVREIHARHVHGYRGLEIPLKVFLLPCEVI
                     L"
     gene            complement(6597..7100)
                     /locus_tag="VLD60_11600"
     CDS             complement(6597..7100)
                     /locus_tag="VLD60_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45651.1"
                     /translation="MRQTNRWWCILVLILPMAIGGCDTIRPPSKSSPTDNGRFMEMWG
                     VYTHCAQSEDLDAMRVDAQRLSLAADSMGSAADPNPNESEGPVPVGPTSRLSVDPAEM
                     AASCALHAGHVAQDMGRLYVAREMFQMIVTNFPQRRYGYYTAQAREGLERLNAERSGW
                     FHQQTAF"
     gene            complement(7658..8080)
                     /locus_tag="VLD60_11605"
     CDS             complement(7658..8080)
                     /locus_tag="VLD60_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45652.1"
                     /translation="MKNPAGIISVLCLVGFLSFSHAEAASVGPLAADSSAPGAIGKLN
                     PPGLSNDVAGHAPSTLPGSSTPSNVFRDIPSISGGYSVGGATLKPYLGAGFGSGYASE
                     LDRSLNHSPSPQTDSGVRSLFGQGLTPNEFQMGIRIPF"
     gene            8631..9200
                     /locus_tag="VLD60_11610"
     CDS             8631..9200
                     /locus_tag="VLD60_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB45653.1"
                     /translation="MRKAPEFMLLGILAVASLLVQACATQSGSTAGDERTARTERIDE
                     QTIKHIPPPEFSMTTSQPRPSMRAELSARNATGLPGGSLMDALFDFDRATLREDARLV
                     LEVNAKRLQKNGATRLVLEGRGDEMGSSEYNLVLGDRRARNVKRYLQDLGLAVELKTT
                     SYGKDRPLCFQHSEDCMQRNRSVHFIVKD"
     gene            9375..9881
                     /locus_tag="VLD60_11615"
     CDS             9375..9881
                     /locus_tag="VLD60_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45654.1"
                     /translation="MADMNPWKLTSIGLVVVGITAGVTAYVMGGKNSAQDPASTQQSA
                     AQQSAARETAAKPQHKATPVQHSQPPESVVLACNKQAEDQVASKTEEMVKDAALGAAI
                     TAGVGAAAGAIADGGKGAGKGAAIGGVVGAAGGTLYGLNENKTRDERFRAAYASCLQA
                     KGYRSSTL"
     gene            complement(10009..10581)
                     /locus_tag="VLD60_11620"
     CDS             complement(10009..10581)
                     /locus_tag="VLD60_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45655.1"
                     /translation="MQTSRVLIIDDDTISLETMASTLRLRFPDAHFETAGSALASLER
                     IRSIEYAVIICDAHQPRFEGIGFVRTVRKVHPDCPVLLLLEKHNEDLMAQAMHAGAYD
                     ALVKPVEEGTLLFAVQRAVEVSRLRSEVKREEALLVERVGNMMRDLEVLYGAYGLHSH
                     FEAFMAYVEAERQRSTQKDEGPSGLGGVGQ"
     gene            10904..13036
                     /gene="glgX"
                     /locus_tag="VLD60_11625"
     CDS             10904..13036
                     /gene="glgX"
                     /locus_tag="VLD60_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSB45656.1"
                     /translation="MRIWPGHPYPLGATWDGEGVNFALFSENATAVELCLFDQANATQ
                     ETHRIRIEECTDYVWHVYLPEVRPGQYYGYRIHGLYEPTLGHRFNPSKVLFDPYAKAI
                     AGTIDWSGTLFGYRIGDPKEDLSFDDRDNAASIPKCVVVDQAFTWGGDQLLRTPWDRT
                     VIYELHVHGFTARHPDIPKNLRGTYAGLATPAVIDHLQQLGVTAVELLPVHHFIRDKH
                     LIERGLTNYWGYNSIGFFAPDIHYATSSAPAQHVWEFKTMVKALHNAGIEVILDVVYN
                     HTGEGNHLGPTLSFRGIDNASYYRLVPERPRFYQDYTGCGNTLNVRHPRVLQLIMDSL
                     RYWVLEMHVDGFRFDLASTLARELHDVDRLSAFFDIIHQDPVLSQVKLIAEPWDLGEG
                     GYQVGNFPAGWAEWNGKYRDTIRRYWKGDGGQVAELAFRLSGSSDLYEGGGRRPHASI
                     NFVTAHDGFTLHDLVSYNQKRNEANGEENRDGTNDNLSWNCGVEGPTLKPSILALRER
                     QKRNMLATLLFSQGVPMLCGGDEMGRTQHGNNNAYCQDNEISWVDWNLSKPQQALLDF
                     TRSLIALRQLHPVFRRRRFFQGRRIRGAEVKDLSWMRPDGKEMTDEDWAKGYVRCLGV
                     RLAGHAIEEKDPKGRPLLDETYLILLNAHHEPRPFTLPAHKRGVLWQPVLDTAVSHPD
                     GKLVTILRGGTRYDLEARSLAVLRLRPK"
     gene            13388..13723
                     /locus_tag="VLD60_11630"
     CDS             13388..13723
                     /locus_tag="VLD60_11630"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB45657.1"
                     /translation="MTSKDVRVLMLGMGHAMAALYMAAALTACQTTHGQTAGQYMDDA
                     AITSSVKTKLVSDRFANMTRIDVETTNGTVHLIGIVENSDQKARAGKLATQVAGVKRV
                     DNDLEIQQQ"
     gene            complement(14319..15068)
                     /locus_tag="VLD60_11635"
     CDS             complement(14319..15068)
                     /locus_tag="VLD60_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB45658.1"
                     /translation="MRGLTRSILTMSLLASVGTAGAADFTAICCDGGYEYRHPNAYER
                     EQQRLYDAIAAGRSSDTGKAAAELAVANRRIADLEKQLADRDQEMAALRAEQSAELAK
                     LKVAERGVVKGLRPQIEKGDITVDVNSERLLINLASSYLFGSGEDQLRPEGAEALKHV
                     GSVLKNFPQYKVAVDGHTDNVPIHGALMARFPTNKELSEARAISAAKALAEGGRSDAM
                     TTGYGSANPVEPNSTAAGRARNRRVEVRVTR"
     gene            15359..>16404
                     /locus_tag="VLD60_11640"
     CDS             15359..>16404
                     /locus_tag="VLD60_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB45659.1"
                     /translation="MYHLLIAALLLLTATEPAHSSQRQDIFRHAKAATVLIVGVNDAA
                     HNLSLGSGFFIDASGLVVTNAHVIEESGRLYVYVLDREIYAAPEVVAVNPDLDLAVLR
                     IPNTWTDPLALAADPTPEGTEVIAVGYPRITDILQMGFALHASIIPAMVSGLVQGQSR
                     TNGRVVDFLQTAGLLNFGNSGGPLVRTDSGEVAAMVVTTVPYLERAKDSQGVAIGSVM
                     MKSGISYSIPAPVIRQWLGANHLSPSPPPPAGAQHPLPKVAEPEADRSFATGYLLQTI
                     AMVLHGDADLLKLAIRHYNAAAQLRPDAPLIVRNLGLAYASLEQWHEAVQAYTKAVAL
                     APNDPELLIDAAMA"
BASE COUNT         3722 a         4928 c         4575 g         3179 t
ORIGIN      
        1 tatacgtcct gcaatatcga gtatgcgatc cccaccgacc aggtgatggc ggacgccaag
       61 accgtcgaag acgatcgcta caaattcgcc gaatacatca gcgaggacgc taaagcctat
      121 ctggccgaac ggtacggcta caaagaattt gtccagaagc cggcactagc ggtcacacag
      181 gcgtaatcac atgggcagaa gattcgggcg atggcccaca cgcatggccc ggatcttcca
      241 cccgtctgcc gacaggcgct gtcggcgaat cgacgcgagg ggaatcatga cagatgtgaa
      301 accgacgctc acacgttcgc cgcactatac gctgagctat ggcatgcggt tggataaggg
      361 gaccgggtca gaactgagcc acccgcacgt gcccatcatc ctgccgaacg gatcgccagg
      421 cagcatgacg ttgcacgtga tgaacggcac gccggaggaa atcaaagcgc gattgctcga
      481 aagcgtcgac gcgttcttcg aaatctacgc cgacctctga gctcaaccat tcgtgaccga
      541 ggcgtcgtct ccgcggagga ccgttccgac gcctcacctg ggaggcaacc gaccatgcag
      601 acgctcgaaa tagatcgtcc cgacatgccc gatcttcagt tcgttctgtt ggtgacggca
      661 ctctgcacct cccgcctgac cctgctcaac atcccgcaca ctctccgcgc cgtcattttt
      721 gaccgctgtt gggtcctgat tcacgacagc cccccgcccc gcaggccgga ggagcgtgtc
      781 ttagacttgc ggccctggac cgaggtgacc gtcgaggcga tggtcgaaac catctgcgcg
      841 gcgcttaccg aggcaggcat ccaaaccttg gcctgggatc atccagccag cgagccgacg
      901 atcaccagca cacctgaagc ccaacctctg atcgaccgcc tcgatcggct ctatccttag
      961 ccgaacctgc gtagcacatc atggcgtcga aagcagacga acctcgcgtt gcggaaaggg
     1021 tatttcaatg tgcaccgcgc ggaagcgctc gatgatagcc tggttcagtt ccacttcagc
     1081 cgtcccgtag tcggcgaccg acgtccaggg tttaatcgca atcctgacgg tcgagtcgcc
     1141 cagttgcgag acgccggtga agggaggggg atctttcagc acactggggt gatgggcgag
     1201 aatctcccgg acgaccgcaa gcgcctcgtt caggttcgtc gcataggcca cgccgacctg
     1261 gagatcgagc tggcggatgg tgccgaagtt atgcaggatt tcgcccacga tcttgcggtt
     1321 gggaatgacg atgcgggact gatccggatg caggagaatc gtcgagaaga tactgatggt
     1381 caggacatcc ccgtgcacgc cgagaatcga gatgtgttca cccaccttgt agggtttggt
     1441 aaaaataatc gagagcccgg ccacgacatt gctcaagacc ccttggagcg ccaacccgac
     1501 gccgaggccg gcaacgccaa tgccggcgag cagcggcgcg atctgaactc caagctggcc
     1561 cagcgctgcc aacaatgcga acactacgat gacgatctgc acgaccttgg tcaacagcat
     1621 ccggaccggt ggctccatgt cttggcgctg caaccactgt aacgtcagtt tgcccgccca
     1681 gcgggcaaca aggacaccca ccgccatgac aatgatcgcg cccagggttt gaaacccgta
     1741 gcggacggcg tattcgataa tcaggtccat tcccggcatc gtgaaacctc cccttccgtg
     1801 tgagtactcc aatgcgcagt gtctgctccc tattttgaag acgacttctt gtcttcggac
     1861 ttggtcagca agtggggact catcagttcg attcgcagga tcggccccat gagttgcaag
     1921 ggcccttcga attgcacgaa actcggcacc tcgtcgtcca ggatccaaca ttcatactga
     1981 aaattgtccg ggagcttgcc gatggccttg ccaaccatct cgcgaatcat gccgacatcc
     2041 ggcttgaaga cgtactgcac cgccttccgt gactgttcgc cgatgcgaac ggggtgctcc
     2101 cccatgagat gcagctccag cttgataatc tcaggggccg gcgtaaaggc gagaaagttc
     2161 accgtctcgc tggtgccctt cggcaggttc agcaacatcg tcacaaagat gccgttgtac
     2221 acatccttgg gaagcgtaaa cgttccggcc agcacctcct cctttccgcc ctccgccgtt
     2281 tttgagcgaa ccttgtataa cgcagtaccg cggtcgatcg agacgtcgat ctgttccgga
     2341 aacgacgggc cgcgctggat caggtggtag cggagcatcg aaaacacatg ccgttgcgag
     2401 aacgtcactt tctcatcgtg caaagagccg tccttgaacg tgaagaccaa atggctgtcg
     2461 accagatcgc cctctttgac gacctgggtt atctcaccct ggccgatgat ctcccccgca
     2521 ctcgaccgca cgagcagaaa gccgtgggtc atgccctccg gaaaccgcac cgcaatgggc
     2581 acggcgtccg caggtgagca cgcgagacca atgatcacga agatgatcgc gacggccgtg
     2641 caggccccac gatggcccac agaagtgttc agatgacaca tcatgcggaa tgccgccttc
     2701 ctgctctcaa ccgcaccgac acagaccccg tcagcgcgga caatgaagat cgtcgcgaca
     2761 ggctaggctg cctgcgggaa gactgtcaag aataggatgg caggctgtta cgtcttcacc
     2821 gaacaaggcg tagccatgct ctctagcgtg ctgcagagcg ggcgcacgca acccgatgcg
     2881 cgctaatacc ctcgaccggt gctgccgccg ccccgactca tccgatccag ctgaagcgcg
     2941 tcgtagcgtt gcttcaagtc cggatgctcg ccgagaaagc gcttcacagc tgcatcgtcg
     3001 gcaaagacct cggggctgcg ctggcgatag gcttcgaccg tcagatcata cttcgccaga
     3061 accgtgctga gcttcgcgtt gatgtctgcg ctcatttcct tcatcttctc aggcgacggt
     3121 cgctcccctt ccttgaaccg ctcaccgccc ttgaagtagt tcgtcatcat ctcgccgatc
     3181 tcgatgcggg ccctcacgaa cttctccacc tcagccggtt ccgccgccag tccggatccc
     3241 gcgaacagca caatgcccaa cgcgataccg atcatacgat gcgcaatatt catgaccatg
     3301 attcctccag atagtaaatc acaccatcgg actgtcgaag ggcctccatc ataccatcac
     3361 agccgatcca accgaatccc gctcttgagg cacaaggaac aggcctgcta cagttgccgc
     3421 aggcaccagc attgcggaca acccaccggc gactcttcct caggcgccgg ctgaaggaga
     3481 tccttctatg gcagtggcat cggtgatgct cccgctcgga acccccgctc ccccgttttc
     3541 cttgtgcgat gtcgtgagcg ggcagatcta ttcgctcgac tcctttaccg gaaagaccgc
     3601 gttgctggtc atgttcctct gccggcactg cccctatgta cagcatgtcg aacaggagct
     3661 cgccaggatc gggcgcgact accgcgatgc cagcctgggc atcgcggcga tcagcagcaa
     3721 cgatccggcg gaatatccgg acgacgcgcc ggagcagttg aaagcccagg ccctgcatct
     3781 gggctggacg tttccgtatt gtttcgacga gacccagaac gtggcgaaag cctatcgggc
     3841 cgcctgcaca ccggactttt atctgttcga tcgggaacgg cgactggtct accgcggcca
     3901 actcgacgac agccgtcccg gcaacaacaa gcccgttacc ggtcgcgatc ttcgcgccgc
     3961 catcgatgcc acgcttgccg gcagaccggt cgacagtcgc cagcaggcca gcatcggctg
     4021 cagcatcaag tggaaacgtg gcaacgaacc ggcataaggc agtgcgagtc tcttgcccct
     4081 cttcgccgat cccgtttgtc cttggggccc tgctcatggt ggccgcagcc tgtgacgggc
     4141 cgatgcaaac ggtctccatc accgcggaag atttccggtt cgtgccggat ctcgcgcagg
     4201 tgaattcgtc ctcaccgctc acgctcacca tctacaacgc cggccgcgag atacacgaat
     4261 tcgacagtcc catcctcatg tatgccgcct cgactcattc cactgcgaag caggccagcc
     4321 ccggccttct gctcgagccg ggcaagtccg tgcagctggt catggcccct ccgccgggga
     4381 catacctcta tatctgccgg cgcaaaggcc atgcgaacat gactggcacg ctgatcgtgg
     4441 aatagaaact gtggtggatt ggaaaccggc gtcgaggggc gccggttgac ggagaaacac
     4501 ggagacgggc agaaaacaca gccgatcgag cctatcaacg caacaagtcg cgctgcagct
     4561 gttccaactg tctgccgagg tgttccaggt ctgtgcgtgt cttctccaga ctgtcctcgc
     4621 cttccggcgc gagcccctgc tcccgtccct cttcggcccg caatgcgatc acgacgcgtc
     4681 caagatcctg agtgcgctcg ttgagttcca atctgagttg ctcggtcaat tgcgagaggc
     4741 ggatcgcgag gagcttatag gccacgatca acgacgccat cccccagaac gtgatcgcac
     4801 cgatcaaccg gttggtcatc gccacagatt ccgggaccaa cggaggcgac aggtcaaacc
     4861 cgacgagcat gagaatggta gagatcgcgg ccacgaagaa cggggcatac aaaagagtgg
     4921 cccaataggt aagccccagc ggaatcacat acaactgcca gggtgtgaac cccagggcca
     4981 tctgcatgtc gatcaggaaa atgccaacaa gcaacacccc gatcgccgcc cacagaccga
     5041 tctgttgaga tctcatttag tgatcgtcaa gcgccggttg cagaccaatc gtgtcattca
     5101 acagcgcctg ccgcgtaatc gccaacccgc aggtcgcaca ggtcaaaaaa cccccgttgg
     5161 actccaaggc cgtctccaga cagcggggac aggccggcgt cccaaggaca catgcagggc
     5221 gactcgtcgt cggtacacgt gacatacagg gtacagctga atcagccgcc tccccagccg
     5281 aacgtgatga ccgcttcgtc ttggcccagt gaaccgaaac cgctgagcga gaagagtgag
     5341 gatgaacacg aggacagatt cgaaaaattt tgcccattgt acagatgtca aaaaaaatgt
     5401 caaaagattt tttcagctac gggagaacaa ccaccatcgg cgattcagcc ggcaggagcg
     5461 ccgtcagtca accaatgaga gcaccacata gggggtcagg taataggcgg ccacagaaag
     5521 cagcatgccc agcacggcgc caaacaggcc tgtgacatag agccctgcaa atggggaaag
     5581 gaacacaatg acgaccatca cgatcgcgat ggaccggtgg ctttcgtaga acgtcctgcg
     5641 gaaatcactc tgaagcgatt cggtgaagga gaggtcagag ccaaaggcca tggcgaagta
     5701 tagtggaaac gatgaggaga agcatcacgg ctgcctgttc caagccagga gcacgatgcg
     5761 aggggatcgg aaatggtgcc ggacaccgtg atcttcggcg atccgtttat gtcccgacct
     5821 cctgaatttc cctccaacga ctcacaggac aagagcgtca cttggttggt agacttggcc
     5881 gaagctgagc gtacggactt gtgagccgat cttcgtctcg taccgccgtg ggagtggaga
     5941 acccgcttcg atgaacgaag gactcagaga cctggcagga agaccatccg atcaaggacc
     6001 gaccgaccaa cacaacaacg gcccaggaga atgatcatga aaatgctgca gattgtctgt
     6061 ggggagaagc ttggagagga aatcctcgcg gtgtttaaag gcctggggat cagcggctac
     6121 acgatgattt cgggcgtggg aggcagtggt cagaccggaa cggtatccgg gaccggcgcc
     6181 tcgaccgatc gaaatacgtt cttcatggtc gccctcgaag atactcacat tcacatgaca
     6241 caggtactga acgccgtgag ggaaattcat gccaggcatg ttcacgggta tcgtggcctt
     6301 gagattccgc tcaaagtctt ccttctaccc tgcgaagtga tcctgtaacg atcgtatgcg
     6361 cggatccttg tcgacgtgtg aatggtgaag aggaaggaga cgggcaggct ggtcgtcctc
     6421 ctgctcgcga aacgcgcgcc ctcagaaggg cctcgttcga cgcgcgcatt cgaggatcaa
     6481 ccagcccgcc ctatggagag acacacggta ccgccgccca ccctacgcct gactcagatt
     6541 gcagttggaa gaaccacagg aggcaggtcc ttcatagcga cctgtgagca cagctcctag
     6601 aatgccgtct gctggtggaa ccagccggat ctctccgcgt tcagtcgctc cagcccttcc
     6661 cgggcttgag ccgtgtaata gccatagcgc cgttgcggaa agttcgtgac gatcatctga
     6721 aacatctccc gggcaacgta caggcgtccc atatcctgcg caacgtgacc ggcatgcagg
     6781 gcacaggagg cagccatctc ggctggatcc accgagaggc ggctggtcgg tcccacgggg
     6841 actggcccct cactctcgtt cgggttcgga tctgcagcgg atcccatcga gtctgcggcg
     6901 aggctcaatc gttgtgcatc gacacgcatc gcatcaaggt cttcgctttg ggcgcagtgg
     6961 gtatagacac cccacatttc cataaagcgg ccgttgtccg tgggcgatga tttagagggt
     7021 ggcctgatcg tgtcgcatcc gccgattgcc atcggcagga tcagcacgag gatacaccac
     7081 cagcgattcg tttgacgcat agctctgcgc ttttcctgcc caaatctctt atgagccacc
     7141 gtcggctccg aggcttccga cgcagagtac cccctcataa taagagggtc cacccacacc
     7201 gttacgaatt cgatagcatg ttcattcatt ttgaaacaac cttccgccct gtctcatgga
     7261 tcatcagaaa tccgtcgctg cgctcatgca accacggaaa acacgtatac catttgggat
     7321 caatgagagc cgtagctcga tgaagcggcg caaaccagga aatcagtcgc gcagggagga
     7381 gacaggacgc gcgagggata caacagtcag cggagcgtca cacccaaaca cattcagact
     7441 aggcgcaata cgtatggcag tcaagcgcta taagtcgggg ccggattgtt tagtgataca
     7501 ggcacgttgc gacgcgcgtt ggaatgagga tggggcggta tcgtgcaacc caggaagaga
     7561 agcatccagg ctgccggccc ccctgcctgg agaacgtcgc aagcagcgga cggatgtgaa
     7621 aagcacagga gaatgtcgcc gtgtgtcggc agactgctca gaatggaatg cggattccca
     7681 tctgaaattc atttggagtg aggccttgcc cgaacagact tcgaacgccg gagtccgtct
     7741 gaggggaagg gctgtgattc aaagaacggt ctaattctga ggcatagccg ctaccaaaac
     7801 ctgcaccgag atacggcttc aatgtggccc ctccaaccga gtagcctccg ctgatcgagg
     7861 gaatgtctcg aaagacgttc gatggcgtcg atgatcctgg cagcgtggaa ggtgcgtgac
     7921 ccgccacgtc gttactcagg ccgggtggat tcagcttgcc gatagccccc ggcgcggaag
     7981 aatctgcagc aaggggacca acacttgccg cttccgcatg cgagaacgat aagaagccca
     8041 ccagacacag cactgatatg atcccagccg ggttcttcat ctgccgaggt ccattcccat
     8101 attgttagtc accttacaaa agtaattttc agatttcaag atccgcacca cccgttctcc
     8161 tctgcctcat caatgagttc agtcatcgta atgtattcca tgtctcatgg tgacgacacc
     8221 agctgtggag aacatatctc caccgagtac caccgcaaca cgaaatttcc gctgcgatgc
     8281 ggagactctt tgaagctgtc cacaatatgt cgtccaggct acggcatcaa agaacgtact
     8341 ggcttggaag gtaaatgaag gaaatcttcc agcggaaaca gaaagtgcga tttatgtaca
     8401 aattccgcct tgttgcagcc cgaaaatcgg caaagtcgcc ggtctgtcct catccctcca
     8461 gtcctgcatc tcaccaacct gcgagcggcg tgggaaaacc gctgatcttt tccacagttc
     8521 cacagagccc tacgcgcgct ccccctgggc tctcatggga tattgcgagc attaatggca
     8581 ttccccttgc ttatcgtgtg aaacgacctc aatcgtagaa ggaggaaggt atgcgaaagg
     8641 caccagaatt catgcttttg ggaatactgg cggtggcttc gttgctcgta caggcctgtg
     8701 ccacccagtc cggatcgaca gccggtgacg agcgcacggc ccggacggaa cggatcgacg
     8761 agcagacgat caagcacatt ccacctccgg agttcagtat gacgacctca cagccgagac
     8821 cgtccatgcg agcggagctg tctgcccgta atgcgaccgg cctaccggga ggctcattga
     8881 tggatgcgct gttcgacttc gatcgagcta cgctccggga ggatgcgcgg ttggtgctgg
     8941 aggtcaacgc gaagcgcctc cagaaaaatg gagccacgcg cctggtgctc gaaggtcgtg
     9001 gcgacgaaat ggggtcgtcc gagtataacc tggtgctggg tgaccgtcgt gcgaggaatg
     9061 tgaagcgcta tctgcaggac ctcgggctcg cagtcgagct caagacgacg agctacggca
     9121 aggatcgtcc tctctgtttc cagcacagtg aggattgcat gcagcggaac agaagcgtcc
     9181 acttcatcgt caaagactag tccgttccgc tcagccgtga gctcttgact gaaaactcta
     9241 acaagaagcg ctgtcgagtg gaatgaagcc tacgtacaga cccttttgag ttgtgcttgc
     9301 atggacaaaa taaaacttat tgctagggtt gattgactgc agcaagtacc ccgtaacaca
     9361 agaaggagta agttatggca gacatgaatc cgtggaaatt gaccagtatc ggcctcgtgg
     9421 tcgtgggcat cacagcagga gtgacggcct atgtcatggg cggaaagaac agcgcccaag
     9481 atcccgcatc aacccaacaa agtgcggctc aacaaagtgc ggctcgtgaa acggcagcca
     9541 agccgcagca caaagccact ccagttcaac atagccagcc gccggaatcc gtcgtgctgg
     9601 cttgcaacaa acaggctgag gatcaggtcg cgagcaaaac cgaagaaatg gtgaaagatg
     9661 ccgcccttgg cgcagccatc accgccggag ttggtgccgc tgcaggagct atcgccgatg
     9721 gaggaaaagg cgccggcaaa ggagcggcga tcggcggcgt ggtcggtgca gccggaggaa
     9781 ccctctacgg cctgaatgag aataagacgc gtgacgagcg cttccgcgca gcctacgcct
     9841 cctgcctcca ggccaagggt tatcggtcca gcaccttata gtagagtaga taagagagag
     9901 aacgttgcca cggcggcccc ggtcacaacc agggccgccg tgctgtttcc aggaccgtga
     9961 atcttagtgc caggcctagg ggcaagcaac gagcaagcga tacatcctct attgccccac
    10021 tccccccaag ccgctcggcc cttcgtcctt ctgtgtcgat cgctgccgct cggcctccac
    10081 ataagccatg aacgcctcga aatgtgaatg caagccgtag gcgccataca gcacctcaag
    10141 atccctcatc atatttccca ctctttccac gagcagcgct tcttcacgct tgacctcgga
    10201 ccgtagccga gagacttcaa cggcgcgttg caccgcgaac agtaacgtgc cttcctcgac
    10261 tggcttcacc aatgcatcat aggcaccggc atgcatggcc tgcgccatga gatcctcatt
    10321 gtgtttttca agcagaagca ggaccggaca atcgggatgc accttccgca cggttcgaac
    10381 aaatccgatg ccttcgaagc ggggctggtg tgcgtcacag atgatgaccg cgtattctat
    10441 cgaccgtatc cgctcgagag acgcgagggc ggaaccagcc gtctcgaagt gcgcgtcagg
    10501 aaacctgagg cgcaatgtcg aagccatcgt ctcgagagaa atcgtatcgt catcaatgat
    10561 gagaacccga gaagtctgca tgatgaatcc agaagatcaa tatccaaccc cggtttcgag
    10621 gcgcgagact caactcgctc aatagacggt tccccccaac ttccccttca tgacgattcc
    10681 gctgaaggtc cctttctgtt aagctggtgg ctgcctgctt cccgaccggt cggaggcatc
    10741 gtgtcggcaa cagtgccgac ggccatcctt ccgtacgacg ccgagtcatg gggcagcgcg
    10801 ggagtcgtga cgtcaaccag cacacgcatc atatgctatt gatcagcgag tttcacccca
    10861 caaagacatg ccgacctcac agttctcaag gaggtgacag ccgatgagaa tctggcccgg
    10921 acatccgtat cctttgggag ccacgtggga cggcgaggga gtgaacttcg cgctgttttc
    10981 tgaaaatgcc acggcagtcg aactctgcct cttcgaccag gcgaacgcca ctcaggagac
    11041 tcaccggatt cggattgaag aatgtacgga ctatgtctgg catgtctatc tgccggaggt
    11101 acgaccgggg cagtactatg gctaccgtat ccatgggctg tatgagccaa cattggggca
    11161 tcgattcaac ccatccaagg tcttgttcga tccctatgcg aaggcgattg ccggcacgat
    11221 tgattggtcg gggacgctgt ttggatatcg aatcggtgac cccaaggagg atctctcctt
    11281 cgacgatcga gacaatgccg cctcaatacc caaatgtgtg gtcgtcgacc aggcgttcac
    11341 ctggggcgga gaccaattgt tgcgaacccc gtgggaccgg acggtgattt atgaactgca
    11401 tgtgcacggc tttacggcca gacaccccga catcccgaag aacttgcgcg gcacctatgc
    11461 aggactcgca acaccggcag tgattgatca tctgcaacaa ctgggtgtca cggccgtcga
    11521 gttgctaccg gtccatcatt tcatccgcga caaacacctg atcgagcgcg gcctcaccaa
    11581 ctactggggc tataactcaa tcgggttttt cgccccggac attcactatg ccacctcatc
    11641 agctcccgcc cagcatgtct gggaattcaa gaccatggtc aaggcgctcc acaatgccgg
    11701 catcgaagtc atcctcgacg tggtctacaa ccacacggga gaaggcaatc atctcggccc
    11761 aaccttgtcg tttcgcggaa tcgacaacgc ctcgtactac cggctggttc ccgagcggcc
    11821 gcgcttttat caggactata ccggctgcgg caacaccctg aacgtccgcc acccgcgggt
    11881 ccttcaactc atcatggaca gcctgcgcta ctgggtcctc gagatgcacg tggatggctt
    11941 ccgcttcgat ctcgcctcca cactcgcgag ggaactgcac gacgtggacc ggctcagcgc
    12001 gtttttcgac atcattcatc aggatccggt gctctcgcag gtgaaactga tcgccgaacc
    12061 gtgggactta ggcgaaggcg gctaccaggt cggcaacttt ccggccggct gggctgagtg
    12121 gaatgggaaa tatcgtgaca cgatccggcg atactggaaa ggagacggag ggcaagtcgc
    12181 ggagctggcg tttcgcctgt ccgggagcag cgatctctac gaaggaggcg gccgccggcc
    12241 ccatgcgagt atcaatttcg tgacggccca tgacggattc acgttgcacg atctggtctc
    12301 gtataaccag aagcgcaacg aagccaacgg tgaagagaac cgtgacggca cgaacgacaa
    12361 tctgagctgg aattgcggcg tagagggacc gacgctgaaa ccgtcgatct tggcgctgcg
    12421 agaacgacag aaacgcaaca tgctcgccac gttgctcttt tcgcaaggcg tcccgatgct
    12481 ctgtggcggc gatgaaatgg gacggacgca acatggcaac aacaatgcct attgccagga
    12541 caacgagatc agttgggtcg actggaattt gagcaagcct cagcaggcgc tcctggattt
    12601 cacgaggagt ctcatcgcat tgcgacagct acaccccgtc ttccggcgcc gccgattctt
    12661 tcaaggccgc cgtatccgtg gcgcggaggt caaggatctc tcctggatgc gccccgatgg
    12721 caaagagatg acggatgaag actgggccaa agggtatgtc cgctgcctgg gcgtacgact
    12781 tgccgggcat gcaatcgagg agaaagatcc caaaggcaga ccactcctgg atgaaaccta
    12841 cctcatctta ctcaatgcgc atcacgagcc acggccgttc actctgccgg cgcacaagcg
    12901 cggcgtgctg tggcaaccgg tgttggacac ggccgtatca catcctgacg gaaagctggt
    12961 cacgatcttg agaggtggaa ctcgctacga tcttgaagcc cgctcgcttg ccgtactccg
    13021 cttgcgacca aaatgagagc agatgccgcc ctgaagggta ggaacgagcg gccttagcgg
    13081 gatcatctta ataggtgctg atgccggcgg cggaggattc tgccatccca tcacgaggga
    13141 caggttgtct cgcccaggac aatcaatcct gaattcctct ctctttcttt tttaaccttc
    13201 caatcgaagc ctcaagtact agttcccaaa agcaccgcaa ccgtacgaaa tttcaagcaa
    13261 tcctttcacc taacaagaga acaaggcaag taagggcaag gtgctgatcc cctggcattc
    13321 tccttgcgga ttacgttcac gtattcctgg aggtcggacc aatcaccttc gcaagggagg
    13381 agatctcatg acttcaaagg acgtaagggt gttgatgttg ggaatggggc acgcgatggc
    13441 ggccttgtat atggctgcgg ctctcacggc ctgccagacc acgcatggtc agaccgcggg
    13501 ccagtacatg gatgatgccg cgataacctc ctctgtgaaa actaaattgg tgagcgacag
    13561 gtttgcgaat atgacacgca ttgacgtgga aaccacgaac ggaacggtcc atctgatcgg
    13621 gatcgtcgag aattccgatc agaaagcccg ggccggcaaa ctggccacac aggtggcagg
    13681 cgtcaaacgc gtggacaacg atcttgaaat ccagcagcag tagcgatcag acagaagagg
    13741 ggcctcggca tcctgccggg cccctccgaa attggcaaag attcttggaa ccatttgctg
    13801 cgcgaagcta tccctgcagc atggcgcgag ccgatcagaa aaagttcctc actccccatt
    13861 tcgacaagcg agaattggaa gatcagcgac gcggccgatc gatacgccga ttgccgctcc
    13921 ttttcagata tttattacca ctgaccccct gaaagggagg aaggctgtcg tgtcgatcaa
    13981 gaaagaggtc caacagttta tcattgcatg tgaaaaactc catcggctcc tgtccagagg
    14041 ggaacacctc acgtgtcatg aagccgaaat cctgcagtgc tgcatcgacg agctcaccaa
    14101 acggcacgtc acgaatcctc cccgccagcc aaacgattcc aacgaatcgg cgcgcccaca
    14161 ctgatgggaa aaaacgtaac ctgtcgatct cctgtacccg cgcaacggat ggtctcaaaa
    14221 aattggggag gggccagcta gcctggtccc tcccctctga ctttgccaac acctcttatt
    14281 ccaaacggga aaacgagaat gtcccggctt cgggtgtcct acctcgtgac gcgcacctcc
    14341 acgcggcggt tcctggcccg acccgcagcg gtgctgttcg gttcgaccgg attggcggat
    14401 ccatacccgg tcgtcatcgc atcgctacgc ccaccctcgg ccagcgcctt cgctgccgag
    14461 atcgcacgag cctccgacaa ttccttgttt gtcggaaacc gagccatgag ggcaccatgg
    14521 atcggcacgt tgtcggtgtg accgtccacg gccaccttgt actgagggaa attcttcaag
    14581 accgatccaa cgtgcttcag cgcctcagca ccttccggcc tcagttgatc ttcgccggaa
    14641 ccgaacagat aactggaggc caggttgatc aagaggcgct cgctgttcac atcgaccgtg
    14701 atgtcgccct tctcgatctg aggacgaagg cccttgacca ctccgcgctc cgcgactttc
    14761 agtttcgcca actcggcaga ctgttcagcc cggagggcgg ccatctcctg gtcgcgatca
    14821 gccaactgct tttcgaggtc ggcaatgcga cgattggcca cggccaactc ggccgcagcc
    14881 ttgcccgtgt cgctcgacct gcctgccgct atggcatcat acagacgttg ctgttctctt
    14941 tcgtaagcgt tcggatgacg gtattcataa ccgccgtcgc aacagatggc cgtgaagtcg
    15001 gcggctcctg ccgtgccgac cgacgccagc aggctcatgg tcagaatcga tcgtgtgagt
    15061 cccctcatga tctctccctt cattcggcca aatggtcact caacatgcca ttaagcaaaa
    15121 actatggcca gggctggtgg tctggggatg gatcgcacat ctgcttgcga attctaaagg
    15181 ttacgtactt atccatttga tgacgctggc gtagaggcaa tgatgattgt ggaaaaaggg
    15241 ggcaagggtc gggaggatat gagggtagaa tgcctcaccc agcagacacc tcaagaaagg
    15301 gggaacagca tttgacgtag tagttgacca gcctctctgc tctgctagac tccacactat
    15361 gtatcatctg ctgattgcag cgttgcttct cttgactgcc acggagccag cccattcgag
    15421 ccagcgacaa gacatcttcc gccacgccaa agctgcgacc gtgctcatcg tcggtgttaa
    15481 cgatgcggct cataaccttt ccctgggaag cgggttcttc atagatgcca gtggcttagt
    15541 ggttacgaat gcccatgtga tcgaagaaag tggccgcctg tacgtgtatg tcctcgatcg
    15601 agagatctat gctgccccgg aggtcgtggc cgtaaaccct gatttggatc tggcagtcct
    15661 ccgcatcccg aacacgtgga ccgaccccct cgcgctggca gcggacccta caccagaagg
    15721 gactgaagtg atcgccgtcg gctatccccg gatcacggat atcctccaaa tgggttttgc
    15781 gctgcatgcc tcgatcatcc cggcgatggt cagcggcctc gttcaaggac agtcgaggac
    15841 gaacgggcga gttgtcgact tccttcaaac ggccggtctt ctgaacttcg ggaacagcgg
    15901 agggccgctt gtgcggacag actccggcga agtggctgcc atggtggtta cgaccgtccc
    15961 ctacctggaa cgagcgaaag actctcaggg tgtcgcgatc ggaagcgtca tgatgaaatc
    16021 gggcatcagt tactcgatcc cggctccggt gatccggcaa tggcttggcg cgaatcacct
    16081 gtcgccatcg ccgcctccgc cggccggagc ccaacacccg ctccccaaag tagctgagcc
    16141 cgaagccgac cgttccttcg ccaccgggta tctgttgcag accatcgcga tggtacttca
    16201 tggggatgcc gatttgttga agcttgccat ccgccactac aatgcggcag cccaactgcg
    16261 gccggacgcc cccttgatcg tccggaacct cggcctggcc tatgcatctc tggaacaatg
    16321 gcatgaggcc gtgcaagcct acaccaaggc cgtcgcgctg gctccgaacg acccggagct
    16381 actcatcgac gcagccatgg caca
//
LOCUS       DATIFR010000201         8796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1668441, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000201 DATIFR010000000
VERSION     DATIFR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668441"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1112
                     /locus_tag="VLD60_11645"
     CDS             168..1112
                     /locus_tag="VLD60_11645"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSB45660.1"
                     /translation="MTIAAQFPDEQSEAGEFTRQADAFREYVTASGSSRYPAEAGRYH
                     LYVSWACPWAHRTIIVRKLKNLEGVIGMTVVDPIRDERGWAFREGSGHSLDPINGFHF
                     LSEAYKATDPNYIGRFTVPVLWDRLTRRIVSNSDDDLMRMFNGEFNRFTDSTIDLYPE
                     GLRREIDDLNVFIYENVNDGVYRAGFATSQPAYERAVRRLFEALDLLESRLAHHRYLF
                     GSQFVETDWRLFVTLIRFDAVYHGHFKCNLRRIVDYQNLFGYLKDLYQTDGIADSVNF
                     DHIKRHYYITHDDINPTRIVPLGPDQDLTSPHGRERLL"
     gene            1280..2125
                     /locus_tag="VLD60_11650"
     CDS             1280..2125
                     /locus_tag="VLD60_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45661.1"
                     /translation="MSVDCRVCAAILYALVGLLPAHLADDAPAAAFSQPSLVTFSAWD
                     HSFEGPDVIPAGQTTIHLHNGGREPHQLQLLKLEGGKTPADLEAILQEGDGTIPSWAK
                     HVGGPNGIGSGGDAQATIYLEPGSYVLICLIPTKNQQTHAALGMQKALRVTESTPSPP
                     EFYGNFHMAMFDYEFVVVQPLKKGQHTFYVINRGNQRHQVSLVRLDPGSSTDEVLAAF
                     GSNGAAALPGQMIGGMSGLEPGREGMFSTDLAPGRYAMICLFPNPTAHDSHAAKGMVM
                     NFTIE"
     gene            2394..2879
                     /locus_tag="VLD60_11655"
     CDS             2394..2879
                     /locus_tag="VLD60_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSB45662.1"
                     /translation="MVILKRLQSYLDGRKIPYQVVNHSVAYTAREAAESLHVPADTFA
                     KVVIVKADGRYVMAVLPSTWKVDFKRLEEVLGCPHVRLATEDELAVLFPDCEIGSMPP
                     FGNLYGTPVYVDATLTQDEAIVFDAGSHVGAMKMRYRDFADLVHPQVAEFHREPSKLE
                     Y"
     gene            3149..4150
                     /locus_tag="VLD60_11660"
     CDS             3149..4150
                     /locus_tag="VLD60_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45663.1"
                     /translation="MFHEFSSTVRALLRDAAGSLYHIVVVALSAGIALLLPAGARHFL
                     SFWSRVEHDKFSLIAVEMAVAILLIAGLSYVHRSMRDRTLAAAATGAGLVSFFPRRAR
                     GAERRIRKLKEEQGTGRTIMVMGSSGAGTFGDQVGDLSSVLDKCIGANILLVNPYSQE
                     ASQRIQAIDHPKYTLAAFRDEVRESIALLKRLKAMGKTVKLKLYADPPLVKMVILGDY
                     LWLQHYHADVDVHTMPEYVLQHNRKAHGLYSLCTHYFEQRWASTEIPEYDLETDELVY
                     RSRTGSEIRKEPFECEPAPEIAGTIVIAPDLVPEPVHSSQARTFQAQQRRPIAPMNR"
     gene            complement(4256..5041)
                     /locus_tag="VLD60_11665"
     CDS             complement(4256..5041)
                     /locus_tag="VLD60_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HSB45664.1"
                     /translation="MKRVTIRIVRALVLLMVGLLAQPSFALDHDNLDPNRPIGMEDAY
                     AIPKGEIGVEGGVRFNDQRQGPTRLTFQPQIIYGAFDNTQIEIQGDIFTDPNTIVGPN
                     KSGDLHLGVLYNFNTETLDLPAFAVRAEVDLPTGVNSQGVDTQVTGILTRSFGRLRAH
                     LNAGYTILGSPQGQERPGAYRAVAAVSYPLGYPTSFRDTLIASVYTRQSDQRGQRNNT
                     GVEIGLRHQLTSRIVLDGGLGTEFYGPSDRAALLGTVGVSVGF"
     gene            complement(5038..5985)
                     /locus_tag="VLD60_11670"
     CDS             complement(5038..5985)
                     /locus_tag="VLD60_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45665.1"
                     /translation="MRINKCIRVAAIVATATVALALAACSQFEPRDKRFYYRALWNFA
                     LREDLAELDSEFNGVDFGHSNLYENLLLTGGNDIPAIDDRARKETLAFIASKPRLNPN
                     EEAIAPTYMKLAWRAQNTFDEAHALHRATYDIVVSDEPDKDRALRNVLAYYQESAYAI
                     TAKRLDHHRLDQFPYSKTFRTRFPLFNATIWSYHYLQVAVYDPLQAAPDLDAKKQAVR
                     PILATYRGYLEQPPVRWTFMPLTAELSPAFAARYPEIANIFDNLHMLHDNISDILASE
                     QLPTWDAKRAEIYRVLDHYYLASADETNPMIVKVQEHHH"
     gene            complement(6014..6610)
                     /locus_tag="VLD60_11675"
     CDS             complement(6014..6610)
                     /locus_tag="VLD60_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HSB45666.1"
                     /translation="MMHIESATATLPEEGEPQICCAKPKPQDRQYTKHEQHGHQVHHD
                     HHEGAHKAASLNRTAFKATLHCLTGCTIGEVLGMVIGTALGWTNWPTVALAVTLAFLF
                     GYGMTLWPLRRAGMAWGAALGLAFASDTLSMTTMELVDNAIMIVIPGAMDAGLLDPLF
                     WGSLLVSLILAGVAAFPVNRWLIAKGKGHALVHEHHCH"
     gene            complement(6747..7385)
                     /locus_tag="VLD60_11680"
     CDS             complement(6747..7385)
                     /locus_tag="VLD60_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB45667.1"
                     /translation="MTETILVKDNAPMPSTKPSLLSNPEENPTNPLLHDLTHRLLEQQ
                     SAFRAFLQKRLSDDGVVEDLLQHSLMKAVERGHELKKSESVVGWFYRILRNVVVDYYR
                     THAADNRKVEGLLREMVSSGEDKTPALDEIRPAVCACLGALLKEIRPTYADLIRRIDL
                     EGESPAALAKDLGVTPNNLTVRLHRARQALKAKLEQACGICTKHGCLNCTCS"
     gene            complement(7792..8379)
                     /locus_tag="VLD60_11685"
     CDS             complement(7792..8379)
                     /locus_tag="VLD60_11685"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSB45668.1"
                     /translation="MGKRIAIIHGHPDASTRHFGHALADEYAKGCEDGGHEVKRIEVA
                     TLDFPLLRTKEDFEKGTPPDSIAQAQDAIKWADHLVIIYPLWLGSMPALLKAFLEQVL
                     RPGFAFEYQKSGGIAKKLLTGRSARIVVTMGMPAFVYRWIFFAHSLKSLRHNTLWFCG
                     IGPIRSTIIGSIEGMSEKQRLGWLDEMRGLGELGK"
BASE COUNT         1828 a         2586 c         2469 g         1913 t
ORIGIN      
        1 tcagcgcagt ttttccatga aaaccaagcc gtccgtagcc ttttgccacg gtattaaatc
       61 agtaccgtgg tgaaacgcaa cagcgccggg ctggcagtac aacactcgtt gggtcatttt
      121 ctctatcgat tatgagatac tagtcaaata agaggccccg tccctctatg acaattgcgg
      181 cacagtttcc tgacgagcaa tcagaagccg gagaattcac acgacaggcc gatgcctttc
      241 gcgagtatgt gacggccagt ggaagctccc gctacccggc agaagccggc cgctaccatc
      301 tctatgtctc gtgggcctgc ccatgggcgc atcgcaccat tatcgtgcgc aagctcaaga
      361 acctcgaagg cgtcatcggc atgaccgtcg tcgatccgat tcgtgacgag cgaggctggg
      421 cattccgtga aggctccggt cactcgctcg atcccatcaa tggatttcac tttctcagcg
      481 aagcctacaa agccaccgac ccgaactaca tcggccgctt cacagttccg gtcctgtggg
      541 accgcctgac caggcggatt gtcagtaact ccgacgacga tctcatgcgc atgttcaacg
      601 gcgagttcaa tcgattcacc gacagcacga tcgatttata tccggaaggg ctccgaaggg
      661 agatcgacga cctcaatgtc ttcatctatg agaacgtgaa cgacggggtc tatcgtgcgg
      721 gctttgccac atcccagccg gcctatgaac gagctgtgcg gcggctcttc gaggccttgg
      781 atcttctgga gtcccggctc gcgcaccacc gctacctctt cggatcgcaa ttcgtcgaga
      841 ccgactggcg cctgttcgtc acgctgatcc ggttcgatgc ggtctaccat ggccacttca
      901 aatgcaacct ccgtcggatc gtcgactacc aaaatctctt cgggtatctc aaagaccttt
      961 atcaaaccga cgggatcgcc gacagcgtta acttcgatca catcaagcgg cattattaca
     1021 tcacgcacga cgacatcaat cctacccgga tagtccccct gggtccggac caggatctca
     1081 cctcgcccca cgggcgcgag cgactattgt agggaccatt cggctcttcg gcttcagcca
     1141 aacggatctg tcgacattcc ctaagtattt gtcgagtatt gcgaaggagt tcatgtcaga
     1201 gtataaggag ggccgtgcgg ccggtccgtg gcacgacttt gtgatggaag gtcggggaac
     1261 tcaatcccgg gaggacgcta tgagtgtgga ttgccgtgtg tgtgcggcta ttctgtacgc
     1321 tctcgtcggg ctcttgccag ctcaccttgc tgatgatgct cccgccgctg ccttttccca
     1381 gccaagcctg gtcacgtttt cagcctggga ccatagcttt gaaggtccgg atgtcatccc
     1441 ggcaggccaa acgacaatcc acctccacaa cggcgggcga gaaccgcacc agctccagct
     1501 tctcaaattg gagggaggga aaactcctgc cgacttggaa gccatcctcc aagaaggcga
     1561 cggaaccatc ccctcgtggg ccaagcatgt cgggggacca aatggaatcg gatcaggcgg
     1621 tgatgcacaa gcaaccatct atctggagcc aggctcctac gtccttattt gtctgattcc
     1681 caccaagaac caacaaacgc acgcggcgct ggggatgcaa aaagccctgc gcgtgacgga
     1741 gagtaccccc tcgccgcctg agttttacgg caacttccat atggccatgt tcgattatga
     1801 atttgtcgtc gtccaacctc tcaagaaagg ccaacacacc ttctatgtca ttaatcgagg
     1861 gaatcaacgg catcaggtga gtcttgtccg gctcgatccg ggctcctcga ccgatgaggt
     1921 gctcgccgca tttggatcga acggtgctgc tgccttgccg ggacaaatga tcggtgggat
     1981 gagcgggctg gaaccgggca gagaaggaat gtttagcaca gatctggctc caggacgcta
     2041 tgccatgatc tgtctgttcc cgaatcccac ggctcatgac tctcacgcgg ccaaaggcat
     2101 ggtcatgaac ttcaccatcg aataactcta cccggctgcg agcggggcag tcgacggaat
     2161 actcagccgg gtcaaccgtg gcgattcagg acaaatacgt ttcgaactct tgcccctttc
     2221 tgccacgggt tcgtaagaaa cagtgaccca gccatctttc gctctccgct aacccggctt
     2281 ctcgattgat ctttgcttct tgtgcttcgc atcatgtcat ggcactttct ttgcgtgctc
     2341 ttccctggat aagaagagcc gtcttccaac atccgccgca tcgggaggac actatggtta
     2401 tcctcaagag gctccaaagc tatctcgacg gccgcaagat cccctatcag gtggtgaacc
     2461 attcggtggc ctatactgcg cgggaggcag cagaaagttt gcatgtcccc gccgatacgt
     2521 ttgcgaaggt cgtcatcgtc aaagccgatg gacgctatgt gatggccgtt cttccctcta
     2581 cctggaaagt cgacttcaag cgattagaag aggtgctggg ctgtccacac gtgcggcttg
     2641 cgacggaaga cgagttggcc gttctgtttc ctgattgcga aatcgggagc atgccgccgt
     2701 tcggcaatct ctacggcacg ccggtctatg tcgatgcgac gctgacacag gatgaagcca
     2761 tcgtgttcga cgccggcagc catgtggggg ccatgaagat gcggtatcgg gatttcgccg
     2821 atctggttca tccgcaggtg gctgaattcc atcgcgagcc gtcgaagctc gaatactaaa
     2881 atcagtgttc ggccgcccat gcgtctcttg tgagataggc ttgcgtcctc tacccaatcg
     2941 tgttaggctg agtctcgatc gcgtaagacc ttccggttcg ggggctgcag tcccccggta
     3001 gagaccgagt ctgacgcgat tttttttgtt cccatgccac cgtcccgcat ctgctgagca
     3061 caccccaaag gcattaccgc ccatcgctcg catgaccagc tcgacagagt cgtgaggact
     3121 gtttctcacc atttcaagag gtgtttcgtt gtttcatgaa ttttcgtcaa ccgttcgagc
     3181 actgctccgc gatgccgcgg gaagtctcta tcacatcgtc gtcgtcgccc tgagtgcagg
     3241 cattgcactc ttgctcccgg caggcgccag gcatttcctg tcgttctggt cgcgggtgga
     3301 gcacgacaaa ttttcgctca tcgccgtgga aatggccgtg gccattttgc tgatcgccgg
     3361 cctcagctat gttcatcgca gcatgcggga ccgcaccctc gccgccgcgg ctaccggagc
     3421 cgggctcgtg tcgttcttcc cccggcgcgc tcgaggcgca gagcggcgca tcagaaaact
     3481 caaagaggag cagggaaccg gacgcaccat catggtgatg ggatcgagcg gcgccggcac
     3541 ctttggcgac caagtcggcg atctctcctc ggtcctcgac aaatgcatcg gcgcaaacat
     3601 tcttctggtg aacccctaca gccaggaggc cagccaacgc attcaagcca ttgatcaccc
     3661 caaatacaca ctggctgcat tccgtgatga agtccgggag agcattgcgt tactcaagcg
     3721 gctcaaggcc atgggaaaaa ccgtgaagct caaactgtac gccgatccgc cgctggtcaa
     3781 gatggtcatc ctgggagatt atctctggct ccagcactac catgctgatg tggatgttca
     3841 caccatgccg gagtatgtct tgcagcacaa ccgcaaggct cacgggctct actccctctg
     3901 cacccactac ttcgaacagc gttgggcaag tacggagatt cctgaatacg atctggagac
     3961 ggacgagctg gtgtatcgaa gcagaaccgg cagcgagatc cggaaggagc cgtttgagtg
     4021 tgagcctgcg ccagaaatcg cgggaaccat cgtgatagcg ccggatctcg ttcccgaacc
     4081 ggttcactcg tctcaggcac gcacgtttca agctcagcaa agacggccaa tcgcgccaat
     4141 gaaccggtga gtacaaaatt aggcgaggcc ggcaacgtat gctgctcacc gttcaagccg
     4201 cttcatacgt aacgaccgcg caagcggtcg agtccgctag gccaggggcc tgcgctcaga
     4261 atccgacaga taccccgaca gtgccgagca aagctgcgcg atcggatggc ccatagaatt
     4321 cggtgccgag accgccatcg aggacgatgc gcgaggtgag ttgatggcgc aggccgattt
     4381 cgacgcccgt gttattacgt tgtccgcgct gatccgattg tctcgtgtag acgctggcga
     4441 tgagcgtatc gcggaaactg gtgggataac ccaatggata actgaccgct gcgacggcac
     4501 gataggcgcc tggtctttct tgtccctgcg gtgagccgag gatcgtatag cccgcgttga
     4561 gatgagcgcg aagcctaccg aacgagcgcg tgagaatgcc ggtgacctgc gtatccaccc
     4621 cttgtgaatt caccccagtc ggcagatcga cctcagcccg aacggcgaag gccgggaggt
     4681 ccagtgtttc ggtattgaag ttgtacagga cgcccaggtg caggtccccg gatttattgg
     4741 gccccacgat cgtgttgggg tccgtaaaaa tatcgccctg gatttcgatc tgcgtgttgt
     4801 cgaacgcgcc gtaaatgatt tgcggttgaa acgtaaggcg cgtaggacct tgccgctgat
     4861 cgttgaaacg cacaccgcct tccaccccaa tttcgccttt cggaatcgca taggcatctt
     4921 ccatcccgat cggccggttc ggatctagat tgtcatggtc cagcgcgaag ctcggctgtg
     4981 ccagcagccc gaccatcagc aacacaagcg ctctcactat acgaatagta acccgcttca
     5041 atgatggtgc tcctgcactt tgacgatcat cggattggtc tcgtcagcgc ttgccagata
     5101 gtagtggtcc aacacccgat agatctcggc ccgcttcgcg tcccacgtcg gcagttgttc
     5161 gctcgcaaga atgtcgctga tgttgtcgtg cagcatgtgg aggttgtcaa agatgttggc
     5221 gatctccgga tagcgcgcgg cgaatgcggg actgagctcc gctgtgagcg gcatgaaggt
     5281 ccagcgcacg ggcggctgct cgagatagcc gcgatacgtg gcgagaatcg gccgcacagc
     5341 ctgtttcttg gcgtcgagat cgggcgcggc ttgcagcgga tcgtacacgg cgacttggag
     5401 gtagtgatac gaccagatcg tggcgttgaa gagggggaac cttgtgcgga acgtcttgga
     5461 gtagggaaat tgatcgagac ggtgatggtc cagccgcttg gccgtaatgg catacgcgct
     5521 ctcctgataa taggccagaa cgttccggag cgcgcggtct ttgtctggtt cgtctgaaac
     5581 gacgatgtcg taggtggcgc ggtgtaacgc atgcgcctcg tcgaaggtgt tctgcgcgcg
     5641 ccaggccagc ttcatgtacg tcggggcgat ggcctcctcg ttgggattga gtcggggttt
     5701 gctggcgatg aaggcgagcg tctctttgcg cgctcgatcg tcgatggccg ggatatcgtt
     5761 ccctccggtg agcagaaggt tttcgtacaa attcgagtgg ccaaagtcga cgccgttgaa
     5821 ctcgctgtcg agttcggcca gatcttcgcg cagcgcgaag ttccacagag cgcggtagta
     5881 gaagcgcttg tcgcgcggtt cgaactgact gcaggcggcc agggcgaggg cgaccgtggc
     5941 cgtcgccacg atcgccgcaa cacggatgca tttattgatc ctcatgctgt ccatacgtcc
     6001 atcgggatct gatttagtgg cagtgatgtt cgtgtaccag tgcatggccc ttacccttcg
     6061 cgatcaacca gcgattcacc ggaaacgccg cgactccagc caggatcaag gagaccagaa
     6121 ggctccccca gaacagcgga tcaagcaatc cggcatccat tgcgccaggg attacgatca
     6181 tgatcgcatt gtccacaagt tccatcgtcg tcatggacaa ggtatcggat gcgaaagcca
     6241 gcccgagcgc ggcgccccag gccatgccgg cccggcgaag cggccagagc gtcatcccgt
     6301 agccgaacag aaacgcgagc gtaacggcca gcgccaccgt cggccagttc gtccagccga
     6361 gcgccgtgcc gatcaccatg ccgagaactt cgccgatggt gcagccagtc aagcagtgca
     6421 aggtggcctt gaaggcggtg cggttgagcg acgcggcctt atgcgcgcct tcgtggtggt
     6481 cgtgatggac ttggtgacca tgctgctcgt gtttcgtata ttgacgatcc tgcggcttcg
     6541 gcttggcgca gcagatttgc ggctcccctt cctccggcag cgtggcagta gcggattcga
     6601 tgtgcatcat gtgatacctc cttgtgaggg gtgaatgttg ccgttcaccc ctacagacgg
     6661 aggagaagcc ggattattac agcggctcgt aagtctcgat cttataaagg ggaaatgccc
     6721 tcggtcggaa tcacgtctgg tccgcgctac gaacaggtgc agttgagaca gccgtgtttg
     6781 gtgcaaattc cgcaggcctg ttccagcttc gctttcagcg cctggcgcgc acggtgaagg
     6841 cggacggtga gattgttcgg cgtcacacct agatctttgg ccagcgcagc aggtgattct
     6901 ccttctagat caatccgccg aatcaagtcc gcatatgtcg gtcggatctc cttgaggagt
     6961 gcccctagac aggcgcacac ggcgggccgg atttcgtcta acgcaggcgt cttgtcctcg
     7021 ccggaggaga ccatctcacg gagcagaccc tcgacttttc gattgtcggc agcgtgggtg
     7081 cggtagtagt ccaccacgac atttcgcaaa atgcgataga accaaccgac gacgctctcg
     7141 ctcttcttca attcatggcc ccgttcgacg gccttcatca aactgtgttg cagcagatcc
     7201 tccacgacgc cgtcatcgga caggcgtttc tgcaggaatg cccgaaaggc ggactgttgc
     7261 tccaacaagc gatgggtgag atcgtgcagc agcggattgg tgggattctc ttcgggattg
     7321 ctcaacaatg acggcttcgt gctgggcata ggagcattgt ctttcaccag aatggtctcg
     7381 gtcaaatcct gtcatgctag gcaagagacc gcgaggtcat acatcagtac gtcggcccat
     7441 aatggctctg gctttaatgg acacagtcag cctctattcc tcctcgtcgt cagccatact
     7501 attttcccaa ctcgcctaat cctcccatct catccagcct cgaatgctcc ctccaagctt
     7561 gctgcgtcct gccttctccc gaagcacggg acggatggaa ccgatgcgcg gtgaggcccg
     7621 tgtcatagcg gacgctgcca atgaggcagc tgaagtggtg ccgaatgcga cggcattgcg
     7681 atgcttcatc gcagctttat agtcgcatac ggccaacagt aacaaaacgt tttggcgtca
     7741 ctggtcaacg cattatgacg cgaccgaacc gcgcaccgtt cgtcagccac attatttccc
     7801 taactcgccc aatccccgca tttcatccag ccatcctaac cgctgttttt cgctcatccc
     7861 ttcgatcgaa ccgatgatcg tcgatcggat cggcccgatg ccgcagaacc agagcgtgtt
     7921 atgccggaga ctcttgaggc tgtgggcaaa gaaaatccag cggtagacga aggccggcat
     7981 gcccatcgtg acgacgatgc gggcggacct gccggtgagc aactttttcg cgatcccgcc
     8041 ggacttctga tattcgaacg caaagcccgg gcgcaacacc tgttcgagaa atgctttgag
     8101 caacgcaggc atcgacccga gccagagggg atagatgatg acgagatggt ctgcccactt
     8161 gatcgcgtcc tgcgcctgtg cgatcgaatc cggcggcgta cccttctcaa aatcttcctt
     8221 ggttcgcagg agaggaaagt cgagcgtcgc gacctcgatc cgcttcactt cgtgcccgcc
     8281 gtcctcgcag cccttcgcat attcgtcggc cagggcatgc ccaaagtgcc gggtggatgc
     8341 gtcaggatgg ccatggatga tcgcgattcg tttgcccatc cgcccctcgc tttcgtcttg
     8401 cttaccctgc tcgcggcatg ctcccttctt cgcattgtgc aagttctcaa ccacgactta
     8461 cacagagaac attgccccgg tttcgtggcg tttcagggca gatgacatcg cgtcattgcc
     8521 ccataatgcc acgtgaacgg gtccgccact gcttctcgca ccaacagcct tagacaatct
     8581 cctttgtgtg accagcgttt gcaaaatgct caacatgaac gaagcatggc attttccttg
     8641 ctcacttcaa tcggtaaacg aacgagatac acaacgcctt ttgtccatcg agggaggttg
     8701 ccatgccgct cctgaaacgg ctccaaagtt atctcgacac gaacaagata ccctatgaag
     8761 tggtcagtca tccgaaagcc tacaccgccc atgacg
//
LOCUS       DATIFR010000202        27671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1478510, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000202 DATIFR010000000
VERSION     DATIFR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478510"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VLD60_11690"
     CDS             complement(<1..411)
                     /locus_tag="VLD60_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB45669.1"
                     /translation="MYLKSLEMLGFKSFAEARIEFPNGVTAIVGPNGSGKSNVVDAIL
                     WVLGEQSTKTLRSEKMEDVIFNGTEVRKPLGMAEVSLVISGLDQLKVDSQSGLPSQLS
                     EYQDLMITRRLYRNGDSEYLINKTLCRLKDVRSVL"
     gene            complement(583..1545)
                     /gene="ispH"
                     /locus_tag="VLD60_11695"
                     /gene_synonym="lytB"
     CDS             complement(583..1545)
                     /gene="ispH"
                     /locus_tag="VLD60_11695"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSB45670.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRQKGAVFVEELSEVPEGSVVIFSAHGVAKSVWEEANRRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYDLILIGHAGHPEVIGTLGQIPDKFHLVSSVEDVEMLQVENRTNLSY
                     VTQTTLSVDECRDIVGALHKRFPHIKGPHQEDICYATQNRQNAVKELSRLVDVILVIG
                     SPNSSNSNRLRELGEHCGIDSYLIDSAADINPAWLKNVKAIGITAGASAPEVLVEEVV
                     AYLKTYGTAEVEDLTVIEEDVEFLLPKELITIESSGKSVAASAG"
     gene            1699..2409
                     /locus_tag="VLD60_11700"
     CDS             1699..2409
                     /locus_tag="VLD60_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45671.1"
                     /translation="MASAPAKKILIVEDERDILHLVKLYLEKEGFRTITAATGAEGLN
                     SAKCDKPDLIVLDLMLPEIDGLEVCKRLRSAPETAMLPIIMLTAKAEESDTVIGLELG
                     ADDYVTKPFSPKALVARVKALFRRLDRSQQDNLTVFRYGPLTMDLSRHEVTVRGKDVS
                     LTAKEFGLLEHLLRNPGRVLTREVLLNHVWGYDYFGTTRTVDVHVRRLKQKISLLDDA
                     ILSVKSLGYKLKDPTGNA"
     gene            2502..4310
                     /locus_tag="VLD60_11705"
     CDS             2502..4310
                     /locus_tag="VLD60_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45672.1"
                     /translation="MRLSIRWKVTLATLFALVCGFAIAGFLATRSLEEQELAQSNQTL
                     EIRTNLIAFELRPLLPTLPATPHLQIMVRELSQRALARVTLIDDTGRVLADSAVQDRD
                     LGTVENHRTRPEIEQAVATGFGTDLRASHTTGERTLYRAIRLSGPAETSSPFYLRLGL
                     PLTTLDLELAKLRRNLTLAFVSAFLIAVGLSIWLAGSLTKPLLDMAAVARQLAAGNHT
                     VHIRTSARDEVGLLADTLNHMTDELKTRIEELSEDRSQLLAMLTSMVEGVMVLDYRGR
                     VLQVNPALERMFGVTRAETRGRLFSEVFGHPELNSLVSTVLATRAGQEDEIILAPSGR
                     CLHIEASVAGGEQENEACAVLVFHDITELRRLEKIRKDFVANVSHELRTPLTSIKGYV
                     EALLDGGKDDPDTSVRFLDIILKQSDRLNLILEDLLQLSKIESGQVQFKREPLHIGTV
                     VERTIAMIKPLADKKQHQLIAQVSSDLPLVTGDQERLVQVLANLVDNAIKYTPEGGQI
                     TVAARRIPAAKSGTAAQQDGIELSVADTGIGIPEQDRPRVFERFYRVDKARSRELGGT
                     GLGLAIVKHIVEGHGGQVWVERNLPTGSRFVVRLPA"
     gene            complement(4327..5319)
                     /locus_tag="VLD60_11710"
     CDS             complement(4327..5319)
                     /locus_tag="VLD60_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSB45673.1"
                     /translation="MPELTGMLLVVVVLALLFDFSNGWHDSANAIATVVSTRVVSPLI
                     AVMGAGLLNVAGAFMSTAVAKMVGSGIVDPTSVTQQMVAAALAGAICWNLFTLMLGLP
                     TSSSHALIGGLVGAAVTHGGWSTVKLSGLRSVLEAMVLSPFFGFAIGLSFMVVISWLF
                     FQVTRSVATRLFKRLQLISACFMAFSHGANDAQKAMGIITLALLSAGQIPSAEVPTWV
                     IGACALAMGLGTAIGGWSIVRTLGMGIVKLEPVHGFAAETGAAAVLMVTAHIGLPVST
                     THTITSSVMGVGAIKRLSAVRWGVTRRILYAWLFTLPGAAILSMLIYLIMARFV"
     gene            complement(5374..5994)
                     /locus_tag="VLD60_11715"
     CDS             complement(5374..5994)
                     /locus_tag="VLD60_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSB45674.1"
                     /translation="MMFGLIPREEAFFELFKKAAHNMIEGSRLLKEMMENFHAPVDQA
                     KQIKEVEHVGDGITHDIARRLNQTFITPIDREDIHDLASALDDILDVTEAIADRFVLY
                     KVTKPTEMAVKLADILHQASVAVGSGVDRLGLPHPDIKECSVQVNSLENEADRVSRDA
                     ISALFEKETDPMAVIKWKEIYENFEAGTDRCEDVANILERIALKHH"
     gene            complement(6087..7079)
                     /locus_tag="VLD60_11720"
     CDS             complement(6087..7079)
                     /locus_tag="VLD60_11720"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HSB45675.1"
                     /translation="MIRTRIIGTGSYLPERVVPNREAASALGIDPKRVFRLTGIEERR
                     WAAPMEASSDLALEAARQALQAAGLPATAVEAILLSTTSPDMAFPSTACHLQRKLGCG
                     GVPAFDVSASCSGFLYGLSMADAMIRSGQIRTCLVVAAEVKSRSLDPQDADTALLFGD
                     GAGAVVVQAESETGQPGRGILGVRLHSDGSQHQLIRVAAGGSRTPTSMESVAARDHAL
                     RMQGAPLFRLAIRRLEQAIREILKEFGVELHDVAQMVLHQANGRILSQLTKRLGVSPT
                     VVCSVIERYGNTSSASLPIALDHAARAGYVRPHELLLLGSFGGGLTWATGLVRW"
     gene            7288..8142
                     /gene="bamD"
                     /locus_tag="VLD60_11725"
     CDS             7288..8142
                     /gene="bamD"
                     /locus_tag="VLD60_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HSB45676.1"
                     /translation="MIACSSTSKTVDSEGKKALSGTDESIFLGDTIEKNYDPNVIMKR
                     GEAFFDKEEYAEAIVEYNHFLDLHRNHTLAPYAAFRIGESQMNRAKGIDRDPEPIQKA
                     IDSFERLRRDFPGSRYDGQAAQKIQECHDLVAQMHLFVGQFYYRRESYLAAAHRFEQI
                     MKVYPDKSVAPDALYFLALSYHDLGADDWAAEKLTLLAQKYPNSPHSSEGAKLLAKIG
                     GSKPDILLAKKPEPTPSAADPSTPPTQAPFSVGNGFVPSAPTSSIRIPSAVSLGQGFV
                     SCHLGAWC"
     gene            complement(8342..9088)
                     /locus_tag="VLD60_11730"
     CDS             complement(8342..9088)
                     /locus_tag="VLD60_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSB45677.1"
                     /translation="MSQSITQISLIAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDAGARSKFKATIVANALLFIAGFSVVFIAFGASASLIGQLLITYQDHIRRLGGVLI
                     VVFGLYLLGILNINFLQMEHRFQFRNRPVGYLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTDSLLNGVLLLSSYSLGLGLPLFLTALGVDHFLAYFKQARAYLWGVSTVSGVLL
                     VIVGVMIYANSLTMITSFLERYGIGWYLGQ"
     gene            complement(9101..9652)
                     /locus_tag="VLD60_11735"
     CDS             complement(9101..9652)
                     /locus_tag="VLD60_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB45678.1"
                     /translation="MIRAVLQGGLLLGLTGLAAGFTPSVAAEPGSPVAVAQRGVVKSG
                     DAAPNFQLRDMNGQLVALSDLRGKVVLLNFWATWCGPCRIEMPAMEQLYRTYPRKDFE
                     ILAVSTDAQGVAVTRPFQQENHLTFPILHDADFRVGLAYGARTLPMTFMVDRQGIVRQ
                     QIFGARDWGAPEAHQLIQMLMKS"
     gene            complement(9649..10275)
                     /locus_tag="VLD60_11740"
     CDS             complement(9649..10275)
                     /locus_tag="VLD60_11740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB45679.1"
                     /translation="MSEFLLPTSPSVPRATGGSRFLILVVGVAIFAAVFSIVWMQSDK
                     YEPLEVGKEAPDFALTDLNDKPIRLSDFRGKVVFLNFWATWCKPCREEMPSMEILNKN
                     FEKDGLVILAVSIDRVTTTKDIPPFVKGMNLTFPVLIDSWGKTDKPYKRMGVPETFII
                     DQQGVIREIVIGPRDWTRLDSLQVLTKLLNVTPKAAERTPQNPVGNQG"
     gene            complement(10496..11410)
                     /gene="ccsB"
                     /locus_tag="VLD60_11745"
     CDS             complement(10496..11410)
                     /gene="ccsB"
                     /locus_tag="VLD60_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HSB45680.1"
                     /translation="MARSLFLFDMTFWLYLASLTLYIAYLFAKRPSMMLAPAGHPTEN
                     FDERDGAWATQLGQVATLVTVFGWMVNTAALFTRAFERLQHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLELRYKIKAIGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAEQRGSRSRFYAAFPDLETIDELGYKAIMI
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGRRAA
                     IYAVFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            complement(11492..12925)
                     /locus_tag="VLD60_11750"
     CDS             complement(11492..12925)
                     /locus_tag="VLD60_11750"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HSB45681.1"
                     /translation="MNGQPDTLPQQEAQKARTPGLGWEEFSRELVEFFASIKLAMFLF
                     LFIAITATIGTVIQQGERPETYIQEYGENGYRWFLRLGFTDVYHTWWFTSLLGLLCVN
                     SLTCFYKRFPGVWRSMKQDKVSVSLAFIKGMKQQAEVSVNATKESIAKQLAQYFSEKG
                     YRVLAKNDTGEVTLYATKGVLGRVGAHMAHLSATVIVFGGLLGTYYGFQEFGVCLEGQ
                     TYHIPRGNFDLTVDKFWIDYHENGSVKSYNSTLTVVEGGQAKMTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEVARVNIKDKETDKVVKTIDLEWQKEQTLADLNLKISVTDFVAD
                     FAFNSTEKKVYSKTIEHANPAVKLAVNERSTVQATPWIFYQFPDLFEIKDSKYQFELI
                     GYKPRKYTGLQIAKNPGINIVWLGSTMIVVGITLSSFIYHRRLWTKIIPAGDGVIVHI
                     GGTTHKSQIDFQKEFRKLTEKIRTFSA"
     gene            13287..14435
                     /gene="metK"
                     /locus_tag="VLD60_11755"
     CDS             13287..14435
                     /gene="metK"
                     /locus_tag="VLD60_11755"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSB45682.1"
                     /translation="MRNNYLFTSESVTEGHPDKIADQISDGILDAMLAKDKYSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRQVIKDVGYNDASWGFDSHTCSVLTAIHQQSS
                     DIAMGVDSGGAGDQGLMFGYATNETSELMPMPIVLAHRLTRRLAEVRKKHILPWVRPD
                     GKSQVTVEYRNGKPVRIDTIVVSTQHSPEVTNKQIERGIMEKVIKPVMPKGLYDPASV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMA
                     RYIAKNIVAAGLADKCEVQLAYAIGVADPVSVLVDTKNTERVSVDNLDKLVRKHFPMT
                     PRGIIEHLKLRRPIFKKTAAYGHFGRNEPEFTWEKTDKAKILRKEAGL"
     gene            14556..15815
                     /gene="ahcY"
                     /locus_tag="VLD60_11760"
     CDS             14556..15815
                     /gene="ahcY"
                     /locus_tag="VLD60_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSB45683.1"
                     /translation="MDYDVRDIKLADQGKLKIEWAEATMPVLRLIRKRFKRQQPLKGV
                     RVTACLHVTTETANLAITLKAGGADVRLCASNPLSTQDDVAAALVQHEGIPTFAIKGE
                     DNATYYRHIESAIAHRPHLSMDDGADVVSHLHSKRKDLLKNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLICGSVVVVVGYGWC
                     GRGIAMRAKGMGADVIVTEIDPLKGLEAVMDGFRVMPMEQAASIGDFFVTVTGNLHVI
                     RSEHFAVMKDGAIVCNSGHFNVELDIPSLEKLAKKRRVVRTGVEEFTLKKNGHRVSLL
                     GEGRLVNLATAEGHPSSVMDMSFANQALGAEYIVKNYKLLDKRVYPVPPAIDKEIARL
                     KLAGMGVAIDTLTAEQKKYLASWEMGT"
     gene            complement(16092..16949)
                     /locus_tag="VLD60_11765"
     CDS             complement(16092..16949)
                     /locus_tag="VLD60_11765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSB45684.1"
                     /translation="MMKRPTNCSWVWVGNQIFGWLVVLCMLAGAGCAAGHSGLDRLAS
                     EQSRFVDLTHSFGVDTIVWPTEQDFRLVLQQEGETPGGYYYASNRLDMAEHGGTHIDA
                     PIHFARGGQTLDQVPIERLIGVAVRIDVTAQCARDRDYRVTIKDFERWEVAHGRIPSR
                     AIVLIDTGFARYWPSRQDYLGTELKGQEGVRALHFPGMGHEAAAWLVRVRQVKAVGID
                     TASIDYGQSTGFETHVVLLSQNVPVFENLGDLSGLPDREFEVIALPMKIAGGTGGPLR
                     VIAVLPSAP"
     gene            17113..18315
                     /locus_tag="VLD60_11770"
     CDS             17113..18315
                     /locus_tag="VLD60_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45685.1"
                     /translation="MRSTNFAPLVLSNYEISPEQGFLPQDPPELLPDSPTLNHLGHEL
                     PKLLSARTVRRFIDEQHQLLPSIPPTWGTEDYRAAMRILSFAGHAYVWEVPEQPAAKL
                     PPQLAQPWHDVAQQLGRPPVLSYASYALDNWRRLDPTKPIELDNIVLLQNFLGGQDEE
                     WFVVVHIQIEREAGPGLAGLLRAMKGAAEDKEDEVLIGLTSLASAQTAMRDTLLRMKE
                     RCDPYVYYTRVRPYIHGWKNSPSHPNGLVYEGVESYAGEPQQFRGETGAQSSIVPCLD
                     AGLGIAHAPDPLTVYLQEMRDYMPPRHRLFLCSLEQASDDLGRPLLSGFVRDRKFRRS
                     DLWTAYCTCVDLLAQFREIHIGYADSYIHRQHQAHASNPTAVGTGGTPFMTYLQKHLD
                     ETKQAISS"
     gene            18451..18822
                     /locus_tag="VLD60_11775"
     CDS             18451..18822
                     /locus_tag="VLD60_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45686.1"
                     /translation="MLETIVVAAVLYVCPGDVYSDTRGPGCKPLEQSNKEGFSTIPEA
                     PEFNSISPARAPVDAGGETRDSQTRLPAASAQECAMYEEWVKLSTKSSSIGARDLSPG
                     EYERWTNLKQVFGNTPPPNCR"
     gene            19013..19138
                     /locus_tag="VLD60_11780"
     CDS             19013..19138
                     /locus_tag="VLD60_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45687.1"
                     /translation="MSVMCSLAACKAQEGMCGHEKVMLSVALLAAIGFGAYFLIG"
     gene            complement(19374..20873)
                     /locus_tag="VLD60_11785"
     CDS             complement(19374..20873)
                     /locus_tag="VLD60_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45688.1"
                     /translation="MMAAPGNQPPPFYDPQELLNAQPVIVSVVDPATYTVQFQNATGL
                     KKFGDISGQTCHEKIAGCPAPCAFCKMPDAVSTGTMTMNEVALPNNQWLLVQWSKAVT
                     ADGRTHVIEAITDITERKRLEDSAHKAEKMEALSRLAGGVAHDLNNLLTVIMGANEQV
                     SRHAADSQQAQVPIKRIQDAVERAADLTHHLVAFSHHQVVEPTVLDVNSILSEMEPKI
                     HQLAGDTVTLTVALAVECEPVVMARGQIEDMIRTLVANAREAMPQGGQLLISTSLKSV
                     TEVEAQAHGVKPGVYVVMAVRDTGHGIEPVTQAHLFEPFFSRGQVQTGRGLGLASLYG
                     IVRQSGGFVEVSSKQGAGALFEIYLPRCDEGQTESHAVHRPETLSGSETILLVEDDED
                     VRVAVGDMLRRTGYEVQEAADGIEALYQLGQSSVHPQLVLTDVMMPRMTGPQLAKRLD
                     AMMPSVKILFMSGYTDGTLEPVDGQRPAFILKPFNSKDLLRKVRETLAS"
     gene            21274..22236
                     /locus_tag="VLD60_11790"
     CDS             21274..22236
                     /locus_tag="VLD60_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HSB45689.1"
                     /translation="MVSVSPHDPTLARTLILDELFDLSLYKALRDVTQPDVHHTLDEL
                     IKVETSHLAFWQKFFNLHLDELNIGRRFKLSVMMFVCKLFGSTAVHLVLEAIEVHGVR
                     KYLALWGEYQGKPLGEALRGILMDEFKHEDVLVTELTKRKINPERIRNIFLGLNDGLV
                     EILGAVSGFFGAFGSATMVLIAASTTAVAGALSMAAGAFLALSSEKEVKTTELARKVF
                     LGEESEVTPMEESPLGSAVVVGVAYITGALVPVLPVLVGAKDALFSVFTAGLMVILVS
                     TVLSFLSGMDVKRRIVLNLVIITVAVTVSYSIGLVAKQIWGIHI"
     gene            complement(22680..24314)
                     /locus_tag="VLD60_11795"
     CDS             complement(22680..24314)
                     /locus_tag="VLD60_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB45690.1"
                     /translation="MRVFLIHVRDPQFYALPAKTRAKNGNIRVMGFPPIGIMSLSSVL
                     KQAGHECVMFDQANPDTPNDVIIEEIKRCKPDLVGLSFLSTTSYPYAKMLARKIRLAD
                     STVKLAFGGVFASLNAGLVKLQCSEVDFVCRGDGEQLLLDLLAHLNDPEGVAGLTWMK
                     DGLVINNPGRPMERQLDQWPLPDRESLKLDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCIFCDIPIFNEGKWRARSALHVVNELKYLEAQGYGAVYFVDDHFLLQPKRIEAICN
                     GVIDAGLRIQWGIEGRVDSVAQHLFPVMAKAHCRTVMFGIESGSQKILDRLKKEQTLE
                     EVTMAVKNAKKAGIEIVHGFFTVGNPGETVADMQATFEFAAKLPLDTFGFNRLCVYRG
                     TPLWQEYVKRGLVSDVTDWYKYFKCSEIDPTCLPGEVINTVRQEGLKKLFLYKLTHYP
                     LQTYRLLRRFMRFMPVRDVIYLIVKPFLGQKKGATKAEVLSRAIEHAELKDAAAQLTQ
                     LSDEMLHKVLESSREERMRIQQEAESSRELPMMPAR"
     gene            complement(24566..25438)
                     /gene="sucD"
                     /locus_tag="VLD60_11800"
     CDS             complement(24566..25438)
                     /gene="sucD"
                     /locus_tag="VLD60_11800"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSB45691.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKIVAGVTPGKA
                     GQDVEGIPVFNTVRDAVKKEQCDTSLIFVPPPFCADAILEAADAGIKLVICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKKGIVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFERDPETQAIVMIGEIGGDAEEKA
                     AEFIKKNVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTAAEKMKTLEAAGVKVVKN
                     PAEIGNAVKKALGR"
     gene            complement(25598..26776)
                     /gene="sucC"
                     /locus_tag="VLD60_11805"
     CDS             complement(25598..26776)
                     /gene="sucC"
                     /locus_tag="VLD60_11805"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSB45692.1"
                     /translation="MNVHEFQAKSLFAQFGVPVPRGKEITAPDAATAWANELNTPVFV
                     VKAQIHAGGRGKAGGVKITKDKGAVAGFAKELLGKTLVTHQTGPKGRTVHRLLMEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAAHTPEKIIKEAIDPAVGFQGHNG
                     RNVAFALGLQNIEPAVINPFVKLLDNLYRLFMEKNAALVEINPLIITKEKTLVALDGK
                     VSFDDNGLFKHEDVQKMRDLNEEEPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNAEEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            complement(26789..>27671)
                     /locus_tag="VLD60_11810"
     CDS             complement(26789..>27671)
                     /locus_tag="VLD60_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSB45693.1"
                     /translation="IELLSDDNGACTGAILKDLDNQRHVVVAAKAVILATGGIGRLHI
                     QGFPTSNHYGATGDGLCLSYRLGARLAHIDTFQYHPSGAVYPEQLIGALVTEGIRSEG
                     GQLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWLDTPLLDAEHGPG
                     TLEKHFPAMVMQFDRFGIDISKDPVLIYPTLHYQNGGVKIDTNSETNVKNLFVAGEAS
                     GGLHGRNRLMGNSLLDLMVYGKRSGLTAAGRTGSMTQGKLTLKHLQRFRAEAKKHGNP
                     SGVISPMILPAYTRKVS"
BASE COUNT         6157 a         8213 c         7505 g         5796 t
ORIGIN      
        1 caacacgctg cgaacgtctt tcaaccggca gagggtctta ttgatgaggt actcgctatc
       61 gccgttcctg tagaggcggc gcgtgatcat gagatcctga tactccgaca actggctggg
      121 aagaccggac tgactgtcga ccttcaattg gtctaacccg ctgatcacca gcgacacctc
      181 ggccatgcct agcggcttgc gtacttccgt cccgttaaag atcacgtctt ccatcttttc
      241 actgcgcaga gtcttggtgc tttgctcgcc caggacccag agaatggcat ccacgacgtt
      301 gctcttgccg ctcccgttcg gcccaacaat ggctgtcacc ccattgggaa actcgatgcg
      361 agcttcggcg aacgacttaa accccaacat ctcaagcgac ttgaggtaca tgaatgtctc
      421 cttctgtagg gatcttattg gggagcatca gtgggaatgt cacgggtcgg ttttgtacca
      481 caggctgcac atggaaatca aagaaaatac cgcctgtagt gggagcaggt aggacggcct
      541 acaatatttg gggaatcaga agagtgacgc cgggacggga ggttatcccg ccgaggcggc
      601 tacggattta cctgaagact cgatggtgat gagctctttc ggaagaagaa actcgacatc
      661 ctcctcgatg acggtcagat cttcgacctc ggctgtaccg taagtcttga ggtaggccac
      721 aacctcctcc acgaggacct ctggtgccga agccccggcg gtgatcccga tcgccttcac
      781 attcttgagc caggcaggat tgatgtcggc ggctgagtcg atcaagtacg agtcaatccc
      841 gcagtgttct cccaactcgc gcagacggtt cgaattggag ctattgggcg agccgatgac
      901 gagaatgacg tcgacgagcc tggacaattc cttgactgca ttctggcggt tttgcgtcgc
      961 atagcaaatg tcttcctggt gcggcccttt aatgtgggga aatcgcttgt gcagcgcacc
     1021 cacgatgtcc cggcattcat cgacactcaa cgtggtctgc gtgacgtatg acagattggt
     1081 tctgttttcg acctggagca tctcgacgtc ttccactgac gacaccaggt gaaacttgtc
     1141 cggaatctgc ccgagggttc caatgacttc cggatggccg gcgtggccga tgagaatgag
     1201 gtcatatccc tgggtatagt cacggttgac ttcattgtga actttgatga cgagtgggca
     1261 ggtcgcgtcg atgacgtgta agcggcggcg gttcgcctcc tcccataccg atttcgccac
     1321 cccgtgcgcg ctgaaaatta cgacggagcc ctcgggaact tcactcagtt cttccacgaa
     1381 caccgcgccc ttttggcgca acgagttgac gacgtgacgg ctatggacga tttcatggcg
     1441 gacataaatc ggcgccccgt atttcttgag cgataagtcg acaatatcga tcgcccgatc
     1501 aactccggcg caaaatcctc gtggattagc aagatagatc ttcatcccgg ccacctctcc
     1561 ctcttcacgg acactgcgat cacattacgt gactcccacc agccgcgtca acagaattac
     1621 ccccttgttg gaagcacagg ttcacccctg gccatcttga cagccgaacc cctcccccgt
     1681 aggctggagg caccccccat ggccagtgcc cctgccaaaa aaatcctgat cgtcgaagat
     1741 gagcgcgaca ttctccattt ggtcaagctg tatctggaga aggaagggtt ccgcacgatt
     1801 acggcggcga caggcgcaga aggactgaac agtgccaaat gcgacaaacc cgatctcatc
     1861 gtcctggacc tgatgctgcc ggaaattgat gggctcgaag tctgtaaacg ccttcgatcg
     1921 gctccggaga ctgcgatgtt gccgatcatc atgctgacgg ccaaggccga agaatccgac
     1981 actgtcatcg gtctggaact cggcgccgat gactatgtca cgaaaccctt tagccccaaa
     2041 gcgctcgtcg ctcgcgtcaa agcgctgttt cgccgcctcg accgcagcca gcaggataac
     2101 ctcacggtgt ttcgctacgg tcccctgacc atggacctgt cccgtcatga agtgacggtc
     2161 cgcggcaaag atgtctcgct cacagccaaa gaattcggtc ttctggaaca cctgttgcgt
     2221 aaccccggcc gagtcttgac cagagaagtg ctcttaaatc atgtctgggg ttacgactac
     2281 ttcggcacaa caagaaccgt cgatgtccac gtccgacggc ttaagcagaa gatttctttg
     2341 ctcgacgacg ccatcctttc cgtgaaatca ctcggctata aactgaagga ccctacgggt
     2401 aacgcgtgac tcttgacccc ttgagaaaga tgcattccga tctcataccg gtcatctgcg
     2461 ttgacttctc acccttcacc ttttgcccat caccataacc tatgaggctt tcaattcgct
     2521 ggaaagtgac actcgccacg ctgtttgcct tagtgtgcgg gttcgcgatc gcgggattcc
     2581 ttgcaacccg ctcattggaa gaacaggagc tggcccagtc gaaccagaca cttgaaatca
     2641 gaaccaactt gatcgcgttt gaattgcgtc ctctcctccc caccctacct gcgactcccc
     2701 atttgcaaat catggttcgc gagctgagcc agcgtgctct cgcgcgcgtg acgttgattg
     2761 atgacaccgg acgcgtcctt gccgacagcg cagtccagga tcgagacctg ggcaccgtcg
     2821 agaaccaccg cacgcggccg gagatcgagc aagccgtcgc gaccggattc ggcacggatc
     2881 tgcgcgccag tcatacgacg ggtgagcgca ccttgtaccg cgccatccgc ctgagcgggc
     2941 cggccgaaac ttcctcgccg ttctatcttc gactagggct ccccctaacg acacttgatc
     3001 tggagcttgc caaactcaga cgcaatctga ccctcgcctt tgtcagcgcg tttctgatcg
     3061 cggtcggctt gagcatttgg cttgccggaa gtttgaccaa acccctgtta gatatggcgg
     3121 ctgtcgctcg ccaactcgct gcaggcaacc acacggtcca catccggacg tccgcccgcg
     3181 acgaagtcgg gctcctggcc gataccctga atcacatgac cgatgagttg aaaaccagaa
     3241 tcgaggaact ctccgaagac cgctcccagt tgctggccat gctgacctca atggttgaag
     3301 gcgtgatggt gctggactat cgcggccgcg tgctccaagt caatccggcc ctggagcgga
     3361 tgttcggcgt gaccagggcc gaaacgcgcg ggcgcctgtt ctccgaggtg ttcggacatc
     3421 ctgaattgaa ttcgctggtc tcgaccgtgc tggccacgag ggccggccag gaagacgaaa
     3481 tcatccttgc tccgagcggg cgatgccttc acatcgaagc ctccgtggcc ggaggcgagc
     3541 aggagaacga ggcctgtgcc gtgctcgtct tccacgacat caccgaactc cggagactgg
     3601 aaaagatcag gaaggatttc gtggccaacg tgtcgcacga actcagaacc ccgctgactt
     3661 cgatcaaggg atacgtcgaa gcgctgctgg acggcggtaa ggacgatccg gacacgagcg
     3721 tgcgttttct ggacatcatt ttaaagcaga gcgatcggct gaatttgatc ctggaagatc
     3781 tgctgcagct ttccaagatc gagtccggcc aggtccaatt caagcgggag cccctgcaca
     3841 tcggaacggt cgtcgaacgc acgatcgcca tgatcaagcc tctcgcggac aagaagcagc
     3901 atcagctgat cgctcaggtc tcctccgatc tgccactcgt gaccggtgat caagaacgac
     3961 tggtgcaagt cctggccaat ctggtggaca atgcgatcaa atacaccccg gaagggggac
     4021 agatcacggt cgcagcgcgc cggattcctg ccgccaagag cggaacggct gcccagcaag
     4081 acgggatcga gttgagcgtc gccgacacgg gcatcggcat tcccgaacag gatcgacccc
     4141 gagtcttcga acgcttctat cgggtggaca aggctcgctc ccgcgaactg ggaggaacag
     4201 gccttgggtt ggcgatcgtg aaacacatcg tggaaggaca tggagggcaa gtttgggtgg
     4261 aaagaaacct gccgaccggc agccggttcg tggttcgatt gccggcctga tccggcggcc
     4321 tgccacttag acgaatctgg ccatgatcag atagatcagc atggagagaa tggcagcgcc
     4381 cggaagggtg aagagccagg catagaggat tcttctcgtc actccccagc gtaccgcaga
     4441 gagacgtttg atcgcgccga cgcccatgac cgaggacgta atggtgtgcg tcgtgctgac
     4501 gggcaatccg atatgggcgg tcaccatcag cactgctgcc gcaccggtct cggcggcaaa
     4561 tccatgaacc ggttcgagct tgacgatgcc catgccgagc gtccgaacga tcgaccagcc
     4621 tccgatcgcc gtccctaatc ccatcgccag cgcgcaggct ccgataaccc aggtggggac
     4681 ttcagctgaa ggaatctgcc cggcggacag caacgccagc gtgatgattc ccatcgcttt
     4741 ttgcgcatcg tttgccccgt ggctgaacgc catgaagcac gcggaaatga gctgcagtcg
     4801 tttgaagaga cgggttgcga cggagcgtgt gacctgaaag aacagccaac tgatcaccac
     4861 catgaaggag agcccgatcg caaatccaaa aaacggcgaa agcaccatgg cctcaaggac
     4921 ggaacgcaga ccggacagct tcaccgtact ccacccccca tgggtcaccg cagcccccac
     4981 aagcccaccg atcagcgcat gagacgaact ggtcggcaat cccaacatga gcgtgaacag
     5041 gttccaacag attgctccgg ctagcgccgc cgccaccatc tgctgggtga cagaggttgg
     5101 atcgacaata ccagacccga ccatcttcgc aactgccgtc gacatgaacg cgccggccac
     5161 attcagcagc cccgccccca tcacggcgat aagggggctc accacacgcg tcgaaaccac
     5221 tgtagcgatg gcgttcgcac tgtcgtgcca gccgtttgaa aaatcgaata acagggccaa
     5281 caccaccaca accagtaaca ttccggtcag ttcaggcatc ttagactcgt gagaagtaag
     5341 gagtaaggag tgaatggaaa cgcatcggct gactcagtga tgtttgagcg cgatgcgttc
     5401 aagaatattg gccacgtctt cgcaccgatc ggtccccgcc tcgaagttct cgtaaatctc
     5461 tttccacttg atgacggcca tcggatcggt ttctttctca aatagcgcgg agatcgcatc
     5521 gcgcgagaca cgatccgcct cgttctccaa actattcact tgcacgctgc actccttaat
     5581 gtcgggatgg ggcagcccta gacggtccac cccgctcccg accgccaccg aagcttgatg
     5641 cagaatatcc gccagtttca cggccatctc agtcggcttc gtcactttat acagaacaaa
     5701 tcgatcggcg atcgcttccg tcacgtcgag aatatcgtcc agtgcgctgg ccagatcgtg
     5761 aatgtcttcc cgatcgatcg gcgtaatgaa ggtctggttc aatcggcggg caatgtcatg
     5821 ggtgataccg tcaccgacgt gctcgacttc cttgatttgt ttggcttgat cgaccggcgc
     5881 atggaaattc tccatcatct ccttcaagag acggctgcct tcgatcatat tatgcgcggc
     5941 cttcttgaag agctcaaaga acgcttcttc tctgggaatc aagccaaaca tcatcgcgtt
     6001 gctcctctgc cggtgaaatg cctactcact ctctcgcccg gtttattgaa acgtgaagaa
     6061 cgcgtgacag gttgtcacga acgacttcac catcggacca gtccggtagc ccaggttaac
     6121 cctccgccaa agctgcccag caggaggagc tcgtgtggcc gaacatagcc ggcccgcgcc
     6181 gcgtggtcca gtgcgatcgg gagcgaagcg gaggaggtat tgccataccg ttcaatcacc
     6241 gagcagacca ccgtcggcga aacacccaag cgcttcgtga gctgggagag aatacgccca
     6301 ttggcctggt gcagcaccat ctgtgccacg tcatgcaact ccacaccaaa ctctttgaga
     6361 atctcgcgaa tggcctgttc gagccgtctg atcgccagtc gaaagagcgg cgccccctgc
     6421 atccgaagcg catggtccct cgctgccacc gactccatgc tcgttggcgt acgcgatcct
     6481 cctgctgcaa ctcgaatcaa ctggtgctgg gaaccatcgg agtgaagacg aacacccagt
     6541 atcccgcggc caggctgccc ggtctcgctt tccgcctgca ccacgaccgc accggcacca
     6601 tccccgaaca aaagcgccgt atcggcgtct tggggatcca aggaacgaga cttgacctcc
     6661 gctgccacga ctaagcaggt tcttatctgc ccactgcgaa tcatggcatc ggccatggaa
     6721 agaccgtaga gaaagcccga gcaggaggcg gatacgtcga aagccggcac gccgccacag
     6781 cccaattttc gctgcagatg acaggctgtg gacggaaacg ccatgtcagg agaggtcgtt
     6841 gaaagaagaa tcgcttcgac tgcggtggca ggcaacccgg ctgcctgcaa cgcctgccga
     6901 gccgcctcaa gagccagatc ggatgaggcc tccatgggag ccgcccagcg tcgttcctca
     6961 ataccggtta ggcggaagac ccgcttcgga tcaataccca atgcagaagc cgcctcccga
     7021 tttggtacca cccgctccgg gagataagaa cctgttccga taattctcgt ccgtatcatc
     7081 gctgaaatgc caaggggtta cattgcgccg gcgggattat aggtctcgga aggacggggg
     7141 tcaacccaac tagcagaaac gttgcattta tcccggtctc ctgctagaag atatcattat
     7201 tccatgagaa accgaccgat atccctgact acctggccac caagcaggag tctttctctt
     7261 gccctgggaa cggctatgct ctgcgcgatg atagcttgtt caagcacctc gaaaaccgtc
     7321 gactcagagg gcaagaaagc attaagcggg accgacgaat cgatcttcct gggggatacg
     7381 atcgaaaaga attacgaccc caacgtgatc atgaaacggg gcgaagcctt ctttgacaaa
     7441 gaggaatacg ctgaagcgat cgtcgagtac aaccacttcc tcgatctgca tcgcaaccat
     7501 accctcgccc cctatgccgc gtttcggatc ggggaaagtc agatgaatcg agcgaaaggg
     7561 atcgatcgcg accccgagcc aatccagaaa gcgatcgatt catttgaacg actgcgaagg
     7621 gatttcccgg gaagccgata tgacggtcag gcggcacaga aaatccagga gtgtcacgac
     7681 cttgtcgcac aaatgcatct gttcgtcgga cagttctact atcgaagaga atcgtatctt
     7741 gccgcagcac accgcttcga gcagatcatg aaagtgtatc cggacaagtc ggttgcgcca
     7801 gatgccctat acttcctggc cctcagctac catgatctcg gtgccgacga ttgggccgct
     7861 gaaaagctga ccctgcttgc acaaaagtac ccgaacagtc ctcattccag tgaaggggcc
     7921 aaactcctcg ccaagatcgg cggatcgaaa cccgacattc tcttggctaa gaaaccggag
     7981 cccacaccct ccgctgccga tccctcaacg cctcccacac aagcaccgtt tagcgtcgga
     8041 aatggttttg tcccctcggc cccaaccagt tcgatccgga ttccctctgc agtctctttg
     8101 ggacaaggat ttgtgagctg ccatctcgga gcctggtgct gattttcgac actgaagcca
     8161 tcagccttca gtaaaacctc aaccttcttt atgtgacttg tgcgcgtcga cggcgagcag
     8221 cccaatgcct acgacttgtt ccaggatcgg tggacaggat tccatggcga aacgcttgcg
     8281 ggattcggca gagaggtttg agtctgtggc tgaaggctga ccgctggttg ctgacggctc
     8341 ttcactggcc gaggtaccag ccgatgccgt atcgctcgag aaagcttgtg atcatcgtca
     8401 gagaattcgc gtagatcatc acgccaacga taaccagcaa gaccccactg accgtcgaca
     8461 caccccacaa atacgcgcgc gcctgcttga agtaggctaa gaaatgatcg acgcccagcg
     8521 ccgtcagaaa aagcggtaac cccagtccta acgaatagct gctcagcaac agcacgccgt
     8581 ttaacaacga gtcggtcgtg ctcgcataga gcagaatggt cccgagcacc ggcccgacgc
     8641 acggagtcca gccggccgca aacgcaatgc cgatgaggaa agaacccaga taaccgactg
     8701 gtctgttccg gaattgaaac cggtgttcca tctggaggaa gttgatattc agaatcccca
     8761 agagatagag gccgaacacc acaatcagca cgcccccgag acgacggatg tggtcctgat
     8821 aggtaatcag cagctggccg attaagctcg ccgaggcgcc gaatgcgatg aacaccaccg
     8881 agaatccggc gataaacaac agggcgttcg ccacaatggt cgctttgaat ttcgaccgcg
     8941 cacccgcatc ggtcaactgt tcgatcgaca ggcccgtgat gtaggaaata taagacggga
     9001 caagcgggag cacgcaggga gagacaaacg acagtaaccc ggccgagaac gccgcgatca
     9061 atgagatctg tgtgattgat tgcgacatgg gttcgtcaga ctacgacttc atcagcatct
     9121 ggatgagctg gtgcgcttcg ggagcccccc agtcgcgagc cccgaaaatt tgttgccgca
     9181 caatcccctg gcgatcgacc atgaacgtca taggcagcgt gcgggcacca taggctaagc
     9241 cgacgcggaa gtccgcgtcg tggaggattg ggaaggtcag atggttttcc tgctgaaacg
     9301 gcctggtcac cgccacccct tgcgcatcgg tcgagacagc caggatttca aagtcttttc
     9361 gcggataggt ccggtagagc tgttccatgg ccggcatttc gattcggcag gggccacacc
     9421 aggtcgccca gaaattcagc aagacgacct tcccgcgcaa gtccgacaac gccaccagct
     9481 gtccattcat atcgcgcagc tgaaagttcg gggcggcatc accggatttg acgacgcctc
     9541 gttgcgcaac agcaaccggc gagccgggct ctgccgcaac ggatggtgtg aaacccgcgg
     9601 ccagtcccgt taatcccaac aacaatcccc cctgcagaac cgctcggatc atccttggtt
     9661 ccccaccggg ttttgcggcg ttcgctccgc cgccttcggc gtgacgttga gcagtttggt
     9721 caacacttgc aaactatcca gtctggtcca atctctcggc ccgatcacga tttcgcgaat
     9781 cacgccctgc tgatcgatga taaaggtttc cggcacgccc atgcgcttgt acggcttatc
     9841 ggttttcccc caggagtcga tcaacacggg aaaggtcagg ttcatccctt tgacgaacgg
     9901 cggaatatcc ttcgtcgtcg tcactcgatc aatgctgacg gccagaatca ctaacccgtc
     9961 cttctcgaaa ttcttgttca agatttccat ggagggcatt tcttcccgac agggcttaca
    10021 ccaggtcgcc caaaaattca ggaagacgac tttgccgcga aagtccgaca gcctgatggg
    10081 tttgtcgttg aggtcggtga gcgcaaaatc cggggcttct ttgcccacct cgagcggctc
    10141 gtatttgtcg ctttgcatcc agacgatgct gaagacagcc gcgaagatgg ccacccccac
    10201 gacaaggatc agaaaacgcg acccgccggt ggctcttggg acagacggtg acgttgggag
    10261 taagaactca gacaagtttc ccccttgcag ctaattagag gatgctcaaa atggtcttcc
    10321 aacaaggccg cagggagcga ggaccccgag gacgtccata ccacgcttcg cttgagccgc
    10381 tcgattccat caaatgcgag cggagagtta cctggcctcc aatagacccg tatcgtacgt
    10441 tgaggggaac gagcatccga gaacgaagtt ggtagccatg ttcagcatcc tacggctatg
    10501 cgtaggcatg aaggcccgac aacagaaaat tcacacccca gaagcagaat atcaccatca
    10561 gaaatccgaa caccgcatag atggccgccc gccggccttc ccagccgcga gtcatacgag
    10621 cgtgaatgta cgcgccatag atcaaccaga cgatcaacga ccaggtttcc ttggggtccc
    10681 agctccagta accgccccac gcgtaattgg cccacatggc gccgagaatg atgccgaacg
    10741 cgaggagagg gaaaccgatc atgatggctt tgtagcccaa ctcatcgatg gtttccaggt
    10801 cggggaacgc cgcatagaag cgtgatcggc tcccccgttg ctccgcccgc tctttgaaga
    10861 ggtacatgag gccgagcccg cccgccatcg cgaatgcggc atagctggtc agggtgacgg
    10921 acacgtgaat gtagatccag tagctgttga gcgcaggaac gagcggctcg gcagtctgat
    10981 agcgataagg cagcagggat gccgccccca tggcgataaa accgatgccg accacgaaag
    11041 ctccgatggc tttaatctta tagcgtaatt ccaggaggac atacccgaga atgatagccc
    11101 acgacacata ggccatcgct tcaaactggt tcgaccaggg cgcgaacgta cccgaatgct
    11161 gcaatcgctc gaacgcacgc gtgaacaacg ccgccgtgtt caccatccag ccaaatacgg
    11221 tgacgagtgt cgccacttgt cccaactgcg tggcccaggc tccgtctcgc tcatcgaaat
    11281 tttcagtcgg gtgtcctgcc ggagcgagca tcatcgaagg tcgctttgca aacagatagg
    11341 cgatgtataa cgtcagcgag gcgagataga gccaaaacgt catgtcaaag agaaagagtg
    11401 aacgagccat cgtgtcctcc gtctaccaca tcagaccggc cactgcccta ctatcatacc
    11461 caaaacagat ccgatttgcc caccctaagc gctacgcgga gaaggtccgg atcttctctg
    11521 taagcttccg gaattctttt tgaaaatcaa tctgactctt atgggtcgtc ccgccgatat
    11581 gcacgatgac accgtctccg gctggaataa ttttcgtcca caaccggcga tggtagatga
    11641 acgatgagag ggtaatcccc acgacaatca tggttgagcc gagccatacg atattgatgc
    11701 cggggttctt ggcaatttgg agcccggtat acttcctcgg cttatagccg atcagctcaa
    11761 actgatactt cgaatccttg atctcgaaca agtcgggaaa ctggtaaaaa atccaaggag
    11821 tcgcttggac cgtgctacgt tcattcacgg ccagcttgac cgccggattg gcgtgttcga
    11881 tcgtttttga atacaccttt ttttcggtgg aattaaaggc aaagtccgcc acgaaatcgg
    11941 taaccgagat tttcaaattc aaatcggcga gcgtctgctc cttctgccat tccaaatcga
    12001 tcgtcttgac gaccttgtcc gtttccttgt ccttgatgtt cacccgggcg acttcaatct
    12061 gatcccaggc gtcgccgtag ctggattgat agaaccagat gcccttgtag acgagaggat
    12121 cattgacggt aatcgtcttc gtcattttcg cctgcccgcc ttccaccacc gttaacgtgc
    12181 tattgtagga tttgacggat ccgttctcgt ggtagtcgat ccagaacttg tccaccgtca
    12241 aatcgaaatt gccgcgcgga atgtgatacg tctgcccttc caggcaaacc ccgaattcct
    12301 gaaacccata ataggttcca aggagtcctc cgaacacaat caccgttgcg ctcagatggg
    12361 ccatatgtgc gccgacacgg cccagaaccc ccttggtcgc atagagcgtc acttcgccgg
    12421 tatcgttctt ggccaagacg cgatatccct tttcgctgaa gtattgtgcc agctgttttg
    12481 caatggattc tttcgtcgcg ttgaccgaca cttcggcctg ctgtttcatc cccttgataa
    12541 aggccagcga gacgctgacc ttgtcctgct tcatcgatcg ccagacgccg gggaaccgct
    12601 tatagaagca ggtcaatgag ttgacgcaga gaagaccgag cagactggtg aaccaccagg
    12661 tgtgatagac gtccgtaaat cccaaccgca gaaaccagcg gtaaccgttc tcgccgtatt
    12721 cctgaatgta tgtttcgggc cgttctccct gttgaatgac cgtcccgatc gtggctgtaa
    12781 tggcaatgaa caaaaacaga aacatcgcca gcttgatcga ggcgaaaaac tcgacaagct
    12841 cacgagagaa ttcttcccac cccagacccg gggtcctggc cttctgggcc tcctgctggg
    12901 gtagggtatc gggctgaccg ttcatcgcgt cccttggtcc tattgcatta acagacacac
    12961 aatccttacg gcccaggaag cacggcagat tgggcaagga cgatcgtgtg ctggaaggcg
    13021 gtcccttctg gacagtggtc gtaccctgtt gcagaacaga gagggaattc cttggaaccg
    13081 tggcatctta caagtgccgg aaaagagctg tcaagcaacc gctccgggca aagaatgtct
    13141 ccccctacta ttgaagtgat ggacaaatca ggccattttc gctatcatgc cccccgattc
    13201 tgcaagctaa cacctccttc tcacacaggg ccatttcccc ctactgagat cgagcgactt
    13261 tcgttcactg aacttttgag gagccaatga gaaataatta cctctttacg tctgaatccg
    13321 tcacagaagg ccatcccgac aaaatcgccg accagatttc cgacggcatc ctagatgcca
    13381 tgctcgccaa ggacaagtac tcccgtgtgg cctgtgaaac catcctcaca accggaatcg
    13441 cctttgtcgc aggggaaatc tcgacgaaag cctatgtcga gattcccgac atcatccgcc
    13501 aggtgattaa ggatgtcggc tacaacgatg cgtcatgggg cttcgattcc cacacctgct
    13561 ctgttctcac cgccatccac cagcagtcga gcgatatcgc gatgggagta gattccggcg
    13621 gagccggcga ccagggattg atgttcggct acgcaaccaa cgaaacctct gaactcatgc
    13681 cgatgcccat tgtgctcgct cacaggctca cacgccggct cgccgaagta cgaaagaaac
    13741 acattctccc ctgggtccgc cctgacggca aatcgcaagt caccgtggaa tatcgcaacg
    13801 gtaagccggt gcggatcgac actattgtcg tctccacgca gcatagcccg gaggtcacca
    13861 acaagcagat tgaacgcggg atcatggaaa aagtgatcaa gccggtcatg ccgaagggtc
    13921 tctatgatcc ggccagcgtg aagcaccaca tcaatccgac cggtcggttc gtcgtcggcg
    13981 gtccgatggg tgacacgggt ctgacgggac ggaagatcat cgtcgacacc tacggcggcc
    14041 acggcagcca cggcggcggt gccttctccg gcaaggatcc cacaaaggtc gaccgttcag
    14101 cctcctatat ggcccggtac atcgccaaga acatcgtcgc ggccgggttg gcggacaagt
    14161 gcgaagtgca actggcctac gctatcggcg tagcagaccc cgtttccgtg ctggtcgata
    14221 cgaaaaatac cgagagagta tccgtcgaca atctcgataa actggtgcgc aagcactttc
    14281 cgatgacgcc ccgcggcatc atcgaacatt tgaagctccg acggccgatt ttcaagaaga
    14341 ccgcagccta cggccacttt ggtcgaaacg aaccggagtt cacctgggaa aagaccgaca
    14401 aggcgaagat cctgcgcaaa gaggcagggc tgtaatagaa ggcgacggcc aaggttacgt
    14461 tcacgcaaac tgaggctcag gcttagggat tgaggctggg attttcactc agcctcgacc
    14521 ttagcctgag ccttcttttc gacgaggagg aatccgtgga ttacgacgtg agagatatca
    14581 agctggctga ccagggcaaa ctcaagatcg aatgggccga agccaccatg ccggtgctgc
    14641 gactgattcg gaaacgattc aagcggcagc agccactcaa gggtgtaagg gtcaccgcct
    14701 gtttgcacgt caccactgaa acggccaatc tggcgatcac attaaaggcc ggcggcgccg
    14761 acgtccggct ctgcgcatcc aacccgctca gcacacagga tgatgtggct gctgcgttgg
    14821 tccagcacga aggcattccg accttcgcga tcaagggcga ggacaacgcc acgtactacc
    14881 gccacatcga gtccgccatc gcgcatcggc cgcatctgtc gatggatgac ggggccgatg
    14941 tcgtctcgca cctgcactcc aaacgcaaag atcttttgaa gaacgtcatc ggcggcacgg
    15001 aagaaaccac caccggcgtc atccgtctgc gcagcatggc ggaaaagaaa gtgctcaagt
    15061 tcccggtgat ctccgtgaac gacgccgaca ccaagcatat gttcgataat cgctacggca
    15121 caggtcaatc cacaatggac gggatcgtgc gtgcgaccaa ccgcctcatc tgcggctccg
    15181 tcgtggtcgt cgtgggctac ggctggtgcg ggcgcgggat cgcaatgcgc gccaagggga
    15241 tgggcgcgga tgtcatcgta accgagatag acccgttgaa gggcctggaa gccgtcatgg
    15301 acgggttccg cgtgatgccg atggaacagg ctgcgtcgat cggcgacttc ttcgtaactg
    15361 tcaccggcaa cttgcacgtg atccgcagcg aacatttcgc cgtcatgaag gacggcgcga
    15421 ttgtctgcaa cagcggtcac ttcaacgtcg agttggatat tcccagcctc gagaagctgg
    15481 ccaagaagcg gcgggtcgtc cgcactggcg tcgaggagtt taccctcaag aaaaacggcc
    15541 atcgcgtgag cctgttgggc gaaggccggt tggttaatct agccacggca gagggacatc
    15601 cctccagcgt catggacatg agcttcgcga accaggcgct cggtgcggaa tacattgtga
    15661 agaactataa gctgctcgac aaaagggtct acccagtccc accggcgatc gacaaggaaa
    15721 tcgcgcggct caagcttgcg ggcatgggcg tggccatcga tacgctgacg gcggagcaga
    15781 agaagtatct cgcatcctgg gaaatgggaa cctgatcgca ggtccagtcg gcgaacggcg
    15841 ctgaaatgct catgtcactc attccccaat gcatcctttc agttgcgcgc gtcagagctg
    15901 ttcgctacca ccagctcact cacctataat ccgcctgctc attgagagta aaagaaggag
    15961 gccgcctcat ctgaggcggc cttcggtttt tggagcaacc gcggtatgaa ttgctgaaat
    16021 ctctatctgg aaccaagaag tagttagaca agtaaccgtg gcatgctgaa cttcctgcta
    16081 cgtggagacg tctatggtgc tgatggcagc accgcgatga cgcgcagcgg ccctccggtc
    16141 ccaccggcga ttttcatcgg cagggcgatc acctcaaatt cacgatccgg cagcccgctg
    16201 agatctccaa gattttcaaa gaccgggacg ttctgggaga gcagcacgac atgcgtctca
    16261 aatccggtcg attgaccgta atcgatcgag gcggtgtcga ttccgacggc tttgacctgc
    16321 cgcactcgca caagccaggc cgccgcttca tggcccatgc cagggaaatg gagcgctcgt
    16381 acaccctcct gccctttcaa ttctgttccc agatagtctt gtcgcgacgg ccagtatcgg
    16441 gcaaagcccg tgtcgatgag cacgatcgcc ctgctgggaa ttcgcccatg cgccacctcc
    16501 catcgttcga aatccttgat ggtgacgcgg taatcccgat cacgggcgca ttgggctgtg
    16561 acatcaattc tgacagcaac accaatcaaa cgttcgatcg gaacctgatc cagcgtctgc
    16621 ccacctcggg caaagtgaat gggcgcatcg atgtgagtcc cgccatgttc ggccatgtcc
    16681 aggcgatttg aggcgtagta gtacccgccc ggcgtctccc cctcctgctg gaggacgagc
    16741 cggaagtcct gttcagtcgg ccatacaatc gtgtccaccc cgaaggaatg agtaagatcg
    16801 acaaatcgcg actgctcact tgcaagacgg tcaagtccag aatgaccggc agcgcatccc
    16861 gcgccagcca gcatgcatag cacgacaagc cagccgaata tctgattccc cacccacacc
    16921 catgagcaat tcgtcggcct cttcatcatg gcatgcccct ttgtggtgaa aacctctggc
    16981 aaatattgtc agtccgatgg gcaagaaagc aaacaggcca atctgttttt gcaatcgctg
    17041 aatcgattga gattcaagct agctgtggta gagtgaaccc aggcgccgag gcggtgacta
    17101 ccggaggctc cgatgagatc gacgaacttc gctcccctcg tcctttccaa ttacgagatc
    17161 tcgcctgaac agggttttct tccgcaagat ccgcctgaac ttcttcccga ctctccgact
    17221 cttaaccatc tggggcacga gctgcctaaa ttgctgagcg ctcggacggt ccggcggttt
    17281 atcgacgaac aacaccagct gctaccatcc atccctccaa catggggcac agaggactat
    17341 cgagccgcaa tgcggattct ttcattcgcg gggcatgcct atgtgtggga agtaccagag
    17401 caaccggctg cgaagttgcc gccgcaactg gcgcaaccat ggcacgacgt ggcccaacaa
    17461 ctgggccggc cccccgtgct ctcctatgca tcctacgccc tcgataactg gcgcaggctc
    17521 gatccgacta aaccgatcga actcgacaat attgtgctgc tgcagaattt tctcggcggg
    17581 caggacgaag aatggtttgt cgttgtgcat atccagattg agcgggaagc gggaccaggg
    17641 ctggcgggcc ttctgcgggc gatgaagggt gcagctgagg ataaggagga cgaggtcctg
    17701 attggactca catccctggc gtcggcccag acggcgatgc gcgacacgtt gctccgcatg
    17761 aaagaacgtt gcgatcccta cgtctactac acccgcgtgc ggccttacat ccatggatgg
    17821 aagaacagtc cgtcacatcc gaacggactc gtctacgagg gcgtcgagtc ctatgccggc
    17881 gaaccccagc agttccgcgg cgaaaccggc gcgcaaagct ccattgtgcc atgcctcgac
    17941 gccgggctcg gcatcgccca tgcgccggac cccctcaccg tctatctcca agaaatgcgg
    18001 gactatatgc ccccgcgcca tcgtttgttt ctctgctcgt tggagcaagc cagcgatgat
    18061 ctcggtcggc cgttgctctc cggctttgtc cgcgaccgca aattccgtcg ttccgatctc
    18121 tggacggcct attgtacctg cgtcgatttg ctcgcacagt ttcgtgagat tcacatcgga
    18181 tatgccgaca gttacattca ccgccaacat caggcccacg cgagcaatcc tacggccgta
    18241 ggaacgggcg gcacaccctt catgacctac ctgcaaaaac atctggacga gacgaagcag
    18301 gcgatcagct cctagagaca agtattgggc gttgggcaga gcatgcctgg caccgccata
    18361 ctcaggtcct gcccaacact gtgatttact ttctggggtg ggaaacccgt gctagggtgg
    18421 tcgcatagtt ctgaataatg gagggctccc atgctggaaa ccattgtcgt tgcagcggtc
    18481 ctgtacgtct gcccgggtga tgtctactcc gatacgcgtg gccccggttg caaaccgctt
    18541 gagcagtcca acaaggaagg cttcagcacg atcccggagg cgccggagtt caattcaata
    18601 tcgccggcgc gcgctccggt cgatgccgga ggcgaaacga gggactcgca aacccgcctt
    18661 cctgcagcat cagcccagga atgtgcgatg tatgaggaat gggtcaaatt atccaccaag
    18721 tcgagcagta tcggagcccg cgatctctca ccgggagaat atgaacggtg gacgaacttg
    18781 aagcaggtgt tcgggaacac ccctcctccc aattgccggt aaccagcact cgttcttcct
    18841 ggcggctcat ccgtttcttg accacatcgg gctgagccgc cgctcgactt catcatcgcg
    18901 atctctcccc tctctggtcc gttcgaccaa caatcccagc gtcgagaaca ccgttgattc
    18961 ctctgtacag gcggcgtatc gtcttaacag atcttgagac gggaggactc ccatgtcggt
    19021 catgtgctcc ctggcagcct gcaaggctca ggaagggatg tgcgggcatg agaaggtgat
    19081 gctgagtgtc gccctgctcg cagctattgg cttcggggcc tacttcctga tcggctgacc
    19141 agcggtatcg gaaagcgcta cgctccatgc agtctatcaa acgtgagggg taggtgtgcc
    19201 ctctccctga agtctcaacg atcatcgccg ctataacgcc gcacaattcc ccaccggact
    19261 taatatcgaa cctggaagag cggtgcggca tgtcgcctga cgacccgctg tagaagactg
    19321 atgaggatga atcgcttgca gaaggagaac cgcgacacgg cgaatagagg catctagctc
    19381 gcgagggttt cacgaacctt tcggagcaga tctttggagt taaacggctt cagaatgaag
    19441 gcggggcgct gtccatccac cggctcaaga gtcccatccg tatagccgga catgaacaaa
    19501 attttgaccg aaggcatcat cgcgtcgaga cgcttggcaa gttgagggcc cgtcatgcga
    19561 ggcatcatga catcggtgag aaccagctgt ggatgcaccg acgattgtcc caactggtag
    19621 agcgcctcga ttccatcagc cgcttcttga acctcatacc cggttctccg caacatgtcc
    19681 ccgacggcca ctctcacatc ttcgtcatcc tcgaccagca agattgtctc ggagccgctc
    19741 aatgtctccg gccgatggac ggcatgcgat tcggtctgac cctcatcaca ccgtggcaga
    19801 tagatttcaa acaatgcacc ggcaccttgc ttgctcgaca cctcaacaaa ccccccgctt
    19861 tgccgaacga tgccatagag gctggcaagc cccaatcctc tcccggtttg gacttgaccc
    19921 ctggaaaaga acggctcgaa aagatgcgcc tgcgtcaccg gttcgatccc atgccctgta
    19981 tcacgaaccg ccatcacgac gtagacacct ggtttgaccc cgtgcgcctg cgcctcgacc
    20041 tctgtgacgc tcttcagtga ggtcgagatc agcagctgtc cgccttgcgg catggcttca
    20101 cgcgcgttgg caaccaaggt tcggatcatg tcctcgatct gtccgcgagc catgaccacc
    20161 ggctcacatt cgactgctaa ggcgacggtc agcgtgacgg tatcgccggc taactggtgg
    20221 atcttcggtt ccatctcgga caaaatcgag ttcacgtcaa gtacagtcgg ttccaccact
    20281 tgatgatgac tgaatgcgac tagatgatgc gtcaaatctg ctgcccgctc cacggcatcc
    20341 tgaatccgtt tgataggaac ctgggcctgc tgactatcgg ccgcatggcg ggatacctgt
    20401 tcgttggccc ccatgatcac ggtcagcaga ttgttgagat cgtgtgccac tccgccggcc
    20461 agccggctta acgcctccat cttttccgcc ttatgtgccg agtcttcaag gcgcttgcgc
    20521 tcggtaatat cggtgatcgc ttcgatgacg tgcgtccgcc catcagctgt cacggctttg
    20581 gaccattgga caaggagcca ttgattgttc ggaagagcca cctcgttcat tgtcatggtt
    20641 cccgtgctca ccgcatcggg catcttgcaa aacgcgcaag gggcggggca tccggcaatt
    20701 ttctcgtgac aggtctggcc ggagatatcg ccgaacttct tgagccccgt tgcgttttgg
    20761 aattgcaccg tataggtcgc aggatcaacg acagagacga tgactggttg agcgtttaac
    20821 aattcctgag gatcgtagaa gggaggaggc tggttaccag gcgctgccat catggtctcc
    20881 aaaccaaatg gggttctgaa atcctgggcg caacaacacc ctgactctca gagccgcatt
    20941 gcatcgaaca gcatagacca taccagggca tagggattca agagaatcag gttctctctt
    21001 gcaataatga ggagttacgc gtcgctccag tctgttacac gatgttgaat tcggtgggca
    21061 tctgaatcca agtagatcag atacagtatc ggatttgata cgcctgacta cacaaacagg
    21121 cgggaggatc tgttccttcc cgtgaacccg ggatccacgc acttaccagg cgctgattct
    21181 ccttcctctt gctccatcgg gtactttgct ataatcccat ccctgtttcc agctcttgtt
    21241 gtctcattcc cttgcccttg cgaggttggt cacgtggttt ctgtctcccc acacgatccg
    21301 acgctggcgc ggacgctgat ccttgacgag ctgttcgatc tgtctctcta caaggcactc
    21361 cgcgatgtga cacaacctga tgtgcaccat accctcgatg aattgattaa ggtggaaact
    21421 tctcacttgg cattctggca gaagttcttc aaccttcacc tcgatgagct gaatatcgga
    21481 cgacggttca aactttccgt catgatgttc gtatgcaagc tgttcggatc gacagcggtg
    21541 catttagtcc tcgaagcaat cgaagtccat ggcgtgcgca agtatttggc gctgtgggga
    21601 gagtatcagg gcaagcctct cggcgaagcc ttgcgcggga ttttgatgga cgagttcaag
    21661 cacgaggacg tgctcgtgac ggaattgacg aagcggaaga tcaacccgga aagaattcgg
    21721 aacatttttc tgggacttaa cgatggcttg gtggaaattc tcggcgcagt gagcggattt
    21781 ttcggcgcat tcggcagcgc cacgatggtc ttgatcgctg cctcgacgac cgccgtggca
    21841 ggggccctct ccatggccgc cggcgcgttc ttagcgttga gcagcgaaaa ggaagtgaaa
    21901 acgacggaac tggccaggaa agtgttcttg ggagaagaat cggaggtcac gccgatggaa
    21961 gaatcgccgc tgggatcggc cgtggtggtg ggagtggcct atatcaccgg cgcgctggtg
    22021 ccggtgttac cggttcttgt cggtgcaaag gatgcgctct tctcggtctt cactgccggc
    22081 ctcatggtga ttctggtgtc gaccgtcttg tcgtttttgt cgggcatgga tgtaaaacgc
    22141 cgcatcgtgc tcaatttggt gattattact gtggcggtga ctgtcagcta ttcgataggc
    22201 cttgtcgcga aacagatctg gggcattcac atttaggcat caatcctggc tcaccccaca
    22261 acctcgtgat cttccggaac tggactcact tccgacggag tcaccgaacc gacaccacga
    22321 ccaacactac gttcggctgg agccctcagc gctgactttt gtgaaatgtg ggaggaacgg
    22381 ctgaatctgc ttcagaaaca taggggcgct ccgccgcatc cagttcataa agagtgccca
    22441 tccccaaacg gccacggcaa tcagaagcgg cgtgaggggg agtcaacact ctgcaatcca
    22501 cagttaacac caggaaatga aaaagatatc ggcttgaagc agcagggcaa gatacccaaa
    22561 cacccctgcg ctccagggga gcacattgat ccactataca ccgaacgaga caaagaccgt
    22621 aaagacgaag actccctcga taaatagcgc tttgtggtaa ggcaagaccg accggggtgt
    22681 tagcgggcag gcatcattgg cagttcgcgc gagctttccg cttcttgttg aatcctcatc
    22741 cgctcctcgc gtgaggattc caacaccttg tgcagcatct cgtcgctcag ctgggtcagt
    22801 tgagcagccg catctttcaa ttcggcatgc tcgatggcgc gagacagcac ctcggccttc
    22861 gtggcgccct tcttttgacc aaggaatggc ttgacgatga ggtagatgac atcgcgcacc
    22921 ggcatgaaac gcatgaacct ccgcagtaag cggtaggtct gtagcgggta atgcgtgagc
    22981 ttgtacagga agagcttctt caatccctcc tggcgaactg tattgatgac ttcgccgggc
    23041 aagcaggtgg gatcgatttc cgaacacttg aagtatttgt accaatcggt cacgtcgctg
    23101 accaacccgc gcttcacata ttcctgccag agcggtgtgc cgcgatagac acagaggcga
    23161 ttgaacccga acgtgtccag tggcagtttc gccgcgaact cgaacgtcgc ttgcatgtct
    23221 gcgacggttt cgccgggatt gcctaccgta aagaagccgt ggacgatttc aatccccgcc
    23281 ttcttggcgt tcttgacggc catggtcact tcctccagcg tctgctcttt cttgagccga
    23341 tccagaatct tctgactgcc gctttcgatt ccgaacatga ccgtccggca atgcgccttc
    23401 gccatgacag gaaatagatg ctgcgccacg gaatccaccc gaccttcgat tccccattga
    23461 atccggagcc cagcgtcgat cacgccgttg caaatggcct caatgcgctt cggctggagc
    23521 aagaagtgat cgtcgacgaa gtagactgcg ccatatccct gcgcttcaag atacttcagc
    23581 tcattcacca catgcagcgc gctgcgcgcg cgccacttac cttcgttgaa gatcggaatg
    23641 tcgcagaaaa tgcagggcca gggacaacca cgtgacgttt gcatggtggt aaaccgctcc
    23701 atcgacaaca ccgccggcac atcgagcggc atcgactcga caaagtcgag tttgaggctt
    23761 tcgcggtccg ggaggggcca ttggtccagc tgccgctcca tcggacggcc cggattgttg
    23821 atgaccaggc catccttcat ccaggtcaaa ccggccacgc cttctggatc gttcagatga
    23881 gcgaggagat cgagaaggag ctgttcgccg tctccgcggc agacaaaatc gacctccgaa
    23941 cattgcagct tcacaagacc ggcattcagg ctggcaaaca cgccgccaaa cgcgagcttg
    24001 accgtggaat cggccaaacg aattttccgc gcaagcatct tcgcgtaggg atagctcgtc
    24061 gtgctgagaa aactcaatcc cacgaggtcc ggcttgcagc gtttgatctc ttcgatgata
    24121 acgtcgttcg gtgtatcggg attcgcctgg tcgaacatga cacactcgtg gccggcctgt
    24181 ttgaggacgg acgagagcga catgatgccg atgggcggaa agcccatcac gcgaatattg
    24241 ccgtttttgg cgcgagtttt ggctggaagt gcgtagaatt gcggatcacg aacgtgaatg
    24301 aggaagactc gcaacgatac tcctgggtta ggcctcgacc tgaacaggga aaccaaactg
    24361 ccgagggtct agttgggaaa cgaatcgcaa cgggaaggga cgtatcacct ctaagttaca
    24421 ataccacgtt atcgcgagtg gggggaagcg aataagtgga tcagcggccg cacaggttca
    24481 ttgtagacgg ttgccagagg atttgggcta ggggaaagac tcgaacggtt tgcagctgca
    24541 acagcagtgc tggggcacac cgtccttagc ggcccagcgc cttctttacc gcgttgccga
    24601 tctcggccgg attcttgacg accttcacgc cggccgcctc gagcgttttc atcttctctg
    24661 cagcagtgcc cttgccgccc gaaataatcg ccccggcgtg gcccatgcgg cgtcctggag
    24721 gcgcggtaat cccggcaata aagctgatga ccggcttctt gacattcttc ttgatgaact
    24781 cagcggcctt ttcttctgca tcaccgccga tttctccgat catcacaatg gcctgtgtct
    24841 caggatctct ctcgaacaag ggcagcacgt cgacaaatcc tgtcccattg accggatcgc
    24901 cgccgatgcc gacgcaggtg gtttcgccca atcccaatgt cgagagctga tgcaccgctt
    24961 catacgtgag ggtgccgctg cgggacacga ccccaacgat ccccttctta tggatgaagc
    25021 caggcatgat gccgattttc gcctcgtcga ccgtaatgac accggggcaa ttcgggccga
    25081 tcaatcgcac gtcccgaccg tggagcgcgc gcttcacctt caccatgtca ttaaccggaa
    25141 tcccttcggt aatacagatc acgagtttga tgccggcgtc ggccgcttcc aggatcgcat
    25201 cggcacagaa aggcggcggc acaaaaatca acgaggtgtc gcactgctct ttcttgaccg
    25261 catcgcgaac ggtattgaag accggaatcc cttcgacatc ctgcccggcc ttgcctggcg
    25321 tcacgccggc cacgatcttc gtcccgtagg ccttacactg ggtcgcatgg aacgagcctt
    25381 ccttgcccgt aattccctgc accaccaccc gcgtattctt attgacgaga atgctcacga
    25441 ttccctcttt tctttcttat ttcaccttcc catgaccggg aagcaatctt tcggaggcgc
    25501 agggctaagc ccccactgcg ccgcgtccaa cgaggccctt ctgagggcgc gcgttgcgcg
    25561 agcacagggg cttatccctg tgcctccatt tgtcgtctca cgccgctttt ccggtcagct
    25621 tcacaatctt ttgcgccgct tcccacaaat cgttggccac ttccagtttg aggccggatt
    25681 cggccagcag cttgcggcct tcttcggcat tcgtgccctg cagacgcacg accaacggca
    25741 ccgtgatctt cacttccttg gccgcctcga ttacgccatg cgcaatacgt tcacaacgga
    25801 caatgccgcc aaagatattg atgaaaatgc ctttcacgtt cgggtctttc agcagaatcc
    25861 ggaagccggc cgccacggtc tctttcgtgg cgccgccgcc gacgtccagg aagttggccg
    25921 gctcgctgcc cgccagcttg atgacatcca ttgtcgccat cgcgaggcct gccccgttga
    25981 ccatgcagcc gatgttgcca tctagcttca catagttcag gttgttcgcc gttgcttcga
    26041 tctccagcgg ctcttcttcg tttaggtctc gcatcttctg gacgtcttcg tgcttgaaga
    26101 gcccgttgtc atcaaacgaa actttgccgt ccagcgccac cagcgtcttt tccttcgtga
    26161 taatgagggg gttgatctca accaacgccg cgttcttttc cataaataag cgatagagat
    26221 tgtccagcag cttcacgaag ggattgatca cggcaggctc gatattctgc aatcccagcg
    26281 caaaggcgac gttgcgaccg ttatggcctt ggaatccgac ggctggatcg atcgcttcct
    26341 tgatgatctt ttccggcgtg tgggctgcga cttcttcgat ttccatcccg ccttcggtgc
    26401 tggcgatgaa ggtcggccag ccggtgtcgc gatcgacgag gatggagaga tacagctcct
    26461 tggcaatatt cgcaccttct tccatgagga gacggtgcac ggtgcggcct ttgggaccag
    26521 tctgatgggt gacgagggtc ttcccaagca actccttcgc gaaaccagcc accgcaccct
    26581 tgtcctttgt gatcttgacg cctcccgcct tgccgcgccc gccggcatga atctgcgctt
    26641 tcacgacgaa gactggcgtg ttcagttcat tggcccaagc ggttgccgcg tccggagcag
    26701 tgatctcctt cccgcgtggc accggtacgc cgaattgggc aaacagagac ttagcttgga
    26761 actcgtgcac attcatgctg tacccctctc agctgacttt tcttgtgtag gccggcagga
    26821 tcatcggcga gatgacaccg ctgggattgc catgcttctt ggcttcggcg cggaagcgtt
    26881 gcagatgttt cagcgtgagt ttcccttgtg tcatggaccc ggttcgtccg gcggcggtta
    26941 atcctgaccg cttgccgtaa accatcagat cgagcagcga attgcccatc aaacggttac
    27001 ggccgtgcag cccgccagaa gcttcccctg caacgaacag attcttcacg ttggtttccg
    27061 aattcgtatc gatcttcacg ccgccgtttt ggtagtgcag cgtcggatag atcaggacag
    27121 gatctttgct gatatcgatg ccgaaccggt caaactgcat caccatcgcc gggaaatgct
    27181 tctcgagcgt gccgggcccg tgttctgcat ctagcagcgg cgtatccagc cagacaccga
    27241 cgcggccgga catagtcctg atcccgcggc cctcttcaca ctcgcggatg atggacgagg
    27301 agacgacgtc gcgggtgtcg agctcattga cgaaccgctc gcccttcgca ttgacaagtt
    27361 gtccgccttc cgaacgaata ccttccgtca ccagcgcgcc gattaattgc tcagggtaca
    27421 ccgcgcctga agggtgatac tggaacgtgt cgatgtgcgc cagtctggcg cccaagcgat
    27481 aggacaggca cagaccgtcc cccgtggcac catagtgatt gctcgtgggg aatccttgaa
    27541 tatgtaagcg gccgatgccg cctgtcgcga gaatcaccgc cttggctgcg accaccacat
    27601 ggcgctggtt gtcgagatcc ttgagaatcg ccccggtgca ggccccgttg tcgtcgctga
    27661 gtaattcaat c
//
LOCUS       DATIFR010000203         6217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2056962, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000203 DATIFR010000000
VERSION     DATIFR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056962"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VLD60_11815"
     CDS             <1..437
                     /locus_tag="VLD60_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45694.1"
                     /translation="TPQACSAWHTFDQSPANANTLRNIIDGMTGGTYTSPGTSPGDTL
                     NFINGNVASAFPNLINLYNAKKDANGNWDVFVPVYQSPDCTPPNGPIPIIGYAEARIT
                     NVQGAPNFLIEATILCNIFEGNTVGGGPPYGPVLSTIPGLVE"
     gene            465..1688
                     /locus_tag="VLD60_11820"
     CDS             465..1688
                     /locus_tag="VLD60_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HSB45695.1"
                     /translation="MARLIAVSIDCRNEDARQTFEEIVSRRRDYLITRGQGTGAADML
                     LLELDELRPQQTFGHIRALLSTSPDLEVFLTASRTDPQVLLEAFRLGVKEFLPQPLTR
                     QEVEPALARFEERFNGRVSGVETQSGRVVSVIGARGGVGASTVATNLAISVQQASPGD
                     PVSLMDLDMHGGDLGLFLDLHPSQGLKHLSNDISRLDETIVRSSLARHSSGLHLLPSG
                     YEGFEGTEQVPGSTMRVIGLLRSMHRHVFVDCGHVLEPAVKEALDCSDQVIVVTTLSL
                     PAIRRTKRLLEALGSAQYQAGKLLVVVNRYINDQKDLLSETENMLGLRMAGLIPNDYG
                     TASDALDHGKPLTIMASRTTIGQWYLRGTDQLIGDKVAVDGATAGKEPSKTASFLGRY
                     LPSFGLEMKGKPSVV"
     gene            1750..3063
                     /locus_tag="VLD60_11825"
     CDS             1750..3063
                     /locus_tag="VLD60_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSB45696.1"
                     /translation="MLTMKQTNGTELASHYQELKDRLHQRVIELLDLNAISAMSQEAV
                     TAQLTKLIEQLLQQESVPLNQRERAQITQDILHEVLGLGPLEPLLADTTVNDILVNGH
                     KQVYVERAGRLEHTSVRFKDDAHLKKIIEKIVSRVGRRIDESVPMVDARLADGSRVNA
                     IIPPLAIDGPSLSIRKFSKDPLQMYHLIEKRSLTPEIGELLKAIVQARLNVLISGGTG
                     TGKTTMLNILSGFIPNNERILTIEDAAELQLRQDHVVRLETRPANIEGKGEVAQRELV
                     KNALRMRPDRIILGEVRGAECLDMLQAMNTGHDGSLTTVHANSCRDALTRIETLVSMA
                     GLNLATKAMRHYVSSALDVIIQIARLSDGTRKLVSVQEVVGMEGDLITLQELFTFQQT
                     GLDEKRNVKGRFKATGVRPKFAERLIAKGVALPAEIFDPQKVYEC"
     gene            3057..4022
                     /locus_tag="VLD60_11830"
     CDS             3057..4022
                     /locus_tag="VLD60_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB45697.1"
                     /translation="MLTALIALGIFGTILVFLQGCALAHRMMAPRGTDRAVERIQKWS
                     APEAATQIEIVRKESLSDIPWLNDILMKMRRSQPLRVLHRQADCRVPLGIFVLATPLL
                     ALSGFLFAVSTHLPMVVALVIAASAGVLPAGYLYWLKSQRMAMFERQLPEALELVSRA
                     LKAGHAFSVGLKLVGDEAADPIGVEFRRVFDEVSMGVSLPEALQNMTDRLDCVDLRFF
                     VTSVLVQRETGGNLAEIIDSLAGLIRRRFELQLKVRALSAEGRFSAIILFGLPFVVGG
                     ALYIMNPEYMSTLFTDPIGKNMVMIGSFLMVTGAIIMKRMVNIKV"
     gene            4033..4989
                     /locus_tag="VLD60_11835"
     CDS             4033..4989
                     /locus_tag="VLD60_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB45698.1"
                     /translation="MDVPLIVALVTFLAVALASSAVFFYFNSREALQTWRRRADGASA
                     AVDSEATPAGMAEQLMAQLRYLLEWFARMNQPSNVEEVRATRRLLINAGYRSGKAPVF
                     FVGAKLLLAIVVVCLLAIIPAKVLGFPSAAKLTFFYVLAAACGYYAPAYWLRRAIAVR
                     KDALQRAIPDALDLMVVCVEAGLGLDQAIGRVGEEVKRAHPELSDELNMLTLELRTGV
                     ARQEALRNLAHRTDLEEVRNLVALLVQTDRFGTSIGQALRVHADSMRTTRRLKAEELA
                     AKLPVKLLIPLIFFIFPSMFMVVIGPACIKMIRVLFPAMTGH"
     gene            5224..5934
                     /locus_tag="VLD60_11840"
     CDS             5224..5934
                     /locus_tag="VLD60_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45699.1"
                     /translation="MTKDHPASYWPTPLAVLAWQDSPASQVAQSRAVRFLLDTSGIHF
                     PRKPDAPWAHNTILKGWPWINDTHSWVEPTATCVIALRAAGHGQHDRVHEAIRMILDR
                     QLPHGGWNYGNTLMFGKELHPMPESTGAALTGLAGMVDQNTVSRSLDYLQGEIPRLRT
                     PISLGWSLLGLAAWELWPSNGAALVERCLANQLRYGDYDTSALCLLFLGALAGESHTA
                     TPLFLHGGRNQAPAVIGQ"
     gene            5943..>6217
                     /locus_tag="VLD60_11845"
     CDS             5943..>6217
                     /locus_tag="VLD60_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45700.1"
                     /translation="MLDHVDSTSTGGLTRRDVLLAASAGLVIGTGLLLVPKRYFRRPQ
                     RAQAFVAKAANYQLDIADLISRGILELGIAPEELKGKRILLKPNLVET"
BASE COUNT         1294 a         1604 c         1883 g         1436 t
ORIGIN      
        1 gaacgccgca ggcctgttcc gcctggcata cattcgatca gtcaccggcc aacgcgaata
       61 cgttgagaaa tattatcgat gggatgactg gcggcacgta tacatcaccc ggcacatcgc
      121 caggggacac cctgaatttt atcaatggca acgtggccag cgccttcccc aacctcatta
      181 atctctacaa tgctaagaaa gatgccaacg gtaactggga cgttttcgtg cctgtgtatc
      241 agtctccgga ctgtacgcca cccaacggcc cgattccgat catcgggtat gccgaagccc
      301 ggatcacgaa tgtccaaggc gcaccgaatt ttttgatcga ggcgaccatc ctgtgcaaca
      361 ttttcgaggg gaatacagtt gggggtggtc ccccgtatgg gccggtcctc tcaacgattc
      421 caggtcttgt cgaataattc gaataaccga ggagaatagc tggcatggct cgattgattg
      481 cagtgtcgat cgattgtcga aacgaagatg ctcggcagac gtttgaggaa atcgtctcgc
      541 gtcggcgcga ctacctgatc accagggggc aggggaccgg tgcggcggac atgttgttgc
      601 tcgaattgga cgaattgcga ccgcaacaga catttggcca catccgtgca ctgctgagca
      661 catcgccgga tctcgaggtg tttctcacgg cttcgcggac ggacccccag gtgctgctgg
      721 aagcgttccg gctcggggtg aaggaattcc tacctcagcc cctgacgcgc caagaagtgg
      781 agccggctct tgcgcggttc gaagaacgat tcaacgggcg tgtgtccggc gtggagacac
      841 agtcggggag ggtcgtcagc gtgatcggcg cgaggggcgg ggtgggcgcg agcacggtcg
      901 ccacgaatct cgcgatttcg gtccagcagg cgagcccggg ggatccggtg tcgttaatgg
      961 acctcgatat gcatggggga gatctcggat tgttcttgga cctccatccg tctcaaggac
     1021 tgaaacatct gtcgaatgac atctcgcggc tggatgaaac gattgtccgg agcagcttgg
     1081 cgcgacattc ctcggggctc catctactcc cgtcagggta tgaagggttt gaagggactg
     1141 agcaagtccc cggcagcacg atgcgcgtga tcggtcttct ccgatccatg catcggcatg
     1201 tctttgtcga ttgcgggcat gtactcgagc cggcggtcaa ggaagccctg gattgttccg
     1261 atcaggtcat cgtcgtcaca acgctgtcat taccagccat ccgtcgtacc aagcggttgc
     1321 tggaggcatt gggatctgca caataccagg cgggcaagtt gctggtggtc gtcaatcgct
     1381 atatcaatga ccagaaggac ttgttgagcg agacagaaaa tatgttgggc ctgcgcatgg
     1441 ccggactgat cccgaacgac tatgggacgg ccagcgatgc tctcgaccat gggaagccgc
     1501 tgacgatcat ggcatcaagg acgacgatcg gacaatggta cttgcgaggg acggatcagc
     1561 tcattggcga caaggtggct gtagacgggg caacggctgg gaaagaaccg agtaaaacag
     1621 cgtcgttcct ggggcggtat cttccgagct tcgggcttga gatgaaaggg aagccatcgg
     1681 tcgtttaagc ggttcagatg atgcggcatg gatcagtaac tggggcgaaa aggagggcaa
     1741 gtaggaacta tgctcacgat gaaacagaca aacgggactg agctcgcgtc ccactaccag
     1801 gaactgaagg accgtctcca ccagcgcgta atcgagttgc ttgatctcaa tgcgatcagt
     1861 gcgatgtcgc aggaggctgt gactgctcag ttgaccaaac tgattgagca gctcttgcag
     1921 caggaatctg tccccctcaa tcaacgggaa cgggctcaga ttacgcaaga cattctccac
     1981 gaagtgctcg ggttggggcc gttggagccg ttactggcgg atacgaccgt caacgacatc
     2041 ctcgtgaacg ggcacaagca ggtctatgtc gagcgagccg gacgtcttga gcatacttct
     2101 gtccgattca aagacgatgc gcacctgaag aaaatcatcg agaagatcgt ctcgcgtgtc
     2161 gggcggcgca tcgacgaatc ggtgccgatg gtcgatgccc ggcttgcgga cggctcccgc
     2221 gtcaatgcga tcatccctcc gctcgcaatc gatggacctt cactctcgat cagaaagttc
     2281 agtaaggatc cgctacaaat gtatcatttg atcgagaaac ggtcgttgac gccggagatc
     2341 ggtgagttgc tgaaggcgat cgtgcaggcc aggttgaacg tgttgatttc cggtggaacc
     2401 ggtactggaa agacgacgat gttgaacatt ctgtcaggat tcatcccgaa caatgagcgg
     2461 atcttgacga tcgaagacgc tgccgagtta cagcttcgcc aggatcatgt ggttcgtttg
     2521 gaaacccgcc ctgccaatat tgaagggaaa ggcgaggtcg cacagcgtga gttagtaaag
     2581 aatgccctcc gtatgcgtcc agaccggatc atcctgggtg aggtgcgtgg ggcggaatgt
     2641 ctcgacatgc ttcaggcgat gaacaccggt cacgacgggt cgctcacgac tgtccatgcc
     2701 aattcctgcc gggatgctct gaccaggatt gagaccttgg tgtcgatggc cgggctcaat
     2761 ctggcgacga aggcgatgcg ccattatgtt tcgtccgcgc tggatgtcat tattcagatc
     2821 gccaggttgt cggatgggac tagaaagctt gtcagtgtgc aagaggtcgt cggcatggag
     2881 ggcgacttga tcaccttgca ggagctgttt acattccagc agaccggtct tgatgagaag
     2941 cggaacgtca aaggtcgatt caaggcgacc ggcgtgcgcc cgaagttcgc cgaacggctg
     3001 atcgctaaag gggtcgcact tccagccgag atttttgatc cgcaaaaggt gtacgaatgc
     3061 tgaccgcact gattgcgctg ggtatcttcg ggacgatcct cgtgtttctc caagggtgtg
     3121 cgctggcaca tcgcatgatg gctccccgcg ggacggatcg agcggtcgaa cggatccaaa
     3181 agtggtcggc tcctgaggcc gctacgcaga tcgagatcgt tcgaaaggag tcgctcagcg
     3241 acattccttg gttgaacgat atcttgatga agatgcgccg atcccaaccc cttcgcgtac
     3301 tccaccgaca ggcggactgt cgggtcccgt tggggatctt tgtcttagcc acaccgctct
     3361 tggcgttgag cggattcctg ttcgccgtat cgacgcatct tcctatggtc gtggcgcttg
     3421 ttatagcggc cagtgcaggt gtccttcctg ccggctatct ctattggctc aagagtcaac
     3481 ggatggcgat gttcgagcgt cagttgccgg aagcgcttga gttggtctcg cgggctctga
     3541 aggccggaca tgccttctcg gtcggattga agctggttgg ggatgaagcc gccgatccca
     3601 tcggtgttga gttccggcgt gtgttcgatg aagtctccat gggcgtgtct cttccggagg
     3661 cgctccaaaa tatgactgat aggctggatt gcgtggattt gcggttcttt gtgacctcgg
     3721 ttctcgtcca gcgggaaacc ggaggtaatt tggctgagat catcgattca ctggcaggac
     3781 tgatccggag gcggttcgaa ttgcaactaa aagtcagagc cttatcggcc gaagggcgtt
     3841 tctccgcgat tattttattc ggtctccctt ttgttgtcgg tggcgctctc tacataatga
     3901 atcctgagta tatgtcgacg ctgttcacgg atcccatcgg gaagaatatg gtcatgatcg
     3961 ggagttttct catggtgaca ggcgcgatca tcatgaagcg catggttaat atcaaagtct
     4021 aaggagtcaa gtatggatgt ccctctgatc gttgcgctgg tcacctttct cgcggttgcg
     4081 ttagcgagtt cggctgtctt cttctacttc aattccagag aagccttgca gacttggcgt
     4141 cgccgggctg acggcgctag tgctgccgtg gattctgaag cgactcctgc aggaatggcc
     4201 gagcagctca tggcgcagct gcggtacttg ctcgaatggt tcgcccgtat gaatcagccg
     4261 tctaacgttg aggaagttcg tgcgactcgc cgcctcctca tcaacgccgg ttatcgatcc
     4321 gggaaagccc cggttttttt cgtcggcgcc aaactcctgc tcgccattgt cgtagtctgc
     4381 ctgttggcga tcattccggc aaaggtcctg ggatttccca gcgctgcaaa attgacattt
     4441 ttctatgtcc ttgccgcagc ttgtggctat tatgctccag cgtactggtt gcgcagggcc
     4501 attgctgttc gaaaggatgc gcttcagcgg gccattccgg atgccttgga tctgatggtg
     4561 gtctgtgtcg aagcagggtt ggggttagac caggctatcg gtcgagtcgg cgaagaggtc
     4621 aaacgggctc acccagaatt gagcgatgaa ttgaacatgc tgacgttaga actacgaacg
     4681 ggtgttgcac ggcaggaggc gctgagaaat cttgctcatc gaaccgactt ggaagaggtg
     4741 agaaacctgg tcgcactcct tgtgcagacg gaccggttcg ggacgagtat cggtcaagcc
     4801 cttcgggtgc atgccgattc gatgcgcacc actcgtcgac tgaaagcgga agagttggct
     4861 gcaaagttgc cggtcaagct gctgataccg ctcattttct ttatcttccc gagcatgttc
     4921 atggtcgtga ttggtccggc atgcatcaaa atgatccggg tgctattccc ggcgatgacg
     4981 ggccattaag aagaatgggg atcttatttg cctcgctcat cattccggaa gattgtagag
     5041 acatcccttt accgccgctt gctgcctgat ggaggctttg ccggaaggct cggaggtaca
     5101 ttccaagttg actcgactgc ttggggagta cttgcctttc gtgcatgcgg cggatcgaat
     5161 gagatactcg aaaaggtctg cgggatcctg gcccgtgagc aaagcgaaga tggtcgactg
     5221 tgtgtgacaa aggaccatcc tgcttcctac tggcccaccc ctctggcggt tcttgcctgg
     5281 caggattcac cggcaagcca agttgctcaa agtcgtgctg ttcgcttcct gcttgatacc
     5341 agcggcattc actttcctag gaagcctgat gccccgtggg cacacaatac gatcctgaag
     5401 gggtggccct ggatcaatga cacccattcc tgggttgaac ccacggctac gtgcgtgatc
     5461 gcgttgcggg cggcagggca tggtcagcac gaccgcgtcc atgaagccat cagaatgatt
     5521 cttgatcgtc agttgcccca tgggggatgg aattatggga acacccttat gtttgggaag
     5581 gaactccatc ccatgccgga gagcacaggt gccgcattaa ccggtttggc aggaatggtc
     5641 gaccaaaaca cggtttccag gagtctcgat tatcttcaag gcgagatccc acgattgcgc
     5701 acgccgattt ctctcgggtg gagcttgctc ggtcttgcgg cctgggagtt gtggccgtcg
     5761 aacggcgcag ccctggtcga acgctgtttg gcaaatcaac tccgttatgg cgattacgat
     5821 acgtcggcgc tttgccttct cttcttgggc gcattagcag gagaatcgca cacggcgacc
     5881 cctctgttct tgcacggggg tcgtaatcag gcgccagcag tcatcggtca gtaaggagtg
     5941 ccgtgttaga tcacgtggat tccacatcga cgggaggcct tacgcgccgc gacgtgcttt
     6001 tggcagcgtc ggccggtctt gtgattggaa caggattgct tctcgtcccg aagcggtatt
     6061 tccgcaggcc gcaacgggcg caagcattcg tggctaaagc tgccaactac cagctggaca
     6121 ttgcggattt gatttcgagg gggattttgg agttgggtat tgctcctgag gaattgaagg
     6181 gcaaacgtat tctcttgaag ccgaatctag tcgagac
//
LOCUS       DATIFR010000204         5099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2823441, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000204 DATIFR010000000
VERSION     DATIFR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2823441"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2160
                     /locus_tag="VLD60_11850"
     CDS             <1..2160
                     /locus_tag="VLD60_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB45701.1"
                     /translation="LEWNLAQGSTIDVAVASQNVRLRVAGLIRSDEERESLWDRVVVM
                     DLAAAQVLFGSVGRLDRIELVTAADRTPEDVSSAVRTVLPPHLIVQRPAQRTAQVEKM
                     IGAFQLNLAVLSWVGLLVGMFLVYNTMSFSVAQRRREIGIYRALGMTERHVAGLFLVE
                     AGLFGLLGGLLGSLAGLWLARSLVLLVSRTISDLYVPLAAQGAAMTFDAHSVVALIQG
                     VVVGMAVSMAGALGPSIDASRTVTTKALAPGDYETTAQLRGRRYVWGSLALLILGGFC
                     TLGRPVAGLPIFGYVATLCLLGALSLLAPVCIVALRSVSQHRSPSSLALSGNLRHIAA
                     DHAARHPGRNGVTVSALIVGLAIMIGVVVMVRSFRETVEVWVNDTVMADLVIAPPAWL
                     HGKQVGQAARTLPFAWLPTLQATEGVAAVDTYRDVQVEAQGQPVALVSRDLRLHAQRS
                     RYLMVEGDSTSVLMRAAETGGVLLSEVLANRLSLRVGGTVMIVTPNGPKAVPVEGIFY
                     DYATDGGKMVMERTFYRQLWNDDRVTVFALYLEPAADSDQVRQSVARGLTRGQERLTP
                     PTIIRNKELRREILNIFDRTFVLTYVLEAIAVLVAVLGIVNTLVTAVLERRRELATLQ
                     AIGASTDQVERLVLWEAVYLGMIGAVLGVIGGLALAWVLIAVINKQSFGWTIRMTIPI
                     AVLFQAVLLAIAAAWLAGYFPARWAARQTVVDGLREE"
     gene            complement(2168..3163)
                     /gene="bioB"
                     /locus_tag="VLD60_11855"
     CDS             complement(2168..3163)
                     /gene="bioB"
                     /locus_tag="VLD60_11855"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSB45702.1"
                     /translation="MVVYQELANKALQDELLTRSECHAVMNAPDENLLELLQAAFAVR
                     SRYFGKTVRLQMLQNAKSGACQEDCHYCSQSAVSTAPIERYNLLPQKQMIEGARQAAA
                     SKAQRYCIVISGRSPLDREIDEIAGAVRAIKQEIPIQICCSLGLMNEQQAVRLKAAGV
                     DRVNHNLNTSEAFHSSICTTHTFQDRLSTIKNARAAGLEICSGGIVGMGEKDEDLIDL
                     AMALREIKPDSIPLNTLHPVAGTPLENCDNLTPQRCLKVLCLFRFLHPRTEIRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGAPAPEVWGMIEDLGFTIEVDYQQPVTK"
     gene            complement(3247..4647)
                     /locus_tag="VLD60_11860"
     CDS             complement(3247..4647)
                     /locus_tag="VLD60_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45703.1"
                     /translation="MLRLLTTIFLVSTGIFVYSYFRELNPGTITVHTGPSTQFELSAV
                     TLAVFSMAVGAVLVALAVGMRQTVHVIGNWRSTRLQRRKEKVDALYREGTHAFMSKRT
                     SEAVNLFEKALAIDPNRIDSLLWLGNIYRSESNYSEAIRLHQNAHRIDDRNIEILLEL
                     AKDLEGAKRYEDALQALHKILKIEPDNLTALIRKRDLLIRLEKWSDALEIQHRMAKAN
                     LPESERRAEAQLLTGCLYEVGRQLLERGHADKARRYFRGAIKKDRTFLPAYIGLGEIL
                     IQEGKTKQAVEILKKVFAKTRNVIILHRLEELFLEQGEPSEIIRTYQEALAQDPNNSI
                     LQFYLGKLYYRLEMVDEAFDILSTIEGPQDQLVDYHKIMANLFLRKQHMEQAVVELKK
                     ALQFKKRVVVPYVCTACQQETTEWSGRCRRCGNWNTFVALPWLEGGQASAGRDSSQLQ
                     PRTIPYQGIASPFETV"
     gene            complement(4652..>5099)
                     /gene="rsfS"
                     /locus_tag="VLD60_11865"
     CDS             complement(4652..>5099)
                     /gene="rsfS"
                     /locus_tag="VLD60_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSB45704.1"
                     /translation="TCTNKRTPIARTSKATALAIARAMLDKKATDVLVLHVAKLTSVA
                     DYLVLGSADSDRQTRAIADHVDGVLSQTGQGALSIEGKASSQWVLMDFGDVVVHIFRQ
                     DARHHYGLERLWADAKQIPIPGEEPAQPVIAQKPAPRVRTSAAKRA"
BASE COUNT          953 a         1366 c         1620 g         1160 t
ORIGIN      
        1 ttggagtgga atctcgcgca ggggagtacg attgacgtcg cagtggcttc tcaaaatgtg
       61 cggctgcggg tggcagggct cattcggagc gatgaggagc gggagtcgct gtgggatcgc
      121 gtggtggtca tggaccttgc ggccgcgcaa gtcctcttcg gatcggtcgg ccggctagat
      181 cgcatcgagc tggtaacggc cgcagatcga acgccagaag acgtctcctc tgccgtacgc
      241 acagtcctcc cgcctcatct cattgtgcaa cgaccggcgc agcgaaccgc gcaagtcgag
      301 aagatgatcg gggcctttca gctcaatctg gcggtcctaa gctgggtcgg acttctggtc
      361 gggatgtttc tggtctataa cacgatgagt ttctccgtgg cccaacgacg ccgggagatc
      421 ggcatctacc gtgcgcttgg catgactgaa cggcatgtgg ccggtttgtt tctcgtcgaa
      481 gccggattgt ttggcttgct tggaggattg ctgggcagcc ttgctggcct ttggctggct
      541 cggagtttgg tgttgctcgt cagccgtacg atcagcgatc tgtacgtgcc tctggccgca
      601 caaggagccg cgatgacgtt tgatgcgcac agcgtcgtgg ctctcatcca aggtgttgtc
      661 gtcgggatgg cggtatcgat ggccggtgcg cttggtccca gcatcgatgc gagtcgaacc
      721 gtgacgacca aagccctcgc gccaggagat tatgaaacga ccgcgcaact ccgagggcgg
      781 cgatatgtat gggggagtct cgcattgctg attcttggcg gcttctgcac gctcggtcgt
      841 ccagtggcag gattaccgat ctttggttat gtcgccacgc tttgcctgtt gggcgcgctc
      901 tcacttcttg cgccggtctg tatcgtggct ttgagatcgg tgagtcaaca tcgaagcccg
      961 tcgtctctgg cgctctcggg aaatctccgg cacatcgcag cagaccatgc cgctcgccat
     1021 ccaggccgta acggagtgac cgtttcagct ttgatagtgg ggttagccat catgatcggc
     1081 gtggttgtca tggtgcgaag ttttcgtgaa actgtcgagg tatgggtcaa cgataccgtg
     1141 atggctgatc tggtcatcgc cccgccggcc tggctgcatg ggaagcaggt aggccaggct
     1201 gcgcgcacac ttccttttgc atggttgccg acgctacaag ccacagaggg agtggcggct
     1261 gtggatacct atcgagatgt gcaggtcgaa gcgcaggggc aaccagtggc gctggtgtcg
     1321 agggatctcc ggcttcatgc gcaacggagt cgatacctga tggtggaggg cgattcgacc
     1381 agcgtcctca tgcgggccgc cgagacgggc ggggtgctgc tgtcggaagt cctggcgaat
     1441 cgactcagcc tgcgtgtcgg cggaacggtg atgatcgtaa cgccgaatgg gccgaaggcg
     1501 gttcccgtcg aaggtatctt ctacgactac gcgaccgacg gcgggaaaat ggtgatggag
     1561 cgaacgttct atcgccagct ctggaacgac gaccgagtga cagtctttgc gctgtatctc
     1621 gagccggctg cggacagcga ccaggtccgc caatccgtgg ctcgagggct caccagggga
     1681 caagagcgcc tgacgccgcc aacgatcatc cgaaacaagg aactccggcg ggaaatcctg
     1741 aatatcttcg accgtacatt cgtcttgacc tatgtgctcg aagccattgc ggtgctggtc
     1801 gcagtgctcg gcattgtgaa tacgcttgtg actgccgtgc tggagcggcg ccgtgagttg
     1861 gccacattgc aggcaatcgg cgccagtacg gaccaagtgg aacggttagt gctgtgggag
     1921 gctgtttacc tgggaatgat cggggcggtc ttgggcgtca tcggtgggct tgcgctggcc
     1981 tgggtgctga tcgccgtcat caataaacag tcattcggct ggacgatccg catgacgatt
     2041 ccgattgcag tgctcttcca agccgtgctg ctcgcaatcg ctgcagcctg gctcgccggc
     2101 tactttccag cacgttgggc agccagacaa accgttgttg acggcctgag agaggagtag
     2161 aagagaacta cttggtcaca ggctgctggt aatccacttc gattgtaaag cccaaatctt
     2221 cgatcatccc ccagacttcc ggtgccgggg cgccgggtgt cgtgaggtag ccgttcacga
     2281 agaccgaatc ggccggatag agggccaacg gttgcaggct gcgcaggttg tgctctcggc
     2341 ctcccgcgat gcggatttcc gttcgcggat gaaggaagcg gaagaggcag aggactttca
     2401 aacagcgttg cggcgtgagg ttgtcgcaat tctccaaggg ggtgcctgcg accggatgca
     2461 aggtattcag cggaatcgag tccggtttga tttcgcgcaa agccatggcg agatcgatga
     2521 gatcttcgtc cttttcgccc atgccgacga tcccacccga gcaaatttcc aatcccgccg
     2581 cacgtgcgtt cttgatggta gacagtcgat cctgaaacgt gtgggtggtg caaattgacg
     2641 agtggaacgc ctcgctcgtg ttcaagttgt ggttcacgcg atcgacaccg gcggctttca
     2701 gccgcacggc ttgctgctcg ttcatcaaac cgagcgaaca gcagatctga atcggaattt
     2761 cctgcttgat agcacggacg gcgccggcga tctcgtcgat ctcgcgatcg agcggacttc
     2821 tgccgctgat gacgatgcaa tagcgctgag ccttggaagc agcggcttgg cgtgctcctt
     2881 cgatcatctg tttctgcggc aataagttat agcgttctat cggtgcggtt gagacagcgg
     2941 attgcgagca gtagtgacag tcttcctggc aggcgccgct cttggcgttc tgcaacatct
     3001 gcaggcggac ggtttttccg aaatagcggg atcgcacggc aaaggcggcc tgcagaagtt
     3061 cgagaagatt ctcatcgggg gcgttcataa cggcatggca ttctgaacgg gtgagcagtt
     3121 catcctgcag ggctttgttg gcaagttcct gatacacgac catcggaggc tccttcactc
     3181 atcacgctgt cgcgaggtga tcacgcagca ccggtttctg tcacattgtc tgtatgagcg
     3241 gaaaacctac acggtttcaa acggagaagc aatgccttgg taggggattg tgcgaggttg
     3301 cagctggctg ctgtcccgtc ctgctgatgc ttggccgcct tccagccagg ggagtgcgac
     3361 gaaagtgttc caattgccgc agcggcggca gcgtccggac cattccgtcg tttcctgctg
     3421 gcaggccgtg cagacgtagg ggacgacgac acgcttcttg aattgcaacg ccttcttcaa
     3481 ttcgacgacg gcctgttcca tgtgctgctt gcgtaagaat agattcgcca tgatcttgtg
     3541 atagtcgacc aactggtcct gaggtccttc gatggtggag agaatgtcga aggcctcgtc
     3601 gaccatctcc aggcgatagt agagcttccc caggtagaac tgcagaatgg agttgttcgg
     3661 atcctgcgcc agggcttcct gataggtgcg gatgatctcg ctcggctcgc cctgctccag
     3721 gaagagttcc tcgagccggt ggagaatgat tacgttacgg gtcttcgcga agaccttttt
     3781 caaaatttca acagcttgct tggtcttgcc ttcctggatg aggatctcgc cgagtccgat
     3841 ataggccggg aggaaggtcc ggtctttctt gatggcgcca cggaaatagc gcctggcctt
     3901 gtcggcgtgc ccgcgttcga ggagctggcg gccgacttca tacaggcagc cagtgaggag
     3961 ctgagcttcg gcacggcgtt ccgattccgg aagattggcc ttggccatgc gatgctggat
     4021 ttcgagcgcg tcgctccatt tctcgaggcg aatcaaaaga tcgcgcttgc gaatcagcgc
     4081 cgtgaggttg tccggctcga ttttcaggat cttgtggagc gcttgcaggg catcctcata
     4141 ccgtttggcg ccctcgagat ctttcgccaa ttccaagagg atctcgatat tgcggtcgtc
     4201 gatgcgatgg gcgttttggt gcaatcggat ggcttccgaa taattgctct cggaccggta
     4261 gatgttgccc aaccagagca aagagtcgat tcgattggga tcgatggcga gggctttttc
     4321 gaagaggttg acagcctcgc ttgtgcgctt cgacatgaag gcgtgggtac cttcgcggta
     4381 cagcgcgtcg actttttctt tccgccgttg aagccgcgtg ctgcgccaat tgccgatcac
     4441 gtgcacggtc tgccgcatgc cgaccgccag cgccacgagc acggccccga cggccatgga
     4501 gaagacggcg agggtgaccg cgctcaattc gaactgcgtt gaggggcctg tatggacggt
     4561 gatagtgccg ggattcagtt cgcggaaata actgtagacg aagattccgg tgctgacgag
     4621 gaagatcgtg gtaagcagtc tgagcattgc attacgctcg tttggctgcg ctggtcctta
     4681 ctcgcggggc cggcttctga gcaattaccg gctgagccgg ctcttccccg ggaatgggaa
     4741 tctgtttcgc atctgcccac aatcgttcga ggccataatg gtggcgcgca tcctgccgga
     4801 aaatgtggac gaccacatcg ccgaagtcca taagcaccca ctgtgacgat gctttgcctt
     4861 ctatgctgag ggctccctgt ccggtctggg acagtacacc atcaacgtga tcggcaatgg
     4921 ccctggtttg ccggtcggaa tctgcggaac ccaggaccag ataatcggca acagaggtca
     4981 gtttcgcgac atggaggacg aggacgtctg tggccttctt gtcgagcatg gccctcgcga
     5041 tagcaagggc tgtggccttc gatgttcgtg cgatcggtgt cctcttattc gtacaagtg
//
LOCUS       DATIFR010000205         2756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1865672, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000205 DATIFR010000000
VERSION     DATIFR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865672"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..371)
                     /locus_tag="VLD60_11870"
     tRNA            complement(285..371)
                     /locus_tag="VLD60_11870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Leu,seq:cag)
     gene            complement(432..1904)
                     /locus_tag="VLD60_11875"
     CDS             complement(432..1904)
                     /locus_tag="VLD60_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45705.1"
                     /translation="MTARDLIEIWASRLRAEQEHLMEAGLDQPIAPVTGRLVQMVGTL
                     YLYEFRLPAGRSLEIDTPLSIIPADAMEPTEGVALSCRGDLAVVQTFDTIGQTIEGAT
                     IIPDRGGFLTTSAIRLTEMLARADAYRLGPADRLAPLLEASGGFEDSGVGAASILTTI
                     WSDDASMRRQRIASMAIELIRANKRILLVCPDHHAADEVAGTIARAMKAGGLLYKTWI
                     SRYEMTLAQQASGLAIHELGFEAQMHQFYAKSRADKAALRRKYERFRELTPLLAYKGQ
                     KQKDLDEVRLLEWRLLTQLSDLQAKTKEANATLAEYESLPLLRRLSMQAVGKNVESLH
                     QYLALYESQIAALMKELDIAKARIAELVPEAAVPKDMRPEFEELKEDIVRLGGTKKIR
                     ELLAAEEDTNRQAFIQNRRLVITTAARVLGDPLFSRVRFDVLIADSAPWISAPALLGS
                     AGLVRERIVLTGDTRDIAGAGLWTSSELSAGIRTKDESLA"
     gene            2092..2559
                     /gene="rplI"
                     /locus_tag="VLD60_11880"
     CDS             2092..2559
                     /gene="rplI"
                     /locus_tag="VLD60_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSB45706.1"
                     /translation="MKVILQETMEGVGHLGDLIDVKDGFARNFLLPRKKAVVANDRNI
                     KSFEHAKRVAAERAKKEKLEIEAHAKKMSTVTLTVEAQVGKDDKMFGSVTVKDIAEGL
                     AEQGYQVDRRKIQLPQPIKELGSFTVPVKLPREVTAAVTVRVVKKQEAETASA"
     gene            2575..>2756
                     /locus_tag="VLD60_11885"
     CDS             2575..>2756
                     /locus_tag="VLD60_11885"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSB45707.1"
                     /translation="MSMKDAVGSLDALQATDPEVYAAIDAEEERQRNKLLLIASENFA
                     SPAVLAAQGSLMTNKYA"
BASE COUNT          603 a          785 c          785 g          583 t
ORIGIN      
        1 cgctgctctt tttcaccgat ggtcggagca ttacgattta agacctgcgt gctcgagcgt
       61 cggccgccca cccgctcctt ccactcgcga gacatgatat ctgacctgcg agtcagtacc
      121 gagatccgag ggaatttaga accgcgttct gcccccctag gcaggctcac ttgacaatta
      181 cttgacaatt cagcccaaaa acacgccgtg gcaggtgtta atgggtgtta gaaacgaggg
      241 ctctaaaacg ctggaaaggc ttgatagggg cggagaatgt ggtgtggtgc cgaagccggg
      301 atttgaaccc ggacaccctt gcgggcgcta gaccctgaat ctagtgcgtc tgccagttcc
      361 gccacttcgg cataggaggc tgctgatgaa acgtcaggga gccggattat cgtgaattcg
      421 gatcgtccgc gtcaagcaag gctctcgtcc ttagtgcgga ttccggctga tagttcagat
      481 gaagtccaga gtcctgcgcc ggcgatgtct ctggtatcac ccgtcaacac aatccgttct
      541 cggacgagtc ccgccgatcc gagtaaagca ggcgctgaga tccatggcgc gctgtcggct
      601 atgagcacgt cgaaccggac gcggctgaat aacggatcgc cgaggacgcg tgccgccgtg
      661 gtaatgacca gccgccggtt ctggataaag gcctgccgat tggtgtcttc ctcggcagcc
      721 agcagttctc gaatcttctt cgtgccgccc aatcgaacga tgtcctcttt caattcctcg
      781 aactccggcc tcatgtcttt cggaacggcc gcttccggca ccaattcagc gatgcgggct
      841 ttcgcaatat caagctcctt catgagggcg gcgatctgac tctcatagag ggcgaggtac
      901 tggtggagcg actcgacgtt tttcccgacg gcctgcatgc tcagacgacg taacaatggg
      961 agactctcgt actctgccag cgttgcgttg gcttctttgg tcttagcttg cagatcgctc
     1021 agttgtgtaa gcaaccgcca ctctagcaga cgcacttcgt cgagatcctt ttgcttctgt
     1081 cctttatagg ccaagagcgg cgtgagctcg cgaaagcgtt catacttgcg ccggagcgcc
     1141 gctttgtctg cacgtgactt ggcataaaac tgatgcatct gagcctcaaa ccccaactcg
     1201 tgaatggcaa gccctgaggc ctgctgtgcc aacgtcatct catagcggct aatccaggtc
     1261 ttatacagga gtcctccggc tttcatcgct cgcgctatcg tgccggccac ttcgtccgcc
     1321 gcatggtggt ccggacagac cagaaggatg cgtttgttcg cacggatcag ctcgatcgcc
     1381 attgatgcaa tccgctggcg ccgcatcgaa gcatcgtccg accaaatggt cgtcaggatg
     1441 gaagcagcac ctaccccgga atcttcaaat ccaccggaag cctcgaggag aggagccaac
     1501 cggtcggctg gaccaagtcg gtaggcatcg gctcgggcca gcatctccgt cagtcgtata
     1561 gccgaggtgg taagaaatcc cccgcgatcg ggaatgatcg tggcaccttc gatcgtctgg
     1621 ccgatggtgt caaaagtttg tacgacggct agatcgcctc ggcaactcaa ggcaacacct
     1681 tccgtcggtt ccatagcgtc agcaggaata atcgagagcg gcgtatcaat ctccaatgat
     1741 cggcctgccg gaagcctgaa ttcatagaga tagagcgtcc caaccatctg caccaagcgc
     1801 cccgtcacag gggcaatcgg ttggtcgaga cccgcttcca tcaaatgctc ttgttcagcg
     1861 cggagccgac tggcccagat ctcaatgagg tctcgagctg tcatccctat ccccgattga
     1921 agtagttcgg aggccatcat acgggctgac cgtagcgaca gtccagtcca tcgctgtcgg
     1981 tttcaaccag gattcatggt gaatattgac accgtccgca gaagatcgga attgactttt
     2041 cgccctcccc ccagtatcct cttttccttt ccaacagaag gagttagggg tatgaaggtc
     2101 attctacaag agacgatgga aggggtcggc cacttgggcg atctcatcga tgtgaaggat
     2161 gggtttgccc ggaattttct cctcccgcgg aagaaggctg ttgttgccaa cgatcggaac
     2221 atcaagtcct tcgagcatgc gaagcgcgtg gcggcagagc gggcgaagaa agagaagctc
     2281 gaaattgagg ctcacgcaaa gaagatgtcc accgtgacgc tgaccgtcga ggcgcaagtc
     2341 ggtaaggacg acaagatgtt cgggtcggtc acggtaaaag acattgccga aggacttgcg
     2401 gagcaggggt atcaggtgga tcgccgcaag atccagctgc ctcaaccgat caaggagctc
     2461 gggagtttca cggttccggt caagctcccg cgtgaggtca ccgccgctgt aacggtgcgc
     2521 gtagtcaaga agcaagaagc cgaaacggct tccgcatagg aacgacgagg ccctatgtct
     2581 atgaaagacg ctgtcggctc tctcgatgcg ctacaggcga ccgatccgga ggtctatgct
     2641 gccatcgatg ccgaagaaga acggcaacgc aacaagctcc tgctgatcgc ctcggaaaat
     2701 tttgccagcc cggcggtctt ggcggcacag ggctccctga tgacgaacaa gtacgc
//
LOCUS       DATIFR010000206         7514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1865773, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000206 DATIFR010000000
VERSION     DATIFR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865773"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="murJ"
                     /locus_tag="VLD60_11890"
     CDS             complement(<1..980)
                     /gene="murJ"
                     /locus_tag="VLD60_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSB45708.1"
                     /translation="MSEPTTAPPDKPQHENHSVVKAAGVIGIATFSSRILGFIRDMVL
                     ARLFGATPAADAFFVAFRIPSLLRELFAEGSMSSAFIPVFTEYHTLRSKREAWELASA
                     AFTTLLTIVTLVTLLGIVAAPWLVQALAPGFQSSPEKLALTTVLTRVMFPYLLFISLA
                     AVAMGILNSVRAFAAPAYAAVFLNVFIIGCALFLSPQLPEPIVGVAIGVVAGGAAQFA
                     MQLPSLKLRGLLFGLRFQLGHPGVKRIGQLMVPSLLGMSVTQINLTVSTILASFIAGA
                     PTYLFYGMRLIQFPLGIFGVALAMAILPTLSAQAARGALEELRTTLGFGLR"
     gene            complement(1030..1692)
                     /locus_tag="VLD60_11895"
     CDS             complement(1030..1692)
                     /locus_tag="VLD60_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSB45709.1"
                     /translation="MEIGSRVELTAAERERIRLAVHAAEQRTNAEIVPMIVARSGIYR
                     EARHRMGLALALVVLTVMLTIDSQWLPWGWHASNAAWLLLATLTAYGCGTWLGTLWPV
                     IRLWTSAERMRHKVRVRAERAFSQHAISQTRERTGVLILLSILERQIYVLPDRSLAGL
                     ASTDQWGQVVRAAVDRLTHGDIVSGLCQGIEQSGVLLADVCPGRPGDSPNELPDTVIQ
                     EP"
     gene            complement(1679..2560)
                     /locus_tag="VLD60_11900"
     CDS             complement(1679..2560)
                     /locus_tag="VLD60_11900"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSB45710.1"
                     /translation="MSRVWREPARLTFFLCLLLGPASAWALDVPTLTGRVVDLAHVLP
                     APTVEAVTAQLKVHEETTGNQVAVLVLPSLEGEPLESFSHRVATTWKLGQKGTDNGVL
                     FMVAMKERKIRIEVGYGLEGTLTDARSAQIIRNEIVPRFRAGDVPGGVAAGVDAILKT
                     IEGTYQVSDKAPPGRDSDNMGQVVMAVIIGLVVGLGFMSVHRLLGPVAGTGLSMLLAP
                     WLVPALITGGITLLLLIFFIAAGAGGRGRGRHGGDDWVWYSSRSGGWGGGIFGNDTGG
                     FGGGGGDFGGGGASGNW"
     gene            complement(2589..3191)
                     /locus_tag="VLD60_11905"
     CDS             complement(2589..3191)
                     /locus_tag="VLD60_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSB45711.1"
                     /translation="MGRRVSVFAACLAAFIYAGLSGCGYNDLQGLDEDTKAAWSEVVN
                     QYQRRADLIPNLVATVKGYAAHEKDTLEGVVQARAQATGIQVTPELLKDPVAFEQFQK
                     AQAGLTSALSRLIAIAENYPNLKADQNFRDLQSQLEGTENRIAVARKRYIDRVAEYNK
                     MVRFFPTNLTAKFLLHMEEKPNFTVADEKAVTTPPEVKFN"
     gene            3293..3598
                     /locus_tag="VLD60_11910"
     CDS             3293..3598
                     /locus_tag="VLD60_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45712.1"
                     /translation="MSLEPSSRLPGGFQLRHRTLGLYQGSAIELAFWHPSSGMPEYGL
                     CRFSTQEKAQSMIEFLTSPACAEPLNREDLTIEPFDLVEHERLTTEHPLPSAWEQPT"
     gene            complement(3657..4181)
                     /locus_tag="VLD60_11915"
     CDS             complement(3657..4181)
                     /locus_tag="VLD60_11915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB45713.1"
                     /translation="MGRLLNCPQCHHERVLRSRPLSASERMAALFLLSPFSCPHCSCR
                     FMASRIGLDYPRHPMDRREHLRIPVRLFLSFSGGKVRGEGTVLDLSMGGCIIKSQTQV
                     HVDDIFYLEISTGQQDQPLEVAAMVRSVGPRGIAFKFLRAAQENKRLLAFIQAQTADH
                     LQKAAAPSEVPVKS"
     gene            complement(4395..5186)
                     /locus_tag="VLD60_11920"
     CDS             complement(4395..5186)
                     /locus_tag="VLD60_11920"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HSB45714.1"
                     /translation="MKLTLKIWRQEGPEKQGRLVTYRAPDVSPDMSFLEMLDVVNQQL
                     ILKREEPIAFESDCREGICGSCSLVIDGIPHGPERGITACQLYMRRFTDGATITIEPW
                     RAKAFPVIKDLVVDRSALDRMMQAGGYVSVNTGGAVDGNVLLIGKDRAETAMDAASCI
                     GCGACVAACKNASAMLFVAAKVSHLASLPQGEPERNRRAKAMVVAMDREGFGTCGNQY
                     ECEAVCPKSISVRFIAKLNREFLLATIAERAPHIESPAAPHDESH"
     gene            complement(5239..7152)
                     /locus_tag="VLD60_11925"
     CDS             complement(5239..7152)
                     /locus_tag="VLD60_11925"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HSB45715.1"
                     /translation="MIVLDSKVPSGPLETKWDRHRFSEKLVSPSNKRRINIIVVGTGL
                     AGASAASTLGQLGYHVDCFCFQDSPRRAHSIAAQGGINAAKNYQDDGDSVTRLFYDTI
                     KGGDFRSREANVYRLAQVSTQIIDQCVALGVPFAREYGGHLANRSFGGVQVSRTFYCR
                     GQTGQQLLMGAYSALCWQIERGQVTMHPRTEMLDLIVVDGHARGIVVRNLVSGAITVH
                     LAQAVVLATGGYSNIYFLSTNATGSNVTATYRAWKQGAAFANPSFTQIHPTAIPPTEE
                     YQAKLTLMSESLRNDGRLWVPKKPGDARSADRIPSSERDYFLERRYPRFGNLVPRDVA
                     SRAVKQLCDEGRGVGPGGHGVYLDLADAIAQEGLEVVRERYGNLFDMYQRITGEDAYR
                     APMRIYPAPHYTMGGLWVDYNLMSTIPGLFVIGEANFADHGANRLGASSLMQGLADGY
                     FILPYTIGHYLATTKGEPISHGCAEVRAAIERVKDRIARLLEGKRKRTAGSFHRALGH
                     IMWNACGMTRHRAGLKHALQTIPPLRYEFWRDVAVPGSGETFNQELEYAGRVADYLEF
                     AELLCHDALHREESCGAHFREEHQTADGEPLRDDRNFSHVAAWEYVPSGQPALHREPL
                     VFEFVKPSTRSYQ"
     gene            complement(7149..>7514)
                     /locus_tag="VLD60_11930"
     CDS             complement(7149..>7514)
                     /locus_tag="VLD60_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45716.1"
                     /translation="RTMALTGGLLLLFIIFHLLHLTLGVVDSSFTDRLDAHGRRDVFS
                     KVIHAFQNPLFVVLYLAGQMVLGLHLSHALSSSLQTLGIEHPILDRLFRGAGPVVALL
                     VVAGNMAIILAVALGMVRA"
BASE COUNT         1595 a         2329 c         2127 g         1463 t
ORIGIN      
        1 cgcaaaccga atccgagcgt cgtccggagt tcctccagcg ccccgcgcgc cgcctgcgcc
       61 gacaacgtcg gcaggatggc catcgccagc gccacgccga agatgcctaa gggaaattga
      121 atcagccgca tgccatagaa cagataggtt ggggcgcctg cgataaacga ggccaggatc
      181 gtgctcacag tcaaattgat ctgcgtgact gacatcccaa gcaaggacgg caccatcaac
      241 tggccgatcc gcttcacacc aggatggccg agctggaatc gcaaaccaaa caataaccct
      301 ctcagtttca gactggggag ctgcatggca aactgcgccg cccctcctgc aaccacgccg
      361 atcgcgacgc cgacaatcgg ctccggcaat tgaggcgaca gaaacaaggc acagccgatg
      421 atgaacacgt tgaggaatac tgcggcgtag gccggagccg cgaacgcccg cacggaattc
      481 agaatgccca tggcgaccgc ggcaaggctg ataaacagaa gataggggaa catcacgcgc
      541 gtcaggacgg tcgttaacgc caatttttcc ggggacgact gaaatcccgg cgccagggct
      601 tggaccaacc agggtgccgc aacaatgccg aggagagtca ccagcgtcac gatcgtgagc
      661 aaagtcgtaa acgccgcact ggccagctcc cacgcttccc gcttggacct gagcgtgtga
      721 tattccgtaa agaccggaat aaacgcagag gacatggagc cttccgcgaa cagctcgcgc
      781 agcaagcttg gaatgcggaa cgcgacaaag aaggcgtcgg ccgccggtgt cgcaccgaag
      841 agtctggcca ggaccatatc gcggataaat cccaggatgc ggctggaaaa tgtggcaatg
      901 ccgatcacgc cggccgcttt cacgaccgaa tgattctcgt gctggggttt gtcaggaggt
      961 gcggtggtgg gttccgacat gaatccggat tctaacggaa cgataccggg cagtctatcg
     1021 aactgcactt tacggttctt gaatcaccgt atccggcaat tcgttcgggc tgtcaccggg
     1081 gcggccgggg cagacatccg caagcagcac gccgctctgt tcgatcccct ggcataaccc
     1141 gctgacgatg tcaccgtgcg tcaaccgatc gacggcggct cggacgacct gcccccattg
     1201 atcggtcgaa gcgagtccgg ctaatgatcg atccggtaac acatagatct gccgttccaa
     1261 aatcgacaga aggatgagca cgcccgtgcg ctcacgcgtc tgcgaaatgg catgctggga
     1321 aaacgcgcgc tccgctctga ctcttacttt gtggcgcatc cgctcggcgg aggtccacag
     1381 acgaatgacc ggccacaagg ttcccagcca tgtgccgcaa ccataggcag tcagcgtcgc
     1441 gagcaggagc caggccgcgt tcgaggcatg ccagccccag ggaagccatt gcgaatcaat
     1501 cgtcagcatc acagttaaga ctacgagcgc gagggcgagc cccatccggt gtcgagcctc
     1561 gcgatagatt cctgagcgag ccacaatcat cgggacgatc tccgcgttcg tgcgctgctc
     1621 cgccgcatgc acggccaggc ggatccgctc ccgttccgct gcggtcaact ccaccctgct
     1681 accaatttcc actggctcct cctcctccaa aatcgccccc gcctccacca aatccgccag
     1741 tatcgttgcc aaatatgcca ccgccccatc cgccacttcg actgctgtac caaacccagt
     1801 cgtccccgcc gtgtcgcccg cgtcctctcc cccccgcacc ggctgcaatg aagaatatga
     1861 gcagcaatag ggttatccct ccggtgatca gggcaggcac cagccaaggc gccagaagca
     1921 tggagagtcc tgtgccggcg accggaccga ggagccgatg aacactcatg aagcccaatc
     1981 caacgactaa gccgataatc actgccatca cgacctgtcc catattgtcg ctgtcccgcc
     2041 ccgggggcgc cttgtcggaa acctgatagg tcccttcaat cgtcttcaga atcgcatcaa
     2101 cccccgcggc aaccccgcct ggcacatcgc cggcgcgaaa ccggggaacg atctcatttc
     2161 tgatgatctg cgccgaccgc gcatcggtca atgtaccttc caatccatag ccgacttcga
     2221 tgcgaatctt gcgttccttc atggcaacca tgaacaggac gccgttgtcg gtcccttttt
     2281 gacccaattt ccaggtcgtt gcgacgcggt gggaaaaaga ttcgagcggt tcaccttcga
     2341 gggatggcag cacaaggacg gcgacttggt tgccggtggt ttcttcgtga accttgagtt
     2401 gtgcggtgac ggcttcgacc gtgggggccg gcagcacgtg ggcgagatcg acaacgcggc
     2461 cggtgagcgt ggggacatcg agggcccagg ccgatgcggg ccccagcaac aggcagagaa
     2521 agaacgtgag ccgcgccggc tctcgccaga cacggctcat gctgatggca tccatcgtga
     2581 gcctgcgttc agttgaattt cacctctggt ggtgtcgtca cggccttctc atctgcaacc
     2641 gtgaagttcg gtttctcctc catgtgcagg aggaattttg ccgtgagatt cgtggggaag
     2701 aatcggacca ttttattgta ctcggcaacg cggtcgatgt agcgcttacg ggcaaccgcg
     2761 atgcggttct ctgtaccttc taactggctc tggaggtcac ggaaattctg gtcggctttc
     2821 aagttcggat agttttccgc aatagcgatc agacgactca gcgccgaggt caggcccgct
     2881 tgagccttct ggaactgctc gaacgccaca gggtctttca acaactccgg cgtcacctga
     2941 atgccggtcg cctgcgcgcg ggcttgcacc accccttcca gtgtatcctt ctcatgcgcc
     3001 gcatagcctt ttactgtggc caccaggttc gggatgagat cggcccgccg ctggtactga
     3061 ttgacgactt cgctccaggc cgctttggtg tcttcgtcca aaccctggag gtcgttataa
     3121 ccacagcctg agagtcccgc gtagatgaac gccgcgagac atgctgcgaa taccgagacg
     3181 cgtcgaccca ttgccgtccc tccttctctg atccatccgg cacgttgtgc cggccggtgc
     3241 cattcatgct accataggca acattccgag gatacggcgc tgcaaggatc cgatgtcgct
     3301 tgaaccgtca tcgcgtttgc cggggggatt tcagctcagg catcgcacgc tcggcctgta
     3361 ccaagggagc gccatcgaat tggccttctg gcatccttcc tccggcatgc cggaatacgg
     3421 actgtgccgc ttctccacac aggaaaaggc gcagtctatg atcgagtttc tcacctcgcc
     3481 ggcctgcgcg gaacccctaa acagagagga tctgacgatc gagccgttcg atctcgtcga
     3541 gcacgagcga ttgacgacgg aacatcccct tccctccgcc tgggaacaac cgacctgatc
     3601 tgtataggtg ccagagagat gagggagaga gcggctcttg aagagagacg attgagctag
     3661 gatttgaccg gcacttcgga aggagcggca gccttctgaa gatgatcggc ggtttgagcc
     3721 tggatgaacg cgagcaagcg tttgttttct tgggccgcgc ggagaaactt gaacgcgatc
     3781 ccccgtggac cgacggaacg caccatcgcg gcgacttcca atggctgatc ctgctggcct
     3841 gtggaaatct ccaagtagaa gatatcgtcg acgtgcacct gagtctgact cttaatgatg
     3901 cagccgccca tcgacaggtc caagacagtg ccttctccgc gcacttttcc tcccgagaac
     3961 gagagaaaaa gccgcacagg aattctgagg tgttcgcgcc gatccatcgg gtgacgagga
     4021 taatctaatc cgatgcggga ggccatgaaa cggcaactgc aatgcggaca ggagaacggc
     4081 gataagagga aaagtgcggc catgcgctcg ctcgccgaca gcggccgcga ccgcaacacc
     4141 cgctcatgat ggcattgtgg acagttaagg agtcgcccca tctcgtctga ttccgcgtct
     4201 atccgtcgcc ctacgaattc gtaaccaatc acaagtcggc ttccagagcc gatttatccg
     4261 gaccaggatt ctcttcatcg atagtgcaag accgcagatc tgtcaaggaa acaccttgtt
     4321 tcaacttcgc tgccggacca agactcaccg gctcggaagt ttcgtgagac tcggacgacc
     4381 aacaaggcgg tctgtcagtg agattcatcg tgtggagcgg ctggcgattc gatatgaggc
     4441 gcccgttcgg caatggtcgc gagaagaaat tctcggttga gcttggcaat aaaccgtacg
     4501 ctgattgatt tggggcagac cgcctcgcac tcgtattgat tcccgcaggt tccgaaccct
     4561 tctcgatcca tagcgacaac catggctttg gcgcgcctgt ttcgctctgg ctctccttgt
     4621 ggcaaagagg ccaggtgcga caccttggcg gcaacaaaca gcatggccga cgcattcttg
     4681 caggcggcca cgcaggcgcc gcagccgata caggacgcgg catccatggc ggtttccgcc
     4741 cggtccttgc cgatcaacaa gacattcccg tcgaccgccc cgccggtatt gaccgaaaca
     4801 tatccgcccg cttgcatcat gcgatcgagc gcactccggt cgacgacgag atccttgatg
     4861 accggaaatg ccttggctct ccatggttca atggtaatcg tcgcgccgtc ggtaaaccgc
     4921 cgcatgtaga gttggcaggc ggtgatccct cgttcgggcc cgtgggggat gccatcaatg
     4981 accagcgagc aactcccgca aatcccttct cgacaatcag attcgaatgc gatcggctct
     5041 tctcgtttga gaataagctg ttgattcaca acgtccaaca tttccaggaa ggacatgtcg
     5101 ggactcacgt ccggagcccg ataggttacc aatcgaccct gtttctcagg accctcttgc
     5161 cgccaaatct tcaacgtcaa cttcataatt cgcgccccaa cctctcgctt gttctctctg
     5221 ctccgtgtcc ttcgccgatc attgatagct tcgcgtcgaa ggtttcacga attcgaacac
     5281 gagcggttcc ctgtgcagcg ccggctggcc gctcgggaca tactcccagg ccgcaacatg
     5341 ggaaaagttt cggtcgtcgc gaagcggctc cccgtccgcc gtttgatgct cttctcgaaa
     5401 atgggcgccg caagactctt cgcggtgaag tgcgtcatgg cacagaagtt cggcgaactc
     5461 cagataatcc gcaacccgcc ccgcatattc caactcctgg ttgaatgttt caccagagcc
     5521 cggcacggcc acgtcacgcc agaactcata gcgcaacgga gggatcgtct gcagtgcgtg
     5581 tttgaggcct gcgcggtgtc gcgtcatgcc gcaggcgttc cacatgatgt gccccagtgc
     5641 ccgatggaat gacccggccg ttctcttcct tttcccttca aggagtcggg cgatcctatc
     5701 cttcacgcgc tcgatcgccg cccggacttc cgcacagccg tgtgagatcg gctcgccctt
     5761 cgtcgtcgca agataatggc cgatcgtata tggaagaatg aaatagccgt ctgccaaccc
     5821 ctgcatcagc gaacttgcgc ccaaccggtt cgcgccgtga tcggcaaagt tggcttcgcc
     5881 gatcacgaag agacccggaa tcgtactcat caagttgtaa tcgacccaga gaccgcccat
     5941 cgtgtagtgc ggtgcgggat agatgcgcat cggcgccctg tacgcgtcct ctccggtgat
     6001 ccgctggtac atgtcgaaca gatttccgta gcgctcgcgc actacctcaa gcccctcctg
     6061 cgcgatggca tcggcaagat ccaagtagac tccatgtcca ccgggaccga caccgcgccc
     6121 ttcgtcacag agctgcttga ccgcgcggga ggccacgtct cgcggcacca ggttgccgaa
     6181 gcgagggtac cgtcgctcca ggaaatagtc ccgctccgac gagggaatcc gatcggccga
     6241 cctcgcatcc cccggttttt tcggaaccca caagcgtccg tcgttacgga gcgattcaga
     6301 catgagcgtg agcttggctt ggtattcctc agtcggcgga atcgcggtcg gatgaatctg
     6361 cgtgaacgaa gggttggcga aggccgcccc ctgcttccag gcccggtagg tggcggtgac
     6421 gttgctgccg gtggcattcg tcgagaggaa atagatgttg ctgtacccgc ccgtcgccag
     6481 caccacggcc tgtgcgaggt gaaccgtgat agcccccgag accaggttgc gcacgacaat
     6541 cccgcgggca tggccgtcga cgacaatcag gtccagcatc tcggtacgcg gatgcatcgt
     6601 gacttggccg cgctcaatct gccagcagag cgccgagtag gcacccatga gcaattgttg
     6661 ccccgtctgg ccgcgacaat aaaacgtgcg agagacctgc acgccgccga aggaccggtt
     6721 ggccagatgc cctccgtatt cccgggcgaa ggggacaccg agtgccacgc actgatcgat
     6781 gatctgagtg ctgacctgtg ccagtcggta cacgttcgct tcgcgggagc ggaaatctcc
     6841 tcctttgatc gtgtcataaa agagccgagt gacgctgtct ccatcatcct ggtaattctt
     6901 cgccgcattg atgccgcctt gtgccgcaat gctatgggca cgacgagggc tatcctgaaa
     6961 acagaaacag tctacgtgat agcccaattg ccctaacgtg gaggcggcgc tggcaccggc
     7021 cagaccggtg ccgacgacta tgatgttgat gcgccgcttg ttgctcggac tcaccagttt
     7081 ttcgctgaac cgatgacgat cccacttcgt ttcgagcggt cccgaaggga ctttcgagtc
     7141 gagtacgatc atgcgcgcac cattccaagc gccaccgcca agatgatggc catgttgccc
     7201 gccacgacca gcagtgccac caccggacca gctcctctaa agagacggtc gaggatcgga
     7261 tgttcgattc ccagcgtctg cagactgctg gacagcgcat gactgagatg gagccctagc
     7321 accatttgcc cggcgaggta cagcacgacg aacagcggat tctggaacgc atgaatgacc
     7381 ttgctgaaca cgtcccgacg tccgtgcgca tccagtcgat ccgtaaaaga ggagtccacc
     7441 acgccaagag taaggtgtaa gagatggaag atgatgaaca gtaggagcaa gccgcctgtc
     7501 agggccatgg tgcg
//
LOCUS       DATIFR010000207         6309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1866315, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000207 DATIFR010000000
VERSION     DATIFR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866315"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VLD60_11935"
     CDS             <1..620
                     /locus_tag="VLD60_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB45717.1"
                     /translation="AGKTTAMNLIHRFYDPTEGRITIDGLDLRQVTLESWYRQIALVP
                     QETILFGGTILDNIRYGNREASEEMVEAASHAAHAHDFIMSFPDQYHTVVGEKGINVS
                     GGQRQRIAIARAILKNPRILLLDEATSALDSESERLVQEALEQLMKGRTTFVIAHRLT
                     TIQRADRILVLNKGRLVETGTHSELMDRQGLYHYLYTLRLTELPS"
     gene            734..1003
                     /locus_tag="VLD60_11940"
     CDS             734..1003
                     /locus_tag="VLD60_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45718.1"
                     /translation="MTDCDALELAILERVASGAPLSFEDLQQFLRHHSWNRLFAAVDQ
                     LSRKGSLAIRRIDRCTYHISLGPQFATQASGIQTDNASTSVTTQS"
     gene            1202..1693
                     /locus_tag="VLD60_11945"
     CDS             1202..1693
                     /locus_tag="VLD60_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45719.1"
                     /translation="MHSTRSFILSLCFMVAAVGLFTAANSFAADGHTTSKFEGVKANS
                     GTATHARSGNNDTLTWSDDFKIPETPAPHWQVIDTKGNVYLLNRLKIKGGVLGGEKEN
                     RTITIPSYIHDVAKVQIYCAWAEALLGEASFSKPIMTASGEAAMMHADNAMGRHNGMA
                     MSR"
     gene            1797..2276
                     /locus_tag="VLD60_11950"
     CDS             1797..2276
                     /locus_tag="VLD60_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB45720.1"
                     /translation="MFRLLVAAAATMVMAEGAQAQLIEKTALTLDGAKKIAAVAEAKA
                     KAEGARVVIAVVDENGSLLLLERLDDTQVASVNVGIDKARTAAIYRRPSKVFEDQVKN
                     GRISALALHGAVALQGGVPILVNGKVIGAIGVSGETPAQDEDIAIAGAAVAASFATH"
     gene            2373..3287
                     /locus_tag="VLD60_11955"
     CDS             2373..3287
                     /locus_tag="VLD60_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB45721.1"
                     /translation="MKQTLALIAGIVVMGTAALLHAESVADKRGLTLEGAKKVIAATV
                     AEAKRVNAPGGSIAVVDEGGNLLALERLDQTFAASANIAIGKARTSALFKKPTKVFED
                     VIKSGRTSMITLGGDLQNFTPLQGGIPLEWEGKVVGAIGVSGAASAQQDEELATVGAK
                     SLDSMELAGMTNGHSPLPVTYIDSQKVTASFQKGAVLLGEDENMMHAARNYMVHASHR
                     DKAGLVEIHEQDTDIVYVLKGSAQLITGGTPVETKTIAPHEFRAPSVNGGEMRRLVPG
                     DVVIIPNGVPHWFKEVVAPFDYYVVKVR"
     gene            3454..5076
                     /locus_tag="VLD60_11960"
     CDS             3454..5076
                     /locus_tag="VLD60_11960"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB45722.1"
                     /translation="MEIRSVMVLAVMVMSGQPSFAQVTGDAPGVRPDAIIDLKTEEGM
                     ALVTGQWRYSNARVIDVDHHSPGADLAPSGPPNRTQDIDVHAGASDFDDSQWEQIKPA
                     QLEERRSSGRLCFNWYRTSLTIPEKIGSFDPTGSTVVFEISIDDYAEVWVDGKLPLVL
                     GQPGGQLIKGFNAPNRVVLTKTAKPGQKIHLAVFGINGPLSNPPGNFIWVRSATLDFY
                     KSHRIGPTQFVKTEIISTDPALDRIVTPGTRLEQLAGGFLFTEGPVWVTATATTSGYL
                     LFSDPNNNTIYRWSPEGQVSVFRTKSGYSGLNAGEYHQPGSNGLTLDSKGRLTINQHG
                     NRRVIRVEPRGNITVLADRYDGKRLNSPNDLVYRSDGALYFTDPPFGLPNVFADPRKE
                     LPYSGVYCVKDGLVKLVSTDLDAPNGLALSPDEKTLYVNNWNDKRKVILRYDVNQDCT
                     LANSRLFFDMTNAPGMDALDGLKVDQEGNVYSTGPGGLWILSPEGKQLGLIKGPQDPH
                     NMAWGDDDGKTLYITALTGLYRIRLNIAGVRP"
     gene            5073..6083
                     /locus_tag="VLD60_11965"
     CDS             5073..6083
                     /locus_tag="VLD60_11965"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB45723.1"
                     /translation="MMRTSSSLRIVLGLIVLLQAGAVWAETVPATITSLEPRFDQLVP
                     KDAKIEKIADGFTWVEGPVWHKQGGYLLFSDIPANSIYKWKPGEGVSLFLKDSGYSGA
                     TPFTGKEPGSNGLTFDAYGRLVLCRHGDRQIGRLETDGRISAVADHYNGYRINSPNDL
                     VFKSNGDLYFTDPPFGLPNAFDDPDKAPVQGVYRVSKDGTVTRLITDIKAPNGIAFSP
                     DETTLYVSDVDPKRAAWLAYDVKQDGTVTNGRLFFDGTRWRKEPFFGPDGFKVDQNGN
                     LFGARPGGLSVIAPDGTLLGTIDTGQPTSNVAWGEDGQTLFITGGSSVYRVRLATKGA
                     AY"
BASE COUNT         1439 a         1840 c         1832 g         1198 t
ORIGIN      
        1 gcgcaggaaa aacgaccgcg atgaatctga tccaccggtt ttacgatccg accgagggac
       61 gaatcaccat cgatggcctg gatctccgcc aggtcaccct ggaaagctgg tatcggcaga
      121 tcgcactcgt gccgcaagaa acgattctgt ttggcggtac cattctcgac aacatccgct
      181 acggtaaccg ggaagccagc gaggaaatgg tggaagccgc cagccatgcg gcccacgccc
      241 acgatttcat catgagcttc ccggaccagt atcacaccgt cgtgggagaa aaaggcatca
      301 acgtatcagg cgggcagcgg caacgcatcg ccatcgctcg tgcgattctc aagaatccgc
      361 ggattctctt gctggatgag gccacttcag cgctcgacag cgaatcggag cggctggtgc
      421 aggaggcgtt ggagcagttg atgaaaggcc gaaccacgtt cgtgatcgcc catcgtctca
      481 ccaccatcca gcgggccgat cgcattctcg tcctgaataa aggccgtctt gtggaaaccg
      541 gcacgcacag tgaactcatg gaccgtcaag ggctctatca ctatctctat actctccggc
      601 tgacggaact gccctcgtaa ctcccgcccg gcgcgcgtaa tctcctgcgc atctctgtaa
      661 gaccagcttg ccttgaccga ccagggatca gacaactgca tccggatatg cagctcatct
      721 ccgggaggcc acgatgacgg actgtgatgc gctagaactc gcgattctcg aacgggtggc
      781 gtcgggcgca ccgctgagct tcgaagatct ccagcagttc cttcgtcacc acagctggaa
      841 ccgtttgttt gccgccgtgg atcaactgag ccgcaagggc tcattggcga tcaggcgcat
      901 cgatcgttgc acctatcaca tttcacttgg gccgcagttc gctacgcagg caagcggcat
      961 ccaaacggac aatgcgtcca cttctgtcac cactcagtca taggtttagc tcgcccgtgc
     1021 cgccgaggat gcgacggcgg tacttgtagt tcctttcaca tcgattcatt ccttctctac
     1081 tcaccctttc actcacctgt aagaactacc ccgatcagga gacgaacggc cagaagagca
     1141 gaaacgcgcc acgctgaatc attcagcgac agcccttcac ccactctcaa ggaggtttgc
     1201 aatgcattcc acacgttcat tcatactcag cttgtgtttc atggtcgcag cagtcggatt
     1261 gttcaccgca gccaacagtt ttgcggccga tgggcacacg acgtcgaagt tcgaaggcgt
     1321 caaagccaat agcggcacgg cgacccacgc acggtcgggc aacaacgata cgttgacctg
     1381 gtccgatgat ttcaagattc cggaaacgcc ggcgccccac tggcaggtga tcgacaccaa
     1441 gggcaatgtg tatttgctca atcgtttgaa gattaagggc ggagtgctcg gcggggaaaa
     1501 agagaaccgc acgatcacca tcccgtcata catacatgat gtcgccaagg tgcagatcta
     1561 ctgcgcctgg gccgaggcgt tgcttggcga agcgtccttt tcgaaaccga tcatgacggc
     1621 gtccggagaa gccgccatga tgcacgccga caatgccatg ggcagacaca atggcatggc
     1681 gatgagccgg taatggaaag caatcagcgg tcagcagtca gctctcggct tcttcggagg
     1741 tctggggctg acagctgacg gctgatagct ggcagcttac tgaagggaga caacacatgt
     1801 ttcgattgtt ggttgctgca gcagccacga tggtaatggc tgagggggcg caggcccaac
     1861 tcatcgaaaa gacggcgctc acattggacg gcgcgaagaa gatcgctgcc gttgccgaag
     1921 ccaaagcaaa ggccgaaggg gcacgggtcg tgattgctgt cgtcgatgaa aacggcagtc
     1981 tattgctcct agaacggctg gatgacacgc aggtggccag tgtgaacgtc ggcatcgaca
     2041 aggcaagaac tgcagcgatc taccgccgcc ccagcaaagt attcgaagac caggtcaaga
     2101 acggccgcat ttccgctctg gccttacacg gagcggtggc tttacaaggc ggcgtgccga
     2161 ttctcgtcaa cggcaaggtc atcggagcga tcggcgtgag tggagaaacg cccgctcaag
     2221 atgaggacat tgccatcgcc ggagcagcgg tcgcggcatc attcgccaca cactgactgg
     2281 caacgttcag cgatcagctc tcagctcagg agaatgtgcc cgaagctgga agctgatggc
     2341 tgaatgttga tcgatcaaga agggagaaca ccatgaaaca gacactcgcg ctcatagcag
     2401 gaatcgtcgt gatggggacg gcggcgcttc tccatgcgga gtcagtggcc gacaaacgcg
     2461 gactgacgct cgagggagcg aagaaagtga ttgcagcaac tgtcgccgaa gccaagcgcg
     2521 tcaatgcgcc gggcggctcc attgccgtcg tcgacgaggg aggcaatctc ctggccttag
     2581 aacgattgga ccagaccttt gccgccagcg ccaacattgc gatcggcaaa gcgcgcacgt
     2641 ctgccctctt caagaaaccg accaaagttt tcgaagacgt gatcaagagc ggccgcacat
     2701 cgatgatcac gctcggaggc gatttacaga atttcacgcc gctccaaggc ggcatccccc
     2761 tcgaatggga aggcaaagtc gtgggcgcca tcggtgtgag cggcgcggca agtgcacagc
     2821 aggatgaaga acttgccacc gtcggtgcca aatcgctgga ttcgatggag ctggccggca
     2881 tgacgaacgg ccactcgccg ttgcctgtca cctacattga tagtcagaaa gtgaccgcct
     2941 catttcaaaa aggggccgtc ctcctgggtg aagacgagaa catgatgcac gcggcgcgca
     3001 actatatggt ccatgccagc catcgtgaca aagccggcct cgtggagatc catgagcagg
     3061 atacggacat cgtctatgtt ttgaaaggct cggctcagtt gatcaccggc ggcacgccgg
     3121 tagaaacgaa gaccattgcg ccgcacgaat tccgcgcgcc atccgtgaat ggcggagaga
     3181 tgcgcaggct ggtgccggga gatgtggtga tcattccgaa cggagtgccg cactggttca
     3241 aagaggtcgt ggcgccgttt gattattacg tggtgaaagt ccggtgaggg cttggggagg
     3301 ggtggccttc tcgctcgcgg aacgcgcaca caagattaat cgtatcgaca aatagagagc
     3361 ggtgctcgtt cgacgcgcgc ggcagaggcc aacccctccc cgcccatcgg aagagataag
     3421 cgcgagcaaa cggctcagag atggagagac aagatggaga ttcggagcgt gatggttctt
     3481 gccgtgatgg tgatgtccgg acaaccatcc tttgcgcagg tgactggcga tgcgccggga
     3541 gtgcgaccgg atgcgatcat cgacttgaag accgaagagg gtatggcgtt ggtcacgggc
     3601 cagtggcgat atagcaatgc cagggtgatc gacgttgacc accatagccc gggcgcagac
     3661 ctcgcgccat caggcccgcc gaatcgaaca caggatatcg acgtacatgc cggcgcttcc
     3721 gacttcgacg attcgcagtg ggagcagatc aagccggccc aactcgaaga acggcgttcg
     3781 agcggacggc tctgcttcaa ttggtatcgc acaagcctca cgatcccgga gaaaatcggc
     3841 tcatttgacc cgacgggatc gaccgtcgtc tttgaaatct ccatcgacga ttatgcggaa
     3901 gtctgggtcg acgggaaact cccgctcgtc ttggggcagc caggcggaca gctgatcaag
     3961 ggcttcaacg cgcccaatcg ggtcgtgctt acgaagaccg ccaagccagg ccagaagatt
     4021 catctcgctg tcttcggtat caacggtccg ttgtcgaatc cgccgggaaa ttttatctgg
     4081 gtccgatccg ccacgttgga cttctacaag agccatcgca tcggcccaac gcaatttgtg
     4141 aagaccgaga tcatctcaac cgatccggct ctcgatcgta tcgtgacacc gggcacccgg
     4201 ctcgaacagc tcgctggtgg cttcctcttc accgagggac cggtgtgggt cacggccacc
     4261 gccaccacat cgggatatct cctcttcagc gatcccaaca acaatacgat ctaccgttgg
     4321 tcacctgaag ggcaggtatc cgtcttccgc accaagagcg gctacagcgg tttgaatgcc
     4381 ggcgagtacc atcagcctgg ctcaaacggg ctcacgctcg acagcaaggg ccgcctcacg
     4441 attaaccaac acggcaaccg ccgtgtcatt cgcgtcgaac cccgtggcaa catcaccgtg
     4501 ctggcggatc gctatgacgg caagcgcctg aacagcccga acgatctcgt ctatcgatcc
     4561 gatggcgcgc tctacttcac cgatccaccg ttcgggttac cgaatgtctt cgccgatccg
     4621 cgcaaggaac tgccctacag cggcgtctac tgcgtgaagg atggcctggt caagctggtg
     4681 agcactgatc tcgatgcgcc gaacggtctg gcactctcgc cggacgagaa gacgctctat
     4741 gtgaacaact ggaatgacaa acgaaaagtg atcttgcgct atgacgtcaa tcaggattgc
     4801 acgctcgcga acagccggct cttctttgat atgacgaacg cgccgggtat ggacgcgctc
     4861 gatggactga aggtcgatca agaaggcaat gtctactcga ccgggccagg cgggctctgg
     4921 attctttcgc cggagggcaa gcaactcggc ctcatcaaag gcccccaaga tccgcacaac
     4981 atggcctggg gcgatgacga cggcaaaacg ctttacatta ccgcactgac cgggctctat
     5041 cgcatcagac tgaacatcgc aggggtgaga ccatgatgcg aacatcctca tcgcttcgga
     5101 tcgtccttgg gctgatcgtt ctcctgcagg cgggagcggt atgggcagag acggtccccg
     5161 ccaccatcac cagcctggag ccgcgcttcg atcagcttgt gccgaaggat gccaagatcg
     5221 agaagattgc cgacggtttt acctgggtcg aaggaccggt ctggcacaag cagggcggct
     5281 atctcctctt ctccgatatt ccggcgaaca gcatctacaa atggaagcca ggcgagggtg
     5341 tgagtctgtt cttaaaggac agcggctaca gcggggcaac acccttcacc gggaaggaac
     5401 ccggctcgaa cggactcaca tttgatgcct atggacggct cgtgctctgt cgtcatggcg
     5461 accggcaaat tggtcgattg gaaacagacg gcagaatcag cgctgtggca gaccactaca
     5521 acggctatag gatcaacagc ccgaacgatc tcgtcttcaa atccaatggt gatctgtact
     5581 tcaccgaccc tccgttcggg ctcccgaacg ccttcgatga tccggataag gcaccggtcc
     5641 agggtgttta tcgcgtgtcg aaagacggaa cagtcacgcg cttgattacc gacatcaagg
     5701 caccgaacgg aatcgccttc tcaccggatg aaacaacgct ttatgtttcc gacgtcgatc
     5761 cgaaacgggc cgcctggctc gcctacgatg tgaagcagga cggcacggtg acgaacggcc
     5821 gcctgttctt cgatggaacc cgctggcgca aggagccgtt cttcgggcct gatggattca
     5881 aagtcgatca gaatggaaat ctctttggag cccgaccggg aggactcagc gtgattgctc
     5941 ccgacggcac actcctcggc acgatcgaca ccggccaacc gacgtcgaat gtggcctggg
     6001 gcgaagatgg acagactctt ttcatcacgg gaggctcttc ggtctaccgc gtgcgattgg
     6061 ccacgaaggg tgccgcctat taggtccgtg cacgaacatc gattgccctc ctcttgcctc
     6121 cgtttcatcc cctggctcat acttggcgta ggagtctatg gccgaccaac cccataacca
     6181 tccacagcca ttgagcggtc tggaacccga cgccgccctc ctcgccattc tggaaggaac
     6241 agctgcggaa acgggagagg gatttttcgc cgcgttggtg gagaacctgg cgaaagcgct
     6301 cggtacaca
//
LOCUS       DATIFR010000208         8072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1100264, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000208 DATIFR010000000
VERSION     DATIFR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100264"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VLD60_11970"
     CDS             complement(<1..565)
                     /locus_tag="VLD60_11970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSB45724.1"
                     /translation="MSEFLDTTCSPEALLSVAVEAARAAGTILLSYAKSGFRITHKSP
                     INLVTDADHAAEQCVIDLIRAQFPAHHILAEERGSDVQPDSRYHWVIDPLDGTTNFAH
                     GFPVFAVSIGVELDGQGLIGVVYDPTRDELFTARAGSGARLNGQPIAVSITDQLDRAL
                     LATGFAYDIRDTPHNNLDHFARFALKAQ"
     gene            780..1286
                     /locus_tag="VLD60_11975"
     CDS             780..1286
                     /locus_tag="VLD60_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB45725.1"
                     /translation="MEELTDILVGLEQRINAYKNRLQELEKKRRRLDDEIATIKKYLE
                     LAETLYRVEADKAKLASLSTQLLSDEKGTRTLPVDVTDQSREILLGRSKYAGKSVPEA
                     AYQMLREANRPMHAKELVQRLVEGGLQIKGKTPLTSVATSLKRDKRFRKVGPNTFEAL
                     EEMLIHAV"
     gene            complement(1504..2439)
                     /gene="lpxC"
                     /locus_tag="VLD60_11980"
     CDS             complement(1504..2439)
                     /gene="lpxC"
                     /locus_tag="VLD60_11980"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSB45726.1"
                     /translation="MRLQQTLANPVSCIGVGLHSGEPVTMTLRPAPPDTGVVFVNRNG
                     HAGASLAASVDHLVPTELCTAISGNGFQVKTIEHVLAALAGLNVDNVYVDVDAGEAPV
                     MDGSAGPFVRLIQSAGIISQKKLQPYLKMTRPLEVVDGSRRIRIEPSATPQITYSIQY
                     DHPLIHMQTYSYEHSARTFESEIAGARTFGFLQEVEALWARGLGRGGNLDNTVVLSKD
                     GVLNESGLRFTNEFVRHKVLDLIGDFALLGLPFIGHLIADRSGHALHTRLVKQILEHP
                     ECWVLLNTNHSTAQSQSLSSHAAPRSSSAVALQAS"
     gene            complement(3257..4975)
                     /locus_tag="VLD60_11985"
     CDS             complement(3257..4975)
                     /locus_tag="VLD60_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HSB45727.1"
                     /translation="MSPSIDVAEHIAQARQMDGVSSTLSWSSFADFFKSRVYDRTLVN
                     QPFLTYYDDDRHLHYTYSYSEFGTVVQRAAAYLHDEIGLRRGDRLATILFNHDITVVL
                     YFAAWISGITVVPINVEEPTDKKRFILEHSETSAVCCWHSHVEEMCDIQRDLPALRHV
                     IALNDQGFLEGAEPGSRDKGAGPPAASRLAPHTSSLNDEALIVYTSGTTGPPKGVVLT
                     VENLLIDADAIADCHHFGVNDRLMCVLPIHHVNGIVVTLITPFYFKGSVVLNRKFKSA
                     GFWRRLHEEQVTCISVVPTLLEFLLDADEDRSPYRLDRFGGFICGAGPLHKDTAARFE
                     DRFGFPIRHGYGLSETTCYSCFLPNDPLPEEHRHWIRDYEFPSIGVPLRHNRMTILNE
                     NGQSLPELSRGEICIRGRTVCAGYFKRDDANDAAFKWGWFRSGDEGFYVRDNKGRPFF
                     FISGRLKELIIRGGVNISPLEIDEALKSHVHIQFAMAVPFEHRYYGEEIAAYVVPRVG
                     ASPPTEAELLEHCRRLLPFSKCPKVILFGQEVPYTSTGKPKRLELKTRLASPLSAYRD
                     HQFKER"
     gene            complement(4950..6158)
                     /locus_tag="VLD60_11990"
     CDS             complement(4950..6158)
                     /locus_tag="VLD60_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSB45728.1"
                     /translation="MQSMNNGIVNFTQRTMTMPTVDTTKLRTYSPGLEGVLAGETALC
                     HVDEGEGGLRYRGYAVSDLADKATFEEVAHLLLFGKLPTQKELKDFSAQLVAHSVLPD
                     PVAALLGVVPPAAHPMDVLRTSTSLLGMTDPDASDNSHDVNVRKSVRLLAQIPLIIAT
                     AHRLANGKPRVEPQPDLSFAENLLFLLTDQRGDERARAMARVLDVSLTLYAEHEFNAS
                     TFAARVTASTMTDLHSAITSAIGTLKGPLHGGANEAVAEMFLEIESRERAERWVREAL
                     ANKHRIMGFGHRVLKKGDARSAIIQQHAESLSRICGDRRWYEIATTIDRVIQEEKGLY
                     PNLDFYTAVAYLLMGIPRDLYTPVFVCSRITGWCAHVIEQQDHNRLIRPRALYTGPAS
                     QAYVPLDRRR"
     gene            complement(6094..7005)
                     /gene="prpB"
                     /locus_tag="VLD60_11995"
     CDS             complement(6094..7005)
                     /gene="prpB"
                     /locus_tag="VLD60_11995"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HSB45729.1"
                     /translation="MYPQPTSKARRLRERLDKQIVVLPGAFNALTAMQIERAGFHALY
                     VSGAGLSAARGLPDIGLLSLTEVVSDAAAITKAVTIPAIVDADTGFGPPLVVMRAVKE
                     FEQAGLAGMQIEDQELPKKCGHLPGKRLVSTSDMVNKICAASDARQDPEFFVIARTDA
                     RAVEGLDAAVRRARAYIDAGADAIFPEALESADEFRAFARQLAREGGKAPLVANMTEF
                     GKTPYLTVSEFEDLGYRLVLFPVTALRLATKAIEQMLIELRSHGSQRELLDRMHTRQQ
                     LYELLRYAEYEQRDRQFHPADDDHANG"
     gene            complement(7007..>8072)
                     /locus_tag="VLD60_12000"
     CDS             complement(7007..>8072)
                     /locus_tag="VLD60_12000"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSB45730.1"
                     /translation="GEASRASGKRVIQAIALAYEVQCRLCDAAALRPRGWDHVTYGPF
                     SSALAAAKTMKLSEAQTVQAVNLAGVANVALRQTRVGDLSMWKACAFSNAARNGVFAA
                     MLAQQGMTGPSPIFEGEKGFMKLVSGPLELPPFGGEKGSSNTGSFKILDSYVKHYPVE
                     YHAQTAVEAALSIREELLKTKGAEALAGIAEIDIASYDVAIEIIGHDPEKWRPRTRET
                     ADHSFPYCVAVALLDGKVTIRSFEAKRLADPVLYEMTQKVRVIPQPEFVGRYPQALPT
                     RITVRTKTGSVYVHQVDYPLGHPRNRMSDSEIEAKFRSLAAGKLDRARTNKVLDIVWK
                     LDQLKEINALMPLLKMHRRN"
BASE COUNT         1822 a         2211 c         2338 g         1701 t
ORIGIN      
        1 cttgagcttt cagcgcgaat ctcgcaaagt gatccagatt attgtgcggc gtgtcacgga
       61 tgtcgtacgc gaaaccggtc gccaggagtg cgcgatcgag ctgatccgtg atcgacaccg
      121 caatcggctg tccgttgaga cgcgctccgc tacctgcgcg cgccgtgaag agctcgtcac
      181 gggtcgggtc atacaccaca ccgatcaggc cctggccgtc caattccacg ccgatggaca
      241 cagcaaagac agggaatccg tgggcaaagt tggttgtgcc gtcgagagga tcgataaccc
      301 agtggtatcg ggaatcaggt tgcacatcag agcctcgttc ttctgccagg atgtgatgtg
      361 cgggaaactg ggcgcgaatg agatcgatga cgcattgttc ggcggcatga tcggcgtcgg
      421 taacgaggtt gataggactt ttgtgcgtga tgcgaaatcc ggacttcgcg tagctcagca
      481 aaatagtgcc cgccgctcgg gcggcttcaa cggccaccga caacagggct tcaggcgagc
      541 atgttgtgtc gagaaactcg gacataggcg ggcatcatag catgggatcg gagtgagacc
      601 aggctgcttg catcagtttc tatcagtagc aggaaagatc gaaatcaccg ctacaaatag
      661 acggccatgg tcatccgtca taggttcgat catcacgcaa ggcattacaa acacattctc
      721 tattgacttg cttcacaagc aaggatataa agcaagcatg cttgttaagg agcccgcctt
      781 tggaagaact caccgatatc ttggtcggtc ttgaacagcg gatcaacgcc tacaagaacc
      841 gactgcaaga gctggaaaaa aaacgtcgcc gcctggacga cgaaatcgcg accatcaaga
      901 aatacttgga attggcagaa acgctgtacc gcgtggaagc cgacaaggcc aaactggcaa
      961 gtctctcgac acaactgttg agcgacgaga aggggacgcg gactcttccc gtcgacgtga
     1021 cggatcaatc acgggaaatt ctcttaggac ggagcaaata tgccgggaag agcgtaccgg
     1081 aagcggccta tcagatgctg cgcgaggcga accgccccat gcatgcgaaa gaacttgtcc
     1141 aacggctggt tgagggcggc ctccagatca aggggaagac gccgctcacg tccgtggcga
     1201 catcgttgaa acgcgacaag cggttcagaa aagtcggccc gaacacgttc gaggcgctcg
     1261 aggaaatgct gatccacgcg gtgtaaccac gaacgactca caattggaat gatgcgaagg
     1321 ggggtgagag tcttggcgac gaagaaagca gcgaagaaga aaaagaagta agtcccgcct
     1381 cgattctagc gtcgggggcg aggccactcg ctcccgacgt tattacagcc agcgacctct
     1441 ccggtagaga caacccgctc cactacatca ccgagcgcca gtccgcggta atccgatgaa
     1501 aagttaggaa gcttgcagcg ctacagccga ggacgatctc ggtgcggcgt gagaggaaag
     1561 cgattgagat tgggcggtcg aatgattggt gttgaggagg acccaacatt ccggatgttc
     1621 gagaatctgc ttcaccaagc gtgtatgcaa cgcatgtccc gatcgatcgg caatcaagtg
     1681 tccgataaag ggaagtccga gaagcgcgaa gtcgccgatc aggtccaaga ccttgtgacg
     1741 gacgaactcg ttcgtaaagc ggagtcctga ttcattaagg acgccgtcct tggagaggac
     1801 gaccgtatta tcgagattgc caccgcgacc taatccgcga gcccagaggg cttccacttc
     1861 ctgcaagaag ccgaaggtcc gcgcgccggc gatctcggat tcgaacgtcc gcgccgaatg
     1921 ctcataggag taggtctgca tgtgaatcag cggatgatcg tactgaatcg agtatgtgat
     1981 ttgcggtgtg gctgacggtt caattctgat tcggcgagac ccatcgacca cttcaagagg
     2041 tctggtcatc ttgagatacg gctgaagttt cttctgtgaa ataatgccgg ccgattgaat
     2101 tagccgaaca aaaggcccgg cactaccgtc catcaccggc gcttcgccgg catcgacgtc
     2161 cacgtacacg ttatcgacgt tcaggccggc cagcgccgcc agaacgtgct cgatggtctt
     2221 gacttgaaaa ccgtttccgc tgatggccgt gcagagctca gtggggacca aatgatccac
     2281 agaggcagcg agagaggctc ccgcatgccc gttccgatta acgaacacca cccccgtatc
     2341 cggaggagct ggtctgagcg tcatggtcac aggctctccc gaatggaggc cgacccctat
     2401 gcaactgacc ggattcgcca aggtttgctg cagtctcata cagatcctcc gaaaagcact
     2461 gctgactaca gcaactctga tgccatcttg cgtcagatcg gaacagatac ataagtcgtg
     2521 gaaataatta atgaaaagtg atcgtgcgct caattggttc catgtagcag aaatgtgaca
     2581 tctgtgactt tttcaaaaga atcgtggggt tatattgccc atgaagatgg aggagcgaat
     2641 gaggggcgca tttgtgattg tcacattaaa gttgtcgacg atcgccaaaa ccccttctct
     2701 agaaagcagc aacttactag gaggatccaa aggggtgcaa gggggaaagt tgtagactaa
     2761 cgcttttgtc aaacatctta tactgcgttc agcatgacat taaagtagta gacattttct
     2821 gagttgctcc cccctgctgg ttcgccacgc gtgtgatcat tagcttcttg atcgctcaca
     2881 cagcaatgca gattcatcag accgccgata atgttcaatt catatgattt ccagtgagag
     2941 gggaaaggag atgcctaaca tactgtgtaa ccatatccct ttgagaacag cgggcgcttg
     3001 aacgaagact acatctcgcc ggcaaatgct tcgtgaagga gaaattagca agcccattga
     3061 tcgatacctg ggtaaaatgg gtcaagacta tactcaggcg gatcgttcac cgcgtgtatt
     3121 ataatgacaa aggagtgtcg ctcccaccct cacccggcga tcgtttcgcg ccgggtatcg
     3181 caacacattc catcactgtc cagaacatcg atggctcgga acggataagg gggtgccggg
     3241 cccattacaa cctcttctac ctttccttga attgatggtc ccggtaggca gacagggggg
     3301 acgcgaggcg ggttttcagc tccaaacgtt tgggtttgcc ggtcgaggtg tagggaactt
     3361 cttgcccgaa gagaatgact ttgggacatt tagagaacgg gagtaatcga cggcagtgtt
     3421 caagcagttc cgcttctgtg ggcggagagg cacccactcg cgggacgacg taggccgcga
     3481 tttcttcacc gtagtagcgg tgttcaaacg gaacagccat ggcgaactgg atatggacat
     3541 gactcttcaa cgcttcgtcg atctccaacg gtgaaatatt caccccacct cgaatgatca
     3601 attccttcag gcggcctgaa atgaagaaaa atggccgccc tttgttgtct cgcacgtaga
     3661 atccctcatc tccggaacgg aaccagcccc atttgaaggc ggcatcatta gcatcgtccc
     3721 gtttgaaata gcccgcacag acagtcctgc cacggatgca aatctctcca cgcgacagtt
     3781 ccggcagtga ctgtccattc tcgttgagga tggtcatcct gttatgtcgt aacggcacac
     3841 cgatggatgg gaattcgtag tctctgatcc agtgacgatg ctcttcaggc aatggatcgt
     3901 tcggcaggaa gcaggaatag cacgtcgttt cagacaagcc gtagccatgg cggataggaa
     3961 agccgaatcg gtcttcaaaa cgggccgctg tgtctttgtg gagaggaccc gcgccgcaga
     4021 tgaatcctcc gaaacggtcg agtcggtacg gtgaacgatc ttcatccgca tcgaggagaa
     4081 attcgagcaa ggtgggaacc acgctaatac aggtcacctg ctcctcatgt aaccgtcgcc
     4141 agaatccggc gctcttgaat ttgcgattca acacgacgct gcctttgaaa tagaaggggg
     4201 tgatgagtgt gaccacaatt ccgttcacat ggtggatcgg gagcacgcac atcagtcgat
     4261 cgttcactcc gaagtggtgg cagtcggcaa tcgcatcggc gtcgatcaag agattttcca
     4321 ccgttaagac cacacccttg ggcggtcctg tcgtcccgga ggtatagacg atgagcgctt
     4381 catcgttcag gctagaagta tgtggcgcaa ggcgagaggc cgcagggggc cccgccccct
     4441 tgtctctaga cccaggttcc gcaccttcca agaagccttg gtcgttcagc gcaattacat
     4501 gccgtaaggc gggtaggtct cgctgaatat cgcacatttc ttcaacgtga ctgtgccagc
     4561 aacagacggc cgaggtttcc gagtgctcca gaatgaagcg cttcttgtct gtcggctctt
     4621 caacgttgat cggcacgacc gtgatgcccg atatccaggc agcaaaatac agcaccacgg
     4681 tgatgtcatg gttgaacaag atcgtggcga ggcggtcccc gcgtcgaagc ccgatctcat
     4741 catggagata ggcggccgcc cgttgaacca ccgtaccgaa ctcggaatag ctgtaggtat
     4801 agtgaaggtg tcggtcatcg tcgtagtagg tcagaaacgg ctgattgacc agggtccgat
     4861 cgtagacgcg cgacttgaaa aagtcggcga acgaactcca cgacagtgtg gatgagacac
     4921 catccatctg ccgtgcctgc gcgatatgct cagcgacgtc gatcgagggg gacataggcc
     4981 tgcgatgctg gtcccgtata aagcgcccgt ggacgaatga gcctgttatg gtcctgctgt
     5041 tcgatcacgt gcgcgcacca gccggtgatg cgcgagcaga cgaataccgg agtgtagaga
     5101 tcacgcggaa tccccatcag aagatacgcc accgccgtat agaagtcgag gttggggtaa
     5161 agcccctttt cctcttggat cacgcggtca atggtagtcg cgatctcgta ccaacggcgg
     5221 tcgccacaga tccggctcaa tgattcggcg tgctgttgaa tgatagccga gcgagcgtcg
     5281 cctttcttga gcacgcgatg gccgaatccc atgatccgat gtttgttcgc caacgcctcc
     5341 cgtacccatc gttctgccct ctcgcggctc tcgatttcga gaaacatctc ggcgacggcc
     5401 tcattcgctc caccatggag cggtcccttc agcgtgccga tggctgacgt gatagctgag
     5461 tgcagatccg tcatcgtcga cgcggtcacg cgggccgcga aggtggaggc attgaattca
     5521 tgctcggcat agagtgtaag cgacacgtcg agcacacggg ccatcgctcg agccctctca
     5581 tcacctctct ggtcggtgag gaggaacaat agattttcgg cgaagctcag gtctggttgc
     5641 ggctccacgc ggggtttccc gttcgcgagc cggtgggcag tggcgataat gagaggaatt
     5701 tgcgccagca gccgtactga tttgcggacg ttcacatcat gggaattgtc ggacgcgtcc
     5761 ggatcggtca tgcccaacag ggaagtgctg gtcctgagaa cgtccatggg atgtgcagcc
     5821 ggaggaacca cgccaaggag cgctgcgaca ggatcgggaa ggacggagtg tgcgacgagc
     5881 tgggccgaaa agtccttcag ctctttctgg gtcggcagct tgccaaagag cagcagatgg
     5941 gccacttcct cgaaagtggc cttgtccgcc aaatcgctga ccgcataccc gcggtatctc
     6001 agcccgcctt cgccttcgtc cacatggcag agggcagtct ccccggcgag gactccttcg
     6061 agccccgggc tgtaggtccg aagttttgtt gtatcaaccg ttggcatggt catcgtccgc
     6121 tgggtgaaat tgacgatccc gttgttcata ctctgcatac cgcagcagct catagagttg
     6181 ctgtctggta tgcatccggt ccagcagttc gcgttgggaa ccatggcttc tcagctcgat
     6241 tagcatctgc tcgatagcct tcgtcgccag ccggagagcc gtgacgggga acaggaccag
     6301 acgatagccg aggtcttcaa actcgctgac agtcaggtag ggtgtcttgc cgaactcggt
     6361 catgttggcg accagcggcg ctttgccgcc ttccctcgcg agttgtctgg caaaggcgcg
     6421 gaattcgtcg gcggactcga gcgcctccgg gaaaattgcg tctgcgccgg catcgatgta
     6481 ggcccgtgcc cgtcgcactg ctgcatccaa tccctcgacg gcgcgtgcgt ccgttcgggc
     6541 gatgacgaag aactccggat cctgtcttgc atcgcttgct gcacagatct tgttcaccat
     6601 gtcgcttgtg gaaaccagcc gcttccccgg tagatggccg catttcttgg gcaactcttg
     6661 gtcttcgatc tgcattcctg ccagaccggc ctgttcgaat tccttcacag cccgcatcac
     6721 gacgaggggg gggccgaagc ccgtgtccgc atccacgatc gccggaatag ttacagcctt
     6781 ggtgatcgcg gccgcatcgg ataccacctc cgtgagggac agcaagccga tatccggcaa
     6841 tcctcgcgca gccgacagac cggcgcccga tacgtacagg gcatggaacc cggcacgctc
     6901 gatctgcatg gccgtcaagg cattgaaggc tccgggcaga acgacgatct gtttatcaag
     6961 tcgctcgcgc agccgccgag ctttggatgt gggttgagga tacatgttaa ttccttctgt
     7021 gcatcttcaa taacggcatc aacgcgttga tctctttgag ctggtcgagc ttccagacaa
     7081 tgtcgagcac cttgttcgtg cgtgctcgat ctaattttcc tgccgccaac gagcggaact
     7141 tcgcttcgat ctcactatcg gacatccgat tcctgggatg gccgaggggg tagtccacct
     7201 ggtggacata gactgaccct gtcttggtcc gaacggtgat gcgcgttggc agtgcctgtg
     7261 gatagcgtcc gacgaactca ggctgaggaa tgacgcgcac tttttgcgtc atctcataca
     7321 gaaccggatc cgcgaggcgt ttggcttcga acgagcgaat cgtaaccttg ccatccagca
     7381 aggccacggc cacgcagtag gggaagctat gatcggcggt ttctctcgtg cgggggcgcc
     7441 acttttcagg atcatgtccg atgatttcga tcgcgacatc gtagctggcg atgtcgatct
     7501 cggcgatgcc ggcaagcgcc tcggctcctt ttgttttgag caactcctct cggatcgaca
     7561 gagcggcctc caccgccgtc tgcgcgtggt attccaccgg gtaatgtttg acataggaat
     7621 ccagaatctt gaatgaaccc gtgttggaag accctttctc ccctccgaag ggcggaagtt
     7681 caagcggtcc tgagaccagc ttcatgaaac ccttctcacc ttcaaaaatc ggagaggggc
     7741 ctgtcatccc ttgctgagcc agcatggctg cgaacacacc gttccgcgcc gcgttagaga
     7801 aggcgcaggc tttccacatc gagagatcgc cgacccgtgt ctggcgcagg gcgacattcg
     7861 cgactccagc gaggttgacc gcttggacgg tttgggcttc tgagagtttc atggtcttgg
     7921 cggccgcgag agcggaggag aacgggccgt acgttacatg gtcccagccg cgcggccgca
     7981 acgccgccgc gtcgcagagg cggcactgga cttcataggc gagcgcgatg gcctgaatca
     8041 cgcgcttgcc ggatgcgcga gaggcctccc cg
//
LOCUS       DATIFR010000209        27829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2440176, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000209 DATIFR010000000
VERSION     DATIFR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440176"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..906
                     /locus_tag="VLD60_12005"
     CDS             49..906
                     /locus_tag="VLD60_12005"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSB45731.1"
                     /translation="MSSDWIDVQIYSAVDAGEVLGLIDDPAVQGAWQDGGTIHLYWPS
                     RNWSLDHLVRLRQILRDLDITGQPVPEILVQSIPNEDWNRQWAQSVKPLWIANRIVIR
                     PSWETVSLRPEQIEIVLDPKQAFGTGHHATTRMLVEWLETVIQGGETVLDVGTGSGLL
                     AMVALRLGAARALGIDNDQVAIDCAREYARANQFGAELTLECGTLRDGMPFDLVLANL
                     DRQTLLLLADQLAARTGGRLLLSSVLTDQRRDIVDAFAKVHLYPGQQRERDGWLAMEF
                     IPAQSCEGA"
     gene            906..1400
                     /locus_tag="VLD60_12010"
     CDS             906..1400
                     /locus_tag="VLD60_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSB45732.1"
                     /translation="MTLHSGRPPYPHVHQINISDGGVPKLPVLEAKVTEQGVAGDRQR
                     NLKVHGGPDRAVCLFSLELIERLQDEGHSIDVGSSGENLTLAGLDWEKLGPGVQLTIG
                     PEIRVEIASYCAPCEWNARWFRDGEYSRISQKKNLGWSRLYARVLQGGVVRPGDEVVI
                     NKSD"
     gene            1486..2145
                     /locus_tag="VLD60_12015"
     CDS             1486..2145
                     /locus_tag="VLD60_12015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45733.1"
                     /translation="MDRVLEPELMEDEQQVLAYAKADFEEENQGFVDRFREYFPDFSE
                     GHVFDLGCGPGDIPIRIAKVLPACLVTGIDASAPMIRLAEQAVAQAGLSGRVTFRCER
                     FQDIAGASRADAVLSNSLLHHVPNPLQFWNRLRQLVKPGSPVLVMDLLRPETPEEAQA
                     IVDRYAAGEPEILRRDFYNSLLAAFTEDEVTTQLARMNLTRLLIDVPDDRHWVVGGLI
                     H"
     gene            2142..2960
                     /locus_tag="VLD60_12020"
     CDS             2142..2960
                     /locus_tag="VLD60_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSB45734.1"
                     /translation="MNVAAYRALSALCLLLLFGLHVESSFAQLDRLRKSKPLDVQAPG
                     ETAMADIPEGFFVMGFDGTQALEDERPSHRVWLDTFAMDVHEVTTGQYAAFLAATGRT
                     VPWQWETVDLSQHRERPVIGVDWHDAQAYCQWKGKRLPTEAEWEKAARGTDGRLYPWG
                     NQVPTKEVANFALGARFSYSQVLLPVRSYEQGKSPYGLYQMAGNVYEWVQDWYATNYY
                     DTSPDRNPQGPGQGQFKVMRGGSWSDLPKYLLTYGRFKLPPETRNSYTGFRCAK"
     gene            complement(3048..3497)
                     /locus_tag="VLD60_12025"
     CDS             complement(3048..3497)
                     /locus_tag="VLD60_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45735.1"
                     /translation="MITENTQQILTVLYPLYKAEVYKRREQMMRLTAVGSLGLIAMTF
                     SLVLSPLKSRLSPPESFLLALAAMVWCGLFCGLILQQQDRHRLAKQVLIQLEQALGFY
                     EEGLFVEHQALYPGKWKTAWIGDRTGILYLSSLCTLTGIFLLALALT"
     gene            complement(3510..4289)
                     /locus_tag="VLD60_12030"
     CDS             complement(3510..4289)
                     /locus_tag="VLD60_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB45736.1"
                     /translation="MEEALTFHDDRGHRVAAVLSRPEQHTDRIVVLCHGFPSNKNSTT
                     NKTLTRLLNARGIATYRFDFFGQGESEGHFEDITTTLAVGQANAAINNVLSMGYNAIG
                     LAGSSFGGLVAILTAAQRQDIACLALKCPVVDFAEELRLEFGPDEMARWQATDTIQNI
                     MGGSERVRLKYRLYEDCLHLAAYGPATGITTPTLIVQGQKDELVPLHQSQKLFDLLRG
                     PKRIDLLPEADHQFTRAEDFHAMTTSITEWLAMHLIPSRTS"
     gene            complement(4324..5607)
                     /locus_tag="VLD60_12035"
     CDS             complement(4324..5607)
                     /locus_tag="VLD60_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45737.1"
                     /translation="MVSVRCTPLTFLLTGFTWLLLSSLIGLAILVGLIRGTPLPSWLR
                     LVHVHGALVGGVAQMIMGAMLAFIPPLLMTGQKRSDSHPILLAVVNGGALGMVTGFGF
                     QNFAIVAGGGLLVLGAFLSIASDAWRQTRQSLNSPPLNLLFYGLALIALFGGLAVGIG
                     MASRFIDMSWYGHARLVHIHLNLLGFVTLVIVGTMHNLLPTILQNKLYSPRLARAVFF
                     LLPTGMAVLIAGFALSSLKTELAAGILLVAGTGLYAFNLFASWKTSNQAGNAASDHLL
                     VATFFLVLAMVMGLLVGVNSLWDVPVFPFGTLHVVAYTHMALVGFVLQTIFGALSHLV
                     PITLAVSRVPNHKKREPYLATLTTIINRWRIVHIGGLSLGTLGLALVASLTWKMPLGA
                     PAIQAATWITFGLLLISLAFFAAKLAWALGTRPPQ"
     gene            complement(5614..6414)
                     /gene="thiD"
                     /locus_tag="VLD60_12040"
     CDS             complement(5614..6414)
                     /gene="thiD"
                     /locus_tag="VLD60_12040"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HSB45738.1"
                     /translation="MSTTLKQVLTIAGSDSGGGAGIQADIKAMSANGVFAMSVITAIT
                     AQNTEEVTDIFELPTSIVAAQIDAVFDDFEVAAVKTGMLSSTAVVELVAKMLKPQNVA
                     NLVVDPVMISKRGHPLLKPEAIEALKKQLFPLALLVTPNIHEAQLLSGIEIKALADAR
                     RAAKVIHGFGCKHVLIKGGHLLAERATDLLYDGRFFHVFKGEFIETPHTHGTGCTFAS
                     AIAAHLARGKPLNDAVQTAKAYLTEAIRHSLAIGHGTGPTNHFYFLGG"
     gene            complement(6493..6975)
                     /locus_tag="VLD60_12045"
     CDS             complement(6493..6975)
                     /locus_tag="VLD60_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45739.1"
                     /translation="MTTDQSTHRLTLFTMLVYWGCILLGLGIPWLATMGVDVLKHNQS
                     LADAAHHLDLHLFAPGYNIFLVGLLNAVPFVLFAIFVLFHMGLAPALDRKLCGRRAAG
                     VLVTAIGMIGLSAWTHVMTLWFPDAQGALAYLFLPFVLLGLMPIAYALGRGLGSLIFR
                     "
     gene            complement(6972..7958)
                     /locus_tag="VLD60_12050"
     CDS             complement(6972..7958)
                     /locus_tag="VLD60_12050"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB45740.1"
                     /translation="MTKRQIWLNLGGLGVLMALLGLVYWQHTGIRAVPGLIEDLQSQD
                     RQAQMLAAEGLKHIGLPAQAAVPALLELATSAGRSSLHSEAAGALPTIDLSAARRVMV
                     HYLPKLQDPDSQIRRDAASVLGALGPVARPAVQGLIGILNDSDTVVRERTVRALGAIV
                     LPTDRVMGGLLQALRDPEWTVRYAAVMQFSFSGFSSPESLAALRDLTKDSNQPVARLA
                     QSAVTAAERPTSIPVQLLMLDQPVDRTYPLLQLAKLGPRAAEAVPKLTSLLMSELPLE
                     RYLTASTLESIGPAAKEAVPALQQALHDADPVVREAVAAALESITRTASTTP"
     gene            8298..8939
                     /locus_tag="VLD60_12055"
     CDS             8298..8939
                     /locus_tag="VLD60_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45741.1"
                     /translation="MRTSAIWIGWIAGLTLVAGGLLLMFGCSTASAPPANTEAVRQHA
                     DKAFHDSKAQVQPPKQGDAQSPPAVSGTDDPATLASPSVQKGVQIPLAEIGPSDGETI
                     RATGYGPLAKGLTLCQHSADLAARVELSKLVRVQVTEKSVDRVRERTGKNFEQDIEVV
                     REGFVNEVLSDVRIVDRKVDKEAGTCSSTAVIPKRNLFPTSGNQTLEGRPISK"
     gene            9032..10033
                     /locus_tag="VLD60_12060"
     CDS             9032..10033
                     /locus_tag="VLD60_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSB45742.1"
                     /translation="MTPRIVLLVMATLLGVWPSIGVAGDLTPREIYERTSPAVVMIIG
                     YSNSGQRGSGGTGSIIQEDGLILTNAHVVIEEQTGRPYPRLTIYLKPDHVTGDPKVDL
                     SRGGKARVVAFSQTLDLALLRLEGMPGPFPALGLDDSDKTEIGDRVVAIGHPEQGGLW
                     TLTTGTISAEFENFNATKGKSVFQTETGLNRGNSGGPLLDTHGRMIGVNTAIVRVAAD
                     GLPITSISFSLKSNVAKRWLGEQGVSAQEALGPSDTPGLDKSASRSTSRPSGQTIPPT
                     NPPAAPTIVAPSSPTEAAAQVPSPRPYNLDRVISERSRAEADLEDMIKEMRGKLSGR"
     gene            complement(10185..10688)
                     /locus_tag="VLD60_12065"
     CDS             complement(10185..10688)
                     /locus_tag="VLD60_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45743.1"
                     /translation="MRRSILMLLGLTLCWPIVGFAFTADKRDTLRGLREVSVLVEYLP
                     DDVEREGLSREHLKHDVEARLGLAGLRVLTISEIANSPGAPYLYVAVYPITAPSVNFN
                     AYAVGLTLKQLVHLSRDPTTEFFATTWEGSAPPSSLSAPRVPDIRARILDAVGRFLID
                     YQAMNLK"
     gene            complement(11110..11778)
                     /gene="tenA"
                     /locus_tag="VLD60_12070"
     CDS             complement(11110..11778)
                     /gene="tenA"
                     /locus_tag="VLD60_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HSB45744.1"
                     /translation="MLFSDRLRQLATPIWDAQLNHPFVVALGNGTLPEQRFKYYILQD
                     ARFLGDLSRVFAAGAMRAPDSETALRFAKLAEETITVERSLHEGYGSRWNLSPKEMSS
                     VPMAPTNYAYTRHMLHVAASGSAAEITVVALPCAWIYCVVGQHLLKNGPPAKKHPYRD
                     WLMLYASPEFAEVQQWMRKKVDQWAKTAGKEEKRRMEEAFITSSRYEWMFWEMAWNEE
                     KWPV"
     gene            complement(11809..13212)
                     /gene="sthA"
                     /locus_tag="VLD60_12075"
     CDS             complement(11809..13212)
                     /gene="sthA"
                     /locus_tag="VLD60_12075"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HSB45745.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLNKTVGIIERKEVVGGVCIN
                     TGTIPSKSLREAVLYLSGFRQRNLYGAGYRVKDTITIEDLAFRANHVIKNEIAIVQNQ
                     MTRNRVDMIFGTARFVDPHCLQIQQAGGSIEHTADFIVIAVGTEPARPAEIPFDGESI
                     IDTDGLLTLKRIPDSIMIVGGGVIGTEYASILAALGVPVTLIDKRPRLLEFVDAEIIA
                     ALQRQMKDIGVTLYHEEEVLAIKKDTDNRIDVCLQQAKPIQATTLMYAIGRVGATTGL
                     NLEAVGLTPDRRGRLAVNAHFQTAVPHIYAVGDVIGFPALASTSMQQGRHASCHAFGY
                     PDRTDTDLLPYGIYAIPEISMVGKNEEELTQAEIPYGVGIARYKEIARGQLIGDESGM
                     LKLLFNRHTHELLGVHAIGEGATELIHIGQAVMAYHGKIDYFIDTVFNYPTLAECYKV
                     AALDGINRLPRPWAPIP"
     gene            complement(13306..13968)
                     /gene="tpx"
                     /locus_tag="VLD60_12080"
     CDS             complement(13306..13968)
                     /gene="tpx"
                     /locus_tag="VLD60_12080"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HSB45746.1"
                     /translation="MTATVARLFMTLILLTPLSLAGCMGAGYESAFLYKNLPVAEGSA
                     SAGDGHHILFKGSPLALSGNGIKVGDQLRSVRVTLTDLTLVNLVDTTGKGKVRIISVV
                     PSLDTKVCEQQTHHLSEKNKGLDKMVELITVSIDTPFAQKRFAEEAKIHNITFLSDYR
                     NADFGKAYGLFLKDPHILARAIMVVDAHGTVRYLQITPELAQLPNMDEAFQFARKLIT
                     NS"
     gene            complement(13997..14971)
                     /locus_tag="VLD60_12085"
     CDS             complement(13997..14971)
                     /locus_tag="VLD60_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB45747.1"
                     /translation="MSTPAAENIFQHPAGRAYRSLLTFSLLVAMMAACSSSSPIPSQF
                     EAIERLPIKTVSIKGQRMAYLDIGSGQPVILIHGFGGSMWQWEHQQTALSTQVRLLTP
                     DLIGSGLSDKPDIEYSPEQILDHFVAFMDALQIPQATLVGNSMGGGLAIGMALTYPDR
                     VSKLILIDGLPANVLHKLTSPWIRRALETSAPTWLVSFGNWLFGSLMIKSVLKEIVHD
                     PALLTPAVIERSNRNRQRPGIIPPLLMVGKNLPLWEAGFAKRIGEITCPTLILWGEED
                     RVFPIAVGEELHETIKGSTLVRIPKAGHIPQWEQPALANQAMIEFIQR"
     gene            complement(15169..17124)
                     /gene="oadA"
                     /locus_tag="VLD60_12090"
     CDS             complement(15169..17124)
                     /gene="oadA"
                     /locus_tag="VLD60_12090"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HSB45748.1"
                     /translation="MATKRPSAKKQATKKRQAGPVIKTSKGQKPRLQDFAIQPAAGKS
                     ILITDVALRDGHQSLLATRMRTEDMLPIAQKLDAVGYWSLEVWGGATFDTCLRFLKED
                     PWERLRTLRAAMPNTKLQMLLRGQNLVGYRHYADDVLERFVERSAANGIDVFRIFDAL
                     NDVRNVDRAIREVKACGKHAEAAICYTVSPVHNIDRFVDLAKQLEDMGTDTLCIKDMA
                     GLLAPLDAYHLVRRLKAAVKVPIHLHSHYTSGMASMSSLMAILGGLDMLDTAMSPLAG
                     GTSHPATETLVAALKQTPYDTGLDLASFLPITEHFRSVRRKYRQFESDFTGVDAEILT
                     SQIPGGMLSNLAAQLTEQNALDRMKEVLDEVPRVRKDMGYPPLVTPTSQIVGTQATLN
                     VLTGERYKVITNETKNYFLGLYGRAPGQVDRDVMTRAIGDEEPIKTRPADRLDPEMEA
                     VKKDLPASADTVEDQLSFVLFPAIARDFFEAREKGDLVPEPLETTSPKGPAVGGELHL
                     APVEFNVTVHGETYHVKVSGSGRKTEGRKPYYIRVNDKLEEVSLEPIQEILAGLPETP
                     ESGSSAKPKRPKPSKPGDVAPPMPGRVVKVLVAKGDQVKAGDPLLIIEAMKMESRVPA
                     PVDGRVTGILVAEGDSVKTDETVIQLE"
     gene            complement(17172..18590)
                     /gene="accC"
                     /locus_tag="VLD60_12095"
     CDS             complement(17172..18590)
                     /gene="accC"
                     /locus_tag="VLD60_12095"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSB45749.1"
                     /translation="MFKKILVANRGEIAMRIIRACRELDIATAAIYSEADSTGIYVKK
                     ADEAYVVGPGPVKGFLDGQQIVDLALRIGADAIHPGYGFLSENAHFAELCQASGITFI
                     GPSPRAITLMGSKVKARDLAKEVGVPIVPGTDGGVSDVKEALAFAKKAGYPVIIKASA
                     GGGGRGLRVVRSDAELRENMEVASREAQAAFGDGSVFIEKFIERPHHIEFQILADRHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLILTPKLRSEMGKAATTIARAVHYDNAGTVEFL
                     LDQEGHFYFIEMNPRLQVEHTVTEQITAIDIVRNQISIAAGKPLEIHQKDVTLQGHAI
                     QCRINAEDPKNNFMPCTGTVTAYLSPGGIGVRIDGAVYKDYVVPPYYDALLAKLTVRG
                     RTWEETVSRMRRSLEEYVLRGVKTTIPFMKTIMEDQDFLAGRFDTSYLETHPDLFNYH
                     EIEEPEDLVVAISAAIAAYEGL"
     gene            complement(18753..19220)
                     /locus_tag="VLD60_12100"
     CDS             complement(18753..19220)
                     /locus_tag="VLD60_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB45750.1"
                     /translation="MQTWRSSVFMVTGLAAITLCLTVPVGAEESSGQGSIVEGAGFTL
                     FDMESIKGFDAEKVERDPVCDRSKRPRIAKVEPDDVKPGDKVVIKGENFGTKECFHTV
                     TFSASPKATVDYKFVNDSTIEATVPEAKPGMSFLIIVAGGGSAQSKPLLIQAK"
     gene            complement(19249..20121)
                     /locus_tag="VLD60_12105"
     CDS             complement(19249..20121)
                     /locus_tag="VLD60_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSB45751.1"
                     /translation="MRSHHRDDHQKKGAKSLLIVKILSLLAFNILFALTIPFSSLADI
                     AGNLVIAGNGPELSTIESLARAFEKANPRAYVDVRWDDHSKPVDMVKGGDAQLAVTGY
                     EEPGLAATQIAWDGIGILVHLSNFTKEVTKQQIADIFSGKVREWSELGGPETKILVID
                     RPKNQNIRDAFENQLGITGKISDSAKVIGKDDKVVKTVVGTLPPLSAVAFISLGQGLS
                     VVSSGVAVRLLPVDKVEPEVPTVKDGRYPLRRPVLLVSKKEAHPLIEAFTKFALSPAG
                     QTIIAETYVPFDRK"
     gene            complement(20141..20437)
                     /locus_tag="VLD60_12110"
     CDS             complement(20141..20437)
                     /locus_tag="VLD60_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB45752.1"
                     /translation="MPIYEYRCGQCEKTFEATQSVHARVEDTVCPFCNAQEATRLISS
                     FASKVVGTHKPGFTEMKAYDMLNERMDKFAKLPPMIGKRAAPDSTPAPNGESGK"
     gene            20577..20753
                     /locus_tag="VLD60_12115"
     CDS             20577..20753
                     /locus_tag="VLD60_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45753.1"
                     /translation="MPVLIRRYKGHNGMMQEERIDDEDRIERYFRLFEKDDVKKLETG
                     VKVFIEKDEWQLLP"
     gene            20848..21051
                     /locus_tag="VLD60_12120"
     CDS             20848..21051
                     /locus_tag="VLD60_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45754.1"
                     /translation="MTYWIHIARSIDRVTLHKDRCSEVPARHSSSDFWEGGWFDYPDK
                     ERAIAAMEQAGTSDQRRCPLCKP"
     gene            21135..21998
                     /locus_tag="VLD60_12125"
     CDS             21135..21998
                     /locus_tag="VLD60_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSB45755.1"
                     /translation="MPRLALLLTTLIWGATFPATKAALEQIPPLSFLLLRFLLGTLIV
                     AGWFVMMRHRVHRGREVLYASGIATIFLFLGYVLQTVGLRYSTASNSAFITALYVLFV
                     PLILRRFEGRVLLATAIATGGLWLLVKPSASVNIGDVMTLGCALAFAGHIICLERFTR
                     RYDAPSLLLWQMTAMSILFIPTTLWEQAPGAAFAPTAVLLIGLAVTGGLATGAFAVQM
                     WAQQIVPAQQVALVFASEPAYAAWLSWYFLGETLDLQGWIGSGMILLAVLIGAFSSTT
                     SQVASETAQPA"
     gene            22019..22489
                     /locus_tag="VLD60_12130"
     CDS             22019..22489
                     /locus_tag="VLD60_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45756.1"
                     /translation="MAQKFGNGRWVQEGFLDNRVEGTVVGRIVFAAIGPVDLYLKGNF
                     KADIAGQVIQFRNSRFEDDDMAAQVIGDMEIPQIGTVNLISFDPHPNLVPHPYVEWFS
                     TGNNHYRFELNTGEAWIVPEGERSMIDRESRRIREAFSGVSSQRNRSEEDSDWV"
     gene            complement(22566..23540)
                     /locus_tag="VLD60_12135"
     CDS             complement(22566..23540)
                     /locus_tag="VLD60_12135"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSB45757.1"
                     /translation="MSDVRILHPTKAQNLWFTFLRWFDSWAGLEYIKTDGVPKMDWFR
                     CIPIIAVHAMCLGVIWVGTSWTAVTVAVAFYFVRMFAITGWYHRYFSHRTFKTTRACQ
                     FAFAVLGGSCAQRGPLWWAGHHRHHHIASDTPDDVHSPRQGGFLWSHIGWFTSQTHFA
                     PRLKAIADFAKYPELRFLDRFDILVPTIAGFGMFGLGKLLEAYAPELGTNGPQMLIWG
                     FFISTVALIHGTCTINSLSHVYGSQRYETGDDSRNNFFLAMITMGEGWHNNHHYYPAS
                     TRQGFYWWEIDMTYYCLKTMEWLGLVWDIKDVPVYVREQKSKHEAIAV"
     gene            23891..24916
                     /gene="hemE"
                     /locus_tag="VLD60_12140"
     CDS             23891..24916
                     /gene="hemE"
                     /locus_tag="VLD60_12140"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSB45758.1"
                     /translation="MKDRFLKACRREAVDCTPVWFMRQAGRYMAEYRSLRAKHSMLDL
                     CKTPELAAQVTLQPIDRFPLDAAIIFADILLPLEAMGLNLEFAEGEGPVIHNPVRERA
                     DVDRLKVINGSELDYVAEAIRQTRKALNGRVPLIGFAGAPFTLASYAIEGGGSRNYVL
                     TKSMMYCEPEVWHKLMDKLARVVTGYLRRQIHAGAQAIQLFDSWVGCLSAGDYAEYVM
                     PHVQLIFEGLKREGVPMIHFGTGTTAILRQMREAGGDVIGVDWRIHLDEAWTMIGHDR
                     AVQGNLDPVALYAPLHEIERRIEDILRRAAGRNGHIFNLGHGILPTTPVDHVEAAINM
                     IHKLSQR"
     gene            24932..25882
                     /gene="hemH"
                     /locus_tag="VLD60_12145"
     CDS             24932..25882
                     /gene="hemH"
                     /locus_tag="VLD60_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HSB45759.1"
                     /translation="MASSQNPIAVLLMAMGGPDCLENVEPFLLDVRGGRPTPPELVEE
                     IRERYRATGGKSPAVGITKDVAKKLEARLNETGNERYRVYVGLRHWHPFIKETYAELL
                     ADAPEQIIGFCMAPQQSSLSTGAYRKKVEEAQASLQSTCPVSYVGSWNRHPKLIAAII
                     ENIKQGLLKFPADVRAGVPVLFTAHSLPERIVAMKDPYPEEVTGTVEAVTALLGNQPT
                     RFAYQSQGRSGEKWLGPTVESAIEDLSRAGHRHVLVAPIGFLCDHVETLYDIDIELKQ
                     LAASHGMQLERIQMLNDSPALVEVLADVLAAHKSSLTITS"
     gene            25879..27327
                     /gene="hemG"
                     /locus_tag="VLD60_12150"
     CDS             25879..27327
                     /gene="hemG"
                     /locus_tag="VLD60_12150"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSB45760.1"
                     /translation="MSNPCTVAIVGGGISGLSTAFALQERAAAAGLAIRCTVVDAAPS
                     WGGKIVTHRIGDLVTEAGPDSFLSQKPAGLQLVEKLGLADQLINTNETGKKAFVYSGG
                     RLCELPEGLVVITPGQIGPFLKSGLLTVSGLARMGLDLVMPAKRSQEDESLASFFRRR
                     FGRQAFERMMEPLMAGIYAGDAEQMSVNATFPRFPELEREYGSVIRGMMAARKTGRPQ
                     GSATPRRTMFVSLRNGLEDLVDVLVRRLTEQSVTLRSGAVVESLRVRSHQLGRWTYDV
                     MLSDGSALSVDSLVLATPAYVSSELLRPLTPIAGGLLDMIPYASTATIAMAYPDSVIG
                     GGVQGFGFVVPRAEQRDLIAATWTSLKWPHRAPSDRLLVRCYVGGVGREAILQLDDEA
                     LVARVKSELKLICGVTAEPTYVEVNRWMKAMPQYLLGHLERLDQVDAALSRYGGLVLT
                     GAAYRGVGIPDCIRDGAVAADRVIRYLSGERV"
     gene            27349..27762
                     /locus_tag="VLD60_12155"
     CDS             27349..27762
                     /locus_tag="VLD60_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45761.1"
                     /translation="MGTETKMTIVDVSGPFREPRELAFSYDYSIQRMNWPTPHAVRVK
                     VGIPEELDTLRAKILGATTGTPGQQLVISQIMSRRIADEKLRIAEGEGQLLERREVVI
                     PPYAGPLAHMYARLESWVERERETLRAEIMKRANL"
BASE COUNT         5706 a         7641 c         8572 g         5910 t
ORIGIN      
        1 acagtctgtg gggacggtct atggcgatgt ttgtttgtag agcaaggcat gtcctctgac
       61 tggatagacg ttcagattta ctccgcggtt gatgcggggg aggtgctggg attgatcgac
      121 gatcctgctg tgcagggcgc ctggcaggac gggggaacga tccacctcta ttggcccagc
      181 cggaactggt cgctggatca tctggtgcgg cttcggcaaa tccttcgcga tttggatata
      241 acggggcagc cggtgccgga gattctggtt caatcgatac ccaacgaaga ctggaaccgg
      301 cagtgggcac agtcggtcaa gcccttatgg attgcaaatc ggattgtgat caggccgagt
      361 tgggaaacag tgagcctgcg gcctgagcag atcgaaatcg tgctcgatcc gaaacaggcg
      421 ttcggcaccg gccaccacgc cacgacgcgg atgttggttg aatggcttga aaccgtgatt
      481 cagggagggg agaccgtgtt agatgtcggg accggtagcg gcttgctggc catggtggct
      541 cttcgactcg gcgcagcgcg agccctcggt atcgacaacg atcaggtggc gatcgattgt
      601 gccagagagt atgcgcgagc caatcaattt ggcgcggaac tcactctcga atgcggcacg
      661 cttcgtgatg ggatgccatt cgatctcgtg ctggcgaacc tggatcgtca gacattattg
      721 ctgttagctg atcagttggc cgcccgtacg ggaggcagac tcctcctgtc cagtgtgttg
      781 acggatcagc gccgagatat tgtggacgct tttgcgaaag tgcatctcta tccagggcag
      841 cagcgggaac gtgatggctg gctggccatg gaatttatac cagctcaatc ctgtgaggga
      901 gcttgatgac gcttcattca ggccggccgc cgtatcccca tgtacatcag atcaacattt
      961 cggatggagg ggttccgaaa ctgccggtgc ttgaggcaaa ggttactgaa cagggggtag
     1021 cgggcgaccg ccagcgtaat ctcaaggtcc acggtggacc ggatcgagcc gtctgtctgt
     1081 tctcgttgga actcatcgaa cggctgcagg atgaagggca ttccattgat gttggatcct
     1141 cgggggagaa tctcacgctg gcaggactcg attgggaaaa gcttgggccg ggcgttcaac
     1201 tgacgatcgg tccagagatc cgcgtggaaa tcgcgagcta ctgtgcgccg tgtgagtgga
     1261 atgcgcgctg gtttcgtgat ggagagtatt cacggatctc gcagaaaaag aatctgggct
     1321 ggagccgtct ttacgcgagg gtgttgcaag gcggagtcgt caggcccgga gacgaagtgg
     1381 ttattaacaa aagtgactag caatcagctg tcagctttca gccccccatt ccggatgatt
     1441 tgctaatggc tgaatgctga aggctgagtg ctaaaggcag gatctatgga tcgcgtgctt
     1501 gagccggaac tgatggagga cgaacagcaa gttctcgcct atgcgaaggc ggactttgaa
     1561 gaggagaacc agggcttcgt cgatcgattt cgggaatact ttccggactt ttccgaagga
     1621 catgtgttcg acctcggctg tgggccgggc gatattccca ttcgaatcgc caaagtcttg
     1681 ccggcgtgcc tggtgaccgg catcgatgcg tcggccccga tgattcgatt ggccgagcag
     1741 gcggtggccc aggccggtct atccggtcgc gtgacatttc gctgtgaacg atttcaagac
     1801 attgccggcg ccagtcgagc cgatgcggtg ctgtcgaaca gtctactcca ccacgttccg
     1861 aacccgttgc agttctggaa tcgtctccgg caactcgtca agcctggttc gccggtcttg
     1921 gtcatggacc tcctcaggcc ggagacaccg gaagaagcgc aggccatcgt ggaccggtat
     1981 gcagcgggag agccggaaat tctgaggagg gacttttaca actcgctgct cgcggccttc
     2041 acggaagatg aagtaaccac gcaattggcg cgcatgaatc tcacgcggct cctgatcgat
     2101 gttccggatg atcgccattg ggtggtcgga ggcctcatcc attgaacgtt gccgcataca
     2161 gggcactctc ggccctgtgc ctgcttcttc tattcggact ccacgtcgaa tcctcattcg
     2221 ctcaattgga ccggctgcgc aaatcgaagc cgcttgatgt gcaggcgcct ggcgaaaccg
     2281 ccatggccga tattccggaa gggttttttg tgatggggtt cgacgggacg caggcgctgg
     2341 aggatgaacg accgagccat cgagtctggc tcgatacctt tgcgatggat gtgcatgagg
     2401 tgacgacggg gcagtacgcc gcgtttcttg ccgcgaccgg tcgcaccgtg ccttggcagt
     2461 gggaaacggt ggatttgagt caacacaggg agcgtcctgt cattggcgtg gattggcacg
     2521 atgcgcaagc ttactgccaa tggaagggaa agcggttgcc gaccgaagcg gaatgggaaa
     2581 aggcggcgag aggaacggat ggcaggctct atccctgggg caatcaggtg ccgacgaaag
     2641 aggtggcgaa ctttgcacta ggcgcacggt tcagctacag ccaggtgctg ctgccggtgc
     2701 ggtcatacga gcagggcaag agtccctacg gtctctacca gatggctggc aatgtgtatg
     2761 aatgggtgca ggactggtat gcaacgaact attacgacac gtcgccggat cgcaacccgc
     2821 aggggccagg acaagggcaa ttcaaagtca tgcgcggcgg gtcctggtcc gacctgccta
     2881 aatatctcct gacctacggc cgcttcaagc ttcctcctga gacgcggaat agctataccg
     2941 gtttccgctg cgccaaatag tcggatcctg cctcacgcag actgcgcagt ttcgcagctc
     3001 accgcaaaat tgtttgttgg gcgggggttg agggcgcagc ggtcccgtca ggtcagggca
     3061 agcgcgagaa gaaagatgcc ggtcaatgtg cagagggagg aaaggtagag gattccggtg
     3121 cgatcgccga tccaggctgt tttccatttg cccggataga gggcctggtg ttcgacgaat
     3181 aatccctcct cgtagaatcc cagtgcttgc tcaagttgga tcaagacctg tttcgcgagc
     3241 cggtggcgat cttgttgctg gaggatcagg ccgcaaaaga gtccgcacca gaccatggcg
     3301 gcgagagcca gtaaaaacga ttccggtggc gagagccggc tcttgagggg gctgaggacc
     3361 agcgagaagg tcatggcaat caggccgagc gaaccgactg ccgtcaatcg catcatctgc
     3421 tcgcgccgtt tgtagacttc ggctttatac agcggataga gcacggtaag aatttgttgg
     3481 gtattttcgg tgatcatatc aactggccgt taagacgtgc gtgatggaat cagatgcatg
     3541 gcgagccatt ccgtgatcga cgtcgtcatg gcatgaaaat cttcggctcg cgtaaattgg
     3601 tgatcggctt ccggcaagag gtcgatccgt ttcggacctc tcaggaggtc gaagagcttt
     3661 tggctctggt gaagcggcac cagctcgtct ttttgcccct ggacgatcaa cgtgggagtc
     3721 gtgatgccag tggccgggcc gtacgccgcc aggtgcaggc agtcttcata gagccggtac
     3781 ttcagacgga cccgctccga ccctcccatg atgttctgaa tggtgtccgt tgcctgccag
     3841 cgcgccattt catccggccc aaattctagg cgaagctctt ccgcaaaatc caccacgggg
     3901 cactttaagg ccaggcaggc aatatcctgc cgttgagcgg cggtgagtat cgcgaccaac
     3961 cctccgaagc tcgagccggc aagtccgatt gcgttgtaac ccattgagag gacgttgttg
     4021 atagctgcgt tcgcttgtcc aacggccagc gtcgttgtga tgtcttcgaa gtgtccttcg
     4081 ctctctcctt gaccgaaaaa gtcgaaccgg tacgtggcaa tgccgcgggc gttcagcagc
     4141 ctggtcaacg tcttgttcgt cgtgctgttc ttgttcgacg ggaacccgtg gcagaggacg
     4201 acgatcctgt cggtgtgctg ctccggccgg ctgagcacgg ctgcgacgcg atgtccccgg
     4261 tcgtcatgaa acgtcaaagc ttcttccata aaggtatcgt cttctcgtgt ggcgatggag
     4321 tgcttactgt ggagggcgag tgcccagagc ccaggcgagc tttgccgcga agaacgcgag
     4381 ggagatgaga agaaggccaa aggttatcca ggtcgccgct tggatggcgg gagcaccgag
     4441 cggcatcttc catgtcaagg acgccacgag cgcgagcccc aaggtgccga ggctcaatcc
     4501 gccaatgtgc acgattcgcc agcggttgat aatagtcgtg agcgtcgcga gatatggctc
     4561 gcgtttctta tggttgggaa cccggctgac ggcgagcgtg atcgggacca aatgcgagag
     4621 ggcaccaaaa attgtctgta acacgaagcc gaccagcgcc atgtgcgtat aggccacaac
     4681 gtgcaatgtg ccgaacggga agaccgggac gtcccagaga gagttcactc cgaccagcag
     4741 ccccatcacc atggcgagaa cgaggaaaaa tgtcgcgaca agcagatgat ccgaggcggc
     4801 gttgcctgcc tggttggagg ttttccagga cgcaaagagg ttaaaggcat agagtcccgt
     4861 cccagcgacg agcaggattc ccgccgcgag ttcggttttc agcgaggaca gagcaaatcc
     4921 tgcgatcagc acagccatac ctgttggaag caggaagaac accgcccgcg cgagacgcgg
     4981 gctgtagagc ttgttctgga ggatggtggg aagcaggttg tgcatcgtcc ccacgatcac
     5041 cagcgtgacg aacccgagga gattgagatg gatatgaacg agccgcgcat ggccatacca
     5101 ggacatgtcg atgaatcgag aggccatgcc gatcccgacg gcgagaccgc cgaacagcgc
     5161 gatcagggcc aatccataga acaacagatt caagggtggc gaattgaggc tctgtcgagt
     5221 ttgtcgccag gcgtcgctcg cgattgagag aaaggctccg aggacaagga gtccgcctcc
     5281 tgcgacgatc gcgaagttct ggaatccgaa tcccgtcacc atccccagcg cgccgccgtt
     5341 gacgacagcc aggaggatcg gatgcgagtc cgatcgcttc tgcccggtca tgagcagcgg
     5401 gggaataaag gccagcatcg cgcccatgat catctgtgca acgccgccga ccaaggcccc
     5461 gtggacgtgg acgagcctca accaggaggg aaggggggtg cctcgtatca gaccgaccag
     5521 aatcgccagg ccgatcaatg aagaaaggag cagccaggta aagccggtga ggagaaacgt
     5581 caacggggta cagcggacgc tgaccatgat gagttaccct ccaagaaaat agaagtgatt
     5641 ggtcggtccc gtcccatgac cgatcgccag gctatgacgg atggcttcgg tcaaataggc
     5701 tttggcggtc tgcaccgcgt cgttgagcgg tttgccgcgg gccagatgag ctgcgatggc
     5761 cgaagcaaag gtgcagccgg tgccgtgggt atgaggcgtc tcgatgaact cgcctttgaa
     5821 cacgtgaaaa aagcgcccgt cgtagagcag atctgtggcg cgttcggcga ggagatgccc
     5881 gcctttgatc agcacgtgct tgcagccgaa tccgtgaatc actttagcgg cgcggcgcgc
     5941 gtcggcaagc gctttgattt cgatgccgga gagcagctgg gcctcgtgga tattcggcgt
     6001 aacgagtagg gccagaggaa agagctgctt cttcagcgcc tcgatggcct ccggtttcaa
     6061 cagcggatgg cccctcttcg agatcatcac agggtcgacg acgagattgg ccacattctg
     6121 cggtttcagc atcttggcga caagttcgac gacggccgtg gatgacaaca tgccggtctt
     6181 gacggccgct acttcgaagt cgtcgaacac cgcgtcgatc tgcgccgcga cgatcgaggt
     6241 aggcaattcg aagatgtcgg tgacctcttc tgtgttctgc gccgtaatgg ccgtgattac
     6301 ggacatggcg aagacgccgt tggccgacat ggccttgatg tcggcttgaa tacctgcacc
     6361 accaccggaa tccgagccgg caatcgtcaa gacttgtttc agggttgtgc tcatctgtga
     6421 ggtgtatcgc tccttagggc tggagaagca acgaatatat atactttgca atgcggcgtt
     6481 gtcgagcctg tgctatcgga agatgagact gcccaagccg cgcccaagcg cgtaggcgat
     6541 gggcatgagc cccaacagca cgaaggggag aaagaggtag gcgagggctc cctgagcatc
     6601 cggaaaccag agcgtcatga catgagtcca ggcactgagt ccgatcatcc cgattgccgt
     6661 caccaggacg cctgctgcac gccggccgca taatttacgg tccagcgccg gggcgagccc
     6721 catgtggaag agtacgaaga tggcgaacag cacaaaaggc accgcgttca ataacccgac
     6781 gagaaagata ttatagcccg gggcaaagag atgcaggtcc agatgatgcg ccgcgtccgc
     6841 aagcgactgg ttgtgtttca gaacatccac ccccatcgtg gccagccagg ggattccgag
     6901 gccgagcaga atgcagcccc agtagaccag catggtgaac agtgtcaggc gatgcgttga
     6961 ttggtccgtt gtcatggggt tgtgctcgct gtacgcgtga tcgattcgag agccgctgcg
     7021 acagcttcgc gaacaaccgg atcagcatcg tggagagctt gttggagcgc cggaaccgcc
     7081 tctttcgctg ccggtccgat cgattccaga gtgctcgcgg tgaggtatcg ttccagcggc
     7141 agctctgaca taaggagcga tgtcagcttg ggaactgctt cagctgcgcg gggcccgagt
     7201 ttcgccaact ggagcaaggg gtaggtgcgg tcgactggct ggtcgagcat caagagttgg
     7261 accgggatgg aagtcggccg ttctgccgcc gtgacagccg actgggccaa tcgagcgact
     7321 ggttgattcg aatctttggt gagatcccgc agggccgcga gcgattcggg actggaaaat
     7381 ccgctgaaag aaaattgcat aacggcggca taacgcaccg tccattctgg atcgcgcagt
     7441 gcctggagca aaccgcccat gaccctgtcc gtcggaagga cgattgcgcc caacgcacgc
     7501 acggtccgtt cgcgcaccac cgtatcggaa tcgttcaata ttcctatgag tccttgaaca
     7561 gccggtcttg cgaccggacc caatgcgcca agcaccgaag ccgcatcgcg tctgatctgc
     7621 gagtccggat cttgaagctt cgggagataa tgcaccatga cccgtcgcgc cgcactcaag
     7681 tcgatcgtcg ggagcgcacc ggccgcctca ctgtgaagcg aagatctccc ggctgatgtc
     7741 gccaactcga gcagcgccgg aaccgccgcc tgcgccggaa gaccgatatg cttcaacccc
     7801 tccgccgcca gcatctgcgc ctgccgatcc tggctctgaa gatcttcaat caaccccggc
     7861 accgcccgta tacccgtatg ttgccaatag accaatccca gaagcgccat cagcacccct
     7921 agcccgccaa ggttcagcca aatttgacgc tttgtcatag gagaaactct atcagaaagg
     7981 tgttgggcga gacataaggt ggtctcatcc aaccttcgag agtgctttgc cgatcgattg
     8041 ctctcgtggt ttagcgtgct ccttaccaac cgagggttgc ttctgtgaag ctgttcgccg
     8101 tgattagttt gtttgctgtt aattagtttg tttggtggtc ttatacagtt cgagtttgac
     8161 atgcagaacg aaaatcctcc tactaaaagg aaacagtccc tagagaaagt cttgctcgca
     8221 tggtaggctg cgggaggata gttctgtatg ctcacgaggt gaccacgatg acggttggag
     8281 acccgatgtg tactggtatg aggaccagcg cgatctggat cggatggatt gctggcctga
     8341 cgctggtcgc gggcggcctt ctactgatgt tcggttgctc gacggcatcc gctcctcccg
     8401 cgaacaccga ggccgtgcgg caacatgccg acaaggcctt tcatgattcc aaggcacagg
     8461 tgcagcctcc gaaacaaggc gatgcacaat ccccaccagc cgtgtccggc acagacgatc
     8521 ctgcaaccct ggcgtcgcca tccgttcaaa agggagtaca gattccgctc gctgagattg
     8581 gcccgtctga tggcgagact atccgtgcca caggctatgg cccgctcgcc aaagggctca
     8641 ccctctgcca gcattcggcc gatctggccg cacgggtcga attatcgaag ctggtccgag
     8701 tccaggtcac agaaaaatcg gtggatcgcg tccgtgaacg gaccggcaag aactttgagc
     8761 aggatattga agtggtccgt gagggcttcg tgaatgaagt tctcagcgac gtccggatcg
     8821 tcgaccgtaa ggtggacaag gaggccggta catgctccag taccgccgtc attcccaaac
     8881 ggaatctgtt cccgacgtcg ggtaatcaaa cactagaggg tcggcccatc agcaaataac
     8941 ctccgaaagt ggtcagatta gaaagagcga ggggtccttg cgggcagaaa catcgaggga
     9001 tatgctaatg atcggtataa gagggaacgg catgacgcca agaattgtgc tgctcgtcat
     9061 ggcgacactc ctgggagtgt ggccgagtat cggcgtggcg ggagatctga cgccgcgcga
     9121 gatttacgag cgcacgtcgc cagccgtcgt gatgatcatc gggtattcca acagcggcca
     9181 acggggcagc ggtggcaccg gttctatcat ccaagaggat gggctcattt tgacaaacgc
     9241 gcatgtggtg attgaagagc agacaggcag gccctatcca cgcctcacca tctatttgaa
     9301 gccagaccat gtgaccggcg accccaaggt ggacttgtcg cgcgggggca aggctcgtgt
     9361 ggtggccttt tcacagacgc tggacctggc gctcctcagg ctcgagggaa tgcccggacc
     9421 atttccggcg cttggactgg atgactcaga taaaaccgag atcggcgatc gcgtggtggc
     9481 gatcgggcat ccagaacagg gcgggctctg gaccttgacg accggcacca tcagcgcgga
     9541 gttcgaaaac tttaacgcca ccaagggcaa gtccgtgttt cagacggaaa cgggactgaa
     9601 ccgcggcaac tccggtggac cgttgctgga tacccacggc cgcatgatcg gggtgaatac
     9661 ggctatcgtg cgcgtcgcgg cggatgggct tcccattacc agcatcagct tctcgttgaa
     9721 gtccaacgtc gccaagcggt ggttgggtga gcagggtgtt tcggcccagg aggcgctggg
     9781 tccctccgac acgccgggac tggacaaatc tgcgagtcgt tctacctccc ggcctagcgg
     9841 gcaaacgatc ccgccgacta atccgccggc cgctcccacg atcgtcgctc catcttctcc
     9901 cactgaggcc gctgcacaag tcccttcccc tcgaccatac aatctggacc gcgtcatcag
     9961 tgaacggagc cgcgccgagg ccgacctgga agatatgatc aaggaaatgc ggggcaaact
    10021 gagcgggcga tgaggacctc gccgttcact tctagcccag cctgatcaag aactgctcgt
    10081 ccgacgtaca agaagtccac agcgtgtcct tagactacga gatggcggtg agaaatggtg
    10141 actcgcggcc tctttctcaa caagggtcgc cgtatcgact gccgttactt caggttcatc
    10201 gcctgatagt caatgaggaa ccgtccgaca gcatctaaga ttctggcgcg aatgtcggga
    10261 actcgagggg cactaagcga actaggtggt gcagagccct cccacgtggt cgcaaagaat
    10321 tctgtcgttg gatcccgtga gaggtgaaca agttgtttca acgtgagccc gacagcgtac
    10381 gcgttgaaat tcactgaggg agctgtgatt gggtagacag cgacataaag atagggggcc
    10441 ccgggtgagt tcgctatctc ggagatggtc aggacgcgca ggccagccag tccaaggcgc
    10501 gcttcgacgt cgtgtttgag gtgttctcgg ctcaaccctt cccgttcaac atcgtcagga
    10561 agatactcaa cgagcaccga aacctctctg agtcctcgca acgtgtcacg tttatccgcg
    10621 gtaaaggcga atccgacaat cggccaacac aacgtcagac ccaacagcat aagtatgctc
    10681 cggcgcatcg gccacctcct cgtcagtggt ccgcacgata gccgcccgcc tagataaaga
    10741 caagagcaag acttgagtgg cgaagctcgt ttaggaaggg gaccctatca ccacagctga
    10801 tcccgcgtcc aaaccgccgt ttcttatgac gtacgtttct ccacgcgcaa ccctgaacaa
    10861 agagaaagaa gtccttatga ctcgatccca agagcgttcg tctaaccatc tctgaaccca
    10921 atgaggtggt ttcggaatct cactcccgtg agtaaagggt cacctgcact ttcctctctt
    10981 cctcaatttc attcgcaagg tgttctacgg gtggcgttga cctgggagcc cgtccgcccg
    11041 cagcggtgtc tccctacacc gggcggggtg cgaggacgga cgggttggcc agcgacagga
    11101 cccgtcgact cacaccggcc acttctcctc gttccacgcc atttcccaaa acatccactc
    11161 atacctcgag ctggtgatga atgcctcctc catccgccgc ttttcttcct ttcccgccgt
    11221 cttcgcccac tgatccacct ttttcctcat ccattgctgg acttctgcaa attccggcga
    11281 ggcatagagc atgagccagt cccgataggg atgtttcttt gccggcggtc cgttcttcag
    11341 caagtgttga ccgacgacac aatagatcca ggcgcagggg agagccacga cggtgatttc
    11401 tgcggctgat cctgaggcgg cgacgtgcag catgtggcgg gtataggcgt agttggtcgg
    11461 cgccatcggg acggaggaca tttctttagg ggacaaattc caacggctgc cgtagccttc
    11521 atgcaggctc cgttcgacgg tgatggtctc ttccgcgagt ttggcaaaac gaagcgcggt
    11581 ttcagaatca ggtgcccgca tagcccctgc cgcaaagacg cgggataggt cgccgagaaa
    11641 gcgcgcgtct tgcaggatgt agtatttgaa tctctgttcc ggcagcgtgc cgttgcccag
    11701 agcgacgacg aacgggtggt tgagttgcgc atcccagatg ggcgtcgcca gttgtcggag
    11761 gcgatcagaa aacaacatgc gggactcctt tgtcgttcgg ggaacaggtt agggaatcgg
    11821 cgcccagggg cgcgggaggc ggttgatacc gtccagcgcg gcgactttat aacattcggc
    11881 gagtgttgga tagttgaaga ccgtgtcgat gaaataatca atcttgccgt ggtaggccat
    11941 cacggcttgg ccgatatgaa tcagctccgt cgcgccttca ccgatggcat gtacgccgag
    12001 cagttcgtgg gtgtgccggt tgaacaggag cttgagcatg ccgctctcgt cgccgatgag
    12061 ttgtcctctg gcgatctctt tgtagcgggc gatgcctacg ccatagggaa tctccgcctg
    12121 cgtgagttcc tcttcattct tccctaccat ggagatttca ggaatcgcgt agatcccgta
    12181 tgggagcagg tccgtatcgg tgcgatccgg atagccgaat gcatggcagg aggcatgacg
    12241 gccctgttgc atcgaggtag aggccagtgc cggaaagccg atgacgtcgc cgacggcata
    12301 gatatgcggg acggctgttt ggaagtgcgc attaacggcc aaacgtccgc gccgatccgg
    12361 cgtgagaccg accgcctcga ggttgagtcc ggtggtcgcg ccgacgcggc cgatcgcgta
    12421 catcaacgtc gtagcctgga tcggtttcgc ctgttgaagg cagacatcga tgcggttgtc
    12481 ggtgtccttc ttgatcgcca gcacctcttc ctcgtggtac agcgtgacgc cgatgtcctt
    12541 catctgccgt tgtagcgccg cgataatctc ggcgtcgacg aattccaaga ggcgcggccg
    12601 tttgtcgatg agcgtgaccg gcacacccag tgccgccaga atcgaggcat attcggtccc
    12661 gatgacaccg ccccccacaa tcatgatgga atcggggatg cgcttcagcg tcagaaggcc
    12721 gtccgtatcg atgatggatt cgccatcgaa tggaatctca gccggtctgg ccggttcggt
    12781 gccgacggcg ataacgatga agtccgccgt atgttcaatc gaaccgccgg cctgctgaat
    12841 ctgtagacag tgcggatcga cgaaccgggc cgtgccgaag atcatatcga cccgattgcg
    12901 agtcatttgg ttttgaacga tggcgatttc gttcttaatg acgtgattgg cacggaaggc
    12961 gaggtcttcg atagtaatgg tgtccttgac ccgatagccg gcgccgtaga gattgcgttg
    13021 gcgaaagccg gagaggtaca gcaccgcttc acgaagcgat ttgctgggga tcgtgccggt
    13081 attgatgcag accccgccga ccacttcctt ccgctcgatg atgccgaccg ttttgttaag
    13141 cttggccgcc tgtatggcgg ctttttggcc tgcgggtccg gtaccgatga cgagaagatc
    13201 gtaatgagcc atgatccctc gagagcaggc tgaggcaatg gttgagcggg agctagtggt
    13261 cgcttccctc gttagacctt agcctcaacc tgatccatcc gcaagttagc tgttcgtaat
    13321 cagcttgcgg gcgaattgaa aggcttcgtc catattaggc agttgggcca gctccggcgt
    13381 gatctgaaga tagcgcacgg taccatgggc atctacgacc atgattgcgc gcgcgaggat
    13441 gtgaggatct ttgagaaaga ggccgtaggc cttgccgaaa tccgcgttcc ggtaatcgga
    13501 gaggaacgtg atgttgtgaa tcttggcctc ttccgcgaat cgtttctgcg cgaacggggt
    13561 gtcgatgctg acggtgatca gttcgaccat cttgtcgagc cctttgtttt tctcgctcag
    13621 atggtgagtc tgctgttcgc acactttcgt gtccaacgat ggcacgacgc tgataatccg
    13681 caccttccct tttcccgtcg tgtcgacaag gttgacgagc gtgagatcgg ttagggtgac
    13741 cctcacgctc cggagctgat cgccgacctt gatgccgttc cccgagagcg ccaagggact
    13801 gcctttgaag agaatgtgat ggccgtctcc ggccgacgca ctcccttctg ccacggggag
    13861 gttcttgtaa agaaatgcgg attcgtatcc ggcgcccata cagcctgcca ggctgagagg
    13921 ggtaagcaat atgagcgtca tgaacaggcg cgcgacggtt gcggtcattt gtgacctcca
    13981 catgagaaag aaacggttaa cgttggatga actctatcat agcttgattg gcgagagcag
    14041 gttgttccca ttgggggata tgcccggctt tcgggatgcg aaccagcgtc gatcccttga
    14101 tcgtctcgtg cagttcctcg ccgactgcga tgggaaacac ccgatcttct tcgccccaca
    14161 gaatcaacgt cgggcaagtg atctctccaa tccgcttggc gaacccggcc tcccatagag
    14221 ggaggttttt accaaccatg aggagcggcg gaatgatgcc gggacgttga cggttccggt
    14281 tggatcgctc gatcaccgcc ggcgtcaata gcgccggatc gtgcacgatt tctttcagca
    14341 ccgatttgat catcaggctg ccgaaaagcc aattaccgaa ggagacgagc caggtcgggg
    14401 cgctggtttc caatgcccgc cggatccagg gactcgtgag tttatgcagc acgtttgccg
    14461 gcagcccgtc gatcaggatg agtttggata cgcggtccgg ataggtaagc gccattccaa
    14521 tggcgagccc gccccccatg gaattgccga ctaacgtagc ttgggggatt tggagcgcat
    14581 ccatgaacgc gacgaagtgg tccagaatct gttccgggct atattcgatg tccggtttgt
    14641 ctgagagtcc cgagccgatc agatccggtg tgaggagccg aacctgtgtc gagagagcgg
    14701 tctgctgatg ttcccactgc cacatggagc ccccgaagcc atggatcaga atcaccggct
    14761 gaccggagcc gatatccaaa tacgccatcc tctgaccttt gatagagacg gtcttgatcg
    14821 gaagacgctc gatggcttcg aactgagaag gaatcggaga ggaggaggag caagccgcca
    14881 tcatggccac gaggaggctg aaggtgagaa gcgatctgta ggcgcgtccg gcagggtgct
    14941 gaaaaatatt ttcggcagcc ggagtcgaca caacgtcaag cgttcacagt tggtctgcga
    15001 gcgcccccca agatgttcaa aagggccgtc cggcgaggcc gcagcgagtg aagaggcgaa
    15061 gcgtgctctg ttctgtacgt tgagcctctg agcgacacga gaatgacgct ggcggatttt
    15121 ttcaacatcc tgctaaacaa tcgagttggg agaaggcggc ggctcaggct attccaactg
    15181 gatgaccgtt tcatccgttt tgacactgtc cccttcggca acgaggatgc ctgtgactct
    15241 gccgtcgacc ggagcaggca cgcggctttc cattttcatt gcctcgataa tcagcagagg
    15301 gtctccggct ttgacctgat cgccttttgc caccagcacc ttgacgacgc gacccggcat
    15361 gggcggcgcg acgtcgcccg gtttcgaggg cttcgggcgt ttcggtttcg cgctgctgcc
    15421 gctttcaggt gtttccggca ggccggcgag aatttcctga atcggttcga gcgagacttc
    15481 ttcaagcttg tcgttgacgc gaatgtaata gggcttgcgg ccttcggttt tgcggccgga
    15541 tcccgacacc ttcacatggt acgtttcccc gtggactgtc acattgaact cgaccggcgc
    15601 gaggtggagc tcgccgccga cggccggccc cttgggagac gtcgtctcca gcggttccgg
    15661 gaccagatcc cccttttccc tcgcttcgaa gaaatctcgc gcgatggcgg gaaacagcac
    15721 gaaggagagt tgatcctcga cggtatcggc tgacgcaggc agatccttct tgaccgcttc
    15781 catttccgga tcgagccggt cggctggtct cgtcttgatc ggttcttcat caccgatggc
    15841 ccgcgtcatc acatcccggt cgacctgccc cggggcacgc ccgtacagcc ccaggaaata
    15901 gtttttcgtt tcgttcgtga tgaccttgta gcgctcgccg gtcaacacgt tgagggtggc
    15961 ttgcgtgccg acgatctggc tggtcggtgt gacgagcggc gggtatccca tgtcttttct
    16021 cacgcgcggc acttcatcga gcacttcttt catccggtcc aacgcgttct gctccgtcaa
    16081 ctgcgcggcc aaattggaaa gcatgccgcc ggggatttgc gaggtcagga tttccgcgtc
    16141 gacgccggtg aagtcgcttt caaactggcg atattttcgg cggacggatc tgaagtgttc
    16201 cgtaatcggg agaaaggagg caaggtccag tcccgtgtcg tagggggtct gcttcagcgc
    16261 ggcgacaaga gtttccgttg caggatggga tgtgccacca gccaacggcg acatggcggt
    16321 gtcgagcata tcgagcccgc cgaggatggc catgagtgac gacatcgagg ccatgcccga
    16381 cgtgtagtgc gaatggagat gaatgggtac cttgaccgct gctttcaagc ggcgcaccaa
    16441 gtggtaggca tcaagcggcg ccaagagccc cgccatgtcc ttgatacaga gcgtgtcggt
    16501 gcccatatct tccagttgct tcgccagatc cacgaatcga tcgatattgt gaaccggact
    16561 gaccgtatag cagatggccg cttcggcatg tttgccgcag gctttgacct cgcggatggc
    16621 tcgatctacg ttccggacgt cgttgagcgc gtcaaatatc cggaacacgt cgatgccgtt
    16681 ggccgcggag cgctcgacga accgttccag cacgtcgtct gcatagtggc ggtaaccgac
    16741 caggttctgg ccgcgcagca gcatctggag cttcgtgttg ggcatggcgg cacggagggt
    16801 ccgcagccgc tcccagggat cttctttcaa gaagcgcaag caggtgtcga aggtggcccc
    16861 gccccagacc tccagtgacc agtagccgac ggcatcgagc ttctgggcga tcggcagcat
    16921 gtcctcggtg cgcatgcggg tggccaggag cgattgatga ccatcgcgca gggcgacatc
    16981 ggtgatcagg atcgacttgc ctgcggccgg ctgaatggca aagtcttgca gccgcggttt
    17041 ctgacctttc gacgtcttga tgacaggccc ggcctgacgt tttttcgtcg cttgtttttt
    17101 tgccgaaggt cgtttggtcg ccatggtgtc gttgtgtgcg ggagcgcgtt acagactagt
    17161 ggtgttgccg gttacaaccc ttcgtacgcc gcgatcgcgg cagagatcgc cacgaccaag
    17221 tcttccggct cttcgatctc gtgatagtta aagaggtcgg ggtgtgtctc caaatacgag
    17281 gtgtcgaaac gtcccgccaa aaagtcctgg tcctccatga tggtcttcat gaatgggatc
    17341 gtggttttga cgccgcgcaa gacatattcc tcgagcgagc gccgcatgcg gctgactgtt
    17401 tcctcccagg ttcggccccg caccgtcagc ttcgccagca gggcatcata gtaaggaggc
    17461 acgacgtagt ccttgtagac cgctccatcg atgcggacgc cgataccgcc cggcgataag
    17521 taggctgtga cggtgccggt gcagggcatg aagttgttct ttggatcttc cgcattgatc
    17581 cggcactgaa tggcatggcc ctgcagtgtc acatccttct gatggatctc gagcggtttt
    17641 ccagccgcga tggaaatctg gttgcggacg atgtcgatcg ccgtaatctg ttcggtcact
    17701 gtatgttcga cctggaggcg gggattcatc tcgataaagt agaagtggcc ttcctggtcg
    17761 aggaggaatt cgacggtgcc ggcgttgtca tagtgcaccg cgcgggcgat ggtggtagcg
    17821 gccttcccca tctcgctccg aagcttcggg gtaagaatca gggacggcgc aatttcgatc
    17881 agtttctgat gccgccgttg aatagagcag tcccgctctc caaggtggat gatgttgcca
    17941 tggcggtcgg cgagaatctg gaattcgatg tgatgagggc gttcaataaa tttctcaatg
    18001 aaaacgctgc cgtcaccgaa ggcggcttgc gcttcccgtg aggcaacctc catgttctct
    18061 cgaagttccg catcggaacg gacgacgcgg agcccgcgtc cgccgccgcc ggcactggcc
    18121 ttaataatga cgggatagcc ggccttcttc gcaaaggcga gcgcctcttt gacgtcgctg
    18181 acaccgccgt cggtccccgg gacgatcggg acgcctactt ctttggcgag atcgcgggcc
    18241 ttgaccttgc tccccatcag ggtgatggca cgaggggagg ggccgatgaa cgtgatcccg
    18301 gaggcctggc agagttccgc gaagtgggcg ttttcagaca ggaatccata gcccggatgg
    18361 atggcatccg cgccgatccg cagcgccaga tcgacgattt gttgcccgtc aaggaagccc
    18421 ttgaccggtc ctggtccgac gacatacgcc tcgtcggctt ttttcacgta aatgccggtc
    18481 gaatcggcct cggaatagat ggcggctgtc gcgatatcga gctcgcggca ggcgcggata
    18541 atccgcatgg cgatctcacc gcgattggcc acaagaattt tcttgaacat gtccctctgg
    18601 cttcccgtac cgcgcacgtg tcgctcccgc tggaccggcg caaaggagcg cgtaagctag
    18661 cataggatca agagtcctgt cgagggagga tcgagcgagg tggagaggaa aacgggcccg
    18721 ggagaccgca cggcttcccg ggcctgaagg ggttacttag cctgaatcaa caacggcttg
    18781 gactgggcgc tcccaccgcc tgccacaatg atcaggaacg acatcccggg cttggcctcc
    18841 ggcacggtcg cttcgatcgt ggagtcattg acgaacttgt agtccactgt ggccttcggc
    18901 gacgcgctga atgtgaccgt atgaaagcac tccttcgtgc caaagttctc gcccttgatg
    18961 acgaccttgt cgccgggctt gacgtcgtcc ggctccacct tggcaatccg cgggcgcttg
    19021 ctgcggtcgc agaccgggtc gcgctccacc ttttccgcat caaaaccctt gatcgattcc
    19081 atgtcgaaga gcgtaaagcc ggcaccctcg acgatggagc cttgtccgct ggattcttcg
    19141 gcaccgaccg gaacggtgag gcatagcgta attgcggcca gccccgtcac catgaagacc
    19201 gatgatcgcc acgtctgcat aggagcctcc ttcatgaaag accgacgatt atttccgatc
    19261 gaacggaaca taggtttcgg caatgatcgt ttgccccgcc ggtgacaatg caaatttcgt
    19321 aaaagcctcg atcaggggat gggcctcttt cttcgagaca agcagcaccg ggcgtcgcaa
    19381 cggataccgg ccgtctttca ccgtggggac ttccggctcc accttatcta cgggaagcag
    19441 tctgacggcg acgccactgg aaacgactga gaggccttgc cccaatgaaa taaacgctac
    19501 ggctgagagc ggcggcagag ttcctaccac cgttttgacc actttgtcgt ccttgccgat
    19561 gactttcgcg ctgtctgaga ttttcccggt gatgccgagt tggttctcaa acgcatcgcg
    19621 gatgttctgg ttcttcggtc gatcgatgac gagaatcttg gtctctgggc ctccgagttc
    19681 agaccattct cgcactttgc cggaaaagat gtcggcaatc tgctgcttcg tcacctcttt
    19741 ggtgaaattg gacagatgga caagaatgcc gatgccgtcc caggcgatct gcgttgctgc
    19801 gagccccggt tcctcgtatc ccgtgacggc cagctgtgcg tcgccgccct tgaccatgtc
    19861 aaccggcttg gagtggtcgt cccagcgcac gtcgacatag gcacgcgggt tggccttctc
    19921 aaaggctctg gcgagtgatt cgatcgtcga cagttcaggc ccattgcccg caatgaccag
    19981 attgccagcg atgtcggcca aagaagagaa cgggattgtc agagcgaaaa gaatattgaa
    20041 tgccagcagt gacaggattt tcacgatcag aagggatttc gctcccttct tctgatgatc
    20101 gtctctatgg tggcttctca cgtcccggcc tgtgtcttgg ttattttcct gattcgccgt
    20161 tgggggccgg ggttgaatct ggtgcggcgc gcttgccgat catcggtggg agtttggcaa
    20221 acttgtccat gcgctcattt aacatgtcat acgccttcat ttcggtgaag cccggcttgt
    20281 gggtgccgac gacttttgag gcaaaagacg aaatcagtct ggtcgcctcc tgcgcgttac
    20341 aaaacgggca caccgtatct tcaaccctgg cgtgaaccga ctgggtcgct tcaaacgtct
    20401 tttcgcattg gccgcatcga tattcgtaga ttggcatggt ggtacgtccc tttttcgtga
    20461 cctcactgac agcggctctg gaggaccctt gaccgctcct cgaacgatgc cgtattgtag
    20521 cacacctgcc tggtttgaag actaggggat tgctcagcgg acgaggagga ggttcgatgc
    20581 cggtgttaat tcgacggtat aagggtcata acggcatgat gcaggaagag cggatcgacg
    20641 acgaggaccg gatcgagcgc tattttcggc tgttcgaaaa ggacgatgtg aagaagctgg
    20701 agaccggggt caaggtcttc attgaaaagg atgagtggca actccttccc taagccagtg
    20761 agaggtaacg ggtgaggagg aaaaagttgc cgaacctttg ctttcatacc cctcacctct
    20821 gacgcttcac ccctaacgat tcccacaatg acgtactgga ttcacatcgc tcgctcgatc
    20881 gatcgggtga cgctccacaa ggatcgctgc tcggaagtgc ctgcacggca ttcctcgagc
    20941 gatttttggg aaggcggctg gttcgactat ccggacaaag agcgagccat tgcggcgatg
    21001 gaacaggccg ggacgagcga tcagcgccgg tgtcctctct gcaaacctta agcgtgaact
    21061 gctgagtgct gagttcttgg tgggccgaaa ggaactcgtg actcagcact caatccccgg
    21121 cattttttat ctctatgcca cgactggcct tgctcctcac gaccctgatt tggggcgcga
    21181 catttcctgc caccaaagcg gccttggagc aaattccacc gctctcattc ctgcttctgc
    21241 ggtttctgct gggcacgctg atcgtcgccg gatggttcgt gatgatgcga caccgggttc
    21301 atcgggggcg agaggtgttg tatgccagcg gcattgccac gattttcttg tttctcggct
    21361 acgtcctcca gactgtcggg cttcggtata gcaccgcgtc gaattccgct ttcatcacgg
    21421 cgctctatgt tctcttcgtg ccgttaatcc ttcgacgctt cgaagggcgg gtattgcttg
    21481 ccacggcgat tgcgactggg ggattatggc tgttagtgaa gccgagcgca tcggtgaaca
    21541 tcggcgacgt catgacgctg ggctgcgcgc tggcctttgc cggccatatc atctgtctcg
    21601 agcggttcac ccgccggtat gatgccccat ccctcctgct ctggcagatg accgccatga
    21661 gcatcttgtt tattccgacc acgctgtggg aacaggcccc gggggctgcc ttcgctccca
    21721 cggcagttct gttgatcgga ttggctgtga cagggggatt ggcgacagga gcgtttgcgg
    21781 tacagatgtg ggcgcagcag atcgttccgg cgcaacaagt ggccctggtc tttgcctcgg
    21841 agccggccta cgcggcctgg ctctcgtggt attttctcgg tgagacgttg gacctgcaag
    21901 gatggatcgg gagcgggatg atcctgctgg cggtgctcat cggtgcattc agttcaacga
    21961 catctcaggt ggcttccgag accgctcaac cggcgtaacg caggacgaac ggaatctcat
    22021 ggcgcaaaag ttcggtaacg ggcgttgggt gcaagaagga tttctcgaca accgtgtcga
    22081 ggggactgtc gtcggccgca tcgtgttcgc tgcgatcggc ccggtcgacc tgtacctgaa
    22141 gggcaacttt aaggccgaca ttgccggtca ggtgatccag ttccggaaca gccgcttcga
    22201 ggatgacgac atggccgccc aggtgatcgg cgacatggag atcccgcaga tcgggacggt
    22261 caatctcatt tcgttcgatc cgcacccgaa tctggtgccg catccctacg tcgagtggtt
    22321 ctcgaccgga aataaccact atcgatttga gctgaatacc ggtgaggcct ggattgttcc
    22381 ggaaggagag cggagcatga tcgatcggga gagccggaga atccgcgagg ccttcagcgg
    22441 agtttcgtcc cagcggaaca ggtctgagga agattcagac tgggtgtaga gaggcttcgg
    22501 ctgaggggca gagatctcaa tgccagccac ctagccttga gcctcgacct cagccttcgc
    22561 gtgaattaca cagcgattgc ttcgtgtttg ctcttctgct cacgtacata gaccggcacg
    22621 tctttgatgt cccacaccag tccaagccat tccatggtct tcaggcagta ataggtcatg
    22681 tcgatttccc accagtagaa tccctgccgc gtcgaagccg ggtagtaatg gtggttgttg
    22741 tgccagcctt cgcccatcgt gatcatcgcg agaaagaagt tgttgcggct gtcgtctccg
    22801 gtttcgtaac gctgtgagcc gtagacgtgc gagagcgagt taatcgtaca ggtgccatgg
    22861 atcagcgcga cggtcgatat gaagaatccc cagatcagca tctgcggtcc gttcgtgccg
    22921 agttccgggg cgtaggcttc cagcagtttt cccaatccga acatgccgaa ccccgcgatt
    22981 gtcgggacga ggatgtcgaa ccgatcgaga aagcgcagct ccggatactt ggcaaagtcg
    23041 gcgatggctt tcagtctggg cgcaaagtgg gtctgtgacg tgaaccagcc gatgtgcgac
    23101 cagaggaatc ccccttgccg cggcgaatgg acgtcgtcgg gcgtatcgga cgcaatgtgg
    23161 tggtgacggt gatggccggc ccaccagagc gggccacgct gggcacagga tccgccgagg
    23221 acggcgaagg cgaattggca tgcacgggtc gtcttgaacg tacgatgcga aaaataacgg
    23281 tgataccagc cagtgatggc gaacatccta acaaaataga atgccaccgc gaccgtaacg
    23341 gccgtccagc tggtcccgac ccagatgacg cccaagcaca tcgcgtgaac ggcgataatg
    23401 gggatgcaac ggaaccagtc catcttgggg accccgtccg tttttatata ttcgagaccg
    23461 gcccaagagt cgaaccaacg caggaacgtg aaccagaggt tttgagcttt ggtcgggtgc
    23521 agaattcgta cgtcggacat tcacttacct ccaggtcggt ggcggctagt ctcgccccac
    23581 acagagaatc ccactaattt gtatgaggcc acggttcttg taggtggatc acgtggcagg
    23641 tcgaggattg ctgggttagg acgaggaaaa cgtccaaacc ctgtaataac gggagcgcat
    23701 tatacatgaa agccgcctcg aatggtaccg gattttttga gtctctcgtg agcccacatt
    23761 aattgtactg gaatgaggcg gtatgtctat aaacagcctg aatactgcgc gattgaggga
    23821 gtcctcatgt gttgagggac catccagtaa tttgacagct ccgtaaccgc gtctctagaa
    23881 tgcctaaatt atgaaggatc ggttcttgaa agcctgccga agggaggccg tcgattgtac
    23941 ccctgtctgg ttcatgcgtc aggccggccg ctatatggcc gaataccgct cccttcgtgc
    24001 caaacattcg atgttggacc tctgcaagac ccctgaactg gccgcccaag tgacgcttca
    24061 gccgatagac cgatttccgc tcgacgccgc gattattttt gccgacattc tcctgccgct
    24121 cgaagccatg gggttgaatc tggaatttgc ggaaggggag gggccggtga tccataatcc
    24181 ggtgcgtgaa cgggccgatg tggaccgctt aaaggtgatc aacggaagtg aattggacta
    24241 cgtcgcagag gccattcgtc aaacccgcaa ggcgttgaac gggcgcgtcc cgttgatcgg
    24301 gtttgccggt gcgccgttca ccttggccag ctatgcaatt gagggcggcg ggtcgcgcaa
    24361 ctatgtgctg acgaagtcga tgatgtattg cgagccggag gtctggcaca agctgatgga
    24421 caagctggcc cgggtcgtca ccggctacct ccgcaggcag attcatgccg gcgcgcaggc
    24481 gattcaactc ttcgatagct gggtcggctg tttgtcggcc ggcgactatg cggaatatgt
    24541 gatgccgcat gtgcagctga tttttgaagg attgaaacga gaaggcgtgc cgatgatcca
    24601 tttcgggaca ggcacgaccg cgatcttgcg acagatgcgc gaggcgggcg gggatgtgat
    24661 cggcgtggat tggcggattc atctcgatga agcctggacc atgatcggac acgatcgggc
    24721 ggtgcagggg aatctcgatc ctgtcgcgct ctatgccccg ctccatgaaa tcgaacgccg
    24781 cattgaggat attctgcgtc gcgccgccgg ccgtaacggc cacattttca atctgggcca
    24841 cggcatcctg ccgaccactc ctgtcgatca tgttgaagcg gcgatcaaca tgattcacaa
    24901 actcagtcaa cggtaaaccc cgcgagtctc gatggcatca tcgcagaatc ccatagccgt
    24961 gctgctgatg gcgatggggg ggccggattg cctggagaat gtcgagccct tcctgctcga
    25021 tgtgcgaggt ggacggccta cgccgccaga actcgtcgaa gagattcgcg agcgctaccg
    25081 ggcgaccggt ggaaaatcgc ctgcggtggg aattaccaag gatgtggcga aaaaactcga
    25141 agcgcgcttg aacgaaaccg ggaatgagcg ctaccgggtc tacgtcggct tgcggcattg
    25201 gcatcccttt atcaaagaaa cctatgcgga gttgctggcc gatgcgccgg aacagatcat
    25261 cggtttctgc atggctccgc agcagtcgtc cttgagtacg ggtgcctatc gaaagaaagt
    25321 ggaggaagca caagcctccc tgcagagcac ctgtccggtc agctatgtcg ggagttggaa
    25381 ccggcatccc aagctgatcg cagcgattat cgagaatatc aaacaggggc tcctcaaatt
    25441 tcccgctgat gtgcgggccg gcgtgccggt cctttttacc gcgcacagct tgcctgaacg
    25501 gatcgttgcg atgaaggatc cctatcccga agaagtcaca ggcacggtcg aggcggtgac
    25561 tgcgttgttg ggaaatcagc cgacccggtt tgcctatcag agccaggggc gttcaggaga
    25621 aaaatggctg ggtccgacgg tggagtcggc catcgaggat ctgagccggg caggccatcg
    25681 ccatgtgctg gtcgcgccga tcggtttcct gtgcgatcat gtcgaaacgc tctatgatat
    25741 cgatattgaa ctcaaacagc tggcagccag tcacgggatg cagcttgagc gcattcagat
    25801 gctgaatgac tccccggcct tggtcgaggt gctggccgat gttctggccg cgcacaaatc
    25861 ttccctgacg atcacgtcgt gagcaatccc tgcacagtcg ccatcgtcgg tggaggcatt
    25921 tccggccttt cgacggcgtt tgccttgcag gaacgcgcgg cggcagccgg tcttgcgatc
    25981 cgctgcaccg tcgtcgatgc cgctccttcc tggggcggca aaatcgtcac gcatcgcatc
    26041 ggcgatctgg tgacagaagc aggccctgat tcgttcctgt cgcagaagcc ggccgggttg
    26101 caactggtcg agaaacttgg tcttgccgat caactgatca acacgaacga gacaggcaag
    26161 aaggcctttg tctattcagg cggccgcttg tgcgagttgc ctgaaggtct cgtggtcatc
    26221 acccccggcc agatcgggcc ctttctcaag agtggtctgc tgacggtgag cggcctcgcc
    26281 cgcatgggat tggatctcgt gatgccggcc aagcgctcgc aggaggacga atcgctggcg
    26341 tcgtttttcc ggcggcggtt cggccggcag gccttcgaac gcatgatgga acccttgatg
    26401 gccggcatct atgcaggaga tgcggagcag atgagtgtga acgcgacatt cccccgtttt
    26461 cccgaactgg agcgagagta tgggagtgtc atccgcggga tgatggcggc gaggaaaacc
    26521 ggacggccgc agggatctgc cacgccgcga cggaccatgt tcgtgagcct caggaacggc
    26581 ttggaagatc tcgtcgatgt gctggtccgc cgattaactg agcagagcgt cacgctcaga
    26641 agcggcgctg ttgtcgagtc gttgcgcgta cggtctcacc agttgggacg ctggacatac
    26701 gatgtcatgc tcagcgacgg atcggccctc tcggtggaca gcctggtgtt ggcgacgccg
    26761 gcttacgtct cgagcgaact gttgcgccct ctgacgccga ttgcgggcgg gctgttagat
    26821 atgattccct acgcctcgac ggcaaccatc gccatggcat acccggattc tgtcatcggg
    26881 ggtggggtgc agggatttgg atttgttgtt ccacgcgctg agcaacggga tctgatcgcc
    26941 gcaacctgga cgtcactcaa gtggccgcat cgggcaccat ctgatcggct tctggtccgt
    27001 tgttatgtgg gcggggtcgg gcgagaagcg attctccagc tcgacgatga agcgctcgtg
    27061 gcgcgagtga agtcggagct caagctgatc tgcggcgtga ccgccgaacc gacatatgtc
    27121 gaagtgaatc ggtggatgaa ggcgatgccg cagtatctgc tgggccatct tgaacggttg
    27181 gaccaggttg atgcggcatt gagccgctac ggcggattgg tgctgaccgg cgctgcctat
    27241 cgcggggtag ggattccgga ttgcatccgt gacggggcgg ttgcggccga ccgggtgatc
    27301 cgttatcttt caggcgagcg ggtttagcgg attcaaagga aagtgggaat gggaacagaa
    27361 actaagatga cgattgttga tgtaagcgga ccgtttcgtg aaccgcgaga gctggcgttt
    27421 tcctatgact actccattca acggatgaat tggccgactc cccatgccgt gcgcgtaaag
    27481 gtgggaatcc cagaagagtt ggataccctg agggcgaaaa ttctcggtgc aacgaccgga
    27541 acgccggggc agcaactggt catttcccag atcatgtcca ggcggattgc ggacgagaaa
    27601 ctccgcatcg ccgaggggga gggacagctg ctggaacggc gtgaagtggt catcccgccc
    27661 tatgccgggc cgctcgcaca catgtatgcg cgtctcgaat catgggtgga acgcgaacgg
    27721 gagacgttgc gggcggagat catgaagcgg gcgaatctct agtaggctgt tgaaaaagcc
    27781 cggcagtatc actagaaaat ttttcgataa tgcttcgaat ggacgcccg
//
LOCUS       DATIFR010000210         5562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1484230, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000210 DATIFR010000000
VERSION     DATIFR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484230"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VLD60_12160"
     CDS             complement(<1..518)
                     /locus_tag="VLD60_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSB45762.1"
                     /translation="MSLHKLSLCELQKKFTAGEVTATEIVRAYFLRIGQVESKVKAYV
                     TQVKEAALVQAGALDNKLKGWRRTKPMTGMPLAIKDNLCTEGVLTTCSSRMLQNFVPP
                     YDATVIAKLREQDYILLGKTNLDEFAMGSSTENSAFGPSRNPWNFHCVPGGSSGGSAA
                     AVAADECVAALGS"
     gene            complement(518..883)
                     /gene="panD"
                     /locus_tag="VLD60_12165"
     CDS             complement(518..883)
                     /gene="panD"
                     /locus_tag="VLD60_12165"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HSB45763.1"
                     /translation="MFRQMLRSKIHRATVTGAHLEYEGSLTIDQDLMEAAAILPYEAI
                     ICSNLNNGERFMTYAIGGRRGSGDIVLNGPTARKAAVGDQIIIFCYEYYGEEEIKRHA
                     PKIVRVNDKNRIVESTDKR"
     gene            complement(980..1267)
                     /gene="gatC"
                     /locus_tag="VLD60_12170"
     CDS             complement(980..1267)
                     /gene="gatC"
                     /locus_tag="VLD60_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSB45764.1"
                     /translation="MEITRQEVEKVAKLARLEITSAEKEAFAKQLSLILTHVEKLKQY
                     DTTGVEPTSTVFGQVNVFRDDVTRASLPVEKALANAPEREADGFCVPKIIE"
     gene            complement(1352..3181)
                     /gene="glmS"
                     /locus_tag="VLD60_12175"
     CDS             complement(1352..3181)
                     /gene="glmS"
                     /locus_tag="VLD60_12175"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSB45765.1"
                     /translation="MCGIVGYVGNQEAVPILIGGLAKLEYRGYDSAGVAVQNGEKIEV
                     RRSVGKLINLQKSLDEKALKGVVGIGHTRWATHGKPSEQNAHPHRSKGCVLVHNGIIE
                     NYQPLKEQLRREGYKFVSETDTEVVAHLIDKHLQKGKKLADAVRAATKDVRGSYALAV
                     ISEREPGTLVAARSGCPLVIGRTKHESFVASDVMAMLAHTRDVTYLEEGDVAVVTSSD
                     VQLTDAEGRTITRKPTKITWDASAAEKSGFPHFMLKEIHEQPQTILDTMRGRYSYETG
                     EADLPDIGLSPQQFAAVGRIWIVACGTSWHAGKVGKYLLEEMVRTPVQVDIGSEFRYR
                     DPLVEKGDLFITISQSGETADTLAAAREARGKGAHVVSIVNVVGSTLARESDGVLYTH
                     CGPEIGVASTKAFTAQLTALYLLALHLARVRNVMNVADGKAWLDRLVRLPVLVERILG
                     REAEIVAIARRYYKKRNFLFLGRGINYPIALEGALKLKEVSYIHAEGYAAGEMKHGPI
                     ALIDKDMPVVVVAPKDRLYEKTVSNLMEVKARRAPVIALVAEGERELGKIADAIFTIP
                     DTHPLISPILFTIPLQLLAYHIAVLRGADVDQPRNLAKSVTVE"
     gene            complement(3184..4788)
                     /gene="glmU"
                     /locus_tag="VLD60_12180"
     CDS             complement(3184..4788)
                     /gene="glmU"
                     /locus_tag="VLD60_12180"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HSB45766.1"
                     /translation="MSRLKEHTSVMSRMPLEGSPAGTVAGLAVVVMAAGLGKRMQSKH
                     AKVLQPVAGRAMVLYSVELGLRVAGHRVVVVVGHQADQVRQVVDAATSGSNGHAPVTI
                     VEQREQLGTGHAVLQSRPVFTESGRPAPSTYLILNGDTPLLREATVRELLRVHQAENA
                     AVTILTAILDDASGYGRVVRQSSAGSQVLRIVEDRDANKHEQSIREVNVGTYVVEGDF
                     LFSALEKLQPSNAQGEYYLTDIVQMAVEQGRPGAVVVLNHPNEGLGVNTQRQLAEAEQ
                     VVRWQIRHRWLDAGVRMIDPSSTWIDAGVTIGRDSVLYPNVILEGATVIGEDCVVRSG
                     TRITNCVVGDRVEILDHCIFRDSRIESDTTLGPFTHLRPQVLVRKGAKVGNFVEAKKT
                     ELGEGSKANHLSYLGDAKIGKGVNIGAGTITCNYDGVRKAETVIGDGVFVGSDTQFVA
                     PVTVGSGSVIAAGTTVTENVPADSLVIARVPQVTRPGWAARRRAFQTDAKRETTSTRL
                     KPRASRVTKKQPLASKGRQVKKRNGG"
     gene            complement(4785..5453)
                     /locus_tag="VLD60_12185"
     CDS             complement(4785..5453)
                     /locus_tag="VLD60_12185"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSB45767.1"
                     /translation="MLKASLKRYFLTGLLVVTPIWGTFLILKTLFVAVDGILGDLLAQ
                     LVPDHYVPGLGIVTLILLVFVTGLFAANFMGRQIVKLWEGWLNRVPLVRGIYSTLKSM
                     MDILSFSERGSYHRVVLIQFPKNGHYCFAFVTGVTKGDLQDLAQGPLLHVYVPTSPNP
                     TSGYFLLVPEGEVSSVNISVEEAMKLIVSGGLYSPTPPLGSAVGPEPKWSAVKQPEAG
                     VPIG"
BASE COUNT         1186 a         1733 c         1378 g         1265 t
ORIGIN      
        1 gaccccaggg ctgccacgca ctcgtccgcc gccaccgccg cggcagatcc tccgctcgag
       61 cctccgggca cacagtggaa gttccaggga tttcggctgg gaccgaaggc agaattctcg
      121 gtcgaggatc ccatggcaaa ctcgtccagg ttcgtcttgc ccaacagaat gtaatcctgc
      181 tcgcgcaact ttgcgatgac cgtcgcgtca tagggaggca caaaattctg aagcattcgg
      241 gaagagcagg tggtaagcac tccctccgtg cagagattgt ccttgatcgc cagcggcatc
      301 ccagtcatcg gtttcgtcct gcgccagccc ttcagtttgt tgtccaacgc accggcctgg
      361 accaatgcgg cttctttgac ctgcgtcaca taggccttaa ccttcgattc cacctggccg
      421 atacgcagaa agtaggcgcg tacgatctcc gtggccgtga cttcgcccgc ggtgaatttc
      481 ttctgcagct cgcacagtga cagtttatga agcgacatta ccgtttatcc gtcgattcaa
      541 cgatgcgatt tttgtcgttc actcggacaa tcttgggcgc atgccgcttg atctcctctt
      601 cgccgtagta ctcgtagcaa aagatgatga tctggtcacc gaccgcggct ttcctggctg
      661 tcggcccgtt caggacaata tcgccgctcc cccgccggcc tccgatggca taggtcatga
      721 accgctcgcc gttgttgagg ttcgagcaaa tgatcgcttc gtacggcaaa atcgccgccg
      781 cctccatcag atcctgatcg atagtgagac tgccttcata ctcgagatgc gcccccgtta
      841 ccgtggcgcg atggattttc gaccgcaaca tttgtcgaaa catgtattcc cctcgtgaaa
      901 atcaaaacgt gcaccgtgaa aggtttcgtc tatctccggg tcgggcacaa ctttcagatg
      961 tcacatttca cggttgtcgt tattcaataa ttttcggcac gcaaaaccca tccgcttccc
     1021 gctccggcgc attggccaag gccttctcca ccggaagcga cgcacgcgtc acatcatccc
     1081 ggaatacatt cacctgcccg aacaccgtcg atgtcggctc gacaccagtc gtgtcatact
     1141 gcttgagttt ttccacatgg gtcaggatca gactgagttg cttggcaaac gcctccttct
     1201 cagccgaagt gatctccagc ctcgccaact tggcgacttt ttccacttct tgtctcgtga
     1261 tttccatgcg atggttcctc tggtttgttt ggtttgtttt gtttatctgg ttcaaccaaa
     1321 ggaacaagac aaacgagaca aacctccctg actattccac cgtcacactc ttcgccaaat
     1381 ttctcggttg atcgacatcc gccccacgca gaactgcaat atggtaagcc agcaactgca
     1441 aggggatcgt gaagagtatc ggcgaaataa gcgggtgtgt gtccggaatt gtaaaaatcg
     1501 catctgcgat cttacccaac tcccgttctc cttccgctac caatgcaatg accggtgcac
     1561 gtcgtgcctt gacttccatc agattgctga ccgttttttc atagagtcga tccttcggcg
     1621 ccaccacgac gaccggcatg tctttgtcga taagggcaat cgggccatgc ttcatctcgc
     1681 cagcggcata gccttcggca tggatatagg acacttcttt cagtttcagt gcaccttcca
     1741 gcgcgatggg ataattgatg ccccgcccaa gaaataggaa gttgcgcttc ttgtaatacc
     1801 gcctcgcgat cgccacaatc tccgcttcgc gtcccaggat gcgctccacc aacacgggca
     1861 ggcgaaccaa gcgatccaac caggccttgc cgtccgccac gttcatcaca ttacggacac
     1921 gcgcaagatg cagcgcgagc agatagagcg cggtcaactg cgccgtaaag gctttggtcg
     1981 aagccacgcc gatttccgga ccgcaatgcg tatagagcac cccgtccgat tcacgagcca
     2041 gcgtactccc gaccacattg acgatcgaca cgacgtgagc cccttttccc ctcgcttcac
     2101 gtgcagcggc gagcgtgtcg gctgtttcac cggactgcga aatagtgatg aacaaatcgc
     2161 ccttctcgac aagggggtcg cgatatcgaa attcgctgcc gatatcaacc tggaccgggg
     2221 tccttaccat ttcttccaag agatactttc ccaccttccc cgcatgccag gaggtgccgc
     2281 aggcgacgat ccagatccgc cccactgccg caaactgttg cggcgaaaga ccgatatcgg
     2341 gcaaatccgc ctcccccgtt tcatacgagt accgcccgcg catcgtgtcg aggattgtct
     2401 gcggctgctc gtgaatttcc ttgagcatga aatggggaaa cccgctcttc tcagccgctg
     2461 aggcgtccca ggtgatcttc gtgggcttcc gcgtgatggt ccgcccttca gcatccgtca
     2521 gttggacgtc cgaagatgtg acgactgcca cgtcaccctc ttccaaatac gtgacgtcgc
     2581 gagtatgggc cagcatggcc atcacatcgg acgcaacaaa tgactcgtgc ttggttcgac
     2641 caatgacgag cggacagccg gaacgagccg ccactaacgt cccaggttct cgctcggaaa
     2701 taacggcgag cgcgtaactg ccgcggacat cttttgtggc cgcgcgcact gcgtcggcca
     2761 gtttcttccc tttctgcaaa tgcttgtcga tgagatgggc cacaacttcc gtgtcggtct
     2821 ccgagacaaa cttgtatcct tcccgccgta attgctcttt caagggctga taattttcga
     2881 tgatgccgtt gtgcaccagg acacatccct tggaacgatg cggatgcgca ttctgttccg
     2941 atggtttgcc gtgagtcgcc cacctcgtat ggccaattcc gacaacgccc ttgagagctt
     3001 tttcgtcgag tgacttctgg agattaatca gcttgccgac actgcgccgc acctctattt
     3061 tctctccatt ctgcacagcc acgcccgcag agtcgtaccc tcgatactca agcttcgcca
     3121 ggccgccgat gagaatcgga accgcttctt gattgccgac atagccgacg atgccgcaca
     3181 tacttaaccc ccgttgcgtt tctttacttg tctacccttc gaagccagcg gttgtttctt
     3241 cgtcacgcgt gaggcgcgag gcttgaggcg agtggatgtc gtttcacgtt tcgcgtcggt
     3301 ttgaaatgcc cgccgccttg ccgcccatcc tggtcgagtc acttgaggta cccgtgcaat
     3361 cacaagagaa tcggccggaa cattctccgt taccgtcgtc ccggccgcga tgaccgaacc
     3421 agaaccgacc gttaccggcg cgacgaattg cgtatcgctt ccgacaaaca ccccatcgcc
     3481 aatgaccgtt tcggctttcc gtacgccgtc gtaattacac gtgatcgtcc cagcgccgat
     3541 attgaccccc ttgccgatct tcgcatcgcc gagatagctc aagtgattgg ccttcgaccc
     3601 ttcgcccagt tcggtttttt tcgcttcgac aaaattgccg acctttgccc ccttccgcac
     3661 caacacttgc ggccggagat gcgtaaaggg gcccagcgtc gtatcgcttt cgattcgcga
     3721 gtcacgaaag atgcaatgat ctagaatctc gactcgatct cccaccacgc aattggtgat
     3781 tcgggtccca gaccgcacca cgcaatcttc tccaatgact gttgcacctt cgaggatcac
     3841 attggggtac aggacggaat ctcttccaat tgtgaccccc gcatcgatcc acgtcgaaga
     3901 gggatcaatc attctcactc ccgcatcgag ccaacgatgg cgaatctgcc agcggacgac
     3961 ttgctcggct tcagcaagct gtctctgcgt attcacgccc aaaccttcgt tcggatgatt
     4021 cagaacgacg acggcaccgg ggcgaccttg ctccaccgcc atctgcacga tgtcggtcag
     4081 gtagtactct ccctgcgcgt tgctcggctg gagtttctcc aacgccgaaa agagaaagtc
     4141 cccttcaacc acgtaggtcc cgacattgac ttcgcgaatc gactgttcat gtttattggc
     4201 atcgcgatcc tcaacgattc gcaacacctg actccccgcc gatgattggc gaacgactct
     4261 gccgtaccca ctcgcatcat ccagaatcgc cgtgagaatg gtcaccgccg cgttctccgc
     4321 ctgatgcaca cgcaacagtt cccgcaccgt cgcctcgcga agtaacggcg tatcgccgtt
     4381 gagaattaga tatgtcgacg gcgcgggacg tccggactct gtaaagacgg gacggctctg
     4441 caggacagca tggccggtcc ccaattgttc ccgttgctca acaatcgtga cgggcgcatg
     4501 gccgttgctt ccagatgtcg ccgcatcgac gacctgccga acctgatcgg cctgatgacc
     4561 gacgacgacc actacccgat ggcctgctac gcgcaagccc agttcaacgg aatacagcac
     4621 catcgctcgc cctgccaccg gttgcagcac tttcgcatgc ttggactgca tccgtttacc
     4681 caatccggct gccattacga cgactgccag tccagcaact gttcctgcag gactcccttc
     4741 cagcggcatc cggctcatga cagaggtatg ttctttcaat cggctcatcc gatcggcact
     4801 ccggcctctg gttgcttgac ggcactccac ttaggttcgg gccccacagc cgacccaagc
     4861 ggcggcgtcg gtgagtagag tccgccggac acgatcaact tcattgcttc ctccacactg
     4921 atattgacgg agctcacttc accttccggt acaaggagga aataaccaga ggtcggattg
     4981 ggcgaggtcg ggacatagac gtgaaggagc ggcccctgtg ccaaatcctg caagtctcct
     5041 tttgtcacac ctgtcacgaa ggcgaaacaa tagtgcccat tcttgggaaa ctggatcagg
     5101 acgacgcggt ggtatgatcc gcgctcggag aacgacagga tgtccatcat ggatttcagc
     5161 gtcgagtaaa tgcctcggac cagcggcact cgattgagcc acccttccca caacttcacg
     5221 atctgcctac ccatgaaatt cgccgcgaag agtccggtca caaacactaa caagatcaag
     5281 gtgacaattc ccaacccggg cacatagtga tcgggaacca gttgcgccaa aagatctcca
     5341 agaataccgt ccacagcgac gaagagagtc tttaagatca gaaaggtgcc ccagatcggc
     5401 gtcacgacaa ggagaccagt cagaaagtag cgttttagtg aggctttcag cataaatata
     5461 ggtggacaga aatagcggct catcatacag agatttacgt ggctgtcgca agcattttct
     5521 tgctattttc aacaacttgg aatactatcc cgcgcgtttt tt
//
LOCUS       DATIFR010000211        11301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2442158, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000211 DATIFR010000000
VERSION     DATIFR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442158"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VLD60_12190"
     CDS             complement(<1..1171)
                     /locus_tag="VLD60_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45768.1"
                     /translation="MRSSPAECLTGMLNRLFSWIDRNVLSLGRDMRLSYLPPLMVYMA
                     YGISGLTGIVGTFFVKEYLGLSASFLAALGFWAGIPWALKMPIGHTVDLLWRWKSWLV
                     GLGAMLLAASLGIMAALIGNREAMTAIMPAEVWYVLSVLLAPIGYVIQDAVADAMTVE
                     AVPRVDERGQPFDEPTRKLMHTTMQTLGRVAIVGGGIIVAMINVYVFTGVEGLPQAEI
                     VRLYKQVYLMALIIPFVSVLGVGIAWGLRRRHSRQLLQQGFSQAQAEQMVTTRDEARE
                     PTTPNWWILGGSLAFVLFTLTVGFAGFAYSEEIVFAGSMAIVLFLMARLVRELEPDKR
                     FTLVGTAVVIFIFRAIPGTGPGTTWWMIDQLKFDQQFLSVLSLIGSTLTLAGMFVF"
     gene            complement(1152..1538)
                     /locus_tag="VLD60_12195"
     CDS             complement(1152..1538)
                     /locus_tag="VLD60_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB45769.1"
                     /translation="MARQNISTGGPWEDKIGYSRAVRTGSSVYVSGSTAMTPSGLVGK
                     GDPYAQTIQTFKTIEAALKQAGASLNDVVRTRIYMANIDQWQEVGRAHGEIFGNIRPA
                     TTMVEVNRLIDPDMLVEIEADAIISG"
     gene            complement(1563..2513)
                     /locus_tag="VLD60_12200"
     CDS             complement(1563..2513)
                     /locus_tag="VLD60_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HSB45770.1"
                     /translation="MNEKKIIAVVGATGAQGGGLVRAIMSDPGSGFIARALTRDVKAD
                     KAKALAKLGAEVVAANLDDVESLKRAFAGAYGVFCLTNFWEHFSPEKEYAQVKAQAEA
                     AKQASVQHVIWSTLEDTRQWVPLSDTSMPTLMGKYKVPHFDAKGEADQEFTKLGVPTT
                     FLLTSFYWDNMIFFGMGPKKGPDGVLAFTLPMGDKKLPGIAAEDIGKCALGIFKKSRE
                     YIGKRIGIAGEHLTGAEMASAMSKAFGQPVRYNDVSPEVYRGLGFPGADDLGNMFQFK
                     HDFNDVFCGPRNPAVARGLNPALQTFEQWLAEHKNRIPLT"
     gene            complement(2766..3497)
                     /locus_tag="VLD60_12205"
     CDS             complement(2766..3497)
                     /locus_tag="VLD60_12205"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB45771.1"
                     /translation="MASLSGKVAIVTGSSSGIGRAIAERLAEQGAIVVVNYSKSHEKA
                     QQVVMGIQGEGDRAIAMQADMSQVADARGLVAETMKHFARLDILVNNAGKFMPKLLAD
                     MTEAEFDHVFALHAKGPYFAMQEAAKVMKDGGRIVNISTSGTQRVFHGATAHLGSKAA
                     LEQYTKGLALELAPRNITVNTVSPGITDTGVLTEQYRQFGLQVSPFKRLGTPKDVADV
                     VAFLVSEEARWLTGQNIQAGGGMVM"
     gene            complement(3621..4499)
                     /locus_tag="VLD60_12210"
     CDS             complement(3621..4499)
                     /locus_tag="VLD60_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB45772.1"
                     /translation="MADRKKRLDANVAGNFYVDSTCINCDTCRQLAPNSFEEIGEYSA
                     VTIQPQGDRQVQQAYQALLACPVGSIGTEQGDRARVQAAMASFPLRLDENVYYCGFNS
                     EKSFGANSYFVQHPEGNWLIDSPRYIKHLVEMFERRGGIRNIFLTHEDDVADAAMYAK
                     RFGARRIIHRADAHAMPDAEWIVDATEPVLLAPEFHMIPVPGHTPGSSVLLHQSRFLF
                     SGDHLWWESDRRQLGAPRELVWNHDELMRSIEKLLDYRFEWILAGHGDRTHLVQEQLR
                     TKVQELLERRRSSVRT"
     gene            complement(4511..5110)
                     /locus_tag="VLD60_12215"
     CDS             complement(4511..5110)
                     /locus_tag="VLD60_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HSB45773.1"
                     /translation="MRRLIHEIGRFTLMQRVGRSPFESLARAIAYQQLHDNAAESILR
                     RFVALFPGRRFPRPAEVLGANVRAMRRAGFSRAKVAALRDLATKALEGTVLTTRVITQ
                     LTDEAIIERLIEVRGVGRWTVEMLLIFQLGRPDVLPVHDFGVRNGFRIAYGRRSMPTP
                     KEVLHYGERWKPYRTAASWYLWRAVDRSKNGKSAPGVTV"
     gene            complement(5165..5767)
                     /locus_tag="VLD60_12220"
     CDS             complement(5165..5767)
                     /locus_tag="VLD60_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSB45774.1"
                     /translation="MEKPATTASPIHELIARRWSPRAFSDQPVEAVKLRSLFEAARWA
                     PSSNNEQPWRFLVAIKEQEEDFARLLACLLEGNRKWAYRAPALILSVASLNFEENGKP
                     NRHAFHDTGMAVENLVLQATALGLFAHQMAGFDVEKARADCSIPLGFDPVAMIAVGYP
                     GDPAVLPDYLREREVKPRDRQPADDFVFSAHWGRRSPLIG"
     gene            complement(5776..7191)
                     /locus_tag="VLD60_12225"
     CDS             complement(5776..7191)
                     /locus_tag="VLD60_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSB45775.1"
                     /translation="MRGLCLVALSVLLVSCAMGPDYSRPDIPTSDSFRMAGEGKDLPS
                     LANAPWWELYHDEELQKLIRIALEENKDLKRSVATVDEFAARALIAKTDFAPQMNATV
                     NAPTFGNTKNIAFPGFPNPFNYYAQGNLAWELDIWGRIRRSNEAALGDLLAREENRRA
                     LIVQIVGGVAQGYFDLRQFDMQLDIAMRTLQAWEESVRIGQARLRQGLTNRLDVDQFE
                     AERENAAARIAELKRQMVQKENELSVLLGRNPAQISRGRSLTEQVMPPVVPAGLPSEL
                     LQRRPDVVQAEQGLAAATARIGMAKADRFPKLSITGILGVASPQLTQLVANETFFGVA
                     GPSLTGPLFNAQILGFQQKAAEAQARQALAQYEQTILVAFREVDDALVGVSTAREQAA
                     AQERQVNALRSALRLANLRYQGGLANYLDVLIAQRSLFDAELALAATHRLHLVSVVQL
                     YKALGGGWSPDKDQVKDDGKG"
     gene            complement(7193..10381)
                     /locus_tag="VLD60_12230"
     CDS             complement(7193..10381)
                     /locus_tag="VLD60_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HSB45776.1"
                     /translation="MISHFFIDRPIFASVLSIIIVVVGLVSLNALPIAQFPEITPPVV
                     QIEADYPGASAEVVADSVARTIEVQLPGIDNLLYYDSTSTNDGHMTIRLTFEIGTDVD
                     IAQVQTQNREKLAEPQIPDEVIRQGISVKKVSPDLLAVIALSSTDPRHDTVFLSNYAI
                     LRVIDNLKRLKGVGDAYVFGQQNYSMRLILDPVRMAQLSLTPTDIANVVREQNRDFPA
                     GTIGREPATKGTELTIPVMTLGRLTDVKDFEEMIVRALPNGSMIRLKDIAKVELGAQS
                     YTLEGRWNGKPNVFLLTFLSPGANALDSVKRIRKEMGEVAKSFPPGVSWDVPYDTTRF
                     IEVSIEEVVKTLGEAMILVVLVVYLFLQSWRTTLIPGVAVPVSLIGTFAGMQALGFSI
                     NTLTLFGMILAIGLVVDDAIIVVENVERHMEEERCSSKEAAKRAMTEVTGPVIAIVLV
                     LCAVFVPVGFLGGITGQLYKQFAITIAMSVTISGFVALTLSPALCALVLKPGGKGQRT
                     GFFALFNRLFAWTQNRYTASVAGALKRSGLVLAVFGVLMVFVFGLFRVIPSSFLPEED
                     QGYFITMFQLPDGASKQRTDAVLSKVEHYFLANSAIHSTDAMSGLNFIFNTRGPNTAT
                     MFVPLHLWNQRKEPHQHVKALIGAAYGEFAKIPEALILAFNAPSIRGLGATGGFSLQL
                     QDPSGGDFKKFAAVVQDFLAKARKDPAIGAIGTSFRVSSPRLYAHVNRERAKALGVPI
                     AEVFDTLQAFFGNLYVNDFLKFGRVYRVQTEAEAQYRSKPEDISKIYVRALSGQGPSM
                     IPLDTVVTTEYTSGPDPVTHFNGFNTALVLGTAAPGYSSGQVLDALDRVAKEVLIPQG
                     YGADWSGISYQERLVGRQSIYAFAFGLLMVFLVLAAQYESWTVPFAVILAVPFGVFGA
                     LLAVWLRGLSNDIYFQIGLVTLIGLAAKNAILIVEFAKHRHEAGMSPREAAVEAAKLR
                     FRPIIMTSMAFILGVLPLVVATGAGAASRQSIGTGVFGGMLAATFLAIFFVPLFFVLV
                     RKFSRGSASHALVVGTGEERAIPPQSSAEGEH"
     gene            complement(10378..>11301)
                     /locus_tag="VLD60_12235"
     CDS             complement(10378..>11301)
                     /locus_tag="VLD60_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB45777.1"
                     /translation="DRLYQIDPVPFKAALLSAKAKVAQAEARLVQAKQNLARVKPLLA
                     EQAVSQKDVDDGVAEELAAAAALDGAKADLVKAKFDFDNTFIIAPINGMIERTRVYEG
                     RLVSAQTDLLTIVHQIDPMYVTVSAPESFLLKRRRDIAAKRIEHPGVYQLRGTIIFLD
                     STTYPHEGVLDLLDVGMRTETGSRPARVTFPNPDQILVPGQFVTVRFKGTVKTGAILV
                     PQRAVQQGPKGPVLFVVLADGKVEVRDVQATSWQGNQWLIEEGLQAGELVVVDGFQRI
                     VPGAPVKPVSSSVAEATPVDQPAATKTEPTK"
BASE COUNT         2311 a         3469 c         3221 g         2300 t
ORIGIN      
        1 gaaacacaaa catgccggcc agggtgagcg tgctcccgat caaggacaag accgagagaa
       61 attgttggtc gaacttgagc tggtcgatca tccaccaggt cgtcccaggc ccggtccccg
      121 ggattgcacg gaaaatgaag atgacgaccg cggtcccgac gagggtgaat cgcttgtcgg
      181 gttccagctc acgtacgagc cttgccatca aaaacaagac gatcgccatt gaaccagcga
      241 agacgatttc ctcgctatag gcaaagccgg caaacccgac ggtcagcgtg aacaacacga
      301 aggcaaggct cccgccgaga atccaccaat tgggcgtcgt cggctcacga gcctcgtcac
      361 gcgttgttac catttgctcg gcctgtgctt gcgaaaaccc ctgttgaaga agctgtcgac
      421 tgtgccgtct ccgcaggccc cacgctatcc caacgccgag aaccgagacg aacgggatga
      481 tgagcgccat caggtagact tgtttgtaga gacggacgat ctccgcctgc ggaagtcctt
      541 cgacgcccgt aaacacatag acgttgatca tcgcgacgat aattccacca ccgacaatcg
      601 caacacggcc cagcgtctgc atggtggtgt gcatgagttt ccgtgtcggt tcatcgaacg
      661 gctgcccgcg ctcatcgaca cgagggacgg cctcgaccgt catcgcgtct gccaccgcat
      721 cctgaatcac atagccgatc ggcgcgagaa gaacgctcaa gacgtaccag acttccgccg
      781 gcatgatcgc ggtcatggcc tcgcgattgc ctatcaacgc cgccatgatg ccgaggctcg
      841 cggcgagcag catggcgcca agccccacga gccagctttt ccagcgccac aggagatcga
      901 ccgtatggcc gatgggcatc ttcagtgccc agggaatgcc ggcccagaat ccgagcgcgg
      961 cgaggaagga tgcggagagg cccaaatatt ctttgacgaa gaacgtgccg acgataccgg
     1021 taaggccgga gatgccgtag gccatgtaga ccatgagcgg cggcaggtag gacaaccgca
     1081 tgtcccggcc cagcgagagg acgttgcgat cgatccagct gaacagtcgg ttgagcatgc
     1141 ccgtgagaca ctcagccgga gatgatcgca tccgcctcga tttcgaccag catgtccgga
     1201 tcaatcaagc gattgacttc gaccatggtt gtggcgggcc ggatgtttcc gaagatttca
     1261 ccgtgcgccc ggcctacctc ctgccactga tcgatgttgg ccatgtagat gcgtgtccga
     1321 acgacgtcgt taagcgacgc ccctgcctgc ttcagcgcgg cttcaatggt cttgaaggtc
     1381 tgtatggtct gcgcgtaggg atcgcccttg ccgaccagcc cggaaggcgt cattgccgtc
     1441 gagcccgata cgtatacaga tgaaccggtg cgcaccgcgc gtgaatagcc gatcttgtct
     1501 tcccagggac ctccggtcga aatattctgt cgtgccatcg tcactccttt gtcatctcat
     1561 cgttaagtga ggggaatccg attcttatgc tcagcgagcc actgctcaaa ggtttgcagg
     1621 gcaggattca ggccgcgcgc cacagcggga ttgcgcggac cgcagaacac gtcattgaaa
     1681 tcgtgtttga actgaaacat gttgccgaga tcatcggcgc cgggaaaacc aaggccccga
     1741 tagacctccg gcgacacgtc gttgtaccgc acgggctgcc cgaatgcctt gctcatcgcc
     1801 gaggccatct ctgcaccggt cagatgttcg ccggcaatcc cgatccgttt tccgatgtac
     1861 tcacggctct tcttgaagat ccccagcgca catttgccga tatcctcagc ggcaataccc
     1921 ggcagtttct tgtcgcccat cggcagggta aacgccaaca cgccgtccgg accctttttc
     1981 ggccccatcc cgaagaaaat catgttgtcc caatagaatg aggtcaggag aaacgtcgtc
     2041 ggaacgccca gcttggtaaa ctcctgatcg gcttcgccct tggcatcaaa atggggaacc
     2101 ttgtacttcc ccatcagcgt tggcatggag gtatccgaga gcggaaccca ttgtctcgta
     2161 tcctccagcg tcgaccagat cacatgctgc acggatgcct gtttcgcggc ttccgcctgg
     2221 gccttcacct gggcatattc tttctccggc gagaaatgct cccaaaagtt cgtcagacag
     2281 aacacgccgt aggcgccggc gaaggcgcgc ttcaggcttt cgacatcgtc gagattggcc
     2341 gccaccacct ccgctcccag cttggccagg gctttggctt tgtcagcctt cacatctctg
     2401 gtcaacgcgc gagcaataaa gccgctgccg ggatcgctca tgatcgcccg gacgagcccg
     2461 ccgccttgcg cgccggttgc accgaccacc gcaataattt tcttttcatt catgatcttc
     2521 ctccctaccc ggatgttcaa aaggaccact gttcacaccc gcccaacccc ggcgcgccac
     2581 ggcgcgccct tccgcgggaa ggccgcagcg agcgaggcgg cgaggaggta cataccaagc
     2641 ttcgcttgaa ccgctcgctt cgatcacatg cgagcggata gttactttgc ctcaaggaag
     2701 ctccttagta cgttatgcct ctgatcgacg tgagaacgcc gctggcggtc tttttcaaca
     2761 tcctgtcaca tgaccatgcc gccgccggcc tggatattct gccctgtgag ccaccgcgcc
     2821 tcctcactca caaggaaggc caccacatcg gcaacgtctt ttggtgtccc gagtcgtttg
     2881 aacggcgaca cctgtaaccc gaattgccga tactgctccg tcaacacccc tgtgtccgtt
     2941 atccccggtg agacggtatt gaccgtaatg tttcgcggag cgagctccag cgccaaaccc
     3001 ttcgtgtact gttccagcgc ggccttgctg ccgagatgcg ccgtcgcccc atgaaacacc
     3061 ctctgcgtgc cggaggtcga aatattcacg atccggccgc cgtccttcat gacctttgcg
     3121 gcctcttgca tggcaaagta tggtcccttg gcgtgtaacg cgaacacgtg gtcgaattcc
     3181 gcttcggtca tgtctgccag cagcttcggc atgaatttcc cggcattgtt caccagaata
     3241 tccaaccgcg cgaagtgctt catcgtttcg gccacgagtc cacgcgcatc ggccacctgg
     3301 ctcatgtcgg cttgcatggc gatggctctg tcgccctcgc cctgaatacc cattaccact
     3361 tgctgcgctt tctcatggct cttggaataa ttcacgacga cgattgcgcc ttgttccgcc
     3421 aaccgttccg cgatcgcccg gccgatcccg ctggacgacc ccgtgacgat cgcaaccttt
     3481 ccgctgagtg aggccataga actcctcctt ctgtcgggtt tcctccagca ggtcgatcgt
     3541 gtcggtatcg acgaccgctt cgatcaatca cagccagccg agttcacgat attctgcatt
     3601 gctgtgaaag atgtattcgc tcatgtccgc acggaagaac gtcgccgttc aagaagctct
     3661 tggacctttg tgcgaagttg ttcctggacc aaatgcgtgc ggtcgccgtg ccccgccaga
     3721 atccattcga accggtagtc gagaagtttc tcgatggaac gcatgagctc gtcatggttc
     3781 cagaccagct ctctgggggc gcccagttgc cggcggtccg attcccacca gagatgatcg
     3841 ccggaaaata agaaccggct ctgatggagt agcacgctgc tgccgggcgt gtggcccggg
     3901 acaggaatca tgtggaactc cggagcgagc agaaccggct ccgtggcgtc cacgatccat
     3961 tccgcatccg gcattgcgtg cgcatccgcc cggtgaatga tccgccgggc cccgaagcgt
     4021 ttcgcataca tcgctgcgtc cgccacgtcg tcttcatggg tcaagaagat gttcctgatg
     4081 ccgccccgtc gctcgaacat ctcgacgaga tgcttgatat agcggggcga atcgatgagc
     4141 cagttgccct ccggatgttg aacgaaataa ctgttggcgc cgaaggattt ctccgagttg
     4201 aacccgcaat agtacacgtt ctcgtcgagg cgcagaggga aactcgccat cgcggcttgc
     4261 actcgtgcgc gatccccctg ttctgtcccg atcgaaccga ccggacaggc cagcaaggcc
     4321 tgatacgcct gctggacctg tctgtcgcct tgcggctgga tggttacggc cgaatactcg
     4381 ccgatctctt caaaactgtt cggcgcaagt tgccggcagg tgtcgcagtt aatacaggtg
     4441 gaatcgacat aaaagttccc cgccacattc gcgtcgagtc gtttctttcg gtcggccatg
     4501 ggaccagcct ttatacggtg acgccgggag ccgacttccc gttcttcgag cgatcgaccg
     4561 cgcgccacag ataccaggag gcggcggttc gatagggctt ccagcgttct ccgtagtgca
     4621 acacttcttt cggcgtcggc atgctgcggc gcccgtaggc aatgcgaaac ccgttgcgga
     4681 caccgaagtc gtggaccggc aggacatccg gccggcccaa ttggaagatc agcagcatct
     4741 cgaccgtcca ccgtccgaca cctcgcacct cgatcagccg ttcgataatg gcctcatcag
     4801 tgagctgcgt gatcacacga gttgtgagga ccgttccctc gagcgctttg gtggccagat
     4861 cacgcagcgc cgccactttc gcccgcgaga atcccgcgcg tctcatggcg cggacgttcg
     4921 ccccgagcac ctcggcggga cggggaaacc gccggccggg aaacaatgcg acgaaccggc
     4981 gcagaatact ttccgcagcg ttgtcgtgga gctgctgata ggcgatcgcg cgcgccagcg
     5041 attcgaacgg cgatcgcccg acccgctgca tcaacgtaaa gcggccgatc tcgtgaatga
     5101 gccggcgcat gaccggatcg acccgagcaa ggtgtctggt tgcggatacc tgaaggacca
     5161 tctgttaccc gatcaacggt gatctacggc cccagtgcgc cgagaacaca aagtcgtcgg
     5221 ccggctgacg gtcacgaggc ttgacttccc gctcgcgcag atagtcagga aggacagcgg
     5281 ggtcgcccgg atatccgacg gcgatcatcg cgaccggatc gaaacccagc gggatggagc
     5341 agtccgcgcg agctttctcc acgtcgaagc cggccatctg gtgtgcaaag agtcccaatg
     5401 cggttgcctg caacacgaga ttctccactg ccattcctgt gtcgtgaaac gcgtgcctgt
     5461 tcggttttcc attctcttca aagttcaggc tcgcaacgga aaggatcagt gccggggcac
     5521 ggtaggccca cttgcggttc ccttccaaca gacaggcaag aagccgggca aaatcttcct
     5581 cctgttcctt gatggcaaca agaaaccgcc aaggttgctc attgttggaa gaaggcgccc
     5641 accgagccgc ttcgaacagg ctgcgcaact tgactgcttc gactggctga tccgaaaacg
     5701 cgcgagggct ccagcgccgg gcgatcagtt catgaattgg agatgctgtc gtcgcgggtt
     5761 tttccatgag cgtggctacc ctttcccgtc gtccttcact tgatccttgt cgggcgacca
     5821 gccgcctcca agcgccttgt acagttggac cactgacacc aaatggagac gatgggtggc
     5881 ggcaagggcc aattccgcat caaaaaggct ccgctgagca atgaggacgt ccaagtagtt
     5941 cgccaggccg ccttgatatc gcagattggc gagccgcaag gccgaccgca gggcattgac
     6001 ctgccgttcc tgggcggcag cctgctcacg agcggtgctg acccctacca acgcgtcatc
     6061 gacttccctg aaggcgacga ggatcgtctg ttcgtattgg gctaaagcct gtctggcttg
     6121 ggcttcggcc gctttctgtt ggaagcctaa aatctgtgca ttgaacaatg gtccggtcaa
     6181 gctggggcct gcaacaccga agaaggtctc gttggccaca agttgggtca attgtggact
     6241 ggctacaccc agaatgccgg tgatgctgag cttgggaaag cggtctgcct tagccatgcc
     6301 gatcctggcc gtcgccgctg ctaggccttg ctccgcctgg accacatcgg gccggcgttg
     6361 gagcagttcg gaagggaggc cagctggcac tactggtggc atcacctgct cggtgagcga
     6421 ccgacccctg gagatttggg cgggattcct ccccagcagc acgctgagct cattttcttt
     6481 ttgaaccatc tgacgcttca gctccgcgat gcgagccgcg gcattttccc gctcggcctc
     6541 gaattggtcg acatccagcc gattggtgag cccctgccgg agccgcgcct ggccgattct
     6601 caccgattct tcccaggcct gcagcgtgcg catggcgatg tccaattgca tgtcgaattg
     6661 ccgaagatcg aaatagccct gggccacccc gcccacgatt tgcacgatga gggctcgtcg
     6721 gttctcttcc ctggccaata aatcgccgag agcagcttcg ttggaacgac ggatccgtcc
     6781 ccagatatca agctcccagg caagattccc ctgcgcatag taattgaacg ggttggggaa
     6841 cccggggaag gcaatattct tcgtgttgcc gaaggtcggc gcattgaccg ttgcattcat
     6901 ctgaggggca aagtcggttt tggcgataag cgctcgcgcg gcgaactcat caactgtcgc
     6961 gacgctccgc ttaagatcct tgttctcctc cagcgcgata cggatcaact tttggagctc
     7021 ttcgtcatga tagagttccc accaaggcgc attggcgagg gagggtaagt cttttccttc
     7081 cccggccatg cgaaaggagt cggaggtggg aatgtcgggg cgagaatagt ccggccccat
     7141 ggcacaggag accaacagca ccgaaagcgc taccaggcat aggccacgca tatcaatgtt
     7201 ctccttccgc tgatgactgg ggcggtatcg cgcgctcctc tccggttccg accactagtg
     7261 cgtggcttgc gctaccccga ctgaacttcc gcaccaggac gaaaaagagc ggcacgaaga
     7321 aaatggccaa gaaggtcgct gcaagcatcc cgccgaacac gccagtcccg atcgattgcc
     7381 ggctggcagc ccccgcaccg gtcgcgacaa ccagcggcaa cacaccaagg atgaatgcca
     7441 tcgatgtcat gatgattggg cggaagcgga gcttggcggc ttcgacggcc gcctccctcg
     7501 gcgacatgcc ggcctcgtgg cgatgcttgg cgaactccac gatcaggatc gcgttcttcg
     7561 ccgcgaggcc gatcagcgtg accagcccaa tctgaaaata gatgtcattg ctcaagccgc
     7621 gaagccacac ggcgagcaag gctccgaaga cgccgaacgg cacggcgagg atcacggcga
     7681 agggaacggt ccagctctcg tactgagcgg ccagcacgag aaacaccatc aggagtccga
     7741 atgcgaacgc ataaatcgat tgtcgtccca ccaaacgttc ttggtatgag atcccgctcc
     7801 agtcggcacc atagccctgg gggatcaaga cttctttcgc cacccgatcc agcgcatcga
     7861 gtacctggcc ggagctgtat cccggggcgg cagtacccag caccaaggcg gtgttaaatc
     7921 cgttaaaatg cgtcaccggg tccggtccgc tcgtgtactc tgtcgtgacc accgtgtcga
     7981 gcggaatcat ggatggtccc tgtccactca gagcgcgcac ataaatcttg gaaatatcct
     8041 cgggtttgga tcgatactgg gcctctgctt ccgtttgtac ccgatagaca cgaccgaatt
     8101 tcaggaagtc attcacgtag aggttgccga aaaaggcctg cagggtatcg aagacctcgg
     8161 caatgggaac ccccaacgcc ttcgcgcgct cccggttcac atgcgcatag agtcggggag
     8221 agctcacgcg aaaactcgtg ccgattgcgc cgatggccgg gtccttcctg gccttggcga
     8281 ggaagtcctg aactacggcg gcgaacttct tgaagtctcc tccgctaggg tcctgcagtt
     8341 gcaaggaaaa gcctcccgtc gcgccgagcc ctctgatgga aggcgcgttg aaggccagga
     8401 tgagcgcctc ggggatcttc gcaaattctc cgtaggctgc accaatcagg gctttcacat
     8461 gttggtgcgg ttccttccgt tgattccaga ggtgcaacgg cacaaacatt gtggctgtat
     8521 tgggcccccg cgtgttgaag atgaagttga ggccggacat cgcatccgtc gaatggatcg
     8581 cggaattcgc gaggaaatag tgctccacct tgctcaggac ggcatcggtc cgctgcttgg
     8641 acgctccgtc cggaagctgg aacatggtga tgaaatagcc ctgatcttcc tccggcaaaa
     8701 agcttgaagg gatcactctg aacagaccga acacgaacac catgagcact ccgaacaccg
     8761 ccaaaacaag ccctgaccgt ttgagggcgc ccgccacgct ggccgtgtag cgattctgcg
     8821 tccatgcgaa gagcctgttg aaaagcgcaa aaaagcctgt gcgttggccc ttgcctcccg
     8881 gcttcaagac gagggcgcag agggccgggc tgagcgtgag ggcgacaaat cctgagatcg
     8941 tcacggacat cgcaatggtg atcgcaaatt gcttgtagag ctggccggtg atgccgccca
     9001 ggaagcccac cggtacgaat acggcgcaga gcacgaggac gatagcgatc acgggcccgg
     9061 tgacttcggt catggccctc ttggcggctt ccttggatga gcaacgttct tcctccatgt
     9121 ggcgctcgac gttctcaacc acaatgatcg cgtcatcgac gaccagtccg atcgccagaa
     9181 tcatgccgaa gagggtcaag gtgttgatgg aaaacccgag ggcctgcatg ccggcaaagg
     9241 tgccgatcag tgagaccggc accgccacgc cgggaatcag ggtggtgcgc cagctttgga
     9301 gaaagagata cacgaccaag acgaccaaga tcatcgcttc acccaaggtc ttgacgacct
     9361 cttcaataga cacctcgatg aatctggtcg tgtcgtatgg aacgtcccac gacactccgg
     9421 gcggaaaact cttggctacc tcccccatct ccttgcgaat ccgttttacg ctatcgaggg
     9481 cgttggctcc cggtgacagg aatgtcagga gaaacacatt gggcttcccg ttccatcgcc
     9541 cttcgagtgt gtacgattgc gctcccagct caaccttcgc gatgtctttc agacggatca
     9601 tcgacccatt gggcagcgcc cggacaatca tctcctcgaa gtccttcacg tccgtgagac
     9661 ggcccagcgt catcactgga atcgtcagtt ctgtgccttt ggtcgctggc tcccgcccaa
     9721 tggtgccggc tggaaagtcc cgattctgct cgcggacgac attggcaata tcggtcgggg
     9781 tgaggctcag ctgcgccatc cgaactggat ccaggatcaa ccgcatcgag tagttctgct
     9841 gcccgaacac gtaggcatct cccacgcctt tgagccgttt gagattgtcg atcactcgga
     9901 gaatggcgta gttggagaga aagaccgtgt cgtgtctggg gtcggttgaa ctcagcgcga
     9961 tgactgccag aagatccggc gagacttttt tcacgctgat gccttggcga atcacctcat
    10021 ccgggatctg cggttccgcc agcttttccc ggttctgggt ctggacttgc gcgatgtcca
    10081 catccgtccc gatctcgaag gtgagccgga tggtcatgtg gccatcattg gtgctggtgg
    10141 agtcgtagta gaggagattg tcaatgcccg ggagctgcac ttcgatagta cgcgccaccg
    10201 agtcagcgac cacctctgcg ctcgcaccgg gatagtcggc ctcgatttgg acaaccggtg
    10261 gcgtaatttc cggaaactgc gcgatcggaa gggcattcaa agacaccaac cctaccacca
    10321 caatgatgat ggagaggacc gaggcgaaga tcggtcggtc gatgaagaaa tgcgagatca
    10381 ctttgtcggc tccgtcttcg tggccgcagg ctggtccacc ggggtggcct cggcgacgct
    10441 agacgaaacc ggtttgaccg gagctcctgg cacaattcgt tggaatccgt ccaccaccac
    10501 caattctcct gcttgcagtc cctcttcgat gagccactgg ttgccctgcc agctggtggc
    10561 ttggacatcg cggacctcca ccttgccgtc agccaagacc acaaacagta cgggtccctt
    10621 cggtccctgc tggaccgctc gttggggaac caagatggcc ccggttttta ccgtgccttt
    10681 gaagcgaacc gtgacgaact gtccgggaac caggatctgg tctggattgg ggaacgtgac
    10741 ccgtgctggc ctggatcctg tctcggtgcg catgcctaca tcgagtagat ccagaactcc
    10801 ttcatgggga tacgtcgtgc tatccaaaaa gatgatcgtg ccccggagct ggtacacacc
    10861 cgggtgttca atcctcttcg ccgcaatgtc ccgtcgccgc ttcaagagaa agctttcggg
    10921 ggcgctgacg gtgacgtaca tggggtcgat ctgatgaacg atcgtgagga gatcggtctg
    10981 ggccgaaacc aagcgtcctt cgtaaactcg agtccgttca atcatcccgt taatcggagc
    11041 gatgataaag gtgttatcaa agtcgaactt tgccttgacc agatcggcct tcgccccgtc
    11101 cagcgccgcc gctgccgcca gctcttcggc gactccatca tcaacgtcct tctggctcac
    11161 cgcctgctct gccaggagag gcttcacgcg ggcaagattt tgcttggcct gcaccagtcg
    11221 cgcctcagcc tgagccacct tcgccttcgc gctgagcaag gccgctttga acggaaccgg
    11281 gtcgatttga tagagacgat c
//
LOCUS       DATIFR010000212         8769 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_719953, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000212 DATIFR010000000
VERSION     DATIFR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719953"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VLD60_12240"
     CDS             <1..756
                     /locus_tag="VLD60_12240"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSB45778.1"
                     /translation="ALKADRCVIFTDVDGVYTTDPNIVPSARRIDKISYEEMLEMASL
                     GAKVLQSRSVEFAAKFNVPLEVNSSFKEGKGTLVTREDEDMEAVAVSGVTGDRNQAKI
                     TVVGVPDKPGIAAALLGPVAKANILVDMIIQNVSHASLTDFSFTIPRVDVKRAMPIIQ
                     DVAKDIGAKSVSVTEAIAKVSLVGVGMRSHSGVAAKMFEVLSREGVNIMMISTSEIKI
                     SCVIDEKYLELAMRALHAAFGLDQAPTGDRSAK"
     gene            813..2465
                     /gene="cimA"
                     /locus_tag="VLD60_12245"
     CDS             813..2465
                     /gene="cimA"
                     /locus_tag="VLD60_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HSB45779.1"
                     /translation="MAKKASRSRTPTGKSSVRSASAAVPSPAKSALLEIYDTTLRDGA
                     QAEDVSFSAEDKVRVAQRLDELGVHFIEGGWPGANPKDIEYFRMIKETPLTHATVVAF
                     GSTRKASNPVQKDPNIQALLDAETKVITLFGKSWSLHVTDALGIALAKNLEIIGDSVA
                     HLLSKHRRVFYDAEHFFDGYKTNPDYALETIRRAVEAGAERVILCDTNGGTMPWEIKE
                     ICKVVQRECPVPLGIHAHNDCEMAVANSLVAIEMGVVQVQGTINGIGERCGNANLCSI
                     IPNLQLKMKRPALGERLTHLKEVSGFVTEIANLMPNKHQPYVGDSAFAHKGGVHIHAV
                     LKNPATYEHIVPALVGNRQRMLVSDYAGRSGLLEKVEAYGIKLDKDHSKVQELMATLK
                     ERENEGYQFEGAEGSFELLMRKAMGTHTPSFQLLGFRVIVEKKQDHGGSTSEATVMVK
                     VGEAVEHAAAVGAGPVNALDHALRKALEKFYPQLREVKLLDYKVRVLSANRGTESKVR
                     VLIESGDHKDKWGTVGVSENIMEASWQALADSIEYKLLASDR"
     gene            2605..2880
                     /locus_tag="VLD60_12250"
     CDS             2605..2880
                     /locus_tag="VLD60_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HSB45780.1"
                     /translation="MRKADIANEIYKQVGISKNEAADIVELVLNMLKAVLQKGDSVKI
                     AGFGNFVVRSKGARKGRNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            2926..3273
                     /locus_tag="VLD60_12255"
     CDS             2926..3273
                     /locus_tag="VLD60_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSB45781.1"
                     /translation="MGNEPRLGSKVFYKIGEVSQMTKLPAYVLRFWESQFPFLKPKKS
                     RGNQRLYVRRDIETVLEVKRMLYEEGHTLEGVKRYWARRGRVSVQRLKPKELAQKLRG
                     NLQAILKILESHS"
     gene            3368..3444
                     /locus_tag="VLD60_12260"
     tRNA            3368..3444
                     /locus_tag="VLD60_12260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3402..3404,aa:Pro,seq:ggg)
     gene            3558..4376
                     /gene="surE"
                     /locus_tag="VLD60_12265"
     CDS             3558..4376
                     /gene="surE"
                     /locus_tag="VLD60_12265"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HSB45782.1"
                     /translation="MSHIRPRILITNDDGIQSPGLTILAKCLAKLGEVWVIAPDRERT
                     AVAHAVTLHKPLRVQQVGKRVYAINGTPVDCVNLALLNIMPRRPHLLVSGINKGVNLG
                     DDVLYSGTVSAAVEGTILGVPSMAVSQEGRDQFQFATGAHYAMRVARLILQRGLPDET
                     LINLNVPHRTIRAITDVRVTCLSRRRFENPIIEKVDPHGRTYYWIAGTRVSWSRSKDA
                     DHEAIEEGAVSLTPIHLDSTHYGVLDQLRTWESAIRGSSKRMASSKAKKRRERH"
     gene            4383..5021
                     /locus_tag="VLD60_12270"
     CDS             4383..5021
                     /locus_tag="VLD60_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSB45783.1"
                     /translation="MGNLIEAIITILSRFVINTISTFGYTGILLTMAIESACIPLPSE
                     IIMPFSGYLVTTGQFTMLGVTLAGAIGNVVGSIVAYYAGVWGGRPFVERYGPYMLISH
                     KDLEMADRWFAQYGEAAVFFSRMLPVVRTFISLPAGVARMNFPRFVIFTFVGALPWCY
                     LLAYIGVKMGEEWEHLRDYFHQFDIVIGLFLALAAGYFLWSHWPKRSLTPGS"
     gene            5028..6506
                     /gene="cysS"
                     /locus_tag="VLD60_12275"
     CDS             5028..6506
                     /gene="cysS"
                     /locus_tag="VLD60_12275"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSB45784.1"
                     /translation="MLTFFNTLTGRQEPFTSIEPKRVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGYQVDFVKNFTDVDDKIIKRANERGVSCDAITSEFIGAYYQDMGK
                     LGIKRATKEPKATEHMAEIVGITETLIKKGLAYQVDGDVYFQVDKYPAYGRLSKRKLE
                     DLQAGARVDVDERKRHPMDFALWKSSKPGEPSWPSPWGAGRPGWHIECSAMSIKHLGE
                     TFDIHGGGMDLIFPHHENEIAQSCGATGKEFARYWIHNGFVQVNQEKMSKSLGNFFTI
                     REIFEKSEWSDEITGEVLRYFLLSTQYRGPLDFSDQSLKEAKNALNGFYDLFNRLAEP
                     GQGAESGEAQLHTRVDETRRAFEGAMSNDLNTPVALAAFQTLRSDVNKLLGVGLSTQG
                     RQAARQAFRSLGAVLGLFQLDKWQFGEQAIGLTGVEAKATSGQLSAQSKLSDEQIERL
                     VAERLDARKERNFARADEIRKSLASQGIIIEDKPDGTSRWKR"
     gene            6481..7269
                     /gene="rlmB"
                     /locus_tag="VLD60_12280"
     CDS             6481..7269
                     /gene="rlmB"
                     /locus_tag="VLD60_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HSB45785.1"
                     /translation="MVPVAGSDSQPELLYGLHAVREALKAGTRPLQRLLLLRADRQFA
                     DLAQLAKTKHIPVHIEPPAAFDRLVPDGRHQGVIAYVAAKAYSSAEEILERARFRGEP
                     PFLVILDGVEDPHNLGAVLRTAEATGVHGVFIPERRAAGLTSVVAKVSAGALDHMLVA
                     RVGNLSRLIEELKETGVWVYGVEPTAPTRYTSIDMTGPIALVLGGEGEGIRPGVLKQC
                     DDRISIPMKGNVESLNVSAAVAGVLLEGVRQRDAKRQAPKPIES"
     gene            complement(7256..7531)
                     /locus_tag="VLD60_12285"
     CDS             complement(7256..7531)
                     /locus_tag="VLD60_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSB45786.1"
                     /translation="MARTVKAKSKTSLADLAPPPRKKRPESLTSREQEILELIWAGFK
                     NKEIGQRLKISVKTVEAHRANMMKKLRVSNTAQLLKTAIQGNMIRIR"
     gene            7743..8525
                     /locus_tag="VLD60_12290"
     CDS             7743..8525
                     /locus_tag="VLD60_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSB45787.1"
                     /translation="MADGFSYLIAGTLGALIGSFLNVCIYRLPRGKSIVWPGSHCPSC
                     GKSIEFYDNIPVLSYLWLIGRCRACRQRISIRYPFVEAINAAGYLAILWTFGPTWTTV
                     LYALLFSALLVVAGTDLTHKIIPNVVTMPGIVLGLLGAATVLPVGLLNSLLGVAIGGG
                     ILWALAWASPYLFGKEGMGGGDIKLLAMIGAFLGWKPALLTIMIGSLTGSIIGISLIA
                     LRLMKRDEYIPFGPFLVLGALLSMFFAQPMLDWYQGLLSPPY"
BASE COUNT         1888 a         2311 c         2630 g         1940 t
ORIGIN      
        1 gccttgaagg cggatcgctg cgtcattttc accgatgtcg acggggtcta tacgacggac
       61 ccgaacatcg tgccgtcggc caggcggatc gacaagattt cctacgagga aatgttggag
      121 atggcgagtc tgggcgccaa ggtgttgcag agccgctccg tcgaattcgc ggccaagttc
      181 aatgtgccgc tggaggtcaa ttccagcttc aaagaaggaa agggaacgct tgtgacacgc
      241 gaagatgaag acatggaagc cgttgcagtg tcgggtgtca cgggcgaccg gaaccaggcg
      301 aagatcacgg tcgtcggcgt gccggacaag ccaggaatcg ctgcagcgct cctggggccg
      361 gtcgcgaagg ccaacatatt agtcgacatg atcattcaaa atgtgagtca cgcgtcgctg
      421 acggactttt cttttaccat tcctcgcgtc gacgtgaaga gggcgatgcc gatcattcag
      481 gatgtcgcga aagacatcgg ggccaagtcg gtttcggtca cggaagccat cgccaaggtt
      541 tcgttagtcg gcgtcgggat gcgttcccac tcgggggtgg ccgcgaagat gttcgaagtg
      601 ttatcgaggg aaggcgtaaa catcatgatg atcagcacct ccgagatcaa gatttcttgt
      661 gtcatcgacg agaagtatct ggaattggct atgcgcgccc tccatgccgc gttcggcctg
      721 gatcaggcgc caaccgggga ccgttcggcc aaataatgac gtcatgattt gagcctcgac
      781 aggctcgccc tgctcctgtt accctctctc ccatggcgaa aaaagcgtct cgttcacgca
      841 ccccaaccgg caagtcttca gtccgctcgg cctccgcggc tgtgccgtcc ccagcgaaat
      901 ccgcgctatt ggagatctac gatacgacgc tgcgcgacgg agctcaggcc gaagacgtca
      961 gtttttccgc cgaagacaaa gtccgcgtgg cgcagcggtt ggatgaactg ggggtccact
     1021 tcatcgaagg cggctggccc ggtgccaatc cgaaagatat cgagtacttc cggatgatca
     1081 aagagactcc cttgacgcat gcgacggtgg tggcgttcgg ctcgacgaga aaagcgagca
     1141 atccagtcca gaaggatccg aacatccagg ccctgctgga tgcagaaacc aaggtcatta
     1201 cgctgttcgg aaagtcctgg tcgcttcacg tcactgatgc gctggggatc gccctggcca
     1261 agaacctcga gatcatcggg gactccgtgg cgcatctcct ctcgaagcat cggcgcgtgt
     1321 tctacgatgc cgaacatttt ttcgacgggt acaagaccaa tccggactat gcgctcgaaa
     1381 ccatcaggcg ggcggtggag gccggggccg aacgcgtgat tctctgcgat accaacggcg
     1441 ggacgatgcc ctgggagatc aaggaaatct gcaaggtcgt ccagcgagag tgcccggtgc
     1501 cgcttggcat ccatgcgcat aacgattgtg aaatggctgt cgcgaactcg ctcgtggcga
     1561 ttgaaatggg cgtggtgcaa gttcagggga cgatcaacgg gatcggtgag cggtgcggca
     1621 acgccaatct ttgctcgatc attcccaatc tccagttgaa gatgaagcgt cctgcattag
     1681 gggaacggct gactcatctc aaggaagtat cgggattcgt cacggagatc gcgaatttga
     1741 tgcccaacaa acaccagccc tatgtcgggg attcggcctt cgcgcacaag ggcggcgtcc
     1801 atatccatgc cgtgctcaag aacccggcca cgtacgaaca catcgtgccg gcgctggtcg
     1861 gcaaccgcca gcgcatgttg gtgtcggatt atgcaggccg aagcgggtta ttggagaaag
     1921 tcgaagccta tggcatcaaa ctggataagg accattcgaa agtccaggaa ctgatggcga
     1981 cgctaaagga gcgggagaac gaggggtacc aattcgaagg ggcagagggc tcgtttgaac
     2041 tgctcatgcg caaggcgatg ggtacccata ctccctcgtt ccagttgctg ggtttccggg
     2101 tcattgttga gaaaaagcaa gaccatggag gctcgacttc ggaagccact gtgatggtaa
     2161 aggtaggcga ggcggtcgaa catgccgcgg cggtgggagc tgggccggtc aatgccctcg
     2221 accatgcgct tcgtaaggcc ttggaaaaat tctatcctca gcttcgcgag gtcaaattgc
     2281 tcgattataa ggtgcgcgtg ctctcggcca atcgtgggac cgagtcaaag gtgagggtgt
     2341 tgattgaatc gggcgaccat aaagacaagt ggggcaccgt cggggtctcc gagaacatca
     2401 tggaggccag ctggcaggcc ctggctgata gcatcgagta caagctcctg gcctccgatc
     2461 gctaggctca aagaacctcc ccctcgaatt aattcttgac agcccctcta acctcacgta
     2521 acttatggta aaagtgtctc tcgtgtcagg cggaatgacg gacatttcag ccgacgctgt
     2581 tcaggttgcg caagggggga gagcatgaga aaggcggata tcgcgaatga aatctataaa
     2641 caggtcggca tttccaagaa tgaagcagcc gatatcgtcg agcttgtgct caacatgctg
     2701 aaagccgtgt tgcaaaaggg agattcggtg aagatcgcag gattcggaaa ttttgtcgtc
     2761 aggagcaagg gcgcacggaa gggccggaat ccgcggaccg gcgaagaaat cggcatcacg
     2821 ccccgtcgtg tcgtcacctt ccgtcccagt caagtgttca agaagtacgt caactcgtaa
     2881 cagcgttcgc tcacctgtat caactcccgc cacgaggacc ggtcaatggg gaatgaaccc
     2941 aggctgggta gcaaagtctt ctacaaaatc ggcgaagtga gtcagatgac gaagcttccc
     3001 gcctacgtgc tgaggttttg ggaatcgcaa tttccctttc tcaagccaaa gaaaagtcga
     3061 gggaaccagc gcctctatgt gcgacgcgat atcgagacag tgctggaagt caaacggatg
     3121 ctgtatgaag aagggcatac gcttgaaggc gtgaaacgat attgggcgcg tcgtggccgc
     3181 gtgtcggtcc agcgtctcaa gccgaaggaa ttggctcaga agctgcgcgg gaatttacag
     3241 gccatcttga aaattcttga gtctcactca tgatccaccg aagccgcgga gtctctttcc
     3301 caaccggttg caattgacca gcgttcgagg cgacaatgca caagattaca gtacaggaag
     3361 cacacgtcgg ggcgtggcgc agcccggtag cgtactcgct tggggtgcga gtggtcggcc
     3421 gttcaaatcg gctcgccccg accaatctca acagtgctga gtcttgagtg ctgagaagca
     3481 gagataattt gggcattcag tactcagcac ttggttgtcg gttctgacac agcgagctgc
     3541 caggggcagc tttttttatg tcccacatac gaccgcgcat tcttatcacc aacgatgacg
     3601 ggattcagtc tccaggcttg accatcctcg ccaagtgctt ggccaaattg ggcgaggtgt
     3661 gggtgatcgc gccggatcgt gaacgcacgg cggtcgccca tgccgtgacg ctgcacaagc
     3721 cgcttcgggt ccagcaagtg gggaaacggg tctatgccat caacggcact cccgtggact
     3781 gcgtgaacct cgcgctgctc aacatcatgc cccggcggcc tcatctgttg gtatcaggga
     3841 tcaataaagg ggtgaacctc ggcgacgacg tgctgtattc ggggacggtt tcggctgccg
     3901 tggagggaac gattctgggc gtgccgtcaa tggcggtttc gcaggagggg cgcgatcagt
     3961 ttcaatttgc gaccggtgcg cactatgcca tgcgggttgc gaggctgatc ctccagcgcg
     4021 gcttaccgga tgaaaccctc atcaacttga acgttccgca tcgcacgata cgggccatta
     4081 cggatgtccg cgtaacctgt ttaagcagga ggcgattcga aaacccgatt atcgaaaaag
     4141 ttgatccgca cggccggacc tattattgga ttgccggtac tcgcgtgtcc tggagtcgaa
     4201 gcaaagatgc tgatcatgaa gccatcgagg aaggcgcggt atcgctcacg cccatccacc
     4261 tggacagcac gcactacggc gtcttggatc agctgcggac ttgggaatct gccattcgag
     4321 gaagctcgaa acggatggct tcctcgaagg ccaaaaaacg aagagagagg cactgaggcg
     4381 gcatgggaaa tttgatcgag gccatcatta caatcctcag tcggttcgtg atcaatacga
     4441 tctcgacatt tggctacacc ggcattctct tgacgatggc gatcgagagc gcctgcattc
     4501 cattgcccag cgaaatcatc atgccgtttt ccggctatct ggtcacgacg gggcaattca
     4561 cgatgctggg cgtgacgttg gccggcgcga tcggtaacgt agtcggatca attgtcgcgt
     4621 actatgccgg agtgtggggt ggccggccgt tcgtcgagcg ctacgggccc tatatgttga
     4681 tctcgcacaa ggatctcgaa atggctgatc ggtggttcgc gcagtacggc gaggctgccg
     4741 tatttttcag ccgcatgctg ccggttgtgc gcacctttat ttctctgccg gccggcgtcg
     4801 ctcgcatgaa tttcccgcgc tttgtgatct tcacctttgt cggcgcgctc ccctggtgtt
     4861 atctcctggc ctatatcgga gtgaagatgg gagaagagtg ggagcatctg cgggattatt
     4921 ttcaccagtt cgatatcgtc atcggcctgt tcctcgccct cgccgccggg tatttcctct
     4981 ggtcgcattg gccaaaacgc agtctcacgc cggggtccta ggtcgccatg ctgacatttt
     5041 tcaacacgct gacgggcagg caggaaccgt ttacgtcgat cgagccgaag cgggtccgga
     5101 tgtacgtttg cggcgtgacc gtctatgact actgccacat cggccatgcg cggagcgcac
     5161 tcgtgttcga tgtgctccgg cgttatttgg aatacagcgg ctaccaggtc gacttcgtca
     5221 agaacttcac cgacgtcgac gacaagatta tcaagcgggc aaatgagcgg ggcgtgtcct
     5281 gcgacgccat cacatcggaa ttcatcggcg cctactacca ggacatgggc aagctcggga
     5341 tcaagcgggc gacgaaggag cccaaggcca cggagcacat ggccgagatc gtcggcatca
     5401 ccgagacatt aatcaagaag ggattggcct atcaggtcga cggggacgtg tatttccagg
     5461 tcgataagta tccggcctac ggacgactat ccaaacgcaa gctcgaagat ctgcaggccg
     5521 gcgctcgtgt ggacgtcgat gagcgcaagc gacacccgat ggattttgcg ttgtggaaaa
     5581 gcagcaagcc cggtgagcct tcatggccca gtccctgggg agctgggcgc cccggctggc
     5641 acattgaatg ctcggcaatg tctatcaaac acctgggcga gacgttcgat atccacggcg
     5701 gagggatgga tctgattttc ccgcaccatg agaatgagat cgcacagtca tgcggggcga
     5761 ccgggaaaga gtttgcccgc tattggatcc acaacggttt tgtgcaggtc aatcaggaaa
     5821 agatgtcgaa gtcgctggga aacttcttca ccattcgaga gattttcgag aagtcggagt
     5881 ggtcagacga gattacggga gaagtgttgc gctatttcct cctctcaacc cagtatcgag
     5941 gtccgcttga cttttccgat cagagcttga aagaggcgaa gaatgcgttg aacggattct
     6001 acgatctctt caatcgatta gccgaaccgg ggcagggagc ggaatcgggt gaggcgcaac
     6061 tgcacactcg cgtggatgag acgaggcgtg catttgaggg agccatgtcg aatgatctga
     6121 acacacccgt tgcacttgca gccttccaaa cgcttcgtag tgacgtgaat aaattgctgg
     6181 gggttgggct ttcgacgcaa ggcaggcaag ccgcgcgcca agcatttcga tcactcggtg
     6241 cagtgttggg tttgttccag ttagacaagt ggcagtttgg tgaacaagcc attggcctta
     6301 ccggtgttga ggccaaagct acgtccggtc agctatcggc tcagagtaaa ctctcggatg
     6361 agcagatcga acgattagtt gcggagcgtc ttgatgcaag aaaggagagg aatttcgcaa
     6421 gggctgatga gatccgtaag tcactcgcgt cccaaggcat catcatcgag gacaagcccg
     6481 atggtaccag tcgctggaag cgatagtcag cctgagttgc tctacgggct gcatgcagtc
     6541 cgggaggcat tgaaagcagg gacacgtccc cttcaacgtc tgcttcttct ccgagccgac
     6601 cgacagtttg ccgatctcgc ccaactcgcg aagaccaagc atattcctgt tcatatcgaa
     6661 cctccggcgg cgtttgaccg gttggtgcct gatggaaggc atcagggcgt cattgcctat
     6721 gttgctgcca aggcttacag cagcgcggag gagattcttg agcgggcccg tttccgcggc
     6781 gagccgcctt ttcttgtcat tctggacggt gtcgaagatc ctcacaacct tggcgcggtc
     6841 ttgcggaccg ccgaggcgac cggtgtacac ggtgtcttta tccctgaacg ccgcgcggcg
     6901 gggctgacct ccgttgtcgc gaaggtctcg gccggcgcgc tcgatcatat gctggtagcg
     6961 agggtcggta atctctcccg cctgattgaa gaattgaaag agaccggagt ctgggtttac
     7021 ggcgttgagc cgacggcgcc gacgcgatac acgtctattg atatgacggg gccgatcgcg
     7081 ttggtgttag gaggagaagg agagggaatc cgtcccggcg tgctgaagca atgtgatgat
     7141 cgtatcagca tcccgatgaa ggggaacgtc gaatcgttga acgtctcagc agctgtggcc
     7201 ggggtgctct tggaaggagt gcggcagcgc gacgcgaagc ggcaagcccc gaagcctatc
     7261 gaatcctgat catgttgcct tgaatggctg tcttcagcag ctgcgccgtg ttcgacacgc
     7321 gcagcttctt catcatattg gcccgatgag cctcgacagt cttcacgctg atcttcagtc
     7381 gttgtccgat ttctttgttc ttaaacccgg cccaaatcaa ttcgagaatt tcctgttctc
     7441 ggctggtcaa cgattcgggc cgtttctttc gtggtggagg agcgaggtca gcaagagagg
     7501 tcttggactt tgccttgacg gttcgtgcca tctttcctgt tctcctttga tcgtgagagt
     7561 attacgttat aattcaaccg aaggcggagt tgctggagaa ccttccgatc gtctacgaga
     7621 cggaattata ggaactcctc aaggccatgt aaatcaatgc ataatggccc tagtgtaaat
     7681 acgtattcag ttatcgaccg taccgatcaa tccgtacgga gcattcgaca ggatcttgta
     7741 ctatggctga tggattttcc tacctgatcg caggcactct tggggccttg atcgggagct
     7801 ttctcaacgt ctgcatctac cgacttccgc gcggaaaatc gatcgtctgg cccggctccc
     7861 actgtccatc ttgcgggaag tcaatcgagt tctacgacaa tattccggtg ctgagctatc
     7921 tctggctgat tggtcgctgt cgggcatgcc ggcagcggat ctcgattcgt tatccgttcg
     7981 tcgaggcgat caacgccgcc ggctatcttg ccattctctg gaccttcggc ccaacgtgga
     8041 cgaccgtgct ttacgccttg ctcttttcgg cgttgctggt tgtggcggga acggatttga
     8101 cgcacaagat catacccaat gtggtgacta tgccagggat tgtgctgggt ctgctcggtg
     8161 cagcgacggt cttgcccgtt gggttgctca attccctctt gggtgtggcc atagggggag
     8221 gcatcctctg ggcgcttgcc tgggcgagtc cctacctgtt cggcaaggag gggatgggcg
     8281 gtggcgatat aaaattgctg gcaatgatcg gtgcattctt gggatggaaa ccagcgttat
     8341 tgacgatcat gatcggatcg ctcaccggtt cgattatcgg aatcagcttg atcgcattga
     8401 gactcatgaa acgtgacgag tacattccgt tcggaccgtt cctcgttctc ggggcgctcc
     8461 tgtcgatgtt ctttgcccaa ccgatgttgg attggtacca aggtttgctc agccccccgt
     8521 attagtctcc tacttcagcg acgatcctgt atctcttcct tcgttctcct gtatccgaat
     8581 ctctttggtg cggaagccta gtcttcagtt gtcgcgcaga aggcacggcc cagcttggcc
     8641 gcgtcagaga tatcgaagtc tcgccagttg cagagatctc cctgtggaca agaaagatgg
     8701 attgccgcgc cgggcagggc ggcacgggtt ttggtattcc gaaagttcgt tatggcgaat
     8761 gagcctatc
//
LOCUS       DATIFR010000213         7494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2250209, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000213 DATIFR010000000
VERSION     DATIFR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250209"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..864)
                     /locus_tag="VLD60_12295"
     CDS             complement(208..864)
                     /locus_tag="VLD60_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSB45788.1"
                     /translation="MSSVWYGFLWVLARFVAWLCFRYRVVGTVPRTGGLLIAANHASY
                     FDIPLLGCGMTRRAWYLGRSDLFPIPGSKAILQWLGWIPLRLGRLDRKAFDKAIALIK
                     QGKVVVIFPEGSRSLDGQLQEAKPGLGMIVAQTGCPVVPAYLKGTFEVLPSGAKWPRF
                     RRVTVRFGEPVTFPKVEQNGENKKFYQDVSQTVMERIAALGEVEPPHHRASAGARNAE
                     "
     gene            complement(861..1520)
                     /gene="cmk"
                     /locus_tag="VLD60_12300"
     CDS             complement(861..1520)
                     /gene="cmk"
                     /locus_tag="VLD60_12300"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSB45789.1"
                     /translation="MIVAIDGPAGVGKSTVAKQLAFRLGYLYLDTGALYRAVAWATLR
                     NGYDPADADAVAGLLPNLSIQMQFKNETVTVSVNGKDVSGELRTPAVSAAASVVSAIP
                     AVRAWLLPVQQQISLQGSVVAEGRDIGTKVFPAAEAKFFLEADPTVRAERRHRELVAA
                     GHGAAIEQTSAELAGRDNRDRSRTVAPLVPAEDAHHIDTSTLSAEEVVNQMLAVVAAK
                     L"
     gene            complement(1578..2900)
                     /gene="aroA"
                     /locus_tag="VLD60_12305"
     CDS             complement(1578..2900)
                     /gene="aroA"
                     /locus_tag="VLD60_12305"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSB45790.1"
                     /translation="MTSLTITPGRPLRGTITVPGDKSITHRAIILTALAEGTGTIKGY
                     CRGEDCLNTMRAMQSLGVKIDESVEELRVQGKGLWGLTEPAGIVDCGNSGTGIRLLTG
                     LLAGQDFFTVLTGDASIRRRPMGRVVKPLREMGATIAGRKGGELAPLAVSGTTLRAIR
                     YASPVASAQIKSSLLLAALFAKGTTHLSEPKPSRDHTERLFQFYGLPLKREGTTLTME
                     GRPSIGWTAVPALTIPGDLSAAAFFIVGATIVPGSDVTVTGVGINPTRTGLLEVMNRM
                     GADIQLLNQREEAGEPVADIRAKAAPLRGVTIGPDLIPQTIDEFPILCVAAAVAEGQT
                     VITGAEELRVKESDRIATMATELRAMGAQITERSDGLAVQGIGQPKANGRLTATSGQS
                     HGDHRVAMSIAIAGLTASTETVIQDTDCIETSFPGFDRKLLELLTDSR"
     gene            complement(2908..3798)
                     /locus_tag="VLD60_12310"
     CDS             complement(2908..3798)
                     /locus_tag="VLD60_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HSB45791.1"
                     /translation="MAVHFTQVAIIGVGLIGGSLGMILRRKGMATRVIGVGRRVENLK
                     TAVELGAIDRYVSDPKEGVTGSDLVVLATPVDTYDRHLKDWASCLKRGAIVTDVGSVK
                     GQLVEQSERSMPAGVHFVGGHPIAGKEKTGAAAGSEQLFAGARCILTPTKATNQQALE
                     QVRTMWQETGAAVLTMDPYLHDQILGAVSHLPHAAAFALMNALIEIRTKIPGLDLAGH
                     SGGGLRDTTRIAASSPEMWRDIFLWNRDNLVAFIEAYERSLGQLKQLIRAGDAAGIEK
                     ELERAKQERDKFPARPPVTA"
     gene            complement(3873..4886)
                     /gene="aroF"
                     /locus_tag="VLD60_12315"
     CDS             complement(3873..4886)
                     /gene="aroF"
                     /locus_tag="VLD60_12315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSB45792.1"
                     /translation="MIIVLRPDASEREVDHIVDRLRELGLKSQLSTGQERTIIGVIGD
                     DRILQNQPLTALPGVESVLPILAPWKLVSREFKKEGTIIDVGGVKIGGNKLAVMAGPC
                     AVERLELTVGIAHEVKAAGASILRGGAYKPRTSPYSFQGLGREGLDYLVEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNRNVMLCERGIRTFETQYRNTLDLAAIPTLKELSHLPVIVDPSHATGK
                     WNLVAPMSKAAVAAGADGILIEVHSNPECALCDGEESIKPSKFKELMQDMRKIAIAVG
                     REV"
     gene            complement(4905..5987)
                     /gene="hisC"
                     /locus_tag="VLD60_12320"
     CDS             complement(4905..5987)
                     /gene="hisC"
                     /locus_tag="VLD60_12320"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSB45793.1"
                     /translation="MPLHVHPDIAALSPYVPGKPIEELQRELGLERVIKLASNENPLG
                     PSPKALAVLNEGAATLHRYPDGGAYRLREALADRWKVSSDHIILGNGSDEILGLLART
                     FLAPGDEAVMADHTFVIYKMEVTAAHGRAVTVPLKQWRHDLSAMADAVTPRTRLLFVC
                     NPNNPTGTMVSAGEVDRLLTRLPEHVIVVFDEAYFEYVRSSEFPDSMAYVKQGRNAIV
                     LRTFSKIYGLAGLRIGYGVTTPEITNFLNRVRPPFNANSLAQRAALAAMSDDEHVAKS
                     RAINEAGMEQVVNGLRSLGFSPIPSEANFVYVDVGQDGRNVFEALLRRGVIIRHIEGR
                     MVRVTIGQAEENQVFLTALARVVQAG"
     gene            complement(5992..7068)
                     /gene="pheA"
                     /locus_tag="VLD60_12325"
     CDS             complement(5992..7068)
                     /gene="pheA"
                     /locus_tag="VLD60_12325"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSB45794.1"
                     /translation="MPGDLSAYRKEIDRLDDEILRLLNERSKSVIEIGKLKKQQDADA
                     NLHTPAREAEIIERLVKQNTGPFPSEAIRPVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACTQKFGSSAQYVPVNSIKEVFSEVERGRANFGVVPIENTTEGVVNHTLDMFIDSN
                     LLIYGEVLQEVSHHLMSKSGAAEEIKKICSHPHAIAQCRSWLETNMPHVPVSEVASTA
                     RAAEMCLDDPSVAAIASELAAQLYGLKVIKSRIEDNLNNFTRFLVLSQKPPQRTGKDK
                     TSLMLSIKDKVGALYDLLRPFASHGLSMTKIESRPSRRKAWEYIFFVDIEGHIEEERV
                     KKAVEEITGRCLFMKVLGSYPAHS"
     gene            complement(7378..>7494)
                     /locus_tag="VLD60_12330"
     CDS             complement(7378..>7494)
                     /locus_tag="VLD60_12330"
                     /inference="COORDINATES: protein motif:HMM:NF017317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HSB45795.1"
                     /translation="DVHEPYLIQAGFLDRTGRGRQATKLAFDHFSKPAAQLF"
BASE COUNT         1553 a         2351 c         1992 g         1598 t
ORIGIN      
        1 ccgatgtcga cgaccacttt gtccttggtc aaggccacga cgcggccctc ggtgatggtc
       61 ccctcctcga ggttcttgaa ggtttcctcg tacaaggcgg ccaaggcatc gcggtccaat
      121 tgctgctcac tggatttcga aaccgtactc atgggctatc ctaatcttcc gactgctgtc
      181 gggcgctgat atggatgaca ccggaactca ctcagcattg cgagcaccgg cacttgcgcg
      241 atggtgggga ggctcaacct cccccagtgc ggcaatccgc tccatcactg tttgactgac
      301 gtcctgataa aactttttgt tctctccgtt ttgctctacc ttgggaaagg tcactggttc
      361 cccaaatcgc acggttaccc gacgaaacct cggccacttg gctcccgaag gcaatacctc
      421 aaacgtcccc ttcaaatacg ccggaacaac ggggcaaccg gtctgtgcga caatcatccc
      481 caatcccggt tttgcctcct gaagttgacc gtccagactc ctgcttccct cggggaaaat
      541 gacgaccacc ttcccctgct tgatcagggc aatggccttg tcgaaagcct tgcgatccaa
      601 gcgccctaac cgcagtggaa tccaacccaa ccattgcaaa atcgccttcg atcctgggat
      661 ggggaacaaa tcactgcggc ccaaatacca ggcgcgtctc gtcattccgc aaccaagcaa
      721 cggaatgtcg aaataactcg catggttggc tgcgatcaac agaccgccgg ttcgaggaac
      781 cgttccgacg acccgatagc gaaagcacaa ccaggcaaca aaccgtgcca acacccagag
      841 gaaaccgtac cagaccgaac tcacaacttg gccgcgacaa cagccagcat ttgattgacg
      901 acttcctccg cagacaatgt cgaggtgtcg atatggtgcg cgtcttcggc cggcacgagc
      961 ggcgccacag tgcgggaccg atcccggttg tcgcgtccgg ctaactcagc gctcgtttgt
     1021 tcaatcgctg cgccatgacc cgccgcaacc agttctcgat gccgacgttc tgcacggacc
     1081 gtcggatcgg cttcgagaaa aaacttcgcc tccgcggccg ggaagacctt cgtgccgata
     1141 tcacggccct ccgccaccac tgagccctgc agactgatct gctgctgcac cggaagaagc
     1201 catgctcgca cggctggaat tgccgacacc actgaggcag ccgcggacac ggccggcgtt
     1261 ctcaattctc cagatacgtc cttcccgttg actgaaacgg tgacggtttc attcttgaac
     1321 tgcatctgaa tcgataagtt cggcaggagt cccgccacag catcggcatc ggctggatcg
     1381 tatccgtttc gcaatgtcgc ccaggcgacc gcccgataaa gcgcaccagt atccaaatac
     1441 aaatagccca accgaaaggc cagttgcttg gccacggtac tcttgcccac ccccgctggt
     1501 ccgtcaatcg ctacaatcat ccgatcgaat ccctctctct atgcagagct cattactaat
     1561 gagccgtatt aaagtggtca acgactgtcc gtcaacaatt ccaagagctt gcgatcgaaa
     1621 ccaggaaacg atgtctcgat acaatccgtg tcctggatca cggtctccgt cgacgcggtc
     1681 aagcctgcga tcgcgatcga catggcgacg cgatggtctc catgactctg tccgcttgtc
     1741 gccgtcaacc gcccatttgc ctttggctga ccaattccct gcacagccag cccatcggac
     1801 cgctcggtga tttgtgcccc catggcccga agctctgtcg ccatagtcgc gatgcgatca
     1861 ctctctttga ctcgcaactc ctccgctccg gtaatgacgg tctgaccttc cgcgactgcc
     1921 gccgctacgc atagaatagg aaactcgtcg atggtttgcg gaatcagatc cggtccgatc
     1981 gtcacgccgc gaagcggagc cgccttcgcc cggatatctg ctaccggttc tcctgcttcc
     2041 tcccgctggt tgagcaactg aatgtcggca cccattcggt tcatcacttc gagcagaccg
     2101 gttctcgtgg gattgatgcc aacgcccgtc accgtgacat ccgaaccggg aacaatcgtc
     2161 gccccaacaa tgaaaaaagc tgcggcggag agatcccctg gaatcgtcaa tgccgggaca
     2221 gccgtccatc cgatggaagg cctcccttcc atggtcaagg ttgtcccttc ccgcttgagc
     2281 ggaagcccat aaaactgaaa aagccgctcc gtatggtcgc gcgagggttt gggctcggac
     2341 agatgcgtgg tacccttggc aaagagcgct gccagcaaga gtgaggactt gatctgtgcg
     2401 ctggccacag gagatgcgta gcggatcgcg cggagcgtcg tgcctgacac agccagcggg
     2461 gcaagctctc cgccctttcg cccggctatc gttgcgccca tctcgcgtaa cggcttcaca
     2521 acccgtccca tgggccgacg acgaatggac gcatcgcccg ttaacaccgt gaagaagtct
     2581 tggccggcca gaagaccggt gagaagacga atgcccgttc ctgaatttcc gcaatcgacg
     2641 atgccggccg gctccgtcag accccagagc cccttccctt gaactcgcaa ctcctcgacc
     2701 gattcgtcga tcttcacgcc aagcgactgc atcgcccgca tcgtgttcag gcaatcctcg
     2761 ccgcgacagt accccttgat cgtccctgtc ccttccgcca acgcggtcag gataatggcg
     2821 cgatgcgtaa tcgatttatc gccgggaacg gtaatcgttc ctcgtaacgg ccgacccggt
     2881 gtgatcgtca acgacgtcat caatcctcta cgcggtcacg ggaggtcgag cagggaattt
     2941 gtcccgctcc tgcttcgccc gttccaattc tttttcgatc ccggccgcat cgccggcccg
     3001 aatcaattgc ttcaactgtc ccaatgatcg ctcatacgct tcgataaacg cgacgaggtt
     3061 atcccggttc cagagaaaaa tatcccgcca catttccggg gaactggcgg cgattctggt
     3121 cgtatcacgt aatcccccgc ctgaatgacc cgccaaatcg agtccgggga tcttcgtgcg
     3181 gatctcgatc aacgcgttca tcaacgcaaa cgccgctgca tgcggaagat ggctgacggc
     3241 tccgaggatc tgatcgtgca ggtacgggtc catcgtcagc acagcggccc ccgtctcctg
     3301 ccacatagtt cgcacctgtt cgagtgcctg ctgattcgtg gccttcgtcg gcgtcagaat
     3361 gcagcgcgca ccggcgaaga gttgttccga gccggcagcc gcaccggttt tttccttgcc
     3421 cgcgatagga tgccccccta caaaatggac gccggccggc atcgagcgct cagactgctc
     3481 caccaactgt cccttcacgc tccccacatc ggtcacgatg gcgccgcgct tcaaacagga
     3541 agcccagtcc ttcaaatgcc ggtcgtatgt atcgacaggc gtcgcgagaa ccaccaggtc
     3601 ggatcctgtc accccttctt tcggatccga cacgtaacga tcgatcgcgc caagttccac
     3661 cgccgtcttc agattctcaa cgcgccgccc caccccgatg accctggtgg ccattccctt
     3721 ccggcggagg atcattccca gcgagccacc gatgagcccg actccgataa tcgcgacttg
     3781 tgtgaaatgc acggccatgt atcacatcag ccccgggcgg acgctgaggt tcgaacctca
     3841 gggtttcgcc tttcgggacc ccatcctacc gactacactt cccgcccgac cgcgatggcg
     3901 atcttccgca tgtcttgcat cagttccttg aacttgctcg gtttaataga ttcttcgccg
     3961 tcacacagcg cacattctgg gttcgaatgg acctcgatca aaatcccgtc tgcgcccgcc
     4021 gcgaccgcag ctttcgacat cggcgccacg agattccatt ttccggtcgc atggctcgga
     4081 tcgacgatga ccggcagatg ggagagttct ttcaacgtcg ggattgccgc caggtcaagc
     4141 gtgttgcgat actgcgtctc aaacgtccgg atgcctcgtt cgcagagcat gacgttgcga
     4201 ttgccgcggg acatgatgta ctccgccgac aacaagaatt ccttaatcgt ggccgacaac
     4261 ccccgcttca gtagtacggg tttgtcatag gcgcccactt ccttgagcaa ttcgaagttc
     4321 tgcatgttgc gcgccccgat ctgaatgatg tcggccttct ccagaaacag ttcgatgtcg
     4381 cgcgtatcga gaatctcgct caccaccggc aatccggttt gcttcttcgc ctcaaccaag
     4441 taatccaacc cttcacgacc cagtccttgg aaggagtagg gcgatgtccg aggtttatag
     4501 gcgccgccgc gaaggatgct ggctcccgcc gccttcacct catgcgcgat accgaccgtc
     4561 aattccaaac gctccactgc gcacggcccg gccataacgg cgagcttatt tccaccgatc
     4621 ttgactccgc cgacgtcgat aatcgtgcct tccttcttga actcacggct gacgagtttc
     4681 cacggggcca gaatgggcaa cacactttcc acccccggca gggccgtgag cggctggttt
     4741 tgaagaatgc ggtcgtcacc gatcaccccg ataatggttc gttcctgccc cgtggacaat
     4801 tgcgatttca gtccgagttc acggagccga tcgacgatgt gatcgacttc gcgttccgat
     4861 gcatccggtc tcaacacgat aatcatgctt ccctcacccg ttgttcaacc agcctgtaca
     4921 acccgcgcca gcgcggtcag gaatacctga ttttcctctg cttgcccaat cgtcacccgc
     4981 accatgcgcc cttcgatatg gcggatgatg acgcctcgtc gcagcaaggc ctcgaagaca
     5041 tttcgaccat cttgcccgac atcgacgtac acaaaattcg cctcgctcgg gatcggcgag
     5101 aatcccagcg aacgcagacc attgaccact tgctccatcc ctgcttcgtt gatcgcgcgg
     5161 ctcttggcca catgctcgtc gtcgctcatc gcagcgagcg ccgcacgctg cgcgaggctg
     5221 ttcgcattga acggaggacg cactcgattc agaaagttcg tgatctcagg cgtcgtcacg
     5281 ccatatccga tcctcaagcc ggccaagccg tagatcttgg aaaaggtccg cagcacgatg
     5341 gcgttgcgcc cttgcttcac gtaggccatc gaatccggga actcggaact ccggacatac
     5401 tcaaagtagg cttcgtcgaa cacgacgatc acgtgctccg gcagacgggt aaggagtcga
     5461 tcgacttcgc ccgccgacac catcgtccct gtcgggttat tcgggttgca gacaaacagc
     5521 aatcgagtcc gcggcgtcac ggcatcggcc atcgcagaaa ggtcatgccg ccattgcttc
     5581 aacggaacag tgaccgccct gccgtgagcc gcagtcactt ccatcttata gatgacgaag
     5641 gtgtgatcgg ccatcaccgc ttcatcgccg ggcgccagga atgtccgcgc cagcagaccc
     5701 agaatttcat cagacccgtt tcccagaata atgtggtccg acgagacctt ccatcgatcg
     5761 gcaagcgctt cgcgtaagcg gtacgcgccg ccgtcgggat agcggtgcaa tgtggccgct
     5821 ccctcgttca agacggccag cgccttggga gaagggccca acggattctc attcgaggcc
     5881 agtttgatga cccgttcgag tcccagttcg cgttgaagct cctcgatagg cttgccaggc
     5941 acgtaggggc ttaacgcggc gatatcggga tggacgtgaa gtggcatggt cttaactgtg
     6001 tgccgggtac gaaccgagga ccttcatgaa gagacaacgg ccggtgatct cttcgacggc
     6061 tttcttcacg cgctcttctt caatgtggcc ctcgatgtcg acgaagaaaa tgtactccca
     6121 ggctttcctc ctggacggac gggattcgat tttcgtcatg ctcaagccgt gcgaggcaaa
     6181 tggacggaga aggtcgtaga gggccccgac tttgtccttg atcgagagca tcagggacgt
     6241 tttgtctttc ccggtccgct gtggaggctt ctgagacaac acaaggaatc gggtgaagtt
     6301 gttgagattg tcttcgattc gcgacttgat gactttcaac ccataaagct gcgcggccaa
     6361 ttcagacgcg atggctgcaa ctgagggatc atcgagacac atctccgccg cgcgcgccgt
     6421 gcttgcgact tccgagaccg gcacatgcgg catattggtt tccagccagc tacggcactg
     6481 ggcaatggca tggggatgcg agcagatctt cttgatctct tctgccgcgc cggacttcga
     6541 cattaaatga tgtgagactt cttgaagtac ctcgccataa atcaacaggt tcgaatcgat
     6601 gaacatatcg agggtatgat tcacgacacc ctcggtggtg ttttcgattg gtacgacacc
     6661 gaaattggct cgaccacgtt ccacctcgct aaaaacttct ttaatgctgt tgactgggac
     6721 gtactgcgcc gacgaaccga atttctgtgt gcaggccata tgtgtaaagg tcgctctcgg
     6781 accgagatag gccacctttt gagggccttc caaggaaagg gatgcggaca tgatttctcg
     6841 atagacaggt ctgatggctt cggacggaaa agggccggta ttctgcttca caagccgttc
     6901 aatgatttcc gcttcacggg ctggagtgtg caaattggca tcggcatcct gctgtttctt
     6961 cagcttgccg atttcaataa cgcttttcga ccgttcgttg aggagacgca agatctcgtc
     7021 atcgagtcga tcgatctctt tccggtaggc ggaaaggtct ccgggcatgc acggaccccc
     7081 tccacccgtg ccccatgagt tggacgagcg tccagaggca ggacgggtgt gagcaagaag
     7141 gaaatgatac aggaaccggt ctcggaaatg caaggagacc cctggacttt caggaacttg
     7201 tgcgatcgag gtgcacgatg gattgcatgg ggtggcaagc gggagcgact ggcggttttg
     7261 gcaccgagtg tttcagtcca tggaatctgt cgggctagct gggggagagg ggctgcctct
     7321 gcaatagtgc tctcccgacg ccgacccaac gcagggcctc gaataccgtc ttcgctgtta
     7381 gaacagctgc gcggctggct tgctgaaatg atcgaacgcc aacttcgtgg cctgtcggcc
     7441 gcgcccggtg cgatcaagga atccggcttg aatgagatag ggctcgtgga cgtc
//
LOCUS       DATIFR010000214         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2827804, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000214 DATIFR010000000
VERSION     DATIFR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827804"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1003
                     /locus_tag="VLD60_12335"
     CDS             86..1003
                     /locus_tag="VLD60_12335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSB45796.1"
                     /translation="MSLKRKDLLSLAPLSADEIKLILETADSFKEVTGRDIKKVPALR
                     GRTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLARGVKSSVINAGDGWHEHPTQALLDLYTIRERGMSFEGLRVA
                     IVGDVAHSRVARSNIHALSKLGAEVRVVGPPTMIPMGIDKLGAKVFYNLDDALRGVQV
                     IMMLRLQLERQGRALFPTIREYARLYGLTAERVKLANAGAIVMHPGPINRGVEIAPEV
                     ADSLSSVILDQVANGVAVRMGILYLMSGA"
     gene            1006..>2208
                     /locus_tag="VLD60_12340"
     CDS             1006..>2208
                     /locus_tag="VLD60_12340"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSB45797.1"
                     /translation="MTILIQGGHIIDPGRFNAVGDLLIDQGKVVAIGPNIKAPAGATM
                     IVAKGRLVVPGFVDLHVHFREPGFEYKETIQSGAASAVAGGFTTVCCMPNTNPVNDNQ
                     AITEFILERARAAGLANVLPIGAITKGSEGKELAEIGDLRRAGCVAISDDGKPVMNSL
                     VMRRAMEYALAFDVPVVDHCEDLHLAEGGCMNEGLISTELGLPGIPAAAEDVMVARNV
                     SLAELTGARLHLAHISTVGSVRMVREAKSRGIKVTAEACPHHFTLTEEVVRGFNTLAK
                     MNPPLRTWNDVQAIKEGLRDGTIDVIATDHAPHAAQEKQQGFTEAPFGIVGLETALPL
                     TLALVEEGILTLEAAIEKLSSAPAKTFSLSKGSLAVGADADVAVIDPHVQWEVDPAKF
                     RSKSRNTPF"
BASE COUNT          477 a          567 c          708 g          456 t
ORIGIN      
        1 cgggaagaga agatccaggt tttgctggaa gaggcaggcg aagaagaccg ggtcgtcata
       61 ctcaaggcgt gaaggagacg aagcgatgag cctcaaacgc aaagatcttc tgagcttagc
      121 gcccttgtcg gcggatgaga ttaagctgat tcttgaaacg gccgactcat ttaaggaagt
      181 gaccggtcgc gacatcaaga aagtgccggc acttcgtggc agaaccgtcg tgaatttgtt
      241 tttcgagccg agtacgagaa ccagaacctc gtttgagctg gctgcgaaac gcctgagcgc
      301 ggacgtcatc aacttctccc cttcgtcaag tagcgtggtc aaaggggaaa cgctcctcga
      361 taccgcgcgg aatatcgagg cgatgcaggc ggacattatc gtgctgcgac attcgtcggc
      421 cggggcagca gagacgctcg cgcgcggggt caagtcctcc gtcattaatg ccggcgatgg
      481 atggcacgag catccgactc aagccttact cgatctgtac acgattcgcg agcggggcat
      541 gtcgttcgaa ggacttcgcg tcgccattgt cggtgatgtg gcgcatagcc gcgttgctcg
      601 ctccaacatc catgcattgt cgaagctggg agcggaggtt cgtgtcgtcg gccctcccac
      661 gatgattcct atgggaattg ataagctggg cgccaaggtc ttctacaatc tggacgacgc
      721 gttgcgaggg gttcaagtca tcatgatgct gcgattacag ctcgagcggc aggggcgggc
      781 gttgtttcca accatccgcg agtatgcccg cctatacgga ttgacggcag aacgggtcaa
      841 attggccaat gcgggggcga ttgtcatgca tcctggcccg atcaaccggg gcgtcgagat
      901 cgctcctgaa gtggctgaca gtctgtcatc ggtcattctc gatcaagtgg cgaatggggt
      961 ggccgttcga atgggcattt tgtatctcat gtctggagca taatcgtgac gatattgatt
     1021 caaggcgggc acattatcga cccggggcgc ttcaacgcgg tgggtgatct gttgattgat
     1081 caaggaaagg tcgttgctat tggaccaaac atcaaggctc cggcaggagc gacgatgatt
     1141 gtggccaagg gacgactcgt cgtgccggga tttgtcgatc tccacgtaca cttcagagag
     1201 cccgggttcg agtataagga aacgattcaa agcggagcgg cgtcggcggt cgcaggaggc
     1261 tttacgacgg tctgttgcat gcccaacacg aatcccgtca acgacaatca ggcgattacc
     1321 gagtttatat tggaacgtgc gcgggccgcc ggactggcca acgtgttgcc gatcggggcg
     1381 attaccaaag gttcggaggg aaaggaactg gctgagatcg gcgatttgcg ccgcgccggt
     1441 tgtgtggcga tctccgatga cgggaagccc gtcatgaaca gcctcgtgat gcgccgggcg
     1501 atggaatatg ccctggcgtt cgatgtgccc gtcgtcgacc attgcgaaga tttgcatctg
     1561 gccgaaggcg gctgcatgaa tgaaggcctc atctcgacag agttgggact tcccgggatt
     1621 cccgcagcgg cggaagacgt gatggtcgcg cggaatgtgt cgctggcaga attgacgggg
     1681 gcgcgattgc accttgccca tatcagtacg gtcgggtcgg tgcgtatggt gcgtgaagcg
     1741 aagtcgcggg gcatcaaggt cacggccgaa gcctgtcccc atcactttac gttgacggag
     1801 gaagtggttc ggggattcaa taccctcgca aaaatgaatc cgccgcttcg gacgtggaac
     1861 gacgtgcagg cgatcaaaga agggttgcgc gacggcacga tcgatgtcat cgcgactgat
     1921 catgcccccc atgccgcgca agagaagcag caggggttca ccgaagctcc attcggcatc
     1981 gtgggtcttg aaacagcgct tccgttaacc ttagcgctgg ttgaagaagg gattctcacg
     2041 ctggaagcag ccatcgagaa actcagcagc gcgccggcga agacctttag cctctcgaag
     2101 ggatcgttgg cagtcggtgc cgacgcggat gtagccgtca tcgaccctca tgtgcaatgg
     2161 gaggtggatc cggccaagtt tcgctcgaag agccgcaata cgccgttt
//
LOCUS       DATIFR010000215        17106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1103577, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000215 DATIFR010000000
VERSION     DATIFR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103577"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VLD60_12345"
     CDS             complement(<1..1270)
                     /locus_tag="VLD60_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB45798.1"
                     /translation="MMGVQLLVALLIRAHALQQPFRTLLTVVGVGLGVLASVAISAAN
                     VEVLRGFERAVLTVAGPATLEIVGHDIGLDETLITAVRHVPGVLVASPVIEEAIVALD
                     GAHRGESLQVYGLDLLEETGRRGFRLERGTSEEALNDLLAPESLYLGRRLGFEWNLAP
                     GSTIDVAVASQNVRLRVAGLIQSDGERASLWDRVAVMDLAAAQVLFGSVGRLDRIELV
                     TAADRTPEDVASAVRTVLPPHLIVQRPAQRTAQVEKMVGAFQLNLAVLSWVGLLVGMF
                     LVYNTMSFSVTQRRREIGIYRALGMTERHVAGLFLVEAGLFGLLGGLLGGLAGLWLAR
                     SLVLLVSRTISDLYVPLAAQGAAMTLDTHSVTALVQGVVVGMAVSMAGALGPSIDASR
                     TVTTKALAPGDYETTAQLRGRRYVWGSLALL"
     gene            1444..1710
                     /locus_tag="VLD60_12350"
     CDS             1444..1710
                     /locus_tag="VLD60_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45799.1"
                     /translation="MKSALRRKAEPAIEEPVDLELLTPRQRDILRLVSIGHTNREIAN
                     MLQISVRTVEVHRFNLMRRLNVRNVAQLLRRALQLGLLSKSFGK"
     gene            complement(1955..2884)
                     /locus_tag="VLD60_12355"
     CDS             complement(1955..2884)
                     /locus_tag="VLD60_12355"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HSB45800.1"
                     /translation="MMDLSTTIAGIKFPTCFMNASGALCVTRDELLALGRSNAGAIVT
                     KSMTVESRQGNPAPRYYGFPGGSINSMGLPNLGYKAYAEMIPELKKLGKPVIASVAGL
                     CEDDFPTIAQVINEAQPDLIEVNLSCPNIPGKPQIGYDAEASERLMKRVRNVITVPMG
                     VKLPPYFDPAHHKLMGDVIGRCGVDFLNLINSVGNGLVVDPVTETVVIKPKGGFGGLG
                     GTIIKPVALANVRAFWKHFKGRMPIIGTGGVMNGVDAFEHFLCGASAVQIGTVLVEEG
                     VEVFKRLEQELAACLDKKGYRSVEECRGKLKEL"
     gene            complement(2881..3579)
                     /locus_tag="VLD60_12360"
     CDS             complement(2881..3579)
                     /locus_tag="VLD60_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB45801.1"
                     /translation="MVTLKQVSKVYERGGDHICGLDRVSLHIEAGAFCTFVGPSGCGK
                     STLLNLIAGLDMPTSGEILLDGQSTARFTVNDWTRTRRQSIGIVFQAFHLVPGLTVEE
                     NVAFPLLLRGEDGLTVRKRVADVLGLVAMAHRSQHRPSELSGGEQQRVAIARAIVHRP
                     KIILADEPTGNLDSHHGAEIITLLRRLVQEFHQTVLLVTHSAAAAEAADYTWTMKDGQ
                     LVSRTAQQAVLTGA"
     gene            3702..4523
                     /locus_tag="VLD60_12365"
     CDS             3702..4523
                     /locus_tag="VLD60_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSB45802.1"
                     /translation="MLPTSADSQILGWYRRALRFLMPVECIGCDRPLTDDPVPFFCRE
                     CWDRIMPLAGSTCARCDQPFVSSAATSWTPDHQCQTCLERPPAYQRAWTLFPYLPPLQ
                     DAICSFKYRGKVGLAKPLASLMINALPRGIDADLILPVPLHPARLRAREFNQSLLLAD
                     QLARHLSRPVSSTHLVRVLSTDPQTTLSRRERLRNLRQAFAVRMPDEVVGRRLLLVDD
                     VFTTGTTLNECAKALLNAGAESVCALTLARTIDTSLVPDRILAEHATRFLSAIGI"
     gene            4527..4871
                     /locus_tag="VLD60_12370"
     CDS             4527..4871
                     /locus_tag="VLD60_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB45803.1"
                     /translation="MPLYEYRCLDCKQRSTILVLSLANQKPALCTHCQSARLERLMSR
                     FASPKSEEARLESLTDPNALAGLDENDPESMARFIKKMGDEMGEDLGDEMAESLESPE
                     SDLPESDGTDTL"
     gene            5009..5179
                     /locus_tag="VLD60_12375"
     CDS             5009..5179
                     /locus_tag="VLD60_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB45804.1"
                     /translation="MTAEETCRYLKITQRTLYRYLQSRQIPAFKLGKEWRFVRSDLEQ
                     WIRDRTRGPVAS"
     gene            5196..5636
                     /locus_tag="VLD60_12380"
     CDS             5196..5636
                     /locus_tag="VLD60_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45805.1"
                     /translation="MFAGEYLCKVDEKGRFIVPSPIREQVESEGQAVVFLKGPEESIL
                     IYSSREWEKVLERTKTTLDEEQSRLFMHFVVSEAGTSEIDKAGRILIPGKLRKLVPLD
                     EDQEIILVGLFHRMEIWNPSEWRRYIARSEDRYEQNMAKILNLL"
     gene            5827..6204
                     /locus_tag="VLD60_12385"
     CDS             5827..6204
                     /locus_tag="VLD60_12385"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HSB45806.1"
                     /translation="MSLILPVPPSINHQYATVNGRRLLSTAGRAYKAQVGQLVWLNLA
                     KSPARATLLAGLQSASLALSIRFYFTSALRRDVDGGLKIAQDALCEGLGINDNRIVET
                     HLYKHVDKANPRIEVRLTPTTRS"
     gene            complement(6248..7483)
                     /locus_tag="VLD60_12390"
     CDS             complement(6248..7483)
                     /locus_tag="VLD60_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB45807.1"
                     /translation="MSAFIWLTVMTALRILGRNRLRAGLTMLGIVIGVAAVIAMVGIG
                     EGAKRAVQAQIATMGTNVIIVFPGFTSASGVRGSQGTAITLTVSDALDLKKRVPLLVD
                     TGWSRRDVMQIVNGNRNWNGPVNGVSPSYLTIRDWSFSSGGTFTQTDLDSAARVALIG
                     QTIVDNLFDPGEEPVGAIIRLKNVPFRVIGVLSPKGQSAQGSDQDDVIFIPFTTAERK
                     VFGTSFIGSIGAMFASTAQSEDLPAAVEQMREVLRARHRLQGEQADDFTIKTQVDIGR
                     VQEGTSQTLTIMLFSIASVSLLVGGIGIMNILLVSVTERTREIGVRMAVGAKRRHIVM
                     QFLIEAMTLSLLGGAVGIVLGVVGAKLTTIVAGWPTVISSDVIVVAFFFSLAVGLFFG
                     LYPANKAARLNPIDALRYE"
     gene            complement(7484..8194)
                     /locus_tag="VLD60_12395"
     CDS             complement(7484..8194)
                     /locus_tag="VLD60_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB45808.1"
                     /translation="MIPLIECNDIWKIYRVGDMEVQALRGLSLTIEQGEFVAIMGSSG
                     SGKSTLMNILGCLDQPTKGFYRLNGVEVENLRPDQLAEIRNQQIGFVFQSFNLIPRTS
                     ALENAQLPLFYRGLSLKEQRAKAAAALQRVGLSGREHHYPTQLSGGQQQRVAIARALV
                     TAPSLLLADEPTGNLDTQSSREIMAILEGLNREGITVILVTHEVDVAAYAHREIVIKD
                     GQVLTDRPTKTRTQTVGV"
     gene            complement(8333..9598)
                     /locus_tag="VLD60_12400"
     CDS             complement(8333..9598)
                     /locus_tag="VLD60_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB45809.1"
                     /translation="MRRVVLIAAVLVVGLAIAGYVFFNGERKVPVRYRTAMIERGSII
                     SLVTATGTINPVVSVQVGTQVSGMIKSLHADFNSVVKAGDIVATIDPEPFKARRDQAA
                     SNLEMAKANVARARTDQAQRRRELERAKSLIDQQFVSQNDLDVAITNAQGADAQVNVV
                     MAQVKQAEAALNAADLDLKYTTIRSPVNGIVVARNIEVGQTVAASFATPNLFLIALDL
                     TKMQVDTNVSESDIGGISEGKEATFTVDAYPGVPFSGTIRQVRLAPINVQNVVTYNVV
                     VGVDNRDLRLKPGMTANVSIVVAQKDQVLKVPNAALRFVPPKSEQSEFRQPEGKPVKA
                     EGGARAIPAGGALARNIWKHTDNGELAPVVVQTGISDGSATEIVSEAVSEGDQVIVGI
                     DQSRGPKKSGDLPPGFGSGGQRRSRDRGL"
     gene            complement(9749..10456)
                     /locus_tag="VLD60_12405"
     CDS             complement(9749..10456)
                     /locus_tag="VLD60_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45810.1"
                     /translation="MKPVWMAVVIVAGAVSVVGCVSDRKYQEALAEAEAARTDLATTR
                     AQKSALEQQVKTLKELNVKFGNEAQAAHDELDRIQHGRDKERGSAEVRTKELEDRVKQ
                     LAAQNRAVRQEYEDAKRHNETLKSLVARYQKELKDQSRSSAGALAPSMSPAPAPSSPS
                     TSASPAPAGVPSAAGIMNVNKASSSEMVLLLGLSKDVADRIVSNRPYKVKGELVAKNV
                     VPKETFDMIKDRISVSQ"
     gene            10736..11614
                     /locus_tag="VLD60_12410"
     CDS             10736..11614
                     /locus_tag="VLD60_12410"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSB45811.1"
                     /translation="MNEWSPALAVILGIVEGLTEFLPVSSTGHLILAGHALGFTGDVA
                     ANAEISIQLGAILAVVAYEREKLRTLFSRAIGEQADLRRSLQRPTQTAWGETLRRSIA
                     AHPHLWFLLGLGAAFLPAGLVGFVTHGWIKAHLFSPQTVAITSIAGGLIILAVEARQA
                     PASVQQLEQVGLRNAVLIGLAQCASLIPGMSRSGSTIIGGLLVGLDRKIATEYSFFLA
                     LPTLIIATCYQMWKSRGLFRQEDYVTLGIGLLVSFVVAWIVIAAFLTFVKRHTLRPFA
                     YYRILMGIVVLYIFGL"
     gene            11696..11812
                     /locus_tag="VLD60_12415"
     CDS             11696..11812
                     /locus_tag="VLD60_12415"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HSB45812.1"
                     /translation="MHFDVMTTDEATALKLAQQYVVSLGEHAVTVTLKECPF"
     gene            complement(11902..12426)
                     /gene="lspA"
                     /locus_tag="VLD60_12420"
     CDS             complement(11902..12426)
                     /gene="lspA"
                     /locus_tag="VLD60_12420"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSB45813.1"
                     /translation="MRHSVRYLLLALIGVTVVVVDQVTKLWIVQSMRLHESIPIVPNL
                     FSLTYIRNPGAAFGLLAGSSNAFRMAFFAVTSLFALALLGTILFRLPEKDWIGQVSIA
                     GILGGAIGNLIDRLRFGEVIDFLDVYVESYHWPAFNVADSAISVGVVCLIIHFAFERK
                     ETTLPAPAPPSSSV"
     gene            complement(12423..15236)
                     /gene="ileS"
                     /locus_tag="VLD60_12425"
     CDS             complement(12423..15236)
                     /gene="ileS"
                     /locus_tag="VLD60_12425"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSB45814.1"
                     /translation="MDYKSTLNLPKTDFPMKANLPQREPELLAWWEQERLYEKIQEAG
                     KGRPRYLLHDGPPYANGRIHIGHALNKILKDIVVKSKTMAGFQAPYVPGWDCHGLPIE
                     HQVLKDLGEKKKTLDAMAIRKLCREYAEKFFKIQREEFQRLGVLGDWQQPYLTMNSAY
                     EATIVREFGKFVERGGVYKGLKPVLWCTQDQTALAEAEVEYEDHTSPSIYVKFPVVTS
                     PKALSARFPGVSLPADIKLVSVVIWTTTPWTLPANQAVCLHADIDYAFVQVGQEVLII
                     AHKLLDNVAKACKLEQATVLAVKKGGEGFEGLETQRPLTTGLSPVLLGDFVTLEQGTG
                     CVHIAPGHGMEDYILVLEHNAKASPGERLEILAPVDNAGRFTDVVKEFAGLHVFKANP
                     KIVEFLQDNGRLLGHGSLDHSYPHCWRCKSPVIFRATEQWFVSMETNELRKDALAEIG
                     RVRWIPAYGRDRINGMIENRPDWCLSRQRVWGVPIPGFTCIGCRHVLADPVIIDHIAT
                     QMEAKGADVWFERSASELLPHGTTCPKCGKAEFEKEHDILDVWFESGVSYAAVLKPRK
                     WWPADLYLEGSDQHRGWFHSALLAGVVTDRRAPYHAVLTHGFVLDGQGKKMSKSAGNV
                     VAPQDVIKQSGAEILRLWVSAQDYREDLRISPEILNHLIEAYRKIRNTCRFLLSNLYD
                     FNPTKHRVPYEQLPELDRWALMRLEELKGRVRQAYDDFEFHAIYHALNNFCSVDLSAV
                     YLDILKDRLYTFRADSPLRRGSQTVLYDIIVAMTQLMAPVLSFTAEEIWRSLAAQMGS
                     AFGSTSVHLSGFPEAHPEWQDAKLAQRWEQLLDYRTQVQGVLEGGRRDKVIGSSLEAA
                     VELRASPGDHAFLKSYERDLPTVFIVSQVKLTESGDAKGPLAIVAAKSSFGKCERCWN
                     YREAVGKHATHPTLCDRCVEAVQ"
     gene            complement(15441..16532)
                     /gene="nadA"
                     /locus_tag="VLD60_12430"
     CDS             complement(15441..16532)
                     /gene="nadA"
                     /locus_tag="VLD60_12430"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSB45815.1"
                     /translation="MTLPKPITDYQQLSAEELFGRTAEAKRILGDQVMILGHNYQRDE
                     VIEHADFRGDSLLLSKLAAERSERPYVVFCGVHFMAETADILSRTNQTVILPDMAAGC
                     SMADMAAIEQVDQCWETLGRILPVEETVMPAVYVNSAAVLKAFCGEHGGITCTSSNAK
                     AVIEWCWARREKILFFPDEHLGRNTANKMGLPREQMIVWDPYQPNGGNTREAINRATL
                     ILWKGHCSVHQMFQPVHVDNFRKQYPDGTVIVHPECHEDVVNKADLSGSTEFIIKTVT
                     AAPAGTVWAVGTELNLVNRLKRDLTDKKVFFLSSTVCQCATMFRIDGVHLCWAMENLA
                     DGHVVNRIVVPEDDKRWARIALDRMMAVS"
     regulatory      16711..16813
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         3641 a         5300 c         4596 g         3569 t
ORIGIN      
        1 tcagcaatgc gagactcccc catacatatc gccgccctcg gagttgcgcg gtcgtttcat
       61 aatctcctgg cgcgagggct ttggtcgtca cggttcggct cgcatcgatg ctgggaccaa
      121 gcgcacccgc catcgacacc gccatcccga cgacaacccc ttgaacgaga gccgtgacgc
      181 tgtgcgtatc taacgtcatc gcggctcctt gtgcggccag aggcacgtaa agatcgctga
      241 tcgtacggct gacgagcagc actaaactcc gagccagcca aaggcccgca aggccgccaa
      301 gcaatcctcc gagcaagcca aacaatccgg cttcgacgag aaacaacccg gccacatgcc
      361 gttccgtcat acccagcgca cggtagatgc cgatctcccg gcgtcgttgg gtcacggaaa
      421 aactcatcgt gttgtagacc aagaacatcc cgaccagaag tccgacccag ctcaggaccg
      481 ccaggttcag ctgaaaggca ccgaccatct tctcgacttg cgcggtccgc tgcgccggtc
      541 gttgcacaat aagatgcggc gggagaactg tgcgcacggc cgaagcgacg tcttcgggcg
      601 ttcgatcagc ggccgtcacc agctcgatgc gatccagccg gccgaccgac ccgaagagga
      661 cttgcgctgc agcaagatcc atgaccgcca cacgatccca cagcgacgcc cgttccccat
      721 cactctgaat caggcctgcc acccgcagcc gcacattctg agaagccact gcgacatcaa
      781 tcgtactccc cggcgcgaga ttccattcga aacccagccg acgtccaaga taaagacttt
      841 cgggtgccag cagatcgttc agcgcctctt ctgaagtacc tcgttccaac ctgaaaccac
      901 gacggccggt ctcctcgagc agatcgaggc cgtagacttg gaggctttcg ccacgatgcg
      961 ctccgtcgag tgccacaatc gcttcttcaa tcacggggga agccacgagc acgcccggaa
     1021 catgccgaac agcagtgatg agggtttcgt cgagaccaat atcatggccg acaatttcca
     1081 gcgtggcggg accggcaacc gtcaagacgg ctcgttcgaa cccccgaagc acttcgacat
     1141 tcgcggcaga gatggccacc gacgccaata cgccgaggcc cacaccgaca acggtaagaa
     1201 gggtacgaaa cggttgttga agagcatggg cacggatcaa caaggcgacg aggagctgaa
     1261 cacccatcat cgaacctccg ccttcgcgct gaacggcgta gcatgaacac ctagccaacc
     1321 tgtcatttca tcaatatcca tgcgggcgaa agtgactctg cacaaattag tagtttcgtt
     1381 tacagagttc cacacctttg ctagactcgg ttcagttaat gctggcaatc gagggtgggg
     1441 gaaatgaaaa gcgcactacg tcgaaaagcc gaaccggcca ttgaagagcc cgtcgatctc
     1501 gaacttctga caccgcgaca gcgcgacatc cttcgtctgg tgtcgatcgg gcatacgaac
     1561 cgcgaaatcg cgaacatgct gcagatcagc gtccggaccg ttgaagtaca ccgcttcaat
     1621 ttgatgcgcc gcttgaacgt gcggaacgtc gcgcagctgc taaggcgagc ccttcagctc
     1681 ggactcctct ccaaatcctt cggcaagtaa ccataccagg ctgttgaaaa agtccgccag
     1741 ctgcgttctc gcatcgctca ggcgctcagc gtatacaaaa gccgacttga ggcaaagtaa
     1801 ctatccgctc ccatgtgatc gaagcgagcg gctcaagcga agctcggtct gtacctcctc
     1861 gccccttcac tcaccgcggc cttgcctgcg gagcggtgcg tctcagcgca ccggggttgg
     1921 gcgggtgaga atcgagacct ttttgaaccg cctgctataa ctccttcagc ttccctcggc
     1981 attcttccac cgaccggtag cctttcttat cgaggcaagc ggcgagttcc tgctccagtc
     2041 gcttgaacac ctcgacgcct tcctcaacca gcacggtccc aatctgcacg gcagaggcgc
     2101 cgcagagaaa atgctcgaac gcatcgacgc cgttcatcac gccgcctgtg ccgatgatcg
     2161 gcatgcgccc tttgaagtgc ttccaaaaag cccgcacgtt ggccaacgca accggtttaa
     2221 tgatcgtccc gccgaggccg ccgaaaccac ccttcggctt gatcaccacc gtctcggtta
     2281 caggatccac caccaagccg ttgccgacgg aattgatcag gttgaggaaa tccaccccgc
     2341 atcgtccgat cacgtcgccc atgagcttgt gatgcgccgg atcgaagtag ggcggcagct
     2401 tcacgcccat cggcaccgtg atcacgttgc gcacccgctt catcaagcgc tcggaggcct
     2461 ccgcatcgta gccgatttgc ggtttccccg gaatgttagg gcacgacaga ttcacttcga
     2521 tcaaatcggg ctgtgcctca ttgatcactt gggcgatcgt cgggaaatcg tcttcacaca
     2581 gacccgcgac actggcgatc accggctttc ccagcttttt caattcggga atcatttcgg
     2641 cataggcctt gtacccgagg ttgggcaacc ccatggaatt gatcgatcca ccaggaaatc
     2701 cataatagcg cggcgcaggg ttaccttgac gtgactcaac cgtcatcgac ttggtcacaa
     2761 tcgcgccggc attggaacgg cccagtgcca gaagttcatc ccgcgtcaca cagagggccc
     2821 cggatgcgtt catgaagcag gtaggaaact tgattccggc gatggtggtt gaaaggtcca
     2881 tcatgctcct gtcaggaccg cctgctgagc cgttcgcgag accagttgcc catctttcat
     2941 cgtccacgtg tagtcggctg cttccgcggc cgccgcgcta tgcgtcacca gcagtactgt
     3001 ctgatggaac tcctgcacca gccggcgcag cagcgtgatg atctcggctc catggtggga
     3061 atccaaattc ccggtcggct cgtcggccaa aatgatcttc ggccgatgca caatcgcccg
     3121 ggcgatggcc acgcgttgct gctcgcctcc cgacaactcg ctaggccgat gctgactgcg
     3181 atgggccatg gcaaccagcc ctagcacgtc cgccactcgc tttcgaaccg tcagaccgtc
     3241 ctcgcctcgc aacaacaacg ggaaggccac attttcttcc accgtcaatc cgggaactag
     3301 atgaaaggct tgaaacacga taccgatcga ctgccgcctg gttcttgtcc agtcgttgac
     3361 ggtaaatcgg gccgtggact gcccgtccag caggatctcc cctgatgtcg gcatgtcgag
     3421 cccggcgatc agattgagca acgtactctt gccgcaccca ctgggcccga cgaaggtgca
     3481 gaaggcacct gcttcaatgt ggagactcac tcgatcaagg ccacagatat ggtctccccc
     3541 ccgctcatag accttggaca cctgtttgag cgtcaccata atggggagcg tcctccttcg
     3601 atcgatctcg ctttcgtata tcacaaccat ttacagagcg acaaccttga cagaagacga
     3661 gaagcgcgat agagactgcc ggaaacctac ggagtatgtc gatgcttccg acaagcgcgg
     3721 acagccagat tctcggatgg taccggcgag cccttcgatt tctgatgccg gtggaatgca
     3781 tcggctgcga tcgaccgttg acagacgacc cagtgccatt cttctgccgt gaatgctggg
     3841 accgcatcat gcctctcgcc ggatcaactt gtgcccgctg tgatcagccg ttcgtttcgt
     3901 cggccgccac atcctggaca ccagatcacc aatgtcagac ctgtttggaa cgtccgccgg
     3961 cgtaccagcg ggcctggacc ctcttccctt atctcccccc attgcaagac gccatctgtt
     4021 ccttcaaata tcgcgggaaa gtcggtttgg caaagcctct cgcctcgctt atgatcaacg
     4081 cattaccgag agggatcgac gccgatttga tcctgccggt gcctttgcat cctgccagac
     4141 tccgagcgcg cgagtttaat caatcattgt tgctcgcaga ccagctcgcc cgccatcttt
     4201 cacgtcccgt ctcgtcaacc catcttgtcc gcgtgttgtc gacagatcct caaacaacgc
     4261 tctcgcggcg tgagcgattg cgaaatctcc gacaggcctt tgcggtccgt atgccagacg
     4321 aggtcgtcgg gcgccggctc cttctcgttg atgacgtctt tacgaccggc acgactctca
     4381 atgagtgtgc caaggctctg ctgaatgccg gcgctgagtc tgtgtgcgcg ctgacgctgg
     4441 cgcgcacgat cgatacgagt ctcgttccgg accggatcct cgccgagcac gcaacaaggt
     4501 ttctgagcgc aataggaata taggcgatgc cgttgtacga ataccgatgc ctcgactgca
     4561 aacaacgcag cacaatcctt gtcctcagcc tcgccaatca aaaaccagcg ctgtgcaccc
     4621 attgccagag cgctcgactg gagcgattga tgtctcgatt tgcttcaccc aagtcggagg
     4681 aggcaaggct ggaatcgtta accgacccga acgcgcttgc cggactcgac gaaaacgatc
     4741 cggaaagcat ggctcgattc ataaaaaaga tgggagatga gatgggcgag gatctgggcg
     4801 acgagatggc cgaatccttg gagtcccctg aatcagacct gccggaatcg gacggaactg
     4861 acaccttgtg acaggcattg tcatatattt tcacttgaca gatttgcttt gttttcttac
     4921 cctgagggca gtttccggag ccttatgagg cacatgaaca acaattggtt acggtttcct
     4981 catggccaaa gagcccaaga gcgagctgat gacagcggag gagacgtgcc gctacttgaa
     5041 gatcacccag cggacgctct atcgctacct ccagagccgg cagattcccg ccttcaaact
     5101 tgggaaagaa tggcgattcg tacgttcgga tctcgagcag tggatccgcg accggaccag
     5161 aggccctgtc gcgtcgtgaa ggagacctgg cactgatgtt tgccggcgaa tacctctgca
     5221 aagtggatga gaaggggcgc tttattgtcc cctcacccat tcgtgaacag gtcgagtccg
     5281 agggacaagc tgttgtcttc ctcaaaggcc ctgaagaatc tatcctcatc tactcctcga
     5341 gagagtggga gaaggttctg gaacggacga aaactacgct ggacgaggaa cagagccggc
     5401 tgttcatgca tttcgtggtc tcggaagcgg ggacgtcgga aatcgataag gcggggcgca
     5461 ttctgattcc ggggaagcta cggaaactcg tcccgcttga tgaggatcag gagattattc
     5521 tcgtcggtct cttccatcgc atggaaattt ggaatcccag cgagtggcgc cgctatatcg
     5581 ctcgttccga agaccggtac gagcagaata tggcgaagat cctaaatctt ctctaaactg
     5641 gcgcgcatgc ctacctatcg gcgccagatc cgaatccctc tttccaaact cccagtccgc
     5701 ctcacttcct atcgaacgat cgattccgcc gccggctctc ggcgcccatc cactcgaatc
     5761 gcatctgcgc ctcggaggct ccccgcgtcc gcaacgccgg cgcaaccagc ggcatcagat
     5821 gttgccgtgt ctttgatcct gcccgtccct cccagcatca atcatcaata tgcgacagtg
     5881 aacgggcggc gcctgctttc caccgcaggc cgcgcctaca aagcccaagt gggccagctc
     5941 gtctggctga acctggcaaa atccccggct cgcgctacgc tgttggcggg tttacagtct
     6001 gcctcgctcg ccttgtccat ccgcttctac ttcacgtcgg ccctgcgacg cgacgtcgac
     6061 ggcggactca aaattgccca agacgccctg tgcgaaggac tcggcatcaa cgacaaccgt
     6121 attgtcgaaa cccatctcta caaacatgtc gacaaagcca accctcggat cgaagtcagg
     6181 ctgaccccga ccacccgttc ttagaacatc tcgtcgggcg ttcaaacact ctcttacggt
     6241 actgatgtta ttcgtaccgc agcgcgtcga taggattgag tctggcggct ttgttggcgg
     6301 gatacaatcc gaagaaaagt ccaaccgcaa gcgagaagaa aaaggcgacg acgatcacgt
     6361 cactcgagat aaccgtcggc cagccggcca cgatcgtggt gagttttgcg ccgacaacac
     6421 ccagcacaat ccccaccgca ccccctaaga ggctcaacgt catggcttcg atgagaaatt
     6481 gcatgacgat atgacgacgc ttggctccaa cagccatgcg cacgccgatc tccctggtgc
     6541 gctcggtcac cgacacaagc agaatgttca tgatgccgat tccgccgacc agcaaagata
     6601 cggacgcgat ggagaacagc atgatcgtca gggtttggct cgttccttct tgcaccctgc
     6661 cgatgtccac ctgcgtcttg atggtgaaat catccgcctg ctcgccctgc aaccggtgtc
     6721 gcgcacgaag cacctcacgc atctgctcga cggcagcagg caggtcttca gactgagcag
     6781 ttgaggcaaa catcgcccca atggatccga taaatgacgt accgaacacc tttcgctctg
     6841 ccgtggtaaa aggaataaag atcacatcgt cctgatcgga accttgcgcg gactgcccct
     6901 tcggcgagag cacgccgatc acgcggaacg gcacgttctt gaggcggatg atcgcgccca
     6961 ccggctcctc gcccggatcg aacagattgt ccacgatcgt ttggccgatc agcgcgacac
     7021 gggctgcgct gtcgagatcc gtttgcgtga acgtcccccc gctgctgaat gaccaatccc
     7081 ggatcgtaag ataactgggc gacacgccgt tgaccggtcc attccaattc cgattcccgt
     7141 tcacaatttg catgacatca cgtcgagacc atcccgtgtc caccaggaga gggacccgtt
     7201 ttttcaggtc caaggcatcc gacactgtga gggtgatggc tgttccctgg cttccccgaa
     7261 caccgcttgc tgaggtgaat ccggggaaca cgatgatcac gttcgtcccc atcgtggcaa
     7321 tctgggcttg taccgcccgc ttggcgcctt ccccgatccc taccattgca atcacggctg
     7381 ccacgccgat gacaatgccc agcatcgtca gcccggcgcg caggcgattg cgccccagga
     7441 tgcgcaatgc cgtcatcacc gtgagccaga tgaaggccga catttagacc ccgaccgtct
     7501 gcgtgcgggt tttggttggt cgatcggtca acacttgtcc gtccttgatg acaatctcgc
     7561 ggtgtgcata ggccgcaaca tccacttcat gcgtcaccaa aatgacggta atcccctccc
     7621 ggttcagacc ttccaggatg gccataattt cccgactcga ttgcgtatcc aaatttccgg
     7681 tcggttcatc ggccaagagg agcgagggcg cggtgaccag tgcccgtgcg attgccacac
     7741 gctgctgttg cccgccggac agttgcgtcg ggtaatggtg ttctctcccg ctcagcccca
     7801 cgcgctgaag cgcggcggca gccttcgccc gttgctcttt cagcgaaagt ccccgataga
     7861 acagcggtaa ttgtgcgttt tcgagagcac tggtgcgagg gatcaaattg aaactctgaa
     7921 agacaaaccc gatttgctgg ttacgaattt ctgccagttg atcgggcctg aggttctcga
     7981 cctcgactcc attcagccga tagaagccct tcgtcggttg atccagacat cccagaatat
     8041 tcatgagtgt ggacttgccg gagccggatg atcccataat cgcgacgaac tcaccctgct
     8101 caatggtgag gctgagtccg cgcaacgcct gcacctccat gtcgccgaca cgataaatct
     8161 tccagatgtc gttgcactca atcagcggaa tcatgggagg cattctcgaa acatgtggcg
     8221 cggaacgtca caggatcctg ccgaagagac ttggcgaaaa agtagaacga ttgttccgga
     8281 gagacactcg gacagcaatg gacgagtcac caaacgcgct tcacccttta ctctataggc
     8341 cgcggtcacg cgaccgccgt tgcccgccag atccaaaccc tggcggcaga tccccgctct
     8401 tctttgggcc cctggactgg tcaatcccca cgatcacctg atcgccttca gagaccgctt
     8461 cagacacaat ctctgtcgcc gatccgtcgg atattcctgt ctgcacgacc actggagcga
     8521 gttcaccatt atcggtgtgc ttccagatgt ttcgagccaa ggctccgccg gcaggtatgg
     8581 ctcgagcgcc tccctcggct ttgaccggct ttccctccgg ctggcgaaac tcgctctgct
     8641 cggatttcgg tggcacaaac cggagcgcgg cgttgggaac tttaagcacc tggtccttct
     8701 gcgccacgac gatggacaca ttcgccgtca tgccgggttt caagcggaga tccctattgt
     8761 cgacaccgac cacgacattg tatgtcacga cattctgaac attgatcgga gcgagacgca
     8821 cctggcggat ggtccctgaa aacgggactc cggggtaggc atcgaccgtg aacgtcgcct
     8881 ctttcccctc ggaaattccg ccgatgtccg actcgctgac gttggtatcc acctgcattt
     8941 tggtcaggtc gagcgcgatc agaaagagat tgggcgtcgc aaagcttgcc gcgaccgtct
     9001 ggccgacttc gatgtttctg gccaccacga tgccgttgac cggcgagcgg atagtcgtat
     9061 acttgagatc caaatcggct gcattcagcg cggcttcagc ttgcttcacc tgcgccatga
     9121 ccacattgac ctgtgcgtcc gccccctgcg cgttggtaat cgccacatcc agatcgttct
     9181 gcgaaacgaa ttgttggtcg atcagcgatt tggcccgttc caactcgcga cgtcgctggg
     9241 cctggtcggt cctggccctt gcgacattgg ccttcgccat ttcaagattg ctggcggctt
     9301 gatcccgacg ggccttgaac ggttccgggt cgatagtggc gacgatatcc ccggctttga
     9361 caaccgagtt aaaatcggcg tgcaaactct tgatcatccc ggagacttgt gtgcctactt
     9421 gaacggatac gacagggtta atcgtgccgg tggccgtgac aagggaaatg atcgagccgc
     9481 gctcgatcat ggcggtgcgg tagcgaacag gtactttccg ctcaccgttg aaaaagacat
     9541 agccggcgat cgccagtccg acgaccaaca ccgcggctat caggacgaca cgacgcatga
     9601 atccctctcg gcatggtcta cagcggctct attctagcaa gaagaggcag gggaatcatc
     9661 tattgcggcg ttctctaaaa caccacgggc cgtccattgt gcaagtggac ggcccgtggc
     9721 atcgtcaaac cgctacagcg tctgccgact attggctgac gctgatccga tctttgatca
     9781 tgtcgaatgt ctctttcggc accacgttct ttgccaccaa ctcacccttc actttgtacg
     9841 ggcggttgga aacgatccga tccgccacat ctttgctcag tcccagcaac agcaccattt
     9901 cgctggaaga ggccttgttc acgttcatga taccagcggc gcttggcact ccggccggcg
     9961 cgggagacgc ggatgtcgac ggtgaagacg gagccggcgc gggggacatt gacggggcca
    10021 gagctccggc gctcgagcgg gattgatcct tcagttcttt ttgatagcgt gcaacgaggg
    10081 acttcaacgt ctcgttatga cgcttggcat cctcatactc ctgtcgcacc gcacgattct
    10141 gcgcagccag ctgcttgacg cgatcttcca attccttcgt acggacttcg gcactgccgc
    10201 gctccttgtc tcttccatgc tgaatacgat cgagctcatc atgggcagcc tgcgcctcgt
    10261 tcccgaattt cacattcaac tctttcagtg ttttgacctg ctgttccaac gcgcttttct
    10321 gtgcgcgggt tgtggccagg tcggttctag ccgcttccgc ttcggctaac gcctcttgat
    10381 acttccggtc cgacacgcag cccaccactg agactgctcc cgccacgatt accacggcca
    10441 tccacactgg cttcatgaga ccctcccctt ccctcagaca ttcttctcac cgcatcgtgc
    10501 tcgtaccatc agatgcggca tgggcgtcaa cgaatggaca cccacctcta cgtataagac
    10561 tctgtgtatg ccaagcacga tactttgtca acaggtttgc gggcaggatg gcgatccggc
    10621 ctgcatgcaa gcactcgaat catttcttca ctctgcttga ctgatagcga ccactttgtg
    10681 attatgcgcg agaccctctc acatcacact cgcacacgaa ttcgaaggtc ccagtatgaa
    10741 cgaatggagc ccggcccttg cagttattct cggcatcgtc gagggactga cggaattctt
    10801 gcccgtctcg tcgaccggcc atctcatttt ggcaggccac gccttggggt ttaccgggga
    10861 cgtggcggcc aacgccgaga tttcgatcca actgggtgcc attctcgccg tcgtcgccta
    10921 cgagcgggag aaacttcgga ctttgttctc gcgcgccatc ggtgagcaag ctgacctgcg
    10981 gcgatccttg caacgcccca cccagacagc ctggggcgag acccttcgtc gatccatcgc
    11041 ggcccatcca catctctggt tcttgcttgg gctgggagcc gcattcctgc cggccggcct
    11101 ggtgggattc gtcacgcacg gctggatcaa ggcccattta ttcagcccgc aaaccgtcgc
    11161 catcacctcc atcgccggcg ggctgatcat cctggccgtg gaggctcgcc aggcaccagc
    11221 cagcgttcag cagctggaac aggtcgggct tcgcaacgcc gtgctcatcg gactggcgca
    11281 atgcgcctcc ctgattcccg gcatgtcgcg atcaggctcg acgatcatcg gaggtctgct
    11341 tgtcggactc gaccggaaaa ttgcgaccga atactccttc ttccttgcgc tcccgaccct
    11401 tattattgcg acgtgctatc agatgtggaa atcgcgcgga ttattccggc aggaggatta
    11461 tgtgacactc ggcatcggcc tgctggtttc gttcgtcgtc gcctggatcg tcattgctgc
    11521 ctttctcacg ttcgtgaaac gtcatacgct gcgcccattt gcctattacc gcatcctcat
    11581 gggcatcgtg gtgctctaca ttttcgggct ctagctcggg aaatcatgat ggccgccgca
    11641 ccgtccgata ccgtgtacgt attcgataca tgggccagga gggaagaagg ggctcttgca
    11701 tttcgacgtg atgacgacgg atgaagctac cgcgctgaag ctggcgcaac agtacgtggt
    11761 tagcttaggc gagcacgccg tgacggtcac gctcaaagaa tgcccgttct gacacagcga
    11821 gccgcggtga tgtttcccgg cgaacagcag aaacaattcc gcgagcaggg cggatttatc
    11881 cttccactgc ctgcataaga gttaaacaga agacgacggc ggcgcgggcg ccggaagcgt
    11941 cgtttctttc cgctcaaacg caaagtgaat gatcagacac acgaccccga cgctgatcgc
    12001 cgagtcagcc acgttgaacg ccggccaatg gtaggactct acatagacgt cgagaaaatc
    12061 gatcacttca ccgaatcgga ggcggtcgat taaatttccg atcgctccgc ccagaatccc
    12121 cgcgatactt acctgcccga tccaatcctt ctccggcaac cgaaacaaaa ttgtccccag
    12181 cagcgccaag gcaaagagcg aagtgacggc gaaaaacgcc atccggaagg cgttgctgct
    12241 gcccgccagc aggccgaacg ccgcaccggg attccgaata taggtcaggc tgaacagatt
    12301 cggaacgatg ggaatcgatt catgcagccg catcgactgg acgatccaaa gcttcgtgac
    12361 ttggtcgaca acgaccacgg tgacaccgat caaggcgagc agcagatagc gaacactgtg
    12421 cctcattgca cggcctctac acagcggtcg cagagagtgg gatgggtcgc atgcttccct
    12481 actgcttcac ggtagttcca acaccgttca cattttccga acgacgattt ggccgccacg
    12541 atcgccaacg ggccttttgc gtcgccgctc tcggtcaact tcacctgcga cacgatgaag
    12601 acggtcggaa ggtcccgctc ataggatttc agaaacgcat ggtcacctgg gctggcgcgc
    12661 agttccaccg ccgcttccag cgaggacccg atcaccttgt cgcggcggcc tccttccagg
    12721 accccctgca cctgtgtccg atagtccaag agctgttccc atcgctgagc cagcttcgca
    12781 tcctgccatt cgggatgggc ctccggaaac ccgctcaagt ggacactcgt ggaaccgaag
    12841 gcgctcccca tctgcgccgc cagcgaccgc caaatctcct cggccgtaaa gctgagcacc
    12901 ggcgccatca gctgggtcat cgccacgatg atatcgtaca gcaccgtctg cgaaccgcga
    12961 cgcaacggtg aatcggcgcg gaacgtgtag agccggtctt tgaggatgtc taaatacacc
    13021 gcgctcaaat ccacagaaca aaaattattc agcgcgtgat agatcgcatg aaattcgaaa
    13081 tcgtcgtagg cttggcggac tcgccccttt agctcttcca atcgcatcag cgcccaacga
    13141 tcgagctcgg gcaactgctc gtagggaacg cgatgcttcg tcgggttgaa atcgtacaga
    13201 ttgctcaaga ggaaccggca ggtattgcgg atcttccgat aggcctcgat caaatggttc
    13261 aaaatctccg gcgagatgcg caggtcctct cgatagtctt gcgccgacac ccacaggcgc
    13321 agaatttccg caccggattg tttgataacg tcctgcgggg ccacgacgtt acccgccgac
    13381 ttggacatct tcttgccttg cccgtccaag acaaatccat gcgtgagcac agcgtgatag
    13441 ggagcgcggc gatcggtcac cactcctgcc agtaatgcac tgtgaaacca gcctcgatgc
    13501 tggtccgacc cctccagata gagatccgcc ggccaccatt tgcgcggctt gagcacagcg
    13561 gcataactga cgccggactc gaaccagacg tcaagaatgt cgtgctcttt ttcgaattcg
    13621 gctttcccgc atttcggaca ggtcgtaccg tgcggtaaca gttccgatgc cgaccgctcg
    13681 aaccacacat cggccccctt ggcttccatc tgggttgcaa tgtggtcaat aattaccgga
    13741 tccgccagca catgccggca gccgatacag gtaaagcccg gaatggggac gccccacaca
    13801 cgctgtcgcg agagacacca atcgggacga ttctcgatca taccgttgat acggtcgcgg
    13861 ccataggccg gtatccagcg gacccgcccg atctccgcga gcgcgtcttt gcgtagctca
    13921 ttcgtttcca tcgagacaaa ccactgttcg gtcgcacgaa aaatgaccgg actcttgcaa
    13981 cgccagcaat gaggatagga gtgatcgagc gagccgtgac cgagcaagcg tccattgtcc
    14041 tgtaggaatt ccacaatctt cggattcgcc ttgaacacat gcagcccggc aaactctttc
    14101 acgacgtcgg taaaccgccc cgcattgtcg accggcgcaa gaatttccaa ccgctcgccc
    14161 ggcgacgctt tggcattgtg ctcaaggacg aggatgtagt cttccatgcc gtgtcccggt
    14221 gcgatgtgga cgcagcccgt tccctgctcc aatgtcacaa agtctccgag caacaccgga
    14281 gacaatcctg tcgtgagggg ccgttgggtc tcaagacctt cgaagccctc cccgcccttt
    14341 ttcaccgcga ggacagtggc ctgctccagc ttgcaggcct tggcaacgtt gtcgagcaat
    14401 ttgtgggcga tgatcagcac ttcctgccca acctgcacga acgcatagtc gatgtccgcg
    14461 tgaaggcaga ccgcttgatt ggcgggcaac gtccatggag tggtcgtcca gatgacaacg
    14521 gagaccagct tgatgtctgc agggagtgaa acaccgggaa acctggcact gagcgccttg
    14581 ggtgacgtca ccaccggaaa cttcacatag atcgacggcg aggtgtggtc ctcatattcg
    14641 acttcagcct cggccaaggc ggtctggtct tgcgtgcacc aaaggaccgg cttgagccct
    14701 ttgtacaccc cgcctcgctc aacgaatttg ccgaactccc gcacgatcgt tgcttcgtat
    14761 gcggagttca tcgtcagata gggctgttgc caatctccca gcacaccgag ccgctgaaac
    14821 tcctcgcgct gaatcttgaa aaatttttcc gcatactcgc gacagagctt gcggatagcc
    14881 atcgcgtcga gcgttttctt tttctcgccg agatccttca agacctggtg ctcgatcggc
    14941 aagccatgac aatcccaacc aggcacgtag ggggcttgga atccggccat tgtcttcgac
    15001 ttcacgacga tatctttgag aatcttgttc agcgcatgac cgatgtggat gcggccgttc
    15061 gcgtacggag gaccgtcgtg cagcaggtac cgtggacggc ccttgcccgc ttcctgaatt
    15121 ttctcgtaca gccgctcctg ctcccaccaa gccagcaact ccggctcccg ctgcggcaag
    15181 tttgctttca tcgggaaatc ggtctttgga agattgagcg tcgatttata gtccatggac
    15241 gacggcctcg cgaaccaatg aattaacggg tgaatatacc ggaatgacgg gcgaggtact
    15301 agtccttagc cttttgcggg atcgtcaaaa acgacttccg gcaaggccgc agcgagcgag
    15361 agcccgagtc gtacggtttg atgtacgttg agggtttgag cgacgcgaga acgacgctgg
    15421 aggcgttttt cacgatctcg ttaactcacg gccatcatgc gatcgagcgc aatcctggcc
    15481 catcgcttgt cgtcttcagg aacaacgatg cggttcacga catggccgtc ggcgaggttc
    15541 tccatagccc aacagaggtg gacgccgtcg atgcggaaca tcgtggcgca ttggcagacg
    15601 gtggatgata ggaagaagac tttcttgtcg gtcaagtcgc gtttcaaacg attgaccaga
    15661 ttcagttccg ttccgaccgc ccaaaccgtt ccagcaggag ccgccgtcac ggtcttaatg
    15721 atgaactcgg tcgagccaga cagatccgcc ttgttcacga catcctcatg gcactcagga
    15781 tggacgatga ctgtgccgtc cggatactgt ttgcggaagt tgtcgacatg aaccggctgg
    15841 aacatctgat gcacgctgca gtggcccttc caaaggatca gcgtggctcg gttgatcgcc
    15901 tcgcgggtat tgccgccgtt cggctgatag ggatcccaca cgatcatctg ctcgcggggc
    15961 agtcccattt tgtttgccgt attgcggccc aagtgttcgt ccgggaagaa gagaattttc
    16021 tcgcgccgag cccagcacca ttcaatcacg gctttcgcat tcgatgaggt acaggtgatc
    16081 ccgccatgct cgccgcaaaa cgccttgagg accgccgccg aattgacgta gaccgccggc
    16141 atcaccgttt cctccaccgg cagtatgcgt ccgagtgtct cccagcactg atcgacttgc
    16201 tcgattgcag ccatgtcggc catcgagcaa ccggctgcca tgtccggaag gatgacggtc
    16261 tgattcgtcc ggctgagaat gtccgccgtc tccgccatga aatgcacgcc gcagaagaca
    16321 acatagggac gctctgagcg ctcagcggcc aacttcgaga gcaacagcga atccccgcgg
    16381 aaatccgcat gctcgatgac ttcatcccgt tgataattat ggccaagtat catgacctgg
    16441 tcgcccagaa ttcgcttggc ctcggcggtt cggccgaaga gttcctcggc agacagctgc
    16501 tggtagtcag tgatcggttt aggaagtgtc aacagtccct tcacaggtcc ctcgggtaaa
    16561 tcttgtagag ggtcattcta cgataggaat tccgcacaat caacgaaccg gtcacggcga
    16621 agaagtccta gcttgcgata gcccagtcgg ttcgccgcca ggtaggccga ataacccgtt
    16681 gacttcatgc gggagcgcgg atacgatctc acgcatagct aggggagcca tacggctgag
    16741 agtccgagca ctcggacgac cctcagaacc tgacctgggt aataccagcg tagggaagcg
    16801 gatcaaccga agacgctggt gtctgagccg cactccctac tcgcgaggtg cggcttttta
    16861 tttggaacag atctacatcc atcaacgatc cgccacaatt ttcagttgtg gagacaagaa
    16921 cgcatcgctc gtggctgagg gctgaatgct caacgctact atgaaaggat ctcctatgag
    16981 cgactctacc agtatagttg gcaacgggaa aaagacgccg tcctcgacct tgacgaccgc
    17041 tccctttccc gcctcccgca aggtctttgt gacaggcctg cgacccggcg tacgagtgcc
    17101 gatgcg
//
LOCUS       DATIFR010000216         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_530064, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000216 DATIFR010000000
VERSION     DATIFR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530064"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VLD60_12435"
     CDS             complement(<1..181)
                     /locus_tag="VLD60_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45816.1"
                     /translation="MIDERESLSPTKVGLGVLWPAFWTGLPFKLAIAVLFLAMGMMQF
                     ETRIGLAFVMVLASPV"
     gene            complement(212..1324)
                     /locus_tag="VLD60_12440"
     CDS             complement(212..1324)
                     /locus_tag="VLD60_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HSB45817.1"
                     /translation="MEHSSIPSTQPTAIEKVIAFIDERIGLKQMQAKMLNEPVPGGSR
                     WAYVFGSILLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHEVDYGWFLLSYHFWG
                     STAMVVCVFAHMSQVFLWGAYKKPREMIWLVGLALFGIVMGFGFTGYLLPWDQRAYWA
                     TTVGVEIMDKTPVIGDFMARFLKGGPIPGQMTLSRFFVIHVMILPAALMGLAGLHLFL
                     FRAAGPAGPFRGTPEEIKAKTDYFFPRQIWKDIVGMAVVFASICALAFWEPVVLLEEA
                     TPDPGEYHPEPEWYFLFLFQMLRLKIFAGEFGQFLGAVALPGAFMVLLAALPFIDKDP
                     ERNIFKRPIALIGWIVVMVTILLFTVSAIINREFLD"
     gene            complement(1325..2260)
                     /locus_tag="VLD60_12445"
     CDS             complement(1325..2260)
                     /locus_tag="VLD60_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSB45818.1"
                     /translation="MMQPKLKSKVRCTDREIGEVSKVVVDPLSHEISHIVVSMNGSGE
                     RQVAMGEVQTVTDGLVQLRGASGDILALPPFKRDDYVTTHEVEIAHLEENVHVTPGEV
                     LVPFPELEKSVKRRTFFMNFTHAIGFLIGLPIAFPVLRYLMKPMYAPFDNRWLKIGNV
                     GKIKQEDIGVQFKYKKMVKEAYLPEAEVEKNVWILKATPDVLEKVFQGKDMEFRDATG
                     KTVWTNKKEIPYVAFSGKCPHLGCGYKWRKHKILGQVFLCPCHLSIYDASGKVLDGPA
                     PRPLDALPIKVSASGEVEIIDMEFKAGTKAQIRII"
     gene            complement(2261..>2719)
                     /locus_tag="VLD60_12450"
     CDS             complement(2261..>2719)
                     /locus_tag="VLD60_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB45819.1"
                     /translation="ARAKALDEQAKALAERIKALGGDQATASQASGGAGAPPATTGAA
                     GGDILKIGEDQWQLQECYNCHKLKGEGGKKRGPELDNIGSYLTVQDIKEKILDPKSWM
                     AEGFEKEYEKGKMPDKYKDLMEENDVIALATWLNTFKNTSVNTPKPIKKK"
BASE COUNT          606 a          808 c          721 g          584 t
ORIGIN      
        1 tcacagggct tgcgagcacc attacaaaag ccagtccgat cctggtctcg aattgcatca
       61 tgcccatcgc caggaacaga acggcgatgg cgagcttgaa gggcaggccg gtccagaagg
      121 ccggccacag gaccccgaga ccgactttgg tgggggatag agattcgcgt tcgtcgatca
      181 tgttcgcatc atgcggcgga cggtgccgcg ctcaatccaa gaattcacgg ttaatgatgg
      241 cggatacggt aaacagcaag attgtgacca tgacgacaat ccacccgatc aaggcgatcg
      301 gtcgcttgaa gatgttccgt tccggatcct tatcgataaa cggcaacgcc gccagcaaga
      361 ccatgaaagc gccaggcaag gccaccgcac cgaggaactg tccaaactca ccggcaaaaa
      421 tcttcaggcg caacatctgg aagagaaaca agaaatacca ctcgggttcc ggatggtact
      481 cgcccggatc cggggtggcc tcttccagca gcaccaccgg ctcccaaaag gcaagggcgc
      541 agatactcgc gaagacgacg gccatgccga cgatgtcttt ccaaatttgg cgaggaaaga
      601 agtaatcggt cttggctttg atttcttccg gtgtacccct gaatggccca gccggcccgg
      661 ccgcacggaa caggaagaga tgcagcccgg ctaaacccat gagggccgcg ggaagaatca
      721 tgacgtggat gacaaagaat cggctcaagg tcatctgtcc agggatcgga ccgcccttga
      781 ggaaccgcgc catgaagtcg ccgatgaccg gcgtcttgtc catgatctcc acaccgactg
      841 ttgtcgccca ataggcacgc tgatcccacg gcaacagata gccggtaaag ccgaatccca
      901 tcacaatgcc gaagagtgcc agacccacga gccagatcat ctcgcgtggt ttcttgtagg
      961 ccccccagag gaagacttgc gacatgtggg cgaagacaca gacgaccatc gccgtcgagc
     1021 cccaaaagtg atagctcaac aggaaccagc cgtagtctac ctcgtgaatg atgtattggg
     1081 tgctggcgta agcatgatcc gcagtcggca cgtaatagaa cattaagagg atgccggtca
     1141 ccgcctgcat gatgaagatg aacagcagaa tggaaccgaa gacataggcc caacgggagc
     1201 cgccgggaac cggctcgttg agcatcttcg cctgcatctg cttcagaccg atccgctcgt
     1261 caatgaaggc gataactttt tcgatcgcgg tcggctgcgt gctggggatc gacgaatgtt
     1321 ccatttagat gatccggatc tgggctttgg ttcccgcctt gaattccatg tcgatgattt
     1381 cgacttcgcc ggacgccgag accttgatcg gtaacgcatc taacggccgg ggcgcaggac
     1441 catcgagcac cttccccgac gcatcataga tactcaggtg acaagggcat aggaatacct
     1501 gcccaagaat cttgtgcttc cgccacttgt agccgcaccc gagatgcggg cacttccctg
     1561 agaatgcgac gtagggaatc tccttcttgt tcgtccacac ggtcttcccg gtcgcgtcgc
     1621 ggaactccat atccttaccc tgaaaaacct tctccagcac gtcgggagtg gccttcagaa
     1681 tccagacgtt tttttcgact tcagcctcag gcagataggc ttccttcacc atcttcttgt
     1741 acttgaactg aacaccaatg tcttcctgct taatcttgcc gacgttgccg atcttgagcc
     1801 atctgttgtc gaacggggca tacatcggct tcatcagata gcgcagaacc gggaaggcaa
     1861 tcggcaaacc gatcaggaag ccgatcgcat gggtgaagtt cataaagaag gtacgacgct
     1921 tgacgctctt ctccagttca gggaagggca cgagcacttc gccgggcgtc acatggacat
     1981 tttcttccag atgtgcgatc tcgacttcat gagtggtgac gtagtcgtcg cgcttgaacg
     2041 gcgggagcgc cagaatgtcg ccagaagcgc cgcgcaactg taccaagccg tccgtcacgg
     2101 tttgcacctc tcccatcgca acctgccgct cgccgctgcc attcatggac acgacgatgt
     2161 ggctgatctc gtgcgagaga ggatcgacga cgaccttgct cacttcaccg atttcacggt
     2221 ccgtgcaccg gaccttggat ttcaacttcg gctgcatcat ttactttttc ttgatcggct
     2281 tcggtgtatt caccgaggta ttcttaaacg tgttcaacca ggtagccagg gcgatgacgt
     2341 cattttcttc catcagatcc ttgtacttat cgggcatctt gcccttctcg tactccttct
     2401 cgaatccttc ggccatccag ctcttgggat ccagaatctt ctccttgata tcctgaaccg
     2461 tcaggtaact gccgatgttg tccagctcag gcccgcgctt ctttccgcct tcacctttca
     2521 gcttgtggca gttgtagcac tcctgcaatt gccattgatc ttcaccgatc ttgagaatat
     2581 cgccacccgc cgcgccggtc gttgcaggcg gagcgccggc ccctccggat gcttgcgagg
     2641 ccgtcgcctg gtcaccgcct aatgccttga tccgttcagc caacgccttg gcctgctcgt
     2701 ccagcgcttt ggcacgcgc
//
LOCUS       DATIFR010000217         1522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2065315, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000217 DATIFR010000000
VERSION     DATIFR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065315"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1059
                     /locus_tag="VLD60_12455"
     CDS             313..1059
                     /locus_tag="VLD60_12455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45820.1"
                     /translation="MDIPRIFNITESAHRIHNPITPEKLATLGAALRLESGTRVLDLG
                     SGSGEMLCTWARDYGVIGTGIDMSQLFTEQAKLRAEELGVADQVKFIHGDATGYVSDD
                     KAGVAACLGATWIAGGVIGTIELLAQSLRTGGIILIGEPYWRQLPPTEDVAKGCLANS
                     ISDFLMLPELLASFGHLGYDVVEMVLADQDGWDRYEAAKWLTMRRWLEANPDDELAKD
                     VRAKLTSEPERYAAYTREYLGWGVFALMPR"
     gene            1252..>1522
                     /locus_tag="VLD60_12460"
     CDS             1252..>1522
                     /locus_tag="VLD60_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45821.1"
                     /translation="MAEQQPSFDAAKYKNAQREQWNKDGAAWRRWNPTLDRWYGDVTC
                     QMLDLARIQPGQRILDIAAGAGEPAVSAAERVGPSGYVLATDISEG"
BASE COUNT          326 a          426 c          450 g          320 t
ORIGIN      
        1 aatgatggtg acaaatcgtt ctgtgcaagc ttgttggtgc tgaacggatc acttgaatca
       61 aagcttatgg gttaataatt ataccgcgat gtccttcctg tttatgttaa tctggcctta
      121 acgcgggacc gtacaagttc catgcagaag atgtgaatga taaggacttg cagcggttct
      181 atgtaagaat tggcagttct cttgcaggct ggtaaagaac gaaaattcgt gtttcactag
      241 ccggaggcgg ctagataatc attggttagg cagtgaccga atctattcac acccgaaagg
      301 agattttctg cattggatat tccacggatc ttcaacatca ccgaaagtgc tcaccgcatc
      361 cacaacccga tcacgcccga aaagctcgcc acgctcggcg cggcgctgcg tctggaatcg
      421 gggacccggg tgctcgacct cggcagcggt tcgggggaga tgctgtgcac ttgggcacgc
      481 gattacggcg tcatcggcac cggcatcgac atgagccagt tgttcaccga gcaagcgaaa
      541 ctccgcgctg aagaactcgg cgtcgccgat caagtcaagt tcatccatgg cgatgctacc
      601 ggctacgtct cggacgacaa ggccggtgtg gcagcctgtc tcggtgctac atggatcgct
      661 gggggagtca tcggcactat cgagcttctg gcgcagagcc tccgcaccgg agggatcatc
      721 ctcatcggcg agccctactg gcggcagtta ccgccgacgg aagatgttgc caaggggtgt
      781 cttgccaact caatctccga ctttctcatg cttccagaac ttctcgcgtc tttcggccac
      841 cttggctacg acgtcgttga aatggttctg gctgaccaag acggctggga tagatacgag
      901 gcggccaaat ggctcaccat gcgccgatgg cttgaagcca atcccgacga cgagctcgcc
      961 aaagatgttc gagccaaact gacctcggag cccgagcgct acgccgctta cacgcgtgaa
     1021 tacctgggct ggggtgtgtt cgcgctgatg ccacggtgaa gcgcgatggc gagtagtttg
     1081 tgcactgaat tgccgccttg gcctaaccat cgcttcgagg ggaccgccga gaagctgcgc
     1141 ttctcggttc ccttcgccgt gcgggctccg gcggcccctc aagctgaacg ttagcagtct
     1201 gagtctcccg ccgcgaagcg gcattaaggt ctaaccgaga ggagaacttg cgtggccgaa
     1261 cagcaaccct catttgatgc agcgaagtac aagaacgcgc agcgagagca atggaacaag
     1321 gacggcgcag catggcgacg ctggaatccc actctggatc gttggtatgg cgacgttacc
     1381 tgccagatgc tcgacctggc ccgtattcag cctggtcagc gcatcctgga catcgccgcc
     1441 ggcgcgggtg aaccggctgt cagtgccgcc gaacgagttg gtcctagcgg ttacgtgctg
     1501 gcgaccgaca tctcggaagg ga
//
LOCUS       DATIFR010000218         9272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1106409, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000218 DATIFR010000000
VERSION     DATIFR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106409"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VLD60_12465"
     CDS             <1..285
                     /locus_tag="VLD60_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HSB45822.1"
                     /translation="DEPTSGLDLTATFQYLDLVRTYMRKGTTLLLVTHHIHEIPPEVK
                     RVVLLKKGKILHDGQKRTVLTETNLSLLFDCSVALAEANGWYQALPGASR"
     gene            complement(298..1329)
                     /locus_tag="VLD60_12470"
     CDS             complement(298..1329)
                     /locus_tag="VLD60_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB45823.1"
                     /translation="MRPYQPVPFQSYLTVLSILIVSATLGVYGVAEPLASQAQDSFTR
                     AQQATVGILEDTQDPRAPDKPGKISVRGTGVHLRDGYVVTARHAVEKHGVSGPIVMKQ
                     IRILTTDLHELPAQLIGESAYLDVVLYRIPEQHRALLSGPAPFAAGDVAAGTEVFTIG
                     YPLGWGPTMAFGRVGNANTFLQTVDTRLIQVDLSACSGNSGGGLFNMNGEVVGIMHAV
                     IQTDKEETHAHCSQMAFAVPATLAQRAVTAAMEGKPLAFSKLGVHMTSVKDGTKWRIA
                     VKDVTDPAKEAGLQKHDVLLTIDDTEILDAAQLKNYLIERTMPGQKVAVKVRRVDADL
                     TFTVTLAGG"
     gene            1467..1781
                     /locus_tag="VLD60_12475"
     CDS             1467..1781
                     /locus_tag="VLD60_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45824.1"
                     /translation="MVIRRTRATALWVAVMGVNAFWCVGMAASAEGNVQETPGVVSQI
                     GTALKNAATRIEQGVTSVVKQLEESETPKKIGNELKRSANALGEKAERAGKKLKESFK
                     SE"
     gene            complement(1799..2248)
                     /locus_tag="VLD60_12480"
     CDS             complement(1799..2248)
                     /locus_tag="VLD60_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HSB45825.1"
                     /translation="MIEEEQTCPPSPPSASAWEAILADPPWTAKSFLAAGAATIGGLA
                     SWIGNLSSPALAKFGGSYLGGFLLGWAFRHFLKMAAMMAAVVLASIAVLKATGWLDLD
                     WATIETQVSQGMRWLQGEAEGLKNLMAGLLPSAGAAGAGAFFGFRKK"
     gene            complement(2760..2939)
                     /locus_tag="VLD60_12485"
     CDS             complement(2760..2939)
                     /locus_tag="VLD60_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45826.1"
                     /translation="MTRIISMFALAAFLSVGFGTVMTFATEPAPAEQKEKKDMNGGKA
                     DEEKKKEDKQGVSGK"
     gene            3332..3922
                     /locus_tag="VLD60_12490"
     CDS             3332..3922
                     /locus_tag="VLD60_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSB45827.1"
                     /translation="MKTTPLARVIAFNKPYDVLPCFTDPEGRPTLADYIELPGVYAAG
                     RLDRDSEGLMILTSDGALVHRITDPKHKLPKVYLAQVERIPDEQALNQLRHGVVLSGK
                     RTRPAAVRLLDEEPLLPDRPVPIRFRKNVPTAWLEITLHEGMNRQIRRMTAAAGHPTL
                     RLVRVGIGPIALGDLQPGQWRELSLEEIARIGKTAQ"
     gene            complement(3916..4050)
                     /locus_tag="VLD60_12495"
     CDS             complement(3916..4050)
                     /locus_tag="VLD60_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45828.1"
                     /translation="MASRGLITEQPIDTARDAVYDRAGFEGFYFLSAPLSPGKITLGY
                     "
     gene            complement(4096..4920)
                     /locus_tag="VLD60_12500"
     CDS             complement(4096..4920)
                     /locus_tag="VLD60_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45829.1"
                     /translation="MGGQTFSDPIAQGYYRQGESEISTAQSAEDVLQKADALARHDSR
                     AHMMESACYYLAAAHFLETRDPAKSAHAYHQAGRQLQHLDQFIHAARAFSQSGRWAER
                     AAQAESPGSTQQHLQHAAVRAYSRANHCFAEAGELDESETAYLKERDARLAWAKMQGS
                     HPLALLVWKTTSKYGTSIPRWTGWMVVTIVVFSVLYEAFFRMQWLQPMSNTDPAGWIP
                     LWSGFYYAINVTSSLALVEYQPTHPVAQAVVMLNVIAGYLFLGIGIGIIGHLIKSR"
     gene            5142..6308
                     /locus_tag="VLD60_12505"
     CDS             5142..6308
                     /locus_tag="VLD60_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45830.1"
                     /translation="MFSCFPPVKGAFLDTCVGVACIVIALSGCTSGEVKKGTGPVCED
                     LEKRINLSERIETLNLLSSAFTARCDDLVATYGAQARSQFRHKTFSITRETANVFVPD
                     GTFIDYVMESYERGYLSMLLAASYLRMHNVVDAKVELRDLDHELFAPIYNFGEDPVNL
                     LLSAVLWEQLGDIREARVDWLRLRDFQVFAMQDDQVVRRFAGVRVDQIDRGDSAGAQW
                     RVYRIGTFPQLDWDLQFTNSTSGYFSITPKQPFVTSCASDTGVRMSTETWFDKLAMRH
                     SHAYHPLLNVQTWIRLPVGLTYSLIPVVAGAGVMVGGCMLDAAGNGKGALCQLSVVGG
                     IALMGTAPRVLEGALRPDLRHWEDVPAAVVVTTATEPDREPCMPKQGHQAYPLL"
     gene            complement(6483..7691)
                     /locus_tag="VLD60_12510"
     CDS             complement(6483..7691)
                     /locus_tag="VLD60_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSB45831.1"
                     /translation="MLAVSGCSVVLALKQPEKKNLTVLESGTPRSIVIKELGAPTKTE
                     EVQGTNIDVFQFSEGYSKELKMLRAGIHLTVDAFTFGIWEPFGIYLEQHIRPPDTSLE
                     VHYDANDRLTRFKVLSGENILLATSPADTPSAPVMPTLSPFVTPVIMPQTITSYPQRL
                     AVIQPQGPTASFVSSGLDLTLAYLRTFHPTMAIVERDRLEPVTQELVLQHTGKMHDDT
                     MARLGGWKGADTLLIVRIEQPSADRLQPITQGGGEVAHLVEIRLAEVETGLLLFRQTT
                     SARVQVPAPSGTQAWPDQLLEDARRETLRVAYTYAMTSLTAAFGDNPLGLVPDMSSRS
                     GGIRLLGLLHGGPGHNAGLQTGDRILEVDGVPYRSVTQRIPLPTMLLVERGNERKNVR
                     VEALGFGKTH"
     gene            complement(7792..8829)
                     /locus_tag="VLD60_12515"
     CDS             complement(7792..8829)
                     /locus_tag="VLD60_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB45832.1"
                     /translation="MTWNETPLFMRRRTILKAVGLGALTGMTGGCDAVGSVFGRMFAV
                     PPRDTTYFTPNSKFYVVNYADSAVSMSRDVNIEQWKLHIKGVVRTPLSLGWRDILNRD
                     SYDQVSTLMCIDTLPGGDSLGNATWRGISLKKLLIEVGADDETARDVIFRGIDGYDDS
                     IPFTRAMQDDVMLAFLMNGEKLPKEHGFPVRLIVPGLYGIKNVKWITEIEVYPGDYLG
                     YWQRKGWTDEGTIKIFSRIDSPGHYQALRGPEQKFRGIAFGGPNSISKVEISFDAGHT
                     WNGCEIETPMSAYSWVIWNYSWKPPKQGKFQTVVRATDTKGQLQIAEIVRPQPAGASG
                     LHTIIAEVEQI"
     gene            9137..>9272
                     /locus_tag="VLD60_12520"
     rRNA            9137..>9272
                     /locus_tag="VLD60_12520"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1999 a         2459 c         2762 g         2052 t
ORIGIN      
        1 gatgaaccga ccagcgggct cgatctgacc gcgacgttcc agtatctgga cctcgtccgc
       61 acctacatgc ggaaggggac cacgcttctc cttgtcacgc atcatattca cgagattccc
      121 ccggaagtga aacgggtcgt gttgttgaag aaggggaaga tccttcatga tgggcagaag
      181 cgcacggtgc tgacagaaac caatctgagc ctgctgtttg attgttccgt ggccctcgca
      241 gaagcaaacg gctggtatca ggctctcccc ggtgcttcga ggtagcggcg ccgcgaacta
      301 tcctcccgcc agcgtgaccg taaatgtcag gtctgcatcg acccgtcgga ctttgaccgc
      361 cactttttga ccgggcatcg tgcgttcaat cagatagttc ttcaactgtg cagcgtcgag
      421 gatttcggtg tcgtcgatgg tgagcagcac gtcatgtttc tgaagcccgg cttctttggc
      481 cgggtccgtc acgtctttca cggcaatgcg ccatttggtc ccatccttta ccgacgtcat
      541 atggaccccg agcttcgaga aggccagcgg tttgccctcc attgcggccg tcacggctcg
      601 ttgggctaag gtggcgggga ctgcaaatgc catttggctg caatgggcgt gagtttcttc
      661 cttatcggtt tgaatgaccg catgcatgat gccgacgact tccccgttca tgttgaagag
      721 gccaccaccg gagtttccgc tgcaggcgga gagatcaacc tgaatgagcc gtgtgtccac
      781 ggtttggaga aacgtattcg cattcccgac tcgtccaaat gccatcgtgg gaccccaacc
      841 gagcgggtaa ccgatggtaa agacctccgt gcctgctgcg acgtcgccgg cggcaaatgg
      901 ggctgggcct gagagaaggg cacggtgctg ttcgggaatt cgatacagga cgacatccaa
      961 atacgcgctt tcgccgatga gttgagcggg aagttcgtgc aagtccgtgg tcaggattcg
     1021 aatctgtttc atgacgatgg gcccggacac cccgtgtttt tcaacggcat gtctcgcggt
     1081 tactacgtag ccgtcgcgca gatgcacacc ggttcctcgc acggagattt tccccggttt
     1141 gtccggtgca cggggatcct gcgtgtcctc tagaattccg acagtggctt gttgggcacg
     1201 ggtaaaggaa tcctgagcct ggctcgccaa cggctcggct acaccgtata cacccagagt
     1261 agccgatacg atgagaattg ataaaacagt caggtacgac tgaaagggaa cgggctgata
     1321 ggggcgcatg agatgtcctt tcgagtgcgg gttcctattg gtccagtata aagctatgca
     1381 gccggtgcgg gaagaggggg tggggtgata ctgtctctcg atgcattgtg gtattgtgaa
     1441 tttatatgaa agaaggtaga ggcaaaatgg tcatacggcg gactcgggcg acagcactat
     1501 gggtagcggt gatgggtgtc aacgcgttct ggtgcgtcgg gatggccgcc tcggcagaag
     1561 gaaatgtaca agaaactccc ggcgtcgtga gccagatcgg aacggcgttg aaaaatgctg
     1621 cgacaagaat cgagcaggga gtaaccagcg tcgtgaaaca acttgaagag agtgagacgc
     1681 cgaagaagat tggcaacgaa ctcaagcggt cggccaatgc gcttggggaa aaggctgaac
     1741 gggcggggaa aaaactgaaa gagtctttca agtcagaata gagggtcctt ccggggtcct
     1801 acttctttcg aaatccgaaa aatgctcccg cccctgccgc tcctgcagag ggaagcaacc
     1861 cggccataag attcttcagg ccttcggctt caccctgaag ccatcgcatg ccctgtgaga
     1921 cctgcgtttc gatcgtggcc cagtcaaggt ccagccagcc tgtggccttt aacaccgcga
     1981 tcgaggcgag aaccacggcc gccatcatgg cggccatttt caaaaagtga cgaaaggccc
     2041 agccaagcag aaacccgccc aggtagctgc ccccgaattt cgccaaggca ggagagctaa
     2101 gattcccaat ccaggaggcg agtcccccaa tcgtggcagc tccggcggcg aggaacgatt
     2161 tcgcggtcca gggtggatcg gccagaatgg cttcccaggc ggatgcgctg ggcggtgagg
     2221 ggggacaggt ttgttcttct tcaatcatga acgactccga tgcgaatgac aggcggttcg
     2281 cgcagtgcgc cctgtgcaac tctcttggcg cgatcacgat acgtgttcct caatggggaa
     2341 gtccagaggg tgaaggggag aggacgcagg aggtcttcgg catgctgaag acctcttgac
     2401 atcgtggttt ggctacagat ccgtgaatca gtttgctggt tcaagctttc gaccaccttg
     2461 gacaggtctg tgggatagag gacgttctcg attcgccgga tgctggagcg gccatctttc
     2521 gtatcattgg aggcttgggt tttggtgtcg gtgtagcccc cctgcggacg ggtattgagg
     2581 gtccagacct tcctgatcga tcagagatcg tacctaggtg gtagctcgtg ccgtggtgag
     2641 ttcccaattc gtcttgtatc ggtctttgcg ctttgtgctt tggagtactt ggctgttgtg
     2701 ctgatgacac gggaccaaca ggaactgggg cgcaacgtgt gttgcgcccc aggatggaat
     2761 tactttccgc tcacgccctg cttgtcctct tttttcttct cttcgtcggc cttgccgccg
     2821 ttcatgtctt tcttctcttt ctgctcggcc ggagcaggct cggttgcaaa ggtcatgact
     2881 gtgccgaatc caacggagag aaatgcggct agcgcgaaca tggaaataat acgagtcatg
     2941 ataaaacctc cttggttgag aaacacataa caatgcgtgc ctcccctgac acaccattcg
     3001 gaacatttcc gctgagggga cagcgtacga gggagagagc aaatgcggtg ccgggcggtt
     3061 tacaccaccg gatcactcac tgccgagtgg gaaatcgagg aattgtgaaa ggttccgaag
     3121 atgaagggtt ctgggaagtc acctcagatg aaattacgta tatctcgaag agaaggtgtt
     3181 ggctgtaggc gattacctgc aaccatacag tttgcggtcg aatcgaattc ttagattcct
     3241 ctcatcatct gtaggcgaca cattcacgta aaagcatgct tatttcggag gtgtttcttc
     3301 tgtgctgtcg atgtgggaaa ctcatacatc gatgaagacc acgcctctcg ctcgtgtgat
     3361 tgccttcaat aaaccgtatg atgtgctgcc gtgctttacc gatccggagg ggcgaccgac
     3421 gttggccgac tatatcgaac tgcccggtgt ctacgcggcg ggccgactcg atcgtgacag
     3481 cgaggggctg atgattctca cctcagatgg cgcgctcgtt catcggatta ccgatcccaa
     3541 gcacaagctg ccgaaggtat atctggccca ggttgagcgt ataccggacg aacaggcgct
     3601 gaatcagcta cgccatggcg ttgtgctaag cgggaagcga accagaccgg ccgcggttcg
     3661 cttgctggat gaagagccgt tgcttccgga ccgtcctgtg ccgatccgct ttcgcaaaaa
     3721 cgtaccgacg gcctggctcg aaatcacact gcatgaaggg atgaatcgcc agattcgtcg
     3781 catgaccgct gcggcgggac atccgacgtt gcgactcgtg cgcgtgggaa tcggtccgat
     3841 cgctctcggt gaccttcagc cgggacaatg gcgggaatta tccctggagg agattgcgag
     3901 gattggtaag actgctcaat aacccaaggt gatcttgccg ggcgataatg gagcggagag
     3961 aaaatagaaa ccttcgaagc cggcacggtc gtaaacagca tctcgggcgg tgtcgatcgg
     4021 ctgttcggtg ataagcccgc gcgaggccac cgaggaacac ctccacgccg aagagtgatc
     4081 gaaacgtgaa gtggcctagc gactttttat gaggtggcca atgataccga tgccgatgcc
     4141 gaggaagaga taaccggcga tcacattcag catgaccacg gcctgcgcaa cgggatgggt
     4201 tggttggtat tcgaccagcg cgagggacga cgtgacattg atcgcatagt agaaaccgga
     4261 ccagagggga atccaaccgg ctggatcagt attgctcatc ggctgcagcc actgcatgcg
     4321 gaagaaagct tcatacagca cgctaaacac gacgatcgtc acgaccatcc aacccgtcca
     4381 tcgggggatg ctggtgccgt atttgctcgt tgttttccag accagaaggg cgaggggatg
     4441 gcttccttgc atcttggccc acgcgagccg cgcgtcgcgc tccttcagat aggcggtttc
     4501 cgattcgtcc aactctccag cttccgcgaa acagtgattg gcacgtgagt aggcgcggac
     4561 cgcagcatgt tgaagatgct gttgcgtgga acctggagat tcggcttgcg cggctcgttc
     4621 agcccatctg ccggattggg agaaggcacg tgccgcatga atgaattgat ctaagtgctg
     4681 tagttgccgt ccggcctgat ggtatgcgtg ggcagatttg gccggatcac gggtttcgag
     4741 aaaatgtgcg gcggccaggt agtagcaggc ggactccatc atatgggcac gcgaatcgtg
     4801 tcgggcaagc gcgtcggcct tctggaggac atcctcggca gactgtgccg tggagatttc
     4861 gctttcgccc tgccggtaat aaccttgagc gatcggatct gaaaatgtct gcccgcccat
     4921 acatccctcc aggctgggcc atgttaccct aacgcgaggt cggcaaggaa gggctcttgc
     4981 ctggcgcgaa cgacctgcgc gtgctttgga aagtctcctg cgacttcccc ggtcaactca
     5041 gcatgcacct gacagccgcg tcactaatct agaaagtctg tcggcactca ggtagactcc
     5101 cgcatcaatc ttcttgcgcc ttcagaaatt cgaggagtgg tatgttcagt tgtttcccgc
     5161 cagtgaaggg agcattcctc gatacctgtg tcggcgtggc ctgcatcgtt attgcgctaa
     5221 gcggctgtac ctccggagaa gtgaagaagg gaaccggtcc agtctgcgag gatcttgaga
     5281 agaggattaa tctctcggag cgaatcgaga cgctgaatct cttgtcatcc gcattcacgg
     5341 cccgttgcga cgaccttgtg gcgacgtacg gagcgcaagc ccgatcacag ttcaggcaca
     5401 aaacgttctc gatcacgaga gagactgcga acgtgtttgt cccagacggc accttcatcg
     5461 actacgtgat ggaaagctat gagcggggct acctcagcat gctgctggca gccagctatc
     5521 tcagaatgca caacgtcgtg gatgccaagg tagagctgag agacttggat catgagctgt
     5581 tcgcgccgat ctacaacttt ggagaagatc cggtcaacct tctgctctcc gccgtcttgt
     5641 gggaacagct cggagacatt cgtgaagcgc gcgtggactg gctacgtctc agagactttc
     5701 aagtctttgc aatgcaagat gatcaagtcg ttcgccggtt tgccggggtt cgagtggacc
     5761 agatcgacag gggagatagc gccggggccc agtggcgcgt gtaccggatt ggcacgttcc
     5821 cccagcttga ctgggacctg caatttacca attccaccag cggctatttt tccatcacgc
     5881 ccaagcagcc gttcgtcaca tcgtgcgcat cggacaccgg agtacggatg tcgacagaaa
     5941 cctggttcga caagctcgcg atgcgccata gccatgccta tcacccactc ctcaatgtgc
     6001 agacgtggat ccgcctccct gtggggctga cttacagcct aattcccgtt gtggcgggag
     6061 ccggcgtgat ggtcggaggt tgtatgcttg atgcagccgg taatggcaaa ggagcactct
     6121 gtcagctgtc cgtcgtgggg ggaatagcgc tgatgggaac ggcccctcgc gtgctggaag
     6181 gcgctctgag accggacttg cgacattggg aggacgtgcc cgcagctgtc gtcgtcacga
     6241 cggctactga gcctgaccgg gaaccttgta tgccgaagca gggacatcag gcatacccac
     6301 tgctgtaggc ctgctcaact agcatgcgat tgggaagagc aatccgaccc gtaatgggat
     6361 gaaggagagc ggatggtagg gtgcgatctc acgaaaatag gcttatggca atggatacta
     6421 tcttcgtttc acatttgtga tgatcgtgtg gagctcactt gcttccgatt ggttcagccg
     6481 aatcagtgtg tctttccaaa tcccaaagcc tccacccgca catttttccg ttcgttccct
     6541 cgctcgacca gaagcatagt gggaagcgga atgcgttgtg tcacgctcct gtagggaacg
     6601 ccatcgactt cgaggatacg atcacccgtc tgtaaacctg cattgtgccc cggccctcca
     6661 tggaggagtc ccaacaggcg gatacctccg ctgcgggagg acatatcggg gaccaatccc
     6721 aaggggttgt cgccgaacgc cgctgtcagc gaggtcatgg cgtacgtata ggcgacccgc
     6781 aacgtttcgc gccgagcatc ttctagtagt tgatccggcc aggcttgagt gccgctgggt
     6841 gccggaactt ggactcgtgc cgatgtcgtc tgccggaaga gcaacagccc cgtttccact
     6901 tccgcaaggc gtatttcgac taaatgcgcc acttcacctc cgccctgtgt gatgggttga
     6961 agccgatcgg ccgacggttg ctcaattcgc acaatgagca atgtgtccgc tcctttccac
     7021 ccgccaagcc gggccatggt gtcatcatgc attttccctg tgtgctgtaa gaccagctcc
     7081 tgggtcacgg gttctaatct gtcgcgttcc acgatagcca tggtgggatg gaatgtgcgg
     7141 aggtaggcga gggtgagatc caggccggac gagacaaagg acgctgtggg gccctgaggc
     7201 tggatgacgg cgagtctttg gggatacgaa gtgatcgttt gcggcatgat cacgggagtg
     7261 acgaacggcg agagcgtcgg catgaccggg gctgatggtg tatcagcagg ggacgttgcc
     7321 aacaggatgt tttcacccga gaggactttg aaccgtgtga gtcggtcatt ggcgtcataa
     7381 tggacctcca gagaggtgtc ggggggacga atgtgttgtt ctaggtagat gccaaatggc
     7441 tcccatatgc cgaacgtgaa cgcatccaca gtgagatgga ttcctgcgcg gagcatcttg
     7501 agttccttgg agtagccttc tgaaaattgg aacacatcga tgttggttcc ctgcacctcc
     7561 tccgttttgg tcggcgcgcc caattccttg atgacgatcg agcggggggt tccggactcc
     7621 aggacagtca ggtttttctt ttccggctgc ttgagcgcca ggacgacgga acagccagag
     7681 acggcgagca aaagacaaac gagggagcac caacacaaaa gagagactgt tcgaatgatc
     7741 ggaagttctc cgtaatctct tgcagattgg actgccgtcg cagtaggcaa attagatctg
     7801 ttccacttcc gcaataatgg tgtgcaaccc gctcgcgcca gccggttggg gccggacaat
     7861 ctcagcgatc tgcagctgtc ccttcgtatc cgtggcgcgt acgacggttt ggaattttcc
     7921 ctgcttcggc ggcttccaac tgtagttcca gatgacccag gaataagccg acatcggtgt
     7981 ttcaatctcg cacccgttcc aggtgtgacc agcgtcgaaa ctgatttcca ctttgctgat
     8041 gctattgggt ccgccaaagg ctatgccgcg gaatttctgc tcgggcccgc ggagtgcttg
     8101 ataatggccc ggcgaatcga tgcgtgaaaa aatcttaatc gtaccctcat cggtccagcc
     8161 tttgcgctgc cagtagccga gataatcacc gggatagact tctatctccg tgatccattt
     8221 cacgttcttg atgccgtaaa ggcccggcac gatcaggcga acgggaaagc catgttcttt
     8281 cggcagcttt tcgccattca tcagaaacgc gagcatcaca tcgtcttgca tggcccgcgt
     8341 gaatggaatg ctgtcgtcat aaccatcgat gccgcgaaag atcacatcgc gcgccgtctc
     8401 gtcatcggca ccgacttcga tgagcaattt cttcagtgag atgccgcgcc aggtcgcatt
     8461 gcccaggctg tcgccgcctg ggagcgtgtc gatgcacatc aacgtagaca cttgatcgta
     8521 cgaatcgcga ttcaaaatat cgcgccagcc aagggacagc ggcgttctca cgacgccttt
     8581 gatgtggagt ttccactgtt cgatgttgac atcgcgcgac atggacacgg cgctgtcggc
     8641 gtaattcacg acatagaact tggaattcgg cgtgaaatag gtagtgtcgc gcggagggac
     8701 ggcgaacatc cgaccgaaga cggaacctac cgcatcgcag ccaccggtca tgccggtaag
     8761 tgcaccgagg ccgacggctt tgagaatcgt gcggcgccgc atgaacagcg gggtttcatt
     8821 ccaagtcatg atgccaaagt atacacggaa ttgatgcttg actctcgcaa aattccctgg
     8881 tacatttcgc ctcgttgatt cgtgcttgtg ttcctgcctg atgaccgcgc ctgacggcga
     8941 gtcacggtgc ggttccaaag ggaaactacc cgaaggaaat ttcctcgagc gggtcccttg
     9001 acacgctgcg tcgcacgttg tatagtgcgc ggctcgcgtg atgagcgcga tcgttctttg
     9061 acaactgaat agaggacgtt gagcaaggtg taaggtgtat tgaagtggtg gcccttggtc
     9121 caaattctaa gaacacctat atgagagttt gatcctggct cagaacgaac gctggcggcg
     9181 cgcctaatac atgcaagtcg aacgagaatg tgtagcaata cacatgtaca gtggcgaacg
     9241 ggtgaggaat acatgggtaa cctaccctcg ag
//
LOCUS       DATIFR010000219         1030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2641358, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000219 DATIFR010000000
VERSION     DATIFR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641358"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..900
                     /locus_tag="VLD60_12525"
     CDS             163..900
                     /locus_tag="VLD60_12525"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSB45833.1"
                     /translation="MDRLDPEDMGPGHPRSESRKVVQTFRHFVHEGVGWRTLTASNQY
                     VSGSTLRRWMGRWFATGILRAIHRALVLALRSDPTAAAEAVGLVVDSCSVRAKHGGDL
                     VGPNPTDRGKPGTKYHVVTLTDGTPLGAVASAANVNDTMMFPHLLKLAQAVCASIGKV
                     FADAGYDSAFNRDLCRKQGGDPVIRRRGDDHGSGLGKVRHIVENALSWVLSNKRLDRR
                     HDRSTLVVQSLLNFACVYVVAHRLAEL"
BASE COUNT          212 a          325 c          288 g          205 t
ORIGIN      
        1 ctaaggcact gttttcacaa tttgccgctt cggaccagct ccctggagag aaaccgcctc
       61 gacttgacca atgccaatca agcgccatgt caggacccac cataatcatc cgttcggagg
      121 taaacgataa gaccgtcggc catcgatctc ctcacgcccc tgattgatcg acttgatccc
      181 gaagacatgg gtcctggtca cccacggtcg gagagcagga aggtcgtgca gaccttccgt
      241 cactttgtcc acgaaggggt tggatggaga acgctcaccg cgtccaacca atacgtcagc
      301 ggttccacct tgcggcgctg gatgggacgg tggtttgcga cggggatctt gcgcgcgatc
      361 caccgtgcgc tcgttctggc gttgcgatcc gatcccacgg ccgccgccga agcggtcgga
      421 ctcgtggtcg acagttgcag tgtgcgagcc aagcacggcg gcgatcttgt cggtccaaat
      481 cccacagatc gtggcaagcc tggcacaaaa tatcatgtcg tgacgctgac agatggcacg
      541 cctcttgggg ccgtcgcctc cgcagccaac gtcaatgata cgatgatgtt tccacatctc
      601 ctgaagcttg cccaggccgt ctgtgcgtcg attggaaaag tcttcgctga tgccggctat
      661 gacagcgcct tcaaccgaga tctctgtcga aaacagggag gggaccccgt catccggcgg
      721 cgcggagacg atcacggctc cggcctcggc aaagtcaggc acatcgtcga gaatgcactc
      781 tcctgggtgc tgtccaacaa gagactcgac cgaaggcacg atcgatcgac cctggtcgta
      841 caatcgttgc tcaattttgc ctgcgtctac gtggtcgcgc atcgcttggc cgaattgtga
      901 aaacagcgcc taacgcagac acgggtccct ccgcttccct acagacgcac ttgccgggtt
      961 accgtggccg gtgtctgcca cgccgcccga gccagatcag cagactcaac ggcttgagac
     1021 caccttccat
//
LOCUS       DATIFR010000220         4204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_534237, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000220 DATIFR010000000
VERSION     DATIFR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534237"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="trpS"
                     /locus_tag="VLD60_12530"
     CDS             <1..732
                     /gene="trpS"
                     /locus_tag="VLD60_12530"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSB45834.1"
                     /translation="IPEHAILHLLLSMMIPVSWLERNPTYKEKQEELKEKDLSTYGFL
                     GYPVLQAADILLYKPDFVPVGKDQLPHLELTREVARRFNDLYKKSVFPEPKEHLTKFP
                     KVLGTDGRKMSKSYGNTINLSDTEPVVRQKLKTMVTDPARVRRTDPGNPDLCPVYEFH
                     KIYSPTGVQEQINKECRTAAIGCIDCKKLVADRLVEQMTPIWDARANLVQHPSRLDEI
                     VQEGSRKAGEVAKATLHDVTEAMKI"
     gene            complement(767..1387)
                     /locus_tag="VLD60_12535"
     CDS             complement(767..1387)
                     /locus_tag="VLD60_12535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45835.1"
                     /translation="MPIGRRSHDTTSKAVLQTVAAYERSAHEFLDRWGRRRYRLPALL
                     RQWLSRLPKRARLLDLGCGGGQDAHYLWRQGYRVIGLDRTPALLSFARRRSRTLPLIL
                     ADMGRLPLRYRSVDGIWAAASLIHLPKAIVRVVLADLLDLTQPGGILAATFTHGTKSR
                     VLSRGWISGRYVARWRKDELERALCSAGWRVVDIRVVLDRSAGGDG"
     gene            1410..1706
                     /locus_tag="VLD60_12540"
     CDS             1410..1706
                     /locus_tag="VLD60_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45836.1"
                     /translation="MKRLRIAACLVGLIGGNAWAVDPPCDKYPPAKQARCADIWTALN
                     KEDGPTIAQFGLDQLKRREAGKITTEQHLAQNMEFIRHSTEKRLERLKDRMARE"
     gene            complement(1711..2217)
                     /locus_tag="VLD60_12545"
     CDS             complement(1711..2217)
                     /locus_tag="VLD60_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSB45837.1"
                     /translation="MIKIGPFILSIVLVGLALFPSAQAGEPQATPSESVTISNVEVRL
                     SDHGPVVLLQAEGKSIPIFVDLTVAMSIQGALKGEKLSRPLSHDLMHSILETFGGKVT
                     QTVITLKGGTYFGALTVAFREDVKVFDSRSSDSIALAIHFKAPIFVGRDLLESAGRVL
                     EKSKAEDL"
     gene            complement(2236..2874)
                     /locus_tag="VLD60_12550"
     CDS             complement(2236..2874)
                     /locus_tag="VLD60_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB45838.1"
                     /translation="MRTLIRKTFSVPPLGCNCSIIGDPVTKQAIVVDPGGAHERILHD
                     VNQLGLTVTMILHTHAHFDHFLASGELKKATGAILCLHEQDRELWGMLDIQCRMFGVP
                     YAPVPPPDYWLKDEEQLFIGQVKAVALHTPGHTPGSMSFHVPEGKLVLAGDTLFRGSI
                     GRTDLWGGDFDAIEQSIRERLYTLEDHTAVVTGHGPETEIGLEKESNQFFRA"
     gene            3011..3763
                     /locus_tag="VLD60_12555"
     CDS             3011..3763
                     /locus_tag="VLD60_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45839.1"
                     /translation="MMKNSSVRHQQNLASIRVSFLSIVALLAFVGCKPDDSPFKAENE
                     TLRKQLARQESLVTSLQDGNKVMQQQIDLLNQEIRDAKKAAESAKGEAKAAAEQLESQ
                     LMQARKLTADIKRTAIEQAAQSIRVESKGAQTEDIPRPLSTVAKTVEEALARNGYQVK
                     VSIMADQKAVFVTERKVSNPASLEVAGFRNQYVVSLQAQPTNVTRLSVKADFEKLAQG
                     GRILSVSAEETAEIERRLIGEVSKALASPHKT"
     gene            3871..>4204
                     /locus_tag="VLD60_12560"
     CDS             3871..>4204
                     /locus_tag="VLD60_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB45840.1"
                     /translation="MTAIAALAQSSPIVTVSAGQFILGSKRIDDDPYGLWTQFDDTEL
                     PQQLVWLDQYEMDRDEVSLGEFLAFLRQRKQHPPDELQKLIWHLITVHSVTDQTLARW
                     PALYVTWKE"
BASE COUNT         1007 a         1243 c         1154 g          800 t
ORIGIN      
        1 attccggaac atgcgatctt gcatttgctc ctttcgatga tgatcccggt gtcctggctg
       61 gagcggaatc cgacctacaa ggaaaaacag gaagagctca aagaaaagga cctcagcaca
      121 tacgggtttc tcggctatcc ggttcttcaa gctgcggaca ttttgctcta caagcccgat
      181 ttcgtccccg tcggaaaaga ccagctccct catctcgaac tcacacgcga agtggcaaga
      241 cggtttaacg acctctacaa gaaatcggtg tttcccgagc cgaaagagca tctgaccaag
      301 tttccgaaag tcctcggtac cgatggccgg aagatgagca agagttacgg aaatacgatt
      361 aatctctccg atactgaacc ggtcgtccgt cagaagctca agacgatggt gaccgatccg
      421 gcccgggtca gacggacaga tccaggcaac ccggaccttt gtcctgtgta tgaatttcac
      481 aagatctact cgccgaccgg cgtgcaggag cagatcaaca aggagtgccg caccgcggcc
      541 atcggttgca tcgattgcaa gaagctggta gcggatcgac ttgtcgagca gatgactccg
      601 atctgggacg cgcgcgccaa cctcgtgcaa cacccgtcac ggctggacga aatcgttcaa
      661 gaaggcagcc gcaaagccgg cgaagtggcg aaggcgacgc tgcatgatgt cacagaggcc
      721 atgaaaatct agcccggtga aactgagggc cgctgggcca ttacattcat ccatcgcccc
      781 ctgcgctcct gtccaggaca acccttatat cgacaaccct ccagccggct gagcaaagtg
      841 ccctctccag ttcatccttc ctccatcgcg cgacatagcg tccggaaatc catccccgac
      901 tgaggacccg acttttcgtc ccgtgcgtaa aggttgcagc gaggattccg ccaggctgag
      961 tgagatcgag caagtcggcc agtaccaccc gaacgatcgc tttcggcaga tggattaacg
     1021 atgctgcggc ccagatgcca tcgacgcttc tataacgcag cggaagccga cccatgtcgg
     1081 caaggatcag cggcaaagtc ctcgatcggc gtcgcgcgaa ggagagcaag gcaggtgttc
     1141 gatcgagccc gatcaccctg tatccttgtc gccagagata atgagcgtct tggccgcctc
     1201 cacaaccaag gtcaagcagc cgggcccgct tcgggagacg ggacaaccac tggcggagga
     1261 gcgcaggcag ccgatagcgg cgtcgccccc accgatccag aaattcatgc gcagaccgtt
     1321 cgtaggctgc gacggtctgc agaacggctt tggatgtggt atcgtgcgat cggcgaccga
     1381 ttggcatgag gtgaaggaat caggcgggca tgaaacggtt acgcatcgcg gcctgtctcg
     1441 taggactcat cggaggaaac gcatgggcgg tggatccccc ttgcgacaag tatccacccg
     1501 cgaagcaggc tcgttgcgcg gatatctgga ccgccttgaa taaggaagac ggtccgacca
     1561 ttgcccagtt cggcctggac caactgaaac ggcgcgaagc cggcaagatc acgaccgaac
     1621 agcatctcgc ccagaacatg gaattcatca ggcactcgac cgagaaacgg ctcgagcgct
     1681 taaaggatcg gatggcgaga gaataggccc tcacaaatct tctgctttcg atttctccaa
     1741 cactcgtccg gctgattcca gcagatcccg tccgacgaag atcggcgctt tgaagtgaat
     1801 cgccagtgca atggagtcgg acgaccggct atcgaacact ttcacgtctt ctcgaaacgc
     1861 cacagtcaac gcgccgaaat aggtgccgcc tttcagcgta atgaccgtct gcgtcacctt
     1921 cccgccgaac gtctccaaaa tcgagtgcat gaggtcgtgc gataacgggc gcgacagttt
     1981 ttcccctttc aaggcgcctt gaatcgacat ggcgaccgtc agatcgacaa agatcggaat
     2041 gctcttccct tcggcttgaa gcagcacaac cggcccgtga tcggagaggc gaacttccac
     2101 attgctaatc gtcaccgact ctgagggagt tgcctgaggc tcaccggctt gggcggacgg
     2161 gaacagggcg agaccaacaa gaacgatcga gagtatgaat ggacctatct tgatcatggt
     2221 cacctcccca gcgcatcagg ctcggaaaaa ttgattcgat tccttttcca gtccaatttc
     2281 ggtttccggt ccatgccctg tcaccactgc cgtatggtcc tccagcgtgt agagccgttc
     2341 gcggatcgac tgctcgatgg cgtcaaaatc tccaccccaa agatccgtcc gacctatgct
     2401 tccccggaac agcgtatcac ccgccaacac gagctttcct tccggaacat ggaaactcat
     2461 ggaacccggt gtatgaccgg gtgtatgcag cgccacggct ttcacctggc caatgaacaa
     2521 ctgctcctca tctttcagcc aataatccgg tggaggcacg ggggcatagg gcactccgaa
     2581 catccggcat tggatgtcga gcatgcccca caattctcga tcctgttcgt gtaagcataa
     2641 aatcgcgccc gttgcctttt tcaactcccc ggaagccaga aaatggtcga agtgggcgtg
     2701 cgtgtggaga atcatggtga ccgtcagccc cagttggttg acatcgtgga ggatgcgctc
     2761 atgggccccg cccggatcga ccacgatggc ctgcttcgtc actgggtcgc cgatgatcga
     2821 gcaattgcac cctaacggag gcacggagaa ggtctttcgg atcagtgtcc tcatggtttt
     2881 cacgctctca atatgaatta acggtatcgt ccggcacgaa aggatagcct gctgccgccg
     2941 gaagggtcaa acaacggtcg tgtgagaatc ctcgtggaca acagcctgcg aagcatggat
     3001 aatcgccgcc atgatgaaaa actcttcggt gcgtcaccaa caaaacctag cgtcgattcg
     3061 cgtcagtttc ctcagcatcg tggccttgct tgccttcgtc ggctgcaaac ccgacgattc
     3121 gcctttcaaa gcggaaaacg aaacactccg caaacaactg gccagacagg agtcgctcgt
     3181 cacgtcgctg caagacggga ataaggtcat gcagcagcag atcgacctcc tgaatcagga
     3241 aatccgcgac gccaaaaaag cggcagaaag cgccaaaggc gaagcgaagg ctgccgccga
     3301 gcaacttgaa agtcaattga tgcaagcgag aaagttgacg gcggacatca aaagaaccgc
     3361 catcgagcag gccgctcaga gcatccgcgt cgagagcaag ggggcgcaga cggaagatat
     3421 tcctcgtccc ctgagtacgg tcgcaaaaac cgtcgaagaa gcccttgccc gaaatggtta
     3481 tcaggtcaag gttagcatca tggccgacca gaaagccgtg ttcgtcacag aacgaaaggt
     3541 ttccaatccg gcatccctcg aagtcgccgg attccggaac caatatgtgg tcagcttgca
     3601 agcacagcca accaacgtca cccgcctgag cgtgaaagcg gatttcgaga aattggctca
     3661 gggcggacgc attctcagcg tcagcgcgga agaaacggca gagatcgagc ggcgcctcat
     3721 cggcgaggtc agcaaggcac tcgcgtcgcc ccacaaaacg taatgctccg ctcaaagtcc
     3781 ttggtcgcac cttttgttct tgctctcgca ctgttgggtg cgacagcggc cgtagctggt
     3841 acaaagccct cgacggagtt ggcccgccac ttgacggcca tcgccgccct tgcccaatca
     3901 agccccatcg tgaccgtttc ggcgggacaa ttcatccttg gcagcaaacg gatcgacgac
     3961 gatccttacg gcctttggac acagttcgac gatacggaac taccgcagca actggtctgg
     4021 ctcgaccagt acgagatgga tcgggacgaa gtcagtctcg gcgagttcct cgctttccta
     4081 cggcagcgga agcagcatcc ccctgacgaa ttgcagaaac tcatctggca cctcattacg
     4141 gtccattccg tgacggatca gaccctggcg cgttggccgg cgctctatgt gacatggaag
     4201 gagg
//
LOCUS       DATIFR010000221        11986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1875948, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000221 DATIFR010000000
VERSION     DATIFR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875948"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..141
                     /locus_tag="VLD60_12565"
     tRNA            67..141
                     /locus_tag="VLD60_12565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Arg,seq:ccg)
     gene            179..255
                     /locus_tag="VLD60_12570"
     tRNA            179..255
                     /locus_tag="VLD60_12570"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:213..215,aa:Lys,seq:ctt)
     gene            complement(485..1006)
                     /locus_tag="VLD60_12575"
     CDS             complement(485..1006)
                     /locus_tag="VLD60_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HSB45841.1"
                     /translation="MDIHRCLYRHSGTGGSGTRTAVRKSSRRVIAVNLGGAVIPAILS
                     IYLIVKNKLYLSSLVAVGIVTIVVHMMSYPVPGVGIAEPFFIPPMIAAIVAIVVSRQH
                     AAQLAYIAGSLGTLIGADLLNLSLIQGLGAPVASIGGAGKFDGIFLTGLIAVVLASIA
                     TRKQRTSRTGIAC"
     gene            1317..1718
                     /locus_tag="VLD60_12580"
     CDS             1317..1718
                     /locus_tag="VLD60_12580"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HSB45842.1"
                     /translation="MKKVFTVLVTSCAALLIILNGQIFADDMDNGNYLLKICNSAMKT
                     MQEPKELDVEWSELINSAECRSYINGYANAIEFENKNNNLKVKYICFPDKAGLQLWRD
                     VYKYLQDHPQELRHPKSLLVYKAFKEYYPCK"
     gene            complement(1837..2364)
                     /locus_tag="VLD60_12585"
     CDS             complement(1837..2364)
                     /locus_tag="VLD60_12585"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HSB45843.1"
                     /translation="MTKTPNSGDSRGPQPGGLRVVAFFEGAKGGLVLITGFGILAFIH
                     RGLHSAAEEVVRHFHLNPAHHYPRIFLDAAARFNDTQLWLLALSAFLYAVVRFIEAFG
                     LWHRKLWAAWFGVLSGGIYIPVELLEVAHGLSWAKVTVLSVNIAVVIYLGYSLAIPGQ
                     SPRNPSIPSAPRPPG"
     gene            2737..3330
                     /locus_tag="VLD60_12590"
     CDS             2737..3330
                     /locus_tag="VLD60_12590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB45844.1"
                     /translation="MWHERIVSFLPNRWKFRWRYWRRQTPWDTQVTPPEVMEFIARTP
                     PGKALDLGCGTGTNAITLARHGWRVTGVDFIPKAIFAARAKAARSGVAIDFLVASVND
                     LSALSGPFDYALDIGCLQSLKAEDRMRYAAHLSRLLRPQAWYMLYAWLPRPSVGGVAG
                     ISTEAVESLLRTDFSKVRTAIGEEKGYPSAWYWFQRR"
     gene            complement(3600..4214)
                     /locus_tag="VLD60_12595"
     CDS             complement(3600..4214)
                     /locus_tag="VLD60_12595"
                     /inference="COORDINATES: protein motif:HMM:NF020663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB45845.1"
                     /translation="MKKTAVFAILLAVTVSAYAKEGGTVRSITLPVVETPLADGEGKV
                     VVTSNCSSCHSLDYIVMQPKLSPDAWTGTVNKMRKVFGAEISDADAGVIVSYLAMNYG
                     TGRKSAAVDAPGKDPAPKAAMKISGEASFKKYCNACHAGGGNIIRPTKTLHRKDREAN
                     GVKTAVDIARLLRNPGPGMPKFDEISVPEAEAKAIAEYVIDTFK"
     gene            complement(4231..5427)
                     /locus_tag="VLD60_12600"
     CDS             complement(4231..5427)
                     /locus_tag="VLD60_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB45846.1"
                     /translation="MADHGLTRRGFIRTAGVVTALAATGKFPIDLFAAEKPRLIRYPE
                     KTDLILLTSRPPQLETPQRFFKELLTPNDALFVRWHLGNIPTEVDLATWRLAVGGNTG
                     RTLELSMDDLRTRFEKVEYTAVIQCSGNGRSFFDPRVPGGQWGNGAMGNITWSGARLK
                     DILAMAGVKPDSSEVAFNGLDGPPAPKVPDFVKTLPMDLALQEDTIVAYEMNGKPLLM
                     LNGFPARLVVPGWFGTYWVKALSEITVLSKEFDGFWMKPAYRIPDNPCACVAPGGKQA
                     KTIPINRMNTRSLIISPESGVTFKLHQPVEIMGIAFAGGYGIKDVIVSTDSGKSWGTA
                     QLGKDLGKYAWIQWRYPWKPGKAGTYRLMAKATNSIGESQPSGRLWNPSGYMWNTVET
                     TEVVVR"
     gene            6212..6559
                     /locus_tag="VLD60_12605"
     CDS             6212..6559
                     /locus_tag="VLD60_12605"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB45847.1"
                     /translation="MEKTDIFELHADLCKTLSNPKRLMILALLAKKEMSVGEIAEVIA
                     VPLSNVSQHLALLKAQSIVQTRKEGQTVYYSLADRRIIEACTLIRSVLLDKMKERGQV
                     ARKIDPRYIVAAL"
     gene            6696..6950
                     /locus_tag="VLD60_12610"
     CDS             6696..6950
                     /locus_tag="VLD60_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45848.1"
                     /translation="MRKFAIGTGLVVFSAMLSVQAYAAGSSHTDYVKKYEGTASCTTA
                     LCHEGAAKEVAESLHYQQRAVPQFLEGWEAGKLAGMMESY"
     gene            7152..8045
                     /locus_tag="VLD60_12615"
     CDS             7152..8045
                     /locus_tag="VLD60_12615"
                     /inference="COORDINATES: protein motif:HMM:NF023211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSB45849.1"
                     /translation="MVKEGDKFRIAPDPSIDVLKAAQSVGKPTNEMCLRCHIGAGGGP
                     NHKHGVTPTKDSDVHVAKGMQCLDCHTARKHKVAGGSDIKAQELLDVKVACENCHATP
                     HKGGKAKILDRHTARIACQTCHIPTIARDPKMPTIVARDWTKPVLNEKTGLYGPTNKM
                     AGNFRPEYAWWNRIMKIPPEPAGSIADPKSKIYPWKRTEYTVIGDAATGKPIYIKSGV
                     YAVTGDPGAAAKKGAEDGKQNYSGNWKGVQETMVFSMNHQVASKGEALKCDSCHSPQG
                     VLDFKKLGYGEERIKKLSGGY"
     gene            8132..8647
                     /locus_tag="VLD60_12620"
     CDS             8132..8647
                     /locus_tag="VLD60_12620"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HSB45850.1"
                     /translation="MLHKNRRIPVIGILAAMFLVPAFLAIAAGPAGKAVSPIFGIAVP
                     EGYRNWALIAVSHRTDDKDELRAILGNPVAVEALRKGTLPLPDGAMLAKLAWKREAMP
                     EFQGAFKPGASPRLEFMVKDSKKYASTGGWGFARFIDGKPADEATHKTCFPCHEANVK
                     GHDFVFTRFAP"
     gene            9304..10659
                     /locus_tag="VLD60_12625"
     CDS             9304..10659
                     /locus_tag="VLD60_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSB45851.1"
                     /translation="MAHFPKESDDLARGAIRLRFPADAEAAFRRYHSRNSLVVIRYAI
                     LLTIILYGLFAVLDVHAAPLSKQTIWFIRFVLVIPALVAVLIFSFHKIFLRILQPMMV
                     LVVLIDASGILAMIDLESHGELGYQTYYAGLMLVIMAAHSMYRLRFVYATFSTILVLV
                     GYEYIAIVHQKLIVSEVGFAVFVSNNFFFVSSIILGMAASYFLEVFLRRVFAQRIRLA
                     QEQEKSERLLRNILPRETAAALKEQEGVIADQYDSASILFADVADFTSMSARMSPHEV
                     VELLNGLFSHFDDLAERHDLEKIKTIGDCYMLAAGVPRTCHNHAHVLTHVGLAMLRYV
                     KGRRFGNVGQISLRIGINSGPVVAGVIGRRKFRYDLWGDTVNVASRMECHGTSGRVQI
                     TTSTYELIKDQFVCEPRGSILVKGKGAMPIWHVIAEKASFSTGVQSPETHLLDPLVNT
                     G"
     gene            complement(10912..11388)
                     /locus_tag="VLD60_12630"
     CDS             complement(10912..11388)
                     /locus_tag="VLD60_12630"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSB45852.1"
                     /translation="MKKGAMALMALGLVLVFAGTVSAAGATKDECVAKTKEAAAMVNA
                     KGLDASIVEVNKKDGKFVWKDSYVFLVDFDGKMLAHPMSPTLIGKNVLDMKDKAEDPA
                     KGKFLFKEFTEMAKAKGEGWVEYMWANPGDPKPRKKITYIYRVPGKNIYAGAGIWE"
BASE COUNT         2488 a         3407 c         3487 g         2604 t
ORIGIN      
        1 agagcaccag actgtggctc tggctgtcgc gggttcaatc cccgtcactc accccatcat
       61 cttcaggcgc ctgtagctca ggggatagag caccggcctc cggagccggg tgcggaggtt
      121 cgattcctcc caggcgcgcc atgatctttc cggtgcggta ttcgttcttt cccagcgtgg
      181 gccattagct caacccggta gagcagctga ctcttaatca ggaggttgta ggttcgaatc
      241 ctacatggcc caccagtaat ttcagggggt tagaccactt cggtctgacc ccttttttcg
      301 tcccggcgcg tgggcggcgc gttttggagc cctgtgccca gccgattgac cgctgacctg
      361 tcccctccgg gaagggcgtg actctaagcg atcaatttcc agcatcttcg ctgccccgtt
      421 ttgcgaggga aaacatgggg aaaagtctgt atggactgca aaccccgtgc aaacgactcc
      481 ctatctaaca cgcgatcccc gtgcgggaag tacgttgttt acgggtcgca atgctcgcca
      541 gaacgacggc gatgaggccc gtcaggaaga tcccgtcgaa tttcccggct cccccgatcg
      601 aggccacggg cgcgccaagc ccctggatca aggagagatt aaggaggtcg gcgccgatga
      661 gggttccgag gcttcccgca atataagcga gttgggccgc atgttgccgg gagacgacga
      721 tggccacgat cgcagcaatc atgggcggga taaaaaaggg ctccgcgatc ccgacgccgg
      781 ggacgggata ggacatcata tgaaccacga tcgtcacgat gcctaccgcc acgaggcttg
      841 agagataaag tttgttcttc acgatgagat agatggaaag aatcgcgggg atcaccgcgc
      901 cgcccaggtt tacggcaatc accctacgtg acgatttcct gactgccgtc ctggtgccgc
      961 tgccccctgt tccggaatgg cgatataggc atcgatgaat atcaattcat ggattatttt
     1021 acttaaacac gatcgatatt gatacgaaga gactgtgcca aaaccccgcc cgtaatgctt
     1081 gggggctttc cgttcacgca atcccccggt gcctccccat catgttctct gacgaatagt
     1141 aggttccccg cctccacaag attccctctc catgaacgcg caggaaatcg aagggaaccc
     1201 gcacagacct caaatctcac ctgactgcac cgcgttaggg cggtcatttg ttccgtgtta
     1261 acatgaggca tggttagtcc aggaatgcac aattcaggga gaaggggaga tatccgatga
     1321 agaaagtatt cactgtactg gtgacatcct gtgccgctct attgataata cttaatggtc
     1381 aaatatttgc agatgatatg gataatggca attatttatt aaaaatatgt aattcggcga
     1441 tgaagacgat gcaagaacca aaagaattag atgttgaatg gtctgaactt attaacagtg
     1501 cggaatgcag atcctatatt aatggttatg ctaatgcaat tgaattcgaa aataaaaata
     1561 ataatttgaa agttaaatac atatgcttcc cggataaagc cggtttacaa ttatggcgtg
     1621 acgtttataa atatttgcag gatcacccac aagaattacg ccatccaaaa tcgttattag
     1681 tctataaggc tttcaaagag tattaccctt gcaagtgaag catgaaatgg tgaattttat
     1741 tcaagtcctc tattaacatg taccatcctt gtacttatgt agtataggtg gatatcgcca
     1801 tcctgctcca acagactggc cggatccgct taacgtttat ccgggcggac gcggggcgct
     1861 gggaatggag ggatttcgag gtgactgtcc ggggatcgcg agcgaataac ccaaataaat
     1921 gacaaccgca atattgaccg atagtaccgt cactttggcc caggagaggc cgtgggccac
     1981 ctctaagagt tccaccggga tgtagatgcc tccactcagg actccgaacc atgccgccca
     2041 aagtttccga tgccataagc caaatgcttc gatgaaccgg acaacggcat aaagaaacgc
     2101 tgaaagagcc aggagccata gttgggtgtc gttgaatcga gcggcggcgt cgagaaagat
     2161 acgggggtaa tggtgggcag gattcaagtg gaagtgtctt acaacttctt cggccgcgct
     2221 gtggaggccg cgatggatga aggcgagaat cccgaagccc gttatgagga ccaaccctcc
     2281 tttagcgcct tcaaagaatg cgaccacccg tagaccgccg ggctgcggac ccctgctgtc
     2341 gccgctattt ggagttttgg tcattcattt ccggtgaggg aaagaaaatt ctaatagagt
     2401 cgccgaaata atgtggttgt gccaccctgc gggtttcaat ggcggcaaac tgttccagtt
     2461 cacggttgct ccgttgcgat tccgattcga ggattgtcag tttcttctcc gccgcatgtc
     2521 gtttttcata tagaccggtc agaaaatgca ttgagtggcc aggggaagat tccggcgaga
     2581 acggaaatga ggaacaggtc tttgcccgat ttcaaataca atccctcaga ttcctggtcg
     2641 gaacattctt tccaacagaa ttattctact ctcgtgatgg ggaggttctg aatgtccctt
     2701 cttcgtttgg cttggtgatg gagggggttt ccgggaatgt ggcatgaacg gatcgtctcc
     2761 ttcctgccca accgctggaa attccggtgg cgttattggc gccggcagac cccttgggac
     2821 actcaggtca cgccgcccga ggtgatggaa ttcatcgccc ggaccccccc cggaaaagcg
     2881 ctggatctgg gatgcggcac gggaacgaat gccatcaccc tcgcccggca cggatggagg
     2941 gtaacgggcg tcgatttcat acccaaagcc attttcgcag ctcgcgccaa agcggcccgg
     3001 agcggagttg cgatcgattt tcttgttgca agcgtcaacg acctttccgc cctgtccggt
     3061 cctttcgact atgctctgga catcggctgc ctgcaatccc tgaaagcgga agatcggatg
     3121 cgctatgccg cgcacctgtc ccgattgctg cggccgcagg cctggtacat gctttacgcc
     3181 tggttgccga ggccatcggt cggcggggtt gccggcatct ccaccgaagc ggtcgaatcc
     3241 ttgctgcgga cggatttctc caaagtccgg acggcgatcg gggaagagaa aggctacccc
     3301 tccgcgtggt actggttcca gcggcgttga ggggaccgat ttccaagcgc tcctttcggg
     3361 gagagccgaa tcgagcaact ggctgggttg tactgcaacc cccgtgcaaa caacgggtgt
     3421 tttcgggggg aaagaggggt ttcgccggga ggtgatcgac gagggaaatc ccttgtgcgg
     3481 ttggggagat ttacgagggt gggagttcac atgcctccgc accccccatg ctgtacctgc
     3541 gacttacgga gccggaggag gggcgaacgg tcgcccaccg tctccggccc cttgcgtgat
     3601 cacttgaacg tatcgatcac atactcggcg atcgccttgg cttccgcctc gggaaccgat
     3661 atttcatcga atttcggcat ccccggaccg ggattgcgga gcagccgggc gatatcgaca
     3721 gcggttttga cgccgttcgc ctcgcgatct ttccgatgca gggttttcgt gggccgaatg
     3781 atatttccac cgcccgcgtg acatgcgttg caatatttct tgaaggacgc ctctccggag
     3841 atcttcatcg ccgccttggg ggccggatcc ttgcccggtg cgtccacggc tgcgctcttc
     3901 cttcccgtcc cgtagttcat tgcaaggtat gacacgatga ctcccgcgtc tgcatcggag
     3961 atttccgctc cgaatacctt gcgcatcttg ttcaccgtcc cggtccacgc gtccggggag
     4021 agcttcggct gcatcacgat gtaatccagg ctgtggcagg aactgcaatt ggacgtcacg
     4081 acgactttac cctcgccgtc ggcgaggggc gtctcgacga cgggtaaggt gatggagcga
     4141 accgttcccc cctcttttgc ataggcggat acggtaacgg caagcagaat cgcgaagaca
     4201 gcggtctttt tcatggtccc cccttgtgtc ctacctgacg accacttcgg tcgtctcgac
     4261 ggtgttccac atgtatccgg acgggttcca taatctcccg gacggctgcg actcgccgat
     4321 gctgttcgtg gccttcgcca tcaggcgata cgtacccgcc tttccgggct tccagggata
     4381 acgccactgg atccaggcgt atttccccag gtcctttccg agctgcgccg tgccccagga
     4441 ttttccgctg tcggtggaca cgatgacgtc cttgatcccg tacccgcccg cgaaggcgat
     4501 ccccatgatc tccaccggct gatgcagctt gaaggtgacc ccactctcgg gcgatatgat
     4561 gagagacctc gtgttcattc ggttgatagg gatggtcttt gcctgcttgc cgccgggggc
     4621 cacgcacgcg caagggttgt ccggaatgcg ataggcgggc ttcatccaga agccgtcgaa
     4681 ttccttcgag agcacggtga tctctgaaag ggccttcacc cagtatgtgc cgaaccagcc
     4741 ggggacaacg agcctcgccg gaaagccgtt cagcatcaac aacggctttc cattcatctc
     4801 gtaggcgacg atcgtgtctt cctgcagggc gaggtccatg ggcagcgtct tcacgaagtc
     4861 cggaaccttc ggggcgggcg gcccgtccag tccattgaag gccacttcgg aggaatccgg
     4921 ctttactccc gccatcgcca ggatgtcctt gagccgggcg cccgaccagg tgatgttccc
     4981 catcgcgccg tttccccact gtccgcctgg cactctcggg tcgaagaagc ttctcccgtt
     5041 cccggagcat tggatgactg cggtgtattc gaccttttcg aaacgtgtcc gaaggtcgtc
     5101 catggaaagc tccagtgtcc tgccggtgtt tccgccgacg gcaaggcgcc aggtggcgag
     5161 gtcgacttcg gtggggatgt tgccaagatg ccaccgtacg aacagggcgt cgtttggcgt
     5221 tagcagctcc ttgaagaagc gttgcggggt ttcgagctgg ggcggccggg acgtcaggag
     5281 gataaggtcg gtcttctcgg ggtagcggat taatcgtggc ttttccgcgg cgaacaggtc
     5341 aatgggaaat ttcccggtgg ctgcgagagc ggtgaccacc cccgcggtac ggatgaaccc
     5401 tcttcgcgtc agcccatggt cggccatcgt ctcctccttg gctggtatgc ctgtgctggt
     5461 tgccctccga tgcccgccgt taactattct cccgtttctc cttcatggaa tggggctttg
     5521 catccatcga atctgcattg tcgcttcggt catcgatctc gcacagggtg aataccccac
     5581 tgcgccctgg tgaagcgata ttgacggctg ggtaacgact tgcgccatga atccactaca
     5641 gcgccttccc cctgccagta tattttctcc acgatcggcg tcaactccga attccgcaca
     5701 tacgtcctgc aagacctcgc cgcgcatggt atggaaccgt cggggttttg gcctttctag
     5761 ttcagaccgt ggacgggtaa cggatcgggg gaggactcgg ggatgaccgg cgcctgctgg
     5821 aagtggccga acacgtgcat cctccggccc caccgccatt ggagcagggc ggttacggtg
     5881 actgcacagt gggtaacacc gaatggaacg caaatcccgt gcaaataacc gacttttccg
     5941 gggaggagaa agatgagggg gtggtgggga aacccgtggg ggttcaaatt ccgcctacga
     6001 cgccggtggc atgttgacaa ccgactttcc cggcgcgcca gcggcgcgtc tcgcggctct
     6061 gtttaaccca tttcaacccg gcttgaccat ttcggattcc cggagaattg tctcgcatcg
     6121 cgaaaggaaa aacgttgaca ggtgatgatt cgttgctatc gtacttattg gatatcgcat
     6181 atatgcacta atggatattg atggaggaac gatggaaaag accgatatat tcgagttgca
     6241 cgccgatctg tgcaagaccc tctcgaaccc caagcggctc atgatcctcg ccttgctggc
     6301 caagaaggag atgagcgtgg gggaaatcgc ggaggtgatc gcggttcccc tctcgaacgt
     6361 gagccagcac ctggcgctgc tgaaggcgca aagcatcgtg cagacccgaa aggaaggaca
     6421 gaccgtctac tacagcctcg ccgaccgtcg gatcatcgag gcgtgcacgc tgatccggtc
     6481 cgtccttctg gacaagatga aggagcgtgg ccaggtcgcg aggaagatcg acccccggta
     6541 tatagtggcg gccctctgag cggcttatgg aatccaatcc gagaggaagt gaaagacggt
     6601 gtccaacgag aaggaggtgg gcggggctgc cgtagggggc agcaaaaaca tgattgcagc
     6661 ggtggattga acatcttggg gataggaggg agcgcatgag aaagttcgct atcggaacag
     6721 gcctggtggt cttttcagcg atgctgtcgg tccaagcgta tgcagcggga tcttcccaca
     6781 ccgattacgt caagaagtac gagggcaccg ccagctgcac caccgccctt tgccatgaag
     6841 gggcggcgaa agaggttgcg gagagcctgc attatcagca gcgagcggtc ccgcagtttc
     6901 tggagggctg ggaggcaggg aaacttgcag gaatgatgga gtcgtattga gcgcccccca
     6961 acacggtggt ggggcataac tggattggga tcctccaacc caaggatgcc tcgaaaccgg
     7021 cgcagccagg tggttgcgta cagtgtcacg caggactcgg cgcaaagccc aaccccgtcg
     7081 ggaggctgac ggaggcggat tataacaaca tcgattgcct tctttgccac gccccgggat
     7141 acaagcggac ggtggtaaag gagggggaca agttccggat cgcaccggac ccgtccattg
     7201 atgtcctgaa agcggcgcaa tcggtcggga aaccgacgaa tgagatgtgc ctccggtgcc
     7261 acatcggcgc tggcggcggg ccgaaccaca agcacggcgt caccccgacg aaagacagcg
     7321 acgtccacgt tgcgaagggg atgcagtgcc tcgattgtca tactgcgagg aagcacaagg
     7381 tcgccggcgg gtccgacatc aaggcccagg aactcctcga tgtcaaggtt gcctgtgaaa
     7441 actgccatgc cactccgcac aagggaggga aggcgaagat cctggaccgg cacacggccc
     7501 ggatcgcctg ccagacatgc cacattccga cgatcgcccg ggaccccaag atgcccacga
     7561 tcgttgcgcg cgactggacc aaacccgtgc tgaacgagaa gaccgggctt tacggcccga
     7621 ccaacaagat ggccgggaac ttccgtcccg aatacgcatg gtggaaccgc atcatgaaga
     7681 tccccccgga acccgcaggt tccatcgccg acccgaaatc gaaaatctat ccctggaaac
     7741 ggacggagta cacggtgatc ggggacgcgg cgaccggcaa accgatctac atcaagtcgg
     7801 gagtctacgc tgtcaccggc gatcccgggg cggcagcgaa gaagggcgcg gaggatggga
     7861 agcagaacta cagcggcaac tggaaagggg tccaggagac gatggtgttc tccatgaacc
     7921 accaggtcgc ctccaagggc gaggccctga agtgcgactc ctgccattcc ccgcagggcg
     7981 tgctcgactt caagaaattg gggtacggcg aggagaggat caagaaactg agcggcggct
     8041 attaaagaaa ggatcggtgc tggcggggtg gcctccgggc gtcccgccag cagtttatca
     8101 acacctcatc catttcctaa gggggtgccc catgctccat aagaatcgac ggattccagt
     8161 tattggcatt ctggcggcga tgttcctcgt ccctgcgttc cttgcgattg cagcgggacc
     8221 cgcgggaaaa gcggtctccc cgatcttcgg gatcgcggtc cccgaggggt atcgcaactg
     8281 ggcgctaatc gcagtatccc atcgcacgga cgacaaagac gagctgaggg cgatcctcgg
     8341 gaacccggtc gctgtggagg ctctgaggaa aggtacgctt ccactcccag atggcgccat
     8401 gcttgcgaaa ctcgcgtgga agcgcgaagc gatgcccgaa ttccagggcg cgttcaagcc
     8461 cggcgcctcg cctcggctcg agttcatggt caaggattcg aagaagtacg catcgaccgg
     8521 cggctggggc ttcgcccgct tcatcgacgg caagccggca gatgaagcta ctcacaagac
     8581 gtgcttcccg tgtcacgaag ccaacgtgaa gggacacgac ttcgtcttca cgaggtttgc
     8641 accttgatcg gcattcatac cgggcgatgt tgcgaggggg tggttgggaa acccgtgggg
     8701 gttcgagtcc ccccttcggt accgattgac gaccaatctt cccggcgcgt gagcggcgcg
     8761 tttggcggct ctgtttgacg ttgtaggttc gaatcctaca tggcccacca gcaaatccaa
     8821 ggggttacgg tgaaagccgt agcccctttt tctttctgtt cgcgtcccgt ccgcattccg
     8881 tcctgacgga tccttcagac gtccggctga cgacccagac ctacgcgcac gtgcaggcgg
     8941 aggcgcaccg gagggccgga ggagtgctga accggcttgg gttgaagggt tacggggtac
     9001 ggggaccgct cacctctgac aatggcttac tgaaggaatg gctaaaatgc ggcgctcaac
     9061 gtggacggcc cttgatacgg gtagccctga ttgacaggca gccgggggga atataaaatt
     9121 gttttacaac atcgatttgt aatctctcga gacaatcgca gagccgtccc ggcaagcagg
     9181 gaggcgtgat gcgggttctc gactttttcc atttcttgaa ttctccccct tgtgcgataa
     9241 ccccctcgtt cagagtgcga gccttggcag cagggctcaa tctttaacgg gaggatcagc
     9301 gggttggcgc attttcccaa agaatcggac gacctggctc gtggagccat aagacttcgc
     9361 tttcctgccg atgcggaagc ggcctttcgg cgttatcatt cccgtaattc ccttgtcgtc
     9421 atccgctatg ccatcctcct taccatcatc ctctatggcc tgttcgctgt tctggacgtc
     9481 catgccgccc cattgagcaa acagacgata tggtttatcc gctttgtcct cgtaattccc
     9541 gctctcgttg ctgtcctgat cttcagcttc cacaagatat ttctccgcat ccttcaaccg
     9601 atgatggttc tggtggtcct catcgatgcc tccggcatcc ttgccatgat cgacctcgag
     9661 tcccacgggg aacttggcta ccagacctat tacgccgggc tgatgctcgt catcatggcg
     9721 gcacacagca tgtatcgatt gaggttcgtc tacgccacct tttcgacgat tctcgtgctg
     9781 gtcgggtacg aatacatcgc catcgtccat cagaagctca tagtctccga agttgggttt
     9841 gctgtttttg tcagtaacaa tttcttcttc gtgtcgtcga taatcctcgg catggcggca
     9901 agctatttcc tggaagtgtt cttacgacgc gttttcgccc agcgcatccg tctggcccag
     9961 gaacaggaga aatccgagcg gctcctccgc aacatcctcc cgcgagagac ggcggctgcc
    10021 ctcaaggagc aggaaggcgt aatcgccgac caatacgaca gcgcaagcat cctgttcgcc
    10081 gatgtggcgg acttcacgtc catgtcggcc cgcatgagtc cccatgaggt cgttgaactg
    10141 ctgaacgggc tcttttccca cttcgacgat cttgcggagc gacacgacct cgagaagatc
    10201 aagaccatcg gagactgcta catgttggcc gcgggggtgc cgcggacgtg tcacaatcat
    10261 gcccatgttc tcacccacgt tggtctggct atgctgagat atgtaaaggg acgccggttc
    10321 ggcaacgttg ggcagatatc actgcgcatc ggcataaatt ccggaccggt cgtggccgga
    10381 gttatcgggc ggagaaaatt ccgctacgat ctctggggcg acacggtgaa cgtggccagc
    10441 cggatggagt gccacgggac gagcggcagg gtgcagatca caaccagcac gtatgagctg
    10501 attaaagacc agtttgtttg cgagccgagg ggatcgatct tggtgaaggg gaagggggcg
    10561 atgcccatct ggcacgttat cgccgaaaag gcatcctttt cgacaggggt gcaatccccc
    10621 gaaacgcact tgcttgatcc cctcgtcaat acagggtgag atgtccctct gggtacaaaa
    10681 taacgggagg aaactggcgc caaaccggat ggaaaactgt acgcaccgca aaccccatgc
    10741 aataacttcc attttgtggg tgaaaaaggg gtttcccgca gatgctgttg gcgggggaac
    10801 cccccgtgct gttagagagg tttcagggag gcaggccaag aggcccaagg gggtgggaaa
    10861 tggggccggc ccggatgcgc cggcccccga tggcttcctg ttcttgcgag actactccca
    10921 gatcccggca ccggcgtaga tgttcttccc cggcacacgg taaatatagg tgatcttctt
    10981 ccggggcttc gggtcgccgg ggttggccca catgtactct acccagcctt cccccttggc
    11041 ctttgccatt tccgtgaact ccttgaagag gaatttcccc ttcgcagggt cctccgcctt
    11101 gtccttcatg tcgaggacgt tcttcccgat cagcgtcggg ctcatcgggt gagccagcat
    11161 tttcccgtcg aagtccacga ggaagacata ggaatccttc catacgaact tcccgtcctt
    11221 cttgttgacc tcaacaatgg aggcgtcgag ccccttcgcg ttgaccatcg ccgccgcttc
    11281 cttggtcttg gcgacgcact catccttcgt cgcgcccgct gcggatacgg ttcctgcgaa
    11341 aaccaggacg agccccaagg ccatgagagc catcgcaccc ttcttcattg tttcctctcc
    11401 tttcgctcca gtaggtttgt gggttgcttc ccgccgatcc atcaccccct tggttcgcgc
    11461 cggtattacc ctcgtgtgcc acctcctttt tggtcgggtc ggaaggctgt cacagacacc
    11521 catacggcat ttcgtctgcc gacaatgcaa tggatttatc gtccgcctca aggatttctc
    11581 cagtcatgtt atcagcgccc ggagccgttt gaaccgccca gcgcacgatt tttcttacac
    11641 cggaggaggt ttgttcctgc cgtagccgga tctggccgcc atgtcctttg catggtccct
    11701 cgctatggcc gtcgcccggc ctctttccgg gttggtcctt tcgcatcggg acggttcctt
    11761 tgagtggcaa gggggatgcg gtatgggtgg tgttaagcgg tcgaaaagaa aaagcccggc
    11821 acggttgccg ggcttcctcc ctaaggcgcg gtaaacttcg gtgtggcgtt ccggtcgttt
    11881 ggttacctga cttccgactg caaccccact tcttttcaaa gacgagcaga gccaatccca
    11941 gccaagcgaa tcctttcaaa tccacgccaa ccgacgcgcc ctatgt
//
LOCUS       DATIFR010000222         5699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2836671, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000222 DATIFR010000000
VERSION     DATIFR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836671"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VLD60_12635"
     CDS             <1..768
                     /locus_tag="VLD60_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSB45853.1"
                     /translation="GDLRLESAAAKIIGSEWYWEAVNDLFQVRGGRGYMTVEAQRKSG
                     EPAIPVMRMLRDARINLVWEGTSEILRVWMAREALAPYIEQGMALLQGSLSDKIAAPL
                     YFARMSVRSCLPVMPPRRTSAIFGKEYTRWVRFIESSSRTLTRSTLLATLRHRQSLHN
                     KQLLLQDLVDDSLSLFPMAATLWFASQPEMRTKPGIRELVDYFCQEMADRLNPSSSPT
                     KRILGHTKDRTVYRLSKAIMSGEYAWLEEGILPLLEK"
     gene            complement(1211..2539)
                     /locus_tag="VLD60_12640"
     CDS             complement(1211..2539)
                     /locus_tag="VLD60_12640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB45854.1"
                     /translation="MIRSLRGKSVIVVGAGLAGLTAAVELQRQGATVTVLEGQGRVGG
                     RVLTIRNGFVERQYAEAGADFIDEEHEEICRLVRSLNLKLVPVLRAGFSFALSCDGKV
                     RRPVSGDGIWKRLTHHLAPLIHAYRLSEQRWDGAVAKKLGSVSVADWMEQVSLPDELR
                     HMLVGLRGFFLADPVNLSLLSLVDQVAAGSPGKGGMYRIAGGNDRLPEALASILGDRL
                     LIRHEVVAVSQSGTNVRVRIRAGNNQSWLRSDYLILALPATKVRTLDIRPALPREQAA
                     AISTLAYGPVTKTLLQFDHRFWKKRNQPLAYGTNLPIGAVWDANEQQKGKSGILCLMA
                     GGTASEATQKLLSTADAAGLVRTLDWLGKTRKKLVAMRSVTWGQDPWVEGGYAVFGPR
                     YDPTLRSWLARPCGRIFFAGEQTSLRWQGYMNGAVESGLRAAAEIQALTQ"
     gene            2822..3196
                     /locus_tag="VLD60_12645"
     CDS             2822..3196
                     /locus_tag="VLD60_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45855.1"
                     /translation="MTQLVDSLPAIAKRQPSAGAADGRQIITTHRTPNTLENLSPTDM
                     TPRLQTKLLKHHTILVTHLLLRLVTVQGASGRLLVRTGAADNIGGGKNLDPAFWMLGQ
                     SSVNRSHVLDKPWTISLAAEAL"
     gene            complement(3404..3985)
                     /locus_tag="VLD60_12650"
     CDS             complement(3404..3985)
                     /locus_tag="VLD60_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSB45856.1"
                     /translation="MKQDHAVPECAVFVGVSLDGFIARLNGDLDWLMGEGGGDSAEYG
                     YNEFIADIDAIVMGRRTFEKVLTFDKWYYGTKRVVVLSHQPLDLSVARSRGGVVELTA
                     GDPAEIVSMLTARGASRLYVDGGITIQQFLRAGLIHRLIISRLPVLIGEGIPLFGSLL
                     HDVRLSHIATRAYPGGMVQSEYHLVRTASGGSL"
     gene            complement(4069..4515)
                     /locus_tag="VLD60_12655"
     CDS             complement(4069..4515)
                     /locus_tag="VLD60_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSB45857.1"
                     /translation="MGINTIRLHRVFRATPERIYRAFLDADAMTKWLPPNGFTGKVHH
                     MDAKVGGTFKMSFTNFTTGKSHSFGGQYLELVPHERIRYTDNFDDPNLPGEIRVTVTL
                     KKVSCGTEVNIVQEGVPDVIPAEACYLGWQESLILLGKLVEAEIPN"
     gene            complement(4558..5322)
                     /locus_tag="VLD60_12660"
     CDS             complement(4558..5322)
                     /locus_tag="VLD60_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HSB45858.1"
                     /translation="MKKHKTGTREEWLAARLVLLKSEKELTRQSDKVVGQRQALPWVR
                     IDKQYRFETDEGSASLADLFRGRSQLLVYHFMFGPDWTAGCPSCSAIADGFNGFTVHL
                     ANHDVMLWAVSRAPLAKLQAYKRRMGWTFPWASSVGSDFNFDFNVSFTVEQQQGGMIE
                     YNYERGGHAMDATPAPLPVAQLGATCGTDASTYSRDRPGMSAFALEDGVVYHTYSTYS
                     RGVDAIWSMYPWLDRAPKGRNETGVWWRRHDEYEKR"
     gene            complement(5463..>5699)
                     /locus_tag="VLD60_12665"
     CDS             complement(5463..>5699)
                     /locus_tag="VLD60_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB45859.1"
                     /translation="LEPIQTAMSVRVRNGKLSVTDGPFAETKEQLGGFFLISARDLNE
                     AIQFASKFPSVRFGTMEVRPVRAFPGSSRAAASL"
BASE COUNT         1236 a         1742 c         1529 g         1192 t
ORIGIN      
        1 ggggatcttc ggttggagtc ggcggcagcc aaaattatcg gaagcgagtg gtactgggag
       61 gcggtcaacg acctctttca agtacgcggg ggacgcggat acatgaccgt tgaggcacag
      121 cgtaagagcg gcgaacctgc cattccggtc atgcgcatgc ttcgcgacgc ccgcatcaat
      181 ctggtgtggg aggggacgag tgagattctg cgcgtgtgga tggcgcgcga ggcccttgct
      241 ccctacatcg aacagggcat ggccttgctc caagggtcac tttcggacaa gatcgccgcg
      301 ccactttatt tcgcgcgcat gtcggtccgc tcctgtctgc ctgtcatgcc tccccggcgc
      361 acttctgcga tattcggcaa agaatacaca cggtgggttc gttttatcga gtcatcctcg
      421 cggaccctga cacgatccac cctcctcgcg acgcttcgcc atcggcaaag cctgcacaat
      481 aagcaactgc tcttgcagga tttggtggat gacagcctct cgcttttccc catggctgcg
      541 acgctctggt ttgcctccca accggagatg cgcacgaagc ccggcatccg cgagctggtc
      601 gattatttct gtcaagagat ggcagatcgt ctgaaccctt cttcctctcc gacgaaaaga
      661 atcctcgggc acacaaagga ccgcaccgtc taccgcctgt cgaaggcaat catgagcggc
      721 gagtatgcct ggcttgagga agggatccta cccttgcttg agaaataaca gaccggagca
      781 tagaagcctt ctccgccgtc tccatcgcca tcaaccggga actagggctt gatcccaaga
      841 gagtcaatgt caacggtggg ggcagtttgc cctgggccat ccgatcggca ccaccggcgc
      901 acctattctc accccctgct ctacacgagg gaggctggcg gcaccaggcg aggataggcc
      961 attctgtgca tcggaggcgg agaggggctg gcgatcgtcc tagagcggat gtagtaacgg
     1021 acggtagccc ccgaaagagg atactcttaa ctggcatcgc cggcggtgca gggaatcttc
     1081 gtctgctacg ccatcaccta cggcttcact gatggtctta tcactcgctg atatcaggct
     1141 agcgcgccac agtgccacga ggtagtccgc cacctcagaa aagacgaata tccctatttt
     1201 tctcgcccgg tcactgggtc aacgcttgga tctcggctgc ggctcgcaat ccactttcca
     1261 ccgctccatt catatatcct tgccaacgca ggctggtctg ttcaccggca aagaaaattc
     1321 gtccacaagg acgagccagc caggagcgga gtgtcggatc atacctcggg ccaaagacag
     1381 cgtagccgcc ttctacccac ggatcctgcc cccacgtgac ggaacgcatc gcaaccaact
     1441 ttttgcgtgt cttgccgagc cagtcgagcg ttcgcaccag accggctgcg tcggcggtcg
     1501 ataagagttt ctgcgtggcc tcgcttgccg tccctccggc catgaggcat aaaatcccgc
     1561 tcttcccctt ctgttgttcg tttgcatccc acactgcacc gatcggcaga ttcgtcccgt
     1621 acgccaacgg ttgattccgt ttcttccaaa accggtggtc aaattgcaac agcgttttgg
     1681 tcaccggtcc gtacgccaat gtcgagatgg cagcagcctg ttcacgaggc aaagcgggac
     1741 ggatgtcaag cgtgcgcact ttcgtggctg gcagtgcgag aatgagataa tcggatcgaa
     1801 gccacgattg attgttgccg gcacggatgc gcacacgaac gttggtaccg gattgggata
     1861 ctgccactac ttcatggcgt attagcagac gatcgcccag gatcgaagca agcgcctcag
     1921 gcagccggtc gtttccgcct gcgatccggt acatacctcc ctttcccgga ctgccggcgg
     1981 ccacctgatc gaccaaactg agcagcgaga ggttcactgg atcggccagg aaaaatccgc
     2041 gcaagccgac cagcatgtga cgcaactcgt cggggagcga gacttgctcc atccagtcag
     2101 caacagacac ggaacccaat ttcttggcca ctgctccgtc ccatcgctgc tcactcaatc
     2161 gataggcatg aatcagagga gccaggtgat gcgtcaaccg tttccagatg ccgtcgccag
     2221 agacaggccg ccggactttt ccatcgcatg acagcgcaaa ggaaaagcct gcacgtaata
     2281 ccggaacgag tttcaaattg agactgcgga ccaaccgaca aatctcttcg tgctcttcat
     2341 caatgaaatc ggcgcccgct tctgcatact gtcgctcgac aaacccgttc ctgatcgtca
     2401 agacccttcc gccgacccga ccctgccctt ccagcaccgt cacggttgcg ccttgtctct
     2461 gcaactccac agcagcggtc aaaccggcca gcccggctcc gacgacgatg acggacttcc
     2521 cacggagaga tcgaatcatg gtgtcagctt aacaggagga gaaagtcacg aactagatgg
     2581 ggcaggaaat accggtctca atgagattta tgagggaaat tgtggtggtg cagtttagct
     2641 atgtcggcat aggcattttc ctctgcttag cggtcacctt ctttcactga cgggttgatt
     2701 agttggtgac acgagacctc gcgtcatgaa cgccacaaaa tggtttggca atttcaaatt
     2761 taccccaaag tagaacagcc ctaacggtga cctattagct ggcgggggga tcttcgaatg
     2821 gatgacgcaa ctggttgatt ccctcccggc aatagccaag cggcaaccat cggcaggtgc
     2881 cgcagatgga aggcagatca tcaccaccca ccgaacgccg aatacgctcg agaatctctc
     2941 gccaacagac atgactcccc gcctccagac caagcttctc aagcaccaca cgatcctggt
     3001 cacgcatctc ctcctcagac tggtcaccgt tcaaggtgca tccggacgtt tgctggtgag
     3061 gacaggcgcc gcagacaaca tcgggggagg caagaacctc gacccagctt tctggatgct
     3121 cggtcagagc tcggtgaacc gaagccatgt tctcgacaaa ccctggacta tatccctcgc
     3181 cgcggaagcc ttgtagacac agcaaggtgt gcgccctgag cctggttggt gaggatccgt
     3241 ccttgagcaa cgagagctct gtcatcaagc tgttattttg tcatccggga ggagtatcag
     3301 tcaaagcatg gcttcaagac cccgctcgcc atgaatgact tgtccagcca aggacttgtc
     3361 cagcgaggcc gaggtcgctc tgcgctgaag cctggttgaa accttacaga gacccgcccg
     3421 acgccgtcct gacgaggtga tactcacttt ggaccatgcc ccccggataa gcacgtgtcg
     3481 caatatgact gaggcggacg tcgtgcagga gcgagccgaa gagcggaatt ccctcgccaa
     3541 tgagcacagg cagacggctg atgatcaaac ggtggatgag tccggcacgc aagaattgct
     3601 gaatggtgat tcccccgtcc acatacagcc ggctagcacc gcgcgcggtg agcatggaca
     3661 caatctcagc tggatcccca gccgtcagct caacgacgcc tccgcgcgat cgagcaacag
     3721 agagatcaag cggttgatgg ctcaagacga ccactcgctt ggtgccgtaa taccacttgt
     3781 cgaaggtcaa cactttctca aacgttctcc ggcccataac aatcgcatcg atgtcggcaa
     3841 tgaactcgtt gtacccgtac tcggcgctgt cgccaccgcc ctcgcccatc agccagtcga
     3901 gatcgccgtt gagccgcgcg atgaacccat cgagactgac gcccacaaag actgcgcact
     3961 ctggcacagc atgatcttgt ttcatgcatc acccttaggc tcttaagtcg ccatccatct
     4021 tttcttcatc gcttcctggg aatgcgagtg cggctatctt tcgaggtact aatttgggat
     4081 ctccgcttcg acgagtttcc ccagaaggat cagcgattct tgccatccga gatagcatgc
     4141 ttctgcggga atgacgtcgg gcacaccttc ttgcacaata ttgacttccg ttccacaaga
     4201 gactttcttc aatgtgaccg tcacgcgaat ttccccaggc aaattggggt cgtcgaaatt
     4261 gtccgtgtag cgaatccgtt catgcggcac aagttcgaga tactggcccc caaatgagtg
     4321 gctcttgcct gtggtaaaat tggtaaagga catcttgaag gtgccgccga ccttggcatc
     4381 catgtgatgg accttccctg taaatccatt gggcggcagc catttcgtca tggcatccgc
     4441 atcgagaaac gcccggtata tccgctccgg tgttgcacga aacacgcgat gaagccgaat
     4501 ggtattgatc cccacgacga tttctccttt ccatagccta aattcacgac ttttggctca
     4561 acgcttctcg tactcgtcgt gccggcgcca ccagacgcct gtttcgttgc gtcccttagg
     4621 cgcgcggtcg agccacggat acatgctcca gatagcgtcc actccgcgtg aatatgtgga
     4681 ataggtgtgg tagacgacgc catcctcgag cgcgaacgcg ctcatgccag gacgatcacg
     4741 cgagtacgtg gacgcatcgg ttccgcaggt ggcccctagc tgggcaacag gcaggggggc
     4801 cggtgtcgcg tccatcgcgt gaccgccgcg ctcgtaattg tattcgatca tccccccctg
     4861 ctgctgttcc acggtaaacg acacgttgaa atcgaagttg aagtcgctgc cgactgacga
     4921 tgcccaggga aatgtccatc ccatccgccg cttgtacgcc tgaagcttgg cgagcggcgc
     4981 cctcgacacc gcccagagca tcacgtcgtg attggcgagg tgcacggtga acccgttgaa
     5041 gccgtccgcg atcgcggagc aggacggaca ccctgccgtc cagtcaggcc caaacataaa
     5101 atgatagacg agcagctgcg aacgccctcg aaagaggtcc gccagcgagg cgcttccctc
     5161 gtcggtctcg aatcgatact gcttgtcgat ccgcacccac ggcaacgcct ggcgctgtcc
     5221 caccaccttg tcgctctgcc gcgtgagctc cttctccgac ttgagcagca cgagccgcgc
     5281 agcgagccac tcttcacgtg ttccggtctt gtgtttcttc atgggtatct ccttccttga
     5341 ggctctcttg gtcacaccta taacacttgt tcatcgcgaa cgctgtgaag gtgccgaccg
     5401 aagttgatcc ggtcacaatc agcactagac ttgtcaggca tgccggacac atgagtgatt
     5461 cctcacaatg aagctgcagc acgactgctg cctggaaagg ccctgacagg ccgcacctcc
     5521 atggtcccga accgcaccga cggaaacttc gaggcaaact ggatcgcctc gttcagatct
     5581 cgggcactga tcaggaaaaa tccaccgagc tgctctttcg tttcagcaaa gggaccgtcg
     5641 gtcacggaca acttcccgtt tcgaactcgt acactcatgg ccgtctgtat gggttcgag
//
LOCUS       DATIFR010000223         2697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1879242, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000223 DATIFR010000000
VERSION     DATIFR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879242"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1430
                     /locus_tag="VLD60_12670"
     CDS             222..1430
                     /locus_tag="VLD60_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB45860.1"
                     /translation="MNDSRVNQRGVIDRWLIPSLVAVGIAAGIIACGNKQEEPRAAKP
                     DTAASQSGLLRLTPEESSRMALEVVPVARGQLRVPREFPATVQSNENELAEVTTLIRG
                     RVVKVHVDVGQDVKKDTLLAMLHSTDLGVAEGAYLKASAKLHEAELVYERARDLHEHK
                     AVSLAELQRREAEMKTARAEARETQGRLQLLGVTRQEIERLEREHTIKADVPLRAPFD
                     GRVIMRNITRGEVVETNQKFFTVADLSDVWAVGNVPEKDVQYIRKDQKVDVIVSAYPH
                     AIFPGTITYISDVLDPATRTMRLRVTVPNPDRVLKPEMFATVRVYAAPTPEALTVPLA
                     AVQNGPTGKIVFVQRGTNEFEVRTVKLGSEQGEVVIVLDGVSAGEQVVTKGSFVLKSE
                     MERHKIEPTP"
     gene            1427..>2697
                     /locus_tag="VLD60_12675"
     CDS             1427..>2697
                     /locus_tag="VLD60_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB45861.1"
                     /translation="MIASLLEFSLRQRILVLGLACLFSVAGLFAFQSIPIDAYPDVTN
                     IQVQVLTEAPGLSPVEVERFITYPLELQMTGLPGLAEIRSLSKFALSQITVVFNDDVD
                     IYFARQLVLERMMAAKERLPEGLDPVMAPVSTGLGEVYQYYLEGSQVAETDPKIVQAE
                     LTDQRTIQDWVLRPLLKGVPGVIDVNGMGGFVKQYQVLVDPAKLRKFDLTIHDIYEAV
                     AKNNANAGGNVLERHAERAIVRGLGLIKTVGDIESIIVKESGGTPVFVRDVAEVRIGH
                     AVRHGAVILNGEREVVSGTVLMIRGGNARQVVEAVKVKVEDLQQNHVLPAGTKILPFY
                     DRIELVTAAIHTVRDALIEGIVLVVFVFFFFLGHVRSAIVVTASLIVTPLITFIVMQR
                     VGLSANLMTLGGLAIAIGEIADGSLVVVENV"
BASE COUNT          569 a          745 c          839 g          544 t
ORIGIN      
        1 agtatctcat aatcgataga gaaaatgacc caacgagtgt tgtactgcca gcccggcgct
       61 gttgcgtttc accacggtac tgatttaata ccgtggcaaa aggctacgga cggcttggtt
      121 ttcatggaaa aactgcgctg attctgaggc atgccccatg ctgtggtatt gaggatagag
      181 gagcgtgtcc ggactcagct actgaattgg gagtcgagac cgtgaacgac agtcgggtga
      241 atcaaagagg agtcattgat cgttggctta tcccaagtct cgtcgcggtc ggaatcgcgg
      301 cggggattat cgcttgtgga aacaagcagg aggagccccg tgctgccaag ccggatacgg
      361 ctgcttcgca gtccggtctc ttgcggctga ctccggagga atcgtctcgg atggctctcg
      421 aagtcgtacc ggtggcgcgg gggcagctgc gtgtgccgcg ggaattcccc gctactgttc
      481 aatccaatga gaatgagctt gccgaggtga ctacgctcat tcgtggccgg gtggtgaagg
      541 ttcatgtgga tgtcgggcag gacgtaaaga aagatacctt gttggctatg ctgcacagta
      601 cggacctcgg cgtcgcagaa ggggcgtacc tcaaagcaag tgccaagctg cacgaagcag
      661 aactggtgta cgagcgcgcc agggatctgc atgaacacaa ggccgtgagc ttggccgagt
      721 tgcagcgccg cgaggccgaa atgaaaacgg cgcgggccga agcgcgtgaa acgcagggtc
      781 gtctgcagtt gctcggcgtg acccggcagg aaatcgagcg gctggagcgc gaacacacga
      841 tcaaggccga tgtgcccttg cgtgcgccct tcgacggccg agtcatcatg cgcaatatca
      901 ccagaggcga agtggtcgag acgaaccaga aattcttcac cgtggcagat ctttcggatg
      961 tatgggccgt cggcaacgta ccggaaaagg acgtgcagta tatccgaaaa gaccagaagg
     1021 tcgatgtgat tgtgtccgcc tacccccacg ccatcttccc gggaacgatc acctacatca
     1081 gcgacgtgtt ggatccggcc acccgcacga tgcgcctgcg agtgacggtg cccaaccccg
     1141 atcgggtgct gaagcctgaa atgttcgcca ctgtccgtgt ctacgcggca ccgacgccgg
     1201 aggcgctgac tgtaccgctc gctgcggtcc aaaacgggcc gaccggcaag atcgtgtttg
     1261 ttcaacgggg gacaaacgaa ttcgaagtac ggacggtcaa actgggaagc gagcagggag
     1321 aggtggtgat tgtgctggac ggagtgagcg ccggtgagca ggtggtgaca aagggttcct
     1381 tcgtcctcaa atctgagatg gaacgccata agatcgagcc gacgccatga tcgcctccct
     1441 tctcgaattt tcgctgcggc aacgaatact ggtcctgggt ctggcctgtc tcttttccgt
     1501 cgccggcctg ttcgcttttc aatctattcc gatcgacgcc tacccggacg tgacgaacat
     1561 tcaagtccaa gtgctgaccg aagcccccgg tctctcgccg gttgaagtcg agcgattcat
     1621 cacctatcca ctcgaactcc aaatgaccgg tttgcccggt ctggcggaga tccgatcact
     1681 ttcaaaattc gcgctctccc agattacagt cgtgttcaac gacgacgtgg atatctattt
     1741 cgcccgccag ttggtcctgg agcggatgat ggccgcgaaa gagcgattgc cggagggact
     1801 cgatcccgtc atggcaccgg tcagtaccgg actgggagaa gtctaccagt attatctgga
     1861 aggctcgcag gtggcggaga ccgatccgaa gatcgtccaa gcggaactca cggaccaacg
     1921 gacgatccag gactgggtgc tacgaccgtt gctcaagggc gttccgggcg tgatcgatgt
     1981 gaacggcatg ggcggcttcg tgaaacagta ccaagtcctc gtcgatccgg ccaagctccg
     2041 caaattcgac ctgacgatcc acgacatcta cgaggcggtg gcgaagaaca acgccaatgc
     2101 cggcggtaac gtgctggagc gccatgccga acgggcgatc gtgcggggac tcggcttgat
     2161 caagacggtc ggcgatatcg agtccattat cgtcaaggag tcaggcggga cgccggtctt
     2221 cgttcgtgat gttgcagaag tccgcatcgg ccacgccgtt cgccatggcg cggtgatcct
     2281 caacggcgaa cgggaggtcg tgtcgggcac ggtgctgatg attcgcggcg gcaacgcgcg
     2341 ccaggtggtc gaagcggtca aggtcaaggt ggaggatctc cagcagaatc atgtcctccc
     2401 tgcaggtacg aaaatcctgc ccttctacga tcgcatcgaa ctggtgacgg cggccattca
     2461 caccgtacga gatgccttga tcgaagggat cgtgctggtc gtgttcgtct tctttttctt
     2521 tctcggccat gtgcgcagcg ccatcgtcgt gacggcctca ctcatcgtca ccccgctgat
     2581 cacgttcatc gtgatgcagc gggtggggct ctcggccaac ttgatgacgc tcggcggact
     2641 cgccattgca atcggcgaaa tcgccgacgg gtccctcgtc gtcgtcgaaa atgtcta
//
LOCUS       DATIFR010000224        33534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2837345, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000224 DATIFR010000000
VERSION     DATIFR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 33534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837345"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VLD60_12680"
     CDS             <1..1705
                     /locus_tag="VLD60_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSB45862.1"
                     /translation="SKSSEQQLDRDALAALYEETFKNLEEGTITEGRVVALTKDKVVV
                     DIGYKSEGMIPSDQFSNEELQNIKIGDRLQVYIEECEDADGNLVLSKEKADKMKIWEE
                     LEKLYKDEKSIDGKVVSRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGRTFPMKI
                     IKINHRRGNVVVSRRVLLEETRDKKRQTTLATLKEGQLIQGTVKNITDYGAFIDLGGI
                     DGLLHITDMSWGRVGHPSEMFNVGDRVEVSVLKYDRETGRISLGLKQKSADPWTNVAG
                     KYPIGTRVKGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVAIGDQVDA
                     AVLNVDPASRKISLGMKQTAPNPWDMVEGKYPIGTRIEGKVKSLTDFGAFIGLDEGID
                     GLIHISDMSWTKHIKHPSEIFKKGQKVEAVVLRIDKEKERLSLGFKQLSRDPWDDEIP
                     ARYRVGDSTTGKVSKVADFGIFVELDGGVEGLIHISEAGLDQSVKLEEKFKLQDEVTA
                     KIIKVDREERKIALSLRDHQQDSDRRQVDEYHAAQGAIDQSLGRAAKQSRKRGSADDE
                     R"
     gene            1766..2662
                     /gene="sppA"
                     /locus_tag="VLD60_12685"
     CDS             1766..2662
                     /gene="sppA"
                     /locus_tag="VLD60_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSB45863.1"
                     /translation="MAEDTQRKPTQRSWFRKILLLLGIGIGLLILVNLLFPDLDLSME
                     DRIALIRVEGVILDSQSTVMELKRFAENPSVKAIVLRIDSPGGGVVPSQEIYDAVQRV
                     RNKNNKAVIASMGTVAASGGYYIAAATDRIIANPGTLTGSIGVIMETANVEGLLKKIG
                     VEGIVVKSGKFKDVGSPLRKMTDEEQALLQSVMDDVHKQFIDAVAEGRAMEVATVQAF
                     ADGRIFTGRQAKEAKLVDELGNLEDAIQLAADLAGIEGEPKVVEPRRRFSLRELIESR
                     LSGIFPKLDFYSGVSLKYLMAF"
     gene            2715..2993
                     /locus_tag="VLD60_12690"
     CDS             2715..2993
                     /locus_tag="VLD60_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSB45864.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCVRDSLKNGDKTE
                     IRGFGSFRLRSRRMKEGRNPKTGATVAVPAKKVPFFKAGKELKELLNQ"
     gene            3016..4017
                     /locus_tag="VLD60_12695"
     CDS             3016..4017
                     /locus_tag="VLD60_12695"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSB45865.1"
                     /translation="MSASESDDAVVTEVRWTEGALKRMERAPMFLRGMVRRLAEKKAR
                     ELGYAEITETTLDQFKSQMMGGMGGASGMAEAAEAMAQGRLPWTAAAKERLNTVPEFM
                     RGMIRQIAEEVAKEGGHMEVNVDLFEKVEAMGDMREAVAAPLPWTDVALAKLQEKLKQ
                     SPPIAIEFVTDMLKRDTEDLAREQGIPQIDEQVLAQLWDAPQARVAWTDEAWKRLQTS
                     PDFVRSGIRKAAERRARKLGLKEIDSDHLTTFRNQAMMKAVKRIRSFGYNELTFDAFE
                     TALEKTKRLQGNEQAEKRLQEIREHFADPDTKKPEGGTLGAELMDRFRRYLKGEGSL"
     gene            complement(4129..4449)
                     /locus_tag="VLD60_12700"
     CDS             complement(4129..4449)
                     /locus_tag="VLD60_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45866.1"
                     /translation="MSKTVKIDITMYGIAEVLHWCHDRNKGRIAGVDTAAFQKMQALM
                     AEKPQSGDYFTLDQFWKKKVTLELTEEEVALIDRCLYDIPNFDNEPLPQIRHKFWPQQ
                     AAAR"
     gene            4693..5946
                     /locus_tag="VLD60_12705"
     CDS             4693..5946
                     /locus_tag="VLD60_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB45867.1"
                     /translation="MTKRYAARLLFLWLCRSALLFSLPSLFVLEACSKQDPYVPPDLF
                     YYFASYKVGKNPTTVTPVDINQDGITDLVTTNMSSNTLSILIGNGDGTFRDQVQLHVC
                     QEPRSLATGLFNSDVYPDIVLACSGSDQIAVLLGRADGKFDEGPQYPVHRTPIAIACD
                     DINGDHIADLVVALRNDKVKVFIGLGNGEFRHGAQYEYGDTPTSVALSDLNADGKVDL
                     VVTNGGPMSNAISVWVGNGDGTFRQPTDYRSGKRPLGVSFADFNNDRILDLLVINGER
                     DSFSTFLGNGNGTFQAGHDSGADAGPNFGLTRDFNGDRLADVAIVNLQSSDLSILFGR
                     GDGTFEYPPRNYRTKAGPFAVAVFRATTKEAEEPGLVTADNGNGTVSIFLHRGLKSGV
                     SPKPVVMSGEPAHTEAGARSGRSGE"
     gene            6037..6237
                     /locus_tag="VLD60_12710"
     CDS             6037..6237
                     /locus_tag="VLD60_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45868.1"
                     /translation="MRSFWWWFGIGVLLTLSVLMVQGGVRDLFDGSHQSGGANPYLSF
                     GTTIFGGGLLAGCLALVMNRIK"
     gene            6265..6453
                     /locus_tag="VLD60_12715"
     CDS             6265..6453
                     /locus_tag="VLD60_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45869.1"
                     /translation="MNCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVIDSGFRGAKVRESFTL"
     gene            6474..7397
                     /locus_tag="VLD60_12720"
     CDS             6474..7397
                     /locus_tag="VLD60_12720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSB45870.1"
                     /translation="MIMILSRSHASQRVMYSVVTILCAIMFGSVAVLTPSVAHAKRVG
                     GKSRHVHTVSEESQSLPWRRVPAHSILLKELNSGRVLYEHEPGKRLSPASLTKIMSAL
                     VILEQGRLDDLVTVSPVAARAHKTHLRLKAGQVFKLEDLLKGMLIVSANDACLAAVEH
                     VGGDEAQFVSLMNAKAAALGLADTHFSNGCGFDGPDHYSTAEDLATLSVIALQQPKFR
                     ELVREERAMIMPVNGTHAYVLHTTNRLLGRIPGVEGIKTGFTSKAGRCLIAKVSQNGS
                     DLLLVILNSSRRWNTATSLINYGLRASGSPQ"
     gene            complement(7536..8069)
                     /locus_tag="VLD60_12725"
     CDS             complement(7536..8069)
                     /locus_tag="VLD60_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45871.1"
                     /translation="MKEMLRVFLLTTVTVAGVGLWASSGFGEPYELSKNDVMDPKAIK
                     SPEVSLFGVKLGDPEAKALDALVNEKIPGIKVEQEALFIFLLDQRKPTGPMAGVRVQD
                     GKVDLIFVNNRFAYKTRGLLRNVLNSESPEDIRKLLGQEDYGDENVMGAVLAYDKQGF
                     VINYLGKDVNIEFSPVH"
     gene            complement(8232..9176)
                     /locus_tag="VLD60_12730"
     CDS             complement(8232..9176)
                     /locus_tag="VLD60_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HSB45872.1"
                     /translation="MELRHLRYFLAVAAAQNFTKAAETLHVSQPSLSVQVRDLEDELG
                     TRLFDRLGRTVGLTQAGMLFRDHAERALREVEQAAQLIRELDGAQRGRLVVGTLATVN
                     SYLIPPLVSQFKQRFPAVHLQVHSQPSAEIVEGILANRLDLGLCLLPVSQDHLTTVPL
                     FEERLVLVAPARHRLESSRIKMRDLAGLPLVLMPADYCLRKMVEEECAHAHVTTQVVL
                     EMTSPEGILQAVTEGAGFTILPELYVRQRLAHTKLRIIDLYDPIPRHSVGLAYHANRY
                     QNLAAKEFGNLCRSIMASLIEESLSPRSRQASVDAKRR"
     gene            9370..9822
                     /locus_tag="VLD60_12735"
     CDS             9370..9822
                     /locus_tag="VLD60_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HSB45873.1"
                     /translation="MMVWHSRILGYVMVCGILLVVTGCAGTEQAAGKSLYDRLGGKTA
                     ISAVVDQFVGNVAADSRINGRFATTDIPKLKGHLVDQICMATGGPCTYHGRDMRTTHA
                     GMKISNRDFGALVEDLVKALDTFKVPEREKGELLGLLGPMKKDIVEVP"
     gene            complement(9876..10169)
                     /locus_tag="VLD60_12740"
     CDS             complement(9876..10169)
                     /locus_tag="VLD60_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45874.1"
                     /translation="MDRETLHKLWDAHKQEDWPRVASQFEGPLMTLDTVISGCVVYFL
                     DSQDGLDEQRRGIVGDCLSDLESMEQELEEECRPYFQRLRELGTLLLITPHSS"
     gene            complement(10188..10577)
                     /locus_tag="VLD60_12745"
     CDS             complement(10188..10577)
                     /locus_tag="VLD60_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45875.1"
                     /translation="MKEYALACLAGVWLADGVALLTAPRVVMDRVREIVQNKPEIFRW
                     QILAVAAGIALLILGFDLPYGLLWIIIGIGMMLKGLFLWLGPGTLRQHFLDWCLTREN
                     VDYRFWGLGLCTLAVLLLHTLGWIGRP"
     gene            complement(10628..11893)
                     /locus_tag="VLD60_12750"
     CDS             complement(10628..11893)
                     /locus_tag="VLD60_12750"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSB45876.1"
                     /translation="MSSLRELNTATFRLAVAQFDQAAEAMNLDPNLRERLKFPQRSLI
                     VSIPVRMDDGRVEVFTGYRVQHDSSRGPSKGGIRYHPDVNLGEVAALAMWMTWKCALA
                     DLPYGGAKGGVAVDSKQLSRAELQRLTRRYASEIFPLIGPDQDVPAPDVGTDAQVMAW
                     IMDTYSQQVGYSVPGVVTGKPLSIGGSLGREEATGRGVVYVTFEALRRLNLDIRNTTV
                     AIQGFGNVGSHTARIMHESGARVIAVSDVQGGIYNSSGFDVPTLLARYRETGRSLKDT
                     KLGDHITNDELLQLDCTVLVPAALSEQITERNASKLRCRILAEGANGPTTLEADRIVQ
                     DKGIFVIPDILANSGGVIVSYFEWVQDAQRFFWKAADIQDRLRDLMTSAFQRTIEFAT
                     TKRTSMRMAALMSGIDKVAQAHLHRGLYP"
     gene            complement(11938..12654)
                     /gene="lipB"
                     /locus_tag="VLD60_12755"
     CDS             complement(11938..12654)
                     /gene="lipB"
                     /locus_tag="VLD60_12755"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSB45877.1"
                     /translation="MSFARQNPQPGTLVRFRHPVPYMESWGLQQQLHAELLSGAGKDS
                     LLLLEHQPVYTMGRRTESAHMGIGETALKQTGASVYAVNRGGSITYHGPGQLIGYPIL
                     QLAHYATGPKTYVQLLEEVLIRTLTCWGIRGHRVDRSPGVWVRDDQGEAKIAAIGVRL
                     EQGITLHGFALNVNLDLTPFSLITPCGLAQSRTTSVANLLQSEIPLERIADQIAETFS
                     TVFNIAWTHITVDSMASRAC"
     gene            complement(12705..13757)
                     /locus_tag="VLD60_12760"
     CDS             complement(12705..13757)
                     /locus_tag="VLD60_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB45878.1"
                     /translation="MTPSSTVPSPARTFFTLTFLCFLGIPLAWADSGTGTATSHDKPL
                     SVPEVVKKVRPSVVTILTRGVPASPSQAQSGSGSGVIIDESGYILTNNHLVTGIKSIV
                     VGLSTGRLTPGRVVARDFLLDLALVKINAQDLVPATLSPSPDLEIGESVVAIGNPLAL
                     KGGSTVTVGVVSALDRSVLTPDGETLYDLIQTDAAINPGNSGGPLVDLAGRVIGINVA
                     IAPSAQAISYAISMEAIYPHIQSMIVRGSIYRPDLGFTPITITPSIMASFGLEGDRGV
                     LALQVDAAKPAAVGGLQNGDIITAVDQHQIFNVGDFWHAVLRVGDQPTVQLTLHGKSG
                     PATLQLPKPHPIKSPQ"
     gene            complement(13877..15133)
                     /locus_tag="VLD60_12765"
     CDS             complement(13877..15133)
                     /locus_tag="VLD60_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB45879.1"
                     /translation="MKNDLFSADEPEKQSPEMKDADADHSEDSDLLDVIGRDSTEEKQ
                     LQDAIKPVKRQSMADGPFLLESLYFQSFGERALLTREEETALAKRIDQGSSIIRTTLR
                     HANRLLSGAKRSEAVIEAQHTFQTVRRLSGLSATALDTAEQSLSSLIKLGSGPQKLPA
                     NVVKQLKSALTTIREGRTTLEQAKDELVRCNLRLVVDVAKHYTRRGLTLLDLVQEGNI
                     GLMKAAERYQYRKGFKFSTYATWWIRQGITRALADQSRTIRIPVHQTEASHRILRVTR
                     RLGQQLGRPARLEEIANVLRMRPERLHETVQAFQEPVALEHPVGDGGTEFGELLPDLQ
                     AVPPDAHVHRTEMAHQLERILGTLTPREQTVIRLRFGIGYDQPCTLEQVGQNLSVTRE
                     RIRQIEAKALKKLKTPAVKEMFAAIQ"
     gene            15584..16453
                     /locus_tag="VLD60_12770"
     CDS             15584..16453
                     /locus_tag="VLD60_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HSB45880.1"
                     /translation="MSHFRNGERIHLVDKKGRQYALTLKAGDTYQLSGHKIPHDELIG
                     KLDGSLVTLSGEKPMIALRPTFGEYVLKMPRGAQVLYPKDLALIPMWADVYPGARVFE
                     AGTGSGALTMALLRAVGPRGLVVTYEAREDFARMAMTNIERYMGTMSNLVPFRKNAYE
                     GIDLLDDGLPFDRLVLDLPEPWQVVPHVALVLRSGGMYLSFVPTVPQVQRTVEALERT
                     GVFGMIETFETLLRTWSIQGRSVRPDHRMVAHSGFLTVARKVESGFWTARQASSLGSN
                     DMSDQESEEDKAS"
     gene            16450..17211
                     /locus_tag="VLD60_12775"
     CDS             16450..17211
                     /locus_tag="VLD60_12775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB45881.1"
                     /translation="MNRLEGKVAVVTGGNAGIGEAIAKRFAEEGASVVVTGRRQRELD
                     RVVSVIRRSKGEALAVAGSVADEAHAQNVARRALDSFGRVDILVNNAGIGDFGKRLHE
                     IDDATWANVLDVNLTGVFRMTRAIIPQMLKQGRGAIVNISSIASLVGIATLSAYAASK
                     GGLDALTRALAIDYAKDGIRCNVVNPGLIDTPMAAPLMADPERLKPILAQYAISRPGT
                     TEEVANMVLYLASDEAAWVTGGTFTIDGGMTIYKG"
     gene            17219..17566
                     /locus_tag="VLD60_12780"
     CDS             17219..17566
                     /locus_tag="VLD60_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45882.1"
                     /translation="MTMQNTMAQASKAAVWRAGLTIVCAILLLVPGLFLVQAGEGTEF
                     PGVVLMVDQAAGKFAVKKDGGGTRFTFVANEKTKFSGSGLSSLKDLKKDDHVVVFYHV
                     QGSQYLATSIAKK"
     gene            17578..18432
                     /locus_tag="VLD60_12785"
     CDS             17578..18432
                     /locus_tag="VLD60_12785"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSB45883.1"
                     /translation="MDINAHTQFCGVIGNPVEHSLSPAIHNAAFQTLGLNFVYLAWRV
                     EAIGDAIKGLRALGNFRGASVTIPHKVATVPFLDDLESTARDIGAINTIVSDSGKLKG
                     YNTDATGALRALREGGVGLKGQRVVMLGSGGAARAIAFALAAEGAIDCLVLLGIDKKE
                     RNALAGDLRSKTAIAVDDRHLDEDSLKQVLSNAQVLIHCTPTGMSPKVEETCVPSSHL
                     HAELAVMDIVYNPRETRLLKDAKGAGCRTIPGLEMFLHQAAAQFELWTNRPAPTEVMR
                     AVLESRFS"
     gene            18429..18959
                     /locus_tag="VLD60_12790"
     CDS             18429..18959
                     /locus_tag="VLD60_12790"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSB45884.1"
                     /translation="MNIVLIGYRGTGKSTVGKLLAARLSRELVSTDKEIIKRAGRSIP
                     EIVAEHGWEYFRDLESDVCRDLAGRDNLVIDTGGGAILRQQNVDVLKRNGALVWLTAS
                     VETIASRIGGDTQRPSLTGKKSFVDEIQDVLQERTPKYQEAADHILPTDGRSVNQLLE
                     SLLGLLGSAVSRSIPL"
     gene            19008..19084
                     /locus_tag="VLD60_12795"
     tRNA            19008..19084
                     /locus_tag="VLD60_12795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19042..19044,aa:Arg,seq:tcg)
     gene            19446..19592
                     /locus_tag="VLD60_12800"
     CDS             19446..19592
                     /locus_tag="VLD60_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45885.1"
                     /translation="MNHVILHDVRAGFFLMVGFAMLALICAVAALSTVMRPVRWADRR
                     SAQF"
     gene            19777..20466
                     /locus_tag="VLD60_12805"
     CDS             19777..20466
                     /locus_tag="VLD60_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB45886.1"
                     /translation="MGDIMKASLGKSGLVAALGVILLASQGCSMKWLQSDGEDVAGTA
                     KSGSGSEWNGQGVNPNSPGMAQSGSQSELSGFSQNPSEEYLASGSGIASLSSSDMNAR
                     HHAELTKEEKAAIEAGLQDVFFGYDQWTLSDAGMEALNRDAKWLKEHPGAVMKVEGHC
                     DERGTTDYNVVLGDKRSKSARSYLIELGVSPKQVAIVSYGKERPFCLEHDEACYQQNR
                     RGHVLLSVKKK"
     gene            20622..20978
                     /locus_tag="VLD60_12810"
     CDS             20622..20978
                     /locus_tag="VLD60_12810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB45887.1"
                     /translation="MTARSQALKEERCTEKRQQPRFTAQFRSTFSGGQREGRGKTLDL
                     SDGGCRIETDEPIVVGAAFECRIYAPGLDWPLRIDEAQVRWVKGKTFGVQFVSIRPDE
                     RVKLKRVIANLQSESE"
     gene            21138..21680
                     /gene="msrA"
                     /locus_tag="VLD60_12815"
     CDS             21138..21680
                     /gene="msrA"
                     /locus_tag="VLD60_12815"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSB45888.1"
                     /translation="MVMTQEVATLAGGCFWCLEAVYDQLRGVVSVESGYIGGHVDRPT
                     YEAVCGGRTGHAEAVRITFDSAVVSYRELLEVFFVVHDPTTLNRQGHDVGTQYRSAIF
                     YHNADQKQVAEDVIATFKKEKVYPNPIVTEVVPAGSWFEAEPYHQEYFARNPFQGYCT
                     AVVGPKVMKFRKLFSEKLKP"
     gene            complement(21692..22120)
                     /locus_tag="VLD60_12820"
     CDS             complement(21692..22120)
                     /locus_tag="VLD60_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45889.1"
                     /translation="MPPRQEDLQQSGPPLWSIGLVVALMLVVPVVLYSIAPPGPIREG
                     DTIFSDGVLKVPLAHPLLYESANFNGTCLVDPHDPLTVLQGPSDRPDGLILAKVQGKT
                     AIEWPFCPPQAEVLFVVPHSMQKTAFFPGAQSRLMEWWKR"
     gene            complement(22136..23002)
                     /gene="ttcA"
                     /locus_tag="VLD60_12825"
     CDS             complement(22136..23002)
                     /gene="ttcA"
                     /locus_tag="VLD60_12825"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HSB45890.1"
                     /translation="MMQTSSHLPAPPRLDEATEQLQTRLLRQVGQTISDYGLIEQGDK
                     VMVCLSGGKDSYGLLDLLLVLQRRAPIRFDLVAVNLDQKQPGFPAHILPEYLASVGIP
                     YHIEERDTYSIVKRLIPEGQTTCSLCSRLRRGHLYRVATELGATKIALGHHRDDILET
                     LLLNLLFTGKLKTMPAKLRSKDGRHLVIRPLAAVREADLARYAERRNFPIIPCDLCGS
                     QEDLKRKQVKEFLRAWERKAPSCTDSMFAALSNVAPSLLLDKRLFDFSSLRVAGAAEG
                     DEDAWLDQDNAV"
     gene            23061..23558
                     /locus_tag="VLD60_12830"
     CDS             23061..23558
                     /locus_tag="VLD60_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HSB45891.1"
                     /translation="MSHRYWPQSVLFVCTGNIFRSLAAEQALKTMLGAESSLHVGSAG
                     IDAKPQAVHEWVQTRLRLKGADPSGHVQRQLTIEMVQAADLVIAMGCNHQLFIRDRFG
                     HDVPLFNQVCFGRDEPIEDLHEVMPKWEQNLEEARAYVWSVIDSIWEATPALIQRLPS
                     LRSLK"
     gene            23712..24773
                     /locus_tag="VLD60_12835"
     CDS             23712..24773
                     /locus_tag="VLD60_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HSB45892.1"
                     /translation="MSRSGLREVTLSDIGRSEPAWVSWSDEKLLDLQICRLNVTLDNP
                     FLSGNIQQLYDELEAKQLRFRPHFWLSNEWFTPDGVPGIAIPFYLAHPRLAKLELAQM
                     LEVEGGTAEWCMRILRHEAGHAIENAYRIRRLRSRQQIFGRSSQPYPKYYSPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLTPDSMWAERYKGWPVLRKLQYMDSLMGGFVNV
                     PPIVTTKELIDPLHTLKKTLREHYERKRSHYGIARSSFYDPDLKRLFSDAPTHADRVS
                     AATFLNRFRREVRRKVASWTGEYQYTIDQVLEDMIQRCRELHLRLPLPEEQAKLDFTI
                     LLTVHTMNYLRSGRHKVAV"
     gene            24770..25792
                     /locus_tag="VLD60_12840"
     CDS             24770..25792
                     /locus_tag="VLD60_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSB45893.1"
                     /translation="MRRLRILVLMHKDLVPPEPLNGHDPLRAEWKTEYDVVTTLRKLG
                     HKVKPLGVKSDLGVIRSAVEEWKPHIAFNLLEEFDGVAVYDQNVVSYLELLHMPYTGC
                     NPRGLMLARDKVLSKKLFSFHRIPFPEFMVVPEGRTVKRPKWLSFPLIVKSVTEEASL
                     GISQASIVQDDEKLKERVAFIHQRVGTGALIERYIEGRELYVGVLGNGHLQVFPVWEL
                     KMDKLPEDARRIATERVKWSRKYQDKYGITSKEAKNLPEGKAEEIQHLAKRVYRALGL
                     SGYARIDMRLDEKGQLYVLEANPNPQIAHSEDFADSAEKANYPYKELLQELLNVGLRW
                     RPAKAA"
     gene            26033..26512
                     /locus_tag="VLD60_12845"
     CDS             26033..26512
                     /locus_tag="VLD60_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45894.1"
                     /translation="MTPREAASAVIAAMPTGLSDSQLQEYGIEATAERAQALTREMLS
                     LNLFWIFAAIEAHIPQKFQQAVSALVLHAVETGWGTTYPVGPVAWTTFLTEWKERTKR
                     YERLVKDGMSPLAISAEVGMWLEEQHIVGEEDRRYVLTLLIDSVPVESYGQLLQDVG"
     gene            complement(26525..27130)
                     /locus_tag="VLD60_12850"
     CDS             complement(26525..27130)
                     /locus_tag="VLD60_12850"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSB45895.1"
                     /translation="MGRLILLRHGESQWNLENRFTGWVDVPLSPRGIQEARNAGEKLR
                     GFTFNRAFTSVLTRAIETLRLVLETIGQSGIPIEKDKALNERMYGELQGLNKAETAKK
                     YGDDQVKIWRRSYDVPPPGGESLKDTADRVLPYYEQKIKPHLLKGDTILIAAHGNSLR
                     ALVMQFEQLSKEQVLELNIPTGAPLLYELDTTGKVINHRYL"
     gene            27478..28302
                     /locus_tag="VLD60_12855"
     CDS             27478..28302
                     /locus_tag="VLD60_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45896.1"
                     /translation="MTIPPSSEINWPALESWWIAMGDRLHVADRARSFFIKVRSDGQL
                     DRIVQVGGQVDYILFVLMRYWVPTVLPPPGRERLTKNDPDYWLESEHILKAAAARLRE
                     LKPLIELLTTPGPLSDAPSGKGVSPSPVAELELSSIVEGIGEAAGTYGGPDYTSIVKA
                     FDPVPLRQTQPFKHNKKNSAELWVVFLFREHFRSLGLGKDRYWPLVAPLCTAAGILNQ
                     NGQAFSPDELKSWWQKNWPRTYTQLEQKQDAPDSGGAAYAQDFGWFQAWIGWQRAR"
     gene            complement(28311..28952)
                     /locus_tag="VLD60_12860"
     CDS             complement(28311..28952)
                     /locus_tag="VLD60_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HSB45897.1"
                     /translation="MKSCAQCQQQNQDDSKFCYQCGTHLTAPAEQATPASTVHPTQSD
                     SDLWRAFIGPNADRYLEQFKKFGPEQTPKFALTWNWPAFLFVSFLWFLYRKMYLYAAV
                     YAIGPMASTYLTGDMTSGVVWSIVAGATANYVYYWHCREQIGTIKQQLWSEPAKQDVA
                     VKEAGGVQPYVIWVGVALYVLFSITMFKMIQDGALDGERLPTQPAKPAATTHT"
     gene            29097..30308
                     /locus_tag="VLD60_12865"
     CDS             29097..30308
                     /locus_tag="VLD60_12865"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HSB45898.1"
                     /translation="MPTEELKQGTEQYLMGTYARQPISIVRGRGTKVYDLEGREYIDF
                     VGGVAVNILGHGHPDLVLAIQRQAAQLIHTSNLYYTEPQVKLAQMLVDHSFADKVFFC
                     NSGAEANEAAIKLARRYSHDKYGPGRFEIITMKNSFHGRTMATLTATGQEKVQKGYDP
                     LLPGFLYVPFNHLAELDQTVSGKTAAIMLEPIQGEGGVHVADREYLKAVRELCTQKDV
                     LLIFDEVQTGMGRTGTLFAYEQLGVQPDIMTLAKGLGGGVPIGACLAKDEIAKAFGPG
                     SHASTFGGNPLACAAALAVFRVLLEGRVLDHARRMGDYLAKGLSEFKDRHHAVKDVRG
                     LGLLQGLEVEMNTKTVVSDCVRRGILVNATGEHVLRFVPPLIISQPEIDRLIEVLSQI
                     FNRQAAEGPAH"
     gene            30327..31298
                     /gene="argF"
                     /locus_tag="VLD60_12870"
     CDS             30327..31298
                     /gene="argF"
                     /locus_tag="VLD60_12870"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSB45899.1"
                     /translation="MKPSAQRTTGLSKDLLDVAALSKAEVESLLTLATRLKDKQRRGV
                     PHPVLQGKTLGLLFEKPSTRTRVSFEAGMNQLGGHALILPMGDIQLSRGESVPDTARV
                     LSRYLDGIVIRTYDHATVQEWAREATMSVINGLTDLSHPCQALSDLLTIKEKKGRLKG
                     IKVAYIGDGNNVANSLIEAAAKMGMTISLGCPAGYQPDQHVVDLARLEAEKTGALIEL
                     SHDPSIAAKEADVIYTDVWISMGREREQARRLKVLAPYQVNSRLVSRAKSDAIIMHCL
                     PAHRGEEISAEVLDGPQSVILDQAENRLHMQKAILTKLLGKGKEGKR"
     gene            31295..32506
                     /locus_tag="VLD60_12875"
     CDS             31295..32506
                     /locus_tag="VLD60_12875"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSB45900.1"
                     /translation="MSRAIKKVVLAYSGGLDTSVILKWLQETYQAEVIAFCADLGQGE
                     DLKAIKAKAQALGVKKVYVEDLRETFVKDYVFPMLRGNAMYEGCYLLGTSIARPLIAR
                     RQAEIALKEGAEAVSHGATGKGNDQVRFELTYTALAPGLKIIAPWREWTMRSRRELIE
                     YADRHGIPVTATKAKPYSMDLNLFHVSYEGGILEDPWEAPPDEIFQMTVSPEKAPNKP
                     LEVEIDYEAGNPVAVDGKKMSPANLLAHLNKLGGAHGIGRVDLVENRYVGMKSRGVYE
                     TPGGTILHVAHRGLESLTIDREVLHFRDSLIPRFADLIYNGYWFSPEREMVQTAIDEA
                     QKDVTGTARVKLYKGSCTLAGRKSKNSLYRLDIATFEEDEVYNQKDAEGFIRLNALRL
                     KIRAQRKKASS"
     gene            32506..>33534
                     /gene="argH"
                     /locus_tag="VLD60_12880"
     CDS             32506..>33534
                     /gene="argH"
                     /locus_tag="VLD60_12880"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSB45901.1"
                     /translation="MRRAKKRSRAESASGKAWEGRFREKTNRLVEAFTVSVDVDRRLY
                     AHDIQGSIAHCKTLARARVLTPGETRAIVRGLESVKTEFDRGRFRFLPQDEDIHMAIE
                     RRLTELIGPLGGKVHTGRSRNDQVALDVRLYLRDQLDLLLARLEEFQLVLVAKAKANR
                     TLAMPGYTHLQRAQPVLFAHHLLAYVEMLERDKGRLRDAKHRLNVMPLGSGALAGTNY
                     PVDRRYTARLLDFPAVTANSLDAVSDRDFMIEIAAALSIAMMHLSRLSEELIVWSSQE
                     FRFVDLPDGFCTGSSMMPQKKNPDVPELVRGKTGRVYGHLINLLTMLKALPLSYNRDL
                     QEDKPALFD"
BASE COUNT         7370 a         8874 c         9986 g         7304 t
ORIGIN      
        1 ttcgaaatcc agtgagcagc aattggaccg cgatgccttg gccgccttgt acgaggaaac
       61 cttcaagaac ctcgaggagg ggaccatcac cgagggccgc gtcgtggcct tgaccaagga
      121 caaagtggtc gtcgacatcg ggtacaagtc cgaggggatg atccccagcg atcagttctc
      181 gaatgaagaa ctgcaaaaca tcaagatcgg cgatcggctc caggtctata tcgaggaatg
      241 cgaagatgcg gacgggaacc tggttctttc caaagaaaaa gccgacaaga tgaagatttg
      301 ggaagaactc gagaagctct ataaagatga gaagagcatc gacggcaaag tggtatctcg
      361 gatcaagggc ggcatgatgg tggatatcgg cgtgaaagca ttcttgcccg gttcacagat
      421 cgatctgcat cctgtccggg atctcgacgg attggtcgga cggacgttcc cgatgaagat
      481 cattaagatc aatcatcgac gcggcaacgt cgttgtttcc cgtcgcgtcc tgctggaaga
      541 gacgagggac aagaagcgcc aaacgacgct ggcgactctg aaggaaggcc agctgattca
      601 agggacggtc aagaatatca ccgactacgg ggccttcatc gacctcggtg gtatcgacgg
      661 cttgcttcat atcacggaca tgtcctgggg ccgtgtcggg cacccatccg aaatgttcaa
      721 cgtcggcgat cgcgtcgagg tgagcgttct caagtatgat cgggagacag gccgtatctc
      781 gctcggcctc aaacaaaagt ctgccgatcc ctggacgaac gtggctggta agtaccccat
      841 cggcaccagg gtcaagggcc gagtcgtcag tctgacggat tatggcgcct ttgtggagtt
      901 ggagcccggc gtcgagggac tcgtccatgt ctcggagatg tcctggacac acgaagtccg
      961 ccatccatcg cgggttgtgg ccatcggcga tcaggtcgat gctgcagttc tcaacgtgga
     1021 tccggccagc cggaagattt cactcgggat gaagcagact gcaccgaatc cgtgggatat
     1081 ggtggagggc aagtacccca tcgggacccg tatcgaaggt aaagtgaaga gcctgaccga
     1141 tttcggagcc tttatcggat tggatgaggg cattgacggg ttgatccaca tttccgatat
     1201 gtcctggacc aagcacatta agcacccgtc agaaatcttc aagaagggcc agaaagttga
     1261 agccgtggtg ttgcgaattg acaaggaaaa ggaacggctg tcgctcggct tcaagcagct
     1321 cagccgcgat ccatgggacg acgagattcc cgctcgctac cgcgtcggag attcgacaac
     1381 cgggaaagtg agcaaggtgg cggatttcgg catcttcgtc gagttggacg ggggcgtcga
     1441 agggttgatc cacatcagcg aagcgggcct ggatcagagt gtgaagctgg aagagaagtt
     1501 caagttgcag gacgaggtga cggccaagat tattaaggtg gatcgcgagg aaaggaaaat
     1561 cgcgttgagt ctgcgcgatc accagcagga ttcggatcgc cggcaggtgg acgagtacca
     1621 cgcggcgcag ggtgcgatcg atcagagcct cggtcgtgcc gcgaagcaga gccgcaagcg
     1681 cgggtcggca gacgacgaga ggtaactttc tcagagggcc ggcgccgaga gcttcatgct
     1741 tcggcgccgg acaagagtga gacatatggc agaagacact caacgcaaac cgacacagcg
     1801 atcctggttc cgaaagatcc tcttgctgtt gggaatcgga atcggcctcc tgattctagt
     1861 gaatctgttg ttcccggacc tcgacctgtc gatggaggac cggatcgcat tgattcgtgt
     1921 cgagggggtc attctggatt cgcagtcgac ggtcatggag ctgaagcggt tcgcagagaa
     1981 tccttctgtc aaggccatcg tcctgcgcat tgatagcccc ggcggaggcg tggtgccttc
     2041 ccaagagatc tatgatgcgg ttcagcgtgt caggaacaag aacaataagg cggtgattgc
     2101 gtccatggga actgtcgcag cttcaggtgg ttactacatc gccgcggcga ccgatcgcat
     2161 aatcgccaac cccgggacat tgaccggcag tatcggggtc atcatggaaa cggccaacgt
     2221 cgagggtttg ctcaaaaaaa tcggcgtcga aggaatcgtg gtcaagagcg ggaaattcaa
     2281 ggacgtcggc tcgccactca ggaagatgac cgatgaagaa caagcgctct tacaatcggt
     2341 gatggacgac gtgcacaagc aattcattga tgccgttgcc gaaggacgcg cgatggaagt
     2401 tgccacggtg caggcgttcg cagacggccg gatcttcacc ggacggcagg ccaaagaggc
     2461 caagcttgtg gacgaattgg gaaatttgga ggacgcgatt cagctcgcgg cggacttggc
     2521 cggaatcgag ggagagccga aagtcgtgga gccgcgccgc cggttctcgt tgagagaatt
     2581 aatcgagtcg cggttgtcgg ggattttccc gaaactggat ttttactcag gggtaagtct
     2641 caaatacctc atggcgtttt aactcggtgg gagcgacgag ttagccgctc gtcgtagcga
     2701 aaggggaatc ggacatgacg aaggcccaga ttatcgagcg agtctccgag caggtgacca
     2761 cgttgacaaa gcgccaggcg gagatcgtgg tcaatacgat cttcgactgt gtcagggatt
     2821 cgttgaagaa cggggacaag acggagattc gagggttcgg cagttttcgg cttcgatccc
     2881 gccggatgaa agaagggcgc aatccgaaaa ccggcgcgac ggtggcggtg ccggccaaaa
     2941 aggttccatt cttcaaggcc ggaaaagaac tcaaggaatt gctcaaccag taaccggatt
     3001 gattgaagga ttatcatgag tgcatcagaa tccgacgacg cagtcgtcac cgaagtgcgt
     3061 tggaccgaag gcgcgttaaa gcgcatggag cgcgcaccga tgtttctgcg cgggatggtc
     3121 cgccggctcg ccgagaaaaa agcccgtgag ctgggatatg cggaaatcac ggaaacgacg
     3181 ctcgatcagt tcaagagcca gatgatgggc ggtatgggtg gcgcatccgg catggcggaa
     3241 gcggcggaag ccatggcgca aggccgtctt ccctggaccg ctgcagcgaa ggaacgactg
     3301 aatacggtgc ctgaattcat gcggggcatg atcaggcaaa tcgcggagga ggttgccaaa
     3361 gagggcggcc atatggaagt caacgtcgac ctctttgaaa aagtcgaggc gatgggagac
     3421 atgcgtgagg cggttgcggc tccgttgcca tggactgacg tggcattggc caagctgcaa
     3481 gagaagttga aacagtcacc tccgatcgcg atcgaatttg tcaccgacat gctcaaacgg
     3541 gacacggaag atttggcgcg cgagcagggg atccctcaaa ttgatgaaca ggtgctggcc
     3601 cagctatggg atgcgcctca ggcccgcgtt gcctggaccg atgaggcctg gaagcggctg
     3661 caaacctcgc cggattttgt gcgcagtgga attcggaagg cagcagagcg gcgagcccgc
     3721 aagctcggac tcaaggaaat cgactccgat catctaacga cgttccgaaa tcaagccatg
     3781 atgaaagcgg tgaaacggat tcgctcgttc ggctacaacg agctgacgtt cgacgcgttc
     3841 gagacggcgc ttgaaaagac caagcggttg caggggaacg aacaggcgga aaagcgatta
     3901 caagaaattc gcgaacactt tgctgatccg gacacaaaga agccggaagg gggaacactg
     3961 ggcgcagaac tcatggatcg tttccggcgg tatcttaaag gggaggggtc cctgtagccc
     4021 gttccctctt gtggctggct cgggacgcct ccagccaata ctgaccatgt cgcttttcga
     4081 gcgacatggt cagagttgtt ctagggttgt tggtcggaca acgaacgttc agcgggctgc
     4141 ggcttgttgc ggccagaact tatggcgaat ctgggggagc ggttcgttgt cgaagttggg
     4201 gatatcatag aggcagcggt cgatcaatgc gacttcctct tccgtcaact ccaacgtcac
     4261 tttcttcttc cagaattgat ccagcgtgaa gtagtcacct gattgtggct tttccgccat
     4321 aagagcctgc attttttgaa atgctgccgt atccacgcct gcaatccggc ctttgtttcg
     4381 atcgtggcac cagtgcagca cttcggcgat cccatacatc gttatatcga tcttcacggt
     4441 cttactcata gactcctcct tacgaaagac acacagtgta acccagatga tgcctgattt
     4501 tcaaagccct tccgcctggt ttctcaggct cactcacagc cccaccatgg tatcgccgcc
     4561 gtcttctcct tcgttgccag gggaaagcgg ccatcggtat actagcgagc ttttgaaggg
     4621 caagacaggc ggcggcctgg ttcatccgca gattacgcga ttgggggaaa ttcctttcgt
     4681 aaggagttcg gcgtgacaaa acgatacgca gcccgattgc ttttcctctg gctatgccgc
     4741 agtgccctct tattcagtct cccttcgctg ttcgttctgg aggcctgttc caagcaggac
     4801 ccctacgtcc cgccggatct cttttactac ttcgccagtt ataaggtcgg caagaatccc
     4861 acgaccgtga cccctgttga catcaatcag gatggcatca ccgatttggt gaccacgaat
     4921 atgagcagta acaccctctc aatcctcatt gggaacggtg atggaacatt tcgcgatcaa
     4981 gtgcagttgc atgtctgtca agagccaagg tcgctggcca cagggttgtt caacagcgat
     5041 gtctatcccg atattgtgct ggcctgttcg ggtagcgatc agatcgcagt gctcctcggc
     5101 cgtgcggacg gaaagttcga tgaaggtccg caataccccg tccatcggac accgatcgcc
     5161 attgcttgcg acgacatcaa cggagatcac atcgctgact tggtcgtcgc gctcagaaac
     5221 gataaagtga aagtcttcat cggattggga aacggagaat tccgtcacgg agcacagtac
     5281 gaatacggtg acacgcccac gtcagtggcc ctatccgatc tcaacgcaga tgggaaagtc
     5341 gatcttgttg tgaccaatgg gggccccatg tccaatgcca tttccgtgtg ggtcgggaac
     5401 ggagacggaa cctttcgcca gccgacggac tatcgctcag ggaagcgacc gctgggcgtg
     5461 agcttcgccg atttcaacaa tgatcgtatc ctcgacttgc ttgtcatcaa cggagagcgg
     5521 gatagctttt ccacgtttct tggcaacggt aatgggacgt tccaggccgg tcacgattct
     5581 ggagcggatg ccgggccgaa cttcggctta acgcgtgact tcaacggaga ccgcctggct
     5641 gacgtcgcca tcgtcaatct ccagtcgagt gatctctcga tactcttcgg gcgaggtgac
     5701 gggacattcg agtacccacc gaggaattat cggaccaagg ccgggccgtt tgcggttgcg
     5761 gtctttaggg cgacgacaaa ggaggccgaa gagcctggac tcgtgacggc ggacaacggc
     5821 aatgggacgg tttcaatttt tctgcaccga gggctcaaat ctggtgtctc tcccaagccg
     5881 gtcgtcatgt ctggtgagcc tgctcataca gaagcgggag cgaggtcggg gcggtcgggc
     5941 gaatgagcag tctgcttgac agtctgggac ccctcggcta gagtggccga caggagcggc
     6001 ttttgagcgg ctctgctgtt ccttgcgagg acatcggtga gatccttctg gtggtggttc
     6061 ggcatcggcg ttctcctgac tctgtccgtg ctcatggtgc agggcggtgt gcgagacttg
     6121 ttcgatggat cgcaccaaag cggtggagcg aatccctatc tctcgttcgg gaccacgatt
     6181 ttcgggggtg ggctattggc cggatgcctc gcgttggtga tgaatcgcat caagtaaacg
     6241 ggtcaccgca gctcggtttc tgttatgaat tgtctgaagt gcaaagggtt catgatggtc
     6301 gagcggcatt atacgctgat gaaccgacgt ctctatgcac gatgtttgaa ctgtggattc
     6361 tggattgatt tagccgatct ccttcgtttc ttccacaagg tgattgacag cgggttccgg
     6421 ggtgccaaag tccgggaatc ctttacgctc tgaacggaaa ggttccccat cgcatgatca
     6481 tgatattgag ccgctctcat gccagccaac gagtcatgta ttccgtcgtc acgattttgt
     6541 gcgccatcat gttcggctcg gtggctgtac tcactccatc ggtggcccac gcgaagcgcg
     6601 tgggaggcaa gtccaggcat gtccacaccg tcagcgaaga aagccagtca ttgccctggc
     6661 ggcgggttcc cgcccatagc attttgctca aagaactgaa cagcgggcgc gtactctatg
     6721 agcacgagcc cgggaaacgg ttgtcaccgg ctagtttgac gaaaatcatg tcggcgcttg
     6781 tcattcttga acagggcagg ttggacgatc ttgtaacagt gagccctgta gcagcgcggg
     6841 cgcataagac ccatcttcga ttgaaggcgg gacaagtctt caagctcgaa gacttgctga
     6901 agggaatgtt gatcgtgtcc gcaaacgacg cctgcttggc cgcggtggaa catgtcggcg
     6961 gggatgaggc tcagtttgtg tcgctgatga acgcgaaagc cgctgcgctg gggttggccg
     7021 atacccattt cagcaacggg tgcggattcg atggaccgga ccattattcg accgcagaag
     7081 acttggccac cttgagcgtt atcgcgctgc aacagcctaa gtttcgcgaa ctggtacggg
     7141 aagagcgcgc catgatcatg cccgtcaatg gcacgcatgc ctatgttctg cacacaacca
     7201 atcgtctgct cgggcggatt cccggtgtcg aaggcatcaa gacgggattt acttcaaagg
     7261 ccggccgttg cctcattgcg aaagtatcgc agaatggcag tgacctcttg ctggtcattc
     7321 taaattccag caggcgctgg aataccgcca caagcctgat taactatgga cttcgcgctt
     7381 ccggctcccc gcagtgaacc cccctctcca tcttacgttt ccccgctttt taggatgagc
     7441 ctgtacagct cggattagta aggaaggcgg aaccacatag cccccccctc gaccggaaga
     7501 gaacgcggac taggctcgca gctgatagag attggctagt ggactggaga gaattcgatg
     7561 ttgacgtctt tcccgagata gttaatcacg aagccttgct tatcataggc taagaccgca
     7621 cccatcacat tctcatcccc ataatcttcc tgtccgagga gtttccggat atcttccggg
     7681 ctttcactgt tcaggacatt gcgcaacagg ccgcgcgtct tgtaggcaaa tcggttgttc
     7741 acgaagatga gatccacctt cccgtcttgt acgcggacgc ccgccatggg gccggtgggt
     7801 ttccgttgat cgagcaggaa gataaagagg gcctcctgtt cgaccttgat cccaggaatc
     7861 ttttcattga ccagagcatc caatgctttt gcctcgggat ctcccaattt gacgccgaac
     7921 agggaaacct ccgggctctt aatcgctttg ggatccatga cgtcgttctt gctgagttcg
     7981 tagggttcgc cgaatccgga agaagcccaa aggccgaccc cggccacagt gacagtggtc
     8041 agcagaaaca ctcgcagcat ctccttcatc gactcctcct tgcttacctg gtgccaacgg
     8101 agcgttggga cgttagacgt gtttatccag cggggggatc atgcccattt gtggcattct
     8161 atcgaatggg ctgtagaact tgcaaggaac ggaagcctcg attggaggaa gaggctggtc
     8221 ccagcagagg atcaccgccg tttggcatca acggaggcct gccgagatcg tggtgatagg
     8281 gattcctcga tcaggcttgc catgatcgaa cggcacaggt tgccgaactc cttggctgca
     8341 agattctggt agcggttggc atggtaggcc aacccgacag aatggcgagg aatcgggtcg
     8401 tacaagtcaa tgatccgaag cttcgtatgt gccaggcgct gacgaacgta gagctccggc
     8461 aggatagtaa agccagctcc ttcagtcact gcttgcagga taccctcagg tgatgtcatt
     8521 tccagcacca cctgagtggt cacgtgtgcg tgagcacatt cctcctccac catctttctg
     8581 aggcaatagt ctgccggcat caacacgagc ggaagtccgg ccagatccct catcttgata
     8641 cgagatgatt cgagccgatg ccgcgctgga gcgactagta ccagtcgttc ctcgaagagc
     8701 ggaacggttg tgaggtgatc ctgcgaaacc ggcagaaggc agaggcccag atcgagtcga
     8761 ttcgccagaa tcccctcgac aatttcagcg gacggttggg aatggacttg aagatgcacg
     8821 gcgggaaatc gttgtttaaa ttgcgagacc agtggcggaa tcaagtagga attcacggtg
     8881 gccaatgtgc cgacgacaag ccggcctcgc tgtgcgccgt caagttcacg aatcaactga
     8941 gcggcttgtt ccacttctcg gagcgcccgt tccgcatggt cacgaaagag catgccggct
     9001 tgggtgagtc ccaccgtccg tccgagccgg tcaaagaggc gcgtgcccaa ctcatcctct
     9061 agatctcgaa cttggacgga aagagatggc tgagagacat gaagggtctc cgccgctttc
     9121 gtaaaattct gtgcggcggc gacggcaaga aaatatctca ggtgtcgcag ttccatggcc
     9181 ggtagaggcg taagttacat atagttaaaa gctatgcact gcattaattc aatatattgg
     9241 acctatgaat gctaccagcc tcatgctgca ccgtcaacag atgagtggtt tgcccgggag
     9301 tatcttgggg taagccaggc agattcattt tgagaacagc agcgacatgg ttgctgctta
     9361 tggaggtgca tgatggtgtg gcattcgcgg atcctgggct atgtgatggt gtgcggtatt
     9421 cttcttgtgg tcacgggctg tgcgggaacg gaacaggcgg ctggcaaatc actctatgac
     9481 cggctcgggg gcaaaacagc gatttcagca gtggtcgacc agtttgttgg gaacgtggcg
     9541 gctgattcaa gaattaacgg gcgatttgcc acgaccgaca ttccgaagct caaggggcat
     9601 ctggtggatc aaatctgcat ggcgaccggc gggccttgca cgtatcatgg ccgggacatg
     9661 cgaacgactc atgcggggat gaagatttcc aacagagact tcggagcgct cgtcgaggac
     9721 ttggtgaagg cactggatac cttcaaggtg ccggaaaggg aaaagggtga acttctcggc
     9781 cttcttgggc cgatgaaaaa agatatcgtc gaagtcccgt gaattgatct tggagggtga
     9841 gggtcgccat cggccctcgc ccgtgaccca gggatttagg agctatgcgg ggtgatcagc
     9901 agcagtgtgc caagctcgcg gagacgctga aagtatggac ggcactcttc ctcaagttcc
     9961 tgttccatcg actcgagatc cgaaaggcag tcgcccacga tacccctccg ttgctcatcc
    10021 agtccgtcct ggctgtcgag gaaatagacg acacagccgc tgatcactgt atccaaggtc
    10081 atgagcggtc cttcaaattg gctcgccaca cgcggccaat cttcctgttt atgtgcgtcc
    10141 cacaatttgt gcagggtctc tcgatccatt gagcgaggtt cctcatgcta gggccggcca
    10201 atccaaccta gcgtatgcag gagcagtacc gcaagggtgc agaggccaag gccccagaat
    10261 cgatagtcca cattttctcg ggtcagacac caatcgagga agtgttgacg caaggtcccg
    10321 gggcccagcc acaggaagag acccttcagc atcattccta tgcctatgat gatccaaaga
    10381 agtccatagg ggagatcgaa tccgagaatg agtaatgcga taccggcagc aacggcgagg
    10441 atctgccagc ggaatatttc cggcttgttc tgtacaattt cacgcacccg atccatcacg
    10501 acgcggggcg ctgtgagtaa tgccacccca tcggcaagcc agactcccgc gaggcaggcc
    10561 aacgcatatt ctttcatcgg cggaggtacc ggcttctacc ggagctgttg aggaaacatt
    10621 cctccggtca cggataaaga ccgcggtgca aatgagcctg ggcgacctta tcaatgccgc
    10681 tcatcagcgc cgccatgcgc attgaagtgc gtttggtggt ggcgaactcg atggttcgtt
    10741 ggaaggcaga cgtcatcaga tctcgcagcc ggtcttgaat gtccgcagct ttccaaaaga
    10801 atcgctgcgc gtcctgtacc cactcgaaat acgacacgat gacccctccg gagttggcca
    10861 gaatatcggg gatcacgaaa attcctttgt cctgcacgat gcgatcggct tccaatgtgg
    10921 tcggaccatt cgccccttcc gccaggattc ggcagcgtaa cttggacgcg tttcgttcag
    10981 tgatctgttc ggataaggca gccgggacga ggacggtgca atcaagctgc aggagctcat
    11041 cgttcgtgat atgatctccc agcttcgtgt ccttgaggga ccgacctgtt tctctgtagc
    11101 gggcgagaag tgtcggaacg tcgaatccac ttgaattata gatgcctccc tggacatcgc
    11161 tgaccgcaat cacgcgggca ccggattcgt gcatgatgcg cgcagtgtgt gaacccacgt
    11221 tcccgaagcc ttgaatggcg actgtcgtgt tccggatgtc caaattcaag cggcgaaggg
    11281 cttcgaatgt cacatatacc acgccacgac cggttgcctc ttcccttccc aggctgcccc
    11341 caattgaaag aggctttcct gtgacgacac caggcacgga gtacccaacc tgttggctgt
    11401 aggtgtccat gatccaggcc atcacctgag catcggttcc gacgtctgga gcggggacgt
    11461 cttgatccgg tccaataagg gggaagattt ctgatgcata acggcgtgtg agtcgctgca
    11521 attcagcccg ggacaattgc ttggagtcga ccgcaacgcc acccttggct cccccatagg
    11581 gtaggtcggc caaggcgcac ttccaggtca tccacatggc caaggcggct acttcaccca
    11641 gattgacgtc ggggtgatag cgaatgccgc ccttcgacgg accgcgagaa gaatcgtgct
    11701 ggacgcggta tccggtaaat acttcgaccc gaccgtcgtc catcctcaca ggaatactga
    11761 cgatgagtga tcgttgcggg aacttcagcc gttcgcggag attgggatca agattcatcg
    11821 cctcggcggc ctgatcaaac tgtgcgaccg ccaatcgaaa cgttgcggta ttgagttcgc
    11881 gcaaggaaga catggacagg ctaggcctcc ttcatggctg aagaataaag gcaacgatca
    11941 acaagctcgg ctcgccatac tatcaactgt aatatgggtc caggcgatat tgaacaccgt
    12001 tgagaacgtc tctgctattt gatcagcgat tcgctcaagc ggaatctccg attgaagaag
    12061 atttgcgaca gaggttgtcc ggctctgggc gagaccgcag ggggtgataa gcgaaaaagg
    12121 cgtgaggtcg aggttcacgt taagagcgaa cccgtgcagg gtgattcctt gttcaagccg
    12181 gacgccgatc gcggcgatct ttgcctcgcc ctgatcatcc cggacccaca cgcccgggct
    12241 cctgtcgact cgatgccccc ggattcccca gcaggtaagc gtacggatca acacttcctc
    12301 aagcagctgg acataggttt taggccctgt ggcgtaatgc gcaagctgaa gaatgggata
    12361 gccgataagc tgcccgggtc cgtggtaggt gattgaaccg cctcggttca ccgcataaac
    12421 cgaggcgccg gtctgtttga gggccgtttc gcctataccc atgtgagctg attctgttcg
    12481 gcgccccatt gtatagactg gctgatgctc taggagcaac agtgaatcct ttccagctcc
    12541 tgacaggagt tcggcatgga gttgctgctg gagtccccag ctttccatat aaggcaccgg
    12601 atgtcggaac ctcaccagtg ttcccggttg aggattctgg cgagcaaacg acatgatccc
    12661 ttctccgaga ctcgtgaaac cactaggcat gcagtcggat gaattcactg gggcgatttg
    12721 atcggatgag gtttcgggag ttggagagtc gcgggtccac tcttcccgtg gagcgtgagc
    12781 tggaccgtcg gctggtctcc taccctgagc accgcgtgcc aaaaatcccc gacattgaaa
    12841 atctgatgct ggtcaacagc cgtgataatg tcgccgttct gaagcccccc gactgcagcg
    12901 ggtttggcgg catcgacctg cagggccagc actccacgat caccctccag cccgaaactg
    12961 gccataatgc tcggcgtgat cgtgatgggg gtaaacccga ggtctggtcg gtatatcgaa
    13021 ccgcggacga tcatcgattg aatatgcggg tagatggctt ccattgatat cgcataactg
    13081 atggcttggg cggagggggc gatggcgaca ttgatgccga taacccgccc ggccaggtca
    13141 accaacggtc caccgctgtt gccggggttg atggctgcgt cagtctggat caaatcgtag
    13201 agtgtctcgc catcaggagt cagcaccgat cgatcgagtg cactgacaac tcctacggtc
    13261 actgttgagc cgcctttgag cgcgagaggg ttgccgatcg caacgacgga ctctccgatt
    13321 tccaagtcgg gagagggact gagtgtggcc gggaccaagt cctgggcatt gatcttcacg
    13381 agcgccagat ccaggagaaa gtcacgggct accacacggc ctggggtcag ccgaccggtt
    13441 gaaagcccca cgacaatgct cttgatgccg gtcacgaggt ggttgttggt cagaatgtaa
    13501 ccgctctcgt cgataatgac accagagcca gatcctgact gggcttgcga ggggctcgct
    13561 ggaacaccgc gagtgaggat cgtgacgacg gaggggcgaa ccttcttgac cacttcagga
    13621 acagacagcg gtttgtcgtg agaggtcgcg gtgccggtgc cggaatccgc ccaggccaac
    13681 gggataccca agaagcagag gaacgtgaga gtaaagaaag ttctggcagg ggagggcaca
    13741 gttgacgaag gtgtcatagg cctcatcttt cgaagcggcc tcattctcgc acaagtcttc
    13801 ccgcagggaa agccatggaa acaaagaaaa gggcgaggat ggctcctcgc cctttcgctg
    13861 gcacggtgtt tcacgattac tgaatagcgg cgaacatctc cttgaccgcc ggtgttttca
    13921 gcttcttcag ggccttggct tcaatctgcc gaatccgttc tcttgtgacc gacaagtttt
    13981 gtccgacctg ttcgagtgtg cagggctgat catacccaat accaaatcga agccggatta
    14041 ccgtttgctc tcgtggagtc aaggtcccga gaatccgctc gagttgatgg gccatttccg
    14101 tgcggtggac gtgggcatca ggcggcacag cctgcaggtc tggaagcagc tcgccgaatt
    14161 ctgttccgcc gtcaccgacc ggatgttcca atgccaccgg ttcctgaaat gcttgaaccg
    14221 tttcatgcag tcgctccggt ctcattctca ggacgttggc gatttcttcc agcctggcgg
    14281 gccgtccgag ctgctgtcct agccgcctcg tgacacgcag gatgcgatgc gacgcctctg
    14341 tctgatggac cggaatgcgg atcgtccggg actgatcggc caaggctcgc gtaatgccct
    14401 gcctgatcca ccaggtggca taggtgctga acttgaaacc cttgcggtat tggtaccgtt
    14461 ccgccgcttt catcaaaccg atattgcctt cctgtaccag atctagcagg gtcaggccac
    14521 ggcgggtgta atgctttgcg acatcaacga ccaaacgaag gttacagcgc accagctcgt
    14581 cttttgcctg ttcaagggta gttcggccct cgcgaatcgt tgtgagagcg ctcttcaatt
    14641 gtttgaccac attcgccggc agcttctgtg gaccggatcc gagtttaata agactgctaa
    14701 ggctttgttc agccgtgtcc agtgccgtgg ctgaaagtcc gctcagcctg cggaccgtct
    14761 ggaacgtatg ttgggcctcg atgactgcct cggagcgctt cgcacctgac agcagtcgat
    14821 tcgcatggcg caacgtcgtt cgaattatgc tcgaaccctg atcgatccgt ttggccaacg
    14881 cagtctcttc ctctcgcgtc aagagcgccc gctcaccaaa cgattggaag tagagcgatt
    14941 ctaataagaa cggaccatcg gccatgctct gacgcttaac gggcttgatt gcgtcctgca
    15001 attgcttctc ttccgtagag tctcgcccga tcacatccag caaatcgctg tcttcagaat
    15061 gatcggcatc ggcatccttc atctctggcg attgcttttc cggttcatcg gcagagaata
    15121 aatcgttttt catgtgaccg cccctcctct cgccgtcaag tttaggctcg ctgtctgcct
    15181 tcgattcgtc ggtaggcaga taggtcctat ttagtgagag gcaacccacg gtaaaaagat
    15241 tcaaccgtgg cgcctttgta cgtccattcg gcacaattat ctacgcagca agtcctatgc
    15301 tggatggatg atgagaatgc ggagctgcca tggtgctgaa aaagcgcgat tattaaaacg
    15361 ccctaatgag aagtggcggg gaagactgag gctattctgc ggcgattgtg tatgttcgga
    15421 gatatggtca aagccgcccc gccacatccg aggatggctc tggttggagt gcgcctagtt
    15481 cttacgactg aaccagaaca gccgtcagtc aacaaaaagg aagtgaagat gtgtcggcag
    15541 ccttgttgaa aacccggtgc ggcgcttcta gaatgggcct cccatgtccc actttcgaaa
    15601 cggcgaacgc atccacctgg tcgacaagaa agggcgtcag tacgccttga cgctcaaggc
    15661 tggcgatacc tatcagttga gcggtcacaa gattcctcat gacgaactga tcggtaaact
    15721 tgacggatcg cttgtgaccc tctccgggga aaagccaatg atcgcgctgc gtcccacgtt
    15781 cggcgaatat gtgctcaaga tgccgcgcgg agcgcaagtc ttgtatccca aagatctcgc
    15841 cctgattcct atgtgggcgg atgtctatcc tggcgcgcga gtctttgaag caggcaccgg
    15901 atctggtgcc ttaacgatgg cactcttgcg ggcagttggc ccgcggggtc tggtggtcac
    15961 ttatgaggcg cgtgaagact ttgcacgtat ggcgatgacg aacatcgaac ggtatatggg
    16021 gacgatgtcg aatctcgtcc ccttccgcaa gaacgcatat gaaggaattg acttgctcga
    16081 cgacggcctc ccctttgacc ggttggtcct cgatcttcct gagccatggc aagtggtgcc
    16141 tcatgtggcg ctggtgctgc gatctggagg aatgtacctg agctttgtgc caaccgttcc
    16201 tcaggtccaa cgaaccgtgg aggctttgga gcgcacaggg gtgttcggca tgatcgagac
    16261 gtttgaaaca ctgcttcgta cctggtcaat tcagggtcgc agcgtgcgac cggatcaccg
    16321 catggtcgcc cattcagggt ttctcacggt cgccaggaaa gtggagtcgg gattctggac
    16381 cgcgaggcag gcgagtagcc tggggtccaa cgacatgagt gatcaggaat ctgaagagga
    16441 caaggcatca tgaatcgact ggaggggaaa gttgccgtcg ttaccggcgg gaacgcaggc
    16501 atcggcgaag ccatcgctaa acggtttgcc gaggaaggcg cgtcggtggt cgtgaccggt
    16561 cgtcggcagc gggagttgga tcgtgttgtg agtgtcattc gacgtagcaa gggcgaagca
    16621 ctcgccgtgg ccggctcggt tgccgatgaa gcgcacgccc agaatgtcgc gcggcgggcg
    16681 ctcgacagct tcgggcgagt cgatatcctg gtgaacaacg ccggtatcgg ggattttgga
    16741 aagcgccttc atgagattga cgatgccacc tgggcgaacg tgcttgatgt aaatttaacc
    16801 ggtgtcttcc gcatgacgcg ggccatcatc cctcagatgc tcaaacaggg gcgtggcgcg
    16861 atcgtgaaca tttcgtccat tgccagtctc gtcgggattg caactctgtc agcctatgct
    16921 gcatccaaag gcggactcga tgcactcacg agagctctcg cgatcgacta cgccaaagac
    16981 gggatccgct gtaacgtggt gaatcctggc ttgattgata caccaatggc ggctccattg
    17041 atggcggatc cggaaaggct caagccgatc ctggcgcaat acgcaatcag ccgtcccggt
    17101 acaacagaag aggtagccaa tatggtgcta tatttggcat ccgacgaagc ggcttgggtg
    17161 acgggcggga ccttcacgat tgatgggggt atgacgattt acaaggggta gaggggagat
    17221 gacgatgcag aatacgatgg cccaagccag caaggccgca gtatggagag cggggctgac
    17281 tattgtctgc gcgatcctcc tgttggtgcc gggcctcttc ttggtgcagg ccggagaggg
    17341 cacggagttt cctggcgtgg tgctcatggt cgaccaagct gcaggaaagt ttgcggtcaa
    17401 gaaagacggg ggcgggaccc gctttacatt cgtggcgaac gagaagacga agttcagcgg
    17461 cagcgggcta tcctcgttga aagatctgaa gaaagacgat cacgtggtgg tgttctatca
    17521 cgtacagggt tcccagtacc tcgcaacgag cattgccaag aaatgacgcc tcctgcgatg
    17581 gatatcaacg ctcacactca attctgcgga gtcatcggca atccggtcga gcattcactt
    17641 tcgccggcca ttcacaatgc ggcatttcaa acgctcggtc tcaattttgt ctatctggct
    17701 tggcgagttg aagcgatagg cgacgcgatc aagggcctcc gcgccttggg aaatttccgc
    17761 ggagcgagcg tgacaattcc tcacaaagtt gcgaccgtgc cgttcctgga cgatttggag
    17821 tccacagcgc gtgacatcgg ggcgatcaat accatcgttt ccgatagcgg gaagttgaaa
    17881 gggtacaaca cggacgccac aggagcctta cgtgcgctgc gtgaaggcgg tgtgggtttg
    17941 aaagggcagc gggtcgtgat gttaggctct ggcggagcgg cgcgcgcgat tgccttcgcg
    18001 cttgcagcgg aaggagccat cgattgtttg gttctgctcg ggatcgataa gaaggaacgc
    18061 aatgcattgg ccggcgatct gcgatcaaag accgcgatag ccgttgatga taggcatctg
    18121 gacgaagatt cgctgaaaca agtcctgtca aacgcgcagg tgttgatcca ctgtactcca
    18181 accggcatgt ctcccaaggt ggaggagacg tgtgtgccct catcgcacct ccatgcggaa
    18241 ctcgctgtca tggacatcgt gtataaccct cgagaaaccc ggttgttaaa agatgccaaa
    18301 ggggccggct gtcggaccat tccggggttg gaaatgtttc ttcatcaagc tgccgcgcag
    18361 ttcgaactct ggacaaaccg acctgctccg accgaggtca tgcgcgcggt cctggaatcc
    18421 cgattctcat gaatatcgtg cttattgggt atcgcggcac ggggaaaagt acggttggaa
    18481 aactactcgc tgcccgcttg agccgtgaac tcgtctcgac tgataaggaa atcatcaagc
    18541 gggcggggcg atcgattccc gagatagtcg cggagcatgg ctgggaatat ttccgcgatc
    18601 tggaatcaga tgtctgccgt gatcttgccg gccgtgacaa tttggtcatc gataccggcg
    18661 ggggggcaat tcttcgccag cagaatgtcg atgtattaaa gagaaatggc gcgttggtct
    18721 ggctcactgc gtctgttgaa accattgcga gtcgaatcgg tggtgataca caacggcctt
    18781 cgttgactgg taagaagtcg tttgttgatg agattcagga cgtgttgcag gagcgaaccc
    18841 cgaagtatca ggaggctgcc gaccacattc ttcccaccga tggtcgttcc gtgaatcaat
    18901 tgctagagag tctgctcgga ttactcgggt ccgcagtgag tcggtcaatt cctctgtgag
    18961 gcgattgctt gacaagattg ctcttgaagt gtttgaagta gcttcgtgcg cccgtagctc
    19021 agttggatag agcgccgggc ttcgaacccg taggtcgcag gttcaattcc tgccgggcgc
    19081 accagcctgt cgttaaaata tcctgctcta tcaatggctt accagtttat ccgtttcgga
    19141 tctgttctcc gctttgattt aaatcccccc accaaagctg catagatttc tggagtttca
    19201 agttgtaacg ttagggtaga gggctagcag acgggaggcc gacggagtgg tatcctgtaa
    19261 tcaacagcct gttcttctga gatctcctga tgggagattc ggtagccacg aagtacagcg
    19321 ggcatggaca agacattgcc cctgctgtgg ccgttggttg acgaagcccg atcctatggc
    19381 cccgtgggtt tgttcctgcg gctggcagtg cggatgagcg cacaggctag gaggggagat
    19441 tataaatgaa tcacgtcatc ctccacgatg ttcgggccgg attctttctg atggtgggat
    19501 tcgccatgtt ggctcttata tgcgccgtcg cagcattaag tacggttatg cggcctgtac
    19561 gctgggcgga tcggaggtcg gcgcagtttt agcacgatgc gctcggttaa gcttcttaga
    19621 ggctaatgtg tagccgtagt ttcttgttac ccacccttct ttcttaacca gaactcactg
    19681 ataattcagg actctttcca tcaggcccgc acctgcctgg atctcgttaa tccggtgcta
    19741 tagttgatct agaagatttt tcttccaaca aaaggaatgg gggatatcat gaaggcgagt
    19801 ttaggaaaat ccggtctggt agcggctttg ggtgtaatcc tgttggcctc ccaaggttgc
    19861 agcatgaaat ggttgcaatc tgatggtgag gatgttgctg gtacagcaaa gagcggtagc
    19921 ggctctgagt ggaatggtca aggcgttaat ccaaattccc cgggaatggc ccaaagcggc
    19981 tcgcagtctg aactaagcgg tttctcgcag aatccttctg aagaatactt ggcctctgga
    20041 agcggaattg cctcgttgtc ctcgtcagac atgaatgctc ggcatcatgc agaattgacc
    20101 aaagaagaaa aggccgccat cgaagcgggc ctccaggacg tcttctttgg ctacgatcaa
    20161 tggaccttgt cggatgccgg aatggaagct ttgaatcgcg atgcgaagtg gctgaaagaa
    20221 catccgggtg ccgtcatgaa ggtcgaaggt cattgcgatg aacgcgggac aactgattat
    20281 aacgttgtgc ttggagacaa acggtccaag agcgcgcgca gctatctgat cgagttgggc
    20341 gtcagtccaa agcaagtagc aattgtctcg tacgggaaag agcgtccctt ctgtctggag
    20401 cacgatgaag cgtgctatca acagaatcga cgcgggcatg tgcttctcag cgtcaagaaa
    20461 aagtaacgaa cagcgacaat gcggtaatga ggccccggcg ctttcttaag aggtgccggg
    20521 gttcatcgtt tccagctgaa ttttgcctgc ccaggggatt cagcttgtgg tgctctaaat
    20581 tgttacggct ttgtcaggat cgctgagagg agtgttcgga tatgacggca agatcgcagg
    20641 cactcaagga agaacgttgt acggaaaaac gacaacaacc gagattcacg gcgcagttcc
    20701 gcagcacctt ttcaggcggg cagcgcgaag ggcgaggcaa gaccctggat ctctccgatg
    20761 ggggatgcag aatcgaaacg gatgagccga ttgttgtcgg cgccgcgttc gaatgccgaa
    20821 tctacgcgcc cggtctggat tggcctcttc gcatcgacga ggcgcaagtt cgctgggtca
    20881 agggcaagac ctttggcgtg cagtttgttt cgatccgacc ggatgaacgt gtgaaactga
    20941 aacgggtcat tgccaacctc cagtcagaat ccgagtaacg accctccttc ctcttgcggt
    21001 acagtgttct cctcctccca gccttcggct cgctgctgac tttctgccta tgctataaaa
    21061 gcgatcgcgc gattggaaga accgcaaggc cttgtcgacc tacttctatt cggatcactc
    21121 agccagatgg agggagcatg gtcatgacac aagaagttgc aacactcgct ggtggatgct
    21181 tttggtgtct cgaggcggtc tacgaccaac ttagaggtgt agtgtccgtc gagtccggtt
    21241 acattggcgg gcatgtcgat cgaccaacct atgaagcagt ctgtggaggc cggactggtc
    21301 atgcggaggc ggtccggatt acctttgatt cggctgtcgt gagttatcga gaactcctgg
    21361 aagtgttctt tgtggtccat gatccgacga cactgaatcg ccagggccac gatgttggga
    21421 ctcaatatcg atccgccatc ttttatcata atgccgatca gaaacaggtg gccgaggatg
    21481 ttatcgcaac gttcaagaag gaaaaggtct atccgaatcc tatcgtgacg gaggtggtgc
    21541 cggccggatc gtggtttgag gcggagccgt accatcagga atatttcgcc aggaatccct
    21601 tccagggcta ttgcacggcg gtcgtgggac cgaaagtgat gaagtttcgc aaactcttct
    21661 cggaaaagct caaaccctag ccgagtgctt tctaccgctt ccaccattcc atcaggcgac
    21721 tctgcgcgcc gggaaagaaa gctgttttct gcatgctgtg agggacgacg aacaggactt
    21781 ccgcttgcgg ggggcagaag ggccattcga tcgctgtctt gccctggacc ttcgcgagga
    21841 tcaatccgtc aggcctgtcg gatggcccct ggaggaccgt gagcggatca tggggatcga
    21901 ccagacatgt gccattgaaa ttggcgctct catacaagag gggatgggcc agggggacct
    21961 tgagcactcc gtctgagaaa attgtatcac cttctcgaat tggaccaggt ggtgcgatcg
    22021 aatagagcac tactggtaca actaacatga gggctacgac gaggccgatc gaccagaggg
    22081 gaggtccgga ttgttgaaga tcttcctgac gcggaggcat gacttcgttg ttcggtcaga
    22141 cggcgttgtc ttgatccagc caggcatctt cgtcgccttc tgccgcccct gcaacgcgca
    22201 aggatgaaaa atcaaatagc cgtttgtcca gcaacaatga aggggcgaca ttggagagcg
    22261 ctgcaaacat gctgtcggtg cagctgggcg ctttcctttc ccatgcccgc aagaactctt
    22321 ttacctgttt ccgtttgaga tcctcctgcg acccgcagag atcgcagggg atgattggaa
    22381 agtttctgcg ttccgcatag cgagccagat ctgcttcccg cacggcggcc agcggacgga
    22441 tgaccagatg ccgtccatct ttggatcgca gcttcgcagg catggtcttg agtttcccgg
    22501 tgaacaagag gttcaagagc aatgtttcga gaatgtcatc acgatgatgt ccgagcgcaa
    22561 tcttggtcgc gcccaattcg gtcgcaacgc gatagaggtg tccccggcgg agcctggagc
    22621 agagcgagca ggtggtctgc ccttcaggaa tgagccgttt cacgatcgag tacgtatcgc
    22681 gttcttcaat gtggtagggg attcccaccg atgcgagata ctcgggtagg atgtgagccg
    22741 ggaagccggg ctgtttctgg tccagattga ccgctaccag gtcgaagcgg atcggggcac
    22801 gccgctgcaa gacgaggagc aagtcaagca acccataact gtcctttccc ccggacagac
    22861 aaaccatcac cttgtcgccc tgttcgataa gaccatagtc gctgatcgtt tgaccgactt
    22921 ggcgtaacaa tcgggtctgt aactgttcgg tggcctcgtc caatctgggg ggagcaggga
    22981 gatggctgct tgtttgcatc atggccgata ttgtaaacgt tcgcctaacc aactgtcgat
    23041 cctccggaca gagggccatc atgagtcatc gatactggcc gcaatcggta ttgtttgtat
    23101 gcacgggaaa tattttcagg agtctggcgg cagaacaggc actcaaaacc atgctcggag
    23161 ccgaatcatc cctccatgta ggatcggccg ggatcgatgc caaaccgcag gctgtccatg
    23221 aatgggttca gaccaggctg cgcctcaagg gcgcagatcc ctcaggccat gtccagcgcc
    23281 aactgactat tgagatggtt caggcagcgg atctcgtgat tgcgatggga tgcaatcatc
    23341 agttgttcat tcgagatcgg ttcggacatg acgtgcctct gttcaatcaa gtatgctttg
    23401 gccgtgacga gcccattgag gatctgcacg aggtgatgcc caagtgggaa cagaatcttg
    23461 aagaggctcg cgcctatgtc tggtccgtga tcgatagtat ttgggaagcg acgccagcgc
    23521 tcatccaacg cctgccgtct ctccgttcat tgaaataacc ctttcattcg ttctccgacg
    23581 gttcattctt ggacagaaag gacaatttcc cccgatttta cccgtaaaga cctctccgcc
    23641 gtttcttccg ttaagcgagc tgaccgcgcc tgctatattt tctatgagat atctgtcttt
    23701 tggaaggcgc tatgtcgcga tcagggttac gggaagtgac attatctgac attggccgat
    23761 cggagccggc ctgggtttca tggtccgacg agaagctgtt ggatcttcag atatgtcgcc
    23821 tcaacgtcac tctcgacaat cccttcctgt ccggaaacat ccagcagctc tacgatgaac
    23881 tcgaggcgaa gcaactgcgg ttccgtccgc acttctggtt gtccaacgaa tggtttacgc
    23941 ccgacggtgt tcccggaatt gcgatcccgt tttatctcgc gcaccctcgt ctcgcaaaac
    24001 ttgagcttgc ccaaatgttg gaggtcgaag gagggacagc cgaatggtgc atgcgtattc
    24061 tgcggcatga agctgggcat gcgatcgaaa acgcctatcg catccggcgc ctccgaagcc
    24121 ggcagcaaat ctttggccgc tcctcccagc cctatcccaa atactacagc cccaggccgt
    24181 acagccgcag ctttgttcga catctcgatg tctggtatgc gcaaagccat ccggacgagg
    24241 atttcgcaga gacgtttgcc gtgtggctta cgccggactc gatgtgggcc gaacgttaca
    24301 aaggctggcc agttctcaga aagctgcaat atatggattc cctcatggga gggttcgtta
    24361 atgtacctcc gattgtcacg acgaaggaac taatcgaccc gctgcacact ctgaagaaaa
    24421 cgcttcggga acattacgaa cgcaagcgga gccactacgg tattgcaaga tcctcgttct
    24481 atgatccaga tcttaagcgg ttattctcgg atgcgccgac ccatgccgac agggtgagcg
    24541 cggcgacgtt cctcaaccgg ttccggcgtg aggttcgccg aaaggttgcc agttggacag
    24601 gggagtatca gtacacgatc gatcaggttc ttgaagacat gatccagcga tgccgagagt
    24661 tgcatttgcg tctgcccctt ccggaagaac aggcgaaact ggactttacg attttgttga
    24721 cagtccacac gatgaactat cttcgcagcg ggcggcacaa ggtggcagta tgaggcgatt
    24781 gcgcattctc gtcttgatgc ataaggattt ggttccgccg gaaccgctca acggtcatga
    24841 tccgttgcgg gctgaatgga aaacggaata cgacgtggtc acgaccttgc gcaagctagg
    24901 gcacaaagtt aaaccgctgg gcgtgaaaag tgatcttggc gtaatcaggt cggcggtgga
    24961 ggagtggaaa ccgcacattg ccttcaacct gctcgaagag ttcgacggcg ttgccgtcta
    25021 tgatcaaaac gtcgtctcct atctcgagct gctgcacatg ccctataccg gctgcaatcc
    25081 ccgcggcttg atgctggcgc gcgacaaggt gttatcaaag aaactcttct cgttccaccg
    25141 gattccgttc ccggaattca tggttgtgcc ggaggggcgg accgtgaagc gacccaagtg
    25201 gctctccttc cccttgatcg tgaagtccgt caccgaagag gcatcgctcg gcatttcgca
    25261 ggcctcgatc gtccaagacg atgagaagtt gaaagagcgt gtcgcgttta tccatcaaag
    25321 agtcgggact ggggccctca tcgaacggta cattgaaggg cgtgaactct acgtgggcgt
    25381 gttgggcaat gggcatctgc aggtattccc ggtctgggaa ctcaagatgg acaagctgcc
    25441 ggaggatgcc cgtcggatcg caaccgagcg ggtgaaatgg agtcggaagt atcaggataa
    25501 gtatggaatt acctcgaaag aagcaaagaa tcttcccgaa ggaaaggccg aggagatcca
    25561 gcatctcgcg aagcgggtct atcgcgcgct cgggctgagc ggctatgcac gcattgacat
    25621 gcggctggat gagaagggac agctgtacgt gctcgaagcc aatccgaatc cgcaaatcgc
    25681 gcacagtgaa gattttgcgg attccgccga gaaggcgaac tacccctaca aggagctgct
    25741 ccaagagctc ctaaatgttg gtctccgctg gcggccagcg aaagccgcat aagccgactg
    25801 cccaagatcc cagttcagct cttcatgctt cagagaaaca gtgagcgcag aatctcgagg
    25861 gcgttcttgc tcagtgcaag ggcgcgcttg aagtgctccg ctgcgccgcc tgtccatcat
    25921 ggccggatgc cagcacgggg agtgcgaggg acaagttgct tgagcgctcg gccgcgctgc
    25981 ggtattctcg caaatcggtc agcgagacgg ggtgtggatg ccaggaaaag gaatgacacc
    26041 gagagaagcg gcatcggcgg tgatcgccgc gatgccgact ggtctctcgg attcccagtt
    26101 gcaagagtac gggatcgaag cgacggccga acgggcgcaa gccctcaccc gcgaaatgct
    26161 ctcgctcaat ctcttttgga tctttgcggc catcgaagcc catatcccgc agaagttcca
    26221 gcaggcggta tcggcgctcg tcctgcacgc agtcgaaacg ggatggggca cgacctatcc
    26281 cgttggtccc gtagcctgga cgacctttct gacggaatgg aaagagcgga cgaaacgcta
    26341 cgagcgcttg gtgaaagatg ggatgagtcc gcttgcgatc agcgccgaag tcggcatgtg
    26401 gctggaagaa caacacatcg tcggcgaaga ggaccggcga tatgttctga ccctgctgat
    26461 cgactcagtg ccggtcgaga gctacgggca gctactgcaa gacgtcggct gagagttatg
    26521 ccacttacag gtatcgatgg tttatgacct tgccggtggt atcgagttca tatagcagcg
    26581 gggcgcccgt cggaatattc agttcgagca cctgttcttt ggagagctgc tcgaactgca
    26641 ttaccagcgc ccgcagactg ttcccgtgtg ccgcgatcag aattgtatcg cccttcaaga
    26701 ggtggggttt aattttctgt tcgtagtacg gtagcacgcg atccgccgtg tctttcaggc
    26761 tctctccacc gggcgggggg acgtcataac tccgccgcca aatcttcacc tggtcgtcgc
    26821 cgtacttctt tgccgtctcg gccttgttga ggccctgcag ctccccgtac atccgttcgt
    26881 taagcgcctt gtctttctcg atgggaatgc ccgactggcc gatcgtttcc aagacgagcc
    26941 gcagcgtttc gatcgctctg gtaaggacgg aggtgaatgc gcgattgaag gtgaagcccc
    27001 ggagcttctc gcccgcgttc cgcgcctcct ggatcccacg tggtgacagg gggacatcga
    27061 cccagccggt aaaacgattt tcgaggttcc attgcgattc gccgtgacgc agtaggatta
    27121 aacgacccat tccttcttcc tcccagtcgg agatcggagc ggttgcaggc ccaagaatac
    27181 tggatcgata ctgcaagtca agcgttgctt tcgtccttat gcggaagtac catgccgcac
    27241 aacttgagga ggttgcaaaa gcccgccagc agcattcacg caacgctcaa ggcctcaatg
    27301 tgcggtattg agcccgctaa tcttccgctt gctgctgcct tgccggcgga tattattacc
    27361 agcctccagg gttctatgag gcctcctgtg gcctggccaa gggatttcgt cgacgcttga
    27421 tcttctgaga gttttgcgac gatctcaagt gatctcaaga gcgaatcgct tgactgtatg
    27481 accatcccgc catcatccga gatcaattgg ccggccctcg aatcctggtg gatcgcgatg
    27541 ggcgatcgcc tgcacgtggc cgaccgcgcc cgatcttttt ttataaaagt gagaagcgac
    27601 gggcagctcg atcggatcgt gcaggtcggt ggacaggtcg attacatcct gttcgtgctc
    27661 atgcgctatt gggtcccgac cgtcctgccc ccgccaggcc gcgagcgact gacgaagaac
    27721 gatccggatt actggctgga atcggagcac attctcaaag ccgctgcagc ccggctgcgc
    27781 gaactcaagc cgttaatcga attgctcacg actcccggcc ccctttccga cgctccttcc
    27841 ggcaagggcg tcagcccttc gccggtcgcg gaactcgaac tctccagcat cgtcgaggga
    27901 attggagaag ctgccggcac ttacggcgga ccggactaca cctcgatcgt gaaagccttc
    27961 gacccggttc cgctccgtca aacgcagccg ttcaagcaca ataagaaaaa tagtgcggag
    28021 ctctgggttg tgtttctctt ccgcgaacat tttcggagcc tgggattggg gaaagatcgc
    28081 tactggccgc tggttgcgcc cttatgtacg gcagcgggta tcctcaatca gaacgggcag
    28141 gcattcagcc ccgatgaact caagtcctgg tggcagaaaa actggccgcg cacctatacc
    28201 cagttggaac agaagcagga cgctcccgac tcaggcggcg cggcctatgc acaggacttt
    28261 ggctggtttc aggcttggat cggctggcag cgtgctcggt aggctattgt ctacgtgtga
    28321 gttgtcgcgg ctggtttggc tggctgtgtc ggtagtcgct ctccgtccag cgcaccgtcc
    28381 tgaatcatct tgaacatcgt gattgaaaag agcacataga gggcgacgcc cacccaaatc
    28441 acgtagggtt ggactccgcc ggcttctttg accgcgacat cctgtttagc cggttcactc
    28501 cacaattgtt gtttgatcgt gccgatctgc tcccggcagt gccaatagta gacgtagttg
    28561 gctgttgcgc cggctacgat gctccagacg acaccgctcg tcatgtcgcc ggtcagatag
    28621 gtggaagcca tagggccgat cgcatagacg gccgcataga gatacatctt gcgatagaga
    28681 aaccagagga acgaaacgaa cagaaaggcc ggccaattcc atgtcagggc gaattttggc
    28741 gtctgctccg ggccgaattt cttgaattgt tccagatagc ggtctgcgtt gggaccgatg
    28801 aaggctcgcc agagatcgct gtcgctctgc gtgggatgta cggttgaagc aggcgtggcc
    28861 tgttccgccg gtgccgtcag atgagtcccg cattgatagc agaatttcga gtcgtcttgg
    28921 ttttgttgct gacactgcgc acaggatttc attgttcaga gagtatcaca tcgtcggggc
    28981 tgaacgggca aagaaggaat cgagaccttc ccttggcatt cgctgaccgg tagcaagttg
    29041 ctttcacagt agtgcctgtg ctagcttccc ttactttaca aggggatacg tctgccatgc
    29101 cgaccgaaga gctcaagcaa ggcaccgaac agtacttgat gggaacctat gcccgtcagc
    29161 cgatctcgat tgtgcgagga cgtgggacga aagtctacga tctcgaaggg cgggagtata
    29221 tcgattttgt cggcggggtt gccgtcaata ttctcggtca cggccatccg gacctcgtct
    29281 tagccattca acgacaggcc gcgcaactga tccacacgtc gaacctctac tacaccgagc
    29341 cgcaagtgaa gctcgcgcaa atgcttgtgg atcactcgtt tgccgacaag gtgttcttct
    29401 gcaacagcgg cgcggaggct aacgaagcgg ccattaagct cgcgcgtcgc tactcgcacg
    29461 acaagtacgg ccccgggcga ttcgagatca tcacgatgaa aaactcgttc catggccgca
    29521 cgatggcgac attgaccgcc accggccagg aaaaggtgca gaagggctac gaccctctgc
    29581 tgccgggatt tctgtacgtt cccttcaacc atctcgccga gcttgaccag acagtgagcg
    29641 ggaaaacggc tgcgatcatg ctggagccga ttcaaggtga aggaggcgtg catgttgcgg
    29701 atcgtgaata tttgaaagcg gtgcgcgagc tctgtaccca aaaagacgtc ttgttgatct
    29761 tcgatgaagt gcagaccgga atggggcgaa cgggcacact cttcgcgtac gagcagttgg
    29821 gtgtgcaacc tgacattatg actttggcca aggggcttgg cggcggcgtg cctatcgggg
    29881 cttgccttgc gaaggatgag atcgcgaaag cgtttggacc aggctcccat gcttcaacgt
    29941 tcggcggcaa tccgcttgcc tgcgccgctg cattggccgt ctttcgtgtc ctccttgaag
    30001 gacgcgtgct cgaccatgcc cgtcgcatgg gtgattattt ggcaaagggt ctctcggagt
    30061 ttaaggaccg tcatcatgca gtgaaggacg tgcgggggct cggcctcctg caaggccttg
    30121 aagtagagat gaacaccaag acggtcgtca gcgactgtgt cagacgcggc atcctggtca
    30181 acgccacggg agagcatgtg ctccgctttg tcccgccctt gatcatttcc cagccggaga
    30241 tcgatcgact catcgaagtg ctgtcgcaga tcttcaatcg gcaggcggcc gaagggccag
    30301 cgcattaatc gataggctct aatgccatga aaccatcggc gcagcgaacc actgggctga
    30361 gcaaggatct gctggatgtc gcagctctct ccaaagcgga ggtcgagagc ctgctgacgc
    30421 tggcaactcg cctgaaggat aagcagcggc gaggcgttcc ccatcccgta ctccagggca
    30481 agaccttggg gctcctgttt gagaagccct cgaccaggac gagagtctcc ttcgaagccg
    30541 gcatgaacca gctaggcggt cacgcactga tcctgccgat gggcgacatt cagctgtcgc
    30601 gcggtgagag cgtgcccgac acggctcgcg tgttgtcgcg ctatctcgat ggcatcgtca
    30661 ttcgcacgta tgaccatgca acggtccagg agtgggcgag ggaagcgacc atgtccgtca
    30721 tcaacggact caccgatctg agtcatcctt gccaagctct gtctgatttg ctcacgatca
    30781 aggaaaagaa agggcgcttg aagggtatca aggttgccta tatcggcgac ggaaacaacg
    30841 tggccaattc attgatcgaa gcggcagcga aaatgggcat gacaatctca ctgggctgtc
    30901 cggccggcta tcagccggat cagcatgtgg tcgacctggc tcggctcgaa gcggagaaaa
    30961 ctggagcgct gattgagctc agccacgatc cctctatcgc cgccaaggaa gccgacgtga
    31021 tctataccga tgtctggatc agtatgggcc gtgaacgtga gcaggctcgg aggctgaaag
    31081 tcttggcgcc ctatcaggtg aacagccgcc tcgtcagccg tgcgaaatcc gacgcgatca
    31141 tcatgcattg cttgcccgcg catcggggtg aggagatttc ggcggaagta ttggacggcc
    31201 cgcagtcggt cattctcgac caggccgaga accggcttca catgcagaag gctattttga
    31261 cgaaactctt aggcaagggg aaagaaggaa aacgatgagt cgtgcgatca agaaagtcgt
    31321 gctggcctat tccggaggac tcgacacctc cgtcatcttg aaatggttgc aggaaaccta
    31381 tcaagccgag gtcattgcct tttgcgccga tctcggccag ggcgaagatc tcaaagcgat
    31441 caaggcgaag gcgcaggcgc tcggcgtcaa gaaggtctac gtcgaagatc tgcgggaaac
    31501 cttcgtcaaa gactacgtgt tcccgatgtt gcgcgggaac gcgatgtacg aaggctgcta
    31561 tttgctgggc acatcgatcg cacgtccgtt gatcgcccgt cgtcaggctg aaatcgcgct
    31621 caaggaaggt gccgaggcgg tgtcgcacgg tgcgaccggg aagggaaacg accaggtccg
    31681 cttcgagttg acctatacgg cgctggcacc ggggttgaaa attatcgcgc cctggcgtga
    31741 gtggacgatg cggtcccggc gcgagctgat cgagtatgcc gatcgccacg gtattcccgt
    31801 gaccgcgacc aaagcgaagc cttacagcat ggatctgaac ttattccatg tgagttacga
    31861 agggggcatt cttgaagacc cctgggaggc gccgccggat gaaatttttc aaatgacggt
    31921 ttcgcctgag aaggctccga ataaaccgct ggaagtcgaa atcgactacg aggccggcaa
    31981 cccggtcgcg gttgacggca agaagatgag tcccgcgaat ttgctagccc acctcaataa
    32041 gttgggcggg gcccacggga tcgggcgtgt cgatctggtc gagaaccgct acgtcggcat
    32101 gaagtcccgc ggcgtgtatg aaacgccggg cggcacaatt ctccatgtgg cgcaccgggg
    32161 attggagtcg ttgacgatag accgcgaggt gcttcatttc agggatagtc tgatcccgcg
    32221 ttttgccgat ctcatttaca acggctattg gttcagcccg gaacgcgaga tggttcagac
    32281 ggcgatcgat gaggcacaga aggatgtgac ggggaccgcg agagtcaagc tctacaaggg
    32341 cagttgcacg ctcgccggac gcaaatcaaa gaactccctc tatcggctcg acatcgccac
    32401 gttcgaagag gacgaggtct ataaccagaa agatgccgag gggttcatcc gtctcaatgc
    32461 cttgcggctg aagatcagag cgcaacggaa gaaggcatcg tcctgatgag gagagcgaag
    32521 aagcgcagcc gggctgagtc cgcgagcggc aaggcgtggg agggacggtt tcgggaaaaa
    32581 acgaaccggc tggtcgaagc gttcacggtg tcggtggatg tcgatcggcg gctctatgcc
    32641 catgacattc agggcagcat cgctcattgc aagacactcg cgagagcgcg cgtcttgacc
    32701 ccgggcgaaa cgagggccat tgtgcgaggc cttgagtcgg tgaagacgga gttcgatcgc
    32761 gggcgatttc ggtttttgcc tcaggacgag gatattcata tggcgatcga gcgccgtctg
    32821 acggagttga tcggtccctt aggtggcaag gtgcacacgg gacgcagtcg gaacgatcag
    32881 gtggcgctcg atgtcaggct ctatcttcgt gaccagctgg atctcttgct ggcgcggctc
    32941 gaggagtttc agctggtctt ggtggccaaa gccaaggcga accggactct ggctatgcct
    33001 ggttataccc atttgcagcg ggcccagccg gtgctgttcg cgcatcatct gctggcgtat
    33061 gttgaaatgc tcgagcgtga taaggggcgc ctgcgcgatg cgaagcatcg gctgaacgtc
    33121 atgccgctag ggtccggcgc gttagccggt accaattatc cggtggatcg ccggtacaca
    33181 gcgaggctgt tggatttccc ggctgtcacg gccaatagtc tggatgccgt gtccgatcgg
    33241 gacttcatga tcgaaatcgc cgctgcgctt tcgattgcca tgatgcatct atcccggttg
    33301 agcgaagaac ttatcgtctg gtcttcgcag gaattccgct tcgtggacct cccggacggc
    33361 ttctgcacgg gcagcagcat gatgccgcag aagaagaatc cggatgttcc cgaattggtc
    33421 cgcggcaaga caggccgggt ctacggccat ttgatcaatc tgttgaccat gctgaaggcc
    33481 ctgccgctca gttataatcg ggacttacaa gaggataagc cggccctgtt cgac
//
LOCUS       DATIFR010000225        25971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1113337, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000225 DATIFR010000000
VERSION     DATIFR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25971
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113337"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2604
                     /gene="mutS"
                     /locus_tag="VLD60_12885"
     CDS             <1..2604
                     /gene="mutS"
                     /locus_tag="VLD60_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HSB45902.1"
                     /translation="RGYPEAILLFRVGDFYEMFYEDAQAASKLLSIALTSRDKSSTDP
                     VPLCGVPYHAAQGYIAKLLKAGRTVALCEQVEDPKLAKGLVRREVVRLYTPGTLVDTE
                     FLSPGESNFLAAVSRSERATAHPQPLWGLAGLDVSTGEFWVTEFHGPEATQQLLDELA
                     RLEPRELLHAGPAIADELGLRHLQGPRLCEQSPALFDHQEAEKLLRSHFTVHSLDGFG
                     CRDLTAGLGAAGAVWRYFRETQPTAALDHIRRLQRRWSHDAMHLDSATIRNLELVRPM
                     GVDEDRTGRRQPTLLSVLDRTTTAMGGRLLRDWLVRPLVDRSAIQARLEAVAELKERV
                     AQRVMIRTTLRAVQDIARLSSRITLGVAGPRELLALKQSLSALPELRAQLESVQSALL
                     ADQKNSWDDCRNLYDVIEQTLDPDAPMSARDGGLIRSGYHPGVDELRKARTEGKSWIA
                     ALEAKERERTGIDSLKVRYNQVFGYYLEVTKANLSKVPADYMRKQTLVNAERFMTPQL
                     KELEERVTGAETKLVALEEDLFVQLRERIAKDVPRLQTMAHTLALLDVLACLAETAAL
                     NRYVQPTLDESGMLLIREGRHPVVEQLSPDLTFVPNDTLLDGEANRLVILTGPNMAGK
                     STYLRQVALIVLLAQIGSFVPATEAHVGLVDRIFTRVGASDNLAAGQSTFMVEMTESA
                     HILNCATSRSLILLDEIGRGTSTYDGLSIAWAIAEYIHDRQHLGARTLFATHYHEMTQ
                     LESLREGIKNYRVAVQERDGDVVFLRKIVPGGADRSYGIHVAKLAGLPPSVIDRAQEV
                     LAQLEQTDSAAATPAPSSAQDPGSRLLPQSHPILDEVRQIDLFSMTPLDALNRLAELQ
                     KRAESEGNQQT"
     gene            complement(2660..3310)
                     /locus_tag="VLD60_12890"
     CDS             complement(2660..3310)
                     /locus_tag="VLD60_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45903.1"
                     /translation="MKFQSKGWAIVAAVALVVSVASTALAIESFQERFEWGDMSKPTT
                     LQGRVIVLDHYDEAVWINIAVFGGNAESGLFWQKIHPGKTLKFYADKGAWEELKKMGR
                     PHAGPAAAKEVPPGSTTDLIEFTATETEQNHRVISSVKKLPEVAGAPGKTRSISALRL
                     KECAGKDESDAGCAAAKQALNVAGKAQPPMQYDVLQPGGKPLAGLIPWTAQYNTGH"
     gene            complement(3362..3871)
                     /locus_tag="VLD60_12895"
     CDS             complement(3362..3871)
                     /locus_tag="VLD60_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45904.1"
                     /translation="MKRVVLLAAVAVVAVFGLLTAEFAVAADAPGAAGPADQIVGGKP
                     FKGEVTKTLKGRVWSQWADNPDHELLFGIQYWNTGEPASGTPSGRSSSDLDVKPPDPL
                     FSCCAWGFVGGTDRNNPYSGWYHAATTVRLAVKDKALMDQIIKASQELVAMEVSLDGR
                     TITGFKVIK"
     gene            complement(4222..5475)
                     /locus_tag="VLD60_12900"
     CDS             complement(4222..5475)
                     /locus_tag="VLD60_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB45905.1"
                     /translation="MYRKLVLDNGVRVVTERIPTLKSVTVGIWVNTGSRDEQPSQAGY
                     SHFIEHMFFKGTRSRSATEISREIDALGGEMNAFTTRETTTFYVKVLDQQLEQALNLL
                     ADLFHHSRFDPKEVEKEKQVVLEEIRMVQDDPEDLVQELHTGQVLGRHPLGRSILGRE
                     DTIRGLRRRDLMGYIDAHYDPSQIVIAIAGNFDQAALTKMVARSFGKTERRDGSVRAA
                     RRPPEVRGGLVTKKKPLEQVHLCLGLKGVAAGHRDRYAVYAMNSVLGGSMSSRLFQEV
                     REKRGLVYSIYSFLSGYSDVGTMTVYAATRPKEVERVVDLVCREIRRISRQGIEQREL
                     DRAKNQMKGSLMLSLESSHSRMSKLAKDELVYGTRTSLEDMLAQIDRVTTEQVYTVGR
                     QLLDLDHLAVTGLGPLTSRTLHAFR"
     gene            complement(5481..7601)
                     /gene="pnp"
                     /locus_tag="VLD60_12905"
     CDS             complement(5481..7601)
                     /gene="pnp"
                     /locus_tag="VLD60_12905"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSB45906.1"
                     /translation="MVHTVELDIAGRTLRLETGRVAKQADGSIWASYGDTVVLATAVA
                     SQNAKPGIDFLPLTVDYQEKAYAAGKIPGGYFKREGRPSEKEVLTSRLIDRPLRPLFP
                     EGYYFETQVIAAVLSSDKTGSSDVISITAASAALAVSNIPFLGPVAGIKIGRVSGQFV
                     VNPDLETLKSSELDLVVAGTADAVMMVEAGANELPEATMLSAIELAHAEIKKIVAKIN
                     ELRLAAGNKPKRAVVTEHIDPALTGHVKALVAQGIRDAIMIPNKTARQERLDLILADA
                     IEKLKNPEDPNRERHVKIVFHGLEYTEVRNMILERGTRADGRGPADIRPITCEVGVLP
                     RTHGSALFTRGETQSLAVITLGTTDDEQRIDALEGEYTRTFMLHYNFPPFSVGEARPL
                     RSPGRREVGHGALAERALKPVIPGKDVFPYTLRIVSDILESNGSSSMATVCGGTLGMM
                     DAGVPIKEPVAGIAMGLIKEEDRVMILSDILGLEDHLGDMDFKVCGTKQGVTALQMDI
                     KIGGITPALMHQALEQAKAGRLHILSCMQKALEAPRTTMSAFAPRIFTMKVKQDKIRE
                     VIGPGGKTIRGIQADCGVKINIDDTGTVTIASVDGASLEKAKGMINQIVEEVEVGKTY
                     LGTVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVSDEVKEGDQILVKVLEVDRQG
                     KIRLSRKETMPAPTGAGTKDQSSG"
     gene            complement(7750..8019)
                     /gene="rpsO"
                     /locus_tag="VLD60_12910"
     CDS             complement(7750..8019)
                     /gene="rpsO"
                     /locus_tag="VLD60_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSB45907.1"
                     /translation="MALLKEAKAELIKQYRQHDSDSGSPEVQIAVLTNRIAYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRDIDDARYRAVIDRLGIRK"
     gene            complement(8064..9017)
                     /gene="truB"
                     /locus_tag="VLD60_12915"
     CDS             complement(8064..9017)
                     /gene="truB"
                     /locus_tag="VLD60_12915"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSB45908.1"
                     /translation="MNGTIQGRGVMDGVLVIKKEAGWTSHDVVAKIRHLLGGIKVGHA
                     GTLDPAATGVLPVLIGKGTRIAEYLMEWDKEYLAILRLGETTDTQDATGTVLVRCATD
                     GVTSAAIEEAVGQFRGRIEQVPPMYSAVKVAGVPLYKSARAGKTIAREARQIVIHSID
                     ILAIDGRDVTLRVACSKGTYIRTLCADIGEALGVGGHLLALERRRVGPLTFDQAVSVD
                     EVEAHLALGRLGEDVQSLDQALQGLPAVVVDAQTADRVRHGVPVPASKVLRWESAGNV
                     DQKPGARVRIHDISGILLAIGRQPQAPQGSIAIEKVLVGQE"
     gene            complement(9038..9481)
                     /gene="rbfA"
                     /locus_tag="VLD60_12920"
     CDS             complement(9038..9481)
                     /gene="rbfA"
                     /locus_tag="VLD60_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSB45909.1"
                     /translation="MTPKGLRARAGQGERTMAKTGYSRADRVADQIRMEVADILMRKI
                     KDPRVRSVTVTDVELTNDLRIARVFVTALGTDAETKDVFAGLAKASGFVRSELGRRLT
                     LRYLPEVVFVKDISGPRGDRVLQLLDEIHVKTDAEGDAPQDPVSS"
     gene            complement(9478..9765)
                     /locus_tag="VLD60_12925"
     CDS             complement(9478..9765)
                     /locus_tag="VLD60_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HSB45910.1"
                     /translation="MSIIVGLCTIELFLPDSQSLKDKRQVLLSVKDRLHQKFNLSVAE
                     VDGQDLWQKAVLGLACVANDGRYVNQVLDQALNMIRSDPAVEIVQSRVELL"
     gene            complement(9785..12337)
                     /gene="infB"
                     /locus_tag="VLD60_12930"
     CDS             complement(9785..12337)
                     /gene="infB"
                     /locus_tag="VLD60_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSB45911.1"
                     /translation="MRVYELAKQLGMENRDLIPELKRLGIPVASHSSALEDDAVQKVL
                     EKLGPKHKTAAKIVTKAGEGDTARTEKHKDTDAGVPHHAGVKTAGAHPVVAEESKPDK
                     RRILIKRKRSEEAPLEESLAPLGSESLAAAATEAVAAPSVTPPSHHVESHMAPSGEVH
                     PPSGLIPSPAAPTLEQPPAAKPAVAPTIEPLSGKKKVFEELQAEGLKDKLKKARKPGR
                     VREEDDVRVREDAARWQDLRAIPVQRRDDRAKHVHHSTPAEITKPRKKSVKLTPGMTV
                     KEFAELIGQRPADIVRKLMDMGQMLTFNQPMNVDVASMIAEESGIKMDVAVEKGGEDL
                     LETVIQTEGTARPEPRPPVVTIMGHVDHGKTSLLDAIRETKVAEGEAGGITQHIGAYT
                     VSVRGKQVTFLDTPGHEAFTAMRARGAKVTDIVILVVAADDGVMPQTIEAIHHAKAAG
                     VPLIVAINKIDKPGANPDRVKNALSEHGLISEAWGGDTIMVEVSAKQKTGLDQLLEMI
                     LLQAEVLELKADPHQLAKGAVVEAKLERGRGPVATVLVQSGTLHVGDVFVVGSFSGKV
                     RALISHTGTKVQEAGPSIPVEVNGLPGVPSAGDVFQVVKDERVAREIAEDRARKQRAA
                     ELAGAGKVSLDDLFAKIQEGSVKELSLVIKADVQGSSEALAAAIEKLPTDMVKLKVIH
                     NGVGGITESDVLLAAASNAIIIGFNIRPEPKASALAEKERVDIRLYTIIYDALNDIKA
                     AMEGLLEPTLKERVLGRAEVRQVFTVSKAGTIAGSYVIDGAITRASAGVRVIRDNVVV
                     YQGKLGSLRRFKDDVREVQQGYECGIGVENFNDVKTGDIIEAYAIDKIAAKL"
     gene            complement(12372..13523)
                     /gene="nusA"
                     /locus_tag="VLD60_12935"
     CDS             complement(12372..13523)
                     /gene="nusA"
                     /locus_tag="VLD60_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSB45912.1"
                     /translation="MNRELISVIDEIGRQKGIDKSKVIGAIEAALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVDSVTNPKAEISLKEARAYDSEAEIGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNYLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKAMLLEVRRTPKDVQVILTRSHPQFVAKLFELEVPEVMEK
                     IVEIKAVVREPGDRTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     SDPRVFIAEALNPATIEKVGVDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDKEIKAALAEEAEAQRQQEEARQQEKAQAESEG"
     gene            complement(13555..14031)
                     /gene="rimP"
                     /locus_tag="VLD60_12940"
     CDS             complement(13555..14031)
                     /gene="rimP"
                     /locus_tag="VLD60_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HSB45913.1"
                     /translation="MKESRPVVDRISEAVSPILWTLGLELVDVVCVGQGPRSVVRVFI
                     DKPGGVTLDDCERAHKALGPALDVADPFPHAYTLEVSSPGLDRPFKRIQDYRRAVGKR
                     VSVKLREPLEGQWRLVGTLTEVTEHAVALVVSDPPPVRTLTLEYGAIAEARLKVTW"
     gene            complement(14154..14993)
                     /gene="dat"
                     /locus_tag="VLD60_12945"
     CDS             complement(14154..14993)
                     /gene="dat"
                     /locus_tag="VLD60_12945"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HSB45914.1"
                     /translation="MPDIALVNGRFMPLADAMISIEDRGFLFGDGVYEVIRTYRSRPF
                     ELDAHLTRLNRSARGLDLPQPYSHQQWHQLILEGIKQAAFPEAKVYLQITRGVAPRDH
                     AYAADLVPTVVMTVRELRPLSQSVQAAGVDAMTIEDIRWGRCDIKSINLLANVLARQQ
                     AKQAQMFEAILVKGEEVTEGAVSNVMVVRDGMVITAPEGPCILSGVTRAVVLQLARQE
                     GFPVQERYVARQELYDAAEVFLTGTTVEVLGVVRIDGKAIGEGKPGPLTQRLARRFVE
                     RIG"
     gene            complement(14996..15340)
                     /locus_tag="VLD60_12950"
     CDS             complement(14996..15340)
                     /locus_tag="VLD60_12950"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HSB45915.1"
                     /translation="MSMTDGGARPQDHAKMIEATLDGPIEIHLWEDRTRGELWVPTYD
                     TTGLALLADDYLRIAGNNAVDNGRRTFEFKAVKPGSYQIVFEKRMGWKFTAEDRRVFQ
                     VTVSEPSSSRSK"
     gene            complement(15413..15736)
                     /locus_tag="VLD60_12955"
     CDS             complement(15413..15736)
                     /locus_tag="VLD60_12955"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB45916.1"
                     /translation="MASEGFQLVAKVGEVPPGQSKVVTVNNRAIALFNIEGKYFAIHN
                     LCPHEGGPLNEGRVKGFVVSCPWHDLAFDIRNGQGTDGGGYCIGSYEVRVEGEEIFVG
                     PRRKT"
     gene            complement(15776..16270)
                     /locus_tag="VLD60_12960"
     CDS             complement(15776..16270)
                     /locus_tag="VLD60_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSB45917.1"
                     /translation="MASIPTSEREQKKKRIIMMILLAILLMSVSMFVMQREDSKTIIV
                     TFPNGTDLEAEVADTPEKLLFGLAFQEALPPNGGMIYIFEATGPHRVTTKEYRIPVDM
                     IWVDESHHIVSITENVPPCRQETCPWHGSTTEVVRYLIQTEAGFAKRQGLVVGGELKY
                     TLRM"
     gene            16487..16834
                     /locus_tag="VLD60_12965"
     CDS             16487..16834
                     /locus_tag="VLD60_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB45918.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAESKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCISDPAYPETTDVLLEKAKTLNPDK
                     TIDAAKVWSGVRN"
     gene            17044..17376
                     /locus_tag="VLD60_12970"
     CDS             17044..17376
                     /locus_tag="VLD60_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45919.1"
                     /translation="MRYRGILIGSFLVLLILAPLAQASTGDIGSIIEKFVDRQFPHAA
                     AHYWVINETQWDGDEMVVDVHTIVKERRDTEPILNRFLLLIVAGEVKGVQNVPLEPGT
                     DCRAEEDV"
     gene            complement(17467..17946)
                     /locus_tag="VLD60_12975"
     CDS             complement(17467..17946)
                     /locus_tag="VLD60_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45920.1"
                     /translation="MGKTMLGVFFGLVMVVAGASAAFALQAGGVEIGDLETGSVVGQP
                     FKELEVDAQLFAIEIGNMKTWYPPVTIIDFKVRPGRPVVLKVTNNSTAEHGFEMTDAA
                     NAGAPTVMKAQVVLKPGETKYIGIPTSDMFYATQGNTLSYRCHLHAAHVGGKLLMIK"
     gene            complement(18199..19773)
                     /locus_tag="VLD60_12980"
     CDS             complement(18199..19773)
                     /locus_tag="VLD60_12980"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSB45921.1"
                     /translation="MNHDYVPMHHHPRWFLLLTALITGAVVMALEILGSRLLAPVFGN
                     SLFVWGALIGVILAAMSSGYAFGGWISDRYPGAQVLAGLLLFSGSWTFLIAWLSQPVL
                     FKMAGMIDDPRWGPCAAAAVLLAPPAFGLSGVLPAMLRLAVSDLDYLGRHTGRMIALS
                     TVGSLAGTWGTAFFFLSWIGTQSMVAWLGTIQVALGYWWLLKGISKRPLVVGALALVT
                     LGLGAGAFSPVQMLKAPVYQEDSPYQQVRIRDDDLFRYLVLDRTFHAVMWKADPEALF
                     LPYSQLMVASLALVPEPKRGLILGHGGGSLAKWVASRWPDLELDVVEFDPVVVRMAEE
                     YFSYRAPPQHHVYVRDARVFLNQTNHTYDLIWVDAFARHMIPFHLTTAEFFSELRAHL
                     APDGVLAVNLASSGEGGDLLRANAVVETMRQSFPAIESFAVKGPWKSAQTRAENLIFF
                     AGTPLAHHGISDFVAKVAPLVERQQLPFEAIALLNTHRTQPWDSGVVLTDDYAPYDLL
                     IGSSIQEMQPPAGAIQ"
     gene            19961..21286
                     /locus_tag="VLD60_12985"
     CDS             19961..21286
                     /locus_tag="VLD60_12985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HSB45922.1"
                     /translation="MQISVIGTGYVGLVTGACFAEFGVNVLCMDTDSKRIARLEKGDV
                     PFYEPGITELVAKGIGHGRLSFTTELTRAVDHGEVIFIAVGTPPKKDGSADLSFVEEV
                     GRGIARKMASYKVVVTKSTVPVGTGALLRKVITATQEKKIAFDVVSNPEFLREGSAIE
                     DFMRPNRVVIGSDSERAVALMKDLYRPLYLLETPFVVTDVPTAEMIKYASNAFLATKI
                     SFINEIATLCERVGADVQTVAKGMGLDQRIGSKFLHAGPGFGGSCFPKDLAALVQMGE
                     SAGYPMQIAGAAAAVNAQQRGRMVGKIREALGELQGTTVGLLGLSFKPNTNDLREAPA
                     LSLAEELMGQGVTVRAYDPAALEEACRSLRGIVPCQDAYETARGADALVLMTEWNEFR
                     NLDFDKLKSLMRRTILIDLRNVYESSRVTQFGFRHVSVGRPTKDPDQNV"
     gene            complement(21293..22000)
                     /locus_tag="VLD60_12990"
     CDS             complement(21293..22000)
                     /locus_tag="VLD60_12990"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB45923.1"
                     /translation="MAEEAPVKLIQIGPKGGVKKDGFNLVTERVVAVNPEAKQVEVEL
                     LAYDGKTVVLDVDDEALEELKQLKVGDGATVRVVEEGGKRIAKSFRIRPKDPNAAKAD
                     AMLLDLKDTHWLNRKYAAEVLGELKEPRAVRPLVDALTDEVGDVRQRAYDSLIKIGGA
                     AVPSLVPLLVSEEDELRQSVTEIIRKIGKPAVEPLATALAEADDRLKSRVLKVLDRMG
                     YKPKVKEAAKTEAPRLT"
     gene            complement(22030..22797)
                     /locus_tag="VLD60_12995"
     CDS             complement(22030..22797)
                     /locus_tag="VLD60_12995"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB45924.1"
                     /translation="MSDEGQSDRIEQLIGALRDDNEALREHAIASLSQMGEEALEPLI
                     GLMADEDVLIREAATMAVVRIGPSVVDRLIEALADDEWAIREQAASGLGKLKDPRGVE
                     PLVKALKDKDGAVRTAAVWALERVGDARAVPGLVEALTDSTVREDVARVLKKIGDARA
                     VDALIDGLLGNNWMVRRHAAEALGKIGDQRGIEPLIESLKDEDWLVRRNAAESLARLG
                     AKQAIDPLLPLLEDENTMVQETVEGVLASLGWKQTAQ"
     gene            complement(22790..23887)
                     /locus_tag="VLD60_13000"
     CDS             complement(22790..23887)
                     /locus_tag="VLD60_13000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB45925.1"
                     /translation="MSKESIETLVSELVHEEDWRRMRATAACLAGGPKAVHALVELLR
                     SGSADLKREAAAMLARIKDPQAGVALVGLLEDEDETVRKSGAAALEHMAGILDTETAS
                     ALVALLPKTKEGETRQLVTHLVGAIPTAVTSLCRMMKHPDPEAQVTAATMLDQLLDPR
                     SVDAFIDAMGQPAVRDIAVGTLKKLGAIRERIDQTFDALRDVEGASEREEARMSAVIY
                     LLGIGRPSVEILMEYLEDDDWLVREAAADLLGKIGDVRAVEPLMKRLEQDKDTGVKEL
                     AIKALGLIGDARPTRLYLEAIPIRPLRVYAMEALAKIKDVEVLRPHKALFDRLRTDRD
                     GLVAYNAGLIADKLEALDGAHTASQEGHDNE"
     gene            complement(23919..25043)
                     /locus_tag="VLD60_13005"
     CDS             complement(23919..25043)
                     /locus_tag="VLD60_13005"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB45926.1"
                     /translation="MAVLGCQILGKRNPMADAVAEQIAALNDEDWAIREEAATALGTL
                     RDPRAVQPLVSILRDSDRAVREAAISALTAIGEPSVPALGLCLSDPQLGVQEAASAVL
                     ALIADDRVLAPLIASLGNRDWIVRMHAAKALGRIKQPSSIEPLIPLLQDKVKAVREET
                     TTALAAIGAAAIPSLLAALSHPEWLVRLHAVEALGKMRSPAAVDPLLFVLFNDTDSAI
                     REDAIRALGEIGEGRAVEFLFMVMKEKGLRPLAVEALGRIKDPRAVPVLVEVVRGTNR
                     PEESRPIAGCGDRWDEEMTTMGAAVRALGEIGDPTTIPTLVGALQHTVMRADAAASLV
                     KFGSAVVSFLLPLVNKEQDENIRYHLKETLAQVGWRPGRV"
     gene            25178..>25971
                     /locus_tag="VLD60_13010"
     CDS             25178..>25971
                     /locus_tag="VLD60_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45927.1"
                     /translation="MNHYKHLTNPWIMKCSFFSALFLTGSIAPASAYEVWLTDQSDTG
                     KETGGFLYIYDGAQLAANPTAAKPTQTIDFSGDIGKFCEEATKKAVRRPHMLFFNAKQ
                     DHAIISFLSGHVLFMDAATKKPDACLSMGKNVHAAWPTPDQKMAIAANIAEKKFIRIW
                     TDYAAHKFSFDPEKDVMNLGTMESGERPDTAPICPITESSSRYAFVTLRGGGLLVVDV
                     TATPMKVVATLDNNQVHPAGCGGIESGGTMYINSGGGWPIAPLSYDV"
BASE COUNT         5278 a         8163 c         7014 g         5516 t
ORIGIN      
        1 cgcggctatc ctgaagccat tctgttgttt cgggttggcg acttctacga aatgttttat
       61 gaggatgcgc aagccgcctc caaactgctc tccatcgccc tgacgtcacg agacaagtcg
      121 agcacggatc cggtgccgct ctgcggagtt ccctatcacg cggcgcaggg ctatatcgcc
      181 aagctgctca aggccggacg cacggtcgcc ctgtgcgaac aggtcgagga cccgaagctg
      241 gcgaaaggac tcgtgcgccg cgaagtcgta cgcctctata cgcccggcac actggtcgac
      301 accgaattcc tctctcccgg cgagtcaaac tttctcgcag ccgtcagccg gtctgagcgg
      361 gcaacggccc atcctcagcc tctctgggga ctggctggtc tggacgtatc cacgggtgag
      421 ttttgggtca cggaatttca cggcccggaa gcgacccagc aactcctgga tgagctggcc
      481 cggctcgaac cccgcgagct gcttcatgcg ggacccgcca tcgccgacga actgggtctc
      541 cgccatctgc agggaccgag gctctgcgaa cagtctccgg cgctcttcga tcaccaggaa
      601 gccgagaaat tgttgcgctc gcatttcacc gttcattctc tcgatggctt cggctgccgc
      661 gacttgactg ccggtcttgg agctgccgga gcggtttggc gctatttccg agagactcag
      721 ccgacggctg cgctcgacca tatccgccgc ctccaacggc gctggagcca tgacgcgatg
      781 catttggaca gcgccacgat ccgcaatctc gaactggtgc gtccgatggg tgtggacgag
      841 gaccggacgg gacggcggca gcccaccttg ctctccgtac tggaccgcac gaccacagcg
      901 atggggggcc gcctcctgcg cgattggctc gtgcggccgc tcgtggatcg cagcgccatt
      961 caagccagac tcgaagcagt cgccgaactc aaagagcgcg tcgcccagcg cgtcatgatt
     1021 cgaacgaccc tccgggccgt gcaggatatc gcccgcctca gcagccgcat cacgctcggc
     1081 gtcgcgggac ctcgcgaatt gctggcattg aaacagtccc tgagcgccct gcctgaactg
     1141 cgtgcgcagc tggaatcggt tcaatccgcc ctgctcgccg atcagaagaa ctcatgggac
     1201 gattgccgca atctatatga cgtgatcgaa cagacgctcg atcctgacgc gccgatgtcc
     1261 gcccgcgacg ggggcctgat tcgatcgggt taccatccag gcgtcgacga attgcgcaag
     1321 gcccgaacgg aaggaaagag ctggatagcg gcgctggagg ccaaggaacg ggaacgaaca
     1381 gggatcgact cgctgaaggt ccgctataac caagtgtttg gctactacct cgaggtcacc
     1441 aaggccaacc tctcaaaggt gcctgcggac tacatgcgta agcagacact cgtgaatgcc
     1501 gaacggttca tgacgcccca gttgaaagaa ctcgaagaac gggtcaccgg ggcggaaacc
     1561 aaactggtgg cgctcgaaga agatctcttc gtgcagctgc gcgaacggat tgcgaaagac
     1621 gtaccccggc tgcaaaccat ggcccacact ctcgcgctgc tcgatgtcct ggcctgttta
     1681 gcggaaaccg ccgcattgaa tcgctacgtc cagccaaccc tcgatgaatc gggtatgctt
     1741 ctgattcgag aagggcggca tcccgtcgtt gagcagctga gcccggatct gacattcgtc
     1801 ccgaacgaca cactcctcga cggagaggcc aaccggctcg tcatcctcac cggacccaac
     1861 atggctggca agagcactta cctccgccaa gtcgcgctca tcgtcctcct ggcccagatc
     1921 ggcagtttcg tccccgcgac cgaggcccat gtcggattgg tggaccggat ttttacccgc
     1981 gtcggcgcat ccgataatct cgccgcaggg caaagtacgt ttatggtcga aatgaccgag
     2041 agcgcccata ttctcaactg cgcaaccagc cgaagtctga ttctgctgga tgagatcggc
     2101 cgtggcacca gcacctatga cggcctgagc attgcctggg cgatcgcaga atacatacac
     2161 gaccgccaac acctcggtgc ccggacactc ttcgccaccc actatcacga gatgacgcag
     2221 ctggaatcgt tgcgcgaggg cattaagaac tatagagttg cagtacaaga aagggatggc
     2281 gatgtcgtct tcttgcggaa gatcgtacca ggtggtgcgg atcgaagtta cgggattcat
     2341 gtggctaaac tagcaggatt gccgccgtcc gtgatcgaca gagcccagga agtgctggct
     2401 caactggaac aaactgactc ggcagccgcg acgcccgctc cgtcgtcagc acaagatccg
     2461 ggaagccgtt tgctccccca gtctcacccg atcctcgatg aagtcagaca gatcgacctc
     2521 ttctcgatga ccccgctgga tgcactcaac cgccttgcgg agcttcaaaa gcgcgccgag
     2581 tcagagggca accagcaaac ataaaaaaac ggcagcccct tgcggggctg ccgccttttc
     2641 acattcacac aggtctcgat tagtgaccgg tgttgtattg agctgtccag gggatcaagc
     2701 cagcgagggg cttgccgcct ggttgcagta catcgtactg catcggtggt tgcgcttttc
     2761 ctgcaacgtt aagcgcttgc tttgccgcag cgcagccagc atcgctttca tccttaccgg
     2821 cgcactcctt gaggcggagg gcagaaatgc tccgagtctt tcccggtgca ccagccacct
     2881 cggggagttt cttgacggag gagatcacgc gatggttctg ttccgtttcc gtcgcggtga
     2941 actcaatcag atcagtggtg ctgccgggag gcacttcctt ggcagcggcc ggacctgcgt
     3001 gaggacggcc catcttcttc aactcttccc aggcgccctt atccgcgtaa aacttgaggg
     3061 tcttgcccgg atggatcttc tgccagaaga ggccgctctc ggcattgccg ccgaacacgg
     3121 cgatattaat ccacaccgct tcgtcgtagt gatcaaggac gatcacgcga ccctgcaggg
     3181 tggtgggctt gctcatatcg ccccactcaa aacgctcttg gaatgattcg atcgccagcg
     3241 cggtggaggc cacggagacc accagcgcga ccgcggccac gatggcccag cctttcgatt
     3301 gaaacttcat aatcgcgtcc tccttcaaca gcatgaaaga atataatctt gtcgaagtcg
     3361 attacttgat gaccttgaat ccagtgatcg tccggccatc cagactcact tccatcgcga
     3421 cgagctcctg agaggccttg atgatttgat ccatcaatgc tttgtccttc accgccagcc
     3481 gaactgtcgt ggcggcatgg taccagccgg agtacgggtt gtttctgtca gttccaccaa
     3541 caaatcccca ggcgcagcag ctaaacaacg gatctggcgg cttcacatcg aggtcggagg
     3601 acgaacggcc tgacggagtt cctgaggcgg gctcaccggt gttccaatac tggatcccaa
     3661 agagcaactc gtgatctgga ttgtccgccc actgagacca gacacggcct ttcaaggtct
     3721 tggtcacttc acctttaaac ggcttcccgc cgactatctg atcggctggc ccggcggcac
     3781 cgggagcatc ggcggcaacg gcgaattcgg ccgtcagcaa gccgaacaca gcaaccactg
     3841 caacggcagc tagaagtacg acccttttca tacccaatcc ctccttcata aaagtaacaa
     3901 ccacaaaact gatgaacgcc tcacgtttaa gtacgataaa taatgttgtg aaaccgaatt
     3961 gaggatccgg catccacgag aaacgatgaa cggccacctc ctttgctact tctcgtgccg
     4021 ccttgctttg aatctacagt gatacggcaa aaacgcggtc atggtactga aatcataaaa
     4081 gggagtcaag aaaatgtttg ccctgtggac aacttggaat atgcatgaat tttaaaaata
     4141 aaaacaacat gttacgatgc tgacgatgtt tccattgcag cacgattttg tcgctggaat
     4201 cgacgatcta ttagaggatt ctcatctaaa cgcgtggagc gtgcgggatg tgagcggtcc
     4261 cagtccagta acggcgagat gatccagatc aagcaattga cgaccgacgg tgtagacctg
     4321 ttccgtcgtg actcgatcga tctgtgccaa catgtcttcg agcgaggtgc gcgtcccgta
     4381 caccaactca tctttcgcaa gcttgctcat acggctgtga gaactttcga gactgagcat
     4441 gaggctgccc ttcatctgat tttttgcccg gtcgagttcc ctctgctcga tcccctgccg
     4501 gctgattctt cgaatttccc ggcaaacgag atcgacgacg cgctccacct ctttcggcct
     4561 ggtcgccgca tagaccgtca tggtgccgac atccgaatac ccggagagga aggaatagat
     4621 ggagtagacc aaacctctct tctcccgcac ctcctggaaa agtcgagaac tcatgctgcc
     4681 gcccaggacg ctgttcatcg cgtacaccgc atagcggtct cgatgaccgg ccgccacccc
     4741 tttgagtcca agacagaggt gcacctgctc caacggcttc ttcttcgtga ctaagccgcc
     4801 gcgcacttcc gggggacgcc ttgccgctct taccgacccg tctctccgtt cggtcttgcc
     4861 gaaagaacgg gcgaccattt tcgtcaacgc cgcctgatcg aaatttccgg cgattgcgat
     4921 cacgatctgc gacggatcgt aatgagcgtc gatatatccc atcaggtccc ggcggcgcag
     4981 gccgcggatc gtgtcctcgc gtcccaaaat cgagcggccg agcggatgac ggccgagcac
     5041 ctgtcctgta tgtagttcct gcacgagatc ttccggatcg tcttggacca tccggatctc
     5101 ttcgagcacg acctgctttt ctttttcgac ttccttggga tcgaagcggg aatgatgaaa
     5161 cagatcggcg aggagattga gcgcctgctc gagctgttgg tccagaacct tgacgtagaa
     5221 agtcgtggtc tcgcgcgtcg taaaggcatt catctcgccg ccgagcgcat cgatttcgcg
     5281 cgagatctcg gtggcggacc gggagcgcgt ccccttgaag aacatgtgct cgatgaaatg
     5341 ggaataccct gcctgggagg gttgctcgtc ccgagacccg gtgttgaccc aaatgccaac
     5401 ggtcaccgac ttgagcgtcg gtatccgttc ggtcaccacc cgcacgccgt tgtccaggac
     5461 gagcttgcgg tacaagataa ttacccggag gactgatcct tcgtccccgc tccggttgga
     5521 gccggcatgg tttccttgcg gctaaggcgg atcttgcctt gccgatccac ttccagcacc
     5581 ttcacgagaa tctggtcccc ttccttcact tcgtcggaca ccgctttgac gcgatgatgc
     5641 gcgagttggg agatgtggac cagcccgtct gtcccgggca gcacttccac gaaggcgccg
     5701 aagtccatga tcttccgcac ggtgcccagg tacgtcttgc cgacttccac ctcttcgacg
     5761 atctggttga tcatcccttt cgccttctcc agcgatgcgc cgtcgaccga ggcaatggtg
     5821 actgttcctg tatcgtcgat attgatcttg acgccgcaat ccgcctggat cccgcgaatg
     5881 gtcttgcctc ccggaccgat gacttcgcga atcttgtcct gcttcacctt catcgtgaag
     5941 atgcgcggtg cgaatgcgga catggtcgtc cgcggagctt ccagcgcctt ctgcatacag
     6001 ctgaggatgt gcaggcggcc ggctttggcc tgctccaagg cctggtgcat gagagccggg
     6061 gtaatgccgc cgatcttgat gtccatctga agcgccgtca cgccttgctt cgtcccgcag
     6121 accttgaaat ccatgtcgcc cagatggtcc tcgagtccga gaatgtcgga aaggatcatg
     6181 acccggtcct cttccttgat caggcccatc gcgatcccgg ctaccggttc cttaatcggc
     6241 acgccggcat ccatcatacc caatgtcccg ccgcagaccg tcgccatcga ggacgatccg
     6301 ttggactcca gaatgtctga gacgatacgg agagtatagg ggaatacgtc cttaccgggg
     6361 atcacaggct tcagcgcacg ttctgcgagc gcgccatggc cgacttctcg acgaccgggc
     6421 gaccgcaacg gcctggcttc tccgacgctg aagggaggga agttgtaatg gagcatgaag
     6481 gttctggtgt actcgccttc caaggcatca atccgctgct cgtcatccgt cgtcccgagc
     6541 gtaatgacgg ccaggctctg cgtttcgcct cgggtgaaga gcgccgaacc atgtgtgcga
     6601 ggcaacacgc cgacttcgca ggtgatcggg cggatgtcgg caggaccgcg tccgtcggcc
     6661 cgcgttcccc tctccagaat catgtttcgg acttcggtgt actctaatcc gtggaacacg
     6721 atcttgacgt ggcgttcacg attggggtct tcgggattct tcagcttctc gattgcgtca
     6781 gccaggatca gatccaaccg ctcttgccgc gccgtcttgt tcggaatcat gatggcatcc
     6841 ctgatccctt gcgcgacaag cgccttcacg tggccagtca gcgccggatc gatgtgctct
     6901 gtcacgactg ctcgtttggg cttgttgccc gctgcgaggc ggagctcatt gatcttggcc
     6961 acgatcttct tgatctccgc atgggccaat tcgatcgccg agagcatcgt cgcttccggc
     7021 aattcgttcg ccccggcttc aaccatcatg acggcatcgg ccgttccggc gacgacgagg
     7081 tccaactcgc tgcttttcag tgtctccaaa tcgggattca ccacgaactg tccgctgact
     7141 cggccgatct tgatgccggc cacgggaccc aggaagggaa tgttcgatac ggccagcgct
     7201 gcggaagcgg cggtaatgct gatcacgtca gacgatcctg tcttgtcgga ggacagcacc
     7261 gcggcaatca cctgcgtttc aaaatagtat ccttccggga ataatggacg gaggggacga
     7321 tcgatcaacc ggctggtgag cacttccttt tcggacggcc ggccttcgcg cttgaaatac
     7381 ccgccgggaa tcttgccggc tgcataggcc ttttcctgat agtcgacggt gagagggaga
     7441 aaatcgatac cgggcttggc gttctgcgac gcaacggcgg tggccaagac gacggtgtcg
     7501 ccgtacgagg cccagatcga gccgtccgcc tgtttggcga cgcgacccgt ttcaagccgc
     7561 agggttcggc ctgcgatatc gagttcaact gtgtgtacca tctatggtct ccttctgttg
     7621 tgccccacag atgcccacag agatgccctc cgaaacacgt tgaagtggct cgcggagcgc
     7681 atgtcagtgt tcacctgtgc gactgttctt cccgctgtcg cgcaccggca cgacaacggg
     7741 atctaccggc tactttctga tgcccaatcg gtcgatgacc gcccgatagc gcgcatcgtc
     7801 gatgtcgcgg agataatcca gcaaacggcg gcggcgcccg acgagcgtca acagaccacg
     7861 ccgcgaatgg tggtccttct tgtggagctt gaaatgctcc gtcaaatacg caatccggtt
     7921 tgtcagcact gcgatctgca cttcgggaga gccggagtcg ctgtcgtgtt gccggtattg
     7981 tttgatcaat tccgccttag cttctttcaa taacgccatg ggtaattctc ctcttccttc
     8041 ctttccttcc gactgaacgc taattattcc tggccgacca acactttttc aatcgcaatc
     8101 gacccctgcg gtgcctgcgg ctgtctgccg attgccagca agattccgct gatgtcgtga
     8161 atccgcaccc gcgcgcccgg tttctgatcc acgttcccag ccgactccca gcgcaaaacc
     8221 ttggaagcag gaaccgggac cccgtgacgc acacggtcgg ctgtctgtgc atccaccact
     8281 accgccggga gtccctgcaa agcttgatcg agagactgca cgtcctctcc taaacgacca
     8341 agcgccagat gggcctccac ttcatccacg gacacagcct gatcaaaggt caggggcccc
     8401 acccgacggc gctccagcgc aagtaaatgc ccgccgactc ccagggcctc tccgatatcg
     8461 gcacagagtg tgcggatata ggtgcctttg gaacaggcca cccgcaaggt cacgtcgcgc
     8521 ccgtcaatgg ctaggatgtc gatcgaatgg atcacgatct gcctggcttc ccgcgcgatc
     8581 gttttccccg cacgggctga tttgtagagc ggcacgccag cgaccttgac ggctgaatac
     8641 atcgggggaa cctgttcaat ccggccgcgg aactgcccga ctgcttcctc gatcgccgct
     8701 gacgtgactc cgtcagttgc acaccgaacc agcacggttc ctgtcgcatc ctgcgtatca
     8761 gtcgtctctc cgagacgaag gatagccaga tactccttgt cccattccat cagatactcg
     8821 gcgatgcgag tccctttccc gatcaggaca ggcagcacgc cggtcgcggc cggatccaat
     8881 gttcccgcat gaccgacttt aatgccgccg agaagatgcc ggatcttggc cacgacatcg
     8941 tgagaggtcc aaccggcttc tttcttgatc accaggacgc cgtccatcac gccgcgccct
     9001 tggatggttc cattcatgcg tacaccgact cgttcgatta cgaactgacc ggatcctggg
     9061 gagcgtctcc ctcggcgtcc gtcttaacgt ggatctcgtc cagcaactgc agcacacgat
     9121 cacctcgcgg cccgctgatg tctttgacga agaccacttc aggcaggtac cgcagcgtca
     9181 ggcgacggcc cagttcgctg cgcacaaatc cgctcgcctt agccaggccg gcgaagacat
     9241 ccttggtttc agcatccgtc cccaacgccg tgacaaaaac ccgggcgatg cgcaggtcat
     9301 tcgtcagttc gacgtcggtc acggtcaccg agcggacgcg cgggtctttg atcttccgca
     9361 tgaggatgtc ggccacctcc atgcggatct gatcggcgac gcgatcggct cggctatacc
     9421 ccgtcttggc catcgtccgt tccccttggc cggcccgcgc gcgcaggcct ttcggcgtca
     9481 caacaattcg actcgagact ggacgatctc cacagccggg tcgctgcgga tcatgttcag
     9541 tgcctggtcc aacacctgat tcacataccg gccgtcgttc gccacgcagg ccagcccaag
     9601 caccgccttc tgccacaaat cctgtccgtc gacttccgcg acggacaggt tgaatttctg
     9661 atgcagccga tccttgacgc tcaacaggac ctgccgcttg tctttcaacg actggctgtc
     9721 cggtaagaac aactcgatcg tgcagagccc gacgatgatg ctcacgatcc gatcggctca
     9781 cgccttagag tttcgccgcg atcttatcga tcgcgtaggc ttcgatgatg tccccggtct
     9841 tgacgtcgtt gaaattctcg acaccgattc cgcactcgta cccctgctgc acttcacgaa
     9901 cgtcatcctt gaaacgccgg agcgatccta acttgccctg atagaccacc acattgtcgc
     9961 gaatcactcg gacaccggcg ctggcgcggg taatggctcc gtcgatgaca tacgacccgg
    10021 cgatcgtacc ggccttggaa accgtaaaca cctgacgcac ttcggcccgg cccagcactc
    10081 gctccttgag cgtcggttcc aacaggcctt ccatggccgc tttgatgtcg ttcaacgcat
    10141 cgtagatgat ggtgtacagg cggatgtcga cccgttcctt ctccgccagg gcggaggcct
    10201 tgggctccgg tctgatattg aatccgatga tgatggcgtt cgaggcggca gccagcaaga
    10261 cgtcggattc cgtgatcccg ccgacaccgt tgtggatgac cttgagcttg accatgtcgg
    10321 tcggaagctt ttcaatcgcc gcggccaagg cttccgatga accttgcacg tcggccttga
    10381 tgaccagcga caattccttg accgaacctt cctgaatttt ggcgaagagg tcgtcgagcg
    10441 agaccttccc ggctccggcc agttctgcag cccgctgctt acgcgcccgg tcctccgcga
    10501 tctcgcgcgc aacccgttcg tccttcacaa cctgaaacac gtcgcccgca gacggcacac
    10561 cgggtaatcc attgacttcg actggaatcg aagggccggc ctcctgcact tttgtgccgg
    10621 tatgactgat caatgcccga accttgccgc tgaaagatcc cacgacgaac acatcgccga
    10681 catgcaacgt tccgctttgc accaacaccg tcgcgacggg accacggccc cgctcgagct
    10741 tggcctcgac caccgcgcct ttcgccaact ggtgaggatc cgctttcaat tccaacactt
    10801 cagcctgcag gagaatcatt tccaacagct ggtccaaacc cgtcttttgc ttggctgata
    10861 cttcaaccat gatcgtgtcg ccgccccagg cttcggaaat caatccgtgc tcggacagcg
    10921 catttttcac tcgatccgga ttggcgccag gcttatcgat cttgttgatg gccacgatga
    10981 gcgggacacc ggcggccttg gcatggtgaa tggcttcgat cgtctgaggc atgacgccgt
    11041 cgtcggccgc cacgacaagg atcacgatgt ccgtgacctt tgctccccgg gcacgcatcg
    11101 cggtaaaggc ttcgtggccc ggcgtatcga ggaaggtgac ctgcttgccg cgcaccgaga
    11161 ccgtataggc tccaatatgc tgcgtgatgc cgccggcttc gccttccgcc accttcgttt
    11221 cacggatcgc gtcgagcaac gaggtcttgc cgtggtcgac gtggcccatg atcgtcacaa
    11281 ccggaggacg cggttccgga cgggcagtgc cttcggtctg aatcaccgtc tccaacaaat
    11341 cttcaccgcc cttctccacc gccacgtcca ttttgatccc ggattcttcc gcgatcattg
    11401 aggcgacatc aacattcatc ggttgattga acgtcagcat ctgacccatg tccatcaact
    11461 tgcgcacaat gtcggcgggg cgctgtccga tcaattctgc gaattccttc accgtcatgc
    11521 cgggtgtgag cttcacgctc ttcttgcggg gcttggtaat ttccgccggc gtgctgtgat
    11581 gcacgtgttt ggcacgatcg tcccgtcgtt gcaccgggat ggcgcgcaga tcctgccagc
    11641 gcgccgcatc ttcgcgtacg cggacgtcgt cttcttcacg aacacgtccc ggtttgcggg
    11701 ctttcttgag cttatccttc agaccctcag cctgaagctc ttcgaagact ttcttctttc
    11761 cggaaagagg ttcgatggtt ggcgctacgg caggcttagc agccggtggc tgctccagcg
    11821 tcggcgccgc aggcgacgga atgagtccgc tgggcggatg cacctcgccg gaaggggcca
    11881 tatggctttc gacatgatgc gagggcggcg taacggacgg cgctgccaca gcttccgtcg
    11941 cagccgcggc cagagattct gacccgagcg gagccaggga ctcctcgagc ggcgcttctt
    12001 cgctacgttt tcgcttaatc agaatacggc gcttgtcagg cttggattct tccgctacaa
    12061 ccggatgggc tccggcggtt ttcactccgg cgtgatgagg cacgcctgca tcggtgtctt
    12121 tgtgcttctc ggtgcgtgcc gtatctcctt cgcctgcttt cgtcacaatc tttgctgcgg
    12181 tcttgtgctt cgggcccagc ttctccagca ccttttgaac ggcatcgtcc tccaatgcgc
    12241 tgctgtgaga cgcaacggga atacccaatc gcttcaactc cgggatcaga tcgcggtttt
    12301 ccatcccgag ctgtttggct aattcataga cacgcatagc gcttcaacac ctgctccaat
    12361 gggactccct actacccctc cgactcggct tgggccttct cctgctgtcg agcctcttcc
    12421 tgctgccgct gggcttcggc ctcttcggcc aatgcggcct tgatttcctt gtcccgctcg
    12481 gccttttctt tttcgtactc cgtggcgctg atgatgtcga tcttccagcc ggttaaccgc
    12541 gccgcgagcc gcacattctg cccattcttg ccgatggcca aggacaactg cgagtctgcc
    12601 acgacgacca atgccgactt cttttcttcg tcgacgccga ctttttcgat ggtcgcagga
    12661 ttcaacgctt cggcgatgaa cacacgcgga tccgacgtcc aggtaatgat gtcgattttt
    12721 tctccgcgca gttcccgcac aacggcctgc acgcgcgaac ccttgatgcc gacacaggcc
    12781 ccgaccggat cgaccgcttt ctcgcgggag gtgacggcga tcttggtgcg atcacccggc
    12841 tcccggacca cggccttgat ctcgacgatt ttttccatca cttcaggcac ttccagctcg
    12901 aacagtttgg cgacgaactg cggatgactc cgcgtgagaa tgacttgaac gtctttcggc
    12961 gtccgacgca cttccaggag catcgctttc acgcggtcgc cgcggcgata agtctcgcga
    13021 ggaatttgtt cctggatcgg aagaatcgct tccgtcttcc ccagatccac gagatagttc
    13081 cggcgttcca tcccgagaat gatcccgttg accaaatcgc cctgtcgcgt cgagtattcc
    13141 ttctgaaccg cttcccactc ggcctcgcga actttctgga agatcacctg cttggcggtt
    13201 tgtgccgcaa tacggccgag ctcgtccatt tcgataagcg agccgatttc gtcgccgatt
    13261 tccgcttcgc tgtcataggc ccgagcttct ttgagtgaga tctcggcctt cggattggtc
    13321 actgaatcga cgatgatctt cttcgagacg acggaaattt ctccggtctt gggatcgatc
    13381 tcgacctgga tattttcggc ctgcccgaag cgcttcttgg cggctgtttg aagagcggcc
    13441 tcaatggcac cgatgacctt gctcttatcg atgccttttt gtcgcccgat ctcgtcgatg
    13501 actgaaatca attctcgatt catgattcgc tctccactct agcgccgtcg acgcttacca
    13561 cgtcactttc aatcttgcct cggcaatggc tccatactcg agggtcaacg tgcgcaccgg
    13621 aggcggatcg cttacgacca acgccacagc gtgctccgtt acttccgtca atgttcccac
    13681 caggcgccac tgcccctcga gcggctctct gagcttgacg ctcactcgct tcccgactgc
    13741 ccggcgataa tcctgaatcc gtttgaaggg ccgatccaga cctggcgagg aaacttcaag
    13801 cgtgtaggca tggggaaacg ggtcggcaac atcgagcgct gggccgagcg ccttgtgggc
    13861 gcgctcgcaa tcgtccagtg ttacgccacc cggcttatca atgaataccc ggaccaccga
    13921 acgcggcccc tgccccacgc acacaacgtc aaccaattcc aggcctaacg tccagaggat
    13981 aggtgatact gcttcactga tacggtcgac gaccggtcgg gactccttca ccgacacccc
    14041 tagccccgtt tctctggctg ttctgttcac gagatcaaac aaaaaagtgg gcctgagccc
    14101 acttccagcg gaggaaccat agcatgtcac ccctgccaaa gcaagggggc ggactagccg
    14161 atccgctcga cgaacctcct ggcgagccgt tgggtaagag ggcccggctt cccctctccg
    14221 atcgccttgc cgtcgattcg gacaactcct aacacctcta ccgtcgtccc ggtcaaaaag
    14281 acctccgccg cgtcgtacag ctcctgtctg gccacatagc gctcctggac cgggaaacct
    14341 tcctgtcgag caagctggag cacgacggct ctcgtcaccc cggacagaat gcaaggcccc
    14401 tccggcgccg tgatcaccat cccatcccgc accaccatca cattactcac cgccccctcg
    14461 gtgacctctt cacctttcac cagaatggct tcaaacatct gcgcctgctt ggcctgttgc
    14521 cgggcaagca cgttggccag cagattaatg ctcttgatgt cgcagcgccc ccatcggatg
    14581 tcctcgatgg tcatggcgtc gacaccggcg gcctgcacgg attgactgag tggacgcagc
    14641 tcacgcaccg tcatcacgac ggttggcacg agatccgcgg cataggcatg gtcgcgcggc
    14701 gcaacgcctc gggtgatttg aagatagacc ttcgcctccg gaaaggccgc ctgcttgatc
    14761 ccttccagaa tgagctggtg ccactgctga tgggaatagg gctgcgggag atccagccct
    14821 cgagcgctcc ggtttaagcg ggtcaggtgc gcatcgagct caaaggggcg actgcgatat
    14881 gtcctgatga cctcatagac accgtcgccg aatagaaagc cgcgatcctc aatcgagatc
    14941 atcgcgtcgg ccagcggcat aaaccgtccg ttgacgaggg cgatatccgg cacgattact
    15001 tgctccgtga ggacgagggt tcggagaccg tcacctgaaa gacgcgccgg tcttccgcag
    15061 tgaacttcca tcccatgcgc ttttcaaaca cgatttggta gcttcccggc ttgacggctt
    15121 tgaactcgaa ggttcgacgc ccgttatcga cggcattgtt gcccgcgatg cggagatagt
    15181 catcggccaa caatgccagg cccgtcgtat catacgtcgg aacccagagt tctcctctcg
    15241 tccggtcttc ccacaagtgg atctcgatag ggccgtcgag ggtggcttcg atcatcttgg
    15301 catgatcctg cggcctggca cctccatctg tcatgctcat ccgtctatcc tcaaaccctt
    15361 ccttgttcca taccgattca gtgagcccga cgtgacttgc tcgagtcctc cactatgttt
    15421 ttcgccttgg accaacgaag atctcctcgc cctccacacg aacctcgtag ctgccgatgc
    15481 agtacccgcc cccgtccgtc ccctgtccgt tgcgtatatc gaacgccaga tcatgccatg
    15541 gacaagacac tacaaatcct ttgaccctgc cttcattcaa cggaccgccc tcgtgtgggc
    15601 agaggttgtg gatggcgaaa tactttccct cgatgttgaa caacgcgatg gcgcgattgt
    15661 tcaccgtgac caccttcgat tgaccgggcg gcacttcgcc gactttggcg accaattgaa
    15721 atccttcgct tgccacgctc ctacttcctc cttctcatgg cgacgatagc ccgcatcaca
    15781 tgcgcagcgt atatttcaat tcacccccga cgacaagacc ctgccgcttc gcgaaaccgg
    15841 cctccgtctg gatcaggtac cggacgacct ccgtcgttga gccgtgccag ggacaagtct
    15901 cctgacggca tggcggcaca ttctccgtga tcgacacgat gtgatgactc tcatcgaccc
    15961 agatcatgtc gacgggaatt cgatattctt tggttgtcac gcgatgaggg ccggtcgcct
    16021 cgaagatata aatcattcct ccgttcgggg gcaatgcctc ctgaaacgcc aatccgaaga
    16081 ggagtttctc gggagtgtcg gcaacttccg cctcgaggtc ggttccgttc ggaaaggtca
    16141 cgatgatggt cttcgaatct tcccgttgca tcacaaacat ggagacactc atcaacagaa
    16201 tcgccagcag gatcatcatg atgatccgct tctttttctg ttctcgttcg ctggtgggaa
    16261 ttgaggccat agggatctcg cgagggcgac gcaacctggg cccaaaggcc catcatatca
    16321 gggaatctag gccttctgac cgaaggccgt tctgttgact tggaaaagag cggcggacta
    16381 gaatgcgctc acgtcgcgtc acgcaatatg gtcgtgacgg ggcgggcttg tcaaccgtgt
    16441 ttgtcgctat atttgtctct attgtgggga gaaggaggcg agggtcatgg cactgctgat
    16501 taccgatgaa tgtatctcct gtggagcctg cctacccgaa tgtccgaacg aggcgatttt
    16561 tgaaactcgc agcgacgcgg aatcgaaggg aaaccatgtg ggcgatggcc agggtgtcgg
    16621 cgacaatatt tacgtaatta cgcacgatcg ctgcacggaa tgcgtcgggc atttcgacga
    16681 accgcaatgt gccgccgtct gtccagtcga caactgctgc atctccgacc cggcctatcc
    16741 ggaaacgacg gatgtcctgc ttgaaaaagc gaagacactg aatccggaca agactataga
    16801 tgcagcgaag gtctggagtg gcgtccgaaa ctaaagcctg atctcagcac tcacaaaggc
    16861 cctgcctccc agaacggagg tagggctttt gcgtcttacc ttcacacgta cgttttccac
    16921 ctaattagta gcattctcgc cgacctcgct gtagaatttt actgtagtag cggttttgac
    16981 tggtcaggct cctacgtctg catggagccg cctgtgcttc tcacctcgcg tggaggtcac
    17041 accatgcggt accgaggcat cctcattgga tcgttcctcg tgctcctcat ccttgcaccc
    17101 ttggctcagg ccagcaccgg agatatcgga tcgatcatcg agaagttcgt ggaccgacag
    17161 tttcctcatg cggccgcgca ctactgggtg atcaacgaga ctcagtggga cggagacgag
    17221 atggtcgtgg acgtccacac gattgtaaaa gagcggcggg atactgaacc catattaaac
    17281 cgttttctgc tgttgatcgt ggcaggagag gtcaaagggg tccaaaacgt gcctcttgaa
    17341 ccgggcaccg attgtcgcgc cgaagaagac gtgtaaccat cggcacatag gctgccaaag
    17401 caacaaggcc ccctccgttg tgatagcgga gggggccttg tcatatcgtt cgagttaccg
    17461 actcgattac ttgatcatga gcaacttgcc gccgacatgc gccgcatgga ggtggcagcg
    17521 atagcttaat gtattaccct gcgtggcata gaacatgtcg ctggtcggaa tgccgatata
    17581 ctttgtctct cccggcttca gtacaacctg agctttcatc acagtcggag ctccggcatt
    17641 cgccgcatcg gtcatctcaa atccgtgctc agcagtggaa ttgttcgtca ctttgagcac
    17701 gaccggacga ccaggccgaa ctttgaaatc gataatcgtg actggtggat accaggtctt
    17761 catgttcccg atctcgatcg caaacaactg ggcatccact tccaattcct tgaacggttg
    17821 tcccacgaca gacccagtct ccagatcccc gatttcgact ccgccagcct ggagcgcgaa
    17881 cgctgcagac gccccagcca cgaccatcac gaggccgaag aagaccccta acatcgtctt
    17941 gcccatgacc tacctcctta aaaagaagaa gagatgtgat taggttggta aataactacc
    18001 gctacagtca ggtagccttg accgctctga caacgactga cctgattcat tcgattcacg
    18061 ttcatagtct tctttgagag gcaaccttat agcacagcct tgaaaacgga agcaagaagg
    18121 ttaatgcgca ttaactcggg tggttcacac aaattcacac tgaactatca acagggaaat
    18181 agcaacacat tcaatgcctt actgaatcgc tcccgctgga ggctgcatct cctgaatgct
    18241 cgatcctatc aaaagatcgt acggtgcata gtcgtcggtc aggacaacac cggagtccca
    18301 gggttgggtg cgatgcgtgt ttagtaaagc aatggcttcg aagggaagct gctgtcgttc
    18361 aacaagcgga gccaccttag cgacaaagtc ggatataccg tgatgtgcca acggggtgcc
    18421 ggcgaaaaag atgagatttt ctgctcgagt ttgagcggac ttccaaggcc ccttgaccgc
    18481 gaaagattcg atagccggaa acgactgtcg cattgtctcc accaccgcgt tggcccgtaa
    18541 gaggtcccct ccttcaccag atgacgcaag attgacggcg agaactccgt ccggagcaag
    18601 gtgagcccgc aactcggaga aaaactccgc cgtggtgaga tggaaaggaa tcatgtggcg
    18661 cgcaaatgcg tctacccaga tcaggtcata tgtatggttc gtctggttca ggaacaccct
    18721 ggcatctcgc acatagacgt gatgttgagg aggggcgcgg taagaaaaat attcttctgc
    18781 catccgtacg acgaccgggt cgaactccac gacgtccaat tccagatccg gccagcgact
    18841 ggccacccat ttggctaacg aaccgcctcc gtgaccgaga atgagacctc gtttgggttc
    18901 aggaaccaag gccagggagg ccaccatcag ttggctgtag ggaagaaata atgcttcggg
    18961 atctgccttc cacataacgg catggaaggt ccgatccagg acaagataac gaaagagatc
    19021 gtcgtctcgg atacggactt gttgataggg actatcttcc tggtagaccg gagccttcaa
    19081 catctgaacg ggagagaaag cgccggcacc aagaccgagg gtaaccagcg ccagtgcacc
    19141 cacgacgagt ggccgtttgg atattccctt aagtagccac cagtagccca atgccacctg
    19201 gatcgttcca agccaggcca ccatcgactg cgtgccgatc cacgacaaaa agaagaaggc
    19261 tgtcccccac gttccggcca aacttccaac agtcgagagg gcgatcatgc ggccggtgtg
    19321 tcgaccgagg tagtcgagat cggagacagc caatcgcagc atcgccggca gcacgccgct
    19381 gaggccgaat gccggaggag ccagcaaaac cgctgcggca gcgcaaggtc cccaccgggg
    19441 atcgtcgatc atcccggcca tcttaaaaag caccggctgg ctcagccagg cgataaggaa
    19501 cgtccaggat ccggaaaaca acagcaaccc cgccaacacc tgcgccccag gatagcggtc
    19561 cgagatccac ccaccaaaag catagccgct gctcatggca gccaaaatga cgccgatcaa
    19621 cgcgccccac acaaagaggg agttaccgaa caccggcgcc aacagccggc tccccagaat
    19681 ttcaagcgcc atcaccacgg cacccgtgat gagcgccgtc aagaggagga accagcgagg
    19741 atgatgatgc atcggaacgt aatcgtgatt catgtagagg gatcgaaacg caacttgggg
    19801 gctgtccgag tggtcataaa tttcgttgcg aggcggcata gtactcagcc ggtcacagac
    19861 ctgtcaacgc atcgaaatgt ggcaacttcg ttgctcggat tggagaagca cgctatactg
    19921 cacaaattat tattgccgct gaatagaggg agaaccgtcg atgcagataa gtgtcattgg
    19981 aaccggatat gtcgggcttg tcacgggcgc ctgctttgct gaatttggcg tcaacgtgct
    20041 ctgcatggat accgattcaa agcggatcgc tcgactcgaa aaaggcgacg taccctttta
    20101 tgagccaggc atcactgagc tcgtggcaaa ggggatcggc cacggccggc tcagtttcac
    20161 aacggaactc acgcgggccg tcgatcatgg cgaagtaatt ttcatcgccg tcggcacacc
    20221 cccgaaaaaa gacggatccg ccgatctttc gtttgtcgaa gaagtgggcc gcggcattgc
    20281 ccggaagatg gcgtcctata aggtggttgt cacgaagtcc accgtgccgg tcggcactgg
    20341 ggctctcttg cggaaagtca ttacggcgac ccaggagaaa aagattgcct tcgatgtcgt
    20401 gtccaacccc gaatttctcc gggaggggtc ggccatcgaa gatttcatgc gccccaaccg
    20461 tgtcgtgatc ggctcggaca gcgagcgcgc cgttgcgctc atgaaggacc tctatcggcc
    20521 gctctacttg ctcgaaacgc cgtttgtggt gaccgacgtt cccacggcgg aaatgatcaa
    20581 gtacgcgtca aatgcatttc tggcgacgaa aatctcgttc atcaacgaaa tcgcgacgct
    20641 ttgcgagcgc gtcggagccg acgttcagac cgtggcgaaa ggcatgggct tggaccagcg
    20701 aatcggctcg aagtttctcc acgctggtcc cggattcggc ggatcctgct ttcccaaaga
    20761 tctagccgcg ctcgtccaaa tgggcgagag cgccggctat cccatgcaga tcgctggagc
    20821 cgctgccgcc gtcaacgccc aacaacgcgg ccgcatggtg ggaaagatta gagaggctct
    20881 cggtgaactg caaggaacga ccgtagggct actcggcctg tccttcaaac ccaataccaa
    20941 cgacctgagg gaagccccgg ccctgtctct cgccgaagag ctgatgggtc agggcgtcac
    21001 cgttcgggcc tacgacccgg cggcacttga ggaagcttgt cgatcattac gcgggatcgt
    21061 gccctgccag gacgcctatg agacggcaag aggcgctgat gcgctggtgc tgatgactga
    21121 gtggaacgaa ttcaggaacc tcgacttcga caagctgaaa tctctcatgc ggagaacgat
    21181 tctcatcgac cttcgcaacg tctacgaatc cagtcgggtc acgcaattcg gcttccgcca
    21241 cgtctcggtc ggacggccaa cgaaagatcc cgaccagaac gtctagcctg gcctatgtca
    21301 gccgaggcgc ttctgttttc gccgcctctt tcactttcgg cttgtatccc atccggtcca
    21361 ggactttcag cacacgactc ttcaaccggt cgtcggcttc cgccaaggcg gtcgccagcg
    21421 gctcaaccgc cggcttcccg attttccgaa tgatctccgt caccgattgc cggagttcgt
    21481 cctcctcgga aacgagcaac ggtaccagcg aagggacggc cgctccccct atcttgatca
    21541 acgaatcgta cgcgcgctgc ctgacgtcac cgacctcgtc tgtcagagca tccaccagcg
    21601 gtcgtaccgc cctgggctcc ttcaactcgc cgaggacttc cgcagcatac tttcgattca
    21661 gccaatgggt gtctttcagg tccagcagca tggcatcggc cttagcggca ttgggatcct
    21721 tgggacggat tctgaaactc ttggcgatcc gctttccgcc ctcttcaacc acacgaacgg
    21781 tggcgccatc cccgaccttg agctgcttca gttcctcgag cgcctcatcg tcgacatcca
    21841 gcacgactgt cttgccgtcg taggccagca attccacttc aacctgcttg gcctccggat
    21901 taacggccac aactcgctcg gtcacgagat tgaatccgtc ttttttcaca ccgcccttcg
    21961 gaccgatctg aatgagtttc acaggagctt cttctgccat ggtgtgcgtc ctttacattg
    22021 aaatgtagat tactgagccg tttgcttcca tcccaagctt gcgagcacgc cctctacggt
    22081 ttcctgcacc atcgtgttct cgtcctcaag gagcggcagc aagggatcaa tcgcctgttt
    22141 cgcacccagc cgggcaagcg actccgctgc attcctcctg acaagccaat cttcgtcctt
    22201 gagtgattcg atcaacggtt cgatcccgcg ctgatcgccg atctttccga gcgcctcggc
    22261 ggcatggcgc cggaccatcc agttattccc cagcaatccg tcgatcagcg catccacggc
    22321 gcgggcatct ccgatcttct tgagcacacg ggcaacatcc tctcgcacgg tgctgtccgt
    22381 caaggcctcg acgagccccg gcacggcccg tgcgtctcca acccgttcga gcgcccagac
    22441 ggctgcagtc cgaaccgcgc cgtccttatc cttgagcgcc ttcaccagcg gctcgacacc
    22501 tcgcggatct ttcagcttcc ccagtcccga ggcggcctgt tcgcggatcg cccattcatc
    22561 gtctgccaga gcctcaatca accggtcgac cactgaaggc ccgatgcgga ccaccgccat
    22621 cgttgcggct tcgcggatca aaacatcttc gtccgccatg aggccgatca gcggttccag
    22681 cgcctcctca cccatttgac tgagacttgc gatggcatgc tcgcgcaatg cttcgttgtc
    22741 atcgcgaagc gcgccgatta attgctcaat cctatccgac tgtccctcgt cactcattat
    22801 catgtccttc ctgactcgcc gtatgggcac cgtcgagcgc ctccaatttg tcggcaatga
    22861 gcccggcatt gtaggcgacc aacccgtcgc gatccgttct caatcgatcg aacaacgcct
    22921 tgtgggggcg caacacctcc acatccttga tcttggccaa agcttccatg gcatagacgc
    22981 gcagcggccg gatcgggatg gcttcgagat agagccgggt cgggcgggca tcgccgatga
    23041 ggccgagcgc cttgatcgcc aactccttta ccccggtatc cttgtcctgc tccagtcgtt
    23101 tcatcaacgg ttccacagcc cgcacatcgc cgattttccc gagcaaatcc gccgccgctt
    23161 cacgcacaag ccagtcgtcg tcctctaagt attccatcaa aatttccacg ctcggacggc
    23221 cgatgccgag aagatagatc acggccgaca tccgcgcctc ttcccgttcg ctcgctccct
    23281 cgacgtcgcg cagcgcgtcg aacgtctgat caatccgttc gcggatcgcg ccgagtttct
    23341 tcagcgtgcc cacggcaatg tcacgaaccg ccggctgacc catggcgtcg ataaaggcat
    23401 ccaccgagcg cggatccagc agttgatcga gcatcgtcgc cgccgtgacc tgagcttccg
    23461 gatccggatg cttcatcatc cgacagagcg acgtcaccgc ggttgggatc gccccgacaa
    23521 gatgtgtcac gagttgcctg gtctcccctt ctttggtctt gggaagcaga gccaccaatg
    23581 ccgaggcggt ctccgtgtcc agtatccctg ccatgtgttc caacgccgct gctcccgact
    23641 ttcttacggt ttcatcttcg tcttcaagaa gaccgacgag cgccacgccc gcttgaggat
    23701 ccttaatccg tgccaacatg gctgcagcct cacgcttcaa atcagccgac ccggaacgaa
    23761 ggagttcgac aagcgcatgg accgctttgg ggcctccggc aaggcaggcc gccgtggctc
    23821 gcatccgccg ccagtcctcc tcgtggacga gctcggacac cagcgtttcg atgctttcct
    23881 ttgacatgaa ctccgtccgt cagactaaga ggttcgacct acacccgccc aggccgccaa
    23941 cccacctgcg ccaacgtttc ttttagatga taccgaatgt tttcatcctg ttctttgttc
    24001 acgagcggca acagaaacga caccacggcc gacccaaact tcacaaggga agcggctgca
    24061 tcggctcgca tgaccgtgtg ttggagagcc ccgaccaacg tcgggatggt cgtgggatcg
    24121 ccgatctcac cgagtgcccg cactgccgcc cccatcgtcg tcatttcctc atcccaccgg
    24181 tcgccgcagc cggcaatcgg ccggctttct tccggacgat ttgtgcctcg aaccacctcg
    24241 accagtacgg ggacggcgcg aggatccttg atccgcccca gcgcttcgac tgctaaaggg
    24301 cgcaatccct tttctttcat gaccatgaac agaaattcga cggcgcggcc ttctccgatt
    24361 tcacccagcg cacggattgc atcctctcgg atcgccgaat ctgtatcatt aaacagcacg
    24421 aacaacagcg ggtccactgc cgccggtgat cgcattttcc ccagagcttc cactgcatgg
    24481 agccgtacaa gccattccgg atggctgagc gctgccaaga gtgacgggat ggccgccgcg
    24541 ccgatagcgg caagcgccgt cgtcgtctct tcgcgcaccg ccttgacctt gtcctgcaac
    24601 agagggatca agggttcgat ggagctgggc tgtttgatac gccccagcgc cttggcggcg
    24661 tgcatccgaa cgatccaatc cctattgcca agtgatgcga taaggggcgc cagcacgcga
    24721 tcgtccgcaa tgagcgccaa caccgccgaa gccgcttcct gcactcccag ctgcgggtct
    24781 gagaggcaga gccctaaggc cgggacggat ggctcgccga tcgcggtcaa cgcactgatc
    24841 gccgcctccc gaacggctcg atcactatcc cgaagaatgg agaccagcgg ctgcacggca
    24901 cggggatccc ggagagttcc cagagcggtt gcagcttctt cgcgaatagc ccaatcctca
    24961 tcgttcaggg ccgcaatttg ctctgcaacc gcatcggcca tgggattcct cttgcctaga
    25021 atttggcagc ctaacacagc cattttcaca gggtcaacgg aactgcagcc acccaagtcc
    25081 gcagcgattc ggcgtgattg actccttgga gatcgccccg taccatcaca ttcccatctc
    25141 ttcacacgcg ttacgtgtct tgcccagagg aggcctcatg aaccactaca agcacttgac
    25201 caacccatgg atcatgaagt gctcgttctt tagcgccctg tttttgaccg gtagcatcgc
    25261 cccggcttca gcctacgaag tctggctgac cgatcaatcg gataccggca aggagaccgg
    25321 cggtttcctg tacatttacg atggcgcaca actggcggct aatcccaccg cagctaagcc
    25381 gacccagacc attgatttct caggtgacat tggcaagttc tgcgaggaag cgaccaagaa
    25441 ggcggttcgc cgcccgcaca tgcttttttt caacgcgaaa caggatcatg cgatcatctc
    25501 gttcctgagc gggcacgtcc tcttcatgga cgcagcgacc aagaagccgg acgcctgcct
    25561 ctccatgggc aaaaatgtcc atgccgcctg gccgaccccg gatcagaaaa tggcgatcgc
    25621 ggcgaatatt gccgagaaaa agttcatccg aatctggacc gactatgcag cccacaagtt
    25681 tagcttcgat cccgagaagg acgtgatgaa cctcggcacg atggaaagcg gagaacggcc
    25741 cgataccgca ccgatttgcc ccatcacgga atcctccagc agatacgcct tcgtcacact
    25801 acgcggcgga ggactgctcg tagtcgatgt caccgcaacc ccgatgaaag tcgtggccac
    25861 gctcgataat aaccaagtgc acccggccgg ctgcggcgga atcgaatccg gcgggacgat
    25921 gtacatcaat tccggcggcg gatggccgat agccccgctg tcgtacgatg t
//
LOCUS       DATIFR010000226         1639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1114399, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000226 DATIFR010000000
VERSION     DATIFR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114399"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1314)
                     /locus_tag="VLD60_13015"
     CDS             complement(226..1314)
                     /locus_tag="VLD60_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSB45928.1"
                     /translation="MRTGRPKKPLEIADEDREKLSMIARRPKSAQAMAMRARIVLSCG
                     QGMSNSEVARKLHITGATVGKWRERFREFGMDGLLDEPRVGAPRKISDRQIEEVVTKT
                     LESMPANSTHWSTRRMAEETGLSQNAIVRIWRAFGLQPHRVENFKFSKDPQFVEKVRD
                     IVGLYLNPPDRAIVLCVDEKSQVQALNRTEPILPLAPGIPARQSHDYERHGVTSLFAA
                     MDVASGVTISNCYRRHRHQEFLRFLNEIEGNLPAGLEVHLVMDNYGTHKVAKVRSWLA
                     RHPRYRVHFTPTSGSWLNLVERLFAEVTERCVRRGSHTAVRVLEKAMLDYLDQRNENP
                     KPFVWTADADLILGKVERLCKRISRSGH"
     gene            complement(1369..>1639)
                     /locus_tag="VLD60_13020"
     CDS             complement(1369..>1639)
                     /locus_tag="VLD60_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB45929.1"
                     /translation="TATEFRLLEFLMGRPEVVFNREQLLDAVWGHDRAVTDRTVDVYI
                     LRLRQKIEADPANPRMIRSVRGFGYSFAVDAKETASTHSPAVTHN"
BASE COUNT          328 a          504 c          448 g          359 t
ORIGIN      
        1 tctttggctt ggcaccttta tttcaatcct gcgaaccgtg gcccggcaca agggtgtgcc
       61 tcgtgacgca atcatcgcaa ctttgtagga caccggaggc caatagccga cttcagaggc
      121 ctgacctccg ataacgccgt ctccggggag tttgcgtgat tgctggtgga aaggaaattt
      181 cgcaacagtt accattcgtt cgggtaatgg cccaactttg acttgctagt gtcctgagcg
      241 agaaatccgt ttgcaaagtc gctcgacctt gccgaggatc aaatccgcat cagcggtcca
      301 tacgaagggc ttgggattct cgttgcgctg gtccagatag tcgagcatcg ctttctccaa
      361 cacgcgaacc gcagtgtgac tgcctcgacg gacacagcgc tcggtgacct cggcgaacaa
      421 tcgttccacc agattcagcc aacttccgct ggtcggcgtg aagtgaacgc ggtagcgcgg
      481 gtgacgcgcc aaccaacttc ggaccttggc aaccttgtgc gttccgtagt tgtccatcac
      541 cagatgcacc tccaggccgg cgggaagatt gccctcgatt tcgttgagaa accgcaagaa
      601 ctcctgatgc cggtgccggc ggtagcaatt gctgatggtc actccggagg ctacatccat
      661 agccgcaaac agagaggtaa ctccatgccg ctcgtaatcg tgcgactgcc gagcggggat
      721 acccggcgcc aacggcagaa tcggttcagt ccgattcagc gcctggacct ggctcttttc
      781 atccacgcat agcacgatgg cgcgatccgg cgggttcagg tacaagccca cgatgtcccg
      841 caccttctcc acaaattgcg ggtctttgga gaacttgaag ttctccacac gatgtggctg
      901 taatccgaaa gcacgccaga tccgaacgat ggcgttctgc gacagacccg tttcttcggc
      961 catccggcga gtgctccaat gcgtgctgtt agccggcatc gattccagcg ttttcgtgac
     1021 gacttcttca atttgccgat cactgatctt gcgcggcgcg ccgacccgcg gctcatccag
     1081 caagccatcc atcccaaact ctcgaaaccg ttcccgccat ttaccgacgg tggcgccggt
     1141 gatgtgcagc ttccgcgcga cctcggagtt gctcatcccc tgcccacaac tgagcacgat
     1201 ccgggcgcgc attgccatgg cctgcgccga cttcggccga cgcgcgatca tgctcaattt
     1261 ctcgcgatcc tcatcggcga tttccagagg tttcttgggg cgacctgtgc gcatacccga
     1321 gtatgcggcc agatgtagac ttatgcaacg catttctcgc tcaggacact agttgtgtgt
     1381 gactgccggg ctgtgtgtgc tggcagtctc ttttgcatcc accgcgaaac tgtaaccgaa
     1441 tcctctcaca gatcgaatca tccgggggtt cgctggatca gcctcgatct tttgccgaag
     1501 acggaggatg tagacgtcaa cggtccgatc cgtgaccgct ctatcgtgcc cccacaccgc
     1561 gtcgagcaac tgctcgcggt tgaaaacgac ttcggggcgg cccatcaaaa actcgagcag
     1621 gcggaactca gtcgcggtc
//
LOCUS       DATIFR010000227         1183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2262731, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000227 DATIFR010000000
VERSION     DATIFR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262731"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VLD60_13025"
     CDS             <1..427
                     /locus_tag="VLD60_13025"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSB45930.1"
                     /translation="GNLDCKELTAGSTIYLPVWNEGALFSTGDGHAAQGDGEVDGTAI
                     ETALTGTFEFNVKKKLGWKMPRAETPTHYITFGLDTDLDDAARQALKEMINWIVSMTG
                     IHKDEAYAMCSFAGDLHVTQTVNNVKGVHAMLQKGILKK"
     gene            complement(821..>1183)
                     /locus_tag="VLD60_13030"
     CDS             complement(821..>1183)
                     /locus_tag="VLD60_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45931.1"
                     /translation="MASYARNELGFKTEMTYSLLAGDVTNRWDWQGGRVQASAEDDLR
                     VLLAFGPSFRLLIAHGYSDMITPYAMTRYVIDHLPAFDPSGRVQLKLYRGGHMLYLDP
                     DSRKAFSTDAAMFFRDKE"
BASE COUNT          272 a          328 c          348 g          235 t
ORIGIN      
        1 cggcaatctc gactgcaagg aactgacggc gggcagcacg atctatcttc cggtgtggaa
       61 cgagggtgcg ctgttctcga ccggcgatgg gcatgcggcg cagggcgatg gtgaggtcga
      121 cggaaccgcg atcgagaccg cactcaccgg cacgttcgag ttcaatgtga aaaaaaagct
      181 cggctggaag atgccacgcg cggagacccc gacccactac atcacgttcg gtcttgacac
      241 agacctcgac gatgccgcgc gtcaggcgct gaaggaaatg atcaactgga ttgtcagcat
      301 gaccggcatc cacaaggacg aggcctatgc catgtgtagc ttcgccggcg atctgcacgt
      361 gacgcagacg gtcaacaacg tcaaaggcgt gcatgcgatg cttcagaagg gcattctgaa
      421 gaaatagcta cgcggcggcg tcggccctga ccggtgccga ttcgttattc tcggaccagc
      481 gttctggcat catgggggca tcaatcgctt caattcacga agatggcctg cgcgcgatcg
      541 cgattcttac ggttgttggg tcgagaacgc acaatgacgg tccctcgtca ggcgaccggg
      601 cctttcgaaa acgtcagact catcgatcca atcaatctct tctgtacgga atgccgtccc
      661 aacaagggta ccgggctttt tttctttgac acgagccatc taagctcggc tggagccgag
      721 cacctctacc aaggatacaa gcgtgatttt ttatgagcgc tcactggcga tggtgtcggg
      781 aataaggtct ctaaggactc ctcccgctga cgggagaaac ctactcttta tcccggaaaa
      841 acattgccgc gtcggtgctg aaagctttgc gggagtccgg gtccagatag agcatgtgcc
      901 cgccgcgata gagcttgagc tgcacgcggc cggaaggatc gaaagccggg agatgatcaa
      961 tcacatagcg cgtcatggca tagggcgtga tcatgtcgga atagccatgc gcaattagca
     1021 gacgaaacga tggcccgaac gccaaaagca cgcgtaaatc atcttcggcg ctcgcttgca
     1081 cgcggccgcc ctgccagtcc caacgattgg tcacatcgcc ggccagaagc gagtatgtca
     1141 tttcggtttt gaaaccgagt tcgttgcgag catagctggc cat
//
LOCUS       DATIFR010000228         3494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2457435, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000228 DATIFR010000000
VERSION     DATIFR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457435"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VLD60_13035"
     CDS             complement(<1..1019)
                     /locus_tag="VLD60_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45932.1"
                     /translation="MMKAHLEDTHRSGWFRPLVAVAVLLSLTGCMSFGAMTLDRDRLD
                     FTQAVANSWKQQTLLNIVKLRYGDTPIFVDVGQIVSGYQLVSTVQANGTIFPSSSTPN
                     FFNLGAAGQYTDRPTITYVPLTGSQFIRTLMTPIPPIRLFELVEAGWPIDLLFNVCVQ
                     SVNALSNARAGGQARPADPDYAKLLKAMRRVQASGAVGLRAEPEQDKKGGGLVLFFPV
                     KTIPPEIQAERDTIRRLLGLKPEETEFRIIYGTAHEGANVVAIHTRSGMQILLELSSF
                     VQVPEEHQREQRVFPQAMVPPDGQEAPPPLIRIVSGTTKPAEAFAIIRYNDLWYWIDN
                     RDLRS"
     gene            complement(1043..2299)
                     /gene="pfp"
                     /locus_tag="VLD60_13040"
     CDS             complement(1043..2299)
                     /gene="pfp"
                     /locus_tag="VLD60_13040"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HSB45933.1"
                     /translation="MTETVAILVGGGPAPGINGVISAATIRCLAEGRKVLGIEQGFKW
                     IMQGDVSKVTPLTLEQVSRLHFQGGSVLKTARANPTKNSAHLSQALKSLAQLGVTGLI
                     TIGGDDTCFSALTLERAAQGRLRVVHVPKTIDNDLDLPQGVPTFGFQTARHLGTYAVK
                     SILEDARTTSHWFFVVAMGRKAGHLALGIGKAAGATLTLIPEEFAERPVRLAKVADIL
                     AGAIIKRLSQGREDGVAVLAEGVAEVMNPDDFTALASVSRDEHGHVELADLDLGRALK
                     EEVLKRLAPYGLAPTIRLKDLGFELRCADPIPFDMEYSRDLGCCAAEFLISGGTGAMV
                     SIVNGKFSPMPFDTILDPATKRTRVRMVDVAAEPYRIARRYMIRLDQKDFEQSERLAS
                     CAAVVGLTAEAFRERFASVATHDRGL"
     gene            2844..>3494
                     /locus_tag="VLD60_13045"
     CDS             2844..>3494
                     /locus_tag="VLD60_13045"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSB45934.1"
                     /translation="MGYHELEQTARAMVAEGKGILAVDETVPTLTKRFDALKIVSTED
                     SRRNYREMFFTTPGAAAFISGAILYDETIRQKSSSGTPLADVLAKQGILPGIKVDTGA
                     KTLTGSPDEKMTEGLDGLRERLKEYRELGARFAKWRAVIRITDKLPSPMCVNVNAHAL
                     ARYAALCQEQRIVPIVEPEVLMDGPHTIERCEDVTSSMLHAVFHALFEQCVQLEGML"
BASE COUNT          745 a          992 c         1035 g          722 t
ORIGIN      
        1 ttggagcgaa gatcacggtt atcgatccaa taccacaagt cgttgtatcg aataatcgcg
       61 aaggcttcag ccggcttggt cgttccgctc acaatccgaa tcaggggcgg gggagcctcc
      121 tgcccgtcgg gcgggaccat cgcctgcgga aacacgcgct gctccctctg gtgctcttca
      181 ggaacttgga cgaacgatga aagctcgagg aggatctgca ttcccgagcg ggtatgaatg
      241 gccacgacgt tagccccctc gtgcgccgtc ccgtagatga tccgaaattc tgtctcctcc
      301 ggcttcaacc cgagtagacg tctgatcgtg tcccgctcgg cctggatttc cggagggatg
      361 gttttgacgg ggaaaaagag gacgaggcct ccacctttct tgtcttgctc gggctcggca
      421 cgcaatccga cggcaccgga ggcctggact cgccgcatgg ccttgagcag cttggcgtaa
      481 tcgggatccg cgggtcgcgc ttgtcctccg gctctggcgt tggagagcgc attcacactc
      541 tgcacgcaca cgttgaacag cagatcgatc ggccagcccg cttcgaccag ctcgaacagc
      601 cggattggcg gaataggagt cattagcgtg cggatgaact gagatccggt caggggcacg
      661 taggtgatgg tgggccgatc cgtgtactgg ccagccgctc cgagattgaa gaagttggga
      721 gttgagcttg aagggaaaat ggtcccgttg gcctgcacgg tgctgaccaa ctggtaaccg
      781 ctcacgatct ggccgacatc cacaaaaatc ggcgtgtccc catagcggag cttgacgata
      841 ttgagcaggg tttgttgctt ccaggaatta gccaccgcct gtgtgaagtc caggcgatca
      901 cggtcgagtg tcatggctcc gaagctcatg cagccggtca ggctgagcag gacggccact
      961 gcaaccaatg gccggaacca tccggaccga tgtgtgtctt cgaggtgtgc tttcatcatc
     1021 cagtctcctt agccgggcgg aattatagac ctcggtcatg tgtggccacc gaggcaaatc
     1081 tttccctgaa cgcctcggct gtgagaccga ccactgcagc acagctggcg agtcgctcgc
     1141 tctgttcaaa atctttctgg tccaggcgga tcatatagcg gcgggcgatc cgatagggct
     1201 cggctgcgac atccaccatg cgaacccgcg tccgttttgt ggccggatcg agaatggtgt
     1261 cgaatggcat cggggaaaat tttccattga cgatcgagac catcgccccc gtgcctcccg
     1321 aaatcaagaa ttcggcggca cagcagccca aatcccggga atattccata tcgaacggta
     1381 tgggatctgc acaccgcaat tcgaatccga gatccttgag ccggatagtc ggagcgagac
     1441 catagggcgc cagccgcttg aggacctctt ccttcagtgc tcggcccagg tcgaggtcgg
     1501 ccaattccac atggccgtgt tcgtcgcgag agacagacgc gagagccgtg aaatcatcag
     1561 gattcatgac ttctgcgaca ccctctgcga gaacggccac tccgtcctct cgtccctggc
     1621 tcagccgttt gataatcgcg cccgcaagaa tgtcggcgac cttcgccagt cggactggtc
     1681 gctcagcgaa ctcctccgga atcagtgtga gcgtggctcc ggccgctttg ccgatgccca
     1741 gtgcgaggtg gccggctttg cgtcccatgg ccacgacaaa aaaccaatgc gaagtcgtgc
     1801 gtgcatcctc gagtatgctc ttgaccgcat aggttccaag gtgcctggcg gtctgaaatc
     1861 caaaagtcgg taccccttga ggaagatcga ggtcgttatc gatggtcttg gggacgtgga
     1921 cgacgcgcag gcgtccttgc gcggcgcgtt cgagtgtcag cgcggaaaag caagtgtcat
     1981 ccccgccgat cgtgatcagt cccgtgacgc cgagttgcgc gaggctcttc agcgcttggg
     2041 aaagatgcgc ggaattcttg gtggggttgg ctcgcgcggt tttgagcacc gatcctcctt
     2101 gaaaatgaag tcggctgact tgttcaaggg tcagcggcgt gaccttgctc acgtcacctt
     2161 gcatgatcca cttgaaacct tgttcgatgc ctaagacctt tctgccctcc gcgagacatc
     2221 ggatcgtcgc ggcgctgatc actccgttga tacctggtgc aggacctcca ccgaccagta
     2281 tggcgactgt ttccgtcatt gctgtccctc cttcacgggc cgatggcacc gtcctcgtta
     2341 cacaaaatga gatgcgttgt tccgcctctt tttatgtgcc aggaacatgc cagcaagcca
     2401 ctcgacagct aaacgaaggc agtccaagtt tttgttgtgg catgcatagt cagagagaga
     2461 aaaaagccag ttctttgaat aggctcgcag aaagaggcag ggtcccggca gcaaaccaca
     2521 gcgaaggcat ggcagttcca gctgaggaaa tttgggacta ttccattcct ccacctggcc
     2581 cgaatatcta catagaaccc ctgtcgaccg gcatcggatg atcccaaaat ctcagaaatc
     2641 ttgcacagca gtggtcctgt gatcggacat tcataatggt acgtagattg ctttgctcat
     2701 gtgtgatcgc tgccgcaact gagaggacta tgttttgcaa gctgaatttc tgcggagacg
     2761 tcggatgagc cgggcggaac aggatggggt cggactgatc acagtcgaaa cgacggaacg
     2821 ctgaagacgg aggaggaaca agtatgggtt accacgaact ggaacagacg gcaagggcga
     2881 tggttgccga gggaaaagga atcctggcag ttgacgagac ggtgccgacg ctgaccaaac
     2941 gattcgatgc cctgaagatt gtctccactg aggacagccg ccgtaattat cgcgaaatgt
     3001 tttttacgac gcccggtgct gctgccttca tcagcggtgc gatcttgtat gacgagacca
     3061 tccgccagaa gagctcgagt ggaacgccac tcgccgacgt gctggccaag cagggcattc
     3121 tgcctggcat caaggtcgac acgggagcga aaacgctgac cggatccccg gacgaaaaga
     3181 tgaccgaagg cctggacggc ttgcgggagc gactcaaaga atatcgggag ctcggcgccc
     3241 gcttcgcgaa gtggcgggcc gtcattcgca tcaccgacaa actacccagc ccaatgtgtg
     3301 tgaacgtcaa cgcccacgcg ctggcgaggt acgccgccct gtgtcaggag caacgcattg
     3361 tgccgatcgt ggagcccgaa gttctgatgg atgggcctca caccatcgaa cgctgcgagg
     3421 acgtcaccag ttccatgctc catgcggtgt tccacgcgct cttcgaacag tgcgtccagc
     3481 tggaaggcat gctc
//
LOCUS       DATIFR010000229         6082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2077508, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000229 DATIFR010000000
VERSION     DATIFR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077508"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VLD60_13050"
     CDS             complement(<1..610)
                     /locus_tag="VLD60_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSB45935.1"
                     /translation="MAKTLFDKVWEAHVIMTTPDETSLIYIDRQLVHEVTTAQAFEGL
                     RLTGRKVRRPELTFATMDHNIPTDDPFNIKDSIAREQVDALTKNCRDFGITLYDLRNE
                     KRGIVHVIGPELGLTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLRQK
                     KAKSYKVDFVGTRTKGVTAKDLILKLIGLIGTAGGTGHVFEYM"
     gene            complement(711..1580)
                     /locus_tag="VLD60_13055"
     CDS             complement(711..1580)
                     /locus_tag="VLD60_13055"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HSB45936.1"
                     /translation="MKTIFGIALLVLASVPALAENANTEWKAVESILGREGVIQEGVL
                     KVLFPRADLEIRMKGSRVQADFALSSWLGFKKVGSQTMLMGDFVLLDEEVAPVLEKLV
                     ASGLEVSALHNHFVGESPTLKFMHFMGHGRAIELAKKMRTLLQATGTPLDAPPAVLVS
                     EIPQWKVIESIFGLTGRRKGDLLLFSIPRRETIREHGVEIPAIMGMASAIHFQAAGRD
                     VVSAGDLTLLPEEVNPVVSELIKNGVDVTAIHNHTIFEEPRLFSVHFWAQGKAEKVAK
                     AVKKALSRTNSRL"
     gene            complement(1613..2386)
                     /locus_tag="VLD60_13060"
     CDS             complement(1613..2386)
                     /locus_tag="VLD60_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB45937.1"
                     /translation="MSKQAASERFNKIASNFVTSEVHANSPTIQKLHGLLDLPANATV
                     CDVACGAGHLALSFGGKASRIVGVDPAPNMLSAFQGLAESKGIRVEAFRSGAENLPFE
                     DGSFDLVMSRLAPHHFRDIQKAVREMARIAKKGGYVAVIDLEGHPDPGIDEFNHRLEV
                     LHDPTHGRSYTASHWRELFERAGLRILTLENELSEKPGGVPIRRWCEIASSGAEAELE
                     IRRLVREAPPRVREELGITQNGDEFHMPVRTVLILAQKP"
     gene            complement(2409..3872)
                     /locus_tag="VLD60_13065"
     CDS             complement(2409..3872)
                     /locus_tag="VLD60_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB45938.1"
                     /translation="MLSIKEPRHSGIELNLGALLDRHPELQINQEEYNINVTANYGES
                     LTRDEVLEKFAQHDGAGRPAHLYLHVPLCSYICHFCNYVKKALPKNGREEETLEAWVS
                     RLIEESKRYLERFDWIGKARIESIYLGGGTAALMRESQLGRLLAHIHERYNLSHVQEV
                     TLEGNPDNYDRDFVRAIQRLGFNRFSVGVQSFQDRVTAFTGRGHDRLASLKAIKVLLD
                     SGDPFNVDMMFGLPYQTPGTVEEDLRTLVELGVPTITIYRLRNAERHKMGIGNVSVWN
                     VPSVRDKLYKQGLFPSLEETYAMREAAVRVLLEFGYQPSPCGWWSRPGTYPGGNIPHV
                     SANKWQHYHTMLAVGPGAYGWLTGRRGEVIQTHNETDIAAYVRHLESQSTPPLSFGRH
                     LQGHQAVASALGFAFKANQPILFDRFADEYRVDLLREEPYRSVIEDLLEKRLVEVAPT
                     GRSIRPTLEGEALHEEIISVYIHGRIGSFQDAVCKRF"
     gene            complement(3909..4535)
                     /locus_tag="VLD60_13070"
     CDS             complement(3909..4535)
                     /locus_tag="VLD60_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45939.1"
                     /translation="MKKSIVAAVIAAPLLTALVVTSASSQSQDNVFRRFVTSKPAVEN
                     AAVTASFASSVNRFFPAANPKVAAVFKEAVESPEAFSKFTFREDTPFTVTVEGSLGRQ
                     NIQSTFRDFQEALFTVVPKEQHVFVASTLLGIASAFKDSCTFFRFGPDGSIVHVGIEG
                     TAYVPGEISQVPRGSVTVSPMTVDVAKRFATALRGKSVNVEYTIDGIE"
     gene            4837..5619
                     /locus_tag="VLD60_13075"
     CDS             4837..5619
                     /locus_tag="VLD60_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB45940.1"
                     /translation="MSNASVPVWNPSQYLGFADYRLRPAIDLISQMSVERPGTIYDLG
                     CGAGNVTKVLQERWPLARIFGVDNSAEMLKVARAKVVGVEFLDKDLFNWAPPEPADIL
                     FSNAALHWLGDHETLFPRLVSWLKPGGTLAIQMPRNHMAPSHTLISDSAGAGPWREKL
                     TPIPGPRNVGAPEDYYSLLAPLLGGLRIWETEYLQVLTGENPVVEWTKGTTLVPILSL
                     LDEPDRSAFLADYSARIRRAYPPCADGKTLFPFRRLFIVGSH"
     gene            complement(5671..>6082)
                     /locus_tag="VLD60_13080"
     CDS             complement(5671..>6082)
                     /locus_tag="VLD60_13080"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB45941.1"
                     /translation="NGYRCCALSLVRLVLGVIFFMHGGQKVMGWFGGQGLAATVQMMS
                     AHLPVVLVYLVAFGEFLGGIALIVGFLSRLAAMGIFIIMAGAIATVHWKNGFFAPQGF
                     EYPLALAAMALAVIVGGSGCLAIDSFWCRKKTAA"
BASE COUNT         1304 a         1701 c         1725 g         1352 t
ORIGIN      
        1 ccatgtactc aaagacgtgt cccgtcccgc cggcggtgcc gatcagcccg atcagcttca
       61 ggatgaggtc cttcgccgtg acgcccttgg tgcgggtccc gacaaagtcg accttgtacg
      121 acttggcctt tttttggcgg aggcattgcg tcgccaagac gtgttcgacc tccgaggtgc
      181 cgatgccgaa ggccaaggcg ccgaaggccc cgtgggtcga cgtgtggctg tcaccgcaga
      241 caatcgtcgt tccgggaagg gtgagtccca attccgggcc gatgacgtgc acgatgcccc
      301 gcttctcgtt tctcaaatca tagagagtga ttccaaaatc acggcaattc ttcgtgaggg
      361 cgtcaacctg ctccctggca atcgaatcct tgatgttgaa aggatcgtcc gtcgggatgt
      421 tgtgatccat ggtggcaaag gtaagttccg gtcgcctcac ttttcgtccg gtcaaacgga
      481 gtccctcgaa ggcttgggcc gtcgtgactt cgtgaacgag ttgccggtcg atataaataa
      541 gggaggtctc gtcgggcgtc gtcataatga catgggcttc ccacactttg tcaaaaaggg
      601 tctttgccac gtttcagcac ctcaaataat caatccctga atcgcaccat ttggtgtgaa
      661 agttcatcct aaatgaggcg aatcggcatt ttcaacggaa agttgcttca ttagaggcgc
      721 gaattggtcc ggctcaaggc cttcttcacg gctttggcca ccttttcggc ttttccttgg
      781 gcccaaaaat ggacggaaaa caatcgaggc tcctcgaata tcgtatggtt atggatggcg
      841 gtcacgtcga cgccgttctt gataagctca gaaacgaccg gatttacctc ttcgggaaga
      901 agggtgaggt ctcccgccga cacgacgtcc cggccagcag cttggaaatg gatggcggat
      961 gccatgccca taatggcggg aatttccacg ccgtgctcgc gaatcgtttc gcgcctgggg
     1021 atggagaaaa gaagcaagtc tcctttgcgg cggcccgtaa ggccaaaaat ggattcaatg
     1081 accttccatt gcggaatctc cgaaaccaag accgcgggag gtgcatcaag gggcgtgccc
     1141 gtcgcttgaa gcagcgtcct catctttttg gcgagttcaa ttgctcttcc atgacccatg
     1201 aaatgcatga acttgagagt gggactttcg cccacgaagt ggttgtgaag ggccgacacc
     1261 tcaagtccgc tggcgacgag cttttcgaga acgggagcga cttcctcatc caacagaacg
     1321 aaatccccca tcagcatggt ttggctcccg accttcttga accccagcca ggacgaaagc
     1381 gcaaagtccg cctgaacgcg gctccccttc attcggattt ccaggtcggc gcggggaaat
     1441 agaaccttga gcacgccttc ctggattacg ccttcgcggc ctaaaatact ttcaaccgcc
     1501 ttccattcgg tgttcgcatt ttctgcaagc gccgggaccg aggctaggac aagcaaggca
     1561 atgccgaaga ttgttttcat gggtgtgtca taccgacgtt ttaaaccgct gtctaaggct
     1621 tttgggcgag aataagaacc gtccgaacgg gcatgtgaaa ctcatcgccg ttctgggtaa
     1681 tgccgagctc ctcgcggact cgaggcgggg cttccctcac gaggcggcgg atctccagct
     1741 cggcctcggc acctgacgag gcgatctcgc accagcgccg gatgggaaca ccgcccggtt
     1801 tttccgagag ctcattttca agcgtcagga tgcgaagacc tgcccgctcg aagagttccc
     1861 gccaatgcga cgcggtataa ctgcggccgt gtgtcggatc gtgcaggact tcgagacggt
     1921 gattgaattc gtcgatcccc ggatccggat gaccctccaa atcgatgacg gcgacgtatc
     1981 cgcctttctt ggcgattctg gccatctcgc ggacagcctt ctgaatatcg cggaaatgat
     2041 gaggagccag cctggacatg accagatcga atgagccgtc ttcaaaaggg aggttctccg
     2101 cgccggatcg aaaggcctcg acgcggatgc ccttgctttc cgccagtccc tgaaaagctg
     2161 acagcatgtt gggcgccgga tccaccccca cgattctcga tgccttgcct ccgaatgaaa
     2221 gggcaagatg gccggccccg cacgcgacgt cacagacggt ggcgtttgcc ggcaagtcga
     2281 ggaggccgtg cagcttctgg attgtggggc tgttggcgtg cacctcgctc gtcacgaaat
     2341 tggaggcgat tttattgaag cgctcgcttg ccgcttgttt gctcatagaa aggtctccgc
     2401 agggtttttt agaatctctt gcacacggca tcttggaacg atccgatgcg cccatgaata
     2461 tagacggaaa tgatctcctc gtgaagggcc tctccttcca aggtcggcct gatcgaacgg
     2521 cccgttggcg ctacctctac aagtctcttt tccagaagat cctcgatcac gctccggtag
     2581 ggctcttccc ggaggaggtc gacgcgatac tcgtccgcga agcggtcgaa caagatcggc
     2641 tggttcgcct tgaaagcgaa gcccagcgcg gaggcgactg cctgatggcc ctggagatga
     2701 cgcccgaacg acaaaggcgg cgtgctttgg ctctccaagt gccggacata ggcggcgata
     2761 tccgtttcat tgtgggtttg aataacctct ccacggcgtc ccgtcagcca gccataggcc
     2821 cctggcccaa cggccagcat cgtgtgatag tgctgccatt tgttggcgga aacgtgaggg
     2881 atatttccgc cggggtaggt cccgggcctg ctccaccaac cgcaagggct gggttggtaa
     2941 ccaaattcga gaaggacacg aacggcggcc tctctcatcg cgtaggtttc ttccagggat
     3001 gggaagagtc cctgtttata gagtttgtcc ctgacgctgg gtacgttcca cacggacacg
     3061 ttgccgattc ccattttgtg tctctcggcg ttgcggaggc ggtagatagt gatggtgggc
     3121 actcccaatt caacgagcgt acgaagatcc tcctcaacgg ttccaggagt ctggtaaggc
     3181 aacccgaaca tcatgtcgac gttgaaggga tcgccactgt ccaggaggac cttgatggcc
     3241 tttagagagg caagccggtc atgccccctt cccgtgaagg cggttacccg atcctggaaa
     3301 gactgtacgc ccacgctgaa ccggttgaaa cccaatctct gaatcgcacg gacgaagtcc
     3361 ctgtcgtaat tgtcggggtt cccctccaac gtgacctctt ggacgtggct gagattgtag
     3421 cgttcgtgga tatgcgccag aaggcgcccc aattggcttt cccgcatcag cgccgccgtc
     3481 cctccgccca agtagatgga ttcaatacgc gccttgccga tccaatcgaa acgctccaga
     3541 tacctttttg actcctcgat aagccttgag acccaagcct ccagggtctc ctcttccctt
     3601 ccatttttcg gcaacgcctt tttgacgtag ttgcagaagt gacagatgta ggaacagaga
     3661 gggacatgga gatagagatg ggccggacgt cctgcgccgt catgctgggc gaacttttcc
     3721 aggacttcat cccgggtgag ggactcgccg taattggcgg ttacgttgat gttgtattcc
     3781 tcctgattga tctggagctc gggatggcgg tcgagcaagg cacccaggtt gagttcaata
     3841 cctgagtgcc tcggctcttt gattgaaagc atgtcgcaag acatcatgga tggcatcgac
     3901 ccatcttttt attcgatgcc gtcgattgtg tattccacgt tcaccgactt gccgcgaagg
     3961 gccgttgcga accgtttggc aacgtcgacg gtcatcgggc ttacagtcac ggaaccacgc
     4021 ggcacttgac tgatttcgcc gggcacataa gccgtgcctt caatcccgac atggacaatg
     4081 ctgccgtccg gcccgaacct aaaaaaggtg cacgaatcct tgaaggcgct tgcaataccg
     4141 agcaaggtgg aggcgacaaa cacgtgctgc tcttttggaa cgaccgtgaa tagtgcctcc
     4201 tgaaagtctc taaacgtcga ttggatattt tgacggccaa gggatccttc aacggttact
     4261 gtaaatggcg tatcctcacg gaaggtgaac ttggagaatg cttcagggct ttcaacggcc
     4321 tccttgaaga cggccgcgac tttgggatta gccgcaggaa agaagcgatt taccgagctc
     4381 gcaaaactcg ccgtgaccgc ggcattctcg acggccggtt ttgaggtcac aaaccttcga
     4441 aagacgttgt cctgcgactg gctgctcgcc gacgtgacga ccaaggcggt caaaaggggc
     4501 gccgcaatga cggccgccac aatgctcttt ttcatgatct cctcctatgg attggtttgg
     4561 tggaatgatc ggatcacccg tccgtcactc aggtgggtct ctcattgtga agtgataggc
     4621 atgcaatcgt aacgccatga agcggtgcgt tgtttcttat gatgtaagcc actgatattg
     4681 ttcgatgtca tttgactctt gttgttgagc ggcggaaaaa aagggggcaa aagatacccc
     4741 cagacggctc ttcttatcag gatgagaatg aatcatttga actattgagg ttttttcggc
     4801 acaactctct gaaggtcgga gtcgagaaaa gcgatcatgt caaacgcgtc cgtcccagtc
     4861 tggaatccct ctcaatatct cggcttcgcc gattatcgcc tcaggccggc gattgatctg
     4921 atctcgcaga tgtccgtcga aaggccgggt acgatttacg acctcggttg cggggcggga
     4981 aacgtcacca aagtcttgca agagagatgg ccgctggcgc ggatatttgg agtcgataat
     5041 tcagcggaga tgctgaaagt cgcccgcgcc aaagtggtgg gtgttgaatt tcttgataaa
     5101 gatctcttta attgggctcc tccggaaccg gccgacattt tattttccaa tgccgccctc
     5161 cactggttgg gtgaccatga aacgctcttt ccgcgcctcg tttcctggct caaaccgggc
     5221 gggacgctcg ccatccaaat gccgcgaaat catatggcgc cgtcccatac cttgatttcg
     5281 gacagcgccg gggcagggcc atggagggaa aaactcacgc cgattccggg tccgaggaac
     5341 gtcggagctc cagaagatta ttattccttg ctagcacccc tgttgggggg gcttaggata
     5401 tgggaaacgg aatatcttca ggtgctcaca ggggaaaacc cagtcgttga atggaccaaa
     5461 gggacgacgc tggtcccgat cttgtcactc ctcgacgaac cggatcgaag cgcattcctg
     5521 gccgattatt cagcccgaat caggcgcgcc tatcctccgt gcgccgacgg aaagaccctc
     5581 tttccttttc gcagattatt catcgtcggc tcgcactagc ttccattgtg cgctgaagcg
     5641 cgagttgccc cgctggaatc gggaccagaa tcaggcggcg gtcttcttcc ggcaccagaa
     5701 gctgtcgatc gcgaggcacc cgcttccgcc cacgatgacg gccagggcca tggccgccaa
     5761 ggccaaggga tattcaaatc cctggggcgc gaagaatccg ttcttccagt gcaccgtggc
     5821 gatcgctccc gccatgatga tgaagatgcc catagccgcg agtcttgaga ggaatccgac
     5881 gatgagtgcg atgccgccca ggaattcgcc gaaggcgacc agatagacga ggaccaccgg
     5941 gagatgggcg gacatcattt ggaccgtggc cgccaaaccc tgaccgccga accagcccat
     6001 caccttttgg ccgccgtgca tgaagaagat gacgcccaac acaaggcgga caagggacaa
     6061 ggcgcaacac cggtagccgt ta
//
LOCUS       DATIFR010000230         1025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2458373, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000230 DATIFR010000000
VERSION     DATIFR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458373"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /locus_tag="VLD60_13085"
     CDS             complement(<1..>1025)
                     /locus_tag="VLD60_13085"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSB45942.1"
                     /translation="STAQRRVYIMLVVAIFLAAAFMYLTGEWILRPITRLTRSADEIR
                     QGNLDLVVQSSSRDEIGQLSEAFNAMAASLREFRRSDQAKLLRIQRSTQQALNSLPDV
                     IAVLDSDGVVEVSTEAAREVFGLKPAVHLQNLPYPWMSDLFEQSLRTGRITESAEATK
                     VVQQFVRGEERFFHPKALPIMDSDKLAAGIVLVLSDVTQQFHQEELKRGVISTVAHQL
                     KTPLTSIRMALHLLLEDKVGALTEKQAELIVAAREDADRLNTILKDLLDIGRIESGKA
                     PMEFRPVFPHTMVSDAVYLYRAAARDGGVSLIVDLPENLPRVWVDSKQIAHVFANLLS
                     NALKYTLP"
BASE COUNT          209 a          303 c          308 g          205 t
ORIGIN      
        1 gggagggtat acttcagcgc gttcgatagc aggttggcga aaacgtgggc gatctgcttc
       61 gaatccaccc acaccctcgg cagattttcc ggcagatcga cgataaggga cactccacca
      121 tcacgagccg ccgcgcgata caggtacact gcatccgaaa ccatggtgtg cggaaaaacg
      181 ggacgaaatt ccatcggggc cttgccggat tcgatccggc cgatgtcgag gagatccttg
      241 aggatcgtgt ttaaccggtc ggcatcttcc cgggcggcca cgatgagctc cgcctgcttt
      301 tccgtgaggg ctccgacctt atcctcgaga agcagatgca acgccatgcg aatggaagtc
      361 agcggggttt tcagctggtg ggcaaccgtt gagatgaccc ctcttttcaa ctcttcctgg
      421 tggaactgct gggtaacatc actcaagacc aggacgattc ctgccgccag cttgtcggaa
      481 tccatgatcg gcaaggcctt gggatggaag aaccgttcct cgccacgaac gaattgctgg
      541 accaccttgg tcgcctcggc ggattccgtg atgcgaccgg tccgcaggga ctgttcgaac
      601 aagtccgaca tccaggggta cggaaggttc tggagatgca cggcaggctt caacccgaac
      661 acctccctgg ccgcttcggt cgatacttcg accacgccgt cggaatccag gacggcgatc
      721 acatcgggga gactgttgag cgcctgctgg gtggatcgct ggattcgaag cagctttgcc
      781 tggtcgctgc gccggaattc ccgcaaggac gccgccatgg cgttgaatgc ctctgaaagc
      841 tgtccgatct cgtctcggga gctgctctgg acgacgaggt caagattccc ttggcggatt
      901 tcgtccgcgg atcgagtcag ccgggtgatc ggccgaagga tccattcccc cgtcaggtac
      961 atgaaggccg cggccaggaa tatcgccact accagcatga tgtacacccg acgctgcgcc
     1021 gtcga
//
LOCUS       DATIFR010000231         8680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1501241, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000231 DATIFR010000000
VERSION     DATIFR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501241"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VLD60_13090"
     CDS             <1..419
                     /locus_tag="VLD60_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSB45943.1"
                     /translation="LKLAMKARDQLRMDVIRMIKAAVLNKEVEMKKDLDDAEMSRIMT
                     TMIKQRKESVEQYEKGHRAELAAKERQEILIIESYLPKALSTDELAQIVEAVIRETGA
                     SSAKDMGATMKAVMARLAGQPVDGKQVSDLVRSKLK"
     gene            496..1134
                     /locus_tag="VLD60_13095"
     CDS             496..1134
                     /locus_tag="VLD60_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45944.1"
                     /translation="MSILIVDDSPDQQALLRSILGKAGHEAIVSADSAKSASAVLNLE
                     GDAPTQKIDLILMDVLMPEQDGVETCRQIKRCAHLQDIPIIMVTAKSDLSNLKAAFSA
                     GAMDYISKPVNSVELLARVSSALTLKSEMDRRKTRELELRRSNEELQHALKEVKVLRG
                     LIPICASCKKIRNDGGFWQQLEEYLGEHSEAEFSHGLCQPCIKKLYPGVYQD"
     gene            1184..2188
                     /locus_tag="VLD60_13100"
     CDS             1184..2188
                     /locus_tag="VLD60_13100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSB45945.1"
                     /translation="MSILIVDDSPDDRTLLQSVLTAAGYEEILVAASAAEAFRILGLD
                     GGKQGAARVDLILMDIIMPEMNGIEACRQIKAVERFRETPIMMVTVKTDPVDLQLAFA
                     AGALDYIGKPVNKIELLTRVRSVLRLVHEIDRRRAREQELLEVMRQLQEANQMLLRLS
                     CLDGLTGITNRRQFDDFLDQEWRRAVRESTPLSLIMLDIDRFKIYNDTHGHTAGDECL
                     RQVANAISGAVNRPGDLVARYGGDEFVVVLPGTTTEGAAQVAETLRRRIEALGIMHAE
                     GDNMTISVGFASTIPNRAASPTDLIKAADQALYQAKQEGRNRAKQAAILTLVPHSEDE
                     "
     gene            2224..3963
                     /locus_tag="VLD60_13105"
     CDS             2224..3963
                     /locus_tag="VLD60_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45946.1"
                     /translation="MPRSHRPPRHKTARKSRHPALAQVQSALQRERAARKREAVERRD
                     AEQALLIAKEAAEVASRAKSEFLASVSHEIRTPMNAIIGMADLLWETNLTPDQRKYLR
                     IFRRAGSNLLHLINDILDLSKVEAGHIELESIEFDLSDLIEKAIEILAMRANEKGLEL
                     ACHLSPDVPCALIGDPNRLHQILVNLISNAIKFTDKGSVVVEIMNDPDHPSPGAIRFS
                     VTDTGIGIPPNKLGAIFESFTQAHASITRQFGGTGLGLTISRQLAELMHGKIWVESSE
                     GKGSTFHCRVQLSVKPRETAAPSGITLDLQRIRTLVVDDHPTNRQILTESLAGWNADI
                     MDAPNGPDALKELRREATSGQPYRLLLLDCRMPGMHGFQVVEEIQRSGLSKDLTIVML
                     ASDHWADDIARTYDMGLGGYLTKPIRRSDLLQTISIALDRTRGVPSAQPHAAPSVPAS
                     SRPLHILLVEDSPDNQVLVRSYLKQTSHQLDVADHGGIAVERFKKGQYDLILMDMNMP
                     VMDGYEATKQIRAWELDHDLPPTHIIALTALALKEEGEKILEAGCNAHMTKPVKRQTL
                     LEVLHACTGHRKT"
     gene            3960..4286
                     /locus_tag="VLD60_13110"
     CDS             3960..4286
                     /locus_tag="VLD60_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSB45947.1"
                     /translation="MTPQSANNSTGKVIVTIDQGLEEIVPGFLENRRKDVASLETAMQ
                     KNDLKTVRLLGHRMKGDGGGYGFQEISAIGDVLEHAAMREDRTVITEQINKLADFLAR
                     VEIAYR"
     gene            complement(4504..4722)
                     /locus_tag="VLD60_13115"
     CDS             complement(4504..4722)
                     /locus_tag="VLD60_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45948.1"
                     /translation="MCAGCGEPMQFENKSSNQTVPQDEPACNQELSQHVSGLAYAQET
                     MARVDPMQTCMERKGWSRVDHPSLPSNP"
     gene            complement(5124..5456)
                     /locus_tag="VLD60_13120"
     CDS             complement(5124..5456)
                     /locus_tag="VLD60_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45949.1"
                     /translation="MWFLLISFLLLAPAIVQAESRERPMVPPLPADSGPITVIPWGTG
                     TTSYWDDKGQETRTYQTFPGIGYYTGPAGSGFMYDNFSRRELSVPSTSPSAPWPGTEN
                     DLDCRSCR"
     gene            complement(5840..6262)
                     /locus_tag="VLD60_13125"
     CDS             complement(5840..6262)
                     /locus_tag="VLD60_13125"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HSB45950.1"
                     /translation="MSRRVFSRVRMVALVAGVCLLSANLEITPVAAAPDAPQGPFAGV
                     TQNWDKNLPSASRFTVLTVFGGAAVRDNNTGLVWEQAPSADLVIWRIATSNCLNKQVG
                     KTVGWRLPSEAELSSLRDPTLQIPFVPTSVFTGVLDTY"
     gene            complement(6829..7002)
                     /locus_tag="VLD60_13130"
     CDS             complement(6829..7002)
                     /locus_tag="VLD60_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45951.1"
                     /translation="MTLHVGEWSNAVDTRRADRDRPRDQSVLLRTVVLVGYIIASALL
                     IYYWLSPLRSIGL"
     gene            complement(7841..8362)
                     /locus_tag="VLD60_13135"
     CDS             complement(7841..8362)
                     /locus_tag="VLD60_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45952.1"
                     /translation="MQDSNTLKVTRFLLFAVAGFIVAVVCLPLVIGYGTGLRNTLGAV
                     PEYFVKCLPGWSLTSWYEHDELFIPIDNFQRFLDKGCPEIEPRNSPGAAAYISNRVFL
                     FIQRNLDRWYFIGYRAGVDNAIETILPAWNWAFKNHARVRKALLPVLGLILGFIVVGV
                     FAGLRYFLFSRVS"
BASE COUNT         2065 a         2529 c         2373 g         1713 t
ORIGIN      
        1 atctcaagct cgccatgaag gcccgtgacc aactccggat ggacgtcatc cgcatgatca
       61 aggccgccgt actcaacaaa gaagtcgaga tgaaaaagga tctcgacgac gcggaaatga
      121 gccggatcat gaccaccatg atcaagcagc gtaaagaatc ggtggaacag tatgagaaag
      181 gccaccgggc cgaactcgcg gcgaaagaac gtcaagaaat cctgatcatc gaatcctacc
      241 tccccaaggc cctgtccacg gacgaactcg cccagatcgt cgaggcggtg atccgtgaaa
      301 ctggcgccag ctcagccaag gacatgggcg cgaccatgaa agcggtcatg gcccgtctgg
      361 ccggtcagcc ggtggacggc aagcaagtca gcgacctcgt tcgttccaag ctgaaatagc
      421 accatttttc atctgctata ctttatgacg tgcgcgttgc ggcgcatgca ttaaggcaag
      481 acacgtagga tttccatgag catactcatc gttgacgatt caccggacca gcaggcgctc
      541 ctccgctcga ttctgggcaa agccggacac gaggcgattg tcagtgccga ctccgccaag
      601 tccgcctctg ccgtgctcaa tctcgaagga gacgccccga cgcagaagat cgatctcatc
      661 ctcatggatg tgcttatgcc ggagcaggac ggcgtggaaa cctgccgcca gattaagcgc
      721 tgcgcgcatc ttcaagacat tccaatcatc atggtgactg ccaagagcga cctgagcaat
      781 ctcaaagccg ccttttccgc cggcgccatg gattacatca gcaaacccgt caacagcgtc
      841 gaactgctcg cgcgtgtttc gtcggccttg acactgaaga gtgaaatgga ccgccggaaa
      901 actcgcgagc tcgagttgcg tcgcagcaac gaagaattac agcacgccct caaggaagta
      961 aaagtcctgc gcgggctgat tccgatttgc gcctcctgta aaaagatccg caacgacggg
     1021 ggtttctggc aacagctgga agagtatttg ggtgagcatt ccgaggctga attcagccac
     1081 ggcctctgcc aaccctgtat caaaaagctt tatcctggtg tttaccagga ttagctgtta
     1141 ggatttcttc gattttacgg gcctatttga ggcgctaccc aacatgagca tcctcatcgt
     1201 cgacgactcc ccggatgatc gcacactcct tcagtcggtt ctcacggctg cgggttatga
     1261 agagatcctt gtggccgcct ctgccgccga ggcattccgc atcctcggcc tcgatggcgg
     1321 caaacaaggc gcggcccgtg tcgacctgat tctgatggac atcatcatgc cggagatgaa
     1381 cggcattgaa gcctgccggc agattaaagc tgtcgagcga ttccgcgaaa cccccatcat
     1441 gatggtgacg gtcaagactg atccggtcga cttgcagctg gccttcgccg caggcgcgct
     1501 cgattacatc gggaagcccg tcaacaagat tgagctgttg acccgtgtgc ggtcggttct
     1561 gaggttggtg cacgagatcg atcggcggcg ggccagagaa caggaactgc tcgaagtgat
     1621 gcgccagctc caggaagcca accaaatgct gctgcgcctc tcctgtctcg acggtttgac
     1681 cggcattacc aaccggcggc agttcgatga tttcctcgac caggaatggc gccgggccgt
     1741 acgcgaatcg acgccactct cgttgatcat gctggatatc gaccgcttca agatttacaa
     1801 cgacacccac ggccacaccg ctggagacga atgcctaagg caggtcgcca atgctatttc
     1861 gggtgccgtg aaccggccgg gagaccttgt cgcgcgctac ggaggagacg aatttgtcgt
     1921 ggtgcttccc ggaactacca cggaaggtgc ggcgcaggtc gccgaaactc tccggcgacg
     1981 gattgaggcc ctgggcatca tgcatgccga aggcgacaat atgacgatca gcgtaggatt
     2041 cgccagtacg attccgaacc gggctgcttc acccaccgat ttgatcaagg cggcagacca
     2101 agcgctctac caagcgaaac aagaaggccg caatagggcc aagcaggccg ccattctcac
     2161 actcgttccc cactcggaag acgagtaagc gcaattggcc gcgccccttg ctgcgcaccg
     2221 atcatgcctc gatctcacag gccaccccga cacaagaccg cccgaaagtc ccggcacccc
     2281 gccctggctc aggtgcaatc cgccctccaa cgcgaacgtg cggcgcggaa gcgggaggcc
     2341 gtcgagcggc gggatgccga acaggccctt ctcattgcca aagaagcggc tgaagtcgcc
     2401 agccgggcga aaagcgaatt cttggcgagc gtcagccacg aaatccgtac gcccatgaac
     2461 gccatcatcg gcatggctga cctcctctgg gaaacgaatc taacgcccga tcagcgcaag
     2521 tatctgagga ttttcaggcg tgccggcagc aacctgctgc atttgatcaa cgacatcctc
     2581 gacctttcga aagtcgaagc gggtcacatc gaactcgagt cgatcgaatt cgacctgagc
     2641 gacctgatcg agaaggccat cgaaattctg gccatgcggg ccaatgagaa aggcctcgaa
     2701 ctcgcctgcc atttgtctcc cgacgtgcca tgcgccttga tcggagaccc gaaccgcctg
     2761 catcagattc tcgtcaacct catcagcaac gcgatcaagt tcaccgacaa ggggtccgtc
     2821 gtcgtcgaaa tcatgaacga ccccgaccat ccttcccctg gggcgattcg cttctcggta
     2881 acggataccg ggatcggcat tcctccgaac aaactggggg ccattttcga aagtttcacc
     2941 caagctcatg cgtcgatcac gagacaattt ggaggaaccg gactcggatt gacgatctcc
     3001 aggcaactcg cagaactgat gcacggcaag atctgggtgg aaagcagcga gggaaaaggc
     3061 agcacgtttc actgccgggt tcagctcagt gtcaaacccc gtgaaaccgc cgccccgtcc
     3121 ggcatcactc tggaccttca gcgcattcgc acgctcgttg tcgacgatca tccgaccaat
     3181 cgccagatcc tcacggagag cctggccggc tggaacgcag acattatgga tgcgcctaac
     3241 ggtccggacg cgttgaagga gttacgacgc gaagcaacct cggggcagcc ctaccgattg
     3301 ctcctcctcg attgccgcat gcccggcatg cacgggtttc aagtggtcga agagatacaa
     3361 cggtcgggtc tgtcgaagga cctcaccatc gtcatgctgg cttcagacca ttgggcggac
     3421 gatattgccc gcacctacga catgggcctg ggcggctact tgaccaaacc catccggcga
     3481 tccgatctgc ttcaaacgat cagtatcgcg ctcgatcgca cgcgcggcgt tccctcagca
     3541 caacctcatg cggcgccttc cgtgccggca tcgtcgagac cgctgcatat tttgctggtg
     3601 gaggattccc ccgacaacca ggtcttggtt cgttcctacc tcaaacagac cagccatcag
     3661 ctcgatgtgg ctgaccacgg ggggattgcc gtggaacggt tcaagaaggg tcagtacgat
     3721 ttgattctga tggacatgaa catgcccgtc atggacggct atgaggcgac caagcagatc
     3781 cgcgcatggg agcttgacca cgacctgcct ccgacccaca ttatcgccct cacggcgctg
     3841 gccttgaaag aagagggaga gaagattctc gaagccggat gcaatgcaca tatgacgaag
     3901 cccgttaaac ggcaaacgct tctcgaagta ttgcacgctt gcacaggaca ccgaaaaaca
     3961 tgacgccaca atcagcgaat aacagcaccg gcaaggtgat tgtcacgatc gatcagggtc
     4021 tagaggagat tgtcccagga tttttggaga accgccgcaa agacgtcgcc tcacttgaaa
     4081 ccgccatgca gaaaaacgac ttgaagactg tacgcctgct gggacaccgc atgaaggggg
     4141 atggaggagg ctacggcttt caggagatca gcgccatcgg cgacgtgctc gaacacgcgg
     4201 ccatgcgaga agaccgtacg gtcatcacgg agcagatcaa caaactcgcg gatttcctcg
     4261 cccgcgttga aatcgcatac cgctgaaaac tttcagccgg tcagatcgaa taaccagcaa
     4321 gccaccgaac acacccatcg ttgtgtttcc agttccacgc gcggccatag tctctaacaa
     4381 gcccctatgg aaccgcatcg cccgccacag tgagacccac gctagtctca gcggctattg
     4441 atcacaacaa tgagatcctc tactgcatag ccggacaggg cctcgtcgag cacaacgccc
     4501 ccgttaagga ttggacggca gtgacggatg gtcgacgcgg ctccaaccct tgcgctccat
     4561 gcaggtttgc atcggatcga ctcgcgccat tgtttcctgc gcataggcca gaccgctgac
     4621 atgttggctg agttcttgat tacaggccgg ttcgtcctgc ggcacggtct gattactact
     4681 cttattctcg aactgcatcg gctcgccgca gccagcgcac atgaaaagca atcctgatgc
     4741 aagccacgtt cggggctgaa ataacctgtt ctctgtacct tctctgttga ccatatagtg
     4801 tcgttttgcc tcttgtagga tctcatctgc cgggagaggc gctctctctt tggaaagagc
     4861 gcctctccca tacccagcga accatctcct actcaccaaa gtagccagaa ggatgggcgc
     4921 agcatcgacc ctggcaaacg cgcggaaatt gtggacggaa tccgacagcg cctgcgctcc
     4981 catgacggcg tgctgtcgcg catcaaaaca ctgaaaggac agattgacaa ttttctgcgg
     5041 gagagcggat ggtcgaggca gacacggttg tcaagtcctc cggccgtcga atccgcacgc
     5101 gagccgcagg ttggcgcaga gctctagcgg cacgatcggc aatcgagatc attctctgtc
     5161 cccggccatg gcgcggacgg actggttgac gggacactta attcccgccg gctgaaattg
     5221 tcgtacatga agcctgagcc agctggcccg gtgtaatagc cgatccctgg aaaggtttga
     5281 taggtgcgcg tctcctgccc tttatcgtcc cagtagctcg tcgttccggt accccacggg
     5341 atgaccgtga tcgggccgga atccgctggc aacggcggca ccatcgggcg ctcgcgagat
     5401 tcggcctgga cgatcgcagg agcaagaagg aggaagctga tgagcaagaa ccacatacgg
     5461 cacctcctca gcgagtgggc tgtttggcca tatcgtacca ttaggacgcg agagtgcgat
     5521 aggcgactcg tgcaaggagg cacgggaacg aatattccgc cgaccgtgtt ttctaaatgt
     5581 gataattaaa ctctgacgaa gagagaggct atgtctacat tcgcgctggt cgagagagca
     5641 gggagccgag gccccctgca acccgaagga tcaaagcatg ccaatcactg aatgctcaat
     5701 acacatccgc attcatcgga ccgcgcacac accagtagtg gaggccggga aggaagccct
     5761 tatcgaggtc gcccacggtg aacgtatcga agcccacgat atacgcgttc gacggagcaa
     5821 ctgcttccgt cgtcgccgtc taataagtat ccaagacccc agtgaagaca ctcgtcggaa
     5881 caaaagggat ctgcagcgtg ggatctcgga ggctgctaag ttccgcctcc gacggcagcc
     5941 gccaccccac cgtctttccg acctgcttgt taagacaatt ggatgtcgct atccgccaaa
     6001 tgacgagatc agcacttggc gcctgctccc acacgagccc ggtgttgttg tcccgcaccg
     6061 ctgcgccacc gaagacggtc aagacagtaa atcgcgaggc gctaggcaga ttcttgtccc
     6121 agttctgggt gacgcctgca aatggaccct ggggtgcatc tggagcggcg gcaactggag
     6181 tgatttccag attagcacta aggaggcata cgcctgcgac aagcgcgacc atccgaacgc
     6241 gactgaaaac tcttctgctc atattgctcc tattgtgagg tgaaggtgaa gagcgataac
     6301 agaatggcgg ggcaacgcta tgcctggagc caccgcgcag caataccgca tcggaagtag
     6361 ggagccgtcc gacacaaata acgagtcacg ttacagagcg ggaaaatgca catatgacag
     6421 ccgtgtcatc atcacacgat cggcgcaagt attagcggga aggtccgaac ggaggaaccg
     6481 tcctgggcgt gatgccaggt cgaaaacagt tttcggactc cagctgtctc gattccgcga
     6541 gcacgctacg gtgctagttc cgcaagcagg gctatccagc cggaaacgat tatcaggctg
     6601 aggagcgctg ccacgaagat gtcgacatag tgttccatga cgtgcctccg ttgccaggcg
     6661 gctgccgtca ccctgaatgg tcggtcgaac gcgccgcccg ctactgtaat tgggtcaaaa
     6721 acacgatgag cgccatggac gggatgagga caatgatcgt gtccacaatc aggtccacca
     6781 accagtgtgc catgacatac ctcccgcgaa aggagccaga gcagcagact agagcccgat
     6841 ggagcgaaga ggagaaagcc aataatagat gaggagagca gaggcaataa tatatcccac
     6901 taacaccaca gttctgagca ggacggactg gtcgcgcggg cgatcccgat ctgcccgccg
     6961 ggtgtcgaca gcattgctcc actcgccaac atgtagtgtc attgttcttg ctcctcgctc
     7021 agcatgtcac gatcgctcat gcctactcct tctaattgat acgcggcctt cgaccagaag
     7081 tcgcgcgcgg agtcgttcac ctcgacgatg taggtcgcgg gcgtctcctg atcgtggggc
     7141 ccctagttta ggtgatctgc ccctcggcat tggggcactg gatctgctgc gcagcgcagc
     7201 caagcagcaa gcacacggaa tccagacgat gcacatcttg tttaattggc ccatatgcca
     7261 cctatgccac ctcaggattt agaagatggt aagcgtcttc ttcatattct ccttggtcgg
     7321 cacaaatacc actggcaatt tgttggccaa ggtgcaggcg agatctgaat gaagcaaatt
     7381 ccaccacttc gattcatgat cgttgggcgt agggtcgatg acagaagaaa acctgtcctc
     7441 ttaagagaca gtttgtcccc ctctggtcaa gtccgttgcg ggtgttccgg gcggcgtgac
     7501 gctcgaagtg tagggctgga gaaaatgtgg cgcgaaagga caacggaaga tgtggcaact
     7561 caggaaagag acgttgtgac ctcaggtcgc tcagtattga gcgtgggggc gaaggtggca
     7621 gtacctctga tgcgggtgtt ggcgccggta gtcgttgccc tagtccgaat cccaacagcc
     7681 cgatgccatc accgctcgct gttggtcgaa atgccgggcc ggatatgggt tagaggttcc
     7741 tgcagcaacc catgtccagt ctgttggcat caccgacacg aaccagccgg cagtcacttc
     7801 tccgatgagg atacagctaa tttgatcaga taatttctcc ctatgagact ctcgaaaaca
     7861 aaaagtacct tagcccggcg aaaacaccga ccacaatgaa cccaaggatg agcccaagga
     7921 caggcaagag tgcttttcga acccgggcat ggttcttaaa ggcccaattc catgccggga
     7981 gaatagtctc gatggcattg tccacccctg cacggtatcc aatgaaatac caccgatcca
     8041 gatttctctg aataaaaaga aacaccctgt tggaaatata tgcagctgcg cccgggctgt
     8101 ttctcggctc aatttcgggg cagcctttgt cgagaaagcg ctggaaattg tctatgggaa
     8161 taaaaagttc atcgtgctca taccaactgg ttaagctcca gcctggaaga cacttcacaa
     8221 agtattctgg cactgctcca agtgtattcc gcagaccagt tccatagcca atgaccaaag
     8281 ggagacaaac aacagccaca atgaaccctg ctaccgcaaa gagcaaaaaa cgagttactt
     8341 taagggtatt actgtcctgc attcctattg cttccgcttc tcctggtact tcttcaggag
     8401 atcgtgaaag gatctatttg ccgttcggca agaggttcgt ccgcagatta ttgtcccagt
     8461 cttgggtgac acctgcaaat ggactttcgc ttgacgattg tgatgtcaca gatcctgatc
     8521 ttcacaccca tagtctgtcc aaactacgaa cactgactgc gacttcgcct ctccccgaaa
     8581 aggtctcgat aggtccactg acatgcgttc cctttggcgt caaactggac ggtgagtgtt
     8641 cccacttcat tcggcaatcg aacaggccat tcacaggcct
//
LOCUS       DATIFR010000232         4327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_353364, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000232 DATIFR010000000
VERSION     DATIFR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353364"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VLD60_13140"
     CDS             <1..208
                     /locus_tag="VLD60_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45953.1"
                     /translation="KPEVKRFVEFYLAQAPALVPQVKYVPLPQQAYELALSHFRSGKI
                     GTAFQGVSTIGIKIEDLLRREATL"
     gene            221..1165
                     /gene="pstC"
                     /locus_tag="VLD60_13145"
     CDS             221..1165
                     /gene="pstC"
                     /locus_tag="VLD60_13145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HSB45954.1"
                     /translation="MDVDVLEAGIVKKVAESSGIPRMWHQWKEKGIELLLLIAALTSV
                     AITIGIVGVLAYESVLFFRQVSLVEFLTDRQWTPLFAEPHYGILSLVSGTLVTTIVSL
                     MVAVPMGSLIAIYLSEYASRTIRESVKPALELLSAVPTVVYGYFALLFVTPMLQKVWP
                     ELPGFNMLSAGLVIGIMIVPYVSSVSEDAMRAVPVHLREGAFALGATRMQTALRVVFP
                     SALSGITAAYVLGVSRAIGETMVVAIAAGMQPTLTWNPLEPAATMTAYIVQVSLGDLP
                     HGSIGYQTIFATGLTLMLITLVFNIAGHVLKKRYRQVY"
     gene            1242..2075
                     /gene="pstA"
                     /locus_tag="VLD60_13150"
     CDS             1242..2075
                     /gene="pstA"
                     /locus_tag="VLD60_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSB45955.1"
                     /translation="MSIALLVLLALLGQLIFDGLGRLSWQFLSSFPSRFPDQAGILSA
                     WVGTILVMFLTALTAIPLGIGAAIYLEEYAPKNWVTELIEINIANLAGVPSIVYGLMA
                     LGLLVYELHLGQSFLTAGLTLAMLILPMVIIATREAIRSVPPQIREAAYALGATKWQT
                     VRDHVVPYSMGGMLTGMILALSRAVGETAPLITVGALSFIAFLPHPPWQAEFPFISLQ
                     WLFDPFTVMPIQMFNWVSRPQEEFHVNAAAAGLVLLLMTLAMNALAIIIRARFRKRIH
                     W"
     gene            2089..2865
                     /gene="pstB"
                     /locus_tag="VLD60_13155"
     CDS             2089..2865
                     /gene="pstB"
                     /locus_tag="VLD60_13155"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSB45956.1"
                     /translation="MAASQDVLKAESRALSFYYGAAQALHNVSLDLYDRRVTALIGPS
                     GCGKTTYLRCLNRMHDLYPGNRYEGEVLLYPDKVNIVGPGVDPIEIRMRIGMVFQKPN
                     PFPKTIFENVAYGMRIRGTSARHDLDIVVERALRNAALWAEVKDRLHGPAFNLSGGQQ
                     QRLCIARALATDPELLLFDEPTSALDPTATARIEELVTELKEKVAIIMVTHNMQQAAR
                     VSDYTAFMYEGRLVEYARTEKMFTNPSVKLTEDYVTGRFG"
     gene            2886..3566
                     /gene="phoU"
                     /locus_tag="VLD60_13160"
     CDS             2886..3566
                     /gene="phoU"
                     /locus_tag="VLD60_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSB45957.1"
                     /translation="MQRHIDQELADLKTQLLKMGALVEEQIERAIKALVDRDSELASQ
                     VIERDKLVNRFDVEIDENCIRLLALQAPAARDLRFVTTAMKISTELERLSDLAENICE
                     RVIELNEEPQLKPYIDIPRMANWAMKMVRESLDAFVRSDGTLARKVCADDDFVDDLTH
                     QLFRELLSFMLENPATITRAIRLTFIGKYIERIADHATNIAELVIYMVEGKIVRHMEP
                     PAVQGKIS"
     gene            complement(3570..3806)
                     /locus_tag="VLD60_13165"
     CDS             complement(3570..3806)
                     /locus_tag="VLD60_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45958.1"
                     /translation="MRIVRIIGVVCALGLVSACSNTRWVHPTKPESEYTNDYNRCQNL
                     ILRDPKLQQGNQLLVVNATERCMQKEGWRLVEDE"
     gene            4000..4075
                     /locus_tag="VLD60_13170"
     tRNA            4000..4075
                     /locus_tag="VLD60_13170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4033..4035,aa:Thr,seq:tgt)
BASE COUNT          889 a         1215 c         1230 g          993 t
ORIGIN      
        1 aaagccggaa gtcaaacgct tcgtcgagtt ttacctggcc caagctccgg cccttgtgcc
       61 gcaagtgaaa tatgtcccgc ttccccagca ggcgtacgag ctggcccttt cacactttcg
      121 cagcggcaag atcggcaccg cctttcaggg cgtgtcgacc atcggcataa agatcgagga
      181 cttgttgcgc cgcgaggcta cgctctaaaa gatgacgcca atggatgtcg atgtgctcga
      241 agctggcatc gtgaagaagg ttgccgaatc ttcggggatt cctcgcatgt ggcatcaatg
      301 gaaagaaaag ggaatcgaac ttctgctctt gatcgctgca ctcacgtcgg tggcgatcac
      361 gatcggcatt gtcggggtgc tggcctatga gtcggtcctg ttctttcggc aggtctctct
      421 cgtcgaattt ctgacggatc ggcaatggac cccgctcttc gccgaaccgc attatgggat
      481 tctgtccctg gtgtcaggga ccctggtcac gacgattgtg tcactgatgg tggcggttcc
      541 gatgggaagc ctcatcgcga tctacctgag tgagtacgcc tcacgaacaa tcagagaaag
      601 cgtgaagccg gcccttgaac tgttgagcgc cgtacccacc gttgtctacg gctattttgc
      661 gctgctgttt gtgacgccga tgctgcaaaa ggtatggccc gagctgccgg gcttcaacat
      721 gttgagtgcc ggcctggtaa tcggcatcat gatcgtgccc tatgtcagtt ccgtcagcga
      781 ggacgccatg cgagcggttc ccgtccatct tcgagagggc gcctttgcat tgggagccac
      841 gcgcatgcaa accgcgcttc gtgtcgtgtt tccctctgcc ttgtccggga tcacagccgc
      901 ctatgtcctg ggagtctcac gggccattgg agaaacgatg gtcgtggcga tcgctgcggg
      961 gatgcagccg accttgacgt ggaacccgct tgaaccggcg gctacaatga ctgcttacat
     1021 cgtacaggtg agtctgggcg atttacctca cggcagtatc ggttaccaga cgatcttcgc
     1081 caccgggcta acattaatgc tcatcacact ggtgttcaat attgctggcc atgttctcaa
     1141 gaagcgatac cgacaagttt attagtccca gcgcaattca gcgtctgatc gcccgacaca
     1201 aacggctcga tcgtttcttt gcgctcaagg gaggcgcagt tatgtcgatt gccctgctgg
     1261 tgttgcttgc cttgctcggg cagttgatct ttgacgggct gggtcgtctc tcctggcagt
     1321 tcctgagttc ctttccatcg cgctttccgg atcaggccgg gattctgagc gcctgggttg
     1381 ggactattct ggttatgttt ctgacggcgc tgacagccat cccgctgggt atcggtgcag
     1441 ctatctatct ggaagagtat gcgccgaaaa actgggtgac cgagctgatc gaaatcaaca
     1501 ttgccaattt ggccggcgtt ccgtccatcg tctacgggct gatggcgctc gggctcttag
     1561 tgtacgaact gcatttaggg caaagctttc tcactgccgg tcttactttg gccatgctaa
     1621 tcctgccgat ggtgattatt gcgacccgcg aggccattcg gagcgtaccc ccgcaaattc
     1681 gtgaggccgc ctacgcgctc ggggccacta aatggcagac cgttcgggat cacgtcgtac
     1741 cctattcgat gggcggcatg ttgaccggaa tgatcctcgc gctctcgcgc gcggtcggcg
     1801 aaacagcgcc gctcattacc gttggcgcct tatcctttat tgcctttctc ccccacccgc
     1861 cgtggcaggc agagttccca ttcatttcct tacaatggct gtttgaccct tttactgtga
     1921 tgcccattca gatgttcaac tgggtttcca ggccgcaaga ggagttccac gtcaatgcgg
     1981 cggcagccgg actggtgtta ctcctcatga cattggccat gaatgcgctg gccatcatca
     2041 tccgcgcccg cttcaggaag cgaatccatt ggtgacgcga tgaatgcgat ggctgcctca
     2101 caagacgtgc tgaaagcgga atcccgcgcc ctgagcttct actacggggc ggctcaggca
     2161 ctgcacaatg tctcgcttga tttgtatgat cgccgggtga cggcgctgat cggtccgtcg
     2221 gggtgcggga agaccacgta tcttcgctgc ctcaaccgga tgcatgacct gtatcccggc
     2281 aatcgctatg aaggggaagt acttctctat ccggataagg tcaacatcgt cggaccgggc
     2341 gttgacccga ttgagatccg tatgcgcatt ggcatggtct tccagaaacc caacccgttt
     2401 ccgaaaacaa tttttgaaaa tgtcgcctat ggcatgcgca ttcgcggcac atcggcccgc
     2461 cacgacctgg atatcgttgt ggagcgggcg ctgcgcaatg ccgccctgtg ggcagaggtc
     2521 aaagaccgcc tccacgggcc cgcctttaac ctttcgggcg gacagcagca gcgtttgtgc
     2581 atagctcggg ccctggcaac cgatccggaa ctcctgctgt ttgacgaacc gacatcggca
     2641 ctggatccta ctgcgacggc ccgcatagaa gagctggtca ccgagttgaa ggaaaaggtg
     2701 gcgatcatta tggtcacgca caacatgcag caggccgccc gggtgtcgga ttacacggca
     2761 ttcatgtacg aaggacgatt agtggagtac gctcgaacag agaagatgtt taccaatcca
     2821 tccgtcaagt tgaccgaaga ctatgtcacc ggtcgattcg gctgaccgcg aaacaggacc
     2881 tcgccatgca acgacacatc gatcaagaac tcgccgacct caagacccag ctcctcaaga
     2941 tgggggcgct ggtggaagag cagatagaac gcgccatcaa agccctggtg gatcgagatt
     3001 ccgagttggc gtcacaggtg atcgagcggg acaagctggt caatcggttc gatgtggaaa
     3061 ttgacgaaaa ctgcatccgg ttgctggccc ttcaggcgcc ggcggcgcgg gacctccggt
     3121 tcgtcacgac ggcgatgaag atttcaaccg agctcgagcg cttgagcgac ctggctgaaa
     3181 atatctgcga gcgggtgatt gaactgaatg aagagccgca gctgaagccg tacatcgaca
     3241 tccctcggat ggcgaactgg gcgatgaaaa tggtgcgcga atcgctggat gcttttgtac
     3301 ggtcggacgg gacgctggct cgaaaagtct gcgccgacga tgacttcgtc gacgacttaa
     3361 cccatcagct gtttcgtgaa ttactgtcgt tcatgctcga aaatccggcc acgatcaccc
     3421 gtgccatccg cctgacgttt atcgggaagt acatcgagcg gattgccgat catgccacca
     3481 acatcgcaga gctggtgatc tatatggttg aagggaaaat tgtccgccac atggaacctc
     3541 ctgccgtaca aggcaaaatc tcctagaggc tactcgtctt ccaccaaccg ccagccctcc
     3601 ttctgcatgc agcgctcagt ggcgttgacg accagcaact ggttaccctg ttgaagtttg
     3661 gggtcgcgca gaatgagatt ctggcagcgg ttgtagtcgt tcgtgtactc ggattccggc
     3721 tttgtgggat ggacccacct cgtgttagag cacgcgctca cgagtcccag tgcgcagaca
     3781 actccgatga tccggacaat tctcatccct acctccaggt tataggagca cgatagcgag
     3841 aagatagttg gtggacgaag ggaacgtcaa gggctgaggc gagggatagg caggcatagt
     3901 cctcctggag attgagacca gatttcttga cagaatcttc agcgaattgc tagggtgtcg
     3961 aaatcaaatt ggtagccgaa cagttccaac ctttcacggg ctggcgtagc tcaatcggca
     4021 gagcagcggt tttgtaaacc gcaggttgga ggttcgattc ctctcgccag ctccacaact
     4081 tccgctgacg tttcaacctc ttggcggctt acagaactgt tgcgctcccc tctcaattca
     4141 gccagtgtac ccgcgagtgt aaccgtctgc ttttcgaaat aagcagagag cgactgcgcg
     4201 gcttgttgca ggtcgttctc attcacgatg ttgtagcggt caaagacgct ccgggtcttg
     4261 tgccctgaga tcgccatcgc taccttttca ggtacacccg cccggaccat gttccgaacg
     4321 gccgtcc
//
LOCUS       DATIFR010000233        30679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1310850, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000233 DATIFR010000000
VERSION     DATIFR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 30679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30679
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310850"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VLD60_13175"
     CDS             <1..192
                     /locus_tag="VLD60_13175"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSB45959.1"
                     /translation="ELKKQWGKFTDDDLMQIEGDYDKFIGSLQKRYGDQKEEVRRWVE
                     QWYQRREGSGKPGTMPKAG"
     gene            275..556
                     /locus_tag="VLD60_13180"
     CDS             275..556
                     /locus_tag="VLD60_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSB45960.1"
                     /translation="MKKVFIASVLMAGLMGSSAWALFETNQELAKSATVTLDKAVLAA
                     VAAMPGKAVEAELSKEEGRTVYKVEIIDHNNKTQKVYVDAQTMQTRIDK"
     gene            778..993
                     /locus_tag="VLD60_13185"
     CDS             778..993
                     /locus_tag="VLD60_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSB45961.1"
                     /translation="MNKDQFQGKWKQFKGDLKKQWGEFTDDDLKQIEGNYDKFEGKVQ
                     ERYGDRKEDVKRWTDEWFKKQSGSKRN"
     gene            1030..1257
                     /locus_tag="VLD60_13190"
     CDS             1030..1257
                     /locus_tag="VLD60_13190"
                     /inference="COORDINATES: protein motif:HMM:NF010229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HSB45962.1"
                     /translation="MFSARSTKTLDYRGKEGSGPMLYYALLFLIVGIVAAVLGASGVA
                     AVATQISWVLFVVGMVLLLIHLLSGRTPPVT"
     gene            1320..1748
                     /locus_tag="VLD60_13195"
     CDS             1320..1748
                     /locus_tag="VLD60_13195"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HSB45963.1"
                     /translation="MRQNCIIREVFSWLVIAGLGLSLTACNTTKATMDTTVNFFSSTS
                     PGSMFTADGMVEQKQKVNLFAGVAFENLRQDMAQGGGPYLTSLAVLMNIPSDRQDEFA
                     RFVQDRYPVLFTSDLATDHTAHLKMLAVLDRELETYERAR"
     gene            2043..2459
                     /locus_tag="VLD60_13200"
     CDS             2043..2459
                     /locus_tag="VLD60_13200"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HSB45964.1"
                     /translation="MRTIMDIRIPIRITAWLFVVLTFALCMSPLESRASLVPSVPSDG
                     SVVSIPESGMDRVEDLRTIQHVLETKVVQQRLRDLGLSREEIDSRLDRLSDAQLHQVA
                     TQINSLLPGGGIDGALSTILTVLLIILVAVVILILI"
     gene            2489..3019
                     /locus_tag="VLD60_13205"
     CDS             2489..3019
                     /locus_tag="VLD60_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HSB45965.1"
                     /translation="MAFAAWGATVTGCAGSPGVSTDALHPKVHHLVGVPFFPDSTDQC
                     GPATLASVLTFWGAPTDPRSLREEIYLNHLRGTLPLDLLLAAEARGFKAEAYLGSTDN
                     LREELAAGHPVIAFLDLGFLMFPQGHYVVVTGYDAQRQGFYVHSGLERDAFLSDSRFV
                     KSWDKTSRWTLRVLPQ"
     gene            3038..3658
                     /locus_tag="VLD60_13210"
     CDS             3038..3658
                     /locus_tag="VLD60_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB45966.1"
                     /translation="MSRLGRHMYMRSGICAMILAGAASACSAQRAIMPADPLTAEEHV
                     RLGFIYETQHLNDLADQEFQATLRQRPRSTTALVGLGNLAFERGAFGEAQDYYNRALA
                     IEPDHPGANNNLAMIYLTLGSRVAEAERLALTALNRAGPLRPYVLDTLAGIYRRQGRE
                     RDAQDALKEAEALMPGDSTVLRNQIDSREPNSTVRSQEGADAVQPR"
     gene            3892..4449
                     /locus_tag="VLD60_13215"
     CDS             3892..4449
                     /locus_tag="VLD60_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSB45967.1"
                     /translation="MKKKFNKTFPNDVMAVRRRAREHTDGGAVTEGYRADRKMVLKLL
                     NEALATELVCVLRYKRHYFMADGIYAGPVAQEFLAHAADEQAHADQIAARIIQLGGEP
                     DFNPEGLLTRSHSEYVEAESLVEMIKENLVAERVAIDSYAGMIRYLGDDDPTTRRMAE
                     DILAKEEEHAEDLASFLATLDVSKR"
     gene            4701..4988
                     /locus_tag="VLD60_13220"
     CDS             4701..4988
                     /locus_tag="VLD60_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45968.1"
                     /translation="MSSNCRPFTIMLPISFLVGGLAGLDAIPPTVEGPAGRITNAVAM
                     THTNTTVLVLVASRSGGKTEIDQTKADAYTRLELDFRRDYESKYADSGYAY"
     gene            5542..5919
                     /locus_tag="VLD60_13225"
     CDS             5542..5919
                     /locus_tag="VLD60_13225"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HSB45969.1"
                     /translation="MTKKSDRLIRIQDAEELVRDFIEAEISRADADDFYFLNQSEEWS
                     HDLRASQRALPDAGDAALLGGNAEAAWDEDEIGNEGVGGSILEPDQDLIEQMNEVFEL
                     TYEDNGLFWLVERSEWRDAHHWN"
     gene            6781..7188
                     /locus_tag="VLD60_13230"
     CDS             6781..7188
                     /locus_tag="VLD60_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45970.1"
                     /translation="MPTYKTPSVEKSTHNRLPHRRHMTMKIILCLTLAPWLLPPFAGE
                     ANTYDAMTQSAKPYEVTGTLLELDLSKGKGLLRTDLGKRIYLEVRKPELFQNLSVGDR
                     LTIQLNGDGQVDKVLGVEVPELSITGRPVPPQP"
     gene            7202..7840
                     /locus_tag="VLD60_13235"
     CDS             7202..7840
                     /locus_tag="VLD60_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HSB45971.1"
                     /translation="MKQAGTYISRPFNLHGLSGISDRTIDMHMKLYEGYVKETNHLTQ
                     LLQDILKDGRVDQEEMPAYSELKRRLGFEYNGMVLHEYYFENLMRSASNGPSRNAPFR
                     VAAEESFGSYETWKADFIGVGKMRGVGWAICYQHPQTGSLSNHWITLHEIGNVTGYMP
                     ALVMDVWEHAFLLDYQPSERGEYIEAFFGNINWRAVDERVTRSQAAVTAHSA"
     gene            8416..8745
                     /locus_tag="VLD60_13240"
     CDS             8416..8745
                     /locus_tag="VLD60_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HSB45972.1"
                     /translation="MHTIILLTVSNIFMTMAWYGHLKYKDSPLWIVILVSWGIAFVEY
                     CFQVPANRIGHYEFTAAQLKTIQEVITLVVFCIFSVLYLKEPLKWNYLAGFGLMVGAV
                     FLIFKEW"
     gene            8901..11435
                     /locus_tag="VLD60_13245"
     CDS             8901..11435
                     /locus_tag="VLD60_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB45973.1"
                     /translation="MAPKKKETTKMKDSAKSAAAPASSGSAEDFEKLGVFYLGRPYDL
                     AAKRAKPGWLLYDSKDLVTHAVCVGMTGSGKTGLCIGLLEEAAIDGIPALIIDPKGDL
                     ADLMLTFPQLRGEDFAPWINEDDARKKGLSPADYAKGQAELWKKGLGDWGQSGERIQK
                     LRDAADVVVYTPGSNAGIPVSILKSFAAPSQEIVDDAEMLRERLSTTVTSLLGLIGVE
                     ADPIKSREHILLSTILDRTWKAGQNLDLASLIQQIQTPPIKKIGVLDLDSFYPSKDRF
                     ALAMQLNNLLSAPGFQSWLEGEALDVGQMLYSRGGKPRLAILSIAHLNDAERMFFVTL
                     LLSQTVGWVRAQSGTTSLRAILYMDEIFGYFPPVANPPSKQPLLTLLKQARAFGLGVV
                     LATQNPVDLDYKGLANTGTWFIGRLQTERDKARVLEGLEGAAASSGKKFDKQGMEQIL
                     AGLGNRVFLLNNVHEDAPEVFQTRWTLSYLRGPLTRTQIKTLMDPVKRETLGVRGKEE
                     STKLGSLEISSHVSRLTPYASRPMLPPDVPQQFMPVRGSQPSGSALIYQPMLLGASQV
                     RVSDSKAGIDVTQDVTVLVPISDGAVAVDWDHAAEAAVALGDLESSPVEGAQFAPVPS
                     AASKAKSYEVWSKDFSSWLFRTQKVDLLKSPSTKVISKPGESERDFRVRLQQGGREQR
                     DKQSEGLRQKYAPKIASLQDRIRRAEQMVERQQVESRSSQVQAAISVGATILGAFLGR
                     KAISATNIGKATTAIRSAGRVMKETQDVGQAQENVAVLQQQLADLEAQFKAEADALAA
                     TTDPLNEKLEAISLKPAKANIAVKLVALAWTPHWQDAKGTMSSAWL"
     gene            complement(11466..14321)
                     /locus_tag="VLD60_13250"
     CDS             complement(11466..14321)
                     /locus_tag="VLD60_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB45974.1"
                     /translation="MPSPQGPHAPLRLPTILEEEYLKLHTSPAADSTATLKSEYKKLP
                     HPSFEDYPVNGSEDERLKAVYELIHGLPDRRSALCFSGGGIRSATFGLGVLQGLARTN
                     LLERFTYLSTVSGGGYIGSWLSAWIKRHPQRIAGVAEELRRQPQTPLDTEPLPVTWLR
                     RYSNFLSPRLGLLSVDSWTLIGTYLRNLLLNWLVLLPLLVVGLMVPRFMVALVRWNLS
                     DMGLPESLMKVLFYAGLGLIVVAMSYLHLYRPSLEPYRPENLKKPIERQKWKPFEPQK
                     WFLTLSLIPTLLSVLLLTTAWAWFRNAGGTLHQLAVDGLGSRETFMVGALLIHLISWL
                     LSALLLRRFKHLSRWLFVEILVIAGTGALGGFFLWSVLAETPSEMPVSSFAEWYAGFA
                     VPGVLAVFLLTATLFVGVASRYTDDDDREWWARSGSWVLLAGVVWVAAATLVMFGPGI
                     LAFTPAVITSLGGFSGLITLVLGFSSRTAPTTSQRNRRSVKVAEYAVKLATPVFIVFL
                     LTVLALGTSWLLKILAHLHGVLAVDLQSIGLQMLSDPWGHANVIHNAPFWLLVETSLA
                     LTALGCSMAWVININKFSLHSMYRNRLILAYLGASQLEKYGVKRTPNLFTGFDPDDNI
                     QMHDLAPPGQPISKPFHVINIALNLVRGTNLAWQQRKAQSFTVSPLHCGSFASHLGYR
                     SASRYGTNSGVGKAITVGTAMAISGAAASPNMGYHSSPAVAFLLAFFNVRLGWWLGNP
                     GDAGARTYDRSCPEFSVGPLLAEAFGLTDENSRYVYLSDGGHFDNLGLYEMVLRRCHC
                     ILVSDAGADPDFAFQDLGNAIRKIRIDLGVDIDIKLERLKRQESTRHTSGHHGIGTIH
                     YSRVDPGAEDGILIYLKPSLTGEEPVDVLEYASNHECFPHEPTTDQFFDESQFESYRR
                     LGEHIVEEAFTTALATSQPALSKVFETLRDRSENPRA"
     gene            complement(14332..15771)
                     /locus_tag="VLD60_13255"
     CDS             complement(14332..15771)
                     /locus_tag="VLD60_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB45975.1"
                     /translation="MNVLFALGLGFSLTLTGCFAKIQQLPAHPDRNTPVFAPLADQET
                     LVGLAVSGGGSRAATFAAGALEALAEIRITRDGKERSALETVTHMSSVSGGSLATAYY
                     TMTKPPKSVPVLGDQGLSPVYRNFFSDFKTAMQMDFQSRAAVRQLLFFRAFNPTKFAY
                     SFSEVWDSNFFHEMTFSQLYAREQRGDSPRVILNGTVYNSGQRFALTTLPSEEFEYDF
                     IELLIKELKKPDRPVPVTPEGLAIIQSGLEKAKKQFLPLTFDQIGANHENLRVSLAVA
                     TSASFPPVVGPVTYNVDGRPPYHHIGDGGLFDNLGTESLTTLFLKKIPQGSSKKGLII
                     VIDTSFPFDANAGELDNSEKGFEVFKDDPSRIVGIMEERANVYQTLLWHSLRTEGVVL
                     PDFAHLRIVVLKHTDADWTGYQDLPEACRGEFSQNVTPKQIKQAVGQIPTLFKIKTPC
                     HGALLVKAAQKVVDKYRPRIVKWLETPQS"
     gene            complement(15817..16941)
                     /locus_tag="VLD60_13260"
     CDS             complement(15817..16941)
                     /locus_tag="VLD60_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45976.1"
                     /translation="MQCWNVLPRIAVATLIVLAGCASPILQLGEQSGLSEKVKGNAEL
                     AKQFLSCSRQAYGDQTAESASATPSITPGGQAPSVAPALPAVEAAVADSPQLKTDEDI
                     HGLLDLIRDMDDPTERKVDIGRLIRIAEMGKRLHARFRIDEDRLAQDKSLFAQLLLAY
                     NKAYFGDIQFRTQPTQTGSGIQGVFKVVSAGFVDRNGNAFAFLGLSSEVTVSMDPVKQ
                     EPTVRFHASSVDSRHVASDLVRIFLEALFDAAFRVPATQEATALKVKWSRDERPYPRI
                     DPDHPPISYEQFARVARDAMRAEAAVTSAVGKAVRGGGVFETNNETLAAAVETAGGVL
                     AKKLAEHEAFCYFQVTKGKPLVMNHADTKTDALAVLTATR"
     gene            complement(17143..17691)
                     /locus_tag="VLD60_13265"
     CDS             complement(17143..17691)
                     /locus_tag="VLD60_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HSB45977.1"
                     /translation="MSDQLPASNSQAADHPIENVLETVVFASRWIQAPLYAGLIVAEL
                     LYAYKFLIELWEMAIHIQQLEETKFMLGVLGLIDVTMVANLLTMVVIGGYATFVSKLD
                     LETHPDRPDWLTHVDPGTIKIKLAASLIGISSIHLLKSFVDIANEDYEHVKWKIFIHM
                     TFLGSAILLAWTDKIMQKEKKH"
     gene            complement(17803..18210)
                     /locus_tag="VLD60_13270"
     CDS             complement(17803..18210)
                     /locus_tag="VLD60_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB45978.1"
                     /translation="MNILIADQDPLFRQRLKRRLEKISGVTVIGETAEAEATTVMIVN
                     RKPDVIILNIDLKNGSGIEVLRHITRLILHPTVIMVTNQLSQELRSACAVAGADFLFD
                     KALEDRKVVNTVRLLCLPEPEQDLSDPAEALPD"
     gene            complement(18443..21487)
                     /locus_tag="VLD60_13275"
     CDS             complement(18443..21487)
                     /locus_tag="VLD60_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB45979.1"
                     /translation="MSAPLEVSPALVEELQQLRQRVAELEGQRTSDPPPTGITTQPSS
                     DHYRLLYEDTPSMSFALSPDGTVLSVNRFGADQLGYSADTLTGQSVLVVFDPADHQTV
                     REQLRACTSHPYKIFQWNIKKVRRDGTRLWVRETARAVRDENGSLLVLVICEDITAQK
                     AAEEENRITVQALQALVHMSPLAVMALDTTGETVTLWNQAAESMFGWRADEVIGHPAP
                     FLPSDKREESDRLWARLLQEGSLSAVELRRTRRDGSPIDLALWATILKDPQGIVTGTL
                     GFLADMTERKRAIDALRESEQRARALFEQAPLGIGILDSVTGHFQQINNKYCEITGYS
                     EEEMLARTFQMITHPDDVEADVNNMARLLDGEIHTFQMEKRYIRKDGSTVWVNLTCVP
                     LWLDATDRRLHIAMVENITERKGAEQELRMIRERLQHLMASSPTVIYSAKTSGDYGAT
                     YVSENILEQLGYEPREFVDDSGFWATHIHPDDKPRVLANLSCLFRDGHHVDEYRFLHK
                     DGTYRWMHDRLKLLNDQAGNPLEIIGSWIDITERKRAEEALQKSETRFRQIAETIEEV
                     IWSADPAIGRMLYISPAYERVWGRTCSSLYEHPKSFLESIHPEDRAHVHAGLAVQQDG
                     LPFAHEYRVVRPDGMVRWVWDRGFPVRDRETGCVTHYVGVALDITERKQAEEALRVSE
                     ERFAKAFRSSPHPIIVTELETGCCLEVNEASLRLFGFRREEVAGQSTITLGLWPTDAD
                     RKRFIDRLKADGSLRNLDLTFYTKDRTARRCLVSCELIELNGNQCVVTVGTDITEQKR
                     AEEALRRSEQEVRLAFEERERLSQDLHDNLLQSLYAVGMGLELTKQKTQRLSQTNAKR
                     IEETIDQLNGVIREVRNFIPRVQPPRIQEGTVADAIRSLVHSFMATGAGPVSLSIDDH
                     AAGRLSPERSLHVFAITKEALSNSVRHAKATRRSVTLRQERGKIRLEVTDNGHGFETA
                     RRHNQGMGLKNMRARARRLRARLTIKSALNKGTTITLSLPSS"
     gene            complement(21615..23450)
                     /gene="uvrC"
                     /locus_tag="VLD60_13280"
     CDS             complement(21615..23450)
                     /gene="uvrC"
                     /locus_tag="VLD60_13280"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSB45980.1"
                     /translation="MATSDTLQSKLAHLPEQPGVYLFKDSTGELLYVGKAARLADRVR
                     SYFLKRADHSPKTSLMVSQIADLETMVTRSELEALILESNLVKRHRPRFNVVLRDDKQ
                     YPYLRLPIKDQFPRLSIVRRVQKDGALYYGPYTPAGALRDTLKVIKKVFPLATCTIDI
                     DGKAERACIEFEIKRCMAPCTGNQSKEDYHRIVQQVRHFLEGRDRELLDELRAQMEIA
                     AEGENFEEAARLRDRMFNIERTLERQRITQTASVDQDVVGIARQGAAVDLQLLFVRGG
                     LLIGRKDFFWPQSADATDEELVRSAIEQFYNKEGQPPRELLIPVDIPDAELIGCWLSD
                     KRGETVRVLAPERGVKHQLVLLAEENAAAAVADHLRNEALDRQAGEELKRLLRLERIP
                     HRIEGFDISNTMGNQSVASMVVWEDGRMKKADYRRFKIETVVGANDFASMQEVVSRRY
                     GTVEDLARPDLVLIDGGPGQLAAAMEGLKQVGAQGLPILGLAKARGEKDERIFLAGRK
                     NPLLLKPTSPGTHLLQRVRDEAHRFAVTFHRKLRSRALMTSRLDQVIGIGEIRRTTLL
                     NRFKTLEGILAASDSELKEAGLSAQTIRELRTVAGTAPSSQILPD"
     gene            complement(23595..24464)
                     /gene="dapF"
                     /locus_tag="VLD60_13285"
     CDS             complement(23595..24464)
                     /gene="dapF"
                     /locus_tag="VLD60_13285"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSB45981.1"
                     /translation="MKNGFFRGHGLGNDYIVMDPKELSFKLTPKTVQLICNRNWGLGS
                     DGILALAPSKKADFGLRIFNPDGSEAEKSGNGLRIFARYLHATGKTKKRHFTVETKGG
                     LVTIDLHVDRHGEASAVTVEMGKATFKPAALPCSLPAEELTQQPIEAAGRSLTFTGVS
                     VGNPHCVIFKPAGASWSREELLALGPALENHALFPKRTNVQLAVPTGPKEIYILIWER
                     GAGETQASGSSSCAAASAAVRLGLVKSPVTVKMPGGTLNIEVSQDFSLTMKGPVAEVA
                     RGALSPSFVRGLR"
     gene            complement(24545..24952)
                     /locus_tag="VLD60_13290"
     CDS             complement(24545..24952)
                     /locus_tag="VLD60_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB45982.1"
                     /translation="MGTLKVKKLLHTRMRVSDMDQTIAFYTQVLGLEVVERHTSPRGS
                     HLAFLSVPNSEELIELCSFPPSGPVKVQEDLVHLAFQVDNLDETISALNSKGVRITDG
                     PTKSSSGSRFIFIDAPDGYEVELIERPAGVKIV"
     gene            complement(24996..25475)
                     /locus_tag="VLD60_13295"
     CDS             complement(24996..25475)
                     /locus_tag="VLD60_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB45983.1"
                     /translation="MNRHDIRAMQPGDCEAVVQLLAESDPWKSLGYTVEDWGRIFCPI
                     PQGRDSFVSVVDGTIAAVAIVRQKFLLGDYLELLGVTDQARGKGIGKELLAHIESQVF
                     ARTKNLFACVSDFNSGARAFYKKQGYQEIGPMPNFLIPGSAEILLRKTLGPARERKA"
     gene            complement(25472..26185)
                     /locus_tag="VLD60_13300"
     CDS             complement(25472..26185)
                     /locus_tag="VLD60_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSB45984.1"
                     /translation="MARRRSQLILILLLTVLPLQMTPEPGNAQVIKSGPPACPGIALT
                     FDLCPVRKSPGYDQALVDYLTEHKIPATFFMSGQWIAKHDPEVEQLLGIGFFEVGTHG
                     EVHAHLPMHSAEEQRKEILGPITMLSEHYAHEARLFRPPFGEYDGVTVDLVKSLGLQF
                     IQWNIESGDPDPTLSADQILARIEKRARPGSIVVLHANGKGKQTRQVIQRLTTEVLPR
                     KRLKPMTVSELLSCKQPNQ"
     gene            26288..27439
                     /locus_tag="VLD60_13305"
     CDS             26288..27439
                     /locus_tag="VLD60_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSB45985.1"
                     /translation="MNVPAWEIGRALGIPIRVHPSWFLVFVFMTWTLATGYLPEALPG
                     LSGSRYWGMGAVAALLLFLSVLLHELGHSYVALRYRIPIGQITLFLFGGVAHMKKEPP
                     SPRAEFLIAIAGPIVSFALGAFCLGTIEAVDALPMFSGGRGFVVLGGLLGVNNIMLGL
                     FNLIPGFPLDGGRALRAGLWAWSKNFHQATSQAAFIGLGFGFLLGGFGALLVSGAISG
                     MFDPSLASRGGWLLFIGAFLFGAAWSTRRQVSLRAALSSTSVRDVMIRAVVTVPQHLT
                     LQAAVDEFFVAYGYGGFPVSDEGKVVGLIAVENVQAVPQGLWAWRTVRDVMRPASPGL
                     FIPPDWSIMLAMDRMVQEGWDRLVVVEEGQPVGLITRSAIAQFLQLHKP"
     gene            complement(27470..28816)
                     /locus_tag="VLD60_13310"
     CDS             complement(27470..28816)
                     /locus_tag="VLD60_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45986.1"
                     /translation="MGKELLMLGGAIVGFLGAFLTVLEKILDLRSKLKPEKERKTPAP
                     VERSAHAASLPIDFFSSKPFRGSSYLLLYETSVIVAAGLILNYVGLTLSRHMESILFL
                     DMTGTALVAFLLGPWWGAIAALLSNSVVNWLLFPESGADVVIFPWSLVSMTGAIYWGW
                     MARQAGFQKYLRTGKNSAFSHAWFLYIFGVGGAVVMSIPGTFVQAALNEHATFALNPE
                     FAETLSARVLEWENMVHQWMESIFGLTWGEHVGWWIVNWFQNWVRYVPDKTMSAAIAL
                     VVLKYGYPLFERELIHGGPEGERPKDERILPLVLGLLYAPSFATLVSSETYGGATYWP
                     LWAMPWCLILLGYLYLRYRGPNDSTLQEARLGRAERYARALKPVGKEASYEFCRRLTF
                     MTLIASLIFALCLPVLLMDFYRVTFKFFCVVYGFLLVVHLVRIAIAQNISVARSDG"
     gene            complement(28912..29805)
                     /locus_tag="VLD60_13315"
     CDS             complement(28912..29805)
                     /locus_tag="VLD60_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSB45987.1"
                     /translation="MPTLMSFWRLLLDLAKSFLRHGCASLAASLAFFSLLSLFPLVFL
                     LLYAVGFVVSQDVIGGQVLLSFLKGFLPTLGERLLVELQRVSEMETVRWFVFLSFVWF
                     GTLVFYELEYALNVVFDSTWKRHPLISTAIAVASQGAIGLLLVLSYVATQMVNLLIAY
                     VPRLWGLDLIALAAHDLFLTYTLPFTMAFLSVGILYRYGPRRRPQWREALIGSLTFSL
                     LWVAAKLLFVTYNEYATIYTRLYGSLLEVILLLLWIYYSAALFLFGAVIAHALQQRAQ
                     VVPAGSLAQADISSPSVPARR"
     gene            complement(29873..>30679)
                     /locus_tag="VLD60_13320"
     CDS             complement(29873..>30679)
                     /locus_tag="VLD60_13320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSB45988.1"
                     /translation="FHMWTPDVYEGAPTSVTAFMAVASKAASFGAFLRVFVEGLGGLK
                     ADWSNLLLLICVVTLILGNLVAIVQTNIKRMLAYSSIAHAGYALIGLVAAGRSMGESG
                     GTSGLASVMLYLAFYAFMTFGAFAVIGMFRKGGLEGEEIEDFSGLAKRQPMAAFLMLV
                     FMVSLAGIPPTAGFIGKFYVFMAAVEAGLAWLAVVAMVFAAVSAYYYMRVVMVMYMRE
                     PDAAVVDPPRLVASPALSVVLACAVAGVIIFGLFPNPLVSLALQSVLSLK"
BASE COUNT         6731 a         8292 c         9189 g         6467 t
ORIGIN      
        1 gagcttaaga agcaatgggg taagtttacc gatgacgatc tgatgcagat cgaaggcgac
       61 tacgacaagt tcatcggctc attgcagaaa cggtacggag atcagaaaga ggaagtccgt
      121 cgttgggtcg aacagtggta ccaaaggcgg gaggggtccg ggaagccagg cacgatgccg
      181 aaggcgggat aggagcgtcg aagacgcgcg gacggcggtg cttcaccata atcattgttt
      241 cgattgttca attcacacaa aggaggtttt ggtcatgaag aaggtattca tcgcatccgt
      301 gttgatggca ggtctgatgg gctcctctgc ctgggctctg ttcgagacga accaggaact
      361 tgcgaagagc gcgaccgtga cgctcgacaa ggccgtcctc gcggcggtag cggctatgcc
      421 gggaaaggcg gtcgaggcgg agctcagcaa ggaggaagga cggaccgtgt acaaagtcga
      481 gattatcgac cacaacaaca agacgcagaa ggtctacgtc gatgcccaga ccatgcaaac
      541 caggattgat aagtaggaat gtccgtgcat cacgccgaac ggcattccgc tcggcctgat
      601 cgctctggct actgagccca tacaaacagg agactcacga tgaaactgac gaacatgatc
      661 ctgtccgcag ccgtcgcagt tggtatgacc tcgaccggca cgtggtggtc ggcggaagcg
      721 gcataccaga ctcctcagcc accggcatcg gtcgagactt ccccgatcct gctggtcatg
      781 aacaaagacc aattccaggg aaagtggaaa cagttcaaag gggatttgaa aaagcaatgg
      841 ggcgagttca ccgacgatga cctcaagcag atcgaaggca attacgacaa gttcgagggt
      901 aaggtgcaag agcggtatgg ggaccgaaaa gaagatgtca agcgctggac ggatgagtgg
      961 ttcaagaaac agagcggttc caagcgaaac taggcaaaga gaacaaagag gatcgctgag
     1021 gagtctttca tgttctctgc ccgctcaacc aagactctcg attatcgagg taaagaaggg
     1081 agtggtccga tgctgtacta cgcgcttcta ttcctcatcg tggggatcgt ggcagccgta
     1141 ctcggcgcgt ccggcgtggc cgctgtggcc acgcagattt catgggtcct attcgttgtc
     1201 ggcatggtcc tgctgctcat tcacctgttg agcggacgga ctccgcccgt gacctgagca
     1261 agcgtccatc acacacagca aacacagcaa actcacgcgc tctagggagg agagtcatca
     1321 tgagacagaa ttgcatcata cgagaagtat tctcctggtt ggtgatcgcg ggattaggcc
     1381 tgagcctaac ggcctgtaac acgacgaagg cgaccatgga cacgacggtt aacttctttt
     1441 cgagcacgag cccgggctcc atgttcacgg ccgatgggat ggtggagcag aagcaaaaag
     1501 tgaatctgtt tgccggcgtc gccttcgaga acctgcgaca ggatatggcc caaggcggag
     1561 gtccttacct cacctctctt gcggttctga tgaatatccc gtccgatcgt caagacgagt
     1621 ttgcgcggtt cgttcaggac cggtatcccg tgttgttcac gtccgatctg gcgaccgatc
     1681 ataccgctca tctcaaaatg ctcgccgtcc tcgatcgtga attggagacg tatgaacgag
     1741 cgagataagc ggacgacctt tcatgggatg gctgctgagg ttgccgagac tgagaggcga
     1801 aggttatggg gccgttaggc aaaggattca tcttgtggat catgcgcata ccggccggcc
     1861 tcgtcgttct cttatggctg ttcggattct tgaagtgacg cttatgcagg atcaggaacg
     1921 attggcaaca gccggtttat gcctgatcga ccgatgaggc cgtagtccga gtgtaaggag
     1981 gcacccgatt gtgacttatg aatcggcatc tccaggccag aggacgtgag aggaggcggg
     2041 ccatgcgaac tatcatggac attagaattc caatcagaat aacggcgtgg ttgtttgtgg
     2101 tactcacgtt tgccttatgc atgtcgccgt tggagagccg cgcctcgttg gttccttctg
     2161 ttccttccga tggttcggta gtgtctatac ccgaatccgg aatggaccga gtcgaggatc
     2221 ttcggacgat tcagcatgtg ttggaaacca aggttgtcca gcaacgtctt agggatctcg
     2281 ggctcagtcg tgaagagatc gattcacggc tggatcgcct gtcggacgct caattacacc
     2341 aagtggcgac gcagattaac tcattattgc ctggcggtgg tattgacggg gcgttgagta
     2401 ccattttgac ggtgctgctc atcattctcg ttgcagtggt tatcctcatc ctcatatgag
     2461 aacacacatt cacataggac ttgcgtgcgt ggccttcgcc gcatgggggg cgactgtcac
     2521 cggctgtgcg ggatctccag gtgtttccac agatgcactc catcctaagg tgcaccacct
     2581 ggtcggggtg ccattctttc ctgatagcac cgaccagtgc ggcccggcga cgcttgccag
     2641 cgtcctgacg ttctgggggg ccccgaccga tccgcgttcg ctccgagaag aaatttatct
     2701 caatcatttg cggggaaccc tgccactgga tttgttgttg gccgcggaag cgcgtggctt
     2761 caaggcggaa gcctatctgg gcagtactga caatctccgt gaagaacttg ccgccggcca
     2821 tccggtgata gcatttctcg atcttgggtt tctgatgttt cctcagggcc attatgtggt
     2881 cgttacggga tacgatgcgc agcgacaagg tttctacgtt cattcaggac tcgaacgaga
     2941 tgccttcctg tcggacagcc ggtttgtgaa gagttgggac aaaaccagcc ggtggacgct
     3001 tcgagtcttg ccccaatgaa gagacgggga ggcacgcatg agtagactcg ggcggcatat
     3061 gtatatgagg agcggcatat gcgcgatgat tcttgccggc gcggcctccg cctgctccgc
     3121 ccagcgagcg atcatgccgg ctgatccgct tacagcggag gaacatgtca ggctgggatt
     3181 catctatgaa acgcagcatc tgaacgatct tgcagatcag gaattccagg caacactgcg
     3241 acagcgacct cgctcaacga cagcgctggt gggattgggc aatctcgcat tcgaacgtgg
     3301 agcgttcggt gaagcgcaag actattacaa ccgggcgctg gcaattgagc cggaccatcc
     3361 cggcgcaaac aataatctgg cgatgattta tctgacgcta gggagccggg tggccgaagc
     3421 cgaacgcttg gcattgacag cactgaaccg cgccggcccc ctgcggccct acgtcctcga
     3481 tacgctagca ggcatttatc gccgtcaagg tcgtgaacgt gacgcacaag acgcgctgaa
     3541 ggaggccgaa gccttgatgc cgggggactc cacggtcctt cggaatcaaa tcgacagtcg
     3601 cgaaccaaac tccaccgttc gctcccaaga gggtgccgat gcagtacagc cacgctaagc
     3661 tttcgcaagg atggatgcga atgaattgcg attaattcaa aggtcaatgg agtcagttca
     3721 aggaagagct caaggaggag cggggcgagt ttaccgacga cgggctgatg cagatcggca
     3781 cggcatgccc acaaaatatc acttccgtcg gatcatgccg gcaatggagg gcatccgtcc
     3841 tctgatcgag gggacgcgat gggtgcggat cgaacgatcg agaggaggcc catgaagaag
     3901 aaattcaaca agacattccc aaacgatgtg atggccgtac gtcggcgagc ccgtgaacat
     3961 accgacggcg gggcggtgac ggaagggtac cgagcggacc gtaagatggt cctcaagctg
     4021 ttgaacgaag ctctcgccac cgagttggtc tgcgtgctgc ggtacaagcg gcattacttc
     4081 atggcggatg gcatctatgc aggaccggtt gcccaggagt tcctcgccca tgccgcggac
     4141 gagcaggcgc acgccgatca aatcgcggcc cgcatcatcc aactcggcgg cgagcccgat
     4201 ttcaacccgg aagggctgct cacgaggagc cattccgagt atgtggaagc cgagtcattg
     4261 gtggagatga tcaaggaaaa tctggtggcg gagcgggtcg ccatcgattc ctatgcgggg
     4321 atgatccgct acctgggcga cgacgacccg acgacccggc gcatggctga agatatcctg
     4381 gcgaaagagg aggagcacgc cgaggatctc gcgagtttcc tggcaacgct cgatgtgtcc
     4441 aagcgctaag ggtgttccac agagccggtt atgccggatc gcccaagcac tttggacgat
     4501 ccggttccct gcgaccatgg tgcctgcggc agattagacc gaggagtgcg gaggttcagc
     4561 cgtcgagttt cggctcaaca tccatcgtgg gtggctcgca gtgctggtca cggacctgct
     4621 gtccgtcatc cgcatgtcgt cgtgacatgc agcctgcgta ggactggggg aggtgccgtt
     4681 cgagcaagca ggagggcttt atgagttcga attgccggcc gttcacaatc atgctgccga
     4741 tctccttcct tgtgggaggc ctggccgggc tggatgcgat accaccgacc gtcgaagggc
     4801 ccgcgggaag gatcacgaat gcggtggcca tgacacacac gaacaccacc gtcctcgttc
     4861 ttgtggcatc tcgctccggc ggaaagacgg agatcgacca gacgaaggcc gacgcctata
     4921 cgcgtcttga actggacttc cgtcgcgact atgagtcgaa gtacgccgac agcggctatg
     4981 cctactaaca gtaccgcccg gcctatcgat acggatacga cctcggaacc gacccgcgct
     5041 atcgcggtat ggactggaac gcggttgaac agcaggcgca tcgcggctgg gaagaaggca
     5101 ccatgggtct gtgggatcaa tataaagaag cggtccatta tggatgggat cacggcagcc
     5161 agagctaggt cgagattgat ggatcgagat cgccggaatc ttgcatgacc gatctagtca
     5221 attttctgta cctcagatga tcaacaccct gtgcagcact tccggatttg ctgcgacggc
     5281 atgatcgaag gccgcccgtc caagttggaa tcgagaatca agacgatcgg acaagctgtc
     5341 tgcggaagaa caagttgtca gaagtctcga tcttcacctc gcctcttact tttctgccgt
     5401 agcgtccata ttgccgtaat cctgcatgtt tccgtttcct cggtgcaggg accacacttg
     5461 gtggctcgcc ggtccatgat ttgctttgtt tcccatctca taaggaaggt gtcatcacca
     5521 tttcacgagg aggtaatggt catgacgaag aaatcagatc gactgattcg catacaggat
     5581 gccgaagaac ttgttcgcga tttcattgaa gccgagatat cgcgtgcgga cgccgacgat
     5641 ttctacttct tgaatcaatc tgaggagtgg tcgcatgacc tacgagcgtc gcaacgggct
     5701 cttcccgatg ctggcgatgc cgcccttctt ggcgggaacg cggaagcagc ctgggacgag
     5761 gacgaaatcg gcaacgaagg cgtgggcggt tcgatcctcg agccggatca ggacctcatc
     5821 gaacaaatga atgaggtctt cgagttgacc tacgaggaca atggattgtt ttggttggtc
     5881 gaaaggtctg agtggaggga tgcgcatcat tggaactgag tccggtcgct gctgagaccc
     5941 tccaagaccg gacgtggctt ctttccaatt gatagactga gcccgacatt cagggcgacc
     6001 tccggcgggc gaaatgagca gccacacgaa ggaggatcat atgcctcatg acagtcggtc
     6061 gttcacacaa catgagaaag agtgcattgt tgcctgcttt gcgtgcgcgg agatctgtaa
     6121 tgcctgtgaa gacggcatga ttgggatgga acatgaatcg gacatgcagt tgacggcacg
     6181 gtgcatccga cagtgccgcg aatgcgccga ctcatgcttg ctcgcggctc aatgggtcag
     6241 ccgacaatcc cctttggctg aacgtgcgtg ccagttctgt gcagaggtat gcgacgtctg
     6301 cgccgaaacc tgcgaacgac acgcgccgca ccatgcgttg tgcgggccct gcgcgagaga
     6361 gtgtagacgg tgcgctgaat tgtgccgtgc catggcggca acggtcgcta catcatgatt
     6421 gagttggccg gcatcggagg agaaagatca tccatcatga tgggcaattt tggttccatc
     6481 gtgagggctg gccgcgtctg ctcggattcg gacaagggac aagaagtgcg ttccaaatga
     6541 ccatgtcttc cgatcttgtg aaatccacga ggcggcgcag cggacgaggg ccggcgtgtg
     6601 tgctgtcgac ggtggcactc gtgatgatga tggagccgca cgaaatcgaa gacccgaacc
     6661 cgacaccggg gcccacgcat ccgactccgc ctccatcgcc gagtccgact ccgggagggc
     6721 cggggtcaac ggtcccctcg ccgccaccgg tttcgcctca acacacacga tgaggtcacc
     6781 atgcctacct acaaaacacc atccgtcgag aagtcgactc acaatcggct gccacatcgc
     6841 aggcacatga caatgaaaat catcctctgt ctgactcttg ctccatggct gctgccgccg
     6901 ttcgcgggcg aagctaacac ctacgacgcg atgacgcaga gcgcgaaacc gtacgaagtg
     6961 acgggcacgt tgttggagct agatttgtcg aaggggaaag ggttgctgag aaccgaccta
     7021 gggaagcgca tctatcttga ggtgagaaaa cccgaattgt ttcagaacct gtcagtcggt
     7081 gatcggttga cgattcaatt gaacggggac ggccaggtgg acaaagtgtt gggcgtagag
     7141 gtccccgaac tttccatcac tggccgaccg gtcccgccgc aaccgtaaat aaggagatcc
     7201 tatgaaacag gcaggtacct atatctcgag accatttaat ctgcacggct tgagcggcat
     7261 ttcggatcgc acaatcgaca tgcacatgaa gctttacgaa ggctatgtga aagaaacgaa
     7321 ccatctgacc cagttgctgc aggacattct gaaagacgga cgtgtggatc aggaagagat
     7381 gccggcctat tcggaactca agcgccgcct gggcttcgaa tacaacggca tggtcttgca
     7441 cgaatattac tttgagaacc tcatgcgatc cgcatcgaac ggtccatccc gtaacgcgcc
     7501 tttccgcgtg gccgcggagg aaagctttgg gtcgtacgag acttggaaag ccgatttcat
     7561 cggagtcggc aagatgagag gtgtcggttg ggccatctgt tatcaacatc cacagaccgg
     7621 cagtctctcc aatcactgga tcaccctgca cgaaatcggt aatgtgacgg ggtatatgcc
     7681 cgcgctcgtt atggacgtct gggagcatgc ctttcttctg gattaccagc cgtccgagcg
     7741 gggggaatat atcgaggcct tcttcggcaa catcaactgg cgagcggtag atgaacgggt
     7801 gacgcgctca caggcggccg tcaccgcgca ttcggcctga acgaggtgta cctgtaagcc
     7861 taaaaggaag gccggaccta aggatttggg gtcaaacaaa gaactgacgg agcgtcatcg
     7921 ctttcagagg cgccagggtc atcaacaagc attgttggaa cgagattctt gaaaacgatt
     7981 gcggccaccc agggtgtgcc ggatctcggc aagctcgata tggccaaacg acatcgagcc
     8041 cctgaaaatt acctgcgata aggggtaacc gctttctcga tcggacaaac gagccatctt
     8101 catgatgtgt tcggttttct ggcacaggcc gttgggccgg ctgtgcaaag gcgcagcctg
     8161 catcatgcgg aagagcaaac ccgcctatat tcactgcacc agcagttctt tcgccaatcc
     8221 cttcttgcag caagaatgat cgcaccgtag aatgtcgcgc gcttattgtc gatccccctc
     8281 agccgcaggc gaaggtgcac gaagaaactg gaatatagct gaagccgatg ggggaccgct
     8341 taaaacaggt ttcgtgaaac agggttcgtg cactgaccat cggccgagag ccatcttctc
     8401 ttgtgaggag agtccatgca cacaatcata ctgctcaccg tttccaatat cttcatgacc
     8461 atggcctggt acgggcatct gaagtacaag gactcgccct tgtggatcgt gattcttgtg
     8521 agctggggca tcgccttcgt cgaatattgc tttcaggtgc cggccaatcg aatcgggcac
     8581 tacgaattca ccgccgccca gttgaaaacc atccaagagg tcattaccct cgtggtgttt
     8641 tgtattttct cggtgctgta cctcaaagag ccgctcaagt ggaattatct cgccgggttt
     8701 ggcttgatgg ttggagccgt gtttctcatc tttaaagaat ggtgaggtag ggagcagccc
     8761 gcccattctt cttgctcgcg gaacgcggca cacaagatct atcgtattgt gaaattgagg
     8821 gcgatgctcg ttcgacgcgc gcagggaaga atagacgggc tgctcccttg tcagaggaaa
     8881 taagcagaag gagcggaatc atggcgccga agaagaaaga gacaaccaag atgaaggatt
     8941 cagcaaaatc tgctgcggca ccagctagct cagggtcggc cgaagatttc gaaaagctcg
     9001 gcgtctttta cctcggccgc ccctatgatc tggcagccaa gcgggcgaag ccgggatggc
     9061 tattgtacga ctcgaaagat ttggtcactc acgcggtctg cgtcgggatg accggaagcg
     9121 gaaagaccgg cctctgcatc ggtcttctcg aagaagcggc gatcgacggc attcccgccc
     9181 tcatcatcga tcccaaaggg gatctcgccg atttgatgct gacctttccg cagctgcggg
     9241 gcgaagattt tgcaccatgg atcaatgaag acgatgcccg caagaaaggg ctttcaccgg
     9301 cggattatgc caagggacag gccgaactct ggaagaaggg cctgggcgat tgggggcaga
     9361 gcggggaacg gattcagaag ttgcgcgatg ccgccgatgt ggtcgtatat acgccgggca
     9421 gcaatgccgg cattccggtg tccattctca agtcgttcgc cgcaccatcc caagaaattg
     9481 tggacgacgc cgagatgctg cgcgagcgtc tcagcacgac ggtgaccagt ctgttgggac
     9541 tcatcggcgt cgaggctgat ccgatcaaga gccgggaaca cattctcctc tcgacgattc
     9601 ttgaccgcac ctggaaggcg ggacaaaatc tcgatctggc ttcgttgatt cagcagattc
     9661 agaccccgcc catcaaaaag atcggtgtgc tcgatctcga ctcgttctat ccgtcgaaag
     9721 atcgcttcgc gttggcaatg cagttgaaca acttgctgtc ggcgccgggt ttccagtcct
     9781 ggcttgaagg agaagccctc gacgtcggac agatgctgta ctcgcgtggg ggaaaaccgc
     9841 ggctcgcgat tttgtcaatc gcgcatctca acgacgccga gcgcatgttc tttgtcacgc
     9901 tcttgctcag tcaaacggtg ggatgggtgc gggcgcagtc cggtacgacc agccttcgcg
     9961 ccattctgta tatggatgaa atcttcggct atttcccgcc tgtcgctaat ccgccatcaa
    10021 aacaaccgct gctgacgttg ctgaagcagg cgcgcgcgtt cggtctgggc gtggtgctgg
    10081 cgacacagaa tccggtcgat ctcgattaca aaggcctggc caacaccggg acctggttta
    10141 tcgggcgtct gcaaaccgaa cgagataagg cccgtgtgtt ggaagggctc gaaggggccg
    10201 cggcgagcag cgggaagaag ttcgacaaac aggggatgga acagattttg gcaggcctcg
    10261 gtaatcgtgt gtttctgttg aacaatgtcc atgaagatgc accagaggtc tttcagacga
    10321 ggtggacgct ttcctatctg cggggtccgc ttacccgcac acagatcaag acgctgatgg
    10381 accccgtgaa acgtgaaacg ttaggcgtaa ggggtaagga ggagagtaca aagttgggat
    10441 cgttggaaat ttcgtctcac gtttcacgcc ttacgcctta cgcctcacgt ccgatgctgc
    10501 caccggatgt gccgcagcag ttcatgcctg tcaggggaag ccagccgagc ggcagtgcgt
    10561 tgatctacca gccgatgtta ctgggcgctt cccaagtgcg tgtgtccgac agcaaggcgg
    10621 gtatcgatgt gacacaggat gtgaccgtgc ttgtcccaat ttcagatggc gcagtggccg
    10681 tggactggga ccatgctgcg gaagcggccg tggctctcgg tgacttggag tcgtcacccg
    10741 tcgagggtgc gcagtttgcc cctgtgccca gcgcggcaag caaagccaag agctatgagg
    10801 tttggagcaa agatttttcg agctggctgt tccgaaccca gaaggtcgat ttgctgaaga
    10861 gccccagtac gaaagtgatt tcgaaacccg gcgagtcgga gcgggatttc cgcgtgcgat
    10921 tgcagcaggg tggccgtgaa cagcgggaca aacagtcaga gggcctgcgt caaaagtatg
    10981 cgccgaagat cgcgtcgctt caagatcgca tcagacgggc cgagcagatg gtcgaacgtc
    11041 agcaggtgga gtcccgatcg agtcaagtgc aggcggctat ttccgtcggc gcgacgattc
    11101 tcggggcatt cttgggacgc aaggcgatca gcgcgacaaa tatcggcaag gcgacgacgg
    11161 ccattcggag cgcaggacgt gtgatgaaag aaactcaaga cgtcggccag gcccaggaga
    11221 atgtggctgt actccaacag caactcgccg atctggaggc gcagttcaaa gcggaagccg
    11281 atgcccttgc agcgacaacc gatccgctca acgaaaaact cgaagccatt tctctcaaac
    11341 ctgccaaggc caacattgcc gtgaaactgg ttgccctggc ctggacaccg cattggcagg
    11401 atgcgaaggg cacgatgagt tccgcctggc tgtagcgtag cgtcgagaaa tcgcggcatc
    11461 gatattcagg cacgcggatt ctcactgcgg tcgcgcagtg tttcaaacac cttactgagt
    11521 gcaggttgtg aggtcgcgag tgccgtggtg aaggcttctt cgacaatgtg ttccccaagc
    11581 cgccggtagg attcgaattg ggactcgtcg aagaactggt ccgtcgtggg ttcgtgcgga
    11641 aagcattcat ggttgctcgc atattccaga acgtccaccg gttcttctcc ggtaagcgag
    11701 ggtttcagat agatgaggat gccatcctcc gccccgggat ccacacggct atagtggatc
    11761 gtgccgatcc catggtgtcc tgaggtgtgt ctggtcgact cttgccgttt cagcctttcc
    11821 agcttgatat caatatcgac gcccagatca atgcggattt tccggatcgc atttcccaga
    11881 tcttgaaagg cgaaatccgg atcggcgcct gcgtcgctca caagtatgca atgacatcgg
    11941 cgcagcacca tctcgtacag acccagatta tcgaagtgcc ctccgtccga caggtagaca
    12001 taccggctat tctcgtccgt cagtccgaag gcctctgcga gcaacgggcc cacagaaaat
    12061 tcggggcaag accggtcgta ggttcgcgcg ccggcgtcgc caggattacc cagccaccaa
    12121 cccagccgca cgttaaagaa cgcgagcaag aaggcgacgg caggcgatga atgatatccc
    12181 atattggggc tcgcagccgc tccggagatg gccatggctg ttccgaccgt aatagccttt
    12241 ccgaccccac tattggtccc gtaccgggaa gccgagcgat agcccaagtg actcgcaaag
    12301 ctgccgcaat gcagcggtga gacagtgaac gactgtgctt ttcgctgctg ccacgcgaga
    12361 ttggtgcctc gtaccaggtt gagcgcgatg ttgatgacgt gaaagggctt cgagattggt
    12421 tggccaggtg gcgccagatc atgcatctgt atattgtcgt cgggatcgaa gccggtgaag
    12481 aggttcgggg tccgtttcac gccatacttc tctaattggg aggcgccaag atacgcgagg
    12541 atcagacggt tgcgatacat agagtggagc gaaaacttat taatattaat cacccaggcc
    12601 atgctgcagc cgagggctgt cagggctaag ctcgtttcga ccagcaacca gaagggggca
    12661 ttgtgaatga cgttcgcatg gccccacggg tcactcagca tctgcaggcc gatggactgc
    12721 aggtcaaccg ctaaaactcc gtgcaggtgg gccaagatct tcaggagcca actcgtaccg
    12781 agtgccagca ccgtgagcaa gaacacgata aagacagggg ttgccagctt gactgcgtac
    12841 tcggccactt tcaccgatcg cctgtttctt tggctcgtgg tgggagcggt cctcgagctg
    12901 aacccaagca caagggtgat caggccagag aatcccccaa gtgaggtgat cacggcaggc
    12961 gtgaaggcca gaatgccggg tccgaacatc actaacgtcg cggccgcaac ccacacgact
    13021 cctgccagta acacccacga gccggatcgc gcccaccatt cacggtcatc atcgtcagtg
    13081 tatcggctag cgacgccgac gaacagggtt gcggtgagaa gaaacacggc caggactccg
    13141 ggaaccgcga acccggcgta ccactcggca aagctcgaga ccggcatctc cgacggcgtt
    13201 tccgccagta ccgaccagag gaagaatccg cccagcgcac cggtgccggc gatcaccagt
    13261 atttcaacga acagccagcg gctcagatgc ttgaatcgac gtaacaacag cgctgagagc
    13321 agccagctaa tcaaatgaat cagaagggct cccaccatga aggtttctcg actgccgagc
    13381 ccatcgacgg ctagttgatg caacgtgcca ccggcgttgc gaaaccaggc ccatgcggtt
    13441 gtgagcagca gaacactcag caatgtgggg atgagagaaa gagtcaggaa ccacttctgg
    13501 ggttcaaacg gtttccattt ctggcgttcg atcggtttct tgagattttc tgggcggtac
    13561 ggttcaagac tcggcctata gaggtgaagg tagctcatgg cgacaacgat aagacccaga
    13621 ccggcgtaga agagaacctt catgagtgat tcaggcagac ccatgtccga aaggttccac
    13681 cggacaaggg cgaccataaa ccttggtacc atcaatccga caaccagaag agggagcagg
    13741 accagccagt tgaggagcag gttgcgtagg tatgtcccga tcagtgtcca ggagtctaca
    13801 gagagcagcc ccaaacgtgg gctgagaaaa ttgctgtacc tgcggagcca tgtcaccgga
    13861 agaggctccg tgtccaaagg cgtctgtggc tgccgtctaa gttcttcggc tacacccgcg
    13921 atccgttgtg ggtgccgctt gatccatgcc gaaagccaac tcccgatgta gccgcctccc
    13981 gagacagtag aaagataggt aaaccgctca agaaggttgg tgcgggccag cccctgaagc
    14041 acaccaagac cgaaggtcgc actacgaatg ccgccaccgg agaaacagag ggcactgcgc
    14101 cggtcgggaa gaccgtggat gagctcgtag actgctttca ggcgctcgtc ctccgatccg
    14161 ttcacggggt agtcctcaaa gctggggtgc ggaagctttt tgtattcaga ctttagcgta
    14221 gccgtggaat cggctgccgg ggaggtgtgg agcttcaagt actcctcttc caggatcgtc
    14281 ggaagccgga gtggggcatg cgggccttgt ggggagggca tgacgtacct cctacgactg
    14341 tggggtttcg agccacttca cgattctggg tcggtacttg tcgacgacct tctgggctgc
    14401 cttgacgagc agcgccccat gacacggagt tttgatctta aacagggttg gaatctggcc
    14461 gaccgcctgc ttgatctgct ttggcgtcac attctgagag aattcgcccc gacaagcctc
    14521 gggcaagtct tgatagccgg tccagtcggc gtcggtgtgt ttgaggacca caatcctcag
    14581 atgggcgaag tcaggcagga cgacaccctc ggtcctgaga ctatgccaga gcagggtttg
    14641 atagacgttg gctcgttctt ccatgatgcc gacgatgcgg gagggatcgt ccttgaacac
    14701 ttcgaagccc ttttcgctgt tgtccaattc acccgcattg gcgtcgaagg ggaaggaggt
    14761 gtcaatgacg atgatcaatc ctttcttgga agagccctgg gggatcttct tcaaaaacag
    14821 agtcgtgagg gattccgttc ccaggttatc gaataaccca ccgtcgccga tgtgatgata
    14881 tggcggccta ccgtccacgt tataggtcac tggtccaacc acgggtggaa aggaggccga
    14941 ggtcgcgact gccagagaga cccgcagatt ctcatgatta gccccgattt ggtcgaacgt
    15001 gagtggaagg aattgctttt tggctttctc cagcccgctt tgaatgatcg ccaagccttc
    15061 gggtgtgacc ggtaccggtc gatcgggctt cttcaactcc ttaatcagca gctcgatgaa
    15121 atcatactcg aactcctcgg aaggcaacgt ggtgagcgca aagcgctggc cggagttgta
    15181 caccgttccg ttcaggatta cccgtgggct gtcgccgcgc tgttcccgtg catagagctg
    15241 ggagaaggtc atctcgtgaa aaaaattgct gtcccagact tcagaaaatg aataggcgaa
    15301 tttggtgggg ttgaacgctc tgaagaacag cagttgccgg acagctgcgc gagattggaa
    15361 atccatctgc atagccgttt tgaagtcgga aaagaaattc cggtacaccg gcgagagtcc
    15421 ctgatcaccg agcaccggaa cagacttcgg gggcttcgtc atcgtgtagt aggccgtagc
    15481 caggctgcct ccggagacgc tggacatgtg ggtcaccgtt tccaacgcgc tgcgttcctt
    15541 tccatcgcgc gtgatgcgga tctcggccag ggcttccagc gcgccggcgg cgaatgttgc
    15601 agctctgctg cctcccccgg atacggccaa tccaacgagg gtttcctggt cggccagcgg
    15661 cgcgaatacc ggggtattcc tgtcgggatg cgcaggaagc tgctgaattt tcgcaaagca
    15721 acctgtgagt gtgagtgaga accccagtcc cagtgcgaac aacacgttca tgatggtggc
    15781 ctcctctgat gctcgatgat cagtccgttc gtggtatcac cgcgtcgcgg taaggaccgc
    15841 cagtgcatcg gtcttggtgt cagcatgatt catgactagt ggctttccct ttgtgacttg
    15901 gaaatagcag aaggcttcgt gctcggcgag ttttttggcg agaacgccac ccgctgtttc
    15961 cacggcggca gccaatgttt cgttgtttgt ctcgaacacc ccgcctcctc ggaccgcttt
    16021 gccgacagcc gatgtgacag ccgcttcagc gcgcatcgcg tcacgcgcca cgcgagcaaa
    16081 ctgctcgtat gaaatgggtg gatggtccgg gtcaatacgc gggtaggggc gttcatcccg
    16141 gctccactta accttgagtg cagtggcttc ctgggtggcc gggactcgaa aggcggcgtc
    16201 gaaaagggcc tctaaaaaga tcctcaccaa atcggatgcc acatgtcgtg agtcgaccga
    16261 gctcgcgtgg aaacgaacgg tcggctcctg cttcacaggg tccatcgaga cggtgacctc
    16321 tgaagaaagc ccgaggaaag caaatgcatt accgttgcgg tcgacgaacc cggctgacac
    16381 caccttaaac actccttgga ttccggatcc tgtctgggtg ggctgagtgc ggaattgaat
    16441 atctccgaaa taggccttgt tataggcgag gaggagttgg gcgaataggg atttgtcctg
    16501 agccaatcgg tcttcgtcga tccggaatcg agcgtggagg cgtttcccca tttcggcgat
    16561 gcggatgaga cggccgatgt cgaccttcct ctcagtcgga tcatccatgt cacgaattaa
    16621 gtccagcagt ccgtggatgt cctcatcggt ctttagttga ggactgtcgg ccactgctgc
    16681 ttcgactgca ggcaaggccg gtgctacgga cggagcttgc ccaccggggg ttatcgaggg
    16741 agtggcagac gcactttcag ctgtttggtc cccataggcc tgccgactgc agctcagaaa
    16801 ctgctttgca agctcggcgt tccccttgac cttctcagac agtccggatt gctcgcccag
    16861 ctgcaggatg ggtgaggcgc aaccggccag aacaatgagt gtcgcaacgg cgattcgagg
    16921 caagacattc caacattgca taaatcaccc ctcaattggg cgaagacaga aatgtcacct
    16981 gaccccctgt ggccgaagta tgaaatgtga gagcaactcg tatgccgata atgattgcgc
    17041 aggatcaata ggagaagtca gtctacccat ggtccatgcg ggaggtggaa ctgaggccga
    17101 ttgatcaaat gagctacaag ttgtatcgat tccaatacga gctcagtgtt tcttctcctt
    17161 ctgcatgatc ttgtcggtcc aggccagcag gatagccgag ccgaggaaag tcatgtggat
    17221 gaagatcttc cacttgacgt gttcgtaatc ttcattggcg atgtcgacga acgatttgag
    17281 caggtgaatg ctggagatgc cgatcaacga ggctgcgagc ttaatcttga tggtgccggg
    17341 atcgacgtgc gtcagccaat ctgggcggtc cgggtgagtt tcgaggtcaa gtttgctgac
    17401 aaatgtcgca tatcccccga tgacgaccat agtcaataag ttggccacca tggtcacatc
    17461 gatcaagccg agcacgccca acatgaactt ggtttcttcc aactgttgaa tgtggatagc
    17521 catttcccaa agttcgatca ggaatttgta ggcgtagagc agttcggcga cgatgaggcc
    17581 ggcatagagc ggagcttgaa tccaccggct ggcgaacaca accgtttcga gcacgttttc
    17641 aatcggatgg tcggcggctt gcgagtttga agctggaagt tgatcggaca tgccactcct
    17701 cggtgattga accttcgcgt ttgtctgaaa gataccccct tacgagcgcg ttatcctaga
    17761 taggagaccc ctctgtgtca aatccgatat gaattgaccg agtcagtcgg gcagggcctc
    17821 ggcagggtcg gacagatcct gttccggttc ggggaggcag agcagccgaa cggtgttgac
    17881 gactttgcgg tcttcgagcg ctttatcgaa taaaaagtcc gcgccggcga cggcacaagc
    17941 cgatcgcaac tcctgcgaaa gttgatttgt taccatgatg acggtgggat gaagaatgag
    18001 acgcgtgatg tggcgaagga cttcgatccc gcttccgttc ttgagatcaa tgttgagaat
    18061 gatgacgtcg ggtttgcgat tgacaatcat tacagtggtc gcttcggctt cggccgtttc
    18121 tccgatgacc gtaacaccgg agatcttctc caagcgacgt ttgaggcgtt gacgaaaaag
    18181 cgggtcttga tcggcaatca gaatattcat cggcgatagg aggaaccctg aaagggacag
    18241 attgataact gacagcagta tagacaacct ttgtgtatct attgtcagca tcgatccgtc
    18301 atgtaccgta ggatgtttct ggcataaatg gatgtattct tcgattcgat ggcgcgatgg
    18361 catgcgcggg caggcatatg ctcaggaaaa gccgcgggaa gcattcagat gggcatggcg
    18421 gtagcccaaa gagaccatcc gctcatgacg aggggagaga gagggtgatt gtcgttccct
    18481 tgttcagtgc cgatttgatg gtcaagcgag cccgaagtct gcgagccctg gcccgcatgt
    18541 tcttcaaccc catgccttgg ttgtgccggc gagctgtctc aaacccgtgg ccgttgtcgg
    18601 tcacctccaa ccgaattttt cccctctcct gccggagcgt cactgagcgt cgcgtggcct
    18661 tagcatggcg aacgctatta ctgagcgcct ccttggtgat cgcgaagaca tgcaggcttc
    18721 gttcaggaga cagtcgcccg gcggcatgat catcaatgga caatgagaca ggacctgctc
    18781 ctgttgccat aaaggaatgg accagcgacc gaattgcatc ggccacggtt ccttcctgaa
    18841 tgcgtggggg ctgcacgcga gggataaaat tgcgcacctc gcgaatgacg ccgttgagct
    18901 ggtcgatggt ttcctcaatc cgctttgcgt tcgtttgaga gaggcgctga gttttctgtt
    18961 tggtcagctc cagccccatg ccgacggcgt agagcgactg gagcaggttg tcgtgcagat
    19021 cctggctgag acgttcccgt tcctcgaatg ccaggcggac ttcctgctca ctgcggcgca
    19081 atgcctcttc agcacgtttt tgttctgtta tgtcggttcc gactgtcacc acacactggt
    19141 tcccgttaag ttcgatcaat tcgcacgaaa ccaagcagcg ccgtgcagtg cgatctttcg
    19201 tatagaacgt caggtcgaga ttgcggagcg aaccgtcggc tttcaagcga tcgatgaatc
    19261 tctttcggtc cgcatcggtc ggccagagtc ctaaggtgat cgtgctctgt ccggcaactt
    19321 cttcccggcg aaaaccgaac aagcggagcg aggcttcatt cacttcgagg caacatccgg
    19381 tttcgagttc ggtcacgatg atcggatgag gacttgagcg gaaggctttt gcgaaccgtt
    19441 cttcgctgac tcgtagcgcc tcctcagcct gtttgcgttc ggtgatgtcc aaggcaacgc
    19501 ccacataatg agtgacacaa cctgtttcac gatcccgaac aggaaatccc cggtcccaca
    19561 cccaccggac catgccgtcc ggccgtacca cccggtattc gtgggcaaac ggcaggccgt
    19621 cctgctgtac ggcaagacca gcatggacat gcgcacggtc ttcagggtgg atggactcga
    19681 ggaaggactt cgggtgttca tacagactgg agcaggtgcg tccccacacc cgctcataag
    19741 ccgggctgat atacagcatc ctgccgattg caggatcggc agaccagatg acttcttcga
    19801 tggtctcggc aatctggcgg aaacgggttt cactcttctg tagcgcttct tcggctcgtt
    19861 tccgctctgt gatatcgatc caggaaccga taatttccag aggattgccg gcctgatcgt
    19921 tcagcagttt taagcgatca tgcatccagc gatatgtccc gtccttgtgc aagaagcggt
    19981 attcgtcgac atggtgtcca tctcggaaca ggcatgagag attggccagc acacgaggct
    20041 tgtcgtcggg atggatatga gtggcccaga aacccgaatc gtccacgaac tcgcgcggct
    20101 cataccccag ttgctcgaga atgttctcgc tgacataggt cgcgccgtaa tctcctgatg
    20161 tcttggcgga ataaatgact gtcgggctgg atgccataag gtgctggagc cgttccctga
    20221 tcatgcgcag ttcttgctcg gctcccttgc gctcagtaat gttctcaacc atcgcaatat
    20281 gcagccggcg gtctgtggca tcgagccaca ggggcacgca tgtcaggttc acccacacgg
    20341 tcgacccgtc ttttcggatg tagcgtttct ccatctggaa cgtgtgtatt tcgccgtcca
    20401 agagccgcgc catgttattg acgtcggctt cgacgtcgtc ggggtgtgtg atcatctgaa
    20461 acgtgcgcgc gagcatttct tcttccgagt agccggtgat ctcgcagtac ttgttgttga
    20521 tctgctgaaa gtgaccggtg accgaatcga ggatcccgat gccgagcggc gcttgctcga
    20581 ataaagctcg agctctctgc tcgctctctc gcagggcatc gatggcccgc ttacgctcag
    20641 tcatatcggc caggaatccc agtgtgccag tcacgatacc ctgagggtct ttgagtatcg
    20701 ttgcccagag ggcaaggtcg atgggtgacc cgtcgcgacg tgtgcgcctg agttcaaccg
    20761 cggagaggga accctcttga agaagcctcg cccataggcg atctgactcc tctcgcttgt
    20821 cggaagggag aaacggtgca ggatgtccta ttacctcgtc tgcgcgccat ccaaacattg
    20881 attctgccgc ctggttccac agtgtgacgg tttctcctgt ggtatcaagc gccatcacag
    20941 caagcggtga catatgaacg agcgcttgga gcgcctgaac ggtgatgcgg ttttcctctt
    21001 ctgctgcttt ctgggctgtg atgtcctcgc agatcacgag gacgagaagg gagccgttct
    21061 cgtctcgaac ggctcgggcc gtttctctga cccacaggcg agtcccgtcg cgacgaactt
    21121 ttttaatatt ccattggaaa atcttgtacg gatgagaggt acaggctcga agttgctcgc
    21181 gaactgtctg atggtcggcg ggatcgaaca cgaccagcac ggattggcct gtcagggtgt
    21241 cagccgaata tcccagttgg tcggctccga atcggttgac ggacaggacc gtgccatctg
    21301 gagaaagggc aaacgacatg gacggggtat cttcatacag gagtcggtag tggtccgatg
    21361 agggctgagt ggtgatcccc gtcggcggcg ggtccgatgt gcgctggcct tcaagttccg
    21421 ctacacgctg tcgaagttgc tggagttcct ccacgagagc cggcgaaacc tcaagcggtg
    21481 cgctcataat cctctcctcg ggtgtagagg gcggcagggc ccctagcata ggatgtccta
    21541 gggctgaaaa gctaggggga tatctgacag gtcgtaaggg aagttaagta gcgggaggct
    21601 gttcgacggc cggattagtc aggcaaaatt tggctggagg gagcggttcc ggcgactgtc
    21661 cgaagttcac gaatggtctg tgcgctcagc cctgcctcct tcaactcgga gtccgaggct
    21721 gccaggatcc cctcaagggt tttgaaccgg ttcaggaggg tggtcctgcg gatctccccg
    21781 ataccgatga cttggtccag gcgggaagtc atgagcgcgc ggctccgcag cttccggtga
    21841 aacgtgacgg caaaccgatg ggcctcgtcg cggacacgtt gcagcaggtg agtgccaggt
    21901 gacgtcggtt tcaggaggag cgggttcttt cggcctgcca aaaaaatccg ttcatccttt
    21961 tcaccgcggg ccttggccag acccaaaatg ggaagtccct gcgcgccaac ctgtttgagc
    22021 ccctccatcg cggcggccaa ttggcccggc cccccgtcga tcaatacgag atccggccgc
    22081 gcgaggtctt ccactgtgcc gtaccgtcgc gagacgactt cctgcatgct ggcaaaatcg
    22141 tttgcgccca cgacggtctc gatcttgaac cgccgatagt ccgccttctt catccggcca
    22201 tcttcccaca cgaccatcga ggcgacggat tgattcccca tcgtattgga aatatcaaag
    22261 ccttcgatcc ggtgcggaat cctttcaaga cggagcagcc gttttagttc ctcgccagcc
    22321 tgccggtcga gcgcttcatt gcgcaaatgg tcggccactg cggccgcggc gttttcttcc
    22381 gccaagagga cgagttggtg tttgacgcct cgttcgggcg caagcacgcg caccgtttcc
    22441 ccgcgcttgt ctgagagcca gcacccgatc aattctgcgt ccgggatgtc gacaggaatg
    22501 aggagttcgc gtggcggctg gccttccttg ttgtagaact gttcaatcgc cgaccggacg
    22561 agttcttcat ccgtggcatc ggcggattgg ggccagaaga aatctttccg tccgatcagc
    22621 aggccgccgc gaacaaacaa caactggaga tctacggccg cgccttgtcg ggcgataccg
    22681 acgacatcct gatccacgga agccgtttgg gtaatcctct ggcgttcgag cgtgcgctcg
    22741 atattaaaca tgcggtccct tagtctggcc gcttcttcga aattctcccc ttccgccgcg
    22801 atttccatct gggcgcgcag ttcatccaag agctcccggt cgcggccttc gagaaaatgg
    22861 cgtacctgct gcacaatgcg atggtagtcc tctttcgatt ggttgcctgt gcaaggggcc
    22921 atgcaacgct tgatctcgaa ttcaatacac gcgcgctcgg ccttgccgtc gatgtcgatc
    22981 gtgcaggtag ccaatgggaa cacttttttg atgactttca gcgtatcgcg cagcgcaccg
    23041 gctggagtgt aggggccata gtagagagcg ccgtcctttt ggacgcggcg cacgatggag
    23101 aggcgaggga actggtcctt gatcggcagg cgcaaatagg ggtactgctt gtcgtcacgc
    23161 aagacgacat taaagcgggg gcgatggcgc ttgacgagat tgctttccag gatcagggcc
    23221 tccagctcgg accgcgtgac catcgtttcc agatcggcga tttgactaac catcagggag
    23281 gttttcggag agtggtcggc acgcttgagg aaatatgacc gcacacggtc ggccagtctg
    23341 gccgctttcc cgacatagag cagttccccg gtgctgtctt taaaaagata gacgcccggc
    23401 tgctcgggca ggtgagcgag tttggattga agcgtgtcag aagtagccat aaagctgtca
    23461 gccttcagcc cagggggtca gccaaagcaa tccgccggga tgatccaagc attctacggc
    23521 ctggaggctg ggtgggcaac ccggggctag gaattcgaaa gcggacagct gacggccgaa
    23581 cgctgatagc ttgctcagcg gagcccgcgc acgaaggatg gactgagtgc gcctcgcgcc
    23641 acttcagcga cggggccttt catggtgagg ctaaaatcct gagatacttc gatattaagg
    23701 gtgccccccg gcatcttcac ggtgacgggg ctcttgacca gcccgagccg cacggccgcg
    23761 ctggccgccg cgcaggagga ggaaccggag gcttgtgttt caccagcgcc tcgctcccag
    23821 atcaggatat aaatttcttt cggaccggtt gggacagcga gctgcacatt ggtacgtttt
    23881 gggaagagcg cgtgattctc caatgccggc cccagcgcca gaagctcctc gcgcgaccag
    23941 gaagcgccgg ccggtttgaa gatgacgcag tgggggttgc caacactgac gccggtgaat
    24001 gtcagcgatc ggccggctgc ttcaattggc tgctgcgtca gttcttctgc cggcaacgag
    24061 cagggcaacg ccgctggttt gaaggtagct ttacccattt caacggtcac ggcactggct
    24121 tcgccatggc gatcgacatg gagatcaatg gttaccaggc cgcctttcgt ctcgactgta
    24181 aagtgtcgct tcttggtctt gcctgtcgca tggaggtagc gggcgaagat gcgaagtccg
    24241 tttccggatt tttccgcctc gcttccgtcc ggattgaaaa tgcgaaggcc aaagtcagct
    24301 ttcttggaag gggccagggc gagaatgccg tcgctcccca gtccccagtt acgattgcag
    24361 atcaattgga cggttttggg cgtgagcttg aaactcagct ccttgggatc catcactatg
    24421 tagtcgttac ctaatccatg tccgcgaaaa aacccgttct tcatgccaga ctccgtgagg
    24481 gtgaagcgat tgtgccggcg gggaaggtag catgaagacg atcggtagga aaagagggtg
    24541 gagactagac gatcttgacg ccggccggcc gttcgatgag ctccacctca tacccgtccg
    24601 gtgcatcaat aaagatgaat cgactgcccg aggagctttt cgtcgggccg tcagtgatcc
    24661 tgacgccttt gctgttcaat gcggagatcg tctcgtcgag attatcgact tggaaggcaa
    24721 gatgcacgag gtcctcctga accttgacgg gaccgctggg aggaaagctg cagagttcga
    24781 tcagttcttc gctgttcggc acgctcaaga acgccaggtg agagcctctc ggcgaggtat
    24841 gacgctccac cacctccagc cccaatacct gcgtgtagaa ggcaatggtt tggtccatgt
    24901 cgctgactcg catgcgggtg tgcaggagtt ttttaacctt cagggtgccc attatgagct
    24961 ttcagcggtc ggcattcgag tctggaagct gacagctatg cttttctctc tcgtgccggc
    25021 cctaacgttt tccgcaacaa aatctcggcg ctgcccggga tcaagaagtt cggcatcggg
    25081 ccgatttcct ggtacccctg cttcttgtag aatgcacgcg cgccgctatt gaaatccgat
    25141 acgcaggcaa agagattctt agtccgtgca aagacctgcg actcgatgtg agcaagcagc
    25201 tcctttccaa tccctttgcc tctggcctgg tcagttacgc cgagcagttc cagatagtcg
    25261 ccaaggagaa acttctgacg aacaatcgcg actgcagcga tggttccgtc aacgaccgag
    25321 acaaaactgt cccgcccttg cgggatcggg cagaaaatcc gcccccagtc ttccaccgta
    25381 taaccgagtg atttccatgg gtcagattca gccaggagtt ggacgaccgc ctcgcaatct
    25441 cctggctgca tggctctgat gtcgtgacgg ttcattgatt tggttgcttg caggagagaa
    25501 gttcgctcac ggtcatcggt ttcagcctct tgcgcggaag cacctcggtt gtgagccgtt
    25561 gaatgacctg ccgagtctgc ttccccttgc cgttggcatg caagactaca atgcttcccg
    25621 gtctggctcg cttttcaatc cgtgccagga tttgatcggc tgaaagagtg ggatccggat
    25681 cgccggactc gatgttccat tgaataaact gaagtcctag ggatttgacc aggtcaacgg
    25741 tcacgccgtc atattcccca aagggcgggc gaaacaaccg tgcttcatgg gcatagtgct
    25801 cgctcagcat cgtgatcgga ccgagaattt ccttccgttg ctcctcggca ctatgcattg
    25861 ggaggtgggc atgaacctcg ccgtgcgtgc cgacttcgaa gaagccgatg ccaaggagct
    25921 gttcgacttc cggatcatgt ttggcgatcc attgccctga cataaagaat gtcgcgggga
    25981 tcttgtgttc ggtgaggtag tctaccaagg cttggtcgta ccccggactt tttcggaccg
    26041 ggcagagatc gaacgtcagt gcgatgccgg ggcaagcggg cggaccggac ttaatgactt
    26101 gcgcgttacc gggttccggt gtcatctgaa gcggcagcac ggtgagcagc aagatcagga
    26161 tcagctgcga gcgtcggcga gccatgccct ctagtatacc cgaagacttc tggagcaagt
    26221 cctgtaaaaa gtcagtcggt tagggggcgg ttgacgctcc gattcacgca ctgatacggt
    26281 gattcccatg aatgtgccgg cctgggaaat aggacgagcc ctggggattc cgatccgcgt
    26341 gcatccctcg tggtttctcg tgtttgtttt tatgacctgg accttggcaa ccggatattt
    26401 gcccgaggcc ttgcccggtc tgtccggctc gcgttactgg ggcatgggcg ctgtggctgc
    26461 gctgttgcta ttcctgtccg tcctcttgca cgagctaggg cattcgtacg tcgcgctgcg
    26521 ctatcgcatc ccgatcggcc agatcacgtt gttcctcttc ggcggtgtgg cccatatgaa
    26581 gaaggaaccg ccgagtccga gagccgaatt tctcattgcc atcgccgggc ccatcgtcag
    26641 ctttgcgctg ggggcgttct gtttaggcac aattgaggcg gtggatgcgt tgcccatgtt
    26701 ttctggcgga cgcggctttg tcgtgcttgg agggttgctg ggggtgaaca atattatgct
    26761 gggcctcttc aacctgatcc cgggattccc cctggacggc gggcgggcgt tgcgcgcagg
    26821 gctctgggcg tggagcaaga actttcatca agcgacgagt caagccgcct ttatcggact
    26881 ggggttcgga tttctgctcg gcggattcgg cgcgttgctt gtcagcgggg cgatttcggg
    26941 catgttcgat ccttcgttgg caagcagagg gggctggctc ttgtttatcg gagccttcct
    27001 ctttggtgcg gcctggagca ccaggcgtca ggtctctctt cgcgcagccc tctcttcgac
    27061 gtcagtccgc gacgtcatga ttcgtgcggt ggtgacggtc ccgcagcatc tgactcttca
    27121 agccgctgtc gacgagtttt tcgtcgccta tgggtatgga ggatttccag tgtcggatga
    27181 gggaaaggtc gttggcctca tcgcggtgga aaatgtgcaa gcggttccgc aaggactctg
    27241 ggcctggcgg acggtccgtg acgtcatgcg ccctgcttct ccgggcctgt tcatcccgcc
    27301 ggactggtcc attatgctgg ccatggaccg catggtacag gagggatggg atcggttagt
    27361 ggtcgtggaa gagggtcagc cggtcggtct catcacccgg tccgccatcg cgcagtttct
    27421 tcaactccat aaaccgtaag actcatgtag tgcgcgtggt tcccgacgcc tacccgtctg
    27481 agcgggcgac ggaaatgttt tgcgcgatgg cgatacgaac gaggtggacg acgaggagaa
    27541 acccgtagac gacgcagaag aacttgaagg tgacgcgata gaagtccatt aataggacgg
    27601 gaaggcagag agcaaagatg agactggcga tcagggtcat gaaggtcagg cggcggcaga
    27661 attcgtacga cgcttccttt cccaccggtt tcagggcgcg cgcgtaacgt tctgcccgcc
    27721 cgagccgcgc ttcttgcaac gttgaatcgt tcggccctct atagcgtaaa tacaaatatc
    27781 cgagcagaat caagcaccag ggcatcgccc agagaggcca atacgttgca cctccgtagg
    27841 tctcgctgct gacgagcgtt gcgaatgaag gggcgtagag cagccctagg accaatggaa
    27901 ggatccgttc gtctttggga cgttcacctt ccggcccccc gtggatgagt tcacgttcaa
    27961 agagggggta gccatacttt aagacgacca acgcgatggc ggcgctcatc gttttgtccg
    28021 gaacgtaacg tacccagttt tggaaccagt tcacaatcca ccagccgaca tgctcgcccc
    28081 aggtgagtcc gaagatcgat tccatccact gatgcaccat gttttcccat tccagcacgc
    28141 gggcgctgag ggtttcggcg aactccggat tgagtgcaaa tgtggcatgt tcgttcagag
    28201 cggcttggac aaaggtgccg gggatgctca tgacgaccgc gcctccgacg ccgaagatat
    28261 agagaaacca agcatgtgag aatgcggagt ttttgccggt gcgaagatat ttctgaaaac
    28321 ctgcttgacg ggccatccat ccccaataga tggcgccggt catgctgaca agcgaccagg
    28381 ggaagatcac cacatccgct cccgactcgg gaaagagcag ccaattgacg acagagttcg
    28441 agaggagtgc cgcgatagcg ccccaccagg ggcctaagag aaaggcgacc agtgccgtgc
    28501 cggtcatgtc caggaacagg atactctcca tgtggcggct cagagtcagg ccgacatagt
    28561 tcaggatgag tccggcggca acgatgacgc tcgtttcgta gagcaggaga taggatgatc
    28621 cgcgaaacgg tttcgaggaa aagaagtcga tgggtaatga ggcggcatgc gccgaacgtt
    28681 ccaccggggc aggcgtcttt cgttcttttt ccggcttgag cttgctgcgc aggtccagga
    28741 ttttctccaa gactgtgagg aaggcgccga gaaagccgac gatggctcca ccaagcatca
    28801 aaagttcttt ccccatctcg tccatcattt caggccctcc gtttcgtggt ccattctagc
    28861 caggaagggg agggacggcc agaaaaagcc tagaagccga cagggaggaa atcagcgacg
    28921 ggcgggcact gacggggatg agatgtcggc ctgagccaag ctccctgccg gaacgacttg
    28981 cgcccgttgc tgaagcgcgt gggcgatcac ggcaccgaag agaaacagcg cagccgaata
    29041 atagatccac aggaggagaa gaatgacttc gagaagcgat ccgtagagcc tggtatagat
    29101 ggtggcatat tcgttgtacg ttacaaagag cagtttggct gccacccaga ggagactgaa
    29161 cgtcaacgag ccgatcaagg cttcacgcca ttgcggccgg cggcgggggc cgtagcggta
    29221 gagaatgccg acgctcaaga aggccatggt gaagggaagc gtataggtca gaaacagatc
    29281 gtgagcagcg agcgcgatca gatcgagccc ccagaggcgc gggacgtacg cgatgagaag
    29341 attcaccatc tgggtcgcca cataggacag gacgagcaac agtccgatgg cgccttgaga
    29401 ggcgacggca atggctgtcg agatgagggg gtgccgcttc caggtgctgt caaagacgac
    29461 gttgagtgcg tactcgagtt catagaacac cagcgtgccg aaccagacaa aggatagaaa
    29521 gacgaaccag cgaaccgttt ccatttcgct gacccgctgc aactccacca ataatcgctc
    29581 tcccagggtg ggaagaaagc ccttcagaaa gctgagcagc acctgcccgc cgatgacatc
    29641 ctgactgacg acgaacccga ccgcatagag caacagaaac acgaggggaa acagcgagag
    29701 caaggagaaa aacgcgagcg aggcggccag gctggcgcaa ccatggcgca ggaaggactt
    29761 ggccaaatcc agaaggagac gccagaatga catgagtgta ggcataagcg acagcaaagc
    29821 tagcctagca tgaattgccc attcctgcac aaagggcaac gagtcgaaga agttatttga
    29881 gagagagaac ggactgaagc gcgaggctca cgaggggatt gggaaagaga ccgaagatga
    29941 tgaccccggc gaccgcacaa gccagtacga cagagagcgc gggcgaggcc accaatcgtg
    30001 gcggatccac gaccgccgcg tccggctcac gcatgtacat caccataacc acccgcatgt
    30061 agtagtaggc ggagactgcg gcgaagacca ttgcgacaac agccagccag gccagccccg
    30121 cctcgactgc cgccatgaac acgtagaact tgccgatgaa cccggccgtc gggggaatgc
    30181 cggccagcga caccataaaa accagcatca aaaacgcggc catcggctgc cgtttggcca
    30241 acccggagaa gtcttcgatt tcctctcctt ccaggccgcc tttgcggaac atcccgatga
    30301 cagcgaacgc gccgaaggtc atgaatgcat agaacgcgag atacagcatg acgctcgcca
    30361 gtcccgacgt ccctcccgat tcacccatgc tgcggcctgc cgcgacaagg ccgatcaacg
    30421 cataaccagc gtgagcgatg ctggagtaag ccaacatgcg tttgatgttg gtctggacga
    30481 tggccacgag gttcccgaga atcagggtga ccacgcagat caggagcaag agattcgacc
    30541 aatccgcttt gagccctcca agtccctcga caaagacacg caggaatgcc ccgaagctgg
    30601 cggccttcga tgcgacggcc atgaacgcgg tgaccgaggt cggcgcgcct tcatagacat
    30661 ccggcgtcca catgtggaa
//
LOCUS       DATIFR010000234        17306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1501536, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000234 DATIFR010000000
VERSION     DATIFR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501536"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..520)
                     /locus_tag="VLD60_13325"
     CDS             complement(176..520)
                     /locus_tag="VLD60_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45989.1"
                     /translation="MILGSVLGFQFNEAEAVVVVDVDVKVVEVDVEMVKVTGMDWGVL
                     VAPVAVTMMVVEYVPAASPATIAMAVNEAGAVPETGESASHGAIVLTLQFNVPVPELE
                     MATACPDGSLPP"
     gene            complement(1147..2490)
                     /locus_tag="VLD60_13330"
     CDS             complement(1147..2490)
                     /locus_tag="VLD60_13330"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HSB45990.1"
                     /translation="MRMISDVSIALMIFGLAYLVIITERIHKTIVALCGAALMIGLGV
                     VTQDEAFYSREFGVDYNVVFLLIGMMVIVNIVRETGLFEVLAIWAAQRAQAKPFRMVV
                     LLAVLTAGLSAMLDNVTTVLLMAPVTLAITKRLELNPVSFLIVEAIASNIGGTATLVG
                     DPPNIMIASKANLGYLDFLVVLGPIVIVIMAVFLVALWIFFGKSMRVAPHLQEAVLGL
                     KAGEAVQDRALLHRCVWMLGAVNLAFCFHGLLHLEPATIALLGASVFMLAGRAPKKGD
                     TEELSYLTDVEWKTIFFFIGLFILVGGLVKVGVIRYLADQLVTVTRGNLAGSTVAVLW
                     GSAVLSAIVDNIPYVAAMNPLIVDLARSLHPEIADHATLVHQPDILPLWWALALGACL
                     GGNGTLIGASANVVIVDLARRAGYQISYWQFFTLGFPVMVGSVILATVYLWLLFL"
     gene            2665..4881
                     /gene="glgB"
                     /locus_tag="VLD60_13335"
     CDS             2665..4881
                     /gene="glgB"
                     /locus_tag="VLD60_13335"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSB45991.1"
                     /translation="MSTLSRQQIDQIVKGHHWDPLAVLGLHPSSLNGSPAVVIRCFQP
                     DAIEVAVVTDKLSQSPISMSRIHEAGLFEAHLPQAPSSGRYRLRLTDGTGRVSERYDP
                     YAFAPLLTDFELHLFAEGTFFRAYDTLGSHLRSVDGIAGIHFVLWAPNAGRVSVVGDF
                     NRWDGRRHPMTSRGATGLWELFIPGLGDGTLYKYEIRPRDRDEILLKADPYAVASELR
                     PKTASVARDISQYTWNDQAWIEARRQRDPLDVPMSIYEVHLGSWMRVPEEDNRWLTYR
                     ELASKLIPYVKDMGYTHLELMPITEHPFDASWGYQATGYFSVTSRYGPPEDFMAFVDA
                     AHQAGLGVFMDWAPAHFPDDPHGLSQFDGTHLYDHADPRLGYHPDWHSRIFNYGRVEV
                     RNFLMNSAFFWLDRYHIDGLRVDAVASMLYLDYGRKAGEWIPNQFGGHENLSAVLFLK
                     DLNVLTHRDFPGTMMLAEESTSWAGVSRPTYAGGLGFTFKWNMGWMHDMLEYFSKDPI
                     HRMYHHSQLTFGLLYAFTENFVLPLSHDEVVHGKRSLIEKMPGDDWQKFANLRALYAL
                     MYGHPGKKMLFMGGEFGQRREWNHDSSLDWHLLAYQPHRGLQRLVQDLNRIYQGEPAL
                     YEIDHDWQGFQWVDFHDAAQSVLAFMRKAKDLDNQILCVCNFTPVPRYDYRIGVPLKG
                     YYRELLNSDASIYGGGNLGNAGGLAAQEVGSHGFPYSIPLTLPPLSVLFLKRQPLP"
     gene            complement(4964..5470)
                     /locus_tag="VLD60_13340"
     CDS             complement(4964..5470)
                     /locus_tag="VLD60_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB45992.1"
                     /translation="MSGLLVSGSLASAASGEKKVPTKIVDGTQVSLEYTLTLEDKTVI
                     ETNVGREPMTYLQGAHEIVPGLEKGLVGLTKGEKKRVVVKPSEGYGEVDPKAVQEVKK
                     SMIPEQARKVGAQLEAKSPDGQSMFPVVTAVNDETVTLDFNHPLAGKMLVFDVKVLEI
                     RAGEKKNP"
     gene            complement(5552..6121)
                     /locus_tag="VLD60_13345"
     CDS             complement(5552..6121)
                     /locus_tag="VLD60_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HSB45993.1"
                     /translation="MQQADWAWTRRSLLKTSVVGLLLLGGRLLAPSVARARTEELPEG
                     ELSFYNVHTNERLRVRYRGGNGQYDLSALDDVNHILRCHHTGEVATIDPRVIEHINLV
                     QKSLGGDGEIHVISGYRSPEYNAMLVKRSRRAARHSLHVEGQAVDFFIPGVRPRAIRH
                     AALKLQYGGVGYYPRADFIHLDCGPFRSW"
     gene            6530..6892
                     /locus_tag="VLD60_13350"
     CDS             6530..6892
                     /locus_tag="VLD60_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB45994.1"
                     /translation="MATILVIDDEDSIRHLLKEVLEKAHHRVLEARDGREGLTLFQNN
                     KVDLVLMDILMPGTDGLEATLQLTREYLDAKVIAMTGAQGDRNFLDVAKLFGARRVFE
                     KPFDLEKMVEAVNEELAR"
     gene            7089..7508
                     /locus_tag="VLD60_13355"
     CDS             7089..7508
                     /locus_tag="VLD60_13355"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HSB45995.1"
                     /translation="MVGILPLSNFVGHSHWEYIKWLPTADDLHSPKYLVDILTDLVGN
                     TALFLPLGIFLSRVLSSFTTSRQLVLAAGISGILSLNIEFYQVYCHNRFPSFFDIITN
                     VTGALIGVYLCVSRMGVLPATGSPTLTPIPPDRTPAP"
     gene            complement(7471..7884)
                     /locus_tag="VLD60_13360"
     CDS             complement(7471..7884)
                     /locus_tag="VLD60_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HSB45996.1"
                     /translation="MIRFTAVAILSVLVTACAPKPILYPNAHYKDVGAETADHDIEEC
                     SAMAKDAGATPSQGKTGQVAGNTTAGGAVGSAAGAVGGAVVGHPGRGAMVGAASGATA
                     GFLRGLFRRSPPSNAYKQFVQRCLKERGYDPVGWE"
     gene            complement(7939..8793)
                     /locus_tag="VLD60_13365"
     CDS             complement(7939..8793)
                     /locus_tag="VLD60_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45997.1"
                     /translation="MFKIKKKTEKPKPTVLYNIIEDYSEFDAPGNVTACAMTLTEDLP
                     GHAKILSESYDVPLEIMTTLLMDGGVYVYPQVGGLLTRGLFACLVDQTGDSPKQTFIL
                     DLMQFAEAEQLVRARSISLAEAAMEVFRRTLPDELKTKLQQKNLGLDYRMLDRAVAKG
                     GDIKYIDFRKDWSPHFKRLCIMPDGRLVETGGLEEFSQLHGITTAQAKTLVEQGGTLE
                     VGGEVLACQIVNGQPAVARFSAKNYTKAKELVESKGLHLMDALSEVGYNDPAMMRGLV
                     RKELTGGR"
     gene            complement(8840..10618)
                     /locus_tag="VLD60_13370"
     CDS             complement(8840..10618)
                     /locus_tag="VLD60_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSB45998.1"
                     /translation="MGREEENTPFLVESVSTDPIDRVIRYHVQTKHHFNRYARSLGFL
                     DWANQPDPFRRYEGASLIPLPLLKPDDEPVSPAYDAIYQAGSVHPQPITVRTLSRFFE
                     FALALSAWKKAGESEWALRSNPSSGNLHPTEGYLILPRVDGLDLKPGLYHYAPKEHGL
                     EFRAEFPAEQVARLLVPFPPGSFLVGLTSVHWREAWKYGERAFRYCNHDVGHAIGTAR
                     IAAATLGWNMALLDSVEQNTVALLLGTHRTDDFADVEPEHPDCLAVIWPSGNVRSQAS
                     GVIGGRKEVPLFLDPGLVKDLAGGRRHGKANRLSREHGVHWDIIDEAADASWKTQADE
                     RSVPLCSPPISIAETPPHPSPLTPHGSPMAGTIIHQRRSAVAFDGRTGVSAAIFFRMM
                     QRVMPRIDRLQLDRPVPWDLWPYNPAIHLMLFVHRVDGLTPGLYFLVRDPKKLAFIQQ
                     SMNPELSWTKAPGCPDDLPLYWLLEGDAKKLAVQVSCHQEIAGDSAFSFGMLAEFEGL
                     LRERGAWWYPRLFWESGLLGQVLYLEAEAAGVRATGIGCFFDDPVHEIVAVKGLSLQS
                     LYHFTIGGPVEDRRLMTLPPYIHLNR"
     gene            10746..11168
                     /locus_tag="VLD60_13375"
     CDS             10746..11168
                     /locus_tag="VLD60_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB45999.1"
                     /translation="MQFDAALAAQDTFRRAEAELGAEWETAVELEEIFSSNAGLPARE
                     AYEGLLSLASRHPNAHSFHAFCIYITWQQVTEETIARHFHTGMRLSEAYLATPDGKNQ
                     RDIAYVTELYESFRAGLGLEEEDEIQVEFRRDTPKGGD"
     gene            11185..11949
                     /locus_tag="VLD60_13380"
     CDS             11185..11949
                     /locus_tag="VLD60_13380"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB46000.1"
                     /translation="MSEDDKHRARIFLHRGDLVQARAAWETAVTDDRAASNQRELSDS
                     LGNLGNTCALMHDFEAAEQCYREVLTIQRTERNQHAIAHTLVNLGNLHIGADHPENAR
                     PYYLEALDLLRSLHDDRALGILYNNLALQEAREQKWEQAIASFTQALDYHRVVGNEEG
                     LAVTYSQMGKCFLDQGDLVKAERCLNNASEHYIKLGNEPAEAAVLRLLGSLYDKRHDR
                     ISARRCLERVVLIDQRYHLTETQSDVSSLSKLIPQE"
     gene            12145..12462
                     /locus_tag="VLD60_13385"
     CDS             12145..12462
                     /locus_tag="VLD60_13385"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB46001.1"
                     /translation="MNIDAFRQMVTKNPKGFLGRYGLGNKILQEGGSLEEAAEHLRVA
                     VQLDPLHVASHLALGRALVGLKKAEEAKPVLQAGIDAAVSGRSNGGRDLVPEMQALLR
                     TLG"
     gene            12501..12701
                     /locus_tag="VLD60_13390"
     CDS             12501..12701
                     /locus_tag="VLD60_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46002.1"
                     /translation="MNLDDARKRIDSAVTQYGQHAAPAIDLVMSEVRSDLGAKAFNEL
                     VEEFDLELLYNIAPLESDYSNS"
     gene            12718..13797
                     /locus_tag="VLD60_13395"
     CDS             12718..13797
                     /locus_tag="VLD60_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46003.1"
                     /translation="MPVIWAAISGHGYGHAAQVVPVLNKLGTLVPNLKAVLRTTVPGS
                     FFQDRLTIPWDVQAVRQDVGCVQKGPLQIDIPATWEGHTRFHAQWEQRVEQEVSALKS
                     AGPQVILADTPYLAVCAGDAAGTPTIALANFTWNEVLESFADPHQPHHQAILAAIRQS
                     YGHADLALRITPGLALASCSKVLDIGPIAEPAPSRRQELREHLHLDPTEQVVLIGFGG
                     IPLTSLPWDQMKQMLGYHFIVDGISVQSSPRIHSLSTLPFSFKTLLASVDIVMTKPGY
                     GTIVEAVALGLGVIYVRRYNFADEAPLVSFLHQYGRGHELSLADFSSGHWQPALEAIR
                     HAPTALTPPPCTGADDAARQLVRYF"
     gene            13951..14553
                     /locus_tag="VLD60_13400"
     CDS             13951..14553
                     /locus_tag="VLD60_13400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB46004.1"
                     /translation="MDTPTRRATSSIDPVVLANVAKGDSQAFSQLYDHSSTLLYTLAF
                     RILGNREEADELLQDVYLEVWRKVARYDVGRGTPIAWLITLTRSRAIDRLRTRIARGH
                     NATFPLDAAVSSNVADLGPSPFETQADQELRMAVGSAITSLPPAQQQAIELAYYEGLS
                     HQEIAARLNQPLGTVKTRIKLGMSKLRETLRHYWDQGDPG"
     gene            14550..15560
                     /locus_tag="VLD60_13405"
     CDS             14550..15560
                     /locus_tag="VLD60_13405"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSB46005.1"
                     /translation="MTHDELEESVPLYAAGTLDRGERQALEAHLLSGCASCHNALKEY
                     QSAASLLPFGLSPMQAPRALKAKIMAARNPEPVAVELGAKESIRPSLEPGEWLNHLFP
                     PVAPARSFSLPWVIGFATLLVVGFGGYLAWTYSTQLTEDAAKVQQLVTSLQEQSTRLA
                     GLQRELSTRDRMLADLQSEIQRKTNDTAELKNQLMEREVELEAAHAQLSAGARGRTPH
                     DELAALLRRPDAKVVTLAGSDMAKRATAILLYDVRTQKAWLYSANLPKCPSGMTYQLW
                     AIDQSPVSIGTFHMEAGETAHLLVKRLPDFEKAKRFAVSLEPAGGRSQPTGAIYLLGQ
                     TS"
     gene            15651..16121
                     /gene="tadA"
                     /locus_tag="VLD60_13410"
     CDS             15651..16121
                     /gene="tadA"
                     /locus_tag="VLD60_13410"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HSB46006.1"
                     /translation="MPVGEQPQDSAFMRVALDVAARATAIGEVPIAALIVQNETILAQ
                     SHNYRETWQDPTAHAEMIVIREAAKALHSWRLNESTLYVTVEPCAMCLGAIILARIPR
                     VVFGAYDPKAGACGSVLDFTNEPRLNHGVEVVGGLLEQESQTLLQDFFRQLRAG"
     gene            complement(16143..17021)
                     /locus_tag="VLD60_13415"
     CDS             complement(16143..17021)
                     /locus_tag="VLD60_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HSB46007.1"
                     /translation="MGTGIFQLVFFRAKSRYDDEMAADVKFASGLFVSSGTGEPVVTF
                     PSIDLIATAGRERSIACDDRTIHVWGFSLDGSDAAVERCRAWLSGDERARAARFVRQE
                     DQRRYLLAHGGLRAVLSCYVGLEPGILTFHVGPTGKPALADGSRSHNPLRFNLSHSHD
                     RMLVAVAKDQEVGADLEQIRDKVEAAKLAERFYAPSERNRVASVTGLKQVHQFYRYWV
                     AKEAVLKGQGVGLLSLPQCEILASDTASRAEVHLLTGETMQPGWTTHWLDCGPCWAGA
                     VSAYGSNWIVRTMTRE"
BASE COUNT         3932 a         5307 c         4432 g         3635 t
ORIGIN      
        1 cgatcgtcgc cgggctcgcc gcaggaacgt attcgaccac catcatcgtc accgcaactg
       61 gcgcaaccaa cacaccccaa tccattccgg tgaccttgac catttcgaca tcgacctcta
      121 ccactttgac atcgacgatc agcctgagcc cgattggcct cgccttttca gcacctcaag
      181 gcggaagcga tccgtcagga caggcggtgg ccatctccaa ctcaggaacc ggaacattga
      241 actggagcgt gagcacgatg gcaccatggc ttgcgctctc ccccgtttcg gggactgccc
      301 cagcttcgtt caccgccatg gcgatcgtcg ccgggctcgc cgcaggaacg tattcgacca
      361 ccatcatcgt caccgcaact ggcgcaacca acacacccca atccattccg gtgaccttga
      421 ccatttcgac atcgacctct accactttga catcgacgtc caccactacg acagcctctg
      481 cctcattaaa ttggaatccc aacactgacc ccagtatcac gggatactac gtgcattacg
      541 ggacgcaatc accgaattcg gctggatctt gcgcatacgc ccagagtacc tattactctc
      601 tcgctgacct ggccagcgca tcatcgcctg cggttacgat aaccggcctt acctcgggca
      661 cgagctacta ttttgccgtg agtgcgtata acggcgttca aagcgcgtgc tcgaacgaag
      721 tgtcgaagac gacttaagca acctggataa ggagtctttg acgccgcttt cctatttcat
      781 cacctaggcg gcgggttccc ttccaggccc cctcctgtcg aaacaaagcc cccctctgcc
      841 acgtgttatt agtgtgttag cagaaaggac aactcaaagc ggaaggtgat gaaaagagac
      901 aaagagaagc aaatcaccga gaggcacaac cgtcaataaa aacggggatt gccgctccta
      961 ttccattatt aaggcgctca gcaatccccc tttcactgat tccgaagccg ctggccttgg
     1021 gcttcatagt accaaaggcg ggatttgatt tgcggccaag tcagcacctt ctgttcccct
     1081 tcacaagggt gatgaacttg acggatggaa gggagccgtc ttgcccgttg gggaagacgg
     1141 cccttcctag aggaagagca accagagata cacggtagca aggatgacgg atcccaccat
     1201 gacggggaac cccaaggtga agaattgcca gtaggaaatc tgatagccgg ctcgcctggc
     1261 cagatcgaca atcaccacat tggcgctggc gccgatgagc gtcccgtttc cccccagaca
     1321 agcccctaga gccaaggccc accacagcgg gagaatatcc ggctgatgga ccaacgtcgc
     1381 atgatcggcg atctccgggt gcagcgaccg ggcaaggtcc acaatcaagg gattcatcgc
     1441 ggccacatag ggaatgttgt ccacgatcgc cgacagaact gccgaccccc ataatacggc
     1501 aacggtggaa ccggccaggt tacctcgcgt cacagtgacc aattgatctg caaggtatcg
     1561 aatcaccccc accttcacca gccctccgac cagaatgaac agcccgataa aaaagaaaat
     1621 ggttttccac tccacatccg tcaaatagga cagctcctcc gtatcgcctt ttttcggcgc
     1681 gcgaccggcg agcatgaata cactcgcccc aaggagggca atcgtggccg gttcgaggtg
     1741 aagcaggcca tgaaagcaga aggccaggtt aaccgcgcca agcatccaca cgcatcgatg
     1801 gagtagggcg cgatcctgta cggcttctcc cgcctttaac ccgagtacgg cttcttgcag
     1861 atgtggagcc acccgcatgg atttgccgaa gaagatccac aacgcgacga ggaacaccgc
     1921 catgatgacg atgacaatcg gacccagcac gacgagaaaa tcgagatacc ccagatttgc
     1981 cttgctggcg atcatgatgt tgggcggatc tccgaccagc gtcgcggtgc cgccgatgtt
     2041 cgaggcaata gcttcgacga tcaagaacga cactggattg agttcaagtc gttttgtaat
     2101 ggctaaggtg accggcgcca tgagaagcac cgtcgtcaca ttatcgagca tggcggacaa
     2161 acccgctgtg agaacagcca acagcacgac catgcgaaac ggctttgcct gtgcgcgctg
     2221 agccgcccag atcgccagta cttcaaagag cccggtctct ctgacgatgt tcacaatgac
     2281 catcatcccg atcaacagga acaccacgtt ataatccact ccaaactcac gagaataaaa
     2341 cgcctcgtct tgcgtgacga cacccagacc gatcatgagc gcagcaccgc aaagagccac
     2401 gatggtctta tgaatgcgtt ccgtgatgat gacgaggtac gccaggccga agatcatgag
     2461 agctattgag acatccgaaa tcatgcgcat tgtctgtgca tccgtggatc aaccatgccg
     2521 acccattatg gccagcccgt cgctctctgg caatggaact tgtccattta ttcgccctcg
     2581 ccataaaatt tttgattctt catctttcct cttccataat gagctgtctc gccgtacgat
     2641 acggatcaga ctcacggaac aagcatgtcg accttgtctc gccaacagat tgatcagatt
     2701 gtgaagggcc accactggga tccgcttgcc gtattgggcc ttcatccaag cagcctgaac
     2761 ggctcgcccg ccgtcgtcat tcgctgtttc cagccagacg caatagaggt ggcggttgtg
     2821 accgacaagc tctcgcagtc acctatttcg atgtctcgca tacatgaagc gggccttttc
     2881 gaagctcacc tcccgcaggc gccttcctct ggccgctatc gtttgcgcct caccgacggg
     2941 accggaaggg tatcggaacg atacgacccc tacgcctttg cgccccttct caccgacttc
     3001 gagttgcacc tcttcgcaga aggcaccttc tttagagcct acgatacgct aggctcccat
     3061 ctccgttctg tcgatggtat tgcaggaatc catttcgttt tgtgggctcc caacgccggc
     3121 cgcgtcagtg tcgtcggaga tttcaaccga tgggacggac gccggcatcc catgaccagc
     3181 cgcggagcga ccggcctttg ggaactgttc atccccggcc tcggcgacgg tactctgtac
     3241 aagtatgaaa tccgtccgcg ggatcgcgat gagattcttt tgaaagccga tccctatgcc
     3301 gtggcatctg aacttcgacc caaaaccgcc tccgtcgccc gcgacatttc gcaatacacg
     3361 tggaacgacc aggcctggat agaagcgaga cgacaacgag atcccctcga tgtcccgatg
     3421 tctatttatg aagtccatct gggctcctgg atgcgggtcc cggaggagga taatcgttgg
     3481 ctgacgtacc gtgaactggc gtctaaactg attccttacg tgaaggacat gggctacacc
     3541 catcttgaac tcatgcccat taccgagcat ccattcgacg cctcatgggg gtatcaggcg
     3601 acagggtatt tctccgtgac cagccgctac ggcccgccgg aggatttcat ggctttcgtg
     3661 gatgcggcgc accaggccgg cctcggtgtc ttcatggatt gggctcccgc gcattttccc
     3721 gacgacccgc acggcctctc acagttcgac ggcacccatc tgtatgacca tgccgacccg
     3781 cgactgggct accatccgga ttggcacagc cgaattttca actacggccg cgtggaagtc
     3841 cgcaacttcc tcatgaacag cgccttcttc tggctggatc gctaccatat cgacggcctg
     3901 cgcgtggatg cagtagcgtc gatgctctat ctcgactatg gccgtaaagc aggcgaatgg
     3961 atccccaacc aatttggagg gcatgaaaat ctcagcgccg tgctgttctt gaaagatttg
     4021 aacgtcctca cccatcggga ttttcccggc acgatgatgc tggcggaaga atccacctcc
     4081 tgggccggcg tttcacgtcc gacctatgcc ggaggactcg gctttacctt caaatggaac
     4141 atgggctgga tgcacgacat gctggaatat ttcagcaagg atcccatcca tcggatgtat
     4201 caccacagcc agctcacctt tgggctgctc tacgccttta ccgaaaattt cgtccttccg
     4261 ctctcacacg atgaagtcgt ccacggcaag cgatcgttga tcgagaaaat gcccggagat
     4321 gactggcaga agtttgccaa cttgcgcgcc ctctatgcgc tcatgtacgg ccatccgggg
     4381 aaaaagatgc tgttcatggg cggggaattc ggccagcgac gggaatggaa tcacgacagc
     4441 agtttagact ggcatctcct cgcataccag ccacacagag gacttcagcg tctggtccag
     4501 gatctgaatc ggatctacca aggggagcct gcgctctacg agatcgacca cgactggcaa
     4561 ggattccaat gggtcgattt tcacgatgcc gcccaatctg tcctggcctt catgcgaaaa
     4621 gccaaggatc tagacaatca gatcttgtgc gtgtgcaact tcaccccggt gcctcgctac
     4681 gactatagga ttggcgttcc tctcaaggga tattaccgtg aactcctcaa ttcagacgcg
     4741 tcaatctacg gtggagggaa tcttgggaac gccggggggt tggccgcgca agaagtggga
     4801 agccatggct ttccctattc aatccccctc acactcccgc cgctctcggt cctcttcttg
     4861 aagcgtcaac cgctaccgta gccgacaacc acgctctcaa cacaatcgtt gccctcgacg
     4921 gacagcaaag catcatgctg gcggacagcg ccagaccgct cttctaggga tttttcttct
     4981 ctccggcccg tatctcgaga accttgacgt cgaaaactaa catcttcccg gcaaggggat
     5041 ggttgaagtc caacgtcacg gtctcgtcgt tcaccgccgt caccacggga aacatcgact
     5101 ggccatcggg acttttcgct tccagttgcg cgccgacctt tctggcctgc tcgggaatca
     5161 tgctcttctt cacttcttgc acggctttgg gatctacctc accataccct tcgctcggtt
     5221 tcaccaccac gcgcttcttc tcgcccttcg tcaaaccaac taaccccttt tccagcccag
     5281 gcacgatttc atgggctccc tgcaaatacg tcatcggctc acgaccgacg ttggtttcta
     5341 tgacggtctt atcctcgagc gttaacgtat attccaatga cacctgtgtc ccgtcaacga
     5401 tcttcgtcgg gaccttcttc tcgccgctgg cggctgatgc aagggacccg gagactaaca
     5461 atccactcat cacgacagtc cacaccaatc ggatcggaca caggaagctc ttcacgagta
     5521 ccccctacga aatggtgaaa agggacagga attaccagga gcggaaggga ccacagtcga
     5581 gatgaataaa atcggcgcgc ggatagtagc cgacaccgcc gtattgcagc ttcagtgcag
     5641 catgccgaat cgcccgtggg cggacaccag gaatgaagaa atcgaccgct tgcccttcca
     5701 catggagact gtgacgggcg gctcgtcgac tccgcttcac cagcatggca ttgtactcgg
     5761 gggaccgata gccggagatg acatggattt ctccgtcacc cccaagcgat ttctgaacca
     5821 gattgatatg ctcaatgacc cgtggatcga tcgtcgcaac ttcacctgta tggtggcagc
     5881 ggaggatgtg attcacgtcg tccagcgcgg agagatcata ttgcccgttc ccgccacgat
     5941 accgcacccg cagccgttca ttggtatgca cgttataaaa cgacaattcc ccttcgggca
     6001 actcctcagt ccgtgcgcgg gcaaccgacg gcgccaggag tcgtccgccc aggaggagca
     6061 gcccgaccac agaggttttg agtagcgaac gccgagtcca cgcccagtcg gcctgttgca
     6121 cggatactct ccagaagaag atatgagggg tctacgtacg tagttgcgaa agcgcttgta
     6181 ccaaaaggca gcatccgggt caaccatttt cttctcccct tggcacaggc cgcccactcc
     6241 gccctctctt cgatcacatc accgtgatgc ccgctcagcg caaccggcgc atgccgcaac
     6301 gagctgcttc gtcagtccat tcaaatgatc cgtaggcggc tatcgtgtca tgtgctgtat
     6361 actccactaa ggtattccct cgttatcccc tctcccgcaa agggcaactg attgaaggaa
     6421 tcgtgggaga caacatcgat gacatcaata gcgggacaaa tcattttctt cgtagacagg
     6481 tgggcccaag acgggtaaga tccaaaaagt tcaaggagca ctgagcgcta tggcaaccat
     6541 cctggtcatc gacgacgaag attctatccg gcacttgctc aaggaagtac tcgagaaagc
     6601 tcaccatcgc gtgctcgaag cccgggacgg ccgggaagga ctcacgctgt ttcagaataa
     6661 caaggtggac ttggtcttga tggatatcct gatgccgggc actgacggac tggaagcgac
     6721 gcttcaactc accagggaat atctcgatgc gaaagtcatc gccatgaccg gtgcgcaagg
     6781 ggacagaaat ttcctggatg tcgcaaagct ctttggagcg cgtcgtgtat ttgaaaagcc
     6841 cttcgacctg gaaaaaatgg tcgaggcggt caatgaagaa ttggcccgat agtgatcatt
     6901 gtctgccgca ctatccccat cagtcctcat tccccgcagc gtggctgcag aacagttaag
     6961 gcctggtaat cgaaacaagg ttccgtcatg attcggacac gcatggaagg cccaccatgc
     7021 aggaaatgaa gccccgtaga acctctgcag acttgagcta tctccttctt tggatcgttg
     7081 tgatatcgat ggtagggata ttgcccttga gcaactttgt ggggcattcg cactgggaat
     7141 atatcaagtg gttgccgact gccgacgatc tccattcgcc aaaatatctc gtcgatattc
     7201 ttacagatct cgtcggcaat actgcactgt ttctgccgct cggcattttc ttgagccggg
     7261 tgctttcctc cttcacaacg tcacgacaac ttgttctcgc cgccggtatc agcggtattc
     7321 tgtcgttgaa catcgaattc tatcaggtct attgtcacaa tcgttttccc tcattctttg
     7381 acatcatcac caatgtgacc ggagcgctca tcggagtcta tctctgtgtt tccagaatgg
     7441 gcgtgttgcc agccacagga agcccgactc tcactcccat cccaccggat cgtacccccg
     7501 ctccttaaga caacgctgca caaactgctt gtaggcattg ctgggaggtg agcgtctgaa
     7561 tagcccccgc aggaatcccg ctgtcgcacc gctggccgca ccgaccatcg cgccgcggcc
     7621 tggatgtccg accaccgctc cgcccacggc tcctgccgca gatccgactg cacctcctgc
     7681 cgtggtgttg ccggcaacct gtcctgtttt cccctggctg ggagtggcac cggcatcttt
     7741 ggccatcgcg ctgcattcct caatatcatg atcggcagtt tccgcaccaa catccttgta
     7801 atgagcgttc ggatacagga tcggtttcgg ggcgcaggcc gtgacaagca cagagagaat
     7861 ggcgaccgca gtgaatcgaa tcatgtcgaa ctcccaagga gcagaacgcc cttccgatac
     7921 cccggttgac agatttccct accggccacc agtcaattct ttgcggacaa gcccgcgcat
     7981 catcgccgga tcgttgtagc ccacctcact caaggcatcc atcaaatgca gccccttgga
     8041 ttccaccagc tctttggcct tcgtgtaatt ctttgcactg aaccgcgcca ccgccggctg
     8101 gccattcacg atctgacagg ccaacacttc accacccact tccagggtgc ctccctgttc
     8161 caccagcgtc ttggcctgag cagtggtgat cccgtggagt tgggagaatt cttccagccc
     8221 gcccgtctcg acaagccgcc catccggcat aatgcagagt cgtttgaaat gaggcgacca
     8281 atccttgcga aaatcgatgt acttgatgtc gccgcccttc gccacggccc gatccaacat
     8341 gcggtagtcc agccccaggt tcttctgttg aagtttcgtt ttcagttcat ccggcagcgt
     8401 ccggcgaaag acttccatcg cagcttctgc caatgagatc gatcgtgccc gcacaagttg
     8461 ctccgcttcg gcaaactgca tcaggtccag gatgaaggtt tgcttcgggc tatcgcccgt
     8521 ctgatccaca aggcaggcaa acaacccccg cgtgagcaac ccgcccacct gcggatagac
     8581 atacacccct ccatccatga gtaacgtagt cattatctct aacggaacgt cgtagctctc
     8641 ggagagaatt ttcgcatgac cgggaagatc ttctgtcagc gtcatcgcgc aagccgtcac
     8701 gttccctggt gcatcgaact ctgaatagtc ctcgataatg ttgtagagca cagtcggctt
     8761 gggcttctct gtctttttct tgatcttaaa catgatctat tgcgctccaa gtcattgact
     8821 tattgagtgt tatagtccat taacgattga ggtgaatgta cggtggcaac gtcatcaatc
     8881 gcctgtcttc gaccgggccg ccgatcgtaa agtggtagag cgattggaga ctcagcccct
     8941 tcaccgccac gatctcatgg acaggatcat caaagaaaca accgatgccg gttgctctca
     9001 ctcctgccgc ctccgcttcg aggtaaagga cttgtcccag aaggccggat tcccaaaaca
     9061 aacgtggata ccaccaggcc ccccgctcac gcaagagtcc ctcaaactca gccagcatgc
     9121 caaatgaaaa ggcactgtct cccgcgatct cctgatgaca gctcacctgc accgccaact
     9181 tcttcgcatc cccctccagc aaccagtaca gaggaagatc gtccggacag ccaggcgcct
     9241 ttgtccagct cagttcagga ttcatggatt gttggatgaa tgccagtttc ttcggatcgc
     9301 ggaccaggaa atagagcccg ggcgtgagcc catcgacgcg atgcacaaat agcatcaaat
     9361 gaatagccgg attatagggc cagagatccc acggcactgg tcgatcaagc tgcagacggt
     9421 ctatgcgcgg catgactcgc tgcatcatgc gaaaaaagat ggcggcggag actcctgttc
     9481 ttccatcgaa tgccacagcg ctgcgacgct ggtgaatgat cgtaccagcc attggagaac
     9541 cgtgaggggt caggggtgag gggtgagggg gagtttcagc aatagaaata ggaggagaac
     9601 ataggggaac agatcgctca tcagcctgtg tcttccacga cgcatccgcc gcctcatcga
     9661 tgatgtccca atgcacccca tgttcacggc tcaatcgatt ggccttccca tgccgtcgcc
     9721 caccggcaag atctttcacc aaccctggat caaggaatag tgggacttcc ttcctgccac
     9781 cgattacccc tgacgcctga ctcctcacat ttccggacgg ccagataacc gccaggcaat
     9841 ccggatgctc aggttccaca tcggcgaagt catccgttcg atgagtcccc aagagcaatg
     9901 ccaccgtgtt ttgctccaca ctatccagca acgccatgtt ccagccgagc gtcgcagcag
     9961 caatccgcgc cgtcccgatc gcatggccga cgtcatgatt gcagtaccgg aacgcccgct
    10021 ctccatactt ccaggcttcc cgccaatgca cggaagtcag accaacgaga aatgagcctg
    10081 gtggaaacgg aaccaacaat cgagcgactt gctcagcggg aaactcagcc cggaattcca
    10141 acccgtgttc cttcggcgcg tagtgataga ggccaggttt gagatccagt ccatcgaccc
    10201 gaggtaggat gagatagcct tcggtcggat ggagattgcc ggaggacggg ttgctccgca
    10261 gcgcccattc cgactcccct gctttcttcc aggccgagag cgcgagcgca aactcaaaga
    10321 acctcgacag ggtccgaacg gtgatcggct gaggatgaac agacccggct tggtagatgg
    10381 cgtcataggc cggtgaaacc ggctcatcgt caggtttgag cagtggcagg ggaatcagcg
    10441 acgcgccttc atagcggcga aatggatcgg gctggttcgc ccaatcgaga aatccaagcg
    10501 agcgcgcata tcgattgaag tgatgtttgg tctgaacgtg atagcgaatc acacgatcaa
    10561 tgggatcggt cgaaacagat tccactaaga atggggtgtt ttcttcttct ctgcccattg
    10621 atgtcttgaa ctcagtctac aaagccaact cagtgaaagt aaatccgcgc gccaaccttt
    10681 cccgttgagg tattgacctc cgtttcatcc cccacgtatc ctcctgagga caactttggc
    10741 cacccatgca attcgacgct gcccttgccg cacaggacac attccgacga gccgaagctg
    10801 aactcggtgc cgagtgggaa accgctgttg agctcgaaga gattttttcc tcaaacgccg
    10861 gactgccggc gcgtgaggcc tatgaaggct tactctccct ggcatcacgc catcccaacg
    10921 cccactcctt tcacgcgttc tgcatctaca tcacctggca gcaggtaacg gaagaaacga
    10981 ttgcgcgcca ttttcacacc gggatgaggc tgtccgaggc ctatctcgct actccggacg
    11041 gcaaaaacca gcgggacatt gcctatgtga cggagttgta cgagtccttc agagccgggt
    11101 tggggctgga ggaggaagac gagattcaag tggagtttcg aagagacacg cccaagggcg
    11161 gagactgacc gcgtatcttt tgaaatgtca gaggacgata aacatcgcgc tcggatcttt
    11221 ctccatcgcg gcgatcttgt tcaggcccgc gcagcctggg aaacggcagt cacggacgat
    11281 cgggcagcca gcaatcagcg tgaattatcc gattcgctcg gtaatctcgg caacacttgc
    11341 gcgctcatgc atgatttcga ggcggccgag caatgctacc gcgaggtgct gacgatccaa
    11401 cgcacagagc gcaaccaaca tgccatcgcc cataccctcg tcaaccttgg caatctccac
    11461 atcggcgcgg atcatcccga gaacgcccgc ccctattacc ttgaagcgct agaccttctc
    11521 cgttcactgc acgatgatcg ggcgctcggc attctctaca acaacctcgc gttgcaggaa
    11581 gcccgcgaac aaaagtggga gcaggccatc gcgtcattca ctcaagcgct cgactatcac
    11641 agggtggtcg gaaacgaaga gggcctcgct gtgacctaca gtcagatggg caaatgcttt
    11701 ctcgaccagg gcgacctcgt caaagccgag cgctgtctga ataacgcctc agaacattac
    11761 atcaagctcg gcaacgaacc ggccgaggct gcagtgctcc ggctgctggg atccctttat
    11821 gacaaacgcc atgaccgaat ctccgcgcgt cgttgcctcg aacgcgtggt cttgatcgac
    11881 cagcggtatc atctcacgga aacacaaagc gacgtctcat ccttatcgaa attgatcccg
    11941 caagaataac catagttact gccttccatc ctcgtttcac atttcctgcc cctttctgtc
    12001 ggctacggcc tcccccatcg tcattccccg tccctgccct actggcacag taatcgttta
    12061 catgcaacgc cgcaaaccga tttttcgcct ggctgaatga aaacctccaa tggtatgatt
    12121 ccccatcgct tgggagcagc cacgatgaat atcgacgcgt ttcgtcagat ggtgaccaag
    12181 aatccgaaag ggtttcttgg gcgctacgga ctcggtaaca agatcttgca ggaaggcggc
    12241 agtttggaag aagctgccga gcacttgcgc gtagcggttc aactcgatcc gctccatgtg
    12301 gcatcccatc tggcgctcgg tcgagcacta gtcgggctca agaaggcaga ggaggccaag
    12361 cccgttctgc aagccggtat tgatgcagcc gtctcaggac gctccaacgg aggacgcgat
    12421 ctggttcctg agatgcaggc tttgctgcgg accttgggat aacaatcgca ccctttggac
    12481 ccttgaacag gagccccgtt atgaatctcg atgacgccag aaaacggatc gattcagctg
    12541 tgacgcaata cggacagcac gccgcccccg caattgattt agtcatgagc gaagtccggt
    12601 cggatctggg cgccaaggcg ttcaacgagc tggtcgaaga gttcgacctt gaactcttgt
    12661 acaacatcgc cccgttggaa tcggattact cgaacagcta gcaggatacc gctcaccttg
    12721 ccagtcatct gggctgccat ctccggacac ggttacggcc acgccgccca agtcgttccg
    12781 gtcctgaaca agctcggcac gctggtgccg aacctcaaag ccgttctccg caccaccgta
    12841 ccgggctcgt ttttccagga tcgactcacg atcccttggg acgtccaagc cgttcggcag
    12901 gatgtcggtt gcgtccagaa gggtccctta cagatcgaca tccctgccac atgggaaggg
    12961 cacacaagat ttcatgctca gtgggagcaa cgagtggaac aggaagtctc cgcgctgaag
    13021 tcggccggtc ctcaggtgat tcttgccgat acgccctatc ttgccgtatg cgccggtgac
    13081 gcagccggga ctccgaccat cgcgcttgcc aatttcacct ggaacgaagt cctagagtcc
    13141 tttgctgatc ctcaccaacc ccatcaccag gcgatccttg cagcgattcg gcaatcgtac
    13201 ggccacgccg atctcgcatt acgcatcacg ccaggacttg ccttggcgtc atgttcgaag
    13261 gtgctcgaca tcgggccgat cgcagaaccg gctccgtccc gccgacagga actgcgcgag
    13321 catcttcatc tcgaccccac cgagcaagtg gttcttatcg gttttggcgg catccctctc
    13381 acatccttgc cctgggatca gatgaaacag atgctgggtt atcacttcat tgtcgatgga
    13441 atatccgttc aatcttcccc tcgcatccac tctctctcca cccttccgtt ttccttcaaa
    13501 acgctccttg cttcggttga tatcgtcatg accaaacctg gctatggcac catcgttgaa
    13561 gctgtcgcgc tcggcttggg agtaatctat gtgcgccggt ataactttgc cgacgaagcg
    13621 cccctcgttt cattcctcca tcaatacggc cgcggacatg aactgtccct cgctgatttc
    13681 agttcgggac attggcagcc agccttggag gcaatccgtc atgcacccac ggctctgacg
    13741 ccgcccccct gtacaggagc ggacgatgcc gcccgccagc tggtccgata cttctagtgt
    13801 tcagaacgtc atcagtataa gccagcagca attttcatca atattcttca tgacttaaag
    13861 agagatcttt ttggcgaatt tgcccgtaga tgctatagtt gtggcgggcg taaggtgcct
    13921 tcacctcagg tctttgcgct ctcacaaggt atggatacac cgactcgccg cgccacatca
    13981 tcgatcgatc cggtcgttct ggcaaatgtc gcgaagggcg actcacaggc attcagccag
    14041 ctgtacgatc attcgagcac attgctctac acgcttgcct tccggatttt gggtaaccga
    14101 gaggaagccg acgaattgtt gcaggacgtc tacttggaag tctggcgcaa agtggcgcgc
    14161 tacgatgtcg gccgtgggac gccgatcgcc tggctgatta cactgacccg cagccgagcc
    14221 attgaccggt tgcgtacgcg catcgcccgc ggccacaacg ccacgttccc cctggatgcg
    14281 gccgtctcgt ccaatgtcgc cgacttggga cccagcccgt tcgaaacgca ggccgaccaa
    14341 gagttacgca tggccgtcgg ctccgctatc acgagtctgc cgcctgcaca acaacaggcc
    14401 atcgaattgg cgtattatga aggactgtcc catcaggaaa tcgcggcgcg attgaatcaa
    14461 ccgctcggca ccgtgaagac cagaatcaaa ctcgggatgt cgaagctgag ggagacactc
    14521 agacattact gggaccaggg tgatccagga tgacccacga cgaacttgaa gaatccgttc
    14581 ctctctatgc agccggcacc ttggaccggg gtgaacgcca agcattagaa gcccatctcc
    14641 tatccgggtg cgcctcctgc cacaatgcat taaaggagta ccaatccgcg gcttccttgc
    14701 tccccttcgg cctctccccg atgcaggcac cacgcgcgct taaagcgaaa atcatggccg
    14761 cacgaaatcc agaaccggta gcggtcgagc tcggcgcaaa agaatcgatc cgccccagtc
    14821 tcgaaccagg agaatggctt aatcacctgt ttccaccggt tgctccggca cgttcattct
    14881 cgctcccatg ggtaatcggt ttcgccacgc tgttagtcgt gggtttcggc ggctacctcg
    14941 cctggaccta tagcacgcaa ctaaccgaag acgccgcgaa ggttcagcag ttggtaacgt
    15001 ctctgcaaga gcagtcgacc agactcgccg ggctccaacg tgagttgagc acccgtgacc
    15061 gcatgctggc cgacctgcaa tccgagattc agagaaagac caacgacaca gcagaattga
    15121 agaaccaact catggaacgc gaggtggaac tggaggccgc ccacgcacag ctcagcgccg
    15181 gagcacgcgg caggaccccc catgatgaac tcgccgccct gttgcgcaga ccggacgcca
    15241 aggttgtaac cctggcagga tcggacatgg cgaaacgcgc aaccgccatt ctgctctacg
    15301 atgtccgaac gcaaaaagca tggctctact ccgcgaatct cccgaaatgc cccagcggca
    15361 tgacctatca actctgggct atcgatcaga gccctgtcag catcggcacg tttcacatgg
    15421 aggctggtga aaccgcacat ctgctggtca agcggcttcc tgactttgaa aaagccaaga
    15481 ggttcgccgt gagtctcgaa ccggccggcg gacgctccca accaaccggc gccatctacc
    15541 tcctcggaca aacatcgtaa aatcaaaacc tcccttgacc tacgcgagga aagcccgtta
    15601 caaccggccg ttgtgattga tacaagtcgt ccagtcaaaa ggaatgccta atgcctgttg
    15661 gggaacagcc ccaggatagt gcgttcatgc gggtagccct tgacgtggct gcaagagcca
    15721 cggcgatcgg tgaagtcccg atcgccgccc tcatcgttca gaacgagacc atcctggccc
    15781 aatcacacaa ctatcgagag acctggcagg atcctactgc gcatgcggag atgatcgtca
    15841 tccgtgaggc ggccaaagcc cttcacagct ggcgcctcaa cgagtccacc ctctacgtga
    15901 cggtcgaacc ctgcgccatg tgtttggggg ccatcatcct ggccaggatt ccgcgcgtcg
    15961 tctttggcgc ttacgatcca aaggccggag cctgcggatc ggtcctcgac ttcaccaatg
    16021 agccgcgatt gaatcacggc gtagaagttg tgggcgggtt gcttgaacag gagagccaga
    16081 cgcttcttca ggattttttt cgacagttac gagccggatg aacaagaaga aggatcgcgc
    16141 aactattctc tcgtcatagt acgaacaatc cagttgctgc cgtaagccga gacggccccg
    16201 gcccagcacg gcccacaatc aagccagtga gtggtccatc caggctgcat cgtttcgcct
    16261 gtcaacagat gaacctcggc tctcgaggca gtgtctgagg caagaatttc gcactgcggc
    16321 aaagacagaa gtccgacacc ctgccctttc aacactgcct ctttggcgac ccaataccgg
    16381 tagaactgat ggacctgttt caatcctgtc acgcttgcca cacgattgcg ctccgatgga
    16441 gcatagaatc gttcggccag tttggccgct tcaaccttgt cgcggatctg ttcgagatcc
    16501 gcaccgactt cttgatcctt ggccacagcc acgagcatac ggtcatgcga atgagaaaga
    16561 ttgaagcgca atggattgtg agagcgactt ccatccgcga gcgccggttt accggtggga
    16621 ccgacgtgaa aagttaagat tccgggctcg agacccacat aacatgagag cacggccctg
    16681 aggccgccat gtgcgaggag atagcgccgt tgatcctctt gacggacaaa ccgtgctgct
    16741 ctggcccgtt cgtccccgct caaccaagcc cggcaccgct ccacagcagc gtcgctgcca
    16801 tcgagcgaaa atccccacac atgaatcgtg cgatcatcac acgcgattga cctctcccga
    16861 ccggccgttg caatcagatc aatagatgga aacgtcacca ctggttcacc cgtaccagaa
    16921 ctcacaaata acccacttgc aaatttaaca tcggcggcca tttcgtcatc gtaccggctc
    16981 ttcgcgcgaa agaaaaccaa ttgaaaaatc cccgtgccca cggtacgctt ccggcagtcg
    17041 actcgctgaa gccactcatt gaccccatcc tgtcggaact tcaggcacaa cgtacgaacg
    17101 gagaggtgcg agagcggccg aatcgggcag tctcgaaaac tgtttcgacc gttcgcgtga
    17161 atcctcaaac tacgaacact gactgcgact tcgcctctcc ccgaaaaggt ctcgataggt
    17221 ccactgacat gcgttccctt tggcgtcaaa ctggacggtg agtgttccca cttcattcgg
    17281 caatcgaaca ggccattcac aggcct
//
LOCUS       DATIFR010000235        27945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2655259, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000235 DATIFR010000000
VERSION     DATIFR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27945
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655259"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..768)
                     /locus_tag="VLD60_13420"
     CDS             complement(13..768)
                     /locus_tag="VLD60_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HSB46008.1"
                     /translation="MQLTILGSGTNVHPSRAAAGYLVRTDQTLLLDFGPRTLSNLIKT
                     GVNRHRITHILFSHFHADHFSDFITFFFDAVIYAKYGGGHRPDLTVIGPKGSKRLMKT
                     IMKTFPSFSRPPFRVIFNEVGDRCFNLGETRISPRTVTHVPDLHCIGYRIDYRGKALA
                     YSGDSQYCDNLVHLCRNVDVAVLDCSFPANKPGPAHLHAGECGQVAMEAGIGRLVLSH
                     FYPIAERYNVKEQAGESFTGPITVGRDRLTMKI"
     gene            complement(782..1270)
                     /locus_tag="VLD60_13425"
     CDS             complement(782..1270)
                     /locus_tag="VLD60_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSB46009.1"
                     /translation="MVLARTILPLQMDLPDFKDDPYLKVVRPLAEAYLAFWRVDSRHI
                     RSMRLTPSQFDVIVTLGDTNGLTCSELSAQTLVTKGTLTGVLDRLAKKGLIRRESVKA
                     DKRRTKICLTEKGNALFRKVYAGHIAFLRPFFERALSPNDAEIARTLLLRLRDSFHAQ
                     NR"
     gene            1543..2175
                     /locus_tag="VLD60_13430"
     CDS             1543..2175
                     /locus_tag="VLD60_13430"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB46010.1"
                     /translation="MLRRSSGNANLTAIALLLGVVIAAVWVWKRLSLETQEYIIDQAI
                     PLVFVGALCCAVLWTVVRAVRKRKRRLSQRAKLLAAFERETARDRKLETAFALIEVNE
                     YRVQGLESVVPAMRELFVTTLQRAVGEKQHRIRGMAASHLGVLEDMTVVPLLVKALED
                     DHAYVRSCAALGLGRLRAVETRERLKVLMEEDWDQTVRSRAKEALERMSA"
     gene            complement(2179..3036)
                     /locus_tag="VLD60_13435"
     CDS             complement(2179..3036)
                     /locus_tag="VLD60_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB46011.1"
                     /translation="MTRRRMSWPDRLRSFIGYCLSEPLYRLFSLVPHWEFGLSSHRVS
                     NLTFTHGALAGRRAVHLSDLHLDRYHPRHERVVEAVDELNPDWIFITGDLLNVPEGLP
                     HLFRFLARLREVAPVYITLGNHDHYSGVSVEQYAELADRHGITLLVNQTTFIQTGTGE
                     LALAGVDDPSLHRADLSCLPPPADRRFTLLLAHAPNILDQMEEEHVVDLILCGHSHGG
                     QWRIPGIPTFWLPPGCNGRIEGEHRSGECTLYINRGMGWSFLPFRWNCEPEIAVIDWT
                     NEQTQARAA"
     gene            complement(3033..3659)
                     /locus_tag="VLD60_13440"
     CDS             complement(3033..3659)
                     /locus_tag="VLD60_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46012.1"
                     /translation="MHPGLNIVVGTVFILLLAIAPEEARSAGRIDNAVFSSFTGHITR
                     QMGLDKQAFAQASTCLSWFYKASKKKPPPPAAQGINWSPTATPPLEEECPRIYPGGMD
                     AAREDFAKTQTLLSVCLTIYEFALVADQDDNQSYSTIELQDLFQSLSLAYDTADSPRA
                     SIDTLTDRFDNWYRARTLDQVMQGMSTLYERGYRVTPNDRAELDRVMK"
     gene            3734..4789
                     /locus_tag="VLD60_13445"
     CDS             3734..4789
                     /locus_tag="VLD60_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HSB46013.1"
                     /translation="MDRIPTTQPYLTSAMPGIGGRMRVEPEDFRVDERPLYLPCGEGE
                     HLYIKVTKRLLSTPDLIRKISSTVGVKAQGIGTAGLKDARAVTTQMVSLHGVTPDRLA
                     RLKADGQILSIELLGRHRNRLRPGHHAGNQFRLVIRDVAAHAKETVPSVLDVLARRGV
                     PNYFGPQRQGRTGENYQTGVALLTDSDRRNKMSRAKRMWYLNAFQSHLFNQILARRID
                     RIDRILAGDWAMKMENGACFLVDNAETEQPRADRFEISPTGILFGSRVSWASGEPGEI
                     ERAVATGAGATPERLTEAAKACGFRGERRSLRVPLADLDWSLDGSILTLSFSLPPGAY
                     ATSVLRELMKAPEPSVR"
     gene            4903..6144
                     /locus_tag="VLD60_13450"
     CDS             4903..6144
                     /locus_tag="VLD60_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HSB46014.1"
                     /translation="MVYQETVLLQGHIIDSLILAKVLDRILSMGGSFDLQEVQIGKTR
                     EDPSRAKIVVQTESPAQLKSILEAIQPHGASVERESDCQFQPAPGDGVFPDEFYATTH
                     LPTQVRLRGSWINVDLIEMDLGIVVEPERGVARAVPMASVKGGESVVIGRQGVRVIPL
                     QRPRKRDIFSFMESQVSVERPHGHMIHDIATRMRQLRSGYREGQADSRILFSGGPAIV
                     QAGGQDALAWLIENEYVQVLFCGNALAAHDLEMELYGTSLGYAHQAGQPLPHGHEHHL
                     RTINRIRSIGSIKKAVDAGLVKQGIMAACVRQAVQVVMAGTIRDDGPLPGVITDSIQA
                     QEAMRKAIPGTGLALLVASTLHAVATGNLLPASVPTVCVDINPSVPTKLADRGSFQAV
                     GLVMDAASFLREIARELGWKP"
     gene            6141..6941
                     /locus_tag="VLD60_13455"
     CDS             6141..6941
                     /locus_tag="VLD60_13455"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HSB46015.1"
                     /translation="MSRLLVCPPDFFGIEYEINPWMHRSNAVNRERATRQWRRLMQVL
                     EQDVGVGLERMRPVQGLPDLVFTANAGVVVGRQAVPSRFRYPERQREEAQFEDWFRLH
                     GYEILTLERDHHFEGAGDLLGFQDCWFGGYRQRTDIRAFPLLSEWFHREIIPLELVDN
                     RFYHLDTCFCPLSGGELLYFPPAFDRYGQEAIADRIDPMQRLAVPEREALAFACNAVC
                     IGKHVVLPADCPDTEAWLVSRGYRPHAVPLDEFLKSGGSAKCLTLALD"
     gene            complement(6962..7312)
                     /locus_tag="VLD60_13460"
     CDS             complement(6962..7312)
                     /locus_tag="VLD60_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46016.1"
                     /translation="MKICPSCQRELPEIYRYCTECGSSLDGRPQPTSAKPDATPEALN
                     LRILYGMVAMLVLALLFPPWETPPSQAPEFLGLHFILSPPTPEAVVSRLLISIELVTI
                     AIAGLYGSFLFRSR"
     gene            complement(7383..8087)
                     /locus_tag="VLD60_13465"
     CDS             complement(7383..8087)
                     /locus_tag="VLD60_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB46017.1"
                     /translation="MNIKRTINRVRGAIFLTIMFHAILLPSMTEAESYVAGTFGVTMP
                     QSLSNGVVTQDGFGGLDISDQSLKSSVNVGAKLGHYFSKARWVGIETGLSYTTPHIKE
                     GSLTFTGPGGSLTSGTLSGVHQRIIIWDVVTFMLRYPGNRFQPYVGVGPALFFASLNG
                     PTAPPGQSATAIGLNAEAGARYFITRHWALFGEGVYNRARLSYSSNDSNPAADPFGFR
                     ATYSAFTVSIGVSYHF"
     gene            8407..9426
                     /locus_tag="VLD60_13470"
     CDS             8407..9426
                     /locus_tag="VLD60_13470"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSB46018.1"
                     /translation="MPTIHRIGVIGAGAWGTALAKHLAEKGLHTTLWAYERDVVDSIT
                     AKRENQLFLPGVTLPSALVVTNTLVEAIEGSDGLLFVVPSHVARQVLRQVAPLLSSST
                     PIVSATKGVEEDTLKLMTQVMEDVLSPPLHDRLMVLSGPSFATEVSQGQPTALCLAGK
                     DGLLVEAFQTVFMTSALRIYADSDVIGVQLGGALKNVMALAAGVVDGLGLGHNTRAAL
                     ITRGLAEMVRLGNAMGADPRTFYGLSGVGDLVLTCTGALSRNHTVGVRLGKGESLETI
                     LGGMHAVAEGIRTSRAAAGLALRHRVEMPIVREINAVLFEGKSCRKAVGDLMERVAKP
                     EKGQT"
     gene            9423..10211
                     /gene="larB"
                     /locus_tag="VLD60_13475"
     CDS             9423..10211
                     /gene="larB"
                     /locus_tag="VLD60_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSB46019.1"
                     /translation="MNPARLERLLTNVRSGTLSVTKALEQLRSLPFEDLGFASLDHHR
                     SLRQGFPEVILCEGKTLSQIQRIAKALLKHHRPFLATRATRQVASLIKRIDGRAQYHE
                     DARLVAIRDPKRPLQGHVLVITAGTSDVPVAEEARISAEVMGSHVETLYDVGVAGIHR
                     LLDRHHRLTEANVIVVVAGMDGVLPSVVGGLVDVPVVAVPTSRGYGASFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINSLGVKRNERVMRDKRER"
     gene            complement(10279..10830)
                     /locus_tag="VLD60_13480"
     CDS             complement(10279..10830)
                     /locus_tag="VLD60_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46020.1"
                     /translation="MFFTALSSSSPSTDFRVRHHHWRWTVAILAIALLLPAASSAKTK
                     PNRAPRPEPELKVLELKVSPNPYTVSAGSLEFSALVQLPMELNGATLLEVSSLVSSPS
                     KTSIRFLSIRKPLEPHSTPENSADRPKISVVLAWDGMDHKKTPAEAGLYQYELRAKLL
                     TNGEKGPRTLMVSWPKRGTLEVK"
     gene            10985..12187
                     /gene="larC"
                     /locus_tag="VLD60_13485"
     CDS             10985..12187
                     /gene="larC"
                     /locus_tag="VLD60_13485"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HSB46021.1"
                     /translation="MSLHLHFDCFSGISGDMTLGALVDAGAPFPDLVKGLKRLGVAGF
                     TLRKRRVQRGGISATKVDVVIGAGLRHPLSLPRIRKILSASRLSPKIKEQSRAVFERL
                     AEAEGKAHRVAKDHVHFHEVGVLDSFVDIIGGLICCELLGVTRVTASPVNVGAGTLRS
                     AHGILPAPGPAVAILAKGLPIYSAGPSRELTTPTGLALLQTLTTEFGPMPLLQPTSVG
                     YGAGDANPAGWPNVLRVFLSRDSSRGGRGRDTVLQIETNLDDLNPQTYEHIMERLFRS
                     GALDVTLTPVIMKRGRPGIVLTCLVVPTGLHPLLNVLFEETTALGVRVQEVGRHILPR
                     RFTSVKIRGGEVRVKIAAVDETVTKAAPEYADCQRIAQRTGRPVKAVLEEAALAYAKG
                     RWPAKDHS"
     gene            12184..13191
                     /locus_tag="VLD60_13490"
     CDS             12184..13191
                     /locus_tag="VLD60_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46022.1"
                     /translation="MTLLLYSLLFIASVAVTLGACTLFTNAIEWLGKRLNLSEGAVGS
                     VLAAIGTTLPETSIPIVAIFFGTSRQEAEVGVGAILGAPFMLSTLVVPILALLLLLFS
                     RLGKRSPLFRLNYKDVTVDLSFFAFGYTVAMACAFIPSRLLHVIAAIGLMTCYLYYVK
                     LKFSDSGEEGDGGELDPLFFFGRKSATPSYGLIALQAIAGLAGLALGAHLFVTAAEST
                     AQFLGISPLILALLIAPLATELPEMSNSFLWLYQKKDRLAVGNVTGAMVFQGMFPVSI
                     GLLGTDWTLAPNALITMVLAVVAAGSLLIQTAWAGHWRPWLLSAGAVLYIGYALYLYG
                     S"
     gene            13219..14262
                     /gene="pdxA"
                     /locus_tag="VLD60_13495"
     CDS             13219..14262
                     /gene="pdxA"
                     /locus_tag="VLD60_13495"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSB46023.1"
                     /translation="MGTKNNRVIQKSKPLLGITMGDPAGIGPEVIAKALSGTKLRRVC
                     FPLVIGSLPVMQQTVKKLRLKLDVRSVDGHDRNALRANQIAVLDPLEKPLGSFKVGVA
                     APETGAASVAFIKKAVHLAQIGCVDGMVTAPINKEAINMAGCHFPGHTELLAELTHTE
                     ESGMMIIGGPLRIMFVTTHVAIKDLPSLLTQDKIERGIRLAHLALTTLFGIKKPRIGL
                     AALNPHAGEHGLFGDEEAHVVLPAARAAQAQGILASNPLPADSLFGKAARGDYDGVVA
                     LYHDQGLIPLKLVAFGTCVNLTVGLPIIRTSVDHGTAFDIVGKGIADPGSLIEAVSLA
                     AELATKRQSRKAI"
     gene            14280..14606
                     /locus_tag="VLD60_13500"
     CDS             14280..14606
                     /locus_tag="VLD60_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46024.1"
                     /translation="MSQMTNKEQAVVQQQLKELDQIFQDTMETLNTVAGMERVAKWKA
                     KTVALLSEAVGQQAGQDFARVQPGPSFTNDLVEEFSDFIDCFKTPLSALSKKLSQAGP
                     RPSGGG"
     gene            14610..15407
                     /gene="rsmA"
                     /locus_tag="VLD60_13505"
     CDS             14610..15407
                     /gene="rsmA"
                     /locus_tag="VLD60_13505"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSB46025.1"
                     /translation="MPSPTFPPANKRLGQNFLIDPNIVRKIIALAELTPQSAVLEIGP
                     GRGILTEALSRDAGQVVAIEIDPVLHRLLQERQADWPNVELVCGDALAYPVENLPTGT
                     VVVANLPYYISTPLLFRLLDCRSRFPRMVLMLQDEVADRLVAKPGSSDYGVLSVMAQY
                     AADITKAFKVSAQCFRPRPEVGSAVVLLRSKAQVLLRPEEKPAFAALVKASFAHRRKM
                     LVNSLRDEDYDQQLVANALNDMGIPLTIRAETLALDQFMELTKRLAV"
     gene            15482..17905
                     /locus_tag="VLD60_13510"
     CDS             15482..17905
                     /locus_tag="VLD60_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HSB46026.1"
                     /translation="MGVTSSAKRGEARRAPAPPSHIRREVIGVILIALSLLTLLSLLS
                     FVPGEPKVVATPGSTGSPPTHNLIGSVGAVFSSVLFSLIGGAAYLFPILLGLMGIRCF
                     TQSDLSIRLRNAGASLGALLFLSAFLHLEVMAVPTLSSGLVFRGQAGGLFGQILAEGL
                     RVYFASTGAHILIIAGFLVALLFTTPLSLAQIAIQVPDWGRKLLEAARALIPERTNEE
                     PVTEPSKVSKPKKAKSIRAVIEEVIPQPVETPPAVEPVIQPQLVAVPEQPDVAEFEPE
                     GMVSGKIEDPDDYQLPDPDELLSEPSGVLSRVTDEELKTQSEILSKALLSFGIEGKVT
                     EVRPGPVVTMYEFEPAPGTKVARIVNLADDLALAMKAISLRIVAPIPGKSVVGIEVPN
                     AQREMVSMKEVATSEAFTRARSKLTLALGKDIFGAPVIADLKTMPHLLVAGATGAGKS
                     VGLNTMLISILLSARPNEVKLLLIDPKMLEFQTYDGIPHLLRPVITDPKSAARGLSWV
                     VQEMERRYKLLAEAGVRNIDSYNRKVAGAQGVSGDSGTETKPDQPELPMQFLSEEERL
                     SAGETIIPEGSPGSFAPPPTPPEPLPYIVVMIDELADLMMVAPKDVEDKIARLAQMAR
                     ASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEALLGRGDM
                     LYLASGTGRLMRLHGSFVSDDDVRRVVEFIKEQALPSYNQELQSLKQEEAKEEEAQDE
                     VYEQAKDLVLSTGQASASLIQRRLRVGYPRAARMIEQMEADGLVGAAGRDGRREVIGR
                     RGPVGDAQG"
     gene            17902..18612
                     /locus_tag="VLD60_13515"
     CDS             17902..18612
                     /locus_tag="VLD60_13515"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSB46027.1"
                     /translation="MNHFWIHGAVLLALTLSSVALAEDAVDEQARKEVREVVKRLQAR
                     YEKTKDLQADFTQKTTIEGFERSMNSSGKVYIKKPGRLRWNYLDPSTEDIYVQGDDVK
                     VYVPEHKQVLVGKLTQMAASKAPLELLQGAAKLEESFDVEPTPGKGRGVGGIRLLTLL
                     PKSREGEAHRTLQKIVVEVFPKTYYIRTVSLYEISGNVSNFEFSSLQTNVGLGDDVFN
                     PKLPADVEVVKAPVLNQP"
     gene            18659..19417
                     /locus_tag="VLD60_13520"
     CDS             18659..19417
                     /locus_tag="VLD60_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSB46028.1"
                     /translation="MSTGVLSQVAEATDQAVARLDVDRLHREYWEQNEFLFIPQFLPR
                     AFVEATLVPQAQGVKGDLNRNFIPGHKKGGSVSYYTVQEKAPLFLDLYRSPAFLNFLS
                     RLVEAKLMLCPDNDPHSCALYYYTEPGDHIGFHYDTSYYKGARYTILMGLVDKSTQCK
                     LVCELFKDDPVKTPRKLELVTQPGDLVIFNGDKLWHAVTPLGQGEERIALTMEYVTNP
                     EMGPFKRLYSNLKDSFAYFGIRGVFKRAWSASHE"
     gene            complement(19485..19973)
                     /locus_tag="VLD60_13525"
     CDS             complement(19485..19973)
                     /locus_tag="VLD60_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB46029.1"
                     /translation="MQKAAAVTSSVEKKRLEQPEILIVDDDPDLQIILHDLLESEGYA
                     VTEASTCREALLRAQTQFFDAVLLDIGLPDGDGLSVLNHFQESVPSLSVIMLTAATST
                     DLYADVLAQGAFTFLSKPYDHNELRLLVRQAIRTTANNGPTDNRTIHEPTGSEARLNG
                     VR"
     gene            complement(20030..20440)
                     /locus_tag="VLD60_13530"
     CDS             complement(20030..20440)
                     /locus_tag="VLD60_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46030.1"
                     /translation="MTMISQATSTAAEEKLRRAMQLSGTPVIKAIEAKPRMVRLTVNL
                     PSDLVEQMRDAVYWTPGLTLAWMVARSVRTSLAELEQAHQGPFPKRSKPLRAGRPRLV
                     GQSMKVQAQPRNSRETSSGDRSMQTRVVSEPLKE"
     gene            complement(20975..21484)
                     /locus_tag="VLD60_13535"
     CDS             complement(20975..21484)
                     /locus_tag="VLD60_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSB46031.1"
                     /translation="MDALQSIVTDLNTLIPILNHWFHLLSAVIWIGGLAFLVMAVTPG
                     LQKAVAKEQIKPITDIFYKYYKKVAGILLVVLLFTGGINLHYVNQVLTSQTGSGVQHH
                     AKYLTVFFIKLSLVLGLLTLFLYTVIFKSDDETEEGESYEVIPFQRAALWMGFFIILC
                     AAAMKHLHQ"
     gene            complement(21589..22974)
                     /locus_tag="VLD60_13540"
     CDS             complement(21589..22974)
                     /locus_tag="VLD60_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB46032.1"
                     /translation="MGKSLPIFNGPSLSGALGSFQQQITQFFTPAPQGAGPYDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKSAGGDDELSTEECYRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARRAVKLGFMVVFGTNGMLIDDQMAKTLVEIGVMGVGISIDS
                     LDPAKHNSFRGVPGAWEAAVAGIEASKRNGLQFQVHFSAQPMNYQELPAVVEWSHGIG
                     ARVLNVFFMVCTGRGEELTDITPAQYEEVLGYLVNCQDNYKGMLVRARCAPHFKRLAY
                     EKDPNSPITKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRQNSFVDLWEK
                     SDIFNSFRYPQLKGKCGDCEYTDICGGCRARPYVDHGDWLDEDQWCLYTPKGGEKIKV
                     AFNIQEETEVSWDEASALRLSRIPYFLRAMVKKGVEKHARENGISTVTIELMEELRKK
                     RFGNDAPVFKF"
     gene            complement(23061..25148)
                     /locus_tag="VLD60_13545"
     CDS             complement(23061..25148)
                     /locus_tag="VLD60_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB46033.1"
                     /translation="MYKTIYVPVDNSDHSNTAVDVGVHLAKMFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPV
                     ERRSLEGRNWKVLAEDINTNAYDLVIMGALGVGAVKDSVIGSNTERVVRRVRNSDMLI
                     IKSTQPITSGKIVVAVDGSPYSFGGLMTGLSLGKALNMPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEIIDSGLAKIYQSHLDISREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKDVPALLIVGRIGVHSDEDMDIGSNTENLLRSAPCNVLVSNRKYVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNTVVDAAVGHI
                     LPKGAMDAMRALGGSLEAAGIDRDKMQADDSVAKDLMGSTLSGMMTEIVEEKPTVSAG
                     TQAYLDRMTQNYFVCDGCGYIGKGETPVKCPVCGAEGARFKQVDKSIFEAAAKAEGGV
                     ETDVAYDDVPMQWTKDAKEAIRAVPAGFQRRRAKAKIEKTARKLGMTTITLEYAAPMI
                     KEAASEDYQPIFANKGAGTSAEAEARLAAVNGNGTHENGNGSGNGEAEASPYTWTIDA
                     QARLDRAPEGFMRDCTKALIIKHADKIGTTLITLEVANEGIEQAKGYMEEAMKTGNLK
                     DMIANLTGTGKKG"
     gene            complement(25306..26934)
                     /locus_tag="VLD60_13550"
     CDS             complement(25306..26934)
                     /locus_tag="VLD60_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB46034.1"
                     /translation="MNSILYIFLPCKKVYPIGITYLADFIHRRKPEVRQRILDLSLFP
                     VSKRSQAIRDTATEFKPDLVCFSWRDIQIFSPHEGDSSLEHAFNFYFASNPLKRIVAS
                     FEGVKQLYRYYSHIYTILSYPWLIRKEFPKAQIMIGGGAFTAFADQLIEKLPEGTIGL
                     LGEGEDAILKVIEGRSIEDERYIVKEGKRIRKGQQGSPALLDALTVDLPYLTSIFPQY
                     REFQGEAIGVQTKRGCPYDCAFCLYPYIEGKRVRYRPPEMVVKDVSQHYHQWGTRRFW
                     FTDAQFITGKEAYPQCTEILERIISEKMEIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VESYKMVAAILGEERVFPLMFFLGIQPNTDLEHRLLEEGYLSAGYNPLMLTPTSIRKL
                     LYNPAPLNKIIAKACLKAWERKNGSRDPRKWSGSLSQTPTEKETYADANLIRGIEGNS
                     GRDALLSLEEILRTRKSLKPSTDSEQKPLTSSVG"
     gene            complement(27025..27777)
                     /locus_tag="VLD60_13555"
     CDS             complement(27025..27777)
                     /locus_tag="VLD60_13555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB46035.1"
                     /translation="MAQNSPTPPQAVSTWSGQAREQAVQRMFTAIARCYDLNNTLLSF
                     GLHYRWKKLTASFVPVVERGTALDVGAGTADLALLVEPRMGAKGRVVASDLNHAMLRE
                     GLKKVDRKGLTGKITCLQANAEQLGFADNIFDAVTTGFCMRNVGNLPQAVSEIRRVMK
                     PGGRFVCLEFSRPVFGWLRALYDWYSFTLLPWIGTKVARDTTGVYEYLPASIRTFPDQ
                     ERLCQVLREAGFQQVSYKNLTGGIVAIHVAVK"
BASE COUNT         5781 a         7622 c         8417 g         6125 t
ORIGIN      
        1 cctgcgaccc tcttagatct tcatggtcag ccgatccctt cccactgtga tcggtccggt
       61 gaacgattct cctgcctgct ccttcacgtt ataccgttcc gcaatcggat aaaaatgcga
      121 gagcaccaga cggccgattc cagcctccat cgctacctgc ccgcattcgc ctgcgtggag
      181 atgcgccggg cctggtttgt ttgcagggaa cgagcagtcg agtacggcga catccacatt
      241 tcgacacaga tggaccagat tgtcgcaata ctgcgaatct cccgagtagg ccagcgcctt
      301 cccgcgatag tcgatccgat agcctatgca atggagatcc ggcacatgtg tcacagttcg
      361 cggagagatt cgcgtctctc cgagattaaa acaccggtcc cccacttcat tgaagatcac
      421 gcgaaacggg ggccgggaaa aactcgggaa cgtcttcatg atcgtcttca tcaagcgctt
      481 cgaccccttt ggtccgatca ccgttaggtc ggggcgatgc ccccccccgt acttggcata
      541 gatcacggcg tcgaaaaaga acgtgatgaa atcggagaag tggtccgcgt ggaagtgaga
      601 aaagagaatg tgcgtgatcc ggtggcggtt gacgccggtt ttgataagat tgctcaaggt
      661 gcgcggtcca aaatccaata gcagcgtctg gtccgtacgc accagatagc cggcagccgc
      721 gcgacttgga tgaacgttcg tcccggaccc tagaatggtc aattgcatcg taggatggtc
      781 tctaccggtt ctgcgcgtga aaactatccc gcaagcgaag caaaagggta cgggcgatct
      841 ctgcgtcgtt cgggctcaac gctcgttcga aaaacggtcg aagaaaagca atgtggccgg
      901 catagacttt cctgaagagg gcgttgcctt tttcagttaa acaaattttc gtgcgccgct
      961 tgtccgcttt aacggactct cgccggatca accctttctt cgccagacga tccagcaccc
     1021 cagtcaacgt tccttttgtc acgagcgtct gagccgacaa ttcggagcag gtgagtccgt
     1081 tcgtatcacc gagcgtcacg atcacgtcga actgcgaagg agtcagccgc atcgatcgaa
     1141 tgtggcggct atcgacgcgc cagaatgcca gataggcctc ggccagaggt cgaaccactt
     1201 tgagataggg atcatccttg aaatccggga ggtccatttg aagggggaga atagtccgcg
     1261 ctagaaccac cggtcaagcc gggtggacgg gctgcctggt cctcctcctg ctcgcggaac
     1321 gcgcacgcca gacttatcga atggtgacat caagggcggt gctcgttcga cgcgcgcatt
     1381 cgaggagaga ccagacatcc ttataggaat aaaagtgaaa acctccaaag aggccccgtc
     1441 aagatttctc ttgctcgttg ccagtaagtc ctggcatctc gaccgtccca ttgcgtcttt
     1501 ccgttgacgt cgcggcggct gcctccctat gatgcgatcc ctatgctgcg acgctcgtcc
     1561 ggcaacgcca acctgaccgc cattgccctg cttctggggg tggtcattgc cgccgtgtgg
     1621 gtgtggaagc ggctttccct cgagacgcag gaatatatca tcgatcaagc cattcccctg
     1681 gtgtttgtgg gcgcgctctg ttgtgcggtg ctgtggacgg tggtgcgagc tgtcaggaag
     1741 cggaagcgcc gtctcagtca gcgcgcgaag ctcctcgcag catttgaacg agaaaccgcg
     1801 agggatagga aactcgagac ggcattcgcc ttgatcgaag tgaacgagta ccgtgtgcag
     1861 ggactcgaat ccgtggttcc ggcgatgaga gaattgttcg tcacgacgtt gcagcgtgcg
     1921 gtaggcgaga agcagcacag gattcgcggg atggcggcca gccaccttgg agtgcttgaa
     1981 gatatgacgg tggtgccgtt gctggtcaaa gcgttggaag acgatcacgc ctacgtccgt
     2041 tcttgtgcgg ccttgggatt ggggcggctg cgggcggtgg aaacgcggga gcgattgaaa
     2101 gtgttgatgg aggaagattg ggatcagaca gtgagaagtc gggcgaaaga agcgttggag
     2161 cggatgagtg cctgagggtt atgctgcccg cgcctgcgtc tgttcatttg tccaatcgat
     2221 gacggcgatt tccggctcgc aattccatcg gaacggaaga aacgaccatc ccattccccg
     2281 gttgatgtaa agcgtacatt caccagaccg atgttcgcct tcgatgcggc cgttacagcc
     2341 gggcggaagc cagaatgtgg ggatcccggg aatgcgccac tgcccgccgt ggctgtgccc
     2401 gcagagaatc aagtcgacga cgtgctcctc ctccatctga tcgagaatat tcggcgcatg
     2461 cgcgaggagt aacgtaaacc gacggtctgc aggaggcggt agacagctga gatcggcacg
     2521 atgcaatgaa ggatcgtcga cccctgcgag ggccagttcc cccgtgcctg tttgaatgaa
     2581 cgtggtctgg ttcaccagga gcgtgatgcc gtgccgatcg gcaagttcgg catactgctc
     2641 gacggacacc ccactgtagt gatcgtggtt gccgagggtg atatagacgg gggccacttc
     2701 gcgaaggcgg gcgagaaagc ggaacagatg agggagtcct tccggcacgt tgagcaggtc
     2761 gccggtgatg aaaatccaat caggattgag ttcgtcgacg gcttcaacga cacgctcatg
     2821 gcgcggatgg tagcggtcga gatgcaggtc gctcaagtgc acggcccggc gccccgccag
     2881 cgctccgtgt gtaaaggtca ggttggacac cctgtggctg gacagaccga attcccaatg
     2941 gggtacgagg ctgaagagac gataaagcgg ttcgctcagg cagtagccga taaaggaacg
     3001 gagccgatcg ggccaggaca tccgccgtcg cgtcatttca tgacccggtc caactcggct
     3061 cgatcgttgg gggtgacgcg ataccctcgc tcgtagaggg tgctcatgcc ctgcatgacc
     3121 tggtcaagcg ttctggcgcg ataccaattg tcgaatcgat ccgtcaaggt gtcgatggaa
     3181 gcccgcggcg aatccgctgt atcgtaggcc agggagaggg actggaaaag atcctgcaac
     3241 tcgatggtgc tgtaggattg attgtcatct tgatcggcga ccaaggcaaa ttcgtagatt
     3301 gtgaggcaga cggacaacaa cgtttgcgtt ttggcgaaat cctctcgcgc cgcatccata
     3361 ccgccaggat agatcctggg gcactcttct tcgagtggcg gtgttgcggt cgggctccaa
     3421 ttgatgccct gagccgcagg cggcggcggc ttcttcttgg atgctttgta gaaccaggac
     3481 aaacatgtgc tggcttgggc gaaggcctgt ttgtccagac ccatttgacg ggtaatatgc
     3541 ccggtgaacg acgaaaagac ggcgttgtct attcgccctg cactcctggc ctcttctggg
     3601 gcgatggcca gtaggagaat gaagaccgtt ccgacaacta tgtttaatcc gggatgcatc
     3661 gaaaaagtat accatgacgc aggccaacgc ccgatgaaag attaagattg tgcctgcttg
     3721 cagcgacgac agaatggatc gcattccaac cacgcagccc tatctcacga gcgccatgcc
     3781 aggcatcggc ggccgcatgc gtgtggagcc ggaggatttt cgagtcgacg agcgcccgtt
     3841 gtatctgcct tgcggagaag gggaacatct ttatatcaag gtcaccaagc ggctactgtc
     3901 gacgccggat ctgattcgga agatttcgtc aacggtgggt gtcaaagcgc agggaattgg
     3961 cacagccgga ctcaaggatg cgagagccgt cacgactcag atggtgtctt tgcatggggt
     4021 cacccccgac cggttggccc ggttgaaagc ggatgggcag atcctgtcaa ttgagctcct
     4081 ggggcgacat cgcaatcgct tgcgtccagg ccatcatgca ggcaatcaat tccggcttgt
     4141 cattcgagac gtggctgccc atgcgaagga gacggttccc tcggtgctgg atgtcttggc
     4201 gcgccgtgga gtgccaaatt atttcggacc ccaacggcaa ggacggacag gagagaacta
     4261 tcagaccggg gtggcgcttc tcacggattc ggatcggaga aacaagatga gccgcgccaa
     4321 gcggatgtgg tacctcaacg cctttcaatc acacctgttc aatcaaatcc tggcccgccg
     4381 catcgatcgc atcgatcgca tcctggccgg tgattgggcg atgaagatgg aaaacggagc
     4441 ctgtttcctc gtcgacaatg ccgaaaccga acagcctcgc gcggatcgat tcgaaatcag
     4501 ccccaccggc attctgtttg gatcgcgcgt gtcctgggcg agcggagaac cgggcgaaat
     4561 cgaacgggct gtcgcgacgg gggcgggtgc cacacctgag cgcctgactg aggcagccaa
     4621 ggcctgcggg ttccgcggag agcgtcgatc gctgcgagtc ccattagcgg atctcgactg
     4681 gtcactggat ggctccatcc ttaccctctc tttctctctg ccgccgggcg cgtatgccac
     4741 gagtgtcttg cgggagttga tgaaagcccc tgaaccatct gttcgctaga cgcatcttct
     4801 tcttcgactc catgtatatg gatataagcc ttgccgaccc gaccttcacg gaaaaggagt
     4861 ggtaaaattt acctagcagt cgggagttaa gagggtggtg ccatggtgta tcaagagacc
     4921 gtgcttcttc aaggccacat catcgattca ttaattctgg caaaggtgct ggatcgaatc
     4981 ctttcaatgg gtggctcgtt cgacctgcaa gaggttcaga tcggaaagac gcgtgaggat
     5041 ccctctcggg ccaagattgt ggtgcaaaca gaatcccctg cgcagctcaa atccatcctg
     5101 gaagcgattc agccccatgg cgcttcggtg gaacgcgaat cggattgcca attccagccg
     5161 gcgccggggg acggggtctt ccccgatgag ttttatgcga ccacccatct gccgacgcag
     5221 gtgcggctgc ggggatcgtg gatcaatgtg gatctgatcg aaatggatct gggaatcgtg
     5281 gtcgagcctg aacgaggcgt ggccagagcc gtgccgatgg cctcggtgaa gggcggcgag
     5341 tccgttgtaa tcggccggca aggcgtgcgg gtcattcccc ttcagcggcc gcgcaagcgc
     5401 gacatcttca gcttcatgga gtcgcaggtg tcagtcgagc gaccgcacgg gcacatgatc
     5461 cacgatatcg ccacacgcat gaggcaactg cggtcaggat atcgcgaagg acaagccgat
     5521 agccggatcc tgttctcggg aggaccggcg atcgttcagg ctggtggtca ggatgccttg
     5581 gcctggctga tcgagaacga gtatgtccaa gtgttgttct gcgggaatgc actggcggca
     5641 catgatctgg aaatggagct ctacggcaca tcgctgggtt atgcgcatca ggccggccag
     5701 ccgcttcccc atgggcacga acatcacttg cgaacgatca atcgcatccg atcaatcggg
     5761 agtattaaga aggccgtgga cgccgggttg gtgaagcagg gtatcatggc tgcttgcgtg
     5821 agacaggctg tgcaagtcgt gatggcggga acgatcaggg acgatggacc gcttccgggt
     5881 gtgatcacgg attcgattca ggctcaagaa gccatgcgga aagcgattcc aggaaccggc
     5941 ttggccttac tggttgcttc gacgttgcac gccgtggcga ccggaaacct gttgccggcg
     6001 tcggtcccga ccgtgtgcgt ggacatcaat ccctctgtcc cgacaaaact tgcagatcga
     6061 ggaagttttc aagcagttgg acttgtgatg gacgccgcgt cattcctgcg cgagatcgcg
     6121 cgtgaattgg ggtggaagcc atgagccggc tgctcgtctg tcccccggac ttcttcggta
     6181 tcgaatacga aatcaatcca tggatgcatc gctccaatgc cgtgaatcgt gagcgcgcga
     6241 ctcggcagtg gcgtcggtta atgcaggtct tggaacagga cgtgggagtg ggccttgaac
     6301 ggatgaggcc tgtccaagga ttgccggatc tcgtgtttac agcgaacgcg ggagtggtag
     6361 tggggcggca ggccgtcccg agccgattcc gctatcccga acggcagcgt gaagaggcgc
     6421 agttcgaaga ttggtttcgt ctgcatggct atgagattct cacgcttgag cgggatcatc
     6481 atttcgaagg tgccggcgat ctgctgggat ttcaggactg ttggttcggc ggctataggc
     6541 agcggacgga cattcgagcc tttccgctcc tgagtgaatg gtttcatcgg gagatcatcc
     6601 cactggagct ggtggataac cgcttctacc atttggatac ctgtttttgt cccctgagcg
     6661 gcggcgaact gctctatttc ccgcccgcct tcgaccgcta cggtcaggaa gcgattgcgg
     6721 accgaatcga cccgatgcaa cgactcgccg ttcctgaacg cgaagccctc gccttcgcct
     6781 gcaatgcagt gtgcatcggg aagcatgtcg tgctgcccgc cgattgtccc gacacggagg
     6841 cgtggcttgt ctcgcgcggg taccggcccc atgccgttcc ccttgatgaa tttctcaagt
     6901 ccggcggatc ggcgaaatgc ctcacgctgg cgttggattg agagaccttc cgccaagccg
     6961 actagcgact tcgaaaaaga aatgatccgt agagaccggc gatggcgatc gtcaccagtt
     7021 cgatgctgat caggagccgg ctgacgacgg cctcgggagt tggcggcgaa agaatgaagt
     7081 gcagtccgag aaattcagga gcttgcgacg gcggcgtttc ccacggcggg aacagcaagg
     7141 cgaggacgag catcgctacc atgccataga gaatccggag attaagcgcc tccggggtgg
     7201 cgtccggttt ggctgatgta ggctgaggtc tgccatccag gctgctgccg cactcggtgc
     7261 agtaacgata gatctcagga agttcacgct gacaggatgg acagattttc atacgcggtc
     7321 cgtgcctagc ctacacgaaa cggcgcgtag tgtaccagat ctgatggggg agaagcttcc
     7381 ggctagaagt gataactgac accgatactt acggtgaaag cactgtatgt cgcgcgaaat
     7441 ccaaacggat ctgcagcagg gttactatca ttggaactgt aactaagccg ggctctgttg
     7501 tacacgcctt ccccaaagag ggcccagtgt cgagtaataa agtagcgcgc ccctgcttcg
     7561 gcgttcagtc ctattgcagt tgccgattgt ccgggagggg ctgtgggacc attgagactg
     7621 gcaaagaaga gcgctggacc aacgccgaca tagggttgga agcgattgcc gggataacgc
     7681 agcatgaaag ttaccacatc ccatatgatg atgcgttggt gcacccctga gagggtgcct
     7741 gaagtgagag accctccggg ccctgtaaat gtcagcgacc cctctttgat atgaggcgtc
     7801 gtataggaca aacccgtttc aattccgacc catctggctt tggagaaata atgcccgagc
     7861 tttgcaccaa cgttgacgga actttttagc gactgatctg aaatatcaag accgccaaat
     7921 ccatcttggg tcaccacgcc gttgcttaag ctttgaggca tggtcacccc gaatgttcct
     7981 gcgacatacg actccgcctc cgtcatcgat ggtaagagga tcgcgtgaaa catgatggtg
     8041 aggaagatag ctccccggac tctgttgatc gtgcgtttta tgttcattat ctagctcccg
     8101 ctgcgacgcg aaacgcccac cggtatccta tcggccttcc aggcatggac gaagcgattt
     8161 gtgaaattct agagtggatg agcaagccat ataccacctg aaatgcggaa aagcatgcct
     8221 atttcgcgat ttcccggcgg tgaactcatt cggctgtcca ctcaagcaag cgggattgta
     8281 cggttcctgc acaggcatgt tcattgacag gagaaagtac cccttcctat aatccgcccg
     8341 cccgatcatt cgtttacgtg tcgaattgat gtgcccccgt ctctacgtag ctcttcgtac
     8401 aacaccatgc cgaccatcca tcgaatagga gtcataggag caggggcctg gggaacagcc
     8461 ctggcaaaac atttggcgga gaaaggtctc cacaccactc tttgggctta cgagagggat
     8521 gtggtcgatt caatcactgc caaacgggag aaccagttgt ttttgcccgg agtcaccctg
     8581 ccgtcagcac tcgtcgttac gaatacgttg gtcgaagcca tagagggcag tgacggcctg
     8641 ttattcgtcg tgccgtcgca tgtggcccgt caggtactcc ggcaggttgc accccttcta
     8701 tccagttcca cgcccatcgt gagtgcgacg aagggtgtcg aagaagacac gttaaagctg
     8761 atgacgcaag ttatggagga tgtgctgtct cctccactcc atgatcgact gatggtgtta
     8821 tcggggccaa gttttgcgac ggaagtcagc cagggacaac cgacggctct ctgtctggca
     8881 gggaaggacg gtttgctcgt cgaagcgttt caaaccgtct tcatgacatc ggctttgcga
     8941 atctatgcag acagcgatgt catcggcgtg caattgggcg gggcgttgaa gaacgtgatg
     9001 gcgttggcgg cgggagtagt tgatgggctt gggctcgggc acaacacacg cgccgcgttg
     9061 attacgagag gtctggccga aatggtccgt ctcgggaatg cgatgggcgc ggacccgcgg
     9121 accttctatg ggttatcagg cgtcggagat ctggtgctga cctgcacagg agcgttaagc
     9181 cgcaaccata cggttggcgt tcgtctcggg aaaggcgagt cactcgagac gatactcggt
     9241 ggtatgcatg ctgtggccga aggaatacgg accagccggg cggccgcagg cttggcgctt
     9301 cgtcatagag tggaaatgcc gatcgtgcgt gagatcaacg cggtgctctt cgagggcaag
     9361 tcttgcagga aagcggtcgg cgatctcatg gagcgggttg ccaagccgga gaaagggcag
     9421 acatgaatcc ggcgcgcctc gaacgtttgc tgaccaatgt gcggagcggc accctgtcag
     9481 tgaccaaggc tttagaacaa ctccgatcgc tgccgttcga ggatctgggg tttgcgtcgc
     9541 ttgatcatca ccggtcgctc cgccagggat ttccggaggt gatcttgtgt gagggaaaga
     9601 cactctcaca gatacagagg atcgccaagg cactgctcaa acatcaccgg ccatttctgg
     9661 ccacgagagc cacacgccag gttgcttcgc tgatcaaacg cattgatggc cgggcgcagt
     9721 accacgaaga cgcacggctc gtggcaattc gcgatcccaa gcgaccgctt caggggcatg
     9781 tcttggtgat tacggccgga acctccgacg ttccggtcgc agaagaagcg cgcatcagcg
     9841 cggaggtcat gggcagccac gtcgagacgc tctatgatgt cggtgttgcc ggcattcatc
     9901 gactattgga tcgtcaccat cgactgaccg aggccaacgt gatcgtggtc gtcgctggga
     9961 tggacggggt gttgccgagc gttgtcggtg ggttggtgga tgtgccggtg gtggcggtcc
    10021 cgacgagtcg aggctacggc gcgagtttcg gcggactggc cgcgttgctg acgatgctga
    10081 attcctgtgc ggccggcgtg ggggtcatga acatcgacaa cgggttcggc gctggctgtc
    10141 ttgcacaccg catcaactcg ttaggggtga agcgcaacga gcgggtgatg cgcgacaagc
    10201 gcgaaaggtg agatgctgaa cgagtgagga ttcctacctc tcccgccagt cgcgccttct
    10261 cgcgcgtccc gccgcagatt acttcacttc caaggttccg cgtttcggcc aagacaccat
    10321 cagtgtacgg ggcccctttt caccgttcgt cagaagtttg gcccgcaatt cgtactggta
    10381 aagtcccgcc tcggccggtg ttttcttgtg atccattcca tcccatgcca ggacgacgga
    10441 gatcttcggg cggtcagcgc tgttctcggg ggtggaatgc ggttccagtg gtttgcgtat
    10501 tgagaggaaa cggatcgaag ttttcgaggg agaagagacg agcgacgaca cttctaacag
    10561 tgttgcgccg ttcaactcca tcgggagttg aaccagagct gagaattcca gggagcccgc
    10621 tgaaacggtg taggggttcg ggctgacctt gagttccaga accttcagtt ccggctcagg
    10681 gcgaggggcc ctgttcggct tggtctttgc cgatgaggcg gctggtagca ggagcgcgat
    10741 cgccaggatg gcaacggtcc atcgccaatg atgatgccga acgcggaaat ctgtcgatgg
    10801 agatgagctg gagagagctg tgaagaacat ccggctgtct agaggcctta cggttgtcag
    10861 caggcgtgag gaaccaattg cagcagtggg gataacatag cgttctccgg ctgtcaatgc
    10921 attgccgttc tgaccggcct tgggtaagat ggcgcggcct gatgaggcga gaggaagaag
    10981 atcaatgagc ctgcatctcc attttgactg cttttccgga atcagcggag acatgacgct
    11041 tggcgcgctc gtggacgccg gtgcgccctt tccggatctg gtaaaggggc tcaagcgact
    11101 gggtgtggca ggattcacat tgcgaaaacg gcgtgttcag cgcgggggga tttccgcgac
    11161 caaagttgat gtggtgatcg gggccggcct gcgtcatccg ctatcgctcc cacgcattcg
    11221 caagatcctg tctgccagtc gcctttcacc gaagatcaag gagcaaagcc gcgccgtgtt
    11281 cgaacgtctc gcggaggctg aagggaaggc ccatcgcgtt gcgaaggatc atgtgcattt
    11341 ccatgaagtc ggcgtcttgg attcgttcgt tgatatcatc ggcgggttga tctgctgcga
    11401 actgttgggc gtcaccagag tgaccgcatc tcccgtgaat gtgggggcgg ggacgctccg
    11461 atcggcccac ggcattctac cagcgcccgg acctgccgtg gccattttgg cgaaagggct
    11521 tcccatctac agcgccggtc catcccgcga actgacgacg ccaaccggtc ttgcgctgtt
    11581 gcagacgctg acgaccgagt tcgggcccat gccattgctg cagccgacat ctgtcggata
    11641 cggggctggt gatgcgaatc cggcaggctg gccgaatgtg ctgcgcgtgt ttctctcgcg
    11701 cgattcgtcg cgagggggca gggggcgcga tacagtcttg cagatcgaga cgaatctcga
    11761 cgatctcaac cctcaaacgt atgagcatat tatggagcgg ttgttccgct ccggggcgct
    11821 cgatgtcacg ctgacccccg tcatcatgaa acgggggagg cccgggatcg tgctgacctg
    11881 cctcgttgtg cccaccggcc tccatccgct gctcaacgtg ctgtttgaag agacaacagc
    11941 gttgggcgtt cgggtacaag aagtcggacg gcacattctt ccgcggcgat ttacgtcggt
    12001 gaagattcgt ggcggtgagg ttcgggtgaa aatcgcggca gtggatgaaa cggtgacgaa
    12061 agctgccccc gaatatgcgg actgccaacg catcgcgcag cggaccggcc ggccggtcaa
    12121 agcggttctc gaggaagcag cgctcgccta tgccaagggg cgctggcccg cgaaggacca
    12181 ttcgtgaccc tcctgctcta tagtctcttg ttcatcgcat ccgtcgccgt cacgctcggt
    12241 gcctgcaccc tctttacgaa cgcgattgaa tggctgggga agcgactgaa tctctcggag
    12301 ggagcggtcg gcagcgtctt ggccgctatc ggcacgacct tacctgaaac ctcgattcct
    12361 attgtggcga ttttcttcgg aaccagtcgt caggaggccg aagtgggtgt cggtgcgatc
    12421 ctcggcgcac cgttcatgct tagcaccctt gtggtgccga ttttggccct gctccttctc
    12481 ctcttctcca gactggggaa gcggagcccg ttgtttcggc tgaactataa agatgtcacg
    12541 gtcgatctct cgttttttgc gttcgggtac acggtggcca tggcctgcgc ctttatccct
    12601 tctcgtctct tgcacgtgat cgcggcgatc ggactcatga cctgctatct ctactatgtg
    12661 aaactgaagt tcagcgattc gggtgaagaa ggcgatggcg gcgagcttga tcccttattc
    12721 ttcttcggcc ggaaatcagc gacgccctct tacggtctga tcgcgttgca ggcgatcgcc
    12781 ggtcttgcag gacttgctct gggcgcacat ctctttgtga cggccgccga atcgacggca
    12841 cagtttctcg ggatttctcc tttgattctg gcgctgctca tcgcccctct tgcgacggag
    12901 ttaccggaaa tgtcgaacag cttcttgtgg ctctaccaaa agaaagatcg cctggcagta
    12961 ggcaatgtga ccggtgccat ggtgtttcag ggcatgtttc ctgtgtcgat cgggttattg
    13021 gggacggatt ggaccttggc tcccaacgcc ttgatcacca tggtgctcgc cgtggtggcg
    13081 gctggcagtt tgctcatcca aacagcctgg gcaggccatt ggcggccctg gttgctgagc
    13141 gccggtgcgg tgctttatat aggttatgcg ttgtatttgt acgggtcttg aacagcggtc
    13201 tacagccggc agacattaat gggtaccaag aacaatcggg tgatacagaa gagcaagccg
    13261 cttctcggca tcacgatggg tgatccggcc gggatcggcc cggaagtgat cgccaaggca
    13321 ttgtccggga ccaagttgag acgtgtctgt tttccgctcg tcatcgggtc gctgcccgtc
    13381 atgcagcaga cagtcaagaa gctgcgattg aaactggatg tccgatccgt cgacggtcat
    13441 gaccggaatg cgctccgcgc gaaccagatc gccgtgttgg atcccctgga gaagcccctt
    13501 ggatcattta aagttggagt tgcggctcct gaaacagggg cggcctcagt cgccttcatc
    13561 aagaaggccg ttcacctggc tcaaatcggt tgtgtcgatg gcatggttac ggcgccgatc
    13621 aacaaggaag ccatcaacat ggccgggtgt cactttcccg gccatacaga actgctggcc
    13681 gagctgaccc atactgagga atcgggcatg atgatcatcg gtgggccgct ccgcatcatg
    13741 ttcgtcacga cccatgtggc gatcaaggat cttccgtcgc tgcttaccca ggacaagatc
    13801 gaaaggggaa tccggctggc gcatctcgca ttgacgacgc tcttcgggat caagaaaccg
    13861 agaatcggcc tggctgcatt gaatccccat gcaggtgagc acgggctctt tggagatgaa
    13921 gaagcgcacg tcgtccttcc ggcggcacgc gcagctcagg cgcaggggat tcttgccagc
    13981 aatccgctgc ccgccgatag cctgtttggg aaagcggccc ggggtgatta cgacggcgtc
    14041 gtggcgctct accacgatca gggattgata cctctcaagt tggtcgcgtt cgggacctgc
    14101 gtgaacctca cagtcgggct tccgatcatt cgaacatcgg tcgatcatgg caccgcattc
    14161 gatattgttg ggaaagggat cgccgatcca ggcagcttga tcgaagccgt ctcgctcgcg
    14221 gccgaactgg cgacgaaacg gcaatcacgg aaggctatct agcaggagtg gatggggaga
    14281 tgtcgcagat gacgaacaaa gaacaggcag tcgttcagca gcaattgaag gaattggacc
    14341 agatctttca ggacacgatg gagaccctca atacggtggc gggaatggag cgggtcgcca
    14401 agtggaaagc caagaccgtg gcattgctga gcgaagcggt cggtcaacag gccggtcagg
    14461 atttcgcgcg tgtccagcct ggtccgtcat ttaccaacga cctcgttgaa gagttctccg
    14521 acttcatcga ctgtttcaaa acgccgcttt cagccctctc caagaagttg tctcaggccg
    14581 gccctcgtcc atccggcgga ggctgagtgg tgccctctcc cacctttccc cccgccaaca
    14641 aacggctcgg tcagaatttc ctcatcgatc ccaacatcgt gcgcaagatc attgcgctgg
    14701 ctgagctcac acctcaatcg gcggtccttg aaatcggccc gggaaggggg attctgacag
    14761 aggcgctttc ccgtgatgcg ggacaggttg tcgcgatcga aattgacccg gtcctccacc
    14821 gcttgctgca agaacggcag gcagattggc ccaacgtcga attggtttgt ggcgatgcgc
    14881 tggcctatcc ggttgagaat ctgccgaccg gcacagtcgt cgtcgccaat ctcccctatt
    14941 atatttcgac cccgctcttg ttcagactgc tcgattgccg ctcccggttt cccaggatgg
    15001 tgctcatgct gcaagacgaa gttgccgacc gtctcgtagc gaaaccggga agttccgact
    15061 atggcgtcct ttccgtgatg gctcagtatg ctgccgatat caccaaggcc ttcaaggtct
    15121 cggcgcaatg tttcaggcct cgcccggagg ttggctcagc cgtggtcctc ctacgcagca
    15181 aagcgcaggt gctgcttcga ccagaggaaa aaccggcgtt cgcagcgctt gtgaaagcct
    15241 cgtttgccca tcggcgcaag atgctcgtca actcgctccg cgacgaagac tatgatcaac
    15301 agctggtcgc caacgcgctg aacgacatgg gcattcccct cacgatccgg gccgaaacgt
    15361 tagcgctcga tcagttcatg gagctgacaa aacggctggc cgtttaagag catgcccgga
    15421 acgggcggat gacgactccc cgcgtttgaa ttgactttgc ccctgtgtta gcatccgctt
    15481 catgggcgta accagctcgg ccaagcgggg cgaggcccgc cgcgcccctg ctcccccttc
    15541 ccacatcagg cgtgaagtca tcggcgtgat cttgatcgcg ctgagcctgc tcacactcct
    15601 gagcttgctg tcatttgtcc ccggcgagcc gaaagtcgtg gccacaccgg ggtcgacagg
    15661 ttcgcctcca acgcacaatt tgatcggatc ggtcggggcc gtgttctcat ccgtgctctt
    15721 ttcgttgatc ggcggggccg cctacctgtt cccgatcctg ctcggtttga tgggcatccg
    15781 ctgttttacc cagagcgacc tttccattcg tttacggaat gctggtgcct cccttggagc
    15841 cctattattc ctcagcgcct tcctccatct ggaagtgatg gctgtgccga cactgtcgag
    15901 cgggttggtg ttccgcgggc aggcaggcgg cctattcggg caaattctcg ccgaagggtt
    15961 gcgggtgtat tttgccagca ccggcgcgca tattttgatc atcgcagggt tcctcgtcgc
    16021 cctcctcttt acgaccccct tatcgctggc gcaaatcgcg atccaagtgc ctgactgggg
    16081 gcgtaagctc ttagaagcgg cccgtgcgct gatccccgaa cgaacgaatg aagagccggt
    16141 cacggagcct tcgaaggtat ccaagccgaa aaaagcgaag tctatccgag cagtcatcga
    16201 agaggtcatt ccgcagccgg tagagacacc gcctgccgta gagccggtca ttcagccgca
    16261 gctcgttgct gtgcctgaac agccggatgt cgctgaattt gagcctgaag gaatggtgag
    16321 cgggaaaatc gaagatccgg atgattatca actgccggat ccggacgaac tgttgagcga
    16381 gccgtccggc gtactatccc gtgtcacgga tgaggagttg aagacacaat cggagattct
    16441 ctccaaagcg ctgttgagtt tcgggatcga gggcaaggtg acggaggtcc gacctggtcc
    16501 ggtggtcaca atgtacgagt tcgaaccggc accagggaca aaagtggcgc gcatcgtgaa
    16561 tttagccgac gatctggcgt tggcgatgaa ggccatcagt ctgcgtatcg tcgcgcccat
    16621 tccggggaag tctgtggttg ggatcgaagt gccgaatgcc caacgggaaa tggtttcgat
    16681 gaaagaagtg gcgaccagcg aagcctttac cagggcgcga tccaaactca cccttgccct
    16741 cggtaaggat atttttgggg cccccgtgat cgccgatctc aaaaccatgc cacacttact
    16801 cgtcgccggt gcgaccgggg caggaaagag cgtcggcttg aatacgatgt tgatcagcat
    16861 cttactgtcc gccaggccga atgaggtgaa gctgctgctc atcgatccga agatgctgga
    16921 gttccagacc tatgacggca ttccacatct cctgcggcct gtcattaccg atccgaaatc
    16981 agcggcgcgc ggcttgagtt gggtggttca ggaaatggaa cgccgctata aactgttagc
    17041 cgaggccggg gtgcgcaaca tcgattcgta taatcgaaaa gtcgccggtg ctcagggggt
    17101 atcaggtgac agcggaacgg agacgaagcc ggatcaaccc gaattgccga tgcagtttct
    17161 ttcagaggaa gagcggcttt cagccgggga gacgattatt ccggaaggca gtccaggctc
    17221 gttcgctccg cctccgacac cgccggaacc cttgccctac attgtcgtca tgatcgacga
    17281 gttggccgac ttgatgatgg tggcgccaaa agacgtcgaa gacaagatcg cccgcttggc
    17341 gcaaatggcc agggcctcgg gcatccatct cgtgctggcc acgcaacggc cgtcggtcga
    17401 cgtattgacc gggttgatca aggcaaattt tccggcccgc atcgcgtttc aagtctcatc
    17461 gaagacggac tcccgtacca tcctggatgc caatggggcc gaagcattgc tggggcgcgg
    17521 ggacatgctc tatctggcct cgggaaccgg gcgtctgatg cggctgcacg gttcgttcgt
    17581 ctccgacgac gatgtccgtc gggttgtcga atttatcaag gaacaggctc tgcccagtta
    17641 taaccaagag ctgcagtcct tgaaacagga agaggccaag gaggaagagg cgcaagacga
    17701 agtctatgaa caggccaagg atttggtcct gtcgacgggg caggcgtcgg cctcgttgat
    17761 tcagcgccgt cttcgggtcg gatatcctcg tgcagcaagg atgatcgaac agatggaagc
    17821 agatgggctc gtcggcgcgg ccggtcggga cggccgtcga gaagtcatcg gacggcgtgg
    17881 accggtaggg gatgcgcaag gatgaaccat ttctggatcc atggcgccgt cctcctggcg
    17941 ctcactctca gctccgtggc cttggcagaa gatgcggtag acgagcaggc gcggaaagaa
    18001 gtacgtgagg tggtcaaacg attgcaggcg cgctatgaga agaccaaaga tctccaggcg
    18061 gatttcacgc aaaagaccac gatcgaagga ttcgaacggt ccatgaattc ttcggggaag
    18121 gtctatatca aaaagccggg ccgtttgcgc tggaactacc tcgacccttc gacggaagat
    18181 atctacgtcc aaggggacga cgtgaaagtc tatgtgcctg agcataaaca agtgttggtt
    18241 ggaaagctca cccagatggc tgcgtcgaag gccccgctgg aactcctgca gggcgcggcg
    18301 aagctggaag agtcctttga cgtggagccg acgccaggga agggacgggg ggtcggaggt
    18361 atccgactcc tgacgctgct accaaaatct cgcgagggtg aggcgcaccg gacacttcaa
    18421 aagattgtgg tggaagtgtt tccgaagacc tattacattc gtacggtctc tctctatgag
    18481 atcagcggta atgtctcgaa tttcgagttt tcctcgttgc agacgaacgt tgggcttggc
    18541 gacgacgtct tcaacccgaa gctgccagca gatgtcgaag tcgtgaaggc gcccgtcttg
    18601 aaccagccat gatagcgggc acggacatat tctcaacgga gccggaggag cagcggatat
    18661 gagtactgga gtgttgagcc aggttgctga agcgacggat caagccgttg ccaggctgga
    18721 tgttgatcga ttacatcgag agtactggga gcagaatgag ttcctgttca ttccgcaatt
    18781 tcttccgcgc gcgtttgtcg aagcaaccct tgtcccgcaa gcccaggggg tcaagggaga
    18841 tttgaatcgc aattttattc cgggccacaa gaagggaggg agcgtcagct actatactgt
    18901 gcaagagaaa gccccgctct ttcttgattt ataccgctcg cctgcgttcc tgaatttcct
    18961 cagccggttg gtcgaggcca aactcatgct ttgcccagat aacgatcccc attcctgcgc
    19021 gctctactac tacactgaac cgggcgatca tatcgggttt cactacgata cttcctacta
    19081 taagggggcc cgttacacga tcctcatggg actcgtcgac aagtccactc aatgcaaact
    19141 ggtctgcgaa ctgttcaaag atgatcccgt gaagacacct cgaaagttgg agctggtgac
    19201 gcaaccaggt gatctggtga tctttaacgg ggataaactc tggcatgcag tcacgccgct
    19261 cggccagggc gaagagcgga ttgccttgac catggaatat gtcacgaatc cagagatggg
    19321 accgttcaag cgactctatt ccaacctcaa agactccttt gcctactttg gaattcgagg
    19381 tgtgttcaag cgggcctggt ccgcttcaca tgagtgacgc tgaaatctct gcccctcgtc
    19441 tcagattcac atatgctagg gagtgtccga ggttccacgc ggacctaccg tactccattc
    19501 agcctggcct ccgatccggt cggctcatga attgtacgat tgtcggtggg tccgttgttg
    19561 gcagtcgttc ggatcgcctg acgaactaac aggcggagct cgttgtgatc gtagggtttc
    19621 gagaggaagg tgaaggcgcc ttgagcgagt acgtcggcgt acaagtcggt cgaagtggct
    19681 gctgtgagca tgatgacaga gagagacgga acactttcct ggaaatggtt gaggaccgac
    19741 aagccatccc cgtccggtag tccgatatca agcagcacag cgtcgaagaa ttgtgtctga
    19801 gcgcgcagca aagcctctcg gcacgtcgag gcttcggtca ctgcatagcc ttcggactca
    19861 aggagatcat gcaggataat ctgtaagtcg ggatcgtcgt ctacgatcag aatctcaggc
    19921 tgctcaagcc gcttcttctc gacagaggac gttacagctg ctgccttctg catggccaag
    19981 acatcgtttc tttccctctc aggggtgagc aaaacatcga gagtggagat tactccttca
    20041 acggttccga aaccacgcgg gtctgcatgg agcggtcccc cgaagaagtt tctctgctgt
    20101 tgcgcggttg agcctggacc ttcatcgatt ggccaaccaa tcgaggccga ccggcacgca
    20161 gtggtttgga gcgtttggga aagggaccct gatgggcctg ttccagttca gccagggatg
    20221 tgcggacaga tcgtgcaacc atccaggcaa gcgtgagtcc cggagtccag tagaccgcgt
    20281 cgcgcatctg ctcaacgaga tccgatggca gattgacggt caatcgcacc attcgaggct
    20341 ttgcttcaat ggctttgatc accggcgtcc cgctgagctg cattgcgcgt cggagttttt
    20401 cctctgcggc agtggacgtt gcctgagaga tcatggtcat aggcacctcc ttcactatct
    20461 ggtgatatgg gctcggcttg ccatccattc gggagcgcac aggcccagga atgaagacgt
    20521 tcccggatta gctggaaggc cgcgtgccga ttaaggacag acacgcggcc aggatggcag
    20581 agatacgtgg cgacggcatc gctccagatg ggcatgatcc ggagggcatc ccacaattga
    20641 gacagaggct tccctgtcgt catgggggtc cgtcggcgcc gtaccttgcc aatggaagtc
    20701 caactcgtga tcgtcgccat ggtctggttc tcaatgaatt gctgcggtac cctaagcaac
    20761 ctctgtacca gcgtcatagc gcattgcgtc agaaggaagc agaagaggca agcgctcgaa
    20821 ctgtctagca tttaaaaact ttgtgttggt ttgccgtcga gacgggaaaa tcaacgccga
    20881 gaacagcagt atcggaaaca atactgctat tatctgcaga gatacgtcgg gaaataggct
    20941 acgaccggtg aagggaatga ggggatctga tgttctattg atgcagatgc ttcatcgcgg
    21001 cggcgcaaag aatgatgaag aaacccatcc agagagcggc acgctggaag ggaatcactt
    21061 cgtaagactc gccctcttct gtctcgtcat ctgatttgaa gatcactgtg tagaggaaca
    21121 gggttaagag gccgagcacg agggagagct tgatgaaaaa gaccgtgagg tacttggcgt
    21181 ggtgctgtac gccgctgccg gtttgagacg tgagcacctg attcacgtaa tggagattaa
    21241 tccctcccgt aaataacaag acaaccaaca gaatgcctgc cactttcttg tagtacttat
    21301 aaaaaatgtc cgtaatgggc ttaatctgct ccttcgcaac ggctttctgc aaaccgggag
    21361 tgacggccat gacgaggaag gccagcccgc cgatccagat caccgccgac aacagatgaa
    21421 accaatgatt caggatcggg atgagggtgt tgagatctgt gacaatgctc tgtaaggcat
    21481 ccatttgata taaccgttag gcgtgaaacg tgaggcgtga ggcgtgctga gtgcgtattg
    21541 agaaaggatt ttttaccact ccttacccct cacgcctacc ccctcacgtc aaaacttgaa
    21601 gaccggcgcg tcgttcccaa accgtttctt gcggagttct tccattagct caatggtcac
    21661 cgtcgagatt ccattctcac gggcgtgctt ttccacgccc ttcttgacca tcgcgcggag
    21721 gaaataggga atgcggctga gccgcagggc tgaggcctcg tcccaggaga cttcggtctc
    21781 ttcttggatg ttgaaggcaa cttttatttt ttcgccgccc tttggtgtat agagacacca
    21841 ctggtcttca tccagccaat ctccatggtc cacataggga cgggcgcggc agccgccgca
    21901 gatatcggtg tattcgcagt cgccgcattt acctttgagc tgaggatagc ggaatgagtt
    21961 gaaaatatcg gatttttccc agaggtcgac aaaactattc tggcggacat tgccggcaga
    22021 aagcggcatg tagggacaag gcgtcagttc gccattcggc gtcacgcggg catagttggt
    22081 gccggcgagg caccctccgc ccatgtagcc tgtggctttc gtgatcggag agttcgggtc
    22141 cttttcatag gccagccgct tgaaatgcgg cgcgcagcgg gcgcggacca gcatgccttt
    22201 gtaattgtcc tggcagttga cgaggtagcc gagcacctcc tcgtactgcg ccggtgtgat
    22261 gtcggtcagc tcttcgccac ggcctgtgca gaccatgaag aagacgttca agacacgcgc
    22321 gccgatgcca tgggaccatt ccacgacggc gggaagttcc tgataattca tcggctgggc
    22381 gctgaagtgg acttggaact ggaggccgtt gcgcttgctg gcttcgatgc cggccacagc
    22441 tgcttcccag gctccgggga cgccgcggaa ggagttatgt ttggccggat cgagtgaatc
    22501 gatgctgatg ccgaccccca tgacgccgat ctcgaccagg gttttggcca tctgatcgtc
    22561 gatcaacatg ccgttggtcc cgaagacgac catgaatccg agcttcaccg cgcggcgcgc
    22621 gatatccagg atatcgggtc gcaccaaggg ctcacctccg gtgatgacga gcaagcaacc
    22681 tttgttgacc tcagcgatct gatcgatgag ccggtagcat tcttcggtcg agagttcgtc
    22741 gtctcctccg gcggatttcg tcgttgcgtc gaggtagcag tggtcgcatt tgaggttgca
    22801 ccgtttggtg aggttcagcg cgacgagata gggcttgaaa tcgtcgacgg tccggccgtc
    22861 gtagggacct gcgccctgcg gggcaggcgt aaagaactgc gtgatctgtt gttgaaacga
    22921 tccgagcgct cctgagagcg aaggaccgtt gaagatggga agcgatttac ccatgaaaat
    22981 tgaggttgta agtggggtat cggggtaagg ggggtagagc accaggagga caggcggtat
    23041 cccgttatcc ctttagcccg ctatcccttc ttccccgtgc cggtaaggtt ggcgatcatg
    23101 tccttgaggt tgccggtctt catggcctct tccatgtagc ccttggcctg ctcgatccct
    23161 tcgttggcga cttccaacgt gatgagggtg gtgccgatct tgtccgcatg cttgatgatc
    23221 agcgccttcg tgcagtctcg catgaacccc tccggtgcac gatccagcct cgcttgggca
    23281 tcgatggtcc aggtataggg agaggcttcg gcctcgccgt tgccgcttcc attgccgttt
    23341 tcatgggtgc cgttgccgtt gacggctgcc aacctcgctt cggcttctgc ggatgttcca
    23401 gctcccttgt tcgcaaaaat cggctggtag tcttctgaag cagcctcttt gatcatgggg
    23461 gcggcatatt ccagcgtgat tgtggtcatc ccgagtttgc gtgccgtctt ttcgattttg
    23521 gctttggcgc ggcggcgttg gaacccggcc ggcacggcgc ggatcgcttc cttcgcgtcc
    23581 ttcgtccact gcatcggcac atcgtcgtag gcgacatcgg tttcgacccc gccttccgcc
    23641 ttcgcggctg cttcaaagat gctcttgtcg acctgtttga accgggcccc ttctgcaccg
    23701 cacaccgggc acttcaccgg cgtctcgccc ttgccgatgt atccgcagcc gtcgcagacg
    23761 aagtaattct gggtcatccg atccaggtag gcctgagtgc ctgcgctgac ggtcggcttc
    23821 tcttccacga tttcggtcat catgccgctg agcgtcgaac ccatgagatc cttcgccacg
    23881 gagtcgtccg cttgcatctt gtcccggtcg ataccggctg cctccagact gccgccgagc
    23941 gcgcgcatgg catccatggc gcctttgggc aggatatggc cgaccgccgc atccacgacc
    24001 gtattgctga tgatcgtatg gcccttctca atcgcataac ggtgaatggc cgtcttcgca
    24061 acaccgcggg cgaacacggg gatcttctcc atgcggcgca gggcctcttc cgtccaggcg
    24121 atcgtgtatt cggcttgcgt atcgatcggc ggcacgtact tgcggttgga gacgaggacg
    24181 ttacagggtg cactgcgaag cagattttcc gtgttgctgc cgatatccat atcctcgtcg
    24241 ctgtggacgc cgatccgacc gacgatcaga agggccggga catccttgcg gacgtactga
    24301 atgatctttt caaaggcttt gccgtcgagc aacgtggttt tcacgtccgt ttgttcggcc
    24361 tgagcgattt cacgggaaat gtccagatgc gattggtaga tcttggcgag gccgctgtcg
    24421 atgatctctt cgtgcagctt ttcctgctcc ttaaagcgga acaccttgcc cgcttcttcg
    24481 ttgaggacac cggaaatgct gtgaaacgcc gcgtaatgga agtacggatc gaacgccgaa
    24541 atggcttcca ccggcatgtt cagcgcctta ccgagtgaaa gaccggtcat gagtccgccg
    24601 aatgaatagg gactgccatc caccgcgacg acgatcttgc cgctggtgat cggctgggtg
    24661 ctcttgatga tgagcatatc cgagttgcgg acgcgacgaa cgacgcgctc cgtattgctg
    24721 ccaatcacgc tgtctttcac ggcgccgacg cccagcgctc ccatgatcac caggtcatag
    24781 gcgttggtat tgatatcttc agccaggacc ttccagtttc ggccctcgag cgaccgccgt
    24841 tcgaccggca ggttggcttc gttgcacttc ttgtcgacgt aatcgagata ggaatcggtg
    24901 atgatctgga gaccgcgggt aatcagcgaa tcatggatct ggcgttgccg gtcgagttcc
    24961 ttctcgtcgt ggtattcctc cggcaagccg gcttccatct gcttgaagcg cttgtcatgc
    25021 atcttggccg cgtagacgtg gcttccgacg atcttggaac cgaacatctt ggccaagtga
    25081 acgccgacat ccaccgccgt attggaatgg tcagaattat cgaccggaac gtaaatggtc
    25141 ttatacatcg agatgcctcc tccaggcgct ctcacagtca gggaagatcg tcataattgt
    25201 caaaaaaacc aaacggttga cgaacttcac ccctgtaaaa gaaggggcat gatagcaccg
    25261 gctactttga gaatgcaagg aggtcggcgg gctggtttgg aacgttcagc cgacggagct
    25321 ggtcagcggt ttttgctcgg aatccgttga tggtttgagc gattttctgg tccgtagtat
    25381 ctcctccaag gagagtaagg catcgcgccc tgagttccct tcgattccac gaatcaggtt
    25441 cgcatcggcg tacgtttctt tttctgtcgg ggtttgcgaa agcgacccgc tccactttcg
    25501 tggatcacgg ctcccgtttt tccgctccca ggcctttagg caagccttcg cgataatctt
    25561 gtttaagggc gcagggttgt agaggagctt cctgatgctt gtcggagtca gcatcaaggg
    25621 attgtaaccg gccgacaggt agccttcttc gagcaatcga tgctccaaat ccgtgtttgg
    25681 ctggataccg aggaagaaca tcagggggaa tacccgttcc tcccccagga tcgccgccac
    25741 cattttgtag gactccacgc tttggaggag actctcttcc gtttccttcg ggctgttaag
    25801 cgagtagttg aggataacct tccccttgaa tcccgcttcg gccagatagc ggcagccgtc
    25861 gtaaagccgt tccagcttga agcccatgtg cagattgttc aggacttcct gcgaacccga
    25921 agtgatggcc acttccagat cccctacacc tgatcgcacc atcagcttgg ccagttcggg
    25981 cgtgatcaag gaggtgcgga tatagccgga ccactcgatc tccatctttt cgctgatgat
    26041 tcgctccagg atctccgtac actggggata ggcttccttt cccgtgatga actgggcatc
    26101 cgtgaaccag aaccgtcttg tgccccattg gtgatagtgc tgggaaacgt cctttaccac
    26161 catctcgggc ggccgatagc gtacccgctt gccctcaatg taggggtaga ggcaaaaggc
    26221 acagtcgtac gggcagccac gcttggtctg cacaccgatt gcctcgccct ggaattcccg
    26281 gtactgcgga aatatcgagg tgagataggg gagatccact gtcagggcgt ccaggagggc
    26341 cggtgaaccc tgttgtccct ttctgatcct ctttccttct ttgacgatgt aacgctcatc
    26401 ctcgattgag cggccttcaa tcactttcag aatggcatct tccccttcac ccagcaggcc
    26461 gatcgtgccc tcgggcagtt tttcaatcaa ttgatcggca aaggcggtaa acgcgccccc
    26521 gccgatcata atctgggctt tggggaattc ctttcggatc agccagggat aggacaggat
    26581 cgtatagata tggctgtaat agcggtagag ctgcttcacc ccttcgaagg aagccacgat
    26641 gcgcttgagc ggattgctcg cgaaatagaa attgaaggca tgctcgagcg acgaatcgcc
    26701 ttcgtgcggg gagaagatct ggatgtcgcg ccaggagaag cagaccaggt ccggcttgaa
    26761 ttcggtggcg gtatcgcgga tcgcctgact gcgtttcgag acaggaaaca gcgacaaatc
    26821 aagaatgcgc tggcggacct ccggtttgcg gcggtgaatg aagtcggcca agtaggtgat
    26881 cccgatagga taaaccttct tgcaggggag aaagatgtag agaatggagt tcatgatttg
    26941 tcgttcgttg ttcgatgttc ggggttaaaa gtttgtgccc aaagcgccgg gtcgtcgaac
    27001 ttcgaacggt gaacttcgaa cctgttattt aaccgccaca tgtatcgcca cgatgccgcc
    27061 ggttagattt ttgtacgaca cttgttgaaa ccccgcctcg cggagcactt ggcagagccg
    27121 ttcctgatcg gggaatgtcc ggatcgatgc cggcaaatat tcgtagacgc cggtggtatc
    27181 tcgcgcgact ttcgtgccga tccacggcag tagcgtgaac gaataccaat catagagtgc
    27241 gcggagccag ccaaagacgg ggcgggaaaa ctccagacag acgaatcgcc cgcccggctt
    27301 catcacacgg cggatttcac tgacagcctg cggcaagttc cctacattgc gcatgcagaa
    27361 gccggtcgtc accgcatcga aaatgttatc ggcaaacccc agctgttcgg cattggcttg
    27421 caggcaagta atcttgccgg ttagcccttt gcgatcgacc ttctttagcc cttcccgcaa
    27481 catggcatgg ttgagatcgg atgcgaccac acgccccttg gctcccatgc gcggttcaac
    27541 caagagcgcg agatccgcag tgccggctcc gacgtcaagt gccgttcccc gctcgacgac
    27601 agggacaaag gaagcagtca attttttcca gcggtaatgg agaccgaaac tcagcagggt
    27661 gttgttgaga tcgtagcagc gggcgatcgc ggtaaacatc cgttggacgg cctgctcgcg
    27721 cgcctggccg gaccaggttg agacggcttg gggtggagtc ggtgaattct gtgccatagg
    27781 cggttgttcg gcccgtacca tggcagggtg gtagcacctg ctttctcgct ttgtcaaggc
    27841 aaggaggccg gatggtataa tccgccgcct atgggcaact caatcgtcat caagggtgcg
    27901 cgcgagcata atctcaagaa tatcgacgtt gagattccgc gcgac
//
LOCUS       DATIFR010000236         9435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2846504, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000236 DATIFR010000000
VERSION     DATIFR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846504"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VLD60_13560"
     CDS             <1..533
                     /locus_tag="VLD60_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSB46036.1"
                     /translation="TNEIHNAFCPSREVAMPLLKRLQSYLDTNKIPYEVVSHPKAYTA
                     HDVAQSLDVPGNLVAKVVVVKADGYFVMTVLPSTWRVDLKRLRDVLDVREVRLATEAE
                     IANLFPDCQVGAMPPFGNLYGVEVYVDQLLTEDESIVFEAGTYVGAMKLRYKDFAALV
                     RPKVAAFHHEPSKIER"
     gene            complement(652..1203)
                     /locus_tag="VLD60_13565"
     CDS             complement(652..1203)
                     /locus_tag="VLD60_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46037.1"
                     /translation="MKRTGAITTMAILAGLFMVSWCHHPTPSAAAVEIAPGTVAEVDQ
                     TVMKELVAAFDRAEVAVQHADLDALMGFYAKGYNYQGLKRPDVRRVWDEVFSHYGQVM
                     SKHVFTELKLVQAGGVKKAYVTCTGGLYGTEKETGKSVTIDSWVSEIHHLVKEKDGWQ
                     FLGNVGGASPAAPPSSAPHHPLF"
     gene            1461..2189
                     /locus_tag="VLD60_13570"
     CDS             1461..2189
                     /locus_tag="VLD60_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB46038.1"
                     /translation="MPTSLLDIHGLTFAVDRHPILDRLDLEIQAGEIHALLGANGSGK
                     TTLAYVLMGCDGYGPSTGSILFNGTDLLPLKMHERAQLGLTLAWQEPARFEGLTVREY
                     LSLGKSGCNPESALTQVGLDPDRYLTRLVDKALSGGERHRLELASVLAMKPKLAILDE
                     PAAGIDMLSINHIIDIIRALKEGGGSVLLITHQEEVALIADRASQLCAGRIIFSGGPR
                     EAVDHFRGRTCVRCDGEVCHYVRS"
     gene            2176..3111
                     /locus_tag="VLD60_13575"
     CDS             2176..3111
                     /locus_tag="VLD60_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HSB46039.1"
                     /translation="MSDLDELRRTLPMVGAEPAILDDLRIAHVVAQGHHILSHRTVPG
                     LRVDLEETRDAIIGKLIVEAGAQIAQPIHMCFGLAHPTGVQQIKIDVQVLEGAQARVL
                     SHCLFPFAKAAEHRMQAVMEIGPGASLTYTEGHYHGPHGGMQVLPHATIKIGKGARYF
                     SDFSLLSGSVGSLEIDYLVEVEEEGLAELSAKIFAHKTDHIILKEAVMLRGERSRGLI
                     KTRVVLEDRAKAEITGITEAYAKGARGHVDCMEIVQGEAHASAIPIVRVFHPEAKVTH
                     EAAIGSVDKKELETLMARGLAPEEAIELIVSGILR"
     gene            complement(3153..4301)
                     /gene="dnaJ"
                     /locus_tag="VLD60_13580"
     CDS             complement(3153..4301)
                     /gene="dnaJ"
                     /locus_tag="VLD60_13580"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSB46040.1"
                     /translation="MPGVDTKRDYYEVLGVDRAATRDQIKHAYRQLALKYHPDKNRDA
                     GAAAKFRELAEAYAVLSDDTKRKEYDTTGHAGVSERWSPDDLMRDFQFGDFFGGRFAD
                     LSGMFGDLFGRRTRPGAGAARGVDLRYDVELTLEEAARGGEREIHITRSEKCAPCGGS
                     GAKAGTKPLSCPDCGGTGQRQDVKTKKDMRLVTLTTCVRCQGRGQIVEFPCVLCQGSG
                     YQFVPHTLKVKIPPGVDDGMMIRLSGQGEANANGGPPGDLLIRPHLRRHPSFERHGDD
                     LYMVQSVTFPDAALGRKLRVAGLGGETLQVMIPAGTQGGTALRLSGKGMPKVGGKGKG
                     DLFVVVEVRTPTDLTERERALLQELAKLQKPPSPKDVPHNPKPDQQEG"
     gene            complement(4627..5184)
                     /locus_tag="VLD60_13585"
     CDS             complement(4627..5184)
                     /locus_tag="VLD60_13585"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSB46041.1"
                     /translation="MRRLTALMAVVLAAGCAGAPVVPPELKEKVDGTVTFAQLKASPL
                     SYKGKIIVAGGVVLSVKPLKQNGTRIEILQMPLESDYEPLGQLTESGGRFLAVHREFL
                     DPATIPIGTRVTVVGEVTGSMILPMDEIEYVYPTLEIASLTIWPSKLPAFWIRPYPYM
                     GAYWGSYWGPYWGPPPSMARPGERK"
     gene            5429..6358
                     /locus_tag="VLD60_13590"
     CDS             5429..6358
                     /locus_tag="VLD60_13590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB46042.1"
                     /translation="MIRMPNDSFRGPLPPLTEEQHGLEQDLRTHVQRLAGTIGERNVF
                     HYAQLVAAADYIRATLTSSGYEVRRHLYEISGQPCENVEAEIQGQKRPADIILIGAHY
                     DSVQGSPGANDNATGVAAMLALARVFAKVQASRTLRFVAFTNEEPPLFQSRHMGSRIY
                     AARSRERGEQIALMLSLETIGCYSDEPGSQAYPFPFRFFYPSTANFIAFVGNMDNAPW
                     VKQLLTAFRRHTQFPSEGAALWEWIPGVAWSDHWSFWKEGYPAVMITDTAPNRYPYYH
                     TGADTPDKVDFAKMARVVSGLQRVIFELAGESP"
     gene            complement(6645..7193)
                     /locus_tag="VLD60_13595"
     CDS             complement(6645..7193)
                     /locus_tag="VLD60_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46043.1"
                     /translation="MTSRLSLSALAILVGAFGLTLCGPPKPVTAKVEVPSYATVTADD
                     ATVKDLLAAFDRAEQAIQARDVDGLMALYSKRYNYHKLRKSDARRLWEEIFEYHHKLH
                     STHLFTDIKTVQVDGETRVEVKCTGALWGLANETGQKVTVDSWFEEVHYLVKEDGAWR
                     IIGNAGTGAETPRFGHAPHPLF"
     gene            complement(7217..7720)
                     /locus_tag="VLD60_13600"
     CDS             complement(7217..7720)
                     /locus_tag="VLD60_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSB46044.1"
                     /translation="MAKEKDKASKEVAVKKSAEVMPRGGEIDRWFDRLTEDFWRRPFP
                     SLLFGDRWPLPMISIKAPSLDVFEEKDELVVKADLPGMNKDEIEVTVTENVVTIKGEK
                     KKEEEVKEKDYYRRERSYGSFVRSVELPCEVKSDQIKANFKDGVLEVRMPKTEEAKKK
                     AVSIKID"
     gene            complement(7805..8545)
                     /locus_tag="VLD60_13605"
     CDS             complement(7805..8545)
                     /locus_tag="VLD60_13605"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB46045.1"
                     /translation="MKSADQSERDRVLKEVRAALEREPRINLHKCPVTMEFSDGVLTL
                     EGEAEHIAAKKLSLERAIAVRGVTGIVDRLHVAPSTHMGDGAVLDAVRDALLQEPTLT
                     NCSIYVIRKGQLETVREIAEEPHGEIRVSVTNGVVLLDDHVTGLTEKRLAGVLAWWVP
                     GSRDVVNGMEVVPEQPDSDEEMAKAVRIVLKKDPLVNGERVRVSAKNAVVTLEGAVPS
                     GPQKEMAEFDAWYVFGVDKVVNRLDIRP"
     gene            complement(8829..>9435)
                     /gene="amrS"
                     /locus_tag="VLD60_13610"
     CDS             complement(8829..>9435)
                     /gene="amrS"
                     /locus_tag="VLD60_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSB46046.1"
                     /translation="TAGYVCDEPREEFYRFMDAANVDLKGFTERFYKQICGGSLQPVL
                     DTLVYIKHHTNVWLEITTLIIPGENDSEAEIERLIQWVMEYLGPDVPIHFSAFHPDYK
                     MLDKPDTPPATLAMARTIAVKNGIRYAYTGNVHDEEGGSTYCHHCRKKLIGRDWYELR
                     EWNVTEDGACCFCRTPCAGVFESEPGRWGARRVPIILNQFR"
BASE COUNT         1950 a         2942 c         2510 g         2033 t
ORIGIN      
        1 aaacgaacga gatacacaac gccttttgtc catcgaggga ggttgccatg ccgctcctga
       61 aacggctcca aagttatctc gacacgaaca agatacccta tgaagtggtc agtcatccga
      121 aagcctacac cgcccatgac gtggcccagt cgcttgatgt gccgggtaac ctcgttgcga
      181 aggtcgtggt cgtaaaggcg gatgggtatt ttgtcatgac ggtccttccc tcgacgtggc
      241 gcgtggatct gaaacgattg cgggatgtcc tggatgttcg cgaagtgcga ctggcaacgg
      301 aagccgagat tgccaatctc ttccccgatt gtcaggtcgg cgcgatgccg ccgttcggca
      361 atctctatgg cgtggaagtg tatgtggacc agctcctgac ggaagacgag tccatcgtct
      421 tcgaagctgg aacatacgtg ggtgcgatga agctacgata taaggacttt gccgcgctcg
      481 tgcgcccgaa ggtcgccgcg tttcaccatg agccctcgaa gatcgaacgc tagccggctt
      541 tcagagactc aatgtcctga tgtgtacgac ttgctttttg gcttgttgag gcgtgccgga
      601 ctgactgttt gactatccta ccttgatcat cctagttatc ccgccaggtt atcagaacag
      661 tggatggtga ggcgcgcttg acggtggagc agcgggagag gctcctccca cattgccgag
      721 aaattgccag ccgtccttct ctttgacgag atgatggatc tcgctcaccc aactatcgat
      781 ggtgacggac tttcccgttt ccttttccgt tccgtacaat ccgccggtgc aggtcacata
      841 ggctttcttc accccgccgg cctggaccag tttcagttcg gtaaacacat gcttcgacat
      901 gacctgtccg tagtggctga agacctcgtc ccacacacgt ctgacatccg gtcgcttcag
      961 gccctgatag ttgtaccctt tcgcatagaa acccatcagc gcatcgaggt cggcatgctg
     1021 caccgcaacc tccgcccggt cgaatgccgc caccagttcc ttcatgaccg tttgatcgac
     1081 ctccgcgacg gtgcccggcg caatctcgac agccgccgct gagggagtcg ggtgatggca
     1141 ccaggacacc atgaagaggc ctgccaggat ggccatagtt gtgatcgcac cagtcctctt
     1201 catgctgtct gaaagacgca atcacgatgc catggccgcc atgtgcagaa cctcttgaag
     1261 atctcggcaa ttatttgctt ctttccttgc ctctcgcgac cggagagctc tgggatgacg
     1321 cctatctccc caccgcatgt tcctgctctg ccgcaaaacg ctacgctcgg gcctctctat
     1381 aatcatatcg aagcttagat cgatcgctca tgaccgggca tgcctgatgc agtaaaattc
     1441 acacggattg gtatttgata atgcccacta gcctcctcga cattcacggt ctcacgttcg
     1501 cagtcgatcg acacccgatt ctcgatcggc tcgacctgga aatccaagct ggagaaattc
     1561 atgccctgct cggagccaat ggatcaggga aaaccacgtt agcctatgtc ttgatgggct
     1621 gcgatggtta cgggccgagt acgggaagta ttctgttcaa tggcacagat cttctgccgt
     1681 tgaagatgca cgaacgcgct cagttgggcc tcaccctcgc gtggcaagaa ccggcccgct
     1741 tcgaaggcct caccgttcgc gagtacctca gcctgggcaa gtcagggtgc aatccggaat
     1801 cggcattgac acaggtaggc ctcgatccag accgttatct gactcggctg gtggataagg
     1861 ctctcagcgg cggagaacgc caccgccttg agttggcctc cgtgctggcg atgaagccga
     1921 agctggccat cctcgatgag ccggcggcag ggatcgacat gctctcgatc aatcacatca
     1981 tcgatatcat tcgcgccctg aaagaggggg gaggatcagt gttactgatc acccatcagg
     2041 aagaggtcgc gctcatcgcc gatcgcgcat cgcaactctg tgccgggcgc atcatttttt
     2101 ccggcggtcc acgggaagcc gtggatcatt tccgcggtcg gacctgcgtc cgttgcgatg
     2161 gagaggtatg tcactatgtc cgatcttgat gagctgagac ggaccctgcc gatggtcggc
     2221 gccgaaccgg ccattctcga cgacctgcgc atcgcccatg tcgtagctca aggacatcac
     2281 attctgagcc atcgaaccgt tcccggactc cgcgtggacc ttgaagaaac gcgggatgcc
     2341 atcatcggga aactgattgt tgaggcgggc gcgcagatcg ctcagccgat tcacatgtgc
     2401 ttcggcctcg cacaccccac cggcgtgcag cagatcaaga ttgatgtgca agtcctcgaa
     2461 ggggcacagg cgcgggtgct gtcccactgt ctctttccct tcgcaaaagc ggcagaacat
     2521 cggatgcagg ccgtgatgga aatcggacct ggcgcgtcat tgacctacac cgagggccac
     2581 taccacggtc cccacggcgg catgcaggtg ctccctcacg cgacgatcaa gatcggcaaa
     2641 ggcgcccgtt atttttcgga cttctccctg ctctccggtt cagtgggatc tctcgagatc
     2701 gactatctcg tcgaagtgga ggaagagggt ctcgccgaac tcagcgccaa gatcttcgcg
     2761 cacaagaccg accacattat cttgaaggaa gccgtgatgc tccgggggga gcgctctcgc
     2821 ggcctcatca agacccgcgt tgtgctggag gaccgggcga aagctgaaat caccggcatc
     2881 accgaggcct atgccaaagg tgctcgcggt catgtggatt gcatggagat cgtgcaaggc
     2941 gaggcccatg ccagcgccat ccctatcgtg cgggtctttc atccggaggc gaaagttacc
     3001 cacgaagccg cgattgggag cgtggacaag aaagaattgg agacgctgat ggcacgggga
     3061 ttggccccgg aggaagcaat tgagttgatc gtgagcggaa ttctccggtg atgtgatagt
     3121 ccctcaagag cgaaaaagcg cagcgtcttt cctcaaccct cctgctggtc aggttttgga
     3181 ttatggggca catctttcgg tgacggtggc ttctgtaact tcgccagttc ctgcagtagt
     3241 gctcgctcgc gttccgtcaa gtcagtcggt gtccgcacct ctaccaccac aaagagatcg
     3301 cccttccctt ttcctccaac tttaggcatc cctttcccgc tcaaccggag cgccgtgcca
     3361 ccttgggtgc cagccggaat catcacttgg agtgtctccc cgcccaaccc cgccacgcgc
     3421 aactttctgc ctagcgccgc atcagggaat gtcactgatt ggaccatata gagatcgtcg
     3481 ccatgacgct cgaagctggg atggcgccgg agatgcgggc ggatgaggag atcaccggga
     3541 ggaccgccgt tcgcgttcgc ctctccctgg ccggacagac gaatcatcat cccgtcatcg
     3601 actcccggcg gaatcttgac cttcaacgtg tgcggaacaa attgatagcc gctgccctga
     3661 cagaggacgc acgggaattc aacgatctgc ccgcggccct gacaacgcac gcaggtggtc
     3721 aaggtcacga gccgcatgtc tttcttcgtc ttcacgtctt gcctctgtcc ggttcctcca
     3781 caatcgggac aggacagtgg cttggtccca gccttggccc cactgccgcc acatggcgca
     3841 catttctcgg accgcgtgat gtgaatctcc cgctcgccac cgcgagcggc ctcctccagg
     3901 gtcagctcca cgtcgtatcg aaggtccacc cccctcgccg cccccgctcc cggcctcgtg
     3961 cgccggccga acagatcgcc gaacattccc gacagatcgg cgaagcggcc gccgaagaaa
     4021 tccccgaact ggaaatcccg catgaggtcg tccggtgacc atcgttcgct cacccctgcg
     4081 tggcccgtcg tatcgtattc ttttcgcttc gtgtcgtcag acaggacggc ataggcttcc
     4141 gcgagttcgc ggaatttcgc cgcagcgccg gcatccctat tcttgtcggg atgatatttc
     4201 agtgccaact ggcgataggc gtgcttgatc tggtcgcggg tggcagcccg atccaccccg
     4261 agcacttcat agtaatccct cttggtatcg acccccggca cttgcgactc ccccctacca
     4321 cgcctcttgg accaaatcgc gatcccctgc gacggcaaga ggcaactccg tcaacagaac
     4381 cgcaaccttc tgacactcct cgccattact tctgtgaatg cggaagacat gaagcgacta
     4441 gcggccgaac cgacccatga gttgcgactc tgccgggaca tgccccttgc acccctccaa
     4501 tacttgagcc ccgcggcaac gcgatgacaa tcgtgtcctt cgcccctttg taatggagaa
     4561 gtctctgagc cagctgtttg ccggcatcat gccgatcttt gaatagcgca aactcgccgc
     4621 gcggtatcat tttctctccc ctggccgtgc catcgaaggc ggaggacccc aataaggccc
     4681 ccaataggat ccccagtacg cacccatgta cggatagggg cggatccaga aagccggtag
     4741 cttggaaggc cagatcgtca acgaggcaat ctcgagtgtc ggatacacat actcgatctc
     4801 gtccattggc aatatcatgg atccggtcac ttcaccaacc accgtcactc gtgttccgat
     4861 aggaatcgtc gccggatcca ggaattctct gtggaccgcg aggaagcgcc ctcccgattc
     4921 cgtcaactga cccagcggtt cgtagtcgct ctcaagcggc atttggagaa tctcgatccg
     4981 cgtaccgttc tgtttgagcg gcttgaccga gagcacgacc cctccggcca cgatgatctt
     5041 tcctttatag gacagcggcg aagccttcaa ctgagcgaac gtgacagtcc catccacttt
     5101 ctctttcaac tcaggaggaa cgaccggagc gccagcacac cctgcggcca gcacaacagc
     5161 cataagagca gtgagacgtc tcatctcatt ctccaaattc ctgagaaggc ccttccacaa
     5221 tgtacagcaa gacgagtgcc caggccgaat atgtaaccgg taacggaagc tactgggtct
     5281 tcacaagaaa ttcaaatgag ccttcaccag tggagtcgtc cgatcgggaa gactgagggg
     5341 aatttccaca gttggcgatc agcgttgcgg cgtgaagggg cagtggcgct cttcgctgag
     5401 gagccacatt gtgcgagaat agctcaccat gatccgcatg ccgaatgaca gctttcgagg
     5461 gcctctgcca cctctcacgg aagaacagca tgggctcgag caagaccttc gtacgcacgt
     5521 gcagcggctc gcaggcacga tcggagaacg caacgtcttc cattatgcgc agctcgtcgc
     5581 ggcggcggac tatatccgtg cgacgcttac gagttccggc tatgaggtgc gccggcacct
     5641 ctatgagata tcaggacaac cgtgcgagaa cgtggaagcg gaaatccaag gccagaagcg
     5701 tcctgcggac atcattctca tcggcgccca ctacgactcg gtgcaaggca gtcctggggc
     5761 aaacgacaat gccaccggtg tcgcggcgat gctggcgctt gcacgggtct tcgccaaggt
     5821 acaggcatca cgcaccctgc ggttcgtcgc gtttacgaac gaagagccac cgctctttca
     5881 aagccgccac atgggaagcc gcatctatgc ggcacgaagc cgcgagcgag gcgaacagat
     5941 cgccttgatg ctaagcctgg aaacgatcgg ctgttactcc gatgaaccgg gaagccaagc
     6001 gtatccgttc ccgttcaggt tcttttatcc ctccactgca aacttcatcg ccttcgtcgg
     6061 caacatggac aatgcaccct gggttaagca gctgctcaca gcctttcggc gccacacgca
     6121 gtttccttcc gaaggagcgg ctctctggga atggattcct ggcgtcgcgt ggtccgacca
     6181 ctggtcattc tggaaggaag gctatcccgc cgtcatgatc accgataccg cgccaaatcg
     6241 ttacccctat taccatacag gagccgacac acccgacaag gtcgactttg ccaaaatggc
     6301 ccgcgtcgtc tcagggctcc agcgagtgat ttttgaattg gcaggcgagt ccccctgatt
     6361 attggctgcg tgatcgtgac aaatccctgt aagacaccag cggcatgttt cctgaacgca
     6421 ttgtcgggct aatcggattt acctttccac cagaaaatcc acagaagtac tgcaacaacg
     6481 gccccggcga gaatgaattc gaagacttca ccggacatag attcatccct tgaaacaggc
     6541 gccgctctca gtcaatttcg tcctgcttct cgacgaagtc ccccgtcacc cctaggtgat
     6601 acagagagac gagagtttcg gatcgatagc ttccatctct gccatcagaa cagcggatgg
     6661 ggcgcgtggc cgaaacgcgg ggtttcagcg ccggtccctg cattgccgat gatgcgccat
     6721 gccccgtctt ccttcacgag atagtgcacc tcctcaaacc agctgtccac cgtgaccttc
     6781 tgcccggttt cgttggccag accccagagc gccccggtgc attttacttc caccctggtt
     6841 tctccatcga cctgcaccgt cttgatgtcc gtgaagagat gggtggaatg cagcttgtgg
     6901 tggtattcaa agatctcctc ccagaggcgc cttgcatcgg acttacgaag cttgtgatag
     6961 ttgtatcgtt tcgagtagag ggccatcagc ccatccacgt ctcgcgcctg aatagcttgc
     7021 tccgcgcgat cgaacgcggc gaggaggtcc ttcaccgtgg cgtcgtcggc ggtgaccgtg
     7081 gcgtagctcg gcacctcgac cttcgcggta acgggcttcg gcggaccaca gagggtcagg
     7141 ccgaatgccc ccaccagaat agcgagcgca ctgagtgaga gccggcttgt catggatgcc
     7201 tccctgtccg aaacgcttaa tcgatcttga tcgagacggc tttcttcttg gcctcttcag
     7261 ttttcggcat ccggacttcc aggacaccat ctttgaagtt cgccttgatc tggtcgctct
     7321 tgacctcaca gggcagttcc acactccgaa cgaacgagcc gtacgaacgc tctcgccgat
     7381 aatagtcctt ctccttgacc tcctcttctt tcttcttctc gcctttgatg gtcaccacgt
     7441 tctccgtcac cgtcacttcg atctcatcct tgttcatacc cggcagatcg gccttgacca
     7501 caagctcgtc tttttcctcg aagacgtcca gcgacggggc ttttatcgag atcatgggca
     7561 gcggccaacg gtcgccgaac agaaggctcg ggaacggacg gcgccagaaa tcttctgtca
     7621 gccgatcgaa ccagcgatcg atctcgcccc cacgcggcat cacctcggca gatttcttga
     7681 ccgcgacttc cttgcttgcc ttatcctttt cctttgccat gacctctcct ccttgttagc
     7741 tgtgccgatt gttgctatag actagccgtt ctcgccgatt cccagttcca ccgttaccgt
     7801 tccgttatgg ccggatgtcc agccggttga cgaccttgtc cacgccgaac acataccagg
     7861 catcgaactc cgccatttcc ttctgaggtc cggacggtac ggcgccctcc agagtcacca
     7921 cagcattctt ggcgctgaca cgaacacgct cgccattcac gagagggtct ttcttcagga
     7981 caatacgcac cgcttttgcc atctcctcat ccgagtccgg ttgttccgga acgacctcca
     8041 tcccgttaac gacgtcgcga ctacccggca cccaccacgc caacaccccg gccaagcgct
     8101 tctccgtgag gcccgttaca tggtcgtcga gcaacaccac cccgttcgtc acggaaaccc
     8161 gaatctcccc gtggggctcc tcggcgatct ctctgacggt ctcaagctga cccttgcgga
     8221 tcacataaat tgaacaattc gtaagagtag gctcctggag cagcgcatcg cgtaccgcgt
     8281 ccaagacggc tccgtctccc atatgggtcg agggcgccac atgcagacga tcaacgatgc
     8341 ccgtcacacc tcgcaccgcg attgcacgct ccaagctcag tttcttcgcg gcgatgtgtt
     8401 cggcctcgcc ttccaatgtg aggaccccat cactaaactc catcgtgacg ggacacttgt
     8461 gcaaattgat gcgaggctcc cgctccaacg ccgcgcgaac ctctttcaga actcggtctc
     8521 gctctgactg gtctgctgat ttcatcgatt cacccagact cgaaaccccg acgtacctca
     8581 tccttgacgc aacgccaatg ccacgacagg tctttggggc gaagcccttg atgtttcagc
     8641 ggagacaggc cgaacgggtt cgcttgtgta agcgcggatc tgaggacatt ctccccaggc
     8701 ctcaacctgc tcatgacgaa aaacgctacg gagggagtaa gaatctaagg ggttacaggc
     8761 cgagaattga cgcactgttt tgcgacgcag aaagcttcag cgaggagatg caaattcgag
     8821 tgcggcgctt agcgaaactg gttcaaaatg atgggcactc gtctggctcc ccaacgcccc
     8881 ggctccgact caaaaacccc agcgcaaggg gtgcgacaga aacaacaggc gccatcctcc
     8941 gtcacattcc actctctcag ctcgtaccag tcccgaccga tgagtttctt gcgacagtgg
     9001 tgacaatagg tgctcccgcc ctcttcgtca tgaacgttgc cggtataggc ataccggatc
     9061 ccgttcttca cggcgattgt gcgggccatg gccagggtgg ccggtggcgt atccggcttg
     9121 tccagcatct tatagtccgg atggaacgcg ctgaaatgga tcggcacatc cggccccaga
     9181 tactccatca cccactggat cagccgttcg atctctgcct cggaatcgtt ttcacccggg
     9241 atgatcaaag tggtgatctc cagccacaca ttggtgtgat gtttgatata gaccagcgtg
     9301 tctagaaccg gctgcaggct tccgccacag atctgtttgt agaaacgctc ggtaaatccc
     9361 ttcagatcca cattcgccgc atccatgaat cgatagaatt cttcccgagg ctcatcgcag
     9421 acgtatcccg ccgtg
//
LOCUS       DATIFR010000237         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2463794, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000237 DATIFR010000000
VERSION     DATIFR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463794"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VLD60_13615"
     CDS             <1..201
                     /locus_tag="VLD60_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46047.1"
                     /translation="HRNSPAAVPLIKAPAHEWNGRGFTTNRIAIQGLARWVSISGLGL
                     RLPPKKERDISCVFWYTMVHGH"
     gene            191..2545
                     /locus_tag="VLD60_13620"
     CDS             191..2545
                     /locus_tag="VLD60_13620"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSB46048.1"
                     /translation="MDTKSTIQERLAGGDGTALVKEWLRDNKGKGRAALARHVCERLD
                     MRDARGRLRQGGAQKALRVLEARGYWRLPKRQSRGPGRWKPRRLGQRVAEPRKMPERV
                     EQVQGLSLVEVSREQDELFRTWNELIETEHPLHDSRMVGRQVRYLIGSEHGWLGALGF
                     GSCALRLAPRDEWIGWDEPTRRLFQDRVLDMRRFLIRPSVHCENLASRVLSMAAERVG
                     PDFARRYGFEPWLLESFVNTEAYEGTCYQAANWLCVGESSGRGRNGPNKPQVARKEVY
                     VYELNRRWRRQMGIVPKSEQITPLSLEESLCAEGWVEAEFGEADLGHQDTTQRLLRIA
                     KAKARNPSASYTECFAGDRHELKAYYRFIGKQRAQMSPEGILSGHRRQTIRRIKGYGR
                     VLAIQDTTDLDFSDRLHCNDLGDIGKNQTGAVSQGLKMHSALALSEDGLPLGVLGTHI
                     YASHFDSEDKAQNRPIEEKESYRWLRTIEELREMAQWVPETELIAVGDRESDLFELFD
                     YRRRKARNIHLLVRARYDRCLEEESRKLFAHLEALPVMGEAHIAVPRQREKKGKPSKP
                     GRIGLPARRAKVQLHWDKVTISAPQTTQTRHMAPVELHALLLREVDPPKGAKPLHWVL
                     LTTVQIESRKQALRCVRWYSRRWRIEEWHRVLKSGCRIEAHQHQSAAKLARAIAIDTV
                     MAWRVMLLTLLGREAPELRCELIFDPWECRLLEELQPFVAPETMRGQKKGLYASPPPI
                     SSSRDSAGPSTETPANFPDLKRSCAVSVASTTSKSATGWGKEYL"
BASE COUNT          583 a          745 c          949 g          525 t
ORIGIN      
        1 catcgtaatt ctccagcggc cgttccactc atcaaggcgc ccgctcatga atggaacggc
       61 cgcggtttta cgacgaaccg gatcgcaatc cagggcttgg cgcgctgggt atccatcagc
      121 gggctagggc tgcgcctgcc cccaaaaaaa gaacgggaca tttcttgcgt tttttggtac
      181 accatggtgc atggacacta aaagcacgat tcaggaacgg ttggcgggtg gggacgggac
      241 tgcactggtc aaggagtggc tcagagacaa caagggcaaa gggcgggcag cactggcgcg
      301 ccatgtgtgt gagcgtctgg atatgagaga tgctcggggt cgattgcggc aagggggcgc
      361 acagaaggcg ttgcgggtac tggaggcgcg aggctactgg cgattaccga agcgtcagag
      421 ccgcgggccg gggcgctgga agccgcgacg gctgggccag cgggttgctg aaccgcggaa
      481 gatgccggag cgggtggagc aggtgcaggg gttgtctctg gtggaagtca gccgtgagca
      541 ggacgaactg ttccgaacgt ggaacgagtt gatcgaaacg gaacatccgc tgcacgacag
      601 ccggatggtg gggcggcagg tgcgctactt gatcgggtcc gagcacggtt ggttgggtgc
      661 actggggttt ggttcctgtg cgctgcgact ggcgccgcgc gacgaatgga tcgggtggga
      721 tgagccgaca cggaggcttt ttcaggatcg ggtgctggac atgcggcgat ttctgattcg
      781 tcccagcgtg cactgcgaga acttggccag tcgggttttg agtatggccg cggaacgcgt
      841 ggggccggac tttgcgcgac gatatggctt cgagccttgg ctgctggaga gcttcgtgaa
      901 taccgaggcg tatgagggga cctgttatca ggcagccaac tggttgtgtg tgggcgagag
      961 cagcggccgt ggacgcaacg ggcccaacaa gccccaggtg gcccgcaaag aggtctatgt
     1021 gtacgaactg aatcggcgct ggcgccggca gatggggatc gtgccgaaga gcgaacaaat
     1081 cacccccctg agtctggagg agtcgttgtg tgctgagggg tgggtggagg cggagtttgg
     1141 ggaagcggac ttggggcatc aggacacgac gcagcggctg ctgcgtattg cgaaagcaaa
     1201 agcgcgcaac ccgtcggctt cgtatacgga gtgcttcgcc ggagatcgtc acgaattgaa
     1261 ggcgtactac cggtttatcg gcaagcagcg ggcgcagatg agtccggagg gcattcttag
     1321 cgggcatcgc cggcagacca tacgacggat caaagggtat ggccgcgtgc tggcgatcca
     1381 agacacgacc gatctggact tctcggatcg attgcactgc aacgatctgg gcgacatcgg
     1441 caagaaccag accggcgccg tcagccaagg gctgaagatg cactcggcgc tggccctgag
     1501 tgaggacggc ttgccattag gcgtactggg cacccacatc tatgcctcgc atttcgacag
     1561 cgaagacaag gcgcagaacc gccccatcga agaaaaggaa tcctaccggt ggttgcgcac
     1621 catcgaagag ctgcgggaga tggcacaatg ggtccctgag accgaactga tcgcggtggg
     1681 cgaccgtgag agcgatctgt tcgagctctt cgactatcgt cggcgtaagg cccgcaacat
     1741 tcatctgctc gtgcgtgcgc gttatgaccg ctgtctggaa gaggaatccc gaaagttgtt
     1801 cgctcatttg gaggcgttgc ccgtaatggg tgaagcgcac atcgcggtac cgcgccaacg
     1861 cgagaagaag ggtaaaccca gcaaacccgg aaggatcggc ctgccggcac gccgggcaaa
     1921 ggtccaactg cactgggaca aggtgacgat cagcgcgccg caaacgacac aaacccggca
     1981 catggcaccc gtggaactgc acgcgttgct gctgagggaa gtcgatcccc ccaagggtgc
     2041 caaaccgttg cactgggtgc tgttgaccac agtgcagatc gaatcgcgca agcaagcgct
     2101 ccgttgcgtg cggtggtaca gccgtcggtg gcgcattgaa gaatggcatc gtgttctgaa
     2161 aagtggttgc cgtattgaag cccatcagca tcaaagcgca gccaaactgg cccgcgccat
     2221 cgctatcgat acggtcatgg cttggcgagt catgctcctg accttgctcg gtcgtgaggc
     2281 ccccgagctg cgctgcgagc tcatcttcga cccatgggaa tgccgcctgc tcgaagaact
     2341 tcaaccgttc gtcgctcccg agacgatgcg ggggcaaaaa aaagggctct acgcatcgcc
     2401 accgccaata tcatcctctc gcgactcggc ggggccctca acagaaaccc ccgcgaattt
     2461 cccggacctc aaacgatcat gcgcggtctc tgtcgcttcc acgacatcga aatcggctac
     2521 cggctgggga aaggagtatc tgtagatgcg attgctatca gaaggtttta gggatacgcg
     2581 cagggctagg gcctacttgg cactcgtgat gaatccgtga cactcaagac ggaaagtcgt
     2641 cttcttcctg cgcggaaact cgcggtggcg ctgagttgat cctgagtggt gaggttgtcc
     2701 gggttgcttt gggagctttg ttggttccgg tgggataagc cctgttggct ggaggagtcc
     2761 aagtttcacc aagcgcgccg tgatggttca tcgccgaatc tt
//
LOCUS       DATIFR010000238        43076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1124898, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000238 DATIFR010000000
VERSION     DATIFR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 43076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43076
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124898"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VLD60_13625"
     CDS             <1..1001
                     /locus_tag="VLD60_13625"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HSB46049.1"
                     /translation="GPRGIPGSRETGGDEMTTVPLRLGISRCLLGDEVRFDGGHKRDN
                     FLTDVFGRYVEWVSVCPEVEAGLGTPREAMRLVGNSQHPRLVTIKSGIDHTNALETIT
                     KKRIEELSELDLSGYVFKKDSPSCGVERVRIYNEHGMPSRSGIGLFARGFLEQFPLIP
                     VEEEGRLCDAPLRENFIERVFCYRRYQDLLRGGVTRQAVVRFHTIHKYLLLAHSQQHY
                     ELLGRLVGRAEQYRQKELAVRYGELFMKALAVKATVRKHVNVLQHILGYFKERLKSQE
                     KVELLGVIDDYHRGLTPLIVPLTLIKHYVQLFNVDYILDQVYLNPHPKELMLRNHV"
     gene            1010..1519
                     /locus_tag="VLD60_13630"
     CDS             1010..1519
                     /locus_tag="VLD60_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HSB46050.1"
                     /translation="MAREKQGVVLVGHGGIPKDCPQDLVTKLKRLEAQRRAAKMPPSP
                     EELELDAKIRRWPRTAATDPYQSGLETVAAHVQSRLDGALFAIAYNEFCAPTLEESVE
                     ELIRKGATSIIVTTTMFTPGGSHSEIEIPEILDQLRPRHPGVTLRYAWPFDLQQVAQT
                     LAEQIRRFS"
     gene            1613..2194
                     /gene="pyrE"
                     /locus_tag="VLD60_13635"
     CDS             1613..2194
                     /gene="pyrE"
                     /locus_tag="VLD60_13635"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSB46051.1"
                     /translation="MVTVKEQLAQAFHETHSFKWDKEKGFTLASGQISPFYVDCRALM
                     AHPSARRFVGQLAHEALAAVSLDCLGGLEIGAISIATTISDYAYGANPKREWRTFVVR
                     KQAKDHGLGKVIEGAIKPGDRALIVDDVLTSGGSLLKAIEAARQAGLTVTHALVIVDR
                     KEQEGRAKVEKEGVKLMSLLTIEDLTKHQRRAS"
     gene            complement(2205..2513)
                     /locus_tag="VLD60_13640"
     CDS             complement(2205..2513)
                     /locus_tag="VLD60_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46052.1"
                     /translation="MKTWSSLGCALALLLAGCAEGAKLAQDNEDGGIVTYPFKGEQGA
                     MLSPYRTEALAMIREKCHGAYSIIREGEAKGRARVAGAVEGGQEIVRERRWGIDFRCK
                     "
     gene            2587..3012
                     /locus_tag="VLD60_13645"
     CDS             2587..3012
                     /locus_tag="VLD60_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSB46053.1"
                     /translation="MLKRTAIMFLSVLVLLVATMTAVSAHHSYLLTVQQLREGLTKSP
                     SMTQKGFVLIDVRSPEEHAQGFIPGTDFNIDFREMKARHREIGAQPSDHIVVYCQSGH
                     RSNIAAETLADLGYNHVYNVSGSMNAWQEAGYPVETARR"
     gene            complement(3030..3908)
                     /locus_tag="VLD60_13650"
     CDS             complement(3030..3908)
                     /locus_tag="VLD60_13650"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HSB46054.1"
                     /translation="MAQSHGLQRSSAFCATVLLGAFLFCSDGPARAAAPAIGWERLAE
                     GLAIGVWNPSSSCQDVPSLIALEIDPARYRFSVHHYRNEPVPDPLDIHEWQVRTGHDL
                     VFNAGLFRENFAYLGLLYGNGQPLGSKRHGSWLGLFVAEPTGSGGQAAGILDLAADSF
                     DEQRPPYREAAQSLMLLDRTGKIRVKQTGKQAQQTIVAEEENGHILVLKTTAVASLHA
                     IGQCLHDTYPGIRQAMAMDGGSSSDLSISPLLLRAAGQIAGAHPWMSLLNAGTTGHIG
                     LPAVIGISPRGHTTKP"
     gene            complement(3993..5615)
                     /locus_tag="VLD60_13655"
     CDS             complement(3993..5615)
                     /locus_tag="VLD60_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB46055.1"
                     /translation="MKTPLRLLHLEDDPTDTDLVSATLTEAGLACVAHRVDTRCTFLA
                     ALQEGDIDLILADYSIPGFDGMSALALARQQRPDVPFLFVSATIGEELAIDAMHQGAT
                     DYVLKQRLGRLVPSVLRALRERDDRNERKRAEEALVQSEKQFRQAQKMEAVGRLAGGI
                     AHDFNNLLTVIMGYSHVLSAELGRSHPLHGKIEETQKACDRAVMLVRQLLAFSRKQPV
                     EPKILSLCRIVGNLEGMLERLIGSDIHLAMTLDPADCHVRADQGQLEQVVMNLVVNAR
                     DAMVNGGTLSIDIAPVELTKSPIYHVEPLPPGSYVRLSVVDTGGGMDRETQAHIFEPF
                     FTTKEEGKGSGLGLSTVFGIVTQSGGGIDVTSRIGQGTRFDIFFPRLAADAHPAIPTQ
                     ESGQLHKGTETILLAEDDPSVRTLIHDELRKLGYKVVEAKNGIEACLLATQQAGTLDL
                     LLTDMVMPGMGGRELAQHLAALKPDLRVLFMSGYTDDVGILTGLGEGITAFLQKPFTP
                     AALAQTVRKLLDAPAETQQPELRRDAAKPAAT"
     gene            complement(5635..6090)
                     /locus_tag="VLD60_13660"
     CDS             complement(5635..6090)
                     /locus_tag="VLD60_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB46056.1"
                     /translation="MTTVKPIVLAEDNPRDAELALAAMEEHHIADKVVICHDGAEVLD
                     YLYCRGPYKSRESGNPVVVFLDLKMPKVDGIEVLRTIKNDANLKPIPVVMLTSSREER
                     DLAQSYALGANAYVVKPVEFQQFISAVKELGVFWGVINEPPPGGTKSAA"
     gene            complement(6090..7580)
                     /locus_tag="VLD60_13665"
     CDS             complement(6090..7580)
                     /locus_tag="VLD60_13665"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB46057.1"
                     /translation="MEIATQDRPAPPPASAPPVSEPVPKHTPPSILRVERLSTWLAKW
                     SIEQRVLAGFGLVFAGILVISVVSYRNTSVVVQNSRLDARSHELVQLLGSIEEALDAA
                     ESGHRRYLVTGDESYLKAHHAIVEQMPDYAHYLQALVGNDLERRERMTQLEQLIKEQL
                     DIEASAIKRRAEQGADAVRHLALPGVAKRELDNIHRLIADLETTEQRSTRARVFQSTS
                     STRNTIALLGLGALLQLVLLGSVYYLIHHDITARRRVAAELRRKGELLQAANKELEAF
                     SYSVSHDLRAPLRHIDGYAALLSKTAGQSLNEKAQRYLQTISDSAKQMGQLIDDLLVF
                     SRMGRQEMLRSTVNVNQLVKTVIHDLRLDLQDKPISWKIGSLPDVSGDPSMLRQVFTN
                     LISNAVKFTGTRPEPRIEIGAMQQVPGEVTLYVRDNGVGFDMQYANKLFGVFQRLHRN
                     DEFEGTGIGLANVRRIVHRHGGRTWAEGALDHGATFYFSLPLKRTT"
     gene            complement(7707..9209)
                     /locus_tag="VLD60_13670"
     CDS             complement(7707..9209)
                     /locus_tag="VLD60_13670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSB46058.1"
                     /translation="MTFSLTMSPSDLLLLLPEIILTCWLCLLLIVDFSFPRLPKEQLA
                     YLSIAGLVATLGCLAWFDFSGISGTLFANMFVVDRMALFFKIFIVAATILVVLASIEF
                     VHRFTFFRGEYYFLVVMSALGMMFMASANDLLSVFVTLEFSTFGFYVLVSYLREDVAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMKSAQATPGLVIGFLLIFGALG
                     FKIGAVPFHSWIPDTYHGSPTPVTAFLSIAPKGAAFAILLRMFFVALATFKPTWVLLL
                     VAASILSMTYGNIVAIAQKNVKRLLAYSGIAQIGNVLIGLAAGTKMGNDAILFYLLTY
                     LFANLGAFAIVIAVSQAIGSEEIDDYSGLGRRSPFLAFSMLIFLLSLAGVPPLAGFIG
                     KLYIFVAAIKEGLYTLITVGLINIVISMYYYLIVVKKMYIVEPHDTSPLTVSGPMKAV
                     IYVGLAGTLIIGIYPQPVADWVVAATMMFSDLAGPAAALPPVSPIIPSGG"
     gene            complement(9206..10912)
                     /locus_tag="VLD60_13675"
     CDS             complement(9206..10912)
                     /locus_tag="VLD60_13675"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB46059.1"
                     /translation="MSDYTLLFILFAPFIGAIALIFVSNRQALLVRGIAAGSAGLSLL
                     ASLYLFYAYDPVKGGFQFVQKYEWSRQLGISLHLGVDGIGTPLVLASSVLLFAGIFVS
                     WHIKDRTKEFYIWLLILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDPEGLKLRDSVGFIFNFGANSKEYAAMKLVLFLSAFAVVALMGILLIYKYSGLN
                     TFDILQLREHAKLMNIPVLGTTLDKIIWILIFFGFASIAPLWPLHSWSPVGHAAAPAA
                     TSMLHAGVLMKLGHFSIIRVAFEILPDTTRELMPIAAVLCMFSIIYGGFVAFYAKDTK
                     YVIGYSSSSHMGYVFLGMAALDYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRD
                     IPSLGGLSNRMPFIAGCFVIACMASIGMPGTVNFIAEVMIIVGSWNKYPLQVIVAVVG
                     IVITLAYLFRMMRGLFYGPMDQKYSHSHDAVAVVDRLPLLIMISVSIGFGLFPMHLYS
                     VVRSGVDPLVAKITQVVPVAQTGEGSGVRSEAMLPVRTLDVPLVANNTLPLTPHTLRG
                     SE"
     gene            complement(10917..12482)
                     /locus_tag="VLD60_13680"
     CDS             complement(10917..12482)
                     /locus_tag="VLD60_13680"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB46060.1"
                     /translation="MFEQLESTFPILTCLLFFPLAGAAVLWLFDDEDMVRTSALTISL
                     VELALAIFVLVRFVPDSAAMQFVEHVRWIPALGISYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTVRHQTKLYMMSLLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKYVLYTLLGSVFMLVGIALLDINYHSYATLHNLDMAYSFDLLELLAVPI
                     PLNQQILIFWLMFLGFAFKAPVFPFHTWLPDVLLEGPIGMAVVLAGLKLGTFGFMRFS
                     IPLLPDASKSQTVISVVMVLGLAAILYGAIMALIQPDFRRLLAYSSISHLGFVVIGLF
                     ALNYQGLQGSLLTMINLGFSTAGLFFIAGFLYNRQQTTQMAAFGGMARYTPVLATFFL
                     LIGLASIGLPGTNGFVGEFLILLGTFKANWLYGSIAVTGVIFGAAYFLWYYERAMLGP
                     VGKAVKDSIGDLHLRETIIALSLSVMILWIGLYPAPFLRMMNGSVQALVDRLNQGTVA
                     AIGTIPPGQGKVN"
     gene            complement(12492..14459)
                     /gene="nuoL"
                     /locus_tag="VLD60_13685"
     CDS             complement(12492..14459)
                     /gene="nuoL"
                     /locus_tag="VLD60_13685"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSB46061.1"
                     /translation="MTDLLIKLIPVFPLLAVIANGLFGRYLWSHEWAHRLAWGSVGAS
                     FICTIAVFFDVLRTGTPHEVIAYQWIFGGDLTINLAYLVDPLTCVMLLVITGVGFLIH
                     VYSVGYMHGEDGFTRFFTYMNLFMVSMLLLVMGNNYAVLFIGWEGVGLCSYLLIGYYY
                     DKISAAKAASKAFVVNRIGDAGFLLAIFLVFINFKSLDYTKVFAQVSQLSPEMATAIA
                     LCLLVGAVGKSAQIPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDLSS
                     TAMGIVGLIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGIGAYTAAIFHL
                     MTHAFFKALLFLSAGSVIHALSGEQDIRKMGGLSSKIPWTHKLFFIGTIAIAGIPPFA
                     GFWSKDEIMAHAFTHHQYLLYGMAAIGAFLTAFYMFRLTYLTFYGRSRMDHHTEEHVH
                     ESPMVMVGPLMALGVLSLAGGFIGFPPEHGLLHQFLAPVAGTGGEHEVGTGLVLTLMM
                     IATGIALFGWGLAHYLYSMNPTAPDRWAANARSVYNTLLNKYYVDELYDLVFVEPTKK
                     LGLWLDWFDRVVVDGIVRAVGQLAELGAAGSTWAEKHVIYGSLNVIGYGNHLLAKQWR
                     QLQSGMVHHYAAIIVAGLFLLAVVIQFIVQR"
     gene            complement(14461..14763)
                     /gene="nuoK"
                     /locus_tag="VLD60_13690"
     CDS             complement(14461..14763)
                     /gene="nuoK"
                     /locus_tag="VLD60_13690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSB46062.1"
                     /translation="MVPLSAYVAVSAVLFITGLLGVLIRRNFIIVLMAVEIMLNAANI
                     NLVAFSHYLESMAGQLVALFVIAIAAGEAAVGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            complement(14777..15301)
                     /locus_tag="VLD60_13695"
     CDS             complement(14777..15301)
                     /locus_tag="VLD60_13695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSB46063.1"
                     /translation="MIPVFFAYFALMSMAAAVLTVALKNPVHCGLALLALLLHVSGLF
                     ILLNAEFLWAVQVVVYAGAILVLYLFVLMLLNLKTDERYFHNTYLYFLVPTVLGSLYV
                     LALLFKSPYGGAKGDAPAAAVLKEGDTYAVGMKMFSDYLLQFEIVGVFLLGAIIGAIV
                     LAKTPKPIDDKREA"
     gene            complement(15310..15897)
                     /gene="nuoI"
                     /locus_tag="VLD60_13700"
     CDS             complement(15310..15897)
                     /gene="nuoI"
                     /locus_tag="VLD60_13700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSB46064.1"
                     /translation="MSVGTFTKNILQAALFYEIWDAMKVTFKHMFHKPITFQYPREQR
                     TIPDTHRGALGLLRYDDGRERCVGCDLCEAACPSRCIKVISSEDAERPLQRYASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEFSTHDKRTLLFDKRRLYDIGERHLDDAKKYL
                     YAHNQEKNVEESREYRYYFPQSVIKATQSAPKHLS"
     gene            complement(15917..18604)
                     /locus_tag="VLD60_13705"
     CDS             complement(15917..18604)
                     /locus_tag="VLD60_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB46065.1"
                     /translation="MGLKPATNPDVEAATIELNIDGKTVTAKDGVSLYDVISSTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKATAGMIIRTEGDDLFQARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLTNAKQKFIPEDTRSLVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDLPLSCEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKRTTTTCNYCGDGCQMYLETKDTEVIRVTSPLSWENKWGDRAETAKGH
                     GGLCVKGRFGFEYIDSPNRLKQPLLRKGNQLAEAPWLEAMHHVVDRFAEIRKKHGPDA
                     IAGLITARCTNEELYLFQKLMRAGFRTNNLDSSARYGHMNFVYASKEALGIGRTPNDW
                     EDLTKAKAVILIGSNITETNPLTAVRIKEAIRVFKAQVMVFDSAITNIGKLASHPFLI
                     KPGTEGLVIDGLVKAALELDLVDEESVAKHPQAFAALKSAVAGLSLEQIASRTGLKVE
                     NLKDAVTIFAEAPRSIILCAEGIVRQPNAYQNVLKLIDLAWITGKLGQPGCGVNTVTE
                     EPNEQGAVDMGAAPEFLPGLATFNDQATRDRLARAWDVTLPAVGAGANLMEILKHCRS
                     GQVRALYVVGENPLATLPASMEVRAALDRLDLLVVQDPFLTETARMAHVVLPACTYAE
                     KDGTITNLEGRVLRVRQAMDVIGESVPDWHIMTALANGLGCQWSYESANDIQAEIMKL
                     LPGYYNLGQPRKIVPTADTYLSNGYAAAVGRRYKTAAPASQQPFTLLMGQILYHSGKL
                     STAAPGLIKIAPNTGRLRMNPSDMERLGLQDGTTVNVTSEQGSLKLGVQQDQSVAPQT
                     CFFPEHFNEPPVKDLMTVQIDATTGVPSFKRTRVTIEKA"
     gene            complement(18687..19931)
                     /gene="nuoD"
                     /locus_tag="VLD60_13710"
     CDS             complement(18687..19931)
                     /gene="nuoD"
                     /locus_tag="VLD60_13710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSB46066.1"
                     /translation="MAFEDQRTTVYKVDPTHPESETLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLMNITVPERAEYLRTIVAEVQRIIGHQFWLGTQALDIGAMTVFFYCFRDREI
                     LLDWFDELCGARLTTSWYRIGGVERDFTPSLLAKLKQFLEYFPPKIDEYVVFLEKNRI
                     WLARTKGVAVISAEDAVSFGLSGPTLRGSGVDYDLRKAEPYSAYPKCEFNVPLGKNGD
                     TYDRYWIRVMEMYESVKIIKQCLEQMQDGPIMADVPSVTLPPKERVFTNLESMIQQFK
                     LFSQGFNAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSRG
                     YMIADAITLFGTYDIVMGECDR"
     gene            complement(19945..20448)
                     /locus_tag="VLD60_13715"
     CDS             complement(19945..20448)
                     /locus_tag="VLD60_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSB46067.1"
                     /translation="MSLQQLASRIQENFTEGFVKAVEWRGDLAVTVTRDKLHEVALFL
                     HDDSAMDFDYIVHVSSVDWPDDEERFEVVYEFYSIKKRHRIRLKTRVPESDCVVDSLT
                     DIWMGADFMEREVYDMMGIRFRHHPDLRRILMPDDYTEGYPLRKDFPLRGRGWRDTFE
                     FLDETAR"
     gene            complement(20459..20986)
                     /locus_tag="VLD60_13720"
     CDS             complement(20459..20986)
                     /locus_tag="VLD60_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSB46068.1"
                     /translation="MGLIQLGRQEKDGAPDVITTTLEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFVKQPEQVKTSLKV"
     gene            complement(20977..21369)
                     /gene="ndhC"
                     /locus_tag="VLD60_13725"
     CDS             complement(20977..21369)
                     /gene="ndhC"
                     /locus_tag="VLD60_13725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSB46069.1"
                     /translation="MSGLELLLEYLTRYFPILLFIFVALAFGVVTLLISYLVQPKYPE
                     PEKLSAYECGSEPFSDARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFNDINKVSVI
                     GLIEMLVFIALFLVAYVYAWRKGALEWD"
     gene            21927..23426
                     /locus_tag="VLD60_13730"
     CDS             21927..23426
                     /locus_tag="VLD60_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB46070.1"
                     /translation="MIIYASKFTGCLLCVILGLSGLQAAWAEDPESVRFGEAARSFAA
                     GSIQQSTPQVGVVNVITGDNQTVGNRMRLGQNDVFYLKLKNPGDAAVGDIYTVFKRAR
                     KVFHPGNGRYMGYLVIRLAIVQVVDVDKELTTVQAIRSYGSVAPGDPVMKFSLPTEGD
                     SSSAPVSDDVMGMVVEIQADMGMTLVAQHNFVYLDRGSEDGLKTGDRMDVVRTGGGLP
                     PRKVGEVRILSTEPRTAAAVVTKSTSRIMKGDRFRSRVQTGEPAPTPVSQPGRQSLNE
                     SPNQAQNAARETRYSLSDLIKQLRYDSGDARIKPEGYQVLDQLIEQLKAAPPEQLIRV
                     EGHADNMEIGPSLKSIYPTNWDLSKARAGGVLRYLVEKGGIDSARISSIGYGDSRPVV
                     SNATETGRQKNRRVDIVLYSPESAKAPSEQAMKPTEAVESGYGVAGAEPDNQNASAPA
                     TVNVAPADSAKVDNSEAVPASSVPDANPAVGDQSTPAKLMDQPIAPSEK"
     gene            23552..25882
                     /gene="priA"
                     /locus_tag="VLD60_13735"
     CDS             23552..25882
                     /gene="priA"
                     /locus_tag="VLD60_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSB46071.1"
                     /translation="MSVNHLASSDRESDPLFADVIVPRHLAGPFTYRVPIPLKTVLHV
                     GHLVLVPFGRSVIQGAVISLTGVPPPTLAPEQLKEIRTLATNGQATEVPPDLLQLAKR
                     VAESYVAPWGQCLRLVMPPSGVVGAAGGRFLLTQKGRDALAAKTIAMSDERILLQRLK
                     KRPLGIKEATLRKSKDRGEAALLESLVEQGWIQEIQEPAQPRNQLRSCIATQSDRDLY
                     GADIRPTKMDSRYPKDWEDRIVQAIERRRASQLLIQAPAPQRLALLREGIRRTVRIGR
                     MVLVIVGEAERAESLTTALSEHDSVVTACLHSGLSEYQKADVWRQIREQRIHVVVGTR
                     SAVFLPLSSLGLVWIERDEDPALKEPQEPRFHAREVAWFRAQEAEALLVVGSAHLSLE
                     TLAGVEEGSRLLRIPLQRDESPRIEVVDLRGQDRAMILSPLLIDATREALARRAGVLL
                     FLNRKGYAGALICRDCGQAPRCPACRVAIAYYRQKGSLLCPYCGMTASIPDLCPSCAG
                     PRLRLIGEGTERVEEDVKRLFPQAAVLRIDGETMHKPKQAAALWDRVQQRQWDILVGT
                     QLLLRDDVVPPVGVVGVVQADAGLNLPDFRAAERTYHLLADAVGLAHPSSSGGRVIIQ
                     SYLPSHHAIQAVAQQDETIFRAEELTHRTTLGYPPAVHLIVLHISGSQEKIVQEAALA
                     WVTRLRLPGVNGKAAQQASGEDKATEQAESLTILGPVPSPVPKLRGRYRRQILIKSRA
                     RDAAVRAVRRTVEELEQTYPSRLVKFDVDVDPLEMW"
     gene            complement(25886..26335)
                     /locus_tag="VLD60_13740"
     CDS             complement(25886..26335)
                     /locus_tag="VLD60_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46072.1"
                     /translation="MVATWPSRLNAGLGSLVVTVGAWLAWDQLSIPGAGLVMASVAAF
                     LLWRGHTTGLVWAWSTLLLGVECFAWPIITMVRIQSAAAQPSDEQMATILSAVLMGLF
                     SAVFWIAFSYGLFKRAGGEQSVSSPELPGKSSRQSPPTAPIHPRKKR"
     gene            complement(26440..26811)
                     /locus_tag="VLD60_13745"
     CDS             complement(26440..26811)
                     /locus_tag="VLD60_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB46073.1"
                     /translation="MPSVLVVDDEDQVRQLIRTTFEESGYQVLEASSGTAALEQYRLT
                     PTDLVVIDIIMPDQDGLESISTIRHESPKVKIIAMTGSNDMIGILGILDVAKMLGAHR
                     VLHKPFEMKDLLEAAKSILQA"
     gene            complement(27012..27668)
                     /locus_tag="VLD60_13750"
     CDS             complement(27012..27668)
                     /locus_tag="VLD60_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB46074.1"
                     /translation="MISGKPAPIRLLLVDDHEVVRVGLRTVLHNNQGITIVGEAGSKA
                     QAAKEAKRLYPDVILMDVRLPDGSGVEACREILADHPNSRVIFLTSYTDDDSVLAAVL
                     AGASGYVLKEIDSAALIRAIRTVASGRSILDPTVTQRALEWIKAVPTTDGPVRTSSLS
                     PQEEKVLALVAEGLTNKEIATSLKLSDKTVKNYLANMFQKLHISRRAQAAAFFAKRQA
                     "
     gene            complement(27838..27921)
                     /locus_tag="VLD60_13755"
     tRNA            complement(27838..27921)
                     /locus_tag="VLD60_13755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27886..27888),aa:Leu,seq:gag)
     gene            28073..28762
                     /locus_tag="VLD60_13760"
     CDS             28073..28762
                     /locus_tag="VLD60_13760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB46075.1"
                     /translation="MIPTKALALPCTETRVAAFFDVDNTLLPGEASEVRFFRTLWRRG
                     VVGWPELRESAWWLLRQVPPLSLQPLRERKVYLAGKPVQVIEPLGEEFCREELCPVVA
                     ADGLRRMEEHRQAGHVIILITGSLDFLIEPIASALRVDRCIAVTLEARHGLYTGQLSS
                     PLPYGEGKRRLIQQLASELDLDLRTCFAYGDSPGDREILECVGNPTVVNPIRGMARIA
                     KRKGWPIQQWR"
     gene            28817..29452
                     /gene="queE"
                     /locus_tag="VLD60_13765"
     CDS             28817..29452
                     /gene="queE"
                     /locus_tag="VLD60_13765"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HSB46076.1"
                     /translation="MRITEIFHSIQGESSYIGQPCVFVRLTGCPLRCTWCDTEYAFYG
                     GQECSVDEVLERVGSFGCRLVEITGGEPLAQPEAFALVTKLCDAGYTVLIETSGAMDI
                     AVVDQRAHVILDVKCPGSGMTDRMYWPNLGKLTMKDEAKFVLADRQDYDWAREIMSRY
                     QLTERCVVLFSPVFGRLEPRQLAEWVLADRLPVRFQLQIHKFIWAPDMRGV"
     gene            29535..31049
                     /locus_tag="VLD60_13770"
     CDS             29535..31049
                     /locus_tag="VLD60_13770"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSB46077.1"
                     /translation="MNIMQVDLKVGRAEGQSAEVLALTHYEGEGLNKQDAAVIDKLLG
                     GSVRELVQTKEFEGKANESLLFHTQGKVPARRLLLIGLGKKKDLTLDVIRQALGQAAK
                     RVRQAKVDSFTVAVPVVIPKGYSPVEVAQAMTEGAILGGYQFTTYRTTDDSVGKDVGQ
                     MTLLAQHKNQVRQLAEGIRRGVATAEATVFVRDLCNHPSNVMTPVRIANEAKSIAKSE
                     ALTLKILEQKEMEQLGMGALLGVARGSHEPPKFIILEYNGAKKKDERPVVLVGKTITF
                     DTGGISLKPAENMEHMKADMTGGAEVLASIRAAARLKLPLRLISILPVAENMPGGRAM
                     KPGDIVKTLSGKTVEVQNTDAEGRLILADALAYAQRYNPAALVDIATLTGACVVALGQ
                     FAIGMFGTDQKLKEQVRKSGLKAGERVWEMPLWDEYFEQLRSDVADMRNIGGRGGGMI
                     TAALFLSKFTGDCSWVHLDIASTDWSERERAYIPKGPTGIGTRLLIQYLIDRSL"
     gene            31051..32178
                     /gene="nagZ"
                     /locus_tag="VLD60_13775"
     CDS             31051..32178
                     /gene="nagZ"
                     /locus_tag="VLD60_13775"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB46078.1"
                     /translation="MARIELTSREKIGQLFMIGFMGTSVTPELVSFIKEYKPGGVILF
                     SRNLESVRQIVELTNDLQRCSPRSPLLISIDQEGGRVSRLPRGFTIFPPCELLGRCNS
                     SELAYAAAATIAKELKAVGVNMNMAPVLDVNSNPDNPVIGDRAFGSTPDVVSEMALVT
                     AAALQDNKVVACGKHFPGHGDTNADSHKELPVVEASRDRLEVIELPPFRRAVTAGIAT
                     MMTAHVLYKALDDQLPATLSPTVITNLLREDLQYDGVVLTDDLEMHAIVDHYGVGDAA
                     VRAILAGCDVLLICKEREREVSAFEAIEQAVASGTISMERLNQSVARIQRVKQRFLLP
                     YKPAVISDAKLVVGCRSHQALLQSMQQAKSRLEKVGAFSRL"
     gene            32212..32598
                     /locus_tag="VLD60_13780"
     CDS             32212..32598
                     /locus_tag="VLD60_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46079.1"
                     /translation="MGVKKGDLIDERKRHPHACGLVVKVMGGGEGFEKIICCDHDLTE
                     EDVVNEMGSHSGRRKGQLPPAVILDEKKLYPDSCGLRVMIMDGGAGFKEIRCCGHSLT
                     ASSMRELQYGKMRGSEPDVTGPAGTA"
     gene            32640..33095
                     /locus_tag="VLD60_13785"
     CDS             32640..33095
                     /locus_tag="VLD60_13785"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSB46080.1"
                     /translation="MKRWCGVMEWLDRLGYLTAGFSLLVLGMVIFAQAWYVFATKAGQ
                     AGLLPSGLKLLNDLLLVIILLELFRTVIRFLQTEILDLEPYLAVGIIACTRRVLTASA
                     ELSHLPSMTETQFYQYLMDVGLNVTVIMVLVVGVFLVRKRPAESPAVSP"
     gene            33199..33624
                     /locus_tag="VLD60_13790"
     CDS             33199..33624
                     /locus_tag="VLD60_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46081.1"
                     /translation="MANLLEYVGCVHIYLGPYRGNPIALYLKRSDTSCQIGPKVYPWN
                     DVVGTGETPNKAAADFEDKWKAKGLSADMYSGPSWEGGVKPEKPAPPKPPAAPKPATS
                     TAAGSAGQPAASPAPGSAPAQTTAAPTPGPPAKPTGESS"
     gene            complement(33636..35144)
                     /locus_tag="VLD60_13795"
     CDS             complement(33636..35144)
                     /locus_tag="VLD60_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB46082.1"
                     /translation="MERDALSPSAVAPPAIGGSSVISRLFRTKPIEQILSDADHPTHR
                     LKKTLTAWDLTCLGIGAIIGTGIFVLIGTAIVGDTHRPGAGPGIVLSFILSGLTCALA
                     ALCYAEMSAMIPVAGSAYTFSYATLGEMLAWLTGWNLILEYGVACVAVAIGWSGYFNN
                     LLTLAGLELPHWATHPPGGSDGGIANIPAAIIVLLVTVILVVGIKESARATGIVVVIK
                     LAVILFFIGIGSTSVNPDNWTPFMPQGFAGVGAAAAIVFFAYIGFDAVSTAAEEAKNP
                     KRDVPIGIIGSLTVCTILYIAVAAVLTGLIPSSQIDIHAPVAEALRLAGFKWGAAVIA
                     IGAVAGITSVLVVMMLGQIRVFFAMSRDRLLGPWLSLVHPRFGTPHRATILTGIAVAT
                     LAALIPIGEAADMTNIGTFFAFVLVCISVIILRYTKPDHPRPFRLPFMPIVPILGTLA
                     CLGLMWQLPQLTWMRFGIWTVIGIAVYLGYGLKHSRLAGQSPDASLAARPLR"
     gene            complement(35203..35949)
                     /locus_tag="VLD60_13800"
     CDS             complement(35203..35949)
                     /locus_tag="VLD60_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSB46083.1"
                     /translation="MKPLIGVTPDFNAGDRKEWGGREPTYFLRARYVRAIEELGGIPI
                     VLPLVADLSARRCLLGSIDGLLLTGSGPDLAPELYGERQRYKFRVMNQQRTTFELEMA
                     QLARAADLPVLGICGGMQAMNVAFGGSLYQDIPSQISKPLQHRQPSPATTLSHSVTVA
                     RGSLLRKVVRAATMRVNSSHHQSVKNVAPSLIASAVAPDGIVEAIESPTQRFFLGIQW
                     HPEFLFERYRLHRQLFEAFLRAARRRPSSR"
     gene            complement(35953..36399)
                     /locus_tag="VLD60_13805"
     CDS             complement(35953..36399)
                     /locus_tag="VLD60_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46084.1"
                     /translation="MNSYAFLSRTAATALFKLVALTIILTLGTVTMAPSAWSQDAAPA
                     STGDGSEGGSASSAGMQAAAGLSTLIYLPLKAAFAICGGIVGGLTYAFTAGNENAAKS
                     VWTTSMYGTYIITPDHLRGDKPIRFLGVPESEAPAPAPSPTMEPVR"
     gene            complement(36533..37000)
                     /locus_tag="VLD60_13810"
     CDS             complement(36533..37000)
                     /locus_tag="VLD60_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46085.1"
                     /translation="MSTDSSPESAQFRVTLFVGPQPVEGKPFTYSTVFNVKKRSWKGG
                     VQVEVEINQPQIDAIISAIGFPHWLSDILLTVPLEDRPSYRDRGQELFIQAICWCKLD
                     LLLQSGLTQDNQCLTADRFVEELRTSVTTRQEFITSYVATELDLLPNDQTLSP"
     gene            37084..38283
                     /locus_tag="VLD60_13815"
     CDS             37084..38283
                     /locus_tag="VLD60_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSB46086.1"
                     /translation="MTTIQPERVAAIQRTMREEGTIDGWLFYDFRGSDPLSYRVLLLD
                     PAMHVTRRWYYWIPSEGTPRKLLHRIEPHVLDGLPGETDLYVSWEQQRQLLAGLLNGS
                     HRIAMQYSPLNAVPYVSRVDAGTIELVRGCGVEVVSSADLIQTFEAVWTDRQLESHQY
                     AAAALRRIVDEAFGHVRDAVTKSRGLTEYGLQEFILKRIHEAGMVTSSAPITAVNEHS
                     ADPHYGPKATGSAEIRCDDLVLIDLWAKQAAAGSVYADITWTAYAGRQVPARHRQIFD
                     LVRQGRDAALNFVQAQVRAGHRPFGYEVDAACRDVIRAAGYGDQFLHRTGHSIGEEVH
                     GNGANIDGLETHDTRRLMPHTCFSIEPGIYLPGEFGIRSELDVFVSERDALVFGLPLQ
                     SDLSPLL"
     gene            38400..39215
                     /gene="tatA"
                     /locus_tag="VLD60_13820"
     CDS             38400..39215
                     /gene="tatA"
                     /locus_tag="VLD60_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSB46087.1"
                     /translation="MFGTMGISELIIILVIVLIIFGAGKLPQIGEGVGKALKGFKKEV
                     HDIPQPEPVQAEAVETVPPQVQAPIQQAQPAPPTPASPAAAAPKPTAPYTPGPELTPG
                     TTAALMYKIAAQPQAPRPVAAQGAAPVLAADQGQRPPTMEERAAAPSPVTRAQYPPLP
                     PSAQQKPQVKRPSAIVNKDAVARVQAQQAALKAKASQPGGGVSPADMQSLGEGLGDAL
                     RTFKQAVADVRNSVDPEMRTIQAEMDAAQKEIQQSIEAARELPAVQEETQKPA"
     gene            39365..40033
                     /locus_tag="VLD60_13825"
     CDS             39365..40033
                     /locus_tag="VLD60_13825"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB46088.1"
                     /translation="MALLTPLLRDENADIRRTAAESLGKIGDQAAVIFILPLVSDPAP
                     AVRAAAVQALGRIALPATQEVVDALTQALQDPSDSVKEAASMAIGDIEPASNQLGLAA
                     SLVSAPDTQVRRATVRALRQVDASLWLSVLVSALRDSDAEVRQSTVAALGESGGMSMA
                     IEIRKRLTEDSSPAVRAEAAYQLGKFGGRDVKLALEQTVAKDPESGVRRWAEGELKSL
                     RVSD"
     gene            complement(40008..40526)
                     /locus_tag="VLD60_13830"
     CDS             complement(40008..40526)
                     /locus_tag="VLD60_13830"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB46089.1"
                     /translation="MKFSVVTTQGHEGKTLEIDTNHEVITLHGSDRTLLGAVSWKEII
                     ERIITSDEDTRFAHARAHPRAPLAVKVKYTTPEGKQFDSLTGGIGGGGFFIESSQPLA
                     PGTELGIEFSLPDRPWEKHKAKAKVAWTRNKPERYLLFPGMGVQFTDIDDKSRKELVE
                     LVAALNRSRVMT"
     gene            complement(40581..41102)
                     /locus_tag="VLD60_13835"
     CDS             complement(40581..41102)
                     /locus_tag="VLD60_13835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HSB46090.1"
                     /translation="MKYPVVSSLRHKGKTIELDSAREIVTLIGINGEQLGNLSWDFVI
                     DQILAYRKPPASREARTEPRVTLSFRVKYKTPEGQQFESRAGGIGGGGLFIESQAPLP
                     VGTKLTMEFSLPERPDEWLPAKGVVAWVCPKADQYTFSPGMGVRFTDIAQNIRDRVLE
                     LVKTLQQVGQPAA"
     gene            complement(41335..42591)
                     /gene="clpX"
                     /locus_tag="VLD60_13840"
     CDS             complement(41335..42591)
                     /gene="clpX"
                     /locus_tag="VLD60_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSB46091.1"
                     /translation="MAKQEKMDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPSEIKHHLDQYVVGQERAKKILSVAVHNHYKRISAKEKD
                     VDDVELQKGNILMVGPTGTGKTLLAQTLAKYLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQASDYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFVCGGAFVGLEQIIEQRLNRKSMGFGAEVRGRSD
                     VRLGELFEKVQPEDFLKYGLIPEFVGRLPVVATLDELDERALIRILTEPRNALTKQYE
                     KLLSFEKVKLKFTEGALAAVARKAFIQKTGARGLRAILEEVMLDVMYDAPSQKQIKEV
                     LITEDAILGKHPPIRIFEQDKDVRTA"
     gene            complement(42605..>43076)
                     /locus_tag="VLD60_13845"
     CDS             complement(42605..>43076)
                     /locus_tag="VLD60_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSB46092.1"
                     /translation="KDINLYVNSPGGSVTAGLGIYDTMQYVKPPINTICLGQAASMGA
                     LLLTAGTKGKRFALPNARVMIHQPLGGFQGQATEIDIHAKEILKIRERLNEIMAKHTG
                     QPIEKISHDTERDYFMSGEEAKRYGLIDEVITRPPKLLKAVGGSESGKDGAKDK"
BASE COUNT         9383 a        11837 c        12896 g         8960 t
ORIGIN      
        1 caggcccgcg aggaatacct ggatctcggg aaacaggcgg tgacgagatg acgacggtac
       61 cgctccgtct gggaatcagt cgctgtctcc tcggagacga agtccggttt gatgggggac
      121 ataagcggga caattttttg acagatgtct ttggacgcta tgtggagtgg gtctctgtct
      181 gtccggaagt cgaagcagga ctaggcactc cgcgtgaggc gatgcggctc gttggcaatt
      241 cgcagcatcc caggctcgtg acgatcaaga gcgggatcga ccacaccaac gcgttggaga
      301 cgataaccaa gaagcgaatc gaggaactca gcgagctgga cctatccggc tacgtcttca
      361 agaaggattc cccgagttgc ggcgtggaac gtgtgcgcat ttacaacgaa catgggatgc
      421 ccagccgaag cggaatcggg ttgttcgcca ggggcttcct cgagcagttc ccgttgattc
      481 ctgtcgagga agaaggaagg ctctgcgatg cgccgctcag ggagaatttt atcgaacgcg
      541 tgttttgtta ccgccgatat caggatcttc tcaggggtgg cgtgacgagg caggctgtgg
      601 tgcggttcca cacgattcac aagtatctgc tcctggccca tagccagcag cactatgaat
      661 tgctcggtcg gctggtcgga cgggctgagc aataccgcca gaaggaactc gccgttcgat
      721 acggggagtt gtttatgaag gcgttagccg tgaaggcaac ggtgcgcaag cacgtgaatg
      781 tgttgcaaca tattctgggc tacttcaaag agcggctgaa gagccaagag aaagtggagc
      841 tgttaggcgt gatcgacgat tatcatcggg gacttacgcc cctaattgtc cctctcacgc
      901 tgatcaaaca ttatgtccag ctcttcaatg tcgactacat tcttgaccag gtctatctca
      961 atccgcatcc gaaggagctg atgcttcgga accacgtgta ggaggtttca tggcgagaga
     1021 gaagcaaggg gtggtactgg tggggcacgg agggattccg aaggattgtc cccaggatct
     1081 tgtcacgaaa ctgaagcgct tggaggccca gcgccgggct gcaaagatgc ctccctcacc
     1141 tgaggaactc gagctggatg ccaagattcg acggtggccc aggactgcgg cgactgatcc
     1201 ctatcaatcc ggactcgaaa ccgtcgccgc tcatgtgcaa tccaggttgg acggggccct
     1261 tttcgccatt gcgtacaatg aattctgcgc gccgacattg gaagagtcgg tagaagagct
     1321 gatcaggaag ggcgcgacgt ccatcattgt gaccacgacg atgtttacgc cgggcggctc
     1381 gcactcggaa attgagattc ccgaaatcct cgatcagttg aggcctcggc accccggagt
     1441 cacattgcgc tatgcctggc cgttcgatct ccagcaagtg gcccagacat tggcagagca
     1501 gatccggcgc ttctcctgaa cgcggtcatc gtctaccccg tgaaaactgc tgttctcggc
     1561 tcggcgagcg gtgtacaatg ccgtcgtttt ttcatcagga ggcaggactt gcatggtgac
     1621 agtcaaagag cagttggctc aggcctttca cgagacacac tcattcaagt gggataagga
     1681 gaaggggttc acgctcgcat ccggccagat cagccccttt tatgtcgatt gccgggcact
     1741 catggcccat ccctccgcgc gacggtttgt cgggcaactg gcccacgaag cattggccgc
     1801 tgtctcgctg gactgccttg gagggcttga gatcggcgcg atttcgatcg cgacgacgat
     1861 ttctgactat gcttacggag cgaatccgaa acgagagtgg cggacattcg tggttcgcaa
     1921 acaagcgaag gatcatggat tgggaaaagt gattgaagga gcgatcaagc ccggtgatcg
     1981 ggcgttgatt gtcgatgatg tgctgacgag cggcgggtcg ctgctcaaag ccatcgaggc
     2041 agccaggcaa gcggggttga cggttaccca cgcgttggtc atcgtggatc gaaaagagca
     2101 agagggacga gccaaggtcg agaaagaagg ggtcaaactg atgagtttgc tcaccatcga
     2161 ggatctgacc aagcaccaga gacgagcatc ctaagacgtt cgagctactt gcagcgaaaa
     2221 tcgattcccc agcgccgctc ccgcacgatt tcctgcccgc cttcgaccgc accggcgaca
     2281 cgcgcccgtc ctttcgcctc cccttcccgg atgattgaat aggcgccgtg gcacttctct
     2341 ctgatcatcg ccagggcttc cgtgcgataa ggtgagagca tcgcgccctg ttcgcctttg
     2401 aaggggtagg tcacgatccc gccgtcttcg ttgtcttgtg cgagttttgc gccctccgcg
     2461 cagccggcca agaggagggc cagcgcacag ccgaggcttg accaggtctt catacggctt
     2521 ttatcataat gcagagttca ttctctgtcg aacatcctag gcgatgatgt gatcaggagg
     2581 cctgccatgt tgaaacgaac ggccatcatg ttcctcagcg tgctcgtgct gctggtcgcc
     2641 acgatgaccg cggtttctgc ccatcattcg tatctgctga cggtccagca actgcgggaa
     2701 gggttgacca aatccccttc gatgacgcag aagggtttcg tattgattga tgtgcgctcc
     2761 cccgaagagc atgcacaagg cttcatcccg ggaaccgact tcaacatcga tttccgcgag
     2821 atgaaggcgc gccatcgtga gatcggtgcg caaccgagcg atcacattgt cgtctattgt
     2881 cagtccggcc atcggagtaa catcgcggcg gagaccttgg ccgatttggg ttacaaccac
     2941 gtgtacaatg tcagcggcag catgaacgcc tggcaggagg ccggatatcc ggtcgagacc
     3001 gcgcgtcgct aatagcccac ttcccgtcac tatggtttgg tggtgtgacc gcgggggctg
     3061 atcccgatca cggcgggcag tccaatgtga ccggttgtgc ccgcattcag cagcgacatc
     3121 caggggtggg ctcccgcgat ttgcccggct gcccgcagca ggagagggct gattgagagg
     3181 tcggacgacg atcctccatc catggccatg gcttggcgga tacccggata ggtgtcgtgc
     3241 aggcattgcc cgatggcatg gagcgacgcc accgccgtgg ttttcagcac caggatgtgc
     3301 ccgttctcct cttccgccac gatcgtttgc tgtgcctgtt ttcccgtctg cttgacgcgg
     3361 attttgccgg tccgatcgag gagcatgagt gattgggcgg cctctcgata gggaggccgc
     3421 tgttcgtcga aggaatctgc agccaggtcc aagatccctg ccgcctggcc tccggaccct
     3481 gttggctctg cgacaaagag ccccaaccag gagccatgcc gtttgcttcc gagcggctgc
     3541 ccgtttccgt acagcaaccc cagatacgca aagttttcgc gaaacaggcc ggcattgaag
     3601 accagatcgt gaccggtccg cacttgccac tcgtggatgt cgagtggatc cggaaccggt
     3661 tcattccgat agtgatgcac ggaaaatcga tagcgggcag gatcgatctc cagggcgatt
     3721 aaggacggta cgtcttggca ggaggaggaa ggattccaga ccccgatggc gagcccttct
     3781 gccagccgct cccatccaat ggcgggagcg gcagcgcggg ccggtccgtc cgagcagaat
     3841 aaaaacgccc cgaggaggac cgttgcacag aacgcagatg accgctggag gccgtgagac
     3901 tgtgccatgg tgacgtgcgg ttgagtatag agaggcggga tgcgtagcgt caaattgatc
     3961 ggcgccggct caccgtcatg ttctgtacgg gatcaagttg cggccggctt ggcggcatcg
     4021 cggcgaagtt ccggttgttg ggtttcggcg ggtgcatcga gaagttttcg gacggtctgg
     4081 gccaaagcag caggcgtaaa gggtttttgc aagaacgcgg tgatgccttc tccaagcccc
     4141 gtcaaaatac cgacgtcgtc ggtgtacccc gacatgaaga gaacccggag gtccggtttg
     4201 agcgcagcga gatgctgcgc cagctccctg ccacccatgc cgggcatgac catgtctgtt
     4261 aggagcaagt ccaacgttcc tgcctgctgc gtagccagga gacaagcctc gatgccgttc
     4321 ttggcctcga ccaccttgta cccgagcttt cgcagttcat cgtgaatcaa tgtgcgcacg
     4381 cttgggtcgt cctccgcgag cagaatcgtc tcggttcctt tgtgcaactg cccggattcc
     4441 tgggtcggta tggcgggatg cgcatcagcg gcgagacggg gaaagaagat atcgaacctg
     4501 gtgccttgtc caattcggct ggtaacgtca atgccaccgc ctgattgcgt cacgatccca
     4561 aacaccgtcg agagaccaag gccgctcccc ttgccttctt ccttggtggt gaaaaacggt
     4621 tcgaagatat gggcttgagt ctcgcggtcc attccgccgc ccgtgtccac aaccgacagt
     4681 ctgacgtaag aaccgggcgg aaggggctcg acatgataga tggggctctt ggtgagttcg
     4741 accggagcga tgtcgatcga aagcgtcccg ccgttgacca tggcatcccg cgcattgacg
     4801 acgagattca tcacgacctg ttccagctgt ccttggtcgg cgcgcacatg acagtcggcc
     4861 gggtcgagtg tcattgccaa gtggatgtcg gatccgatca gtcgctccag catgccttca
     4921 agattaccga cgatgcggca caggctgaga attttcggtt cgactggttg cttgcgactg
     4981 aacgccagca gctggcgtac cagcatcact gcacgatcac aggccttctg cgtttcctcg
     5041 atcttgccgt gcagtggatg actgcggccc agttcggcgg aaaggacgtg actatagccc
     5101 atgatcacgg tcagcaaatt attaaagtca tgagcaatgc ctccggccag ccgtccgacc
     5161 gcttccattt tctgagcttg gcggaactgt ttttcgctct ggaccagcgc ttcctccgcg
     5221 cgtttccgct cgttgcggtc gtctcgctcg cgcaacgcac gcagcactga gggcaccagt
     5281 cggccaagtc tctgtttgag cacgtaatcc gtggcgcctt ggtgcatcgc gtcgatcgcc
     5341 aactcctctc cgatggtggc tgagacgaag aggaagggca cgtccggcct ctgttggcgc
     5401 gccagcgcca gcgcgctcat gccgtcgaat ccgggaatcg aatagtcggc cagaatcaga
     5461 tcaatgtcgc cttcctgcaa tgcggccagg aacgtgcaac gcgtgtccac ccgatgggcg
     5521 acacaggcaa gccctgcctc cgtcaatgtg gcggagacga ggtcggtgtc ggtgggatcg
     5581 tcctcgagat ggagcaggcg gagcggggtt ttcactgatc ctccgcaggg cggactaggc
     5641 ggcggacttt gttccaccgg gcggtggttc gttgatcacg ccccagaata ctccgagctc
     5701 tttgacggcc gaaatgaact gctgaaactc gacaggtttg acgacatagg cgttggcgcc
     5761 caatgcgtaa ctctgagcca ggtctcgttc ttctcgagaa gacgtcaaca tgacgaccgg
     5821 gatcggtttt agatttgcgt cgttcttgat cgttctcaat acctcaatcc cgtcgacttt
     5881 cggcatcttg aggtccagaa acacgacaac ggggttgccg gattctcgac ttttatacgg
     5941 cccccggcag tagaggtaat ccagcacttc agcgccatca tggcagatga cgactttgtc
     6001 tgcaatgtga tgctcttcca tggcggcgag tgccaattca gcatcacgag ggttatcttc
     6061 cgccagcaca atgggtttta cggtagtcat caggtagtcc tcttaagcgg gagtgaaaaa
     6121 taaaacgtgg cgccgtggtc gagggcgcct tcggcccagg tccggccgcc gtggcggtga
     6181 acaatcctcc gcacattcgc caggccgatg ccggtgcctt cgaactcgtc gttgcgatga
     6241 aggcgctgaa agaccccgaa gagtttgttg gcgtactgca tatcgaatcc gacgccattg
     6301 tcgcggacat agagcgtgac ctcgccggga acctgctgca tcgctccgat ttcgatgcgc
     6361 ggctcaggac gagtgccagt gaacttgacg gcgttggaga tcaggttcgt aaacacctgc
     6421 cgcaacatgg atggatctcc cgagacatcc ggcaatgatc caattttcca agagatcggc
     6481 ttgtcttgca agtcaagacg taaatcgtga atgacggtct tgacgagttg gttgacgttg
     6541 acggtgctac gcaacatttc ctgacggccc atgcgggaga ataccaacag atcgtcgatg
     6601 agctgcccca tctgtttggc ggagtccgaa atggtctgga ggtatcgctg ggccttttcg
     6661 ttcaatgact ggccggctgt tttcgacaac agcgcggcgt agccgtcgat atggcgcaga
     6721 ggcgcacgga ggtcgtgcga cacggaataa ctgaaggctt ccaattcttt attggcagcc
     6781 tggagaagct ctccctttcg ccgcaactct gccgccacgc gccgacgggc agtgatgtcg
     6841 tggtggatca ggtaatagac cgagcctaat agcacaagct gtaagagggc gcccagtccg
     6901 aggagcgcga tcgtattgcg ggtacttgaa gtggactgga agactctggc tcgagtggat
     6961 cgttgctccg tagtctcaag gtcggcgata aggcgatgga tattgtcgag ctcccgtttt
     7021 gccacaccgg gaagggcgag gtgacgaacg gcatcagcgc cctgttctgc gcgtcgcttg
     7081 atcgcgctgg cttcgatgtc taattgctct ttgatgagtt gttccagctg tgtcatccgc
     7141 tcccgtcgct cgaggtcgtt cccgacgagg gcttggaggt aatgcgcgta gtcgggcatt
     7201 tgttccacaa tggcatggtg ggctttgaga taggattcat cgcccgtgac gagatagcgt
     7261 cgatggccac tctctgccgc atcgagcgct tcctcgatcg atcccaagag ttggacgagt
     7321 tcatgacttc tggcatccaa tctgctgttc tgaaccacaa ccgatgtgtt gcgataggag
     7381 acgacactga tgacgagaat gccggcgaat accaggccga acccggcgag gacccgctgc
     7441 tcaatcgacc atttggccag ccaggtggag agcctctcta cccgcaaaat cgacggtgga
     7501 gtgtgctttg ggaccggttc agaaacaggc ggagcagagg ctggcggcgg cgcgggacgg
     7561 tcctgagtgg ctatttccat gcggttcaag cgcagagcga gctgaagggt tcagtgaaga
     7621 cttaagaaac tctactctgg ccgattgggc cctgcaagca atttttcaga agagcgtggg
     7681 actcgacagg attggagcga cgtcgcttag ccccctgacg ggatgatggg agacacgggc
     7741 ggaagcgctg cggccgggcc ggccaggtcg gagaacatca tggtggcggc gacgacccaa
     7801 tcagccactg gctgaggata gatccctata atcagggtgc cggccagccc tacatagatg
     7861 accgccttca tcggaccgga caccgtcaat ggcgaagtgt cgtgcggttc gacgatgtac
     7921 atcttcttga cgacaatgag gtagtaatac atcgaaatga cgatgttgat aaggcccacc
     7981 gtaatcaagg tgtacagtcc ctctttgatg gcggcgacaa aaatatagag tttgccgatg
     8041 aagccggcca agggaggcac gccagccaaa gacagtaaga aaatcagcat ggagaatgcc
     8101 aagaacggtg agcggcggcc caacccgcta tagtcgtcga tttcttcgct gccgatggcc
     8161 tggctgacgg cgatcacgat cgcgaaggct ccaaggttcg caaagaggta ggtcaggaga
     8221 taaaacagaa tggcgtcgtt ccccatcttt gtcccggccg cgaggccgat aaggacattg
     8281 ccgatctgag cgatgccgga ataggccagg agtcgcttga cgttcttctg ggcgatggcc
     8341 acgatgttgc cgtacgtcat cgacaagatc gaggcggcga cgagaaggag cacccaggtc
     8401 ggtttgaacg tcgccagcgc gacgaagaac atgcgaagca gaatggcgaa ggcggcgcct
     8461 tttggcgcga tcgacaggaa agccgttacc ggtgtcgggg aaccatggta cgtgtcgggg
     8521 atccaggagt ggaacggcac cgcgccgatt ttgaatccca aggcaccgaa gatcagtagg
     8581 aatccgatga cgagtcccgg tgtggcctgg gccgatttca tgtcggagaa gaccagcttg
     8641 ccggtttcac cgtagacaag actgatcccg taagccagca ggccagccgc aaagacaccc
     8701 aggatgaaaa atttgagacc ggcttcattg gatgccacat cttcgcgcag ataggaaacg
     8761 aggacgtaga aaccgaaggt ggaaaactcc agcgtgacga atacggacaa gagatcattg
     8821 gccgaagcca tgaacatcat gccgagggcg gacatcacca cgaggaaata atattctccg
     8881 cggaagaacg tgaatcggtg gacgaattca atcgaagcaa ggacaaccaa gattgtggcg
     8941 gccacgatga atatcttgaa aaacagcgcc atgcgatcga ccacgaacat gttggcgaag
     9001 agtgtcccgg agatgcccga gaaatcaaac caggcaaggc atccgagcgt ggcgacaagc
     9061 ccggcgatgc tcagataagc aagctgttcc ttgggcagcc gcgggaatga gaaatccacg
     9121 atcaggagga ggcagagcca acaggtcaaa atgatctcgg gtagcaacaa caggagatca
     9181 gacggcgaca tggtcagcga aaaggtcatt ccgatcctcg taaggtatga ggcgtaaggg
     9241 gtaaggtgtt gttcgcgacc agcgggacgt caagcgtcct cacgggaagc atcgcctcac
     9301 tcctcacgcc tgacccttca cccgtttgcg cgaccggaac cacttgagta atcttggcca
     9361 cgagcggatc gacgccggaa cgcacgacgc tgtagagatg catcgggaaa aggccaaagc
     9421 cgatgctcac cgaaatcatg atcagcaggg ggagacgatc cacgactgcg acggcatcat
     9481 gggagtggct gtatttctgg tccatcgggc cgtagaagag gccgcgcatc atgcggaata
     9541 aataggcaag agtaatgacg atgccaacga cggcgacgat cacctgaagc ggatacttat
     9601 tccaactccc cacgatgatc atgacctcag caatgaaatt caccgtgccg ggcatcccga
     9661 tggaggccat acaggcgatg acgaagcaac cggcgatgaa cggcattcga ttggagagcc
     9721 cgccgagcga cggaatgtca cgagtgtgcg tttggtcgta gacccagccg gccatggcga
     9781 agagcatgcc ggtggccatg gcatgggcga acatgtagat cacggctccg cttaagctga
     9841 tgtaatccag ggcggccatg ccgaggaaga cgtagcccat gtggctggag ctggaatagc
     9901 cgatcacata cttggtgtct ttggcgtaga aggccacgaa cccgccataa ataatgctga
     9961 acatgcagag cacagctgcg atcggcatca gctcgcgcgt ggtgtcgggc aggatttcaa
    10021 aggccacacg gataattgaa aaatgcccca gcttcatcaa gacgcccgcg tggagcatgc
    10081 tcgtggcggc cggcgcggcg gcatggccga ccggtgacca ggagtgcagc ggccagagcg
    10141 gcgcaatgga ggcaaaaccg aagaagatca aaatccagat gatcttgtcc agcgtggtac
    10201 ccagcacggg aatgttcatc agtttcgcat gctcgcgcaa ttgaaggatg tcgaaagtgt
    10261 tgaggccgga gtacttgtag atcagcagaa tgcccatcaa ggcgacgacg gcgaaggctg
    10321 agaggaacag gaccaacttc atcgccgcgt attctttgct gttcgcaccg aagttaaaga
    10381 tgaagccgac ggagtctctg agtttgaggc cttcgggatc ggtcatctcc agatattttt
    10441 tggtatggct accccacatg ccgagcagga ggtacatcgg gatcacggac atttcataga
    10501 agaagtacag gaagaacaga tccagcgaca tgaagacgcc gattgtggcg gcggcgagaa
    10561 tcagcagcca aatgtagaat tctttcgtcc gatccttgat gtgccaggag acgaagatcc
    10621 cggcaaacaa aagaacggac gacgcaagca caaggggggt gccgatgccg tcgactccaa
    10681 gatgaagcga aatgcccaac tgtcttgacc attcgtattt ttggacgaac tggaatccac
    10741 ccttgaccgg atcataggcg taaaagagat agagcgacgc gagcagcgac aggccggcgg
    10801 atccggccgc aatgcccctg accagcagcg cctgccggtt cgatacgaag atcagggcga
    10861 tggcgccgat gaagggagca aacagaatga ataacagtgt gtaatcgctc atgagatcag
    10921 ttcaccttgc cttgccctgg cgggatcgtg ccaatggccg ccaccgtccc ctgattcagc
    10981 cgatcgacga gagcctgcac cgatccgttc atcatgcgga ggaacggggc gggatagagg
    11041 ccgatccaga gaatcatgac cgagagggac agcgcgatga tggtttcacg gaggtgcaaa
    11101 tcgccgatgg aatccttcac ggccttgccg accggtccga gcatcgcgcg ctcgtagtac
    11161 cacaggaaat acgccgcacc gaagatgacg ccggtcacgg cgattgagcc gtacagccag
    11221 ttggctttga aggttcccaa gagaatgaga aactcgccga cgaaaccgtt ggtcccaggc
    11281 aatccgatag atgccagtcc gatcaggagg aagaaggtcg cgagtaccgg ggtatatctg
    11341 gccattcccc cgaaggccgc catctgcgtt gtttgttgtc tgttatagag aaagccggcg
    11401 atgaagaaga ggccggccgt gctgaacccc agattgatca tcgtgagcaa actgccttgg
    11461 agaccctgat agttcagcgc aaacagcccg atgacgacga accccaagtg gctgatgctg
    11521 ctataagcga ggagccgacg aaaatccggc tggattaagg ccataatcgc gccatagaga
    11581 atggcagcga ggcccagaac catcacgaca gaaatgacag tctggctttt cgaggcgtcg
    11641 ggcagcagcg gaatgctgaa acgcataaaa ccgaaagtgc cgagcttcaa tcctgccagc
    11701 acgacagcca tgccgatcgg cccttcgagc aggacatcgg gcaaccacgt gtgaaatgga
    11761 aagactggtg ctttgaaggc gaagccgagg aacatgagcc agaaaatcag gatctgttga
    11821 ttgagcggga tcggcaccgc gagcagctcc aaaaggtcga aggagtatgc catgtccaag
    11881 ttatggagcg tcgcgtagct gtgatagtta atgtccagga gcgcgatccc caccagcatg
    11941 aacacgctgc ccagcagcgt gtacagtaca tacttgaggg ccgcgtaatg gcgttccgct
    12001 ccgccgcccc acagcttgat gaggaagtag ctggggatca gcatgagttc ccagaagacg
    12061 aagaagagga tcaggtccag cgaaacgaac acgcccattg tcgtcgtttc gagcgccagc
    12121 agtgacatca tgtacagctt cgtctggtga cggacggtat cccacgagta aatcaccacc
    12181 aagacggtca ggaaggccgt gagtcctaca aaaaggacac tgatgccgtc aacggccagg
    12241 tggtagctga tcccaagagc gggaatccag cggacatgct caacaaactg cattgcggca
    12301 gagtccggta cgaatcgcac aagcacgaag atcgccaagg ccaattcgac cagcgagatg
    12361 gtcaaggcag aggtccggac catatcctcg tcgtcaaaca gccataggac cgccgctccg
    12421 gcaagcggga aaaagagcag gcacgtcagg atgggaaatg tggattccag ttgttcgaac
    12481 atggtgccgt tctaccgctg cacgatgaat tgaataacga ccgccagcaa aaataagccc
    12541 gcgacaatga tcgctgcata atgatggacc atcccgctct ggagttgccg ccactgcttg
    12601 gcgaggagat ggttcccata gccgatgacg ttcagactgc cataaatgac atgcttctcg
    12661 gcccaggtgg atcccgccgc gccgagttcc gccaactggc cgaccgcacg tacgataccg
    12721 tccaccacga cccggtcgaa ccaatcgagc cagagcccca acttctttgt cggctcgaca
    12781 aagacgaggt cgtagagttc atcgacatag tatttattca ggagcgtgtt gtagaccgat
    12841 cgagcgttgg ccgcccagcg atccggggcc gtcggattca tactgtagag gtaatgagcc
    12901 agtccccacc cgaacagcgc gatgccggtg gcgatcatca tgagagtcag gacgagcccg
    12961 gtgcctacct cgtgttcccc gcctgtcccg gcgaccggcg cgaggaattg atggagcaag
    13021 ccatgctcgg ggggaaatcc gataaacccc cccgctaagg agagtacacc caaggccatc
    13081 agcgggccga ccatgaccat cggagattca tgcacgtgct cttccgtgtg atggtccatg
    13141 cgcgacctgc cgtagaacgt cagataggtc agtcggaaca tgtagaacgc ggtcagaaac
    13201 gcgccgatcg cggccatgcc gtacaacaga tactggtgat gcgtgaaggc atgggccatg
    13261 atttcgtctt tactccagaa tccggcgaag ggcggaatcc cggcgatggc gatcgtcccg
    13321 atgaagaaga gtttatgggt ccagggaatt ttcgaactga gcccgcccat ctttctgatg
    13381 tcttgttcac cggagagcgc atggatcacc gagccggccg agaggaacaa caacgctttg
    13441 aaaaacgcat gggtcatgag gtgaaagatc gcggcggtgt aggcgccgat gccgcaaccc
    13501 aggaacatgt atccaagttg gctcacggtc gaataggcca ggacgcgctt gatgtccgtt
    13561 tgcacgaggc cgatcgtggc ggcgaacagc gcggtgagtc cgccgatcag gccgacaatg
    13621 cccatcgccg tcgaagacag atcgaaaatg gcatgattgc gcacgatcat gtagacgccg
    13681 gcggtcacca tggtggccgc atggatcaag gcgctgacgg gagtcggacc ctccatcgcg
    13741 tcaggcagcc aggtgtagag cggaatctgc gccgacttac cgacagcccc gacgagcagg
    13801 cagagggcaa tcgccgtggc catctcgggc gagagctgac tgacctgtgc gaacactttt
    13861 gtgtaatcga gtgacttgaa attgataaag acgaggaaga tggccaacaa gaatcccgcg
    13921 tcgccgatgc gattcacgac gaaggctttc gaggccgcct tggccgccga gattttgtcg
    13981 tagtagtaac cgatcaagag ataggaacag agccccacgc cttcccaccc gatgaagaga
    14041 accgcataat tgttccccat caccagcagc agcatggaga ccatgaagag gttcatgtac
    14101 gtgaagaacc gcgtgaagcc gtcttctcca tgcatgtatc caacggaata gacatgaatg
    14161 aggaatccga cgcccgtgat gacgaggagc atcacgcagg tcaacggatc aacgagatag
    14221 gccaggttga tggtgagatc cccgccgaag atccactgat aggcgatcac ctcgtggggc
    14281 gtgccggtcc tcagaacgtc gaagaagacc gcgatcgtgc aaataaacga ggcacctacc
    14341 gatccccagg cgagtcggtg agcccattca tgggaccaca ggtagcgccc gaacaggccg
    14401 ttcgcgatca ccgctagcag ggggaatacc ggaataagtt taatcagaag atcggtcacg
    14461 ctaccacttc aataagttca tttcgtcgac attcgtggaa atttttcctc ggaagacgac
    14521 gatgataatg gccaatccca cggcagcttc tccggcggcg atggcgatca cgaagagcgc
    14581 gaccagctgg cccgccatag actccagata gtgggaaaaa gcgacaaggt tgatgttcgc
    14641 cgcgttgagc atgatctcga ccgccatcag gacgatgatg aaattccgtc tgatgagcac
    14701 gccaagcaat cccgtaataa acaggacggc gctgaccgca acatatgcgc ttaacggaac
    14761 catcacttct tacccctcac gcctctcgct tgtcgtcaat cggcttcggc gtcttcgcca
    14821 gtacgattgc gccgatgatc gcgcccaaca ggaagacccc gacaatctca aattgcagga
    14881 ggtaatcgct gaacattttc attccgaccg cgtaggtatc gccttccttg agcacggccg
    14941 ccgccggcgc atcgcccttt gcccctccat agggcgactt gaacagaaga gccaggacat
    15001 acagcgaacc cagcacggtc ggcaccagga aatagagata tgtgttgtga aagtaacgct
    15061 catcggtttt gagattgagc agcatgagga cgaagagata caggaccaga atcgcgccgg
    15121 cgtagaccac gacttgaacg gcccagagga actccgcgtt gagaaggatg aacaggccgg
    15181 agacgtgaag cagcagggcg agcagcgcca agccgcaatg cacggggttc ttcagggcca
    15241 cggtcagcac cgcggcggcc atactcatca gggcaaaata ggcaaaaaag accggaatca
    15301 tcgtacggtt cagctcaaat gcttcggcgc agactgcgtc gccttgatga cagattgcgg
    15361 gaagtagtac cggtattccc gactttcctc gacgtttttt tcctggttat gggcgtacaa
    15421 gtatttcttg gcgtcgtcga ggtggcgctc gccgatgtca tagagccgcc gcttatcgaa
    15481 gagcaatgtg cgtttgtcgt gcgtggaaaa ctcatacatc ttcgtcatgg ccagcgcatt
    15541 cacggggcag gcctcgacgc aatagccgca gaagacacat ttcgtaatgt cgatatagaa
    15601 ctcgctggcg tatcgctgca gcggtcgttc cgcatcctct gaactgatca ccttgatgca
    15661 gcgggacggg caggcggctt cgcagaggtc gcatcccacg caacgttcgc ggccgtcatc
    15721 ataccgcaga agaccgagcg cgccacggtg ggtgtccggg atcgtccgct gctcgcgggg
    15781 atactgaaag gtgatcggtt tgtggaacat atgcttgaat gtcaccttca tggcgtccca
    15841 gatttcatag aacagggcgg cctggagaat gttcttggtg aatgttccga cactcatgtg
    15901 cgcctcccgt ctctccctag gccttttcaa tggtcaccct ggttcgtttg aacgagggga
    15961 caccggtcgt ggcatcgatc tgcacggtca taaggtcttt gacgggaggc tcgttgaagt
    16021 gctctgggaa gaagcaggtt tgaggcgcca cactctgatc ctgctggacg ccgagcttga
    16081 gcgaaccctg ctcggatgtg acgttgactg ttgtaccgtc ttgcaagccg agccgttcca
    16141 tgtcggacgg gttcattcgc agccggccgg tgttcggggc gattttgatg agacccggtg
    16201 cggcggtcga cagcttgccc gagtgataga ggatctgtcc catgagcaag gtgaagggtt
    16261 gctggctagc cggtgcggca gtcttgtagc gccttcccac ggcggcggcg tagccgttcg
    16321 acaagtacgt atcggctgtg gggacgatct ttctcggttg gccgaggtta tagtatccgg
    16381 gtaacagttt catgatctcg gcttggatgt cattggcgga ctcgtagctc cattgacagc
    16441 cgaggccgtt ggcgagagcc gtcatgatat gccaatcggg aacgctttcg ccgattacat
    16501 ccatggcttg ccggacgcgc aacacgcggc cttcgaggtt ggtgatggtg ccgtcttttt
    16561 cagcataggt acaggccggc aacaccacat gggccatccg agcggtttcc gtcaggaacg
    16621 gatcctggac gacaaggaga tccaaccgat ctagggctgc gcggacttcc atcgatgccg
    16681 gcagagtggc gagcggattt tctccgacga catagagggc gcggacctgc ccgcttcggc
    16741 agtgcttgag aatctccatt agatttgcgc cggctcccac ggccggaagc gtcacgtccc
    16801 aggccctggc caagcgatcg cgcgtcgctt gatcgttgaa ggtagccagg ccgggaagaa
    16861 attccggcgc cgcgcccata tcgacggcgc cctgctcgtt cggctcttcc gtaacggtgt
    16921 tgacgccgca gccaggttgt cccagctttc ccgtgatcca ggccaggtcg atcaacttca
    16981 gaacattctg ataggcgttt ggctggcgca cgatcccttc ggcgcacaga atgatcgaac
    17041 gaggcgcttc ggcaaatatc gtcacggcgt ctttgaggtt ctcgaccttg aggccggtcc
    17101 tggacgcgat ctgttccagc gacagacctg ccacggcgga tttcaacgcc gcgaaagctt
    17161 gcgggtgttt ggccacgctc tcttcgtcga ccagatccag ctccaatgca gccttcacga
    17221 gaccgtcgat gaccagtcct tccgtccccg gcttgatgag gaacggatgc gaggccagct
    17281 tgccgatgtt cgtgatcgca gaatcgaaca ccatcacctg ggccttgaag acgcgaatag
    17341 cttccttgat gcggaccgcc gtcagcggat tggtttccgt gatattggaa ccgatcagga
    17401 tgacggcttt ggccttcgtc agatcctccc agtcgttggg cgtgcggccg atgcccaagg
    17461 cctccttgga ggcgtatacg aagttcatat ggccgtagcg tgcgctgctg tccagattgt
    17521 tggtgcggaa gcccgcgcgc atcagcttct ggaacagata cagctcttcg ttcgtgcagc
    17581 gtgcggtaat caatccggcg atggcgtccg ggccatgctt ctttcgaatc tcggcaaatc
    17641 ggtcgacgac gtggtgcatc gcttccagcc agggtgcttc cgcgagctgg ttccctttgc
    17701 ggagaagggg ttgtttcagc cggttcggcg agtcgatgta ttcaaaaccg aaccgtccct
    17761 tcacgcagag tccgccatgg cccttggcgg tttcggcgcg gtcgccccac ttattttccc
    17821 acgacaaggg tgacgtcacg cggatgacct cggtatcctt ggtctccagg tacatctggc
    17881 aaccatcccc gcagtaatta caggtggtcg tggtcctttt catctgccag ggcttataaa
    17941 gatatttgga gaacttgttc gtaatggcgc cgaccggaca gaccgccaga caatctccgc
    18001 agaactcaca ggagagcggg agatccccct tggccacgac ctgattgaac ccgcccttct
    18061 tcatgaattg cagcgcatcg atcatcaaca cgtctttgca gacgttgatg cattccgcac
    18121 aggcgataca gcggttcatg ttgaaatcga ggacgaggct gcgcgtatct tcaggaatga
    18181 acttttgttt ggcgttggtc agatttgtca cgccgtgctg gaaggccatg tcttgcaatt
    18241 cgcaatggcc gtcggcatcg cacacagggc agtcgagcgg atgtacggag agatgtttct
    18301 cgaccgcttt cttgcgggcc tgaaagagat cgtctccctc cgtcctgatg atcatgccgg
    18361 cggtggcttt cgcggtgcag gaacgaaccg gagccttttt cccttcctgc atgaccaggc
    18421 acataccgca ggagccgaat ggatcaaatg tgtagtggta gcacatggcc ggaatgattt
    18481 tgcccgtgct ggagatgacg tcgtagagcg agacgccgtc tttggcggtc acggtcttgc
    18541 cgtcgatgtt caactcgatc gttgcggctt cgacgtctgg attggttgcc ggtttcagtc
    18601 ccataatctt tcctcagtgc tgagttttca gtggggcgcg ctccattgtt cgcgatcaga
    18661 actcacaacc ccgcagccag caattctcac cgatcgcact cgcccatgac gatgtcgtac
    18721 gttccgaaaa gggtgatcgc atccgcgatc atatagcctc tcgacatgtg gtcgaaggcg
    18781 cccatatgga taaacgacgg ggcgcgaatc ttcagccggt aaggtttgcc gcctcccgtg
    18841 ctcacgatat aaaaccccag ctcccccttg tgcgcctcgg ttccgcaata gatctctccc
    18901 ggaggggcgt tgaatccctg agagaaaagt ttgaactgct gaatcatgga ctccaagttc
    18961 gtaaagacgc gttccttggg cggcaaggtc acgctgggga catctgccat gatgggcccg
    19021 tcctgcatct gttccagaca ttgtttgatg atcttgacgc tctcgtacat ttccatcacg
    19081 cgaatccagt accggtcgta ggtatcgccg ttcttgccga gcggtacgtt gaattcgcat
    19141 ttcgggtagg ctgaataggg ctcggctttg cgaaggtcat agtctacgcc ggagccacga
    19201 agcgttggtc cgctgaggcc gaagctgaca gcgtcctcgg cggaaatgac cgcgacacct
    19261 ttggtgcggg ccagccagat tcgattcttc tccaggaaga ccacgtattc gtcgatcttc
    19321 ggcgggaagt actcgaggaa ctgcttgagc ttggcgagca gcgacggggt gaaatcccgc
    19381 tccacgccgc cgatgcggta ccagctggtc gtgagccgcg cgccgcagag ttcgtcgaac
    19441 caatccagga ggatttcacg atcgcggaag caatagaaga agacggtcat ggcaccgatg
    19501 tcgagggcct gggtgcccag ccagaactga tgaccgataa tccgttgcac ttccgccacg
    19561 atggttcgaa ggtattcggc gcgctccggc accgtaatgt tcatgagttt ctcgaccgcg
    19621 cggcagtatg cgaagttgtt gtacatcgcg cagacataat cgaggcggtc tgtatggggg
    19681 atgaactgat gataagtccc ctcctcggcc aacttctcga cacctcgatg gagaaatccc
    19741 atgacgggag tggatttcac cagccgttcg ccttccagtt cgaggatcac cttgagaacc
    19801 ccgtgggtgc tcgggtgctg cgggcccatg ttgagcagca actcctctgt ccgcagcgtc
    19861 ggaagcgtct cgctctccgg gtgcgtaggg tcgaccttgt aaacggtcgt tctttggtct
    19921 tcaaacgcca tggttcagct ttctctaccg agccgtttcg tccagaaact caaacgtgtc
    19981 gcgccaaccc ctgccccgaa gcggaaagtc tttgcgtaag ggataccctt ctgtgtaatc
    20041 gtccggcatg aggatccgtc gaagatcggg gtgatggcga aaacggattc ccatcatgtc
    20101 gtagacttca cgttccatga agtcggcgcc catccaaata tccgtgagcg agtccaccac
    20161 acaatccgac tcgggcacac gggtcttgag ccggattcgg tggcgcttct tgatcgaata
    20221 gaattcgtag acgacttcaa agcgttcttc atcgtccggc cagtccacgg agctcacgtg
    20281 gacgatgtag tcgaaatcca tagcagaatc gtcatgcaga aagagcgcca cttcatggag
    20341 tttgtcgcgc gtaaccgtaa cggctaaatc gccgcgccac tctaccgcct tcacaaagcc
    20401 ctcggtgaag ttctcttgga tccggctggc cagctgttgg agcgacatgg ataacgccct
    20461 agaccttcag actcgtcttg acttgttccg gttgcttgac gaacacccgt ttctgcatga
    20521 tccgctcttg taacttgaga atgccgtcga agagggcttc gggagtcgga ggacagcccg
    20581 gcacgtaaat atccaccggg acgaatcgat cgacgccctg tacgacgcta tagctgtcat
    20641 aaatatttcc ggatgtggca caggacccca tcgcaatcac gtacttgggt tccggcattt
    20701 gatcgtagat ttttctgatg acgggcgcca ttcgtcgaca tacggtcccg gcgacgatca
    20761 tgagatcgga ttgccgtggc gaggcccgaa atacccctgc cccgtaacga tccatgtcat
    20821 agcgcgagga gacggcagcg atcatttcga tcgcgcaaca ggccaaacca aaggtcatcg
    20881 gccacaagga gcccttgcgc gcccagttca cagctttttc gagtgtcgtg gtaatgacgt
    20941 cgggcgcgcc gtctttctcc tgccggccca gctggattag tcccattcca aagctccctt
    21001 acgccaggcg tacacgtacg ctacgaggaa cagcgcgata aagaccaaca tttcgatcaa
    21061 accaatgacg ctgaccttat tgatgtcgtt gaacacaaca gcccagggat acaagaagat
    21121 gacctcgata tcgaaaatga cgaacaacat ggcaaagatg tagtagcgga ccgggaaagg
    21181 catgcgcgca tcggaaaagg gctcggatcc gcactcatag gcggacagct tctctggttc
    21241 cggatacttg ggttgaacga gatagctgat gagcagcgtg acgacgccga aggccaacgc
    21301 cacgaaaatg aataggagaa tcggaaaata cctggtcaaa tactcaagga gtaactcgag
    21361 accgctcatg gaaatgacct ttcaacacct tgagttgcaa aggagagcgg gatcttagac
    21421 gctggcttgc cgtgatagca agcgtacaaa ggacctacgc ctctagaccc gaaagtccta
    21481 ggggtgacgt cagcacccaa aacaagcagg atcctgctgc ttcttatcgc cggagtatct
    21541 gggcacccaa tgcggaatgc agactacttg ggagcggatg gaataggttt ggaagccatc
    21601 agggattcgt ccattctgag gtgcacggga gcgtcctgat cggtgcgatc ccattcggac
    21661 aacaggggtg agaatacctt ggccttctta cgcctgtgaa tctgagtttc gaggcgcggg
    21721 cgattcgaat cttgcggttt ctttgaaggt gtaggcatgg gaccgctgct gatgatcgct
    21781 gggaaaagag cttaatcggg cagttgcacc ttaacactgg accctatggg ggtcaagaga
    21841 ggcaagaccg caatattctt gacaatatgg gactatttgc tatggttttc tcaggaacat
    21901 ttgcctcaac aagaaggacg gccaccatga tcatttacgc ctcgaagttc accggatgtt
    21961 tgttgtgtgt aattttgggc ctttccggcc tgcaagccgc ctgggcggag gatcccgaga
    22021 gcgttcgttt tggcgaggcg gcacgttcct ttgctgccgg atccattcaa caatcgacgc
    22081 cgcaggttgg cgtggtgaac gtcattacgg gggacaatca gaccgtcggg aatcgaatga
    22141 gactcgggca aaacgatgtc ttttatctca agctcaagaa ccccggtgat gcggcggtgg
    22201 gcgatattta cacggtgttc aagcgggcac gcaaagtatt ccatcctggg aatggccggt
    22261 acatggggta tctggtcatt cgacttgcca tcgtccaagt tgtcgatgtg gacaaagagt
    22321 taacaacggt ccaagcgatt cgctcttatg gatctgttgc acctggtgac ccggttatga
    22381 agttctcgct gccgacggaa ggtgactcaa gctccgctcc cgtttccgat gatgtgatgg
    22441 gaatggtcgt cgagattcag gccgatatgg gaatgacctt ggtggcgcag cataattttg
    22501 tgtatctcga caggggttcc gaagatgggc tgaaaaccgg cgacagaatg gacgtcgttc
    22561 gcaccggagg gggacttccg ccccgcaaag tcggcgaagt gagaattcta tcgaccgaac
    22621 cgcgtactgc cgcggctgtg gtcacgaaat caacttcccg cattatgaaa ggcgatcggt
    22681 tccggtcgag agtgcagaca ggagagccag ctccgactcc cgtttcgcag ccgggcagac
    22741 aatcgctcaa cgagtccccg aatcaagctc aaaatgccgc ccgtgagact cgttatagcc
    22801 tgagcgacct catcaaacag ttgcgctatg attcaggtga cgccaggatc aaacccgaag
    22861 ggtatcaagt tctggaccaa ctgatcgaac agctaaaggc cgcgccgcca gagcagttga
    22921 ttcgggtcga aggccatgca gacaacatgg agatcggtcc gtccctgaag tcgatctatc
    22981 cgacaaactg ggatctctcc aaggcccgtg ccgggggcgt actgcgctat ctcgtcgaga
    23041 aaggcggtat cgactcggcc agaatatcgt cgatcggcta tggcgacagc agaccggtgg
    23101 tgagtaatgc cacggaaacc ggccgccaga agaaccgccg agtcgatatc gtgctctatt
    23161 cgcctgagtc ggccaaggcc ccttccgagc aggcgatgaa gccaactgaa gccgtcgaga
    23221 gcggctatgg ggtcgccggc gcggagcccg ataatcagaa tgcgtcagct cctgctacag
    23281 tgaatgtggc gccggctgat tcggccaagg tggataattc ggaggcggtt ccggcgagtt
    23341 cggttccgga tgcgaatcct gcggttggcg atcaatctac gccggcaaag ctaatggatc
    23401 agcccatcgc tccttccgaa aagtaagcgc cgtctcgtgt caagcggtgg agttccgtgg
    23461 ccggccatgg acgctccacc gcaagacgct tcttgccagt cgcgttcgtc cctccgttgt
    23521 tccccctttt cccctactgc tagaatgccg catgtctgtg aaccacttgg catcctccga
    23581 cagggaatct gatccacttt ttgcggacgt catcgtcccg cgccacctgg ccggcccatt
    23641 tacgtatcgc gttcctattc ctttgaaaac agttctccat gtcgggcact tggtccttgt
    23701 accgtttggc cgttcggtga ttcaaggagc agtgatctcg ttgaccggag taccaccccc
    23761 gaccctcgct ccggaacagc tcaaagagat ccgaactctc gcgaccaacg gccaggcaac
    23821 cgaagtccct cccgatcttc ttcaactcgc gaaacgggtg gcggagtcct atgtagctcc
    23881 atggggccag tgtctcaggc tggtcatgcc tcctagcggg gttgtagggg cggccggtgg
    23941 ccgctttctc ttgactcaaa agggccgtga cgcgcttgca gccaagacca ttgccatgtc
    24001 agatgagcgc atacttctcc agcggttgaa gaagagaccc ctcggcatca aagaagctac
    24061 gcttcgcaaa agcaaagatc gaggtgaagc tgcgctgctg gaatcgctcg tcgaacaagg
    24121 atggattcag gagatccagg agcccgcgca acctcgcaac caacttcgct cgtgtatcgc
    24181 aacccagtcg gaccgcgact tgtatggcgc cgatattcgc ccgacgaaaa tggactcgcg
    24241 ttacccaaaa gattgggagg atcgaattgt tcaggctatc gagcggcgcc gggcgtcaca
    24301 gcttctgata caagctccgg ctccccagcg gcttgccttg cttcgtgaag gcatcagacg
    24361 aactgtccgc attggacgta tggtactcgt gattgtcggt gaagcggagc gagcggaatc
    24421 cctcaccacc gccctctcgg aacacgattc agtggtcacg gcctgccttc acagtggctt
    24481 atcggagtat caaaaggctg acgtgtggcg acagattcga gagcagcgca tccacgtcgt
    24541 ggttgggacc aggtcggccg tcttcctgcc gctttcttcg ctcggcctgg tctggatcga
    24601 acgggatgag gatcctgcac tcaaagaacc gcaggagccc cgattccatg cgcgggaagt
    24661 cgcgtggttt cgagcccagg aggccgaagc cctccttgtt gtaggctccg cgcatctctc
    24721 cctggagacg cttgcgggtg tcgaagaagg gtcacgtctg ctccggatcc ccttgcagcg
    24781 agacgagagt cctaggattg aggtggtgga tctgcgtggt caggatcgcg cgatgatcct
    24841 gagtcccttg ctgattgacg cgacgcgcga ggcgcttgcc agacgggcag gagtgctgtt
    24901 gtttctcaat cgaaaaggct atgccggtgc gctgatctgt cgagattgcg gccaggcacc
    24961 tcgctgccct gcttgtcgtg tcgccatcgc ctactatcga cagaagggtt cgctgctctg
    25021 tccctattgc ggaatgactg catcgattcc cgacctctgt ccgtcctgtg caggccctcg
    25081 gctgcggttg atcggagaag gaacggaacg ggtggaagaa gacgtgaaac ggttatttcc
    25141 gcaggcagcg gtcttgcgga ttgatggaga gacgatgcat aaaccgaaac aggctgccgc
    25201 gctctgggac cgcgttcaac aaagacagtg ggacattctt gttggaacac agttgctctt
    25261 gcgagatgat gtggtgccgc ccgtcggggt tgtcggtgtc gttcaggcgg acgcggggct
    25321 gaaccttccg gactttcgcg cagcggaacg cacctatcac ttgttagccg atgccgtagg
    25381 ccttgcccat ccatcctcaa gcggaggccg agtgatcatc caatcctatc taccctccca
    25441 ccatgctatt caggcggtcg ctcagcagga cgaaacgatt ttcagagccg aggaactgac
    25501 tcaccggacg actctcggct atccgccggc cgtccacttg atcgtgcttc acatatccgg
    25561 gagtcaggag aaaatcgtcc aggaggccgc attggcatgg gttactcgcc tgcgtctacc
    25621 tggcgtgaac ggaaaggcgg cgcagcaggc ctccggtgag gacaaggcga cggagcaggc
    25681 cgaaagtctg acaatcttgg ggccggtgcc atcgcctgtg ccgaaactcc gggggcgata
    25741 tcgtcggcaa atcttgatca agtcccgtgc gcgcgatgcc gcagttcgag cggtgcgcag
    25801 gacggtggag gaacttgaac agacctatcc ctcgcggctc gtaaagtttg acgtcgacgt
    25861 tgatcccttg gaaatgtggt gacagtcagc gcttcttgcg cggatgaatt ggagccgtcg
    25921 gcggggattg ccttgaagac tttcctggca attccggtga ggaaacggac tgttctcccc
    25981 cagcccgttt gaacaaaccg tacgagaagg caatccagaa cacggctgaa aacagtccca
    26041 tgaggacggc ggagagaatg gtggccattt gctcatcgga cggttgagca gcggccgact
    26101 gaattcgaac catcgtgatg ataggccagg cgaagcattc tacgcccagc aagagggtag
    26161 accaggccca cactaatccg gtcgtgtggc cgcgccagag gagaaaagct gcgacggatg
    26221 ccatgacaag gcctgcacct ggaatcgaga gctgatccca tgccagccat gccccgaccg
    26281 tgacgacgag gctgcccaat cccgcgttga gtcgagaggg ccaggttgca accatgaaag
    26341 aacactttct gctactaggg ttccacagaa ttactcagta gaaagcggat gggaattgtc
    26401 aagggaatca tgcagtgggg cataaatgaa gggaggcagt tatgcttgca aaatggattt
    26461 cgctgcctcg aggagatcct tcatttcaaa tggcttatga agaacacgat gtgcacccaa
    26521 catttttgca acatcaagga tgccaagaat accgatcatg tcgttactgc ccgtcatggc
    26581 aatgatcttg actttgggcg actcatgtcg aatcgttgaa atgctttcca gtccgtcctg
    26641 atctggcatg ataatatcta tgaccacgag gtccgtcggg gtcagtcggt attgttcaag
    26701 tgccgctgtt ccactgcttg cctccaagac ctgatagcca gattcctcga acgtggttcg
    26761 aattagctgc cgaacttggt cttcatcgtc tacaacaagg accgacggca tagcatccct
    26821 cggtttcggt catgacctta aactgggaaa agtatcgcac caaaacatcg agctttttct
    26881 gtcgatcaag ctcccctcaa cgactctaac atctcgctgc ggcgcgggca acatgtcaag
    26941 gatttgggat acgccgaaca gataacaggt atcgttactt ccgcatgttg gaagaagggc
    27001 aatgcaggtt cttacgcctg cctttttgca aagaacgcag cagcttgcgc gcgtcgcgag
    27061 atatgcaact tctgaaacat atttgccagg taattcttga cggtcttgtc actcaattta
    27121 agggatgtcg cgatttcctt gttagtcaaa ccttcggcga ccaaggccag gaccttttcc
    27181 tcctgaggag agagcgaaga agtccggacg gggccatcgg tggtcgggac ggccttgatc
    27241 cattcgagtg cccgctgggt gaccgtggga tcgagaatcg accgaccgga tgctacagtc
    27301 cgaatggcgc gaatcagcgc ggcggaatcg atctccttga ggacatagcc tgatgcaccg
    27361 gccagcacgg cggcaaggac agagtcatcg tcggtatagg atgtcagaaa gatgacccgt
    27421 gaattgggat ggtctgccag aatttccctg cacgcttcga ctccggaacc gtcgggaaga
    27481 cggacgtcca tcaagatcac gtccggataa agccgcttgg cttccttggc cgcttgtgcc
    27541 ttgctaccgg cttctccgac gatggtgatg ccctgattgt tgtgcaagac agtgcgaagt
    27601 ccgacacgga ccacttcatg gtcgtcgacc aacagcagcc gtatgggggc gggtttaccg
    27661 gatatcatgg gctaacgtca cggagaaatc gtacaggcag aatgtagagg tcccgatcgg
    27721 aagcggtaag gataagaaga gtcccgcggc gagtgcgagg agagacgctc cgtcagaaag
    27781 cctgctctca acggcaagga gccctaccgg cgagttcctg gtcgagcaca tgaatgttgg
    27841 tgccgaaggc gggacttgaa cccgcacggc ttgcgccaca cgcccctcaa acgtgcgtgt
    27901 ctgccattcc accacttcgg catggtccat catggtcaag aatgagggag cggcattata
    27961 gaagtaggcc aaaattcttg tcaatcgagc gcacgttcgg tagaatcggc ccctcacatc
    28021 gtgaatcggc tccgttcacc tccatgccaa gtaaccgctg gacgatctgc acatgattcc
    28081 aacgaaagcg ctggccctac cttgcactga gacacgagtc gcggcatttt ttgacgtcga
    28141 caatacgctg ttgccgggcg aagccagcga agtgcgcttt ttcagaacgc tctggcgacg
    28201 cggggtcgtc ggctggcccg aattacgcga gagtgcctgg tggcttctca ggcaggtgcc
    28261 gccgctgtcc cttcagccgc ttcgcgaacg caaagtctat ctcgccggca aaccggtcca
    28321 agtgattgaa ccgttgggag aggaattctg tcgggaggag ctctgtccgg tcgtggccgc
    28381 cgacggattg aggaggatgg aagaacaccg ccaggccgga cacgtcatca ttctgatcac
    28441 cggttcgctc gattttctga tcgagccgat tgccagcgcg ttgcgggtcg atcggtgcat
    28501 cgcggtgacg ctcgaagcgc gtcacggcct ctatacgggg caactcagtt ctcccctccc
    28561 atacggcgaa ggaaagcgcc gactgattca gcaactggcc tccgaactcg acctcgatct
    28621 acgaacctgt tttgcctacg gcgacagtcc tggtgatcgg gagattcttg aatgtgtggg
    28681 aaatccaacg gtggtgaacc cgatccgcgg catggcccgc atcgccaagc gaaagggctg
    28741 gccgatacag cagtggcggt agccgccgcc acgcttaacg agtgtatgaa gtgtatgatc
    28801 agctgaaacc cacgccatgc gcataaccga aatcttccat agcatccaag gggaatcgag
    28861 ttacatcggg cagccctgtg tgttcgtacg gctgaccggc tgtccgctcc gatgcacctg
    28921 gtgcgataca gaatacgcct tctatggcgg gcaagaatgc tcggtggacg aggtgctgga
    28981 acgagtcggt tcgttcggct gccgattggt cgagatcacg ggcggcgaac cgttggcaca
    29041 gccggaagcc tttgcccttg tgaccaagct gtgcgatgcg ggttatacgg tgctgatcga
    29101 gacgagcggg gcgatggata tcgccgtggt ggatcagcgt gctcatgtga ttcttgatgt
    29161 gaaatgtccc gggagcggaa tgacagatcg aatgtattgg ccgaatctcg gcaagctgac
    29221 catgaaagac gaggccaagt ttgtgctggc ggatcgacag gactatgact gggcccgcga
    29281 gatcatgagt cgatatcagt tgactgagcg ttgtgtggtg ctgttcagcc ctgtgttcgg
    29341 acgtcttgaa cctcgacagt tggccgagtg ggtgttggcg gatcgtctgc cggttcgatt
    29401 tcagctgcaa atccacaagt ttatttgggc gcccgacatg cgcggtgtct agcgggatga
    29461 aagacaagaa cggaacagga ccgtccacgg tgacgtttcg ggaacggcga tcgacgagca
    29521 cgaaggagac atgaatgaac atcatgcagg ttgatctgaa ggttgggcgt gcagagggac
    29581 aatcggccga ggtgttggcc ttaacccatt atgaggggga aggcctcaat aagcaggacg
    29641 cggcagtgat cgacaagcta ctgggcgggt ccgtccgaga gctcgtacag acgaaagaat
    29701 tcgaaggcaa ggcgaatgaa tccctgctgt tccatacgca gggcaaagtg ccggccaggc
    29761 gactcctttt gatcggactc gggaagaaaa aagatctcac gcttgatgtg atcaggcagg
    29821 cgctggggca agcggccaaa cgcgtgcgcc aagcgaaggt ggactcgttt actgttgctg
    29881 tgccagtcgt gattccgaag gggtactcgc ccgttgaggt ggcacaggcg atgactgagg
    29941 gagcgatcct gggcgggtac cagttcacca cctatcgcac cactgatgac agcgtgggga
    30001 aagacgtggg acagatgacg ctcctggctc agcacaagaa ccaggttcgt caattggcgg
    30061 agggaattcg ccgcggggtg gcgacagccg aagcgacggt gtttgtgagg gatctctgca
    30121 atcatccttc gaacgtcatg acgccggtcc gtatcgccaa tgaagccaag tcgattgcga
    30181 agagcgaagc gctcacgctc aagattctcg agcaaaaaga gatggagcag cttgggatgg
    30241 gtgccttatt gggtgtggca cgcggcagtc acgagccgcc gaagttcatc atcctcgagt
    30301 acaacggggc gaagaaaaaa gacgaacgtc cagtcgtgct cgtcggcaag acgatcacgt
    30361 ttgacacggg aggaatctcc ctcaagccgg ccgagaatat ggagcatatg aaagcggaca
    30421 tgacgggcgg cgccgaagtg ctggcctcga tcagggcggc ggccaggctc aagcttcccc
    30481 tccgtctcat cagtattctg ccggtggcgg agaatatgcc cggcggacgg gcgatgaaac
    30541 cgggcgatat cgtcaagacc ctttcaggga agactgtgga agtccaaaat accgatgcgg
    30601 agggccggct gatcctggct gacgccttgg cctatgcgca acgctacaat cctgctgctc
    30661 ttgtggacat cgccacgctg accggtgcct gcgtcgtggc gctgggccag ttcgccatcg
    30721 gcatgttcgg gactgaccag aagctgaagg aacaggtgcg caaatccgga ctgaaagccg
    30781 gtgaacgggt ctgggaaatg ccgctctggg atgaatattt cgagcagtta cgcagcgacg
    30841 tcgccgatat gcgcaacatc ggcggtcgtg gcggcggaat gattacggcc gcgctcttcc
    30901 tcagcaagtt tacgggtgac tgttcctggg tccatctgga catcgccagc accgattgga
    30961 gcgagcggga gcgggcctac attcccaagg gaccgaccgg catcgggacg cgtttgctta
    31021 tccagtactt gatcgatcgg agcctgtgac gtggcgcgga ttgagttgac ctcgcgcgag
    31081 aaaattggcc agctgttcat gatcggtttc atgggcacgt ccgtgacgcc cgaactggtc
    31141 tccttcatca aagagtacaa accgggcggg gtgattctct tttcacgcaa tctggaatcc
    31201 gtcaggcaga tcgtcgaatt gaccaacgac ctccaacgat gcagtcctcg atcgccgctg
    31261 ctgatctcga tcgatcagga aggggggcgt gtctctcgct tgcccagggg ctttaccatc
    31321 ttcccgccgt gcgagctgtt gggacgctgc aattcgagcg aactcgccta cgcggcagcg
    31381 gcgacgatcg caaaagaact gaaggcggtc ggggtcaaca tgaacatggc tccggtgctc
    31441 gatgtgaaca gcaatcccga caatccggtg atcggcgatc gggcgttcgg gtcaactcct
    31501 gatgtcgtca gcgagatggc attggtcaca gccgcggcac ttcaagacaa taaggtcgtc
    31561 gcctgtggaa agcattttcc cggtcatggc gataccaacg ccgattccca caaagagctt
    31621 ccggtcgttg aggcctcgcg tgaccggcta gaagtcatcg agttgccgcc gttccgtcgc
    31681 gcggtgacgg cggggattgc aaccatgatg acggcccatg tcctgtacaa ggctctggat
    31741 gatcaactgc cggcgactct gtcccccacc gtcattacga acctgttgcg cgaagacctt
    31801 cagtacgacg gcgtggtgct gaccgatgac cttgagatgc atgccattgt ggaccactac
    31861 ggcgtcggtg acgcggcggt tcgcgcgatt ctcgccggct gcgatgtgtt gttgatttgc
    31921 aaagagcgcg aacgcgaggt gtccgcgttc gaggcgattg agcaagcggt agcctcggga
    31981 acgatctcca tggaacgctt gaatcaatca gtggcccgaa tccaacgggt gaagcagcgg
    32041 ttcctcttgc cctataagcc ggctgtcatc tctgatgcga agctggtcgt gggctgtcgc
    32101 agccatcagg cgttgttaca gagcatgcag caggccaagt cgagactgga gaaggtcggg
    32161 gcgttcagcc gcttgtgagt ctttcctgtt gaaccgttga cgggagcgaa cgtgggtgtg
    32221 aaaaaaggcg atctcatcga cgaacgaaag cggcatccgc atgcctgcgg tttggtcgtg
    32281 aaggtaatgg gtggcggtga gggattcgaa aagatcatct gctgcgatca cgatttgacg
    32341 gaagaggatg tcgtgaacga gatgggctca cacagcgggc ggagaaaggg acaactgccg
    32401 ccggccgtca tactcgacga aaagaaactc tatccggact cctgcggcct ccgcgtgatg
    32461 atcatggacg gcggggccgg gttcaaagaa atccgctgct gcgggcattc gctgaccgcc
    32521 agttcgatgc gagagcttca atatggcaag atgcggggat ctgaaccaga cgtgacaggg
    32581 cctgcgggaa cagcctgaag gccttggttg gaggaatgat ggagcaccga agagagctca
    32641 tgaaacgttg gtgcggcgtc atggaatggc tcgatcgtct cggatacctc accgctggat
    32701 ttagcctgtt ggtcctggga atggtcatct tcgctcaggc ctggtacgtc ttcgcgacca
    32761 aggcaggcca ggcgggactc ctgccatccg gccttaaact gttaaatgac ttgctgctgg
    32821 tcatcatctt gctcgagttg ttccgaaccg tcataaggtt tctgcagacg gaaatcctcg
    32881 acctggagcc ctatcttgct gtcggtatca ttgcctgtac gagacgagtc ctcacagcca
    32941 gcgccgaact gtcccacctg ccgagcatga ccgagacgca attctaccaa tacctcatgg
    33001 acgtcggttt gaatgttacg gtgatcatgg tcctcgtggt gggggtcttc ctggtccgta
    33061 aacgccctgc ggagtctcct gccgtctccc cgtagctgtc ctcctttcga gttcaagctc
    33121 ttggagatga agaaggggag ccgactcctt gccctcccgt tccctcttat ggcatcatgc
    33181 ctctccctgg agatcggcat ggcgaatttg ctcgaatacg tcggctgtgt tcatatctat
    33241 ctcggcccct acagaggcaa tcccatcgcg ttgtacttga agcgctctga caccagctgc
    33301 cagatcggcc cgaaggtcta tccctggaac gatgtggtgg gaacgggtga aacgccaaat
    33361 aaggccgctg cggatttcga ggacaagtgg aaggccaagg ggctatcggc cgatatgtat
    33421 tccggtcctt cttgggaagg gggcgtcaag ccggagaagc ctgctccccc caagcccccc
    33481 gctgccccca agccggcaac ctcgactgcg gcggggtcgg cagggcaacc agccgcttcg
    33541 ccagcaccag gatcagctcc tgctcaaacc actgcagctc ccacacccgg tcctccggcg
    33601 aaacctacgg gcgagtccag ctagcatccc aattattatc ggagaggtcg agcggcgagg
    33661 ctggcatctg gagattgtcc agccagccta ctgtgtttga gaccgtagcc taagtagacg
    33721 gctatgccga taaccgtcca aattccgaat ctcatccagg tcagctgagg aagttgccac
    33781 atgagaccga gacaggccag cgttcccaga atcgggacaa tcggcatgaa tggaagccgg
    33841 aacggtcttg gatggtcggg cttggtgtac cggagtatga ttacgctgat gcagaccaag
    33901 acgaaggcga agaacgtccc gatgttcgtc atatcagcag cttcaccgat cggaatgagc
    33961 gcagccaggg tggcaaccgc gataccggtg agaatggttg cccgatgcgg tgtgccgaac
    34021 cgcgggtgga ccagcgacag ccagggaccc agcagacggt ctcgcgacat ggcaaagaag
    34081 acgcggatct gacccagcat catcacgacc agcacactgg tgatgccggc cactgcgccg
    34141 atcgcgatca ctgccgctcc ccacttgaat cccgccagcc ggagcgcttc tgccaccggc
    34201 gcatgaatat cgatttgcga agaaggaatc aatcccgtca gcacggctgc gacggcaata
    34261 tataagatgg tacagacggt cagcgaaccg atgatcccga tgggaacatc ccgtttggga
    34321 tttttggctt cttctgcagc ggtcgagacg gcatcaaatc cgatataggc gaagaacaca
    34381 atcgccgccg ccgctcccac tccggcgaaa ccctgcggca tgaacggagt ccaattgtcc
    34441 gggttcacgg aggtgcttcc aatgccgatg aaaaagagga tcaccgcgag cttgatgacg
    34501 acaacgatcc cggttgcgcg tgcgctttcc ttgattccca ccacgaggat gactgtcacg
    34561 agcaaaacga taattgcggc cgggatattg gcgatccctc catcagatcc gccaggtgga
    34621 tgggtggccc aatggggcaa ttcgaggccg gcaagtgtga ggagattgtt aaagtagccg
    34681 gaccagccga tcgccacggc aacgcaggcg acgccgtatt caaggatgag attccatccg
    34741 gtcagccagg cgagcatttc gcccagcgtg gcataggaaa aggtgtaggc actgccggcg
    34801 acaggaatca tcgcggacat ttctgcataa cagagcgcgg ccagcgcgca ggtcaacccc
    34861 gacaaaatga aggagagaac gatgccgggc ccggcgcctg gtcgatgggt atcgccgaca
    34921 atggcggtgc cgatcaatac gaagatgccg gtgccgataa tggcgccgat tccgaggcat
    34981 gtgaggtccc aggccgtaag cgttttcttg agtcgatggg tgggatgatc ggcatcggag
    35041 agaatctgct cgatgggttt tgttctgaac aggcggctga tcactgagct tcccccgata
    35101 gcgggagggg cgactgctga ggggctaagc gcgtcacgtt ccatatctca tcaagtatag
    35161 caggggcttg caggccgccc ctggcttgca cgcaagacgg catcatcgcg aggaaggtcg
    35221 acggcgggcg gcgcgaagga acgcctcgaa gagctgccgg tgcaggcggt atcgctcaaa
    35281 gagaaactca gggtgccact ggatacctag aaaaaagcgc tgcgtcggcg attcaatggc
    35341 ttccacgatt ccgtccgggg caaccgcgct ggcaatcaag gaaggcgcga cgttctttac
    35401 ggactgatgg tgtgagctat tgacgcgcat cgtggcagcg cggacgacct tacggagcaa
    35461 actgcccctt gcgacggtga ctgaatggct gagcgtcgtg gcaggactcg gttgtcgatg
    35521 ttggagaggc ttggaaatct gtgaggggat atcctgatag aggctaccgc cgaatgcgac
    35581 attcatggct tgcattcctc cgcagattcc gagcacagga agatcggctg ctcgggctag
    35641 ctgcgccatc tccaattcaa acgtcgtgcg ctgctggttc atgacgcgga atttatagcg
    35701 ttgccgttca ccgtacagtt caggtgccag atcaggacca ctcccggtca gcaacagccc
    35761 atcgatgctt cccaggaggc agcgtctggc gctgaggtct gcgacgagcg ggaggacgat
    35821 agggatcccc cccaattctt cgatcgcgcg aacatagcgg gcgcgcagga agtaggtggg
    35881 ctctcgccca ccccattctt ttcggtcacc ggcgttaaaa tctggcgtca cgccgatgag
    35941 cggtttcatt gcttagcgca caggctccat cgtcggagac ggtgccgggg cgggggcctc
    36001 tgattcgggg acgccgagga agcggatggg cttgtcgccc cgtaggtgat cgggggtaat
    36061 gatgtaggtg ccgtacatgc tggtcgtcca gacactttta gccgcattct cattgcctgc
    36121 ggtaaaggca taggtcaggc cccccacgat ccctccacag atagcgaagg ccgctttgag
    36181 tgggagataa ataagagtgg acaggccagc ggctgcctgc atgcccgcgc tggatgccga
    36241 gccgccttcg ctgccgtcac cggtggaggc tggcgcggcg tcctggctcc aggccgaggg
    36301 agccatcgtc accgtgccta gagtgagaat gatggtgagc gcgaccaact tgaagagggc
    36361 tgtcgcggca gttctggata aaaacgcgta agaattcacg ggtaatcctc cttcctcaac
    36421 agagcgagct gagcggcatc tggtggcaac ggagcgcttg ctcacgattt gggatcatag
    36481 gggttataac aaattcacct ctgaatgcaa accccctgcc gcgtgaggcg gttcatggcg
    36541 agagggtctg atcgttcggc aagagatcga gttcagtcgc aacatacgac gtgatgaatt
    36601 cttgtcttgt tgtcacgctc gttctcaatt cctctacgaa tctgtcggct gtgagacatt
    36661 ggttgtcctg tgtgaggccg gattgcaaca gcagatccaa tttacaccag cagatagctt
    36721 ggatgaaaag ttcctgccct ctgtccctat aggatgggcg atcttccaga gggactgtca
    36781 gaagaatatc gctgagccag tgagggaatc cgattgcgga gatgatggca tctatctgag
    36841 gctgattgat ctcgacctca acctggaccc ctcccttcca gcttcgtttc ttgacgttaa
    36901 atactgtgga gtaggtgaag ggtttcccct ccaccggttg aggccccaca aacagggtca
    36961 cgcgaaactg agccgattca ggagaagaat ccgtcgacat ggcgcgcatt gtaccatgtc
    37021 gatgcgcgcg aagcgtgccc ttcgttgacg cgttggcagg gcaacgataa gatgccgatc
    37081 atcatgacca ccatacagcc ggagagagtc gctgccatcc agcggacaat gcgcgaggag
    37141 ggtacgatcg atggatggct cttttacgat tttcgcggca gcgatccctt gagctatcgg
    37201 gtgttgctcc tcgatccggc gatgcatgtc accaggcgct ggtactactg gattccttcc
    37261 gagggcactc cacggaaact cctgcatagg atcgagcctc acgtgttgga tgggctgccc
    37321 ggcgagacgg atctctatgt gtcctgggag caacaacggc agctactcgc cgggctcttg
    37381 aatggcagtc atcggattgc catgcagtat tcccccttga acgccgttcc ctacgtctcc
    37441 cgtgtggatg ccggcacgat cgagttggtc agaggttgcg gggtcgaggt tgtcagttcc
    37501 gcagacctga ttcaaacctt cgaggctgtc tggaccgatc gacagctgga gtcacatcaa
    37561 tatgctgccg cagccctgcg tcgaattgtc gacgaggcgt ttgggcacgt tcgtgacgcg
    37621 gtgacgaaga gccgcggact tacggagtat gggttgcagg aattcattct gaagcgcatc
    37681 cacgaggccg gcatggtcac atccagcgcg ccaattaccg cggtgaatga acatagtgcg
    37741 gatccccatt atggtcccaa ggcgaccggg tccgctgaga tcaggtgtga cgacctcgtc
    37801 ttaatcgatc tctgggcgaa acaagcggct gcgggctctg tctatgcgga cattacctgg
    37861 actgcctatg ccggccgcca ggttccggcg aggcatcggc agattttcga tctcgtccgc
    37921 cagggacggg acgcggcgct aaattttgtg caggcgcaag tgagggcggg acatcgcccg
    37981 tttggctacg aggtcgatgc agcttgtcgt gacgtgatcc gggcagctgg ctatggtgat
    38041 cagttcctcc atcgtaccgg ccactccatc ggtgaagagg ttcatggcaa tggggccaat
    38101 atcgacggtc tcgaaaccca tgacaccaga cggctgatgc ctcatacctg tttttccatt
    38161 gagccgggca tctatttacc cggcgagttc ggcatccgga gcgaattgga tgtctttgtg
    38221 tcggagcgtg acgcgctggt ctttggcttg ccgcttcaaa gcgacctctc ccctctcttg
    38281 tagttgttcc tgcccccatt ccttgacagt gccgagcgcc gacctataga gtgattgctg
    38341 ttcatcatga gtccctaagg gcgcccaggc ccttgcgttg cagggaaagg aaccaggcca
    38401 tgttcggaac gatggggatt tcagagctga tcatcattct ggtcatcgtg ctcatcatct
    38461 tcggcgcggg aaagctccct caaatcggag aaggggtcgg taaagccctg aagggtttca
    38521 agaaagaagt gcacgacatc ccacagcctg agccggttca ggctgaggcg gtggagacag
    38581 tgcctcctca agtgcaagct cctattcaac aggctcagcc cgctcctccg acccctgcgt
    38641 ctcccgccgc tgcggccccc aaacccacag ctccttacac accgggtccg gagctgactc
    38701 cgggaacgac cgctgccttg atgtataaga ttgccgcgca accgcaggct cctcgtccag
    38761 ttgcggccca gggagccgcg cctgttctgg ctgctgatca aggccagcga cctccgacca
    38821 tggaggaacg tgccgcagcc ccgagcccgg tgacgcgtgc acaatatcca ccgttgcccc
    38881 caagtgcgca gcagaagcca caagtcaaac gtccttcggc aattgtcaac aaggatgcgg
    38941 tggcacgcgt gcaggcccag caggcagcat tgaaggcaaa ggcgtcccag ccgggcggcg
    39001 gggtctcacc tgccgatatg cagagtttag gtgaaggtct tggcgatgcc ctccgcacat
    39061 ttaagcaagc ggtggcggat gttcgcaatt cggtcgatcc cgagatgcgt acgattcaag
    39121 ctgaaatgga tgcagcgcag aaggaaatcc agcaatctat cgaggctgcc agggaattac
    39181 ccgctgtgca ggaagagact cagaagccgg cgtgacatgg tgtcaccgtt tcaggcagca
    39241 tccgtccttg tgtgaaatct tgtcacccgt tctttcgcgg tgggtctatc tccttcttcc
    39301 cataattcta ctctggttgg tcggctgcgg ccaggaggtt cctgcgcgct cggctgagca
    39361 cgccgtggcg cttctcacgc ccctgctacg agatgagaac gctgacatca ggagaacggc
    39421 tgctgagtcg ttggggaaga tcggcgatca ggcggctgtt attttcatcc tgccactagt
    39481 gagcgatcca gctcctgcag tccgtgccgc agcagtgcag gcgttgggcc ggatagctct
    39541 tcccgcgact caagaggtcg tcgacgcact cacccaggca ctgcaagatc ctagtgattc
    39601 ggtcaaggag gccgcatcca tggccattgg cgacattgaa ccagcctcca atcaactggg
    39661 gcttgccgcg agcctggtga gcgctcctga tactcaggtc aggcgcgcga cggttcgagc
    39721 gttgcggcag gtcgatgcca gcctctggct ttccgtgctt gtgtcggcct tacgtgattc
    39781 ggatgcggag gtgcgacaga gcaccgttgc ggcattggga gagtccggtg gaatgagtat
    39841 ggccatagag ataaggaaac ggctcacaga ggactcatca cctgcggtgc gggcagaagc
    39901 ggcctatcag ttggggaaat tcgggggtcg agacgtcaag ttggcgcttg agcagacggt
    39961 tgcgaaggat cccgagagtg gggttcgtcg ttgggcggag ggggagctta agtcattacg
    40021 cgtgagcgat tgagtgcggc gacgagttcg acgagttctt tgcgggactt gtcgtcgatg
    40081 tcggtaaatt ggactcccat accgggaaac aagagatagc gctcgggctt gttacgggtc
    40141 caggcgactt tggctttggc cttgtgtttt tcccacgggc ggtcgggaag ggaaaattcg
    40201 atcccgagtt cggtgcccgg tgccagcggc tggctgcttt cgatgaagaa acctccgcct
    40261 ccgattccac cagtcaaact gtcgaactgc tttccttccg gtgtcgtgta tttgaccttg
    40321 acggcaagcg gagctcgcgg gtgtgcccgg gcatgcgcga atctggtgtc ttcatcgctc
    40381 gtaatgatgc gttcgatgat ttctttccag gaaaccgcac cgagtaatgt tctatccgaa
    40441 ccatgaaggg taatcacttc atgattcgta tcgatctcga gggttttccc ctcatggccc
    40501 tgcgtcgtaa cgactgaaaa cttcatgacc agattctcgc tgtgcggatt gtgaggtgca
    40561 atgttaaccg ttcaccgtca tcaggcagcc ggctggccga cctgctgcag ggtcttcacc
    40621 aattcgagca ctcgatcgcg gatgttctgg gcaatgtcgg taaatcgaac acccatgccg
    40681 gggctgaacg tgtactgatc tgctttggga cagacccagg ccaccacgcc tttcgctggc
    40741 agccattcgt cggggcgctc cggtagagaa aactccattg tgagtttggt cccgaccggc
    40801 aacggggcct gactttcgat gaagagcccg ccgcctccga tcccgccggc ccgactctcg
    40861 aactgttggc cctcgggtgt cttgtatttc acgcggaagg acagagtcac acgcggttct
    40921 gttctggctt ccctggaagc cggaggcttg cggtatgcga gaatctgatc gatcacgaag
    40981 tcccacgaca aatttcctag ctgctcgccg ttgatgccga tgagcgtgac gatctctcgg
    41041 gccgaatcaa gttcgatggt cttccccttg tgccggagac tcgaaaccac aggatatttc
    41101 acgcgtcctc cctctacatg atcacttcgc aagtatagcg cagcattcct cgttgtcgag
    41161 aaaaaccgca gactcgtcat ggggatatcg gtaccatgat gaggctggac accagaggac
    41221 tgcgccggcg tgatctgatt ggaaagagac gtcgacgagg agggaaccgt cctaacgtgg
    41281 cgtcaggcct gctccgtcac gaacgggagc gcaagcatct gatttgctta tgaattatgc
    41341 cgtcctgacg tctttgtctt gttcaaagat tcggataggg ggatgtttcc caagaatggc
    41401 atcttcagtg atgaggactt ccttgatctg tttctgcgaa ggagcatcat acatgacgtc
    41461 caacatgact tcttcgagaa tggcacggag acctctggcc cctgttttct gaatgaaggc
    41521 tttccgggcg acggctgcca gcgctccttc ggtgaatttg agcttcacct tttcgaacga
    41581 aagaagtttt tcgtactgct tggtcaacgc gtttctcggt tcggtgagaa tcctgatcaa
    41641 ggcgcgttcg tccagttcgt ccaaggtggc gaccactggc aatcgaccga cgaactccgg
    41701 aatcagcccg tacttgagaa agtcttccgg ttgcactttt tcgaagagct cacccaatct
    41761 gacgtcgctt cgaccgcgca cttcggcgcc aaatcccatc gacttgcgat tcaaccgttg
    41821 ttcgatgatc tgctccaatc cgacgaatgc gccaccacag acgaacagaa tgttgctcgt
    41881 gttgacttga ataaactctt gatggggatg tttgcgtccg ccttgcgggg ggacattggc
    41941 cacggtacct tcaatcaatt tcagcagggc ctgctgcacg ccttcgccgg atacgtcgcg
    42001 cgtaatcgaa gggctgtcgc ttttgcgact gatcttgtcg atttcatcga tatagacgat
    42061 gccccgctca gcgcgttcga cgtcatagtc cgaggcctga aggagcttga ggatgatatt
    42121 ctcaacatcc tcgccgacat acccggcctc ggtgagagtt gtggcatcag cgagcgtaaa
    42181 cggaacatcc agatacttcg ccagtgtctg ggccaggagc gtcttcccgg tccctgtcgg
    42241 gccgaccatg agaatgttgc ccttctggag ctccacatcg tcgacgtctt tttctttggc
    42301 ggagatacgt ttgtaatggt tgtggacggc gacggagaga atcttcttgg cccgttcctg
    42361 cccgacgaca tactgatcaa gatggtgttt gatctccgag ggcttcttca gtttggagga
    42421 aatttcctcc ttcgcttctt cccaatcctc ggcgatgatg tcgttgcaca gattgacgca
    42481 ttcgtcgcag atatagacgg tggggccggc aatcagcttg cggacctcgt cccgactctt
    42541 cccgcagaac gaacagcgga gatggcgatc catcttttcc tgcttggcca tgcgccctcc
    42601 tgtcttactt gtccttggcc ccgtcttttc cgctctcgga tccccctacg gctttcagga
    42661 gtttcggggg ccgcgtaatc acttcatcga tcagtccgta ccgtttggct tcttcaccgg
    42721 acatgaaata gtcccgctcg gtatcatggg agattttttc gatcggctgg cccgtatgct
    42781 tcgccatgat ctcgttgaga cgctcgcgaa tcttcagaat ttccttggcg tgaatgtcga
    42841 tctcggtcgc ctgtccctgg aagcccccca gcggctgatg gatcatgacg cgggcattgg
    42901 gcaacgcgaa gcgtttgcct ttggttcccg ccgttaacag cagcgcaccc atgctggcag
    42961 cctggcccag gcagatcgta ttgatcggcg gcttcacgta ctgcatcgtg tcgtagatgc
    43021 cgaggccggc cgtgacgctc ccgccgggtg agttgacgta gagattgatg tctttc
//
LOCUS       DATIFR010000239        14882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1892860, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000239 DATIFR010000000
VERSION     DATIFR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892860"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VLD60_13850"
     CDS             complement(<1..760)
                     /locus_tag="VLD60_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB46093.1"
                     /translation="MQRRVMRMLIHAAVLGSVLSSSALAFPADWGKPLGAAYDGPEGK
                     PRPITPPPPELGSDEQTTMAVFERATKSVVFIANTAIQRDILSFDIMEVPRGSGSGFL
                     WNKQGHIVTNFHVIYGADAIKVTLADRSEHQAKLVGADPDHDLAVLQIQALETVLEPL
                     AVGTSHDLKVGQKVLAIGNPFGLDHTLTTGVVSALGRTIRSLTNRTIEGVIQTDAAIN
                     PGNSGGPLLDSAGRLIGVNTQIVSPSGAYAGIGFA"
     gene            complement(765..1187)
                     /locus_tag="VLD60_13855"
     CDS             complement(765..1187)
                     /locus_tag="VLD60_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSB46094.1"
                     /translation="MAVKTVPLLVEMRGSTQIENVTKAVQEALAGSRLAAGIVTVFVK
                     HTTASLMVIEDEPGIRADTKSWWDRTVPADPNWQHNRRNPGEDNGHSHLRGQLQGPSV
                     TIPFSNGAMLLGTWQQIVLVDFDTRARTRELIIQILGE"
     gene            complement(1189..2058)
                     /locus_tag="VLD60_13860"
     CDS             complement(1189..2058)
                     /locus_tag="VLD60_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46095.1"
                     /translation="MTHTTSLRAPLVVPRALATQLMRLYDYPHPVKRGHVIRGYDRAH
                     AIRTARMCAAVAASLGHDAERVRNYQIACLLHDLGRAGLDRPLFGKIWSWAKARGIPT
                     RPREWRAIYPETTYGRETEAFLSCYRKDLEADGVVMTPWAKEQVEMRLGYARRLARRL
                     RSGRTAIKKMGVRWEPWMQRVMLYYYYPEKLDGAKPWVKQLAEILVACEQFEAYSNQR
                     RGRDYYVRKKETIVEAFAYLEKLEQEGILSRDVIRALKRLAAEGAFDGIIAEARGHRL
                     TSSEKTLLRQMEH"
     gene            2207..2836
                     /locus_tag="VLD60_13865"
     CDS             2207..2836
                     /locus_tag="VLD60_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46096.1"
                     /translation="MPHATLKCSCCLAGTIIAGMLLGVATESVAAPLDQLTDLTGRAN
                     AIVTLTGRDNFTSEYRYNVSVRNLSGDPLIADSLIIVLDKITNLAGEDRENLQGESFL
                     TRFDVLDQDGMTDDGKPFFQIPAGNSPDLAPLTDSLPASVRIRNKDYVAVFTPAFRVY
                     GKKRLPAEPKQPKVQTTPGQPHNQTDKLIQLLLKKGVITEEEWRKATQP"
     gene            2833..3261
                     /locus_tag="VLD60_13870"
     CDS             2833..3261
                     /locus_tag="VLD60_13870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSB46097.1"
                     /translation="MSATVCKACSGHWPRTDHFIADLGLSMAYLHEDQFFPGWTVVVF
                     KRHATELFHLAPTERIQLMEEVNRAAKVLAETFEANKINYELLGNQLPHIHWHVIPRL
                     ASDPAPLEPVWRVQHEPVQSPASELQERIRRISGGIAKLR"
     gene            3386..4624
                     /locus_tag="VLD60_13875"
     CDS             3386..4624
                     /locus_tag="VLD60_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB46098.1"
                     /translation="MSRRLLLVTILLTALISQPPVHPSFAQSPDDQTPFAPPSESPPP
                     SIDESHPSEFTSRELEDAATMLANLRGNVRLFPDHSEARLKLAQGLYRIGDLDAAIDE
                     CRVAIKLNPNDAKAHLQLGVILMAKQDWRAAASVLKEAVRLDPAMTHAHYSLGSVQYS
                     LGNVKAAIQSYRQALELQPHFPDARYRLALLLKLTNRDQEAVQLMEDAAQGGISQAQF
                     FLGNAYKSGQGVEKDLSLAIFWWTTAVAFGHQPAADALSKLRRQALSSDHKDRRRQDA
                     LGAFHAYRDKLWKGFPNYSRTDDSETLGTRLLKENRADYAVSVLLQESYALSDVAQLE
                     LAKLYETGWGQHLAPFDKKILTCFETTAGEGFVPAKTILARIYGKGIGMPQDLPKAKS
                     ALKGLSKLEMKPVLDELGLQ"
     gene            4629..7292
                     /locus_tag="VLD60_13880"
     CDS             4629..7292
                     /locus_tag="VLD60_13880"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB46099.1"
                     /translation="MLDWPKLRRLFITSLSLQALTIGLLAALCAKAVWTVAPATFTAF
                     DWTAYDTWLRHRAPTAVSPSLTIVARDPASEERFGTLLDRALLAQVITVAHEAGAAAI
                     GVDHRLDHASPAQLGGAASDALLIEAVHTAAPVVFVHDPDSILRSDAAILGHVVVSTD
                     PDHVARQIPLLTMVEAQTVPAFGSILYDLSQQQASPATKSMVTERAAPTLVNPVGNGS
                     LAVLPTIPLSSVWEAIQHHDDEKLDGWFKGKVVVILPNPMTQSPWLLPTGQSVSGMVA
                     HIHLLNSLLTDNRLCQVGSVGRYVTTVLLGSIVTWCLLQFRGSISLLFAGCAIAIYGA
                     LMMTMLVGLHLVLPVALPLTAALFVLLSTTAWTHLTTGQRMVLLERDMLRVQQDAAAV
                     REALILRENRAEALQEDLEAARSAAAQSAGQQEEQARTTEALRSQIAETQGQEQTARQ
                     QLEQLERQLHDLRAASTEAGAVGDALLDQLRNECRQLGIITQDPTLLRMFRDVKKGAK
                     SPLTVLLLGEPGTGKELFAKAVHRLSSRGSKTFIAVNMAAISPELFESELFGHMKGSF
                     TGATADRRGYFELANHGTIFLDEIGDLRLDHQSKLLRVLQEQSFYRVGATTPTTVDVR
                     IVAATNRDLQRGVSEGWFREDLYFRLKGLVFRLPPLRERAHDIPIIAEACLAEIATQM
                     GRAVPKLSNDALRVLTEHDWPGNVRELRHALERAVALSDGPVLTKNSFYSEAVSTNEE
                     QRATPAAALPDSASDAAVLTCLRQHGFDMQVTAKTLGWDRSTVTQRLKGLCFQALVES
                     NGDQAKAAAAIAGDPSHLRTVELKLRDYHGHLMSVIEPFGTADEALHDCKRRFKNLPD
                     RHFVSVAHLVRQYFRQNSDLSPSRTTTLKPS"
     gene            complement(7294..8499)
                     /locus_tag="VLD60_13885"
     CDS             complement(7294..8499)
                     /locus_tag="VLD60_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB46100.1"
                     /translation="MEPIFLTATLWLALAVVSALIASSLGLSIALVEICVGVGAAATT
                     GWFGLGDVLAANSEWVRFLAASGAVVLTFLAGAELDPDVVRTKLTEVTVVGLVGFFAP
                     FLGCAAVAYYVLGWDTNASWLCGIALSTTSMAVVYAVMLETGFNKTGFGKGILAACFI
                     NDLGTVIALGLLFAPFTYKTIVFIVGSLMALALLPLTTAWLTRHYAYRTAAIRTKWVM
                     LILFGLGALALWSGSEAVLPAYLAGMVLAGAAARDAHWMRRLRTLTVGFLTPFYFLRA
                     GTLVSLPALLAAPLVFLALLMGKVAAKIFGLYPFISAFRKERNERWYYTLLMSTGLTF
                     GTISSLYGLAHGLVTQEQYSFLVAAVIASAVVPTLIAGLFFVPTHLLPKPDHAEKAVR
                     RINGLSDEG"
     regulatory      complement(8589..8653)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(8728..9279)
                     /locus_tag="VLD60_13890"
     CDS             complement(8728..9279)
                     /locus_tag="VLD60_13890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB46101.1"
                     /translation="MNLTFPIEAKLKDCSPVQLVLADQQDVEPLRDLYRFIVEEGTSY
                     PHDRLPNQDDFMDYWFRGRSTVAAYVPDRARAASMAGAFYLKPNWPGRARQVANAGFI
                     VAPDWRNKGLGWLLGATMLAYAKQLGYRSVIFNLVFSENVIARRLWEKLGFKELGVIP
                     GAVRRNNGTYQDAVIMFRSLLEN"
     gene            complement(9366..9803)
                     /locus_tag="VLD60_13895"
     CDS             complement(9366..9803)
                     /locus_tag="VLD60_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46102.1"
                     /translation="MASDAAKETTRRAWRELGFFCGRDKVAKEWRIVGSVKGLKKFAS
                     EIRKYASNPAHDRLSEYMHLGPAMNLEICTSHQTEITDQWIGAPVTDFHHLAALVDRS
                     VQANMVGKRIALRANFSPMAPYELILDVREDAFDPASADPTCQ"
     gene            10575..11390
                     /locus_tag="VLD60_13900"
     CDS             10575..11390
                     /locus_tag="VLD60_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB46103.1"
                     /translation="MQKGPSQVTSGNIAPTISGPRKDKDVFVVSGLVISLAAIIGAFW
                     YYSSTDQMTTTSPDRLTESQVSEVLKSTSQPAATPVSLPMNKIQEAPVSTAKPGIIHA
                     DIYFEVGRKGLTDEAKKLLQEQAVLLKNDGNLGVLVQGYTDAQGSASYNMKLGLKRAD
                     SVKAELMNAGVAEHQIKVVSLGKDGVLCIDNSDVCRQMNRRVHLEIRTIGQEHMVAPV
                     VATTPAVDATQAAIDPSQQADDSNSLIDRLIPSASASQAEDPTATPLEPASGS"
     gene            11486..13600
                     /locus_tag="VLD60_13905"
     CDS             11486..13600
                     /locus_tag="VLD60_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HSB46104.1"
                     /translation="MRIQTTRGLIIRRTAHLLLITIIMTFLFWAGGLPVLLACGEEPV
                     PRDVRMTLKDVTQGSLLFKTNEVGRYLPAPILKTDVQISVTGIIARTALKQEFINPSR
                     RKDDWAEGIYVFPLPETAAVDHLRMKVGDRTIEGQIKERAEAKKVYEQAKQEGKRASL
                     VEQERPNIFTTSVANIAPGDRITVEIEYQETVRYDQGRFSLRFPMVVGPRYIPGTPVV
                     MEDQPPGHGWSLDTDRVTDASRITPPVQHPNRGPINPVSLTIDLAAGFPPGKIESPYH
                     EILTITEPDGRQHITLREDRVPANKDFQLTWQPAPGQAPIATVYRQQQGDSSYTLLMV
                     TPPSSYQWVAQVPRETIFVIDTSGSMAGTSIEQARAALLLALNRLTAQDRFNIIQFNS
                     VTHILFSQPQPVRTDTLRKAVHYVEQLRANGGTEILPALKIALKGAAPSTHLRQVIFL
                     TDGQVGNEDELFGIIREQLQASRLFTIGIGSAPNSHFMRKAAEFGRGTFTHIGSTNEV
                     KKQMDAIFQKLERPVLTDLHVEGLDGAAELFPPRIPDLYDGEPVVVSLKIQTTPINVT
                     IKGSIGSNPWTTTVNLTDRPERDGLSIYWARQKIASLMDQQRHGQDDSATRQSVLDVA
                     LAHHLVSRYTSLVAVDVEPIRPTDKSLVTHAMKVNLPEGQDYQAIFGLPKTATSGQLQ
                     ILLGLAALTMAWLVRAHRKLVA"
     gene            13687..14394
                     /locus_tag="VLD60_13910"
     CDS             13687..14394
                     /locus_tag="VLD60_13910"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HSB46105.1"
                     /translation="MATDRVGVVCSLFKKAVQQGRSKRRGEAYSVSYVEPLRVARTPL
                     AAFFKRLLTGCILAIGLWQIGEGSWIYAKARLAQYLLQRAWSRTLAGETTAKPWPWAD
                     TWPIARLIVPSRGIDLIVLNGAYGRTLAFGPGYVESSAFPGSFGTTILTGHRDTHFRF
                     LKTLRKDEPILIQSRTGTWHRYRMRERQIVNADTAVIRQDDDARQLVLVTCYPFDAIV
                     PGGPLRYVLTADLEDAE"
     gene            complement(14410..>14882)
                     /locus_tag="VLD60_13915"
     CDS             complement(14410..>14882)
                     /locus_tag="VLD60_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HSB46106.1"
                     /translation="VTGLLLAVAVIGPDVSWADFHASVVTVHEGDRVTIHHNGRSEMI
                     YLKNIDCPELKQPYGKQATRTTLAYIGGREVVIHGLQRDRQGRTTADVLLLDGRNVGH
                     ELVKEGLAWARLETAEGRRLGEIEQLARAEHKGLWSDANPVPPWKWKQKKKVKR"
BASE COUNT         3258 a         4592 c         4099 g         2933 t
ORIGIN      
        1 cggcaaatcc aataccggca taggcgccac tggggctcac gatttgcgtg ttcacgccga
       61 tcaaacgtcc ggcgctgtcg agcaacggtc cgccgctgtt gccgggattg atggccgcat
      121 cggtttgaat cacaccctcg atggttcgat tcgtcaacga cctgatcgtc cgccccagag
      181 cgcttacgac accggtcgtc aacgtatgat ccagtccgaa cggattcccg atcgccaaga
      241 ccttttggcc gaccttcaaa tcatgcgagg tgcccacggc cagcggctcg aggacggtct
      301 cgagggcctg aatctgcaac accgccaaat cgtggtccgg atcggcgccg acgagcttgg
      361 cctgatgctc gctgcggtcg gccagcgtca ccttgatggc atcggctccg taaatgacat
      421 gaaaattcgt gacgatatgc ccctgtttgt tccacaagaa ccctgagcct gaaccccgcg
      481 gcacctccat gatgtcgaag gacaagatgt cacgttgaat cgccgtattg gcgatgaaca
      541 ccacggactt cgtggcccgt tcgaagaccg ccatcgtggt ctgctcatcc gatcccaact
      601 ccggaggtgg aggggtgatc gggcgcggtt taccttccgg tccatcgtag gcggccccaa
      661 gcggcttacc ccagtcggcg ggaaaggcca gcgcactcga tgacagcaca ctgcccagca
      721 ctgcggcgtg aatgagcatc cgcatgacgc gccgctgcat ggaattactc tccgagaatt
      781 tgaatgatta gctcgcgggt gcgggcgcga gtatcgaaat cgacgaggac gatctgctgc
      841 caggtgccca acagcattgc accgttcgag aagggaatgg tgaccgatgg cccttggagc
      901 tgaccgcgaa gatggctgtg cccgttatct tcaccgggat tgcgccggtt gtgctgccag
      961 ttcggatcgg ccggcaccgt tcggtcccac caggatttcg tatcggctct gatgcccggc
     1021 tcgtcttcaa tcaccatgag agaggcggtc gtatgcttca cgaagaccgt cacgatgccg
     1081 gccgccagcc tcgaaccggc cagcgcctcc tgcaccgctt tggtcacatt ctcgatctgc
     1141 gtgctgccac gcatctcgac cagcaaggga acggtcttca cagccatctc aatgctccat
     1201 ctggcgcagc aacgtcttct cgctcgacgt caggcgatgt ccacgcgctt ccgcaatgat
     1261 gccatcgaag gcgccctcgg cagcgaggcg ttttaaggca cgaatgacat cgcggctgag
     1321 aataccttcc tgctccagct tttcaagata cgcgaaagcc tcgacgatcg tttccttctt
     1381 ccggacataa taatcacgcc cacgccgttg attgctgtaa gcctcgaact gctcgcaggc
     1441 tacaagaatc tccgcgagct gcttgaccca cggcttcgct ccgtccaatt tttccggata
     1501 gtagtaatag agcatcaccc gctgcatcca gggctcccat cgcacgccca tcttcttgat
     1561 ggctgtccga cctgatcgca accgcctggc caaccggcgc gcgtagccca accgcatctc
     1621 gacctgttcc ttcgcccagg gcgtcatcac gaccccgtcg gcctccagat ctttccggta
     1681 acagctgaga aaggcttccg tttcgcggcc ataggtcgtt tccggatata tagctcgcca
     1741 ttcacggggt ctggttggta tcccgcgggc cttcgcccag gaccaaatct tgccgaagag
     1801 tggccggtcg agaccggcac ggcccaggtc atgcagaaga caagcaatct gatagttgcg
     1861 cacgcgttcg gcatcgtgcc cgagtgatgc cgccacggcc gcgcacatgc gtgcggtccg
     1921 gatggcatgg gcccgatcgt acccgcgaat gacatgacca cgtttcacag gatgaggata
     1981 gtcgtacaaa cgcatgagtt gagtggccaa tgctcgggga accacgagcg gagcgcggag
     2041 acttgtcgtg tgtgtcatag ggccgggagc acaattgtcg aggagaatat agttcgtcga
     2101 acggaagtgt caaggcaggc ccatggctcg gggctcgatc aattcaacta tctttgatat
     2161 gctccgtgag gccggccaat ggcccttgtc cgagatgagg aataccatgc cccatgcaac
     2221 attgaaatgc tcctgttgtc ttgctggcac cataatcgcc ggcatgctgc tcggcgtcgc
     2281 cactgaatcg gtcgcggctc cacttgatca actcaccgat ctcaccggac gtgccaacgc
     2341 gatcgtcacg ctcacgggcc gtgataactt tacaagcgaa tatcgctaca atgtcagcgt
     2401 ccgcaatctg tcgggcgatc cgctcatcgc tgactcactc atcattgtcc tcgacaagat
     2461 cacgaatctg gcgggagaag accgtgagaa tttgcaaggt gagtcatttc tcactcgatt
     2521 cgacgtcctc gaccaagatg ggatgaccga cgacggaaaa cctttttttc agattccggc
     2581 cgggaatagc cccgatctcg ctccgcttac cgacagcctc cctgcatccg tccgcattcg
     2641 caacaaggac tatgtggccg tctttacccc tgccttccgt gtctacggga aaaaacggct
     2701 tccggctgaa cctaaacagc ccaaagtgca gaccacacca ggccaacccc ataatcaaac
     2761 ggacaagctg atccagttgc tcctgaagaa aggtgtgatc accgaagagg agtggagaaa
     2821 agcaacccag ccatgagcgc caccgtctgc aaggcctgtt caggccactg gcctcgcacc
     2881 gatcacttca ttgccgatct gggcctctcg atggcctatc tccatgagga tcaatttttc
     2941 cccggttgga cggtggtcgt gttcaaacga cacgcgacgg aactgtttca cctcgcgccg
     3001 accgagcgga ttcaactcat ggaggaagtg aaccgggccg caaaggttct tgcagaaact
     3061 ttcgaggcga acaagatcaa ctacgaactg ctgggcaacc agctgcccca tatccattgg
     3121 cacgtgattc cacgccttgc cagcgatccg gcccccctgg aaccggtttg gcgagtccag
     3181 cacgaaccgg tccaatcacc ggcctcagag cttcaggagc gtattaggcg catcagcggt
     3241 ggaattgcca agcttcgata gcgaacctcc tcccggcccc cctctacacc aagatcacgc
     3301 tgttgcaagt cggtctgaat agccccacca ggtgctataa tggtgtccac tacgcggata
     3361 gcgtgaggga gggtcacagg gttcgatgag ccgtcgatta ctgctggtga ccatcctgct
     3421 cacggctctc atctcgcaac cacccgttca tccgtcattc gcgcagtctc ctgacgatca
     3481 gacacctttt gctcctccca gtgagtctcc tccgccttcg atagacgaat ctcacccatc
     3541 tgaattcacg tcacgcgagt tggaagatgc cgccaccatg ctggccaact tacgcggcaa
     3601 cgtcagactg tttccggacc actcggaggc gcgactcaaa ttggcgcaag gactctatcg
     3661 gatcggcgat ctcgacgcgg caatcgacga atgccgcgtt gccatcaaat taaatccgaa
     3721 cgacgccaaa gcccatctgc aactcggcgt catcctgatg gcgaaacagg actggcgcgc
     3781 tgcagcctcc gtattaaaag aagccgtccg gctcgatccc gcaatgaccc atgcccatta
     3841 cagcctcggc agcgtgcagt actcattagg caatgtgaaa gcggctattc aatcgtatcg
     3901 gcaagcgctc gaattacaac cgcacttccc cgacgcccgt tatcgcctcg cgctgttgtt
     3961 gaaactgacg aatcgcgatc aggaagcggt ccaactcatg gaagacgcgg cgcaaggggg
     4021 catctcgcaa gcgcagttct ttctcggcaa tgcctataag agcggacaag gtgtcgagaa
     4081 agatctgagc ctggcaatct tctggtggac gacagcggtt gcattcggtc atcaaccggc
     4141 ggcagacgcc ctttccaagc tccggcgcca ggccctctcg tccgatcaca aggatcggcg
     4201 gcgccaagac gcgctcggcg cctttcacgc ctaccgcgac aagctctgga aaggattccc
     4261 gaactatagc cggactgacg acagtgagac gctcggaacg agactgctga aagagaatcg
     4321 agccgactat gcggtctccg tcctactgca agaaagttac gcgttgagcg atgtggcgca
     4381 gcttgaatta gcaaagcttt atgaaacggg ctggggccag cacctcgcac cattcgacaa
     4441 gaagatcctg acctgtttcg agaccacggc cggcgaaggt tttgtgccag ccaaaacaat
     4501 actggcgcgt atctacggca aaggcatcgg catgccacag gatctcccaa aagcgaaatc
     4561 cgcgctcaaa gggctgtcga agctggagat gaaacctgtg ctcgacgagc tgggcctcca
     4621 ataattccat gcttgattgg ccaaaactca ggcgcctctt cattacctcg ctttcactgc
     4681 aagcgctgac gatcggactg ctcgcagccc tgtgtgccaa agctgtatgg accgtcgctc
     4741 ccgccacgtt caccgcgttc gattggacag cctacgatac ctggctccgc catcgcgcgc
     4801 cgacagccgt cagcccatca ctaacgatcg tggcgcgcga cccagccagc gaggagcggt
     4861 ttggaactct gttggatcga gccctgctcg cgcaggtgat taccgtcgcc catgaagccg
     4921 gtgccgctgc gatcggtgtc gaccatcgcc tcgatcacgc cagtccggca cagctcggcg
     4981 gagcagccag cgacgccttg ctgatagagg cagtccatac agccgcgccg gtggtctttg
     5041 tccacgatcc cgattcgatc ctgcgatcag atgccgcaat cctggggcat gtggtggtct
     5101 caaccgatcc tgaccacgtc gcgcgccaga tccctttgct gacgatggtc gaagcccaga
     5161 ccgttccggc cttcggctcg atcctgtacg acctctctca acaacaagca tccccggcaa
     5221 ccaaatcaat ggtcacagaa cgggcggcac cgacgctcgt caaccccgtc ggcaatggct
     5281 ccctggcagt attgccgacg attccgttgt cctctgtctg ggaagccatt cagcatcacg
     5341 acgacgagaa gctcgatgga tggttcaaag gtaaggtggt tgttatcctt cccaatccca
     5401 tgacgcaaag cccctggctt ctgccgaccg gtcaatcggt cagcggcatg gtcgcgcaca
     5461 ttcacctgct caacagcttg ctgaccgaca accggctctg tcaggtcggt tcagtcggac
     5521 gctacgtcac cactgtcctc ttgggttcga tcgtgacctg gtgcctcttg cagtttcgcg
     5581 gatcaatcag cctgctgttc gccggttgtg ccatcgcaat ctacggcgcg ctgatgatga
     5641 ccatgctggt tggacttcac ctcgtactgc cggttgcatt gccgttgacc gctgcgctgt
     5701 tcgtgctcct cagcacaacg gcctggactc atctgacaac cggccagcgc atggtcctgc
     5761 ttgaaaggga catgttgcgt gtacagcaag acgcggccgc agtcagggaa gcgctcatcc
     5821 tgcgggagaa tcgtgcggag gcacttcagg aagatctgga agcagccaga tcggctgcgg
     5881 ctcagtcagc aggccagcag gaagaacagg ccagaacgac cgaggcgttg cgatcgcaaa
     5941 tcgccgagac acaaggccag gaacagacgg cccggcagca gctcgaacaa ctcgagcggc
     6001 aactccacga cctccgcgcc gccagcacgg aagccggcgc agtcggagac gctctactcg
     6061 atcagctccg aaacgaatgc cgccagctcg gcatcatcac gcaagatcct acgcttctac
     6121 ggatgttccg tgacgtgaag aaaggcgcca agtcgccgct gaccgttctg ctcctcggtg
     6181 aaccaggaac cggcaaagag ctcttcgcca aggctgtgca tcggctcagc tcaagaggga
     6241 gcaagacctt tatcgcagtg aacatggcgg cgatctcgcc ggagctgttc gaaagcgaat
     6301 tattcggcca tatgaaaggc agcttcaccg gagccacagc agaccggcgc ggctatttcg
     6361 aactcgccaa tcacgggacc attttcctgg acgaaatcgg cgatcttcgg ctggatcacc
     6421 aaagcaaatt actgagggta cttcaagaac agtcgttcta tcgcgtcggc gcgacaaccc
     6481 cgacgacggt ggacgtgcgg atcgtcgccg caaccaatcg cgacttgcag cgtggcgtct
     6541 ccgaaggctg gtttcgcgag gatctctatt tcaggttgaa gggtttggtc ttccggctcc
     6601 cgccgctgcg agagcgagct cacgatattc cgatcatagc ggaagcctgt ttggctgaga
     6661 ttgccacgca gatgggacga gccgttccca aactgtcgaa cgacgcgctt cgtgtcctga
     6721 ccgagcacga ctggccgggc aacgtgcgag agctgcgtca cgccctcgag cgggccgtcg
     6781 ccttgagcga tggccccgta ctcacaaaga actcgttcta ttctgaggca gtctcaacga
     6841 atgaggaaca gagagcgacc ccagctgcgg ctctaccgga ttcagccagc gacgcagccg
     6901 tgttgacttg cttgcggcaa catgggttcg acatgcaggt gacggccaag acgttaggat
     6961 gggaccgcag caccgtcacg caacgattga aggggctctg cttccaagcg ctggtcgaat
     7021 ccaacggcga ccaggcaaaa gcggcggcgg cgatcgccgg cgacccttcc cacttgagaa
     7081 ccgtcgaact caagctcagg gactatcacg gccatctcat gtccgtgatc gaaccgtttg
     7141 gtacggccga cgaagccctc cacgactgca aacggcgctt caagaacctt ccggatcgcc
     7201 acttcgtttc ggtcgcacat ctcgttcggc aatacttccg tcaaaactca gatctgagtc
     7261 caagcagaac aaccactctc aaaccttcct gacctagccc tcatcgctca ggccattgat
     7321 tcgcctgact gctttctccg cgtgatcagg tttcggcaag aggtgggtcg gaacaaaaaa
     7381 cagacccgca atgagtgtcg gtaccaccgc gctcgcgatc accgccgcca cgagaaagga
     7441 atattgttcc tgggtcacca acccatgagc caggccatag agagaagaga tggtcccgaa
     7501 cgtcaagccg gtcgacatca gaagcgtgta gtaccaacgt tcattgcgtt ccttccggaa
     7561 ggcgctgata aagggataga ggccaaaaat cttggccgcg accttgccca tcagcaacgc
     7621 gagaaagacc aacggcgccg cgagcaaggc cggcagagat acgagggttc cggctcgaag
     7681 aaagtaaaag ggggtgagaa accccacggt cagcgttcgc agccgtcgca tccaatgcgc
     7741 gtccctcgca gcggctcccg cgagtaccat gccggcaaga taggccggaa gaaccgcttc
     7801 gctccccgac cataaggcga gtgcgcccag cccgaacagg atcagcatga cccatttcgt
     7861 tctgattgca gccgtacgat aggcatagtg cctggtcagc cacgcagtcg tgagcggcaa
     7921 caaggccagt gccatcaggc taccgacgat aaacacaatg gtcttatagg taaagggtgc
     7981 gaagagcagg cccagcgcga ttaccgttcc gagatcattg ataaagcatg ccgcaagaat
     8041 ccccttcccg aaccccgtct tattgaagcc tgtttccagc atgacggcat agaccaccgc
     8101 catagaagtc gtcgagagag caatgccgca gagccaactc gcattcgtgt cccatcccaa
     8161 tacgtagtag gccacggcag cacagccgag aaacggcgca aagaatccga cgagtccgac
     8221 gacggtcacc tcagtcagtt tcgtccgtac gacgtcggga tcgagctccg cccctgccag
     8281 aaaagtcaga actaccgcgc cggatgccgc gagaaatcgt acccactccg agttggcggc
     8341 gagcacatcg ccaaggccga accagcccgt ggtggcagca gcaccgacgc ccacacaaat
     8401 ttcaacaagc gcaatcgaga gaccgaggga agaggcaatg agggcggaaa caaccgcgag
     8461 ggcgagccag agggtggccg tgagaaagat cggttccatc gaggagtcct ccgttccggc
     8521 cagggttgca gactgcttcg gaccggttgc cgccacgctg gcgacaatcc tacggctacg
     8581 gcagtaaaag tagcgtagga gtcatcagcc tggccgaggc ggttgtacgg gggaccccat
     8641 cccctgtaga tgcgcagagt atacgcgaaa tctagaggga gtgttacaga ttaatgatgc
     8701 gtctgagata ttggcggcca cttccggtca gttctcaagc aacgatcgaa acatgatcac
     8761 cgcatcctga taagtgccgt tgttcctccg caccgcgccg gggattaccc ccaattcttt
     8821 gaagccaagt ttttcccaga gacggcgtgc gatcacattt tcagagaaca caaggttgaa
     8881 gattacgctg cggtagccga gttgcttggc ataggccagc atggtagcgc ctaacaacca
     8941 gcccaaacct ttgttgcgcc agtccggtgc gacgatgaag ccggcgttcg cgacttgcct
     9001 tgcccgcccg ggccagttcg gcttgagata gaaagctccg gccatgctcg ccgcacgggc
     9061 acggtccggc acgtaagccg ccactgtgct cctgccgcga aaccaatagt ccatgaagtc
     9121 atcctgattc ggaagccggt catgcgggta cgaggtcccc tcttcgacaa tgaatcgata
     9181 gaggtcccgc aatggctcga catcctgctg atcggccagc accaattgaa ccggcgagca
     9241 gtccttgagc ttcgcctcga tgggaaatgt gaggttcaat tgactcgacc tattagcttt
     9301 ttcttgtccc gtaggattgg gaaaagattc ctcacacctt taccttcacc ttacccgtct
     9361 caccttcact ggcaagttgg gtcggcggac gcaggatcaa aggcatcctc gcgaacgtcc
     9421 aagatgagtt cataaggagc catcggcgag aaattcgctc gcaatgcaat gcgctttccg
     9481 accatgttgg cctgcacgct ccgatcaacc aacgctgcga gatggtggaa atccgtcacg
     9541 ggagcgccga tccactggtc ggtaatttcc gtttgatgcg aggtgcagat ttccagattc
     9601 attgccggtc ccaggtgcat atattccgac agccgatcgt gcgccgggtt ggaggcgtac
     9661 ttccgtatct cggaggcgaa tttcttcaag cccttcaccg acccgacaat gcgccactct
     9721 ttcgcgacct tgtcccgacc gcagaaaaac cccagctcac gccacgcccg ccgcgtggtt
     9781 tccttcgctg cgtcactcgc cattacgtca ctcctctagc aggatgctga aaaactccgc
     9841 cagcttcgtt ctcgcatcgt tcagaccctc aatgtacccc agggggtacg actcggcccc
     9901 tcactcgctg cggccttgcc ggacgaactt tttgagcatc ctgcgaactc accgcaccat
     9961 actcctccaa catcaaacct ctactcgctt atgccacggc tttaccccac tttgcacaac
    10021 atagcaattc ttaccaaaca ctctgctggg gcaagtctac ggcaaagccc aacaggtatc
    10081 caccgccgcg ctcttgggtg gtcaggatct tcaaactcgc cgtcccgcaa ggccgcaggt
    10141 tagatatagc tccctccaag ctcgctcgtc tatgtcttca gggggcagcc tggttggtcc
    10201 tcgattgcgc gcgtcgaacg agcaccaccg gtaatttacc aatcggatag atcttgcttg
    10261 ccgcgttccg cgagcaggag gacgaccagg ctgccctatc tcctcccttg cgggtccatc
    10321 cccgcatccc ttgatgggga aggcgtccac gcattcttgt ctagtgcact tcgctctctg
    10381 aacccctgat tattcttcac atctctctcg acttcgcccc ttcgcgttat cggcattccg
    10441 gttgctcaag cacaacagcg tcggcgcaca ggaaagcagg ttgggaaggg ggtgattcgg
    10501 ggccaaggcc gatttgtacc tgtcagcttg tcagtctgaa acttttacac ttttaacttt
    10561 aggaggattc catcatgcag aaaggtccat cacaggtgac gtccggcaac atcgccccga
    10621 ccatttcggg acccagaaaa gataaagacg tgtttgtcgt ctccgggctg gtgatcagcc
    10681 tggccgccat tatcggcgcc ttctggtact actcttcaac cgatcagatg accaccacca
    10741 gtccggatcg cctgaccgaa tcgcaagtca gcgaagtcct caagagcacc agccaaccag
    10801 cggccactcc tgtttcgctc ccgatgaaca agatccaaga ggctccggtc tccacggcca
    10861 agccggggat catccacgca gacatttatt tcgaagtggg ccgcaagggt ttgaccgatg
    10921 aagccaagaa gttgcttcag gaacaggctg ttctcctcaa gaacgatggc aacctcggcg
    10981 tgttggtcca gggctacacc gatgcccagg gctccgcgag ctacaacatg aaactgggtc
    11041 tcaagcgggc cgatagcgtg aaagcagagt tgatgaacgc cggcgtggca gagcaccaga
    11101 tcaaggtcgt gagcctcggc aaagacggcg tgctctgcat cgacaacagc gatgtctgcc
    11161 gccagatgaa ccgccgggta cacctcgaaa tccgcacgat cgggcaggag cacatggtcg
    11221 caccggttgt ggcgacgaca ccagccgttg atgccaccca agcggctatc gatccgagcc
    11281 aacaggctga cgactccaat tccttgatcg acagactgat cccttcagcc agcgcctctc
    11341 aggctgaaga cccaaccgca acgcctctcg aaccggcctc cggtagctaa tgctggcctg
    11401 gatatgcgcc tgcacgaatc ccctcgtgcg ggcgcagcca tgcagagtca acgccaccac
    11461 ttaccccaca cgagacgagg acggcatgag gatacaaact acaagaggac tgataataag
    11521 gcgcacggcg catcttctcc tgatcaccat catcatgacc tttttattct gggccggcgg
    11581 gctgccggta ttgctcgcct gcggagaaga accggtccca cgtgatgtca gaatgaccct
    11641 gaaagatgtc acgcaaggca gcctgctctt caagaccaac gaagtgggcc gttacctccc
    11701 tgctcccatc ctcaagacgg acgtgcagat ttccgtcacc ggcatcatcg cccgcacggc
    11761 cttgaaacag gaattcatca atcccagccg tagaaaagac gactgggccg aaggcatcta
    11821 tgtcttcccc ttgccggaga ccgccgccgt cgaccatctg cgcatgaagg tcggcgaccg
    11881 gactattgaa gggcagatca aggaacgcgc cgaggcgaag aaggtctacg agcaggccaa
    11941 acaggaaggc aagcgggcaa gcctggttga acaggaacgg cccaacattt tcaccacatc
    12001 ggtcgccaat atcgcgcctg gcgaccggat cacggtcgag atcgagtatc aggaaacagt
    12061 tcgctacgac cagggccgct tctctctgcg gtttccgatg gtcgtcggcc cacgctacat
    12121 tccgggcacc ccggtggtga tggaagatca gccgcccggc cacggctggt cgctcgacac
    12181 ggatcgcgtg accgacgcct cccgcattac cccgccggtg cagcatccga atcgcggccc
    12241 gattaatccg gtcagcctga cgatcgatct ggctgcaggc tttccccccg gcaagattga
    12301 gtctccctat cacgagattc tcaccattac agagccggac ggacggcagc acatcacgtt
    12361 gcgcgaggat cgcgtgccgg ccaacaagga ttttcaacta acctggcagc ctgcgccggg
    12421 acaggctccg atcgcgaccg tctaccgaca gcagcagggc gacagctctt atactttgct
    12481 gatggtcaca ccgccttcat cttatcagtg ggtcgcgcag gttccacgtg aaacgatctt
    12541 cgtcatcgat acgtcgggct cgatggccgg cacgtcgatc gaacaggcca gggccgcctt
    12601 gctcctggcg ttgaaccgtc tgacggcgca ggaccggttc aatatcatcc agttcaacag
    12661 tgtcacgcac attctctttt cacagccgca accggtaaga acggacacgt tgcgcaaggc
    12721 ggtccactat gtcgagcaac tcagggccaa tggaggcacg gaaattcttc cggccttgaa
    12781 gatagccctc aagggtgcgg cgccctccac acacttgcgg caagtgatct tcctgacgga
    12841 cggccaggtg ggtaacgaag atgaactgtt cggcatcatt cgtgaacagt tgcaggcgag
    12901 ccggctgttt accatcggca tcggctcggc cccgaatagc cacttcatgc ggaaagcggc
    12961 ggagttcgga cgcggcacct tcacgcacat cggtagcacg aacgaagtga aaaaacagat
    13021 ggatgccatc ttccagaagt tggaacggcc ggtgttgacg gatctgcatg tcgaaggact
    13081 tgacggagca gccgagctat tcccgcccag gattccggat ctctacgacg gcgagccggt
    13141 agtcgtctcg ttgaaaatac agacgacccc gatcaacgtg acgatcaaag gctcgatcgg
    13201 ctcaaatccg tggacgacga cagtgaattt gacggaccgg cctgaacggg atggcctgtc
    13261 catctactgg gctcggcaga agatcgcctc gctgatggat cagcagcgcc atgggcagga
    13321 tgattccgca acgcggcagt cggtgctcga cgtggcactg gctcatcacc tggtcagcag
    13381 gtacacgagt ctggtagctg tcgatgtgga accgattcga ccaacggaca agtcgctggt
    13441 cacccatgcg atgaaggtaa atctgccgga aggtcaggat tatcaggcga tcttcggctt
    13501 gccgaagacc gcgacgagcg ggcaactaca aattttgttg gggttagctg cgctcacgat
    13561 ggcgtggctg gtacgggccc accggaaact tgtcgcatga acccgatccg ccctacttcc
    13621 cgtgctctcg ctctgctgat aggctttcga gatcaactga cacatcatca ccggaggcag
    13681 caatacatgg cgaccgatcg tgtcggtgtg gtatgcagcc tgttcaaaaa ggccgttcag
    13741 caaggccgca gcaagcgaag aggcgaggcg tactctgtgt cgtacgtgga gcctcttcgc
    13801 gtcgcgagaa cgccgctggc ggcctttttc aaaaggctcc tgacggggtg catcctcgcc
    13861 atcggactgt ggcagatcgg ggaaggatcg tggatctatg cgaaggccag gctcgcgcag
    13921 tacttacttc agcgggcctg gtcccgcacc ctcgcgggag aaaccacggc gaaaccctgg
    13981 ccctgggccg acacttggcc aatcgctcga ctcattgtgc caagtcgggg gatcgatctg
    14041 attgtcttga acggggccta cggccggacg ctggcctttg gccctggcta cgtggaatcg
    14101 agtgcgttcc ccggatcgtt cggcacgacg attctcaccg gtcatcgcga tacgcacttt
    14161 cgatttctga aaacactgcg gaaggacgag ccgattctga ttcagtctcg caccggaaca
    14221 tggcatcgat acaggatgcg cgagcgtcag atcgttaacg cggacaccgc agtaattcgt
    14281 caggacgatg atgcaagaca gcttgtgctc gtgacgtgct atcccttcga tgcaatcgta
    14341 ccgggaggac cgttgcgtta tgttctgact gctgatttgg aagatgcgga gtaaagagca
    14401 gtggcaggct cagcgcttaa cttttttctt ctgtttccat ttccacgggg gaaccggatt
    14461 ggcatctgac cagagacctt tgtgctcggc tcgcgcgagt tgttcgatct cgccaagccg
    14521 tcggccctcg gcagtttcca gtcttgccca ggcgaggcct tccttgacca gttcatgccc
    14581 gacattgcgt ccgtccagaa ggagcacatc agccgtcgtc cggccttgcc gatcgcgttg
    14641 cagtccgtgg attacgacct cgcgtccgcc aatataggct aacgtcgtcc gtgtcgcctg
    14701 tttcccataa ggctgtttga gctccgggca atcgatattt ttcagataaa tcatctcgct
    14761 acgcccgttg tggtggatcg tcacccgatc cccttcatgg acagttacca cgctcgcatg
    14821 aaagtcggcc catgagacgt cagggccaat gaccgccacg gccagcagca atccggttac
    14881 gg
//
LOCUS       DATIFR010000240         1258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_744283, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000240 DATIFR010000000
VERSION     DATIFR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744283"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1257)
                     /gene="tuf"
                     /locus_tag="VLD60_13920"
     CDS             complement(70..1257)
                     /gene="tuf"
                     /locus_tag="VLD60_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSB46107.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHIDHGKTTLTAAITKVLGKNNPKIQ
                     FRSFDSIDNAPEEKARGITIATAHVEYETKNRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAMVVFMNKVDAVDDPELLDLVELEVRELLT
                     SYGFPGDEIPIVRGSALQALNGEGDGEKGIEELMEAVDSYIPQPAREIDKPFLMPVED
                     IFSISGRGTVATGRIERGQVKVGEEVEIIGIKATRKSVVTGVEMFKKLLDSGMAGDNV
                     GLLLRGVERKEIERGQVIAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFSGYRPQFYF
                     RTTDVTGVATLNEGVEMVMPGDNSQLTIELIAPIAMEKGLRFAIREGGRTVGAGTVSE
                     VIE"
BASE COUNT          203 a          479 c          242 g          334 t
ORIGIN      
        1 gtccgacaaa cttcaccttg tcgcttactt cgtctcgcac tccgacaaac cggagtttgt
       61 ccggcgtttc tactcgatta cttccgacac cgtccctgcg cctaccgttc gcccgccttc
      121 tcttatcgca aatctcaacc ccttctccat cgctatcggc gctatcaact cgatcgtcag
      181 ctgcgagttg tctccgggca tcaccatctc taccccttcg ttcaacgtcg ccaccccagt
      241 cacgtccgtt gtcctgaagt agaactgcgg ccggtacccg ctgaaaaacg gcgtgtgtct
      301 ccctccttct tccttcgtca gcacgtatac ttctgccttg aacttcgtgt gcggcgtgat
      361 cgatccaggc ttcgctatca cctgcccccg ctcgatctcc ttccgctcca ctcctctcag
      421 caacagccct acgttgtctc ctgccattcc cgagtcaagc aacttcttga acatctccac
      481 tcccgtcacc accgacttcc tcgttgcctt gatcccgatg atctctactt cctctcccac
      541 ctttacctgt cctcgctcga tcctccccgt tgccaccgtc cctcgccccg atatcgagaa
      601 aatgtcctcc acaggcatca aaaacggctt gtcgatctct cgcgccggct gcgggatata
      661 gctgtctact gcttccatca gctcttctat ccccttctcc ccatctcctt ccccgttcag
      721 cgcctgcaat gcgcttcctc tcacgatcgg tatctcatcc ccaggaaacc cgtagctcgt
      781 cagcaattcc ctcacttcca actcaaccag atccagcaac tccggatcat ccaccgcatc
      841 caccttgttc atgaacacca ccatcgccgg cacccccacc tgtctggcca gcaatatgtg
      901 ctctcgcgtc tgcggcatcg gcccgtcagt cgccgccacc accagtatcg ccccgtccat
      961 ctgcgccgcc ccagtgatca tgttctttat gtagtccgcg tgtcccggac aatccacgtg
     1021 cgcgtaatgt cggttctttg tctcgtactc cacgtgcgcc gtcgctatcg tgatccctcg
     1081 cgccttctct tccggcgcgt tgtctatcga gtcgaacgat cgaaactgga tcttcgggtt
     1141 gttcttcccc agcaccttcg tgatcgccgc cgtcagcgtc gtcttcccgt ggtcgatgtg
     1201 cccgatcgtc cctacgttca cgtgcggttt tgaacggtca aatttttcct ttgccata
//
LOCUS       DATIFR010000241         1103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2275186, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000241 DATIFR010000000
VERSION     DATIFR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275186"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VLD60_13925"
     CDS             <1..413
                     /locus_tag="VLD60_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46108.1"
                     /translation="RLFSDANRILLRPRYHTLWAFLWLWPMKATIDLRVEGDSIVLSS
                     TKRIPWCSAILTGAWFLIVGLGTVVTLISYVMEGGLARSGGVLIGAGILTLGLIFLFS
                     GLVTVVMANRLENSRLTRVYEELREAIEGGGATT"
     gene            complement(403..747)
                     /locus_tag="VLD60_13930"
     CDS             complement(403..747)
                     /locus_tag="VLD60_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46109.1"
                     /translation="MPEPTAPSSDVIQDLREEFRSHLETFYAQLKLAPPYESVEKAIR
                     ALTTSVHALPKSEQARIAADPALRWDQFRQAFEASGLSKKHRGIIAGLARNRSNLDLP
                     ATYDRFLSLFMS"
     gene            838..>1103
                     /locus_tag="VLD60_13935"
     CDS             838..>1103
                     /locus_tag="VLD60_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46110.1"
                     /translation="MRSTQWAALSLASLMVLGGCSSYHHHGMADGAKSDAYWQKGQQD
                     MEGLIDRTVKDQAKAQQVKSIVGEIVTELKAGREQERAYHRQLYT"
BASE COUNT          229 a          261 c          369 g          244 t
ORIGIN      
        1 ctcgattatt ctcggacgcc aatcgcattc ttctgcgccc gcgttatcac acgctgtggg
       61 catttctgtg gctgtggccg atgaaagcga ccatcgatct gcgggttgaa ggcgattcga
      121 tcgtactgtc gtccacaaaa cgtattccct ggtgctcggc gattctcacc ggagcgtggt
      181 tcctcattgt gggactcggg acggtggtga ctctgatcag ctacgtgatg gagggaggat
      241 tggccagatc cggcggggtg ctgatcggcg ccggtattct gacattaggg ctgatctttc
      301 tgttttccgg attggtgacc gtggtcatgg ccaatcgcct ggagaatagc cggctgacaa
      361 gggtgtacga ggaactgcgc gaggcgatcg aaggcggggg cgctacgaca taaagaggct
      421 gagaaaccga tcgtacgtgg ccggcagatc caggtttgag cgattgcgtg ccagaccggc
      481 gatgatgccg cgatgcttct tgctcaagcc agaggcttcg aaggcctggc ggaattggtc
      541 ccaccgcaag gcaggatcgg cggcaatgcg cgcttgctct gatttcggca gggcatgtac
      601 ggaggttgtg agtgcacgaa tcgccttctc cacgctttca tacggaggcg cgagtttcag
      661 ctgagcgtag aatgtttcga gatgagatcg aaactcttcg cgcaggtcct gaatcacgtc
      721 ggatgatgga gcagttggtt caggcatcgt gatcatgcgg atgatacggt atgatggttg
      781 atcgtgacaa caggtggctg gtttaactag atctgcaaca tggaagggga ggcatctatg
      841 cgatccacgc agtgggcggc gttatcgttg gcgagtctaa tggtgctggg cggctgttcg
      901 tcctatcatc atcatggaat ggccgatggg gcgaagagcg atgcctattg gcagaagggc
      961 caacaggaca tggagggctt gatcgaccgg accgtgaagg atcaagccaa ggcgcaacag
     1021 gtgaagagca tcgtcggcga gatcgtgacg gagttgaagg caggccgcga gcaggaacgt
     1081 gcctatcatc gccagctcta tac
//
LOCUS       DATIFR010000242         1411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_362848, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000242 DATIFR010000000
VERSION     DATIFR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362848"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..256
                     /locus_tag="VLD60_13940"
     CDS             68..256
                     /locus_tag="VLD60_13940"
                     /inference="COORDINATES: protein motif:HMM:NF022890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HSB46111.1"
                     /translation="MPAKSKAQQKAAGIALSAKKGETKKSDLKGPSKSMYESMSEDQL
                     EEMASSKRKGKSEHKSKT"
     gene            complement(318..1153)
                     /locus_tag="VLD60_13945"
     CDS             complement(join(318..784,784..1153))
                     /locus_tag="VLD60_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020875.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSB46112.1"
                     /translation="MWTPEDRALVGDFGSGQALSDDQYRLIEPFIPPPKPGGRPRTTD
                     MRLMLDGLFYVVRTGSQWRHLPPAFPPWQTVYGYFRTFLTTGVWETIRHHLVMTLRES
                     EGREASPTAAIIDTQSVKTTEKRGPCGYDAAKKVKGRKRHVAVDTLGLLLGVIVHAAD
                     IQDADRAWDLLKKVKQLYCWLKVVFADSIYNRVTVLLACFLLGLTLIIVRRMAGTTGF
                     VVIPRRWIVERTLGWLGRWRRLSKDYEELPEVSQAMVTLAMIRLMLHRLVHPNRKGLP
                     AS"
BASE COUNT          321 a          384 c          435 g          271 t
ORIGIN      
        1 ctttgacgca caggcggcgt tttgaaagac tccgctgcgt gcatagctaa agtcactgga
       61 ggcaaacatg ccagcgaagt cgaaggcaca gcagaaggcg gcgggaatcg ctctttccgc
      121 gaagaaggga gagacgaaga agagcgatct gaaggggccg tcgaaaagca tgtatgagtc
      181 gatgagcgag gatcagctcg aggagatggc ctcgagcaaa aggaagggaa agtccgagca
      241 caagagcaag acctgacgtc gggcggcaga gttgattgcg cgtcgcaccc gcacggttta
      301 agtgcccgtt tggaaagtca ggaggccggc aggccctttc ggtttgggtg aacgaggcgg
      361 tggagcatca agcggatcat ggcgagggtg accatggctt gggagacctc aggcagttcc
      421 tcataatcct tggacaaacg tcgccatcga ccgagccagc cgagtgttct ttcaacgatc
      481 catctgcggg ggatcacgac gaagccggtt gtcccggcca tgcggcggac gatgatcagg
      541 gtcaggccga ggaggaagca ggcgagcagg acggtgaccc ggttatagat gctgtcggcg
      601 aacacgacct tgagccagca gtagagctgc ttgaccttct tcagcaaatc ccaggcgcgg
      661 tcggcgtcct ggatatcggc ggcgtgaaca atgacgccca ggagcaatcc cagtgtatcg
      721 accgcgacat ggcgtttgcg ccccttcact ttcttggcgg catcgtagcc gcaaggcccc
      781 ctttttccgt agtcttgacg ctctgggtgt cgatgatggc ggcggttggg ctggcctcgc
      841 ggccctcgct ctcgcgcagc gtcatgacca ggtgatggcg gatggtctcc cacaccccgg
      901 tggtcaggaa ggtgcgaaaa tagccgtaga ccgtctgcca aggcggaaat gccggcggca
      961 ggtggcgcca ctgagaaccg gtgcgcacga catagaacag cccgtccagc atcagccgca
     1021 tatcggttgt ccggggccga ccgccaggct tgggcggcgg aatgaacggc tcgatcaggc
     1081 ggtactggtc atcgctcaag gcttgaccgg acccaaagtc tccaaccaga gcgcgatctt
     1141 cgggcgtcca catcagcgac ctccatcaga agggtttccc ctccatacag ccgtcgctcc
     1201 atctcatcac catcactcag gacagaatca cgaccttttc aaacgggctc ttatatggaa
     1261 ggtacggtca caattccgcg taacacaggt tctgcgatgc caggctcaag cgcaaagttt
     1321 gtggcgaatg cattctgacc gctttaaaag tttaatgttt tgtttatctc gatttaatct
     1381 attgtgcatt gcaaaaaaat ctacatgaag c
//
LOCUS       DATIFR010000243         7982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_363520, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000243 DATIFR010000000
VERSION     DATIFR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363520"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VLD60_13950"
     CDS             complement(<1..932)
                     /locus_tag="VLD60_13950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB46113.1"
                     /translation="MVDYRHILFIKPSSLGDIVHAMPTLAALRQAHPASKLTWLVKRQ
                     WAGIVERIHGVDRVWSVDSGLMGWLSEVPALRAEHFDVAVDLQGLFRSAIMGWLSGAP
                     HRVGFANAREGGPWWYNQPVSVPTTEMHAVDRYLLVAKAMGAEHPGQAEFRFRIPQAD
                     RDEVARLLGRAGVGIGSNWVAMNVSARWPTKRWPAASFAAVADQLNEGGFGPVLFIGG
                     PEDRPVIQTVSGIMKTGAINIAGASSVGLLPALLSQAALLITNDSGPMHLAAAVATPV
                     VALFGPTSATRTGPYGAGHRVLATELPCRPCFNRT"
     gene            complement(996..2726)
                     /gene="waaF"
                     /locus_tag="VLD60_13955"
     CDS             complement(996..2726)
                     /gene="waaF"
                     /locus_tag="VLD60_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSB46114.1"
                     /translation="MHRRPGGRRFVRKQTAKHIVVRGPNWLGDAVMCEPALRGLRRIA
                     PEARISLLVKPAVAELFKVHPAIDQLLVYDDNGRHAGWSGKWILARELRLARFDLAVL
                     FQNAFEAALLAYAAGVPRRYGYATDGRSVFLTEPVAVPDRHAPVHQVEYYWNLLKPMG
                     LTGEPSMPELYLAHDETQAMADRLEQAGIGEKDMVIGLNPGSTYGGAKRWLPERFAEV
                     TNRLCQTLAQSQGKRVSAMILGAKGEELLGGEIAARLSLPSVVLSGSTTIRELMAATK
                     RCALLLTNDTGPMHIASALRVPVVAIFGPTDWRTTSPYGEEQAIVRQPVECAPCLLRE
                     CPIDHRCMTRISSDQVYEAAVKRLTGLEAKGERLGETSPPASRLTRDGVLDGFTVFLD
                     RDGTLNYDPGYLTSAAELSLLPGVAKALARLKAAGAKLVVVTNQSGVGRGLFTLKDLE
                     TIHARLQGLLEQEGAVLDAIYFCPHHPDDGCLCRKPGRAMIDRAVTELQLDLRRSYFV
                     GDHLRDMQLARIVGAKPILVTTGAVDAEALDRLGEEQGLPDKVAGSMVEAVDWIFADV
                     KTRLPLTVNR"
     gene            complement(2689..3774)
                     /gene="lpxK"
                     /locus_tag="VLD60_13960"
     CDS             complement(2689..3774)
                     /gene="lpxK"
                     /locus_tag="VLD60_13960"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSB46115.1"
                     /translation="MLFTPGSRVRWWFLWVAIPYGIVAGLRARCYQWGLFAQKKLPVS
                     VISIGNLTLGGTGKTPVVIRLAEWLLAEGRRVAILSRGYKRTSHDKMLLVSNGMQLLA
                     GPQDAGDEPFLIATRCPQAVVAVGADRYELGRWVLSQFPLDCILLDDGFQHLSLHRDV
                     NLLLVDATDVNGLDAVVPAGRLREPITSAARATLIVVTRADVAAQAAQVVQRLREAIG
                     LLPDPVQVVFRAQGFLSLTTGEVRPLSWCQGKRAVLCSGVGHGASFRATAEGMGLCLL
                     DEVRYPDHHLYAPADIELVRVRAKELNADLIVTTEKDAGKMVPLLTENDDNWWAIRLD
                     TEVTVGADRLRQVIRNASSPRRTEVCA"
     gene            complement(3777..5120)
                     /locus_tag="VLD60_13965"
     CDS             complement(3777..5120)
                     /locus_tag="VLD60_13965"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HSB46116.1"
                     /translation="MWHLFYNALLLIAAPIILVILLAKQRCRRGFSERCGFRIPAIRA
                     DHRPLIWVHAVSLGEAVAVSPLVKELHRRHPDHRFVVSTVTETGREAVEERLAGVAEH
                     LYAPLDFPWVVSRLVNQLRPVLYLFVETELWPNLLRELRRLGVPTVMVNGRLSTRSFS
                     RQQWGPVRSFYRTMLRGLSLCLMQSDRDAERIIALGADARSVRRTGNIKFDQPMPASV
                     DSESIRSSLRLQEGERLFVAGSTHPGEEEALVDCYRSLVAQVPSAIMLLAPRHIERVD
                     QVEAMIRARGVPVQRRSLMGQAQSLAPVGPRVIILDSRGELASLYREAVVAYVGGTLI
                     PVGGHNLLEPAVWAKPVLFGPYTDHCAEIASLLLDAGGGRLVRDAGDLIREVIQLFEH
                     QEECAKMGRSARLVVEQNQGALRSTVEAIETLLTPHQTGTETDHPGNRVPLTAGR"
     gene            complement(5277..5861)
                     /locus_tag="VLD60_13970"
     CDS             complement(5277..5861)
                     /locus_tag="VLD60_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSB46117.1"
                     /translation="MGKPLLKPVETWLKLSLLPPIGAAVIRGLGRSMCIEVRGQEHVD
                     ALYRSGKNIILSFWHAQQLMIPLGYRGPGSHVLISQHQDGEIIARIIAAFGHQAVRGS
                     STRGGAAALRELIRLGRSGKDVVVTPDGPKGPAQVAKMGVIQLARASGLPIVPLAFGC
                     SKKNTSRVGIDSWSPFLGLAASFCGGLRSTYLGN"
     gene            complement(5854..7599)
                     /gene="msbA"
                     /locus_tag="VLD60_13975"
     CDS             complement(5854..7599)
                     /gene="msbA"
                     /locus_tag="VLD60_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HSB46118.1"
                     /translation="MSSLDRFIRLLRYLRPYRVRLIAAFLCSGLVAAFSGAYAWLVKP
                     VLDEIFINKNEALLLALPLALFAVSVLKSAFNYGQNYLMNYVGNQVITDIRQELFGKL
                     IRLPVKYHDVNASGRLVSRVVNDVSLMANAVAGVLKDLFQQGLTFLAMMGVIFYQNWK
                     LAGLSIIVIPLAVVTMIRMGKRLRMLATSGQERMGDMASTLQETLSGIRMVKAYGREE
                     TEAERFRRSNRAFLDTTMKAIQVSSLGSSHMEVIGVVGVAAIIWYGGYLVIHGDMTPG
                     AFFSFLTAMFMAYTPLRRLSGSNNTIQQALAAAERVFSVLDLETEQAADHGRLTLPPV
                     ARSVVYQDVTFLYESQTVPALSDIDLTIRAGEMVAIVGSSGSGKTTLVNLLPRFYEPT
                     TGQILIDGVDIQSCTLASLRAQIGIVAQDVVLFDDTVSNNIAFGRREATEESIRQAAR
                     LAYAHDFIERLPQGYQTVVGEKGVKLSGGERQRLAIARAILRDPPLLILDEATSALDT
                     ESERVVQLALSNLMQNRTTVVIAHRLSTIQRADRIVVMDRGTIVETGTHDELLLRGGQ
                     YKRLHAMQFQDMPNG"
     gene            complement(7587..>7982)
                     /locus_tag="VLD60_13980"
     CDS             complement(7587..>7982)
                     /locus_tag="VLD60_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSB46119.1"
                     /translation="VRVVRDQASEVMAAADVLLIASGTATLQAAVVGTPMVLLYKTTP
                     LTYRLARWLIRVKWIGLVNLVAGRSVVPEVIQDEATADRLSQEVLHLLTDSNAYNAMK
                     QELQQVRQSLGSPGASRRAAQVVLAECQV"
BASE COUNT         1761 a         2526 c         2180 g         1515 t
ORIGIN      
        1 gtccggttga aacaaggccg acaaggaagc tcggtagcca gaacgcgatg gccggcccca
       61 tagggaccgg tccgcgtcgc acttgtcggc ccgaacaacg ccacgaccgg cgtggctaca
      121 gcggccgcaa ggtgcatcgg cccagagtcg ttggtaatca ggagagccgc ttggctcagc
      181 agcgcaggca acagcccgac cgacgaggca ccggcgatat tgatcgctcc ggtcttcatt
      241 atcccgctga cggtttgaat gactggccgg tcttcaggtc cgccgatgaa cagcaccggg
      301 ccaaagcccc cttcattcaa ctgatccgcg acggctgcaa acgatgcggc gggccaccgt
      361 tttgtcggcc atctggcaga cacgttcatc gctacccagt tcgatcctat cccgaccccg
      421 gcacggccca aaagacgagc aacctcatcg cgatcggctt gtggaattcg aaaacggaat
      481 tctgcctggc caggatgctc agcgcccatc gctttcgcca ccaacaagta gcggtcaacg
      541 gcatgcatct ccgtcgttgg aaccgagaca ggctggttat accaccaggg gccgccttca
      601 cgcgcgttgg caaatccaac tcgatgagga gcaccactca accaccccat aatggcactc
      661 cgaaacagcc cttgcaggtc gacggcgacg tcgaaatgct cggctcgcag ggccggcacc
      721 tctgatagcc accccatcaa tccggaatcg accgaccaga cgcgatcgac gccgtggatg
      781 cgttcgacaa taccagccca ttgacgcttc accagccacg tgagcttgga ggcaggatga
      841 gcctgacgca aggcggccag ggttggcatt gcatgcacaa tatcaccgag ggaactcggc
      901 ttgatgaaga ggatatgtct gtagtccacc atgagcgtta agcgttattc gtgaatcgta
      961 agaccaagca gcatgcgcat tcgaccgatc agcggttaac gattcacggt taacggaaga
     1021 cgggtcttca catcagcaaa aatccaatca actgcttcga ccatggagcc tgcgaccttg
     1081 tccggtaatc cctgctcctc ccccaagcga tcgagcgcct ccgcatccac ggctccagtt
     1141 gtcacaagga tcggcttcgc ccctacgatt ctggctagtt gcatgtcccg tagatggtca
     1201 ccgacgaaat acgatcgacg aaggtccagc tgcaactccg tcacggcccg atcaatcatc
     1261 gcgcgtccgg gcttgcgaca gaggcatccg tcgtccggat ggtggggaca aaaataaatc
     1321 gcgtcgagca ctgcgccttc ctgttccagc aacccctgca gtctcgcatg aatcgtctcc
     1381 aagtccttca aagtgaacag gccgcggccg actcccgact gattcgtgac gaccaccagc
     1441 tttgcgccgg ccgccttcag ccgagccaaa gctttggcga ctcccggtag aagactcaat
     1501 tctgctgccg aggtgagata tccgggatcg tagttcaacg tgccgtctcg atcgaggaaa
     1561 acggtgaagc catcgagcac cccgtcacgc gtcaggcggg aggcgggagg tgaggtttcc
     1621 cctaaccgct cacccttcgc ctctaacccg gtcaaccgtt tcaccgccgc ttcgtacacc
     1681 tgatccgagg agatccgcgt catacaacgg tgatcgatcg gacattcgcg caagaggcag
     1741 ggtgcgcact cgaccggctg tcgaacgatg gcttgctcct ccccatacgg tgatgtcgtc
     1801 cgccaatcgg ttggaccgaa gatcgccacg accggcaccc gcaaggccga cgcgatatgc
     1861 atgggccctg tgtcgttggt cagcagcaac gcacagcgct tggtggcggc catgagctcg
     1921 cgaattgtgg tcgatccgga caagaccacc gagggcaacg acaaccgcgc ggcaatttca
     1981 cctccaagca gttcttctcc cttggctccg agaatcatcg ccgaaactcg tttcccttga
     2041 gattgagcga gtgtctggca caatcggttc gtcacctcgg caaatcgctc cggtaaccat
     2101 cgcttggcac cgccgtatgt cgaaccggga ttgaggccga tgaccatatc cttctcgcca
     2161 atccctgcct gctcaagacg atcggccatg gcctgtgtct catcgtgggc aagatagagc
     2221 tcgggcatgg aaggctctcc cgtcagaccc atcggcttga gcagattcca atagtattcg
     2281 acctgatgca cgggagcatg gcgatccggg acggccactg gttctgtgag aaagacgctc
     2341 cgcccgtccg tcgcataccc atagcgccgc gggactcccg ccgcataggc caacaacgcc
     2401 gcctcaaacg cgttctgaaa caacaccgcg aggtcgaatc gagcgagtct gagctccctt
     2461 gcaagtatcc attttcccga ccatcccgca tggcgcccat tatcgtcata aacaagcagc
     2521 tggtcgatcg ctggatggac cttgaacaat tcggcaaccg ccggcttcac caagagtgaa
     2581 atcctggcct ctggcgcaat cctcctcaga ccccgcagcg caggttcgca catgaccgca
     2641 tcaccaagcc agttaggccc tcgaaccacg atatgttttg cggtttgctt acgcacaaac
     2701 ctccgtccgc ctgggcgacg atgcattccg aatcacttgc cgaagccggt ctgctcccac
     2761 tgttacctct gtgtccaaac gtatggccca ccagttgtca tcattctcgg tcagcaacgg
     2821 taccattttc ccagcatctt tttcagtggt gacgatcagg tcggcattca gctctttggc
     2881 tctgacacgc accagttcga tgtctgccgg cgcgtagaga tggtgatcgg gatatcgaac
     2941 ctcatccagc aggcagaggc ccatcccttc agccgtggca cggaacgaag ccccatggcc
     3001 gaccccgctg cacaacacgg ctctctttcc ctgacaccac gaaagaggac ggacttcacc
     3061 ggtggtaagc gacaggaacc cttgagcccg aaagacaact tgcacaggat caggcaggag
     3121 accgatagcc tcgcgcaacc gctggaccac ctgtgcagct tgagccgcca catcagcccg
     3181 cgtcacgacg atcaacgtcg cgcgcgcggc ggaagtgatc ggctcgcgca aacggccggc
     3241 cggcaccact gcgtccagtc cgttcacgtc cgtcgcatcc accagcaaca gattcacatc
     3301 gcggtgaaga ctcaaatgct gaaagccgtc gtcgagcagg atgcaatcca gagggaattg
     3361 actcaagacc catcggccaa gctcgtaccg atcggcgccg actgcgacca ccgcctgcgg
     3421 acaccgtgtc gcgatcaaga atggttcatc gccagcgtcc tgtggcccag ccagaagctg
     3481 catgccattc gagaccagga gcatcttgtc atggctggtc cgtttgtacc ctcgactgag
     3541 aatcgcgaca cgcctcccct ctgccagcag ccactcggcc aaccggatca caacgggagt
     3601 ctttcccgtc ccgccgagcg tcaggtttcc gatactgatt acggaaacgg gcagtttctt
     3661 ctgtgcaaat aatccccact gataacagcg ggctcggagt ccagcgacaa ttccataggg
     3721 aatcgcaacc cagaggaacc accatcggac tcgcgatcca ggagtgaata acatccctat
     3781 cgtcctgccg tgagggggac ccggtttccc ggatgatcgg tttcggtacc ggtctgatgt
     3841 ggtgtcagca gggtttcgat cgcctccact gttgaccgca gggctccttg attctgttca
     3901 acgacgaggc gggccgaccg gcccatcttc gcgcactcct cttgatgctc gaatagttga
     3961 atgacttcac gtatcagatc tcccgcatct cttaccagtc ggccaccccc tgcatcgaga
     4021 aggagagacg cgatctcagc acaatggtcc gtgtatgggc cgaagagaac cggcttggcc
     4081 cagacagcgg gctcgagcaa gttatgtccg ccgaccggaa tgagcgtgcc gccgacatag
     4141 gccaccacgg cctcccgata cagtgaggcc agctcgccgc gcgaatctag gatgatcaca
     4201 cgcggtccaa ctggagctag agattgggcc tgacccatca ggcttcggcg ttggactggt
     4261 actccgcgtg cacgaatcat cgcttcgact tgatcgaccc gttcaatatg ccgtggagcc
     4321 agcagcatga tggctgaagg cacttgcgcc accagactcc gatagcaatc gacgagggct
     4381 tcctcttcac ccgggtgcgt acttcctgcg acgaacagcc gctctccctc ctgcaatcgt
     4441 aggcttgacc tgatggattc cgaatctaca gaagcaggca tcggctgatc gaacttgata
     4501 tttccagtgc gccgcacgga ccgcgcatcc gcgccaagcg caatgatccg ttccgcatcg
     4561 cgatccgatt gcatcagaca caaactcagc ccccgcaaca tcgtccgata aaatgaccgg
     4621 acaggtcccc attgttggcg agaaaaagac ctggtggaca atcgtccgtt caccatgacg
     4681 gtcggaaccc cgaggcggcg aagctctcgt aacaaattcg gccacaactc ggtctcgacg
     4741 aagagataga gcaccggccg caattggttg accagtcgcg acaccaccca cgggaagtct
     4801 aggggggcat aaagatgctc agccacaccg gccaatcgct cctcgaccgc ttctcgtccc
     4861 gtttccgtca cagtcgagac gacaaagcga tggtccgggt gacgccggtg cagttccttt
     4921 accaacggcg agaccgccac cgcctcaccg agcgacaccg catgaaccca gatcagcggc
     4981 cgatgatcgg cacggatggc gggaatacga aacccgcatc gttcagagaa accgcggcgg
     5041 caccgttgtt tggcaagcaa gataaccaga atgatcggag ctgcgatcag gagcaacgcg
     5101 ttatagaaga gatgccacat aacgttgtgg gttaggcgtg actcgtgagg ccacgtgcct
     5161 cacctcctga gtcctctggc gtcacgtctg gctattcacc gcccgatcag cctccgcagt
     5221 catgcggttc aagaccgtct caagttctaa ccgctttact tcgagcgctg attcgtctag
     5281 ttcccgaggt acgtagatcg gagcccccca cagaaagagg ccgcgagacc aaggaaaggg
     5341 gaccatgaat cgatcccaac tcgcgaagtg tttttttttg agcagccgaa ggccaacggt
     5401 acgatcggga gaccggaagc gcgcgctaac tggatcactc ccatcttcgc cacctgcgcc
     5461 ggccccttcg gcccgtccgg cgtcacgacc acgtccttgc cggatcgacc gagccggatg
     5521 agctctcgca gtgcggccgc tccccctctc gtactcgatc cccgcaccgc ctggtggcca
     5581 aacgcagcaa tgatccgggc gatgatttct ccatcctggt gctggctgat gagcacatga
     5641 gatccggggc ctcggtagcc gagcgggatc atgagttgct gggcatgcca gaatgagagg
     5701 ataatgtttt taccgctccg atagagcgca tcaacatgtt cctggccgcg cacctcgatg
     5761 cacatcgatc gacccagtcc tcgaatcact gctgcgccga tcggaggaag gagcgagagt
     5821 ttgagccagg tttccaccgg cttcagaagc ggcttaccca ttgggcatat cctgaaattg
     5881 catggcgtga agccgcttat actgccctcc gcgaagtagc agctcgtcat gggttccggt
     5941 ctcgacgatc gtgccgcgat ccatgaccac aatccgatcg gcccgctgta tcgtcgacag
     6001 gcgatgggcg atcacgactg tggttcggtt ctgcatcaaa ttactcaacg ccaactggac
     6061 aacgcgctcg gattcggtgt ccagtgcaga cgtcgcctca tcgagaatga gcaagggcgg
     6121 atcgcggaga atggcgcggg cgatcgccaa ccgctgacgc tcacccccgg aaagcttcac
     6181 acccttctcg cctaccactg tctggtagcc ctgcggaagc cgctcgataa aatcgtgcgc
     6241 ataggccagt ctggccgctt gcctaatcga ttcctcggtg gcctctcgcc gtccgaatgc
     6301 gatgttattg ctgaccgtgt cgtcgaacaa cacgacatcc tgggcgacga taccgatctg
     6361 ggcgcgcagg gacgccaacg tacaggactg gatatctacc ccgtcgatca gaatctgccc
     6421 ggtagtgggc tcgtagaacc gagggagcag attcaccagt gtcgtcttcc cgctgccgct
     6481 gctgccgaca atggcaacca tctcaccggc ccggatcgtc agatcaatgt cggacagtgc
     6541 cgggactgtc tggctttcat agagaaaggt cacatcttga tagaccaccg accgagccac
     6601 cggcgggagg gtgagccggc catgatcagc ggcctgttcg gtctcaaggt cgaggacgct
     6661 gaatacgcgc tcggctgctg caagcgcctg ttggatcgta ttgttcgaac ccgacaaccg
     6721 gcgtaatggc gtataggcca tgaacatggc cgtcaagaat gagaagaacg cccccggcgt
     6781 catgtctccg tgaatcacaa ggtacccgcc gtaccagatg atggccgcca cgccgaccac
     6841 tccgatcact tccatgtgcg atgatccgag cgaagacacc tgaatcgcct tcatcgtggt
     6901 atcgagaaac gcccggttgc ttcgtcgaaa tcgctccgct tcggtctctt ccctcccgta
     6961 ggccttcacc atgcggatgc cggagagggt ttcctgcagt gtcgaagcca tatcgcccat
     7021 ccgctcttgc ccgctggtgg ccaacatgcg aaggcgcttg cccatccgta tcatggtcac
     7081 cactgctaat ggaatgacga tgatcgacaa gccagccaat ttccaattct ggtagaaaat
     7141 aacccccatc atggcgagaa aagtgaggcc ttgctggaag aggtctttca acacgccggc
     7201 gaccgcgttc gccatcaatg acacgtcatt cacgacgcgg gataccagac gacccgaggc
     7261 attcacatcg tgatacttga ccggcaatcg aatgagtttg ccgaacaact cttgacggat
     7321 gtccgtaatc acctggttac cgacataatt catcagataa ttttggccgt aattgaaggc
     7381 gcttttgagc acagacacgg caaagagggc gaggggaagg gccagcagca gggcttcgtt
     7441 cttattgatg aagatttcgt cgagcaccgg cttgaccagc caggcatagg ctccggagaa
     7501 ggccgcaacc agccccgagc agagaaaggc cgcgatcagc cggacccgat agggccgcag
     7561 gtatcgaagg agccgtatga aacgatctag acttgacatt ccgctaacac aacctgggcg
     7621 gcacgacgag aagcccccgg gcttccaagc gattgtctaa cttgttgcag ttcttgtttc
     7681 atagcattat acgcgttgga atcggtcaga aggtgaagaa cctcctggct caagcgatcc
     7741 gccgtcgcct catcttgaat gacctcaggc acaacggaac gcccggcgac aagattcacg
     7801 agcccgatcc atttcactct gatcaaccag cgggccagcc gataggtcag cggggtagtt
     7861 ttgtacaaca ggaccatggg agttcccacc accgcggcct gcaaggtggc cgtcccggac
     7921 gcgatcagca agacatccgc ggcagccatc acttcgctgg cctgatcccg gacgacgcga
     7981 ac
//
LOCUS       DATIFR010000244         4474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1131105, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000244 DATIFR010000000
VERSION     DATIFR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131105"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VLD60_13985"
     CDS             complement(<1..754)
                     /locus_tag="VLD60_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46120.1"
                     /translation="MSTVVSQDQEALNAEIAEVREKITRLKKKLRAVEAELEGMSHDR
                     ERYQLLNDICQGLDKLEELGAAKLFWGERAGKPDATGHVSRVREAASEFVQKLAGVEE
                     RRRILQDQIEKQLLRFDLLSDELIELQEREEESKYEFVINRELILPPYSPTILPWSEE
                     REDQRRFRKSLLVSLLVALCFGILMILWKLPLRDTADVTEIPERLVKLVQRQMPKPPE
                     PKPLEKKKPEDETKPKQKTETQQARAKAEQSGL"
     gene            complement(767..1261)
                     /locus_tag="VLD60_13990"
     CDS             complement(767..1261)
                     /locus_tag="VLD60_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB46121.1"
                     /translation="MNNTRRLERMARARRHTAGLNLTSLMDVFTILVFFLLVNQSSVE
                     VIEPPKQIKLPDSVVEAKPRQTVTILVSADAILVQGEPVVSVAEVLGMKDENIKAVSE
                     KLAQLRENIIGINTATVARSNEVTILSDKAIPFKVLKRLMSTCTNTGYTKISLAVIQK
                     ASQS"
     gene            complement(1258..1746)
                     /locus_tag="VLD60_13995"
     CDS             complement(1258..1746)
                     /locus_tag="VLD60_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB46122.1"
                     /translation="MSRLHHYKRHKETPELDMTTFLNLMVALIPFLLLTAVFSRITIM
                     DLNLPTAAGGPSPDKPQVTIEVIVRKNALQIGDGRHVVVSIPKESDKYDLKKLSEYLM
                     RLKSTYSQKDDATVLIEPDIEYEDMIHVMDAVRVIEVKQPDREETQKVALFPAISIGD
                     AP"
     gene            complement(1749..2411)
                     /locus_tag="VLD60_14000"
     CDS             complement(1749..2411)
                     /locus_tag="VLD60_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSB46123.1"
                     /translation="MNVIETIVRFIQDGGAFMYPILVVGAVGFAISIERFIKLLLVRK
                     ANQAMWEQLHPVLVEGKFDKARKMTAEDKSTVSHLLSLGLAREGAVRRRDDIEIAMEE
                     GMMEIIPQLEKRTSYIALFANIATLLGLLGTIIGLIQAFTAVAHANPAEKADLLSASI
                     SVAMNTTAFGLIVAVPLLLAHAYLNSRAGEIIDSLEMASVKTLNIISSTTQRKYQVDE
                     AA"
     gene            complement(2452..2748)
                     /locus_tag="VLD60_14005"
     CDS             complement(2452..2748)
                     /locus_tag="VLD60_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46124.1"
                     /translation="MSRNVIIAIVLCALHLSAIAQDRAGTGAKGAGTEAKELSGMSVL
                     GNTDMPKALYIVPWKTSEMGLTTDLRSGLLNDAMVPVDKEVFMHELEYYEISSR"
     gene            complement(2757..3398)
                     /locus_tag="VLD60_14010"
     CDS             complement(2757..3398)
                     /locus_tag="VLD60_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB46125.1"
                     /translation="MNFTLLYNTSLGNVTALLALVLTLVGCAGGPPTVPVNQAIDDTQ
                     KNAVLVDVAADADVAADFEKAMKLLKAGEYEKGTDLMQNVAQRSPGQTAPHINLAMAY
                     EKMGKLPAAEESIKKALELSPEHPVANTVYGLLYRKTGRFAEARQVYERALKQYPDFL
                     AARKNLGILCDIYLRDLECALTQYHVYSTAMPDDKTVRVWIADLEQRRAKQAP"
     gene            complement(3792..>4474)
                     /locus_tag="VLD60_14015"
     CDS             complement(3792..>4474)
                     /locus_tag="VLD60_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46126.1"
                     /translation="YLSELRQIVDSDARAGGERTDRTRYLAAVSALALTEPLYERFVE
                     IKLAKPFQKNLARKKAAMKEATDAFGKLAGYQAGEATAAAAFYIAEIYYQFGKALTES
                     DRPDNLSAAEREQYELALEEQAYPFEEKAIKLHEKNLELLSVGVYNAWIDKSIERLAQ
                     LMPARYARAEENPGFIESIGPYRYVLVTKPSSPEASARKDVQPAQSGQLPGTERPEQS
                     ESSAVSAH"
BASE COUNT          799 a         1365 c         1228 g         1082 t
ORIGIN      
        1 ggagccccga ctgctccgcc ttggcgcggg cctgctgcgt ctcggtcttc tgtttgggct
       61 tcgtttcgtc ttcgggcttc ttcttctcga gcggcttcgg ctcgggcggc ttcggcatct
      121 ggcgctggac cagcttgacg agcctctcgg gtatctccgt cacgtcggcc gtgtctcgga
      181 gcggcagctt ccacaggatc atcaggattc cgaagcacag cgccacgagc aacgagacga
      241 gcagcgactt gcgaaaccgt cgctggtctt cgcgttcctc ggaccagggc aagattgtcg
      301 gactatacgg cggcaggatg agctcgcgat tgataacgaa ttcatacttg ctttcctctt
      361 cccgctcctg cagctcaatc agctcatcgc tcagcagatc gaacctgaga agttgttttt
      421 cgatctgatc ctgcagtatc cggcggcgct cctcgacgcc cgcgagcttc tgtacgaatt
      481 ctgatgctgc ctcgcggacc cggctgacat gccccgtcgc atccggcttt cctgcgcgct
      541 cgccccagaa gagctttgcc gcgcccagct cttccagctt gtcgagcccc tggcaaatat
      601 cgttcaggag ctggtagcgc tcccggtcat gcgacatgcc ttccagctcg gcttcgaccg
      661 cgcgcaactt tttcttgagc cgcgtaatct tctcgcggac ttccgcgatc tccgcgttca
      721 gcgcttcctg atcttgagat acgacggtac tcatgtcatc aggccgtcag ctttgggatg
      781 ccttctgaat tacggcgagc gaaatcttcg tgtatcccgt gttcgtgcaa gtcgacatca
      841 gtctcttgag caccttgaat gggatggcct tgtccgaaag aatcgtgacc tcgttgctcc
      901 ttgccacggt cgctgtgttg atgccgatga tgttttcccg caattgagcg agcttctcgc
      961 tcaccgcctt gatgttctca tccttcatgc ccagcacttc cgcgacggac accaccggct
     1021 cgccctgcac gagaatggcg tcagcgctca ccaatatcgt gaccgtctga cgcggtttcg
     1081 cttccactac ggagtcaggc aactttatct gtttcggcgg ctcgatcacc tcgacgctcg
     1141 attgattcac cagcaaaaag aacaccagga tagtaaagac atccatgagc gacgtgaggt
     1201 tgaggccagc cgtgtggcgc ctcgcccgag ccatgcgctc aaggcgtcga gtgttattca
     1261 tggcgcatca ccgatggata ttgccgggaa cagcgcgacc ttctgtgtct cctcccggtc
     1321 cggctgcttc acctcgataa cgcgtaccgc gtccatcacg tgaatcatgt cttcgtactc
     1381 gatgtccggc tctatcagca ccgtcgcatc atccttttga ctgtatgtgc ttttcagccg
     1441 catgagatac tcagaaagtt ttttgaggtc gtacttgtcg ctttcctttg gaatcgacac
     1501 gacgacgtgc cggccgtccc cgatctgcaa cgcgttcttg cggacgatga cctcgatagt
     1561 cacctgtggc ttgtcgggtg atggcccgcc tgccgcagtt ggcagattga ggtccatgat
     1621 cgtgattcgt gagaacacgg ctgtgagaag caggaacggg atcagcgcca ccatcaggtt
     1681 gaggaaggtg gtcatgtcca gttcgggcgt ttccttgtgc cgcttgtaat gatgtaaccg
     1741 cgacatcgtc aggcggcctc gtcgacctgg tatttgcgct gcgtcgtcga cgagatgata
     1801 ttgagcgttt tgaccgatgc catctcgaga ctgtcgatga tctccccggc ccgggagttc
     1861 aaataggcgt gggcgagcag gagcgggacc gcgacgatca gcccgaacgc ggtggtgttc
     1921 atcgcgaccg agatgctggc ggacagcagg tccgcctttt ctgccgggtt ggcatgggcc
     1981 accgcggtga acgcctggat caggccaatg atggttccga ggaggccgag cagcgtcgcg
     2041 atgttcgcga acagggcaat gtacgaggta cgcttctcca gttgagggat gatctccatc
     2101 attccctcct ccatggcgat ttcgatgtca tcccggcgcc ggacggcgcc ctcccgcgcc
     2161 agacccaggc tcaacaggtg gctcaccgtg gatttgtcct cggcggtcat cttcctcgcc
     2221 ttgtcgaatt tgccctcgac gagaaccggg tgcaactgct cccacatcgc ctgattcgcc
     2281 tttcgcacga gcaggagctt gatgaatcgc tcaatcgata tcgcaaaccc gactgcgccg
     2341 acgaccagga tcggatacat gaacgccccg ccgtcctgga tgaaccttac aatcgtttcg
     2401 atcacattca tttttcagcc ctcatagcaa tttccacgtt gacgcttacc cctaacgact
     2461 gctgatctcg taatactcga gctcatgcat gaacacctcc ttgtcgactg gcaccatggc
     2521 gtcgttcagg aggcccgacc tcagatctgt cgtcagaccc atttccgagg tcttccaagg
     2581 aacgatatag agcgctttgg gcatgtcggt gttgcccaac accgacatgc ccgacaattc
     2641 tttcgcctcg gttccggcgc ctttggcccc ggtcccggcg cggtcctgcg caatagccga
     2701 caagtgaagc gcgcaaagaa cgatggcaat aattacgttt cttgacatga cgtttcctag
     2761 ggtgcctgtt tcgcgcgtcg ctgctccaaa tccgcgatcc agacgcgtac ggtcttgtcg
     2821 tcaggcatgg cggtgctgta aacgtgatat tgggtcaacg cgcattccaa atctctcagg
     2881 tatatgtcgc acagtattcc caggtttttc cttgcagcga gaaaatcggg atattgcttc
     2941 aaggcccgct catagacctg ccgtgcttcg gcgaaccttc cagtcttgcg ataaagcaac
     3001 ccgtacacgg tgttggccac cgggtgctcc gggctgagtt ccagcgcctt cttgatactc
     3061 tcttccgccg ccgggagttt gcccattttt tcgtacgcca tggcaaggtt gatgtgcgga
     3121 gcagtctggc ccggagaacg ctgcgccaca ttctgcataa gatccgttcc tttctcgtat
     3181 tcgccggcct tgaggagttt catcgctttt tcgaagtcgg cggcgacatc ggcatccgct
     3241 gctacatcta caagaacggc gtttttttgc gtgtcgtcta ttgcctgatt aaccggaacc
     3301 gtcggcggac ctcccgcgca gccaacgagg gttagaacga gcgccagcag ggcggtaacg
     3361 ttgccgagag aagtgttgta cagcagtgtg aaattcattc tcgtgtgacg tccctgcccg
     3421 ttgcgcattg atagcccgcc ctgtgccgct gacgtcaacg aatgcgcagc gtctatctcc
     3481 ggaatgctgc tccgatggac ggcttcctgc acatgcgcca tcgggcggct ttttgagcgg
     3541 ctatgtagag gagtagtctt ggccctcatg actgtcaaga ctgtcaatac tttcaaagta
     3601 gtgagtcgtc tttcgacaac cgcttggcgg cgcaatatgc catttcatcg gcgttagctc
     3661 cttcagttgc cggcttcacg aaggtcggtt cattcggtcc cgcaattcgg caaacgttcc
     3721 gcatcggtgc gccgtccaat cgtggaattg cgcaatggga gagattcatc actgcctcac
     3781 ggacgatgcg gctaatgcgc agacacggcg ctcgattcac tctgctccgg ccgttcggtc
     3841 cctggcagct gcccggattg cgctggctga acatccttgc gagccgatgc ctccggcgac
     3901 gacggtttcg tgaccagcac gtaccggtat ggcccgatgc tttcgatgaa gccgggattc
     3961 tcctcggccc tcgcataacg ggctggcatc agctgcgcca gcctctcgat acttttgtcg
     4021 atccatgcgt tgtagacgcc cacactcagc agttccagat tcttctcatg caacttgatc
     4081 gctttctctt cgaacgggta ggcctgctcc tccagggcaa gctcgtattg ctcacgctcc
     4141 gcggcgctca ggttgtcggg tctgtcggac tctgtcaggg ccttgccgaa ttggtaataa
     4201 atctcggcga tgtagaacgc tgcggcggcg gtcgcctccc ctgcctgata gccagcgagt
     4261 ttcccgaagg cgtccgttgc ctccttcatg gctgcttttt tccgggcgag gtttttctgg
     4321 aatggcttgg cgagcttgat ctccacgaat ctttcataca acggctccgt cagcgccagg
     4381 gccgacacag cagccagata tcgcgtgcga tcggtacgct cgccccccgc ccgcgcatcg
     4441 ctgtctacga tctgccgaag ctcgcttaag tagc
//
LOCUS       DATIFR010000245         2770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_366367, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000245 DATIFR010000000
VERSION     DATIFR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366367"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VLD60_14020"
     CDS             complement(<1..295)
                     /locus_tag="VLD60_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSB46127.1"
                     /translation="MALTRTIVALRESEERFRTFLDHSPKLAFVKGTDGRYLYVNRRF
                     EEAFHIPTGGALGKTDIELFPRAQAEQFQTHDRQVLESGVAKEFEEVAWQADGF"
     gene            1408..1608
                     /locus_tag="VLD60_14025"
     CDS             1408..1608
                     /locus_tag="VLD60_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HSB46128.1"
                     /translation="MSVAKIIEISSESPKSFEEAIVQGIARASKTVHNIKSAWVKEQH
                     VVINNGKVDMYRVDLKVTFVLD"
     gene            1762..2367
                     /locus_tag="VLD60_14030"
     CDS             1762..2367
                     /locus_tag="VLD60_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB46129.1"
                     /translation="MVIALILVCSAVVNAASQSSESPTEVVRATITEVFRILEDPKLK
                     GPAMVMPRRRMLEEVIASRFDYAEMSKRALAAHWAPLTAAQRGEFVELFKSFLSDRYA
                     EKIEGYNGEQVHYLSERIEGNYAEVRTEIRSSKVEIPMDYRLFLKETQWHAYDIVVDG
                     ISLVKNYRSQFEKIIRTDSYQELLRRLRDRAVTEERKGQRS"
BASE COUNT          623 a          751 c          798 g          598 t
ORIGIN      
        1 ggaagccgtc ggcctgccac gcgacctctt caaactcttt ggccactccc gattcgagga
       61 cctgtcgatc atgagtctga aactgctcgg cctgagcacg cggaaacaac tcgatatccg
      121 tcttgcccag cgcaccccca gtcggaatgt gaaaggcctc ctcgaatcgg cgattgacat
      181 agaggtagcg accatccgtc cccttcacaa aggcgagttt cggcgaatga tcaagaaacg
      241 tccggaaccg ttcctcgctc tcgcgcaagg ccacgatcgt ccgcgtcagc gccatagtcc
      301 gctcctgaat caactgctcc aattgtacga gtcgtggcct gcgggccttt gcagaacggg
      361 gcggcgctcc tgtccgggaa cgatgggggg aagacggcgt cttggctcca cgtttcttct
      421 tggttgattt tgacgacatg gaagaggagg caattctagg ctactggtga ggccctcgca
      481 agggtgccgc ttggtcgtta ttgccgaggg cctgattcca gtgaattttg gcgtgagact
      541 agggaaatcc cgagggcatg agaagctcga gaaatagcat ggttttcggt gggcagaggc
      601 tgttcagtga cctgacggac atggcttctt ccttacatta cgatcgagtc gcgtgcctac
      661 aaggtcggtc gcaacattcc taggcgcctc ccgcaagacc tcaaacagag aagataaccg
      721 ggccaggatg ctgtcgccta ttgcaacagt gccatttccc aagttgaggc accttggtca
      781 atcagactca tgggccagct ccctcaagcc tcattcagca acgtcaaatc cctgtatgat
      841 caacgtcctc tcaattcagc cttgccggag tcgttcggca tggtgcttgc tgttcagctc
      901 actgtcatag gcgtggcgtt cacgcaagga ggggccggtg tcttcggaag aaaccgcaac
      961 gcatccaatc acggtctctt caagccagca tccaagcatg acggcgcggc ttgttcctgt
     1021 ttgctgtctc tgcaggctca ttcgcgacga gccgggatct ctccccagcc acatgtcatg
     1081 ggtcactctg gggtcgtatc gaaaagctca caacatagat tcgaccgagc ttctcttcac
     1141 gcatacctat tgtccggact gcctcgtgca agtccaggag agagtacgag aacatttcag
     1201 gtcgaagaga gccgaagccc ggacttcttg agcagactgc gcggaagagg ctgatcggca
     1261 agagatgctc tgcccggcat cgtgagagac aagaggctgt tgaagaaacc gatgacagag
     1321 acctcgagag tgcctgcaac cctgccggca acgcagccat gaaatcgtcg tcaaccgcgc
     1381 gcatgcgcac aacaaaggag gagttcgatg tccgtcgcca agattattga gattagttca
     1441 gaatcaccga aaagtttcga agaggccatc gtgcagggca tcgcccgggc atccaagacg
     1501 gtgcacaaca tcaaatcagc ctgggtgaaa gagcaacatg ttgtgatcaa caacggcaag
     1561 gttgacatgt atcgcgtgga cttgaaggtg acgtttgtgt tggattgacg agccgcgagc
     1621 gtcatggtcc tgataccact ggttgttcct gaagagctgc gtggatcggc gccccgatct
     1681 gatcgcggtg tggtgcactg tcctgggttg agattgcgcc gtttccagaa ggaatcatgc
     1741 gggtcttgga ttgtcggaaa cgtggtgatc gccctgatcc tggtttgctc cgccgttgtg
     1801 aacgcagcca gccagtcctc cgaatctccg accgaagtcg ttcgtgcgac gatcaccgag
     1861 gtcttccgca ttctcgaaga ccccaagctg aaaggcccgg cgatggtgat gccgcgtcgt
     1921 cgtatgttag aagaagtgat tgcatcccgc ttcgattatg cggagatgtc gaaacgggca
     1981 ctcgccgctc actgggcgcc gctgaccgca gctcagcgtg gcgaattcgt cgagctcttc
     2041 aagagttttc tctccgaccg ctatgccgaa aagattgaag ggtataacgg ggaacaggtc
     2101 cactacctgt ccgagcggat cgagggtaat tatgcggagg tacggaccga gattcgttcg
     2161 agcaaagtgg agatcccgat ggattatcgc ctgttcttaa aggagaccca atggcatgcc
     2221 tatgacatcg tcgttgacgg catcagcctc gtaaaaaact atcgaagtca attcgagaag
     2281 atcattcgaa ctgactccta tcaggaacta ctccgacggt tgcgggatcg cgcagtgacc
     2341 gaggaacgga aagggcagcg gtcgtagacc ccaggctttt tgcttggggc tagttgacca
     2401 gcggggaatg cgcgctatct tataagcgag cggaggcctc ggtgagagga acgctcaggg
     2461 aaaccgaggt cgagcgtagg ggcggtgagc aggcccacca agggtgggaa gcctaaaact
     2521 cctccaagac ctccaccatt cgagcgaagc tctctggacc cgcctccgct ccggtttttt
     2581 tctaccatct cccccttctc ctctacatat tgggtatgtt ctttgcaaaa tctattcgtg
     2641 gtggcttcat catgtggatg accatggggg cgtcattggt gtgtcatatg atggcggaca
     2701 ggatgagaaa agacgcgatg gcagggtggc cacataaaaa aacggcccgc cctttcgtgg
     2761 aacaccggac
//
LOCUS       DATIFR010000246         5226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_172233, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000246 DATIFR010000000
VERSION     DATIFR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172233"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /gene="groEL"
                     /locus_tag="VLD60_14035"
     CDS             <1..861
                     /gene="groEL"
                     /locus_tag="VLD60_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSB46130.1"
                     /translation="ATLVVNKLRGTLNVAAVKAPGFGDRRKAMLEDIAILTGGQVISE
                     DLGIKLENVKLTDLGRAKRVTIDKDNSTIVEGYGDPKKIEARVKQIKAQIEETTSDYD
                     REKLQERLAKIVGGVAVINVGAATETEMKEKKARVEDALHATKAAVEEGIVPGGGTAY
                     LRCISALDSLKLLPEQQVGVNIIRRALEEPIRQIVINAGMEGSVVVEKVRNDKNLSAG
                     YNAATEEYVDMIKAGIIDPTKVSRCALQNAASVAGLMLTTEVMITDIPEEKKEPMGGA
                     GHSHGMEGMY"
     gene            complement(1038..1793)
                     /gene="traT"
                     /locus_tag="VLD60_14040"
     CDS             complement(1038..1793)
                     /gene="traT"
                     /locus_tag="VLD60_14040"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HSB46131.1"
                     /translation="MLVLSLVIFSGSLSACRDMGQMMGDRNAELQTGQWWAGPIPDAA
                     PVKPEDRTAWISYKNVTQEQSYDLRGKLREGLISQGYKIIDDPDQANFQVFYTLRFFG
                     ENPSLDGGKNTSAGLGALGGGVGAAANMGRGRNAQQPNPIIANYSQVVEYNIIMDVRI
                     AQRKKGTVTHTLSGVQRDRQLSAAGGSTAGGVVAGGRATGMDNRQQLQETDNMLWQEN
                     RLVLWARQIRLTPGEAKPKLESAMANALPQLLP"
     gene            2632..2988
                     /locus_tag="VLD60_14045"
     CDS             2632..2988
                     /locus_tag="VLD60_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46132.1"
                     /translation="MIVEVESNENCESSFFARFKEAGSARPVTQVRLYERNPAGEWYW
                     VTGWSDNAETPACQAHAQLVEDSGSGLAHLVYGGIYGLRFKPITIEEPWNLASPNQWG
                     EAYLSLSSERDLRYAE"
     gene            complement(3053..4783)
                     /locus_tag="VLD60_14050"
     CDS             complement(3053..4783)
                     /locus_tag="VLD60_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46133.1"
                     /translation="MGVKKVIKRSSARGPLPGTPTLTRPDPTPVLLAAERSAEQVREL
                     LSVYGLQEIDQADRNLQAMAGDPHERHQLAYILPLLMESIARTADPDQALNHWERLLS
                     NVTRSSFLDYLSSSPRMLDLVCTIFGNSDSLAFTIIRDPMLVYWLAEQEVLSKPPTRT
                     GIEQLLYKQLARLTVKELKLEVLRRFRRREMLRIGVRDLLRLADVPETTASLSDLAGL
                     LIHAAYEIVDADLRRQYGVPMHKNRQGKWVQTAFAVIGMGKLGGQELNYSSDVDLIYV
                     YESHEGETRLPRGRQAEKPAAAGISNEEYFEILSRELTKALVEQTKEGYVFRVDLRLR
                     AEGTIGQLARSLDDYEKYYFTRGQVWERLALLKAWPVAGSMEVGQAFLKLIKPFIFGT
                     GVKRMDLARALAIVDDVRAVKEMIDAKMADRGHEGRNVKLGTGGIREIEFLVQTIQVL
                     AGKKIPALLDRSTIGALKRFAERKLISAKERDALTAAYLFLRDVEHKLQMVHELQTHA
                     LPHETEELQRCAVRMGYKSVDRVNALKRFQADHQRHTRYVNQMFRSLFYEGKNWPLLK
                     ATLRAINVLR"
     gene            5025..>5226
                     /locus_tag="VLD60_14055"
     CDS             5025..>5226
                     /locus_tag="VLD60_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46134.1"
                     /translation="MSTLAPNRDEILAALAPFCSDVPPDILQDFVGRMDQEYFRRFHP
                     ETIARHVHLAALLTPDQPCKLAI"
BASE COUNT         1180 a         1536 c         1417 g         1093 t
ORIGIN      
        1 gcgaccctcg tggtcaacaa gctgcgcggc accttgaacg tggcggccgt gaaggccccg
       61 ggcttcggcg atcgccgcaa ggccatgctg gaggacatcg cgatcctcac cggcggccag
      121 gtgatctcgg aagacctcgg catcaagctc gaaaacgtga agttgaccga cctcggccgt
      181 gcgaagcgcg tgacgatcga caaggacaac agcacaatcg ttgagggcta cggcgacccg
      241 aagaagatcg aagcccgcgt gaagcagatc aaggcccaga tcgaagagac cacctcggac
      301 tacgaccgcg agaagctcca ggagcggctc gccaagatcg tcggtggcgt ggccgtcatc
      361 aacgtcggcg ccgcgaccga gaccgaaatg aaggaaaaga aggcccgcgt tgaggacgcc
      421 ctccacgcga caaaggcagc cgtcgaagag ggcatcgtcc ctggcggcgg aacggcttat
      481 ctccgctgca tcagcgcgtt ggattcgctc aagctgttgc ccgagcaaca ggtaggtgtc
      541 aacatcatcc gccgggcgct tgaagagccg attcgtcaga tcgtgatcaa cgccggaatg
      601 gaaggctccg tggtcgtcga gaaagtgcgc aatgacaaga acctcagcgc cggttacaac
      661 gcggcgacgg aagagtatgt ggacatgatc aaggccggta tcatcgaccc gaccaaggta
      721 tcgcgctgcg cattgcagaa cgcggcctcc gtggcgggct tgatgctcac caccgaagtc
      781 atgatcaccg acatcccgga agagaagaaa gagccgatgg gcggtgccgg tcacagccac
      841 ggcatggaag ggatgtacta agataagtcg ctaggaagcg gtcagctttc agcaacgaag
      901 ggcccggcgg agatcccgcc gggcccttcg tatttctggg ccctggtaca accacaaggg
      961 caggtcaatc tctgtgttaa aacaggctcg cgcacttgtt atgcccccaa gaatgcctga
     1021 ccacttttct ccattcctta tggcaaaagt tgaggcaacg cattcgccat ggcgctttcc
     1081 agcttcggtt ttgcttctcc tggggtcagg cgaatctgcc ttgcccagag gacgagccgg
     1141 ttctcttgcc acagcatgtt atctgtttct tgcagttgct gcctgttatc cattccagtc
     1201 gctcgtccgc cagcaacaac accaccggct gtacttcccc cagcagcact cagctggcgg
     1261 tctcgctgta ctcctgataa cgtatgtgtg acggtccctt ttttccgttg cgcaatacgc
     1321 acatccataa tgatgttgta ctcaacaact tgactgtaat tggctattat aggattgggt
     1381 tgttgcgcat tgcggcctcg tcccatatta gccgcagcac caacaccacc accgagagca
     1441 cctaagcctg ctgatgtatt cttacctccg tccagacttg gattctcacc aaaaaaacgg
     1501 agcgtataaa agacttgaaa atttgcctgg tcgggatcgt caatgatctt atacccctga
     1561 gaaataagac cttcacgcaa tttgccacgc aaatcgtagc tttgctcctg ggtgacattc
     1621 ttgtaggaaa tccacgccgt acgatcttcc ggcttaaccg gggcggcatc cggtattggg
     1681 cctgcccacc actgtcctgt ttgcaattcg gcattacggt ctcccatcat ctggcccata
     1741 tcccggcaag cactcaggct tccgctgaat atcactaaac tgagcactag catgaggttg
     1801 ttgtatcttc gcatttctcc ccccttgttg gtgtttggtg ataccggcga actgtttctc
     1861 cgccgttcct gatataaact ccggaccgtt gatcaatacc gagaatcata tggctgcatg
     1921 tgccgcatcc ggatgatcga gaacactaaa cagagcgatc aatgccacga ggaggcctga
     1981 ggaaaggatc aaggatcagt gactgatcgc agaacggata ttgacccgga agaggaaggc
     2041 tgaccgttgg tgtgaggcga tgcacgacta cctctctgag cttcttcact caagaagcta
     2101 caacggtcat caattgtgaa gagcgtttcg cggagcgaaa agtgcgccac tatatctgta
     2161 agtgctccat ctacgcaata gagcctaatg gccttactcg tctggtgcac aaaaatacag
     2221 ctatttcgct gagactcgct aacacctgca cacatgagca aacgctgcac cagaacaatc
     2281 gcccaccgtg cgccgatctt ttcaattcga ctatttgagt gaataggccc ccactttatc
     2341 gcggtgagcc attgatgcag aagacaatca ctcgtccaat ggattcgagc ccttgcacct
     2401 atcagcgttc cctgtttttc ctaagaggac gacccgatgt tctgtctcga ctacggtggg
     2461 atttgttgcg cttcctcgaa gagaatcagt agagtcaaca gcagggagaa gacgaagaag
     2521 ccacagcgtg aggcagaatt gacgctcagt ggtgaaggag caacctcccc aagtgctcct
     2581 ctgggtatac gaatgcgacg cgtctgatct ggaattcggg aagaaacgcc aatgatcgtc
     2641 gaagttgaat caaacgagaa ttgcgaatcg agcttttttg cgcggttcaa ggaagccggg
     2701 tcggctcgac cggtcacaca ggtccgcctc tatgaacgaa atcccgccgg cgaatggtat
     2761 tgggtcacag gatggagcga taacgcagag acaccggcat gccaggccca cgcccaactg
     2821 gtcgaagact caggatcagg gttggcgcat ctggtgtatg gaggaatcta cggcctgcgg
     2881 ttcaagccga tcacaatcga ggagccgtgg aatctcgcca gcccgaatca gtggggagaa
     2941 gcctatctgt ccctctccag cgagcgcgat ctgcgatacg cagaatagcg ttctgtgggg
     3001 gcattgaaga tctgacggcg tatagagatg gctcggagca cggacaagcg gctcaacgga
     3061 gaacgttgat ggctcgcagt gtcgccttca ggagcggcca gttcttcccc tcatagaaga
     3121 gcgaccggaa catttgattg acgtaacggg tatgtcgctg atggtctgct tgaaaccgtt
     3181 tgagggcatt gacgcgatcc acgcttttgt aacccatgcg gacggcgcag cgctgaagct
     3241 cctctgtctc atgcggcaac gcatgggtct ggagttcgtg caccatctgc agcttgtgtt
     3301 cgacatcccg aaggaataga taggccgctg tgagggcatc ccgctctttc gctgaaatca
     3361 atttccgttc ggcaaaccgc ttcaacgcac cgatcgtgct gcgatcgagg agcgccggga
     3421 ttttcttgcc ggccagcact tgaatcgtct gcacgagaaa ctcaatctcc cggatcccgc
     3481 cggtaccgag cttcacgttc cgcccctcat ggccccgatc ggccatcttc gcatcaatca
     3541 tttccttcac tgcccgcaca tcatccacga tcgctagcgc gcgggccaga tccatccgtt
     3601 tcacgcccgt gccgaaaata aacggcttga tgagcttcag gaacgcctgc ccgacttcca
     3661 tggagccggc caccggccag gccttaagca aggccaatcg ctcccagacc tgccccctgg
     3721 tgaaataata tttttcatag tcgtccagag aacgagccag ctgaccgatc gtgccttccg
     3781 cccgcagacg caaatccacg cggaagacat agccctcctt cgtttgctcg actaatgctt
     3841 tcgtcaattc ccgcgagaga atttcgaaat attcttcatt cgagattccc gcagccgccg
     3901 gcttctctgc ttgtctgccg cgcgggagcc tggtttcccc ttcgtgtgat tcatagacgt
     3961 aaatcagatc cacgtcggaa ctataattca gctcctgccc gcccagcttc cccattccga
     4021 tgacggcgaa ggcagtctgc acccacttcc cctgccggtt cttgtgcatg gggacgccgt
     4081 attgccggcg caagtccgca tccacaatct cataggctgc gtggatgaga agacccgcca
     4141 gatccgacag cgaggccgtc gtctccggaa cgtccgccag ccgcaagaga tcgcgcacgc
     4201 cgatccgcaa catctcccgc cggcgaaaac ggcgcaggac ttccagcttg agttccttga
     4261 ccgtgagccg ggccagttgt ttgtacagta actgttcgat gccagttcgt gtcggtggtt
     4321 ttgacaggac ttcctgctcc gccaaccaat agaccagcat cggatcccgg atgatcgtga
     4381 atgccaggga gtcgctattg ccgaaaatgg tacagaccag gtcaagcatg cgaggcgaac
     4441 tgctcagata atcgaggaat gacgaccgcg tgacattcga gagcagacgt tcccagtggt
     4501 tcaacgcctg atcgggatca gcggtccggg caatcgactc catcagtagc ggcaggatgt
     4561 aggccaactg atgacgctca tggggatcgc ccgccatggc ttgaagattg cggtcggcct
     4621 ggtcgatttc ctgcaagccg tacaccgaca gaagctcccg cacctgttcg gcgcttcgct
     4681 ctgctgcgag caaaaccggt gtagggtccg gcctcgtcaa cgtcggcgtc ccaggcagcg
     4741 gcccgcgtgc tgaagaccgc ttgattactt ttttaacacc caagaaagtc tcctaagtga
     4801 aggggtacgg aaaacatctc accatccgca ggctgttcaa aatggccgcc agcaaggccg
     4861 cagcgagcga ggaggcgagg cgtacttttt actgtacgtt gagcctttga gcgaagcgag
     4921 aacgcagctg gtggactttt tcaacagcct gctagtcatg gcgcggcatt atactgacag
     4981 gctcccctac gcacaatgaa ggcgttccgg tatacttggc gtccatgtct accctggcac
     5041 cgaaccgcga cgagatcctc gcggccctcg caccgttctg ctccgacgta ccgcccgata
     5101 tcctgcagga cttcgtcggg cgcatggacc aggaatactt ccggcgattc catccggaga
     5161 ccattgcgcg gcacgtccac ctggccgccc tcttgacacc ggatcagccc tgcaaactcg
     5221 ccatca
//
LOCUS       DATIFR010000247        18790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1325463, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000247 DATIFR010000000
VERSION     DATIFR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18790
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325463"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VLD60_14060"
     CDS             complement(<1..551)
                     /locus_tag="VLD60_14060"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSB46135.1"
                     /translation="MKTWQRSLLALCALLALFGGAAYAQAPGAPPVEFPYTGNRTAVW
                     IVAQLHILFAGFILGAPIFVVISEWLGYRKQDPRYDRLAKEVTKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLVFAVVYPLLFIGETILLYMYFYTWDAWKGDKKARH
                     IALGVLLNLIGTITLFVIDGPTS"
     gene            complement(608..1117)
                     /locus_tag="VLD60_14065"
     CDS             complement(608..1117)
                     /locus_tag="VLD60_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB46136.1"
                     /translation="MKQTTFAQLAVLLGLGLAVVACGETGEGPIVPPPPAPGEYADKH
                     MPAGWWTDAAKVEEGRKIFIGETNPDVNCASCHGKDGKPVKAGARDFRNGERMKLYSD
                     SVWFWRISEGVPNTKMKAWKSKLSDEDRWKLVLFERNFGLAGKAWDAEKKDWADAGNI
                     AVAQAEAGK"
     gene            complement(1300..1833)
                     /locus_tag="VLD60_14070"
     CDS             complement(1300..1833)
                     /locus_tag="VLD60_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46137.1"
                     /translation="MRYAKQRQTGLWVALAACALMAGFPSGVSATHEADHRFTVEGFV
                     CGADGRGVSESDVLVKDTKISYGQIVKTEGDGYYKATFHLHNDNLGDPLLVEAKGEQQ
                     NLKVQFDPKDLESERKIQVNFGTGCVHDRSSPPGWLWWGLGAVATAAVGVIGLKMVRS
                     QRKLEQKRGKSQGKRKQ"
     gene            complement(2080..2475)
                     /locus_tag="VLD60_14075"
     CDS             complement(2080..2475)
                     /locus_tag="VLD60_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46138.1"
                     /translation="MTDTKNHTSGNGAGSLEGRIFQPASPAELAEAIELAFDYRGDVT
                     IGLRTGESVTGYLFNRQAAGTDPYIEVFPADNPAPRRIAYAVILSIAFTGEDTANGKS
                     WEAWVSKKESERRTEAARVESDARTRGYL"
     gene            complement(2501..3517)
                     /gene="hpnH"
                     /locus_tag="VLD60_14080"
     CDS             complement(2501..3517)
                     /gene="hpnH"
                     /locus_tag="VLD60_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HSB46139.1"
                     /translation="MAVPISQMLTVSKYVLAQKFKGVKRYPLVMMLEPLFRCNLACAG
                     CGKIQYPDHVLDKRLTPEQCWAAAEECGAPIVSIPGGEPLIHPEMPKIVEGLISQRRY
                     VYLCTNAILLERKLDEYKPSKFLTFSVHMDGLKDEHDLAVCRDGVYDVAVKAIKAALK
                     RGHRVTTNTTLFDDANPERVRKFFDQMMELGVEGMMISPGYSYQKAPDRQHFLKRERT
                     RELFSKILSSPKRGWQFNQSPLFLDFLMGRRDYQCTPWGNPTYNVFGWQKPCYLLQEG
                     YTKTFRELMELTEWDHYGTGRNEKCADCMVHCGYEASAVEDTFGSVSGFSRTVKLTLL
                     PTSR"
     gene            complement(3668..3742)
                     /locus_tag="VLD60_14085"
     tRNA            complement(3668..3742)
                     /locus_tag="VLD60_14085"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3708..3710),aa:Cys,seq:gca)
     gene            complement(3818..4999)
                     /gene="ispG"
                     /locus_tag="VLD60_14090"
                     /gene_synonym="gcpE"
     CDS             complement(3818..4999)
                     /gene="ispG"
                     /locus_tag="VLD60_14090"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSB46140.1"
                     /translation="MHITRRKTRQITVGKLKIGGDAPVSVQSMCSTDTRDVAATVEQI
                     RQLETAGCEVIRVAVPDEEAAAALSKIKSEMTVPLIADIHFDHRLALKAAEIVDCVRI
                     NPGNIGAWWKVEEVIKAVNDRGIPIRVGVNGGSLERPLLDKYGWPSPEALSESALNAV
                     HALEDVGFTNMKVSLKASDVHHAIDAYWLFAHQSDYPLHIGITEAGTAMTGAVKSAMG
                     LGYLLAQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKAPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PMEELMDTLIQEVEQLAKEKEAEGNGEAVAPAGEGWEPLVPESAQPSTLGKKIPVLPN
                     R"
     gene            complement(5048..6997)
                     /gene="dxs"
                     /locus_tag="VLD60_14095"
     CDS             complement(5048..6997)
                     /gene="dxs"
                     /locus_tag="VLD60_14095"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSB46141.1"
                     /translation="MSILKTIQSPADLKRLSPAQFPMLCQELREQIIGVVSNVGGHLA
                     SNLGVVELTVALHYLLDTPKDKIVWDTSNQAYAHKLLTGRREQFHTLRQFGGMSGFCK
                     REESEYDTFNAGHAGTGVSAAYGMVEAREQLKRKNKVVCVVGDGAMTAGMTLEGLHHA
                     GGLGKDFLVILNDNQMSISKNVGAISSYLNRTFTGDFYTKMREETGQLLRKIPHIGSD
                     MEKLARRAEELAKGAILPGLLFEELGFRYAGPIDGHNFEHLLPALENVLKMKGPVLLH
                     VITKKGLGYEPAVKNPVWFHACPPFVRETGSPAKKAARPSYTQIAVESLVKLAREDKR
                     IVAITAAMCEGTGLNIFEKEFPERLYDVGIAEQHAVTFAAGLATQGMRPVVAMYATFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDYAYLRHMPNMTVMAPKDE
                     NELQHMLKTCLAHDGPASVRYPRGVSLGVNMDETPKALPLGKGELMREGTDVAIIAIG
                     VTVWQAMKAAEKLSQEGISAAVVNARFVKPLDQNLIVDVAKRVRYVVTVEEGCKMGGF
                     GSAVLEALSDAGVHDVTTKVLGLPDWYIEQGPQDLLRERYGLTAEGIFESVKELVGKA
                     PATPREKLTVSVMGDRLPHGDEQGS"
     gene            complement(7154..8188)
                     /locus_tag="VLD60_14100"
     CDS             complement(7154..8188)
                     /locus_tag="VLD60_14100"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HSB46142.1"
                     /translation="MSRKIGYPNRIVRLLAGALTVLALSSSVAWAEGFGPFPVRNFQA
                     FQQLVLNLPGDRASVLKQGTLDLRLELAETASIYNEIQPQATAVIKFETLRSGLFLRY
                     GLTEKVELGVELPVYYRYQGFMNGAITATERATTGEAPARAALSNTSYAFNVTRNGQT
                     VMSGGPGATGLGDTTLMSKFQFLTESGSVPAVSLRGAVKLPTGDQSAFFGSGSPDFGA
                     GLAVEKHLASRWILYGNINGVVPTGSIAGFSLQPTFTAIVAVEYLWSENLSITAHFDY
                     YSSPFHGTGLSMLKSDVTESVLGFSYRVAPHLLWQFYAVENVDFVTGSAADVTISTLL
                     TYRFESNSGR"
     gene            complement(8196..9398)
                     /locus_tag="VLD60_14105"
     CDS             complement(8196..9398)
                     /locus_tag="VLD60_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSB46143.1"
                     /translation="MTTLHGIVVFTVCLLVGFGMAWVESACADTQVFPIPSASTSKND
                     GNDAGLIVPILITDPDGELKYLLAPMIIRNSIVGTRGTFNLFRYDTGGRQMQFIASYT
                     EHIERKLVFNYTDPAFSNGRYFLNFGGSFFKNATARFFGLGQETQESSETNYTSREAR
                     ANWRFGVYANEVTQVAVSQRFRQVSLQRGGTDLPFTGEVFPTVDGVSGETIIVGHRAT
                     FYYDTRNNLSSPTDGMAVTAYAEVNQNVHNGDHPVYSRYELEVKKLFPSESKRAILVL
                     RADLQATLGDQVPFFEQSSLGGQNNLRGYGVDRYIDKHLIAASIEERIHVARAKVAGV
                     AADFEIAPFVDTGQVFNNFKDVSLKDYRITPGIGFRGIVRPNVVGRIDLGFSKEGSAV
                     FAGLDFPY"
     gene            complement(9419..10060)
                     /locus_tag="VLD60_14110"
     CDS             complement(9419..10060)
                     /locus_tag="VLD60_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB46144.1"
                     /translation="MTCAVAGKRGIGRAICMSIVLSLLLCNGLTAGNVWAGGATDAMK
                     ATIDEVLRIIQDKDLKQPGKSEERRMLLEKVVGERFDYQEMSRRALGAPWANLSAKER
                     DEFVVLFQTLLVNSYADKIESYSGEGVQYLNERTEKDYAEVRTKVLTGKTEIPLDYRL
                     LNKASVWRVYDVVVDGVSLVNNYRGQFTKILRTGTYVDLIDQLRKKSDKIKTP"
     gene            complement(10118..10561)
                     /gene="mlaD"
                     /locus_tag="VLD60_14115"
     CDS             complement(10118..10561)
                     /gene="mlaD"
                     /locus_tag="VLD60_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSB46145.1"
                     /translation="MEKAKLELIVGVFVLVGILCLGYLSIKLGKLELIGGNVYEVVAQ
                     FTSASGLKPGSAIEIAGVEVGRVRAIVLKEDRAAVTLAVDDSVKLYTDTIASIKTRGI
                     IGEKFLALSPGGGGDPLKPGDTIRDTESGLDLEELVSQYVHGKVN"
     gene            complement(10566..11345)
                     /locus_tag="VLD60_14120"
     CDS             complement(10566..11345)
                     /locus_tag="VLD60_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB46146.1"
                     /translation="MLNAMIKLVGVEKTLGGQRVLQGVDLSIPAGKLTTVIGKSGEGK
                     SVLLKHMIGLMQPDRGEVWVGDVEISRLRGTRLNDVRKRFAMLFQGAALFDSLSVSEN
                     VAFPLREKLRMKGPEVMRRVEEKLAEVGLAGMGHKFPAELSGGMRKRAGLARALVMEP
                     EIMLFDEPTTGLDPLMAKSIHDLIVGMQRRFKFTAVMVSHEIPEIFAISDWVAMLRKG
                     KIAAMAPAAEFVKTTDPEIHEFISVSGPVTLPGTPVANQEG"
     gene            complement(11400..12161)
                     /locus_tag="VLD60_14125"
     CDS             complement(11400..12161)
                     /locus_tag="VLD60_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HSB46147.1"
                     /translation="MIERVGARVIALIQEMGRMLLFVLSSFAWLTRPPFRLTQIVKQL
                     HFIGYKSTFVVVLTAGFTGMVLALQGYYTLRKFGSEGLLGSAVALSMIRELGPVLAAL
                     MVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIAPKLVAGLIGVPLLVAIFDV
                     VGIYGGYLVGVDLLGGNGGAYWNSIESAVEWKDVYGGILKSISFGLIVSWICCYKGFF
                     TRQSAEGLGTATTEAVVLSSVLILVWDYFLTSVLL"
     gene            complement(12176..13021)
                     /locus_tag="VLD60_14130"
     CDS             complement(12176..13021)
                     /locus_tag="VLD60_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46148.1"
                     /translation="MLSVWSLTQQPTSSRDARQFHAESQVNPVAMFVATRWELAALRR
                     ALPVDRRQHVGGIRCLIGRQGNRPYWLIQTGVGSDAASTAARSVLNRQPMALIISTGF
                     AGALTPAAIGDLVIGTSVCAGSYDRTWRQNTEAVSCDHAGLSCVQSVAAQVGIAAHLG
                     PVVSVPRIVYRTEDKQAISRMTGATALDMESAALALVAQERAVPFMVVRTVSDLVGEE
                     LPVDFNLFLKPAGWLRGMWELLASPSKLLGMNRLRQQSGLAADQLTAVHTAFARRGFG
                     LSQTL"
     gene            complement(13000..15177)
                     /gene="shc"
                     /locus_tag="VLD60_14135"
     CDS             complement(13000..15177)
                     /gene="shc"
                     /locus_tag="VLD60_14135"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSB46149.1"
                     /translation="MKLIRTILDRLSDRLFISVPQKMRLAADVSSQPPLRLVSDKSAA
                     LPTADSSTRRSASQMGSQLDAIDDAVRRSQAWFLARQHPAEGYWVAELEADTTLTSEY
                     LMLRRFLDRVDPERERKAVRYLRATQLPDGGWPIYYGGPSEISASVKAYFALKLSGIS
                     ADEPFMLRAQDCIRAHGGVVQSNVFTKIALALFDQYDWEGIPHMPVELMLLPKGFYFN
                     IYAISYWSRTVLIPLLIVFAHRPVCKLPKEQGIDELYLIPRNEIRYWKFPPFNKDQKW
                     FTPHNFFVALDAMLKLYDRMPLMWLREKALHRAATWMLEHIKGSGGLGAIYPAMANSI
                     MALQCLGYQVDDPLVQKALQEIEDLEVYDTISVGEQRVDALHLQPCHSPIWDTSLLMN
                     ALVESGMAQDHPALQRGAAYLMSRQTRAVGDWKCSSPHAEPGGWYFQHENELYPDVDD
                     SAVVLMALSKVKMGRTAELQDSIRRGAAWVVAMQGSDGGWGAYDKDNNRIVFNYIPFA
                     DHHALLDPSTADLTGRCLEMLAALGYDQTHPAVAPALAFLKHEQEPDGSWYGRWGVNY
                     IYGTWSVLAGLRAIGEDLSQPYIRRAVTWLESKQNPDGGWGESCLSYADAAASGRGES
                     APSQTAWALMGLMSAGMTDSFSVARGIQFLLRHQMKDGSWEEVRHTGTGFPRVFYLRY
                     HWYCQYFPLWALAMYRNLRTRNKMRADEVRHQILTAGCYRSGR"
     gene            complement(15374..>18790)
                     /gene="smc"
                     /locus_tag="VLD60_14140"
     CDS             complement(15374..>18790)
                     /gene="smc"
                     /locus_tag="VLD60_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HSB46150.1"
                     /translation="VDSQSGLPSQLSEYQDLMITRRLYRNGDSEYLINKTLCRLKDVR
                     SVLLDTRAGTKGHTVIAQGQIDQILNASPQDRRELIEETAGIIRYKKQKAEALRKLDA
                     TQQNLLRVRDIIAEVKKQLNSLERQARQARSYQVLQQEARALEIRLLTNEYRRLRSTI
                     ADTERELEGLFHEESERVAEQARFNAELERIKLAIAEAGSVIDQRRDEFSKIEQQQAQ
                     ALTAAEVERHRSEMYEEQRVQGAQELERLTQEQEEVKAAETALLVTLETLERELTERE
                     QVLAGLDQEMKSLLHQRAGAIQEEERGRLDVLNLAVLVANTEQSLSQLALRAEEVAER
                     DGRLAREREEIQSQHDSTREKRHALEQASQEAERVVVELREQQQQLGQKAEQVATGLT
                     DVDQRLLSQSEELAAVDSHLRALQGVLREDMGYGRSGDEESTALKTCEGVHEAVAEWL
                     VVPAGLDRAVEAILGERVRGWFVESPQTAARAIAFLMGKDLGRGTFIPESPRWTAQAA
                     GSADWWQAIAGQAGVIGRAVELVQVEGHRDAARDYLFDRVVFVESLQEAMQLWEQHTW
                     SANDGPTFVTRAGEVLDGAGVVTGGQVSSTGGLLQRRREVLQLESRRISLMESVEAER
                     QRREQLLAESQFLREEGRRVADALREGEMRELSLHKDVVGAQQLLGDLTQRIETLMGD
                     AQRGSLEVQRLEQETRSGHAQLAQWIAEKSGQEGALQAVRARLIQLDQGIQGLQQRLT
                     EAQLAVQDLRTTREHHGSDLQRIRQEKQEAVSRIETLSRQLESLRTSLEQSREERTRQ
                     ECVCRELGQSAEQVKSRLIEAQEGQAQDMMQAQEIETAVDAVRKTVSALRESRMSVEV
                     RRAEVRTQLGTVESTLSGTYQVDPMSLIEASVAGAAFATPQGEAESTVEVASTPTEDD
                     LRQQIQKIRERLDRMGPINLAAINEHQELEERWKFLSTQEQDLSTSIGSLKEIIQRIN
                     RTTKDMFAATFAELQEKFGEVFTKFFPGGRAELQLVEVPVDEDAEGKGPQEPGVEIVA
                     QPPGKRLKNITMLSGGEKTLTAMALLFASFLIRPTPFCILDEIDAPLDEENIGRFTAV
                     LCELAQGAQFMVITHNKRTMGVADSLFGVTMEEPGISKLVSVRLTDLQPV"
BASE COUNT         3971 a         5915 c         4862 g         4042 t
ORIGIN      
        1 aaggaggtcg gtccgtcgat cacgaacagc gtgatcgtgc cgatcaagtt gagcaataca
       61 cccaaggcga tgtggcgcgc cttcttgtcc cccttccatg catcccatgt atagaaatac
      121 atgtagagca ggatcgtctc gccgatgaac agcagcggat acacgacggc aaagaccagg
      181 aaaaagtgat tgatcagcca ggtggtgaat tgcggatagg tcgccagcaa cacgaagatg
      241 aacaggccgc ccgtgagggc ggtcatgctg tagagaatga ccgtgacttt cgtgacctct
      301 ttggccaagc gatcgtagcg gggatcctgc ttccggtagc ccagccactc cgagatgacg
      361 acgaagatcg gcgccccgag aatgaacccg gcaaacagga tgtgcagttg ggcgacgatc
      421 cagaccgcgg tccgattgcc cgtgtagggg aattcaaccg gaggcgcccc tggagcttgc
      481 gcataggccg ctcctccgaa cagcgccaga agcgcacaca gagccaagag actgcgctgc
      541 catgttttca tgatattccc cgccctcctc cactaaatcc cggttcgacc ggccccggtt
      601 ccgccgctta tttcccagcc tcggcctgtg ccactgcgat gttccccgcg tccgcccaat
      661 ccttcttttc tgcgtcccac gccttgccgg ccagaccgaa gttccgctcg aacagcacga
      721 gcttccagcg atcttcatcc gacaacttgc tcttccaggc cttcatcttc gtattgggca
      781 cgccctcgga gatacgccag aaccagacgg agtccgagta gagcttcatc cgttcgccgt
      841 tgcggaaatc ccgcgcgccg gccttgacag gcttgccgtc cttaccatgg cagcttgcac
      901 aattcacatc cgggttcgtt tcgccgatga aaatcttccg accttcttcc accttggccg
      961 catccgtcca ccagccggcc ggcatatgct tgtcggcata ctcgccggga gccggaggag
     1021 gcggaacaat cggaccctcc cctgtttcac cgcaagcaac cacggctaaa cccaaaccta
     1081 gtagcaccgc cagctgtgca aaagtcgtct gtttcataac gatccctcgc tcctttatat
     1141 agaattcaag acgaaaaaaa gacgctttga ccggagcgaa ttcactcccg caaaagcgcc
     1201 gataccctcg agattccctg aagccacgcg cactgcccct cgccacatcc attgaccgat
     1261 ccggcgaggt gcacatcagc cccacggacc gcccagtgat tattgcttgc gtttcccctg
     1321 cgacttgcct cgtttctgtt ccagcttccg ctgcgaacga accatcttca gcccgatcac
     1381 cccgaccgcc gctgttgcta cggctcccag cccccaccac agccagcccg gaggactact
     1441 cctatcgtgg acacagccgg tcccgaagtt aacctgaatc ttgcgctcgg attccaggtc
     1501 tttcggatcg aactgaacct tcagattctg ctgctctccc tttgcctcca ccaagagcgg
     1561 gtcgcccaga ttatcgttgt gcaggtggaa cgtggcctta tagtacccgt ccccctcggt
     1621 cttgacgatc tgcccgtagg aaatcttggt gtccttgaca aggacgtcgc tttccgaaac
     1681 ccctctcccg tccgcaccgc acacgaaccc ttcgacagtg aagcgatgat ccgcttcgtg
     1741 cgttgccgag actccggatg gaaatcctgc catcagcgca caggcagcca gcgcaaccca
     1801 caatcctgtc tgtctctgct tcgcgtatct caaactggat ggcgtccacg ccgctcgatt
     1861 ccgtgcaatg gtttcaaaca acgaaacgcc tcgccacgac gggcttcccc agttttgagg
     1921 ccgatgtttc taacataggc ccccctggtt gtcaacgaca agacaaggcc tctaaaggga
     1981 tgcccctgtt ctcgcgaata gcacttgacg ctgaggagaa tattgtgatt tgggggggag
     2041 tgaaaaagag aaggatctga aacttcaaac tgaaatattc tacaaatatc cgcgggtgcg
     2101 cgcatccgat tcgacccgcg cggcctctgt ccgccgctcc gactctttct tcgaaaccca
     2161 ggcctcccac gacttgccat tcgccgtgtc ttctccggtg aacgcaattg agaggatgac
     2221 ggcataagca atccgtcgag gcgccgggtt atcggcagga aacacttcga tgtagggatc
     2281 agtaccggcg gcctgccggt taaacagata gcccgtgacc gactcgcctg tgcgcaggcc
     2341 gatcgtcacg tctccccgat aatcaaaggc gagctcaatc gcctcggcta attcggccgg
     2401 agaggccggc tgaaaaatcc gcccttcgag cgaaccggca ccgttgccac tggtatggtt
     2461 ctttgtatcc gtcatggtag taaggtgtgg atggccggcc ctatcggctg gtcggcagca
     2521 aggttaattt gaccgtccgg ctgaaaccgg agacgctacc gaatgtatct tccacggcag
     2581 aggcctcata gccacagtgc accatacagt cggcgcattt ctcgttccgg cccgtgccgt
     2641 agtggtccca ttccgtcaac tccatgagtt cacggaacgt tttcgtatag ccttcctgca
     2701 acagatagca gggtttctgc cagccgaaga cgttatacgt gggattgccc cagggcgtgc
     2761 attgatagtc ccgtcgaccc atcaaaaagt cgaggaataa cggcgactga ttgaattgcc
     2821 agccccgctt cggactactg agaatcttcg agaacagctc ccgcgtccgc tcgcgtttca
     2881 agaaatgctg ccggtccgga gccttttgat agctgtagcc aggggaaatc atcattcctt
     2941 cgacacccaa ctccatcatt tggtcgaaga atttccggac acgctcgggg ttcgcatcgt
     3001 caaagagggt ggtattggtc gtcacccgat gcccccgttt cagggccgcc ttaatcgcct
     3061 tcaccgccac gtcatagact ccgtcgcgac agacggcaag atcatgctca tctttcagac
     3121 cgtccatgtg cacgctgaag gtcaagaatt tcgacggctt gtattcgtca agtttgcgct
     3181 ccagtaagat cgcatttgtg cagagataga cgtatcgccg ctgggatatg aggccctcga
     3241 caatcttggg catttcggga tggatcaagg gctccccgcc gggaatgctc acgataggag
     3301 ctccgcactc ttccgcggcc gcccagcact gctcaggtgt caggcgctta tcgaggacat
     3361 gatccggata ttggatcttc ccgcaaccag cgcaggccaa attgcagcgg aagaggggtt
     3421 ccagcatcat gactaacgga taacgcttca ctcccttgaa cttctgagca agcacatact
     3481 tggacactgt aagcatctgg gaaatcggaa cggccattcc gctctccttc tttaaacttt
     3541 tgtagcgtga tcgcctgcga atcgcagtcg gactaggagc ttataaacgc tgaaattcta
     3601 acatcctgcg gacacagcgt caatttcatg attctcgccc ttccgaggtc ataccaacgg
     3661 aacggtttgg aggcgccgag cgggttcgaa ccgctgcatc gcagttttgc agactgctcc
     3721 cttaccactt gggtacggcg cctcgcgagc gcggcattat agcggcccgc gttccatgat
     3781 ctcaactcac actttctgga ataggcttcg gagactctca gcggttgggc aacaccggga
     3841 ttttcttccc cagtgtcgac ggctgagcgg attcgggcac gaggggttcc catccttcac
     3901 cggcaggagc gacagcttcg ccatttcctt cggcttcttt ctctttggcg agctgttcca
     3961 cttcctgtat caacgtatcc atcaactctt ccatcggcac tttgcggacg agtttgcctt
     4021 tcttgaacag gatgcctttg ccttcgccac cggcaatccc gatatccgct tctttccctt
     4081 cgccgatgcc attcacaacg cagccaagaa ccgacacgtt cagcggtgcc ttgatgtggc
     4141 cgagcttctt ttccaactca ttggccatgc ggacgacatc gatttcgact cgtccacagg
     4201 tgggacacgc aataacgttg atgccgcggt gacggagttc gagcgacttc agaatctcaa
     4261 agccgacttt gacttcttcg accggatcag ccgccagcga aacacgcaac gtatcgccga
     4321 taccttgagc caacagatac ccgagcccca tggccgattt cacggccccg gtcatggcgg
     4381 tgcctgcttc ggtaatgccg atgtgcaacg gatagtcgga ttgatgcgca aagagccaat
     4441 acgcatcgat tgcatggtgt acgtcggatg ccttcaacga cactttcatg ttcgtgaagc
     4501 cgacgtcttc gagcgcatgc acggcattca gtgcagactc cgaaagggct tcaggcgaag
     4561 gccagccgta cttgtccagc agtggccgtt cgagcgaacc gccgttcaca ccgactcgaa
     4621 tcggtatccc acgatcgttc acagccttga tcacttcttc gactttccac caggcgccga
     4681 tattgccggg attgatccgg acacagtcga caatctcggc ggctttcaaa gccagtcgat
     4741 ggtcgaagtg aatgtccgca atcagcggca cggtcatctc ggatttgatc ttggagagcg
     4801 cagcggcagc ttcctcatcc ggcaccgcca cgcgaatcac ttcacagccg gccgtttcca
     4861 attggcggat ttgttcgacc gtcgcagcca catcgcgtgt atcggttgaa cacatcgatt
     4921 ggacggagac gggagcatca ccgccaatct tcagcttccc gaccgtaatt tgtcgcgtct
     4981 tgcgtctggt tatgtgcatc cgacgatacc cgccttaccc cttacgtctc acacctcacg
     5041 tcttcgatca gctaccctgc tcgtcgccgt gcgggagccg atcgcccatc actgataccg
     5101 tcaatttctc acgcggcgtc gcgggagcct tgccgaccaa ttccttcacg ctttcaaaga
     5161 tcccttcggc ggtcaaacca tagcgttcac gtaacaaatc ttgtggtccc tgttcgatat
     5221 accagtcagg gagacccaga accttggtcg tcacatcgtg cacaccggca tcagagagcg
     5281 cctcaagcac cgcggagccg aacccgccca tcttgcaacc ctcttcgacg gtcaccacgt
     5341 agcgaacgcg cttggcgaca tcgacgatca ggttctgatc caacggcttc acgaatcggg
     5401 cattcaccac cgctgccgaa attccctctt ggctcagctt ctcggctgcc ttcatggcct
     5461 gccacacggt gacgccgatc gcgataatgg ccacatcggt gccttcgcgc atcagttcgc
     5521 ccttgccgag aggcaaggcc ttcggcgtct cgtccatgtt cacgccaaga ctcacaccac
     5581 gcggatatcg cactgaggca ggaccatcgt gcgccagaca ggtcttgagc atgtgctgga
     5641 gttcgttttc gtctttcgga gccatcaccg tcatgttcgg catgtgtcgt agataggcat
     5701 agtcgaacgc cccatggtgc gtcgtgccgt cttccgcaac caagccgccg cggtcgatgc
     5761 agaacgtcac gggcagattc tgcgtcgcca catcgtgcac gacctggtcg tatgcgcgct
     5821 gcaggaacgt cgcatacatc gcgacgaccg gccgcatccc ttgcgtcgcc aacccggccg
     5881 caaaggtgac cgcatgttgc tcggcgatgc cgacatcata gagacgctcg ggaaattcct
     5941 tctcgaaaat attcaaaccc gtcccctcgc acatggccgc tgtgattgcg acaatccgct
     6001 tgtcttcgcg cgccagcttc acgagcgatt caactgcaat ctgcgtatag gaaggacgcg
     6061 cggctttttt cgccggcgag cccgtttccc gaacaaaagg cgggcaggca tggaaccaga
     6121 cgggattctt gaccgccggc tcatatccca gacctttttt cgtaatgacg tgtagcagaa
     6181 cggggccctt catcttcaac acgttctcaa gcgccggcag gagatgttcg aagttatgtc
     6241 cgtcgatcgg accggcgtag cggaatccaa gctcttcaaa gagcagcccg ggaagaatgg
     6301 cacccttcgc caattcttcg gcccgtcgag cgagtttctc catatcggat ccgatatgag
     6361 gaattttcct cagcaactgc ccggtctcct cccgcatctt tgtgtaaaaa tcgccggtaa
     6421 aggtccggtt caagtatgag gagatcgctc cgacgttctt tgaaatcgac atctggttgt
     6481 cgttcagtat caccaggaaa tccttgccga gccctccggc atgatgcaga ccctccaaag
     6541 tcataccggc ggtcatcgcg ccgtcaccga ccacgcagac gaccttattt ttccgtttta
     6601 attgttcgcg cgcttcaacc atgccgtagg ccgcagagac gccggttccg gcgtggcccg
     6661 cgttgaatgt gtcgtactcg ctctcttcgc gcttgcagaa gccgctcatt ccaccgaatt
     6721 ggcgcaaggt atggaactgc tcacgacggc cggtcagcag cttatgcgca taggcttggt
     6781 tacttgtatc ccagacgatt ttgtccttcg gggtatccag gaggtagtgc agcgcgaccg
     6841 tgagttcgac cacaccaaga ttcgaggcga gatgcccccc cacgttagac accacaccga
     6901 tgatttgctc tcgaagctct tggcacagca tgggaaattg tgccggcgag agacgtttca
     6961 aatcagcggg gctctgaatg gtcttcagga tcgacatagg agtcactcct ttgcgtgaat
     7021 tcatgtgccc ggtaataacg ggctgagttc ggacaggcct ggcgaagcag ttaagcggtt
     7081 ctacgctggc tcagagagtc tcacataaga ctcccatatt gtcaagcggt tatagccaac
     7141 tccaccggac tcatcagcgg ccggagttcg attcgaaccg gtaggtcagt aatgtcgaaa
     7201 tggtgacgtc tgcggcactg ccggttacga agtcgacatt ctcgaccgca taaaactgcc
     7261 acaacagatg cggggccacg cgatagctga accccagcac tgattcggta acgtcactct
     7321 tgagcatgga cagaccggtt ccatgaaacg gtgatgaata atagtcaaaa tgagccgtga
     7381 tcgacaggtt ttctgaccag agatactcga cggccacgat cgcggtgaag gtcggttgca
     7441 gtgaaaaccc cgcgatactg ccggtgggaa cgacgccgtt gatgttgccg tataggatcc
     7501 atcgactggc cagatgcttc tcaactgcca ggcccgctcc aaagtccggg cttccgcttc
     7561 caaaaaaggc ggattgatcc cctgtcggca acttgaccgc gccgcggagg gaaacagctg
     7621 gcaccgaacc actttccgtc aggaactgga acttgctcat cagcgtggta tcccccagcc
     7681 ccgttgcacc aggacctccg ctcatcacgg tttgtccgtt acgagtgacg ttgaaagcat
     7741 aactggtgtt gctgagggca gctcgtgccg gcgcctcgcc ggtcgtcgcc cgttcggtcg
     7801 ccgtgatcgc cccgttcatg aacccctgat accggtagta cactggaagc tctacaccca
     7861 gttctacctt ctcagtgagg ccatagcgaa ggaagaggcc ggatcttaag gtttcaaatt
     7921 taatgaccgc cgtggcttgc ggctgaatct cgttatagat gctggctgtc tccgcaagct
     7981 ccaaccgtag gtccagtgta ccctgtttca gaaccgacgc acggtcacct ggaagattta
     8041 agaccagttg ctggaaagcc tggaaattcc ggactgggaa tggaccgaac ccttcggccc
     8101 atgcgacaga tgaagagagg gcgagcaccg ttagcgcccc tgcaagcaaa cggactattc
     8161 gattgggata accgatcttc ctgctcacaa ttgtctcagt aagggaaatc aagcccggcg
     8221 aacacagcgc tcccctcttt gctaaaacca aggtcgatcc gaccgacgac attcggtcga
     8281 acgatcccgc ggaaccctat gccgggcgtt atccgataat ctttgagact gacgtctttg
     8341 aaattattga agacctgtcc ggtatccaca aacggcgcaa tctcgaaatc ggccgcgacg
     8401 ccggcgactt ttgccctggc cacgtgaatg cgctcttcaa tgctcgccgc gatcagatgc
     8461 ttgtcgatat agcggtcgac cccgtatccg cgtaggttgt tttgcccgcc caatgagctc
     8521 tgctcgaaaa acgggacctg gtcacccaac gtggcttgca ggtcggcgcg caaaaccaga
     8581 atggctcgct tggattcgct ggggaacagc ttcttcactt ccaactcata gcgggaatag
     8641 acgggatgat cgccgttgtg gacattctga ttgacctccg cataggccgt cacagccatg
     8701 ccatcggtcg gcgaggagag attattacgc gtgtcataat agaaggtcgc acgatgcccc
     8761 acgatgatcg tctcgccgct gacgccatcc acagtcggga atacctctcc tgtgaatggg
     8821 aggtccgtcc cacccctctg aagactcacc tgacgaaagc gctggctgac ggccacttgc
     8881 gtcacctcat tcgcatagac accgaatcgc cagtttgccc gtgcctcgcg ggacgtgtag
     8941 ttcgtttcac tcgattcctg agtctcctgc cccaatccaa agaagcgcgc cgtcgcgttc
     9001 ttaaaaaacg agccgccgaa attcagaaag taccgtccgt tactgaacgc cggatccgta
     9061 tagttgaaca cgagcttgcg ctcaatgtgt tccgtgtatg aggcgatgaa ctgcatctgc
     9121 cgcccgccgg tatcgtaccg aaacaagtta aatgtgcctc tggttcccac gatcgaattt
     9181 cggatgatca tcggggccaa aaggtatttc aactctccgt cgggatcggt gatgagaatg
     9241 ggaacaatta atccagcatc gttcccatcg ttcttggagg tcgaggcgga agggatggga
     9301 aaaacctgag tgtcagcgca ggcggactcc acccacgcca tcccgaaccc tacgagcagg
     9361 catacagtga aaacaacgat gccgtgcagg gtcgtcatga gcgctcgatt cgactatatt
     9421 agggcgtctt gatcttatcg gattttttgc ggagttgatc gatcaaatca acgtaggttc
     9481 cggtgcggag aatcttggtg aactgccccc ggtagttgtt aacgagactg acgccatcga
     9541 ccacgacatc gtagacccgc catactgagg ccttgttgag caggcgatag tcgagtggaa
     9601 tttccgtttt cccggtcagc actttcgtcc gcacttcggc atagtctttt tccgtccgct
     9661 cattaaggta ctgcacacct tcgcctgaat aggactcgat tttatcggca tacgaattca
     9721 ccagcagtgt ttggaagagc acgacgaatt cgtctctctc cttcgccgac agattggccc
     9781 agggggcccc gagcgcacgg cgtgacatct cctgataatc gaaacgttca cccacaactt
     9841 tctctagcaa catccggcgc tcctctgatt ttcccggctg cttcaaatcc ttatcctgta
     9901 tgatcctgag cacctcatcg attgttgcct tcatcgcatc cgtcgctcca ccggcccata
     9961 cattccctgc ggtcagtcca ttgcatagga gcaacgatag gacgatgctc atgcagattg
    10021 cccgaccaat acctcgtttt cccgcgacag cacacgtcat gatgcatttc tcctttttcc
    10081 ccgctcccac atcgtgaaga gcccgaccca catatagtta gttcactttt ccatggacgt
    10141 actggctcac caattcttca agatcgagcc cggattccgt atcgcgaatg gtatcaccgg
    10201 gtttcaaagg atcgccacct ccccccggtg aaagcgcaag gaacttttcg ccgatgatcc
    10261 cccgcgtttt gatcgatgca attgtgtcgg tatagagctt gacgctgtcg tctactgcaa
    10321 gcgtcacagc cgcacggtct tccttgagga cgatggcccg aacccgcccg acttcgactc
    10381 ccgcgatttc gatggcagag cccggcttca ggcctgatgc cgaggtaaat tgcgccacca
    10441 cttcgtagac attcccgccg atgagttcca gcttgcccag cttaatcgag agatatccca
    10501 gacagagaat gcccaccagc acgaacacgc cgacaatcaa ttctaatttg gctttttcca
    10561 tccgttcacc cctcttgatt agccaccgga gtccccggca aagtgaccgg cccgctaacg
    10621 gaaataaact cgtgaatctc cggatctgtt gtcttgacga actccgccgc aggcgccatt
    10681 gcggcaattt ttccctttcg caacatcgcg acccagtcgg agatcgcaaa gatctccgga
    10741 atctcgtggc tgaccataac ggcggtgaac ttgaaccgcc gttgcatccc cacgatcaaa
    10801 tcgtggatcg atttggccat taagggatca agcccggtgg taggctcatc aaacagcatg
    10861 atctcgggct ccatcaccaa ggctcgcgct aggccggcgc gtttccgcat gcctccgctc
    10921 aattcggccg ggaacttgtg ccccatgccg gccaacccta cctccgccag tttttcctcg
    10981 acccgccgca tgacctcggg ccccttcatc cgaagtttct cccgcaacgg gaaggcaaca
    11041 ttctcggaga ccgagagtga gtcgaaaagc gcagcgcctt gaaacaacat ggcaaagcgc
    11101 ttccgtacgt cattgagacg ggtcccccgc aatcgggaaa tctccacatc gccgacccac
    11161 acttcgccac ggtccggctg catgagcccg atcatgtgtt tcaacaacac gcttttccct
    11221 tcgccactct tcccgatcac ggttgtcagc ttcccggcag ggatgctcag gtcgacccct
    11281 tgcaacaccc gctggccgcc cagggtcttt tccacgccga cgagtttgat catagcgttc
    11341 agcaccgtga ctgaattcct acttgtaact caccactcat gtctcagcac tcagcactac
    11401 tacaagagca ccgacgttaa gaaatagtcc cacacgagaa tcagcacgga cgagagcact
    11461 acggcttcag tcgtggcggt tcccagccct tcggcacttt gccgggtgaa gaatcccttg
    11521 tagcagcaaa tccaactcac gattagtcca aaactgatcg acttgaggat accgccgtaa
    11581 acgtctttcc attcgaccgc ggactcgatc gaattccaat aggctccacc gttgcccccc
    11641 agaagatcca cgccgaccag ataccctcca tagatcccga cgacgtcgaa aatcgcgacc
    11701 agcaacggga caccgatcag tccggcaacg agtttcggcg cgatcagata ctgcagcggg
    11761 ttgatcgcca tggtatcgag cgcatcgatt tgctccgtga tcctcatgat gccgatttcg
    11821 gccgtcatgg cggatccagc acgtgccgtt accatcaacg cagcgagcac aggacccaat
    11881 tctcggatca tgctcaacgc gacagcagag ccgaggagcc cttcggaacc gaacttcctg
    11941 agcgtgtaat agccttgcag ggcgaggacc atccccgtaa atcctgccgt gaggaccacg
    12001 acaaatgtcg atttgtaccc gataaaatgc agttgcttga cgatttgcgt cagccgaaat
    12061 ggaggcctgg tcagccaggc gaatgacgac agcacgaaca gcagcatgcg tcccatttcc
    12121 tgaatcaatg ctataaccct tgcacctacg cgttcaatca tcgcctacgc cctgtttaca
    12181 aggtttgaga aagcccgaat cctcttctcg caaaggcggt atgcaccgcc gtcagctgat
    12241 ctgcagccag gccgctctgc tgtcgcagcc gattcatacc gagcagcttc gatggagacg
    12301 cgagcaattc ccacattccc ctcagccatc cggcaggctt gagaaacaga ttgaagtcca
    12361 ccgggagttc ttcaccgacc aggtccgaaa cggttcggac gaccatgaag ggaaccgccc
    12421 tttcctgcgc cacgagtgcc agtgcggcac tctccatatc gagagccgtt gctcccgtca
    12481 tgcggctgat cgcctgtttg tcctctgtac gatagacaat ccggggcact gacacgaccg
    12541 gaccgagatg agcagcaatt cctacttgcg ctgcgacgga ctgaacgcag ctcaggcccg
    12601 cgtggtcgca cgagacggcc tctgtgttct gcctccaggt acgatcatac gacccggcac
    12661 acacgctcgt tccaattacc aaatctccga tcgccgcagg cgtcaacgca ccagcaaacc
    12721 ctgtagagat gatcagggcc atgggctgac gattcaggac cgagcgggcc gccgtgctcg
    12781 ccgcgtcgga tccgaccccc gtttgaatca accagtaagg gcgattacct tgacggccga
    12841 tgagacagcg gataccaccg acgtgttgtc gccgatcaac cggcagagcc cgccgaagcg
    12901 cggccaactc ccagcgcgtt gcgacaaaca tcgccacagg attcacctga gactcggcat
    12961 ggaactgacg agcgtcacgc gatgacgtcg gctgctgggt caacgaccag accgatagca
    13021 cccggctgtc agaatctgat gccgcacttc atcggcccgc attttattgc gcgtgcgcag
    13081 attccgatac atcgcgagtg cccacagggg gaagtactgg cagtaccagt gatagcgaag
    13141 gtagaacacg cgcgggaacc cggtgcccgt atgacgaact tcttcccacg aaccatcctt
    13201 catctggtgg cgaaggagaa attgaatgcc gcgtgccacg ctgaacgaat ccgtcatacc
    13261 ggccgacatc aaccccatga gggcccacgc cgtttgcgac ggcgcactct cccctctccc
    13321 ggatgccgcg gcatccgcat acgacagaca ggactcgccc caacctccgt ccgggttctg
    13381 tttcgattcg agccaggtga cggcacggcg gatgtagggc tgcgagagat cttctccgat
    13441 cgcccgcagg cccgcgagca cggaccaggt cccgtagatg taattcaccc cccagcggcc
    13501 ataccaactg ccgtccggct cttgttcgtg tttcagaaac gccagagccg gcgccacagc
    13561 gggatgggtt tgatcgtagc ccagcgcagc gagcatctcc agacaacggc ccgtcaggtc
    13621 ggcagtgctg gggtcaagta acgcatggtg gtctgcgaac ggaatgtaat tgaagacgat
    13681 tcgattattg tccttgtcgt aggcccccca gcccccgtct gatccctgca tagccacaac
    13741 ccaagccgcg ccgcgccgaa tggaatcttg caactcagcc gtccgtccca ttttcacctt
    13801 cgataacgcc atcaacacga cggcggaatc atcgacgtcc ggatagagtt cgttttcatg
    13861 ctggaagtac cagccccctg gttcagcatg aggggaagaa catttccaat cccccaccgc
    13921 tcttgtctga cgggacatga gataggccgc tcctcgttgc agcgccggat ggtcctgcgc
    13981 catccccgat tcgacaagag cattcatgag caacgacgta tcccatatcg gagaatggca
    14041 gggttgcaaa tgcaacgcat cgactcgctg ctcgccgacc gaaatcgtat cgtagacttc
    14101 cagatcttcg atttcctgaa gcgccttctg caccaaggga tcatcgacct gataccccaa
    14161 gcattgcagc gccatgatcg aattcgccat ggccggatat atagcgccga gccccccact
    14221 acccttgatg tgttccaaca tccaggtcgc ggcgcgatgc aacgcttttt cgcgcagcca
    14281 catgagcggc atgcggtcgt agagtttcag catcgcgtcc aatgccacaa agaaattgtg
    14341 gggggtgaac cacttttgat ccttattgaa cggcggaaac ttccaatagc ggatctcgtt
    14401 gcgggggatg agatacagct catcaatccc ctgttccttg ggcagcttgc agaccggccg
    14461 gtgcgcaaac acgattagca gcggaatgag gacggtccgg gaccaatacg aaatggcata
    14521 gatgttaaag tagaatcctt tgggaagcag catcaattcg accggcatgt gtggaatgcc
    14581 ttcccagtcg tattgatcga acagcgccag cgcgatcttg gtaaacacgt tcgactggac
    14641 gacgccaccg tgtgcgcgaa tgcaatcctg tgcgcgcagc atgaacggct cgtcagcaga
    14701 aatcccgctc aacttcaacg caaagtaggc tttcaccgag gcgctgattt ccgaagggcc
    14761 gccgtaatag atcggccacc cgccgtccgg cagctgtgtg gcgcgaaggt agcgcaccgc
    14821 ctttcgttcc cgctcgggat cgacccggtc gaggaatcgc cgaagcatga gatactcgga
    14881 ggtcagcgtc gtatcggcct caagctcggc cacccagtag ccttctgcgg gatgctgtct
    14941 cgcaagaaac caggcctggc ttcgccggac cgcatcgtca atcgcgtcca actggctgcc
    15001 catttggctg gcggatcgcc tggtcgagga gtcggcagtc ggcaaggccg cggacttgtc
    15061 tgaaaccaac cgcaacggcg gctggctgga gacatcggcg gccagtctca ttttttgggg
    15121 gacagagata aacagtcgat cggagaggcg atcgaggatc gttcgaatca gtttcatcgt
    15181 attctacgag cggaaaacca gtacgattgg gcccaggtcg cgcagaacta ccccccctcg
    15241 gcatcgcatc cgcacgaacc gagcgagctc gacctcataa tttgtcggga tataacagac
    15301 agccttaccg aagtcaagta aacggaatac ggaagtcctc tgaagtacga atgtttttgg
    15361 ccaacctgcg aacttaaacc ggttgcaaat cggtcagtcg gaccgacacg agtttcgaga
    15421 tcccgggctc ctccatggtg accccgaaga gcgaatcggc aacccccatg gtccgcttat
    15481 tgtgcgtgat gaccatgaac tgggcaccct gagccagctc acacaataca gccgtaaatc
    15541 gtccgatatt ctcctcatcg aggggggcat cgatttcgtc gaggatgcag aagggcgtcg
    15601 gtcggattag gaaactggcg aacaacaacg ccatcgccgt cagcgttttt tcccctcccg
    15661 aaagcatcgt gatgttcttt aaccgtttcc ccggcggttg cgccacaatc tcgacacccg
    15721 gttcttgagg acctttgccc tccgcatctt catccaccgg cacctcaaca agctgcagtt
    15781 ctgcgcgtcc cccagggaaa aactttgtaa acacctcccc gaatttttcc tgcagctccg
    15841 caaatgtcgc cgcgaacata tccttggtcg ttctgttgat gcgctggata atctccttga
    15901 gggacccgat cgaggttgag agatcctgtt cttgcgtcga cagaaacttc cagcgctctt
    15961 ctaattcttg atgctcattg atcgccgcca gattaatcgg ccccatccga tcgagacgct
    16021 ctcggatctt ctggatctgc tgcctgagat catcttcagt cggcgtcgat gccacttcga
    16081 ccgttgattc cgcctccccc tgcggtgtcg caaaggctgc gcccgcaaca gacgcttcga
    16141 tcaacgacat gggatcaacc tggtaggtgc cggatagcgt ggattccaca gttcccagtt
    16201 gagtccgcac ctcggctcgt cgcacttcaa cgctcatgcg tgactcccgc aaggccgaaa
    16261 cggttttccg cacggcatcc acagcggttt caatctcttg cgcctgcatc atgtcttgcg
    16321 cctgcccttc ctgggcttcg atcaaccgtg acttcacctg ctccgccgac tggcccagtt
    16381 cgcggcacac acattcctgg cgcgttcgtt cctcacgact ctgctcgagc gaagtccgga
    16441 gagactccag ctgccggctc aacgtctcga tgcggctcac ggcttcctgt ttctcctgac
    16501 gaatccgctg aagatcgctt ccatggtgtt cccgcgtcgt gcggaggtct tgcacggcca
    16561 actgggcctc agtgagccgt tgctggagcc cctgtatccc ttgatcgagt tggatcagac
    16621 gcgcccgcac ggcctgtagc gctccttcct ggccggattt ctccgcaatc cattgagcca
    16681 gctgtgcgtg accggagcga gtctcttgct cgagtcgctg cacttcgagc gagccgcgct
    16741 gcgcatcccc catcaacgtc tcgattcgct gcgtcaaatc accgagaagc tgctgcgccc
    16801 caaccacatc cttgtgcagc gaaagctccc gcatttcccc ctcgcgcaac gcatcagcaa
    16861 cccgacggcc ttcttcccgc aagaattgac tttccgccag caactgttcg cgcctttggc
    16921 gctctgcctc gacggactcc atcagcgata tccgccggga ttccaactgc aacacttcgc
    16981 gccgccgctg cagcaatccg ccagtcgaac tgacctgtcc tccggtgaca acgccggccc
    17041 catccaagac ttcccccgca cgggtgacga acgtgggacc atcgtttgca gaccatgtgt
    17101 gttgttccca taattgcatc gcctcttgga gggattcgac gaacaccacg cgatcgaaca
    17161 gataatcgcg cgcagcgtca cgatgacctt ccacctggac aagctcgacg gcccgcccaa
    17221 taaccccggc ctggccggcg atggcctgcc accaatcggc ggaaccggca gcttgcgcag
    17281 tccaacgcgg agattccgga ataaaggttc cccttccaag atctttcccc atgaggaagg
    17341 caatggcacg ggcggcagtc tgcggacttt cgacgaacca accgcgaact ctctcgccca
    17401 aaatggcttc gacggcccga tccaaaccgg ccggcaccac gagccattcc gccacagcct
    17461 cgtgcacgcc ttcgcaggtc ttgagtgcgg ttgactcttc atcgcccgat cgtccgtacc
    17521 ccatgtcctc ccgcaacact ccctgaagcg cgcggagatg ggaatccacc gcagccaact
    17581 cttccgactg actcaacaac cgttggtcga catcggtgag tccggtcgca acctgctccg
    17641 ccttttgtcc caattgttgc tgctgctcgc ggagttcgac gacgacccgc tctgcctcct
    17701 gactggcctg ctcaagcgcg tggcgcttct ccctcgtcga atcatgctgg gattggatct
    17761 cttcgcgttc gcgggccaaa cgcccatcgc gttctgcgac ctcctcagcc cgcaacgcca
    17821 actgcgacag gctctgctcc gtattcgcaa ccaagacggc caggttcagc acgtcgagtc
    17881 gcccccgctc ttcttcctga atggccccag cccgttggtg caagagcgac ttcatctctt
    17941 gatcgagtcc ggcgagcacc tgctcacgct ccgtcaattc tcgctccagt gtctcgagcg
    18001 tcacgagcag agccgtttcc gccgccttca cttcttcctg ttcctgcgtc agacgctcga
    18061 gttcttgagc gccctggacg cgctgctcct cgtacatctc actccgatga cgctcaactt
    18121 cggcggcggt taaggcctgc gcctgttgct gctcgatctt cgagaattcg tcacgccgtt
    18181 gatcaatgac gctgccggcc tcggcaatgg ccagcttgat ccgctcgagt tcggcgttga
    18241 accgggcctg ttcggccacc cgctcggact cttcatgaaa caaaccctcc agctcccgct
    18301 cggtatccgc gatggtggag cgcaatctcc gatactcgtt ggtcagcaat cgaatctcga
    18361 gtgcgcgcgc ctcctgctga agcacttgat aggaccgcgc ctgacgcgcc tgacgctcaa
    18421 gggaattcag ctgctttttc acctcggcga tgatgtcgcg gacgcgcaga agattctgtt
    18481 gagtggcatc gagcttccgc agggcctccg ccttttgctt tttgtaccgg atgatgccgg
    18541 cggtttcttc gatcaactcc cggcgatctt gcggcgacgc gttgagaatc tggtcgatct
    18601 ggccctgggc aatcaccgtg tgtcccttgg tgcccgctct ggtatccagc aacacgctgc
    18661 gaacgtcttt caaccggcag agggtcttat tgatgaggta ctcgctatcg ccgttcctgt
    18721 agaggcggcg cgtgatcatg agatcctgat actccgacaa ctggctggga agaccggact
    18781 gactgtcgac
//
LOCUS       DATIFR010000248         9856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_368817, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000248 DATIFR010000000
VERSION     DATIFR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368817"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VLD60_14145"
     CDS             complement(<1..467)
                     /locus_tag="VLD60_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB46151.1"
                     /translation="MGSVRQGHSVGKPASIAAARRGGTVSATALRPYIERFPRASILV
                     IGDLILDHYIWGRVSRISPEAPVPVVHVESESVRLGGAANVFNNILALGGKADLCGLI
                     GSDEAGRILLKELGSKRSGRGGVVIDQERPTIKKSRVIAHNQQIVRYDIERKG"
     gene            complement(437..1189)
                     /gene="lepB"
                     /locus_tag="VLD60_14150"
     CDS             complement(437..1189)
                     /gene="lepB"
                     /locus_tag="VLD60_14150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSB46152.1"
                     /translation="MSVDQHPHGSEDVATPSTDAAAGGVSAMAGPTRKSIVREYAEAI
                     VVAMLLAFAIRVFVVQAFKIPSGSMIPTLLIGDHILVSKLSYGIQWPNDCKFQMAFPP
                     VNCYASQAIIEFGKPQRGDIIVFRFPEDEEKDFIKRIVGTPGDTVQIKNKMVLVNGMP
                     LDDKAFTQRIDPGIIDGTINPRDNFGPVTVPEGSYFVMGDNRDQSLDSRFWGYVREEK
                     IRGKAFRIYWSWSGQGQWKEWVRWDRFGKAIQ"
     gene            complement(1186..2985)
                     /gene="lepA"
                     /locus_tag="VLD60_14155"
     CDS             complement(1186..2985)
                     /gene="lepA"
                     /locus_tag="VLD60_14155"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSB46153.1"
                     /translation="MQSLIRNFSIIAHIDHGKSTLADRFLEATGAVTAREAKEQILDA
                     MDLERERGITIKAHAVAIRYKAKDGKTYALHLIDTPGHVDFTYEVSRSLAACEGSLLL
                     VDATQGVQAQTIANVNLAMANKHVIIPVINKIDLASADVENTKHSISEVLMLDASDAM
                     PISAKEGRGVPEVLEAIIERIPPPSGDPNATLKALIFDSWFDNYQGVIVLTRIVDGSV
                     RPGMKIKVMSNGRAFEVMEVGQFTPKRVKKTELLTGEVGYLCANMKEVADVKIGDTIT
                     DAVLPTSEPFPGYKEVKPLVFCGLYSTDTAKYEDLRDALVKLRLNDSSFVYEPETSLA
                     LGFGFRCGFLGLLHMEIIQERLEREYGLTLITTAPTVVYRVTSTKGEVLEIDNPAKLP
                     PPNSIQLFEEPFILATVITPERYMGAILQLCQERRGIQRSIHFLDPTRVMISYEMPLN
                     EVILDFYDKLKSRTQGYASLDYELLGYRESDLVKLDILLNGEAVDALSFITHRDRSYP
                     RGRQLAEKMKELIPRQMFEIAIQAAIGNKIIARETIGAMKKNVTAKCYGGDITRKRKL
                     LEKQKEGKKRMKAVGSVEVPQEAFLAILKVGEE"
     gene            3142..4521
                     /gene="bioA"
                     /locus_tag="VLD60_14160"
     CDS             3142..4521
                     /gene="bioA"
                     /locus_tag="VLD60_14160"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HSB46154.1"
                     /translation="MIRRKHTASLSNWDRRYLWHPFTQMQEWEQEDPLIIERGMGPYL
                     IDTNGSRYLDGTSSIWVNLHGHRHPSLDRAIKKQLEMIAHSTLLGLSNPPAIRLAREL
                     IRIAPNGLRRVFYSDNGSTAVEVALKMAVQYWKQAHPDAGPKSSFLHMKLAYHGDTIG
                     AVSVGNIELFHSRFKPLLFPTLEAEPPYCYRCPLKLAYPSCNLTCLDPIEQILKTRHR
                     ELAGFIIEPLIQAAAGMITQPHGYLKRIRNLCTKYQVLLIADEVATGFGRTGKMFACQ
                     HEGVTPDLMAISKGLTGGYMPLAATLTTEKIYKAFLGKYEDFKTFFHGHSYTGNQLGC
                     AVALANLEVFRKEKTLTRLRPKIRMLSRLLRPLRTLPHVGEIRQQGFMAGIELVQDRT
                     TRVPYPLTSRIGHRVALEARRQGLILRPLGHIMVLMPPLSTDLKILSRMVEIVGRSIK
                     TVTMNDHSS"
     gene            4659..5894
                     /locus_tag="VLD60_14165"
     CDS             4659..5894
                     /locus_tag="VLD60_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46155.1"
                     /translation="MRRSLLAVGLLLSTLSLGPQAWGGDDHSTDQGIQRWASLLSEQH
                     AQADSFSKQTAVPFFQASVTSTFALPPIMSPRLRDLSPETDCPRTRGLLATTTWFKGT
                     FVTETEVANSLGGASWLQNTIPGDTRDDASRRMIRLGLTGTQGTLRYGLMYRSAGQGF
                     LNAPDQAGREIWSEWKASWATLRSAVGQLWNNVAGETTRARLTQTYGRMGLILTRPSW
                     PELSMTYARNSFSSSLEPLGIAPQQTQSHTLEGALAYQSLRWNVRVASSYALISDLLR
                     GGAESNVRMQILSASFHPLNTITIAPMLAYREETQDWSGTRIDGPSASLALHYKQSRR
                     LLVSAMGNYASSRSSDRLIENENLGGKGILSWDIQQSSRWNARIAIEARYNHLTNRVI
                     PSADTEDISGLVRIVVAAL"
     gene            complement(5964..6788)
                     /locus_tag="VLD60_14170"
     CDS             complement(5964..6788)
                     /locus_tag="VLD60_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSB46156.1"
                     /translation="MDFQKPSSVPAARALITLLTDFGDRDWFVASMKGVILSINPLVT
                     IVDLSHHITSHAVQDGAYVLKSCYRYFPDGTVHLAVVDPGVGSTRRPLIVKSSRYYFV
                     APDNGLLTHIFAQEAEVEVREIENKQFRLESQGHTFDGRDLFAPAAAWLTKNQPFPSY
                     GSVLREYETFQISVPHWESMTLVGEIVHVDQFGNLITNLTRDHLKEVQAVSKRPNAAV
                     RIAGQVIDGLVGSYSEGVISSPRALINSDGQLEIFCKESSASAHLKVSRREVVKLS"
     gene            complement(7038..7514)
                     /gene="greA"
                     /locus_tag="VLD60_14175"
     CDS             complement(7038..7514)
                     /gene="greA"
                     /locus_tag="VLD60_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSB46157.1"
                     /translation="MPTPITKKGYDALKAELDRLRKVERPQVIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIESRIAEIETKLADARVVEIAGRVSDTVVFGATVLVIEQESQSKRQ
                     YTLVGQDEADMKFNRISIQSPVGRALIGKRVGDFVEVKTPVKTVEYELVEIRFEEL"
     gene            complement(7529..>9856)
                     /gene="carB"
                     /locus_tag="VLD60_14180"
     CDS             complement(7529..>9856)
                     /gene="carB"
                     /locus_tag="VLD60_14180"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSB46158.1"
                     /translation="SKATGFPIAKIAAKLAVGYTLDEITNDITGVTKASFEPTIDYVV
                     VKIPRFAFQKFKGADPTLTTQMKSVGEVMAIGRTFKESLQKAIRSLELDLSGLASRLG
                     LDRGIPEGFDRLNSVERISQQLRTPIPERIWYVADGIRLGMSNEELFALTKIDPWFLE
                     QMRDLIRFEGILVGQSANSPALLEEAILWEAKELGFSDERIARLTGIAVTSIRKQRTT
                     SARGTRAVTYKRVDTCAAEFEAHTPYLYSTYGSECEARPTDRKKVIILGGGPNRIGQG
                     IEFDYCCVHAAMALREEGIETIMVNCNPETVSTDYDTSDRLYFEPLTEEDVLNIVQCE
                     KPIGVVLQFGGQTPLKLAIPLSKAGVRILGTSPDAIDRAEDRERFRDLLNKLGLRQAD
                     SGIARSVDEAVAIASRISYPVMVRPSYVLGGRSMQIVYDEDGLLNYMGSAVKASPAHP
                     VLIDKYLSDAIEVDADAISDGRQVVVAGIMEHIEEAGVHSGDSGCSLPPYSLDKTVVE
                     EIRRQMVALALELEVVGLMNAQFAVKDNTVYVLEVNPRGSRTVPFVSKAIGVPLAKLA
                     MKTMVGKSLSALGFVSAPTPSHLSVKESVFPFNKFPGVDVLLGPEMKSTGEVMGIDAD
                     FGWAFAKSQAGAGAILPSGGTAFISVKESDRPAALEVAKRLHRLGFSIQATSGAAAYL
                     RSCGLDVKTVHKVTEGRPHIVDHIKNGEIALVVNTVGTAASHADSLSIRRETLNKGLA
                     YYTTMRGARAAVMGIEAMLKKELTIRSLQEYHHVS"
BASE COUNT         2134 a         3001 c         2560 g         2161 t
ORIGIN      
        1 tcccccttcc gctcgatgtc gtaccgaaca atctgctggt tatgcgcgat gactctcgac
       61 ttcttgatcg tcggccgctc ctgatcgatg acgacaccgc cacggccgga ccgcttcgaa
      121 cccaactctt tcaggagaat ccgccccgct tcgtccgaac cgatcaggcc gcagagatcc
      181 gctttgccgc ccaacgccaa aatattgttg aagacgttag cagcgccgcc gagtcgcacg
      241 gattcggatt ccacgtgaac gaccggtacg ggcgcctcgg gagaaatccg actgacacgg
      301 ccccagatat aatggtcgag gatcaggtcg ccgatcacga gaatggaggc tcgcggaaac
      361 cgttcgatgt acggacgaag ggccgtggct gatacggttc cccctcggcg agcagccgcg
      421 atggatgctg gcttgcctac tgaatggcct tgccgaaccg atcccatcga acccactcct
      481 tccattgtcc ctgaccactc catgaccagt agatgcggaa agctttgccg cggatcttct
      541 cctcgcgtac atacccccaa aaccggctgt ccaggctttg atcccgattg tcgcccatta
      601 caaaatagga cccctccggc accgttacgg gcccgaagtt gtcgcgcgga ttgattgtcc
      661 cgtcgataat gcccggatcg atccgctgcg taaaggcctt atcatcgagt ggcatcccgt
      721 tcaccaggac catcttgttt ttaatttgaa cagtgtcgcc tggcgtaccg acgatgcgtt
      781 taataaagtc cttttcttcg tcttccggaa aacggaacac gatgatatct ccccgctgcg
      841 gttttccaaa ttcgatgatt gcctgcgagg catagcaatt caccggtgga aacgccatct
      901 ggaacttaca gtcgttgggc cactgaatac cgtacgacaa cttgctgacc agaatgtgat
      961 cgccgatcaa cagggtggga atcatcgaac cggaaggaat cttgaacgcc tgaacgacga
     1021 acacccgaat ggcaaaggcc aacaacatcg ccacgacgat cgcttcagcg tactcgcgaa
     1081 caatcgactt tctggtcggc cctgccatcg ccgagactcc accggccgcc gcatccgtgg
     1141 aaggcgtcgc cacatcctca gagccgtggg gatgctgatc tacgctcatt cttcaccgac
     1201 tttcaaaatg gccaggaacg cctcttgcgg cacttcgacg ctgccaaccg ccttcatgcg
     1261 tttctttcct tctttttgtt tctccaacag cttgcgcttt ctcgtgatgt cgccgccgta
     1321 acacttcgcc gtcacgttct ttttcatcgc cccgatcgtt tcgcgggcga tgatcttgtt
     1381 cccgatcgcc gcctgaatcg caatttcgaa catctgccgg ggaatcagct ctttcatctt
     1441 ctcggccagc tggcgtccgc gaggatacga acgatcgcga tgcgtgatga aggacaaggc
     1501 gtccaccgcc tcgccattca gcagaatatc gagcttgacc aaatcggatt cccgatagcc
     1561 gagcagctcg taatcgagcg aggcatagcc ctgggtgcgg gatttcaatt tatcgtagaa
     1621 gtcgagaatc acttcattca acggcatctc gtaactgatc atgacccggg tcggatcgag
     1681 gaagtgaatg ctgcgttgaa tgccgcggcg ttcctggcag agttgaagaa tggcacccat
     1741 gtaccgttcc ggcgtgatca cggtggcgag aatgaatggt tcttcgaaca gttggatgct
     1801 gttgggcggc ggcagcttcg ccggattgtc aatctccagg acctcgccct tggtgctcgt
     1861 tacacgatag acgaccgtcg gggctgtcgt gatgagggtc aatccatatt cccgctcaag
     1921 gcgctcctga ataatttcca tatggagcag gcccaaaaac ccgcaacgga atccaaagcc
     1981 gagcgccagc gatgtttccg gctcatagac gaaggaggag tcgttcagcc gcagcttgac
     2041 caacgcatca cgcagatctt cgtattttgc cgtatcggtc gaatagagcc cgcagaacac
     2101 cagcggcttt acctccttat accctgggaa cggctcgctc gtcggcagaa cggcgtcggt
     2161 gatggtatca ccgattttca cgtccgccac ttctttcata ttggcacaga gatagccgac
     2221 ctcgcccgtc agcagttcgg ttttcttcac ccgcttcgga gtaaactggc cgacctccat
     2281 gacttcgaac gcgcgaccgt tagacatgac cttgattttc atccccggtc tcacgctccc
     2341 atccacgatc cttgtcagca cgatcacgcc ttggtagttg tcgaaccagg aatcgaaaat
     2401 caatgccttg agagtggcgt tcggatcacc ggacggtgga ggaatccgtt cgatgatcgc
     2461 ctcaagcact tccggcaccc cccgcccttc cttcgcactg atcggcattg cgtcgctagc
     2521 atccagcatc aacacttccg agattgagtg cttggtattc tccacgtcgg cactggccag
     2581 atcgattttg ttgatcacag gaatgatgac gtgcttgttc gccatcgcca agttgacatt
     2641 ggcgatcgtc tgcgcctgaa ctccttgcgt cgcatccacg agcaacagcg acccctcgca
     2701 ggcggctaaa ctgcgagaga cctcgtaggt gaagtcgaca tgccccggcg tatcgatcag
     2761 atgcaaggca taggtctttc catcctttgc cttgtaccga atcgccacgg cgtgagcctt
     2821 gatcgtaatc ccccgctcac gctccagatc catcgcgtcg aggatctgtt ctttggcctc
     2881 tcgggcagtg acagcgcctg tggcttcgag gaaccggtca gcgagggttg atttgccgtg
     2941 atcgatatgg gcaataattg agaaattgcg tatgaggctt tgcaaatcct agctcatttc
     3001 ttcaataaaa tcggttcatt atagtaactg ctccccgggt tgtcaaagca atcagcccct
     3061 cgtataatcc gcgccgcacc tgcgccaatc gcggattttc aagcgcaacc tcctctacgg
     3121 ttcccccgcg atagtgatca catgattcgc agaaagcata ccgcttcact cagtaactgg
     3181 gatcgccgat acctctggca cccctttacc caaatgcagg agtgggaaca ggaggacccg
     3241 ctcatcattg agcgagggat gggtccctac ctgatcgata ccaacggcag ccgttacctg
     3301 gacggcacct cttccatctg ggtgaatctc cacgggcacc ggcatccatc cctggaccgc
     3361 gctatcaaaa aacagctgga gatgatcgcg cactcgacat tactcggact ttctaatcct
     3421 cctgccattc gattggcacg cgaactcatt cgcatcgctc cgaacggact caggcgggtg
     3481 ttttattcgg acaacggatc cacagcggtt gaagtagccc tgaaaatggc ggtgcaatat
     3541 tggaagcagg cgcatcctga tgccgggccc aagagcagtt ttctccacat gaagcttgcc
     3601 taccatggcg acacaattgg cgccgtcagc gtcgggaaca tcgagctgtt tcactcacga
     3661 ttcaagccgt tgctctttcc cactctcgaa gccgaacctc cgtactgcta ccgctgtccg
     3721 ctgaagctgg cctatccctc ctgcaatctg acctgcctgg atccgattga gcagatcctc
     3781 aagaccaggc atcgcgaact ggcaggcttt attattgaac cgctgatcca agcggcggcg
     3841 ggaatgatta cgcagccgca tggctatctg aagagaatca gaaatctctg cacgaaatat
     3901 caggtgctcc tgattgccga cgaagtggcc actggtttcg gccgcaccgg gaagatgttt
     3961 gcctgccagc acgaaggcgt tactccggat ctcatggcca tcagcaaagg cctcaccggc
     4021 gggtacatgc cgctggctgc aacgttgacg accgaaaaga tctacaaggc ctttctggga
     4081 aagtatgaag acttcaaaac attcttccac gggcatagtt atacaggcaa ccaactcggc
     4141 tgcgccgtgg cgctggcaaa cctcgaggtg tttcggaaag agaagactct cacccgacta
     4201 cgacccaaga tcaggatgct ctcacggctg cttcgaccac ttcgtaccct cccgcacgtc
     4261 ggggagatcc gtcagcaggg cttcatggct ggtatcgagc tggtccagga tcgtacgaca
     4321 cgggttcctt atcccctcac gtctcgaatc ggccatcgag tggctctgga agccagaagg
     4381 caagggctca ttcttcgacc actcgggcat atcatggttc tgatgcctcc cctctcgact
     4441 gacctcaaga tactttcccg catggtcgag attgtcggcc ggtcgatcaa gaccgtcacg
     4501 atgaatgatc actcttcctg agcatacgac ctctccctgg tcaccacccc gcaatccgat
     4561 tcggcggata tccggattag aacgcgtttt caatatatta ggggacaaat ttatgagtgc
     4621 tagacttcag agggacaggg gagttcaggg ggttctgaat gaggcgatcc cttctggcag
     4681 tcggtctgtt gctctcgacc ctttccctcg ggcctcaggc ctggggtggc gacgaccact
     4741 ccaccgacca aggcattcaa cgctgggcct cactattatc ggaacagcat gctcaggcgg
     4801 actccttcag taaacagacg gctgtgccgt ttttccaggc ctccgtgact tcgacgtttg
     4861 cgctgcctcc aatcatgtcc cctcgcctgc gcgatctctc accggaaaca gattgtcccc
     4921 ggacaagggg actcctcgcc acgacaacct ggttcaaggg aaccttcgtc acggaaacag
     4981 aagtggccaa cagcctggga ggagccagct ggcttcagaa cacgatcccc ggcgataccc
     5041 gtgacgatgc ctcgagacgt atgatccggc ttggactcac gggcactcaa ggaacccttc
     5101 gctacggcct gatgtatcga tctgccggcc aagggtttct gaatgctccg gatcaagcag
     5161 gtcgggagat ctggagcgaa tggaaagcaa gctgggctac gcttcgaagc gccgtcggtc
     5221 aactctggaa taacgtcgct ggcgaaacca ctcgcgcaag gttgacgcaa acatacggac
     5281 ggatgggcct catcctaacc agaccctctt ggcccgaact gagcatgacc tatgcccgca
     5341 actcgttcag cagctccctt gagccgctcg gaatcgctcc tcagcaaact cagagccaca
     5401 cgcttgaagg cgcactggcc tatcaaagcc tgcgatggaa tgtccgagtg gcgtcaagtt
     5461 acgccctcat cagtgacctg ctccgtggcg gagccgagag taatgttcgg atgcagattc
     5521 tcagcgcctc gttccacccg ctcaacacca tcaccattgc gccgatgctg gcatatcgag
     5581 aagaaacaca ggactggtcc ggcacgcgga tcgatggccc ttccgcctct ctcgcattgc
     5641 attacaaaca gagccgtcgg ctcttggtca gcgcaatggg taactatgcc agttcacgct
     5701 cgagcgacag actgatcgaa aacgagaacc tgggcgggaa aggtatcctc tcctgggaca
     5761 tccagcaatc gtcgaggtgg aacgcgcgga tcgcaatcga agccagatac aaccatctga
     5821 ccaaccgggt cataccctcg gccgacacag aagatatctc aggcctcgtg cgtatcgtcg
     5881 tcgccgccct ctaggagggg gacgaacaca tcgctctcct cactcgccct actccggatt
     5941 cgacaggttc caagaaaccc gccttacgac agtttcacga cctcgcgacg gctcaccttg
     6001 aggtgggcac tggcactgga ttctttgcag aagatttcca gctgaccatc actattgatg
     6061 agagcgcgag gggaactgat cactccctcg ctgtagctcc ctaccagccc gtcaataacc
     6121 tgtccggcga ttcgaacggc ggcattcggg cgtttactga ctgcttgcac ttccttgaga
     6181 tggtcccgcg tcaggttggt gataagattt ccaaactggt cgacatggac aatctctccg
     6241 accagcgtca tggattccca atggggtacg gagatctgaa atgtctcgta ttcacgaaga
     6301 accgagccat aggacgggaa tggttgattc ttggtgagcc atgcagctgc cggcgcgaag
     6361 agatcgcgcc catcgaacgt atgaccctgg gattcgagtc gaaactgctt attttcaatt
     6421 tcgcggactt cgacctccgc ttcctgggca aatatgtgtg taagcaatcc attgtccggc
     6481 gccacgaaat aatagcgcga actcttgacg ataagcggac gccgcgtgct accgacgccc
     6541 ggatctacta cagcaagatg gaccgttccg tcgggaaagt accggtagca ggacttcagg
     6601 acataggcgc cgtcttgaac ggcatgagaa gtgatgtgat ggctcaggtc gacaatcgtc
     6661 accaaaggat tgatcgacag aatgacgccc ttcatgctcg ccacgaacca atctcgatcg
     6721 ccgaagtcgg tcaacagcgt gatgagggca cgcgccgccg gcacgctgga aggcttttga
     6781 aaatccattt tgagaccccg tctcaagggg agcttcgatc accctaacga tctccaccaa
     6841 ttctccagga tgttcaaaaa ggcctctggc atggccacag cgagcgagga agcgaagagg
     6901 tacataccaa gcttcgcttg aaccgctcgc ttcgatcaca ggcgagcgga tagttatttt
     6961 gcctcaagtt ggcttctccg tacgttaagc ctcagatcga tgcgagaacg aagcgggagg
     7021 gctttttcaa cagccagcta cagctcctcg aacctgatct ccacgagttc gtattcgacc
     7081 gttttcaccg gcgtcttgac ctccacgaaa tccccgacgc gcttcccgat gagagcacga
     7141 cccacgggag attgaatcga gatgcggttg aatttcatgt cggcctcgtc ctgcccgacg
     7201 agggtatact gccgtttcga ttgtgattcc tgctcgatga ccaacaccgt cgcaccgaac
     7261 accaccgtat ccgacacgcg ccctgcaatc tcaaccacgc gggcgtcggc caattttgtt
     7321 tcaatttccg caatgcgcga ctcgataaag ccctgccgtt ctttcgccgc gtcatactcg
     7381 gcgttctcgc ttaagtcgcc atgggctctg gcttcggcga tcgcctcgat cacttgcgga
     7441 cgctccacct tccgaaggcg gtccagttcg gctttgagcg cgtcataccc tttcttcgta
     7501 atcggtgtcg gcatgattga tcgttcctct acgacacgtg atgatattcc tgcaacgagc
     7561 ggatcgtcag ttcttttttc aacatggctt cgatccccat cacggcagcc ctggctcccc
     7621 gcatcgtcgt gtaatacgcc agtcctttat tcagcgtctc acggcgaatc gacagtgagt
     7681 cagcatggga tgctgccgtc cctaccgtgt tcacgaccaa ggcgatctcg ccgttcttga
     7741 tatggtccac aatgtgggga cgaccctccg tgactttgtg gacggtcttg acgtccaagc
     7801 cgcagctcct cagataggca gcagcgccgc tggtcgcctg gattgaaaat ccgaggcggt
     7861 ggagccgctt ggcgacttcg agagccgccg gacggtcgct ttccttcaca ctgatgaagg
     7921 ccgttccgcc acttgggagt atagctcccg cgccagcctg cgatttggcg aaggcccagc
     7981 caaaatccgc atcgatcccc atcacttctc ccgtcgattt catctccggc ccgagcaaca
     8041 catccacccc tggaaacttg ttgaagggaa acaccgactc cttgaccgat agatgagaag
     8101 gtgtcggcgc actgacgaat cccaacgccg agagactctt gccaaccatg gttttcatgg
     8161 cgagtttcgc cagtggcact ccgatcgcct tgctgacaaa cgggacggtc cgcgagccac
     8221 gcgggttgac ctccaataca tagaccgtat tgtccttcac cgcaaactgc gcgttcataa
     8281 ggccgacaac ctcgagctcc aaggctagcg cgaccatttg cctgcgtatt tcttcgacga
     8341 ccgtcttatc gagcgaatag ggcggcaggg agcagccgga gtctcccgag tggaccccgg
     8401 cctcctcaat atgctccata atgcctgcca caaccacctg ccgaccatcg gaaatggcat
     8461 cggcatcgac ttcgatagcg tcggacagat acttatcgat gagcacggga tgcgccggtg
     8521 aggccttcac cgcagatccc atgtagttaa gcagcccgtc ttcgtcatag acaatctgca
     8581 tggagcgacc gccgagcaca taggaaggcc gaaccatgac cggataactg atcctggagg
     8641 caatcgccac tgcttcgtcc accgaacgcg cgatgccact atccgcctga cgcagcccga
     8701 gcttgttcag taaatcgcga aaccgttccc ggtcctcggc ccggtcgatc gcatcggggc
     8761 tcgtgccgag aatgcgcaca ccggctttcg acaacggaat tgcaagtttg agcggcgtct
     8821 gcccgccaaa ttgaaggacg acgccgatcg gcttctcaca ttgcacgatg ttcagcacgt
     8881 cctcttccgt cagcggctca aagtagaggc ggtcggaggt atcgtagtcc gtgcttaccg
     8941 tttcgggatt gcaattcacc atgatcgttt cgatgccttc ttcgcgaagg gccatcgccg
     9001 cgtgcacaca gcaatagtcg aattcgatgc cctgcccgat acgattgggc ccgccgccga
     9061 ggatgatcac cttcttccgg tcggtcggac gagcctcgca ctcgctgcca taggtggagt
     9121 agaggtatgg tgtatgggcc tcgaactccg cggcacaagt atcaactcgc ttgtaggtca
     9181 ccgcacgtgt tccccttgca gacgtggttc gctgcttccg aatcgacgta acggctatgc
     9241 ctgtgagtcg cgcgatccgt tcatcggaaa agcccagctc cttcgcttcc cacagaatcg
     9301 cctcctcgag caacgccggc gagtttgctg attgaccgac cagaattcct tcgaaacgaa
     9361 tcagatcccg catttgctcc agaaaccagg gatcaatttt cgtcagagcg aacagctctt
     9421 cattggacat gcccagcctg atcccgtcgg ccacatacca gatgcgctcc ggaatcggcg
     9481 tacgcagctg ctggctgatc cgttccaccg agttcagccg atcaaaccct tccggaatcc
     9541 ccctgtccaa cccgaggcgt gatgcgagcc cgctcaaatc gagttcgagc gaacgaatcg
     9601 ccttttgtaa tgactctttg aaggtccggc cgatcgccat cacttcgccg accgatttca
     9661 tttgcgtggt cagcgtggga tcggcacctt tgaatttctg aaaggcaaag cgaggaattt
     9721 tcaccacgac atagtcaatt gtcggctcaa atgaggcctt ggtgactccg gtaatatcgt
     9781 tggtgatctc gtccagtgta tacccgacgg ccaatttggc cgcgatcttc gcgatgggaa
     9841 atcccgtcgc cttcga
//
LOCUS       DATIFR010000249        23174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_174445, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000249 DATIFR010000000
VERSION     DATIFR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174445"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1614)
                     /locus_tag="VLD60_14185"
     CDS             complement(214..1614)
                     /locus_tag="VLD60_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSB46159.1"
                     /translation="MSESLIHKQADLQGLSTAILLPSVDLFGASARKAVGPLTQVVGI
                     GLFPRTVLTLQRAGIRQLIVLAGPEEDQLKQAMGKGPRVTIPVRWMPIREFPLGDPRT
                     WEALAAEVRGFCLLSGVGGVFSRPLIEQLRQEVQEGQAIVVARESGTGNPSRSKVATR
                     LPLFDSASFETGSSVAADLVVLPASMLSAAGRVPGEPGSVPVRRWLERATADGRVQIV
                     TVGMDSSKWYQAVRDAADVRAAERQLFASLKGELEGFVDRFFNRIVSRWFTRVFLALG
                     FSPNAITMVATVIGLVAAAGFGLGTYSAGIVAALLFQLAAVIDCCDGEVARLTFTESP
                     FGAWLDIAMDNVVHMAIFGGIAVGAFIQAAGSESAWVPLALGAAAVLGNALSFTLVNK
                     AQKIKRASEWKTPVHAAWSDFMLKNVASRDFSVIVLVFAFAGKLDWFLWMAAAGSIVF
                     AMIMVWVLRPSAISRV"
     gene            complement(1684..2403)
                     /locus_tag="VLD60_14190"
     CDS             complement(1684..2403)
                     /locus_tag="VLD60_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HSB46160.1"
                     /translation="MKAIILAAGVGKRLWPVTQHHPKCLIEIGGKTLLHRYLEALASL
                     GIQRADIVVGYKQEMIRAAVQANACGVRVNFLVNEQFHRGSISSLWIARTALDDDVLV
                     MDADVLFHREILRRLVQSPYPNALLMDDTVKQTGEECMVVVEGGRVIALTKKMPPRYD
                     YAGEGVGFLKVRQADTPHVVSSLRTFVDREAWQMEYEDALVEFFREVKVGHEVIGGLP
                     WTEIDFPEDVEKAEREILPKL"
     gene            complement(2400..3482)
                     /locus_tag="VLD60_14195"
     CDS             complement(2400..3482)
                     /locus_tag="VLD60_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB46161.1"
                     /translation="MMLLNPGPVNVTERVRQALLRPDICHRESEFTELLQRIKEKLLK
                     AFVPGAEADYAAVIITGSGTAAVESALMSSIPHGRRILIINNGVYGERLSQMVGLHRL
                     GVSELKYDWTVKPDPERLRLALRQHPEVHGVAMVHHETTTGLLNPVKDIAEVVDSQNR
                     VFIVDAVSALAGETIDIAGSHMYMVAGTAGKCIQGFPGVSFVLVRKGFLERMRNYPKR
                     SWYLHLTHYVNDQGEGTIPFTPAVQVYNAFDEALSELLEEGVAKRILRYKKMATLIRE
                     RMAKMGIKPVLTPERQSNSITAYYLPEGLTYQLLHDRLKGQGYVIYAGQGNLENKIFR
                     VANMGALTEAQFNSFLDAFEQSCKPA"
     gene            complement(3488..4066)
                     /locus_tag="VLD60_14200"
     CDS             complement(3488..4066)
                     /locus_tag="VLD60_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSB46162.1"
                     /translation="MRPEQGTLISRAQAIAALLELLTDQPVVICNGFPSREAHKIADR
                     PTHFYMIGSMGNAPAIALGVALTKPNKQVVVFDGDGNVLMGMGTLATVGALKPKNFIH
                     VVFDNEVYGTTGNQPTISNVVPLEKVAKAAGYVHVERVLDRDDLVYEFKDMLKKDGPS
                     MLLIKVNEFAEDAGRVLHDPPDITKRFMKAIE"
     gene            complement(4063..4560)
                     /locus_tag="VLD60_14205"
     CDS             complement(4063..4560)
                     /locus_tag="VLD60_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSB46163.1"
                     /translation="MIDSDVFVQAMQDMGVNFFTGVPDSILGGIIAELMDRRLYTPAV
                     REDEAVAMAAGAYMAGKVPAVLMQNSGLGTSLNTLISLNMIYRQPCILIVSWRGQGGK
                     DAPEHLVMGEVMPQFLDTMKIPHRTLTEKTAVDDFKWVAETFMKQRIPVALFITKGVV
                     KGLHP"
     gene            4712..5221
                     /locus_tag="VLD60_14210"
     CDS             4712..5221
                     /locus_tag="VLD60_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46164.1"
                     /translation="MRWFLAWLGLFLFLLQPSVSRAVPMSNDPGGFHSIPWGVSLKSR
                     QDVEITREGPHIIEYQLKDAAPVFADIQVDSVRLATVDEQFARVTIRYHGDGTHKQIM
                     TYLERQFGQIERLPGQMMRGLNQQYNWRGSDSEINLTYQANTERGFIFLDSRTLAPRF
                     NDHIADSAE"
     gene            5226..5765
                     /locus_tag="VLD60_14215"
     CDS             5226..5765
                     /locus_tag="VLD60_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46165.1"
                     /translation="MTYQATSGPFQLWSFLAGSILAVLLMCNAVLAVPMIEDPNGFEG
                     IPWGATLSESETFGKVEDAGRFKTYELKASPPTLGPTKVESMKFITIDDRFARVTVRY
                     QGKEAHEKILAWLQTRYGPLDRTPGQFSVGPVKFYAWQGFETEVTMRYEAQMDRGIIF
                     FESQTLRNKVSDGNSPTVF"
     gene            5834..7513
                     /locus_tag="VLD60_14220"
     CDS             5834..7513
                     /locus_tag="VLD60_14220"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HSB46166.1"
                     /translation="MHQATKLRALLKKPGAIKIVGAHDALSAKLIERAGFDGVWASGF
                     AISASLKCIPDASFIDSSEQLGVERRMAEAIDIPIIADCDTGYGNALNVMRTVNDRER
                     AGVAAICIEDNVYPKRCSFYAGVRRELIPIDEHCGKIKAAKAAQTVPDFMIIARTEAL
                     IAGWGKEEALRRAEAYAEAGADAVLIHSKSKTFDELKSVYKAFSGRVPIVVVPTIFDQ
                     TTAAEMEDAGVKIIIYANQPVRAAIRGMRETLELIKTDTRPGAANDRIVTLPEVYDIV
                     GVPQMERDEQAFLPVGSEKITAVIAAAGFEKQLLPLIEDKPKCLLDIKGKTILERQVA
                     ALNECNIKEIALVRGYKKEAITLPNIRYYDNDRYEETGELFSIFCAETEMKGRTILLY
                     GDIIFDNTILEKLLKSPADISLVVDLAWHDQQHLGAQPAHLNPDLVSLAEPPGKSYHS
                     RFVMPEGEHRIVKIGQQLPPEQAHGEFIGMAMFSEKGIKTFRDCYRASLDTYKSKGFH
                     EAGTLVKAGFTDLVQELIDQGHRIDAVPIFKGWMEVDSFEEYQKAWAKIRQ"
     gene            8207..8596
                     /locus_tag="VLD60_14225"
     CDS             8207..8596
                     /locus_tag="VLD60_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB46167.1"
                     /translation="MRMQPVEQPGRAAEKADHMNHRLTERVPTQFNLMYSGMHEGQML
                     IGNGMVTNLSVAGIGIRGNHIVKTGMELALFIDLPGIEEPVCVAQSRVSWVSGRRFGV
                     EMIAPKLEALNQLRFYVWNYLFRPPYR"
     gene            8628..9011
                     /locus_tag="VLD60_14230"
     CDS             8628..9011
                     /locus_tag="VLD60_14230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB46168.1"
                     /translation="MRHSRVRSAKSPHQSYQVCNRRVDSRTHAEFGLMYSAMDEHGAL
                     IMGDGMVVDLSNKGLGIRGNVPVNPGLEMTLFLYLPDGQDPLFVMEARVAWSAGRRFG
                     IVIQKMNLRERNRLRYFLSSNLYQQ"
     gene            9295..9939
                     /locus_tag="VLD60_14235"
     CDS             9295..9939
                     /locus_tag="VLD60_14235"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB46169.1"
                     /translation="MTPRSKQKRSTSQERQAGLITAAASLFAAKGFNGTTTKEIARAA
                     GVSEALVFKHFPTKRALYGAILAEKVTVNELLEAVEESAKKRDDRRVFTLIASFRIRP
                     GADSTLLRLLLFSALEGHELSEMFFGKHHRVFYDHLATYIETRSAEGAFRMVDPLLAA
                     RAFIGMVVHHRLLHEIFGVPMHRSHDETVTTYVDLFLTGLVQQPTAPAQRRGSG"
     gene            9936..11123
                     /locus_tag="VLD60_14240"
     CDS             9936..11123
                     /locus_tag="VLD60_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB46170.1"
                     /translation="MNPVKQHPIVTLGVIIFFAVTALVVFRLSSGAKVDQKKSRVITV
                     GTVTPLKQDLEIRLTYTADITPNQLVNIFSRVDGYIAKLYVDKGDFVKANQLLVEIDH
                     QDYLHAVNQAKANLAAARAKVTQQDASVRSAKLTLDRMQALIKDQFVSQQDLDNAQVN
                     SDAAIAALESLRAQVKQMEVALAQAETNLAYSYIRAPFAGYVAERNLDLGAYVTGAAT
                     SNSTTARGIVSLHDIDIVRTLIEVVEKDVPLVQIGQKAEVRAEAYPDRVFEGTVTRVV
                     QALNRATRTMTVEVDLPNKDRLLKGGMFARVEVLVGKHKNAVQIPIDAVSRLEDAQYV
                     YVVREGKAERVPVEIGVRDESRVEITKGLDGSEQVIVSGKDLVHDGTPVQTQPLAPVK
                     REG"
     gene            11210..14392
                     /locus_tag="VLD60_14245"
     CDS             11210..14392
                     /locus_tag="VLD60_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB46171.1"
                     /translation="MWLTLLALRNRIGILMLSLAMVVLGATSLNRLPVDLFPNIQVPV
                     AFVGVIYKGAPPLDIEQSVVYPIEKAVSSASNVEHVESFAKQGIGAVQIWFNWGADIN
                     VGQMEVMQRITQILNSLPPGILQPFIVKFDVSNIPVSFVTVSSDDLDERALYDLAFNT
                     IAPQIEQIANVAAATVEGGKIRQININLDPALLNARGLSILDVVKSVKAANLILPSGD
                     IKAGNLDYNVFTNNQFRTVEPIQDVIVKVNQQGSPVRVRDLGTVTDSSDIQTNIVRTD
                     GARAVYLRVNKQPVANTVEVVDALRAAIPKMIGIPPSVKLGISFDQSVYIRQSISNLI
                     EQALHGSILAAAVILIFLRNLTSTLIISVAIPLSIMVTFIVLYFTGQTLNVFTLGGLA
                     LGIGRLVDDSIVELENIQRHLNSNQRRWDAILEAAREVAMPIFASTVTTVVVFLPIFF
                     VVGIARLLLIPLTLTIAIALFTSFFVSRTVTPALCFKFLKPEQEAHPTMPRWYVRLMD
                     WSRTRYESLDRGFEDSLRWVLAHRKLFIAGIVLIFVASLALIPNIGTEFLPVSDESQF
                     RIVLRAPVGQRVEKTEQQVAEVERVIRATIPPEELETMVSSTGVLAQGRSSLFNPNTG
                     PHTSVISVYLVSPDKRKRNQVQIMNEVRPKVLKLFPGVAMFFDPGGLVKRVTSFGSQK
                     AVDVEIYGYDFEKARTVIRQVENVMHQVQGLADIEVSREENYPEVNVVVDREKAALLG
                     ISESDVANAVLFSLNGNGQTDPIIFTDPQNGNEYYISAWLAEEHRKDLTDIDNIILTA
                     KNGEPVLLKNVASLKLNAGPVKIDRKYFQRVIHITANPVNRDLGAIADDLEASFAQLQ
                     LPTGFSIKLAGQIQQQRETFSGLLFATVLALILVYMVMAAQFKSLVDPFIIMFSVPMG
                     FPGVILILFLTDTTLSTTSMMGIIMMLGIVVSNGVLLVDYTNVLRRKGRRLHDAVVTA
                     ARTRLRPILMTSLATVFGLLPMAIGLGTGGETNAPLARAVVGGLSLSTLLTLFLVPTM
                     YLILEERFPRKWEEEAEAASPTPEPAMALR"
     gene            complement(14405..14737)
                     /locus_tag="VLD60_14250"
     CDS             complement(14405..14737)
                     /locus_tag="VLD60_14250"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSB46172.1"
                     /translation="MKPISVREFFELLPGKLDADAAEDVDAVYQFDLSGAQGGQYVLT
                     VRQGLCKVTEGTHDDPHVILMMAGEDCIKVLNGQLSGQAAVMSGRIKVSGDIGLAMQL
                     PSLFPTIG"
     gene            complement(14808..15248)
                     /locus_tag="VLD60_14255"
     CDS             complement(14808..15248)
                     /locus_tag="VLD60_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HSB46173.1"
                     /translation="MSAPVGSEETILPSSAPTTSSVPAQEMVGEGKAWGLCTAVDLQD
                     CHPDLIRNADQIRRYVVELCELIGMKRFGECQVVDFGSGRVAGYSMVQLISTSLISGH
                     FANDTNSAYLDIFSCKGYDPAVVEDFSKKFFGARRSTATVTLRY"
     gene            complement(15968..16291)
                     /locus_tag="VLD60_14260"
     CDS             complement(15968..16291)
                     /locus_tag="VLD60_14260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB46174.1"
                     /translation="MGFKRKHSRIEVGRKGRIQRGSLSAACTVLDVSESGVRIESRLF
                     VKTGETLRLVIDLDHGQTLSCTIQPVYVRAPRFGARITSITESDQERLSHMFDDRIQR
                     MYSGR"
     gene            complement(16387..16755)
                     /locus_tag="VLD60_14265"
     CDS             complement(16387..16755)
                     /locus_tag="VLD60_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HSB46175.1"
                     /translation="MSPRALASCPGSPNCVSTLAPAQDTRHVIAPFSYRKSRAEAKEA
                     LKAALLILPRTKLVEEDEAYLHFEFTSLLLHFIDDVEFLFDDETKTIHFRSASRTGYS
                     DLGVNRRRMEEIRVLVDGRI"
     gene            complement(16768..17175)
                     /locus_tag="VLD60_14270"
     CDS             complement(16768..17175)
                     /locus_tag="VLD60_14270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSB46176.1"
                     /translation="MTRISGIRLLEEGEGEGRPAQKGDRVVYNTRLFLNRGDEVPLNA
                     KQAPHLPHEMIRVEEGVTFVDHSTVLGSRQAIAGVEHALTGMKVGGYRKVRISPHLAY
                     REKGIPDLIPPEAVLICEIWLRHIVPGSEGQAE"
     gene            complement(17185..18249)
                     /locus_tag="VLD60_14275"
     CDS             complement(17185..18249)
                     /locus_tag="VLD60_14275"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HSB46177.1"
                     /translation="MPAVFFLSGVIYDTLTLTRIDRLQDNLLLLIYLLLLGVLIVLTG
                     RLGIDPVPDRDQLATLSPFARWVLQSRPYYPMASQFLLGGLFSAYAIFYSRSATLTST
                     AVFFALLVVLLVGNEFLRDRLSNLRLLVSLYALVCFGFFTFFLPVITGHMNTLVFLAG
                     AGLSAVVTLRVVHLVYRNNPDRSRREAVRVTAPAFGLIGLLVGFYFLNWIPPVPLSMK
                     FGGMYHEVKKSGEHFELSYEKKWYEAWKRSDNVFPASEPIYCFTAIFAPVALKTTVYH
                     HWYFRPNSSKPFAHADKIALNISGGREGGYRAYTFKQRLDPGDWRVDVEAEDGRIIGR
                     VSLTVEDQGETQPALTTLTY"
     gene            complement(18498..19559)
                     /locus_tag="VLD60_14280"
     CDS             complement(18498..19559)
                     /locus_tag="VLD60_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46178.1"
                     /translation="MRERRWETPVPMTFGQVLAVGERLAALGLKPAVPAKDVICYVEE
                     WTVESPEDFDGLDAWATEDVTLVHVREGWRGDFFLLAGAYHTAYQRHQDIGTYCSISH
                     PWRIREPMKLHESRSMLWLGFRHAHSFLRVRLQTIEVITPGETRGDAQREQWLEERRT
                     AFLEAITTVGLPIETAIEKQQLVLRPTDPSVPFFCSWPDAFGPCQFEYNTADAYEFLV
                     PASQLAATHAPEPAGVRAYLTGFSEEALGEFQAIEPAARLAYRCSVHCPLDDLPEIKT
                     AIEPDGRLYATLCEFQTQELIPEGGDASAIIGVVASSAGFAIEARLNKAPLREELMAA
                     WLERLIGNPVIYSPLPAFV"
     gene            complement(19584..20258)
                     /locus_tag="VLD60_14285"
     CDS             complement(19584..20258)
                     /locus_tag="VLD60_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46179.1"
                     /translation="MAPTRKEGIVKKRLVRVVVGMMLLVGLLAGSGCGGTGQTFYFDV
                     IPKQQPGPLIEAEPVRIVIEPFEDRRAEKTRIGMRTHLWGGVTHFNVAGEKPGEVYAQ
                     AMAERLKAKGWNDRAWNVRVAPAGTVTDADIVISGQIFEFAANAKSRLFSTYLTTSNR
                     LTIMARNKADGSSTSRSLEGAQTDTVIWFAEEDVQKLLASTVKDTLDRYLSDTTIAQR
                     ALRPAR"
     gene            complement(20335..20955)
                     /locus_tag="VLD60_14290"
     CDS             complement(20335..20955)
                     /locus_tag="VLD60_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46180.1"
                     /translation="MNVPLSFTDDELALVADAQFFRKKARITKKIRGQLEAVHEALIS
                     ELHRATLVTPPAFDPDRVQYVKGEHLESFPYQYLDCPKHFHGSEKCTFRTLFWWGHHA
                     VFAWLLEGPLVKQYKNNLVDRFHRLAGQGLELSTAPTLWEWKRGEGYTLPITHDRKAQ
                     VAAVVAERSILKLARFVPLDDPKIGAGELADLGREAFLAMKPMLSL"
     gene            complement(21150..21527)
                     /locus_tag="VLD60_14295"
     CDS             complement(21150..21527)
                     /locus_tag="VLD60_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46181.1"
                     /translation="MAQIFRSGAFLQQCWSVHPLCIAVKRMADGNQLILTCTSCRFVH
                     QLTLASVVVRASAVLDESDNSSGDHRTARGGQDQLLGCLEAHAPALSLREMDVFEDAA
                     LVRCAECRRHYALTVSSFETHQK"
     gene            complement(21558..21893)
                     /locus_tag="VLD60_14300"
     CDS             complement(21558..21893)
                     /locus_tag="VLD60_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46182.1"
                     /translation="MTADPNSRLLQARTGWHHLLAQIIRYGLAPVILLFSGFFSANFM
                     ISGQYTWPRTSRVLALTLTLLVLSYEFVYKEQRTRTGSGEQARAAVLYSCLIPYMIGI
                     ALMFALWKF"
     gene            complement(21898..22179)
                     /locus_tag="VLD60_14305"
     CDS             complement(21898..22179)
                     /locus_tag="VLD60_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46183.1"
                     /translation="MSIRKAGGDWEPMLLGIEPRNCKVCKEGLYRNKTFLSGGWSRCT
                     VCDEFVHYSCLASGKVSFLKARPRVCKSCRAAQTGMPASSAVQADSPAA"
     gene            22244..>23174
                     /locus_tag="VLD60_14310"
     CDS             22244..>23174
                     /locus_tag="VLD60_14310"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HSB46184.1"
                     /translation="MKREKLSPFLSMAFVNVGTQSIRGLPGLTAFQTICYSPRRFRAC
                     RDNVIYSMNIKDYLEHNRIAVDRFLDEVTPPAATPPTTLHESMRYSLMAGGKRVRPIL
                     TIAAAESIGTTPPGLMAVACSLEFIHTYSLIHDDLPSMDNDDFRRGKPTNHKVYGEAM
                     AILAGDALLTMAFDLCSRPDLMKGCDAARQVRLIQELAYGSGNMGMVGGQVFDIQAEN
                     KDIDLPTLQNIHKHKTGMLIRAAVRMGAIAAGATDRQLDDVTGYAEDIGLAFQIADDV
                     LNVTGTREELGKNPNTDAERGKKTYPTFYGVDGA"
BASE COUNT         5154 a         6935 c         6393 g         4692 t
ORIGIN      
        1 atatgccaaa cgaggagact gagggtaagg agaccgactg tgagaagtat tagtctgagc
       61 acgtcgggct agggaccatc attaaaagga tgctcaaaac ggccgtccag gaaggccgcc
      121 gggagcgaag acctcgaggc gcatgttcgt cggcacctcg aggggaatga acgaccgaga
      181 acgaagctgg aggatgtttt cagcatcccg ctgctagaca cgggaaatgg ctgatggacg
      241 aagcacccag accataatca tcgcgaacac gattgatccg gccgccgcca tccaaagaaa
      301 ccagtcaagc ttccccgcga aggcaaagac cagcacgatg acggagaaat cgcggctggc
      361 gacgttcttg agcatgaaat cggaccaggc tgcatgcacc ggcgttttcc attcgctcgc
      421 gcgtttaatt ttttgcgctt tgttcacgag cgtgaaggac agggcattgc ccaatactgc
      481 ggcagcgcca agcgcgagcg gtacccaggc cgattcactc ccggcggctt ggatgaacgc
      541 cccgacggca atgccgccga agatggccat gtgcacgacg ttgtccatag caatgtcgag
      601 ccaggcgccg aagggggatt ccgtaaacgt cagccgcgcg acctctccat cgcaacaatc
      661 gattaccgcc gccaactgga agagcaaggc tgccacgatt cccgcgctgt aggtgccgag
      721 cccgaaccct gccgccgcca cgagaccgat cacagtcgcg accatggtga tcgcgttggg
      781 agagaatccg agggcgagaa agactcgcgt aaaccagcgg gaaactattc gattgaagaa
      841 acgatcgacg aaaccttcca actctccttt gagcgacgca aagagctgtc gttcggcggc
      901 ccggacatct gccgcatcac gcacagcttg gtaccacttc gaggaatcca tgccgacggt
      961 gactatctgc acgcgaccat cagccgtcgc acgttccagc caccgacgaa ccggcacaga
     1021 acctggctct ccgggtactc gtccggctgc gctgagcatg gaagcaggaa gcacgaccag
     1081 atcggcggct acggaactgc cggtttcaaa ggacgccgaa tcgaacagcg gcaatcgggt
     1141 agcaaccttc gagcggctcg gattgcccgt gccggattct cgagccacga cgatggcctg
     1201 cccttcttga acttcctgcc gcagttgttc gatcaaggga cgtgaaaaca ccccgccgac
     1261 accggacagc aggcagaacc cgcgcacttc cgccgcaagt gcttcccatg tccggggatc
     1321 gcccaacggg aattcccgaa tcggcatcca gcgaaccggg atggtgacgc gcggcccttt
     1381 tcccatcgcc tgcttcaact ggtcttcttc gggacctgcc aggacgatga gctgcctgat
     1441 cccggcgcgc tgaagggtca acacggttct cgggaacaat ccgatgccga ccacctgtgt
     1501 caatggtccg acggcctttc gcgccgacgc accgaagaga tcaacagagg gcaggagtat
     1561 ggccgtcgag agtccctgca ggtctgcttg cttatgaatg aggctctcac tcatcgttga
     1621 tcttgctttt cgtctctcac agaatcctca gcgcatcaca gcgacgcatc atctacgacg
     1681 tcgtcacaac ttcggcaaga tctcccgttc cgccttctcg acgtcttcgg gaaaatcgat
     1741 ttccgtccac ggcagcccgc cgataacttc atgaccgact ttgacctcgc ggaaaaattc
     1801 gaccagcgca tcttcgtatt ccatctgcca ggcttcccgg tccacgaagg tccgaaggga
     1861 cgagacgacg tgcggcgtat cggcttgccg gactttcaaa aacccgaccc cctcgccggc
     1921 ataatcatat cgcggcggca tcttcttcgt cagcgcaatg acgcgccctc cctcaacgac
     1981 gaccatgcac tcctcgccgg tttgcttcac cgtgtcgtcc atcaacaagg cgttgggata
     2041 gggagactgc accagcctgc gcagaatttc acgatgaaag agtacgtcgg catccatgac
     2101 gaggacgtcg tcgtcgagtg ccgtgcgcgc aatccagagc gaggaaatgc tcccgcggtg
     2161 gaactgctcg ttgaccagaa agttcacgcg gacgccgcag gcattcgcct ggaccgcggc
     2221 gcggatcatc tcctgcttgt acccgaccac gatgtcggcg cgttgaatgc ccaaactcgc
     2281 gagagcttcg agataacgat gcagcagggt tttcccgccg atttcgatca ggcacttggg
     2341 atgatgctgc gtgaccggcc aaaggcgctt gcccacgccg gcggcaagga tgatcgcttt
     2401 catgctggct tgcaggattg ttcgaacgcg tcgagaaaac tgttgaattg tgcttcggtc
     2461 agggcgccca tgttggcgac gcggaaaatc ttattttcga ggttcccttg gccggcgtag
     2521 ataacatagc cctgtccctt taatcggtca tgcagcaact gataggtgag cccttccggc
     2581 aagtagtacg ccgtgatcga attcgactgc cgttcaggcg tgagcactgg cttgattccc
     2641 atcttggcca tccgctcgcg aatcaacgtc gccatctttt tgtagcggag gatgcgcttt
     2701 gcgacgcctt cctccaagag ttcgctcagt gcttcgtcga acgcgttgta gacctggacg
     2761 gccggtgtga agggaatcgt cccttccccc tggtcgttga cgtagtgcgt gaggtgcaga
     2821 taccaggaac gcttggggta gttccgcatc cgttcgagga agcccttgcg cacgaggaca
     2881 aacgagacac cgggaaatcc ctggatgcat ttgcctgccg tgccggccac catatacata
     2941 tgggatccgg cgatatcgat ggtttcaccc gccagcgcgc tgaccgcatc cacaataaac
     3001 acacggtttt ggctatcgac gacctcggca atgtctttga ccgggttgag caatccggtc
     3061 gtcgtttcat ggtgaaccat cgccacaccg tgcacttccg gatgttgtct cagagccaga
     3121 cgcaaccgct cgggatcagg cttgaccgtc cagtcatact tgagttcgga aacgcccaac
     3181 cgatggagac cgaccatttg cgagagccgc tcgccgtaga cgccgttatt aatgataaga
     3241 atgcggcggc cgtgcgggat ggaggacatg agcgccgact cgaccgcggc agtcccggag
     3301 cccgtgatga tcacggcggc gtagtccgct tccgcaccgg gcacgaaggc tttgagcagc
     3361 ttctctttga tacgttgcag cagttccgta aattcggatt cgcggtgaca gatatcaggc
     3421 cgcaagagcg cctggcgtac tcgctccgtt acgttgacag gacccgggtt cagcaacatc
     3481 ataggtatta ctcaatcgct ttcatgaaac gttttgtgat gtccggcgga tcatgcagca
     3541 cccgaccggc gtcttcagca aactcgttca ccttgatcaa taacatgctg gggccgtcct
     3601 tcttcagcat gtctttgaac tcatagacca gatcatcgcg atcgagcacc cgctcaacat
     3661 gcacgtagcc cgcggccttg gccaccttct ccagcggaac cacattggag atcgtcggtt
     3721 ggttccccgt cgttccgtag acttcgttgt cgaagaccac atggataaaa ttcttcggtt
     3781 tcagcgcgcc gacggtcgcg agcgtcccca tgcccatcaa cacgttgccg tccccatcga
     3841 agacgaccac ctgcttgttc ggtttggtga gcgcgacgcc gagcgcaata gcgggggcat
     3901 tccccattga tccgatcatg tagaaatgag tgggccgatc ggcgatcttg tgtgcctcgc
     3961 gggaggggaa cccgttgcaa atcacgaccg gctggtccgt aagcagctcc agcaaggcgg
     4021 cgatggcttg tgcgcggctg atcaacgtgc cctgttccgg tctcatggat gcaatccttt
     4081 cacgacgccc tttgtaatga acaacgcgac cgggatccgt tgcttcataa aggtctccgc
     4141 cacccacttg aaatcatcca cggccgtctt ctcggtcaac gtccggtgcg gaatcttcat
     4201 cgtgtcaaga aactgcggca tgacctcacc catcacgaga tgctcgggcg cgtccttccc
     4261 cccctggccg cgccaggaca caatgaggat acagggctgc cgataaatca tgttcagcga
     4321 aatcaaggta ttaagcgagg tgccgagccc ggagttctgc atcagcaccg ccgggacttt
     4381 tcccgccata tatgcgccgg ccgccatggc gactgcctca tcctcccgaa cagccggagt
     4441 atagaggcga cggtccatga gttccgcaat aatccctccc aggatcgaat cgggcacccc
     4501 cgtaaagaag ttcaccccca tatcctgcat ggcctgcaca aaaacatcgc tgtcgatcat
     4561 ggttaccgtc ttccttcgat caatacgtcg gtcgtagaaa tgcgcggcgc attataagcc
     4621 atgctccgat acgttctcaa ggaaacagcg tcctcgactt ttcggtaacc agtcagttgc
     4681 gcctcgcatt gcggcatggt agcgtgccac catgcgatgg tttctcgcat ggcttggcct
     4741 gtttctcttc ctcctccagc cgtccgtctc tcgcgccgtt ccgatgtcga acgatcccgg
     4801 cggttttcac agtatcccct ggggagtttc gctgaaatcc aggcaggatg tagagatcac
     4861 gcgggaaggc ccccacatta ttgagtatca attgaaagat gctgcgcctg tatttgccga
     4921 cattcaggtg gacagcgttc gccttgcaac cgtggatgag caattcgccc gcgtcacgat
     4981 ccgctaccat ggcgatggga cacataagca gatcatgacc tatctcgaac ggcagttcgg
     5041 ccagatcgag cgtcttccgg ggcagatgat gcgcggcctc aatcagcagt ataattggcg
     5101 gggcagcgat tcggagatca atctgaccta tcaggctaat accgagcgcg gattcatttt
     5161 tctcgacagc cggacgttgg cccctcgatt taacgatcac attgccgact cggcggaata
     5221 gcgcgatgac gtatcaggca acctctggac cttttcaact gtggtcgttc ctggcaggca
     5281 gcatccttgc cgtcctcctt atgtgcaacg ccgtcctcgc agtcccgatg atagaagatc
     5341 ctaacggatt tgaagggatt ccctggggag ccacgttgtc cgaatccgag accttcggaa
     5401 aggttgagga cgccggccgt ttcaaaacct acgagctcaa ggccagccca ccgacgctcg
     5461 gaccgactaa ggtcgaatcg atgaaattca tcacgatcga cgacaggttt gcgcgcgtga
     5521 cggtgcgcta tcagggtaag gaagcacatg aaaagatttt ggcatggcta caaacccgct
     5581 acgggccgct cgatcgcacc ccagggcaat tttcagtcgg accggtgaag ttctacgctt
     5641 ggcagggttt cgaaacagaa gtcaccatgc gttacgaagc gcagatggac cgcggcatta
     5701 tctttttcga gagtcagact ctgcgcaata aggtatcgga cggcaattcc cccacggtct
     5761 tttagccaca ttcagccggt tcctgaaact tttcattgac aaggcgccat gcggtttcgc
     5821 atcatgcgac cttatgcatc aagccactaa gcttcgcgcc ctgctgaaaa agcccggggc
     5881 aatcaagatc gtcggagcgc atgacgcgct cagtgccaag ctcatcgagc gagccggctt
     5941 cgacggagta tgggccagcg gtttcgcgat ttcggcctca ctcaagtgca tccccgatgc
     6001 gagcttcatc gactccagcg agcaattggg cgtggagcgc cggatggcgg aagccattga
     6061 tattcccatt atcgccgatt gcgacaccgg ctacggcaac gcgctgaatg tcatgcggac
     6121 ggtcaatgac cgcgaacggg ccggagtcgc ggccatttgc atcgaagaca acgtctatcc
     6181 caagcgctgc agcttctatg caggtgtgcg ccgggaattg attccgatcg acgagcattg
     6241 cggaaagatc aaagcggcga aggccgcgca aacagtgccg gacttcatga tcatcgcccg
     6301 gaccgaagcg ttgatcgccg gatggggcaa agaagaagcg ctcagacgag cggaggccta
     6361 cgccgaagcc ggtgccgatg cggtgctcat ccattccaaa tccaaaacct tcgacgagct
     6421 caagtcggtc tacaaggcct tctccggccg cgtgccgatt gtcgtggtcc caaccatttt
     6481 cgaccagacg accgcggcgg aaatggaaga cgccggcgtc aagatcatca tctatgcgaa
     6541 tcaaccggta cgcgcggcga tccgcggcat gcgcgagaca ctcgagctaa tcaagacgga
     6601 cacgcgtcct ggcgccgcga acgaccgcat cgtgacgctc cccgaggtgt acgacatcgt
     6661 cggcgttcca cagatggaga gagacgaaca agcctttctc cccgtcggca gcgagaagat
     6721 taccgccgtc attgccgccg ccggatttga aaagcaattg ctgcccttga ttgaagacaa
     6781 gccaaaatgt ctgctcgata ttaagggcaa gaccatcctc gaacgccagg tcgccgcgtt
     6841 gaacgagtgc aacatcaagg aaattgccct cgtcaggggc tacaagaaag aggcgatcac
     6901 gctcccgaac atccggtact acgacaacga tcgctacgaa gaaaccggcg agctgttttc
     6961 gatcttttgc gcggaaaccg agatgaaggg ccggaccatt cttctgtacg gcgacatcat
     7021 cttcgacaat acgatcctcg agaagctgct caagagtccg gccgatatct cgttggtagt
     7081 ggatctggcc tggcacgacc agcagcatct cggcgcgcag ccggcgcatc tcaatccgga
     7141 cctcgtctcc ttagccgaac ctcccggcaa gagttatcac tcgcggttcg taatgccgga
     7201 gggcgagcac cggatcgtga aaatcggcca acagttgccg cccgagcagg ctcatggcga
     7261 attcatcggc atggccatgt tctccgaaaa aggtatcaag acgttccgcg actgttatcg
     7321 ggcgtccctc gatacgtata aatccaaggg gttccatgag gccggcacgc tggtcaaagc
     7381 tgggttcacg gacctcgttc aggaactcat cgatcagggc caccggatcg atgccgtgcc
     7441 aatcttcaaa ggctggatgg aagtggactc cttcgaggag tatcagaaag cctgggccaa
     7501 gatccgccag taagcaccaa gtaccaccac gtaatcccca tcgttccggc caaacaccgc
     7561 ccaaagtagg tccttgctgc gcctcatgac cggtctcaat tcattgtgct cctggctgat
     7621 ccccctcttc tggtggggat caaacccctc tctttaggaa tggtcgaagc gtcagactgc
     7681 ggtagtctca agttgttgcg agggcccaat gccagcgatc gaacgcaggg aggcaaccac
     7741 gatgaaaacc agcgtccttg actacaatct cacctcaaaa agcgctcacg ccccattgga
     7801 ccgacgatac gccgagcgcg tgcccatcaa ctatcgagtc acctatacag gggcggaagg
     7861 cgctaggcta gtcaagacgg aaggatcatt acgggatctg tccaagactg gatgtaaggt
     7921 cttggggacg attctgcccg tcacgggcag cacagtgact ctcacgcttt atctggaaga
     7981 tgggcaggca cccttatgcc taagcgatgc cacagtctcc tggatcaggg gcagtgtctt
     8041 tgctattcga ttcccgaagc tgactgccaa cgagcggaag cgcattcagg aagttatctg
     8101 gaggcacgtc accttatctc catccaagaa tcaccgagcg gcattccgca ttgtttccta
     8161 agaatgaatc aaggagcggt agcgtcatca ttttggagga caggcgatga gaatgcagcc
     8221 cgtcgagcag cccggtcgag cagcggagaa agcggaccac atgaatcatc gactgacaga
     8281 acgggtaccg acccagttca atctgatgta ctccggcatg cacgagggac agatgctgat
     8341 cggcaatggg atggtgacca atctgtctgt ggcaggaatc ggcatcaggg gaaaccacat
     8401 cgtcaagaca ggaatggagc tggcgctctt tatcgacttg cccggcatcg aggaaccggt
     8461 ttgtgttgcg cagagccgcg tttcctgggt ctcgggacgc cgcttcggcg tcgagatgat
     8521 cgcccccaag ctcgaagcgc tgaaccaact gcggttttac gtgtggaatt acttattccg
     8581 tccgccgtac aggtaaccct gcccaagaga tcaaaggagg aaagaccatg agacactcca
     8641 gagtccgctc cgcgaaatcg ccgcaccaga gttaccaggt ctgcaaccgc agagtcgatt
     8701 cccgcaccca cgccgagttc gggttgatgt attcggccat ggacgaacac ggggcactga
     8761 tcatgggtga cggcatggtt gtcgatttgt cgaacaaggg cttgggtatc cgcggcaacg
     8821 tgccggtcaa cccgggattg gagatgaccc tgtttctgta cctgcccgat ggccaagacc
     8881 ccctctttgt gatggaggcc cgcgtcgcct ggtcggccgg caggcgattc ggtatagtga
     8941 ttcagaagat gaacctccgt gaacggaatc ggcttcggta cttcttgagc tcgaatctct
     9001 atcagcaata gggttataat cggtattggg aaggacgcag accctcgaca caaaccaagt
     9061 acctgagcgc acttatgtga ttgatacgcc gcagttcctg ctacgggcca gctgcgagca
     9121 cctcccactc ccccgattgc ccatcacatc ggttttgacc tcaggcccca acaaccctct
     9181 cttgtttttc ttctgatatt tttccccttc cgaaaaccaa cccttcattg tcagagcaaa
     9241 tggattcatg atagcctcaa cggtgagtaa ttactcactc acttatttac gctcatgacg
     9301 ccacgatcca aacagaaacg ctcaaccagc caagaacgcc aggcaggcct gatcaccgcg
     9361 gctgcgtcgc tcttcgcggc caaaggattc aacggaacga cgacgaaaga aatcgccagg
     9421 gccgccggag tgagcgaagc cctggtgttt aagcatttcc cgaccaaacg cgcgctctac
     9481 ggggcgatcc tcgccgaaaa agtcactgtc aatgaactct tggaagctgt cgaagaatca
     9541 gccaagaagc gggacgaccg ccgggttttt accctgatcg ccagcttccg gatccgaccc
     9601 ggagcggatt caactctcct gcgattgctg ctgttcagcg cgctcgaggg tcatgaactc
     9661 tccgagatgt tcttcggcaa gcaccatcgg gtgttttacg atcacctggc cacgtatatc
     9721 gaaacaagaa gcgccgaggg ggcgttccgg atggttgatc ccctccttgc ggcccgcgcc
     9781 ttcatcggca tggtcgtgca tcaccgccta ctccatgaga ttttcggcgt accaatgcac
     9841 cgatcccacg acgaaacggt gacgacatat gtcgatcttt tccttaccgg actcgtccaa
     9901 cagccgacag cgcctgcgca gcggaggggt tcaggatgaa tccggtcaag caacacccca
     9961 tcgtgaccct cggagtcatc atcttctttg ccgtgacggc gctggttgtc tttcgtctca
    10021 gcagcggggc gaaggtcgat caaaaaaaat cgcgggtcat cacggtcgga acggttacgc
    10081 cgttaaaaca ggacctcgag atccgcctca cgtacaccgc cgatattacg cccaatcaac
    10141 tggtcaacat tttctctcgt gtcgacggtt atatcgcaaa gctgtatgtc gacaagggag
    10201 attttgtgaa ggccaatcaa ttgcttgtgg agatcgacca ccaggattat ctccatgcgg
    10261 tcaaccaggc caaagccaac ttggcggcgg cccgcgccaa ggttacccag caggacgctt
    10321 ccgtacgcag cgcgaagttg accctcgatc ggatgcaggc cctgattaaa gaccagttcg
    10381 tctcgcagca agacctggac aacgcccagg tcaattcgga tgccgccatc gccgcgctgg
    10441 aatcgctcag agcgcaggtc aaacagatgg aggtggccct ggctcaagca gaaaccaatc
    10501 tggcctactc ctacattcgc gcgcccttcg cggggtacgt cgccgagcgg aacctcgatc
    10561 ttggcgctta cgtcaccgga gccgcgacca gcaactcgac gaccgcccgt ggcattgtaa
    10621 gcctgcacga tatcgacatc gtccgcacgc tcatcgaagt agtcgaaaag gatgtgccgt
    10681 tagtgcagat cgggcagaag gcggaggtcc gtgccgaagc atacccagac cgtgtcttcg
    10741 aaggaacggt cacccgagtc gttcaagcct tgaaccgcgc gacccgcacc atgacggtcg
    10801 aagtagatct ccctaacaag gaccgcctgt tgaaaggcgg gatgtttgcg cgagtcgagg
    10861 tgttggtggg caaacacaag aatgcagtgc agatcccgat cgatgccgtc agccggctgg
    10921 aggatgccca gtacgtctat gtcgttcggg aggggaaagc cgaacgagtc ccggtcgaaa
    10981 tcggtgtgcg cgatgaaagc cgcgtcgaaa tcaccaaggg attggatggc tccgaacagg
    11041 tcatcgtctc cggcaaagac ctcgtccacg acggcacgcc ggtgcaaacg caaccgctcg
    11101 cgccggtgaa acgtgaaggg taagggatga acaggcctat ttcccagcgc ttctaatcca
    11161 agagatcgtt tttcattttt tacaccttat ccctcgctct tgattgatta tgtggttgac
    11221 cctcctggca ctcagaaacc gcatcggcat attgatgctg tcgctcgcca tggtcgtcct
    11281 cggcgcgaca tctctcaatc gcctgccggt cgacctgttt ccaaacatcc aggttcccgt
    11341 ggccttcgtc ggcgtcattt ataaaggtgc cccgccgctt gatatcgaac aaagcgtcgt
    11401 ctatcccatc gagaaagcgg tgagttccgc ctccaacgtg gagcatgtgg aatcgttcgc
    11461 gaagcaaggc atcggcgccg tgcagatctg gttcaactgg ggcgcagaca tcaatgtcgg
    11521 ccagatggag gtgatgcagc gcattacgca gatcctcaac agccttccgc ctggcatcct
    11581 ccagcccttt atcgtcaagt tcgacgtgtc gaacattccc gtatcctttg tcacagtttc
    11641 gagcgacgac ctggatgaac gagccctcta tgacctggcc tttaacacga tcgctccgca
    11701 gatcgagcag atcgccaatg tcgccgccgc cacggttgaa ggaggcaaaa tccgccagat
    11761 caacatcaac ctcgacccgg cactgctgaa cgcaagaggc ctctcgattc tcgacgtggt
    11821 gaagtcggtg aaggcggcaa atctgattct gccctcgggc gacatcaagg ccggcaacct
    11881 cgactacaac gtcttcacca acaatcagtt ccgcaccgtc gagccgattc aagacgtcat
    11941 cgtcaaggtc aatcaacaag gaagcccggt ccgcgtaaga gatctcggca ccgtcaccga
    12001 ttcctccgat attcagacca atatcgtccg cacggatgga gcgcgagccg tgtatctgcg
    12061 ggtgaacaag cagcccgtcg caaataccgt cgaggtggtt gacgcattgc gcgcggccat
    12121 tcccaaaatg atcggcatcc cgcccagcgt caaacttggc atctcgttcg atcaatcggt
    12181 gtacattcgc cagtcgatca gcaacctcat cgaacaggcc ttgcacggtt caatcctggc
    12241 cgcagcggtc attctgatct tcctcagaaa cctcacgagc accctgatca tttcggtcgc
    12301 catccccctc tcgatcatgg tgaccttcat cgtgctctac ttcacgggcc agaccttaaa
    12361 tgtgttcacg ttgggaggac tggcgctcgg catcggtcga ttggtcgacg attcgatcgt
    12421 cgaattggaa aacatccaac gccacctcaa ttcgaaccaa cggcgttggg atgcgattct
    12481 cgaagccgcc cgggaagtgg ccatgccgat ttttgcctcg acggtcacca cggtcgtcgt
    12541 cttcctgccg atcttcttcg tcgtcgggat cgcccgctta ctcctgatac cgttgacgct
    12601 gacgatcgcg atcgcgctct tcacctcgtt cttcgtgtcg cgcaccgtca ccccggcgct
    12661 ttgtttcaag tttctcaaac cggaacagga ggcgcacccc acgatgcccc gttggtatgt
    12721 acgtctcatg gattggagcc gtacgcgcta cgagtcgctg gatcgcggat ttgaagactc
    12781 gcttcgttgg gtcctcgccc atcgcaaact gtttatcgca gggatcgtgc tcatcttcgt
    12841 tgcctcgctc gcgttgattc caaatatcgg gacagagttt ctgccggtct ctgacgaaag
    12901 ccagttccgg atcgtcctgc gggcccccgt cggccagcgc gtggagaaaa ctgagcagca
    12961 ggtcgccgag gtcgagcggg tgatccgggc gacgatcccg ccggaagaat tggaaacgat
    13021 ggtgtcgagt accggcgtgt tggctcaggg ccgctcctcc ttatttaatc ccaacacagg
    13081 tccccacacc tctgtcattt ccgtctacct tgtttcgccg gataagcgga aacggaacca
    13141 ggtgcagatc atgaacgagg ttcgtccgaa agtcctgaag cttttcccgg gggtggccat
    13201 gttcttcgat ccgggcggtc tggtgaagcg cgtaaccagc ttcggctcgc aaaaagccgt
    13261 ggacgtggaa atctatggct atgatttcga gaaggcccga accgtcatcc gacaggttga
    13321 gaacgtaatg caccaggtcc agggcctggc tgacattgag gtcagccgcg aggagaatta
    13381 ccctgaagtc aatgtggtcg tcgatcgtga aaaagccgcc ctgctcggaa tcagcgaatc
    13441 ggacgtggcc aatgccgtac tcttttcact caacggcaat ggccagaccg atccgatcat
    13501 attcaccgat ccgcagaacg gcaacgagta ctacatcagc gcctggctgg ccgaggaaca
    13561 ccgcaaggat ttgaccgata tcgacaacat catcctgacg gcgaaaaacg gcgagcccgt
    13621 cctgctcaag aacgtggcat cgctgaaatt aaatgccggc ccggtaaaga tcgaccgcaa
    13681 atacttccag cgggtcattc acattacggc gaatcccgtc aaccgcgatc tcggcgccat
    13741 cgccgacgac ctcgaagcct catttgccca gcttcagctg ccgacaggct tcagcatcaa
    13801 gttggccggt caaattcagc agcaacgcga aaccttcagt ggccttctct tcgccaccgt
    13861 cctggcgctg atcctggtct atatggtgat ggccgcgcag tttaagtcgc tcgtcgaccc
    13921 cttcatcatc atgttctcgg tcccgatggg cttccccggc gtaatcctga tcctgttttt
    13981 gaccgatacc accctgtcga ccacgtcgat gatgggcatc attatgatgc tcggcatcgt
    14041 cgtctcgaac ggcgtgctgc tggtcgacta caccaacgtc ctgcgacgaa aggggcgacg
    14101 gctccatgat gcggtggtaa cggcagcgcg cacgagactc cggcctatct tgatgacctc
    14161 actcgccacg gtgttcggtt tgctacccat ggcgatcggc ttggggaccg gcggagagac
    14221 gaacgcgcct ctggcgcgag cagtcgtagg cgggctgagc ctctcaacgc tcttgaccct
    14281 ctttctcgtg ccgacaatgt atctgatctt ggaagagcga tttccaagga agtgggaaga
    14341 ggaagctgag gcggcgtcac caacgcccga accggccatg gccctgcgct aaactcccga
    14401 ccgttcagcc gatcgtcgga aacaacgacg gtagctgcat cgccaggccg atatcgccgc
    14461 tgaccttgat tcgacccgac atgaccgccg cctggccact gagctggccg ttcaatacct
    14521 tgatgcaatc ttctcccgcc atcattaaga tgacgtgcgg atcgtcgtgc gtaccttccg
    14581 ttaccttgca cagtccttga cgaaccgtca ggacgtactg ccccccctgc gccccgctca
    14641 agtcgaactg gtacaccgca tcgacatctt cggcagcatc ggcatcgagc ttgccgggca
    14701 gcagctcgaa gaactctctg acgctgatcg gtttcatgat ctggtgtgga cagcggatgg
    14761 gatagggacc gggtgccgcg tgcccgcggc actcggtcga ggcacgatta atagcgaagc
    14821 gtcacggttg cagtcgatcg tctggcgccg aagaacttct tcgagaaatc ttcgacgacg
    14881 gcagggtcat agcccttgca gctgaaaatg tccagatacg cgctgttcgt gtcattggca
    14941 aaatggccgc tgatcagcga agtcgagatc agctggacca tggaatagcc ggcgacgcgt
    15001 cctgagccga aatcaacgac ctggcattcg ccgaatcgct tcatgccgat gagctcgcag
    15061 agctccacga catagcggcg aatttggtcg gcattgcgga tcaggtcggg atgacaatct
    15121 tggaggtcaa cagcagtaca gagtccccac gctttcccct ctcccaccat ctcctgagcc
    15181 gggacagagg aagtcgttgg ggcactcgat ggcaaaatcg tctcttcgga accaaccggg
    15241 gcgctcatat ggcgtccgcc tttctgtgtg agggtgaaaa accaactgcg tggatttttt
    15301 tgcactataa cacgattctt tccagatgct atggggaagc gaaaaaaagt tttgccctct
    15361 ggttccggcg ccgctcgatc ggttgacaag tttcgcgacc ctcggtgaca atgcgcccat
    15421 gacggcgccg ccatcacatc ccgggccatc gtccgccgac cccccggacc ggctctgcac
    15481 ctggtgcaag gtgccgatga agaaacggct cgtggcgagc ggccagttcg tccattacac
    15541 ctgccctaaa tgcgtcttcc agcacacgac caggctgggt cctaagccgt catcgccggc
    15601 ccattgaacg gcataccgcc tcgcgccgcc atctggcgca tcggcttttt aagcagcctc
    15661 ttcctccttc ttcctgcctg tcaatccgtc ccgaccaaga cctatgagaa tgagtcgcga
    15721 acgagaacgc aagggcacag gctcagcttg cactgaagaa gatgacgaag ttcatggatt
    15781 tcccgaaggg aaaagagttc cagcaaacta acacgatctt ttagtggtac gaatttgaag
    15841 aattcgaagc ctactgaaat ctgggctatc tgatgagctg agcttacctg tcgaacctgc
    15901 agtttactaa cgcagaactg gtcccctgtc tcggcctgtc aagcgctata gacagatact
    15961 tgaaacgcta ccgcccagag tacatacgct gaatgcggtc atcgaacatg tgggagagcc
    16021 gctcttggtc actttcggtg atcgaggtga tccgagcacc aaatcgagga gcgcgtacat
    16081 aaaccggctg aatcgtacag gagagcgttt ggccgtgatc gagatcaatg acaagccgta
    16141 gggtctcgcc cgtcttgacg aacagccggc tttcgatccg cacaccggac tcactgacgt
    16201 ccagaaccgt gcaggccgca gagagggaac cgcgctggat gcgacccttc cggccaacct
    16261 caatgcgaga gtgttttcgt ttgaatccca cgagtgctcc gcttactgag acaccatagc
    16321 aagggaatca cctaactgcc aggtaaatgc tcgaatcctg ccggaaggga acaatcccct
    16381 gagaggctag atcctgccat ccaccaaaac gcggatttct tccattcgcc gacgattcac
    16441 ccccagatcg ctgtaaccgg ttcgggaggc ggaacggaaa tggatagttt tggtctcgtc
    16501 atcgaagagg aactcaacat cgtcaataaa atgtaagagc aggctggtga attcgaagtg
    16561 cagataggcc tcgtcttcct cgacgagttt cgtgcgcggc aatatcaaga gtgcggcttt
    16621 caacgcctcc ttcgcctcag cccgcgactt ccgataggag aacggcgcga tgacatgccg
    16681 ggtatcctgc gccggcgcca atgtcgacac gcagttgggg ctgccgggac aagaagctaa
    16741 cgctctcgga ctcacctgcc ctccgtttca ttcagcctgt ccttcagacc caggcacaat
    16801 gtggcgtaac cagatctcgc aaatcaacac cgcctcggga ggaataaggt ccggtatgcc
    16861 cttttctcga taggcgaggt gtggactgat gcggaccttg cgatacccac ccactttcat
    16921 gcccgttaac gcgtgttcga cgccggcgat cgcctggcga cttcccagga cggtcgagtg
    16981 atcgacgaag gtgacgcctt cttcgactcg aatcatctca tgcggaagat gcggcgcttg
    17041 cttagcattc aacggaacct catcgcccct attcaagaac agtcttgtgt tgtagacgac
    17101 gcggtccccc ttttgcgccg gtcgcccctc accttcccct tcctcgagca acctgatgcc
    17161 gctgattcgt gtcattggat gaactcagta cgtgagcgtc gtcaacgccg gctgggtctc
    17221 gccctggtcc tcgactgtga gcgacacgcg tccgatgatc cggccgtctt ctgcttcgac
    17281 atccacacgc caatcgccgg gatcgagccg ttgcttgaaa gtgtaggctc ggtagccacc
    17341 ttcgcgtccg ccggagatgt tgagcgcgat cttgtcagca tgcgcaaagg gcttgctgct
    17401 gttcggccga aaataccaat ggtgatacac ggtcgtcttc aaggcgaccg gcgcaaagat
    17461 cgccgtgaag cagtagatcg gctcgctggc cgggaacacg ttgtcggagc gcttccaggc
    17521 ctcgtaccat ttcttttcat aggaaagctc gaaatgctcg ccgctttttt ttacctcgtg
    17581 atacatcccg ccgaatttca tcgagagcgg cacgggagga atccaattga gaaagtagaa
    17641 gccgaccagc agaccgatca atccaaaagc gggagcggtg acgcggacgg cttcccggcg
    17701 cgatcgatcc ggattgttgc gataaaccag atgcacgacg cgcaaggtca ccaccgcgct
    17761 gagcccggcc cctgccagaa acaccagcgt attcatgtgg ccggtaatca ctggcaggaa
    17821 gaacgtgaag aacccaaaac agaccagggc atacagactc accaacaggc gcaggttcga
    17881 cagccggtcc cggagaaatt cgttgcccac caacaggacg accagcaacg cgaagaagac
    17941 ggccgtgctg gtgagcgtcg cgctgcggga ataaaaaatc gcgtaggcgc tgaacagccc
    18001 tccgagcaga aactggctag ccatcggata gtagggccgg ctctgaagca cccagcgggc
    18061 gaagggcgac agggtggcca attgatcgcg atcgggtact ggatcgatgc caagccggcc
    18121 ggtcaacacg atcagcactc ccaagagcag cagatagatt agtagaagga ggttatcttg
    18181 aagtcgatca atccgcgtga gcgtcagggt gtcgtagatc actccggaga gaaagaacac
    18241 cgccggcatg aacggcttcg caagaactga ttggactctg gcaaacgggc tcatggtccc
    18301 actgtgcgga atgccgagat cttgatcaac taaaaatagg attcggacga tctgaccaac
    18361 aaaccagtcc catacagaat gttcaaaagg ccatccggca aggccgcagc gagcgaaaat
    18421 ctgaggcgta cggttttagg tacgttgagg gtttgagcga tgcgagaacg atgccggtgg
    18481 gctttttcaa cattctgtca gacaaacgcg ggcagcgggg aatatatcac cggatttccg
    18541 atcagccgtt ccaaccacgc ggccatcagt tcttcgcgaa gcggcgcctt attcaatcgg
    18601 gcttcgatgg caaaaccggc gctggatgcg accacaccga tgatggccga ggcgtccccc
    18661 ccttccggaa tcaactcctg ggtttggaac tcgcacaggg tggcatagag acgtccgtcc
    18721 ggttcgatcg ccgttttgat ttccggcaga tcgtccagcg ggcaatgaac cgaacagcga
    18781 taggccagcc tggccgccgg ttcgatcgcc tgaaattcgc cgagggcctc ctctgaaaag
    18841 ccggtcagat aagcgcgcac tcccgccggc tccggcgcat gggtcgccgc gagttggctg
    18901 gcaggaacga gaaactcata ggcatctgcc gtgttgtact caaattggca gggaccgaac
    18961 gcatcgggcc aagagcaaaa gaacggaacc gagggatcgg tggggcggag taccaactgc
    19021 tgtttttcaa tggcggtctc gatcggcagc cccacggtcg taatggcctc aagaaacgcg
    19081 gtgcgccgtt cctccagcca ttgctcccgt tgagcatccc ctcgcgtctc gccgggagtg
    19141 atgacttcta tggtttgcaa tcgcacccga agaaacgaat gggcatggcg aaagccgagc
    19201 cagagcatgc tccggctctc gtgcagtttc atgggttcgc gaatacgcca gggatggctg
    19261 atcgagcagt aggtaccgat gtcctgatgg cgttggtacg cggtgtgata cgctccggcg
    19321 aggagaaaga aatccccgcg ccagccttcc cgcacatgaa ccagtgtcac gtcctcggtc
    19381 gcccaggcgt caagaccgtc aaaatcctca ggcgactcga cggtccattc ttcgacgtaa
    19441 cagatgacat ccttagccgg cacagccggc ttgagcccca aagccgccag cctttcgcca
    19501 accgccagca cctgtccaaa cgtcatcggc acgggagttt cccaccggcg ctcacgcaca
    19561 gactctgcct ccggaatggt ctattaccgg gccggccgca acgctcgctg agctatcgtg
    19621 gtgtccgaga gatagcggtc gagcgtgtct ttcaccgtgg aggccagcaa cttctggacg
    19681 tcctcttcgg caaaccagat cacggtatcg gtttgcgccc cttccaaact ccggctcgtg
    19741 ctgctgccat ccgccttgtt ccgcgccata atggtaagcc gattgctcgt cgtcaaatag
    19801 gtcgagaaga gtcggctctt cgcattggcg gcaaactcga aaatctgccc gctgatcaca
    19861 atatccgcgt ccgtgacggt gccggccggc gccacccgca cattccaggc tcgatcgttc
    19921 cagcctttgg ccttcaaccg ctcggccatc gcttgagcgt agacctcacc gggtttttcg
    19981 ccggctacat tgaaatgggt cactccaccc cacaggtgcg tccgcatccc gatgcgggtt
    20041 ttctccgccc tacgatcttc aaacggctca atcacaatcc tgacgggctc cgcctcaatc
    20101 aatggccccg gctgctgctt gggaatcaca tcaaaataaa acgtctgtcc ggttcccccg
    20161 cagccggatc ccgccaacag tccgaccaac aacatcattc ctacgactac gcgtaccagc
    20221 cgtttcttca cgattccctc cttacgggtc ggcgccatca caataacgag cctcctccat
    20281 tgaacaacgc gcgctagtct accgaaatcc ttgtgccttc acaattgagg aaattcagag
    20341 cgacagcatc ggcttcatcg ccaggaatgc ttctcgcccg agatctgcaa gttctcccgc
    20401 cccaatcttc ggatcatcca gagggacaaa acgggccagc ttgaggatcg atcgttccgc
    20461 cacaaccgca gctacctgtg ccttccgatc gtgggtgatc ggcaaggtat agccttcgcc
    20521 gcgcttccat tcccagagcg tcggagccgt ggacaattcc agcccttgtc cggccaggcg
    20581 atggaaccga tcaaccagat tattcttgta ctgtttcacg agcggcccct cgaggagcca
    20641 ggcaaagacc gcatggtgtc cccaccagaa gagggtgcgg aacgtgcact tctcactccc
    20701 gtgaaaatgc ttgggacaat cgaggtactg gtatggaaac gactccagat gctcgccctt
    20761 cacatattgc acacgatccg ggtcgaaagc gggcggagtc acgagcgtgg ctcggtggag
    20821 ttctgatatc aaggcttcgt ggacagcctc cagctggccg cggatcttct tcgtaatcct
    20881 ggccttcttg cggaaaaatt gcgcatcggc aaccagagcg agttcgtcat ccgtaaatga
    20941 aagtggaacg ttcatgatgg ggagactttt ctctggtcga ggaaccaacc aagaaatacg
    21001 ctatatctca gattgtcctt ggggttggag aacggcgaga acgcccttcc agcaaggccg
    21061 cagcaagcga aaggctgagg cgtactccac accgttcgtt gaagccttaa gcgatgcgag
    21121 aacgcaactg gagggcctta gcgacatgtt catttctggt gtgtttcaaa ggacgacacc
    21181 gtcaatgcat agtgccggcg gcattccgcg cagcgcacca gtgccgcgtc ctcaaataca
    21241 tccatctcgc gcaacgacaa cgcaggagca tgggcctcaa ggcaaccgag gagctgatcc
    21301 tgcccacccc gcgcggtcct atgatcgcct gatgagttgt ccgactcgtc caacaccgcc
    21361 gaggcccgga ccacgacgga agccagggtc agttgatgca cgaaccggca agacgtgcag
    21421 gtgagaatca gctggttgcc gtcagccatc cgcttgacgg ctatacaaag tggatgaacc
    21481 gaccaacatt gctggagaaa agccccgctg cgaaagatct gcgccattgt ccttcctctc
    21541 gagcagagcg agctcgacta gaacttccag agcgcaaaca tgagagcgat accaatcata
    21601 taggggatga gacatgaata gagcaccgcc gcgcgggcct gctctcccga accggttctg
    21661 gtccgctgct ctttgtagac aaactcatac gaaaggacga gcagcgtcag tgtgagcgcc
    21721 aggactcggc tggtgcgcgg ccacgtgtat tggccgctga tcatgaagtt ggctgaaaag
    21781 aatccgctga acaggaggat caccggcgcg agcccatagc ggatgatttg cgccaacaga
    21841 tgatgccaac cggtgcgggc ctgaaggagc ctggaattgg gatcggctgt cacgagccta
    21901 ggctgccgga ctgtccgcct ggacagcaga ggaggccggc atccctgttt gggcagcgcg
    21961 acagctcttg cagacacgag gccgggcctt gaggaacgac accttcccgc tggccaggca
    22021 actgtagtgg acgaattcgt cgcacaccgt gcaacgggac cagccacctg agaggaacgt
    22081 cttgttgcgg tagagccctt ccttgcagac cttgcagttt cggggctcaa tccccaacag
    22141 catcggctcc cagtcaccgc cggcttttct aatgctcatc gtgcagggag tatagcgaag
    22201 cgtccgccga aaatacaaga agccggatca cggatcggca gcagtgaaac gagaaaaact
    22261 ctcgccgttt ctgtctatgg cattcgtcaa tgtcgggaca cagagcatcc gaggtcttcc
    22321 cggtttgaca gccttccaga ccatttgtta ctctccgcgc cggttcagag cctgtcgcga
    22381 caacgtcatt tattccatga atatcaagga ctatctcgaa cacaacagaa ttgcggtgga
    22441 tcgttttctt gacgaggtga ctccgccggc tgccacgcct cccaccaccc tgcacgagag
    22501 catgcgctat agtctcatgg cgggagggaa acgtgtccgg cccattttga cgattgcagc
    22561 ggcggaatcg atcgggacca ccccgcccgg cttgatggct gtggcctgtt cactggagtt
    22621 cattcatacc tattcattga ttcacgacga cctcccttcc atggacaacg acgacttccg
    22681 ccgcgggaaa ccgaccaatc acaaggtcta cggcgaagcg atggcgatcc tcgccggcga
    22741 tgcgttgctg acgatggcct ttgatctttg cagccgtccc gatttaatga agggttgcga
    22801 tgctgccagg caagtgcggt tgattcaaga gctggcctac ggctccggca acatgggaat
    22861 ggtcggcggg caggtcttcg acatccaagc cgagaacaag gacatcgatc tccccaccct
    22921 ccaaaatatt cacaagcaca agaccggtat gctgatcaga gccgctgtgc gcatgggcgc
    22981 catcgctgcc ggcgccacgg atcgccagtt ggatgatgtg acgggttatg ccgaagacat
    23041 cgggctggcg tttcaaatcg ccgacgatgt gttgaacgtc acaggcaccc gcgaggagct
    23101 gggcaagaat cccaacaccg atgcggaacg cggcaaaaag acctacccga cgttctacgg
    23161 cgtcgacggc gcaa
//
LOCUS       DATIFR010000250        19374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2094306, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000250 DATIFR010000000
VERSION     DATIFR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094306"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VLD60_14315"
     CDS             complement(<1..192)
                     /locus_tag="VLD60_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46185.1"
                     /translation="MARLIAVSIDCRNKETQQTFEEIVSRRRDYLITKGQGTGAVDML
                     LLELDEFRPQKTFDHVRELL"
     gene            complement(220..1296)
                     /locus_tag="VLD60_14320"
     CDS             complement(220..1296)
                     /locus_tag="VLD60_14320"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSB46186.1"
                     /translation="MNNTLAKHLMKPLNNSRGAAAVMVALTMVVLLAMGAAAIDIGHA
                     LVARNELQNVSDAAALAGTRALGFIYEGMTPAAQQAYVLTGGDQAIIEAAVQTAANAN
                     SAAGVAITINQSDIQIGIWDPATHTLNPTVNQPKSVRVLSRRDASANGAISTFLANVV
                     GMSSVSVGAVATADLTAVGQTAPGQLDVPFGISEYYFTQFGCGDYIKFYPNDGTPQAC
                     SAWHTFESYPANANTLRNIIDGMRTGTYQSPATQPGDDLNFINGNVASAFPNLINLYN
                     AKKDPSTGYWDVFVPVYDSPSCTPPNGPLPIIGYAEARITNVQGAPNFLISATILCNI
                     FEGNTVGGGPPYGPVLSTIPGLVE"
     gene            complement(1325..1729)
                     /locus_tag="VLD60_14325"
     CDS             complement(1325..1729)
                     /locus_tag="VLD60_14325"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSB46187.1"
                     /translation="MRKLNERGAVAAEFALLLPVLLTILFGIIEFGMIMYGREVVTNA
                     SREGARAGIVQRIPPLTAGEITTVATNYLTGTGINVADVVITVPASGGGTGTPVTVAA
                     TYNYPWLIPYIPTMLGLPTPLPIAMSTTMLHE"
     gene            complement(1752..2027)
                     /locus_tag="VLD60_14330"
     CDS             complement(1752..2027)
                     /locus_tag="VLD60_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46188.1"
                     /translation="MRTMSRILAVMTVSLVVGACAPTTISPNWGKAYQEARTKQILNP
                     TAGEQLDTVEGQDGKVSATAMEAYRKGFEKPDADFNKSVVTSGVQTK"
     gene            complement(2077..3510)
                     /locus_tag="VLD60_14335"
     CDS             complement(2077..3510)
                     /locus_tag="VLD60_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HSB46189.1"
                     /translation="MKYRDAIVAIGLVLGISGVPASYAQETAAGAPALGSMEQRDIQK
                     LELTAGKSKVLDLPVAIKRASLANPEVADTVVLSPTQIYLTGKAVGVTNLTLWNESGK
                     MMGMYDVVIAPDMSRLKENLHKALPEEKGILVTTSHDHITLSGTASSANSLSRALNIA
                     EAYAPKKVINSMQVGGVQQVMLEVRVAEMNRELIRRLGINFAFVAPDSFGISLLGQLT
                     QLSGFTQLASSTVNGIFGFNSGSTSWTGFVDALKEENLLKVLAKPNLVALNGQEAAFL
                     AGGEFPIPVPQAFGLVTIQFKKFGVGLVFTPNVLNSKHISLNVAPEVSELDFQNALRT
                     QGFTVPAISTRRAATTIELADGQSFAIGGLMRDNVKESVKKFPYLGEIPIIGALFRSS
                     SFQKNETELVIIVTPHLVKPLDMTAQTLPTDYYVEPNDFEFYWLGFSEKGYFGGKPGL
                     KSPAAEVLSNRVTRVNAMEGQVGHIVP"
     gene            complement(3569..4195)
                     /locus_tag="VLD60_14340"
     CDS             complement(3569..4195)
                     /locus_tag="VLD60_14340"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HSB46190.1"
                     /translation="MDTASQQLGSCGRWASALVISGILATPVAAMASNDGSHAVMRSP
                     EAQQVSLVVGKSTVVDMQVAIKRASLADPQIADAIVLSPKQIYVTGKGYGTTNLTLWG
                     KDDQVIAVFDLDVGIDLNRLKQHLAELLPDETNLHLKGTHDHVTISGTVSSEGRLHQV
                     LAIAEAYAPKKVLNFVKVYPEPIGSPAPDVQTVTVEVIKGTAVGSVKF"
     gene            complement(4214..5041)
                     /gene="cpaB"
                     /locus_tag="VLD60_14345"
     CDS             complement(4214..5041)
                     /gene="cpaB"
                     /locus_tag="VLD60_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSB46191.1"
                     /translation="MKRYRPLVFFGIALLLGLITSIMVFSWLQNEKNRLMAAPLPLTK
                     NVQVLVANADLSWGTKLMPEMMQAQEVPAGAIPEGHFTSVEGIKDRVLLVDIKRNELL
                     LESKLAPLGTTSGGVAAVTDVNKRAMSVKVDDVIGVAGFIKPGDRVDVMVTIEPEPNK
                     QGHTISKMILENVKVLAAGTQMERKGKDEEPKPVQVITVEVDVEEAEKLALASNQGKL
                     RLALRNPLNSERVLTKGANVGALLNSFRPKESLKRQDGEGGVRVEVIKGDVRKEVKF"
     gene            complement(5145..5666)
                     /locus_tag="VLD60_14350"
     CDS             complement(5145..5666)
                     /locus_tag="VLD60_14350"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HSB46192.1"
                     /translation="MEWTIVVLLTVLLVTAMCTDLRSSRIPNWLTFPSMGLAIVAHGW
                     SAGLPGALFSLGGLGMGLGLFLLIHLSGSIGAGDVKLMAAVGAMVGPYGALLSGLLAI
                     MVGGVYAVGAMYYHWGFFGTGRKLASAAQGALLTGGQVWAQELQLPFRLRYGLAIAGG
                     TLLFQLGLHPFGG"
     gene            complement(5870..6046)
                     /locus_tag="VLD60_14355"
     CDS             complement(5870..6046)
                     /locus_tag="VLD60_14355"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HSB46193.1"
                     /translation="MLNVIRQFVKEEEGASAAEYALLLALISLFIVGAVTLLGNNIST
                     AITAAANVIVPPAS"
     gene            6458..7462
                     /locus_tag="VLD60_14360"
     CDS             6458..7462
                     /locus_tag="VLD60_14360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB46194.1"
                     /translation="MPIQPSLRLPFIAGLLVGGSMLAGCAGSKDPASPSFDMSEYRKV
                     MEAQKGQQDSLDDPAPSAPEMTPEEHERAGDNEAQRRNFTMAGLHYSKALKADPTRNS
                     VRLKLGQIFLQQGMFEPALTQFQDLQTRDPNSAPAHQGIGHVYLQQGKLREAEAALTK
                     AIALDPSSWLAQNLLGLVYDQEQRHTEAIAAYQAALALRPREPSVLNNLGLAYALNGD
                     YDAAIKAYEQAVSAGSTSPKLYNNLGVAYAHRQRFADALESFKKATDEPRAYNNLGVA
                     LLGLGNAKVAAACFEKAIEVNPQYYEKATENLRQARQALGNSANGSSTRASSEGVSCP
                     "
     gene            complement(7775..8032)
                     /locus_tag="VLD60_14365"
     CDS             complement(7775..8032)
                     /locus_tag="VLD60_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46195.1"
                     /translation="MAWLILGYLCSHPDAKDTVEGVGTWWLNGKGIDVEAEVVRGSLD
                     YLVNLGWLMAIRRHAGVTVYALNQNRRHVLQRFMQSHSRAH"
     gene            complement(8384..9394)
                     /locus_tag="VLD60_14370"
     CDS             complement(8384..9394)
                     /locus_tag="VLD60_14370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB46196.1"
                     /translation="MSGWDKALIGTSRNFLQVTGMIPLLSTSKSTVLISGETGTGKEL
                     FARAIHYSGERRGRPFVPVNCAALPDHLIENELFGHSKGAFTGALLDKPGLLHEADGG
                     TLFLDEINSLSMVVQSKLLRVLQDQEFRPLGSTKCRTVDVKIIAATNADLRYMVEARQ
                     FREDLFYRLNVLSVALPPLRDRKEDILLLAGHFLKVYAKEFDRGAITLGHSAKTKLMG
                     YAWPGNVRELQGVLQRAVAMASGETLESQDLDLPETEKPELIGPTITLVSRGVLSNEA
                     SFQAMKSRVIEEFERAYLSELLSTHHGNISKAARAAKKERRAFQRLLQKHGLDRKIFT
                     AA"
     gene            complement(9555..10259)
                     /gene="bcsS"
                     /locus_tag="VLD60_14375"
     CDS             complement(9555..10259)
                     /gene="bcsS"
                     /locus_tag="VLD60_14375"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HSB46197.1"
                     /translation="MARTHVSCGIAFCVALLWPGAVAAGDVFTGYQIDNEGEYYTYLG
                     IRTPISSGESNLQPFVQVMGAGLGYTFKDNGAKRDAEVQFATPSLGLKYVLDQWNLIG
                     FVGPQFRWKQEDLPGGGRSSKQDVGVYVQGEAFYWHEQGTFHAIASYTDLDSFVWSRL
                     RGTRLVHKSEEGCCSVYLGGDLAGMGNDQFYAVQAGPVLQVPIDRFYFTVKGGYQYTQ
                     TFRSGAYGGLELYFPF"
     gene            complement(10229..12499)
                     /locus_tag="VLD60_14380"
     CDS             complement(10229..12499)
                     /locus_tag="VLD60_14380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB46198.1"
                     /translation="MTWLNQFYSSSVAIPLSLVALAVISLLSWRKRGHQVILALTLFL
                     YARYLLWRGLYTLNNGDWPSFLVSWTVYTAEAYAFVQILLFAYHAWSPLERQPVPLRR
                     YPIVDIFVTVVDEPVAILRRTLVGCLNQDYPKDRYRVYVLDDGRRDELKSLASSLNCG
                     YIRREDRQHAKAGNLNHALQYTSGEIIAIFDVDHVPTDRFLRDTVGFFQDDLVAFVQT
                     PHHFYNPDVFQKNLQLEGVLKNEQALFYRVLQAGRDRHNSAFFAGSCGLFRRRMLMEI
                     GGFRTETVTEDIHTSMAIHAQGYRSCYLNKVLAAGLMPETFESSMKQRIRWAMGHVQI
                     FFQTNPFTMRGLTLGQRFGYFASIFYFLHGVPRVVCLIAPLFALLLGIIPVAADVPSL
                     VNFFGSYYLATLVMLRTVSRGTRNAFWSDIYETAGSVALSVATLKTALRPRKKRPFVI
                     TPKGVGQERRGFSRFSYVVPHLIIMGLLIAGLVTGIRLWMEDVPLPGLEVSVFWASVN
                     LVLIGVAILAAAELPEWRRIFRIRRQMTCELVAGEERLSGTVEDINETGVLVRASRPV
                     LGGNDQLYLSVNSPMGGHLTVGARICRQERVSSDIVEIGLKFVDVDDKTSDSFIAATF
                     SDPRAWNQPEVEPGIFRSLWSILRVFRMIFAKSRTSHRRYLRIPFRQDCRLVFEGRVL
                     NGTIGRISKTGLSVNIPGTVDLLGEDGTLYVESFALKVRRRWAMQCEGEVVAGFAIEQ
                     VNKGADQWQELTSLAA"
     gene            13029..14060
                     /locus_tag="VLD60_14385"
     CDS             13029..14060
                     /locus_tag="VLD60_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB46199.1"
                     /translation="MTTPRIVIALVFFIVLAGLFHSSFQFQSSAPEPAISSVVATVNG
                     RSITLREIEQAAALPLYQIDQQRSQLLQQALQRKIEETLLEAEASRAGVSVSQLLTEA
                     SQSDYIARLANLPAPVKRLSPGTTVDDQAHAASHDPQEQARIRQALLVSLRRKADIRI
                     ALPPQEPPILSVSVDDDPSIGPENAPITIVEFSDFQCPYCQKSVGILKELRRLYGEKI
                     RLVYRDYPGPNHPHATQAAEAAQCAAEQGKFWEYHDLLFDRQTPGKGWDFPALAKELG
                     LQQDTFTACLITGRYREEVIKDFYDGMKLGMTSTPTFFINGRPVVGAQPLTTFQALID
                     KLLDPQPRS"
     gene            14124..14768
                     /locus_tag="VLD60_14390"
     CDS             14124..14768
                     /locus_tag="VLD60_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HSB46200.1"
                     /translation="MDEQSRLTKTKEQWAKARRGGEEREVFEQGDDRLPPGQHLVETF
                     PVLDLGFKPEVSLAEWKLTIGGFVANPITWTWEQFLAQPQFKDVSDFHCVTSWSRFDN
                     EWEGVSFKHIISVAKPLSLAKFVLFKSYDDYTTNLPLEACDDQDVLLTYKWNGKPLTK
                     EHGGPVRVIVPKRYAWKGAKWVKEITFADKDEKGFWEVRGYSNTALPFLNDRYA"
     gene            14900..16006
                     /locus_tag="VLD60_14395"
     CDS             14900..16006
                     /locus_tag="VLD60_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HSB46201.1"
                     /translation="MASPESFIHTIEFWPVDIPLTDPFVVATGSRVVAENVFVRITLS
                     DGKQGYGEAAPFPEVGGESRASCLAALAQLGRTLLGSSVDQFTAAAKHMQDVAPGQPA
                     ARCALETALIDAHCHARQLPMWKLWGGADVRKRETDITIPICDREKTLTLVRGWYAKG
                     FRLFKMKVGSDVDEDIRRLEAVHRALLGISFIGDGNQGFSRNECLAFAKGVTRFGGNM
                     LLLEQPLVREDLEGLAAIRRDTGIPVAADESVRSIADAQQVVECGAADYINIKIMKTG
                     VVEARDIAAFTLASGLKLMVGGMVETRIAMGCSFSLVLGMSGFEILDLDTPLLLGFDP
                     ISGGYRYEGPRLIPWNEPGLGMAVSPSTESIVLT"
     gene            complement(16152..16343)
                     /locus_tag="VLD60_14400"
     CDS             complement(16152..16343)
                     /locus_tag="VLD60_14400"
                     /inference="COORDINATES: protein motif:HMM:NF023510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HSB46202.1"
                     /translation="MEQKIVGYHQDEQGDWVADLACGHGQHVRHQPPMTSRPWVLTDA
                     GRQKHVGEDLNCKKCDEDS"
     gene            complement(16407..16862)
                     /locus_tag="VLD60_14405"
     CDS             complement(16407..16862)
                     /locus_tag="VLD60_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46203.1"
                     /translation="MSRWFRGQLLVVLISAAAACNSDVPVSATTDIVPPGYHVAVTRV
                     ATHPFLARYNLKLTVTTTSGCSAASELFPDTGGVSRRNLYLTDSGRLFVVGQFDVRRF
                     DEQSCRVELIEFRSLEAGMSFQGSFDVDSSGTWTFFPVGVRAERLFDRL"
     gene            complement(16859..17401)
                     /locus_tag="VLD60_14410"
     CDS             complement(16859..17401)
                     /locus_tag="VLD60_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB46204.1"
                     /translation="MTLRGQLLGCFLAGIATLSLAEGRIATMVYAADELPKESVLPLS
                     MAGKAVQAALDVCKKDGYRVSVSVVDRAGVLRTMGRSDGAGPHTVESSRKKAYTAASL
                     RRPTTELAELIMKVPTLQSLRDMNENILILGGGLPIEIGGEVVGAIGVGGAPGSHLDD
                     ACAQAGLDAIGAAPKVSAAK"
     gene            complement(17530..17715)
                     /locus_tag="VLD60_14415"
     CDS             complement(17530..17715)
                     /locus_tag="VLD60_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46205.1"
                     /translation="MASYRQELERRGPDGIPSLDDVEPGKICGAIIPMSERAQTQMRD
                     SIEVIDYLLNQERVVWS"
     gene            complement(17869..18894)
                     /locus_tag="VLD60_14420"
     CDS             complement(17869..18894)
                     /locus_tag="VLD60_14420"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSB46206.1"
                     /translation="MRMTLYMALVLGIIPLASGVGRAETPVVQAPRTITVALDGSGDF
                     TSIQEAVDTAKKGDTVFLKPGRYQQDVTVHSKDGVKLVGAGQDKVTLLGREDVVGALH
                     VGKWPYGATNIEISDMTINEHGGHAVGLFNGRGVLLRRLTINGMVFSQQVEDARIEDC
                     TIGGSETTGVQFADSQAVLIGNFIHDNDHGVNVAGKSNVRLERNIITRSLFEAVVVND
                     RAKAVLVSNTLVKNGGGAAFLGQSQSEASGNVVGLNRVGFLVASSSRASTSFNAFYNT
                     DGNYMRPGSPNQPAPELQAQSDITGDPFFVDPGHDDFRLRGDTPLVKVGAFPFLGALP
                     PAAQPTR"
     gene            18962..>19374
                     /gene="coaE"
                     /locus_tag="VLD60_14425"
     CDS             18962..>19374
                     /gene="coaE"
                     /locus_tag="VLD60_14425"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSB46207.1"
                     /translation="MLFSPAMILVGLTGGVATGKSTVAKMFKQCGAVVIDADEIARTV
                     VKPGKPAWRQIVKSFGRSVLAPDRTINRHALGAIVFRHPAKRRLLERIVHPRVAREQA
                     KRTKQAARHDPHAVVIYDVPLLFEVGIDKRVDRTIV"
BASE COUNT         4226 a         5770 c         5147 g         4231 t
ORIGIN      
        1 taatagttcg cgaacgtgat cgaacgtctt ctgaggacgg aattcgtcca gctcaagcaa
       61 caacatatcg accgcgccgg tcccctggcc tttagtgata agatagtcgc gccgccgtga
      121 aacgatttct tcaaaggtct gttgtgtttc tttgttccga cagtcgatcg acactgcaat
      181 caatcgagcc ataccagcat atctcctcgg ttgttcgaat tattcgacaa ggccaggaat
      241 cgttgaaagc accggcccat atgggggtcc acctccaact gtattccctt cgaaaatatt
      301 gcacaggatc gtcgccgaga tcagaaaatt cggcgcgcct tggacattcg tgatcctggc
      361 ttcggcgtac cctatgatcg gaaggggccc gttgggcgga gtgcagcttg gagaatcgta
      421 cacgggcacg aagacgtccc aatagccggt gctcgggtct tttttggcat tgtagagatt
      481 gatcagattc ggaaaagcgc tggccacgtt gccgttgata aaattcagat cgtctcccgg
      541 ctgcgttgca ggcgattggt aggtgccggt acgcataccg tcaatgatat tcctcaatgt
      601 gttcgcattg gccgggtaac tctcgaatgt atgccaggcg gaacaggcct gcggcgttcc
      661 gtcattcgga taaaacttaa tgtagtctcc gcagccgaac tgggtaaaat aatactccga
      721 aataccgaag ggcacgtcca actgacccgg cgccgtttga ccgaccgccg tcaggtctgc
      781 cgtcgctacg gcaccgacgc tgacgctcga catcccgacc acattcgcaa gaaacgtact
      841 gatcgccccg ttggccgaag catcccgtct cgacaacact cgtaccgatt tcggttgatt
      901 gaccgtggga ttcaacgtgt gagtcgccgg atcccagatc ccgatttgaa tgtccgactg
      961 attaatggta atcgcgaccc ctgccgccga gttcgcattg gccgccgttt gcacggcggc
     1021 ttcgatgatt gcctgatcgc cgcccgtgag gacgtaggcc tgttgcgcag ccggagtcat
     1081 gccctcgtag atgaatccga gagcacgcgt gccggccaag gccgcggcat cggacacatt
     1141 ttgcagttcg ttcctcgcca ccaaggcatg accgatgtcg atcgcggcag cacccatcgc
     1201 cagtaagacc accatcgtga gcgccaccat cacggcagcc gcgccacggg agttgttcag
     1261 tggcttcatg agatgttttg ctaatgtgtt gttcatctta tggcgcctcg tccggttcat
     1321 cgcgttactc atggagcatg gtggttgaca tggcaatcgg aagaggcgtg ggtaatccca
     1381 acatggtcgg gatataaggg atcagccaag gatagttata agtagcggcc acggtcacag
     1441 gggtgccggt tcctcctcct gaggccggca cggtgatcac gacatccgct acgttaatgc
     1501 ctgtgccagt cagatagttt gttgccacag tcgtaatctc tcccgctgtg agtggaggaa
     1561 tccgttggac aatgccggcc cgcgcccctt cccggcttgc attggtgacg acttcgcggc
     1621 cgtacatgat catgccgaac tctatgatgc cgaagaggat ggttaagagc accggcaaca
     1681 aaagagcaaa ctctgctgcc acggcgcccc gctcgttcag ctttcgcatc ggatccgtcc
     1741 cctccatcca gctacttggt ttgcacaccg ctggtcacga ccgacttgtt gaaatccgca
     1801 tccggcttct cgaatccttt acgataggcc tccatcgcgg tggcactcac tttcccgtcc
     1861 tggccttcga ccgtgtcgag ttgttcaccg gccgtgggat tcagtatctg tttcgtccgc
     1921 gcctcctgat aggctttccc ccagttcggg ctgatcgtcg taggagcaca ggctccgact
     1981 accagactta ccgtcatcac tgccaagatc ctgctcattg ttctcattgc aatctccttc
     2041 tcatatctga ctcggtccaa tttgaagctg tctcgcttac ggaacgatgt gcccaacctg
     2101 tccttccatc gcgttgactc tcgtcacacg attagaaagc acttcggccg cgggcgactt
     2161 taggcctggc tttccgccga aatatccctt ctccgaaaac cccagccagt agaactcgaa
     2221 gtcgttgggc tctacatagt agtccgtcgg taacgtctgc gccgtcatat ccagcggctt
     2281 caccaaatga ggcgtgacga tgatcaccaa ctccgtttcg ttcttctgga acgagctgct
     2341 ccggaacagg gcgccgataa tcgggatctc gccaaggtac ggaaattttt tcaccgactc
     2401 tttgacgttg tcgcgcatca atccgccgat cgcaaaactc tggccatcgg ccagctcgat
     2461 cgtagttgca gcgcgtcttg tcgaaatggc cggcacggtg aacccctgag tccgcagggc
     2521 gttctggaag tccaactccg acacttcagg cgccacgttg agactgatgt gtttgctatt
     2581 cagaacgttc ggggtgaaga ccaagccgac cccgaacttc ttgaactgga tcgtgaccaa
     2641 tccaaaggcc tgaggcacag gaatgggaaa ctcacccccg gcgagaaatg ccgcttcctg
     2701 cccgttcaag gccaccaagt tcggtttggc gagtaccttg agcaggtttt cttccttgag
     2761 cgcatccaca aatcctgtcc acgacgtcga ccctgagttg aacccaaaga ttccattgac
     2821 ggtcgaggac gcaagttggg tgaagccgga aagctgggtg agctgaccaa ggaggctgat
     2881 cccgaagcta tcaggtgcca caaacgcaaa gttgatccct aagcggcgga tcagctctcg
     2941 attcatctcc gccacgcgta cttcgagcat gacctgttgc acgccaccga cttgcataga
     3001 attgatgact ttcttgggcg cataggcttc ggcaatgttc agggcgcggg aaagactgtt
     3061 ggcgctcgag gccgttcccg acaaggtaat atgatcgtga ctggtggtca caaggatgcc
     3121 tttttcctcc ggcaacgctt tgtggagatt ctccttcagg cggctcatgt cgggtgcaat
     3181 cacgacgtca tacatgccca tcatcttccc gctctcgttc caaagcgtca gattggtgac
     3241 cccgaccgcc ttgccggtga ggtaaatctg tgtcggggac aacaccaccg tatccgcaac
     3301 ttcgggattg gccaaggagg ctcgcttgat ggccaccggc aaatccagca ctttcgactt
     3361 gcccgcggtg agttccagct tctggatgtc gcgctgttcc atcgagccga gagccggtgc
     3421 gccggctgcc gtttcctgtg cataggatgc cggaaccccg gagatcccga gtaccagccc
     3481 aatcgccacg atcgcatcac ggtatttcat tgacgtgctc cttcaacatt tgttgccaat
     3541 attgttccgc tcgtccaatc caacggcctc agaacttcac ggaaccgacg gctgttcctt
     3601 tgatcacttc tacggtcacg gtctgcacat caggtgcggg acttccgatc ggctcaggat
     3661 aaaccttgac aaagttcaac actttcttcg gcgcataggc ctccgcaatg gccaggacct
     3721 gatgcaaccg cccttcgctc gagacagtcc ccgaaatcgt cacatgatcg tgggttcctt
     3781 tgagatgaag attcgtttcg tccggcaaca actctgcgag atgctgcttc aaccggttga
     3841 gatcaatccc cacatcgagg tcgaacacgg cgatcacctg gtcatccttt ccccagagcg
     3901 tgagattcgt cgtgccatag cctttgcccg taacgtagat ctgcttcggg ctgaggacga
     3961 tggcgtccgc gatctgcgga tcggccagcg aggcccgctt gatggccacc tgcatatcca
     4021 ccaccgtgga cttgcccacc accaggctta cctgctgtgc ttccggcgat ctcatgacag
     4081 catggctgcc gtcgttcgac gccatcgctg cgacaggcgt ggccaagatc ccgctgatga
     4141 ccagcgcact cgcccatcga ccacaagacc cgagttgctg actcgctgta tccatatcgt
     4201 gcctccgtaa atcctagaac ttgacttcct tgcggacgtc gcctttgatg acctcaacac
     4261 gaactccgcc ttccccgtct tgccgcttga gcgactcctt cggacggaac gaattcaaga
     4321 gcgctcctac attggccccc ttggtcaaga cccgctcgct attgagcgga ttccgcagcg
     4381 ccaacctgag tttgccctgg ttcgaggcca aggccagctt ttccgcttcc tccacgtcca
     4441 cttccacggt aatcacctgg accggtttcg gctcttcgtc cttgcctttt cgctccatct
     4501 gcgtgccggc cgccaggacc ttcacgttct ccagaatcat cttggagatc gtatgcccct
     4561 gtttgttggg ctccggctca atggtcacca tcacgtccac gcgatcgccc ggcttgatga
     4621 accctgccac gccgatgacg tcatcgactt tgaccgacat ggcacgcttg ttgacatccg
     4681 tcacggccgc cactccgcca ctcgtcgttc cgagtggagc taacttcgat tccagaagca
     4741 gttcgttcct cttgatgtcg accaagagca cccgatcttt gatcccctcc acgctcgtga
     4801 agtggccttc gggaatcgct ccggcaggga cctcctgcgc ctgcatcatt tccggcatca
     4861 gcttggtccc ccaggaaagg tccgcattcg ccaccaagac ctgcacgttc ttggtcaagg
     4921 ggagcggagc cgccatcaac cggttctttt cgttctgcaa ccaggagaac accatgatgc
     4981 tcgtgatcaa cccgagcaac agcgcgatcc cgaaaaatac aaggggccga taccgcttca
     5041 ttgtttcgtc tcctcttctg tcttggcgat ctgcctcatc tctgattgcc tcgtcatctg
     5101 tctcaatccc gtctgtttca gtctagccgg gttccgtcag atgcctatcc cccgaaggga
     5161 tggagtccca gttgaaaaag tagggtaccg cccgcaatag cgagcccata gcgcaggcgg
     5221 aacgggagtt gtaattcctg agcccagacc tgtcctcccg taaggagggc tccttgggca
     5281 gcagaggcta gctttcgacc ggtgccgaag aagccccagt ggtagtacat cgcgccaacc
     5341 gcataaacgc ccccgaccat gatcgcgagt aatccggata agagtgcgcc gtaggggcca
     5401 accattgcgc cgacggccgc catcaatttc acatcgcccg ctccgatgct cccggacaaa
     5461 tgaatcaata agaagagccc taaacccatg ccaagaccgc ctaagctgaa cagcgccccc
     5521 ggaagcccgg ccgaccagcc atgggcgacg attgccaaac ccatcgaggg aaaagtcagc
     5581 caatttggaa ttcgagatga acggaggtcg gtacacattg ccgtgacgag caggaccgtc
     5641 aacagcacca cgattgtcca ttccatcacc gatcctcctc tatgctgact gtccgacgaa
     5701 tccgcatcat catatttaat tctcgattct tcattcctgc ttccgaagcc cttgcagcta
     5761 tcgcctttta cttcgaaatt cttttccgct actgttcact tcggcggaag aaacctgctg
     5821 gcccgcttct gacaggccag cagtttctcc gtggtcaaga tgacctggct tagctagccg
     5881 gaggaacaat cacgttcgca gcggccgtaa tcgccgtgct gatgttgttg ccgagaagcg
     5941 tcaccgcacc gacgatgaac agggagatca acgccagcaa caaggcatac tcagcagccg
     6001 aggcaccttc ttcttccttc acgaactgac gaatgacgtt caacatgtta ttcacctttc
     6061 tattgtgtaa agttatggtt tctgtcactc gaatcatttt catatacgca acgagacttt
     6121 cagtccgcga ccgaatcaac caatcgggaa ttccctcacc tccttgaccc gggttgtcgc
     6181 gtctaacagt cccttacgca ttgctccaaa gccattgcaa agcagcggcc aattgccacg
     6241 attctctaac cagctgttaa tcttgaccga ttcaattccc gcctctcctg gctgcggcat
     6301 gcgaacctca gagaacaacc cgcacttcct tctcctgctg cggcgcagga gccgcacatc
     6361 cgtatcaagg aaagcgtttt gttttgacca tctgctgggg ttgacgtaat catgaaccat
     6421 cagaacctgg ctcccaacgc gatagatgag gagatgtatg cccattcagc cctctctccg
     6481 attaccgttc attgcaggcc tgcttgtggg cggatcgatg ctcgccggat gtgccggctc
     6541 gaaagacccc gcctctccgt cgttcgacat gtcggaatat cgaaaggtga tggaagcaca
     6601 gaaggggcag caggacagcc tcgatgaccc tgctccatcg gctcccgaga tgacgccgga
     6661 agaacacgag cgcgcaggag ataacgaggc gcaacgccgc aatttcacga tggcaggcct
     6721 ccactacagc aaggcgctga aagcggatcc cacccgaaac agcgttcgcc tcaagctggg
     6781 ccagattttt ctgcagcagg gaatgttcga gccagcgctg acacagttcc aggacctcca
     6841 aacgcgagat cccaattcag cccctgccca tcaagggatc ggtcatgtct acctgcaaca
     6901 aggcaagttg cgagaggctg aggcggccct caccaaggcc atcgcactcg atccgtcgag
     6961 ctggcttgct cagaacctgt tggggctcgt ctatgaccag gaacaacgcc ataccgaggc
     7021 gatcgcagcc tatcaagcgg ccctagcgct ccgtccacgc gaaccaagcg tgttgaacaa
     7081 ccttggcctc gcttatgcgc tgaacggaga ttacgacgca gcgatcaagg cctatgagca
     7141 agcggtctcg gccggctcga cctctcccaa gctctacaac aacctaggtg tggcctacgc
     7201 ccaccggcag cgatttgccg atgcgctgga atcgtttaag aaagccacgg atgaacctcg
     7261 agcctataac aacctcgggg tggccttact cggtctgggg aatgccaaag tggccgccgc
     7321 ttgcttcgag aaggcgatcg aggtgaatcc gcagtattat gaaaaggcca cggagaatct
     7381 taggcaagca cggcaggcgc ttgggaattc cgcgaatggc agctccacgc gtgcctcgag
     7441 cgaaggcgtc tcgtgcccat gatctcagca ggatgttgaa aaagttcgcc gctgcgttct
     7501 cgcgtcgctc agaggctcaa cgtacgaaga atatctattg aggtacctct ccgctcgcgt
     7561 gtgatcgaag cgagcggtca agcacagctt ggtatgtacc tcttcgcctc gtcgctcgct
     7621 gcggccttgc ttggtgaaag gcgcgtctcg cgcgccgggg atgggcgggt gagaacggtg
     7681 gcctttttga acatcctgtg gcctaccctg ttgccatccg tgacgggtca cgagcgtcac
     7741 tttccgggat gctcaaaacg tttttaacaa cctgttagtg cgccctcgaa tggctttgca
     7801 tgaaccgctg tagcacgtgc cgacggttct ggttcagggc gtacacggtg acgcctgcat
     7861 ggcgtcttat tgccatcagc catcccaagt tcaccagata gtcaagcgag ccccgaacga
     7921 cctctgcctc gacgtcgatc cccttgccgt tcagccacca agttcccacg ccctccaccg
     7981 tatccttcgc atcgggatgg gaacagagat agccgaggat cagccatgcc acgacatcat
     8041 tttcctcgga gaagtccggc ctgctcatgg ccgacataac agagcaattt cagtaccggc
     8101 gtgccggcct tctaatatgc gtgattttta atgaatcacg ttctctccca cgcttctcct
     8161 gcggctcgct ctcctcagga taggctgccc tagcccgcac tattcatggc ggttcgtcgc
     8221 gaaattgcgg agtcgcgaag cgagacgggc gcgtggagcc acgcggaagg gaggcatact
     8281 tgaaacagta tgtcgaccgg ccaagtggcg agcccgcccg ccgataggct tgtcacagcc
     8341 gcgaatccgc gattgcagca gaagcgttca tgaataatgc gggctaagct gcggtaaata
     8401 tctttcgatc cagcccatgc ttctgcagga gacgctggaa cgcgcggcgt tccttttttg
     8461 cggcacgtgc ggccttggag atgttgccgt gatgggtgga cagtagctcg ctgagatatg
     8521 cccgctcgaa ttcctcaatc actctcgatt tcatggcctg gaaactcgcc tcgttggata
     8581 gtactcctcg cgagacgagc gttatggtcg gcccaatcag ctccggcttc tcggtttccg
     8641 gcagatcgag atcctggctt tcaagcgttt ctcctgatgc catcgctaca gcgcgctgca
     8701 agacaccctg cagctctctc acgttgccag gccaggcata gcccatcagc ttcgtcttgg
     8761 ctgaatgccc gagggtgatg gcgcccctgt cgaactcctt ggcgtagacc ttcagaaagt
     8821 gaccggcgag aaggaggatg tcctctttcc ggtcacgcaa gggtggcagc gcgacggaca
     8881 agacattcaa gcggtagaac aggtcttcgc gaaattggcg agcttcgacc atatagcgca
     8941 agtcggcgtt ggttgcggcg ataatcttca catcgacggt tcgacacttg gtcgagccga
     9001 gcggcctgaa ctcctgatcc tgcaggacgc gcaggagctt actctgcacg accatgctga
     9061 gcgaattgat ttcgtcgaga aacaacgttc cgccgtcggc ttcatgcagc aggcctggct
     9121 tgtcgagcaa tgcgcccgtg aaggctcctt tgctgtggcc aaacaactca ttttcgatca
     9181 aatggtcggg caatgccgcg cagtttacgg gaacgaaagg cctcccccgc cgctcgccgg
     9241 agtaatggat ggctcgcgca aatagttcct tgcccgtccc cgtttctccg gatatcaaca
     9301 ccgttgactt ggacgtgctc aatagcggaa tcatccctgt cacttggaga aagtttctgc
     9361 tcgtcccgat caacgccttg tcccaaccag acatgtataa ccctcctcga attacggacg
     9421 accgattcgg atttctccgt tctccgtatg gcgaccctcg gctgcctccc ctacgcactg
     9481 ctcacaaatt cttccgctcc ccttgcgttg tccgacattc ccgttctccg gctatggagg
     9541 cccctatccg gagcctaaaa cgggaaatac agttcgagtc cgccgtacgc cccactccga
     9601 aatgtttgcg tgtattggta cccgcctttc acggtgaaat aaaaccggtc aatcggcacc
     9661 tgaaggaccg gtcctgcttg gaccgcatag aactggtcgt ttcccattcc tgcgagatcc
     9721 ccaccgagat agacggagca acaaccctcc tcgcttttat gaaccagtct ggtgcctctg
     9781 agacggctcc acacaaaact gtccaagtct gtataggacg cgatggcatg aaaggttccc
     9841 tgctcgtgcc agtaaaacgc ttctccctga acatacacgc cgacgtcttg cttgctgctt
     9901 cttcctcccc cggggagatc ctcctgtttc caccgaaact gaggaccgac gaatccgatg
     9961 agattccatt ggtcgagaac atacttcagc ccgagagatg gggtcgcgaa ctgaacctca
    10021 gcatcacgct tcgctccgtt gtctttgaat gtgtaaccga ggccggcacc catgacttga
    10081 acaaagggtt ggagatttga ctctcccgag ctgatcggcg ttcggatacc caaataggtg
    10141 tagtactctc cttcattgtc gatttgataa cccgtaaaga catccccggc tgccacggca
    10201 ccgggccaca acagggctac acagaatgct atgccgcaag agacgtgagt tcttgccatt
    10261 ggtctgctcc cttgttcacc tgttcgatcg cgaagcccgc cacgacctcc ccctcacatt
    10321 gcatcgccca tctccggcgg acctttagcg caaaggattc cacatacaga gtgccatcct
    10381 cgccgagaag atcgaccgtt ccaggaatgt tcacggaaag gccggttttt gaaattctgc
    10441 cgatggttcc gttcagaaca cggccttcaa ataccagccg gcagtcttgt cgaaagggga
    10501 tacggagata gcgccgatgc gacgtcctcg acttggcaaa aatcattcga aacacgcgga
    10561 ggatggacca taagctccgg aatatcccgg gctcgacctc gggttgattc caggcgcgcg
    10621 gatcggaaaa cgttgcggca ataaatgaat cggaggtttt atcatcaacg tcgacgaact
    10681 tcagtccgat ctcgacaata tctgatgaca ctcgttcctg acggcagatt cgcgctccga
    10741 ctgtaagatg accgcccatc gggctgttga cgctgaggta gagttggtca tttcctccca
    10801 acactggcct gctcgcccga acaagcacgc cggtttcatt gatgtcttcc accgtgccgc
    10861 tgagtcgttc ctctcccgca accaattcgc atgtcatctg acgcctgatg cgaaatattc
    10921 tccgccactc cggcagctca gcggcagcca aaatagccac accgatgaga acgagattga
    10981 ccgaagccca gaacacactc acctctaatc cgggaagcgg gacgtcctcc atccacagcc
    11041 gtattccggt cactaatccg gcgatcaaca gtcccatgat gatgaggtga ggcacgacgt
    11101 acgagaaacg tgagaagccc cgccgctctt gtcctactcc tttgggcgtg atgacgaacg
    11161 gccgtttctt cctgggcctg agggcggtct tgagggtggc cacactcaag gcgacgctgc
    11221 cagccgtctc gtaaatgtcg gaccagaagg cattgcgtgt accccggctg acggtcctca
    11281 acattacgag cgtcgccaaa taataggaac cgaaaaagtt cacgagcgac gggacatcgg
    11341 ctgcaacggg aataattccc agtagaagcg caaacagcgg agcgatgagg cacacgacgc
    11401 gaggaactcc atggagaaag tagaaaatgg aggcaaagta tccgaatcgc tggcccagcg
    11461 tgaggccgcg catcgtgaac ggattcgttt gaaagaaaat ctgcacgtgg cccatggccc
    11521 accggatacg ctgcttcatg gaactctcga acgtctccgg catcagtccg gcggccagta
    11581 ctttgttcag gtagcatgac cgatagccct gcgcatggat ggccatgctt gtgtgaatgt
    11641 cttcagtcac ggtttcggtg cgaaacccgc caatttccat caacatgcgc cgacggaaga
    11701 gcccgcagct gcccgcaaag aatgcgctgt tatggcggtc gcggccggct tgaaggacac
    11761 gatagaacaa ggcctgctcg ttcttgagga ctccctcgag ctgcaggttc ttctgaaaca
    11821 cgtccgggtt gtagaaatga tgcggcgttt gaacaaaggc gaccagatca tcctgaaaaa
    11881 atcccaccgt gtctcttaag aaacgatcgg tcggaacgtg gtcgacgtcg aagatcgcga
    11941 taatttcacc cgacgtgtat tggagggcat ggttgagatt cccggctttc gcgtgttgcc
    12001 ggtcttccct gcggatgtac ccgcagttca acgacgaggc taagctcttc agttcgtctc
    12061 gcctgccgtc gtccagcaca tacacccgat agcgatcttt aggatagtcc tgattgagac
    12121 agccgacaag cgtacgacga agaatagcca ccggttcatc gaccaccgtg acgaaaatat
    12181 cgacaatcgg atagcgacgc agcggaaccg gttggcgttc aagcggtgac catgcgtggt
    12241 aggcaaataa caggatctgt acaaatgcgt atgcttccgc cgtataaact gtccaactca
    12301 cgaggaagct cggccagtcg ccgttgttca gagtatagag gcctcgccac agcaggtatc
    12361 gtgcgtacag gaacagggtg agggccaaaa tgacttgatg cccccgtttc ctccagctga
    12421 gcaggctgat cacagccagg gccacaagtg acaaaggtat ggctacggat gagctgtaaa
    12481 actgattgag ccacgtcata ggcgtttcct ttgtgagtct cccggtgaat cctgccggtc
    12541 agctgccggc ctcgaccccg ctttctgcgg ctgtttcccc cctgcggcgt acataactca
    12601 gcctagaatg gaaaggccct ttcgcctata ccgaggatgg aggggaagcc ggcatacagg
    12661 agaggtactt ctttggaggg gcaataatgc cgcacccggt ggatgaagtc ggctgacaac
    12721 cacaggccgg ggattcaagt taggagtctg cccgagccat cccttcgtcg cgaggcgaag
    12781 gaggtgtggg gagctgctag gccgcagccc cgaaaaaccc ggaagcgtat tcgctgaaat
    12841 acgttgagga tttttcgggc cgagaacgac gcagatgacc acgaatcgtt tgccgcagca
    12901 gaatggtgat gactcggaca gactccgagt gcggattatc aagaaatcat ggattttctg
    12961 tgacctcgtc gctatggttt cagacagcgg tccacaccag gaattccaca gttttcagtc
    13021 agacagcaat gacgactccc cgcattgtaa ttgccctcgt cttcttcatc gttctggctg
    13081 gtctttttca tagctctttt caatttcagt cctcagcgcc tgagccggcc atctcctcgg
    13141 tggtggcaac cgtgaacggc cgctccatta ccttgcgaga aattgagcag gctgcggccc
    13201 ttccccttta ccaaattgac cagcagcgga gccaactact ccagcaagcg ctccaacgga
    13261 aaatcgaaga gacgctgctg gaagcggagg catctcgagc aggcgtaagc gtctcacagc
    13321 ttctgactga ggcttctcaa tcagattaca tcgctcggct cgcgaacctc ccggctccgg
    13381 tcaaacggtt gagcccgggc acgactgtgg acgaccaggc ccatgccgca tcgcatgatc
    13441 ctcaggagca ggccagaatc cggcaggcct tgctggtctc tctgcgccgc aaagccgata
    13501 ttcgcattgc ccttccgccc caagagccgc cgatcctctc cgtcagcgtt gacgacgatc
    13561 cctcgatcgg accggagaat gcgccaatca ccatcgtgga attttcagat tttcaatgtc
    13621 cctactgcca gaagagtgtg ggaattttga aagaacttcg ccgtctttat ggtgagaaaa
    13681 ttcgattggt ctatcgggat tatcccggcc ccaaccatcc ccatgcgacg caagcggccg
    13741 aagccgctca atgcgccgcc gagcagggca agttttggga gtaccacgac ttgttgtttg
    13801 accggcaaac cccaggaaaa ggatgggact ttcctgcact ggcgaaggaa ctgggccttc
    13861 agcaggacac cttcaccgcc tgcttaataa caggacgtta tcgcgaggaa gtgatcaaag
    13921 acttttatga tggcatgaaa ctcggcatga ccagcacgcc gacgttcttt atcaacggtc
    13981 gtccagtggt cggtgcacaa cctctaacta cctttcaagc cttaatagac aaactgctcg
    14041 atccccagcc ccgttcataa cgttcatctt gtggcttgta agatctcacc gtcctatcct
    14101 actctgtacg gggaggccga tagatggatg agcaaagccg attgaccaag accaaagagc
    14161 agtgggcgaa agcgcggcga gggggtgaag agcgggaggt cttcgagcag ggtgatgacc
    14221 ggctcccgcc ggggcagcat ctggtcgaaa cttttccggt actggatttg gggttcaagc
    14281 cggaggtctc gctggccgaa tggaagttga ccatcggcgg attcgtcgcc aatccgatca
    14341 cctggacatg ggaacagttc ttggcccaac cgcaattcaa ggatgtatca gactttcact
    14401 gtgtcaccag ttggagtcgc ttcgacaacg aatgggaagg cgtgagcttc aagcacatta
    14461 tttccgttgc gaaaccgctt tcccttgcca agttcgtcct tttcaaatct tacgacgact
    14521 atacgacgaa cctgccgctc gaagcctgcg acgatcagga tgtgctcctc acctacaaat
    14581 ggaatggcaa gccgctcacg aaagagcacg ggggtcccgt tcgcgtgatc gttccgaaac
    14641 gttatgcatg gaaaggggca aagtgggtga aggaaatcac gttcgcggac aaggatgaga
    14701 aagggttctg ggaagtccgg ggttattcga acacggcgct gccgtttctc aacgatcgct
    14761 acgcttaact gggcctgaaa taagccttct actgcatccc tctttcaatc agacactcca
    14821 tcggcggccc cttcttaacg gtctgagaga cagccatacg ttggcatcac cgcagtttct
    14881 cgttaagatg ccgcctgcga tggcgtctcc agaatcattc attcacacaa tcgaattctg
    14941 gccggttgac attcccctta ccgatccgtt cgtcgtcgcc acgggatctc gagtcgtcgc
    15001 ggagaatgtc ttcgtcagaa tcacgctcag cgacggtaaa caagggtatg gagaagcggc
    15061 gccctttccg gaggtcggcg gagaaagccg agcttcttgc ctggcggccc tcgcccagct
    15121 tggtcggacc ttgctgggct cgtcagtcga ccaattcacg gctgcagcaa agcacatgca
    15181 ggacgtggca ccggggcagc cggcagcgcg ttgcgccttg gaaacggcgt tgatcgacgc
    15241 ccattgccat gcacggcaac tcccgatgtg gaaactctgg ggtggagcgg atgtgcgtaa
    15301 gcgcgaaact gacattacca tccctatttg cgaccgcgaa aagacgttga ccctggtgcg
    15361 agggtggtac gccaaagggt ttcgtctctt caagatgaaa gtcggcagcg acgtcgacga
    15421 agacattcgg cggcttgaag ccgttcatcg ggcgttgctc ggcatctcct tcatcggcga
    15481 tggcaatcaa ggattctccc gtaacgagtg tctcgccttt gccaagggcg tcactcgatt
    15541 tggaggaaat atgttgctgc ttgaacaacc gcttgtccgg gaggatctgg aaggacttgc
    15601 cgcgatcagg cgagacacgg gtattccggt cgcagcagac gagtcagtcc gctcaatcgc
    15661 cgatgcccaa caggtcgtcg agtgcggtgc cgctgactac attaacatca aaatcatgaa
    15721 gaccggtgtc gtggaagccc gcgacatcgc cgccttcacg cttgcatcag gactcaagct
    15781 gatggtcggt gggatggtgg aaaccaggat cgcgatgggg tgctcgttta gtctcgtgct
    15841 ggggatgagc ggattcgaga ttctcgacct ggatacgccg ctactcctcg gatttgatcc
    15901 gatcagcggc ggctaccggt atgagggccc gcggcttatt ccatggaatg aaccgggctt
    15961 ggggatggct gtctctcctt caaccgaatc gatcgtattg acctgaatga tcccgaccac
    16021 ggcactacct attcgctagc gggaagttag tcacttacca acccccatgc ctccaccctt
    16081 tccaagctga atccgccgta tcctgagaat aggaccagag ggaaatattc cctcctacta
    16141 aatcaaaaaa ctcagctgtc ttcatcgcat ttcttacagt tcagatcttc tcctacatgc
    16201 ttttgccgac cagcatcggt cagcacccaa gggcgacttg tcatcggcgg ctgatgccgc
    16261 acgtgctgcc catgtccgca agccaagtcg gccacccagt cgccctgctc atcttgatgg
    16321 tatccgacga tcttctgttc catcactctc caagccgaca gaacgcgaca tttaggcgcc
    16381 aggtgccgaa acttgccatg cgatcgtcag agtctatcga acaagcgttc tgctctcaca
    16441 ccgactggga aaaacgtcca tgtaccagaa ctgtccacat cgaatgatcc ctgaaatgac
    16501 atcccagctt caagcgaccg aaactcgatc aattcgacac gacagctctg ttcgtcgaag
    16561 cggcggacat cgaactgtcc caccacaaag agacgccctg agtccgtcag atagagattg
    16621 cgccggctca ctcccccggt atcaggaaag agctcgctgg ccgccgaaca gccggaagtc
    16681 gtagtgaccg tgagtttgag attataccgg gcaaggaagg gatgggttgc cacgcgggtg
    16741 acggcaacat gatatccagg agggacaatg tccgtcgttg ctgaaactgg aacatcgctg
    16801 ttgcacgcag cggccgcact gatgagtaca acgagcaact ggccacgaaa ccatctgctc
    16861 acttagccgc tgacactttg ggcgcagcgc cgatggcgtc caatcctgcc tgtgcacagg
    16921 catcgtcaag atgggaaccg ggagccccgc cgacgccgat ggctccgacg acctcccctc
    16981 cgatctcgat cggcaaacca ccgccgagta taaggatatt ctcgttcatg tcgcgaagcg
    17041 actgcaacgt cggaactttc atgatgagtt cagccagttc cgtggtcgga cggcgcaggc
    17101 ttgctgctgt gtaggctttt tttctgctgc tctcaaccgt atgaggcccc gcaccatccg
    17161 atcgtcccat ggtgcgaagg acgccggcac gatccacaac cgacacgctg actcgatagc
    17221 catccttctt gcacacatcg agcgccgctt ggactgcttt gccagccatg gagagcggca
    17281 agaccgattc ctttggcaac tcgtcggcag cataaaccat cgtcgcaatc cgaccctctg
    17341 ccagagataa tgttgcaatc cccgccagga aacatccaag aagttgccct cgaagtgtca
    17401 tgaacatatc tccttgttca tacatagatg ccggtcgccc tgaaattcta ggggagacta
    17461 cgaagctctg gatcagctgt caagaggaga acgtgtagga aggatatccg aaagaagagc
    17521 gcaggggcgt cagctccaaa ccacacgctc ttggttgagg aggtagtcga ttacttcgat
    17581 gctgtcgcgc atctgcgttt gggcacgttc cgacatcggt atgatcgccc cgcagatctt
    17641 acccggttca acgtcatcaa gagaggggat gccatcaggg ccgcgccgtt cgagctcttg
    17701 tctgtagctt gccacgctag gcctcctctg ctggtctcaa ttgactcagc aatttctacg
    17761 ccatgattca gcacgcctgt taatcggcgt actgttagct tagcatatgg acgggaggcc
    17821 cctcggatga gatgtgcaag gcactgatta ttatgagttt taatgctctt accgggtagg
    17881 ctgcgctgca ggtggaaggg cgcccaagaa cgggaatgcc ccgactttca caagcggagt
    17941 atcgccccgc aagcggaaat catcatgccc cggatccaca aagaacgggt caccagtgat
    18001 atctgattgt gcttggagtt ccggcgcagg ctgattcggg ctgccaggcc gcatgtaatt
    18061 gccatctgta ttatagaatg cattgaaaga cgtgctggct cgactcgacg acgcgacgag
    18121 gaaccccact cgattgagcc ccacgacatt gccggacgct tcactctggg actggccaag
    18181 gaacgccgct ccccctccgt tcttgaccaa cgtattactg acaagtacgg ccttggccct
    18241 gtcattgacg acgaccgcct caaaaaggct gcgcgtgatg atgttccgtt ccagtcggac
    18301 gttcgatttg cctgccacat tcacgccatg atcgttgtca tgaataaagt tcccgatcaa
    18361 caccgcctgc gaatcggcaa actggacacc ggtcgtttcg ctgccgccga tcgtgcagtc
    18421 ttcaatgcgg gcatcctcga cttgctgact gaataccatg ccgttaattg tgagccgtcg
    18481 cagaagcact ccacggccat tgaacaggcc cactgcatga ccaccatgct cgttgattgt
    18541 catgtcgctg atctcgatgt ttgtcgctcc atagggccac ttgccgacat gcaacgcacc
    18601 gacgacgtct tcgcgaccca gcaaggtgac cttgtcctgc cctgcaccga ccaacttgac
    18661 cccatcttta ctgtggaccg tgacgtcctg ttgatagcgc cccggcttga gaaacacggt
    18721 gtcgcctttc ttggctgtgt ccaccgcttc ctggatggag gtgaaatccc cggagccgtc
    18781 gagtgccacg gtaatcgtgc gaggagcttg caccacaggc gtttcagccc gtcccactcc
    18841 gctcgccaga gggataatcc ccaagaccag agccatataa agggtcatgc gcatagtcta
    18901 cggtcttaca gggagacaac gcgagaagtc aatcggggtt tcttgtgggg ctgaactcca
    18961 catgctattc tcgcccgcga tgatcctcgt cggactgaca ggtggggtgg cgaccgggaa
    19021 aagcaccgtc gcgaagatgt tcaagcaatg cggggcggtt gtcatcgatg ccgatgagat
    19081 tgcacgaact gtcgtcaaac ccggcaaacc ggcctggcgg cagatcgtga aatcattcgg
    19141 cagaagcgtg ctcgctccgg accgcaccat caaccgtcac gcgcttggcg cgatcgtgtt
    19201 tcgccacccg gccaaacgac gtcttctaga gcgaatcgtt cacccccgcg tcgcacggga
    19261 acaagccaag cgcacaaaac aggctgctag acacgatcct cacgccgtgg tgatctacga
    19321 tgtgccattg ctcttcgaag tcgggatcga taagcgcgtg gaccgtacga tcgt
//
LOCUS       DATIFR010000251         1435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_372574, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000251 DATIFR010000000
VERSION     DATIFR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372574"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VLD60_14430"
     CDS             <1..244
                     /locus_tag="VLD60_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HSB46208.1"
                     /translation="SSGKAPEQIVQEKGLTQVSDEGALGKIIDDVVAKNPTQVAQFKE
                     GKQQVLGFLVGQVMKASGGKANPGKVNELLKQKLGA"
     gene            262..>1435
                     /gene="eno"
                     /locus_tag="VLD60_14435"
     CDS             262..>1435
                     /gene="eno"
                     /locus_tag="VLD60_14435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSB46209.1"
                     /translation="MSAIREIKGRQIVDSRGNPTVEAEVTLESGAKGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVANISKVIAPELLGREAFDQVGIDQAMIELDGTKT
                     KGKLGANAILGVSLAVAKAAASETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIVPVGATQFSEAVRMSTEVFHSLKALLKKKGLSTAVGDEGGFAPDLQSNEEA
                     LTLIMQAIEEAGYQPGQEIALALDCAASELYDKNRYVLEAEKNRERSSEDMISYYGKL
                     LDRYPILSIEDGLSEVDWEGWKMLTEKLGKRVQVVGDDIFVTNVEIFAKGIREGIGNS
                     ILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTG"
BASE COUNT          349 a          312 c          505 g          269 t
ORIGIN      
        1 tagcagtggg aaagcgcctg agcagattgt gcaggagaag gggctgacgc aggtgtccga
       61 tgagggtgcg ctgggaaaaa tcatcgacga cgttgtggcg aagaatccaa cgcaagtggc
      121 gcagttcaaa gaagggaaac agcaagtgct gggcttcctc gtcgggcagg tgatgaaggc
      181 gagcggaggg aaggcgaatc cggggaaggt gaatgaattg ctcaagcaga aattgggggc
      241 gtaactggag agggatagcg catgagtgcg atcagggaga ttaagggcag gcagattgtt
      301 gattcgcgcg gcaatccgac ggttgaggcg gaagtcacgc tcgagagtgg cgcgaagggg
      361 agggcggcgg tgccgtccgg tgcgtcaacc ggagagaagg aagccatcga gctgcgcgac
      421 ggcgacaaga agcgctggat gggcaagggc gtctcgaaag ccgtggctaa tatcagcaag
      481 gtgattgccc cggagttgtt ggggagagag gcgttcgacc aggtcggcat cgatcaggcc
      541 atgatcgaat tggacgggac aaaaacgaag ggcaagttgg gtgccaatgc catccttgga
      601 gtctcgctgg ctgtggcgaa ggcggcggcg agtgaaacgg ggcagccgct ctatcgctat
      661 ctcggcggaa cgaatgcgcg tgtgttgccg gttccgttga tgaatatcat caatggcggt
      721 gcgcatgccg ataatcggct cgatctccaa gagttcatga tcgtgccggt cggagcgact
      781 cagttcagtg aagcggttcg catgtcgacg gaggtgttcc attcattgaa ggcgctcttg
      841 aaaaagaagg gcttgagcac cgcggtcggg gatgaaggcg gctttgcgcc ggacctgcaa
      901 tcgaacgaag aagcactgac cctgatcatg caggcgatcg aggaggccgg ctaccaaccg
      961 gggcaagaga ttgcgctggc attggattgt gcggcaagcg aactgtacga caagaatcgg
     1021 tatgtgctcg aagcagagaa aaatcgtgag cgctcctctg aagacatgat cagttattac
     1081 ggtaagcttc tcgatcgcta tccgattctt tctattgagg acggcttgag cgaggtggat
     1141 tgggaaggat ggaagatgct cacggagaag ctggggaagc gtgtccaggt ggtcggcgac
     1201 gatatcttcg tgacgaatgt ggagatcttt gcaaagggca tccgcgaggg aatcggcaat
     1261 tcaattctga tcaagttgaa ccagattggc acgttgacgg aaaccttgga cgccatcgaa
     1321 ctggccaagc gctccggtta tacggcgatc atttcgcatc gatcgggcga aacggaagat
     1381 acgacgattg ccgatgtcgc cgtcgcgacg aacagcggct tgatcaagac cggat
//
LOCUS       DATIFR010000252        13774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_373100, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000252 DATIFR010000000
VERSION     DATIFR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373100"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VLD60_14440"
     CDS             <1..240
                     /locus_tag="VLD60_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46210.1"
                     /translation="VPVSDRSAWALPSALPTKSGLSSRDVTATNGHPVFSLAQIQYRK
                     PSGRMQEAACSQWGDRSNREAETLRGTPGSSQIAQ"
     gene            complement(455..1324)
                     /locus_tag="VLD60_14445"
     CDS             complement(455..1324)
                     /locus_tag="VLD60_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB46211.1"
                     /translation="MGASLRNVHARPTIGALLILFMLVSGCETNPYTGRSQLLMTSVA
                     EEMQMGAQAYSQVKSDPKMKQSQDPREIEPVKRVAARIIEASKRSKYAEMAKQFQWEV
                     TVIKDDKTMNAFALPGGKIAVYTGIFPVAKTEAGLAAVLGHEVTHALARHGAERMSQG
                     QLTNAALEAIGMAVGSSGMNPVLSQGAMAALGAGAQVGVLLPFSRAHESEADYIGILL
                     AADAGYDPRESIHLWERMEQLSGGGGPSEFLSTHPGHETRIARLKTSMPEALAIYQKR
                     QPVPTVELPPVGR"
     gene            1438..2091
                     /gene="rnhC"
                     /locus_tag="VLD60_14450"
     CDS             1438..2091
                     /gene="rnhC"
                     /locus_tag="VLD60_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HSB46212.1"
                     /translation="MPHRAIERIGIDESGKGDYFGPLVIAAVFVDATTQGELALMQVR
                     DSKKISDGRILEMAPDIKTICPHSVIAIGPHKYNELYAKIKNLNRLLAWGHAKALENL
                     LERVSCERAISDQFGDERLILNALQEKGQKIVLEQRTKAESDLAVAAASIVARAEFLL
                     RLKRLSEEIGTTLPKGASPAVELAGKMLVKKYGQERLGTVAKLHFKTTKAVLGETVS"
     gene            complement(2088..2861)
                     /locus_tag="VLD60_14455"
     CDS             complement(2088..2861)
                     /locus_tag="VLD60_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HSB46213.1"
                     /translation="MNQHLSPLIELQKLDLRIMEINEQRRKIPERLHAVEAPLREARQ
                     LLQDTNAALDTLVKERRSHEKDVEAQDAHTDKMRARLTELKTNKEYQAHLFELQMANK
                     KKSEIEDKVLACMEKIEQLQQTAKDVKNRFEAAEKVFAQEKQVLDELDQKMSAELAEL
                     EVQQRDRAAHVEPSLLSRYTKLKAARKDQALAAIKGGMCAGCRLQIPPQLIAEVKRSQ
                     DLHTCPYCHRILYWDGEVSADQKVQDLVKPSDLEVGESV"
     gene            complement(2921..2997)
                     /locus_tag="VLD60_14460"
     tRNA            complement(2921..2997)
                     /locus_tag="VLD60_14460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2961..2963),aa:Ile,seq:cat)
     gene            complement(3099..4913)
                     /gene="rpoD"
                     /locus_tag="VLD60_14465"
     CDS             complement(3099..4913)
                     /gene="rpoD"
                     /locus_tag="VLD60_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSB46214.1"
                     /translation="MAKQELLVEVKKLISIGKEKGFLTYDELNSTLPAEVVSSEQFGS
                     IMTMFGEMDIEIVDAPEGTERQQKNRSGDQEDSGEEVEEAEASEENEKEIDLTPGALS
                     RTDDPVRLYLKEMGSVALLSREGEIEIAKRIEEGKRDIAFVIYGMPMTIEFILSLREQ
                     LKNGKIDVREIVPIQETEEDFEEEEVERDYEELRLKTLEGLNAVRKVSNSLKGLYEKS
                     RQAAKDPAKLKKIKKQIDAIREQVVDKMEAVNLHGVLKDRMVQRVREMAIQIRTAERE
                     IASCQRRLGVGGEAGAELLRKLCREKKDFLAVKRKTGVSEDGLQDIKKVYQAAKSRIR
                     QLETEEALVSGEEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFL
                     DLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETI
                     NKLIRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSH
                     LGDFIEDKKAISPLEAAIRYDLQRQINSALETLTPREEKVLRKRFGIGEATDHTLEEV
                     GQDFEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            complement(4916..6730)
                     /gene="dnaG"
                     /locus_tag="VLD60_14470"
     CDS             complement(4916..6730)
                     /gene="dnaG"
                     /locus_tag="VLD60_14470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSB46215.1"
                     /translation="MGRGLISDDIINQIRDRTDIVEIVGQHVSLTRAGQNLKGLCPFH
                     QEKSPSFTVSPSRQIFHCFGCGAGGNVFTFLTRITGASFPETVRDLGRKVGIEVQESA
                     VQGGPQAAQTGRIEQLNRAAAAWFQQNLREAQGGTEAREYLVRRGIQQATIDRFSIGV
                     APAEWDGLIKSLTKQGFSQGDLASAGLIIARDSGSGFYDRFRARVMFTITDLRKRVVG
                     FGGRVLGEGTPKYLNSPDTALFKKGQTLFALDVAREAVTRTKTVIVVEGYFDAIALHQ
                     AGLTHTVATLGTALTLEHIQVLRRFASKVVLLFDPDAAGERAALRGLDLFVNSGLGVK
                     VVTLPAGEDPDTYVRKEGPESFARLEEQAPSLLDFALEHSLKTAESSTIEGRIRSVDE
                     VLRILQKSEHPIEREERIRLVAERLGINQQRLIERYPALLKQEGRRPVSVTAAFPAAP
                     SYKGVPEERDLVYLLLHGHLTPADLRRVRPETFSVPACRMVVEKALAHLGQDGRVGLR
                     HLLDTIVDDPDCGPLATELSMREEHFDDVRAHVQGCLDTLDRKRAESVLRELIAQLKA
                     AEREGRIEDAQALNARVNELRLQKAGRPATGVLSLVKE"
     gene            complement(6878..7222)
                     /locus_tag="VLD60_14475"
     CDS             complement(6878..7222)
                     /locus_tag="VLD60_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HSB46216.1"
                     /translation="MSDCLFCKMVEKKIPAKIVHEDEHLLAFDDIHPQAPVHTLVIPK
                     KHVAAIQDCGVQDQELLGKLLLICTKVAKQKGLSETGYRLVANTGRDGGQTVFHLHFH
                     IMGGRHMAWPPG"
     gene            complement(7235..7903)
                     /gene="hisIE"
                     /locus_tag="VLD60_14480"
     CDS             complement(7235..7903)
                     /gene="hisIE"
                     /locus_tag="VLD60_14480"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HSB46217.1"
                     /translation="MTQTGTGEFKFDEQGLLPAVVQDWLDGTVLMLGYMNQEAIGKTL
                     STKSVHFWSRSRKKLWEKGETSGNKLLVKELFIDCDRDTILVKAQAMGPTCHTGERAC
                     FFSKLDEQGVSLPGNTPEAFGGILEGVLRTIRDRRANPQPGSYTAKLFEGGHDKILKK
                     VAEEAGEVIIAAKGGKKEEIIYEVADLFFHTLMVLGYHNVALQDIYQELGKRFGKSGL
                     RSEN"
     gene            complement(7900..8688)
                     /gene="hisF"
                     /locus_tag="VLD60_14485"
     CDS             complement(7900..8688)
                     /gene="hisF"
                     /locus_tag="VLD60_14485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSB46218.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVATVYDREGA
                     DELCFLDITASHENRKTIIDVVERTAAHVFMPVTVGGGVRTLDDIRNLLNAGADKVSI
                     NTAVVQRPEFVKEAAERFGTQCIVVAIDAKRGASLNQWEVFTHGGRKPTGLDAIEWAK
                     RMENYGAGEILLTSMDQDGQQTGYDIGLTAAVSERVNIPVIASGGVGTLEHLYDGFMK
                     GKADAVLAASIFHFRTYTIPQAKAYLRERGVPVRLELAAQVARS"
     gene            complement(8691..9410)
                     /gene="hisA"
                     /locus_tag="VLD60_14490"
     CDS             complement(8691..9410)
                     /gene="hisA"
                     /locus_tag="VLD60_14490"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HSB46219.1"
                     /translation="MIVIPAIDLKDGRCVRLRQGDMAAETVYSEDVPSVARKWQQAGA
                     SVIHVVDLNGAVDGEPRNLPQIEAVMKTVSVKVQVGGGIRNMDSVRRYLQAGVSRVVL
                     GTAALTDRAFLKQACKEFPRRILLGLDARDGKVAVKGWTAVSETKAIDLLKELSGYEI
                     GAVIYTDISRDGMLSGPNLPALKEVVAFSSFPVIASGGITRVEDLRAVQSIGPRIEGA
                     IVGKALYDGKLDLGAAVRALG"
     gene            complement(9427..10116)
                     /locus_tag="VLD60_14495"
     CDS             complement(9427..10116)
                     /locus_tag="VLD60_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46220.1"
                     /translation="MMLTLLSGCSGQKVTTKSSSELPRYQIRTMALVPFTSIATPQVR
                     DQAEPYISTPQSVRLSDISISVPSNVEPPPRNTVPVPGYAAEKVTQLFWSRLKMREGV
                     TVSYSSESARASGSMETAKMTPEMAGAALAKLLKVDAALIGQVLVFQERVGSRMGASP
                     PAAVGFEIKAVAADGQVLWIGNYYERQRPMTEDLIGFIGRWAFVTADELAQYGVDEIM
                     KTFPFGTGGKP"
     gene            complement(10214..10825)
                     /gene="hisH"
                     /locus_tag="VLD60_14500"
     CDS             complement(10214..10825)
                     /gene="hisH"
                     /locus_tag="VLD60_14500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSB46221.1"
                     /translation="MIAIIDYGMGNLRSVSKAFEAMGHKSVVTRDAAAIKNASHVVLP
                     GVGAFGDCMANLERFDLVEPIRSTIQSGKPFLGICLGLQLLFTESEEFGMHKGLGILP
                     GRVRRFAVDPSLKVPHMGWNQVNVQRACPLFEGIADGSNWYFVHSYFVDPSDRNITAT
                     TTTYGVPFVSSIWKDNVVACQFHPEKSQAVGLRLIKNFGSWHL"
     gene            complement(11056..11664)
                     /gene="hisB"
                     /locus_tag="VLD60_14505"
     CDS             complement(11056..11664)
                     /gene="hisB"
                     /locus_tag="VLD60_14505"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSB46222.1"
                     /translation="MKKNGSALRQSNLHRATKETDIRVEWTLDGSGQGKIDTGIRFFD
                     HMLELLSKHGFFDLTVQAKGDIDIDEHHTVEDVGIVMGKALHQALGEKAGIKRFGFAS
                     APLDETLAQVTVDLSGRPFLVYNVNLPDRKIKAFDLGLFEDFFQAFVTHGGLNLHVNL
                     MYGRNPHHIMEAIFKATAKALDQATMLEERLAGKVLSTKGML"
     gene            complement(11661..12944)
                     /gene="hisD"
                     /locus_tag="VLD60_14510"
     CDS             complement(11661..12944)
                     /gene="hisD"
                     /locus_tag="VLD60_14510"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSB46223.1"
                     /translation="MKIVGSTDRAFIPTLKKVAARARVQGAVVEKPVKAILQAVERSG
                     DKAVLRFTKQFDKVSLKPDQLKVTPEEIKEAYFHIRKDEGDALRFAAQRITAFHERQR
                     TKTWMYQDGAVTLGQVVTPIDAVGVYVPGGKAVYPSTVLMSAIPAKVAGVQRIVMCTP
                     PQKGGINPYLLVAADIAGVTEIYRIGGVQAIGALAFGTKTIPKVDKIVGPGNIYVATA
                     KRMLYGTVGIDMVAGPSELLVVADDDAKAAHVAADLLCEAEHDEDAQVWLVTTSASLA
                     KDVVSLVESQLKGLQREKIAAKSIARHSVAFVVTTMDEAIDVANEIAAEHLTLSVDEP
                     FDYLEKIRHAGALFLGRHTPPSVADYIAGPNHVLPTGATARFFSALSLHDYTKISNIV
                     HYTKEELSKVKDHLVRLAHIEGFDAHAKSAQSRFS"
     gene            complement(12941..13645)
                     /gene="hisG"
                     /locus_tag="VLD60_14515"
     CDS             complement(12941..13645)
                     /gene="hisG"
                     /locus_tag="VLD60_14515"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSB46224.1"
                     /translation="MLTIALCKGKLIEPTIELFARAGYNAMQLATDSRRLVFPCPEIG
                     MTFLIVRPTDVPTYVEYGAADVGIVGKDVLLEQDSDVYEPLDLGFGACRIAVAALEGE
                     ESRDRLSSKIRVATKYPKITERYFNQRGVPVEIIKLYGSIELAPIVGLADRIVDLVET
                     GSTLKAHDLVEVECIAQSTARLIVNRASLKLKHKAVTELVRKLRAACLKTQRSRQAAA
                     PKLSRKAGRQGKRTRG"
BASE COUNT         2807 a         4355 c         3499 g         3113 t
ORIGIN      
        1 gtgccggtgt cggatcgctc cgcctgggcg ttacccagcg ctctgcccac gaagtccgga
       61 ctttcctctc gtgacgttac cgccacgaac ggtcacccgg tcttctccct cgcgcagatc
      121 cagtatagaa aaccctcggg ccggatgcaa gaagcggcgt gttcgcagtg gggggatcgc
      181 tcaaaccgag aagctgaaac tctccgcggc actcctggtt catcgcagat tgcacagtag
      241 gcctgcgatg ctgcacaaaa acagtcagcg ctctgatcct cctgaaacga ctgaatgaat
      301 tccaagctcg tgaaccatcc ctccacccag tcgactgccg cgagagaaac gttcccataa
      361 aatgcgcctt ctgaactgct tcacgccttc gtcgagtcga caggctagcc agcttgcatg
      421 aaaaacgaga acccaagacc ccagaaggtg accttcagcg tccgacggga ggaagttcga
      481 cagtaggtac aggctgtcgc ttttgataaa tggccaacgc ttcgggcatc gaggtcttca
      541 gccgagcgat tctcgtttca tggcccggat gggtcgacaa aaactccgat ggtccccccc
      601 cgccagagag ctgttccatg cgttcccaga ggtggatgga ctcgcgcgga tcgtagcccg
      661 catctgccgc aagcaagatc ccgatgtaat ccgcctcgga ttcgtgtgcg cgactgaacg
      721 gaagcaatac gcctacttgc gccccggctc ccaacgccgc catcgcgccc tgagacaaaa
      781 ctggattcat cccgctggaa ccgactgcca tacctattgc ttcgagcgcc gcatttgtca
      841 gctgaccttg gctcattctt tcggcaccgt gacgagccaa tgcatgggtg acctcatgcc
      901 ctaagaccgc ggcaagacct gcttctgttt tcgcgacggg gaaaatgccg gtatacacag
      961 cgatctttcc ccccggcagc gcgaaggcat tcatcgtttt gtcgtccttg atcaccgtca
     1021 cttcccattg aaactgcttg gccatctctg catatttgga ccgcttcgat gcctcaataa
     1081 ttctggccgc cacacgcttg accggttcaa tctcacgcgg atcttgcgac tgtttcattt
     1141 taggatcgct cttgacctga ctatacgcct gcgcccccat ctgcatttct tcagcaacgg
     1201 aggtcatgag caactgggac cgcccggtgt acggattcgt ttcacatccg ctcaccagca
     1261 taaacaggat cagcagggcg ccgatcgtcg gcctcgcatg aacgtttctc agagatgccc
     1321 ccatccctct cctcccctcc acactcaccc tcgtgcgacc aaaattgacc gtctcatcat
     1381 ggtctgttag attaccgcgc agtcataaga attgccacag ggacccgcct ggttttgatg
     1441 cctcaccgcg caatcgaacg aatcggaatc gatgaatccg gcaagggtga ctattttggc
     1501 cccctcgtca ttgcagcagt attcgtggat gcgaccacgc aaggcgaact ggccctgatg
     1561 caggtgcgcg acagcaaaaa aatctccgac ggccgtattc tggaaatggc tccggacatc
     1621 aaaaccatct gcccgcacag cgtgattgcg atcggcccgc acaagtacaa cgaactctac
     1681 gccaagatca aaaatctgaa ccggctgctc gcctgggggc acgcgaaagc actggagaat
     1741 ttgcttgaac gggtttcgtg cgaacgggcg atatccgatc aattcggcga cgagcggctc
     1801 atcttgaacg ccctgcagga gaaaggacag aagatcgtcc tggaacaacg taccaaagcc
     1861 gagtcggact tggcggtcgc cgccgcctct atcgtggcac gcgccgaatt cctcctgcgt
     1921 ttaaagcgcc tctcagagga aatcggaacc acattgccga agggtgcctc accggctgtc
     1981 gaactggcgg ggaaaatgct cgtgaagaaa tacgggcagg aacgattggg aactgtggcc
     2041 aagcttcatt tcaagacgac caaggccgtg ctcggcgaaa cagtgagcta gaccgactcg
     2101 cccacttcca gatccgacgg cttgaccagg tcctgaactt tctggtcggc cgacacttcc
     2161 ccgtcccagt atagtatccg atggcagtag ggacaggtgt ggaggtcttg tgatcgctta
     2221 acctcagcga tcagctgcgg aggaatctgc aggcggcatc cggcgcacat gccccccttg
     2281 atcgccgcca gcgcctgatc ctttctggca gctttgagtt tcgtgtagcg actcagaagc
     2341 gacggctcga catgcgccgc acggtcccgc tgctgaacct caagttcagc caactctgcc
     2401 gacatctttt gatcgagttc gtcgagaacc tgtttttctt gggcaaacac tttctcagca
     2461 gcctcaaatc gattcttcac gtctttggcc gtttgctgaa gctgctcgat cttctccatg
     2521 caagcgagca ctttgtcttc aatttccgac tttttcttat tcgccatctg caattcgaag
     2581 aggtgggcct gatattcctt gttcgtcttg agctcagtca ggcgtgcgcg catcttatcc
     2641 gtgtgcgcat cctgcgcctc cacatccttt tcatgggaac gccgctcctt gaccaatgtg
     2701 tcgagggcgg cgttggtgtc ctgaagcagc tgcctcgctt ctcgtaatgg cgcttcgaca
     2761 gcgtgaaggc gttcggggat ctttcgacgt tgctcgttga tctccataat gcggagatcc
     2821 agcttctgca gttcgatgag gggggagaga tgttggttca atgcactcct gttgtatgga
     2881 agtcccacct gccgggccac agtgaccgtg ccggtccttc tggtgggccc actaggactc
     2941 gaacctagga ccagctgatt atgagtcagc cgctctaacc acctgagcta tgggccccga
     3001 tcagggagtc tgcagatgac tcgccgcgcc tgacccgcag acacgtgcat ttgattctag
     3061 gctgacgaga agacggagtc aaattacccc gcactacctc acagactttc gacaaaactc
     3121 cgcagtttct tgctccggct cggatgacgc agcttacgca aggccttcgc ctcgatctga
     3181 cgtatacgct cacgtgtgac ctcgaaatcc tgaccgacct cctccagcgt gtggtcggtc
     3241 gcttcgccga taccaaagcg tttccgcaat actttttcct ccctcggcgt gagcgtttcc
     3301 aacgcgctgt tgatctggcg ttgaaggtcg taccgaatgg cagcttccag cggcgagatc
     3361 gccttcttgt cctcgataaa gtcgcccaaa tggctgtctt cctcttcgcc gatgggtgtt
     3421 tccagtgaaa tcggctcacg cgcgattttc aaaatcttcc gcactttgtc gaggggcaaa
     3481 tccattcgct cggcaatttc ttccggggtc ggttcgcgtc ccagcttctg aaccaggtgg
     3541 cgagacgtgc gaatgagctt gttgatcgtt tcgatcatgt gaacgggaat gcggatcgtc
     3601 cgagcctggt ccgctatggc acgcgtaatc gcctgccgaa tccaccaggt cgcataggta
     3661 ctgaacttgt agcctcgctt gtactcgaac ttatcgaccg ccttcatcag gccgatgttg
     3721 ccttcctgaa tcaagtcgag aaactgcagg ccccggttcg tatacttttt ggcaatgctg
     3781 acgacgagac ggagattggc ctcaaccagt tcggccttgc cgcgcttgac cttttcttcc
     3841 gccacgtcca gatgtttaac cgcatccttg atctcttccc ctgaaactaa ggcctcttcc
     3901 gtttctaact gccggatacg gctcttggcg gcctgataca ccttcttaat gtcctggaga
     3961 ccgtcttccg acacgcccgt ctttcgcttg accgccagaa aatctttctt ctcccggcag
     4021 agcttccgca ggagttcagc ccccgcctct cccccgactc cgagacgccg ctggcagctg
     4081 gcgatttcgc gctccgcggt ccggatctga atcgccatct cccgcacacg ctgcaccatc
     4141 cggtctttga gcacaccgtg caggttgacc gcttccatct tgtcgacgac ctgttcgcga
     4201 atggcgtcga tttgcttctt gatcttcttg agtttcgcag gatctttcgc cgcttgccgc
     4261 gacttttcat agagaccctt cagcgagttc gagaccttgc ggacggcatt caaaccttcc
     4321 agcgtcttca gccgcaattc ctcgtagtcc cgctcgactt cctcctcttc gaagtcctcc
     4381 tcggtttcct gaatcggcac gatctcgcgg acgtcgatct tgccgttctt gagttgctcg
     4441 cgcagcgata agataaattc gatcgtcatc ggcatgccgt agatgacgaa cgcgatgtcc
     4501 cgctttccct cttcgatgcg tttggcgatt tcgatctcgc cctcacggct caagagcgcg
     4561 acgctgccca tttctttcag atagaggcgc accggatcat ccgtgcggct cagcgcgcca
     4621 ggcgtcagat cgatttcctt ttcgttctcc tcgcttgcct cagcctcttc gacctcttca
     4681 ccgctatctt cctgatcgcc gctacggttc ttctgctggc gctcggttcc ctccggagca
     4741 tccacgatct cgatgtccat ctcgccgaac atcgtcatga tgctgccgaa ctgttcggag
     4801 gacacgactt ccgccggcaa cgtgctattc aactcgtcgt aggtgagaaa gcctttctct
     4861 ttcccgatcg agatcagctt cttcacttcc acgagcaatt cttgtttcgc catacctact
     4921 ccttaaccaa agaaagtacg ccagtcgcag gccgaccggc tttctgcagt cgtaactcgt
     4981 tcacacgcgc attcagcgcc tgagcatcct cgatccgccc ctctcgctcg gcggccttca
     5041 actgcgcgat tagctcccgc aagaccgact ccgccctctt tcgatcgagg gtgtcgaggc
     5101 aaccttggac atgcgcacgc acatcatcga aatgctcctc gcgcatcgac aactccgtcg
     5161 caagcggccc acaatccgga tcatccacaa tcgtatcgag gagatggcgc agcccaaccc
     5221 gtccgtcctg gccaagatga gccaacgcct tttccaccac catccggcag gctggaaccg
     5281 agaaggtttc aggccgcact cgccgcaggt cggctggcgt caaatgtccg tgcagcagca
     5341 gatagacgag gtctcgctcc tccggtacgc ctttgtatga cggcgcagcc gggaatgcag
     5401 ccgtgacact gaccggccgc cggccttcct gcttgagcaa cgcaggatat cgttcaatca
     5461 agcgttgctg gttgatgccg agcctctcgg ccacgagccg gatccgttct tctcgttcga
     5521 tcggatgctc gctcttttgc aagatccgca acacttcatc cacactacgg atgcggcctt
     5581 cgatcgtgct cgactccgcg gtcttcaggc tatgctccaa ggcaaaatcc agcagactcg
     5641 gcgcctgctc ctccagacga gcgaaggatt caggaccttc ctttctcaca tacgtgtcgg
     5701 ggtcttcgcc ggccggcagg gtcacgacct tcacgccgag cccgctgttg acgaacaaat
     5761 ccaatcctcg caatgccgcg cgctcgccag cggcatcggg atcgaagagt aaaacgactt
     5821 ttgatgcgaa tcgccgcagc acctggatat gttcgagggt caacgccgtt ccaagtgtgg
     5881 cgaccgtatg ggtcaatccg gcctggtgca gcgcaatcgc atcgaaatac ccttcgacaa
     5941 caatgaccgt ctttgtgcgg gtcacggctt cccgtgccac atcaagcgca aagagcgtct
     6001 gccctttctt aaatagcgcc gtgtccggag agttcaggta tttaggtgtt ccctcgccga
     6061 gcacacggcc tccgaaccct acgacgcgct tgcgcaagtc tgtgatggtg aacatgaccc
     6121 gcgcccgaaa ccgatcgtag aatccactgc cgctgtcccg cgcaatgatc aaccccgccg
     6181 aggccagatc gccctgtgag aacccctgct tggtcagcga cttgatcaac ccatcccatt
     6241 cagccggcgc cacgccgata ctgaatcgat cgatggtcgc ctgctgaatc cctcgacgga
     6301 ccaggtactc gcgagcctcc gttccgccct gtgcttcgcg aaggttctgc tggaaccacg
     6361 ctgcggcagc ccgattcaat tgctcaatgc ggcccgtctg cgcagcctga ggacctccct
     6421 ggacggcgga ctcttgaact tcgatcccaa cttttcgtcc gaggtcacgc accgtttcgg
     6481 gaaaacttgc cccagtaatc ctggtcagaa acgtgaagac atttcctcct gccccgcaac
     6541 cgaagcaatg gaagatttgt cgggaagggc tgacggtgaa tgacggactc ttctcttggt
     6601 ggaatggaca aagccctttg agattctggc ccgctctggt cagactcaca tgttggccga
     6661 cgatctccac gatatccgtc cggtccctga tttgattgat gatatcgtcg gaaatcaggc
     6721 ctcggcccat gggagtcgtg gctcctgttt ccccacttca agatcagtga ggtcggtatt
     6781 cgtaagtaat cgaaaaaatt gaccggtcag actaacagac ggcaaggaaa tctgtcaatg
     6841 ttgaggaggg agcctgagcc gctttccagc cattcatcta gcccggaggc caggccatgt
     6901 gtcgtccacc catgatgtgg aaatgaagat ggaataccgt ctggccgccg tctcgccccg
     6961 tattggccac caaccggtag ccagtttcgg ataagccctt ctgtttcgcg accttcgtac
     7021 aaatgaggag aagcttcccc aggagctctt ggtcttgcac cccacaatcc tgaatcgccg
     7081 ccacatgctt cttcggaatc accagcgtat gcacaggggc ctgcggatgg atgtcgtcaa
     7141 aggccagcaa atgctcgtct tcatgaacga tcttcgcagg gattttcttc tcgaccatct
     7201 tgcagaagag gcaatcgctc ataaccagcc ctcctcagtt ctcagacctg agcccggact
     7261 tgccaaatcg ttttcccagt tcctgataaa tgtcctgaag ggctacattg tgatagccca
     7321 gcaccattaa ggtatggaaa aagagatcgg cgacttcata gatgatttcc tctttctttc
     7381 ctcccttggc cgcaataatc acctcaccgg cctcttcggc cactttcttg agaattttgt
     7441 cgtgcccgcc ttcgaacagc ttggccgtgt aggatccagg ctgcggattg gcccgacggt
     7501 ctcggatagt ccgcagcacc ccctcgagaa tcccgccgaa agcctcgggc gtgttccccg
     7561 gcaggctgac accctgttcg tcgagtttag agaagaaaca ggcccgctcc cctgtatgac
     7621 atgtcggccc cattgcctgc gccttcacca aaatcgtatc gcgatcgcaa tctatgaaga
     7681 gttctttcac cagcagcttg ttgccggagg tttcaccctt ctcccagagt ttcttccgcg
     7741 aacgactcca gaagtggacg gacttggtgg aaagcgtctt gccgatcgct tcctgattca
     7801 tgtaaccgag catgagcacc gtcccgtcca gccaatcctg gaccacagcc ggaaggaggc
     7861 cctgctcgtc gaacttgaat tcgcctgttc ccgtctgagt catgatcgcg cgacctgtgc
     7921 ggccagttcg agacgcacgg gcactccgcg ctcacgaaga taggcctttg cctgcggaat
     7981 cgtataggtt cggaagtgaa aaattgaagc agccagcacc gcatcggcct tgcctttcat
     8041 gaacccatcg tacaggtgtt cgagcgtgcc cacccctcct gaggcaataa ccgggatgtt
     8101 cactcgttcg gagactgcag ccgtcaagcc aatatcatag ccggtttgct gaccatcctg
     8161 gtccatgctg gttaatagga tttcgccggc gccgtagttc tccatcctct tcgcccactc
     8221 gatcgcatcg agaccggtcg gcttgcggcc gccgtgcgtg aatacctccc attgattcaa
     8281 ggaagcccct cgtttcgcat caatcgccac gacgatgcat tgcgtaccga accgttcggc
     8341 tgcttccttc acgaactcag gccgctgtac aaccgccgta ttgatgctga ccttgtccgc
     8401 cccggcattc agtaagtttc tgatgtcatc gagcgttctc acccctccac cgaccgtcac
     8461 aggcatgaat acatgtgcgg cggtccgctc gacgacatcg atgatcgtct tccgattttc
     8521 gtgcgaggcc gtgatgtcga gaaagcagag ctcatcggct ccctcgcgat cgtagaccgt
     8581 cgccacttcc actggatcac ccgcgtcccg gagattgacg aaactcacgc ccttgacgac
     8641 gcgaccgtct ttcacatcaa gacaaggaat gatccgtttt gtcagcatgg tcagcccagc
     8701 gctcgaacag ccgcgcccag atcgagcttg ccgtcataga gcgctttccc cacaatcgcc
     8761 ccctcgatgc ggggaccgat cgactgcacg gctcgaagat cttccacgcg ggtaatgccg
     8821 ccggacgcga tgaccggaaa ggacgaaaac gccaccactt ccttcaacgc cggaagattc
     8881 ggcccgctca acatgccgtc ccgtgaaata tcggtataga tcaccgcgcc aatctcatat
     8941 ccggaaagtt ccttgagcaa atcgatggcc ttcgtttcag acaccgctgt ccagcccttc
     9001 accgccactt tcccgtcgcg ggcgtccagg ccaagcagaa tgcgtcgcgg aaattccttg
     9061 caggcctgct tcaagaaagc acgatccgtc agtgccgcag ttcccaacac aacgcgggaa
     9121 acccccgctt gaagatatcg acgcacgctg tccatgtttc tgataccacc gccgacctga
     9181 accttcacac tcaccgtctt catgactgct tcgatttgcg gaagattccg tggctctccg
     9241 tcgacggcgc cgttcaagtc aaccacatga atcacgcttg cgccggcctg ttgccatttg
     9301 cgtgcgaccg acggcacatc ctcggaatag accgtctccg ccgccatatc tccctggcga
     9361 agtcgcacac aacgcccatc cttcagatct attgcaggaa tcacgatcat gcgagcgcct
     9421 tttccctcac ggctttccgc ccgtaccgaa cggaaatgtt ttcatgattt catcaacacc
     9481 gtactgtgcc agttcgtcgg ctgtgacgaa tgcccatcgc ccgataaatc ctatgagatc
     9541 ttccgtcatc ggccgctgcc gctcgtagta attcccgatc cacagaactt gcccgtccgc
     9601 tgcgaccgct ttgatctcaa accccacggc agctggaggg ctggccccca tgcggcttcc
     9661 aactcgttcc tgaaatacga gaacctgccc gatcaaagcc gcatcaacct tcagcagttt
     9721 ggcaagagcc gccccggcca tctccggcgt catcttggcg gtctccatac tgccggaggc
     9781 ccttgctgac tcgctggaat acgaaactgt gactccctct ctcattttga gtctactcca
     9841 gaataactga gtcacttttt cggccgcata acctggcacg ggcaccgtgt tccgtggagg
     9901 cggctccacg ttcgatggca ccgagataga aatgtcggac agacgtacgc tttgaggggt
     9961 cgagatatac ggctcggcct ggtcacggac ttgcggcgtg gcaatggatg tgaaggggac
    10021 caacgccatc gtccgaatct gataacgtgg gagctccgag gacgacttgg tcgtcacctt
    10081 ctggccactg cagcctgata acagcgtcag catcatcaca agaagcgtga aaccgctgga
    10141 tgaaagatgc gcaataggac acgggccgtg gcacacctga aaccccttac ttctcaccac
    10201 ttccccctca ctgttacaaa tgccatgacc cgaagttctt gatcagtcgc aacccgaccg
    10261 cttggctttt ttccggatgg aactggcagg ccacgacatt atccttccag atactcgaca
    10321 caaacgggac accataggtc gtcgtggtgg ccgtgatgtt gcgatcggac ggatcgacga
    10381 aataggaatg cacaaagtac cagtttgaac cgtccgcaat cccctcgaac aacggacagg
    10441 ctcgctgcac gttcacttga ttccatccca tgtgcggcac tttcaatgac ggatcaacgg
    10501 caaatcgccg aaccctgccg ggaagaatgc caagcccttt gtgcatcccg aactcttcac
    10561 tttccgtaaa cagcaattgg agccccaaac aaattccgag gaatggtttt cccgattgaa
    10621 tggtggagcg tatgggttca accaaatcga accgctccag gttcgccatg caatcgccga
    10681 atgctccgac gccggggagc acgacatggc ttgcattctt gatcgccgcc gcatcacgtg
    10741 tgaccacgga cttgtgcccc atcgcctcaa aggccttgga gacactccgc aaattgccca
    10801 tgccgtagtc gatgatcgcg atcataacca cttcgtggag gccagaggct cgaggctagt
    10861 ggcaagaggg aacatcttcg ctcccttcca acctattgcc tattgcctct cacctctcgc
    10921 cccatgccta gccgtaaggc cgtaacaata ccgcaaccgg ctctatcccg ccactcccct
    10981 ctttgaaaag aaaagggggc aggctgaatc agcagcctgc cccccaagtt gttcggcgtt
    11041 gccaatttga tttcgctaca gcatcccttt cgtggacagc accttcccgg ccaatcgctc
    11101 ttccaacatc gtcgcctggt ccagcgcttt cgccgtggct ttgaagatcg cttccatgat
    11161 gtggtgcggg ttgcgcccat acatcagatt cacgtgcaga ttgagcccac cgtgcgtgac
    11221 gaaggcctga aagaaatctt cgaacaggcc gaggtcgaac gctttgatct tccgatccgg
    11281 caaattcacg ttgtacacga ggaatggccg gccgcttaga tcaacagtga cctgtgccaa
    11341 ggtctcgtcc agcggtgctg aagcaaaccc gaagcgctta atgccggctt tttcacccaa
    11401 ggcctggtgc agtgctttgc ccatgacgat gccgacatcc tcgactgtgt gatgttcatc
    11461 gatgtcgatg tcgccctttg cctgcacggt gagatcgaaa aagccgtgct tcgaaagcaa
    11521 ttcgagcatg tggtcgaaaa accgaatccc ggtgtcgatt ttcccctgcc cgctgccgtc
    11581 cagcgtccat tcgacacgaa tgtccgtttc cttcgtcgcg cggtgaaggt tcgattgtct
    11641 gagtgccgat ccgtttttct tcatgagaac ctgctttggg ccgacttcgc gtgggcgtcg
    11701 aagccttcga tatgggcgag acgaacaagg tggtccttca ctttgctcaa ctcctccttc
    11761 gtgtagtgca cgatgttgct gatcttcgtg taatcatgga gggacagcgc ggaaaaaaat
    11821 cgagccgtcg cacctgtcgg cagcacatgg ttcggtcccg cgatatagtc cgccactgac
    11881 ggcggagtat ggcggcccag aaacagggcg ccagcgtgcc ggatcttttc gaggtaatcg
    11941 aacggttcgt ccacggacaa cgtgaggtgt tccgctgcga tctcattggc gacatcgatc
    12001 gcttcgtcca tggtggtgac cacgaacgca accgaatgtc gcgcgatcga cttggccgcg
    12061 atcttctccc gttgcagtcc cttgagttgg ctctccacca aagagaccac atctttggct
    12121 aacgatgcgg aggtcgtcac cagccagact tgcgcatcct catcatgttc agcttcacag
    12181 agcaggtcgg ccgccacatg cgccgccttg gcgtcgtcat ccgctacaac cagcaactca
    12241 ctgggtcctg ccaccatgtc aatgccgacg gtgccataca acatccgctt ggccgtcgcg
    12301 acatagatgt ttccggggcc gacaatcttg tcgacctttg gaatggtctt cgtgccgaag
    12361 gccagggcac cgatcgcctg aactccgcca atgcgataga tctccgtcac cccggcgatg
    12421 tccgccgcca ccagcagata ggggttgatc ccgccttttt gaggcggtgt acacatcacg
    12481 atccgttgaa ccccggcaac cttggccgga atggcgctca tcagcaccgt cgaggggtat
    12541 accgccttcc ctccgggaac atagactccc accgcatcaa tcggtgtcac tacctgccct
    12601 aacgtcaccg cgccgtcctg atacatccag gtcttggtcc gctggcgctc atgaaaggcg
    12661 gtgattcgct gcgcggcgaa acgcagcgca tccccttcat ctttccgaat atggaagtat
    12721 gcttctttga tttcttccgg agtgactttg agctgatctg gcttgaggga gaccttgtcg
    12781 aactgtttgg tgaagcgcag aacggcctta tcgccgcttc gttcgacggc ttgcaggatc
    12841 gccttcaccg gtttctcgac caccgcccct tgtacccggg cacgtgcagc cactttcttg
    12901 agcgtaggaa tgaaggcacg atcggtggag ccgacaattt tcatccgcgc gtccgtttcc
    12961 cttggcgacc cgccttacgc gacaacttcg gtgcagcggc ctgacgagac cgttgcgtct
    13021 tgagacaggc ggcccgaagc ttcctgacca gctccgtcac ggccttgtgt ttcaatttga
    13081 ggctggctct attgacgatc aagcgcgcgg tggactgagc gatgcactcc acctcgacca
    13141 gatcgtgcgc cttcagcgtg ctgccggttt cgaccaaatc gacgatacga tccgccaatc
    13201 cgacaatcgg tgccagttcg atcgagccgt agagcttgat gatttcgacc ggcacgccac
    13261 gctgattgaa ataccgctcc gtgatcttcg gatatttggt ggcgacccgg atttttgacg
    13321 agagccggtc gcgcgattct tctccctcga gcgcggcgac ggcaattcta cacgctccga
    13381 accctaaatc caatggctca tatacatcgc tatcctgctc caacagcaca tctttcccga
    13441 cgattccgac gtccgccgcc ccgtattcca catacgtcgg cacgtcggtg ggcctgacaa
    13501 tgagaaaggt catcccgatc tccggacaag ggaacaccag ccgacggctg tcggtcgcaa
    13561 gctgcatcgc gttatagccg gcccgagcaa acaactcgat cgtcggttca atcaacttcc
    13621 ccttacagag cgcaatggtc agcatcgttc actctttccg tcgttcaatg accgctccca
    13681 acccaagtaa cttctcttcg atccgttcat agccgcgatc cagatgatag acgcgcagga
    13741 cttccgtcgt gccttcggca gccagacctg cgag
//
LOCUS       DATIFR010000253        10590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1138886, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000253 DATIFR010000000
VERSION     DATIFR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138886"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VLD60_14520"
     CDS             <1..727
                     /locus_tag="VLD60_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB46225.1"
                     /translation="ILLKELGSKRSGRGGVVIDQERPTIKKSRVIAHNQQIVRYDIER
                     KGELKPQLQKRILRYVESRIGELTCLVVSDYAKGVVTAGLMAELTRLATLRKVPIIVD
                     PKVEHFGFYKGVTLVTPNHLEATQAAGLHGDGDQTIQEAGEILRQRLGCQCVLVTRGE
                     KGMSLFEGDGVSWHLPTQARQVYDVTGAGDTVVGTLALALSTGASMREAAALANYAAG
                     IVVGMIGTATVSAQQLTEALGSD"
     gene            720..1508
                     /gene="kdsB"
                     /locus_tag="VLD60_14525"
     CDS             720..1508
                     /gene="kdsB"
                     /locus_tag="VLD60_14525"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HSB46226.1"
                     /translation="MTKPTPNVTVVIPARYGSSRFPGKPLAMLGRKPMIQHVYEQAAA
                     CRVVADVLVATDDERIQQAVEQFGGQAILMNGDFRSGTDRVAGVARSRAGRFFVDLQG
                     DEIPVDPELLADLIEPFVESGAEMGTLKRAICSTEDLQNPAVVKVVTDQQGRALYFSR
                     APIPLVRDDLSRQATAGLHYIHVGVYIYTRETLLKLAALPTGVLEDAEKLEQLRALEH
                     GISIKVWETKHESLRVDTPADAVSVSEILRQLELNKQPQTSNRS"
     gene            1555..3156
                     /locus_tag="VLD60_14530"
     CDS             1555..3156
                     /locus_tag="VLD60_14530"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSB46227.1"
                     /translation="MSKFIFVTGGVVSSLGKGLASASIGNLMESRGLKITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTDDGAETDLDLGHYERYTSLTLTKENNYTTGRIYNSVITKE
                     RRGDYLGGTVQVVPHITDEIKQCIMRISQGMDVTIVEIGGTVGDIESLPFLEAIRQMP
                     YDVGRDNVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYIPPE
                     MKAKIAMFCNVEKDAVITAKDVETIYEVPIVFRKEGLDELIVRLLKLETGPPNLREWD
                     AMVQKIKHPKHEISVALVGKYAGLKECYKSLAEALVHGGIDHETRVNINWIESEDVER
                     QGTERILREADGILIPGGFGTRGIEGKITTIRYARERQVPFLGLCLGMQCATIEFARN
                     VAGLTGANSAEFDEASPHPVIHLMLDQHGVSDKGGTMRLGSYLCKLGEGTLAQKMYGV
                     SEVRERHRHRYEFNNAYREQLTAKGLVLSGLSPDGRLVEIVELKNHPWFLATQFHPEY
                     CSRPHRPHPLFSGFIGAALRKKLGH"
     gene            3163..3990
                     /gene="kdsA"
                     /locus_tag="VLD60_14535"
     CDS             3163..3990
                     /gene="kdsA"
                     /locus_tag="VLD60_14535"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HSB46228.1"
                     /translation="MANLVDIGSFKAGQGERPFLIAGPCVIESEQLVMETAGQIAEIT
                     KSLDIPYVFKSSFDKANRTSIRSFRGPGIEKGLEVLAKVKKQLGLPVLTDVHTEEQAT
                     EAGKVVDVLQIPAFLCRQTDLLIAAAKTGKVVNVKKGQFLSPLEMGNAVKKVEECGNR
                     RIVLTERGSSFGYNNLIVDMRSFPILRSFGYPVVFDATHSVQLPGGGGTKSSGQREFV
                     EPLACAAAGAGVDGFFMEVHPNPDEALSDGPNMVPLHQLKSLLERVMRICDAARPRN"
     gene            3963..4991
                     /locus_tag="VLD60_14540"
     CDS             3963..4991
                     /locus_tag="VLD60_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HSB46229.1"
                     /translation="MRRSKTTKLKTNVAAQPSARRGGGSLDEGKRVLAIEARAIQSLI
                     DRLDGGFAKAVDLLLTCKGKVVVSGMGKSGLIGQKIAATMASTGTPAFFLHPAEGIHG
                     DLGMLGRQDVLIAISNSGETQEILQLLPYVERMGIRVVAMTGRMTSTLAKNSDVALDV
                     SVSEEACPMGLAPTASTTATLAMGDALAVALLQKRGFKEEDFAQFHPGGTLGRRLLVR
                     VKDLMHAGADVPQVSETVAGTTAMLEMTAKKLGMTTVIDGSGKLAGIITDGDLRRFIQ
                     RGGDFAKATAGELASRNPKSIGPDDLAAKAVEMMERYSITTLVVTQGEGKIVGVIHLH
                     DLLKNGIV"
     gene            5009..5605
                     /gene="pgsA"
                     /locus_tag="VLD60_14545"
     CDS             5009..5605
                     /gene="pgsA"
                     /locus_tag="VLD60_14545"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HSB46230.1"
                     /translation="MHRVLAAWRAVGQESLNWPNFITVVRILLIPVFVALFITPTPDR
                     SLSAAVIFVIAAVTDMLDGYIARRTGQVTKLGKLLDPIADKLLVLSALILLVNVDRVS
                     ALVAILVIAREVAVTGIRAIAAGEGMIIAAETTGKYKMALQVVAIVLLILEGTGLAEL
                     GNLHLAGIVTLYISLVLGYISGGQYVWSFWKQVVAKGL"
     gene            5858..6466
                     /gene="plsY"
                     /locus_tag="VLD60_14550"
     CDS             5858..6466
                     /gene="plsY"
                     /locus_tag="VLD60_14550"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSB46231.1"
                     /translation="MDEELLCVLMAIAGYLLGAIPFGIVVSKAMDLPDPRTVGSKNVG
                     FTNVLRVSGTKAGVLTLLGDMGKGWVMGWAAMQWLTGETSIMVVAISPILGHLFSPFL
                     GFKGGKGVATALGSVLGLSPSMGLLLLLIWLGAVAIWRYSSGGALAAFGLFPVVAIVN
                     EQRQEFLVFSIIVSTLIWIKHKDNIVRLWKGTEGKIGEKKQE"
     gene            6729..6971
                     /locus_tag="VLD60_14555"
     CDS             6729..6971
                     /locus_tag="VLD60_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46232.1"
                     /translation="MADVIVKRGMAAPATVFLESMGPMNFLGSQALHFFTPIIEFAFN
                     AKEVEQVARLLERRDSGTRLIALIEAKSSVKKASGQ"
     gene            6968..8800
                     /locus_tag="VLD60_14560"
     CDS             6968..8800
                     /locus_tag="VLD60_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HSB46233.1"
                     /translation="MTSSVSNQPKIERPLNVIVATDCGSTTTKAILIEKIGDEYRQTY
                     RGEAPTTVEAPFEDVTRGVLNAIAEIEELSGRKILDGDKIITPCRDAKTGVDIYISTS
                     SAGGGLQMMVTGVVQNMTGESAQRCALGAGAIVIDVLASNDGRLPHEKIERIRSMRPD
                     MILMSGGTDGGAVTHVVEMAEYVAAADPRPRFGITYRLPLVYAGNKEAQGQVKKILGQ
                     KTALEITENIRPVLERENLAPARHKIHDLFLEHVMAQAPGYKNLIEMAGAPIMPTPAA
                     VGVIMETIAKREHLNLIGVDIGGATTDVFSVFEGIFNRTVSANLGMSYSISNVLAEAG
                     LANIMRWVPFTIDEQTLRNRIKNKMIRPTTIPQTLDELQIEHAISREALRLALIHHKS
                     LATGLKGIQQERTISDVFEQQTSGKSLVDMLKLDLIVGSGGILSHAPRRIQSMLMMVD
                     AYEPLGFTMLSVDSIFMMPHLGVLSTINEKAATQVFVRDCMIYLGTCVAPIGQGKNGE
                     LCADYEITFPDGKTINDQLAFGDLRLYPLELGKKAKVTMQPAKQVNLGMGAGVPVTRE
                     VQGGVVGLMLDGRGRPLQLPSEQMARVAALAKWYQAVDLYPKGS"
     gene            8933..10060
                     /locus_tag="VLD60_14565"
     CDS             8933..10060
                     /locus_tag="VLD60_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46234.1"
                     /translation="MAHSYTPGLTVTERTTIRRRRLLPLAGKLLVNQGEPVRSDQAVA
                     RAELPGKVFPINLANQLSIAPDEIKDYLIKNEGDLIQKDEILAENKPLIKWFKTEIRS
                     PVTGTIESLSMVTGQVLLREPPRILDLLAYIDGTVVETIPQQGVVVEATCSLVQGIFG
                     IGGEVWGEIAVAVTSPDEPIMAAHVTPAMKGKIVVGGSFLPSDAMAKAKEVGVAGLVI
                     GGIHDKDLRALLGYDLGVAITGTEQVGFTLILTEGFGTIPMAAKTFKLLSSHAGQKAS
                     ISGATQIRAGVIRPEIIIPQAATATAPAAAQPQREGIRLGDPVRIIRDPLFGKIGEVS
                     ALPPDLTKIPTESDVRVLEVTFADGSRAVIPRTNIEVIEGA"
     gene            10087..>10590
                     /locus_tag="VLD60_14570"
     CDS             10087..>10590
                     /locus_tag="VLD60_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46235.1"
                     /translation="MRDKRSTFEEQRAVLEFDVCHFSWLFLVIVCVSVVFPLVASAQT
                     ALSPPQELLAFDTPNDGGGSLTVSWAPGPSDTPDVRYQIMVGDAGVTDPGALKVVAEF
                     PANTHFVKEAKAAWWTRKAETHWHQFVIRSGKGVELKDHATYAVTVAAIRGEQRVFGP
                     VLQATPAP"
BASE COUNT         2293 a         2829 c         3245 g         2223 t
ORIGIN      
        1 gattctcctg aaagagttgg gttcgaagcg gtccggccgt ggcggtgtcg tcatcgatca
       61 ggagcggccg acgatcaaga agtcgagagt catcgcgcat aaccagcaga ttgttcggta
      121 cgacatcgag cggaaggggg aactgaagcc ccaactccag aagcggattc tgcggtatgt
      181 cgaatccagg ataggtgaat tgacctgtct ggttgtctcg gactatgcca aaggcgtggt
      241 aacggcgggg ttgatggcgg aattgaccag gctggccacg ctgcgaaaag tccctatcat
      301 cgtcgatcct aaagttgagc acttcgggtt ctataaagga gtcaccctcg tcaccccgaa
      361 tcaccttgag gcaactcagg ccgccggtct ccacggtgat ggcgatcaga cgattcaaga
      421 ggccggagaa attctccgcc aacggctggg ctgccagtgc gtgctggtga cccgtggaga
      481 aaaggggatg agtttgtttg agggcgacgg ggtctcctgg caccttccca cccaggcgcg
      541 acaagtctac gatgtgaccg gtgctggcga taccgtggtc ggcactctcg ccttggccct
      601 gtcgaccgga gcctcgatgc gggaggccgc ggcgctcgca aattacgccg ccggaatcgt
      661 cgtcggcatg atcggcaccg cgacggtctc ggctcaacaa ctgacggagg cgctggggag
      721 tgactaagcc gacaccgaat gtgaccgtgg tgattccggc gagatatggc tcttcgcgct
      781 ttcccgggaa acctctggcc atgctcggtc ggaagccgat gattcaacat gtctacgaac
      841 aggctgccgc ctgccgggtc gtagcagatg ttttggtcgc gaccgacgac gaacggattc
      901 agcaagcggt ggagcaattc ggcggacagg cgattttgat gaacggggac tttcgtagcg
      961 ggacggatcg cgtggccggc gtggcccggt cgcgggcagg cagatttttc gtcgatctgc
     1021 agggagacga gattcctgtc gatccggaat tattagccga tctgatcgag ccctttgttg
     1081 agtccggtgc cgagatgggc acgctcaagc gcgccatttg ctccaccgag gatctccaga
     1141 atcccgctgt cgtcaaagtg gtcaccgatc aacagggacg tgcgctgtac ttttcgcgtg
     1201 caccgatccc gttggtccga gacgatctca gccggcaggc caccgcaggg ttgcactata
     1261 tccatgtggg agtctacatc tatacaaggg agacgctcct aaaattggcg gcattgccga
     1321 ccggagtgtt ggaagatgcg gaaaagttgg agcagttgcg agcgctcgag cacggcattt
     1381 ccatcaaagt ctgggaaacc aagcatgagt ctctgcgtgt cgatacgccg gcagatgccg
     1441 tatcagtgag cgaaatcttg cgacagcttg agctgaataa gcagccgcaa acgtcgaatc
     1501 gatcatgacg ggatgcgggt ccgaattatc cgatcgcgca ggatggaggc cacgatgagc
     1561 aaattcatat tcgtcactgg tggcgtcgtc tcatccttgg gcaaggggct ggcgtcggcg
     1621 tccatcggca atttgatgga gagccggggc ctcaagatca ccttcctgaa actcgatccc
     1681 tacatcaacg tcgatcccgg gacgatgaat ccctatcagc atggggaagt ctatgtcacg
     1741 gacgacggag cggagaccga tctggatctc ggtcattacg agcgctatac ctcgttgacg
     1801 ctgacgaaag aaaacaacta tacgaccggc cgcatctaca attccgtcat taccaaagaa
     1861 cggcgtggtg attatctggg cggcaccgtt caggtcgtgc cccatatcac cgacgagatc
     1921 aagcaatgca tcatgcggat ttcgcagggg atggatgtga cgatcgtcga gatcggcggg
     1981 accgtgggcg acatcgagag cctcccgttc ctcgaagcga tccggcagat gccctacgac
     2041 gtcggacgcg ataatgtgct ctacgtgcat ctgaccctcg ttccgtatat tggtgcagcc
     2101 ggggagctga aaacgaagcc gacccagcat tcggtgaaca agctgcgcga aatcggtatc
     2161 cagccgaata tccttctctg ccgcaccgac cgctacatac cgcccgagat gaaggccaag
     2221 atcgcgatgt tctgcaatgt cgaaaaagac gcggtcatca cggctaagga cgtggagacg
     2281 atctacgaag tgccgatcgt cttccggaag gaaggcttgg acgaacttat tgttcggtta
     2341 ttgaagctgg aaaccggtcc gccgaatctt cgcgagtggg atgcgatggt gcagaagatc
     2401 aaacatccca agcatgaaat ctccgtggcg ctcgtcggga aatatgcggg gctgaaggaa
     2461 tgttacaaga gcttggccga agcgctcgtt cacggcggca tcgaccatga aaccagagtg
     2521 aacatcaatt ggatcgagtc cgaggatgtc gagcggcagg ggaccgagcg gattttgcgg
     2581 gaggccgacg ggattctgat ccccggtggt ttcgggacca gagggattga aggaaaaatc
     2641 acgacgatcc gctatgcgcg ggaacggcag gttcctttcc tggggctttg ccttgggatg
     2701 cagtgcgcga cgattgaatt cgcgcggaac gtggccgggt tgaccggggc caatagtgcc
     2761 gagttcgatg aggcgtcacc gcatccggtc attcatttga tgctggatca acatggcgtc
     2821 agcgacaagg gtggcacgat gcggttgggg agttacctct gcaaacttgg agagggcacg
     2881 ctcgcccaga agatgtacgg ggtgagcgag gttcgtgagc ggcaccgcca tcggtatgaa
     2941 ttcaacaatg cctatcgcga acaattgact gcgaaggggc tggtgttgag cgggctgtcg
     3001 ccggacggcc ggttggtcga aatcgtcgag ctcaagaacc atccctggtt cctcgccacg
     3061 cagttccatc ccgaatactg ctcgcgcccg catcgccccc atccgttgtt cagcgggttt
     3121 atcggcgcgg cgctgcggaa gaaattgggg cattaaggag acatggcgaa ccttgtcgac
     3181 atcggttcct tcaaggcggg ccaaggggag cggccattct tgatcgccgg gccttgcgtg
     3241 attgagagcg agcagctcgt gatggaaacg gctggccaga ttgccgaaat cacgaagtcg
     3301 ctcgacattc cctatgtctt caaatcatcg ttcgacaagg ccaaccggac ttcgatcagg
     3361 tcgttccgcg ggcccggaat tgaaaagggg ctcgaagtct tggcgaaggt caaaaagcag
     3421 ctgggcttgc ccgtgctgac ggatgtccat acagaagagc aagcgaccga agcgggcaag
     3481 gtggtcgatg tattgcagat tcccgcgttt ctgtgccggc aaacagattt gctcatagca
     3541 gctgcgaaga cggggaaagt cgtgaacgtg aaaaagggcc agttcctctc tccgctggaa
     3601 atgggcaatg cggtgaagaa agtcgaggaa tgcggcaata gacgcattgt gctgaccgag
     3661 cgaggctcgt cgttcgggta taacaatttg atcgtcgaca tgcgctcgtt cccgattctt
     3721 cgaagtttcg gatatccggt cgtattcgac gccacccata gtgtgcaact gccaggcggc
     3781 ggtggcacga aatccagcgg ccagcgagaa tttgtcgagc ccttggcctg cgccgcagcc
     3841 ggtgccgggg tcgacgggtt ttttatggaa gtccatccga atcccgacga ggctttgtcg
     3901 gacgggccca atatggttcc gctccatcaa ttgaaatcac ttcttgagcg tgtgatgcgg
     3961 atatgcgacg cagcaagacc acgaaattaa agaccaacgt agcggcccag ccctccgctc
     4021 gacgtggcgg tggtagtctc gatgaaggca agcgtgtact ggcgatcgag gcacgcgcaa
     4081 tccaatccct catcgatcgt ctagatggcg ggttcgcgaa agcggtcgat cttctcctca
     4141 catgcaaagg taaagtggtg gtgtccggca tgggaaaatc cggattgatc gggcaaaaga
     4201 ttgccgcgac catggccagc accggcacgc cggcgttctt tctccacccg gcggagggca
     4261 tccatggcga tctggggatg ttgggccggc aggacgtctt gattgcgatt tccaacagcg
     4321 gtgagacaca agaaatcctc cagctcttgc cctacgtcga gcgaatgggc atcagggtcg
     4381 ttgccatgac cgggcgaatg acctccacgc tggccaaaaa cagcgacgtc gcactggatg
     4441 tgtcagtctc cgaagaagcc tgtccgatgg gattggcacc gacggcgagc acgacggcta
     4501 ccctcgcgat gggtgatgca ctcgccgtgg cgctcttgca gaaacgcggg ttcaaagagg
     4561 aagattttgc acagttccat ccgggcggca cccttggtcg gcgtttgttg gtgcgagtga
     4621 aagacctgat gcatgccgga gctgatgtgc ctcaagtctc ggaaacggtt gcgggcacga
     4681 cggcgatgtt ggagatgacg gcgaagaagc tcgggatgac aaccgtcatc gatggaagcg
     4741 gcaagctcgc cggcattatc acggacggcg acttgcgtcg attcattcaa cggggaggtg
     4801 attttgccaa ggcaaccgcc ggtgaattgg cgtcgcgcaa ccccaaatca attggtccgg
     4861 acgatcttgc cgcgaaggca gtggagatga tggagcgcta ttccattaca acattggtcg
     4921 tgacccaagg cgaggggaag atcgtcggcg tcatccatct gcatgatttg ttgaagaatg
     4981 gaattgttta aaggatactg aacgtaccat gcaccgcgta cttgcagcct ggagagcagt
     5041 cgggcaagaa tccctcaact ggccgaattt catcacggtc gtccggattc tgttgatccc
     5101 cgtgtttgtg gccctcttta tcacgccgac accggaccgt tctttgagcg ccgccgtcat
     5161 cttcgtgatc gcagccgtga ccgatatgtt ggacgggtat atcgcccgcc gcaccggtca
     5221 ggttacgaaa ctcggcaagt tgctcgatcc catcgccgat aaactcttgg tactttctgc
     5281 cttgattcta ttggtaaacg tcgaccgggt gagtgcgctg gtcgccatcc tcgtgattgc
     5341 gcgcgaagtg gccgtcacgg ggatccgcgc catcgcggcc ggcgaaggga tgatcatcgc
     5401 agccgaaacc accggcaaat acaaaatggc gctgcaagtc gttgcgattg tgctgctgat
     5461 tctcgagggc acaggcctcg cggagctcgg caacctccat ttggccggaa tcgtgacgtt
     5521 atacatctcc ctggttctcg ggtacatctc gggtggccag tatgtgtgga gtttttggaa
     5581 acaggtcgtg gccaaaggtc tgtagaggat gctgaaaaag cccgccagta ctgaaggcat
     5641 ttatccgttc gccaagaata gctataaggg caaacggttc acacgaagtg cggtatgtac
     5701 ctcctcggcc cttcactcgc tgcggccgtg cctgcggaac ggcgcgtctt ggcgcgccgg
     5761 ggctgggtgg gtgagaacag cggacttttt gagcatcctc caaggatctg ttgagttgcc
     5821 ctgctctccg cacctcctcc cgacaatcct ctgatccatg gacgaagaac tcctctgcgt
     5881 tttaatggcg atagccggct atctgctggg ggccattccc ttcggtatcg tggtgtccaa
     5941 agccatggac ctgccggatc cccgcacggt cggcagcaag aacgttggat tcacgaatgt
     6001 actgcgcgtc tccggtacca aagccggcgt tctcacactc cttggagata tgggcaaagg
     6061 gtgggtaatg ggttgggctg cgatgcagtg gctcactggt gaaacctcta tcatggtcgt
     6121 cgcgatctcg ccgatcttgg gccatctctt ctcgccattt ctaggattca aaggtggaaa
     6181 gggcgtggca acggccttgg gatcggtgtt gggtttgtcg ccctcgatgg gcctgctact
     6241 gcttttgatc tggctcggag cagttgcgat ctggcgttac tcatccggcg gagcgctcgc
     6301 cgccttcgga ctctttccgg ttgtggcgat tgtcaacgaa cagcgacagg agtttctcgt
     6361 cttttcgatc atcgtcagca cactgatctg gatcaaacac aaagacaaca tcgtccggct
     6421 ctggaaggga acggaaggca aaataggaga aaagaagcag gaatagagag cagtgtggca
     6481 tccttttagc tcgctgatca caatccaggg aaatccctcg accgcctcaa ttgactccat
     6541 tccacgtgct ccatataatg ccttccaact tctgttttga cctggtttga cctgcactta
     6601 tgcgcgcctt gtccagggaa cacctcacaa aaagactccg ggatttgcgg gccggtctac
     6661 agcacgcctt tgccacgcaa tctgtccagc aacctttctc cgttgaagac ctcgagttga
     6721 tggaacgcat ggctgacgtg attgtgaagc ggggcatggc ggcccctgcg acggtctttt
     6781 tggaatccat gggcccgatg aattttctcg gcagccaagc cctgcatttc ttcacgccca
     6841 tcatcgagtt tgcattcaac gccaaagaag tggagcaggt cgcgaggttg cttgagcgcc
     6901 gcgacagtgg tacccgcctc attgccctca ttgaagcgaa gtcttcagta aagaaagctt
     6961 cgggtcaatg acttcttctg tgtcgaacca gcccaagatc gagcgtcccc tcaatgtgat
     7021 tgtcgccacg gattgcggca gtaccaccac aaaagccatt ctgattgaaa agatcggaga
     7081 cgagtatcgg cagacctatc gtggtgaagc gccgacgacc gtggaagcac cgtttgaaga
     7141 cgtgacgcga ggcgtattga acgccattgc ggagatcgaa gagttgtcgg gtcgcaagat
     7201 cctggacggg gacaagatca tcaccccctg ccgcgatgcg aagaccggtg tcgatatcta
     7261 tatttccacg agcagcgcag ggggcggtct gcagatgatg gtgacgggtg tggtgcagaa
     7321 catgaccggc gaaagtgcgc agcgatgcgc acttggcgct ggcgcgatcg tgatcgacgt
     7381 gctggcctcg aacgacggac ggttgccgca cgagaagatc gaacgcatcc gttccatgcg
     7441 gccggacatg atcttgatgt ccggcgggac ggatggcggg gccgtgacgc acgtggtgga
     7501 gatggcggag tacgtcgccg cggctgaccc tcgcccgcgt ttcggcatca cctaccggtt
     7561 gccgctcgtc tatgccggca ataaagaggc gcaaggacag gtcaagaaga tcctcggcca
     7621 gaaaaccgcg ctagagatca ccgagaacat tcggcctgta ttggagagag agaatctcgc
     7681 acccgcccgt cacaagattc acgatctctt tcttgaacac gtgatggctc aggcccccgg
     7741 ctacaaaaac ctcatcgaaa tggccggcgc gccgatcatg ccgactccgg cggcggtcgg
     7801 cgtgatcatg gagacgatcg cgaagcggga gcatttgaat ttgatcggcg ttgatatcgg
     7861 cggggccacg acggacgtgt tctccgtctt cgaggggata ttcaaccgca cggtcagcgc
     7921 caacctcgga atgtcctata gcatttccaa cgtgctagcc gaggcagggc ttgccaatat
     7981 catgcgttgg gtcccattca cgattgatga gcagacgctc cgcaatcgca tcaaaaataa
     8041 aatgatccgg ccgacgacga ttccacagac gctggatgag ctgcagatcg agcatgcaat
     8101 ctcacgcgaa gcccttcggc ttgcgttgat ccatcataaa tccctcgcaa ccggcttgaa
     8161 aggcatacag caggaacgga cgatttctga tgtgttcgag cagcagactt ccggcaagag
     8221 ccttgttgat atgctgaagc tggacttgat tgtggggagc ggggggattc tctcgcacgc
     8281 tccccgccgc atccagtcga tgctcatgat ggtcgatgcc tacgaaccgt tggggttcac
     8341 gatgctttcc gtggacagca tctttatgat gccccatttg ggcgtgctct cgacgatcaa
     8401 cgagaaggca gcaacccaag tcttcgtccg cgattgcatg atttatctgg ggacatgcgt
     8461 cgctccaatc gggcagggga agaacggcga actctgtgcc gactatgaga ttacgttccc
     8521 cgatggcaag accatcaacg atcaactcgc cttcggcgac ctccggctct atccgctcga
     8581 actgggtaaa aaagccaagg tgacgatgca acctgccaag caggtgaatc tcggaatggg
     8641 agccggcgtc cctgtgacgc gcgaggtgca gggcggtgtg gtcggcctca tgttggatgg
     8701 tcgtggtcgt ccgttgcaat tgcccagcga gcagatggcg cgagttgcag ctctggcgaa
     8761 gtggtatcag gcagtggatc tctatccaaa ggggagctga tagcgtatgg cttatggcaa
     8821 tagcaggatg ggaagtggag tgagaaacca cctgcatatt gcgtttggga cgctcctatg
     8881 acccttgcat ctcaaaccat aagctataag ccattcgctc ttacaactag tcatggctca
     8941 tagctataca ccaggcctta ccgtcaccga acgcacaacc attcgccgtc gtcgcctatt
     9001 gccgttggcg ggaaagctct tggtcaatca aggcgagcct gttcgatcag atcaggcggt
     9061 ggctcgtgcg gagctgccgg ggaaggtttt tccgatcaac ttggcgaatc agctcagcat
     9121 cgcaccggac gagatcaagg actatctgat caagaacgag ggggatctga tccagaagga
     9181 tgagatcctg gccgagaaca aaccgctgat caagtggttc aagacggaga ttcgctcgcc
     9241 tgtgacggga acgatcgaat ctctttcaat ggtgaccgga caagttctgc ttcgtgagcc
     9301 gcctcgcatc ctcgatctgc tcgcctatat cgatggcaca gtggtggaga cgattccaca
     9361 gcaaggcgtg gtcgtggaag ctacttgtag tttagtgcag ggcatcttcg gcattggtgg
     9421 tgaggtctgg ggggagattg ccgttgccgt gacatcgccg gatgagccga taatggcggc
     9481 gcatgtgacg ccggccatga agggcaaaat tgtggtggga ggctcgttcc tcccctccga
     9541 tgcaatggcc aaggccaaag aagtgggcgt ggcggggttg gtcatcggtg gcattcatga
     9601 taaagatctt cgcgcgctat taggctatga tctcggcgta gcgattactg gcaccgaaca
     9661 ggtgggattt acgctgatcc tgacagaagg gttcggtacg atccccatgg ctgccaagac
     9721 gttcaagctg ctttcgtccc acgcgggcca gaaggcttcg atctcaggcg cgacacagat
     9781 ccgagccggg gtcattcgtc cggagatcat cattcctcag gcggcaacgg ccactgcgcc
     9841 tgccgcggcg cagccgcagc gagaaggaat tcgtcttggc gacccggtcc gcatcatccg
     9901 cgacccctta ttcggcaaga tcggcgaggt gtcggcgctc ccgccagatc tcacgaagat
     9961 tccgacggag agcgacgtcc gcgtattgga agtgacgttt gccgatggat cacgcgcggt
    10021 gattccccgc acgaacattg aagtcatcga aggcgcatga caatcgtgag gcgcaagggg
    10081 ttaggagtga gggataaaag gtccacattc gaagagcagc gtgctgtgct cgaatttgac
    10141 gtgtgccact tctcatggct ttttcttgtc attgtctgtg tgagtgttgt tttccccctg
    10201 gttgcttcag ctcaaacggc gttgtcgccg ccgcaagagc ttctggcatt cgatactccc
    10261 aatgacggag gcgggagcct gacagtctcg tgggcgcctg gtccgtcaga tactccggat
    10321 gtccgctatc agataatggt gggcgatgcc ggtgtgaccg atccgggggc actcaaggtg
    10381 gtggcggaat tccctgcgaa cacccatttt gtgaaggagg cgaaggcggc ctggtggacg
    10441 agaaaggcag agacacattg gcatcagttt gtgatcagga gcggcaaggg agtcgaactc
    10501 aaagatcacg caacgtacgc cgtaaccgta gcggcgatac gaggcgagca gcgagtgttc
    10561 ggtccggtgc tgcaggcgac cccggcgcct
//
LOCUS       DATIFR010000254        36545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_565212, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000254 DATIFR010000000
VERSION     DATIFR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565212"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..854
                     /locus_tag="VLD60_14575"
     CDS             270..854
                     /locus_tag="VLD60_14575"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB46236.1"
                     /translation="MPRPKEFNPDEALEKAMQVFWHKGYEATSMEDLLTAMDINRGSL
                     YATFGDKRELFLKAMDRYCSGAGIGNRLSILNEPGPAIPLIRRFIGAMLEFGLSDPLR
                     RGCMITNAVMELAPHEKDIARRVAGRLQMAEEAFFTLLTRARQEGELANDKNPRALAR
                     VLVTMMQGTIVMIKAGTPADVVKQTAKTALSILD"
     gene            921..2261
                     /locus_tag="VLD60_14580"
     CDS             921..2261
                     /locus_tag="VLD60_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSB46237.1"
                     /translation="MKLDDHPTVQLVRRTHPEQSFPVSASTLDAVWLRTICLDAGVDD
                     VGFVELARPALADQRDDILRIFPQAQSLISFVVRMNREPIRSPARSVANLEFHHAGER
                     VNDIARKIVAALEARGVRALNPSMGFPMEMNRFPGKIWVISHKPVAVQAGLGQIGIHR
                     NVIHPKFGNFVLLGTIVLDAKISAYDAPIAYNPCLECKLCVAACPTGAIGTDGHFDFS
                     ACYTHNYREFMGGFTDWVEQIADSPNATVYRRRVSDTESASMWQSLSFGANYKAAYCL
                     AVCPAGEEVIAPFLKDRKSFTTNIVAPLQDKIEQIYVVPASDAESHVARRFPQKTVKR
                     VGNGLRPRSIDQFLAGLSNAFQRGKAAGLDATYHFTFTGMQSRQATITIRENTVCLKD
                     GHIGTPHLRVIADSDTWLRFVRKEGSLIWALLTRKVRLKGSPRWLIAFGKCFPS"
     gene            2408..2767
                     /locus_tag="VLD60_14585"
     CDS             2408..2767
                     /locus_tag="VLD60_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB46238.1"
                     /translation="MKYLCLVYVEEQTLSALPRNERRSLSDESMAFCERLQKAGQLLS
                     ASPLHPVETATTVRVREGKASTTDGPFAETKEQLGGFLMIDVPDLNDAIRIASHFPAA
                     RIGSVEVRPMKELGCAD"
     gene            2775..4025
                     /locus_tag="VLD60_14590"
     CDS             2775..4025
                     /locus_tag="VLD60_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB46239.1"
                     /translation="MSDAAVTPTHEKVDRVYRSESRQVLATLIRVLKDFDLAEEALHE
                     AFAAAMEQWPRDGVPANPRAWLVSAGRFKAIDGIRRRARYDATLNELAQEMETVTAEK
                     SDQEDESVEDDRLRLIFTCCHPALSPEAQVPMTLREVCNLTTEEIARAFLTKPATIAQ
                     RIVRAKAKIRDARIPYEVPPASELPERLDSVLHVIYLIFNEGYSASSGTSLTRADLSG
                     EAIRLGRLLSDLLPEPEVIGLLGLMLLHESRRPARTSATGDLVLLENQDRSLWNRDQI
                     EEGITLMERALSSRRIGPYTLQAAIAAVHAEAPSTAETDWPQIVALYDLLSRAEPSPI
                     VELNRAVAVAMRDGPAAGLALIEAILASGELADYHLIHSARADLCRRLGKKADALVSY
                     KRALALTRQEPERRFLERRLAELG"
     gene            4090..4461
                     /locus_tag="VLD60_14595"
     CDS             4090..4461
                     /locus_tag="VLD60_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB46240.1"
                     /translation="MKYLLLVHHNEAAFNEMDEGIRKSMLAESIQLCHHLHDIGQYLH
                     ASPLHPETTAAIVRVRDGKSSVTDGPFMETKEQLAGYFLVQANDLNEAVEIATKIPGA
                     RIGTVEVRPVREIKGLPGESH"
     gene            4590..5015
                     /locus_tag="VLD60_14600"
     CDS             4590..5015
                     /locus_tag="VLD60_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB46241.1"
                     /translation="MRFMIIVKATKDSEAGMMPSEQLLTEMGKFNEELVKSGVMLAGE
                     GLHPSSKGARVRFSGSKRTVIDGPFAETEELIAGFWLWQVNSKEEAIEWVKRCPNPMK
                     GESEIEIRQVFEGEDFGAEFTPELREQEERLRAQIGKKQ"
     gene            5025..5552
                     /locus_tag="VLD60_14605"
     CDS             5025..5552
                     /locus_tag="VLD60_14605"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSB46242.1"
                     /translation="MLKIIGLIMAALIVALLIYAATRPDTFRLQRSITINAPPDRVFP
                     FIKDFHQWPLWSPWEKMDPNMRKTHSGATQGRGAVYEWDGNKNVGTGQSEIVESEPPS
                     TVKIKLDFFKPFEAHNTTEFTLDGRDGPTNVSWAMHGPQPYMMKVMSIIMNSDKVVGG
                     QFETGLGNLKALAEK"
     gene            5930..6529
                     /locus_tag="VLD60_14610"
     CDS             5930..6529
                     /locus_tag="VLD60_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HSB46243.1"
                     /translation="MRFATLVVTTMCIMLTISPALAKDKKGQKPMDQQAMMELWKKLA
                     TPGEPHKLFASLTGSWTTQTKEWMEPGKPPTESTGTAEMKMLLDGRFLYQEYNAQMMG
                     QPFSGIGIDAYDNMSKKYVTAWMDTMGTGIFVMEGTASADGKTITLKGSHPEPGGGKM
                     THRAVWKIVDSNTQTFDMYGAHHGGKEMKMLEISYTRKP"
     gene            6607..7041
                     /locus_tag="VLD60_14615"
     CDS             6607..7041
                     /locus_tag="VLD60_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46244.1"
                     /translation="MQRSIGMLVLALMLTSLSGCSYLFWPRAGDYATQAKGASGVETM
                     INLTNMMEATAAKAKGGKGNDNAFDDLHNQLHALLDSFCGVTEAQAKTPAYDLAVTHK
                     KELIAIFKRLWKFKDDQPQRDQHLDLLSAEAKELRETLQTLK"
     gene            7062..8216
                     /locus_tag="VLD60_14620"
     CDS             7062..8216
                     /locus_tag="VLD60_14620"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB46245.1"
                     /translation="MKSSTPAESGAVTLLIGTRKGAFTLKSDRTRRTWKVSAPIFLGN
                     IIHHVVCDPRDRRTILMAASTGHLGPTLFRSTDAGKTWKESPAPPAFPKMPDGEKGRA
                     VNHTFWLAPCHANEPNAWYAGTSPQGLFRSDDGGITWQPFSYINDDPQYREWMGTVQD
                     GTPDGPKLHSIIVDPRDPKHLYFAMSGGGVHESVDGGKTFSTLVKGLDVVEGFDPTTV
                     TFHDPHCVRLCPSNPDRLYQQNHCGIYRLDRPGKEWVRIGKNMPQKVGDIGFPMVVHP
                     RNADIAWVFPMDGTTVWPRVSPDGKPAAYITRNAGKSWVRQDKGFPRSNAWWTVKRQA
                     MTRDAHEPLGLYLGTTQGEIWTSRNEGATWTCIARHLPEIYSVEAMVLGR"
     gene            8213..8473
                     /locus_tag="VLD60_14625"
     CDS             8213..8473
                     /locus_tag="VLD60_14625"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSB46246.1"
                     /translation="MKVHIPSALQSYTARRSEVQAEGASLADLLAALDQRYPGIRFRI
                     ITEQDTIRPHIRIFVNDEQVQDLTAPLEQEDSIYIVCALSGG"
     gene            8512..9369
                     /locus_tag="VLD60_14630"
     CDS             8512..9369
                     /locus_tag="VLD60_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB46247.1"
                     /translation="MGLQTARQIIEEQRQEWNHVARGWEKWDRFFDEQMSFLNHRLAA
                     DARLRPGLHVLDLGSGTGYPALLDAQTVGPDGSVIGMDLAEQMLEAARRKATRLGLAN
                     VTFRTSDVTALPFESASFDAVTSRFCLMFLPEIQKAAAEITRVLRPGGWVAAAVWSSP
                     DKNPSISLSMEAIRNVVELPPPDPAAPGIFRLAKQGELAGMLQQAGLVDVMEQEFLAE
                     WSYASAEEYYTSLMELAAPIQILMATIAADRLQLVRQLILEAVSRYQRGNRIIFPIAV
                     RMVAARKAS"
     gene            9377..9691
                     /locus_tag="VLD60_14635"
     CDS             9377..9691
                     /locus_tag="VLD60_14635"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HSB46248.1"
                     /translation="MATKEDGFKDFVLDQLADLRGVTCRAMFGGYGLYRNGTFFGIIH
                     KSRLYFKTDQVSAAHYRKLGMKPFKPTARQTLKNYYEVPIEILESPDDLTAWGSQAAQ
                     RP"
     gene            9865..10353
                     /locus_tag="VLD60_14640"
     CDS             9865..10353
                     /locus_tag="VLD60_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46249.1"
                     /translation="MIRLFGSFLLAGLLGFFFGQTWADERPAPLSLWHTVSSPPTADQ
                     PPAPRKPWVLRDREIAIDLQLLQTLKDAAARPHPRVSVELFDGANHELDITSTVSRIN
                     DTAVIRGIFKPPSQGDFIFVASGNLLVGTMQMGNRLYKTEHIANGRLRLLEVDPNKMP
                     PD"
     gene            10422..10973
                     /locus_tag="VLD60_14645"
     CDS             10422..10973
                     /locus_tag="VLD60_14645"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSB46250.1"
                     /translation="MPSTIWTIGHSTRPIDEFTTLLLGQEIRILVDVRTVPRSRHNPQ
                     FNTDTLGKSLEQAGLAYFHMPLLGGLRNAKKDSINVGWRNASFRGYADYMQTNEFWNA
                     LDELMVHGNTARTAIMCAEAVPWRCHRSLIADALLSRDWMVRHIMTQEKIDEHRLTSF
                     AVFEAGRLIYPAPIDQNAPPRLF"
     gene            10978..11349
                     /locus_tag="VLD60_14650"
     CDS             10978..11349
                     /locus_tag="VLD60_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HSB46251.1"
                     /translation="MWYAFSADVVAVIHLVFVLFVLVGGFLVVKWPRVMWLHLPAVAW
                     GALVEFTGWICPLTPFENWLRWQAGQVGYEGDFLLRLLLPLLYPEALTRTTQILLGAI
                     ALTVNLTIYAWLWRQHRRKER"
     gene            11575..12762
                     /locus_tag="VLD60_14655"
     CDS             11575..12762
                     /locus_tag="VLD60_14655"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB46252.1"
                     /translation="MPTDEITQKVSDRYAKAASTGEQMCCPTSYDFADLKSFIPDEVL
                     KISYGCGTPAGLKTVSPGEKVLDIGSGGGIDCFEAARLVGPSGHVIGIDMTDTMLEIA
                     RKNAPIVASNLGYAIPNVEFRKGMADAMPVHDDTIDLIISNCVINLAPDKRKVFREMF
                     RVAKPGGRFTISDIVSDHAVPQYLVHDAGKWGDCLSGALTLSEYMAGMLEAGFLGIHL
                     VTSSPWQVIDGVHFFSVTLTGYRLPKQTTAPTVCYATLRGPFSRVVDERGTSYQRGIP
                     QPIGAESALLLSQPPLARYFVLSQEPLWLDRDDTRWTAVLPAQEPCVWRGHFALLAGP
                     FLEVADDDHHVYRRGEPLEICSKTLTVLEADGYAPHFAIINRAGSAVSGDAVTCSPNG
                     GCC"
     gene            12848..13888
                     /gene="arsS"
                     /locus_tag="VLD60_14660"
     CDS             12848..13888
                     /gene="arsS"
                     /locus_tag="VLD60_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HSB46253.1"
                     /translation="MPLTLLGRHNPLASSTEQLKLLASSAIVPSFETQLHQAGLFPLR
                     ATGITVFQINVGKLCNQTCRHCHVDAGPDRTESMTRETAELCIQALAKTDIPTVDITG
                     GAPELNPNFRWLVEQSRALGRQVMDRCNLSVLLLPSQANLAEFLAANWVEIIASLPYY
                     RASQTNAQRGEGVFEKSVDGLRMLNALGYGQEGTGLTLNLVYNPVGAFLPPKQEAIES
                     QFRNELRNRHGIEFNHLYTITNMPISRFLEFLIVSGNYEGYMERLANAFNPAAAAGVM
                     CRYTISVGWNGTLYDCDFNQMLDLPVDYGAPAHIRDFDPDLLHQRQIVTGNHCYGCTA
                     GSGSSCGGTVTN"
     gene            complement(13897..14310)
                     /locus_tag="VLD60_14665"
     CDS             complement(13897..14310)
                     /locus_tag="VLD60_14665"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSB46254.1"
                     /translation="MKQRVLFLCTGNSARSQMAEGWLRHLAGDRFEVLSAGTHPAGLN
                     HGSVDAMAEVGIDISGHRSKNAMEFLSQPFDYVITVCDRAKESCPVWSGAKQLLHWNF
                     DDPAGIADAYERRRAFSRVRDEIAARIRQFLDEFR"
     gene            complement(14307..15398)
                     /gene="arsB"
                     /locus_tag="VLD60_14670"
     CDS             complement(14307..15398)
                     /gene="arsB"
                     /locus_tag="VLD60_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HSB46255.1"
                     /translation="MDTTRAIASPAHEKQLNPFERYLTVWVGLCMVAGVFLGQATPGL
                     VQTLRGMEFGQGSHINLPIAVLIWLMIIPMMMKVDFASIRHVGERPSGLLITLFVNWV
                     VKPFSMAVFAWFFFRVVFSVWIVQAEADQYIAGAIILAAAPCTAMVFVWSYLTDGDPA
                     YTLVQVSVNDLIMLVLFAPIVGLLVSGASSLSVPFDVLLYSVLAFIVIPLAIGWGLRT
                     WLVQQYGQRWLEDVLLPKFAPITIVALLLTLLFIFAFQAQNIVSKTFHVVLIAIPILF
                     QVYVNAGLAYGLMHRFRVPYGIAAPGALIGASNFFELAVATAIALFGAESGAALATVV
                     GVLVEVPVMLSVCGICNRTRHWFDREAIA"
     gene            complement(15450..15770)
                     /locus_tag="VLD60_14675"
     CDS             complement(15450..15770)
                     /locus_tag="VLD60_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB46256.1"
                     /translation="MPTSVGKHRAQVAEVFHALADETRLEILDQLKDGERCVCELTDA
                     LHTGQSRLSFHLKVLKDAGLILDRPEGRWTYYSINRETLEELTDCVASMKVAGRSVRS
                     STRC"
     gene            15896..16930
                     /gene="hflK"
                     /locus_tag="VLD60_14680"
     CDS             15896..16930
                     /gene="hflK"
                     /locus_tag="VLD60_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HSB46257.1"
                     /translation="MVWDPKDPWSKRSDPLEDVLKQAQSQFKNLIPTGGFKNILLVGI
                     LIFLAWQSAFIVAPDEEGVVKRFGTPVRTVGPGPHMKIPLLETVLQPKVAKLHRVEIG
                     FRTDRQSHQQMIPQEALMLTGDMNILAIEFIVQYKIKEAANFLFNVADIHDTIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQQDTQDLLQRILDQYQAGVQIAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQAIGYRNDILPKAKGESAQTVNQAKGYAQARLNRAQGEA
                     NRFLATLKEYNQAKDTISKRIYIETMEEILPKMEKVIIDGKAGERILPYLPLDRLSKK
                     PPVTTEGHKP"
     gene            16927..17787
                     /gene="hflC"
                     /locus_tag="VLD60_14685"
     CDS             16927..17787
                     /gene="hflC"
                     /locus_tag="VLD60_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HSB46258.1"
                     /translation="MTKQGLTIAVIAVLIGLFVLGASPLFVVDVTQTAIVVQLGKPVR
                     TVTEAGLYVKVPFVQEVTYFDKRLLDYDSDAQDVITQDKKTLLLDNFAKWRIVDPLKV
                     YQNFQSQRGALQRLHDIIYSELRVALGRHDLLEIVSTTRAELMKVVTQRANEKASAYG
                     IEIQDVRIKRADLPQENEKTVFQRMQAEREQQAKQYRAEGAEEAQKIRSEAEKDKEIL
                     LAQAYKESEEIRGAGDAKAFKIYADAYRQDPKFFDFTRSMEAYKKSFDDKSTIIMSPD
                     SEFFHYLKQR"
     gene            complement(17793..19475)
                     /locus_tag="VLD60_14690"
     CDS             complement(17793..19475)
                     /locus_tag="VLD60_14690"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HSB46259.1"
                     /translation="MTDLEIARSYKPLPIQEIGKHLGLAPDELYPYGKYKGKVSLAAL
                     DRLKSKPQGRYILVTAINPTPLGEGKTTTSIGLAMGLCRLGHRAVVTLRQPSLGPVFG
                     IKGGGTGGGRAQVVPMEEINLHLTGDAHAVSASHNLLSAFIDNHLFHGNALGVDPSRL
                     TWPRSLGVSDRALREVILGEGTGNRKGQFVITEASEVMAILALASSKADLRNRLGRIV
                     VGLTTDGKPVCAEELNCAGSMAVLLKEALLPNVVQTLEGTPAFVHTGPFGNIAHGNCS
                     IVSDAMALRCADYVVTEAGFGSDLGAEKFFNVKCRVSGLKPAAAVVVATLRALKLHGD
                     GGTITSGTPLPPSLTGPNQEALARGFANLEQHIANVMAHGIPVVVAVNAFKDDPLTEL
                     EWVCQQSRKAGALEAAISTHHADGGKGSEELALAVIRAAAQKSAFSYLYEQAWPIKRK
                     IETIATGMYGASGVSFEAQAERHIELAEQLGYGELPICMAKTPLSLSHDPALKGRPTG
                     FTVPIKEVRLLAGAGFLTAVCSGIQLMPGLPKKPAGERIDLDPRTGEIVGLS"
     gene            complement(19636..20379)
                     /locus_tag="VLD60_14695"
     CDS             complement(19636..20379)
                     /locus_tag="VLD60_14695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSB46260.1"
                     /translation="MKEVNQRLRVMLGIETPKTGDQVNGRGGEETPLPEGSSFIPAEK
                     SIGLGVGAQQLSAVSGTLAEGQTTQARGVGELDEQQLVNAVKEGLAWLSKETDTQEKT
                     LQELSLAAEQKSSRWAATPSIWPVKGWVTSGFGPRVSPFTEKPAWHDGLDIGAAANAP
                     VQAPAQGRVTSVGFDPKLGNVVKLDHGFGIETLYGHLAKALVKEGQRVRRGDVVGLVG
                     STGLATGPHLHYMVKVNGQTFDPTRYILD"
     gene            21533..21853
                     /locus_tag="VLD60_14700"
     CDS             21533..21853
                     /locus_tag="VLD60_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSB46261.1"
                     /translation="MGFTISPKELKARLDKGDKLVLLDVREQWEFDMAKLNGSTLIPL
                     GTLPQSLSKLSRDSEIIAICHHGMRSADATNFLLQQGFPNVKNLVGGIDAWSAQVDGS
                     VPRY"
     gene            complement(21895..22818)
                     /locus_tag="VLD60_14705"
     CDS             complement(21895..22818)
                     /locus_tag="VLD60_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB46262.1"
                     /translation="MKVLVTGGAGFIGSHLVDRLVQEGHEVIIVDNLATGKRRNINRS
                     ARFYKLDIQSWRLERVFRNERPNVVMHLAAQMDVRKSVEDPMFDAQVNILGTLNVLQQ
                     AVRHGVRKVVFSSSGGAIYGEQEMYPAPESHVTNPLSPYGLSKLCGEQYLSYYQRISG
                     LQVVSLRYANVYGPRQDPEGEAGVVAIFIQKLLNNEQAVINGNGRQTRDFVFVEDVVE
                     ANLAIMGQETQGTYNVGTGVETSINDLFRILVQHTGSACKEVHGPAKKGEQARSVIDS
                     SKLRHELSWEPKMNLTDGLKRTVEYFRERLG"
     gene            complement(22848..23603)
                     /locus_tag="VLD60_14710"
     CDS             complement(22848..23603)
                     /locus_tag="VLD60_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSB46263.1"
                     /translation="MRISKERLDRLLVTQGLVASREVAARTILAGGVIVDGAIVDKPA
                     KLVPLDARIEIEKPAPYVSRSGEKLAAALEAFSIDPNGVIGLDVGCSTGGFTDCLLQR
                     GAKRIYAVDVGYGQIDWKLRQDPRVVLLERTNIRHVDRTLIPEPIELAVIDVSFISLT
                     LVMPSVVPLLRGDATLVVLVKPQFEVGKGQVGTGGIVRDDAQREAVTTKVIDCGARLG
                     LQVQGVLDSPVKGRKGNREILVSFRRQGIINPS"
     gene            complement(23675..23923)
                     /gene="xseB"
                     /locus_tag="VLD60_14715"
     CDS             complement(23675..23923)
                     /gene="xseB"
                     /locus_tag="VLD60_14715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSB46264.1"
                     /translation="MGGVKFEQAMARLEAIVGELEKGDLPLDESLKIFEEGIRLSKSC
                     LKILEDAEHKVEVLVQDKNGKKRLHAFSPDDEADESTS"
     gene            complement(23983..25356)
                     /gene="xseA"
                     /locus_tag="VLD60_14720"
     CDS             complement(23983..25356)
                     /gene="xseA"
                     /locus_tag="VLD60_14720"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSB46265.1"
                     /translation="MTSTKRQTVSGFPKQILTVSELTSLVRASIESEFPDLWLEGEIS
                     NLRLPGSGHVYCTLKDESSQIRAVLFRSSAMRVRFSLQEGMQVIVRGRLTVYEPRGEY
                     QIVLEAVEPKGIGALQLAFEQLKERLAAEGLFDESRKKPLPAFPRTVGVVTSLSGAAI
                     RDILAVFRRRWPTLHIIIAPVQVQGEGAGEQIAEALDVLNAQGAVEVIIVGRGGGSLE
                     DLWSFNEEVVVRAIANSRIPVVAAVGHEIDVTLADFAADVRAPTPSAAAETVVPVLAE
                     VVERLRELAVRGGRAMARHCLFEYRRLDRMMRGLAEIRFRIQAESQRTDELADRLQRK
                     MRLQLAEAREQVRDGQQGLAGLNPIVLVKRGLAMMPQFVKRLERQMAVLSERRRRQIE
                     TTVTQLHNLSPLAILGRGYSILSRVRDGAILRRSQDVRVGEEIIARLSQGELNCTVKR
                     VLPDPSV"
     gene            complement(25360..26139)
                     /locus_tag="VLD60_14725"
     CDS             complement(25360..26139)
                     /locus_tag="VLD60_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HSB46266.1"
                     /translation="MKVLYIGDIMGEPGRRAVARAIPRLVSQRKIDVVVGNGENVAGG
                     FGITPELAEELFGLGLSAITTGNHAWDKKEILDYFPRERRLLRPANYPEGVPGQGSVV
                     VQTPGGEELGILQLMGRAYMPTLDCPFQVAKREMAKLRKRVAAVLVDMHAEATSEKMA
                     MGHYLDGEATAVVGTHTHVQTADEQILPRGTAYLTDIGMTGPLHGVIGVKKELAIEKF
                     LTGMPRRFEVASGPTVFCAALIELDARIGKALSIERIRILD"
     gene            complement(26136..27704)
                     /gene="rny"
                     /locus_tag="VLD60_14730"
     CDS             complement(26136..27704)
                     /gene="rny"
                     /locus_tag="VLD60_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSB46267.1"
                     /translation="MSTSIVVYLLIGIVGALLGVGLFEVVRRSTAGAKRAQEEEQSRH
                     LIQNAQREAENIVKEAKLEAKDLVFQSKADLEKEQKAKLAELSTTEKRLVQREEAIDK
                     RISVLDKRDSDGQRREQELSKREERLTAKEVACAQAEKEHREALERVAGMTADEAKRH
                     LLLEMESQARLDAAGIAKRTLEEARENAEREAREIITTSIQRVVRDYVSESTISVVPI
                     PNDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLERLM
                     HDGRIHPTRIEEIVEKVKVDIDKLMYEEAEKIIFELGLADFHPELIKVLGRLKYRTSY
                     GQNNLYHAREAAYICGIMASELGLDVRLARRGALLHDIGKAVSHEEEGPHAMLGAEIA
                     KKYGESEKIVNAIAAHHEQVEPICPESVLVAAAEALSAARPGARREALESYVKRLEKL
                     ESLAHAQKGVQKAYAIQAGREIRVIVRQEDVTDTESFQLSRDLAKKIEQELTYPGQIK
                     VTVIRESRYVEYAK"
     gene            complement(28286..28582)
                     /locus_tag="VLD60_14735"
     CDS             complement(28286..28582)
                     /locus_tag="VLD60_14735"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HSB46268.1"
                     /translation="MTKTTDVEIYGQRYAIRGEADEAYIRRLAGLVDGQMRQLAEGMN
                     TTTPSKLAVLTALNLAHQLFESEKKRLQGEADVERRMTSLMESIEEQVPTSLFR"
     gene            complement(28575..28829)
                     /gene="zapB"
                     /locus_tag="VLD60_14740"
     CDS             complement(28575..28829)
                     /gene="zapB"
                     /locus_tag="VLD60_14740"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HSB46269.1"
                     /translation="MALDRLDALETRIKDLVKLVHELKKKNAALEDDLKAARQRFAEE
                     SDSNRRWERERGDIRVRIEKVLGDIELLECFEERKEVAFD"
     gene            complement(28909..30984)
                     /locus_tag="VLD60_14745"
     CDS             complement(28909..30984)
                     /locus_tag="VLD60_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSB46270.1"
                     /translation="MGKTLTKEQVHSLKIERLQRLMEQYPSTVWAKRAGLLSGVLLVE
                     RNPAVAIQFLHGAQRDFPVLDDYIRLWIGEASLKLGDAKQAAVILESIPTAVPDSNIL
                     TRAAYRTGEAWYQASSCPEATNWFSKAVALSDKEPGTPQAYLRQGACQLRVNNITEGR
                     ETLRYLWVRFPYTSEAKEAETLLASNLGGEPWTVQPTERFGRAQAYLSQAFHVEAIDE
                     LKQFLAADPQSPRRAEAKLKLGIAQVRLKQYDQARETFRALAKDGASESREAMVWLAR
                     VYLRQGQGDKLLEVVRALPSSPLSAEQKGQILLFAGMWLEDQKKFDEALLRYRQVAKE
                     GDPASQRAEAQWRVGWVYYRTGRYREAAEALRILIEQYDSEFEPQALYWLGRAAEQSQ
                     QPQAQEFYRQVCQRYRYTYYCQLARERATIQEISETTAETASTSSKPSTNGEAVQPVI
                     TRVDIEQQTAYRRAIELKTLGLDSDAAREVAVLTDRYSRDPDVLIALSTLLNEVGAYH
                     HALRMVRTRFRDKLERTGGDIAPSLWNVAYPTGLLPTIKLQGAKGVDPYFIAAIIREE
                     SQYDEKAVSRVGAIGLMQVMPATANNVAQRVGLPAVGREDLFDRETNIRIGVRYVEQL
                     LEQFSGNLIYTIASYNAGPIVVGNWIAQYRSQSQDEFVELIPYQETRLYVKRVLRSYR
                     EYVRLGGHS"
     gene            31283..32380
                     /gene="rlmN"
                     /locus_tag="VLD60_14750"
     CDS             31283..32380
                     /gene="rlmN"
                     /locus_tag="VLD60_14750"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSB46271.1"
                     /translation="MPERSSTTSAPINLLALDEKGLARLVKESGWPAYRTGQILRWLY
                     QRRARTISQMTDLSQADRAKLASSARIGRATDYTVFRSSDQTRKLVLTLEDGLSVETV
                     LIPDEDRLTLCVSTQVGCTLDCGFCLTGTMGLKRNLKPHEIVEQVLTAQDELGPDERI
                     TNMVFMGMGEPLANLEALSEAIRRLTNQTWGLGWSPRRITISTAGLASRLKDVAPLGV
                     NLAISLNATTEDQRASLMPAVSGIASLKTLVAACRRYPLPPTRRLTFEYVLLAGVNDQ
                     ADDARRLVKLVRGIACKINLIPFNPFPGSAFQRPSDRDVLTFQSVVRQAGIDVFIRKS
                     RGRDVLGACGQLGNLSPTSVVRTLTQIESRC"
     gene            32468..33790
                     /locus_tag="VLD60_14755"
     CDS             32468..33790
                     /locus_tag="VLD60_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB46272.1"
                     /translation="MLVSIAPAGASEPHEYILSNGMKVLLVEVPKAPVATVQVWYKVG
                     SRNEVMGRAGLSHMLEHMMFKGTAKYPKGSFSRLIRKNGGMDNAFTSQDFTAYFENLA
                     ADRVELALEMEADRMQGLILDMNELKTERDVVKEERRLRTEDDPQGALVEALFAQAYL
                     SHPYHWPVIGWFGDLDAMTLDDLQRHYDTFYSPNNATLVVVGDIKTETMLPTIKQLFE
                     PIPRGPEPKPVMTMEGEQKGERRFLLKRDAQVPFVMMGYRVPNYTSEDSYALDVLESI
                     LSRGKSSRLYQSLVYEQKLSLAVGAEYGLMQTDPGLFYFYALVSPGQKVDVVEEALHK
                     EIKRLQNEPPTDQELQRAKNQVEAARVFEQDSNFRHAMLLGQAETVGAGWRKVDQFLD
                     RIRSITAKDVQRVARQYLIEDARTVGTLIPLPPKQADAPTATAQPGKP"
     gene            33793..35160
                     /locus_tag="VLD60_14760"
     CDS             33793..35160
                     /locus_tag="VLD60_14760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB46273.1"
                     /translation="MRIPRQTSHPLGRLLACTLALYAMVLASFNAPTLAADIVPTKTV
                     TPNGMTVLVLEQHFLPIVEVHALIKTGSAQDPPDKAGLANLVASLLDEGTTTRTSKQL
                     AEQIDFVGGALEVKASEDFTTASARVLKKDVDLGFTLLADILQRPAFPKPEFERIRSQ
                     ILGEMASDNDDPGHVAMKAFNQLVFHGHPYRWPFNGTEESLAKITHADVLNFYNKEYV
                     PPQVILTIVGDVTAEQVTALVQTHFGAWKKAAPPARTTKKAAPVDRKTVQLIEKDLTQ
                     SNVILGHGGISRTNPDFYAVTVMNYILGAGGFSSRLMDSIRDKQGLVYGIMSHFDARL
                     MPGSFWVNFQTRTEATNQAIQGVLGELKSIREAPVSDQELSEAKSFLMGSFPLRLDST
                     AKLAQVLAQVEFYGLGFEYFSQYPKWIERVTKEDVQRVAKQYLDPQRYALVVVGNVAK
                     AKIKH"
     gene            35189..35995
                     /gene="larE"
                     /locus_tag="VLD60_14765"
     CDS             35189..35995
                     /gene="larE"
                     /locus_tag="VLD60_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSB46274.1"
                     /translation="MATEQLTEKLNDLRRVISDMQSVLVAFSGGIDSTLVLKIAHEQL
                     GERACGVTAVSPTFPTIELETATRIAGEIGARHEIVRTDQLTIPAFVQNDATRCFHCK
                     TDLYQLLEGMRQPRAQWVVDGTNLDDLGDDRPGIKAAREWNVRSPLVEASCSKADVRV
                     IAKMLDLSSWDKPAAACLSSRIPRGTPITIENLRRIEQAEAILQTEGFRQVRVRDHGE
                     IARIEVAEDELARFNQPDRRARISARLREVGFRFICVDLEGYRPGGISLG"
     gene            complement(36014..36520)
                     /gene="atpF"
                     /locus_tag="VLD60_14770"
     CDS             complement(36014..36520)
                     /gene="atpF"
                     /locus_tag="VLD60_14770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSB46275.1"
                     /translation="MPQFDSHFFSSLIFWEVISFAILFFALYKFAFPGILSMLEEREK
                     KIKDSLDQAERHRSDAERKLKEYEAKLSAVGKEAEGILAAAKERAQRLMEENEQRLTT
                     EAERIKGDATREIDHERRKAIQEIRTQTTDLALMVAEKVVQRSLNDADHRRLADEALE
                     ALSKSSPR"
BASE COUNT         8287 a        10810 c         9658 g         7790 t
ORIGIN      
        1 cgaaggcaaa cagcatcacg cccaagaagg gatccttgcc cacatccacg ttttcatagc
       61 cctctcgcaa ctgtcctgtt tggcctacac atgaaggatc ttccttcacc cctcgaaccc
      121 aaacaaggcg agcttctctt aaagatcgtg tgatgatctt ggctgataac ctggtcacca
      181 gatctggtga ccagggggag ggccaatatt tattattgac tgatcgttcc agaattgggt
      241 attctgggct cctcaattga gggaacctca tgccgcgtcc caaagaattc aatccagacg
      301 aagcactcga aaaggccatg caggtcttct ggcacaaggg ttatgaagcc acgtcgatgg
      361 aagatcttct cactgccatg gacatcaatc gcggcagtct ctacgccacc ttcggcgaca
      421 aacgagaact gttcttgaag gcaatggatc gctattgctc tggtgccggc atcggcaacc
      481 ggctctctat cttgaatgaa ccaggtcctg caattccgct gattcgccgg ttcatcggcg
      541 ccatgctgga attcggcctc tcagatcccc tgagacgcgg ctgcatgatc acgaacgccg
      601 tcatggaact ggctccgcac gagaaggata tcgcgcggag agtggcgggg cgattgcaga
      661 tggcagaaga ggccttcttc acactcctta cgcgcgcgag gcaagaggga gaactcgcga
      721 acgataaaaa cccgcgcgcg ctggcacgag tcctggtcac gatgatgcaa ggcacgattg
      781 tgatgatcaa ggctggaacg ccggcggatg ttgtgaagca gacggctaaa acggcgcttt
      841 cgatcctgga ctgaacggcc atctcttttt taggttcgta tctgaagggc cgttcacaac
      901 tgatggctgg agggacttcc atgaaactgg atgaccaccc taccgttcaa ctggtgcgtc
      961 ggacacaccc cgaacagtcc ttcccggttt cagcgagcac actggatgcc gtttggcttc
     1021 gaacgatctg tctcgacgcg ggcgttgacg acgttggctt cgtcgaattg gcacgaccgg
     1081 ccctggccga tcaacgcgac gacatccttc ggatcttccc gcaggcgcag tccctcatca
     1141 gcttcgtcgt gcggatgaac cgcgagccga ttcgtagccc ggcccgatca gtcgccaacc
     1201 tcgagttcca ccacgctggc gagcgtgtga acgacatcgc ccggaagatc gtcgcggcac
     1261 ttgaggcccg aggcgtacgg gccctgaatc catcgatggg attccccatg gagatgaacc
     1321 gcttccccgg caagatctgg gtcatctcac ataaaccggt tgcggtccag gcaggactcg
     1381 gtcagatcgg tatccaccga aacgtgattc atcccaaatt cggtaatttc gtcctcttgg
     1441 gtaccatcgt cctggatgcc aagatcagcg cctacgatgc accgattgcg tacaacccct
     1501 gtctggaatg caagctttgc gtggcggcct gcccgaccgg cgcgatcggc accgacggcc
     1561 atttcgattt ctctgcctgc tacacgcaca actaccgcga attcatgggg gggtttaccg
     1621 attgggtcga gcagattgcc gacagtccga atgcgaccgt gtatcggcga agggtcagcg
     1681 acaccgaatc cgcttccatg tggcaaagcc tgtcgtttgg cgccaattac aaggcggcgt
     1741 actgccttgc agtctgtcca gccggcgagg aggtcattgc cccgttcctg aaggatcgaa
     1801 agtccttcac taccaatatc gtggcaccgc ttcaggacaa gatcgaacag atctatgtcg
     1861 ttccggcgtc cgatgccgaa tcgcacgtgg cccgacgttt tccgcagaaa accgtcaagc
     1921 gagtcggcaa tggacttcgt ccccgctcga tcgatcaatt cttggccggt ctctcgaatg
     1981 cctttcaacg cgggaaggca gcgggtctcg acgcgacgta ccactttaca ttcacgggta
     2041 tgcaaagccg ccaggccact atcacgattc gtgagaacac ggtctgcctt aaggatggtc
     2101 atatcgggac tcctcatctt cgtgtcatcg ctgacagcga tacgtggctc cggttcgttc
     2161 gcaaagaagg tagcctcatc tgggcactgc ttacccgcaa ggttcggctc aagggatcac
     2221 cgcgatggct catcgcattc ggaaaatgtt ttccgtcgta atttcagcag gttcaaattc
     2281 agtattcggg ggaaagcgat ggacgccctc tccccaggag cccccttgtc gatttcctgg
     2341 acccccaacg actacttgat gtggacggtc atttcacatc aacccaactg gtaaaaggag
     2401 agcacccatg aaatacctct gcctcgtgta tgtcgaagag cagacgctca gcgccctccc
     2461 caggaacgag cgacgctcgc tctcggacga atcgatggcg ttctgcgaga gattgcagaa
     2521 agccggccag ctgctgtcag cttctcccct ccatccggtg gaaaccgcga cgaccgtgcg
     2581 tgtccgcgag ggcaaggcct caacaaccga cggtccgttc gccgaaacca aggagcaact
     2641 gggtgggttt ctcatgatcg acgtgccgga cttgaacgat gctatccgca ttgcctccca
     2701 ttttccggcc gcccgcattg ggagcgtgga ggtccgtccc atgaaggaac ttggctgcgc
     2761 ggactgagag cacgatgagc gacgccgccg tcacaccgac gcacgagaag gtagaccggg
     2821 tctaccgctc cgagtcgcgc caggtgctcg ccaccttgat ccgcgtgctc aaggacttcg
     2881 acctggccga ggaagccctg catgaagcct ttgcggccgc catggagcaa tggccgcggg
     2941 atggtgtccc ggcgaatccc cgcgcctggc tcgtgtccgc cggccgcttc aaagcgatcg
     3001 acggaatacg gcggcgcgcc cgctatgacg ccacgctgaa cgagttggcc caggagatgg
     3061 agaccgtcac cgcggagaaa tccgaccagg aggacgagag cgtcgaagac gaccgattgc
     3121 ggctgatctt cacctgctgc catcccgccc tctcgccgga agcgcaagtg ccgatgaccc
     3181 tgcgcgaagt ctgcaacctc acgaccgagg agatcgcgcg ggcgttcctt accaaaccgg
     3241 ccacgatcgc gcaacggatc gtacgtgcca aggcgaagat tcgtgacgcc cgcattccgt
     3301 atgaagtccc gccggcatcg gagttgccgg aacggctgga ttcagtgctg cacgtcatct
     3361 atctgatctt caatgagggc tactccgcct cgtccggcac ctcgttgacg cgagccgacc
     3421 tgtccggcga agccatccga ctcggtcggc tcctgtctga cctattgccc gaaccggaag
     3481 tcataggcct gctcggcctg atgttgctgc atgagtcgcg gcggccggca cgcacatcgg
     3541 cgacgggaga tctcgtcttg ctggaaaacc aagaccgctc gctgtggaat cgtgatcaga
     3601 tcgaagaagg catcactctt atggaacggg cgctgtcgtc gcggcgaatc ggtccctaca
     3661 cactgcaagc cgcgattgct gcagtccatg ccgaagcccc cagcaccgcc gagaccgatt
     3721 ggccacaaat cgtcgcgctc tatgatctgt tgagtcgtgc ggaaccgtcg ccgatcgtcg
     3781 aattgaacag agccgttgcc gtcgcgatgc gcgatggtcc agcagccgga ttggcactca
     3841 ttgaggccat tctggccagc ggcgagctgg ccgactacca tttgattcac tctgctcgag
     3901 cagacctctg ccgaaggctg ggcaagaagg ccgacgctct cgtctcgtac aaacgggctc
     3961 tcgccctgac aaggcaggag ccggagcggc gatttttaga aaggcggctg gccgaattgg
     4021 ggtgagctcg aacctatagc cgactgctga agatgacaac gacaatcagt tgtgaccgga
     4081 ggcatggcga tgaaatatct tttactggtt caccataacg aagcagcctt caacgagatg
     4141 gacgaaggca tacggaaatc gatgttggcc gagtcgattc agctatgtca tcaccttcat
     4201 gacattgggc aatatctaca cgcctcgcct ctgcatccag agacgaccgc tgccattgtt
     4261 cgggtgcgtg atggcaaatc gtcggtcacc gacgggccgt ttatggagac gaaggagcag
     4321 cttgccggct acttcctcgt ccaagccaac gacctcaatg aggccgtcga aattgcgacc
     4381 aagattcccg gtgcccgtat cggaaccgtg gaggttcgac cagttaggga aatcaagggc
     4441 ttaccaggtg agtcgcattg aagatactcg ccttaacctg gcctgtcgaa ttcgggagtc
     4501 cccgttcgac tagctgatag aagccggaat caaccggctg atcgatagac aagcctgggg
     4561 gacagggaga acgcaaaagg agaaccgtca tgcgattcat gatcatcgtg aaggcgacca
     4621 aggattcgga agcagggatg atgccgagcg aacagctgct gaccgagatg gggaagttca
     4681 acgaagagct agtgaaatcc ggtgtgatgc ttgcgggcga agggctccat ccgagctcga
     4741 agggcgcgcg tgtcaggttt tcgggtagca agcgcaccgt gatcgacggg cccttcgccg
     4801 agacggaaga gctgatcgcc ggcttctggt tgtggcaggt gaattcgaag gaagaggcga
     4861 ttgaatgggt caagcgctgc cccaacccga tgaaggggga gtcagagatc gagattcgtc
     4921 aggtatttga gggggaggac ttcggcgccg agttcacgcc tgaattgagg gagcaagaag
     4981 aacgtctgcg cgcgcagatc ggcaagaagc agtaggaggg gtttatgctg aagattattg
     5041 gcctcatcat ggctgcactg atcgttgcgt tgctcatcta tgcagccacg aggccggata
     5101 cgttccgcct ccagcggtcg attacgatca acgcgccgcc ggacagggtc tttccgttca
     5161 tcaaagactt ccaccaatgg ccgctatggt cgccctggga aaagatggac ccgaacatga
     5221 ggaaaacgca cagcggtgcg actcagggcc gaggcgccgt gtatgagtgg gatggaaaca
     5281 agaacgtcgg cacgggacaa tcggaaatcg tcgagtcaga gcctccgtcg actgtgaaga
     5341 tcaagctgga ttttttcaag ccgtttgaag cgcacaatac caccgagttc acgctggacg
     5401 gccgcgacgg ccccacgaac gtctcgtggg ccatgcacgg acctcagccg tatatgatga
     5461 aggtcatgag catcattatg aattccgaca aggtggtcgg aggacagttt gagaccggtt
     5521 tgggcaatct caaagcgctc gctgaaaagt agaagggatc gagacatgca ttctgtcgac
     5581 ggattcgttc tggccgtgcc aaaaagaatc tgaatgccta ggttcgactg gccaagaaag
     5641 caggcaggat ttggtcggag caaggtgccc ttgaatatcg cgagtgcgtg ggagacgatc
     5701 ttaaggtaaa gaagggtctg tcattccccc gcctaggcag actcaagtca ggagagacgg
     5761 cggtgttttc gtgaatcact attgcatcgc gactgcgtga acaccaaggt gatgaaggac
     5821 cggcgtctca aaaagatgtg tggcgagaag tcgatgccgg tcgacaccaa gcgtatgagc
     5881 tacggcggat tcgaggtaat tgattccagt caatcacaag gaggccatca tgcgtttcgc
     5941 aactctcgta gtcacaacga tgtgcattat gctgaccatc tcgccggcct tggccaaaga
     6001 caagaagggc caaaaaccga tggaccaaca agcgatgatg gaactctgga agaaactcgc
     6061 cacccccggc gaaccgcaca agctgtttgc cagtctcacc ggcagctgga cgacccagac
     6121 caaagaatgg atggagccgg gcaagccccc cacggaatcc accggtaccg ccgaaatgaa
     6181 gatgttgctc gatggacgtt ttctctacca ggaatacaac gcccagatga tgggacagcc
     6241 cttctccggc atcggaatcg atgcctacga caatatgagc aagaagtatg tcacggcctg
     6301 gatggacacg atgggcacag ggatcttcgt gatggaaggc acggccagcg ccgacggcaa
     6361 gactatcacg ctgaaagggt cgcaccctga accgggtgga ggaaagatga cgcatcgggc
     6421 tgtctggaag atcgtcgaca gcaataccca gacattcgat atgtacggtg cccatcacgg
     6481 cggcaaggag atgaagatgt tggagatcag ctatacgcga aagccgtgag gacgttgcgt
     6541 tgcccccggt cgggccacgt gcttgactag attatcagag agagcgttca cctaaggagg
     6601 cctcttatgc aacgatcgat tggaatgctg gttttggcgc tgatgttgac cagcctcagc
     6661 ggctgcagct acctgttttg gccccgcgcc ggcgactacg cgacgcaagc gaagggagcc
     6721 agtggcgtcg aaacgatgat taatctcacg aacatgatgg aagccactgc agccaaggcc
     6781 aaaggcggga aaggcaacga caatgcgttc gatgatctcc acaaccagct tcacgccctc
     6841 ctcgattcgt tttgcggagt caccgaggcg caggccaaga caccggccta cgatctcgcc
     6901 gttacacaca aaaaggaatt gatcgcgatt tttaagcggc tgtggaaatt caaggacgat
     6961 caaccccagc gcgaccagca ccttgacctg ttgagtgccg aggcgaagga actccgagag
     7021 acattacaaa ccttgaagta atcaccagac ggaggccggt tatgaaatcg tcaactccag
     7081 cagagagcgg tgccgttaca ctgttgatcg ggacaaggaa aggcgcgttc acactcaaga
     7141 gcgaccggac gagacggact tggaaggtct cagcaccgat attccttggt aacatcatac
     7201 atcatgtcgt ctgtgaccca cgggatcgac gaacgatttt aatggcagcg agcaccggac
     7261 acctcgggcc aacattgttt cgatcgactg atgccggcaa gacatggaag gagtcgcctg
     7321 ctccacccgc atttcccaag atgcctgatg gcgaaaaggg ccgcgcagtg aaccatacgt
     7381 tctggctggc cccttgccat gcgaacgagc cgaacgcctg gtacgccggc acctccccgc
     7441 agggtctgtt ccgctctgat gacggaggaa tcacctggca accattctcc tacatcaatg
     7501 acgatccgca atatcgcgag tggatgggta ccgtgcagga cggcacaccg gacggcccca
     7561 aactgcattc gatcatcgtg gacccgcgcg acccgaagca tctgtatttc gcgatgtccg
     7621 gcggcggcgt gcacgagtcc gtcgatggcg gaaagacgtt ctcgacgctc gtcaaagggc
     7681 tcgacgtggt cgagggtttc gacccgacca cagtgacctt ccacgatccc cattgcgtca
     7741 ggctctgccc cagcaatccg gaccgcctgt accaacagaa tcactgtggt atctaccggc
     7801 tcgaccggcc tggcaaagaa tgggtgcgca taggaaagaa tatgccccaa aaggtcggcg
     7861 atatcggctt tccgatggtg gtgcacccac gcaatgcgga cattgcctgg gtattcccga
     7921 tggacggcac gaccgtatgg ccacgcgtga gccctgacgg aaagccggcc gcctacatca
     7981 cgcgcaatgc cggcaagagt tgggtgcggc aggacaaggg gttccctcga tcgaatgcct
     8041 ggtggactgt caagcggcaa gcgatgacca gagatgcgca tgagccactg gggctctacc
     8101 tgggaacgac gcagggcgaa atctggacca gtcggaacga gggagcaacc tggacctgca
     8161 tcgccaggca tctgcccgaa atctattcgg tggaagcgat ggtgcttgga cgatgaaagt
     8221 gcacatcccc agcgcgttgc aatcatacac ggcacggcgg agtgaggtac aggcggaggg
     8281 tgcgtcactc gccgacctct tggccgcgct ggatcaacgc tatcctggta tccggtttcg
     8341 tatcatcacg gaacaggata cgatcagacc gcacatcaga atctttgtga atgacgaaca
     8401 ggtgcaggat ttgacggcac ctcttgagca agaggatagc atctacatcg tctgcgcctt
     8461 gagcggaggc tgagaggcta tcaaccttcg atccggcacc atagggaaag catgggacta
     8521 cagactgcgc ggcaaatcat cgaggagcaa cgtcaggaat ggaatcatgt tgcccgcggc
     8581 tgggagaagt gggaccggtt cttcgatgaa cagatgtcgt ttctcaatca ccgcctcgcg
     8641 gctgatgcac ggctgcgacc gggcctacac gtgctggacc ttggatcggg aaccggctac
     8701 cccgcgctgc tcgacgctca gactgtcgga cctgacggca gcgtcatcgg aatggatctc
     8761 gccgaacaaa tgctcgaagc cgcaaggcga aaagctaccc gtttggggtt ggccaacgtc
     8821 acattcagaa cgagcgacgt gacggcactg cctttcgaga gcgcctcatt cgacgcggta
     8881 acgagtcgtt tttgcctcat gttcctcccg gaaattcaga aggccgccgc cgagatcaca
     8941 cgggtgttga ggccaggagg ctgggttgcg gccgccgtct ggtcttcacc ggacaaaaat
     9001 ccctcaatca gcctctcgat ggaggcgatc aggaacgtgg tcgaactacc gccgccggat
     9061 ccggctgcgc ccgggatctt tcggctggcg aagcagggag aactcgccgg gatgttgcaa
     9121 caggccggct tggtcgatgt gatggagcaa gaattcctcg ccgaatggtc ctacgcctca
     9181 gcggaggagt actacaccag cttgatggaa ctcgcagcgc cgatccaaat tctgatggcc
     9241 acaattgcag ccgatcggct gcaattggtc aggcaactca tcttggaggc cgtatcgcga
     9301 tatcagcgcg gcaaccggat catcttcccg attgcggtgc ggatggtcgc ggcgcggaaa
     9361 gcgagttgat gccccgatgg caacgaagga ggacggtttc aaagattttg tcctggatca
     9421 actagccgac ctgcgtggcg tcacgtgccg cgcgatgttc ggcggctacg gactctatcg
     9481 aaacggcaca tttttcggca tcattcacaa aagtcggctc tattttaaaa ctgatcaggt
     9541 atccgcggcg cattaccgca agctcggtat gaagccgttc aaacctactg ctcgccagac
     9601 actcaagaat tattacgaag tgccgatcga gatcctggaa tctcctgatg acctcacggc
     9661 gtggggctct caggctgcgc agcgaccatg actgtatcgc gtctcgtgcg gctacgccca
     9721 agaatccacc aagccccatt cacagatctc tcgaacctgc tgatcctgat cggcggcttt
     9781 gcgtccacat tcttgatcac gtgagtctca tccccctgta taatgaacgg accgacggtg
     9841 ggtgtttcac gagggaacgt atctgtgatc cgcctatttg gctctttcct ccttgccgga
     9901 ttgctcggat ttttcttcgg ccaaacctgg gccgatgaac gccctgcacc actgagcctg
     9961 tggcacacgg tctcatctcc accaacagcc gaccagcctc cggcaccgcg aaaaccctgg
    10021 gtactccgtg atcgggagat cgccatcgac ctccaattac tccagacatt aaaggatgcg
    10081 gcagccagac cccatccccg tgtcagcgtc gaactattcg acggagccaa ccatgaattg
    10141 gatatcacct caaccgtttc ccgaattaat gatacagccg tgatccgcgg catatttaag
    10201 ccgccctcac agggagactt catctttgtg gcaagcggca atctccttgt gggaaccatg
    10261 cagatgggca accgacttta caagaccgaa cacatcgcaa acggccgact gcggctcctt
    10321 gaagttgatc ccaataagat gccgcctgat taatccctct gctcttttgc tctttcggtc
    10381 tgtggtgatt ccgttctcgc tgtggcacac tgctactcca gatgccctct acgatatgga
    10441 ccatcggcca ctcgaccaga cccatcgatg agttcactac actcttgctc gggcaggaga
    10501 tccggattct cgtcgatgtc cgaactgttc ctcgctcccg acacaatccg cagttcaata
    10561 ccgatacgtt gggaaagagt ctggagcagg ccggcctggc atattttcac atgcccctcc
    10621 ttggaggctt gcgaaacgcg aagaaagatt ccatcaacgt cggctggcgc aacgcgagtt
    10681 ttcgggggta cgcggattac atgcagacca acgagttctg gaacgcatta gacgagttga
    10741 tggtgcacgg aaacacggcc cgaaccgcca tcatgtgtgc cgaagccgtt ccctggcgct
    10801 gccaccgctc tctgatcgct gacgcgctgc tgtcgcgcga ctggatggtt cgtcacatca
    10861 tgacacagga gaaaattgac gagcatagat tgacatcgtt cgctgtcttc gaagctggca
    10921 ggctgatcta tcccgccccc atcgatcaga atgcccctcc ccggctcttc taatcttatg
    10981 tggtacgcct tctcagccga tgtcgtagca gtcattcacc ttgtcttcgt cctcttcgtc
    11041 ctcgttggcg ggttccttgt cgtgaagtgg ccgcgggtga tgtggctcca tctgccggca
    11101 gtcgcttggg gagccctcgt ggagtttacc ggctggatct gtccgctcac accgtttgaa
    11161 aactggctgc gctggcaggc cggacaggtc ggctacgaag gagactttct tcttcgcctc
    11221 cttcttcctc ttctctatcc ggaagccctg accaggacca cccagatcct gctcggggcc
    11281 atcgcactca cggtaaatct tacgatctac gcctggctct ggcgacaaca tcgcagaaaa
    11341 gagcgctagc cctcctctac acaccttttg tccgattaaa tttgtaatca gccagtcctc
    11401 acagcatttc tgcacaaaac ttcctggcca caatgtaaga acacgatgac aggaacgact
    11461 cagacaatgc cgcggaaagc catgactgcc acgaagaatt cagtggttga cgttgtccct
    11521 gttctcttga tagatggagc actctgccta tgttctctcg actgtgagga tgccatgccg
    11581 acagacgaaa tcacccagaa ggtcagcgat cgctatgcaa aggccgccag taccggcgag
    11641 cagatgtgtt gccccacgag ttatgacttc gccgatctca agtcgttcat tcccgacgaa
    11701 gtactgaaga tctcctatgg ctgcgggacg ccggccgggc tgaaaactgt cagtcccgga
    11761 gagaaggtcc tggatatcgg ttccggcgga gggatcgact gtttcgaagc cgcaagactg
    11821 gtcgggcctt ccggccatgt catcggcatc gacatgaccg ataccatgct ggagattgcg
    11881 cgcaagaacg ctccaatcgt ggcaagcaat cttgggtacg ccattccaaa tgtcgaattc
    11941 cgcaagggga tggccgacgc gatgccggta cacgacgata ccatcgacct gatcatttcc
    12001 aattgtgtca tcaacctggc gccggacaaa cgcaaagtct tccgcgagat gtttcgggtc
    12061 gccaaaccag gcggacgctt caccatctct gatatcgtgt cagatcacgc cgtcccacag
    12121 tacttggttc acgatgcggg gaaatggggg gactgccttt ccggtgcgct cacgctgtcc
    12181 gagtatatgg ccggaatgct tgaggcgggc ttcctcggca tccatctggt gacgtcctct
    12241 ccctggcagg tgatcgacgg agttcacttt ttctccgtca cattgaccgg ttacagactg
    12301 ccgaagcaga caacggcacc gacggtctgc tatgcgacgc tccgaggccc cttcagccgt
    12361 gtggtggacg aacgcggcac aagctatcag cgtggcattc cccaaccaat cggagcggag
    12421 tcggctttgc tattgagcca accgccactt gctcgctact tcgtgctgtc tcaggaacca
    12481 ctctggctcg atcgcgacga tacccgatgg accgcggtct tgccggcgca agaaccttgt
    12541 gtctggcgcg gacactttgc cctgctcgcc ggtcccttcc ttgaagttgc agacgacgat
    12601 caccacgtct atcgccgggg cgaaccgttg gaaatctgct ccaagacctt gacggtgttg
    12661 gaagccgacg gctatgcgcc tcatttcgcg attatcaatc gagccggtag cgccgtcagt
    12721 ggtgacgcag tcacctgttc gccgaatgga ggctgctgtt gatggtgcca atcttgatcc
    12781 gtgatatccc gccgcgcaga ggctctgcgg cactgaagac gctcgcagag gaaacagggg
    12841 catagatatg ccgttgacgc tgctgggccg acataacccg cttgcatcct cgacagagca
    12901 gctgaaactg ctcgcaagct cagccattgt cccgtcattt gagacgcagc tccatcaggc
    12961 cggcctgttt cctctgcgcg ccacgggcat cacggtcttt cagattaacg ttggcaaatt
    13021 gtgcaaccag acttgccgac attgccatgt tgatgcgggt cccgatcgca ctgaaagcat
    13081 gactcgggag acagctgagc tgtgcatcca ggccctggcc aagaccgaca ttccgaccgt
    13141 cgatatcacg ggtggtgcgc cggagttaaa cccgaacttt cgttggttgg ttgagcaaag
    13201 ccgcgcgctt ggacgccagg tgatggatcg atgcaatctc tcggttctgc tgctcccctc
    13261 tcaagcgaac ctggctgagt ttctggccgc caactgggtc gagatcattg cttctctgcc
    13321 ctattaccgt gcgagccaaa caaatgcgca gcgcggcgag ggagtcttcg agaaatctgt
    13381 tgatgggctt cgcatgctta acgccttagg atacggacag gagggaacgg ggctcaccct
    13441 caatctcgtt tacaatccgg tgggggcctt tcttcccccg aagcaagagg ccatcgagtc
    13501 gcagttcaga aatgaactac gaaaccgcca tggcattgag ttcaaccacc tctataccat
    13561 taccaatatg cccatcagcc ggttccttga gtttcttata gtgagcggca actacgaagg
    13621 gtatatggaa cggttggcca acgccttcaa ccccgctgcg gcagcagggg tcatgtgccg
    13681 ttatacgatc tcggtgggtt ggaacggcac gctttacgac tgcgacttca atcagatgct
    13741 ggatttgccc gttgattatg gcgcaccagc ccatatccgc gatttcgatc ctgatctgct
    13801 tcatcagcgc caaatcgtga ccggcaacca ttgctacggt tgcacggcgg gatcggggtc
    13861 ttcctgtggc ggcacagtca ccaattaatt tcatcgtcaa cgaaactcat ccaaaaactg
    13921 ccgaatccga gctgcgatct cgtcccgcac acgtgaaaag gcacgccgcc gttcgtaggc
    13981 gtcggcgatc cctgctgggt catcaaaatt ccaatgaagg agttgtttgg cacctgacca
    14041 gacagggcaa ctttccttgg cacgatcgca gaccgtaatg acatagtcga acggttgcga
    14101 gagaaactcc atggcattct tcgagcggtg accggagata tcaattccga cttctgccat
    14161 cgcatcgacc gatccgtggt tcaggccggc cggatgcgtt cccgcgctca agacttcaaa
    14221 acgatctcct gccagatgcc gcagccaccc ttccgccatt tggctccgag cggaattgcc
    14281 cgtgcagaga aacagaaccc gctgcttcat gcgatggcct cgcggtcgaa ccaatgtctg
    14341 gtcctgttgc agatgccaca gacggagagc atcaccggca cttccaccaa cacgccaacc
    14401 actgtggcca aggccgcgcc ggactctgcg ccgaacaacg caatggcggt ggcgaccgcc
    14461 agctcaaaaa agttgctggc tccgatcaac gcccccggtg cggcgatgcc atagggcacc
    14521 cgaaaccgat gcatcaaccc ataagccaac ccggcattca cgtaaacctg gaatagaatt
    14581 ggtatcgcga tcaggaccac atgaaacgtc ttgctcacaa tattctgcgc ctgaaatgcg
    14641 aagatgaaca gcaacgtcag gagcagggcg acaatcgtga tcggggcaaa cttgggcaac
    14701 agaacatcct cgagccatcg ttgcccatac tgctgaacca gccatgttcg gaggccccat
    14761 ccaatcgcta acggaatgac aatgaaggcg agaacagaat acagcaaaac atcaaaggga
    14821 actgaaagcg acgacgcgcc gctgaccagt agtccgacaa tcggcgcaaa tagaaccagc
    14881 atgatcaggt cattcacgga gacttggacc agagtgtaag ccggatcgcc gtctgtcaga
    14941 taactccaca caaataccat cgccgtgcag ggcgccgccg ccagaatgat cgctcctgcg
    15001 atgtattgat ccgcctcggc ctgcactatc cagactgaaa acacgacacg gaagaaaaac
    15061 caagcaaaga ccgccatgga aaacggcttg acgacccaat tcacaaagag cgtgatgagt
    15121 agccccgatg gacgttcccc cacatggcgg atcgaggcga aatcaacctt catcatcatg
    15181 ggaatgatca tcagccagat caagacagca atcggcagat taatgtgact gccctggccg
    15241 aactccatcc cccgaagagt ctggacaaga ccgggagtcg cttgcccaag aaacacgccg
    15301 gctaccatgc aaagcccgac ccatactgtc aggtatcgct caaagggatt caactgcttc
    15361 tcatgagccg gagaggcaat cgcccgcgtc gtatccatga cactgcctct tattatatca
    15421 acaattcttg atttaacaga caaaaaaact caacaccgtg tgctcgaccg aaccgatctg
    15481 ccggcaacct tcatcgacgc cacgcaatca gtcagttctt cgagagtctc ccggttgatc
    15541 gagtaatagg tccaccggcc ttccgggcgg tctaggatca aaccggcgtc cttgagtact
    15601 ttgagatgaa atgagagacg ggattggccc gtgtggagcg catcagtgag ttcgcagacg
    15661 caacgttcgc catctttgag ttggtcgagg atttccaatc gcgtctcgtc cgctagcgca
    15721 tgaaaaacct ctgcaacttg agctcggtgc tttcctacgg atgtcggcat gcctttctta
    15781 tacatcaatt attcttgatt aatcaacatc cagtcggcac ggttgcgctg cctccggcag
    15841 tctgctaagg tgcgccgtat gtttccatcg gagtgaactt cagcagaacc cctacatggt
    15901 ttgggacccc aaagacccat ggagcaagag aagtgaccca cttgaggatg tcctcaagca
    15961 ggctcagtca cagttcaaga acctcatccc taccggcggc ttcaaaaata ttctcctcgt
    16021 cggcatcctg atcttcctgg cctggcaaag cgcctttatt gtcgcccccg atgaggaggg
    16081 tgtagtgaaa cgcttcggga cgccggtcag gactgtcggt cccggtccgc acatgaaaat
    16141 cccgctgctc gagaccgtac ttcaacccaa ggtcgccaag ctccatcgcg tcgagatcgg
    16201 atttcgaacc gatcggcaaa gccatcaaca gatgattccg caggaagcct tgatgctgac
    16261 cggcgacatg aacattctcg ccatcgaatt catcgttcaa tacaagatta aagaagccgc
    16321 caacttcctg ttcaacgtcg ctgacattca tgacacgatt ggtaaggcgg ccgaggcctc
    16381 gatgcgggag gtgatcggca aaagtaagat cgatgaagcc cttacaaccg gcaaagctca
    16441 aatccagcag gacacgcagg atttgctcca aaggattctc gatcagtatc aggcaggcgt
    16501 gcagatcgcg gccgtgcaac tccaggatgt cgacccgccg gaagctgtcg ctgccgcctt
    16561 caaagatgtg acgaacgcga aggaggatcg ggaaaagctg atcaaccagg cgatcggcta
    16621 ccggaacgac attctcccca aagcaaaagg cgaatccgcg cagacagtca accaggcaaa
    16681 agggtatgcg caagcgcgat tgaaccgcgc ccagggagaa gccaatcgct ttctcgctac
    16741 gttaaaggaa tacaaccaag ccaaagacac gatcagcaag cggatctaca tcgaaacaat
    16801 ggaagagatc ctgccgaaaa tggagaaagt gatcatcgac ggaaaggccg gcgagcgaat
    16861 ccttccctac ttaccgcttg accgcctatc gaagaaacct cctgtaacaa cagagggaca
    16921 caaaccatga cgaaacaggg gctgaccatc gcggtgatcg cggtgttgat cggattgttc
    16981 gttctggggg cttcgccctt gttcgttgtc gatgtgacac aaacggccat cgtcgtccag
    17041 ctggggaaac cggtcaggac cgtgacggaa gcgggactgt atgtgaaggt tccattcgtc
    17101 caggaagtca cgtatttcga caagcggctc ctcgattacg attcggatgc ccaggacgtg
    17161 atcacgcagg acaaaaaaac cctgttgctc gataatttcg cgaaatggcg catcgtggat
    17221 cccttgaagg tctatcagaa cttccaaagc cagcggggtg cattgcagcg gctccacgac
    17281 attatctatt cggagcttcg agtcgcgctc ggccgtcatg atctcttgga aatcgtctcc
    17341 accactcgag cggagctcat gaaagttgtg acgcaacggg cgaacgagaa agcgtcggct
    17401 tatgggatcg agattcagga cgtccgaatc aaacgcgctg atcttccgca agaaaacgaa
    17461 aagacggtgt tccaacggat gcaggcggag cgggaacagc aggcgaaaca ataccgtgca
    17521 gaaggcgctg aggaagcgca gaaaattcgg tccgaagccg aaaaagataa agaaatattg
    17581 ctggcgcagg cgtacaaaga gtctgaagaa attcgcggag cgggtgacgc caaagcgttc
    17641 aaaatctatg cggatgccta caggcaggat cctaagtttt tcgatttcac cagatcgatg
    17701 gaagcctaca agaaatcctt cgatgacaag tcgacaatca tcatgagccc tgattcagag
    17761 ttcttccact acctcaagca acgctgacct tcttacgaga gtccgactat ctcacccgtt
    17821 cgagggtcga gatcgatccg ttcccctgcc ggttttttcg gtagtcccgg cattagctgg
    17881 atcccggagc agacagccgt tagaaatcct gcgccggcga ggagtcgcac ctctttgatc
    17941 ggtacggtaa acccggtggg acggcctttc agcgccggat catgcgagag ggatagcggt
    18001 gttttcgcca tacaaatcgg caactcgcca tacccaagct gttctgccaa ctcaatatgc
    18061 cgttctgcct gtgcctcgaa cgatacccct gaggcgccat acatcccggt ggcaatcgtc
    18121 tcaatcttcc gcttgatcgg ccaagcctgt tcatagagat aggagaaggc tgatttctga
    18181 gcagcggcgc gaatgacggc aagggccaac tcttccgatc ccttgcctcc gtcagcatga
    18241 tgcgtggata tcgcggcctc cagggctccg gcttttcgcg actgttgaca cacccattcg
    18301 agttccgtga gcggatcgtc cttgaaggca ttgaccgcca cgacaacagg aataccatgg
    18361 gccatcacat tcgcaatatg ctgctccaaa ttcgcaaacc ctcgagcaag tgcttcctgg
    18421 ttaggtccag tgaggctggg tggaagtgga gtgcccgacg ttatggttcc cccgtctccg
    18481 tgcagcttga gcgcgcgcag cgtcgccaca acgactgccg cagccggctt gagcccggaa
    18541 acgcggcact tcacgttaaa gaatttctcc gcacccaggt cgcttccaaa tcccgcttca
    18601 gttacgacgt agtccgcaca acgcaaggcc atcgcatctg atacgatcga gcagttgcca
    18661 tgggcgatgt tgccaaatgg accggtgtgg acaaacgccg gcgttccctc caatgtctga
    18721 accacattgg ggagtaaggc ctccttgagc aagacggcca tcgatccggc acagttcagt
    18781 tcctccgcgc aaaccggctt gccatctgtc gtcaacccaa caacaatccg gccaagccgg
    18841 ttcctgaggt cggctttgct ggaagccaac gccaagatgg ccataacctc cgacgcttcc
    18901 gtgatgacga attggccttt tcgatttccc gtcccctccc caaggattac ttcccggagc
    18961 gcccgatcgc tgacccctaa cgatcgaggc cacgtaagcc gagaaggatc aacgcccagg
    19021 gcattcccgt gaaacagatg gttgtcaata aatgcagata acaagttatg gctggcggac
    19081 accgcgtggg catcgccggt caggtggaga ttgatctctt ccatcggaac gacttgtgcg
    19141 cggcctcctc ctgtcccccc tccttttatt ccaaacactg gtcctaatga aggttgccga
    19201 agcgtcacga ccgcacggtg accaagccgg cacagcccca tggccaaacc gattgaagtt
    19261 gtcgttttcc cctctcccaa gggagttgga ttaatcgctg taaccaggat gtagcggcct
    19321 tggggttttg acttgagccg gtctaatgcg gcaagagaaa ctttgccttt gtactttccg
    19381 tacggataca gctcatccgg tgcaagccca agatgcttac caatttcttg gatcggcaag
    19441 ggcttatacg atctagcaat ctcgagatcg gtcatgggtc cagggccagg agatgggatg
    19501 aagagtcggg aacggattat accacggtca tcaaggggga ttgagggcct cgatcaaaag
    19561 aaacgcgcca cgaatcgcat actctcgatc cgaagcgcgt ctttgagact ctagatggga
    19621 aaatcttatc taaggctagt caagtatgta cctggtcggg tcaaatgtct ggccattcac
    19681 tttaaccatg tagtgaaggt ggggacccgt ggctaagccg gtgctcccaa ccaaacccac
    19741 gacatcacca cgcctcacgc gttgcccttc cttcaccagt gccttggcaa ggtggccgta
    19801 caaagtttcg atgccaaacc catggtccaa tttcacaacg ttaccaagtt taggatcaaa
    19861 cccaacagat gtcacacgac cctgtgcagg agcttgcacc ggagcattgg cagctgcgcc
    19921 gatatccagg ccatcgtgcc aggccggctt ctctgtgaac ggggaaaccc tcggaccaaa
    19981 tcctgacgtt acccatccct tgaccggcca aattgaagga gtagctgccc agcgagagga
    20041 cttctgttca gcagcgagcg agagctcctg aagcgtcttt tcctgggtgt ccgtctcctt
    20101 tgaaagccaa gcgagacctt ctttgaccgc gtttactaat tgctgttcat cgagctctcc
    20161 aacaccccta gcctgtgtgg tctgtccctc cgccagagtt ccgctcacag cactgagttg
    20221 ctgtgctccc actccaagcc caatgctctt ctcggctggg atgaaactgc tgccttctgg
    20281 gaggggagtt tcctcaccgc ctcgcccgtt gacctgatcg ccggtcttcg gcgtctcgat
    20341 tcctagcatg actctgagac gctggttgac ctctttcatg ctcgtaaggc gcttcttgag
    20401 gtcatcaaca gctgtcgaga aagccactgt ttgttggcga gcgctcattg cctcatctcg
    20461 gaaagacgaa agttcccata cctgaccagt tctgatcaca taatgtgaaa tgaccagcag
    20521 gtcagcaaca agaagaatag cgccaactat caatagcttg cgaacgaatt tcctggggaa
    20581 gctaaaacga agcggcttcg cagtcgagcc acgaaagata acgactgtgt aggtgtcgct
    20641 ggattctgtc ccgttcactt gtcccatgac cttcaccctc cgctgcaagg ggacctctgt
    20701 ttcctagata gagtctgacg agtttaccca tctaggctgc ttcggtcaac cccctcacct
    20761 tggtaatgaa ctgcgcattg gaacgtcaat atactgtggt ttctcgttcg acccagtcaa
    20821 tatatagtgg caatccacga tcgatccgaa ttgaaataat ctcgggaacc tcatacgaat
    20881 gcagtgaacg aatggccttt tgaagagctg gatatcgtcg ccgagtcgtc tttatgagca
    20941 tgagcatttc tcggcttctc tgaacttttt cctgccacct aaaaattgaa gtaacggaag
    21001 ggagaacatt cacacaggct gcttgtttct cctttacaag agctcgagca atcgtcatgg
    21061 cttccttccg gctcgacgta gtcacgaaga caagaatgat ctgactggtt gatttcctca
    21121 tacggtggcc gaccttacaa gaacttcctc tttttccgca atggccacca agctatacca
    21181 atcgtgaaat cagcaagtcc catgccctga atagatgtaa aaatgataca ttctcagtat
    21241 tttcaaatac ttgctatcat ttttctggtt atttttcctg caaaatgctc aaccgtcatg
    21301 tgcaattcat atgacagaag ctctaaatat gacaaaaatg aacaaatatt gtcctcaggt
    21361 gaactgcgta tgaactattc aggatcgaaa gtgctcttta aattcattaa ataaattaga
    21421 gtcctcaaat gaatttgaga gactccgctc tctggcggca acattgacag cattttcatc
    21481 gctcacttat tatgcaacat gtggaatgag taattacaag taggagggtg tcatgggatt
    21541 tacgatttcc ccgaaagagc tcaaagccag acttgataag ggagacaaac tcgttcttct
    21601 tgatgtgcgg gaacaatggg aatttgacat ggccaaattg aacggctcga cgctgattcc
    21661 tctgggaacc cttccacagt ccttatccaa gctgagccgg gactcagaaa ttatcgcgat
    21721 ctgccatcac ggaatgagga gcgcggacgc cacgaatttc ctgctccagc aaggctttcc
    21781 aaatgtgaag aatttggtgg gcggcatcga tgcctggtcg gcacaggtgg atgggtcggt
    21841 cccgaggtac tagcccctat taagcaaggg gtgagaattc aagttcaacg gctactaacc
    21901 aagacgttcc ctgaaatact cgactgtcct cttgagacca tcggtcaagt tcattttcgg
    21961 ttcccacgat aattcatggc gcagttttga actatcgatc acactgcgcg cctgctcacc
    22021 tttcttagca ggtccgtgga cttccttgca ggccgaaccg gtatgttgaa ccaggattct
    22081 gaatagatcg ttaatagaag tttccacgcc agttcccaca ttgtatgttc cttgcgtttc
    22141 ctggcccatt atcgcaagat ttgcctccac aacatcctcg acgaacacga aatctcgcgt
    22201 ttgacgccca ttgccgttga tgaccgcctg ctcgttgttc aaaagctttt ggatgaaaat
    22261 cgccacaacc cccgcctctc cctccggatc ttgacgtgga ccatacacgt tcgcataccg
    22321 aagactcaca acttggagcc cactgattcg ttgataatac gagaggtatt gctctccaca
    22381 aagtttgctc aatccgtacg gcgagagagg attggtgacg tgggattccg gcgccggata
    22441 catctcttgc tcaccataaa tggccccgcc ggacgaagaa aagacgactt tccgcactcc
    22501 atgtcgtacc gcctgttgta agacattcaa tgtcccaaga atattgacct gcgcgtcgaa
    22561 catcggatct tctacggact tgcgaacgtc catctgtgcc gccagatgca taaccacgtt
    22621 cggccgttcg ttccggaaca cccgttccaa acgccaactt tgaatatcca atttataaaa
    22681 ccttgcggac cggttgatat tccgacgctt tccggtagcc agattatcca cgatgatgac
    22741 ttcatgtcct tcctggacaa ggcgatcgac caaatgagag ccaatgaacc ccgctccgcc
    22801 ggtgaccaat actttcatgc atccctccat cgcatacgaa gcgcccatca agaagggttg
    22861 attataccct gacgcctaaa gctcacaagg atttctcgat tcccttttct ccccttcacg
    22921 ggcgaatcga gcacgccctg tacctgaagc cccagccgag caccgcaatc gatgaccttt
    22981 gtcgtcaccg cctcgcgctg cgcatcatca cggacgatgc ctccggttcc cacttgtcct
    23041 ttccccacct caaactgcgg cttcaccaga accacaaggg tggcatcccc acgaaggagt
    23101 ggtaccacgc tgggcataac gagcgtgaga gagatgaagg acacatcgat gaccgcgagt
    23161 tcgatcggct caggaatcaa cgtcctgtcg acatgtcgaa tgttcgttcg ttcgagcagc
    23221 acgactcggg gatcctgacg cagtttccag tcgatttgcc cgtacccaac gtcaacggcg
    23281 taaatgcgct tcgcgccgcg ttgcaggaga caatccgtaa acccgccagt tgaacatccg
    23341 acatcaagtc caatcacgcc attgggatca atcgagaagg cctcgagtgc ggcagccaac
    23401 ttctcaccgg aacgactcac atacggtgcg ggcttttcga tttcgatccg tgcatccaag
    23461 ggaacgagtt tggccggctt gtcgacaata gctccgtcga caatgacacc acccgccagg
    23521 atcgtcctcg cagctacctc ccgacttgct accaagcctt gagtgacaag cagacggtcc
    23581 aatcgttctt tcgagatgcg caccctcgag tccatgaaga tgagacggga cgagtatggg
    23641 agaacggacg ttgggagccc tctaccattt cgcactaaga agtagattcg tccgcttcgt
    23701 cgtccggtga aaaggcgtga agccgcttct tgccgttctt gtcttggacc agcacctcaa
    23761 ccttgtgctc ggcgtcctca agaatcttca ggcaactttt tgacagacgg atgccttctt
    23821 cgaaaatctt aagagattca tcgagcggaa ggtcgccctt ttccagttca cctacgatgg
    23881 cttccaaacg agccatagct tgttcaaatt tcactccgcc cacaatacct ctcttccttc
    23941 cccatccctg tgcgccccat tatacccagc acgatccgga tttcaaaccg atggatcggg
    24001 cagcacacgc ttgactgtac agttcagctc tccttgagac agtcgcgcaa tgatctcctc
    24061 cccaacgcgc acgtcctgcg atcgtcgaag aatagccccg tcccgaaccc tcgatagaat
    24121 actgtacccg cgtccgagga ttgccaacgg gctcaagttg tgcagttgag ttaccgtcgt
    24181 ttcgatctgg cgccgtcgac gttcgcttaa tacagccatc tgccgctcca atcgcttaac
    24241 aaattgcggc atcatggcca aacctcgctt gaccaaaaca attggattca gcccggctag
    24301 tccctgctgt ccgtctcgca cctgctctcg agcttcagca agctggagcc gcattttacg
    24361 ctgcaagcga tcggccaact catctgtccg ctgagactct gcctgaatcc ggaacctgat
    24421 ctctgcgagt ccccgcatca tcctgtcgag ccgccgatat tcaaacaggc aatgccgggc
    24481 catcgcccgc cctccacgaa cggctaattc gcgcagccgt tcgaccactt cggcaagaac
    24541 aggaacgacc gtttcagccg ccgccgatgg cgtaggcgca cgaacatctg ccgcaaaatc
    24601 ggccagcgtg acgtcgatct catgtccgac agccgctaca accgggatgc gagagttggc
    24661 aatcgcgcgg acaaccactt cttcattgaa gctccagaga tcttccagcg accccccacc
    24721 tcgaccaaca ataatcacct cgacagcacc ctgggcattc aacacatcca gggcctcggc
    24781 tatttgttcc cccgctccct ctccttgcac ctgtaccggc gcgatgatga tatgcaatgt
    24841 cggccaccgt cgccggaata cggcgaggat atccctgatc gcggccccgc tcaacgacgt
    24901 aacgaccccg accgttcgcg ggaacgccgg tagcggtttc ttcctgctct cgtcgaacag
    24961 tccttccgcc gcgagtcgtt ctttaagttg ttcgaacgcc agttggagtg caccgattcc
    25021 cttcggctcc accgcttcga gtacgatttg gtattcgccg cgcggctcat acacggttaa
    25081 ccgcccccgc acgataacct gcatcccctc ctgcaaagaa aatcgcaccc gcatcgcact
    25141 cgaacggaat aacactgcgc gaatctggct cgattcgtcc ttgagcgtac agtagacatg
    25201 cccagatcca ggcaagcgca ggttcgaaat ttccccctcc aaccacaggt cgggaaactc
    25261 cgattcgatc gacgcgcgaa ccaaactggt gagttcagat acggtgagaa tctgtttagg
    25321 aaagcctgat acagtctgac gttttgtcga agtcatccct tagtcgagaa tgcggatacg
    25381 ttcgatagac aaggcttttc cgatccgcgc atcgagttcg atcaatgcgg cgcagaagac
    25441 ggtcggcccg gaggccactt cgaaacgacg cggcatgccg gtgaggaatt tttcgatcgc
    25501 cagctctttt ttgacgccga tcacaccgtg cagaggaccg gtcatcccga tgtcggtgag
    25561 gtaggctgtg cctcttggaa gaatttgctc gtcggccgtt tgcacatgcg tatgggttcc
    25621 aaccacggcc gtcgcttctc catcaagata gtggcccatg gccattttct ccgacgtggc
    25681 ttcggcatgc atgtccacca gcacggcggc cacgcgcttc ctgagtttcg ccatctcgcg
    25741 cttcgccact tgaaatggac agtccagcgt cggcatgtac gcgcgcccca tgagctgaag
    25801 aatacctaat tcttcgccgc cgggcgtctg aacgaccaca ctgccctgcc ccggcacacc
    25861 ctctggatag ttggcaggtc gaagcaagcg gcgctctcgc gggaagtagt cgagaatctc
    25921 cttcttatcc catgcatgat ttccggttgt aatggccgag agccccagac cgaacaactc
    25981 ttcagccagt tccggcgtaa tcccgaatcc tcccgcaaca ttttccccgt tcccgacaac
    26041 cacatcgatc ttgcgctgcg ataccagacg cgggatggcg cgggcaaccg cccggcgccc
    26101 aggttcgccc ataatatctc cgatgtagag cactttcatt tcgcgtactc gacgtagcgg
    26161 ctctcacgga tcacggttac tttgatctgt cctggatagg tcaattcctg ctcgattttc
    26221 ttcgccaagt ctcgagagag ctggaacgat tcggtgtccg tgacgtcttc ctgtcgaacg
    26281 atcactcgaa tttcccggcc tgcctgaatc gcgtaagcct tctggacgcc cttttgggca
    26341 tgggcgagcg attccaactt ctccagccgt ttgacatatg actcaagcgc ttcgcgtctg
    26401 gcgcccggtc gtgccgcaga caacgcctcg gccgccgcca ccaatactga ttccggacag
    26461 atgggttcta cctgttcatg atgagcggcg atcgcattga cgatcttctc actttcaccg
    26521 tacttcttgg caatctcagc ccccagcatg gcatgcggtc cttcttcttc atggctcacg
    26581 gctttgccga tgtcatgcaa caatgctccc ctcctggcca atcgaacatc gagaccgagc
    26641 tccgaagcca tgattccaca aatgtaagct gcttccctgg catggtagag attgttttgg
    26701 ccatagctgg tgcggtactt gagccgtccc agcactttga tcaattcggg atggaaatcc
    26761 gcaagcccca gctcgaaaat aatcttttcc gcttcttcat acatcaattt gtcgatgtcg
    26821 accttcacct tctcgacgat ctcttcgatc ctggtcggat ggatgcgccc atcgtgcatc
    26881 aaccgttcga gcgagacctt cgcaatttct cgccgcagcg gatcgaatcc ggagataatc
    26941 acggcttccg gtgtctcatc gataatcaaa tcgattccgg tggcggcttc gatcgcccgg
    27001 atattccgcc cttcccgacc gatgatgcgc cctttcatgg cgtcgttggg aatcgggacc
    27061 actgaaatcg tagattccga gacgtaatcg cgaaccaccc gttgaatgga agtggtaatg
    27121 atctcccgcg cctcccgttc ggcattctcc ctggcctctt ccaaggtccg cttggcgatg
    27181 cccgcggcgt ccaagcgcgc ctggctttcc atttccaaaa gcagatgtcg cttggcttcg
    27241 tctgccgtca tccccgccac acgctccagt gcctcacgat gctctttttc agcttgcgca
    27301 caagccactt cctttgccgt aagccgctct tcccgtttgc ttaattcctg ttcgcgtctc
    27361 tgcccatcgc tatcgcgttt atccaaaacg ctgatccgct tatcgatggc ttcttctcgt
    27421 tggacgaggc gcttttcagt ggtagagagt tctgctaatt tggctttctg ctccttctca
    27481 agatccgcct tggattgaaa gacaaggtcc ttggcctcaa gcttcgcctc cttgacgata
    27541 ttttccgctt cgcgttgcgc gttttggatg agatggcgag actgctcctc ctcttgggcc
    27601 ctcttggccc cagccgtcga tcgacgaacg acttcaaaca accccactcc gagcaaggca
    27661 cccacaattc caattaacag atagacaaca attgaggtcg acattggact gacccctttc
    27721 acaatcgagg ccgaggagtc ctcctctcag cctctaccag gaaaacattc aagacggttg
    27781 tgtggggaat gcagcgggaa agatgaatcg acggaagagg acgatcgatc tacgattggt
    27841 cgactactcc gagaaacaaa cccgggagga tgaacgtgtt gctggattga cagatcgagc
    27901 ctgtcccctt ccagtgaaat ccctctccgc tcgatacgaa cgggccgcgc ctcgggttgg
    27961 cacatgcacc gaacgcactg ggaggaggag ccccggtctg cctgctccag acgtaggtcc
    28021 ggtgggcagc acccacccca ccaatttacc cagcatggct aagagggttg atagaagaag
    28081 atcggcccct tgcacaaatt tccactctct taaaggcacc agcatcgaca atcccacatc
    28141 cattcctgca tcaacgaaat cgaatcccgg attttcgacc ggttcgcgat tgacttgatc
    28201 ctaatcccta tcgtcgtaaa accgcttccc aacacacaat tctagcgttt acgataacaa
    28261 acgccctacg ccattgcaag cgcaattatc gaaatagcga agtcggaacc tgctcctcga
    28321 tcgactccat gagcgacgtc atacgccgtt ctacatccgc ctcaccctgt aatcgcttct
    28381 tttccgactc gaacaattga tgagccaggt tcagagccgt cagcacagcc agcttggatg
    28441 gcgtcgtcgt gttcatccct tccgccagct gacgcatctg cccatccact agccccgcca
    28501 gcctgcggat ataggcttca tccgcctcgc ctcgaatcgc ataccgttgg ccgtagatct
    28561 cgacatctgt cgtcttagtc aaaggccacc tccttgcgtt cttcaaagca ctccaatagc
    28621 tcgatgtctc caagcacctt ttcaatgcgc accctgatat ctccccgttc ccgttcccaa
    28681 cgccggtttg agtcgctttc ttctgcaaac cgctgacgag ctgccttgag atcgtcctca
    28741 agggcggcat ttttcttctt caactcatgt acaagcttga ccaagtcctt aatacgggtt
    28801 tccagcgcgt ccaaccgatc caatgccatt ctattatgct cctcctcagc ccctacagat
    28861 tgtggggcac tataggaact tgcccacatc ttgtcaagaa aggcagggct aggaatgtcc
    28921 tcccaagcgg acatattccc ggtagcttcg caagacgcgt ttcacataga gccgggtttc
    28981 ttgataggga attagctcga cgaattcgtc ttggctctgg ctgcggtact gcgcgatcca
    29041 gttcccgacc acgatcggac ctgcattgta tgatgcaatt gtatagatga gatttccgga
    29101 aaactgctcg agcaactgtt ccacgtagcg gacaccgatg cgaatattcg tttcccggtc
    29161 gaataaatcc tcccgcccca ccgcagggag gcccacccgt tgagccacat tgttcgcggt
    29221 tgccggcatg acctgcatga gcccgatggc gcctacgcgc gagaccgcct tctcatcata
    29281 ctgactttct tctcgaataa tggcggcaat gaaataagga tcaacgcctt tggccccttg
    29341 cagcttaatc gtcggcaaga gtccggtcgg gtaggccaca ttccataagg acggcgcgat
    29401 atcccctccc gtccgctcca gcttgtcgcg aaaccgtgtc cgcaccatgc gcagggcatg
    29461 atgataggcg cccacctcgt tgagcagggt tgagagtgca atcagtacat ccgggtctcg
    29521 actgtaacga tccgtcaaca cggctacctc acgagccgca tcggaatcca accccagtgt
    29581 cttgagttcg atcgcccggc ggtacgccgt ctgctgttcg atgtcaacgc gggtgataac
    29641 cggctgcacc gcttctccat tcgtcgatgg cttgctactc gtcgaagcag tctccgcggt
    29701 cgtctcgcta atttcctgta tcgtcgcccg ctctcgcgct aactgacaat aataggtgta
    29761 ccggtagcgc tgacaaactt ggcgatagaa ctcctgtgcc tgaggctgtt ggctttgctc
    29821 ggcggcacgg cccaaccaat acaatgcttg cggctcgaat tcactatcgt actgctcaat
    29881 gagtatccgc agtgcctcgg ctgcttcgcg gtaacgaccg gttcggtagt acacccagcc
    29941 gacacgccat tgagcctcag cccgctggga tgccgggtca ccctctttgg caacttggcg
    30001 ataccgcagg agcgcttcgt cgaacttctt ctgatcttcc agccacatac ctgcgaacaa
    30061 caggatctga cccttctgct ctgccgagag gggcgacgat ggcaacgcac ggacgacttc
    30121 cagcaacttg tcaccctgcc cctgtcgaag atagacgcga gccagccaga ccatagcttc
    30181 acgggactcc gatgcaccat ctttggccag agcccggaac gtctcacgcg cctggtcgta
    30241 ttgcttgagc cgaacttgtg caattccgag tttcaacttt gcctcggcac gacgaggaga
    30301 ctgagggtcc gctgcaagaa actgcttgag ttcgtcaatc gcttcaacat ggaacgcttg
    30361 tgaaaggtag gcctgcgccc tgccgaaccg ttccgtaggc tgcactgtcc atggttcccc
    30421 acctaagttg gaggccagca gcgtttccgc ctctttggct tctgacgtat acggaaatcg
    30481 tacccacaaa taccgcaacg tctcgcgccc ctcggtgata ttgttgactc gaagctggca
    30541 ggcgccttgt cgcaaatacg cctgcggggt gcctggttcc ttgtcgctca acgcgaccgc
    30601 cttcgagaac caatttgtcg cctccgggca actcgaggct tgataccatg cctcgccggt
    30661 ccggtacgcg gctctggtca ggatgttcga gtcgggcacc gccgttggaa tgctttccag
    30721 tatcaccgcc gcttgtttcg catcgccaag tttcaaagac gcctccccga tccagagccg
    30781 gatgtagtcg tccaaaaccg gaaagtctcg ctgcgcacca tgtaaaaact gaatcgcgac
    30841 cgcgggattc cgctcgacga gcagcacacc ggatagcaac cccgcccgct tcgcccatac
    30901 ggtggatgga tattgctcca tcagcctttg gagccgttcg attttcaggg aatgcacctg
    30961 ttctttcgtc agggtcttcc ccaaacgttc cttcggcaga gccgctgcct gaaaacaggc
    31021 ctccggggcg ccgcatgtgt caccttcatt cgaagcagat gcagaaggtt ctgccgccgc
    31081 gacaggaaac accaatactc cccacagcca gaaggctgct gcaaggaggg gcatccggca
    31141 ggatagatgg tcaatgtggt tgcacacgag aaacctctcc gtcaccttgt atcatcatat
    31201 atatcagcgt cccccacatg ttgccagcaa gagcggtcac cctcaccttg ttccactttt
    31261 tcccgctgtg ttaggctgct ccatgccgga acgctcttca acaacatcgg cccctatcaa
    31321 tctcctcgct ctggacgaaa aggggctcgc ccggctcgtc aaggagtccg gctggccagc
    31381 ctatcgaacg gggcaaatac tccgttggct ttatcagcgg cgggcgcgga ccatcagcca
    31441 gatgaccgac ctgagccagg ccgaccgcgc caaactggcc agttcggcaa gaatcgggcg
    31501 cgccaccgac tacaccgtct tccgctcctc cgaccagacg agaaaattgg tcctgacact
    31561 cgaggatggg ctcagcgtcg aaaccgtgct gattccggac gaagaccgac tcaccctctg
    31621 cgtgtcaacg caggtgggct gcaccttaga ttgcgggttc tgtctcacag gcacaatggg
    31681 tttgaagcgc aacctgaaac cccatgaaat cgtcgaacag gtcctgaccg ctcaagatga
    31741 actcggtccc gatgaacgaa tcacgaacat ggtcttcatg ggcatgggcg aaccgctggc
    31801 gaatctcgaa gccctgtcgg aagctatccg gcgattgacc aatcagacct ggggacttgg
    31861 ctggtcgcct cggcgcatta ctatctcgac cgccggcctc gcctcgcgat tgaaagacgt
    31921 ggcgccgctc ggtgtaaatc tggcgatttc attaaacgcg acgacggaag atcaacgagc
    31981 cagcttgatg ccggcagtga gcggcatcgc ttcgctcaag acactcgtag cagcctgtcg
    32041 ccgctatccg ctgcccccga cgaggcggct cacgttcgaa tacgttcttc tggccggcgt
    32101 gaatgaccag gcagatgatg cgcgccggct cgtcaaactg gtccgtggca tcgcctgtaa
    32161 gatcaacctg atccctttta acccattccc cggcagtgca ttccaacgtc cctctgatcg
    32221 tgatgtcctc actttccaat cggtcgtgcg acaggccggc attgacgtgt tcatccgaaa
    32281 aagccgcggg cgagatgtgc tcggcgcctg cggccaactc ggcaatttgt ctccgacctc
    32341 cgtagtgcga accttgacac aaattgaatc gcgttgttag catgcattgt cctgtttttc
    32401 accacacgat atgatcaggc cgcacctccg acacatcgca tccaggttac ttctggcaac
    32461 tgtcttcgtg ctcgtatcga tcgctcctgc cggcgcttca gagccccatg aatatattct
    32521 ctcgaacggc atgaaagtgc tgctcgtaga ggttccgaaa gctcccgtcg cgacggtcca
    32581 ggtgtggtac aaagtcggct cgcgcaacga agtcatgggt cgcgcgggac tctctcatat
    32641 gctcgaacac atgatgttca agggcaccgc caagtatccg aaagggtcgt tctcacgcct
    32701 catccgcaag aacggcggaa tggacaacgc cttcaccagc caagacttca ccgcctactt
    32761 tgaaaatctc gctgcggacc gagtcgaact cgccttggaa atggaagccg accgcatgca
    32821 gggactcatt ctggatatga atgaattgaa aaccgagcgc gacgtggtca aggaagaacg
    32881 acggctgcgc acggaagacg atccgcaagg cgcgctggtc gaagcgctgt tcgcccaggc
    32941 ctacctgagt cacccctatc attggcccgt catcggatgg ttcggtgacc ttgatgccat
    33001 gaccctcgac gatctccaac ggcattacga cacgttctac tcgcccaaca atgcgacgct
    33061 ggttgtcgtc ggcgacatca agactgagac catgctcccg acgatcaagc agctgttcga
    33121 gccgattcca cggggaccgg aacccaaacc ggtcatgacg atggaggggg aacaaaaagg
    33181 agagcgccgc ttcctcctca aacgggatgc tcaagtcccc ttcgtcatga tgggctatcg
    33241 tgttcccaac tacaccagtg aagactccta tgcgctggat gtgctggagt cgattctgtc
    33301 gcgcggcaag agctctcgtc tctatcaaag cctcgtctat gaacagaaat tgtctcttgc
    33361 agtgggagcc gagtatggcc tgatgcagac cgatcccgga ctgttctatt tctacgcgct
    33421 cgtgagcccg gggcaaaagg tcgacgtggt cgaggaggca ctgcacaaag aaatcaagcg
    33481 tctccagaac gaaccgccca cggatcaaga actccagcgg gccaagaatc aagttgaagc
    33541 cgcccgggtg ttcgagcagg actcgaattt ccggcacgcg atgctgctgg gacaggccga
    33601 aaccgtggga gcgggatggc ggaaggtcga tcaatttctg gatcggatcc gctccattac
    33661 ggccaaagac gttcaacgag tggcacgtca gtacttgatt gaagacgcga gaaccgtcgg
    33721 caccctcatt ccgcttcctc cgaaacaggc ggacgcgcct accgcaacag cgcaaccagg
    33781 gaagccttga agatgcggat cccccgacag acctcccatc ccttggggcg gctacttgca
    33841 tgtactctgg ctctctatgc catggtcctc gcatcgttca acgctccgac cctcgcagca
    33901 gatattgtcc ccacgaaaac ggtgactccc aacggcatga ccgtcctcgt gctggaacaa
    33961 catttcctgc cgatcgttga agtgcacgcg ttgatcaaaa ccggctccgc gcaagatccc
    34021 cccgataagg ccggcctggc aaaccttgtt gccagcctgc tcgatgaagg aaccaccacg
    34081 cgcacctcca aacaactcgc ggagcagatc gacttcgtag gcggagcact ggaggtcaaa
    34141 gcctcagaag atttcaccac tgcctctgcc cgcgtactca agaaagacgt agatctgggc
    34201 ttcacgctgc tcgccgatat cttgcaacgc ccggcatttc caaaaccgga attcgaacgg
    34261 atccggtcac agattctcgg tgagatggcc agcgacaacg acgatccagg ccatgtcgcc
    34321 atgaaagcct tcaaccaatt ggtttttcac ggccatccct atcgctggcc gttcaacggc
    34381 acagaagaat cccttgccaa gatcactcac gcagatgtgc tgaactttta caacaaggag
    34441 tacgttccgc ctcaggtgat tctgaccatc gtgggcgacg taacggctga gcaagtcacg
    34501 gctttggtcc agacgcattt cggcgcttgg aagaaagccg caccgccggc acgaaccacg
    34561 aagaaagccg ctccggtaga tcgcaagacg gtgcagctga tcgagaagga tctgacccaa
    34621 tcgaacgtga tcctgggaca cggcggcatc agccgcacaa accccgactt ctatgccgtg
    34681 accgtcatga actacatcct cggcgcggga ggattctcct ccaggctgat ggactccatc
    34741 cgtgacaagc agggtctggt gtatggcatc atgagccact tcgatgcccg gctgatgccc
    34801 ggttcttttt gggtcaactt ccagacgcgt actgaagcca caaatcaagc tatccaggga
    34861 gtcttgggag aactcaagag catccgagag gcaccggtgt ccgatcagga actctccgag
    34921 gccaaatcat ttctcatggg cagcttcccg ttgcggctcg actcaactgc caaacttgca
    34981 caggtattgg ctcaggtaga gttttacgga ttgggcttcg aatacttttc ccagtatccg
    35041 aagtggatcg agcgtgtaac gaaggaagac gttcagcgcg tcgccaaaca gtatctcgac
    35101 ccccaacgat atgctctcgt cgttgtcggc aacgtggcca aggccaaaat caaacactga
    35161 gcgaaattcg tctcccgcga gtcctcatat ggcaacggaa caactcactg agaaactgaa
    35221 cgatcttcga cgcgtgatct cggacatgca atccgtcctt gttgcgtttt ccggaggcat
    35281 cgacagcacg ctcgttctga aaatcgccca cgaacaactc ggtgaacggg cgtgcggcgt
    35341 caccgccgtc tcgcctacct tcccgacgat tgaactcgag acagcgaccc gcatcgccgg
    35401 cgagatcggg gcgcgccacg aaatcgtccg cacggaccag ttgaccatac cggcattcgt
    35461 ccagaatgac gcgacccgtt gtttccactg caagaccgat ctgtatcaat tgttggaagg
    35521 catgcggcaa ccacgcgcgc agtgggtcgt cgatggtaca aacctagacg atctgggcga
    35581 cgaccgaccc ggcatcaaag cggcgcgcga atggaacgtg cggagccccc ttgttgaggc
    35641 atcgtgctcg aaggctgatg tgcgggtcat cgcgaagatg ttggatctgt ccagttggga
    35701 taagccggcg gcagcttgtt tgtcttcacg aattccccgc ggcactccca ttacgatcga
    35761 gaaccttcga cggatcgaac aggccgaagc cattctccag accgaaggct ttcgccaggt
    35821 gcgcgtcaga gaccatggtg agatcgcgcg gattgaagtg gcggaagacg aactcgcaag
    35881 attcaaccag ccagaccgac gcgcacggat cagtgcccgc ttgcgcgaag ttggctttcg
    35941 attcatctgc gtggatttgg aaggatacag accgggcggg atcagtctgg gctgacccgc
    36001 ccgacgtttg atcttatctt ggagaagact tgctgagagc ttccagcgcc tcgtcggcga
    36061 gccggcgatg atcggcgtcg ttcaaactgc gctgcactac tttttccgcc accatcagcg
    36121 ccaaatctgt cgtttgcgtg cgaatctcct gaatggcttt ccgtcgttca tgatcaatct
    36181 cgcgggtcgc atcgcctttg atccgctcgg cttccgtcgt gagtcgctgt tcgttttctt
    36241 ccatcaggcg ctgcgcccgt tcttttgcag ccgcgagaat tccttcagct tctttcccaa
    36301 cggcgctgag cttcgcctca tactctttga gcttgcgctc ggcgtctgag cggtgacgct
    36361 ccgcctgatc aaggctgtcc ttaatctttt tctcccgttc ttccagcata ctcaagatgc
    36421 cagggaaggc gaacttgtag agcgcaaaga acagaatggc gaaggagatg acctcccaga
    36481 agatcaacga cgaaaaaaag tgtgaatcaa actgcggcat agttcacatc aggaaggtta
    36541 aggtt
//
LOCUS       DATIFR010000255         6017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1141490, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000255 DATIFR010000000
VERSION     DATIFR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141490"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VLD60_14775"
     CDS             <1..256
                     /locus_tag="VLD60_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSB46276.1"
                     /translation="DHMAQKTGLTKKACVQALDDFAALAYREAKNVFVVPGIGKLVLA
                     NRKARMGRNPQTGEAIKIPAKRVVKFRVSKAAKDAILGKK"
     gene            complement(662..937)
                     /locus_tag="VLD60_14780"
     CDS             complement(662..937)
                     /locus_tag="VLD60_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSB46277.1"
                     /translation="MVTISLMGQLQTADGERDLACEVPVPLTVRQVIQRQGIQLRHLL
                     QLIREKKVLVTINKKIASDDSLVHDGDAIRLVGHDGFGGSGLGPSHS"
     gene            1005..1421
                     /locus_tag="VLD60_14785"
     CDS             1005..1421
                     /locus_tag="VLD60_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46278.1"
                     /translation="MPSLNIHNPGMPDLQFVLFVSALCTSNIETINLSEDLRKTIFDR
                     CWALLHTDAPPVDPKERVLDLRHGTELTLEACVSTIRSLLSEAGITTLVWDHPVSDPT
                     HDSTPAAKPLIDRLGQLYPDNPDIVDPPQPPPAGRA"
     gene            1452..2015
                     /gene="rfbC"
                     /locus_tag="VLD60_14790"
     CDS             1452..2015
                     /gene="rfbC"
                     /locus_tag="VLD60_14790"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSB46279.1"
                     /translation="MRITPLEIPGLLLIEPTLFRDQRGVFCEVFHERRYAEAGFTEQF
                     VQDNYSRSIRGTLRGLHFQEPHAQGKLVMAVEGTVYDVVVDIRKGSPMFGKWYGVELS
                     SENCRQLYIPPGCAHGFCVTSEQAAFLYKCTDYYSPKDERGIIWNDPALGIAWPEVTP
                     ILSAKDQAYRPLAQMDAELPLYRPDKR"
     gene            2131..2559
                     /locus_tag="VLD60_14795"
     CDS             2131..2559
                     /locus_tag="VLD60_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSB46280.1"
                     /translation="MSKSLKGTKSHENLKNAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTDANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFAELAEWFETLAKAERSHANRFQKGLDSLKQG"
     gene            2657..3931
                     /locus_tag="VLD60_14800"
     CDS             2657..3931
                     /locus_tag="VLD60_14800"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSB46281.1"
                     /translation="MKGLSLINPVDPKQLDKETLRIYEICDGCRRCFNLCPSFNTLLD
                     RIDVYAGDVARLTPADHHQVVDECYYCKLCFNHCPYTPPHQYELDFPHLMILWKKRLA
                     GERGVRWRDWLLIRTDLIGTLGSLTAPLTNWLLQNRIVRRLVELIAGVHRDRQVLHFT
                     GETFPRWFSRRPAPTRATVPVKKVALFSSCLVNYQATDIGKATVQVLEKNGVEVVVPE
                     QRCCGMPGFDIGDTQAIQEAAQANVASLQRWVAQGYDVVVPTASCSLMLKHEYPELNN
                     DEHTRQVAERTFDICEYLMKMKKDGQLAMDFTRSPGRVAYQIPCHLRDQNIGFKSKEL
                     MECAGAQVELIEKCSGHDGAWSAKTEFFSLSMKIAGKAVRAIERSPADLVASDCPLAG
                     LQLDQAGATAHVGGKATLHPIQIVRDAYGLPK"
     gene            3928..4509
                     /locus_tag="VLD60_14805"
     CDS             3928..4509
                     /locus_tag="VLD60_14805"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSB46282.1"
                     /translation="MKLLTQQDVLSQEEYERQREAFRSRIIALKQRRRISVGPLITLI
                     FENRETLRFQVQEMIRVERIVEPAKVQDELDVYNALLPSPQELSATLLIEITEEARIQ
                     EWLDAFMGLDRGQTVAIEAGGERAFGEFEGGHSHETKISAVHFVRFRPTAAMKEAFAD
                     LRQPVTISVNHGSYHEEVPVPGSMREEWLADLQ"
     gene            4559..5308
                     /locus_tag="VLD60_14810"
     CDS             4559..5308
                     /locus_tag="VLD60_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSB46283.1"
                     /translation="MPPVLLTKRIEFAAAHRYIKPEGDEAKNRAVFGPCYNPPAHGHN
                     YMLEVTVSGEVDRKTGMVINLFDLKRVLLTVIEEFDHKNFNLDMSYFNDRIPTSENIA
                     RLLWTKLEPQQDIGTLHTVRLYEDEDLYADITAAGGLDVASVTRRYSFMAVQEGNQGH
                     VWDCFVTVHGPIDCVTGMVTDIGALDRLAQERVVKAFDRQVLERVLGQPVTGDALARH
                     VWGRLAGSVNGGALELVRLVQTRDLSFEYSG"
     gene            5353..5832
                     /locus_tag="VLD60_14815"
     CDS             5353..5832
                     /locus_tag="VLD60_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HSB46284.1"
                     /translation="MTRPAISVLMITLQVFAGSVVLAGQDEDAVQALLIGAAKATSEF
                     SRTRDPQSVLKAYTKDYVGIQDGESETRGTIEKWLSEYGAELDKGNPSRFLGEIFGIK
                     VRVSGTLAWATYDYVFKLVANGEIQTEDRGLCTSILRKEGSAWLIQHEHCSKPRPAK"
BASE COUNT         1288 a         1685 c         1784 g         1260 t
ORIGIN      
        1 cgatcacatg gcccagaaga ctgggctgac caaaaaggcg tgcgtccagg cgctggacga
       61 cttcgccgcg ttggcctatc gggaagcgaa gaacgtgttc gtggttcccg gcattggtaa
      121 gctcgtgctg gccaatcgaa aggctcgcat gggccggaac ccgcagacgg gcgaagcgat
      181 caagattccg gccaagcgcg tggtcaagtt ccgcgtctcc aaggccgcca aggatgcgat
      241 cctcggcaag aagtagtccg ggttcagaaa acagaccgta gcgccaaggc tgccaaaacg
      301 ctttccgcta tcggttgaga gagacgaagg gccggctccc catagggggc gccggccctt
      361 ctctttagca gactgttgaa aaagtattcc agctgcattc tcgcgtcgct cagaggctca
      421 gggcatacag aagccgactt gaggcaaagt tacgatacgc tcacatgtga ttgaagtgag
      481 cgactcaagc gaagcgtggt atgtacctcc ttgcccctgt gcttgctgcg gccttgcctg
      541 cggaacggtg cgtctcggtg caccggggtt gggctggtga gaatcgaggc cttttgaaca
      601 actgcggtca gcgagcacat ctggtcgatt gcctcgacct tagcctcgtt ctcagcgcgt
      661 tttacgagtg cgaaggcccg agcccgcttc cgccgaatcc atcatggccg acaaggcgga
      721 tggcgtcgcc gtcatgcacc agcgagtcgt cgctggcgat ctttttgttg atcgtcacca
      781 gcaccttctt ctctcggatc agttgaagga gatggcgcaa ctggatgccc tgccgttgga
      841 tgacttgccg gaccgtcagc ggaaccggca cttcgcaggc aagatcccgt tcgccatcgg
      901 ccgtctgcag ttgtcccatc agtgaaatcg tcaccatgtc gtgatccgct ccttccggtc
      961 aatcccgttg actccgcgga ggatgccatc gataatgccc cgccatgccg tcgttgaata
     1021 tccacaatcc tggaatgcct gacttgcagt ttgtcctctt tgtgtcggcg ctctgtacct
     1081 cgaatatcga gacgatcaat ctatccgaag acctccgcaa aacgatcttc gatcgctgct
     1141 gggccttgct tcacacggat gcgccgccgg tcgatccgaa agaacgagtc ctcgatcttc
     1201 gtcatggcac cgagcttacg ctggaggcct gtgtgtcgac catccggtcg ctgctctcgg
     1261 aggccggtat tacgacgctt gtctgggacc atccggtcag cgacccgacg catgacagca
     1321 cgccggctgc caagccattg atcgaccggc tgggccaatt gtatcctgac aatccggaca
     1381 tcgtcgaccc cccgcaacca ccgccggcag gccgtgccta gacgtgccgg gcgtgaaggc
     1441 cacagacaac catgcgtatc acaccactcg agatccccgg cttgctgctg atcgaaccga
     1501 cactctttcg cgaccagcgc ggggtgttct gtgaagtctt tcacgagcgg cgctatgcgg
     1561 aagcgggttt caccgagcag ttcgtccagg ataactattc acggtcgatc cgcggcacct
     1621 tgcgcgggct gcactttcaa gagccccatg cccagggaaa gctcgtcatg gctgtcgagg
     1681 ggaccgtcta cgatgtcgtg gtcgacatcc gcaagggatc gcccatgttc gggaagtggt
     1741 atggagtcga actgtcttcc gagaattgtc gacagctcta tattccaccc ggttgcgcgc
     1801 atggtttctg tgtgacgagc gagcaggcgg catttctcta caaatgcacc gattattatt
     1861 cgccgaagga cgaacgcggc atcatctgga acgatccggc gttgggaatt gcctggccgg
     1921 aggtgacgcc gattctatca gctaaagatc aggcatatcg gccgttggcc caaatggatg
     1981 cagaattgcc cttgtatcga ccggataagc ggtaaggata gtcgttgatc ctgtcaggat
     2041 ggctgggcta tcttatagcc gggtagtcgg ttgaaacggc acaggccgct caaccatcag
     2101 gccggtatcc ctacgtacaa ggaggatcga atgtcaaaga gcttgaaggg gacaaagagt
     2161 cacgaaaatt tgaaaaacgc ctttgctggt gaatcacagg ccaatcgccg ctacctgtac
     2221 ttcgcccggc gtgccgatat tgaggggtat cccgatgtgg gcggcctgtt ccgggacacg
     2281 tccgaagccg agacggggca cgcgttcggt catctggact tcttgaaaga agtcggcgat
     2341 cccgccacgg gcgtacccat cggcaatacg gacgccaatc tcaaatctgc gatcgagggc
     2401 gagacctacg agtacacaca gatgtatccg ggaatggcca aaacggctcg cgacgagggc
     2461 ttcgccgaac tggccgaatg gttcgagacg ctggccaaag ccgaacgttc ccatgcaaac
     2521 cgattccaga agggactcga cagtttgaaa cagggctaga aagaagcctt cagcgttcag
     2581 ccgtcggctt tcagcacgga gaggcctctc ttcggagctg agagctgatt gctgatagct
     2641 tccctcctgc atccacatga agggcctcag tctcattaat ccggtcgatc cgaagcagct
     2701 ggacaaagaa acgctgcgga tctatgaaat ctgcgacggc tgccgccgct gtttcaacct
     2761 gtgcccgtcg ttcaataccc tcctcgatcg tatcgacgtc tatgcaggtg atgtcgccag
     2821 gctcacgcct gccgaccatc atcaagtggt cgatgagtgc tactactgca agctctgttt
     2881 caaccattgc ccctatacgc caccgcacca atacgaactc gactttcctc atctcatgat
     2941 cctctggaag aagcgattgg cgggggagcg cggggtccgt tggcgggatt ggctcctcat
     3001 cagaacggat ttgatcggga cgttgggcag cctcacggcg cctctcacca attggttatt
     3061 gcagaatagg atcgttcgcc ggctcgttga gttgatcgcc ggcgttcatc gggaccgcca
     3121 ggtactgcat tttacgggcg agacctttcc tcgttggttc agccgccgcc cggcacccac
     3181 acgcgcgacg gttcccgtga agaaggtggc gctcttttcc agttgcctcg tcaattacca
     3241 ggccaccgac atcgggaagg cgaccgttca ggtactggaa aagaacggag tcgaggtggt
     3301 tgtgccggag cagcgatgct gcgggatgcc cggtttcgat atcggcgata cgcaagccat
     3361 tcaagaggcc gcgcaagcca atgtggcctc gctgcaacgc tgggttgcgc aggggtatga
     3421 tgtggtcgtg cctaccgcga gctgcagcct gatgctcaaa cacgagtacc cggaattgaa
     3481 caacgatgag cataccaggc aagtggccga gcgaacgttc gatatctgcg aatacctgat
     3541 gaaaatgaag aaggacggac aactggccat ggactttacg cggagtcccg gccgcgtggc
     3601 ctatcagatc ccctgccact tgcgtgacca gaacatcggc ttcaagtcga aggaactgat
     3661 ggaatgtgcg ggcgcgcaag ttgaactcat cgagaaatgc tccgggcatg acggggcctg
     3721 gtccgccaaa acagagtttt tctccctgtc gatgaagatt gcgggaaagg ccgtccgggc
     3781 gattgaacgg tcgcccgccg atcttgtcgc atccgactgc ccgttggctg ggttgcagtt
     3841 ggatcaagcc ggggcgacgg cccatgttgg aggcaaggcg acactccatc caatccagat
     3901 cgtccgcgac gcctacgggt tgccgaaatg aagttattga cgcaacagga tgtcctctct
     3961 caggaagaat acgagcggca gcgggaagcc ttccggtcac ggatcattgc gctcaaacag
     4021 cgccgccgaa tttccgtcgg cccgctgatc acgctgatat ttgagaatcg tgagaccttg
     4081 cgctttcaag ttcaggagat gattcgcgtc gagcggattg tcgaaccggc caaggtgcag
     4141 gatgaactgg atgtctacaa tgccttgttg ccctcaccgc aggaactcag tgccaccctc
     4201 ttgatcgaga tcaccgagga ggccaggata caagagtggc tcgatgcctt catgggcctc
     4261 gatcggggac agacggtcgc cattgaggcc ggaggtgaac gggccttcgg ggagttcgaa
     4321 ggcggccata gccatgagac taagattagc gccgtgcatt tcgttcgttt ccggccgacg
     4381 gcggcgatga aagaggcttt tgccgatctc cgccagccgg tgacgatcag cgtgaaccat
     4441 ggcagctatc atgaagaagt gcctgtgccg gggagtatgc gcgaagaatg gctggctgac
     4501 ttacaatagt cttgggtcct gagaatggag tgttgagtgt aggagcgaga agaggccgat
     4561 gccccctgta ttactgacaa aacgtattga gtttgccgcg gctcaccgct acatcaagcc
     4621 ggaaggggat gaggcgaaga accgcgcggt cttcgggccc tgctacaacc cgcccgcgca
     4681 tggccacaat tacatgctcg aagtgaccgt gtccggtgag gtcgatcgca agaccggcat
     4741 ggtgatcaat ttgttcgatc tgaagagggt gctgctcact gtcatcgaag agttcgacca
     4801 caagaatttc aatctggaca tgtcgtattt caacgatcgc atcccgacgt cggaaaatat
     4861 cgcccgcctg ctctggacca agcttgagcc gcaacaagac atcggcactc tccataccgt
     4921 tcgactgtac gaagacgagg acctgtatgc cgatatcacg gcggccggtg ggttggatgt
     4981 cgccagcgtg acgaggcgct attcgtttat ggcggtgcag gagggtaatc agggacatgt
     5041 ctgggattgt ttcgtcaccg ttcatggtcc gatcgattgc gtcaccggta tggtcacgga
     5101 tatcggcgca ttagaccggc tcgcgcaaga gcgagtcgtg aaggccttcg accggcaggt
     5161 acttgagcgg gtgctcggcc agccagtgac gggggatgcc ttggccaggc atgtatgggg
     5221 ccggctggcc ggatccgtca atgggggtgc ccttgagctg gttcggctcg ttcagacccg
     5281 cgatctttcg tttgagtact ccggttaatt aaatcggcgg caattattgt ggacgagaaa
     5341 ggactatgac cgatgacacg tccggctatc tcggtcttga tgattacgct gcaagtcttt
     5401 gccggatcgg tcgtcttggc cggtcaggac gaagatgccg tgcaagcgtt gctcatcggt
     5461 gccgccaaag ccaccagcga attttcccgc acgagagacc cgcaatcggt gctgaaggcc
     5521 tacacgaaag actacgttgg gattcaggat ggagaatcgg aaaccagagg gacgatagaa
     5581 aagtggttgt ccgagtacgg tgctgaactt gacaaaggga atcccagtcg ctttctcggg
     5641 gaaatcttcg ggattaaagt tcgggtctcc ggcaccttgg cctgggcgac ctacgactac
     5701 gtgttcaagc tcgtcgcgaa cggcgagatt cagaccgaag accgcggcct ctgcaccagc
     5761 atcctccgca aagaaggttc cgcctggctt atccagcacg agcactgctc aaaaccacga
     5821 ccggccaaat agcttcctgc ttctgaattg aaacttttga gtaagcgcgt gccctctcgt
     5881 tccctcgcct cctgagtaac tctcacgggc ccggcatcga tagattttct ctgtgatggc
     5941 aagggcattg cggaaagtgc actagccggt gcacaaccct tctgtaaacc tgactggcag
     6001 gcgattactt gagtagg
//
LOCUS       DATIFR010000256         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_759146, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000256 DATIFR010000000
VERSION     DATIFR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759146"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1297)
                     /locus_tag="VLD60_14820"
     CDS             complement(179..1297)
                     /locus_tag="VLD60_14820"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSB46285.1"
                     /translation="MATNPASVAALTGAALFVLLATVEPLPTQVRAERAPAVPLGLPP
                     LPMQAHDSSAAAPLGKKLFFERRLSINETMSCGMCHIEAQAFTANEIATSVGMEGKSL
                     RRNAPSLFNVVYEKQLFRDGRENSLETQAWSPILANDEIAAPSVGWALAKLRTLPDYP
                     TLFEETFPGRGITMDTVGAAIAAYERTLLSGNSRFDRWRFGEEADALSANERHGFELF
                     TGKAGCSACHQLGSDSALFTDGKFHDTGLGYAKTMGLEPESFDVRLAAGDFTRQHRSD
                     VESITSPTANDLGRFEVTRDPKDRWAFKTPGLRNIARTGPYMHDGSLGSLKDVVDYYN
                     RGGDFSPNKSELIRPLHLSEEERRDLVAFLESLTGAQQ"
     gene            complement(1287..1976)
                     /locus_tag="VLD60_14825"
     CDS             complement(1287..1976)
                     /locus_tag="VLD60_14825"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSB46286.1"
                     /translation="MVRTLRSIALRIFLPAALFTGAAGSLLAGSVGGLPLPAPGTYKL
                     DRIQEAPFSIVREGTASPSLLSTYTTGKITLLAFFYAHCRDPQGCPLAWNAFETVREK
                     IKANASLQGQVRLVFFSFDRVHDVPETLQLFVDAHKDDAKIVPWHFITAWTDYFLEQT
                     LRNFGQEISVRTDPDGEKSVVIEHLLKVFLIDRDGWVREIYTTGFLDPDAIVGDMETL
                     LLEESQNNLGN"
     gene            complement(1997..3052)
                     /locus_tag="VLD60_14830"
     CDS             complement(1997..3052)
                     /locus_tag="VLD60_14830"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HSB46287.1"
                     /translation="MGFTDDRKYLWAGRLDDSKIFIFDVGTDPAKPTLVNTISNFGEE
                     TGFVGPHTFYALPGRMLVGALSNTKDHGGQTGLAIYSNAGKFLSAHPMPTTDGGDGYG
                     YDIGINPQKNALLTSSFTGWNNYMMDLGKLIKDEAAMKNFGKTMVMWNLKSLKPDTIF
                     DVPGAPLEIRWSLNTKEGDDWAVTAAALTSKLWLINRSADGKWQAKAVADIGDPAKIP
                     LPVDISIAADGKSLWVNTFMDGTTRLFDLSNPEKPVQVYEKKTGSQVNMVSQSWDGKR
                     VYITSSLLANWDKKGADDEQFLKAYDWDGKELVEKFEVDFYKEKLGRAHHMKFTGGQL
                     TAADYPAGTTDPAESTQ"
BASE COUNT          638 a          883 c          855 g          688 t
ORIGIN      
        1 gggatgccct ccctcagtcc ctgctggggt gagataccag cgaatccttc ctgattctag
       61 ctgttcgctc cgcgcgttcc gccgatttct cccgtgccac cggtgagctt agcggcgagc
      121 aatcggcctt ggccgcgagc aatctgccct gagaggagaa gcctgctgac gaggttcatc
      181 actgctgtgc tccggtcagg gactccagga aggccaccag atctcgtctc tcttcttcgc
      241 tcaggtgaag tggcctgatg agttcgctct tgttcgggct gaagtcaccg cctctgttgt
      301 agtagtcgac gacatccttg agactgccca acgatccatc gtgcatgtag gggccagttc
      361 gcgcgatatt tcgtaaaccg ggcgtcttga atgcccaacg atctttggga tcccgcgtga
      421 cctcgaacct gccgaggtcg ttggcagtcg gactagtaat cgattccacg tcactccgat
      481 gctgccgggt gaaatcacct gccgccaatc gaacgtcgaa gctctccggc tctaacccca
      541 ttgtcttcgc ataaccaagg ccggtgtcgt gaaattttcc atctgtgaac aaggccgagt
      601 ctgatccgag ctgatgacag gcagaacagc cagctttgcc ggtgaacaac tcgaagccgt
      661 gccgttcatt tgccgacaac gcgtcagctt cctcaccgaa gcgccatctg tcgaaacgtg
      721 agttgccgga aagcagggta cgctcgtagg ccgcaatcgc cgcgccgacc gtgtccatgg
      781 tgatgccgcg ccctggaaat gtctcttcaa acagggttgg atagtcaggc agtgtgcgaa
      841 gctttgccag tgcccaccct accgaaggcg cggcgatctc atcattcgct aggatcggag
      901 accaggcttg cgtttccagg gagttttctc tcccatcacg aaacaactgc ttttcataaa
      961 cgacgttgaa gaggcttggc gcatttcgcc taaggctctt gccttccatt ccgaccgacg
     1021 tggcaatctc gttggcagtg aaggcttgtg cctcgatatg gcacatgccg cacgacatcg
     1081 tttcattgat tgagagtcgt cgctcgaaga agagcttttt gccgagcggc gccgccgcag
     1141 aagaatcatg tgcctgcatt ggcagtggcg gaaggccaag cggaaccgcg ggggctctct
     1201 cggcgcgaac ctgggtcggg agaggctcga cggtcgccaa caaaacgaac aatgccgcac
     1261 ccgttagagc ggcgacggaa gccggattag ttgccaagat tgttttggct ctcttcaagc
     1321 agaagcgtct ccatgtcgcc aactatggca tccgggtcga gaaaacctgt cgtgtaaatc
     1381 tctcgcaccc agccgtctcg atcgataagg aacaccttca gaagatgctc gatgacgaca
     1441 ctcttctcac catccgggtc tgtccgaacg gatatttctt gtccgaaatt tcgtagcgtt
     1501 tgctctaaaa aatagtccgt ccatgcggta atgaaatgcc agggcacaat cttcgcatcg
     1561 tccttatggg catccacaaa gagctgcagg gtttcaggga cgtcgtgtac gcggtcaaag
     1621 ctaaagaaca ccagtcgtac ttgaccttgc aggctggcat tagccttgat cttctcgcga
     1681 acggtttcga aagcgttcca cgccaagggg cagccctgcg gatccctgca atgagcatag
     1741 aagaacgcca agagcgttat ttttcccgtc gtgtaggtcg agagcaggct tggtgaggca
     1801 gtcccttctc gaacgatact gaagggagct tcctggatcc gatccagctt gtacgttccc
     1861 ggcgccggaa gtggcaaccc gccaacactg ccagcgagca agctgcctgc cgctccggtg
     1921 aacagagcgg caggcaggaa gatccgtagc gcgatagatc gtagggttcg caccacaaac
     1981 accagcttca atggccctac tgggtggact cagcaggatc ggtagtgccg gccggataat
     2041 ctgcagccgt gagctgccca ccggtgaact tcatatggtg ggcgcgaccg agcttctcct
     2101 tgtaaaagtc gacctcgaac ttctcgacga gctctttgcc atcccagtca taggccttca
     2161 ggaattgctc gtcatcggcg cccttcttgt cccaattggc gagcaatgac gaggtgatat
     2221 agacgcgctt gccgtcccag ctctgggaaa ccatgttcac ttggctgccg gtctttttct
     2281 catagacctg caccggcttc tccggattgg agaggtcgaa aagacgcgtc gttccatcca
     2341 tgaaggtgtt gacccaaagg ctcttgccgt cggcggcgat cgaaatgtcg accggcagcg
     2401 ggatcttggc aggatctcca atgtccgcga cagctttggc ttgccacttg ccgtcggcac
     2461 tccgattgat cagccacagc tttgaggtca acgcggcggc ggtaacggcc cagtcgtccc
     2521 cttctttcgt attgagcgac cagcgaatct ccagcggcgc accgggcaca tcgaagatgg
     2581 tatccggctt tagcgacttg aggttccaca taaccatcgt cttgccgaag ttcttcatgg
     2641 cggcctcgtc cttgatgagc ttacccaggt ccatcatgta gttgttccaa ccggtaaagc
     2701 tcgaggtcag caaggcgttc ttttgcggat tgatcccgat gtcatagccg taaccatcgc
     2761 cgccatccgt ggtcggcatg ggatgagcgg acaggaactt gcccgcattg ctgtagatgg
     2821 ccaatccggt ttgcccgccg tgatccttcg tattggaaag cgcacctacc agcatgcggc
     2881 caggcaatgc gtaaaacgta tgcggaccga caaaaccggt ttcctcgccg aaattgctga
     2941 ttgtgttgac gagcgtgggt ttggcagggt cggtgccgac atcgaagata aagatcttac
     3001 tgtcgtcgag acgcccggcc caaagatatt tgcgatcgtc ggtgaagccc atgtgatgag
     3061 cctc
//
LOCUS       DATIFR010000257        37024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_567531, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000257 DATIFR010000000
VERSION     DATIFR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 37024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37024
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567531"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1026)
                     /locus_tag="VLD60_14835"
     CDS             complement(373..1026)
                     /locus_tag="VLD60_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46288.1"
                     /translation="MVRDRNREILSLAGLVGFLVVGISPAFAIDVKLSIEEAQKALEA
                     GRAPMDKANTPEEVKKVLQHASMVTRVGADPEKDSCGPSAVLRTKRYRLEAFGRQEAS
                     ESKKRKTEIRMPEEFIKKVVDMPNMEVEVQLCGDDEYFAEGALIELQQGPKKVKAIDI
                     GKAERGRKNEGSGPAYRSRFTAVFAYESFDPNAASVFVINLQDGKEVRINADFSKVK"
     gene            complement(1095..1727)
                     /locus_tag="VLD60_14840"
     CDS             complement(1095..1727)
                     /locus_tag="VLD60_14840"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSB46289.1"
                     /translation="MRVVLASTSPRRRELLALLEIPFEVRSPAFEESVVPGRSADDLV
                     AYFSQGKARSVAKNDPEAIVLGSDTLIELEGAVLGKPVNLAEARAMLQRMAGREHLVH
                     TAVTVTCLARGIEMTQLSTARVQMKRFDAEVHERYLATGDSLGKAGAYSIQGGGGELI
                     ECLNGDFTTVVGLPLRVVATLLEQAGLRIPVDLHVLYTEKPYENWVRFSG"
     gene            complement(1718..2899)
                     /locus_tag="VLD60_14845"
     CDS             complement(1718..2899)
                     /locus_tag="VLD60_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HSB46290.1"
                     /translation="MSFWQSLARPIIGLSPMDGVTDATFRLIVAQQGKPDVTFTEFTH
                     VHDVCRGPEFLLDSLLYHEEERPIVAQLYGKDPELFYQAAHAVCELGFDGLDINMGCP
                     SRNVASSGSGAGLIRTPDLAHAIMRATRQGIQDWAGGQSLEAAGIKPVRAAAVRRLNE
                     RRGSSAQTIRKSIPLSVKTRLGYDEVIVDRWIEHLLAESPTAISLHGRTLQQMYRGEA
                     DWSAIARAAEVIRGSGTLLFGNGDVQSLQEVLRRVRETGVDGVLVGRAALGAPWFFRG
                     SEDVRKAVRTQGADGPEAWSPSLAARFDVLLEHARLCEMQWGRDQFRRMRKHLGWYCK
                     GFPHAAALRASMFRASSVADVEAMLADYRANRIADGEASSEISSSDIADDTASMVSRC
                     G"
     gene            complement(2896..4380)
                     /locus_tag="VLD60_14850"
     CDS             complement(2896..4380)
                     /locus_tag="VLD60_14850"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HSB46291.1"
                     /translation="MARFRPVEQEFTCRAAVIPTCGLGLSVDVYSPDLFELMKRLEEE
                     KCRPGYLEIFRATTTALQRVRTHFSTVPLAYHGEGLWVTQPDFSTQAFIDEEINEVAN
                     QLGILRSPWLNHECATKQMAGYSFGTYLPPLYTSESARVVASNIAMVQEKMDRACRPA
                     QEFGPLFLLEMAPLTYFMAGTMSVPQFFRFVTELTSCGLVLDIGHLWTVYRYTAARRQ
                     GSLDQFIERFLDEFPMERVIEIHVAGLGRHESLSRRRWEDAHPEWIDAHAAPIQAVSW
                     TILEQVLAHPGLVSLRGVALEVDTKPIEHIVEELREARKNFGSMIHQAIAGRSAALAP
                     VPQRSEFTVMHGAVNDIDRSRLQEDYVRYAQIASGQQPPSGPEWQDVVDDPAGLDCYV
                     HDYVPHEILHWGGDVKEMFPDTCRALDQAGVALDGFVTWWFQAARPVDQPYDFFLLKI
                     ERVLAFVAERAPMALPLAEREAETLRLAYAEANNGVQPMMEPAS"
     gene            4627..5337
                     /locus_tag="VLD60_14855"
     CDS             4627..5337
                     /locus_tag="VLD60_14855"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB46292.1"
                     /translation="MMTPGVVQVPGDVSVSEAALLLEREHAPCLLVKETDTIFGLMTP
                     TDIVKKVVAQGLEPHEIEVRAIMTRPVQFIEYDEALEEASSMMLATGASILIVTKKNQ
                     PVGVLSAQDLMLTPPRRQTKIPTVVSVVGEDLAHRQAQNATIVQLSHVGARIEAATRL
                     HSKARVVLAFSLPGQHTPLTIHGKVMTCESPASDDLKQSGKRAVAIEIQFADLSNVDL
                     ARLKAWALQAIPSQSDRG"
     gene            5439..6482
                     /locus_tag="VLD60_14860"
     CDS             5439..6482
                     /locus_tag="VLD60_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSB46293.1"
                     /translation="MKATPSRPRTMPSNDEMLNQIRQLLERPEDDIQAALIKELLASM
                     LRLHDAHLDSLDLKIVNRAVKELRHAFRVFHDYRDRKKISIFGSARTAADDPNYQIAF
                     DFARRIVQEGFMVITGGADGIMRASQEGAGRENSFGVNIMLPFEQGPNTTIADDPKLV
                     TFKYFFTRKLIFQKEANAIALFPGGFGTHDEGFEILTLAQTGKSDPQPIVCLQAPGCD
                     YWDEWYRFVTGQLLDRKLINPEDLNLFKITNSVDDAINEIRTFYRRFHSIRYVGRQLA
                     MRLTMRPTSDQVAAIHEQFSDLLLEGTFELRDALPEELDEPDLKDLPRLVFASNRRSA
                     ARLRQLIDHLNHL"
     gene            6520..7413
                     /locus_tag="VLD60_14865"
     CDS             6520..7413
                     /locus_tag="VLD60_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46294.1"
                     /translation="MVCSASMATSHPQRRFSQPPTVVLYHAECADGFGAAWALWRQFP
                     EARFLPVKHGNPPPSGLKDQRVVIVDFSYARETLETMAEQADDLLILDHHITAEKALT
                     GLPYAYFDLKKSGAVLAWEWAHDRPAPWLLDYIQDKDLWTWALPQSREINAAIASHPF
                     EFQTWNSFRQKDLEQEGRAILRYEHELVSKLAGQAVPVEFQGVTVPSVQSAILTSQIG
                     ERLSADAPFCIIWHDRDGRRYYSMRSREDGTDVGAIAASFGGGGHTHAAGFSVPLGLD
                     GSLPDNPALPRSVIDRRRTSA"
     gene            7426..8130
                     /locus_tag="VLD60_14870"
     CDS             7426..8130
                     /locus_tag="VLD60_14870"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSB46295.1"
                     /translation="MIPLHDDNPTERTPVVTIAFIVACVLVFLYQASLPSQPGEILVF
                     QYGAIPAIVFGRADLPEGIVAIPAYATLITSMFLHGGWMHLIGNMLYLWVFGNNIEDV
                     MGHARYVVFYLTCGILAALSHAVTEPSSTVPMVGASGAISGVLGAYVLLFPRAHVLVL
                     MPGIGMTRVAAGIVLGMWFVMQLLSGGMSIGSKGGGVAFFAHIGGFITGMVLIGLFKR
                     QDVPFFAPPRTRHWNS"
     gene            complement(8152..10008)
                     /locus_tag="VLD60_14875"
     CDS             complement(8152..10008)
                     /locus_tag="VLD60_14875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB46296.1"
                     /translation="MMRNNLTYSDEYLAERPAKPEGPETPVSRARYVILQSLVGVMLA
                     YQLLFGAEPIATRTTSEFIVGGLVLLVGCLLAVPVSVLQAAWFSGTLIAVDTALVTGT
                     IYLSGNARSDLYISYFILMLIAASLRRLSHVIAFSLFLCAGYGVVLYQGVVQSGSISV
                     GQLLGVPVLLVMAIFYGMALETIGVERRQKARLLESIDELRSAERTLQASREQLEIRI
                     KGLKDDLSKANASVQQERSEREGLERQLFEAQKMEAIGRITNGIASEFSHLFSVIGKQ
                     TGVVLSRLKQDDPLYEPVDQIFRSGEQAAALTAQLAMLDIQHRQFRRLLSMRTVIEDL
                     SDVIRGLLPHGINCRISIAEGPLEVEVDREGLEQVLLNLAVNARDAMPRGGQLTIEVA
                     QAQGGVDGTPLSGTSRHLSTVRIQVRDSGSGMTRDTQSRMFEPFFSTKETNVGLGLTA
                     VYGIVKQHGGQVEVRSQPGHGTTVRIFLPAVASGPSNSQSIVPALVAKGEETVLLVEP
                     QEIERKLALSTLQRHRYRVLEASSPVEALMLAQHYTGAVHVTVSGLIMPEISGRELAK
                     RLLQQYPTMKALFVSGYDDEAIISHRINRRYLLRRPYRQTGLVEKVRELIDA"
     gene            complement(10215..11387)
                     /locus_tag="VLD60_14880"
     CDS             complement(10215..11387)
                     /locus_tag="VLD60_14880"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HSB46297.1"
                     /translation="MTPSPIEPGSVLGVLGAGQLGAMFASAAARLGFRVAVWDPDPDA
                     PAHRVATRSFPKPFTDRQTFAQFADMVSVVTYEWENVPSQLCRELEEMKPVRPSSAVL
                     SIIQDRLIQKTFLADRGLPVPRFAAMTHPDGLAEAIRQIGSPALCKTATTGYDGKGQW
                     RIASESDVPEVERLLGAAAQPGMRWIVEAFVPFERELSILVARGADGQSVVYPPVENE
                     HEEGILRATLVPAAVPQAVAEQAERLARTVMERMQGVGLFCLELFQMPGGTLLINEVA
                     PRPHNSGHYTLDACSVSQFEQQVRAICGMPLGEVRLLSPAAMVNLIGDDATTIGTGAE
                     CRALLNLPAAHVHLYGKRTIRPRRKMGHVTFLADQRDTAQGLATRLRQNLISERPR"
     gene            complement(11384..11968)
                     /gene="purE"
                     /locus_tag="VLD60_14885"
     CDS             complement(11384..11968)
                     /gene="purE"
                     /locus_tag="VLD60_14885"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSB46298.1"
                     /translation="MARSRRPTSRRSANRRTASAQPLVGVLGGSKSDFPILEKAVAVL
                     TELGVPNELLVVSAHRTPDRLFAYAELASARGIEVIIAGAGGAAHLPGMLAAKTHLPV
                     IGVPIPTENLRGLDSLLSIVQMPRGIPVATVAIGGADNAGLLAAQILAGRYPAIAARV
                     KSFRKTQTDTVLRSPEAKGSVAGGSTFSPPRRRS"
     gene            12154..13590
                     /locus_tag="VLD60_14890"
     CDS             12154..13590
                     /locus_tag="VLD60_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSB46299.1"
                     /translation="MVGLGQSFRQKVRSLVQHRSGLDEIAIDDIATSSKLHSWEAVQI
                     PERLKFSSRLAIKLVLLFLLIIAFVPWTQTITVTGQLSAYTPYERPQDIEAQITGRIK
                     KWHIFEGVRVKQGDLILELDDYDPSFMSPDLLSLLDQRKKALSDTRKAALGRADQLDK
                     RIKEMQNLVKAAVPSAQARVVEAENKVREAYQKVEAAKIAVATAELNVDRHKQLAEQG
                     LVSQRELEVTIQSALATKADLSGAQANLKAAEQSMKALSFGREQVSAEVLQRLLDAEA
                     ARDASVAEAAKATDQLADISLRMSNAEQRRIASRILAPIDGTVVKMAETGAGETVRPG
                     EKLVRISPASADKAVEMTADGIDAPLLNVGRKVKILFYGIPAIPLPAWPELMAGTYVG
                     VIKVIDQVDDGKGNFRFWVVPDPDEKPWPPQEHVRQGTKVMGWVILNRVPLWYELWRR
                     FNLFPPDYQERPPSLIDTLLPKAGRGAK"
     gene            13699..15186
                     /locus_tag="VLD60_14895"
     CDS             13699..15186
                     /locus_tag="VLD60_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB46300.1"
                     /translation="MKRRCSRAVISLFALIVVLPVTATPAEPPKTKDLPPIPLSVDEI
                     HAWIDRSHPLLKGAGTERVMARGKMLKALGAFEPTLINDTELERFISSSDPGKGTQTV
                     GFNDTLVEARHPWGFKYNAGVREVINGPANIPDLSFNNGNSQVLLGGSFPLLKGLLVN
                     PENAELQRSELADPRADIKIAQTRQDLFLAAATQFWDWVAAVKFAEVQRRAVGVAEDR
                     FRQVEGRAKAGAVAPLDVVEANQEVQRRREVAIAAQRLVEQEQLKLSMFLWENNAPVN
                     PPLDRAPDFPSQMLVPTPETIKAHKIQAKADRPEIKEIDIEAKLNNIDLELAKNNLLP
                     SLDAEAAPARAPEKFVLGLGYRFGLELRVPILQRKSRGEVLEAQGKADRFVLTQKFRE
                     QQVVIDVDNALSAIERAKERIAAAVESLRLAKTLEEGERFRFSLGATSVLFVNLRERN
                     SVDSEGQVIRAKSDYQKALALYQWAIGAWGKGTPSAIPVTYRARD"
     gene            15305..16966
                     /locus_tag="VLD60_14900"
     CDS             15305..16966
                     /locus_tag="VLD60_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB46301.1"
                     /translation="MQSSVDGRPNLFQSVLGYIGALLRLERRVLALIVSYSLAIGLFS
                     LIVPLTVQELTNTFAFAIQPVTIMTLAGVMIAALLFVGAFRTLQYYAVEVLERRLFAR
                     VAIGMAQQIPHLQFLGFKPRYANHFIETVFMQRALSVLLVDLINVVVGGAVGMTILVF
                     YHPYFLLYNALLLAGFNVVFLLSHGGLKATINMSHAKYDTLHWIQEISYNLLHFKATD
                     SQAILMQRTDELVTKYVETRQTRFGILVRQYLGSVGWQAVAHGGLIATAAWLLSIGQL
                     TLGQLVAAEVVVSGMLSSFDGVIKRMGHIFYFMTGLTELDFVLSLPKDQTSAMLSVPL
                     PDPTVHGIRVTCKDLTIHHPGVPPIFERFNLEVTPGEKIGIYASTTVAKTALARVLAG
                     LETPTAGVIRYNGVDLRHLDLSAINRCRGFMIDSQLTLFEGTIEDNIVLGRAYIPHSD
                     IRWALRFAELEEDVDALPFGLKTHVRAPGKILAPTHILRILLARAILSRPQILIFDGI
                     MHNMQPVMRETLIRRLCSKDEPWSVIFVSNDPNLTPHVDRRLILD"
     gene            16977..17759
                     /locus_tag="VLD60_14905"
     CDS             16977..17759
                     /locus_tag="VLD60_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HSB46302.1"
                     /translation="MPLVRWLRSFRPSGSQLLISLLVAGLGWLSGQALSRVDQDLRIM
                     YTEYTLGAADLAHISADVMRYRNTIIRALEADSQKEYERITESLPGQRARIQLAVDRY
                     AAAGLRVSRSGRSEPEDIEAVRRSLDQYFSVASTTIQLLTQEWTASSPSERERLRRKA
                     EEHAADNAGPKMIQVSLALDRLLDTVADVAKDMRDEGTRAIQSTSILLVGGSFFVALL
                     NLFLARPRRARRNDEEELAEAGHKPGRTPLPLPRASDQHYPA"
     gene            complement(17774..18493)
                     /locus_tag="VLD60_14910"
     CDS             complement(17774..18493)
                     /locus_tag="VLD60_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSB46303.1"
                     /translation="MTDTIRINKFFTQHGICSRREADRLIEAGRVTINQQIAKIGDQV
                     SQRDVVACDGQVIPWGAAPIYIKYHKPVGVTTTSEAHVPRNIIAEIGHTERIFPIGRL
                     DKDSSGLILLTNDGDIVNEILRTEHGHEREYEVTVDRPFDQAFLDQMAAGVVILGSPT
                     KPCRMERVTSKRFRIILTEGRNRQIRRMCQALGYRVVALHRIRIMHVTIAGLAAGAWK
                     PLTEDERTRLFRAIGRGTTVE"
     gene            complement(18509..19441)
                     /gene="speB"
                     /locus_tag="VLD60_14915"
     CDS             complement(18509..19441)
                     /gene="speB"
                     /locus_tag="VLD60_14915"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HSB46304.1"
                     /translation="MTLPAGWEDYQSNFLGLEEPWCHPDHAGVYVLPAPYEHTSSYIS
                     GSDRGPSAIIEASHQVEFYDEVLQCEPFREWGGIATVTPLDLKGQVDRQAVEAIEGFV
                     RPHVGGGRFVVTLTGEHTGALGAIRAHAKRYPNLCVVQIDAHGDLRQAYQDNPYSHAS
                     VMARVVDDGLPLVQVGIRSICPEEIERIHSTDRISTFFAAQLLDPFGPYEGKAARWIP
                     DVIKACRGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLNLITALANGPGILGMDI
                     SEIAPIEGFVAPQFCIARLIYRMLGRIKAGRRVH"
     gene            complement(19502..20071)
                     /locus_tag="VLD60_14920"
     CDS             complement(19502..20071)
                     /locus_tag="VLD60_14920"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HSB46305.1"
                     /translation="MVPTHMFLTRGVGVHREKLASFEQALRSAGVAYCNLVSVSSILP
                     PNCKIIPRKRGEKLLNPGEITHCVMARSETNERNRLVSASIGLAIPTDRRTYGYLSEH
                     HAHGETDEETGEYTEDLAAQMLATTLGVEFDPNIAWKEREQVFKMAGKIVRTLNITQS
                     AIGKPGKWTTVVALAVFIPLENLPKRGRR"
     gene            complement(20220..21659)
                     /locus_tag="VLD60_14925"
     CDS             complement(20220..21659)
                     /locus_tag="VLD60_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB46306.1"
                     /translation="MQGPRPFLIGGQWRQGDRVAPVHNPFTGKVLAEVAQATQADAEA
                     AVQATVDAAKTMGALPSHARYHLLQRIAGAIYDRRDEFARLITAEAGKPLTDAKREVS
                     RAVQTFTVAAEEAKRIPGDVIPLDWMPGTDSHLGILRRFPIGPVLGITPFNFPLNLVA
                     HKVAPALAAGNSILIKPAPQTPLTALLLGEVAVEAGLPPGGFNVLPCDNTVAEQLVVD
                     PRFKLLSFTGSAAVGWMLKAKCGKKKVVLELGGNAGVIVEPDADLEFAAQRCAAGGFG
                     YAGQTCISVQQVFVHHSVADLFTTKLLLQVARLKAGDPGDEATVVGPLIDQQAAHRIE
                     AWIGEAVSQGARVLLGGKRMGSVVEATVLSNVTPAMKVSCQEVFGPVVTVTPYRQFDD
                     AIQALNRSDYGLQAGVFTQDVNKVFHAFRQLEVGAVLANEIPTFRADHMPYGGVKDSG
                     IGREGVRAAIEDMTEPRLLVMNLKPPVVS"
     gene            complement(21687..21956)
                     /locus_tag="VLD60_14930"
     CDS             complement(21687..21956)
                     /locus_tag="VLD60_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46307.1"
                     /translation="MSDIQSFWPVSSREGEPAFWVCMSCLSEVFYRKVPMPDCPSCHG
                     VSTYEAFTIEAIADWGTEDLIAKANAAQQAANVEPAPASSVDSAL"
     gene            complement(22034..22681)
                     /locus_tag="VLD60_14935"
     CDS             complement(22034..22681)
                     /locus_tag="VLD60_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB46308.1"
                     /translation="MMRTGVLYRSAVVFLLGLALWQSNDVGAGSITDDGRVRGRADAP
                     ITLIEYSDFTCGYCLKFFKETLPQLQAKYIDTGKVKFVYRDYPRADRGVGVEAAVAAR
                     CAGAQGRYWAMHDRLFNEGGRLDSGAFKGFAKSIGLDPVTFGKCFDERRHLESIFQDR
                     QEANRWGFHGTPGFILMQTASGPTEKDPAVAIPGAFPFDDFAKEIDRMLTKVPGS"
     gene            complement(22678..23784)
                     /locus_tag="VLD60_14940"
     CDS             complement(22678..23784)
                     /locus_tag="VLD60_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HSB46309.1"
                     /translation="MGAREFRDGAKDGLEALEPLDPEKIGSFDDLLVAMRKTAFGGRR
                     LGEAYEVLSAMIDDPDCSVVMTLSGAMTIAKMGKIISTMIDHGMVQCVVSTGALIAHG
                     LSESVGKTHYRHHPSMTDEELFKKGYNRVYDTLEMESNLNYVEYVVAQTMKRINQDQS
                     LSSEILTRELGKTLAEEFDGAGILKSAFLKKVPVYIPAFTDSEMGLDVGTWAMGRVLD
                     QARAQTKGGSDIDILRAINKSHPSFNPYQDLNSYTNHIVSAKRLGIFTIGGGVPRNWS
                     QQVGPYIEISNMRLGLNVRPPRFQYGVRICPEPDYWGGLSGCTYQEGISWGKFVPPEE
                     GGRFAEVLSDATVVWPLLMVGLLERMNAKRAART"
     gene            complement(24218..24295)
                     /locus_tag="VLD60_14945"
     tRNA            complement(24218..24295)
                     /locus_tag="VLD60_14945"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24257..24259),aa:Glu,seq:ctc)
     gene            24507..25073
                     /locus_tag="VLD60_14950"
     CDS             24507..25073
                     /locus_tag="VLD60_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46310.1"
                     /translation="MDKNSVSMNNYFQTDSDMMDLDDAPELVVEEEKPEQGLELHIGS
                     NVFRTTNGVIKLQGKEQIVLEVQPDPATLLLTMDFYDEHGQRIGHLRRNTLSATGSSR
                     FAVSMNTTSDATLDDPFTVTISDRMTGGTVIEVYLFQRRKIRVTSGNFHTHKGDLVSI
                     SPHYCRLGTGLTMFGDVVESRGGTAVIG"
     gene            25199..25612
                     /locus_tag="VLD60_14955"
     CDS             25199..25612
                     /locus_tag="VLD60_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46311.1"
                     /translation="MSDIAKPSIQAFLVCDQVIEDSVTKKKSLIGLFTHLQAISFPFQ
                     HQQMGLYFCLTDAEGTYQFDIDLIYLNSEQLVCRANLPNIVIGDRLQISDFGINIPSL
                     IFPAPGRYEFRLRMEGHLIAQKDFNVMQMPTHQAA"
     gene            25744..27438
                     /locus_tag="VLD60_14960"
     CDS             25744..27438
                     /locus_tag="VLD60_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HSB46312.1"
                     /translation="MAEGTNASNFIRDIVAADRAAGKHGGRVVTRFPPEPNGYLHIGH
                     AKSICLNFGLAQETPGGICHLRFDDTNPTTEDPEYVQSIQDDVRWLGFDWQDKLFFAS
                     DYFEQLYEFAEGLIRKGAAYVDSLTAEDIRTYRGTLTEPGRNSPYRERFVEENLSLFR
                     EMRAGKFPDGTHVLRAKINMASPNINLRDPVLYRIRHAAHYRTGTAWCIYPAYDYAHP
                     LSDAIEGITHSICTLEFEDHRPLYDWVIEQAQTPHRPQQIEFARLNISYAVMSKRKLL
                     ELVEKNLVAGWDDPRLPTIKGLRRRGYTPEAIRAFCEHIGVAKRDAVVEMQLLEHFIR
                     EDLNKRSLRVMAVLKPLKVVLDNYPEGAVEELDAVNNPEDHSAGTRKVPFSRTLYIER
                     DDFLEDPPKQFFRLAPGREVRLRYAYIIKCVEVTKDPQTGEIIELHCTYDPSTKSGAA
                     EAQRKVKATIHWVSTSHALQAEVRLYNPLLIADLSKVPTDHDWTTYLNPQSLERITGC
                     QVEPSLRGAPPGSRFQFERLGYFCTDLDSTNDSPVFNRTVSLKDAWAKLDKRQPSR"
     gene            27640..28257
                     /locus_tag="VLD60_14965"
     CDS             27640..28257
                     /locus_tag="VLD60_14965"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB46313.1"
                     /translation="MLALLAITGPRAVDAGQAMTDESATEAVKRTITDLIYVLDDDTL
                     KQPEQSENRRREIEEIIRQRADYEEMAKRALGAPWSTLLKREQQEFVSLFVQLLRDTF
                     AGRITEHHDEQVAYLGEYREEDVYAEVHTRLKGRKIDTLIDYRMINRGGDWRVYDVVI
                     DGASIVSNYRSQFTSIIREVSYVGLVTRMKQKAIAVKVFERYSAP"
     gene            28388..28723
                     /locus_tag="VLD60_14970"
     CDS             28388..28723
                     /locus_tag="VLD60_14970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB46314.1"
                     /translation="MIECREHPRIPVDLQVYFSTTGNTSIREGTMFDLSAGGCAVASM
                     TTVQPGSAVRILIRATDLGSPITIESAAVRWSSHGEFGVEFVGLSEIDQSRLQRLLEI
                     RTSPHIRPS"
     gene            28765..28962
                     /locus_tag="VLD60_14975"
     CDS             28765..28962
                     /locus_tag="VLD60_14975"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HSB46315.1"
                     /translation="MNCPLCGQPTTWNGNPWRPFCSERCQLTDLGTWAAEEYRVPGPS
                     LTMELPSDENHETEEETGGKQ"
     gene            29198..30241
                     /locus_tag="VLD60_14980"
     CDS             29198..30241
                     /locus_tag="VLD60_14980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HSB46316.1"
                     /translation="MLPTKGFAAMTAKAALQPFSFARREIGPHDVLITISHCGICHSD
                     IHQARDEWGGSIFPMVPGHEIVGAIAGAGNAVKKFRVGDVAGVGCFVDSCRSCSACRE
                     GLEQYCEAGMLLTYSARDKNGQITQGGYSSQIVVDENYVLRIPKTLSPAGAAPLLCAG
                     ITTYSPLRHWGVGKYHKLAVVGLGGLGHMAVKIAKALGTEVTVFSTSESKRKDAIRLG
                     ATDFVVTTNPNAFAQPQRRFDFILDTVSAPHNYNSYVNLLKTDGTMILVGAPDKPTPL
                     EPFPLITHRRRIAGSVIGGVRETQDMLDFCAEHEIESDVEVIPIQQVNEAYERVLKGD
                     VRFRFVIDLMSLK"
     gene            complement(30274..30347)
                     /locus_tag="VLD60_14985"
     tRNA            complement(30274..30347)
                     /locus_tag="VLD60_14985"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30313..30315),aa:Gln,seq:ttg)
     gene            complement(30403..30476)
                     /locus_tag="VLD60_14990"
     tRNA            complement(30403..30476)
                     /locus_tag="VLD60_14990"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30442..30444),aa:Gln,seq:ctg)
     gene            complement(30546..31961)
                     /gene="gltX"
                     /locus_tag="VLD60_14995"
     CDS             complement(30546..31961)
                     /gene="gltX"
                     /locus_tag="VLD60_14995"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSB46317.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQQGVFVLRIED
                     TDQSRSTDESIQAIIEGMKWVGLDWDEGPFRQTERMDLYRSHAMHLLERGQAYWCVCK
                     AEELEARRKEAEAKGLSPRYDGRCRNLGITNPSDEAALRFKAPQEGQTVVDDLIKGKI
                     VFDNTVLDDLIIQRSNGYPTYNFSVVVDDALMNITHVVRGDDHLTNTPRQVPIFQALG
                     FPVPRFGHLPMILGSDKARLSKRHGATSIMAYKEMGYLSDAMVNYLVRLGWSHGDQEI
                     FTRQELIEKFSWDHVQKSAAVFNPEKLLWMNAEYIKTTPPSQIAGELVPLLENAGLKE
                     EVRAVSNQWLGQLVVLVKERTKTLTDMVMWVTPYFGQTVAFDEESVKKFLTPAIAPIL
                     GKLLARFEAFPTFSKQEWEESFKQLVEEEGMKMGQLAQPVRVALTGRAASPGLFEVME
                     VLGRDRTLFRLRKGIERASGV"
     gene            32085..32789
                     /locus_tag="VLD60_15000"
     CDS             32085..32789
                     /locus_tag="VLD60_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB46318.1"
                     /translation="MAEFIQLATVLSTTNLTPGVRQLILLPKERHISFQPGQWVSMKL
                     PVGRKPPLNRAYSMAAPATPSGELTLVFDHVVGGLGSEYLCRLKAGDEVAVSGPYGNF
                     TLPSPLDRELVFVARYTGLVPIRCMLKQLYAQRQTGSILLIAVAPGEDELLFHQELLT
                     LAVTHAGFRYLPLVAAGGEQQGVDLALSMLSPLIDGAPKVIPMLCGTKHFVRPLRAYF
                     TGAGYGRKEVKTETYD"
     gene            complement(32786..33202)
                     /locus_tag="VLD60_15005"
     CDS             complement(32786..33202)
                     /locus_tag="VLD60_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSB46319.1"
                     /translation="MGGTNPYIEKAAYELPKKSYTITYIAPDGNVTKVEVDPGRIPYG
                     QTGLPGSILDVAMGNGVDLEHVCGGVCACSTCHVIVKQGLESCSEGTDDEFDQLDEAP
                     MTTLQSRLGCQCVPNGTKDIVVEIPAVNKNLVREGH"
     gene            33342..33815
                     /locus_tag="VLD60_15010"
     CDS             33342..33815
                     /locus_tag="VLD60_15010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB46320.1"
                     /translation="MLKISKKADYALMALQHIASVQFGDVTPGRVVNTKEIAEEYNIP
                     LELLAKVLQALAKNGMIESHNGPKGGYLLARSARQITISQVLESIEGPLGITDCSHEK
                     DGEFCMQREHCNIRTPLLKVQDSIYQLLNNMTLQDMLGGTPLITIQSPTAQGVER"
     gene            33812..35029
                     /locus_tag="VLD60_15015"
     CDS             33812..35029
                     /locus_tag="VLD60_15015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HSB46321.1"
                     /translation="MKLPIFLDNHSTTPMDPRVLETMLPYFVEKFGNAASRNHAFGWA
                     AEEAVENARKQIAKLIKADPKEIVFTSGATESDNLALKGVLEMYKEKGNHIITSSTEH
                     RAVLDTAKSMEAKGLATVTFLPVDKQGMVNPDDVRNAITGKTVLISIMLANNEIGTIN
                     PVQEIGKIAKEKGILFHCDATQGVGKIPVDVQGMGIDLMSFSAHKIYGPKGVGALYVR
                     KKNPRVRIAPQMDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMAKESARLSAMRDR
                     LQAGIMAALDECYLNGHPTSRLPGNLNISFAYVEGESLLMGMKDIALSSGSACTSATL
                     EPSYVLRALGVGTELAHSSIRFGLGRFNTDDEIDYTIKKVIEIVTKLREMSPLYEMAK
                     EGIDLKSVQWAAH"
     gene            35057..36502
                     /locus_tag="VLD60_15020"
     CDS             35057..36502
                     /locus_tag="VLD60_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HSB46322.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKDEDGVGSLDAITWYGPDEKPAR
                     EELDTNKDGKPDVRRSYEAGALAREEQDADKDGRFELTTFFANGAPARSEADANGDGK
                     PDVLVTYAGGKRAEQQEDRDLNGAIDAVTRFGPDDKPAQVQEDSDGDGRLETTSYYES
                     GAVVRSERDRNGDGKPDLRVTYQGGKPASTEQDDDYDGSFEKRVAASSGGTQEQSEDR
                     NGDGKPDLRVVFDAKGQITRQEEDRNFDGKPDVISEFSGGVLRLVREDSKGHGCFDVE
                     QRLAADGKTVEEQAVDENGDCKLDLWSHFANGAPVWQAQDQRGAGFATVLTRFDGSGA
                     ATSQELISEGKRAPDTKIFLAPGGAVAAQCLDSDGNRTLDVRYRVEGGAVVEGLVDSN
                     KDGKADVRQVWSGGAIEHAEIDTNGDGRADVVQYFSGQNVVRQCEDEQYDGSVDRCFE
                     GQKVVPVSGVKDLSAKLPALDCGGFDPFWKR"
     gene            36583..36984
                     /locus_tag="VLD60_15025"
     CDS             36583..36984
                     /locus_tag="VLD60_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSB46323.1"
                     /translation="MRAALLTTLVVLLCLVVYAEYRRSPPPENAADAVAERTQSAPAG
                     APGGLLEPAPRTDPAAASIPSDASTRYETAAAAQPTPLRRHLVRPGDTLASLAARYYG
                     DPARGQEIYEANRDRIRDPERLHDGQELLIP"
BASE COUNT         8104 a        11085 c        10235 g         7600 t
ORIGIN      
        1 gagggcgaac tggtcaagct ccacttgccc cattatgcgc cagacgcagg taagccttag
       61 cccaaaaatc cccaaaattc gtcgactgcc ggtcaaaccc agctcaaatt ctagccaact
      121 gcaaaggaga ggccaattct aggaaagcaa ggaaaagcta tgagctaccg catctctact
      181 ggcaaaaata ccgggtgagc tcgtacgagg actgtcggag agaatcgggg gcatccatcg
      241 accaatccgc cggctggggg gttgatgcga cctgcgtctg gaagaccggt ccagggtgga
      301 taataacaaa aggaaccgaa ctgcccgttc ccgttctagg ggggtcctgt agacacaagg
      361 aacaggcgct gcctatttca cctttgagaa atcagcgttg atgcggactt ctttaccatc
      421 ctgaaggttg atcacgaaga cggacgccgc attgggatcg aaactttcat aagcaaacac
      481 cgctgtaaac cgggaacgat aggccgggcc gctgccttca ttcttacgtc cccgttctgc
      541 ctttccgata tcaatggcct tcactttctt ggggccctgc tgcaattcga tcagcgcgcc
      601 ttcggcaaaa tattcatcgt cgccgcagag ttggacttcc acttccatgt tcggcatatc
      661 gacgactttt tttatgaact cctccggcat gcggatttct gttttgcgtt tcttggactc
      721 agaggcctct tgccggccga aggcttccaa ccgatagcgt ttggttctga gcacggcact
      781 gggcccgcag gaatctttct caggatccgc acccacccgc gtgaccattg acgcgtgctg
      841 aaggactttc ttcacttctt caggagtatt ggccttgtcc atgggtgcgc gaccggcttc
      901 aagcgctttc tgggcttcct ctatcgacaa cttcacatcg atggcgaagg ccgggctgat
      961 tcctacgaca aggaacccga ccagacccgc caggctcaat atctccctgt tcctatcacg
     1021 gaccatgaca tcctccgtta caacaaaacg aacaaagaag gcggcattgt acgaaaagct
     1081 ctaccccttt gcaatcagcc gctgaagcgg acccagttct cgtagggctt ttctgtatag
     1141 aggacatgaa gatccacggg aatcctcaga cctgcctgtt cgagtaatgt ggcgacgacc
     1201 cggagcggaa gcccgacgac cgtggtgaaa tctccgttga ggcactctat caattctccg
     1261 ccacctccct gaatagaata ggcgccggcc ttcccgagac tatcccctgt agccagatag
     1321 cgttcgtgaa cctccgcatc gaaccgtttc atctggacgc gggcggtcga gagctgcgtc
     1381 atctcgatgc cacgcgccag gcaggtcact gtcaccgccg tgtgaaccag atgctcgcgc
     1441 ccggccatgc gctgaagcat cgctctggcc tctgccagat tcaccggctt acccaacacc
     1501 gctccctcca actcgatcaa ggtatcgctg ccgagaacaa tggcctccgg atcgtttttt
     1561 gcgaccgagc gcgccttgcc ttgcgaaaaa tatgcgacca gatcatccgc tgaccggccg
     1621 ggaacaacag attcttcaaa agccgggctc ctgacttcga aggggatctc gaggagggcg
     1681 agcaattctc gacgacgggg cgaagtcgac gcgaggacta cccgcatcgc gagaccatgg
     1741 aggcggtatc gtcagcgata tcggaagagg agatctcgga actggcttct ccatcagcaa
     1801 ttcggttggc ccggtaatcg gcaagcatcg cttccacatc ggcgacggag gaggcacgaa
     1861 acatactggc cctcagggcc gcggcatggg gaaaaccttt gcaataccac ccgaggtgtt
     1921 tgcgcatgcg ccggaattgg tcccgacccc actgcatctc gcataatctt gcatgttcga
     1981 gcaggacatc gaaacgagcc gccagcgagg gactccaggc ttccggtcca tcggcccctt
     2041 gtgtgcggac ggctttccga acgtcttcac tgccacggaa gaaccagggg gctccgagcg
     2101 ccgcgcgccc gactaacaca ccgtcgacgc cagtttcacg tacacgccgc aggacttctt
     2161 gcaaactctg cacatcgcca ttgccgaaga gcagcgttcc actgccacgg atcacctcag
     2221 ccgctcgggc aattgccgac cagtcggctt cgccccggta catttgttga agagttcgcc
     2281 cgtgaagcga aatcgccgtc ggtgattcgg ccagcaagtg ttcgatccag cgatccacga
     2341 tcacttcgtc atagcccagt cgcgtcttga ccgaaagtgg gatgctcttt ctgatcgttt
     2401 gagctgaact cccgcgtcgc tcgttcaaac gacgaacggc cgcagccctc accggcttga
     2461 taccggcagc ttccagcgat tgtcctcccg cccaatcctg gatcccttgg cgcgtcgcgc
     2521 gcatgatggc atgagcgaga tccggagtcc tgatcaaacc ggctcccgac ccagacgatg
     2581 ccacatttct ggaagggcag cccatgttga tatccaatcc gtcgaatccc agctcgcaaa
     2641 ctgcatgcgc ggcctggtag aagagctcag gatcctttcc atagagctgg gcgacgatgg
     2701 gacgttcttc ctcatgatag aggagcgaat ccaggagaaa ctccggaccg cggcagacgt
     2761 cgtggacgtg ggtgaattca gtgaacgtga cgtccggctt cccctgctgc gccacaatga
     2821 ggcggaatgt cgcgtccgtc actccatcca tcggcgagag tccgatgatc ggccttgcca
     2881 gtgattgcca gaaactcatg aggccggctc catcatcggt tgaactccat tgttggcctc
     2941 ggcataggcc aggcgcagcg tttccgcttc tcgttcggcc aggggcagag ccatcggcgc
     3001 tcgttctgcg acgaatgcca gcaccctctc gattttgagc aggaagaaat cgtacggttg
     3061 atcaaccggc cgggccgcct gaaaccacca ggtgacaaaa ccgtcaagtg cgacgccggc
     3121 ctgatccaac gcacggcagg tgtccggaaa catctccttc acgtcccctc cccagtggag
     3181 aatctcgtgc ggcacatagt cgtgtacata gcagtcaaga ccggcaggat cgtccacgac
     3241 atcttgccat tccggaccgc ttgggggctg ctgcccagat gcaatctgtg cgtaccggac
     3301 atagtcttct tgtaggcgtg accggtcgat gtcgttgaca gccccatgca taaccgtgaa
     3361 ctccgatcgt tgagggacgg gggccagggc tgccgatcgt ccggcaatgg cctgatgaat
     3421 catcgatccg aagttcttgc gggcctcgcg aagctcctcg acgatgtgtt cgatcggctt
     3481 cgtatctact tccagtgcca ccccccgcag gctgacaagg cccggatgag ccaacacttg
     3541 ctcgagtatc gtccacgata ccgcttgaat cggcgccgca tgggcatcga tccattctgg
     3601 atgagcgtcc tcccaccgac gacgggagag agactcgtga cggccaaggc ctgcaacgtg
     3661 aatctcgatg acccgctcca tgggaaattc atcgaggaat cgttcgatga actggtcaag
     3721 cgaaccttgc cgccgagccg ctgtgtaacg atacaccgtc cagagatgtc cgatgtccag
     3781 gacgagcccg caggatgtca gctccgttac aaaacggaag aattgtggga cggacattgt
     3841 tcccgccatg aaataggtga gcggcgccat ctccaagaga aacaatggtc cgaattcctg
     3901 tgccggtcgg catgcccggt ccatcttctc ctgaaccatg gcgatattgc tggccacaac
     3961 cctggcgctt tcggacgtat ataaaggtgg gaggtaggta ccgaaggaat accccgccat
     4021 ttgcttcgtg gcacattcat ggttgagcca cggactccgc aatattccaa gctgattggc
     4081 gacttcgttg atctcctcat cgataaatgc ctgcgttgag aaatccggct gagtgaccca
     4141 taggccttcg ccgtgatagg ccagtggaac tgtggagaaa tgcgtccgca ctctttggag
     4201 cgccgttgtg gtcgctcgaa aaatctcgag atagcccggt cgacacttct cctcttcaag
     4261 ccgtttcatc agctcaaaga gatcggggga atagacatca acggagagcc caagaccaca
     4321 cgtcggaatg accgccgcac gacacgtgaa ttcttgttca accggacgaa aacgagccac
     4381 cgactccccc ttgtacatcg acaatatttg gcctagtcta gtcttcgaag cccttgtttg
     4441 acaagggaac atagcaagct aggtgagggg accccgtctg ctataattgg cttacgccac
     4501 ggggggacaa cccgtcacga atcgttgcga atgaacgaat cgggagaggg ttctgcgatg
     4561 gccggtccta acgaaaaccg cccccaagcc cgcaatcgca acgatgtacg aactgtggta
     4621 gctcatatga tgacaccagg ggttgtgcaa gttcccggcg atgtctccgt cagcgaggca
     4681 gcgctgctcc ttgagcgaga gcatgcgccc tgcctgctcg tgaaggaaac cgatacaatc
     4741 ttcggtctta tgacgccaac cgatatcgtg aaaaaggtcg tcgcgcaggg ccttgagcct
     4801 catgaaattg aggtgcgtgc tatcatgacg cgccccgtac agtttattga atatgacgaa
     4861 gccctggaag aggcttcgtc gatgatgctg gcgactggtg cctcaatcct gatcgtgacg
     4921 aagaagaacc aacccgtcgg cgtcttgagc gcacaagacc tcatgctgac accgcccaga
     4981 cgacagacga aaatccccac tgtcgtcagc gtcgtggggg aggatctggc ccaccgacaa
     5041 gctcaaaatg ccacgatcgt ccaattgagc catgtcggcg cacgcatcga agcggccacg
     5101 agactccaca gtaaggcccg cgtggtcttg gcgttttccc tgccaggtca gcacactccc
     5161 ttaaccattc acgggaaagt catgacctgt gaatcccctg cgtccgacga cctcaagcaa
     5221 tcagggaagc gcgccgttgc gatcgagatt cagttcgccg acctctcgaa cgttgacctt
     5281 gcccgcctga aagcatgggc actgcaggcg atcccttctc aatctgatcg gggctaaatc
     5341 ctcaacatgt agttcttttg acctatcggc gatcagctga tacaattttt tctctagctg
     5401 aggcataccc gtcacctttg acgcgaatgg acaatcccat gaaagccacg ccgagccgtc
     5461 cccgtacgat gccgtcgaat gacgagatgc tgaaccaaat tcggcaactg ctcgagcgtc
     5521 ctgaagacga tatccaggcc gcgctgatca aagaactctt ggccagcatg cttcgtctcc
     5581 atgacgctca cctcgattcg ctcgacctga agatcgtcaa ccgagcagtc aaagagctgc
     5641 gacatgcctt tcgcgtattt catgactacc gtgatcgtaa gaaaatcagc atctttggat
     5701 cggcgcgtac cgcggcagat gacccgaact atcagatcgc ctttgatttc gcgcggcgca
     5761 tcgttcagga aggattcatg gtcattacag ggggcgccga cgggatcatg cgcgcatccc
     5821 aggagggcgc cggtcgcgag aacagcttcg gcgtgaacat catgctgccc ttcgagcaag
     5881 gccctaatac cactattgcg gacgacccga agctggtgac cttcaagtat tttttcaccc
     5941 ggaaactcat ctttcagaaa gaagccaatg cgattgcact attccccgga ggcttcggca
     6001 cccacgacga aggatttgaa attctcaccc tcgctcaaac cggcaagagc gatcctcagc
     6061 cgattgtctg cctccaagct ccgggctgcg actactggga tgagtggtac aggtttgtca
     6121 cgggccaact cctagatcga aaactcatca atcccgaaga cctcaatctc ttcaagatca
     6181 ccaattctgt tgacgatgcg atcaatgaga tcagaacgtt ctacagacga ttccactcga
     6241 tccgatacgt cggccgtcag ctggcaatgc gattgacgat gcgccccaca tccgatcaag
     6301 tggcggcgat tcacgagcag ttttcggatc tcttattgga aggcacgttt gaacttcgag
     6361 atgcactacc ggaggaattg gacgagccgg acttgaaaga cctgcctcgc cttgtttttg
     6421 cctccaaccg tcgaagcgca gctcggcttc gtcaactgat cgaccaccta aatcacctct
     6481 agcgcccttc gcaatcaggc ttttgtatcg gaggagcccg tggtatgctc cgccagcatg
     6541 gccacttccc atccacaacg acgtttctct cagcccccca cagtggtgct ctaccatgcc
     6601 gaatgcgcgg acgggttcgg cgccgcttgg gccctgtggc gtcaatttcc tgaggcacgg
     6661 tttcttcccg taaaacatgg caatccaccg ccttccggct tgaaggacca gcgtgtggtg
     6721 atcgtggatt ttagctacgc ccgcgagacg ctggaaacca tggcggagca ggcggacgac
     6781 ctcctcatac ttgaccatca cattacggcc gaaaaggcgc ttaccggatt gccctatgcc
     6841 tatttcgatt tgaaaaagtc cggggctgtc ctggcctggg aatgggcgca cgatcgcccc
     6901 gctccctggc tcctcgatta tattcaagac aaggatctct ggacttgggc gctgccgcag
     6961 agccgcgaga tcaatgccgc gatcgcctca catccgtttg agtttcagac gtggaacagc
     7021 tttaggcaaa aggatttgga gcaggagggt cgtgccatct tacggtacga gcatgagctc
     7081 gtgagcaaac tcgccggaca ggctgtgccg gtcgaatttc aaggcgtcac cgtcccctcg
     7141 gtgcaaagcg ccattctgac cagccagatc ggcgaacggc tatctgctga tgcgcccttt
     7201 tgcattatct ggcacgatcg ggacggacgg cgctactaca gcatgcgatc acgggaagac
     7261 ggaacggacg tcggcgcgat cgctgcctcg ttcggcggcg ggggccacac gcatgcggct
     7321 gggttttccg taccactcgg ccttgacggg tcgctgcccg acaatcctgc tctcccccgg
     7381 tcggtgatcg atcgccggcg cacctcagcc taagccggcc attgcatgat tcccctccac
     7441 gacgacaacc cgacggaacg gactccggtt gtcacgatcg cattcatcgt cgcctgcgtg
     7501 ctggtgttcc tgtatcaggc cagtctccct tcccaacccg gtgagatcct cgtgtttcag
     7561 tatggcgcaa ttcccgcgat cgtgtttgga cgtgcggatt tgccggaagg tattgtcgcg
     7621 atccctgcct acgctacctt gatcaccagc atgtttctgc acggtggctg gatgcacttg
     7681 atcggcaaca tgctgtacct gtgggtgttc gggaacaaca tcgaagacgt tatgggccat
     7741 gcgagatatg tggtgttcta tctcacctgc ggaatcctcg ccgccttaag ccacgccgtg
     7801 accgaaccct cttccaccgt tccaatggtc ggcgcaagcg gcgctatttc gggcgtgctc
     7861 ggagcctatg tgctgctgtt tccgcgcgcg cacgtgcttg tcctcatgcc aggaatcggc
     7921 atgacgaggg tcgcagccgg aatcgtgctt ggcatgtggt tcgtgatgca actcttgagt
     7981 ggcggtatga gcatcgggag taaaggcgga ggagtggcat tcttcgccca tatcgggggg
     8041 ttcatcaccg gcatggtgtt gatcggatta ttcaaacgtc aggatgtgcc gttctttgcg
     8101 ccgcctcgga cccgccattg gaattcgtga gaagtctcct gcagaatctt gctacgcgtc
     8161 gatcagctcc cgaacctttt cgactaatcc tgtctgtcta tacggccgcc tcaagagata
     8221 tcgccggttg atgcggtggg agatgatcgc ttcatcgtcg tagccggaca caaataaggc
     8281 cttcattgtc gggtactgct gcaacaaccg tttggccaac tcgcggccgc tgatctccgg
     8341 catgatgagc ccgctgaccg tgacatgcac ggctccggta tagtgttgcg ccagcatgag
     8401 ggcttcgacc ggcgagctgg cctccagtac ccggtatcga tggcgctgca acgtcgagag
     8461 tgccagcttg cgctcaatct cctgaggttc gaccaacaag accgtttctt cacccttggc
     8521 cactagggct gggacgatcg actgactatt ggaaggacca gatgcaactg cagggagaaa
     8581 aatgcggacc gttgttccat gaccaggctg acttctgacc tccacctgcc ccccgtgttg
     8641 tttcacaatg ccatagacgg cggtcagtcc tagccctaca ttggtctctt tggtggaaaa
     8701 gaatggctcg aacatccgtg actgcgtgtc cctcgtcatt ccgcttccgg aatctcgcac
     8761 ctgaatccgc acagtcgaaa gatgcctgct cgtccccgaa agcggcgtgc cgtccactcc
     8821 accctgcgcc tgtgcgacct cgatcgtcag ctgtccgccc cgaggcatcg cgtcacgggc
     8881 attgacggcc aggtttaaca gcacctgctc aagtccttca cgatccacct ccacttcgag
     8941 cgggccttct gcaattgaga tccggcagtt gatcccatgg ggcaacagac cgcgaatgac
     9001 atcgctcagg tcttcgatca ccgtccgcat cgagagcaac cgtcgaaact ggcggtgctg
     9061 aatatcgagc atggccagtt gtgccgtcag ggcagcagcc tgttcgccgc tccggaaaat
     9121 ctgatcgacg ggttcataca aaggatcgtc ctgtttcaac cgggacaaca ccacgccggt
     9181 ctgcttgccg atgacggaaa acagatggct gaactcgctc gcaatgccgt tcgtgatcct
     9241 gccgatcgct tccatctttt gcgcttcaaa caactgccgt tccagacctt cccgttccga
     9301 cctttcctgc tgaaccgagg cattggcctt cgacagatcg tctttgagtc ctttgatgcg
     9361 aatctccaac tgctcgcgag aagcttgaag ggttctctcg gcgctcctca actcgtcaat
     9421 actctcaagt aatcgcgctt tctgcctccg ctctaccccg attgtttcca gcgccatccc
     9481 gtagaagatc gccatcacca gaaggacggg cacacccagc agttgcccga ctgaaataga
     9541 gccgctttgc accacgcctt ggtacagcac gacgccatag cctgcgcaga ggaagagcga
     9601 gaacgcgatg acatgggata gccgacgcag agacgccgca atcagcatga ggatgaaata
     9661 ggatatgtac aggtcggatc gtgcattgcc tgagagatag atcgtccctg tcaccaaggc
     9721 cgtatctacc gcaatgagcg tgccgctgaa ccaggcagcc tgcaggacgg aaaccggcac
     9781 agccaacaaa cagccgacca acagaaccaa tcccccgacg atgaattcac tcgttgtgcg
     9841 tgtcgcaatc ggctcggcgc cgaacagcaa ctgatacgcc agcatcacac cgacgagcga
     9901 ctggaggatc acataccttg cccgggacac gggcgtctcg gggccttcgg gcttcgcagg
     9961 ccgctcggca agatactcgt ctgaataggt caaattgttc cgcatcattc tccctctgga
    10021 cttccgaggc aggggaatgg agcaagtcct gtaccggtgg accaactgaa cagtagcctg
    10081 tttttcctca ttccagggct ccggcccttg aacagtttcg gccaggacct atagaaactc
    10141 acaaaatgga acgattgggt cgatggacga aacgcgccac tcaacaagat ttttcaggaa
    10201 ctcattcggc gggattagcg cggccgttca ctgatcaggt tttgacgcaa gcgagtggcg
    10261 agaccttggg cggtgtcacg ttgatcagcg agaaatgtca catgccccat tttccggcga
    10321 gggcggatcg tgcgtttacc atacagatgc acgtgagcgg ccggaaggtt cagaagggca
    10381 cgacattcgg ctcctgttcc gattgtcgtg gcgtcatctc cgatgaggtt aaccatggcg
    10441 gcagggctca acagccggac ctctcccagc ggcatcccgc atatcgctcg aacctgttgt
    10501 tcgaactgag acacactgca cgcatctaat gtataatggc ccgaattatg agggcgggga
    10561 gcgacttcat tgataagaag cgttcccccc ggcatttgaa aaagctcaag gcagaacagg
    10621 ccgacccctt gcatccgttc catcaccgtc cgcgccaagc gctccgcttg ctccgccaca
    10681 gcctggggga cggcggctgg cacgagcgtc gcgcgaagaa tcccttcttc atgctcgttc
    10741 tcgacgggag gatacacgac agactgtccg tcggctccac gggccaccaa aatcgagagc
    10801 tcccgttcga acggcacgaa ggcttcaacg atccaccgca tcccgggctg agccgccgca
    10861 cccaacaacc gttcaacctc gggtacgtcc gattcgcttg cgattctcca ttgccctttc
    10921 ccatcgtaac cggtcgtcgc cgtcttgcag agggcagggc taccgatttg tcgaatcgct
    10981 tcagccaatc cgtcggggtg cgtcatggca gcaaaacgag gaacgggcaa accccggtct
    11041 gcgagaaacg tcttctggat cagacgatcc tgaatgatac tcaagacggc gctggagggt
    11101 cgtaccggct tcatctcttc gagttcccgg cacagttgcg atgggacatt ctcccattcg
    11161 taggtgacga cgctcaccat gtctgcaaac tgagcgaacg tctgccggtc ggtaaatggt
    11221 tttggaaatg aacgggtggc cacacggtgg gccggagcat ccggatcggg atcccagacc
    11281 gcgacgcgaa atcccaagcg tgcagcggcg ctggcaaaca tcgcacccaa ttggccggcg
    11341 ccgaggacgc cgagcacgga tcctggctca ataggcgaag gagtcacgat cggcgacgtg
    11401 gaggggaaaa cgtagagccc cctgcgaccg aacctttcgc ttccggcgac cggagcacgg
    11461 tgtctgtctg ggttttacgg aatgacttga ctcgggccgc gatggccgga tagcggccgg
    11521 ccaaaatctg cgcagcgagc aagcccgcat tgtccgctcc gccaatggcg acggtagcca
    11581 caggaatgcc tcggggcatc tggacgatcg agaggaggga atcaagccca cgaagatttt
    11641 ccgttggaat cgggacaccg ataaccggca ggtgggtctt cgctgccaac atgcccggta
    11701 ggtgagcggc ccctcccgcc ccagcaatga tcacttcaat cccccgggca gatgccagct
    11761 ccgcataggc aaacaggcga tccggggtgc gatgggctga gacgaccaag agttcgttgg
    11821 gcacgccaag ctccgtcaat acagctaccg ctttctccaa gatcggaaaa tcggatttgc
    11881 tcccaccgag cactcccacc agcggctgcg cggaggcggt ccggcgattt gccgatcgcc
    11941 tgctcgtcgg tcttctggat cgtgccatga acgtgaaact cccttaattt acgacctgct
    12001 cgagcgccca cattaacgac tctgacctac ctggtcaaga tcgcatggat cccaacggtt
    12061 ctccttcctt aagagaaatt gacccgccgg gccactttcc tctatgatta acgtgatcca
    12121 gatcggtccc attccgcacc cgatgagggc tcggtggtag gtctagggca gagtttccgt
    12181 cagaaagtcc ggtcgcttgt ccaacaccgc agtggcttgg atgagatcgc gatcgacgac
    12241 atagccacct caagcaagct tcactcttgg gaagcagtcc aaatccctga acggctgaag
    12301 ttttcctccc gtctcgccat caagcttgtt ctgctgtttc ttctgatcat cgcgttcgtc
    12361 ccctggacgc aaaccattac cgtcaccggg caattgtccg cctatacgcc ctacgaacgg
    12421 ccccaagaca ttgaagccca gattaccggt cgcatcaaga aatggcatat tttcgaaggt
    12481 gtccgtgtga aacaggggga tctgatcctc gagctcgatg actatgaccc gagctttatg
    12541 tctccagacc tcctctctct gctcgatcaa cggaagaaag ccctctcaga cactcgcaag
    12601 gctgcgctcg ggcgcgcgga tcagttggat aagcgcatta aggaaatgca gaatctcgtt
    12661 aaggctgcgg tgccgtcggc tcaggctcgt gtcgtcgaag ctgagaacaa ggttcgcgaa
    12721 gcctaccaga aggtggaggc ggcaaaaatc gccgtcgcca ccgccgaatt gaatgtcgac
    12781 cgtcataagc agctcgcgga acagggcctt gtgtcgcaac gggaattgga agtgaccatc
    12841 cagtccgctc tcgccacgaa ggcggacttg agcggtgcgc aggctaatct caaggcggcc
    12901 gaacagagca tgaaggcact cagcttcgga cgtgagcaag tcagtgccga agtcctgcag
    12961 cgcttgctcg atgccgaagc cgcccgcgat gcttcggtcg ccgaagccgc caaagcaacc
    13021 gatcagctgg ccgatatctc gttgcggatg tccaacgccg agcaacgccg gatcgccagc
    13081 cgcatccttg ctcccatcga cggtaccgtg gtgaaaatgg ccgaaaccgg tgccggtgaa
    13141 acggttcgac cgggcgaaaa gctcgtcagg atctctccgg caagcgccga taaggccgtt
    13201 gaaatgacgg ctgatggaat cgatgccccc ctgctcaatg tcggacgcaa agtgaagatc
    13261 ctgttttacg gcatcccggc cattccactt ccggcctggc ctgaattgat ggcaggcacc
    13321 tatgtcggcg tcatcaaggt catcgaccaa gtcgacgatg gaaaggggaa cttccggttc
    13381 tgggtcgtgc ctgatccgga tgaaaaacca tggccccctc aagagcacgt gcgccagggg
    13441 accaaggtga tggggtgggt catcctcaac cgtgtcccgt tgtggtacga actctggcgc
    13501 cgtttcaatc tgttcccacc ggactatcaa gaacgtccgc cgagcctgat cgacactctc
    13561 ttgccgaagg caggacgagg tgcgaagtag gccagtcgca gcccatcgca gccctctctc
    13621 atctcaacta ttgctattgc cgcagggggt agatcctcgc ggcgggatgt tatccgcgta
    13681 atgaaaggag cgtatcccat gaaacgtcga tgctctcgtg ctgtcatcag cctgttcgca
    13741 ttaatcgtcg tcctgccggt gaccgcaact cccgcggagc cgcccaagac gaaagacctt
    13801 ccacccattc ctctcagcgt cgatgagatc catgcctgga tcgatcggtc gcaccctctc
    13861 ctcaaaggag ctggcacgga gagggtgatg gcacggggaa aaatgttgaa agcgctggga
    13921 gcatttgagc cgacgcttat caatgatacg gagctcgagc gattcatctc cagctccgat
    13981 cctggtaaag ggacccaaac cgtcggcttc aacgatacat tggtggaagc gcggcacccc
    14041 tggggattca aatacaacgc tggcgtccgt gaagtgatca acggacctgc aaatattcct
    14101 gacttgtcat tcaacaatgg aaacagtcaa gtgctccttg gggggtcctt cccgcttttg
    14161 aaaggcctcc tggtgaaccc ggagaacgcc gagttgcagc ggtctgaatt ggcagaccct
    14221 cgggcggata tcaaaattgc ccagaccaga caggacctct tccttgccgc cgccacgcag
    14281 ttctgggact gggtcgccgc cgtcaaattc gccgaggtac agcggcgagc cgtcggagtg
    14341 gccgaggatc gtttccggca ggtggaaggt cgcgccaagg ccggagccgt cgcccctctc
    14401 gatgtggtcg aagccaatca ggaagttcaa cgccgtcgcg aggtcgccat cgccgcgcag
    14461 cgcctggtgg agcaagaaca actcaaactc tccatgttcc tctgggaaaa caacgctcct
    14521 gtgaatccgc cgttggatcg ggcaccggat ttcccctccc agatgttggt gccgactccc
    14581 gaaacgatca aggcccataa gatccaagcc aaggcagatc ggccggaaat caaggaaatc
    14641 gacattgagg ccaaacttaa caacatcgat ctggaattgg cgaaaaacaa tcttctcccc
    14701 agcttggatg ccgaggcggc cccagcacgt gcaccggaaa agttcgtcct gggtctcggc
    14761 tatcgcttcg ggctcgagct cagagtgccg attctccaac ggaaaagccg gggagaagtc
    14821 ctggaagccc agggcaaggc tgatcgattt gtcctgaccc aaaagttccg tgaacaacag
    14881 gttgtcatcg atgttgacaa cgccctgtcg gcaattgagc gggccaaaga gcggattgcc
    14941 gcggcggtgg aatccctgcg cctggccaaa actcttgagg aaggcgaacg gttccggttc
    15001 agcctcggcg cgacgagcgt tttgttcgtc aacttacggg aacggaacag cgttgattcg
    15061 gaaggtcagg tcattcgcgc taaatccgat taccagaagg cgttggcgct ttatcaatgg
    15121 gccattggag cctgggggaa gggcacgcct tcagcaatac cggttactta tcgggcgcgt
    15181 gactgagacc ggaagaagtg gctttttctc ctgtactttg atagcgttac aattggtgtt
    15241 gtttgcaggc ggctagcctc tgacaagttc aacgacgtca gattgaagaa gaaggcgggt
    15301 gtccgtgcag agtagtgtcg acggtcgacc aaacctcttc caatcagtgc tcgggtacat
    15361 cggcgcactg ctccgccttg agcgccgggt ccttgccctg atcgtttcct actccctcgc
    15421 tatcggtctg ttctccctga tcgttccatt gacggttcag gagctgacca ataccttcgc
    15481 ctttgcgatc caaccggtca cgattatgac attggccggc gtcatgatcg ccgccctgct
    15541 gttcgtggga gcgttccgca cattgcaata ttatgcggtt gaagtattgg agcggcgact
    15601 cttcgcccgc gtcgcgatcg ggatggctca gcaaatcccg catttgcagt ttctcggctt
    15661 caagccccgg tacgccaacc attttatcga aacggtgttc atgcaacggg cgctgtcggt
    15721 tctcctcgtc gacctcatta atgtcgtcgt gggaggtgcc gtcggcatga ccatcttggt
    15781 cttctaccat ccctacttcc tgctctataa cgcgctcctt ctcgccgggt tcaacgtggt
    15841 cttcctgctc tctcatggag gcttgaaggc aaccatcaac atgtcccatg ccaagtacga
    15901 caccctccac tggatccagg agatctcgta caacctgttg catttcaaag ccaccgacag
    15961 tcaggcgatt ctgatgcagc ggaccgatga gttggtcacc aagtacgtcg aaacccggca
    16021 gaccagattt ggaattctcg ttcggcaata cctgggttcc gtcgggtggc aagccgtggc
    16081 gcacggaggg ttgattgcga cagccgcctg gctgctctcg atcggccagc ttaccctggg
    16141 acaactcgtc gcggcggaag tcgtcgtcag tggaatgttg tcgagtttcg acggcgtcat
    16201 caaacggatg gggcacatct tctacttcat gacgggactc accgagctcg atttcgtcct
    16261 ctcgctgccg aaagatcaaa cgtcggcgat gctgtccgtc ccgcttccgg atccgaccgt
    16321 acacggcatt cgcgtgacct gcaaggatct cacgattcat catccgggcg tgccgccgat
    16381 ttttgagcgt ttcaatctgg aagtgacccc tggcgagaag atcggcatct atgccagcac
    16441 caccgtcgcg aaaacagcgc tggcgcgcgt gctggcaggc ttggaaacac ccaccgccgg
    16501 ggttattcgg tacaacggcg tcgatcttcg tcacctcgac ttgagtgcca tcaaccgctg
    16561 tcgcggtttc atgatcgatt cccaattgac gctcttcgag ggtacgatcg aagacaacat
    16621 cgtgctgggc cgtgcctaca ttccccacag cgacattcgc tgggccttgc gattcgccga
    16681 actggaagaa gacgtggatg cgctcccttt cggattaaag acccacgtcc gggcacccgg
    16741 caagatcctg gccccgacgc acatcctacg cattctcctg gcgcgtgcga ttctgagccg
    16801 gccgcaaatt ctcatcttcg acggtatcat gcacaacatg cagccggtga tgcgcgaaac
    16861 cttgatccgc cggctctgtt cgaaagatga gccctggtct gtgattttcg tctcgaatga
    16921 cccgaacctg acgccgcatg tcgatcggcg cctaatcttg gattgatgag gactacttgc
    16981 ctctcgtacg gtggttgcga tcgttccgtc ccagcggatc acagctttta atcagcctgc
    17041 tggtcgcggg gctcggctgg ctcagcggac aggccttgag ccgcgtcgat caagatctcc
    17101 gcatcatgta caccgaatat acgctcggcg ccgcggatct ggcccacatt tcggcggacg
    17161 tcatgcgcta tcgcaacaca atcattcgcg cgttggaagc cgacagccag aaggagtacg
    17221 agcgcattac cgaatcgctg cccgggcagc gggccaggat tcaattggcc gtcgaccgct
    17281 atgccgcggc agggctgcgc gtctcccgaa gcggccgtag tgaaccggaa gatatcgagg
    17341 ccgtacggcg gagcctcgat caatatttct ctgtggccag taccacgatc caattgttga
    17401 cgcaggagtg gaccgcctca tccccgtcag aacgagaacg cctccgccgc aaggcggagg
    17461 aacatgccgc agacaatgcg ggtccgaaaa tgattcaggt aagtctggcg ttggaccgcc
    17521 tattggacac cgtagcggac gtggctaaag acatgcggga tgaaggcaca agggcaattc
    17581 aatccacgag catcctcctt gtcgggggaa gcttctttgt tgccctgctc aatttgtttt
    17641 tggccaggcc ccgtagggct cgccgaaatg atgaagaaga gctcgctgaa gctggccaca
    17701 aacccggccg aactcccctt ccactcccac gagccagtga ccagcattac cctgcgtaag
    17761 gatctcgcct cgctcattcc accgtggtac cccgtccaat ggcacggaag agtctggtcc
    17821 gctcatcctc tgtgagcggc ttccacgccc ctgctgcgag cccggcaatc gtcacatgca
    17881 tgatgcggat acgatgcagc gccaccacgc gatatccgag ggcttgacac atgcgccgga
    17941 tctgccgatt tcgtccttcc gtcagaataa tccggaaccg tttggacgtc acccgttcca
    18001 tccgacaggg tttggttggg ctcccgagta tgaccactcc tgcggccatt tgatcgagaa
    18061 acgcctgatc gaatggacgg tcgaccgtca cctcgtattc ccgttcgtgg ccgtgctcgg
    18121 tccgtaaaat ctcgttcacg atgtctccgt cattggtcag aaggattaat cccgacgagt
    18181 ctttatcgag ccggccgatc gggaaaatcc gttcggtgtg gccgatctcc gcgatgatgt
    18241 tgcgcgggac atgcgcttcg ctcgtcgtcg tgactcctac cggtttgtga tacttgatat
    18301 agatcggcgc ggcaccccag ggaatcacct gcccgtcaca ggcgacgacg tcccgctggc
    18361 tcacttgatc cccgattttg gcgatttgct gattgatggt cacacgcccg gcctcgatga
    18421 gccgatcagc ttcgcgccgc gagcagatac cgtgttgcgt gaagaatttg ttgatgcgga
    18481 tggtgtcagt cacaagaggg gtatcggatt agtggacccg gcgaccggct ttgatccgcc
    18541 ccaacatgcg gtagatcagc cgggcgatgc aaaactgcgg cgcaacgaac ccttcgatcg
    18601 gggcaatttc gctgatgtcc atcccgagaa tgcccggccc gttggctaac gcggtaatga
    18661 gattcaaggt atcataccac cccagtccgc caggctcagg cgtccccagc gccgggacca
    18721 acgaagcatc gaggccgtcg caatcgaacg tcaggtacac cggcccgcgg caggccttaa
    18781 tgacgtcggg gatccaccgc gctgctttcc cctcgtacgg accgaaagga tccaagagct
    18841 gtgcggcaaa aaacgtcgag atgcggtcgg tgctgtggat tcgttcgatt tcctccggac
    18901 agatggaccg gataccgacc tgtaccaacg gcaatccatc gtcgacgacg cgcgccatga
    18961 cactggcatg gctgtacgga ttgtcctggt aggcctggcg caaatcaccg tgcgcatcga
    19021 tctggaccac gcacagattg gggtaccgct tcgcatgggc tctgatcgcc cccaatgctc
    19081 cggtgtgctc gccggtgagg gtgacaacaa accggccgcc cccgacgtga gggcgcacaa
    19141 acccctcaat ggcttccacg gcttgacgat cgacttgccc tttgagatcg agcggagtga
    19201 ccgtcgcgat tccgccccac tcgcgaaagg gttcgcattg cagcacttca tcgtagaatt
    19261 ccacttgatg cgacgcctcg ataatcgccg atggccctcg atcggatcca gaaatgtagc
    19321 tggaggtgtg ctcgtacggt gcgggaagca catagacacc cgcgtggtcc gggtgacacc
    19381 acggttcttc aagaccaagg aagttgctct gatagtcttc ccatccggcg ggaagcgtca
    19441 tggatgtgca ctcacaggcc gtgggataag acgcccgggc agccttgtcg gggaggttga
    19501 attagcggcg gcctcgtttc ggcagattct ccaaggggat gaacaccgcc aaggcaacga
    19561 cggtggtcca cttcccgggc tttccgatcg ccgactgcgt aatgttcaat gtgcgcacaa
    19621 tcttgccggc catcttgaac acctgctctc gttctttcca agcaatgttc ggatcgaact
    19681 cgacccccaa ggttgtcgcc agcatctgcg cggcaaggtc ttcggtgtac tcgccggtct
    19741 cctcgtccgt ttcgccatgc gcatggtgtt cggaaagata tccgtacgtg cgacggtccg
    19801 tcggaatcgc caacccgatg gaggcggaca ccaaccgatt tcgctcgttg gtctccgaac
    19861 gggccatgac gcagtgcgta atttcaccgg ggttcaagag tttttcgcct cgcttacgag
    19921 ggataatttt gcaattcggg gggagaatcg aggagacgct gacgaggttg caataggcca
    19981 caccggcgct tcgcaacgct tgctcgaagg aggcgagctt ttcccggtgc acgccgactc
    20041 ctctggtcag aaacatatgc gtaggtacca tcgtctcttc tcccttgctt cgtccggtca
    20101 actgcgcagc ggctgctctc tcgcagaatc atcaggccca ccacgaaaat tctatgggca
    20161 cagggccgct gttgtatcaa gaatgaccgc ggttcgcaat atcccgcgac gtttttttgc
    20221 taggaaacga ccggcggctt tagattcatc acgagcagcc gtggctccgt catatcttcg
    20281 atagccgcac gcaccccttc gcggccgatt cctgaatctt tcaccccgcc atagggcata
    20341 tgatcggctc ggaacgtggg gatctcgttg gccagaaccg ctccgacctc caactgccga
    20401 aaggcgtgaa acactttgtt cacatcctga gtaaacacac cggcctgcaa tccgtaatcc
    20461 gagcggttca acgcctggat cgcgtcatcg aactgacgat agggtgtcac cgtaactacc
    20521 ggaccaaaga cctcctgaca ggacaccttc attgccggag tgacgttcga gagcacagtc
    20581 gcctcaacca ccgaccccat gcgtttaccg cccaagagga cacgggctcc ttgcgagacc
    20641 gcttcaccaa tccaggcttc gatgcgatgg gccgcctgct gatcgatcaa cggcccgacg
    20701 accgtggcct cgtcgcctgg atcgccggcc ttcagccgag caacctgcaa tagcagcttc
    20761 gttgtaaaga gatcggcgac tgaatggtgg acgaatacct gttggacgga gatacaggtc
    20821 tggccggcat agccgaaccc gccggctgcg caacgttgtg cggcgaactc caaatcagcg
    20881 tccggttcga caatcacgcc cgcgttccct ccaagctcca atacgacctt tttcttcccg
    20941 cacttggctt tcaacatcca tccgaccgcc gcactgccag tgaagctgag cagtttgaag
    21001 cgaggatcga cgacaagttg ttcggccacc gtattgtcgc agggcaacac attgaaaccg
    21061 cctggaggaa ggccagcctc caccgccacc tcccccaaca gcagcgcggt cagaggagtt
    21121 tgcggagccg gcttaatgag aatcgaattc cctgcagcca acgccggagc gactttatgg
    21181 gcaacaagat tcaggggaaa attaaacggg gtgatgccga ggacgggtcc gatcggaaac
    21241 cggcgaagaa tgccgagatg ggaatcagtc cccggcatcc agtcgagcgg aataacatcg
    21301 ccgggaattc gcttggcctc ttccgccgcc acagtgaacg tctgaaccgc ccgactgact
    21361 tcccgtttgg catcggtgag cggcttcccc gcctcagctg tgattaacct ggcaaactcg
    21421 tcacgtcgat cgtagattgc cccggctatc cgttgcagta gatgatagcg agcgtgagac
    21481 ggaagggccc ccatcgtctt ggccgcatcg accgttgcct ggacagccgc ctcggcgtca
    21541 gcttgagtgg cttgcgcgac ctcggccaga accttcccgg tgaaggggtt gtgcacagga
    21601 gcgaccctgt cgccctgtcg ccactgaccc ccgatgagaa atggacgtgg tccctgcaag
    21661 gagacgctcc actggtggaa gaggtcctac aatgcggaat cgactgagga tgcgggagcc
    21721 ggctcgacgt ttgccgcctg ctgcgcggca ttggccttgg cgatcaagtc ctccgtgccc
    21781 cagtcggcga tagcttcgat cgtgaaggct tcgtacgtcg acactccgtg acaactcggg
    21841 caatcgggca tcggcacttt gcgatagaag acttccgaga gacaagacat gcacacccag
    21901 aaggctggct caccttctcg ggaggaaacg ggccagaatg actgtatatc agacatacgg
    21961 tgatccctcg tgattgagat aacgtgaatg ctggttatgg cttaccagaa cttgctacat
    22021 gaaaccgttc tcgttatgaa cccggcacct ttgtgagcat acgatcgatt tctttagcaa
    22081 aatcatcgaa cgggaacgca ccaggaatcg cgacagccgg atccttctcc gttggtccag
    22141 aggccgtctg catcaaaatg aacccggggg taccatgaaa cccccaacgg ttcgcctctt
    22201 gacgatcctg aaatatcgac tccaggtggc gccgttcgtc gaaacacttt ccgaaggtga
    22261 cgggatctaa tccaatggat tttgcgaatc ccttgaatgc gccggaatcc aatcgaccgc
    22321 cttcattgaa caaccgatca tgcatggccc aataacggcc ttgcgcgccg gcacaccgtg
    22381 cagccaccgc tgcttcgact ccgactccgc gatccgctct cggatagtcg cgatacacga
    22441 atttgacttt cccggtgtcg atgtatttgg cctgcaattg cggcagggtc tccttgaaga
    22501 acttcaagca atagccgcag gtaaaatcgg aatactcgat caacgtgatc ggcgcgtctg
    22561 cacggcccct cacccgcccg tcgtcggtaa tcgatccggc tccgacatcg ttcgactgcc
    22621 atagggcgag gcccagcagg aacaccaccg ccgagcgata caggactccg gttctcatca
    22681 tgtccgtgcg gcgcgcttgg cattcatacg ctcaagcagg ccgaccatca gaagtggcca
    22741 cacaaccgtc gcgtcgctca ggacttcggc aaaccggccg ccctcttccg gaggaacgaa
    22801 tttcccccag gagattcctt cctgataggt gcaaccgctc aagccacccc agtaatccgg
    22861 ttcaggacag atccgtaccc cgtattgaaa ccgcggcggt cgaacgttga gccccaaacg
    22921 catgttgctg atctcaatgt acggcccgac ttgttgggac caattgcgcg gcacaccgcc
    22981 tccgatagta aagatgccga gccgtttggc cgacacgata tgattggtgt agctattcag
    23041 atcctgatag ggattgaacg acggatggga tttattgatc gctcgaagta tgtcgatgtc
    23101 gctgccaccc ttcgtctgtg cccgcgcctg atcaagcacc cgccccatcg cccaggttcc
    23161 gacatccaag cccatctccg aatcggtaaa ggcggggata tacaccggaa ccttcttcag
    23221 aaaggcgctt ttgagaatac cggcgccatc gaattcttcg gccagagtct tgcccagttc
    23281 acgcgtgagg atttcggatg agagcgactg atcttgattg attcgcttca tcgtttgggc
    23341 gaccacatat tcgacgtaat tgaggtttga ttccatctcc aacgtgtcat agacacggtt
    23401 atatcccttc ttaaacaact cctcgtcggt catcgacgga tgatgccggt aatgggtctt
    23461 gccgacagac tcgctcagcc cgtgagcaat caatgcgccg gtcgagacga cacattgcac
    23521 catgccgtga tcgatcatcg tgctgatgat ttttcccatc ttcgcgatcg tcatcgcgcc
    23581 agagagcgtc atcacgaccg agcaatcggg atcatcgatc atggcagaca atacttcgta
    23641 cgcctcgcct aaacgacggc cgccgaacgc ggtcttgcgc atggcgacca agagatcgtc
    23701 gaatgatccg attttctcgg gatcgagggg ctcgagggct tcgagtccat ccttggcccc
    23761 atcacgaaat tcacgcgcgc ccataggaga gtcttcccgt accagagttg aacgattaag
    23821 aaaggcgcat ccgttataca caaccgacgc tagaggcgtc aacgaagttt gggtggagaa
    23881 caggatgccg cgcgcgaagg ggcagctgtt cagaaaaacg aaacctgacg aagtttttct
    23941 gaattagtta tagcccctgc gagcgcctca acacaaagtg gaggctccgg ccaaacagcc
    24001 tgaatgcgtg atgacagccc acgaaaaact ttacccgtcg ctggcaagtc gacgcacgag
    24061 caaggcaatc gagaaagatt tttccggagg tgtttatccg ccggcaccag tctgataaag
    24121 cggtaacgag ccggcggccc acaggaacaa agaaatgaag gagttcagag tcgagcggct
    24181 cgccgcctaa gacggctccc gccaagcaag ctggatgtgg tggtcccaac gggattcgaa
    24241 cccgtgttta agccttgaga gggctccgtc ctaggccagg ctagacgatg ggaccagctc
    24301 aggtgccttg aaaagaaatg cgacagtacc atagggaatg gcggattatc aattcctggc
    24361 gcccggtgga ccgcttctcc ttggtgattc gggagcaata aaagcgactt gacagtacac
    24421 gtcgcttggc ctacctttcg caaatccttg gtacggtctt cgggacattc gccgggcctc
    24481 tacaacatag gtcttgatcg tttagcttgg ataaaaattc cgtctccatg aacaactatt
    24541 ttcagaccga ttccgacatg atggatctcg acgatgcccc ggaattggtc gtcgaagaag
    24601 aaaagcccga acagggtctg gaactgcaca tcggctcgaa tgtgtttcgc acgacgaacg
    24661 gtgtcattaa attgcaaggc aaggaacaaa tcgtactgga agtccagccc gatcccgcga
    24721 cactactgtt gacgatggat ttctacgacg agcacggcca aagaatcggg cacctcagac
    24781 gcaatacgct atcggcaacc ggatccagcc gcttcgccgt ctcgatgaac accacctctg
    24841 atgcgacgct cgacgacccc tttacggtga cgatttctga ccgaatgacc ggcgggacgg
    24901 tcattgaggt atatctcttc caacggcgga aaatccgagt gaccagcggc aattttcaca
    24961 ctcataaagg cgatctcgtt tcgatcagcc cccattactg ccggcttgga acaggactga
    25021 cgatgttcgg agacgtcgtt gaaagccggg ggggcacggc tgtcatcgga taagtgatct
    25081 tccaaaatgc ccccgaaaat gcaggcttct tctcttgccc tctctccgcc cggttcccag
    25141 aaatagaaag aaaaccggat ccttttgaca gcctcaaata ctcccgtaga ataaggctat
    25201 gtcagatatc gcaaaaccca gtattcaagc gttcttggtt tgtgatcagg tcatcgaaga
    25261 cagcgtgacc aagaagaaaa gcctgatcgg cctgttcaca caccttcaag ccatcagctt
    25321 ccccttccaa catcagcaaa tggggctcta cttctgcctc accgacgcag aaggaaccta
    25381 ccagtttgac atcgacttga tctatctcaa cagcgagcaa ctcgtctgtc gagccaatct
    25441 ccccaacatc gttatcggtg atcgactgca gatttcagat ttcggcatta acattccctc
    25501 gttgattttc ccggcccctg gacgctacga gtttcgattg cggatggaag gacatctcat
    25561 cgcccagaaa gatttcaacg tcatgcagat gccgacccat caagccgcat aaccgcgcag
    25621 gctccctctg gcaccctcta tcccctagca gcccttctcc cctgtgcggt tcttatggta
    25681 gaactgcccg cgaatgatca acgagcggat gattgtcatt cgcctgatgg ttaaaggaac
    25741 atcgtggctg aaggaacgaa cgcctccaat tttatccgcg atatcgtggc tgccgaccgt
    25801 gcggcaggca agcatggggg ccgcgtcgtc acgcgctttc cgccggagcc caacggctac
    25861 cttcacatcg gccatgcgaa atcgatctgc ctgaacttcg gtctggccca agagacaccc
    25921 ggcggaatct gccaccttcg gttcgacgat accaatccca ccacggaaga ccctgaatac
    25981 gtccaatcga tccaggatga tgtccgatgg ctcgggtttg attggcagga caagctcttt
    26041 ttcgcatcgg actacttcga acagctctac gagtttgctg aaggattaat ccggaaggga
    26101 gccgcctatg tcgacagtct cacagccgag gacatacgca cctatcgcgg aaccttgacc
    26161 gagcccggca gaaacagccc gtatcgcgag cgcttcgtcg aggaaaatct gtctctcttc
    26221 cgggaaatgc gcgccggcaa atttcctgac gggacacatg ttctgcgcgc caagatcaat
    26281 atggcgtccc ccaatattaa cctgcgtgat ccggtgctct accgcatcag acatgccgct
    26341 cactaccgca cgggaactgc atggtgcatc taccctgcct acgactatgc ccatccactc
    26401 tctgacgcca tcgaaggcat tacccattca atctgcacgc tggagtttga agatcatcgt
    26461 ccactgtacg attgggtgat cgaacaagct cagacgccgc accgacccca gcagatcgag
    26521 tttgcccgct tgaacatcag ctatgccgtc atgagcaaac ggaagctgct ggagttggtc
    26581 gagaaaaatc tcgtggccgg gtgggatgat cctcgcctcc ccacaatcaa ggggctgcgc
    26641 cgtcgaggat atacgccgga ggccattcgt gcattttgcg agcacattgg cgtggcaaaa
    26701 cgagatgccg tcgtcgagat gcaactcctt gagcatttta tccgggaaga tttgaacaag
    26761 cggtcgcttc gtgtcatggc agtcttgaaa cccctgaagg tcgtcctcga taactatccg
    26821 gaaggggccg tcgaagaatt ggacgcggtc aacaacccgg aggatcattc tgccggcacg
    26881 agaaaagttc ctttctcgcg caccctgtat atcgagcggg atgattttct cgaagacccg
    26941 cccaagcaat tctttcggct tgccccgggg cgggaggtcc ggctccgtta tgcctatatc
    27001 atcaaatgtg tggaggtgac aaaagatcct caaaccggcg aaatcatcga gttacattgc
    27061 acctacgatc catcgaccaa gagcggagcc gctgaagccc aacggaaagt taaagccacc
    27121 attcattggg tatcgacatc tcatgcactg caagcggaag ttcgtcttta caatccccta
    27181 ctgatcgccg atctctctaa agttccgaca gaccacgatt ggacgacgta tctcaatccg
    27241 caatccttag aacgaatcac cggatgccaa gtggaaccga gccttagggg cgcaccgccc
    27301 ggctccagat tccagtttga acgtctcggc tacttctgta ccgaccttga ttccacgaac
    27361 gactctcccg tcttcaaccg caccgtttcg ctcaaagacg cttgggctaa gctcgataag
    27421 agacaacctt cccggtaatc cctctttgcc tgcaacctcc tcatccaacg cacagaaact
    27481 cttccctgtt tacgcgacct tgcactccct ttacagactg ggcatccctg ctaggctaga
    27541 agggtacgcg tagaaacatg cgacaggggc caatggacaa agatgccaac ttttgacacg
    27601 ctgtgccgac atattgagaa gacgagccgt tggacctgca tgctggccct gctggcaatc
    27661 actggccctc gggcggttga tgccggacag gctatgacgg acgagagtgc cacggaagcc
    27721 gtgaaacgaa ccatcaccga tctcatctat gtgctcgacg acgataccct gaagcaaccg
    27781 gaacaatccg aaaatcgccg ccgcgaaatt gaagaaatca tccggcagcg ggcggactac
    27841 gaagagatgg ccaaacgggc gctgggcgcc ccctggtcaa ccctcttgaa aagagaacag
    27901 caggaattcg tcagcctctt cgtccaattg ctccgcgata ccttcgccgg gcggattacg
    27961 gaacaccacg acgaacaagt tgcctatctt ggagagtatc gagaagaaga tgtctacgcc
    28021 gaagttcaca cccgcttgaa agggcgaaag attgatacgc tgatcgatta ccgaatgatc
    28081 aaccgcgggg gcgattggcg tgtctatgac gtggtcatcg atggagccag catcgtgagc
    28141 aactaccgtt cgcaattcac gagcatcatc cgcgaagtat cttatgtcgg tctggtcaca
    28201 aggatgaaac aaaaggccat tgccgtgaag gtcttcgaaa ggtactccgc gccataacgc
    28261 agcacttgcc tagcttttcc gtccagcaga atttcgaggt ttcctttacc gaatcaggcg
    28321 gtttgttaca ctggcagaga aacggcgtcc aggccgatgc ccctcacgac agcagaagga
    28381 gccgaccatg atcgaatgtc gcgaacaccc acgcattccg gttgacctgc aggtatattt
    28441 ttcgacgaca ggcaatacgt cgatccgcga gggaacgatg ttcgatcttt ccgcaggagg
    28501 ctgtgctgtt gccagcatga ccaccgtgca gcccggctcc gcagtcagaa tcctcattcg
    28561 cgccactgat ctaggctctc cgatcacgat cgaatccgcc gctgtacggt ggagcagcca
    28621 cggtgaattc ggagtcgaat tcgtcggcct ttcggaaatc gatcagagtc gcctgcaacg
    28681 cctgttagaa atcagaacgt caccccacat ccgtcccagt tgatcgctgg caagagccgc
    28741 gcatccgcgt ggtagaatgt cggcatgaac tgcccacttt gcggtcaacc aacaacctgg
    28801 aatggcaatc cttggcgtcc cttctgctct gaacggtgtc aactcacgga cctcggcacc
    28861 tgggctgcgg aggagtatcg cgtgcctggc cccagcctga cgatggaact accctccgat
    28921 gagaaccatg aaaccgaaga agaaacgggc gggaaacagt gaggttaagc cggcaatccc
    28981 agtgcaggaa actctagcca cttgaacagc gtcacagcag atcccactaa atccttagac
    29041 tgccgacgaa tagagacttt gatgggccgc ctgctacttc aggtcgccct cctgtagaaa
    29101 cctcgttgac gcctatcgcg cctgtttcat agttggcaag ttccagcgat gttgctaagg
    29161 tcagcccttg cccgcaacct agacaaggag acgcgccatg ctcccaacaa aagggtttgc
    29221 cgcaatgaca gccaaggctg ccctgcagcc tttttcattc gcgcggcgcg aaatagggcc
    29281 tcacgatgta ttgatcacga tatcgcactg cggcatctgc cattctgaca tccaccaggc
    29341 acgcgatgaa tggggcggat cgattttccc catggttccc ggccatgaaa tcgtgggtgc
    29401 gatcgcaggg gcgggaaatg ccgttaagaa gtttcgtgtc ggtgacgtag caggcgtggg
    29461 ttgcttcgtg gattcctgcc gatcgtgctc agcctgccgt gaaggtctgg aacagtactg
    29521 cgaagccggc atgctcctga cctacagcgc acgagacaag aatggccaga tcacgcaagg
    29581 cgggtactcc tcccagatcg tcgtcgatga aaattatgta ctacgaattc ccaaaacgct
    29641 ttcgccggct ggcgctgccc cgcttctctg tgccgggatt acgacctatt ccccgctgcg
    29701 ccattgggga gtaggcaagt accacaaatt agctgtagtg ggtctaggtg gactcggcca
    29761 tatggccgtc aaaatcgcga aggcccttgg cactgaggtc actgtcttca gcacatcgga
    29821 atccaagcgg aaggacgcaa taagattagg cgcaacagat tttgtggtga cgacgaaccc
    29881 caacgccttt gcacagccgc agcgtcggtt tgatttcatt ctcgatacag tgtcggctcc
    29941 gcacaactac aacagttatg tgaatctgct gaagactgac ggcacgatga tcctcgttgg
    30001 tgcaccggac aaaccgactc cgctcgaacc atttccgttg atcacgcatc ggcgccgaat
    30061 tgccggctcc gtgatcggcg gcgtcaggga aacgcaggat atgctcgatt tctgcgctga
    30121 gcatgagatt gaatcagatg tcgaagtgat tccgatccag caggtcaacg aggcctacga
    30181 gcgagttctc aagggcgacg tgcgcttccg gttcgtaatc gatttgatgt cgctgaaata
    30241 gggacttcgt cgaatagagc gtcgattgga aagtggctgg gggactagga atcgaaccta
    30301 ggtagccggc tccaaaggcc ggcgtcctac cgctagacga tcccccagcg cggtacggaa
    30361 gcaacgcgta gatctggatt aggtcggcgc gggaaagatt attggctggg ggactaggaa
    30421 tcgaacctag gtagtcagat ccagagactg acgtcctacc gctagacgat cccccaaccg
    30481 acgctcacag taatataagg gtctcggccc cgtcaagatc gacgcgggtt tgatcgacac
    30541 gtctcttaga ctccggatgc ccgttcaatt cccttgcgga gccgaaatag cgtgcgatct
    30601 cgccccaata cttccatcac ttcgaacagc cctgggctgg cagcgcgacc ggtgagtgcc
    30661 acacgaaccg gctgggcaag ttggcccatc ttcattcctt cttcctcgac caattgcttg
    30721 aagctttctt cccactcctg cttggagaaa gtgggaaagg cttcgaaccg cgcgagcaac
    30781 ttccccagaa tcggagcgat cgccggcgtc aagaacttct tgactgattc ttcgtcgaac
    30841 gcaacggttt ggccgaaata gggggtgacc cacatcacca tatcggtcag cgtcttggtc
    30901 cgttccttca ccaggacgac aagctgtccc agccattggt tcgagaccgc cctcacctct
    30961 tccttgaggc ctgcgttttc cagcagcgga acaagctccc ctgcgatctg gctcggggga
    31021 gtcgttttaa tgtattcggc attcatccac aggagtttct ctggattgaa caccgccgcg
    31081 gacttttgca cgtgatccca ggaaaatttt tcgatcaatt cctgcctggt gaagatctct
    31141 tgatcgccgt gcgaccagcc aagccggacc aagtagttca ccatcgcatc ggacagatac
    31201 cccatctctt tataggccat gatcgacgtg gcgccatgac gtttggacag tcgtgccttg
    31261 tcggatccaa gaatcatcgg tagatgcccg aaacgcggga cgggaaaccc cagcgcctgg
    31321 aagattggaa cttgccgagg cgtgttggtc aggtgatcgt ctcctcgcac gacgtgtgtg
    31381 atgttcatca acgcatcatc gaccacgacc gaaaagttgt aggtcggata accgttggat
    31441 cgctggataa tgaggtcatc aaggacggtg ttatcgaaga cgatctttcc tttgatcagg
    31501 tcgtcgacga cagtctgccc ttcctgcggc gccttgaaac gcaaggcagc ttcgtctgaa
    31561 gggttggtaa tgccgaggtt tcgacagcgc ccatcgtaac ggggcgagag ccctttggct
    31621 tccgcttctt tacggcgagc ctccaattct tctgctttac agacgcacca gtaggcctgt
    31681 cccctctcca aaagatgcat cgcgtggctg cgatagaggt ccatgcgttc ggtctggcga
    31741 aatgggcctt catcccaatc gagcccaacc catttcatgc cttcgatgat ggcctgaatc
    31801 gattcatcgg tcgagcggct ctggtccgta tcctcaatac ggaggacgaa cacaccctgt
    31861 tgctggcgtg cgaagagcca attaaacaga gctgtgcgga ctccaccgat atgaagaaag
    31921 cccgtcgggc taggcgcgaa tcggactctg acctgactca ttcgctcgtc ctaagttctt
    31981 gtggaaaggc cggtatctat aacatgcagc gaaaagtatt gtacagaatc cgtcgtcctt
    32041 ccctattgct tgcgcttctg ataagagatc cgtacaagat tatcatggca gaattcatcc
    32101 aactggctac ggtcctgtca accaccaatc tgacgccggg cgttcgccag ctgatcctgc
    32161 taccaaaaga gcggcacatc tcctttcagc ctggccagtg ggtctccatg aagttgccgg
    32221 tggggagaaa accgccgctc aatcgcgcct attcgatggc ggctcccgcc acgccatcgg
    32281 gcgaactcac cttagtcttc gatcatgtcg taggtggatt gggatcggaa tatctctgtc
    32341 gactcaaagc gggtgatgag gttgcggtct ccggtcccta tggcaatttt acacttcctt
    32401 cacccctcga ccgagagctg gtgttcgtcg cccgctatac ggggctcgtg cccatccgct
    32461 gcatgctcaa acaactttac gctcagcggc aaacaggctc gatcctgctg atcgccgtcg
    32521 ctccggggga agacgagctg ctctttcatc aggagttgct caccttggcc gtcacccatg
    32581 cgggcttccg ctatcttccg ttggttgcag ccggagggga gcaacaaggc gtcgatctcg
    32641 cactgtcaat gctcagcccg ctgattgacg gcgcaccaaa agtgattccg atgctgtgcg
    32701 gaacgaagca cttcgtgcgc ccgttgcgag cctatttcac aggggcaggc tacggtcgaa
    32761 aggaggtaaa gaccgaaacc tacgactagt gcccttctct gaccagattc ttattcaccg
    32821 ccggaatctc caccacaata tctttcgtgc cgttcgggac gcattgacag ccaagccgtg
    32881 attgcagtgt cgtcatgggt gcttcatcaa gctgatcgaa ctcgtcatcg gtaccctcgc
    32941 tgcaactctc aagtccctgc tttacaatga catggcaggt cgagcaagcg cagacgcccc
    33001 cgcagacatg ctccaggtca accccgttac ccattgcgac gtccaggatg ctcccaggaa
    33061 gcccggtctg gccataggga atcctgccag gatccacttc aaccttggtt acattcccat
    33121 ccggagcaat atatgtgatc gtataggatt tcttgggaag ctcgtatgca gccttttcaa
    33181 tataggggtt tgtcccgccc atgaactcgg tcctttctcg cgctcagcct gattggcctt
    33241 accgcaactg ttgacagccc tgaaaccagc gtggtattct taatccgacc taaatgatcg
    33301 gagatcatta tagtctccga ccctatggat cggcaatagc catgttgaag atttcaaaga
    33361 aagccgacta cgctctgatg gcgctccagc acatcgcctc ggtccaattt ggcgacgtga
    33421 ccccgggccg cgtcgtcaat accaaagaga tcgctgaaga atacaacatt cccctcgaac
    33481 tgctggcgaa ggttcttcag gcccttgcga aaaacggcat gattgagagc cataacggcc
    33541 ccaagggcgg ctacctgctg gccagaagcg ccaggcagat tacgatctcg caggtcttgg
    33601 aaagcatcga gggaccactc ggcatcaccg attgctcgca cgaaaaagac ggtgaatttt
    33661 gcatgcagcg ggaacattgc aacatccgca caccactgct caaggtacaa gacagcattt
    33721 atcagttact caacaatatg accctgcaag acatgctggg tggaaccccg ctgattacga
    33781 ttcagtcgcc gacagcacaa ggagttgaac gatgaagctt cccattttcc ttgataatca
    33841 ttccacgaca ccgatggatc ctcgagtgtt ggaaaccatg cttccctact ttgtcgaaaa
    33901 attcggcaat gccgccagcc ggaaccacgc gttcggctgg gccgcggaag aagcggtgga
    33961 aaacgcccgc aaacagatcg ccaagctgat caaggcggac ccgaaagaaa tcgtgttcac
    34021 cagcggagcc accgagtcag acaacctggc gctcaagggc gtcctggaaa tgtacaagga
    34081 gaagggcaac catatcatta cgtcgtccac ggaacatcgc gccgtgctcg atacagccaa
    34141 gtccatggag gccaagggac tcgccacggt cacctttctc ccggtcgata agcaggggat
    34201 ggtgaatccc gacgatgtca gaaatgccat cacgggcaag accgttctga tttccattat
    34261 gttggccaat aatgaaatcg gcacgatcaa tcccgtgcag gagatcggaa agatcgcgaa
    34321 ggaaaagggc attctcttcc attgcgacgc gacacaaggg gtcggcaaga tccctgtcga
    34381 tgtgcaaggc atgggcatcg acctcatgtc gttctcggcc cacaagatct acggaccgaa
    34441 aggcgtgggc gcgctctatg ttcggaagaa aaacccgcgg gtgcgtatcg ccccgcaaat
    34501 ggacggtggc ggtcatgagc gcggtatgcg ctccggtacg cttcctgttc cattgatcgt
    34561 cggattcggg aaagcctgtg agctctgcga gcaagagatg gccaaggaat ccgctcggct
    34621 gtcggccatg cgcgaccggc tccaagccgg aatcatggcg gccctggacg aatgttatct
    34681 gaacggccac cccaccagtc ggctgccggg caacctgaat atctcctttg cctatgtgga
    34741 gggggaatcg ttgctgatgg gcatgaagga tatcgccttg tcgtccggtt cggcctgtac
    34801 ctcggccacg ctggaaccgt cgtatgtcct ccgcgcctta ggggtcggga cagaactggc
    34861 acactcttcc atccgattcg gattaggccg gttcaacacg gacgatgaga ttgactatac
    34921 gatcaagaag gttattgaga ttgtcaccaa actgcgggag atgtctccgc tttacgaaat
    34981 ggcgaaagag ggtattgatc tgaaatccgt tcagtgggct gcacactgaa tcgcgcggga
    35041 aacgaaggag gaagtcatgg cctatagcga taaagtcgtc gatcatttta ataatccccg
    35101 gaacatgggt agcttcaaga aggacgagga tggggtcggc tccctcgacg cgatcacctg
    35161 gtacggcccc gacgaaaagc cggcacgcga ggagctcgac accaacaagg acggcaaacc
    35221 cgacgtgcgg cgcagctacg aggcgggcgc gctcgcgcgc gaagagcagg acgcggacaa
    35281 ggacgggcgc ttcgagctca ccacgttctt cgcgaacggc gcgcccgcgc gctcggaggc
    35341 cgatgcgaac ggcgacggca agcccgacgt gctcgtgacc tacgccggcg gaaagcgcgc
    35401 ggagcagcag gaggaccgcg acctgaacgg cgcgatcgac gccgtcaccc gcttcggccc
    35461 cgacgacaag cccgcgcaag tccaggagga cagcgacggc gacgggcgcc tcgagaccac
    35521 gagctactac gagagcggcg ccgtcgtgcg cagcgagcgc gaccgcaacg gcgacggcaa
    35581 gccggacctg cgcgtcacct atcagggcgg caagcctgcg agcaccgagc aggacgacga
    35641 ctacgacggc agcttcgaga agcgcgtggc ggcgtcgagc ggaggcacgc aggagcagag
    35701 cgaggaccgc aacggcgacg gcaagcccga cctgcgcgtc gtgttcgacg ccaagggcca
    35761 gatcacgcgg caggaggagg accgcaactt cgacggcaag ccggacgtga tctcggagtt
    35821 ctcgggcggc gtgctgcggc tcgtgcgcga ggactcgaag ggccacggct gcttcgacgt
    35881 ggagcagcgg ctcgcggccg acggcaagac ggtcgaggag caggcggtcg acgagaacgg
    35941 cgactgcaag ctcgacctgt ggtcgcactt cgcgaacggc gcgccggtct ggcaggcgca
    36001 ggaccagcgc ggcgcgggat tcgcgaccgt gctcacccgc ttcgacggga gcggcgcggc
    36061 gacctctcag gagctgatca gcgagggcaa gcgcgcgccg gacacgaaga tcttcctcgc
    36121 gcccggcggc gcggtcgcgg cgcagtgcct cgacagcgac ggcaaccgca ccctcgacgt
    36181 gcgctaccgc gtcgagggcg gagcggtggt cgaggggctc gtcgactcca acaaggacgg
    36241 caaggccgac gtgcggcagg tctggtccgg cggcgcgatc gagcacgcgg agatcgacac
    36301 gaacggcgac gggcgcgccg acgtcgtgca gtatttctcg ggccagaacg tcgtccgcca
    36361 gtgcgaggac gagcagtacg acggcagcgt cgatcgctgc ttcgaaggcc agaaggtcgt
    36421 accggtgagc ggcgtgaagg atctctcggc gaagctgccc gccctcgatt gcggcggctt
    36481 cgatccgttc tggaaacgct gaacgtacgt tcggtgccgc cctgagaaag cttgccccgc
    36541 cgcccccgcg tgcgcgtagg atgcggccgc tcaggagccc ggatgcgcgc cgctctcctc
    36601 acgacgctgg tcgttttgct ctgcctcgtc gtgtacgccg agtaccggcg tagccctccg
    36661 ccggagaacg ccgcggatgc cgtcgcggag cgcacccaga gcgcgcccgc cggagcgccc
    36721 ggcgggctgc tggagcccgc tcctcgcacc gatcccgcgg cagcgtcgat tccgagcgac
    36781 gcgagcaccc gttacgagac cgctgccgcc gcgcagccga ccccgctgcg gcgccacctg
    36841 gtgcgccccg gcgacacgct cgcctcactg gccgcacgct attacggaga tccggcgcgc
    36901 ggccaagaga tctacgaggc gaatcgcgat cgcatccgcg acccggagcg tctccacgac
    36961 ggtcaggagc tcctgattcc ctgaggcggg ccgcgcgccg ccgcacagtg ccgtcgcgcc
    37021 gccc
//
LOCUS       DATIFR010000258         1446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_760758, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000258 DATIFR010000000
VERSION     DATIFR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760758"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="rplF"
                     /locus_tag="VLD60_15030"
     CDS             <1..471
                     /gene="rplF"
                     /locus_tag="VLD60_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSB46324.1"
                     /translation="VVSVKGPLGKLDWTLTPGIGVTVDKGQVIVTRSSEDRNIRAMHG
                     LVRAELNNMVHGVTKGYERNLEITGVGYKTAVQGRTMSFNVGYINPVVYAVPTGIDVK
                     VDKQTLINIKGADKRLVGQVAANLRAIKPPDVYKQKGVRYAGEVLRKKEGKTGK"
     gene            480..845
                     /gene="rplR"
                     /locus_tag="VLD60_15035"
     CDS             480..845
                     /gene="rplR"
                     /locus_tag="VLD60_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSB46325.1"
                     /translation="MNSAEKVRHLARRQQRVRQRVFGTNERPRLNVYRSSGHIYAQII
                     NDLKGTTLAAASSLDKSLRTTLKSTSSIVAAKAVGKLLAERAKSAKIQAVVFDRGGRL
                     YHGRIKALADASREGGLQF"
     gene            906..1415
                     /gene="rpsE"
                     /locus_tag="VLD60_15040"
     CDS             906..1415
                     /gene="rpsE"
                     /locus_tag="VLD60_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSB46326.1"
                     /translation="MRVNPDELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGQGW
                     VGIGKGKAAEVPVAIAKAVQQAKKNLVHVPLKSGTIPHEVHGLFGGEHVLLKPAAEGT
                     GIIAGGAVRAVVELVGAHNIISKTLGRGNPFNTVRATLDGLTQLRNPEEVMRLRRETA
                     QGLQSGVMA"
BASE COUNT          350 a          345 c          480 g          271 t
ORIGIN      
        1 gttgtgtccg tgaagggccc gctggggaaa ctcgactgga cgttgacgcc gggcatcgga
       61 gtgacggttg ataaagggca ggtcatcgtg acgagatcca gcgaggaccg caacattcgg
      121 gctatgcatg ggctggttcg cgcggaactc aacaacatgg tgcatggcgt cacgaagggc
      181 tatgaacgca atctggaaat caccggtgtc ggttataaga cggccgttca ggggcgcacg
      241 atgagtttta acgtcggcta catcaatccg gtggtctatg ccgtgccgac cggcatcgat
      301 gtcaaggtcg acaagcagac cttgatcaac atcaaggggg ctgataaacg gcttgtgggt
      361 caggtggccg ccaatctgcg ggccattaag ccgccggacg tctataagca aaagggtgtc
      421 cggtatgcgg gagaagtttt gaggaagaaa gaaggaaaga ccggaaagta ggttgatcta
      481 tgaatagcgc agaaaaagta cgccatctag cacgacgtca gcagcgagtt cgccagcggg
      541 tgtttgggac caacgagcgc cctcggctca atgtctaccg tagcagcgga catatttatg
      601 cacagatcat caatgatctg aaagggacaa cgctggctgc cgcgtcctcc ctcgacaagt
      661 ccttgcggac gacgctgaaa tcgacgagca gtattgtcgc ggcaaaggcc gtcggaaaat
      721 tgttagcaga gcgggccaaa agcgccaaga ttcaggcagt ggtgttcgat cgtggtggcc
      781 ggttgtacca cggtcggatc aaggccttgg cggatgcctc acgtgagggc ggcctgcagt
      841 tctagggcga tgactctcat tttcccgatc gaggaaatga gaggaaccaa gaggagacac
      901 gaggcgtgcg agttaatcca gatgaactga gcctgaaaga caaagtcgtc ttcatcaatc
      961 gtgtggccaa ggtcgtaaag ggcgggaagc gattcaactt ctgcgcgctc gtggtcgtgg
     1021 gagatggaca gggatgggtc gggatcggga agggcaaggc ggccgaggtg ccggttgcga
     1081 tcgcgaaagc ggtgcaacag gccaaaaaga acctggttca tgtccccttg aagtccggca
     1141 cgattccgca cgaagtccac ggactgtttg gcggcgagca cgtcctgctc aagccggctg
     1201 cagaagggac aggaatcatc gcgggcggtg ccgtgcgggc cgtcgtggaa ttggtcggtg
     1261 cgcacaatat tatttctaag acgctgggac ggggcaatcc gttcaacacc gttcgggcga
     1321 cgctcgacgg tctcacgcag ctcagaaatc ctgaggaagt gatgcggttg cgtcgtgaga
     1381 cagcgcaagg attacagagc ggagtgatgg cgtaatggct acggcaaaaa ctgcgaaaag
     1441 ttcggc
//
LOCUS       DATIFR010000259         3583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2104025, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000259 DATIFR010000000
VERSION     DATIFR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104025"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VLD60_15045"
     CDS             <1..833
                     /locus_tag="VLD60_15045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSB46327.1"
                     /translation="LQKLIAGAGLASRRKAEALIAAGRVTVNGKVVTELGTKADPSKD
                     HVKVDGKHLSQAQPFVYLLLNKPKNVMSTLDDPGGRTTVKDYLRGVSVRVFPVGRLDF
                     DSEGLMLLTNNGDLAQALLHPRYHVPKTYLIKVKGILTEEEIRQLERGVTLEDGMTGP
                     ATVKKIKKAEMNSWLEITIGEGRKHQVRRMLESVGHPVIKLMRVKMGPLSLGDLASGE
                     FRFLTDREANALRELVEQRREAVESGAPVDRPKRPVRREGWARPIRTKKYVGKKARVA
                     "
     gene            830..2593
                     /gene="aspS"
                     /locus_tag="VLD60_15050"
     CDS             830..2593
                     /gene="aspS"
                     /locus_tag="VLD60_15050"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSB46328.1"
                     /translation="MKVRTHRCGELNKTHVGQTVVLNGWVQRRRDHGTVMFVDLRDRT
                     GITQVVFNAERNAAVHQAAHVLRSECVVAVTGQVMARPEESRNSHLPTGDIEIFVDAV
                     EVLNEAKTPPFLIEDDAEVTESIRLKYRYLDLRRPKMQRLMGIRHHVMQATRGFLNAE
                     DFLEIETPILTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQVLMVSGMDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDREHVMNLTERMIVTVFREAGGVQLPTPFPRM
                     TYAEAMGRYGSDKPDLRFDMPLHDVTNFAAASEFKVFKEAATKGGIVKALIVKGGGTI
                     ARSRIDALGETAKSFGAKGLAWLKITADGQLESVIAKFLDAKAFAAALPEAKPGDLIL
                     FGADKPAVVHDVLGRIRLLLGEELNLIDKAAWRPLWVTEFPLLDYSPEEKRYIFMHNP
                     FAAPMDEDLALLDSEPLRVRAKAYDMVLNGSEIGGGSIRNHRSEIQLKILDLLGIGKD
                     QAHAKFGFLLDALEYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQKAQCPLTD
                     APSAVSQDQLKELRIKLDLVE"
     gene            2606..>3583
                     /gene="aroC"
                     /locus_tag="VLD60_15055"
     CDS             2606..>3583
                     /gene="aroC"
                     /locus_tag="VLD60_15055"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSB46329.1"
                     /translation="MAGNTFGRVFTITSFGESHGPAIGCVVDGCPPGLTLSAADIQKD
                     LDRRKPGTSRHVTQRQESDTVEILSGVFEGRTTGTPIALLIRNEDARSKDYGNLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRASARETAVRVAAAAIARKWLNEKYGVVIRGYLSQL
                     GPHPVPFKSWDAVSGNPFFAADSDMVATLESFMDELRKSGDSVGARITTIAEHVPVGW
                     GAPVYAKLDSDLAAAMMSINAVKAVEIGAGFKAVTQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDIIVTIGIKPTSSIRVPRRSIDKQGNPTTIETNGRHDPCVGI"
BASE COUNT          769 a          960 c         1143 g          711 t
ORIGIN      
        1 ggcttcaaaa actcattgcc ggagcgggcc tggcgtcgcg ccgcaaagcg gaagctctga
       61 ttgcggccgg ccgtgtgacg gtaaatggca aggttgtgac ggaactgggt acaaaggctg
      121 atccgtcaaa agatcatgtc aaggtcgacg gaaagcattt gagccaggcg cagccgttcg
      181 tgtacttgct gctgaacaag ccgaagaacg tcatgtccac gctggacgat ccaggcgggc
      241 ggacgacggt gaaagattat ttgcgcgggg tctctgtgcg ggtttttcca gtcggccgct
      301 tggacttcga cagcgaaggg ctgatgctcc tgacgaacaa cggcgatctc gcgcaggcgc
      361 tcttgcatcc tcgctaccat gtgccgaaaa cgtatctgat caaggtcaaa ggcatcttga
      421 cagaggagga gatcaggcag ctggagcgag gggtgacgct cgaagacggc atgaccggtc
      481 cggcgacggt gaagaagatc aagaaggccg aaatgaattc ctggctggaa attaccatcg
      541 gtgaagggcg aaagcatcaa gtcaggcgca tgttggagtc ggtcggccat ccggtcatca
      601 agctgatgcg ggtcaagatg ggaccgctgt ccctgggcga tctcgcttca ggagaattcc
      661 gttttctgac cgatcgcgag gcgaacgcgc tgcgggaatt ggttgagcag cggcgcgagg
      721 cggtcgaaag cggggctccg gtggaccggc cgaaacgacc cgtcagacgt gaaggttggg
      781 ccaggccgat ccgaacgaag aagtatgttg ggaagaaagc gagagtggca tgaaggttag
      841 gacacaccga tgcggggaat tgaacaagac gcatgtgggg cagaccgtcg tcctgaacgg
      901 gtgggttcaa cgtcgccgag atcacggcac ggtcatgttc gttgatctgc gggatcggac
      961 ggggatcacg caggtcgttt tcaatgcgga acgcaatgcc gcggtgcatc aggcggccca
     1021 tgtgttgcga agtgaatgtg tcgtcgcggt gacaggccaa gtcatggcca ggccggagga
     1081 gtcccgaaac tcacatcttc cgacagggga catcgaaatc tttgttgatg ccgttgaggt
     1141 tctgaacgag gccaagacgc cgccgtttct gatcgaagac gatgccgagg tcacggagtc
     1201 gatccggctc aagtatcgat atttggatct ccgtcgcccg aaaatgcaac ggctgatggg
     1261 catccggcat cacgtcatgc aggccacgcg cggatttctc aatgccgagg attttctcga
     1321 aatcgaaacg ccgattctga cgaagagtac gccggaaggg gcgcgggact atctcgtgcc
     1381 gagccgggtg aatgccggac agttttttgc tcttccccag tcgccccagc tattcaagca
     1441 agtgctgatg gtcagcggga tggatcgata ttaccaaata gctcgctgtt tccgcgatga
     1501 agacttacgc aacgatcgcc agccggaatt tacgcagatc gatctggaaa tgtcgtttgt
     1561 cgatcgcgaa cacgtcatga acttgacgga gcgcatgatc gtgaccgtct tccgggaggc
     1621 cggcggggta caactcccga cgccgttccc gcggatgacc tatgcggaag cgatggggcg
     1681 ctacggatcg gataagccgg atttgcgatt cgacatgcct ctccacgatg tcacgaactt
     1741 tgccgcagct agcgaattca aggtcttcaa agaggcggcc acgaaaggcg gcatcgtgaa
     1801 ggcgctgatt gtgaaaggtg gcggaacgat cgcgcgcagc cgtatcgatg ccctggggga
     1861 aacagccaag agttttggag caaaggggtt ggcctggctg aagattaccg ctgatggtca
     1921 acttgagtcc gtcatagcca agtttctgga tgccaaggcg tttgccgccg cgttgccgga
     1981 ggccaagcct ggcgatctta tactcttcgg ggcggacaaa ccggctgtcg ttcatgatgt
     2041 gctgggccgc atcaggctgt tgctcggcga ggagctgaat cttatcgata aggctgcgtg
     2101 gagaccgctc tgggtgaccg agttcccgct tctcgactat tcgccggaag agaagcgcta
     2161 tatcttcatg cacaacccct tcgccgcgcc catggacgag gatcttgccc tgctggattc
     2221 cgaaccgttg agagtgaggg cgaaggcgta cgacatggtc ttgaatggca gcgaaatcgg
     2281 aggagggagc atccgcaacc atcggagcga gatccagctc aagattttgg atctgctggg
     2341 catcgggaaa gatcaggccc acgccaagtt cgggttcctg ctcgacgcgc ttgagtatgg
     2401 cgcgccgcct cacggtggaa tcgctttcgg tctcgatcgt ttgatcatgc tgctgggggg
     2461 agccgactcg attcgcgatg ttattgcttt tcccaagact caaaaagccc aatgtccgct
     2521 gactgacgca ccgtcggctg tgagtcagga tcagctgaag gagctgcgca ttaagctcga
     2581 tctggtcgag tagggaggct cgtccatggc cggcaatacg tttggcaggg tcttcacgat
     2641 cacctcgttc ggcgaaagcc acggccctgc aatcggctgc gtggttgatg gctgtccccc
     2701 cggtctcaca ctctccgccg ctgacattca gaaagacctg gaccgtcgca agcctgggac
     2761 ctcccgccac gtcacacagc gacaggaatc cgataccgtc gaaattctct ccggcgtgtt
     2821 cgagggccga accaccggca cgccgatcgc cctgctcatt cgcaacgaag atgcgcgcag
     2881 caaggattac ggcaacctga tcgatacgtt ccgtcccgga cacgccgatt acacctattg
     2941 gcagaagtac gggatccgcg accatcgagg cggcggccgt gcctcggcgc gcgaaaccgc
     3001 cgtccgggtg gcggccgcgg ccattgccag gaagtggctc aatgaaaaat acggcgtggt
     3061 gattcgcggg tatctgagtc aactcgggcc tcatcccgtg ccgttcaaga gttgggatgc
     3121 agtgagcggc aacccgtttt ttgccgccga ctctgatatg gtggcaacac tcgagtcttt
     3181 catggatgag cttcggaaat ccggcgactc ggtcggcgcc aggattacaa cgattgccga
     3241 gcacgtgccg gtcgggtggg gcgcgccggt ctatgccaag ctggattccg atctggccgc
     3301 ggcgatgatg agcattaacg cggtgaaggc cgttgagatt ggtgccggtt ttaaggctgt
     3361 cacacagcgc ggttcagaac atggtgacga acttacgcct gaggggtttg tgacgaacca
     3421 cgccggcggc attctcggag gaatatcgac gggccaggat attatcgtga cgatcggcat
     3481 caagcccacc tcgagcatac gcgtgccgcg ccgttcgatc gataagcagg gcaatcccac
     3541 cactattgaa acgaacgggc gtcatgatcc ctgcgtcggc atc
//
LOCUS       DATIFR010000260         8576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_186522, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000260 DATIFR010000000
VERSION     DATIFR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186522"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VLD60_15060"
     CDS             <1..366
                     /locus_tag="VLD60_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46330.1"
                     /translation="GQTVEELDDRLIEAALARLDPLALGVAVGLVSGVGILLATAILL
                     LKGGEHVGQNLSLLRNYFPGFEVTWTGAAIGFLEVGLMGFGLGYLAAWLRNWEIEAYA
                     MFVRWRIERENRRHLLDKM"
     gene            395..724
                     /locus_tag="VLD60_15065"
     CDS             395..724
                     /locus_tag="VLD60_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46331.1"
                     /translation="MTQQALQKDEISRTLARIRTDALAVVCASIGGVGLCVMTMWLVI
                     KGGPHTGEHLQLLSNYFVGYSVTWPGAFVGLLYGATTGGMVGWAIARIYNAVVNARQR
                     KSGRDRA"
     gene            1257..1448
                     /locus_tag="VLD60_15070"
     CDS             1257..1448
                     /locus_tag="VLD60_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46332.1"
                     /translation="MFVFEFSILWPVSGVLILAKLLVFGVLFVLAYLALGEFSAEEMV
                     LGRSLLKMRIIPAQHPNES"
     gene            complement(1612..1980)
                     /locus_tag="VLD60_15075"
     CDS             complement(1612..1980)
                     /locus_tag="VLD60_15075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB46333.1"
                     /translation="MDEVRPYHRGLYRLPVTYPAMYCMVSTVGEGTVTNLSAIGCTIE
                     TDQPLPEDQRVALRLLLPDKLESLPIDDAQVRWVKGTRAGVEFLQVQREANLRLHGFV
                     WDKMIERIQTIQQQRSSASP"
     gene            2325..4331
                     /locus_tag="VLD60_15080"
     CDS             2325..4331
                     /locus_tag="VLD60_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HSB46334.1"
                     /translation="MTAIKLVLWAFLAILGAVALAFVTGLVYPHEKVNGLWLVVAAAC
                     IYVLAYRFYGRWLAKQVVELSNQRVTPAVRLNDGVNFHPTNKVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGSIT
                     GTATAVAVLFIVVVALAGLGFAVVNALYQNAWGTFTIAMTIPIGFIMGFYLQKFRPGA
                     VAEVSVLGVVLLIAAVLYGRVVAQSSFAPWFEYERPTLVWLLAGYGFLASVLPGWMLL
                     VPRGYLSTFMKLGVVFLLGFGVILLAPTIEMPRVTTFANGGGPIIPGTLFPFLFITIA
                     CGAVSGFHSLVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAASVLIPGDYLAI
                     NTTLGVDALTAMGFAPARITELSQLVEVDVAGRPGGAVSLAVGMASIFAALPGMAGLM
                     AYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRVYAPFRRMNWWPGVLLSSGFVV
                     GAWAYLIGTGSISTIWPMFGAANQLLGTLALCIGTTVLIKMWKSPYLWVTAVPMLFVG
                     AITLTGSYEMFGMFLAKAATLATGESFPLYLDAVLVAAVAILGLIVLSDSMKQWYGYV
                     VLKKPFTSSEVVVLAGGGSAGRMQTAIQHDDDKSFRLPHGGGCC"
     gene            4355..4852
                     /locus_tag="VLD60_15085"
     CDS             4355..4852
                     /locus_tag="VLD60_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HSB46335.1"
                     /translation="MADQFSFDVVSEVNMQELKNALEQATKEIKQRFDFKDSKTDITL
                     KEKEKELVVISDDEYKLKAVIDIIKAKCVKRGVSLKAFDFGAMEPALSGTVRQTAKIQ
                     SGLVSEKAKEITKAIKDSKLKVQAQIQGEQVRVLSKSKDELQSTMAFLKGKDFGIDLQ
                     FTNYR"
     gene            4877..5890
                     /locus_tag="VLD60_15090"
     CDS             4877..5890
                     /locus_tag="VLD60_15090"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46336.1"
                     /translation="MKILLPLLFLVVSLAGCNRSNAIVVIQLHPKNPDIMYIATNDYI
                     YKTRDGGQTWTNLSKGMSHSRVISMAIDPVYPATVYAGTKGDAVYKSHDGGQRWASMR
                     SGLDDATISSVVNQFLFDPTDNNHMFLATTMGVFETKNGGEHWTKRMEGMKEVLMVVT
                     LGMDPTRPAVLYAGTSGGVYKTVNEAGQWEKVNNGLVSPDIIKSSRALNVSSILVDPY
                     EPDTVYAATLAGFYKTTDGAKSWVRIGESLADQMIFAMVLDRTKKGVVYIAGRDGVHR
                     SDDGGATWKMMNNGLATTNVRSLAQSASDPKLFYAGTNGSGLYRSKDAGETWEPMPPV
                     AER"
     gene            complement(6056..7075)
                     /locus_tag="VLD60_15095"
     CDS             complement(6056..7075)
                     /locus_tag="VLD60_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46337.1"
                     /translation="MKTNTGRWALHLELLLIGTATFCALTALPASAASPLYYCPDRKA
                     DQRFSAANGPGCVPLVEKNERETNVNSRDKQSRDFKMQNLQSDVTTFLIKYRQFLECC
                     KTDLAELRQVEALGDEVGELLAFTQANLSNSSLASRGIMLREMMSPVARARGDLKLLR
                     ARLEKINDLSGPHDSLSSEEQAIQAQHIREIEESIEKDIRARKLPASAKTGVDIGVAP
                     AVGPGIGRTPTTGVDIGREGKTGQEIGASPRSSRDIGGSGPTGFEIGATGRAGPSIGE
                     SNLNSETSSSVNSTLQQSTVGSSIPDSTVGSSLGPSSVGSNLQDTSVGSSFGGSSVGS
                     SLQSR"
     gene            7508..8452
                     /gene="rsmH"
                     /locus_tag="VLD60_15100"
     CDS             7508..8452
                     /gene="rsmH"
                     /locus_tag="VLD60_15100"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSB46338.1"
                     /translation="MGEAGDQSFHGHRGHWPVMPTEVLSWLKPKDGEVFLDCTVGYCG
                     HAESILESSAPSGIVIGIDRDVRAIEAGRKILERFGSRAVLIKGHFVELKHHLADRGI
                     SAVQGVLFDLGVSSPQIDEPSRGFSFQAEGPLDMRMDQSAGATAAEIVNRTDEVDLAN
                     LIYELGEERYSRRIARAIVRARANRLLETTSDLVSVIEAAVPAHYRRGRIHCATRTFQ
                     ALRIAVNQELEHLEPALRDAVDVLAPGGRLCVISFHSLEDRIVKHTFRALSAQAGGLL
                     EILTKRPQFPTDEEISRNPRSRSAKLRVIQRMSKEGHA"
BASE COUNT         1697 a         2214 c         2690 g         1975 t
ORIGIN      
        1 gggcaaacgg tcgaggaact cgacgatcgc ctcatcgaag ccgcactcgc gaggctggac
       61 ccgcttgcct tgggcgtggc tgtcgggctg gttagcggtg tggggatctt gctcgcgacg
      121 gcgatcctct tgctcaaagg gggcgagcat gttggtcaga acctttcgtt gctcaggaac
      181 tatttccccg gattcgaagt gacatggacc ggtgcggcga tcggtttctt agaggttggg
      241 ctgatgggat tcgggttggg ctacctcgcg gcgtggctga gaaactggga gattgaggcc
      301 tacgccatgt tcgtacggtg gcgcatcgag agagagaatc gccgtcatct gctcgacaag
      361 atgtaaccgg cgttcacaga aagtgagtga gaacatgaca caacaagcat tgcagaagga
      421 tgaaatcagt cgcacgctcg caaggatcag aacggacgcg ctggcagtgg tctgcgcatc
      481 aatcggggga gtggggctat gcgtgatgac gatgtggctt gtgattaaag gcgggcccca
      541 cacgggggag catcttcaac tcctctccaa ctattttgtc ggatattccg tgacatggcc
      601 aggagcgttt gtaggacttc tgtacggtgc cacgaccggg gggatggtcg gatgggccat
      661 tgcacgaatt tacaacgcgg tagtcaatgc ccgtcagagg aaaagcggaa gagaccgcgc
      721 atgacccgtt cagcctgcgt gacaaggtat catgaactga actgattcct ctcagagggc
      781 tggttaggcg gtccaggagc ttgctccctt gaacaggcta agtgagaact atttcgtatg
      841 cggtgattgt aaagtggaca acacttggag caagcagaat gggtcttatg acatctccaa
      901 cgcggcttcg catgatggat gggaccgcca ggattctttt ggcggaactg ctctttcctc
      961 tgacggccct tatcacgacc ggtttcttaa ccagggagct tggttcgcag gactatggcc
     1021 tactcgcact cacgctgaca cgatcatatg gatcgagagc gcgatcaact ctttcattac
     1081 cagaggcacc ctcaaatttg tgagggagac ggtcgattgg aaaccagtcg gtgcgatggt
     1141 tgttcgtttc agtgtgaaaa tcggggtctc ggccatggtc cttctcatgg gcaatcccca
     1201 gtccgtggga tcttgcgctt ccggcgagga ccgtatggcg aagcctcata gttgcggtgt
     1261 ttgtcttcga attcagtatc ctatggccgg tgtcgggcgt gctgattctg gcgaagttgc
     1321 tggtgttcgg agtcctcttt gtgctggcct acctggcact tggtgaattc agcgcagaag
     1381 aaatggtgct cggtcggagt cttttgaaga tgcgcatcat ccccgcacag catcccaacg
     1441 aaagctgacc gcgaaccagg acggcggcaa tctgcttctc cttcaagcct tcggctacgc
     1501 cgatgtgacc ggcatcacct ttgcgcttct acggcggttc cgcgaactgg cgtggatcgg
     1561 aatcgggttg ctgtgtccgg cggcaatcgg aaaatacgga gcgcgacagc gttagggcga
     1621 agcacttgag cgctgctgct ggatggtttg gatccgttcg atcatcttgt cccacacgaa
     1681 accgtgcaag cgaagattgg cttcgcgttg gacttgcagg aattccacgc cggctctggt
     1741 gcccttgacc caccgcactt gagcatcgtc aatcggcagt gactcgagtt tatcgggaag
     1801 cagcaggcgc agcgccacac gttggtcttc gggcaacggc tgatcggttt cgatcgtgca
     1861 tccgatggca gaaagattcg tgaccgttcc ttcacccacc gtcgagacca tgcaatacat
     1921 cgccggatag gttacaggca gccggtagag gccgcggtga tagggtctga cttcatccat
     1981 agtgaagtta gtctagcctg agagggccga gaaatgtatc cctcctaagg ggggcgtgtg
     2041 ccagtttgga tgtgcgccgc caccacactg tctgaatcac ccaactacgc cttgcatgtg
     2101 cgaagcgccc gcgctgcctc tgctttcgcc ggcgcctgcc ggctcgccgt gacgctcagc
     2161 caggcttggc ccagagaggt ggggggcggc ctagttgtcc ttgtgcttgc ggaacgtggc
     2221 acgcgggatc gatcgtatgt gtgtatcaag cgaggtgcgc gctccgtgag caaggaatca
     2281 acgcgggtgc cgttacagtt cccctttatg ataggatgcc gcgcatgacg gcgataaagc
     2341 ttgtcttgtg ggccttcctc gctattctcg gtgccgtggc gctcgctttc gtgaccggcc
     2401 ttgtttatcc ccacgaaaaa gtgaatgggc tctggctggt tgtcgcggcg gcttgcattt
     2461 atgtgctggc ctaccgtttc tatggccgct ggctggcgaa gcaggtggtg gagctgagca
     2521 atcagcgtgt gacgcctgcg gtgaggttga acgacggcgt gaattttcat cccacgaata
     2581 aagtcgtcct cttcggccat cacttcgcgg ccattgctgg cgctggtccg ttgctcggtc
     2641 ctgtgctggc cgcgcagttt ggatttcttc ccggctttct ctggctggtg atcggtgcgg
     2701 tgctggccgg agcggtgcag gatttcatca tcctcgtggc ctcaatgcgg cgcaatggac
     2761 gctcgctgcc cgagattgcg cacgatgaac tgggttccat caccggtaca gcgacggcgg
     2821 tggcggtgct ctttattgtg gtagtagcgc tggcggggct gggcttcgcg gtggtaaatg
     2881 cgctctatca gaatgcctgg ggaacattta cgatcgcgat gacgatccca atcggcttca
     2941 tcatgggctt ctacctccag aagtttcgcc ccggtgcggt ggccgaagtc tcggtgctcg
     3001 gagttgtcct gttaattgca gcggtgttgt acgggcgtgt cgtggcgcag tcgtcgtttg
     3061 cgccgtggtt cgaatacgaa aggccgacac tggtctggct gctggccggc tatggcttcc
     3121 tggcgtcggt gctgccgggc tggatgttgc tcgtgccgcg cggctatctc tccaccttta
     3181 tgaagcttgg cgtcgtattt ctgctcggct tcggtgtgat tctcttggcg cccacgatcg
     3241 agatgccgag ggtgacaacg ttcgccaacg gcggcgggcc gatcataccc ggcacgctct
     3301 ttccctttct tttcattacg attgcctgcg gggctgtctc gggcttccat tcgctcgtgt
     3361 cgtctgggac gacgccgaag atgatcgagc aggaatcgca ggcggtagtg ggttatgcgg
     3421 cgatgctgct cgaaagtttc gtcggcgtga tggcattgat tgcggcctcg gtgctgatcc
     3481 ccggcgatta tctggcgatt aatacgacgt tgggcgttga tgccttgaca gcgatgggtt
     3541 ttgcaccggc gcggatcaca gaactgtcgc agcttgtcga agtggacgta gcggggcgcc
     3601 cgggcggggc tgtgtctctg gcagtcggca tggcctcgat ctttgcggcg ctgcccggga
     3661 tggccgggtt gatggcctat tggtatcaat tcgcgctggt gttcgaggcg ctcttcatcc
     3721 tgacgacgat cgatacggga acacgcgtgg cgcgctatct gattcaggaa atggcggggc
     3781 gtgtttatgc accgttccgc cggatgaact ggtggcccgg cgtgttactg agcagcggct
     3841 tcgtggtcgg ggcctgggcc tacctgatcg ggacggggag catttcgacg atctggccga
     3901 tgttcggcgc ggcgaatcaa ctgctgggga cgctggcgct ctgcatcggg acaacggtct
     3961 tgatcaagat gtggaagtcg ccctatctct gggtcacggc ggtgccgatg ctcttcgtcg
     4021 gtgcgattac gctgaccggc tcctatgaaa tgtttggcat gttcctggca aaggcggcga
     4081 cgctggcgac gggcgagtca ttcccgctct atctggacgc ggtgctggtg gcggcggtgg
     4141 cgatcctggg attaatcgtc ttgagcgaca gcatgaagca atggtacgga tatgtggttt
     4201 tgaaaaagcc cttcacgagt agtgaagtgg tcgtcctggc gggcggggga tcggcggggc
     4261 ggatgcagac ggcgattcag catgatgatg acaagagctt ccgcttacct cacgggggtg
     4321 gatgctgttg aaatgggatc attaggaggc gaccgtggca gatcaatttt cttttgatgt
     4381 cgtatcggaa gtgaacatgc aggagttgaa gaacgcgctc gagcaggcga cgaaagagat
     4441 caagcagcgc tttgatttca aggactcgaa gacggatatc acgctgaagg agaaagagaa
     4501 ggagctggtt gtcatctcgg acgacgagta caagttgaag gcggtgatcg acatcatcaa
     4561 ggccaagtgc gtgaagcgcg gggtatcgtt gaaagcgttc gactttggtg caatggagcc
     4621 ggcgttgagt ggaacggtgc ggcagaccgc gaagatccag agtggacttg tctctgagaa
     4681 agcgaaagag attacgaagg cgattaagga ttcgaagctc aaggtacagg cgcagattca
     4741 gggcgagcag gtacgggtgt tgagtaagag caaagacgaa ctacaatcga cgatggcctt
     4801 cttgaagggg aaggacttcg gaatcgatct acagttcacg aactatcgat aagggggtgg
     4861 cgctcacgac cgtctcatga agattcttct tcctctgctc tttctcgttg tttctctcgc
     4921 cggctgcaat cgcagcaatg ccatcgttgt gattcaactc cacccgaaaa atcccgacat
     4981 catgtacatc gcgacaaacg actacattta taagacgcgc gatggcgggc agacctggac
     5041 gaatctctca aagggcatga gccattcgag agtcatttcc atggcaattg atccggtcta
     5101 tccggcgaca gtctatgctg gcacgaaagg cgatgccgtc tacaagagcc acgacggcgg
     5161 acagcgctgg gcgtcgatgc gttcagggtt agacgatgcg acgatttcat cggtcgtcaa
     5221 tcagttctta ttcgatccga cggacaacaa tcatatgttt ctcgcgacga cgatgggagt
     5281 gttcgagacg aagaacggcg gcgagcattg gacgaagagg atggagggca tgaaggaagt
     5341 gctgatggtg gtgacgctcg gcatggatcc gaccaggccc gcggtcctct acgcgggcac
     5401 gagcggcggc gtctataaaa cggtgaatga agccggccag tgggagaagg tcaataacgg
     5461 cttggtctcg cccgatatca tcaagagttc acgcgcgctg aatgtctcgt cgatactcgt
     5521 cgacccatac gagccggata cggtctacgc cgcaacattg gcggggttct acaaaacaac
     5581 ggatggcgcg aagtcctggg tgcgcatcgg cgagtcgctg gccgatcaga tgatcttcgc
     5641 catggtgctc gatcgcacaa agaaaggcgt ggtctatatt gccggacggg acggtgtgca
     5701 tcgcagcgat gatggcggtg cgacatggaa gatgatgaat aacgggcttg cgacgacgaa
     5761 tgtgcgttcc cttgcacaga gtgcgagcga tccgaagctc ttctatgcag gcaccaacgg
     5821 cagcggcttg tatcgaagca aagatgccgg ggaaacctgg gagccaatgc cgcctgtagc
     5881 ggaacgctga aaaaatctgt cagcatcgtt ttcgcatcat tcagtggctc aacattctga
     5941 aaaacgtata cctcgcctct tcactcgctg cggccttgtt gtggaaaggc gcttcttggc
     6001 gcgccagggt gggcgggcga gaagcccagc ctttttgagc gttcccgact tcaggttatc
     6061 gtgattgtaa gctggagccg acggacgagc ccccgaaaga cgaacccacc gacgtgtctt
     6121 gcaagtttga gccgacgctc gagggcccaa gactcgaccc cacggtcgaa tccggaatgc
     6181 ttgagccgac ggtcgactgc tggagtgtgg aatttacgct cgaggaagtt tcgctgttta
     6241 agttcgattc accgatcgag gggccggctc tgccggtcgc accgatctcg aatcccgtcg
     6301 gcccgctccc accgatatcc cgactactgc gaggggaggc gccgatttcc tgaccggttt
     6361 tgccttcgcg cccgatatcg acacctgtcg ttggtgttcg tccgatgccg ggacccacgg
     6421 cgggggccac gccgatgtcc acaccggttt tggcgcttgc ggggagcttc cgcgcgcgga
     6481 tatctttttc gatcgattct tcgatctcgc gtatgtgctg cgcttggatc gcctgttcct
     6541 cagaactcag agagtcgtgc gggccggaca agtcgttaat tttctccagt cgcgcgcgca
     6601 agagtttgag atctcccctc gccctcgcca ccggcgacat catctcgcgc agcataatcc
     6661 ctctcgaggc caacgaacta ttcgagagat tggcttgcgt gaaggccaga agttctccca
     6721 cttcgtcgcc aagcgcttcg acctgccgaa gttccgcaag gtctgtctta cagcactcga
     6781 gaaactgtcg atacttgatg agaaaggttg tgacatcact ctggagattt tgcattttga
     6841 agtcgcgcga ctgtttgtct ctggagttca cgttagtttc tctctcattc ttctccacga
     6901 gaggcacgca gccgggaccg ttggcagctg agaatcgttg atcggccttg cggtcgggac
     6961 agtagtaaag aggcgatgcc gcagatgcgg gcaatgcagt gagagcgcag aatgttgccg
     7021 ttcctattaa cagaagctcc aggtggagcg cccatcgtcc cgtgtttgtc ttcatgcgaa
     7081 cattctcatc ccttgccgga accttgtcaa tgaaacgaaa gaacttcgcg ttcagaaacg
     7141 gtccaggcat cgttcgggcg gctcctctat tttccatttc tggaagaact gatttcttga
     7201 agaagctggg gatgacacta gaccttgctt ggatctcccg ccaggaacac ctgcgctatg
     7261 cgaatcctgc tccacggaat gccacagaaa agggcctctt tccgccaggc tccctctagc
     7321 gcctcgcaaa aggccactct attttcataa cgggaggctt cgattgaccc acttctgagt
     7381 ccgattctcc aattttttct gggtcgaaat acggcatttt caagacccgg ggaaaattca
     7441 taagcttatg aatttcttgt tgacaaggtc ttaacgagga gtatactgcg ccgctaagtg
     7501 ggagaaagtg ggtgaagctg gagatcaaag ttttcatggt catcgagggc attggcctgt
     7561 catgcccacg gaggtgcttt cttggcttaa gccaaaagat ggagaagtct tccttgattg
     7621 cactgtgggg tattgtgggc atgcggagag tattcttgag tcgagcgctc cgagcggcat
     7681 cgttattggc atcgaccggg acgttcgagc catcgaagca ggcagaaaaa tactggagcg
     7741 gttcgggagt cgggccgtcc tcatcaaggg gcattttgtg gaattgaaac atcacctggc
     7801 tgaccggggt atttcggcgg tgcagggagt gttgttcgat ctgggagtgt cgtcgccgca
     7861 gatcgatgaa ccgtcccgcg gttttagttt ccaagcggaa ggtccgttgg acatgcgaat
     7921 ggatcagtca gccggtgcga cggcggcgga gattgtcaac cggaccgacg aagttgatct
     7981 tgcgaacttg atctatgagc tcggcgaaga gcgatattcg cggcgcatcg cgcgcgcgat
     8041 cgttcgggct cgcgccaacc ggctgttgga aacgacgagc gatttggtgt cggtgatcga
     8101 agcggccgtg cccgcgcact atcgccgcgg gcgcattcat tgcgcgacac gcacatttca
     8161 ggcgcttcgc atcgctgtga accaggagtt ggagcatctt gagccggcgt tgcgtgatgc
     8221 cgtcgatgtg ttggcgcccg gcggacggct ctgtgtgatt tcgttccatt cactcgaaga
     8281 ccgcatcgtg aagcacacgt ttcgggcgct ttccgctcag gccggcggcc tgttggaaat
     8341 tctgacgaag cggcctcagt ttcccactga cgaagagatc agccgcaatc ctcgatcgcg
     8401 cagcgcgaag ctccgcgtca ttcagcggat gtcgaaggag ggacacgcat gaaagccatc
     8461 atgatcgtgg ccgggatgtg tttggttttc atcttcgtgt gggaacgggt cgacgtcgtg
     8521 cgattgggtt accacaccga acgattgaag gcccaaaagc tcttgctgga gcggga
//
LOCUS       DATIFR010000261         5449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1719130, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000261 DATIFR010000000
VERSION     DATIFR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719130"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(469..1149)
                     /locus_tag="VLD60_15105"
     CDS             complement(469..1149)
                     /locus_tag="VLD60_15105"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSB46339.1"
                     /translation="MRRIVRIGHRGAAGHAPENTVAAIQKGIALGVDFVEIDVRRTAD
                     GILVALHDETLDRTTNGKGRVDRLSLAEIQPLNAGNGEHIPTVVEVLKTAAGKAGLML
                     ELKVSGIAHETVEAVREAGFKDPVIYASFLHDELTDVRSVDPEASLMVLFGRLPLAPV
                     ERAMKFKPSHVGLRHDTATRHLVDAFHDKGFLIFVYTANSLGDIQRALSVGVDGVISN
                     FPERVRRE"
     gene            complement(1199..2413)
                     /locus_tag="VLD60_15110"
     CDS             complement(1199..2413)
                     /locus_tag="VLD60_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB46340.1"
                     /translation="MPKGSNKYGKILFVFCGGGCKAIIQAIAAAEVVNAGLVPSHIVS
                     SSAGSCNALGFVENPGVIGAEKTIRIWEEYINSPAAIYEVHPFLREKLGRFLGVVPRV
                     TQGWGPSGSILHDLRRAINFLPLVLSFCMRMPFRVAGRAVSFVFRLMDTFESQHKSFR
                     RLVQAPEIQEAFDEMDKYFEFKRMKAFLDPFPLLARLGEQINLQKVLTSPIVWHIITE
                     RYEDGATVVFSNKDPDLTMDGSEDTRSQKAKYDAFFKRIRASMALYPLFEMVEMNGHR
                     YLDADLANPLPVEEAFGCGCDTMFIFLNVPTKSVRVEPHPLRDILELNDLLRLNERYI
                     HHRVKEAQVKAKELGVNLFVIRPDEIPPGLGLLEIDGKVIELVKNHERKRMKEYLANL
                     DAHNLRFAPPIY"
     gene            2524..3699
                     /locus_tag="VLD60_15115"
     CDS             2524..3699
                     /locus_tag="VLD60_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB46341.1"
                     /translation="METYDVCIVGAGVVGCAIARELGQRYRDRPLRIVVLEQREGPGE
                     ETSARNSGVLHSGIHEHPRSLKARLAREGSALAVSYALQHGIPLLRKGMVIAIAWEDV
                     RRGLWRELSMLRRLWINAWKTRIRVSFVTPRGLRAWEPNLRTCCGIVIPSVCVIDPVA
                     FAQSLRTDSELSGATFAFNNRVIGIDEEAARYIVTTDRKRIRATCLINAAGLNADDIA
                     ALALHNKKYTIHPLRGEYYELIAPEKKSLIGRLVYPALPSGATGKGIHFGPRPNGQLF
                     VGPNEVPVQDKTDYTSQKTPPGMFIEALQKFLPALDERDLRWAYSGIRPCVMSGNGRK
                     SDFIVSVDREDPPLINLIGIESPGLSAALALARHVGDLPCIQSHLRPSRSRFPSNGA"
     gene            complement(3696..5360)
                     /locus_tag="VLD60_15120"
     CDS             complement(3696..5360)
                     /locus_tag="VLD60_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSB46342.1"
                     /translation="MPDQPWFLHYPEGVPHELSITPETLLAMFSRSTQRFSRRPALYF
                     FGKTLSYQECHALIERFACGLAALGIRKGDRVALCLPNSPQAVIAYFGILRAGGIVVA
                     CNPTYTEHELSHQLRDAGARAIVVLDLMYQKVEHLNLELIVVTRITDFMTTIAKYLYQ
                     HRSGHPPLEIPARDTTVFFHDVLHRVTPVEPEKLPLVTPDDIAVLQYTGGVTGVSKGV
                     ELRHRNLTANVRQLISWFSPAERVESFLAALPLFHVFGMTVTQNICLAVGGCMVLVPD
                     PRNMPVLLTLLRKKQPTVITLVPAFVRKLLEHCEAKDFTATRFFICGGAPLPYELLKK
                     FKEKTGHLIVEGYGLSEASPVTHCNIPRGLSVKRSIGLPIANTEVKIVDEAGHEVPDG
                     EIGELWVRGPQVMERYWNNPVETANVMKPGGWLATGDIARTDQDGFFYIVDRKKDMIL
                     STSGFNVYPAEIEQVLLLHPLVREAAAIGVKLGNGSESIKAFVVPKTESVSADDLLAH
                     CRRYLAAYKVPKKIEFRTELPQSILGKMLHRVLRVEEGARLQLQRS"
BASE COUNT         1184 a         1583 c         1497 g         1185 t
ORIGIN      
        1 gttcggtcac ttgatcgcgg gctcagcgca ggacgtgttc tatgggaacc tgctcggtgc
       61 cgcagccatg gtgaccggct ccatcgtcgc cctaagattt ggggtcagtg ccgaacggca
      121 gcccttggag cacatcgccc cgcccctttc ttgccgagac actcatacca tgtagtagtc
      181 aacagcccgg acgactatca tccacagatg aaacacggcg cattcgtgcc caactgatca
      241 ggacttgtga tttcccggca ccgaagccgg catcgccggc ggcgcaagca tcttccgcta
      301 tgcccttgcc tatggcctta cgaacgggat gatccctggt tagctgaatt ctcataccac
      361 ctatctatat tgttttcagc ccatgcttgc ctcttctcgg caaacaattg agtcaccaac
      421 tcaccactct ccccctagaa aagctcaaac gccgttatag ctgtcgaatt actctcttct
      481 gacacgctca ggaaagttcg atatcacacc atcgacgcca accgagagcg cgcgctggat
      541 gtcacccaag ctatttgccg tatagacaaa tatgaggaat cccttgtcgt gaaaggcatc
      601 gacaaggtgg cgggtagcgg tgtcgtgccg gagcccgacg tgtgagggct taaatttcat
      661 cgccctctcg accggggcaa ggggcagcct accgaagagc accatgagag aagcctctgg
      721 gtcgaccgat cgaacgtcag tcagctcgtc gtgaaggaag gaggcataga tgacagggtc
      781 tttaaatcca gcctcccgta ctgcttctac tgtttcatga gctattccgc taaccttcag
      841 ttccaacatc agccctgctt ttcctgcagc agtcttgagc acttccacca cggttggaat
      901 gtgttcgccg tttcctgcat tcaatggctg tatctccgcc agcgataggc gatcgacccg
      961 tcctttccca ttcgtcgttc gatccagagt ctcatcatga agggccacga ggatgccgtc
     1021 cgccgtgcga cgcacgtcaa tctccacgaa gtcgactccg agagcgatcc ctttctgaat
     1081 cgcggccacg gtgttttccg gggcatgccc cgcagctcca cgatggccaa ttctcacgat
     1141 gcgacgcatg gggtttctcg ggaccaacgc cgacgaggca cacgagatgg gttagtcgtc
     1201 aatagatggg tggcgcaaat cggagattgt gcgcatccaa attggcgaga tattccttca
     1261 tacgttttcg ctcgtggttc ttcacgagtt cgatcacttt gccatcaatc tccagcaatc
     1321 cgaggccggg agggatctcg tctgggcgaa tgacaaacag attgacgccc agctccttgg
     1381 ctttcacctg tgcttccttg acgcggtggt ggatgtaccg ctcgttcaac cgtaagaggt
     1441 cgttcaactc cagaatatct cgcaggggat gcggctcgac acgaacgctc ttcgtcggaa
     1501 cgttgaggaa gatgaacatc gtgtcgcatc cacagccgaa ggcttcctcg actggaaggg
     1561 ggttggcgag atcggcatca aggtaccgat gcccgttcat ttcaaccatc tcaaacaaag
     1621 ggtacagggc catggacgcg cgaatgcgtt taaagaaggc atcgtatttg gctttctggc
     1681 ttcgcgtatc ctcactcccg tccatcgtaa gatcgggatc tttattggaa aagaccacgg
     1741 ttgccccgtc ttcgtaccgc tcggtgatga tgtgccagac gatgggactt gtgagcacct
     1801 tctgcagatt gatctgctcg ccgagtctgg ccaagagcgg aaacggatca aggaacgcct
     1861 tcatcctctt gaattcaaaa tacttatcca tctcatcgaa ggcctcttgg atttcgggtg
     1921 cctgcaccag tcgtcggaag gacttgtgtt gggactcaaa cgtgtccatg aggcgaaaca
     1981 cgaagctcac ggcgcggccc gctacccgaa acggcatgcg catacaaaag gacaggacca
     2041 ggggtagaaa gttgattgcc ctcctgagat catggagaat cgatccgctc ggcccccagc
     2101 cttgcgtcac ccgcggaaca acccctaaga aacgtcccaa tttctctcga agaaaggggt
     2161 ggacttcata gatcgctgcc ggggagttga tatactcttc ccagatgcga atggttttct
     2221 cggccccgat gacgccaggg ttctcgacaa acccgagagc gttacagctt cctgcggagg
     2281 aactcacgat gtggctcgga accaaaccgg cattgaccac ttctgcggca gcaatggctt
     2341 ggatgatggc tttacatccg cccccgcaaa acacaaacaa gatcttcccg tacttattcg
     2401 aacctttcgg catgggaatc ctccggcctg atgattcgag gacagccgct gccttgagaa
     2461 cctgtcccga cgaccccacc acggtttgcc cggcctattc ttggatccaa gggacccgac
     2521 cagatggaaa cgtatgatgt ctgtattgtc ggcgctgggg ttgtcgggtg tgccatcgcc
     2581 agggagttag ggcaacggta ccgggaccgt ccgctccgca tcgttgtgct cgaacaacgt
     2641 gaggggccag gcgaggagac cagcgcgcgc aacagcggag tgctccatag tggtatccat
     2701 gaacaccctc gttcattgaa ggcacggctg gcccgagagg gaagcgcctt ggcggtctcg
     2761 tacgccttgc agcacggcat tcctttgctg aggaaaggaa tggttatcgc cattgcctgg
     2821 gaggatgtcc gtcgaggcct ctggcgcgaa ctgtccatgc ttcggcggct gtggatcaac
     2881 gcctggaaga ctcgcatccg ggtatccttt gtgacgccgc gcggattgcg tgcgtgggaa
     2941 ccgaacctcc gtacctgttg cggaattgtc attccgagcg tttgcgtcat agaccctgta
     3001 gccttcgcgc aatcactcag gaccgattcg gaactgtctg gtgcaacgtt tgccttcaat
     3061 aaccgggtca tcggtattga tgaagaggcc gctagataca tcgttaccac cgaccgtaag
     3121 aggatccgtg ccacctgcct gattaacgct gcaggactca atgccgacga tatcgctgcc
     3181 ctcgctctac acaacaaaaa gtataccatc cacccgctgc ggggtgagta ctacgagctc
     3241 atcgccccgg agaaaaaaag ccttatcgga cgcctggtgt atcccgcttt gccttccggg
     3301 gcaacaggca agggcattca tttcggtcct cgaccgaacg gacagttgtt cgtgggaccg
     3361 aatgaggtgc cggttcaaga caagacggac tatacgtcgc aaaaaacgcc accgggcatg
     3421 ttcatcgagg ctctgcagaa atttcttccg gcgctggacg aacgcgatct tcgctgggcc
     3481 tattccggaa tccggccctg cgtcatgagt ggcaatgggc gcaagagcga cttcatcgtc
     3541 tctgtagata gggaagaccc gccgctcatt aatctcatcg gcattgaatc gccggggctt
     3601 tccgctgcct tggcactcgc tcgacatgtt ggcgacctgc cttgcatcca gagccatctt
     3661 cgtccatccc gttctcgctt tccctccaac ggcgcctagg aacgttgaag ttggagtcgc
     3721 gccccttcct cgacacgcaa cacccgatgt agcatcttcc caagaatcga ttgtggcagt
     3781 tcggtgcgga attcgatctt cttaggcacc ttgtaggccg ccaaatagcg ccgacagtgt
     3841 gcaagaaggt cgtctgcaga gacagactca gtcttgggta cgacaaaggc tttgatggat
     3901 tcgctcccgt ttccgagctt cacgccgatg gctgccgcct cgcgcacaag tggatgaagc
     3961 aggagcacct gctcaatctc ggcggggtac acattgaagc ccgaggtcga cagaatcatg
     4021 tctttcttcc ggtccacgat atagaagaag ccgtcctgat ccgtgcgcgc gatgtcgccg
     4081 gtcgcgagcc agccgcccgg cttcatcacg tttgccgttt ccacggggtt gttccagtac
     4141 cgttccatga cttgcggacc tcgaacccag agttccccta tctccccatc cggtacttcg
     4201 tggccggcct cgtccacgat tttgacttca gtgttcgcaa tgggtaatcc aatggatcgc
     4261 ttgaccgaca gccctcgggg aatgttgcag tgcgtgaccg gagaggcttc ggagagacca
     4321 tagccctcga cgatcagatg cccggtcttt tctttgaatt tcttcaggag ttcataagga
     4381 agcggggccc ctccgcaaat gaaaaacctc gttgccgtaa agtccttcgc ctcacagtgc
     4441 tcaaggagct ttcgcacgaa ggcaggaacg agtgtaatca cagttggttg tttcttccga
     4501 agaagcgtaa gcaaaacggg catgttgcga ggatccggga ccagcaccat acatccgccc
     4561 acggccagac agatattttg cgtcaccgtc atcccaaaca cgtgaaagag cgggagcgcc
     4621 gcgagaaaac tctccacccg ctcggcggga gaaaaccagc tgatcagttg ccggacgttg
     4681 gctgtcagat tgcggtgacg cagctccact cctttggata ccccggtaac accgcctgtg
     4741 tattggagga cggcgatatc gtccggagtc acgagcggaa gcttctccgg ctcgacggga
     4801 gtcactcgat gaaggacatc gtggaaaaac accgttgtgt ccctggctgg aatctcaaga
     4861 ggcggatggc cgcttcgatg ttgatagaga tactttgcga tcgtcgtcat aaaatcggtg
     4921 atccgcgtga caacgatcag ctccagattg agatgctcta ctttctggta catgagatcg
     4981 agcaccacga tcgcccgcgc ccccgcgtcc cgaagctgat gggacagttc gtgttccgtg
     5041 taggtcgggt tgcaggccac gacgatcccc cctgccctca agataccgaa gtaggcgatg
     5101 accgcctggg gagaattcgg aagacagagg gccacgcgat cgcccttgcg gatccccaac
     5161 gccgcgaggc cgcaggcgaa tcgttcgatc agggcatggc attcctggta cgacaaggtc
     5221 ttgccaaaga aatagagagc cggccggcgt gaaaaccgct gcgtgctacg ggaaaacatg
     5281 gccaagagcg tctccggagt aatggagagc tcatggggca ctccctcagg ataatggaga
     5341 aaccacggtt gatcaggcat gaagcgctcc gttttggttg gcccaagaaa gtcttgccac
     5401 taccactctc cgttcgagag acccgggcag ggctcgaagc gtttgccat
//
LOCUS       DATIFR010000262        17550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_2486867, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000262 DATIFR010000000
VERSION     DATIFR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486867"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VLD60_15125"
     CDS             complement(<1..588)
                     /locus_tag="VLD60_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46343.1"
                     /translation="MSSSWTRLTNAFDWKKLFSAEIRHEDTAGREAAEAYAEWRRNFI
                     RERLLVLNYLGFIANPAFIALDYLQHREYLIPLFMIRVVLQLGLLCGFLLLKYGPKSI
                     PPNWFVILWVVVANLCVTQMTLILGGFSSQYYNGLNLVYLTAAVIVPVSWPSHLAGQL
                     VTLSSYYVSNFLGVQPNLNAAIENSFFLIWTCVALL"
     gene            complement(657..2030)
                     /locus_tag="VLD60_15130"
     CDS             complement(657..2030)
                     /locus_tag="VLD60_15130"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSB46344.1"
                     /translation="MVSTRKAVARWVLLLAVLGAHILTTRTAHALNGPQFIGFSAEST
                     ALAASGHVAVADTSAINTNPAAMSLIQGTRFDVTAGFIQAFLHHSDVFGNTNVGGQNN
                     AYALGNLGVATRLASVPGLTIGAGIFTQGGFGSDYRNLRTAFGTQDAASSFFRYLKFA
                     IGLSYEATEKLSFGIAPSVGYSDVSLRLFPGTSVLPSPGLPAGFAGFNIRDSCARNGG
                     LGPLGGDCPSDVVFSVKVGAMYRALPWLTVGATYTSPVRFNYTGGQATLNFSAFGLGT
                     VNYDARVAGIKWPQQVDVSMAAKPMEQFTVALTTSWINWATINTVEITATNPNNPQAP
                     SQVNLNIPFNWKDQVVVALGFSYSAMQELSWKDKDRLVLRAGYNYSNNPIPKETLSPL
                     WPLINEHHFSVGLGFRFTERWSYDFSGLYAPKNSVTYTNPSLPFGPNATASISGYDIY
                     NTISYRF"
     gene            complement(2758..3654)
                     /locus_tag="VLD60_15135"
     CDS             complement(2758..3654)
                     /locus_tag="VLD60_15135"
                     /inference="COORDINATES: protein motif:HMM:NF039293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46345.1"
                     /translation="MADQDLHLTPGTLQASLLERTAHARRCGAIQFLPTTVEVIEQDG
                     IPFQVRVLESLTMKEIAKAARSNVNPFLPYDPDLFVAPISPTHIALLNKFNVVERHLL
                     IVTRAFEDQRCQLTQKDCEALLIGLTEIDGLGFYNAGHEAGASQSHKHLQLVPFDATI
                     GPLPIEPFLRFAQTDGMIGTVPRLPFRHAYAPMDRDWTDPEKDSGASLHACYRKLLHA
                     TGLSVDATTGSHALTAPYNLLVTRRWMLVVPRSQECFEGISVNALGFAGAFLVKDRRQ
                     LATLRRFGPMTALQHVTGPSSR"
     gene            complement(3863..3938)
                     /locus_tag="VLD60_15140"
     tRNA            complement(3863..3938)
                     /locus_tag="VLD60_15140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3903..3905),aa:Thr,seq:cgt)
     gene            complement(4036..5958)
                     /locus_tag="VLD60_15145"
     CDS             complement(4036..5958)
                     /locus_tag="VLD60_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46346.1"
                     /translation="MPHGWLPIISRRPAGSLCVWIVSLALTGCALFSSRDGAGIGYYL
                     PLTVQLRPASSIAAAQVQYQDACGYPKSLPIGAMLEEAVKRKSGRVFEKVVIDAPGTI
                     AVDGYEDVSLGMTRLDLVISQKANRSYPATLAIGLDFAYTAADGTVLFSKKLQSVGRG
                     EVDVTESSCEVKGLDKLAQEAISNVTDGMAKQLGTSSKILMAAEAPKTGAPRTGGVVP
                     PPPIAAGGANEAVTAQPKDTAPVPPPAADEASTLVFRAIIRDENRNQLLHSGETLGVE
                     IEVKNEGPGSASGVEVLVSGTPALVEHIPSVLPVGDLPAGDVKRVSLDGKVGTVTEAG
                     QAELVLALRSQSGGAHIPAAKKFLVAIKPADAPEAAMVPVDVDEVPKNSGKLKQPKAV
                     GIAIGIGQYRETGLPRVKFAAQDAKVMAKYWSAIGGIPPDRIRRLIDSHALKTDVAEV
                     FEEWLPKHVDPTTVVYVYISGRGIVEPTTGAVSVIPFDGTASSGARVYSLRRLQESLV
                     KLPIHRAIIMLDLSLEYGQLPEGATHAAPLWEMEGQDKDKIMWMVGNRAVQEAHAYDL
                     GQHSLYTYHLLRGLGGAADIDKDGTIFAGELCAYSRGRAAKMAREQFGDDQEPLCLPG
                     PGQGAMVRLQPLAKLK"
     gene            5950..6957
                     /gene="ubiA"
                     /locus_tag="VLD60_15150"
     CDS             5950..6957
                     /gene="ubiA"
                     /locus_tag="VLD60_15150"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HSB46347.1"
                     /translation="MRHSHNDLYTEIHPAITTQGQFDAIDRSRSLGYVGSMIAPSAPP
                     HDVPSQSNFSWSAVTRLIRLRNQTGTWLLLLPTLWSLILAARGIPPWLLIVIFSLGSF
                     LMRSAGVVLNDLADRPFDRHVTRTQQRPLASGELRPWHALVVVGILLAGAGLLVLLLN
                     SFTIMLSPIALLLAGLYPFAKRVVQIPQAMLGIAFGWGTIMAWAASDGTIEAPAWLVF
                     AATVCWAVGYDTIYALQDREDDERIGVKSSALFFGSSAWLAVGVSFIAMVALLGLAGW
                     LTQIGWFFYAVLAGVSLYCATQALKLRNPVAPLPAFDMFRRHVWVGSAILVGLIGGFL
                     V"
     gene            complement(7141..8157)
                     /locus_tag="VLD60_15155"
     CDS             complement(7141..8157)
                     /locus_tag="VLD60_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB46348.1"
                     /translation="MKAARLQVIGAVAGLMGVWAMVAGGCASEQQRKGQELYAHYCMH
                     CHGENGRQNEGFNWSSMPDPKPKDLSNKSEMGTFKDEDIFNTISRDMKDTSPGGDKIG
                     DDDFAVPTMPTFKYTLSEDEIWAIVGYVRTLHGMKLEFKVEERKKELAEALKTAQEKF
                     DQAKLTYEAAEKKASEEAEKKSEALKKDVEVDESSYAKEQTAMTQAKKELDTAQAALN
                     NFSTRPGKGVNVARPDLTMKPEQAAQLAEVGKRLYSNKYGCNGCHSLGEEGGKVGPAL
                     DRAGFRLNSTWVYRWVKNPQAMKPETRMPALGLSDADAKAVSMYLSTLRAPKAEPPAD
                     KQGS"
     gene            complement(8154..8987)
                     /locus_tag="VLD60_15160"
     CDS             complement(8154..8987)
                     /locus_tag="VLD60_15160"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSB46349.1"
                     /translation="MTRGMRQGIQWGSTVAGFGIALFVAGTTVSLAQESVAVRALMVK
                     GALPVDDPNAAAWNSAPQAEFPMSPQVHWQNRIQEVTVKDLKVRALHDGTQVAVLLEY
                     ADPTENPDDAAALEFMVGDKKAHFAHGQPMAQVEGGPVNIWYWKNKDNKGVDMSAKGF
                     GTLKAQDHQDVKAKGVYANGSWKVVFSRNLSTDHPDEDTQVKPGEFINVAFAVWDGRK
                     DGAGDLVEKGSQKAVSSWWYFRAEAPPDYSGYLYAALAMGLALAFQFVLIRKLKKGQS
                     A"
     gene            complement(9033..9749)
                     /locus_tag="VLD60_15165"
     CDS             complement(9033..9749)
                     /locus_tag="VLD60_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB46350.1"
                     /translation="MGGLTKPAILIAVVYLILKFVVPNIPGSAPLPSSLIFLYIMLTT
                     SGIVIFATMSGESKDAFWGPIQRFLTGENIGALQAARYAVLIVFPLLVGWQTYSSTAV
                     SDAPPAENRTIHPAPPGEYTGLSNPVAKTPDNIMQGKGFYAAFCSPCHGGNFDGKGPA
                     ARGFNPPPANFSDPTTIAMLQESYLFWRIKKGGVGLPIEGMPWKSAMPRWEVELPDEW
                     IWKIIMGEYDGAHQSPRTWE"
     gene            complement(9760..10383)
                     /locus_tag="VLD60_15170"
     CDS             complement(9760..10383)
                     /locus_tag="VLD60_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB46351.1"
                     /translation="MSEVVKLQLIGLSVVGFGIVTLLFVRAQFARVIGFVAIVLGLFS
                     LVALAVPQMASLPPAEEKFDIATVKTPTDMAAIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTQPQAYVYLDYRHEGLPKEYPARMPYINKNPIGLTKNEI
                     LSVIAFLQQMSGEPISVNPSELEVPGQAPAAPVKAAESVPLAVARAH"
     gene            complement(10478..12325)
                     /locus_tag="VLD60_15175"
     CDS             complement(10478..12325)
                     /locus_tag="VLD60_15175"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSB46352.1"
                     /translation="MRHQGGLIQRILQTLSGHGGWLPGLLLAAGWLALPSIGIAAEGA
                     PAGPTEYRDIPGIGSRNLVWIIAQLHLLLAGFVLGVPIFAWLCEVVGWKTGEKRYDKL
                     AKEFTKLLTSSYATTALFGGILLFLLIAFYPKLMNYLTDIFFPSFIVYCLLFLLETVT
                     LYLYWYGWDAMQDGGKKTFHIFLGLLLNVFAFFIMIVPNSWATFQASPVVISEGTDLQ
                     RAWAATWNPTWWPVNIHRLIANVVLGGYICGAYAGIRYLSARTTEDRDHYDWMGYVGN
                     FIGVFGLLPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYY
                     FWLGITHRIPGSEMKYRKPILTMLVILLLCLGVWMTPHSLVASLEEARKMGGTHHPLL
                     GVFGVMSAKMTVANLMILVTFMSFIMYWRAGQEETAGWAKIARALMSAILVLAGIAVV
                     VLGVWGYFVPAIIRINYFSTSQVLIVLFVMLTITPLTALLLKSAKTTTEMVWGRMPPR
                     AGYALVLNAIMVILLMTLMGYARSSSRVHWHIYGVMRDSSQYAFSPALGYAAAFMALN
                     TFCFCMLVAFIFWVATMGDKAKAGHGGAKGDLPQGMPAMAGGSQSLDKQ"
     gene            complement(12351..13313)
                     /locus_tag="VLD60_15180"
     CDS             complement(12351..13313)
                     /locus_tag="VLD60_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46353.1"
                     /translation="MSPTKIGLGVLWPAFWTALPIKLAFVLVFLAMGIMQFEGRIGLA
                     FLMLLASPVTIFALPTISMGLESHIGEGVGIALLFLISIPIDIWALGVVGRTFFLEKF
                     RKEPPDGLGLTLWWKSALVGAIFLPILWFIVSTVTSTSISMAHSFFELEMFKGIPVAE
                     RISIELTIWGSIATAVLLLLLLIGVSLVGRMIRGTASGASPATDNYQGLIKRWDLMRV
                     PADQGLMLAGVTGAGVVLSLLFWAALPVTTPHPHECCKQPEVKVQPPYKPLDALNKDE
                     KMIVQLTAQLEAIEKRKAEEAEKNKGKGKAKDGHEAAPPAAKKP"
     gene            complement(13366..14478)
                     /locus_tag="VLD60_15185"
     CDS             complement(13366..14478)
                     /locus_tag="VLD60_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HSB46354.1"
                     /translation="MEHSSIPSTQPTAIEKVIAFIDERVGLKQMQAKMLNEPVPGGSR
                     WAYVFGSVLLFIFIMQALTGILLMFYYVPTADHAYASTQYIIHEVDYGWFLLSYHFWG
                     STAMVVCVFAHMSQVFLWGAYKKPREMIWLVGLALFGIVMGFGFTGYLLPWDQRAYWA
                     TTVGVEIMDKTPVLGDFMARFLKGGATPGQMTLSRFFVIHVMILPAALMGLAGLHLFL
                     FRAAGPAGPFRGTPEEIKAKTDYFFPRQIWKDIVGMAVVFASICALAFWEPVVLLEEA
                     TPDPGDYHPEPEWYFLFLFQLLRLKIFAGEFGQFLGAIALPGAFMALLAALPFIDKDP
                     ERNIFKRPIALIGWIVVMVAILLFTVSAIINREFLD"
     gene            complement(14479..15411)
                     /locus_tag="VLD60_15190"
     CDS             complement(14479..15411)
                     /locus_tag="VLD60_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSB46355.1"
                     /translation="MQPKLKSRVRCTDREIGEVTKVVVDPLSHEISHIVVSMNGSGER
                     QVAMGHVQAVTDGLVELRAPSSDIAALPPFKRDDYVTTHEVEIAHLEEKIHVTPGEVL
                     VPLPELERSVKRRTFFMNFTHAIGFLIGLPIAYPILRYLMKPMYAPFDNGWLKIGNVG
                     KIKQEDIGVQFKYKKKVKEAYLPEAEIEKNVWILKATPAVLEKVYQGKDMEFRDALGK
                     PVWTNKKEIPYVAFSGKCPHLGCGYKWRQHKLLGQVFLCPCHLSIYDASGKVLDGPAP
                     RPLDALPIRVSAAGEVEIIDMEFKAGTKSQIRIV"
     gene            complement(15414..16217)
                     /locus_tag="VLD60_15195"
     CDS             complement(15414..16217)
                     /locus_tag="VLD60_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB46356.1"
                     /translation="MGNVIKKLVVGLVVGGALIGITRALEFPVIFQMMFFAYAILGAA
                     VFILLDAPSMKTMSGGKALVALLVFYVVLCAVYIAGASMWPQYDPEDEKGKIEKILKP
                     KRAATEQGKAEELIARAKALDEQAKALAERIKALGGDQAPAAQAAGGPGAPPSMAAAG
                     GDILKIGEEQWQLQECYNCHKLRGEGGKKRGPELDNIGSYLTVDDIKEKILDPKSWMA
                     EGFEKEYEKKKMPDKYKDLMEDKDVVALASWLSTFKNTSVNTPKPIKKK"
     gene            complement(16257..>17550)
                     /locus_tag="VLD60_15200"
     CDS             complement(16257..>17550)
                     /locus_tag="VLD60_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46357.1"
                     /translation="ETILLYMYFYTWDAWKGDKKARHIALGVLLNLIGTITLFVIDGP
                     TSFMNTPVKAEGISPQEFLATATLWDKIFNYSWMPLNLHRLVGNVTFGGFVAGLIAAY
                     MYMGAKKEEERAYYDWMGFVGNLIGVGALLFLPFMGYLLAYELCDYDASICPYMMADQ
                     LSMFFEMQGAMIGLIFLASNYYIWLSMKRIEGVEKVRMTILAPIVMVVLPLVMTKVMT
                     DYPVPDPTSLAFLLPLILAPFVLGRFIPLTVSARTVIKIGFLMVVVGDAIWLTPHGFV
                     PTGAKLVAELELPSDWNFLALMPAKNSAAFTLVFVTVVNYVIYNRGISQGTIVWGKID
                     FMSQFVLIFLAFSAIWTMGLMGAVRSLLRKYFHTYNLLPDFTAESFTPTLSYSAWWIT
                     GITVVFYAVVSFAILVTLRPSESKGHVPEGSPVPAGAK"
BASE COUNT         3822 a         5199 c         4859 g         3670 t
ORIGIN      
        1 gagcagggca acgcaagtcc agatgagaaa aaacgaattt tctatggcgg cgttaagatt
       61 tggctgcacg ccaagaaagt tgctcacata gtaggatgac agcgtgacga gctgccctgc
      121 gaggtgactg ggccacgaaa ccggaacaat gacggcggcg gttaaataga ccaggtttag
      181 cccattgtaa tactgcgagg agaagccacc gagaatgagt gtcatttgcg tgacgcagag
      241 gttagccacc acgacccata gaatcacgaa ccaattgggc gggatagatt taggtccata
      301 cttcaggaga aggaatccgc agagtagccc cagttggaga acgacacgga tcatgaagag
      361 cgggatcaaa tattcacggt gttgcagata atccaaagcg atgaaggcag gattcgcgat
      421 gaagcctaag taattcagta cgagcaaacg ctctctgatg aaatttcttc gccattcggc
      481 ataagcttct gctgcctcgc gaccagcagt gtcctcatgg cggatctcgg cagaaaacaa
      541 ttttttccag tcgaaagcgt ttgttaatct ggtccaggac gaactcatgc accatgtcct
      601 caaaaatcgt ggcgcgagaa attactccac cctcacatcg ccgaatagct gtccgctcag
      661 aaccggtagc taatggtgtt gtaaatatcg tagccgctga tcgatgcggt cgcgtttggc
      721 ccgaagggta gtgagggatt ggtgtatgtc acgctgttct tcggggcgta taggccgctg
      781 aagtcgtaag accatctttc agtgaaccgg aatccgagtc caacagaaaa gtggtgttca
      841 tttattaatg gccataaagg gcttagtgtc tcttttggga ttggattatt gctgtagttg
      901 tacccggctc tcagcacaag ccggtctttg tctttccagg acaactcttg catggccgag
      961 tacgagaatc ccaaggctac gacgacctga tctttccaat taaacggaat gttcaagttg
     1021 acttgagagg gagcctgagg gttgtttggg ttcgtcgccg tgatctcaac ggtgttgatg
     1081 gtggcccagt taatccaact cgtcgtcagc gccaccgtga actgctccat cggtttggcg
     1141 gccatcgaca catcgacctg ctggggccac ttgatccccg ccactcgcgc atcatagttc
     1201 accgttccta agccgaatgc cgaaaaattc agagtagcct ggccgccggt gtaattaaat
     1261 ctgacgggcg aggtataggt agctccgacc gtcaaccagg gaagcgcacg gtacatggct
     1321 ccgaccttca ctgaaaacac cacatcggaa gggcaatccc cacccagagg accaaggccg
     1381 ccgtttctgg cacagctgtc tcggatatta aatccggcga acccagcagg taggcccggc
     1441 gaaggcagca cagacgttcc cggaaacaag cgtagcgaga catcggaata ccccacgctc
     1501 ggagcaattc caaaggacag cttttctgtg gcttcatagc ttagtccaat cgcaaacttg
     1561 aggtaccgaa aaaatgacga cgcggcgtcc tgagttccga atgcggtcct gaggtttcga
     1621 taatctgacc caaaccctcc ctgtgtgaaa attcccgcgc caatggtgag acccggcacc
     1681 gaggcaagcc gtgtagcaac cccaaggttc cctaaggcgt acgcgttgtt ttggccaccg
     1741 acattggtat tcccaaagac atccgagtgg tgcagaaacg cctggataaa gcctgctgta
     1801 acatcgaatc gggttccttg gattaatgac atcgcagccg gattggtatt gatggccgaa
     1861 gtgtcggcaa ccgcgacatg accagatgct gccagcgcag tcgattccgc ggagaagccg
     1921 atgaattgag gaccgttgag ggcgtgagca gttcttgtgg tgaggatgtg cgcgcctaga
     1981 acggcgagca ggaggaccca tcttgcgacc gccttcctcg ttgaaaccat cgaaattccg
     2041 cgttgctcgg tgtcgttgtg acctgtaaca caccaaccgc gagtacacga aaaagaaccg
     2101 acaaagttcc tagaccaaag gaagcgtctg gcgtagcgga gccgattcta aaagactcgc
     2161 gtcagacgcg caatagggcc aaatggaata atgaaatacg tgtattaagg ttggtgagtt
     2221 acgctatcgg aatacgtgtg tttgagacac agtactgatc gagaaaaata ctatcccacc
     2281 tctttttgac ctgcggttta ttagtttgct actcctgaaa acgattcaga ctttcaatgg
     2341 gttaatgcgt gctgctacca caaagttacc agcaagtcgg cgtgaaattt caggtagccc
     2401 tacctcagac aaccttagcc cacaagaaac cacaaaaccg gaatcccaca cggttggtgc
     2461 tgactgggct gattcgtcct gccctggtag cagcgtggta gcagaaagtc atcaagatga
     2521 tcctgagtcg agcccgctgc ttaagagtcc cttcgaaaac ctagctcagg acactcagca
     2581 agaaccatca tcaacaaaat gtgagaattc gtgatggcgc agaccatccc gggtcatggt
     2641 gtgtcaggat gttttagcac caagttggca ccacctaaca tgacattgtt cgattgcgag
     2701 ccgaacagca actcaaacgg ctccgccggt ttgacatgcg ctgcgggcgt ctcatcctca
     2761 ccgactagac ggacctgtta catgctgaag agcggtcatg ggaccgaacc ttcgcagcgt
     2821 ggcgagttgc cgcctgtcct tgacgaggaa cgccccggca aaccccaagg cattgaccga
     2881 gatgccctcg aagcattctt gcgaccgtgg cacaaccaac atccaccttc gagtcacgag
     2941 caggttgtac ggagcggtca gcgcatgact gcccgtggtt gcgtcaacag acaggccggt
     3001 cgcgtgaagc aatttccggt agcaggcatg caaggaagcg ccgctgtcct tttccggatc
     3061 ggtccagtct cgatccattg gcgcataggc atggcggaac ggcaggcgcg gtacggtacc
     3121 gatcatgccg tccgtctgag cgaagcgcaa gaatggctcg atgggtagcg gaccaatcgt
     3181 cgcgtcgaac ggcaccaatt gcaaatgttt gtgcgactgg ctggctccag cctcgtgtcc
     3241 cgcgttgtag aaaccgaggc cgtcgatctc ggtgagcccg atgaggagcg cctcgcagtc
     3301 tttttgtgtg agctgacatc gctgatcctc gaatgctctg gtgacgatca acaggtggcg
     3361 ttcgaccacg ttgaacttgt tcagcaaggc gatgtgggtc ggcgaaattg gcgcgacgaa
     3421 gaggtcggga tcatacggaa gaaacggatt gacattcgat cgagccgctt tggctatctc
     3481 tttcatggtc agggattcca gaacccgcac ctggaacggg atgccgtcct gctcgatcac
     3541 ttccacagtg gtcggcagga attgtatggc gccgcaccgg cgggcgtggg ccgtacgttc
     3601 caacagggag gcctgcaacg tgcctggtgt cagatgcaaa tcctgatcag ccatacaaaa
     3661 gaatggtacg ccaagcgaag ggggcgagaa aagagagacc gaccgtgagg caggcaaggc
     3721 taaaagtgga attccgctga tgtagagtca gcggcgagct ttccttgata ttctcctacg
     3781 ctattcacta cgccgtcctg accgtcgtcg caaaggccgt tgcaccgtcg cgctaagtct
     3841 ttgaaagtta gtgagaaaag aatggagccg gcgacccgga ttgaacgggc gacctgcggt
     3901 ttacgaaacc gctgctctac caactgagct acgccggcgc cgcacgagat cccataacct
     3961 acacgggttt tttctcagcc tcaaggggtt tttgcatggg atgcgggttg agggaggcct
     4021 ttttctcctg attacctatt tcagtttcgc aaggggctgc aaacgaacca tggccccttg
     4081 gcctggccct gggagacaga gaggctcctg gtcgtcgccg aactgttcgc gggccatttt
     4141 cgcggcccgt cccctgctgt aggcgcagag ttccccagca aagatcgttc cgtccttatc
     4201 aatgtcggct gccccaccca atccgcgcag caagtggtat gtgtatagac tgtgctggcc
     4261 gagatcgtag gcgtgggctt cctgtaccgc cctgtttccc accatccaca taatcttgtc
     4321 tttgtcctgg ccctccattt cccagagcgg ggcggcatga gttgccccct ccggaagctg
     4381 accatattca agggacagat cgagcatgat gatcgctcga tgaatcggca gcttgaccag
     4441 agattcctgt aaacgcctga gcgaatagac gcgagcgcca gacgacgccg tgccatcgaa
     4501 tgggatcacg gacacagctc cggtcgtcgg ctccacgatc ccgcggccgg agatatagac
     4561 atagacgacc gtcgtcggat cgacgtgctt gggaagccat tcttcaaaga cctcggccac
     4621 atcggttttg agcgcatggc tatcgatgag ccggcgtatc cggtcagggg ggataccgcc
     4681 gatggcgctc cagtacttcg ccatgacctt ggcatcttga gccgcaaact tgaccctggg
     4741 caggccagtc tcacgatact gtccgatccc aatggcaatg ccaaccgcct tgggctgctt
     4801 gagctttcca gaatttttgg gaacctcatc gacgtcaacc ggaaccatcg cggcctctgg
     4861 cgcatcggcc ggttttatgg ccaccaaaaa ctttttggct gctgggatat gcgcacctcc
     4921 agactgactc cgcagcgcta gaaccagctc tgcctggccc gcctcggtaa cggtcccgac
     4981 tttcccatcc agggacacgc gtttcacatc accagcgggc agatctccaa cgggcagtac
     5041 gcttggaata tgctcgacca gcgccggcgt gccactgacc agcacttcca cgcccgacgc
     5101 cgatcctggt ccttcgttct tgacctcgat ctcgacccca agggtttcac cggaatggag
     5161 caactgattc cggttctcgt cacggatgat ggctcgaaac accagcgtcg acgcctcgtc
     5221 ggccgccggc ggcggcacag gagccgtgtc tttcggttga gccgtcacag cctcatttgc
     5281 gcctcccgct gcgatcggcg gaggcggaac gacccccccg gttcgaggtg caccggtttt
     5341 gggggcctca gccgccatga gaatcttact ggaggtgccc agctgtttgg ccatcccgtc
     5401 ggtgacattg ctgatggcct cttgcgcgag cttgtcgagc cctttgactt cacaagaaga
     5461 ctcggtcacg tcgacctcgc cacgcccgac actttgaagt ttcttggaga agagcacggt
     5521 cccgtccgcc gccgtatagg cgaagtccaa tccaatggcg agtgtcgccg ggtagcttct
     5581 gttggccttc tgcgaaatca caaggtccag cctggtcatt cccaacgaaa catcctcgta
     5641 gccgtcgacg gcaattgtac cgggcgcatc gatcaccacc ttttcgaaga ctcgtccgct
     5701 cttgcgtttg accgcctctt caagcatggc accgatcggc agggatttcg gatagccgca
     5761 agcatcctga tactggacct gtgcggcagc gatgctcgag gcgggacgca actgcaccgt
     5821 gagcggcaaa tagtaaccga tccctgcccc atcgcgactg gagaaaagcg cacaaccggt
     5881 gagtgcgagc gaaacgatcc agacacacag tgaaccagcc ggccgtctgg agataatggg
     5941 gagccaacca tgaggcattc gcacaatgat ctttataccg aaattcatcc ggcgatcaca
     6001 actcaagggc agttcgatgc aattgacagg tcgcggagcc tcggctacgt tggctccatg
     6061 attgcgccgt cggccccgcc tcacgacgtc ccatctcagt cgaatttctc ctggtccgcc
     6121 gtcacccgac tgatacggct gcggaatcag accggaactt ggcttctgct gctcccgaca
     6181 ctgtggtcgc tcatcctcgc tgcgcgaggc attccaccct ggttgctcat cgtgattttt
     6241 tctctcggtt cgtttctgat gcgaagcgcg ggggtggtcc tgaacgatct cgcggaccgc
     6301 ccatttgatc ggcatgtgac gagaacccaa cagcgcccct tggcttccgg agagctgcgg
     6361 ccatggcatg cgctggtcgt ggtcggcatc ctcctcgccg gagccggcct cctcgtgctg
     6421 ttactgaact cgtttacgat catgctgagc cccatcgcgc tacttctggc cgggctctac
     6481 cccttcgcca agcgagtggt ccagatccca caggcgatgc tgggcattgc attcggttgg
     6541 ggaaccatca tggcatgggc cgcttcggac ggcacgatcg aggctccagc ctggttggtc
     6601 tttgccgcta cggtctgctg ggccgtcggg tacgatacga tttatgcgct gcaggaccga
     6661 gaggacgacg aacgcatcgg cgtgaagtcg tcggccctct tcttcggatc ttctgcctgg
     6721 ctggcggtcg gagtcagctt catcgcgatg gtcgcactat tggggctggc gggatggctg
     6781 acgcaaatcg ggtggttctt ttatgcggtg cttgcaggag tgagcctgta ttgtgccacg
     6841 caggccctga agctcagaaa ccctgttgcg ccgttgccgg ccttcgacat gtttcgtcga
     6901 catgtctggg tcgggtccgc gatcttggtc ggactgatcg gcggtttctt agtttagtcg
     6961 ggccaacagg ctgctcaaaa aagccattac tctcacccgc ccagccccga tgcgccaaga
     7021 cgcaccgttc cccaggcgcg gccgcagcga acgaagaggc gagaggcata ctctgttctg
     7081 tatgtcgagc ctgtgagtga cgcgagaacg acgctggagg actgtttcag cagcctgctc
     7141 tcagctcccc tgtttgtcgg cgggcggttc cgccttcggc gcgcgcaacg tgctcagata
     7201 catggacacg gccttcgcat ccgcatcgct gaggcccaat gccggcatcc tggtttccgg
     7261 tttcatggcc tgggggttct tgacccagcg gtacacccac gtcgaattca aacggaaccc
     7321 cgcgcgatcc aaggccggac cgactttacc accttcttca ccgaggctgt ggcaaccgtt
     7381 gcaaccgtac ttattcgagt agagccgctt cccgacctcg gccaattgcg cggcctgctc
     7441 gggtttcatc gtcagatcag gcctggccac gttcacgcct ttccctggcc tcgtcgagaa
     7501 attgttcagc gctgcttgag cggtatcaag ctcttttttc gcctgcgtca tggccgtctg
     7561 ttccttcgca taggaggatt cgtcgacttc gacgtccttc ttcagcgctt cgcttttctt
     7621 ttctgcttcc tcgctcgctt tcttttccgc cgcctcgtag gtcagcttgg cttggtcgaa
     7681 cttctcctgc gccgtcttca atgcttcggc cagttctttc ttgcgctctt cgactttgaa
     7741 ctctagcttc attccatgca gggtccggac gtagccgaca atcgcccaga tttcgtcttc
     7801 cgaaagggtg tacttgaagg tcggcatcgt cggcacggca aaatcatcgt cgccgatctt
     7861 gtcgccgccc gggctcgtgt ccttcatgtc gcgtgaaatg gtattgaaaa tgtcctcgtc
     7921 cttgaaggtc cccatctcgg acttgttgga gagatctttc ggtttgggat ccggcatcga
     7981 cgaccagttg aagccctcgt tctgccgccc attctctccg tgacaatgca tgcagtaatg
     8041 ggcgtaaagt tcctgcccct tgcgctgctg ttcgctcgca cagcccccgg cgaccatggc
     8101 ccatacaccc atgaggccag ccaccgctcc tatgacttgc agtcttgccg ccttcatgct
     8161 gactgccctt tcttcagttt gcggatcaac acgaactgaa acgccagcgc caaccccatc
     8221 gcgagcgccg cgtacaaata gcccgaatag tcgggcggcg cttcggcgcg gaaataccac
     8281 caggaggaca cggctttctg agaccccttc tccacgaggt cgccggctcc gtcttttctt
     8341 ccgtcccaca cggcaaaggc gacattgatg aactcgcccg gcttgacctg cgtatcttca
     8401 tccggatggt cggtcgaaag attccgcgag aagacgactt tccagcttcc attggcgtag
     8461 acacccttgg ccttgacgtc ctgatggtcc tgcgctttca aggttccgaa acccttggcg
     8521 ctcatgtcca cgcctttatt gtctttgttc ttccaatacc aaatgttgac cgggccgcct
     8581 tccacttgag ccatcggctg gccatgggca aagtgggcct tcttgtcgcc gaccatgaat
     8641 tccagcgcgg cggcatcgtc agggttctcc gtcggatcgg cgtactccag gagcaccgcc
     8701 acctgcgtcc catcgtgaag cgcccggact ttcaaatctt tgacggtcac ttcctggata
     8761 cgattttgcc aatgcacctg gggagacatc gggaattccg cctgcggcgc gctgttccag
     8821 gctgcagcgt tggggtcatc gaccgggagc gcgcccttga ccatcaaggc ccttaccgcg
     8881 acgctttcct gggccaggct gaccgtcgtt ccagcgacga acaacgctat accgaatcct
     8941 gcgaccgtgc tgccccactg tattccttgt ctcattcccc gtgtcatacg agcctcgttg
     9001 ctagttcgac ttacgctcag ccatgcgatc ggttattccc aggttcttgg agattgatgg
     9061 gccccgtcgt attcacccat gatgatcttc cagatccatt catccggcag ttcgacttcc
     9121 catcgaggca tcgcggattt ccagggcatg ccttcgatcg gcaagccaac gccgcccttc
     9181 ttaatgcgcc agaacaagta gctttcctga agcatcgcga tcgtggtcgg atccgagaag
     9241 ttcgccggcg gcggattgaa gccccgagcc gcaggaccct tgccgtcgaa gttccccccg
     9301 tggcagggcg aacagaacgc ggcgtagaac cctttcccct gcatgatgtt gtccggcgtc
     9361 ttcgcgacgg gattcgacag gcccgtgtat tcgcccggag gcgccgggtg gatcgtgcgg
     9421 ttctcggcgg gaggcgcatc gctgacggcc gtgctgctgt atgtctgcca cccgacaagc
     9481 aacgggaata caatcaacac tgcataccgt gccgcttgca acgcgccgat gttttcaccg
     9541 gtcaggaacc gctggatcgg gccccagaag gcgtctttcg attcgccgct catcgtcgca
     9601 aaaatgacga tcccggacgt tgtgagcatg atgtagagga aaatcagact cgaaggcaac
     9661 ggcgcggacc cggggatatt gggcacgacg aatttcagaa tcagatagac gacggcgatc
     9721 aggattgccg gtttggtcaa gccacccata ggtgcctcct cagtgcgctc gtgcgacggc
     9781 taacggaacg gattcagccg ctttcaccgg ggcggcggga gcctgacccg gcacttccaa
     9841 ttcggacgga ttgactgaaa tcggctcccc gctcatctgc tgcaggaacg cgatcacgga
     9901 cagaatctcg ttcttggtga gcccgatcgg gttcttattg atgtacggca tcctggccgg
     9961 atactccttc ggcaatccct catgccgata gtcgagatag acgtacgcct gcggttgcgt
    10021 caggctctcg aaaatgaatt cgcggctcaa cttggcaccg atgcctttga gatcggggca
    10081 gcgagccgat tcgctcgggc cgattgaatg gcagagcgcg cattggcctt tgctgaagaa
    10141 aatcttctga ccgatcgccg ccatgtcggt cggcgtcttg acggtggcaa tgtcgaactt
    10201 ctcttcagcc ggcggcagcg aggccatctg gggcacagcc agggcgacga gcgaaaagag
    10261 tcccaggacg atcgcgacga atccgatgac acgggcgaat tgtgcccgga caaacagaag
    10321 ggtgacgatc ccgaatccga caaccgagag tccgatcagc tgcagcttga cgacttcact
    10381 cataatccct cgatatgata cgcaataact gcttgccttc agctctcagc tttcagcaat
    10441 cagtcaaaac tgaccgctga tggctggccc ctgacggcta ttgtttatcc aacgactgcg
    10501 accctccggc catcgccggc attccctgtg ggagatcgcc cttcgcaccg ccgtgaccgg
    10561 ccttagcctt gtcgcccatg gtcgcgaccc agaagatgaa ggcgacgagc atacagaagc
    10621 agaacgtgtt cagcgccatg aacgcggcgg catagccgag cgcaggagag aaggcgtact
    10681 gagacgaatc acgcatgaca ccgtaaatgt gccagtggac gcgcgaggag gacctcgcat
    10741 agcccatcag cgtcatgagc aggatgacca tgattgcgtt gagcaccagc gcgtagcctg
    10801 cgcgcggcgg catccggccc cacaccattt ccgtcgtcgt cttcgcgctc ttgagcagca
    10861 gcgcggtcag cggcgtgatc gtcagcatca cgaacaggac gatcaacacc tgcgaggtcg
    10921 agaaataatt gatgcggatg atggccggca cgaagtagcc ccacacgccg aggacgacaa
    10981 ccgcgatgcc cgcgaggacc aatatggcac tcatgagagc cctcgcgatc ttggcccacc
    11041 cggccgtttc ttcctggccc gcgcgccagt acatgatgaa gctcatgaag gtcacgagaa
    11101 tcatcaggtt cgcgacggtc atttttgccg acatgacgcc gaacacgccg agcaacggat
    11161 ggtgcgtgcc gcccattttt ctcgcttcct ccagactggc gacgagcgag tgtggcgtca
    11221 tccagacacc gaggcagagg agaagaataa ccagcatcgt cagaatgggt ttgcgatact
    11281 tcatctcgga gccgggaatt cgatgggtaa taccgagcca gaagtaataa ttcgagccaa
    11341 ggaagagcac accgatcagc atggcttgca ggatgaagag ccaggagagg aatccgccca
    11401 taagcgtgat gcccatctgc tggttgtact ggtagatttc ccgcatcagc caatagccgg
    11461 cgaagggaag cggcaacagg ccgaaaacac cgatgaagtt cccgacgtag cccatccagt
    11521 cgtaatggtc gcgatcttcc gtcgttcttg ccgacagata gcggatgccg gcataggcac
    11581 cgcagatgta cccgccgagc acgacgttcg cgatcaggcg gtgaatgttg accggccacc
    11641 aggtcggatt ccaggtcgca gcccaggctc gctgaagatc cgtgccctcc gaaatgacga
    11701 caggacttgc ctggaatgtc gcccaggagt tcggcacgat catgatgaag aacgcaaaga
    11761 cgttgagcag caggccgagg aagatgtgaa acgtcttctt cccgccgtcc tgcatcgcat
    11821 cccagccata ccaatagaga tagagcgtca cggtttcgag caagaagagc agacagtaga
    11881 cgatgaatga cggaaagaaa atgtccgtca gataattcat caacttggga tagaacgcga
    11941 ttaacaagaa gagcagaatg ccgccgaaga gcgcggtggt ggcatacgat gaggttagca
    12001 gcttcgtgaa ctccttcgcc agcttgtcgt agcgtttctc accggtcttc cacccgacga
    12061 cctcgcatag ccaggcgaaa atcggaacgc ccaacacgaa accagccaga aggaggtgca
    12121 actgcgcgat gatccacacc agattgcggc tgccgatccc cgggatgtca cgatattcgg
    12181 tgggaccagc cggcgcgcct tctgcggcaa tgccgatcga agggagagcc aaccagcccg
    12241 cggcaagcaa cagccccggg agccatccgc catgaccgct gagggtctgg agaatccgct
    12301 gaatcaatcc gccctggtgt cgcatggcac tcacctcatc atctcgcaga ttacggtttc
    12361 tttgcagcag ggggagccgc ctcgtgaccg tccttcgctt tccccttgcc cttgttcttt
    12421 tcggcttctt ccgccttccg cttttcgatc gcctcgagtt gggccgtcag ctgcacgatc
    12481 atcttttcgt ccttgttcaa ggcatcgagc ggtttgtagg gcggttggac cttcacttcc
    12541 ggctgcttac aacattcgtg cgggtgcggt gtggtcaccg gcagcgcagc ccaaaagagc
    12601 aatgacaaca ccacgccggc acccgtcact cccgccagca tcaggccttg atcagcggga
    12661 acacgcatga gatcccagcg cttaattagg ccttgataat tgtccgtagc gggactcgca
    12721 cccgaggcgg tgcccctgat catgcgaccg accagcgaca cgccgatcaa caacaacagc
    12781 agcagcaccg ctgtagcgat cgaaccccaa atggtcagct caatgctgat ccgctccgct
    12841 accggaatgc ccttgaacat ctccaactcg aagaacgaat gcgccatgga aatcgaggtc
    12901 gacgtcacgg tactgacgat gaaccagaga atcgggagga agatggcgcc aacgagggcg
    12961 gacttccacc ataacgtgag cccaaggcca tccggcggtt cttttctgaa tttctcgagg
    13021 aagaacgtcc gaccgacgac cccaagcgcc cagatatcga tgggaatcga gatgaggaag
    13081 aggagtgcga tcccgactcc ttcgccgatg tgcgactcca agcccattga aatcgtcggc
    13141 agtgcgaaga tcgtcaccgg gcttgcgagc aacatcagga atgccagtcc gatgcgtccc
    13201 tcaaattgca tgatgcccat cgcgaggaac accaacacga acgcgagctt gatcggaagc
    13261 gcagtccaga aggccggcca gaggacgccg agtcctattt tcgtcggaga catggattca
    13321 cgttcgtcag tcatgatcag tacgggcggc tcaacgacgc cgggcttaat ccagaaactc
    13381 tcggttaata atcgcggaca ccgtgaacaa gaggatcgcg accatcacga cgatccagcc
    13441 gatcaaggcg atcggacgct tgaagatgtt ccgctctgga tctttatcga taaagggcaa
    13501 cgcagccagc aatgccatga acgcaccggg cagggcgatc gcacccagga actgaccaaa
    13561 ctcaccggcg aaaatcttca accgcagcaa ttggaacaag aagaggaaat accactctgg
    13621 ctccggatgg tagtcgccag gatcaggggt ggcttcttcc agcaacacga ccggctccca
    13681 gaaggccagc gcacagatgc tggcaaatac caccgccatg ccgacgatgt ctttccaaat
    13741 ctgccgcggg aagaagtaat cggtcttcgc tttgatctct tcaggcgtgc cccggaacgg
    13801 accggctggt cctgctgcgc ggaacaagaa gaggtgcaat ccggccaatc ccatgagagc
    13861 ggcaggcaga atcatgacgt gaatgacgaa gaaccggctc aaggtcatct gtcccggcgt
    13921 cgcgccgcct ttgaggaacc gagccatgaa gtccccgagc accggtgtct tgtccatgat
    13981 ctcgacaccg accgtcgtcg cccagtaggc acgctggtcc cacggcagca aatagccggt
    14041 gaacccgaac cccatgacga tgccaaagag cgccagacca acgagccaaa tcatttctct
    14101 cggttttttg tacgcgcccc agagaaagac ttgcgacata tgggcgaata cgcagaccac
    14161 catcgcggtc gaaccccaga agtggtaact caagagaaac cagccgtaat ccacttcgtg
    14221 aatgatgtac tgggtgctgg cgtaggcgtg atccgcggtc ggcacgtagt agaacatcaa
    14281 caagatgccg gtcagggcct gcatgatgaa aatgaacaac aggacggacc cgaacacata
    14341 ggcccaacgg gagccgccgg gaaccggctc gttgagcatc ttggcctgca tttgcttcag
    14401 cccgacgcgt tcgtcgataa acgcgatgac tttctcgatc gcggtcggct gcgtgctcgg
    14461 gattgaagaa tgttccattt agacgatccg aatctgcgac ttggttcccg ccttgaattc
    14521 catgtcgatg atctcgacct caccggcagc cgatacccga atgggcagcg catcgagcgg
    14581 acgcggcgcc gggccgtcga gcactttccc cgaagcatcg tagatgctca agtgacaggg
    14641 gcagagaaac acctggccga gtagtttgtg ctgccgccac ttgtatccgc acccgagatg
    14701 cggacacttt ccggaaaatg cgacgtaggg aatctctttc ttattcgtcc agacgggctt
    14761 gccgagggcg tcgcgaaact ccatgtcctt gccctgatag accttctcaa gcacagcggg
    14821 agtggccttg agaatccaga cgttcttctc gatttcggcc tccggcaggt aggcttcctt
    14881 gacctttttc ttgtatttga actgcacgcc gatatcttct tgcttgattt tgccgacgtt
    14941 cccgatcttg agccatccat tgtcgaacgg cgcatacatc ggcttcatga ggtaccgcag
    15001 gatcggatac gcgatcggca gcccgatcaa gaacccgatg gcatgcgtga agttcatgaa
    15061 aaacgtccgg cgtttgacgc ttctctccag ctcaggcaat ggcacgagca cttcgccagg
    15121 cgtcacatga atcttctctt ccagatgcgc gatttcgact tcgtgggtcg tcacataatc
    15181 gtcgcgcttg aacgggggca acgccgcgat gtcggatgag ggggcgcgca actcaacgag
    15241 accgtctgtg acggcctgca catggcccat ggcgacctgc cgctctccgc ttccgttcat
    15301 ggacacgacg atgtggctga tctcgtgcga taggggatcg acgacgacct tcgtcacttc
    15361 accgatttca cggtccgtgc agcggaccct ggatttcagc ttcggctgca tcgttacttc
    15421 ttcttgatcg gcttcggcgt gttcaccgag gtgttcttga aggtgctcag ccaggaggcg
    15481 agcgcgacga cgtccttatc ctccatgaga tccttgtact tgtccggcat cttcttcttt
    15541 tcgtattctt tctcgaatcc ctcggccatc cagctttttg gatctaaaat cttctccttg
    15601 atgtcgtcga ccgtcagata gctgccgatg ttgtcgagtt cgggaccgcg cttctttcca
    15661 ccctcgcctc ttaatttatg gcagttgtag cactcttgaa gctgccactg ctcctcgccg
    15721 attttgagaa tatcgccgcc tgcagcggcc atagacggcg gggcaccagg gcctccggct
    15781 gcctgcgcag cgggagcctg atcaccgccc aatgccttga tccgctcggc cagagctttc
    15841 gcctgctcat caagcgcctt ggctcgcgcg atcaattcct cggccttgcc ctgctcggtc
    15901 gcagcccgct tcggcttgag aatcttttcg atctttccct tttcatcttc cggatcgtac
    15961 tgcggccaca tcgacgcgcc ggcgatgtac accgcacaga gcacgacgta gaacaccaaa
    16021 agggcaacga gagccttgcc tccgctcatg gttttcatcg acggggcgtc cagcaggatg
    16081 aacacggcgg cgccgagtat cgcataggca aagaacatca tctggaagat gacgggaaac
    16141 tcaagcgctc tcgttatacc gatcagcgcg ccacccacca cgaggccgac caccagcttc
    16201 ttgatcacat tccccataca ggtcctcttt ccttcagtga ggatttcacg ccaggctcac
    16261 ttggcccccg caggaactgg actgccctca ggcacatgcc ccttcgactc agaaggccgc
    16321 agcgtcacga gaatggcaaa actcacgacc gcgtaaaaga cgacggtgat cccggtgatc
    16381 caccaagccg aatacgacag cgtcggcgtg aaggactcgg cggtaaaatc cggcaacaga
    16441 ttgtacgtgt ggaagtattt ccgcagcagc gaccgcacgg cacccattaa tcccatcgtc
    16501 caaatggcgc tgaacgccag gaaaatcaag acgaactgcg acataaagtc gatcttgccc
    16561 cagacgatcg tgccctgact gatgccccga ttgtaaatga cgtagttcac aacggtgacg
    16621 aacaccagcg taaaagcggc ggagttcttt gccggcatca acgccaggaa attccaatcg
    16681 gaaggcaact ccaattcggc gaccaacttg gcgccggtgg gcacgaatcc gtggggggtc
    16741 agccagatcg catctcccac gacgaccatc aagaagccga ttttgatcac cgtccgcgcg
    16801 gagacggtca acgggataaa ccggccgagc acgaaagggg ccaggatcaa gggcagcaag
    16861 aacgccaagg atgtcgggtc aggaaccgga tagtccgtca tcaccttggt catcaccaat
    16921 gggagcacca ccatcacgat cggcgccagg atcgtcatcc tgaccttctc gaccccctcg
    16981 attcgcttca tgctcagcca gatgtaatag ttgctggcca ggaagatgag cccgatcatc
    17041 gccccctgca tttcaaagaa catcgagagc tggtcggcca tcatgtacgg acagatcgac
    17101 gcgtcgtagt cgcacagttc atacgccaag agatagccca tgaacgggag gaacaacagc
    17161 gcgccgactc caatcaggtt gccgacaaac cccatccagt cgtaatacgc ccgttcttcc
    17221 tctttcttcg cgcccatata catgtaggcg gcgatcaggc ccgcgacgaa accgccgaat
    17281 gtcacgttgc cgacgaggcg gtggagattg agcggcatcc aactatagtt gaaaatttta
    17341 tcccacagcg tcgcggtggc aagaaactcc tgtggcgaaa taccttccgc tttcaccggg
    17401 gtgttcataa aggaggtcgg tccgtcgatc acgaacagcg tgatcgtgcc gatcaagttg
    17461 agcaatacac ccaaggcgat gtggcgcgcc ttcttgtccc ccttccatgc atcccatgta
    17521 tagaaataca tgtagagcag gatcgtctcg
//
LOCUS       DATIFR010000263         5650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_957700, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000263 DATIFR010000000
VERSION     DATIFR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957700"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VLD60_15205"
     CDS             complement(<1..861)
                     /locus_tag="VLD60_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB46358.1"
                     /translation="MRARILIVDDDPDIVLALENRVTWMGHEPLIADNGKDGLRLIQQ
                     EQPDLVLLDIQLPIFSGLEILQQIHDLSRTEGSGYGQVGLAAGPLIPPIIMLTAFGTI
                     ELAVKAMQLGAMDFITKPFTGDHITVVINKALDAISLRRQVDVLQQEVDERYGPIVGD
                     NAKFAAQLALAKHAASSNVTVLILGETGTGKEVVARAVHHWSPRKSKPFVAVNCAAIP
                     KDLLENELFGHEKGSFTGAVKREAGKIEIAEGGTVFLDEIGDMPLTLQSHLLRLLQDQ
                     TFYRVGGTQPV"
     gene            complement(947..3271)
                     /locus_tag="VLD60_15210"
     CDS             complement(947..3271)
                     /locus_tag="VLD60_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB46359.1"
                     /translation="MAHSPSSNALDDPMNAGKSRFLSLRTKFVLFFSLILIVSCSTLS
                     WYLIENRRAAMAENLRQLGTILLTNVVGNEHFRYAGLVAQDRATLKEFVDGLMAVQDV
                     VYVVVRGPDGAVITKETKGGRRSPASLLRSPEYPLYPDESIAHTLLSTPHSAPKMTPL
                     SIVNVVSPRFAWEERVYDFAMPVLRTTPSGGSIAPFSLQLEERSQPRPSPDGPQVSGL
                     VQIGLSDANLNHELLSMVTNVILLTVVIIAAGILGAHILASKITTPLRGLAAVAHQVA
                     EGKLPEPLLSSTRDEVGELTHMFNVMTQALNERNLAITANMDTIKRQLGQLTTVHQTS
                     AAITSTLNLHELMDTVLQLLMTNLGLSRMVLMLRHEDRDVAYVAQVAGVSDEIADAAR
                     HLTIPVKEDNSLMADLLIHAKPVLIHDITTIAHRMHPAILELASRVGVTSFVLVPLQT
                     HNQILGFLGGDRGAQPCTEEDLHILLTIASHVATAIDNARTYADLSQLTQTLEYRIQE
                     RTQELLIANEQLQEHDRRRTMFVSVASHELRTPMTAIRSFADNMLDGVAGALTERQKT
                     YLDRIEHNLNRLTRIINQLLDWSRLDMRREVLRIEPLCIKQISTLVVESLRTVAAEKN
                     VAIDIEAPEQLSMVYADRDKLEQILWNIVGNAVKFTPPGGRVSVTFEPLPDDTIQVCV
                     ADTGCGIPPEHLDAIFHEFSKVPSAMPSAQGAQLGLFITKSFITMHKGRTWVQSTLNS
                     GTRFFFTLPCASEQGQASSMSPQMVEPPSATKTS"
     gene            complement(3279..4238)
                     /locus_tag="VLD60_15215"
     CDS             complement(3279..4238)
                     /locus_tag="VLD60_15215"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB46360.1"
                     /translation="MTRRANRLRTGSCRAPRLVLGFAVLLSLFALCDGESMAMDIAIL
                     KSSGINAYEQAIEGFKATAPSGIIYTEYDLQGDLEQGRKLARKIRASDTSLVVAVGLK
                     AALAAKLEIVDTPVVYMMILDPLKHYLNASNMTGTLLEIPAERQFKILRAFLPNLRRL
                     GSLSSPGNSSGKFKEATAQASAQGFTLQDFPVESEKEVPQQLRSLLSTVEGLWLIPDS
                     KVLTNESIGFILESSLARRIPVIGFSPEFTRLGALLSLSVSYGEVGRETGLLAKRILD
                     GDKKLPAKPVPIERLTITVNLKTARFLGIEFPKEVNHLIDETY"
     gene            complement(4282..>5650)
                     /locus_tag="VLD60_15220"
     CDS             complement(4282..>5650)
                     /locus_tag="VLD60_15220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSB46361.1"
                     /translation="KDCPVHASLRDGVVYRVNDEVFWRKDGTSFPVEYISTPIREDDR
                     VVGVVMVFRDTTDRKKAESALRASEERLELVIRGSNDGFWDGQVLPGKPWNSPDTPIW
                     WSSRVREMLGYTEEEFPDVLKSWASRLHPEDRERVFAALTAHIERRVPYDEEYRLLTK
                     QGDYHWVRARGQAIWDAVGRMIRMAGSLQSIMDRKRTEEAIRRSQQLLQEVADTTTAV
                     IYAKDPDGRFLFVNRRFEELFNLTADQIIGHTNHEIFPRKIADAFRANDIEVFEKNAT
                     VEYEEQAPHQDGLHTYLSIKFPLRGQDGSPYAVCGVSSDITERKLAEDALRTHEAQLR
                     HALTSAEVGGWDWDRQTGQFCWSRQVDTMLGLSDGSRARSREDWLALVHPDDRQAMAQ
                     ALDEALEQGGQDVLFEHRTVTTDGSVRWLVWSGQVLRNREGKVVHMLGMVRSLSARPR
                     KTREK"
BASE COUNT         1126 a         1589 c         1737 g         1198 t
ORIGIN      
        1 gaccggctgc gtgccgccga cccgatagaa ggtttgatcc tggagcaggc ggaggagatg
       61 gctctgcagc gtgagcggca tatcgccgat ctcgtcaagg aacaccgtgc cgccttcggc
      121 gatttcgatt ttaccggctt cgcgtttcac cgcgccggtg aatgaaccct tctcgtgacc
      181 gaacagctcg ttttcaagca ggtccttcgg gatagccgca cagttcacgg ccacgaacgg
      241 tttggacttg cgggggctcc agtgatggac ggcgcgggcc accacttcct tgccggttcc
      301 ggtttcgccc aggatcaaga cggtgacgtt cgatgaggcc gcgtgcttgg ccagcgcgag
      361 ctgggcggcg aacttggcgt tgtctccgac gatggggccg taccgctcgt ccacttcctg
      421 ttgcagcaca tcgacttggc ggcgcagaga gatggcgtct aacgccttgt tgatcaccac
      481 cgtgatgtgg tcgccggtaa acggtttggt aatgaaatcc atcgcaccga gttgcatcgc
      541 tttcacggcg agttcgatcg tgccgaacgc ggtgagcatg atgatgggag gaatgagcgg
      601 tccggcggca agaccaacct gtccgtatcc cgatccctct gtacgggaca ggtcgtgtat
      661 ttgttgcaag atttccagtc ctgaaaagat cggcagttga atgtccagaa gcaccagatc
      721 cggttgctcc tgctggatca gacgaagtcc gtccttgccg ttgtcggcga tgagcggctc
      781 atggcccatc cacgtcactc gattctcgag tgccaggacg atgtcgggat catcatcgac
      841 aatgaggata cgagcgcgca tgcgaacgtc ctatccgtga gaacgatgaa gaaagtctag
      901 accatttgtc cgggaaaata tatgtcccgg gttcatcctc caggcctcat gaggtcttcg
      961 tcgccgaggg cggttcaacc atctggggcg acatcgagct tgcctgaccc tgctcggagg
     1021 cgcagggcag cgtaaagaaa aagcgcgtgc cggaattcag cgtgctctga acccaggtac
     1081 ggcccttgtg catcgtaatg aagctcttgg taataaacag tcccagttga gcgccttgag
     1141 cgctgggcat cgccgacggg actttcgaga attcgtgaaa aattgcgtca aggtgctccg
     1201 gaggaatgcc gcagcctgtg tctgccacgc acacctgaat cgtgtcgtcg gggagcggct
     1261 cgaaggtgac cgacacacgc ccgcccggcg gggtaaactt tacggcgttc ccgacgatat
     1321 tccaaagaat ctgctcaagt ttgtcgcgat cggcataaac catcgacagc tgctcaggcg
     1381 cctcgatgtc gatcgcgaca ttcttttcag cggcgacagt acgcaggctc tccaccacca
     1441 gagtgctgat ctgtttgatg cacaacggtt caatgcgaag cacttcccgt ctcatgtcca
     1501 atcgtgacca gtccagcaac tgattgatga tcctggtgag ccgattaagg ttgtgctcga
     1561 tgcgatcgag ataggtcttc tgacgttccg tcaaggcgcc ggcgacgcca tcgagcatgt
     1621 tgtcggcgaa gcttcgaatg gcggtcatgg gagtgcgcag ctcgtgggag gccacggaga
     1681 cgaacatggt tcgcctccgg tcgtgttctt gcagctgttc gttggcgatc aagagctctt
     1741 gtgtgcgttc ttgaatgcga tactccagtg tttgcgtgag ttgtgagagg tcggcatagg
     1801 tcctggcgtt gtcgatcgcc gtggcgacat ggctcgcgat ggtgagcaga atatggagat
     1861 cttcttccgt gcagggctgc gcgccccgat cgcctccgag gaagcccagg atttgattgt
     1921 gcgtttggag cgggacgagg acgaaggatg tcacgccgac acggctggcg agttcgagaa
     1981 tggcggggtg catgcgatgc gcaatggtag tgatgtcgtg gatgagcacg ggtttggcgt
     2041 gaatgaggag gtcggccatc agcgaattgt cttctttgac ggggatcgtg agatgccggg
     2101 ccgcgtcagc gatctcgtcc gacacgcccg cgacttgcgc aacgtaggcc acgtcccgat
     2161 cctcgtgtcg tagcatcaac accatgcgcg agagtccaag attggtcatc agcaattgga
     2221 gcaccgtgtc catgagctca tggagattta aagtgctggt aatcgcggcg ctggtctgat
     2281 gcaccgtcgt caattggccg agctgccgct tgatcgtatc catgttggcg gtaatggcga
     2341 ggttgcgctc gttcaaggcc tgagtcatca cgttgaacat gtgagtgagc tcgccgactt
     2401 cgtcacgcgt ggaagaaagg agcggctcag gtaatttgcc ctctgcgacc tgatgagcga
     2461 ccgcggctaa tccgcgcaac ggggtggtga ttttggaggc caggatatgc gcaccgagaa
     2521 tgccggctgc aatgatgacc accgtgagca gaatgacatt ggtcaccatg ctgaggagtt
     2581 catgattcaa attggcgtcg ctcagcccga tttggacaag gccggatact tgcggaccat
     2641 ccggggaggg acgaggctgg ctgcgctcct ccagttggag cgagaacgga gcgatcgaac
     2701 ctccggaggg tgtcgtacga agcacgggca tcgcaaagtc atacacgcgt tcttcccagg
     2761 caaatcgagg gctcaccaca ttgacaatcg agaggggcgt catcttcggg gcggaatgcg
     2821 gtgtgctcag gagtgtgtgc gcaatgctct cgtccgggta caagggatac tccggggacc
     2881 gcaacaggct ggcgggggat cgacgtcccc ctttggtctc tttggtgatg accgcgccat
     2941 ccgggcccct cacgacgaca tacacgacat cctggacggc cataaggccg tcgacgaact
     3001 ccttgagcgt ggcgcgatct tgcgcgacga ggccggcata ccggaagtgt tcgttgccga
     3061 cgacgttcgt gaggaggatg gttcccaact gcctgaggtt ctcggccatg gccgcgcgcc
     3121 ggttctcgat caagtaccaa ctgagggtcg agcaggacac gatcagaatg agactaaaaa
     3181 agagcacgaa tttggtgcgc aggctgagga accgactctt gccggcattc atcggatcgt
     3241 cgagggcgtt ggaggatggt gaatgggcca tggctgaatc agtatgtttc gtcaataagg
     3301 tgattcactt ctttgggaaa ttcaataccc aggaaacggg ccgtcttgag attcacggta
     3361 atcgtcagcc gttcaatcgg caccggcttt gccggaagct tcttgtcccc gtccaggata
     3421 cgctttgcca acagccctgt ctcgcggccg acttccccgt acgagaccga caggctgagc
     3481 agggcgccca gtcgggtgaa ctcgggggag aatccgatca caggaatgcg gcgtgccaat
     3541 gaggattcga gaataaagcc gatcgactcg ttcgtgagga ccttcgaatc ggggatgagc
     3601 cagagtccct cgactgtcga cagcagcgaa cgcagttgtt gaggaacctc cttctcgctc
     3661 tcaacgggga aatcttggag tgtgaaccct tgggcagaag cttgcgcggt agcctctttg
     3721 aacttgcccg agctgttgcc ggggctggag agactgccga gccgtcgcag attgggcaga
     3781 aatgcgcgga ggattttgaa ctgacgctcc gccgggatct ctaggagcgt gcccgtcata
     3841 ttcgaagcgt tgaggtagtg tttcagaggg tccaggatca tcatatagac aaccggcgtg
     3901 tccacaatct cgagtttggc tgccagcgcg gctttaaggc cgacagccac gaccagggat
     3961 gtgtccgatg cgcgtatctt tctggcgagt ttcctgcctt gctccaggtc tccctgcaga
     4021 tcatattccg tgtagatgat tccggagggt gcagtcgctt tgaacccttc gatagcttgc
     4081 tcgtatgcgt tgatgccgga ggacttgaga atcgcaatgt ccatggccat ggattcgcca
     4141 tcgcacaggg cgaacaggct gaggaggacc gcgaacccca gcacaagcct gggagcgcgg
     4201 caggatcctg ttcggaggcg gttggctctg cgagtcatga agctcataga caatagacgg
     4261 ggaatcttca ctcctcctgt ctcacttctc tctcgtcttc ctggggcgag ccgacaggct
     4321 gcggaccatg cccaacatat gcacgacttt cccctcacgg tttcgaagaa cttgcccgct
     4381 ccaaacgagc caacggacgg atccatccgt cgtcaccgtc cgatgctcaa acagcacgtc
     4441 ttggccgccc tgctccaacg cctcatccaa ggcctgcgcc atggcttgcc ggtcgtccgg
     4501 atggacgaga gcaagccagt cctcccggga gcgcgcccgc gaaccatccg acaggccgag
     4561 catggtgtcc acttgccggg accagcagaa ttggccggtc tgacggtccc aatcccagcc
     4621 gccgacttct gcggatgtga gcgcgtgacg aagttgagcc tcatgggtcc ggagcgcatc
     4681 ttcggcgagc tttcgctccg tgatatcgga cgataccccg cagaccgcat acggggaccc
     4741 gtcctgaccg cgcaacggga acttgatcga gaggtaagta tgcagcccgt cctgatgcgg
     4801 ggcctgctct tcgtattcca ccgtcgcatt cttctcgaac acctcaatgt cgttcgcgcg
     4861 aaaggcatcg gcgatcttgc gggggaaaat ctcgtggttc gtgtgcccga taatctgatc
     4921 ggctgtcagg ttgaagagtt cctcgaaacg gcgattcacg aagaggaagc gtccatccgg
     4981 gtccttcgcg tagatgacgg cggtggtcgt gtcggcgact tcttgaagca attgctggct
     5041 tcggcgaatc gcctcttccg tccgcttgcg atccatgatc gattgcagtg agccggccat
     5101 gcggatcatg cggccgacgg catcccagat cgcctgcccg cgtgcgcgaa cccagtggta
     5161 gtccccctgt ttcgtcagca gccggtactc ttcatcgtag gggacacgtc gttcgatatg
     5221 cgcggtcagc gcggcaaaga cgcgctcgcg atcctcggga tgcagccgtg aagcccaact
     5281 cttcaggaca tcgggaaatt cctcctcggt atagccgagc atttcgcgca cgcgggaaga
     5341 ccaccagatc ggcgtgtcgg gagaattcca aggctttcct ggcagaactt gaccgtccca
     5401 aaacccgtcg ttcgagcccc gaatcaccag ctccagacgt tcttcgcttg cccggagcgc
     5461 cgactcggcc tttttgcggt cagtggtgtc gcggaacacc atgacgacgc cgaccaccct
     5521 gtcgtcttcc cgaatgggcg tgctgatgta ttcgaccggg aaactcgtgc cgtctttgcg
     5581 ccaaaacacc tcgtcattga ctcgatagac gactccgtcg cgaagtgagg cgtggacagg
     5641 acaatccttc
//
LOCUS       DATIFR010000264         6451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U16400 k141_1722306, whole
            genome shotgun sequence.
ACCESSION   DATIFR010000264 DATIFR010000000
VERSION     DATIFR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935894
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 05:48:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 3,013
            Genes (coding)                    :: 3,012
            CDSs (with protein)               :: 3,012
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722306"
                     /isolate="SMAG_U16400"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..516
                     /locus_tag="VLD60_15225"
     CDS             328..516
                     /locus_tag="VLD60_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46362.1"
                     /translation="MTELKPLTYDEHKAAEAAFRGSPPDPDWTDAAQSLYVKLSKAVA
                     DRAARLSVDHPPAAAALR"
     gene            complement(760..1563)
                     /gene="dapB"
                     /locus_tag="VLD60_15230"
     CDS             complement(760..1563)
                     /gene="dapB"
                     /locus_tag="VLD60_15230"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSB46363.1"
                     /translation="MIKVIVAGAAGRMGSRLVALIKDSTALTLAGAIEGKGHHALGED
                     AGEAAGCGKAGVQITEDLSPLLERGEVVIDFSSPEATLNHLRVVAQHRRAMVIGTTGF
                     AASELEELKSLARQVPCVFSPNMSVGVNLIYKVISEMARTLGDEYDIEVIEAHHRLKK
                     DAPSGTALKIAEVLARAVNRDLNQVGVYARKGLIGERKKQEIGIQTIRAGDIVGDHTI
                     LFGGMGERIEVTHRASSRDTFGRGALRAARWVVRQPPGLYDMMDVLGLK"
     gene            complement(1588..2460)
                     /gene="dapA"
                     /locus_tag="VLD60_15235"
     CDS             complement(1588..2460)
                     /gene="dapA"
                     /locus_tag="VLD60_15235"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSB46364.1"
                     /translation="MFTGSLVAIVTPFRKGQLDERAFGDLIEWQIANGTNGIVPCGTT
                     GESATLTHEEHHRMVKLAVEVARRRVPVIAGTGSNSTDEAISLTRHAKEAGADGALLI
                     TPYYNKPTQEGLYRHYKAVAESVEIPQILYNIPGRTGVNMLPTTVARLCGMKNIVGIK
                     EGSGLVQQASEIAQTCGERMTVLAGDDALTLPMMAVGAKGVITVAANLVPKEMAQLVS
                     TFLTGRVEESRKLHFMLSPLFAALFYETNPIPVKEALGMMGKIDPELRLPLCAMGADT
                     KAQLTRVLKDMKLI"
     gene            complement(2478..3740)
                     /gene="lysA"
                     /locus_tag="VLD60_15240"
     CDS             complement(2478..3740)
                     /gene="lysA"
                     /locus_tag="VLD60_15240"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSB46365.1"
                     /translation="MHNFEYRQGELYCEQVPVSRIAKEVGTPCYIYSHATLTRHFRAY
                     DSAFKNIPHIIAFAMKANSNLAILRLMAKEGSGVDIVSGGELFRALKAGVPSSKIVFA
                     GVGKNAEEIRDALKADILMFNVESSAEIHAINDVAASVGKKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRALEEFKLASSLGHIEVVGVHAHIGSQLTEVTPFVESLKKVI
                     TLIAALKEQGINIQYLNIGGGLGITYSDEKPPLPQDLSDAISPLVRDLKLTLVMEPGR
                     VIVGNAGILVTKALYEKVGEAKRFIIVDAAMNDLIRPSLYSAYHEIRPVFESLLQRPK
                     HSVDVVGPVCESGDFLAKDRTLPEVRPGDLLAVMSAGAYGFVMASNYNSRPRVPEVLI
                     KDGEIHIIKARETYDDLVRGETVPAFLE"
     gene            complement(3733..5643)
                     /locus_tag="VLD60_15245"
     CDS             complement(3733..5643)
                     /locus_tag="VLD60_15245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB46366.1"
                     /translation="MQLIQPMRQGHLPRQTAGKRLLLVDPYPRNSQYHLTASERRAVW
                     FPKLSLPTIAAYTPEHWTVDLVDEAVQDIDFDYPCDMVGLSIMTCYAPRAYEIAKEFR
                     KRGKTIVMGGVHPTYCPDEALQYADAIVCGEAEDLWPQLVADYEAGQMQRMYKMTSFP
                     ALENYKSPRVELLSPDAYMTRQCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVEEVVTE
                     LQNVQRWIRSELVERIRDEPLWKAFILSLRIRVGIEDGSIVAFVDDLHNSNRAYCREL
                     WTALKPLKLKWGCQSTLFLADDEEMVKLAAESGCVSVFVGMESLDEDCLDETNKPFNR
                     VKKFAQEIKMFHDYGIMVNPGIVFGFDNDDEAVFERAVDFLTKNQVELAYFNILTPLP
                     GTALFDRYNNAGRIFDKDWSKYDGKHVVFQPSRMTPEQLQEGFHWANHQFYSLPNIWY
                     RLSGTKQRLVARWEMNREFRKLVKRSCPKGTLSPLATVLKNLQAKLPVLDKDQLIPSA
                     LHALKQRLDPKTLSTENLTLNIKAKRHDKFAALFVDLEGALDQLNVQELIKRIKEAAE
                     NAKLDVIVNFEHLKLAAPDALKALIDSEAIKAAVPHVKVRYRKFKDAFETSLQGFSLA
                     GLEMLSEDFQDA"
     gene            complement(5703..5930)
                     /locus_tag="VLD60_15250"
     CDS             complement(5703..5930)
                     /locus_tag="VLD60_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB46367.1"
                     /translation="MRSLAVVLVMVLLAACGVVGPPIAPEDVGVSRTVEFQKKRDALE
                     AQQREAAESQAASEPQGQDVDLPPVRPFGTR"
     gene            complement(5927..>6451)
                     /locus_tag="VLD60_15255"
     CDS             complement(5927..>6451)
                     /locus_tag="VLD60_15255"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSB46368.1"
                     /translation="KNPDVPELVRGKTGRVYGHLINLLTMLKALPLSYNRDLQEDKPA
                     LFDAIDTVAASLQVLTELTRRLKVNKKALKQALQGGGMLATELADYLVARGVPFREAH
                     AITGRVVRAALDRGQELTSFSLEELRRFSHRIEKGVFARLTVQAAIDRKAQIGGTARV
                     RVEQRIKELERMLS"
BASE COUNT         1311 a         2007 c         1719 g         1414 t
ORIGIN      
        1 gaccacaatc ctatgggacg gggtagtccc tgtcaaggat cccgatccca ggcccaagcc
       61 tcacagtagc cgttgcctgc ttctaataac cggccaaccc cgcaaagatc ccctttcacc
      121 agtacttctg atggatatgc acacatttct gagagtgtta gagtacttgt atgaagacat
      181 gcgataaatg ccgcggaaca atgttaccgg aacgggcagt ggatttggat gccgggctcg
      241 cgatcatggt gtttgcctgt ttgaactgtg gtcggcgcaa agcagcggat caggaacctc
      301 gtccccttac ggccagacat tgacgcgatg acggaactca aaccgctcac gtacgatgag
      361 cataaggctg ccgaggcggc ctttcgtggc tctccgcctg atcccgattg gaccgacgcc
      421 gcgcagtcgc tctatgtgaa gctatcgaag gcggtcgctg atcgtgcagc tcgcttatct
      481 gtagatcatc caccagcggc agccgccctc cgttaatcgc attatgcggc ctcccgtcca
      541 taaactctcg cgggttccgg aaacggccct tctcaactgc ccctcctgct accgcatgat
      601 cagctacagt cctcagcaag ccacgatcgc ttgcacatgg tgcggcatcg ccatgaaaca
      661 gtccgcccct aagacgcggt aacttcatca cgttcagggc agcaacattt tacggtggtt
      721 ggaagaagtt gccgagtgga gcggcgaggg gtaccgccat tacttaaggc ctagcacatc
      781 catcatgtcg tacagcccgg gtggctgcct gacgacccat cgtgccgccc gcaacgcacc
      841 acgcccgaag gtgtcgcgac tgctggcccg atgggtcact tcgatccgtt ctcccatccc
      901 gccgaacaga atggtgtggt cgccgacaat atcgccggca cgaatcgttt gaatcccgat
      961 ctcctgcttc tttcgttctc caatcagacc ttttcgcgcg tagaccccga cttgattgag
     1021 atcccgattc acagccctgg ccaatacctc cgcgatcttg agcgccgtcc cgctcggcgc
     1081 atcttttttg agccggtgat gcgcctcaat gacctcgatg tcgtattcgt cccccaatgt
     1141 cctggccatc tcgctaatga ccttgtaaat caagtttacg cccacactca tgttggggga
     1201 aaagacgcag ggcacctggc gggccagcga tttcagctct tcgagttccg aggcggcgaa
     1261 acctgtcgtt ccgatcacca tggcacgccg gtgctgcgcc accacccgta gatgattcag
     1321 ggtcgcttcc ggagaggaaa aatcaatgac gacttctccc cgctcgagga gcggcgaaag
     1381 atcctccgta atctggacgc cggccttgcc acagcccgcc gcctcgccgg catcctcgcc
     1441 caacgcatgg tggcccttgc cctcgatcgc gcccgccaat gtgagcgcgg tggaatcctt
     1501 gatcaaggcg accaaccggc tgcccattcg acccgcggca ccagcgacaa tgactttgat
     1561 catgcagaac cgctcgcttc cgatcgacta gatcagcttc atgtccttca acacgcgcgt
     1621 taattgggct ttcgtgtcgg cccccatcgc acagagtggc agccgcaact ccggatcgat
     1681 tttccccatc atgcccaagg cttctttcac cgggatcggg ttcgtttcgt agaacagcgc
     1741 cgcgaaaagg ggcgacaaca tgaagtgcag cttgcgcgat tcctcgaccc ggcctgtcaa
     1801 aaacgtactg accagctgcg ccatctcctt cggaaccaga ttcgccgcca ctgtgatgac
     1861 cccttttgcc cccaccgcca tcatggggag ggtcaacgcg tcgtcaccgg ccaggaccgt
     1921 catccgctca ccgcaggtct gcgcaatttc agacgcttgc tggaccaagc cgcttccttc
     1981 cttaatgccc acgatgttct tcataccgca caatcgcgcg acggtcgtgg gcaacatatt
     2041 tacgccggtc cggccaggaa tattatagag gatctgcgga atctcgacgg actccgcgac
     2101 cgccttgtaa tgtcgataca gcccttcttg ggtcggcttg ttgtaatagg gcgtgatcag
     2161 cagcgccccg tccgcaccgg cctctttggc atgcctggtc aatgagatgg cttcgtcggt
     2221 gctgttggag ccggtccctg cgatgaccgg gacacgtcgc ctggcgactt cgacggccag
     2281 cttgaccatg cgatgatgtt cctcgtgcgt cagggttgcc gattcaccag tggtgccgca
     2341 aggaacaatc ccgttcgttc cattggcaat ctgccactcg atcaaatcgc cgaaagcccg
     2401 ctcatcaagc tggcccttcc tgaacggcgt gacgatggca accaacgacc cggtaaacat
     2461 gggaactcct ttaccgacta ctcgagaaat gcgggaaccg tttcacccct aaccagatca
     2521 tcgtaagttt ctcgtgcttt gatgatatgg atctcgccgt ccttgatcag cacctcaggc
     2581 acccgcggcc gcgagttgta gttggacgcc atgacaaacc cgtaggctcc cgcgctcatg
     2641 acggcaagca gatcgccagg cctgacctcc gggagcgtgc gatctttcgc caggaagtcg
     2701 ccggactcac agaccggccc gactacatcg accgagtgtt tggggcgctg caacagggat
     2761 tcgaagacgg gacggatctc gtgataggcg ctgtagaggc tcggcctgat caagtcgttc
     2821 atcgcggcat cgacgataat gaaccgcttc gcttcgccga ccttttcgta gagcgctttg
     2881 gtcacgagga tgcctgcatt ccccacgatc acgcgtcccg gctccatcac cagcgtgagt
     2941 ttcaagtccc gcacgagcgg tgaaatggcg tcggacagat cttgcggtaa tggcggcttc
     3001 tcgtcgctgt aggtgatccc gagcccaccc ccgatgttca ggtattgaat gttgatgccc
     3061 tgctccttca gtgctgcgat cagcgtgatc accttcttca aggattcgac gaagggcgtg
     3121 acctctgtca gttgcgatcc gatatgcgcg tgcaccccga cgacctcgat atggccgagc
     3181 gaggacgcca gcttgaattc ctccaaggca cgatcggcgg cgatcccgaa cttgctcttc
     3241 ttcaagccgg tcgagatgta gggatgcgtc ttgggatcga tgtcgggatt gatccgcaat
     3301 gctacgcggg ctttctttcc gaccgaagca gccacgtcgt tgatcgcgtg aatctccgcc
     3361 gacgactcca cgttgaacat gaggatgtcc gccttcagcg catcacgaat ctcctccgca
     3421 ttcttaccga cgccggcgaa cacgattttg gaggagggca caccggcctt cagcgcacga
     3481 aacaattcgc ccccggacac gatatcgacg ccgcttcctt ccttcgccat aagacgaagg
     3541 atcgccagat tcgaattggc tttcatcgca aacgcgatga tgtgcgggat gttcttgaac
     3601 gcgctgtcgt acgcgcggaa atgcctggtc aacgtcgcgt gactatagat gtagcagggc
     3661 gtgccaactt ctttggcaat ccggctgacg ggcacctgtt cacaatagag ctcgccctgg
     3721 cgatattcga aattatgcat cctgaaaatc ctcgctcagc atttcgagac ccgcaaggga
     3781 gaagccttgc agcgaggtct cgaaggcatc cttgaacttg cgatagcgca ccttcacatg
     3841 cggcacagcg gccttgatgg cctccgaatc gatcaaggcc ttcagcgcat ctggcgctgc
     3901 cagtttcagg tgctcgaaat tgacgatgac gtccaatttc gcgttctcgg ccgcctcttt
     3961 gatccgcttg atgagttcct gaacattcaa ctggtccagc gcaccctcga gatcaacaaa
     4021 gagggcggcg aacttatcat gccgttttgc tttgatgttc agggtgagat tttctgtcga
     4081 cagcgtcttc gggtctaagc gttgcttcaa ggcatgtaac gcactgggaa tgagttgatc
     4141 cttgtccaac acagggagct tggcttgcag atttttcagc acagtcgcca ggggggaaag
     4201 ggttcccttg ggacagctcc gcttaaccaa ttttctgaat tcccggttca tttcccatcg
     4261 ggccaccaac cgctgcttgg tgccggaaag ccgataccag atgttgggca acgaatagaa
     4321 ttgatggttg gcccaatgga atccctcttg tagctgttcc ggcgtcatgc gactcggctg
     4381 aaacacgacg tgtttcccgt cgtacttcga ccaatccttg tcgaagatcc ggccggcgtt
     4441 gttataccgg tcgaagagcg cggttcccgg caacggcgtg agaatgttga agtaggccaa
     4501 ctccacttga tttttggtca aaaaatcaac ggcgcgttca aacaccgctt cgtcgtcgtt
     4561 gtcgaacccg aacacgatgc caggattcac catgatccca taatcgtgga acattttgat
     4621 ctcttgggca aactttttga cgcggttgaa cggcttattg gtttcatcga ggcagtcttc
     4681 atccaacgat tccatcccga cgaacacgga gacgcagccg ctctccgccg ctagcttgac
     4741 catttcctca tcgtccgcca gaaacagcgt ggactggcag ccccatttga gcttgagcgg
     4801 cttcaacgcc gtccagagct cgcggcaata ggcgcggttg ctgttgtgca ggtcgtcgac
     4861 aaacgccacg atgctgccgt cttcgatgcc gactcgaatg cggagactca ggatgaaggc
     4921 cttccagagc ggttcgtctc gaatacgctc aaccaattcg ctgcgaatcc aacgttgcac
     4981 gttctggagc tccgtgacga cttcttccac cggcctgcgc cgcgtggtct tgccgttgaa
     5041 gggcgacaca ctgcaaaact cacaatcgaa gtgacaaccc ctggtcgtaa aactgcattg
     5101 cctcgtcata taggcatcgg ggctcaacaa ttcgacgcgc gggctcttgt aattctccaa
     5161 cgccggaaac gacgtcattt tgtacatccg ctgcatttga cccgcctcgt agtctgcgac
     5221 tagttgcggc cacaaatctt ctgcctcgcc gcagacgatc gcgtccgcat actgtaaggc
     5281 ttcgtctggg cagtacgtgg ggtggacgcc gcccattacg atggttttcc cccgcttgcg
     5341 aaactctttg gcgatctcat aggcgcgcgg cgcataacag gtcatgatcg aaaggccgac
     5401 catatcgcag ggatagtcga aatcgatgtc ctgcaccgct tcgtcgacca aatcgacggt
     5461 ccaatgctcc ggcgtatagg cggcaatcgt cgggaggctc aacttcggga accagaccgc
     5521 ccgtcgctcc gacgccgtca ggtggtactg gctgttgcga ggatagggat ccaccagcag
     5581 caaccgtttg cccgcggtct gtcggggcaa atggccttgt cgcatgggtt gaatcagttg
     5641 catcagtccc tcctccagtt caacatcggt gcaaggtgaa tagtcataac attttcccgc
     5701 gttcagcgcg taccgaacgg tcgtaccggc ggcaaatcca catcttggcc ttgcggttca
     5761 cttgccgcct gcgattcggc cgcttcacgt tgttgcgctt cgagggcatc acgcttcttc
     5821 tgaaactcga ccgtccggga caccccgaca tcttcaggag caatcggcgg ccccacaact
     5881 ccgcaggctg ccagcagcac catgaccagc acgacggcca gacttctcac gacaacatcc
     5941 gttcgagttc cttgatccgc tgctccacgc gcacccgagc cgtccccccg atctgtgcct
     6001 tacgatcaat cgccgcctga actgtcaggc gcgcgaagac ccctttttcg atacgatggg
     6061 agaaccggcg caactcttcg agcgagaacg aggtcaattc ctgcccgcga tccaacgcgg
     6121 cccgcacgac cctccccgta atggcgtggg cctcgcggaa gggcacgccc ctcgccacca
     6181 gatagtcggc caactccgtc gcgagcattc cgccgccttg gagagcctgc ttgagcgcct
     6241 ttttgtttac tttgagccga cgcgtcagct cagtcaagac ctgcagggaa gccgccaccg
     6301 tatcgatcgc gtcgaacagg gccggcttat cctcttgtaa gtcccgatta taactgagcg
     6361 gcagggcctt cagcatggtc aacagattga tcaaatggcc gtagacccgg cctgtcttgc
     6421 cgcggaccaa ttcgggaaca tccggattct t
//
